LOCUS       DAZKFZ010000001       100461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000001 DAZKFZ010000000
VERSION     DAZKFZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(138..713)
                     /gene="gmhB"
                     /locus_tag="ACHOQW_000001"
     CDS             complement(138..713)
                     /gene="gmhB"
                     /locus_tag="ACHOQW_000001"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP9571156.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            901..1932
                     /gene="metN"
                     /locus_tag="ACHOQW_000002"
     CDS             901..1932
                     /gene="metN"
                     /locus_tag="ACHOQW_000002"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP9571157.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1925..2578
                     /gene="metI"
                     /locus_tag="ACHOQW_000003"
     CDS             1925..2578
                     /gene="metI"
                     /locus_tag="ACHOQW_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP9571158.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2618..3433
                     /gene="metQ"
                     /locus_tag="ACHOQW_000004"
     CDS             2618..3433
                     /gene="metQ"
                     /locus_tag="ACHOQW_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP9571159.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3551..3955
                     /gene="rcsF"
                     /locus_tag="ACHOQW_000005"
     CDS             3551..3955
                     /gene="rcsF"
                     /locus_tag="ACHOQW_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP9571160.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            3952..4659
                     /gene="tsaA"
                     /locus_tag="ACHOQW_000006"
     CDS             3952..4659
                     /gene="tsaA"
                     /locus_tag="ACHOQW_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP9571161.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            4770..6488
                     /gene="proS"
                     /locus_tag="ACHOQW_000007"
     CDS             4770..6488
                     /gene="proS"
                     /locus_tag="ACHOQW_000007"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9571162.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6541..7365
                     /gene="yaeF"
                     /locus_tag="ACHOQW_000008"
     CDS             6541..7365
                     /gene="yaeF"
                     /locus_tag="ACHOQW_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP9571163.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(7565..8275)
                     /gene="nlpE"
                     /locus_tag="ACHOQW_000009"
                     /gene_synonym="cutF"
     CDS             complement(7565..8275)
                     /gene="nlpE"
                     /locus_tag="ACHOQW_000009"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP9571164.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(8289..8699)
                     /gene="arfB"
                     /locus_tag="ACHOQW_000010"
     CDS             complement(8289..8699)
                     /gene="arfB"
                     /locus_tag="ACHOQW_000010"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP9571165.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            complement(8696..9241)
                     /gene="yaeQ"
                     /locus_tag="ACHOQW_000011"
     CDS             complement(8696..9241)
                     /gene="yaeQ"
                     /locus_tag="ACHOQW_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP9571166.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9407..9607
                     /gene="yaeP"
                     /locus_tag="ACHOQW_000012"
     CDS             9407..9607
                     /gene="yaeP"
                     /locus_tag="ACHOQW_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP9571167.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9594..9854
                     /gene="rof"
                     /locus_tag="ACHOQW_000013"
     CDS             9594..9854
                     /gene="rof"
                     /locus_tag="ACHOQW_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP9571168.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9907..11202)
                     /gene="tilS"
                     /locus_tag="ACHOQW_000014"
     CDS             complement(9907..11202)
                     /gene="tilS"
                     /locus_tag="ACHOQW_000014"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9571169.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(11267..11656)
                     /gene="yaeR"
                     /locus_tag="ACHOQW_000015"
     CDS             complement(11267..11656)
                     /gene="yaeR"
                     /locus_tag="ACHOQW_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9571170.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11713..13854)
                     /gene="ldcC"
                     /locus_tag="ACHOQW_000016"
     CDS             complement(11713..13854)
                     /gene="ldcC"
                     /locus_tag="ACHOQW_000016"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP9571171.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13953..14912)
                     /gene="accA"
                     /locus_tag="ACHOQW_000017"
     CDS             complement(13953..14912)
                     /gene="accA"
                     /locus_tag="ACHOQW_000017"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9571172.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14925..18407)
                     /gene="dnaE"
                     /locus_tag="ACHOQW_000018"
     CDS             complement(14925..18407)
                     /gene="dnaE"
                     /locus_tag="ACHOQW_000018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9571173.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18444..19040)
                     /gene="rnhB"
                     /locus_tag="ACHOQW_000019"
     CDS             complement(18444..19040)
                     /gene="rnhB"
                     /locus_tag="ACHOQW_000019"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9571174.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(19037..20185)
                     /gene="lpxB"
                     /locus_tag="ACHOQW_000020"
     CDS             complement(19037..20185)
                     /gene="lpxB"
                     /locus_tag="ACHOQW_000020"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP9571175.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20185..20973)
                     /gene="lpxA"
                     /locus_tag="ACHOQW_000021"
     CDS             complement(20185..20973)
                     /gene="lpxA"
                     /locus_tag="ACHOQW_000021"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP9571176.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20977..21432)
                     /gene="fabZ"
                     /locus_tag="ACHOQW_000022"
     CDS             complement(20977..21432)
                     /gene="fabZ"
                     /locus_tag="ACHOQW_000022"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9571177.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21537..22562)
                     /gene="lpxD"
                     /locus_tag="ACHOQW_000023"
     CDS             complement(21537..22562)
                     /gene="lpxD"
                     /locus_tag="ACHOQW_000023"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9571178.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22566..23051)
                     /gene="skp"
                     /locus_tag="ACHOQW_000024"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22566..23051)
                     /gene="skp"
                     /locus_tag="ACHOQW_000024"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP9571179.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23172..25604)
                     /gene="bamA"
                     /locus_tag="ACHOQW_000025"
     CDS             complement(23172..25604)
                     /gene="bamA"
                     /locus_tag="ACHOQW_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP9571180.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25634..26986)
                     /gene="rseP"
                     /locus_tag="ACHOQW_000026"
     CDS             complement(25634..26986)
                     /gene="rseP"
                     /locus_tag="ACHOQW_000026"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP9571181.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDSIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(26998..27855)
                     /gene="cdsA"
                     /locus_tag="ACHOQW_000027"
     CDS             complement(26998..27855)
                     /gene="cdsA"
                     /locus_tag="ACHOQW_000027"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9571182.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27868..28626)
                     /gene="ispU"
                     /locus_tag="ACHOQW_000028"
                     /gene_synonym="uppS"
     CDS             complement(27868..28626)
                     /gene="ispU"
                     /locus_tag="ACHOQW_000028"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP9571183.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28815..30011)
                     /gene="ispC"
                     /locus_tag="ACHOQW_000029"
     CDS             complement(28815..30011)
                     /gene="ispC"
                     /locus_tag="ACHOQW_000029"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP9571184.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            complement(30103..30660)
                     /gene="frr"
                     /locus_tag="ACHOQW_000030"
     CDS             complement(30103..30660)
                     /gene="frr"
                     /locus_tag="ACHOQW_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9571185.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30810..31535)
                     /gene="pyrH"
                     /locus_tag="ACHOQW_000031"
     CDS             complement(30810..31535)
                     /gene="pyrH"
                     /locus_tag="ACHOQW_000031"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9571186.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31682..32533)
                     /gene="tsf"
                     /locus_tag="ACHOQW_000032"
     CDS             complement(31682..32533)
                     /gene="tsf"
                     /locus_tag="ACHOQW_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9571187.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32791..33516)
                     /gene="rpsB"
                     /locus_tag="ACHOQW_000033"
     CDS             complement(32791..33516)
                     /gene="rpsB"
                     /locus_tag="ACHOQW_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9571188.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33884..34678
                     /gene="map"
                     /locus_tag="ACHOQW_000034"
     CDS             33884..34678
                     /gene="map"
                     /locus_tag="ACHOQW_000034"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9571189.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34740..37412
                     /gene="glnD"
                     /locus_tag="ACHOQW_000035"
     CDS             34740..37412
                     /gene="glnD"
                     /locus_tag="ACHOQW_000035"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP9571190.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEDTRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37443..38267
                     /gene="dapD"
                     /locus_tag="ACHOQW_000036"
     CDS             37443..38267
                     /gene="dapD"
                     /locus_tag="ACHOQW_000036"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP9571191.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38582..38968
                     /gene="yaeH"
                     /locus_tag="ACHOQW_000037"
     CDS             38582..38968
                     /gene="yaeH"
                     /locus_tag="ACHOQW_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP9571192.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39057..40214)
                     /gene="cdaR"
                     /locus_tag="ACHOQW_000038"
     CDS             complement(39057..40214)
                     /gene="cdaR"
                     /locus_tag="ACHOQW_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP9571193.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40369..41793)
                     /gene="degP"
                     /locus_tag="ACHOQW_000039"
     CDS             complement(40369..41793)
                     /gene="degP"
                     /locus_tag="ACHOQW_000039"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP9571194.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(41923..43440)
                     /gene="dgt"
                     /locus_tag="ACHOQW_000040"
     CDS             complement(41923..43440)
                     /gene="dgt"
                     /locus_tag="ACHOQW_000040"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP9571195.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFSVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43524..44222
                     /gene="mtnN"
                     /locus_tag="ACHOQW_000041"
     CDS             43524..44222
                     /gene="mtnN"
                     /locus_tag="ACHOQW_000041"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9571196.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44215..45015
                     /gene="btuF"
                     /locus_tag="ACHOQW_000042"
     CDS             44215..45015
                     /gene="btuF"
                     /locus_tag="ACHOQW_000042"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP9571197.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45053..45676
                     /gene="yadS"
                     /locus_tag="ACHOQW_000043"
     CDS             45053..45676
                     /gene="yadS"
                     /locus_tag="ACHOQW_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP9571198.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45723..46067)
                     /gene="erpA"
                     /locus_tag="ACHOQW_000044"
     CDS             complement(45723..46067)
                     /gene="erpA"
                     /locus_tag="ACHOQW_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP9571199.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46060..46125)
                     /gene="yadW"
                     /locus_tag="ACHOQW_000045"
     CDS             complement(46060..46125)
                     /gene="yadW"
                     /locus_tag="ACHOQW_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP9571200.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46149..47570)
                     /gene="clcA"
                     /locus_tag="ACHOQW_000046"
     CDS             complement(46149..47570)
                     /gene="clcA"
                     /locus_tag="ACHOQW_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP9571201.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(47582..47629)
                     /gene="yadX"
                     /locus_tag="ACHOQW_000047"
     CDS             complement(47582..47629)
                     /gene="yadX"
                     /locus_tag="ACHOQW_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP9571202.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            47795..49075
                     /gene="hemL"
                     /locus_tag="ACHOQW_000048"
     CDS             47795..49075
                     /gene="hemL"
                     /locus_tag="ACHOQW_000048"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP9571203.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49110..51092)
                     /gene="fhuB"
                     /locus_tag="ACHOQW_000049"
     CDS             complement(49110..51092)
                     /gene="fhuB"
                     /locus_tag="ACHOQW_000049"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP9571204.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGGALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51089..51979)
                     /gene="fhuD"
                     /locus_tag="ACHOQW_000050"
     CDS             complement(51089..51979)
                     /gene="fhuD"
                     /locus_tag="ACHOQW_000050"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP9571205.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(51979..52776)
                     /gene="fhuC"
                     /locus_tag="ACHOQW_000051"
     CDS             complement(51979..52776)
                     /gene="fhuC"
                     /locus_tag="ACHOQW_000051"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP9571206.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52827..55070)
                     /gene="fhuA"
                     /locus_tag="ACHOQW_000052"
     CDS             complement(52827..55070)
                     /gene="fhuA"
                     /locus_tag="ACHOQW_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP9571207.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(55290..57824)
                     /gene="mrcB"
                     /locus_tag="ACHOQW_000053"
     CDS             complement(55290..57824)
                     /gene="mrcB"
                     /locus_tag="ACHOQW_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP9571208.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(58020..60449)
                     /gene="hrpB"
                     /locus_tag="ACHOQW_000054"
     CDS             complement(58020..60449)
                     /gene="hrpB"
                     /locus_tag="ACHOQW_000054"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP9571209.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60523..61053
                     /gene="thpR"
                     /locus_tag="ACHOQW_000055"
     CDS             60523..61053
                     /gene="thpR"
                     /locus_tag="ACHOQW_000055"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP9571210.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            61068..61772
                     /gene="sfsA"
                     /locus_tag="ACHOQW_000056"
     CDS             61068..61772
                     /gene="sfsA"
                     /locus_tag="ACHOQW_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9571211.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            61950..62405
                     /gene="dksA"
                     /locus_tag="ACHOQW_000057"
     CDS             61950..62405
                     /gene="dksA"
                     /locus_tag="ACHOQW_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP9571212.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62472..63368
                     /gene="gluQRS"
                     /locus_tag="ACHOQW_000058"
     CDS             62472..63368
                     /gene="gluQRS"
                     /locus_tag="ACHOQW_000058"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP9571213.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            63428..64825
                     /gene="pcnB"
                     /locus_tag="ACHOQW_000059"
     CDS             63428..64825
                     /gene="pcnB"
                     /locus_tag="ACHOQW_000059"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP9571214.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            64822..65304
                     /gene="folK"
                     /locus_tag="ACHOQW_000060"
     CDS             64822..65304
                     /gene="folK"
                     /locus_tag="ACHOQW_000060"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP9571215.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            65585..66181
                     /locus_tag="ACHOQW_000061"
     CDS             65585..66181
                     /locus_tag="ACHOQW_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9571216.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            66268..66993
                     /locus_tag="ACHOQW_000062"
     CDS             66268..66993
                     /locus_tag="ACHOQW_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9571217.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            67035..69635
                     /locus_tag="ACHOQW_000063"
     CDS             67035..69635
                     /locus_tag="ACHOQW_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFP9571218.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            69649..70197
                     /locus_tag="ACHOQW_000064"
     CDS             69649..70197
                     /locus_tag="ACHOQW_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9571219.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            70212..70817
                     /locus_tag="ACHOQW_000065"
     CDS             70212..70817
                     /locus_tag="ACHOQW_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9571220.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            70838..71428
                     /locus_tag="ACHOQW_000066"
     CDS             70838..71428
                     /locus_tag="ACHOQW_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9571221.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            71440..72549
                     /locus_tag="ACHOQW_000067"
     CDS             71440..72549
                     /locus_tag="ACHOQW_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9571222.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            72684..73478
                     /gene="panB"
                     /locus_tag="ACHOQW_000068"
     CDS             72684..73478
                     /gene="panB"
                     /locus_tag="ACHOQW_000068"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP9571223.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            73490..74341
                     /gene="panC"
                     /locus_tag="ACHOQW_000069"
     CDS             73490..74341
                     /gene="panC"
                     /locus_tag="ACHOQW_000069"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP9571224.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(74640..75536)
                     /gene="rpnC"
                     /locus_tag="ACHOQW_000070"
     CDS             complement(74640..75536)
                     /gene="rpnC"
                     /locus_tag="ACHOQW_000070"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP9571225.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            75810..76190
                     /gene="panD"
                     /locus_tag="ACHOQW_000071"
     CDS             75810..76190
                     /gene="panD"
                     /locus_tag="ACHOQW_000071"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP9571226.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(76194..77423)
                     /gene="yadE"
                     /locus_tag="ACHOQW_000072"
     CDS             complement(76194..77423)
                     /gene="yadE"
                     /locus_tag="ACHOQW_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9571227.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(77487..77927)
                     /gene="yadI"
                     /locus_tag="ACHOQW_000073"
     CDS             complement(77487..77927)
                     /gene="yadI"
                     /locus_tag="ACHOQW_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9571228.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(78032..78802)
                     /gene="yadH"
                     /locus_tag="ACHOQW_000074"
     CDS             complement(78032..78802)
                     /gene="yadH"
                     /locus_tag="ACHOQW_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9571229.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(78799..79725)
                     /gene="yadG"
                     /locus_tag="ACHOQW_000075"
     CDS             complement(78799..79725)
                     /gene="yadG"
                     /locus_tag="ACHOQW_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9571230.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            79834..80496
                     /gene="can"
                     /locus_tag="ACHOQW_000076"
     CDS             79834..80496
                     /gene="can"
                     /locus_tag="ACHOQW_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP9571231.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(80537..81073)
                     /gene="hpt"
                     /locus_tag="ACHOQW_000077"
     CDS             complement(80537..81073)
                     /gene="hpt"
                     /locus_tag="ACHOQW_000077"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9571232.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            81279..83669
                     /gene="gcd"
                     /locus_tag="ACHOQW_000078"
     CDS             81279..83669
                     /gene="gcd"
                     /locus_tag="ACHOQW_000078"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP9571233.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(83871..85421)
                     /gene="cueO"
                     /locus_tag="ACHOQW_000079"
     CDS             complement(83871..85421)
                     /gene="cueO"
                     /locus_tag="ACHOQW_000079"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP9571234.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            85587..85934
                     /gene="yacC"
                     /locus_tag="ACHOQW_000080"
     CDS             85587..85934
                     /gene="yacC"
                     /locus_tag="ACHOQW_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP9571235.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            86040..86906
                     /gene="speE"
                     /locus_tag="ACHOQW_000081"
     CDS             86040..86906
                     /gene="speE"
                     /locus_tag="ACHOQW_000081"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP9571236.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            86922..87716
                     /gene="speD"
                     /locus_tag="ACHOQW_000082"
     CDS             86922..87716
                     /gene="speD"
                     /locus_tag="ACHOQW_000082"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP9571237.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(87754..88116)
                     /gene="yacL"
                     /locus_tag="ACHOQW_000083"
     CDS             complement(87754..88116)
                     /gene="yacL"
                     /locus_tag="ACHOQW_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP9571238.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(88292..90889)
                     /gene="acnB"
                     /locus_tag="ACHOQW_000084"
     CDS             complement(88292..90889)
                     /gene="acnB"
                     /locus_tag="ACHOQW_000084"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP9571239.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            91244..93097
                     /gene="yacH"
                     /locus_tag="ACHOQW_000085"
     CDS             91244..93097
                     /gene="yacH"
                     /locus_tag="ACHOQW_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP9571240.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(93168..94592)
                     /gene="lpdA"
                     /locus_tag="ACHOQW_000086"
     CDS             complement(93168..94592)
                     /gene="lpdA"
                     /locus_tag="ACHOQW_000086"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9571241.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(94800..96692)
                     /gene="aceF"
                     /locus_tag="ACHOQW_000087"
     CDS             complement(94800..96692)
                     /gene="aceF"
                     /locus_tag="ACHOQW_000087"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9571242.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(96707..99370)
                     /gene="aceE"
                     /locus_tag="ACHOQW_000088"
     CDS             complement(96707..99370)
                     /gene="aceE"
                     /locus_tag="ACHOQW_000088"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP9571243.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(99531..100295)
                     /gene="pdhR"
                     /locus_tag="ACHOQW_000089"
     CDS             complement(99531..100295)
                     /gene="pdhR"
                     /locus_tag="ACHOQW_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP9571244.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT        24337 a        27309 c        24836 g        23979 t
ORIGIN      
        1 attttttctc ttcaaccgaa ccggctgttt gtgtgaagtg attcacattc gccgtgtcga
       61 tggaggcgca ttatagggag tcggctcagg aagacaagcg gaaaaatgca tttttatttc
      121 aaccgctcat cttttaatca ttgcgccggt ttttgctgct tttttatcgc ttgcggcagg
      181 tctgccaggc tatttaacac ccaatctgcc gcgttttctg cttcaggtgt aataggttta
      241 cccgtacgca ccagcacttt tgttcccacg ttcgccgcaa ccgctgcctg catatcttct
      301 aatttatcgc ccaccatata agaagcggcc atatcaatat gcaaataatc gcgtgctgac
      361 aaaagcatcc ccggatgtgg tttgcggcaa tcgcagacct ggcgaaactc ttcaacacta
      421 ccctgcggat gatgcgggca ataatagata ccatccagat cgacatctcg gtccgccagc
      481 gaccagtcca tccactcggt cagcgtttca aactgtgctt cggtaaattt accgcgagca
      541 atgccagact ggttggtgac taccaccagc gcaaagccca ttttttttag ctcgcgcatg
      601 gcgtcaataa caccgtcgat aaattcaaag ttgtcgatct catggacata gccgtgatcg
      661 acattaatgg tgccatcacg gtcaagaaaa attgcgggta cgctcttcgc caccttttat
      721 agctccttaa taaggcatgt gacgctagta tcgcatgttt cgacctgcaa gaaagtgctc
      781 ttcgcataaa cctgattgat ttagacgtct ggatgcctta acatccattt cattgacggc
      841 gttgcccgtt tcaggcattc gagatgccac gactaactta atgacgataa taaataatca
      901 atgataaaac tttcgaatat caccaaagtg ttccaccagg gcacccgcac catccaggcg
      961 ttgaacaacg tcagcctgca tgtgccagct ggacaaattt atggcgttat cggtgcctca
     1021 ggcgcgggta agagtacgct tatacgttgt gtaaacctgc tggagcgccc aaccgagggt
     1081 agcgtgctgg tcgatggaca ggaactgacc acgctgtcag aatccgagtt gaccaaagct
     1141 cgccgccaga ttggtatgat tttccagcat tttaacctgc tctcttcgcg tactgttttt
     1201 ggcaacgtgg ctctgccgct ggagctggac aatacaccga aagacgagat caaacgtcgc
     1261 gtgacggaat tgctgtcatt ggttggtctt ggagataagc atgatagcta cccgtcgaat
     1321 ctttccggtg ggcagaaaca acgtgtggcg attgcccgtg cattagccag caatcccaaa
     1381 gtattgctgt gtgatgaagc caccagcgcg ctagacccgg caacgacacg ttctattctc
     1441 gaactgctga aagacattaa ccgccgtctg ggtttgacga ttctgttgat cacccacgaa
     1501 atggacgttg tgaagcgtat ttgtgattgc gttgcggtca tcagcaatgg cgaactgatc
     1561 gagcaggaca cggtaagtga agtgttctca catccgaaaa cgccgctggc gcagaagttt
     1621 attcagtcaa ccctgcatct ggatatcccg gaagattacc aggaacgtct gcaagcggag
     1681 ccatttactg actgcgtgcc gatgctgcgt ctggagttta ccggtcaatc ggtcgatgcc
     1741 ccactgcttt ctgaaaccgc gcgtcgtttc aacgtcaaca acaacattat tagcgcgcag
     1801 atggattacg ccggtggcgt taagttcggc atcatgctga ctgaaatgca cggcacacaa
     1861 caagatacgc aagccgccat tgcctggctg caggaacacc atgtaaaagt agaggtactg
     1921 ggttatgtct gagccgatga tgtggctgct ggttcgtggc gtatgggaaa cgctggcaat
     1981 gaccttcgta tccggatttt ttgggtttgt ggttggtctg ccggttggcg tcctgcttta
     2041 tgtcacgcgt ccggggcaaa ttattgctaa cgcgaagctg tatcgtaccg tttctgcgat
     2101 tgtgaacatt ttccgttcca tcccgttcat tatcttgctg gtatggatga ttccgtttac
     2161 ccgcgttatt gtcggtacat cgattggttt gcaggcagca attgttccgt taaccgttgg
     2221 tgcagcaccg tttattgccc gtatggtcga gaacgctctg ctggagatcc caaccgggtt
     2281 aattgaagct tcccgcgcaa tgggtgccac gccaatgcag atcgtccgta aagtgctgtt
     2341 accggaagcg ttgccgggtc tggtgaatgc ggcaactatc accctgatta ccctggtcgg
     2401 ttattccgcg atgggtggtg cagtcggtgc cggtggttta ggtcagattg gctatcagta
     2461 tggctacatc ggctataacg cgacggtgat gaatacggta ctggtattgc tggtcattct
     2521 ggtttattta attcagttcg caggcgaccg catcgtccgg gctgtcactc gcaagtaacg
     2581 ttcaacacaa cataaataat tgaagaagga ataaggtatg gcgttcaaat tcaaaacctt
     2641 tgcggcagtg ggagccctga ttggatcact ggcactggta ggctgcggtc aggatgaaaa
     2701 agatccaaac cacattaaag tcggcgtgat tgttggtgcc gaacagcagg ttgcagaagt
     2761 cgcgcagaaa gttgcgaaag acaaatatgg cctggacgtt gagctggtaa ccttcaacga
     2821 ctatgttctg ccaaacgaag cattgagcaa aggcgatatc gacgccaacg ccttccagca
     2881 taaaccgtac cttgatcagc aactgaaaga tcgtggctac aaactggtcg cagtaggcaa
     2941 cacttttgtt tatccgattg ctggttactc caagaaaatc aaatcactgg atgaactgca
     3001 ggatggttcg caggttgccg tgccaaacga tccaactaac cttggtcgtt cactgctgct
     3061 gctgcaaaaa gtgggcttga tcaaactgaa agatggcgtt ggcctgctgc cgaccgttct
     3121 tgatgttgtt gagaacccaa aaaatctgaa aattgttgaa ctggaagcac cgcaactgcc
     3181 gcgttctctg gacgacgcgc aaatcgctct ggcagttatt aacaccacct atgccagcca
     3241 gattggcctg actccagcga aaaacggtat ctttgtcgaa gataaagact ccccgtacgt
     3301 aaacctgatc gtaacgcgtg aagataacaa agacgccgag aacgtgaaga aattcgtcca
     3361 ggcttatcag tctgacgaag tttacgaagc agcaaacaaa gtgtttaacg gcggcgctgt
     3421 taaaggctgg taatttttag gctgtttcca caatttgtaa tatcattcag gacgggcgct
     3481 tgcccgtctt gtcattttta caagctcctg attcaatatt gacgttttga tcatacattg
     3541 aggaaatact atgcgtgctt taccgatctg tttagtagca ctcatgctaa gcggctgttc
     3601 catgttaagc agatcccctg tcgaacccgt tcaaagcact gcaccccagc cgaaagcgga
     3661 gcctgcaaaa ccgaaagcgc cgcgcgccac gccggtccga atttatacca atgcagaaga
     3721 attagtcggc aaaccgttcc gcgatctcgg tgaagtcagt ggcgactctt gccaggcctc
     3781 taatcaggac tctccgccga gcattccaac cgcgcgtaag cggatgcaaa tcaacgcctc
     3841 gaaaatgaaa gccaatgcgg tattactgca tagctgcgaa gttaccagcg gtacgccagg
     3901 ctgctatcgt caggctttat gtatcggttc tgcgcttaac attacggcga aatgagcagt
     3961 ttccagttcg agcaaatagg tgttattcgc tcgccctata aagaaaaatt cgccgttccg
     4021 cgccagccag gtctggtaaa aagcgccaac ggcgaactac atctcattgc tccctacaac
     4081 caggctgacg ccgttcgcgg cctggaagca ttcagccatt tatggatcct tttcgtcttt
     4141 catcaaacga tggaaggcgg ctggcgtccg actgtgcgtc cgcctcgtct tggcggtaat
     4201 gccagaatgg gtgttttcgc tacgcgctcc actttccgcc ccaacccgat tggtatgtcg
     4261 ctggtagagc tgaaagaggt tgtttgccat aaagactgcg tgattctgaa gctcggcagc
     4321 ctggatctgg tcgatggtac gccggtagtg gatatcaaac cgtatctccc ctttgccgaa
     4381 tcgcttcccg atgccagtgc cagctatgcg caaagtgcgc cagctgcaga gatggcggtg
     4441 agttttaccg cagaggtcga aaagcagctt ttgacgctgg agaagcgtta tccgcagtta
     4501 acgctgttta tccgcgaagt actggcgcag gacccgcgcc cggcctatcg taaaggtgag
     4561 gaaacgggca aaacctatgc cgtctggcta catgatttta acgttcgctg gcgcgtcacc
     4621 gacgcaggtt ttgaagtctt tgcgctggaa ccgcgttaaa ttcacgccct tctcttttga
     4681 catttctttt gcactggtaa actaaatcac tttttttgtc ccaggctcgc cttgagcctg
     4741 ttcgaccttc caactggaac cgtaacaaca tgcgtactag ccaatacctg ctctccactc
     4801 tcaaggagac acctgccgac gccgaggtga tcagccatca gctgatgctg cgcgccggga
     4861 tgatccgcaa gctggcctcc gggttatata cctggctgcc gaccggcgtg cgcgttctga
     4921 aaaaagtcga aaacatcgtg cgtgaagaga tgaacaacgc cggtgcaatc gaggtgtcga
     4981 tgccggtggt tcagccagcc gatttgtggc aagagagtgg tcgttgggaa cagtacggtc
     5041 cggaactgct gcgttttgtt gaccgtggcg agcgtccgtt cgtactcggc ccaactcatg
     5101 aagaagttat cactgacctg attcgtaacg agcttagctc ttacaaacag ctgccgctga
     5161 acttctatca gatccagacc aagttccgcg acgaagtgcg tccgcgtttc ggcgtcatgc
     5221 gttcccgcga attcctgatg aaagatgctt actctttcca tacttctcag gaatccctgc
     5281 aggaaaccta cgatgcaatg tatgcggcct acagcaaaat cttcagccgc atggggctgg
     5341 atttccgcgc cgtacaggcc gacaccggtt ctatcggcgg cagcgcctct cacgaattcc
     5401 aggtgctggc gcagagcggt gaagacgatg tggtcttctc cgacacctct gactatgcag
     5461 cgaacattga gctggcagaa gctatcgcgc cgaaagaacc gcgcgctgct gctacccagg
     5521 aaatgacgct ggttgatacg ccgaacgcga aaaccatcgc ggaactggtt gaacagttca
     5581 atctgccgat taagaaaacg gttaagactc tgctggttaa agcggttgaa ggcagtagct
     5641 tcccgctggt tgcgctgctg gtgcgcggtg atcacgagct gaacgaagtt aaagcagaaa
     5701 aactgccgca ggttgccagc ccgctgactt tcgcgaccga agaagaaatt cgtgccgtgg
     5761 ttaaagccgg tccgggttca ctgggtccgg taaacatgcc gattccggtg gtgattgacc
     5821 gtaccgttgc ggcgatgagt gatttcactg ctggtgctaa catcgatggt aaacactact
     5881 tcggcatcaa ctgggatcgc gatgtcgcta ccccggaagt tgcggatatc cgtaacgtgg
     5941 tggctggcga tccaagcccg gatggccagg gtacgctgct gatcaaacgt ggtatcgaag
     6001 ttggtcacat cttccagctg ggtaccaagt actccgaagc actgaaagcc tccgtacagg
     6061 gtgaagatgg ccgtaaccaa atcctgacta tgggttgcta cggtatcggg gtaacgcgcg
     6121 tagtggctgc ggcgattgag cagaactacg acgagcgcgg catcgtatgg cctgacgcta
     6181 tcgcgccgtt ccaggtggca attctgccga tgaacatgca caaatccttc cgcgtacaag
     6241 agcttgctga gaaactgtac agcgaactgc gtgcacaagg tatcgaagtg ctgctggatg
     6301 accgaaaaga acgtccgggc gtgatgtttg ccgatatgga actgatcggt attccgcaca
     6361 ctattgtgct gggcgaccgt aacctcgaca acgacgatat cgaatataaa tatcgtcgca
     6421 acggcgagaa acagttaatt aagactggtg acatcgtcga atatctggtg aaacagatta
     6481 aaggctgatg ccagaaaggg tcctgaattt cagggccctt ttttacatgg attgtataaa
     6541 atggataaac caaaggcgta ctgccgcctg cttctcccct gctttctttt actttcggcc
     6601 tgtaccgttg atatcagcca gccagactca tcagccacag cagtagatgc cgaggcgaaa
     6661 acatgggctg tcaaattcca gcatcaaagc tctttcaccg aacaatcaat aaaagaaata
     6721 acggtacctg atctcaaacc cggcgatttg ctgttctcct caagcctgga ggtaacctca
     6781 tttgggatcc gcgttttcag cacttcctct gtgagtcacg ttgcaatcta tctgggtgaa
     6841 aataacgttg cagaagcgac aggcgctggc gtccagattg tttcccttaa aaaagcgatg
     6901 aagcatagtg ataaactttt cgtcttacga gtcccggatc ttaccccgca acaagccaca
     6961 gaaatcaccg cgttcgccaa taaaatcaaa gatagcggtt ataactatcg cggcattgtc
     7021 gaatttattc cctttatggt gactcgccag atgtgctcac tgaatccgtt ctccgaggat
     7081 tttcgccaac agtgcgtcag cggcctggcg aaagcgcagt taagcagtgt ggtcgaagga
     7141 gacaaaaagt cgtggttttg ttcggaattt gtcactgatg catttgccaa agccgggcat
     7201 ccgcttacgc tggcgcaatc gggctggatt agccctgccg atctgatgca tatgcgtatt
     7261 ggtgatgtct ctgcgtttaa gccagaaacg cagttacagt atgttgggca cctgaagcca
     7321 gggatttata tcaaggcggg tcgctttgtt ggtttgaccc ggtgataaag taaatgcctt
     7381 atccgcctta aggtatgagg ggggttgtag gcatgataag acgcgccagc gtcgcatcag
     7441 gcgttgagca ccattgccgg atgcggcgta aacgccttat ccggcctaca aaaaacgtgc
     7501 aaattcaaaa tgttgaaata tatccttctg gcctgttttg cgtttgtttc tgtctcaaga
     7561 cgggttactg ccccaaacta ctgcaatcct ggtttgggta aaatttccct gccgtatcgg
     7621 gtgctaatac tttggttggc gcaccggtat ccggattagc ctcaagcgta aagtgacctt
     7681 ctactgacag taacaccggt ttttcactgt ggccgcgcgc agccaggtag ccacgctcca
     7741 gctctgcgtt attcgctacc atgaaacgtt ttccggtcgc gcaatcagtg aaggtcgccg
     7801 catcagccat ataaaaatac atgccccgca gggtcatcgg cgtcatgggt aaactggatt
     7861 gtgccggttc cagcgtatag ttgaactgcg attcaatcgg attgccttca cgatcgagca
     7921 tctccagcgc atcgcctttc gcccgataat atgacttttc acctttgctg tcggttaata
     7981 ccagcttgtc agcggttcgc gcccatgtac cgtaggaagc gaaggaggaa ggttcttcac
     8041 gagcaccgag ataacgctca ttcatcaccc atgttccgtc tttttcgagg aacagagagg
     8101 tttcgattcc ttcgcaatcg gcacacggca gcacgccgcg ccaactttgc ggcatcggtt
     8161 tcaattcggc agcctgcgcc ggagaaagcg tatcgacttc ggcccgatta ttacaaccca
     8221 tcagagtaaa gaggctgatt acagccatcg ctgtcactat cgcttttttc accatccatt
     8281 ccttcttttt attcccgacc gctgcgcatc gccttcacgc ttgatttttg tgctttcgat
     8341 gccagcctgc gctctttcga tgcacgggtg ggccgcgtgg gtcgtcgggc tttttgttct
     8401 gttgttaaat ctttaatcac tgccaccagc cgggccagcg ccgcttcgcg gttcagttcc
     8461 tgactgcggt attcctgtgc cttaatgaca attacgccat cactgctgat caaatgatgg
     8521 ctggcggcga gcagacgctc tttgtaatac tctggcaggc tggatgcccg aatgtcaaaa
     8581 cgcagatgaa tggccgttga ggtcttatta acatgctgcc cgcccgcgcc ctgcgcacga
     8641 atggcggtga tctcaagctc accatcggga atagcaacat gtcgggaaat cacaatcatg
     8701 aaggttgttg ccaggcggtt aagttcactt ccagattatt cttatcatcc gataaccaga
     8761 tcacgccatc ctgaatcgtt gcctgcagcg tcatggtacg atcggcaaag gcgctcactt
     8821 tcgccagctg ctcgtcgtcc agataccaga cggaaagatt ggcaaactgc gcacatttgc
     8881 tctgattttg ctgccaccag atttgcgccg cccgactatt ataggtaaac agcgccactt
     8941 ctgcggcctg ggtgcaggct ttcttaattc gtcgctcatc cggcagcccc agctcaatcc
     9001 acaaatcaat gcccaggtga tcgttacgca gccacgcttc cggctcatca tcggcacaca
     9061 aaccacgggt aaattgcaga cgttcatcag catatttcag ccacgccagc aggcgcagca
     9121 tcatacgctc ctgggtttct gaagggtggc gcgccagcgt cagagaggca tcgagaaact
     9181 ggttgcggtc gagatcggcc acattaaccg tcgctttata aattgtcgct ttaagcgcca
     9241 taagaaaact cctttcgaat caggaggcca ttgtaacgga atacgtgcag ttgcggcgct
     9301 gatattgcag aaatgagtgt ggtatagtca ccttgcgaaa cagagtttat cttcgtaggc
     9361 ttagacttgc atccacggtt aagtcagagt gctgacagga gggcatgtgg aaaaatattg
     9421 tgagttaata cgcaagcggt acgcggaaat cgccagcgga gacttaggat acgttccgga
     9481 cgcgctgggc tgcgttttga aagtgctgaa tgaaatggct gccgacgacg ccctttctga
     9541 ggctgtcagg gaaaaagcag catacgctgc ggcaaactta ctggtgagcg attatgtcaa
     9601 tgaatgatac gtatcaacca atcaattgtg atgattacga taatcttgag ctcgcctgcc
     9661 agcatcattt aatgctgaca cttgagctga aagatggcga aaaattgcag gccaaagcca
     9721 gcgatttagt ctcccgcaaa aatgtggagt atctggtcgt cgaggccgct ggcgaaaccc
     9781 gtgagctacg tctggataaa attaccagct ttagccaccc ggaaatcggt acggtggtgg
     9841 tgagcgagtc ctgattgact ttcgccggat gcgacgctta atgcgtcata tccggccttc
     9901 actaaactaa gcgtttcgct gccagacaaa acttatgcca ttctcacctt cagccacacc
     9961 ttcttgcgtc acaaatcccc ctgccgccgc aatcagcgtt tcgccataaa acagcagtgg
    10021 cgtggtgtca cgtagccacg gcggcacgcc cagctcttgc cagattttct ttagcttacg
    10081 tccgccgtta cgcccgacaa tatgcagcaa tcctggcgct ttgaaacgca cgctgaccgc
    10141 ttcgtctgca cgcggagggc gaatatcgcc tcccgcatta agctgtacac ttccctgccc
    10201 cgccggtaat tccagcggtt gaagccatgt ctgccacggc acaatgtttt cgctttgccc
    10261 ggtgacggat ttaatccacc acagttgcga ctgatagcgt cggatttcaa acgcgcccaa
    10321 acgtaaacag ggggaggcat cttcccgcgc cagcgccact tcctgccaga tcctcaccaa
    10381 cgcgtcgcgg gaaggcatcg gtgcattctg ccctgccagc cagcggcgga taatcgccgc
    10441 gcggcgggca tcactcatcg ccagcattgg cacaatctgc agcgtcccct gcggcgattg
    10501 acagtgtgct aaatcatctg ccagcagttc atccagcagg ctctcttgtt cagcacaaag
    10561 tgcggcgctg cgggccgttg tttcggcaaa atgcggccaa cgctgctgca ataacggcac
    10621 tacgcgcagg cgcagaaagt tacgatcgta tgagtcgtcc tgattacttt cgtcttcaat
    10681 ccagcgtaaa ccatgcgcca atgcccactg cgccagttcc ccccgcgtgc gggcaagcaa
    10741 cgggcggata agccgtgttc cggcaaactc cgaaacttcc gccatagccg aaagcccggc
    10801 agggccgcta ccgcgtttta gcgccagcag aaaagtttca cattgatcgt cgagatgttg
    10861 cgcggtgacc agcacttcac cgggcaacaa ggtgcgggca aatgcctgat aacgcgcctg
    10921 ccgcgcctgg gcctcaatcc ccagcccctc ttgcgcaagt tgtacgcgtt cgaccaccag
    10981 cggcacctgc cactgttggc agacgttttc gcaatgcgta acccaggcat cggcattggc
    11041 acttaaaccg tgatgcacat gaatagcgcg cagagcgccc cccggatttt ccgtccgcca
    11101 ctgcaccaac tgatgcagca gaacggtgga gtcaagcccg ccgctaaagg ccaccagaat
    11161 ctggcgtgag gtgagaagtt gtctattgag cgtgagtgtc atgatggtgc gattttacag
    11221 tagcaatgcc cggcacgtta ccgggcaaat gcggcgatga caagccttac tgctcataca
    11281 gttccaacgg cagcccgtcc ggatcgttaa agaaggtgaa gcgtttttgc gtgtatggat
    11341 cgacacggat ggcttcacac ttcacgttat ggctttcaag gtgcgccact gccgcatcga
    11401 tgtcatcaac gctaaaagcc agatgacgca ggccgcaagc ttccggtcgg ctggggcgct
    11461 ccggcgggaa cggaaatgag aaaagctcaa tcacatattg cccattaagc gccaaatccc
    11521 ctttccacga gtcgcgcgct tcgcgataga cttcgctttg cagcgtgaaa ccaagaatat
    11581 cgcagtagaa agctttgctc accgcataat ccgtcgcaat aatcgcaata tggtgaacct
    11641 gttttaaacc cagcataacg tctcctttat ttgttaacag cacgttactc gcccggaagc
    11701 cgctctggca agttatcccg ccatttttag gactcgtacg cggtaaacgc cgtcttcgtc
    11761 ctgtttcgca ccgtgaatat ccgtttcaaa accggggtaa tgttgcccta cggaacaaag
    11821 catcagtaga aaatcgagta ctgtgcggct ctctttggtc agcatttctc caggcatcaa
    11881 cagcggtacg cccggtggat aaggcaggat catatttgcc gatactctac cgaccagttg
    11941 ttccagcgca atggtttcta cttcaccttt aatctgtctc tgccatgcct gatgtggcgt
    12001 catgatcatc tccggcaaag tatcgaatgc ccgcaacatc aaaccgggaa gatcgtgttt
    12061 acgaatcagc ttatggatcc cttgcgccag atcctgaata cgcatattgc ggtagaaatc
    12121 gggatcttct gcgtacagat ccggaagcat atttttgatt cgcagattga gatcgtaaga
    12181 gcgtttaaat tccgtcaacc cacgcaataa tcccattgct ttggttttat cgatgccaat
    12241 actaaagaga aacagcaggt tatatgggcc ggttttctct accacgatcc cacgctcatc
    12301 gaggaacttc gccaccagcg ccgccgggat cccctcctcg ctcatattgc cctgctcgtc
    12361 catccccggc gtcagaatag tgactttaac cggatcgagg aacatatgat cggcatccgc
    12421 atcgttaaag ccgtgccatt gttcgccagg cgcaacgggc cagcattcgg cttcatccac
    12481 ctgcggcggt tgccagatat cgaaaaacca accgtcagac tcttcccgca gccgctggac
    12541 ctctttgcga aaatgcagag ctcgttctac tgaacggtta atcagccgtt tgcccggatt
    12601 accacgcagc atcgctgctg ccgtctcaac cgaagcaaca atgggataac tgggcgaggt
    12661 ggtggtatgc atcataaagg cttcgttaaa cgcctcttcg tcatactcac ctttaatgtg
    12721 gatcagcgaa gcctgcgata acgccgccag cattttgtgg gtcgattgcg tttcgaagat
    12781 cacttttccc gcaacacgct cgccgctcat accacttttc ccctggtaga tcggatgaaa
    12841 atgggtgtac ggcacccagg cggaatcgaa gtggatcgac gggacatcca gcgtctgttt
    12901 gatccagtcg gtgttgtaga gcaagccatc ataggttgag ttggtaatca ccgcatgaac
    12961 cggccattgt gcctgcgtgg tggcagcgac tttctcttcg atgctgtcgc gagtaaattc
    13021 acggcgcggg atcccaccca gaatccccaa cgcattacgc gtcggtttca gccagactgg
    13081 cactacatcg ttcatcatca acagatgcgc cagcgattta tgacaattgc ggtcaatcaa
    13141 cagcgtactg ccggatggcg cggcgtacat acccacaatt ttgttcgacg tcgatgttcc
    13201 gttggtaacg atataactct gttccgcgcc gaaagtccgc gcgatgtact cttccgcttc
    13261 caggtgtggc ccggtgtggt cgagcaacga accaagctcg gtgaccgaaa tagagacatc
    13321 agccttaagg gtattcccgc cgaaaaagtc ataaaacaga cagccaaccg ggcttttttg
    13381 atatgcggtg ccgcccatat gacccggcgt acaaaaggtg tacttccgct ctttgacgta
    13441 ggtaaacaag gctttcgtga acggtggggt aatgttatca agatattcgt cggtgtactg
    13501 acgcatacga atggcgatat cttccgcctg ccccagcgca tattcaaaaa aacagagcgc
    13561 catccgcata tcctgcacgc tgacatccat cgtcgagtgg gtgttgatga aggcataaag
    13621 cgggagatat tcattaagct gattgatatc gctacataaa tcgagactgt attcatccca
    13681 gtcaaaaatc acgccgcaaa ttcgagggtt atgttcgata aatttcagca aatcaacgct
    13741 gttttgtggc cagataatct gaaagccttg cgccaccagc gccgactcca gttctttgat
    13801 gggctcatct ttataaaaga cgccatgcgg tcccataatg gcaatgatgt tcatgtgttc
    13861 ctcctggaaa atccttcctt aatcatagcc tgctcaaacc gtggcgataa aaaagggcca
    13921 ccgaagtgac cctttttcag aacttttgcg aattacgcgt aaccgtagct catcaggcgc
    13981 tgataacgac gattttttaa atcttcagtg cttaacacgt cgagatcggc cagatccgcc
    14041 agcagttgcg ctttcaacga tgccgccatc gcttccgggt tacggtgagc gccgcccagc
    14101 ggttccggga tgatggagtc gatcagtttc agctctttca gacgcggagc gatgataccc
    14161 atcgcttcag ccgccagcgg cgctttgtcg gcgctcttcc acagaatgga cgcacaacct
    14221 tccggcgaga taacggaata ggtgctgtat tgcagcatat tcactttatc gcccacgcca
    14281 atcgccagcg caccgccaga accaccttca ccgataaccg tacaaactac cggtacgccg
    14341 aggcgagaca tttcacgcag gttgcgtgca atggcttcag actgaccacg ttcttctgcg
    14401 cccacgcccg gataagcccc cggggtgtcg ataaaggtga tgataggcat cttaaagcgt
    14461 tcagccattt gcatcagacg cagtgctttg cggtaacctt ctggcgctgg cataccaaag
    14521 ttacggcgaa ttttttcttt ggtttcacga cctttttgat gaccaatgat catcaccgga
    14581 cgaccatcga gacgggcgat accaccgacg atagctttat cgtctgcata cgcgcggtcg
    14641 ccagccagtt cgtcaaattc atcaaatgcc aggcgaacgt aatccagggt ataaggacgc
    14701 tgtggatggc gtgccagttg cgcaatctgc catgcaccga gatcggcgaa gattttacgc
    14761 gtcagttcta cgcttttttc acgcagacga tgcacttctt catcgatgtt aatatccagt
    14821 ttctcatcct gacggctaac cgcagtcaga gaatcgattt tcgcttccag ctctgcaatc
    14881 ggctgttcaa aatcaaggaa attcagactc atagtattcc tgtattagtc aaactccagt
    14941 tccacctgct ccgaaccaat gaggccacgg agatcgttta ataaacgatc gctcggagag
    15001 acacgccacg tcgcgccaaa acgcaaccgc gcgcgtgcat ccgccctctg atagtagaga
    15061 tgtactggaa ttgtcccaga gcggtggggt tccagagact gacggagtcg gtttaaaagc
    15121 tggtcatcaa tttgcctgtc cgtcagcgag atagcaagcc cgcgagcata tttttcccgg
    15181 gcttcgtcaa tatccatcac ttcgcgagcg gtcattttaa gcccaccgct gaagtcatca
    15241 aagctgacct gtccgctgac gataagtatg cggtcttttt ccagcaaatg ctggtattta
    15301 tccagggcgt cagtaaacaa catcacttcc agccgcccgg aacggtcatc cagcgtgcag
    15361 ataccgatac gattgccgcg cttggtgacc ataacccgcg cggcaacaac gagccccgca
    15421 gccgtgatga ctttaccacg ttctgtcggg tgcatgtctt tcagccttac gcctccgaca
    15481 taacgctcaa tctcttttaa atactggttg atagggtgtc cggtcaggta caggcctaac
    15541 gtttcacgtt ccccatctaa taccacctgc tccggccacg gttggcagct ggcataggat
    15601 tgttcaattt gttccggctc ttcggccagc acgccgaaca tatcggcctg accgatagct
    15661 tccgctttcg cgtgttgatc tgccgctttt aacgcatcgc ccagcgagtt catcagcgcc
    15721 gcgcgatgtg gcccaagacg gtcaaacgcc ccggacatga tcagtttttc cagcacgcga
    15781 cggttcaact ttttggtgtc ggttcgggcg cagagatcaa acagttcgcg gaagtagccg
    15841 cctttattac gggcttcgat gatcgcctca atcggacctt cacctacccc tttgatcgcg
    15901 ccaataccat acacgatttc gccgtcgtcg ttgacgtgga aatggtaaag accggagttt
    15961 atatctggtg gcaggatttt cagccccatc cgccagcact catccaccag gcccaccacc
    16021 ttctcggtgt tgtccatatc ggcggtcatt accgccgcca taaactctgc cggatagtgc
    16081 gctttcaacc ataatgtttg atatgacacc aaagcatagg ccgcagagtg cgatttgtta
    16141 aatccgtaac cagcgaattt ctccaccagg tcgaagattt tcatcgccag ttcggcgttg
    16201 attccgttct tttctgcacc ttcagcaaat acagaacgtt gcttagccat ctcttccggc
    16261 tttttcttac ccatcgcacg acgcagcata tccgcgccac cgagggtata accagaaagc
    16321 acctgcgcaa tctgcatgac ctgttcctga tacaggataa tgccgtaggt tggctccagt
    16381 accggtttca ggctttcatg ctgccactgc acgtccggat aggagatctc ttcgcgacca
    16441 tgtttacggt cgataaagtt atccaccatc cctgattgca acggaccagg gcggaacagt
    16501 gccaccagtg cgatcatatc ttcgaagcag tcaggttgca gacgcttgat caggtccttc
    16561 atgccgcgcg attcaagctg gaataccgcc gtggtttccg agcgttgcag catgtcgaag
    16621 cttttcttgt catccagcgg gatcgcggcg atatccagcg gcggctcgcc attcttcgcc
    16681 cgccgcttgt tgatcatctc cagcgcccag ttgatgatgg tgagcgtacg caaaccaagg
    16741 aagtcgaact tcaccagtcc ggcgtattca acgtcgcttt tatcaaactg ggtgaccgga
    16801 tgtttgccct cttcatcgca ataaagcggc gcaaaatcgg taattttggt cggcgcgata
    16861 accaccccac cggcgtgctt accggcgtta cgggtgaccc cttccagttt gcgcgccatg
    16921 tcgatcaacg ccttaacttc ttcatccgct tcgtagattt ccggcagctg cggctcggct
    16981 tcaaacgctt tcgccagcgt catccccgga tcgggcggga tcagtttcga gatacgatcg
    17041 acaaagccgt acggatgccc cagcacgcgg cctacgtcgc ggatcaccgc tttcgccgcc
    17101 atcgtaccga aggtgataat ctgcgatacc gcatcacgac cgtacatgtc cgctacgtgt
    17161 tcgataacct gatcgcgttt ctccatacag aagtcaacgt cgaagtcagg catggagaca
    17221 cgttccgggt taaggaaacg ttcgaacagc aggtcaaatt ccagcggatc gaggtcggtg
    17281 attttcagcg cgtaggccac cagtgaaccc gcaccggagc cacggcctgg ccctaccggt
    17341 acgccgttat ctttcgacca ctggataaat tccataacga tgaggaagta gcccgggaag
    17401 cccatctggt tgataacctg aagttcagtc tccagacgtt cgtcatattc cgggcggcgc
    17461 ttaacacgtt cttcctcatc agggaataaa aaggccagac gctcttccag gccctctttt
    17521 gcacgcttga ccagataatc ttcggtgctc atgtccccgg tcgggaactg cggcaggaag
    17581 tattcaccaa gacgcacagt tacgttacag cgtttggcga tctcaacggt gttggcaagg
    17641 gcttctggga tgtcggcaaa cagctcgcac atctcctctt cgctacgcat atattgctgc
    17701 ggcgaatagt tacgcgggcg tttaggatcg tcgagggtaa agccgtcgtg gatcgcgacg
    17761 cggatttcgt gtgcgtcaaa gtcgctgctg tcgataaagc gcacatcgtt agtcgccacg
    17821 acgggcaaac cgcgcgcttc cgccagttcc accgccgcgt gcagatagct ttcttcatcc
    17881 ggcctgccgg tgcggatcag ctcgagaaaa tagcgatccg ggaagtgttc ttcataaaac
    17941 gcgacacact catctaccag cgcgctgtta ccacgcaaaa gactgcgtcc gacgtcgccc
    18001 atgcgcccgc cggaaagaag gatcaaccct tcgtttaatt cgataagcca gtcgcgatcg
    18061 atgatcggcc cggcggcacc gtacccgcgc tgatacgctt ttgagatcag caacgtcaga
    18121 ttctgatagc cggtattgtt cgccgccagt accgtcaggt gggttaactc atcacccagc
    18181 aggtcgcact ggacgttaaa atctgccccg acgataggct taatccctgc gccatgtccc
    18241 gctccgtaga acttcaccag accgcaaagg ttggtgaaat cggtgatcgc cagtgctggc
    18301 atacccaacg ccgccgcctt ttttaccagc ggtgcggttt tggccaggcc atcgatcatc
    18361 gagtagtcgc tgtgcacccg caggtgtacg aaacgtggtt cagacatctt cagattccgg
    18421 tttacttaat ctcgacacaa gaatcaggac gcaagtccca gtgcgcgttt gacaggccca
    18481 aagctgcgcc gatggtgttc ggtcgcgccg tgttcagcca gtttttccag atgaaaagcg
    18541 gttggatacc ctttgtgttg ggcaaaacca tattgcggga aaacaatatc cagcgccgcc
    18601 atttcggcgt cacgcgtcac tttcgccagg atagacgcgg cactgatttc cggtacgcgg
    18661 ctatcgcctt tcaccacagc catcgcaggc atcggtaatt tcgggcagcg gttaccatca
    18721 atcaacacat attccggcgc aatatgcagc ccagcgacgg ctcgctgcat cgccagcatg
    18781 gtcgcatgta aaatattcag ttcgtcgatt tcgtgaggtt ccgcgcggcc cagactccag
    18841 ctcaacgctt tctctttgat ctcttcacag agcgccagac gacgtttttc gctcagcttt
    18901 ttggaatcat tcagcccggc aatcgggcgc gccgggtcga gaatcaccgc agcggtgacg
    18961 accgcgccaa ctaacggccc gcgtccgact tcatccacac ccgcaaccag ctgcgtgtgc
    19021 ggatagacaa attcgatcat tgtgctaact ccagaacggc ttgtgccgcc tgctcatcgg
    19081 cattgcagcg gatctgctga tgcagttcac ggaaggtatc gtgcatcgcg tggctggttt
    19141 tcccattcgc caacagcggt aacagcgccg cagccagttt ttgcggctca cactcttcct
    19201 gcaataactc tttgactaac tctctgcccg ccagcagatt tggcagcgag acataatcag
    19261 ttttcaccag ccgcttcgcc aaccagaagg taaaaggctt catgcgatat cccaccacca
    19321 tcgggcattt cgccagcata cactccaggg ctgccgtccc cgacgccagt agtgccgcat
    19381 cgctggcgac catcgcctca cggcccattc catccagcaa atgaaccgac aggtctggcg
    19441 cgactgcagc tttgatgcgt tcaaactgct cgcggcgttt ggcattcacc agtggcacca
    19501 cgatttcgag atccggatat gtctggcgca aaagctgggc cgttttcagg aaatcggcac
    19561 taagcatttc gacttctgca ccacggctgc ccggcaacaa tgccagacag tgggcatcgt
    19621 aagggatccc cagcacatca cgggcactat ttttatctgg atctaatggc atggcatcag
    19681 ccatggtatg accgataaag cggcacggta cgttgtattt gtcataaaac gctttttcga
    19741 aaggcagaaa tgcgagcacc agatcggtgg ctctgcctat tttgaaaacg cgtttctgtc
    19801 gccacgccca gacggacgga ctgacataat gaatggtttt gataccctgc tttttaaggt
    19861 taccttcgag gataatattg aagtcaggcg catcaatgcc aacaaaaaca tctggcttta
    19921 gttcaccaaa acgctttgtc agatcggcac gaatatgcag taagcgacgc agacgaccga
    19981 gcacttcaac aatgcccatc accgccaact cttccatttc gtaccaggct tcgcagcctt
    20041 cagcctgcat tcgtggcccg gcaacaccaa caaagcgggc gttgggcaca cgttctttca
    20101 gagcgcggat taaaccggcc cccaggatat cgccggaggt ttctccggcg accagggcaa
    20161 tcgttaatgg acgctgttca gtcattaacg aatcagaccg cgcgttgagc gtgcaaagaa
    20221 atcggtaaag gctttcactt ccggatatgt ttccgccagt tcagcaattt ccggtttcac
    20281 ttcatcgagc gttttaccgc tacgataaat cagcttatac gcattgcgga tagcggtaat
    20341 cgcctcacgg ctgaatccgc ggcgcttcag cccttcgata ttgacaccga acggcgtggc
    20401 gtggttaccc tgcgcaatga cataaggagg gacgtcctgc gccacaccgg agcagccgcc
    20461 aaccatcaca tgcgcaccaa tgatgcagaa ctgatggact gcggccatgc cgccgatgat
    20521 cgcgaagtcg tcaaccgata cgtgacccgc cagcgttgcg ttgttggcga gaatacagcg
    20581 gttacctacc gtacaatcgt gcgcaatgtg cgcgttgatc atcagtaagt tgtcgctgcc
    20641 caccttcgtc aatccaccgc cctggactgt gccacgatga atggtgacgc tttcgcgaat
    20701 gcggttacga tcgccgattt ccacgcgggt cggttcgcca gcatatttca gatcctggtt
    20761 aacttcgccg atggaggcga actgataaat ctcattatcg cggccaattt tagtatgacc
    20821 attcacgaca acgtgagatt tcagtacggt accctcacca atttcgacat ggggtccaac
    20881 gatacaaaaa ggaccaatgt gtgcgttcgc gccaatcgac gcgccctctt ccacaatggc
    20941 ggttggatgc acaaaggcgg atttatcaat cacgtatcag gcctcccggc tacgagcaca
    21001 catcatcgtt gcttcgcaaa ctactttacc gtcgaccaga gcaacccctt taaaacgggt
    21061 caggccgcgg cgcgtttttt cgaaagtgac ttccatgatc atttgatcgc caggcacgac
    21121 cgggcgcttg aagcgcgctt cgtcaatacc agcgaagtag tacagctcac ccggttccag
    21181 ttttcctacg cttttaaacg ccagaatacc tgttgcctgt gccattgctt ccagaatcag
    21241 cacacccggg aaaatcggtt ttccagggaa atggccctgg aagaatggct cattgacaga
    21301 gacatttttt actgcgcgca gaaaacgacc ttcttcaaaa tccagcacgc gatccaccag
    21361 taagaacggg aaacggtgcg gcagaagttc taaaatctct tcaatctgca gagtatgagt
    21421 gttagtagtc aagatactct tcctgtcaaa atataagaaa cgacaataat aacacggcct
    21481 gccgcaatcg taagaatgag acaggccgta aagtttggcg aacaaaagat ggaacgttag
    21541 tcttgttgat taaccttgcg ctcaagcgat ttcagacgct tgctcatgtc atcaatgttc
    21601 atcaccagtg cagcggtttt gcgccagact ttgttgggtt gcagcggaat gcctgaggaa
    21661 tagacgcctg gttcagtgat gggacgcatc accataccca tgcccgtaac cgtcactttg
    21721 tcgcatattt ccatatgccc gttgattacg ctggctccgc cgatcatgca gtaacgacca
    21781 attttcaggc tgcccgccat aatgacgcca ccggcaaccg ccgtattgtc gccaatcacg
    21841 acgttatgtg caatctggca ctggttatca atgatcacgc cattgccaat aacagtgtca
    21901 tccagcgcgc cgcgatcgat ggtagtgcag gcaccgatct ccacgcgatc gccaataatt
    21961 acgcgaccaa tctgtgggat cttcacccag ttaccacgat cgttggcata accaaagccg
    22021 tctgcgccta ccactgttcc ggactggatc aggcaattct gaccgatctg gatctcatgg
    22081 taaatggtta cgttcgccca gagacgcgaa cctgcaccga ttttgctgtt tttacctacg
    22141 aagcaaccgg caccgataat cacgttatcg cccagttcaa cgccggattc aatcaccgcg
    22201 ttagcgccaa tcgatacgtt gttacccagc ttcgccgtcg cgtcgatcac cgcactgggt
    22261 gcaatgttct gcgcgggctg cggcgtggta tctaaaattt gcgccatgcg cgcgtaagtc
    22321 aggtagggat tcttcactac cagcgcggca cttttcgcga aaggaagatc gtcctgggtc
    22381 atgacaaccg cggacgcctg gcacaagcct aaatgctcac ggtattttgg gttaaccatg
    22441 aacgtaatgt gacctgtttg tgcagattgc atggacgcaa cgccggtgat gacgatatcg
    22501 ccatcaccgt gtagttctgc atccaactgc tgcgctaaat cagccagtcg aattgaaggc
    22561 attacttatt taacctgttt cagtacgtcg gcagtgatgt cttttacatc gctgctgttg
    22621 taagcaacgg cgtttgcatc aacaaccaga tcgatatcct ggctgttggc aacggatttc
    22681 acagcagtct ggatacgagt aaccagtttg ccgcgttctt cattggaacg acgtgcgcga
    22741 tcctgctcaa aagcctgcgc tttctgagca aaagtctggc gctgagccat cacgtctttt
    22801 tccagcttag tgcgatcgct gcccgctttc atggactgca gctttttcat tttagcctgc
    22861 agatcggttt ccatacgctg cagttcgctg gcacggcctt tgaactcatt ttccagcgtg
    22921 ttagaaacac cggttttctg cgctacctgc tggaacaggc tgcccatgtt gacgattgca
    22981 attttgtcag ccgcctgagc agatgttgcc agtgctaaac cgagacctgc agctaataac
    23041 cactttttca caataaactc cttaccatcc catttgcacc ggagggtgca gttctttgcg
    23101 tggcccggcg atttatattg atcgcctaaa gtcatcgcta cactaccact acattccttt
    23161 gtggagaaca cttaccaggt tttaccgatg ttaaactgga actgttctgc cttgtctcca
    23221 tcgtactttt tgaacggctg ggcgtaggag aacaccaacg gccccaatgg ggacatccat
    23281 tgtaatgcga tacccgcaga catacggata ttgcttggat cactatagtc cggatatcca
    23341 gaatattggc tggaatccca gtttgtatcc caaacggtac ccatatccca gaagaaggaa
    23401 gtacgaaccg agttagcata cttatcgcta ataaacggcg tcggggtgat gaactcgagg
    23461 ctggcaaccg ccatggcgtt accgcctaca gcatcatccg atttacacag gtctttcgcg
    23521 ccgtcctgag tcgcacattc gtaatcatag tccggatcat aattactggc ctgatgcggg
    23581 aagtaaactg ctttcggacc aattgtattg gactggaagc cacgcacggt gctggaacca
    23641 ccggcataga agttctcgta gaacggcatc tctttgccgc ctaaaccatc accataaccc
    23701 cagcgggtac gccccagaac aacccatttg tgatcgtcat cgatcggcac ataagtcgcc
    23761 gtgtctaacg tcactttgta gtattcgtta tccgatccag gaatggtcac tttaccggtc
    23821 aggttgacac gtgaaccatc tgtcgggaag taaccacggt caagcttgtt ataggtccaa
    23881 ccatagttga acgtgaagtc gtccgttttg aagctgttat cctgatcaga ggtgctcgga
    23941 tgttcaccca tagagtacag ataacgccac atcgcaacct gaggctgcat gttggacagg
    24001 gagttatgta cataacccag acctgcacgc agcgagttat attcgttaat cgggaagccc
    24061 aacgtcacgt ctgtaccata actcttgttg gtatagtcgg acaggtcggc gtcatctgcc
    24121 tggaagtcat tatagaagag acgaccaccg aggcttacgc catctacggt gaagtacggg
    24181 ttggttaccg acagttcagc ataggtctgg taatcgtttt tggtcccgtt gataccaaca
    24241 gcataacctg tacctaacca gttatcctgt tgcacgccag cctggaagct cacgccactt
    24301 tcagtaccgt aaccaatccc gaagttgaag ctaccagtgt tacgttcttt caccttgtag
    24361 actacatcta cctggtccgg gctacccgga acacgttggg tatcggtatc gacagtttca
    24421 aagaagccca gacgattcag acgctcctta ccctgatcga ccagatcgct tcccagccat
    24481 gcaccttcca tctgacgcat ttcgcgacgc aggacggcat ctttcgaggt atcgttacct
    24541 tcaaaacgga tcttacgcac gtagaaacgg ttacccgcat caacgttcac gcgtaattta
    24601 acggttttgt cggcatcgtt aatttcaggc atcgactgta cgcgcggata ggcataacca
    24661 tagcgaccga gaagcttttt aatgtcatct tccattttgg tcactttggt gccgttatac
    24721 agctcgcccg gctcgatctt agtcagctgc tcaatttcag cggagtgccc ggcaaggttg
    24781 ccgctcactt caacgccaga aagcttgtac tgatcgcctt cggtgatgtt caccgtgacg
    24841 taaatacctt ttttatctgg cgtcagactg acctgggtag agtctatgtt gaaacgggca
    24901 taaccgcggt ccagatagta gctgcgcagg gtttcaaggt cgcccgccag tttctgtttc
    24961 tggtatttac gatcgcctac cacgttccac cacggcactt cgtcacgcag ttggaaatga
    25021 gagatcagtt cgtcggtggt gaaagcatgg ttaccaacaa tgttaatttg ctggatttca
    25081 gctgacacac cttcctggaa caccagtttt aggtcaacac ggttgcgcgg cagcggggtc
    25141 acgacagctt taacgctggc gctatattta ccgacgctgt agtagaagtc ttccagacct
    25201 ttctcgatat cggcaatggt ggtgcgatcg agggattcgc ccacacgcac accagaagcc
    25261 tcgaggtttt gcttcagcat gtcatctttc accgatttgt taccggagaa agtaatgctg
    25321 gcaatggtcg gacgttcttt tacctgaacc agaagggtat caccatcacg aaggacgcga
    25381 acatcctcaa agttgccggt agcaaacaga gcgcgaatgg tattactgat atcttcatca
    25441 ttaaccgtgt cgcctgtgcg caccggcata ctgaggaggg ccgcaccaac ggcgacacgc
    25501 tgaaggcctt cgaaatgaat atctttcact acgaaccctt cagcaccgta tacggtggcg
    25561 ctgctaaaca gcagcgacgc tatgagcaac tttttcatcg ccatcgttat tatgcgttct
    25621 tcctaactaa ctctcataac cgagagaaat cattgaaaag tgcaagcccc attaacagca
    25681 ccagcagaat cgagccaatg cgataacaaa agtcttgaac ccgctcggat accggtccgc
    25741 ctttgatctt ttcgatcgca aggaacagca gatgcccccc gtcaagtacg ggcaacggaa
    25801 acaggttaat tatccctaag ttcacgctaa taagcgcaag aaacggcagg taataaacta
    25861 ccccgagttc cgctgtcatc ccagccccct tggcgataga gatcggccca ctgaggttgt
    25921 tcagtttcac atcaccggtg atcaattttc ccagcatact gaccgtcagc ttcatcagct
    25981 gccacgtttt gtccgtggct tcgacgatgg cgttgaacgg cccatactgg cgtacaactt
    26041 tatactcatc tggcaaagga atgactttcg gctcaatacc gacaaaacca atcgctttac
    26101 cattacccgg tttactctcc gggattaatg tcaaagacaa gggactcccc tgcctttcga
    26161 tttctaacgc taaggattta cccgggttat cccggacaag catcacaaag gtcacccact
    26221 gcgttaaggg ctgaccatcg actttaacga tcctgtcgcc tgcttgcaaa cctgccttgc
    26281 ttgccgccga gtttggctgc acattttcca gtacaggttc aatttgcggc ccacgaggac
    26341 gaatccccag cgaagatacc ggatcttctt tatcaggctc aaacgcccag tgacgtaaat
    26401 cgagctttac atcccgccgt tggtcgctgc caaatggcgc tactgtaatg gtggtgcttt
    26461 catcgccaat tttatcgacc aactgcaaac gcacggcatc ccaatcaggc gtttcgatac
    26521 tatctacggc ttttagttcc gtacctggtg caatttgtgc ttccgcagct atcgaattgg
    26581 ctgctatttc gccaaccacc ggacgtacgc caggcacacc aataataaaa accagccagt
    26641 aggcaaagat agcaaaaatg aagtttgcaa ccggacctgc ggcaataatc gccgctcgtt
    26701 ggccgacaga tttattattg aaggcatggt ggcggagttc cggaacgacc ggttctgcgc
    26761 gctcatccag cattttgaca taaccgccca acgggatcag ggcgataaca tattcggtgc
    26821 cgagcttatc agttcgccgc cagagcgcct taccaaaccc tattgagaaa cgctcaacgc
    26881 gaacaccaca acgccgggca acccagaaat gaccaaattc atgcacggtg ataagtacac
    26941 ccagtgcaac gatgaacgaa gccaaatccc agagaaaact cagcatatta ccttccgtta
    27001 aagcgtcctg aataccagta acaacaagca agcaaagacc ggtaccgcag ccgtcaggct
    27061 atcaatacga tctaaaatac caccgtgtcc tggaattaaa tgaccgctgt ccttaattcc
    27121 tgcttcacgc ttaaacatac tctcggtcag atcgccgagc actgaggcta acgctgcgac
    27181 aatagagcaa atgagtaagg tgacgggagc gacgtcgaga ttcgcccaca tgccataacc
    27241 ccatgagatt accgctgcag tagcgagtcc accgataaag ccttgccagg ttttacccgg
    27301 agaaaccttc ggtgccagct tatgtttacc aaacaatttg ccaaacatat atgcgccgga
    27361 gtcagcgccc cataccagga tcatgacata gagcagccat attgcgccac tgtaatgatt
    27421 ctcgtcatag tgccaggccc gtaacgccag catgccccag aagaagggaa caatggttag
    27481 cacgccaaaa ataaggcgca atgttttaga gttacgccag attgctgcgg aacctgggta
    27541 aaacagcacc agcaatagcg cgacaatcca ccaacccagc gaagcccaaa gtgagatttc
    27601 aaccagcggt tgatgaatat ttcggtgata ttccggcaac agaaaaagca tcagcgccaa
    27661 caataacccg cataacaccg ccaaccatac tcgctgcgaa cgagtggtaa aaccgctaag
    27721 ctgtccccat tcccacgctg ccagcatgca gaccaccagc gttacaatgg cgaaccccac
    27781 cggcggcaac agaaacaacg ccgcgatgac gacgggtatt aacacaaaag cagatatcag
    27841 gcgatacttc agcaaaagcg acccccatca ggctgtttca tcaccgggct cggtgccgcc
    27901 gaaacgacgc tctcgattag caaaggcatt taacgcccct tcaaagtctt gttcatcgaa
    27961 atcgggccag agaacatctg taaagtaaag ttcggcatag gcaatttgcc aaagcaaaaa
    28021 gttactaatg cgatgctccc ccccagtcct aattactaaa tctacagggg ccagttcatg
    28081 catacagaca tgctggttta gcatctcttc atctatctga tctggttgca ggtttccttg
    28141 ctgcaccttt tcagccagtt gcctgactcc ctggactata tcccaacgtc caccgtagtt
    28201 cgccgcaata ttcagcgtca gaccggtatt cccggctgtt agcgcttcag atttacgaat
    28261 acgttcttgc aaacgcgagt taaagcgact ggtatcgcca ataatacgca gacgcacgtt
    28321 atgtcggtgc agacttttta cttcgctatc gagcgcccac acaaacagtt ccattaacgc
    28381 actgacttcc tgcgctggtc ggttccagtt ttcactacta aaggcataca gcgttaacgc
    28441 ctcaataccg ttgttggccg caaaagagac agcccggcgg acggattttg ctccggcttt
    28501 atgcccaaag gcacgaatct tcccttgctt ttttgcccag cgaccattgc cgtccataat
    28561 gatcgcaaca tgacggcagc catgcgctgg caatttttcg ctaagtggtt gagtagcaga
    28621 caacatcacg cgttttttat tccctgacag ggtttacggt actcaggaat actgaagcct
    28681 atacaaaaaa aagccgtgta aaaccacggc atacctgacc ttataaagcc aactacttac
    28741 gatcagatgg cgcagactat atcactgaag ccctacgcta acaaatagcg cgactctctg
    28801 tagccggatt atcctcagct tgcgagacgc atcacctctt ttctggcgac ttcacgcgcg
    28861 ctcgcatcaa cagataacac atcgtccaca cattgtggtt cgcgcatatc cattttttcc
    28921 agtacggata aattcaacgc agcgatatcc gtaaagcgga tttgttgcgc aagaaaagca
    28981 gcaacggtga tttcgtttgc ggcattcaat gctgtcgtcg ctgcctggcc ttgttcgaac
    29041 gcctccatcg ccagtttcag gcatggataa cgatcataat ccggtgcggc aaatgtcaac
    29101 gcacttagtt tgcaaaaatc gagcggcttc acgccagagt tcacgcgatt cggccatgcc
    29161 atggtgtggg caattggcgt acgcatatcc ggttccccca gctgcgccag aacactgccg
    29221 tcctgatagc gcaccattga gtgaatcact gactgcgggt gaatcagcac ttccatctgg
    29281 ctggcgctgg cgttaaacag ccaacgcgct tcaatgtatt ccagaccttt gttcatcatg
    29341 gtagccgaat cgacagaaat tttacgtccc atcgaccagt tcggatgacg gcaggcttga
    29401 tcgggcgtca ttgttgccaa atcgcgcaat ggcgtctcac ggaaagggcc accagacccg
    29461 gtaagtaaaa tggacaccac gccattttgc tcaaggtcag cgtatcccag attatgctgg
    29521 ataggttgcg gtaaactctg aaaaatggcg ttatgttcgc tatcgaccgg taacaattgc
    29581 gctttgctct gctttacggc gtccataaac agacgtccgc aggtaaccag tgattcttta
    29641 ttggccagca aaatggtttt acccgcgcgg atcgcagcaa gcgtaggtaa cagcccagca
    29701 gcgccaacaa tggctgccat cacctgatca acatcctcaa gcgctgccat atcgcaagcg
    29761 gcttgttgcc cacttaagac ttcggtgcgg ctaccctgtt gctgtagcat cgttttaaga
    29821 agtttcgcac tcgcttcatc gtccattacg gcatagcggg gagagaattc caggcactgt
    29881 tctaccatgc gagtgacatt tttgcctgcc accagcgcaa ctacgcggaa gtgttcggga
    29941 ttatggcgca ccacgtccag cgtgctgcaa ccaatcgagc cggtcgagcc cagaatggtg
    30001 agttgcttca tgaaacatcc agagttgaga cagaataaaa agcaaaacgc cgccagccga
    30061 tccgcaagga tctactgagc ggcgtttttg tcgttcaaga aatcagaact gcatcagttc
    30121 tgcttctttg tctgccagcg ccgcttcaat tttcttgatt gcagcatcag tcagtttctg
    30181 tacatcgtcc tgagaacggc gatcgtcgtc ttcgctgatc tctttatctt tcaacagtgc
    30241 tttcactttg tcgttcgcgt cacgacgcac gttacgtact gcaacacgcg cttgttctgc
    30301 ttcaccacga acgattttgg tcagatcttt acgacgttct tccgtcagcg gcggcagcgg
    30361 aacacggatg tcgctacccg cagagttcgg gttcaggcca agatcggacg ccataatcgc
    30421 tttttcaacg gccggagaca ttgaacgatc aaacacgttg attttcagag tacgggaatc
    30481 ttctaccgtt acgcttgcca gctgacgcag cggcgtcggc gtgccgtaat attccacgac
    30541 aatgccatcc agcaggctgg gagaagcacg acccgtgcgt attttgctga tttgggtttt
    30601 gaacgcttct acgcatttgt ccatgcgtac ttcagcatct tttctgatat cgctaatcac
    30661 gttacgaatc cttgaaaact tgtctcagtc agaccagaca gtcacacaca gtggaagtgt
    30721 gctaagtata gccctgatta aacatattac gggtaagatc ccccgcgata cttacgcgga
    30781 atcttaccct tatttatcca tcacgggaat tattccgtga ttaaagtccc ttctttttca
    30841 cccattacca cacggcgcag cgcacccggt ttgttcatgt tgaaaacacg aatcggtaat
    30901 ttatggtcac gagccagcgt gaaggccgcc aggtccatga ctttcagctc tttttccagc
    30961 acttcgctgt aagtcagttg ctcgtacatg gttgcggttg gatctttcgc cggatcagcg
    31021 gtaaacacgc cgtcaacttt ggttgctttc agcaccacat cggcttcaat ttcgatacca
    31081 cgcaggcaag ctgctgagtc ggtggtaaag aacgggttac ctgtaccggc ggagaggatc
    31141 accacacggt tgttgcgcaa caggctgata gcttctgccc agctgtagct gtcgcacacg
    31201 ccattcaatg gaatagcgga catcagacga gcgttcacat aggcgcggtg cagtgcatca
    31261 cgcattgcca ggccgttcat tacggtcgcc agcatcccca tgtggtcgcc cacaacgcgg
    31321 ttcatacccg ctttcgccag accagcgcca cggaacaggt tacccccacc aatcaccaca
    31381 ccaacctgaa tacccagttc aaccagttct ttgatttcct gagccatacg atccagtatg
    31441 cttgcatcaa taccgaagcc ttcagtgccc tgcagagctt cgccactcaa cttaagcaga
    31501 atgcgtttat agacgggttt tgcattggta gccatgtttc tttcctgaga ctgtcaacga
    31561 ttgaaatgaa ttaatactag cgacattgta cgtcgctttt cagcgcagcc ccacaagggt
    31621 tagctgaatt tacaagatgg gcacaaaaag aagccgccct caggcggctc ctttttgata
    31681 attaagactg cttggacatc gcagcaactt ctgctgcaaa gtcagtctca actttctcga
    31741 tgccttcacc cacttcgaag cggatgaagc cagtcacttc agcgttatgc tctttcagca
    31801 gctgaccaac agttttgctt ggttccataa cgaacggctg accggtcaga gaaacttcgc
    31861 cggtgaattt cttcatgcgg ccttcaacca ttttctctgc gatttctttc ggcttaccag
    31921 actgcatcgc gatatccagc tgtacctggt attctttttc taccacttca gcggatacgt
    31981 cttccggttt gatgaattct ggcttgcttg cagcaacgtg catagcgatg tgtttaacca
    32041 gctcttcgtc agcgccttta gcagcaacca gaacgccgat acgcgcacca tgctgataag
    32101 aacccagaac gtcgccttcc agcgcagcaa cgcggcgaat gttgatgttt tcaccaattt
    32161 tcgctaccag cgcaacacgt tcttcttcga actgtgcttt cagaacttca acgtcagtga
    32221 ttttgccagc aacagctgcg tccagaactt tgtctgcgaa cgcctggaaa ccagcgtctt
    32281 ttgcaacgaa gtcagtctgg cagttaactt ccagaatgat gccgtagttg ccgtcgattt
    32341 tggttttgat cacgccgtca gcagcaacgt tgcctgcttt tttcgctgct ttaatagcac
    32401 cggacttacg catgttttcg attgccagct cgatgtcgcc gttagcttca gtcagtgctt
    32461 ttttgcaatc catcatgcct gcgccagtac gctcacgcag ctcttttacc agggatgcgg
    32521 taatttcagc cattctaaaa tcctcggaga tgtgatctgc ccggcattaa accgcacaga
    32581 tataaaagtg aaaaaggggg ccatatgcag gccccctaac caaacgtgta ctacctggtc
    32641 tataagggct ctcccccttc atatttcgcg ccgcagatgc gtcatcttcg ctcgctcatc
    32701 ccggtcactt actgatgtaa gctcccggga attctcgagc ttgccgcctt tctgcaactc
    32761 gaactatttt gggggagtta tcaagcctta ttactcagct tctacgaagc tttcttccgc
    32821 ctgggaagcc agatcctgag aacggccttc acgtacggtt gcagcaacag cgcccaggta
    32881 cagggtcaca gcacggattg cgtcgtcgtt acccgggata acgaagtcaa caccgtccgg
    32941 atcagagttg gtatcaacga tagcaaatac cggaataccc aggttgtttg cttctttgat
    33001 agcaatgtgt tcgtggtcag catcgattac aaacagagcg tccggcagac cgcccatgtc
    33061 tttgataccg cccaggctgt tttccagttt ctccagctca cgagtgcgca tcagcgcttc
    33121 tttcttggtc agcttgtcga aagtaccgtc ctgagactga gtttccaggt ctttcagacg
    33181 tttgatggac tgacgaacgg ttttccagtt agtcagcata ccgcccagcc agcgatggtt
    33241 cacgaagaac tggtcgcagc tcagagcagc gtctttcacc gcttcgcttg cagcgcgttt
    33301 agtaccaacg aaaaggattt tacctttgcg agaagcaatc ttgttcagtt cagccagagc
    33361 ttcgttgaac atcggtacag ttttctcaag gttgatgatg tgaactttgt tacgcgcacc
    33421 gaagatgaac ggcttcattt tcgggttcca gtaacgggtc tggtgaccga agtgaacacc
    33481 agccttgagc atgtcgcgca tggaaacagt tgccatgatt aaaacctcta tataaaagtt
    33541 ggggttatgc ctccacgtat cccatattac cgaccccaaa gggcaccccg gaatatgtgc
    33601 cgatacgtgt gtgttgttac acaaagtgag attgtcgctt ccgtccagtc tgtgtatacg
    33661 aaatggatcg gaagtccggc gcgctttata ccacaaatac gccgtggaca ccaataattg
    33721 ttggcgctgt gtacagcatc agacgtcgaa ttttctatta tagaaaacct tcagtggcac
    33781 gtttggcgaa attcagaatg attctcaatt tgcccgggtg tgataccatt gacggcactt
    33841 acatatatat tgtcggtatc accgacgctg atggacagaa ttaatggcta tctcaatcaa
    33901 gaccccagaa gatatcgaaa aaatgcgcgt cgctggccga ctggctgccg aagtgctgga
    33961 gatgatcgaa ccatatgtta aaccgggcgt cagcaccggc gagctggatc gcatctgtaa
    34021 tgattacatt gttaatgaac aacacgcggt ttcagcctgc ctcggctatc acggttatcc
    34081 gaaatccgtt tgcatttcta ttaatgaagt ggtgtgccac ggtattccgg acgatgctaa
    34141 gctgctgaaa gatggcgata tcgttaacat tgatgtcacc gtaatcaaag atggtttcca
    34201 cggcgatacc tcgaaaatgt ttatcgtcgg taagccgacc atcatgggcg aacgtctgtg
    34261 ccgtatcacg caagaaagcc tgtacctggc gctacgcatg gtaaaaccag gcattaatct
    34321 gcgcgaaatc ggtgcggcga ttcagaaatt tgtcgaagca gaaggcttct ccgtcgttcg
    34381 tgaatattgc ggacacggta ttggtcgcgg cttccatgaa gaaccgcagg tgctgcacta
    34441 tgactcccgt gaaaccaacg tcgtactgaa acctgggatg acgttcacca tcgagccaat
    34501 ggtcaacgcg ggtaaaaaag agatccgcac catgaaagat ggctggacgg taaaaaccaa
    34561 agatcgcagc ttgtctgcac aatatgagca tactattgtg gtgactgata acggctgcga
    34621 aattctgacg ctacgcaagg atgacaccat cccggcgata atctcgcacg acgaataaaa
    34681 tgaagccggc gaatgccggc ttttttaatg cgataattta atcttatggg tggcgcacaa
    34741 tgaataccct tccagaacag tacgcaaaca ccgctctccc caccctgccc ggtcaaccgc
    34801 aaaatccatg cgtctggccc cgtgatgaat taaccgtcgg tgggataaaa gcccatatcg
    34861 atactttcca gcgttggctg ggtgatgcct ttgacaatgg gatctctgca gaacagttga
    34921 ttgaggcgcg caccgagttt atcgaccagc tcctgcaacg attatggatt gaagcgggat
    34981 tcagccagat tgccgacctg gcattggtcg ccgtcggtgg ctacggtcgt ggcgagctgc
    35041 atccactttc tgacatcgat ttactgattt taagccgtaa aaagctcccg gacgatcagg
    35101 cgcaaaaagt gggcgagctg ttaacgctgc tctgggatgt aaagctggaa gtcggtcata
    35161 gtgtgcgcac gcttgaagag tgcatgctgg aagggttatc ggatttaacc gtcgccacca
    35221 atttgatcga atcccgctta ttaattggcg atgttgcgct atttcttgaa ctgcaaaaac
    35281 atattttcag cgaaggattc tggccttccg acaagttcta cgcggcgaaa gttgaagaac
    35341 agaaccagcg ccatcagcgt taccacggca ccagctacaa tcttgaacca gacatcaaaa
    35401 gtagccctgg cggcttgcgc gatatccaca ctctgcaatg ggtggcccgc cgtcattttg
    35461 gcgcaacatc gctggatgaa atggttgggt ttggcttctt aacctcagcg gagcgggcgg
    35521 aattaaacga atgtctgcat atattgtggc gtattcgctt tgccctgcat ctggtcgtca
    35581 gccgttacga taatcgcctg ttattcgatc gccagcttag cgtcgcccag cgtctgaatt
    35641 acagtggcga aggtaacgaa ccggtcgagc ggatgatgaa ggattacttc cgcgtgacac
    35701 gccgcgtcag tgaactcaac cagatgctgc tgcaactgtt cgatgaagcc atcctcgccc
    35761 ttcccgccga cgaaaaacca cgtccaatcg acgatgagtt tcagctacgc ggtacgctaa
    35821 tcgacctgcg tgatgaaaca ctatttatgc gccagccgga agccatcttg cgtatgttct
    35881 acaccatggt gcgcaacagt gcgatcaccg gcatttactc caccacactg cgccagttac
    35941 gccatgcccg tcgccatctg caacaaccgc tgtgtaatat tccgcaagca cgaaaactgt
    36001 ttttgagcat tctacgtcac cccggagcgg tgcggcgcgg gctattgcca atgcatcgcc
    36061 atagcgtact cggcgcgtat atgccgcaat ggtcacatat cgtcgggcag atgcagtttg
    36121 atctgttcca cgcctacacg gtggatgaac atactatccg cgtgatgctg aaactggaga
    36181 gttttgccag tgaagatacg cgccagcgcc atccgttgtg tgtgaacgtc tggccgcgcc
    36241 taccgtcaac tgagctgatt ttcatcgccg cgctgtttca cgatatcgcc aaaggacgcg
    36301 gcggcgacca ctctattctc ggtgctcagg atgtagtgca ttttgccgaa ctccacgggc
    36361 tgaactcacg cgaaacacag ctggtcgcct ggctggttcg ccagcacttg ctgatgtcgg
    36421 tgacagctca gcgtcgcgat attcaggacc cggaagtcat caagcagttt gccgaagaag
    36481 ttcaaacgga aaatcgtctg cgctatctgg tatgcctgac agtggctgac atttgcgcca
    36541 ccaacgaaac gctgtggaat agctggaagc aaagtctgtt gcgcgaactc tatttcgcca
    36601 ccgaaaagca gctacgacgc ggaatgcaaa acacgccgga tatgcgcgaa cgggttcgcc
    36661 atcaccaact ccaggcgctg gcactactgc gcatggataa catcgacgaa gaggtgctgc
    36721 accaaatttg gtcacgctgt cgcgcgaact attttgtccg tcatagccca aatcaactgg
    36781 cctggcatgc gcgccattta ttacagcatg atttaagcaa accgctggta ttgcttagcc
    36841 cgcaggctac gcgtggaggc accgagattt ttatctggag cccggaccgc ccttacttgt
    36901 ttgctgctgt ttgtgccgag ttagatagac gcaacttaag cgttcacgac gcacaaattt
    36961 tcaccactcg cgacggtatg gcgatggata cctttatcgt gctggaaccc gatggtagcc
    37021 cgctatccgc agatcgtcat gaggttattc ggtttggtct ggagcaagta ctgacacaaa
    37081 gtagctggca gccaccacag ccgcgtcgtc aaccggctaa actacgtcac tttactgttg
    37141 aaaccgaagt gacgtttttg ccaacccata ccgaccgcaa atcgttcctt gagcttatcg
    37201 cgctcgacca gccaggactg ctggcgagag tcgggaaaat ttttgccgat ctgggaattt
    37261 cgcttcatgg tgcccgaatt acaaccattg gcgagcgagt agaagattta ttcataattg
    37321 ccaccgctga ccggcgtgca cttaataacg agttgcagca ggaagtgcat cagcggttga
    37381 cagaggccct caatccaaac gataaagggt gatgtgttta ctgatatgaa aagagtttaa
    37441 caatgcagca gttacagaac attattgaaa ccgcttttga acgccgtgcc gagatcacgc
    37501 cagccaatgc agacaccgtt acccgcgaag cggtaaatca ggtgatcgcc ctgctggatt
    37561 ccggcgcact gcgtgtagcg gaaaaaattg acggtcagtg ggtgacgcat cagtggttga
    37621 aaaaagcggt gctgctctct ttccgtatta atgataatca ggtgatcgaa ggggcagaaa
    37681 gccgctactt cgacaaagtg ccgatgaaat tcgccgacta cgacgaagca cgtttccaga
    37741 aagaaggctt ccgcgttgta ccacctgcgg cggtacgtca gggtgcgttt attgcccgta
    37801 acaccgtgct gatgccgtct tacgtcaaca tcggcgcata tgttgatgaa ggcaccatgg
    37861 tcgatacctg ggcgaccgtc ggttcttgtg cgcagattgg taaaaacgtc cacctttccg
    37921 gtggcgtggg catcggcggt gtgctggaac cgctgcaggc taacccaacc atcattgaag
    37981 ataattgctt catcggcgcg cgctctgaag tagttgaagg ggtgattgtc gaagaaggtt
    38041 ccgtcatttc catgggcgta tacattggtc agagcacccg tatttacgac cgtgaaaccg
    38101 gcgacatcca ctacggtcgc gttccggcgg ggtctgtggt tgtttcaggt aatctgccgt
    38161 cgaaagatgg caaatacagc ctctactgtg cggttatcgt taagaaagtt gacgcgaaaa
    38221 ctcgcggcaa agtcggcatt aacgaactgc tgcgtaccat cgactaaaag tatgcacacg
    38281 ggcagcacga cgctgcccga ttgctttctg gttcagaata attcgtccta ctcattcttc
    38341 ctcccccctt ttttaaatac tttttcatct tgtttcggac tgtcatgtaa ttcgcaaaac
    38401 gaagataaat gtgctaatcc atcccatgga atatcgccac aataaagcag gtaatttttt
    38461 gatttttacg ccatgataga agtctggaag aaaaaggctt tctgcgatca cagtcgagcc
    38521 gggcgggcca ggccaaatgc taaaataagc acattttact gactcatttt gaggtaacgc
    38581 tatgtacgac aatctgaaaa gtctgggtat taccaatcct gaagaaattg atcgttacag
    38641 cctccggcag gaagccaaca acgatattct gaaaatctat ttccagaaag acaaaggcga
    38701 gtttttcgcc aagagcgtta agtttaaata tccgcgtcag cgtaaaacgg tcgtcgctga
    38761 tggtgtgggt cagggttata aagaagtcca ggaaatcagc ccgaatctac ggtatatcat
    38821 tgatgagctt gatcaaatct gccagcgtga ccgcagcgaa gttgatctta agcgtaagat
    38881 cctcgacgac ttacgtcacc tggagtcagt cgtaaccaat aagatcagcg agattgaagc
    38941 cgatctggaa aaactaacgc gtaaataatc ccgaattgac ggttaactcg ggcattcccc
    39001 tctccccttc gggctgaggg gaatgagcca gcgccgcctc aaccttaacg cataacctac
    39061 cgctcttcat ccagttgtaa cgccacatac agcagcaacc tgtcatcaaa attgcccaaa
    39121 tcaagcccgg tcagttccga tatacgatta agccgatact ccagggtatt acgatgaata
    39181 aacaacgcct ttgacgttgc cagcggttgc acattgtgac gaaaccacgc cgccagcgtt
    39241 cgtcgcagca agccgttatt gtccatcgct ttcagccgcg ccagcggtcg cgccagttcg
    39301 ttggcctgcc agtcgccacg caaactgtcg agtaacacag gtaacatcag atcctgataa
    39361 aaatagcagc gactttctgg catccgctgt ttacccacca ccatcgtcgt tttcgccgta
    39421 cgataggatc gggcaatact gccaggaccg gtaaaatagt tgcccagtga aacgcgaaaa
    39481 cgcagctggc cgtactcttt catgcgggta atcagttgtt caactcgctt acgatgatct
    39541 tctacatccc agcgcccaaa agagttcaac gccggtttca acaccaccat ttcggttagc
    39601 gagacaatcg ccaccagatt attacgctcg ggcgtagtca gcgcgttttg cagttgttgt
    39661 aactccgcca ttgcgctgtc cacaccaagc tgaccgctgt cgacctcaac aatagccacc
    39721 actcgcggtt gattgagatc gatccccagc cgttgcgccc attcagtaag tgcgggagta
    39781 ttctcctctg cctgaatcag gttcatcacc agttcctccc gcaaacggct atcctgcgcc
    39841 aacaagtgca tcaaccgcga ctgttccagc atcatttcag ccgtcatgca gaccagttcg
    39901 ccatatttac gcagattctc tggttcacct gtcaggccaa ttacgccgac aatttcacct
    39961 tccagccgta acggtagatt aatcccttgc cgcacaccgt gcagatgacg tgccaccgcg
    40021 tcatcgatat cgacgactcg tccctgtgaa agtaccagca atgcaccttc gtgcaattca
    40081 ccaatacgct cacgatcgcc gctgccgata attcgcccac gggcatccat tacgttgata
    40141 ttggtatcga tgatgcgcat ggtacgtgcc acgatatcct gcgccatttt ggtatcaaga
    40201 tgccagccag ccataagtcc tccgttatgc acggcttagc ataaggaagt acgtaacgta
    40261 cggcattgtg caaatgccta aaggatgatg aagatgtatg gagttgtggt gaagttcaca
    40321 gattgtaagg agaccccctt cccgttttca ggaaggggtt gagggagatt actgcattaa
    40381 caggtagatg gtgctgtcgc cgcgctgaat gttcagtgcc agcacagacg gtttgctgtc
    40441 gagaacttta cgcagttcag cgatgttttt cacagcctgc tggttcgcgc caataatcac
    40501 atcacctttc ttcaggccga tctgcgcagc cggagtgccc gttttcacgt tgttcactac
    40561 cacgccctga tctttgcctt tgttgctcat ctcagcgcct tcaatgccgt tgaagatgga
    40621 gctggaatca acctgattct ggctgctctg ctgcagttcc agattcacgt tgacctgctt
    40681 accgtcgcgc agcaagccca gggtcagttt gctgcccacc ggcatagtac ccacttgagc
    40741 acgcagtgcg gcaaagctgc tgatcggctt accgttcagt gaggtgatca catcacccgc
    40801 tttaatgccc gcttttgcag cggaggaatt aggcagaacc tggcttacga aagcaccgcg
    40861 ctgggcgtca actttcatcg ctttcgccag ttcggagttc agctcagtcc ccataatacc
    40921 cagctcaccg cgtttcacct ggccgtattc caccatctgc gaggtcaggt ttttcaccat
    40981 gttactcggg atagcaaaac cgataccgat gttaccgccg tccggtgcta ggatcgcggt
    41041 gttgataccg atcagttcgc cgttcaggtt aaccagcgca ccaccggagt taccacggtt
    41101 gatcgctgca tcggtctgga tgaagttttc gtagttttcg gcattcaggc cgctacgccc
    41161 cagcgcagag acaatcccgg aagttaccgt ctcgcccaga ccaaacgggt taccaatcgc
    41221 tacggtgtaa tcacccacgc gcagtgcatc agaatccgcc atcttaattg cggtcaggtt
    41281 tttcggattc tggatttgga tcagcgcgat atcagagcgc ggatctttgc caaccatctt
    41341 cgcgtcgaac ttacggccat cgctcagttg aactttaatg accgtcgcgt tatcaacaac
    41401 gtggttgttg gtgacgacat agcctttatc ggcatcaatg atgacgccgg aacccagcgc
    41461 catgaatttc tgttgctggc cgccaccatt accgccctgg ccaccctggc agaacggaga
    41521 gctctggaac ggagaacctt cctggcagaa cggagaatca tcaccgaaga actgctggaa
    41581 attacgcggc atacgcggcg tattaacggt tgtgctacct tctacgttaa tgctgaccac
    41641 tgaaggcatc accttttcga gcatcggtgc aaggcttggc atctgctggg ctgtcgttgc
    41701 tgaagaagtc tcagccgccg ttgcagagag cggagataac gccaaaccta aactcagagc
    41761 cagtgcactc agtgctaatg tggttttttt catgtatttc agtctcgatt aacagataac
    41821 gcaaaattgc tgtgttcttc agattcgttt tatagcgcga agttccgaac taaagtttct
    41881 gcaaaaggta aaaatttatt gttcgtcttt acaaaagcct ggttattgtt ctacggccat
    41941 cagacgtcgg tattcatccc aggcatagag gtcggtcata ccgctgatat aatcctgcag
    42001 caggcggcaa cggtaataat attcccatag cggaaactca ggagaatctg acggtaattt
    42061 actgacagcc tcgacatagg ccagccgatg gcgcgtcgag agtttgtgga ataagcgaga
    42121 ttcaataggg gaacgtttca cccgttcttt ttctaccagt tcagtaaagt ctgataatga
    42181 caggcttaat aaagggcgat aaatctctaa taatccgcta atgacccgat agccctgcaa
    42241 ttcaagctgc tcgacatctg gatggctaaa cacatgtttt acagcgacat ttttatatag
    42301 cttaagaaga tcgctgcatt cgctggcatc ttccaataat gcatgattaa acgttccggc
    42361 gaaaatcgca ggcagattat caataaatcg ttgtgccgcg tagggtacca gtttatttag
    42421 ggtgtttacc cgtaaataca taaaaaactg atcttccgta ctgcggctta aactatttga
    42481 gcgtgatttt tcccaggcat tttcaaccac cagcgaaaag agcgaacctt tctcatgctg
    42541 gccccacgcc tcgtgcaaat gatgataaag ctgctcaacg gaaaatattc ttttctctac
    42601 cgcatcttca agatctgcca cacaatagga gatgtcgtcg gcagcttcca taatccacgt
    42661 taatggaaaa cgactgtaaa gcgccaaatt aagttcttta cgcaaccggg caatataggc
    42721 ttcttcagaa agataataac ccggcttttt cattaaatag tgatgtgtct caggcgtttc
    42781 gccacgccac cacgctggac gggtatattt taaaataccg ccaacctgcg cccaggtgag
    42841 attcatccgc atcaatgtat gcaccagacg aatgccttgt gcattcccct caaaatgaca
    42901 taagtcctga cgaatcttgc gccgcagctc gttaagcggt tcttccccgt cccgtaaacg
    42961 tagtaccgcc acgctgcagc gatcgtcagt cagaggctgg ctttcggcat cttccgggtg
    43021 caaacgctgg cgaaaccagt catttatcgc cgcttcgcca aaatgaccaa acggcggatt
    43081 gccgatatcg tgcatcaggc atgacatctc aacaatgctt tcaaaaggtc cggtcagttc
    43141 atccaggccg tatgcttcca gcaatttaag ctctttcaga cggcttaaaa tttctttggc
    43201 gatgtagcgc cccacctgct ggacttccat cgagtgggta agacgcgtgc gcacggcggc
    43261 attgcgctcc agtggaaaaa cctgggtctt ttgttgcaga cgacgaattg ccggagagtt
    43321 gatgatacgc ccgcgatcgc tctcgaagat ccgcaggatc tcatgttcgg ttttaacgcc
    43381 ctgcggtgaa cggtaacgac gatgccagtt tatttttttt cggaaatcaa tctgtgccat
    43441 gtcctctccc gcgtgagaaa tacgcttccc cgtaagcgca tggtaaacta tgccttcaaa
    43501 tcgggcttat cgcgagtaaa tctatgaaaa tcggcatcat tggtgcaatg gaagaagaag
    43561 ttacgctgct gcgtgacaaa atcgaaaacc gtcaaactat cagtctgggc ggttgcgaaa
    43621 tctataccgg ccaactgaat ggaaccgagg ttgcgcttct gaaatcgggc atcggtaaag
    43681 tcgctgcggc gctgggtgcc actctgctgt tggaacactg caagccagat gtgattatta
    43741 acaccggttc tgccggtggc ctggcaccaa cgctgaaagt gggcgatatc gttgtctcgg
    43801 acgaagcgcg ttatcacgac gcggatgtca cggcatttgg ttatgaatac ggtcagttac
    43861 caggctgtcc ggcaggcttt aaagctgacg ataaactgat cgctgccgct gaggcctgca
    43921 ttgccgaact gaatcttaac gctgtacgtg gcctgattgt tagcggcgac gctttcatca
    43981 acggttctgt tggtctggcg aaaatccgcc acaacttccc acaggccatt gctgtagaga
    44041 tggaagcgac ggcaatcgcc catgtctgcc acaatttcaa cgtcccgttt gttgtcgtac
    44101 gcgccatctc cgacgtggcc gatcaacagt ctcatcttag cttcgatgag ttcctggctg
    44161 ttgccgctaa acagtccagc ctgatggttg agtcactagt gcagaaactg gcacatggct
    44221 aagtcactgt tcagggcgct ggtcgccctg tcttttcttg cgccactgtg gctcaacgcc
    44281 gcgccgcgcg tcatcacgct ttctcccgcc aacactgaac ttgcctttgc cgccgggatc
    44341 acgccggttg gggtcagcag ctattccgac tatcctccac aagcgcaaaa gattgagcag
    44401 gtttccacct ggcaggggat gaatctggaa cgcattgtcg cgctgaaacc cgatctggtg
    44461 attgcctggc gtggaggtaa tgccgagcgg caggttgacc agctggcttc gctgggaata
    44521 aaagtgatgt gggtcgatgc gacaaacatt gaacaaattg ccaatgcgtt acgtcaactg
    44581 gctccctgga gtccgcaacc ggataaggcc gaacaagccg cgcaatccct gctggatcaa
    44641 tacgcgcaat tgaaagcgca atatgctgat aaacctaaaa aacgtgtttt tctgcaattc
    44701 ggcattaatc cgccatttac cagtggaaaa gagtcgattc aaaaccaggt actcgaagtt
    44761 tgtggcggag aaaacatctt taaagacagc cgggttccct ggccgcaagt tagccgcgaa
    44821 caggtgttag cacgctcgcc acaggcgatt gtcattacag gcggaccgga ccaaattcct
    44881 aaaatcaaac aatactgggg tgaacagctc aaaattcccg ttattcctct cacgagtgac
    44941 tggtttgaac gtgcaagccc acgtattatc ctcgctgcac aacagctctg taatgcgctt
    45001 tcacaggtag attagcgcct gaccttttta ggtgctcagt ggggattgaa caatgctcgt
    45061 ctattggctg gatatagtcg gcacagcggt atttgccatc tccggcgttt tgttagccgg
    45121 aaaattgcgt atggaccctt ttggtgttct ggtactgggc gtggttaccg ctgtaggcgg
    45181 cgggacaatt cgcgacatgg cgctggatca cggcccggta ttttgggtga aagatcccac
    45241 cgatctggtc gttgcaatgg taaccagcat gctgaccatc gtgctggtgc gccagccaag
    45301 acacttacca aaatggatgt tgccggtgct ggacgccgtt ggtctggcgg tgtttgtcgg
    45361 cattggcgtg aataaagcct ttaatgcgga agccggtccg ttaatcgcgg tttgtatggg
    45421 cgtcattact ggcgttggcg gcgggatcat tcgtgatgtt ctggcccgcg aaatccccat
    45481 gattttacgt acagaaatct acgcaactgc ctgtattatc ggcggtattg tccacgctac
    45541 ggcttattac acattttccg taccactgga aacagccagt atgatgggca tggtcgtgac
    45601 gctattgatt cggctggcgg ctattcgttg gcatcttaag ctaccgacgt ttgcgctgga
    45661 tgagaatggg cgttgaagag agaagatgtg ccggatagtt tatccggcac atgaacaaca
    45721 gattagatac taaaggaaga accgcaaccg caggtgcttt tcgcgttcgg gttggtcacg
    45781 atgaaacgag aaccttccag tccttcggta tagtcaacgg aaccgccgac cagatattgc
    45841 aggctcatcg gatcaaccac caggccaacg ccctgttttt cgatggtcat atcgccttcg
    45901 ttcacctgat catcaaaggt gaaaccatac tggaagccgc tgcaaccgcc accggtgata
    45961 tacacgcgta atttcagatt cgggttatct tcgtcagcga tcaggctttt aactttgttg
    46021 gctgctgcgt cggtaaactc cagcggcagt gctacgtcat cactcatatt ttgctccaaa
    46081 cgacatcggc aattgggcaa attctaaccc aataataatc gccattatct aataccctgg
    46141 tatttcgttc aagtattctc gctggctgat gcggccttgc ttcgcgccag ttgctcagct
    46201 tcctgttttg ccagcgtgcg cgcaagaatc gccgagtata gcggtttccc gccggtaaat
    46261 tgcgctaata gtgttgcgcc aagaccggta ataatcattg gcaaaatgag ctggtagtta
    46321 tcggtcatct ccagaaccag aatgatcccc gttaacggcg cgcgaataga tgccgccagt
    46381 aacgccccca ttccggcaat agcaaacgtc cccgcctcaa ggtgatattg cggaaacagc
    46441 tcaacggcaa ccattccgaa agcggttccc agcacagtac ccagcgccag catcggggca
    46501 aaaataccgc ccggcgcgcc ggaagagaag cagagtaagg tggtaatgac ccgcgcgacg
    46561 aagataaaca ccagcattcc catgctgaaa ttccccgcgg tagcgatagg aatcaggtta
    46621 aaaccgccgc ccgacgttgc tggtgccaca aaccccagca atccacacag accgccaatc
    46681 gcaccaccca tcagtaccca tttggtaata ttgccgccgt gcacacggtg cagcaaatcc
    46741 tgcatcccca gcacccattt attaaaaata gggccgaaaa tgccaaaaat aataccgagg
    46801 atcagataaa gccacagcgt attaagcggc gcgtcagaaa gtttaccgac gtcaatcaac
    46861 gcaacttcat gattaaaaat ccggtacata atggtcgaca taatgacgcc aataaatacc
    46921 gctttaatcg aaattaacgt atagcgaaac tgcggacgca tctcttcgat aataaacaaa
    46981 atacccgcca gcggcgcgtt aaaggccgcc gccagacccg ccgccgcacc ggttgccaac
    47041 agcgtatggc gagcttcatc acctttcagg cggaaaacat cgagtaccat ccgcccgata
    47101 ttaccgccga tctgcacggt tggcccttcg cgccccaaca ccatgcctcc gccgagtgtc
    47161 cccagcccgc caaagaattt caccggcaat acacgccacc agcgaacagg acgttgatct
    47221 tccagcgccc cttcaatttc cgggatcccc gacccccctg cttcgggcgc gtatttgcgc
    47281 accaaaaagt agccgaacat cgccagcacc gccgaacaga gaaaagcaac ggttaacagt
    47341 agcggataat tatcagcagt atgtaccagc gcccccatac gttggttctg caaccaggcg
    47401 acacctttgt caaaagcaac cgctgccagc ccgacaagcg tgccgacgac tgccgccata
    47461 aacaaaatgg ctaacggggt tttatcgcgc tcaagaagtt ggcgaatcag ttgtctgcgt
    47521 cgcaggcgcg cggcctgcgg tgtttctaaa gagggagtat cagttttcat ccaatgatca
    47581 cttattggtc atacaaataa gatgacggca ttttactcgc acgttccatg aaaatccttt
    47641 gcaaagcgta atgtttcaaa tgcacaaaac ttttatactc taagaaatca ggattgcacc
    47701 agtacaagca gcctgatgtt tgacgagtat ttaacttgtt atgaataaca tagaatagca
    47761 gccattcact ttttctacga atcaggaacc ctccatgagt aagtctgaaa atctttacag
    47821 cgcagcgcgc gagctgatcc ctggcggtgt gaactcccct gttcgcgcct ttactggcgt
    47881 gggcggcact ccactgttta tcgaaaaagc ggacggcgct tatctgtacg atgttgatgg
    47941 caaagcctat atcgattatg tcggttcctg ggggccgatg gtgctgggcc ataaccatcc
    48001 ggcgatccgc aatgccgtga ttgaagccgc cgagcgtggt ttaagctttg gtgcaccaac
    48061 cgaaatggaa gtgaaaatgg cgcaactggt gaccgaactg gtcccgacca tggatatggt
    48121 gcgcatggtg aactccggca ctgaagcgac aatgagcgcc atccgcctgg cccgtggttt
    48181 taccggtcgc gacaaaatta ttaaatttga aggttgttac cacggtcacg ctgactgcct
    48241 gctggtgaaa gccggctctg gcgcactcac gttaggccag ccaaactcgc cgggcgttcc
    48301 ggcagatttc gccaaacata ccttaacctg tacttataac gatctggctt ctgtacgcgc
    48361 cgcgtttgag caatacccgc aagagattgc ctgtattatc gtcgagccgg tggcaggcaa
    48421 tatgaactgt gttccaccgc tgccagactt cctgccaggt ctgcgcgcgc tgtgcgacga
    48481 atttggcgcg ttgctgatca tcgatgaagt aatgaccggt ttccgcgtag cgctagctgg
    48541 cgcacaggat tattacggtg tggaaccgga tctcacttgc ctgggcaaaa tcatcggcgg
    48601 tggaatgccg gtaggcgcat tcggtggtcg tcgtgatgta atggatgcgc tggccccgac
    48661 gggtccggtc tatcaggcgg gtacgctttc cggtaacccg attgcgatgg cagccggttt
    48721 cgcctgtctg aatgaagtcg cgcagccggg cgttcacgaa acgctggatg agctgacaac
    48781 acgtctggca gaaggtctgc tggaagcggc agaagaagcc ggaattccgc tggtggtaaa
    48841 ccacgttggc ggcatgttcg gtattttctt taccgacgcc gagtccgtga cgtgctatca
    48901 ggatgtgatg gcctgtgacg tcgagcgttt taagcgtttc ttccatatga tgctggatga
    48961 aggtgtttac ctggcaccgt cagcgtttga agcgggcttt atgtctgtgg cgcacagcat
    49021 ggaagatatc aataacacca tcgatgctgc acgtcgggtg tttgcgaagt tgtgatgatt
    49081 ttgcctcgcc caccgggcga ggcattttat taacggctct gctttctcaa caaatagata
    49141 aaatatggcg cgccgataaa ggtcgacagc agccccgccg ggatctggaa cggaaacagc
    49201 accatccgcc cacaccagtc agcgaacacc agcagtaaac cacccaccag cgccgaaatc
    49261 acgatgtgcg gcatcgtccg tcgaaagccc atcatccgcg cgatatgcgg tgccattaaa
    49321 ccaacaaaac tcaacggtcc aatggtcatc gtcgcggtcg ccgtcaggca agccgctaac
    49381 agcagcagcg caattcgcgt cggcgttaac gcccccccca cggcccgcgc ggtatcacca
    49441 cccagcggta aaatggtcag ccagcggcgg cacagcgggg taatcgccag caaaatcacc
    49501 atcacaattc cggtgcgcca gacctgcgca tcggtggcgt tgtaggtcga accagagatc
    49561 caggtcagca cttgcgccat tcgcgggtca ccactcgcct gcaacatcat taaaagcatg
    49621 gtgaatgcgg tacttaacgc catccccgcc agtaacatgc ggtgtgggga aaatccaccg
    49681 cgcccggcgg cgatcataat aatcaacagc gtcaccgccg caccgatact tcccgcgggt
    49741 aacagccagc caaaagcatt acccggtacc agaaacagca tcagtaccac gccaaacgcc
    49801 gcgccagagc taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga
    49861 ataatacagc ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc
    49921 cagcgccagg gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca
    49981 tcacgaccaa acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg
    50041 gcaaacgcca gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc
    50101 gcgctaatgc tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca
    50161 gtgaccgaac cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg
    50221 gaaagccaga ggatcagcgc accaatcaac gacgccagca tcagtcgcgg cagcagacgc
    50281 cgcgccccca gcatttttgc cagcaacggc gcgaacaacc cgataaagcc gataatcccc
    50341 accgcgttca ccagcagcgc actgatgaca atagccagcg acagcgccgc cagacgcgcc
    50401 agcgacaagg ctagcccgag attgcgcgcc acgccatcat caagccccat cagtgttaac
    50461 ggacgaagta gcagcaacgt cagcatcaca ccgcccagca actgcggcca taaacgctca
    50521 acaccgcccc agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagt
    50581 tggtcatggt ggaagataac cagtaactga ttgattgccc cacaataaag gctcactacc
    50641 agccccgcga ggatcagcgt taccggcgaa agccgtttcc cccacgcgac gccaaagacg
    50701 attaagccaa caacgcaagc gcccgccagc gcagcaaact gactcgccat cgcccctggg
    50761 atcgcccaga gcgtagtgac ggtaatcccc agttgcgcgc ctgtagcaac gccaagtgtc
    50821 gtcggttccg ccagcggatt acgcagcact tgctgaaaca gcacgcccac cagccccagg
    50881 cctgcgccca ccagcagcga aatcgccaga cgcggcagca agctgtagtg aaaaatcatc
    50941 tgctcgatga cgtcaatatc cggcgaccag gcagcctgcg cccactggct acgcggcagc
    51001 gcctgcgaga agttcatcca ggtcagcgcc gtagcggcaa tcaccagcaa cgccagcaat
    51061 aatgccggga aaagcgcaat acgtttactc acgctttacc tccaatggcg ttatccagaa
    51121 cgcgcacaaa gtgcattgcc gagagagttg caccataaaa ccagactgcg ggtacgcgct
    51181 gaaagcgtcc ggcgcggaca aacggcatgg cctgccacag cggcgttgcc attagcgcat
    51241 ccatgtcttt gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca
    51301 gacgatcgat gctgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg
    51361 ggatgccgta ctcatcaaga atttcctgga acaagctgtt tggaccgaag accagcatat
    51421 ggcgcggatc gataagcgtc gtcagcaata acggacgcgc accacgcttc acaaagcggg
    51481 ggttcatgct gcggataaag tcttcatatt gcgctaaatg cgtttctgct gcgctttgca
    51541 ggttaagtaa atccgccatt tccgtcagcg atttacgcgc catcgccagc ggctgtttgc
    51601 cgtcgctgaa gttaaatcca cgccccggcg caatacgagc cagcatttct ggcgaaggac
    51661 catatcccgc cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcgaggttag
    51721 gttctgtacg caaaccgacg tcaatcaccg attcaggtag tggtggttcg ctgacccaca
    51781 gtcgatagtt gatggtgtct gccacgccat aaggcacgat gccgagcgcc agtagtaatt
    51841 ccaccggcag ccactccagc gccacaatac gatttgggtc aatagccgcc gcgtgggcgg
    51901 tattcatctg ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa
    51961 taagaggtaa gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg
    52021 cagaataccc atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc
    52081 aggcgttccc tgagcaatca tttcaccgcc gcgcagggcg accagataat cacagtaacg
    52141 cgcagccata ttgatatcgt gcagcacggc aatgaccgtc aggccacgct cctgacttaa
    52201 acggtgcacc agcgccagca catcaacctg gtgggcgata tccaacgccg aagtcggttc
    52261 gtcgagcaac agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg
    52321 ttcgccgcca gagaggctgt ctaccagccg atgcgccagc ggttttaagc caaccagcga
    52381 gatagcttcc tcaacttttt cgcgatctgc cgcaccaaag cgccctagtg cgccatgcca
    52441 cgggtaacga ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg
    52501 ctgcggcaaa taagccactt tgcgggcgaa cgctttgctg ctccagcttt ccagcggctg
    52561 ggcatcaaga agaatctccc cttccgacgg cggctgatga cggccaagca ttttgagcag
    52621 agtggattta ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt
    52681 taacgacagc ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc
    52741 aaaagtggta tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaacaggca
    52801 cggaaatccg tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga
    52861 cgttctgcgc cccagaagca gccataagtg ttaaagcagc tggcgacgta ttcacgatcg
    52921 aacaggttgt taacatgcag cgccacgttg gagcccgcca tgccgactcg cgccagatca
    52981 taacgcacta acgcatccac gaccgtataa cttcccactt taaaggagtt agccggatca
    53041 ccatagctgg agccagtata acgaccaccg gtgcccagcg tcagacctga aagcggaccg
    53101 tcaaagaagg tatagtcagc ccacagcgaa gccatgtgtt ttggcacctg tgcaggcgta
    53161 ttgcctttat aggtagtatc ggtggtgtat tccgcatcgg tgtaagtata agaaccgact
    53221 acgttaacac tcgccgacag cgccgctttc gcttcgattt ctacgccacg tgcgcggatc
    53281 tcgccacctt caaccgagaa gaaggaaccc tcagggtccg ccatcaggtt gttggtttta
    53341 gtgagattat acacggcacc agtaactaca atcggacgat cttccggtac atatttcacg
    53401 ccgacctcat actgcttacc tttagacggt gcgaaaatat taccatcctt cccaacttgc
    53461 gaagaaggtt caaacgattc gctatagcta aagtaaggtg ttacgccatt atcaaacagg
    53521 tagttaacac caccacgcca ggtaaactgt ttgtcatcac gtttatcggt cgtcccggca
    53581 acgcggttaa gagattcttg atctgcccag tcataacgac cgcccagggt gaccagcact
    53641 ttatcccact gcgcctgatc ctgaacataa acgcccgttt gtttctgctt attcagaatg
    53701 cggtaagggc cggagtttgc cggatctttg gcattgaagt cgaaatcggt attcaccgga
    53761 ttgtacagat cgagcagcgg tacggagtcg tcgtaaccaa accaggcgtt gatgtcatta
    53821 cgcatacgca taaagtcgac accggtcagc agggtgtggt cgatatcgcc agtggcaaac
    53881 ttgctctgca actgggtatc aacggagaag ttttgcagct tctcatcatc aacgacgtat
    53941 ttacgtgcca gataatggcc tttatccgct ggcgctaatg ccgcacactg tttgctgtaa
    54001 gcattcgccg gatcggagca gacgccgtaa ccataaacgc tgttttgcga ggttttgttt
    54061 tcagcaaagc gcaggttctg acgcacagta aaggtgtcgt taaattcgtg atcgaagctg
    54121 tagcccacca tcttctcatt acgagaatag gtgttgttct tcgccccttc gttaaagtct
    54181 gtcggcagac gcttaccgtt cggcagcggc tcaacggttc cctctttcgg caaccagccg
    54241 taataaccgg tttccggctc gttctggaag taagaaagga aggtgaaatt ggttttatca
    54301 tccggacgcc aggtgaacgc cggtgcaata gcataacgct gctcttctga ccctttctgc
    54361 tgggcattgg cagaacgcgc aagaccggtc aggcgataag agtaaacgcc gtcatcatcc
    54421 agcgcatcgc taaagtcaaa accagtctgg aacaggctgt cagtaccggc tttaaactga
    54481 acttctttca gcggttcggt ggtcggacgc ttgctgacca tattcaacag gccgccagga
    54541 ctgcttttac cgtaaagcac ggaaaccggg ccacgcataa tttcagcgcg ttccagcata
    54601 tacggatcaa tgaccgcatc gttatagaag ttgccctgca acttcaggcc attcagatag
    54661 ttattctggc tttggccttc tgccgcaaaa ccgcgaatga tcaggtggtc ataggtgttg
    54721 gatgcgccac gcgtaccaac agagacaccc ggcgtgtagc taagcgcttc ttttaccgac
    54781 ttcggctgat gcagcgccat ctcttcggcg gtcacaacag aaatagactg tggcactttt
    54841 tgaatcggcg tatcggtttt agtgccggta gctgactgtc gcgccgcaat agttgcagca
    54901 ggcccccatg cgctttcttg cggcgcaggt gcagcggtaa cggtgatagt gtcttctttc
    54961 ggttcaaccg ctgcctgtgc ataaacagac atgccgctaa ccgctgtggc tactacaact
    55021 gcgattttac gcagtgagtg ttttggctga gcagttttgg aacgcgccat tggtatatct
    55081 ctgatgtaaa gtgaatgata acgtaaacga gaatgattat tataaagata agataatatt
    55141 cgaaactctg gcgggttcgc aagcggtacg caatctgagc gttcctgaag ggttaataaa
    55201 caaccagatg aaaagaaagg gttaatactt tgggtgggat gttattttac cggatggcaa
    55261 ctcgccatcc ggtatttcac gcttagatgt taattactac caaacatatc cttaatccaa
    55321 ccggctacac cgtcgctgtc tttctgctct tgctgagcag gttgctgttg cggctgttgc
    55381 tgcggctgag aagactgatc aaacggattg cctgacggct gctgctgcat ctcactctgc
    55441 tggcacagcg attgcggatc gctggtccag accggcaaga cacgcatgcc accgctgcaa
    55501 acaaagttgc cgtcgtagtc cacgcccata tctgcaatat cttctggcgg aacaagattc
    55561 agcggcgttg gcgtctggtt agccagataa cgctgataaa tcgacattgc cccgctggct
    55621 ccatacagtt tggtcggctg gttgttatca cggccgaccc aggtgatggt caccgtgctg
    55681 ccgtcaatgc ccgcaaacca ggtatctacg ttattgttgg tagtccctgt tttccctgcc
    55741 agatgcaggt tcgggtattt cgccccaagc tgacgacccg taccgcgttg taccacctgc
    55801 tgcatggtcc atagtgtcag atacgccgcc tgcgccggaa cagcgcgttc cgcctgcggg
    55861 aagctctgat acagcacttt gccatcttcc gcgattaccg aacgcagcgc agaaagcggt
    55921 gcacggttac caccgctggc gatggtctgg aatgcctgcg ccacttcgat tggcgttaag
    55981 ttcaacgccc ccagcagcat tgccggaacc ggatgcaact gatctttcgg tacgcccagt
    56041 ttaatccagg tatccgtaac cgcaggcagc cccagcgcca tccccagatt taccgtcggc
    56101 acgttcatcg aacgggtcaa cgcatccacc agcatcactc tgccgctttc gctataacga
    56161 cggtcatcat tctgcggtga ccagacctgg ccattcggct gacgcagcgc aattggcgca
    56221 tccgcaatcc acgtattcag acgatagatt ttcggctggc ttaaggccgt cagataagtc
    56281 gctggttttg caagggaacc aatcgaacga cgcgcctgca tcgcacggtt gtagcccgca
    56341 aactgcggct cagaacctcc gaccatcgca cgaacttcac cactaaagcg gtcgacgacc
    56401 acaatcgcag tttcaagatc gctcaactta cgctgtttct tcagtgccgg aataccttcc
    56461 acggcggctt tttctgccgc gtcctgggcc accgagtcaa aggtagtgaa gatcttcacg
    56521 ccggagagat cttttacctt atcgcctaat ttagcctgaa gctcctgacg caccagttgc
    56581 ataaaggctg gctgaggaga gatcacccca ccgcgcggct gaacccccag cggacgcgca
    56641 ctcaacatgt catagagttc ttgatcaata atctgttgct gttgcagcag acgcagtacc
    56701 agattacgtc gctccagcgc cagtttcggg ttacgccacg ggttgtagat ggacgctcct
    56761 ttcaccatac cgactaacag cgcctgctga tcgaggctca attcttctac cgggcgacca
    56821 aagtaataca agctcgccag cgggaagccg cggatttcgt tgtcgccgct ctgaccgaga
    56881 tacacctcgt tcatatacag ctcaagaata cggtctttgc tgtaacgcgc gtccatgatc
    56941 agcgccatgt aggcttcatt cgctttacgc caataagagc gctcgctgga gaggaacagg
    57001 tttttcacca gctgttgcgt cagcgtactc gccccctgca ccgtgcgacc tgcggtcagg
    57061 tttgccagca ccgcacgtcc gatggagtag agactgattc catcatgctc gtagaaatgg
    57121 cggtcttctg tcgccagcaa agtatccacc agcaaatccg ggaaaccact gcgcggcaca
    57181 aacaaacgct gctcaccgtt tggtgaggag atcatggtga tcagacgcgg atcaagacgg
    57241 aagaaaccga actgacggtt gttctccata ttgacgatgg tcgccagatg atcgccatca
    57301 aaggtcagac gcgcgcgcac ctgcccttct ttactgtccg ggaaatcaaa cggacggcga
    57361 atcatctcaa tgctgttggc ctgcacggta aattcgccag gacgggtcat tttcgacacc
    57421 tgacgatact gtgttgcctc cagcagcttc accatctcgt tcttgctgat ggtcatgtct
    57481 ggctcaaggt tgaccattcg accataaact gccgcaggca gttgccagac cttgccatca
    57541 atacggctac gaattttttg atcgagataa acgccgtaaa tggcgatcag cacggcaaaa
    57601 acgatagcca gttttaacag cagccatagc cagccgcgtt tgccacgagg cttacgccct
    57661 ttgcctttgc ccttaccttt gcgcggcatc ggttcttcat cctcatagtc atcataatcg
    57721 tcgtaatcat cgtcatcttc gtaacgacga cggcttacct tttgtttgac cggacgcgtc
    57781 ggtttccctt tgcgtccaat tggctcgcgg tcattcccgg ccatgctttt tctccgcaat
    57841 attcaggcgc aaaagcccga ttttctgttc ttctgttaca agacagaaga agatatctct
    57901 caaaatttga gctcgatcgg tcccctctcc cttaagggag agggttaggg tgagggtgaa
    57961 cgtgcgcgct ggtgccctca ccccggccct ctcccacagg gagagggaga aaaccaacct
    58021 tacgaatact ttttcgtccg tcgcgtcggt gccgtgtttg ccgggtcgtc cggccagacg
    58081 tgtttgggat aacgcccttt catctccttt tgcacctcac gatacgcccc tttccagaag
    58141 gcccccaaat cacgcgtgat ttgcagcggc ctttgggcgg gtgaaagcaa ttccagcacc
    58201 agcggcacgc gcccctgggc gatcgtcgga ttagtggcct caccaaacat ctcctgcatt
    58261 ctcaccgcca gcgcgggcgg gttatcttca tgataacgaa tggcgatccg gcttcccgtc
    58321 ggcacagtgt aatgcgcagg caattcacta tccagacgtt gctgcattcc ccaatcaagt
    58381 aatccgcgta gtgcctgata aatgtcgagt gatttcaggc cgcgtagtga atgtacaccg
    58441 gccatatgtg gcagcagcca cgtttccagc gtcgccaaca aactttcatc atcaaccgct
    58501 ggccagtcat attctggcaa ccactttccg gcgcataaca aacgcaagcg tagctgttcc
    58561 gcttccgccg tccagttgag cacgcttaaa cctttatcac ggatgccgtt aagcatcgcc
    58621 tgatgcaact cgtcttctga cggtttcgcc aacggctgca ctttcaccgt caactgaccg
    58681 atttgcagcc gacgccaggc tttcagcgta ccttgcgcgt catcccattc cacggtgtca
    58741 gattgctgca ccagctgcgg acagcgttgt actaattcat caatatcgac cggtaacgcc
    58801 agtaaaatcc gcgcatccgg cgaggcgctg ccctgcaata ataacggcgc gatcaaccat
    58861 tcgtggcgac ttagcgcgtc gtcggcatca agcatcgccc ccatgccgtt tgccagttga
    58921 tagcgtccat cttgcccacg gcgacgagca atgcgatcgg caaacgcccc ggcaagtagc
    58981 ggcgcgataa gcgaactgtc tgcctcaccg ccgcgtacgt ttaagcgttt taacagttgc
    59041 tgactacgtt gctgccaggc tggttgattg cgcgaaaacg ccacgcccag gtcactattg
    59101 cccatccgtg gcggctcttc gagaatggcg gcaatttttg ccgcggtagc agcttcgtcg
    59161 tcgctctttg cgctaaccag cattgccgct aaacgcggat cgttacccag cgctgccatt
    59221 ttttgccctt gcgcactaag ccgttcaccg tccagcgccc ccagcatttg taacagacgt
    59281 ttcgcggcca gtagattcac cgttggcggt tgatccagcc agctcatctg cgccggatcg
    59341 ctgcatcccc attgcagtaa ttccatcagc aaaccggaaa gatcgctttg taagatctcc
    59401 ggttcacttt gcgcggtagc gcgttctgct tgttctttgg cgattaaatg caggcagata
    59461 cccggctcca gacgcccggc gcgcccggca cgctgcgtca tcgacgcctg actgattcgc
    59521 tgagtaatca accgcgtaag ccctgttcgc ggatcaaaac gcgccaccct ttcctgggca
    59581 caatccacca ccagacgaat accttcaatg gttaaactgg tttcagcaat attggtcgcc
    59641 agcaccactt tgcgcatccc ttgcggtgcc gggaggatcg cttttcgctg atcgttcagc
    59701 gacaacgcgc catacagcgg gcagagcaat acatcactgc cgatgcgcga agccagttgt
    59761 tcctgcacac gctgaatttc tccgacgcca ggtaaaaata acagtaatga tccgctttcc
    59821 tgacgcagca tttcagcggt ggctaccgca acggcatcgt caaaacgctg atgcgtgggc
    59881 agcggtaaat aacggcgttc aaccggaaac gagcgccctt ctgagatgac gacgggcgct
    59941 tctggcagca tttgctgcaa gcggtcgttg tccagcgtag ccgacataat cagcagttta
    60001 aggtcatcac gcagaccttg ttgcacatcg agtaacagcg ccaacgccaa atccgcctgc
    60061 aagctacgct catgaaattc atcaaggatc accaatccaa caccgctcag ttccgggtca
    60121 cgctggatca tccgcgtcag cacgccttcg gtaactactt ccaggcgggt attcggcccg
    60181 acgcagtttt gcgcacgcat ccggtagcca acggtatcgc ctggcttttc gttaagcagc
    60241 tccgccagcc gttgcgcgac gttacgcgcc gccagacgcc gtggctccag gaggataatt
    60301 ttcccgttaa tgccgggatg cgccagcagt tgcagcggca gccaggttga tttcccggcc
    60361 ccggtcggcg cacttaataa tacctgcggc gcataatcga gagcggtaag taattcaggt
    60421 aacacggcag caacgggcaa cgacgacaca aaacgctcca gagggttaac attcttcgcg
    60481 ccacattgta gcatcgcggt aattcataac cgagtgcctc acatgtctga cccgcaacgt
    60541 ctgttctttg ctatcgactt acctgcagaa atccgcgaac agattatcca ctggcgcgcc
    60601 acacacttcc cacttgaggc gggacgtccg gtcgccgccg ataatttgca tctgactctg
    60661 gcatttttag gcgaagtgag cgcagagaaa gagaaggcgc tttctctttt agccggacgg
    60721 attcgtcaac ctggtttcac actcacgctt gatgacgccg gccaatggct gcgttcgcgt
    60781 gtggtgtggt tagggatgcg tcagccgcca cgcggcttaa tccagctggc gaatatgctc
    60841 cgttcacagg ctgcccgcag cggttgtttt caaagcaatc gtccgtttca tccacatatt
    60901 accttattgc gcgacgccag cgaggcggtg acaatcccgc cgccaggttt taactggtca
    60961 tatgcggtga cggagttcac cctttacgcc tcctcgtttg cccgtggacg cacacgctac
    61021 acgccgctaa aacgctgggt gctaacgcaa taacaaggat tgtcgcaatg gaattttctc
    61081 cccctctaca gcgcgcgacg ctaattcagc gttacaaacg ttttttagcc gatgtgatca
    61141 cacccgatgg tcgcgaatta acgctacact gcccgaatac gggtgcgatg accggttgtg
    61201 caacgcctgg cgataccgtc tggtattcga cttcagacaa caccaaacgg aaatacccac
    61261 acacctggga attaactcaa agccagagcg gcgcatttat ttgcgtcaac acgctttggg
    61321 ctaacaggtt gacgaaagag gctatcctta atgaatcaat ttcagaactg tcaggctata
    61381 gctcgctgaa aagcgaagta aaatacggcg cagaacgcag ccgtattgac tttatgttgc
    61441 aggcggattc gcgtccagac tgctatattg aagtgaaatc ggttacgtta gcggagaacg
    61501 aacagggata ttttcccgat gcggtcactg aacgaggtca gaaacacctt cgggagttga
    61561 tgagcgtagc ggctgaaggc cagcgtgcgg ttatcttttt cgccgtgctg cattcagcca
    61621 ttacacggtt ttcacccgcg cgccacatcg atgagaaata cgcgcaacta ttgtcagaag
    61681 ctcaacagag gggggtagaa attctggctt acaaagcgga aatttctgct gaaggcatgg
    61741 ctcttaaaaa atcactgccg gttacattgt agtaaagtaa gtaactggtt aatttacatt
    61801 ctggtcgcgt gcgcaaatac gcttttcctc acacagttgt caagtgttac gtttagataa
    61861 ttgctatccg gaaaagcatc tgctatttat agcggcctca tttttccccc gaacatgggg
    61921 atcgatagtg cgtgttaagg agaagcaaca tgcaagaagg gcaaaaccgt aaaacatcgt
    61981 ccctgagtat tctcgccatc gctggggtgg aaccatatca ggagaagccg ggcgaagagt
    62041 atatgaatga agcccagctg gcgcacttcc gtcgtattct ggaagcatgg cgtaatcaac
    62101 tcagggatga agtcgatcgc accgttacac atatgcagga tgaagcagcc aatttcccgg
    62161 acccggtaga ccgtgcagcc caggaagaag agttcagcct cgaactgcgt aaccgcgatc
    62221 gcgagcgtaa gctgatcaaa aagatcgaga agacgctgaa aaaagtggaa gacgaagatt
    62281 tcggctactg tgaatcctgc ggtgttgaaa ttggtattcg ccgtctggaa gcgcgcccga
    62341 cagccgatct gtgcatcgac tgcaaaacgc tggctgaaat tcgcgaaaaa cagatggctg
    62401 gctaattaca gccgttccat cacgtttacc acaggcggga aatgctcccg ccttattttt
    62461 tgttcaaaga gatgacagac acacagtata ttggccgctt cgccccctct ccttccggcg
    62521 agcttcattt tggttctctg attgctgcgc ttggcagcta tttgcaggct cgcgcccggc
    62581 acggtcgctg gctggtacgc attgaagata tcgacccgcc tcgtgaagtt cccggtgccg
    62641 cagaaactat cctgcgccag ctggaacatt acggtctgca ctgggacggc gatgttctct
    62701 ggcaatcgca acgtcaccac gcctatcgtg aagcactcgc ctggttacat gaacaaggac
    62761 taagttatta ctgcacctgc acgcgtgcgc gtattcaaag cattggcggt atttacgacg
    62821 gtcattgccg ggtgttgcat catggaccag acaacgccgc agtgcgtatc cgccagcagc
    62881 atccggtcac gcaatttact gaccagctgc gcggcattat tcacgccgac gaaaaactgg
    62941 cacgggaaga ttttattatt catcgccgtg atggtttgtt tgcctataac ctggcggtgg
    63001 tggtggatga tcacttccag ggcgtgagcg aaattgtccg cggggcagat ttgatcgaac
    63061 cgacagtgcg gcaaatctcg ctgtaccagc tttttggctg gaaagtgcca gattacattc
    63121 atctgccgct ggcgcttaat ccacaaggcg ctaaactttc caagcagaat catgcgcctg
    63181 cgttgccgaa aggcgatcca cgcccggtac taatcgcggc acttcaattt ctggggcagc
    63241 aggcagaagc acactggcag gatttcagcg tcgagcaaat ccttcagtca gccgtcaaaa
    63301 actggcggct taccgcagtg cctgagtcgg caattgtaaa ttcaacattc tcaaatgcgt
    63361 catgctgagc tatgattagc cgctattttt ttgtcctgaa tgatgtttga cactaccgag
    63421 gtgtactatt tttacccgag tcgctaattt ttgccgcaag gtgctaagcc gcgaggaaag
    63481 cgaggctgaa caggcagtcg cccgtccaca ggtgacggtg atcccgcgtg agcagcatgc
    63541 tatttcccgc aaagatatca gtgaaaatgc cctgaaggta atgtacaggc tcaataaagc
    63601 gggatacgaa gcctggctgg ttggcggcgg cgtgcgcgac ctgttactcg gcaaaaagcc
    63661 gaaagatttt gacgtaacca ctaacgccac gcctgagcag gtgcgcaaac tgttccgtaa
    63721 ctgccgcctg gtgggtcgcc gtttccgtct ggctcatgtg atgtttggcc cggagattat
    63781 cgaagttgcg accttccgtg gacaccacga aggtaacgtc agcgaccgca cgacctccca
    63841 acgcgggcaa aacggcatgt tgctgcgcga caacattttc ggctccatcg aagaagacgc
    63901 ccagcgccgc gatttcacta tcaacagcct gtattacagc gtagcggatt ttaccgtccg
    63961 tgattacgtt ggcggtatga aggatctgaa ggacggtgtt atccgtctga ttggtaaccc
    64021 ggaaacgcgc taccgtgaag atccggtacg tatgctgcgc gcagtacgtt ttgccgccaa
    64081 attgggtatg cgcatcagcc cggaaaccgc agaaccgatc cctcgcctcg ctaccctgct
    64141 gaacgatatc ccaccggcac gcctgtttga agaatcgctt aaactgctac aagcgggcta
    64201 cggttacgaa acctataagc tgttgtgtga atatcatctg ttccagccgc tgttcccgac
    64261 cattacccgc tacttcacgg aaaatggcga cagcccgatg gagcggatca ttgaacaggt
    64321 gctgaagaat accgatacgc gtatccataa cgatatgcgc gtgaacccgg cgttcctgtt
    64381 tgccgccatg ttctggtacc cactgctgga gacggcacag aagatcgccc aggaaagcgg
    64441 cctgacctat cacgacgctt tcgcgctggc gatgaacgac gtgctggacg aagcctgccg
    64501 ttcactggca atcccgaaac gtctgacgac gttaacccgc gatatctggc agttgcagtt
    64561 gcgtatgtcc cgacgtcagg gtaaacgcgc atggaaattg ctggagcatc ctaagttccg
    64621 tgcggcttat gacctgttgg ccttgcgagc tgaagttgag cgtaacgctg aactgcagcg
    64681 tctggtgaaa tggtggggtg agttccaggt ttccgcgcca ccagaccaaa aagggatgct
    64741 caacgagctg gatgaagaac cgtcaccgcg tcgtcgtact cgtcgtccac gcaaacgcgc
    64801 accacgtcgt gagggtaccg catgacagtg gcgtatattg ccataggcag caatctggcc
    64861 tctccgctgg agcaggtcaa tgctgccctg aaagcattag gcgatatccc tgaaagccac
    64921 attcttaccg tttcttcgtt ttaccgcacc ccaccgctgg ggccgcaaga tcaacccgat
    64981 tacttaaacg cagccgtggc gctggaaaca acacttgcgc ctgaagaatt acttaatcac
    65041 acacagcaaa tagagttgca gcaaggtcgc gttcgtaaag cagaacgctg gggaccgcgc
    65101 acactcgatc ttgatatcat gttgtttggt aatgaaacca ttaataccga acgactcacc
    65161 gtcccgcatt acgatatgaa gaatcgcggc tttatgctgt ggccgctgtt tgagattgcc
    65221 ccggaactcg tgtttcctga tggggagaca ttacgtgaaa tactcacttc gctagcagta
    65281 ctacaacctg ccatctggta ttagctgtat tattttattt tcttatctta ctcctcactt
    65341 cccctcttac agattaaata tacttaattt acattttagt tttttttaat taattcatga
    65401 aatagcactt catgcaaata gattaggcat tttttgttta aaaaacctag aatagcaaca
    65461 attcaataca ccacgtaaat atattcacac ccgtgataat tcacccctat tacatcctcc
    65521 aaaaactttg agtaatatcc gctcgcaata ttaatttcat ataatttttg taaggatgca
    65581 tgtaatgaaa aaagcacttc tcgcagccgc tctggttatg gcttctggtt ccgccctggc
    65641 tgtagatggt ggtcatatcg actttaacgg tatggtacag tccggtacct gtaaagtggg
    65701 tgtggtagat actggtatgc atagcgttac cactgatggc gtggttaccc tggatactgc
    65761 gaatgttact gatacttttg ctgaagttag cgcaactgct gtcggtttac tgccgaaaga
    65821 gttcatgatt tctgttgagt gtgatccagg tgctccgaag aatgctgagt taactatggg
    65881 ttctgcaagt tacgcgaaca ccagcggtac cctgaataac aatatgaaca tcactgttaa
    65941 cggtattgca ccggctcaga acgtaaacat tgcagttcat aacatgaaaa acaaagctgg
    66001 cgctgctgaa attaagcagg tccatatgaa caactcttct gaagttcagg aactgacatt
    66061 agacgcagaa ggtaaaggcc agtacgtatt taacgcatct tacgttaaag caccgaacag
    66121 cccggctgta actgctggtc atgtaaccac taacgcgctg tacaccgttg cttataagta
    66181 attgatggtg tgttgagcat aaggtttgcc agcaggcaaa ccttattttc gcgaatttaa
    66241 tctttctaca gacagcaagg agtgcttatg tttcccttgg ttaaaaaaac ggttagtgca
    66301 ttatttgtga gtactctgtt agccagtgca ccagcattcg ctgacattat tatttccgga
    66361 acacgtatca tttataacgc tgataaaaaa gatgttaacg ttcgtctgga aaataaaggg
    66421 aatcgcccct tactgattca gaactggttg gatacgggcg atgacaatgc cgatcccagc
    66481 cagatcaaag tgccgtttac cgcgacccca ccagtttcac gcgtggagcc aaaacgcggt
    66541 caaaccgtaa aagtgatgta taccggtgca acagccctgc cgaaagaccg tgaaagcgtc
    66601 tactggttta acgtgctgga agtgccgcct aagccgaaag atgctgaggc tgacaaaaac
    66661 ttgttacagc tggctttccg cacacgcatc aaactctttt atcgcccgag tgggttacag
    66721 ggagaaccgg ctgaagcacc ggcgaaaatc acctggaaat taaacaacgc tcagttacag
    66781 gcaaataacc caacgccata ttacgtgtcc tttaatgagg tcaagctgga aagcggcggc
    66841 aaaacctaca acgtcaattc cagcatggtc acgccgttca gccaggcaag ctttggcgta
    66901 accagtctgc caggaagcgt gtcatccgga aaagtggtct ttaaagcgat caacgacttt
    66961 ggcggcaaca ttgatggttc cgcaacattc taaaaaataa acggattctt tttaacatcg
    67021 ccaggaatgc aaccttgtac cagtttactc atcaaaaaag ccgtatcccg aaaaaaacgc
    67081 tacttgcggc ctgttgtgcc ctgttttata gcagcaacgg tgctgcggcg gacaccgtgg
    67141 aatatgacag ttccttttta atgggaactg gcgcatcaac gattgatgtt aaacgttatg
    67201 ctcaaggcaa cccgacaccg ccgggtctct ataatgtccg cgtatttgta aacggtcagg
    67261 cgacttccag cttagaaatt ccgtttgtgg atattggcga aaacagtgcg gcggcctgtc
    67321 ttacccataa aaacctggcg caacttcaca ttaagcaacc tgaacagcct gtcactttac
    67381 tcgccagaga aggtgaagaa gaggattgtc tggatctggc aaagtcatac gaaaaggcgg
    67441 atgtgtgctt tgacggtagt gaccagtttc tcgatctgac gatccctcag gcctatgttc
    67501 tgaaaagcta tggcggctac gttgaccctt ctttatggga atcgggaatt aacgctgcca
    67561 cactggcata taccctgaac gcgtatcaca caagttcaga taacgacaat agtgacagcg
    67621 tctatggcgc gttcaactca ggtatcaatt taggagcctg gcactttcgt gcgcgcggta
    67681 actataactg gacaacagat aacggcagcg atttcgattt ccaggatcgt tacttacagc
    67741 gtgacattcc ggcaatccgt tcccagataa ttatgggtga tgcctatacc accggtgaaa
    67801 cgtttgactc tgtcaacgtc cgtggtgttc gcctgtacag cgacagccgt atgctgcctt
    67861 cggcgctggc cagttacgct ccgaccatcc gcggtgtagc aaactccaac gccaaagtca
    67921 ccgtgacgca aagcggatat aaaatttatg aaaccaccgt tccgcccggt gaatttgtta
    67981 tagacgacat tagcccttcc ggctttggta gcgaactggt cgtgaccatt gaagaagcgg
    68041 atggttccaa acgcaccttt acgcaaccct tctcgtcggt tgtacaaatg caacgtcctg
    68101 gtgtgggccg ttgggatttc agcgcgggta aagtcattga tgacagtctg cgatccgaac
    68161 ccaatatggg gcaagcctct tattactatg gtctgaataa cctcttcacg ggttataccg
    68221 gcattcagtt caccgataat aactatcttg ccgggctgtt aggtgtgggt atcaacacca
    68281 gcatcggcgc ctttgcggta gacgttaccc attcccgtgc tgaaattccg gatgataaaa
    68341 cctaccaggg gcaaagttat cgcgtgacct ggaacaaact tttccaggat accgggacat
    68401 catttaacct cgcggcgtac cgctattcca cccaggatta cctgggcctg catgatgcgt
    68461 tagtcctcat tgacgacgcc aagcatttgt ctgccgatga agacaaaaac accatgcaga
    68521 cgtactcacg tatgaaaaac cagtttaccg tcagcattaa ccagccattg aatatcgcct
    68581 atgaagatta cggttcgctg tttatttccg gtagctggac gtattactgg gcggcgaaca
    68641 atagccgcac tgaatataat gttggttaca gtaaaagcgt ttcgtggggc agtttcagcg
    68701 tcaacctaca acgtagctgg aatgaagacg gcgagaaaga tgacgcgatg tacgtcagcg
    68761 ttagcgtacc tattgagaat attttaggtg gcaaacgtaa gtcttctggt ttccgcaatt
    68821 taaatactca gctcaatacc gatttcgatg gttcacatca gttgaatgtt aacagttccg
    68881 gtaacactga aaacaatctg gtgaactaca gtgtcaacgc aggttatagc ctcgataaaa
    68941 acgccggcga tttagcctct gttggtggtt atctcaacta tgaatctggg ttaggcggta
    69001 tttccgcttc ggcctcggcc acttctgata acagccaaca gtactccatc tcaaccgatg
    69061 gcggctttgt attacacagt ggtggtttaa cgttcactaa caacagtttc agcagtaacg
    69121 acacgctggt gttaatcaac gccctaggtg ctaaaggcgc acgaatcaat aacagtaata
    69181 acgaaatcga tcgctgggga tatgccgtga cgtcctctgt cagcccatat cgtgaaaacc
    69241 gggtaggtct gaacattgaa acactggaaa acgatgttga actgaaaagt accagcgcca
    69301 ccaccgtacc acgtagcggc tccgttgttt tgacccgttt cgaaactgac gaggggcgtt
    69361 ctgccgtgct gaatattact gccgccaatg gcaaatccat tccgtttgct gcggaggttt
    69421 accagggtga ggtgatgatc ggcagcatgg gccagggtgg tcaggcattt gtacgcggta
    69481 ttaacgacag cggggaatta atcgtgcgct ggtatgaaaa caaccaaacc attgactgta
    69541 agttgcacta ccagttcccg gcgcagccac aaacgcaggg aagcaccaac accttattac
    69601 ttaacaatct tacctgtcag gtagcaaatc actaatatgg aaaattctat gaaaaggata
    69661 ctactaacat ccgcgttaat aggcctgggt ttaccggcgg ttggctctgc aacagatctt
    69721 aatgtcgatt ttacggccac cgtattagcc accacctgca cgataactat agtggaagat
    69781 ggtggtccag cagtgaccgg tagtaacgat gaatacagcc tgacaattcc tgatgttggt
    69841 ctggataaag tcgctaccgc tgcgcccgaa gctcaggcca actttaaatt aaaggcctcc
    69901 gattgcagca atggttactc aaagattttt accacactta caggaacaac ggtttccggt
    69961 aagttaattg ttaatgaagc aacctctggc gctgctgggg tggggatggg gattaagcgt
    70021 agagataccg cagactcgac tttcttcaca cccaataata ctgacaaatt tgaatggagc
    70081 gccgatgaga aagccagcgg tgtgcctctg accgtagcac tgcgcgaaac aacggcggga
    70141 gcaggacgca ccggtgcgtt tcaggcaaaa gccaccttta acttcaccta cgaataacag
    70201 ggacagccac catgaaatta aaagtcatcg ctacactgat tgctactgtt gccgtgggtg
    70261 taagctttaa cagcaatttt gcttctgcga gtacaacgtc cgcttcttta accgtaaaca
    70321 gtaacctgac tatgggtacc tgcagtgctc agataatgga taatagtaat aaagtgatca
    70381 atgaagtggt ctttggcaat gtttatattt ctgaactcgg tgcaaaaagc aaagtgcaac
    70441 agtttaaaat tcgctttagc aattgctctg gccttcccca aaacagcgcc caaatagtgc
    70501 tggcacctaa tggtatatcc tgtgctggtt ctcaatcgtc atcggcgggt ttttctaaca
    70561 agtttactga cgctagcgca gcaaccagaa cggctgtgga agtatggact acagatacac
    70621 cggaaagcaa tggcagtacg caattccatt gtgctcaaaa gataccagtg cctgtgacgc
    70681 ttcccgccga caccacaact cagccttacg attacccgtt aagtgcacgg atgaccgttg
    70741 cggaaggtag attggtaacc gatgtaagac cgggtaattt ccgctctccc acgactttca
    70801 cgatcactta tcagtaatag cggataagga tattagaatg ctatgcaggc atcataaaat
    70861 cgtacacttt ttagggctgg caacagccct tatcacgcct tttgcctatt ccggccagga
    70921 tgtggatctc accgcaaaga tcgtgcccag cacctgtcag gttgaagtca gtaataatgg
    70981 cgttgtcgat ctcggcacag tgacgctgga ctattttgcc gacaatgtaa ccccgacaac
    71041 ggattatgcg ggtggcaaaa cgtttaatgt gaatgtggtc tcttgcgata acattcaaac
    71101 gacacagtcg caaatgaaat tagattttca gccgcaggcg ggatcgcttg cacaggcgaa
    71161 taatcaaatt tttagtaatg aatatgaaca gcaggctacc ggggcgaaaa acgtcggcat
    71221 tgtgattttt tcggcccaac cgaatcagca gacatttaac gtgcgaggaa cggatggttc
    71281 ctcaacagca atttattcgg tagcacctgg caatgctgtt ccctcaacct ggacctttta
    71341 ttcccgtatg cagcgggtga ataatgccct tcctcctgaa tcgggaatgg tgagaagcca
    71401 ggttattgtt aacgtatctt acgaataata aggataatta tgcaaaggaa aggcaataaa
    71461 ctgttgattc agttatgcag tgtgatactg ctatttttta ccacatcctg gtatgcattg
    71521 gcgaatgaat gttatataga gagaaatgct gaaggggatt atcacatgaa gataagctct
    71581 actcagctta gtctggcgtc acaaatggtc gaggttccga cagaaatagc cgaagctaca
    71641 tgggatgtaa atattcaact aagaggcgat gccatagggt gtaaatctct tggggatagt
    71701 aaggcagttc actttcttaa tacagctgac ccaagtttaa tatccacgta caccacaacg
    71761 aatggcgcag cgttattaaa aacaactgtt ccaggcattg tgtattctgt cgagttatta
    71821 tgccttagtt gtggtgccgc agatgaactt gatttatggc tacctgcaca aagtggcgca
    71881 gataacttca taccaagcac ccagacgaaa tgggcctatg agtacagtga tcaaagttgg
    71941 tatttacgtt ttcgcttatt cataactcct gaatttaaac ccaagaatgg tgtttccagc
    72001 ggaacaacga tagcaggaaa gattgcgtca tggtatatag gtaccaatga ccagccgtgg
    72061 atcaactttt acattgacaa tgactcttta aagtttttcg tcgatgaacc gacctgtgca
    72121 acagttgccc tggcacaaga tcagggcaac gtcagtggca atcaggtaac gcttgggaac
    72181 agctatgttt cggaagtgaa aaatgggctt acgcgggaaa tccctttttc tatccgtgct
    72241 gaatactgtt atgccagtaa aattacggtt aagttgaaag cggcaaataa acccagcgat
    72301 gccacactgg tgggtaaaac gactggctcg gcttcaggcg tggctgtaaa agtaaattca
    72361 acttatgaca atagcaaagt attgttaaaa gcagatggta gcaacacggt tgactacaac
    72421 ttcgccgcct ggtcaaacaa cctgctgttt ttacctttta cggcgcagct ggtaccggat
    72481 ggtagcggta atgctgtcgg tgttggaaca ttttcaggta acgcgacctt ctcctttacc
    72541 tacgaataag taaaacgtca ttccctcctc atcgcaccag agttctgctc tggtgcgaac
    72601 atttccccac actaataacc attgcctccc tgttttcggc tgttagaatg cgatcaatct
    72661 gactttagcc atcaggatac gttatgaaac cgaccaccat tgcttcattg cagaaatgta
    72721 agcaggaaaa aaaacgcttc gccaccatca ccgcctacga ctacagcttc gcaaaactgt
    72781 ttgccgagga agggctgaac gtgatgctgg tgggcgactc gctggggatg accgttcagg
    72841 ggcatgaatc tacccttccc gttaccgttg aagatatcgc ctaccacact accgcagtac
    72901 gtcgtggcgc gccaaactgc ctgctgttgg ctgacctgcc gtttatggcg tatgccacgc
    72961 cggaacaagc ctttgaaaac gccgcaacgg ttatgcgtgc cggtgctaac atggtcaaaa
    73021 ttgaaggcgg tgagtggctg gtcgaaaccg taaaaatgct gaccgaacgt gccgttcctg
    73081 tatgtggtca cttaggttta accccacagt cagtgaatat tttcggtggc tacaaagttc
    73141 aggggcgcgg cgatgaagcg agcgatcgac tgcttagcga tgcattagcc ttagaagccg
    73201 ctggtgcaca gttgctggtg ctggaatgcg tgccggttga actggcaaaa cgtattaccg
    73261 aagcgctggc gatcccggtt atcggtattg gcgcaggcaa cgttaccgat gggcagatcc
    73321 tcgtgatgca cgatgcgttc ggcattaccg gcggtcatat ccctaaattt gccaaaaatt
    73381 tcctcgctga aacgggcgac atccgcgctg cggtgcggca gtatatggct gaagtggagt
    73441 ccggcgttta tccgggcgaa gaacacagtt tccattaagg agtgacgttg tgttaattat
    73501 cgaaaccctg ccactgctgc gccagcaaat tcgccgcctg cgtatggaag gcaagcgcgt
    73561 ggcgctggta cctaccatgg gcaacctgca tgatggccac atgaagctgg tcgacgaagc
    73621 caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt aacccgatgc agttcgaccg
    73681 cccggaggac ctggctcggt acccacgcac cttgcaggaa gactgcgaaa agctgaacaa
    73741 gcgcaaagtg gatctggttt tcgcgccctc ggtaaaagag atctacccga acggtactga
    73801 aacccatact tacgttgacg ttccagggct ttcgaccatg ctggaaggcg ccagccggcc
    73861 ggggcatttt cgcggcgtgt cgactatcgt cagcaagctg tttaacctgg tccagccgga
    73921 catcgcctgc ttcggtgaaa aggactttca gcaactggcg ctgatccgca aaatggttgc
    73981 tgatatgggc tttgatattg agattgtcgg tgtgccaatt atgcgcgcca aagacggtct
    74041 ggcactgagt tcccgtaacg gttatctgac ggcagaacaa cgcaaaattg cgcccggtct
    74101 gtacaaagtt ttaagttcga ttgccgacaa attgcaggct ggcgaacggg atctcgatga
    74161 aattattgct attgccgggc aagaactgaa tgaaaaaggc ttccgcgccg atgatattca
    74221 gattcgcgat gccgacacat tgctggaagt ctctgaaacc agcaaacggg cagtaattct
    74281 ggtagccgcc tggcttggcg atgctcgcct gatcgacaac aaaatggtcg agctggcgta
    74341 atacttatac tctaaataat tcaagttgca agttgcagga aggcggcaag cgagtgacaa
    74401 attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga catggatgtc
    74461 tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa cgcagacgca
    74521 gcacatgcaa tttgaagtat gacgagtata actggcgcta cggctgatgg cgccaattat
    74581 taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac gcagacaact
    74641 tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc aacgtcttcc
    74701 cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctctcctc ctgtcttccc
    74761 tgctgaatcc ccttctcaat ccctttctct tcaaaccact gtgccagcgt catcatagac
    74821 ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga
    74881 ccgcgttgca gcatatagtt ttgcatggca actaactgac tttcgctagt gtacccttcg
    74941 tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt
    75001 tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgatg
    75061 gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc cggcgagtaa
    75121 aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg ataaaagaga
    75181 atcggcacta ccagcggtag tttgtcgtga tccgcctcca gatgccgatg catggcggct
    75241 atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg gtgttcaatc
    75301 acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac gtccgtgctg
    75361 tgtcctttaa ggctctcttc aatgaaactc cccgactcta aatgaagcgt gttgaggtca
    75421 caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc cgtctccgca
    75481 tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc atccatcttc
    75541 cctgtcttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc tatcgtcagc
    75601 cacctcttta ttgtttaccg agcagcgttc agagaaatat caattcgtta caggcgatac
    75661 atggcacgct tcggcgcggt acgcaaactg ctgatgcaaa tgccgtcaga tgagtagaca
    75721 ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac cgcctgcttc
    75781 gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc
    75841 accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgaccagg
    75901 attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg aatgtcacca
    75961 acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga attatttctg
    76021 ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc gccagcttcg
    76081 ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat tttgaaggcg
    76141 acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga ttgttatccc
    76201 tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct taagatataa
    76261 agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat ggttgtcacc
    76321 gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa gccgccaaac
    76381 ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcgacg tgaacgtgca
    76441 aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg ataaccatct
    76501 acccgatgca aaaaatgggt atgtgactgg aaatcaaata catcgcgaat ttcgttaagc
    76561 tcagaaacgc tcataaattg cagcgatttt ggttcccact tctgcgggtg acgtttgatg
    76621 cgcgaggtaa caataaacgc cgtcgccttc atgccatatt gtttcaacac aggatacgca
    76681 tagcggctca ctgacttgag gccatcatca aaggtaatca ccaccgctcg cgcaggaaga
    76741 ttgatcttat tcttcacgta accttccagc tgcaccatgc tcagtgtcgc gtatcccctg
    76801 tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg
    76861 cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aggttagcac cggaaggcca
    76921 ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat acggatctga
    76981 taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc
    77041 gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg cgtatctttc
    77101 caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc gtcttcaact
    77161 ttttgtcgcc cctgaaccgg ctcaagatga cctttatcga taaacccttt atcaaagcca
    77221 aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg tccggcccga
    77281 atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc attttcgatg
    77341 gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag catcagcagg
    77401 agaataacag cttgtttgta catgaaaaaa tcaccgaggg tgaaacaacg cttttattgt
    77461 agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa
    77521 ggcggatttt tttgttgctg gtgccaaata ctactcacct ccggcgcacc cagttcgaca
    77581 atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat ctgttcaatt
    77641 aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa
    77701 gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc ctcaccagag
    77761 tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagaact taatccgcgc
    77821 cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag cacatccagt
    77881 atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt
    77941 agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct
    78001 ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga
    78061 tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag
    78121 tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg atcatataaa
    78181 cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc agtgagtaaa
    78241 agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat
    78301 cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac accgccgtga
    78361 gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg
    78421 aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg ccgacatatc
    78481 cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga
    78541 acttggcacc aaaaaatgat gacgcgacgt tggcgtaggc gttggtgatc accgacatca
    78601 tgatcagccc cggtacgatg aactgcatgt agctgaagcc atgcatatcg ccaatacgcg
    78661 aaccaatcag gttaccgaaa ataataaagt aaagggtcat ggtgatgact ggcggcacca
    78721 gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg
    78781 ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca gtgaaacaaa
    78841 cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact
    78901 taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt ccagcgtcgc
    78961 ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag
    79021 atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg aggtattttc
    79081 caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg cttcttccag
    79141 gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca
    79201 cattgagcgg cgaagttcaa tatccacgcc tgcagtcggc tcgtcgagaa tcagtagttt
    79261 aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat
    79321 gcgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt tttcgctgcg
    79381 gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg
    79441 cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact gacgtttagc
    79501 gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt
    79561 taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg gcccgagaag
    79621 cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct gaacgccgcc
    79681 tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc
    79741 ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa
    79801 acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc
    79861 aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg
    79921 gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca
    79981 gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaacctg
    80041 gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa
    80101 gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc cgcagttgaa
    80161 aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa
    80221 catagctcat tgctcgacga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg
    80281 aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa
    80341 cgcgggcaga aagttaccat tcacggctgg gcttatggca ttcacgacgg cttgctgcgt
    80401 gatctggatg ttactgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt
    80461 tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca
    80521 tccggcaact tcacacttac tcgtccagca gaatcacttt gccgatatac ggcagatgac
    80581 ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac
    80641 cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa
    80701 tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc
    80761 ccgagtcgat gatatcttca acaatcagca catccttgcc acggatatct tcatccagat
    80821 ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca
    80881 taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg
    80941 agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa
    81001 tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta
    81061 cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt
    81121 tttggcaccg caacaaagcg caagacacgc agtataccag caaaacgatt cattcgcgga
    81181 atctgttaat aaagctcaaa ttgtgatgac gatcacacat gttaaaacct ataattaatt
    81241 gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct
    81301 cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac
    81361 tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc
    81421 ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgtgccgcg ctttggctat
    81481 acgccgccct gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct
    81541 gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt
    81601 ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtggcac
    81661 tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag gaaatcaacg
    81721 gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc gatcaggact
    81781 ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa caaattaacg
    81841 ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc
    81901 cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc gacacccttt
    81961 acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc aaagagaaat
    82021 ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc tgccgtggcg
    82081 tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc
    82141 gtcgtatcat tcttccggtc aatgatggtc gcctgattgc gattaacgct gaaaacggca
    82201 agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca
    82261 ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa accatcgtga
    82321 tggcaggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt
    82381 ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg
    82441 caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcgtgg gcaccagcgg
    82501 cctatgacgc gaagctggat ctggtgtatc tgccgatggg cgtgaccacg ccggatatct
    82561 ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg
    82621 ccacaaccgg gaaattggcg tggagctacc agaccgttca ccacgacctg tgggatatgg
    82681 atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccagtta
    82741 tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat ggcgaactgg
    82801 tggttccggc accggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc
    82861 ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata
    82921 tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct
    82981 atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg ggtaacctgg
    83041 gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc
    83101 caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac ccgatggagc
    83161 agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac
    83221 cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag
    83281 catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta
    83341 ttggtacgcc gcaggacagt atgccgttcc cgatgcctgt accggtgccg ttcaatatgg
    83401 gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta
    83461 cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc
    83521 gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggt aagcagtatg
    83581 tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggtgac tatattgtgg
    83641 cttatgcgct gccggatgat gtgaagtaag acttgctcag attgctgaca acgtgcgcgt
    83701 tgttcatgcc ggatgcggcg tgaacgcctt atccagccta caaaattgtg caaaatcaat
    83761 ggattgcaca ggtaacgtag gcctgataag cgtagcgcat caggcagatt tgcgtttgtc
    83821 atcagtttaa tgcccggaga gatccgggca tattcccgaa tacggtcttt ttataccgta
    83881 aaccctaaca tcatccccgt atcttcatgc tccagcagat ggcagtgcgc catataagca
    83941 cgttctttcg gtgcgtcgtg attaaacttc accagcactt cactgacatt gccttctacc
    84001 ttaacggtat ctttccagcc cgcgcgatga gccgctggcg gtttgccatt ttctgacaag
    84061 atacggaact gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc cacgccagag
    84121 ataacccaac gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac
    84181 gcctgaccgt tgattttatt ggcatggtgg aaatcgaact tcccgccgtg gttcatatga
    84241 ttcatattgc cgtgccccat atggcccatc atctggctgt gatccattcc ggccatcgcc
    84301 tgatcgccat atttctccat tagcatctgc atccccatca tatcgagcat cgggtccata
    84361 gaaagttgca gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg caggctactt
    84421 aatgtgtctg gcaaagcacc ggaagcacta atagcaatcg gctgaatccg cattaccgga
    84481 tgaggcttat caaacggcgc aatcgccatc cccatctggc tgaccggcag cgtcactagg
    84541 tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcacc
    84601 ggcagctcgt tcaccttcac cggttcaggt agcagaccac cgtcgctggc aatcacataa
    84661 agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg cattacagcc attgagcaaa
    84721 cgcaggcgca gccaaccacg cggggcagcg tgttgcgggt agattgcacc gttggtcagc
    84781 aacgtatcgc caaaccagcc cacggcggcg gtcatcacat ccagttgata atcaatctgc
    84841 ccgtcggcgc taaatttctt atcctgaacg atcaccggaa catcatcgat accccactgt
    84901 tttggcagca ttaatttcag gatctcgtca tcttcaatca ccaccagacc agccagcccc
    84961 atcgccacct gtcgcccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca
    85021 ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgtggg
    85081 ccgccgtcga cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc
    85141 agttggttat agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc
    85201 agcagattgc cgttatagcc ccaggtagtt gcagttttct cgccaaaggt agactgacct
    85261 gcgccaatag ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggatcggt
    85321 aacgttgggc gttccgccgc aaagactgca cggctccaca gtggcaaggc tgaagccaca
    85381 cccagtgcga cggaatattt caagaaatca cgacgttgca tagttatttc cttattctta
    85441 agcaggcgaa acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc
    85501 gcagacacaa tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta
    85561 tcttctttaa gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca
    85621 gcctgatggc cgtttgcgca aacagttacg cgctcagtga gtctgaagcc gaagatatgg
    85681 ccgatttaac ggcagttttt gtctttctga agaacgattg tggttaccag aacttaccta
    85741 acgggcaaat tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg gatctcagta
    85801 attacgacac cttcgacatg aaagctctcg gtgaagacag ctaccgcgat ctcagcggca
    85861 ttggcattcc cgtcgctaaa aagtgcaagg ccctggctcg cgattcctta agcctgcttg
    85921 cctacgtcaa ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca
    85981 atgttagcta ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca
    86041 tggccgaaaa aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg
    86101 tagataacgt tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg
    86161 ctgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt
    86221 ttatctatca tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg
    86281 tgctgattat cggcggcggc gacggtgcca tgctgcgtga agtaacccga cataaaaacg
    86341 ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt gtcgttctgc cgtcagtatc
    86401 tacccaacca taacgccggt agctacgacg atccgcgctt taagctggtg atcgacgatg
    86461 gcgtcaattt cgttaatcaa accagccaga cctttgatgt cattatctcc gactgcaccg
    86521 atcctatcgg tcccggcgaa agccttttca cttcggcatt ttatgaaggc tgcaaacgtt
    86581 gcctgaatcc tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag
    86641 aagccatcga cagccatcgc aaactcagcc attacttcag cgacgttggc ttttatcagg
    86701 cggcgatccc gacctattac ggcggtatca tgacttttgc atgggcgaca gataacgacg
    86761 ccttacgcca tctctcaacc gaaattattc aggcgcgttt tctcgcctct ggcctgaaat
    86821 gccgttatta caatccggca gtccatacgg cagcttttgc cttaccccag tatctgcaag
    86881 acgcactggc ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc
    86941 atggctttaa taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca
    87001 aaactgccga agagcgcgac ggttatattg cttatatcga tgaactctat aatgccaacc
    87061 gtctgaccga aatcctgtca gaaacctgtt ccattatcgg ggctaatatt cttaacatcg
    87121 cccgccagga ttacgaacca cagggtgcca gcgtcactat tctggtgagt gaagaaccgg
    87181 ttgacccgaa actcatcgac aaaacagaac accccggccc actgccagaa acggtcgttg
    87241 cccatctcga taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg
    87301 gtttatgtac cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga
    87361 aggcgctgaa ttacctgatc caccagcttg agtccgatat cgtaaccatt gattatcgcg
    87421 tgcgcggttt tacccgcgac attaacggta tgaagcactt tatcgaccat gagattaatt
    87481 cgattcagaa ctttatgtct gacgatatga aggcgctgta tgacatggtg gatgtaaacg
    87541 tctatcagga aaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact
    87601 acatgttcca caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg
    87661 cgctgtggaa agaaatgcgc gagatttatt acgggcgcaa tatgccagct gtttaacggc
    87721 tctggcggag ctcccaggct ccgccagatt tatttacttc tgctgcacga aattgcggta
    87781 agccgccacg acctgcagaa aatcctcaac gccgcatagc gacaggcttt cttcgtcgta
    87841 gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat
    87901 cacctcttcg ccgtccatcc atagcgtata ctcatgccct gcccgctgcc aggaacgttc
    87961 gctacctttc agtgcgtgcg ctgcttgttc cacttcatca agcaaggcca ggttttcttt
    88021 cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatac gcacctttac
    88081 cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa
    88141 tgcaccgaaa tcgcattgcg ccattgatgt aattatcgca gccttaccgg agaaaaacag
    88201 cggaacggcg atggtttaga aataaaaaaa tgcccgggaa tctcattcac cgggcattgt
    88261 gtcgtttatg cgcagcgcgt gcgctgactt tttaaaccgc agtctggaaa atcaccccat
    88321 cggctttctc ggtgtactga gaaagctggt tgaagttcag ataacggtaa gtatcaacgg
    88381 ctgttttatc tacctgcgcc acgtaggtct ggtactcttc cggcgtcggc agtttgccaa
    88441 tcagcgccgc aacagcagcc agttccgcag aagccaggaa gacattcgcg ccagtaccca
    88501 gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt tgcaccgtcc gccacacgcg
    88561 cctggttacc catacacagg gaacagccag ggatctcgat acgcgcacca ctcttaccga
    88621 agacgctgta gtagccttct tcggtcaact gtgcggcgtc catacgggtt ggcggtgcca
    88681 cccacaggcg ggtcggcaac tgacctttgt gcgcatccag cagtttacct gcggcacgga
    88741 agtgaccgat gttagtcatg caagaaccga taaacacttc gtcgatcttc tcgccctgta
    88801 ccgcagacag cggacgtgcg tcgtccgggt cgttcggagc acagaggatt ggctctttaa
    88861 tatccgccag atcgatgtcg atcactgccg cgtattccgc atctgcatcg gcttccagca
    88921 gctcaggatt cgccagccat ttttccatgc cctgaatacg acgttccagg gtacgacgat
    88981 cgccgtaacc ttccgcgatc atccacttca gcaggacgat gttggagttc aggtattcga
    89041 tgattggttc tttgttcagc ttgatggtac aaccggcagc agaacgctcg gcggacgcat
    89101 cggtcagctc aaaggcctgc tcaactttca gatccggcag accttcaatt tctaggatgc
    89161 ggccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct tgtttgatcg
    89221 catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc attttgcctt
    89281 tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt gcggctgcaa
    89341 atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg gtatgggagt
    89401 caccaccggt accgacggta tccggcagca gcatacggtt cagccaggag tggattacgc
    89461 cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc agcgtgtggt
    89521 gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac tgcatcacca
    89581 ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc atcgggcctg
    89641 tggtgtcctg agagcctaca gaagtcattt tcggttcaca gtacgcgccc ggacgaatgc
    89701 ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg cgatcgctct
    89761 cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt gcttcacgcg
    89821 ctttggtggt caggccacga ccgataatca gcggaatacg gccgccagca cgcacttcat
    89881 caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgccggtt tcgtggttac
    89941 gtacttcacc tttgtacggg taaacgtcaa ttacgtcgcc catgttcagg ttagagacat
    90001 ccacttcgat tggcagcgca cccgcatctt ccatcgtgtt aaagaagatg ggtgcaattt
    90061 taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata tcatcgccca
    90121 taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc acaacgtcac
    90181 cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg atcgggccaa
    90241 caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt
    90301 gcaatgggat atccgggcgt gaccacgcat ccggtgccgg agaaaggtca tcggtgttgg
    90361 tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc gggcgattca
    90421 ggaaccattc ggcatcagcc caggactgca taacctgctt cgcatattcg ttgcctgctt
    90481 tcgctttctc ttctacgtca tagaagttat cgaacatcag tagtgtgtga gaaagtgctt
    90541 tggcagcgat cggtgccagt ttggcatcat ccagcgcatc gatcagcgga tgaatgttgt
    90601 aaccaccctg catagtaccc agcagttcga tggctttttc cggagtcagc agtggggatt
    90661 tggcttcgcc tttggcgaca gcagccagga agcctgcttt aacataggcg gcttcatcga
    90721 cgccaggagg aacacggttg gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg
    90781 ggtttttcag cagctctaca agcgcggcca tttggtttgc atccaggggt ttgggcgcaa
    90841 tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg acggttctcc
    90901 tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga gacagcagtt
    90961 tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac gacgtgttaa
    91021 tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt
    91081 gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc tcaacgaatc
    91141 ccaagattag tcaaaattta aactaccgcc tctttatact cggattcaca gcacctgcgg
    91201 gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa tgactttgcc
    91261 gtttaaaccc catgtgctgg cgctaatttg cagtgccggg ctttgtgccg cctctgccgg
    91321 gctatatata aaaagccgca cagtggaagc gcctgtggaa acgcaatcga cacaactggc
    91381 tgtgtctgac gctgccgcag ttacgcttcc tgcaacggtt tccgcacctc ccgtaacacc
    91441 cgccgtcgtc aaatccgcat tcagcactgc acaaatagat caatgggtcg cgcccgtcgc
    91501 gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc cgacaaacgt
    91561 tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg ctgctattca
    91621 ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct ttccacaatt
    91681 gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc
    91741 ccagccgcag gacgtgatgg actcggtaca acgattgcgg caactggcac aacaaaccgg
    91801 ctcgctgaag tcatcaaccg aacagaaagt tattaccaca acgaagaaaa ctgtaccggt
    91861 aacacagaca gtcacggctc ccgtcatacc atccaatacc gtttcaactg ccaaccctgt
    91921 cattacagag cctgcaacaa ccgtcatttc cattgagccc ggcaatcctg atgtggtcta
    91981 tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg cgtatccgcc
    92041 ggtttatctg ccaccaccag ccggagaacc gtttgttgac agctttgtac gcggattcgg
    92101 ctatagcatg ggcgttgcta ccacgtacgc actattcagc agcatcgact gggatgacga
    92161 cgatcatgac catcatcatc atgacgatga taattatcat caccacgatg gcggtcatcg
    92221 tgacggtaat ggctggcaac ataacggcga caacatcaat atcgacgtca acaatttcaa
    92281 ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta
    92341 ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc aaactgatgt
    92401 caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc agcgtcaggc
    92461 ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac gagataccca
    92521 acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct atgacgactt
    92581 ccgcgacttc agccgtcgcc aaccactgac ccagcaacaa aaggacgccg ctcgtcagcg
    92641 ttatcagtca gcttctcctg agcagcgcca ggcagttcac gagaaaatgc agactaaccc
    92701 gcagaaccag cagcgaagag aggcagcgcg tgagcgcatt cagcccgcct cgcctgagca
    92761 gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc gaagagacgc
    92821 agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta aggaaaaagt
    92881 acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg ttcaatcagc
    92941 atcacctgaa caacgtcagg tttttcggga gaaagttcag gagagccgcc cacaacgtct
    93001 aaacgacagt aaccatactg ccaggctgaa taacgagcaa cggtcagcag tacgcgaacg
    93061 tctctctgag cgcggagcaa ggcgactgga aaggtaaatt acaggcgtaa aaaaagcggc
    93121 gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta cttcttcttc
    93181 gctttcgggt tcggcaggtc agtaatgcta ccttcgaaca cttctgccgc caggcccaca
    93241 gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc
    93301 atttcaattg ccaggccgat ttcacccagc agctcgccgc cgttagtacc gacaatcgca
    93361 ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag
    93421 tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc atagctgatg
    93481 cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg ttcggtatag
    93541 gcgatggacg ggataacttt cggatcgaag tagtgtttct taccggcgat aacttcagcg
    93601 gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccgac gatatcgccg
    93661 atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat gaaaccacgg
    93721 tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg cacacgacca
    93781 atcgctacca gcacggcgtc gtaacgctgc ggttcagctg gtgctttttt gccttccatc
    93841 gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc cagcatcagg
    93901 ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc agctgccggg
    93961 ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg
    94021 cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg tacttctttc
    94081 agttccagcg cgtcagtaga gtcccagata cgcggatctt catgcggaat aaacggcagt
    94141 tgaatcgggc gagaacccgc tgcaatgatc gcgttgtcga agttgatcac ggttttaccg
    94201 ttctcacctt caacttccag ggtgttagcc ccggtaaatt tacccagacc gttgaccact
    94261 ttgactttgc ggcctttcgc cataccagcc agaccaccag tcagctgatt gatcactttc
    94321 tctttccagg tacgaatctt gtcgatatcg gttttcggtt cgccgaagac gataccgtgt
    94381 tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc tttagaaggg
    94441 atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac gattacggtt
    94501 tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc tgccccaagt
    94561 accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt atctccggcg
    94621 ggtctgacgt tttttttatg acgcccatta tccaccgggt cgttctatcc gtcggtattt
    94681 tacaaaattg ttaacaattt ttaaacaaca aacggcaacc gatttgtcta ttcgctaata
    94741 acctcaatac attcatgaga ttaccagaaa aaagccggcc gttgggccgg ctcttttact
    94801 tacatcacca gacggcgaat gtcagacagc gtgttgttaa tgatggtaat gaaacgggca
    94861 ccatcagcac cgtcgatcac gcggtggtcg aaggagagag aaatcggcag catcagacgc
    94921 ggcacgaact ctttaccatt ccacaccggc tccatcgcgg acttggaaac gccgaggata
    94981 gccacttccg gcgcgttcac aatcggcgcg aagtgggtag tacccaggcc gccgatgctg
    95041 gagatggtga agcaaccgcc ctgcatttcg cccgcagtca gcttaccgtc acgcgctttc
    95101 ttagaaatag tcatcagctc gcgagacagt tcgatgatgc ctttcttgtt gacgtctttg
    95161 aataccggaa caaccagacc gttcggggta tccaccgcca caccgatgtt gatgtatttc
    95221 ttcagggtca gacgctgacc gtcttccgac agcgaactgt tgaagcgagg catctgctca
    95281 agcgctgcag caacggcttt catgatgaag acaaccgggg tgatcttcac atccagctta
    95341 cgtttcgccg cttcttcgtt ctgctgttta cggaacgctt ccagctcggt gatatcggtt
    95401 ttgtcgaagt gagtaacatg cgggatcatc acccagttac ggctcaggtt cgcaccagaa
    95461 attttctgga tacggcccag ttccacttct tcgatttcac caaacttgct gaagtccacc
    95521 ttcggccacg gcagcatgcc agggatacca ccgccagtcg ctgccggagc tgcttctgca
    95581 cgtttgatag cttctttcac gtaagcctga acgtcttcgc gcaggatacg acctttacgg
    95641 ccagtgccct tcactttcgc aaggttaacg ccaaactcgc gtgccagacg gcggatcagc
    95701 ggggtcgcgt gaacgtaagc gtcattttca gcaaattcag atttgccttc cgctttcgca
    95761 gccggtgctg ctgccggggc ttcagctttt gctgccggag ccggcgctgc tgcttcctgt
    95821 ttcgcaggag ctgccgcagg cgctgcgcct tcaacttcga agatcataat cagcgagcca
    95881 gttttcactt tatcgccaac gttgactttc agttccttca cgacgcctgc aaacggagcc
    95941 ggaacttcca tagaagcttt atcgccttct acggtaatca gtgactgttc agcggcaact
    96001 ttgtcgccca ctttcaccat cacttcggtc acttcaactt cgtcaccgcc gatatccgga
    96061 acgttaactt ctttcacgcc agccgctggt gcaggggccg ctgccggagc cgcttcctgt
    96121 ttagccgccg gagctgccgc gcctgcttca cccgccactt cgaagaccat aatcagcgag
    96181 ccggtagaca ctttgtcgcc ggtgttcact ttgatttctt tcacggtgcc tgcaaacgga
    96241 gccggaactt ccatagaagc tttatcgcct tctacggtga tcagcgactg ctcagcttca
    96301 actttgtcgc caactttcac caggatttcg gtcacttcaa cttcgtcgct gccgatatcc
    96361 ggaacgttaa cgtcttttgc cgcggcagcc gcggggactg ctgccggagc tgcttctttc
    96421 ttctcttctg cctgagcagg tgcagcgtct gctgcaccgt cggcggaatc gaaaatcata
    96481 atcagtgcgc cggtctgagt tttatcgcca acagagactt tgatctcttt aacgataccc
    96541 gcctgcggag acggaacttc catagaagct ttgtcgcctt ctacggtgat cagcgactgt
    96601 tcggcttcaa ctttgtcgcc cactttgacc aggatctcgg tgatttcaac ttcatcagcc
    96661 ccgatgtccg gtactttgat ttcgatagcc attattcttt tacctcttac gccagacgcg
    96721 ggttaacttt atctgcatcg atgttgaatt tggcgattgc gtcagcaacc actttcttat
    96781 cgatttcgcc acgtttagcc agttcgccca gcgccgcaac cacgacataa gaagcatcaa
    96841 cttcgaagtg gtgacgcagg ttctcacggc tgtcggaacg accgaagcca tcagtaccca
    96901 gtacgcggta gtcgtcagcc ggtacgtaag tacggacctg ctcagcgaac agtttcatat
    96961 agtcggtaga tgccacagcc ggagcgtcgt tcatcacctg agcgatatac ggaacgcgcg
    97021 gagtttccag cggatgcagc atgttccagc gttcacaatc ctgaccatca cgcgccagtt
    97081 cggtgaagga ggtcacgcta taaacgtcag aacctacgcc gtaatctttc gccaggatct
    97141 ctgccgcttc acggacgtga cgcaggatag aaccggagcc cagcagctga actttacctt
    97201 tgctaccttc aatggtttcg agtttgtaga tacctttacg gataccttcc tcagcacctt
    97261 ccggcattgc cggcatgtgg tagttttcgt tcagcgtggt gatgtagtag taaacgttct
    97321 cttgtttttc accgtacata cgctccagac cgtcatgcat aatgacagca acttcgtaag
    97381 cgtaagccgg gtcgtaagag atacagttcg gaatagtcag cgactgaatg tggctgtgac
    97441 catcttcgtg ctgcagacct tcaccgttca gggtggtacg accggaagta ccgccgatca
    97501 ggaagccacg cgcttgctgg tcgccagccg cccagcacag atcgccgata cgctggaagc
    97561 cgaacatcga gtaatagatg tagaacggaa tcatcggcag attgttggtg ctgtaagagg
    97621 tcgccgctgc cagccaggaa caacctgcgc ccagctcgtt gatcccttcc tgcagaatct
    97681 gacctttctc gtcttcttta tagtaagcaa cctgctcgcg gtcctgcggg gtgtactgct
    97741 gaccgttcgg gctgtaaata ccaatctgac ggaacagacc ttccataccg aaagtacgcg
    97801 cttcgtcggc gatgatcggt accagacgat ctttgatcga cttgttcttc agcatcacgt
    97861 tcagggcacg aacgaaagcg atagtggtag agatctcttt gctctgctct tccagcagcg
    97921 cgccgaagtc ttgcaggctt ggcagctcaa gcttctcggt gaagttcggc tgacggcttg
    97981 gcagataacc gtgcagtttc tgacgctgtg cgtgcagata ggtatgctct tcagaacctt
    98041 ccgggaaggt gatgtacggc agtttttcga tatctgcatc agacaccggc acattgaaac
    98101 ggtcgcggat gtgacgcaca ccgtccatgt tcattttctt aacctggtgc gcgatgtttt
    98161 taccttcagc cgcgtcgccc atgccgtaac ctttaatggt atgagcaagg attaccgtcg
    98221 ctttgccttt ggtttcctgc gctttcttga atgcagcgta gattttcttc ggatcgtgac
    98281 caccacggtt cagtgcccag atctgctcgt cagtccagtc tgcaaccagt gctgcggttt
    98341 caggatattt accgaagaag tgttcacgaa cgtacgcacc atctttcgat ttgaaggtct
    98401 ggtagtcgcc gtcaacggtt tcgttcatca gctggatcag tttaccgctg gtatctttac
    98461 gcagcagttc atcccaacgg ctaccccaca tcactttgat cacgttccag ccagcacctt
    98521 cgaagatacc ttccagttcg ttgatgatct tgccgttacc ggtgaccggg ccgtcaagac
    98581 gctgcaggtt acagttgata acgaagacca ggttatccag tttttcacgg gtagcgatgg
    98641 tgatcgcacc tttggattcc ggttcgtcca tttcaccgtc gccgaggaac gcgtaaacgg
    98701 tctgtttaga ggtatctttc aggccacggt gttccagata tttcaggaat ttagcctggt
    98761 aaatagcacc aatcggaccc agacccatag atacggtcgg gaactgccag aattccggca
    98821 tcaatttcgg gtgcggatag gaagagaggc cattgccgtg aacttcctga cggaagttat
    98881 ccagctgctc ttgagtcaga cgaccttcca ggaaagcacg agcgtacacg cccggggaga
    98941 tgtggccctg gaagtaaacc aggtcgccgc catcctgctc gttgcgtgca cggaagaagt
    99001 ggttaaagca cacatcataa atggttgcgg aagactggaa ggacgccatg tggccgccca
    99061 gttcgaggtc ttttttcgac gcacgcagaa ccgtcatgat ggcgttccag cggatagctg
    99121 aacgaatacg gcgttccagt tccagattac ccggatactc cggttgttct tcaacgggga
    99181 tggtgttgat gtagttgctg ataccggtgc ctgcggctac gttgacaccg cctttgcggg
    99241 cttcagcaag cagttggtcg atcagatact gagcacgctc aacaccttct tcacggatga
    99301 ccgattcgat cgcctggagc cagtcgcgag tttcgatcgg atccacgtca tttgggaaac
    99361 gttctgacat gggttattcc ttatctatct aataacgttg agttttctgg aacctgtccc
    99421 attgaactct cgccggaaag ctcaataaga caggttctac gtttagttgc cgcgctttta
    99481 tatgcgcttg atttacaaca tcttctggat aatttttacc agaaaaatca ctaattcttt
    99541 cgttgctcca gacgacgcag agaacgctca cggcggcttt cttcacggct tctgtcgagc
    99601 aaaatttctt cgataaaggc cagatggcga tgcgatgctt cgcgcgcttc ttccggctta
    99661 ccggccataa tcgcttcaaa tatgcgggtg cggtgactac tcaccagcgg cagcatctcg
    99721 cgacgcgaat agagcaattc gaagttctgg cggacattct gggccaacat cggctccata
    99781 caccttagca gatgaagcag aaccacattg tgggccgctt cggtgacggc aatctgatac
    99841 tggagtacgg cgtttgattc cgcgtccaga tcgccagact gctgcgccag ttctatggcg
    99901 tggtggagtt cacggatgcg ttccttgtct tcatcggtac tacgcagcgc ggcgtaataa
    99961 gcggcgatac cttccagggc gtgtcgtgtt tcgagcaagt catactgtga ttcaggatgg
   100021 tcggagagca gctccaccag cggatcgctg aagctttgcc ataggctgct ctggacaaaa
   100081 gtgccgccac cctggcgacg aagcaacaag cccttcgctt cgagacgttg aatcgcctca
   100141 cgcaatgagg gacgggagac gtcaaactgt tttgccagtt cgcgttccgg tgggagtttt
   100201 tcgcccgggc ggagagtgcc ttcgaggatc aaaaactcca gttgctgctc aatcacatcg
   100261 gagagttttg gttggcggat tttgctgtag gccatgagtt cctgtcttaa gccacttgcc
   100321 gaagtcaatt ggtcttacca atttcatgtc tgtgacgcta aagtaacaaa gtattcacct
   100381 tatgtccata caggttttga ttgaaatcat gaaactgtgc acattttaac aacttgacat
   100441 atataacgtt tcaaagttgt a
//
LOCUS       DAZKFZ010000002        69601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000002 DAZKFZ010000000
VERSION     DAZKFZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(27..2339)
                     /gene="pbpC"
                     /locus_tag="ACHOQW_000090"
     CDS             complement(27..2339)
                     /gene="pbpC"
                     /locus_tag="ACHOQW_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP9571245.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(2340..7301)
                     /gene="yfhM"
                     /locus_tag="ACHOQW_000091"
     CDS             complement(2340..7301)
                     /gene="yfhM"
                     /locus_tag="ACHOQW_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP9571246.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7508..8353
                     /gene="sseA"
                     /locus_tag="ACHOQW_000092"
     CDS             7508..8353
                     /gene="sseA"
                     /locus_tag="ACHOQW_000092"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP9571247.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8696..8821
                     /gene="timP"
                     /locus_tag="ACHOQW_000093"
     CDS             8696..8821
                     /gene="timP"
                     /locus_tag="ACHOQW_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP9571248.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8853..9629)
                     /gene="sseB"
                     /locus_tag="ACHOQW_000094"
     CDS             complement(8853..9629)
                     /gene="sseB"
                     /locus_tag="ACHOQW_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP9571249.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9772..11055)
                     /gene="pepB"
                     /locus_tag="ACHOQW_000095"
     CDS             complement(9772..11055)
                     /gene="pepB"
                     /locus_tag="ACHOQW_000095"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP9571250.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11233..11433)
                     /gene="iscX"
                     /locus_tag="ACHOQW_000096"
     CDS             complement(11233..11433)
                     /gene="iscX"
                     /locus_tag="ACHOQW_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP9571251.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11445..11780)
                     /gene="fdx"
                     /locus_tag="ACHOQW_000097"
     CDS             complement(11445..11780)
                     /gene="fdx"
                     /locus_tag="ACHOQW_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP9571252.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11782..13632)
                     /gene="hscA"
                     /locus_tag="ACHOQW_000098"
     CDS             complement(11782..13632)
                     /gene="hscA"
                     /locus_tag="ACHOQW_000098"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP9571253.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13649..14164)
                     /gene="hscB"
                     /locus_tag="ACHOQW_000099"
     CDS             complement(13649..14164)
                     /gene="hscB"
                     /locus_tag="ACHOQW_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP9571254.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14260..14583)
                     /gene="iscA"
                     /locus_tag="ACHOQW_000100"
     CDS             complement(14260..14583)
                     /gene="iscA"
                     /locus_tag="ACHOQW_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP9571255.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14600..14986)
                     /gene="iscU"
                     /locus_tag="ACHOQW_000101"
     CDS             complement(14600..14986)
                     /gene="iscU"
                     /locus_tag="ACHOQW_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP9571256.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(15014..16228)
                     /gene="iscS"
                     /locus_tag="ACHOQW_000102"
     CDS             complement(15014..16228)
                     /gene="iscS"
                     /locus_tag="ACHOQW_000102"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9571257.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16340..16828)
                     /gene="iscR"
                     /locus_tag="ACHOQW_000103"
     CDS             complement(16340..16828)
                     /gene="iscR"
                     /locus_tag="ACHOQW_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP9571258.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17129..17866)
                     /gene="trmJ"
                     /locus_tag="ACHOQW_000104"
     CDS             complement(17129..17866)
                     /gene="trmJ"
                     /locus_tag="ACHOQW_000104"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP9571259.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17985..18788
                     /gene="suhB"
                     /locus_tag="ACHOQW_000105"
     CDS             17985..18788
                     /gene="suhB"
                     /locus_tag="ACHOQW_000105"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP9571260.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18933..19787
                     /gene="yfhR"
                     /locus_tag="ACHOQW_000106"
     CDS             18933..19787
                     /gene="yfhR"
                     /locus_tag="ACHOQW_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9571261.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            19978..21258
                     /gene="csiE"
                     /locus_tag="ACHOQW_000107"
     CDS             19978..21258
                     /gene="csiE"
                     /locus_tag="ACHOQW_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP9571262.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21250..22389)
                     /gene="hcaT"
                     /locus_tag="ACHOQW_000108"
     CDS             complement(21250..22389)
                     /gene="hcaT"
                     /locus_tag="ACHOQW_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP9571263.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22549..23439)
                     /gene="hcaR"
                     /locus_tag="ACHOQW_000109"
     CDS             complement(22549..23439)
                     /gene="hcaR"
                     /locus_tag="ACHOQW_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP9571264.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23575..24936
                     /gene="hcaE"
                     /locus_tag="ACHOQW_000110"
     CDS             23575..24936
                     /gene="hcaE"
                     /locus_tag="ACHOQW_000110"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP9571265.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24933..25451
                     /gene="hcaF"
                     /locus_tag="ACHOQW_000111"
     CDS             24933..25451
                     /gene="hcaF"
                     /locus_tag="ACHOQW_000111"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP9571266.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25451..25771
                     /gene="hcaC"
                     /locus_tag="ACHOQW_000112"
     CDS             25451..25771
                     /gene="hcaC"
                     /locus_tag="ACHOQW_000112"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP9571267.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25768..26580
                     /gene="hcaB"
                     /locus_tag="ACHOQW_000113"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25768..26580
                     /gene="hcaB"
                     /locus_tag="ACHOQW_000113"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP9571268.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26590..27792
                     /gene="hcaD"
                     /locus_tag="ACHOQW_000114"
     CDS             26590..27792
                     /gene="hcaD"
                     /locus_tag="ACHOQW_000114"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP9571269.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27889..28311
                     /gene="yphA"
                     /locus_tag="ACHOQW_000115"
     CDS             27889..28311
                     /gene="yphA"
                     /locus_tag="ACHOQW_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9571270.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28359..29231)
                     /gene="yphB"
                     /locus_tag="ACHOQW_000116"
     CDS             complement(28359..29231)
                     /gene="yphB"
                     /locus_tag="ACHOQW_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9571271.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29243..30337)
                     /gene="yphC"
                     /locus_tag="ACHOQW_000117"
                     /pseudo
     CDS             complement(29243..30337)
                     /gene="yphC"
                     /locus_tag="ACHOQW_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30370..31368)
                     /gene="yphD"
                     /locus_tag="ACHOQW_000118"
     CDS             complement(30370..31368)
                     /gene="yphD"
                     /locus_tag="ACHOQW_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9571272.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31393..32904)
                     /gene="yphE"
                     /locus_tag="ACHOQW_000119"
     CDS             complement(31393..32904)
                     /gene="yphE"
                     /locus_tag="ACHOQW_000119"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9571273.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32927..33910)
                     /gene="yphF"
                     /locus_tag="ACHOQW_000120"
     CDS             complement(32927..33910)
                     /gene="yphF"
                     /locus_tag="ACHOQW_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9571274.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(34007..37288)
                     /gene="yphG"
                     /locus_tag="ACHOQW_000121"
     CDS             complement(34007..37288)
                     /gene="yphG"
                     /locus_tag="ACHOQW_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP9571275.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37406..38599
                     /gene="yphH"
                     /locus_tag="ACHOQW_000122"
     CDS             37406..38599
                     /gene="yphH"
                     /locus_tag="ACHOQW_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9571276.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38662..39915)
                     /gene="glyA"
                     /locus_tag="ACHOQW_000123"
     CDS             complement(38662..39915)
                     /gene="glyA"
                     /locus_tag="ACHOQW_000123"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9571277.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40243..41433
                     /gene="hmpA"
                     /locus_tag="ACHOQW_000124"
     CDS             40243..41433
                     /gene="hmpA"
                     /locus_tag="ACHOQW_000124"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP9571278.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41478..41816)
                     /gene="glnB"
                     /locus_tag="ACHOQW_000125"
     CDS             complement(41478..41816)
                     /gene="glnB"
                     /locus_tag="ACHOQW_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP9571279.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41877..43211)
                     /gene="glrR"
                     /locus_tag="ACHOQW_000126"
     CDS             complement(41877..43211)
                     /gene="glrR"
                     /locus_tag="ACHOQW_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP9571280.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43201..43914)
                     /gene="qseG"
                     /locus_tag="ACHOQW_000127"
     CDS             complement(43201..43914)
                     /gene="qseG"
                     /locus_tag="ACHOQW_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP9571281.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44079..45506)
                     /gene="qseE"
                     /locus_tag="ACHOQW_000128"
     CDS             complement(44079..45506)
                     /gene="qseE"
                     /locus_tag="ACHOQW_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP9571282.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46082..49969)
                     /gene="purL"
                     /locus_tag="ACHOQW_000129"
                     /gene_synonym="purI"
     CDS             complement(46082..49969)
                     /gene="purL"
                     /locus_tag="ACHOQW_000129"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP9571283.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LISGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50226..51782
                     /gene="mltF"
                     /locus_tag="ACHOQW_000130"
     CDS             50226..51782
                     /gene="mltF"
                     /locus_tag="ACHOQW_000130"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP9571284.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51779..52282)
                     /gene="tadA"
                     /locus_tag="ACHOQW_000131"
     CDS             complement(51779..52282)
                     /gene="tadA"
                     /locus_tag="ACHOQW_000131"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9571285.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52340..52975)
                     /gene="yfhb"
                     /locus_tag="ACHOQW_000132"
     CDS             complement(52340..52975)
                     /gene="yfhb"
                     /locus_tag="ACHOQW_000132"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP9571286.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53184..54032
                     /gene="yfhH"
                     /locus_tag="ACHOQW_000133"
     CDS             53184..54032
                     /gene="yfhH"
                     /locus_tag="ACHOQW_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9571287.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54088..54348
                     /gene="yfhL"
                     /locus_tag="ACHOQW_000134"
     CDS             54088..54348
                     /gene="yfhL"
                     /locus_tag="ACHOQW_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP9571288.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(54542..54622)
                     /gene="shoB"
                     /locus_tag="ACHOQW_000135"
     CDS             complement(54542..54622)
                     /gene="shoB"
                     /locus_tag="ACHOQW_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP9571289.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(55044..55424)
                     /gene="acpS"
                     /locus_tag="ACHOQW_000136"
     CDS             complement(55044..55424)
                     /gene="acpS"
                     /locus_tag="ACHOQW_000136"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9571290.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(55424..56155)
                     /gene="pdxJ"
                     /locus_tag="ACHOQW_000137"
     CDS             complement(55424..56155)
                     /gene="pdxJ"
                     /locus_tag="ACHOQW_000137"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP9571291.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(56167..56895)
                     /gene="recO"
                     /locus_tag="ACHOQW_000138"
     CDS             complement(56167..56895)
                     /gene="recO"
                     /locus_tag="ACHOQW_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9571292.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(56907..57812)
                     /gene="era"
                     /locus_tag="ACHOQW_000139"
     CDS             complement(56907..57812)
                     /gene="era"
                     /locus_tag="ACHOQW_000139"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9571293.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(57809..58489)
                     /gene="rnc"
                     /locus_tag="ACHOQW_000140"
     CDS             complement(57809..58489)
                     /gene="rnc"
                     /locus_tag="ACHOQW_000140"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9571294.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(58762..59736)
                     /gene="lepB"
                     /locus_tag="ACHOQW_000141"
     CDS             complement(58762..59736)
                     /gene="lepB"
                     /locus_tag="ACHOQW_000141"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9571295.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(59752..61551)
                     /gene="lepA"
                     /locus_tag="ACHOQW_000142"
     CDS             complement(59752..61551)
                     /gene="lepA"
                     /locus_tag="ACHOQW_000142"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9571296.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(61749..62228)
                     /gene="rseC"
                     /locus_tag="ACHOQW_000143"
     CDS             complement(61749..62228)
                     /gene="rseC"
                     /locus_tag="ACHOQW_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP9571297.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(62225..63181)
                     /gene="rseB"
                     /locus_tag="ACHOQW_000144"
     CDS             complement(62225..63181)
                     /gene="rseB"
                     /locus_tag="ACHOQW_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP9571298.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(63181..63819)
                     /gene="rseA"
                     /locus_tag="ACHOQW_000145"
     CDS             complement(63181..63819)
                     /gene="rseA"
                     /locus_tag="ACHOQW_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP9571299.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(63852..64427)
                     /gene="rpoE"
                     /locus_tag="ACHOQW_000146"
     CDS             complement(63852..64427)
                     /gene="rpoE"
                     /locus_tag="ACHOQW_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP9571300.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(64424..64579)
                     /gene="rseD"
                     /locus_tag="ACHOQW_000147"
     CDS             complement(64424..64579)
                     /gene="rseD"
                     /locus_tag="ACHOQW_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP9571301.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            64835..66457
                     /gene="nadB"
                     /locus_tag="ACHOQW_000148"
     CDS             64835..66457
                     /gene="nadB"
                     /locus_tag="ACHOQW_000148"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP9571302.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(66442..67179)
                     /gene="trmN"
                     /locus_tag="ACHOQW_000149"
     CDS             complement(66442..67179)
                     /gene="trmN"
                     /locus_tag="ACHOQW_000149"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP9571303.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            67311..68645
                     /gene="srmB"
                     /locus_tag="ACHOQW_000150"
     CDS             67311..68645
                     /gene="srmB"
                     /locus_tag="ACHOQW_000150"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP9571304.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(68678..69559)
                     /gene="yfiE"
                     /locus_tag="ACHOQW_000151"
     CDS             complement(68678..69559)
                     /gene="yfiE"
                     /locus_tag="ACHOQW_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9571305.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
BASE COUNT        16522 a        19119 c        17505 g        16455 t
ORIGIN      
        1 ttagattaat aaatattttg tatatttcac tgaaatgaaa aatgtactga cgcgatttgc
       61 cctacgtcat ccatcaccag caattgataa tcacctttat ccgtcaaatg cagggtcacg
      121 ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc accagcgttc acctgcccct
      181 ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg gtaaacgttt aataatctcg
      241 ccatcgcgaa cgcctgtcag ctgcagcggg agctgggcat cgtgaccgta tggcgggcaa
      301 attgtcgagg ccggtggtaa gcgcacagcg cgacgttctg atgcgggcaa ccagggttcc
      361 agcggtagcg gccagacatt tatcatttct tgtcgcgctt gcgggcaatc agcggcaaca
      421 cgtttgccat tttcatccag ccagatgggg aaacgaatgc cattaatgcc ttcctgctcc
      481 ggcagtaata gagttggcgg ctgactcccg tccagcagcc aggttgccag gcggcggcga
      541 cagttaccgt caccttccgg caaagactgt ccgcccggcc agcaaataac gccacgagtg
      601 actgagttcg ggcgcgggtc ttccggcaga ttagcactgc gcgacagtaa gatattattg
      661 acctgattca acaatggtac ggcgctggca aaaccaaact gaccaacaac gggcgtgccg
      721 tccggtctgc cagtccagat cccaatgaca tagcgagcgt taaccccaat cgcccaggcg
      781 tcacgatagc catagctggt gcccgttttc catgccagtg gggcgacgcg cggcaaggca
      841 ccatccggca agggtagcgc ttcatcagcc ataatccggc gaatgatcca cgccgctccc
      901 gacgacatta aaggccgttc aagcagcgga tcgtcaggct gtaagcgcaa tttgcctgcc
      961 ttgccgtggc gagcaaacgc ggtatacgct gccgccatat cttccagttt cgcaccagcg
     1021 ccgccgagaa tgagcgaaag attcggcgca gcaccgttgg gcaaatataa cggcaatcca
     1081 acattgcgta actttgccgc aaaccgtttc ggtccatagg cttccagcac ctgcacagca
     1141 ggtaaattca gcgagcgcac cagcgcctcg ctcatgctga tcgggccatg aaaaccgctg
     1201 tcaaagttgc caggtcggta atcaccggta cgccggggga cgtcttgcag cagtgatgcc
     1261 gggtggatca atccttcatc cagcgccaga ccgtaaacaa acggtttgag caccgatcct
     1321 ggcgatcgga tcgcattgac catatcaaca tgaccaaagc gtgaatcatc gttgagatca
     1381 accgaaccca cccagccgcg aacacgcata tcggtatgat caaccacgat catcgccagt
     1441 gagctgcgcg gtggtaatcg tcctttccag ttttgcgcca gttcttccag acgtcgttga
     1501 agaccggcat ccagcgtagt aacgattttg tcgcttttgc ttttaccgag catcatgcgc
     1561 gaaaacagcg gtgccagttg cggcatttgt cggggtgcca gccagatggg ttcttccctt
     1621 gactctttta cctgctcacg ggaccacaca ccttgcacgg ccatccgttc gagcacttta
     1681 ttacgcgcga cttcggcacg ctccggccaa cgatccgggc gaagacggct gggcgcttgc
     1741 ggcaaaaccg ccagcattgc cgcctcggaa tagcttaaat tcgcaggcga ctttccgaga
     1801 taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc gcggttaaga
     1861 tacaaggtca gaatttcacg cttagacaga tgccattcca gttgcaacgc gcgccagagc
     1921 tggcgaattt tgccgccaaa tgttttgggg tgaggatcaa gcagacgagc aacctgcata
     1981 gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca tgctgcgcgc
     2041 gccaccgaga atggattcac cccaggatgc ttccagaacc agcgatcttc ataattgatc
     2101 agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta caggataacg ccagatgccg
     2161 tcagcatcgg cgaagcgcca gagcggcgta ccatcctgcg ccacgaccac tcgtgcggga
     2221 ttgacttcat gcagcggtag aggccagagt ttatctgctc cccacgctgc aagaaaaagg
     2281 agaaatggcg cagctgccag cgttatccag cagccgcgtt tggttaacaa gcgaggcatt
     2341 tacggtctga caatcagcaa atcttcagcc gcgccggtcg cccgccattg gggaacatac
     2401 attgattcca ccatcggttg cggtacctga tacgttcccg gcgtcaccgc ccgcgccaga
     2461 tacaccagcg ttaccggttg gtattcatca acggcaaccg ccgccacaaa gcgatcgtca
     2521 cggaactcaa tgtgcttaat actcgcctgc tgcatctggt tcagtaagtt ctgcacttcg
     2581 ccaccacttt gctccaggct ggcgctaccg ttcgccagat tctggttttc cagttccaga
     2641 cccgcaggca gcagatccac gactaacgca tccggcacgc tgttactagc ttttacctgc
     2701 aaccagacca gcaccagatc gccgctacgt aacgagtcca gcgatttgct cttaccatca
     2761 gtaccaagaa tatgacgctc aatttgcagc acattgttcg ccggtaaagg tgcggattgc
     2821 ggataaccgc tggcatccac acgcagccat aacggctgct cgccactgtt ggtcacctgc
     2881 aaggtggcaa gttgatcgct attcagattg ctgttttgcg ctttctcgcc tgtcagcggc
     2941 tcagctgaga aagaggtttg cgcctgccat ttacctggta aatcctgaat cgtacgggca
     3001 gccaggaaca acgcgttact ttcctgcgtc gatagccagc gttcaccaaa cgcctgctgc
     3061 gaaagtgtgt tcagcaggga gtactgctca tcgggtagca gtttgttctc ttccagcaag
     3121 gagagcatca acgcgttgtc gcgcagtgga ctaccgtaat cacccagcca tatccgctca
     3181 tcactattac gcggcgtttt tagcgccagc gcaatcgctt cttcatcacg cgtcgcatca
     3241 cccatggttt tcagcgcaac gccaagttgc agcagcggta aaccagaagc ggcatctgca
     3301 cgatgctccc agatttcacg cagcgcaccc agcggagcct tttgctgacg ggccaacacc
     3361 agcgcagcgt aagactgtac ggcgaattta ctggctttga gattatccgc gtacgggatc
     3421 gacatcatgc ccggatcttg taaatagcgc agcagacgct cattaccccg gttaatggcg
     3481 tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca ggaaatccat cacgtaagcc
     3541 gtcagccagt actcttcgtc accgttttta tcccacagcg caaagccgcc gttatcacgt
     3601 tgcatttgca gcaaacggga aatgccgata tcgaccgatg cacggcgttt ctcatcactg
     3661 tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg tataaagtga cgggaacagg
     3721 ccgctggcgg tttgctcaag acagccgtac ggatacgctt ttaactcttt gatataacgt
     3781 gcgatgttca gcggtggttt gccgctcaac aacaattgcc cttccagcgt aacaggcgag
     3841 aagttttgca atccatccgc cggaatcgcc catgtctcac caggctgtaa cgccgtaccg
     3901 taattaaccg tttgtgccgg gaacgccgga cggacgccga ttttccactg cttatgctga
     3961 tcggcaacgg tttcacccgg taacgctaac ccgctaatgg tggcctgaat ttctccatcg
     4021 ccataacccg gcaatgctcg caccgggata aacagcgtag tacgcacacc tggcgctaat
     4081 tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac cactggcggt cagggcaacg
     4141 ttcagttttt gcggtttatc ggtaagatta gtgatatcca gcgtcagacg cgagttatcg
     4201 ccactcgcca taaagcgcgg catgttcagt tcagcaatca ccggtgcggc aactatcact
     4261 ttgctttcgt tgctaccgaa ttcatctgcc gtccaggctt gcgccatgac gcgcaattca
     4321 ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc cctgttcgtt gagcgttacc
     4381 ggcagcgcct gctgcgcgac aatattgacg tgattgaccg gcggtttacc accacgtttc
     4441 agctcatcac catcgccacc gaaacgcaga gctgccagac gcccctgacc ttcaataacc
     4501 tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct gaccaaagaa cgcctgccac
     4561 ggatctggcg tgacgtagtc agtaatattc agcacaccac tatcaacggc agacaccagc
     4621 acattcacct gtttaggctt ctcgccattt ttagtgctgg ctttaatttt cacggttaat
     4681 ggttgattgg gacgcatttt tgctggtgtt tccagcgcca gatcgaggcg acggttttca
     4741 tcaccaagcg gcagatgcaa cacaccaacc gcgcgttttg gcgtcgcgga gcgagattta
     4801 tcgccaggac gcaccaccag cgtactcaaa taaagatcat gacgattcca ggttttatcg
     4861 accggaatcg tcagatccag cccttgagcc ggaacatcaa tctcttgcca ccacagcggc
     4921 ccttcactgg actcgaccat cgcataacct ttacccgccg ttggcgcggc gatatgcaac
     4981 ttaatggtat cgccagggcg ataaccggct ttatccagtt tcagcgtgac acggtcgggt
     5041 cgcactgcgc cgctaccgtc gctattatcc tgccagctat agccagccca aaaacgaaca
     5101 ctactgaccg cttcattcgg cgctttgact tccagacgat aagcgcccca ctctaccggg
     5161 aaactgacct tgccggtttc gtccgctttc agatccagag tttgttcatt ttcaatcaga
     5221 tctttttgat caaactgtga ttgccagcct tcatcttctg accagttcca gtagtaatcg
     5281 cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg cttttttcac gccttgcgca
     5341 tcgctataaa cgatgtcaaa agcggcgtta ctgccttcat caacaatggg ctgtttcacc
     5401 gtgctgtcag tgcgataatc gtaaaccgat ttcgaggcga actgcggacg gatccccggc
     5461 aatgcatcgg caggccagat agcctgctca gcgcggcgcg tcaccgggcg accgcccgat
     5521 tccagcaaac tgccctggaa aataacctgc aatggggaat gcgtttcctt ccactggctt
     5581 tctgtagaaa cttcgccgcg ccctttatca tccagcgtca actgaacttc atccagcgtg
     5641 cgggaaaggt tttcggcagc tatatcgccg aattcaaaac caggtaaggc tgacacagcc
     5701 tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat taccattagc aggcgcaccg
     5761 tacaggtagt atcccaccac ggagaatttc acttcatcat tcggcgtaag cggggttttc
     5821 tcaccggtca gattcagcgc catacgctct ggcataaaat cttcgacgtg gaaatcccac
     5881 atccgatact ggttatcgcc cgtgttagcg cgaatatgcc acataccggt tgccgcattg
     5941 ctatcgagtg gccaggtaaa gtggtagagg ccattttccg gctgactaac gacgctcctg
     6001 agtacctgcc catcgggttt aatcacgtct aacttgatgg gttgattggg caacgcttta
     6061 ccgtctgcat cacgcagcaa accattgagg attaccgttt cacccgggcg ataaagatcg
     6121 cgcgggccaa acatgaaaaa ctgtttgcta tagcctggcg cgccagcaat gttaaattct
     6181 gctaagtcca gcgccggaag ttttaaatcg agtagcgttg tctgaccgtc tttacgtgcc
     6241 agcaataatg cggcgttttt atcattttcc agctgcacat gcccctgagc gtcactggtt
     6301 gcctgagtca gagtctgccc tttctcattt aataaagaga cttcaattcc ttgctgggcc
     6361 gcgccgtttt ccagactttg ggtaaagata tccagacgat tgtgataacg gtgagctgaa
     6421 acgccgatat cacttaacgt aaacagcgtc gcgggattac tgtaatcgta acgtccagcc
     6481 tgattcatca cagccagata cacgcccgcc tgctgaagcg gtttgatatc gcccagcggc
     6541 agcaataatt tttcacgggt gttacgcgca ggattgagat caaaccgtcc ggtgtagacc
     6601 agatccgcca tctgcagcag tttgtctgac tgccagttcg ccagcgaatt gcggtattcc
     6661 cattggctaa tgaatgctgg cagagattct ggcttaacgc ggaagaagtt aacatcgaca
     6721 ttattaacgt tgagcgccat taccggcagc ccttcaacga ctttaccagg cagcagcgaa
     6781 ccacggctgg caaaaccgac actgggttgg atgtcgcggg tagttatagt tttttcgtaa
     6841 tctttactga aggttgcgtt gttgagtgct ttgacctcct tgccaattgt aacgatcaaa
     6901 tcacgtttcg gttcgaggtg gcgtaaacgc agctctttaa gattatctga tagctcccag
     6961 gcaccatcca ctttgccgct ttttttatcg acgacatgaa taacgcgtga gaaatcctga
     7021 tccgggtcga gagggatgga gaacgtcagc accagcgtgg ctgcaccatc aagttggact
     7081 tcagagagat ccagcaatgt cagcgcctta ccggcactct gttgggccag tttttgtcgc
     7141 tctggcgcgg agagttttgc tgaactcgcg ttttctgaag aggccgcttt agtaacttca
     7201 gaaggcgcat cttttttcac cgctgttggc gcgttatcgt tgttgtcgca ccctgccagc
     7261 gccagcatta gcatgcaggc ggctacgcgt aactttttca tttttcatcc ctgccacaat
     7321 ggcccgttag caacgtcgaa taattattat gcgtgagaat ttacgttatg taattcagta
     7381 tcacgcccat acaatccgaa ttttagtgag aattgtggct cagacagcca aatcagcctc
     7441 ttttgcttgt cgcttttcac taaacatccg acaatttagc ctaaccccgg caaaaatgga
     7501 gatgcctatg tccacgacat ggtttgtagg agccgactgg ctcgccgaac atattgatga
     7561 cccggaaatt cagattatcg atgcccgcat ggcgtcgcct ggacaggaag atcgtaacgt
     7621 tgctcaggag tatctgaatg gacatattcc cggcgcagtg ttttttgata tcgaagcgct
     7681 ttctgatcac acttccccgc ttccgcacat gctgccgcgc ccggaaacgt tcgccgtggc
     7741 gatgcgtgaa ttaggcgtta accaggataa gcacctgatt gtctatgacg aaggtaatct
     7801 tttctcagcc ccacgagcat ggtggatgct gcgcaccttt ggtgtagaga aagtgtcgat
     7861 tctggggggt ggacttgcag gctggcagcg cgatgatctg ttgttagaag aaggtgcagt
     7921 agagctgccg gaaggagagt ttaacgccgc gtttaatcct gaagcagtgg tgaaagtaac
     7981 cgatgtgtta ttggcaagcc atgaaaatac ggcgcaaatt attgatgccc gcccggctgc
     8041 acgttttaac gcagaagttg atgaacctcg cccaggttta cgtcgcggac atattcccgg
     8101 tgcattgaac gttccgtgga cggaactggt gcgcgaaggc gaactaaaaa cgaccgatga
     8161 actggatgcg atattttttg gtcgcggcgt cagctatgac aaaccaatta tcgtcagctg
     8221 cggctctggt gtaacagcag ccgtggtttt gttggcactc gcgacgctgg atgtgccaaa
     8281 cgtgaaactg tacgacggcg catggagtga atggggcgcg cgggcagatt taccggttga
     8341 gccattgaaa taagcttttt acaggcaata aaaaaccgcc gaatttggcg gttttttatt
     8401 gctggtctgg ttcgcggcct ttccagcagg ttgacttgtg ttgccggagc aacgcaggca
     8461 ccccacagtg ttgaaatact ctcttttatg cctggctgtg tcaagataat aaacagagta
     8521 attattcaaa catgagagta ttattcttaa tggccctttt cgccgtctcg caaacgggcg
     8581 ctggctttag gaaagaatgt tccatgaccg taaatacagg caccccacag tgcttgctat
     8641 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     8701 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttaaccg aggtaaataa
     8761 taaccggagt ctctccggcg acaatttact ggtgattaac aaccttcaga gcagcaggta
     8821 agcccgaatg ccgccctttg gggcggcata ttttagatta tccgattctg tttaaagtca
     8881 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     8941 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     9001 gtatcggtcg ctacacttcc cgccacctga ataatttctt cgatatcacc atcggcttca
     9061 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     9121 cgcttcacag gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     9181 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     9241 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     9301 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     9361 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagtgc ttctaacgag
     9421 gtaaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgcg
     9481 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     9541 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     9601 tcttcaagtt cattttttgt ttcggacata agaaccacaa ttcattcaac gttcggggca
     9661 caagtgcctg atgcgccata cttatcaggc ctacaaggga ttcacaattt gttgaatttg
     9721 caatactttg taggccggat aaggcgttta cgccgcatcc ggcatattag tttacgccgt
     9781 taacagatta gcaatcgtgc gcacaccaag tcccgtagca cccgcagacc actgttcaac
     9841 cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc cagccttgct gatagttctc
     9901 aacaaagtgc gacaggaagc ccgctgccgt gctcgcgcct gccggatacg ccgcgcttcc
     9961 ggtattgttc agttcggcaa agttagacgg cagctggctg cggtggaatt ccgccagcgg
    10021 cagacgccag aatggttcgt tctcttgtga ggcactcgcc agcaaacgac cggcaagcgc
    10081 atcgtcaaaa ctgaacagcg cgtgataatc attacccagc gcagttttcg ccgccccggt
    10141 gagggtcgcc gcatcaatga tcatttccgg tttctgcgca ctggcatcaa tcagaccatc
    10201 ggcaagcacc aggcgccctt cggcatcagt gttcatcact tcaacttttt taccgttgcg
    10261 ataagtgatg atatcgccca gcttgaacgc attgccgcta atcaggttat ccgcacagca
    10321 gaggaacagc ttcacgcgct tgttcagtcc gcgcgtaatg gcaaatgcca gcgccccggt
    10381 aaccgttgcc gcgccgccca tgtccgactt catcgagtcc ataaacgcag tctgtttgat
    10441 gctgtagccg ccggagtcaa aagtgatacc tttacctacc aggcacgcgt acactggcgc
    10501 ttctttatcg ccagttgggt tgtaatccag cgccagcaat accggagaac gttctgaacc
    10561 gcgtccgacg gtgtgcagcc ccatataacc ttgctcacgc agatcttcgc ctttggtgat
    10621 ccgataagtc acacgatcgc tcgcgacgtt gctgatcaga tcaacagcac gctgtgccag
    10681 ttgcgatggt cccaattctt ctgccggtgc gttgatggtg tcacgcaccc agtcgatgat
    10741 catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc agatccggcc actccacttt
    10801 acgcgtgcct ttcggggctt tgtaaccttg ccagaatgcc cagcatcgat ccgcatccca
    10861 gccttcaccg cttaactgaa catgcttgat gcccagaccg tcaatcttgc gcgccgcacg
    10921 ctggatcagc cccagatcgt ctgccccgtt caaatgcagg gtaatgccgt cattattaat
    10981 gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt tgggtagaga gggtaatctt
    11041 catcgcttct gtcattttag ttatccttct ttgtaaaagg gccgccagtt ggcagcccca
    11101 ggatttttcc gctaaatgat tcgcgttgca ggaaggcggc aagtgagtga agccccagga
    11161 gcatagataa ctatgtgact ggggtgaacg agcgcagcca acgcatctgc ggcgtgaagc
    11221 atgacgcgga aattattcgg cctcgtccag ccagactaac aaaatcgctt cgaggatttt
    11281 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
    11341 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
    11401 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
    11461 tggttgatcg tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
    11521 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
    11581 tctgagcttt ctggaagcga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
    11641 gcacaggatt tttcacaggc gtgttcaatc tcgataccgt tacgcagagc tgcgtcgaga
    11701 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagagatc ctgatgaggc
    11761 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
    11821 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
    11881 cgcttgttcg atggcgtcaa cgtcatcgcc ctgcgccact tcactcaggt gagcggcagc
    11941 atcgtcaatg acctgacgtt ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
    12001 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca ttcgggcttt
    12061 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
    12121 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
    12181 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
    12241 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
    12301 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
    12361 ggtgaaatcc tgagcgcggg ccactggaat agtggtatta cgcggaatca ctttctccac
    12421 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca acagcatttc
    12481 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
    12541 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
    12601 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt catcagcttc
    12661 tacacccgcg tctttcagcg cgcgacgaca agccaataag gttcgtttta ccagtggcgc
    12721 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
    12781 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gcggcatcca gcagttcacg
    12841 ctgaacgcgg ttatcgctac gatcaggaat gcccgcctgc tcgcgaatgt aatccgccag
    12901 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
    12961 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
    13021 ggcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cggtcggttc
    13081 gttaagtaag cgcaggacgt gaaggcccgc cagacgcgcc gcgtctttgg tgccctgacg
    13141 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
    13201 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
    13261 cagcagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
    13321 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgtcccatca ggcgtttaac
    13381 agaactaatt gtgttggcgg tatcgagcgc tgcattagta cgcgcgtcat aacccaccga
    13441 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
    13501 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
    13561 aataccggcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
    13621 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
    13681 cggcactgct tcgcagttta tcgagaaaac gcagcttacg cacggtatcc gccgccgcgt
    13741 cccaggtctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
    13801 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
    13861 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
    13921 cgctggcgag atcaaagccg tgcaaagaaa gcaaatattc cgcgcgcatt aacggatgac
    13981 gcagcgtttg ccaggcctga ttaatggttg cagattgctg tacggcggcg agttgttccg
    14041 cctggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
    14101 ggctcagcgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
    14161 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaacccc
    14221 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
    14281 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcgt tcaggccttc
    14341 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
    14401 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
    14461 tacataagcc atacctgaac atccggaggt tctcacgccc agacgtaggc caaacccttt
    14521 gccacggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctaa gtgtaatcga
    14581 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
    14641 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
    14701 cttcagcaat atcggtgttt ttgatagcct gcgcttcgtc gagagacttc cctttcaccc
    14761 attcggtgac cagggagctg gaagcgatag cagaaccgca gccgtaagtt ttaaaacgcg
    14821 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
    14881 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttgtcaaag gaacccacgt
    14941 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
    15001 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
    15061 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
    15121 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
    15181 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
    15241 acgtacaggc ggaaccggaa gaaaccgcga gatctttcag cgccataatc agcgactcac
    15301 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
    15361 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
    15421 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
    15481 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
    15541 tttgcgcttc gatgcgtacg cgcggtttac gacgcacata cagcgcaccg atacctttcg
    15601 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
    15661 taggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
    15721 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
    15781 tagaaacaag gatggtgtcg tcacgcatcg ctgcttcgag ttctttcagg tcgataatac
    15841 cgttacgctg cggtgcgagg taggtgactt cgaaaccttc gcgctccagc tgacggcagg
    15901 tatccaatac cgctttgtgt tcggttttgc tggtgattat gtgcttgcct tttttctgat
    15961 aaaagttggc tgcacctttg atcgccaggt tgtcagactc ggttgcacca gaggtaaaga
    16021 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
    16081 cttcttcagc ctgccagccg aaacggtgag aacgggaggc cgggttacca aaggttccgt
    16141 ccatcgtcat aaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
    16201 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
    16261 aggaaccagg ctaccggctg gatgtacgac cgtgtttacg gagcgcgata cgccccggcc
    16321 tgattctgaa tactttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
    16381 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttgttaa
    16441 ccagttcgcc taaagtaatg ttattgagaa aaccggtaag acggtcgctc aaatcacgcc
    16501 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
    16561 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
    16621 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
    16681 gcagacgaga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
    16741 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
    16801 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
    16861 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
    16921 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
    16981 ttttatcgtg gcgtttgtac ctgttatcct tccagaccat gggcgggttg agggcaatta
    17041 ttcgcattag cgcaggcctg ttcccctcac cctaaccctc tccccgaagg ggcgagggga
    17101 ccgtccgtgc acttatctgg cttagccctc aggccttatt acctttattc tgctgctcta
    17161 tagaagccag aatcccgcgc aggatattca attcctggct ttccgggcgc gcacgggtaa
    17221 acagacggcg caatttattc atcacctgcc ccggatggtt ttcacggata aaaccggttg
    17281 ccagcagcgt ttgttccaga tgaccataaa aacgctccag atcatcgacc agcggatacg
    17341 gcgtctcttc atgctcgacc tgctcgccat tttcctgagt tgccagccag gccatacgca
    17401 cttcataggc aataacctga accgccatcg ccaggttcag cgagctgtat tcagggttag
    17461 cggcaatcgc gacatgataa tgacacttct gcaactcttc attggtcagg ccgacacgtt
    17521 cgcgaccaaa caccagcgcc accggggtat ttgccgcttc agcaacgctt ttcagaccgc
    17581 attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga acgtgcgctg gtgcccacca
    17641 ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca
    17701 catcgctggc ccccgccgcc agggcaatcg cctgggagtc aggtttcacc agtggattaa
    17761 ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa cccatattgc
    17821 cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc tttcttcgtc
    17881 taaagattat tcgcgcatct tatcataaaa cgaagacaga tgccgatctc gctgctatac
    17941 tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat ccgatgctga
    18001 acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttgat tgccaaaaac tatgaaaccc
    18061 cggacgctgt agaagcgagc cagaaaggca gtaacgactt cgtgaccaac gtagataaag
    18121 ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac accatcatca
    18181 ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt atcgatccac
    18241 tggatggcac taccaacttt atcaaacgtc tgccgcactt cgcggtatct atcgccgttc
    18301 gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac gaactgttca
    18361 ccgccactcg cggtcagggc gcacagctga acggctaccg tctgcgcggc agcaccgctc
    18421 gcgatctcga cggtactatt ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca
    18481 ctacctacat caacatcgtc ggcaaactgt tcaacgaatg tgcagacttc cgtcgtaccg
    18541 gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa
    18601 tcggtctgcg cccgtgggac ttcgctgcag gcgagctgct tgttcgtgaa gcgggcggca
    18661 tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc gttgctggta
    18721 acccgcgtgt tgttaaagcc atgctggcga acatgcgtga cgagttaagc gacgctctga
    18781 agcgttaatg actcaggcgg gtgatatcac tcacccgccc tcgcctttca ggcgctattc
    18841 cgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct ttccccgatt
    18901 aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac aaacgcgttc
    18961 ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg
    19021 ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc tggtcggcgg
    19081 aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt attccttctt
    19141 cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc aatgccggaa
    19201 atatgtccgc ccactggccg ctggtcagtt ggttacccga gcgtaatttc aacgttttta
    19261 tgtttgatta tcgcgggttt ggtaaatcaa aaggcacgcc gtcccaggcc ggattgctgg
    19321 acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac ccacaacggc
    19381 tggtgctgtt cgggcagagt attggcgggg cgaatattct tgctgttatt ggtcagggtg
    19441 atcgtgaagg catacgtgca gtgatcctcg actccacatt tgcctcttat gcaaccatcg
    19501 ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc ggcgaaaatt
    19561 atatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagct gatcacgtta
    19621 tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa cggttgatcc
    19681 taatcccgga tggcgaacac attgatgctt tttccgatcg tcacggcgac gtttatcgcg
    19741 aacagatggt gaactttatc cttagcgcgt tgaatccgca gaactaaccc atgatcgcta
    19801 gcacgataat cattcacaaa atcaccttaa gacatgctaa tccactggtc aggacagttt
    19861 aagatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat tcccttcgcc
    19921 atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga tctacagatg
    19981 atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg ccagatcttg
    20041 ctgacgctct ttcagccggg gttgaccgcc accatggcaa ccttcagcga gcttaatggt
    20101 gtggatgatg atattgccag tcttgatatc agcgaaacag gacgggagat cctgcgctat
    20161 catcaactca cactgacgac tggttatgac ggtagctacc gggttgaagg tacagtgctt
    20221 aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg tccgtcattt
    20281 attaccagcc aattcacccc cgccctgaag agtgaactga agcggcgcgg aattgcgcgt
    20341 aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg acggctgcaa
    20401 aaacgctttg aatcgcgcga tattcatttc ctgtgtctgt atctgcaata ttgtttgctg
    20461 cagcaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc
    20521 tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag
    20581 cctgtaccac ctgatgagcc actgtttatg gcactacttt tttccatgtt gcgggttccc
    20641 gatccattgc gggatgcgca tcagcgggac agacaattgc gtcagtctat caaacgtctg
    20701 gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg gttatgtgat
    20761 cagctttata cccacctcgc ccaggcgtta aatcgcagtt tgtttgccat cggtattgat
    20821 aataccctgc cggaagagtt cgccagactg tatccacgcc tggtacgcac cacccgcgcg
    20881 gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga aagtggtctg
    20941 gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga aaaacagatt
    21001 attctactga ccgggaatga tagcgagcga gaagcgcaga ttgagcaaca gttacgcgaa
    21061 ctaacgttac tgccgctcaa cattaaacat atgtcggtaa aggcattttt gcagacaggc
    21121 gctccgcgtg gcgcggcact gattattgct ccttatacca tgccgttacc gctcttttca
    21181 ccaccgctga tctatacgga cctgacactg acaacacatc aacaggagca gatccgcaaa
    21241 atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg gaagcgccac
    21301 cagcgccatc acccagaaca cgccgtggcc cagatattga tacaggaaac cggcgaaaac
    21361 ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct gtaaacggat
    21421 gacttcgcta ccctggcgag cagcaatata gcgcatggcg gccaggtggc agaccgtgaa
    21481 ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag ttgctcccat
    21541 aatgccccag cgcactacgc cgcaaatcgc tgagatcaac agcatatcgc gtgcactaca
    21601 acggcggaaa agtttattac tcagtgcaaa gataatgact tccgccacca cgcccagcga
    21661 ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa tggcgctaaa
    21721 accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa agcgccagtt
    21781 ctggcgaacc agcgccaacc acgctgacca accggtgctc tcctgctggc ggcttgcccc
    21841 ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca cgcccaacgt
    21901 caacagcacg aggatcaccc gataatcaaa catagtgacc agtttgcccg tcagcgccga
    21961 gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa gcgggaactg
    22021 cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga aaaagaggtt
    22081 aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc
    22141 aaagagaagt gtcagcagtg ccagcacgcg caaggcggaa atcaggcgag aaggatcgct
    22201 gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg cccccaataa
    22261 caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc agaaaggtag
    22321 aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc gcgtggattg
    22381 caaaaccatg agtccctccc gtcaggaggc gttagtctgg agttaatcta atgttggtgc
    22441 aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag
    22501 cactcaaaaa ctgcgatccg tgacgcatgt tgcaaagata atgcgtaatt atgccgttac
    22561 gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccggct tcatctcccc
    22621 tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg gacgaaaaac
    22681 aacctgcccg gtattaaaat tattcatata accgggtatc aaagtgacgc ccagccccat
    22741 gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat ttggctggct
    22801 gttttcttgt gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat acgccggatc
    22861 ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt catgtgctaa
    22921 agggtgatca accggtaaca ccaccactaa tggttcgtca aaaagctcca gataatcaat
    22981 ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt
    23041 ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg gctgtctgag
    23101 acgaaacatc ggtaatactt ttggcagtaa attcacttcc gccgatggca caaagccaat
    23161 ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat tttccgcttg
    23221 ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca acgcgacttt
    23281 gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc ggatctggct
    23341 gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg
    23401 cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct tgttatcgaa
    23461 aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac cagcaacatc
    23521 cttatgacac aaaaatagaa ggtcaataca tcttatcttt caggattaaa aaatatgacc
    23581 acaccctcag atttgaacat ttaccaactg attgacaccc aaaacggtcg ggtcactccg
    23641 cgtatttata ccgacccgga catttaccaa ctggagcttg agcgtatttt cggtcgttgc
    23701 tggctatttc tcgcccacga aagccagatc ccaaaacccg gtgatttctt taacacctac
    23761 atgggagaag atgcagttgt cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc
    23821 aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgcggcaa cactcgcgcc
    23881 tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat cgatgtaccg
    23941 ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actgggggct aaacgaagtt
    24001 ccttgtgtgg agagttataa agggctgatt tttggcaact gggataccag cgcaccgggc
    24061 ctgcgtgatt acctgggtga cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa
    24121 ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgcaa ctggaaattc
    24181 ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc ttctgccgtt
    24241 caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc
    24301 cgcccggtgt gggaaaccgc caaagatgcc ctgcaatttg gtcaggatgg tcacggcagc
    24361 ggcttcttct ttactgaaaa accagatgct aatgtctggg tcgatggcgc agtttccagc
    24421 tattaccgcg aaacctatgc cgaagctgaa caacgtttag gtgaagttcg cgccctgcgc
    24481 ctggcgggtc ataacaatat tttcccgaca ctttcctggc tcaacggcac tgccacactc
    24541 cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg tattactgac
    24601 aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg tgcttttggt
    24661 cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca gaaattgctt
    24721 aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag
    24781 cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac tgctgctcgt
    24841 ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa gctggcagga agtgctcgat
    24901 aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca ctagagttac
    24961 atcaccgcat tagccagttt ctctttcacg aagccagctt actggacgac tggaaatttc
    25021 gtgactggct ggcacagctc gacgaagaga tttgttacac catgcgcacc acggtgaacg
    25081 cgcaaacgcg cgaccgccgc aaaggtgttc agccgccgac aacctggatt tttaatgaca
    25141 ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcatggca tgggcagaag
    25201 agccgccgtc acgcacccgc cacttaatca gcaactgcca gataagcgaa accgacatcc
    25261 caaacgtatt tgctgttcgg gtgaattatc tgctttatcg ggcacaaaaa gagcgcgatg
    25321 aaacgttcta tgtcgggact cgttttgaca aagttcgccg tctggaagat gacaactggc
    25381 gcttgctgga acgggatatc gtcctggatc aagcggtaat cacttcccat aacctgagtg
    25441 tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc
    25501 tctccggatc gatacctcgc ccgtcatcgc cttgttcaac gttggcggcg agttttatgc
    25561 cattaacgat cgttgcagcc atggtaatgc gtcgatgtca gaagggtatc tggaagatga
    25621 cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt
    25681 atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag gtggtgacat
    25741 tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc catttttatt
    25801 accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga agaaggcgcg
    25861 caggttgcca cgctggaact gtcggcggcg aaagtcgcca gtctgcgtca gcgatttggt
    25921 gaacatattc tggcggtgga aggcaacgtg acctgttatg ccgattatca acgcgcggtc
    25981 gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc gggcatctgg
    26041 gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg cttccacgag
    26101 ctgtttaacg tcaacgtact cggttacctg ctgggtgcaa aagcctgcgc tccggcgtta
    26161 atcgccggtg aaggcagcat gattttcaca ctatcaaatg ccgcctggta tcccggcggc
    26221 ggtggcccgc tgtacaccgc cagtaaacat gccgcaaccg ggcttattcg ccaactggct
    26281 tatgaactgg ctccgaaagt acgggtgaat ggcgtcggcc cttgtggtat ggccagcgac
    26341 ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcaa taatgcagtc tctgacgccg
    26401 gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggctga ttttacgggt
    26461 ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac
    26521 gctgatgcgg gtttagcaat tcgcggcatt cgccacgtag cggctgggtt ggatctttaa
    26581 ggaagtacga tgaaagaaaa aacgatcatt attgtcgggg gcgggcaagc ggcggcaatg
    26641 gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt ttccgatgag
    26701 caacatcttc cttatgaacg ccctccgctc tcgaaatcca tgttgctgga agattcccca
    26761 cagttgcagt ctgtgttacc cgctcactgg tggcaggaaa acaatgttca tctgcattcc
    26821 ggtgtaacca tcaaaacatt gggccgcgac acacgagagt tagtgttagc caacggcgaa
    26881 agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc gctgccgttg
    26941 cttgatgcac tgggagaacg ctgctttacc ctgcgccatg ccggtgatgc cgccagactg
    27001 cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac tattggtctg
    27061 gaactggctg ccagcgccac gcagcgcaga tgtaaggtga cagtgattga actggcggca
    27121 accgtcatgg gccgtaatgc accaccgccc gtgcaacgct atcttttaca gcgccatcag
    27181 caggctggtg tgcgcattct gctcaataat gccattgaac atgtggtcga tggtgaaaaa
    27241 gtagaactga cgctgcaaag tggcgagacg cttcaggccg atgtggtgat ttacggtatt
    27301 ggtatcagcg ccaacgacca actggctcgc gaggccaacc ttgatactac caatggcatt
    27361 gtcattgatg aggcttgccg cacctgcgat cccgcgatct ttgccggtgg cgatgtggca
    27421 atcactcgtc ttgataatgg tgcactacac cgctgcgaaa gctgggaaaa cgccaataac
    27481 catgcgcaaa ttgccgctgc cgcaatgtta gggctaccgc ttccgctact gccgccgccg
    27541 tggttctgga gtgatcagta cagtgataac ttacagttta ttggcgatat gcgtggcgat
    27601 gactggcttt gtcgtggcaa cccggaaacc cagaaagcga tttggtttaa tctgcaaaac
    27661 ggcgtgctta tcggtgcggt aacgctgaat cagggacgtg agattcgctc aatccgcaaa
    27721 tggatccaga gcggcaaaac gtttgatgcg aaacagctga cagatgagaa catcgcgctt
    27781 aaatcactgt aaccaggata attagcgaat atctcaacgc ctggggcgtg gcgaggtacc
    27841 agagtgtgta ttacgtttaa atcacattat cttgcaaagg gattggttat gaactcacta
    27901 cgttattttg attttggtgc tgcccgcccc gttttgttat taattgcccg tatcgccgtg
    27961 gtcctaattt tcattatttt tggttttccc aaaatgatgg gctttgacgg tacggtccaa
    28021 tatatggcct cgctgggcgc accgatgccg atgctggcag cgattattgc ggtagttatg
    28081 gaagtgcccg ccgcgatatt aatcgtgctt ggctttttca cccgtccgct ggcggtactg
    28141 tttattttct acacgctggg tacggcggtg attggtcacc attactggga tatgaccggc
    28201 gatgccgtcg ggccaaatat gattaatttc tggaagaatg tcagtatcgc tggcgcgttc
    28261 ttgctattgg caattaccgg gccgggggca atttctctcg atcgccgtta ggaaagatgc
    28321 cggatgcggc gtgaacgcct tatccggcat tttataaatt acagccactc tacccgcaac
    28381 gacatctctg aggttgttga ttcccccggc gcaagcgcaa tgaggtcacc cccttccggg
    28441 cgatgatggt catccggcgc atggctcatc ggctccagac agaagaaatc aaacgcatat
    28501 cctttatcaa acgccgggtc ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt
    28561 tctatgatga tggcataacc ctcctgcggc tgctcgatgc gggcctgacc attccatccg
    28621 gcaaaaccat tgttcaccca ctggcgcggc aacggcgcta gctggttaaa atccagttct
    28681 tgcggtagct gctcgcaaaa ctcacccgcc agccactgct cccgctccag ccagtaaccg
    28741 ctcgcctgtg cctgaatccg cgtttgcggc gacaacggaa aataaggatg ccagccggta
    28801 ccaaatggca gcgtctctgc cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc
    28861 gccgttaaat gaaacgcctg acttacccga tagtgataga caccgctgcg atgttcatac
    28921 accagacaca aactatcatc gctgtgcgag acacattgcc actcgcccag ccagccatcg
    28981 ccgtgtagat agtgcgcatc ccactcaaca ttcggttgca gttgatactc acgcccctgc
    29041 cagacaaaac ggttgccgct cacccggttt gcaaatggca ccagcggaaa acaagaggca
    29101 tcggttgcca caccgctttt tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag
    29161 ccttcgatta cgccgccctg gtcagaaacg cccagcttta gcgacccgtg ggataaggta
    29221 tagatggtca tccgctaact ccttaatccg ggaagttaat cacaactttc ccgcatttgc
    29281 cgctcgccat cagcgcatag gcatcacctg cctgttccag cgagaagcga tgggtaatgg
    29341 cgttacgcgg ccacagcttc cagtcagtca aatcgtgggc gcatttttcc atatggaaca
    29401 gactggtcac ccaggagcca ataatccgcc gttgatggtg catcagatcg gcgctgacct
    29461 cgaattccac ttttccggtt tcaccaatgt aaaccacccg cccccagtca gcggtggatt
    29521 gcagtgccag taagcgacct gcggcattac cggaacaatc aagcgcaaca tccgcgccgc
    29581 cgtgagtgag ttcggcgatt atctgcggca gaccttcagt ggtcgctaaa tagccgtgat
    29641 ccatcactcc taactgtttt gccatcgcca gacgttccgg cagcatatca acgccgataa
    29701 tccgttttgc accgcgacct ttcgccagca tcatccccat catgccgact ggccccagac
    29761 cgaccaccag cacgttatca ctgccggaaa cttcgccgcg caaaatccct tcataggccg
    29821 tgccaacgcc gcaactgata aacgcaccat cttcgtagct cagcgcatcc ggcaggagga
    29881 tcagatcttt ttcttccgcc agcaagtatt cggcatgacc gccgtcacgc tgccagccgt
    29941 aagccgcttt tccttcgcca gtacaagaaa taggaaaacc gcgacggcag ttcgggcaaa
    30001 aaccacagcc agaaatgtga tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc
    30061 cttgccccat cgccacaatc tgcccgcacg gttcatgacc gttgataaat ccctagtaca
    30121 acggtttatc ggatgccgcc gccgtagcgc ggtgctggtg gtagatatag tggacatcgc
    30181 ttccgcaaat tccggaggat ttcattttga tcagtacctg gttaatcccc ggcgtcggca
    30241 ctgcaacttc ccgcagatcg acggtcgaat ttcctggtaa ataagctgcc agcatcgttt
    30301 tcataaatcc ctcattaaca atacgattaa ttttcatccc tgccgcaccc gcgccagggg
    30361 cgttaaggtt tagcgtttcg ctttactgct acgctgggtc agcaagatat tcgccagcac
    30421 cgccaccacg atgatgacgc cgcgcaccac ctgctggaaa aaggagttaa tcccgagcag
    30481 caccagaccg ttaccgatta gcgtaatcac cagtacgcca agcagcgtac cgaacaacga
    30541 gccgcgacca ccggaaagcg ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc
    30601 cagaccgttt gcggcacctg cgttaccgga accgaggcgc gccgccaaca aaatgccggt
    30661 caccgccgct aataatcccg aaagggtaaa gataagaatg cgtacccgac gaacgttaat
    30721 accgcacaac tgcgccgccg tggcattacc gccaacggca aaaaccgagc gcccgaaggc
    30781 ggttttgcgg ctaatgaaca caaacagcgc aaacagcacc atcatgatca gcgcggatac
    30841 cggcacaccg agaaattgtc cgcccagcca gtccagcacc tcgttttcgt caatcggcac
    30901 tggcagagcg ttggtcataa acaagcccat tccgcgcagg gcgctccaca gccccagcgt
    30961 ggccacgaaa cttggcacgt taaacacgcc acgcagcacc ccggcgagcg ttcccatcag
    31021 cgcgcctaac agcaacacca gcagacaagc aatcgccaac ggaacttcaa attgcagcaa
    31081 aaatgccagg cataccgaga caaaagccac catcggccca acgctgacat caatttcacc
    31141 ggagataata atcagcgtca tcgcccaggc ggcaatccca atggtggcgg catcgcgcag
    31201 cacgttcatc tggttattca atgagataaa gccaggcgcg ttcagggaga agaccagata
    31261 aagaatggca atcaccacca gcaaaccgat ctcattaata tggtgactga caaattgttt
    31321 gagcgagacg ctcttgccct gcggcaatgg taatgacgaa gcagacatct tcaggttcct
    31381 cggtaaaatt catcagtgca cagacagaat ggcggacatc agctcatcca cattgaccgg
    31441 agcgtgaaac tcctgcgaga acgtgccgtg ctgtaataac aggatgcggt cacacaccaa
    31501 cggcaactcc tccacttcac tggagataaa caccacgctt tttccttcgg cagccagctc
    31561 acggacaata cggtaaatct gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc
    31621 gagcaacaaa atctggctgg cggcatagac ccaacgaccg atcactactt tttgctgatt
    31681 gccgccagaa agcgtgccga tgggcgtttc gctactggcg gccttgaccg tcatccgctg
    31741 catcacttct tcggtcaggc ggcggatggt ggaccattgc agcactccgt tggcgctgat
    31801 tttttgccga ttggtcagca ctgtattttc gtcaacgccc aaccagggaa tgatccccgc
    31861 ttctttgcgg ttttctggcg tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg
    31921 gcgcgtgatt ttctcgccgt tgataacaat ttcgccctgt tcatacgtct ccagcccaac
    31981 aatcgccttc agcaattcac tgcgccctgc ccccagcaga ccagcaatgc cgagcacttc
    32041 gccacgacgt agcgtaaagc tgatatcctc cagcttgggc ttatggcgta acgcacggac
    32101 ttccagcacg gcctgatcca caatttcctg aggtgcaacc ggcgcaatat caacgtgatc
    32161 gcgccctagc attagcgaca caatatgatg cgtggaagtg ttttcgagca tcacatcgcc
    32221 agccacctgc ccgtcgcgca taacggtggc actggaggca atgcggcgaa tttcttccat
    32281 ccggtggctg acataaatca ccgccacgcc cagtgctgac atctttttca ccgctctgat
    32341 caccagttca acttccgcac tcgcaagcga actggtaggt tcatcaagaa tgaccacgcg
    32401 cggctcgccc ttcatcaccc gcgcaatttc caccagctgc ttatgcgccg ggcttagtgt
    32461 tgaaacaagt tgttcaggac taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc
    32521 ctgcgccatt tgcaggtaat caatcatgcc gttgcggcgg ggccactgac cgaggcagag
    32581 gttttccgcc accgtcagcc cttccaccag acttaactcc tgataaaccg cgcgtacccc
    32641 cagttcagcg gcacggcgag tcagcgtagc ttcgtcacct tccagtcgcg tctcgccaat
    32701 ccagatttca ccgctatccg gacgttcact gccggtaagc attcgaatga gggtcgattt
    32761 gcccgcgccg tttttgccta acagcgcacg aacctcgcct ttattgagcg tgaagttaac
    32821 gttatccaac gcaacgacgc cgggataacg cttatttcct gccaccactt ttgctaccgg
    32881 gactgcctct gttgccgtga acatagggac ctctgcgaat cagcgattag ggcagaccat
    32941 caacgtgcgt tgccagccac tgtttaccgt cttccgtttt ggtatagaga tcgataggca
    33001 cctgaatcac tttttcaccg tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa
    33061 cagcattgcc cattttctta ccggaaatat ccactaccgc tttcagcacc tgattgtttt
    33121 ccagctcctg agcaatttcg gttgtcatat ccgaaccgaa aacagcaatt tttccggcct
    33181 gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc accgcccgat tcccccataa
    33241 tggcgttgag atccggcgtg gagataatca gtttttcacc aacggaaatc gctttatcta
    33301 aaacagtccc ttcctgatta gcgacaattt gcgcgccggg aacgcgggat tttaatactt
    33361 cttcaaatcc tttacgtcgc tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa
    33421 ttttcggctg gtcaatttta ttggcaataa aataatcggc ggcagcgtta cccagttttt
    33481 taccaaattc cagcgggtca cccaccagat acgccgagac atatttatcg accccctttt
    33541 gattaataca ggtgttgtag caaatcaccg gaatacccgc ttcactggcg cgacgaacgg
    33601 tacggctact gccattttca gacactgccg ataaaataat agcatcgaca ttacgcgcca
    33661 cgagggtatc aacaaaggta ctttctttcg aaatatcacc ctgggcgtta gtttcaatta
    33721 actgtacctg tactgaagaa tcttttgccg catcctgaac tccctgacgc actccagcgt
    33781 aatatccctg agtatcaagg tatattgcgc caatggtcat ttctttatcc gcagctctgg
    33841 caaatagtgc gctacctaat agcgtagcca ttaataatag attacgggtt gttctcattt
    33901 ttttaggcat agagcctcct gtagggtttt tattaacaac ggcttattct aattattttg
    33961 tgatgagcgg cagcgcgtgc cgctcaaata ttacagttaa tagggtttag tgaacgtagt
    34021 taaaaataaa aggcatcacg gtggtgaata atgccgcttt cggccaggcc ggattaatcg
    34081 ccgtcaattc agcgcgagca gattcgtatt gcgcaatctc gcctaatccc gcggccgcca
    34141 gcatcgccac catcaggcat ttttctttat gctgctgttg taaatcgcca tacagcgaca
    34201 acaagtcagg ctgcgagacg gcaaagaaat ccgcttcgat actggttttc gccatctctt
    34261 gcgcccactg tttcatttca ctaaacagtt gctgtgcggt gtgttgttcg cccagtaatc
    34321 gcagcgccat tccttgccag aagaggtaat caaccggctg atcgttgtaa tagctgtgga
    34381 tgttaatggt gcgatcgccg gtcgccgcca gatgtaaaca gcgcgtcgct tcagtttcat
    34441 cgccctgggc tttggcgcat atcgcctgcc agaaccagat gtcgttatca gtttgccccg
    34501 gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc ggcatgaagc agttcgctgg
    34561 cctgctgcgg ctctctggca tcaagatgct gccaggcgcg taataactgg ttgaggataa
    34621 actgactggt gaccttccct tccccgcctt cccacgggtg gaatttgcgc gtggcgagaa
    34681 tgtccgccgc tttgtctgcc tgccccgtga gatgccacaa attgagcagt tctgcggtca
    34741 tgtcgtcgcg tttcagcgca atttccagat tattttccag acgcgccagt cgtttctccg
    34801 gtgtggttcc acttagctta tcaagcaaat cccgttcgaa aagcagacgt gcatcctgcg
    34861 gcgcaagctg ataagcatta tcaagataac gcgcggccag ctcataatcg tgttgcttat
    34921 tccacgcatg gatcgctaac ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc
    34981 aacgttgcca taaggcaatg gcttcgttgt agctacgttt gttgtagtag aagcaggcca
    35041 gtaaatggcg agcaaaccag cactcttcaa tactctccag cgccgccact tcttccagcg
    35101 tattcgggaa acggacaaac tgcgggaaga catcaatggc ttttgcgacc agttcgccac
    35161 gttcggcttt cggcagcaag ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca
    35221 gtgcattcag catctctgcc gcgagggtgg gcattcccca gttaatcagt tgcccggcgg
    35281 tcatcagggc gttaacgtcg cgtccctgac acagaccgcg ccactgcgcg agagctgatt
    35341 cgctacgacc atcgaaccag ttcaggcacc acagagtggc gttcagcgga taatcgcgca
    35401 gcagtttctc gcgctgcaaa cgcgcgttgt cctgacgacc acttaacacc agcagcagat
    35461 tatgcaggca aagcacttcc tgattgattg ggcaggcgcg aagactttgt tggcaaaaat
    35521 ccagacccgc gtcgaagtta ccattacgcg ccgccagtcg tgccagacca taatagcctc
    35581 cggctttact gttgccgctc cagaccgcac gccagaaatc ctcttcggct tgttgatatt
    35641 gtccctgacg ttcgtaagca ctggcgcgaa tcaaactcgc ctgtccgcac tgcggatttt
    35701 tgttcagcgc atgtgcgcgt ttcagagcct gagtggcata cgccaccgct tgcgggaaat
    35761 ctgcgcggtt atactccagc atcgccagcg ccagattaca gcggtaatcc agcggatcca
    35821 gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct ggcgtgatga tattgctcca
    35881 gatgctgacc gataaaccag gcttcatctg tactggtaat gtcttgtgct gacagtggcg
    35941 ctttggcgac gtccggcaac ggcaactctt gcggctgatg ttcctgataa ctcagtacaa
    36001 tattgccgtc ggcatcagag agctcaatcg tcagtctttc aggattgata ccgtgtaaca
    36061 cgccctggat ggcggtcgct ggtgtcaggg ccacggcatc atcgagcaac gcgtcgcatt
    36121 tgccgatttc gcggatcgcc aggcggtatc cgttcaacgg agagatggca tacagccccc
    36181 actcaatccc ccgctcacta cgctggagtt tgattaccgc atcgcgggag gcgttttgca
    36241 ccatgcccag agaatggtaa ggcaggaaaa actgctcaaa acgcttctct tcgtaagcat
    36301 caagccgggt aaaatcaggc tggttatcgg caaaaatacc ggtcatcagt tcgatatacg
    36361 ggccgttatt atcagttagg ctcttatccc acgcctggcc aaattcactg tgtccccagc
    36421 tccactgctt tttacctggc gcaatatggt ggttggcaac gtgtagcaaa ccgccatctt
    36481 catcgtgaca ccacgcgcca acaaaatcgt actgtgattt ttcagccata tatgaggttg
    36541 gaacgggcac atttttatag cgagaaatgt ccactccagc ggagtagtcc actttgtagt
    36601 aagtgccggt ggcgataggg aaagcggaga cggcccgttt gccgtgatca aacaccgccg
    36661 ttacatccgg cggaaagacg ctctgatgcc cttcaccccc tttcactgcc gggttggccc
    36721 accacaagaa atgacgcggc gtggcgttgc cgttatagac gcggctggcg atttccagcg
    36781 ccgcccggtc agggcgcagg gtgaaacctg tcatcacctg taaaccatgc atcggctccg
    36841 tttcgcctac ccacaccgtc tgtgcgccgt cttcatgggc ttcgagggtg aaatcaacgg
    36901 gcataaaggt ggtcgggcga tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc
    36961 acggtcccag cagccccacc agcgcaggtt taatgacttc attgtgatag acaaaatcgc
    37021 gctgtttcac tttatcccat gcgcgatgca cccgaccgcc caattccggc aggatcatca
    37081 ctttgatgta gtcgttttcc agccacaccg cctgccagga tttcagggtt ttctgctcgc
    37141 tcagcgtatc ggtcacgccg tagggataaa ccgcgccgga cgatccctga taaacgcgat
    37201 tttccaggaa catgggatgt atatcctgcg gcccggtttc ataggtcggg atctcaacgc
    37261 gctcttgcca cacttttact ggagtcatgg tgccctcaat attaacaaga catactgaat
    37321 taaaagattt gtggcagtgt attgaacaat ctggcaatgt tttcgcggaa taatcacgca
    37381 attaactaaa caaggtttag tgaagatgag agcctgcatt aacaatcaac agattcgcca
    37441 ccataacaaa tgcgtgattc tggaactgct gtaccggcaa aagcgcgcca ataaatcaac
    37501 gctggcccgg ctggcgcaaa tttcgattcc ggcagtcagt aatattttgc aggaactgga
    37561 aagcgaaaaa cgggtggtga atatcgacga tgaaagccag acgcgcgggc atagtagcgg
    37621 tacatggctg attgcgccgg aaggtgactg gacgctgtgt ctgaacgtga cgcccaccag
    37681 tattgagtgt caggttgcta atgcttgttt aagtccgaaa ggtgaatttg agtatttaca
    37741 gattgatgca ccgacgccgc aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca
    37801 ccgtaaattg tggccggacc gcaccatcaa tctggcgctg gcaatccacg gtcaggttga
    37861 tccagtgact ggagtgtcgc aaaccatgcc gcaagcgccg tggacaacgc cggttgaggt
    37921 gaagtatctg ctggaagaga agctgggcat tcgggtgatg gtcgataatg actgcgtgat
    37981 gctggcgctg gcggaaaaat ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa
    38041 cgttgattac ggcattggct cgtcgttcgt gattaacgag caaatttatc gcggcagttt
    38101 gtatggtagc ggacagattg gtcacaccat cgttaatccg gatggcgtcg tctgcgactg
    38161 tggacgttac ggctgcctgg aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg
    38221 ggtatggcta aaatcacaac cggttaatac tcagcttgat cctgaaaaac tgactacagc
    38281 gcagttaatc gctgcctggc aaagtggaga accgtggatc accagctggg ttgatcgctc
    38341 tgccaatgcc attggtttga gtctgtataa cttcctcaac atcctcaata ttaatcagat
    38401 ttggttgtac ggtcgcagtt gtgcctttgg tgagcactgg cttaatacta ttattcgcca
    38461 gacaggattt aacccgttcg accgcgacga aggaccgagc gtgaaagcga cgcaaattgg
    38521 cttcgggcaa ttaagccgcg cacaacaggt gctgggaatt ggctatttgt atgttgagac
    38581 gcagttacga cagatttgat ggcgcgataa cgtagaaagg cttcccgaag gaagcctttt
    38641 tcaacagatt aaccgtttcg cttatgcgta aaccgggtaa cgtgcgcaga tgtcgagaac
    38701 tttacctttg atgcgctcga taacggcttc atcattgatg ctgtccagca cgtcacacat
    38761 ccagccagcc agttctttcg cttctacttc tttgaagccg cgacgcgtaa ttgccggagt
    38821 acccacgcga ataccggagg tcacaaacgg gctcttcgga tcgttcggta cgctgttttt
    38881 gttgacggtg atgttagcac ggcccagagc ggcgtctgct tctttaccgg tcaggttttt
    38941 atcgaccaga tcaaccagga acagatggtt atcagtaccg ccggaaacca ctttgtagcc
    39001 gcgttccagg aacacttcta ccatcgcttt agcgttttta gcgacctgct gctggtaagt
    39061 tttgaactca ggctccatcg cttctttcag agcaaccgct ttaccggcga ttacgtgcat
    39121 caacggaccg ccctgaccac cagggaaaac ggcagagttc agttttttgt acagctcttc
    39181 gctaccacct ttcgccagga tcagaccgcc gcgcggaccc gccagggttt tgtgagtggt
    39241 ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag acgccagcag caaccaggcc
    39301 cgcaacgtgc gccatatcaa cgaacaggta agcgccgatg ctgtcagcga tttcacgcat
    39361 tttcgcccag tccaccacgc cggaatatgc agagaagcca ccgataatca ttttcggttt
    39421 gtgttctttg gcttgttttt ccagatcggc gtagtcgata tgaccggtag catcaatacc
    39481 gtaaggaaca atgttgtaca gtttaccgga gaagttaacc ggagaaccgt gagtcaggtg
    39541 accgccatgc gccaggttca tacccagaac ggtatcacct ggttccagca gcgcggtgta
    39601 gaccgcaaag ttagcctggg agccggagtg cggctggacg ttagcgtagt cagcgccgaa
    39661 cagttctttc gcacgatcga tcgccagttg ttcaacgata tcaacatact cgcaaccacc
    39721 gtagtagcgt ttgcccggat aaccttcagc atatttgttg gtcagctgag aaccctgcgc
    39781 ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc agttcgatgt gctcttcctg
    39841 acgtactttt tcctgctcca tagcctgcca cagttcggca tcataatcgg caatgttcat
    39901 ttcacgcttt aacatccgca tctcctgact cagctaacaa taaaattttt ggcctttata
    39961 ggcggtcctg ttggacaacg gcgaacagta taaccgaatc attgtgcgat aacaggtctt
    40021 gacaaaggaa tttacgcaaa cgattacctt caggctacgc aaggctttgg agaataaaga
    40081 gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg caaatttttc acttcatcac
    40141 attctttctg aaaaacacca aagaaccatt tacattgcag ggctattttt tataagatgc
    40201 atttgagata catcaattaa gatgcaaaaa aaggaagacc atatgcttga cgctcaaacc
    40261 atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc
    40321 catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt
    40381 aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt
    40441 aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc
    40501 agtttccaga tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg
    40561 gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta
    40621 ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc
    40681 ggtggctggg aaggtactcg cgatttccgc attgtggcta aaacacctcg cagcgcgctt
    40741 atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg
    40801 caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac
    40861 tctttgactc gcaaaccgga tggcaaaggc tatcgtattg cggtgaaacg cgaagagggt
    40921 gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc
    40981 gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct
    41041 gccggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac
    41101 acagcacaag tgaactggtt ccatgcggca gaaaatggcg atgttcacgc ctttgccgat
    41161 gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag
    41221 ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc
    41281 aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc
    41341 ttcatgcagt ttgccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat
    41401 tacgaatgct ttggcccgca taaggtgctg tgatttgatg ttgccggatg gaaacatccg
    41461 gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg
    41521 acccgtgcca cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg
    41581 cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc
    41641 actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc
    41701 tggcggccaa aacctttcac ctcagtgacc gtcatgccgg taatgccgac ttcagccagt
    41761 gcttcgcgga catcgtccag cttgaagggt ttaataatcg catcaatctt tttcatgcta
    41821 ttccttgaaa aggtcgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat
    41881 tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg
    41941 ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt tggtaatttg cagcagctta
    42001 cgcaaatagt tgagttcaaa ctgattacgt gcctcaacaa aggttggcag cgccgtattt
    42061 tcaccctcca gcgcctgctc tactaatgca tcgctaatca ccggagatga ggtcagcgcc
    42121 acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc
    42181 aggcgtttca tcgcatcggt ggaaaacgcg cggacaaacg gtttatgtcg ctctgccgcc
    42241 tggcgcaaca ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga
    42301 attttcaggc tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc
    42361 atcgcttttg gcagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg
    42421 cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcaacag tttgacctgc
    42481 aacggcgcag gcatatcgcc aatctcatcg agaaatagcg ttccgccttc cgccgcctgg
    42541 aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc
    42601 tccgactcca gcaattgctc aggcaatgca ccacagttaa tagcaataaa tggtttgcta
    42661 ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctgg
    42721 ccattaatca aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc
    42781 agcatcagcg ggctgcgggt gacaatcgcc tcgcgccagc gttcgtcggt ggctggcgcg
    42841 gattgttcca gcgcatcatc aattgcctga tatagcgcgt ctttatcaac aggcttggtg
    42901 aggaaactaa aaacgccctg ctgtgttgca gcaacggcat cgggaataga accatgcgcg
    42961 gtaagaataa ttaccggcat tcccggctgc actttctgga tttcagcaaa cagctgcata
    43021 ccgtccattt catccatccg caggtcgctg atgactaaat ctactttttc gcgattcagt
    43081 acccgtaatc cttcagcgcc actttccgcc gtgaccacgc tgtagccttc gctggtcagg
    43141 cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt
    43201 ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgtgg
    43261 cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcagt
    43321 caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg
    43381 gcgcaatgta tccagctcgc tgtcgctgga ctgctggagt ttactgtagc gttggcgctc
    43441 ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac
    43501 ctgtgccggg atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata
    43561 tggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc
    43621 gtcgtcatat tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc
    43681 catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat
    43741 gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc
    43801 tggcgtatca atggctggct tattgtgatt ctgcacgcaa cctagcaatg ccagacatgg
    43861 cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac
    43921 cagacttaaa gcctatccca gtgggcgtaa ttgttgcaga cagtttggac atggacagcg
    43981 cggagaaacc ggagcgtaca tgtcgtacgt gaggatttcg agcactgccc ggggccgaac
    44041 tgacaaataa aatagcctga tgggataggc tgttagcatt atttcgtgtt tttcgacgac
    44101 ggtaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct
    44161 tgcatacggc gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc
    44221 ccttttcgct ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc
    44281 ggaatgggcg tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg
    44341 cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc
    44401 agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg
    44461 gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaactactgt ctccaccagc
    44521 ggtgctaact caacattatc tagttccacc gcactgtccg cctgtttacg gttgtaatca
    44581 agcagttgtt cgatcagttt ttgcaaattg cggctgctgc tatcaagaat gctcaccacc
    44641 tctttttgct ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg
    44701 cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt
    44761 tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt
    44821 aactcgctcg gtccactgaa cgaaacgcta ttgcccagag aacgcccttc ccccaaccga
    44881 ttgatcatcc gttcaatatt tttcaccggc ccgataatca tccgcgtgaa aagcagtacc
    44941 attaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg
    45001 gcgatttcac gctgaagttg ctgcccacga gagaacacta ctgtgcgcgt ggcctgtacc
    45061 atttcggtat tggcactggc aaaggcttcc agacgcgcgg cagcagcagc atcgggacca
    45121 ctgttgttgc actgaagttg ggccagattg ttcaagtcct gacgcaatgc ctggtaaagt
    45181 ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg
    45241 ctttgataaa ccttcgcaag cgtagggtca tccagcacgc aatactgacg gtagctacgc
    45301 tccatctcca gcgccgcgtt ggtcattgct tcgctgcgcc gggcatcgat aagcgtggtg
    45361 cggttgacca gcgccgcctg gtcgctaagc gcattcaggc tttgccaggc ttgccatgcc
    45421 agcaccaaca ggggcagcag aatcagcaaa aatgccagca ttaccagttg tcgtaatgag
    45481 cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg
    45541 ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat
    45601 aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa
    45661 gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg
    45721 gctgacataa gtcggtgaat gagccactga ttactattat gcagtaactg tgccaataaa
    45781 gaaaatagtt tggtaacgta ctgattatac gttgctttga ggggtttatg gcttccccgc
    45841 tatttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga aaaacttatt
    45901 agattacaat aaaatcaagc atttaagtgt cgccagaaag cgacacggta aaccacccat
    45961 tgtcgtgatt tacagacaca aaaaagctcc cgaagttggg agcttataat agcggttgta
    46021 tttaatgccg gatgcggcgt gaatgcctca tccgggctgc aaaaccaatg agtttgacgg
    46081 cttaccccaa ctgcttacgt gcattgcgga aaatgcgcat ccatgggcca tcctcgcccc
    46141 agttttcagg atgccaggag ttgctgacag tacggaaaac acgttccggg tgcggcatca
    46201 taatggtgac tcgaccgctt tcagtcgtga ccgccgtaat accgttcgga gaaccgttcg
    46261 ggttagccgg gtaggtttca gtgactttgc cgaagttatc gacatagcgc agtgccacca
    46321 gccctttgct ttccagtgcc gccagatgcg ccgcatcacg cacttccacg cgcccttcac
    46381 catgagagac agcaatcggc atttgcgagc ccaccatccc ctgcaacagc agcgacgggc
    46441 tttgggtcac ttcaaccaag ctgaaacgcg cttcaaagcg atcggaggta ttgcgcacaa
    46501 aacgtggcca caactcacta cctgagatca gttcacgcag attagacatc atctggcaac
    46561 cgttacatac ccccagcgcc agcgtttgcg gacggtggaa gaaggttgca aactcatcgc
    46621 gtacacggtc attgaacagg atagacttcg cccaaccttc accggcaccc agcacatcac
    46681 cgtaggagaa accaccgcac gcgaccaggg cgtggaaatc ctccaggccc gtgcgtccag
    46741 ccagcaggtc gctcatatgc acgtcgatag catcgaagcc tgcacggtgg aaagctgccg
    46801 ccatttcaac atgcgagttc acgccctgct cacgcagcac agcaactttc ggacgtgcgc
    46861 cagtggcaat atacggtgct gccacatctt cgttgatatc aaacgacagt ttcacattca
    46921 ggcccggatc ggcgtcgttt gatttcgcct gatgctcctg atcggcacac tccgggttgt
    46981 cacgcaggcg ctgcatctgc caggtagttt ctgcccacca gacacgcaac gtagtgcggc
    47041 tttcgctgaa tacagtctgc ccgttggcgg taatcacaaa acggtcaccg gaaaccgcct
    47101 gcccaacata atggacgcaa tcagcaagcc catgctgtgc cagtacggac tcgaccgctt
    47161 cacggtcagc ggcacgaacc tgaatgaccg cacccagttc ttcgttaaac aacgccgcca
    47221 ggcgatcgtc acccagagtg gcgatatccg cgtcaatgcc gcaatgacca gcaaaggcca
    47281 tttccgccag cgttaccagc agaccgccat cagagcggtc gtgatacgcc agcaacttac
    47341 gctgtgcaac cagcgcctga atcgcgtcat agaagccttt cagttgcgcg acatcgcgta
    47401 catctgccgg tttgtcgcca agctgacgat aaacctgcgc cagcgccgtt gcgcccagcg
    47461 cgttattgcc tttgcccaaa tcaatcagca gcagtgcgtt atcttcggta gaaagctgcg
    47521 gcgtgatggt gtgacgtaca tcttccacgc gggcaaatgc agaaatcacc agcgacagcg
    47581 gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg ggttttcatc gacatagagt
    47641 ctttacccac cgggatcgtc aggcccagcg ccggacaaag ctcttcgccc acggctttaa
    47701 cggcttcata caggcccgca tcttcaccag ggtggcctgc cgccgccatc cagttggcgg
    47761 aaagtttaat gcgtttgata tcgccaatct gtgttgcggc aatattggtt aacgcttcac
    47821 cgaccgccag acgggcagag gcggcgaaat ccagcagcgc aaccggcgca cgctcgccaa
    47881 tcgccatcgc ttcaccgtag tagctgtcga ggctggcggt agtgaccgcg cagttagcga
    47941 ccggcacctg ccacggcccc accatctgat cgcgcgccac catgccggtt acgctgcggt
    48001 caccgatggt caccaggaaa gttttctccg ccacggtcgg cagatgcagt acgcgtttca
    48061 ccgcgtctgc aatggtgatc ccttcacgga ccagcgcatc gcccttcgct ttcagcgttt
    48121 gtacatcgcg ggtcatcttc ggcgttttac caagcaggac gtccagcggc agatcgatcg
    48181 gctgattatc aaaatgacga tcgtgcagag aaagatgcag ttcttcggtc gcttcaccaa
    48241 tcaccgcgta gggtgcgcgc tcacgcttac acagttcgtc aaacagcggt aattgatcgg
    48301 cagcaaccgc cagcacgtag cgttcctggg attcgttaca ccagatttcc agcgggctca
    48361 tgcccggttc gtcgcttaga atatcgcgca gttcaaattt accgccgcgc ccgccgtcgc
    48421 tcaccagttc cggcatcgcg ttagaaagac cgccagcgcc gacgtcgtgg ataaacagga
    48481 ttgggttggc atcaccaagc tgccagcaac ggtcgatcac ttcctggcag cgacgctcca
    48541 tctccgggtt gtcgcgctgt acggaagcaa agtcgaggtc ggcatcagac tgacctgacg
    48601 ccatggaaga cgccgctccg ccgccaagac cgatgttcat tgccgggccg ccgagaacga
    48661 ccagtttcgc accgacgttg atctcgcctt tttgtacgtg atcggcgcga atgttgccga
    48721 tcccgcccgc cagcatgatc ggtttgtgat aaccgcgcag ctcttcgccg ttgtggctgt
    48781 tcactttttc ttcataagta cggaagtagc cattcagtgc cggacgacca aattcgttgt
    48841 taaacgccgc gccgcccagc gggccttcgg tcatgatgtc cagcgcggtg acaatgcgct
    48901 caggcttacc gaaatcttct tcccacggct gttcgaagcc aggaattcgc aggttggaaa
    48961 cggagaaacc aaccaaaccc gctttcggct tcgcgccgcg cccggtggca ccttcatcgc
    49021 ggatttcacc gccggaaccg gtcgccgcgc ccggccacgg agaaatcgcc gtcgggtggt
    49081 tgtgagtttc gactttcatc agaatatgcg ccggttcctg atggaaatcg tagcggcccg
    49141 tttcgtggtc agcaaagtag cggcccactt cagaaccttc cattacggcg gcgttatctt
    49201 tataagcaga gagaacgtga tctggcgtgg tttcgaaagt atttttgatc atcttgaaca
    49261 gcgatttcgg ctgctgttca ccgtcgataa tccagtcggc attaaaaatt ttgtggcggc
    49321 agtgctcaga gttcgcctga gcgaacatat acagctcgat gtcgttcggg ttacgaccaa
    49381 gctttgtgaa agcatcctgc agatagtcaa tttcatcttc cgccagagcc aggccaagac
    49441 gcaggttagc gtcgatcagc gcctgacggc cctgccccag caaatcaacg ctggtaaccg
    49501 gagtcggttg atggtgggca aacaactgct ctgcatcatc taaagcaaaa aagaccgttt
    49561 ccatcatgcg gtcgtgcagt tcagcggtaa cctgctgcca ttgttcattg gtcagcgtac
    49621 cggcttctat atagtaagca acgccgcgct caaggcggtt tacctgttgt agcccgcagt
    49681 tatgagcaat atcggtcgct ttcgaagacc agggagagat ggtgccagga cgcggggtca
    49741 ccagcaggag tttgccttgc ggggcgtggc tggcgagtgc cgggccatat ttcagcaggc
    49801 gttcaagttg tgcgtgctca tcatcgttta acggcgcatt gaggtcagca aaatggacat
    49861 actcggcgta aatattgtga accgggagcc tggcagcctg aaaacgtgcc agcagtttgt
    49921 tgattcggaa tgccgacagt gcaggcgaac cacgcagaat ttccatcata agtctctcgt
    49981 cttctaagct ttcggtgtac ccaagggggg aaacgggcgt cattataaag aatctgatgc
    50041 gctgacgaaa ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc
    50101 caatggctgt aataagttgc catctggcgc aggtttacgc aaaatgccgc tcatttatga
    50161 gtaaaccttt cactattatt acattttttc aagctgggac gcgcacgaca cagagaatta
    50221 actaattgaa aaaattaaag attaattatc tgttcatcgg cattctggca ctgctgctcg
    50281 cggtcgctct ctggccatcc attccctggt ttggtaaagc cgacaaccgt atcgccgcca
    50341 ttcaagcgcg gggagagttg cgtgtgagca ccattcatac tcccctgacg tataacgaaa
    50401 tcaacgggaa accttttggc ctggattacg aactggcgaa acagtttgcc gattacctcg
    50461 gcgtaaaact gaaagtgacc gtgcggcaga atatcagcca gctgtttgac gacctcgata
    50521 atggtaacgc cgacctgctg gcggcaggac ttgtctataa cagtgagcgg gtaaaaaatt
    50581 atcagcctgg ccctacctat tattccgtgt cacaacaact ggtttataaa gtgggtcagt
    50641 atcgcccacg tacgctgggc aacctgacgg cggagcaact caccgttgca ccgggtcatg
    50701 tggtggttaa cgatctccag accctgaaag aaacaaaatt cccggaatta agctggaagg
    50761 tagacgacaa aaaaggctct gcggaattaa tggaagatgt catcgaagga aaactcgatt
    50821 acaccattgc tgattctgtc gccatcagcc tgtttcagcg cgttcacccg gagctcgccg
    50881 tagcgctcga tatcaccgat gaacaaccgg tgacctggtt tagcccgtta gatggcgata
    50941 ataccctttc cgccgccctg ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg
    51001 cacgcattga agagaaatac ctggggcatg gcgatgattt tgattacgtc gatacgcgca
    51061 catttttacg cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt gagaaatacg
    51121 ccgaagaaat tgactggcgt ttgctggccg ctattgctta tcaggaatcg cactgggatg
    51181 cacaggccac ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc
    51241 aaagcctcgg cattacggat cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt
    51301 atttgcagga tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgag cggatctggt
    51361 ttgccctcgc tgcgtacaat atgggctatg cgcatatgca ggatgcccgc gccctgacgg
    51421 caaaaaccaa agggaatcct gacagttggg ctgacgtaaa acagcgtctg cctttactta
    51481 gccagaaacc ctattacagc aagctgactt acggctacgc tcgtgggcat gaagcctacg
    51541 cttatgtcga aaatattcgt aagtatcaga ttagcctggt gggttatctg caagagaaag
    51601 agaagcaggc tacagaagcg gcgatgcaac tggcgcagga ttatccggcg gtatcgccta
    51661 cggagttggg caaagagaaa tttccttttc tctcgtttct ttcccagtcg tcatcaaact
    51721 atttgaccca ttctccctct ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt
    51781 aatccgtcga ggattgcgct tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga
    51841 agtcactgag caacgccgcg cactcatccg ccagtattcc ttccgtaatt tccactcggt
    51901 gattcatacc cggatgatgc agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt
    51961 cacgcgcacc aaagaccacg cgaccaatgc gactgtggat catcgctccg gcacacatta
    52021 cacatggttc aagcgtgaca tacaacgtgg cgtcgatcag acgataattt tgcatcacca
    52081 gaccgccctg cctcagggcc atgatttctg catgtgcggt gggatcatgt cgaccaatcg
    52141 gacggttcca gccttcgcca ataacacgat tgttatgcac taataccgcg ccgaccggta
    52201 cttcccgctc atcccaggca cgcttcgcca gcgtcattgc atgacgcatc cagtattcgt
    52261 ggctaaattc gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac
    52321 acggacgcta tgctttactc tattccagtt gctggagttc accgcgcggg gttactcgcc
    52381 agcgatgctg gcaaaagtaa agcagcgggt tgtcctgatt actgtcgcta tagccactgt
    52441 acagccgcag cggcgtgccg attttgcgct ccagttgcgc gaccttttca tgcccgaggc
    52501 aacgcatcgt caatacccaa ccaccatagc cacgctgaat ttggctggcg ataagattca
    52561 cccgcggcag ccagggtgta tcgaaataga ccgcttcaac cagcggctgt ggagaaccgg
    52621 taatcaacca gatatcagca tcggaactta acaggtaggt ggttaatcgc tcctgaacca
    52681 gcggaaaggc ggtaacattg tcacgaaacc agcgtacgaa atcggcctgc aacgcctgta
    52741 aacgtgcttc gctgtgacca aaggtgcatc cccacagaag cagactcatc ggccagcgtg
    52801 ccgcacgacc ttttatcaat aacgcaatgg ctataatcgg caacaacggc aggacaagta
    52861 acgcattcag cggttggcga cgcagtaaat agcgcagaaa actgccgaac atatcctgct
    52921 gatgcaatgt tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat
    52981 actcctctgg actgaaacat cctgattaat tttcgctact acatagccta acagatagat
    53041 catcactttt ccggcaacaa tcgaccgcat catggctaac tggaattttt aattcatggc
    53101 aattagcggc aatggaatat aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc
    53161 actatttcat cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag cgttaccagg
    53221 ggcttgccca gagtgataaa aaactggcgg attatctgct gctacaacct gatacggcgc
    53281 gccatttaag ctctcagcaa ctggccaacg aagccggagt cagtcagtcc agcgtcgtga
    53341 agttcgcgca aaaactcggc tataaaggtt ttccggcgct taagttggcg ttgagtgaag
    53401 cgctggcaag ccagccggaa tcgccctcag tgcccattca taaccaaatc cgcggtgatg
    53461 atccgttacg gctggtcggc gaaaaactga ttaaagaaaa tactgccgct atgtacgcaa
    53521 cgctaaacgt taatagtgaa gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc
    53581 ggcggataat tctgaccggt attggcgctt cgggtctggt ggcgcaaaac tttgcctgga
    53641 agctgatgaa gattggcttc aatgctgccg cagtgcgcga tatgcatgcg ctactcgcta
    53701 ccgtacaggc gtcgtcccct gacgatctgt tattagccat ttcttacacc ggtgtacgac
    53761 gcgagttaaa cctggcggca gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta
    53821 ccggctttac tcccaatgcc ctgcaacagc gtgcttctca ttgcctgtat accattgccg
    53881 aagaacaagc gacaaacagt gcttcaatct ctgcttgtca cgcgcaggga atgttaacgg
    53941 atttgctgtt cattgcgctg attcagcagg atctggaact ggcaccagaa cgtattcgtc
    54001 atagtgaagc gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata atgcccgccc
    54061 ggtttgtgtt gttttgagag tttccttatg gcgctgttaa tcactaaaaa atgcatcaat
    54121 tgtgatatgt gtgaacccga atgcccgaat gaggcgattt caatgggaga tcatatctat
    54181 gagattaaca gcgataagtg taccgaatgc gtagggcact acgagacacc aacctgccag
    54241 aaggtgtgcc cgatccccaa tactattgtg aaagatccgc aacatgtcga gacagaagaa
    54301 cagttgtggg ataaatttgt gctgatgcac cacgcggata aaatttaata ttctactctg
    54361 gaagtagagt attaattatt ttactgggaa gccttaacgc cattatattt atttaattga
    54421 tgacattagc ataataattc actaagttaa tttatatagt atctgcccag acacttattt
    54481 atagttatta aaggcgcgtc cgattggttc accggacgca ccttaagtac gtttccttgt
    54541 gttataagaa cagaaggatc agctgtaaaa cagcaatgat gattttgatg acccgtttaa
    54601 tcaggtatcg gcaatcagtc attcgttttt ccttaaacaa ggaatgcagc cgttcagatt
    54661 agcccttaca tctccccaaa actgaacgtg cgagttattg gaatcgagtg ctgcactcca
    54721 caccagagct ttgacgacac cactcgtttc aatgggggaa ttcagtggca tggtgtaaag
    54781 cacagcaaaa ttgccaataa cgaaaccgat tttaatgtac ttatcaaaaa gcggtcacgt
    54841 taatcttttc atcaatgtcg atttcttcgt aaaggctcgc gttttttatg tcagagattg
    54901 caagttgctt gtacaggtca acgaaattga tccataatca ttttcgttgg aatcgtgtgc
    54961 tgcacaaaat taaagttaaa aagtaaaacc cccgttcctt accagttcgg gggttttact
    55021 ttataaagag aacggtatta tttttaactt tcaataatta ccgtggcaca agcatagtgg
    55081 cgctcatcgg ccagcgtcac atgcatattt gcaacgccca gcttttccgc cagttttaat
    55141 gcctcgcccc acagtcgcag ccgtggctta ccaagctcat cgttaaatac ttcaaactga
    55201 ttaaacgcca gaccattgcg gatcccggtg ccaaacgctt ttgctgcggc ttcttttacg
    55261 gcaaagcgtt tcgccagaaa acgcaccggc tggtggtgcg ttttccaaat ttcccattcg
    55321 ttatcgctta atacgcggcg tgccaggcgc tcaccggatc gggcgatcac tgcttctatg
    55381 cgagcgatct ctacgatatc cgtgcctaaa cctaatattg ccattagcca cgcgcttcca
    55441 gcatcaggcg cttcatttct gccaccgcat ctttcagtcc ggtcatcact gcacgaccaa
    55501 taatggcatg accgatattc agttcgtgca tctcagggat ggcggcaatg gcttttacgt
    55561 tgtgataggt cagaccgtgt ccggcgttaa ctttcaaacc gagactagcg gcaaaggtcg
    55621 cggctttggc gatacgcgcc agctcttgcg cctgttcggc gtcagttttg gcatcagcat
    55681 agcaaccggt gtggatctcg ataaacggtg cgccaacctc tgccgcagct ttgatctgct
    55741 cttcatcggc gtcgataaac agagaaacct gaatcccggc atccgccaga cgtttgcagg
    55801 catcgcgcat tttgtcacgc tgccctgcaa cgtccaggcc gccttcggtt gttacttcct
    55861 gacgcttttc tggtaccagg cagcaaaaat gcggcttcgt ctcaaccgcg atcgccagca
    55921 tctcttcggt caccgccatc tccagattca tgcgggtatc cagcgtctga cgcaggatgc
    55981 gcacatcgcg gtcagtaatg tggcggcgat cttcacgtaa atgcacggta atgccgtccg
    56041 cccccgcctg ctcggcaata aacgcggcct gcaccggatc cgggtaagcg gtaccgcgcg
    56101 cgttgcgcag cgtagcgata tggtcaatgt tgacgcctaa cagtaattca gccatgacaa
    56161 tcctcatcat tcataatgtg ttttcaccgt tcgcttaggc ataaattgcc ggaacagttc
    56221 cctgctcttt aaaggtttac cgccaagata cggcttaagc gccatgcggg taaagcgttt
    56281 cgcggcgcgc agtgtgtctg cgtcaggaaa ttcccgtgcg tttaacgctt ttaactgcct
    56341 tccggtgaac gttttattgt cgataacgac gcttgcgata aacccttttt cttcgcgata
    56401 acgatacgtc atggtgtcat ctaccggctc gccgctaccc gcacaatggg taaaattgac
    56461 gccataaccc agatgcccga gcagtgccag ttcaaagcgg cgcagcgcgg gttctggcgt
    56521 accagtgacc cctgcaagag actgaatgca gtgcaagtaa tcgaaaaaga gttcagagaa
    56581 gcgcgtctcg tattccagta cgcgggagag aagttcgttg atgtacagac cgctgtaaag
    56641 cgtgataccg cttaatggca gcgccagcga gacagcttca gcactgcgca gcgttttgac
    56701 ttcgccacgc ccgccaaaac gtagcaagag aggggtgaaa ggctgtaatg cacctttcag
    56761 ggtagagcgt ttagagcgtg cgcctttggc aaccagacgc acgcgacccg attcctccgt
    56821 gaagacgtcc agcatcaggc tggtttcgct ccacgggcga ctatgcagga caaatgcgcg
    56881 ctgccagcct tccatcggag ttactcttaa agatcgtcaa cgtaaccgag actgcgcagt
    56941 gcgcgttcgt cgtcggccca accggatttc acttttaccc acagctcaag gtgaacaggc
    57001 gcttcaaaca tttcctgcat gtctttacgc gcttcaatcc cgatggtttt gatcttggcc
    57061 cctttgttgc caatgaccat cttcttctgc ccttcacgct caacgagaat caaaccgttg
    57121 atgtcataac caccgcgttc gttagagacg aaacgttcga tctccacggt cacggagtac
    57181 ggcagttcag cgccgaggaa acgcatcagt ttttcgcgga tgatttcaga cgccataaaa
    57241 cgctgtgagc gatcggtgat gtaatcttcc gggaagtgat gagtcgcttc aggtagatgc
    57301 ttacgcacga ttgccgcaat agtggcaaca ttcagcccgg tttcggcaga gattggcacg
    57361 atatcgagga agttcatctg gcttgccagg aactgcaggt gcggcagcag atcggctttc
    57421 tcctgcacgt tgtccacttt gttcaccgcg aggattaccg gcgctttgcc ttcgcgcagt
    57481 ttgttgagca ccatttcgtc gtccggcgtc cagcgggtgc cttcaacgac aaaaatcacc
    57541 agctcaacat cgccaataga gctgctcgcc gctttgttca tcaggcggtt aatagcgcgt
    57601 ttttcttcca tatgcaggcc cggtgtatcg acgtagatcg cctgatacgc gccttcagta
    57661 tggatcccca caatgcggtg acgagttgtc tgcgccttgc gggaggtgat ggagattttc
    57721 tgccccagca gtttgttcaa caatgtggat ttgccaacat tcggacgtcc gacgatggca
    57781 ataaatccgc agtaactttt atcgatgctc attccagctc cagttttttc aacgcctgtt
    57841 cggcggcagc ctgctcagcc ttacgacggc ttgaacctgt gccaaccacc ggttcactca
    57901 ggccgctgac ctggcagtgg attgtaaatt cctgatcgtg cgcttcgcca cggacctgaa
    57961 ctaccagata agtcggcagc ggcagatgac gaccctgcaa atattcttgc aagcgcgttt
    58021 tcggatcttt ttgtttatcg cctgggctaa tttcgtccag acgagtttga taccagttga
    58081 ggattaattt ctcgacggtt tgaatatcac tgtcgaggaa tacgccacca attaatgctt
    58141 cgacggtgtc ggcgagaatt gactcacgac gaaatccacc gcttttaagt tcacctggtc
    58201 ctaaacgtaa gcactcgcct aactcaaatt cgcgcgccag ttccgccagc gtattgccac
    58261 ggaccaacgt ggcgcgcatc cggctcatat cgccttcatc cacacgaggg aaacggtgat
    58321 aaagcgcatt ggcgataacg tagctcagaa tagagtcgcc taaaaattct aaacgctcgt
    58381 tatgtttgct gctggcacta cgatgagtta atgcctgctg caacagttcc tgatgattaa
    58441 aagtgtagcc cagcttccgt tgaagccgat taattacgat ggggttcatg cgataccaat
    58501 aaataaatgc gtcaacaatt cagcacacga aacagacctg atatacatgg ctctgctaac
    58561 tgcttcgctg cagtttcagt ttagcggtat atggggacca acgctgtttc gtgtgccgtg
    58621 gcaacctgga ggtgccaacc ttaaacttcg ggggaatatt ctatacacaa cgacggggga
    58681 tgtcgttagc cacgggagat ttatctcata aataattcac gttgtcgcca taacggcgac
    58741 aacgtgaacg aagatgagct attaatggat gccgccaatg cgacttaagc gcacacctgt
    58801 cggccattcg ccttcttgct tatcgaagct catccagata gccgttgccc gaccgaccag
    58861 attcgcttca ggcacaaagc cccagtaacg gctgtccgcg ctgttgtcgc ggttgtcgcc
    58921 catcatgaag tattgtcccg gaggaacaat ccaggttgcc agttgttgcc ctggctgctg
    58981 gtaatacatc cccacctgat cctgcgcaat cggcactgtc agaatgcggt gcgtcacatc
    59041 acccagtgtc tctttacgct cggaaagacg aattccattt tctttggttt cgtttttcgg
    59101 cacttcaaag aatccgctgg tcgcttcccc accattacgg cgtgagaagg tctgaacgaa
    59161 atcgctcggt tccacgtttg agtaggtgac cggcagcgcg ttttcacacg cctggccgga
    59221 actgcatccc ggttgaatcg tcagctcttt tgagaccgga tcgtaagtga ctttatcgcc
    59281 cggtaaaccc accgcgcgct tgatataatc aagctttgga tcttccggat atttaaagac
    59341 cacgatatcg ccgcgtttcg gatgaccggt ttcgatcagc gttttctggt agataggatc
    59401 tttaatgcca taagcaaact tctctaccag aataaaatca ccgattaaca gagtcggcat
    59461 catcgaacct gacggaatct ggaacggttc ataaataaac gaacgcacaa tcaatacgat
    59521 agccagtacc ggaaaaacag aagcaccggt ttccagccag ccaggcttcg gcgcaacctt
    59581 tttcaacgtt gctttatcca gtgagtcacc ggcagccgcc tgcgccgctg cctgacgttc
    59641 ccgccgttta ggtgcgaaaa agaatttatc cacgcaccat aaaatgcccg tcaccagtgt
    59701 ggcaatcacc agaatcaggg caaacatatt cgccatgcca actcctaagg gttatttgtt
    59761 gtctttgccg acgtgcagaa tggcgaggaa cgcttcctgc ggcagctcga cgttaccgat
    59821 ctgcttcatg cgtttcttac cttctttctg cttctgcagc agctttttct tacggctgat
    59881 atcgccgcca taacatttag ccagtacgtt tttacgcagc tgtttcacgg tggatcgcgc
    59941 aataatgtgc gtaccaatcg ctgcctgaat ggcgatatca aactgctggc gtgggatcag
    60001 atctttcatc ttctccacca actcgcgacc gcggttttgc gaattatcac ggtgggtgat
    60061 caacgccagc gcatcaacac gttcaccgtt gattaatacg tctacacgta ccatgtcgga
    60121 cgcctggaag cgtttgaagt tgtaatccag agacgcataa ccacgcgagg tagatttcag
    60181 gcgatcgaag aagtcgagca ccacttccgc catcgggatc tcgtacgtca gcgccacctg
    60241 attaccgtgg taaaccatat tggtctgcac gccgcgtttt tctacgcaca gcgtaataac
    60301 gttgccgaga tatgcctgcg gcagcagcat gtgacactct gcaatcggct cgcgcagttc
    60361 gtaaatgtta tttaccgcag gcagcttgga tgggctgtcg acgtagataa cttcccttga
    60421 cgtggtttca acttcataca ctacggtcgg cgcagtggtg atcagatcca gatcgtattc
    60481 acgttccaga cgctcctgga tgatctccat gtgcagcagg ccaaggaagc cgcagcggaa
    60541 accaaagccc agcgcgctgg agctttccgg ctcatagaac agtgaggcat cgttcaggct
    60601 gagtttaccc agcgcgtcac ggaaggcttc atagtcgtcg gaacttaccg ggaacagacc
    60661 ggcgtatacc tgcggtttga ctttcttaaa gccaggcagc gccttttctg ccggattacg
    60721 cgccagcgtt aaggtatcgc cgactggagc gccgtggata tctttaatcg cacatacgag
    60781 ccagcctact tcgccacatt tcagttcagt gcggtcaacc tgtttcggcg tgaagatgcc
    60841 cagacggtcg gcgttatagg tctgcccggt actcatgact ttcactttgt cgcccttacg
    60901 cagggtgccg tttttaatac ggataagtga aacaacgccc aggtagttgt cgaaccatga
    60961 gtcgataatt agtgcctgca acgggccttc cggatcgcct tccggcggcg gaatgtcgcg
    61021 caccagacgt tcgagaacgt cctgcacgcc aacgccggtt ttcgctgaac agcgcaccgc
    61081 gtcagtggcg tcgatgccga cgatatcttc aatttcttcc gccacgcgtt caggatcggc
    61141 tgccggcagg tcaatcttgt tcagtaccgg caccacttcg agatccattt ccatggcggt
    61201 gtagcagttt gccagggttt gcgcttccac gccctgcccg gcgtcgacca ccagcaatgc
    61261 accttcacag gcagccagcg aacgggaaac ttcataggag aagtctacgt ggcccggggt
    61321 gtcgataaag ttaagctgat aggtttcgcc gtcagacgct ttgtagtcca gcgtcacgct
    61381 ttgcgctttg atggtaatgc cacgctcacg ctcaagatcc atggaatcga gaacctgcgc
    61441 ctccatttca cggtcagaca ggccaccgca gatctggata atacggtcag acagcgtcga
    61501 tttaccgtgg tcaatgtgag ctatgatcga aaagttacgt atattcttca taaagtatga
    61561 ttattgtgcc ttaatgcccg gttaaccagg cttttagaag tcgctgttct gagcttaacg
    61621 tctgtattaa tagaaacgcc gcattctaca ctacaacatt gaggcgagga aatgttcata
    61681 ccgtatggat tgtggtatct ggaaacgtcc tcgcatttgt tatgcaaaat gcaacaaagc
    61741 cagtgaaatc actggctcgc gtcttccgaa gatgtttcaa atcgcacaag gccaggcggc
    61801 aatgccacgc ttaagatgat cggttgccat tctgcccggg cagcaaactt acgcgagtag
    61861 ccacgcgcaa tcaggaaccc gccaataccg ccgaggaccg caccacataa tgctgcgacg
    61921 tcagaagcaa agagtagctg aaatagcgaa gcgatgagga ataatcccac cagcggcgac
    61981 atataaacca gtaatgcgga gctaagcagg ctgccttcgg cgatccccaa ttccactttt
    62041 tgccccggca ctaacggctc atcacagggt acgacaatgg tatgcgtggt ttgcgggcca
    62101 agtttattca acacgcggct accgcaaccg gcgcgtgaag cgcagctgct gcatgaggct
    62161 ttaacatcac aactgaccag cgcctgcccg ttttgccagg agacgacggt agcccactct
    62221 ttgatcattg cgctgccccg aacttaatat tctcggcaat gcgtttcgcc gtttgcggcg
    62281 gcagttcacc gacaatggtg atttcggcgt tatcacgtac gcttgtactg acggttctgc
    62341 gtccggtgcg caacatctga tcggtgctcg atggcgtagc gcggttaacg tttaccgaga
    62401 agctgaataa tccgtcggaa tagagacgtg attcgatagg catgttgtcc atcgtcggta
    62461 gcggacgtcg actactggaa acttcgctaa aaccctgtgg caaccaggtt ggcgtccagc
    62521 tgaatttagc tttttcacct acaggaacag aaagcaacgg cggcaaattt gccttcgcca
    62581 gcgtctgcat actgctgctg atatcctgat tgacgttaaa agcaatcacg cgaaattgtt
    62641 ccagcgtttc accatcgcga tcaagaagat caacccgcat cggtaatttc gattcggtgt
    62701 ccatccacac gatgtagctg tagcgtgtac catctcgggc aaccacgcga atgacttcgc
    62761 aaagacgatc agcaatacgc gtgcgcccga cggagataaa gtcgtagtaa ggagaaaggc
    62821 gtttgaaatc ggtatagatg agcgatggca gagaatcaac aatgtaatcg ccattaagcg
    62881 tgaacggttc aagtcccggt tcaaaatagc tgatttcatt gccgcgctgt accacttccc
    62941 ggcgcgggcc atccatttgc aacaattgtg caagaggacg gttatcgagg cgtgcatgtc
    63001 gataacgcag agactcaaca ccctgtttat tgatgctgat gaatgacagc tcgtaattca
    63061 gtgactgact ggccaggttc atctgctgta ataacgcccc ggacgcggga gtggccgagg
    63121 cgttagcaga gaataacagg ctacctgtca ctaatgacat ggcaaaccaa agttgcttca
    63181 ttactgcgat tgcgttccta aagtttgaat tcctggcacc tgtacagcgg cttgctgggt
    63241 ttgcgcctgc tcaaactgaa gctgttcaga gtggagtcgg cgttgcagtt cgtaatcctg
    63301 caacattgca ttaatgcgac gacgctgctg ctgttgacca ttatttgcgg tcgcttcaga
    63361 aggtactccc aggctaaccg ggctggcttt acccatcatc ggcagtgtat taaataccgg
    63421 cgtttcgggc tgctgggacg tttcagattg tccattatag tgctggacgc caacgataac
    63481 tgcaagcgat acgcatgcgg ctacgcccat ttgggtaagc tgtgccgccc acggacgtac
    63541 tttctgccag aatggcattt tctgccattc atgcggcgca ggctgggctt ccgggatcaa
    63601 tgtcgccggt tgacgtactg gctcttcttc aatggcggcc atcacgcgtg aagagatatc
    63661 gaaatggagc acctcgggag tatcaccccg cattgagtca cggattaagt gatagctttc
    63721 ccaggttttc tgcatttctg ggttatgagc cagttcgtta agcagctcac tatccagcgt
    63781 ttcgccatcc attaaagcgg aaagttgttc tttctgcatg cctaataccc ttatccagta
    63841 tcccgctatc gtcaacgcct gataagcggt tgaactttgt tatcaatagc ttccctcgct
    63901 cggaagatac gtgaacgcac cgtacctacc ggacaatcca tgatagcggc tatctcttca
    63961 tagctcaggc catccagctc ccgcaaggtt attgccatgc gtaaatcttc cgggagggac
    64021 tcaatagttc ggaaaactat ctgtctcagt tcttctgaca acattaagtt ctcagggttc
    64081 gaaatttctt tcaacgcgcc gccactttcg aagttttcag cttcaatggc atccacatca
    64141 ctggaaggtg gacgacgccc ctgagcaacc aggtaatttt tcgctgtatt tacagcaatc
    64201 cgatacagcc atgtataaaa agcgctatct ccccggaacg aatccagcgc acgataggct
    64261 ttaataaaag cttcttgtac cacatcggga acatcacccg acggcacata gcgggaaacc
    64321 agactcgcca ctttatgctg atagcgcact accagtaagt taaaggcttt ctgatctccc
    64381 ttctggaccc gttcaaccag gacctggtcc gttaactgct cgctcatccg aggtaaagtc
    64441 tccccaaacc aaatttccac gcgctatcga aacgccactc cattagctgc aatttgagca
    64501 agcaaagggt tagagtgtct cgtttttgta aagttccgta acgcatctgt ttttgtttgt
    64561 catgctgtag acggatcatt atctatcatt ataagtctac agaatctgaa catcgcatta
    64621 tctgtgtaga aatgcccatt taactgcctg aagagtaacc caacggcctt tttatttcac
    64681 cacctaatcc tccaccagcc agtaacttct ctttttctcg ccgccctgcg tcagcgtgtt
    64741 tagtcactgt aacaaatatt aaaatagcag gtgtttattc gcacaacatg atgctatgct
    64801 gaccaaaccg tgtttagtaa attaaacaaa gaaaatgaat actctccttg aacattcatg
    64861 tgacgtgttg attatcggta gcggcgcagc cgggctttca ctggcgctac gcctggctga
    64921 ccagcatcag gtcatcgttc taagtaaagg cccggtaacg gaaggttcaa cattttatgc
    64981 ccagggcggt attgccgccg tgtttgatga aactgacagc attgactcgc atgtggaaga
    65041 cacattgatt gccggggctg gtatttgcga tcgccatgca gttgaatttg tcgccagcaa
    65101 tgcacgatcc tgtgtgcaat ggctaatcga ccagggggtg ttatttgata cccacattca
    65161 gccgaatggc gaagaaagct accatctgac ccgtgaaggt ggacatagtc accgtcgtat
    65221 tcttcatgcc gccgacgcaa ctggtagaga agtagaaacc acgctggtga gcaaggcgct
    65281 gaaccatccg aatattcgcg tgctggagcg cagcaacgcg gttgatctga ttatttctga
    65341 caaaattggc ctgccgggca cgcgacgggt tgttggcgcg tgggtatgga accgaaataa
    65401 agaaaaggtg gaaacctgcc acgcaaaagc ggtggtgctg gcaaccggcg gtgcgtcgaa
    65461 ggtttatcag tacaccacca atccggatat ttcttctggc gatggcattg ctatggcgtg
    65521 gcgcgcaggc tgccgggttg ccaatctcga atttaatcag ttccacccta ccgcgctgta
    65581 tcacccacag gcacgcaatt tcctgttaac ggaagcactg cgcggcgaag gcgcttatct
    65641 caagcgcccg gatggcacgc gttttatgcc cgattttgat gtgcgcggcg aactggcccc
    65701 gcgcgatatt gtcgcccgcg ccattgacca tgaaatgaaa cgcctcggcg cagattgtat
    65761 gttccttgat atcagccata agcccgccga ttttattcgc cagcattttc cgatgattta
    65821 tgaaaagctg ctcgggctgg ggattgatct cacacaagaa ccggtaccga ttgtgcctgc
    65881 tgcacattat acctgcggtg gtgtaatggt tgatgatcat gggcgtacgg acgtcgaggg
    65941 cttgtatgcc attggcgagg tgagttatac cggcttacac ggcgctaacc gcatggcctc
    66001 gaattcattg ctggagtgtc tggtctatgg ctggtcggcg gcggaagata tcaccagacg
    66061 tatgccttat gcccacgaca tcagtacgtt accgccgtgg gatgaaagcc gcgttgagaa
    66121 ccctgacgaa cgggtaataa ttcagcataa ctggcacgag ctacgtctgt ttatgtggga
    66181 ttacgttggc attgtgcgca caacgaagcg cctggaacgc gccctgcggc ggataaccat
    66241 gctccaacaa gaaatagacg aatattacgc ccatttccgc gtctcaaata atttgctgga
    66301 gctgcgtaat ctggtacagg ttgccgagtt gattgttcgc tgtgcaatga tgcgtaaaga
    66361 gagtcggggg ttgcatttca cgctggatta tccggaactg ctcacccatt ccggtccgtc
    66421 gatcctttct cccggcaatc attacataaa cagataaaaa gcctgggtta gcgccgtata
    66481 cgcttcggaa tagttctggt ctggcccacg aatgactaag cgatcgctaa agcattctcc
    66541 cgcctgcggg gagaatgcca gcagcacccg atgcggcagt cgcgcttcgt tttccgccac
    66601 atccgtccgc aaacgtaaat gccagcccat acttaatgcc agctccgtaa aactattacc
    66661 aatctgctct ggcagcacca cgcagaaaaa tccctcttcg gtaatgcact ccgccgcgca
    66721 agtaagtaaa gtctggtgat caagtgaggt ggtgtagcgc gcctgttccc gctgcggcgt
    66781 cgcgcactcc actccttgct cataatacgg cgggttgctg atgattaaat caaagcgcgc
    66841 ggtctgctgt gttacccact gctgaatatc cgccgtatgg acgttaattc tctctaacca
    66901 cggggactgg gcgacatttt cctgcgcctg agtggcagct tcactttcca gctcaacggc
    66961 atcaattatc acgctgtcac tggttcgctg cgccagcatc agtgccagca agccactacc
    67021 cgcaccgata tcaaggcaac gttttacccc agccaccggt gcccatgcgc ccagcaatat
    67081 gccatccgtt cccactttca tcgcacagcg atcgtgagca acaaaaaact gtttaaaagt
    67141 aaatccatta cgacgaagca cggatgtaga ctgtgacatg aaaataaaac cttgcaggaa
    67201 aaacggcgat agcaccgggt gagaacaata cccgagaagc gatatccata caaacagatg
    67261 aagattgcag ccgtaacgtc tataatcagc gccccacaca gaggtagaac atgactgtaa
    67321 cgactttttc cgaacttgaa ctcgacgaaa gcctgctgga agccctccag gataaaggtt
    67381 tcactcgccc gaccgccatt caggctgccg ccattccgcc tgcgctcgat ggccgtgatg
    67441 tactcggttc tgcgccgaca ggcaccggta aaacggcggc gtatctgctg ccagcgttgc
    67501 agcacctgct cgatttcccg cgtaagaaat ccggtccgcc gcgtattttg atcctcaccc
    67561 caactcgcga gctggcgatg caggtggccg atcatgcccg cgaactggcg aaacatacgc
    67621 atctggatat cgccaccatc accggcggcg tagcctatat gaaccacgcg gaagtgttca
    67681 gcgaaaatca ggacatcgtg gtcgccacga ccggacgtct gctgcaatac ataaaagaag
    67741 agaacttcga ttgccgcgcg gttgaaacgc tgatcctcga cgaagcagac cgtatgctgg
    67801 atatgggctt cgctcaggat atcgaacata ttgctggcga aacgcgctgg cgtaaacaga
    67861 ccctgctctt ttcggcaacg ctggaaggcg atgcgattca ggactttgcc gagcgtctgc
    67921 tggaagatcc ggtggaggtt tctgccaatc cgtccacccg tgagcgcaaa aaaattcatc
    67981 agtggtatta ccgcgccgat gacctggaac ataaaaccgc attgctggtg catctgttaa
    68041 aacagccgga agcgacccgc tcaattgtgt ttgtgcgtaa gcgtgagcgt gtgcatgagc
    68101 tggcaaactg gctgcgcgaa gcgggcatca acaactgcta tctcgaaggt gagatggtac
    68161 agggcaagcg taacgaagcg atcaaacgtt tgaccgaagg tcgcgtaaac gtactggttg
    68221 caaccgatgt tgccgcgcgc ggtatcgaca ttcctgacgt cagccacgtc tttaacttcg
    68281 atatgccgcg cagtggcgat acttatttgc accgtatcgg acgtaccgcg cgcgccggtc
    68341 gtaaaggcac cgcaatttcg ctggtagaag cccatgacca tctgctgctg ggcaaagttg
    68401 gtcgctatat tgaagagcca attaaagctc gcgtaattga tgagttacgc ccgaaaacgc
    68461 gtgcaccaag cgagaagcag accggcaagc catcgaagaa agtactggca aaacgcgccg
    68521 agaagaaaaa agctaaagag aaagagaagc cgcgggtgaa aaaacgccat cgcgatacca
    68581 aaaatattgg taagcgccgt aaaccaagcg gaacgggcgt gccaccgcaa acgacagaag
    68641 agtaatctca atgccgggtt taagcccggc attattttta tcccggcaaa aaacaatctt
    68701 caacacactg aataaaagtg tgcatcgccg ggctaaccgc ttttccggca tggtgagcgc
    68761 acattgccgt aatggtctgc gagtgttcgc caaaaggcaa ttcaattaat tctccgctct
    68821 caagctcttt ttccaccgca aagcggggca agtaactgac accgatattc gccgcgacac
    68881 aacgtttgat actttcaatg cttaacagct caatggtgtt ttccaccgtg atccgccgct
    68941 ggcgcagcgt gctctcaaat atctgccgga atacacattg cggttcgttg ataataaagc
    69001 tacaggcgtt atgtcttccc ggctcagtaa aatcgacatc tgcaatttgc ggtgaagcta
    69061 ccagcaccag cgattgctca cccaattctc gtcggttcag ggcatcatca ttccctacgc
    69121 gataaaaaac cccgacatcg gcctcatcat tcaatagcgc atcacggatc acgtaacagt
    69181 tcagagactg caacgataaa cgcacttttg gggcgcgttg ccgaaaacgc tgcaacacct
    69241 gcggcattcg gtaagagagc aacgtttcgc ccgaaacaac gcgtagttcc ccgtccggat
    69301 ccgactcctt tttggcagcc tcacgaaggg tatccatcac tctggtgagt tcataaatgt
    69361 gcggcagcag ctttttacct tcgcgagtga ggcacatccg tcggccaatt ttctcaaata
    69421 actgtaccga gaactcctgc tcaagctgct gaatatgaaa agtcaccgtc gattgtgtac
    69481 agcacaattt ttgcgaagct cgcaaaaagg aaccctcttc caccacggtt ttaagcgtaa
    69541 taaaacggcg cagatccata accccaaacc tatcgaaaat atcgaattta gaatgtaaaa
    69601 a
//
LOCUS       DAZKFZ010000003        67468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000003 DAZKFZ010000000
VERSION     DAZKFZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..168
                     /locus_tag="ACHOQW_000152"
     CDS             <1..168
                     /locus_tag="ACHOQW_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9571306.1"
                     /translation="YAKAGMIFPLPKKVGRRWRVDPQARFVGMVNKPEVIATDHPALK
                     RILEDGAPAKI"
     gene            146..1276
                     /locus_tag="ACHOQW_000153"
     CDS             146..1276
                     /locus_tag="ACHOQW_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9571307.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1322..1960)
                     /gene="ompW"
                     /locus_tag="ACHOQW_000154"
     CDS             complement(1322..1960)
                     /gene="ompW"
                     /locus_tag="ACHOQW_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP9571308.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2200..2289
                     /locus_tag="ACHOQW_000155"
                     /pseudo
     CDS             2200..2289
                     /locus_tag="ACHOQW_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2317..3060
                     /gene="yciC"
                     /locus_tag="ACHOQW_000156"
     CDS             2317..3060
                     /gene="yciC"
                     /locus_tag="ACHOQW_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP9571309.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3090..3629
                     /gene="yciB"
                     /locus_tag="ACHOQW_000157"
     CDS             3090..3629
                     /gene="yciB"
                     /locus_tag="ACHOQW_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP9571310.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3734..4132
                     /gene="yciA"
                     /locus_tag="ACHOQW_000158"
     CDS             3734..4132
                     /gene="yciA"
                     /locus_tag="ACHOQW_000158"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP9571311.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(4172..4891)
                     /gene="tonB"
                     /locus_tag="ACHOQW_000159"
     CDS             complement(4172..4891)
                     /gene="tonB"
                     /locus_tag="ACHOQW_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP9571312.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5115..5411
                     /gene="yciI"
                     /locus_tag="ACHOQW_000160"
     CDS             5115..5411
                     /gene="yciI"
                     /locus_tag="ACHOQW_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP9571313.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5712..6965
                     /gene="kch"
                     /locus_tag="ACHOQW_000161"
     CDS             5712..6965
                     /gene="kch"
                     /locus_tag="ACHOQW_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP9571314.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7020..7193)
                     /gene="yciY"
                     /locus_tag="ACHOQW_000162"
     CDS             complement(7020..7193)
                     /gene="yciY"
                     /locus_tag="ACHOQW_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP9571315.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7336..8796
                     /gene="cls"
                     /locus_tag="ACHOQW_000163"
     CDS             7336..8796
                     /gene="cls"
                     /locus_tag="ACHOQW_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9571316.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8831..9160
                     /gene="yciU"
                     /locus_tag="ACHOQW_000164"
     CDS             8831..9160
                     /gene="yciU"
                     /locus_tag="ACHOQW_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP9571317.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9213..10217)
                     /gene="oppF"
                     /locus_tag="ACHOQW_000165"
     CDS             complement(9213..10217)
                     /gene="oppF"
                     /locus_tag="ACHOQW_000165"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP9571318.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10214..11227)
                     /gene="oppD"
                     /locus_tag="ACHOQW_000166"
     CDS             complement(10214..11227)
                     /gene="oppD"
                     /locus_tag="ACHOQW_000166"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP9571319.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11239..12147)
                     /gene="oppC"
                     /locus_tag="ACHOQW_000167"
     CDS             complement(11239..12147)
                     /gene="oppC"
                     /locus_tag="ACHOQW_000167"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP9571320.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12162..13082)
                     /gene="oppB"
                     /locus_tag="ACHOQW_000168"
     CDS             complement(12162..13082)
                     /gene="oppB"
                     /locus_tag="ACHOQW_000168"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP9571321.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13168..14799)
                     /gene="oppA"
                     /locus_tag="ACHOQW_000169"
     CDS             complement(13168..14799)
                     /gene="oppA"
                     /locus_tag="ACHOQW_000169"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP9571322.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(15537..16184)
                     /gene="ychE"
                     /locus_tag="ACHOQW_000170"
     CDS             complement(15537..16184)
                     /gene="ychE"
                     /locus_tag="ACHOQW_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP9571323.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(16215..16268)
                     /gene="ychE"
                     /locus_tag="ACHOQW_000171"
     CDS             complement(16215..16268)
                     /gene="ychE"
                     /locus_tag="ACHOQW_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP9571324.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16661..19336
                     /gene="adhE"
                     /locus_tag="ACHOQW_000172"
                     /gene_synonym="adhC"
     CDS             16661..19336
                     /gene="adhE"
                     /locus_tag="ACHOQW_000172"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9571325.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(19637..20254)
                     /gene="tdk"
                     /locus_tag="ACHOQW_000173"
     CDS             complement(19637..20254)
                     /gene="tdk"
                     /locus_tag="ACHOQW_000173"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9571326.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            20858..21271
                     /gene="hns"
                     /locus_tag="ACHOQW_000174"
     CDS             20858..21271
                     /gene="hns"
                     /locus_tag="ACHOQW_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP9571327.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(21415..22323)
                     /gene="galU"
                     /locus_tag="ACHOQW_000175"
     CDS             complement(21415..22323)
                     /gene="galU"
                     /locus_tag="ACHOQW_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9571328.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(22525..23538)
                     /gene="rssB"
                     /locus_tag="ACHOQW_000176"
     CDS             complement(22525..23538)
                     /gene="rssB"
                     /locus_tag="ACHOQW_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP9571329.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(23630..24535)
                     /gene="rssA"
                     /locus_tag="ACHOQW_000177"
     CDS             complement(23630..24535)
                     /gene="rssA"
                     /locus_tag="ACHOQW_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP9571330.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            24648..25106
                     /gene="ychJ"
                     /locus_tag="ACHOQW_000178"
     CDS             24648..25106
                     /gene="ychJ"
                     /locus_tag="ACHOQW_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP9571331.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            25156..25998
                     /gene="purU"
                     /locus_tag="ACHOQW_000179"
     CDS             25156..25998
                     /gene="purU"
                     /locus_tag="ACHOQW_000179"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP9571332.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            26158..26242
                     /locus_tag="ACHOQW_000180"
     tRNA            26158..26242
                     /locus_tag="ACHOQW_000180"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26192..26194,aa:Tyr,seq:gta)
     gene            26287..26417
                     /locus_tag="ACHOQW_000181"
     ncRNA           26287..26417
                     /ncRNA_class="other"
                     /locus_tag="ACHOQW_000181"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            26452..26536
                     /locus_tag="ACHOQW_000182"
     tRNA            26452..26536
                     /locus_tag="ACHOQW_000182"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26486..26488,aa:Tyr,seq:gta)
     gene            26583..26714
                     /locus_tag="ACHOQW_000183"
     ncRNA           26583..26714
                     /ncRNA_class="other"
                     /locus_tag="ACHOQW_000183"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            26761..26892
                     /locus_tag="ACHOQW_000184"
     ncRNA           26761..26892
                     /ncRNA_class="other"
                     /locus_tag="ACHOQW_000184"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(27076..27753)
                     /gene="narI"
                     /locus_tag="ACHOQW_000185"
     CDS             complement(27076..27753)
                     /gene="narI"
                     /locus_tag="ACHOQW_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9571333.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(27753..28463)
                     /gene="narJ"
                     /locus_tag="ACHOQW_000186"
     CDS             complement(27753..28463)
                     /gene="narJ"
                     /locus_tag="ACHOQW_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9571334.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(28460..29998)
                     /gene="narH"
                     /locus_tag="ACHOQW_000187"
     CDS             complement(28460..29998)
                     /gene="narH"
                     /locus_tag="ACHOQW_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9571335.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(29995..33738)
                     /gene="narG"
                     /locus_tag="ACHOQW_000188"
     CDS             complement(29995..33738)
                     /gene="narG"
                     /locus_tag="ACHOQW_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP9571336.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(34131..35522)
                     /gene="narK"
                     /locus_tag="ACHOQW_000189"
     CDS             complement(34131..35522)
                     /gene="narK"
                     /locus_tag="ACHOQW_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP9571337.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            35861..37657
                     /gene="narX"
                     /locus_tag="ACHOQW_000190"
     CDS             35861..37657
                     /gene="narX"
                     /locus_tag="ACHOQW_000190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP9571338.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            37650..38300
                     /gene="narL"
                     /locus_tag="ACHOQW_000191"
     CDS             37650..38300
                     /gene="narL"
                     /locus_tag="ACHOQW_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP9571339.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(38301..39695)
                     /gene="ychO"
                     /locus_tag="ACHOQW_000192"
                     /gene_synonym="iatA"
     CDS             complement(38301..39695)
                     /gene="ychO"
                     /locus_tag="ACHOQW_000192"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP9571340.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            39881..40234
                     /gene="ychN"
                     /locus_tag="ACHOQW_000193"
     CDS             39881..40234
                     /gene="ychN"
                     /locus_tag="ACHOQW_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP9571341.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(40278..40973)
                     /gene="chaC"
                     /locus_tag="ACHOQW_000194"
     CDS             complement(40278..40973)
                     /gene="chaC"
                     /locus_tag="ACHOQW_000194"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP9571342.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(41134..41364)
                     /gene="chaB"
                     /locus_tag="ACHOQW_000195"
     CDS             complement(41134..41364)
                     /gene="chaB"
                     /locus_tag="ACHOQW_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP9571343.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            41634..42734
                     /gene="chaA"
                     /locus_tag="ACHOQW_000196"
     CDS             41634..42734
                     /gene="chaA"
                     /locus_tag="ACHOQW_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP9571344.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            43133..43240
                     /locus_tag="ACHOQW_000197"
     CDS             43133..43240
                     /locus_tag="ACHOQW_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9571345.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            43669..43776
                     /locus_tag="ACHOQW_000198"
     CDS             43669..43776
                     /locus_tag="ACHOQW_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9571346.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(43924..44778)
                     /gene="kdsA"
                     /locus_tag="ACHOQW_000199"
     CDS             complement(43924..44778)
                     /gene="kdsA"
                     /locus_tag="ACHOQW_000199"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP9571347.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(44814..45623)
                     /gene="sirB1"
                     /locus_tag="ACHOQW_000200"
     CDS             complement(44814..45623)
                     /gene="sirB1"
                     /locus_tag="ACHOQW_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP9571348.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(45627..46019)
                     /gene="sirB2"
                     /locus_tag="ACHOQW_000201"
     CDS             complement(45627..46019)
                     /gene="sirB2"
                     /locus_tag="ACHOQW_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP9571349.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLTLVVLYIIIKLATTKVPLLG"
     gene            complement(46016..46849)
                     /gene="prmC"
                     /locus_tag="ACHOQW_000202"
     CDS             complement(46016..46849)
                     /gene="prmC"
                     /locus_tag="ACHOQW_000202"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9571350.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(46849..47931)
                     /gene="prfA"
                     /locus_tag="ACHOQW_000203"
     CDS             complement(46849..47931)
                     /gene="prfA"
                     /locus_tag="ACHOQW_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9571351.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(47973..49229)
                     /gene="hemA"
                     /locus_tag="ACHOQW_000204"
     CDS             complement(47973..49229)
                     /gene="hemA"
                     /locus_tag="ACHOQW_000204"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP9571352.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            49443..50066
                     /gene="lolB"
                     /locus_tag="ACHOQW_000205"
     CDS             49443..50066
                     /gene="lolB"
                     /locus_tag="ACHOQW_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP9571353.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            50066..50917
                     /gene="ispE"
                     /locus_tag="ACHOQW_000206"
     CDS             50066..50917
                     /gene="ispE"
                     /locus_tag="ACHOQW_000206"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9571354.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            51068..52015
                     /gene="prs"
                     /locus_tag="ACHOQW_000207"
     CDS             51068..52015
                     /gene="prs"
                     /locus_tag="ACHOQW_000207"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9571355.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            52140..53819
                     /gene="dauA"
                     /locus_tag="ACHOQW_000208"
     CDS             52140..53819
                     /gene="dauA"
                     /locus_tag="ACHOQW_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP9571356.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(53874..54152)
                     /gene="ychH"
                     /locus_tag="ACHOQW_000209"
     CDS             complement(53874..54152)
                     /gene="ychH"
                     /locus_tag="ACHOQW_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP9571357.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            54430..55014
                     /gene="pth"
                     /locus_tag="ACHOQW_000210"
     CDS             54430..55014
                     /gene="pth"
                     /locus_tag="ACHOQW_000210"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9571358.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            55131..56222
                     /gene="ychF"
                     /locus_tag="ACHOQW_000211"
     CDS             55131..56222
                     /gene="ychF"
                     /locus_tag="ACHOQW_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9571359.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            57066..59951
                     /gene="ycgV"
                     /locus_tag="ACHOQW_000212"
     CDS             57066..59951
                     /gene="ycgV"
                     /locus_tag="ACHOQW_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9571360.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAILFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTSYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNIYVKTGAIREFSGDTEYQLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
     gene            complement(60051..61970)
                     /gene="dhaR"
                     /locus_tag="ACHOQW_000213"
     CDS             complement(60051..61970)
                     /gene="dhaR"
                     /locus_tag="ACHOQW_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP9571361.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCVTPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            62198..63268
                     /gene="dhaK"
                     /locus_tag="ACHOQW_000214"
     CDS             62198..63268
                     /gene="dhaK"
                     /locus_tag="ACHOQW_000214"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9571362.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     GVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            63279..63911
                     /gene="dhaL"
                     /locus_tag="ACHOQW_000215"
     CDS             63279..63911
                     /gene="dhaL"
                     /locus_tag="ACHOQW_000215"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9571363.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSCVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAEFAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            63922..65340
                     /gene="dhaM"
                     /locus_tag="ACHOQW_000216"
     CDS             63922..65340
                     /gene="dhaM"
                     /locus_tag="ACHOQW_000216"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9571364.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSPL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSPVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            65660..67344
                     /gene="treA"
                     /locus_tag="ACHOQW_000217"
                     /pseudo
     CDS             65660..67344
                     /gene="treA"
                     /locus_tag="ACHOQW_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT        17081 a        17173 c        16401 g        16813 t
ORIGIN      
        1 tacgctaaag ccggaatgat atttcctctc cccaaaaaag ttggaagacg ctggcgagtg
       61 gatccgcaag ctcgctttgt cggaatggta aacaagccgg aggtgatcgc cacagatcac
      121 cctgctttga agaggatact ggaagatggc gcgcccgcga aaatataaaa ccgatgttcc
      181 gggattatct ccgtattttg acaaaagaaa taacaaagtt tactggcgtt acaggcatcc
      241 cataacaggc aaaaatcacg gtctcggcag tattgaccag aaactggcag aaactattgc
      301 agcagaagcg aacagccgtc ttgcccggca gcaaatggaa caaatgctca gtctgcagga
      361 gaaaattatt agtgataccg gcggttcatc aaccgttacc atttttctga ataattacag
      421 aaaaattcaa caggaaagat atgaaaacgg cgagatcaaa ctcaacacgc tgaaacagaa
      481 agcggcccct ctcagggtat ttgatgaacg ttttggcacc agaccgttag atgccataac
      541 cgtaaaagat gtggtatcag tactggaaga gtacaaggcc agaggacata acagaatggg
      601 acaaattttc aggaaagtac tgatcgatgt tttccgggaa gctcagcaaa cgggcgatgt
      661 cccgccaggc tttaaccctg cagaatctgc aaaaaaaccg caggtgcgga tatcaagaca
      721 gcgactgact tttgatgagt ggatgatgat ttataacgca gcggaaaagg atggttactt
      781 tttacagcgc ggtatgctgc tggcactgat gacaggccag cgcctttcag atatttgcaa
      841 aatgcaattt tcggatatcc gggatggtta tcttcatgtc gaacagcaaa aaacaggaac
      901 ccggattgcc atccctctgg ctctgcgttg cgataaatta aatctcaccc tggatgatgt
      961 ggtgtcatcc tgccgcgatt gcgttcttag tccgtggcta ttgcaccacc atcacgcgaa
     1021 agggacagct aagcgcggcg ggatggttaa gccagcaaca ttaaccgttg catttaaaaa
     1081 agcccgggat tctgtggatt acaactggcg tgctaatggc acccctccct ctttccatga
     1141 gcagagatct ttatcagagc gattgttcag agagcagggg gttgatacca aaattttgct
     1201 aggccattcg aatcaaaaaa tgaccgatat ttacaacgac gcacgcggta aggaatggaa
     1261 aaaactggtc atttgatgac cagttttgca gaggggtttt gcagaggttt tgcagagaaa
     1321 attaaaaacg atatcctgcg gagaacataa acacccacgg atccagtcgt accgagtctt
     1381 ttacggtagt aacaccagat ttgtacttag ccgtggtatc gatatccatg taccacactg
     1441 acatgttaac caaccagtca cggttaatca gataatcaac ccccacctgc ccggcagctc
     1501 cccaggaatc tttcagactg agatcggaaa gccccgcctc tttgccatga tcgttaaatc
     1561 cattatcaaa gaaggtggtg tagttaatac ctgccccaac ataaggacgg aatttgctgc
     1621 tggcatcacc aaaataccac tgcgccatca gtgttggcgg cagatgatga acggttgcaa
     1681 tatcgccggt cgcccgggtg ccgattttat ggcggaacgg cgtcgctgcc agtaattcca
     1741 caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc cagttgagtg ttattggtca
     1801 cgctgaatcc acccagactt cctaacgtac caccagcacc ttctgttgga cgtacggttg
     1861 cagaacctgc acgcataaaa aattcgcctg cttcatgcgc aaaggcactg ccagagagaa
     1921 gagttgttac tgccaaagcc gccactgtta actttttcat atccgctccg tcgttatggt
     1981 tataaaaacg tgacgaatat acctacattg gagtaacaaa tgatcctaca tagatcacat
     2041 tacatatgat aattcaccat ttattgatct ggattaattt agaagttgca ttgagaatcc
     2101 tggttaacgt gatttaaatc aatttttaaa tattttcgct acgcaaatat taactcgttc
     2161 gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat tacctccgct ttacaaagat
     2221 atgcttttcc atacaagacc tgatgctgcc acccgaggct taaccagggt acaattgccc
     2281 gctaattaac acctgcataa aactcaagga gagtgcatgt ctatcacggc gcagtccgta
     2341 taccgtgaca ccggaaattt cttccgtaat caatttatga ccattctgtt ggtatcgttg
     2401 ctatgtgcgt ttatcacagt ggtgttaggg catgttttct cacccagtga tgcacagctt
     2461 gcgcagctca atgacggcgt gcccgttagc ggcagtagtg ggttgttcga cctggttcag
     2521 aatatgtcac cggaacagca acaaattttg ctgcaggctt cagcggcgtc cactttttca
     2581 ggattaatcg gtaacgccat tctcgccgga ggcgtaatat taattatcca gctggtgtct
     2641 gcgggtcaga gagtcagtgc gctgcgagct attggtgcca gtgcgccgat attgccaaag
     2701 ttatttattc tgatttttct gactaccctt ttagtacaga ttggcatcat gctggtggtc
     2761 gttccgggaa ttatcatggc catattactg gccctggcac cggtgatgct ggtacaggac
     2821 aaaatgggcg tttttgcctc gatccgtagc agtatgcggc tgacttgggc gaatatgcgt
     2881 ctggtggcac ccgcagtact gagctggttg ctggcaaaaa cactgttgct gctttttgcc
     2941 tcttcttttg ccgcattaac cccggaaatt ggtgccgtac tggcgaacac cttgagcaac
     3001 ctgatttcag ccatattgct catctatctg ttccgcctgt atatgttgat tcgccaataa
     3061 ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt tcttgatttt ttaccgctgg
     3121 ttgtcttttt cgcgttttac aaaatttatg acatctatgc ggctactgcg gcgctgatcg
     3181 tcgccacggc gattgtgctt atctatagct gggttcgctt tcgtaaggtt gagaagatgg
     3241 ccctgatcac ttttgttctg gtggtcgtct tcggtggctt gacgctgttc ttccacaatg
     3301 atgagtttat taaatggaag gttacagtca tttatgctct gtttgcggga gccctgttag
     3361 tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat gctgggtaaa gaactcacgc
     3421 tgccgcaacc ggtatggtcg aagctgaatc tggcctgggc tgttttcttt atcctttgcg
     3481 gtctggcaaa catctacatc gcattctggc tgccgcaaaa tatttgggtc aactttaaag
     3541 tctttggcct gaccgccctt accttaatct tcacattgtt aagcggtatc tatatctacc
     3601 gccacatgcc gcaggaagat aaatcctaac cagactgcca gtcctttggg gctggcgctg
     3661 tatctcctct gttctcctca ggaaaatcat agtagcatcg cgcctgtgat tttcctttta
     3721 agtcggtttt accatgtcta caacacataa cgtccctcag ggcgatcttg ttttacgtac
     3781 tttagccatg cccgccgata ccaatgccaa tggtgacatc tttggtggtt ggttaatgtc
     3841 acaaatggat attggcggcg ctattctggc aaaagaaatt gcccacggtc gcgtagtgac
     3901 tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg gtcggcgatg tggtgtgctg
     3961 ctatgcacgc tgtgtccaga aagggacgac atcggtcagc attaatattg aagtgtgggt
     4021 gaaaaaagta gcgtctgaac caatcgggca acgctataaa gcgacagacg cattatttaa
     4081 gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta cctgttgagt aatagtcaaa
     4141 agcctccggt cggaggcttt tgactttctg cttactgaat ttcggtggtg ccgttaattt
     4201 taaacaggat attcaccaca atcccactgc ctggcttacc cggctcataa cgccatctgc
     4261 gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg cttggctgag aggatttgta
     4321 cgttatccac gcgaccatcc ggcgtgacgt caaatttaac tttaacctgc ccttcaatgc
     4381 gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg gcttaatgcg cgtggtcctg
     4441 aagccacact ggtaaccggc ttgctggttg cagccgttgc tgtacttgat gtcgggcgtg
     4501 ccggtgccgt attttcaaac ggtgatgccg gacgcgactc tacgggtttg acatcgcgtt
     4561 tttgctgctc ctgtaccttt ttcaccggct tcggttttgg cttaggtttc ggcttcggct
     4621 tttcaatgac caccggtgct tcttttggcg gttcggggat cggctcaggt tccggttctg
     4681 gctctaccac cggctccggt ggcggctgaa cggcttgtgg cggttcgaga tcagcaggcg
     4741 caaccatcgt gacagaaatc ggctgcgcag gcgcaggtag ttcaataacc tgatgtaccg
     4801 aggtatagag cagacccgcc acaacagcac catgaatgca gaccgaaagt aacgtcggcc
     4861 aggggaagcg gcgaggtaaa tcaagggtca ttgaagtcat aatcatttca gtagaaaaac
     4921 caggtctcga ttttaaatgc aaatagcaat catattcaat aaggcaagac gatcaggatc
     4981 agtgaaaatg tcatttttgg cgtaattgac cttactttat gcatggtctt aacaaagaca
     5041 tcatctttcc gttgcagatc ttcgcccttt caaataacgt actttacaac tttcccgaac
     5101 aaggagttgt gcccgtgttg tatgttattt acgctcaaga caaagctgat agcctcgaaa
     5161 aacgcctttc cgttcgtccg gcacatttag cacgtttaca gttactgcat gatgaaggtc
     5221 gtttgttgac agctggtcca atgccagcgg tagacagtaa cgatccaggg gctgcgggat
     5281 ttactggctc aaccgttatt gctgaatttg aatcgctgga agcagctcag gcctgggccg
     5341 atgctgaccc gtacgtagcg gcaggagtct atgaacacgt ttcagtgaaa ccatttaaga
     5401 aagtgttctg agtagagatc caatatttaa gggcacggtt tttgcaaccg tgttcttgtt
     5461 tggcctttcc ttataggtcg gtcattgcat tcaccaccac aagcagataa taactttatc
     5521 tgtatttctt tatatgatgt gagcatgttc aggaaaaatg ccctttcctg acgcagagtt
     5581 gctcactcaa aacctttgat tactgcggat aaagtcatct actattgttt aacacattca
     5641 cgtgtcgcat gtatgttcag aaaatgtgca cgttaagata attaaacaaa attgtggtta
     5701 cagaggtatc agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga
     5761 ccctacggca cgacattctc gcgctggccg tctttttaaa tgggttgctt atttttaaaa
     5821 caatctatgg tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt
     5881 tggatctctc cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga
     5941 actccattgg cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt
     6001 tgatagcgct aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt
     6061 gcatttttac gctggtgttt ttactgatac tgcgcaaaga cttctcccac agtagcgccg
     6121 cagccgggac aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg
     6181 gtgcgcttta tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct
     6241 atttttcgat agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat
     6301 cagcacgatt gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta
     6361 tgacctcaat ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca
     6421 atcatacaat gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatta
     6481 atacgatcct gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg
     6541 aagatgatat caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg
     6601 acagtaatga cagttctgta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg
     6661 cgctgagtga taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca
     6721 gtgatgtcaa aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg
     6781 tacatccgga tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt
     6841 taaatggtga agagattaat aatgatatgc tcgtttcaat gttgttgaac tcaggtcatg
     6901 gtattttcag cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcggcgcaaa
     6961 aatagagttt aaagacggaa gagtaaactt attgggcacc gcaatggtgc ccagtacatt
     7021 tagatattgt agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg
     7081 atgctgtgga tacgcatatt tcggcgcgac tgcccctcaa gccaacgcga tttacgtcgg
     7141 ctagcctgac gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact
     7201 actctatgca ataacagaat ggtcattata cccccgcccc cgacgctgac cagtgctttt
     7261 cccgcgtttt tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa
     7321 aaggatttct aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga
     7381 tactggttgc tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc
     7441 tccgcgatgg cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat
     7501 cttgccgttg gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg
     7561 ccttccaccg caaaatggct taacgacctt aaagcctgta agcatatctt tgccgaagaa
     7621 aatagcagtg tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg
     7681 gtcaaaggga atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc
     7741 cgcgacatcc agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc
     7801 ggcatggcgg accaggtggc tgaatcatta atggcggctg cgcgacgcgg cattcattgc
     7861 cgattgatgc tcgactccgc cgggagtgta gcttttttcc acagcccgtg gcccgagcta
     7921 atgcgtaatg ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt
     7981 ttacgccgta tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg
     8041 tacaccggca gcatgaatat ggtcgaccct cgctacttca aacaagatgc gggcgtaggg
     8101 caatggattg atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt
     8161 tattcctgcg actgggagat tgaaacaggg aaacgtattc taccgccacc acctgatgtc
     8221 aatattatgc cgtttgagca ggccagcggt cataccattc acacaattgc ttctggcccc
     8281 ggctttccgg aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa
     8341 tatttgatca tgaccacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc
     8401 acggcggcgc agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg
     8461 ctggtcggct gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaagtt
     8521 tatcagtttg aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt
     8581 ctggttggca ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg
     8641 gcaatcgacg ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg
     8701 cgttcacgtc tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc
     8761 gagcgactgt tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca
     8821 ggtagtgact atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca
     8881 ggcctacgat atttttctcg aactggctgc cgacaatctc gatccagctg atgttctgct
     8941 gttcaatctt cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg
     9001 gcaggaacat gttgatttcg atttgaaccc agactttttt gccgaagttg tgattggtct
     9061 ggcggacagt gaagatggtg aaatcaacga tgttttcgcc cgcattctgt tatgccgcga
     9121 aaaagatcac aaactttgcc atatcatctg gcgggaataa ataatcactt tcggcgtgaa
     9181 aaagggctga cagctgtcag ccctttttgt gtttaaagcg gatcgacttt caggcaagaa
     9241 acggcgtgtc tgaaactccc ctccagcaca ggacgtgttt tggcacactc cggaccggca
     9301 atcgggcaac gggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc
     9361 ccttccagta actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc
     9421 gacatcaatg ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc
     9481 agttctaccg catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg
     9541 gccaggtcat gagcgataaa aattaatgac aatcccatct cacgttgcaa ttgctggagc
     9601 aggttgacca cctgtgcctg aattgacacg tccagcgccg acaccggctc atcacagata
     9661 atcagcttcg gttcaagaat aagagcacgc gcaatcccga tacgctggca ctgcccacca
     9721 gagaactcat gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc
     9781 gccttcacgc gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt
     9841 tcagcgatga tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa
     9901 atcatctgaa tatcactgcg aacggcacgc cattcatcgg gtttcatgcc cagcaactct
     9961 ttacctaacc aggcaacatg accgtcggtc gctttaacca ggccaatgat ggcgcgagca
    10021 aaggtggact taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg
    10081 cgaagagtta caccatctac ggctttgagc gttttcggcg gttgccagaa ccactgtttg
    10141 ccatctttga tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct
    10201 tcggttacag cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag
    10261 gcgtaaactc ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg
    10321 gctggaatgg gcaacctttc ggtaatcgca gcaggtttgg cggattaccc ggaatagtca
    10381 acattgtttc accttccgca tcgagacgag gcaccgcgtt gagcaaaccg atagaataag
    10441 gatgaacggg ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt
    10501 acattaccag cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtgatcataa
    10561 tgatggcggt attaaattcc cgcttcagtt cattcaatag cgtcatgatc tgcgcctgta
    10621 cggtgacgtc cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca
    10681 atgccatcgc aatcataact cgctgacgca tgccgccaga aaattcgtgc gggtacattt
    10741 tcatgcgttt acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa
    10801 acgcctcagc tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga
    10861 cgcgcatata gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag
    10921 cgcgcagttt attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg
    10981 ccgatccgcc aatgcgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg
    11041 atttacccga accagactca cctacaatgc ccagcgtttc tccggcacgt agggaaaaat
    11101 ttaaatcatt gaccgccgtg acgtcgccgt ccggggtact aaaggtgaca cgcaaatctt
    11161 tcacgttcag cagtgcgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta
    11221 cgctcatggc tgcactcctt aacgatcttt cgggtcgagg gcatcacgca agccatcgcc
    11281 gataaagttg aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acagtaacca
    11341 tggagagact tccattgagt tcgcgccatc actcagcaat gccccccagc tgcttaacgg
    11401 ctcttgcgta cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac
    11461 cagtagcgat gcgtagacca ccaccacacc gagtacgttc ggcacaatgt ggcgaataac
    11521 aatgcccgac gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag
    11581 actcagggtt tgcccacgca caatacgagc catatccagc caggaaacca tgcctatcgc
    11641 cacgaaaatc agcaggatgt tttgaccgaa aaaggtcacc agcaaaatga cgaagaacat
    11701 gaatgggaag gagttgagga tttccagcaa acgcatcatt accgaatcca ctttaccgcc
    11761 cagataaccg gaaagcgaac cataaagtgt ccccacgacc actgccacca gtgccgcagc
    11821 aacaccgacc atgagtgaga tacgtccgcc aatcgcaacg cgcacaagca ggtcgcgacc
    11881 ggatgagtca gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc
    11941 ccagtcagta tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc
    12001 gatcagcacc agcactatca gacttgcaac cgccgcacgg ttatgcataa aacgtcgacg
    12061 tgcgtcctgc cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt
    12121 ctcgctgttt ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg
    12181 tcgataaccg catatagcac atcgacaatg gcattaaaca aaatggttaa cgcaccaacc
    12241 aggatggtca ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt
    12301 tgcccaatcc ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg
    12361 acaaatgcgg ggcccatata ggagagcacg ggtaacagag caggttttaa tgcgtggcgt
    12421 aaaatgatcc gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt
    12481 aatacttcaa tcatagagcc acgggtaata cgcgcaatac tggcgatata ggcgagtgac
    12541 aacgccacca tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc
    12601 agccaatgca aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg
    12661 ataacaaccc cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt
    12721 aatgcggcaa taacgccagc actaacaccc agtattaccg caaggaaaaa tgctgcggct
    12781 cccagtttgg cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta
    12841 tatttaaacg atggaccgaa atcgccatgc gccagttgtt tcaggtagct gaaatactgt
    12901 gtcatgatcg gatcattaag atgatatttc gcttcgatat tggccattac ttccggcggt
    12961 aaagtacgtt cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata
    13021 gtaataagaa taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac
    13081 attgccctac cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc
    13141 aggacactcc tgccccacgt attgccatca gtgcttcaca atgtacatat tccgggtgta
    13201 ggtattatcc agcggatctt tgccggtata accaccaacc cacggtttca ccagacgcgc
    13261 attcacgtag taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt
    13321 agtgtacaga gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct
    13381 gtcaaaggcc gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt
    13441 caggaaggaa gttggttcgt tgtagtcagc acaccagcct gcacgggcca catcaaaagt
    13501 accctggtga cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac
    13561 accaatgttt ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcgga
    13621 ggtgttatac aacaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag
    13681 cagttttttc gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ccggctgagt
    13741 caattttgcg ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc
    13801 tttcacttta ttaacaatga tatcgcggtc catacccagt ttcagtgcgg tacgcacacg
    13861 cacatcgttg aatggcggtt tctggttgtt aatttcgtaa taataagtgc acaggtatgg
    13921 atcaacgtga acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat
    13981 gttgttataa gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga
    14041 agcaatgggc aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggttgg
    14101 gctgcgttca agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt
    14161 ggtgacgata ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg
    14221 caccggtgaa gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa
    14281 ggtgacttct aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt
    14341 cccttcaaga atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg
    14401 agaagcggtg ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac
    14461 tggcgtgcca tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc
    14521 tttattatcc caggattcag cgacgccagg tgccggatgg ccgtcaagat cgctgaccag
    14581 taagccttca aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg
    14641 atctaatgac tgaacttctg aaccattgtt acgtaccagc gtttgttttt ccgccagtgt
    14701 gacgcctgcg ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac
    14761 gccagctgct actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt
    14821 ataattactg gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga
    14881 gactttctgc tgtcaggtta ctttatcgtt acggattgct atcaccgact ttatttattc
    14941 tggtgattca gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat
    15001 tattctttcg cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgcctg
    15061 acggttgccc ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt
    15121 taagcaattc tcttttattg accgtatcag aatctatagc ttgcatccat gtcttaacaa
    15181 acatctttaa attcaatcaa atagagattg gttaagcatg tcgatctttt ctgattacag
    15241 ttccagtagt gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac
    15301 caaaggcagt ggaattacta atatcatttc aattattctt cagcaagccc aggattatga
    15361 tgtggcgaaa ataacatgat gatgaggaga agcagttaag gaaatcctta taactaactg
    15421 taatttgaga agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa
    15481 gtaaaaataa tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa
    15541 ttaagcaggc cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc
    15601 atcagcaata gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt
    15661 accagccacg gtgccatgcg gaacaatccc caacaacata aagcgaacaa tgcaatagcc
    15721 acaaagaaac caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa
    15781 ctgatcgccc ctggccccgc catcaacggc aacgccaatg gcaccacacc aatactttca
    15841 cgtaccgcgg tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg
    15901 atcatcgaca tcgctatcgt caccaccagg atgcccccgg cgatacggaa cgaatcaatt
    15961 gatataccaa aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg
    16021 gccacagaca ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg
    16081 gtcatgctga taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg
    16141 aaaaatttga agtaaacggg aaaatcaaaa agggtctgaa tcacggttag ctccgaagca
    16201 aaagccggat aatgttagcc ataaataagg ttgaaaagac gcgctgacaa tacgcctttt
    16261 gacagcattt ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt
    16321 tgtgcaaaac atgctaatgt agccaccaaa tcatactaca atttattaac tgttatctat
    16381 aatggcgaaa agcgatgctg aaaggtgtca gctttgcaaa aatttgattt ggatcacgta
    16441 atcagtaccc agaagtgagt aatcttgctt acgccacttg gaagtgacgc attagagata
    16501 ataactctaa tgtttaaact cttttagtaa atcacagtga gtgtgtgcgc gagtaagctt
    16561 ttgattttca tcggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag
    16621 atgatttact aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc
    16681 tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac
    16741 tcaagagcaa gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat
    16801 cccactcgcg aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat
    16861 caaaaaccac tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg
    16921 tgttctgtct gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat
    16981 ttgcggtatc gttccgacca ctaacccgac ttcaacagct atcttcaaat cgctgatcag
    17041 tctgaagacc cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgcgaccaa
    17101 caaagcggct gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat
    17161 cggctggatc gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat
    17221 caacctgatc ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa
    17281 accagctatc ggcgtaggcg cgggcaacac tccggttgtt atcgatgaaa ctgctgatat
    17341 caaacgtgca gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc
    17401 ttctgaacag tctgttgttg tcgttgactc tgtttatgac gcagtacgtg aacgtttcgc
    17461 aacccacggc ggctatctgt tgcagggtaa agagctgaaa gctgttcagg acgttatcct
    17521 gaaaaacggt gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact
    17581 ggcaggcttc tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga
    17641 tgaaagcgaa ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa
    17701 agatttcgaa gacgcggtag aaaaagcaga gaaactggtt gctatgggtg gtatcggtca
    17761 tacctcttgc ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca
    17821 gaaaatgaaa acggctcgta tcctgattaa caccccggct tctcagggtg gtatcggtga
    17881 cctgtataac ttcaaactcg caccttccct gactctgggt tgtggttcct ggggtggtaa
    17941 ctccatctct gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg
    18001 agctgaaaac atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct
    18061 gccaatcgcg ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga
    18121 ccgcttcctg ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg
    18181 cgttgaaact gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa
    18241 aggtgcagaa ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc
    18301 cccgatggac gccgcgaaga tcatgtgggt tatgtacgaa catccggaaa ctcacttcga
    18361 agagctggcg ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg
    18421 cgtgaaagcg aaaatgattg ctgtcaccac cacttctggt acaggttctg aagtcactcc
    18481 gtttgcggtt gtaactgacg acgctactgg tcagaaatac ccgctggcgg actatgcgct
    18541 gaccccggat atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg
    18601 tgctttcggt ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc
    18661 atctgagttc tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc
    18721 agcgtcctac cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc
    18781 gactatcgcg ggtatcgcgt ttgcgaacgc cttcctgggt gtatgtcact caatggcgca
    18841 caaactgggt tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa
    18901 cgttattcgc tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga
    18961 ccgtccgcag gctcgccgtc gttatgctga aattgccgac cacctgggtc tgagcgcacc
    19021 gggcgaccgt actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaggc
    19081 tgaactgggt attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc
    19141 gaacgtggat aaactgtctg aagatgcatt cgatgaccag tgcactggcg ctaacccgcg
    19201 ttacccgctg atctccgagc tgaaacagat tctgctggat acctactacg gtcgtgatta
    19261 tgtagaaggt gaaactgcag cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc
    19321 gaaaaaatcc gcttaatcag tagcgctgtc tggcaacata aacggcccct tctggggccg
    19381 tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct tcatcatatt
    19441 tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca caagtcaacg
    19501 tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaactgccga gaagggtgta
    19561 tagcccggaa gaagtgcgta aaacgaactg acaggataaa agtgccctgc tcaccctgtc
    19621 agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg aatagccgtt aatgagccga
    19681 cttgtaacgc ctctttatag tgtttacggc atacagaaac gtatcgttca ttaccaccaa
    19741 taaccacctg ctcaccttcg ttataaggtc tgcctgcttg atcaagacgc agcaccatgc
    19801 ttgctttacg gccacaaaaa cagatggttt ttaattcaac cagtttgtcg gaccatgcca
    19861 gtaagtattg gctgccaata aataattcac ctcgaaaatc ggtacgtaaa ccataacaaa
    19921 gtacgggtat atcgagttga tcgacaacct ccgataattc atatacttgt tgtctggtta
    19981 aaaactggca ttcatcaacc agtacgcaat gaattgcctg ctgctcatgt tccgcacgaa
    20041 tctcatcaaa taatgatgaa ttttggttaa ataattttgc aggcgatgac aaacctatac
    20101 gcgaactgac tttcccggca ccaaagcgat catcaatttc tgccgtatat acgacagtgc
    20161 gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa tgctgtagac ttacccgcat
    20221 tcattgcgga atagtagaaa tatagctgtg ccatcagcca caggccctca atgatatgaa
    20281 ataaagttgg ctggagttta tcataattcg tcagtttttc agctcaacag tatgcacaga
    20341 agtggtatat gcgttctccc ttacgaagcc ttgcataatc cttctgagct atcattacaa
    20401 ctgcctcgct tgttataagc gggtaaatga ctgctggtaa ctattcacaa tctttaacct
    20461 gttgcgcaag taatagccct ctgttgacct ccaggagata gtgcaatact aagtccctgc
    20521 tcttattgcg actgttctac ttttcatcat tcgcttaata gggaattctc gtaaacacga
    20581 ctaatacaga agactgaaag gtcgtcagcc tacgataatc tccccataaa atgtgacatg
    20641 aatcaggaag ttttaacctc acgtgctgcg aaatcatcgg tacaaatagg gctatatgcc
    20701 gcgtcttttc tggctaattt tatgaaaaga tatttattgg cggcacaaaa taaagaacaa
    20761 ttttgaattc cttacattcc tggctattgc acaactgaat ttaaggctct attattacct
    20821 caacaaacca ccccaatata agtttgagat tactacaatg agcgaagcac ttaaaattct
    20881 gaacaacatc cgtactcttc gtgcgcaggc aagagaatgt acacttgaaa cgctggaaga
    20941 aatgctggaa aaattagaag ttgttgttaa cgaacgtcgc gaagaagaaa gcgcggctgc
    21001 tgctgaagtt gaagagcgca ctcgtaaact gcagcaatat cgcgaaatgc tgatcgctga
    21061 cggtattgac ccgaacgagc tgctgaatag ccttgccgcc gttaaatctg gcaccaaagc
    21121 taaacgtgct cagcgtccgg caaaatatag ctacgttgac gaaaacggcg aaactaaaac
    21181 ctggactggc cagggccgta ctccagctgt aatcaaaaaa gcaatggatg agcaaggtaa
    21241 atccctcgac gatttcctga tcaagcaata atcttttgta gattgcactt gcttaaaatc
    21301 ccgccatcgg cgggattttt tattgtccgg tttaagacaa tttaataaga ataagctata
    21361 aaaaaacggc gtcgattgct caacgccgtt tcgtggataa caccgatacg gatgttactt
    21421 cttaatgccc atctcttctt caagccaggc tttaaattcc gtgccaaggg tgttatgacg
    21481 aataccgtat tcaacgaagg cctgcatgta acctaattta ttaccgcagt catggctctt
    21541 ccctttcata tgataggctt caaccgtttc tttttcgatt agcatatcaa ttgcgtcggt
    21601 gagctgaatt tcatcaccag ctcccggagg ggtttttgcc agcaacggcc aaatatccgc
    21661 actaagtacg taacgaccca caatagcgag attagacggc gcaacatccg cttttggttt
    21721 ttcaaccacg ccaaccatcg gtacgctttc acccggcgct aattcaacgc ctttgcaatc
    21781 cacaacgcca tatgcggtca catcagcaac cggttcaacc atgatctggc tatgacccgt
    21841 ttcatcgaag cggcggatca tctctgccag gttatcctgt gacaaatcgg attcatattc
    21901 atccagaata acgtcaggca aaataacagc cacaggttca tcacccacta ccgggtgagc
    21961 acacaatacc gcgtgtccca ggcctttcgc cagcccctga cgaacttgca taatagttac
    22021 gtgcggcgga caaatagact gcacttcatc aagcagttga cgttttacac gtttttccag
    22081 cattgcttcc agttcaaaac tggtatcaaa gtggttttca atagagtttt tagatgagtg
    22141 tgtaaccagc acaatttcag taatgccagc cgcaatacat tcattcacga cgtattgaat
    22201 taatggctta tcgacaagtg gcagcatctc tttcgggatg gctttcgtcg ccggcaacat
    22261 cctggttcct aatcccgcaa cggggataac ggcttttttg actttcgtat taatggcagc
    22321 catttaaatt ctcctggact gttcgtgttt tgaacgtgtt catatttctg tatcgcatcc
    22381 cagtatatca gcaccccgga ggggctaagt tctgaaaagg atgcgagtta gcataattaa
    22441 gacaattact tatacgaatg gcgacgatag taccaccagc cgacattagc tggtaatgca
    22501 aatttagccc gcgttatcgt ttgctcattc tgcagacaac atcaagcgca gtcgaccacc
    22561 ggttccccat atttggcatt gccaggcatc gcatcgctgg ctcaattgat tcaaataagc
    22621 gttacctaaa gtgcctaacg gaacaccatt actgatttgc acctggtgtt cgccggtatt
    22681 taacgtcgca ttcagacccg cagaaaccag aatgagattt ttcagttcgc gatgataata
    22741 gccaactaat agcggaaact gccccggcag attggcctga cggagtaaat ggtttacctg
    22801 cttcaataac gcgcccaact ctggcaaccg ttgattttgg tgtgcaagct gttcctgtaa
    22861 taatccgtta aacaatgcgc gtaataacaa ggcagcaagt acgccattat gtccagctcg
    22921 ggttacatca aggcaataaa atgccagatc gttttccgaa agtgcggcaa tatcaagcac
    22981 caggccgggt ttgtccgcgg caaccaattg acggtaatta acccggcaat gggaaatcac
    23041 ctgctgaacc ggcggttgta gttcctgtaa taatttcgcc gctgcggcag ggttatcaac
    23101 cattgcatcc cagtcgcgaa aaagcctttc ctcttcctca acgcgcgaat taaacatgct
    23161 gggatagaga caggcaaaaa ccatctcgcg caagcgattc agatctttaa ctggtttcag
    23221 caaaacatct tcaacgccca gacgtaacgc tttggcaata tctgccatat tttcagtggc
    23281 agatatcacc agaactgggg tctggtcgcc tctgttacgt atatgctcca gcagtttaag
    23341 cccgttcatt cgtggcatcg cgatatcaca tatcatcagg tctggagtga aacctcccag
    23401 caactcaagg gcatccaccc catcagccgc cagtaccgtt gtcgctccca atgaggaaaa
    23461 ccacgaatcc agaagcgagc gaaatacctg ctcatcttca acaatgagaa tctgttttcc
    23521 gaccaatggc tgcgtcatgt tctctcccct gactggcttt actcaatagt ggcatgctat
    23581 tgccacctgc gcctgtcaga attaacttaa gtgtagataa aaattctggt caaatgttgg
    23641 tgcgtaccaa cggcaaaagt tcgtccattt tcttttccac tgccagctgc ccggccgcaa
    23701 tggcagcgtg cgcgcgatgg aaatcaagcg tagatatttg cgggcaaaca ggttgaatca
    23761 gaatatcggg cggatcacct gccatgcggt tccttttaag gcggttctcc agcacctgga
    23821 tagaagtggt cataatctct gttgccgttg gtgctgtcac cgcccgacgt gtcgttatac
    23881 tgcctaacct ttctttcaga cgcgcatgcc acggcagaga atcaccattc tcgctatttt
    23941 cttcactgac attaaaggag agcaaatctt gttgcatcaa atgagcatcg tgctgcaggt
    24001 cgaccgctat cacaatatca gcacccaatg cacgcgtgag cgaaatagga attgggttaa
    24061 cgactgcccc atcaaccagc cagtagccgt tatgcgcaac gggtgccatt agtcctggaa
    24121 tactgcatga tgcgcgaata gcaagatgga gatcgccttc agtaaaccat aattcacgcc
    24181 cagtacttaa attggtggca acagccgcga agcgacggga acaattttcg atctctgttt
    24241 ccggcattat ttcgcggtat tgattgaaga cacgctcgcc gcgcagtaac ccaccgcgct
    24301 gccaggagag atccatcagt cgtaaaacat cccaataact gaaagaggtc acccaatctt
    24361 ccagcgcaga taatcgatcg catgcatagg ccgcgcccac cagcgaacca attgaacatc
    24421 ctgcaacgat atcaatttca atacccactt tttttagcgc attaataacg ccaatatgcg
    24481 accaacctct cgccgcgcca gatcccagcg ccagccctat ttttatcttt ctcattattc
    24541 ccgcaagatt cccctggaat gcaatcgtag ccacatcgct actgctcggt taacatagtg
    24601 ctactcaggc ggttaatacg ccgttattgt ttccagggag attatttgtg tctcagcttt
    24661 gtccctgtgg tagtgctgtc gagtatagcc tatgttgcca cccttatgtg tctggtgaaa
    24721 aggttgcacc tgatccagaa catctcatgc gttcgcgtta ctgcgctttt gtgatgcaag
    24781 acgcagatta tttaataaag acctggcatc catcttgtgg agcagcggcg ttacgtgccg
    24841 aattgattgc cggatttgcg catactgaat ggttgggatt gaccgtattt gaacattgtt
    24901 ggcaggacgc cgacaatatc ggatttgtca gttttgttgc tcgatttacc gaaggtggca
    24961 aaacaggtgc aataattgaa cgttcgcgat ttttgaaaga aaacggtcaa tggtactata
    25021 ttgacggtac acgtccgcag tttggccgca acgatccctg cccttgtggt tcaggtaaaa
    25081 aatttaaaaa gtgctgcggc caataatggt tgacggtacg gtttagcaaa cactctcaac
    25141 aaggtttttc cagcaatgca ttcactccaa cgtaaagttc tgcgtactat ttgtccggac
    25201 caaaaaggtc tgatcgcacg tattaccaat atttgctaca agcacgagtt aaatatcgta
    25261 cagaacaatg aatttgttga tcaccgtacc gggcgctttt ttatgcgcac ggaactggaa
    25321 gggattttta atgattccac cctgctggcg gatctcgata gcgcattgcc agaaggctcc
    25381 gtgcgtgagc tgaatcctgc cggtcgtcgc cggatagtga ttctggtcac taaagaagcg
    25441 cattgccttg gcgatttgtt gatgaaagcc aattatggcg gcctggatgt cgaaatcgcg
    25501 gcagtgattg gtaaccacga tactttacgt tctctggttg agcgttttga tataccgttt
    25561 gagctggtaa gccatgaagg gttaagccgc aacgagcacg atcaaaagat ggcggatgcc
    25621 attgatgctt atcaacctga ctacgtggtg ctggcgaagt atatgcgggt attaacaccg
    25681 gaatttgtgt cacgcttccc gaataagatc atcaatattc accattcctt cctgccagcg
    25741 tttatcggcg cacgtcctta tcaccaggcc tatgaacgtg gcgtgaagat tattggcgca
    25801 accgctcact atgtgaatga caatctggac gaaggcccaa tcatcatgca ggacgttatt
    25861 catgtcgatc atacctacac agctgaagat atgatgcgcg caggtcgtga cgtcgagaaa
    25921 aacgtcttaa gtcgcgcgct ctacaaagta ctggcgcagc gcgtctttgt ttacggtaat
    25981 cggacgatta ttctttaatc gccagcaaaa ataactggtt acctttaatc cgttacggat
    26041 gaaaattacg caaccagttc atttttctca acgtaacact ttacagcggc gcgtcatttg
    26101 atatgatgcg ccccgcttcc cgataaggga gcaggccagt aaaaagcatt accccgtggt
    26161 ggggttcccg agcggccaaa gggagcagac tgtaaatctg ccgtcatcga cttcgaaggt
    26221 tcgaatcctt cccccaccac catgattcac cacagcgatg tggtaacttc ggtgagattg
    26281 tgaaagtttg cactcatcgg ggaagggtga gaaccttcga ctaaggttcg actcgagcga
    26341 aagcgagaga acgttttcac aggcaacggc ccgaagggtg aggagcgaag cgacgaataa
    26401 tcctttcccc accgccatct caagcattac ctcaaaattt gaattacccc tggtggggtt
    26461 cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga aggttcgaat
    26521 ccttccccca ccaccatcac tttcaaaagt ccctgaactc tcaagcgaat ccgtaaataa
    26581 atattctgcc caagcggaga aggatgagaa gcttcgacta aggttcgact cgagcgccag
    26641 cgagagagcg ttgccgcagg caacgacctg aagggcgaag cgcgcagcgc tgagtaatcc
    26701 ttcccccacc accatcactt tcaaaagtcc ctgaactctc aaacgaatcc gtaattaaat
    26761 attctgccca agcggggaag gatgagaagc ttcgaccaag gttcgacttg agcgccagcg
    26821 agagagcgtt gccgcaggca acgacccgaa gggcgaagcg cgcagcgctg agtaatcctt
    26881 cccccaccac catcgcttcc tgaaataatc aaaacctgtt actccgagct aattttgccc
    26941 aatggcacca cgtttaccgg acctacaata tcctcccttg taggtcggat aagcgaagcg
    27001 catccgacaa ctcaccccgc accgtgccca taaaaaaacc ccgccgaagc agggtctatg
    27061 tgaactaaaa ttcgcttagt gacgagcgcg caccagctgg tacttacgtg tcagatactc
    27121 caccggtacg ctccagatgt gtaccagacg cgagaacggg aacagcagga ataacgtcat
    27181 ccccagcacc aggtgtagac ggaagataaa cgccacacca tcgaggtgtt gagaagcgcc
    27241 accgtggaag gtcactaccg actgcgccca gccaaccagt ttcatcatct cgctaccgtc
    27301 catatgttgg gcggagaacg gaatggtcaa caggcccagc gcgcactgga taacgagcag
    27361 cgacaggatc agtatatccg ctccggtagt cgttgcacgc acgcgtgggc tgaacagacg
    27421 acgtttaagc aaaagcacgc cgccaatcag acacagcacg ccgctggcac caccagcaaa
    27481 catcgccatt ttctgtttca cttcaatcgg cagccaggct tcatacatcc agtgcggcgt
    27541 caacataccg aagaagtgac cgacaaaaat ccccagaata ccgatatgga acaggttcga
    27601 cgccaggttc atccctttgc gatccagcat ctggctggac gccgcgcgcc aggtgtactg
    27661 cccgtagtca taacgcagcc agctaccaat caggaagacc gccccggcta tgtacgggta
    27721 gatatcaaag aagaacatat tcaggaattg cattagtgct gtcctccggt ggtgatattc
    27781 agatattgcg gcgcgacggc accggcaaag cgacgctgat gagcagtgat tgctgaatca
    27841 ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt cccaaacagc atccagcgcc
    27901 tgcggcgtat catcgcgcgc ttcgtcggca attttttccg ccactttgtc gctgtcgata
    27961 gcggtattgg ccaatttcag cagcagatca aacagcacgg cataacggct ttcacgctgt
    28021 tgcagacgcg cactcagcaa tgccagaatc ggcgcgatat ctttcaaacc ttccacggct
    28081 tcgctttgcg gcagctgcgc caggtactcc agatacagcg gcagatgatc cggcaattcg
    28141 cggctgttta actgcaagcc gtgctgctcg tactgcgcca gcaggtccac catcgcctgg
    28201 ccgcggtcgc gggattcgcc gtgcacatgt tcaaacagca acagtgacgt ggcgcggcca
    28261 cggtcgaaca gttcgctgta ctgcgcctgg gcatcgagcg gatccatcgt cgttaaatcg
    28321 cgcaggaaaa tgcccagcgc atgggcatcc tcttttggca gatttttcga cgcggcaatc
    28381 gcctcaaaca tctcttgttg atgctgccat aaggcagcat ccggatattc aaggagacgc
    28441 gatacaatca cgagttcgat catggatgcg gctccgtttt gctggtcaca tcgatggcat
    28501 cgatacgacg gctgttgaac agattgaatt tggtatctga accgtggcag ccatcaccaa
    28561 aggtaaagcc gcagccattt ttctccggga aggcttcccg tgccagttca cgatgactac
    28621 tcggcaccac aaagcgatct tcgtagttag caatcgccag ataacggtac atctcctgtg
    28681 cctgggcttc ggtcagaccg acctcttcca gcgcacgggt atcaacttta ccgtcaacgg
    28741 tttcagcacg tttgtaatga cgcatcgcca gcatacgttt cagcgcgcgc agtaccggtt
    28801 tggtatcacc ggcggtcagc agattcgcca gatactgtac cggaatacgc aggctttcga
    28861 cgtctggcag aatgccgttg ctacccagct cacccgcgtc tgctgcagac tgaatcggag
    28921 agagaggcgg cacgtaccag accatcggca gtgtgcgata ttctggatgc agcggcagcg
    28981 ccagtttcca ttccattgcc attttataaa ccggcgactg ctgtgcagct tcaataacgc
    29041 tcagcggaat accgtcttta atcgcctgct cgatgacttt cggatcgttc ggatcgagga
    29101 acacctccag ctgacgctgg tagagatctt tctcgttctc ggtgctggct gcacgttcaa
    29161 tcgcgtcggc atcgtacagc agcacgccaa gataacggat acgaccgaca caggtttctg
    29221 agcacacggt cggctgaccc gcttcaatac gcggatagca gaagatgcac ttctcagact
    29281 taccgctctt ccagttgaag tagatttttt tgtacgggca tccggtgatg cacatacgcc
    29341 agccacggca tttatcctgg tcgatcagga cgatgccatc ttcttcacgc ttgtaaatcg
    29401 caccgctcgg gcaggtcgcc acacatgccg ggttcaggca gtgttcgcac aggcgcggca
    29461 aatacatcat gaaggtgttt tcgaactggc tatacatcgc cttctggatg ttgtcgaagt
    29521 tcttgtcttt cgccagtttg tcaaactcac cgcccaggtc atcttcccag ttcggccctt
    29581 tttcgatttt cgccatccgt tccccggtaa tcagcgaacg cggacgggca atcggctgcg
    29641 atttgctgcc ttccggcgcg gtatgcaggt tctgatagtc aaaatcgaac ggctcgtaat
    29701 aatcgtcgat ccccggcaga tgcgggttag cgaagatttt acccagcagc atggcacggt
    29761 tacccatgcg cggttgcagt ttgccgttga ttttacggat ccagccgcct ttgtattttt
    29821 cctggttttc ccagtcagtc gggaagccct ggcccggctt ggtttccacg ttgttgaacc
    29881 acgcgtattc cacgccttca cggctggtcc agacgttttt acaggtaact gaacaggtgt
    29941 ggcagccgat gcacttatcg agattcagca ccatgccgac ttgtgaacga attttcattt
    30001 tacgctctcc tgtacctgat cattgccttc gccatctaac cagtcaatgt tcttcatctt
    30061 acgcactaca acaaactcat cgcggttaga acctacggtg ccgtagtagt taaagccgta
    30121 tgccagatgg gcatagccgc cgatcatatg cgtcggtttc ggcgtgatac gggtgaccga
    30181 gttatggata ccaccacgct gttgggtaat ttccgaacca ggcaggttaa cgatacgttc
    30241 ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc tggctgacaa ccgcacgggc
    30301 agtcagagca ccgttgctgt tgaatacttc aatccagtcg ttatcggcga tacccagatc
    30361 tttggcatcg gcttcactca accagaccac cggaccaccg cgacctaaag tcagcatcag
    30421 caggttgtcg ctgtaggtgg agtggatacc ccacttctgg tgcggcgtca ggaagttgag
    30481 cgctttttcc gggttgccgt tggatttctg gcctatcact tctttcaccg aacgggtgtc
    30541 gatcggcgga cgataaacca gcaggctttc accgaaatca cgcatccact ggtgatcctg
    30601 atacagttgc tgacgaccag agagcgtacg ccatgggatc agctcgtgaa cgttggtgta
    30661 accggcgttg taagaaacgt gttcatcttc cagaccagac caggtcgggc tggagataat
    30721 tttgcgcggc tgtgcctgaa tatcgcggaa gcggatcttc tcgtcttctt tattcagcgc
    30781 cagatgcgta tggtcacgac cggtaaattc gctcagggca gcccaggctt tcacggctac
    30841 ctgaccgttg gtttccggtg ccagtgtcag gatcatctct gccgcatcaa ttgcggtgtt
    30901 cagcatcggc tggcctttcg ccggaccttc cgctttggtg tagttgagct tacgcagcag
    30961 atccatctcg ctctgggtgt tccaggcaat ccctttaccg ccgttaccga ttttctccat
    31021 cagcgggccg atagaggtaa agcgttcgta agttgccgga taatcgcgct ctacgaccat
    31081 aatgtgcggt gcagttttgc ctgggatcag gtcgcattcg ccttttttcc agtctttcac
    31141 atccagcggc tgcgccagtt cagcggcaga gtcgtgctgg ataggcagcg tgacgatgtc
    31201 ggtttcttta cccagatggc cgacgcacac ttcggagaat ttcttcgcga tggctttgta
    31261 gatttcccag tcgcttttcg cttcccaggc cggatcgacc gccgcagaca gcgggtgaat
    31321 aaacggatgc atatccgaag tattcatgtc gtctttttcg taccaggtcg ccgtcggcaa
    31381 aatgatgtcg gaatagagac aggtgctcga cagacggaag tccagcgtaa ccaccagatc
    31441 cagcttgcct tccagaccat tgtcctgcca gtccacttct tccggcttca cgccgccctg
    31501 ctgccccaga tctttaccct ggataccgtg ctccgtcccc agcaggtact tgagcataaa
    31561 ctcatgacct ttaccggaag aaccgagcag gttagaacgc cagataaaca ggttacgcgg
    31621 gtggtttttg ccgttttccg gctgttccgc agcgaaacgg atagaaccct ctttcaggga
    31681 tttcaccgta tagtccaccg gattcatccc ggctttttct gcttcgccag cgatagtcag
    31741 cgggttagtg cctaactgcg gcgcagacgg cagccagccc atgcgttctg cacggacgtt
    31801 aaagtcgatc agatgtccgg tatagcggga tttatccgcc atcggcgaca gcagttcttc
    31861 cgcagtcacg gtttcataac gccactggct ggagtggtta tagaaataag aggtgctgtt
    31921 catgtgacgc gccggacgct gccagtcgag ggcaaacgcc agcggctgcc agccggtttg
    31981 cggacgcagt ttttcctgac ctacatagtg cgcccagccg cccccgctct gaccgacaca
    32041 gccgcagaaa atcagcatgt tgatcagacc acgatagttc atatcgaggt gataccagtg
    32101 gttcagcccc gcaccgacga taatcatcga acgaccgtgc gttttatcag cgttatcggc
    32161 aaattcacgg gcgatgcgaa taatctggct gcgagaaacg ccggtaatct gctcggccca
    32221 ggccggggta taagctttca catcgtcata gctggttgca cagttaacgt cgttcaggcc
    32281 acgttccaga ccgtagtttg ccagcgtcag atcataaacg gtggtcacca gggcggtgct
    32341 gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc agcagcacgt tttccagttc
    32401 cactttgttg aagtgttccg tgccgtcgcc accaaagtac gggaagccta cctcagcgat
    32461 ctcatcctgg ctacccagca ggctcagttg cagctcggtt tcttcgccag ttttgccgtc
    32521 gcgctgctca agattccatt tgcccttctc gccccagcgg aagccaatag aaccgttcgg
    32581 cgcaaccatt tcgccattgg tattaaaggc gacagttttc cattccggat tgttttcctg
    32641 gcccagcgca tcaaccagat cagcagcgcg cagcatacga cctgcagcgt agtaaccgtc
    32701 gcgttcttcc agcatcacca gcatcggcat gtcggtgtag cgacgcacat agtcggtgaa
    32761 atactggctt gggttgtcga gatggaattc acgcagcatt acgtggccca tcgccagcgc
    32821 cattgccgca tcggtgccct gtttcggtgc cagccacaga tcgcacagtt tggcgatttc
    32881 agcgtagtct ggtgtgacgg caacagtttt ggtgcctttg taacgcactt cagtaaagaa
    32941 gtgagcatct ggggtacgcg tctgcggcac gtttgaaccc caggcgatga tgtagctgga
    33001 gttgtaccag tcagcagatt ccggtacgtc agtttgctcg ccccaggttt gcggagacgc
    33061 aggaggcagg tcgcagtacc agtcgtagaa gcttaagcaa gtaccgccaa tcagcgagag
    33121 atagcgtgca cccgatgcgt aagaaaccat cgacattgcc ggaattggcg agaaaccagc
    33181 aacacggtcc gggccgtagt ttttgatggt gtaaacgtta gatgcggcga tcagttcgtt
    33241 cacctcctgc caggaagaac gaacaaaacc accgcgtcca cgcgcctgct taaagctttt
    33301 cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca accggatcgc tatgcagcgc
    33361 cttcgcttca cgccacattt tcatcaggcg tttgcgcatc atcgggtatt tcaggcggtt
    33421 ggcactgtaa agataccagg agtagctggc accgcgcggg cagccgcgag gttcatggtt
    33481 tggcagatcc ggacgggtac gcggatagtc agtctgctgg gtttcccagg tgaccagacc
    33541 gtttttgacg tagattttcc agctgcagga gccggtgcag tttaccccgt gggtagagcg
    33601 gacgattttg tcatgctgcc aacgctggcg atatccatcc tcccagtcac ggttggtatt
    33661 gagaagctgg ccatgcccat cggcaaaggt ttcacccttc tgtttgaagt agcgaaaccg
    33721 gtccaggaat ttactcatcg gttttctcct gtgggagcct gtcggccctc tgataaatcg
    33781 acattgctaa tgtttaaggg caaggtaacg ctctgaaaca gcgtgggaat tgataacgat
    33841 caagagtgat ggggatgaaa aataaagtaa attcctaaga taccccttta ggataattaa
    33901 cagttacagc ataacatgct gtaaattaag acgttttata cctgtagtta tttaaactat
    33961 taagattttt atgcaaatgg gtattaagga gtattcccca tgggtaacat attaatggtt
    34021 cttaatgaaa tccggcgaaa taaaccatat aacattagta tgttgtaact aaatgacgca
    34081 taaaaaaagc gcgacatcat gccgcgcaaa ggataatcaa atattaagat ttatttttta
    34141 gaatgccgac catataccgc ccaggtaatc actacgcagg cgatatagaa aatcaaaaat
    34201 actttcattg cgccgactgg cgaacccgtt aatgccagcg agctaccgaa cgctttcggg
    34261 ataaagaagc caccaatcgc gccaatcgca gagatgaaac ccaacgccgc cgccgtgtcg
    34321 gttgccgctt cacgcatcgc acgttcgtca gaaccccctt ctgctttcac gcgatccatt
    34381 gtcagtttac ggaagatcac ggaaatcatc tggaaagtgg aaccactacc cagcccagct
    34441 gtcaggaaca gcgccaggaa gaccgcgaag aacgccatga agcttccgcc ctgcccgtca
    34501 gtcggtaagg tcaggaacag caggccgctg aaaatcgcca tcagaataaa gttcaccagc
    34561 gtgacacgag ttccgcccag acggtcagat aatgcaccac ctgcagaacg cgccagcgca
    34621 ccaataaacg gcccgaagaa agcgtattgc agaatctgaa catccgggaa ctgcgttttt
    34681 gacagcatcg caaagcccgc ggagaagccg atgaaggaac cgaaggttgc cagatacagc
    34741 aggctcataa tccacagatg accccgtttg agtaccggca actgctcctt gatggaggct
    34801 ttcgaggtag caagatcgtt catgccaaac cacgccgcaa tggtgaagat ggcaaggaac
    34861 ggcacccata tccaggacgc attcgccaga tacagctcag tcccatccgg ctgtttgacg
    34921 ccctggctac caaatactgc gaaaatcgac agtgatacca ccagcggagc aaccaactgc
    34981 ataacgctaa cgcccatgtt gcccagacca ccattcaggc ccagcgcgcc accctgcttc
    35041 tgtttcggaa agaagaagct gatgtttgcc atactggatg cgaagttcgc gccagcaaag
    35101 ccgcacagca gagagatgat gatgaagacg ctataaggcg tggaggtatc ctgcacggca
    35161 aaacccagcc agacacaagg aataatcaga ataccggtgc tgaacgccgt ccagcgacga
    35221 ccaccgaaga taggaaccat aaaggagtat ggaacacgta ataacgcgcc agaaaccgaa
    35281 ggcaacgcag tcaacataaa tagctgatcg gtcgtaaaat taaaaccgac tttcggtaag
    35341 ttcaccgcaa cagcgctgaa caacatccat acgcaaaacg ccagcagcag acagggaacg
    35401 gaaatccaca ggttgcggct ggcaatacgt tgaccgcgtt gttgccagaa cgcaggatct
    35461 tccggtcgcc aatctgtaat gacagctcca gtagcccttt cgggggcgga tgagtgactc
    35521 atagacacct ctgatactcg tttcgatgtc tgccacctta gtgtctgtag ctaaaggcaa
    35581 tttgatgtaa atcaaacgat aagataactt tatctatcat tgatatttat cattacccat
    35641 agtgagtaca gtgatttcat aaaaattatg agatttttca cggtgctgta aaatccctac
    35701 ccttaccgat gtaaagctac taaccacacg gcaaataagg agtaactctt tccgggtatg
    35761 ggtatacttc agccaatagc cgagaatact gccattccag aatgtatcgt cacattcatt
    35821 aaggttattg ctcatttaaa gcctgaagga agaggtttac atgcttaaac gttgtctctc
    35881 tccgctcacc ctggttaatc aggttgcgct tattgtgttg ctttctactg ctattggact
    35941 ggcagggatg gcggtttctg gctggctggt gcaaggcgtt cagggcagcg cccatgcgat
    36001 caacaaagcg ggatcgctgc gcatgcaaag ttaccgtctg ttggcagcag tgccattaag
    36061 cgagaaagac aagcccttaa ttaaagagat ggaacaaacg gcatttagcg ccgagttgac
    36121 tcgagcagca gaacgagacg gacaactggc gcaattacag ggtttacaag attactggcg
    36181 taatgaactc atccctgcac tgatgcgtgc acaaaaccgc gaaacggtgt cagcggatgt
    36241 cagccagttt gttgccgggc ttgatcagct ggtatctggt tttgaccgca ccacggaaat
    36301 gcgcattgag acagtggtac tggtccatcg ggtaatggcg gtatttatgg cacttttact
    36361 ggtgttcact attatctggt tgcgggcgcg actgctacaa ccgtggcggc aactgctggc
    36421 aatggcgagt gccgtcagcc atcgcgattt tacccaacgc gcaaacatca gcgggcgcaa
    36481 cgaaatggcg atgcttggaa ctgcgttgaa caatatgtct gcagaactgg ccgaaagtta
    36541 tgccgtactt gagcagcggg ttcaggagaa aaccgccggg ctggagcata aaaatcagat
    36601 cctctctttt ttatggcagg ctaaccgccg tttgcattcc cgcgccccgc tgtgtgaacg
    36661 cctgtcacct gtactcaacg gcttacagaa tttaaccctg ctacgtgata tcgaactgcg
    36721 ggtgtatgac actgatgatg aagagaatca tcaggagttt acctgccagc cagatatgac
    36781 ttgtgatgat aaaggctgcc agctctgccc gcgcggcgta ttacccgttg gtgatcgcgg
    36841 cacgaccctg aagtggcggc tggctgactc tcatacgcag tacggtattt tgctggcgac
    36901 cctaccgcag gggcgtcatc ttagccatga tcaacaacaa ctggtggata ccctggttga
    36961 acaactcacc gccacgctgg cgctggatcg gcatcaggaa cgtcagcaac agttgatcgt
    37021 gatggaagag cgtgccacca ttgcgcgcga actgcatgat tctattgccc aatctctctc
    37081 ttgcatgaag atgcaggtga gttgtttaca gatgcagggc gatgcgctgc cagaaagcag
    37141 ccgcgaactg ttaagtcaga tccgtaacga actgaatgca tcctgggcgc agttgcgtga
    37201 attgctcacc acattccgct tgcagctcac cgagcctgga ttacgtccgg cgctggaggc
    37261 gagttgcgaa gagtacagcg ccaaatttgg cttcccggtg aagctggatt atcaattgcc
    37321 gcctcgtctg gtgccttcgc atcaggcaat ccacttgttg caaattgccc gtgaggcatt
    37381 aagtaacgcc ctcaaacatt cgcaagcgag tgaggtcgtg gtgacggtgg cgcaaaacga
    37441 taatcaggtc aaactgaccg tccaggataa cggctgcggc gtgcctgaaa atgccatccg
    37501 cagcaatcac tacggcatga taataatgcg cgaccgtgcg caaagtttac gaggcgattg
    37561 ccgcgtccgc cgtcgtgaat caggtggcac cgaagtggtg gtcaccttta ttcccgaaaa
    37621 aactttcaca gacgtccaag gagataccca tgagtaatca ggaaccggct actatcctgc
    37681 tgattgacga tcacccgatg ctgcgaactg gcgtaaaaca gcttatcagt atggcaccag
    37741 atatcaccgt ggttggcgaa gcgagtaatg gcgaacaggg tattgaactg gcggaatctc
    37801 ttgatcccga tctgatcctg ttagatctca atatgcccgg catgaacggt ctggaaacgc
    37861 tggataaact gcgcgaaaag tccctctcag ggcgcattgt ggtattcagc gtctctaacc
    37921 atgaagaaga tgtggtcacc gcactgaaac gcggcgcgga tggctatctg ttaaaagata
    37981 tggaaccgga agatctgctg aaagcattgc atcaggcagc tgctggcgaa atggtattaa
    38041 gcgaagcatt aacgcctgtt ctggccgcca gcttgcgcgc taaccgtgcc acgactgagc
    38101 gcgatgttaa ccagttaacc ccacgcgagc gcgatattct caagctgatt gcccagggtt
    38161 tgccgaacaa gatgattgcc cgccgcctgg acatcaccga aagcacagta aaagtgcacg
    38221 tcaagcacat gctgaagaaa atgaagctca agtctcgcgt ggaagcagcg gtatgggtgc
    38281 atcaggagcg cattttctga ttacggttcc cagcgcagtt cgtcgtttga caatgcgtcg
    38341 aacggttcga caagcgttag cgtgatctca ttggaggaga cacgctgccc ctggttatct
    38401 tccaccacca ccgacaatcg ccagtgattg ctcgcccctt ccccgttctg ccagtcaggc
    38461 atgatcagcg tccagccctc cgcgctgttg gcttgtgcgc ccggcgtcaa acttaatatc
    38521 tgcgtatcgc cctgccaaat cagttgccga ataccgtaac gactgcggat ttgtaatttc
    38581 agcggcactg tttcgccagg ttttagatcc cacggcggtg tcgcaagaaa caccgttaac
    38641 gtttttcgct gtcggtactc aagagtcggt agattatttc gctgcggatt atcatagcga
    38701 ctaccacgta acgactgact ttcggcaacc tcgcccgcag aaagttgttt tttgagcggt
    38761 acaccaaagc ggtaattaag attcagcccg aggttatttt gattctcgcc actttcaccc
    38821 tgtttatgct gggccgtcac agtgactaat ggcacagggg tgtaatttaa tcccagactc
    38881 aacgcgacgg gattgtgata acccgtacca gagttaaaca aatcaacacg atcaccaaaa
    38941 tactgttcta cgctgacact ggtattgagg tgttgataga acggcatgcg catccgggct
    39001 gtcaggtcgt acccgcgcgc catccgttgt tcctgcgtgg ctgtctgttc atgccatgca
    39061 gcaaacggct gataaaagtt tgccgatagt cgcaaatatt cgccccacgc ttcggcacca
    39121 aagcccgctc gctgaagatt ttcatccagc aagttgtcat aaaaagtgtt ataacccacc
    39181 agccagttgc cgcgcgccca gcgttgccca acgcccacat tgctcaccag cccatcatcc
    39241 tgctgagtaa gaccaagctg gctccaggtg agataacgat cattatcttg taacggcaca
    39301 aaccagcttc cacgactgcc ggtaaaatga ccttcgttat ccactttgac gtcaacactg
    39361 gcatttcccc acggtgatag ccacgactct acgtgctgat taacctgttg actaagcgcg
    39421 tcgcggactt ttcccaatgc gaaagctttt gcctgctcgc cagtatccag cccgttatca
    39481 ttcatactgg tttcgccgaa atctttcaca atttcagcaa agtgtttttc cccatcatga
    39541 ttttcaggcg ccatgcctaa atccggcaga ccatcattgt tattatcaaa gggatttgcc
    39601 gctttttgct cgaaggtaga ttgtgcgtta gctgtaccgc ctgccgccag caagagtaaa
    39661 taaaacggaa taatgcgagg aacgaaacgg ctcaaaacgt cggtaacttc cggtaaaaaa
    39721 tgaaaaaaca tagccttaaa gaataacgat tactggcaac ttttgtcgta ttttctcgcg
    39781 ctttcaggaa aatccttaaa aatgtgaccg attggtcaga cgctgcagaa gcagtaagat
    39841 agaagatgag tgtctaattc cctgaataca ggagtaaata atgcaaaaaa tcgtgatcgt
    39901 tgccaatggc gcaccttacg ggagcgaatc cttgtttaac agcttgcggc tggccattgc
    39961 gttacgagag caggagagca atctggatct gcgtctgttc ctgatgtctg atgcggtcac
    40021 agccgggttg cgcgggcaaa aaccagggga aggctacaac atacagcaaa tgctggagat
    40081 ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc tgtaccgacg ggcgcgggat
    40141 tagtacactt cctctgattg atggggtgga aatcggtact cttgtggaac tggcgcaatg
    40201 gacgctgtca gccgataaag tgctcacatt ttaatgtttg agggggcgtc tgacgcccca
    40261 cgatgaggga agtttactca ggcgaatccc ggccgcaaca caccatccgg ataattctcc
    40321 gccagcagtt tttttaccga taccagcaaa tcattcagcc catcgtcctg cattcccagt
    40381 ttgataagct cctgttccag tgaaaacagg tattgtgcgt tggttcccag cggaccgctc
    40441 gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt cagattcata ttctggatgt
    40501 cgcgggtcca taataaacac aatggcgttt actgtgcgtc catcatcaag atcaagctga
    40561 caccaggttg gcagataaca gccggtaatc atctcgcgct tccacaacag ggttagttcc
    40621 tgctccagcg tctcttctgg cagtcgatag gcgacgcctg tggtacgtcc gccctctttt
    40681 agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc cggcggtcag gcgcaggcaa
    40741 aacgcgcgat gccatccaac cagtgtaccg gtgcacgact cggtaaactc cagtgccgga
    40801 ttccacatca acgatccata accgaagatc cacaccggtc tctcatcagg tcgacaagcc
    40861 agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc ataagagtga ttcttcaatg
    40921 gcaccaaatg ccgttttaca atcggcattc atcaagaaat cacgcgttat caccttctta
    40981 cacctccgcc actgcatgct tccctgcgac aactttttgg tgtcttccgg acatttttac
    41041 tgctcatttt gaaaccacta acgggacaat atgcaatccg cggaccactt gcaaggcaac
    41101 accgcacgaa tagctttaac taagctaccg gttttacgat tttttatgcc atttatcatc
    41161 atcccctttg gcatattcat gcttcacagc agcccaggcc actttatgcg cggtttcttc
    41221 gcgactggcg tcatcacgcc gatcttcttt atctttatat tgatcccatg cgctgttgaa
    41281 cgcttcttta tagatatcct gggcatgaga cggtagaacg tgctttacgc tttccggcag
    41341 gtcgcttttc gttttatacg gcatcgttga ctccttttac aaagttgaac attaagtgtg
    41401 gtcaacaata tctactcttg caaaaacata agaattttca tgtcagactg ttcttaagaa
    41461 acgctaaaac aaacattgcc taaacgtaat acttattttt atgccccaat gagtaaaaaa
    41521 tcgacaacgg ggtaaaaatt cgtaaaatgt taccgatatc cagacacttt ctgttatttt
    41581 tcctacctat tttttacatt ttcgataaat tcatctgcaa agggataacc ataatgtcaa
    41641 atgctcaaga ggcggtaaaa acccgccaca aggagacttc gcttattttc ccggttctgg
    41701 cgctggtagt gctgttcctg tggggaagca gccagacact accagtggtc attgccatca
    41761 atcttcttgc gcttattggt attttaagta gcgcctttag tgttgtccgt catgcggacg
    41821 tattagccca tcgcctggga gaaccttacg gttcgcttat tcttagcctt tcagtggtta
    41881 ttcttgaagt tagtttgatt tcagctttaa tggcaaccgg cgacgccgcg ccaacgctaa
    41941 tgcgtgatac gctctattcg atcattatga ttgttaccgg tgggctggtc ggcttttcat
    42001 tattgttggg cggtcgtaag tttgccaccc aatatatgaa tctgtttggt attaagcagt
    42061 atttaattgc gctgttcccc ctggcgataa tcgtactggt atttccaatg gctctgcctg
    42121 cggcgaattt ttcaaccggt caggcgttac tggtagcatt aatttctgcg gcaatgtatg
    42181 gtgtattttt gctgatccag accaaaacgc atcaaagttt gtttgtctac gagcacgaag
    42241 atgacagtga tgacgacgac ccgcaccacg gtaaaccgtc tgcccatagc agcctgtggc
    42301 atgctatctg gttgattatc cacctgattg ccgttattgc agtcaccaaa atgaacgcca
    42361 gcccgctgga gactttgctc gacagcatga atgcccctgt cgcctttact ggcttcctgg
    42421 tggcactgtt gattctgtcg ccggaaggtt taggtgcatt aaaagcagtg ttgaacaacc
    42481 aggtccagcg cgcgatgaat ctgttctttg gttcagtgtt agcaacaatt tcgcttaccg
    42541 tacctgtcgt cacgctaatt gcctttatga cgggtaacga attgcagttt gcacttggtg
    42601 cgccagaaat ggtggtgatg gtggcctctt tagtgctgtg ccatatctcc ttctccaccg
    42661 gacgtactaa cgtgctcaat ggcgcagcgc atctggcact gtttgccgcc tatttgatga
    42721 cgatatttgc ctgacagtta tatctcctcc gatgacggaa accgggggag ataatataaa
    42781 ttcctgctta ttgtatttct tcatactacc tggacaggtc gttaatcaga attgcgagcc
    42841 tgtaccacaa cctgcacgaa accactgcaa cagctgttga aaagccggaa tgtactcttt
    42901 attgcaaaca tttcagggat gaagtgaaga tgaattgaga cgtagtatcc acaccaagcc
    42961 ggcgttatcc ggtgaggcgc aatgttgcgg gggttttatc cctggtggca ttggttgctg
    43021 gaaagagaaa acccccgcac gttgagaggt aaaacctgac aacagggcta atcttgaaac
    43081 cagacacttc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct atatgacgct
    43141 cgcgcagttt gccatgactt tctggcacga cctggcggca ccgatcctgg cgggaattat
    43201 taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa ggtgaaaacg ggcgtcaggc
    43261 tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg taacagcctg cggccatttt
    43321 tgtctttatc acttccacaa gtaaatattg gcaacccgag ctggaagcat catccagagc
    43381 actcatgaaa cgactgtaac agctgttgaa aaaccggaat gcactcttca ttgcaaacat
    43441 ttcagggatg aagtgaagat gaattgagtc gtagtatcca caccaagccg gcgttatccg
    43501 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg aaagagaaaa
    43561 cccccgcacg ttgagaggta aaacctgaca acagaacggg ggctaatctt gaaccatcaa
    43621 caattcaaga atagccgcaa aatgttgtca ttacaacaag gcggctatat gacgctcgcg
    43681 cagtttgcca tgattttctg gcacgacctg gcagcaccga tcctggcggg aattattacc
    43741 gcagcgattg tcggctggtg gcgtaaccgg aagtaaggcg aaaacgggcg tcaggctgcc
    43801 gtgatggcaa tatgcgcccg aaaccggccg cagggtaaca gcctgcggcc ttttttgtat
    43861 ccggcaccca tgaaaaaagt cttaacgcag aacgctaaga ctttattttt caagcaaaaa
    43921 agattacttg ctggtatcca gttcttcgaa acctttcacc agatcatcaa tcgctttcat
    43981 ctgcctgagg aacggttcca gtttagccag cggcagcgcg gatggaccat cacatttcgc
    44041 atgttccgga tccggatgcg cttcaataaa cagccccgcc agacctaccg ccataccggc
    44101 tcgtgccagc tcagccacct gagcacgacg accgccggaa gctgcgccaa aaggatcgcg
    44161 gcattgcaat gcgtgggtca cgtcgaaaat caccggcgag ttaccagaca ctttcttcat
    44221 aatgctgaag cccagcatat cgacaaccag gttgtcatag ccgaagttag caccacgatc
    44281 gcaaagaatc actttttcgt tgccgccttc tttgaattta tcaacgatat tacccatctg
    44341 tcccgggctg acaaactgtg gtttcttgac gttaattacc gcaccggttt tcgccatggc
    44401 ttcaaccagg tcagtctggc gagcaagaaa cgccggcaac tgaatcacat ccacgacatc
    44461 agcaacgggc tgtgcctgac ttggttcgtg aacgtcggtg ataattttca cgccaaaagt
    44521 ctgcttcaac tcctggaaga ttttcatccc ttcttccagg cccggtccac gataagagtg
    44581 gatggaggag cggttggctt tgtcaaaaga ggctttgaac acgtaaggga tacccagttt
    44641 ctgggtcacg gttacgtagt gctcgcaaat gcgcatcgcc agatcgcgag attccagcac
    44701 gttcataccg ccaaacagta cgaacggcag gtcatttgct acgttgatgt cgccaatgct
    44761 aaccactttt tgtttcatag gatcgcctta accttgagta aaatgtcgat taattaatgc
    44821 agcacaatat gtttatgcgc gatgttattt atttgcgcac ggatcatttc gctgatcggg
    44881 tcttccggac actgttcaac gaaataactt aaatcgttca acgcaacgtg ttcgcaatcc
    44941 agttgcgcat aaatcaaccc gcgatcgcga atttcatagg gatcttccgg gttgaattgt
    45001 aataaagctt cgctggtgcg taacgccagc tccatctgat tttcttccat caacgaggct
    45061 ttgagtgtat ccagcaattt gcggattacc tcaatgttat cagcttcatc aaggtcttca
    45121 taaaacagtt ccgccgacgg gctgatattt ccctttaacc atacgtccag catatgttcg
    45181 cttaacgatt caccgttaaa aggattaatc agccaaattt cgccatccgg acattcaatg
    45241 cgcaatatca gctgcgtagg gaaaatcacc ggcagcagcg gcaaatcgag acgattcgcg
    45301 acccataata aaaccgcacc taatgatacc gcactgccct gtcgattctt taacacctgg
    45361 tccagccata atgcatcgga aagacgataa acgccgcgtg aggctttaaa tccccagtcg
    45421 ccgtaaaaca gcgcgatcag tttttccagc tgctcttcta aaggcagaag ctggctgatt
    45481 tcttccttcg ccagactaac gagacgctcc agttcgtcgt aaacatcttg cgagggaaaa
    45541 tcgcggcgga ttgcttcgca agccaggatc atgccttcgc acaatggcgc tttattaaat
    45601 tcgaaatcag ctaacgatct catgacttac cccagtaacg gtacttttgt ggtggcgagt
    45661 ttaatgatga tgtacagcac caccaacgtc agcgggaagg caataatgcg cgcctgctga
    45721 ctgtgcatac gacgataatc gagtgcaata aaacccaaaa cgatataaat gataactcca
    45781 aacagctttt cagtcagcca ctgtgcctgc ccggaaaatg gcaggatgtg cgctttagcc
    45841 atcaacgcaa tgccgcttaa caataacacc gtatcgacaa ccggcggcac aatgcgcgtc
    45901 cagcgagcaa atgcctgtgg atgcttctga taacgtagcc agaaacgtaa ggttaatagc
    45961 ccaacagaaa gcgcgatact aataagatga acactaagca gtgtagaaaa acttgtcatt
    46021 gataatagcg gccgagcgtt acgcgctcgt tatcaccata gtcacggcag gtttcgacgt
    46081 catgataccc cgcgaggata aatgcctgtc gcaccgcttc gccctgctgc cagccatgtt
    46141 ccagaagcag aaagccgccg gataccagcg cgttacgcga ctgttcgatg atatgcacga
    46201 tgtctgccat tccactgtct gccgcaacca gcgcagtgag cggctcaaag cggacatcgc
    46261 cttgttgaag atgagggtcc tgctcgtcaa tatacggcgg attgctgaca atcatcgcaa
    46321 actgctgccc ggctagcgcg ctaaaccagt cgctttgcag aatgtggata tttttgatcg
    46381 ccagatgctg ggcattacgt tgtgccaggg cgacagcatc gggcatacga tctacagcgg
    46441 taatttcgca gtccgggcgc tcgcgagcca gcgcaagcgc aatcgccccg gtacccgtcc
    46501 cgagatcgag aatacggcaa ggttgttcag gcaaccgcgc cagtgcctgc tccaccagac
    46561 actccgtatc cgggcgcgga attaaggtcg ctggcgaaac aaataacggc aacgaccaga
    46621 attctcgcac cccggttaaa tgagcaatgg gttcaccatc gcgacgacgt gtcagtagcg
    46681 catcaagttg ctgacattgt tcgtcagtca gctgcgtttc accaaaggcg agaataaaag
    46741 tacgccctct gccggtaaca tgctccagca ggatttcagc atcacgccgc gggctttcgc
    46801 tcgcctgaag ttggcttatt gcttcacgta accagtgttg atattccatt attcctgctc
    46861 ggacaacgcc gccagttggt cggcctgatg ttcctggata atcggttcaa tcagcatatc
    46921 cagcttacct tccatcactt catccaggcg gtagagcgtc aggttgatgc ggtgatcggt
    46981 aacgcgcccc tgcgggaagt tgtaagtacg gttacggtcg ctgcgatcgc cactccccag
    47041 caggttacga cgggtagacg cttcggcctg ttgccgtttt gccatttcag cagcgtggat
    47101 gcgagcaccg agtacagaaa gtgctttagc tttgttttta tgttgtgaac gttcgtcctg
    47161 acattcaaca acaatcccgg tcggcaagtg agtaatacga attgccgaat cggtggtgtt
    47221 aacgtgctga ccacccgccc ctgacgagcg gaaagtatca atgcgtaaat ctgctgggtt
    47281 gatgtccggc agttctgcgt caggcagttc tggcattacc gcaacggtac aagcagaagt
    47341 atgaatacga ccctgcgatt ccgtagcagg aacacgctgc acgcgatgac cgccagattc
    47401 aaatttcaga cgaccataca caccatcacc gctaattttg gcgatgatct ctttataacc
    47461 accatgttca ccctcgctgg cgctcatgat ttctacccgc cagcggcggg cttcggcgta
    47521 acggctgtac atacggaaca gatcgcctgc gaacagcgct gcttcgtcgc cgccggttcc
    47581 ggctcggact tcgaggaagg cgttacgttc gtcatcagga tcttttggca gtaacagaac
    47641 ctgtaattgc tgttccagtt gctcgctttt ttctttagct tcgcgcagtt catcctgcgc
    47701 catctcacgc atttcaggat catcgagcat catctgtgcg gtttcgatat cttcctgaac
    47761 ctgttgccag tcggtaaaac agcgcgaaac atcacttaac tgcgcatatt cgcgtgataa
    47821 tgcgcgaaaa cgttcctggt cggcgatagt ttgcgcgtca cccagcaacg cctgaacttc
    47881 ttcatggcgt tcatgcaggg cttccagttt ggcaacgata gaaggcttca taggcgtaaa
    47941 tgcaccctgt aaaaaaagaa aatgatgtac tgctactcca gcccgaggct gtcgcgcaga
    48001 atattcaggc gttcgttatc cccgtcacgg gcggcctgtt gaagtgattt cgttggcgca
    48061 tggatcaagc ggttagtcag tttccatgcc agatcctgca taatggcttg cgcgtcgccg
    48121 ccctgctcaa gggccgctaa cgctttggcg gttaactcat cgcgaacttg ctctgcctgg
    48181 ctgcgatact cgcgaatggt ttcgctggcg ctttgtgctc gcagccacgc cataaattcg
    48241 ctggtttcct gagcgacaat agtttccgcc tcaaccgctg cggctttacg ctgcgccagg
    48301 ttgtgcgaaa tgatgctttg cagatcatca acgctataaa gataagcatt cgccagtttg
    48361 ccaacttccg gctcaacatc gcgcggaacg gcaatatcca ccaacagcat tggttggttg
    48421 cgacggcttt ttaatgcgcg ctccaccatg cctttcccga taatcggtaa cgggctggcg
    48481 gtggaactga tgatgatatc ggcttcgcgc agacgttcgt cgatatcact cagggcaatc
    48541 acttccgcgc cgacttcatc tgccagaatt tgggcacgtt cgcgagtgcg gttggcgata
    48601 atcatcttct gtactttatg ttcgcgcaga tgacgcgcta ccagctcgat ggtttcgccc
    48661 gcgcctacca gcaacactgt gaccgtagag agcgattcaa agatctgccg cgccagcgta
    48721 caagccgcaa aagcgacaga cacagcgctg gcaccgatat ctgtttcagt gcgtacgcgt
    48781 ttcgctacag agaaagattt ctggaacatg cgttccagtt cgctggcctt catatgacct
    48841 ttttgcgaat cggcaaacgc ttttttaacc tgaccgagga tctgcggctc cccaagaacc
    48901 agtgaatcca ggccgctggc aacacgcatt aaatggctaa ccgcgtcgtt atcctgatgc
    48961 cagtagaggc ttttacgcag atcttcttca ttaagattgt gataatcgca aagccagcgg
    49021 attaacgcct cttgcaagtt gtcctgctct tcgacgctaa gataaagctc agtgcggtta
    49081 cacgtcgaca gcaccacccc gccctgcacc atcggctgtg cgagcagact gtccagcgcc
    49141 tgatcaagtt tgtccggcga aaacgataca cgttctcgca gcgatacagg tgccgtttta
    49201 tggttaatac cgagcgctaa aagggtcatg tctgcgggaa ataataccaa cgttgataag
    49261 gttagtctcg gcgcatcata ccgtatgcgc gagatcaata aaagggagcg cccccttttg
    49321 gagtaattgc ccgaacccgt tagatttcta caattaagac aacttgaaca tagacgatga
    49381 ctaatggtga cgctagcatt aagggttata actgcaacgt atctcaagga cttgtcatca
    49441 ctatgcccct gcccgatttt cgtcttatcc gcctgctacc gctggctgct cttgtgctca
    49501 ctgcctgttc cgttaccacg cccaaaggtc ctggcaaaag cccggattcg ccacaatggc
    49561 gtcagcatca gcaagacgtg cgcaatctta atcagtatca gactcgcggc gcgttcgctt
    49621 atatttctga ccaacaaaaa gtgtacgccc gctttttctg gcagcaaacc ggccaggatc
    49681 gctaccgtct gctgctcact aacccattgg gcagcacgga actggagctg aatgctcaac
    49741 cgggtaacgt gcagttagtc gacaataaag gtcagcgtta taccgccgat gacgccgaag
    49801 agatgattgg caaattgact ggaatgccaa ttccgctcaa cagcttgcgc cagtggattt
    49861 taggtttacc gggtgatgca accgactaca aactggacga ccagtaccgc ctgagcgaaa
    49921 ttacctacag ccagaatggc aaaaactgga aggttgttta tggtggttat gacaccaaaa
    49981 cgcaacctgc gatgccagcc aatatggaac tcaccgacgg tggtcaacgc atcaagttaa
    50041 aaatggataa ctggatagtg aaataatgcg gacacagtgg ccctctccgg caaaacttaa
    50101 tctgttttta tacattaccg gtcagcgtgc ggatggttac cacacgctgc aaacgctgtt
    50161 tcagtttctt gattacggcg acaccatcag cattgagctt cgtgacgatg gggatatccg
    50221 tctgttaacg cccgttgaag gcgtggaaca tgaagataac ctgatcgttc gcgcagcgcg
    50281 gttgttgatg aaaactgcgg cagacagcgg gcgtcttccg acgggaagcg gtgcgaatat
    50341 cagcattgac aagcgtttgc cgatgggcgg cggtctcggc ggtggttcat ccaatgccgc
    50401 gacggtcctg gtggcattaa atcatctctg gcaatgcggg ctaagcatgg atgagctggc
    50461 ggaaatgggg ctgacgctgg gcgcagatgt tcctgtcttt gttcgggggc atgccgcgtt
    50521 tgccgaaggc gttggtgaaa tactaacccc ggtggatccg ccagagaagt ggtatctggt
    50581 ggcgcaccct ggtgtaagta ttccgactcc ggtgattttt aaagatcctg aactcccgcg
    50641 caatacgcca aaaaggtcaa tagaaacgtt gctaaaatgt gaattcagca atgattgcga
    50701 ggttatcgca agaaaacgtt ttcgcgaggt tgatgcggtg ctttcctggc tgttagaata
    50761 cgccccgtcg cgcctgactg ggacaggggc ctgtgtcttt gctgaatttg atacagagtc
    50821 tgaagcccgc caggtgctag agcaagcccc ggaatggctc aatggctttg tggcgaaagg
    50881 cgctaatctt tccccattgc acagagccat gctttaagcc gggcaagctg agtttcggtg
    50941 acaacgtcac cttgttccag acgttgcatc gcgctcttta atacaccgcc tggaaaggat
    51001 catgcctggc ccgcacagtt ttcggcagat tctttccacc aatggacgca tgcctgaggt
    51061 tcttctcgtg cctgatatga agctttttgc tggtaacgcc accccggaac tagcacaacg
    51121 tattgccaac cgcctgtaca cttcactcgg cgacgccgct gtaggtcgct ttagcgatgg
    51181 cgaagtcagc gtacaaatta atgaaaatgt acgcggtggt gatattttca tcatccagtc
    51241 cacttgtgcc cctactaacg acaacctgat ggaattagtc gttatggttg atgccctgcg
    51301 tcgtgcttcc gcaggtcgta tcaccgctgt tatcccctac tttggctatg cgcgccagga
    51361 ccgtcgcgtc cgttccgctc gtgtaccaat cactgcgaaa gtggttgcag acttcctctc
    51421 cagcgtcggt gttgaccgtg tgctgacagt ggatctgcac gctgaacaga ttcagggttt
    51481 cttcgacgtt ccggttgata acgtatttgg tagcccgatc ctgctggaag acatgctgca
    51541 gctgaatctg gataacccaa ttgtggtttc tccggacatc ggcggcgttg tgcgtgcccg
    51601 cgctatcgct aagctgctga acgataccga tatggcaatc atcgacaaac gtcgtccgcg
    51661 tgcgaacgtt tcccaggtga tgcatatcat cggtgacgtt gcaggtcgtg actgcgtact
    51721 ggtcgatgat atgatcgaca ctggcggtac gctgtgtaaa gctgctgaag ctctgaaaga
    51781 acgtggtgct aaacgtgtat ttgcgtacgc gactcacccg atcttctctg gcaacgcggc
    51841 gaacaacctg cgtaactctg taattgatga agtcgttgtc tgcgatacca ttccgctgag
    51901 cgatgaaatc aaatcactgc cgaacgtgcg tactctgacc ctgtcaggta tgctggccga
    51961 agcgattcgt cgtatcagca acgaagaatc gatctctgcc atgttcgaac actaatcgaa
    52021 cccggctcaa agacccgctg cggcgggttt ttttgtctgt aatatccatt tgtatgacct
    52081 atgcctcctt cacctgccat ttagttgaca gatgatgcgc tcacggatga aacattattg
    52141 tgaacaaaat attttcctca catgtgatgc ctttccgcgc tctgatcgac gcttgctgga
    52201 aagaaaaata tactgccgca cggtttaccc gtgacctgat tgccgggata accgtcggga
    52261 ttattgctat cccgctggcg atggcgttgg ctattggtag tggtgtggca ccccagtacg
    52321 gtttatatac cgcagctgtt gcggggattg tcattgctct gacgggtggg tcacgcttta
    52381 gcgtttccgg tccgactgcg gcatttgtgg taattctcta tcccgtttcg caacagtttg
    52441 ggctggcagg actgctggtt gcgaccttgc tgtcggggat ctttttgatt ctgatgggtc
    52501 tggcacgctt tggtcgcctg attgagtata ttccggtttc cgtcacctta ggtttcacct
    52561 cgggtatcgg gatcaccatc ggtaccatgc agattaaaga ttttctcggt ctgcaaatgg
    52621 cccatgtccc ggaacattat ctacaaaaag tcggcgcatt atttatggcg ctgccgacca
    52681 ttaatgtggg tgatgctgcc attggcattg tgacgctagg tattcttgtt ttctggccgc
    52741 gtctgggcat tcgtttaccc ggtcaccttc cggccttgct ggctggttgc gcggtgatgg
    52801 ggattgttaa cctgctcggc ggacatgttg ctaccatcgg ttcgcaattc cactacgtcc
    52861 tggccgatgg ttctcagggt aacggtattc cgcaactgct accgcaactg gtgctgccgt
    52921 gggatctgcc taattcagaa ttcacgctaa cctgggattc tattcgcaca ctgctgcctg
    52981 cggcattctc aatggcaatg ctcggcgcaa tcgaatctct gctctgcgcc gtggtgctgg
    53041 atggtatgac cgggacgaag cacaaggcga atagcgaact ggttggacag gggctgggga
    53101 atatcatcgc tccgttcttt ggtggtatta ccgctaccgc tgccatcgcg cgttctgccg
    53161 ctaacgtccg tgccggggca acttccccta tctcggcggt gatccactct attctggtta
    53221 ttcttgccct gctggtactg gcaccgctgc tctcctggct gccgctttcc gctatggcag
    53281 ccctgctgtt gatggtggcg tggaacatga gtgaagcgca taaagtggtc gacttgctgc
    53341 gtcatgcacc gaaagatgac atcattgtca tgctgctgtg catgtcgctg accgtgctgt
    53401 ttgatatggt tattgccatc agcgtgggga tcgtgctggc atcgctgctg tttatgcgtc
    53461 gtatcgcacg tatgactcgc ctggcaccgg tagtcgtaga tgttccagac gatgtcctgg
    53521 ttctgcgcgt tattggcccg ctgttttttg ctgctgctga aggcttattc acggacctgg
    53581 agtcacgtct tgaaggcaaa cggattgtga ttctgaagtg ggatgccgtt ccggtacttg
    53641 atgctggtgg tcttgatgcg ttccagcgtt ttgtgaagcg tctgcccgaa ggatgtgaac
    53701 tgcgcgtgtg caacgtggaa ttccagccac tgcgcactat ggctcgcgca ggcattcaac
    53761 cgatcccggg acgcctcgcg ttcttcccga atcgtcgcgc ggcgatggcg gatttataag
    53821 tacgagattg accagtcagc gcaaactgac tggtcagcaa actgcattat ttgttagcta
    53881 tgacggcgat tatcgctacg gcggcaacgt ttgtcatagt ggcgaaccca ccagtaacgg
    53941 tcgcacacct gttcatgccc gccaacacgg gcacccgcca gccagagaat ggcaccgacg
    54001 aaaatactta gcactgcacc atgtgcgaaa tactggggca tattaaactg tggtaactgg
    54061 ttgaggattg aataccccac gccgaccacc attaccacca gacccaaccc catgagcacg
    54121 ttaccgagta acgaagcgtt tttgcgtttc atatgtcacc tccggaactt tctgggttgt
    54181 aacagggaat acccctcttc cttatgtgta aagtatagac aacaccctgt gggttatgtg
    54241 cgggcgtgat cacaattaca acccttattt caacaaaact ttacaaataa acgcctgaac
    54301 accttgactt tctaatagtc cactcaggca attattacgt tatctgcatt tgatacgcag
    54361 ttttttgtca agcggggccg caaccagtaa actacgcgcc agttatgtac acactcagga
    54421 caaaaaaacg tgacgattaa attgattgtc ggcctggcga accccggtgc tgaatacgcc
    54481 gcaacgcgac ataatgctgg tgcctggttc gttgacttac tggcagagcg tttgcgcgct
    54541 ccgctgcgcg aagaggctaa attctttggt tatacttcgc gagtcaccct tggaggcgaa
    54601 gatgtccgcc tgttagtccc gactacattt atgaatctca gcggcaaagc cgttgcggcg
    54661 atggccagtt ttttccgcat taatccggac gaaattctgg tggcccacga cgaactggat
    54721 ctgcctcctg gcgtcgccaa atttaagttg ggcggtggcc atggtggtca caatggactg
    54781 aaagacatca tcagtaaatt gggtaataac cctaactttc accgtttacg catcggaatc
    54841 ggtcatccgg gcgataaaaa caaagttgtt ggttttgtgt taggcaaacc gcctgttagt
    54901 gaacagaagt taattgatga agccatcgac gaagcggcgc gttgtactga aatgtggttt
    54961 acagatggct tgaccaaagc aacgaaccga ttgcacgcct ttaaagcgca ataagtcgtt
    55021 gtctgcggca tttttgccga gtgccgtgta taataggcaa agttatttcc atttctgcaa
    55081 tctgttagca ataacaggtt gattattaag atattaaggt gatttaaatc atgggattca
    55141 aatgcggtat cgtcggtttg cccaacgtcg ggaaatctac cctgttcaac gcgctgacca
    55201 aagccggtat tgaagcggcc aactttccat tctgcaccat tgagccgaac acaggcgtcg
    55261 taccaatgcc tgatcctcgc ctggatcaac tggctgaaat cgtaaaaccg caacgtacgc
    55321 ttcccacgac catggaattt gtcgatatcg ccggtctggt aaaaggcgca tcgaaaggcg
    55381 aaggtctggg taaccagttc ctgaccaaca tccgtgaaac cgaagcgatc ggtcacgttg
    55441 ttcgctgctt tgaaaatgac aacatcattc acgtttccgg caaagttaac ccggctgacg
    55501 atattgaagt tatcaacacc gaactggcgc tggcagacct cgacacctgc gaacgtgcga
    55561 ttcatcgcgt acagaagaaa gccaaaggtg gcgataaaga cgcgaaagct gagctggcgg
    55621 tcctggaaaa atgcctgccc cagctggaaa acgcaggtat gctgcgcgcg ctggatttaa
    55681 gcgctgaaga gaaagcggct attcgttacc tgagcttcct gacgctgaaa ccaacaatgt
    55741 acatcgccaa cgtcaacgaa gacggttttg aaaacaaccc atatcttgac caggtgcgtg
    55801 aaatcgcggc gaaagaaggt tctgttgtgg ttccggtttg tgctgctgtt gaagcagaca
    55861 ttgccgaact ggacgacgaa gaacgtgacg agtttatgca ggagcttggg ctggaagagc
    55921 cgggtctgaa ccgtgtgatc cgtgccggtt ataagctgct gaacctgcaa acttacttca
    55981 ccgctggggt gaaagaagtg cgtgcatgga ccattccggt tggagcaacc gcgccgcagg
    56041 cagcgggcaa aatccatact gattttgaaa aaggctttat ccgtgcacaa accatctcgt
    56101 ttgaagattt catcacttac aaaggtgaac aaggcgcgaa agaagcaggc aaaatgcgtg
    56161 cagaaggtaa agattacatc gtgaaagatg gcgatgtgat gaacttcctt ttcaacgtct
    56221 aattaattta ttgtctcatg aggtttcgct aaatctcatg aagagcgcaa aagtatataa
    56281 aatccacgca attgcgtgga tttttcattt caccgagtcc taatcattct tgtaacatcc
    56341 tacacttttg tgcttattgc ctacagtgaa ccacgagaat aattaaagac atcagtgttc
    56401 agatgacgga tatctcatta gcagtgatat aacgtcagtt tttgttccac tacacatcag
    56461 cccccttcac agccgatcaa tattgaccac gctccaatat gctggcgaga aaaggactta
    56521 cctgaagggt aatatcccgc agataaagtc cccgaataac gtgattacgt gcgatcctgg
    56581 caagatgaaa atgataacca gtagcacaat aacaacgata gaccagaaaa cccattgacc
    56641 aaaaatgaat atcgatgttt tttgcttttt cttattagta atgcaaaaat ggcgcatatt
    56701 gtttttgtta tgcagataca tcaagcgaaa atcccataac taattaggat tattaccaaa
    56761 aatattcacc aaattattta accgctgttt ggtgtaatgt ttttgtcata ttaatagtaa
    56821 cgaattggaa taagtaacga caacttaggg aataactcga tctaaactca cctttgtaag
    56881 ggggatgtta aatatatatt aatttctgtg tttctgattt tgcccggtaa tagagaatta
    56941 ttctcagaga agctcatcat gattgcacca tacaaatatc atgatgataa gaggatatta
    57001 ttcattgact taaacaaaat gacaaaatcc atagacccat agcctgggta ctatatagga
    57061 gtggtatggg catcaaacaa cacaatggga ataccaaagc cgatcgtctc gctgaattaa
    57121 aaatccgttc gccctcaatt caactgataa aatttggcgc tattggtttg aatgcaattc
    57181 tcttttcccc cctgctgata gctgctgata caggaagtca atatggcacc aatattacta
    57241 ttaatgatgg tgacagaatt actggagata ccgccgatcc atcaggaaac ctctatggtg
    57301 taatgacccc agcaggaaac acgcctggca atatcaacct gggtaatgat gtcaccgtca
    57361 atgtcaacga cgcctctgga tatgcaaaag gaatcattat tcagggcaaa aacagctccc
    57421 tgacagctaa ccgactcaca gtagatgttg ttggtcaaac ctctgccatc ggcattaatt
    57481 taattggtga ctatacccat gctgacttag gcacaggcag caccattaag agtaacgatg
    57541 acggcatcat tattgggcat agctcaacac taacagccac tcaattcacc attgaaaact
    57601 cgaacggtat aggcctaacc atcaatgact atggcaccag tgtcgatctt ggaagcggaa
    57661 gtaaaatcaa gaccgatgga agtacaggtg tttatatcgg tggtctcaac ggcaataacg
    57721 ccaatggtgc tgcgcgtttt acggcgacag acctgacaat cgatgttcag ggctacagcg
    57781 ccatggggat aaacgtacag aaaaactctg ttgtcgatct cggaacaaac agttccatta
    57841 aaaccagtgg cgataatgca cacggcctct ggagctttgg ccaggttagc gcgaatgcac
    57901 tcactgttga tgtaactgga gccgcggcca atggcgtcga agttcgtggt ggtacaacca
    57961 ctatcggtgc agatagccat atttcttccg cgcagggcgg tggtctcgtc accagtggtt
    58021 cagacgcgac aatcaatttt tctggcacgg cagcgcaacg aaacagcatc ttttccggcg
    58081 gttcttatgg tgcctcggcc cagacggcaa cggctgttat caacatgcaa aataccgata
    58141 ttacggttga tcgtaatggc agtctggcgc tgggtttgtg ggcgctcagc ggcggtagaa
    58201 taaccggaga cagtttggct atcaccggcg cggcaggagc cagggggatt tatgccatga
    58261 ccaacagcca gatcgacctc acgagcgatc tggtcattga tatgagtaca cccgaccaga
    58321 tggccatcgc aacgcaacat gacgatggtt atgccgccag ccgcatcaac gcctcgggtc
    58381 gtatgcttat caacggtagc gttctttcca aaggtgggct aatcaatctg gatatgcacc
    58441 ctgggtcggt ttggacaggt tcctccctca gcgataatgt caatggcggg aaactggacg
    58501 ttgcaatgaa taacagcgtc tggaacgtaa caagtaattc taatctcgac acgctggcgc
    58561 tgagccattc aactgtcgat tttgccagcc acgggtcaac tgccggcaca tttaccacat
    58621 taaacgtaga gaacctgagc ggtaacagta cctttattat gcgtgctgat gttgttggcg
    58681 agggtaatgg cgttaataat aaaggggatt tattgaatat cagcgggagt agtgctggta
    58741 atcacgtatt ggctatccgc aaccagggca gcgaggccac aacgggaaat gaagttctga
    58801 cagtggtaaa aaccactgac ggtgcggcct cgttcagcgc gtcttctcag gttgagttgg
    58861 ggggatatct gtacgatgtg cgtaaaaatg gcactaactg ggagctttac gcttccggga
    58921 cagttccgga accgactcct aatcctgaac caacaccagc tcccgctcag cctcccatag
    58981 tcaaccccga tcctacgcct gaacccgatc ctacgcctaa ccccactccc acacctaaac
    59041 ccaccacgac cgcagatgct ggcggcaatt atctcaatgt cggttactta ttaaactatg
    59101 ttgaaaaccg tacgctgatg caacgaatgg gtgacctgcg aaatcagagt aaagacggta
    59161 atatctggct gcgcagttat gggggaagcc tggactcctt cgccagtggc aaactgagcg
    59221 gctttgacat gggttacagc ggtatccagt ttggtgggga taaacgtctc tctgatgtaa
    59281 tgccgttgta tgtcggtctg tatattggct caacacatgc atcgccggac tatagcggtg
    59341 gcgacggtac cgcacgttca gactacatgg gaatgtacgc cagttacatg gcacacaacg
    59401 gtttttacag cgatctcgtt gtaaaagcat cgcgccagaa aaatagtttc cacgtactgg
    59461 acagtcagaa caacggcgtt aacgccaacg gcactgcgaa tggattgagc atctccctgg
    59521 aagccgggca gaggttcaac ctgaccccta ctagttatgg gttctatata gagccgcaaa
    59581 cccagctcac atacagccac cagaatgaga tggctatgaa ggcgagtaat ggtctcaata
    59641 tacatctgaa tcactacgaa tcgctgctgg ggcgtgccag catgatactg ggatatgaca
    59701 tcaccgcagg caacagccag ctgaatatct atgtgaagac tggcgctatc cgcgagtttt
    59761 caggggatac agaatatcag ttgaataact cccgggagaa gtacagtttc aaaggtaatg
    59821 gctggaataa cggcgtggga gtcagtgcac agtataacaa acagcacaca ttctacctcg
    59881 aagcggatta cacgcagggt aacctctttg atcagaagca agtcaacgga ggatatcact
    59941 tcagctttta agtacggggt agacaagccc ttaaacgatg ctgaaaaatg ttagcgtgcg
    60001 ccggaatgaa atatagatca ctctgatgta tcagaaatcg tctctgtctt tcatacccgg
    60061 cgcttaaact gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg
    60121 ccgattccaa gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta
    60181 ataattgcct ctttttcaac ttccgcaaat gacagactgg tggaaaggcg tgtggcgctg
    60241 acatcatctg tcgcctgctc ggtaaacaga tgttccggca aatcactgac gcgaatgcgc
    60301 ccattatcac tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcca
    60361 ggccatgcac aggaaaccag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag
    60421 aagcgttttt caagactgcg taatttgtta ttcaccagag ccggaatgct gccacgccgc
    60481 atacgcagag gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta
    60541 aaacgatttt gttccaccag cattgcgagg tccgcggtcg ttgtagcaat cactttgaca
    60601 tcaattggta ttaaacgccg cgcatccagt cgcgtgataa caccctgctt gataacctga
    60661 agcaaagcag actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg
    60721 ccgtgcgcca gttccagccg actcagacgg ccattttcat tgtccgtgcg atcgccacca
    60781 ataaattctt ccgccagtgc cgcatcacca tataactcac aattgacggc gatataagga
    60841 cctgcagcac gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc
    60901 tcttctccac aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc
    60961 aagcggcggg tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct
    61021 aattgactgg tcattaactg ccgcatctgt tccacaggat ggagcaacaa aataaagctg
    61081 gttccctgcg tttctattat cggtttaagg gttatcaccg catcaataaa ctggtgctgg
    61141 ctttcaaagg ttgcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat
    61201 acggcgggta acgttaagag ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg
    61261 cgcaagaccc gcgccgcctg ggcattaata aattgcaaat taccctgctc gtcccagcta
    61321 atcacgccat catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt
    61381 tcagcgagca aactgtccgt cagcagtaaa tttccgacct cgcgggcgat tgccaacgtc
    61441 aacggcaaat cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc
    61501 aatcggccct tgctgtcaaa taacggcgtt acacaaaagg cccagttcca gagtgcctgt
    61561 ttgaaatgtt gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc
    61621 gcacaagtac caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt
    61681 agctgctgca aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg
    61741 ataaacagcg cacactctcg cggagccata tattcccagg catcttccag cgcagcctgc
    61801 ccgagcgtca gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc
    61861 tgatgtggta cgttccatgt ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt
    61921 gcaattaagg gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc
    61981 ccttatgaca aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca
    62041 atatggaaca ttcattaaag catatgtttc actatgaaac ataatttggg ctaattttct
    62101 tttcccttgc ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac
    62161 atcatccatg ccctaccgta attgctggag caaaataatg aaaaaattga tcaatgatgt
    62221 gcaagacgta ctggacgaac aactggcagg actggcgaaa gcgcatccat cgctgacact
    62281 gcatcaggat ccggtgtatg tcacccgagc tgatgcccct gttgcaggaa aagtcgccct
    62341 gctgtcgggt ggcggcagcg gacacgagcc gatgcactgt ggttatatcg gtcaggggat
    62401 gctttcgggg gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga
    62461 atgcgccatg caagttgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg
    62521 cgatattctt aactttgaaa cagcgaccga gttactgcac gatagcggcg taaaagtgac
    62581 cactgtggtc attgatgacg acgttgcagt aaaagacagt ctttataccg ccgggcggcg
    62641 cggcgttgcc aacaccgtat taattgaaaa actcgtaggc gcagcggcgg agcgtggcga
    62701 ctcactggac gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg
    62761 tatcgctctc ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta ccctggcgga
    62821 taatgagatg gagtttggcg tcggcattca tggtgagccg ggtattgacc gtcgcccctt
    62881 ctcttccctt gatcaaaccg tcgatgaaat gttcgacacc ctgctggaaa atggctcata
    62941 ccatcgcact ttgcgtttct gggattatca acaaggcagt tggcaggaag aaccacaaac
    63001 caaacaaccg ctccagtctg gcgacggggt gattgcgctg gttaacaatc ttggcgcaac
    63061 tccactttct gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg
    63121 attgactatc gagcgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt
    63181 ctcaatcact ttactgaaag ttgatgacga aacgctggca ctctgggacg ccccggtcca
    63241 caccccggcc cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa
    63301 ttgttaactg gctcactcgt tgtggcgata ttttcaacac cgagagcgag tatcttaccg
    63361 gactggatcg cgagattggc gacgctgacc acgggctaaa tatgaaccga ggctttagca
    63421 aagtggtgga aaaactccct gctattgcag ataaagatat cggtttcatt ctcaagaata
    63481 ccggtatgac gctgctttcc tgcgtcggtg gtgccagtgg tccgctgttc ggtaccttct
    63541 ttatccgcgc cgcacaggcg acccaggcac ggcaaagcct gacgctggaa gagctttatc
    63601 agatgttccg cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata
    63661 aaaccatgtg tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa
    63721 atctctctgt tccggcggcg ctcgaagctg ccagtagcat cgccgaattc gctgcacaaa
    63781 gtacgattac gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg
    63841 gtcaccagga tcccggcgca acctcggtga tgtttatgat gcaaatgttg gcgttagccg
    63901 caaaagagta aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg
    63961 ggagaaggtg tcggtgaatt agcccgtcag atgttaatga gtgataattg taaaatcgcc
    64021 attgctgcgg gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg
    64081 gaggccatcg aatctgttgc tgatgccgac catgtgctgg tcatgatgga tatgggtagc
    64141 gcattattga gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta
    64201 cgtttgtgtg ctgcgccgtt ggtcgaaggt acactggcag caactgtcag cgcggcctcg
    64261 ggggcggaga tcgacaaagt tatctttgac gccatgcatg cgctggaagc caaacgtgaa
    64321 caactgggtt taccgtcctc cgacactgaa atctctgact catgtcctgc gtacgatgaa
    64381 gaagcccgtt ctctggcggt ggtcattaaa aaccgtaacg gcctgcacgt ccgtccggcc
    64441 tcccggctgg tttatacctt atcgacattt aatgccgata tgttgctgga aaaaaacggc
    64501 aaatgcgtca caccggagag tattaaccag attgcgttac tacaagttcg ctataacgat
    64561 acgctgcgcc tgattgcgaa agggccagaa gctgaagagg cactgatcgc tttccgtcag
    64621 ctggctgaag ataactttgg tgaaacggag gaagttgctc cacctactct gcgtcccgtt
    64681 ccgcctgttt cgggtaaagc cttttattat caaccagttt tatgtacggt acaggcaaaa
    64741 tcacccctga ccgtggaaga agaacaggaa cgattacgcc aggccattga cttcacgtta
    64801 ttagatctga tgacgttaac agcaaaagca gaagccagcg ggcttgacga tattgccgca
    64861 atcttttctg gtcaccatac actgttagat gatccggaac tgctggcggc ggcaagcgaa
    64921 ctccttcagc acgaacattg cacggcagaa tatgcctggc agcaagttct taaagaactt
    64981 agccagcaat accagcaact ggatgatgaa tatctacaag ctcgctatat tgatgtggac
    65041 gatcttctgc atcgcaccct ggtccacctg acccaaacga aagaagaact cccgcagttt
    65101 aactcgccaa ctattctact ggcggagaac atttatccct ccccagtact gcaactggat
    65161 ccggcggttg taaaaggtat ctgccttagc gccggaagtc cggtatccca cagcgccctg
    65221 atcgcccgtg aactggggat tggctggatt tgccagcagg gtgagaaact gtatgcgata
    65281 caaccagaag aaaagctaac gctggacgtt aaaacgcaac gtttcaaccg tcagggttaa
    65341 gccaccgttc ggatggcatc gttctgatgt catccggcaa tttactcagc actaatgttt
    65401 tttaactacc tgttatattt ttcaccaatt tatgatgatt tttcttcgct aatcgcgaac
    65461 caaacgacgc tggctggcta ataaacagac gttatttgat cactctattt cgccgttatt
    65521 tatcccttta attccctttt ctaaaatgcc tgacagttcg cagaatgaga tttcgatcat
    65581 gcagctagtg cgatcctgaa ctaaggtttt ctgatacttg aataccgttt tattcggttt
    65641 cgcctaagga gaatgattga tgaaatcccc cgcaccttct cgcccgcaaa aaatggcgtt
    65701 aattccagcc tgcatctttt tgtgtttcgc tgcgctatcg gtgcaggcag aagaaacacc
    65761 ggtaacgcca cagccgcctg atattttatt agggccgctg tttaatgatg tgcaaaacgc
    65821 caaacttttt ccggaccaaa aaacctttgc cgatgccgtg ccgaacagcg atccgctgat
    65881 gatcggatgc agcaaaacca gagcggattt gatctgcgcc atttcgttaa cgtcaatttc
    65941 accctgccga aagagggcga gaaatatgtt ccgccagagg gacagtcact gcgcgaacat
    66001 attgacggac tttggccggt attaacgcgt tctaccgaaa acaccgaaaa atgggattct
    66061 ctgttaccgc tgccggaacc ttatgtcgtg ccaggcggac gttttcgcga ggtatattac
    66121 tgggacagtt acttcaccat gttaggactt gccgaaagcg gtcactggga taaagtcgcg
    66181 gatatggtgg ccaattttgc tcatgaaata gacacttacg gtcatattcc caacggcaac
    66241 cgcagttact atttaagccg ctcgcagccg cccttctttg ccctgatggt agagttactg
    66301 gcgcagcatg aaggcgatgc cgcattgaag caatacctgc cgcaaatgca aaaagaatat
    66361 gcttactgga tggacggtgt tgaaaacctg caagccggac aacaggaaaa acgcgttgta
    66421 aaacttcagg atggtaccct tctcaaccgc tactgggacg atcgcgatac gccacgacca
    66481 gagtcatggg tggaagatat tgccaccgct aaaagcaatc cgaatcgacc tgccactgaa
    66541 atttaccgcg acctgcgctc tgctgctgcg tctggctggg atttcagctc gcgctggatg
    66601 gacaatccgc agcaactaaa taccttacgc accaccagca tcgtaccggt cgatctgaac
    66661 agcctgatgt tcaaaatgga aaaaatcctt gcccgcgcca gcaaagctgc cggagataac
    66721 gcgatggcaa accagtacga aacactggca aatgcccgtc aaaaagggat cgaaaaatac
    66781 ctgtggaacg atcaacaagg ctggtatgcc gattacgacc tgaaaagtca taaagtgcgc
    66841 aatcagttaa ccgcggccgc cctgttccca ctgtacgtca atgcggcagc gaaagatcgc
    66901 gccaacaaaa tggcgacggc gacgaaaact catctgctgc aacccggcgg cctgaacacc
    66961 acgtcggtga aaagtgggca acaatgggat gcgccaaatg gctgggcacc gttacagtgg
    67021 gtcgcgacag aaggattaca aaactacggg caaaaagagg tggcgatgga cattagctgg
    67081 cacttcctga ccaatgttca gcacacctat gaccgggaga aaaagctggt ggaaaaatat
    67141 gatgtcagca ccaccggaac ggggggcggc ggtggcgaat atccattaca ggatggcttt
    67201 ggctggacca atggcgtgac gctgaaaatg ctggatttga tctgcccgaa agagcaaccg
    67261 tgtgacaatg ttccggcgac gcgtccactc agtgaatcaa caacacagcc ggtaaaacaa
    67321 aaagaggcgg aacctacgcc ttaactttgc tgtatatcga gcaatgccat tatgtgcata
    67381 aaattggcat tgctcttttt tcgccacgaa atgcccccca ttctacggtc tatgctttta
    67441 ttttcttata tcagagaaga ttttaatt
//
LOCUS       DAZKFZ010000004        66486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000004 DAZKFZ010000000
VERSION     DAZKFZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            19..603
                     /gene="yraH"
                     /locus_tag="ACHOQW_000218"
     CDS             19..603
                     /gene="yraH"
                     /locus_tag="ACHOQW_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9571365.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            683..1378
                     /gene="yraI"
                     /locus_tag="ACHOQW_000219"
     CDS             683..1378
                     /gene="yraI"
                     /locus_tag="ACHOQW_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9571366.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1407..3923
                     /gene="yraJ"
                     /locus_tag="ACHOQW_000220"
     CDS             1407..3923
                     /gene="yraJ"
                     /locus_tag="ACHOQW_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9571367.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3934..4667
                     /locus_tag="ACHOQW_000221"
                     /pseudo
     CDS             3934..4667
                     /locus_tag="ACHOQW_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(4710..5570)
                     /gene="rsmI"
                     /locus_tag="ACHOQW_000222"
     CDS             complement(4710..5570)
                     /gene="rsmI"
                     /locus_tag="ACHOQW_000222"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9571368.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5635..7671
                     /gene="lpoA"
                     /locus_tag="ACHOQW_000223"
     CDS             5635..7671
                     /gene="lpoA"
                     /locus_tag="ACHOQW_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP9571369.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7629..8024
                     /gene="yraN"
                     /locus_tag="ACHOQW_000224"
     CDS             7629..8024
                     /gene="yraN"
                     /locus_tag="ACHOQW_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP9571370.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            8044..8634
                     /gene="diaA"
                     /locus_tag="ACHOQW_000225"
     CDS             8044..8634
                     /gene="diaA"
                     /locus_tag="ACHOQW_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP9571371.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8644..9219
                     /gene="dolP"
                     /locus_tag="ACHOQW_000226"
     CDS             8644..9219
                     /gene="dolP"
                     /locus_tag="ACHOQW_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9571372.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9333..10373)
                     /gene="yraQ"
                     /locus_tag="ACHOQW_000227"
     CDS             complement(9333..10373)
                     /gene="yraQ"
                     /locus_tag="ACHOQW_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9571373.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10446..11081)
                     /gene="yraR"
                     /locus_tag="ACHOQW_000228"
     CDS             complement(10446..11081)
                     /gene="yraR"
                     /locus_tag="ACHOQW_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9571374.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11209..11727
                     /gene="yhbO"
                     /locus_tag="ACHOQW_000229"
     CDS             11209..11727
                     /gene="yhbO"
                     /locus_tag="ACHOQW_000229"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP9571375.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(11707..12150)
                     /gene="yhbP"
                     /locus_tag="ACHOQW_000230"
     CDS             complement(11707..12150)
                     /gene="yhbP"
                     /locus_tag="ACHOQW_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP9571376.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12201..12503
                     /gene="yhbQ"
                     /locus_tag="ACHOQW_000231"
     CDS             12201..12503
                     /gene="yhbQ"
                     /locus_tag="ACHOQW_000231"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP9571377.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(12490..12993)
                     /gene="yhbS"
                     /locus_tag="ACHOQW_000232"
     CDS             complement(12490..12993)
                     /gene="yhbS"
                     /locus_tag="ACHOQW_000232"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9571378.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(12987..13511)
                     /gene="ubiT"
                     /locus_tag="ACHOQW_000233"
     CDS             complement(12987..13511)
                     /gene="ubiT"
                     /locus_tag="ACHOQW_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP9571379.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            13720..14715
                     /gene="ubiU"
                     /locus_tag="ACHOQW_000234"
     CDS             13720..14715
                     /gene="ubiU"
                     /locus_tag="ACHOQW_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9571380.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            14724..15602
                     /gene="ubiV"
                     /locus_tag="ACHOQW_000235"
     CDS             14724..15602
                     /gene="ubiV"
                     /locus_tag="ACHOQW_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9571381.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            15683..16690
                     /gene="yhbW"
                     /locus_tag="ACHOQW_000236"
     CDS             15683..16690
                     /gene="yhbW"
                     /locus_tag="ACHOQW_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP9571382.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(16807..18051)
                     /gene="mtr"
                     /locus_tag="ACHOQW_000237"
     CDS             complement(16807..18051)
                     /gene="mtr"
                     /locus_tag="ACHOQW_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP9571383.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18205..20094)
                     /gene="deaD"
                     /locus_tag="ACHOQW_000238"
     CDS             complement(18205..20094)
                     /gene="deaD"
                     /locus_tag="ACHOQW_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP9571384.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20087..20167)
                     /gene="yrbN"
                     /locus_tag="ACHOQW_000239"
     CDS             complement(20087..20167)
                     /gene="yrbN"
                     /locus_tag="ACHOQW_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP9571385.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20274..21158)
                     /gene="nlpI"
                     /locus_tag="ACHOQW_000240"
     CDS             complement(20274..21158)
                     /gene="nlpI"
                     /locus_tag="ACHOQW_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP9571386.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21267..23402)
                     /gene="pnp"
                     /locus_tag="ACHOQW_000241"
     CDS             complement(21267..23402)
                     /gene="pnp"
                     /locus_tag="ACHOQW_000241"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9571387.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23649..23918)
                     /gene="rpsO"
                     /locus_tag="ACHOQW_000242"
     CDS             complement(23649..23918)
                     /gene="rpsO"
                     /locus_tag="ACHOQW_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9571388.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(24067..25011)
                     /gene="truB"
                     /locus_tag="ACHOQW_000243"
     CDS             complement(24067..25011)
                     /gene="truB"
                     /locus_tag="ACHOQW_000243"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9571389.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25011..25412)
                     /gene="rbfA"
                     /locus_tag="ACHOQW_000244"
     CDS             complement(25011..25412)
                     /gene="rbfA"
                     /locus_tag="ACHOQW_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9571390.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25577..28249)
                     /gene="infB"
                     /locus_tag="ACHOQW_000245"
     CDS             complement(25577..28249)
                     /gene="infB"
                     /locus_tag="ACHOQW_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9571391.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28274..29761)
                     /gene="nusA"
                     /locus_tag="ACHOQW_000246"
     CDS             complement(28274..29761)
                     /gene="nusA"
                     /locus_tag="ACHOQW_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9571392.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(29789..30241)
                     /gene="rimP"
                     /locus_tag="ACHOQW_000247"
     CDS             complement(29789..30241)
                     /gene="rimP"
                     /locus_tag="ACHOQW_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9571393.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30448..30524)
                     /locus_tag="ACHOQW_000248"
     tRNA            complement(30448..30524)
                     /locus_tag="ACHOQW_000248"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30488..30490),aa:Met,seq:cat)
     gene            30872..32215
                     /gene="argG"
                     /locus_tag="ACHOQW_000249"
     CDS             30872..32215
                     /gene="argG"
                     /locus_tag="ACHOQW_000249"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP9571394.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(32223..33848)
                     /gene="yhbX"
                     /locus_tag="ACHOQW_000250"
     CDS             complement(32223..33848)
                     /gene="yhbX"
                     /locus_tag="ACHOQW_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9571395.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34307..34393)
                     /locus_tag="ACHOQW_000251"
     tRNA            complement(34307..34393)
                     /locus_tag="ACHOQW_000251"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34357..34359),aa:Leu,seq:gag)
     gene            complement(34408..34740)
                     /gene="secG"
                     /locus_tag="ACHOQW_000252"
     CDS             complement(34408..34740)
                     /gene="secG"
                     /locus_tag="ACHOQW_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9571396.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(34968..36305)
                     /gene="glmM"
                     /locus_tag="ACHOQW_000253"
     CDS             complement(34968..36305)
                     /gene="glmM"
                     /locus_tag="ACHOQW_000253"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9571397.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36298..37146)
                     /gene="folP"
                     /locus_tag="ACHOQW_000254"
     CDS             complement(36298..37146)
                     /gene="folP"
                     /locus_tag="ACHOQW_000254"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP9571398.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37236..39170)
                     /gene="ftsH"
                     /locus_tag="ACHOQW_000255"
     CDS             complement(37236..39170)
                     /gene="ftsH"
                     /locus_tag="ACHOQW_000255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9571399.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39270..39899)
                     /gene="rlmE"
                     /locus_tag="ACHOQW_000256"
     CDS             complement(39270..39899)
                     /gene="rlmE"
                     /locus_tag="ACHOQW_000256"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP9571400.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40025..40318
                     /gene="yhbY"
                     /locus_tag="ACHOQW_000257"
     CDS             40025..40318
                     /gene="yhbY"
                     /locus_tag="ACHOQW_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9571401.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40474..40950)
                     /gene="greA"
                     /locus_tag="ACHOQW_000258"
     CDS             complement(40474..40950)
                     /gene="greA"
                     /locus_tag="ACHOQW_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9571402.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41198..42631
                     /gene="dacB"
                     /locus_tag="ACHOQW_000259"
     CDS             41198..42631
                     /gene="dacB"
                     /locus_tag="ACHOQW_000259"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9571403.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42671..43843)
                     /gene="cgtA"
                     /locus_tag="ACHOQW_000260"
     CDS             complement(42671..43843)
                     /gene="cgtA"
                     /locus_tag="ACHOQW_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP9571404.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43859..44824)
                     /gene="yhbE"
                     /locus_tag="ACHOQW_000261"
     CDS             complement(43859..44824)
                     /gene="yhbE"
                     /locus_tag="ACHOQW_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9571405.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(44951..45208)
                     /gene="rpmA"
                     /locus_tag="ACHOQW_000262"
     CDS             complement(44951..45208)
                     /gene="rpmA"
                     /locus_tag="ACHOQW_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9571406.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45229..45540)
                     /gene="rplU"
                     /locus_tag="ACHOQW_000263"
     CDS             complement(45229..45540)
                     /gene="rplU"
                     /locus_tag="ACHOQW_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9571407.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45799..46770
                     /gene="ispB"
                     /locus_tag="ACHOQW_000264"
     CDS             45799..46770
                     /gene="ispB"
                     /locus_tag="ACHOQW_000264"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP9571408.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            46999..47277
                     /gene="sfsB"
                     /locus_tag="ACHOQW_000265"
     CDS             46999..47277
                     /gene="sfsB"
                     /locus_tag="ACHOQW_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP9571409.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47325..48584)
                     /gene="murA"
                     /locus_tag="ACHOQW_000266"
     CDS             complement(47325..48584)
                     /gene="murA"
                     /locus_tag="ACHOQW_000266"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9571410.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48639..48893)
                     /gene="ibaG"
                     /locus_tag="ACHOQW_000267"
     CDS             complement(48639..48893)
                     /gene="ibaG"
                     /locus_tag="ACHOQW_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP9571411.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49053..49346)
                     /gene="mlaB"
                     /locus_tag="ACHOQW_000268"
     CDS             complement(49053..49346)
                     /gene="mlaB"
                     /locus_tag="ACHOQW_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP9571412.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49346..49981)
                     /gene="mlaC"
                     /locus_tag="ACHOQW_000269"
     CDS             complement(49346..49981)
                     /gene="mlaC"
                     /locus_tag="ACHOQW_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP9571413.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50000..50551)
                     /gene="mlaD"
                     /locus_tag="ACHOQW_000270"
     CDS             complement(50000..50551)
                     /gene="mlaD"
                     /locus_tag="ACHOQW_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP9571414.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50556..51338)
                     /gene="mlaE"
                     /locus_tag="ACHOQW_000271"
     CDS             complement(50556..51338)
                     /gene="mlaE"
                     /locus_tag="ACHOQW_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP9571415.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51346..52155)
                     /gene="mlaF"
                     /locus_tag="ACHOQW_000272"
     CDS             complement(51346..52155)
                     /gene="mlaF"
                     /locus_tag="ACHOQW_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP9571416.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52365..53342
                     /gene="yrbG"
                     /locus_tag="ACHOQW_000273"
     CDS             52365..53342
                     /gene="yrbG"
                     /locus_tag="ACHOQW_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP9571417.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53356..54342
                     /gene="kdsD"
                     /locus_tag="ACHOQW_000274"
     CDS             53356..54342
                     /gene="kdsD"
                     /locus_tag="ACHOQW_000274"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP9571418.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54363..54929
                     /gene="kdsC"
                     /locus_tag="ACHOQW_000275"
     CDS             54363..54929
                     /gene="kdsC"
                     /locus_tag="ACHOQW_000275"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP9571419.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            54926..55501
                     /gene="lptC"
                     /locus_tag="ACHOQW_000276"
     CDS             54926..55501
                     /gene="lptC"
                     /locus_tag="ACHOQW_000276"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP9571420.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55470..56027
                     /gene="lptA"
                     /locus_tag="ACHOQW_000277"
     CDS             55470..56027
                     /gene="lptA"
                     /locus_tag="ACHOQW_000277"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP9571421.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56034..56759
                     /gene="lptB"
                     /locus_tag="ACHOQW_000278"
     CDS             56034..56759
                     /gene="lptB"
                     /locus_tag="ACHOQW_000278"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP9571422.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56807..58240
                     /gene="rpoN"
                     /locus_tag="ACHOQW_000279"
     CDS             56807..58240
                     /gene="rpoN"
                     /locus_tag="ACHOQW_000279"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9571423.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58263..58550
                     /gene="hpf"
                     /locus_tag="ACHOQW_000280"
     CDS             58263..58550
                     /gene="hpf"
                     /locus_tag="ACHOQW_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP9571424.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58668..59159
                     /gene="ptsN"
                     /locus_tag="ACHOQW_000281"
     CDS             58668..59159
                     /gene="ptsN"
                     /locus_tag="ACHOQW_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP9571425.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            59205..60059
                     /gene="rapZ"
                     /locus_tag="ACHOQW_000282"
     CDS             59205..60059
                     /gene="rapZ"
                     /locus_tag="ACHOQW_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9571426.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60056..60328
                     /gene="npr"
                     /locus_tag="ACHOQW_000283"
     CDS             60056..60328
                     /gene="npr"
                     /locus_tag="ACHOQW_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP9571427.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60542..61174
                     /gene="yrbL"
                     /locus_tag="ACHOQW_000284"
     CDS             60542..61174
                     /gene="yrbL"
                     /locus_tag="ACHOQW_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP9571428.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(61171..61899)
                     /gene="mtgA"
                     /locus_tag="ACHOQW_000285"
     CDS             complement(61171..61899)
                     /gene="mtgA"
                     /locus_tag="ACHOQW_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP9571429.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(61896..62549)
                     /gene="elbB"
                     /locus_tag="ACHOQW_000286"
     CDS             complement(61896..62549)
                     /gene="elbB"
                     /locus_tag="ACHOQW_000286"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP9571430.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62779..65115)
                     /gene="arcB"
                     /locus_tag="ACHOQW_000287"
     CDS             complement(62779..65115)
                     /gene="arcB"
                     /locus_tag="ACHOQW_000287"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP9571431.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65211..66140)
                     /gene="yhcC"
                     /locus_tag="ACHOQW_000288"
     CDS             complement(65211..66140)
                     /gene="yhcC"
                     /locus_tag="ACHOQW_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9571432.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
BASE COUNT        16486 a        17924 c        16231 g        15845 t
ORIGIN      
        1 acaaggataa ggttataaat gaataaagtt acaaaaacag ctattgcggg cttacttgcg
       61 cttttcgcag gtaatgccgc tgcaaccgat ggtgaaatag tttttgatgg cgagatttta
      121 aaatccgctt gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat
      181 aatgccgaac agtttcgttc tgtaggcgat cgtagcccaa aaattccatt cactattcct
      241 ttagttaact gcccagtgac tgggtgggag cacgataacg gcaacgttga agcgtctttc
      301 cgtttatggc tggaaacgcg ggataatggt accgtcccta acttccctaa cttggcaaaa
      361 gtgggctctt ttgctggcac agcagcaaca ggtgtgggta tccgtattga cgatgcggaa
      421 agcggaaacc ttatgccact aaatgctatg ggcaatgata acacagttta tcagatccct
      481 gcggactccg ctggaattgt caatgttgac ctcatcgctt actacgtatc aaccgttgaa
      541 gcatccgaaa tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt
      601 taatacgtta cggcgttatc tgacctttca gataacgccc ttttccttcc tatttctcgt
      661 tgtatcaggt tgaaaaataa ctatgtcaaa acgaacattc gcgatgatat ttaccttgtt
      721 gtgtagcttc tgtatcggcc aggcgcttgc agggggtatc gttttacagc gaacgcgagt
      781 gatctatgat gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac
      841 accttattta ctgcaatcat gggtagataa tatagatggt acaagccgtg ccccatttat
      901 tataacccca ccgctaattc gtcttgaagc tggcgatgat tcatcactgc gaattattaa
      961 aacagctgat aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgtgccat
     1021 tccagcaaag aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac
     1081 acggatcaaa atgttttatc gccccgcaca cctgaaggga cgggtaagcg atgcgtggaa
     1141 atcactggaa tttaaacgta gtgatcattc actcaatata tataacccaa ctgaatatta
     1201 cgtcgtattt gccggactag cagtcgataa agccgatctc acaagcaaaa ttgaatatat
     1261 cgcgcccgga gaacataaac agttaccact tccagcatct ggcggaaaga acgtgaaatg
     1321 ggctgcgatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
     1381 aatataaaaa cacaggtcat cagggaatgc cacaacgaca ccaccaggga cataaacgca
     1441 caccgaaaca gttggcgctc attatcaaac gctgtttgcc gatggtgctc actggcagcg
     1501 gcatgctttg cactaccgct aacgccgaag agtattattt cgaccccatt atgctggaaa
     1561 ccacaaaaag tggtatgcaa acaaccgatc tgtcacgttt ttcaaaaaaa tacgcacaac
     1621 taccaggaac ttatcaggtt gatatctggc tgaataaaaa gaaggtttca cagaaaaaaa
     1681 ttacatttac cgccaatgca gagcaacttc tgcagccaca gtttacggta gaacaactac
     1741 gtgagctggg tattaaggtg gatgaaatcc cggcgctggc tgaaaaagat gacgatagcg
     1801 tgatcaactc gcttgaacaa atcattcccg gtacagctgc tgaatttgat ttcaatcatc
     1861 agcgacttaa tttgagcatt ccccaaattg cactgtaccg tgatgcaaga ggttacgtct
     1921 ccccttctcg ttgggacgat ggtataccaa cgctgtttac caactactcg tttacaggtt
     1981 ctgataaccg ttaccgccag ggcaatcgta gccaacgaca gtacctaaat atgcaaaatg
     2041 gtgccaattt tggcccctgg cgattacgta actattctac gtggacacgc aacgatcagg
     2101 cgtcaagctg gaacactatc agtagttatt tacaacgtga tatcaaggcg ttgaagtctc
     2161 agttgcttct gggagaaagc gccaccagcg gcagtatttt ttccagctac aactttactg
     2221 gcgtgcaact cgcttccgac gataatatgt tgccaaacag ccagcgcgga tttgccccaa
     2281 cggtacgcgg tatcgcaaac agtagtgcaa tcgtgactat caggcaaaat ggttatgtga
     2341 tctatcaaag caacgtgcca gcgggtgcct ttgaaattaa cgatctctac ccctcttcca
     2401 acagcggcga tttagaagtc acgattgaag aaagtgacgg tacgcaacgt cgctttatcc
     2461 agccttattc ttcattaccc atgatgcagc gacctgggca tctaaaatat agcgcgaccg
     2521 ctggacgcta tcgcgctgat gcaaacagtg atagcaagga acccgaattt gctgaagcca
     2581 cggcaatata tggtttgaat aatactttta cgctgtatgg cggcctgctc ggttctgaag
     2641 attattatgc gctggggatc ggtatcggcg gcacacttgg cgcactgggc gcgttgtcga
     2701 tggatatcaa cagagctgac acccaattcg ataaccagca ctcttttcat ggctatcaat
     2761 ggcgtacgca gtacatcaaa gatatcccgg aaaccaacac caatatcgct gtcagctact
     2821 atcgctatac caacgatggc tattttagtt ttgatgaagc caatacccgc aattgggact
     2881 ataacagtcg ccaaaaaagt gaaattcaat tcaacatcag ccagacaata tttgatgggg
     2941 taagtctgta tgcctccggt tcacagcaag actattgggg caataacgag aaaaacagga
     3001 atatctctgt tggggtttcc ggccagcaat ggggaattgg ttacagcctg aattatcaat
     3061 acagccgcta cactgatcaa aataatgacc gcgcactctc tttgaatctc agtattccgt
     3121 tagaacgctg gttaccgcgt agccgggttt cctatcagat gaccagccag aaagatcgcc
     3181 caacccaaca tgaaatgcgt cttgatggct cactgctgga tgatggtcgc ctgagctata
     3241 gtctggaaca aagtctggat gacgataaca accataacag tagcgtgaac gccagttacc
     3301 gttcacctta tggaaccttc agtgccggat acagttacgg taatgacagt agccaataca
     3361 attacggcgt taccggcggc gtggttatcc atcctcatgg tgtgacgctc tcgcaatatc
     3421 tgggcaacgc ttttgcgctt attgatgcta acggggcatc tggcgtgagg atacaaaact
     3481 atccggggat tgctactgat ccctttggct atgcagtggt tccttatctc acaacttatc
     3541 aggaaaaccg tctctcggta gatactacgc agctgcccga taacgtcgat cttgaacaaa
     3601 caacacagtt tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc aacgccaata
     3661 tcggttatcg cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg ccctttggcg
     3721 ctcttgccag caacgatgat acggggcaac aaagtatcgt cgatgagggc ggcatactat
     3781 atctctctgg gatatcgagt aaatcacaaa gctggactgt acgctgggga aatcaggcag
     3841 atcaacaatg tcagtttgct tttagtacac cggattcaga accaacaacc tctgtattac
     3901 aaggcacagc gcagtgccat taaggataaa aaaatgaaaa gagcgcctct tataacagga
     3961 cttttgttga tatccacatc ctgcgcttat gcctcctcag aagggtgtgg agctgacagc
     4021 actagcggtg cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa ccagacagat
     4081 aacgtgacag gacgggagtt tacctctgca acgctaagta gcactaactg gcaatacgcc
     4141 tgttcctgct ctgcgggtaa ggcagttaaa cttgtctata tggtcagccc cgtacttacc
     4201 accactggac atcagacagg atattacaaa ctcaatgaca gcctggatat taaaaccatg
     4261 ttattaaggt taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc
     4321 cagacggttt tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga
     4381 ttaaaaactc gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc
     4441 tggtcgccag acgaagaagt tcagacaatg cgcccgatgt cggtattcgt attgaaaatc
     4501 tcggcggcgg agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg
     4561 ggcatggcac cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa
     4621 caggaaaatt tcagggcaca gccaccatta ccgtcatcgt gcggtaaaag aagacgttgc
     4681 gttttgatta acgcaacgct tcggcactct tacccctgct gctccagcgc atacttatac
     4741 agcgcatttt tcttcacgcc gtgaatttct gcggccagcg ccgccgcttt tttcagcggc
     4801 agttctgcct gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt
     4861 gctttatgac cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt
     4921 acccacgcca gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc
     4981 tcacgcgcca gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta
     5041 tctaacagac ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct
     5101 tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg
     5161 tcagagggta aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc
     5221 accacacgaa tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt
     5281 agcggcgttc cggcatcgga aaccagcgca atgttttgcc cctcttgcag cttcgccagc
     5341 agcgtttcgg ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc
     5401 ccaaaatgtt gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg
     5461 gcctgtaata cctctaacgc acgctgggtg atatccgcca gattgccgat tggcgtcggt
     5521 acaatgtaaa gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc
     5581 gtattgccga tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta
     5641 ccctcaacat tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg
     5701 attttcgtcg gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg
     5761 gcgcaggctg attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg
     5821 atcaactggc aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg
     5881 gttgagttgt ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca
     5941 ctgctggcgg tagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg
     6001 gcgaaaatta cacccgccga tttagaacaa aaccagcaag cccgttactg gcaggcaaaa
     6061 atcgatgcca gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa
     6121 ccgctgcttg gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc
     6181 tccatgactc aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa
     6241 ggctggctgg atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa
     6301 gccgggatcg ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca
     6361 acgcagttgg ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg
     6421 ttgccactga atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcg
     6481 gcgaaaaata tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac
     6541 gtagcagaac aacctcagcc gcaaactgcg gatggcgttg ccagcccggc acaagcctcg
     6601 gttagcgatc tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg
     6661 acaagcaccg cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat
     6721 acctcatcac aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt
     6781 gtggttggtc cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa cactccgctg
     6841 aacgtactag cactgaacca gccggagaat atcgaaaatc gcgtcaatat ttgttacttc
     6901 gcgctttcac cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa
     6961 caagcgccgc tggtgctgat cccacgcagt tcattgggcg atcgcgtagc caatgcgttt
     7021 gcgcaagagt ggcagaaatt gggcggcggc accgttctgc aacaaaaatt tggttccacc
     7081 agcgaattac gcgcgggtgt taacggcggt tctggtattg ctttaacggg taccccgatt
     7141 actcccagag cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg
     7201 ccaaccgatg accagttcac caataatggc ggtcgtgtcg atgcggtgta cattgtggca
     7261 acgccgggtg aaatcgcttt tattaaaccg atgatcgcca tgcgtaacgg tagccagagc
     7321 ggtgcaacgc tgtacgccag ctcccgcagt gcgcaaggga ccgctggccc ggatttccgt
     7381 ctggagatgg aaggcttgca gtacagcgaa atcccgatgc tggcgggcgg taatctgccg
     7441 ttaatgcagc aggcactcag cgcggtgaat aacgattatt cactggctcg catgtatgcg
     7501 atgggcgtcg atgcctggtc gctggcaaat catttctcac aaatgcgcca ggttcagggt
     7561 tttgaaatca acggtaatac cggaagcctg acggctaacc cggattgcgt gattaacagg
     7621 aagttatcat ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac
     7681 agaccggcga tgcgtgggaa gtacaagcgc gtcgctggct ggaaggcaaa ggactgcgat
     7741 ttgtcgccgc taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc
     7801 ggaccaccgt ttttgtcgag gtgcgttacc gccgttctgc gctttatggc ggcgcggcag
     7861 ccagtgtgac ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc
     7921 gtcataatgg gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga
     7981 atgaggttga gtggattaag gataccttta atgaccactc ataattaagg tttaaggatt
     8041 agcgtgcaag aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg
     8101 gcagaggcgc ttccggatgc catctcccgt gcggccatga cgctggttca gtctctgctc
     8161 aatggcaaca aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt
     8221 gctgccagca tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta
     8281 aataccgata atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat
     8341 gcaaaacagg tgcgggcgct ggggcatgcg ggagatgtat tgttagccat ttccacccgt
     8401 ggcaacagcc gcgatatcgt aaaagcagta gaagctgccg ttacgcgtga tatgaccatt
     8461 gtggcattga ccggttatga cggcggtgaa cttgcaggtt tgttagggcc acaggatgtg
     8521 gagatccgca tcccttcgca tcgcagtgct cgcattcagg aaatgcatat gctgacggta
     8581 aattgcctgt gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa
     8641 tacatgaagg cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt
     8701 gttgccgctg ccgtagtggg tactgctgct gtgggtacca aagccgcaac tgacccacgc
     8761 agtgtcggca cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg
     8821 aaagacgaac agattaagaa agaagcgcgc attaatgtaa cggcctatca gggcaaagtg
     8881 ctgctggttg ggcagtcacc aaacgctgaa ctttcggctc gcgccaaaca gattgctatg
     8941 ggcgtagacg gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg
     9001 ggcgaagcgt ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc
     9061 gacctggtga aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg
     9121 gggctggtga ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc
     9181 gtgaagcggg taactacggc gtttacgttt attaaatagc agccccttgt aatgcctgat
     9241 gcgacgcttg ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg
     9301 tttacgccgc atccgacatg ttttccctca aatcagaata acagcgccag cccgccgaca
     9361 atcacgcctg acactgccac catcgccccc gtcagccata aggctttcgc cgggaacgct
     9421 ttgcgcagca taatcagtga cggcaaactc accgccggga gcgtcatcaa caatgccagc
     9481 gccggagcag ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc
     9541 gtgggaatga caaacaagca ccctgctacc gccatcgcca ccacccacat caggctgtta
     9601 tcgacagcac catcggcatg ggggaataac cagacgcgag cggcacccaa caccagtact
     9661 gcaaggatgt aaaccgggat cgtactccag aaaagcgtcc atagcgccct gccccagcga
     9721 ctaaaaaatc cgccctgtgc ttccggtatg tcaatttcga ccggtgcttg cgtttgcggt
     9781 gtttcacgca cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc
     9841 accagacgaa tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc
     9901 gggtttaaca ccggattgcc catccagaat gccagcgcac cgcccatcga cacctgttgg
     9961 cgacgcattc ccgccgcgac cggagccgca cagcaggtac acatcatgcc tggcagcgaa
    10021 aacagcgttc ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag
    10081 tcacgcggga tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc
    10141 cataccgcga ggaaatagat catcgcgtaa tccaacgccg cctgccatgg attagcatcc
    10201 gcctgcgcaa ggatagattt accgatactg tgggtttcgg cagcagtaaa cgctttgccg
    10261 tagtaaggtt gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg
    10321 ggtttccacc actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc
    10381 cccaggaata agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg
    10441 aataattatt cagctctttt gcgtaattct gaagagctta aaatcgtcac accttcatgc
    10501 tcgggccgca tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc
    10561 cagttaccgg gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg
    10621 ctacgatcgc ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggcg
    10681 attaatgctt cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg
    10741 gcacccatag cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg
    10801 gtatccacta ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc
    10861 cgcgtggtgc cgagacaaca aaacacaatg tcgatgggat cggtgacctg cgccagcgcg
    10921 tcagtcagtt gcggatcatg gggattaaat accccgggca tatcgcccaa cggacgtcgc
    10981 gtcggcgcgg ctatggcgtt aactttcggt tcgttaatca acatccgcag caggtgaccg
    11041 cccaccagcc ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat
    11101 tgtctgcctt gcgctccacg gctcatgcac caggcttaat agaccatgag gtaattatcc
    11161 ccgattgtgg gaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa
    11221 attgccgttt taatcactga tgaatttgag gattcagaat ttacttcacc cgcagacgag
    11281 ttccgtaaag ccggacacga agtgattacc attgaaaaac aagcgggtaa aacggtgaaa
    11341 ggcaaaaaag gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg
    11401 gagtttgatg ccctgttgct accgggcggt cattcaccgg attatctgcg tggcgacaac
    11461 cgttttgtca cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt
    11521 cacggtccgc agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcggtt
    11581 aaaccgatca ttattgatgt taaaaacgcg ggcgcggaat tttacgatca ggaagtcgtg
    11641 gtcgataaag atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc
    11701 gaggcgttac gcctgctcgg tgcctgagcc gcgtagccaa atcatttttt taccaaagcc
    11761 cagcgtgttg tcggtgaatt tgatttcatc gggccggatt tcccacaccg gggccgacag
    11821 cattctggca accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc
    11881 ttccagcctg cggatctcac ctttaaactg cacgccgcga attaacgcta ccgttttcgg
    11941 ctgaccgttt accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt
    12001 tttttcttcc gtcaaaatgt agaaggcgac tttctgcaca tcaaacagat aaaaggcatt
    12061 ggcgcaccag agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag
    12121 ccaacggctg atggcggtga gtgtttccat tgctgttctc ttttatactg tgggcctgac
    12181 cttaacatgc gacgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca
    12241 ataagcttta taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg
    12301 gcaaaggggc gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag
    12361 tgggcgatcg ttcgctggcg ttacgggcgg aatatcgcgt taagcaactg accaaacggc
    12421 aaaaagagcg tctggtagcg gaaggcgcag ggtttgcaaa gctgttaagc agtctgcaaa
    12481 ccccggagat taaaagcgat tgaaatgctc gtgatactca accagaccag ttacgccatt
    12541 caacgcgtca tccgctaaac gatgtacctg gaaggcgctt tcagtgcccg gccagcggca
    12601 acgcagatcg tgatgcgccg ccagttcaaa gccgaaacgg ctgtatagcg ccggatcgcc
    12661 cagcgtcacc actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag
    12721 ttggcgtgcc agcccctgtc cacggtattt ttcatctacc gccagcggtg ccatgccgac
    12781 ccattgcagg tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg
    12841 accttcgtca tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg
    12901 aaccagcttc gcttccgcat cactttcgaa tgaacgacgc agcagggcat caatacccgg
    12961 cgcatcaatg ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt
    13021 tcaggcgcgg ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt
    13081 aaggctttcg gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacagcccc
    13141 agctccgtat cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt
    13201 ttacgcgccg caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc
    13261 gcgttctggc tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata
    13321 tcacgcacat gaatacttaa ccagcggcct tcaagaaact ccagctcacc atcatccagc
    13381 gcctggcgga attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc
    13441 gtcagtttta ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt
    13501 ttatccaaca cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa
    13561 aataacatag cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac
    13621 tttaactgcc ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa
    13681 catttttgcg ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc
    13741 ggaaatctcc cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg
    13801 ctaaaagatg ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg
    13861 caggaagcgg tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact
    13921 tttgcgcatc cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg
    13981 ggtgccgacg cgctgatcct cgccgacctc gccatgctag agtacgccgc cgagcgttac
    14041 ccgcatattg agcgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac
    14101 ttttatcatc gccattttga cgttgctcgc gtggtgctgc cgcgcgtgtt gtcgattcat
    14161 caggtgaaac aactggcacg ggtcacacct gtcccgctgg aagtctttgc tttcggcagc
    14221 ctgtgcatta tgtcggaagg tcgttgctat ctgtcgtcgt atctgacggg tgagtcgccc
    14281 aacaccgtgg gcgcgtgttc tccggcccgt ttcgtgcgct ggcagcaaac gccgcagggg
    14341 ctggaatccc gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt
    14401 tatccgacgc tatgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa
    14461 gaaccaacca gtctcaatac cctggaactg ctgccggagt taatggcggc gaatattgct
    14521 tcggtgaaaa ttgaaggccg ccaacgtagc ccggcgtatg tcagccaggt ggcgaaagtc
    14581 tggcgtcagg ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg
    14641 tggatggaga cgctcgggtc gatgtccgaa ggcacgcaga ccacccttgg cgcgtatcac
    14701 cgtaaatggc agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc
    14761 caaaagagac gctggaagat ttttatcagc aggccgccac cagcagcgcc gacgtgattt
    14821 atcttggcga agcggtttgc agcaagcgcc gggcaaccaa agttggcgac tggctggaga
    14881 tggcaaaatc gctcgccgga agtggtaagc agattgtgct ctccacgctg gcgctggtgc
    14941 aggcatcatc tgaactgggc gaactgaaac gctatgttga gaacggtgag tttctgattg
    15001 aagccagcga tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg
    15061 gacacgcgct gaactgctat aacgcggtga cgctgaaaat attgctcaaa cagggcatga
    15121 tgcgctggtg tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt
    15181 gcgatgagct gggcattcgc aaccagtttg aagttgaagt cctgagctac ggtcatctac
    15241 cgctggccta ctccgctcgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt
    15301 gtgaaacctg ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac
    15361 aagtgtttgt actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg
    15421 agctggcatc catgcagggc ctggttgatg tggtccgcct gtcaccgcag ggtactgaca
    15481 ctttcgcgat gctcgacgcc ttccgcgcta atgaaaatgg cgctgcacca ctgccgttga
    15541 cggcgaacag cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct
    15601 aagtaaatag ctcactttgt taacaacttt aactactctt taatgcagta ttaaagatta
    15661 atcggtaaca aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct
    15721 ggcccccatt cccgaaggtt cttcagcgcg agatgcattc tcccactctc tcgatctcgc
    15781 ccgtctggct gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac
    15841 tggcattgcc agtgctgcca cgtcggtatt gattggctat ctggcggcga ataccaccac
    15901 gctgcatctg gggtctggcg gcgtgatgtt gcctaaccac tcaccgttgg tcattgcaga
    15961 acagttcggc acgctcaata cactctatcc ggggcgaatc gatttggggc tgggtcgtgc
    16021 tccgggtagt gaccagcgaa ccatgatggc gctacgtcgt catatgagcg gggatattga
    16081 taatttcccc cgcgatgtcg cggagctggt ggcctggttt gacgcccgcg atcccaatcc
    16141 gcatgtgcgc ccggtaccag gctacggcga gcaaatcccc gtgtggttgt taggctccag
    16201 cctttacagc gcgcaactgg cggcgcagct tggtctgccg tttgcgtttg cctcacactt
    16261 cgcgccggat atgttgttcc aggcgctgca tctttatcgc agcaacttca aaccgtcggc
    16321 acgactggaa aaaccatacg cgatggtgtg catcaatatt atcgctgccg acagcaaccg
    16381 cgacgctgaa tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga
    16441 aaccgggcaa ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca
    16501 gtatggcgtg caacaggcgc tgagtatgtc gctggtgggc gataaagcga aagtgcgtca
    16561 tggtttgcag tcgatcctgc gcgaaaccga cgccgatgag attatggtta acgggcagat
    16621 tttcgaccac caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt
    16681 gttgggatag tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcccacgt
    16741 aggcctgata agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggcc
    16801 cgctgattac tgatacaccg gcagcaaatt aaagctcgat aagatatgga ccagtgcgtt
    16861 gccaacgcca aataccagaa tcagcgtaat catcggcttg ccgccccaga cgcggaattt
    16921 cgggctgcca aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca
    16981 gatggtagct gctaaaccag cataaccaat ggcgtacagg aatccgttcg ggaacagcag
    17041 accacccaca acaggagggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga
    17101 gtcgtcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccgaggaa
    17161 cgaactcgct accgcaaagt ttgagaacac gaccagcagc agatccagac tacggctgtt
    17221 cagtacgccg cttaacgcct gtaccagcac atcaatatta ccgcccttct ctgcaatatc
    17281 gataaactcc ggacgcggga tgttacccat cgtcgccagc aaccagatgg tatacagcgc
    17341 cagcgccatc agcgtaccgt acaccagaca tttcacgatg gttttcggat ctttgccgta
    17401 atacttcatc aggcttggca cgttaccgtg ataaccaaac gatgccagac agaacggcaa
    17461 ggtcatcaac agatacggtg cataagacgc attgctttca gcgacgttga acaatgtcgc
    17521 aggctgcaca tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc
    17581 caatacaatc gctgtcatgc gactgacggc tttagtgctc aaccacacca caaacgctac
    17641 cagcaatgca aaaccaaaac ccgccgcccg tgccgggacg tttagtgaca tctctgcgaa
    17701 ggtgtgatgc agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac
    17761 aaaggcaatg gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat
    17821 ggtgtcaaaa ctcgaaccga ttctgtaatt caggttagct tccagaatca tcaagccgga
    17881 atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaaa accacgcccc
    17941 ggacatgacc actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac
    18001 cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc
    18061 tctccagtga aaaatagtgc gactgcgttg ttatgtattg cactgtacca gtacgcgagt
    18121 acaaaagaca gaaaaaaagc cccgatggtt caaatcgggg ctgtatatat tattttacag
    18181 actgtgttcg ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga
    18241 gtcatcacga cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc
    18301 acgaccacct tcacggcgtt caccgctgaa gttacgacca ccttcacgac gctcgccact
    18361 gaaaccacga ccaccgccac gacgctctcc gccagtatgc ggctgtgcat cgcccagtaa
    18421 ttgcatattc atcggcttgt tcagaatgcg agtacgtgta aagtgttgca gcacttcacc
    18481 cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttaa tgttaccgat
    18541 gtaacggctg ctgatgtcgc cttcgttagc aatcgcacca acgatatgac gaacttcaac
    18601 accatcatcg cggcccactt caatacggta caactgcatg tcaccaacat cacgacgttc
    18661 acgacgcgga cgatcttcac ggtcgccacg cggaccacgg tcgttacgat cgcgcggacc
    18721 acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat
    18781 cagagtacgt tcaccctgtg ccattttcag cagtgcggcg gccagagttt cgagatccag
    18841 ctcttcacct tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc
    18901 gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag
    18961 cagttctgcg ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt
    19021 gcgcagcaga cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc
    19081 acgaccggta cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt
    19141 aaccaccagg ctgatacgct caacgtccag gccacgggct gcaacgtcgg tcgcaatcag
    19201 gatgtccaga cgaccatctt tcaggcgttc cagtgtctgc tcacgcagcg cctggttcat
    19261 gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca agagcttcag ccacttccag
    19321 agtcgcattt ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa
    19381 acgtaccagt gcttcgtttt tacgcatacc ccagacagtc caatagctct ggctgatgtc
    19441 aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg
    19501 ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc
    19561 cgggatctgc gccataatag tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc
    19621 agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg
    19681 gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa
    19741 ttgcacgtca taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt
    19801 agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgctagcac
    19861 cagaatctgt ggtgctttca gctcaggatc aagattttgc agcagaggta aagagaatgc
    19921 tgcagttttt ccgctccccg tctgggccat acccagtacg tcgcggccat tcagcagatg
    19981 tggaatacac tccgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc
    20041 aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta
    20101 cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa
    20161 ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc
    20221 acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc
    20281 tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg
    20341 tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg
    20401 gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg
    20461 agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt
    20521 tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc
    20581 gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg
    20641 gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc
    20701 agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcgatccc gcgattcaag
    20761 tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca
    20821 tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc
    20881 ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta
    20941 tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga
    21001 ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat
    21061 ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt
    21121 gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg
    21181 ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc
    21241 gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt
    21301 gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg
    21361 cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact
    21421 ttctcaacgc gtttgtcagc gatttgagag atgtggacca gaccttcttt accgccgccg
    21481 atggcaacaa atgcgccgaa gtcaacgata cgggtcactt taccagtgta gacgcggccc
    21541 acttcaattt ctgcagtgat ctcttcgata cgacgaatag cgtgtttcgc tttctcgcca
    21601 tcggtcgctg cgatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct
    21661 tcggtcaggg cacggatcac agagccgcct ttaccgataa cgtctttgat cttgtccggg
    21721 ttgatcttga tggtatggat acgcggagcg aactcagaga tatcgccacg tggcgcgttg
    21781 atcgcctgtt ccattacacc caggatgtgc agacgcgcac ctttagcctg gttcagtgca
    21841 acctgcatga tctctttggt gataccttca attttgatat ccatctgcag tgcagagata
    21901 ccgtcgcggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa
    21961 atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca
    22021 acggcagctt tgattggcac acctgcgtcc atcagcgcca gagacgcacc gcacacggaa
    22081 gccatagaag aagaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg
    22141 aatttgtcca tatccggcat tactgccagc acgccgcgct tcgccagacg accgtgacca
    22201 atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag
    22261 ttgtagtgga acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg
    22321 tcacgagcag tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc
    22381 gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct
    22441 ttttcacgac catcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc
    22501 tcgatcgcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc
    22561 agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg
    22621 gtgatgcggt aagcatcgct cagacgagct tcagccagtg cagcaacgcg cgcgttcagc
    22681 gcttcgttta ccggctccgg ctgccagtcc caacgcggtt taccggcttc tttcaccagt
    22741 tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc
    22801 atctggtctt cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta
    22861 cccgcaacaa ccagatccag tttgctctct ttcagctcgt cctgagtcgg gttcagtacg
    22921 tactggtcat tgatgtaacc tacgcgggca gcaccaatcg ggccattgaa cggaatacca
    22981 gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacctgcggg
    23041 ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc
    23101 gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tggacgacct
    23161 tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta
    23221 acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca
    23281 aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg
    23341 ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc
    23401 aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat
    23461 cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc
    23521 tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc
    23581 cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact
    23641 cgcaagaatt agcgacgcag acccaggcgc tcgatgagcc gggtgtaacg tgctacgtct
    23701 ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga
    23761 cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca
    23821 gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca
    23881 gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag
    23941 aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa
    24001 caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg
    24061 ttaacattac gccgggtatt caaccaccag acgacgaggc gcaacgcggc cttcatcgtc
    24121 aatttcgccc ataccgataa acttgccgtt ctcaccttcc gtaacgcgaa ccagtccttc
    24181 cagcggcgcg ccagatgtac gaaccgggtt accatttttg aagtaaacag aagacgttaa
    24241 cggaagattc accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc
    24301 aagcaactcc gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcaggtg
    24361 ctccagggtc accatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat
    24421 cacatgcgcg ccacagccga gtttttcacc caggtcatca atgatggtgc ggatataagt
    24481 gccttttgag cagtgaattt ccagctccag ctcattgcct tcatggcgaa taaacagcaa
    24541 ttcataaacg gtaattggac gcgcttcacg cggaacttca atgccctgac gcgcatattc
    24601 gtacagtttt ttgccctggt atttcagtgc tgaatacatc gatgggatct gttcgatatc
    24661 gccacggaaa gtatccagtg ccgccgccag ttgttctgca ctaaaggtta ccggacgctc
    24721 ttcaacgatt tgtccgtcgg cgtcagaagt atcggtacgc tgtccaagac gcgcaatgac
    24781 ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca
    24841 aatcggcaac atgccggtcg ccagcgggtc cagcgcaccg gtatgcccgg cacgattggc
    24901 gttatatata cgtttcactt tttgcagcgc atcgttgctg gacataccct gcggtttatc
    24961 cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc
    25021 ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg
    25081 tttgacatac gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg
    25141 atacgcaggc gcatcgcttt acccagcagg ctgcggatga aaccagaagc ttcctgcaac
    25201 gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaaagt cacatacact
    25261 ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccaga
    25321 cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct
    25381 acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct
    25441 gttagcccct ttttaaaaat taatttcagg tggaagggct gttcacgttg gcctgataag
    25501 acgcgccagc gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca
    25561 tggaacccta aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga
    25621 tcacatcgcc agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt
    25681 tacggacttc gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcataga
    25741 taaccacgtt gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa
    25801 ccatacagcc tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca
    25861 gaccgataat ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt
    25921 cgtcgatcag gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca
    25981 ctttacgtgc agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg
    26041 cagccaaggt ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt
    26101 taacttcgtc agtagacagt ttcagcaagg agtcggagat cgcttcgaca gaaccctgta
    26161 cgtctgcctt caggacgata ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca
    26221 tgttctcgag tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac
    26281 gatagagtgc aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg
    26341 cagccggaac accggacaag ccgaggattt ccaccggaat ggacggaccc gcttccagca
    26401 cttcctgacc cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa
    26461 cgatatcgcc cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac
    26521 ctttatcgag gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca
    26581 gctccagaac ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccggtacccg
    26641 ctttcgcaga tacgtgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt
    26701 actgggagag ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca
    26761 ctgcaaccac caccggtacc tgcgccgctt tcgcgtgctg gattgcttcg atggtctgcg
    26821 gcattacgcc gtcgtcggca gcaacaacca ggactacgat gtcagttgcc tgcgcaccac
    26881 gtgcacgcat tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt
    26941 tttcagtttc aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg
    27001 ccactttcgt tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca
    27061 tgatggtcac aaccggagcg cgcggttcag ccgcagctcc cgtgtcacgg tcgctcatta
    27121 ccgcctcttc cagctcgttt tcacgacgca ggataacttt gtggcccatc tcttcagcaa
    27181 ccagctgtgc ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca
    27241 tcatcgcttt gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg
    27301 tgatagtttc gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt
    27361 gctgcagcga agaaccttta cgttttccgc ccttaccgcc acgtactgct gcgcgtgctt
    27421 cttcacgatc agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg
    27481 cgttacgacc acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc
    27541 gagcatgttg agaagtagtg acgtgataat cgctggaatc ctcagtcggt tccgcgttat
    27601 cagtccattt gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt
    27661 cttcgagttt acgacgcgct tcttcttcgg ctttacgctt gagctctgca gcttcctgct
    27721 cacggcgggc tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt
    27781 tgtctttttc cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg
    27841 cggcctcacg ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac
    27901 gggcttgctc ttccgcttca cgctgcgctt gctcttccgc tgcaaggcgt tcagcctctt
    27961 gcggatcgcg tttcacaaag gtgcgtttct tgcggacttc gatttgtacc gatttgcttt
    28021 ttccaccggt accaggaatg ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt
    28081 ccgggcctga atttttctga ttcaggtggt caatcaaagt ctgtttctct tgtgcagaca
    28141 cagagtcgtc agcagacttc cggatacctg catcagcaaa ttgctgtacc aggcgttcca
    28201 cggaggtctg tcgctctgcg gccagcgttt taatcgttac atctgtcatg ctgttccttc
    28261 ctgctacagt ttattacgct tcgtcaccga accagcaaat attacgggca gccataatca
    28321 gtgctccggc tttttcgtcg gtcaaccctt cgatatcagc cagatcatca atgccctgtt
    28381 cggcgagatc ttccagcgta caaacgccac gggcggccag tttgaatgcc aaatcacgat
    28441 ctaccccttc aaggttcagc agatcgtcag ccggtttgtt atcaccgagg ctttcttcct
    28501 gggcctgtgc aatggtggcc agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg
    28561 gctcatcaag gccttcgatt tccaacagct ctttcatcgg cacataggcc agttcttcca
    28621 gcgtcgagaa gccttcctct accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt
    28681 tggtgaaggt gtcgatcgct gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa
    28741 cggtcatcac gttgagttcc caaccgctca gctgcgaagc cagacgcacg ttctgaccgt
    28801 tacggccaat cgcctgcgcc aggttaccgg cttcaacggc gatatccatg gtgtgtttat
    28861 cttcatccac cacgatagaa gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg
    28921 ccgggttatc atcccacagg acgatatcga tacgctcgcc gcccagttca gtagaaaccg
    28981 cctgaacacg cgcgccacgc atacctacgc aagcacctac cggatcgata cgtttatcgt
    29041 tggttttcac cgcgattttc gcacgagaac ccgggtcgcg agccgctgct ttaatttcaa
    29101 tcacttcttc gccgatttct ggcacttcaa tacggaacag ttcgatcagc atttccggct
    29161 tggaacgagt gacgaacagt tgcgcgccac gcgcttccgg gcgaacggaa taaagcacgc
    29221 cacgaacgcg gtcgccaggg cggaagtttt cacgcggcag catatcttcg cgcaggatca
    29281 cggcttcagc gttgttgccc agatccagag agatgttgtc gcggtttact tttttcacca
    29341 cgccggtgat gatttcacct tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg
    29401 cttcacgcac tttctgcacg ataacctgtt ttgccgtctg ggtggtgata cggtcaaagg
    29461 taacagactc aatctgatct tcaacgtaat cgcccaggtt caggctttca tcttcataac
    29521 gtgcggcttc aagggtgatt tccttggtcg gctgggtgac ttcatcaaca actaaccagc
    29581 gacggaaggt gtcaaaatca ccgcttttgc gatcgatctg tacgcggacg tcgatctctt
    29641 gttcatattt tttctttgtt gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct
    29701 cgcgaggtag cgccttttca ttggatacgg cttcaactac agccaaaatt tctttgttca
    29761 tcgcgggctt ttcacctcat ccagactatt aaaagtgggg aaccaagttc gccttctgga
    29821 tattactcag cgcgaacact tcatctttac cttcgacggt aactgtgatc atttcaccgt
    29881 ctaccgcttt gataacgccc tgccatttac gacggttttg taccgccata cggagaacca
    29941 gagtcacctc ttctccgaca aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga
    30001 gacccggtga ggagacttcc aggttataag caacggtgat gggatcttca acatccagca
    30061 cagcacttac ctggtggctc acatcagcac aatcatcaac attgatgcca tcttcactat
    30121 caatatagat gcgcagtgtg gatgtgcgac cgcgaataaa ttcgatgcca accagttcaa
    30181 aacccagggc ctcaactggc gcagtaatca tctctgttaa tttttgctct aatgtggaca
    30241 agcccacccc caagacataa aaaaagggcc taaagcccag ttattctgta gtcagataac
    30301 aaaaaacccc gataaatcgg ggctttatat aactgaaccc tataaccgca actgcggtct
    30361 ggagcacttt ccagaaggat tttttcaaat cccactacga aggccgaagt cttcacagta
    30421 tatttgaaaa aggactctaa gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct
    30481 tcgggttatg agcccgacga gctaccaggc tgctccaccc cgcgcctgaa acgtggcaaa
    30541 ttctactcgt tttgggtaaa aaatgcaaat actgctggga tttggtgtac cgagacggga
    30601 cgtaaaatct gcaggcatta tagtgatcca cgccacattt tgtcaacgtt tattgctaat
    30661 cacgtgaatg aatatccagt tcactttcat ttgttgaata cttttgcctt ctcctgctct
    30721 cccttaagcg cattatttta caaaaaacac actaaactct tcctgtctcc gataaaagat
    30781 gattaaatga aaactcattt attttgcata aaaattcagt gagagcggaa atccaggctc
    30841 atcatcagtt aattaagcag ggtgttattt tatgacgacg attctcaagc atctcccggt
    30901 aggtcaacgt attggtatcg ctttttccgg cggtctggac accagtgccg cactgctgtg
    30961 gatgcgacaa aagggagcgg ttccttatgc atatactgca aacctgggcc agccagacga
    31021 agaggattat gatgcgatcc ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct
    31081 gatcgactgc cgcaaacaac tggtggccga aggtattgcc gctattcagt gtggcgcatt
    31141 tcataacacc accggcggcc tgacctattt caacacgacg ccgctgggcc gcgccgtgac
    31201 tggtaccatg ctggttgctg caatgaaaga agatggcgtg aatatctggg gtgacggtag
    31261 cacctataaa ggaaacgata tcgaacgttt ctatcgttat ggtctgctga ccaatgctga
    31321 actgcagatt tacaaaccgt ggcttgatac tgactttatt gatgaactgg gcggtcgtca
    31381 tgaaatgtct gaatttatga ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc
    31441 ttactccacg gactccaaca tgcttggtgc aacgcatgaa gcgaaggatc tggaatacct
    31501 caactccagc gtcaaaatcg tcaacccgat tatgggcgtg aaattctggg atgagagcgt
    31561 gaagatcccg gcagaagaag tcacagtacg ctttgagcaa ggtcatccgg tggcgttgaa
    31621 cggtaaaacc tttagcgacg acgtagaaat gatgctggaa gctaaccgca tcggcggtcg
    31681 tcacggcctg ggcatgagcg accagattga aaaccgtatc atcgaagcga aaagccgtgg
    31741 tatttacgaa gctccgggga tggcactgct gcacattgcg tatgaacgcc tgttgaccgg
    31801 tattcacaac gaagacacca ttgagcagta tcacgcgcat ggccgtcagt tgggccgtct
    31861 gctgtaccag gggcgttggt ttgattccca ggcgctgatg ctgcgtgact ctctgcaacg
    31921 ctgggttgcc agccagatca ctggtgaagt taccctggag ctgcgccgtg ggaacgatta
    31981 ttcaatcctg aataccgtct cagagaacct gacctacaag ccagagcgtc tgacgatgga
    32041 aaaaggcgac tcggtgttct cgccagatga tcgtatcggt caactgacca tgcgcaacct
    32101 ggatatcacc gatacccgcg agaaactttt cggttatgcc aaaactggcc tgctttcctc
    32161 ctctgccgct tcaggcgtgc cgcagatgga gaatctggaa aacaaaggcc agtaattcga
    32221 catcaaccct gcccttcagg ggtggggtta ccttgcttat cctctgtaaa ctcctttctg
    32281 agcatcacat aatcgaaaac atcatggttt gcatccacaa ccagtgagtc tcctttatag
    32341 tgtacaatca cttcctcaag cgtttgcggc tgttccggct ttgttacccc catccacgca
    32401 ttaataaggt aattattgta agctgtcgaa aagatgttgt tttccgttgt gcgatccaca
    32461 ccatccccaa gaacggggct ataccagata aacatcggaa catgatatgc ctgctggctg
    32521 gcctccctgc ctccatgaaa atagacgttc ttcttcgtag ggtcacgttc caggccgtgg
    32581 tcggcaaaat acatgaccga ggcgcggcga tcttttaata attcaaaaac ctgacctagc
    32641 aaactatctg tgtaatgaat ggagttgtca tagcaggcat cctgatcgtc ctgcggttga
    32701 aacacggcgc tggattgcgg ataggcgcta caagccggtt catggcttcc atttaaatga
    32761 agaacaatca gtttcttttg ctgcgtattt tgctgtaatg cttgcgataa atgcggcaac
    32821 aacaattcat caaatcctct gacataaact gtttccatgg cacgcatggc gatactggta
    32881 actgctgtac cattctgccg aaaagcggat tgcgagctta gccagaaagt ctgaaatcct
    32941 gcttgattag ccatattaat aatgttgtcg gggtaattat gaatgtcatg gctcaaaaca
    33001 gaatcagcag ttaaagaaag gggaaccgac agcgcagtgt aaggtgcacc gcttattgct
    33061 tgattaaaca gtttgatctg ttttctttgt gcttcgactt gcggtgtcgt agagcgtgta
    33121 tatccataca aagacatatt gtcgacacgt acagactccc ccacaatcaa cacgtaggta
    33181 tcaatacctg tatccctgac tgataattga aaatacggca ctgtgtttgc aattgagagt
    33241 aatctttgat gctctttcgc tgctaaagca aaataattga gattgaaaaa cggcgtatag
    33301 gtagcaaatc gcgacgctag tatatatgga ctgaacgcat ttttattttt ggcatcttta
    33361 taagcaaatt gacaagcgga aaataaactt cccgaaatga caatcagcaa taatattcct
    33421 gtcacttttt ttgtcggcaa ggaaacatca tattttatta ttacagccaa aaaaagaagg
    33481 gataaaaagg caaaagcaca tagataaggg ctatacattc ccagcatttt ggcaacttca
    33541 tccggatcac tctgcagcac actaatcgca aatccatcat tgaaagttgt accaaaggta
    33601 caccatgaat agaggctaat actcatgtct gccgcagtca gtaacacaaa tggtgcggca
    33661 acaagcaggc gaagcggaag ggatgatata cgtttaacaa ccaggatggt cagaacgaag
    33721 aacagcaatc gctgaaccat atgcgctccg gaagaggcga ctaagttcgt aataccaaaa
    33781 acaataacac taagatacaa caaccaatga gatttaaaac ttctagcaaa tttgttgaat
    33841 actgtcatcc agaaatccaa gttcacaacc aatataaaca attatcaaca atgtgcaatt
    33901 ttatatcaga catgtttttc aactctgatc catatcagaa cgaaacactg ttagtttttt
    33961 ctttttaaat gatgcggcca ttgcgttctc agtaattaaa taacattatc aatgcgtctc
    34021 taaacattac gcagaacgta gccatcagta gttatactga caggagcagg atggtttatc
    34081 gagaagtaaa catatccgta ttcgtaccgg aactggagtt gacacaataa agtgccaatt
    34141 atgtcagcag tagggaaaag tttataacga aggcgtaccg gcaccatcgt ttcaaggtac
    34201 cagctacgag taaagcaact ggacgagata cagatacctg acaaccattc ctcagaccag
    34261 gaccaaaacg aaaaaagacg cttttcagcg tctcttttct ggaatttggt accgaggacg
    34321 ggacttgaac ccgtaagccc tattgggcac taccacctca aggtagcgtg tctaccaatt
    34381 ccaccacctc ggcacggaaa ctacttttta gttcgggata tcgctggtcg gcttagccgg
    34441 agcagctggc tgagtttgtt cggttttcgc cggcgcactc aggttttccc attcgctacc
    34501 tttattggtc ttgttgctat tgatgttacc cagcaccaga ctgatgatga agaataacgt
    34561 tgccagcaac gccgtcatgc gggtcatgaa gttaccagaa ccacttgaac caaacagcgt
    34621 agcggaagcg cctgctccga aggaggctcc catatcagcg cctttacctt gctgtagcat
    34681 gatcagacca acaaggccaa ttgccacaat aaggaaaact accaaaagag cttcgtacat
    34741 aatcaacctg ttccttgcgg agttgccgcg taccaatgct tcaaccaata aagcgggagt
    34801 ttataatctt tcccactgaa gcgggtgtga atactaacca aaacgaatga ccttcgcaag
    34861 ggcaatttta tcgcattgta tcaactgcgg aaaaaaacag caaaaccgat gtgttcgccg
    34921 agaaaaaagg cgacaatcgc cgccttttta gccagttatc taacgcttta aacggctttt
    34981 actgcatcgg cgatgcggtg tgcaaattca gtcacctgcg cttcgtcttc gccttccacc
    35041 atcacgcgaa ttaacggttc ggtgccggat ttacgcaaca gcacacgtcc acggctaccc
    35101 agcgccgttt caacttctgc ggtcaccgct ttgactgatt catgctccag cggatcgccg
    35161 ctacctgcgg tgtaacgcac gtttaccagg atttgcggga acattttcat gccgctgcaa
    35221 aggtcgtgca ggctcatatg gttacgcgcc atcgccgcca gcacctgcaa accagcaacg
    35281 atgccgtcac cggtagtggt tttatccagc aggatcacat gaccggaatt ctctgcaccg
    35341 atacgccagc ctttctcctg cattttttcc agtacgtagc ggtcacccac tttcgcgcgc
    35401 gcaaatggaa ttcctaactg tttcagcgcc agttcaagcc ccatgttgct catcaatgta
    35461 cccacagcgc caccacgcag ctggccctga cgaagacctt cacgcgcgat gatatacatg
    35521 atctgatcgc catcgacttt attgccttca tggtcaacca taatcacgcg atcgccgtcg
    35581 ccgtcgaagg caataccgag atccgctttt tcagccagca cacgagcttg gagcgcgcga
    35641 acgtcggtag cccccacttc ggcattgatg tttacaccgt ttggctcaca accgatagcg
    35701 ataacattcg ctcccagttc gcgcagcacg ttcggcgcga tgtgataagt cgcaccgttt
    35761 gcgcaatcca ccacaatctt cagttcactg aggctaagtt cgttcgggaa cgtggctttg
    35821 caaaactcga tatagcgacc cgcagcatca acgatacggc tggctttacc cagttctgcc
    35881 gaatcaacac agctgatctc tttttccatt tccgcttcga tggcttcttc aactgcatcc
    35941 ggcagcttag taccgtcaat agaaaagaat ttaatgccat tatcgtagaa cgggttatgc
    36001 gatgcagata tcacaattcc ggcctctgcg cggaaggtac gcgtcagata agccacggcc
    36061 ggtgttggca tcgggccagt gaagagtgcg gaaaggcccg cagccgccag ccccgcttcc
    36121 agtgctgact ccagcatata gccagaaata cgcgtgtctt taccaataat aatcttacgg
    36181 gagccgtggc gcgccagcac tttacccgcg gcccaaccca gcttaagcac aaaatcaggt
    36241 gtgatcggcg catcccctac acgaccacga atcccatcgg taccgaaata tttacgatta
    36301 ctcatagcgt ttgttttcct ttgcagacag agtagcttcc accactcgca tcgcttctac
    36361 ggtttctttg acgtcatgaa cacgaataat gtgcgcgcct tgcattgcgg caatgaccgc
    36421 acaggccaga ctaccgctca ggcgctcgga cggccccacg ttcagcagct gcccaatcat
    36481 cgattttcgt gacataccca ccaacagcgg caggttgaaa tggtgaaatt cagccaggcg
    36541 cgccagtaat gaatagttat gggagagatt tttaccgaaa ccgaatccgg ggtcgagcaa
    36601 caatttctct tttgcgatac ccgcctgctc gcaacgtgct atttgctcaa taaagtagcg
    36661 attcacttct gcaaagacat cgtcatactt cggggcttcc tgcatggttt ttggatttcc
    36721 ctgcatatgc atcagacaaa ccggtaaacc ggtttctgca gccgcctcca gagcgccagg
    36781 ttcggaaagg gagcggatat cgttaataat gtgagcgcca actttcgctg actcacggat
    36841 gacttctggt ttggatgtat cgaccgagat ccagacttcg aagcgttgag caattgcctc
    36901 aaccacagga ataacacgtt gcaactcttc ttcaacgcta acttccgccg cccctggccg
    36961 cgtggactcg ccaccaacgt caatgatcgt cgcgccagcg ttgatcatca gattcgcatg
    37021 tttcaccgca tctatcagcg agttatgcgt gccaccatcc gaaaaggaat caggcgtgac
    37081 gttgaggatc cccattacgt gaggatggct aaggtccagt gaagtaccct gggcaaagag
    37141 tttcatgatg ttatccctgg tatgaattga taagaaaaaa ccccggagca cgccccgggg
    37201 ttttcggtac aaatacagtc atctgatgcg ggaacttact tgtcgcctaa ctgctctgac
    37261 atggtgttac ccgggttcgg cgtacgcggt tcatcaaccg gacgaggagc ctttggacta
    37321 ccattgtcgc cagcattgtt agaagcgcct ggttcttccc agcccgctgg cggacgtaca
    37381 tcgcgacgtg ccatcaggtc atcaatctgc ggtgcgtcga tagtctcata tttcatgaga
    37441 gcatctttca tcgcatgcag aatatccata ttgtcggtca gaagctgacg cgcacgatta
    37501 tagttacgct caatcagtgc tttcacttcc tggtcgatga tacgtgcagt ttcatcggac
    37561 atatgtttcg ctttcgctac gctacggccg aggaacactt caccttcttc ttccgcgtac
    37621 agcagtggac ccaatttctc agagaagccc cactgagtca ccatgttacg tgccaggttg
    37681 gtcgcaactt taatatcgtt ggacgcaccg gtagaaacat gttccggccc gtagatgatc
    37741 tcttctgcca gacgaccacc gtacagcgta gaaatctggc tttccagttt ctgacggctg
    37801 gcgctgattg cgtcgccctc aggcaagaag aaagtcacac ccagcgcacg accgcgtggg
    37861 ataatcgtca ctttgtgcac cggatcgtgc tccggcacca ggcgaccgat aatcgcatga
    37921 cccgcttcgt ggtaagccgt cgattctttc tgcgcttccg tcatcaccat ggagcgacgt
    37981 tccgcaccca tcatgatttt gtctttcgct ttctcgaact caaccatcga cacaacgcgt
    38041 ttgttgccac gagcagcgaa cagtgccgct tcgttcacca ggttcgccag gtcagcaccg
    38101 gagaaaccag gagtaccacg ggcaatgatt gccgcgtcga tatcgggtgc caatggtacg
    38161 cgacgcatgt gaaccttcag gatctgctca cgaccgcgaa catctggcaa gccaaccaca
    38221 acctgacggt cgaaacggcc aggacgcagc agcgctgggt cgagaacgtc cggacggtta
    38281 gtcgcggcga taacgatgat accttcgtta ccttcgaagc catccatctc aaccagcatc
    38341 tggttcagag tctgttcacg ttcatcgtga ccaccgccca gacctgcgcc acgctggcgg
    38401 cctacggcgt cgatttcatc gataaagatg atgcacggtg ccgctttctt cgcctgttcg
    38461 aacatgtcac gaacacggga tgcacccaca ccgacgaaca tttctacgaa gtcagaaccg
    38521 gagatagtaa agaacggaac tttcgcttcg cctgcaatcg ctttcgccag cagcgtttta
    38581 ccggtacccg gaggaccgac catcaggacg cctttcggga tcttaccgcc gagtttctgg
    38641 aagcggctcg gctcgcgcag atactcaacc agttcagcaa cttcttcttt tgcttcgtcg
    38701 cagcccgcaa cgtcagcaaa ggtcgttttg atctgatctt ccgtcagcat gcgcgcttta
    38761 ctcttaccaa acgacatggc acctttgcca ccgccgccct gcatttgacg catgaagaag
    38821 atccagacac caatcagcaa cagcatcggg aaccaggaga tgaagataga agccagcagg
    38881 ctgggttctt caggcggttc accgacaacc ttgacgttct tggtcaacag gttatccagt
    38941 aatttcggat cctgaaccgg aatgtaagtg gtataacggt tactatcttt cttggtaacg
    39001 ttgatttcac gtccgttgat acgcgcttca cgaacctggt cgttattgac ctcttgtagg
    39061 aaggtagagt aatccacctt acggccatta gactcgctgg gcccaaagct ctggaatact
    39121 gacatcagca caacggcaat gaccagccag agtattaggt ttttcgccat gtcactcaag
    39181 ggattaacct cttattacaa ctgtgttaaa aacagcgtca ggatactcta tatccagcat
    39241 ctttcaaact ttcgtctgaa atctcccggt tagggtttac gcccggtcgc tacaatatac
    39301 acttcccgcg aacgtgcacg agaagagtcc ggcttacgaa ctttgacctt cgtaaacagg
    39361 gagcgaattt cccttagata ctcatcgaaa ccttcgccct ggaacacctt cactacaaaa
    39421 ctgccacctg gcgctaatac atcacgacac atttctagcg ccagttccac cagatacatg
    39481 gcacggggga tatccaccgc cggtgttccg ctcatgtttg gtgccatatc ggacatgaca
    39541 acctggactt tgctgtcgcc aacgcgctcc agcagtgctt tcatcaccag ttcatcacga
    39601 aaatcgccct gaagaaagtc cacaccaacg ataggatcca taggtaaaag atcgcaagcg
    39661 atgatgcggc ctttgccgcc aatttgggtg accacatatt gtgaccaacc acccggagca
    39721 gcaccaaggt cgacaaccgt cattcccggt ttaaagagtt tgtcactttg ctgtatttca
    39781 tcaagtttaa accaggcacg ggaacgtaac ccctttttct gtgcctgttg aacatattta
    39841 tcgctaaagt gttcctgaag ccagcggctg gagctggcag aacgcttctt acctgtcatt
    39901 taactttccc atggggataa ctcatcgtaa ccaattgcgt aaatttttac gcgcctattt
    39961 ggtgatatat gggagatggc ggtagaatga cccgttttca atcccaacgt aagcaaaaaa
    40021 tacgatgaat ctgagtacta aacaaaaaca gcacctgaaa ggtctggcac atccgctcaa
    40081 gccagttgtt ctgcttggca gtaatggttt gaccgaaggg gtgctggccg agattgaaca
    40141 agcgttagag caccatgaac tcatcaaggt gaaaatcgcc accgaagatc gcgaaactaa
    40201 aaccttgatc gtggaagcta tcgtgcgcga aaccggcgcc tgtaatgtac aggtcatcgg
    40261 taaaacgctg gtgctttatc gcccaactaa agaacgtaaa atctcgctgc cacgctaaga
    40321 ttatcctaaa gttacacaca tttgctgtgt aaaacgaggg gttttccgca ggcaggagag
    40381 caaaatgcca cgctctgttc gttgataaaa ggccgctatg cggccttttt cctttcttta
    40441 caatacatca acatcttgag tattgggtaa ttcttacagg tattccacct taattacttc
    40501 aaattctact tcgccgcccg gcgttttgat gaccacaaca tcatcttctt ctttgccgat
    40561 caggccacga gcaataggtg agtttacaga aatcaggttt tgtttaaagt cagcttcgtc
    40621 atcgccaacg atgcgataag tctgttcttc gtcagaatcg agattcagca ccgttacggt
    40681 agcaccaaaa ataacgcgcc cattgttggg cattttggtg acatcaatca cctgcgcgtt
    40741 cgacagcttg gcttcgatgt ctttgatacg gccttcgcag aagccctgct gttcacgagc
    40801 tgcgtggtat tcggcgtttt ctttcaggtc gccatgctca cgcgcttccg cgatagcagc
    40861 aatgatttca ggacggcgca cagatttcag aaaatccagc tcttcgcgta atttttcagc
    40921 gccgcgtaag gtcatcggaa tagcttgcat ttgttatacc tcttgaatat tcctgatggg
    40981 gcaagtgttc acccaccccg gctgttcggc ctgcccggca taactcctca ccgggaccag
    41041 aagcaaaaaa ataccgaccc gggtacaagt cccaggtcag ctacaattca cattttgata
    41101 gtcattttac cctgaagttc ccgaagggtc atcgtttact ttatagggcg ttgcgccgta
    41161 gtatgacggc tcgattccag gttgttagcg cgagattatg cgattttcca gatttatcat
    41221 cggattgacc agctgtatag cgttcagtgt tcaggccgca aatgttgatg agtacattac
    41281 tcaactcccc gctggtgcca accttgccct gatggtgcaa aaagtcggcg cgtcggcccc
    41341 cgctattgat taccacagtc agcagatggc gctgcccgcc agtacccaga aagtgattac
    41401 tgcgctggcg gcgttgattc aactcggccc cgattttcgt ttcaccacga cgcttgaaac
    41461 caaaggcaat gtggaaaacg gcgtacttaa gggtgactta gtggcgcgat ttggtgccga
    41521 tccgacgtta aaacgtcaag atattcgcaa tatggtcgcg actttgaaaa aatctggcgt
    41581 caaccaaatc gatggcaatg tgttgataga tacctccatt ttcgccagcc acgataaagc
    41641 ccccggctgg ccatggaatg acatgacaca atgctttagc gctccgcctg ccgtcgccat
    41701 agttgaccgc aactgtttct ccgtctcgct ctacagtgcc ccaaaacctg gtgatatggc
    41761 ttttatacgc gtggcatctt attaccccgt tacgatgttc agccaggtac gcaccctccc
    41821 ccgtggttct gccgaagcgc aatactgcga actggatgtg gtgccaggcg acctgaaccg
    41881 ctttacgctg acgggatgcc tgccacaacg ttctgagccg ctcccgttgg cttttgccgt
    41941 gcaggatgga gccagctatg ccggtgcaat tctgaaagat gagttaaaac aggcgggtat
    42001 cacctggagc ggaacactgc tgcgccagac tcaggttaac gaacctggaa cggtagttgc
    42061 cagtaaacag tcggccccgc tgcacgatct gcttaagatt atgctgaaaa agtcggacaa
    42121 catgatcgcc gatacggttt tccgcatgat aggccatgcg cgcttcaatg tgcctggaac
    42181 atggcgggcc gggtcggacg ctgtgcgtca gatcctgcgc cagcaagccg gtgtcgatat
    42241 tggaaacacc attattgccg atggttcagg gctttcgcgg cataacctga ttgcccccgc
    42301 caccatgatg caggtgctgc aatacattgc ccaacacgac aatgaactta actttatctc
    42361 catgctgccg ctagcgggct atgacggctc tttgcagtac cgtgcaggtc tgcatcaggc
    42421 gggcgtggat ggaaaagtct cagcgaaaac cggttcgttg cagggggtat ataacctggc
    42481 gggattcatt accacagcga gcgggcaacg aatggcgttt gtacaatatc tttctggcta
    42541 tgcagtagaa ccggcggatc agcgtaatcg ccgtattccg ttagtgcgtt ttgaaagccg
    42601 tttgtataaa gatatttatc agaacaatta gtcaaaaaga aaccccggca catggccggg
    42661 gttttgctga ttaacgcttg taaatgaact caacgccttc ttcgtcgtct tcgtcccagt
    42721 cgtcatccca gtcctcatca tcctcttcag caatctcttc aagctgctgg cgatgataat
    42781 catcccacat gaattcgact ttctctggct gtttcgcttc ttcagcctgc acgaccgggt
    42841 tttcaatgat aaaggtcatc acatcccagc agagatcttt cacgcccagt ccactcgccg
    42901 cagagatcag ataatattta tcttcccagc ccagcgcctc tgcgatcgct ttcgctttct
    42961 cttcggcttc taccttatcc agcagatcga tcttgttgaa gactaaccaa cgcggtttcg
    43021 ccgccagatc ctggctgtat ttttccagct cgctgataat aatacgcgcg ttttcaaccg
    43081 gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa caggacgcgg caacgttcca
    43141 agtgcttcag gaagcgaatg cccagacctg cgccttccgc agcgccttca atcagtcctg
    43201 gaatatcggc aacaacgaag ctcttttcgt tgtccattcg taccacaccc agacttggca
    43261 ccagagtggt aaacggataa tccgccactt tcggtttagc cgccgatacc gcacgaataa
    43321 aggtcgattt acccgcgttt ggcatcccca acataccgac gtcagccagc agcatcagct
    43381 ccagcagcag ctcacgctta tcgcccggcg taccgttagt tttctgccgt ggtgtacggt
    43441 taactgacga tttgaaacgg gtattgccca gaccgtgcca gccgccctta gcaaccagca
    43501 gacgctgacc gtgtttggtc atatcgccca tggtttcacc agtaccctgg tcgattacac
    43561 gcgtacctac cggcacttta attgtgacgt ctttaccgcg cttaccggta cagtcgcggc
    43621 ttgcgccatt ctgaccgcgc tctgcacgga aagatttttc aaaacgataa tcgataagcg
    43681 tgttcaggtt ctcgtcggct tccatccata cgtcaccacc atcaccgccg tcgccgccat
    43741 ccgggccgcc tttcggaata tacttttcgc ggcggaagct cacgcaacca ttaccgccat
    43801 cacctgcaac gaccagaatc gatgcttcat caacaaactt cattttattc tccgtaaatc
    43861 attcgcctgc gcggggttgc gataccaccg ttgtatgctt acgtaatccg ccccaaatac
    43921 gatgaccaat ggcggaatac atcgcgcccg caaccacgac aaacgcaccg agataaccta
    43981 aaaggtttaa catcggtctg gcgaagaaat cgggccaggc cagtgataaa agatctgaaa
    44041 aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct cacctgcgct gcctgccagc
    44101 gagccatcgc ttccgccagg gcgccatatc ctaccaaggt attcagtccg caaaaaatta
    44161 aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc aggctttgcc agagggaaga
    44221 gcgcaattgt acataaagtg tacagtaaaa acaggatctg cggtgaggcc agccgacgca
    44281 ataaaacctt ttgcgccacg ccataactca cccaaaccgt cgcagcaccg accccaaaga
    44341 taactcccca ggtgtaatcg gttagctttg taaatatctc gaccagactg gtgttaaaaa
    44401 acatcaccag gccgctcagg agcatcaatg cccctacaac ctgagtgctg cgcattttct
    44461 ctttcaggat aaatacgctg gcaaccatca tgccaactgg cgagagttgc ccaatcacct
    44521 gcgaagcggt cggactcagg tattgcaagg atgagctgaa caggatgaag ttcccaaaca
    44581 gcccggcggt cgccactgcc aacaaaatca accagcgtgg cttacgaaac acgcgtaatg
    44641 gcggcaacct cttcttcacc gcaagaatgg cccccaggcc aatactcgcc atcaagaaac
    44701 ggtaaaacac gattgtcgga ggttccatca cctccagcac ctgcttcatt gcaattggca
    44761 acgcccccca gcaaattgct gtggtgagcg ccaaaagaat gccaatgcct gcctgctgct
    44821 tcataccgta ttccctgcaa agcgcatttg cggattaacg tcgcaccatg cgcattttta
    44881 ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg gctttcatcc gttaccggga
    44941 cgcgaaaaac ttattcagct tcgatgctga taaatttacg gtttttcggg cctttaactt
    45001 cgaatttcac tttaccgtct gctttagcaa acagagtgtg gtcacgaccg caacctacgt
    45061 tagcgccagc gtggaatttg gtaccacgtt gacgaacgat gatgctaccc gccagaacgg
    45121 attcgccacc gaaacgctta acgcccaggc gtttagcttc tgaatcgcga ccgttacgtg
    45181 tggagccgcc agccttttta tgtgccattt gaaatctctc ctcaggtctt aggcgctgat
    45241 gccagtaatt ttcacatcag tgaaccactg acgatggccc tgctgcttac gatagtgttt
    45301 acgacgacga aacttaacga ttttaacttt ctcgccacga ccgtgagcaa caacttcagc
    45361 tttgattacg ccgccatcaa cgaaaggaac gccgattttg acttcttcac cgtttgcgat
    45421 catcagcact tcagcgaact caacagtttc gccagttgcg atgtccagct tttccaggcg
    45481 aacggtctga ccttcgctta ctcggtgttg tttaccacca ctttggaaaa ccgcgtacat
    45541 ataaaactcc gcttccgcgc acaccttttc aatgattcag agtgcgctat aaatattcac
    45601 aatagggcgc gaatattacg caaaacgcac gcctttgaca agtgctacag tcaatacacg
    45661 aagaaaaaaa acacaacttg tacggtaacg tttatctgtg ccattttttc agtacaatca
    45721 ccctatattc ctaaccataa accctaagtt gcctttgttc acagtaaggt aatcggggcg
    45781 aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg agttaaccgc gcaagatatg
    45841 gcgggtgtta atgcggcaat ccttgagcag cttaattccg acgtccaact gatcaatcag
    45901 ttaggctatt acatcgtcag cggcggcggt aaacgtattc gtccgatgat tgctgtactg
    45961 gctgcacgag ctgttggcta tgagggaaat gcgcatgtca ccattgctgc cctgatcgag
    46021 tttatccaca cggcgactct gctgcacgac gacgttgtgg atgaatcaga tatgcgcagg
    46081 ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca gcgtgctggt aggcgatttt
    46141 atttataccc gcgcgttcca gatgatgacc agcctcggtt cactcaaagt gctggaagtc
    46201 atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc tgcaactgat gaatgttaac
    46261 gatccggaca tcactgaaga aaactacatg cgcgttatct atagcaaaac cgcgcgtctg
    46321 tttgaggctg ccgcgcagtg ttccggaatt ctggcgggct gtacgccgga ggaggagaaa
    46381 ggcctgcagg attatgggcg ctatctcggc actgctttcc agttgatcga cgatttactc
    46441 gattacaatg ccgatggcga acagttaggt aaaaacgtcg gcgacgatct gaacgaaggt
    46501 aaaccgacgc tgccgctgct gcatgcgatg catcatggca caccagaaca ggcacagatg
    46561 atccgtaccg ccatcgaaca gggtaacggt cgccatcttc tgaaaccggt tctggaagca
    46621 atgaacgctt gtggatctct tgaatggacg cgtcagcgcg ccgaggaaga agcagacaaa
    46681 gccatcgcag cgttacaggt gctcccggac accccttggc gagaagcact catcggcctc
    46741 gcgcacatcg ctgttcaacg cgatcgttaa tcccctcccc tcatcccgcg cagtgcgcgg
    46801 gatgattcca gtaaactcca taagaatacc tattcagcgt aataatgcac tgaaaaacca
    46861 gtgcatacat ctttatatat tctgaatatt cacactcttt ataggaactt tttagagcaa
    46921 tcggccatca ggagtatagt gatgctcgac agaagaagtg ttctgaatga aagcgactaa
    46981 cttaaggagt gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt
    47041 gcgggtttgc gcaagaaggg aacatcaatg gcggcggagt ctcgcagaaa tggtttgagt
    47101 tcctcaacgc tggcgaatgc attatcgcgc ccatggccga aaggagagat gattattgcg
    47161 aaagccctgg gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc
    47221 catgagttta tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg
    47281 gtctggcggt agccccgcga acggggctgc cagctctcag acgattattc gcctttcaca
    47341 cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta tccacgatcg
    47401 atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg
    47461 cttgctgatg cacgcagatc ggttgccata acctgtgcgc cagaaagttt ttcaacaccg
    47521 tgacaaataa cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc
    47581 acatgcataa agcggttttc aaagaccgtt tcggtgatga atccagtccc ttctgccacc
    47641 aggttcaaca gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta
    47701 cgtacgttaa cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg
    47761 acctcgatgt ccgctccagc gtcacgcagt ttcgccagca cggcgtcgag agtatctggc
    47821 tgcgtgttac ggcagataat tttgccgcga gaaatcgccg ccgccaccag gaaagtaccg
    47881 gtttcgatac gatccggtag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg
    47941 atgacgatac gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc
    48001 gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc
    48061 agggttgcag cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata
    48121 tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg
    48181 gtcgcgccta attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta
    48241 caaccgccag gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagtgcc
    48301 cagatagaag cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg
    48361 cgggcatcaa tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagc
    48421 ttcattgatg tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc
    48481 gccagtagag cggcaaaaag gataggcaga gcagcatttt tagcgccgga aattgtgact
    48541 tcgccctgga gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt
    48601 taacaattca tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga
    48661 tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc
    48721 gcaatatatt ccatcagcgg accatagacc gtctgctgtt ttttaacccg actcatgccg
    48781 tcaaacaact cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact
    48841 tcctggaggg agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct
    48901 tcaatcatca gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata
    48961 agcaaaaagc ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca
    49021 ggcggttaac gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa
    49081 ttatacaatt tcgccagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg
    49141 ccctgctttt tcgccagatc gataagatgg agcagcagtg ccagtccacc cgtatccacg
    49201 cgggacacac ggctaagatc gatgcaggta atccccttca ccgcttcctc acgcatttcc
    49261 caaagcggta gcagaacgtc ctgatccagc tctccggata acgccagtgt gtcacccgtc
    49321 tgcatccagc tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag
    49381 aaatcgattt cagttgcgca gtcaggccgt cgataccttt ggtacgtagc agcgttcccc
    49441 actcgttttg tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcctgcc
    49501 agttgcccgt ctgggagttt ttacgccact ggaagtccag acgcaccggc gggcggccat
    49561 tcgggtcaat aatggtaacg cgaataggca caatggtttt atcacccagc ggctgttctg
    49621 gcgcaatctg ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca
    49681 ggtactcacg gaaagcggca aagtaagcat cacgttgagc tggggtcgca ctcttgtaat
    49741 actggcccag caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat
    49801 caacaatggt acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc
    49861 gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata cggattggtc tggtctgccg
    49921 cggttgccgc actcagcggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca
    49981 tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac
    50041 ctggcgcagc agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa
    50101 ggaactgacc aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc
    50161 catccttcag gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat
    50221 attgttcccc cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatatggt
    50281 tataacgttg ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa
    50341 tatccgccac ccggcccaca acaacgccac caatactgac cggagagcgc gctttcaggc
    50401 cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg ttcagtacgt attgacgtca
    50461 cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac
    50521 ccacccaaat ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc
    50581 agcacaaaat ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc
    50641 cggctaatcc cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc
    50701 accgtgatgg cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg
    50761 acggcatttt gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg
    50821 accagcgatc cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa
    50881 ataaccccag cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc
    50941 tccatactgg agagttgctc tgtagcgcgc atcaggccga tttctgcggt tagcgccgaa
    51001 ccagcacgcc cggcaaacaa caacgcggca accaccggcc ccagttcacg cagtagcgat
    51061 aacgccacca gcatacccag actggtttcc gcactataag tggtcagaac cagataacct
    51121 tgcagcccca acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg
    51181 acgccgacat tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg
    51241 ccgaccagcg cattgaataa cattaacccg gcccgcccaa acgttctcag ggttttaatc
    51301 cctttatgtc cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa
    51361 aagatcagcg tgataatcgc cggcaggata gcggaacgga accggcccgt cagctatccc
    51421 gtccagaaac tgacgtacgc gcggatcagg attcgcctgc aacgcctggg cactgccatg
    51481 agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg
    51541 cacatcgtga gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag
    51601 cttcaccagt acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat
    51661 gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga
    51721 aagttcagaa ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat
    51781 caccgtacta tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac
    51841 gttgtcaaat acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg
    51901 tttgcgcact gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag
    51961 gatctcacca tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc
    52021 gatgcccgat ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt
    52081 atcgaagatg cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc
    52141 cacagactgc tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa
    52201 tttaaccttg aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc
    52261 atttgtttta ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa
    52321 aatcttcagc ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg
    52381 cactgttaat tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg
    52441 cgtctattct ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca
    52501 gtattggtac atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac
    52561 gcgatttagc cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg
    52621 gtctggctgc gctggttcgt ccttttaccg tccattccga tgttctacgc cgtgaattac
    52681 ccttaatgtt gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc
    52741 gcagcgatgg tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac
    52801 ttgcacgtca ggctgaacgt caggggactg acagcctgac cagagagcag cttgcagagc
    52861 tgccgcgtga cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca
    52921 tgccagtggc cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca
    52981 tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg aaccagcctg ccggaactgg
    53041 caaccgcaat agcgggcgtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg
    53101 gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acgccaggag
    53161 agattgatcc actggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt
    53221 ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa
    53281 ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat
    53341 aactggaaac gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca
    53401 ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat
    53461 cagaatttca cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg
    53521 gggatgggga aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt
    53581 acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg gtgatttagg catggtcacc
    53641 ccacaggatg tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cactgcctta
    53701 attccagtgc ttaagcgtct tcacataccg ttaatctgca tcaccggtcg cccggagagc
    53761 agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg
    53821 ttagggctgg caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct
    53881 gtcgcgctgt taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc
    53941 ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag
    54001 atcccgcatg ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa
    54061 aatcttggta tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac
    54121 ggtgatttac gccgtgtctt cgatatgggc gtggatgttc gtcagttaag tattgccgat
    54181 gtgatgacgc cggggggaat acgcgtgcgc cctggcattc tggccgttga ggcactgaac
    54241 ttaatgcagt cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt
    54301 gtgttacata tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa
    54361 caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta
    54421 tagcaaaagc agagaacatt cgtctgctaa tcctcgacgt tgatggcgta ctgtcagatg
    54481 gcctgattta tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt
    54541 atggcattcg ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg
    54601 ctaaactggt agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt
    54661 caaacaaact gatcgccttt agcgatctgc tggaaaaatt ggcgattgcc ccggaaaatg
    54721 tggcttatgt cggcgatgat ctcatcgatt ggccggtaat ggaaaaagtg ggtttaagcg
    54781 tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc cgattacgtg acgcgcattg
    54841 ctggcggtcg tggcgcagtg cgagaagttt gcgacttatt actcctggcg cagggcaaac
    54901 tggatgaagc caaagggcaa tcgatatgag taaagccaga cgttgggtta tcattgtgct
    54961 atcactggcg gttctggtga tgatcggtat taatatggcc gaaaaagacg ataccgccca
    55021 ggtggtcgtc aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta
    55081 taacccagaa ggggcgctaa gctatcgatt gattgctcaa cacgttgaat attattccga
    55141 tcaggccgtt tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc
    55201 gacatggtcc gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata
    55261 tggacacgtt gaagtcaacg cacttgtgcc ggactctcaa cttcgcagaa tcacgacgga
    55321 taacgcgcag atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata
    55381 cggaacaaca tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc
    55441 cgagctgatt gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta
    55501 atcttgtgct tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag
    55561 acactgatca gccgatacac attgaatcgg accagcaatc tcttgatatg caaggcaacg
    55621 tggttacctt taccggtaat gtcatcgtca cccagggcac cataaaaatt aatgccgaca
    55681 aagtggtcgt tacccgtccg ggtggcgaac aaggtaaaga agtgattgac ggctacggta
    55741 aaccggcaac gttctaccag atgcaggaca acggtaaacc cgttgaaggt cacgcttccc
    55801 agatgcacta cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc
    55861 aggtcgatag caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc
    55921 aggctttcag cgacaaaggc aagcgtgtaa ccaccgttct ggtgccgtcg cagctgcagg
    55981 acaaaaacaa caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa
    56041 cattaactgc aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca
    56101 gcctgaccgt caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga
    56161 ccaccacttt ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac atcattattg
    56221 atgatgacga tatcagtctg ctacctctgc atgcacgcgc gcgccgcggt atcggctatc
    56281 tgccacagga agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac
    56341 tgcaaattcg tgacgacttg tctgctgaac agcgtgaaga ccgcgcgaac gagctgatgg
    56401 aagagtttca cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac
    56461 gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg
    56521 aaccgtttgc cggggttgac ccgatctcag ttatcgacat taaacgcatc attgagcacc
    56581 tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa acactggcgg
    56641 tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat cgcccacggc acgcctacag
    56701 aaatcttaca agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat
    56761 agggtagaag tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt
    56821 gcaactcagg cttagccaac aactggcgat gacgccacag ctccaacagg caattcgtct
    56881 gttgcagttg tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc
    56941 gctgcttgag caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga
    57001 aacgctggac accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga
    57061 tgccagttgg gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat
    57121 tgacgacgag ctgccggtct atcagggcga aacgacgcag accttgcagg attacctgat
    57181 gtggcaggtc gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt
    57241 cgatgccgtt gatgacaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat
    57301 gggcgatgaa gagatcgaca tcgacgaagt tgaagccgtc cttaagcgga tccaacggtt
    57361 tgatccggtc ggtgtggcgg caaaagatct gcgtgactgc ctgctgatcc aactctccca
    57421 attcgataaa accacgccgt ggctggaaga ggccagactg atcattagcg atcatctcga
    57481 tctgttagcc aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt
    57541 gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg cgccccgggc aatcgatcca
    57601 gactggcgaa cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg
    57661 gacggtagaa ctcaacagtg acagcattcc tcgtctgcaa atcaaccagc actacgcctc
    57721 gatgtgcaat aacgcgcgca atgatggtga cagccagttt atccgcagca atctgcaaga
    57781 tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg
    57841 ctgtatcgtt gaacagcagc aagccttctt tgagcaaggc gaagaatata tgaaaccgat
    57901 ggtactggcc gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac
    57961 cacgcaaaaa tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag
    58021 tcacgtcaat accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cgctggtgaa
    58081 gaaattaatc gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt
    58141 gctgtcggaa caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt
    58201 atccattccg ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca
    58261 ctatgcagct caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg
    58321 ttacagccaa atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg
    58381 ttctgaaagt ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg
    58441 aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata
    58501 agctggcacg ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc
    58561 aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc
    58621 tccgagcctg ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg
    58681 atacaactct acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgtccact
    58741 gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc
    58801 taccgcctca ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta
    58861 tcggcaatgg tattgccatt ccgcatggca aactggaaga agatactctg cgtgccgttg
    58921 gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg
    58981 acttactttt tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt
    59041 cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga
    59101 gcgatgaaga gctgtatcaa atcattacgg ataccaaagg tactccggat gaagcgtagt
    59161 tattcggtaa tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg
    59221 tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt
    59281 tttactgcgt ggataacctt cccgtagtgt tgttacccga tctggctcga accctggccg
    59341 atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa
    59401 tattcgaaca ggcgatgagt aacctgcctg acgctttctc accgcaacta ctgttcctgg
    59461 atgccgaccg taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt
    59521 ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga aagcgatctg ctggagcctc
    59581 tgcgttcgcg agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggcag
    59641 aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga actgacaatg gtctttgagt
    59701 ctttcggctt caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct
    59761 tgccgaaccc gcactgggat ccgaaactgc gtccaatgac aggtcttgat aagcctgtcg
    59821 ccgcgttcct cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc
    59881 ttgagctatg gttacctatg ctggaaacca acaaccgtag ctacttgacg gtcgccattg
    59941 gttgtaccgg cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc
    60001 gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac
    60061 tgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa
    60121 gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac
    60181 cgaggctgaa gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca
    60241 gattgaagtt gaagcgaccg gtccacagga agaggaagca ctggccgccg ttatcgccct
    60301 ctttaattct ggttttgatg aagattaatc ttcatcactt tgacatacaa ctcccttcaa
    60361 actccccccg acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac gtcaattgta
    60421 catttcgtgc gctttttact ttcagaagaa ccttaagaaa accttaagaa gcattgttta
    60481 ggtttcgttt aagttaatcg accatactgg agatcgtcag aaaatatttc caggagatgg
    60541 catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga aatgttatgc
    60601 gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg atggtggcga
    60661 taaagagatc cgtcgtgagt taaagtacta cgcgcatctt ggtcgccggt taaaagactg
    60721 gagtggaata ccgcgctatc acggtaccgt cgagacggat tgcggaaccg gttatgttta
    60781 tgatgtgatt gctgattttg acggcaagcc ctccattacg ctgaccgaat ttgctgaaca
    60841 atgccgttac gaagaagacg ttgcacaact gcgccaatta ctgaaacagc tgaagcgtta
    60901 tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc tctgtcaccg
    60961 cattagtgag tcagaggtta tccccgtcgt ctgcgacaac attggtgaaa gcacattgat
    61021 tccactggcg acctggtcaa agtggtgttg tctgcgtaag caagagcggt tgtggaaacg
    61081 atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac ctcgtgagag
    61141 caaaacgctg gccctcgctt cgcgcgaagc ttaatccagc tggtgttgtt gcataaacgg
    61201 ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctgc gcacgtagcc
    61261 cgatggcgcg gagactttga aacgaagtgg attaggtaat acagctgcca gtaaggcagc
    61321 ttccgatcgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac
    61381 gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga tacgcttttt
    61441 gctccagaca gtttctatcc ccagcgttaa tccggcttcc agcccttttc gaacccagct
    61501 acgcccatcc cataaaaaga gatttttggc tgtctgttga gaaatcgttg aagcaccgcg
    61561 aatacggttt tcattgcgct cattgtgcgc cagggctttc tcaatggaag cgacatcaaa
    61621 gccccagtgc tcaggaaatg tctgatcttc tgcggcaatc accgccagtc ccatccacgg
    61681 cgagatttga tccatactga ccctgtcaga atgtgccacg taacgaaaat taccatgcag
    61741 ccaggcgctg acctgtcgct cgaccattac cgcagagaag ggaacaggtg caacgctaaa
    61801 caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca gaagaaaacg
    61861 gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac cagcacgcgg
    61921 gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag catatatgcc
    61981 ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg acacggcaca
    62041 tgctccgcgc ccatctcttc cagcacttct gcggtatcga tatcagtacc gatggtcaaa
    62101 cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat aaaaccaagc
    62161 ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc aacggtgcat
    62221 tcgcgaccaa gactggcaaa attgcttaaa ttctttgccg cgccaaaccc ccccggcaca
    62281 atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc accgcgcgtt
    62341 attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcttcgcc agttaaatga
    62401 ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt
    62461 gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata gacgccgcat
    62521 ccgctcagaa ttacgccaat tttcttcatt atgatcatcc tttcgcaatt gactgaaaca
    62581 cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca tgtaacaaat
    62641 catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgcagt gcccttgtaa
    62701 ctcatcataa taatttacgg cgcagccaag atttccctgg tgttggcgca gtattcgcgc
    62761 accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt tcagcacttc
    62821 tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt tatcttccca
    62881 ggcgggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc gcaaccccac
    62941 tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt tatcctgcgc
    63001 cgtcagattc gactccagca cgttgacata gcctggcatc atcttctcaa acaccgctaa
    63061 cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca tgggaatatc
    63121 gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct catcatcctg
    63181 ggtatcccag aatttcttga tcatcgcggt tagcgccgga acagaaagcg gcttactcag
    63241 cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa
    63301 ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc gtcagttcac gagagatatc
    63361 cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt cgcccggttt
    63421 aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac ctaatttttc
    63481 cagcacagaa cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca gcacattcag
    63541 cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg ctaccgacgg
    63601 tgcgtggatc gtcaacgtaa aggttgaacc tttaccctgt tcgctggtaa ccgtaatatc
    63661 gccgcccata tttttcgcca gacgacgaga aacggccaga ccaataccgg tgccggtggc
    63721 aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaagattt tatccagctc
    63781 atcctgcgga atgccgatac cagaatcttc cacttcaaaa tgcagcatat cgccttcatc
    63841 gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt tactgatgag
    63901 gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag
    63961 cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc
    64021 cagatcggca aggaagctgg tgaaatcaac cggttgatta tcaagctgga ccttgcgccg
    64081 ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga cggccgaaac
    64141 atggatggtc ttgagatatt tttcctgctc ggcggtgagt tcggtatcca gcagaatgcg
    64201 gctcaggccg acgataccgt tcagcggtgt acgcaattcg tggctgatgg tggaaataaa
    64261 cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga taccgcttac gctcggtaat
    64321 gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt acggcacttt
    64381 acgaatttca aagcaggctt tgcgtccgtc gggataatcc agccactgct cataggtcag
    64441 tgacacatta tgacggaaca ctttttcatc ggtttcaatg actttcgcag cggcttccgg
    64501 tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc cggtcagcag
    64561 ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa aaaccaggtc
    64621 gggtgaagca tcaaggaagg aacgtaaaaa tgaggattgc tgctcgagct gaatttgtgt
    64681 ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca gttctgcttc
    64741 cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt ttaactgaac
    64801 gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc gtgacagacg
    64861 ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg cccacggcgt
    64921 aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct gaccatgcag
    64981 caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca gcgccagcaa
    65041 cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact gcgccagcag
    65101 acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat acccaattct
    65161 gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac ctactccgtt
    65221 ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt cagatagcga
    65281 tccagctcga ccattcccgt ccatcgattt tcgcaccaca gaggagcaag cagcgtcggg
    65341 cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg gcgaatcatt
    65401 tctccggcag tgagcgtgta atcctccagt tcaataccgt tcaaacgccc cgcttcccag
    65461 gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat gccgtccacg
    65521 ccggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc gccaggcagg
    65581 ccgacaatta agtgagaaca caccttcagc ccgcgctgac gtgccagctg ggttgtacgc
    65641 tgataacagg caaaatcatg gccgcgatta atgcgatgca gggttttgtc gtgggcggtt
    65701 tgtagcccca gctccagcca cacttcgtag ccctggtcct tatattcgca aagcagatcc
    65761 agcaccgcat ccggcacgca gtccgggcga gtaccaacac acaaaccgac aatattggcc
    65821 tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa ggtgctggta
    65881 tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc
    65941 agttgctcgg caatggaacg atgttgctgc gcttcatcgg caaatgaggc aacattacag
    66001 aatgtgcagc cgccacgccc gatggtaccg tcacggttag ggcagctaaa accgccgtgc
    66061 agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc aaacatattg
    66121 actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc caaagcctgc
    66181 catttttagc tcaattcggc gatgacttgg atcaatcgtc ccggcctgct tttatcaact
    66241 gcataaccaa tcaaaattac cgaaatttca tgcataatca cataaatcac ttttgcttat
    66301 cttgtgtcag atttttttat ctccttatgg attttaggca aaacagtagc atgaaacgtc
    66361 attaccaatt aaggcagtat aaaatgctgg ttttctcgtc agttcaaggc aggataaggg
    66421 ttaacacgcc tttatgacag tcaggaattg gctgtttctc taacgacgcc ccttttagcc
    66481 ttaatt
//
LOCUS       DAZKFZ010000005        65946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000005 DAZKFZ010000000
VERSION     DAZKFZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            26..469
                     /gene="cmtB"
                     /locus_tag="ACHOQW_000289"
     CDS             26..469
                     /gene="cmtB"
                     /locus_tag="ACHOQW_000289"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP9571433.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            497..1885
                     /gene="cmtA"
                     /locus_tag="ACHOQW_000290"
     CDS             497..1885
                     /gene="cmtA"
                     /locus_tag="ACHOQW_000290"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP9571434.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1900..3177
                     /gene="yggP"
                     /locus_tag="ACHOQW_000291"
     CDS             1900..3177
                     /gene="yggP"
                     /locus_tag="ACHOQW_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9571435.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3174..4139
                     /gene="glpX"
                     /locus_tag="ACHOQW_000292"
     CDS             3174..4139
                     /gene="glpX"
                     /locus_tag="ACHOQW_000292"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9571436.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            4161..4670
                     /gene="fumE"
                     /locus_tag="ACHOQW_000293"
     CDS             4161..4670
                     /gene="fumE"
                     /locus_tag="ACHOQW_000293"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP9571437.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4667..5380
                     /locus_tag="ACHOQW_000294"
     CDS             4667..5380
                     /locus_tag="ACHOQW_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP9571438.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5352..6029)
                     /locus_tag="ACHOQW_000295"
     CDS             complement(5352..6029)
                     /locus_tag="ACHOQW_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9571439.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(6023..6700)
                     /locus_tag="ACHOQW_000296"
     CDS             complement(6023..6700)
                     /locus_tag="ACHOQW_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9571440.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6688..7395)
                     /locus_tag="ACHOQW_000297"
     CDS             complement(6688..7395)
                     /locus_tag="ACHOQW_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP9571441.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7396..7974)
                     /locus_tag="ACHOQW_000298"
     CDS             complement(7396..7974)
                     /locus_tag="ACHOQW_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9571442.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7997..8428)
                     /locus_tag="ACHOQW_000299"
     CDS             complement(7997..8428)
                     /locus_tag="ACHOQW_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP9571443.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8800..9819
                     /gene="epd"
                     /locus_tag="ACHOQW_000300"
     CDS             8800..9819
                     /gene="epd"
                     /locus_tag="ACHOQW_000300"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP9571444.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9869..11032
                     /gene="pgk"
                     /locus_tag="ACHOQW_000301"
     CDS             9869..11032
                     /gene="pgk"
                     /locus_tag="ACHOQW_000301"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9571445.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11247..12326
                     /gene="fbaA"
                     /locus_tag="ACHOQW_000302"
     CDS             11247..12326
                     /gene="fbaA"
                     /locus_tag="ACHOQW_000302"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9571446.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12390..12488)
                     /locus_tag="ACHOQW_000303"
                     /pseudo
     CDS             complement(12390..12488)
                     /locus_tag="ACHOQW_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12684..13544
                     /gene="mscS"
                     /locus_tag="ACHOQW_000304"
     CDS             12684..13544
                     /gene="mscS"
                     /locus_tag="ACHOQW_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9571447.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13683..14318
                     /gene="argO"
                     /locus_tag="ACHOQW_000305"
     CDS             13683..14318
                     /gene="argO"
                     /locus_tag="ACHOQW_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP9571448.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14411..15151
                     /gene="yggE"
                     /locus_tag="ACHOQW_000306"
     CDS             14411..15151
                     /gene="yggE"
                     /locus_tag="ACHOQW_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP9571449.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15318..16214
                     /gene="ygfI"
                     /locus_tag="ACHOQW_000307"
                     /pseudo
     CDS             15318..16214
                     /gene="ygfI"
                     /locus_tag="ACHOQW_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16211..17689)
                     /gene="scpC"
                     /locus_tag="ACHOQW_000308"
     CDS             complement(16211..17689)
                     /gene="scpC"
                     /locus_tag="ACHOQW_000308"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP9571450.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17713..18498)
                     /gene="scpB"
                     /locus_tag="ACHOQW_000309"
     CDS             complement(17713..18498)
                     /gene="scpB"
                     /locus_tag="ACHOQW_000309"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP9571451.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18509..19504)
                     /gene="meaB"
                     /locus_tag="ACHOQW_000310"
     CDS             complement(18509..19504)
                     /gene="meaB"
                     /locus_tag="ACHOQW_000310"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP9571452.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19497..21641)
                     /gene="scpA"
                     /locus_tag="ACHOQW_000311"
     CDS             complement(19497..21641)
                     /gene="scpA"
                     /locus_tag="ACHOQW_000311"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP9571453.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21845..22738)
                     /gene="argP"
                     /locus_tag="ACHOQW_000312"
     CDS             complement(21845..22738)
                     /gene="argP"
                     /locus_tag="ACHOQW_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP9571454.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23121..23780
                     /gene="rpiA"
                     /locus_tag="ACHOQW_000313"
     CDS             23121..23780
                     /gene="rpiA"
                     /locus_tag="ACHOQW_000313"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9571455.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24035..25267
                     /gene="serA"
                     /locus_tag="ACHOQW_000314"
     CDS             24035..25267
                     /gene="serA"
                     /locus_tag="ACHOQW_000314"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9571456.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25496..25555
                     /gene="ibsC"
                     /locus_tag="ACHOQW_000315"
     CDS             25496..25555
                     /gene="ibsC"
                     /locus_tag="ACHOQW_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP9571457.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25889..25948
                     /locus_tag="ACHOQW_000316"
     CDS             25889..25948
                     /locus_tag="ACHOQW_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9571458.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(26049..26651)
                     /gene="fau"
                     /locus_tag="ACHOQW_000317"
     CDS             complement(26049..26651)
                     /gene="fau"
                     /locus_tag="ACHOQW_000317"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9571459.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26672..26855)
                     /gene="ssrS"
                     /locus_tag="ACHOQW_000318"
     ncRNA           complement(26672..26855)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOQW_000318"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26897..27226)
                     /gene="zapA"
                     /locus_tag="ACHOQW_000319"
     CDS             complement(26897..27226)
                     /gene="zapA"
                     /locus_tag="ACHOQW_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9571460.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27394..27972
                     /gene="ygfB"
                     /locus_tag="ACHOQW_000320"
     CDS             27394..27972
                     /gene="ygfB"
                     /locus_tag="ACHOQW_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP9571461.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            27998..29323
                     /gene="pepP"
                     /locus_tag="ACHOQW_000321"
     CDS             27998..29323
                     /gene="pepP"
                     /locus_tag="ACHOQW_000321"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP9571462.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29320..30498
                     /gene="ubiH"
                     /locus_tag="ACHOQW_000322"
                     /gene_synonym="visB"
     CDS             29320..30498
                     /gene="ubiH"
                     /locus_tag="ACHOQW_000322"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP9571463.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30522..31724
                     /gene="ubiI"
                     /locus_tag="ACHOQW_000323"
                     /gene_synonym="visC"
     CDS             30522..31724
                     /gene="ubiI"
                     /locus_tag="ACHOQW_000323"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP9571464.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31798..31881)
                     /locus_tag="ACHOQW_000324"
     CDS             complement(31798..31881)
                     /locus_tag="ACHOQW_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9571465.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32172..33266
                     /gene="gcvT"
                     /locus_tag="ACHOQW_000325"
     CDS             32172..33266
                     /gene="gcvT"
                     /locus_tag="ACHOQW_000325"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP9571466.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33290..33679
                     /gene="gcvH"
                     /locus_tag="ACHOQW_000326"
     CDS             33290..33679
                     /gene="gcvH"
                     /locus_tag="ACHOQW_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP9571467.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33797..36670
                     /gene="gcvP"
                     /locus_tag="ACHOQW_000327"
     CDS             33797..36670
                     /gene="gcvP"
                     /locus_tag="ACHOQW_000327"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP9571468.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(36836..38269)
                     /gene="bglA"
                     /locus_tag="ACHOQW_000328"
     CDS             complement(36836..38269)
                     /gene="bglA"
                     /locus_tag="ACHOQW_000328"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP9571469.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            38314..38625
                     /gene="yqfB"
                     /locus_tag="ACHOQW_000329"
     CDS             38314..38625
                     /gene="yqfB"
                     /locus_tag="ACHOQW_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP9571470.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            38789..39448
                     /gene="yqfA"
                     /locus_tag="ACHOQW_000330"
     CDS             38789..39448
                     /gene="yqfA"
                     /locus_tag="ACHOQW_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9571471.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(39525..40505)
                     /gene="ygfZ"
                     /locus_tag="ACHOQW_000331"
     CDS             complement(39525..40505)
                     /gene="ygfZ"
                     /locus_tag="ACHOQW_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP9571472.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            40748..41014
                     /gene="sdhE"
                     /locus_tag="ACHOQW_000332"
     CDS             40748..41014
                     /gene="sdhE"
                     /locus_tag="ACHOQW_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP9571473.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            40995..41402
                     /gene="ygfX"
                     /locus_tag="ACHOQW_000333"
     CDS             40995..41402
                     /gene="ygfX"
                     /locus_tag="ACHOQW_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP9571474.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(41442..41963)
                     /gene="fldB"
                     /locus_tag="ACHOQW_000334"
     CDS             complement(41442..41963)
                     /gene="fldB"
                     /locus_tag="ACHOQW_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP9571475.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            42075..42971
                     /gene="xerD"
                     /locus_tag="ACHOQW_000335"
     CDS             42075..42971
                     /gene="xerD"
                     /locus_tag="ACHOQW_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9571476.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            42996..43706
                     /gene="dsbC"
                     /locus_tag="ACHOQW_000336"
     CDS             42996..43706
                     /gene="dsbC"
                     /locus_tag="ACHOQW_000336"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP9571477.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            43712..45445
                     /gene="recJ"
                     /locus_tag="ACHOQW_000337"
     CDS             43712..45445
                     /gene="recJ"
                     /locus_tag="ACHOQW_000337"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9571478.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            45536..46634
                     /gene="prfB"
                     /locus_tag="ACHOQW_000338"
     CDS             join(45536..45610,45612..46634)
                     /gene="prfB"
                     /locus_tag="ACHOQW_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9571479.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            46644..48161
                     /gene="lysS"
                     /locus_tag="ACHOQW_000339"
     CDS             46644..48161
                     /gene="lysS"
                     /locus_tag="ACHOQW_000339"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9571480.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48204..48752)
                     /gene="idi"
                     /locus_tag="ACHOQW_000340"
     CDS             complement(48204..48752)
                     /gene="idi"
                     /locus_tag="ACHOQW_000340"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9571481.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            48807..48878
                     /locus_tag="ACHOQW_000341"
     CDS             48807..48878
                     /locus_tag="ACHOQW_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9571482.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(48875..49000)
                     /gene="yqfG"
                     /locus_tag="ACHOQW_000342"
     CDS             complement(48875..49000)
                     /gene="yqfG"
                     /locus_tag="ACHOQW_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP9571483.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49002..50450)
                     /gene="uacT"
                     /locus_tag="ACHOQW_000343"
     CDS             complement(49002..50450)
                     /gene="uacT"
                     /locus_tag="ACHOQW_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP9571484.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            50886..52805
                     /gene="ygfT"
                     /locus_tag="ACHOQW_000344"
     CDS             50886..52805
                     /gene="ygfT"
                     /locus_tag="ACHOQW_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP9571485.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            52805..53293
                     /gene="ygfS"
                     /locus_tag="ACHOQW_000345"
                     /pseudo
     CDS             52805..53293
                     /gene="ygfS"
                     /locus_tag="ACHOQW_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(53329..54696)
                     /gene="ghxQ"
                     /locus_tag="ACHOQW_000346"
                     /gene_synonym="ygfQ"
     CDS             complement(53329..54696)
                     /gene="ghxQ"
                     /locus_tag="ACHOQW_000346"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP9571486.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(54732..56048)
                     /gene="guaD"
                     /locus_tag="ACHOQW_000347"
     CDS             complement(54732..56048)
                     /gene="guaD"
                     /locus_tag="ACHOQW_000347"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP9571487.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(56066..57466)
                     /gene="xanQ"
                     /locus_tag="ACHOQW_000348"
     CDS             complement(56066..57466)
                     /gene="xanQ"
                     /locus_tag="ACHOQW_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP9571488.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGALLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(57631..60501)
                     /gene="xdhD"
                     /locus_tag="ACHOQW_000349"
     CDS             complement(57631..60501)
                     /gene="xdhD"
                     /locus_tag="ACHOQW_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP9571489.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(60498..61277)
                     /gene="ygfM"
                     /locus_tag="ACHOQW_000350"
     CDS             complement(60498..61277)
                     /gene="ygfM"
                     /locus_tag="ACHOQW_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP9571490.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(61327..62655)
                     /gene="ssnA"
                     /locus_tag="ACHOQW_000351"
     CDS             complement(61327..62655)
                     /gene="ssnA"
                     /locus_tag="ACHOQW_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP9571491.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(62658..65756)
                     /gene="ygfK"
                     /locus_tag="ACHOQW_000352"
     CDS             complement(62658..65756)
                     /gene="ygfK"
                     /locus_tag="ACHOQW_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP9571492.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT        15975 a        16536 c        17552 g        15883 t
ORIGIN      
        1 cctaaaaata aaataaggat aatttatgcg gcttagtgac tattttccag aatcatcaat
       61 ctcagttata cattcagcaa aagattggca ggaagctatc gatttctcga tggtatcatt
      121 gctggataaa aactatatca gcgagaatta cattcaggca attaaagatt ccaccattaa
      181 caatggccct tattatattc tcgcaccagg cgtggcaatg cctcatgcgc gaccggaatg
      241 tggggcgctt aaaaccggga tgtctttgac attacttgaa caaggtgttt attttccggg
      301 gaatgacgaa ccaattaaat tactcatcgg actctctgct gccgatgccg attcgcacat
      361 tggcgctatt caggcgttaa gtgaattact gtgcgaagaa gaaatactcg aacaactctt
      421 aacagcatca tcagaaaaac aattagcgga cattatcagc cgcggataat tactctctcc
      481 tctacaggat aatattatgg aaaacaagtc tgctcgtgca aaggtccagg cttttggggg
      541 ctttttgact gcaatggtca tccccaatat tggtgctttt attgcctggg gttttattac
      601 tgcgttattt attcccaccg gttggctgcc taacgaacat ttcgccaaaa ttgtcggccc
      661 gatgattacc tatttattgc ccgtgatgat tggttctacc ggtggtcatc tggtcggcgg
      721 taaacgcggc gcggtcatgg gcggaatagg tactattggt gtgatcgtcg gcgcagagat
      781 cccgatgttc cttggctcaa tgattatggg gccgctcggt ggtctggtca taaaatatgt
      841 tgataaggca ctggaaaaac gcatacctgc cggttttgag atggttatca ataacttctc
      901 attaggtatc gcggggatgc tcctttgcct gctgggtttt gaagtgatcg gcccggcggt
      961 gttaattgcc aatactttcg tcaaagagtg tattgaggcg ctggtacatg cgggttatct
     1021 gcctctgctg tcagtcatca atgaaccggc gaaagtgctt ttccttaata atgcgatcga
     1081 tcagggcgtc tattatccgc tgggaatgca acaggcttcg gttaacggta aatccatctt
     1141 ctttatggtg gcctctaacc caggtccggg cctggggctg ctgctggcgt ttaccttgtt
     1201 tggtaaaggg atgagtaaac gttctgcgcc cggggcgatg attattcact tcctcggtgg
     1261 gatccacgaa ctgtatttcc catatgtgct gatgaagccg ctgactatta ttgccatgat
     1321 tgcgggcggt atgtctggca cctggatgtt taacttactg gacggtggtc tggtggctgg
     1381 cccaagtccg gggtctatct ttgcttacct ggcgctgacg ccgaaaggtt cgttcctggc
     1441 gacaattgcc ggtgttacgg taggtaccct ggtgtccttt gctatcactt cgctgatact
     1501 gaagatggaa aaaacggtgg aaacggagag cgaagatgag tttgctcagt cagccaatgc
     1561 ggttaaggcg atgaaacaag agggtgcatt ctcgttaagc agggttaagc gtatcgcctt
     1621 tgtttgcgat gcggggatgg gctccagtgc gatgggcgcg accaccttcc gtaaacgcct
     1681 ggaaaaagcg gggctggcaa ttgaagtaaa acattacgcc atagaaaacg tgcctgcgga
     1741 tgcggatatc gtcgttactc atgccagtct ggaagggcgc gtgaaacgtg tgacggataa
     1801 accactgata ttgattaata actatattgg cgatccaaaa ctcgacactt tatttaatca
     1861 attaaccgcc gaacataaac actgattaca gaggtaaaaa tgaaaaccaa agttgctgct
     1921 atttatggca agcgggatgt ccgtctgcgc gaatttgaac tgccagaaat taccgataat
     1981 gaattactgg tgagtgtgat ttctgacagc gtctgtttat ccacctggaa agcggcgtta
     2041 ctcggtagtg aacataaacg cgtacccgac gatttagaaa atcatccggt cattaccggg
     2101 catgaatgtg ccggggttat tgtcgaagtg ggtaaaaatc tcactggcaa atataaaaaa
     2161 ggccagcgtt ttgtattgca accggcgatg gggttaccaa gcggatattc agcgggctac
     2221 agctacgaat attttggcgg caatgccact tatatgatta ttcccgaaat cgccattaat
     2281 ttgggctgcg tattaccgta tcacggctct tattttgctg cggcgtcgct ggcagagcct
     2341 atgtgctgca ttattggcgc ttatcatgcc aattatcaca ccacgcaata tgtttatgag
     2401 catcgcatgg gcgtcaaacc tggcggcaat attgcactgc tggcgtgtgc aggtccgatg
     2461 ggcattggcg ctatcgatta cgccattaac ggcggcatac aaccgtcgcg ggtggtggtg
     2521 gtcgatatcg acgacaaacg tctggcgcag gtacagaagc tgctgccggt ggaactggcg
     2581 gccagtaaag gcattgagct ggtgtatgtg aataccaaag ggatgagcga tcctgtccag
     2641 atgctgcggg cgctgacagg agatgccggg ttcgatgaca tttttgttta tgcggcggtg
     2701 tctgctgtcg ttgagatggc tgatgaatta ctggcggaag atggctgtct gaacttcttt
     2761 gccgggccga cggataaaaa cttcaaagtg ccgtttaatt tctacaacgt tcattacaac
     2821 agcacgcacg tcgtcggtac atccggcggt tcaacggacg acatgaaaga ggcgattgcc
     2881 cttagcgcca cagggcagtt acagccctcg tttatggtga cccatatcgg tggcctggat
     2941 gcggtgccag aaaccgtgct caatctgccg gatatccctg gcggtaaaaa actcatttat
     3001 aacggcgtga ccatgccgct cactgccatt gccgattttg ccgaaaaagg caaaaccgat
     3061 ccgctgttta aagagttggc gcggctggtt gaggaaacgc acggcatctg gaatgaacag
     3121 gccgagaaat atctgctggc acaatttggc gttgatatcg gggaggccgc gcaatgatgt
     3181 ccctggcgtg gccattattt cgcgttacgg aacaggcagc gttggctgcc tggccgcaaa
     3241 ccggatgtgg cgacaaaaat aaaattgatg gcctggcggt caccgcgatg cgccaggcat
     3301 taaacgacgt cgcttttcgt ggacgagtgg ttatcggcga aggagaaatt gaccatgcgc
     3361 cgatgctgtg gattggcgaa gaggtgggca aaggcgatgg gccagaagtc gatatcgcgg
     3421 ttgatcccat tgaaggcacg cggatggtgg cgatggggca gagcaatgcg ctggcggtca
     3481 tggctttcgc ccctcgagat agcctgttac atgcgcccga tatgtatatg aaaaagctgg
     3541 ttgttaatcg actggctgcc ggggcgattg atctgtcttt gcctctggcg gacaacttgc
     3601 gtgacgtggc gcgggcgtta ggtaaaccgc tggacaagct gcgcatggtt acgctggata
     3661 aaccgcgtct gagtgccgcg attgaagaag caacacagct gggcgtgaag gtttttgccc
     3721 tgccggatgg cgatgtcgcc gccagcgtgc tgacctgctg gcaggataat ccttacgatg
     3781 tgatgtacac catcggcggt gcgccggaag gcgtgatttc tgcctgtgcc gttaaagcat
     3841 taggcgggaa tatgcaggcc gagctgattg acttttgtca ggccaaaggg gattacacgg
     3901 aaaatcggca gattgcagag caggagcgca agcgttgcaa ggcaatgggt gttgacgtta
     3961 accgtgtgta ctcgctcgat gtactggtaa gggggaacga tatcctcttt agcgctacgg
     4021 gcgtcacggg cggcgagttg gtgaacggta tccaacagac ggcgaatgga gtgcggacgc
     4081 agacattact gatcggcggc gcggaccaaa cgtgtaatat aatagactcc ctgtattgat
     4141 ggtgatctat cggaagacca atggcgacgc tgtcagaaga tgatgtgctt gagcaactgg
     4201 atgcacagga caatttattt tcatttatga aaactgcgca ttctattttg ctccagggga
     4261 tacgccagtt tctgccgtcg ctgtttgtcg ataacgatga agagagcgtc gaatatgcag
     4321 tgaagccgtt actcgcccaa agcggcccgc ttgacgatat tgatgttgca ctgcgtttga
     4381 tttatgcgct ggggaaaatg gataaatggc tgtacgccga tatcacgcat ttttcccagt
     4441 actggcatta cctgaaagaa caggatgaaa cgctcggatt tgccgatgac atgacttggg
     4501 attttatcag caacgtcaat agcataacct gcaatgcgac gctctatgac gcgttgaaag
     4561 cgatgaagtt cgccgatttc gccgtctggt cagaggcgcg ttttagcgga atggtcaaaa
     4621 cggcgctgac gctggcagta acgaccacct taaaggaatt aacgccgtga aaattgaatt
     4681 aacggtgaat gggctgaacg tgcaagcgca gtaccatgat gaagagatag aacgcgtgca
     4741 taaaccgttg ctgcgtatgc tggcggcctt acagacggta aatccgcagc gccgaaccgt
     4801 ggtttttctt tgcgctccgc cggggacggg taaatctacc ctaaccacct tctgggcata
     4861 tctcgcgcaa caagacccgg agctgcccgc tattcagacg ctcccgatgg atggttttca
     4921 ccattacaat agctggctgg atgcgcatca attgcgcccc ttcaaaggcg caccagagac
     4981 atttgacgtt gcgaaactgg cggaaaatct gcgccaggtc gtggaagggg attgtacgtg
     5041 gccgcagtac gatcgacaaa agcatgatcc tgttgaagat gcgttgcacg ttaccgcacc
     5101 aataatcatc gtcgaaggaa actggctatt gctggatgac gataactggc gccagctagc
     5161 gcaattttgt gatttctcga tctttatcaa tgcgccagct actgcgttgc gggaacgact
     5221 ggtggggcgc aagctggcgg gagggttatc gctggcagac gcagaagctt tttatgaccg
     5281 taccgacggg ccgaatgttc gccgggtgct ggaagagagt ctgcctgcaa atttgacctt
     5341 aatgatgacc gctacgggtg aatatcgtct ggtggattaa tgttcctgat tagaccatct
     5401 tccagtcgca ccacacgaga gaaatgacgc cgggtaaatg cggcgtcatg actgattgct
     5461 acgacgctcg tgcctcgctg gcggcacttc tccagccagc tttcaaacac gctcaaccaa
     5521 ttttcgtcaa aatcccgact cggctcgtcc agcagcaata gtggcggaga gagtgcttcc
     5581 aggcaggcga cggcgaccat ccgtcgctgc gcactatgca gatccaacgg gtgcgcgctg
     5641 gcagtgtctg ctaactgaca acattgcagg gcagcatggg tacgttgagt gatttcatcc
     5701 gccgggcatt tttgcagttt caaaccaaat gcgatctcgt cagcgacggt gctgtgaaaa
     5761 agttggtttt ccgcttcctg aaacagtacg ccaatctttg ccgcgcgctg gcggtttttc
     5821 aggtttttca tcgcctgttg ttgcagcata accgtaccgg ctgtgggcgt aagaagacct
     5881 gccattactc gtaacagcgt cgatttccct gcgccattgt cgccggtcaa cgccaaccat
     5941 tcaccttgtt tgagttgcag agaaatgtcg caaagacaat ccgttgccgc gccaggccag
     6001 cgataagata tctgatttaa cgttaacata gtggctggag cgctccgttt tgcaattgcc
     6061 acgcttgctg gcagtagttc aggaaaggtg tgcgatgacg ttcaaaaaga ataatcagcg
     6121 aaccacgttc aaacgcccag tgttgtaagc gctgcaacag catttcgctg gcctgcggcg
     6181 tcaggcggct aaaggcttca tcaagaatta atagctttgg ctgcatcgcg atggcgcagg
     6241 cgatgaccac gcgctgtgtc tctccaccgg agagtgttgc cgggtgacga tggcgcaacg
     6301 gctggcactc ggtcagagtc agggcggcat caatccgcgc cataatctct gcttcagcaa
     6361 gacacagatt ttctggccca aaggcaactt cttcttccac gctaaaggta cagccggaga
     6421 gctgcaaata gggcgattgc tggacgagct ggatggtggc ggattgttca ttaagcggga
     6481 gatgcccaat gggcgtaccg aggagcgtcc ccgtgcctgt tatctctcct ggcagaaagt
     6541 ccggatacca gcccgccatc agctgtgcca gcgtactttt gccactgccg ttgtcaccaa
     6601 agatggcgac catccccggt ttgacatagt gaaagtcata acagaacgga ggatgcgttg
     6661 agtgtgtcgg gcaataacgg aactgttcta acgtaaccat atccacgctc cgaattctgt
     6721 cagcatcagc aagatcatgg tgtaacgcgc tacgcgttgt agggtgctat cggctggtgc
     6781 ccacaacgtt gttcgtcggt tgtggatccg aaatgcccgc atatccagcg ccgcaccacg
     6841 gacagcaaga tcgtttagcg cattgtgtgt tagcggaata gttaacgcgg gcatcgcacg
     6901 taaacgctga taccagccct catccagcgg cacgccgcgg gcacgctgcg cttcatgaat
     6961 aatcgctaat tgccgtttta actgctcaac gaccaataac ggcccggcaa acagataagc
     7021 gacgcccggc ggcaggcgag aagcaaacag agcgcggata aaacattgca cggggacgta
     7081 ctgcatccac aactgtgagg ttgaaacaat cgccagaatc cgcaaccaga gcgtaatagc
     7141 atgcgtccaa cgctccggcg aacgcggcgt gccgcttagc cattctgtta gccagccacc
     7201 gtgtaccagc cacagcccgg ctcccagcga gaacatcagc cagacgacat attttgcccg
     7261 ccgccgcgtg gcctttagcg caataaggca aaaaaaggtc gccgcgctat agatcggcaa
     7321 aatggtctgc gcgggcagga taagcgtggt gcaggccgcc aatgcccata gtgttaatga
     7381 ggtaaatgga tgcatttagc gcacggcagc cattgccgga aaatggcgcg tggtgcgaat
     7441 cggcagctgg cgcagtaaaa gccagacaat caccgctgta agagttttat cgaccaggtt
     7501 cgcgcctatc accgtaatcg ccaccgactc caccaggttt tgccccatcg agtgcatcca
     7561 ggcaacgaac aaatcggctc cgctaccggt gacaccgccg aacagcgcag tacgcagcgg
     7621 tacagcgacg accgttaccg caagcgtaat aatcacgccg ctgacaacga cttttggcag
     7681 ggtgcgaaac caacccgctc gcgccagcca accggcgacc aggccgatga ccatcgcgac
     7741 gggggcaaaa gcggcagcga tcgggtcggt cagtaatccc cagagtaaat tggtcagtaa
     7801 gccggtcagc atcccgatca ccggaccaag cagtaccgcg ctaataagtg tgccgatgga
     7861 gtcgagaaat ataggtaatt tgaccatgct ggccagttgc ccgccgatca tattgatggc
     7921 gatagatata acgatcaaca ccagtgcctg actggaaaag tggctacgcg ccataagaaa
     7981 acctctctga aagtagttat ttaacggggg aaatatgcaa ctcgtcgtaa ccagaaagcg
     8041 cgcaaggttg gcggctaaac gccagttcct caaggctacc gatcaccagt tcaagcgtgg
     8101 tccgcacggt acagccgggc atcatatgac cgccaagcat cgtgccgtgt ggatcggaaa
     8161 cgcagagatg aagatgctca ccactctgtt ccaacgtgcc gtttagtgag atcacttcga
     8221 atgtaccgtt caggagcgtg gtgccttctt gcccggcata gcgcaaggcg atgtcagtca
     8281 ggctaccggt acagcctgca atccaggcgg catgaagttg ctgctgctgt gcaaaagcgt
     8341 gtagctggga aaaaacttcc tgcccgggga ggaggcgcag tgcgtaaaag cgtgcggagg
     8401 aagaatatgg gtagggcaat gtcatcattg gagtactccg aaactcggac gtattacgga
     8461 taatatcgac gctgcatcag tcatattctg atataagtca gggtttgtga cggcacaacg
     8521 aaaagatttt gctacgctta ttcgtgattc tgcaaagtgg aataaattcc acaggggcgg
     8581 ggctgaaaac gtgacgtaag tcagataatt gttttctttg actggacaat cattcctttt
     8641 attccacgtt tcgcttatcc tagctgaagc gtttcagtcg attaaatgtt cgacaattaa
     8701 ccaatcagtc gcagtttgcg acaggtaagg tttccccgga cgatttgctg gattactctg
     8761 tctggcaaat ttgctcaggg aaaaccttgc aggagatcta tgaccgtacg cgtagcgata
     8821 aatggcttcg gtcgcatcgg gcgtaatgtg gttcgtgctt tgtatgaatc cggacgccgg
     8881 gcggaaatta ccgtggtggc aatcaacgaa ctggcggatg ctgcgggcat ggcgcatttg
     8941 ttgaaatacg acaccagcca tggccgtttt gcatgggaag tacgacagga acgcgatcaa
     9001 ctttttgttg gtgatgacgc catccgcgta ttgcatgaac gttcactgca atcgctcccc
     9061 tggcgtgaac ttggcgttga tgtagtcctc gactgcaccg gcgtatatgg ctcccgcgag
     9121 catggcgaag cgcacattgc cgccggggct aaaaaagtgc tcttttcaca tcctggcagt
     9181 aacgatctcg acgcgaccgt tgtttacggc gtcaatcagg atcaacttcg tgcggagcac
     9241 cgcatcgttt ctaacgcttc ctgtaccacg aattgcataa ttcccgtcat caaattgtta
     9301 gatgatgcgt acggtattga gtccggcact gtgaccacaa ttcactccgc catgcacgat
     9361 caacaggtta ttgatgcata ccatcctgac ctgcgtcgca cccgggcagc cagccagtcg
     9421 atcattccgg tcgatactaa actggccgcc ggtatcacac gattttttcc gcaatttaac
     9481 gatcgctttg aagcgattgc ggtacgtgtg ccaaccataa atgtgacggc aatcgattta
     9541 agcgtgacgg tgaagaaacc tgtaaaagcc aatgaagtca acctgttgct gcaaaaagca
     9601 gcacaaggtg catttcatgg tatagttgac tatacggaat tgccgttggt ctctgtagat
     9661 tttaaccacg atccgcacag tgccattgtc gatggcaccc aaacccgggt cagtggcgca
     9721 cacctgatca aaacgttggt ctggtgcgat aacgaatggg gctttgctaa ccgaatgctc
     9781 gacacgacgt tagctatggc tactgttgct ttcaggtaag acgcaagcag cgtctgcaaa
     9841 acttttagaa tcaacgagag gattcaccat gtctgtaatt aagatgaccg atctggatct
     9901 tgctgggaaa cgtgtattta tccgtgcgga tctgaacgta ccagtaaaag acgggaaagt
     9961 aaccagcgac gcgcgtatcc gtgcttctct gccgaccatc gaactggccc tgaaacaagg
    10021 cgcaaaagtg atggtaactt cccacctggg tcgtcctacc gaaggcgagt acaacgaaga
    10081 attctctctg ctgccggttg ttaactacct gaaagacaaa ctgtctaacc cggttcgtct
    10141 ggttaaagat tacctcgacg gcgttgacgt tgctgaaggt gaactggttg ttctggaaaa
    10201 cgttcgcttc aacaaaggcg agaagaaaga cgacgaaatc ctgtccaaaa aatacgctgc
    10261 actgtgtgac gtgttcgtaa tggacgcatt cggtactgct caccgcgcgc aggcttctac
    10321 tcacggtatc ggtaaattcg ctgacgttgc gtgcgcaggc ccgctgctgg cagctgaact
    10381 ggacgcgctg ggtaaggcac tgaaagaacc tgctcgcccg atggtggcta tcgttggtgg
    10441 ttctaaagta tctaccaaac tgaccgttct ggactccctg tctaaaatcg ctgaccagct
    10501 gattgttggt ggtggtatcg ctaacacctt tatcgcggca caaggccacg atgtgggtaa
    10561 atccctgtac gaagctgacc tggttgacga agcaaaacgt ctgctgacca cctgcaacat
    10621 cccggttccg tctgatgttc gcgtagcaac cgagttctct gaaaccgctc cggctaccct
    10681 gaaatctgtt aacgacgtga aagctgacga gcagatcctg gatatcggtg atgcttccgc
    10741 tcaggaactg gctgaaatcc tgaagaatgc gaaaaccatt ctgtggaacg gtccggttgg
    10801 cgtgttcgaa ttcccgaact tccgcaaagg tactgaaatc gtggctaacg ctatcgcaga
    10861 cagcgaagcg ttctccatcg ctggcggcgg cgacactctg gcagcaatcg acctgttcgg
    10921 cattgctgac aaaatctcct acatctccac tggcggcggc gcattcctcg aattcgtgga
    10981 aggtaaagta ctgcctgcag tagcgatgct cgaagagcgc gctaagaagt aaaaaatcac
    11041 agggcaggga aacctgccct tgtttcagcg cgcttttaga gcacgcattt tttcatactc
    11101 taaataattc gagttgcagg aaggcgacaa gagagtgaat ccccaggagc ttacataagt
    11161 aagtgactgg ggtgagcgaa cgcagacgca gcacatgcaa cttgaagtat gacgagtata
    11221 aggcccgacg atacaggaca agagacatgt ctaagatttt tgatttcgta aaacctggcg
    11281 taatcactgg tgatgacgta cagaaagttt tccaggtagc aaaagaaaac aacttcgcac
    11341 tgccagcagt aaactgcgtc ggtactgact ccatcaacgc cgtactggaa actgctgcta
    11401 aagttaaagc gccggttatc gttcagttct ccaacggtgg tgcttccttt atcgctggta
    11461 aaggcgtgaa atctgacgtt ccgcagggtg ctgctatcct gggcgcgatc tctggtgcgc
    11521 atcacgttca ccagatggct gaacattatg gtgttccggt tatcctgcac actgaccact
    11581 gcgcgaagaa actgctgccg tggatcgacg gtctgctgga cgcgggtgaa aaacacttcg
    11641 cagctaccgg taagccgctg ttctcttctc acatgatcga cctgtctgaa gaatctctgc
    11701 aagagaacat cgaaatctgc tctaaatacc tggagcgcat gtccaaaatc ggcatgactc
    11761 tggaaatcga actgggttgc accggtggtg aagaagacgg cgtggacaac agccacatgg
    11821 acgcttctgc actgtacacc cagccggaag acgttgatta cgcatacacc gaactgagca
    11881 aaatcagccc gcgtttcacc atcgcagcgt ccttcggtaa cgtacacggt gtttacaagc
    11941 cgggtaacgt ggttctgact ccgaccatcc tgcgtgattc tcaggaatat gtttctaaga
    12001 aacacaacct gccgcacaac agcctgaact tcgtattcca cggtggttcc ggttctactg
    12061 ctcaggaaat caaagactcc gtaagctacg gcgtagtgaa aatgaacatc gataccgata
    12121 cccaatgggc aacctgggaa ggcgttctga actactacaa agcgaacgaa gcttatctgc
    12181 agggtcagct gggtaacccg aaaggcgaag atcagccgaa caagaaatac tacgatccgc
    12241 gcgtatggct gcgtgccggt cagacttcga tgatcgctcg tctggaaaaa gcattccagg
    12301 aactgaacgc gatcgacgtt ctgtaagtca ttcctttctg cttatctcaa ggcccgctct
    12361 gcgggtcttt ttttcgccaa aagcaaaatt gcctgatgcg ctatgcttat caggcctaca
    12421 cgtatcctgc aatatattga atttgcaggt ttttgtaggc cggataaggc gttcacgccg
    12481 catccggcat gacaaagcgc acattgatat ctgtctgagc cccgctctgc ggggattttt
    12541 ttgccaaaag caaggtgatt cagatgagaa atctgtgatc tatttggcaa aattatgctt
    12601 tattgtttac ccttgtcaga ctgcccgtca taaggcggcg gagtgtattt ctccattttg
    12661 agtcagttga aaaggaatat tgaatggaag atttgaatgt tgtcgatagc ataaacggcg
    12721 cgggaagctg gctggtagct aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg
    12781 cggcactcgc gatcatcatc gttggtttga ttatcgcgcg gatgatttcc aacgcggtga
    12841 atcgcctgat gatctcccgt aaaatcgatg ccactgttgc tgattttctt tctgcattag
    12901 tccgttacgg tattatcgcc tttacgctaa tcgcagccct gggacgcgtg ggtgtacaaa
    12961 cagcgtcagt cattgctgta ctcggtgccg caggcttagc tgttggtctg gctttgcagg
    13021 ggtcactttc taacctggcc gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg
    13081 gagaatatgt tgacctcggc ggcgtagccg gtactgtgct gagtgtgcag attttctcca
    13141 ccaccatgcg tactgcagac ggtaaaatta tcgttattcc gaacggtaaa attattgccg
    13201 gaaatattat taacttctcc cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg
    13261 cgtatgattc cgatatcgat caggttaagc agatcctgac caatattatc cagtctgaag
    13321 atcgcatttt gaaagatcgc gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga
    13381 ttaatttcgt ggtccgcgtc tggagcaaca gcggcgatct gcaaaacgtg tactgggatg
    13441 tgctggagcg tattaaacgt gaatttgatg ccgccggtat cagcttcccg tacccgcaaa
    13501 tggatgtgaa ctttaagcgg gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag
    13561 ataatactct ctggcctctc tcttattagt tattctgatt gccaattaat attatcaatt
    13621 tccgctaata acaatcccgc gatatagtct ctgcatcaga tacttaattc ggaatatcca
    13681 acgtgttttc ttattacttt caaggtcttg ctcttggggc agctatgatt ctgccgctcg
    13741 gcccacaaaa tgcttttgtg atgaatcagg gcattcgtcg tcagtaccac attatgattg
    13801 ccttactttg cgctatcagc gatttggtcc tgatttgcgc ggggattttt ggtggtagcg
    13861 cgttattgat gcagtcgccg tggttgctgg cgctggtcac ctggggcggc gtagtcttct
    13921 tgctgtggta tggttttggc gcttttaaaa cagcgatgag tagtaatatt gagttagcta
    13981 gtgccgaagt cctgaagcaa ggcagatgga aaattatcgc caccatgttg gcagtgacct
    14041 ggctgaatcc gcatgtttac ctagatactt ttgttgtact gggcagcctt ggcgggcagc
    14101 ttgacgtcga accaaaacgt tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt
    14161 tctttggtct ggctattctc gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac
    14221 agcgcattat caatctggta gtgggatgtg tcatgtggtt tatagccttg cagctggcga
    14281 gagacggtat tgctcatgca caagccttgt tcagttaggc gctatctgat ggaaaaataa
    14341 aacagaggcg ctaagcttgc ctccagagat cctgaatttt gggcaagtca attaacacgg
    14401 agagactaac gtgaagttca aagttatcgc cctggcggca ttaatgggta ttagcgggat
    14461 ggcagcgcag gctaacgaat tgccggatgg accgcatatt gtcacctccg gtacggcaag
    14521 tgtggatgcg gtgccagaca ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga
    14581 tgccgctaca gccaagaaac aggcagatga gcgcgtcgca caatacattt ccttccttga
    14641 actcaatcag atcgcgaaaa aagatatcag ctcagcgaat ttacgcaccc agccagatta
    14701 tgattatcag gatggtaaaa gtatcctgaa aggctaccgc gctgtgagaa cggtggaagt
    14761 cacgctccgt cagttagaca aactgaattc cttgctggat ggcgcgctga aggcgggtct
    14821 taacgaaatt cgttcagtgt cgctgggcgt ggcgcagccg gatgcctata aagacaaagc
    14881 gcgtaaggca gcgattgata acgcgattca tcaggcgcag gaactggcga acggctttca
    14941 tcgtaaactg gggccggtat atagcgtgcg ctaccatgtt tccaactatc agcccagccc
    15001 aatggtgcgg atgatgaaag ccgatgccgc gccggtgtct gcccaggaaa cttacgagca
    15061 ggccgctatt cagtttgatg atcaggtcga tgtggtcttc cagttagaac ctgtggatca
    15121 acaacccgct aaaacacctg cagcacaata atacttgtag cccgacgtat tcgtcgggct
    15181 aatgccttta cccgatattg cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat
    15241 cacctatctt tgctcgcggt ttcatcaagg ataggctgac tgaaacggtt aagcggacaa
    15301 ggatgcgggg aggggggatg gacattttta tttcgaaaaa gatgcgcaat ttcattttat
    15361 tagcgcaaac caataatatt gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat
    15421 ttggtaaaag cattgccgcg ctggaagaac aaattggcta tacgctattt acccgcaaag
    15481 ataataatat cagcctcaat aaagccggtc aggaactgta tcaaaaactg ttcccggttt
    15541 atcaacgact ttctgcgatt gataatgaaa tccataactc agggcgtcgt tcacgagaaa
    15601 ttgtgattgg catagataat acttatccga caattatttt cgatcagttg attagtctcg
    15661 gagataagta tgaaggcgtg accgcccagc cagttgagtt cagcgaaaac ggcgttatcg
    15721 ataatctttt tgaccgtcag ttagatttta ttatctcccc acagcatgta tctgcacgag
    15781 ttcaggaact ggaaaacctg actatcagtg aattaccacc gctgcgattg aactttctcg
    15841 tttctcgccg ttatgaagag cgtcaagagc aagagctgct tcaggagctg ccctagctac
    15901 aaatgcgctt tcaaaaccgg gctaacttcg aagcaatgat tgatgcgaat atgcgacctt
    15961 gtgggatcaa tcccaccatt atttatcgtc cctacagttt tatggccaaa atcagcgccg
    16021 tggagcgcgg acattttctg acggtgatcc cgcattttgc ctggcgactg gtgaatccgg
    16081 caacgctgaa atattttgat gcgccgcaca ggccgatgta tatgcaggaa tatctttatt
    16141 caatcagaaa tcatcgttat actgccacga tgcttcagca tattgctgaa gatcgtgacg
    16201 ggacaaacca ttaacccagc atcgagccgg ttgcaattaa attacggtgt aagtcgaaga
    16261 cgtggctaag atcgtggtga atatgtccgc cgggcgcatt ttccagatag cgatgcagat
    16321 aatcccgata cataggatgt gcacaattat caatgatagt gcgggcgcgt tgaagcgggg
    16381 aaagaccgcg cagatcggcg atcccttgtt cggtgatgat cactttgacg ctgtgttcgt
    16441 tgtgatcaac atggctgcac attggcacga cggttgagat cttgccttct ttagcaatcg
    16501 acggagccat aaagatcgac agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc
    16561 cgttcatcag atcgaccccg gctacgtgcg ttgagttggc atgcccgtaa atatcaaact
    16621 ccaggccgac gttcagagcg atgacgccca gacgacggat gatttccggg ttattggaaa
    16681 tctcctgcgg acgcaacaca atgcggctgg caaagtaatc catattgtcg taaatcttgc
    16741 gcagggaatc ggccgagatt gtcaggctgg aggcgctggc cccgctgatt ttgccggttt
    16801 ccagtaaatg caccaccgat tcctgtagca cttccgaata catcataaac ggaggaattt
    16861 ccgggttttc ccccagacgc gccattaccg cattattgat attgcccacg ccactttgca
    16921 gcggcagaaa ttccggcgga atacgcccat gcgccatttc ctgcaataag aacgtgacca
    16981 cgttatcggc aatctgctgg cacatgggat tttgcttatc cagcatatta ccggcgtcgg
    17041 gcaagttggt ttccacgacg gcgacaatct ttttcggatc gatttgcaca tagcgggtac
    17101 cgacgcgatc cattgcatgg aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa
    17161 tcacaatatc cgccagttct gcaacgcgcg gatcgtgata gtgattgagt tcaatgatca
    17221 ctttcttcgc tcgcagcagc caggtcggcg cattaccgat cccgctggtt aaccagactc
    17281 gaccatctgg tgccagtgcc gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac
    17341 cgtaattgac catttgcgcc acttcgctca aatgcaggtc aacgaaactc accgcgccct
    17401 gattgatctt tttacgtaaa ccggacgatg tttgatatgg cgcacgccag gaaacagcat
    17461 cggcgtcaga aagtacatcg tcagcggcgg cgctgattga cgcccccgtc agcaggcgaa
    17521 tttgatacgg ctttttggcc tcatgctgtt cgttagctct gcgggcaatc gcggtgggta
    17581 gggctttcgg cgaacccgct ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga
    17641 taatttctgc cgcttcatcg gcggtcatcc ttgtccactg agtttccatt tttactcctt
    17701 cgttcgcagg gattaatgac caacgaaatt aggtttacgt ttttcgagga aagcgttcat
    17761 cccttcctgg taatcttcgc tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa
    17821 ttcatcggag ttcatggtgt gtgcttcgcc cagtacacgc agctcttctt tgataacggc
    17881 aatggctaac ggcgctttct cagagatgtg gtgcgccatt tgtaaggtga aatcttccag
    17941 ttcttccact tccacaacat ggttgaggat gccgacagcc agcgcgcgct gggcggtgat
    18001 gggcgaagcg gtaaaaatca gctctttgac aatgtggaag cccgcgtcgc gggtcaggtt
    18061 gtgaatgccg accaggttat acgggacgcc gaggtttaca ggcgtcattg agaaggttga
    18121 ggtactggcg gcgataatca gatcggaact catgatcatt tcaaatgcgc caccccaaac
    18181 actaccttcc accatcgaaa taatcggttt cgggaatttt tggatcatgc gggtgatttg
    18241 acgcaatgga tcatcatagg agagcggatc gcgaccgcca gacggcagtt cgtgaatatc
    18301 gtgacctgcg gagaagactt tggatccact cggcgcgcgc aaaatgatac agcgaatttc
    18361 cggccggttg agatcgctta acgcctgcat aagatcatca ataaagactt tacttaaggc
    18421 attaagtttt cggccatagt taaactcaat gaccgccact ttgttgatag tgacaacgtt
    18481 aacatactga taagacataa aaattccttt aatcaaaata ttgcgtctgg ataaattcac
    18541 tgagctgccg caggccggtg cgcggtgaga gcgtattgtt tttgaccgct aaaagcgtct
    18601 ggcggtaata gcgatcgaaa tcttcattcg cgaacaggtg attcagtact tcttcttcgg
    18661 tctgcttacg cagccattcc accgattgtt gttgccgcac ttgttgtaaa cgaccactgg
    18721 cagttagcgc ggttttgaag tcgatgatgg cgtgccagat ctcatcgatt ccacgttttt
    18781 ccagtgcgct acaagtcaga acccgtggct gccattcgtc gtatttacgt cgcagaatat
    18841 gcagggcact ctcgtacata tgccgggcaa tggcgacatt ggtatggtta tcgccatcgt
    18901 ctttgttgat aacgatcaga tcagccactt ccatcagccc ttttttaatg ccctgcagat
    18961 catcgccgcc accggcaatt tgcaacgaga taaaacagtc caccatgcgg gcgacttctg
    19021 tttccgactg cccgacgcca accgtttcga caatcactac gtcataaccc gctgcttcgc
    19081 ataacagcat taattcccgc gctcgctgac tggcaccgcc cagatgaccg gaggatggta
    19141 ccgggcgaat aaacgccgct tcggcacgcg ccaggtcatt catgcgggtt ttatccccga
    19201 gaatgctacc gccagtgacc gggctgctgg gatcgaccgc aataaccgcg acctttaatc
    19261 cctctcgaat caacaacatg ccaaaggcct caagaaaggt acttttcccc gcgccggggg
    19321 tgccggtaac gcccagtcgc agggtgttac cgcagtacgg cataatggca tcaagcagct
    19381 gcgtacttag tgcctgatga cgcgggtgac ggctttccac cagcgtcatg gcctgggcga
    19441 gtgtggcatg ctcaccctga cgtaagcggc gaatactttc tgccagcgtg gcttcattaa
    19501 tcatgatgct ggcttatcag attcagtacg tcgcgcacac tgtcgagcat aggtgtacct
    19561 ggaccataaa tcgccgccac gccgcgctct tgcaggaagg cgtaatcctg cggtggaatg
    19621 acgccaccgg cgactacgca gatatcttcg cgtccccatt ttttcagcgc ttcgaccagt
    19681 tccgggatca gcgttttatg accggcagcc agtgaggatg cgcccactac gtgaacgtca
    19741 ttttctactg ccaggcgggc gatctcttca ggtgtagaga acatcgggct taaatctacg
    19801 tcgaaaccga gatcggaata ggcgctggcg atcactttcg cgccgcgatc gtgtccatcc
    19861 aggcccattt tggcgatcag aatgcgcggg cgacgaccat tgtcggcaag gaactgctcc
    19921 gtttgcgcaa caatggcatc gaactcggag gccgatttct cagattgatg atagctttgc
    19981 gcaatcacgc cggtaacaca ctggcttggc accagataac ggtcgaaagc ggcttccagc
    20041 gcatcggaaa tttcacccag ggtggcgcga acgcgagcgg cattaacagc ggcagccagc
    20101 aggttttcgt tatgctgtgc ggcgtgagtc agggcgttca acgcggcggt tacggcggca
    20161 tcatcacggg tggcgcgaat gcgttccagc gaagcaattt gctcgttacg caccatcacg
    20221 ttgtcgatct caagtacatc ggtttcgtct tcgtgatcca gtttgtactt gttgacacca
    20281 acgatgacac gcttgccctg gtcgatcagc gactgttcgc gcgctgaggc ctcttcgatc
    20341 attcgttttg gcagacctgc ttcgatcgct ttcgccatgc caccggcttc gtcgatctgt
    20401 tggataatag ctctggcttg tttgacgatt tgatcggtca gcgactcaat gtaataggat
    20461 ccggccagtg gatcgacggt gcggcagagt tctgattctt cctggatgat gatctgggtg
    20521 ttgcgggcaa tgcgtgctga gaaatcggta ggcaaaccaa gcgcttcgtc aaaggcgttg
    20581 gtatgcagtg actgagtacc gcccagcgtc gcagccagcg cttcaatggt ggtgcggata
    20641 acgttgttat acggatcctg ttcagtcagg ctccagcctg aggtctggca gtgggtacgc
    20701 agcgccagtg atttcgggtc ctgtgcgcca aatccactga ccgcttcgct ccataaataa
    20761 cgtgccgcac gcaacatggc gacgttcata aacagatcca tgccgatgcc gaagaagaac
    20821 gacaggcgag gagcgaagtc atcaattttc agtccggcag agattgctgc tttgatgtac
    20881 tcaatcccat cagcgagcgt aaatgctacc tgctgcacgc agttggcacc cgcttcaccc
    20941 atgtggtaac cgctgatact gatggtatta aatcgcggca tgttgccgga acaccaggcg
    21001 atgatgtcgg cgataatgcg cattgacggt tttggcgggt aaatataggt gttgcggcag
    21061 aggtactctt tgagaatatc gttttgaatg gtgccggtca gtttatcagg tgtaacacct
    21121 tgctcttccg cggcgatgat ataaaacgcc agtactggta gcactgcgcc attcatggtc
    21181 atcgaaaccg acattttatc cagcgggatc tggtcgaaca ggactttcat atcttccacg
    21241 gtgtcgatag cgacgcccgt tttgccgacg tcacccgcca cgcgtgggtt atcggagtcg
    21301 tagccacggt gggtggcaag gtcaaacgca acggaaagac ctttttgccc ggcggccagg
    21361 ttacggcgat aaaaagcgtt ggactctttt gctgttgaaa aaccagcata ctgacggatg
    21421 gtccacggtt gggcggtata catagtggca cgcgggccac gaacgtaggg cggcaaacca
    21481 ggaagggtac ctgtcacctc cagattatcg agatcggctt cggtatacag cggcttgatg
    21541 gcgatccctt ccgcggtttg ctgaaccagc gagtcgacag ttttctcccg acggctcaat
    21601 tccttgttgg caagctgttg ccactcctgc tcgttagaca ttatttgctc cgtaaaacgc
    21661 aatcaaaatg agggtatttg cgcttacggt gagcttttta cgtgggtttg tcgccagcaa
    21721 aaaaaatgca ttcagacgtc gttttttgcc gcctgagatt tgtctatttt gtgatgtgat
    21781 tcaaggaata caaaaagaag tgaggataat gcctgatatg tgctatcagg cattatttga
    21841 tggattaatc ctgacgaagg actttgtgac cataatcgag caacgcatca gtgactttac
    21901 gcatcatgcg gctttcagga gcaaagcggt gccagtagag catccggcgt tgaaatagcc
    21961 ctggcgttaa gtcaatcagt tcaccgctgg ctagttcttt ctcgatttgc aggtgcggga
    22021 tcatacagca ggtggtgccc tggcgagcaa gttgcacgaa cgcttctgaa gaattaacga
    22081 tatggcaggg cacgctgcct ggaggcagat cgaagttttg ctgcaaaaag gcctggtgca
    22141 tatcgtcaag atggtcaaac gcgaccactg gcgctttcag taatgccgaa cgcgttacgc
    22201 cgttagggaa atatttttcg gcaaagggtt ttgagctgac gaacagatag tcgagcgcac
    22261 caagtttatc gacaagacaa ctcggcagcg cctgatgttg aatactcacc gcgccgacca
    22321 cttcgccgcg acgcagacgt tcctgagtgc gggtttcatc ttctacctgc aagttgaggc
    22381 ggataggcga atcagccaat acaggagcca gtgccggaag caaccacgtc gccagactgt
    22441 cggcattgac cgccagtgaa agcagcagcg gagtcgaacc ggtttgttca tcgcccagcc
    22501 actcttcttc cagcaactcc acctggcgca gcagtgccag cagtttttgc ccttgttccg
    22561 tcgggcgcgg cggtacggta cgcaccaaca gcggctgccc gaacatattt tccagttgct
    22621 taatgcgctg tgagacggct gattgtgtaa tgcacagctt ttgtgccgcg cgctcaaatc
    22681 ctcgttcacg tatcaccgca tccagtgcct gtaatgttct gtagtccggg cgtttcattg
    22741 ctgttgtctg ctcctggtaa atgctttttc ctgcactatg acacaatttt gtgtcaggtt
    22801 gcatataccg cagccgtcgg cgggtaacat ttgtatcaat ggttaaacgc gcagcttaaa
    22861 agatcaggga tttgcgctat tgcatttcgc ttagcaactt cgcgccctgg ttatgttgaa
    22921 tggcgtggcg ttattgcctc tgttcgcacc gtggagcggc atacccagac cgttgtatgc
    22981 tttatttgcg gggcgaaagg ggatgcccgc cattgcgcga tattttatgg atgagttaac
    23041 cacgcggctt gccaacgggg tctgaatcgc tttttttgta tataatgcgt gtgaaatttc
    23101 ataccacagg cgaaacgatc atgacgcagg atgaattgaa aaaagcagta ggatgggcgg
    23161 cacttcagta tgttcagccc ggcaccattg ttggtgtagg tacaggttcc accgccgcac
    23221 actttattga cgcgctcggt acaatgaaag gccagattga aggggccgtt tccagttcag
    23281 atgcttccac tgaaaaactg aaaagcctcg gcattcacgt ttttgatctc aacgaagtcg
    23341 acagccttgg catctacgtt gatggcgcag atgaaatcaa cggccacatg caaatgatca
    23401 aaggcggcgg cgcggcgctg acccgtgaaa aaatcattgc ttcggttgca gaaaaattta
    23461 tctgtattgc agacgcttcc aagcaggttg atattctggg taaattcccg ctgccagtag
    23521 aagttatccc gatggcacgt agtgcagtgg cgcgccagct ggtgaaactg ggcggtcgtc
    23581 cggaataccg tcagggcgta gtgaccgata acggcaacgt gatcctcgac gtccacggca
    23641 tggaaatcct tgacccgata gcgatggaaa acgccataaa tgcgattcct ggcgtggtga
    23701 ctgttggctt gtttgctaac cgtggcgcgg acgttgcgct gattggcaca cctgacggtg
    23761 tcaaaaccat tgtgaaatga tctgacgggg gaacctcccc cgttaaaaaa attctcttca
    23821 ttaaatttgg tgacatgtgt cacgttttta cctggatatt gtcgattgct ctaaataaat
    23881 cctctaaacc agcatattca accaagaaat acctttgcgt gatatttcct caacatcgcg
    23941 acgcaaacgt tcatattgcc gcaatattat tttttgatat gttgaaaggc ggatgcaaat
    24001 ccgcacacaa catttcaaag acaggattgg gtaaatggca aaggtatcgc tggagaaaga
    24061 caagattaag tttctgctgg tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc
    24121 agctggttac accaacatcg aatttcacaa aggcgcgctg gatgatgaac aattaaaaga
    24181 atccatccgc gatgcccact tcatcggcct gcgatcccgt acccatctga ctgaagacgt
    24241 gatcaacgcc gcagaaaagc tggtcgctat tggctgtttc tgtataggaa caaaccaggt
    24301 tgatctggat gcggcggcaa agcgcgggat cccggtattt aacgcaccgt tctcaaatac
    24361 gcgctctgtt gcggagctgg tgattggcga actgctgctg ctattgcgcg gcgtgccgga
    24421 agccaatgct aaagcgcacc gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc
    24481 gcgcggcaaa aagctgggta tcatcggcta cggtcatatt ggtacgcaat tgggcattct
    24541 ggctgaatcg ctgggaatgt atgtttactt ttatgatatt gaaaacaaac tgccgctggg
    24601 caacgccact caggtacagc atctttctga cctgctgaat atgagcgatg tggtgagtct
    24661 gcatgtacca gagaatccgt ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat
    24721 gaagcccggc tcgctgctga ttaatgcctc gcgcggtact gtggtggata ttccggcgct
    24781 gtgtgatgcg ctggcgagca aacatctggc gggggcggca atcgacgtat tcccgacgga
    24841 accggcgacc aatagcgatc catttacctc tccactgtgt gaattcgaca acgtccttct
    24901 gacgccacac attggcggtt cgactcagga agcgcaggag aatatcggcc tggaagttgc
    24961 gggtaaattg atcaagtatt ctgacaatgg ctcaacgctc tctgcggtga acttcccgga
    25021 agtctcgctg ccactgcatg gtggtcgtcg tctgatgcac atccacgaaa accgtccggg
    25081 cgtgctaact gcgctgaaca aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata
    25141 tctacaaact tccgcccaga tgggttatgt agttattgat attgaagctg acgaagacgt
    25201 tgccgaaaaa gcgctgcagg caatgaaagc tattccgggt accattcgcg cccgtctgct
    25261 gtactaattc cccttctctg aaaattaacg ggcaggtcac tgacttgccc gtttttttat
    25321 cccttctcca caccgttgcc gcgttgcgca ttctactttg cgagtctgct ccagaaaata
    25381 aatcaaagtc agatcaatgc gttgtgttta tttaatgggg atttacgatg gcgggacagc
    25441 atggggctgt aacggtgaag ccctcaccga agcgagggct taaaggagaa gggttatgat
    25501 gcgacttgtc atcatactga ttgtactgtt actgataagt ttcggagctt attaacagtc
    25561 agtctcaggg gaggagtcat cctcccttac ccttactcac taaattaggt caaagaatca
    25621 acgatgtcaa tcagggcgat gcggggtgca tcgccctgaa ccgaactttt tgatagccac
    25681 ttcatcgccc acgtttactg ccgtaattaa tcccttattc ctgcaatgta tgtctgcctt
    25741 acatgacgtt ttacgagtgc gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg
    25801 ttatttaaag agagtttacg atggcagggc agcatggggc tgtaacggta aagccctcac
    25861 cgaagcgagg gcttgaagga gaagggttat gatgcgactt gtcatcatac tgattgtact
    25921 gttactgata agtttgccag cttattaaca gtcagtctca ggggaggagt catcctccct
    25981 tacccttact cactaaatta ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg
    26041 tatcgccctt accactccca gactttcgac ggtgtaacca ccgcaggaag agggatatcc
    26101 cactcttcaa cggggagttt ttccaccaac tgacaatcat gcgcataacc caccggttgc
    26161 gttttatagt gctgccagtt ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg
    26221 cgctgaccgt actcatcaaa ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg
    26281 gggagcacgt cacgcacatc cagttttggc tcatggattt tcaacctgtt catcaccagt
    26341 tcgctttgcg gatggtaatt caggaacagc aaattaccgg cactaaaggg atgcaaaact
    26401 ggaagatata cgcgcttacc ggcgcgccag agttgttcta taagcggctg ggtgtcgagt
    26461 tcaccatcaa aagagaggaa tacagcgacc gtatgcgcca tcaccaccgg gggataagtc
    26521 atcatccggg tagcggcttg ttgacccatt tcctgctgtt gttccggcgt taacgcacga
    26581 cgacgttgcc gaatcatttt gcggatttct tgtcgggata atgtcagtgg gagttctggt
    26641 agttgcgtca tggctggtgc cagataagaa gggaatctcc gagatgccgc cgcaggctgt
    26701 aacccttgaa cccttggttc aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg
    26761 gaccgagcat gctcaccaac cgcggagcgc cacattcttg tggtatgaaa tatcggctca
    26821 ggggactggc ccgcttgcga acatctcaga gaaattttgt cttcacggtt actctaccac
    26881 agtaaaccga aaagtgtcat tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt
    26941 caagtaacgc ttgttctatg gtctgctgca gcatccgaat acgctgttcc atacttgccg
    27001 cgtagtcacg agtcttcgct ttttcttgcg ctaactcata gctgatattc aatgcggcaa
    27061 tgaagaccaa ctgttcagta tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt
    27121 ggttcagatc gtccgctgcc tgattcaacg catccctttg gtcaggcggg cagttcacac
    27181 gcagtgaacg gccaaaaatt tggatatcga cgggttgtgc agacatgcca ccttcctgct
    27241 gattgactgc gctgccttcg tctttagacc catggtctgc gaaggggcga cactatagct
    27301 accctgatga gaagagacaa gcccttttct ggtccaccag ggcccaaagt ggtagcatat
    27361 catgaatatt cctccctttg acgacgaatg cttatgtcta tacagaacga aatgcctggt
    27421 tacaacgaaa tgaaccagta tctgaaccaa caagggacgg gtctgacccc tgctgagatg
    27481 catggtttaa tcagtgggat gatatgtggc ggtaacgatg acagctcatg gctaccgcta
    27541 cttcacgacc tgacgaacga aggcatggct ttcggtcatg agctggcaca ggcattgcgt
    27601 aaaatgcact ctgccaccag cgatgccctg caggatgacg gcttcctttt tcagctttat
    27661 ctgcctgatg gcgatgatgt cagcgttttc gatcgggctg atgcattggc aggttgggtc
    27721 aatcacttcc tgcttggtct tggtgttacg caaccgaagc tggataaagt gaccggcgaa
    27781 accggtgaag ctatcgacga tctgcgtaac attgcgcaac tgggttacga cgaagacgaa
    27841 gatcaggaag agcttgaaat gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg
    27901 ctgttatgcc acgacacctt tactcatccg caaccgaccg cgccagaagt acaaaaaccg
    27961 actctacact aaaaacaaaa aacgtaagga gagtgttatg agtgagatat cccggcaaga
    28021 gtttcagcgt cgccgtcagg ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat
    28081 ttttgctgca ccagaagtaa cacgtagcgc cgacagcgaa tacccctatc gtcagaacag
    28141 tgacttctgg tacttcaccg gctttaacga accggaagcg gtgttggtgc tgattaaaag
    28201 cgatgacact cataaccaca gcgttctgtt taaccgcgtt cgcgacctga cggcggagat
    28261 ctggtttggc cgtcgcttag gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc
    28321 actggcattc agcgaaatta atcagcaact ttatcaacta cttaatggcc tggatgtggt
    28381 ttaccatgct cagggcgaat atgcatatgc tgatgaaatc gtgaacagtg cgctggaaaa
    28441 actgcgtaaa ggttcgcggc aaaatctcac cgcaccggca acgatgatcg actggcgtcc
    28501 tgttgttcat gaaatgcgcc tgttcaaatc gccagaagag attgccgtac tccgccgcgc
    28561 gggagaaatc accgccatgg cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt
    28621 cgagtaccat ctggaaggcg aaattcacca cgaatttaac cgccacggtg cgcgctatcc
    28681 gtcctataac accattgtag gcagcggtga aaacggctgc attttacact acaccgaaaa
    28741 cgagtgtgaa atgcgcgacg gcgacctggt gttgattgac gcaggctgcg aatacaaagg
    28801 ttacgcaggc gatatcaccc gtaccttccc ggtcaacggc aaattcaccc aggcccagcg
    28861 cgaaatctac gacattgtgc tggagtctct tgaaaccagc ctgcgcctgt atcgtccggg
    28921 aacctccatt ctggaagtca ccggtgaagt ggtgcgcatt atggttagcg gcctggtaaa
    28981 actcggcatc ctgaaaggtg atattgatga actgatcgct cagaacgccc atcgtccttt
    29041 ctttatgcat ggccttagcc actggttagg actggatgtc catgacgttg gcgtttatgg
    29101 tcaggatcgc tcgcgcattc tggaaccggg gatggtactg accgtagagc ctggtctgta
    29161 tatcgcgccg gatgcggatg tgccagaaca atatcgcggt atcggcattc gtattgaaga
    29221 cgacattgtg attaccgaaa ccggcaacga aaacctcacc gccagcgtgg tgaaaaagcc
    29281 ggaagaaatc gaagcgttga tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt
    29341 ggcggcatgg cgggcgcgac gctggcgctg gctatttccc ggttaagtca cggggcgctg
    29401 ccggtacatt tgattgaagc gactgcgcca gagtcacatg ctcatccggg ctttgatgga
    29461 cgagcgatag cgctggcggc gggtacctgt cagcaactgg cgcgcatcgg cgtttggcaa
    29521 tctctggcgg attgcgcaac tgccatcacc accgtgcatg tcagcgatcg aggtcacgct
    29581 ggatttgtca ccctcgccgc agaagattac caactggcgg cgctgggaca ggttgtcgaa
    29641 ttgcacaatg tcgggcaacg gctgtttgca ttgctgcgta aagcacctgg cgtaacgctg
    29701 cattgccctg atcgcgtggc taacgttgcc cgtactcaga gtcacgttga agtgacgctg
    29761 gagggtggcg agacgctgac gggccgcgtg ctggtagccg ctgatggcac ccattcagcg
    29821 ttagccactg tgtgcggcgt tgactggcag caggagcctt acgaacaact ggctgtgatt
    29881 gccaacgttg cgacttccgt tgcgcatgaa gggcgcgctt ttgaacgctt tacgcaacat
    29941 ggcccgctgg cgatgttgcc gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca
    30001 ctggaacggc gcgaagaggt gctgtcgtgg agcgacgaga agttttgccg tgaactccag
    30061 tcggcctttg gctggcgact tgggaaaatt acccacgctg gtaaacgcag tgcttatccg
    30121 ctggcgttaa cccacgccgc cagatctatt acccatcgta ccgtgctggt gggcaatgcg
    30181 gcgcaaaccc tgcacccgat tgccgggcaa gggttcaacc tcggtatgcg agatgtcatg
    30241 agtcttgcgg aaaccctgac tcaggcgcag gagcgcggag aagacatggg tgattacggc
    30301 gtattgtgtc gttatcagca gcgtcgacag agcgatcgcg aagcaaccat tggcgtcacg
    30361 gacagccttg tacatctttt tgccaaccgt tggacgccac tggttgtcgg gcgcaacatc
    30421 gggctgatga cgatggaatt attcaccccg gcacgcgatg tgctggcgca gcgcaccctc
    30481 ggttgggtgg cgcgttgaag gcggttatta aggaataaac aatgcaaagt gttgatgtag
    30541 ccattgttgg cggcggcatg gtggggctgg cggttgcctg cggcttacag ggtagcggct
    30601 tacgcgttgc cgtactggag cagcgcgtac cggaacctct ggcggcggat gcaccaccac
    30661 aactgcgcgt ttcggctatc aatgccgcca gcgaaaaatt actcacccgt cttggcgtct
    30721 ggcaggacat tctctctcgc cgagccagct gttatcacgg tatggaagtg tgggacaaag
    30781 acagttttgg tcacatctcg tttgacgatc aaagcatggg ctatagccat cttgggcaca
    30841 tcgttgaaaa ctcagtgatt cactacgcgc tgtggaacaa agcgcagcag tcgtcagata
    30901 tcactctttt ggcccccgca gaattacagc aggtcgcctg gggagaaaat gaaaccttcc
    30961 tgacgctgaa agatggcagt atgttaacgg cgcgtctggt gattggcgcg gacggcgcta
    31021 attcctggtt gcgcaacaaa gccgatattc cgctgacttt ctgggattat cagcatcacg
    31081 cgctggtagc gaccatacgc acggaagaac cgcatgatgc ggtggcgcgg caggttttcc
    31141 atggcgaagg cattctggcc tttttaccgc ttagcgatcc gcatctttgc tcgattgtct
    31201 ggtcactgtc gccagaggaa gcgcagcgga tgcagcaggc aagtgaagac gaatttaatc
    31261 gcgcgttaaa tatcgctttt gataatcgcc tgggcttatg caaggttgag agcgcgcgtc
    31321 aggtgttccc actgacgggg cgttatgcgc gccagtttgc cgcgcaccgt ctggcgctgg
    31381 tgggcgacgc cgcgcatacc attcacccgc tggcggggca gggggtaaat cttggcttta
    31441 tggatgctgc agagctgatt gctgaactga aacggttgca tcgtcagggt aaagacatcg
    31501 ggcagtacat ttatctgcgt cgctatgagc gtagccgcaa gcacagtgcg gcgctgatgc
    31561 tggctggtat gcagggattc cgcgatctgt tttctggtac caatccggtg aaaaaactgc
    31621 tgcgtgatat tggtctgaaa ctggccgaca cgcttcctgg cgttaaacca caacttatcc
    31681 gccaggcaat gggattaaac gatttgcctg aatggctgcg ttaaaaattt ctcttctgct
    31741 gtttatttga tacccatcac actttcatct cccggttttt tcgccgggag attttcctca
    31801 tttgaaataa actaatttca cctccgtttt cgcattatat tttctaatgt cacgaatttt
    31861 tgatttagtg ttttttgaca ttttttcagc tcttaatatt gccttattca aattaacttt
    31921 ctcatcacat catctttata tagaaacttg tgtatttttt ggttttttat tctgttgcga
    31981 ttttggcgtt ttttaaccat aagctaatgt gatgatcaat tttaccttat ggttaacagt
    32041 ctgtttcggt ggtaagttca ggcaaaagag aacgattgcg ttggggaccg ggagtggctc
    32101 cgatgctggg tttcgtggtg ataatttcac catgaaaaag ttgtcagccc cgcttattca
    32161 atgaggacaa gatggcacaa cagactcctt tgtacgaaca acacacgctt tgcggcgctc
    32221 gcatggtgga tttccacggc tggatgatgc cgctgcatta cggttcgcaa atcgacgaac
    32281 atcatgaggt acgtaccgat gccggaatgt ttgatgtgtc acatatgacc atcgtcgatc
    32341 ttcgcggcag ccgcacccgg gagtttctgc gttatctgct ggcgaacgat gtggcgaagc
    32401 tcaccaaaag cggcaaagcc ctttactcgg ggatgttgaa tgcctctggc ggtgtgatag
    32461 atgacctcat cgtctactac tttactgaag atttcttccg cctcgttgtt aactccgcca
    32521 cccgcgaaaa agacctctcc tggattaccc aacacgctga acctttcggc atcgaaatta
    32581 ccgttcgtga tgacctttcc atgatcgccg tacaagggcc gaatgcgcag gcaaaagctg
    32641 ccacactgtt taatgatgct cagcgtcagg cggtggaagg gatgaaaccg ttctttggcg
    32701 tgcaggcggg cgatctgttt attgccacca ctggttatac cggtgaagcg ggctatgaaa
    32761 ttgcgctgcc caatgaaaaa gcggccgatt tctggcgtgc gctggtggaa gggggcgtta
    32821 agccatgcgg tctgggcgcg cgtgacacgc tgcgtctgga agcgggcatg aatctttatg
    32881 gtcaggagat ggacgaaacc atctctccgt tagccgccaa catgggctgg actatcgcct
    32941 gggaaccggc agatcgtgac ttcatcggtc gtgaagccct ggaagtgcag cgtgagcatg
    33001 gcacagaaaa actggttggt ctggtgatga ccgaaaaagg cgtgctgcgt aatgaactgc
    33061 cggtacgttt taccgatgcg cagggcaacc agcatgaagg cattatcacc agcgggactt
    33121 tctccccgac gctgggttac agcattgcgc tggcacgcgt gccgaaaggt attggcgaaa
    33181 cggcgattgt gcaaattcgc aaccgtgaaa tgccggttaa agtgacaaaa cctgtttttg
    33241 tgcgtaacgg caaagccgtc gcgtgattta cttttttgga gattgattga tgagcaacgt
    33301 accagcagaa ctgaaataca gcaaagaaca cgaatggctg cgtaaagaag ccgacggcac
    33361 ttacaccgtt ggtattaccg aacatgctca ggagctgtta ggcgatatgg tgtttgttga
    33421 cctgccggaa gtgggcgcaa cggttagcgc gggcgatgac tgcgcggttg ccgaatcagt
    33481 aaaagcggcg tcagacattt atgcgccagt aagcggtgaa atcgtggcgg taaacgacgc
    33541 actgagcgac tccccggaac tggtgaacag cgaaccgtat gcaggtggct ggatctttaa
    33601 aatcaaagcc agcgatgaaa gcgaactgga atcactgctg gatgcgaccg catacgaagc
    33661 attgttagaa gacgagtaac ggctttattc ctcttctgcg ggagaggatc agggtgagga
    33721 aatttacacc tcaccctcac tctcttcgca aggagagagg ttcacaattc actgcacgtt
    33781 tcaggaacca tcgctcatga cacagacgtt aagccagctt gaaaatagcg gcgcttttat
    33841 tgaacgccat atcggaccgg acgccgcgca acagcaagaa atgctgaatg ccgttggcgc
    33901 acaatcgtta aacgcgctga ccggccagat tgtaccgaaa gatattcagc ttgcgacccc
    33961 gccgcaggtt ggcgcaccgg cgaccgaata cgccgcgctg gcggaactca aggctattgc
    34021 cagtcgcaat aaacgcttca cgtcttacat cggcatgggt tacaccgccg tgcagttacc
    34081 gccggttatc ctgcgtaaca tgctggaaaa tccgggctgg tataccgcgt atactccgta
    34141 tcaacctgaa gtctcgcagg gccgccttga ggcactgctc aatttccagc aggtaacgct
    34201 ggatttaacc gggctggata tggcctctgc ttctcttctt gacgaagcca ccgctgccgc
    34261 cgaagcaatg gcgatggcga aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt
    34321 ggcttccgat gtgcatccgc aaacgctgga tgtggtccgt actcgtgccg aaacctttgg
    34381 ttttgaagtg attgtcgatg atgcgcaaaa agtgctcgac catcaggaca tcttcggcgt
    34441 gctgttacag caggttggca ctaccggtga aattcacgac tacactgcgc ttatcagcga
    34501 actgaaatca cgcaaaattg tggtcagcgt tgccgctgat attatggcgc tggtgctgtt
    34561 aactgcgccg ggtaaacagg gcgcggatat tgtttttggt tcggcgcaac gctttggcgt
    34621 accgatgggt tacggtggcc cacacgcggc attctttgcg gcgaaagacg aatacaaacg
    34681 ctcaatgcca ggccgtatta tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg
    34741 catggcgatg cagactcgcg agcaacatat tcgtcgtgag aaagcgaact ccaacatttg
    34801 tacttctcag gtgctgctgg caaacatcgc cagcctgtat gccgtttatc acggcccgat
    34861 tggcctgaaa cgtatcgcta accgcattca ccgtctgacc gatatcctgg cggcaggcct
    34921 gcaacaaaaa ggtctgaagc tgcgccatgc gcactatttc gacactctgt gtgtggaagt
    34981 ggccgacaag gcgggcgtac tggcgcgtgc cgaagcggct gaaatcaacc tgcgtagcga
    35041 tatcctgaac gcggttggga tcacccttga tgaaaccacc acgcgcgaaa acgtgatgca
    35101 gcttttcagc gtactactgg gggataacca cgggctggac atcgacacgc tggacaaaga
    35161 cgtggctcac gacagccgct ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac
    35221 ccatccggtg tttaatcgct accacagcga aaccgaaatg atgcgctata tgcactcgct
    35281 ggagcgtaaa gatctggcgc tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa
    35341 actgaacgcc gccgccgaga tgatcccaat cacctggccg gaatttgccg aactgcaccc
    35401 gttctgcccg ccggagcagg ccgaaggtta tcagcagatg atcgcgcagc tggctgactg
    35461 gctggtgaaa ctgaccggtt acgacgccgt ttgtatgcag ccgaactctg gcgcacaggg
    35521 cgaatacgcg ggcctgctgg cgattcgtca ttatcatgaa agccgcaacg aagggcatcg
    35581 cgatatctgc ctgatcccgg cttctgcgca cggcactaac cccgcttctg cacatatggc
    35641 aggaatgcag gtggtggttg tggcgtgtga taaaaacgga aatatcgatc tgactgatct
    35701 gcgcgcgaaa gccgaacagg caggtgataa tctctcctgc atcatggtga cctatccttc
    35761 cacccacggt gtgtatgaag aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg
    35821 cggtcaggtt taccttgatg gcgcgaacat gaacgcccag gttggcatca cctcgccggg
    35881 ctttattggc gcagacgttt cacaccttaa cctgcataaa actttctgca ttccgcacgg
    35941 cggtggtggt ccgggtatgg gaccgatcgg cgtgaaagcg catctggctc cgtttgtacc
    36001 gggtcatagc gtggtgcaaa tcgaaggcat gttaacccgt cagggcgcgg tttctgcggc
    36061 accgttcggt agcgcctcta tcctgccaat cagctggatg tacatccgca tgatgggcgc
    36121 agaagggctg aaaaaagcaa gccaggtggc aatcctcaac gccaactata ttgccagccg
    36181 cctgcaggat gccttcccgg tgctgtatac cggtcgtgac ggtcgcgtgg cgcacgaatg
    36241 tattctcgat attcgtccgt tgaaagaaga aaccggcatc agcgagctgg atattgccaa
    36301 gcgcctgatc gactacggtt tccacgcgcc gacgatgtcg ttcccggtgg caggtacgct
    36361 gatggttgaa ccgactgaat ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat
    36421 gctggctatc cgcgcagaaa ttgaccaggt gaaagccggt gtctggccgc tggaagataa
    36481 cccgctggtg aacgcgccgc acattcagag tgaactggtc gccgagtggg cgcatccgta
    36541 cagccgtgaa gttgcggtat tcccggcagg cgtggcagac aaatactggc cgacagtgaa
    36601 acgtttggat gatgtttacg gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga
    36661 ataccagtaa ttcactgatt cgactatctt ctaaaggcgc ttcggcgcct ttttagattg
    36721 ttaataaagt gcgctttgtg tgtttcggct gaggcgtaat ataaaatatt gcgagctcaa
    36781 tatattaaaa gagtgccagg cctgatgagc gtagcgcatc aggcacatcg gctaattaca
    36841 gattctcgcc gttgctggca atcacttctt tgtaccagtt aaagctcttc ttacgtgaac
    36901 gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg tagcgtttgc
    36961 tgtactgccc ggtagtgaac gacacgcagt cgatgcagcc ccacggtgtg tagcccatca
    37021 gatccacgcc atcgtaagtt actgcttttt tcatctcttc aatatgggcg cgcaggtagt
    37081 caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa gcgccaaaac
    37141 cgttttcgac aataaacagc ggcttctggt aacgctcata cagttcgcaa agtgcgtaac
    37201 gcagacccac cggatcaatc tgccagcccc agtcagatgc tttaacatac gggtttggta
    37261 cgctgccttc aaaaccagag atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg
    37321 tcatgtaata gctgaaacca agataatcgc aggtgccttc gcgcagtaca tcgagatcgc
    37381 tatcttccat tttgatgtta aatccgcgac gctcccactc gttcaacaca tacgacgggt
    37441 aatagccgcg tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga
    37501 acatcacatc gtccgggtta catgagtagg gatagagcgg caccatcgcc agcatacagc
    37561 cgactttcat ctccaggtta atgcgacgcg cggctttcac tgccagggcg ctggcgacaa
    37621 actggtgatg cagcacctga tacatcgtct cttccgggtt ttcatgctcg gtatacacca
    37681 cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg ttaatttcgt
    37741 tgaaggtcat ccagtatttg actttgtgct tatagcgttc aaataccact tccgcgaaac
    37801 gtacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc accagatgca
    37861 gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc agcagttcat
    37921 cgaacagaga gtcgtagaac ttcagccctt cttcgtttgg ctgagcttca tcgccttttg
    37981 gaaaaatgcg cgtccaggca atggatgtac gaaaacattt gaagcccatt tcggcaaata
    38041 gcttgatgtc ttccttatag tgaccataaa aatcaacggc ttcatggttt ggatagtatt
    38101 tgcctggcaa gacttctttg gttatttcgc gcggcacgcc gtgtgcgcca ccggtcagaa
    38161 cgtcacaaat gctcggccct tttccgcctt tgttccagcc gccttcgacc tgatgagcgg
    38221 caactgcgcc gccccataag aaatctttcg gtaaggtgag ttttttcact atcataggct
    38281 ccagatgagt caattcttac aggaaatagt accatgcagc caaacgacat cacttttttt
    38341 caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagtct
    38401 gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga tgacggttat
    38461 ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacgct gacggaaaaa
    38521 catgcagagc aggaaaatat gaccctgact gaactgaaaa aggtcattgc cgacatctat
    38581 cccggccaga cacagtttta tgtgattgaa tttaaatgcc tttaaattga actcacgcct
    38641 gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc aaatcagaat
    38701 ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat
    38761 gtgcgtgtac gtttctccgg agtaagttat ggttcagaag cccctcatta agcagggata
    38821 ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggttgg tgtttggtat
    38881 cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg ccacagcgat
    38941 aaccagctac agcctctatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta
    39001 tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat
    39061 ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt
    39121 agcgcgcggg ttgatgattg ttatctggag cctggcattg ctggggattc tgttcaaact
    39181 gaccatcgcg caccgattca aaattttatc tctggtgact tatctggcga tgggctggct
    39241 gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttact
    39301 ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca aacgcattcc
    39361 atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc actttctggc
    39421 gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg
    39481 ccagcgtcgc atcaggcgat aaatgcctga tgcgacacag agatttactc ttcgagcgaa
    39541 tacggcagtg gctcgataca caatgtattc gcatcgtcgc gcacgcggaa gatgctgtcc
    39601 ggttccatat cgttattcat cactacctgc accacgacct gaccatcttc cagttttacc
    39661 gcagccagca ccgtaccagt acgacgccag ttctcgccca ttttcagctc taagtcttca
    39721 ccagcttccg gcagtcggct ggcactacct gtcagcaacc agagcgcgcg tttgttggca
    39781 ccacggaatt ttgctcgcgc caccatctct tgtccggtat agcagccttt cttaaagctg
    39841 ataccgccca gcgcctggag gttggtcgcc tgtgggataa actgcccgct gttcgcggca
    39901 tcaatcaccg ggaaacccgc ttcaatgttt aatgccagcc actgttggct attgttcagt
    39961 tccgcttcac cgcgcagttt atcggtcagc atattggcag tagcttcatc ggttacgatc
    40021 aggaaacgtt ctgccgggtg ttcaaaccat agcaaagtgg tcgcgccttc tttaactacc
    40081 tgtttttctt tcgaaggcag ttcactaaag atatttgcca gcgcggcgcg cgcctgaaaa
    40141 ccggcaacac caagcagcac acgctcgtcg tctggcgcga tggtcacttt agagaacacc
    40201 gcatattttt tcagttcagc cagctgcggt tcacgcacgc tgcgccgttc aatccatgca
    40261 aagccatcgc cgtcgcggaa cagacgtaaa ttgctccaca ttttaccttt ggcgtcgcaa
    40321 tgggcggcga gcaggtgctg atcatctgtc atctggctga catctgctgt cacctggccc
    40381 tgcaaatatt tttcgctgtc cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc
    40441 atcagcgtca gtggcaaacg ggcagaagcc gtaggctgac ggggaggaaa aggtgtaaaa
    40501 gccataatag agtcctgagt tgcttaacgg cgtattaata actaatggta aaagagctat
    40561 gaggcaatgc aagtgctttc actacgcgat ctgtgccttc tgcgaggcga cttcccaaaa
    40621 aatgaagccc ttaaattgtt gtgaattaat ttcgcgatcg cgattgtgtt tactgatatt
    40681 cccgtaaaag aagtcgaaaa acacgttaca atagccgggt actggatttt cgtgagacag
    40741 gaagaagatg gacattaaca acaaagcccg cattcattgg gcatgccgcc gtggtatgcg
    40801 cgaactcgat atttcaatca tgccgttttt cgaacatgag tatgacagct taagcgatga
    40861 cgaaaaacgc atctttattc gtctgctgga atgtgacgat cccgatctgt ttaactggct
    40921 gatgaatcac ggtaaaccgg ccgatgcaga actggaaatg atggtccgac tcatccagac
    40981 acggaaccgg gaacgtggtc ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt
    41041 ggctttcctt gctgattcat gggctggttg ccgctgttat tttactcatg ccctggccgc
    41101 tcagttacac cccgttatgg atggtgttac tttcgctggt ggtgtttgat tgcgttcgca
    41161 gccagcggcg tattaatgct cgccaggggg aaattcgctt gttgatggac gggcgtttgc
    41221 gttggcaagg gcaggagtgg agcatcgtca aagcgccgtg gatgattaag agcggcatga
    41281 tgctgcgttt acgttctgat agcggtaaac ggcaacattt atggctggca gccgacagca
    41341 tggacgaagc cgaatggcgg gatttacggc ggattttgtt gcaacaagag acgcaaagat
    41401 aaagatgacg gcaaagcata tgcgctaagc cgtcagtgat atcaggcgta atgctctgcc
    41461 atttcgttga ggatttgctc gcaccagctc tgaatacgct cgtcgctaag gtcatactgg
    41521 ttagtttcgt ccagcgccag ccccacgaac agttgcccgt cagcaatcac cggtttcggg
    41581 ctggtaaatt catacccttc cgttggccag tagccgacga acttcacgcc tttggtcgag
    41641 agtttgtcat gcagcatacc gagcgcatcg aggaaccact cgccgtatcc cagttgatcg
    41701 ccaagcccat acagcgcaac aattttacct tcgaggttca ggtcgtcgag ctgatcccag
    41761 acggcttccc agtcttcctg gatttcgcca aaatcccagg tcgggatacc cagaatgagc
    41821 acatcgtact gctccattaa tttcggggag tcgtccttga ggttatgtaa ggtcaccagt
    41881 tctgggccga taatatcgcg gattttttct gccgccattt cggtgtaaca ggtgctggaa
    41941 ccgtaaaaaa gacccatatt cataacgtaa gcttctcgat tctgctgtgg cttttgcggc
    42001 tagtgtatca gaatcgcttt agtcacggca caataaggca taatggatca aatgagtgac
    42061 cataaggggc gcaagtgaaa caggatctgg cacgcatcga gcagtttctt gatgctctgt
    42121 ggctggaaaa aaatctggct gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga
    42181 tggtggagtg gttgcatcac cgcgggttga cgctggcgac ggcgcaaagt gacgatttac
    42241 aggcattact ggcagaacgg ctggagggcg ggtataaagc caccagctca gcgcgtttgc
    42301 tgagtgcagt gcgccgattg ttccagtatc tttatcgcga aaagtttcgt gaagatgatc
    42361 ccagtgcgca tctcgcttca ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag
    42421 cgcaggtcga acgtttatta caggcaccat taattgatca gccactggag ctacgcgata
    42481 aagctatgct tgaagtgttg tatgctaccg ggctgcgtgt ctctgaactg gtcgggctga
    42541 caatgagtga tatcagcctg cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag
    42601 agcgcctggt gccgttaggt gaagaggcgg tttactggct ggaaacctat ctggaacatg
    42661 ggcgtccgtg gctactgaat ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc
    42721 agcagatgac gcgacagacc ttctggcacc gcattaaaca ttatgccgtg ctggcgggta
    42781 tcgacagcga aaagctgtca ccgcatgtgt tgcgtcacgc ttttgccact cacttattaa
    42841 atcatggtgc ggatttacgc gtggtgcaga tgctactggg ccacagcgat ctctccacca
    42901 cgcaaattta tacgcatgtc gctaccgagc gtctgcggca acttcatcaa cagcatcacc
    42961 cgcgggcgtg atgtctgaaa agaacgggaa gatttatgaa gaaaggtttt atgttgttta
    43021 ctttgttagc ggcgttttca ggcttcgctc aggctgatga cgcggcaatt caacaaacat
    43081 tagccaaaat gggcatcaaa agcagcgata ttcagcccgc gcccgtagct ggcatgaaga
    43141 ctgtgctgac taacagcggt gtgttgtaca tcaccgatga tggtaaacat atcattcagg
    43201 ggccaatgta tgacgttagc ggcacggctc cggtcaatgt caccaataag atgctgttaa
    43261 agcagttgaa tgcgcttgaa aaagagatga tcgtttataa agcaccgcag gaaaaacacg
    43321 tcatcaccgt gtttactgat attacctgtg gttactgcca caaactgcat gaacaaatgg
    43381 cagactataa cgcgctgggg atcaccgtgc gttatcttgc tttcccacgc caggggctgg
    43441 acagcgatgc tgagaaagaa atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt
    43501 ttgatgatgt gatggcaggt aaaagcgtcg caccagccag ctgcgacgtg gatattgccg
    43561 accattacgc acttggcgtc cagcttggcg ttagcggtac tccggcagtt gtgctgagca
    43621 atggcacact tgttccgggt taccagccgc cgaaagagat gaaagaattc ctcgacgaac
    43681 accaaaaaat gaccagcggt aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg
    43741 tgaagtcgat gaaacggcag acttgcccgc ggaattgcct cccttgctgc gccgtttata
    43801 cgccagccgg ggagtacgca gtgcgcaaga actggaacgc agtgttaaag gtatgctgcc
    43861 ctggcagcaa ctgagcggcg tcgaaaaggc cgttgagatc ctttacaacg cttttcgcga
    43921 aggaacgcgg attattgtgg tcggtgattt cgacgccgac ggtgccacca gcacggctct
    43981 aagcgtgctg gcgatgcgct cgcttggttg cagcaatatc gactacctgg taccaaaccg
    44041 tttcgaagac ggttacggct taagcccgga agtggtcgat caggcccatg cccgtggcgc
    44101 gcagttaatt gtcacggtgg ataacggtat ttcctcccat gcgggagttg agcacgctcg
    44161 ctcgttgggc atcccggtta ttgttaccga tcaccatttg ccgggcgaca cattacccgc
    44221 agcggaagcg atcattaacc ctaacttgcg cgactgtaat ttcccgtcga aatcactggc
    44281 aggcgtgggt gtggcgtttt atctgatgct ggcgctgcgc acctttttgc gcgatcaggg
    44341 ctggtttgat gagcgcggca tcgcaattcc taacctggca gaactgctgg atctggtcgc
    44401 gctggggaca gtggcggacg tcgtgccgct ggacgctaat aatcgcattc tgacctggca
    44461 ggggatgagt cgcatccgtg ccggaaagtg ccgtccaggg attaaagcgc tgctggaagt
    44521 ggcaaaccgt gatgcacaaa aactcgccgc cagcgattta ggttttgcgc tggggccacg
    44581 tctcaatgct gccgggcgac tggacgatat gtccgtcggt gtggcgctgt tgctgtgcga
    44641 caacatcggc gaagcgcgcg tgctggcaaa tgaactcgat gcgctaaatc agacgcgaaa
    44701 agagatcgaa caaggaatgc agattgaagc cttgaccctg tgcgagaaac tggagcgaag
    44761 tcgcgacacg ctacccggcg ggctggcaat gtatcacccc gaatggcatc agggcgttgt
    44821 cggtattctg gcttcgcgca tcaaagagcg ttttcaccgt ccggttatcg cctttgcgcc
    44881 agcaggtgat ggtacgctga aaggttccgg tcgctccatt caggggctgc atatgcgtga
    44941 tgcgctggag cgattagaca cactctaccc cggtatgatg ctgaagtttg gcggtcatgc
    45001 gatggcggcg ggtttgtcgc tggaagagga taaattcgaa ctctttcaac aacggtttgg
    45061 cgagttggtt accgagtggc tggacccttc gctattgcaa ggcgaagtgg tgtcagacgg
    45121 cccgttaagc ccggccgaaa tgaccatgga agtggcgcag ctgctgcgcg atgctggccc
    45181 gtgggggcag atgttcccgg agccgctgtt tgatggtcat ttccgtctgc tgcaacagcg
    45241 gctggtgggc gaacgtcatt tgaaagtcat ggtcgaaccg gtcggcggcg gtccgctgct
    45301 ggatggtatt gcttttaatg tcgataccgc cctctggccg gataacggcg tgcgcgaagt
    45361 gcaactggct tacaagctcg atatcaacga gtttcgcggc aaccgcagcc tgcaaattat
    45421 catcgacaat atctggccaa tttagcgtca tcttctctat aaaaaagagc gtggattggg
    45481 tacaatcccg ctcttatcac cgcattttga ctagctcaat aaaagaaatc agaccatgtt
    45541 tgaaattaat ccggtaaata atcgcattca ggacctcacg gaacgctccg acgttcttag
    45601 ggggtatctt tgactacgac gccaagaaag agcgtctgga agaagtaaac gccgagctgg
    45661 aacagccgga tgtctggaac gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct
    45721 ccctcgaagc cgttgtcgac accctcgacc aaatgaaaca ggggctggaa gatgtttctg
    45781 gtctgctgga actggctgta gaagctgacg acgaagaaac ctttaacgaa gccgttgctg
    45841 aactcgacgc cctggaagaa aaactggcgc agcttgagtt ccgccgtatg ttctctggcg
    45901 aatatgacag cgccgactgc tacctcgata ttcaggcggg gtctggcggt acggaagcac
    45961 aggactgggc gagcatgctt gagcgtatgt atttgcgctg ggcagaatcg cgtggtttca
    46021 aaactgaaat catcgaagag tcggaaggtg aagtggcggg tattaaatcc gtgacgatca
    46081 aaatctccgg cgattacgct tacggctggc tgcgtacaga aactggcgtt caccgcctgg
    46141 tgcgtaagag cccgtttgac tccggcggtc gtcgccacac gtcgttcagc tccgcgtttg
    46201 tttatccgga agttgatgat gatattgata tcgaaatcaa cccggcggat ctgcgcattg
    46261 acgtttatcg cgcgtccggc gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc
    46321 gtattaccca catcccgacc gggatcgtga cccagtgcca gaacgaccgt tcccagcaca
    46381 agaacaaaga ccaggccatg aagcagatga aagcgaagct ttatgaactg gagatgcaga
    46441 agaaaaatgc cgagaaacag gcgatggaag ataacaaatc cgacatcggc tggggcagcc
    46501 agattcgttc ttatgtcctt gatgactccc gcattaaaga tctgcgcacc ggggttgaaa
    46561 cccgcaatac gcaggccgtg ctggacggca gcctggatca atttatcgaa gcaagtttga
    46621 aagcagggtt atgaggaacc aacatgtctg aacaacacgc acagggcgct gacgcggtag
    46681 tcgatcttaa caatgaactg aaaacgcgtc gtgagaagct ggcgaacctg cgtgagcagg
    46741 gaattgcctt cccgaacgat ttccgtcgcg atcatacctc tgaccaattg cacgcagaat
    46801 tcgacggtaa agagaacgaa gaacttgaag cgctgaacat cgaagtcgcc gttgctggcc
    46861 gcatgatgac ccgccgtatt atgggtaaag cctcttttgt taccctgcag gacgttggcg
    46921 gtcgtattca gctgtacgtt gcccgtgacg atcttccgga aggcgtttac aacgagcagt
    46981 tcaaaaaatg ggacctcggc gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa
    47041 ccggcgaact gtctatccac tgtaccgagt tgcgtctgct gaccaaagca ctgcgtccgc
    47101 tgccggataa attccacggc ttgcaggatc aggaagcgcg ctatcgtcag cgttatctcg
    47161 atctcatctc caacgatgaa tcccgcaaca cctttaaagt gcgctcgcag atcctctctg
    47221 gtattcgcca gttcatggtg aaccgcggct ttatggaagt tgaaacgccg atgatgcagg
    47281 tgatccctgg cggtgccgct gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg
    47341 acatgtacct gcgtatcgcg ccggaactgt acctcaagcg tctggtggtt ggtggcttcg
    47401 agcgtgtatt cgaaatcaac cgtaacttcc gtaacgaagg tatttccgta cgtcataacc
    47461 cagagttcac catgatggaa ctctatatgg cttacgcaga ttacaaagat ctgatcgagc
    47521 tgaccgaatc gctgttccgt actctggcac aggatattct cggtaagacg gaagtgacct
    47581 acggcgacgt gacgctggat ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga
    47641 ttaagaaata tcgcccggaa accgacatgg cggatctgga caacttcgac tctgcaaaag
    47701 cgattgttga atctatcggt atccacgttg agaagagctg gggtctgggc cgtatcgtta
    47761 ccgagatctt cgaagaagtg gcagaagcac atctgattca gccgaccttc attactgaat
    47821 atccggcaga agtttctccg ctggcgcgtc gtaacgacgt taacccggaa atcacagacc
    47881 gctttgagtt cttcattggt ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgatg
    47941 cggaagatca ggcgcaacgc ttcctggatc aggttgccgc gaaagatgca ggtgacgacg
    48001 aagcgatgtt ctacgacgaa gattacgtca ccgcactgga acatggctta ccgccgacag
    48061 caggtctggg gattggtatc gaccgtatgg taatgctgtt caccaacagc cataccatcc
    48121 gcgacgttat tctgttcccg gcgatgcgtc cggtgaaata agcattacgt tatgcgcata
    48181 accccggcag atgtcggggt tttttaattg tgctgcgcga aagcagacaa caattttctt
    48241 gcttcactat tggctgcctg catcaccatc cacggactga acgcccacgg cgtggcatca
    48301 ataccgtgta atacatctgc taaatcacac cattgataat ccatcacttc atcatcgttg
    48361 atctgtaacg cactggtggt gcgtgcggca aataccggac acacttcatt ttccacaatg
    48421 ccattcggat cggtggcgcg atagcgaaag tcaggataga tagattcagg aggcgtaatt
    48481 tccacgccaa gctcataacg gcaacggcgg atcaccgcgt cttcgttgct ttctcccagt
    48541 tgtgggtgcc cacaaaccga gttagtccac acgccaggcc atgctttttt gctcagtgcg
    48601 cggcgggtaa ctaataattg tcctttggca ttaaacagcc aactggagaa cgcgagatgt
    48661 aagcgggtgt ctgccgtgtg tgcggcatac ttttccagcg tacccgtggg aactccctgt
    48721 gcattcaata aaatgacgtg ttccgtttgc ataatttctc acatgtaatt ctgatcactc
    48781 atcattatag ataattgaag agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc
    48841 ccgttttgag cggatgccga caggtaaaaa ccatttaaaa aagtagcatc atatatttga
    48901 agcgactttc aatggccatt gcaacacagt cagaaatgac aggaatagag agagtaaaaa
    48961 gcaacagcag actgaatata gcgcgcatta aaaaattcat attattctcc atgctcattt
    49021 ttcttcagct tgcgcatcag aagccacata cgtactgtcc tgacttttaa atctttatca
    49081 gagacggatt ccttcaccag gtcagcatga tgctgatagc cattaaagaa gacgttcagc
    49141 acaacggcgc taagggttgc gagcataatg ccgctatgta gcagcggttg cagtacggcc
    49201 ggtaacttag aaaagaaatc gtgagagagc gttggagtca tgccaacgcc gagactgatc
    49261 gccacaatat agaggttgta acggttggtg gtgtagttac agcgcgacag aattcgaatc
    49321 cctgtcgcca gtaccatgcc gaacatcacc agaccagcgc cgcccagcac aaattgcgga
    49381 atggaggcta ccagcaccgc catttttggc accatgccaa acaggattaa tataattccc
    49441 gaagaaatac acacccagcg gctatgaacg cgcgtcacgc taaccaggcc aacgttttga
    49501 gaaaatgacg tgtgggggaa gctgttaaac gtgccaccaa tcattgtccc tacgccatcg
    49561 acacgcagcc cgcgaataat atcgtgcgaa gagagtttac gaccgactat ttcacccagt
    49621 gccaggaaca tccccatcga ctcgataaac acgatgatta acacggcagt catggtcaga
    49681 atggaaacgg gatcgaaaat cggcatacca aacgacatcg gggtgacaat cgcaaaccac
    49741 gaggcatcat gtagcccgga taaattgact tcattcatca tccacgaaag taaaaagcca
    49801 aatacaatcc ccagtaatac ggcgacgttg gacataaatc ctttcgcata gcgagtaatg
    49861 agcaagataa aaattaagac ggcaaaggag atacctaaat aaacgggatt accatattgc
    49921 ggattccctt tacctccggc ggcccagtca atacccacct gaatgatgct aagcccgata
    49981 gaagtaataa ccacaccggt aaccagtggc gggaataaag gcatcaagcg accgataagt
    50041 tgcgctaata atgtggtgat aaaacccgcg gcgatagtgg caccaaatat acccagcagg
    50101 ccgatatccg ggttcatccc aatggctatc attggtgtta cagcagcaaa ggtcaccgac
    50161 ataatcaccg gcaggcggat ccccataaag cggccgatac cgatacattg caataatgtg
    50221 acgatcccgc agcaaaagag atccgagcta atgagcatcg caatagcttc ttttgagagg
    50281 cccagtcggt caccaatcat aagaggaaca gcgactgcac ctgcgtacat gacaaggacg
    50341 tgttgcagac cgagtatgat cagctttcct ggtgataata tgcggtcaac ctcatcagtt
    50401 gggcggtctt gcccagaaga tgagggaagt tgggaatcta tggcgctcat cagtgtttct
    50461 cctttcgccc gccagagtaa aaagcaaccg tttacgttaa tgaaactgtc taaacacatg
    50521 ctcgtggagt gccagttttt gcgaggaagg ggagttagag acaggaattg cggaggcaat
    50581 cacataatcg tgcttaaact acgaaaatga caagagaacc tgacaatgga attatctgta
    50641 attttctcat attgatattt cattaatcat tctggcatgt cgcactctcg catttaatcg
    50701 tttttatctg gatagcgcta tttggggcgg caatgttgaa ataatgaaag cggcatagat
    50761 tgaggggcaa taatttgaac tcagtcgcaa aattataatt aaaacggtca tgctacctaa
    50821 tatatgcccg tttattgcat cgcgaaattt gagaagacat aactcattaa atgaaaggga
    50881 tgcaaatgaa taagtttatc gctgctgaag ccgcggaatg tataggctgt catgcttgtg
    50941 aaattgcctg tgcggtggca cacaatcaag aaaactggcc gctgagtcac agtgactttc
    51001 gaccacgtat ccacgttgta gggaaaggcc tggctgcgaa tccggtggcc tgccatcact
    51061 gcaacaatgc cccttgcgtt acggcttgtc cggttaatgc tctgactttc cagtccgata
    51121 gcgtacaact ggacgagcaa aaatgtattg gttgtaaaag atgcgcaatc gcttgcccct
    51181 ttggcgtcgt tgagatggtc gatacgattg cgcagaaatg cgacctttgt aaccagcgca
    51241 gttccggcac gcaagcctgt attgaagttt gcccaacaca ggcgttacga ctgatggacg
    51301 ataaagggtt acagcagata aaggtggccc gccagcgcaa aacggcagca ggaaaagcgt
    51361 catcagacgc tcagccatct cgcagtgcag cgttgctccc cgttaactcg cgtaaaggcg
    51421 cagataaaat ttcagcaagt gaacggaaaa accactttgg cgaaatctat tgcgggctgg
    51481 atccacaaca agcgacttat gagagtgacc gctgtgttta ttgtgccgaa aaagctaact
    51541 gcaactggca ttgcccgctg cataacgcta ttccggatta catccgtctg gtacaggagg
    51601 gaaagattat tgaagcggca gaactttgcc accagaccag ttccttacca gaaatctgcg
    51661 gcagggtatg tccacaggac cgtctttgtg aaggcgcatg tactttgaaa gatcactctg
    51721 gcgcagtctc tatcggtaat ctggaacgct acatcaccga taccgcgctg gcgatgggct
    51781 ggcgtcccga tgtcagcaaa gttgttcccc gtatcgagaa agtggcggtg attggcgctg
    51841 ggcctgcagg gttagggtgt gctgatattc tggcgcgcgc aggagttcag gtcgatgtct
    51901 ttgatcgcca tccagaaatt ggcggtatgc tgacttttgg cattcctcct ttcaaactcg
    51961 ataaaacggt attaagccag cggcgagaga tattcaccgc aatgggaatc gactttcatc
    52021 ttaactgtga aattggccgc gatattacct ttagcgattt aacttctgaa tatgatgcag
    52081 ttttcatcgg cgtggggact tacgggatga tgcgagcaga tctgccgcat gaagatgcgc
    52141 ccggtgtcat tcaggctcta ccgttcctga ctgcccatac ccgccagctc atgggattgc
    52201 cggagtctga agagtatccg ctgacggacg tggaaggtaa gcgagtcgtg gtattgggcg
    52261 gtggcgatac gacaatggat tgtttgcgga cttccattcg cctcaatgct gccagcgtga
    52321 cctgcgcgta tcgtcgtgat gaagtcagta tgccgggctc gcgcaaagag gtggtcaatg
    52381 cgcgcgagga aggtgtcgag tttcaattca atgttcagcc gcaatatatc gcttgtgacg
    52441 aagatgggcg tttaactgcg gtgggcctga ttcgtaccgc catgggtgag ccggggccgg
    52501 atggtcgccg tcgtcctcgt ccggttgcgg gttcagagtt tgaattgccc gccgatgttc
    52561 tcattatggc ctttggtttc caggcgcatg ccatgccgtg gttgcagggc agcggaatta
    52621 aactcgataa atggggcctg attcaaaccg gtgacgtcgg gtatttacct acccagacgc
    52681 atctgaaaaa agtctttgct ggtggtgatg cagttcatgg cgcggatctg gttgtcactg
    52741 caatggccgc aggaaggcag gcggcgcgcg atatgttaac tctgtttgat acgaaggcat
    52801 cgtgatgaaa tcgttaatta tcgttaatcc ggctgactgt attggctgcc gtacttgtga
    52861 agtggcctgt gtggtcgctc acccttcata acaggagttg aatgccgata tctttttacc
    52921 ccggctgaag gtacagcgac tggactgcat cagtgcgccg gtgatgtgcc atcagtgtga
    52981 aaacgcccct tgtgttggcg cttgccccgt gggggcgctg acgatgggcg agcaggtggt
    53041 gcagaccaat tctgcccgtt gtattggctg tcagagttgc gtcagtgcgt gcccgtttgg
    53101 aatgatcacc attcagtcgt tgccggggga tacccggcaa caaatcgtga aatgcgatct
    53161 ctgtgaacag cgggaagagg ggcctgcctg cgttgaatct tgcccaacac aggcgctgca
    53221 gttgcttact gaaagagaac tcaggcgagt ccgccagcag cgtattgttg ccagcggtga
    53281 gaatcctctg tgaaagaaat ctccgcgctg aaaggcgcgg agaatctatt agattgccca
    53341 accacccgcg taaaatgcga ccagtgcggc agtaatgatc accgtaccaa tattcagctt
    53401 ttgccattcg cgtgcaaaga cgcggcctac gaccagtgtc acaaagccca gcataatacc
    53461 ggtaacgata ttacaagtca gaacgatgaa cacggcacac accaggccag ccatagcgtc
    53521 aataaaatca ttgaaatcca gcttcgagac gttacttaac atcagcaaac ctacgtacat
    53581 cagagcgggt gcagtggcgt aaccagggat caaaaatgac agcggtgata agaacagaat
    53641 cagcaggaat aacgccccca ctacggttgc ggttaaccct gttttacccc cggcggcggt
    53701 tcctgccgct gattcgatat aaaccgctgc gggcgctgcg cccaccaggc cggagaatat
    53761 tgaacttact gagtcactgg tcagggcttt gccgccgttg atgatctggt tgtctttatc
    53821 caacaaattc gcctgaccgg cgacggcacg gatggtgcca gtagcgtcga acactgcggt
    53881 catcaccaat gccagtacac tcggaagtac agttggctgg agtgcgccca taatatcgag
    53941 gctgaaaatc agagacttac catcttcgcc agtcaggctt ggcatcgcca ccagaccgtg
    54001 gtatttcacc gctgggtcaa agattaagcc gatgatcgaa attgcaataa tcaccaacaa
    54061 gataccgccg ggtacgcgac acttctccag accgaagatg accgccagcc ccagcaagct
    54121 catcatcacc gggaaggagg taaacgcacc gagcgccact ggcaagcctt caatcgggtt
    54181 tttgataacc atacccacac cgttagcagc aatcagcagc agaaacagcc cgataccgat
    54241 acctgtaccg tgagcgatac ccatcggcaa attacgtaag atccaggtac gcacaccggt
    54301 tacggaaatg gcggtgaaga tgacgcccat cagaaatacc gcgcccagtg cgacaggaac
    54361 gctaatttgt tgcccgagta ccagactgaa tgcggtaaac gccgtcaagg aaatcgcgca
    54421 accaatcgcc attggcaaat tagcccataa ccccatcagc aacgagccga agcccgcgac
    54481 cagacaggtg gcaacaaaca cagctgcggg aggaaaacct gctttgccca gcattcccgg
    54541 aacgacgata acggaataaa ccatggccag aaaggtcgtt aagccagcca gtacttcctg
    54601 acgaacggta ctgccacgag cggtaatttt aaaataatta tccagcgcgc cctgtggctt
    54661 tggtgcgtcc ggtgtttgta ggatgtctcc agacataatg atgtcctcac gaggatgtaa
    54721 aagttttatt attagttgcg ttcgtacacc agacgaccat caacgtaggt gcggtagatc
    54781 gaacggtcat cgcccaacgt catcatcacg aacaatttgt cgactaaaga aacagagttg
    54841 tcatagcgca gctgctgtag cggagtggcg gtgggttcca tcaccacgaa atcagcctct
    54901 ttgccaggta aaaagttgcc aatcaaatcg tcaaggccca gagatttcgc tccgccgagc
    54961 gtggccaggt aaaacgcttc atatgccgag aggcgatagc cttgtaattg caacactttg
    55021 taggcttcgt tcagcgtttg cagcatgttg aaagtggttc cggcaccgat atccgttccc
    55081 atgcccactt taactttctt ctgccatgct tttttcaagt tgaataaccc gctgccgagg
    55141 taaaggttgg aggtcggaca gaaagcaatg ctggatttgg tttcgctgag acgatcccac
    55201 tctttttctt tgagatggac gcagtgagca aagacacagt ttttaccggt caggccgtac
    55261 tgatggtaaa catccagata accatcatgg tcaggataaa gcgatttcac ccaggcaatt
    55321 tcatctttgt tttcacagag atgggtatgt acccacgtat ccggatattc ctctttcagg
    55381 cgttgcgcca tcgccatctg ttcaggagaa gaagtagggg cgaagcgtgg cgtaatcgca
    55441 tatagcagac gaccattttt gtgccagcgt tcaatcagtt ctttgctttg gtgatagctg
    55501 ctttcggcag tgtctagcag ataatccggt gcgttgcggt ccatcatcac cttaccggca
    55561 atcatacgca tattgatatg actggcggct tcaaacagcg catcaacgga ttgcggatga
    55621 acagtgccaa acaccagcgc cgtggtggtt ccgttacgta aaagctgctt gatgaagaac
    55681 gccgacattt cgcgggcgta ctctaaatcc tcataacgac gttcagtagg gaaggtgtgt
    55741 ttattcaacc actccagcaa ttgttcccca taggccccca ccatttcact ttgcggataa
    55801 tggatatgtg tatcgacaaa gcccggtact atcagtttgc cgcgatagtc gcgcacgcga
    55861 atagtgtcag gaatttgatg ctttccgtct tcccattcgc caaaccattc cacttttccc
    55921 tgtttaatga gtaataaacc atcctcaata aaccgcagcg cagaggcaat ctcttccggg
    55981 ttatcgaccg tacgggtgac atcaataaaa ctgcctcgta ccgcttttaa cgtgtgttct
    56041 cctgacatca ttgactcctt tactgttaat ccatctcttc cgctgaggta ataccaggca
    56101 gaacgttttc ctgtcggtag ccaccaggga ggataatatt taataaaatc gcagttaacc
    56161 cgccagcaca aatagggttt tcaactaata cataaataga ggctggcaat attttaaaaa
    56221 tttcgggatc gtaggagacg ccaagtccta aacctaaaga agtggcgaca ataagtgttt
    56281 ctcggcgctt taaaccgttg gtgatgatga tgcgaatccc agcgatggca atcatggaaa
    56341 acatcaacgt cattgcgcct cccagaactg ccgagggaat ggtcgtgaag aagccgccaa
    56401 tcatcggaaa caaaccgagg ataaccagca ttaccgcgat ggttcgcccg acataacgtg
    56461 aagcgacgcc agtcatctga ataaccccat tattttgcgc aaacgtggtt aagggtaatg
    56521 acccgacagc ggaggcgata acagaaacca gaccgtctgc cagcacgccg cctttcagtc
    56581 gggactgata ctcttctccc tgaatgggtc ggcgggaaac cattgccgtg gcggtgatat
    56641 cgccgacagc ttccagcacg ctaagcagat aaatcgtgcc aaccaccagg aactgatgga
    56701 agctaaaact aaagccgtat ttgaacggat gtgggatggt gattaacggc aaattgcgca
    56761 tactgctgaa atccaccatg cccaggcata acgacgcgat ataaccgacg catagcccaa
    56821 tggcgatccc tcccatgcgt agcaacggac tgcgacagca gttaaagccg atcaccacaa
    56881 tcaaaaccaa taaaccaacg ccgagatgtt cgtaattgcc gaaggtaccg ctgcttttgg
    56941 cagcaaatcc tccaccaaaa tcgataatgc cgactttaat caggcttaag ccgatcatca
    57001 gtaccacaat accgctgaca gtaggcgtaa taacccgacg taaatagggc aagataaatg
    57061 aagatccgac aaccagtaat gcgccaacga aggagacgcc gaggagcgac gacatgatta
    57121 actcttcgtg aaaaccgtcg cttttcatgc tgctgcccag cgcaatcatg accgtaacaa
    57181 atgaaaaatt gactgactga attgagagta ggccagaacc gacgatgcca tagcggttta
    57241 cttgtaacca ggtaccaata ccagaggcga tcatcgccat tgaaacaaga taggcagttg
    57301 tttcagcgga aagctgtaag gccgccccca cgattaacgc gggggttacc atcggaacga
    57361 aaattgccaa cagatgggta atggcaccaa cgagagcctg atgaaaggga gggcgatcct
    57421 ccagttcaaa tataaggtca gaacctgcat ggtttatatc agacatccct tcactccttt
    57481 taaagtaaat ttgatcatct gaattagtaa aataaattcc cacgaatttg gggcgcagga
    57541 cgccttgaga acggcttatc tcattttcgc agtcactatt tagtcatcgt ccaaacagag
    57601 aaacaggccg ccgaagcggc catttattat ttatatcttt tccagcgcgg tgagtatttt
    57661 ctccggtgtg aaatgccatt cgcgtaacca gatgccgcat gcatcgtgaa ttgcggtagc
    57721 aatcgccgga gctgcgccat ttacaccgat ttccgagatc gatttcgccc cgaacgggcc
    57781 gactttatcg tcgctcggca ccagcactgc gcggaaatca cgcggaatgt caccaatttt
    57841 cggtgcgccg tagctgcgta aatcacgcgt taacgggtga ccttcggcgt cataaatgat
    57901 ctcttcgctc atactgtggc cgatagctcg cagggtagca ccgtagattt gtcccaacgc
    57961 taactctgga ttgaccggtg taccgcagtc cagcaaggcg tagaatttat ccaggcggat
    58021 ttcacccgta cgcgtgttga cggcaacttc agcgaagttt gcgccatacg ggaaggcgaa
    58081 atcaggcgtg atataactgc cagttcccac cagtgaacca aagccggtgc cggtttcgcc
    58141 tttatgggca atatccccga aactaacttc gcctttcttg ccgcgcacga cgcccggcgt
    58201 tgctagttga acatctgcca ctggctcacc caacatttgc gcgccgtgga acagaatttt
    58261 ctcccgcaga ttttccgctg ccaaacgcgc cgcgttaccc gagaagcaag taccggacga
    58321 ggcatatgcg cctttatcaa acaacgcatg atcggtatca ccggagataa catgcacgtc
    58381 ctgcggtggg cagtgcagca cttctgctgc cagtttcgtc accacggtat ccagaccagt
    58441 accaatatcc gcaccgccag aatgaacgat aaaggtaccg tctgattcca gtttgatcat
    58501 gcagttagcc tgatcgatat ccgggatccc tgatttctgc atgataatgg cgacaccacg
    58561 accgatgtgc cagtcaccat tttgtggttt tggtgaagac cattggatca tctcgcgccc
    58621 ctgacgcagg atctcttcca gtgcgcagct ggcggcggaa ggaacggagg tcggcgcttt
    58681 accttcaccg attgcaccga gaattttcag ctcttgccct tcgtgtaccc ggttacgttc
    58741 gataatttcc agttggtcga tctgtaactg ttcagccagt tccgctaatg ccatggtgat
    58801 agcgaagtta cctttcggtg cgccataacc ctgataagca ccatttgggc aaatgttgct
    58861 gtagtaggtg gtgacctgga aatcgacgtt atcgcacgga tataacggca gcgacagcgc
    58921 cggtccgtta cacggtacgg tgagtgagtg gttgccgtaa gggccagtgt tggcgcggaa
    58981 atccatcttc actgccgtca ggcgaccatc ttttttcgct cccagtttga cggtgacttt
    59041 cgcgacgtga cgagaggtgt tagcaataaa ctcttcttca cgggtgtagc ggaacagtac
    59101 cggacgcccg gtcacgcagg ttgcccaggc gcacacttct tccagcagga tgtcctgttt
    59161 ggaaccaaaa ccgccgccaa ctcgctcttt aatgacatga actttatgct gtttcatgtc
    59221 cacgaggcgc gcgacctggc ggcgtaagtg ccatggtacc tgggtggagg cgtggataac
    59281 cagacgatcg ccgtccatcc gggtaaagca gatatgtgtt tcagtcgggc actgctgcgc
    59341 ctgcgttgag ttataggttc gctcaatgat cacatcggca tcggcaaagc ctttgtccat
    59401 atcgccaata tgaccatgaa tactagcggc gatatttttg cgagggcgag aaccgatcgg
    59461 gaagttgatg atcatatgct cgccgcgctg ggctgcatgg ctgttatcgt cttccagagt
    59521 atctggcgca ccagcaacat acaccaccgg ttcatcgtgc acgacaggcg catcttccgc
    59581 cattgcttcg tcgatcgaca ttaccggctt aagcacttca tattcaacct cgatgagttt
    59641 caatgcttcg agcgcaattt cttcactttc agcgaccacc gcagcaacgc gatcgccgac
    59701 gtgacgcatt tttttgccga acatacggcg gtcaagcggt gacggttccg gtgcgctctg
    59761 accccccggg gtatagtaga tatcagggca attcaggtga gtaataacgt gaacgacgcc
    59821 cggtaaggct tcagctttgc tgacatccag atgagtaatc agtgcgtgag cgtgtgggct
    59881 acgtaacatt ttaatgacgc aggcgtcagc cgttacgcgg tcttcaacat agcagggttt
    59941 cgcctgcacc attttcgcgg catcagtttt aggataatgc ttgccaatga cttcaaggtc
    60001 gtcacggaaa gtcggagcga tatcaatggt ggcctgcgga ttatttttac gtgcaaccgc
    60061 cagttcaatg acctgatagt attgctgcca gccagcatcg cggctgaaca aaccagaaag
    60121 cgcgtcgtcg atctcttcgc gggtaggtgc ggcgatgcga tcgagaagat cggtgataat
    60181 cagagctgca gctggatcgt tataaccaga ctgcaccacg ccaacatcaa ccattgcctg
    60241 ttgaaccaga cttaactcgt tccatttgcc cagagattct gcggtacgaa tatctgcctt
    60301 ctctaactgt gcggcaataa gcaaggacgc gttaacgata ttaccgttaa agatgattgc
    60361 gtcagaaccg gcaaacccga aaccatcatc actgttgcgt acagagtgca ttcccatgtt
    60421 aaacaacagc ttttgcacgt tttcgcctgg attaacggtt agctcctgag gcgcgccatt
    60481 taaagtaaag tggatgatca tacggcttcc tccccagcct gttggcagtc ggcatacagg
    60541 tcggctacca gaactcccgt gatatagcgt ttataggcca cgctgccccg caaatcttcc
    60601 tgcgggaaaa tggcgttggc gacagcctgt tcaagtgcat tgccttccag attttgtttt
    60661 tcgacatcat gcagacgcag tgctttactg gcgacgccat ccagcgcaat tcgcataccg
    60721 tcgtggtctg tcattgcaac ggctgccgtc acgacggtta aacctgcctg agaacggcta
    60781 attttgcggg tcgcacaggt gcgatacgga tctttaatga taatttcggt tagcaggcga
    60841 tcgcatgggc aggccaggta gtcctcgatt gacagcgttt cgccgttgcc aaaaaccagt
    60901 tcagcatcca gtgccagcag gacgggaagc agcactgact cttcctgccg ggcggcgatt
    60961 tcaccaccaa tggtcgactg attacgaaca tggcgtgagt aaacaaaacc gagggcttca
    61021 cgcagtgctg caggaataaa tcgcgcatca cgcagtggct gcaagcgaga cattgcgcca
    61081 atccgcagtg caccgttatc ccagtcaacc cagtccagtt ccagatcctg taaggaaatg
    61141 gcaatctttt tatcggtacg ggttggtgta gcattgagtt tgctgccccc ggcgaaccag
    61201 acggcttcat cctggtagcg gcgcttcagt tccagcgcct gttcgacaga gtcgggcctg
    61261 aaaaattgtt caatcatctt ctttccttat ttaaccctct tcctgcggga agagggcatc
    61321 tgtcatttat gccagcgcat ccatccgacg ccacatactg gcagcggctt ttctggcttg
    61381 tgcataaatg gaatcgcaat cgaagttaaa ctgacggtct tcatagacca tcacaccatt
    61441 gaccatcacg ctgtgaacgc tgcctgagcc cataccgaaa gcgatatgcc cggcgatatt
    61501 gtctgccagc agcggcgtcg gcgagttgta atcgcaaatg gttaaatcag ctttgtaacc
    61561 ggcttccaga agcccaaatt tcgcgccaaa gttgcggctc atcagttcgt taccgttagt
    61621 cagggctttg gcaaaactgt caggccacag cggaccaccc gcatcgcgat gtttaaagaa
    61681 ggcaaatttc atctcttcaa acatgtccga accaatgccg tccgttccca acgccagatt
    61741 gcggatgtcg ctaaggtgat ggttgtagcc gacatggttg ttcatgtttg aacgggcgtt
    61801 atgcaccagg aacgcatcgc gctgattgag tagggcgatg tcatctttcg acaagtacag
    61861 cccatgagcg accagcgttt tgctatcgat gagatcgaat tgcgccagtc gtgccagcag
    61921 gtctttgccg taccagtggt gactgtagga aacgtcgtaa aggtcttccg cagcgtgaat
    61981 atgcaaacca cggcctgtgg cgttcacggc ttcacgtagc atctccagac cggcatctgg
    62041 cacggtaaac ggcgcgtgag caccgatatg tgcttccacc agatacggct cggttgctgc
    62101 tttcttcgcc tcatcaatct gacgggcgaa gcggatgttt tcttctacac cttcctgcaa
    62161 ctctttgatg ccgttgttac ggtcagtagt ttcaaaacag gtcatcgcgc gcaggccaac
    62221 ttttaaaaat gcgtcgcgca atgtggagag cgacccgccg atatacgccg gagaggcatg
    62281 gtgatcgata accgatgtac atccgctctt aatcgcttcc agggaacaaa tcagcccgct
    62341 gtaatagagc gactcttcat caagggcgcg atcgagccgc caccagagat ttttcagcgt
    62401 tgagatgaaa tctgggctgg gggcgatgtt tgccataatt ccgcgggaaa gccccgagta
    62461 aaaatggttg tgcgagcaga caattcccgg catcacaatc cgaccatgca tctctttgta
    62521 gctggcatcg gggtagcgtt gcgtcagggc atcgccgata gcgacaatca catcattttc
    62581 gatagcgata tcaacgcctt cctgcacttt cgccgggtgt aactgcactg cggtgacatt
    62641 cttcagaatc aacatgatta cacctccacg cggcccagca gataatgatg atgctggtgg
    62701 acatggctga tgatgcggca catatcgttc agctccggtg gtacgttgtt gaactgacct
    62761 tcgctgtcga tgtttaacac ccagctttgg ttattcagtc gtactcgtac ccggcaatct
    62821 tccacaagga agcctgggtt gctgctgtta tcaaagtctt gcgccaggct gaaaacggtg
    62881 attttgtctt tgtatggttt accgttccac ggacagaact gagcgcagtt gccgcattcg
    62941 ttacagtaag cgtcgaggtg cagcgtctgg aatctattct ggaagcctgg gaccgcaatg
    63001 gagacgttgg cgcggttcgg gcagacatcc acacacttgc tgcaaacgta gttacattca
    63061 aggcagcgag cagcttcctg cgcgacaaac gcatcacgat cgtcactgtt caccagcgtg
    63121 atagagatat cgcctttacg ttgatagatt tccgccggat tgacgttgtt ccagtattta
    63181 tcgttctggt gggaacggat attttcccga ctaaggatgg catcggtcgc ccgacgcgcg
    63241 gttccgacag cagcgacaat ggaggatggt ccgcgctgta cgtcgccgat cataaagacg
    63301 tcagtcagac gagtttcgcc attatggtcg acgtctggcc agccgttttt gtccagcggc
    63361 acgcccatcg cattcagggc ttcagtatcc tgctgttcac caatggcggt gatcaggctg
    63421 tctacatgca gtgttactgt ttcgttggtt tcaaccggac gacgacgacc tttctcatcg
    63481 ggttcgccaa gcgacataac gcgcaaggtt aaggtgccat cagcatcgaa acgttccgga
    63541 ttattcagga aacggaactc cacgccgtcg tgcaacgctt cttcatactc ttcgcgccat
    63601 gcgggcatct cttgcagtga acgacggtaa acgaccgttg ctttttctac gcctggaacg
    63661 cgtaacgccg cacgagcgca gtccattgcg gtgttacccg ccccgacaac gaccacatgt
    63721 ttgcccagct tgagcgctgt acccttgttg tattcacgca ggaaggggag tgatttcaag
    63781 acattttggt tgtcgcccgc cagtttcaca ccgctatttt tatctgtgcc ggtggcaatc
    63841 agaacatagt ggaagtcctg attttttaac tgctcaacgg ttaaatcggg tgagcagcca
    63901 tactcaaatt tcacgccgtg agcggcaaca aaatcgatat cgtgctgaat taactcagcc
    63961 ggaattcgga actgaggaat gatatttttc accacgccgc ccgcattggc ttcgcgttca
    64021 aacagcgtaa ccggatggcc cgctctggca aggaagtaac ctgctgccag acccgccgga
    64081 cctgcaccaa tcacggcaac cggatggcgt gaaccagaac cggctggttt gtgccagcgt
    64141 tgcttatatt catcccaacc tttttccagc gcgacttttt tcagttcgcg gatattcagc
    64201 gcactgtcgt aatccaggcg ggtacagttg tattggcact ggtgatcgca aatatgaccg
    64261 gtaatggcgg gcagagcgtt gcgttggtag atgagttcca gcgcgtcggc atagcggtgt
    64321 tcgccaagca gacggatgta ttccggaata tcttgcttga tagcgcaggc agtaacacag
    64381 ggggcaacgt agcagtcggt cagcggcagg tcttctgcca cttcaatacg ctcttctggc
    64441 ttccagtgtt tctgggtgta ttccatggtt aacgcatctg ctgccagtct gttcagtcgt
    64501 tcgacgtcaa catggtcaag tccccaggcg tcggagcctt ccagctcgcg catgcaggca
    64561 cttaagcgca aatagccgcc aggtttcagc aggtcggttg ccatagtaat agggcgaata
    64621 cctgtatcaa aaatatcgcg gatagtcagc tgactggcac caccggaata agaaatgggc
    64681 agtttgccgt caaaggcgcg agagagaact gctgcaacat tgatggagag cgggaacagc
    64741 gcacggcctg acatatacat ctcttcacca ggcagtgcgc ctttattgtt gatggtgccg
    64801 agagtgttag tcagttttac gccaaagccg agtgattttt cttttgccag tgccatcagg
    64861 cgttccagca tttccagtgc ttgcgtcagc ttgaggtcgt gatcaaatga ctcttctttt
    64921 aagcctatgt aaccgaaacc gcagacatcg aggatctcac gaacacgcgc gtaccccagt
    64981 aaggtcgggt taagtttcac aaaggtgttg agcccttttt cttccagcat gtagcggcaa
    65041 atggcttcga tttcatgcgg aggacagccg tgcatggtgg agagggtgac gccatgcacc
    65101 atactggtgg ggatgcgagc gggtaaggct tgcaagcttt cgcgtttttc ctgcaatccg
    65161 tggcgggcta aaaatgcgtc atcctggagt aatttattca gcgtatcgcg atattgcgcg
    65221 aatttcggat ggtcagatgc gtccatcata ttgtcgatga actgttgcat tggcggttgc
    65281 ttaatacctt cgaggttgta accgacgctc atattaaaga tgaacgattt accggaatca
    65341 gaaggttgga acatcgcttc gagaaggtgc agggcaaacc aggctttgag gtattcatcc
    65401 caggctttaa gcagggtaaa ttcggtagac cattcggtgt taaagcactc gtcttcggca
    65461 tcaatacagg gcttttccag ctccaggcgg tcaagaattt ggacggtttt tagttcgatg
    65521 aaacgtccgc cagtcagcca ggaagtgaca atattttgcg cgagctgcgt gtgcgggcca
    65581 gcggcagggc cgacgggagt ggcacaggtt tcaccgaaga cgctaacagt tttacctttt
    65641 acaggtgagt aaaactgttg ctcgggaata ccaaagattg agcgttgttg ttggtattca
    65701 tcaaatatgc gcgtcaaaag ttcctcaaac ggaatgggac gcataatatc ccccataacc
    65761 ctctccttag cttatacaga tatcattgag tgaccggtgt tgagaaatcc ggtggtttag
    65821 ggggatagag caacaatcgc accactcttg ccaggtttgg cgggaaatat aaaaaaatgt
    65881 gaggcggctc gaaacttaag ggctttttgt gaatgaactc acaggtgcta aatgagaatt
    65941 aaaatc
//
LOCUS       DAZKFZ010000006        59964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000006 DAZKFZ010000000
VERSION     DAZKFZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            118..1815
                     /gene="ypdA"
                     /locus_tag="ACHOQW_000353"
     CDS             118..1815
                     /gene="ypdA"
                     /locus_tag="ACHOQW_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP9571493.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1830..2564
                     /gene="ypdB"
                     /locus_tag="ACHOQW_000354"
     CDS             1830..2564
                     /gene="ypdB"
                     /locus_tag="ACHOQW_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP9571494.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2577..3434
                     /gene="ypdC"
                     /locus_tag="ACHOQW_000355"
     CDS             2577..3434
                     /gene="ypdC"
                     /locus_tag="ACHOQW_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9571495.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3437..5932)
                     /gene="ptsP"
                     /locus_tag="ACHOQW_000356"
     CDS             complement(3437..5932)
                     /gene="ptsP"
                     /locus_tag="ACHOQW_000356"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9571496.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCCIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5957..6994)
                     /gene="ypdE"
                     /locus_tag="ACHOQW_000357"
     CDS             complement(5957..6994)
                     /gene="ypdE"
                     /locus_tag="ACHOQW_000357"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9571497.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6994..8079)
                     /gene="ypdF"
                     /locus_tag="ACHOQW_000358"
     CDS             complement(6994..8079)
                     /gene="ypdF"
                     /locus_tag="ACHOQW_000358"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9571498.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8094..9341)
                     /gene="fryC"
                     /locus_tag="ACHOQW_000359"
     CDS             complement(8094..9341)
                     /gene="fryC"
                     /locus_tag="ACHOQW_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9571499.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9363..9689)
                     /gene="fryB"
                     /locus_tag="ACHOQW_000360"
     CDS             complement(9363..9689)
                     /gene="fryB"
                     /locus_tag="ACHOQW_000360"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9571500.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9908..10873)
                     /gene="glk"
                     /locus_tag="ACHOQW_000361"
     CDS             complement(9908..10873)
                     /gene="glk"
                     /locus_tag="ACHOQW_000361"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP9571501.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11077..12333
                     /gene="yfeO"
                     /locus_tag="ACHOQW_000362"
     CDS             11077..12333
                     /gene="yfeO"
                     /locus_tag="ACHOQW_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP9571502.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12448..12774
                     /gene="ypeC"
                     /locus_tag="ACHOQW_000363"
     CDS             12448..12774
                     /gene="ypeC"
                     /locus_tag="ACHOQW_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP9571503.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12914..14152)
                     /gene="mntH"
                     /locus_tag="ACHOQW_000364"
     CDS             complement(12914..14152)
                     /gene="mntH"
                     /locus_tag="ACHOQW_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9571504.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLSKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14488..15690
                     /gene="nupC"
                     /locus_tag="ACHOQW_000365"
     CDS             14488..15690
                     /gene="nupC"
                     /locus_tag="ACHOQW_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP9571505.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15740..17929)
                     /gene="pdeA"
                     /locus_tag="ACHOQW_000366"
     CDS             complement(15740..17929)
                     /gene="pdeA"
                     /locus_tag="ACHOQW_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9571506.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18137..18212)
                     /locus_tag="ACHOQW_000367"
     tRNA            complement(18137..18212)
                     /locus_tag="ACHOQW_000367"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18177..18179),aa:Ala,seq:ggc)
     gene            complement(18252..18327)
                     /locus_tag="ACHOQW_000368"
     tRNA            complement(18252..18327)
                     /locus_tag="ACHOQW_000368"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18292..18294),aa:Ala,seq:ggc)
     gene            18548..18907
                     /gene="yfeC"
                     /locus_tag="ACHOQW_000369"
     CDS             18548..18907
                     /gene="yfeC"
                     /locus_tag="ACHOQW_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9571507.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18909..19301
                     /gene="yfeD"
                     /locus_tag="ACHOQW_000370"
     CDS             18909..19301
                     /gene="yfeD"
                     /locus_tag="ACHOQW_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9571508.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19341..20549)
                     /locus_tag="ACHOQW_000371"
     CDS             complement(19341..20549)
                     /locus_tag="ACHOQW_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9571509.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20557..20982
                     /gene="tnpA"
                     /locus_tag="ACHOQW_000372"
                     /pseudo
     CDS             20557..20982
                     /gene="tnpA"
                     /locus_tag="ACHOQW_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21100..22515)
                     /gene="gltX"
                     /locus_tag="ACHOQW_000373"
     CDS             complement(21100..22515)
                     /gene="gltX"
                     /locus_tag="ACHOQW_000373"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9571510.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22774..22849
                     /locus_tag="ACHOQW_000374"
     tRNA            22774..22849
                     /locus_tag="ACHOQW_000374"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22807..22809,aa:Val,seq:tac)
     gene            22894..22969
                     /locus_tag="ACHOQW_000375"
     tRNA            22894..22969
                     /locus_tag="ACHOQW_000375"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22927..22929,aa:Val,seq:tac)
     gene            23016..23091
                     /locus_tag="ACHOQW_000376"
     tRNA            23016..23091
                     /locus_tag="ACHOQW_000376"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23049..23051,aa:Val,seq:tac)
     gene            23096..23171
                     /locus_tag="ACHOQW_000377"
     tRNA            23096..23171
                     /locus_tag="ACHOQW_000377"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23129..23131,aa:Lys,seq:ttt)
     gene            23291..23632
                     /locus_tag="ACHOQW_000378"
     CDS             23291..23632
                     /locus_tag="ACHOQW_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFP9571511.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
     gene            complement(23623..24549)
                     /gene="yfeR"
                     /locus_tag="ACHOQW_000379"
     CDS             complement(23623..24549)
                     /gene="yfeR"
                     /locus_tag="ACHOQW_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9571512.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            24639..25637
                     /gene="yfeH"
                     /locus_tag="ACHOQW_000380"
     CDS             24639..25637
                     /gene="yfeH"
                     /locus_tag="ACHOQW_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP9571513.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(25634..25852)
                     /gene="ypeB"
                     /locus_tag="ACHOQW_000381"
     CDS             complement(25634..25852)
                     /gene="ypeB"
                     /locus_tag="ACHOQW_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP9571514.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25854..27869)
                     /gene="ligA"
                     /locus_tag="ACHOQW_000382"
     CDS             complement(25854..27869)
                     /gene="ligA"
                     /locus_tag="ACHOQW_000382"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9571515.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(27940..28938)
                     /gene="zipA"
                     /locus_tag="ACHOQW_000383"
     CDS             complement(27940..28938)
                     /gene="zipA"
                     /locus_tag="ACHOQW_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP9571516.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            29168..29929
                     /gene="cysZ"
                     /locus_tag="ACHOQW_000384"
     CDS             29168..29929
                     /gene="cysZ"
                     /locus_tag="ACHOQW_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP9571517.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            30114..31085
                     /gene="cysK"
                     /locus_tag="ACHOQW_000385"
     CDS             30114..31085
                     /gene="cysK"
                     /locus_tag="ACHOQW_000385"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9571518.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            31469..31726
                     /gene="ptsH"
                     /locus_tag="ACHOQW_000386"
     CDS             31469..31726
                     /gene="ptsH"
                     /locus_tag="ACHOQW_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP9571519.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            31771..33498
                     /gene="ptsI"
                     /locus_tag="ACHOQW_000387"
     CDS             31771..33498
                     /gene="ptsI"
                     /locus_tag="ACHOQW_000387"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP9571520.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            33539..34048
                     /gene="crr"
                     /locus_tag="ACHOQW_000388"
     CDS             33539..34048
                     /gene="crr"
                     /locus_tag="ACHOQW_000388"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9571521.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(34091..34942)
                     /gene="pdxK"
                     /locus_tag="ACHOQW_000389"
                     /gene_synonym="thiJ"
     CDS             complement(34091..34942)
                     /gene="pdxK"
                     /locus_tag="ACHOQW_000389"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP9571522.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            35047..35414
                     /gene="yfeK"
                     /locus_tag="ACHOQW_000390"
                     /pseudo
     CDS             35047..35414
                     /gene="yfeK"
                     /locus_tag="ACHOQW_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            complement(35417..36328)
                     /gene="cysM"
                     /locus_tag="ACHOQW_000391"
     CDS             complement(35417..36328)
                     /gene="cysM"
                     /locus_tag="ACHOQW_000391"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP9571523.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(36462..37559)
                     /gene="cysA"
                     /locus_tag="ACHOQW_000392"
     CDS             complement(36462..37559)
                     /gene="cysA"
                     /locus_tag="ACHOQW_000392"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP9571524.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            complement(37549..38424)
                     /gene="cysW"
                     /locus_tag="ACHOQW_000393"
     CDS             complement(37549..38424)
                     /gene="cysW"
                     /locus_tag="ACHOQW_000393"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP9571525.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(38424..39257)
                     /gene="cysT"
                     /locus_tag="ACHOQW_000394"
     CDS             complement(38424..39257)
                     /gene="cysT"
                     /locus_tag="ACHOQW_000394"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP9571526.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(39257..40273)
                     /gene="cysP"
                     /locus_tag="ACHOQW_000395"
     CDS             complement(39257..40273)
                     /gene="cysP"
                     /locus_tag="ACHOQW_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP9571527.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(40431..41222)
                     /gene="ucpA"
                     /locus_tag="ACHOQW_000396"
     CDS             complement(40431..41222)
                     /gene="ucpA"
                     /locus_tag="ACHOQW_000396"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP9571528.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(41351..42208)
                     /gene="murR"
                     /locus_tag="ACHOQW_000397"
     CDS             complement(41351..42208)
                     /gene="murR"
                     /locus_tag="ACHOQW_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP9571529.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            42372..43268
                     /gene="murQ"
                     /locus_tag="ACHOQW_000398"
     CDS             42372..43268
                     /gene="murQ"
                     /locus_tag="ACHOQW_000398"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9571530.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            43272..44696
                     /gene="murP"
                     /locus_tag="ACHOQW_000399"
     CDS             43272..44696
                     /gene="murP"
                     /locus_tag="ACHOQW_000399"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP9571531.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            44701..46005
                     /gene="pbp4b"
                     /locus_tag="ACHOQW_000400"
     CDS             44701..46005
                     /gene="pbp4b"
                     /locus_tag="ACHOQW_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP9571532.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(46063..46962)
                     /gene="yfeX"
                     /locus_tag="ACHOQW_000401"
     CDS             complement(46063..46962)
                     /gene="yfeX"
                     /locus_tag="ACHOQW_000401"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP9571533.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(47058..47633)
                     /gene="yfeY"
                     /locus_tag="ACHOQW_000402"
     CDS             complement(47058..47633)
                     /gene="yfeY"
                     /locus_tag="ACHOQW_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP9571534.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(47694..48143)
                     /gene="yfeZ"
                     /locus_tag="ACHOQW_000403"
     CDS             complement(47694..48143)
                     /gene="yfeZ"
                     /locus_tag="ACHOQW_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP9571535.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(48130..48555)
                     /gene="ypeA"
                     /locus_tag="ACHOQW_000404"
     CDS             complement(48130..48555)
                     /gene="ypeA"
                     /locus_tag="ACHOQW_000404"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP9571536.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            48769..49638
                     /gene="amiA"
                     /locus_tag="ACHOQW_000405"
     CDS             48769..49638
                     /gene="amiA"
                     /locus_tag="ACHOQW_000405"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP9571537.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            49642..50541
                     /gene="hemF"
                     /locus_tag="ACHOQW_000406"
     CDS             49642..50541
                     /gene="hemF"
                     /locus_tag="ACHOQW_000406"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP9571538.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(50547..51599)
                     /gene="eutR"
                     /locus_tag="ACHOQW_000407"
     CDS             complement(50547..51599)
                     /gene="eutR"
                     /locus_tag="ACHOQW_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP9571539.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(51645..52145)
                     /gene="eutK"
                     /locus_tag="ACHOQW_000408"
     CDS             complement(51645..52145)
                     /gene="eutK"
                     /locus_tag="ACHOQW_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP9571540.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(52158..52817)
                     /gene="eutL"
                     /locus_tag="ACHOQW_000409"
     CDS             complement(52158..52817)
                     /gene="eutL"
                     /locus_tag="ACHOQW_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP9571541.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(52827..53714)
                     /gene="eutC"
                     /locus_tag="ACHOQW_000410"
     CDS             complement(52827..53714)
                     /gene="eutC"
                     /locus_tag="ACHOQW_000410"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP9571542.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(53735..55096)
                     /gene="eutB"
                     /locus_tag="ACHOQW_000411"
     CDS             complement(53735..55096)
                     /gene="eutB"
                     /locus_tag="ACHOQW_000411"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP9571543.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(55108..56511)
                     /gene="eutA"
                     /locus_tag="ACHOQW_000412"
     CDS             complement(55108..56511)
                     /gene="eutA"
                     /locus_tag="ACHOQW_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP9571544.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(56508..57734)
                     /gene="eutH"
                     /locus_tag="ACHOQW_000413"
     CDS             complement(56508..57734)
                     /gene="eutH"
                     /locus_tag="ACHOQW_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP9571545.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(57834..59021)
                     /gene="eutG"
                     /locus_tag="ACHOQW_000414"
     CDS             complement(57834..59021)
                     /gene="eutG"
                     /locus_tag="ACHOQW_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP9571546.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            complement(59011..59847)
                     /gene="eutJ"
                     /locus_tag="ACHOQW_000415"
     CDS             complement(59011..59847)
                     /gene="eutJ"
                     /locus_tag="ACHOQW_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP9571547.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(59858..>59964)
                     /locus_tag="ACHOQW_000416"
     CDS             complement(59858..>59964)
                     /locus_tag="ACHOQW_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP9571548.1"
                     /translation="TTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
BASE COUNT        14254 a        16425 c        15153 g        14132 t
ORIGIN      
        1 acaccattta tctatcaatc cccttttaaa attgtggttt ttcctcaaaa acggcctgct
       61 ggtcatcagc cttcaggtta cctatcatag aggtttaatc cttattcaga gtcacccgtg
      121 cacgaaatat tcaacatgct gctggcggtc ttcgatcggg cagcgttaat gcttatctgc
      181 ctgttctttc tcatccgtat ccgcctgttt cgcgaactgt tgcacaagtc ggcacactca
      241 ccgaaagagt tgctcgccgt taccgccatt ttctcgctgt tcgccctctt cagcacctgg
      301 tccggcgttc ccgtagaagg ctcgctggtg aacgtacgta ttatcgcggt gatgtccggc
      361 gggattctgt ttggcccgtg ggtgggcatc attaccggcg tgattgcggg tattcatcgt
      421 tatttaattg atatcggtgg cgtgacggcg atcccctgct ttatcaccag cattctggct
      481 ggttgtatat cgggctggat caacctgaaa atccccaaag cacagcgctg gcgcgtcggt
      541 attctcggcg gcatgttgtg tgaaacgttg accatgattc tggtgattgt ctgggcacca
      601 actaccgcgt tggggatcga tatcgtctct aaaatcggga ttccaatgat cctcggcagc
      661 gtctgtatcg gctttattgt gcttctagtg caaagcgttg agggcgaaaa agaggccagc
      721 gccgcgcggc aggccaagtt ggcgctggat atcgccaaca aaacgctacc tctgtttcgc
      781 catgtcaata gcgagtcatt acgcaaagtt tgtgaaatta ttcgcgatga cattcacgcc
      841 gatgcggtgg cgattaccaa taccgatcat gtgctggcct atgttggtgt gggtgaacat
      901 aactatcaga atggcgatga cttcattagc ccgactaccc gtcaggcgat gaattacgga
      961 aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac cagagattca ttccatgctg
     1021 gtgatcccat tgtgggagaa aggcgttgtg accggaacgc tgaaaattta ctactgccac
     1081 gcgcatcaga taacctcgtc attacaggaa atggcggttg gtctgtcgca aatcatctcc
     1141 acgcaactgg aggtttcacg cgccgagcag ctacgtgaaa tggcaaataa ggcagagctt
     1201 cgcgccctgc aaagcaaaat taatccccat ttcctgttta acgctttgaa cgctatttca
     1261 tcgtcgatcc gtctgaatcc ggataccgct cgccagctga tctttaatct gtcgcgttat
     1321 ctgcgctata acattgaatt aaaagacgat gagcaaatcg atatcaaaaa agagctgtat
     1381 caaattaaag actatattgc cattgagcag gcccgctttg gcgacaagct gacggttatc
     1441 tatgatattg atgaagaggt gaattgctgc attcccagcc tgctgatcca gccgttggtg
     1501 gagaatgcca ttgtccacgg tatccagcct tgcaaaggta aaggcgttgt caccatcagc
     1561 gttgcagagt gcggcaatcg ggtacgcatt gcggtgcggg ataccgggca cggcatcgat
     1621 ccaaaggtga ttgagcgggt cgaagcgaat gaaatgccgg gcaataaaat tggcctgctg
     1681 aatgtccatc atcgcgtgaa gttattgtat ggcgaggggc tgcatatccg ccgcctggaa
     1741 ccaggaacgg aaattgcgtt ttacattcct aaccaacgca ccccagtcgc ctcacaggct
     1801 acgttattgc tttgagccgg agtgatattg tgaaagtcat cattgttgaa gacgaattcc
     1861 tggcacaaca ggaactgagc tggctaatta aagagcacag ccagatggag attgtcggca
     1921 cctttgacga cggtctggac gtgttgaagt ttttgcagca taaccgcgtc gacgccattt
     1981 ttctggatat caatattccg tcgctggatg gtgtgttgct ggcacaaaac atcagccagt
     2041 tcgcccataa accgtttatt gtgttcatca ccgcgtggaa agaacatgcg gtagaagcgt
     2101 ttgaactgga ggcgtttgac tacattctaa aaccgtatca ggagtcacgc attaccggga
     2161 tgctgcaaaa actggaagca gcctggcaac aacagcagac cagcagcacg cctgccgcga
     2221 cggtaacgcg tgagaatgac accattaatc tggtgaaaga tgagcgaata atcgtcacgc
     2281 caattaacga tatctattac gccgaagcgc acgagaaaat gacctttgtt tatacgcggc
     2341 gtgaatccta cgtaatgccg atgaacatta ccgaattttg cagcaaactg ccgccgtcgc
     2401 attttttccg ctgccatcgc tcattttgtg tcaatctgaa caaaatccgc gaaatcgaac
     2461 cgtggtttaa taacacctat attctgcggc tgaaagatct ggattttgaa gtgccggtca
     2521 gccgcagcaa agttaaagaa ttccgccagt taatgcatct ttaatcggga actttcatga
     2581 aagcaccagg cttgcccgcc gatcaacaat ttttcgccga tctgttcagc ggcctggtgc
     2641 ttaacccgca actactgggg cgcgtctggt ttgccagcca gcctgcctcg ttgccggtgg
     2701 gcagtttatg tattgatttt ccccgtctgg atatcgtgct gcgcggcgaa tacggcaatc
     2761 tgctggaagc aaagcagcaa cgtttggtgg aaggagaaat gctgtttatt ccggcgcgcg
     2821 cggctaattt accggtcaac aacaaaccgg tgatgctgtt aagcctggtg ttcgctccga
     2881 cctggcttgg gttatcgttt tacgatagcc gcaccacgtc attgttgcat cctgctcgcc
     2941 agatccagct tcccagcctg caacgcggtg aaggtgaagc gatgcttacc gccctcaccc
     3001 atcttagccg ttcgccgctg gagcaaaata tcattcagcc gctggtgtta agtttgctgc
     3061 atctttgccg taacgtggtg aatatgccgc cgggcaattc gcagccgcgc ggcgattttc
     3121 tctatcacag catttgtaac tgggttcagg ataattatgc ccagccgctc acccgcgaga
     3181 gcgtggcgca gttttttaat atcacgccca atcatctgtc aaaactgttt gctcagcatg
     3241 gaacgatgcg ttttatcgag tatgtgcgtt gggtgcgaat ggcgaaggcg aggatgattt
     3301 tgcagaaata tcatttgtca attcatgaag tggcacagcg ttgcggtttt ccggatagcg
     3361 actatttttg tcgcgttttc cggcgtcagt ttggtctgac gccgggagag tacagcgccc
     3421 gttttcaggg ctaacgtcag aaggttaatt ctgtttccag cagcgtcagg atactttgtg
     3481 catcttgcgc ggcaaacaac gactggcgga agtttttatt caccagttta cgcgccagct
     3541 gcgagaagac tttcacatga ttcatccctt cgttagcacc cagcgtcagc atgatcacca
     3601 gttcgacttc gcccatttct gactgccagt caaccggttt cgccagccgg gcaatgctga
     3661 tactggaatg acggatccac tgagatttgg tgtgcgggat cgccacgcca aaaccaacgc
     3721 cagtggtgac aatctcttcc cgctgccaga catcctcttc cagctcgaac ggatgttcag
     3781 tgcgcccgtt aacgccgagg ttaccgcaca ggaactggat cgcctgctct ttattgctaa
     3841 aggcctgatc aacgaagata ttctccagcg ccagcagcgg gcgaacgtct tcttccggcg
     3901 taaaggcggt gagtaacgct tcaatttcct gcgcgctgcg acattcacac gcctgacgtg
     3961 ccagttcccg acacgcctcg ctatccagtt gacgaagctg gcttttcacc gccggaatgc
     4021 gcgggctact catactcagc tcatccaggc ccagcccaag cagtagcggc agataacggc
     4081 tttcaccgcc cagttcaccg caaatgccta cccatttgcc ccgctgatgc gcagtggtaa
     4141 ctatttgctg caacatgcgc aggaacgatg gcgtaatcgg gttatatagc ggcgatacgc
     4201 gcgggttatt acgatcgacc gcatacagat actgggtcat atcgttggag ccgatactga
     4261 agaaatcgac ctcatcgcag aagtgatcga tgatgcagca caccgacggc acttccacca
     4321 tgatacccag cgtaatcgtc tctgcatgac gcaggccatc gcgcttaagc tcaacgatcg
     4381 ctttttggat ctcgcccttc acccataaga tctgatcgag gccgtggacc atcgggatca
     4441 tcaactgggc gttgccgaaa ctggcggcgc gcaaaatggc ccgcagttga gtgcggaaca
     4501 ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc gaggaacggg ttctcttcct
     4561 ggggaatatt cagataagga atgcttttat cgcctccgat gtccattgtg cggaagataa
     4621 tcggcttgtc gcccgctgcc agtagcacct gctggtaggc ttcaaattgc tcctgctcgt
     4681 ccggctcgct gtcgcgatcc atatagagca tttcggtacg gaacagcccg acgccttccg
     4741 cgccgttggc aaacgcgcca ggcgcttcca gagcggtgcc aatattcgcc gcgatatcaa
     4801 tacgcttgtt atcacgggaa taagccagct gcgcggcagc ctgcgcctgt tgtttttggc
     4861 gtttatccgc cagcgtctgc gcgacctgat aataaccgct caccgcgtca ttcgggttaa
     4921 tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg cccggcataa cgcgcgatcg
     4981 catccagcgg caggccgctc agtaccggga tcgccgaggc gcgggccaga atcagggtat
     5041 gcgaggtgcg cccggttttc tccagaatca tgcccgcaag attttttaaa tccaggctta
     5101 aaaactggct tggggttaaa tcctcggcca ccagaatagt cggtttttcc agcaccagat
     5161 tgttgcgcgg cttcagttcc ggccaggtaa tatgcagcaa ctgttcgcta atatcgcgaa
     5221 tgtcgctaac acgttcgcgc agataatcgc tggcagaagc agaaagtttg gcgcaaacct
     5281 gctccatatt tctgatgatc gccgccccca gcccctgatg ctgttctgtc atcaggcgac
     5341 ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa atgggcgctg aggatagttt
     5401 tgctttcgcc gtcacgctca cgcaattgct ggttcagttg ctcggcaagc gttgccaggc
     5461 tgtgctccag ccgggtggaa tcttgcgcac tggcggggat tgcccgataa ctgtcgaggc
     5521 tgtcgctttg taacagggtc agcgtaccca cgccgacgcc gcttgccagc acattgccgt
     5581 acagtaaatc cgggttaagg cggcttaatg aacgcggcag cggatgcgcc gtcagttctg
     5641 cctgcgtagg ctgaacgctg tcgctgtcga taaagcgcac ctggatgtac tcttccagca
     5701 cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag gctgcaactg tcattaaata
     5761 gggtgccggt gccaatcagc gccagcgagc ttttggcatc ggcctttgcg ttctggcgat
     5821 ggttaataaa agtgatttcg ctttgccact ggctgcactg ttctttaagt tcccacgccg
     5881 gacgagcatg tagaccgtta ggcaaaggac agagaaattg aatcgttaac ataaccgctc
     5941 cgggttaatc aggagatcat ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac
     6001 gttgaataag ggcagataaa agttgctcca tctgcaaaat gtcacggcaa tcggcaatcg
     6061 acgcggcgca atgtccgtgg cgggtggcag gccccatcac cactgtgggt acgccagtac
     6121 cggttaagtg caccgctcca ccgtccgtgc cgccgttact gaacatatcc gcctgtaacg
     6181 gcacgccaat ttctgccgcc acggtttcga tccaggcggt gagttttggc ggcgcaatca
     6241 gtgacttgtc gcttaacacc agcatcgggc cgttaccaat ctggcgatgg ttagccgcgc
     6301 cataattaaa gtttttcgcc cagcaggcgg tatcaaggac aatggcgacg tccggcgaca
     6361 ccgcgcgggt ggcagtttgc ccgccgcgta accccacctc ttcgctggaa ctggccacca
     6421 gccacacttc cgcaggcagt tcagcgctgt gtagttcgcg cagtaacgtc accagcaggt
     6481 agcaaccgag gcggtcatca aaggctttcc ccatcacccg ctggtggggg agaacctgaa
     6541 aagtggtatc gaacgtgacg cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat
     6601 acgagcgcgc gccaatatcc acgcgcatgg cgctgacatc attcccctgc cggtcgccgt
     6661 caagcaggcc tggaattttg cactcttcac gggtggtgat gcgcaccggc tgcagctggc
     6721 gggcagccat gcgtacattg ccaaccggca gcacatcaat cgccccttcg cgggagatgc
     6781 tgcgcaccat aaatcccact tcgtccatat gcgcacagat catcaccttc ggacctgtcg
     6841 attcattgag gcggatcagc accgatccca gcccatcaaa tcgcacttct ttttgcaggc
     6901 gatccgcttc ttccagcagg atctgccgca cttcctgttc cgaggaggcg atcgcatctg
     6961 cctcgctcaa cgctttcaat agcgataaat ccattatgcc tctcccgtga gcaacactgt
     7021 tttcggcatg gcgtagagca cttctgcgcc ttgcggggtg accagcacaa catcctcgat
     7081 gcgcacgccc ccttgccctg gcaaataaat ccccggctcc acggtcagta acatgcctgg
     7141 ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggatct tcatgaactt caatgccgat
     7201 agcatgagcg gtgttatgac cgaaatagtg gctaaaacct gcctcggtaa tcacccgacg
     7261 cgcggcttcg tcaacctgct ggcagcgcac gccggggcga attgcagaga ttgctgcgag
     7321 ctgtgcctgc aaaacaatct gatagacgtt aaacagcggg tgagattcgg cgctcacccc
     7381 ttcgccattc accagcaagg tgcgcgtcat atcagagcag tagccctgat agagcgcacc
     7441 gaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg
     7501 cagcgcccca cgccagccac tggcgacaat ggtgtcaaaa gaggtttttt ctgcgccctg
     7561 ctggcgcata aaccactcca gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat
     7621 aaagcggcga atatgctctg caccgcgatc ggcaatccca caggcaaggc ggattttctc
     7681 cacctcctct ggcgttttga tttgccgcag cacatccggc gtggcgctaa ccagtttcgc
     7741 attgagttca gactgccagc gatgcgcggt ttcccaactc acctgctggc cttcaaaacc
     7801 gagcgtttgc aactgctcat cggcaatgat ttgcctggcg atggttgtaa gcgtgtgcgt
     7861 cgcgtcaagc aaatgcagct ggtaaccttg cgtgcgggct tctacatccg cgtaatagcg
     7921 cgagtccacc agaatgtgcg cactttcacg gctaatcagc acatagcctg atccggtgga
     7981 gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat ccagttgttg
     8041 cgccttaagc cagtcgcgca gcgaagcgag taatgtcatc tggattttcc ttcttacagg
     8101 ctatcgatta acaatttgcc tttacggaac atcatcagac gcaggaacac caccatcagc
     8161 gcagttatga ctgcccccag cgcgatcccc gccatataga cgcccaggtt agtgaccagc
     8221 ggccatgccc agatagcgga ttccgggaac cattgcactg cgcccagcca gacagcggcg
     8281 gttgagccga caatcgcgcc gaccatatac gacggaatgg cggtgatggg gctttccagc
     8341 gcaaacggaa tcgcaccctc actgatcccc ataaaggcaa ggaacatcgc ggtcttaccc
     8401 tgcggataaa gttgtgcgtt gaacaggcgt ttgccggtta aacgacggtc aataatggtc
     8461 gccagaccca gaccaatcgg cggaataacg atagcgatag aacgagcggt gaccggtaat
     8521 acgtggtcag tggtaaagct gaaggcaaca aaacctgcgg ctttgttgat cgggccacca
     8581 aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct
     8641 gcggcagtca gcacagtacg gataccgccg ttgatccagc ccccaaaggg ggtgatgacg
     8701 tagtacatcg ccagcatgac aaaaatagcg gaaagaatcg gtagcaaaaa tgtggttttg
     8761 aacgctaaca ggaaatccgg taactggatt ttctggttca tccatttcac aaggtaaccg
     8821 gcaacgatag aaataatcag cgcaccaatg aaggtggacg gcaccggcga agaggtcgcc
     8881 cattgcatag tgctgggatc gaagttcagc agttgcgtcg gctgggtaga catcaagccg
     8941 ccgataaaac cagccggaaa cgccagtttg ccaccgatag agttcgccac aaaagcagca
     9001 aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg actgcgacaa ccaggcaaat
     9061 ttcagtaagg agaggtcgaa accggagaat ttcccgctat taagagcatc catgatgccg
     9121 atctcagcag gaattttaag ccagctataa gcaatcaact ggctgaaagc gaggatcaca
     9181 ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg acatcacatg ctgcgggagt
     9241 tcaccccaga agctgctttt agaggcctgc ggattcttaa ctgccgctgc cgcaccggat
     9301 gcgccaggca caacggttgc actgcgtttt ttaatggcca taacaattat ccctgtcgaa
     9361 tattactgtt gttcagaggc aatcatctct tcgatttctt tgatgatgcc cgcagcgttt
     9421 ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg ttcgttatct
     9481 tccggggtaa ctgccacgga gtggatgatg atggtcgctt cggcgatatc ctgcgccgtc
     9541 aggcgattct ggataccgtc cgcgccctgg gtttcaattt tcacttcata accggcttct
     9601 accgccgctt cttccagcgc ctgagcggcc ataaaagtgt gagccaggcc cattgggcag
     9661 gcacataagg caatcagttt cttactcatc gttaattcct cgttcagtga tagttgacga
     9721 gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc atttactgga
     9781 gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc ttgtgagggg acacaaagaa
     9841 aagttgaatt aactgtatga tttaaaagat tatcgggaga gttacctccc gatataacag
     9901 gaaggattta cagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac
     9961 ccggattgtc atggacgatg agatacaccg gaatatcatg gacatattct ttaaagcgcc
    10021 ctttatcttc aaatgcggca cggaaaccag aggctttgaa gaactcaagg aagcgcggca
    10081 cgataccgcc cgcaataaac acgccgccaa atgtcccgag attgagcgcc agattgccgc
    10141 caaaacggcc cataatgacg caaaacagcg acaacgcacg gcggcaatcg gtgcagctgt
    10201 cagccagcgc gcgttcggta atatcttttg gcttgagatt ttctggcagg cggttgtcgg
    10261 ctttaacaat tgcgcgatac aaattcacca gtcctgggcc agaaagcacg cgttccgccg
    10321 aaacatgacc aatttccgca cgcaatattt cgaggataat ggcctcttct tcactattcg
    10381 gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac ccaacgctta tcgacatgga
    10441 ccagatgcgc aaccccaagc cccgttccgg caccgtaaac cgcaataggc ttaccttcta
    10501 ccggttctgc gccaccaaac tgaatcagat gctctttttt cagcatcggg atcgccatcg
    10561 atacagcggt aaaatcgtta ataatttcca gatggctaaa accgagattc tttttcattt
    10621 cggcaattga gaacgcccag gtatggttgg tcattgccac ccagtctccg gtaatcgggc
    10681 aagcgatggc aatacagccg tctttcacct cgaccttatg ttcttcaaga taaacgcgaa
    10741 tgaccgcttc gaggctgggg taatcaagcc ctgaataggt cttagcctgc gagatttcac
    10801 cactggcaat atcacacaga gcaagacgtg cgttggtgcc gcccacatca ccgactaatg
    10861 catactttgt cattcttcaa ctgctccgct aaagtcaaaa taattctttc tcacactgta
    10921 aatacctggg ggcataacaa caacgccgga aaggcaggct ccctgtaaat atcgatctgg
    10981 gtcacacaat tactttatcg tttcagcacc aattgcagcg atgccttttt gcaagctggg
    11041 caaactaagt atctgacccc gcataaggaa tagaacatgc tccatccgcg agccagaacc
    11101 atgttgttat tatcgctccc cgccgtggca attgggattg cgtccagtct tattctgatt
    11161 gtggtgatga aaatcgcctc ggtattacag aatttgctct ggcaacgact gccgggaact
    11221 ctggggatag cccaggattc acccctctgg atcatcggtg tattaacgct aacgggtatt
    11281 gcggtggggt tggttatccg tttcagccag ggtcatgccg gaccagaccc cgcctgtgaa
    11341 ccgctgatcg gcgcaccggt tccgccctct gcgctaccag gacttatcgt agcattaatt
    11401 ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc cgatcatgac cgtcaatatc
    11461 gcccttgcgg ttgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact
    11521 attttagcct ctgccggaac catcggcgcg ctgtttggta cacctgttgc ggcggcgttg
    11581 atattttcgc aaaccttaaa tggcagtagt gaagttccgc tatgggatcg tctttttgcg
    11641 ccgttaatgg cggcagcagc tggtgcactt actaccggat tatttttcca tccccatttt
    11701 tcactgccca ttgcgcatta cggacagatg gaaatgaccg atattctcag cggtgcaatt
    11761 gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat ggtgcttacc acggttgcac
    11821 gcgatgatgc atcaaatgaa aaatccggtg ctcgtgctgg gtattggcgg atttattctc
    11881 ggtattctgg gggttattgg tggaccagtt tcgctgttta aagggctgga tgagatgcag
    11941 cagatggtgg caaatcaggc tttcagcacc agcgattact ttttgctggc ggtaattaaa
    12001 cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat cttcccggca
    12061 gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc acgttcccgc cgtaccagcg
    12121 gcaataaccg tttcctgcgc tattctcggc atcgtgctgg tggtaacacg cgatggctgg
    12181 ttaagtcttt ttatggcggc agtcgttgta cccaatacca cattgctacc gctgctctgt
    12241 atcgtcatgc ttccggcatg gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt
    12301 ccgaagcaac agccacccca cgataacgtt tagcaaaaaa tgctctcctt tatgatttaa
    12361 gagttatggc gatgatacgg ggctttacct ccccgtaata ttgcgttaac aggccgctga
    12421 cggcttcgat cgtgaaggag aataacaatg ttcaggtcac tgtttctggc ggccgccctg
    12481 atggcattta ccccgcttgc agcaaacgca ggcgaaatca ccctactgcc atcaatcaaa
    12541 ttacaaattg gcgatcgcga tcattacggt aattactggg acggtggtca ctggcgcgac
    12601 cgtgactact ggcatcgcaa ttatgaatgg cgtaaaaacc gctggtggcg tcatgataat
    12661 ggctaccatc gtggctggga taagcgtaaa gcatatgagc gtggctatcg tgaaggctgg
    12721 cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg gacaccgcca ttaaacacat
    12781 tcgccaatgg agcacaaggc ctgatgcgac gctagtgcgt cttatcaggc ctacaaaccg
    12841 gctctttaac gcgtagggcg gataaggcgt ttacgccgca tcgccaatag tgccagatgc
    12901 gacgctcatt caactacaat cccagcgccg tccccaccaa taaccagata ttcagcgcca
    12961 cgaccagcac tacaatcacc cagcctgtct gttttacgcg tttgctgttc accagatcgc
    13021 ccatcaactt gctgtcactg gtgaaaatca gcagtggaac cagcgccagg gcgataccaa
    13081 aacttaacag cacctgactc ataaccagaa tccgtgtcgg atctaatccc atcagaatga
    13141 caataaatga cggcagcatg gtgactgtac gacgcaccca cagcgggata tggaagcgaa
    13201 tgaacccctg catcaccacc tgccctgcca gtgtccccac caccgttgag gacagtccgg
    13261 cagcaaccag acttaaccca aagaccgttg ccgcagcatg gcttaacagc ggttgcagcg
    13321 tcaaataagc ctcatcaaga tcggcaacac cagtatgacc agaaaagtgg aacgccgccg
    13381 cagctgtagc catcatcgcc agattgacaa aaccggcaat agtcatggcg atagccacat
    13441 cccatttggt ggcggaataa cgttgttgac gcgaaccgcc atgtaaatgc tgagtgagcg
    13501 aagagtgcaa ataaatcaca tgcggcataa tcgtcgcccc taacacgcct gctgccagga
    13561 agaccgcctc cgaagtaggt aaactcggga tcaccattcc tttactcagc tgcgccaggt
    13621 taggctggga gaaaatcaac tcgacaatgt aagccgcggc aacaaacaac agtaacccgc
    13681 caatcacttt ctccagcggt ttttgcccgc gacgttgcag cattaaaatc aggaaagtcg
    13741 cgatccccgt cagcaccgcg ccctgcaaca acgaaacacc aagaatgagt ttaaaaccga
    13801 tcgccgcacc aataaattcc gccaggtcgg ttgccatcgc aataatttct gcctgaaccc
    13861 aatagaacca cactacggga cgcggatagt gatcgcgaat ctgctccgcc agatttttac
    13921 cggtggcaat ccctagtttg gcagagagga tctgaatcag catcgccatc aggttggccc
    13981 aaacgacaac ccacagtagc tgatagccga agctggcacc cgcctgaata ttggtcgcaa
    14041 agttaccggg atcgatataa ccaatcgccg caatgaacgc aggtcccatt aatgcgagcc
    14101 tcatcttgcg cgccgcccgt ccgctgctac tctcaacgcg atagttcgtc atcttgtgcc
    14161 tctaaaacat agcctttgct atgtttcatg ctatgccaaa cgagaatgat tatcaaattc
    14221 atttaaatgg gttgtggtga tttctctgat agaccaggat tatgactacg aaaagattga
    14281 tgacttgaat gttatggtta tgtttaatgt tagcacattt acataacttt cagcttccat
    14341 acacaacata gcagaaatgt atgacagctc actatttttg aagcttgtca caggacgtca
    14401 ttatagtgtg tgtcagatct cgttttcctt aaccatgtta catagaatgt gcacggaaat
    14461 ttaacctgcc tcatatttgg agcaaatatg gaccgcgtcc ttcattttgt actggcactt
    14521 gccgttgttg cgatcctcgc actgctggta agcagcgacc gcaaaaaaat tcgtatccgt
    14581 tatgttattc aactgcttgt tatcgaagtg ttactggcgt ggttcttcct gaactccgac
    14641 gttggtttag gcttcgtgaa aggcttctcc gaaatgttcg aaaaactgct cggatttgcc
    14701 aacgaaggga ctaacttcgt ctttggtagc atgaatgatc aaggcctggc attcttcttc
    14761 ctgaaagtgc tgtgcccaat cgtctttatc tctgcactga tcggtattct ccagcacatt
    14821 cgcgtgttgc cggtgattat ccgcgcaatt ggtttcctgc tctccaaagt caacggcatg
    14881 ggcaaactgg aatcctttaa cgccgtcagc tccctgattc tgggtcagtc tgaaaacttt
    14941 attgcctata aagatatcct tggcaaaatc tcccgtaacc gtatgtacac catggcagca
    15001 acggcgatgt ccaccgtttc tatgtccatc gttggtgcat acatgaccat gctggaacca
    15061 aaatacgtcg ttgcagcgct ggtactgaac atgttcagca cctttatcgt gctgtcgctg
    15121 atcaaccctt accgtgttga tgcctgtgaa gaaaacattc agatgtccaa cctgcacgaa
    15181 ggtcagagct tcttcgaaat gctgggtgaa tacattctgg caggtttcaa agttgccatt
    15241 atcgttgccg caatgctgat tggctttatc gccctgatcg ccgcgctgaa cgcactgttt
    15301 gctaccgtga ctggctggat tggctacagc atctccttcc agggcatcct gggctacatc
    15361 ttctatccga ttgcatgggt gatgggtgtt ccttccagtg aagcactgca agtgggcagt
    15421 atcatggcga ccaaactggt ttccaacgag ttcgttgcga tgatggatct tcagaaaatt
    15481 gcctctacac tctctccgcg tgctgaaggc atcatctctg tgttcctggt ttccttcgct
    15541 aacttctctt caatcgggat tatcgcaggt gcagttaaag gcctgaatga agagcaaggt
    15601 aacgtggttt ctcgcttcgg tctgaagctg gtttacggct ctaccctggt gagtgtgctg
    15661 tctgcgtcaa tcgcagcact ggtgctgtaa gaccatacat aaaaaagccg gggataattc
    15721 cccggctttt ttacgcctgt taatcagcta atggctgcgg gcgaccaatc aaataccctt
    15781 gcagatattg caccccgagc ttatgcaata gcgcctgttg ctgttgcgtc tcgacaaact
    15841 ccgcgaccac actcaatgac ttcgctttcg ccagatcggt aattgatctc acaatcatcg
    15901 catccagcgt attcgtgaca atatctttca caaagacgcc atcaattttg atgatatcag
    15961 cctgcaaacg ctttaaccgt tcgtagttgg catagccggt gccaaagtca tcaatcgcaa
    16021 tccggaaacc aaacttatgc agctgctcga tgttgtacat gctgctttct gcgttagaaa
    16081 acgcctgctc ctcggtgatc tcaagaatga ccgcctgcgg ggagatgtga taacgcttaa
    16141 atagacgaat aatccgcccg gcaatattct tttgcagcag cgtgagcggc attaaattaa
    16201 ctgaaaagcg cgggcctttt ttgtcgcaag ggtgtgttgc cagccacttc aacaaggatt
    16261 ccagcacttg caaatcaaaa cgcgcgctaa ggttaaactg agcaataagg ggcagaaact
    16321 tatccggggt cataatgccg ccgtcatatt tcagtcgcgc gaggacctca tcataacctt
    16381 caccctcttt gttgcgaatt ggctgggcgt agagcagcaa atcaccctga tctaacgccg
    16441 tgcgaatggt attcagcaat agcacctgtt tagtggtctg cccggaaacc atctcctctc
    16501 ggctatccag cgccagcaca tgatgatgtg cgcaggattg ctccgccagc cagcttaact
    16561 gccccaacaa gggttgcagg gtttcctgat ttccatcaaa acgcccccag gcagcaccat
    16621 agcccatatc cagcccggta ttgttccagt gaatttgccg actattcagg atgttaacca
    16681 tatgctggag tcgcccttcc gtttcaggcc cgctcagcac taacagcagt tcactacccg
    16741 gcaattgata caacttttcg ttttcctgca tcaatggcag cagcgtacgg taaattgatc
    16801 ggatacagtg aacacgcatc atcaacccgt aatgacgact cataaactca agattatcaa
    16861 tgcgcaggca gcaaaaactc ttgcccgcct cttgctccgg cgcttgttcc aacgcacgaa
    16921 agttgggcag gagcgttaac ggatcggtca gcgcctgcaa atgccagcgg cgattaagcc
    16981 attcactgcg atgataaatg cgcaccatat agagcaggca aacgctaaag gaaatcagca
    17041 ccgcgagaat aaatgccagc gaatattcgg tttccacccc ttgcaaaaag ttctggttgt
    17101 aattcagcag gcaaagcgtt gaaacagccc aagtgagatt taaaaacgga tagcgaagct
    17161 taccgacccc gagggtaaag atgatgaaga aaacgggtac caggtaaccg gcaataaagt
    17221 cgttttcata aggtgtgcac aacagaagta gcagcacgct taacgctgcc agccagctta
    17281 aggtaaatgc gcgtttctct ttgcccaacg acggagcgat atccctgcgc cacaatatct
    17341 gcgcaaagtg gggacttaca atcatgcgag tgagatagta gaaaagcatg ttgtaaatca
    17401 gcacagcggt gaaaaggctt agcaaatcaa cgaccgtgaa aatggcatcc gcatcgccga
    17461 aaaaggtgga tatctttagc gggaaatcaa agaagctccc cacaagatac atgctgcatt
    17521 tgatgccgat cggcgtcacc aaaccaagcc agaacagacg ttgccagaca tatcggctgg
    17581 tcaatccata acgccaacgc gtccccagct gccagcgtaa tattgcgcag gcacacaaca
    17641 cgacaaacgt ctggctaccg agcatgaccg cagtctgtaa aaaagagaga tgaaagttcc
    17701 acagattggt gcaaaacatc ccgcataaaa cgggaacgac accgcgccag ccaaagataa
    17761 acaagattga cagcattacg cacagtggca tccacgccag aaagatataa ctggaatgga
    17821 taatggcaag tggcgaaata aaacgggata gctgaataag taccacggtg agcgtaaacg
    17881 ctagtgtgaa tatcttgata ttttttatca ggttatgctc cacaaacatg agtacactac
    17941 tctcactgat cggggttttc aggcacacaa ataataagtt tattcgcatt gcgaagcaaa
    18001 caggggccat cctgttgccg atttttcatc caatgatgct ttcaccaggc ggcactaacc
    18061 cggacgtaga ctgtattaca aaagcggcaa aaagcagaga caaaaaaccc ccgctttgca
    18121 gcgagggttg gaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    18181 tgcaagcgct ctcccagctg agctatagcc ccacgatgcg tttacgtacc aaatttgctg
    18241 ggtgcaaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa
    18301 gcgctctccc agctgagcta tagccccgtc acgtaaagct tgtcgagttg acgggcggca
    18361 tcatatgaat tccgcccgaa tgtgtcaacg gcaaattgca acgtgtagtt tcaatcgctg
    18421 aaaaatcagg caaatgaaca attttggaaa accgctcgca ttcagtagtt attcatgtca
    18481 cggtttcctg taaagtggtg ctataaaatg aactactaat agacccacat acattcaggg
    18541 aattgttatg ttcaaggaga ggatgacacc agatgaactt gccagactga ccggctatag
    18601 ccgccagacc attaataaat gggtacgcaa ggaaggctgg acaacgtcac caaaacctgg
    18661 cgtccagggt ggcaaagccc gactggttca cgtcaatgaa caggttcgtg aatatattcg
    18721 taatgctgaa cgtccagaag gtcagggaga agcgcctgcc ctttctggtg atgcgccgct
    18781 tgaagtattg ctggtgacac tggcgaaaga gatgacgcca gttgaacaaa aacagtttac
    18841 atccttgctt ctgcgggaag ggattatcgg attgttacaa cgcttaggga ttcgcgatag
    18901 caaataatat gaaaagatta cgcaataaaa tgaccaccga agaactggct gaatgcctcg
    18961 gtgtggctaa acaaactgtt aaccgttgga tcagagaaaa aggctggaaa acggaaaaat
    19021 ttcccggcgt gaaaggcggt cgtgccagac ttattctggt cgatacgcaa gtttgcgagt
    19081 ttattcagaa cacgccagcc ttccataaca caccgatgct tctggaagcc gaagaacccc
    19141 ttgccgaata cgcaccgggg attcgcactc ctgcttatcg gcaaatcatc agtgcaatag
    19201 acaacatgac tcacagtgag caggaaaaag ttgcgcaatt tttgtcacgc gaaggaattc
    19261 gtaatttcct tgctcgtctc gacatagatg aatcagcttg acatcctcca cgccctgaat
    19321 gacgaggatc cctgctacgt tcaggctgtc gcctgaatca tttcggtggg ttcctgcttc
    19381 aacgagcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc cgccgctaaa
    19441 atgttacgag cgccgtttac atcggcgttc gctgtatatc cacatacctg gcatctgaat
    19501 ttactttgtg acaggcggtt ttctttcgct gtatgaccac agcacgcgca acgctggctt
    19561 gtgtacgctg gcggaacagc cagtacctga ccgccacgcc agagctgctt gtactcaagc
    19621 tggcggcgca tttcatacca gccctgatcc agtatcgaac ggtttaaacc tgattttgcc
    19681 cggacattgc gccccggctg gctgaccgta cccgctgctg actttgacat gttgctgacc
    19741 ttcaaatcct caatgactat cattgcgtgg tttttgctga cggtcgttgt gactttgtga
    19801 aggtagtccc tgcggatatt tgcggtacag gaatgcagtc gctgtatttt gcgtttctgc
    19861 ttctgccagt tattgctgaa tttgaccttg cggcttaact gtcgctgaag tctcgccaac
    19921 ttcttctggt ttttctgaaa actgtttaca ggctcaaaga ctgtgccatc tgacagcgtg
    19981 gcgagtttag ccacgccagc atccagtccg accattgatg ctgaagggtg aaccggagtt
    20041 gatacttcac tttctgtctg aatactgatg taccacttac cgcaggactg gctgacagtg
    20101 acatttttca caacacccgt gacctgacgg ctgttcaggt agcgcatcca gccaagtttc
    20161 ggcagaaaaa tacggctgtt ttcctgatcg agcttaacgc cctgcgggta gcggaatgca
    20221 tcattctgtc cccgcttttt gaatcgggga aaagcagccc gcttccggaa gaagtttttg
    20281 taagcccgct caagctcttt cagtgactgt tgcaatggct gtgacggggc atctttaagc
    20341 cattgcgttt cagtggcgtt tttccactca accagccagg aagccatttt cccgtaaggg
    20401 atgtatttat taccggcctc atgattctca ttctgaagcg ccagagcacg attgaaaacg
    20461 aaacgacatg cgcctgcgaa gcgcctcatt tcacgctcct gttgaccacc tggtcttaac
    20521 tggaatttaa atgcctgtag tcgtttcaca ggccaaatga tactttggtc tatgaataaa
    20581 gaaaccgata tccgacgggg aaggcattgt gttttcctga tgcatgttca tctgttcttt
    20641 gtcaccagat accgacgtca gatttttgac tatgacgcga cagaaaaact acgcacttac
    20701 ttttcaaatg tatgtgctga ttttgaagct gaactggttg aaatggatgg cgaaccagat
    20761 cacgtccatt tgttaactaa ctatcctccc aaactggcga tatccagtct ggtaaacagc
    20821 ctcaaaggcg tatcgggtag gttactgcga cgagatcgac cagatattgc agtcaggtat
    20881 tactacaaag gcgttttgtg gagtcctggc tattttgcca gtagctgcgg aggtgcgcca
    20941 atatccgtca tccgccaata cattgaacaa cagcaaacac ctggtcaggt ggaaaaccgc
    21001 gccttatatc cccgccctga aggacggggt tttacggcgc accggataaa taaaaaacgg
    21061 caggggtatc tctcctgccg tttatctttt atgcgctaat tactgctgat tttcgcgttc
    21121 cgcaataaaa gccagcgctt tgttgatacg ctcgatactg cgggtcttac cgatcgcgtg
    21181 aacggtaacg tccagcgccg gagactgccc cgcaccggtt acggcgacac gcagcggcat
    21241 accaacttta cccataccca cttccagctc atccgccgtc gcctgaatag cgtgatgaac
    21301 gttttcagct gtccagtcag taatcgcggc cagtttgtca cgaaccactt ccagcggctg
    21361 acgcgctacc ggacgcagat gttttttcgc ggcgtcggca tcgaactcag caaaatcttc
    21421 gtagaaataa cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag
    21481 tttcaccaga tcagccagct gcgggccgtt acgggtatcg atattttcct gctcaatgtg
    21541 ccactgtaag tgagtagcaa catactccgg cggcagcgcg ttaatgtagt gatggttcag
    21601 ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa
    21661 gtatttgatc atctcttcac gagtgaagat ttcctgatcg ccgtgggacc agcccagacg
    21721 caccagatag ttcagcagtg cttctggcaa ataaccgtca tcacgatact gcattacgct
    21781 gactgccccg tgacgtttgg acagtttttt gccgtcatcg ccgttgatca tagaaacgtg
    21841 cgcgtaaacc ggtaccggcg ctttcagggc tttaagaatg ttgatctggc gtggcgtgtt
    21901 gttgatatgg tcttcgccac ggataacgtg ggtgatttcc atatcccagt catcgacaac
    21961 cacacagaag ttataggttg gggaaccatc ggtacggcgg ataataagat cgtccagttc
    22021 ctggttgctg aactcgatcg gaccacggat ctgatcgtca aaaacaacag aaccttcctg
    22081 cgggttagca aaacgtacga cacacggctc atcatcagcg tgatgctcat gactgtggcg
    22141 gcagcgaccg tcataacgcg gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc
    22201 caggcgctct ttagagcaat agcatttata agctgtgccc tcttccagca tctgatcgat
    22261 caccgcgttg tagcgatcaa aacgtttggt ctggaagtac ggaccttcat cccactccag
    22321 gctcagccag ttcatgccat ccataatggc ttcgatagct tccggcgtgg aacgctcaag
    22381 atcggtgtct tcaatacgca gcacgaactc accgccgtga ttacgtgcaa aaagccagga
    22441 gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg
    22501 agttttgatt ttcatgaaat ggccttacgt ttagaaagat gccgacaacc ggcaaatcct
    22561 ggaaaaattg agtgggcgat attctatcac tcccgcctga ttcctcaatg tagttcgggt
    22621 tgcaacctta cgcattattg ttttcgttga caaattgcgc aacacggtct gttttgcgat
    22681 catcttgctt aaatttacga cgaacgaaca atttctttag aaaatgcgtt gactcatttt
    22741 gaactctccc tataatgcga ctccacacag cgggggtgat tagctcagct gggagagcac
    22801 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccaa ctactttatg
    22861 tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat tagctcagct gggagagcac
    22921 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac tttctcacca
    22981 gctaaatttc ttgtaaaaat gtgaagtacc gaagtgggtg attagctcag ctgggagagc
    23041 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc
    23101 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
    23161 acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatttgtaa
    23221 gattgatttt tcttctcaag ttccttgttc ttctgccgat attacctcgt acagtgacga
    23281 ggactgaaca atggccacta tctcttcaac ttcaattcct tccatacaaa cacagagtag
    23341 taacagggca tcacaaggta gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat
    23401 catcaaactc actcaacaca ttaaagagat tgtagacact agcggcagtg ctgaagacaa
    23461 gcaaaaacag gccgaattga ttcaacagca aatcacccta ctggaaacac aactggcgca
    23521 gttgcagaga cagcaggcag aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt
    23581 atcccttcta aatcctgttg aaaatacgac tcatatcggt atctatatct gatacaacgg
    23641 atccccttcc cgcccagcca ttagcgtatt gccctggtct cgtaccacgt cccataacgc
    23701 ttcagcggcg gtggaaagtg aacggttttt cctacgcacc agcatcaact ggcgttccac
    23761 caccggcgta atgcgcttta ccaccagcgg gctaccttca ggcaatggta gcgccagtgc
    23821 gggaagaata ctgatgccga tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc
    23881 aatctcctgc acaatattcg cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg
    23941 tcggctgcct gacgcataat cctgcaacac caatttcgct ccctgtaatg cctgccaggg
    24001 tacataatct tccgcggcca aagcgctatc ccgatggcaa agcagaaaga aaggctcgga
    24061 aagaatcact tcacattgca gatcgcccac tgggcctgga tcgatgacga tgccaaagtc
    24121 gacatctccc tggcgaatac tttccatcac ccactgctgc ggacgatcgt gcaggacaaa
    24181 ctgaatatct ggatagcggc gatggctttc agcaatgcat tgcggaataa gatgcgcgga
    24241 aatggtctgg ctggcagcga cccgaacttt tccgctcagt tgttgcccca tacgcccggt
    24301 atcgcgcaac gtgctgttaa gttcatccag cagtcgctca agacgcaaag ccaactgctg
    24361 ccctgcatct gtaagcacca cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg
    24421 attttccagc tccttcacac tgtgacttac tgccgactgg ctcaggccga tacgctctcc
    24481 tgcacgacta aaacttttct cctgcgctac tgtgacgaaa acctttaatt gttttaaaga
    24541 ataattcatc tgttttcttc atgaatggat gcaataaatc aattttattt ctcaaactgg
    24601 aagaagcaca atagaaccat cgatcatctg gagtctttat gaaacttttt cgtatcctcg
    24661 atcctttcac cttaaccctg atcacggtgg tgttgctggc ctctttcttt cctgccagag
    24721 gcgatttcgt ccccttcttt gaaaatctga ccaccgcagc tattgccctg ctgttcttta
    24781 tgcacggcgc gaagttgtcg cgtgaggcga ttattgctgg cggtggtcac tggcgactgc
    24841 atttgtgggt aatgtgcagc accttcgtgc tgtttccgat tctgggtgta ctgtttgcct
    24901 ggtggaaacc ggtaaatgtc gacccgatgc tctactccgg ttttctctac ttgtgcattc
    24961 tcccggctac cgtgcagtct gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg
    25021 cggcggtttg ttctgcgtca gcatccagtc tgctggggat tttcctttca ccattgctgg
    25081 ttggtctggt gatgaatgtt cacggtgcag ggggcagcct tgagcaggtc ggtaaaatta
    25141 tgctgcaact gctgctgccg tttgtgttgg ggcatctttc ccggccgtgg attggtgact
    25201 gggtgtcgcg caataaaaaa tggattgcga aaactgacca gacgtccatt ctgttggtgg
    25261 tttatacagc gttcagcgaa gccgtcgtta atggtatctg gcataaagtt ggctggggat
    25321 cattgctgtt tatcgtggtg gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg
    25381 tctttatggc acgccgactg ggcttcaata aggcagatga aattactatc gtcttttgtg
    25441 gttcgaaaaa gagtctggca aatggcatcc cgatggcaaa cattctgttc cccacatcgg
    25501 tgatcggtat gatggtgctg cccctgatga ttttccatca gatccaattg atggtctgtg
    25561 cggtgctggc gcgtcgatac aaacgccaga ccgaacagtt acaggcgcag caggaaagca
    25621 gcgccgataa agcttaaagc ggacgcttca ggggctggac cagttgcgtc agcccctcgg
    25681 ttttaatcag cagcgtgatt tgcattagct caccgagctt ccctgccggg aactcatctt
    25741 tgcgggcaaa ccacagcaga tactcctccg gcaggtcgat taagcgacgc cctttgtatt
    25801 tgccaaacgg cattatcgta ttggctattt caatcagctg ctctttttcc atctcagcta
    25861 cccagcaaac gcagcatttc cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc
    25921 gccagtttag atcctgcagc ttcacccgct atcaccagat cggttttctt cgacacgctg
    25981 cccgcgactt tcgcgcccag ttcgaccagt cgagctttag cgtcatcacg cgacatctgg
    26041 cttaagctgc ccgtaagcac cacggtttta ccagcaaacg ggctgtcaat ctcttccgcg
    26101 ttgataacga tcggcgcagg ccagtgaaca ccttccgcca acagctcgct gatgacattg
    26161 cggttgcttt cttcggcaaa gaagttgtga acgtgggatg caacgacaat gccaacatca
    26221 ggcacctttt gcagctcttc aatcgaagcg gcttccagcg cttccagcgt tccgaaatat
    26281 gccgccagac ctgctgcggt ggcctcgccg acttcacgga tgccaagtgc atagaggaag
    26341 cgagcaaagg tggtttcttt cgctttttcc agcgcgttaa ccacgttttg tgccgatttt
    26401 ggccccatac gctccagtcc ggtcagtttg cctgcggtga gtttgaacag atccgccgga
    26461 gtgtggacat attctttttc aaccagctga tcgatgattt tgtcgcccat tccgtcaaca
    26521 tccatcgcac ggcgggaaac aaagtgtttc agcgactctt tacgctgcgc accgcaaatc
    26581 agaccaccag tacagcgggc aaccgcttca ccttccacac gctcaacgtc agaaccacat
    26641 accggacaat acgtcgggaa tacaacctca cgggtatctt ccgggcgttc agaaagcacg
    26701 acgttaacca cctgcgggat cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg
    26761 cgtaaaccaa gacgttcgat ttcatccgca ttgtgtaagg ttgcgttact caccagcacg
    26821 cctgcaacat ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc
    26881 tgaaactcga cgtcacgcac aaaggtcatc tgttcctgcg ccgggaattt aaacgctacc
    26941 gcccagcgcg gggctcgcgc gacaaagcca agctgctcct gctgttccag tgagttgacc
    27001 ttaatcacca cgccgtcgat atcaaagccc agcgtcgggc ggtcttcttc cactttgtga
    27061 tagaacgcca gcacttcttc cgccgattca caaagcgtta cccgatcgct gaccggcaac
    27121 ccccactttt taaattgcag taaacggcca agatgagtat ccggcagctc gccaccttcc
    27181 agaacaccaa cgccatagca gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca
    27241 agctgacgca gtgaaccagc tgccgcatta cgtgggttag caaacacttt cccgcccgtg
    27301 cgtcgcgcat cttcgttaat cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc
    27361 acttccagac gcgccgggat attctctccg tgcaacttca gtggaatggc gcgaatagta
    27421 cgcacattag acgtgatatc ttccccggtg gtgccatcgc cacgagtcgc ggcactgact
    27481 aaaacgccat tttcatacag aatactgacg gcaaggccat ccagcttcag ctcacagcac
    27541 caggtgactt tctcgttgct tttcagacgg tcctgcacac gtttgttgaa ggcaagaaag
    27601 ctttcttcat caaaaacgtt atccagcgac agcattggca cttcatggcg aatctggctg
    27661 aaagccgcca gcggcgcagc acctacacgt tgggtaggcg aatcaggcgt aatcagttct
    27721 ggatgtttgg tttccagctc gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga
    27781 atttccggcg catccatcac atgataaaga tattcatgat ggcgaagcgt cgttcgcagt
    27841 tctgtcagtt gttgttcgat tgattccata tcgcaccatc aatgctaaaa acccccgaca
    27901 agcgggggtt cgaagaggag ttaatttgcc ttaagtgtat caggcgttgg cgtctttaac
    27961 ttcgcggatg atgtcctggt actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc
    28021 gtcaagcacg acaccgccca cttcatcggc aatatgctgc gcagattgca gcatcagctt
    28081 gaagttctgc agctcgtcac cgtaagacgg tacctgcata aagatagtga cacccggagt
    28141 agtgaaatcc ttcatttcag gatcaaaggt tcccggtttc accatattcg ccaggctaaa
    28201 taacgccggg ccgctgccat ccgggctaag atgacgatgg taaatattca tatcgccaaa
    28261 aatgaagccc gcttgttgaa tgctgttaag aagcagttca ccgtttagct cgctaccgtg
    28321 atgcgccgcg acgttcatga taatcaccgc ttctttgcgc ttcggtttat ccataactgg
    28381 agccggttcc gctacaggct caggctgtgg tgccgctacg ggttctgcag gctggaaagc
    28441 ctgttgtgcc ggttgcggtg ctgaatgcac tggctgcggc gctggcgcga cctgtggcga
    28501 aactggctgc tgcaacggct gttcaggctg cggctgatag gcaggctgct gcacgggttg
    28561 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
    28621 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
    28681 cggacgagca gcctcatgct cctgggcgtt agccggggca tgattcacgc ggtgaacacg
    28741 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
    28801 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
    28861 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
    28921 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
    28981 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
    29041 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
    29101 ttgcgcaatt acccggtaaa atacgcacaa ttttcctggg attggtcaaa aggagctcat
    29161 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
    29221 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
    29281 caatattttg ttgatggggg gcgcattctg gtggctcttt acgcaactcg atgtctggat
    29341 cccgactctc atgagttatg ttccggactg gttacaatgg ctgagctatc tgttgtggcc
    29401 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
    29461 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
    29521 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
    29581 cgaatggcaa aaatttgcct ggtatctgcc acgcgcaatt gtattgctaa ttctttactt
    29641 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
    29701 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
    29761 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagtct
    29821 gtttacgatg atcccgctgc ttaatctgtt cattatgccc gttgccgttt gtggcgcgac
    29881 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
    29941 ttattttgta aaccgtttgt gtgaaacagg gggggcttat gccgcccctt attccatctt
    30001 gcatgtcatt atttcccttc tgcatataga tatgctaaat ccttacttcc gcatattctc
    30061 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
    30121 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
    30181 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
    30241 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
    30301 ttgaactggt tgaaccaacc agcggtaata ccgggattgc actggcctat gtagctgccg
    30361 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
    30421 tgctgaaagc gttaggtgca aacctggtgc tgaccgaagg tgctaaaggc atgaaaggcg
    30481 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
    30541 aattcagcaa tccggcaaac cctgaaattc acgaaaagac caccggtccg gagatctggg
    30601 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
    30661 ctggcgtcag ccgctacatt aaaggcacca aaggtaagac cgatcttatc tctgtcgccg
    30721 ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg
    30781 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
    30841 tggtcgataa agtcattggc atcaccaatg aagaagcgat ttctaccgcg cgtcgtctga
    30901 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgct gcggcgttga
    30961 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccgtcatcgg
    31021 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
    31081 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt ttcaggtgct tttttgtggc
    31141 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
    31201 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
    31261 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
    31321 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
    31381 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
    31441 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
    31501 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
    31561 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
    31621 gactctgggc ctgactcaag gtacagttgt gactatctcc gcagaaggcg aagacgagca
    31681 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
    31741 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
    31801 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
    31861 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
    31921 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
    31981 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
    32041 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
    32101 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
    32161 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
    32221 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
    32281 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
    32341 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
    32401 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
    32461 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
    32521 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaagtagaa
    32581 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
    32641 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
    32701 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
    32761 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
    32821 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcaa tggatcgtaa agagatcctg
    32881 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
    32941 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
    33001 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
    33061 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
    33121 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
    33181 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    33241 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    33301 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    33361 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    33421 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    33481 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    33541 gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat
    33601 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    33661 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    33721 cgcgcctgta gacggcacca ttggtaaaat ctttgaaacc aaccacgcat tctctatcga
    33781 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    33841 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    33901 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    33961 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtga ctgtgggtga
    34021 aaccccggtt atccgcatca agaagtaatt cttgctgcag tgaaaaatgg cgcccatcgg
    34081 cgccattttt ttatgcttcc gccagcggcg gcaaaatcaa ttcatcgctc tcatgctgct
    34141 gggtgtagcg catcacttcc agtacgcgca accccgcgcg gtgcaccgca tcgtttaatg
    34201 ccttcccttt cagcaaacca ctgataagct gagcacaaaa caggtcgcca gtccctttca
    34261 ggtcggtttt tacccttgaa tgggaaatga cattcacgct gtcggcagtg accaccacaa
    34321 ccagcatctc ctgattttct tcattaccgg aggcgctggt aatcaccacc cattttagtg
    34381 tgtctgaaag cagacttttt gcggcagcaa tggcactgtc gagatcgcgg caatttttac
    34441 cggtcaggat ttccaactca aagatattgg gggtaattcc ctgcgccagc ggcagtaaat
    34501 attgtcgata cgcttcggga aggtcaggtt tgacataaat tccgctgtca atatcgccaa
    34561 tcaccggatc gaccatgatc aataggtcag gatggtcttt gcgtagcgca gtcagccact
    34621 cggcaaggat tttgatttgc gatgccgttc ccatatagcc cgtggttaca gcacgaagtt
    34681 ggcgcagcgc atcacgctcc tgaagtgcac gcaaatagcc gctaaaccat tcatccggaa
    34741 tcgcaccacc gtagaaagtg tcataatgcg gcgtattgct cagcaatacc gtcggcacgg
    34801 caaagacatt caggccgttc tgtttgatag caggcacggc aatgctgttg cccacgctgc
    34861 cgtaaaccac ctgcgactgc acggcgacga tatccgcctg cagcgccctg ctcttatcgt
    34921 taaacaacaa caaactactc atttaatttt ttctccttgc cgatgatcct catcgtattc
    34981 caaccgaaac tttacctgat tctggcagtc aaatcggcta ttacaaaaca aggataaggt
    35041 aattcagtga agaaaatcat ttgtctggtc attacactac taatgacact ccccgcttac
    35101 gcgaagttaa ctgcccatga agaagcccgc atcaacgcca tgctggaggg attagcacag
    35161 aaaaaggatt tgatatttgt gcgcaacggt gatgaacata cctgcgatga agcggtttct
    35221 catctgcgtc tgaagctggg caatacccgt aaccgcattg acactgccga gcagtttatt
    35281 gataaggttg cttcgtcgtc atcgattacc gggaagccgt atattgtgaa gatccccggt
    35341 aagagcgatg agaatgcaca gcctttttac atgcgttaat tgcgcagacg gataaaacgg
    35401 tgcctgcgca ataatcttaa atccccgccc cctgactaaa atgctcttcc ccaaacaccc
    35461 cggtggaaag gtagcgatcg ccacgatcgc agatgatcgc taccaccacc gcaccagggt
    35521 tagcttttgc cacccgcagt gctccggcaa ccgcgccgcc ggagctgacg ccacagaata
    35581 ttccttcccg caccgccagt tcgcgcatgg tgttttccgc atcgcgctga tgaatatcca
    35641 gcacctcatc taccagagaa gcgttgaaaa tccccggcag atattccgca ggccagcggc
    35701 gaatgccggg aatgctactg ccctcttccg gctgcaggcc aacaatggtc accggtttgg
    35761 attgttcgcg cataaagcgt gaaacgccgg tgatagtgcc ggttgttccc atgctggaga
    35821 caaaataggt aatgcgcccg ccagtctgct gccagatttc cggcccggta gtggtgtaat
    35881 gggcgtaagg gttatcggga ttattgaact gatcgagcag ctttccttca ccacgatccg
    35941 ccatctccag cgccagatcg cgcgcacctt ccatgccctg ctctttggtg accagaatca
    36001 gttccgcacc ataagcacgc atcgccgcac ggcgttcctg gctcatgttg tcgggcatca
    36061 gcaatttcat gcgatagcct ttaagcgcgg caatcattgc cagcgcaatg ccggtgttac
    36121 cactggtggc ttcgattaac acatcacccg gtttaatttc cccgcgcttt tccgcctcga
    36181 cgatcatcga aagcgccgca cgatctttta ccgaacctgc cgggttattg ccttccagtt
    36241 ttaaccacac ttcactgccg ttattcggcc ccattcgctg caacttcacc agaggcgtat
    36301 tgcctattgt ttgttctaat gtactcacga tctctatcca tacgtggtgt tgcctgatgc
    36361 gacgcttgcg cgtcttatca ggcctacagg ttacaaacct tgccattaaa aagcccggat
    36421 cgcgggaagc gcctccgggc gtttaacatt cactcaacct atcaggcgct ttgtgcgaga
    36481 gcaagttcct catctcgggg ttcgatacgc tcgtcgccgt tatacagccg cgcatgttgc
    36541 agaccaacga ataaacgctc gccacgctgc ggggcatcgt cgccatgcat cacgaccgtc
    36601 agcggttcgt tgtaccaccc cagcggctgc accactaatt gggtgtagtg acctttcggg
    36661 ctggcttcca gtacctgtac cggcagcggc gaatcgaggc tggtacggcg gctgatatcc
    36721 acttcccacg ggcgcaggaa gagatccacc ggcccctgat acgcaggtgt gtagcccagc
    36781 ggccagcgat gtgcgccaac atggaactgc ccgccgcgaa tggttccctg caggcggttc
    36841 acttcgccca taaattcgag cacaaaacgg gtcgccggtt cgcgccatac ctgatccggc
    36901 gcgtcagcct gttcaatatt gccctggctc atcacaacta cacgatcagc tacttcggtc
    36961 gcttcttcct gatcgtgggt cacaaaaacg ctggtgaatt ttagttcttc atggagttga
    37021 cgcagccatc gacgcagctc tttacgcacc tgcgcatcca gcgcgccaaa cggttcatca
    37081 agcagcagaa tttgcggttc tacagcaagc gcacgcgcca gcgctacacg ctgtttctgg
    37141 ccgccggaaa gctgcgccgg ataacgatcc gccagatggg caagctggac catttccagc
    37201 aattttgtca ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg cggcagcacc
    37261 gtcaggccaa aagcgatatt gtcgaacacc gtcatatggc ggaacagcgc gtaatgctgg
    37321 aacacgaaac cgactttacg atcacgtgcg tgcaggcggc tcacgtcggt gccgtggaag
    37381 cgaatatgcc cgctggtttg atgctccagc ccagcgataa tgcgcagcag cgtggttttc
    37441 ccggaaccgg acggccccag caacgcgacc atctgacctg aaggaatatc cagtgagata
    37501 tcgttcagca cctgggtgcg accaaacgac ttcttaatat tggcaatctc aatgctcatg
    37561 attttcctcc tgctgcgcgc gtttttcctg attctccagg cgccactgca acatactctt
    37621 taaaaacagg gtgataatcg ccatcagcgt caacagcgcg gcggcggtaa aggagccgac
    37681 tgtgttgtag tcctgctcca gcaattcaat ctgtaacggc agcgaaagtg tttcgccacg
    37741 aatcgagccg gaaaccaccg acaccgcgcc aaactcgcca atcgcgcggg cgttggtcag
    37801 cactacgcca taaagcagcg cccagcggat gttcggcaat gtgacacggc ggaacatctg
    37861 ccagccggat gcgccaagca aaatcgccgc ttcgtcttcc tgactgccct ggcttaacat
    37921 caccggcacc agttcgcgca ccacaaacgg acacgtcacg aagatcgtga ccagcaccat
    37981 tccgggccag gagaacataa tttgcagatt atgttcatcc agccagccgc cgagcgggcc
    38041 gttggagccg tagaacagca gatacaccag accggcaacc accggtgaca cggcaaaagg
    38101 aatgtccagt agcgtcagca gtaactggcg tccggggaag ttaaagcgcg tcaccagcca
    38161 ggccagcaga ataccgaaca ccaggtttac cggtacggca atcagcgcga tcatcaccgt
    38221 cagccagatg gcgtgcagca tatccggatc ggccagattc tgtaaaaccg gcatcagccc
    38281 tttgctgaac gcctgtacga agatataaat catcggcacc agcaggatga acgccgatac
    38341 cagcatcccg atgccaatca gaaaccattt gccccagtta atcgggcgcg cgtcataacg
    38401 cttcaattgt gtcacttccg ccattaatga cctaccacac gccgaccaaa gcgactttgc
    38461 agagtgttaa ttgagaacag cagcagcaga gatgccgcga ggatcaccga agcaatcgcg
    38521 ctcgccgccg ggtaatcaaa ctcctgtaag cgaacgaaaa tcatcagcga cgtcacttcc
    38581 gtcttccaag cgatgttccc ggcgataaat atcaccgcgc caaactcacc aagactacgg
    38641 gtaaacgaca acgccacgcc cgccaccagc gccggagaaa gttccggcag caccactttg
    38701 cagaaactct gccagcgcgt tgcgccaagc gtttctgccg cttcttcata ttccgggcct
    38761 aactcttcca gcaccggctg cacggtacgt accacaaacg gaatgctggt aaaggccatt
    38821 gccaccgcaa tccccagcca ggtataggtg actttgatat caaacttcgc cagccattca
    38881 ccgtaaaaac cgtttacaga aaagagcgag gccagcgtta aaccggcgac tgccgttggc
    38941 agcgcaaagg gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa gcgataacgg
    39001 gttaaaatcc acgccatcag caggccgaaa acgccgttaa aaattgatgc cacaaacgcc
    39061 gacagcagcg tcactttgta ggccgcgacc acctgcgggt tggtgatcac ttcccagtac
    39121 tgcgcccagc tcatctcaga aagctgcatc accagcgcgg agagtggcag cagcaatatc
    39181 aggcacacaa acagcagact ggtgccgagg cttaaggtaa agcccggcag gacgcgtctt
    39241 gatgaaactg caaacattaa ttacgccccg ccgctaacag cttgtctaac tcgccgccgc
    39301 tggtaaagtg ggttttcatc acttccggcc aggagccaaa tttgtcttcc acgcggaaca
    39361 gttcggtctg cgggaattta tctttcagtt tgtccatgac ttccgggtta ttcacgcggt
    39421 agtaatagtc ggtgatgatg gtttgcgcct gcgggctgta aagccagttc agataggctt
    39481 ttgctgcttt ttcggtaccg ttggcttgca cgtttttatc gacccacgcc accgggaatt
    39541 ccgccagaat gttggttttc ggaatcacca cttcaaagcc ctgcgcttcg tactgtttgc
    39601 ggatgttgtt cacttccgat tcaaagctaa tcagtacatc gcccaggccg cgctcggcaa
    39661 aggtggtggt cgcgccacga ccgccggtat cgaacacttc aacgtttttc aggaactgag
    39721 tcataaactg ttcggttttg gctttgtcgc caccgtcagc tttatccgct gcgccccatg
    39781 ccgccagata ggtataacgc gcgttacccg acgttttcgg gttcgggaaa atcagcttca
    39841 cgtcggagcg caccaggtcg ttccagtcgt ggatattctt cgggttgccc ttacggacca
    39901 ggaagcccat ggtggagtag aacggcgagc tgttattcgg caggcgcgac tgccagtccg
    39961 ccgggataag cttgccttta tcgtgcagaa tttgtacgtc ggtcacctgg ttataagtga
    40021 caacgtcggc ttttaagccc tgcaaaattg ccagcgcctg ttttgatgac ccggcatgag
    40081 attgttttat cgtcagtttg tcgccgccgt tatctttggc ccattgctgt tcaaacggcg
    40141 ggttcagggc ggcaaacagc tcgcgggaga cgtcataaga actgttcagc agttccgttg
    40201 cctgtacatg gcccgccagc agcagagaag cgaccagcgc gagtgagttc tttttcagta
    40261 agttaacggc cattgcgcac ccttataaat ttaatgactt tctaatagcc atcatattta
    40321 taacggacgt gaaaggagta acggttttat ataccgtttg gtgatttgga agttgaaaag
    40381 ggaataagac gaaatcctcc ccacaaatgc agggaggaaa cagaggtgaa tcagataccg
    40441 acgctaaccg tctccggcag tgtgctgccg ccatcaatca cattctgtgt accggttaaa
    40501 tagctggatt catccgatgc gaggaaggcc gccagttcgc cgacttccag cggatcggcg
    40561 aggcgacgca gcgggattgc ttttgccatt tcagtcagca ccgactctgg atcttccggg
    40621 ttcgactggc gggcaatgct ttccgccatt ggtgtgcgaa cgtatccagg gcatatggcg
    40681 ttaacgcgaa taccagactg cgcatactcc accgccagcg attttgtcag gccaacaatc
    40741 gccgctttcg ttaaggcgta cgccgtttca cccggatcgg ccaccatatc accagtgact
    40801 gaagacatca tcacaatgcg accatctttg cgggcaatca tctccggcag caccgccttc
    40861 gtgacgttcc atacgccttt aatattgatg tcaatatgga aatcgcgatc gtcatcgctc
    40921 atatcgagga aactgcccag acgacaaacg cctgcgttat tcaccaggat atcaatgcgc
    40981 ccttcttttt ccttcgcgcg tttgatagct gcggctaccg acgccgggtc acgcacatcg
    41041 gcgacaaccg ccgtacagcg atgaccacga ccacacagtt cgtccgccag cttttcgatc
    41101 tcaggggaga tatccagcaa gattaggttt gcgccatgac gcgcaaaagt tctggcaatt
    41161 ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg tcttgcccgt gagtttaccc
    41221 attttaatgc tctccttgtt aatacagagc gttacactcc attccctctg aaaatacagg
    41281 ataaacaaaa tattactgcg gatcagctca ctaattcagg aaaatctcaa tccagtcgct
    41341 gctttatgca tcatttcagg cgttgtgtta gctcactgct gcgctgaatc attttcaatg
    41401 actccacatc attgagttgc acaagaccaa caaacagtaa atcggtaaca gaattttgcg
    41461 ccgtgcgggt agacatcgac gagctacgcc attcggtttc accggaaacg gtatcgagcg
    41521 tgaaatgcgc cagccgccgc agcggagagt cagccagaga agtaatggca atcaccgttg
    41581 ccccctgctt tcgtgccgct tctgcgcaca agacaatctc ttttttactg ccgctgtaag
    41641 agatagcgat ttgtacatcg ccttttttca acgcctggga aacggtggct tgtacgtgcg
    41701 tgtcggcttc gcaggccacg cgatatccaa ttttcatcag tttgaacgac agatcgcacc
    41761 ccaccagcgc cgatccgccc aggcccgtta tctggataaa cggtgcttta ctgatgacct
    41821 caatgatttt ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc agcgccagct
    41881 ctttttcgcg attcaatttg cgggcaatca cttccagcga atcatcactg gtaatcgaac
    41941 tgtgcaggtg ctgcgccgtg gcattcgttt tctcccggct ggcactgtat tcgcctatta
    42001 acgccatacg caattcagta aacccttgtg cgccgagctt ctgggcaaac ttcacaatac
    42061 tcgactggct gatgccgagc tgtttcgcca tcttgcgcga agagacggat ttcagctcac
    42121 tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt ctcggtaaat tcactttccg
    42181 cattgcgtat tttcgtcagg tacaacatct tccactctcc tgggctggca gcaaaaattc
    42241 cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt agactcaatg attcctttaa
    42301 gtctgtcaat tcgggatata tgattccata catctgcaat tatttgatcg taaatagtaa
    42361 ggtcaccacc gatgcaactt gaaaagatga ttaccgaagg ctcgaacgcc gcctcggctg
    42421 aaattgaccg cgtatcaacg ctggaaatgt gccggattat caacgatgaa gataaaaccg
    42481 taccgcttgc cgttgagcgc gtactgccgg atatcgccgc ggcaatcgat gttatccacg
    42541 cccaggtaag cggcggcggt cgtctgattt atctcggtgc gggaacatcc ggtcgtctgg
    42601 ggattctgga tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt ctggtagttg
    42661 gattaattgc tggcggcgaa tatgccattc agcacgcggt ggaaggcgct gaagatagcc
    42721 gggaaggcgg tgttaatgat ttgaaaaaca tcggattaac cgcgcaggat gtggtggttg
    42781 gcattgctgc cagcggtcgc acgccgtatg tgattgccgg actggaatat gcacgccagc
    42841 tcggctgccg tacagtggga atttcctgta atccggggag cgccgtttcc accaccgctg
    42901 agtttgctat tacgccggtg gttggggctg aagtggtcac cggttcttcg cggatgaaag
    42961 caggcacggc gcagaaactg gtgctcaata tgctttccac cgggctgatg attaaatccg
    43021 gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa ctgcatgtgc
    43081 gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc cgaacaagca gaagcagcgt
    43141 taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa aatctcgatg
    43201 ccgcagaagc taaaaaacgc ctggatcaac acggcggctt tattcgtcag gttttagaca
    43261 aggaataacc catggccaaa gagatcagca gtgaacttct gaacaccatt cttacccgtg
    43321 tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg
    43381 tacatgacag ttcactggtt gatcccgata tcaaaacgct ggaaggcgtg aagggcgtca
    43441 ttttgaccag tgaccaggtg caggtcgttt ttgggcctgg taaagcgcat cgtgccgcaa
    43501 aagcgatgag cgagctgctg ggagaagcac cggtacagga tgccgcagaa atcgccgccc
    43561 agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt ctcgccaaat
    43621 tcgccaccat cttcacgccg cttatccccg gttttattgc cgccggtctg ctgctgggga
    43681 tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc acgctacccg
    43741 acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt caccttcctg gtgattctgg
    43801 tgggctataa cgccgcccag gcatttggcg gcacgggcgt caatggcgca attattgccg
    43861 cactcttttt gctcggttat aaccccgctg caaccaccgg ttactacgcc ggttttcacg
    43921 atttctttgg tctgcccatc gatccgcgcg gcaatattat cggcgtgttg attgccgcgt
    43981 gggcctgcgc ccgcatcgaa ggcatggtgc gccgctttat gcccgacgat ctcgacatgc
    44041 tgctgacctc gttaatcacc ctactgatca ccgctacgct ggcctatctg atcattatgc
    44101 cgctgggcgg ctggttattc gaaggtatgt cgtggctgtt tatgcacctg aacagtaatc
    44161 cgctcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt ggcgtgcatc
    44221 agggctttat tcctgtttac ctcgcgttaa tggacagcca aggattcaac agcttatttc
    44281 ccatcctttc gatggcaggc gcgggccagg tgggcgcggc gctggcactc tactggcggg
    44341 cgcaaccgca cagtgcatta cgcagtcagg tacgcggggc gattattccc ggcctgctgg
    44401 gcgttggcga accgttgatt tatggcgtca ccctgccccg catgaaaccg tttattaccg
    44461 cctgtttagg cggcgcggcg ggcggtttgt ttatcggatt gatagcctgg tggggtctgc
    44521 cgatgggctt aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg ctgatgactt
    44581 cagcacaagg cattcttccg gcgatggcag tctacgctgg cgggattctg gtggcatggg
    44641 tttgcgggtt tattttcacc acgctttttg gctgccgtaa cgtcaatctg gactgatatc
    44701 atgaaacgga caatgctcta tctttctctg ctggctgtta gttgtagcgt cagcgccgca
    44761 aaataccctg ttctgacaga aagttcgcca gagaaagcag ggttcaacgt cgaacggctt
    44821 aaccagatgg atcgctggat tagccagcaa gtggacgccg gttatcccgg cgtaaacctg
    44881 ctgattataa aagataatca gattgtgtat cgcaaagcct ggggagcggc gaaaaagtac
    44941 gatggcagcg tattgatggc gcagcctgta aaagctacca ccgggacgct gtatgacctg
    45001 gcctcaaaca ccaaaatgta tgccactaac ttcgctctgc aaaagctaat gagcgaaggc
    45061 aaactgcatc ctgacgatct gattgcgaaa tatatttcgg ggtttgccga tagtccaaat
    45121 gacaccatca aaggcaaaaa cacgctgcgg atttctgacc tgctgcatca cagcggcggt
    45181 ttcccggcgg acccacaata tccgaataaa gcggtcgcgg gcgcgttata ttcccaggat
    45241 aaaggccaga cgctggagat gatcaagcgc acgccgctag agtatcagcc cggcagcaag
    45301 catatttaca gtgatgtcga ttatatgctg cttggattta tcgtcgagtc ggtcaccggt
    45361 cagccgcttg accgctatgt tgaagagtcg atttatcgcc cgctcggcct gacacatacg
    45421 gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa ttgccgccac ggaattgaac
    45481 ggcaataccc gcgatggagt gatccacttc ccgaatatcc gcacctccac tcactggggt
    45541 caggtgcacg atgaaaaagc cttttattcg atgggcggcg tttccgggca cgcaggtttg
    45601 ttttccaata ccggcgatat tgcggtgtta atgcaaacga tgctgaacgg cgggggctat
    45661 ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt tcaccaccag ctctaaggaa
    45721 gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg ccaccatgac gccgacgttt
    45781 ggcacgctgg caagcccgca aacctacggt cacactggct ggaccggaac ggtgaccgtt
    45841 atcgatccgg tgaatcatat ggcgattgtg atgttaagca acaagccaca ttcgccggtt
    45901 gccgatccgc aaaagaatcc caatatgttc gaaagcggtc agctgccgat tgcaacgtat
    45961 ggttgggtag ttgatcaggt gtatgcggcg ttaaagcaaa agtaataaaa aggccagtca
    46021 gtaatgttac tgcctggcct ttaatcaatg aatcagaaac gcttacagcg ccatcaactt
    46081 gtccagcgac ggtgcgaaat aatagccgcc ggttaccggt ttggtgaaac gcaacatcgc
    46141 atcacgctta ccatcggtat cgccaaacat gctcagcagt tgctgctcaa tgttatgcag
    46201 acgcgcgcag taggcgcaga agtacagacc gtgagtgcca ctggcggtgc cgtacggcag
    46261 gctctggcga acaatcttca gccctttgcc atcttctttc agatcaacgc gggtcaggtg
    46321 agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg gcctctttgg tgcgcccgat
    46381 catcatctcc tgatcgtgaa cgctcatccg gttgagctgc ttcaggttgt gctcccaacg
    46441 ctgcacaaac acatagctgc cgccggcatc cacgccgtct ttgataaccg ccacttcgcg
    46501 acgcgtctct tcacccgccg ggttttctgt gccgtcaaca aagccgctca ggtcacgctc
    46561 ttccacccaa cggaagccgt ggatctcttc tttcacttca atgcagtcac caaaggcttc
    46621 catcgccgcc tgggcgacag agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa
    46681 cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag cccggaaaat ctttcagctc
    46741 ttctgcccca acgccgccgc tcagagcgcg ccagatgttg ttaccaaagg caaccaccgc
    46801 accaagatgc gcgtccggga atttcgcttc aaaagttgcc agtttgtcgg caaatgtttt
    46861 actggccgca cgcagggcgt caacttcccc tttcacgttg gcttcgatcc aaatcgccgc
    46921 gcggcaatgt tctggcaaaa tgccactctg aacctgagac attgttcctc ctgaaaataa
    46981 taatgccacg caatccgtgg cattgttgag cgctatttta cgtgtttttt cgcggcggcg
    47041 gtttgttcag acgcaaatta acgccgccag ataattttac tgactttcca gtttttcagg
    47101 gtatcgtccg aaggcattaa cccttcagga ccactccatt ccccgctgaa ctggtagcta
    47161 atatgttgac tgccttcggc tttacattcg acggcacgat tatcatcacc atcagctttt
    47221 tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg
    47281 gtgtcagcag gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg
    47341 ttaatcacca tcgccacgtt gtcgcccttc atcacttcaa aaaagcgcac cacgttgccg
    47401 ttcgcggttt tcattccgct gcgcaggcga taatcgccat caaccgcatc ggcaatggct
    47461 tgttcttgca gtggtgtgga cgccgttaat tcaccaacgc cctgctcgct cactttggtg
    47521 gacgacccaa accagttcca cgggttagcg gcagaccagt taactgacga catcgtggaa
    47581 cagccggtca gcatcaatgg catagcgcat aacattaaac gcagcgattt catgtcactt
    47641 cctttggtta ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg ccgttattct
    47701 tttacttcat taaaacaagc gcgtaaacgt cgattggtca gcagccagat taacgccaca
    47761 atatccgcca ctaccagcgc cagaccgata ccgctaacgg attcaccgtt cagccacagc
    47821 cacggttgcc agcaaagtaa aaccacctgc gccaacagca acagaaaata gagtacacgc
    47881 caggtgcgag gaaacgtagc ccgccgaccg ctcaacaaaa acgccagcac cgccggaatg
    47941 ccaggaatca gccccagcca gaaattatcg tgatcgggat aaaacagatt tagcagcgca
    48001 gtaccctgct cgcgcgacgc accggcaatg acaaacagca cccaggttcg cgcctgaagc
    48061 aatagcacaa gccagaagag caagggtaaa cgcaggcgac cgtgcgcatc ataatggaca
    48121 ggatgaaact cagtactctt catcttcaat caaacgctta cccagactca gcacgtcggc
    48181 gtgttcatat cccaggcgtt catacattcc gagcaccatg tcgttatctt ccggcacgtt
    48241 gatctgaatt ttcgggcagc cgcgagcaat cagctttttc tccagccggt taagcaacgc
    48301 attggcaatc ccacgcccac gaaactctgg atgcacgcca agataataag cagacccgcg
    48361 atgcccgtca taaccgccca tcaccgttcc gaccacttca ccgttcacct cagcgaccag
    48421 aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc atttccggat cgttccacgg
    48481 acgcagcaag tcgcaacgct cccaaagggt gatgacctct tcgaaatctt cctggcgaaa
    48541 tacgcgtatc tccatggtat tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg
    48601 aacggtcgaa ttagccaata tctgacgaaa atcggttgaa aaagtggcat aatggggagt
    48661 tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacag caaacggtcg taacggatta
    48721 cgcgatacga tataacatct ggaactttat tattacaact caggccgtat gagcactttt
    48781 aaaccactaa aaacactcac ttcgcgccgc caggtgctga aagccggttt ggctgccctg
    48841 acgttgtcag gaatgtcgca agccatcgcc aaagacgaac ctttaaaaac cagcaacgga
    48901 cacagcaagc cgaaagctaa aaaatctggc ggcaaacgtg tcgttgttct cgatccaggc
    48961 cacggcggga ttgataccgg agcgatcggg cgcaacggtt cgaaagaaaa acatgtggtg
    49021 ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc atgggattga tgcgcgttta
    49081 acgcgttctg gcgatacgtt tatcccactt tacgatcgcg ttgaaatcgc ccataaacat
    49141 ggcgcagatc tgtttatgtc aattcatgcc gatggcttta ccaacccgaa agctgccggt
    49201 gcttcggtat ttgccctctc taaccgtggg gcaagtagcg caatggcgaa atacctgtct
    49261 gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa ggatcatcta
    49321 ttgcagcaag tgctgtttga tctggtgcaa accgatacta ttaaaaacag cctgacgctc
    49381 ggctcgcata ttctgaaaaa gattaagccg gtgcataaac tgcacagccg caacaccgaa
    49441 caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg
    49501 tttatcacca acccggaaga agagcgactg ttaggcacgg cggcgtttcg tcagaaaatc
    49561 gccacagcga ttgctgaagg cgtgatcagt tatttccact ggttcgacaa ccagaaagca
    49621 cattcgaaaa agcgataagt tatgaaaccc gacgcacacc aggttaaaca gtttctgctc
    49681 aaccttcagg atacgatttg tcagcagctg accgccgtcg atggcgcaga atttgtcgaa
    49741 gatagctggc agcgcgaagg tggcggcggc gggcgtagtc gggtgttgcg taatggtggt
    49801 gttttcgaac aggcaggcgt caacttttct cacgtccacg gcgaggcgat gcctgcttcc
    49861 gccaccgctc atcgcccgga acttgccggg cgcagtttcg aggcgatggg cgtttctctg
    49921 gtagtgcatc cgcataatcc gtatgttccc accagccacg cgaatgtgcg cttttttatt
    49981 gccgaaaaac cgggtgcaga gccagtctgg tggtttggcg gcggcttcga tttaacccct
    50041 ttctatggtt ttgaagaaga cgccattcac tggcaccgca ccgcccgtga cctgtgcctg
    50101 ccatttggta aagacgttta tccccgttac aaaaagtggt gcgacgatta cttctacctc
    50161 aaacatcgca acgaacagcg cggtattggc gggctgttct ttgatgatct aaacacgcca
    50221 gatttcgacc actgttttgc ctttatgcag gcggtaggca aaggctacac cgacgcttat
    50281 ttaccaattg tagagcgacg taaagcgatg gcctacggcg agcgcgagcg caattttcag
    50341 ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct gggatcgcgg cactctgttt
    50401 ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt caatgccgcc actggtacgc
    50461 tgggagtatg attatcagcc aaaagatggc agcccagaag cggcgttaag tgagtttatt
    50521 aaggtcaggg attgggtgta actccctcac ccccattccc gcatccgctg atgcaacgtc
    50581 aacgacggct tctcggcaaa cagctgctgg taatccgtgg caaattgccc cagatgccag
    50641 aatccccact gcatggcggc gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt
    50701 tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg cgttcgggcc aattcctaaa
    50761 atagcgtgaa acgcgttttg tagcgtgcgg cggctgacat gcagttgatt acacaagtcc
    50821 agcaccgtca ccggttcgga catgttttcc agcacatatt cacgggcgcg ggaaagcaac
    50881 cgacggtaac tctgatgact gatgctttcc gccgtcacca ttggctgcgc ttcttccagc
    50941 atcgccccca tcgccattag caaattatcc cccagcactt ttcgcactgc aggctgatgg
    51001 agattttccg ggttctcgca aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg
    51061 gctttatgct gctctttcac ttccagcgcc gactggctac gcaacatatg taatacccga
    51121 tccggattat gcaaaaagtt agcctgacgg gtgatgacat cttcagaaag cactacgccc
    51181 aggatcgtgt aatcgtccgg cgtacttaat tcaaactcgg ttccaccagg gcgcgtcgct
    51241 atttctgcgc ttcccagaca ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga
    51301 atgccaaacc agaacgagtt cggccagacc agacacgact gacgcagcgc cagaccggtg
    51361 tattcacgaa aaacctgaat atcatcgagt agaatttccg taaattcacc atgaaacttg
    51421 cccggatgca gctgatcgta aatctgctgc caggcggtaa tcgttaaagc atgttcatag
    51481 acatccgttg tctgtcgttg atgaacatta tccacttcga ccttcggcgt gagcttcagg
    51541 ttttcgggta agggttcatg ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg
    51601 atgttaatgc cgggtgttgt aggacacccg gcacctccga caggttaatg gggcttgaga
    51661 cgataacgac tactgcgttt acgtaacgtc ccagcagaaa agagctgttc gagcgcattt
    51721 ctggcttttt ccagcggcca gccaaagtgg gcagccactt ctcctgccgt cactccctga
    51781 cgtactgatg ttaacagcgc caacaactcg tcagcagatt ccgcgacgat aactggcgcg
    51841 tcgggttcct ttacgggttg cttcggctgg cggttaaagc cagtaaccag ccactgggtg
    51901 tcatcgtctg gccgaccgat ctccttacgg ctgatgacac gcccggtacg catcgcggca
    51961 gcacaacctg cgtccagcgc agcacgacac gccgccagat cgccttccac caccagcgtt
    52021 aagcgacctg ggtcaagcac ttcgtgactg agcagacgaa cgttagctgc tttgagcatg
    52081 gcatccgctg catctatcgc ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg
    52141 atcattggca acctccgtta cgcacgctgg attggattac gcgcgatttc cagcactgca
    52201 tcggtaaagg cgttacaggc cgctttacac gctgcctggc taccggttaa aaatgctgcc
    52261 gagtagttag tttcagacgg tggcgggaca taggtcacca gctgtacgtc ggcagatttc
    52321 aacgctgcat caatgccgta ggtcgcttcc agcggcggtg ccaccagata cgccatcgga
    52381 tcgccaagcg tgatcccggc ggttgatgag agataagaac cggtacgcga aactacatgt
    52441 gccaggaatg ccgtatcttg cgcatcatta gcccactgga aagcagcgcc attttcaata
    52501 tgcgcaacca tcgcatccag accggcacgc acttccgccg ggtttggccc accaagcata
    52561 atcagcacct caccggcagt cggtgacggg ccgtgtgccg cgcccgcata cagcgagcga
    52621 ccatatacca cttctaccat cgcttgtttg gtcgcttcgt cagccgcaat gtaggttacg
    52681 tcgtcagaat cggcagaaat cagaccgagg ctacgaatat gtggcggcaa tttcagttca
    52741 cgcgcaaaat cggcgttaac ggaggcaatc acccgcatgg cggttaccga cggtcgaatc
    52801 aaatctaaag ctggcatgat gcctccttat cgggtcatgt tgatgccgga tgctttctgc
    52861 tccagcatac gtttcgccaa atccacaatg acggctgcgg cttcaaccgg cggcgtgccg
    52921 ccctggtgaa tgttagagat acaggtgcga tcggcctcaa cggtggtcgc catacgcggc
    52981 gagtaaacgg cgtagcagga gaggctttct gactgaccca accccggacg ttcgcccacc
    53041 agcaggatca ctactttcgc gccgaggatc tcaccgatct gatcttcaat cttcacgcga
    53101 ccataacgaa caaagaatgg cgtgccgact ttcagcccgg cctgtttcag gcccgccatc
    53161 agcggcggca ggatctcttc gtagttcaca gtgatggcat cagttgacag gccatcagaa
    53221 ataacgacct gtacgtccgg attagcaaca cactgtgctt tcagcgcttc aacagcttct
    53281 gcacacaggc ggcggcccat atccgggcga gtcaggtaga gatttttgtc gctaatttcg
    53341 gagcgtactt ccagcaagcc ctgcgctttc acccactctt ccggcacttc tttcagtacg
    53401 gtatctttcg aacgagagtg atcggccagg aaacgcagca gcgccagcgt acgcggacgc
    53461 ggaccggcac gaccggtaca aacgcgggcc acggtgctgc gacgcagttc tgttaacaca
    53521 tccgcacgat gtgggttttc aacaccaatc cacacttttg cttccgcaga acctaaatcc
    53581 agcgcgcagc tttccgaggt caccggtgcc gcacaggtgg tagtggcgca ctttgcttct
    53641 gacggcgcgg gggccgtttg tcccattgac gccatcacgc tgcgtacaat ttcttcaatc
    53701 tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa gaacagtgac ggatcgcccg
    53761 cccgtttggt caggcgaccg tttgccataa tgcccatgct ttccagccag cgttcaaact
    53821 ccggtgacgg gcgcaggttg agtaactgac gcacagtggc ggtatcgtgg aatgcggtgg
    53881 tctggtagtt gagcatgatg tcatcaccca gcggcatccc catgatgtag ttgcagcctg
    53941 cggtggcgag caggatcatc aggttttcgt tgaggttctg gtcagcgtca gcgtggttgg
    54001 tgtaacagca gtcacagccc atagagatgc cgctcagctt gcccataaag tgatcttcta
    54061 agcccgcacg gataatctgg cggtcgttgt agagatactc cggcccaata aagccgacca
    54121 cggtgttgac gataaacgga tcgtaatgac gcgccagccc atagttacgt gcttccatcg
    54181 tcacctggtc tgcgccgaag ttagcgccag cggatagcgc tgaaccttgt ccggtttcga
    54241 agtagaggca gttttccccg gcgatacggt tgaactctgc acccactgcg cgcgcttcgt
    54301 cgagcatcgc cagttccacg ccaaactctt tcagcccttt ttcgctgcca cagatactct
    54361 ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc gatctgggtg gtgacgtgcg
    54421 ccagtacgca gccctgagtt gggatgttga atttgtcgat cacgccatag atggtgtcca
    54481 gcacgcggct taagttttcc acgtcgtcag tcaccgggtt aacgccgatc accgcatcgc
    54541 ccaccccgaa ggaaagccct tcgtagattt gcgcggcgat actttgcacg tcgtcacggg
    54601 tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat accgatggtg gtattggcct
    54661 ttttgattac cggcattttc ttcgcgccgt agatcaggtc cgcgttggag caaatcttcg
    54721 ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt aaaggcaatg tcgtccacgc
    54781 tggtttcatc gctcagcaca tactcacgca gttcgctgat gctccagttt ttaatctggt
    54841 tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt cacgcagtca tcttcatagg
    54901 caatcaccgg attattgcgg atgtccgcta cggtcatttc cgacaacacc tgctttgccg
    54961 ccacgcgctc ctgtgagctt gccgctgcga cgcccgccag cacatccccc gaacgcagtt
    55021 cgttggcttt agccagcacc tcttttacat ccttaaactg atatacattg ccgaacaatg
    55081 tggtctttag tttcataagt cgttccctca ggaaggaaat gcgagtgatt tcaccgtcac
    55141 cggcacaacc gatccgccaa aaagaggcgt accaatgtcg atatagtccc ccgcgcggac
    55201 aatcacttcg tcaatgactg ccaacgggag ttgttgtagc tgtgggcgca acagcatgcc
    55261 cagagcttta ccaaagtcct gcccggccac caccagcagg ggatgcggat tcggaaaacg
    55321 cgcgacgaaa tcgaccaacg cgttgatgac cgtcagtacc gcggcgtaac gcacaggcag
    55381 cgaggcggga agcgccagca cgtacgcgtc agttttggga tcaagatcca gctgaatcag
    55441 cgcctgttgc caggcactca ccagatccgt ttcatcaatc gggatcgcca ccggcaaatt
    55501 gcgcagcggc agttgtacgc cctccagcca gattgtgctg ccagagagcg aaagggtatg
    55561 tgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc ggaaactgca cgttcatctc
    55621 acgcaggcgc ggatggtcat gtagcgccgt cgccagtagc gggccaatat cggcaaaaca
    55681 gaacgggtca gcgggctggt ggcgataaca ttcgcccacg ccgccagaaa gcgtaatgat
    55741 ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatt aatgcctgcg cgagcggcga
    55801 aagcgttccg tcaatcactt cgacaatcag ctctgccatc cgtcgggtaa cctgcaccag
    55861 ctgcgcgccg gtcagcgaac ggacgtcagt gcctgcaccg aaacactcat ccacaatcat
    55921 ctgccccggt ttatgagcgt aaaccacgcg cccctggctg tcggtttcca gcaggcgacc
    55981 accgacgttg aggcaggcag tgccgctgat ttttccggca tcgaacaggg cgtagttcgc
    56041 ggtgccaccg ccgatgtcga tattcagtac ccgacacagc cgttgttcag aaagggtttg
    56101 cgccccggca ccgtgaccgg cgatcacgga ttcgaggtgc ggcccggcgc tggcgacgac
    56161 aaaatcgccc agcgactgag agagcgccat caccgccggg cgagcattgc gggttttcgc
    56221 gctttcaccg gtgatgatga tggcaccaga atcaacgctt tccggcgcaa tgcccgcagc
    56281 ctgatattgc tctaaaatca gcgatttcag ttccgcttct tttaaaccac cctgtttatc
    56341 gacaggggta aagaacaccg ggctttgcca gctaatttcg cgtttgatga attcgtagcg
    56401 cggcacctgc gacaccgccg cacggttaac cagttccagg tgggagaaaa tcacctgggt
    56461 ggtggtggtg ccgatatcga taccgacgct cagtagctgg cgagtgttca cgattgtgcc
    56521 tccgcttcgg ttttagccgc ggtcgcgtcg tcttttggca ccagcatcat cgccacgcca
    56581 atcgccgtta cgccgccgat caacttgccg acaatcatcg ggaagatcat ggcattcatg
    56641 ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag cagcggaaac ggcgaaggcg
    56701 cagttgatga ctttgccgcg ggtatccatc tgcttcatca tgccgaacat cgggatgttg
    56761 ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt tcatattcag cactttaccg
    56821 acgctcatca gcggtttttc aaaccagcgg gtcagcagca gcaccatcgg atacgcccct
    56881 aacagaacgc aggagataga accgataact tcaatggcgc gcatcacctc accgggttta
    56941 tcgccagggg ccataaagat aggatccaga ccggggatca gttcccagcc aagcaggaat
    57001 ttcactaccg cagcggcaag accgagggtg atcaatgcaa cgaggaattt ggcgaagatc
    57061 tggaagccgt tgatcatttt ttctgggatg aatttcagcc ccagcgccac cagaatcgca
    57121 acaatgatca ccgggatcat gttcatcagg atcagggcga aagtgaattc caccggctgg
    57181 ccgttgatct gaacaccgga gtacatagca accagaccac cagcgataca accaatcgga
    57241 atggtcacaa tgcccgccag cacgccgagc gccagataac gacggtcaga aggttcgata
    57301 atgccgagcg ccaccggaat ggaaaacaca atcgttggcc ccatcatcga cccgagaatt
    57361 aacccagagt atagccacgc ggctacgtcg ccgcccgcca gctctttggc gaggaagaag
    57421 ccgcccatat cgcacgccag cagtgttccg gcgaacatcg atgggttagc gccgagcatt
    57481 tcgtaaaccg gaataattac cggcccgagt acgtgagcca gtaccggtgc cagcgcggtc
    57541 ataccgacca tcgccaggcc cagtgcgccc attgccataa agccttcttc gaactgaccg
    57601 ccggatcctt cgatactttt accgaactta ccgaggaaac gagcagaacc gccgaactgt
    57661 gacaggatcc tgtcaacggc agctatcagc ataaagaaca tcatgatgta catgatgatt
    57721 tcgttaattc ccatcgcctt tactccctgt tagttgttat ttattagcgg atgctgcgtg
    57781 aagccttatc cgcactacat gtgcaatccc acagaccgga taagacgcgg catttattgc
    57841 gccgctgcgt acaggccgac aagctgttcc aggctggcgg tacgcgggtt gctgcgcaga
    57901 caaatatctt ccagcgcggc ctgcgcccat gcaccgtaat gcgcagatgt cgcgccaaca
    57961 tcgcccagtc gtttaccaat cccaacttcc gcaatcagct cacttaccgc gttaatagcg
    58021 tcacgatcgt cggatttttt agttcgcagt gcccgaccaa tctgactaaa gcgttcgcga
    58081 caaaccatcc ggttaaactc catcaccgtt ggcagcaaca tagcgttcgc gagaccgtgc
    58141 ggaatatgca gcgccgcgcc cggctgatgc gccatcgcgt ggcacaaccc aagacccgca
    58201 ctggaaaacg ccattcccgc catacatgaa gccagcaaca tgctctcgcg cgcggcaagg
    58261 tcgtgaccgt agcccaccgc tttcggcagc gatttgccaa tcatcgcaat ggcaccaatc
    58321 gccagactgt cggtaaacgg tgtagcgttc agggcgctgt atgcttcaat ggcatgggtt
    58381 aacgcatcaa tgccggtcat cgccgtgaca tgcgacggca caccttcggt caatgcggcg
    58441 tcgaggatcg ccacatccgg catcagcgag gcatgggcta acacctgctt gcgcccgctc
    58501 accgcgtcga taatcaccgt tacattggtg gtttcagagc cggttccggc ggtagttgga
    58561 atggcaatca gcggcaagcg cggttgcaga acgctggttt ctgacatctc tgccagcgtg
    58621 ctatccgggt tcgtcaccag caacgccacg gctttcgccg catccagcac cgagccgccg
    58681 ccaaatgcga tcaccccgtc acaacctgac tcacgcaact gcgccacggc tgcacacacg
    58741 tcggtaatgc acggttcgcc caccggacat ggccagagcg tcatggcgat acctttaacg
    58801 gccaggctac gcgtcagccc ggcggtcatc cctgcctgat gcaaaaagct gtctgccatc
    58861 acgaacagat gtttcagccc acgcgtttgc gcttgctgtc cgcaactgct caccgagccc
    58921 ggaccgcaaa gcgtcaccgg tggaacgcta aatgttttta cccgttgcag attcagggta
    58981 tcgaacgcct gaaagagcgc ggtctgcaat tcattttgca tatagcccct ccgctttctc
    59041 tcgcccacta ctggcgatcg ccagcggggt cataaacaaa ctgtgctgcg gtaaatgcac
    59101 ctgtaacgcc gggaattgtt tgcgaaacag ttctgccacg cccggttgca tacaggagcc
    59161 gcccgccagc cataaatcag taatgccctg cccttcaata tgccgggcga caatgtccgc
    59221 cattttttcg tacaccggtt tcaccgtagg ccaaatctct tcaccgtgac cgcgcttgta
    59281 ctgctccgcc tcttccagcg aaatacggcg atttccggcg aaagtcagag agatgtgatg
    59341 tccgccggtg gcttcatccg ccgagtacgt cactttgccc tttttcacga tggcaatgcc
    59401 ggtagtgcca ccgccgatat ccactacacc ggcgttgtcc agttgcagca gatccgcgac
    59461 tgcggttggc tcatccagca catggctcac ttccagcccg gcagactcca gcacgttgat
    59521 ggaaatacgc gggtccgtac ctggaggaaa tgaagtcgcc gcatggctaa aacgacgacc
    59581 gaattgctgc tcgagcgtgt cgagatggcg acgaacaatg gtgaccgcgc cgaagaaatc
    59641 ccagacgatg ccgtcgcgaa ccacatcggc ccagtcgagg cataccgcca ctggctgacc
    59701 gtcgcggtcg acaaccatcg acaccacatc gcaggtgccc agatccactc ccagccacag
    59761 cggagattcc gtcgcggcgg gcgtctggtt acacagcgtt gccgccgttt gcagacgtgg
    59821 ggtgagccat tgttcgtcgt gcgccatctg ttactcctta aacaatgcga aacgcatcga
    59881 ctaatacaca gcgacgcaga cggacaaacg tacgcgcgct ggtcacccct tcaccggttg
    59941 gcgtggtgat ggtcatggtg gtcc
//
LOCUS       DAZKFZ010000007        58527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000007 DAZKFZ010000000
VERSION     DAZKFZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            134..2377
                     /gene="pdeF"
                     /locus_tag="ACHOQW_000417"
     CDS             134..2377
                     /gene="pdeF"
                     /locus_tag="ACHOQW_000417"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP9571549.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2416..3957)
                     /gene="ppx"
                     /locus_tag="ACHOQW_000418"
     CDS             complement(2416..3957)
                     /gene="ppx"
                     /locus_tag="ACHOQW_000418"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP9571550.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3962..6028)
                     /gene="ppk1"
                     /locus_tag="ACHOQW_000419"
     CDS             complement(3962..6028)
                     /gene="ppk1"
                     /locus_tag="ACHOQW_000419"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP9571551.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6199..6837)
                     /gene="purN"
                     /locus_tag="ACHOQW_000420"
     CDS             complement(6199..6837)
                     /gene="purN"
                     /locus_tag="ACHOQW_000420"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9571552.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6837..7874)
                     /gene="purM"
                     /locus_tag="ACHOQW_000421"
     CDS             complement(6837..7874)
                     /gene="purM"
                     /locus_tag="ACHOQW_000421"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9571553.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8199..8825
                     /gene="upp"
                     /locus_tag="ACHOQW_000422"
     CDS             8199..8825
                     /gene="upp"
                     /locus_tag="ACHOQW_000422"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9571554.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8911..10200
                     /gene="uraA"
                     /locus_tag="ACHOQW_000423"
     CDS             8911..10200
                     /gene="uraA"
                     /locus_tag="ACHOQW_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP9571555.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10295..10996
                     /gene="hda"
                     /locus_tag="ACHOQW_000424"
     CDS             10295..10996
                     /gene="hda"
                     /locus_tag="ACHOQW_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP9571556.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11134..11493)
                     /gene="arsC"
                     /locus_tag="ACHOQW_000425"
     CDS             complement(11134..11493)
                     /gene="arsC"
                     /locus_tag="ACHOQW_000425"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP9571557.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11514..12977)
                     /gene="bepA"
                     /locus_tag="ACHOQW_000426"
     CDS             complement(11514..12977)
                     /gene="bepA"
                     /locus_tag="ACHOQW_000426"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP9571558.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13190..14251
                     /gene="yfgO"
                     /locus_tag="ACHOQW_000427"
     CDS             13190..14251
                     /gene="yfgO"
                     /locus_tag="ACHOQW_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9571559.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14289..15137)
                     /gene="focB"
                     /locus_tag="ACHOQW_000428"
     CDS             complement(14289..15137)
                     /gene="focB"
                     /locus_tag="ACHOQW_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP9571560.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15159..17171)
                     /gene="hyfR"
                     /locus_tag="ACHOQW_000429"
     CDS             complement(15159..17171)
                     /gene="hyfR"
                     /locus_tag="ACHOQW_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP9571561.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17201..17614)
                     /gene="hyfJ"
                     /locus_tag="ACHOQW_000430"
     CDS             complement(17201..17614)
                     /gene="hyfJ"
                     /locus_tag="ACHOQW_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP9571562.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(17607..18365)
                     /gene="hyfI"
                     /locus_tag="ACHOQW_000431"
     CDS             complement(17607..18365)
                     /gene="hyfI"
                     /locus_tag="ACHOQW_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFP9571563.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18362..18907)
                     /gene="hyfH"
                     /locus_tag="ACHOQW_000432"
     CDS             complement(18362..18907)
                     /gene="hyfH"
                     /locus_tag="ACHOQW_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP9571564.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18917..20632)
                     /gene="hyfG"
                     /locus_tag="ACHOQW_000433"
     CDS             complement(18917..20632)
                     /gene="hyfG"
                     /locus_tag="ACHOQW_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP9571565.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20622..22202)
                     /gene="hyfF"
                     /locus_tag="ACHOQW_000434"
     CDS             complement(20622..22202)
                     /gene="hyfF"
                     /locus_tag="ACHOQW_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP9571566.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGRP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22207..22857)
                     /gene="hyfE"
                     /locus_tag="ACHOQW_000435"
     CDS             complement(22207..22857)
                     /gene="hyfE"
                     /locus_tag="ACHOQW_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP9571567.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22869..24308)
                     /gene="hyfD"
                     /locus_tag="ACHOQW_000436"
     CDS             complement(22869..24308)
                     /gene="hyfD"
                     /locus_tag="ACHOQW_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFP9571568.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24325..25272)
                     /gene="hyfC"
                     /locus_tag="ACHOQW_000437"
     CDS             complement(24325..25272)
                     /gene="hyfC"
                     /locus_tag="ACHOQW_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP9571569.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25283..27301)
                     /gene="hyfB"
                     /locus_tag="ACHOQW_000438"
     CDS             complement(25283..27301)
                     /gene="hyfB"
                     /locus_tag="ACHOQW_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFP9571570.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27301..27918)
                     /gene="hyfA"
                     /locus_tag="ACHOQW_000439"
     CDS             complement(27301..27918)
                     /gene="hyfA"
                     /locus_tag="ACHOQW_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP9571571.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(28171..28641)
                     /gene="bcp"
                     /locus_tag="ACHOQW_000440"
     CDS             complement(28171..28641)
                     /gene="bcp"
                     /locus_tag="ACHOQW_000440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP9571572.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28641..29213)
                     /gene="gcvR"
                     /locus_tag="ACHOQW_000441"
     CDS             complement(28641..29213)
                     /gene="gcvR"
                     /locus_tag="ACHOQW_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP9571573.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29359..30237
                     /gene="dapA"
                     /locus_tag="ACHOQW_000442"
     CDS             29359..30237
                     /gene="dapA"
                     /locus_tag="ACHOQW_000442"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9571574.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30254..31288
                     /gene="bamC"
                     /locus_tag="ACHOQW_000443"
     CDS             30254..31288
                     /gene="bamC"
                     /locus_tag="ACHOQW_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP9571575.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31501..32214
                     /gene="purC"
                     /locus_tag="ACHOQW_000444"
     CDS             31501..32214
                     /gene="purC"
                     /locus_tag="ACHOQW_000444"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9571576.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32382..33245
                     /gene="ypfJ"
                     /locus_tag="ACHOQW_000445"
     CDS             32382..33245
                     /gene="ypfJ"
                     /locus_tag="ACHOQW_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP9571577.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33260..35275
                     /gene="tmcA"
                     /locus_tag="ACHOQW_000446"
     CDS             33260..35275
                     /gene="tmcA"
                     /locus_tag="ACHOQW_000446"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP9571578.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35349..36047
                     /gene="ypfH"
                     /locus_tag="ACHOQW_000447"
     CDS             35349..36047
                     /gene="ypfH"
                     /locus_tag="ACHOQW_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9571579.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36128..36328)
                     /gene="ypfN"
                     /locus_tag="ACHOQW_000448"
     CDS             complement(36128..36328)
                     /gene="ypfN"
                     /locus_tag="ACHOQW_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP9571580.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36356..37483)
                     /gene="dapE"
                     /locus_tag="ACHOQW_000449"
     CDS             complement(36356..37483)
                     /gene="dapE"
                     /locus_tag="ACHOQW_000449"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP9571581.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37487..37843)
                     /gene="yffB"
                     /locus_tag="ACHOQW_000450"
     CDS             complement(37487..37843)
                     /gene="yffB"
                     /locus_tag="ACHOQW_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP9571582.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
     gene            38224..38283
                     /gene="ypfM"
                     /locus_tag="ACHOQW_000451"
     CDS             38224..38283
                     /gene="ypfM"
                     /locus_tag="ACHOQW_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP9571583.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(38382..41495)
                     /gene="acrD"
                     /locus_tag="ACHOQW_000452"
     CDS             complement(38382..41495)
                     /gene="acrD"
                     /locus_tag="ACHOQW_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP9571584.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(41659..43359)
                     /gene="narQ"
                     /locus_tag="ACHOQW_000453"
     CDS             complement(41659..43359)
                     /gene="narQ"
                     /locus_tag="ACHOQW_000453"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP9571585.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43565..45544
                     /gene="aegA"
                     /locus_tag="ACHOQW_000454"
     CDS             43565..45544
                     /gene="aegA"
                     /locus_tag="ACHOQW_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP9571586.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            45612..46187
                     /gene="nudK"
                     /locus_tag="ACHOQW_000455"
     CDS             45612..46187
                     /gene="nudK"
                     /locus_tag="ACHOQW_000455"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP9571587.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            46313..47356
                     /gene="ypfG"
                     /locus_tag="ACHOQW_000456"
     CDS             46313..47356
                     /gene="ypfG"
                     /locus_tag="ACHOQW_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP9571588.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(47451..49454)
                     /gene="tkt"
                     /locus_tag="ACHOQW_000457"
     CDS             complement(47451..49454)
                     /gene="tkt"
                     /locus_tag="ACHOQW_000457"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9571589.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(49474..50424)
                     /gene="tal"
                     /locus_tag="ACHOQW_000458"
     CDS             complement(49474..50424)
                     /gene="tal"
                     /locus_tag="ACHOQW_000458"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9571590.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            50713..52992
                     /gene="maeB"
                     /locus_tag="ACHOQW_000459"
     CDS             50713..52992
                     /gene="maeB"
                     /locus_tag="ACHOQW_000459"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP9571591.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            53285..53620
                     /gene="eutS"
                     /locus_tag="ACHOQW_000460"
     CDS             53285..53620
                     /gene="eutS"
                     /locus_tag="ACHOQW_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP9571592.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            53633..54112
                     /gene="eutP"
                     /locus_tag="ACHOQW_000461"
     CDS             53633..54112
                     /gene="eutP"
                     /locus_tag="ACHOQW_000461"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP9571593.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            54087..54788
                     /gene="eutQ"
                     /locus_tag="ACHOQW_000462"
     CDS             54087..54788
                     /gene="eutQ"
                     /locus_tag="ACHOQW_000462"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP9571594.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            54785..55516
                     /gene="eutT"
                     /locus_tag="ACHOQW_000463"
     CDS             54785..55516
                     /gene="eutT"
                     /locus_tag="ACHOQW_000463"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP9571595.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            55513..56529
                     /gene="pta"
                     /locus_tag="ACHOQW_000464"
     CDS             55513..56529
                     /gene="pta"
                     /locus_tag="ACHOQW_000464"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9571596.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            56568..56861
                     /gene="eutM"
                     /locus_tag="ACHOQW_000465"
     CDS             56568..56861
                     /gene="eutM"
                     /locus_tag="ACHOQW_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP9571597.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            56968..57255
                     /gene="eutN"
                     /locus_tag="ACHOQW_000466"
     CDS             56968..57255
                     /gene="eutN"
                     /locus_tag="ACHOQW_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP9571598.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            57267..>58527
                     /locus_tag="ACHOQW_000467"
     CDS             57267..>58527
                     /locus_tag="ACHOQW_000467"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP9571599.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNG"
BASE COUNT        14162 a        15185 c        15579 g        13601 t
ORIGIN      
        1 tatcaattgg aactttcata tgacacttag aatcaatgct ctctatagtg atgaataatc
       61 atcattcgaa gtcaggtggg atgcctgtct gaatacacct ccttcaggat gtgggggatt
      121 ctgctgagca tctatgaaac tgaatgcaac ttatataaaa atacgtgata aatggtgggg
      181 gcttccgctg ttcctgcctt ctttaatctt gcccattttc gcccacatta atactttcgc
      241 gcatatttct tccggtgagg tttttctctt ttatctgcct ctggcactga tgatcagcat
      301 gatgatgttt ttcagctggg cggcattgcc agggatcgcc ttagggattt ttgtccgcaa
      361 atatgcagag ctgggttttt acgaaacgct ctcattaacg gctaatttta ttatcattat
      421 cattctctgt tggggcggtt acagggtctt tactccccgg cgtaacaacg tttcacatgg
      481 tgatacccgt ttaatttccc agcgtatatt ctggcagatt gtgtttcctg caacgctgtt
      541 tctgatactt ttccagtttg ctgcatttgt aggattactg gcgagcagag aaaatctggt
      601 tggcgtcatg ccttttaatc tcgggacctt aatcaattat caggccttac tggtgggcaa
      661 tctgattggt gtcccgctgt gctacttcat cattcgggta gtgcgaaatc cgttttattt
      721 acgtagttat tattcgcaat taaaacagca ggttgatgtc aaagtcacca aaaaagagtt
      781 cgcactctgg ctgctggcat taggtgcttt actgttgctg ttatgcatgc cgttaaatga
      841 aaaaagcaca atttttagca ccaattatac cttgtcatta ttgctgcccc tgatgatgtg
      901 gggagcgatg cgctatggtt ataagctgat ttcgctgctc tgggcggtcg tgttgatgat
      961 cagcatccac agctatcaaa attacattcc catttatcct ggctatacca cgcagctgac
     1021 cataacctcc tccagttatc tggtattctc ttttattgtc aattatatgg ctgtactggc
     1081 aacccgtcag agaacggtag tcagacgcat tcagcgcctt gcgtatgtgg acccggtggt
     1141 tcatctgcca aatgttcgcg ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct
     1201 ttgttattta cgcatccctg gcatggaaat gctggttaaa aactatggca tcatgctgcg
     1261 gattcaatac aagcaaaaac tttctcactg gctgtcaccc ttgctggaac caggtgaaga
     1321 tgtttatcag ctttcgggta acgatctcgc gctgcgactg aatacagaat cgcaccagga
     1381 gcgcattacc gcactggata gccatctcaa gcaatttcgt ttcttttggg atggcatgcc
     1441 gatgcaaccg cagattggcg tcagttactg ctatgtgcgc tcgccagtga atcatatcta
     1501 cctgctgctg ggagagctaa atacggtcgc cgaactttcc atcgtgacca acgccccgga
     1561 aaatatgcag cgtcgcgggg caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat
     1621 gatgaatcga ctacagcagg cgctggaaca caaccatttt ttcctgatgg cccagccgat
     1681 taccggtatg cgtggtgatg tttaccatga aattcttctg cgcatgaaag gtgagaatga
     1741 tgaactaatc ggccccgata gcttcttgcc ggtcgcgcac gaatttggtt tatcgtcgag
     1801 tatcgacatg tgggtcattg agcatacgct gcaatttatg gctgaaaaca gagcgaagat
     1861 gcccgctcac cgttttgcta ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc
     1921 tgttgaagtc agtcagttgc tggataaata tcagattgaa gcgtggcaac ttatttttga
     1981 agtcaccgaa agtaatgctc tgaccaatgt taagcaggcg caaatcacct tgcagcatct
     2041 tcaggaatta ggctgccaga ttgcgattga tgatttcggc accggctacg ccagctatgc
     2101 gcggcttaaa aatgtgaatg ccgatctgct taaaattgac ggcagtttta tccgcaatat
     2161 tgtgtcaaat agtctggatt atcagatagt ggcatcgatt tgccacctgg cgcgaatgaa
     2221 gaaaatgcgg gtagtggcag agtacgttga aaacgaagag atccgcgagg cggtgctctc
     2281 tttggggatc gattatatgc agggttatct tattggtaag ccgcaaccgt taattgatac
     2341 gctgaatgaa atcgaaccca ttcgcgaaag tgcctgaata atgcgggccg acatttctcg
     2401 tcggcccgca aagcattaag ctgcgatttc aggtgtactt tcttcttcaa ttttcaaccg
     2461 ccagccagcc acgccttccc agtattcttg ctccttttcc agatcaagca gtaccagcgc
     2521 attctgacta aaccagtcat gcgggaaacg cagtgtccag tgactgtcat cagtaatcag
     2581 cgtcaatgtt ggcggtgtgg tggttgcctg acgctgattg ttgagtaata cgccaaggcg
     2641 caatagctga atcagtggca ggaactgttt cttcttaaac aaggtaaagc gcggcagatc
     2701 gtcgagttta atcgctttac ggtgatagcg caccagtgtc gccatcatca gctgctgttc
     2761 ctgattaaaa cccggcaagt cgctgttttg tagaatataa gcggagtgac gatgcaaacc
     2821 gctgtggttg atattcaacc cgacctcatg cagcatggcg gcccatcgca gtagcgcctc
     2881 cagttgcgga tgcgccagct tcggttgctg ttcccgccac tgttcgtaca tttgcatagt
     2941 ggtatccagc acccgtcggg cctgttcgct gtcgatgtga tactggttgg cgaggctgct
     3001 ggcggtgcga ctacgcacat cctgatgacg gaaacgtcct tccatttcat acagtacgcc
     3061 ttcgcgtaac gccccgtcag aaaggcgcag ttcacggatg gctaaagcat caaacacacc
     3121 gcataaaatc gctagtccag gaacgaagac tgttttccgc tcttcggaaa gacctggtaa
     3181 actcagcgat gcgaaattac ggtgccgtaa aacttctttt accagttttt ccagacgttc
     3241 cggggtaatt atcccgtctt tctcgcccat ttccattaac acttcatggg cggcttttat
     3301 ggtaccggaa gcgcccattg ccacgttcca gccctgaata cgaaattgcc aggttaaagt
     3361 ttccagtttt tgtgctgccg ccatgcgagc gcgctgaaaa ttctctttat tgatgacccc
     3421 gccagggaaa taaagctggg caaagctgac acaccccatc cggcggcttt caacgagaat
     3481 cggttcgaaa ttttcaccaa tcaccagttc tgtagaaccg ccgccaatat caataaccag
     3541 tttgcgacct ttttccggtt gggtatgttc cacgcccata aaaatcagac gggcttcttc
     3601 attaccggaa ataatttcaa tcgggtaggg aatgaccttt tccgcacgtt tcagaaagtc
     3661 agtggcgttc agcgcctgac gcagcgtatg ggtaccaact atacagacgc tggcaggaga
     3721 aaacccttgt agccgttcgg caaacagcga cagacagttc aaaccgcgcg tcattgcctc
     3781 ttcactcaac atattatctg gccccaggcc gtccgccaga tgcacccgct gtttcaggcg
     3841 gccaataatc tgcatggcac catctaccac acgggctatg accatgtgaa aactgtttga
     3901 accaagatcg accgcagcaa actcctgcgg acgaggggat ttatcgtgta ttggcatagg
     3961 gttattcagg ttgttcgagt gatttgatgt agtcataaat cgccaactgc gcccgtactt
     4021 tgcggcgatt gccgcgggga acgtagcgat tactgagttc tttatcgata taacgtgctt
     4081 tgaccgtatc gctgaacaat atgtcgatga tgtccagcac ccgctgcttc aggcgcggat
     4141 cgagcagcgg cgtcgccact tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg
     4201 aagaaaggta gaccttttta tcgccgccat tttcaaaaat ataaacccgg tcatgttcaa
     4261 ggtaacggtc aacaatactg atggcacgaa tgttgtcgct aatgccttcc agattgggga
     4321 tcagcgaaca cattccgcga accagcagat taaccggtac gccggagctg gaggccgcat
     4381 acagacgatc aaccaggcct ttatcgacaa ggttatttag cttcagggtg ataccactgg
     4441 gcagcccttg ctgcgcgttg gcgatctcgc ggtccaccat ttcatacaac aggcggcggg
     4501 agttttgcgg cgacaccatt aaataatcaa atgtcaccgg acggtatggg ttttcaataa
     4561 agttaaatac ccgccgtact tcgttggtga tgcgcgcatc ggcggtcagc aacgaatagt
     4621 cagtataaag acgcgcggtt ttttcgttaa agttcccggt cccgatgtgt gcataacgca
     4681 ccacttcacc gttttcttta cgtgaaatca gaaacagttt ggcgtgaatt ttcagccccg
     4741 gcgcagagaa gataacgtgc acgcctgctt cggtcaggcg cttcgcccag tgaatgttgg
     4801 cttcttcgtc aaaacgtgcc tgtagctcaa ccaccacggt gactttctta ccgttgtgtg
     4861 cggcgtggat catcgagtcg atgatgcgtg aatcttttgc cacacggtaa atgttgattt
     4921 taatcgccag cacgctcgga tcgaacgagg cctgacgcag cagttccagc acatgctcaa
     4981 aggtgtgata aggataatag agcaacacat cgcgttcgcg aatggcatca aaaccattgc
     5041 ggaactgggc tttatcaaac caaatatggc gtaaacgcgg cagtggtttg ttcaccagat
     5101 tggctttgcc gacattgggg aaattaataa agtctttaaa attatgataa cgaccgccgg
     5161 ggacgatgga gtcgtagcgg gaaatagtca gtttttcgcg taacacttca accagcgcat
     5221 tgggcatatc gcgctgataa acaaaacgca ccggctcagc agttaaacgc tgcttgagac
     5281 tggaagacat caactccatc aggctggctt ccatctcatg cactaaatcg tattcggcat
     5341 cgcgggtcat cttcattgaa taggcattca gcgcgtcata atcaaagaag cctttgaaaa
     5401 tatcatcaag gcagtaacgc agaatgttat ccagaagaat cattggcttg cgtcgacgcg
     5461 gcgcttctgg cggtaaattc acaaagcgcg gcactttatc tgatgggatc tccagaagcg
     5521 cgtaacggat ggtatcgcca cggataattt ccaccgccag ataggtgtaa tcatctttca
     5581 ggaactgcac taagtcagtg tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat
     5641 actgcttaaa ataatgacgc agccagtttt gttgattgac ggagagctgg cgttcattaa
     5701 tcaggaagat ctggttgcgc gccatctcca gcaatagctc gttgtagagg ccgtcgaatt
     5761 cctgatcggc tttcagcacc cgggactgaa ttttgcccag taaatggcgg gaatgagagt
     5821 tggagccttg ttcttcgcta ataatgatgc gtcgcttcag ttcagcgaag cggactttat
     5881 agaactcatc aaggttattg gaatagatcc ccaggaaacg catcctttca atcagcgggt
     5941 tagatttgtc cgccgcttcc tgaagcacgc gttcattgaa cgataaccaa ctgagctctt
     6001 tttcgatgta tagcttttcc tgacccatta ccacttttac tccgttttat tcacgggaca
     6061 ctgcctggat attatggcga gcaatattgg cagatgtcca actatgccag aaaaggggga
     6121 aaaaaactgc tggagcttgc gatttacagg tgaattgaaa aacgccagcg gcagagctgg
     6181 cgctttaact acggggcatt actcgtcggc agcgtagccc tgcggcggca gacgttgacc
     6241 atccagccac gcggcgtttt cgtgcatttt cagacgacca tcggcaaacc agctaatcac
     6301 cagtggataa atggcgtgtt cctgggtttg cacgcgggcg gtgacgtcat cctccgtatc
     6361 accagcaaat accgggactt tcgcctgtaa aataaccggg ccaccgtcca gttcatcggt
     6421 gacgaaatgc accgatgtac cgtgctcttc atcgccattt tccagcgcct gacgatgggg
     6481 atgtaatccg ggatatttcg gcagcagaga agggtgaatg ttcagcaaac gcccggcata
     6541 gtgggagaca aatgccgggc tgagaatgcg cataaaacca gccagcacga ccacatcggg
     6601 tgcgtacatg tcgatttcat gaatcaactc ccggtcatag gcttcacgac tgtcaaacgc
     6661 gctggcgatg agcgtatgcg ttgcaatacc cgcctggcgg gcgcgttcaa ggccgaacgc
     6721 gtcggcctta ttgctgaaaa ctgcccgtac ggtgccttta attttgttgg ttttacaggc
     6781 gtcaataatt gcctgcaaat tacttccgtt gccggaaata agcaccacaa tattcattat
     6841 tcgataacca cgcgttgttc ggaatcagag gctttgatga taccgatttt ccacgcgttt
     6901 tcaccgttgg cattgagcag ggcgagggct ttgtccactt ccggagcagg cagggcaata
     6961 atcatcccga cgccgcagtt gaaggtgcga tacatttcat ggcgctcaac gttaccagcc
     7021 gtttgcagcc agttgaacac ttccggccac tgccaggagg attcatcaat cactgcctgg
     7081 gtattatctg gcaatacgcg cggaatgttt tcccaaaagc cgccgccggt caggtgcgca
     7141 atggcattaa catcgacctt ttcaatcaac tccagcactg acttcacgta aatgcgggtc
     7201 ggtgccagca gatgatcggc taatggctta ccatcaagtt cggtggtttg cggatcacaa
     7261 ccgctgactt caagaatttt gcgcaccagc gaatagccgt tcgagtgcgg accgctggaa
     7321 ccgagtgcaa tcagcacatc gccgtcgctg actttagagc cgtcgatgat ttctgatttt
     7381 tctaccacgc ccacgcagaa acccgcgaca tcgtaatcct caccgtgata catccccggc
     7441 atttctgccg tttcgccacc caccagtgaa cagcccgatt gcagacaacc ttccgcaatg
     7501 ccgctgatca ccgctgaagc ggtatcaaca tccagttttc cggttgcgta atagtcgagg
     7561 aaaaacagcg gttctgcacc ttgcaccacc aggtcattaa cgcacatggc gaccagatca
     7621 ataccaatgg tgtcgtgacg ttttaagtcc attgccagac gcagcttggt acctacgccg
     7681 tcagtgccgg aaaccagcac gggttcacga tatttttgcg gcaatgcaca cagcgcaccg
     7741 aagccgccca gaccgcccat cacttccgga cgacgcgttt tcttcactac gcctttgatt
     7801 cttccaacca gagcattacc cgcgtcaata tcaacaccgg catctttgta gctaagagag
     7861 gttttatcgg tcactgcttg ggtccccacg cgttacttgc ggtagaaaaa taaaattcgg
     7921 cgcaattcta acagggaaag caaacgtttg cgagactgct ttacacaacc tttttgcacg
     7981 tcttttcccc aggcgcgcgg cgaaagaaga cttgtgccag ggcaaaggtt agttttcgga
     8041 tggaataatc ttctttcata accatttgaa tataaaataa ctttatctca aaccgttatc
     8101 attttgacta aagtcaacga aaataatatt gccgccttga agaaaggagg tataatccgt
     8161 cgattttttt tgtggctgcc cctcaaagga gaaagagtat gaagatcgtg gaagtcaaac
     8221 acccactcgt caaacacaag ctgggactga tgcgtgagca agatatcagc accaagcgct
     8281 ttcgcgaact cgcttccgaa gtgggtagcc tgctgactta cgaagcgacc gccgacctcg
     8341 aaacggaaaa agtaactatc gaaggctgga acggcccggt agaaatcgac cagatcaaag
     8401 gtaagaaaat taccgttgtg ccaattctgc gtgcgggtct ggggatgatg gacggtgtgc
     8461 tggaaaacgt tccgagcgcg cgcatcagcg ttgtcggtat gtaccgtaat gaagaaacgc
     8521 tggagccggt accgtacttc cagaaactgg tttctaacat cgatgagcgt atggcgctga
     8581 tcgttgaccc aatgctggca accggtggtt ccgttattgc gaccatcgac ctgctgaaaa
     8641 aagcgggctg cagcagcatc aaagttctgg tgctggtagc tgcgccagaa ggtatcgctg
     8701 cgctggaaaa agcgcacccg gacgtcgaac tgtataccgc atcgattgat cagggactga
     8761 acgagcacgg atacattatt ccgggcctcg gcgatgccgg tgacaaaatc tttggtacga
     8821 aataaagaat aaaaataatt aaagccgact ttaagagtcg gctttttttt gagtaaagcg
     8881 cctataacac ataatacaga ggataatact atgacgcgcc gtgctatcgg ggtgagtgaa
     8941 agaccgccac ttttacagac aatcccgctt agtttgcaac atttgttcgc catgtttggt
     9001 gcaaccgtcc tggtgcccgt cttatttcat attaacccgg cgactgtact gttatttaac
     9061 ggtattggaa cgctgctgta tctcttcatc tgtaaaggga aaattccggc ttatcttggt
     9121 tccagctttg cctttatttc accggtattg ttactgttgc cgttagggta tgaagtcgcg
     9181 ctgggcggct ttattatgtg cggcgtgctg ttctgcctgg tttcttttat cgtgaagaaa
     9241 gcggggaccg gctggctgga cgtgctgttt ccacctgcgg caatgggcgc aatcgttgcc
     9301 gtcatcggtc tggagctggc gggcgtagct gccggtatgg cgggtttact cccggctgaa
     9361 gggcaaacgc cagactccaa aaccatcatc atctctatta ccaccctggc ggtcacggtt
     9421 ttaggttccg tgctgttccg tggtttcctg gcaattatcc cgattttaat tggcgtgctg
     9481 gtggggtacg cgctctcttt cgcaatggga attgtcgata ccacgccgat tattaatgct
     9541 cactggtttg cgctgccaac cctctatacg ccgcgcttcg agtggtttgc cattctgact
     9601 attctgccag cggcgttagt ggttattgcc gaacacgtag ggcacctggt agtaacggct
     9661 aatatcgtca aaaaagatct gctgcgcgat ccaggtctgc accgttcgat gtttgctaac
     9721 ggcttgtcga ccgtgatctc cggcttcttt ggctccacgc caaacaccac ctacggggaa
     9781 aacattggcg tgatggcgat cacccgtgtt tacagtacct gggttatcgg cggggcggcg
     9841 attttcgcta tcctgctttc ctgcgtcggt aaactggctg ccgctatcca gatgatccca
     9901 ttgccggtga tgggcggcgt ttcgctgctg ctttatggtg tcatcggtgc ttccggtatt
     9961 cgtgttttga tcgaatcgaa agtggattac aacaaagcac agaacctgat cctgacttcc
    10021 gtgattttga tcatcggcgt cagtggcgcg aaggtaaaca ttggcgcggc ggagctgaaa
    10081 ggtatggcgc tggcgaccat cgtcggtatc ggcttaagtt tgattttcaa actgattagt
    10141 gtgctgcgcc ctgaagaagt ggtgctggac gcagaagacg cggatataac agacaaataa
    10201 tgtaccgacc gggcagtgtt gctgcccggt tcaaacatca tgggattctg tggtaaactt
    10261 ctcgcgattt tgtgaaatcc tggttgaggt atctctgaac acaccggcac agctctcttt
    10321 gccactttat cttcctgacg acgaaacctt tgcaagtttc tggccggggg ataactcctc
    10381 tttactggcc gcgctgcaaa acgtgctgcg tcaggaacat agcggttaca tctatctctg
    10441 ggcacgcgaa ggcgcggggc gcagccatct gctgcacgcg gcttgcgcgg aattgtcgca
    10501 gcgtggcgat gcggtgggct atgtcccgct ggataaacgc acctggtttg ttccggaagt
    10561 gctcgacggt atggagcatt tgtcgctggt ctgtatcgac aacattgagt gtattgcagg
    10621 cgatgagttg tgggagatgg cgattttcga tctctacaat cgaattctgg aatcgggcaa
    10681 aacacggttg ttgatcaccg gcgatcgtcc accgcggcag ttgaatctgg gattaccgga
    10741 tctcgcgtcg cgactcgact gggggcagat ctacaaattg cagccacttt ctgatgaaga
    10801 taagttgcag gcgctacagt tacgcgcgcg tttgcgtggt tttgaactgc cggaagatgt
    10861 ggggcgtttc ttgctgaagc ggctcgacag agaaatgcgc acgctattta tgacgttgga
    10921 tcagttggat cgtgcgtcga ttaccgcgca acgtaagctg accattccgt ttgtgaaaga
    10981 aattctgaag ttgtagataa ggtgtttatt gtcggatgcg gcgcgaacgc cttatccgaa
    11041 ctacgcgaag ttaatccata gaattcgtag gccggataag acgtggcaag cgtcgcatcc
    11101 ggcaatgtgc ttcaactagc gatggagcac atcttaaccc acgatttcca gtacctgctc
    11161 cggtggacga ccaatccgcg ctttgccatt cgcgaccaca atcgggcgct ccatcagctt
    11221 cggattatcc accattgcct gaatcagcgc ctcttcgcta agcgaactgt cagccaggtt
    11281 cagttcttta taaagatcct ctttctggcg catcaattca cgggcgctat tcatccccag
    11341 cattttcagc aaatcgcgca gcgttgccgc atcggcgggt gtctcaaggt agagcaccac
    11401 ttctggctcc acgccgtttt ctttcagcag gttcagcgtt tcccggctct ttgaacagcg
    11461 tgggttatgg tagattttta cctgtttggt cattgttctt cctttaatgc gaattacatc
    11521 ttggtataag gcttaaagcg ttcctgcagc tggcgcaact ggtcgatgcg cgcatcgtaa
    11581 cgcgcttgtt gcaggctgcc taatttcacc tgcgaactgg cgctactcaa cagcgaaatg
    11641 gcctgatcga gtcgtccggc gagcgcataa ccttctgctc gcgcagccag ctcctgatcg
    11701 cggttattta gcgcggcttc cgcctgtgcc agcaaatccc agccgttgct gtcatcttta
    11761 ttattaaagg tgtagcgatt aagaatattg gccgcttctt gtggttgacc gccttgtaga
    11821 taagcgttcg ccaggttgag ctgcaacaca ggattggtgc gcaaatcgcg ggcatttttc
    11881 agacgattga tcgcctcatt ggctttgttt tgcccaagat cgatatcagt agccagatcg
    11941 agataccatg cgttgccagg ttctgccgcc agtaacggtt gcagcgtttt tcgcgcctcg
    12001 tcgtatttat tggcttccat cgcctgtaaa gcacgaccat attgcgccgc tcgttgctga
    12061 cgaacgtttc ctttcgccca ttcatccagc aaatcactgg tgagctggtt acgtccggaa
    12121 ttatacatcc ccagtgtgcg cgctttcgcc agatagaaat cttccgacga ctgcaccacc
    12181 atcgggcgca tctgattagc acggttgcgg gcatctgcca gacgactttc cggcaacggg
    12241 tgagtcagta aaatttccgg cgggcgcgag gagtaacgcg cctgatcgag taatttttcg
    12301 aggaaggttg gcatcgcctg cggatcgaat cccgagcgtt gcagcacctg aataccaatg
    12361 cggtccgctt cctgttcatt ttgctgggtg aaactgatca tcccctgacg cgttcccgcc
    12421 agtgtaccgg tcagcgccgc catccccgcc tgcggactgg ccatcgccag taaaatagaa
    12481 cctaacgcgc cgacccaggt cagcggcgcg ttgcgctgct gatcttccat cgctcgcgcc
    12541 aggtgacgtt gggtgacgtg ggagatttcg tgcgccataa ctgaagccag ttgactttcg
    12601 ttatcggaat aacggaacag ggcagagtgc agcaccacgt tgccgccaaa gaaagcaaag
    12661 gcgttaattt cgtcgttgtt gatcagaaag aaatgaaacg gtgtcttaac cgaattggca
    12721 tgcgaaacca gacgcatccc cagcgaatta atatattgcg ttaacagcgg gtcattaatt
    12781 aacggcgcgc tgccgcgtag ctggcggaca taatagtcgc ccatctgcat ttcctgacca
    12841 atggaaagcg tgcttcctgc ggaggttccc atatccggca aggtgtctgc gctgtcggca
    12901 aatgccgggg ctacctgacc aatggtcata gcagcaatga gggttgcaac caggtttttt
    12961 ttcaactgcc tgaacataac ctctatcctg tatttctgaa gatagtcatt tgaccgatac
    13021 tgtgaaatat cgttcccacc gcgccgcctt tgagtgtaac ggcgaagatt agcccactca
    13081 aatgactttt cactttcctg aatccgtcat aaaaagcgaa gtcttttttg tgacatttcg
    13141 atacaattgc ggatcgcaat cccgctcttg aggttcaggg aaggtcttta tgctcgaaat
    13201 gttgatgcaa tggtatcgcc gccgttttag cgacccggaa gcgattgcct tgctggttat
    13261 tttagttgcc ggatttggca ttatcttttt ctttagtggc ctgcttgctc cgttgctggt
    13321 ggctattgtg ctggcctatt tgctggaatg gccaaccgtg cgcctgcaat ctattggctg
    13381 ctcccgccgc tgggcgacgt cgattgtatt ggtggttttc gtcggtatat tgctactgat
    13441 ggcgttcgtg gtactgccta tcgcctggca gcagggcatc tacttaatcc gcgatatgcc
    13501 ggggatgctc aataagcttt ctgactttgc cgccacgttg ccgcgccgct atccggcgtt
    13561 aatggatgcg ggcattattg atgcaatggc cgaaaatatg cgcagtcgga tgctgaccat
    13621 gggcgattcg gtggtgaaaa tttccctcgc ctcgctggtc ggtttgctga ccattgccgt
    13681 ctatctggtg ctggtaccat tgatggtctt cttcctgctg aaagacaaag agcagatgct
    13741 gaatgccgtt cgccgggtgc tgccgcgtaa ccgtggactg gcaggacagg tgtggaagga
    13801 gatgaatcaa caaatcacca actatatccg cggcaaagtg ctggagatga tcgtagtagg
    13861 gatcgccacc tggctgggat tcttactctt cgggctgaac tattcgctgc tgctggcggt
    13921 gctggtcggc ttctcggtac ttattccgta cattggcgca tttgtggtga ccattccggt
    13981 ggttggcgtg gcgctattcc agtttggtgc aggcactgaa ttctggagct gtttcgcggt
    14041 gtatctgatc attcaggcgc ttgacggcaa cctgttagta ccggtgttgt tctccgaagc
    14101 ggttaacctg catccgctgg tgattatttt atcggtggtg atcttcggtg gtttgtgggg
    14161 attctggggc gtattcttcg ccattccatt ggcgacgctg atcaaagccg tgatccacgc
    14221 ctggcccgat gggcaaatag cgcaagaata atcttcagcc cgacgaactg tttcgccggg
    14281 ctgatttttc aggattcctg acgtaaataa atagcccgat aacaaatact caccagcact
    14341 gcaccgccga taatattacc cagcatcacc gggagcagat tggcggtaat aaaatggctg
    14401 accgtcagtg caggaaaatt gtctgcgcta ctgtgcgcca gttgccagaa ggaggtaggg
    14461 gcgaaatggc gaatggcaat ggcgaacgga atcacaaaca aattggcgat acagtgctca
    14521 aagccactgg cgacaaacag ggtgatgggc aaaatcatgg cgacgatttt gtcgcataac
    14581 gaacgcccgc aataactcat ccacagcgcc aggcaaacca tcagattgca cataatgccg
    14641 aggctgacag attcagtaaa tgtatgatgc attttgccct cggcgcagtg taaaaccgcg
    14701 actccccaca tcgcgttttc actcatcacc agcccggaaa accagattaa caaactgaaa
    14761 caggcaatac ctgccagatt accgcaggcc accagaagtg cgttaatcag ccaagttcgc
    14821 caactaataa cgccccgact ttttgccatc accgtcatta ccgacgaggt gaacaggctg
    14881 gtgccgcaaa ccgccagcaa aataaagccg agtgtaaagc aaaggccgcc caccagatgg
    14941 gttaatgcct gtgaagacgg ggcatcggcg ataacggaaa ggtaaaaagt aaatccgatc
    15001 gccataaaca cgccagcgga catcgccatt aaaaagaccg acactttact gcgggcaatt
    15061 ttatgggcgg caatccgttc agcgattgcc gcttttctgg cgctcaactg caagtcgaaa
    15121 gagagtttgt ttctcatgca tactcctgca aaagcagatt acaacacctc gcgaaccgag
    15181 atccccagac gctgcattcg tgacagcagc gtggtgcgct tcatccctaa tcgtgtcgcc
    15241 gcgccacggg gcccggcaac aatgccattg gtttctcgca atacctgaac aatgcgctga
    15301 cgttcttctt cgtcattctc tggcgtcgtc gggtgcagca actgcgccat tgaactgcga
    15361 agcgctgaat cttcatttag cgtcggcagt aaacggcttt gtcggacatt aagatgtaaa
    15421 ttcagactgt taccgcgagt cagcagtacc gctcgctcaa tcacgttttc cagctcgcgc
    15481 acgttgcccg gccaatccca cgacatcaac tggcgtagtg cctcggtcgg gatggcgtca
    15541 attgcgcgat tcatatggcg cgccattttt tgcgtgaaat gttttgctaa aagagggata
    15601 tcttccggac ggtctcgcag cggcggcaat tccagtggga agacattcag gcgataaaag
    15661 agatcgctgc gaaactggcg atcttcaacc atttgccaca aatcacggtt ggtggcggca
    15721 atgactctga cattcaccgg gatcgttcta ctcccgccga gacgctcaat ttcccgctcc
    15781 tgcaatacgc gcagcaattt aggctgaagt tctaacggca gatcgccaat ttcatcgaga
    15841 aacaacgtac cgccatcggc aatttcaaaa cgaccacgat gggtattaat cgcaccagta
    15901 aaggcccctt tgtcatgacc gaataactca ctttccagaa gactggcggg gatggcagcg
    15961 cagttgattt tgaccagcgg cttgtcgcgt cgcgggctaa gttgatggat cgctctggcg
    16021 atcacctctt tgccggttcc ggtttcacca caaatcaaca ccgtactgtc gctcttcgcc
    16081 acaatatcta cctgctggag caggtcttcc attgcctggc tttgatagat aatgtcaccg
    16141 acgcccagat tcgaaaggag ctgctcgcta agctggtggt tttcttgctg caaactttcc
    16201 tgcaaatcgg tcatgctacg ccaggcatcg gcattgccaa cggcaatagc gatgcgatcg
    16261 gctatgtgtt gtagtagctg gcagttttcc tcactaaaga gagtggtaga ggtatgcgcc
    16321 agcaacaatg cacccggtgt atggttgcca aaggtaagcg gtatcaggag cgaagattcg
    16381 cagccgttag atgcaagtaa gtgtaataac gggtcgcgtt gccagagaaa cagatcgtct
    16441 gcacggtgcg tcaaggtcgg ttgtcgggtt tgtagcgttt gtgtcagcaa tacactgtcg
    16501 ccaggcatat tgcgtggcag acacgcacaa tgtgaggcag aaagatcgct gcaccacagg
    16561 ctgaacttct cgttctttcg atgatcgccc agtaccatac tgactgaagc cagaccgaaa
    16621 aaatgatgga tctcacgagc gacgtcagcg atcagatcgt cgagatcaag atgtgacagc
    16681 actgcattag tgatatccac cagtacccga tgatgatcgc gatctttctc cagcaaacgc
    16741 agcgccacct gttgttcttc atcattgagc catgattgca gaaccggggt gataatgcgt
    16801 gccagccagt taaacagtgt gcaatcctgg ggagtaaaac gactgccgtg ggtgcgaata
    16861 aagcgtagct ccgccattcc actactgccg agaaggacgg cgtgacgcga ataatcgacc
    16921 atctcgccct gatactctgt tgttgccgtc acatcgctgc catcatcatg gtagcggtaa
    16981 tagaagttgc ttttgagaac atgcagttca ataagttgta ccgggctgaa cggcggcggc
    17041 agcgggatga aggcgcgtaa taaagacgcc ttgccgtgtt tctgcgctaa ccgctctgcg
    17101 agcattccgt ttaccgcttc aattgttatt ccttgcggcg gggcaaacat cgcctcgtct
    17161 gacatagcca tcaacagtcc ttattaatga gaaatccttc tcacgcaatt tttctggcca
    17221 tcagatagat agccggttcc agcacaattt catccaacag atgaatgaat tgaatgctac
    17281 gcgcttgcca cacagacgtc gcgtcatctg ccagtggcgc gaatgcccgg gtcaacaggg
    17341 cggtgtggct ggcgtcaata acaatctcac caaaagtcat cacccccagc atcttacgtc
    17401 gggcttgctc ctcttcgacc agtgcaagcc aatcttcgta ttccgtcagt gggcagcgga
    17461 aggtgacatt cagacaatca atcacgccaa cgtgatgacc gatagccagc gagtaataca
    17521 ttacctggga gctgtgttcc ggcatctcgt cgctactggc gacaaacttt tttcgcagcg
    17581 tccagaaaac aatttccccg cactcttcag tcatgtaacc cccggagtac gcgaaagagt
    17641 tgctgaacga tactattgag acgtggatcg tcggcatcgc gcaaccaggt gttaacccga
    17701 tttcctgtag gatcgttgct taaatggcgc aggagccgat cgcaaatttc ttgcccctga
    17761 cgatagcccg ccagccgccg cgcttctcgc tcaatggcga tacgctgtga tggcgggatc
    17821 tgcggccaca acggttgcat agtcaccccg gtgggatcgc gataatccac cgcgtgaatc
    17881 ttctgttgca gcaaaccgag cgccacggcg aaaccgtgaa tggtggcggc cggtgttggc
    17941 gggcagccgg ggatccaaac atcaatgggg acaatggtgt cgctgccgcc ccagacgctg
    18001 tagagatcgt ggaaaatacc gccgccgaca ccgcacgcgc cgtaggaaac acagatttta
    18061 tgatcggggg cagactcata cgcccgaagt gcaggcatac gcatcgcccg ggtgactgcg
    18121 ccagtaaata acaaaatatc ggcgtgacgc ggtgatgaaa caaccttaat gccaaaacgt
    18181 tctgcgtcga atacgggtgt gatggcggca aaaatttcga tttcacaggc gttgcagccg
    18241 ccgcaatcga cgcgataaac gtaggctgag cgacggatat cctgtagcaa atgccgcttc
    18301 atcttttgcg tttgctcgtc cagcgtgatg ggctggctga catgttgtgt aagcactgga
    18361 ctcatagctg ctccttagcc accagcggta catctgtatc gtcgttgatc agcgtcgcgc
    18421 gttgtttgca ctccgggcag acgctcgctt gcgcccgcaa catttcgcgg ttttgtggcg
    18481 cattttgttg ctgtgccaac aattcagcag ccagtgcgac ggttttttgc ggggcaaacg
    18541 ggcgttcgca acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag agatcggctt
    18601 tattggtgac ggtcagttca aagttattgg taagctggat ggctctggtc gggcacactt
    18661 cttcacaacg tccgcagtaa atacaacgcc ccagatagag ctgccaggtg cgcgaatttt
    18721 gctggtcgtc ggtctggata gtcagcgcat ttgtcggaca agcacaggcg caggcaccac
    18781 aggcaataca ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca
    18841 gtggcgcgaa gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact
    18901 tcagcatctt ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga
    18961 tctctttata cggcacggtt tttgactggc gcttgcgcac atcaaccagc gtcacacggt
    19021 cggtacagga gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat
    19081 tgccgcgcag catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc
    19141 agcggaacaa tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg
    19201 cttcaacaaa gccgagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg
    19261 gggtatccgg catgttgtcg agagcaaatt ccagcattgc cagcgaatca aacgtctctt
    19321 tgacacggac catcacgcgg gagaaaacat cgccgccggt gaaggtaaac agtgtttttg
    19381 gaatattgcc gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc
    19441 cgcggatcag cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa
    19501 tgccctgagt gcgttgttcc atattcggcg tggcaagcag catttctacc agctccgaca
    19561 cgtcggcgcg catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat
    19621 cgcggcgaac accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt
    19681 ccgccattgt catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc
    19741 caacaaaatg gcaggagagg ccaaggttga gcaaatgact gtgtagccgt tcgacttcca
    19801 gcagaatcga gcgaatggta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa
    19861 ccgagttggt ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cggtccgata
    19921 agaaggtcac ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga
    19981 cataaaacag acggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt
    20041 gacccggttc atcggaggtg atatgcagcg ggccgacagg gatcacccgc gcaccgctgt
    20101 tgccctcatt gataaacgga tacgtttcgg aatcagtcgt cggttcaggg cgcagtcgat
    20161 aatccatcgc atctttgcgc agcggatgca tatcttccgg ccagtcatcg ggcaacacca
    20221 ggcgacgctg atccggcagg ccaaccggaa tcagcccgta catatcacga atttctcgct
    20281 cgccccagac cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat
    20341 cgaccagcgc cttcaccaca atccagcatt tttcggcccc ttccattgaa agggcataat
    20401 aaaccgcgta atgaccgtta agtgtccgct cgtcgttgcc aaacaggacc ggaagccagc
    20461 catcatgttg ataataaaga tactgtacaa cgtcaggcag cagattgatt ttcaccgtaa
    20521 tggtgacctg ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt
    20581 tcagggcggc gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc
    20641 tccgaaacag atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc
    20701 aggtcgtgcg tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt
    20761 tgtggaatat gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg
    20821 gtcaaccagc cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc
    20881 gcagccatcc gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata
    20941 atcagcaggt gattacgtgc cagtccggcg gtaacggtca taaattcgct aagaaaaatg
    21001 ttgaagggcg gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat
    21061 ggcatgattt tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt
    21121 acattgccgg aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa ggtgtgcagc
    21181 agcgcggcaa aaattcccag cggccggcca atgcctagcg ccaccgcgac cagtcccatg
    21241 ttctccacgc tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat
    21301 gccgccacgg caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg
    21361 ccgatggcct ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc
    21421 aatacggcgg agagtagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc
    21481 caggcgtgca tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag
    21541 gccaacagca ttaatgttgg atcaagcaag gacgattgct taagaacctc gctccagaat
    21601 atcgccattt ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg
    21661 aacagaccaa aagcgacacc aacagtacaa ataatgatgt acttccatgc tgcttccagc
    21721 gatgaacgct gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc
    21781 gccgcccaca tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac
    21841 agatggaaga aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgctcaact
    21901 tcatgacgca tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg
    21961 gcgaggaaca aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct
    22021 cctgcatcag cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct
    22081 aagctatgta atactgtcac cgtgcgagtc gcagagtgcc ctcgtttccg gcaggcaaaa
    22141 cagagcagcg aaaaaagcag cggagtgagc aggagtaaag cgaacatcac agaataactc
    22201 atctcattat cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca
    22261 tatttttctt gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc
    22321 aatttccacc agttccggtg cacgccaggc tagaagcgcc agcaccagat gggagccgtt
    22381 ttccatcagg cagtaaccaa aaatttgccg caggatattg cgctggctaa caatgcacag
    22441 caggccaagc agaaaatgac ctaacgccac cgccagcgct ggtttcagcc cggtagccat
    22501 cggtagcttc acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag
    22561 tgccatcatt gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc
    22621 agcgtaagtc atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca
    22681 gatcagcagt tgctctgcgg cgaacaggca cgagagggtg gcgaaaatag acaccagcac
    22741 cagcgactgg caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa
    22801 cagcgatgtc agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca
    22861 tcatctcctt actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc
    22921 agtacaatca gcaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg
    22981 ctcggtttgc cggggacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc
    23041 gattcaatca tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca
    23101 aaaccggcag caaacagcgg gaatttactg aagaagccgt tgaacggcgg tacgccggta
    23161 atcgccagcg ctgccacgca gaaacccacg cctggcaacg gcagggtgtg caatacgcca
    23221 cgcagacgcg gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa
    23281 aacaggcttt tagcgaacgc gtggttgacg atgtaggcga tgctgccctc cagcgccagc
    23341 cgcgagccga agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc
    23401 gaccaggcca gcaaccgctt catatcctgc tgtggcaaat acatcagaaa tccataaaga
    23461 atggtgacca gggccatgac catgccaacg ccgccaatca catgcgggat attgccgccg
    23521 tcgataatgg cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga
    23581 taggcgctga tcggtgttgg cgcttccatt gcgtccggta gccacgcttg catcggtagc
    23641 tgggccgatt tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg
    23701 ccgtgtaact cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg
    23761 gcggcaagat acaaccccaa cgaaccgata tgggtgataa gcaacgcttt tagtgctgaa
    23821 cgctgcgctt tatcgctctg ataataactg atcaacaccc aggagcagcc gcccgtaatt
    23881 tcaaaaaaca acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg
    23941 ataaacacca gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt
    24001 ttatccgtca gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca
    24061 aacagaatca gcgtacacac gcgatcaatg accaggccaa acaatgccac atcccccacg
    24121 ttgaccaacg tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac
    24181 aacatgcaca gcgtggtcag cgcggcgaac aaaacccccc attcggcggc ccgacgttgt
    24241 ggcgaaaacg aaacgaccag tgcgccgata aaaggcagca ataacgtcgt cagagcaaga
    24301 ttttccatat tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc
    24361 agcaaggctg aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag
    24421 cgtgttttct gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac
    24481 aagtgaagtc agcaggcagg cgagagaaag ttcttgcgcg cacccaaagg gcagaaacag
    24541 ggccacaaac agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgccagcgc
    24601 cagcccggca ccggaatatt ctgtcagcgg gccttcctgt aattcctgtt ctgcttcagc
    24661 aacatcaaag ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc
    24721 cagtacggtg gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat
    24781 atgcgtggaa cctgctatca gcgccaatac cagcagtgag agaatgagca ttggttcgac
    24841 cagaatgccg agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc
    24901 cagcccggaa agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag
    24961 atcgccgccg cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac
    25021 cagcatgctg ctgattaata cccacggcat caggcggaac atcagacctg aagatatagg
    25081 cgcaacttcc tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg
    25141 cccgcggcgg gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagcggggt
    25201 tagcatcagc agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag
    25261 agtttgtctc atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg
    25321 cgaccacata caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg
    25381 ccagccgttg ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg
    25441 tgctctgagc cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga
    25501 gctgttgtcg tagacgaaat agcgcagaga agaccacacg cagcggctgc atcacgccat
    25561 tgcctgacgg ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc
    25621 gacgaaacgc tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca
    25681 tcgccagtag taacagcaac agtaatgagg gggtgacctg tgtatgaaac gtgccgggta
    25741 caagtgctat tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatat
    25801 gcattatctt cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga
    25861 gcagtagcat ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtac
    25921 tgcgcggcgc accacagaaa gtaataccgt acattttgac gaagcacatt actgccagcc
    25981 caccagtgac tgccagcatc accatagcaa taggacccgc aagttgtagc gctacggctt
    26041 ccacacgact tagtgagaac agcgactgcc aggtgtacca ttcgctgata aaaccattca
    26101 gcggaggaag ggctgatatc gcgaggcaac caatcaggca tgctgcggct gtccacggca
    26161 tccgtttcgc cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg
    26221 cgcccgcgcc gagaaatagc agccctttga acagcgcatg gttgagcaga tgaaacagtg
    26281 cgccgagcag tccaaccacg gtgagcagcg ggtcgtgtag tgacagaccg accatcgcca
    26341 caccgactgc cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt
    26401 tgatatcctg ttccgccagc gcatacagca cgcccaggag cgcggagatt gcgccgatcg
    26461 ccatcaccag aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca
    26521 ctttcaggat gccgaaaata ccgattttga ccattacgcc agacatcaac gccgaagcgt
    26581 gcgatggtgc ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt
    26641 tcgcgccaaa accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg
    26701 aaagcgtgcg gaaactggca aaatctaggc tgccgctttc gcgccccatc agcaagaagg
    26761 cgatcataat cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga
    26821 tcgacgtttt atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa
    26881 acagcacgat gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga
    26941 aaatattcat aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag
    27001 tcaatgaata gagcgaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg
    27061 agaggctatc cattcgcagg gttaaatcgg caaagtcaaa aggggtggca aaacgggcaa
    27121 ctaaagttac gccttcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccca
    27181 ccagcgatgt gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg
    27241 aagccagact ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca
    27301 ttagcgttgc tcctgagtga gggaaagggg atcctccctg ccattggcaa accagcttgc
    27361 ggcaaggcgc tgtttttctt tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa
    27421 cgcctgattc gggcaagcgc gaacgcaggc aggaccttct ggcaagaaat cacacaggtc
    27481 gcatttcacc gcgacggtct ggacacccgg ttcccagcgc agcaaagcat gttgtgttgg
    27541 cgcgttttct tcgccgtctt tgagtgagcc ttcagcctga aaaacatatt gcgcaggggc
    27601 attcaccgga cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag
    27661 cttgcagcca atacagaggc tttcgttgag ttggatcgca tcatccctct gggagatggc
    27721 attgaccggg cagacctgca cgcaaggggc ttcctcacag tgatgacaca cgacaggggc
    27781 agtgagtgtt gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt
    27841 atgcacgtcc gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc
    27901 caccacaaag cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcataat
    27961 gcataaagga tgccagtatg attaatttaa tatattcaat aagttatgaa ttaactgaat
    28021 tctgaacggt acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt
    28081 gtcgcagggg aaagtgagaa tgtgatcttg atcacgataa gtgaggtgtg ctgaccgcaa
    28141 gcgcagccag cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac
    28201 aacgtcgtgg tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc
    28261 gtcaatcagg aagctgatgc gatgaatacc atcgtaggtt ttgcccatga aggacttttc
    28321 cccccagacg ccgaattgct cgcacacctg gtggtcctca tcagacagga gcgtaaagtt
    28381 aagcagctct ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag
    28441 cacatcaacg cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac
    28501 ggtacagccg ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa
    28561 gtcggtcaaa ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc
    28621 accggctttc agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg
    28681 acgacgttaa tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca
    28741 atatttgctg cgtccgcaga tgcggggctg tgggcagtaa tctgaatatg caactgcgca
    28801 gcccgttcat tttcagcagg ttgtgtgcgc gacaccagct ccgcaatgtt catatgatgc
    28861 gcgtcgaaaa gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga
    28921 acccagacag atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt
    28981 aaaagatcca gttcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc
    29041 catgaaccgg aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg
    29101 tcttcaatat tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg
    29161 cgatcggcac ccaacgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt
    29221 attcctttaa ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat
    29281 cagaacggtt ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt
    29341 ttgcacagag gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat
    29401 gaaaaaggta atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc
    29461 ggtacttcgg cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac
    29521 gaacatgctg atgtggtgat gatgacgctg gagctggctg acgggcgcat tccggtgatt
    29581 gccgggaccg gtgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac
    29641 agtggtatcg tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt
    29701 ttgtatcagc atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat
    29761 gtgccgtccc gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta
    29821 aaaaatatta tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa
    29881 gagctggttt cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc
    29941 atgcaattag gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg
    30001 gcccagatgt gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag
    30061 cgtctgatgc cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg
    30121 gcatgtaagg aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc
    30181 accgacagtg gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt
    30241 ttagggagat ttgatggctt actctgttca taagtcgcgc ctggcaacgg ttgcgggtgt
    30301 ttcgcttgtt ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag
    30361 tggtgatgaa gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat
    30421 gattttgccg gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt
    30481 cggtaaggcg ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg
    30541 tacccagttc acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct
    30601 gtggccgcag gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga
    30661 tgctggtcag acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca
    30721 gtatcgtggt cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt
    30781 taaactgctg aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg
    30841 ttacagcacg gagatgatga acgttatttc cgctggtctg gataaatctg ccactgacgc
    30901 cgcgaacgct gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga
    30961 caccggttta ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc
    31021 agcggcgctg gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat
    31081 ggccgtaact tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc
    31141 aggcctggca tctggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct
    31201 acagttcatc gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc
    31261 tgtcttccag gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt
    31321 tctgatatga tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc
    31381 acgaaatttg tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca
    31441 tccggcacac cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag
    31501 atgcaaaagc aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac
    31561 ccggacctgt tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt
    31621 gagcagtttg atcgcaaagg tatggtgaac aacaagttca actacttcat tatgagcaaa
    31681 ctggctgaag cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg
    31741 gtgaaaaagc tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct
    31801 ctggtgaaac gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg
    31861 ttcctgaaaa acgacgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt
    31921 ggctgggtaa gcaaagagaa cctggcacgc atgaaagagc tgacctacaa agcgaacgac
    31981 gtgctgaaaa aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt
    32041 ggtctgtaca aaggtgaagt ggtactgggc gatgagttct ccccggacgg tagccgcctg
    32101 tgggacaaag aaacgctgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc
    32161 ctgatcgagg cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc
    32221 tgttcatcat cttgccgtgc tgctggcacg gcaagacaac cattctcgta agatgtgcat
    32281 tgaacgctca ttcctccgcc atttcatccc gttttcctgt ggtaatcttc ctctgaacca
    32341 cctacgatca tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac
    32401 gtgaaagtga caatgttgaa gacaggcgca atagctccgg cggtccgtca atgggcggtc
    32461 ccggtttccg tctgccgagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg
    32521 caggctacta tggcgttgat ttaaccgggt tgatgaccgg gcagccggtt tctcaacagc
    32581 aatcaacgcg gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc
    32641 tggcaaccac ggaagacacc tggggacaac agttcgagaa gatgggtaag acctatcagc
    32701 aaccgaagct ggtcatgtac cgtggaatga cgcgtaccgg atgcggggcg ggccagtcca
    32761 taatggggcc gttctattgc ccggcggatg gtacggttta tatcgatctc tccttctatg
    32821 atgacatgaa agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgccc
    32881 atgaagtcgg tcatcatgtg cagaaactgt taggcatcga gccgaaagtt cgtcaactgc
    32941 aacaaaacgc gacgcaggcg gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg
    33001 actgttttgc cggtgtctgg gggcatagca tgcagcagca aggcgttctg gaaaccggcg
    33061 atctggaaga ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta caacagcaaa
    33121 gtcaggggcg agtagtacca gacagtttca ctcatggcac ttctcagcaa cgctacagct
    33181 ggtttaaacg tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca
    33241 tttaacttcc ggggcaggga tggctgaact gactgcgctt cacacattaa cagcgcaaat
    33301 gaaacgtgaa gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga
    33361 tcatgcgctt aagttacgtg atgccttacc tggcgactgg ctgtggattt cgccgcagcc
    33421 agatgctgaa aaccactgtt ctccctcggc actacaaact ttacttgggc gcgagttccg
    33481 gcatgcggta ttcgacgccc gccacggctt tgatgccgct gcctttgccg cacttagcgg
    33541 aacgttgaaa gcgggaagct ggctggtttt gttactccct gtatgggaag agtgggaaaa
    33601 ccaacctgat gccgactcgc tgcgctggag tgattgccct gaccctattg cgacgccgca
    33661 ttttgtccag catttcaaac gcgtacttac ggcggataac gacgctatct tctggcggca
    33721 aaaccagccg ttctcgttgg cgcattttac tccccgtact gactggcacc ccgcgaccgg
    33781 cgcaccacaa ccagaacaac agcaaatctt acagcagcta ctgaccatgc cgtcgggcgt
    33841 ggcagcggta actgctgcgc gtgggcgcgg taaatcggcg ctggcagggc aactcatttc
    33901 tcgtattgcg ggtagtgcga ttatcactgc gcccgcaaaa gcggcaacgg atgtactggc
    33961 acaatttgcg ggcgagaagt ttcgctttat tgcgcctgat gccttgttag ccagcgatga
    34021 gcaagccgac tggctggtgg tcgatgaagc cgcagccata cctgcgccgt tgttgcatca
    34081 actggtatcg cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggtac
    34141 cggacgtggt tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact
    34201 gcaacagccg atccgctggg cacagggatg cccgctggaa aaaatggtta gtgaggcact
    34261 ggtttttaac gatgaaaact tcacccatac accacaaggc aatatcgtca tttccgcatt
    34321 tgaacaaacg ttatggcgaa gcgagccaga aacgccgtta aaggtttatc agttattgtc
    34381 tggtgcgcac taccggactt cgccgctgga tttacgccgc atgatggatg caccagggca
    34441 acatttttta caggcggctg gcgaaaacga gattgccgga gcgctgtggc tggtggatga
    34501 ggggggatta tctcaagaac tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg
    34561 taatctggtg gcccagtcgc tggcggcgca cggcagcaat ccactggcgg cgacattgcg
    34621 tggacggcgg gtcagccgga tagcagttca tccggcgcgt cagcgcgaag gcgttgggca
    34681 acagctcatt gccagcgctt tgcaatatag gcctggcctc gactatcttt cggtgagttt
    34741 tggttacacc ggggagttat ggcgtttctg gcaacgctgc ggttttgtgc tggtgcgaat
    34801 gggtaatcat cgtgaagcca gcagcggttg ctatacggcg atggcgctgt taccgatgag
    34861 tgatgcgggt aaacagctgg ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc
    34921 tctcgcgcag tggaatggcg aaacactccc tgttgatcca ctaaacaatg ccgtcctttc
    34981 tgacgacgac tggcttgaac tggccggttt tgctttcgct catcgtccgc tattaacatc
    35041 gttaggttgc ttattgcgtc tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg
    35101 gcgtttacag aaaaacgtca gcgacgcgca gttatgtacc acacttaaac tttcaggccg
    35161 caagatgtta ctggtccgtc agcgggaaga ggccgcacag gcgctgttcg cacttaatga
    35221 tgttcgcact gagcgtctgc gcgatcgcat aacgcaatgg caattttttc actgactcct
    35281 tcaattttct cccatggtca ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg
    35341 agttcgccat gaaacatgac cattttgttg ttcaaagccc ggataaacct gcgcaacagt
    35401 tgttactgct tttccacggt gtcggggata acccggtggc aatgggggaa ataggtagct
    35461 ggtttgcacc gttgttcccg gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg
    35521 gtaatccggc ggggcgtcag tggttttcgg tgcagggtat tacggaagat aatcgccagg
    35581 cgcgtgtgga tgccatcatg ccgacgttta ttgaaacggt gcgctactgg cagaaacaga
    35641 gcggagtagg ggctaacgcc acggcgctaa tcggtttctc tcaaggcgcg attatggcgc
    35701 tggagagcat taaagccgaa ccgggccttg cttcacgcgt cattgctttt aacggacgct
    35761 atgcgagcct gccggaaacc gcgtctacag ccacgacaat tcacttgatt catggcggtg
    35821 aagatccggt tatcgatctg gcgcatgcag ttgcggcgca ggaagcgtta atcagtgccg
    35881 gtggtgacgt gacgctggat atcgtggagg atttaggcca tgcgatcgac aatcgcagta
    35941 tgcaatttgc cctggatcac ttgcgttata ccattccgaa acattatttc gacgaagcgc
    36001 taagcggcgg taagcctggc gatgatgatg tgattgaaat gatgtaataa tagccgggta
    36061 ggcctgataa gaccgtatca ggcatcatgg ccggatgcgg cgttaacgcc ttatccggcc
    36121 acttagctta cttcttcggt tgatcctttt tcggccagtc gtcgtcatcg tcccacttat
    36181 cgttgaaatc acgatgcggg ggaagctccg gcttgttggc gagaaatttc ttatggtcga
    36241 cgcgcttgag atctttaatc acgttcagca ggacgcctac caaaaagaca atcaccagaa
    36301 tccaccaata tttagccagc cagtccatgc ttatttcctc ttgcagaacc actcatcacg
    36361 cgacgagctg ttccatgata cgttgataca tacgggcaag tagctgcagg tcggcagcgt
    36421 tcacacattc attaatttta tgaatagtgg cattgaccgg cccgagttcc accacctgcg
    36481 cccccatgcg ggcaataaag cgcccgtcgg acgttccgcc tgtggtcagt agctgcggtt
    36541 taatttcatt atagtgctca accgcgttaa cgaccgcatc caccagttta ccgcgcgcgg
    36601 tcaaaaatgg ctgcccggaa agccaccaat ccaccgtata gcgcagttga tgtttttcaa
    36661 gcagggcaag cacctgcgct ttgatcatct catcagtcag ttcggtgctg aagcggaagt
    36721 taaactgcac aaacagttca cccggaataa cgttgttact gcccgttccc gcctgaatat
    36781 tggcaatctg catactggtc gccgggaaga attcattgcc ctgatcccac tcaatagcca
    36841 ctaattcatt aaggaaaggt gctgcgcgat gtaccggatt gtcagccaga tgtgggtagg
    36901 caacatgccc ctgaacgcca tgaatggtta ggttgcaggt taacgatcca cgacgaccat
    36961 ttttcaccac atcacctacc acttcgatac tcgacggttc gccgaccagg cagtaatcga
    37021 gacgctcatt acgtgccatt aacgcttcga cggcttttac cgtaccattg tgggcactgg
    37081 cttcttcatc agaggtgatc agaaatgcca gtcgccctgt atggttggga tgttgtgcga
    37141 caaaacgttc tgcagctacc accatcgccg ccagcgagcc tttcatatct gccgcaccgc
    37201 gcccgaataa catgccgtca cgaatggttg gttcaaacgg cggattgatc caacgatcgg
    37261 cgtcgccagg cggcaccacg tcggtatgcc cggcaaaggc caatgtttca ccttgcccac
    37321 gccatgccca aaaattctgc gtatcggcaa agtccatgcg ttcaacggta aaaccgatcg
    37381 cctgcaaacg ttcaatcaac aaagcctggc atcctgcgtc atcaggactc agggaagggc
    37441 ggcgaataag ctgttgtgtc agctcaataa ccgggcacga catagactac acctcatgga
    37501 aaaatttctg ataactggaa tcactgaaac ccagcagcat aggcttacca ggcgcgcaga
    37561 gcaatggacg tttgataatt gcaggcattt cagtcattaa tgccgccgca gaggccgcat
    37621 cggtgattat attgcgggtg gtttcgtcca gtttacgcca ggttgtacca cgggtgttga
    37681 gtaacgcttc ccagcctaat tcgttgataa aaccgttcaa taattcgctg tccagcccat
    37741 cgacgcggta atcatgaaaa cgatagtcga tgttattggc ttccagccaa cggcgagcct
    37801 ttttaatggt gtcacaattt ttgatgccgt aaagtgtaac cataatgaat cctttttcga
    37861 tttatgagaa taaatactca tttaagagtg aaaacatttt gcctgcaaat gattatcagg
    37921 aaatataaca ttatcgaagt attcaaatta acagctatta aataatatta aacattttta
    37981 acgtaaacat taagaatatt catcttgtta tctgtctgta ttttaaccag aattaaatca
    38041 gtattagatg ccgtatgaat atctcgaaat gttgctgtgt atcacataca aatcatcact
    38101 gcaggaccat agtatttaca taaggtttgt cccccggagg tagtcatcgc agcaatcgag
    38161 taaattagct tcaccacggt atattgtttc cgtagaatac ccataattat aagagaggtt
    38221 gttatgattg aacgtgaact ggggaactgg aaagacttta ttgaagttat gcttcgtaag
    38281 taattcctga gacaaagtac aaacagcaag aacccgctga gcaggttctt aatcggaaag
    38341 ttgaaaaagg cgacacattg gcatgtcgcc ttttttattg cttattccgg gcgcggcttc
    38401 agcgggaagc ggcggcgcac cagcacaaag aacagcggca cgaagtaaat agccagaata
    38461 gtggctgaaa tcatcccgcc cattacgcca gtacccaccg catgctgacc accggaaccg
    38521 gcacccgtgc tggttgccat tggcaatacg ccgaagataa atgccagcga ggtcatcaga
    38581 atcgggcgta aacgctgacg gcaggcgtgg agcgtcgctt caaacaggtc gtggcctttt
    38641 tggttcatct cgttagcaaa ctcgacgatc aggatggcgt ttttcgccga taaaccaatg
    38701 accgttaaca ggcccacctg gaagtaaacg tcgttttcca gcccgcgcat ccaggttgcc
    38761 agcagcgcgc cgattacccc cagcggcacg accagcatta ccgagaacgg taccgaccag
    38821 ctttcataca atgcagccag acacaggaat accaccagca aggaaatggc gtacagcgcc
    38881 ggagcctgcg cgccggaaag ccgctcctga tacgacatcg ccgtccactc cagaccaaag
    38941 ccgtttggta gctgcttcac taacgattcc ataatatcca tcgccgtacc ggtactgacc
    39001 cccggtgcgg cttccccaac aatttcaacc gcagaataac cgttatagcg ttccagacgc
    39061 ggcgagcctg tctcccagcg tgaggtcgcg aaagcagaga agggcaccat gccgccatct
    39121 ttatttcgga cataccagag attgatgtca tctggcagca tgcgatacgg cgcagctgcc
    39181 tgcacataga ctttcttcac gcgaccgcga tccataaagt cattcacata gctcgaaccc
    39241 caggcggttt gcagtgtgtc gttaatatcg tcgatagcaa cgcccagcgc ctgagcttta
    39301 cgctggtcga tatcaatctg caactgcgga ctgtcatcga ggccgttatg gcgcacacgg
    39361 gttagctccg ggttttccgc cgccagcgcc agcaactggt tacgtgctgc catcagcgca
    39421 tcgtgacctg ctccagcgtg gtcctgcaac tccatatcaa aacctgcaga actaccaaga
    39481 ccgctaattg ctggcgggct gctggcgata acgcgagctt ctttaatttg gttaaacgcc
    39541 tttgttgcac gctcgataat ggcaaacgag gtgccggtct tactgtcgcg ttcgctccag
    39601 tctttcaggc ggataaacat tcgcgccacg ttttgcccgt tacccccagg gccagaacca
    39661 acagtggcaa acaccgacat gatgttgtct ttttcatggg tgaagtaata tttctcgatt
    39721 tgctcaacga ctttcagggt ctgttgttgg gttgaaccgc tgggcaattg taccgaggta
    39781 gtaaacatgc cacggtcttc cagcggtaag aacgacgtcg gcaaacgcag gaacaggaac
    39841 accatgccgc caagcagcag gacataaatc acaatccagc gcaggctacg gtggagaatt
    39901 ttcgccaccc ctttttcgta gcgttcggcg ttgcggttaa acatctggtt aaaccaggca
    39961 aaaaagcctt tttgcccgtg atgctcacct tttttcagtg gcttaagtag tgtggcacac
    40021 agagccggag tgaggatcat cgctaccagt actgacagca ccatcgccgc aacaatggta
    40081 atagagaact ggcgatagat ggcaccggtg gtgccgccga agaaggccat tggtacaaat
    40141 accgccgaaa gcaccatcgc aatcccgacc agtgccccct ggatctgccc catcgattta
    40201 cgcgtggctt cgcgaggagt gagtccttcc tcactcataa tacgttcgac gttttccacc
    40261 accacgatgg cgtcatccac cagcagaccg atcgccagca ccatcgcgaa catggttaag
    40321 gtgttgacgc tgtaaccgaa ggcgtaaagt acggagaagg ttcccatcaa caccaccggc
    40381 acggcgatag tgggtatcag cgtggcgcgg aagttttgca ggaacagata cataacgagg
    40441 aaaaccagag cgatagcttc cagcagcgtt ttcaccacgt cttcaatgga ggctttaaca
    40501 aacgaggtgg tttcatacgc caccttgtat tccagtccgt gcgggaaata ctgcgccagc
    40561 tcgtcgagac gattgagcac cagctccgct gtcgccattt cgttcgcgcc ggaggccagt
    40621 tttaccccca acccggaggc tggcttacca ttgaagcggc taagataatc gtatttctcc
    40681 gcccccattt cggcggtggc gacatcgccg agccttactt ctgagccgtc ctgattcacc
    40741 cgcaaggtga tatcgcggaa ctgttctggt gtttgcagca gtgactgggc gttaatggtg
    40801 gcgttgagcg cctgcttatc gacggaaggt gtaccaccaa gttgcccaac cgcaatctgc
    40861 gcgttctgtg actcaatggc atcagtgaca tctttagctg tcatctggaa actgttgagt
    40921 ttcgccgggt ccagccagat acgcatggaa tattgcgaac cataggcatc gatatccccg
    40981 acgccattta cgcggcttaa cgggtcctga atattactgg caacataatc agcaatatcc
    41041 tgtttatcca tcgaaccatc ggtagagacg aaggcaatgg tcagaatgtt ggtatcgccg
    41101 gttttacgca cagtcacgcc ctgattttgc accgcctgcg gtaactttcg catggctgat
    41161 tgcagctggt tttgtacttg ctgcacggct tcgtccggat cggtgcctgc tttaaaactt
    41221 aaagtgacag atgcctgacc ggtgccactg ctctgagatg acatatacat gagattatcg
    41281 aggccggtca tattttgctc gataacctgg gtcacggtgt tttccagcgt ctgggccgat
    41341 gcgccgggat agttagcggt cactcgcaca ttcggtggcg cgagatcggg gtattgttca
    41401 acgggcaatg aaaaaatcgc cagggtacct gtcagacaca acaggattgc cagcacccag
    41461 gcaaaaatgg ggcgatcaat aaagaaattc gccattaaaa gaggacctcg tgtttctgcg
    41521 tatcgttgta ggcttgactt gcttcactgt agcggcaagg tacgagccaa tcgtggagaa
    41581 aaaaaggagt taatgtaaat tatcatgccc ttttatactg aaccttaact gcaagtattc
    41641 tttggtcagt aggaggcatt acatcaactg actttcctca ccctccgcag agcgaaagct
    41701 aatactcact aacgtgccgc cgccggaagg ttgtgaaaaa gtcagcgtcc cacctagccg
    41761 ttccgcgcgt tcgcgcatga tattcagacc ataatgacct tcgggttctt tcggttcacc
    41821 gataccaatc ccgttatcac ggatataaac cgtgtgattg ccgtccggcg cggtgacgca
    41881 actgacggcg atttcgctgg cgttggcgtg cttcatcgca ttcagcaccg cttcgcgaat
    41941 aatttgcaac aaatgcacct gcatttgcgc atccagtgcc agggttggca gacggcagtc
    42001 gagggtcagt ttggcgctgg tttgattttg taacgtatcc agcatttccc tcaacgcgga
    42061 ggggagatcc gcctgctgca gcgtcaggcg aaaagtagtc aacagctcgc gtaactgccg
    42121 ataagcatca ttcaacgcct gggaaaaatc ggccatgata ctttgtgcgg tggcgttatc
    42181 ttccggtatc gaacgcttaa gtaacgtcaa ctggatacgt aagtaagaaa gtacctgagc
    42241 cagcgagtcg tgcaattcgc gggcgatggt tgcacgttct tccatcaaca ataactgctg
    42301 aaaatgcttc tgcgcctgat taaagtacaa accgcgtccc agcatcgacg aaacgctgtt
    42361 aagcagcggt tctgatgatg aaacgtgact attttgccag tgcagttcgc cgtaaaccgt
    42421 ctcttgcatt gtcaccggta aaatctgcat cggcaattcc gggtttggtt gcccttcgct
    42481 aatccgccag ttttcaccga catttaactc cagatattca gccgcttcat tgtcgcgaac
    42541 aatctgcaaa atatggcgga aacaatgcac atcaatctgg ctggtgttca gcgcctgcga
    42601 acattgatac aacacctcca gacgacgctt ggcctcgtgg agatcgcggg tcttttcttc
    42661 tactgacgcc tccagcgaac ggtacaattt atgcagctcg ctcgacatct ggttaaaggt
    42721 ttttgcaagt agaccaagct cattcggcag gctggtatcc agcggtggcg agtcgaactg
    42781 tccgtgttca atacgctgac tggcggtaac cagctgattc agcggggcaa ccacctgatg
    42841 gcgtatgcgg cccaaagtaa aaaagaccag cgtgaaaata ccgataccgc cagccaggga
    42901 aatcgccacc accagcagca ttttgcgttc agcgtagtgc tgtaaagcca gcacgaacag
    42961 gtctatctga ttaacataat tattaatatt ggcctgatac cacggcaaat cgcccttgct
    43021 gagccgatta ttcatctcca gccagttggc attcagatgc gcatagcgag tttttactgc
    43081 ttctggcaca taccagacgt tgaggttggt taataccggt gaatgcagtg cctgctgaaa
    43141 cagctggcga tgtgcattga gttgtggagt gccactttgc aagtcgtagc ccaggcggta
    43201 actctgcata cgcagcgatc cggcaatatt gatagcctca gcgtcgcgca aactgctcgc
    43261 cagagttagc agagcgatac ccgtggaaag aatcgacagc agcacaatgt aaaaaaaggc
    43321 ccgggccaga ctggccgaga cgggtcgttt aacaatcaca cgcgtcttct ccacaaaaaa
    43381 tcattaatgt tccagttcgc tgaaacgcaa tttagttagt gatgggatta tttgatctgc
    43441 cacagtttat agcgaaatag ttacttattc tgggcacatg aacattgccg attcccctgt
    43501 aagtcgggta ataacaacac tcatataaag aataaggttt ttacaaccaa aaaagaaggt
    43561 cgttatgaat cgttttatta tggccaacag tcagcaatgt ctgggttgtc atgcttgtga
    43621 aatcgcttgt gtcatggctc acaatgatga gcaacatgtc ctgagccaac accattttca
    43681 tccccgaatt acggttatca aacatcagca gcaacgtagt gcagtgacct gtcaccattg
    43741 tgaagatgcg ccctgcgccc gtagctgccc taatggcgca atcagccacg ttgatgacag
    43801 cattcaggtc aatcagcaaa agtgtattgg ctgtaaatcc tgcgtggtgg cctgtccgtt
    43861 tggtacgatg caaatcgtcc tgacacccgt cgcagcgggc aaagttaaag ccacggcgca
    43921 taaatgcgac ctttgtgcag ggcgcgaaaa cggtcctgcc tgtgtcgaga attgcccggc
    43981 ggacgcgctg cagctggtca ctgacgccgc actctcaggc atggcgaaat cccgccgctt
    44041 gcgtaccgca cgtcaggaac atcaaccgtg gcatgccagt accgctgcac aagaaatgcc
    44101 ggtaatgagt aaagtcgaac aaatgcaggc aacgcccgcg cgtggcgagc cggataaact
    44161 ggcgattgaa gcgcgcaaaa ccggttttga tgaaatttat ctgccattcc gtgccgatca
    44221 ggcacaacgg gaagcctcgc gctgccttaa atgcggcgag cacagcgttt gtgaatggac
    44281 ctgcccgctg cataaccata taccgcagtg gattgaactg gtgaaagccg gaaacatcga
    44341 cgccgccgtc gagctttctc acctgaccaa caccctgccg gaaattaccg gacgcgtttg
    44401 tccgcaagac cgtttgtgtg aaggtgcctg tactattcgc gatgagcacg gtgcggtaac
    44461 tatcggcaac attgaacgct acatttcaga tcaggcgttg gcgaaaggtt ggcgtcctga
    44521 cttaagccat gtcaccaaag tggacaagcg ggtggcgatt atcggtgcag gtccggcagg
    44581 gctggcctgt gcggatgttc tgatccgcaa tggcgtggcg gtgacggtgt acgatcgcca
    44641 tccagaaatc ggtggcttgc tcactttcgg cattccttct ttcaaactgg ataaatccct
    44701 gctggcacgt cgtcgggaaa tcttcagcgc gatggggatt cacttcgaac tcaattgtga
    44761 agtgggtaaa gatgtctctt tggattcgct tttggaacaa tacgacgcgg tcttcgttgg
    44821 cgtaggcact taccgttcca tgaaagcggg tttacccaat gaagatgcgc cgggcgttta
    44881 tgacgcgctg ccgttcctca tcgccaacac taaacaggtg atggggctcg aagagctacc
    44941 ggaagagccg tttatcaata ccgccggact taacgtcgtg gtactgggcg gcggcgacac
    45001 cgcgatggac tgtgtgcgta ctgcgctgcg ccacggcgcg agtaacgtca cctgcgctta
    45061 tcgtcgtgat gaagctaaca tgccaggctc gaagaaagag gtgaagaacg cccgcgaaga
    45121 gggggccaac ttcgaattta acgtccagcc ggtggcgctt gagctgaatg aacaaggtca
    45181 cgtctgcggg attcgtttcc tgcgcacgcg tcttggcgag ccggatgccc aggggcgtcg
    45241 gcgtccagtg ccggtggaag gcagtgaatt tgtcatgcca gccgacgcgg tgattatggc
    45301 gtttggcttc aatccgcacg ggatgccgtg gctggagtcg cacggtgtaa cggtagacaa
    45361 atggggccgc atcatcgcgg atgtggaaag ccagtaccgt taccagacca ccaatccgaa
    45421 aatcttcgcc ggtggcgacg ccgtgcgtgg tgcggatctg gtggttaccg caatggcaga
    45481 aggacgtcat gcggcacagg ggattattga ctggctgggg gtaaaatcag tcaaatctca
    45541 ctgatagcct gcgcagacaa acccgacttc acagcgtaat ataattgttc atttcgcgct
    45601 gtggagtcgg tatgacgcaa caaatcaccc tcattaaaga caaaattctc tccgataact
    45661 atttcaccct gcacaacatt acttacgatc tcacgcgcaa agatggcgaa gttatccgcc
    45721 ataaacgtga agtttacgat cgcggtaatg gtgcgacgat cctcctgtac aacgcgaaga
    45781 aaaagaccgt ggttctgatt cgtcagttcc gtgtcgctac ctgggttaat ggcaatgaaa
    45841 gcgggcagct gattgaaacc tgcgccgggc tgctggataa cgacgaaccg gaagtgtgca
    45901 ttcgcaaaga agcgattgaa gagacgggct atgaagtggg cgaagtgcgc aaattatttg
    45961 aactgtatat gtcgccaggc ggtgtgactg agctaatcca cttttttatc gccgaataca
    46021 gtgacaatca gcgcgctaac gccggtggcg gtgtcgaaga tgaagatatt gaagtgctcg
    46081 agctgccgtt cagccaggcg ctggagatga tcaaaaccgg cgagatacgt gacggtaaga
    46141 cggtgttatt gcttaactat ttgcaaatgt cacatttaat agactgaaaa ataacaataa
    46201 tatttcgttg tttattcttg gctcaatccg acaaatccga ttgagccgcg ctacctgcgc
    46261 aacgaagata cgacttgtgc tgtttgtttg aacttctggg gtcgtaccgt ccatgcgcta
    46321 tcgcattttc cttctctttt ttttcgcttt gttgccgacg tctttggtgt gggcggcacc
    46381 agcgcaacgg gcgttttccg actggcaggt cacctgtaat aaccaaaact tctgcgtggc
    46441 gcgtaatacg ggcgatcata atggactggt gatgaccctg agccgcagcg ccggggcgca
    46501 taccgatgcc gttttacgta ttgagcgcgg cgggttgaag tcgccggatg cgtctgaagg
    46561 ggaaattgcg ccacggctgc tgttagatgg cgagccgttg gcgttaagtg gtgacaagtg
    46621 gcggatttca ccatggttat tagtaaccga tgatacggca accatcaccg cgtttttgca
    46681 gatgattcag gaagggaagg cgatcacctt acgcgacggc aatcagacca tttctctgag
    46741 tgacttaaaa gcggcgttgt tgtttattga tgctcagcaa aagcgcgttg gcagtgaaac
    46801 cgcgtggatc aagaaagggg acgaaccgcc gctcagcgta ccgcccgcgc ctgcgctgaa
    46861 agaggtcgtg gtggttaacc caacgccgac gccactctca ctcgaagaac gcaacgattt
    46921 gctggattat ggaaactggc ggatgaatgg tctgcgctgc tcgcttgatc cattgcgtcg
    46981 tgaggtgaat gtcactgcgc tgactgatga taaagcgctg atgatgatta gctgtgaggc
    47041 aggggcctat aacaccattg atttggcatg gattgtgtcg cgtaaaaagc cgctggcctc
    47101 gcgcccggtc cggctgcgtt tgccgttcaa caacggtcag gagacgaatg aactggaact
    47161 gatgaacgca acatttgatg agaaatcgcg tgaactggtg accttagcga aagggcgcgg
    47221 attaagcgat tgtggcattc aggcgcgctg gcgctttgac ggtcagcgtt ttcgcctggt
    47281 gcgttacgcc gcagaaccca cctgcgataa ctggcatggg ccagacgcct ggcccacgtt
    47341 gtggatcacc cgttaaacaa aatggcggga catgtaggcc ggataagacg ctgtaggccg
    47401 gataagacgc gtcagcgtcg catccggcaa tcagcatccg gcgatcacca tcaggcacct
    47461 tttactccca gcaccttatg cgcttttgcc acaatattct cggcggtaaa gccaaagaac
    47521 gggaacagct tatccgccgg agcagattcc ccataacccg tcatcccgac aattgcccct
    47581 ttcagaccaa catacttgta ccagtaatcg gcaatacctg cttccaccgc cacgcgagcc
    47641 gcaacgttag aaggcaacac cgactcccga tattcctcat cctgggcgtc gaaaatatcg
    47701 gtcgagggca gggaaaccac gcgaacattg cgaccttctc ccgctaattt ctccgccgct
    47761 tgcagggtga tttccatctc tgaaccggtg gcaatcaaaa taatatctgg cttaccgccg
    47821 ctgtctttca gcacataacc gccacgagca atctctttaa cctgatccgg cgtacgttcc
    47881 acctgggcca gattctgcct ggagagaatc agtgccgtcg gtccgttgtg gcgctcaacc
    47941 gccagcttcc agcccaccgc cgcttccacc tgatcgcacg gtcgccaggt gctgaaattt
    48001 ggcgttaagc gcaggctggc cagttgctca acagcctggt gcgtcggacc atcttcgccc
    48061 agaccaattg agtcgtgggt ataaaccata atctgccgcg ctttcatcag tgccgccatc
    48121 cgcgcggcgt tacgggcgta ttcaacaaac atcaggaacg ttgcggtata cggcacaaag
    48181 ccgccgtggt gcgcaatgcc gttggcgata gcggtcatgc caaattcacg caccccgtag
    48241 tgaatgtagt tgcccgccgg atcttccttc agcgaaacag aacctttcca gatggtcagg
    48301 ttgctgggag ccagatccgc cgaaccgccg agcagctccg gtagcatcgg cccgtaagcg
    48361 ttaagcgtat tttgcgaagc cttacgggta gcgattttcg ccggattcgc ctgcaactca
    48421 ttgatatatt tctgagtcgt tttctcccag tccttcggta aaccaccgct catccgacgg
    48481 gtaaactctt ctgccagttg cggatgagcc tttttatagg cggcaaactt ctcattccag
    48541 ctctgctgcg ctttttcgcc tttttcgcgg gcatcccaag cgtgataaat atctttaggg
    48601 atctcaaatg gcggatggtg ccagcccagt ttttgccgtg ccagcgccac ttcttcttcc
    48661 cccagcggtg cgccgtgcgc ctcttcctta cctgctttat tcggcgaacc aaagccaatc
    48721 accgtacggc agataatcag cgacggctta tctttcacgc tttgcgcttc aaggattgct
    48781 tccttcaccg cctgcggatc gtgaccgtcg atttcatgga tcacatgcca gtgataggct
    48841 tcaaaacgtt ttgccgtatc gtcggtaaac cagccttcgg tttctccatc aatcgaaata
    48901 ccgttgtgat cgtaaaaacc aatcagcttg cccagtccca gcgtgcccgc cagcgaacag
    48961 acttcgtggg aaataccttc catcaggcag ccgtcgccca taaacacata ggtgaagtga
    49021 tcgacaattt catgatccgg ctggttaaac tgcgccgcca gtgtacgttc cgctatcgcc
    49081 agcccgacgg cgttcgccaa accttgtcca agaggaccgg tggtggtttc aactccgggc
    49141 gtatagccga tttccgggtg gccaggggtt ttcgaatgca gttgacgaaa gttcttcagc
    49201 tcttccagcg gcaggtcgta accggtcaga tgcagcaaac tgtagagcag catcgacgcg
    49261 tgaccgttgg aaagaataaa gcggtcgcga tcataccagg ttgggtcggt agggttatgt
    49321 ttaagaaaat cgttccacag cacttcggca atatcagcca tgcccatcgg cgcgccggga
    49381 tgaccagaat tggctttttg taccgcatcc atactgagtg cgcgaatcgc attggcaagg
    49441 tcttttcggg acatataaca ctccgtggct ggtttatagt ttggcggcaa gaagatcttc
    49501 cagtttgcgt tgatcaacgg cgaacagacg aatgccttca gacagttttt ctaccgccat
    49561 cgcatcctga ttgtgctccc aacggaactc cgcttcgctc atgggagctg ggcgtgggaa
    49621 cgtctgagaa ggtgggatta atttacgtac cactggcgaa actttttcct gcagctcctt
    49681 cagcaaattc ggtgcgatag tcagtcgatc gcagccggtt aaggcgagga tttgttcggt
    49741 gcgacggaag ctcgcgccca tcacaatggt ttcatagtgg tgttgcttat agtagtcgta
    49801 gatattgcgc accgatttaa cgcccggatc ttcttccacc acatatgggt ccatcggctt
    49861 gcgtgcctga taccagtcat aaatacgccc gacaaacggc gaaatcagaa aaacgcccgc
    49921 ttctgcacag gcccgcgcct gtgcaaaaga aaacagcagc gtcaggttgc agttaatacc
    49981 ttctttttcc agctcttctg ccgcgcgaat tccttcccag gtcgaagcca gtttgatcag
    50041 aatgcgtgat ttctcaacgc cttgttgctg atacaagtcc accagatggc gcgccttctc
    50101 aatactcttt tctttatcaa aagagaggcg tgcatcaact tctgttgaca cgcgaccggg
    50161 tacgattttg aggatttcag caccgaaatt gaccgccagt ttgtcacacg ctgcgaccac
    50221 ctgttgttcc tgggtcttgc catttttttt accccaggcg atagcatcgt ctattaaatg
    50281 ctcatattgt gataatccgg cagccttgag taacagcgaa ggattggtgg tggcatcctg
    50341 gggatgataa tggcgaatgg actcaatatc gccgctgtct gccacgacag tggtgaactg
    50401 tttgatgccg tctaactcgt tcataggaaa tactccttga aaagtaaagt gttagatgag
    50461 tgcgttaatt cacacttctg agaaatttcg ctaaacgcat caaaaaagca tagcagacag
    50521 gcatggtatt gctggattaa gcaggtaaca tcagtgttat aggattatta ccaaaacatt
    50581 atattaatgg tgagagtttg gacttgctca aagtctgtag actccggcaa ggtaataatg
    50641 tgcgccacgt tgtgggcagg ggctgttgcc cacacacttt atttgtgaac gttacgtgaa
    50701 aggaacaacc aaatggatga ccagttaaaa caaagtgcac ttgatttcca tgaatttcca
    50761 gttccaggga aaatccaggt ttctccaacc aagcctctgg caacgcagcg cgatctggcg
    50821 ctggcctact caccaggcgt tgccgcacct tgtcttgaaa tcgaaaaaga cccgttaaaa
    50881 gcctacaaat ataccgcgcg cggtaacctg gtggcggtga tctctaacgg tacggcggtg
    50941 ctggggttag gcaacattgg cgcgctggca ggcaaaccgg tgatggaagg caagggcgtt
    51001 ctgtttaaga aattcgctgg gattgatgta tttgacattg aagttgatga actcgacccg
    51061 gacaaattta ttgaagtcgt cgctgcgctc gaaccgacct tcggcggcat caacctcgaa
    51121 gacattaaag cgccagaatg tttctatatt gaacagaaac tgcgcgagcg gatgaatatt
    51181 ccggtattcc acgacgatca gcacggcacg gcaattatca gcactgccgc catcctcaac
    51241 ggcttgcgcg tggtggagaa aaacatctct gacgtgcgta tggtggtttc cggcgcgggt
    51301 gctgcagcaa tcgcctgtat gaacctgctg gtagcgttgg gtctgcaaaa acataacatc
    51361 gtggtttgcg attcaaaagg cgttatctat cagggccgtg agccaaacat ggcggaaacc
    51421 aaagccgcat atgcggtagt ggatgacggc aaacgtaccc tcgatgatgt gattgaaggc
    51481 gcagatattt tcctcggctg ttccggcccg aaagtgctga cccaggaaat ggtgaagaaa
    51541 atggctcgag cgccaatgat cctggcgctg gcgaacccgg aaccggaaat tctgccgccg
    51601 ctggcgaaag aagtgcgtcc ggatgccatc atctgtaccg gccgttccga ctacccgaac
    51661 caggtgaaca acgtcctttg cttcccgttc atcttccgtg gcgcgctgga cgttggcgca
    51721 accgccatca acgaagagat gaaactggcg gcggtacgcg cgattgcaga actggcccat
    51781 gcggaacaga gtgaagtggt ggcttcagcg tatggcgatc aggatctgag ctttggccct
    51841 gaatacatca ttccaaaacc gtttgatccg cgtctgatag tcaagattgc tcctgcggtc
    51901 gcgaaagcgg cgatggagtc gggcgtggcg acgcgtccga ttgctgattt cgacgtctac
    51961 atcgacaagc tgactgagtt cgtttacaaa accaacctgt ttatgaagcc gattttctcc
    52021 caggctcgca aagcgccgaa gcgcgtagtt ctgccggaag gggaagaggc gcgcgttctg
    52081 catgccactc aggaactggt aacgctggga ctggcgaaac cgatccttat cggtcgtccg
    52141 aacgtgatcg aaatgcgcat tcagaaactg ggcttgcaga tcaaagcggg cgttgatttt
    52201 gagatcgtca ataacgaatc cgatccgcgc tttaaagagt actggaccga atacttccag
    52261 atcatgaagc gtcgcggcgt cactcaggaa caggcgcagc gggcgctgat cagtaacccg
    52321 acagtgatcg gcgcgatcat ggttcagcgt ggcgaagccg atgcaatgat ttgcggtacg
    52381 gtgggtgatt atcatgaaca ttttagcgtg gtgaaaaatg tctttggtta tcgcgatggc
    52441 gttcacaccg caggtgcaat gaacgcgctg ctgctgccga gtggtaacac ctttattgcc
    52501 gatacctacg tcaatgacga accagacgca gaagagctgg cggagatcac cttgatggcg
    52561 gcagaaactg tccgtcgttt cggtattgaa ccgcgcgttg ctctgttgtc gcactccaac
    52621 tttggttctt ctgactgccc gtcgtcgagc aaaatgcgtc aggcgctgga actggtcagg
    52681 gaacgtgcgc cagaactgat gattgatggt gaaatgcacg gcgatgcagc gctggtggaa
    52741 gcgattcgca acgaccgtat gccggacagc cctttgaaag gttctgccaa tattctggtg
    52801 atgccgaaca tggaagctgc ccgcattagt tacaacttac tgcgcgtttc cagctcggaa
    52861 ggtgtgactg tcggtccggt gctgatgggc gtggcgaaac cggttcacgt gttaacgccg
    52921 atcgcatcgg tgcgtcgtat cgtcaacatg gtggcgctgg ccgtggttga agcgcaaacc
    52981 caaccgctgt aattcttttt taactctcac gcttaccctg aatattcagg gtaagcggtt
    53041 tagctgcaat atattagtaa agcttattac tgagtttgcg aataataaaa aaagcagtat
    53101 ataaaatatc tcgatattat tttatttata ttcatgcgtc gcatatgaaa gtttattcat
    53161 aacagtaaat atctctccat tcttagtgat ctacctcacc ttttaaacgc gcttgccgaa
    53221 ttttgttatt tactctgacg aaaaattgtc acgatacacg aaagtttttc acaggcggcg
    53281 actcatggat aaagaacgca tcattcagga atttgtgccc ggcaaacagg tcacactggc
    53341 gcatctcatc gcgcatcccg gcgaggaact ggcgaagaaa atcggcgttc cggatgcggg
    53401 cgcaattggc attatgacgc taactcccgg cgaaacggcg atgatcgccg gtgatttagc
    53461 gctgaaagcc gccgatgtgc atatcggatt tctcgaccgc ttcagtggtg cattggtgat
    53521 ttatggctcg gtgggcgcag tagaagaagc gttatcacaa accgtcagtg gtttaggtcg
    53581 cttattaaat tatacgcttt gtgaaatgac caaaagttaa tacgaggcat tcatgaaacg
    53641 aattgcgttt gtgggtaccg tcggggcagg gaaaacaacg ttatttaatg cgttgcaggg
    53701 gaattatacc ctcgccagaa aaacacaggc cgtggaattt aatgataagg gcgatattga
    53761 tacgccgggt gaatatttta gccatccccg ctggtatcac gccttaatta ccacgctaca
    53821 ggatgtggac atgctgatct atgtccacgg cgcgaatgac ccggaaagtc ggttgcctgc
    53881 cgggttgctg gatattggcg tcagcaaacg gcaaatcgcc gtcatcagta aaacggacat
    53941 gccggatgct gacgtcgccg caacgcgaaa gttattgctt gagacgggct tcgaggagcc
    54001 gattttcgag ctaaacagcc acgacccgca aagcgtgcag caactggtgg attatctggc
    54061 atctctcacc aaacaggagg aagcaggtga aaaaactcat cacagcgaat gatattcgtg
    54121 aagcacacgc gcgcggcgaa caggcaatgt ccgtggttct gcgcgccagc attattaccc
    54181 cggaagcccg tgaagtggcg gatctactgg gttttaccat caccgaatgt gatgaattga
    54241 tcccggtaac ggcgtccgtg cccgccagcg tgccagccga taaaaccgaa agccagcgca
    54301 tccgcgaaac cattatcgcc cagctaccgg aaggccagtt taccgaaagc ctggtcgcgc
    54361 agctgatgga aaaagtgatg aaggaaaaac agtcgctgga gcagggtgca atgcagccga
    54421 gcttcaaatc ggtgaccggc aaaggcggca tcaaagtgat tgatggtagc agcgtcaagt
    54481 ttggtcgctt tgatggcgca caaccgcatt gcgtgggctt gactgatctt gtgaccggag
    54541 acgacggcag cagcatggcc gccggtttta tgcagtggga gaacgcattc ttcccgtgga
    54601 ccctgaacta cgacgaaatc gacatggtgc tggaagggga gctccatgtt cgccacgaag
    54661 gcgaaaccat gatcgctaaa gcaggcgacg tgatgtttat cccgaaaggc tccagcatcg
    54721 aatttggtac gacatccagc gtgaaattcc tgtatgtcgc ctggccggct aactggcaat
    54781 ccctatgaaa gatttcatca ccgaagcatg gctaagagcg aaccatacgc tcagcgaagg
    54841 cgcagagatc catctccctg cggacagtcg cctgacgccc tctgcccggg agttgctgga
    54901 aagccgccat ctgcgcatca agtttattga cgagcagggc cgcctgtttg ttgacgatga
    54961 acagcagcag ccgcagcccg ttcatgggct aaccagtagc gatgaacatc cgcaggcgtg
    55021 ctgcgaactg tgccgccagc cggtggcaaa aaagccggat acgctgactc acctgtcggc
    55081 ggaaaaaatg gtcgccaaaa gcgatccgcg tctgggtttt cgcgcagttc tcgacagcac
    55141 cattgcgctg gcagtgtggc tgcaaattga actggcggaa ccgctgggct gccaggcgat
    55201 tgtcgggctt tctgacgaag atctgcaccg gctttctcat cagccgttgc gttatcttgg
    55261 tcacgatcat ctggtgccgg aagcaagcca cgggcgcgat gcggcgttgc tgaatctgct
    55321 acgcaccaaa gtgcgggaag ccgaaaccgt agcggcgcag gtgtttatca cccgcagttt
    55381 tgaagtatta cgcccggaca tcttgcaggc gctgaaccgc ctttccagca cggtctacgt
    55441 gatgatgatc ctgagcgtaa cgaagcagcc gctaacggtg aagcagatcc aacaacgact
    55501 gggggaaacg caatgattat tgaacgttgt tgtgaactgg cgttgcgagc gcccgccaga
    55561 gtggtttttc cggatgcgtt agatcaacgt gtgctgaaag ctgcgcaata tttacatcaa
    55621 caaggtctgg caacgcccat tctggtcgcc aatccgtttg aacttcgtca gtttgcgctc
    55681 agtcacggcg tggcgatgga cgggctacag gtgatagatc cgcatggcaa cctcgcaatg
    55741 cgggaagaat ttgctcatcg ctggctggcc cgcgcgggcg aaaaaacgcc gccggatgcg
    55801 ctggaaaaac ttaccgatcc gctgatgttc gccgccgcaa tggtcagcgc cggtaaagcg
    55861 gatgtctgta tcgcgggcaa cctctcttcc acggcgaatg tgctgcgtgc cggattacgc
    55921 attatcggct tgcagccagg ctgtaaaacg ctctcatcca ttttcctgat gctgccacag
    55981 tacagcggtc cggcgttggg ctttgccgat tgcagcgtgg taccacagcc gacggcggcg
    56041 cagctggcgg atatcgcgct tgccagcgcc gaaacctggc gcgccatcac cggagaagag
    56101 ccgcgcgtgg cgatgctgtc gttttccagt aacggtagcg cccgtcaccc ctgcgttgcc
    56161 aatgtccagc aggcgacaga aatcgtccgt gagcgcgcac caaagctggt agtcgatggc
    56221 gagttgcagt ttgacgccgc cttcgtgccg gaagtggcgg cgcaaaaagc gcctgccagc
    56281 ccgctacagg gcaaggccaa tgtgatggtt tttccgtcgc tggaagccgg aaatattggc
    56341 tacaaaatcg cacaacgact cggcggatat cgtgccgtcg ggccattgat acaaggactt
    56401 gccgcgccga tgcacgatct ctctcgtggt tgtagtgtgc aggaaattat cgagctggcg
    56461 ctggtggcag ctgtgccgcg tcagacagaa gtgaaccgcg aaagcagttt acaaacactg
    56521 gttgaatgaa cggtcccgtt ctggaccctg ataaagagga aagcacaatg gaagcattag
    56581 gaatgatcga aacccggggc ctggttgcac tgattgaggc ttctgacgcg atggtgaaag
    56641 ctgcgcgcgt caagctggtt ggtgttaagc agattggcgg cggcctgtgt actgccatgg
    56701 ttcgtggtga tgttgcagcc tgtaaagcgg cgaccgatgc cggtgccgca gcggcacagc
    56761 gtatcggcga gctggtatct gtacacgtta tcccgcgtcc gcacggcgac ctggaagaag
    56821 tgttcccgat cggcctgaaa ggcgatagca gcaacctgta atcaccctgt gtgacgtcga
    56881 ttcgggcgtc acgctctctc atttttcatt ttaccttccg cggagggtag ggggagcttt
    56941 tacctgaaat acgccacgga ggcgggtatg aaactggcag tcgtcactgg acaaattgtt
    57001 tgtaccgtac gccatcacgg actggcgcat gacaaattgc tgatggtgga aatgattgat
    57061 ccacatggta atcctgacgg gcaatgtgcc gtcgccatcg acaatattgg cgcgggaacc
    57121 ggggagtggg tgttgctggt gagtggcagt tccgcccgcc aggcgcataa aagcgaaacg
    57181 tcaccggtcg atctgtgcgt gattggcatc gtcgatgagg tggtgtctgg cggtcaggta
    57241 attttccaca aataaggcag aacatcatga atcaacagga tattgaacag gtggtgaaag
    57301 cggtactgct gaaaatgcaa agcagtgaca cgccgcccgc cgccgttcat gagatgggcg
    57361 ttttcgcgtc cctggatgac gctgttgcgg cagccaaaat tgcccagcaa gggttaaaga
    57421 gcgtggcaat gcgccagtta gccattgctg ccattcgtga agcaggcgaa aaacacgcca
    57481 gagatttagc ggaacttgcc gtcagtgaaa ccggcatggg tcgcgttgaa gataaatttg
    57541 ccaaaaacgt cgctcaggca cgcggcacgc caggcgttga gtgtctctct ccgcaagtgc
    57601 tgacgggcga caacggcctg actttaattg aaaacgcgcc ctggggcgtg gtggcttcgg
    57661 tgacgccgtc cactaacccg gcggcaaccg taattaataa cgccatcagc ctgattgccg
    57721 cgggcaacag cgtcattttt gccccgcatc cggcggcgaa aaaagtctcc cagcgggcga
    57781 tcacgctgct caaccaggct attgttgccg caggcgggcc ggaaaactta ctggttactg
    57841 tggcaaatcc ggatatcgaa accgcgcaac gcttgttcaa gtttccgggt atcggcctgc
    57901 tggtggtgac cggcggcgaa gcggtagtag aagcggcgcg taaacacacc aataaacgtc
    57961 tgattgccgc aggcgctggc aacccgccgg tagtggtgga tgaaaccgct gacctcgccc
    58021 gtgctgctca gtccatcgtc aaaggcgcgt ctttcgataa caacatcatt tgtgccgacg
    58081 aaaaggtact gattgttgtt gatagcgtag ccgatgaact gatgcgtctg atggaaggcc
    58141 agcacgcggt gaaactgacc gcagaacagg cgcagcagct gcaaccggtg ttgctgaaaa
    58201 atatcgacga gcgcggaaaa ggcaccgtca gccgtgactg ggttggtcgc gacgcaggca
    58261 aaatcgcggc ggcaatcggc cttaaagttc cgcaagaaac gcgcctgctg tttgtggaaa
    58321 ccaccgcaga acatccgttt gccgtgactg aactgatgat gccggtgttg cccgtcgtgc
    58381 gcgtcgccaa cgtggcggat gccattgcgc tagcggtgaa actggaaggc ggttgccacc
    58441 acacggcggc aatgcactcg cgcaacatcg aaaacatgaa ccagatggcg aatgctattg
    58501 ataccagcat tttcgttaag aacggac
//
LOCUS       DAZKFZ010000008        51692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000008 DAZKFZ010000000
VERSION     DAZKFZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..167
                     /gene="yhfL"
                     /locus_tag="ACHOQW_000468"
     CDS             <1..167
                     /gene="yhfL"
                     /locus_tag="ACHOQW_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP9571600.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
     gene            463..1800
                     /gene="frlA"
                     /locus_tag="ACHOQW_000469"
     CDS             463..1800
                     /gene="frlA"
                     /locus_tag="ACHOQW_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP9571601.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1821..2843
                     /gene="frlB"
                     /locus_tag="ACHOQW_000470"
     CDS             1821..2843
                     /gene="frlB"
                     /locus_tag="ACHOQW_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP9571602.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2894..3721
                     /gene="frlC"
                     /locus_tag="ACHOQW_000471"
     CDS             2894..3721
                     /gene="frlC"
                     /locus_tag="ACHOQW_000471"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP9571603.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYALQALERFRALLPEDE"
     gene            3721..4506
                     /gene="frlD"
                     /locus_tag="ACHOQW_000472"
                     /pseudo
     CDS             3721..4506
                     /gene="frlD"
                     /locus_tag="ACHOQW_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            4606..5337
                     /gene="frlR"
                     /locus_tag="ACHOQW_000473"
                     /gene_synonym="yhfR"
     CDS             4606..5337
                     /gene="frlR"
                     /locus_tag="ACHOQW_000473"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP9571604.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5468..6472)
                     /gene="trpS"
                     /locus_tag="ACHOQW_000474"
     CDS             complement(5468..6472)
                     /gene="trpS"
                     /locus_tag="ACHOQW_000474"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9571605.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(6465..7223)
                     /gene="gph"
                     /locus_tag="ACHOQW_000475"
     CDS             complement(6465..7223)
                     /gene="gph"
                     /locus_tag="ACHOQW_000475"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP9571606.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(7216..7893)
                     /gene="rpe"
                     /locus_tag="ACHOQW_000476"
     CDS             complement(7216..7893)
                     /gene="rpe"
                     /locus_tag="ACHOQW_000476"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9571607.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(7911..8747)
                     /gene="dam"
                     /locus_tag="ACHOQW_000477"
     CDS             complement(7911..8747)
                     /gene="dam"
                     /locus_tag="ACHOQW_000477"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9571608.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(8854..10140)
                     /gene="damX"
                     /locus_tag="ACHOQW_000478"
     CDS             complement(8854..10140)
                     /gene="damX"
                     /locus_tag="ACHOQW_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP9571609.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(10232..11320)
                     /gene="aroB"
                     /locus_tag="ACHOQW_000479"
     CDS             complement(10232..11320)
                     /gene="aroB"
                     /locus_tag="ACHOQW_000479"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9571610.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(11377..11898)
                     /gene="aroK"
                     /locus_tag="ACHOQW_000480"
     CDS             complement(11377..11898)
                     /gene="aroK"
                     /locus_tag="ACHOQW_000480"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP9571611.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(12299..13537)
                     /gene="hofQ"
                     /locus_tag="ACHOQW_000481"
     CDS             complement(12299..13537)
                     /gene="hofQ"
                     /locus_tag="ACHOQW_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP9571612.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(13449..13853)
                     /gene="hofP"
                     /locus_tag="ACHOQW_000482"
     CDS             complement(13449..13853)
                     /gene="hofP"
                     /locus_tag="ACHOQW_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP9571613.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(13843..14283)
                     /gene="hofO"
                     /locus_tag="ACHOQW_000483"
     CDS             complement(13843..14283)
                     /gene="hofO"
                     /locus_tag="ACHOQW_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP9571614.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(14267..14806)
                     /gene="hofN"
                     /locus_tag="ACHOQW_000484"
     CDS             complement(14267..14806)
                     /gene="hofN"
                     /locus_tag="ACHOQW_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP9571615.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(14790..15584)
                     /gene="hofM"
                     /locus_tag="ACHOQW_000485"
     CDS             complement(14790..15584)
                     /gene="hofM"
                     /locus_tag="ACHOQW_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP9571616.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDARRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            15704..18256
                     /gene="mrcA"
                     /locus_tag="ACHOQW_000486"
     CDS             15704..18256
                     /gene="mrcA"
                     /locus_tag="ACHOQW_000486"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP9571617.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(18421..18981)
                     /gene="nudE"
                     /locus_tag="ACHOQW_000487"
     CDS             complement(18421..18981)
                     /gene="nudE"
                     /locus_tag="ACHOQW_000487"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP9571618.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            19301..21436
                     /gene="igaA"
                     /locus_tag="ACHOQW_000488"
     CDS             19301..21436
                     /gene="igaA"
                     /locus_tag="ACHOQW_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP9571619.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21501..22169
                     /gene="yrfG"
                     /locus_tag="ACHOQW_000489"
     CDS             21501..22169
                     /gene="yrfG"
                     /locus_tag="ACHOQW_000489"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP9571620.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            22180..22581
                     /gene="hslR"
                     /locus_tag="ACHOQW_000490"
     CDS             22180..22581
                     /gene="hslR"
                     /locus_tag="ACHOQW_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP9571621.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            22606..23484
                     /gene="hslO"
                     /locus_tag="ACHOQW_000491"
     CDS             22606..23484
                     /gene="hslO"
                     /locus_tag="ACHOQW_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9571622.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23547..25271)
                     /gene="yhgE"
                     /locus_tag="ACHOQW_000492"
     CDS             complement(23547..25271)
                     /gene="yhgE"
                     /locus_tag="ACHOQW_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP9571623.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            25650..27272
                     /gene="pckA"
                     /locus_tag="ACHOQW_000493"
     CDS             25650..27272
                     /gene="pckA"
                     /locus_tag="ACHOQW_000493"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9571624.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(27348..28700)
                     /gene="envZ"
                     /locus_tag="ACHOQW_000494"
     CDS             complement(27348..28700)
                     /gene="envZ"
                     /locus_tag="ACHOQW_000494"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP9571625.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(28697..29416)
                     /gene="ompR"
                     /locus_tag="ACHOQW_000495"
     CDS             complement(28697..29416)
                     /gene="ompR"
                     /locus_tag="ACHOQW_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP9571626.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            29644..30120
                     /gene="greB"
                     /locus_tag="ACHOQW_000496"
     CDS             29644..30120
                     /gene="greB"
                     /locus_tag="ACHOQW_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP9571627.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            30217..32538
                     /gene="yhgF"
                     /locus_tag="ACHOQW_000497"
     CDS             30217..32538
                     /gene="yhgF"
                     /locus_tag="ACHOQW_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9571628.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            32976..33203
                     /gene="feoA"
                     /locus_tag="ACHOQW_000498"
     CDS             32976..33203
                     /gene="feoA"
                     /locus_tag="ACHOQW_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP9571629.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            33220..35541
                     /gene="feoB"
                     /locus_tag="ACHOQW_000499"
     CDS             33220..35541
                     /gene="feoB"
                     /locus_tag="ACHOQW_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP9571630.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            35541..35777
                     /gene="feoC"
                     /locus_tag="ACHOQW_000500"
     CDS             35541..35777
                     /gene="feoC"
                     /locus_tag="ACHOQW_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP9571631.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            35980..36858
                     /gene="rpnA"
                     /locus_tag="ACHOQW_000501"
     CDS             35980..36858
                     /gene="rpnA"
                     /locus_tag="ACHOQW_000501"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP9571632.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(36885..37655)
                     /gene="bioH"
                     /locus_tag="ACHOQW_000502"
     CDS             complement(36885..37655)
                     /gene="bioH"
                     /locus_tag="ACHOQW_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP9571633.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            37693..38376
                     /gene="gntX"
                     /locus_tag="ACHOQW_000503"
     CDS             37693..38376
                     /gene="gntX"
                     /locus_tag="ACHOQW_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP9571634.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            38435..39010
                     /gene="nfuA"
                     /locus_tag="ACHOQW_000504"
     CDS             38435..39010
                     /gene="nfuA"
                     /locus_tag="ACHOQW_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP9571635.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            39371..40687
                     /gene="gntT"
                     /locus_tag="ACHOQW_000505"
     CDS             39371..40687
                     /gene="gntT"
                     /locus_tag="ACHOQW_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9571636.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(40732..42816)
                     /gene="malQ"
                     /locus_tag="ACHOQW_000506"
     CDS             complement(40732..42816)
                     /gene="malQ"
                     /locus_tag="ACHOQW_000506"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP9571637.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(42826..45219)
                     /gene="malP"
                     /locus_tag="ACHOQW_000507"
     CDS             complement(42826..45219)
                     /gene="malP"
                     /locus_tag="ACHOQW_000507"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP9571638.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            45831..48536
                     /gene="malT"
                     /locus_tag="ACHOQW_000508"
     CDS             45831..48536
                     /gene="malT"
                     /locus_tag="ACHOQW_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP9571639.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            48603..48881
                     /locus_tag="ACHOQW_000509"
     CDS             48603..48881
                     /locus_tag="ACHOQW_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9571640.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            48872..49357
                     /locus_tag="ACHOQW_000510"
     CDS             48872..49357
                     /locus_tag="ACHOQW_000510"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9571641.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(49407..50435)
                     /gene="rtcA"
                     /locus_tag="ACHOQW_000511"
     CDS             complement(49407..50435)
                     /gene="rtcA"
                     /locus_tag="ACHOQW_000511"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP9571642.1"
                     /translation="MKRMIALDGAQGEGGGQIMRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(50439..51665)
                     /gene="rtcB"
                     /locus_tag="ACHOQW_000512"
     CDS             complement(50439..51665)
                     /gene="rtcB"
                     /locus_tag="ACHOQW_000512"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP9571643.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        12231 a        14115 c        13134 g        12212 t
ORIGIN      
        1 tgaacaaatt tattaaagtt gcactggtag gtgcagtact ggctacgtta actgcatgta
       61 ctggtcatat tgaaaaccgt gataagaact gctcttacga ctacctgctg cacccagcaa
      121 tttctatttc taaaatcatt ggcggttgcg gtcctactgc acagtaagtc tctgacagaa
      181 atcagctaac gctgatttaa ttgcctgacg ccctcgggat tacccgaggg cgtatttgtt
      241 tctatatcct tcttgctccc cacccgtaaa aatattttca ctttaatttc aattggataa
      301 ccacattcat cagccacagc gcgatcctgt gcgaaattat gtgatcttcc tccacattac
      361 ataacatcat atgttgttat attcatcatg cattgtcatg tcacctttta aatgactgca
      421 aactctcccc tacaacgtgt tggacaagaa aaaggtatcg ctatgggaag ccaggaactc
      481 caacgcaagc tcggattttg ggccgttctt gcaatcgccg tcgggacaac cgtcggctcc
      541 ggtatttttg tatctgtggg tgaagtggca aaagcggcgg gcacgccgtg gctgacggtg
      601 ctcgcatttg tcatcggcgg gttaattgtg atcccgcaaa tgtgcgtata tgcggaacta
      661 tccaccgctt atccggaaaa tggcgcagat tatgtttatc tgaaaaatgc cggaagccga
      721 ccgctggctt tcctctccgg ctgggccagc ttctgggcca acgatgcgcc gtcattgtcg
      781 attatggcgc tggcgattgt cagcaatctt ggctttttaa cgcctatcga tccgttgctc
      841 ggtaaattta tcgccgccgg attaattatc gcctttatgc tgctgcacct gcgctccgtt
      901 gaaggcggcg cagcgtttca gacgctaatt accatcgcca aaattatccc gttcactatc
      961 gtcattggtc ttgggatctt ctggtttaaa gcggagaatt ttgccgcccc tgctaccact
     1021 gcgattggcg caacgggcag ctttatggcg ctgctggcgg ggatctctgc caccagttgg
     1081 tcgtataccg gcatggcctc tatctgttat atgaccggcg aaattaaaaa ccccggcaaa
     1141 accatgccac gagcgctgat tggttcctgt ctgctggttc tggtgctcta caccctgctg
     1201 gcgctggtga tttccggcct gatgcccttc gacaaactcg ccaattctga aacgccgatt
     1261 tccgacgccc tgacctggat ccccgcactc ggcagcaccg ctgggatctt tgttgccatc
     1321 acggcgatga tcgtcattct tggttcgctt tccagctgcg tgatgtacca gccgcggctg
     1381 gaatacgcga tggcgaaaga caacctgttc ttcaaatgct tcggccatgt gcatccgaaa
     1441 tacaacacgc cggatgtctc catcattttg caaggggcgc tggggatctt cttcatcttc
     1501 gtttccgatc tcaccagcct gctgggctat ttcaccctgg taatgtgttt caaaaatacc
     1561 ctcaccttcg gctccatcat ctggtgtcgt aaacgcgacg attacaaacc gctgtggcgt
     1621 actccggctt tcgggctgat gacccccctc gccattgcgt caagcctcat tctggtcgcc
     1681 tcaacctttg tctgggcacc gattcccggc cttatctgcg ccgtcatcgt tattgccact
     1741 ggtctgcctg cttacgcctt ctgggcgaag cgtagccgcc agctcaacgc tttgtcgtaa
     1801 tgttatctgg agaaaataaa atgttggata ttgataaaag cactgtggac tttctggtca
     1861 ccgaaaatat ggttcaggaa gtggaaaaag ttctcagcca tgacgttccg ctggtgcaca
     1921 ccatcgtgga agagatggtg aagcgcgaca tcgatcgtat ttatttcgtt gcctgcggat
     1981 cgccactcaa cgcggcgcaa acggcgaaac atctggcaga tcgcttttcc gatcttcagg
     2041 tttacgccat ttccggctgg gagttctgcg ataacacccc atatcgcctc gacgctcgtt
     2101 gcgcggtgat tggcgtttct gactacggta aaaccgaaga ggtaatcaaa gcgctggagc
     2161 tgggccgggc ctgcggcgca ctcactgcgg cgttcaccaa acgcgcggat agcccgatta
     2221 cctcggcggc ggaatttagc attgattatc aggccgactg tatctgggaa attcatctgc
     2281 tgctctgcta cagcgtggtg ctggagatga tcacccgcct cgcgccgcac gcggaaatcg
     2341 gcaagatcaa aaacgatctc aaacagctgc cgaatgcgct cggttatctg gtacgcactt
     2401 gggaagaaaa aggccgccag cttggtgaac tggccagcca gtggccgatg atttacacgg
     2461 ttgctgcagg cccgctgcgc ccgctgggtt acaaagaagg catcgtgacg ctgatggaat
     2521 ttacctggac gcacggctgt gtgattgaga gcggtgagtt ccgtcacggc ccgctggaga
     2581 ttgtcgaacc gggcgttccg ttcctgttcc tgctcggcaa tgatgaaagt cgccacacca
     2641 ccgaacgcgc cattaacttt gttaaacagc gtactgacaa cgtgatcgtc atcgattacg
     2701 ccgcaatttc gcaaggactg cacccgtggc tggcaccgtt cctgatgttc gtgccaatgg
     2761 agtggctctg ctactacctg tctatttaca aagatcacaa cccggatgaa cgccgctatt
     2821 acggtggtct ggtggaatat taatcctctc tcccggcccg gcaacgggcc ggatttacgc
     2881 aaggagttac ccgatgaaaa caggtatgtt tacctgcggc caccagcggc tgcctattga
     2941 acatgcattc cgtgatgcaa gcgagctggg ttacgatggc atcgaaattt ggggcggccg
     3001 cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca aggcgctggc
     3061 gcagacgtat cagatgccga ttatcggtta cacgccagaa accaacggct atccgtataa
     3121 catgatgctg ggcgatgaac atatgcgtcg tgaaagcctg gacatgatca agctggcgat
     3181 ggatatggca aaagagatga acgcgggtta tacgctgatt tctgcggccc acgcgggcta
     3241 tctcacgccg cctaacgtta tctgggggcg gctggcagag aacttaagcg aactgtgtga
     3301 gtacgcggaa aacatcggca tggatctgat cctcgaaccg ttaacgccgt atgaatcgaa
     3361 cgtcgtgtgt aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt cgccgcgcct
     3421 gttcagcatg gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga tgagttattt
     3481 cgacaaactg ggcgataaat tacgtcatct gcatattgtc gacagcgacg gggccagcga
     3541 cacgcattac attcccggcg aaggcaaaat gccgctgcgg gaactgatgc gcgatattat
     3601 tgatcggggc tatgagggat actgcacagt ggaactggta acgatgtata tgaacgagcc
     3661 cagactttac gccctccagg cgctggaacg ctttcgcgcg ctgctgccgg aggatgagtg
     3721 atgaaaaccc tggcgacaat cggcgataac tgcgtcgata tctacccgca actgaataaa
     3781 gcgttttctg gcggtaatgc ggtgaacgtg gcggtgtact gcactcgcta cggtataaag
     3841 ccgggatgca ttacctgggt tggtgacgat gactacggca caaagcttaa gcaggatctc
     3901 gccagcatgg gcgtcgatat cagccatgtc catacgaaac acggcgttac cgcacaaact
     3961 caggtggaac tgcacgacaa tgatcgcgtt tttggcgact acaccgaagg cgtgatggcc
     4021 gactttgccc tgagtgaaga ggattacgcc tagctggcgc agtatgacat tgtgcacgcg
     4081 gcaatctggg gacatgcgga agacgcattc ccacagctgc acgctgcggg caaacttacc
     4141 gctttcgacg tctccgacaa gtgggacagc ccgctctggc agacactggt gccgcatctc
     4201 gattttgcct ttgcctccgc accgcaagaa gacgaagcgc tgcgtctgaa gatgaaagcg
     4261 attgttgccc gtggcgcagg cgtggtgatt gtcacgttgg gtgaaaacgg cagtattgcc
     4321 tgggatggcg tgcagttctg gcgtcaggct cctgaacagg tgacggttat cgacaccatg
     4381 ggtgccggag attcgttcat tgccggattc ctttgcggct ggtctgcggg gatgacatta
     4441 ccgcaggcga tggcgcaggg aacggcgtgc gcggcacaaa ctattcagta ccacggtgcc
     4501 tggtaggcat aacgttggcg tgagcatctt cacgccaacg tgctgttact tgccggaaaa
     4561 cgaccctata atccgtgtaa ttcattcttt atttcagggt cgattatgtc agctacggac
     4621 cgctactctc atcaactcct ctacgctacc gtccgccagc ggctgctgga tgatatcgcg
     4681 cagggggttt accaggccgg gcaacagatc cctaccgaaa atgagctttg tacacaatat
     4741 aacgtcagcc gcattaccat tcgcaaagcc atcagcgact tagtggcaga cggcgtactg
     4801 atccgctggc agggcaaagg cacctttgta caaagccaga aggtcgaaaa cgccctgctg
     4861 accgtcagtg gttttaccga ttttggcgtc tcacaaggca aggcgacgaa agagaaagtg
     4921 atcgagcaag aacggatcag cgccgcgccg ttttgcgaaa agctcaatat ccccggtaac
     4981 agcgaagtgt tccatctttg ccgggtgatg tatctcgata aagagccgct atttattgat
     5041 agttcgtgga tcccgctgtc gcgttatcct gactttgatg agatttacgt cgaaggaagc
     5101 tccacctatc agttatttca ggagcgtttt gacacgcgag tggtcagcga caaaaagacc
     5161 atcgatatct ttgccgccac ccgcccgcag gcaaaatggc tgaaatgcga actgggcgaa
     5221 ccgttgtttc gcatcagcaa aatcgccttt gatcagaatg acaaaccggt gcacgtctcc
     5281 gaactcttct gccgcgccaa ccgcatcacc ttaacaattg ataataaaag acattaaccg
     5341 taggccggat aagatgcacc agcatcgcat ccggcgatgc cggcgcactg aattttacat
     5401 cccgcacgtt cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccgagcgt
     5461 tttgatatta cggcttcgcc acaaaaccaa tcgcttcgta caccgctttt agcgtacggg
     5521 aagcgtgcgc gctggctttt tctgcgccgt ctttcatcac ctgttgcagg aaggcttcat
     5581 cgttgcggaa acggtggtaa cgttcctgca attcagtcag cataccggaa acggcatcag
     5641 ccacttcgcc tttcagatga ccatacatct tgccttcgaa ctgtttttcc agttccggga
     5701 tgctctggcc cgttaccgca gaaaggatat ccagcaggtt ggaaacgccc gctttgttct
     5761 gcacatcgta gcgaactacc ggcggctcgt cggagtcggt gaccgcgcgt ttgattttct
     5821 tcactaccga tttcggatct tccagcaggc caataacgtt attgcggtta tcgtcagact
     5881 tggacatctt cttggtcggc tccagcagcg acattacgcg cgcgccggat ttcggaataa
     5941 acggctccgg caccttaaag atatcgccgt acagcgcgtt gaaacgctgg gcgatatcac
     6001 ggctcagttc caggtgctgt ttctggtctt cacccaccgg taccagatta gtttgataca
     6061 gcaggatgtc cgctgccatc agcaccggat agtcaaacag accagcgttg atgttctcgg
     6121 cataacgcgc agatttatct ttaaactgcg tcatgcggct cagttcgccg aagtaggtat
     6181 agcagttcag tgcccagcct aactgcgcat gttccggtac gtgggactga acaaaaatgg
     6241 tgcttttctc aggatcgata ccacaagcca gatacaaggc cagcgtatcc agcgtcgctt
     6301 tacgcagctt ctgtgcatcc tggcgcacgg tgatcgcgtg ttggtcaacg atacagtaaa
     6361 tgcaatggta gtcatcctgc atgtttaccc actgacgcag cgcacccatg tagttaccaa
     6421 tagtcaattc acctgagggc tgtgcgccac taaaaacgat gggcttagtc atttttcgat
     6481 tcctgatttt cgctatgcgg aagccctaat gcgggcagaa ggtcatttat agactgataa
     6541 attacatcag gctggctgag atcgatagcc tcgccgtagt tatatccgta ggttaagcca
     6601 actgatgggc aacctgccgc ttttgctgcc tgaatatcat tgcgtgagtc gccaacaaac
     6661 agcatctgtt gtggagcaat tcccatccgc tcagccacca gtaacagcgg gtccggatgc
     6721 ggttttttgt tttgcacatc atcaccacca atcaccacgc tgaagtattt ggcgatatct
     6781 aaggcttcga gcagcggcgc gacgaacggc gtcggtttgt tggtgaccag gcctagcggc
     6841 aggcctttag cctgcaacgc gcccaacgta tcggcaacgt gcgggaacaa aaacgtcccc
     6901 tcttcggcaa cctcgccata gtaacgatcg aacagtttac gcagaatacg tacctgttct
     6961 tctgccggaa tgtcgtcatc aacgggcggt ttacccattg ttttacgcag agtcgcacgt
     7021 tcctgacgcg cccaggtcaa tgcgcgctcc atcagaacat ctgcgccgtt accaatccag
     7081 gtaataacgc gttcttcacc tgcgacgggc aactccagcg catacagcgc catatctacc
     7141 gcagcagcaa gaccgggagc actgtccacc agcgtaccgt cgagatcaaa agcgacgccg
     7201 cgaatatctt caaacttact catgacttac ctttgccagt tcactgcgca tttcatcaat
     7261 gacttttttg tagtctggct ggtcgaagat tgccgaaccg gcaacgaaca tatccgcgcc
     7321 cgccgcggcg atttcgccaa tgttgttcac cttcacgcca ccgtccactt ccagacgaat
     7381 gtcaaagcca gactcgtcga tacgacgacg tacttcgcgc agtttatcca gtgtttgagg
     7441 aatgaaagat tgaccgccga aaccagggtt gacggacatc agcaggatca catccagctt
     7501 atccatcacg taatccagat agctcagagg tgtcgccggg ttaaatacca gacccgcttt
     7561 acagccattt tctttaatca gttgcagcgt gcggtcaaca tgctcggagg cttctggatg
     7621 aaaggtaatg atgctggcac cagcggcagc gaaatcaggc acaatgcgat cgaccggttt
     7681 caccatcagg tgtacgtcga taggggcggt aatgccgtag ttacgcaagg atttcagcac
     7741 cattggccca atcgtcagat tgggaacgta gtggttatcc atgacgtcaa aatgcacgac
     7801 atcagcgcca gctgccaggg cttttgcggt atcttcaccc aggcgggcaa aatcagccga
     7861 cagaattgag ggggcaatca aatactgttt catccgcttc tccttgagaa ttattttttc
     7921 gcgggtgaaa cgactcctgg tttgtacaaa gccagcagtt cgtccacctt tttacgtgtg
     7981 ccgccgttgc tgcttatact gcgtcgaact ttgacgacat gcaattttgc gcgctgatac
     8041 cactcacgcg ttaacatcgt atcgtgattg gagatcagca caggaatatg gcgctcaacc
     8101 agaccttcgg cgatctccgc cagatgcgct tgttgttcaa gcgtaaaact gtttgtgtga
     8161 tacgccgtaa agttggcggt cgcagacagc ggtgcataag gtggatcgca atagacgaca
     8221 gatgcatcat ctgcgcgcgc catgctatcg gcgtaagact cacaatagaa aaaggcattc
     8281 tgcgcttttt cagcgaagtg atacaactct gcttccggga aatagggttt tttgtagcgg
     8341 ccaaacggca cattaaactc accgcgcaga ttgtaacgac acaggccgtt gtaaccgtag
     8401 cggttcaaat ataaaaacag taccgcccga cggaacggat cctggctttt gttgaactct
     8461 tcgcggaact gatagtaaac ctcggcgcaa tttgtttcgg gaacaaacag ctcgcgtgcg
     8521 gcctgtacgt actcatcagt acgcatcttc acaatgttat agagactgat caggtcgcta
     8581 ttgatatcgg caaggatata acgagaaaag tcggtgttga gaaacaccga cccggcacct
     8641 acaaaaggct caaccagaca ttcgcccttg ggcaaatgcc gtttaatatc atcaagcagg
     8701 ggatacttgc cccctgccca cttcaaaaaa gcgcgatttt tcttcatgct gactaactaa
     8761 ttacaccttc tccggctgtg gagaaagctc cgacagcatc cagcgcttca ggcaactccc
     8821 gcaacgataa accattgcgg gagataacct tgattacttc agatcggcct gtacctgacg
     8881 cagcggtttc gcccacgggt ttttggcctg aacatctgct ggcaatgtag ataccgcttt
     8941 tttcgcctct tctttcgaag cgtacacgcc agaaaccagg acataccacg gctgaccatt
     9001 acgcgtcgtt tcatagacaa cgtagttttt cagattctct ttcttcgccc aaccgttcag
     9061 gttgtcgtag ttagaggaac tgctcagctg cagagtgtaa tggctggacg gtgccgattt
     9121 caacgaaccc acattacctg cggtcttccc tccagcggct ggtgttgccg tggtttgcgc
     9181 cggggatgcc gtctgtactg gagccgtagt cgccgtctct tttggtgctg gcgtagaagt
     9241 cgcagccggt gccttcgtgc tcgccacagg agcagctggt tcagtacgct tcggcgtctg
     9301 tgctaccggc ttcggttccg ttttcacggt tgcttgcggt tttttcggtt caatcaccgc
     9361 ctgctgacga gctggacgcg tagtggccgg acgttcagca gtttgcgttt tcgccgtgtc
     9421 acgcgatgca ttgccattgc gtactggcgc gacagttgct ggttcagtcg gcaatgtaga
     9481 attgaccgcc acattgttca actgttgctg attttgtggc tgggtcagcg cattgttcag
     9541 gtcaccctgc acttcaacac gttgttgacc atccgttgcc gccggggttt gcccttgagt
     9601 cggcgtagaa gagatcggtg gcagagaaac atcctgctga gtattttccg cagacgtggt
     9661 tcccggcgct ggctgcacgc cattcgcctg atcggtcgca ttgccagcaa gatcaatact
     9721 cttctcgcca gacgcggttt gatcgctgga agtggtcgag ggggctttta gcgcagaacc
     9781 gataccgatg atcaacagca gtagaaccag aatgccgacg cccatcatca tatactgacg
     9841 agaagcgggt ttactggctg cttttttgcg cttacgcgga cgacgctcta cgcgctcttc
     9901 atccacggtt tcatcttcgg attcgtcaat ttcttcttcg atttccggtt cctcattgcg
     9961 ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca tcaagttcaa tatcatcaaa
    10021 attgatctgc ggttcgccac gttcagtacg ctcagaacgt tcagaagatt gacgagaacg
    10081 accagtacga cgatcgctgg gatcgggttt cagctcgtct tctggtttga attcatccat
    10141 ttaacacccc actaaaaggt taatgcttac cacgttgcaa ttaacctgaa gctaatagac
    10201 cgcttgataa gcggcctgac ctttcttgtt gttacgctga ttgacaatcg gcaatggcgt
    10261 taagaacaag ctcgtgcgaa acgccgctgc gaacttcact cttaccaatt gccaacggaa
    10321 gaattaagcg catctctccc gcaaggactt tcttgtcacg cagcatatgc ggtaaatacg
    10381 cctgcgcgga catttcgcgc ggcccattga ccggtaaccc agcccgcgtg agcagagtta
    10441 taatacgctg cgtttcggca gaactaaact gcccgagacg ttccgacgtc cgcgccgcca
    10501 tcaccatacc cgcagcgacc gcttcaccat gtaaccaatt gccatacccc atttcagctt
    10561 caatggcatg accaaaggtg tgtcccagat tcagtaaagc acgtaacccg gtttcgcgct
    10621 cgtcggcggc gacaacttct gccttcagtt cacaacaacg gcgaatacag tacgccattg
    10681 ccggaccgtc cagacgcaac aacgcatcca gattctcttc cagccagtta aaaaacgtac
    10741 cgtcaagaat aatgccgtat ttgatgactt ctgccagccc cgacgctaac tcacgcgggg
    10801 gaagcgtttt cagacagtcg agatccacca ccactgaagc aggctggtag aacgcgccaa
    10861 tcatgttttt accgagggga tggttgaccg cagttttgcc gccaacggag gaatcgacct
    10921 gcgacagtaa cgtcgtcggg acttgaatga aacgaacacc gcgctgataa ctcgccgccg
    10981 cgaagccggt cagatcgccc actacgccgc cgccaagcgc caccagcgta gtatcgcgac
    11041 catgcggctt ttgtaacaac gccgtaaaga cggtatcgag tacagccagg cttttatact
    11101 gctcgccgtc aggaaggata acgctatcga cgttaacacc cgcctgttca agtacgccgc
    11161 ggaccttatc gagatacaga ggagccaggg tttcgttggt gaccaacatg acctgctcgc
    11221 ccgatttcag cggtaagaat gaagctggtt cattaaacaa accagatgcg atggtaattg
    11281 ggtaactacg ttccccgaga gtaacgacaa tcctctccat aacgcgacat ccaccttaat
    11341 tactgtaccc gcagacgagt gtatataaag ccagaattag ttgctttcca gcatgtgaat
    11401 aatctggttt gcaaccactt tagcgctttg atcatcagta cgaatggtca cgtcggcaat
    11461 ctcttcatac agcggattgc gttcattggc caacgcttcc agaacttcac gcggcggtgt
    11521 ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg cgtgcaagtt gcttttcgat
    11581 cgtcgtttca agataaacga caacgccacg agcggaaaga cggttacgcg tttcacggga
    11641 tttcacagag ccgccgccag tagccagcac aataccctgt ttctcggtca actcattgat
    11701 gaccttttct tcgcgatcgc ggaagccttc ttcgccttct aaatcgaaaa cccagcccac
    11761 atcagctccg gttcgtttct caatctcttg atcggaatcg taaaattcca tattgagttg
    11821 ttgagctaac tggcgcccaa tagtgctttt tccggcaccc ataggcccaa ccagaaagat
    11881 attgcgtttc tctgccattt tttcggtact actaagacta ttcgttaatg ataaacccgc
    11941 ttcgctcaga gagctccgca ggacatgaac tgaaacctca taagatattg cgagagtcag
    12001 actgaaaatt atctcaatac tccagcgggt ttggcaactg aataaatcac ccatccctct
    12061 gcatatctgg tcgctgcaag cgcgctgcct tgctaccacc gctctggcga taaatcaccg
    12121 ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga atgataaatc atcatcatgg
    12181 aaggcaggca gcgaagagca ccaactctca aatcggtact ccttgtatgc taaatacctg
    12241 cgcgcgtcaa atagatgaaa cacgttcagc gtaaaaacat taccgctttt acggctgttt
    12301 actcactgga aaccagtcgt ggcgtgataa acaccactaa ctcgcgtcgt tcatcttctt
    12361 ttccgtcatg acgaaataat tgcccgaacc agggaatgtc gccaagcaac ggcacgctat
    12421 cctgacccga tttattttta cgggtaaaaa tgccgcccag cgccaacgtt tctccgcttt
    12481 tgacctcgac ctgcgtttcg atctcctgct tatcaatcgc cagcacttcg ccatcggcct
    12541 gctgtagcac ctgccccgga acgttctggc tgatgtgtaa tttcagccgg atgcgacctt
    12601 tttgtaacac cgtgggcgtg acctccatcc ccaggacggc ctctttaaat tccaccgacg
    12661 tcgcgccact ttccccgctg gaaacctgat atggaatttc gctcccctgt ttaatgctgg
    12721 caggctgaag atgtgaggcc agcagacgcg gactggcgat aatatccagc tgctgttttt
    12781 gttcgagcgc ggaaagctca agatccagca aacgtccgtt gatgcgccca atgttaaaac
    12841 cgacatgcgt tgtcgccgtc gctacggaga ggtcgctacc aagcgtggtg acttgcccaa
    12901 cgccaccagc gtgttgcgca tcggccagcg tccatttcac gcctaactca cgcaaacttt
    12961 tttcattaat ggtgacaata tgcgccgaca gctcaacctg cccgaccggc agatccattt
    13021 gcgctaccca ctgttcaagg gcgcttaacg ccgttttgtt atctcgtagc aaaaggcgat
    13081 tggtgcgttt atcgacggtc atactccctt tggcactcag tagcttctcc ccagctttcg
    13141 ccagctctcc ggcgtcggcg tattgcagag taatattgcg attttccagc ggcagatttg
    13201 cctgcgcccg cgcctgctcc gcttcctgac gggcgatatt gtcattctgc caggcaatgg
    13261 aatgcactga gagaatgttg ccctcctggc gcgttatcag tccggcgctt ttcactacag
    13321 tttgtagtgc ctgcttccag ggaacatctg ttagatgtaa cgacaccgta ccgctgacgt
    13381 ctggcgacac caccaggttc aacttctcct gttcagccag cgcctgcaac acctgagcta
    13441 ccggaacgtc atccaccatc agcgtcactt tttgcggctt tgccgcctgt acgccgggta
    13501 tcagcatcaa cagtagtgcg gctatccatt gcttcatttg tatctccttg ccgttgccac
    13561 aaccattgtg gcggttcgca gtttgtcccg gtacccagcg ttagtacgtc tggcgtcagc
    13621 tgtaaaattg tccagccgtt ttccagcaca tcgttttgct gcacccgtcg ccatttcttt
    13681 tgcccgtctt ttattacacc gatgatgcgc tcgcctcgcc ctaccatccc ctgatagcgc
    13741 cactggctaa gttcgctaat ccggcataga tcttccggcg gtttaaaagg gtcacgcata
    13801 ccggttaaaa ggcacaatgc aatacctgcc aacaaccagc gtttaaccct catgcggcgt
    13861 ctccagttgt agggtgaaca aaagatcatc accttccacg cttaatgaaa aacggctcac
    13921 gctgacgttg cgctctgcca gccgtgtaaa tgccgacggc actgcttccc acagcgtttt
    13981 caacgccaac tcgcctccct gcgcggatgg atgccagaaa accagttgtg cgccgggcaa
    14041 ctgaaaatcc agtggcgaaa agggcagcgt tttttcttcg ctgaagggag tggtgtcaac
    14101 caggcgatac agtgtggccc aatgctgatg atgacttgcc cgtagtcgaa ttaatgcgtc
    14161 gcgctcttca tggtgtgtcg atgacagaaa aatgagcgta actaacatca gcaaccagaa
    14221 tgcccagcaa aactggcgga ggcggggtga tgtggcgaac caccagtcaa agaacatgtt
    14281 catcgctaac cttccttgtt aactgatact caaattgcca gcgtccctgc gcatcctgct
    14341 gcgtggcccc ccgctgattg agatgaaaag atgcatcctg gcggagtgac gtttctagtg
    14401 cgtttaacgc ggtaatgctt gttgtaagcc ccttgatctc cagcgttccc tgctgccagc
    14461 ttatcgttgt cagccaggcg tgctcgggta aaagcgccgc cagtgcttcc agcgcagatt
    14521 gccagtcgcg ggtaaattgt cgctggcgct gcctttgcaa acgctgctca cgaatttgtt
    14581 ggcgctccag caaacgtggt ttggttatct gtaagctgcg ggcgagttgt tgttccccct
    14641 gaagcaaaac ggcgtctacg cgagcttcgg cgctgcttgt cagacgcagt attagcgtta
    14701 tcccgacggc cagcagcaga ggcgcaacga acatcagcaa ccagaaacga agaaaagcgg
    14761 tccggcgttg ctgtcgccag ggcaaaaaat taattggcgg gttcatcagt acgccttccc
    14821 aagcgccagc ccagcgcgat ggcaaagtct ccacctggcg gcggtaacgg cggctgacga
    14881 acagaaacgg cctcccaagg atcaaatccg ccttcgccac atatcgcgac gctttcggga
    14941 tcaacggata acgctgccgc cagctccttc gcgctagtca tccccaccgc cagtttgcgc
    15001 ccccagcgat agcgcgtcgc ccacagccac tgttcgttat cacgccaggc cagacattgc
    15061 tgatgagaag gtaaaaaagg caggaatcgc tgtaatgcac tggcatccgg ggtgatcgca
    15121 ctaacatgaa cacgcaacct ttctgccaaa gtaagcagcg ttgccagctc tttgctttgc
    15181 gcggcagtca cgttataggc gggactgagt gagtcttcgc tgtaatcgaa gcgcagggag
    15241 tccggatcca tatccagctc gcgagccatc gtcccggaca gccaggccgt ttgctcccgc
    15301 tcaccgaggg acatcgacgg gcgcggaaat gaccgctgta atgtgcgact ggcgggaaac
    15361 gccaacatga tgtgatggcg ctgcggcagt tcgcgactcc acggtaacaa cgttttagcc
    15421 agccgccgcg catcaacaat ccgcccatct ttgataatgt cgtgcgccag cggcaaccgc
    15481 caccagcgtt gcaaaaagca ttcttttgcg ccccgcacaa tagcaaccgc taccgcttct
    15541 tgctgttgta aatgcaaacc aatttgccag atcttaaatg ccattgtgat gatctcctta
    15601 tcacccgtca ctctgacggg tatatcaatg cgtctggctt gcctttatac taccgcgcgt
    15661 ttgtttataa actgcccaaa tgaaactaaa tgggaaattt ccagtgaagt tcgtaaagta
    15721 ttttttgatc cttgcagtct gttgcattct gctgggagca ggctcgattt atggcctata
    15781 ccgctacatc gagccacaac tgccggatgt ggcgacatta aaagatgttc gcctgcaaat
    15841 tccgatgcag atttacagcg ccgatggcga gctgattgct caatacggtg agaaacgtcg
    15901 tattccggtt acgttggatc aaatcccgcc ggagatggtg aaagccttta tcgcgacgga
    15961 agacagccgc ttctacgagc atcacggcgt tgacccggtg gggatcttcc gtgcggcaag
    16021 tgtggcgctg ttctccggcc acgcgtcaca aggggcaagt accattaccc agcagctggc
    16081 gagaaacttc ttcctcagtc cagaacgcac gctgatgcgt aagattaagg aagtcttcct
    16141 cgcgattcgc attgaacagt tgctgacgaa agacgagatc ctcgagcttt atctgaacaa
    16201 gatttacctt ggttaccgcg cctatggtgt cggtgctgcg gcacaagtct atttcggaaa
    16261 aacggtcgac caactgacgc tgaacgaaat ggcggtgata gccgggctgc cgaaagcgcc
    16321 ttccaccttc aacccgctct actcgatgga tcgtgccgtc gcgcggcgta acgtcgtgct
    16381 atcgcggatg ctggatgaag gatatatcac ccagcaacag ttcgatcaga cacgcactga
    16441 ggcgattaac gctaactatc acgcgccgga gattgctttt tctgcgccgt acctgagcga
    16501 aatggtgcgc caggagatgt ataaccgtta tggcgaaagt gcctatgaag acggttatcg
    16561 catttacacc accatcaccc gcaaagtgca gcaggccgcg cagcaggcgg tacgtaataa
    16621 cgtgctggac tacgacatgc gccacggcta tcgcggcccg gcaaatgtgc tgtggaaagt
    16681 gggcgagtcg gcgtgggata acaacaagat taccgatacg ctgaaggcgc tgccgaccta
    16741 tggtccgctg ctgcctgccg cagtcaccag cgccaatcct caggaagcga cggcaatgct
    16801 ggcggacggg tcgaccgtcg cattgagtat ggaaggcgtt cgctgggcgc gtccttaccg
    16861 ttcggatact cagcagggac cgacgccgcg taaagtgacc gatgttctgc aaacgggtca
    16921 gcaaatctgg gttcgtcagg ttggcgatgc atggtggctg gcacaagtgc cggaagtgaa
    16981 ctcggcactg gtgtcgatca atccgcaaaa tggtgccgtt atggcgctgg tcggtggctt
    17041 tgatttcaat cagagcaagt ttaaccgcgc cacccaggca ctgcgtcagg tgggttccaa
    17101 catcaaaccg ttcctctaca ccgcggcgat ggataaaggt ctgacgctgg caagtatgtt
    17161 gaacgatgtg ccaatttctc gctgggacgc aggtgccggt tctgactggc agccgaaaaa
    17221 ctcaccaccg cagtatgccg gtccaattcg cttacgtcag gggctgggtc agtcgaaaaa
    17281 cgtggtgatg gtacgcgcaa tgcgggcgat gggcgtcgac tacgctgcag aatatctgca
    17341 acgcttcggc ttcccggcgc aaaacattgt ccacaccgaa tcgctggcgc tgggttcagc
    17401 gtccttcacc ccaatgcagg tggcgcgcgg ctacgcggtc atggcgaacg gcggcttcct
    17461 ggtggacccg tggtttatca gcaaaattga aaacgatcag ggcggcgtga ttttcgaagc
    17521 gaaaccgaaa gtagcctgcc cggaatgcga tattccggtg atttacggtg atacgcagaa
    17581 atcgaacgtg ctggaaaata acgatgttga agatgtcgct atctcccgcg agcagcagaa
    17641 tgtttctgta ccaatgccgc agctggagca ggcaaatcag gcgttagtgg cgaagactgg
    17701 cgcgcaggag tacgcaccgc acgtcatcaa cactccgctg gcattcctga ttaagagtgc
    17761 tttgaacacc aatatctttg gtgagccagg ctggcagggt accggctggc gtgcaggtcg
    17821 tgatttgcag cgtcgcgata tcggcgggaa aaccggtacc actaacagtt cgaaagatgc
    17881 gtggttctcg ggttacggac cgggcgttgt gacctcggtc tggattggct ttgatgatca
    17941 ccgtcgtaat ctcggtcata caacggcttc cggagcgatt aaagatcaga tctcaggtta
    18001 cgaaggcggt gccaagagtg cccagcctgc atgggacgct tatatgaaag ccgttcttga
    18061 aggtgtgccg gagcagccgc tgacgccgcc gccgggtatt gtgacggtga atatcgatcg
    18121 cagcaccggg cagttagcta atggtggcaa cagccgcgaa gagtatttca tcgaaggtac
    18181 gcagccgaca caacaggcgg tgcacgaggt gggaacgacc attatcgata atggcgaggc
    18241 acaggaattg ttctgattaa aaaggcgctt cggcgccttt tcagtttgct gacaaagtgc
    18301 acttgtttat gccggatacg ggtgaacgcg ttatccggct aacaaaatcg tgaaaactca
    18361 atatattgca ggaaccccat aagcctgata agcattgtgc atcaggcaaa cttcacgcat
    18421 ttacactcgc ccctgccctt tcaaccattc gcgcacgagg aacagcgcgc tgacattacg
    18481 cgcttcgttg aagtcagggt cttccagcaa atccatcata tgcgccagcg gccagcgcac
    18541 ctgtggtagc ggctctggct catcgccttc cagcgattcc gggtagagat cttgcgctac
    18601 cacgatattc attttgctgg aaaagtaaga cggtgccatg ctgagcttct tcaaaaaagt
    18661 cagatcgttc gcaccaaatc caacctcttc ttttagctcg cggttagcgg cttcgaagac
    18721 gctttcaccc ggatcaatta atcctttcga aaaacctaat tcgtaggatt cagttcccac
    18781 tgcatattcg cggatcagga tcaggtgatc gtccacaatc ggcacaatca tcactgcttc
    18841 ccggttggtt ggacgcattc gttcataaac acgccgcacg ccattgctga actctagatc
    18901 cacgctctcg acggtaaaca gtcgggaacg ggctacagtt tcaacattca gaatggtggg
    18961 tttttgtaat gatttgctca tcgtgggatc tatgctgtga aatcagcggt tattgtgcga
    19021 tatcggacac gctttcggca atgtgaattg catgttattt acatttatgt aacttaataa
    19081 ataatcgtcc tcaaatcaaa ttaaaagtca ataggttgaa ataactccag gaatttgctg
    19141 atattccgcc ttcagagggt ttgctatgat cagcggttac tgtgatgtgc ttaatgatgc
    19201 tcaagttaaa ctccacgctt gccgatagcc aaccgcagaa tcatgtattg tgtccggtgc
    19261 gactgaccac gcctgacaga ctaagtaaga tggggaaagc atgagcacca ttgtgatttt
    19321 tttagctgct ttgctggcct gctcactact tgcgggatgg ctgataaaag tgcgatccag
    19381 acggcgtcag ctgccctgga ccaacgcctt cgcggatgcg caaacgcgta aactcacacc
    19441 tgaagaacgt agcgccgttg aaaattatct tgagagcctg acgcaggtat tacaggtgcc
    19501 tggcccaacg ggagccagcg cggcaccgat ctctctggcg ctgaatgccg aaagcaacaa
    19561 cgtcatgatg ctgacacacg ctatcacgcg ttacggcatc tctaccgacg atccgaataa
    19621 atggcgttac tacctcgatt cggtagaagt ccacctgccc cctttctggg agcagtacat
    19681 caacgatgag aataccgttg aactgattca taccgattcg ctgccgctgg ttatttcact
    19741 caacggtcat acgctgcagg agtacatgca ggaaactcgc ggctatgcct tgcaacctgt
    19801 tccgtccacg caggcgtcga ttcgcgggga agagagtgag caaatcgagc tactcaatat
    19861 tcgcaaagaa acgcatgaag aatatgcgct gagtcgtccg cgcgggctgc gtgaagcgtt
    19921 gctgatcgtc gcctccttcc tgatgttctt tttctgcctg attaccccgg atgtatttgt
    19981 tccgtggctg gcaggcggcg cgttactgct gctgggcgca ggtctgtggg ggctattcgc
    20041 ccccccggca aaatcctccc tgcgggaaat tcattgtctg cgcggtacac cccgtcgttg
    20101 gggattgttt ggcgaaaacg atcaggaaca gatcaacaat atttcactcg gtattatcga
    20161 cctggtctat cccgcacact ggcagccata cattgctcag gatctcggtc aacaaaccga
    20221 tattgatatc tatctcgacc gccacctagt gcgtcagggg cgatatcttt cgctgcatga
    20281 tgaagtaaaa aactttccgt tacagcactg gctgcgcagt acgattatcg ctgcgggctc
    20341 gctgctggtg ctgtttatgc tgttattctg gatcccgctg gatatgccgc tgaaattcac
    20401 tctctcatgg atgaaaggtg cgcagaccat tgaagccacc agcgtaaaac aactggctga
    20461 cgctggcgtg cgggtaggcg atacattgcg tattagcggt acgggaatgt gtaatattcg
    20521 cacttccgga acctggagcg cgaaaaccaa ttcacctttt ttaccgtttg actgctcgca
    20581 gatcatctgg aacgacgccc gctcattacc attaccagaa tctgaactgg tcaacaaagc
    20641 gacggcattg actgaagcag ttaatcgcca gctgcaccct aaaccggaag atgaatctcg
    20701 cgtcagcgcc tcattacgtt cagcaattca aaaatccggc atggtattgc ttgatgattt
    20761 tggcgacatt gtactgaaga cagcggatct gtgttctgcc aaagatgact gtgtgcgact
    20821 gaaaaatgcg ctggtcaatc tcggcaacag taaagactgg gacgcgctgg taaaacgcgc
    20881 caacgccggg aagctcgatg gcgtgaatgt gttattacgc ccggtgagtg cggaatcgct
    20941 ggataacctg gtggcaacct ccaccgcgcc gtttatcacg catgaaacgg cgcgagcggc
    21001 acaatcacta aacagtccgg cccccggcgg attcctgatt gtcagcgacg aaggcagcga
    21061 ttttgttgat cagccctggc cttcggcatc actttacgac tacccgccgc aagaacagtg
    21121 gaacgctttc cagaaactgg cacaaatgct gatgcatacg ccgtttaacg ccgagggtat
    21181 cgtcacaaaa atcttcactg acgccaatgg tacgcagcat ataggccttc atccgatccc
    21241 ggatcgttcc ggcctgtggc gctatctcag caccacgctg ctgctactga cgatgctggg
    21301 tagcgccatt tataatggcg tacaggcctg gcggcgttac cagcgtcatc gcactcgcat
    21361 gatgaagatt caggcctatt atgaaagctg cctgaacccg caactgatca ccccttcaga
    21421 aagccttatc gaataacacg tttgcgcggc aggttatgct accctgtcgc gcaaattgct
    21481 tcactctgga gattttcctc atgcatatca acattgcctg gcaggacgta gataccgttc
    21541 tgctggatat ggacggcacg ttgctcgacc tcgccttcga taactatttc tggcaaaagc
    21601 tggtgcctga aacatggggc gcgaaaaacg gggttacgcc acaggaagcg atggaatata
    21661 tgcgccagca atatcacgac gtacagcata cgctaaactg gtactgtctt gattactgga
    21721 gtgagcaact gggtctggat atctgtgcga tgaccaccga gatgggaccg cgtgccgtac
    21781 tgcgtgaaga taccattccg tttcttgagg cactgaaagc cagcggtaag cagcgaattt
    21841 tgctcaccaa tgcgcatccg cacaacctgg cggtaaaact tgagcatacc ggtctggacg
    21901 cacaccttga tttattactt tccacccaca catttggtta tccgaaagag gatcagcggt
    21961 tatggcatgc ggtggccgaa gctacgggtc tgaaagctga aagaacgctg tttattgatg
    22021 acagcgaagc gattctcgat gctgccgcgc aatttggtat tcgttactgc ctcggcgtga
    22081 ctaatcctga ctcagggatt gccgagaaac agtatcaacg ccatccgtca ctgaatgact
    22141 accgccgcct gatcccctcg ctaatgtgaa ggagacgcca tgaaagaaaa acctgctgtt
    22201 gaggttcgac tggataaatg gctatgggct gcccgttttt ataaaacccg cgcgctggcc
    22261 cgtgaaatga ttgaaggcgg taaggtgcat tacaacgggc agcgcagcaa gccgagcaaa
    22321 atcgtcgagc tgaatgccac gctcactctg cgccagggaa atgacgaacg cacggtgatt
    22381 gtaaaggcga ttactgaaca gcgtcgcccc gccagcgagg cagccttgct gtatgaagag
    22441 actgcggaaa gtgtagagaa acgcgaaaaa atggcgctgg cacgtaaact taatgcctta
    22501 accatgccgc acccggaccg acgcccggac aaaaaagagc gccgcgacct gttacgattt
    22561 aaacacggcg acagtgaata actgtcacct gcaagagaga tgattatgcc gcaacatgac
    22621 caattacatc gctatctgtt tgaaaacttt gccgtgcgcg gcgaactggt aaccgtttcg
    22681 gaaaccctgc aacagatcct tgagaatcac gattatccgc agcccgttaa aaacgtgctg
    22741 gcagaactgc tggttgcgac cagcctgtta accgctacgc tgaagtttga tggtgatatc
    22801 accgtacagc tgcaaggcga cggtccgatg aatctggcgg ttattaacgg taacaacaac
    22861 cagcagatgc gcggtgtggc gcgcgtgcag ggcgaaattc cagaaaatgc cgacctgaaa
    22921 acgctggtcg gcaatggtta cgtggtgatc accattaccc cgagcgaagg tgaacgctat
    22981 cagggcgtgg tgggtctgga aggtgatacc ctggcggcct gcctggaaga ttactttatg
    23041 cgttctgaac agctgccgac gcgcctgttt attcgcaccg gcgacgtaga cggcaaaccg
    23101 gctgcaggcg gtatgttgtt gcaggtaatg cctgcgcaaa atgcccagca ggacgacttt
    23161 gaccacctgg cgacgctaac cgaaaccatc aaaaccgaag aactgctgac cttaccggca
    23221 aacgaagtgt tgtggcgttt gtatcacgaa gaagaggtga cggtttacga tccgcaggat
    23281 gtggagttca aatgcacctg ctcgcgtgaa cgttgcgccg atgcgctgaa aacgctgcct
    23341 gatgaagaag tcgatagcat cctggcggaa gatggcgaaa ttgacatgca ttgtgattac
    23401 tgcggtaacc actatctgtt caatgcgatg gatattgctg aaatccgcaa caacgcgtct
    23461 ccggcagatc cgcaagttca ttaatgagta tgtccggcag agagagctct gccggatttt
    23521 acgactaata cacctgatgc gctttgtcac tcattcagat tcacatccag cctttcacca
    23581 tccactacaa gatcgcgcca cgctttaggc ttcgttccca gttttccatc cttcgcagct
    23641 gtgagtaatt tttctttcgt tatatcacgc ggaagtaaac tcatcgtaag ctcttgccat
    23701 tctttcttgt caggatcaaa gccatagaca atgtatcttt catcatcaaa ggcaaagagg
    23761 atccgctctg ccctgccatc actattcaga tcttgctcga ccagtacgca ggcgtctttt
    23821 tcaatacagg tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc
    23881 ggtttaccag aaccaggggc aattaacaca ttttcggcta atactttttc cgatatctgt
    23941 tgctgaagat gttgctctcc atctaacgcc atcagtagat cccgcgcgcg tttcgggtct
    24001 ttcatatact cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctg
    24061 tgttcgagca tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc
    24121 atatgactgt tcacactaat gcgcatactg tccagcaccg gcgaattaag aagcaccagt
    24181 attaccaaca ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt
    24241 tgccctttac gccagacgat actgacaaaa taccccagcg accataccag taacaccagc
    24301 accgccagcg cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat
    24361 aacgcccagg cggcgatgaa cacatacagc ggagcaacca gcagagcggt tttaatcagg
    24421 caacgcaacg gccccgtcca gggaagtgac gctttttgcg gatcgcggac aatagccatt
    24481 aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa
    24541 atcgcactca ggcctgtaaa cggcagggtg atgataaaca tcagggttag caatgatact
    24601 aacggcagca accccgtggc gattaacgtg aacaactttt gaatagagtc gattaaacgt
    24661 gactgtgttc gcgcgaggat caccgccagc gcggtaacca gacctaacgt gagataaata
    24721 aaccagtcgg ttgcaaaaaa gagcgttttg aaaaacgtga tgccaacaag tttaaacaac
    24781 tcactccaca gtaaaagcac cagccacgtc agaccattgg cgagaaaaat caccagtaat
    24841 ataagtacgt tatgccatac tgactggtaa aaatagcgat agcggctact gtcattgcgg
    24901 atgcgaaggc tttgttgtat ccacggtagc aacaacattg ccatcagcag cagatagcaa
    24961 ccaaaatccc aaagcgcctt ttcagctctc cagggggtca tgccatcagt ttgccacttc
    25021 agccagccgc tcatccccag cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc
    25081 ttctgtttaa aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc
    25141 acgccataaa caatccaact gtgatttttt ccggaaagcc aggcgatcag caggtagcaa
    25201 accagaccct gaagtaatcc ggtagcaatc atcccccagc gtgttgcggg tgaaagttca
    25261 acgttatcca tgttggttat ccagaatcaa aaggtgggtt aattatcgca tccgggcagt
    25321 agtattttgc ttttttcaga aaataatcaa aaaaagttag cgtggtgaat cgatacttta
    25381 ccggttgaat ttgcatcaat ttcattcagg aatgcgattc cactcacaat attcccgcca
    25441 tataaaccaa gatttaacct tttgagaaca ttttccacac ctaaaatgct atttctgcga
    25501 taatagcaac cgtttcgtga caggaatcac ggagtttttt gtcaaatatg aatttctcca
    25561 gatacgtaaa tctatgagcc ttgtcgcggt taacaccccc aaaaagactt tactattcag
    25621 gcaatacata ttggctaagg agcagtgaaa tgcgcgttaa caatggtttg accccgcaag
    25681 aactcgaggc ttatggtatc agtgacgtac atgatatcgt ttacaaccca agctacgacc
    25741 tgctgtatca ggaagagctc gatccgagcc tgacaggtta tgagcgcggg gtgttaacta
    25801 atctgggtgc cgttgccgtc gataccggga tcttcaccgg tcgttcacca aaagataagt
    25861 atatcgtccg tgacgatacc actcgcgata ctttctggtg ggcagacaaa ggcaaaggta
    25921 agaacgataa caaacctctc tctccggaaa cctggcaaca tctgaaaggc ctggtgacca
    25981 aacagctttc cggcaaacgt ctgttcgttg tcgacgcctt ctgtggtgcg aacccggata
    26041 ctcgtctttc cgtccgtttc atcaccgaag tggcctggca ggcgcatttt gtcaaaaaca
    26101 tgtttattcg cccgagcgat gaagagttgg caggtttcaa accagacttt atcgttatga
    26161 acggcgcgaa gtgcactaac ccgcagtgga aagaacaggg tctgaattcc gaaaacttcg
    26221 tggcttttaa cctgaccgag cgcatgcagc tgattggcgg cacctggtat ggcggcgaaa
    26281 tgaagaaagg gatgttctcg atgatgaact acctgctgcc gctgaaaggt atcgcttcta
    26341 tgcactgctc cgccaacgtt agcgagaaag gcgatgttgc ggtgttcttc ggcctttccg
    26401 gcaccggtaa aaccaccctt tccaccgacc cgaaacgtcg cctgattggc gatgacgaac
    26461 acggctggga cgatgacggc gtgtttaact tcgaaggcgg ctgctacgca aaaactatca
    26521 agctgtcgaa agaagcggaa cctgaaatct acaacgctat ccgtcgcgat gcgctgctgg
    26581 aaaacgtcac cgtgcgtgaa gatggcacta tcgactttga tgatggttca aaaaccgaga
    26641 acacccgcgt ttcttatccg atctatcaca tcgataacat cgttaagccg gtttcaaaag
    26701 cgggtcacgc aacgaaagtt atcttcctga ccgcagacgc ctttggtgtg ctgccaccgg
    26761 tttctcgtct gactgccgat caaacccagt accacttcct ctctggcttc accgccaaac
    26821 tggccggtac tgagcgtggc atcaccgaac cgacgccaac cttctccgct tgcttcggcg
    26881 cggcattcct gtcgctgcac ccgactcagt acgcagaagt gctggtgaaa cgtatgcagg
    26941 cggcgggcgc gcaggcttat ctggttaaca ctggctggaa cggcactggc aaacgtatct
    27001 cgattaaaga tacccgcgcc attatcgacg ccatcctcaa cggttcgctg gataatgcag
    27061 aaaccttcac tctgccgatg tttaacctgg cgatcccaac cgaactgccg ggcgtagaca
    27121 cgaagattct cgatccgcgt aacacctacg cttccccgga acagtggcag gaaaaagccg
    27181 aaaccctggc gaaactgttt atcgacaact ttgataaata caccgacacc cctgcgggtg
    27241 ccgcgctggt agcggctggt ccgaaactgt aatgatttga agctggagaa tatctatcca
    27301 gtatcttata gaaagcaaaa cgggaggcac cttcgcctcc cgtttattta cccttctttt
    27361 gtcgtgctct gcgcccgcgt taccggcaca ggcagccagg cgcgaatgga aagcccgccc
    27421 cgctcgctgg tgccaagctc cagcatcccg ttatggttat ccacgatacg ctgcacaatc
    27481 gccagcccta atcccgtgcc gctaatggtg cgtgcactgt cgccgcgaac aaacggctgg
    27541 aaaaggtgct tacgttgttc cggcgcaatt cccggaccgt catcttccac ctggaaccag
    27601 gcgcgattcg gctccgtccc gctgctgact ttgatccagc cattgccgta acgggcggcg
    27661 ttgaccacca tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt cacttcaatg
    27721 ctgccggggt aaagcgcggt ttcaatttcc cgctcatagc cactttcggc agcaataacc
    27781 tcaccgagta ctgcgttgag atccgccatt tccatcggca tctcctgtcc ggtgcgcagg
    27841 tagtcgataa actgctcaat gatggcattg cactcctcga tatctttatt gatcgattct
    27901 gccaggtagc catcctgctc gctcatcatc tcggtcgcca ggcgaatacg cgtcagcggt
    27961 gtacgcaagt cgtgacttac ccccgccatc agcagcgtgc ggtcatccgc cagttgctta
    28021 acaccagccg ccatatggtt aaaggcgcgg gtaacggaac gcacctcgga cgcgccatac
    28081 tcacgcagcg gcggcggaat aatcccttta ccaacctgca aggctgcgtg ttcgagatcg
    28141 accaacggtc gattctggat acgaataaac agccacgccc cgcctatcgc caatagcata
    28201 atcgccaacg tatagcggaa cagcggagag aaatcgccct gatgaatttc ggtcagcggc
    28261 acgcgtaccc agatattggg cgacagccag gttttcagcc agacgacagg cgaacttttg
    28321 ttgacctcaa cgcgcacttc cgtcgggccg cccagttgct gtgccatctg atggcttaag
    28381 aattcatagt gttgcgccca acgcagacct gcctcttcgg cagcctcgtt ggagtagaga
    28441 gagatcccca gctcacggta gatctcccga cggaaagcgg gaggcacaac caactgcgtg
    28501 ccgtcctcca gttgcagttt gtcggtcatc aacatacgca cttcgtacgc gaggacttta
    28561 ttaaactgct ggaggctcgg caaaatcgcg aagttcagca ccaccagata agtcgtcacc
    28621 aggctggcga acagcaaggt gacgatgagc aataacgtac gggcaaatga acttcgtggc
    28681 gagaagcgca atcgcctcat gctttagagc cgtccggtac aaagacgtag cccaggcccc
    28741 aaacggtctg aatgtaacgc ggatgcgctg gatcttcttc caccatgcgg cgcagacgag
    28801 aaatctgcac gtcgatggag cgttccattg cggaatattc acgaccacgg gcaaggttca
    28861 tcagcttatc gcgggagagc ggctcacgcg gatggctgac cagtgccttc agtaccgcaa
    28921 actcaccgct ggtgagcggc atcggctcgt cttcgcggaa catttcgcgc gtaccgaggt
    28981 taagtttgaa cttaccgaaa gcaattaccg cctcttcctg tgacggtgcg cctggtagct
    29041 cgttcgcctg acgacgcagc accgcacgga tacgggccag cagttcacgc gggttaaacg
    29101 gttttggaat gtaatcgtca gcgccaatct ccaggccaac gatacggtcc acttcttccc
    29161 ctttcgccgt caccataatg atcggcatcg ggttgctctg actacgaaga cgtcggcaaa
    29221 tcgacaagcc atcttcacca ggtaacatta aatccagtac cataagatgg aaagattcac
    29281 gagtcagcag gcgatccatc tgttctgcat tagcgacgct tcgaacctgg aagccttgtt
    29341 cggtgagata acgttccagc agcgcacgca ggcgcatgtc gtcatcgacc accagaatct
    29401 tgtagttctc ttgcattgtt tgtactccca aaggttcgca acaatttgta agcgtgtatt
    29461 cttaaaaaag ctcacgttcg tcaccagcta aatctggtat gaatttcagc ctaaattgtt
    29521 acaaagcata ttaaacagca gcttaagtat acaatttatt cggcgaaaca ttattgattc
    29581 tgttgatatg atcacgttat acccaatgtg cgcattatca aacagacaaa gggaatcaac
    29641 gagatgaaaa cgcccctggt tacccgggaa gggtatgaaa aactcaaaca agagcttaat
    29701 tatctctggc gtgaagaacg cccggaggtc acaaaaaagg tgacctgggc cgcaagtctg
    29761 ggcgaccgca gcgaaaatgc tgactatcag tataataaaa agcgcctgcg tgaaatcgac
    29821 cgtcgcgtgc gctatctcac taaatgcatg gaaaatctca aaatcgtcga ttactccccg
    29881 cagcaggaag gcaaagtctt ttttggcgcg tgggtggaga ttgaaaacga cgatggcgtg
    29941 actcaccgtt tccgtattgt cggctacgat gaaatttttg gccgtaaaga ttacatctct
    30001 atcgattccc cgatggcccg cgcattgctg aaaaaagaag tcggcgatct ggctgtggtg
    30061 aatactcctg ccggggaagc gagctggtat gttaatgcta tcgagtacgt gaaaccgtaa
    30121 ggaagagtct taacctcctg ccgatggctg gcatttttgc cagccagtcc gtataactat
    30181 cccctgattt ttgatccgaa aagatgaact caaaccatga tgaatgattc gttctgccgc
    30241 attattgcgg gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt tcgcctgctt
    30301 gacgaaggga ataccgtgcc ttttattgca cgttatcgta aggaaatcac cggcggtctg
    30361 gatgacacgc agctgcgtaa tctggaaacg cgtctgggat acctgcgcga gctggaagag
    30421 agacgtcagg cgatcctcaa gtccatttcc gagcaaggca aactcaccga tgatctggcg
    30481 aatgccatca acgccaccct aagcaaaacc gaactcgaag acctctacct gccctacaaa
    30541 cctaaacgcc gcacccgcgg gcaaatcgcc attgaagcag ggcttgagcc gttggctgac
    30601 ctgctgtgga gcgatccgtc acacacgcca gaagtcgccg ctgcgcaata tattgatgcc
    30661 gataaaggcg tggcagatac caaagccgcg ctggacggcg cgcgctatat cctgatggaa
    30721 cggtttgccg aagatgccgc gctgctggcg aaagtgcgtg attatctgtg gaagaacgcg
    30781 catttggttt ctacggtggt gagcggtaaa gaagaggaag gggcgaaatt ccgcgactat
    30841 ttcgatcatc acgaaccgtt gtccacggtg ccttctcacc gcgcgctggc gatgttccgt
    30901 gggcgtaacg aaggcgtact tcagctttcg ctgaatgccg atccacagtt cgatgagccg
    30961 cccaaagaga gctattgcga gcaaatcatc atggatcacc ttggcctgcg cctgaacaat
    31021 gccccggcgg atagctggcg caaaggcgtg gtgagctgga cgtggcgcat caaggtgctg
    31081 atgcatctgg aaaccgaact gatgggcacc gtgcgcgaac gtgcggaaaa tgaagcaatc
    31141 aacgtctttg cccgtaacct gcacgatctg ctgatggctg cccctgccgg actgcgtgca
    31201 acgatgggcc tcgatccggg tctgcgtacc ggggtaaaag tggcggtggt cgatgccact
    31261 ggcaaactgg tagcgaccga taccatttac ccgcacaccg gacaagccgc aaaagcagcg
    31321 atgactgttg ctgcgctgtg tgaaaagcat aacgttgaac tggtggcgat cggcaacggt
    31381 acagcttccc gcgaaaccga acgtttctat ctcgacgtgc agaagcagtt cccgaaagtg
    31441 accgcacaga aagtgatcgt cagcgaagct ggcgcgtcgg tttactcggc ttccgagctg
    31501 gcagcgcagg agttcccgga tctcgacgtt tcgctgcgtg gcgcggtttc tatcgcccgc
    31561 cgtttgcagg atccgctggc ggagctggtg aaaatcgatc cgaaatctat cggcgtaggt
    31621 cagtatcagc atgacgtcag ccagacgcaa ctggcccgca aactggatgc agtagtagaa
    31681 gactgcgtaa acgccgttgg cgtcgatctc aacaccgctt ctgttccgct attaactcgc
    31741 gtggcgggcc tgacgcgaat gatggcgcaa aacatcgtgg cctggcgcga tgagaacggc
    31801 cagttccaga accgtcagca actgctgaaa gtcagccgtc tggggccgaa agccttcgag
    31861 cagtgcgcgg gcttcctgcg cattaaccac ggtgacaacc cgctggacgc ctctaccgtt
    31921 cacccggaag cctatccggt ggtggaacgc attctggcag caacacagca ggcactgaaa
    31981 gatctgatgg gtaacagcag cgaactgcgt aacctgaaag cgtctgactt tactgatgaa
    32041 aaattcggtg tgccgacagt aactgacatc atcaaagagc tggaaaaacc gggtcgcgat
    32101 ccgcgtccgg aatttaaaac cgctcagttt gccgatggcg tcgagacaat gaacgacctg
    32161 caaccgggta tgatcctcga aggcgctgtc accaacgtca ccaactttgg cgcgtttgtc
    32221 gatatcggcg tgcatcagga cggcctggtt cacatctctt cattgtcgaa caagtttgtg
    32281 gaagatccgc ataccgtggt gaaagcgggc gacattgtga aggtgaaagt gctggaagtg
    32341 gatcttcagc gtaaacgtat cgccctgact atgcgtctgg acgagcagcc tggcgaaacc
    32401 aactctcgtc gcggcggcgg taacgatcgc ccgcaaaaca accgcccggc agccaaacca
    32461 cgcggtcgtg aagcgcagcc tgccggtaac agcgcgatga tggacgcgct ggcggcagca
    32521 atgggcaaaa aacgttaaac gcccggactg gcccctaaac ctgcgcgata aggcgttcac
    32581 gccgcatctg gcaacgaacg ccttgcctga cataaaagtg ccggagaata tctccggcat
    32641 atttattcca cagccaaact cacaatatat ttcgttaata tttatcactt cattaacaac
    32701 tgaaacctta attaaacatt agccagtcct ggtaattcgc tattcgaatt atattttcgc
    32761 tgcgatataa ccttgagcca catcaacatt gagtcagatt attattcaaa ccaacattcg
    32821 cacacatttt aagtattgct gatagaaacc attctcatta tcattgtact attgattatt
    32881 taatctctcc ttcgttggca aatcatctgg tctcatgtcg ctgtcaaacg ccccatgagg
    32941 tagttatcca gttaatgaga aacaagtagg cacctatgca atacactcca gatactgcgt
    33001 ggaaaatcac tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa ctgctttctc
    33061 ttggcatgtt acctggctcc tcttttaatg tggtgcgcgt cgctccactc ggcgacccca
    33121 ttcatatcga aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg gccttattag
    33181 aagtggaagc ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt aaccataggc
    33241 ttaattggta atccaaattc tggcaagaca accttattta accagctcac tggcgcacgt
    33301 cagcgtgtag gtaactgggc tggcgttacc gtcgaacgta aagaagggca attctccacc
    33361 accgatcatc aggtcacgct ggtggacctg cccggcacct attctctgac caccatctca
    33421 tcgcagacct cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg cgacgccgac
    33481 ctgctgatta acgtggtgga tgcgtctaac cttgagcgta acctgtacct gacgctacaa
    33541 ctgctggaac tcggcattcc ctgcatcgtg gcactgaaca tgctcgacat tgccgagaag
    33601 caaaatattc gtattgaaat tgatgctctg tcggcgcgtc ttggctgccc agtgatcccg
    33661 ctggtttcaa cccgtggtcg cggtattgaa gcgctcaagc tggcgattga tcgctataaa
    33721 gctaacgaga atgtggaact ggtgcattac gcacagccgc tactcaacga agcagattca
    33781 ctggcaaaag tgatgccttc cgacatcccg ctgaaacaac gtcgctggct gggcctgcaa
    33841 atgctggaag gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca gcatcttgat
    33901 gccgccctcg cccgtctgcg taatgagatg gacgatccgg cgctgcacat tgccgatgcg
    33961 cgttaccagt gcattgctgc catctgtgat gtggtaagca acaccctgac ggcagaaccc
    34021 agccgtttca ccactgcggt agataaaatc gtgctcaacc gtttcctcgg tctgccgatt
    34081 ttcctctttg tgatgtacct gatgttcctg ctggctatca acatcggcgg ggcgttacag
    34141 ccgctgtttg acgtcggctc cgtggctcta tttgtgcatg gtattcaatg gattggctac
    34201 acgctccact tcccggactg gctgactatc ttcctcgccc agggcctggg tggcggtatt
    34261 aacaccgtgc tgccactggt gccgcagatc ggcatgatgt acctgttcct ctccttcctt
    34321 gaggactccg gctatatggc gcgtgcggcg tttgtgatgg accgcctgat gcaggcgctg
    34381 ggcttgccgg ggaaatcctt tgtgccgctg atcgtcggtt tcggttgtaa cgtaccgtcg
    34441 gtaatgggtg cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat catgatggca
    34501 ccgtttatgt cctgcggcgc gcgtctggct atcttcgcag tattcgcggc tgccttcttc
    34561 gggcagaacg gtgcgctggc ggtcttctcg ctgtatatgc tcggtatcgt gatggcggtg
    34621 ctgactggcc tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc gtttgtcatg
    34681 gagctgccgg tctaccatgt accacacgtt aaaagcctga ttatccagac ctggcagcgt
    34741 ctgaaaggct tcgttctgcg tgctggtaaa gtgattatca tcgtcagcat tttcctgagc
    34801 gctttcaaca gcttctcgtt gagcgggaaa atcgtcgata acatcaacga ctcggcgctg
    34861 gcttccgtca gccgggtgat caccccggtc ttcaagccga ttggcgtgca tgaagataac
    34921 tggcaggcaa cagttggcct gtttacgggt gcaatggcga aagaagtggt ggtgggtacg
    34981 ctcaacaccc tctacaccgc agaaaatatt caggacgaag agttcaatcc ggcggaattt
    35041 aacctcggtg aagagttgtt cagcgcggta gatgaaacct ggcagagcct gaaagacacc
    35101 ttcagcctta gcgtactgat gaaccccatt gaagctagca aaggcgacgg cgaaatgggt
    35161 accggggcga tgggcgtgat ggatcagaaa ttcggtagcg cagctgccgc atacagctac
    35221 ctgattttcg tcctgctgta tgtaccatgt atctcggtga tgggagccat cgcccgtgaa
    35281 tcaagccgtg gctggatggg cttctccatc ctgtgggggc tgaatatcgc ttactcactg
    35341 gcaacattgt tctatcaagt cgccagctac agtcagcacc caacttacag cctggtgtgc
    35401 attctggcgg ttatcctgtt taacatcgtg gttatcggtc tgctgcgccg cgcgcgtagc
    35461 cgggtggata tcgaactgct ggcaacccgc aagtcggtaa gtagttgctg cgcagccagc
    35521 accaccggtg attgccatta atggcttcac ttattcaggt gcgtgatttg ctggcgttac
    35581 ggggccgtat ggaagcggcc cagataagcc agacattgaa cactccacag ccaatgatta
    35641 acgccatgct gcaacaactg gaaagtatgg gcaaagccgt gcggattcag gaagaacctg
    35701 acggctgcct ctccggcagt tgtaaaagct gcccggaagg aaaagcctgt ctgcgcgagt
    35761 ggtgggcgct gcgttaacca tactccatcg cctgttttga aaagcccggt atgcgtttgc
    35821 atccgggctt ttttgcgtgc ggctttccat aaaaatgcaa ttcttgcagc gcgacgtaag
    35881 ttcctctgaa agcgtctcgc agggatgaaa actggctaat gcacaggtgt ggagtggcgc
    35941 gtagagtcgc ggcattcaaa caacaggtga aggaacgcca tgagcaaaaa gcagagttcc
    36001 accccacacg atgcgctgtt caaactcttt ttacgccaac cggagacggc acgcgatttt
    36061 cttgcgtttc atttaccagc acccattcac gcgctctgtg atatgaaaac cctcaagctg
    36121 gagtcgagca gctttattga tgacgatctg cgtgaaagct attccgatgt gctgtggtcg
    36181 gtgaaaacag aacaaggacc aggatacatc tattgtctga ttgaacatca aagcacctca
    36241 aacaaactga tcgcatttcg catgatgcgt tacgctattg ccgcaatgca aaatcacctt
    36301 gatgccggat acaaaacgtt gccgatggtg gtgccattgt tgttttacca cggtattgaa
    36361 agcccctatc cctattcgct gtgttggctg gattgtttcg ccgatcccaa actggcaagg
    36421 cagctttatg cctccgcatt tccgctgatt gatatcaccg tcatgcctga tgatgaaatc
    36481 atgcagcacc gacgcatgac gctgctggag ttaattcaaa aacatattcg tcaacgcgac
    36541 ctgatggggc tggtagagca aatggcctgc ttattaagta gtggatacgc taatgacaga
    36601 caaatcaaag ggctgtttaa ttacatactg caaaccggcg atgcggtacg ttttaacgat
    36661 tttatcgacg gcgttgccga acgatcaccg aaacacaagg agagtttaat gactattgcg
    36721 gaaagattgc ggcaggaggg ggaacaatcc aaagccctgc atatagccaa aataatgctt
    36781 gaatccggag tccctcttgc agacatcatg cgctttaccg ggctgtcaga agaagagttg
    36841 gctgcggcga gtcagtaaag ttctgtctcg ccattcaaaa gtgcctacac cctctgcttc
    36901 aacgccacca gcacgtgacg aaactcgacc ggatgcgaaa taaacggcgc atgagccgct
    36961 ttggcgaaga tgtatgactc gctgtgtggc cagagtttat ccagcatcgg caccactttg
    37021 cgcggcacca gaccatcgag atagccatac agtcgcaaaa acggcatggg tacgttttgc
    37081 agcggcaagc ggagatcaac cgttttgagg atttccagcc caccattgag cacgtcaacc
    37141 tccggcatcg gtaacgccag aacggttttc ttcaacgccc gcgcatcctg acgcgccgtt
    37201 tcagtaccca tggtttgtaa cgcaaggaac cgttccaccg tacgctgaaa atcatcactg
    37261 agttgctgct gaaatcccgc cagcacgtcc ggttttatcc ccggccactc gtcacgagca
    37321 ctaaaacaag gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg atgggttaac
    37381 gcaatctggc ttgccaccag cccgcccaga ctccagccta accaaatggc tttatcaggt
    37441 gcctgtcgca gcacggcttc ggccatctcc gcaagtgaca gcgcaccaaa tccccggcta
    37501 cgcccgaagc cgggcaggtc aacaagatgc agcgtaaaat gcgagctaag ttcctcgtca
    37561 atgcaacgcc acacttcagc attcagtccc catccgtgca gcagcacaag atgaacattc
    37621 ccctgacctt tggtctgcca ccagatgtta ttcatccgct attgttctct tttgacttac
    37681 aaggatgaac atatgctaac agtaccggga ttatgctggc tatgccgaat gccactggca
    37741 ttaggtcatt gggggatttg ttcggtctgc tcacgcgccg cccgtacaga taaaacgtta
    37801 tgcccacaat gtggattacc cgccacacac tcccatcttc cctgcggtcg ctgcctgcaa
    37861 aaaccgccgc cctggcaaag actggtcacg gttgccgact atgcgccgcc gttaagtctg
    37921 ctcatccacc agcttaagtt ttcccggcgc agcgaaatcg ccagcgccct gtcacgtctg
    37981 ttactattgg aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc ggatcgtatc
    38041 atcagcgttc cgttatggca gcggcgtcac tggcgtcggg gatttaatca gagcgatttg
    38101 ctgtgtcagc cgttatcacg ctggttgcac tgccaatggg atagcgaagc catcacacgt
    38161 acacgggcca ctgcgaccca gcattttctt agcgcccggc tacgcaggcg caacctgaaa
    38221 aatgcctttc gccttgaatt gcctgtgcaa ggtcgccata tggtgattgt ggatgatgtc
    38281 gttaccaccg gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg
    38341 actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatgg gcgtattata
    38401 accaactaaa atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca
    38461 agcgcacttt gccaaactgc tggcaaatca ggaagaaggg acacaaatcc gcgtatttgt
    38521 gattaaccct ggcacgccta acgctgaatg tggtgtttct tattgtccgc cggatgccgt
    38581 ggaagccacc gacaccgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag
    38641 cgcaccatac ctggaagatg cagagatcga ttttgttacc gaccagttgg gttctcagct
    38701 aacactgaaa gcgcctaacg ccaaaatgcg taaagtggca gacgatgcac cactgatgga
    38761 gcgcgtggag tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggccg
    38821 cgtttcactg atggaaatca ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg
    38881 taacggttgt tccatggtcg atgtgacgct gaaagaaggg atcgagaagc agctactaaa
    38941 cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc
    39001 ctactactaa gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc
    39061 gggcctacgt tgaacatgtg ccagacgttg gcgcagcatt gctttggcaa atccctccct
    39121 tcgtttcccc gcgataatat gaccaacctc tcataattta aatttacccc gctctggtga
    39181 ttctcaaacg ccagatgtta cccgtatcat tcacgtgggt accaaacata ctcctgacat
    39241 ctgactacaa taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc
    39301 gattgggata attagagctt gtcgtcagaa aattgacgtt acccataaca aatgaaaggc
    39361 caggtaaatc atgccattag tcattgttgc tatcggtgta atcttgttat tactcctgat
    39421 gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg cggttggatt
    39481 aatgcaaggg atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcgggac
    39541 gctcggtagc cttgccctga tcatgggttt tggcgcaatg ctgggcaaaa tgctggcaga
    39601 ctgcggtggc gcacaacgta tcgccaccac gctgattgcc aaatttggta aaaaacacat
    39661 ccagtgggcg gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg
    39721 ctttgtgctg atgctgccgc tggtgtttac catcgcggct tctgcgaata ttccactgct
    39781 gtatgttggt gtaccaatgg cagctgcact gtctgtgacc cacggcttcc tgccaccgca
    39841 tccgggtccg actgcgattg ccaccatttt caatgccgat atgggtaaaa ccctgctgta
    39901 cggtactatt ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt
    39961 gaaaggtatc gataagccaa ttccggaagg tctctacagc gcgaaaacct tcagcgaaga
    40021 agagatgccg agctttggcg tcagcgtctg gacctctctg gtgccggtag tactgatggc
    40081 gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga
    40141 gttcctcggt gacccggtaa tggctacgct gattgccgtg ctgattgcga tgttcacctt
    40201 tggtctgaac cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa
    40261 aatcattgcg atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt
    40321 agacagcggc gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct
    40381 gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc
    40441 ggcaatcact gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga
    40501 gctgatggtt attgcggtgg gttccggtag tgtgattttc tctcatgtga acgatccggg
    40561 cttctggctg ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc
    40621 gatgctggaa accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt
    40681 gatttgataa cactgactgc cggatgcatc gcatccggca tcaaccgcac tctacttctt
    40741 cttcgctgca gctctgcgcc gtctgtccaa atccttcagc aacttgttca cgccatcatc
    40801 ggcaaacatc gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat actggtaact
    40861 ggtgccagga atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa
    40921 cagagcactg ttactgtcgg caatgtagcg ctgcaaacca cgattaagcg tcggcgtcat
    40981 cgacatcaac gatgccttat gcccggcacg ttttggcaga caaccatatt tatgcagtgc
    41041 atccagcagc ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc
    41101 atccggatac agccccaggg ttttgcccag cgttagatcc ccgctctccc agtagccgcg
    41161 tagcgtaggc aggtcatgtg tcgccgcaac cgccatcgac tgctccggat acgctttcgg
    41221 tgcacggaac gtcttctcgt ggtcgttttc gaaatagagc actttgtagg agtacacacc
    41281 gctgctgcgc agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac
    41341 catacagcga tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata
    41401 gtgaacatac gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat
    41461 cgacatcaca tggtcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc
    41521 gataaacggt tcataagcac gcgcggtgat gatatgcggg tccattggcg gcaatcccca
    41581 gttctgcccc aacgggccga ggatatccgg cggcgcgcca accgacgctt tcaggcaata
    41641 cagttcacgg tcgcaccagg tttccgcccc accttccgct acgccaaccg ccagatcacg
    41701 atacaagcca atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct
    41761 gtaagcctgc cactgcaacc agagataaaa atcaacgtca tcgcgatgtt cttcgcagaa
    41821 ctgacgcact tctggtgaat ccacgttctg atacatctct ggccatgcag gccagcccca
    41881 gcgcatttcg tcctctttca cttgctgggc atgtagcgca tcaaaggctg cctgccagaa
    41941 caggctgtcg ccttgatctg caacaaactg gcgaaacgcg gccatctgct catcatcacg
    42001 ttgcgcgaaa cctttccacg ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga
    42061 gtaatcgacc cagtcggcat cccgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactg
    42121 ccaccaggcc tgagcctctt cgctaagatg gaaatcttcg acggcgttaa cgtcgatata
    42181 aatcacattc agccagcggc gagaagacgg gctatacggg ctggcgctct ccggatttgc
    42241 cggatagagc gcatgaatcg ggttcagacc aatgaacgat ccgccacgtt ttgccacatc
    42301 cactagcatc gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag
    42361 cgtataaagc tggacgcagg caccccacag cttttgttta ttcagcaacg cctgcggttc
    42421 gtaacagcgt ttcggggcga caatcaccct gcaatgcgcg cgctggtctt cctgggtaag
    42481 cgtcagcgtg tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt
    42541 tatatggcct ttgtactgcg ttccttcttc ggtggtcagc agccagctat attcgccgct
    42601 gccctccacc accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg
    42661 cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt
    42721 ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg taatgagggc taatccccgc
    42781 cgccagcgcg gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg
    42841 ccagatacga gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc
    42901 ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg
    42961 cttttgtgcc tctacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccctg
    43021 tttgccgata ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc
    43081 gctttccagc tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg
    43141 gtcgtagcct ttggccagaa ttgccttcac ttgttccacg gtatgaccaa aaataaagat
    43201 attttcttca ccgactttct cggcgatttc aacgttcgcc ccatccagcg taccgacagt
    43261 aagcgcacca ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgcagt
    43321 cgaaatttgt tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata
    43381 atccggcagg aacaccacct tcaacttatc gccaaccagc ggatcgttgt tgatcacgtc
    43441 agccactttg ttgatcgcaa agataatatt tttcgccagg tagtagcccg gtgccgcttt
    43501 cgcgccgaag aggaagacgc gcggtacgcg atcagcctgt gggttttcac gaatttcttt
    43561 gtacaacgcc agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt
    43621 gatctgaata tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa
    43681 ctccgccaga cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc
    43741 atcatcagcg aatttttcca gattgatcag ctgatcgaga tcgttagccc actctttttt
    43801 cagtgattta tccaacagag ccgccagtgc cgggttgcac tgtttgatcc agcgacgtgg
    43861 ggtaataccg ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa
    43921 cagatctttc accaccagat ccgagtgcag cgccgcaaca ccgttcaccg cgaaaccgcc
    43981 aaccacacac aggttcgcca tatgcacttg tttgtcgtgc accaccgcca gtttagccca
    44041 cactttttca tcgcccggcc aggttttctc caccagcgtt ttaaagcgag tattaatttc
    44101 gttaataatc tgcatgtggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc
    44161 cagcgcttct ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg taatggccca
    44221 ggcgtcatcc cagctcatct ggtgctcatc gatcagcacg cgcaacagtt ctggaatcgc
    44281 gatagttggg tgggtatcgt tcagctgaat aacttcgtaa tccgccagtt cgtgcagttt
    44341 acgccccgcc agatgatggc gacgcaaaat atccgctacc gaacaggcac actggaagta
    44401 ctgctgcatc aagcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt
    44461 ggtcagtttt tccgcattga tgccctgctg ttcggcacgc aggaaatcac cgtcgttaaa
    44521 tttagtcaga tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg gctgcgccac
    44581 gccattacga tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg
    44641 ctcccagcgt ccgtctttcg tcactttacc gccaatccct acctgcacat ccagtgcttc
    44701 gttgtggcgg aaccacgggt agttactgcg atgccagtca tccggcgctt caacctgttt
    44761 gccatccaca aaagactggc ggaacaaacc atattgatag ttcagaccgt aacccgtcgc
    44821 agactgaccg acagttgcca ttgaatcgag gaagcacgcc gccagacgtc ccagaccacc
    44881 gttacccagc gcagggtcga tctcttcttc cagcaggtca gtcagattga tgtcataagc
    44941 cttcaacgaa tcctgtacat cctgatacca gccgagattc aacaggttgt tgcccgtcag
    45001 acgaccaatc aaaaactcca ttgagatgta gttaacatgt cgctgattcg ccaccggctt
    45061 ggcgaatggc tgagcacgca gcatttcggc cagtgcttca ctcactgcca gccaccactg
    45121 gcgaggagtc atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc
    45181 ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga
    45241 ttttcaacac aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg
    45301 ggggattagg cagggaggag ttgcggggat gagcaaggaa atgtgatctc gaccacttaa
    45361 agctagtgca aaccacagga ttagcagcaa atcaatgcca tacagcgcag aaaatctgta
    45421 tctaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc gaaaggtaac tctataaatt
    45481 aagtaaagga gtgaaacagt ttcacaagta aaatatccag tgtgctccat ctcattctta
    45541 atagatttat taagatcatc tttttagata gcactttcat caggaatgaa gaagaaaccc
    45601 ttgcttaaat gaatctgatg aacataaggg aaaccagtat tcacgctgga tcagcgtcgt
    45661 tttaggtgag ttgttaataa agatttggaa ttgtgacaca gtgcaaattc agacacataa
    45721 aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac cttataacca ttaattacga
    45781 agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa gtgattaact atgctgattc
    45841 cgtcaaaatt aagtcgtccg gttcgactcg accataccgt ggttcgtgag cgcctgctgg
    45901 ctaaactttc cggcgcgaac aacttccggc tggcgctgat cacaagtcct gcgggctacg
    45961 gtaagaccac gctcatttcc cagtgggcgg caggcaaaaa cgatatcggc tggtactcgc
    46021 tggacgaagg tgataaccag caagagcgtt tcgccagcta tctcattgcc gccgtgcaac
    46081 aggcaaccaa cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg caatatgcca
    46141 gcctgacgtc actcttcgcc cagcttttca ttgagctggc ggaatggcat agcccacttt
    46201 atctggtcat cgatgactat catctgatca ctaatccagt gatccacgag tcaatgcgct
    46261 tctttattcg ccatcaacca gaaaatctca ccctggtggt gttgtcacgt aaccttccgc
    46321 aactgggcat tgccaatctg cgtgttcgtg atcaactgct ggaaattggc agtcagcaac
    46381 tggcatttac ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca tcgccgattg
    46441 aagccgcaga aagcagtcgg atttgcgatg acgtttctgg ttgggcgacg gcactacagc
    46501 taatcgccct ctccgcccgg cagaataccc actcagccca taagtcggca cgccgcctgg
    46561 cgggaatcaa tgccagccat ctttcggatt atctggtcga tgaggttttg gataacgtcg
    46621 atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg aacgatgcac
    46681 tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg cctcgaagag attgagcgtc
    46741 aggggctgtt tttacagcgg atggatgata ccggcgagtg gttctgctat cacccgctgt
    46801 ttggtaactt cctgcgccag cgctgccagt gggaactggc ggcggagctg ccggaaatcc
    46861 accgtgccgc cgcagaaagc tggatggccc agggattccc cagcgaagcg attcatcatg
    46921 cgctggcggc aggcgatgcg ctgatgctgc gcgatattct gcttaatcac gcctggagtt
    46981 tgttcaacca tagcgaactg tcgctgctgg aagagtcgct taaggccctg ccgtgggaca
    47041 gtttgctgga aaatccgcag ttggtgttat tgcaggcgtg gctgatgcaa agccaacatc
    47101 gctacggcga agttaacacc ctgctggccc gtgctgaaca tgaaatcaag gacatcagag
    47161 aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt aacgatggta
    47221 atccggatga agcggaacgg ctggcaaaac tggcactgga agagctaccg ccgggctggt
    47281 tctatagccg cattgtggca acctcggtgc tgggtgaagt gctgcactgc aaaggcgaat
    47341 tgacccgctc actggcgcta atgcagcaaa ccgaacagat ggcacgccag cacgacgtct
    47401 ggcactacgc cttatggagt ttaattcagc aaagtgaaat tctgtttgcc caagggttcc
    47461 tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct gatcaacgag cagcatctgg
    47521 aacagttgcc gatgcatgag tttctggtgc gcatccgagc acaactgtta tgggcctggg
    47581 cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat tgaagtctta tcgtcttatc
    47641 agccacagca acagcttcag tgcctggcaa tgttgattca atgctcgctg gcccgtggtg
    47701 atttagataa cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg aatggcaaat
    47761 atcacagcga ctggatctct aacgccaaca aagtccgggt gatttactgg caaatgaccg
    47821 gcgataaagc cgccgctgcc aactggttgc gtcatacggc taaaccggag tttgcgaaca
    47881 accacttcct gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg ctgggcgagt
    47941 ttgaacctgc ggaaattgtc ctcgaagaac tcaatgaaaa tgcccgcagt ctgcggttga
    48001 tgagcgatct caaccgtaac ctgttgctgc taaatcaact gtactggcag gccggacgta
    48061 aaagtgacgc ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc accggattta
    48121 tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag ctgattcaac
    48181 tcaatacgct gccggaactg gaacagcatc gcgcacagcg tattctacga gaaatcaatc
    48241 aacatcatcg gcataaattc gcccatttcg atgagaattt cgttgaacgt ctgctaaatc
    48301 atccggaagt gccagaactg atccgcacca gcccgttgac ccaacgtgaa tggcaggtcc
    48361 tggggctgat ttactccggt tacagcaacg aacaaatagc gggtgaactg gaagtggcgg
    48421 caaccaccat caagacgcat atccgcaatc tgtaccaaaa actcggcgtc gcccaccgtc
    48481 aggctgccgt gcagcacgcg cagaaactgc tgaagatgat gggttatggg gtgtgagttt
    48541 gtatagcgtt tttatgtaca ctctttatac tgtatataaa atcgcactac aggctcatga
    48601 atatgaaacc ggaaagcaaa gaagcgccaa tcaatatcag agcgaaagcc tcacagcgtg
    48661 acctgattga tatggcagcg aatctggtcg caaaatcgcg cactgatttt atgctggatg
    48721 ccgcatgccg tgaagcgcaa gacatcttat tagatcaacg actattcatc cttgatgacg
    48781 agcaatacga tgcgtttctc gctgccctgg acgcccccat cactgctgag cgtcaggcca
    48841 aaattaacgc gttgatgaat cgtaaatcgc catgggaata acggctccta ctcccctaac
    48901 gtctgaacat aatctggctg acttttgctg ttccgatcac gggatgaatg aatggctaaa
    48961 aaagaaagcg ttaaaaaatc acagctccgg tctgtctcgt gtttacgtta tctgtattgc
    49021 caatacccgc caggtaatcg gctattactg cctttctaca ggcagtattc aacgtaattt
    49081 ggcccctgga gccatgcgac gtaatgctcc cgaatcgttg cctgttgtcg tattaggcag
    49141 actggcaatt gatcaagcct gggcaggtaa agggttgggc gtcgcattgt tgaaagatgc
    49201 agtctatcgc acaatgtcca tagctcaaca ggtaggggtt cgtgcgctca ttgttcatgc
    49261 cttagacgat tcagtacgca atttctatct gaagtatgcc tttgtacctt caccattcca
    49321 gtcacttacc ttactgtatc ccattactct ggagtaattt acctctaccg gatagtcaca
    49381 ctgatgagtc tgatccggga gggatttcaa tcagtgagtt tggttatcat cacccgcgtt
    49441 acgccatctg tttcagccag agtaaaccgc accggcaaga aacgctccac cacggcgata
    49501 ttggtcagca gatggcatga gggatgggcg accgtaaatt gtcccgcgcc cgccagcgcc
    49561 attggcaaca ctagctggtc agcaagatat tcccccactg ccgccgggct tgccaggtag
    49621 cgtttcacct ctttcaccaa ctgtgccgca accacctcgg cactgacgcg cttttcaccg
    49681 acgacaaaaa agcgttcggt gatattttca ctttcgactt caagcgagac ggtattaccc
    49741 ggcccctggt cgcgcggcag gttatgaata ttctgctcat gcagggaaaa acttgccgcc
    49801 agcgtagcga tttcacgctc agcgacatgc cgcggcacgc cagctaataa cacctctcca
    49861 cgcatctgca caatgttccc gcgctcgcca agttgcaagg tgttaaacga tgccaccggc
    49921 gagacttccg ttgccaccac gccgcctccg gcaggataaa aaccgtgacg taacagcgtg
    49981 gtttgctgat gaattcctat tttcgccagc agcggctcca acacccggcg gataaaatcc
    50041 gcaggcgggg ccgacgggtt atcggtaccg ccgctcactt caacacgcga aggtccatcg
    50101 gcaaaccaca gcgcgggcag caccgtttgc agcaccagcg tacaacttcc tgcgctaccg
    50161 atagcaaagc gataatcgcc gccacgcacg gtgccgggcc ggaagaccag acgctgcgac
    50221 cccagctccg cgccttccac cgtcgccccg caaatttccg tcgccgcctt taccgccgtc
    50281 agatgctggc gcaacagtcc cggttttgcc cgcccggcac gaatgccggt gatggtaaat
    50341 ggctggccgg ttatcatcga cagactcagc gccgagcgca tgatctgccc gccgccttcg
    50401 ccctgtgcgc catccagcgc aatcatcctt ttcatcattt atccttttac gcacaccacc
    50461 tgacgtaggg tgtagataac ttccaccaga tcgctttgtg ccgccatcac cgcatcaata
    50521 tctttatacg ccatcgggat ttcgtcgatc acttcggcat ctttacggca ttccacatgc
    50581 acggtggcac gaatttgatc ttccacgctg aacatttttt tcgctttagt acggctcata
    50641 acccgcccgg caccgtggct gcacgaacag aacgactctt catttcccag cccacggacg
    50701 ataaagcttt tcgctcccat cgaccctgga ataattccat attgaccagc acgcgcagac
    50761 accgcgcctt tacgcgtcac gtagatctct tcaccaaagt gctgttcttt ttgcacatag
    50821 ttgtggtgac agttgatctc ttccatcgcc agcgtttgtg gctgtctgac cgttttctgc
    50881 gtaatgctct gcaacgccgt tactacgttt tccatcatca catcgcggtt aaggctggca
    50941 aaaagctgcg cccaggccac ggctttcagg taatcatcaa agtattccgt accttccata
    51001 aagtacgcca gatcacgcga cggcaatgtt tcaagcgttt cctgcatctc tttttgcgcc
    51061 agatcgataa agtaagtccc gatggcatta ccaattccgc gtgaaccgga gtgcagcata
    51121 atccacacct ggtccgactc atcaaggcag atttcaataa agtggttacc cgttcccagc
    51181 gttcccaggt gtttatagtt gttagtgtta aggaaacgcg gatatttttg cgttaaccac
    51241 tgataaccgg cctccagctc agcccatttc gcatcgacgt taacaggcgg gttttcccat
    51301 gcgcctttat cacgtttaca acggccagtg gtacgcccgt gcggcaccgc agtttcaatc
    51361 gcctgacgca gctccgccag gttttccggc aggtcagccg ccgttaaagc ggtacgtagt
    51421 gcgttcattc cacagccaat atccacgccc accgccgccg gaataatcgc ccctttggtc
    51481 gggatcacgc taccaatggt ggaaccttta cccaggtgta catcaggcat taccgcaata
    51541 tgtttgaaaa taaacggcat cttcgccgta ttaataagtt gctgacgcgc atcggcttct
    51601 accggcacgc ctttggtcca catttttacc ggggcatttt cagtggtcag taattcgtaa
    51661 ttcattttgt ttttctcttt tcgttgtttg cg
//
LOCUS       DAZKFZ010000009        51295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000009 DAZKFZ010000000
VERSION     DAZKFZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..285
                     /locus_tag="ACHOQW_000513"
     CDS             <1..285
                     /locus_tag="ACHOQW_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9571644.1"
                     /translation="RSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPRKNI
                     RQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPH"
     gene            547..2169
                     /gene="yhiJ"
                     /locus_tag="ACHOQW_000514"
     CDS             547..2169
                     /gene="yhiJ"
                     /locus_tag="ACHOQW_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9571645.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISILNRRDIILESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2359..2472
                     /gene="yhiY"
                     /locus_tag="ACHOQW_000515"
     CDS             2359..2472
                     /gene="yhiY"
                     /locus_tag="ACHOQW_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP9571646.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2535..3602
                     /gene="yhiI"
                     /locus_tag="ACHOQW_000516"
     CDS             2535..3602
                     /gene="yhiI"
                     /locus_tag="ACHOQW_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9571647.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3599..6334
                     /gene="rbbA"
                     /locus_tag="ACHOQW_000517"
     CDS             3599..6334
                     /gene="rbbA"
                     /locus_tag="ACHOQW_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP9571648.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6334..7458
                     /gene="yhhJ"
                     /locus_tag="ACHOQW_000518"
     CDS             6334..7458
                     /gene="yhhJ"
                     /locus_tag="ACHOQW_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9571649.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7531..7806
                     /locus_tag="ACHOQW_000519"
     CDS             7531..7806
                     /locus_tag="ACHOQW_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9571650.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7803..8162
                     /locus_tag="ACHOQW_000520"
     CDS             7803..8162
                     /locus_tag="ACHOQW_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9571651.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8212..9072)
                     /locus_tag="ACHOQW_000521"
     CDS             complement(8212..9072)
                     /locus_tag="ACHOQW_000521"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFP9571652.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(9104..9373)
                     /locus_tag="ACHOQW_000522"
     CDS             complement(9104..9373)
                     /locus_tag="ACHOQW_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP9571653.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(9363..10871)
                     /locus_tag="ACHOQW_000523"
     CDS             complement(9363..10871)
                     /locus_tag="ACHOQW_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9571654.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(10864..12222)
                     /locus_tag="ACHOQW_000524"
     CDS             complement(10864..12222)
                     /locus_tag="ACHOQW_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9571655.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(12299..12580)
                     /locus_tag="ACHOQW_000525"
     CDS             complement(12299..12580)
                     /locus_tag="ACHOQW_000525"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9571656.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(12577..13050)
                     /locus_tag="ACHOQW_000526"
     CDS             complement(12577..13050)
                     /locus_tag="ACHOQW_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9571657.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(13075..13821)
                     /locus_tag="ACHOQW_000527"
     CDS             complement(13075..13821)
                     /locus_tag="ACHOQW_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9571658.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(14020..14421)
                     /gene="nikR"
                     /locus_tag="ACHOQW_000528"
     CDS             complement(14020..14421)
                     /gene="nikR"
                     /locus_tag="ACHOQW_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP9571659.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(14427..15233)
                     /gene="nikE"
                     /locus_tag="ACHOQW_000529"
     CDS             complement(14427..15233)
                     /gene="nikE"
                     /locus_tag="ACHOQW_000529"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP9571660.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(15230..15994)
                     /gene="nikD"
                     /locus_tag="ACHOQW_000530"
     CDS             complement(15230..15994)
                     /gene="nikD"
                     /locus_tag="ACHOQW_000530"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP9571661.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(15994..16827)
                     /gene="nikC"
                     /locus_tag="ACHOQW_000531"
     CDS             complement(15994..16827)
                     /gene="nikC"
                     /locus_tag="ACHOQW_000531"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP9571662.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(16824..17768)
                     /gene="nikB"
                     /locus_tag="ACHOQW_000532"
     CDS             complement(16824..17768)
                     /gene="nikB"
                     /locus_tag="ACHOQW_000532"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP9571663.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(17768..19342)
                     /gene="nikA"
                     /locus_tag="ACHOQW_000533"
     CDS             complement(17768..19342)
                     /gene="nikA"
                     /locus_tag="ACHOQW_000533"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9571664.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(19453..20040)
                     /gene="acpT"
                     /locus_tag="ACHOQW_000534"
     CDS             complement(19453..20040)
                     /gene="acpT"
                     /locus_tag="ACHOQW_000534"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP9571665.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(20042..21271)
                     /locus_tag="ACHOQW_000535"
     CDS             complement(20042..21271)
                     /locus_tag="ACHOQW_000535"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFP9571666.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(21268..21999)
                     /locus_tag="ACHOQW_000536"
     CDS             complement(21268..21999)
                     /locus_tag="ACHOQW_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFP9571667.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(21999..22463)
                     /locus_tag="ACHOQW_000537"
     CDS             complement(21999..22463)
                     /locus_tag="ACHOQW_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFP9571668.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(22460..23629)
                     /locus_tag="ACHOQW_000538"
     CDS             complement(22460..23629)
                     /locus_tag="ACHOQW_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFP9571669.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(23631..24215)
                     /locus_tag="ACHOQW_000539"
     CDS             complement(23631..24215)
                     /locus_tag="ACHOQW_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFP9571670.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(24212..26530)
                     /locus_tag="ACHOQW_000540"
     CDS             complement(24212..26530)
                     /locus_tag="ACHOQW_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFP9571671.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(26499..27104)
                     /locus_tag="ACHOQW_000541"
     CDS             complement(26499..27104)
                     /locus_tag="ACHOQW_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFP9571672.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(27101..27523)
                     /locus_tag="ACHOQW_000542"
     CDS             complement(27101..27523)
                     /locus_tag="ACHOQW_000542"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9571673.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(27527..29203)
                     /locus_tag="ACHOQW_000543"
     CDS             complement(27527..29203)
                     /locus_tag="ACHOQW_000543"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9571674.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(29194..29547)
                     /locus_tag="ACHOQW_000544"
     CDS             complement(29194..29547)
                     /locus_tag="ACHOQW_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFP9571675.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(29534..30895)
                     /locus_tag="ACHOQW_000545"
     CDS             complement(29534..30895)
                     /locus_tag="ACHOQW_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HFP9571676.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRAIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(30892..31473)
                     /locus_tag="ACHOQW_000546"
     CDS             complement(30892..31473)
                     /locus_tag="ACHOQW_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9571677.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(31478..31729)
                     /locus_tag="ACHOQW_000547"
     CDS             complement(31478..31729)
                     /locus_tag="ACHOQW_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9571678.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(31741..31998)
                     /locus_tag="ACHOQW_000548"
     CDS             complement(31741..31998)
                     /locus_tag="ACHOQW_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFP9571679.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(31973..32794)
                     /locus_tag="ACHOQW_000549"
     CDS             complement(31973..32794)
                     /locus_tag="ACHOQW_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9571680.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(32791..33513)
                     /locus_tag="ACHOQW_000550"
     CDS             complement(32791..33513)
                     /locus_tag="ACHOQW_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFP9571681.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(33554..34612)
                     /locus_tag="ACHOQW_000551"
     CDS             complement(33554..34612)
                     /locus_tag="ACHOQW_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP9571682.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(34681..35058)
                     /locus_tag="ACHOQW_000552"
     CDS             complement(34681..35058)
                     /locus_tag="ACHOQW_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9571683.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(35450..36499)
                     /gene="yhhT"
                     /locus_tag="ACHOQW_000553"
     CDS             complement(35450..36499)
                     /gene="yhhT"
                     /locus_tag="ACHOQW_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9571684.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            36631..37848
                     /gene="yhhS"
                     /locus_tag="ACHOQW_000554"
     CDS             36631..37848
                     /gene="yhhS"
                     /locus_tag="ACHOQW_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9571685.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(37852..38409)
                     /gene="dcrB"
                     /locus_tag="ACHOQW_000555"
     CDS             complement(37852..38409)
                     /gene="dcrB"
                     /locus_tag="ACHOQW_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP9571686.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(38482..39147)
                     /gene="yhhQ"
                     /locus_tag="ACHOQW_000556"
     CDS             complement(38482..39147)
                     /gene="yhhQ"
                     /locus_tag="ACHOQW_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP9571687.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            39368..39613
                     /gene="tusA"
                     /locus_tag="ACHOQW_000557"
     CDS             39368..39613
                     /gene="tusA"
                     /locus_tag="ACHOQW_000557"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP9571688.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(39715..41913)
                     /gene="zntA"
                     /locus_tag="ACHOQW_000558"
     CDS             complement(39715..41913)
                     /gene="zntA"
                     /locus_tag="ACHOQW_000558"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP9571689.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41987..42613)
                     /gene="yhhN"
                     /locus_tag="ACHOQW_000559"
     CDS             complement(41987..42613)
                     /gene="yhhN"
                     /locus_tag="ACHOQW_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP9571690.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            42754..43113
                     /gene="yhhM"
                     /locus_tag="ACHOQW_000560"
     CDS             42754..43113
                     /gene="yhhM"
                     /locus_tag="ACHOQW_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP9571691.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(43116..43385)
                     /gene="yhhL"
                     /locus_tag="ACHOQW_000561"
     CDS             complement(43116..43385)
                     /gene="yhhL"
                     /locus_tag="ACHOQW_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP9571692.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(43375..43971)
                     /gene="rsmD"
                     /locus_tag="ACHOQW_000562"
     CDS             complement(43375..43971)
                     /gene="rsmD"
                     /locus_tag="ACHOQW_000562"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP9571693.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            44121..45617
                     /gene="ftsY"
                     /locus_tag="ACHOQW_000563"
     CDS             44121..45617
                     /gene="ftsY"
                     /locus_tag="ACHOQW_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9571694.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            45620..46288
                     /gene="ftsE"
                     /locus_tag="ACHOQW_000564"
     CDS             45620..46288
                     /gene="ftsE"
                     /locus_tag="ACHOQW_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9571695.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            46281..47339
                     /gene="ftsX"
                     /locus_tag="ACHOQW_000565"
     CDS             46281..47339
                     /gene="ftsX"
                     /locus_tag="ACHOQW_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9571696.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            47584..48438
                     /gene="rpoH"
                     /locus_tag="ACHOQW_000566"
     CDS             47584..48438
                     /gene="rpoH"
                     /locus_tag="ACHOQW_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP9571697.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            48709..49812
                     /gene="livJ"
                     /locus_tag="ACHOQW_000567"
     CDS             48709..49812
                     /gene="livJ"
                     /locus_tag="ACHOQW_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP9571698.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            49962..50228
                     /gene="ataR"
                     /locus_tag="ACHOQW_000568"
     CDS             49962..50228
                     /gene="ataR"
                     /locus_tag="ACHOQW_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFP9571699.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            50235..50762
                     /gene="ataT"
                     /locus_tag="ACHOQW_000569"
     CDS             50235..50762
                     /gene="ataT"
                     /locus_tag="ACHOQW_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFP9571700.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(50759..51142)
                     /gene="panM"
                     /locus_tag="ACHOQW_000570"
     CDS             complement(50759..51142)
                     /gene="panM"
                     /locus_tag="ACHOQW_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP9571701.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT        12321 a        13324 c        13816 g        11834 t
ORIGIN      
        1 agaagcaata ctatggcacc cacacctgta tatgctgatt atttaaataa cactactgat
       61 ttcagaccag atcctgaaga tatcttcaaa tgtggacaga cgctcgaatg tggcgacccc
      121 cgcaaaaaca ttagacaaaa atatggccat catgggttag gggtaaatca gaatcaacaa
      181 tttgataatg gtgttatggg gctaaattcc aggatggcag tgtttgatga aaataaaaaa
      241 ataataggtt cttcctcagg actaagttgt tttcaaccac attaaaactc tatgagtgct
      301 gtggtatatc tttttaatcc tgccagtatt tcatagtttt tcttaatcga ttgtagaggc
      361 atgtactgat gacgcctcta caatctttag ggactttctc tactatgaga taagaaattt
      421 catattatgc tttaaggctg aatgatttgt gcaaaatata attatttttc catcgaacat
      481 gaggtgtatg tcataagatt aaatgataac tatgtattcc tcgcggtgaa ttatatgagg
      541 taaaagatga aaattggaac tgtggcaggt actaacgaca gtacaacgac aatcgcaaca
      601 aatgatatgg tgcaagagca tgttactaac ttcactaaag aactgtttgg gtatattgca
      661 aatggtatag gtgatgatat atcaagtatt gccaggacaa tgctcggtga ggttctggaa
      721 aaagtagatg attggcaaat agaacggttc caacaatcta ttcaagatga caaaatttca
      781 tttaccatac aaacggatta ttcagaaaaa tatagtatgc tatcgggcat gcgtgctcat
      841 attctacgta ggaataacca ttatcagttt attgtgacga ttaatagcaa aaattacggt
      901 tgccctcttg ataataccga tattaattgg tgcggcattg tttatttact taataatatg
      961 acagtaaatg ataatgctaa tgatgttgca gttactgagt cgtacaaacc catctggaac
     1021 tggaaaatat cgcaatacaa tgtatctgat attaaattcg aaacaattat aaaaccccag
     1081 ttcgctgaca gaatctattt ctccaattgt tcacctgttg atcccacaag taccagacca
     1141 acctattttg gtgataccga tggctcagtt ggtgcagttt tgtacgcgct ttttgcaacg
     1201 ggacatttgg gaatcatggc tgaaggagaa aatttcctta gccagttgct taatatagaa
     1261 gatgaagttc tcaatgtatt gctaagggag aactttaatg agcaagttga tactaatgtt
     1321 aatacaataa tcagtatcct gaacagaaga gacattattt tagaaagtct tcagccttat
     1381 ttagttatta ataaagacgc agtgacgcca tgtacgtttt tgggtgacca aaccggcgat
     1441 cgatttagta atatctgtgg cgatcaattt atcatcgatt tgttaaaacg cataatgagc
     1501 atcaatgata acgttcatgt attagctgga aatcatgaaa cgaattgcaa tggtaactat
     1561 atgcagaact tcacgcgtat gaaaccactt gatgaggata cctacgacgg gataaaagat
     1621 tatcctgtat gtttctatga ctccaaatac aaaataatgg caaatcatca tgggataact
     1681 tttgatgatc agcgaaaacg ttacattata ggaccgataa cggtctctat tgatgaaatg
     1741 accaatgctc tcgatccggt cgaattagcg gcaattatta ataaaaagca tcatactata
     1801 attaatagta aaaagtttaa aactagccgg gctatctctt gtcgatcctt taatcgttat
     1861 ttttctgttt cgacagatta cagacctaaa cttgaagcac tattagcatg ctcacaaatg
     1921 ttaggtataa atcaagttgt tgcacataac ggtaatggtg gaagagaatg cataggtgaa
     1981 acggggacag ttttaggtct taacgcccgt gacagtaaac atacgggaag aatgttcagc
     2041 atgcataatt gccaaattaa ccctagtgcg gggcctgaga taaccactcc ctggaagtct
     2101 taccaacatg aaaaaaacag aaatggactt atgcctttga ttagaaggcg cacaatgtta
     2161 cagctctgaa ccatagacaa catgaatatt aatgttacta tctggttgag actgttgata
     2221 aaacgtaata aggaatgctc tgttcctgca actgaacagg cgtgaaacgc tatccaacag
     2281 gatggatacc gttttgtcag ccagttttat agatcacact tatgagtatg cgtgtttcat
     2341 ttacggccag gaatgctgat gatgacaaca ttactacccg ttttcactaa gccttccccg
     2401 ttggcgctca atgctctgcg cgctggtcgt atttgtcgtt tccttcttat cccggatgga
     2461 agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc gtcgtttgct tacagggatt
     2521 atggcgggtc gattatggat aagagtaagc gccatctggc gtggtggggt gtcgggttac
     2581 tggcggtggc tgctatcgta gcgtggtggc tgttgcgccc ggcaggtgtg ccggaaggct
     2641 ttgccgtcag caatgggcgc attgaagcga cggaagtgga tatcgccagc aaaattgccg
     2701 ggcgtatcga caccattctg gtgaaagaag gccagtttgt tcgcgaaggc gaagtgctgg
     2761 cgaagatgga tactcgcgtg ttgcaggagc agcgactgga agccatcgcg caaatcaaag
     2821 aggcacaaag cgccgttgcc gccgcgcagg ctttgctgga gcaacgacaa agcgaaactc
     2881 gcgccgcaca gtcgctggtt aatcaacgcc aggccgaact ggactccgtt gccaaacgtc
     2941 atacgcgttc ccgctcgctc gcccaacgag gggctatttc tgcgcaacag ctggatgacg
     3001 atcgcgccgc cgctgagagc gcccgagctg cgctggaatc agcaaaagcc caggtttcgg
     3061 cttctaaagc cgctatagaa gcggcacgca ccaatatcat tcaggcgcaa acccgcgtcg
     3121 aagccgcaca agccactgaa cggcgcattg ccgcagatat cgatgacagc gaactgaaag
     3181 ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc aggggaagtg ctggcggcag
     3241 gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta tatgaccttc ttcctgccaa
     3301 ccgaacaggc gggcacgctg aaacttggcg gtgaagcccg gctgatcctc gatgccgcgc
     3361 cagatctgcg tattcctgca accatcagtt ttgtcgccag tgtcgcccag ttcacgccaa
     3421 aaaccgtcga aaccagcgat gaacggctga aactgatgtt ccgcgtcaaa gcgcgtatcc
     3481 caccggaatt actccagcag catctggaat atgtcaaaac gggcttgccg ggcgtagcgt
     3541 gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct cgtggtgagg ttgccgcaat
     3601 gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa ctggcgggcg tgagccagca
     3661 ttatggaaaa accgttgcgc tgaacaatat cacactcgat attccggccc gctgtatggt
     3721 cgggctgatt ggtccggatg gcgtcgggaa gtcgagttta ttgtcattga tttccggtgc
     3781 tcgtgtcatt gagcagggca atgtgatcgt gctgggcggc gatatgcgcg acccgaagca
     3841 tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag gggctgggca aaaacctcta
     3901 ccacaccttg tcggtgtatg aaaacgtcga ttttttcgct cgcctgttcg gtcacgacaa
     3961 agcggagcgg gaagtgcgaa tcaatgagct gctgaccagc accgggttag caccgtttcg
     4021 cgatcgtccg gcagggaaac tctccggcgg gatgaagcaa aaactggggc tgtgctgtgc
     4081 gttaatccac gacccggaac tgttgattct tgatgagcct acgacggggg ttgacccgct
     4141 ctcccgcgcc cagttctggg atctgatcga cagtattcgc cagcggcaga gcaatatgag
     4201 cgttctggtc gccacggcct atatggaaga ggcggaacgc ttcgactggt tggtggcgat
     4261 gaatgccgga gaagtgttgg caactggcag cgccgaagag ctgcggcagc aaacgcaaag
     4321 cgccacgctg gaagaagcat ttataaatct gttaccgcaa gcgcaacgcc aggcgcatca
     4381 ggcggtagtg atcccaccat atcaacctga aaacgcagag attgccatcg aagcgcgcga
     4441 tctgaccatg cgttttggtt ccttcgttgc cgttgatcac gtcaatttcc gcattccacg
     4501 cggggagatt tttggttttc ttggttcgaa cggctgcggt aaatccacca ccatgaaaat
     4561 gcttaccgga ttgctgcccg ccagcgaagg tgaggcgtgg ctgttcgggc aaccggttga
     4621 tccaaaagat atcgatactc gccgtcgggt gggctatatg tcgcaggcgt tttcgctcta
     4681 taacgagctc accgtgcggc aaaaccttga gttacatgcc cgtttgtttc acatcccgga
     4741 agcggaaatt cccgcaagag tggctgaaat gagcgagcgt tttaagctca acgacgttga
     4801 agatgttctg ccggagtcat tgccgctcgg cattcgccag cggctttcgc tggcggtggc
     4861 ggtgattcat cgcccggaaa tgttaatcct cgatgaacct acttctggcg tcgatccggt
     4921 ggcgagggat atgttctggc agttgatggt cgatctctcg cgccaggaca aagtgactat
     4981 cttcatctcc acccacttta tgaacgaagc ggaacgctgc gaccgtatct ctctgatgca
     5041 cgccggaaaa gtgctcgcca gcggtacacc gcaggaactg gttgagaaac gcggagccgc
     5101 cagtctggaa gaggcattta tcgcctattt gcaggaagcg gcaggacaga gcaacgaagc
     5161 cgaagcgcct cccgtgatac acgacaccac acacgcgccg cgccagggat ttagcctgcg
     5221 ccgtctgttt agctacagcc gccgcgaagc gctggaactg cgacgcgatc cggtacgttc
     5281 gacgctggcg ctgatgggaa cagtgatcct gatgctgata atgggttacg gcatcagtat
     5341 ggatgtggaa aacctgcgct ttgcggtgct tgaccgcgac cagaccgtca gtagccaggc
     5401 gtggacgctc aatctctccg gttcccgtta ctttatcgaa cagccgctgc ttaccagtta
     5461 tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg gtggcaattg agatcccgcc
     5521 caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa ctcggtgtct ggatcgacgg
     5581 tgcgatgccg agccgcgccg aaactgtaaa aggttacgtg caggccatgc accagagctg
     5641 gctacaggat gtggcgagcc gacaatccac accctccagc caaagcgggc tgatgaatat
     5701 tgagacgcgt tatcgctata acccggacgt aaaaagcctg ccagcgattg ttccggcggt
     5761 gatcccgctt ctgctgatga tgatcccgtc aatgctaagc gcccttagcg tggtgcggga
     5821 aaaagagcta gggtcgatta tcaaccttta cgtgaccccc accacccgta gcgaattttt
     5881 gcttggcaaa cagctgccgt acatcgcgct ggggatgctg aactttttcc tgctctgcgg
     5941 cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt ttcctgacgc tcaccctggc
     6001 ggcgctgctg tatatcatca tcgccaccgg aatggggctg ctgatctcca cctttatgaa
     6061 aagccagatt gccgccattt tcggtacggc gattatcacg ttgatcccgg cgacacagtt
     6121 ttccgggatg atcgatccgg tagcttcgct ggaagggccg ggacgttgga tcggcgaggt
     6181 ttacccgacc agtcattttc tgactatcgc ccgcgggacg ttctcgaaag cgctggatct
     6241 gactgatttg tggcaacttt ttatcccgtt gctgatagcc ataccgctgg tgatgggctt
     6301 aagcatcctg ctgctgaaaa aacaggaggg atgatgcgcc atttacgcaa tatttttaat
     6361 ctgggtatca aagagttgcg cagtctactc ggtgataaag cgatgctgac attgattgtc
     6421 ttctcgttta cggtgtcggt gtattcgtca gcgaccgtca cgccaggatc gttgaacctg
     6481 gcaccgatcg ccattgccga tatggatcaa tcgcagttat cgaaccggat cgttaacagc
     6541 ttctatcgcc cgtggttttt gccaccggag atgatcaccg ccgatgagat ggatgccgga
     6601 ctggacgccg ggcgttatac ctttgcgata aatattccgc ctaattttca gcgtgatgtc
     6661 ctcgccggac gccagccaga tattcaggtg aacgtcgatg ccacgcgcat gagccaggca
     6721 tttaccggca atgggtatat ccagaatatt atcaacggtg aagtgaacag ctttgtcgcg
     6781 cgctaccgtg ataacagcga accgttggta tcgctggaaa cccggatgcg ctttaacccg
     6841 aaccttgatc ccgcgtggtt tggcggggtg atggcgatca tcaacaacat taccatgctg
     6901 gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg aacacggcac ggtggaacac
     6961 ttactggtga tgccgataac gccgtttgag atcatgatgg cgaagatctg gtcgatgggg
     7021 ttggtggtgc tggtggtatc gggattatca ctggtgctga tggtaaaagg cgtgttgggc
     7081 gtaccgattg aaggctcgat cccgctgttt atgctgggcg tggcgcttag tctgtttgcc
     7141 accacgtcaa tcggcatttt tatggggaca atagcgcgtt caatgccgca actggggctg
     7201 ctggtgattc tggtgctgct gccgctgcaa atgctttccg gtggttccac gccgcgcgaa
     7261 agtatgccgc agatggtgca ggacattatg ctgatcatgc cgacgacaca ctttgttagc
     7321 ctcgcgcagg ccattctcta ccgtggtgcc ggattcgaaa tcgtctggcc gcagttcctg
     7381 acgctgatgg caattggcgg cgcgtttttc accattgcgc tgctgcgatt caggaagacg
     7441 attgggacaa tggcgtaagg aagggaaact tgacttcata ctgtgatgtc atactattga
     7501 tgtcataaag tgaagtcata aggacatgcc atgagaacca aggtacaggc tttacggaag
     7561 aaacaaaaaa atacattgga tcaaatattt aaaactcctg ttcctcaagg gatcaagtgg
     7621 tctgatatag agtcactggt taaagcatta ggcggagaaa ttaaggaagg aagaggttcg
     7681 cgttgtaagt tcatactaaa tatgagcgtt gcgtgtttcc atcggcctca tccgtcgcca
     7741 gataccgata aaggcgctgt agaaagcgtg cgtgactggt tactaagtat aggggtaaaa
     7801 ccatgatcaa actgaagacg ccaaactcta tggagattgc cgggcaacct gctgttatca
     7861 cttacgtgcc agaactgaat gcatttcgtg gcaaattcct gggcttgtca ggttattgtg
     7921 attttgtctc tgacagcatt caggggctgc aaaaagaagg ggaactttcc ctgcgagagt
     7981 atcttgagga ttgtaaagct gcgggtattg agccgtatgc gcgtacagaa aagattaaga
     8041 cctttacgtt acgctatcca gaatcgctga gtgaacgcct taataatgcg gctgcgcagc
     8101 aacaagtctc tgttaatacc tatattattg aaacactgaa tgagcgtttg aatcatttat
     8161 aacttatagc ggcccaacaa tttctgttgc gccgctatat ttttgtggct cttaagatgc
     8221 ctttctaacc aaccgatgcc cctcattcat catcaggatt ttgctataca catcttcctc
     8281 gacggcgttt tttgctgatg ccatgactcg tgccagattc gcctcgttgt gattattcac
     8341 atacgctttg ccgacggcgg tgataaacgc tttgcgcaga tcgctggcga tatttacttt
     8401 agcgacgcga taattaacaa agctgcgtaa tatctccggg gcgataccgg agccgccgtg
     8461 aataaccaac ggcaccggac agacttcggc aatgcgcttc agcaggggga tatcgatacg
     8521 cggtatatcg tccaggccat gaacgttgcc aatagaaact gccagcatgt cgcaaccggt
     8581 acgctcgaca aaggtcttca ccttttccgg ttccgttttg caatcggctt cgctgacgtg
     8641 atcatcttct ttgccgagaa tcgcccccag ctccgcttca accggtacgc cataggattt
     8701 acagaaatcg acagcttcct gggtgaaagc gatattctct tcaaaagaga atgcggcacc
     8761 gtcaatcatg accgaggtaa agcccgcgcg caccgcctgt ttaacatcct cctgggtttt
     8821 cccgtgatcc agatgaagac tgaccggaac atccatttta tcggcgtgac gtttcacgat
     8881 ttcataaatg taatcgtatc ccgagagttg tgcattggtt ggcgcaatct gaatgaagtt
     8941 gggtaatccc gaacgctcaa tagcgtttaa aattgaaatt gtcgtctcca gattggtggt
     9001 attaaatgcg cccgctaata cccgtttttc ctgaatgcgg tcgagcagta tccgtccatt
     9061 aactaatggc atattgaaaa cctcatcttt taatatcagt taattattca gcttcaccga
     9121 agcggtcgcg aaataagtcg gtgagagtct gtatggcctc gttttcatca tcaccgtcgc
     9181 agcgtatttc caccgttttt ccgcctttta ttccggcgcg cataatattc atcatgctgg
     9241 tggcattaat atcattaccc tcatagtgca gcgtcacttt acagctatat tttttggcgg
     9301 ctttcgccag acaggccgcc ggacgtgcat gcaggccagt actgtttctg acctccacgg
     9361 ttttattcaa catagtgttc ctctcgatga tgtaatggca tcacgccttc ttgttgtaat
     9421 ttcaactgat accagctttt ccatttgtgc tgataacgct gtaattgatg gtatctcgcc
     9481 cgattcggta acaaacgttg ggctatagcg actggcggcg gtggaatatc cggtgcggcg
     9541 gttaataatg ctccgacaga aaccgcctcg ttaaatgcaa ttgcttcaac cggacgctgt
     9601 aaaatatccg cctttaattg cagccaaaga ggattatggg ttgccggacc gatcactttt
     9661 aacgcgctgg tgccaggcac gttaaaacag gcgaagcagt tggcaaattc catggttaat
     9721 cccagtgata cgctggcaat taacatgtcg atacttaacg tatcgccaag cccataaaat
     9781 aatccacggg tatggcgatt tttatagggc gaacccgaac cgcgaagatg tggaataaag
     9841 acgggaatgt catcgagcaa ccaattcccc gccagataat cagcatgtcc gcgagtaagt
     9901 gatgcggcga tctcctcatc ggttagccgg aacgtgttgc gcagccactc cagcgcgaaa
     9961 ccgcccacgg gcagcgacgc aaacagggtg aactggttgg caagtgagta acagccgttt
    10021 gccagcttgt cacgtttggc ctgttcatcc agcgtcggtt gtgtatccag ttgcagaatg
    10081 ccttccgtgg tccccgtcga gttaaggata tcgcctggca tcatctgcaa cgctcgcgca
    10141 cccaccatat ggtcatgtcc ggccagcgtc acgctgaccg gttgcgagaa cccaagcgtc
    10201 ttgcaaagtg actcgctgac ccatccggcg tgctcgcctg gctgcaccaa tggcgcaaat
    10261 gccgtacagg gaacgtgtaa caacgccgcc gctttcgctg accattcgcc tttgcgcacg
    10321 tccagacaca gcgtgcggct ggctaaggtg atatccgtgt gctgttcgcc agtcagccgc
    10381 cagagcagca cttccggcgc atgtagccag cgcaggccgc ggcgattacg cagcgggtaa
    10441 tgttccagca accagcgcat tttgaaggca gagtaattgc tgtgtagtgg tagaccgcag
    10501 atgtcataaa gtgccgcact gtctgcctcg ctaagcgttg ccagatactc ttcaccgcga
    10561 cggtcatacc atgccagcat tggcgtcagg atctcgccat gctcgtcaag gaacacgcct
    10621 gattcgccaa aactggcaat gctgatccgt ctgactggca gcggcgaagc ggcgttcagt
    10681 tgcgctatcg cctggcggac ctcctgccag agggcgtcga tatcgaaatc gacatcgccc
    10741 tgtggggaga tctgctttgc cgtcacgaat ttatgcgcgc ccaacgtcgt tgcgtccagg
    10801 caggaaaagc aggtgacttt gcaattggtg gtgccaatat cgataacgat agctgcgctg
    10861 ttatcaggca taaaccgtgc tccgcgtcat tcggaaaatg ccatacagta ccagggcgat
    10921 aaccggcacc agaccaatgg cgatgagatt gccgttgaag atttccagca gccagaagcg
    10981 gaacgggtta ccgccatcaa gaaaactgga aacctggccg ctggagcctt cggcaaattt
    11041 gaagttaacg tctttcgcca ttccggtaat aaacggcgcg attttggtag caatccatag
    11101 gtcggcaata atcaccggga tcgccgccag aacggtgcgg aaaatattgc cacggctggc
    11161 taaagcaatc atcgacgcca ttaccgccag gttggcaagg tcgccaagcg gcaggacttc
    11221 attacccgga aggacaaacg cgataaagac cgagattggc gttagcagca gggcggtgga
    11281 gataatcgcc ggattaccta cggcaacggc gatatccagg ccgatataga gatcgtcacg
    11341 gtcagggtat ttggcattga gatacttttt aatagcttca gacagcggta ataaaccttc
    11401 catcaggatg cgtaccatgc gtggcaggat gaacatcaca ccgccaatgc tgatgccaag
    11461 cagcaatact tttttgaaat cgtatccggc aattacgccg agcagaatgc ccagaatagt
    11521 gccaaccatc atcggttcgc cgaagatgcc aaactttttc tgtacggttt ccgggtcgat
    11581 atgaatacga ttgaggccag ggatatggtc gatgattttg tcgaccagca gaccgaccgg
    11641 gaagaacacc accgaagaga gcgtcggcaa tgagatccct tccagtccaa agtatttttg
    11701 taccagtggc gcagaccagt cggcgagttt aagtactacc accgcagcaa tcgccgccgc
    11761 cagcaaacca aagaacaggc tgccggtgct gtaataaacc aacgtaccag caagggcaaa
    11821 atgccagtag ttccacatat ctacgtcaac ggtacgggtt ttatttaacg ccagcatcgc
    11881 cacgttaatc agaatggtca ggggaataac aaacggggca attggcgaac cccatgtaat
    11941 agcggaaagc ggcggccagc ctaaatctac caccggtaaa ttaattccgg tgcgttcaac
    12001 catcgcctgg gccgctggcc cgacattgct ggtcagaata tcgaaaatgg cataaatacc
    12061 gacaaaacca atgccgactg ttaatgacga acgtaaggct ttagcgggtt ttactttaaa
    12121 acagagagca agaataaata acaccaacgg caacattacc gtcgggccaa atcccaatat
    12181 atattgcaca atattataga gggtatgcgc gatatcgttc ataataaacc tctttttcag
    12241 gcgagagcgc acctcgcgtt agcgcctgat ggagtgggtt gaaattaatt attattaatt
    12301 actttttaag gatagaaaca atttgcgcca gtactttttc ttcgccaata ccggtaataa
    12361 tcggcaagcc gttcaccacc gggacgtcga gttcataagg cactttagtt gttgaaacta
    12421 ccagtgctac gccgtcagta tttccaggta aagaggcaac gttggtttgg gaataattga
    12481 cgttaagacc ttgctctttg caatattcca tgactttttc tgttaccgcc gtggatgtgg
    12541 caatacccgt ggcgcagaca aataaaatgg tttgactcat atttcacctc gttctttcat
    12601 tttgtttaag attactgtgt gtatttctgt tgtttccatc gcaagcaggg cttgcataaa
    12661 gggcgtatcc tgaatcattt ccataatcca gcccagggca tttaattgtt tattgctttc
    12721 gcttaaggcg agcaaaaaaa tcaccctgac aggtacgggg tcaggatcgc cgcccatatc
    12781 ggcaaactct accggttccg gcaggatgcc gacggcaatt gcattgtgcc tgacatgttt
    12841 atggtccgtg tgcggaatag cgacgcacgg tggttcggta ggcagccccg ttgggtacgt
    12901 cgcctcacgt ttcagtacgt gatcggcaaa atcagggacg accagctcgg cggcaaccaa
    12961 cggttgcgct aactggcgga tgatctgttc aacgcttaac cctgccggaa gtcgttgaat
    13021 ataattttcc tgaaaatgta tgccttgcat tttattcctc ttaaaaaagt gtacttaact
    13081 cacttcccgc cgttgcagta cggtgccaag ataatattta tttcctttta accagacgtt
    13141 gccaaactca atgggtaatc ctcgggagaa aaagactaac tgctccagat gcaaaacggg
    13201 tgcgtcttca ctgatatcta aaaaatgacc acgttcatta ccaattaatc gcgcggcata
    13261 gcgactttcc gagtaacgaa tttttctttg cgacaaactt tctattgttg gaaataaatt
    13321 gtgttgatta aaatcgattt cagcaatacc ggggcatagc tcaatattga tacggttctc
    13381 tatcagcatc gctttttcat caccaattga gcgtaagcgt tcaaggtaaa gaatatcctg
    13441 accgggcgtt attcttaatt tctctgccac ataacgatta gccgattcaa tccgtgacgt
    13501 aataacttca gtggtaaaat gtattttctg gctttccagc gattccgcga atgacaataa
    13561 tccttcacct aacggatatg ccacgttctc ttttttgaca aatgttccct taccctggac
    13621 ctgtattaac acgccttctt ttaccagttg ggcgaccgct tttttaacgg ttccccgact
    13681 cacgtccagc atatccataa tctggtattc cgaaggaatg cgatcgtctt ccaccagatc
    13741 tccggtataa atactttctc ttatccactc caccacttgt tgatagagcg gtatttgtga
    13801 cgacttactt aatgatttca tggcgcgcac ctgaacattt acagttgtct atggacaatc
    13861 atggacaact tcaagcgtat agtaggtgaa cgggagaaaa agaggatcga agcgtctcac
    13921 attttgaggg accaaatcgt gatcaaaaaa atgtgacgtg agtcgatatt tgtcagaagt
    13981 ttactgcgcc gtaaggaaat aacggcgcgt tggtaggtgt taatcttcct tcggcaggca
    14041 ctgcaaatgc ccgtgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
    14101 gtcacccatg tcacctttca acacggcgat ttccagacag tcgtcgtggt tgatgtgcac
    14161 atgtagcgtg gcgacggaga ggtcgtggtg atgatgctgg gtggaaacaa tacggctggc
    14221 taagtcgcgt ttttcgtgtt catacacata cgacagcacc gcgaaacctt gtgtgccgtg
    14281 ctgctgggta gcttcctgcg ccagggcgct acgcagaata tcgcggatag cttcggaacg
    14341 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
    14401 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg gcgcacgggg
    14461 aatgcgggta atatcgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaatttc
    14521 tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac tcgctggcaa
    14581 aagcgttcca ccaggcgtaa gtcatgagtg atgaacaggc aggcagtgcc aaactgttgt
    14641 tgtagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag gttagaaacg
    14701 gcttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag gcagacgcgc
    14761 tggagctggc cgccgcttaa ctgcggtggg cgcttgtcga gcacgctgtc gtcgagatcg
    14821 accgcgtgca gcatttcgct ggcacgcgcc agttgttcgg attttttcag cgatagcagg
    14881 tggcgcatcg gttcccgcag gatctcgcgc acggttttgc gcggattcac ggcgctgatg
    14941 gagtcctgaa ataccatctg aatatcgcgg cggaacgctt tacgctgggc gcgattgagc
    15001 tttgccaacg attcgccacg ccagctaata tttccctgtg agggcgattc taagcctacc
    15061 agtagccgcg agagagtgct ttttccgcag ccgctgcgtc ccagcagggc gacggtttca
    15121 ccgcttttca gggtcaggga aacgttattc agcaccgcct ggtgctgatg ttttccgcta
    15181 aacccaccgt gggcatagtg atgggaaagg tcggagacgt taagtaaagt catgatgcca
    15241 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgttacc gcatgtttgg
    15301 gggcgttaaa cagcgtttcg acatcgccct gttcgacaat cttaccgtca gacattaccg
    15361 ctacgtcatc cgccagacgc gccaccacgc ccatatcatg ggtgaccagc agcatccccg
    15421 gcgcctgttt ttgcataatg ctttccagca gatcgagaat gcgtgcctgt gctaccacgt
    15481 cgaggtcggt ggttggttca tcggcgatga taaacggtga ttcgcaaagc accgccatcg
    15541 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc
    15601 gtgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
    15661 gtttccccag cgccaggcac gtctcccgcg cgtgggtgtg catggtgtgc agtggattaa
    15721 aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg gcgcagggag
    15781 aaaccggttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcct gcgggcagaa
    15841 ttcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg actaacgcca
    15901 gcacgcgccc gcgtttcagg gttaacgaca cgccgtgcac cagcggctgc gcggcctgta
    15961 gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg tcaccagatg
    16021 aggatccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
    16081 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact ggcgcgcgtc
    16141 gttaatcatc acgccccatt cggcggtcgg cgcggtcacg ccgaggccga ggaaggacat
    16201 cccggcaacg tgcagcatca tatggccgat atccagcgtt gccagcacca gcagcgaagg
    16261 gatcaccgca cctgccagat gatcaacaaa cactcgcaca tggcccgcgc cagaaagccg
    16321 tgatgccagc acaaactcgc gctggcgcag tgaaatcacc aggctgcgca ccatgcgtgc
    16381 ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc cagtgccgag
    16441 cacgccaacc ataaagaatg acagaatcga ggtcgggaag gtcataaaca tatcggcgac
    16501 gcgcatggta gcctgatcaa cgcgcccgcc aatcagcccg gcgctgccgc caataaccag
    16561 ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca cacgggtcgc
    16621 tgccatcagc cgcgagaaaa tatcgcgccc taagtgatcg gtgcccaacc agtgctgcgc
    16681 atccggcgaa agcaggcgcg acggcaaatc aatcgcctgg gggtcatacg gcagccacca
    16741 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgtaccga
    16801 ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatgcgtggg
    16861 tccagtgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccaccatc
    16921 atcagcgtaa agcactggat caccggatag tcacggttaa aaatcgccga caccgcatag
    16981 cgcccgacgc cgggccaggt aaagatgttt tcgataatca tcgtcccgcc aatcagttcg
    17041 ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag aatatgacga
    17101 cgctcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg acgctgaccg
    17161 gcgacgtcca gcatactggc gcgcagcagc cgtgcgttaa tcgccagcga cataaaggca
    17221 atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc gggtagccat
    17281 tgcagataca ccgaaaacgc catcaccagt aaaaatgcca gccagaagtt gggcatcgac
    17341 acgccgagaa acgcgatgaa acgtacggcg aaatccggca gacggtcacg atggcgcgcc
    17401 gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc acccgccagt
    17461 tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgctg cgtggcaaat
    17521 gagatgccga aatcaagatg cagcgccttc cacaaccagg tgccgtactg cacatacagc
    17581 ggctgatcca gtcccagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
    17641 ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag catcagaaaa
    17701 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacgtaa
    17761 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg
    17821 cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg taactgatag
    17881 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagt gcctgacgtt
    17941 gtgcttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggcttgtcgg
    18001 ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
    18061 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
    18121 gctgacgcgc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
    18181 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
    18241 acgaaagttc aatgtgcagc ggctgaccat ttttctcgcg gatgtctttg cccgcaggca
    18301 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
    18361 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
    18421 tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta agagcttcac
    18481 gtactgccag ctcattggtg ggggctttgg cggtattaag cgccagcatc acggtttcga
    18541 tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg aaggtatcga
    18601 gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca aacgccaccg
    18661 cgcgggtagt cgggtctggg atgacgttaa aagtgatctt cttaattgcc ggcttttcgc
    18721 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgcaaaa
    18781 cccacggtcc ggtgccaatc ggcgctttaa tcccgttcat ggtttcatgg tttttaaact
    18841 gcgagggggc gataaagcgg aaagggcgcg gcagggccag ttcttgcagg aaaggatagt
    18901 aggcgctttt cagggtgatt tgcagctctg ttttactgag tgctttaaca tcaacaatct
    18961 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg cggaagtttt
    19021 ctgccgccgc ctctgcatca aacggttcgc cgttagagaa tttcacgtca tcacgcaggg
    19081 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    19141 ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg gcgaacatct
    19201 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtggtga
    19261 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    19321 tgcggcggag tgtggagagc atggtaaccc caatggatta aaatagatgg cggaaataag
    19381 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    19441 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    19501 gggagtaggg gtgcagatcg ccaggcttaa attttctagc tgacaatggc tgactgacag
    19561 cgaggagtga taggtgctgt ctacgctgac gatttgccag gcgctgccac cacgctgttt
    19621 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    19681 cacggcgtcc atctcagcat gttccccgag gctgaacacg gcgttcgcca gccagcgcca
    19741 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cactcaacag
    19801 cagggcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt ccggcgcaaa
    19861 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    19921 caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa
    19981 acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    20041 atcagggcca acgtttaatg atgatggatg tattaatgcc gccaaaggca aagttgttac
    20101 tctgcaaaaa ctcacaatca accttgcggg cttcatgcat gatgtaatcc agcgcgccac
    20161 aattcgggtc tggtttattc aggtttagcg taggcgcaaa ccaaccttcg cgcatcattt
    20221 gcagactcat ccaggcttcg agcgcgccac aggcaccaag cgtatgacca aaataacttt
    20281 tcagcgagga gagcggcaca ttatcaccgt aaatggttgc tgtcgccaga ctttctgcca
    20341 tatcaccgcg atcggtcgcc gtaccatgcg ctgaaatata gccgatatct tgtgcgctta
    20401 acccggcgat tttcagcgat tgctccatac aatactgcat ggtttcgcgc tgcggctggg
    20461 taatatgtgc cgcgtcgcag ttagtcgcaa agccgacgat ttcaccgtaa atggtcgcgc
    20521 cgcgcgcttt ggcgtgctcc agttcttcga gaatgagcgt acctgcgcct tcaccaatca
    20581 ccagcccgtc gcggttttca tcgaacggtg aaggtgtggt tttcggcgca tcattgtgct
    20641 ggctggtggc gaaaagcgta tcaaataccg ctgcttctga aggacataac tcttcggcac
    20701 caccagcaac catcaccgtt tgataaccgt ggcgaatggc ttcccacgca taaccgattg
    20761 cctggctgcc ggaagtacag gcgctggagg ttgggatcac ccgcccgcgc aggccgaaaa
    20821 agaggccagt attaacggcg gtggtgtgcg gcatcatctg cacataggtg gtgccagtga
    20881 tgttattggt gtgcttttcc gtcagcatgg tggcgaactc gcttaccggg ccggtactgc
    20941 cggtcgatga accgtacgca atgcccgttt cgccgctggt taatatcgga tcgccaatca
    21001 accctgcctg ctctaacgcc agctcgctgg cacgggtcga catttgcgac acgcggccca
    21061 tcgcacggat acgcttgcgg gtgtagtgct ccggtagcgt gaaatcatca acaggcgcgc
    21121 caagcagggt gtgcagccca tcgtagacct gccactccgg cattttgcgc accgcatttt
    21181 catatgccag taagcgtgca gaaacgtcct gccagttttc accaaaggca gtcacgccgc
    21241 ccattcctgt aatcactacg cgacgtgtca taacatccct ccgttgatgg aaataacctg
    21301 gcgggtgacg taacccgcaa tatcagacat taaatagctg gcaagcccgg caacttcttc
    21361 cgcctggccc atgcgtttca tggggatcat cgacattgcc tctttcaggg cgctctcttc
    21421 catttcgatc atcccggtat caataagccc cggcgcgatg cagttgacgg tgattttgcg
    21481 cttcgcgagt tcgatagcca gcgcttttgt tgcgccgata attccggctt tggcagcgct
    21541 gtaattcacc tggccgcgat tgcccattac gccggaaacc gaggatagcg tgatgatgcg
    21601 cccaccctgg cgtgcgccga tcatcggcat aatgcacggc tgaatgacgt tgtagaaact
    21661 gtcgaggttg gtgtggatca ccgcatccca gtcgtcatcg cttaaagccg gaaaagcggc
    21721 atcgcgggcg atcccggcgt tactgaccac gccgtaccag gcaccgtgtt gcgcgatttc
    21781 gtgctccagc acttcccgac actgttcgcg gtttgcgacg tcgaaactga gcaaacgccc
    21841 gttaccccca ttggcgacaa tggcattcag cgtttcctga gcgcctgtgg cgtcacggtg
    21901 atagtgaacg ccaatgttaa agccgtctgc cgccagttgg caggcgatgg cgcgaccaat
    21961 gcctttgctg gcaccagtaa ccagaactga acgactcatc aggacgctcc ttgttgaaac
    22021 agggtggtaa gttcttccgc ggtcggctgg aaggtgttaa tgcggcccgt cgcctcgccg
    22081 tcgttgatgc tgcactcaaa actgccgaag cgatcgtctt gcatcagcag tttgacggta
    22141 atggtcagtg tctggcctgc gggcagaatg ccagtggtgc aaagcaattc acgcgcgccc
    22201 agtatcatcc ctaactcaat actgtttttg ccctgctggt ggcgatgcca gccagaccag
    22261 acgccgacgg tttgcgccat cagttcaagg gtaaaccagc cggggagatt gccgtccggg
    22321 tcgagaaacg gtgccagtac gccactgggc gaaaccgtca cgcggcacac cgcgctgtca
    22381 tcgctgacgc tcaccacgtc ttccagcagt aacatgggcg cgtcgtgggg cagataagcg
    22441 ccgggggata aatagtggct cacgaaaccc tcccgagcag aatgctggcg ttattgccgc
    22501 caaaggcgaa ggaattggac aggatcaccg gacgtgccag cggctgcggt ttttcgataa
    22561 tgccacacgg tggcagcgtg ggatcgcgcg gcgacaggct gaagtcctga gcgggcagcg
    22621 gtaaatcacg ctgtaaaatc aacatactaa ttgccgcctc ggtaatgccc gctgtgccca
    22681 gcgtgtgtcc ggtcaaatgt ttggtggagc tgcacggtac gcgttcgcca aacagggcgt
    22741 taaccaccat tgattcaatc tgatcgttga gttgtgtggc ggtgccgtgc aggttgatat
    22801 agccgacgtc atctggcgta agctgcgcat cggtcagcgc ctgattaata gcgcggatcg
    22861 ctccttcgcc ctgcggatgc ggggctgaaa tatggtaagc gtcgctcgat tcaccaacgc
    22921 ccagcaacgc gatgggctgc ggttcccggg tcagcagcat tagccccgcc ccttcaccaa
    22981 tggtgatacc tgcgcggtca cgaccaaacg gctgacataa ggttggcgac agcgattcga
    23041 ggctgtgaaa accgttaatc ggcatccggc tcagggtgtc tgcgccaccc acaatggcgg
    23101 catcgaccag ccccgcttcg ataagcctgc gcccgctgat tatcgctctg gcgctggaag
    23161 agcaggcggt tgaaagggta taggccgggc cgtcgagcgc cagccagtgg ctgagaaaac
    23221 gcgacggatc gccgagttcc tgttgcgcgt agtgccatgt ggtgctcggc tgcccgtggg
    23281 tgcgcaggtt aacgtgcgtg tcgccttcat gcaggccgga ggtgctggta ccaagcacaa
    23341 tcgcaatgcg ctcgcggcca tatttcgcaa tggcgtcatc aacctgcggc tggatctgcg
    23401 ccagcgccgc cagcaggatc tgattgttgc gcgagcggtg cgcagcgaat ttttccggga
    23461 taagcggaag ctcgccatcc acgccagcca ggaccgcctg cggatgcccc tgcaaccagc
    23521 ctgcgcgcgg gcgcatgccg ggagcaacac cgcgagtcag gttggttgcg atttcgtcaa
    23581 ggttatttcc cagggcgttg atcatgccga cggcggaaat ataaatcata tcagtcacct
    23641 aagtattgaa tggtgatgtg gtatttaaag acatgctgct caatgctgat tggcacgcgt
    23701 ttgcccttgc gattcagata ggtgatttcc gtgaccagtt tgccgctggc gttacgcagc
    23761 tcgcgtttgt caccgttgtc gcgaagtgtc catcctgcgg gaagttgcgg ttgccaggcg
    23821 ctaatcggcc agtggctgag catcacgtca gccagtacct gacttgcggg cggtaactgt
    23881 gggacgacga tggattgctc ggcgcgtagc ccttgcgcat cgtaggtcac cagaaacagg
    23941 cgaatgccga ccgacgacag cccggcaagg gtgattttct gatcgtcggc gttaagcatc
    24001 actaacagcg actgggtttt gccgttgaag ctgccggtca acagttgctg cgaattgact
    24061 gcgggtgaaa tccctggtgc cggcagcgtg atgcgcgtgc caggttgcag ccacgcctgc
    24121 gggcggcctt cctgttctgg ttgcgagtgg ctacagccgg tgaggataag cgtagcggtc
    24181 agggcgaacg ctcgccagaa tgtggatttg atcatttttt tgttctcttt ttatcgggca
    24241 tagccagcgg cgaaagcagg aaggcggtga aaataccgct caccagcaca atgccaaaac
    24301 tgctgatggc ctgggtggcg ctgaatacca gcatacccag cgtcagcaag gtggtgagca
    24361 ttgccagcgc gatcgccagt agcgaagtta acggtgtacc gcgcggatta ctgaaaaaca
    24421 gcgtgtagtt gataccgatg ccaagcacca gcaccagcgc cagcagcgaa aagagattca
    24481 ccgcctgccc gctcatcgcc agcaccgcca gaccacagcc cagcgaaagc accgaaggca
    24541 ccaggctgat aagccctttg cgccagccga gacgggccac tgcgccgcag gcaatcactg
    24601 ccagcgccac cagcaacaag ccggttaaga cgtagcggta aagtgcgaac aattcatcaa
    24661 aggtgctttt gcgatcaacc caggcaatgc cgcaagggta atatgtggcg atttcctgca
    24721 tcaacgcgct acttttaacc ccttcaaccg gcaccagtac gccgctttcg ccgttttcca
    24781 gcgtcagcca cagcagacgc cagccttcac tggcggggct tgccagccat tcatcaacgt
    24841 tcactggcat ggcgttgaga tccgggttca ctgccgtcag cccggcattt tgcagcgctt
    24901 ttgttactgt cggggccgcc gttttcagca gttgtaaatc ttcctcctgc cgcgcaaggg
    24961 agttcagcgg aatggtgcgg tagttgctga taagcccctc ttttttcgca tactcaagtg
    25021 aggcggtata tttctccagt cgccgcaatg tttgctgtgg cgaatcgccg taaaccacaa
    25081 accatttttg atcgacgctc tgcccggtca gggcggtaat ggctttttcc tgcgccagaa
    25141 tatgctgcgg tagcgcctgt aactgcgaga tatcgtcatc gacgcgtagc attgacatcc
    25201 ccgccagcga aaacagcgcc agcgcgacgg gcagacccag cgacagtttt ttattacgcc
    25261 gccacgctgc cagccagcgt agcatcagcg ccatcgccgg aaccggacgc accggcaggc
    25321 cacggcacag ccacggatgc cagaacagga cggtcagaca ggaggcgctc aacccgacgg
    25381 cggcaaaaat cgccatctgg cgaatgccgg ggaagggggc gagcatcata atcagatacg
    25441 ccgccacggt ggtgagcagc gccagtagca aggcattgcg tactttcgcc aggctttgcc
    25501 acggcgaaac gtcgttgccg tgaaccatcc gctcggtgag ataatagagc gtgtagtcag
    25561 cggaaatgcc gataacgctc atgctcatca ccagcgtcat caggtgtaat tcaccgaaaa
    25621 tcaataaagt ggcgaccgtt cccgccagcg cgccgatgcc gatggaaatc acgcaaagca
    25681 gcaacgggcg taaagagcgg aacaccgcca caatcagcaa tatcaccccc agcagcgtag
    25741 ccacgcccag ggtggagata tcctgcttcg cctgttggct ggcgtaatcg ctgtaaaaca
    25801 ccgtgccgcg cgagagcaac tgtgcctgcg ggtaacgcgt tttcagatcc ttttccagcg
    25861 tattcagggt cgtgatcagc tggtgggttt gctgcatatc aaacgacgat cccgccagtt
    25921 cgccgtgcag cagataccag tagttgccct ggggatcctg cgtcaccagc caaccgtcca
    25981 tcagccgcaa acgctggccg tttttcgcca ttgccagctg cgagccgcgc attaacatca
    26041 gcggatcgtt ttgcagctcc ttgccgctta cgccggagaa tgcggaataa agctgggaga
    26101 ggatccactg tgcctgcgct tcgccgccgt tttgcaggcg ggcgcgggtg ttggggtcaa
    26161 tcaggccgtt gcgatgctgc cagaaaaacg ctccccacgc ctgctggctg gcggcatcca
    26221 ttggcccttt aacgtcgccg agcgcagcgg atttttgcag cagcgtcagc cactcctgag
    26281 cgaccaaagg attagcctct ttaccggggc tgaccagcca caccagttgg cggtcaagac
    26341 gctgcataaa gccgtcattc agcgccggag gaatatcgcc catcgtctgt ttgggtaaca
    26401 tagccagcac gctactgttc agccgtgatt gcggcagcag gatcagcaac gccaccgcca
    26461 tgaccaggca gactagcccc cataacagcg cggggcgttt actgggcggc aaaacgttgg
    26521 cgttcgtcat cggtcagttg cgctggcgtc agttgatgtt gggtaagagc aatatcggtg
    26581 cgatcgccct gtttatcatt aagttgaatg ctctccagat aggttttccc ggcgagatcg
    26641 atggtgttga aaattttatc cagcggcgtg gtggtcggcg tcaggcgcag cgtccagcgg
    26701 ccttcgcctt tgtcagcaaa ttcgacgcgg aagttttgtt ccagcacttt gcgatcggcc
    26761 tggaacaacg cgcgcagcag gtggttaaac tggaacatct gcgggttgtt ttctgccgtg
    26821 atgatttgcg gcggctgacc gttgatcacc tgcaccatgc gtttatcgtc cagtaatagt
    26881 tgcatgggga acggtgaggt ttgatcccac aacagcccct ggtcgcgggc gatcaacatc
    26941 tggccctgag atcgcagcgg ctgcggcaga tctttaatcg tccgggtttg atcgaaatgg
    27001 gcgcggatca ccggttgttc ggtaaagcgt tgctgaagat cgtccagggt cagggcgctc
    27061 acaaacgggc taatcagcag cgccagcagc ggtaaaaatt tcatggtttg actcccatgc
    27121 gttcaaacag aatatcgggg ctgacaaagc acagttcgcg actttgctcg tcgaccgcga
    27181 cctgaatggt gtatccggtg gtggcgcgtt tgcccgtttc ggcatcgaaa atctgatagc
    27241 caatccgcag acggttttcg aactcttcga tatgcgcacg cacgcggatg cgttgctcaa
    27301 aggtgagggc gtggcgatat ttcacccgcg catcgaccac tggccacaaa taaccggact
    27361 ctttcatttg ccgatagccg taattgaact ggttgagcag cgcctcgcga gccacttcaa
    27421 agtagcgaaa atagttaccg tgccaggcca cgcccatcat atcgacgtcg tgaaagggaa
    27481 tggtcagctc gacttctgcg gtaaatcggg gatcgttcag caccctttac tccttgtcct
    27541 gaatttccgg cagttgccag aaatcgaaaa aattaaacca gtcgagaggc gactggagcg
    27601 cgtaatgttc cagacgcgcg gcgtaatgat cgatagcgtt ttgcagcgcc tgttggcgtt
    27661 cgccgcgcgg cagctgcagt gggtcggcaa agctttcgca gtgaatatgc agcttgccgt
    27721 gctggcgcag ggcgaaaatc agattcaccg ggcagcgcaa aatagaggcg agaataaacg
    27781 gcccctgtgg gaaaggcgca ggctggccca taaacgaact ccagcagacg cgccagtcgc
    27841 cgccgcgttg cgggttgacg gcgatgcggt caccgacaat cgccacccat tcgccgttat
    27901 ccagcttctc tttcagcagg atggcggttt ctgggccgat atctgttacc ggcatcaggt
    27961 taatgcctgc ctgaggagcc atctcctgca ttatctgttt aaagcgttgg gcgttttcgc
    28021 taaacaccag cgcgttaatg gttttgtagc cctgaatttt tgccagcgcc cggcacactt
    28081 ccacatcgcc aagatgcgag gccagcagca atttgccgcg cggatcgcgg acgtcaagcg
    28141 ctgcttctgc gcctggcgca aacagcacat cacgccctgg ttgtagctcg ccgcgccagc
    28201 tggcgatttt gtcgagcatg gcattaccga aacgtagaaa gtgctgatag ctgttgagtt
    28261 ttgccgcctg tggttgatgc tgtcgtacac gggcgagcca gtcttgcgac gctttgcgcg
    28321 cacgagaagc agtgagccag tagacgccca ccaccgggta aagcagcgcg ctaaacgccg
    28381 ttcttcccag cagacgccag accagcagca tcaggcgcat tccccataat cctttcactt
    28441 cactctgccg cgcccagtgg caggaagagc ggcgcatcag cagtgaagga atacgcggca
    28501 acatgccgaa aaacagacgc gtgtgcatga gcgagatgcg gacgttatct ttcagggcat
    28561 caaaatgcga aagcccgtcc agtggatagg tgacgcgggt cggcacgaaa tagctggtat
    28621 ttccctgcca gtagaggcgc accatcactt cggtgtcgaa atccatccgc ttgccgatgg
    28681 tggcgtgttt tgccagttgc agcgttggcg caaccggata aacgcgaaaa ccgcacatgc
    28741 tgtctttcag ttgcagggag agcgtttcga tccataccca gacgtgggtg acccagcgcc
    28801 cgtaaagccg tgagcggggg atggagtcat cgtaaattgg ctggccggag atcagtgccg
    28861 caggttgttg ttcagcgaga gccagcagtt tagggatatc ttcaatcgcg tgctgaccgt
    28921 cagcatccac ctgcaccgca tggctgaacc ctgcgtctgc cgctgcctgt aagccacgca
    28981 tcaccgccgc gcctttgcct gcgttttctg ccaggcgaat taaggtcacg ccaggctgtt
    29041 cggcaagcag actgtccagt tgctgttgtg tggtggcatc gctgccgtca tccaccacaa
    29101 tacagggcag attaaatggc ttaagacgcg ccagcacgcc cggcatcatc gcgccgtggt
    29161 tgtagcaggg tatcaacacg cagggagaaa agtttaccga cataggcgga tcttcccgct
    29221 actggcggtg tggcgggcgt caccgtcgtg acgctgatag ctaaagctca gaatctggcg
    29281 ttcttcctgc cagttaagcg cgagcgtgac gcggttttcc ggcagcaacg gagactggaa
    29341 ttttatgttc tgaatggagt gaaaacgcca gccaggcgcg agcagattga gcgcgtagct
    29401 catcgcccag tcgatttgcg caactcctgg gagtaatggt tgcacggcaa aatgaccgcg
    29461 aaaccagaac agatccgccc tgagatgcag aacaatttca aggtgattcg tttgtgcctg
    29521 atggcgctcg atttcatggg gcttcatgaa ataactcctg taattgcgca tagacacgct
    29581 tgttcatact gtttactggc atttcatcaa taacgcgcca gtaacgcgga atagcaactg
    29641 gctccagcgt cgggcgcagt aaacgccgcc aggtcatttc ctggctatgc ccgccgccgt
    29701 tctgccattg cagatgagct tcgtcatcca gcaccagcaa aacgcctatg ctctgacgcc
    29761 cgccgcgcgt aacgggaact gccgccgcct cgcgaatgcc gtccagcgcc agtagacgtt
    29821 gttctacttc ctggagtgaa atacgttttt cttcaatctt aacgatgcgc ccacgacgcc
    29881 ccatcagatg gaactgacca tcttcgctaa actgtaagat atcgtcgagt aacatgccgt
    29941 tatcttccgc tatcagcggt gaaaagaggc gaaaggcatc gccttcggcc tggaactgca
    30001 tacctgggag gggtagccag cggcgttgct cttgttcgcg gtagcgccag gcgatgacgc
    30061 cagtttccgt gctgccgtag atttcatcag gccagacgcg cagccagctc gcggtatgtt
    30121 gtacatcctg ccacggcagc tcaccgccag cagagagaag cacctgaacg ggtggcggag
    30181 aaagctgggt atccaggcgc ttcaaaaatg ccgggctgct gatgaagata tagcgatgct
    30241 gatgactcaa cgcggcaaac tgttcgacat accagagcat ggcggcgtgc agcggtaatc
    30301 ccaacgccat gggcaggaaa acgcgaaaag tcaggccgta caaatgctgc ggcaatacgg
    30361 aaccgacgac acgacaaccc gccagacgtg cgcctaatcg ttcagccagc agttctgctt
    30421 cacgatccag caaatgaaca ggtttaatcg cccgtttcgg ctgtcccgtg gaaccggagg
    30481 taaacagctc gataaaagcc tctggcgcta tagcggcaaa ggtgaaggat tgtgtcgcga
    30541 tttgtggaga acttgctacc aacagcaatg aaccttgcca gttaagttcg ctgtcgctaa
    30601 gcacaccatc aaaaagttca cgttgctcgt taagctggat aacacggtta tgccccggaa
    30661 gcaccggcgt ttttccggcg tgtagcgtgg cgagcagggc aacgataaac agatagctgt
    30721 tctcaaagca cagcgcccag cgttcacccg gctgttgctg caagcggcag ataagttgcg
    30781 cgacatcgtg acgcaaatcg cccagcgtcc aggtactttc gttcagccac gcaatgggtg
    30841 tgtcatcagg gcgtggcgca gtcagccagc gtgcaagcgg taaggtctgc ttcatgcgcg
    30901 atctcttttc atcacctgac ggcgcagcag ccattcacca gccattaacg tccccatcaa
    30961 caaataagca atcatgccgt tccaggcggt ccataacgac atatcggcat aaagcgcggt
    31021 aaagagggca attccaccgt taatgatgaa aaagccgcac cagatttgcg tgacgtggcg
    31081 ggtatagcgc acgccctttt ccgggaggtc tggctcctgc aaccgcgcca gccgctcaac
    31141 tatcggcatt gccgaccaca gcgaaccgcc aaaaaccgcc agcatgaccg cgttaaccac
    31201 taccgggtaa aacagcagta gctggtgcgt tttcaacata aagctggcga cacataatgc
    31261 gatgcccacg acagcgacga gttgcgtgac ggcttgtaaa ggccccgcct ggcgacgggt
    31321 ctggcgcaga cgcaacaaaa gcagcaacgc cattagcggt aacagccagt gcaggccgtt
    31381 atgcgctaag ccaaaccaaa tcacaaacgg ccaggcgagc agcaataatc cagtcagcag
    31441 cttaatcatc ggtagcgagc gaatgcccga catcagatca ggcttcttgt agcaggcgtt
    31501 ctacagcctc gacgacatcc tggactgtac gcactgcttt aaactcttcc ggcttgattt
    31561 tcttaccggt tttcttttgc aggtgcacaa tcatgtcaac ggcgtcgatg ctgtccagtt
    31621 ccagatcttc gtacaggcgc gtttcaggtt tgatgtcctg cggatcgatt tcaaacagct
    31681 taaccagcag ggctgagact tcctgataga cggtttgttg ttctgtcatg atggctttcc
    31741 tcaggcacgt tgcgcagcaa tgaaagaggc cagagtggcg acggagaaaa agtgctgacg
    31801 catctcttcg ctttccgcag agagcacgat gccgtactca ttttttactg ccagtcccag
    31861 ttccagggca tcgatagagt ccagccctaa accgtcgcca aacagcggcg cgtcggtatc
    31921 gatatcatca ggcgtcaact cgtccagatt gagggtgctg atgatgaggt ttttaatttc
    31981 cagataaagc gcttgcatta tttattcctg agagaggtaa acaaccttgt tgtaattgaa
    32041 gcaaaagatg ccggtttaac tgccttgctg ccagcgccgg ttcttgtgaa gttgcatcgt
    32101 aaaatttatt gatttcaacc cgaccatgga ctgcaaccgt aaaaaacggc cggtcaggtg
    32161 gaacgtcata ccatttactt tgtttatcca gcagatgctc gctacagccg attgtcacga
    32221 tgcgtaaatc gctggcgcag cgcacggcaa tattcgccgc cccgcgctgc agcgtcattt
    32281 tttcaccggg cttcgttcgt gtgccttcgg ggaaaatcag gatagtgtca ccctgtgcca
    32341 gtcgctgttg gcagcggggt aacagagttt cagcttcgct gttgattaaa taatctgtcg
    32401 cccgcacgac gccgccaaga aaaggatttt ttaacagcgc gcttttcacc aggcagtcag
    32461 tttctggcat gactgacgcc agcagcacgt aatcgatcag cgtgggatga ttggctacga
    32521 cgaggcaacc gcgttcctga tgtaagattt cagcgccatc aatacgataa tccagtacgc
    32581 caagcccttt cgcgacagtt aaaaacaggc gaaaactggc ggcaatgctg cgacgcgcca
    32641 aacgacggcg gcgggaagta tcccacacca aaactaacaa tatgttaaac cacacgacag
    32701 aaagcagcag gccgccgaga ccaaacaggg caaaacataa cccggtcatg accagtcgcc
    32761 aggtccactc cagacgtgac atcagcttac tcatctgtgg ctccagcgcc attgcacgcg
    32821 ctcgccaggc agtgaaaatg tgtctacatt ttgtaaatag tgctgtaaaa acaacatgct
    32881 ttgcggcagt gttgtttcat tgcccgcaat ggctgactgc gtttcacatt gccagtcatc
    32941 gccagcttca atcaccagcg cgacggcgta cggccaggtc ggcatttctg atggcagttg
    33001 tgggtgataa aactcgggca ggaaaccgtc aaaatcgacc atcagcacgc gctggtatcc
    33061 cgcttgtagc aggctaagca cttcacacaa accttgctgg aaactatcgc gaccagccga
    33121 aagcgatgac gaaacgctcg gctgtttggc cgcaatggtc aggttaccca cggaagagtt
    33181 gtgtacggag agcgcgaagt ccgtcggaga aagcgcctgt tcggttgcca gtgcatgaac
    33241 aatgcggtaa ttgcgctcca gttcgccatg acgactggta tacaaaacgg cgtctggttg
    33301 atggcggcgt agcattgcca gaccgcattc aacagccagt ttgctcccgg agctgaggcg
    33361 gcgggcggtc atcattggca gttcacttaa ttttgccaac ggtgctgtcg ggtcaattgc
    33421 ctgaggctga cgtgaccact cctgccattg cagcgtttca ctaagtccag gcgctcttgc
    33481 ctgccagtcg agaatgttta atgcaaattt catcaacgcg cgcccgcggt aaaccaaaat
    33541 tattaagtga cgattatttc ttttggcata tcaaaagtgt atgcccaacg cctaaattat
    33601 cgtggcgctc tgctacctgg aatcctgctt tattcagata gtcgtaaaat ttctctgcac
    33661 tatagaaacg gctgttaccg tttgccatgc aggtaaaata aagtgaggac gcgttcaggc
    33721 taaacgatgc tgcttcaaac cgctgcgcat cccagaacaa ctccatgatg caaacttttg
    33781 cgccaggctt cattacgctg gcaattttgc tgagtatgct aattatttgt tcgggtgaaa
    33841 aacagtctaa aaattggctc atccaccaga tatcagcctc gcccggtaat ggggcatcgc
    33901 ttagcatatc caccgcatgg aaatctatgc gatcagaaaa acctgcattc gcgatatttt
    33961 ctttcgctag tacgatttgt tgtggcagat ctaataacgt aacggcgatg ttttcattgt
    34021 atttgcagca acgcaaagcc cacttacccg tattgccgcc tacatcgtac aatgttgtgg
    34081 ggttgttggc gaatacataa ggcagtgcgg cgttaaatgc gccatctgaa tagtaatgat
    34141 caaacgcaaa ccagctttca cgagcggcgt ctggtaattg cgaaagtgcc ggatatatcg
    34201 tcgaccagtc gccaaatact tttaacccgg aaggttttcc ttcattgagt gaatcggcga
    34261 ggaagaagag cccctggtaa cagacatcct gggtgaaatc catattcacg cgagtcatag
    34321 tgtcatgcag gaggtaatga ccgattttcg ccaggtaata aattccctct ttacaggtaa
    34381 taattcgccc gcttaagccc atatctaaca gaacgctggt agcatattcg ttgatatggc
    34441 tatgttcggt gatatcagcc agcgttgcgc catgtttgcc ttgccgatcc agatatgaaa
    34501 gtacacccgc attacgtagg cagagtgctg tttgaaaaag cataggggca aaggcaatgc
    34561 gttgagcttc ggtaatcgca tccagtgcgc taagagaatc ctgttcgtac atgttgggca
    34621 caacctgtta agagggaatt aaattattat cggaccgcaa cggcccgact ttaggaagaa
    34681 ttataaatct gcgcccgctg acaaattcag ttgaatatct ttgtcgagat tgcggaccca
    34741 gcgattgtag tttttatgaa tttgtccgcc tgacgcttgt aggttctggc tattttcata
    34801 attgatatcg tagctggatg ccgtgtagtt aatgcgtatt tccgctgaat gatcgcgtga
    34861 ctgttgacgt gctttaatta tgccttgtcc ggcttcggac ataatccatt tgcgttcaac
    34921 gccagctttc agaatcgctt ttttcacttg ttcctgggta tgaccagcgc tgacggttga
    34981 gtgaacctga tcaattggcg cagtacgcgc gcaacctgct aaagcaccca caacagcaat
    35041 agccgcaaac catttcatgt aatgtctcat tagaacccca ttaaagtatt gtttttacga
    35101 gtaaagcaag ttttaactgt aattgctggc atcactattc tttatttaga gaaaaaatta
    35161 cttgtgagat aagtcagtaa gtgcacatca gagtaaaaat ctctgaaatg actaaattac
    35221 tgccagcagg gaccaactat aagtgagtga gaaaaatgag gcaataaaaa taacacaatg
    35281 ttatcagtgc attctttgag aaggatttaa tcgctattcg gcgtttatat atatttgact
    35341 taaggaatgg tttttttaaa ttaattttct gaataatgtt tcatcatttc ctcaaatcaa
    35401 atggccactg ttagataaac ggtggccatt gcctgctcag gaggtgctat taacccggta
    35461 accgactttt cggtctgccc ggccccagta aaatcgccag tttgctgccg cctttcgtcg
    35521 tttccatcca gattttacac acgctggtta atggcaccga aagtagcatc cctaccgggc
    35581 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    35641 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    35701 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    35761 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt aagcaaaaac gccagtactg
    35821 cccacatcag cgcaaactgt acccccatca gctccagccc cagccagacg atgacacccg
    35881 tccataaact gagtagcgtc tttaacgcca gataatgcga aacgccttta agtgcgcggt
    35941 gtaatcccgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    36001 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    36061 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    36121 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    36181 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    36241 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    36301 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtgacg agcgggttga
    36361 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    36421 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    36481 cgggttgagg ggtttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    36541 tcacgacggc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    36601 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    36661 aacggattgc gcctgaattt gcgcattctc tccattgtca tgtttaactt cgccagctac
    36721 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    36781 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    36841 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    36901 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    36961 gtcatcagcc tgttattact ttgcctggga cgcgtcatcc ttgggattgg gcaaagtttt
    37021 gccggaacgg gatcgaccct gtggggtgtt ggcgtggttg gctcgctgca tatcgggcgg
    37081 gtgatttcgt ggaacggcat tgtcacttac ggggcgatgg cgatgggtgc gccgttaggc
    37141 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taattattat gggcgtggcg
    37201 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg
    37261 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcgct ggcactggct
    37321 tccgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgt taaaggttgg
    37381 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    37441 ttccctaacg gcattaaccg tatcggcggc ttaaacgtag cgatgatttg ctttagcgtt
    37501 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc
    37561 gtcttactgg cgggggccgg gttttcgctg gtgttcccgg cattgggtgt agtggcggta
    37621 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cctacaccgt atttatggat
    37681 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagttgggc gggcgtaccg
    37741 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    37801 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    37861 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    37921 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    37981 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    38041 ctcaatggct ttattggtga ccacttgcag ctgtggatcg cggctacgtt gctgatcttc
    38101 cagacgcttc gccagcaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    38161 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    38221 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    38281 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    38341 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    38401 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    38461 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    38521 cagtcttttc aacagcatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    38581 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    38641 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    38701 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    38761 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    38821 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccatgaac ccatatagaa
    38881 tagcgacgag atgacgtagg agatcagcaa tgccgggatc atcaccgcaa agatgatacg
    38941 acgggccagc ggtgcgccaa aaatacgtac ggtcaggtcg gtagcgagaa agataaacgg
    39001 aaagctaaac gcgccccagg tggtatggaa accaaaaatg gagacgggaa gctgaaccag
    39061 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    39121 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    39181 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    39241 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    39301 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    39361 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    39421 ctgcccggaa ccagtcatga tggtgcgcaa aacagtgcgc aatatgcagc caggtgaaac
    39481 attgctgatt attgctgacg atccggcgac gacccgcgat atccctgggt tttgtacctt
    39541 tatggaacac gaactggttg ccaaagagac ggatggactg ccttatcgtt atttgattcg
    39601 taaaggcggt tgacgggggc agattggctt cgatgccgcc ttttcccctc accctaaccc
    39661 tctccccaga ggagcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    39721 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    39781 cactgccagc catagtccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    39841 ccccagcgca atagtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    39901 catttgcacc agaccgcgca ggtggttatg ggttaatgct gcgtcggcgg tttccagcgc
    39961 cacgtctgtg ccgctgccca ttgcaatccc gatggcggca gctttcatcg ctggcgcgtc
    40021 gttaataccg tcaccgacca tcgccagcgg cgcatgttga ttcagctcgg tcaccgcttt
    40081 gactttatct tccggcaaca ggcccgcttt aaactccagc cccagctccc cggcaattgc
    40141 cgccgctgcg cgtggattat cgccggtgag gatcacccct ttgacgccca gcgcgttcag
    40201 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    40261 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    40321 caaaccagca aatgcatcag cgggatgttt cccggcagcg catatcaaca cgcgctcacc
    40381 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggcgggaat
    40441 ggcgagtgca gcaacctgtg cttcgcgcac gatggcctgc gccagtggat gcgtcgcgcc
    40501 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    40561 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    40621 ggtaacacga cccagctgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    40681 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    40741 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg tggcaaacag
    40801 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tgtaaatacg
    40861 gctgaaacgg tcgataaacc gctcaatggg tgcgcgacgc tcttcggctt cttcgatcag
    40921 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    40981 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gttgcgcgct ccaccggaat
    41041 agattcgccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    41101 ggcaggcaaa cgtccacctg cggcgacttc aatcacatcg ccagggcgca ggctgttaat
    41161 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    41221 taacgcgctg accccttgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    41281 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    41341 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    41401 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    41461 gaacggatga ttaaattgct ccaaacccca gctgattgcc atcatcacga taagcgtaat
    41521 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    41581 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacggatgt cattgtcggc
    41641 atcaaccacc agtttttcgg tggcgaacaa cacctgtacc tgattcacgc ctgcaagctg
    41701 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccgc tgactttcca
    41761 gctatagcgg gtgccggaga cgttttcaga gagagttggc gtgctggaac atgcgccgtc
    41821 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    41881 ggctttcttg ccgtgattgt caggagtcga catggcatcc tccggttaag ttttttctca
    41941 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagcg
    42001 agcggacgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttgtccgcac
    42061 ggaagcgtcg gcgatagtga ctacccagcc agacaaagtt actgataaac agcaacgaag
    42121 cgccgacaaa cgcagagagt gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    42181 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    42241 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    42301 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    42361 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    42421 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    42481 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    42541 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    42601 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    42661 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    42721 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    42781 gttatcattg gcttaatcat cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    42841 aaagctgata atgatatggc accactccag caaaagctgg tgttggtgag caacaagcgg
    42901 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    42961 atgcgctatg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    43021 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    43081 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    43141 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gcaccttttc ggcggtggtc
    43201 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagttgcat accatgcatc
    43261 agcacggtaa aaatcagcgc cacgttaacg aaaatattca gcgggcgcgg gaagggatgc
    43321 accaggttga ggattaaaaa tccccaaaca cagagcatta acaaacgacc aatattaatc
    43381 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    43441 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    43501 ttcgacataa atcagggttt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    43561 ctcttctaac aaaccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    43621 tttttgcgcc aggaatgaca ttgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    43681 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    43741 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    43801 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    43861 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    43921 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    43981 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    44041 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    44101 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    44161 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaacgg
    44221 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttctgaacaa gccgtcgaag
    44281 agcagccgca ggcgcatact gaggccgagg cggaaacttt tgctgccgac gttgtggaag
    44341 tcactgaaca ggttgctgaa aatgaaaaag cgcagcctga agcggaagtc gttgcacaac
    44401 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    44461 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    44521 ccgtagagat tgtcgaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    44581 agctggaagc acaggcgctg gctgccgaag cagcagaaga ggcggtgatg gtggttcctc
    44641 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccgaccaaag
    44701 aaggtttttt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    44761 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    44821 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    44881 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    44941 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    45001 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    45061 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    45121 gtgcagcagc ggttgaacag cttcaggtct gggggcagcg caacaatatt ccggtgattg
    45181 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcggctaaag
    45241 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    45301 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    45361 atgaagttat gctgactatc gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    45421 tgttccatga agccgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    45481 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    45541 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    45601 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    45661 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    45721 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    45781 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    45841 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    45901 tactgtctac gataacgtgg cgatcccgct gattatcgcc ggtgccagcg gtgacgatat
    45961 tcgccgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    46021 ccctattcag ctttctggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    46081 caagcccgcg gtactgctgg cggacgaacc gactggtaac ctggacgacg cgttgtcgga
    46141 agggattcta cgtctgtttg aagagtttaa ccgcgtaggg gtaaccgtat tgatggcaac
    46201 gcacgacatc aacctgattt cacggcgttc ctatcgcatg ctcaccctga gcgatggtca
    46261 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    46321 gcgggcgtct tgatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    46381 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    46441 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    46501 catttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    46561 tgtacaaaaa cgttaaccag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    46621 atctgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    46681 agcaaggcgt agagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    46741 actggtctgg ttttggtggc gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    46801 cggtggtgat cccgaagctc gactttcagg ggacggagtc tctgaatacg ctgcgtgatc
    46861 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    46921 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    46981 cggcagtgtt cctcgtcatc ggtaacagtg tacgcctgag tatctttgct cgtcgtgact
    47041 ccattaacgt gcagaaactg attggtgcaa cggatggttt catcctgcgc ccgttcctgt
    47101 acggtggcgc attgctgggg ttttctggcg cattgttgtc attaatttta tcagaaattc
    47161 tggtactgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    47221 atatcaatgg tttatcgttc gatgaatgcc tgctgttgct gctggtatgc tcgatgattg
    47281 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    47341 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gaccccgttg
    47401 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcctgc
    47461 attgaacttg tggataaaat cacggtctga taaaacagtg aatgataacc tcgttgctct
    47521 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    47581 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    47641 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    47701 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    47761 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    47821 caggaaggta acatcggcct gatgaaagca gtgcgccgtt ttaacccgga agtgggtgtg
    47881 cgcctggtct ccttcgccgt ccactggatc aaagcagaga tccacgaata cgttctgcgt
    47941 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    48001 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    48061 gaactgggcg taaccagcaa agacgtacgt gagatggaat cacgtatggc ggcacaggac
    48121 atgacctttg acctgtcttc cgacgacgat tccgacagcc aaccgatggc accggtgctc
    48181 tatctgcagg ataaatcatc taactttgcc gacggcattg aagatgataa ctgggaagag
    48241 caggcagcaa accgtctgac cgacgcgatg cagggtctgg acgaacgcag ccaggacatc
    48301 atccgtgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    48361 tacggtgttt ccgctgagcg tgtgcgccag ctggaaaaga acgcgatgaa aaaactgcgc
    48421 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    48481 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    48541 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    48601 tagcagagta tgctgctaaa gcacgggtag tcatgcataa aacgaaataa agtgctgaac
    48661 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    48721 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    48781 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccag
    48841 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    48901 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    48961 gcgaacaaag tcgttaacga cggcattaaa tacgtgattg gtcacctctg ttcttcatca
    49021 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    49081 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    49141 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    49201 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    49261 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    49321 ttctcaacgc tggtggcgcg tctgaaaaaa gagaatatcg acttcgttta ctacggcggt
    49381 tatcacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggtt gaaaactcag
    49441 tttatggggc cggaaggcgt ggctaacgtc tcgctgtcta acattgcggg cgaatcagcg
    49501 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcgaa caaacccatt
    49561 gttgatgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    49621 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    49681 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacctg ggatgagaaa
    49741 ggcgatctga aaggctttga gttcggtgtg tttgactggc acgccaacgg cacggcaacc
    49801 gatgcgaagt aacagataga taaatgaaaa tgcccgatgc gctgaactgc catcgggttt
    49861 ttttgtgttt gtcgaaatgg ttgtagaaag attaaacaat ccaggatatg cttaatgtac
    49921 ggtgacatac cgtacatatt atacggagca ggagagttca catgtctgct gttaaaaagc
    49981 agcgtatcga tctgcgttta actgacgatg acaaaagtat gattgaggaa gcggcagcga
    50041 tatctaatca gtccgtcagc caatttatgt tgaacagcgc ttcgcagcgg gctgcggaag
    50101 tgattgaaca gcatcggcgg gtgatcctca atgaggaatc ctggacgcgg gtgatggatg
    50161 cgctgagtaa tccaccgtca ccaggtgaaa agctaaaacg tgcggcaaaa cgtcttcagg
    50221 gaatgtaata atttatggat gatctgacga tagagattct gaccgatgat gcagattatg
    50281 atctacagcg attcgactgc ggcgaggaag cgttaaatct ctttctgacg acacatctcg
    50341 ttcgtcaaca tcgcaacaaa attctgcgag cgtatatcct ttgtcgcaac actccagaac
    50401 gtcaggtgct gggatattac acattatgcg gcagttgttt tgaacgagcc gcattgccct
    50461 cgaaatcgaa acagaaaaaa attccctaca aaaatattcc cagcgttact cttgggcgtc
    50521 tggcaattga tcgttcatta caggggcagg gatggggagc aacactggtt gctcatgcca
    50581 tgaaagtcgt ctggtcagcc tctttagcgg taggtattca cggtcttttt gtcgaggcgc
    50641 tgaatgaaaa agcccatacg ttttataaat cgctgggctt tatcccttta gtcggggaaa
    50701 acgaaaatgc gttatttttc ccaaccaaat ccattgaact gctttttaca cagagcgatt
    50761 aacgcttctc ccagccgccc tgctgtgccg taaatcccag tgcctgcata aacgccgtca
    50821 tcacaccgcg atcttccacg cctgcatccg ccatccacca gcatgaaacg ccaggattgt
    50881 tacgcaaaac ctcttccagc agatattgcc ccacaccgcg acgtcgagtg acttcccgca
    50941 cgcgcaggga atccagtgcc ccctcggtgc cgcttaaggt tacccgcacg gcagcaagca
    51001 ggcgctcgtt aaaacgcgcg gcgtagatgc ggtggttatc gtcaacctgt aaagaggaag
    51061 gggaatactc cggccagatc ttttgcaggt caatccggtc ctggtcgcta aatttttcta
    51121 atcgaatgat ggtcagcttc atgggtaacc cgtgtaaatc acaaaagtgg aaccagtgta
    51181 gcgaaataat ttaatcggag gctttctctt ttttatttct tttggcaggt gattaatttc
    51241 ttaacagcaa taattacaaa gttaaaacat tagagaatga aaaataccca gcata
//
LOCUS       DAZKFZ010000010        49461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000010 DAZKFZ010000000
VERSION     DAZKFZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1079
                     /locus_tag="ACHOQW_000571"
     CDS             <1..1079
                     /locus_tag="ACHOQW_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9571702.1"
                     /translation="GCDNTQSNNSSPSETEVGVVTVKSQPVSVVSELTGRTSAALSAE
                     VRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARAALQQAQALVKADCQK
                     AQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETARINLDWTTVTAPISGR
                     IGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRLRKQSLATNSDTMSVS
                     LILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLLPGMFVRARVDEGVME
                     DAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDKWLVLNGLHSGDRLIV
                     EGSAKVTSGQTVKAVEVQANGGNA"
     gene            1079..4187
                     /locus_tag="ACHOQW_000572"
                     /pseudo
     CDS             1079..4187
                     /locus_tag="ACHOQW_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            4191..5564
                     /locus_tag="ACHOQW_000573"
     CDS             4191..5564
                     /locus_tag="ACHOQW_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFP9571703.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            5573..6736
                     /locus_tag="ACHOQW_000574"
     CDS             5573..6736
                     /locus_tag="ACHOQW_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFP9571704.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(6788..7594)
                     /gene="sapF"
                     /locus_tag="ACHOQW_000575"
     CDS             complement(6788..7594)
                     /gene="sapF"
                     /locus_tag="ACHOQW_000575"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP9571705.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(7596..8588)
                     /gene="sapD"
                     /locus_tag="ACHOQW_000576"
     CDS             complement(7596..8588)
                     /gene="sapD"
                     /locus_tag="ACHOQW_000576"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP9571706.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(8588..9478)
                     /gene="sapC"
                     /locus_tag="ACHOQW_000577"
     CDS             complement(8588..9478)
                     /gene="sapC"
                     /locus_tag="ACHOQW_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP9571707.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(9465..10430)
                     /gene="sapB"
                     /locus_tag="ACHOQW_000578"
     CDS             complement(9465..10430)
                     /gene="sapB"
                     /locus_tag="ACHOQW_000578"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP9571708.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(10427..12070)
                     /gene="sapA"
                     /locus_tag="ACHOQW_000579"
     CDS             complement(10427..12070)
                     /gene="sapA"
                     /locus_tag="ACHOQW_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP9571709.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(12383..12628)
                     /gene="ymjA"
                     /locus_tag="ACHOQW_000580"
     CDS             complement(12383..12628)
                     /gene="ymjA"
                     /locus_tag="ACHOQW_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFP9571710.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(12762..14144)
                     /gene="puuP"
                     /locus_tag="ACHOQW_000581"
     CDS             complement(12762..14144)
                     /gene="puuP"
                     /locus_tag="ACHOQW_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP9571711.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFAAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRKGMNK
                     SWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLY
                     EGDRTPVSET"
     gene            complement(14134..14298)
                     /locus_tag="ACHOQW_000582"
     CDS             complement(14134..14298)
                     /locus_tag="ACHOQW_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9571712.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(14447..15865)
                     /gene="puuA"
                     /locus_tag="ACHOQW_000583"
     CDS             complement(14447..15865)
                     /gene="puuA"
                     /locus_tag="ACHOQW_000583"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP9571713.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            16077..16841
                     /gene="puuD"
                     /locus_tag="ACHOQW_000584"
     CDS             16077..16841
                     /gene="puuD"
                     /locus_tag="ACHOQW_000584"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP9571714.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPERDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            16868..17425
                     /gene="puuR"
                     /locus_tag="ACHOQW_000585"
     CDS             16868..17425
                     /gene="puuR"
                     /locus_tag="ACHOQW_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP9571715.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            17575..19062
                     /gene="puuC"
                     /locus_tag="ACHOQW_000586"
     CDS             17575..19062
                     /gene="puuC"
                     /locus_tag="ACHOQW_000586"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP9571716.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            19064..20344
                     /gene="puuB"
                     /locus_tag="ACHOQW_000587"
     CDS             19064..20344
                     /gene="puuB"
                     /locus_tag="ACHOQW_000587"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP9571717.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYDARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            20382..21647
                     /gene="puuE"
                     /locus_tag="ACHOQW_000588"
     CDS             20382..21647
                     /gene="puuE"
                     /locus_tag="ACHOQW_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP9571718.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(21778..22755)
                     /gene="pspF"
                     /locus_tag="ACHOQW_000589"
     CDS             complement(21778..22755)
                     /gene="pspF"
                     /locus_tag="ACHOQW_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP9571719.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            22922..23590
                     /gene="pspA"
                     /locus_tag="ACHOQW_000590"
     CDS             22922..23590
                     /gene="pspA"
                     /locus_tag="ACHOQW_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP9571720.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            23644..23868
                     /gene="pspB"
                     /locus_tag="ACHOQW_000591"
     CDS             23644..23868
                     /gene="pspB"
                     /locus_tag="ACHOQW_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP9571721.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            23868..24227
                     /gene="pspC"
                     /locus_tag="ACHOQW_000592"
     CDS             23868..24227
                     /gene="pspC"
                     /locus_tag="ACHOQW_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP9571722.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            24236..24457
                     /gene="pspD"
                     /locus_tag="ACHOQW_000593"
     CDS             24236..24457
                     /gene="pspD"
                     /locus_tag="ACHOQW_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP9571723.1"
                     /translation="MNTRWQQAEQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            24532..24846
                     /gene="pspE"
                     /locus_tag="ACHOQW_000594"
     CDS             24532..24846
                     /gene="pspE"
                     /locus_tag="ACHOQW_000594"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP9571724.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            25056..26734
                     /gene="ycjM"
                     /locus_tag="ACHOQW_000595"
                     /pseudo
     CDS             25056..26734
                     /gene="ycjM"
                     /locus_tag="ACHOQW_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            26748..28040
                     /gene="ycjN"
                     /locus_tag="ACHOQW_000596"
                     /pseudo
     CDS             26748..28040
                     /gene="ycjN"
                     /locus_tag="ACHOQW_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            28061..28942
                     /gene="ycjO"
                     /locus_tag="ACHOQW_000597"
     CDS             28061..28942
                     /gene="ycjO"
                     /locus_tag="ACHOQW_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9571725.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            28929..29771
                     /gene="ycjP"
                     /locus_tag="ACHOQW_000598"
     CDS             28929..29771
                     /gene="ycjP"
                     /locus_tag="ACHOQW_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9571726.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            29802..30854
                     /gene="ycjQ"
                     /locus_tag="ACHOQW_000599"
     CDS             29802..30854
                     /gene="ycjQ"
                     /locus_tag="ACHOQW_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9571727.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            30872..31660
                     /gene="ycjR"
                     /locus_tag="ACHOQW_000600"
     CDS             30872..31660
                     /gene="ycjR"
                     /locus_tag="ACHOQW_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9571728.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            31670..32725
                     /gene="ycjS"
                     /locus_tag="ACHOQW_000601"
     CDS             31670..32725
                     /gene="ycjS"
                     /locus_tag="ACHOQW_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9571729.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            32722..34989
                     /gene="ycjT"
                     /locus_tag="ACHOQW_000602"
     CDS             32722..34989
                     /gene="ycjT"
                     /locus_tag="ACHOQW_000602"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP9571730.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            34986..35645
                     /gene="pgmB"
                     /locus_tag="ACHOQW_000603"
     CDS             34986..35645
                     /gene="pgmB"
                     /locus_tag="ACHOQW_000603"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9571731.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            35659..36741
                     /locus_tag="ACHOQW_000604"
     CDS             35659..36741
                     /locus_tag="ACHOQW_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9571732.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            36786..37691
                     /gene="ompG"
                     /locus_tag="ACHOQW_000605"
     CDS             36786..37691
                     /gene="ompG"
                     /locus_tag="ACHOQW_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP9571733.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(37802..38800)
                     /gene="ycjW"
                     /locus_tag="ACHOQW_000606"
     CDS             complement(37802..38800)
                     /gene="ycjW"
                     /locus_tag="ACHOQW_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9571734.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            38955..40352
                     /gene="ycjX"
                     /locus_tag="ACHOQW_000607"
     CDS             38955..40352
                     /gene="ycjX"
                     /locus_tag="ACHOQW_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP9571735.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            40349..41410
                     /gene="ycjF"
                     /locus_tag="ACHOQW_000608"
     CDS             40349..41410
                     /gene="ycjF"
                     /locus_tag="ACHOQW_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP9571736.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            41558..43099
                     /gene="tyrR"
                     /locus_tag="ACHOQW_000609"
     CDS             41558..43099
                     /gene="tyrR"
                     /locus_tag="ACHOQW_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP9571737.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(43143..43649)
                     /gene="tpx"
                     /locus_tag="ACHOQW_000610"
     CDS             complement(43143..43649)
                     /gene="tpx"
                     /locus_tag="ACHOQW_000610"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP9571738.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            43768..44733
                     /gene="ycjG"
                     /locus_tag="ACHOQW_000611"
     CDS             43768..44733
                     /gene="ycjG"
                     /locus_tag="ACHOQW_000611"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP9571739.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(44708..45436)
                     /gene="mpaA"
                     /locus_tag="ACHOQW_000612"
     CDS             complement(44708..45436)
                     /gene="mpaA"
                     /locus_tag="ACHOQW_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP9571740.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(45727..46365)
                     /locus_tag="ACHOQW_000613"
     CDS             complement(45727..46365)
                     /locus_tag="ACHOQW_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9571741.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(46386..47316)
                     /locus_tag="ACHOQW_000614"
                     /pseudo
     CDS             complement(46386..47316)
                     /locus_tag="ACHOQW_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(47316..48236)
                     /locus_tag="ACHOQW_000615"
     CDS             complement(47316..48236)
                     /locus_tag="ACHOQW_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9571742.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            48374..49273
                     /gene="pgrR"
                     /locus_tag="ACHOQW_000616"
     CDS             48374..49273
                     /gene="pgrR"
                     /locus_tag="ACHOQW_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP9571743.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT        12030 a        12303 c        13255 g        11873 t
ORIGIN      
        1 ctggttgtga taatacgcaa agtaacaatt catccccgtc agaaacagaa gtcggcgttg
       61 ttacagtcaa atctcaaccg gtctcggtag tcagtgaatt aaccggacgc accagtgctg
      121 cgctcagtgc cgaagtacgt ccgcaggttg ggggaattat ccagaaacgc ttatttaagg
      181 aaggtgatct ggtcaaggct ggacagccgc tctaccagat tgatgcggcc agttatcagg
      241 ctgcatggaa tgaagctcgg gcagcattac aacaagcaca ggcactggta aaagccgatt
      301 gccagaaagc gcagcgttat gcccgactgg tgaaagagaa cggtgtttca caacaggatg
      361 ctgatgatgc tcagtctacc tgtgcacaag ataaagccag tgtagcggcg aaaaaagccg
      421 cactggaaac tgcgcgcatt aatcttgact ggaccacggt aaccgcaccg atttcagggc
      481 gcattggcat ttcgtcggta acccctggcg cactggtgac cgcgtcgcaa gatacagcgt
      541 taacgactat tcgtggtctg gatacaatgt atgtcgacct cactcgctcc agtgtcgatt
      601 tattacgtct gcgtaaacag tcactggcga ccaacagtga caccatgagc gtctcactta
      661 ttctggaaga tggcacaacc tacagcgaaa aagggcgtct ggaactcacc gaagtcgctg
      721 tagatgagtc taccggttcg gtgacattac gggcgatttt ccccaatcca caacagcaat
      781 tattaccggg aatgtttgtt cgcgctcgtg tcgatgaagg cgtgatggaa gacgctattc
      841 tcgcgccgca acagggcgtc acgcgcgatg ctaaaggcaa tgcaactgcg ctggtggtga
      901 ataaagacaa taaagtagag cagcgaacgc tcgaaacggg agaaacatat ggtgataaat
      961 ggctggtgct aaacggcctg cacagcggcg accgactgat tgttgaaggt tctgccaaag
     1021 tcacttcagg gcagacggtc aaggctgttg aagttcaggc taatggaggc aacgcctgat
     1081 gttttcgcgc ttttttgttc gccgcccggt ctttgcctgg gttatcgcca ttttgattat
     1141 gttggcgggg attctggcta ttcgcacatt gcccgtcgcg caatatcctg acgttgcgcc
     1201 accgaccatt aaaatttcag ccacttatac tggtgcttct gccgaaacgc tggagaacag
     1261 cgtgactcag gtcatcgagc aacaactcac tgggcttgat aatttactct atttcagctc
     1321 aaccagcagc tctgatggtt cggtcagtat taatgtgacc tttgaacaag gtaccgatcc
     1381 agatactgca caggttcagg tgcagaataa aattcagcag gcggagtcgc gcctacccag
     1441 cgaagtgcag caaacgggtg ttacggtgga gaaatcacaa agcaactttt tgctgattgc
     1501 tgccgtgtat gacaccactg acaaagcctc cagttcggat atcgccgact ggttggttag
     1561 taacgttcag gacccgctgg cgcgtgttga aggtgtgggg agtctgcaag tctttggcgc
     1621 ggaatacgct atgcgcatct ggcttgaccc ggccaaactg gcgtcttact cgctaatgcc
     1681 ttcagacgtg caaagtgcta ttgaagcgca aaacgtgcag gttactgccg ggaaaatagg
     1741 ggcattgcct tcaccgaata ctcagcaact gaccgcaacg gttcgtgcgc agtctcgctt
     1801 gcagacggtg gaacagttca aaaatattat cgtgaaaagc cagtcagaca gcgcagttgt
     1861 tcgtataaaa gatgtggctc gcgttgagat gggcagtgaa gattataccg ctatcggcaa
     1921 acttaacggt cacccgtctg ccggggttgc tgtaatgctt tcgcccggtg cgaatgcgct
     1981 gaatacggcg acgctggtca aggataagat tgccgaattc cagcggaaca tgccgcaggg
     2041 atacgacatc gcttacccga aagacagcac tgaatttatt aaaatctctg tagaggacgt
     2101 aattcagacg ctgtttgaag ccatcgtatt ggtggtttgc gtgatgtatt tattcctgca
     2161 aaacctgcgt gccacactga ttccggcgtt agctgtcccc gtcgttttgc tgggcacatt
     2221 cggcgttctt gcgttgtttg gctattcgat taataccctg acgttgtttg ctatggtgct
     2281 ggcgatcggc ttgctggtgg acgatgccat tgtggtagtg gagaacgtcg aacgcattat
     2341 gcgtgacaaa gggttgcccg cgcgtgaagc cacggaaaaa tcaatgtgcg agatttctgg
     2401 cgcactggtt gccattgcgc tggtgttgtc agccgtattc ctgcctatgg ccttctttgg
     2461 gggatctacg ggggtaattt atcgtcagtt ttcgataacc attatctccg caatgctgct
     2521 ttccgtggtg gtggcattga ccttgactcc cgccctgtgc ggttccgtcc tccagcatgt
     2581 tccgccacat aaaaaaggct ttttcggcgc atttgaccgc ttttaccgcc gtactgaaga
     2641 taaatatcag cgaggcgtaa tttatgtcct gcgccgtgct gctcgaacga tggggcttta
     2701 tctcgtactg ggtggtggaa tggccctgat gatgtggaaa ctgccgggca gtttcttacc
     2761 cactgaagac cagggcgaaa tcatggtgca gtacacgctg ccggccgggg cgaccgctgc
     2821 ccgtacagca gaagtgaatc gccagattgt tgactggttc ctgattaacg agaaagcaaa
     2881 taccgatgtc atttttaccg ttgatggttt cagttttagc ggcagcggac agaacaccgg
     2941 gatggcgttt gtttcgttga aaaactggtc tcaacgtaaa ggggcagaaa acaccgccca
     3001 ggctatcgcc ctacgggcaa ccaaagagct gggcacaatt cgtgatgcca cggtatttgc
     3061 gatgacgccg ccagccgttg atgggctggg gcaaagcaat ggttttacgt ttgaattgtt
     3121 agctaacggt ggaaccgatc gcgaaacact actgcaaatg cgtaatcagt tgatagaaaa
     3181 agcgaatcaa agtccggagt tacattctgt tcgtgccaat gatttgccac aaatgccgca
     3241 attgcaggta gatattgata gtaataaagc ggtgtcatta gggttgagtt tgaatgatgt
     3301 caccgacacc ctgtccagtg cgtggggcgg tacttatgtg aatgacttta ttgaccgtgg
     3361 tcgtgtgaaa aaagtctaca ttcagggcga cagtgaattt cgttccgcgc cgtcagactt
     3421 aggtaaatgg tttgtgcgcg gtagtgataa cgccatgaca ccattctctg cttttgcgac
     3481 cacccgctgg ctgtatggac cggaaagact ggtgcgctac aacggttctg cagcctatga
     3541 aattcagggg gaaaatgcga ctggctttag ttccggcgat gcaatgacaa aaatggagga
     3601 actggcaaac agtcttcctg cgggaacaac ctgggcctgg agtggtttgt cattgcagga
     3661 gaaactggcc agcggtcagg cattgagtct gtatgcagtt tctattctgg tggttttcct
     3721 ctgccttgca gcactgtacg aaagctggtc agtcccgttc tcggtcattc tggtgatccc
     3781 tctggggctt cttggcgcgg cgctggcggc ctggatgcgt gatttaaaca acgatgttta
     3841 cttccaggtg gcgctattaa ccactatcgg cttgtcgtcg aaaaacgcca ttctgattgt
     3901 ggaatttgct gaagcggcgg ttgcagaggg ttattccttg agtcgtgcgg cattacgcgc
     3961 ggcgcagact cgtttacgcc caatcatcat gacctcgctg gcgtttattg cggggggtaa
     4021 tgccgctggc gatagcaacc ggctcagggg cgaacagccg catcgccatt ggtacgggca
     4081 ttattggcgg tacgctgacc gctacgttgc tggctatttt ctttgttcct ctgttttttg
     4141 tactggtgaa gcgtttgttt gccggtaaat cgcgccgtca ggagtaagtt atgttgcgtc
     4201 gttctcttat ttttctggtg ctgttgagtg cgggatgtgt ctcgctcgat ccccactaca
     4261 gcacgcctga atccccaatc ccggcgacct tgccgggagc acagggccag ggaaaggcaa
     4321 tcagccatga ctggcagcag gtgatccacg atccccgatt gcagcaggtg gtgactattg
     4381 cactgaacag taaccgcgat gtgcaaaaag cgattgctga tattgactca gcacgggcgc
     4441 tctatgggca aactaatgcg tcgttattcc cgacggtgaa tgcggcctta agttcaaccc
     4501 gcagtcgttc gctggcgaat ggtacggaaa ccactgccga ggctgacggc acggtgtcca
     4561 gttttacgtt agatctgttt ggtcgtaatc agagtttatc tcgggcggcg cgagaaacct
     4621 ggcttgccag tgaatttaca gcccagaaca cccgtttaac gctgattgct gaaatcagca
     4681 ccgcctggct gacactggcg gccgataaca gcaacctggc cctggcaaaa gaaacgatga
     4741 ccagtgcaga aaactcattg aaaattatcc agcgccagca acaggttggc acggcggcag
     4801 cgacagatgt cagtgaggcg atgagtgttt atcagcaagc gcgcgccagc gttgccagct
     4861 atcagacaca agtgatgcag gataaaaatg cgctgaactt actggcaggt acaacgcttg
     4921 aggaaaattt gctaccggga acactggaaa gcctgccgga acagatgata agtctggttc
     4981 ctgcgggagt gtcatctgac gttctgctgc gtcgcccgga tatccaggaa gcggaacata
     5041 atttgaagag cgccaatgcc gatattggcg cggcgcgggc taactttttc ccgacgattt
     5101 cgctgactgc cagcgctggt gttggcagtg acgcattgtc gtcgttgttc agccacggaa
     5161 tgcagatctg gtcatttacg ccatcggtca cattaccgct gttcactggc ggaagtaatc
     5221 tggcgcagct tcgttacgca gaagcacaaa agcgtggact cattgccacc tatgaaaaaa
     5281 ccgttcagag agcatttaaa gatgtcgcta acgcactggc gcggcggacc acgcttgaag
     5341 agcaactgga tgcacagcgc cagtatgtta aagctgagca acaaacggtc gatgttggct
     5401 tgcgtcgcta tcagacgggt gttggtgatt acctgaccgt gttgacggcg cagcgcagct
     5461 tgtggagcgc acagcaggag ctgctggcac tgcaactgac tgattttaca aaccgaatca
     5521 cgctctggca gtcgctgggc ggcggcatgt catcactcaa gtaaggaata atatggctag
     5581 agtctctctt tcatgggcat tgattcttgg tcttttagcc ggtatcggcc cgatgtgtac
     5641 cgatctttat ttgccggctt tgccggagat gtctgagcaa ctggcggcaa ccacgaccat
     5701 aacgcaatta acgctgactg catcactgat cggtcttggc gtcggacaac tgttatttgg
     5761 ccctctgagc gacaaaatag ggcgtaaacg tccgttaatc ttgtcgttgc tattgtttat
     5821 tgtttcttcc attttgtgcg cgacaacgaa caatatttac tggctggtgg tctggcgttt
     5881 tattcaaggg atcgcggggg cgggtggttc ggtgctctct cgttctattg ctcgtgacaa
     5941 atatcaggga gtaacgttga cccagttttt tgcgctgtta atgacggtga atggcctggc
     6001 accggtgttg tcgccagtgc tgggcgggta cattgttagc acttttgact ggcgcacttt
     6061 attctgggta atggctgaaa ttagcaccgt attgttgctg ggctgcgtgt tatttattaa
     6121 tgagaccttg ccagaaaata aaaggggctc atcattgcta ttaaccggac gaagcgtggt
     6181 gcagaaccgc cgttttatgc gcttttgcct gattcaaagt tttatgctgg caggcttgtt
     6241 tgcgtatatc ggctcttcgt cgttcgtgtt gcagaaggaa tttggcttta gtccaatgca
     6301 atttagcctg gtgtttggcc ttaacggcat cggacttatc attgcttcat ggatcttttc
     6361 gcgtctggcg cgacggatta atgcgatgac attgttgcga ggtggcctga tagcggcaat
     6421 tttgtgtgca ttgctcacgg tcttatgcgc atggacacaa ttgcccattc cggcactggt
     6481 ggcattattt ttcactattg cattttgtag cggcatcggc actgttggcg gggcagaggc
     6541 tatgagtgca gtagggacgc aggaatctgg aacggcgtct gcgttgatgg ggatgagcat
     6601 gtttgtcttc ggcggtatag ccgcgccatt gtcgggattt ggcggagaaa cactgttaaa
     6661 aatgagtctg gcaattacgg tgtgttatac actggcattc ctggttgctc tcaccagaat
     6721 cgacaatcaa aagtaatgaa tacctcatcc tgaggggggt tcccctgtca ggatgaggtg
     6781 cagaacacta acggtcctta cgccacgcat ccgccgtcaa tgcttcgcca aagtgcccgg
     6841 caatcagccg tttggtgagt tcatgcagcg gcgatgccag cacatccgcg gtgctgcctc
     6901 gctcgacaac ctcgccctga tgcatcacca gcacctggtc gctaatgtgc ttcatcattc
     6961 cgatatgctg ggtaacataa atatacgaaa tgccctgttt ttcctgtaat tccagcatca
     7021 gattaatcaa ctgcgaacgc atcgacatat ccagtgaagc gagggcttca tcggcaataa
     7081 tgacttttgg gcgcaatatc agcgcgcgcg ccagacccag acgctgtttt tgcccggggg
     7141 ccaacatatg cggatagtaa ctgacgtgat ccggtagcag cccaaccata cgcatcgttt
     7201 caataatctg tttgcgacgc tgttccggtt ccaggttggt gttcaggcgc agtggaaaat
     7261 ccagaatttg cgagatacgt tgacggggat tcaacgaggt cgaaggatcc tgaaaaatca
     7321 tgcgaatacg ctgactgcgg aaggaataat cgccaaaatg cagtggatga tcgtcaatca
     7381 ataactcgcc gctggtaggt tctatcattc ccgccagcat ttttgccagc gtggatttac
     7441 ccgaaccatt ctcgccaata atcgccagtg tctggccttc acgtagcgta aagctcaagg
     7501 gttttaccgc ttctacggtc tgacgacgaa accaaccggt ccggtagcgg aacgttttac
     7561 ttaaattacg cacttcaagc agcgtttcga tcatctcact ctttctccat gttcagcggg
     7621 aaatgacagg catagagatg atttttcgcc cccgtcaaac gtggcgtcac aatgcattct
     7681 cgttgtgcat acgggcaacg tggccccaga cgacaaccaa tcggtaactg ttcaagcagt
     7741 gggatagcgc cgggtagcgt attgaggcga cttttatgcg gcatcgcgct tccgaagtct
     7801 ggtatcgcgc ggatcagcgc ctgggtataa ggatgatgtg gcatcgtcac taactcctta
     7861 ctcggcgcgg tttccaccgt ttgaccgcag taaagcacgt taattttatc cgcccattgg
     7921 ctaagcattt gtaagtcatg gctgataagc aaaatagtgg tattgctgtt ttggtttaga
     7981 cgcgtcagca ggcgaaagat ttgcgcctgg gttgttggct ccattgagtt tgtcggttcg
     8041 tcagcaatca gcagacgcgg ttgattcgcc agtgcaatgg ctatcatcac tttctgacat
     8101 tcaccttcgg tcaactcata gggaaaactg cgcatcgcat ctttatgatc tttaattccc
     8161 acgcggtgca gcagttcaat ggcacggcgt ttgcgccagc caaaacgctg ccaccaacgg
     8221 cctttatagg tccaggctgg gatgttttgc atcaactggc gacccacacg ttctgaagga
     8281 tctagacacg actgcggttc ctggaaaatc atcgacacgt tgtggccaac cagtttgcgc
     8341 cgttcgcgtg cggagagacg cagcaaatcg atatcatcaa aacgcatacg gtctgcagta
     8401 acacgccagt tatctttatt caccccacaa atcgctttcg caatcaaact tttgccggaa
     8461 ccggattcac caacaagacc gcggatttca ccttcggtta aggtcatact tacccggtca
     8521 acggctttaa cccactcatc accggtttta aattcaatag tcaggttacg aatatcgagt
     8581 aatggcatta ttccaccccc gcaataattg cacgacggac accgtcgcca aggaggttaa
     8641 ccaacagcac gctaatcata attgccgcac ctggcagcat gacagtccac ggggcgacat
     8701 aaatcagttc cagcgcatca ccgagcatcg ctccccattc aggcgagggg agttgtgcgc
     8761 caagatcgag aaagcccagc gcggcgatat cgagaattgc catcgacagt gcgcgggtga
     8821 tctcggtgac cagcccggcg gtgatgttag gcatcacagc aaaccagaga atattcagcg
     8881 ttgatgcgcc atccagacgg gcggcgataa cgtactcttt ttccagttcg tcatgcacca
     8941 tgctgtaaat cgaacgtacc atacgcggca gcagcgccag ccagacagca aacatagcgt
     9001 gggacaaact cggtccggca aacgctacaa cgataattgc caacagcagg gaagggatcg
     9061 ccagtaaggt atccagaata tggttaagca ccgctgagcg gaggccgtgc gtcgccccgg
     9121 cgaaggtgcc cagtaccagg ccacagatcg tcgcggcaag tgtcaccaca aatgcgccac
     9181 ccacggttgg cgcagctccg ctcagtaaac ggcttaaaac atcgcgtccg aggtcgtcag
     9241 tccccaggaa gaaagaaact tcgccatagc gcgaccatga tggcggcaat aattggtaac
     9301 cgagaaattg ctggtcgatg ccgtaaggcg caaaccagcc gccaaacata cacagtacag
     9361 ccagccccgc gcagccgtac aggccgacca tcgcagaggc gtcactataa aatttgcgcc
     9421 aggcggtacg cagcgtgccc ggcgggcgct tttcgctgta tacgctatcg taaggcatac
     9481 cattccttat gtttcagagg gttagccatg gcacccaaaa tatcagaaat cacgttaaca
     9541 ataatgacca gtgagccaca caccatcact ccggcggaaa tggctgcgta atcctgctgg
     9601 cggatggcgt taattaacca gcgccctaaa cccggccagc taaaaaccat ttcggtgatc
     9661 atcgccagcg tcagcatggt ggaaaactgt aagcccaggc gaggaataac cggcggtaac
     9721 gcgttatgca gaacgtggcg acgcaaaata gtaaagcgcg acaaaccgcg ggtcgccgcc
     9781 gcttttacat agttctggtc atacacttcg atagtgctga tacgcatcag gcggatcact
     9841 tcagttgttg gcgcaaccga cagggtgatc acgggcaata tcatatggcg gattgcgctc
     9901 atgatcattt catcccgcca tggtgagtcc gaaagccagg catcaatcaa cgcaaaaccg
     9961 gtaatcggtt tcacttcgta gagcagatcg aaacgccctg aaacgggcaa ccaacctagc
    10021 gtgagtgaac aaaacagtgt caacagaagc gccagccaga acaccgggat tgaaaagccc
    10081 agtaaggcga tggcgttgat caagttatcc tgccacttat ggcgcgtaat cccggcaatc
    10141 atccccacgg ggatgccaac aatcagcgca aaaccgaagg cgaggatgca cagttccatc
    10201 gtcgccggga agacctcttt taactgttcg gcaattggtt gaccgttaat gctggaaaca
    10261 ccaaaatccc agtggatcag gccgttaaac cagaacaccc aggcattcca cagtgacgcg
    10321 ccttgtaacg gcgcgtgtgg agtgaaatag ctcaagctga agccaacgaa ggtcagcagg
    10381 aacaaggtga caatcaataa caaaatgcgg cgtaaggtga agataatcat ggttttttca
    10441 cctcatcctg tttctcgcga tacaccccag caaaggaggc gttaccaaac gggctaagta
    10501 ccagaccttt gatatcgtac cgataggcct gcaaacgcaa tgacgacgcc agcggcaaaa
    10561 tgggcaattc ctgcgccaga atactctgcg cttcgtcata ggcttcaata cgcgccgcca
    10621 gctgctgcga ggagagcgtc ttacgcaaca cgctgtcgaa tttcggatcg caccagtggg
    10681 cgaggttggt ctgtgaatga attgccgcgc agctcagtaa cggacggaag aaactgtccg
    10741 ggtcgttact gtccgtcgcc caaccggata acgtcagatc atggctcata tccatcaacc
    10801 gcgcctcctg aaagcgacct tctaccggca caatcaccac ttttacgcca acctgcgcca
    10861 tatccgcctg aatcagttcg gcagttttca gtggactggg gttccacgcc tgcgaacgtg
    10921 tgggcaccca cagtttcagc gttaaatttt ccagccccaa caacttcaac tgttcgcgcg
    10981 atttcgccgg attgtattca gtaattttag cctcgttgtc ataggcccac gaggcgcgcg
    11041 gtaaaataga ggccgccgtt tcagccgtac cataatagat ggattgcatc aggcgctggt
    11101 tattaatcgc cagtgccagc gcatggcgga cagcgggatt atttagcggc ggtttggcgg
    11161 tgttaaatgc cagataggcg acgttcatcc caggacgcag cgttaaacgc aagcgcgggt
    11221 cgtcacgcaa aatggatagc tggctggcag caggccaggc cagaacgtcg cattccccgg
    11281 tcaggagttt cgacagacgt ccggtgccgc cggagcctaa atccaccacc acctgcggca
    11341 ttaacggttt accgcgccag aagtcatcat gacgttgtag gcgaataaat tgcccggcgc
    11401 ggtattccga caactgatac ggtccagtgc cgaccggttg acggtcgagt tgctcctggc
    11461 gatcttcttt ctctaacttc cgggcatatt ctgccgacat gaccgaagca taatgggttg
    11521 cgaggtgcca caaaaaagaa gcatccggct gagccagacg gaactcaacg gtatgattat
    11581 ccagtttacg gacgcttttc acgttatcgg caaattgcag gctgtcgaag taggggaagt
    11641 tgctgccgtt gacgttatgc cacgggttgt tgcggtcaaa aattcgctgg aaggtaaaca
    11701 ccacatcgtc ggcattcatt ttgcgagtgg gagtaaacca ggcggttttt tgaaacggga
    11761 catcgcgacg caggtggaag cgataggtcg cgccgttgtc gagtacttcc cagctttcgg
    11821 caagttctgg catcaggcga taggtatagg gatcgacatc cagcagtcga tcataaaact
    11881 gggcggcaag ggtatcgaca attaacccac tgctcgcttt ggatgggtta aaggtgttga
    11941 cttgcccgct gacgcaatag acaaaaccgc tgtcgcggat atcagcatgc gggggggatt
    12001 caggcgcggc gattgcctga ccactcacaa gtccagcaat caccaaaaga gacgataata
    12061 cctggcgcat aatattaagg gattttatgt aaagaggcta tcttactaat atttaatgac
    12121 attttccatt accgtttgtg ttcaggggtc gtaatgaggg ccgtgtggtt ggtcgttgaa
    12181 cagatggcgt cgccatctgt tcaacattcg tacccgtgat ttctctataa ctataactca
    12241 cagaacaact tagcgaggag cagagcggta cgatcgtgag aaacccatta aagcacgctg
    12301 cagtgcgtgt cattgttagc cggatgcggc gtgaacgctt tatccggaca acgatactga
    12361 ccgatcgtct gcaattaaat aattactcat taccccattg attcaaaaac tctgcgatct
    12421 catcaatgcg tacgggatta atccccgctt cagcagccat ttcatgctgg gcttcttcgc
    12481 tgatctcttc attgttcatc aaacgggtga gcagtaactg gaagtagtgg gccagcggtg
    12541 tgcgttcagc gtctgcgaca ggctctgcgc attcggtcgc gtactcatcg gcaatatcaa
    12601 aatattttag cgggatatcg tggttcatta tttgcccctg gggttaatgc gctaagcata
    12661 agggcggatg atagcactcg tgatccgggt catacatctt tttatagtta atggcgcggc
    12721 gcattaccct caggcaggat aatgcggcgc gcatccgact attacgtttc gctcactggc
    12781 gttctgtcac cttcgtacag cggcactttg cgatagcggc ggatcaaata ccacaaatat
    12841 gcgccgccta acgaagccca taccagaccg agtgtcagtg acgttgactc gaggttaacc
    12901 cacagcacac ccaccgtcag cgcgccaacc agcggcatca gcaaatagtg gaagtgatcc
    12961 ttccagcttt tattcattcc tttacgccag aaatgattaa acaccgacag attcacgaag
    13021 gtaaacgcca ccagagcacc gaagttaatc aatgctgtcg cggtgactaa atcaaagaac
    13081 aatgccgaca tcgcgacaat tccgaccata atgacgttca gtgccggagt ccgccatttt
    13141 gggtgcacat agccaaacac gcgctccgga aacacattgt cgcgccccat cacatacagc
    13201 agacgtgaca cgctggcatg tgaggccagg ccagacgcta acgtgttcac aaacgtggtg
    13261 cagaggaaaa tcgactggaa cagcttaccg ccgacgtaga gtgcaatttc aggcagtgcg
    13321 gcgtccgggt ctttaaagcg gctgatatcg gggaagaaca gctgcataaa gaacgacgcc
    13381 gcgataaaga taacgccacc atagaccgcc gtcaggaaga tggctttcgg gatcacgcgt
    13441 gcggcgtctg gtgtctcttc cgaaagtgtg gtcaccgcat cgaaaccgag gaacgagaaa
    13501 cagacaatcg tcgccccggt aataatcggg atcaggtgcg cgttctcgct gataaacggc
    13561 tgaagtgacc agacggtgcc aacgccttct cctttatgca gtccctgaac caccaggaag
    13621 ataaacacca ccatgatgga gatttgcacc agtacaaaca gggtattgaa gttagcgacc
    13681 aggttgacgc tcttcagatt cgcggcggtt aaaatggcga cgaaggttac cacccacacc
    13741 cacggcggca cttccgggaa gagggcggag agatagattt tcgccaacaa aacgttgatc
    13801 atcggcaaaa agagataatc cagcagtgat gaccagccga ccataaatcc gacgtgcggg
    13861 ttaatcgact tttgcgcgta ggtataggcc gaaccggcct ccggaaactg gcgaaccagt
    13921 ttgccgtagc tgatagcggc aaacagcacg cccgccagcg ccagcaaata ggacgcaggg
    13981 acgtgaccgt cgctaatgcc agatacaatg ccaaaggtat cgaacacagt catcggcgtg
    14041 agataggcca gacccatcat cacaacctgc cacaatttca gtgattttcg cagacgggtt
    14101 ttgccgggtt gcgcagcaat attcagtggt gaattaatag ccataattgc tgcctcccct
    14161 gcgtaataag gtttgcggtc gtgtcgacct gaaatcaaac ctgtttagca agggaagcaa
    14221 agggggcgga gtccagtcag tcgcaggagg ggcgcatacg cagcctcccg cgccatgcgg
    14281 ggatttaatc atcggcatca tctcatttcc tcgtcacagt cttaatcgtt aacggaagcc
    14341 cgttgctgcc tgcgctccat aacgcgacat ccgggaaatg tctggattac cagcaaatgt
    14401 caggcctggc tccgctcagg ccgatgaaac aaccccgcaa ggggtactat gcgtttttca
    14461 acatccactc aatttctgtt tctgtgatga ggcgctcaaa ctgcaacagc tcatcatttt
    14521 tacaggcgtg atacacatgg caaaaccgtt cgcctaaata gcggcgcagg tgatcattct
    14581 cgataaactc acccagggca tcgctctggc gaatcgggaa gggtaagcct tcctgttcca
    14641 gcccgttacc ttcgacttct tcctgcaacg gcagctcgtt atcaaggcca tgcaaaatac
    14701 cggcaaaaat cgctgccatc accagatatg ggttggcatc ggcacccgcc acgcgatatt
    14761 ccacgcggtg attatgacga tcgccgcatg gaatacgcag ggcgacggta cggttattat
    14821 ggccccacga cgcctgcgtc ggtacataca tccccggctg gaagcggcga tacgagttca
    14881 cgtttggtgc cagcaacgcc attgatgacg gcatcaggtc aatcatcccg gcgagcatct
    14941 ttttcagcag cggcgaatct tcgccttctg tgtcagaaag cacgttctcg ccacgattat
    15001 tttgcatact gatatggata tgcattccgc tgcccgcgtg ctcttcatac ggcttcgcca
    15061 taaaagtggc gtgcatctta tgcttttctg ccatcagacg caccaggcgt tttagtgcca
    15121 gcgcatcatc gcaggcttcc agcacgttat cagtatggta aaggttgatt tcaaactgac
    15181 ccggcgaggc ctcagcgacc gcgccatctg ccggaatcag ctgtaactgc gccagttcat
    15241 caatatcatt gagcacgtcg gcgaagtggt tgaggttatc aacggagtaa acctggcttt
    15301 gtgtattgcg gtcatcagtg ccgggcgcgc agggcggttg cagataccct tcagcgtcgc
    15361 gctggcgatc cagtaaatag aactccagct ctaccgctac gaccgggaac aatccgcgct
    15421 ggcgcagctg ctgccacagg cggttaagaa cgttccgcgg ctcaacgtca aagggagcgc
    15481 catcttcatc gaccatggtc aggagcatct gaccgataaa ctctggatcg gcggcagaag
    15541 gggttaagga accgagaaca ggaacacagg tacgatccgg ctcgcccatt tcctgaccca
    15601 gacctgcttc ttctactacg ttgcccagaa tatccattgc aaataccgag gccgggaaat
    15661 aacacccttt ctcgagcttc ttcaggcttg aaacaggaat acgttttcca cggaagcaac
    15721 cattcaaatc ggtaagtaaa acatcaacat attgcgtatt cgggtagcgc tccaggtagc
    15781 gtttcacttc ctgcgtaaag gcgctacccc gcctctcttc tgactgctga acaaagttct
    15841 ctacttcaac gatattggtt tccatgattc ttcgcctttg gtttgttttc cgctcgttat
    15901 caaagcgtaa aatataatga ccaccattcg aatctgtatg caaactaaat gtttgtcaaa
    15961 tgttaaattg agtttgcaaa aatgaaaacc cactgctaga ttgaaaaaat attgaacata
    16021 aaggtcattt aaaccgcagt aatggcgata atttagtcca ctttgtgaga ttgagaatgg
    16081 aaaatataat gaacaatccg gttatcggtg tcgtaatgtg caggaacagg cttaaggggc
    16141 atgcgaccca gactctgcaa gaaaagtacc tgaatgccat catccacgca ggcggcttgc
    16201 caattgcgct gccacatgcg ctggcggaac cgtcgttact tgaacaactt ttgccgaaac
    16261 tcgatggcat ttaccttcct ggtagtccca gcaatgtgca gccgcaccta tatggtgaaa
    16321 acggcgatga gcctgacgcc gatcccgagc gtgatcttct gagcatggcg ttaattaatg
    16381 ccgcactcga aaggcgcatc cccattttcg ccatctgccg gggtttacag gaactggtgg
    16441 tggcaaccgg tggttcgttg catcgcaagc tgtgcgaaca gcctgaattg ctggaacatc
    16501 gggaagatcc cgaactgccg gtggaacagc aatatgcacc gtcgcatgaa gttcaggttg
    16561 aagagggggg attgctgtct gcattgttac ctgaatgtag caacttttgg gtaaactctc
    16621 tgcatggaca aggggcgaag gtcgttagcc cacggttgcg tgttgaagct cgctcgccgg
    16681 atggcttagt tgaggcggtt agtgtcatca atcatccttt tgcgctgggc gtacagtggc
    16741 acccggaatg gaacagtagc gagtacgcgc tttcgcgtat attgttcgag ggctttatca
    16801 ccgcttgtca gcaccatatc gctgaaaaac agcgactctg accactacag tttaaggaaa
    16861 tgcaaatatg agtgatgagg gactggcgcc aggaaaacgc ttgtcggaaa tccgccagca
    16921 gcaggggctt tcacaacgtc gtgccgccga actctccggg ctgactcaca gtgctatcag
    16981 tacgatagaa caagataaag tcagccctgc catcagtacg ctgcaaaagc tgctgaaggt
    17041 gtatggtctg tcactctcgg aattcttttc cgagccggaa aaacctgatg agccgcaggt
    17101 cgtcattaat caggacgatt taattgagat gggcagtcag ggtgtgtcaa tgaagctggt
    17161 tcataacggt aacccgaatc gcacgctggc gatgatcttt gaaacgtacc agccgggcac
    17221 aaccactggg gaaagaatta agcatcaggg tgaggaaata ggcactgtac tggaaggtga
    17281 aattattctg acgattaatg gtcaggatta ccacctcgtc gcggggcaaa gttatgccat
    17341 taataccggc atcccgcaca gtttcagtaa tacgtcggca ggtatttgcc gaattatcag
    17401 cgcccatacg cccaccacgt tttaatcttt ttgttctgta agccgggtaa gcgcaatgcg
    17461 cccggcaatc cataaagaat gtcgccagaa ataaacgggc atacggcccg gggatctctg
    17521 cgccctgacg ttcacaaact gcatatatct gatagacgtg aaacaggagt cataatgaat
    17581 tttcatcatc tggcttactg gcaggataaa gcgttaagtc tcgccattga aaaccgctta
    17641 tttattaacg gtgaatatac tgctgcggcg gaaaatgaaa cctttgaaac cgttgatccg
    17701 gttacccagg caccgctggc gaatattgcg cgcggcaaga gcgtcgatat cgaccgtgcg
    17761 gtgagcgcag cacgcggcgt atttgaacgc ggcgactggt cactctcttc tccggcaaaa
    17821 cgtaaagcgg tgctgaataa actcgccgat ttaatagaag ccaacgccga agagctggca
    17881 ctgctggaaa ctctcgacac cggcaaaccg attcgtcaca gtctgcgtga tgatataccc
    17941 ggcgcggcgc gcgccattcg ctggtacgcc gaagcgatcg acaaagtgta tggcgaagtg
    18001 gcgaccacca gtagccatga gctggcgatg atcgtgcgtg aaccggtcgg cgtgattgcc
    18061 gccatcgtgc cgtggaactt cccgctgctg ctgacttgct ggaagcttgg cccggcgctg
    18121 gcagcaggga acagcgttgt cctgaaaccg tctgaaaaat caccgctcag tgcgattcgt
    18181 ctcgcggggc tggcgaaaga agcaggcttg ccggatggtg tgttgaacgt ggtgacgggt
    18241 tttggtcatg aagcggggca ggcgctgtcg cgtcataacg atatcgacgc cattgccttt
    18301 accggttcga cccgtaccgg gaaacagctg ctgaaagatg caggcgacag caacatgaaa
    18361 cgcgtctggc tggaagcggg cggcaaaagc gccaacatcg ttttcgctga ctgcccggat
    18421 ttgcaacagg cggcaagcgc caccgcagca ggcatcttct acaaccaggg gcaggtgtgc
    18481 atcgccggaa cgcgcctgtt gctggaagag agcatagccg atgaattctt agccctgtta
    18541 aaacagcagg cgcaaaactg gcagccgggc catccacttg atcccgcaac caccatgggc
    18601 accttaatcg actgcgccca cgccgactcg gtccatagct ttattcagga aggcgaaagc
    18661 aaagggcaac tgttgttgga tggccgtaac gccgggctgg ctgtcgccat cggcccgacc
    18721 atcattgtgg atgtagaccc gaatgcgtcc ttaagccgcg aagagatttt cggtccggtg
    18781 ctggtggtca cgcgtttcac atcagaagaa caggcgctac agcttgccaa cgacagccag
    18841 tacggccttg gcgcggcggt atggacgcgc gacctctccc gcgcgcaccg catgagccga
    18901 cgcctgaaag ccggttccgt cttcgtcaat aactacaacg acggcgatat gaccgtgccg
    18961 tttggcggct ataagcagag cggcaacggt cgcgacaaat ccctgcatgc ccttgaaaaa
    19021 ttcaccgaac tgaaaaccat ctggataagc ctggaggcct gaaatgacag aacataccag
    19081 cagttactac gccgccagtg cgaataaata cgcgccattc gacacgctga atgagtcaat
    19141 ttcctgcgac gtttgcgtgg ttggcggcgg ctataccggg ctgtcttccg cactgcatct
    19201 ggcggaagcg ggctttgacg tggtggttct ggaagcctca cgcatcggct ttggcgccag
    19261 cgggcgcaac ggcgggcagc ttgtgaactc ctacagccgc gacatcgacg ttatcgaaaa
    19321 aagctacggc atggacaccg cccgtatgct cggcagcatg atgttcgaag gtggtgagat
    19381 catccgcgaa cgtatcaaac gttatcagat tgactgcgac taccgccccg gcggtctgtt
    19441 tgtggcgatg aatgataaac agctcgccac acttgaagag cagaaagaga actgggaacg
    19501 ctacggcaat aaacagctgg agatgctgga cgccaacgcc attcgccgcg aggtcgccag
    19561 cgatcgctac actggtgcgc tgctggatca cagcggcggg catattcacc cgctgaacct
    19621 cgccattggc gaagcggacg ctatccgcct gaacggcggg cgcgtgtatg agctttccgc
    19681 agtgacgcag atccagcaca ccacgcccgc cgtagtgcgt acagccacag gtcaggtgac
    19741 ggcgaagtat gtgattgtcg ccgggaatgc gtacctgggc gataaagtgg agccggaact
    19801 ggcgaaacgc agcatgccgt gcggtacgca ggtgatcacc accgagcggc tttccgatga
    19861 tttagcccgt tcgctgatcc cgaaaaacta ctgcgtggaa gactgtaatt atctgcttga
    19921 ttactaccgt cttaccgccg acaaccgttt gctgtacggc ggcggcgtgg tgtacgacgc
    19981 gcgcgacccg gatgacgttg agcgtcttgt ggtgccgaaa ctgctgaaaa ccttcccgca
    20041 gttgaagggc gtgaaaatcg actatcgctg gacgggcaac ttcctgctga ccctgtcgcg
    20101 tatgccgcag ttcggtcgcc tcgataccaa catctattac atgcagggtt acagcggcca
    20161 cggcgtcacc tgtactcatc tcgccggacg tttgattgcc gaactgctgc gcggcgacgc
    20221 cgaacgtttc gatgccttcg ccaatctgcc gcattacccg ttccccggcg ggcgcacgct
    20281 gcgcgtaccg tttaccgcga tgggcgcggc gtattacagc ctgcgcgatc gtctgggcgt
    20341 ttaatttccg attaacagtg aagagtcaaa aggtgtgaaa catgagcaac aatgaattcc
    20401 atcagcgtcg tctttctgcc accccgcgcg gggttggcgt gatgtgtaac ttcttcgccc
    20461 agtcggctga aaacgccacg ctgaaggatg ttgagggcaa cgagtacatc gatttcgccg
    20521 caggcattgc ggtgctgaat accggacatc gccaccctga tctggtcgcg gcggtggagc
    20581 agcagctgca acagtttacc cacaccgcgt atcagattgt gccatatgaa agctacgtca
    20641 ccctggcgga gaaaatcaac gcccttgccc cggtgagcgg gcaggccaaa accgcgttct
    20701 tcaccaccgg tgcggaagcg gtggaaaacg cggtgaaaat cgcccgcgcc cataccggac
    20761 gccctggcgt gattgcgttt agcggcggct tccacggtcg tacgtatatg actatggcgt
    20821 taactggaaa ggtcgcgccg tacaaaatcg gcttcggccc gttccctggt tcggtgtatc
    20881 acgtacctta tccgtcagat ttacacggca tttcaacaca ggactccctc gacgccatcg
    20941 aacgcttgtt taaatcagac atcgaagcga agcaggtcgc ggccattatt ttcgaaccgg
    21001 tgcagggcga gggcggtttc aacgttgcgc caaaagagct ggttgccgcc attcgccgcc
    21061 tgtgcgacga gcacggtatt gtgatgattg ccgatgaagt gcaaagcggc tttgcgcgta
    21121 ccggcaaact gtttgcgatg gatcattacg ccgataagcc ggacttaatg acgatggcga
    21181 aaagcctcgc gggcgggatg ccgctttcgg gtgtggtcgg taacgcgaat attatggacg
    21241 cgcccgcgcc gggcgggctt ggcggcacct acgccgggaa tccgctggcg gtggctgccg
    21301 cgcacgcggt gctcaacatt atcgacaaag agtcactctg tgagcgcgcc atgcagttgg
    21361 ggcagcgtct gacgaaaacc ctgattgatg ccaaagaaaa cgttccggcc atcgcggcgg
    21421 tacgtggtct gggctcgatg attgcggcag aatttaacga cccgcaaacg ggcgagccgt
    21481 cagccgccat tgcgcaaaaa attcagcaac gcgcgctggc gcagggactg cttctactga
    21541 cctgtggcgc atacggcaac gtgattcgtt tcctgtatcc gctgaccatc ccggatgcgc
    21601 aattcgatgc ggcaatgaaa attttgcagg atgcgctgag agattaatac gcagaagcag
    21661 gggcaacgca ataatgcctg atatgctgcg cttatcagac ctgcaaacga cgtattgatt
    21721 atgtatgccg gataaggcgt tcacgccgca tccggcaagt tgtattgccc aacttcgcta
    21781 aatctggtgc tttttcaaca acgcgcggaa ctgatgatag gttaagccca gcaattcagc
    21841 tgcgcgcttc tggttatatt tcccttgttg caaactgagt tgcagcaact ctttttcctg
    21901 ctgcatctga aactcacgta aatccagcgg cagtgttgga agcgaggtgg tttctgaaac
    21961 ggcgatagcg tcttcaggcg gacgccgttt aaagggatca ataatgatgt catcaagcgg
    22021 ataatcgctg gtgccgtggc gatacactga acgttccacc acgtttttca attcacgaat
    22081 atttcctggc caacgataat tcagcaatgt ttctctggcg cgctccgtaa accccgggaa
    22141 cagaggcagc ttgatttccc gacacatctg gatggcaaag tgttccgcca ttaacataat
    22201 gtcgctttca cgttcgcgta gtggcggcag ttgcactaca tcgaaagcca gccggtcgag
    22261 caggtcagcg cgaaaagtac cttcattgac catcgccggg agatcggcat tcgtcgcgca
    22321 taccaaccgc acattcacct gcaacggctg gctgccgcca acgcgctcca gttcaccgta
    22381 ctcaatcacg cgcaataatt tctcctgcac catcatcggt gccgtagcga gttcatcaag
    22441 aaatagcgta ccgccatcgg cacgttcaaa tctccctgga tgacgttttt gcgcaccggt
    22501 aaacgccccc gcttcgtgac caaacagttc ggaatccagc agattttcat ttaacgccgc
    22561 gcagttaagg gaaataaacg gcccttgcca acgggaggag agataatgca ggcggctggc
    22621 gatcagctct ttaccggtgc cgcgttcgcc gatgatgagc accggtttgt ccagcggtgc
    22681 gagatgcgaa acctgttcca gcacttcgag aaagctgttc gcctcaccga gtaaattatc
    22741 tttgtattct gccatgatga aattcgccac ttgttagtgt aattagctaa ttcatcctgg
    22801 catgttgctg ttgattcttc aatcagatct ttataaatca aaaagataaa aaattggcac
    22861 gcaaattgta ttaacagttc agcaggacaa tcctgaatgc agaaatcaag aggacaacat
    22921 tatgggtatt ttttctcgct ttgccgacat cgtgaatgcc aacatcaacg ctctgttaga
    22981 gaaagcggaa gatccacaga aactggtgcg tctgatgatc caggagatgg aagatacatt
    23041 ggttgaagta cgttctactt cggcgcgtgc gctggcagaa aagaaacagc tgactcgccg
    23101 tattgaacaa gcgtcggcgc gtgaagttga atggcaggaa aaagccgaac tggcgctgct
    23161 gaaagagaga gaggatttgg cgcgtgcggc gttaattgaa aaacagaaac tgaccgatct
    23221 gattaagtcc ctggaacatg aagtgacgct ggtggacgat acgctggcac gcatgaagaa
    23281 agagattggc gagctggaaa acaaattgag cgaaacacgc gctcgccagc aggcattgat
    23341 gttacgccat caggcggcaa actcgtcgcg cgatgtgcgt cgtcagctgg acagtggcaa
    23401 actggatgaa gcaatggctc gtttcgaatc tttcgaacgt cgtattgacc agatggaagc
    23461 ggaagcagaa agccacagct tcggtaaaca aaaatcgctg gacgatcagt ttgccgaact
    23521 gaaagccgat gatgcaatca gcgagcaact ggcacaatta aaagccaaaa tgaagcaaga
    23581 caatcaataa taacatccag gcggcgtccg aacgcgccgc cgctcatcgt ctaaggagta
    23641 cttatgagcg cgctatttct ggctattcca ttaaccattt ttgtgctgtt tgttttaccg
    23701 atctggttat ggctgcatta cagcaatcgt tctggtcgca gtgaattgtc gcaaagtgag
    23761 cagcagcgat tagcgcaact ggctgatgaa gcaaaacgga tgcgcgaacg tattcaggcg
    23821 ctggaatcta ttcttgatgc agaacatccg aactggaggg atcgctaatg gcgggcatta
    23881 atctcaataa aaaattatgg cgtattccac agcagggcat ggtccgtggc gtctgcgccg
    23941 ggattgccaa ctattttgat gtaccggtaa aactggtgcg tatcctggtg gtgttgtcga
    24001 ttttcttcgg tctggcgctg tttaccctgg ttgcttacat cattttgtca tttgcgcttg
    24061 atccaatgcc ggacaacatg gcctttggtg agcagctacc ttccagcagt gaattgctgg
    24121 atgaagtcga ccgtgaactg gcggcaagtg aaacgcgttt acgcgagatg gaacgttatg
    24181 tcacttccga tacattcacg ttacgtagcc gtttccgtca actgtgagga aagttatgaa
    24241 tactcgctgg caacaggccg agcaaaaggt aaagcctggt ttcaaattag caggcaagct
    24301 ggtacttctt accgcactgc gctatggccc agcgggtgtg gcgggctggg cgataaaatc
    24361 agttgctcgc cgaccgctga aaatgttgct ggctgtggcg ctggaaccgc tgttaagtcg
    24421 ggctgccaat aaactggcgc agcgttataa aaggtgaggg gagtttcgca aaaattgtta
    24481 aatctcaggc gtataatgga tagcaatatt catccataga aggacgctta catgtttaaa
    24541 aaaggcttac ttgctctggc actggtgttt tcactgcccg ttttcgccgc tgaacactgg
    24601 atcgatgttc gtgttccaga gcagtatcag caagagcacg ttcagggggc catcaatatt
    24661 cccctgaagg aagtgaaaga gcgcattgcc accgtcgttc cggataaaaa cgacaccgtg
    24721 aaagtgtatt gcaatgccgg acgccaatca gggcaggcaa aagagatcct tagcgagatg
    24781 ggatataccc acgttgagaa cgccggtggc ctgaaagaca tcgcaatgcc gaaggtcaaa
    24841 ggttaaaaga attcaccatg agcggcgctt atgcgccgtt tttttctgtc acaccttatt
    24901 tacatcccta tagattattt gcgtcagctc acaaatacgc tttttccctg gtaaaaaatg
    24961 atttcctgcg tgactaaaac ccttgtgctc aattgacagt ttattttctg cggagtagtc
    25021 tctcatttca tgggaccgct accaggaaag gcaacatgaa acagaaaatt acggattacc
    25081 tggacgaaat ctacggtgga acatttaccg caactcattt acagaaactt gtaacgcgtc
    25141 ttgagagtgc gaaacgatta attacacagc gacgtaaaaa acactgggat gaaagtgatg
    25201 tcgtgttaat tacctatgcc gatcaatttc acagtaatga tttaaaacca ctacccacat
    25261 ttaatcagtt ttaccatcaa tggctgcaaa gcattttttc acatgttcat ttattacctt
    25321 tttatccatg gtcatctgat gatggatttt cggtaattga ttatcatcag gtcgccagtg
    25381 aagcgggtag tggcaggata ttcagcagct cggtgaatgc agtcatttaa tgtttgattt
    25441 tgtctgcaac catatgtcgg caaaaagtga atggtttaaa aactatttac aacagcagcc
    25501 aggttttgaa gattttttta ttgcggttga tccgcaaacc gatctcagcg ctgtcacccg
    25561 tccgcgtgca ttaccgctat taacgccgtt tcaaatggat gataattcca cgcgccattt
    25621 atggaccacc tttagcgacg atcaaattga cctgaattac cgtagccctg acgtgttgct
    25681 ggcgatggtg gatgttttgc tttgttacct ggaaaagggc gcagaatatg tccgtctgga
    25741 tgccgttggc tttatgtgga aagagccggg aacaagctgc atccatctgg aaaaaacaca
    25801 tctgattatc aaactgttac ggtcgattat tgatgacatc gcgccaggta cggtgattat
    25861 tactgagacg aatgttccgc ataaagataa cattgcttac tttggcgaag gcgatgacga
    25921 agcgcatatg gtgtaccagt tctcgctgcc gccgctggtg ttacatgcgg tgcaaaaaca
    25981 gaacgtcgag gcgctgtgcc agtgggcgca aagtctgagc ttgccctccg gtaaaacgac
    26041 ctggtttaac ttcctcgcct ctcacgatgg catcgggctg aacccactgc ggggcttgtt
    26101 accagaaagc gaaatattag cgctggtcga ggcattacag caggaaggtg cattagtaaa
    26161 ctggaaaaat aatcccgacg gtacgcgcag tccctatgaa ataaatgtca cctatatgga
    26221 tgcgttaagc cgccgcgaga gtagcgatga agaacgttgc gccaggttta tccttgccca
    26281 tgcgattttg ttaagtttcc ccggtgtgcc agcgatatat attcaaagta ttctgggctc
    26341 gcgtaatgat tacgcaggtg tcgaaaaact cggatataac cgtgcgatta accgtaaaaa
    26401 atatcacagt gaagagataa cccgagaact gaacgatgaa gctacattaa ggcatgcggt
    26461 atatcatgag ttgtcgcgtt taattacact tcgtcgcagc cataacgagt ttcatccgga
    26521 taataatttt accatcgata cggttaattc atccgtaatg cgtattcaaa gaagtaacgc
    26581 agagggtaat tgtctgaccg gattgtttaa tgtcagtaaa aatattcagc atgtaaatat
    26641 tactggcctg cacggtcggg atctgattag tgaagttgat atattgggta ataaaataac
    26701 gctgcgcccc tggcaggtta tgtggattaa ataaaaagga acatctcatg attaaatcaa
    26761 aaatcgtgct gttatcagca ctggtttcct gcgccctgat ttcaggctgt aaagaagaaa
    26821 ataaacccca tgtatccatc gaatttatgc attcttcggt ggagcaggag cgtcaggcag
    26881 ttatcagcaa attgattgcc cgttttgaaa aagaaaaccc aggtatcacc gttaaacaag
    26941 tgcccgtcga agaggacgct tataacacca aagtcatcac tctttcacgt agcggctcgc
    27001 tgccggaagt gattgaaacc agtcatgact acgccaaagt tatggacaaa gagcagctta
    27061 tcgatcgcaa ggcggttgcc acggtcatca gcaacgttgg tgaaggcgcg ttttacgatg
    27121 gcgtactgcg tattgtgcgt accgaagatg gtagcgcatg gaccggtgtt cctgtcagcg
    27181 cctggattgg cggcatctgg tatcgcaaag atgtgctggc aaaagcgggg ctggaggagc
    27241 cgaaaaactg gcaacagctg ctggacgttg cacagaaact gaatgacccg gcgaataaaa
    27301 aatacggtat tgcgctgcct acggcagaaa gcgtgctgac ggaacaatcc ttctcccagt
    27361 ttgcgttatc caactaggcc aacgtcttta acgccgaagg caaaatcacc cttgattcac
    27421 cagagatgat gcaggcgctg acctattacc gcgaccttgc tgccaacacc atgccgggtt
    27481 ctaacgacat catggaagtg aaagacgcct ttatgaacgg caccgcgccg atggcgattt
    27541 actccaccta tatccttccg gctgtgatta aagaaggcga tccgaaaaac gtcggtttcg
    27601 tggtgccaac cgagaaaaac tctgccgtct acggcatgtt gacctcgctg accattaccg
    27661 ccggacaaaa gactgaagaa acggaagcgg ctgaaaaatt tgtcaccttt atggagcagg
    27721 cagacaacat tgccgactgg gtgatgatgt cgccaggcgc ggcgctgccg gtgaacaaag
    27781 cggttgtcac tacttccacc tggaaagaca acgacgttat taaggcgctg ggtgaactgc
    27841 caaatcagtt aatcggtgaa ctgccgaata ttcaggtctt tggcgcagta ggggataaaa
    27901 aatttacccg catgggcgat gtgacggggt ctggggtggt gagctccatg gtgcataacg
    27961 tcaccgtagg taaagccgat ctccctggca cgctgcaaac gagccagaaa aagctggatg
    28021 aactgatcga acagcactaa acccaggaca ggaatccgca atgaacaggc ttttttcagg
    28081 tcgttccgat atgccctttg cgctgttgct tctcgcaccc agcttattac tgctgggcgg
    28141 tctggttgcg tggccaatgg tgtcgaatat cgaaattagt tttttacgtc tgccgctcaa
    28201 tcccaacatc gagtcaacgt ttgttggggt aagcaactat gtgcgtattc tctccgatcc
    28261 cggcttctgg cactcgctgt ggatgacggt ctggtatacc gcgttggtgg tggcgggcag
    28321 caccgtgctc gggctggcag tggcgatgtt tttcaaccgc gagttccgcc tgcgcaaaac
    28381 cgcgcgttcg ctggtgatcc tctcctacgt aacgccgtct atttcgctgg tgttcgcctg
    28441 gaaatacatg ttcaacaacg gctacggcat tgtgaactac ctcggcgtcg atcttctgca
    28501 tctttatgag caggcaccgc tgtggttcga taatccgggc agtagttttg tgctggtggt
    28561 gatcttcgcc atctggcgct acttcccgta tgcctttatc tcgtttctgg cgattttgca
    28621 gaccatcgac aaatcgctgt acgaagcggc agagatggat ggcgctaatg cctggcaacg
    28681 gtttcgtatc gtcacgctgc ccgcgattat gccggtcctg gcgacggtgg tgacccttcg
    28741 caccatctgg atgttctaca tgttcgcgga tgtttatctg ctgacaacca aggtcgatat
    28801 tcttggtgtc tatctctaca aaaccgcctt tgcctttaat gacttaggga aagcggcggc
    28861 gatctcggtg gtgctcttca tcattatttt cgctgtcatt ctgctgacca ggaaacgggt
    28921 gaacctcaat ggcaacaaat aaacgcacac tcagtcgtat cggtttttac cgcgggctgg
    28981 cgctgtttct catcatcacg ctgtttccgt tttttgtgat gctgatgacc tcgttcaaga
    29041 gcgcaaaaga ggcgatctcg ctgcatccta cgctgctgcc gcaacagtgg acgctggagc
    29101 attatgtcga catttttaac ccggtgattt tcccgtttgt cgactacttc cgtaacagca
    29161 tggtggtgtc ggtggtttca tccgtggtgg cggtatttct cggcattctc ggagcttacg
    29221 cgctttctcg cctgcgcttt aaaggccgga tgaccatcaa cgccagcttt tacacggtgt
    29281 atatgttctc cggcattttg ctggtggtgc cgctgttcaa aattatcacc gcacttggca
    29341 tttacgacac cgagatggcg ctgatcatta ccatggtgac gcaaactctg cccaccgccg
    29401 tgtttatgct gaaaagttac ttcgacacca tccctgatga gatcgaagaa gcggcgatga
    29461 tggatggcct caaccgtctg caaattatct tccgtatcac cgtgcccctg gcgatgtccg
    29521 ggctgatatc cgtattcgtc tactgcttta tggtggcatg gaacgactat ctgtttgcgt
    29581 cgattttcct ctccagcgcc agcaatttca ccttaccagt cggcctgaac gcgctgttca
    29641 gtacgccaga ttacatctgg ggacggatga tggcggcctc gctggtgacc gcattgccgg
    29701 tggtgattat gtatgcgctt tcggaacgtt ttattaagag tggtttgacc gccggtggcg
    29761 tgaagggcta aagcggccag tttctttaca aggagtttta catgaaaaag ttagtagcca
    29821 cagcaccgcg cgttgctgcg ctggttgagt atgaagatcg ggcgatttta gccaatgaag
    29881 tgaagatccg cgtgcgtttc ggtgcaccga aacacggaac ggaagtggtc gacttccgcg
    29941 ccgccagccc gtttatcgat gaagacttta acggcgaatg gcagatgttc accccgcgtc
    30001 cggcagatgc gccgcgcggc attgagtttg gcaaattcca gcttggcaac atggtggttg
    30061 gcgacattat cgagtgcggc agcgacgtta ccgactacgc ggtgggcgac agcgtatgcg
    30121 gctacggccc gctctccgag acgatcatca ttaacgcagt gaataactac aagctgcgca
    30181 aaatgccgga aggcagctcc tggaaaaacg ctgtctgcta cgacccggcg cagtttgcca
    30241 tgagtggcgt tcgcgatgcc aacgtacgcg taggggattt tgtagtggtg gtagggcttg
    30301 gcgcgatcgg tcaaattgcc atccaactgg ctaaacgcgc tggcgcgtcg gtggtaattg
    30361 gcgtcgatcc tattgcccat cgttgtgata ttgctcgtcg tcacggtgcg gatttctgcc
    30421 ttaatcccat tggcactgac gtaggcaaag agatcaaaac gctgaccggc aagcagggtg
    30481 ccgatgtgat tatcgaaacc agcggttacg ccgacgcgct gcagtcggcg ctgcgcggcc
    30541 tggcttacgg tggcaccatc tcctatgtcg cgtttgctaa accgtttgct gaaggtttta
    30601 acctcggacg cgaagcgcat ttcaataacg ccaaaattgt cttctctcgc gcgtgcagtg
    30661 aaccgaaccc ggattatccg cgctggagcc gtaagcgtat tgaagaaacc tgctggaaac
    30721 tgctgatgaa cggttatctc aattgcgaag atttaatcga cccggtagtg acctttgcca
    30781 acagcccgga aagctacatg cagtatgtcg atcagcatcc ggaacagagc atcaaaatgg
    30841 gcgtcacgtt ttaattcaaa ggaatgaaaa aatgaaaata ggcacacaga atcaggcgtt
    30901 cttcccggaa aatattctgg agaaatttcg ttatatcaaa gagatgggct tcgatggttt
    30961 tgagattgac ggcaaactgc tggtcaacaa catcgaagaa gtcaaatcgg cgatcaaaga
    31021 aaccggttta ccggtgacca ccgcctgcgg cggctatgac ggctggattg gtgactttat
    31081 cgaagagcgt cgtcttaatg gcttaaagca gatcgaacgc attctcgaag cgctggcaga
    31141 agtggggggt aaaggtatcg tcgttccggc agcgtggggc atgtttacct tccgcttacc
    31201 gccgatgacc tcgccgcgta gcctggacgg cgaccgcaaa atggtgagtg attccctgcg
    31261 cgtactggaa caggtcgccg cgcgtaccgg aaccgtggtg tatctcgaac cgttaaaccg
    31321 ctatcaggat catatgatca acaccctcgc cgatgctcgt cgttacagcg tcgaaaacaa
    31381 tcttaaacat gtgcagatta tcggcgattt ctatcacatg aacatcgaag aagataacct
    31441 ggcgcaggcg ctgcatgaca accgcgactt gctcggtcat gtgcatattg ccgataacca
    31501 tcgttaccag ccgggcagcg gcaccctcga tttccacgcg ctgtttgaac agctgcgcgc
    31561 cgataactat cagggttatg tggtgtatga agggcgtatc cgggcagaag atcctgccca
    31621 ggcgtaccgt gattcgttga cctggttgcg tacctgctaa gaggtctttg tgaaaagtgc
    31681 aatgacaagc tctccactgc gggtcgcgat aataggcgca ggccaggtgg cggataaggt
    31741 tcatgcttcg tactactgca cccgcaacga tctggaactg gtggctgtct gtgacagccg
    31801 cctttcccag gcgcaggcgc tggcagaaaa atacgggaat gcatccgtgt gggacgatcc
    31861 gcaggccatg ctgctggcgg tgaaacctga tgtggttagc gtctgctcac ctaaccgttt
    31921 tcattacgaa cataccctga tggcgctgga agcgggctgc catgtgatgt gcgaaaaacc
    31981 gcccgccatg acgccagaac aggcgcggga aatgtgcgat accgcgcgca aacagggcaa
    32041 ggtgctggcc tacgactttc accatcgttt tgcgctcgat acgcaacagc tgcgtgaaca
    32101 ggtgaccaac ggcgttttgg gagagattta cgttaccacc gcccgcgccc tgcgtcgctg
    32161 cggcgttccc ggctggggtg tctttaccaa taaagaactg cagggtggtg gcccgctgat
    32221 cgacatcggc attcatatgc tggatgctgc gatgtatgtg ctgggctatc ctgcggtgaa
    32281 aagcgtgaat gcgcatagct ttcaaaagat cggcacgcaa aagagctgtg gtcaatttgg
    32341 cgagtgggat ccggcaactt acagcgtcga agattcgctg tttggcacca ttgaatttca
    32401 taacggcggc attctgtggc tggaaacgtc atttgcgctc aacatccgcg aacagtcgat
    32461 tatgaacgtc agcttttgtg gtgataaagc cggtgcgacg ctgtttccag cacatatcta
    32521 caccgataac aacggtgaat taatgacgct gatgcaacgg gaaatggcag acgacaaccg
    32581 ccatttgggc agcatggaag cctttatcaa tcacgtacag ggcaagcccg tgatgatagc
    32641 cgacgccgag caggggtaca tcatccagca actggtggcg gcgttatatc aatccgcaga
    32701 aacagggacg cgtgtggaat tatgaccagg ccagtaacgt tatcagaacc ccatttcagc
    32761 cagcataccc tgaacaagta tgcatcgctg atggcgcagg ggaacggcta tcttgggctt
    32821 cgcgccagcc atgaagaaga ttacacgcgc cagacgcgag ggatgtatct ggcggggctg
    32881 tatcatcggg cgggaaaagg tgaaatcaac gaactggtga acctgcctga tatcttgggg
    32941 atggagattg ccataaatgg tgaggttttc tcgttatccc acgaagcctg gcagcgtgag
    33001 cttgactttg ccagtggcga attacgccgc aacgttgtct ggcgtaccag caacggcgca
    33061 ggttacacca tcgccagccg tcgctttgtt tcggcagacc aactgccgct cattgcgctg
    33121 gaaatcacta ttacgccact ggacgccgac gcgtcagtgc tgatttcaac aggtatcgac
    33181 gccacgcaaa ccaaccacgg tcgccaacat ctcgacgaaa cccaggtgcg ggtgtttggt
    33241 cagcatctga tgcaggggat ctacaccacc caggatggac gcagtgatgt ggccatcagc
    33301 tgttgctgta aggtgagcgg tgatgtgcag caatgctata ccgccaaaga gcgccgtttg
    33361 ctgcaacata ccagtgcgca gcttcatgca ggcgagacag tgacgttgca aaaactggtg
    33421 tggatcgact ggcgggatga caggcaagcc gttttagacg agtggggcag cgcgtcgctt
    33481 cgccagcttg aaatatgcgc gcagcagagt tacgaccaac ttcttgcagc atcaacagaa
    33541 aactggcgtc aatggtggca gaaacgtcgt atcacggtaa atggcggcga tgcgcacgat
    33601 cagcaagcgt tagattatgc gctttatcat ctgcgcatca tgacgccggc tcacgacgag
    33661 cgcagcagta ttgcggcaaa aggcttaacc ggcgaaggct acaaaggcca cgttttctgg
    33721 gatacagaag tatttttgct gccgttccat ctgtttagcg atccgacggt tgcccgaagt
    33781 ttactgcgtt atcgctggca caacttgcca ggcgcgcagg agaaagcacg gcgcagcggc
    33841 tggcagggcg cgctatttcc gtgggaaagc gcgcgcagcg gcgaagaaga gacgccagaa
    33901 tttgccgcca ttaacattcg taccgggctg cggcaaaaag tggcctcggc gcaggcggaa
    33961 catcatctgg tggccgatat cgcctgggcg gttattcaat actggcagac cacgggggat
    34021 gaaagtttca ttgctcatga aggcatggcg ctacttctgg aaactgcaaa gttctggatt
    34081 agccgcgcgg tgagggttaa cgaccgtctg gaaattcatg atgttattgg gccagacgaa
    34141 tataccgaac atgtcaataa taacgccttc accagctata tggcccgcta caacgttcaa
    34201 caggcgctga atattgcccg ccagttcggc tgtagcgacg atgcgtttat ccatcgcgcc
    34261 gaaatgttcc tcaaagagct atggatgcca gaaacgcagc ccgatggcgt tttgccgcag
    34321 gatgattcgt ttatggctaa gccggcgatt aatctggcta aatacaaagc ggcggcgggg
    34381 aagcaaacca ttctgctgga ttattcacgc gcagaagtga acgagatgca gatcctcaaa
    34441 caagctgatg tggtgatgct caattacatg ctgccggagc agttctcagc ggtatcgtgt
    34501 cttgccaatc tgcaatttta tgaaccgcgc actattcacg actcgtcatt aagtaaagca
    34561 atccacggca ttgttgccgc acgctgtggc ctgctgaccc aaagttatca gttctggcgc
    34621 gaggggactg aaatcgatct tggtgctgat ccgcatagtt gtgatgatgg tatccacgct
    34681 gccgcaactg gcgctatctg gctgggggcg attcagggtt ttgccggggt gagcgtgcgt
    34741 gacggtgaat tacatctcaa tccggcgtta ccggagcagt ggcaacagtt gtctttccct
    34801 ctgttctggc agggctgcga attacaggtc acgctcgacg cgcagcgtat tgcgattcga
    34861 acttctgcgc ccgtttcact gcgtttgaac gggcagctta tatccgtggc tgaagaatct
    34921 gttttctgtt tgggtgattt tattttgccc ttcaatggga ccgctaccac gcatcaggag
    34981 gatgaatgaa actgcaaggg gtaattttcg atctggatgg tgtaatcacc gataccgcgc
    35041 atctgcattt ccaggcgtgg cagcagattg ccgctgaaat tggcatcagc attgatgcgc
    35101 agtttaacga atccctaaaa gggatcagcc gcgatgagtc tctgcggcgc attctgcaac
    35161 acgggggcaa agagggcgac tttaacccgc aggagcggac gcaactggcg tatcgcaaaa
    35221 atctgcttta cgtccactca ctgcgcgagc tgacggttaa tgctgtgctg cccggtattc
    35281 gcaatttgct ggcagagctc cgcgcacagc agatcccggt agggctggct tctgtctccc
    35341 tgaatgcgcc gacgatttta gcggcgctgg agctgcgcga gtttttcacc ttctgcgcgg
    35401 atgcttccca acttaaaaac tcgaaaccgg atccggaaat ctttctcgct gcctgtgccg
    35461 ggctgggcgt gccgccgcag gcatgtatcg gcattgaaga tgcgcaggcg ggcattgacg
    35521 ccattaacgc cagcggtatg cgctcggtgg ggatcggcgc aggcttaacc ggggcgcaat
    35581 tattgttgcc ctcaacggac tccctgacct ggccgcggtt atcggccttc tggcaaaacg
    35641 tatagcaaag gaatcaacat ggctcagctt tcgttacaac atattcaaaa aatctacgat
    35701 aaccaggtgc atgtggtgaa ggacttcaac ctggaaattg ccgataaaga gttcatcgta
    35761 tttgtcggcc cgtcgggctg cggtaaatcg accaccctgc gcatgattgc cgggcttgag
    35821 gagatcagcg gcggcgatct gttgatcgac ggcaaacgaa tgaatgacgt tccagccaaa
    35881 gcacgcaata tcgcaatggt gttccagaac tacgcgttgt atccgcatat gacggtttac
    35941 gacaacatgg cgtttggcct gaagatgcaa aaaatcgcca aagaggtgat tgatgagcgg
    36001 gtgaactggg cggcgcaaat tctcggcctg cgtgagtacc tgaagcgcaa gccgggcgcg
    36061 ctttccggcg ggcaacgcca gcgtgtggcg ctcgggcggg cgattgtgcg cgaagcgggc
    36121 gtgtttttaa tggatgaacc gctctctaac cttgatgcca agctgcgcgt acaaatgcgc
    36181 gcagagatca gcaagctgca tcagaaactg aacaccacca tgatctacgt gacccacgat
    36241 cagaccgaag cgatgaccat ggcgacgcgg attgtgatta tgaaagacgg gattgttcag
    36301 caagtgggtg cgccgaaaac cgtttataac caacccgcga atatgtttgt tgccggattt
    36361 attggatcac cagcgatgaa ttttattcgc ggcacgatcg atggcgataa attcgttacg
    36421 gaaacgctta aattaaccat tcccgaagag aaattagcgg ttctgaaaac acaggaaagt
    36481 ttgcataagc ccatcgtgat gggaatgcgc ccggaagata ttcatccgga cgcgcaagag
    36541 gaaaataata tttccgccaa aattagcgtg gtagaattaa ccggtgcgga atttatgctc
    36601 tacaccacgg ttggggggca cgagttagtg gtccgtgctg gtgcgttaaa tgattatcat
    36661 gcaggagaaa atatcactat tcattttgat atgactaaat gtcatttctt tgatgcagaa
    36721 acggaaatag caattcgcta aatacagggg gaaggcattc ccccaggata atacaaggaa
    36781 caataatgaa aaagttatta ccctgtaccg cactggtgat gtgtgcggga atggcctgcg
    36841 cacaggccga ggaaaagaac gactggcact ttaatatcgg cgcgatgtac gaaatagaaa
    36901 acgtcgaggg ttatggcgaa gatatggatg ggctggcgga gccttctgtc tattttaatg
    36961 ccgccaacgg gccgtggaga atttctctgg cctattatca ggaagggccg gtagattata
    37021 gcgcgggtaa acgtggaacg tggtttgatc gcccggagct ggaggtacat tatcagttcc
    37081 tcgaaagcga tgatttcagt ttcggcctga ccggcggttt ccgtaattat ggttatcact
    37141 acgttgatga accgggtaaa gacacggcga atatgcagcg ctggaaaatc gcgccagact
    37201 gggatgtgaa actgactgac gatttacgtt tcaacggttg gttgtcgatg tataaatttg
    37261 ccaacgattt gaacacgacc ggttacgctg atacccgtgt cgaaacggaa acaggtctgc
    37321 aatatacctt caacgaaacg gttgccttgc gagtgaacta ttatctcgag cgcggcttca
    37381 atatggacga cagccgtaat aacggtgagt tttccacgca agaaattcgc gcctatttgc
    37441 cgctgacgct cggcaaccac tcggtgacgc cgtatacgcg cattgggctg gatcgctgga
    37501 gtaactggga ctggcaggat gatattgaac gtgaaggcca tgattttaac cgtgttggtt
    37561 tattttacgg ttatgatttc cagaatggtc tttccatttc gctggaatac gcgtttgagt
    37621 ggcaggatca cgacgaaggc gacagcgata aattccatta tgcaggtgtc ggcgtaaatt
    37681 actcgttctg ataatgggct aaattgccgg atgcggcgcg agtactttat ccgatctata
    37741 aatgtaggcc ggataagatg cgccagcatc gcatccggca ttcaggcaag gtcgctggta
    37801 tttatttcag cgtcatatgc gtggcaacgg taatattttg tggtgacggt tttccggcaa
    37861 ttaagcggaa taataactca cagctttgtc gacctaactc ctgcgtcgga acatcgatgc
    37921 cgcccggtgc aggcgttaaa ataaatgaca gcgtttcatt gctataaccc accaccgcta
    37981 actgctgcgg aatagcaatg tttttctctg ccgccgcacg ataaatgctc attaatttca
    38041 ggctgtcagt ggcaaacact gcctcaggca acggtgactg acttaataat tgccgtgctg
    38101 cttgcagtgc tgtttcatgg gtataaccgc catcaacaat ccattcatca cgcactgcaa
    38161 tattatgatc ttccagactc tgcttataac catttacccg atccactgaa acatggacat
    38221 caagcggtgc atgcagacag gcaatatttt gatgtccact ttcaattaac gcatcggtca
    38281 acgcaatgct gtcaccaaaa ttatcggtat cgacagaata aacatgggca tattgacctt
    38341 caactttgcc aatcaccacc acaggaatat cgtatttatc aagttgggca aaaaatgact
    38401 catccgctgg cgaacttagc ataataatgc ctttaatcat tttctgctta attttgcttt
    38461 cacatttttg taagtcttct gccggggtgt gcgacgtctg caatatcacg tcgaaacctt
    38521 cttcttcagc tttggcggtg atggcatgca aaacttcaga gaaaaacgga ttacccgccg
    38581 tagttttggt cgaacgggta gaaatcacca taatggcatc aaaacccgaa gaggtcagcg
    38641 cgcgggccag cttgtttggt tgatactgta attcttcaat ggcccgtaac actttttcgc
    38701 gcgcttccgg ggagatattg gtttgcttat tcagcacgcg tgatacggtg gattttgata
    38761 cgcctgcaac cctggcaata tcataaatag taggggacat aggtcaggaa ctccgtccga
    38821 acgtgaatgc tgcacatctt atggagttcg cccgtcgatg acaacacggg tagttgataa
    38881 tcaatggcct ggcccccaca ttcatatcct tatgaatgat ttttttctgg tcttcagagc
    38941 agcacaggac agcaatgaag cgacttaaaa atgaacttaa tgcgctggtg aatcggggtg
    39001 tcgacagaca tctgcgcctc gccgtaaccg gacttagccg cagcggcaaa acggcgttta
    39061 tcactgcgat ggtcaatcag ttgctcaata ttcatgccgg agcacgtttg ccgctattaa
    39121 gtgcggtgcg tgaagagcgt ctgctgggcg taaaacgcat tcctcagcgt gactttggca
    39181 ttccgcgttt tacctacgac gaagggttgg cgcagctgta tggcgatcct cccgcctggc
    39241 cgacgccaac gcgcggcgtc agcgaaattc gcctggcact acgctataaa tcgaacgatt
    39301 cgctgctgcg ccactttaag gatacctcca cgctgtatct ggagattgtg gattaccctg
    39361 gcgaatggtt gctcgacctg ccgatgctgg cgcaggacta tttaagctgg tcgcgccaga
    39421 tgacgggctt actcaatggt cagcgcggcg aatggtcggc gaaatggcga atgatgtgcg
    39481 aagggctgga cccgctagcg cctgccgacg aaaaccggct ggcagacatt gccgccgcgt
    39541 ggaccgatta tctccaccac tgtaaacagc aggggctgca ctttattcag cctgggcgct
    39601 ttgtcttgcc gggggatatg gcaggtgcgc ccgcgctgca attcttcccg tggccggatg
    39661 tcgatgcctg gggcgagtcc aaactggcgc aggccgataa gcacaccaat gccggaatgc
    39721 tgcgcgagcg gtttaattat tactgcgaga aggtggtgaa ggggttctat aagaatcatt
    39781 ttctgcgctt tgaccgccag attgtgctgg tggattgcct gcaacctctc aacagtgggc
    39841 cacaggcatt taatgatatg cgtctggcgc tgacgcagct gatgcaaagt ttccactatg
    39901 ggcagcgtac cctgttcagg cgtttgtttt cgccggttat cgataagcta ttgtttgctg
    39961 ccactaaagc ggaccatgtg accatcgatc agcacgccaa tatggtttca ttgctgcaac
    40021 aactaattca ggatgcctgg caaaatgcgg cgttcgaagg gatcagcatg gattgcctgg
    40081 ggctggcgtc agttcaggcg accaccagtg gcattattga tgttaacggt gagaaaatcc
    40141 cggcgctgcg cggtaatcga cttagcgatg gcgcaccgct cactgtttat cctggcgaag
    40201 ttcccgcacg tttgcctggt caggcgttct gggataagca agggttccag tttgaagcgt
    40261 ttcgcccgca ggtgatggat gtcgacaaac cgctgccgca tattcgtctt gatgccgcgc
    40321 tggaattttt aataggagat aaattgcgat gaccgaaccg ttaaaaccac gtattgattt
    40381 cgacggtccg ctggaggtcg aacagaatcc aaaattcagg gcgcagcaga cctttgacga
    40441 aaatcaggcg caaaattttg ccccggccac gctcgacgaa gcgcaggaag aagaggggca
    40501 agtcgaagcg gtaatggacg cagcgttacg tccgaaacgc agcctgtggc gcaaaatggt
    40561 gatgggcggg ctggctctgt ttggcgcaag cgttgtcggg cagggtgtac agtggacaat
    40621 gaatgcctgg caaactcagg actgggtggc gctgggtgga tgtgctgctg gggcattgat
    40681 tatcggcgct ggcgtaggtt ctgtggtaac agagtggcgg cgcttatggc gcttgcgaca
    40741 gcgcgcccat gaacgcgacg aagcgcgcga tttgttgcac agccacggca cgggcaaagg
    40801 ccgcgcattt tgcgaaaaac tggcgcagca ggcgggtatt gatcagtctc atccagcgct
    40861 gcaacgctgg tatgcctcaa tccatgaaac gcagaacgat cgtgaagtgg tcagcctgta
    40921 tgctcatctg gtccagccgg ttttagatgc ccaggcgcgg cgcgaaatca gccgctcagc
    40981 agctgaatca acgttgatga ttgcggtcag cccgctggcg ctggtggata tggcatttat
    41041 cgcctggcgc aatctgcgtt tgattaatcg catcgccacg ctgtatggca ttgaactggg
    41101 atattacagc cgtttgcgcc tgttcaagct ggtattgctg aatatcgctt tcgccggagc
    41161 cagcgaattg gtgcgcgaag tgggaatgga ctggatgtcg caagatctcg ctgctcgttt
    41221 gtctacccgc gcagctcagg ggattggtgc aggacttctg acggcacgac tggggattaa
    41281 agctatggag ctttgccgcc cgctgccgtg gattgacgat gacaaacctc gcctcgggga
    41341 tttccgtcgt cagcttatcg gtcaggtgaa agaaacgctg caaaaaggca aaacgcccag
    41401 cgaaaaataa tgcaatatcg ggtgctgacc ggatatcttt acgccgaagt gcccgttttt
    41461 ccgtctttgt gtcaatgatt gttgacagaa accttcctgc tattcaaata gtgtcatatc
    41521 atcatattaa ttgttctttt ttcaggtgaa ggttcccatg cgtctggaag tcttttgtga
    41581 agaccgactc ggtctgaccc gcgaattact cgatctactc gtgctaagag gcattgattt
    41641 acgcggtatt gagattgatc ccattgggcg aatctacctc aattttgctg aactggagtt
    41701 tgagagtttc agcagtctga tggccgaaat acgccgtatt gcaggtgtta ccgatgtgcg
    41761 tactgtcccg tggatgcctt ccgaacgtga gcatctggcg ttgagcgcgt tactggaggc
    41821 gttgcctgaa cctgtgctct ctgtcgatat gaaaagcaaa gtggatatgg cgaacccggc
    41881 gagctgtcag ctttttgggc aaaaattgga tcgactgcgc aaccataccg ccgcacaatt
    41941 gattaacggc tttaattttt tacgttggct ggaaagcgaa ccgcaagatt cccataacga
    42001 gcatgttgtt attaatggac agaacttcct gatggagatt acgcctgttt atcttcagga
    42061 tgaaaatgat caacacgtcc tgacaggtgc ggtggtgatg ttgcgatcaa cgattcgtat
    42121 gggccgtcag ttgcaaaatg tcgccgccca ggacgtcagc gccttcagtc aaattgtcgc
    42181 tgtcagcccg aaaatgaagc atgttgtcga acaggcgcag aaactggcga tgctaagcgc
    42241 gccgctgctg attacgggtg acacaggtac aggtaaagat ctttttgcct acgcctgcca
    42301 tcaggcaagc ccaagagcga gcaaacctta cctggcgctg aactgtgcgt ctataccgga
    42361 agatgcggtc gagagtgagc tgtttggtca tgctccggaa gggaagaaag gattctttga
    42421 gcaggcgaac ggtggttcgg tgctgttgga tgaaataggt gagatgtcac cacggatgca
    42481 ggcgaaatta ctgcgtttcc ttaatgatgg cactttccgt cgggttggcg aagaccatga
    42541 ggtgcatgtc gatgtgcggg tgatttgcgc tacgcagaag aatctggtcg aactggtgca
    42601 aaaaggcatg ttccgtgaag atctctatta tcgtctgaac gtgttgacgc tcaacctgcc
    42661 gccgctacgt gactgtccgc aggacatcat gccgttaact gagctgttcg tcgcccgctt
    42721 tgccgacgag cagggcgtgc cgcgtccgaa actggccgct gacctgaata ctgtacttac
    42781 gcgttatgcg tggccgggaa atgtgcggca gttaaagaac gctatctatc gcgcactgac
    42841 acaactggac ggttatgagc tgcgtccaca ggatattttg ttgccggatt atgacgccgc
    42901 aacggtagcc gtgggcgaag atgcgatgga aggttcgctg gacgaaatca ccagccgttt
    42961 tgaacgctcg gtattaaccc agctttatcg caattatccc agcacgcgca aactggcaaa
    43021 acgtctcggc gtttcacata ccgcgattgc caataagttg cgggaatatg gcttaagcca
    43081 gaagaagaac gaagagtaag cgcgaatatg cctgatggtg caacaccatc aggcatatta
    43141 acttatgctt tcagtacagc cagagctgct tcgtaatccg gctcggtggt gatttcatcc
    43201 accagctggc tgaaaatcac attgtcattt tcatcaataa ccacaacggc acgcgctgcc
    43261 agacctttca gtgggccatc agcaattgcc acaccgtagg cttgcagaaa ttcagcgtta
    43321 cggaaagtgg agagggtgat aacgttgttc agaccttctg cgccgcagaa acgagactgg
    43381 gcgaacggca gatcggcaga gatacacagc acaacggtgt tgtcgatctc ggttgccagt
    43441 tggttaaact tacgtactga tgcggcgcaa acaccggtat caatactcgg gaaaatgttc
    43501 agcactttgc gtttacccgc aaactgaccg agggtgacgt cagacagatc ttttgccacg
    43561 agagtaaaag tctgcgcttt gctacccgcc tgcgggatgg aattggcgac tgtaaccggg
    43621 ttgccctgga aatgaacggt ttgtgacatg attatctttc ctgtttacat atagttaacg
    43681 tcacacctag tttatgctaa ctgtcaataa cacagcaaac gctatttgcg cttaatccgc
    43741 agaccaccgc gacaccaagg agtaaagatg agaaccgtta aggtatttga ggaagcctgg
    43801 cccttacata ccccgtttgt gattgcccgg ggaagtcgca gtgaagcgcg cgtggtggtg
    43861 gttgaactgg aagaagaggg tattaaaggc accggcgaat gcacgccgta tccgcgttat
    43921 ggagaaagtg atgcctcggt aatggcgcaa attatgagcg tcgtgccgca attagagaaa
    43981 gggctgacac gggaggagtt gcaaaaaatt ctccctgccg gcgcagcacg taatgcgctg
    44041 gattgtgcat tgtgggatct ggccgcgcga aaacagcagc aatcgctggc tgatttgatc
    44101 ggcataacgc ttcccgagac agttatcact gcacagacgg tagtcatcgg tacgcctgat
    44161 cagatggcca atagtgcatc aacactctgg caggccggcg cgaaattact gaaagtgaag
    44221 ctggataacc atcttatcag tgagcggatg gtggcaattc gcacagctgt gcccgatacc
    44281 acgctgatcg ttgatgcaaa tgaatcctgg catgcagaag ggttggcggc acgttgccag
    44341 ctattggcgg atttaggcgt tgcgatgctt gaacaaccgc ttcctgcgca ggacgatgcg
    44401 gcgctggaga attttattca tccgttgccg atttgtgctg atgaaagttg tcatactcgt
    44461 agcaatttga aggcgctgaa agggcgctat gagatggtta atattaagct cgataaaacc
    44521 gggggtctga cggaagcgct ggcgctggcg actgaagcgc gtgcacaagg tttcagtctg
    44581 atgctgggct gcatgttgtg tacctctcgg gccattagcg ccgctttacc gctggtgccg
    44641 caggtcagtt tcgccgatct tgacggaccg acctggctgg cggtagatgt ggaaccggcg
    44701 cttcagttca cgacgggcga attgcatctt taggatgcca gcgcagcaag tttgccatcg
    44761 caaagagata tttttcgctg gcttcgtcgg aggagatggg cggaaactct gcggtgatgc
    44821 aatgcaagtt cagatctgca caccaactgc cgaaagagcc aggcgtttca taaccgacgc
    44881 tggttaccag aggcaattca aacgcctgag ccagccattc gcctaattcg ctgtgtctgg
    44941 gatcttcaat acaggccagt ggatcgtgga atgagaccac ccaggcaggt tgaatgcggt
    45001 gtataagctg gcacagcgcc tgggtttcag gttcggagcc aggtttgtca cctgtcagta
    45061 atacgacatc acgttcttca gcggcgctgt tccagcgata aaccgtttca ccttccttcc
    45121 agttcgccgc cggaaagttt ctgtttaaat ccacaccatt agcattggcc cgtaacccca
    45181 gctggcagcc gtcaggattc acacacagca ccacatgatg gcggcgcaaa gaaggtgtca
    45241 aagtccgcag agcacaggag agcgtgacga ccgaagagtt ttcatcaccg tgagtgccag
    45301 ccagaatcaa accactttcg cgactggctg caggggccgg aaaccagatt aacggcgcgc
    45361 ccaataatga acgtccgtaa tgttctgttc cgggcggaaa tgcgccgcgt tcggcgcgtg
    45421 ggcgggttac ggtcatgatc gtctctggtt caggaagtat tacaggcagt gttgtgcaaa
    45481 ttttgcggat tatcaaatcc ttttcttcaa cgctatgtca tcgatcttgt atcacttttt
    45541 tgccggaagt tgtttgtatt ttcattgatg tgcagatatt agaattgtcg taatgttata
    45601 tacatggcgt attttattct tactggttct acacatgtta atcgcatctg attgatcagt
    45661 tcaccttaat aagtttaata aatcgtactg cacatagtac agtacgatgg tggttatata
    45721 attgatttac atgaagaatg gcttgtcgcc atcagtcccg ggttgattga tgcctatatt
    45781 ctcaccaatg tgtttctcag gtttatcgat gatatctgta attaatgctg cgacactctt
    45841 aggtgatacg atcgtacctt tgaatggttc attgcgtgag gtaagctcat aatcaataat
    45901 atcttcatcc gtcagccagg cagggcgaag aattgtatat tccagtccag aagcctcaat
    45961 tgcatccgca gccctgcgga atggttttag gggctcacca ataactgcat tattccattc
    46021 gccaaatttt cctggaactt catcataaat gccgagggaa agaacaaaga tcagacgttt
    46081 aacatcacag gctttcatcg cagcgatcac tgaatttgcc tgaatatcta aatcttcccc
    46141 cgtaaggttg gcatacacaa tatcctgtcc ctgcatggcc tgcttcagtg ctgcatgatt
    46201 cagtacatcc cccataataa tctggctatt agttggatag ggtttgtgta tttttgctgg
    46261 ctgacgagca aataaagttt gtttaatggt ttgtttatct gccagttgat taatcacatg
    46321 gcgggctatc tgaccgccag caccaagaat gagtacattt ttcataatat ttctcatgaa
    46381 acttatcaca gatgggtgtt gaaaaaagga ataacttttt ccagcgcctc ctgaactgct
    46441 tccggtttgt catacagttc ataatgtgag aaatcgggta atgaaactaa ctgacgatcc
    46501 tgcgaaacca tcgcccggcc atagacttcc agtccatcac gataagcacc gaaggcaccg
    46561 actttttcac caactactac catcaccggc tgagtcagta acacttcagt aaatgcgaag
    46621 gcatcccagg ccagcgtttt ctgtgcatgt gagaacaaca tttttgtcgc acctcccggt
    46681 tgttgcccgc gtggtgtttt atagtagtca gttgcttcat aaacatcgcg ttcggtcagt
    46741 ccatgggctt ttgctgcatc caggctggcg ggaagtaatt cgttgatctg taattctcca
    46801 ccacgagcct ctttggttcg ttgcgacgcc attgcattca atgcgccaat cggatcgtaa
    46861 ttgctgaaac cttcccgaaa aagacgcccg atattaacac ccgtaatact cactacagct
    46921 ttaattcgtt tttctgtcag cgcggcatta atggcatagc cacccccccc cacacacacc
    46981 cagcacgcca atgcgttcag catcaacata tggcagagtt actgcataat cgataacacg
    47041 gctaatatcc tctacgcgct gtgtcggatc ttctatccaa cgaggctccc caccagattc
    47101 tccctggaaa ctggcgtcat aagcgagaac gacatagcct ttttcagcca atgccttgcc
    47161 atagatattg ccagacgtct gctccttaca actgccaaaa ggatggacgc tgataatcgc
    47221 cggatactgt tttgactcat caaaatttgg tggaaactga atattggcag caatatgcca
    47281 ggccatatca ctattgcgga tttgaatatg tttcattaca gagtttcctc aaagaacgga
    47341 gctaatactg aaattgcttc agccacatag gctttgccgt cataaagatc catatggttt
    47401 gcgccttcaa caatgtgata gcgtttatcc tggcttgatg cccgatcgta caggtcgtca
    47461 ctcatccatt tgctccctgc ctggctgccc gccacaatct gcgtcggctg agtcaggtac
    47521 acttccgcca tatggtaagc atcataggta ataatctggt taaggctgcg caaagtagcg
    47581 taacccggtg ctgttggata ctgcgcgcga ggggtgtggt aatactccca ggcctgacgc
    47641 agttcttcgt tcggcgcatc ggactccttc attggtgcca gtggcataat ggcgtattct
    47701 ccgctgctaa tatcgctggt tctggcgttt gaacccgctt caacgtatgg cagggcatca
    47761 atagatttca cattgttttc ccaaccatta cggaacatcg aaccaatatt gaccgcacta
    47821 acggtaccga tggccttgat gcggcgatcc tgaattgcag cattggctgt atatcctgca
    47881 ccggcacaaa ttcccatcgc accaattcgg gtattgtcga cataagaaag cgttgtcagg
    47941 taatcaatca cggcactgac gtcttcagta cgaatgtatg ggttttctaa ctgacgcggc
    48001 tcgccgccac tttcaccctg ataagatgcg tcataagcaa tagtgacaaa tcccttttcc
    48061 gccagttttt cggcataggt cccggccgtt tgttctttaa cgcccccacc tgggtgagat
    48121 accacaattg ccggatattg acgggtttca tcaaattttg gaggaaaata gatcactgca
    48181 gacaaagaga tggttggatt attgctgtta gtgaagctga ctttattatt catcattccg
    48241 ttcctcatgg agttgttggt tcgttttaac ggttggtgat atcactatag atattgatca
    48301 ttaagttgat tagaccctaa atcatgatta gacctatcat ttaaatgatt aatagattgt
    48361 atggagatac tgaatgaagc gtgaagagat cgctgatctg atggcgtttg tcgtcgttgc
    48421 agaggagcgt agcttcactc gcgcagcggc ccgcctgagc atggcgcagt cagctttaag
    48481 ccagatagtg cgccgtatag aagaacgatt gggattgcgg cttctgacgc gaaccacgcg
    48541 cagtgttgta cccactgaag cgggcgagca tcttttgtct gttcttggcc ctatgctgca
    48601 tgacatagat tctgccctgg catccctgag cgatctgcag aaccgcccat ccgggacaat
    48661 acgtattact actgtagaac atgcagcaaa aacgatattg ttaccagcca tgcgcacatt
    48721 cctgaaatca caccctgaaa ttgatattca gcttatcatt gattatggtt tgactgatgt
    48781 cgtttctgaa cgttttgatg caggcgtccg tctgggtggg gagatggata aagatatgat
    48841 cgccattcga atcgggccag atataccaat ggctattgtt ggctcaccgg attatttttc
    48901 tcgccgaagt gttccaacgt cagtgtcaca attaatagat catcaggcaa ttaatttgta
    48961 tcttcccaca tcgggtacag caaatcgctg gagattaata cgcggtggac gtgaagttcg
    49021 tgttcgcatg gaaggtcagc ttttactgaa tacgatagac ctgatcattg atgctgcaat
    49081 tgatgggcat ggattggcgt atctacctta tgatcaggtt gagcgggcga ttaaagaaaa
    49141 aaaactgata cgtgttttgg ataaattcac accagattta cccggttatc acctgtacta
    49201 tccacaccgt cgacatgctg gctcggcatt ctcattattt atagataggc tgaagtataa
    49261 aggtgctgtt tagcactact tgctgataca ttaatttaat tcttctctta acgtattctc
    49321 agttcctttc aacgttttgg ttatttttta ttcttcgtac aatggcgaca gatgctgatt
    49381 atgataccga aaactggttt gaacgtgtga agcccgaacg tagtgttcgg tgttctatgt
    49441 gctttgccgc attttggcgt c
//
LOCUS       DAZKFZ010000011        44881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000011 DAZKFZ010000000
VERSION     DAZKFZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(327..869)
                     /gene="ycfP"
                     /locus_tag="ACHOQW_000617"
     CDS             complement(327..869)
                     /gene="ycfP"
                     /locus_tag="ACHOQW_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP9571744.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(892..1917)
                     /gene="nagZ"
                     /locus_tag="ACHOQW_000618"
     CDS             complement(892..1917)
                     /gene="nagZ"
                     /locus_tag="ACHOQW_000618"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP9571745.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1928..2752)
                     /gene="thiK"
                     /locus_tag="ACHOQW_000619"
     CDS             complement(1928..2752)
                     /gene="thiK"
                     /locus_tag="ACHOQW_000619"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP9571746.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2733..3374)
                     /gene="lpoB"
                     /locus_tag="ACHOQW_000620"
     CDS             complement(2733..3374)
                     /gene="lpoB"
                     /locus_tag="ACHOQW_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP9571747.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3388..3765)
                     /locus_tag="ACHOQW_000621"
     CDS             complement(3388..3765)
                     /locus_tag="ACHOQW_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP9571748.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3768..4127)
                     /gene="hinT"
                     /locus_tag="ACHOQW_000622"
     CDS             complement(3768..4127)
                     /gene="hinT"
                     /locus_tag="ACHOQW_000622"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP9571749.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4467..6656
                     /gene="fhuE"
                     /locus_tag="ACHOQW_000623"
     CDS             4467..6656
                     /gene="fhuE"
                     /locus_tag="ACHOQW_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP9571750.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVNQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6716..8149)
                     /gene="ptsG"
                     /locus_tag="ACHOQW_000624"
     CDS             complement(6716..8149)
                     /gene="ptsG"
                     /locus_tag="ACHOQW_000624"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP9571751.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8444..9241)
                     /locus_tag="ACHOQW_000625"
     CDS             complement(8444..9241)
                     /locus_tag="ACHOQW_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9571752.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9252..10256)
                     /gene="holB"
                     /locus_tag="ACHOQW_000626"
     CDS             complement(9252..10256)
                     /gene="holB"
                     /locus_tag="ACHOQW_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9571753.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10253..10894)
                     /gene="tmk"
                     /locus_tag="ACHOQW_000627"
     CDS             complement(10253..10894)
                     /gene="tmk"
                     /locus_tag="ACHOQW_000627"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9571754.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(10884..11906)
                     /gene="yceG"
                     /locus_tag="ACHOQW_000628"
     CDS             complement(10884..11906)
                     /gene="yceG"
                     /locus_tag="ACHOQW_000628"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP9571755.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11909..12718)
                     /gene="pabC"
                     /locus_tag="ACHOQW_000629"
     CDS             complement(11909..12718)
                     /gene="pabC"
                     /locus_tag="ACHOQW_000629"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP9571756.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12838..14079)
                     /gene="fabF"
                     /locus_tag="ACHOQW_000630"
     CDS             complement(12838..14079)
                     /gene="fabF"
                     /locus_tag="ACHOQW_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9571757.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14167..14403)
                     /gene="acpP"
                     /locus_tag="ACHOQW_000631"
     CDS             complement(14167..14403)
                     /gene="acpP"
                     /locus_tag="ACHOQW_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9571758.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14614..15348)
                     /gene="fabG"
                     /locus_tag="ACHOQW_000632"
     CDS             complement(14614..15348)
                     /gene="fabG"
                     /locus_tag="ACHOQW_000632"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP9571759.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15361..16290)
                     /gene="fabD"
                     /locus_tag="ACHOQW_000633"
     CDS             complement(15361..16290)
                     /gene="fabD"
                     /locus_tag="ACHOQW_000633"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9571760.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16306..17259)
                     /gene="fabH"
                     /locus_tag="ACHOQW_000634"
     CDS             complement(16306..17259)
                     /gene="fabH"
                     /locus_tag="ACHOQW_000634"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9571761.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17327..18397)
                     /gene="plsX"
                     /locus_tag="ACHOQW_000635"
     CDS             complement(17327..18397)
                     /gene="plsX"
                     /locus_tag="ACHOQW_000635"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9571762.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18478..18651)
                     /gene="rpmF"
                     /locus_tag="ACHOQW_000636"
     CDS             complement(18478..18651)
                     /gene="rpmF"
                     /locus_tag="ACHOQW_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9571763.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18703..19224)
                     /gene="yceD"
                     /locus_tag="ACHOQW_000637"
     CDS             complement(18703..19224)
                     /gene="yceD"
                     /locus_tag="ACHOQW_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP9571764.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19423..20007
                     /gene="yceF"
                     /locus_tag="ACHOQW_000638"
     CDS             19423..20007
                     /gene="yceF"
                     /locus_tag="ACHOQW_000638"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP9571765.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20108..21067)
                     /gene="rluC"
                     /locus_tag="ACHOQW_000639"
     CDS             complement(20108..21067)
                     /gene="rluC"
                     /locus_tag="ACHOQW_000639"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP9571766.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            21640..24825
                     /gene="rne"
                     /locus_tag="ACHOQW_000640"
     CDS             21640..24825
                     /gene="rne"
                     /locus_tag="ACHOQW_000640"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP9571767.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25020..25973)
                     /gene="flgL"
                     /locus_tag="ACHOQW_000641"
     CDS             complement(25020..25973)
                     /gene="flgL"
                     /locus_tag="ACHOQW_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP9571768.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25985..27628)
                     /gene="flgK"
                     /locus_tag="ACHOQW_000642"
     CDS             complement(25985..27628)
                     /gene="flgK"
                     /locus_tag="ACHOQW_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP9571769.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27694..28635)
                     /gene="flgJ"
                     /locus_tag="ACHOQW_000643"
     CDS             complement(27694..28635)
                     /gene="flgJ"
                     /locus_tag="ACHOQW_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP9571770.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28635..29732)
                     /gene="flgI"
                     /locus_tag="ACHOQW_000644"
     CDS             complement(28635..29732)
                     /gene="flgI"
                     /locus_tag="ACHOQW_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP9571771.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29744..30442)
                     /gene="flgH"
                     /locus_tag="ACHOQW_000645"
     CDS             complement(29744..30442)
                     /gene="flgH"
                     /locus_tag="ACHOQW_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP9571772.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30495..31277)
                     /gene="flgG"
                     /locus_tag="ACHOQW_000646"
     CDS             complement(30495..31277)
                     /gene="flgG"
                     /locus_tag="ACHOQW_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP9571773.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31415..32170)
                     /gene="flgF"
                     /locus_tag="ACHOQW_000647"
     CDS             complement(31415..32170)
                     /gene="flgF"
                     /locus_tag="ACHOQW_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP9571774.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32190..33395)
                     /gene="flgE"
                     /locus_tag="ACHOQW_000648"
     CDS             complement(32190..33395)
                     /gene="flgE"
                     /locus_tag="ACHOQW_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP9571775.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33420..34115)
                     /gene="flgD"
                     /locus_tag="ACHOQW_000649"
     CDS             complement(33420..34115)
                     /gene="flgD"
                     /locus_tag="ACHOQW_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP9571776.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34127..34531)
                     /gene="flgC"
                     /locus_tag="ACHOQW_000650"
     CDS             complement(34127..34531)
                     /gene="flgC"
                     /locus_tag="ACHOQW_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP9571777.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34535..34951)
                     /gene="flgB"
                     /locus_tag="ACHOQW_000651"
     CDS             complement(34535..34951)
                     /gene="flgB"
                     /locus_tag="ACHOQW_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP9571778.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35106..35765
                     /gene="flgA"
                     /locus_tag="ACHOQW_000652"
     CDS             35106..35765
                     /gene="flgA"
                     /locus_tag="ACHOQW_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP9571779.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35841..36134
                     /gene="flgM"
                     /locus_tag="ACHOQW_000653"
     CDS             35841..36134
                     /gene="flgM"
                     /locus_tag="ACHOQW_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP9571780.1"
                     /translation="MSIDRTSPLKPVSTIQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36139..36555
                     /gene="flgN"
                     /locus_tag="ACHOQW_000654"
     CDS             36139..36555
                     /gene="flgN"
                     /locus_tag="ACHOQW_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP9571781.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36595..38130)
                     /gene="murJ"
                     /locus_tag="ACHOQW_000655"
     CDS             complement(36595..38130)
                     /gene="murJ"
                     /locus_tag="ACHOQW_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP9571782.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38240..39163)
                     /locus_tag="ACHOQW_000656"
     CDS             complement(38240..39163)
                     /locus_tag="ACHOQW_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9571783.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39165..39812)
                     /locus_tag="ACHOQW_000657"
     CDS             complement(39165..39812)
                     /locus_tag="ACHOQW_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP9571784.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39823..40407)
                     /gene="rimJ"
                     /locus_tag="ACHOQW_000658"
     CDS             complement(39823..40407)
                     /gene="rimJ"
                     /locus_tag="ACHOQW_000658"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9571785.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40643..41851
                     /gene="mdtH"
                     /locus_tag="ACHOQW_000659"
     CDS             40643..41851
                     /gene="mdtH"
                     /locus_tag="ACHOQW_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP9571786.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGDAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41915..42562
                     /gene="grxB"
                     /locus_tag="ACHOQW_000660"
     CDS             41915..42562
                     /gene="grxB"
                     /locus_tag="ACHOQW_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP9571787.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42696..43256
                     /locus_tag="ACHOQW_000661"
     CDS             42696..43256
                     /locus_tag="ACHOQW_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9571788.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43362..44408
                     /gene="pyrC"
                     /locus_tag="ACHOQW_000662"
     CDS             43362..44408
                     /gene="pyrC"
                     /locus_tag="ACHOQW_000662"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9571789.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44482..44727
                     /gene="dinI"
                     /locus_tag="ACHOQW_000663"
     CDS             44482..44727
                     /gene="dinI"
                     /locus_tag="ACHOQW_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP9571790.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10827 a        12127 c        11184 g        10743 t
ORIGIN      
        1 acagcaatga aaatgttctt cgctattgag aataacatga atggtgcatt ggtcatacca
       61 aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg ggtgagatca
      121 aaaaacagag tttattgttt attatttgag cgaatttgtg agagtgatat agcgtgaggg
      181 gtgtatgtag ggcgttcggt aggtctgata agaggcgcat caggcattga tgctcgactg
      241 tcggatgcgg cgtgaacgcc ttatccgact tacagaattc ggagcgcatc aggtaccggc
      301 aaggttacct gatgcgacga gggcatttac ccgagggttt tgaacgcttt aatgcgctgc
      361 aaatgcgggg agatattctt gaatttgtgc gtctgctctt cgtcccagac aatctcgtaa
      421 taatgatgca actcttcaga tgtccgctgg ctgttaagcg cttcatcatt acgcgacaaa
      481 atcaccaggc aacgatcgcg attcttctcg cggaagttgg tcacacactt agtggcaata
      541 tcggcatact cttccgggcg atcaatcttg ccttccatgt tctcataagg gaacaaatta
      601 gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa tccgttccgc ccagtatccg
      661 cccagaccaa cgccgcaaat caggggacgc tcgtcgacgt tcagttgcaa cattttgtcc
      721 acttctttga gcagatgctg catatcatgt ttcggatgcc gcgtactgta gcttatcaag
      781 cgtacatccg ggtcaataaa ctgcaattgt aagactttct cgtggttacc cggactgtta
      841 gagtcaaaac cgtgtaaata gataatcatc gcttcctcac ataagccagg gttagtgacc
      901 tgctttctct tcctgccagc gttcatgtaa ctgattcaga cgggcgctga tcgctttcca
      961 gcgagccgag tccatcagtt cctgtcgcga aaatgaacct ttatgataca aacgtgtaac
     1021 acgttctgcc ttgatcggtg acagattatc taacacgctg acggcccctt tacgattatt
     1081 gcagaccagg atcatatcgc aacccgcatc cagtgatacc tgcccgcgtt cggcataact
     1141 gcccataatc gcggcacctt ccatcgataa atcgtcagag aaaatcacgc cgtcaaaacc
     1201 cagttcctga cgcaaaacgg ttttcagcca gtaggaagaa ccgctcgccg gacgcggatc
     1261 aacatcactg tagatcacat gcgcaggcat aatggcgtcg agtttatttt cgcggattaa
     1321 cgtgctgaaa accgacatat ctttggcacg aatttccgct tgcgggcgtg ggtcgcacgg
     1381 cgtctctttg tgtgaatctg cagttactgc accgtgtcct gggaagtgtt tcccggtcgt
     1441 tttcattccg gcttcatgca tcccatcaat aaaccgactg gcgattgcca gggctttttc
     1501 tgggtcggca tgataagaac gctcgccaat cgccgcgctg atatgtccta catccagcac
     1561 tggcgcaaag ctgatatcaa tatccatagc gatcatttcg ctggccatca gccaacccgc
     1621 ctcttgcgcc agtttgccgc cctcttccat tcccaacagc gcagcaaagg attgtgctgc
     1681 cggtaagcgg gtaaaacctt cgcgaaaacg ctggacgcgt ccaccttcct gatctaccgc
     1741 caccaccaga tgattgcgcg atgctgcgcg gatctggcgc accagttcac gcaactgggc
     1801 aggatcatga tagtttcgcg taaagagaat cagccctccc accagcggat gcgccagtat
     1861 ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc aacattactg gacccacact
     1921 gctctcctta ttgttttatt aatagctgcc gccaggtgtc atcggccagc ctgataaatt
     1981 gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc tgctttgagc atcagcagcc
     2041 agggaaacca tcgcctgacc tgacgccata attgcgccgg ataaatcttc gcgcgagtgg
     2101 catagtcatt gaccaattgc cggtgctgtt cagtattttc cacccacacc gccgccagtt
     2161 ccagcgcgat atcaccatct cctgcatact cccagtcgat gagttttaac cctgacgcgc
     2221 tatgcactaa atttccggcg tggacatcca tatgcaatgg acttaagcgt aaaggccgtg
     2281 gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca acccactgtc cgccgcgccg
     2341 gatcgctttg ctgccagtac agttccagta acggcaacag cgttattcgc cagccaaaac
     2401 gtggttgttg atgtagataa tacagcaagc ctgccagttc gttggtatcc ggcaaatacg
     2461 tttttacctc gccgggcaga tagtcgacta ccatccagtc acggagatat aaatgcggct
     2521 tcggtgcaat gcatgcgggt agttgtgata aagcccgata ctggcgcaag aacgcggact
     2581 gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg ctggccctga tgttcaatga
     2641 gaaaactacc gccactaagc ccactattaa acgtcgtgac gggatgaaac tgcgggaaaa
     2701 agcgcgacag caattcgtcg cgcgtgatgg gattattgct gcgaaacggc acctttacct
     2761 gaccagataa tttcgcccgt ctgcaccagc atcagctgca tttgtagggt cggagcgtta
     2821 acgttgccag aggcgctgga gtacagcacg taatgagcgc cgacattgcg ggcaatgcct
     2881 atggctttac tacgggtgcc taaactatcc tgcggcgaca aacctaactg ttgcttagcc
     2941 atcgacagct gctgggcgga aaccagggta aatttcccgt tattagccag cgcatttcgc
     3001 agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag tacggttgtt aacgctatca
     3061 accagcagga cgctacccgc agtcaccccg tcagccccaa gcatcttact gaccatcggc
     3121 tgcattgcgc cattccagtc ataatggcga atatgcggcg caggcggtgc agtttgatct
     3181 tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac tgtggggaca
     3241 ggctgttgtg gctcggctgg ttgttccggc gctggtttca cttcttctac cggtgcaggt
     3301 tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa ggcgtagcga
     3361 ctcatttttg tcatcaagat tcacccctta caaatataga taaagtctga ctttgtgcgc
     3421 ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa tggtaacgct
     3481 gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt aaaaacgata
     3541 atgaacggta acgggttctt gcctttcgtt ataaagcgtt gaggatgctg aaggttgaat
     3601 atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata aagatgactc
     3661 catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac agcccaccag
     3721 cagtaacaac gccagagaca ccagcccaaa gcatcctttt ctcatcgtta cagaccttta
     3781 tgcgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat gtggtaaacc
     3841 tcttgtccgc catggcggtt ggtgttcatg atcagacgat agccatcttc ggcaatacct
     3901 tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg ctcatgctca
     3961 gctgagacgt cgttcacagt cggaatgagg atattcggaa tgatcagaat atgcgttggc
     4021 gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac gatatcggag
     4081 gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac attttccttt
     4141 ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta tagagtatgg
     4201 tcgagttaaa cctgctcttt caaccttaac tcacgatttc ttaagcaaaa aatcgttttg
     4261 ctgccgtata tatctccatt attcccattt ctgcctgcga atgcgctgaa aagacattct
     4321 ctgtttggtt tacacgacaa atatgaatgc gtatatttct catttgcatt tgcatttgca
     4381 agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga
     4441 cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
     4501 atcaagccat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
     4561 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
     4621 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
     4681 agatgactca acgtgatatt cctcagtcgg tcactattgt taaccagcag cggatggaag
     4741 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
     4801 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
     4861 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata
     4921 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
     4981 gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt gaatttaaag
     5041 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
     5101 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
     5161 actcatggct ggaccgctac aatagtgaaa agaccttctt ctccggcatt gtcgatgctg
     5221 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
     5281 gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
     5341 gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
     5401 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
     5461 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
     5521 tgctggttgg gccatacagt aattatggac ctggctttga ttatgtcggc ggcaccggtt
     5581 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
     5641 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
     5701 acttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg
     5761 gcaatttccc acaaaccgac tggtcaccac agagcctggc gcaggacgat accacacata
     5821 tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg
     5881 gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca
     5941 ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct
     6001 atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa
     6061 tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca
     6121 ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta
     6181 tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg
     6241 tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc
     6301 gctatattgc agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg
     6361 ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg
     6421 ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg
     6481 agcaaggtag ctacgcgttg gtggatctct tcacccgcta ccaggtgacg aaaaacttct
     6541 cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac gtggaaggtt
     6601 ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa
     6661 cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt
     6721 ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatcg
     6781 cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccagcct
     6841 gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt
     6901 tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac
     6961 ctgtcgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt
     7021 taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggga
     7081 acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaaa
     7141 acgacgtacc gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa
     7201 tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg atcggctcgg
     7261 tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc
     7321 ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt
     7381 tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa
     7441 tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag
     7501 gtacgttcca gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa
     7561 tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag
     7621 aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag
     7681 aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac
     7741 ggtagaaacg gttaaacatg tacgctgcga tcgcaccgga gataatccct ccgagtacgc
     7801 cagtatccgc caggtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg
     7861 caaccacggc catggtttta accatgatgc cataggcaac aactgcagcc agcgcggata
     7921 cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg
     7981 caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga
     8041 aattcgcgga accgacgccc agcagaatac ctgcgatagg cagtacggat accggcagca
     8101 tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg
     8161 ctcctgagta tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc
     8221 gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa
     8281 tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca cattttgaca
     8341 aaacatatgt cacaatattt caaacgtggt tattcaccgc ccaattttcg gcggtgaact
     8401 ttactcgttt tagtcattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa
     8461 ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt
     8521 cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag
     8581 gttgattctc ttttcctcga tgcggcaccg gcgcaaggta aggtgagtca gtttccacca
     8641 gtaaccgatc cagggggaca taacgcgcag catcgcgcag ttgctccgca ttacggaagg
     8701 tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttgccc gccgtttctc
     8761 tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa
     8821 ttgccagcgt atcggcgcgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac
     8881 gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt
     8941 aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc
     9001 gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata
     9061 cgacgttgtc acgttcgcct accagatccc gcatgtgtaa ataacccggt aatgttgtgg
     9121 cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt
     9181 ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca
     9241 tgatgtctct cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca
     9301 atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac
     9361 tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg
     9421 tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga
     9481 tggcgtttta gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt
     9541 tcatgattaa gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac
     9601 gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg
     9661 ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact
     9721 tcgcgtgaaa gccaggtcac ggcgtactgt tccggcggcg gcgcaaggta atgtaaccga
     9781 caacgactac gtaacgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag
     9841 gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt
     9901 aaggcagcat cggttaccca gacaactttc gcaccaccta agcgtgcgtg ctcattcagc
     9961 ttttcggtga cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc
    10021 agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg
    10081 caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa
    10141 gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc
    10201 tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt
    10261 ccaactcctt cacccagcgg gtcacggtag tgcggattgc atccatcacg gcctccagcg
    10321 gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg
    10381 cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg
    10441 cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt aagtcggggc
    10501 gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc
    10561 ctccgccctg atacgcctga gtggagagat cgtggcgatc gccaataacc caggtgccgt
    10621 tagccagcgc aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca
    10681 gaacttcggc tttatcggta atgatttcat cgcctaccga tttgatatcc agcaccaggc
    10741 ttcttaactt ttcggcaagt tgcgtaccac caggttcccg agtgaaaacc atgtcgcgga
    10801 tacccagttg ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt
    10861 ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa
    10921 tcctgcacag acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca
    10981 tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc
    11041 gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct
    11101 gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa
    11161 atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag
    11221 gcaacctgat cgcgttcact ggcaatggcg gtttcttttt cgataattga tgccatcgtc
    11281 accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg
    11341 accgctttca ccattttctt gtgcgctcgc ttgagtaacg cgacatcggt ggtattggcg
    11401 gtatacatcc aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca
    11461 agtgcctgcg ctacggtgat gtacttatca tcgctcagcg tgtgcttgat atacggggcc
    11521 tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagagggaac
    11581 tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta
    11641 aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag caaccattga
    11701 aaaacccgtg ggcgattgat gatcttatcg gcataaagct gttcaccaag cgccagacgt
    11761 ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca
    11821 agatagcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac
    11881 aagattatca ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat
    11941 attcatataa cgttgctgac gaaaaggaga catcgccaca ggcacgtacg ggcatcactg
    12001 gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcttcc agagaggctt
    12061 gcacttcgac aagctgataa gaggattgtg ccagcaaacg gatacagaat tgtcgcataa
    12121 tgccgttaac acctgcctga tccagtcgcg gcgtataaac tacgttgccc ttccgccaga
    12181 acaaattagc tgcacagcat tccgtaaccc ccccttcgct gtcaaggacc agcgcctcat
    12241 cagcgtttgt ctgctcaaga tgagagcgga tcaatacttg ctcaagccga ttaagatgtt
    12301 taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtgatcc
    12361 cttcgttacg caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg
    12421 ccggtccact gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt
    12481 tcagtacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc
    12541 agaagtcaca ggaaatcatc aaccgctgac aagcatcctg tagtcgctgg atatgcgccg
    12601 ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact
    12661 gcgttgcccg atcgcttact gacagcgatt cctgcttata accgttaatt aagaacatac
    12721 cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat
    12781 aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa tgacaactta
    12841 gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt
    12901 gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc
    12961 atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga
    13021 gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga
    13081 gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc
    13141 tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc
    13201 aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg
    13261 cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat
    13321 tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc
    13381 gcccagtacg aaaccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc
    13441 attgcgggta gataatgcac gtgccgcgcc aaaaccacca acacccagcg gcgtactggc
    13501 tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc
    13561 atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg
    13621 caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa
    13681 gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag
    13741 tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt
    13801 tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa
    13861 ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac
    13921 taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc
    13981 actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa
    14041 catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc agggagggaa
    14101 aaaatgattc tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat
    14161 gttcacttac gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt
    14221 ctcagcttct tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc
    14281 aacggtgtca agagaatccg cgcctaggtc ttcaacgaaa gaagcattgt tggtaacttc
    14341 ttcctgctta acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct
    14401 catactctta aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat
    14461 gttgaaaaat ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg
    14521 ccttaccccc caataacgca aatcattttg cactaattat tactcataac cacgcagact
    14581 gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac
    14641 atgcaaagtt tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt
    14701 ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc tgcaccagga tacccgcacg
    14761 ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt
    14821 tacagtaata ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc
    14881 cgctttcgcc gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc
    14941 gatagtgata atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag
    15001 acggaaaaca gatgaaaggt tggtttcgat aatatcgttc cactcttcat ctttcattcg
    15061 cattaacagg ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa
    15121 ttctgcgcga attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat
    15181 cagacctttg ccgttagcac ctaaataatc actgatcgcc tgagcgccat tttcactggt
    15241 cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat
    15301 tccgcggctt gcaccggtta ccagtgcgat ttttccttca aaattcatga tgttcctctt
    15361 ttaaagctcg agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt
    15421 gtcgacaatg cgtttcgtca ggccagtaag cactttgccc gggccgactt catagagatg
    15481 ttctacgcct tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa
    15541 ctgacgtacc agtgcgtcac gaatggcatc accattggtt tcgcatttca catcaacgtt
    15601 attcacaaca ggaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt
    15661 gtcggctgct ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagagc
    15721 gcgttttgcg cccgcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc
    15781 ggcaataacc acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc
    15841 agcttcttca cacgcttttc caatagacgc atcatccaga ccgatgattg ccgccatagc
    15901 gcccgttcct tccggtacgg cttcttgcat gaacttgccg cgcatctcaa ccagacgcac
    15961 cgcatcagcg aaatcaatca caccagcgca aaccagcgcg gagtattccc ccaggctgtg
    16021 accggccatc attgccggtg ctttaccgcc atgctgctgc catacgcggt acagcgcaac
    16081 agaggcagtc aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc
    16141 ctgctgggtc agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc
    16201 ttcgacaatt ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg
    16261 tccagggaac acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag
    16321 cgcggagccc caggtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt
    16381 gatgcgcccg tcgcgtacag cttcatccag cgcgcacggg acagaggccg cagaggtatt
    16441 accgtggcga tccagcgtca ccacgacatt gtccatagac ataccgagtt ttttcgccgt
    16501 tgcactgata atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc
    16561 aagattattc gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt
    16621 gaagacttcg ttgcccgcca tcgtcagatg aattgaattc tctggattca cgcggtcggc
    16681 gtttggcagc gtcagcaatt caccataact accgtcggca tgcagatggg tggaaatgat
    16741 tcccggctct tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat
    16801 agtcccacga tctgttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc
    16861 atacttcacc gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc
    16921 tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta atgcccaaca tgctttgaat
    16981 ctgacaagct gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc
    17041 aatctggtct ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat
    17101 ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac
    17161 aatccactcg tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc
    17221 gggcagatag ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg
    17281 ttatataccg tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc
    17341 cgcttttgcc accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg
    17401 caattcgctg aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa
    17461 aagctcgctg attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac
    17521 aggcgccgtt atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta
    17581 gccaacgctt taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca
    17641 gcagagaaag gaacatcctg acaacacctt ccatcgtctt taatgtgaca tttcctgtaa
    17701 agccgtcaca aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc
    17761 cgatataatt gatagaaggg attgttttaa gcaccgctga ggcatcccga atactatcga
    17821 gaccctttac ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca
    17881 ccacctcttc agccaggact gagcccataa tggcaaattg caccaacatt gtgctgtcac
    17941 aatcgacgtt ggcccctaag tcaaggacca ccgttttgcc cttttgctga tgtggtaata
    18001 ccgtcaccag cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc
    18061 ccatcagtgc cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca
    18121 gctccagggc cacgcgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg
    18181 catcactggc gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt
    18241 cagctttagc aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt
    18301 gcgaattaga attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc
    18361 ctcccatgac atctaacgcc agggttagac gtgtcaaggt atcgtcgccg cccggtttgg
    18421 tctttcccca gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta
    18481 cttagcgatg accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg
    18541 tttttcacca gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga
    18601 acgacgcatg ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac
    18661 tcctcaatta cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct
    18721 ggctaatacg gcaaatgggt ttggcttttg cgcttcttca ggcagttcac caaagaccat
    18781 gtccgcttcg gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag
    18841 gatgatttca tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat
    18901 cggttcatac gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca
    18961 atacgttgtg tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac
    19021 cgtcaccttc gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat
    19081 ggagcattcc acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc
    19141 aggagtatag ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt
    19201 caggggtaat tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc
    19261 aaagaaaaag gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact
    19321 gctttatttt cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt
    19381 aaaatggcta tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta
    19441 gcctccacct cgccctggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt
    19501 gcagcacccg aggtcgacga aactccacac agcgacgaat caccgcgaca gttggtgctt
    19561 cgactggcac aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt
    19621 ggttcagacc aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa
    19681 aatgcccgtc tgcaattacg caaagccagc ggcaatatcg tcactttcta tactggactg
    19741 gcgttgttta attcggcgaa tgggcatctg caaacagaag tggagccttt tgacgtccat
    19801 ttccgtcacc tgagcgaggc ggagattgat aattacgtgc gtaaagagca tcccctgcac
    19861 tgcgcgggta gctttaagag tgaaggattt ggcattactc tgtttgagcg tttagagggg
    19921 cgtgatccta acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg
    19981 gaagggaaaa acccgctgat gggatagttt tttgcgccgg atgcggttac gtcgcatccg
    20041 gcaatcggtg ctggcggcct gatgcggcgt aaacgcctta tcccattatc atgcttatat
    20101 gctcgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacca tcatccatcg
    20161 gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt
    20221 gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt
    20281 caccgtagcg atcgtcaaag gcaatcggat gacctgcata ctgtgtatgc acacggatct
    20341 gatgagtgcg ccctgttacc ggactacaac gcaccagggt ggcaaatgca tagcgttctt
    20401 ccactttaaa gcgtgtttcc gacggtttgc cttcctgact cacacgcacg atacgttcgc
    20461 cgctttgcag aatatttttc aataacggcg cctgaacgct cttcacatgc gactgccact
    20521 gaccgcgcac cagcgccagg taatcttttt gcatcccttt ttcacgtaat tgctcatgca
    20581 gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaggtg tcccggtcaa
    20641 gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcaataa
    20701 cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca
    20761 ggatgtgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg
    20821 gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tggaatacgc acctcatcac
    20881 ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact tcgcctttac
    20941 gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttgt
    21001 cgatacgttg ccccgcttcg tcggcggtga tagcaacaat ttttacggat ggagtctctg
    21061 ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct
    21121 tatatttagt tttgcacctt attacttcac tacgtgatca ctttattgat ggttattaaa
    21181 ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca
    21241 tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta
    21301 aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc
    21361 gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag
    21421 atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc
    21481 cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt
    21541 tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg
    21601 cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac
    21661 gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg
    21721 gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc
    21781 cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc
    21841 ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt
    21901 cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa
    21961 gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg
    22021 gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc gccgtatcga aggcgacgac
    22081 cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc
    22141 gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc aatgggattt aagcttccgt
    22201 ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggcccc gttcctgatt
    22261 catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt acttacgtca ggacatcggc
    22321 gaaatcctta tcgataaccc gaaagtgctc gaactggcac gtcagcatat cgctgcatta
    22381 ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc
    22441 cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg tctgccatcc
    22501 ggtggttcga ttgttatcga cagcaccgaa gcgttaacgg ccatcgacat caactccgca
    22561 cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta acactaacct cgaagctgcc
    22621 gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc
    22681 atcgacatga cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg
    22741 cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg
    22801 tcccgccagc gcctgagccc atcactgggt gaatccagtc atcacgtttg tccgcgttgt
    22861 tctggtactg gcaccgtgcg tgacaacgaa tcgctgtcgc tctctattct gcgtctgatc
    22921 gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatc
    22981 gcttcttacc tgctgaatga aaaacgttct gcggtaaatg ccattgaaac tcgtcaggac
    23041 ggtgtgcgct gtgtaattgt gccaaacgat cagatggaaa ccccgcacta ccacgtgctg
    23101 cgcgtgcgta aaggggaaga aaccccaacc ttaagctaca tgctgccgaa gctgcatgaa
    23161 gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc ggaacaacct
    23221 gcgctggcaa cctttgccat gccggatgtg ccgccagcgc caacgccagc tgaacctgcc
    23281 gcgcctgttg tagccccagc accgaaagct gcaccggcaa caccagcaac tcctgcacaa
    23341 cctgggctgt tgagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa
    23401 accaaaccga ccgagcaacc agcaccgaaa gcagaagcga aaccggaacg tcaacaggat
    23461 cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg agcgccgcga cacccgtagt
    23521 gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaagcacag
    23581 cagcagactg ccgagacgcg tgagggccgt cagcaggctg aggtaacgga aaaagcgcgt
    23641 accgccgacg agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa
    23701 cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc
    23761 gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag
    23821 aaagtgcgtt acgagcaaag cgtagccgaa gaaacggtag tcgcaccggt agctgaagaa
    23881 actgtcgctg ccgaaccaat tgttcaggaa gcgccagctc cacgcacaga actggtgaaa
    23941 gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat
    24001 aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg
    24061 agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccaatc gccaatgccg
    24121 ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatccg
    24181 attgtacgtc cacaagatgt acaggttgaa gagcagcgcg aacaggaaga agtgcaagtg
    24241 cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg aaccggttgt tagcgcgcca
    24301 gttgttgaag aaatggctga agtcgtagaa gcccccgtac cggttgccga accgcaaccg
    24361 gaagtggttg aaacgacgca tcctgaagtg attgccgccg cggtaactga acagccgcag
    24421 gtgattaccg agtctgatgt tgccgtagcc caggaagttg cagaacacgc agaaccggtg
    24481 gttgaaccgc aggaagagac ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg
    24541 gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcgcagca
    24601 gaagttgaaa cggtaaccgc ggttaaacca gagatcaccg ttgagcataa ccacgctacc
    24661 gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac
    24721 tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaacg
    24781 catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaagta
    24841 atcaagccct ggtaactgcc agggcttttt tatttcatct ttgaatcaac atattaaaat
    24901 tcatcgaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaat gcccgccccg
    24961 gcgatccctg agcgggcaga caaaacatac ccagtttcat gatatgtttt aaagcgagct
    25021 tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt atacgatgcc
    25081 tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc cacatcaacc
    25141 agatcgctca tctgctgcgt ttgccctaat gcgcgatcgc tacctaatga atccagcgac
    25201 tccagttcgt tcagctgcgt gcctaattcc gcgcgaacgg tcaggacatt gttcagcgag
    25261 tttttcagtc cgcggttggt tttatctaac gccgcagcgg cggtttcttt atccgcttcg
    25321 ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc aaaaagattg
    25381 gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact gtcgaaaatt
    25441 ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg cttaatactt
    25501 tctgttccgc cgacataatc accgttaacc tcactaaacg gcgcagtctc tgttttataa
    25561 ccggcaaaaa tgtagcgccc gttaccgtca gtggtgttcg ccagattcag caactggtca
    25621 cgaagcccct gaatatccgt agccagcgag gcccggtcat tgtcactcaa ggtgccattg
    25681 ctggcgtaga caattttttc ctgagcattc tggatagcgg tggtgacctg gctaagtaca
    25741 ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt
    25801 tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg
    25861 ttaacgactc gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa
    25921 ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct
    25981 ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg
    26041 cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga
    26101 caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc
    26161 tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt
    26221 cgttaaagga tttcgctccg cccaccgttt tactgttgct ttgcagatcc agcagggcct
    26281 gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgct attttcgctt
    26341 cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc
    26401 tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac
    26461 cgttggcatc tggcgtcacc gtaaaagtgg tattgctggc aaggcgggtg acctgccact
    26521 gattattatc gaacgagatt ttgtaatctg tcgccagtac cgcggaggca tcagttaccg
    26581 tggcaccgat cgcaacgtta ccgttgtttt tagtgttttg cagaaccgcg ggcttaccga
    26641 tagcaaagaa atcttcaccc tcatcgccgt tggcatcaaa tccggctttg tgttggctgt
    26701 tgaaagcctc agcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat
    26761 cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga
    26821 tctcaatatt gcctgccgtc ccatcaacat aagcgacagt cgtacgagaa gggtcggcgc
    26881 tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg
    26941 ccatcgtgat gttataagtg ccgccatcct gaacgctgac ttctacacca acaatctggt
    27001 ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc
    27061 ccacgcctgt caggcgggag atttgatcgt tcaggctggc gatttgttta gcgtaattgt
    27121 tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgaagat
    27181 actgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc
    27241 gcgctgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca
    27301 tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct
    27361 catagcgggc agtcaggccg ctactttgcg tctgcgccgc acgtaactgg ttggtaataa
    27421 acgcatcata ctcacgctgc acaccagaaa cgtagacacc attaccaacc cagccgccag
    27481 cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt
    27541 tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc
    27601 tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca attacagagt
    27661 attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct
    27721 gtaggttttg ctcaccttgt cgcttatcga tttcatctgc tgaatcatgt tggtgagttt
    27781 acgggcatag tgaggatcgg tggcatagcc cgcgtcctgt agggcctgcg ccccctgctc
    27841 cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta
    27901 atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc
    27961 ttcgccattt tcatattcag tcgtggtgat ttcagtgact ggccctttcc agttgccaga
    28021 ggctttgaca ccaaacaggt tatagctcgg ctcgccgttt tcgcggcgga tttgccgttg
    28081 cccccagcca gattccagcg ccgcctgagc gaggatcaaa tgatgtggca caccgctttg
    28141 ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctttac tgtcacctgg
    28201 cagcgaatca tcgtagttac gtggcacggc cttttgcacc agctgcgaaa gcgcctgatt
    28261 ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc
    28321 tggcaatggt tgttctggcg tcatctgctt aaccatcatc tctgcaagcc ccagaccttt
    28381 gcccgtcgtc atctgttggg caatctgctg gtcatacata ctggtataca ggcgagtgtg
    28441 ctcgctgctg aacaggccat cttttggtaa cgcgtcgcgc atgcttttca acatcatctg
    28501 cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct
    28561 cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt
    28621 gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt
    28681 gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag cgcgcgcacc
    28741 acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaag
    28801 tcgatctgcg tttgtggcgt taccacggtc tgtccgccac caaacggtgt atctggttgg
    28861 ctgacattgg cctgacgatt gactgttact gagagatttc cctgcgctat cgcgcagctg
    28921 tcgagagtca cttcgcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta
    28981 gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg
    29041 gaactgttgc cactcggtac gcgcacctga atagtccgcg catccaacgc ggtggcgctg
    29101 ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg
    29161 tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggtaa ttcacgttca
    29221 ataactgcac cattggtgat ccgtccaccg ttcagttggt tcacctgaac actgctaccg
    29281 ccagcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga
    29341 ctgtcaacgc ccttaagcgg agtcatcaac aatgtgccgc cacgcaggct tttggcattt
    29401 cccatggaag aaaccaccac gtcaatggtt tgcccctgac gtccaaacgg tggaagtgac
    29461 gccgtcacca ttaccgcagc aacgtttttt agctgcatat tggtgcccgt cggaacggta
    29521 attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc
    29581 tggtcaccgg tgccatccag ccccaccacc agaccatagc caatcagtga gttttgcctt
    29641 accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt
    29701 agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt
    29761 taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta
    29821 cgtattcaat gcgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag
    29881 tgcgtggatt aaccacgcca gagaagcgaa taaattcggt accctgatta atggcaatct
    29941 gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca
    30001 acgtgccgct aaaggtatta ctggcattgg ccccgccttt tccgttgaac gtgttaccac
    30061 cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag
    30121 tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg
    30181 cgctaacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat
    30241 cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt
    30301 tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg
    30361 tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag
    30421 tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcaataaacc ggtgaacctg
    30481 ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc
    30541 gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc
    30601 acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc
    30661 ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg
    30721 ctctccagcc cggtgtcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga
    30781 gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac
    30841 gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc
    30901 ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgagcc atctggcaac
    30961 atcacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttgtt ggtctgcgac
    31021 aggttgcctt ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt
    31081 aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga
    31141 taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc
    31201 aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc
    31261 cataatgaac tgatcataaa atgtcctgta gtggattgct gacaagcaga gcagaagcac
    31321 gcggcggtgc gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaacagtcc
    31381 gctcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg
    31441 cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg
    31501 cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc
    31561 ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg tccacgcgtg gcctgggttt
    31621 ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca
    31681 ccagtttcag acgccccact ggcgcaaccg tatttgccgg atcgcccgga ttcagcgccg
    31741 agattgttcc atcggcagct atcgtgattt ccgccccttc cggcacagca attggccccg
    31801 cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc
    31861 tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccaac cagccatcct
    31921 gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat cttgccgggc gttatgtctg
    31981 cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct tccaccggca
    32041 ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt agaggcattg gccagattac
    32101 tggcggttac cgcctgttga ttcagcgtct ggcttgctgc tcccatcgcg gtataaattg
    32161 cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt gttgaggatc
    32221 tggtcctggg ttttgatggt ctgggcgtta gactgatagt tacgctgggc aacgatcata
    32281 ttgaccagtt ctttactgag atcgacgttg gacgcttcca acgcaccgtt ggtcagggtg
    32341 ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc cagaagattg cgtcgcagac
    32401 cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc cagtacaatc
    32461 tgccccagca gttgggtttg ttcgttggaa tagttgccga caaccgtacc gtcatcattg
    32521 atttgataac tcaccagatc gcccggtttg tagccattct gggtggttgc cacaatgttg
    32581 ttagcgccgg tattttgctg catggagttg aggaagctca ggctaaacgt ggcgggttct
    32641 gcgccgttaa ttgcgccggt ggtaatattc tctgttggat ttgaggtcag aacgccattg
    32701 gcattaaaca ccagcttcat tgcaggctcg gctgtacctg ttggatcact gctatcctgg
    32761 gtgtagacct gccagttatt atccccggtc ttcacaaagt agacgctcat gtcatgagca
    32821 ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg
    32881 gcatcaaatg cgttaacaga gggaagcgga tcgctggaat tcaggttgat ctgcatcgac
    32941 gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatgtt agtcggattc
    33001 gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct
    33061 tgcatattca ccaggttacg attttcatcc agcttaaatt gtccgttacg gctgtagaac
    33121 accgaaccgt tgctatctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga
    33181 ccacgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt
    33241 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    33301 aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    33361 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    33421 taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta
    33481 ttaccgttgt tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc
    33541 agttgtgtac caccgttact ggcgctaatc gctacgttgt aagaaccgtt cggcgcagtg
    33601 ctgccgtcag tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca
    33661 atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct
    33721 gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    33781 ccggttcccg caagaacagt ggtgccgggg atcatcacgc cgtgaccgat caggttactg
    33841 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    33901 ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc
    33961 attggattgg tcgggtcctg gtttttcagc tgcgccacca acaaagtcag aaaactgctt
    34021 tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta
    34081 tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg
    34141 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc
    34201 tgcgtgacgc cgacatggtg ttaaccatct ctccgacaac atcaacgttc ggcattttta
    34261 cgtagccctt tgcatccgcc agcggattac ccggttcata aaccagtttg tccggggcct
    34321 gactttctat aacatcggca acctttacgc cgcctgtcgc agcacctggt gcagcgttaa
    34381 cctggaatac cacctgtttt gcccgatatg gctgtccatc aggaccggtc acgctatcag
    34441 cattcgccag attactggcc gccacgttca ggcgctggga ctgggcagtt aacgccgacc
    34501 cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca
    34561 tcatgccttt gatttgcccg ctcaacgcgc taaggctcat ctggtattgc aggctgttat
    34621 cggcaaactg ggtgcgttcg cgatccatat cgacggtatt accgtcaagc gaaggctggt
    34681 ccggaatacg gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc ggaatgtgtt
    34741 gcgttgaggt catcgtcaga gcaaccacac tggttgcatt ccgtccacgt tgcatgactt
    34801 ttttaagttc actggcaaaa tcgatatcgc gcgcctgata accaggggta tcggcattgg
    34861 cgatgtttgc tgccagcact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa
    34921 aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattctgcc
    34981 atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta
    35041 tttatgccgt tgatggtcat tgtggacagg tacaattcac gttgtagaaa tggctggggg
    35101 cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa
    35161 gtgcggcgag caatctcaca tcgcaattgc aaaccttttt tagctcccag ctcgcaggga
    35221 taagtgatga ggttcgtgtt tctattcgta cagcgcccaa tctactaccg ccgtgcgagc
    35281 agccattgct ttcgatgagc aataattccc gcttgtgggg caatgtgaat gtgttggcac
    35341 gctgcggtaa cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg
    35401 ttgccgcgat gcccattgtg cgaggaggaa agctgggagc tggcaatgtc aaactgaaac
    35461 gcggacggct ggataccttg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg
    35521 ccgttagcct gcgcgatcta tcacccgatc aacctatcca gttaacccag tttcgccagg
    35581 catggcgggt aaaagcggga caacgcgtca acgtgatcgc cagcggtgat gggtttagcg
    35641 ccaacgcaga aggtcaggcg ctaaacaatg cagccgtcgc acagaatgcg cgggtgcgca
    35701 tggtatctgg acaggtagtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc
    35761 tgtaagctgt taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg
    35821 ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag cccgtaagca
    35881 ccattcaacc gcgcgaaacc actgacgcgc cggtaacgaa cacccgagcg gcaaaaacaa
    35941 cagcctccac cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg
    36001 gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg
    36061 aactaaaaat ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact
    36121 tgcagagtaa ttaatcgtat gacgcgtctt gcagagatcc tcgaccagat gtccgctgtg
    36181 cttaacgatc tcaaaacggt aatggatcaa gagcagcaac atctctctat ggggcagatc
    36241 aacggcagcc agttgcaatg gattacagaa caaaaaagct cactgctggc aacgttggat
    36301 tacctcgaac agttacgcag gaaagaaccc aatacagcaa atagcgttga tattagtcaa
    36361 cgctggcagg aaattgctgt gaaaacgcag caactacgcc aaatgaatca acataacggc
    36421 tggttactgg aaggacagat tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat
    36481 caggaaccga cgctatatgg ggcgaacggt cagacctcaa caacccatcg cggcagtaaa
    36541 aagatttcga tctgaagatt atctccggcc tgcactgcag gccggaatgc attgttacac
    36601 cgtccggcgg gcaaattctt taactttgaa gcccagtacc gccagtgcag cgaagtacgc
    36661 ggcaatcccc gccagcacga ccgccattaa acgcagcaaa cgccagggca tggtacccaa
    36721 tgaccactcc ggcatgatat gtaacatacc taaaagcacg ccagacatca ccagtaccgc
    36781 caccaccaga cgcaacagaa acgccatcca gccaggttgc ggggtaaaga ttttctgctt
    36841 acgcaactgc cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga
    36901 cagcccggca tgtttcaacg gaccgataaa cgccaggttc atcaattgcg ttaaaatcag
    36961 cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg cgggaataaa agccaggagc
    37021 caacactttc actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg
    37081 ggtcatcagc gcatcaaagg cggtaaattt accgtactgg aacagcgaaa cggttaacgg
    37141 accggaaaga atgcccaacg caaccgcact cggcaacgcc aacaggaaac aaagacgcag
    37201 tccccagtcc atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag
    37261 cgacggtagc aaaatggtgc caagcgccac gcccagcaca ccggacggaa actccattaa
    37321 gcggtcggcg taatacatcc atgacaccga accggaagca agaaacgagg caaaaatggt
    37381 gttgatgatt aaggagatct ggctcacaga gacgccaagg attgccggtc ccatctgttt
    37441 caccacacgc attgctccgg catcgtggaa gttaatgcgt ggcaaaacca gcatgccgat
    37501 cttcttcaga tgcggtagct gatacaccag ctgcaggacg ccgccgaccg tcacagccca
    37561 cgccagcgcc agcaccggtg ggttaaagta cggtgcggca aacagcgcga aaccaatcat
    37621 gctgatgtta agcagtgttg gagcaaacgc cggaatcgag aagcggttcc acgtattcag
    37681 aatcgctccc accagcgacg ccagggagat cagcaagata tagggaaagg taatctttag
    37741 tagctggctg gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat
    37801 gatcacccac ggtgcggcga gcatgccagc gaccgtcaca accgccagcg caagtgtcag
    37861 caggccagaa acataagaga caaagacccg cgtggcgtct tcaccctgct tacttttata
    37921 ttccgccaga atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa
    37981 caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat
    38041 tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct
    38101 gacggcggcc agcgatttta ataaattcat cggtgttcta atccagaccc acgggcaaaa
    38161 cgcctgcaag gcaggcgctg aaagaggcga atagtctacc tggattatgg tgaattgcta
    38221 ccgccagatg ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac
    38281 gttgcgccag cactgcctgt tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt
    38341 caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc aagcgtgctc tgccaaccag
    38401 gaatcggttt attcactaca ccctgcccac gctcctcacg ccattcgcgc atttccgtaa
    38461 tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc acgctgactt ccggcacggc
    38521 gatgcataca agtggtgatc tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca
    38581 tttcgccagc gtcgttagtc agtagcgtac cgccatccag agaggctttg ccgcccgaca
    38641 accacagcgc ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt
    38701 gtggcccgac gctattgcta cgatgtttat ccattcttag cgaggctgcg gtggcgagtt
    38761 gcgtttttaa ctcacagtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca
    38821 gttttttccg cgccgccagt tccaccagcc gttcagcatc gcgcagattt tctgccagcg
    38881 gtttatcgac acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtgac
    38941 tggcggtgct ggaatgtaca aaaaccgcat cgcaactggc ggcaaggctg gataacgaat
    39001 cggcataagg aatgcgccag ctttcacaaa ttggcagggc tttcgcgcgc gtaggcgacc
    39061 aggctccttg taacgtccag tcagacgctg ccgccagcac cggtaaccac gctttttgcg
    39121 caatgccgcc taatcccact acgccgatac gtaatttttt cactttaatc tcccaggtgg
    39181 gccagcaacg aatcaagacg ctgtttcagt tctgccactt cgatttccag ggcttcgacg
    39241 cgggcctgta aatcaccgtc aaccgcgttc gacatatccg ttaccgccgg ctgatcctct
    39301 acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga
    39361 cgcaccacaa aaggaccatc ttcgcgattc gccagttgtt ccagcgtcaa ctccacttcc
    39421 gccatatcgc tgaattcata cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg
    39481 gcaccacgca ataacaacgt ggtgattaac gccacttctg cggcgctcag tttcagatcg
    39541 ccaaattctg aattacaaaa acgttgctca tatttggtga cccgattacc aaaaccgctc
    39601 actgtgcgta gataatgacg tttgaccaga ttatccagct gttcctgcac ttcagattca
    39661 ctcagattca tgaccggttc acggttcgtt ttctgattac aggccgtgac tacaccattg
    39721 actgagagcg ggtattgctc cggcgtggtc acctgctttt ccagcaagca accaatcaca
    39781 cgagcttcca gggcagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca
    39841 gtctggggta gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata
    39901 gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt
    39961 attgtgcggc atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg
    40021 aatggctgcg gtcagggctt caaacatgag tcctttgccc tgccattttt gcccaatcga
    40081 ataaccgaga tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc
    40141 aataatctct ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac cttgtttatg
    40201 aaattcgtta atcatcccca gcctggcctg ccagcctgat ggataacagt ggctttcgtc
    40261 gcgcactggc tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgccagacg
    40321 ccaggcatca cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt
    40381 tggcacgtta ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat
    40441 acgctgtaag tattactata cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt
    40501 ttctgcatta ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa
    40561 atattgtccc cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat
    40621 cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag gcgaggaacc tgggtaaata
    40681 tttcctgctc atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat
    40741 ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc atggtcggta ttgccctcgg
    40801 cctacgccag tttattcagc aaggtctggg tattttcggc ggcgcaattg ccgaccgctt
    40861 tggtgccaaa ccgatgattg ttaccggtat gctgatgcgt gccgccggat tcgccacaat
    40921 gggtatcgcc cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg gactcggtgg
    40981 cacgttgttt gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc cacaacagcg
    41041 tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca ttggcgcatt
    41101 gttggggagc tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggccgtttt
    41161 gtttgtgctg tgtgcagcgt tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt
    41221 acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc gacaagcgtt ttgtcaccta
    41281 tgtcctgacg ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat
    41341 tatggtcaac gatgtggctg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc
    41401 gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc attttcgtct
    41461 ggaacaccgg ttgatggctg ggctgttgat aatgtcatta agcatgatgc cggtgggcat
    41521 ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg ttttatatcg ggtcgatcat
    41581 tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag
    41641 ctatatgggg tttagccgtc tgggtctggc gattggcgac gctattggtt atatcggtgg
    41701 cggctggctg tttgacctgg gcaaatcggt gcaccagcca gagcttccgt ggatgatgct
    41761 gggcattatt ggcatcttca ctttccttgc gctgggttgg cagtttagcc agaaacgcgc
    41821 cgcgcgtcgt ttgcttgaac gcgacgcctg atttgatact cccccctgac tcctccatac
    41881 tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg
    41941 cccttactgc ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca
    42001 tgttctgctc aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc
    42061 cattctgcaa aaagatgaca gccgctatat gccagaaagc atggatatcg ttcactatgt
    42121 cgataaactc gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg
    42181 gctgcgcaag gtcaatggct acgccaacaa actgctgttg ccgcgttttg ccaaatcggc
    42241 atttgatgag ttttctactc ccgccgcgcg caaatatttc gtcgacaaga aagaggccag
    42301 cgcgggtaat tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga
    42361 tgatttacgt gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc
    42421 ggaagatgat attcagctat tcccgctact gcgtaatctg acgctggtag ccggaattaa
    42481 ctggccaagc cgcgttgctg attaccgcga taatatggcg aaacagacac aaatcaattt
    42541 gttatcatca atggcgattt aatcctgact cgccgcggca gcctgtattc gcgggttgcc
    42601 gccgttttca gttttttctg ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc
    42661 aggatattga cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg
    42721 cattgatcgt cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg
    42781 aacaagaaat taaccagtcg cttgcgaaac ataataattt ctcaaaagat attggtttac
    42841 ccggtgtggc tgacgcccat attgttctga caaacctgac cagccaaatt ggtcgcgaag
    42901 agccgaataa ggttgcccta accggagacg ccaatctgga catgaactcc ctgttcggta
    42961 gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag
    43021 gcgcgatctt cctgaaagag atggaggtgg tcgatgcgac ggtacaaccg gaaaaaatgc
    43081 aaacggtgat gcaaacgttg cttccctatc tgaaccaggc attacgcaat tactttaacc
    43141 agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg cgaagcaatg gcgaaaaaac
    43201 tggcgaaagg cattgaagtg aagccaggcg aaattgtcat tccatttact gattaatcac
    43261 gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg
    43321 tgtccggcaa aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg
    43381 tattaaagat ccgccgccca gacgactggc accttcacct ccgcgatggc gacatgttaa
    43441 aaactgtcgt gccgtatacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg
    43501 ctccgcccgt gaccaccgtt gaggctgccg tggcgtatcg ccaacgtatt cttcacgccg
    43561 tacctgccgg gcacgatttc accccattga tgacctgtta tttaacagat tcgctggatc
    43621 ctaatgagct ggagcgcgga tttaacgaag gcgtgttcac cgctgcaaaa ctttacccgg
    43681 caaacgcaac cactaactcc agccacggcg tgacgtcagt tgacgcaatc atgccggtat
    43741 tagagcgcat ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg acacatgcag
    43801 atatcgacat ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc
    43861 agcgcctgac cgcgctgaaa gtcgtttttg agcacatcac caccaaagac gcagccgact
    43921 atgtccgtga cggaaatgaa cggctggctg ctaccatcac tccgcaacat ctgatgttta
    43981 accgcaacca tatgctggtt ggtggcgtgc gtccgcacct gtattgtcta cccatcctca
    44041 aacgcaatat tcaccaacag gcattgcgtg aactagtcgc cagcggtttt aatcgagtat
    44101 tcctcggtac ggattctgcg ccacatgcac gtcatcgcaa agagagcagc tgcggctgcg
    44161 cgggctgctt caacgcccca accgcgctgg gaagttacgc taccgtcttt gaagagatga
    44221 atgctctgca gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc tatgggttac
    44281 cggtcaacga cacattcatc gaactggtac gtgaagagca tcaggttgct gaaagcatcg
    44341 cactgactga tgacacgctg gtgccattcc tcgccgggga aacggtacgc tggtctgtta
    44401 aacaataaaa atcgtagctt cctgttgtca ttaggttatt ttacctgtat aaataaccag
    44461 tatattcaac agggggctat tatgcgaatt gaagtcacca tagcgaaaac ttctccattg
    44521 ccagctgggg ctattgacgc cctggctggc gaactttccc gccgtattca gtatgcgttt
    44581 cctgataatg aaggccacgt atcagtacgt tatgccgcag cgaataattt atcggttatt
    44641 ggcgcgacaa aagaagataa acagcgcatt agcgaaattc tccaggaaac gtgggaaagc
    44701 gccgatgact ggtttgtcag cgaataatat gcagtgattt ttgtttttgc cgggtcgccc
    44761 cggctttttt attttacttg cgaattatga gtacaaaatt tctacacttc ctcaaaataa
    44821 ttgcacaata tgctaaaaaa atatgaatga catgctgttt tattgttcat ataaatctta
    44881 a
//
LOCUS       DAZKFZ010000012        44511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000012 DAZKFZ010000000
VERSION     DAZKFZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            229..573
                     /locus_tag="ACHOQW_000664"
     CDS             229..573
                     /locus_tag="ACHOQW_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP9571791.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(577..2166)
                     /gene="yejF"
                     /locus_tag="ACHOQW_000665"
     CDS             complement(577..2166)
                     /gene="yejF"
                     /locus_tag="ACHOQW_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP9571792.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(2168..3193)
                     /locus_tag="ACHOQW_000666"
     CDS             complement(2168..3193)
                     /locus_tag="ACHOQW_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9571793.1"
                     /translation="MSRLNPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(3193..4287)
                     /locus_tag="ACHOQW_000667"
     CDS             complement(3193..4287)
                     /locus_tag="ACHOQW_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP9571794.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(4288..6102)
                     /locus_tag="ACHOQW_000668"
     CDS             complement(4288..6102)
                     /locus_tag="ACHOQW_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9571795.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(6184..7740)
                     /locus_tag="ACHOQW_000669"
     CDS             complement(6184..7740)
                     /locus_tag="ACHOQW_000669"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9571796.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(7921..8487)
                     /gene="mepS"
                     /locus_tag="ACHOQW_000670"
     CDS             complement(7921..8487)
                     /gene="mepS"
                     /locus_tag="ACHOQW_000670"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP9571797.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(8900..9613)
                     /gene="lpxT"
                     /locus_tag="ACHOQW_000671"
     CDS             complement(8900..9613)
                     /gene="lpxT"
                     /locus_tag="ACHOQW_000671"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP9571798.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(9652..10638)
                     /gene="yeiR"
                     /locus_tag="ACHOQW_000672"
     CDS             complement(9652..10638)
                     /gene="yeiR"
                     /locus_tag="ACHOQW_000672"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP9571799.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKNPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(10756..12222)
                     /locus_tag="ACHOQW_000673"
     CDS             complement(10756..12222)
                     /locus_tag="ACHOQW_000673"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9571800.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            complement(12445..13017)
                     /gene="yeiP"
                     /locus_tag="ACHOQW_000674"
     CDS             complement(12445..13017)
                     /gene="yeiP"
                     /locus_tag="ACHOQW_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP9571801.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            13172..13426
                     /locus_tag="ACHOQW_000675"
     CDS             13172..13426
                     /locus_tag="ACHOQW_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9571802.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(13423..14604)
                     /gene="setB"
                     /locus_tag="ACHOQW_000676"
     CDS             complement(13423..14604)
                     /gene="setB"
                     /locus_tag="ACHOQW_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP9571803.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            14972..16102
                     /gene="fruB"
                     /locus_tag="ACHOQW_000677"
     CDS             14972..16102
                     /gene="fruB"
                     /locus_tag="ACHOQW_000677"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP9571804.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVVEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            16102..17040
                     /gene="fruK"
                     /locus_tag="ACHOQW_000678"
     CDS             16102..17040
                     /gene="fruK"
                     /locus_tag="ACHOQW_000678"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9571805.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            17057..18748
                     /gene="fruA"
                     /locus_tag="ACHOQW_000679"
     CDS             17057..18748
                     /gene="fruA"
                     /locus_tag="ACHOQW_000679"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP9571806.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            19172..20113
                     /locus_tag="ACHOQW_000680"
     CDS             19172..20113
                     /locus_tag="ACHOQW_000680"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP9571807.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            20101..21018
                     /gene="psuG"
                     /locus_tag="ACHOQW_000681"
     CDS             20101..21018
                     /gene="psuG"
                     /locus_tag="ACHOQW_000681"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP9571808.1"
                     /translation="MSELKISPEVQDALKNKKPVVALESTIISHGMPFPQNAQTAIEV
                     EETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNG
                     ATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKS
                     ILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARAMAVKWQS
                     GLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLARVAELTGG
                     DSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            21112..22362
                     /locus_tag="ACHOQW_000682"
     CDS             21112..22362
                     /locus_tag="ACHOQW_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9571809.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(22433..23131)
                     /locus_tag="ACHOQW_000683"
     CDS             complement(22433..23131)
                     /locus_tag="ACHOQW_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP9571810.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            23261..24202
                     /gene="rihB"
                     /locus_tag="ACHOQW_000684"
     CDS             23261..24202
                     /gene="rihB"
                     /locus_tag="ACHOQW_000684"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP9571811.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            24302..25552
                     /locus_tag="ACHOQW_000685"
     CDS             24302..25552
                     /locus_tag="ACHOQW_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9571812.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(25608..26696)
                     /locus_tag="ACHOQW_000686"
     CDS             complement(25608..26696)
                     /locus_tag="ACHOQW_000686"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9571813.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(26699..27556)
                     /gene="nfo"
                     /locus_tag="ACHOQW_000687"
     CDS             complement(26699..27556)
                     /gene="nfo"
                     /locus_tag="ACHOQW_000687"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9571814.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(27630..28679)
                     /locus_tag="ACHOQW_000688"
     CDS             complement(27630..28679)
                     /locus_tag="ACHOQW_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP9571815.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            28778..29659
                     /gene="yieE"
                     /locus_tag="ACHOQW_000689"
     CDS             28778..29659
                     /gene="yieE"
                     /locus_tag="ACHOQW_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP9571816.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            29864..31333
                     /gene="lysP"
                     /locus_tag="ACHOQW_000690"
     CDS             29864..31333
                     /gene="lysP"
                     /locus_tag="ACHOQW_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP9571817.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            31625..33604
                     /gene="cirA"
                     /locus_tag="ACHOQW_000691"
     CDS             31625..33604
                     /gene="cirA"
                     /locus_tag="ACHOQW_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP9571818.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRNGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33636..34472)
                     /gene="yeiG"
                     /locus_tag="ACHOQW_000692"
     CDS             complement(33636..34472)
                     /gene="yeiG"
                     /locus_tag="ACHOQW_000692"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9571819.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            34730..35398
                     /gene="folE"
                     /locus_tag="ACHOQW_000693"
     CDS             34730..35398
                     /gene="folE"
                     /locus_tag="ACHOQW_000693"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP9571820.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            35415..36572
                     /gene="yeiB"
                     /locus_tag="ACHOQW_000694"
     CDS             35415..36572
                     /gene="yeiB"
                     /locus_tag="ACHOQW_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP9571821.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            36714..37754
                     /gene="galS"
                     /locus_tag="ACHOQW_000695"
     CDS             36714..37754
                     /gene="galS"
                     /locus_tag="ACHOQW_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP9571822.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            38033..39031
                     /gene="mglB"
                     /locus_tag="ACHOQW_000696"
     CDS             38033..39031
                     /gene="mglB"
                     /locus_tag="ACHOQW_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP9571823.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            39092..40612
                     /gene="mglA"
                     /locus_tag="ACHOQW_000697"
     CDS             39092..40612
                     /gene="mglA"
                     /locus_tag="ACHOQW_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP9571824.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            40628..41638
                     /gene="mglC"
                     /locus_tag="ACHOQW_000698"
     CDS             40628..41638
                     /gene="mglC"
                     /locus_tag="ACHOQW_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP9571825.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(41881..43116)
                     /gene="preA"
                     /locus_tag="ACHOQW_000699"
     CDS             complement(41881..43116)
                     /gene="preA"
                     /locus_tag="ACHOQW_000699"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP9571826.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(43110..44348)
                     /gene="preT"
                     /locus_tag="ACHOQW_000700"
     CDS             complement(43110..44348)
                     /gene="preT"
                     /locus_tag="ACHOQW_000700"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP9571827.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT        11005 a        10923 c        11615 g        10968 t
ORIGIN      
        1 tgtaaaaagt cacatcattg tagttaaaaa ggttgagtta gatcgcagaa acgggtacat
       61 atagccccgc aaacgtgacc acgcccgcag atattactta aatcagagcc atagaggcca
      121 cgcaggcgag gcatcaatct ttacgatctg tataaagacg gattgttgat gatgtgttaa
      181 aattgatgta aacaaattgt gaagtaaatg tgcttccggg gaaaataagt gacttcatta
      241 caactctcaa tcgtccatcg actgccgcag aactatcgct ggtctgctgg tttcgcaggt
      301 tcgaaggttg aaccgattcc gcaaaatgga ccgtgcggtg acaacagcct ggtggcgctt
      361 aaattgctta gcccggatgg tgataatgca tggtcggtga tgtataaact aagccaggcg
      421 ttaggcgaca tcgaagttcc atgttcggtg ctggagtgtg aaggtgagcc gtgcctgttt
      481 gtaaatcgcc aggacgagtt tgctgcaaca tgccgattga aaaattttgg tgtggcaatt
      541 gccgaaccct tttcaaacta caatcctttt taagcgtcag ctcaacgcca gtagctgacg
      601 cgtatactcc tgctgcggtg tggcaaacac gcgcgcgcac ggtccttgtt ccactacttc
      661 cccttgtcgc agtacgataa cctgatgaca taacgcgcgg acaacgtgca aatcgtggct
      721 gataaacaaa taggccagtt gatgcttttg ttgcaatgat ttcaatagcg tcaatatttg
      781 cgcctgtacc gttttgtcga gtgatgatgt cggttcatca agtatgatca gcgagggctt
      841 aagaattaat gccctggcaa tcgcaatacg ttgtcgctga ccaccagaga actccgccgg
      901 ataacggtgg cgtgtttcag gatctaatcc cacttcatgc atcacgtcta tcacttgttg
      961 ttcgcgttgt gcggcagaaa gcgtcggctg gtgaacccgt aagccttcct caataatctg
     1021 caaaacgttg agtcgtgggt tgagcgagga gtttggatcc tgaaatacca cctgaatgcg
     1081 atgacgaata ggtaacagct ggcggcgatt taaattttgc agtggctgac cgtcaaagat
     1141 gatgctgccc tgagaattaa tcagtcgcag cagcgccagt cccgtcgtgc ttttcccgga
     1201 accggactcg cccactaaac ccagtgtttc acccgctcgt agcgtaaaac tgatgttttt
     1261 caccaccaca ttatgatcca caatgcgctt caaaatccct ttgcgaatgg ggaaggcaac
     1321 ctgaagctgt tcaacatcca gcaacgttga ggcaggttct ggcaacggca ctggatcgcc
     1381 tgacggttca ctgttgagta gcttttgtgt gtaaggatga gtgggtgagg caaatagcgt
     1441 agcggcgtaa ttttgctcga cacagcgacc gttttgcatt accgccacgc ggtgggccag
     1501 ttttctgaca atgctgaggt tatgagtaat aaacagcatg cccatattca actcgccttg
     1561 cagttcgcgc aacagctgta aaatctgcgc ctggacagag acgtccagtg cggtggtcgg
     1621 ttcatcggca attaataatt ccggtcgcgt taacagcgcc atcgcaatca tcacccgctg
     1681 gcgttcgccg ccggagagct gatgcggata atctgtcagc cgttttgccg cctggcggat
     1741 accaacgcga tcaaggcagt taagaatttc gccacgagcc gcttcccgac gcatcccgcg
     1801 gtggagtgaa agcacttcat aaagctgttt ttccagggta tgcaatggat ttaacgacac
     1861 catcggctct tgaaaaatca tggcgatctt attaccgcgt acaccgcgca acgtttggtc
     1921 gctggcatga agcagcgatt cgccatgaaa acgaatatcg ccggagagat attcaaccgg
     1981 cggggaaggg agcaggcgta aaattgacag cgcggtaacg cttttgcctg aacctgactc
     2041 acccaccagc gccagcgttt cgccagcctc aatctgtagt gaaacatcat tgactactgt
     2101 acgtacggtt tgctgatggc gaaaacccac cgacaaatct tcaatcgcta acagagtttg
     2161 cgtcatgcta caccgcctta ttaggatcaa atgcgtcgcg gacggcttca ccaataaaga
     2221 tcagcaaaga caataatatc gccaccgaca agaaggcggt gatcccaagc cacggagcct
     2281 gaaggttatt tttcccttgt aacagcagtt caccgagtga cggtgaaccg agcggcagac
     2341 cgaagccgag gaaatcgagt gaggtcaggg tggtgatcga actacataaa ataaacggta
     2401 aaaaggtgag ggtagcgacc attgcattcg gcaacatatg acgcagaata atactgcgat
     2461 cgctgacgcc aagcgcctgt gccgcgcgga tgtagtcgaa attgcgggta cgtaaaaact
     2521 ccgcccgcac cacgccgacc agactcatcc agccaaataa gacagtaatt gccagcagcc
     2581 accagaagtt aggttgcaca acgctggaaa gcagaataat caaaaacagc gttggcatcc
     2641 ccgaccatac ttcaataaag cgctgccccc agagatcgac cttaccgccg taatagcctt
     2701 gtagcgcccc cgccagcacg cccatcacgc tggaacagag agtcagcatc aggccaaaca
     2761 gaaccgagat ccgcgtgcca tagagaatac gtgccagcac atcgccgccg ttggcatccg
     2821 ttcccagcca gttttgccgg gaggggggag aagggaaggg cttatcggta gcaaagttga
     2881 tactggtagc accaaagcga atcggtgccc acagtaccca gccattattt tccagccgtt
     2941 gtttcagcca cgggtcctga taatcagcct gacttgccag cgggccgcca aaatcgcttt
     3001 cgctgtagtt tttcaacaac gggaaatacc aactgccgtc ataacgcacc agcaacggtt
     3061 tatcgttggc gataagttca gaacacaaac tcaaaccaaa caaaacgagg aaaatccata
     3121 acgaccagta gccgcgacgg ttatggcgaa aacgcgccca acgggcctga ttgacggggt
     3181 tgagtcgcga cattaacgtc cctcaaaatc tatacgcgga tcgaccagcg tatagctgat
     3241 atcactgaca atattcagca gcaggccaat cagggtgaaa atatacaagg taccaaacat
     3301 tacaggataa tcgcgcgaga cggtcgcttc gtagcccagt aagccaagac cattgagtga
     3361 aaacatcacc tcaatcagca gcgagccggt aaaaaacatg ctgataaacg tcgccggaaa
     3421 accggcaatc accagcagca tggcgttgcg gaacacatgt ttccagagaa tatttttttc
     3481 acttacccct ttcgcacgcg cggtcaccac gtattgcttg cgtacttcat caaggaatga
     3541 gttttttgtc agcatagtca gcgccgcaaa gccaccaatc accgtcgcca gcaccggcag
     3601 cgtgatatgc cacagataat cggtgatttt ctgataccac ggcagcgaat caaagttagc
     3661 ggaaaccagg ccgcgtagag ggaacaggtc gaaatagctg ccgcctgcga agaagacaat
     3721 cagcaggatg gcaaacaaaa aggccggaat ggcgtagccg atgatgataa atgcgctact
     3781 ccagacgtca aagcggctcc cattataaac agctttgcga atgcctaacg gaatcgacac
     3841 cagatagata atcagcgtgc tccacaatcc gagggtgatg gaaaccggca gactgtcttt
     3901 aatcagcgtc agcaccgagg cgctgcgaaa caggctatcg ccaaaatcaa agcggatgta
     3961 gtcccagagc attttgaagt aacgttcgtg gatcggctta tcgaaaccgt agcgatgagt
     4021 gatctcggcg atcacttctg gatctaatcc gcgtccgcca cggtaattac tgtcgctgat
     4081 attaccgacg cccgtttgcg catggctggc gcgaacgcct tcaccgcctg cgccgggtaa
     4141 tactccggca ttaccaaact caatggcggc gatggcctgg tcgaccggac cgccaggcgc
     4201 aatttgcacg atgaaaaagt tgatagtgat aatcgcccat aacgttggga tcaccagcaa
     4261 cagacggcga atcagataag cgcccatcta ctctccctgt tgtctggctg acggcagttt
     4321 ggccgcttta ttaacatcat accaccaggt atcgataccc agactgtaaa cagggcgcac
     4381 agcgggttgg gagaatttat cccaccaggc gagacggtct tccgccatgt accacattgg
     4441 cagcatgtaa taattccacg ttaatacgcg atccagtgct cgccctaacg gcagtaattt
     4501 ttctttattt ccctgcgcgg caataatttg gttgatcagc gagtcgataa ccgggctttg
     4561 cacgccgggg gcattataag tggaattgat atattccgat gaccaggaaa tctgtaaatc
     4621 ggaactgggc cacggcatcg cccgccatag gcgcggcatc atgtcatagt cgcgactgcg
     4681 catgcggttg gtgatttgcg agtgatccac cttgcgaatg tccatgttga tacccagccg
     4741 ttgcaggctg tgctggaacg gcaataccca ctgactattg ctgcttgcag gaagcaataa
     4801 ttcaaagctg agcggctgac ccgtagtggc attaacgcgt tgctgacctt tcagcaccca
     4861 gcccgcttcg ttgagaagtt tgtcggcttt taacaggttg tcacgatcgt agccatcgcc
     4921 tttcgatacc ggcggctggt agatttgtgt gaagacttct ggcggtaaat cttttttcat
     4981 tggtgccaga agcaccagct ccgcggcgtc ggggtaattt ctggccgcgt attcggtatt
     5041 ctgaaaataa ctgttcgtgc gactccaggc attgtaaaac aacgccttgt tcatccattc
     5101 aaagtcaaag gcgagggtga ttgcttcccg gacccggcga tcgctgaata ccggacgttg
     5161 gatattaaac gccagccagc gcgtatcctg ggctgattcg ttcttttgct catctttgat
     5221 gatgtatttt ttatcgaaat ttttaccgct atagcgtgtg gcccagtttt tggcgtcgtt
     5281 ttccatacgc aaatcaaagg cacctgcttt aaacgcttca aaggcgacat tatcatcgag
     5341 gtaataatcg tagcgaatgg tgtcgaaatt ccagcgtcca cggtttaccg gtaagtttgc
     5401 tgcccagtaa tctttcacgc gggaatagac aatattttgc cccattttcc aggacgtaat
     5461 tcggtacgga ccactggcaa gcggaggcgt ggcgagcgga tcgctaagtt tgtgatcctt
     5521 ccagtacttt tctggaaata ccggcagcga aaacagactc aacatatctt ctttgccggg
     5581 tttagctaac tcaatgcgca cggttaacgg cgcaatggct ttgacggtgg tgcctttgta
     5641 gaccagacga aattgcggca cgccttcagt cataaatttt tgaaaagtaa actctacatc
     5701 gcgggcagta atgggcgaac catcatgaaa acgggcgcga ggattaatag cgacctccac
     5761 ccaggaataa tcgtcagcat agcgtgcgct ttcagcaatc agcgggtaat aactgcctgg
     5821 ttcgtcatcg gaagtcgtaa atagcgtgtc gtacagctgc tcggtgcgtg cgcccgggtt
     5881 gccgcgcagt gcatagcggt tgaaattatc gaaggtaccg agggctgaca acgttatctg
     5941 cccacctttt ggcgcggcgg ggttcacata atcaaaatgg ttgaaattaa acgcgtaccg
     6001 gggttcgccc agcacggcaa aggcatagct ttccttgata gcctgcgcct gcgcaccaaa
     6061 ggtgaacaga gcgataaaca gcagcagtat gcgcacaatc atctgcaacg gatttccttt
     6121 tactcatcat tagccgcaat ccggcatgct ttcactatga ataataaggg acgtaagcac
     6181 accttaccac tgcggcgtat acggtttctt cagccagcgg acaaaatcgt ccagcggtaa
     6241 cgggcgacta atccagtagc cttgcatgaa attaacgccg cgttcgctta gccatcgcgc
     6301 ctgttccggc gtttcgaccc cctcagcaac cgtcagcata ttgaggcgtt tcgccagcgt
     6361 cagcaccgcg tcaagtacgg gtgaagtgat cgtttccgta ccgatggcgt tgataaatcc
     6421 acggtcaatt ttcagataat cgagcgtaaa acgctcaaga tagataagcg cgctgtgccc
     6481 ggtgccgaag tcatcaatag caatttctac gccgaccgag tgcagccagg cgaagagttg
     6541 tgtggcttct tgctctttca gcatatcgcg ctcggtaatt tccagcacaa tctggaaatg
     6601 gtgtgcgggt agggaagtga gcagtttctg gatatttgct ttaaagcttt cgctgtgcag
     6661 atggtccggc gcaatgttaa tgccaaattt gacgcctacc ggcagcactt tttctaattc
     6721 tgcggcatcg cgggcaatta actcaaacag gtgctgagtc agcggcacaa tcatcttttg
     6781 cgattcggca aagttaatga aggcatccgg gggaatttct cccgcgacag gatgccgcca
     6841 gcgtagcagt acttccaggc ccgttactcg caaagcttgt gtatccacca ccggttgata
     6901 cgccacgtaa aattgttcgc gtttgatggc ggtcatgatt tctctgccgg ggcgcatacg
     6961 tacgctcatc aggtaatagc agagcaggcc aacgacagtt ccactcatgc cgcccagtaa
     7021 aaatgcgtac cacacatcgt tccatgtcca gtcatctgca taaagccgaa cggtcagagg
     7081 aatgcccgca attttagttt cacggactgg catgtcggtt aattcgttaa cgttcatcaa
     7141 acgtgaagaa aaggtagata gcgcagtatt gccaataatg agggcgacgc catcgtaatc
     7201 ttcctgccgt gagctataaa agagagaagg cgtcaggttg agattcagag cggcaaagac
     7261 gccgctattt ttcagcaaag ggttgcgata ccagatgacg attgcgggtt tgttcggcac
     7321 catcggcgtg ccgggtaaga tcgccatatc gacattttta ttaatgtcga gcgcaggaat
     7381 caattcattg agtggaatgt ccatctcacc ggtcgcagat gaacagaatg tttttttatc
     7441 tttcaccagc acaaacgttc ggacattcat gctaaacgcc gcgcgggcgg tcagttcggg
     7501 gttagcttgc ttgcatgtat ctaaggtcag cggctggagc cggtcagtag tggatttcag
     7561 gtcggcaaaa taggtatcga gatacttttg tacgtcggta atcagtgtgt cgtatttgac
     7621 ttcgtgctta tgccaggcca ctaaaaactg aaggcagctc accagtatcg caatcattac
     7681 gcctgcaaca atgcaggtaa gcaggagctt acgtccagaa ttgggagcgc gtatgaacat
     7741 gattccgtta atttcctgaa attacgacaa catttaaaag ataacaatga ccggactata
     7801 gcctggatca agacgcggaa acatcatttt ctctgatgaa tgaaaacaaa aaagcactgc
     7861 ctaaacagtg ctttcagtta actcgtcagg atagccaagg gattgcatcc aaacggttta
     7921 ttagctgcgg ctgagaaccc ggcgtgcttc gttgtaacgc ttcttccagt acggttcatt
     7981 catgctggaa ataataacac cactgctggt ggaagcatgg acaaactgat tgttgccgat
     8041 ataaataccg acatggcgtc ccgttgaacc ggcacggaac agaactaaat cacccgtacg
     8101 caaattactg cgggaaacag atttacccat ttcctgctgt tcgtaagtcg aacgcggaag
     8161 ttctaagcca aattgctcac ggaatgtacg ctgtacgaaa ccagaacaat cgataccttt
     8221 tttagtgctg ccgcccagac gataacgtac gcctttccag tcagcatact gatccataat
     8281 tcgcgatttt acgtcgacat tacgaaccag gttttcaaat tcatcctgag aagcttgcag
     8341 tgatgatgtt tcactaccca ctgcacgtgt ctcaggatgc atattctttg cggtgttatt
     8401 tgcactacat gcagaaagca gaaccgctac tgcaatcgcg gggatcccgc gcaagatata
     8461 tctcaaaatc ggttgagatt tgaccatgtt gtttgttttc ccttgaagtc cttaacgaca
     8521 aatatcgtta taaaaaaaat gccaagcgac acgagactaa ttaccagggc atgttgaaac
     8581 aattccttaa ggccaaatct gtggactaac gcacaaaatt atatgctgtg aggataatct
     8641 accgccgagg caaaacgagt tcgagattac ccgattggtg cggtcttggc gagcggtttt
     8701 acatgatttt ttataagaaa aagtgataca gaaagttaat aagcgggatt ggtcaattaa
     8761 agtacgattg agtgaaatgc atgcaatcca ttcatttact tagagaatga tttttgcttt
     8821 cgatggatga aagcacggtg cgcatcatca ggattatcct cactataaaa ataaacctga
     8881 tgatgttaag tactgtgagt tatttgtttt ggaaatgttt gttttttccc ggtagtgatt
     8941 tgtcaaaaaa ggtgattaat cgatcactta atggcgtcag caacacccag ggcaaaccga
     9001 tcaatatcac ggtcatcgaa ccgacaatga tgtcagtaaa ccagtgtgcg ccaatcatta
     9061 ctctgggaaa cgcaaaaacc acaaaaataa taagggcgat aagacctgca actttgccga
     9121 aataacgcca catgaatgcc gaaaaaataa gcagcatcat gccgtgatcg ccgggaaagc
     9181 tatctcgtga ggcatctttc gtggggacag agagcagttc gctgacgcgg ttaatatcgg
     9241 taaaagtcaa tgttgggctg gcccgtttta caggaattaa tgcctgaccc agctggttta
     9301 ataccactgc agttaatagc atgaccagac caataatcac gatacgtcgt ctgcctgggg
     9361 cgttttcttt cagccagaaa ctcagcatca acattcccat tgccagcagt gaacaaccgt
     9421 cgaaagcgcg attgttggtc aatgcaacca gccacaaaaa ggccttactt tcgaccagtt
     9481 tctgattaaa gaaataaaaa atatccgcat caatcggcaa ccagaatcca tgattaacgg
     9541 gaatatacca ggaaagaaac agcgcgaggc cgacaatatt caacaacact atttgcggca
     9601 aatttttaat catattttct ctaattatct tactgaaaaa cgcaggcaac cttacgcggt
     9661 agtcgctaaa cgaagcttca acagcgcgct ctgcaaggca ttccagtcag cttcgctgct
     9721 ggaaatcagc tcaatacggc tgtccggtgg cgcaacgttt tgcgtttcaa tgtgcaggtc
     9781 atcgccctga cggttgattc gcaccagccc ttcgggaata cgcagcacgc ctttgacgcg
     9841 ttccaccggt gcaagtcgcg cccattccag aatgccaatg gtgtcgaata ccgtatcagc
     9901 gtcgaatatc cagccgcagg cctgatgtcc ttgtccactg ttcagactgc gacgccagcg
     9961 ttgatgctct ggcaggctta acgctgctaa cccttttttc acgacatgct gatgagaatg
    10021 cgcggcgctg gcgggcaact cggctaaatt gtgacgcggc aaatccagaa gatgaccggc
    10081 aactttccca tgctcactgt gaattaattg ccgatcgcca ccattttgct gccaccagcg
    10141 ctgtagcgct tgctcgcttt caggtgtcgc acggtcggat ttattggcga caatgatgtc
    10201 tgcggcagcc agctggtcac ggaagttttc attgctggcg cttttttcgt ccagcagcag
    10261 gcgtggatcg agaatgcaca acgtggcgcg cagatctatc cacggttcat agactggtgc
    10321 ggttaacaga tcgaggatct gtttcggatg gcccagccca gtcggctcta tcaacaagcg
    10381 gtctggtttt ccctgacgca gtaaggtatt caaccctacc tgcatgggta aaccattaac
    10441 gcagcacatg cagccgccgg ggatctcttt cagcagtgcg ccgctatcgg cgagcaaagc
    10501 accatcaatt ccgacttccc caaattcatt aaccagtacc gcccattttt cgttgggatt
    10561 tttatgggct aacagatgaa gaatcgacgt ggttttcccg ctgccgagaa aaccggtgat
    10621 gaggttggtc ctggtcacaa cagctccgca atactctggt aagttaactg agcgttaatc
    10681 ctggctaaaa acggcagaaa agagaagggg agagcggacg aaggcgagct tcatccgctt
    10741 taaccgcaga aatcgttaaa ttttgagggt gtttaatagc gcctgatgcg cgccgaattg
    10801 tgcgatttgt tgccgggctt gttcgatggc ctgcacaaaa tgcgggttat ctggcagatc
    10861 atcaccgaaa acttcacgca gggaaagcag ggcggtgacg cgttgctcac tgatgctgcc
    10921 cgcaacaaga tcgcgaattt tatcgctaag cggatcgcga acatcaatgg catttccggc
    10981 atcatcaacg ccgctgacgt aacgcatcca gcctgcaacg cctaatgcca gcaacgacca
    11041 gtccgtttcg cgccccagat gtatgcgaat acctgccagc atgcgttgcg gtaatttctg
    11101 gctgccatcc atcgcgattt gccaggtctt atgtttcagc gccggattag caaaacgtgc
    11161 aattaactta tccgcatatt gtgttaaatc gacatcttta atccgcagtg tcggcgcttg
    11221 ctcatccagc attaatgttc tggcggcatg gcgaaatgcg cgatcctgca tgcaatcact
    11281 gatatgggcg aatcctgaga ggtaacccag ataagcgaga aaagagtggc tgccattaag
    11341 catccgcagt ttcatctctt cccatggcag gacatcattc accatttgta cacctgcgac
    11401 ttcccaggca ggacgcccag cgacgaagtt atcttccacc acccactgga taaacggttc
    11461 gcagctaatc gcgcagggat catttacccc cagatgctgg ctgatttctg ccagtgattc
    11521 gtcggttgca gccggaacaa tgcggtcgac catggttccc ggaaaactga cgtgttcttt
    11581 tatccacccg gcgagttctg gcgaacgttt ttctgccatt cccagcaccg cgtttttcac
    11641 cacatggcca ttgtcgggaa tattgtcgca ggagagcacg gtaaacggtg taaggccgcg
    11701 ctcacggcgg cgtttcagtg cttcgacgag aatacccggt gcggagtgag gttcttcagg
    11761 ggtttgtaga tcgtgaataa tccgcggatt gctggtgtcg agtgcaccgg tggccgggtc
    11821 aatacaatag cctttttcgg taatcgtcag ggaaacaatt gccacctgtg gctcgcaaaa
    11881 tttctcaata attgccgcta aggaatccag ttttgcattc aggcattcgt gaacggcacc
    11941 gacaattatc gcctgattgc cgtccgcccc tttctccagc acggtatata aatggttctg
    12001 tgcgcggagc tggctcatca gttgatcacc gctgaacaag ctgatttcac agatccccca
    12061 gtcgccgccc tgggcattca gcacacgatc ggtcagtaac gcctgatgtg cgcggtgaaa
    12121 ggctccaaag ccaaaatgaa cgatacgtga ttgcaactgc tggcgatcgt agcgtggagc
    12181 gtgtacatga tgcgggagcg taacggaggc gattgtattc ataggagtca tgtcttagta
    12241 acgattaatt aacaatgaca gtgtaaagtt atatgacagc aattgaaatt ggtctggatt
    12301 gttaatgcga tgaagtgtga gggcgatcaa tcaacgagtg aaaattgagg gcgtaaggtg
    12361 ttcaggaagc gcaaaataga agattggtat aataaattaa aaaagggaca gcctgagcta
    12421 tcccttttct gcatgcggct gaagttagtc agcacgcccc atataacggc gttcttcgat
    12481 atggatacga attttttcgc ccgggcttaa gtattctggt acctgaatca ccagaccagt
    12541 gctcaatgtc gccggtttgt tacgggcgct ggcagatgcc cctttgatgc ctggtgcggt
    12601 ttcaacgatt tccagatcaa cggtctgcgg cagctcaagc gccagcagtt gaccgtccca
    12661 ggtcagcacc tgcatgtccg gcatgccgcc ctctggcata aacagcaact cttcttcaat
    12721 ctgatctttg gtgaaggtat acggggtata gtcttcttta tccataaaga catattcgtt
    12781 gccatcgaca taggagaagt caacgtaacg gcgggtcagc gtcacggtgt caacgatatc
    12841 atctcctttg aaacgctctt ctactttcag cccggtacgg acatcagaaa aacgcatttt
    12901 gtacagcgta gcggcaccgc gggcagtggg cgactgaata tcaatgtcct taaccagcag
    12961 cagtttgccg ttgtaattca gtaccatacc ttttttaatt tcgttcgctc ttggcatcga
    13021 aaaattcctg tagtcgaaaa tgtcaaaaat atcgcgacaa agtactcgcg acgagccttt
    13081 cgggcaagtg gtattcgcac ttttgctggt gcaaaaaagg tggtactgtg cgcgctcatc
    13141 aatccggtgg ttaaccttaa gagaacaacg tatggaatgc cgtccgggtt gcggggcgtg
    13201 ttgcactgcc ccttcaattt ccagccctat tcccggtatg ccagatggca agcccgccaa
    13261 tacgccctgc attcagcttg atgaacagca acgctgtaaa attttcacct cgccactgcg
    13321 gccgaaagtc tgtgcaggct tacaagccag cgctgaaatg tgcggtaact cgcgtcagca
    13381 ggcaatgacc tggcttatcg atctggaaat gctgaccgcg ccttaaacat ctttaatccg
    13441 cagtaagcaa aacagagtgg cgataatcat caccatcgca aaccagaaca cagcgtgata
    13501 attccagatc tcggcgacga tgccagccac tgatcccgcg atgatccagc ccacgcgcga
    13561 cgtgttggta tagagcgtgg tggctgaacc tgcctgaccg ggcatcagat cctgaaaata
    13621 gagcatcccg ataccgccca gaatgccaat aaaaatagca tttagcagct gcaagcccaa
    13681 cagaatgaca ggagaatgcg ccatcagcat tcctgcgtaa aaacagacgc cacccacggc
    13741 agcaacgcgc attaagaaac gcttacccag acgtttggcg aaatatccgg caatcaacat
    13801 ggtcgggatt tccagcccgg cggcggtccc catcatcaca ccggccagtt tctcgggaag
    13861 atggagttcg ttgataataa atagcggcat gttgatgatg tacaggctgt tcgagcccca
    13921 catcaatgta caaatgacaa acagcagcag cgtatcgcga cggttacggc gcggcgcttc
    13981 aaccgtgccg gtcgccagcg gaagctcttt ttgcatcgac ggtaaaaaca gccacaccat
    14041 cacgccgcaa acaataaatg ctaccgctgc gctcagatac attaccgtaa agctgaaacc
    14101 catcgctaag gcataagcca gcggtgggcc aatgacccat gccagtgaaa cctgagcgcg
    14161 taaaaaagag ctgaacatca ccgcctcacg tccggttttg tcggcatgtt cacgggcaag
    14221 ggcaaacatt tgcgggttag cggtcgagcc aaagctgcta agaaagacgc caacgaatag
    14281 caaaacaaag tagttgcgat tccaggcaaa aagggtgcag gccagcacgc ctaacaggca
    14341 gcaaaagaca atcagcgatt tgcgatcgcc gcgcttatca gagcgcccgg cgagaaactg
    14401 actcaccaga accccaatga cagcgctgcc ggtaaagaag aatcccacca tcgccggacg
    14461 ggcatgtact tcatcggtaa gaaaaatact gagtgtcggg gtttgcagag cgcccgcaat
    14521 accggtgaga aaggcaacga ttaaaaacgc cgtcgaggtc aggtcaaacg atttcgcgct
    14581 ggagactgcg ggggagttat gcatgctggt cgatcactgg aagaggaaga cgcggagttt
    14641 acgctgcttg tcagaaaata aacagacagc gaaatcaaaa gcgccacgaa taatcaaaat
    14701 gccatttcac tgttgctgat tggctgctga cgctgctgaa atgaaattgc tgataacgga
    14761 ttttcccatc agcaattagg aaaaatgaca aaaaattgtg cagcacatca aacttttgct
    14821 cataacttta cggctttcct tgcgtgctga aacagaagta ttatgctttc ttgaaacgtt
    14881 tcagcgcgat cttgtcttta accctaagcc aggttggcgc tttttttctc atagaagctg
    14941 aatcgtttca attcagcaag agaggagaac tatgttccag ttatccgtac aggacatcca
    15001 tccgggcgaa aaggccggag acaaagaaga ggcgattcgc caggtcgctg cggcgctggt
    15061 gcaggccggt aatgtagcag aaggctacgt caatggcatg ctggcgcgcg aacagcaaac
    15121 ctcaacgttc ctcggcaatg gtattgctat tccacacggc actaccgaca cccgcgatca
    15181 ggtgctgaaa accggcgttc aggtatttca gttcccggaa ggcgtcacct ggggtgacgg
    15241 tcaggtagcg tacgtagcga tcggtattgc tgccagctcg gatgagcatc tgggcctgct
    15301 acgccagctg acccacgtac tgagcgatga ttccgttgtt gaacaactga agtcagcaac
    15361 aacagcagaa gaacttcgcg cattgctgat gggcgaaaag cagagtgagc agctgaagct
    15421 cgacaacgaa atgctgacgc tggatatcgt cgccagcgat ctgctgactc ttcaggcgct
    15481 gaacgctgcg cgtctgaaag aggcgggggc agttgacgcc actttcgtca ccaaagccat
    15541 caatgaacaa ccgctgaacc tcggacaggg tatctggctg agcgatagcg ccgaaggcaa
    15601 tctgcgtagc gcaattgcgg taagccgtgc ggcaaatgct tttgatgtgg acggcgaaac
    15661 ggcagccatg ctggtgagtg tggcgatgaa tgacgatcag cccattgcgg ttcttaagcg
    15721 tctcgctgat ttgttgctcg acaataaagc tgaccgcttg ctgaaagcgg atgcggcaac
    15781 gttgctggcg ctgctgacca gcgatgatgc gccgaccgac gacgtgttaa gcgcggagtt
    15841 tgtggtgcgc aatgaacacg gcctgcatgc tcgtccaggt accatgctgg tcaacaccat
    15901 taaacaattt aacagtgata ttaccgtgac aaaccttgat ggcaccggca aaccggcaaa
    15961 cggacgtagt ctgatgaaag ttgtggcact tggcgttaag aaaggtcatc gcctacgctt
    16021 taccgcccag ggtgcagatg ctgaacaggc gctgaaagca atcggcgacg ctatcgctgc
    16081 tggtcttggg gagggcgcat aatgagcaga cgtgttgcta ctatcaccct taatccggct
    16141 tatgaccttg ttggtttctg cccggaaatt gaacgcggcg aagtgaacct ggtgaaaacc
    16201 accggtctgc atgcggcggg taaaggcatc aacgtggcca aagtattaaa agacctggga
    16261 attgatgtca ccgttggcgg cttcctcggt aaagacaatc aggatggttt tcagcaactg
    16321 ttcagcgagc tgggcattgc caaccgtttc caggttgtac aggggcgcac ccgaattaac
    16381 gttaagctga cggaaaaaga cggcgaagtg accgacttca acttctcggg ttttgaagtc
    16441 acccccgccg actgggaacg ctttgtgact gattctctga gctggctcgg tcagttcgat
    16501 atggtctgtg tcagcggaag cttaccgtca ggcgtcagcc cggaagcgtt caccgactgg
    16561 atgactcgcc tgcgtagtca gtgcccttgc attatctttg atagtagccg tgaagcgtta
    16621 gtagcaggtt tgaaagcggc accgtggctg gtgaaaccta accgccgcga gctggaaatc
    16681 tgggcaggcc gtaaactgcc tgaaatgaaa gatgtgattg aagctgcgca tgcgctgcgt
    16741 gaacaaggta ttgcgcatgt cgttatttca ctgggtgccg aaggcgcgct ttgggttaat
    16801 gcctccggcg aatggatcgc caaaccaccg tcagtcgatg tcgtaagcac cgttggcgca
    16861 ggggattcta tggttggtgg cctgatttat ggcttgctaa tgcgtgaatc cagtgaacac
    16921 acactgcgtc tggcgactgc tgttgcagcc ctggcggtaa gtcaaagcaa tgtgggtatt
    16981 accgatcgtc cgcagttggc cgcaatgatg gcgcgcgtcg acttacaacc ttttaactga
    17041 cagcaggaga ggcataatga aaacgctgct gattattgac gctaatctcg gtcaggcacg
    17101 cgcctatatg gcgaagaccc tgctgggcgc ggcggcgcga aaagcaaaac tggaaatcat
    17161 cgacaatccg aacgacgcag aaatggcgat tgttctcggt gattccatcc cgaatgacag
    17221 cgcgctgaac ggtaaaaatg tctggctggg cgatatttcc cgggcagttg cgcaccctga
    17281 gctgttcctg agtgaagcca aaggccatgc gaaaccttac actgcgccgg tcgctgcgac
    17341 agcaccggtt gccgccagcg gtccgaaacg cgtagttgcg gtgactgctt gcccgactgg
    17401 cgtagcacac acctttatgg cggctgaagc cattgaaacc gaagcgaaaa aacgtggctg
    17461 gtgggtgaaa gttgaaaccc gtggttctgt tggcgcgggt aatgcaatca ctcccgaaga
    17521 agtagccgca gcggatctgg tgattgtggc ggcagatatc gaagtggatc tggcgaaatt
    17581 tgctggtaaa ccgatgtatc gcacctctac cggtctggcg ctgaagaaaa ccgcgcagga
    17641 actggataaa gcggttgctg aagcaacgcc gtatgaaccg gcgggcaaag ctcaaacggc
    17701 gacctctgaa ggtaagaacg agagtgcagg cgcataccgt cacttgctga cgggcgtctc
    17761 ttacatgctg ccgatggtcg ttgcaggtgg tctgtgtatc gcgctttctt ttgcttttgg
    17821 tatcgaagcg tttaaagagc cgggtacgtt ggcagcggcg ctgatgcaga ttggtggtgg
    17881 ttcagccttt gcgctgatgg tgccggtact ggcaggttat attgccttct ccattgccga
    17941 tcgtccgggt ctcacgccgg gtctgattgg cggtatgctg gcggtcagca ccggttctgg
    18001 cttcattggc ggtattattg cgggcttcct ggctggttac attgcgaagt taatcagtac
    18061 gcaactgaaa ctgccacaga gtatggaggc gctgaaaccg atcctgatca ttccgctaat
    18121 ttccagtctg gtggtcggtc tggcgatgat ctacctgatc ggtaaaccgg ttgctggcat
    18181 tctcgaaggg ttgactcact ggctgcagac catggggact gcgaatgcgg ttctgctggg
    18241 ggcgatcctc ggtggcatga tgtgtactga catgggcggt ccggtaaata aagcagcgta
    18301 cgcattcggt gtgggtctgc tgagtactca aacctatggc ccgatggcgg cgattatggc
    18361 ggcaggtatg gtgccaccgc tggcaatggg tctggcaaca atggtggcgc gtcgcaaatt
    18421 tgacaaagcg cagcaggaag gtggcaaagc cgctctggtt cttggtctgt gctttatttc
    18481 ggaaggtgca attccttttg ctgctcgtga tccgatgcgt gtgctgccgt gttgtatcgt
    18541 tggtggggcg ctgactggcg caatctcaat ggcgattggt gcgaaactga tggcaccgca
    18601 cggtggtctg tttgttctgc tgatccctgg cgctattacg ccggtactgg gttacctggt
    18661 agcaattatt gccggtacgc tggtggcggg tttggcctat gccttcctga aacgtccgga
    18721 agtggacgca gtagcgaaag ccgcgtaata aaaggtgtgt tacagggcag aaatttactg
    18781 ccccgtatca ttggatgagc gcaaagagaa tgaccggttt gtgaatgtaa ttttatgaat
    18841 ttaaatggtt ttatctttca gtagaaacct gaaacgttca tcaaacacta aggttatttc
    18901 ctcaagggca gattcttttc tgcccttttt tagtgttcat gttctgtgca tcccgtcaca
    18961 aattcctcgc ttttgtaatt caggcattta atcgcatttt gtgagcctgc tcaaatctgc
    19021 ccgctttgcc tctctggcgg caatttactt tacgtaaaac atgcatacta tgagcacatg
    19081 tttaaatatg aacacatgtt taaaccgtct ttgcagtatc tatatccttg tggattgaca
    19141 gggttggtaa ttttttacca gaggacgtgc tatgcgcgaa aaggattatg tcgtaattat
    19201 aggctcggcg aatattgatg tcgccggata ttcacatgaa tcattaaatt atgcggattc
    19261 aaatccaggt aaaataaaat ttacgcctgg tggggtaggg cgcaatattg cacaaaacct
    19321 ggcgttgctg ggtaataaag cctggctact gagcgccgta ggcagtgatt tttatggcca
    19381 atcgctgcta acgcaaacca atcaatctgg cgtttatgtc gataaatgcc tgattgtgcc
    19441 gggagaaaat acgtcgagct atttatcatt actcgataat accggtgaaa tgctggttgc
    19501 tataaatgac atgaatatta gcaacgctat tacagctgaa tatctcgcac agcaccgtga
    19561 atttattcag agggcaaagg tcattgtcgc ggactgtaat atcagtgaag aggcactggc
    19621 atggattctg gataatgctg ccaacgtacc cgtatttgtc gatccggttt ccgcgtggaa
    19681 atgcgtcaaa gtacgcgagc gattaagtca aatccatact ctcaagccaa accgccttga
    19741 agcggaaacc ctgagtggga ttgcgctgtc agggcgtgaa gatgtggcaa aagttgctgc
    19801 ctggttccat caacatggcc tgaaccgact ggtattgagc atgggcggcg acggcgttta
    19861 ttacagcgat atcagcggtg aaagtggctg gtctgcgccg atcaaaacca atgttattaa
    19921 tgttaccgga gcgggcgatg ccatgatggc gggacttgct tcgtgttggg tagacggaat
    19981 gccgtttgcc gaatctgttc gtttcgcaca gggatgttcg tcaatggcgc tctcctgtga
    20041 atacaccaat aaccccgatt tatcgattgc caacgttata tcgttagtgg agaacgcaga
    20101 atgtctgaat taaaaatttc cccggaagtg caggacgctt taaaaaacaa aaaaccggtt
    20161 gtggcgctgg aatcgaccat tatttctcac gggatgccgt tcccacaaaa tgcccagacc
    20221 gcaattgaag tagaagaaac tattcgtaaa cagggcgcag tacctgccac tatcgccatt
    20281 attggcggcg tgatgaaagt gggtttaagc aaagaagaaa ttgaattact gggtcgtgaa
    20341 gggcataacg tgactaaagt tagtcgtcgc gatttacctt ttgtcgttgc cgcaggaaaa
    20401 aatggtgcaa ccaccgtggc ttcaacgatg attattgcgg cgcttgccgg aattaaagta
    20461 tttgccaccg gcggaattgg tggtgttcat cgaggggcgg aacatacctt cgatatttct
    20521 gccgatttgc aagaactggc aaatactaat gtcaccgttg tttgtgccgg ggcgaaatct
    20581 attctcgatt taggattaac cactgagtat ttagaaacct tcggtgtgcc gttaattggc
    20641 tatcagacta aagcgctgcc tgcgtttttc tgccgtacca gcccgtttga cgtcagcatt
    20701 cgtctcgaca gcgccagtga aattgcccgt gcaatggcgg tgaaatggca aagcgggctg
    20761 aacggtggcc tcgtggtagc gaacccgatc ccggaacagt ttgcgatgcc agaacacact
    20821 atcaatgcgg tgatcgatca ggcggtagct gaagctgaag cgcagggtgt tattggtaaa
    20881 gaaagtacgc cattcctgct ggcgcgcgtt gctgagctga ccggcggtga cagcctgaaa
    20941 tccaacatcc agcttgtgtt caacaacgcc attctggcga gcgaaattgc caaagaatat
    21001 cagcgtctcg cgggttaatt tctgacgggc agaaagccgt cgcccgtact catcaaacat
    21061 accgaaacct ggcgctgacg ccgggtttac tggcatgaag ggaaaatcat tatggacata
    21121 atgagaagtg ttgtggggat ggtggtgtta ctggcaatag catttctgtt gtcagtgaat
    21181 aaaaagagca tcagtttgcg tacggttgca gccgcactac tgctgcaaat tgctcttggt
    21241 ggcatcatgc tctattttcc gccgggaaag tgggctgtag aacaggcggc attaggcgtt
    21301 cataaagtga tgtcttacag tgatgctggt agcgccttca tttttggttc gctggtcggg
    21361 ccgaaaatgg atgtcctctt tgacggtgcg ggttttatct tcgcctttcg cgtacttccg
    21421 gcgattatct tcgtcactgc gctcattagt ctgctgtact acattggcgt gatggggctg
    21481 ctgattcgca tccttggcag cattttccag aaagccctca acatcagcaa aatcgaatct
    21541 tttgtcgcag tcaccactat tttcctcggg caaaatgaga tcccggcgat cgttaaaccg
    21601 tttatcgatc gcatgaatcg aaacgagttg tttacggcta tttgtagcgg gatggcgtcc
    21661 attgctggtt cgatgatgat tggttatgcc ggaatgggcg taccaattga ctacctgttg
    21721 gcggcatcgc tgatggcgat ccccggcggt attttgtttg cacgtattct tagtccagca
    21781 accgagcttt cgcaggtcac gtttgaaaat ctgtcgttca gcgaaacgcc gccaaaaagc
    21841 tttatcgaag cagcggcgag cggtgcgatg accgggctga aaatcgccgc tggtgtggcg
    21901 acggtggtaa tggcatttgt ggcaattatt gcgctgatca acggcattat cggcggaatt
    21961 ggcggctggt ttggcttcgc caatgcttct ctggaaagta tttttggcta tgtgctggca
    22021 ccgctggcgt ggatcatggg tgtggactgg agtgatgcca accttgcggg tagcctgatt
    22081 gggcagaaac tggcgattaa cgaattcgtc gcttacctga gtttctcccc atacctgcaa
    22141 acgggcggca cgctggaagt gaaaaccatt gcgattatct cctttgcgct ttgtggtttt
    22201 gctaactttg gttctatcgg tgttgtcgtt ggggcatttt cggctatttc gccaaaacgc
    22261 gcaccggaaa tcgctcagct tggtttacgg gcgctggcag cagcaacgct ttccaacctg
    22321 atgagcgcga ccattgccgg gttctttatt ggtctggcgt aatgctcaat ttgtagaccg
    22381 gataaaacgc atcaagcgtt gcatccggca atcgcgctat tgcaaataat ttttactgca
    22441 tcatcccgga gaatttattc tccgggtcca tctccagtgc cagccctgac aactgctttc
    22501 tgtttttaat tagatatcct ttcttgcttt taattaataa accgtcgtga atgaattgcg
    22561 cgagaacata taaaagatgt cgataagaaa cgcctaaata ctctgccgct tgcgtatgct
    22621 tttcgtgata aagatcacct tcttgtgaga gtaatataaa tgctgccagg cgattcacta
    22681 acggaaatga ttgattctga gttaaagaaa caatattacg ataattttta tgacttaagg
    22741 tgacgcaaag ttttcgtaaa aatagggtgt cgtttaataa cagcggacgg taatgtttca
    22801 tagggagcgc aaggcaccaa cactcttcaa tagcctgtac cgcacgcggt tcatggtctt
    22861 tatcgattaa ctcaatctca ccaataaaac agggggcggc aaagaaatcg atcagcgaca
    22921 cgcgaccatt agctagcgtg gcgtaaagcc tggcgcggcc tcgcgtcagg taaaacagcc
    22981 aggacggctg ttgcccttcc tgcacaatgt agtcacgcgc taaaaaatga aacagccgcg
    23041 tatcggctga aacatccgtt aaaaagcaat ccttaaacgc ggattcactc atcaattgct
    23101 gcttaagatc attattgtgg atttctttca cgatgtttat ccgctatgag atttctcata
    23161 tttagcatac gtacgctgtt gttaagattc aatcatcggc ccgatcgaaa tttctttgct
    23221 gtagaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga gaaaaattat
    23281 tctggattgt gatccgggtc atgatgatgc tattgctata atgatggcgg cgaaacatcc
    23341 ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg ataaaacatt
    23401 aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt atgcggggat
    23461 gccacaaccc attatgcggc aacaaatcgt tgccgataat attcacggtg ataccggact
    23521 ggatggcccg gtattcgagc cgctgacccg ccaggcagaa agcactcatg cggtgaaata
    23581 catcattgat accctgatgg caagcgatgg cgatatcact ctggtgccgg ttggtccgct
    23641 ttcaaatatc gcggtggcaa tgcgtatgca acccgcgatc ctgcccaaaa tccgtgaaat
    23701 tgtgctaatg ggcggcgctt acggtacagg caacttcacg ccatctgccg agttcaacat
    23761 ctttgctgac ccggaagccg cacgcgtggt gttcacctct ggcgttccat tagtgatgat
    23821 gggcctcgat ctcaccaacc agaccgtttg caccccggac gtgattgcac gaatggaaag
    23881 ggcaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaaccca
    23941 gttcgaaaac tacggcctgg ctggcggccc ggtgcacgac gccacctgca tcggttatct
    24001 gattaaccct gatggcatta aaacccagga gatgtacgtc gaagtggacg tcaacagtgg
    24061 cccttgttat gggcgtaccg tctgcgacga gctgggcgtt cttggcaagc ccgctaatac
    24121 caaagtcggc atcactattg atagcgactg gttctggggc ttagtcgaag agtgcgtgcg
    24181 cggctacatc aaaacccatt aagtgttgat aacaagccgg gccggtatcg caagatgcag
    24241 gcctggctat ttctgatggg cagaaagccg tcgcccaata tgacatgaag agataatcac
    24301 tatggatgtc atgagaagtg ttctgggaat ggtggtattg ctgacgattg cgtttttact
    24361 gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt
    24421 cgtgattggc ggcattatgc tttggttacc gccagggcgt tgggtcgctg aaaaagtcgc
    24481 ttttggtgtg cataaagtga tggcgtacag cgacgcgggt agcgcattta tcttcggttc
    24541 tctggtcgga ccgaaaatgg acaccttatt tgatggcgca ggatttatct ttggtttcag
    24601 ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact acatcggtgt
    24661 gatggggatt ttaattcgca ttctcggcgg tatattccag aaagcattaa atatcagcaa
    24721 gattgagtca ttcgtcgcgg tcactaccat tttcctcggg caaaacgaaa ttccggcgat
    24781 tgtgaagccc tttatcgatc ggctgaatcg caatgaatta tttacagcga tttgtagcgg
    24841 catggcctcg attgctggtt cgacaatgat tggttatgcc gcactgggcg tgcctgtgga
    24901 atatttgctg gcggcatcgt taatggcgat ccccgggggg atcttgtttg cccgcctgct
    24961 aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca ccgaaacacc
    25021 gccaaaaagc attattgaag ccgccgcgac aggggcaatg accgggctga aaatcgccgc
    25081 aggtgtggcg acagtagtga tggcattcgt cgccatcatt gcgttaatta acggtattat
    25141 cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca ttgtaggtta
    25201 tctgctggcc ccactggcgt gggtaatggg tgttgactgg agtgatgcga atcttgccgg
    25261 gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca atttctcacc
    25321 ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt ccttcgcgtt
    25381 gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc
    25441 gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg cggcgacact
    25501 ttctaacctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt agtgtgcgat
    25561 ttatgagccg gataagatgc ggtcaagcgt cgaatccggc aacattgtta caccattggc
    25621 actaatgaaa gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgt
    25681 gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt tccttccaga
    25741 aagctatata ccaggcccgc cataaaacca tcgtcagcac caaaactgtc tactgtcgta
    25801 tgcgctggtg cggtcagcaa aaattgttct ccatcctttt cgctgcaata aactgactca
    25861 tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaacgcatt cactgcagca
    25921 ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc cggtaaagtg
    25981 ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt gaattctgaa
    26041 acggtatcga caaacaccgg gatctcacca gcgagggtaa agacccattc cagcgcctcg
    26101 gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg
    26161 ttcaataatt gcggcgataa ctgttccagc agatgggtat cgttaatcgc cagcacggtt
    26221 tcatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg taaacgaacg
    26281 cagccggaga cattcacgcc ggcgcggcgc gtttcttcca ggagcatttc gccataaaag
    26341 tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag attgtgggcg
    26401 atgttgcgtc ccacgccgcc cgctgagcaa tgaattgtac cgggatgaga agccgattgc
    26461 gggtaacgga tatccgccat cccgcgaata tccatattga ttgtccccac cactacgcag
    26521 tattcctgct cggtgagaat gtaacctttg cctttaatcc ggcctttgcg cattaaatcc
    26581 ataatatgcg ccgcaacgcg cgaacggctg atttgcagca tgtccgcaat ttcgttctgc
    26641 tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt attcatcttc
    26701 aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct gcccagatat
    26761 ccgggttgat ggtttcgagg atcagcggaa tgccgtcgaa acggtcgtcc tgcatgatcc
    26821 agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg tcaacgcggc
    26881 tgccaaaggt gcttttcgca tcgttaaggt gcatcccgcg cagatactta aagccgacag
    26941 tacgggcaaa atccgcgaat gttttctcgc attcggctgg agtacgcaaa tcatacccgg
    27001 cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct tccacgccgt
    27061 cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga ccggcggtgt
    27121 tttctatcac cgctgtcaca ccttgagttt tatccagcgc aatgttgatg gattcggcaa
    27181 tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg tggaagttga
    27241 gcaaagaaag ccccagctgt tcgcaacgct gcatttcatc tataaaggca tcgcgcgatt
    27301 tttccagagc ttcagtgacc ggatgtccga ggttaatcag ataactgtcg tggggaagaa
    27361 tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg atggtttgcg
    27421 tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca aacgcggttg
    27481 cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca ctaacgtgcg
    27541 ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac ggtaatgata
    27601 gcgggttaac aggagcaaga tgtagtggtt tatgcgatga cgctttgaat cacatagtta
    27661 atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag cagaggtttc
    27721 gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc catcgccatt
    27781 gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg taacaggtgg
    27841 aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat agtgattttg
    27901 cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag caggatgagg
    27961 aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt ttccgcatcc
    28021 gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt agaaccaatg
    28081 tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat cgccgggtag
    28141 aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt tactttactg
    28201 gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca gatactgcta
    28261 ccggcaccga tcaaccagct ggtgtgctta tccagaccaa acactttctg ccccaggaag
    28321 caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc actgataccg
    28381 acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc cagacgtaat
    28441 aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg cggatagatg
    28501 gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa cccggcaccc
    28561 gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc actcagggct
    28621 aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat attcgtcata
    28681 accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata aaattgatta
    28741 tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc tccggcagtt
    28801 ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caagcgtcgg tgatgctggc
    28861 gttgtcgcaa tcagcagtga gcgcagcctt gaccgacctg gaagggcagc ttggcgtgca
    28921 actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc tgctctatcc
    28981 gcgtgcgttg gcattgcttg aacaggcggt tgaaatcgaa caactgtttc gcgaagacaa
    29041 cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc ctgcagttat
    29101 cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg ggaatagcca
    29161 ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg aaggaccgtg
    29221 ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagctggtgg ttttcgccgc
    29281 gccgacttcg ccgttggccc gtggtccggt caccttagaa cagctggccg ctgcgccgtg
    29341 gatcctgcgt gaacgcggtt ccggcacgcg ggagattgtc gattatctgt tgctgtcaca
    29401 tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca aacatgcggt
    29461 gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc aattgcaggc
    29521 aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat
    29581 acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact attgcgatcc
    29641 cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt cgatttcgtg
    29701 acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac ttataatccc
    29761 tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt gccaatcgtc
    29821 tgctacaatc gcgcctcatt ttttagatgg atagcatttt tgtatggttt ccgaaactaa
    29881 aaccacagaa gcgccgggct tacgccgtga attaaaggcg cgtcacctga cgatgattgc
    29941 cattggcggt tccatcggta caggtctttt tgttgcctct ggcgcaacga tttctcaggc
    30001 aggtccgggc ggggcattgc tctcgtatat gttgattggc ctgatggttt acttcctgat
    30061 gaccagtctc ggtgaactgg ctgcatatat gccggtttcc ggttcgtttg ccacttacgg
    30121 tcagaactat gttgaagagg gctttggctt cgcgctgggc tggaactact ggtacaactg
    30181 ggcggtgact attgccgttg acctggttgc agctcagctg gtcatgagct ggtggttccc
    30241 ggatacaccg ggctggatct ggagtgcgtt gttcctcggc gttatcttcc tgctgaacta
    30301 catctctgtt cgcggctttg gtgaagcgga atactggttc tcactgatca aagtcacgac
    30361 agttattgtc tttatcatcg ttggcgtgct gatgattatc ggtatcttca aaggcgcgca
    30421 gcctgcgggc tggagcaact ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc
    30481 gatgatcggc gtagctatga ttgtcggctt ctctttccag ggaaccgagc tgatcggtat
    30541 tgctgcaggc gagtccgaag atccggggaa aaacattcca cgcgcggtac gtcaggtgtt
    30601 ctggcgaatc ctgttgttct atgtgttcgc gatcctgatt atcagcctga ttattccgta
    30661 caccgatccg agcctgctgc gtaacgatgt taaagacatc agcgttagtc cgttcaccct
    30721 ggtgttccag cacgcgggtc tgctctctgc tgcggcggtg atgaacgcag ttattctgac
    30781 ggcggtgctg tcagcgggta actccggtat gtatgcgtct actcgtatgc tgtacaccct
    30841 ggcgtgtgac ggtaaagcgc cgcgcatttt cgctaaactg tcgcgtggtg gcgtgccgcg
    30901 taatgcgctg tatgcgacga cggtgattgc cggtctgtgc ttcctgacct ccatgtttgg
    30961 caaccagacg gtatacctgt ggctgctgaa tacctccggg atgaccggtt ttatcgcctg
    31021 gctggggatt gccattagcc actatcgctt ccgtcgcggt tacgtattgc agggacacga
    31081 cattaacgat ctgccgtacc gttcaggttt cttcccattg gggccgatct tcgcattcat
    31141 tctgtgtctg attatcactt tgggccagaa ctacgaagcg ttcctgaaag ataccattga
    31201 ctggggcggc gtagcggcaa cgtatattgg tatcccgctg ttcctgatta tttggttcgg
    31261 ctacaagctg attaaaggaa cccacttcgt acgctacagc gaaatgaagt tcccgcagaa
    31321 cgataagaaa taagtttctc ccttccttgc taagccctct caaccgagag ggctttttca
    31381 attccatttc cctgacaaat catgcggata taaaatttaa catttggatt gataattgtt
    31441 atcgtttgca ttatcgttac gccgcaatca aaaaaggctg acaaatcaga ggctggtccg
    31501 gctttctggg atgatcacct gcatacaaat aagtccaccg cgatgctgcc gtacgcaagg
    31561 ggacgtgaag aagatgtgag cgataaccca ttttattttc gtagttacct catggagata
    31621 tggaatgttt aggttgaacc ctttcgtacg ggtcgggctg tgtttgtccg ctatttcttg
    31681 tgcatggcct gtgttagcgg tcgatgacga tggcgaaacg atggttgtca ctgcatcttc
    31741 cgttgaacaa aaccttaaag atgcacccgc cagtatcagc gtcattaccc aggaagacct
    31801 gcagcgaaaa ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg gcgtacaact
    31861 gacgaacgaa ggggataacc gtaagggcgt tagtattcgt ggtctggaca gcagctacac
    31921 cctgattctt gtcgacggta aacgcgttaa ctcccgcaat gccgtcttcc gccacaatga
    31981 tttcgatctg aactggatcc cggtcgattc catcgaacgt attgaagtgg tccgtggccc
    32041 gatgtcgtcg ctgtacggtt ccgatgcgct cggcggtgta gtgaatatca tcaccaaaaa
    32101 aatcggtcag aaatggtcgg gtaccgttac cgtcgatacc accattcagg aacatcgcga
    32161 tcgcggtgat acctataacg gtcagttctt taccagtgga ccattaattg atggtgtgct
    32221 gggaatgaaa gcttacggca gcctggcaaa acgtgaaaag gatgacccgc aaaactcaac
    32281 gaccaccgat accggagaaa cgccgcgtat tgaaggattc tccagccgca acggcaatgt
    32341 cgaatttgcc tggacaccga atcaaaatca cgattttact gccggatacg gtttcgaccg
    32401 tcaggatcgt gattccgact cgctggacaa aaaccgcctg gaacgccaga actactccgt
    32461 cagccataat gggcgttggg attacggcac cagcgaactg aaatactacg gtgagaaagt
    32521 cgagaacaaa aaccctggca acagcagccc gataacttcc gaaagcaata cggtcgacgg
    32581 caaatacacg ttgccgctga cggcgattaa tcagtttctc acggttggcg gtgaatggcg
    32641 tcacgacaaa cttagcgatg cggtgaacct gaccggggga accagctcca aaacgtctgc
    32701 cagccagtac gcgctgtttg tggaagatga atggcggatc ttcgagccgc tggcgctgac
    32761 gaccggcgtg cgtatggacg atcacgaaac ctacggtgaa cactggagtc cgcgtgccta
    32821 cctggtttat aacgccaccg acaccgtaac ggtgaaaggg ggctgggcga cggcatttaa
    32881 agcaccttct ctgttgcaac ttagccctga ctggacgagc aattcctgcc gtggcgcatg
    32941 taagattgtg ggtagcccgg atctgaaacc agaaaccagc gaaagttggg agctggggct
    33001 ttactacatg ggtgaagaag gctggctgga aggggttgaa tccagcgtta ccgttttccg
    33061 taacgatgtg aaagatcgta tcagcatcag ccgtacgtct gacgtcaatg ctgcaccggg
    33121 ctaccaaaac tttgttggtt ttacagcgaa cggtgtgccg gtatttagct actacaacgt
    33181 taacaaagca cgtattcagg gcgtggaaac cgagctgaaa attccgttca acgatgaatg
    33241 gaaactgtca ctcaactaca cctacaacga tggtcgtgat gtcagcaacg gcgaaaacaa
    33301 accgctatcc gatctgccgt tccataccgc taacggtacg ctggactgga aaccgctggc
    33361 gctggaagac tggtcattct atgtttctgg tcactatacc gggcagaaac gagccgacag
    33421 cgcgacggct aaaacaccgg gcggttatac catctggaat accggcgcgg cctggcaggt
    33481 gactaaagac gtcaaactgc gcgcaggcgt gctgaacctt ggcgacaagg atctcagtcg
    33541 tgacgactac agctataacg aagacggacg tcgttacttt atggcagtgg attatcgctt
    33601 ctgatgagaa gatgcccggc gaaccgggcg gactttcact tcagtaaata ctgcgcatgg
    33661 aagcgcaggt gatcctctat aaaagaggcg atgaagtagt aactgtgatc atatcccggc
    33721 tgaatacgca gcgtcatcgg ccatgctttc tggcgcgcgg cttctgcaag tactgcaggt
    33781 tgcagttggt cagcaagaaa ctgatcgtta tcaccctgat cgataagcgt cgggatcgca
    33841 tcctgcgcgt tactggcata catcagcgcg caactgtccc attccagcca tgcatttttg
    33901 tcctcaccta aatagcggct aaacgctttg attccccacg ggacgctgca cggattcaca
    33961 attggcgcaa aggccgaaac gctggtgtat ttgcccgggt ttttcagcgc cataatcagc
    34021 gcaccgtgac cacccattga atgaccgcta atagcgcagc ggtcgctgac attaaattgc
    34081 gactgaacca gcgccggtaa ttcatcacgc agataatcat acatccggta atgcgtcgcc
    34141 cacggcggtt gcgtggcatt aagataaaag cctgcgccct ggcctaaatc gtagccatcg
    34201 tcgttggcaa ccttttcgcc gcgcgggctg gtgtctggca taaccagtac aatccccagt
    34261 tccgccgcca cgcgctgggc acccgccttg gtggtgaagt tctcgtcatt gcaggttaat
    34321 ccggaaagcc agtacagcac tggtggcggg gtgtgatcac gagggggtgg gagaaagata
    34381 ctgaacgtca tcgggcagtt taaggtactg gagtcgtgtc gccagcgttg ctgccagcct
    34441 tcaaaacagc ggtgctcttc gagcatttcc atgcgtggct ccttgttgtg ttgtttgcaa
    34501 tttcttcatc ataccgttta ttcaatgtgc tgtgagtaac tttcacttcc gtatttgcat
    34561 aacgatgttt taacatctgc tgatgaaagg cagcggcaat tacaataatt atcgctgtga
    34621 atactggatt atgtgcgccg cctcacgcac aataatcagg ctgtaaatca gcttaataac
    34681 tttgccccca cgcagggcgg aggcgtcaca cctgcaggag aaatcataaa tgccatcact
    34741 cagtaaagaa gcggccctgg ttcatgaagc gttagttgcg cgaggactgg aaacaccgct
    34801 gcgcccgccc gtgcatgaaa tggataacga aacgcgcaaa agccttattg ctggtcatat
    34861 gaccgaaatc atgcagctgc tgaatctcga cctggctgat gacagtttga tggaaacgcc
    34921 gcatcgcatc gctaaaatgt atgtcgatga aattttctcc ggtctggatt acgccaactt
    34981 cccgaaaatc accctcattg aaaacaaaat gaaggtcgat gaaatggtca ccgtgcgcga
    35041 tatcactctg accagtacct gtgaacacca ttttgttacc atcgatggca aagcgacggt
    35101 ggcttatatc ccgaaagatt cggtgatcgg tctgtcaaaa attaaccgta ttgtgcagtt
    35161 ctttgcccag cgtccgcagg tgcaggaacg tctgacgcag caaattctca ttgcgctaca
    35221 aacgctgctg ggcaccaata acgtggctgt ctctatcgac gcggtgcatt attgcgtgaa
    35281 ggcgcgtggc atccgcgatg caaccagtgc cacgacaacg acctctcttg gtggattgtt
    35341 caaatccagt cagaatacgc gccacgagtt tctgcgcgct gtgcgtcatc acaactgatt
    35401 taaaggcagg aaccatggag cgcaacgtca cgctcgactt tgttcgcggc gtcgccattc
    35461 tggggatcct gctattaaac atcagcgcct ttgggctacc aaaggcggct tatcttaatc
    35521 ccgcctggta cggcgctatt acgccgcagg atgcatggac ctgggcattt ctcgatctca
    35581 tcggccaggt gaaattcctc acgctttttg cgctgctgtt tggtgctggc ctgcaaatgt
    35641 tgctgccccg tggcagacgc tggatccagt cgcggttaac gctgttagtc ttgctgggct
    35701 ttattcacgg tttattgttc tgggacggcg atattctgct ggcttacggg ctggtgggct
    35761 taatctgctg gcggctggtg cgcgatgcgc catcggtaaa aagccttttt aataccggcg
    35821 tcatgcttta tctggtgggg cttggcgttt tgctgttatt ggggttgatt tccgatagcc
    35881 agactagccg cgcctggacg ccggatgcat cggctatttt atatgaaaaa tactggaagc
    35941 ttcacggcgg cgttgaagcg atcagtaatc gtgccgatgg tgttgggaac agtttattgg
    36001 cactgggcgc acagtatggc tggcaactgg ctgggatgat gctcattggt gccgcattga
    36061 tgcgcagtgg ctggctgaaa gggcagttca gcttacgtca ctatcgtcgt actggttttg
    36121 tgctagtggc gattggggtg atcattaacc ttcctgccat cgccctgcaa tggcagctgg
    36181 actgggcgta tcgctggtgt gccttcttac ttcaaatgcc gcgggaactg agtgcgccgt
    36241 ttcaggcgat tggctatgcg tcgctgtttt atggcttctg gccgcaattg agccgcttta
    36301 agctggtgct ggcgatcgcc tgcgtcggac ggatggcgct gaccaactat ctattgcaaa
    36361 cgctgatttg taccacgctt ttttaccacc tcggtctgtt tatgcagttt gaccgtctgg
    36421 aattgctggc gtttgttatt ccggtatggc tggcgaatat tctcttctct gttatctggc
    36481 tgcgttactt ccgccagggg ccggtggaat ggctctggcg tcagttaact ttgcgtgctg
    36541 ccggaccggc aatatctaaa acatcaagat aacgatctgg atcacattcg ttaacaaaac
    36601 ggctgtaacc gtttccattg ctgtgactcg attcacgaag tcctgtattc agtgctgaca
    36661 aaatagccgg cagcaagcag tcatttactg caatctcata acaggtagtg aatatgatca
    36721 ccattcgtga tgtagcgcgt caggctggcg tctctgtggc aacggtttcc cgggtgctca
    36781 ataacagcac gctggtcagt gccgacacgc gtgaagcagt aatgaaagcc gtgagtgagc
    36841 tggattatcg gccaaacgcc aatgcccagg cgctggcaac tcaggttagc gacaccattg
    36901 gcgtggtggt gatggacgtt tctgatgcgt ttttcggcgc gctggtaaaa gcggtggatc
    36961 tggtcgctca gcagcatcag aaatacgtgc taatcggcaa tagctatcat gaagcggaaa
    37021 aagagcgtca cgccattgag gtgttaattc gccagcgttg taatgcgttg attgttcact
    37081 caaaagcatt gagtgacgat gaactggcgc aatttatgga taacattccc ggtatggtgt
    37141 taatcaaccg cgttgtgccg gggtacaccc atcgttgcgt ttgcctggat aatctcagcg
    37201 gtgcccgaat ggcgacgcgc atgttgctga ataacggtca tcaacgtatt ggttatcttt
    37261 cttccagcca cggcattgaa gatgacgcca tgcgtaaagc aggctggatg agtgcgttga
    37321 aagagcagga tattattccg ccggaaagct ggattggcac tggtacgccg gacatgccgg
    37381 gcggtgaggc ggcgatggtt gaactgctgg ggcgcaatct acaacttacc gctgtatttg
    37441 cttataacga caatatggct gctggcgcac tgacagcatt aaaagataat ggcattgcga
    37501 ttccgttaca tctctcaatc atcggtttcg atgatattcc catcgcccgt tacaccgacc
    37561 cgcaattaac gaccgtgcgt tatcccattg cttcaatggc gaaattagcc accgaactgg
    37621 ccttgcaggg ggcagcaggc aatattgatc ctcgtgccag ccactgtttt atgccgacgt
    37681 tagtgcgtcg ccattctgtc gcaacacgcc agaatgcggc ggcgatcact aactcaacaa
    37741 atcaggcgat gtaaccgctt tcaatctgtg agtgatttca cagtatctta acaatgtgat
    37801 agctatgatt gcaccgtttt aacgttgtaa cccgtatgta acagtgaata atcactttca
    37861 ccgaggtaat gcgtcataac aacaattaaa gccgttttct ggagcgttac cgggcatgga
    37921 agaacgaatt ttaaaaagtg agcttcggcg ttcagtaaca cttcattaac tctactgccc
    37981 cgccgagcat ttatctcaag cactaccctg cataagaaaa accggagata ccatgaataa
    38041 gaaggtgtta accctgtctg ctgtgatggc cagcatgtta ttcggtgcca ctgcacacgc
    38101 tgctgatact cgcattggtg taacaatcta taagtacgac gataacttta tgtctgtagt
    38161 gcgcaaggct attgagcaag atgcgaaagc cgcgccagat gttcagctgc tgatgaatga
    38221 ttcacagaat gaccagtcca agcagaacga tcagatcgac gtattgctgg cgaaaggggt
    38281 gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc
    38341 gcgtgggcaa aatgtgccgg tggtattctt taacaaagaa ccgtctcgta aggcgctgga
    38401 tagctacgac aaagcctact acgttggcac tgactccaaa gagtccggca ttattcaagg
    38461 cgatttgatt gctaaacact gggcggcgaa tcagggttgg gatctgaata aagacggtca
    38521 gattcaattc gtactgctga aaggtgaacc gggccatccg gatgcagaag cacgtaccac
    38581 ttacgtgatt aaagagctga acgacaaagg cattaaaact gaacagttac agttagatac
    38641 cgctatgtgg gataccgctc aggcgaaaga taagatggac gcctggctgt ctggcccgaa
    38701 cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga
    38761 agcactgaaa gcacacaaca agtccagcat tccggtgttt ggcgtcgatg ctctgccaga
    38821 agcgctggcg ctggtgaaat ccggtgcact ggcgggcacc gtactgaacg atgctaacaa
    38881 ccaggcgaaa gcgacctttg atctggcgaa aaacctggcc gatggtaaag gtgcggctga
    38941 tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta ccttatgttg gcgtagataa
    39001 agacaacctg gctgaattca gcaagaaata agactgattg ggcgcagtct attactgcgc
    39061 ccttattgta tggccaacaa ggtaaaatta tatggtcagc tcaacgactc cgtcttccgg
    39121 ggaatacttg ttggaaatga gcggtatcaa caagtctttt cctggtgtta aggcacttga
    39181 taacgttaat ttaaaagtcc ggccacattc tatccatgca ttaatggggg aaaacggcgc
    39241 aggaaaatca acattattaa aatgcctgtt tggtatttat aaaaaagact ccggcaccat
    39301 tttattccag ggtaaagaga tcgatttcca ttcggccaaa gaagcactgg aaaatggtat
    39361 ttcgatggta caccaggagt taaacctggt attacaacgt tcggtgatgg acaacatgtg
    39421 gcttgggcga taccccacca aaggcatgtt tgtcgatcag gacaaaatgt accgcgaaac
    39481 caaagcgatt tttgatgaac tggatattga tatcgatccg cgtgcgcgcg tcggcacatt
    39541 atctgtttcg caaatgcaga tgatcgaaat cgccaaagcg ttttcctata acgcgaaaat
    39601 tgtgattatg gatgaaccga cttcttcgtt aaccgaaaaa gaggtcaatc atctgttcac
    39661 tattattcgt aaattaaaag agcgcggctg cggtattgtt tatatctcgc ataaaatgga
    39721 ggaaatcttc cagttatgtg atgaagttac cgtattgcgc gacggtcagt ggatcgccac
    39781 cgaaccgctg gcagggctga cgatggacaa gattatcgcc atgatggttg ggcgttctct
    39841 taaccagcgt tttcctgaca aagaaaacaa gccgggcgaa gtcatcctcg aggtacgtaa
    39901 cctgacgtca ctgcgccagc cgtcgattcg cgatgtctcg tttgatctgc ataaagggga
    39961 gatcctcggt attgccggtc tggtgggggc gaaacgtacc gatattgttg agacgttatt
    40021 tggtattcgc gagaaatcgg ctggcaccat tacgttgcac ggcaaaaaga tcaataacca
    40081 taatgccaac gaagccataa accacggatt tgcactggta actgaggagc gccgctcaac
    40141 gggaatttat gcctatctgg atattggttt taactcgtta atttccaata ttcgcaacta
    40201 caaaaataaa gttggtttac tggataactc gcggatgaaa agcgataccc agtgggtgat
    40261 tgattcgatg cgggtaaaaa caccggggca tcggacgcaa attggttcgc tctcaggtgg
    40321 taatcagcaa aaggtgatta ttgggcgctg gctgttaacg caaccagaaa tattaatgct
    40381 cgatgaaccg acgcgcggta ttgatgttgg ggcgaagttt gagatttatc agttaattgc
    40441 cgaactggcg aagaaaggca aggggattat tattatctcc tctgaaatgc cagagttgtt
    40501 agggataaca gaccgtattc tggttatgag caatggcctc gtttccggaa ttgtcgatac
    40561 aaaaacaaca acgcaaaacg aaattctacg tcttgcgtct ttgcaccttt aagattaggg
    40621 gcttcccatg agtgcgttaa ataagaaaag ttttcttact tacctgaaag agggcggtat
    40681 ttacgtcgtt cttttagttt tgcttgcgat tattattttc caggccccaa catttttaag
    40741 tctgttgaac ttaagtaata ttctcaccca gtcatcggtg cgtattatta tcgcgctcgg
    40801 tgtggcaggg ttaattgtca cccaggggac cgacctttct gcggggcgtc aagtagggct
    40861 ggcggcagtg gtggcagcga cattattgca gtccatggat aacgccaaca aagtgttccc
    40921 ggaaatggcg acgatgccga ttgcgctggt tattctgatt gtctgtgcca ttggtgcggt
    40981 gatcggtttg atcaacggtc tgattatcgc ttatctcaac gtgacgccgt tcattaccac
    41041 gctcggcacg atgatcatcg tctatggcat caactcgctt tattacgact ttgtcggggc
    41101 gtcgccaatt tctggttttg acagtggctt ctctaccttt gctcagggct ttgtcgcgct
    41161 ggggagtttc cgtctctctt acatcacctt ctacgcgttg attgcggtgg cgttcgtctg
    41221 ggtgttgtgg aacaaaaccc gcttcggtaa gaacattttt gccattggcg gtaacccgga
    41281 agcggcaaaa gtatctggtg tcaacgtcgg cctgaacctg ctgatgatct acgcgttgtc
    41341 tggcgtgttc tatgcctttg gcgggatgtt agaagccgga cgtatcggct ctgccaccaa
    41401 caacctcggc tttatgtatg aactggatgc tatcgcggcg tgcgtggtag gcggtgtatc
    41461 gttcagcggc ggtgtgggga cggtgattgg cgtggtgacc ggggtaatta tttttaccgt
    41521 catcaactat ggcctgacgt acatcggcgt aaacccatac tggcagtaca tcatcaaagg
    41581 ggcgattatt atcttcgccg tagcgctgga ttcactgaag tacgcgcgta agaagtgaaa
    41641 tgattgtggt ccgcattcgg tcataaacgc acataacatc atgatctgtg tggttttgta
    41701 ggccggataa ggcgttcacg ccgcatccgg cgacaggagc caaatgccgg atgcaacgct
    41761 ggcgcgtttt atccggcctg caatgagctc aacatactga tttgtatggt tttgtaggcc
    41821 ggataaggcg ttcaagccgc atccggcgac aggaaccaaa tgccggatgc gtctctggtt
    41881 ttacaacgtt accgggtgtt ctttctcgcc tttcttaaac ttcacttccc cgagctcaat
    41941 acaacccacc gggcagacgt gaccacaaag cagacaaccc acacatttct cggtattaca
    42001 atgcggtgtg cgggttttct cgctccattc catcgcctgg tgaccgccgt cgtaacagga
    42061 aatataacag cgtccacagc caacacattt atcaagattg atacggggat agacaatata
    42121 actgcggtct aaatcttccg ccgggacaat attgttattc gccagaccta ccatctcctg
    42181 caacgaatca aatccctgat cggcgagata atgcgataac ccgctcgcca tatcttctac
    42241 gatgcgatag ccgtactgca taatgccggt ggtgacctgt aacgttgctg cgccgagcaa
    42301 taaaaactcc gccgcatcct cccaggtttc aatgccgccg ataccgctga ttgggaaatc
    42361 gcgcagttct ggatgggtgc gcatttgctg aatgaagcgc agggcgatcg gttttaccgc
    42421 tttaccggaa tatccggaaa tactcgattt tccgttaacg atcggcatac cgattttctg
    42481 attaagatcg atattggtga tggatttaac ggtgttaatc gccgcaatgc catctgcgcc
    42541 gccgcgcttc gccgccagcg ccacttcgca catatcaccg atattcggcg tcatcttcgc
    42601 cagcattggc agcgtggaac cccgtttcac tgcccgacaa tatttttcta ccagctccgg
    42661 gctttgcccg acatcgctac ccatcgcatg agaagtcatt tgcggacagg agaagttaca
    42721 ctcgatcata tccgcgccag cttcttgcac caggcgcgcc agctcctccc attgctgctc
    42781 attttccccc atgatcgaag cgatcaatac tttgtccggg taatcttcct tcagccgacg
    42841 cagggcggcc agattctctt ccaacggatg ttcagcaatc tgttccatat ttttgaagcc
    42901 gataaaaccg gtatcttctt tcaccagatg atcaaaacgc ggcgagactt cgttggcgat
    42961 aaaaaagccg atcgttttaa acaccacgcc gccccaacct gtgtcgtagg ctttggcaca
    43021 catctcatag cagttgccta ccggcgaaga agagaggcag aacgggttgg gaaacttcac
    43081 gccgcaaaaa gtaatcgaaa gatctttcgt taacatgagc aagctccctc taaatagtga
    43141 tgaatcgcct cggcggcttc tttcccggtt ttcacggcat agaccacggt tttgtcaccc
    43201 tcaacaatat cgccagcagc aaagacttgc ggatcgcggg tctgataatt ttgtgttttg
    43261 atggtgttac gctgcggctc taacttcgca aagtcatcca gtctggcatg ctgaccgacg
    43321 gcgagaataa ttttatctgc cgccatcgtc agttcgcccg ataaccgtac atgcttaaac
    43381 gtgactttat tgccttccac ggctactggc gtgaatccat cgatgatcga aacgcccagc
    43441 tcccgggcgc tggcaaactc tttttcgctt gccggaaact catctaactc ttcacgcgct
    43501 acgcaagtta ccgcctgaca gccgagaact ttcagcgtgc tggctacgtc catcgcgaca
    43561 tcaccgccgc cgataattaa tgcgctttgt ggaatgctga tatcgccttg cgcctgtcgt
    43621 gcacgtagca ggaagtcgac ggcaatctca acgtcgctat gctcaaacag cggtagcccg
    43681 gaaccgcttg ataaccccac ggtgaccagt accgcgcggt tttccgcttt cagttgctcc
    43741 agggtgagtg ttttaccgat ttcgttattg cacttaatgg tcacgcccat tttttcaata
    43801 cgggcgattt ctgcatccag cactgattgc ggtaaccgaa attgcggaat accgttacgc
    43861 aaccagccac cggggtgcgc ttctttctca taaatcgtga cgtcataacc ctggtttgtt
    43921 agcgtcacac tggcctgcaa tccggcagga cctgcgccaa taatcgcgac tttgccgagc
    43981 gttttagtac cgggctgata aatttccatc ccggtttgtt gttcaaaatc agtaacaaaa
    44041 cgctgtaagc ggccgatatc aatgggagca tcaacaccgg cacgggtaca accgctttgg
    44101 cataatttct ccgtcgggca cactctggca caaacggcac cgagggcatt attttcgcga
    44161 attgtctcgg cagcgccttt aaaattacga aagtagattg agcgaataaa tttccccgga
    44221 tcggtctgtg ccgggcaagc ctgactacag ggagcgtcgt gacataataa acagcgagag
    44281 gcttctttaa tcgccagtaa cggcgtaaaa gccggagtga gttcatccag ataattttgt
    44341 tgcggcatgt gatcttcctt aatgctatga gtaattaagg cgaacgctaa ataaatattg
    44401 cgtgctgata ttaaggctat gcgcgtgggg aaatttaaac tgtgaaagga ttcacaaata
    44461 gctgcggttc agaatgttat atccgcagct tattgagtga agtaataatt a
//
LOCUS       DAZKFZ010000013        43269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000013 DAZKFZ010000000
VERSION     DAZKFZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            425..1642
                     /gene="alaA"
                     /locus_tag="ACHOQW_000701"
     CDS             425..1642
                     /gene="alaA"
                     /locus_tag="ACHOQW_000701"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP9571828.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1726..2325
                     /gene="yfbR"
                     /locus_tag="ACHOQW_000702"
     CDS             1726..2325
                     /gene="yfbR"
                     /locus_tag="ACHOQW_000702"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9571829.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2384..4216)
                     /locus_tag="ACHOQW_000703"
     CDS             complement(2384..4216)
                     /locus_tag="ACHOQW_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP9571830.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4303..4953)
                     /gene="hxpA"
                     /locus_tag="ACHOQW_000704"
     CDS             complement(4303..4953)
                     /gene="hxpA"
                     /locus_tag="ACHOQW_000704"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP9571831.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4964..5458)
                     /locus_tag="ACHOQW_000705"
     CDS             complement(4964..5458)
                     /locus_tag="ACHOQW_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9571832.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5541..5996)
                     /gene="yfbV"
                     /locus_tag="ACHOQW_000706"
     CDS             complement(5541..5996)
                     /gene="yfbV"
                     /locus_tag="ACHOQW_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP9571833.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6334..7536
                     /gene="ackA"
                     /locus_tag="ACHOQW_000707"
     CDS             6334..7536
                     /gene="ackA"
                     /locus_tag="ACHOQW_000707"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP9571834.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7611..9755
                     /gene="pta"
                     /locus_tag="ACHOQW_000708"
     CDS             7611..9755
                     /gene="pta"
                     /locus_tag="ACHOQW_000708"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9571835.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9945..11465
                     /gene="yfcC"
                     /locus_tag="ACHOQW_000709"
     CDS             9945..11465
                     /gene="yfcC"
                     /locus_tag="ACHOQW_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9571836.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11498..12040)
                     /gene="yfcD"
                     /locus_tag="ACHOQW_000710"
     CDS             complement(11498..12040)
                     /gene="yfcD"
                     /locus_tag="ACHOQW_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP9571837.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12098..12649)
                     /gene="yfcE"
                     /locus_tag="ACHOQW_000711"
     CDS             complement(12098..12649)
                     /gene="yfcE"
                     /locus_tag="ACHOQW_000711"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP9571838.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12705..13349)
                     /gene="yfcF"
                     /locus_tag="ACHOQW_000712"
     CDS             complement(12705..13349)
                     /gene="yfcF"
                     /locus_tag="ACHOQW_000712"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP9571839.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13485..14132
                     /gene="yfcG"
                     /locus_tag="ACHOQW_000713"
     CDS             13485..14132
                     /gene="yfcG"
                     /locus_tag="ACHOQW_000713"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP9571840.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14189..14551
                     /gene="folX"
                     /locus_tag="ACHOQW_000714"
     CDS             14189..14551
                     /gene="folX"
                     /locus_tag="ACHOQW_000714"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP9571841.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14572..15465
                     /locus_tag="ACHOQW_000715"
     CDS             14572..15465
                     /locus_tag="ACHOQW_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP9571842.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15513..16403)
                     /gene="rpnB"
                     /locus_tag="ACHOQW_000716"
     CDS             complement(15513..16403)
                     /gene="rpnB"
                     /locus_tag="ACHOQW_000716"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP9571843.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16600..17373)
                     /gene="hisP"
                     /locus_tag="ACHOQW_000717"
     CDS             complement(16600..17373)
                     /gene="hisP"
                     /locus_tag="ACHOQW_000717"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP9571844.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17381..18097)
                     /locus_tag="ACHOQW_000718"
     CDS             complement(17381..18097)
                     /locus_tag="ACHOQW_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9571845.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18094..18780)
                     /gene="hisQ"
                     /locus_tag="ACHOQW_000719"
     CDS             complement(18094..18780)
                     /gene="hisQ"
                     /locus_tag="ACHOQW_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP9571846.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18870..19652)
                     /gene="hisJ"
                     /locus_tag="ACHOQW_000720"
     CDS             complement(18870..19652)
                     /gene="hisJ"
                     /locus_tag="ACHOQW_000720"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP9571847.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19873..20655)
                     /gene="argT"
                     /locus_tag="ACHOQW_000721"
     CDS             complement(19873..20655)
                     /gene="argT"
                     /locus_tag="ACHOQW_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP9571848.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20921..21490)
                     /gene="ubiX"
                     /locus_tag="ACHOQW_000722"
     CDS             complement(20921..21490)
                     /gene="ubiX"
                     /locus_tag="ACHOQW_000722"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP9571849.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21585..23102)
                     /gene="purF"
                     /locus_tag="ACHOQW_000723"
     CDS             complement(21585..23102)
                     /gene="purF"
                     /locus_tag="ACHOQW_000723"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9571850.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23139..23627)
                     /gene="cvpA"
                     /locus_tag="ACHOQW_000724"
     CDS             complement(23139..23627)
                     /gene="cvpA"
                     /locus_tag="ACHOQW_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP9571851.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(24159..24821)
                     /gene="dedD"
                     /locus_tag="ACHOQW_000725"
     CDS             complement(24159..24821)
                     /gene="dedD"
                     /locus_tag="ACHOQW_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP9571852.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24811..26079)
                     /gene="folC"
                     /locus_tag="ACHOQW_000726"
     CDS             complement(24811..26079)
                     /gene="folC"
                     /locus_tag="ACHOQW_000726"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP9571853.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(26149..27063)
                     /gene="accD"
                     /locus_tag="ACHOQW_000727"
     CDS             complement(26149..27063)
                     /gene="accD"
                     /locus_tag="ACHOQW_000727"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9571854.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27219..27878)
                     /locus_tag="ACHOQW_000728"
     CDS             complement(27219..27878)
                     /locus_tag="ACHOQW_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9571855.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27961..28773)
                     /gene="truA"
                     /locus_tag="ACHOQW_000729"
     CDS             complement(27961..28773)
                     /gene="truA"
                     /locus_tag="ACHOQW_000729"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9571856.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28773..29786)
                     /locus_tag="ACHOQW_000730"
     CDS             complement(28773..29786)
                     /locus_tag="ACHOQW_000730"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9571857.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29852..30988)
                     /gene="pdxB"
                     /locus_tag="ACHOQW_000731"
     CDS             complement(29852..30988)
                     /gene="pdxB"
                     /locus_tag="ACHOQW_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP9571858.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            31087..32082
                     /gene="flk"
                     /locus_tag="ACHOQW_000732"
     CDS             31087..32082
                     /gene="flk"
                     /locus_tag="ACHOQW_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP9571859.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(32079..33257)
                     /locus_tag="ACHOQW_000733"
     CDS             complement(32079..33257)
                     /locus_tag="ACHOQW_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9571860.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33532..34752)
                     /gene="fabB"
                     /locus_tag="ACHOQW_000734"
     CDS             complement(33532..34752)
                     /gene="fabB"
                     /locus_tag="ACHOQW_000734"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP9571861.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34911..36917
                     /gene="mnmC"
                     /locus_tag="ACHOQW_000735"
     CDS             34911..36917
                     /gene="mnmC"
                     /locus_tag="ACHOQW_000735"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP9571862.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(37038..37316)
                     /locus_tag="ACHOQW_000736"
     CDS             complement(37038..37316)
                     /locus_tag="ACHOQW_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP9571863.1"
                     /translation="MIAEFESRILELIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37350..37898)
                     /gene="epmC"
                     /locus_tag="ACHOQW_000737"
     CDS             complement(37350..37898)
                     /gene="epmC"
                     /locus_tag="ACHOQW_000737"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP9571864.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37898..38707)
                     /locus_tag="ACHOQW_000738"
     CDS             complement(37898..38707)
                     /locus_tag="ACHOQW_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9571865.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38707..39531)
                     /gene="mepA"
                     /locus_tag="ACHOQW_000739"
     CDS             complement(38707..39531)
                     /gene="mepA"
                     /locus_tag="ACHOQW_000739"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP9571866.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39535..40620)
                     /gene="aroC"
                     /locus_tag="ACHOQW_000740"
     CDS             complement(39535..40620)
                     /gene="aroC"
                     /locus_tag="ACHOQW_000740"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9571867.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40655..41587)
                     /gene="prmB"
                     /locus_tag="ACHOQW_000741"
     CDS             complement(40655..41587)
                     /gene="prmB"
                     /locus_tag="ACHOQW_000741"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9571868.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            41753..42304
                     /gene="smrB"
                     /locus_tag="ACHOQW_000742"
     CDS             41753..42304
                     /gene="smrB"
                     /locus_tag="ACHOQW_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP9571869.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42475..>43269)
                     /locus_tag="ACHOQW_000743"
     CDS             complement(42475..>43269)
                     /locus_tag="ACHOQW_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP9571870.1"
                     /translation="SLETPKCYAGDKLMSASFSSTKIYYAMKNVTASGSLYFYVTVVT
                     PGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTDTSNCPGINTRVFVCAY
                     MTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSICPTIPVASFDISWSDNY
                     VSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAYCRAVSDLLTFTSYGCD
                     NAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYVLNEL"
BASE COUNT        10703 a        11696 c        10790 g        10080 t
ORIGIN      
        1 tattaaataa taattaatta gcatcatcct cattcattaa ttccgcttaa caatagtttc
       61 acaccttgcc cccattgcga cacctcggaa atcatcacgc tgttaaactc tccgtaatgc
      121 ggaaatatca tcatcgcgtt tcattagtga attcttaaat gaggcatttt cacacaatta
      181 tcttacagat aaaaaaacca gacttacaat taagaatcag aacaattcac tatataacat
      241 tgcatgtaaa gcatatgcac ctcattattt tgtcattatt aagttattaa cagcacaatc
      301 gagccttccc ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag
      361 tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag
      421 attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg
      481 tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa
      541 catcggcaac ccagccccgt tcggttttga cgcgccagat gaaatcctcg ttgacgtgat
      601 acgcaacctg cctaccgctc aagggtattg cgattccaaa ggtctttact ccgcgcgtaa
      661 agccatcatg cagcactatc aggctcgtgg catgcgtgat gttaccgtgg aagatattta
      721 catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg
      781 ggacgaaatg ttggttcctg caccagatta cccactatgg accgcggcgg tttcgctttc
      841 cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga
      901 tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc gttattatca acccaaataa
      961 cccaaccggc gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca
     1021 gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctctacg acgacgctga
     1081 gcatcactca attgcgccgc tggcacctga cctgctgacc attaccttta atggactgtc
     1141 gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa
     1201 acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa
     1261 cgttcctgcg caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt
     1321 tattacccct ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaat tgatcaacga
     1381 tattccgggc gtttcctgcg tgaaaccttg tggtgcgctg tacatgttcc cgaaaatcga
     1441 cgccaaacgc tttaacattc acgacgatca gaaaatggtg ctggatttcc tgttgcagga
     1501 aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat
     1561 tgtcacgcta ccgcgtgtcg atgatatcga gctgtcgttg agcaagttcg cgcgtttcct
     1621 ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagtgtt
     1681 atcccgccac aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat
     1741 ttttttgccc atctctcccg cctgaaactc attaaccgct ggccgctcat gcgcaacgtg
     1801 cggacggaaa atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca
     1861 gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg
     1921 atgtaccacg atgcctcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc
     1981 aattcgcaaa tcgctcagga gtacaaggct attgaaaaaa tcgctcagca aaaactggtc
     2041 gatatggttc cggaagagct gcgggatatc tttgcgccgt taattgacga gcatgcatat
     2101 agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt
     2161 ctggaagaac tcgcggcggg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg
     2221 acgcttgaag cgcgccgcag ccaggagatg gactacttca tggaagtatt tgttcccagc
     2281 ttccatcttt cgctcgatga gattagccag gattcaccgc tgtaagcagc tggagtctgc
     2341 gtcgcatcag gcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca
     2401 tcgggatcat caccacacaa accgccatca cgataatggt gaacggtacc cccagcttca
     2461 caaagtcact gaagctgtaa tttcccggac ctaaaaccaa tgtgttaaca ggtgaagaaa
     2521 ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac catcgcgaat ggataaggcg
     2581 acacccccat cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg
     2641 tattagagat aaatagccca ataaccgccg acaagacaaa caaacaaccc agcatcatat
     2701 gtggcccgta accaccgcca atgtccatca gccctttcac cgccagcgcg acacctcccg
     2761 ttttctgtaa tgccacagca aacggcatca tcccaacgat caaaataatg ctcggccagt
     2821 gaatggattt ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg
     2881 cgatgatagc ggcaacagga ttaggaattt catctgtcag cattaacgcc accattagca
     2941 ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt
     3001 ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc
     3061 agttacccac aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt
     3121 ccagcgccac gccattgcgc tttagcccca ccacattcag tccgtagcgg gtacgaaaac
     3181 caatttcgcg caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca
     3241 tgcccacatc aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca
     3301 attgctcact acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa
     3361 cgtcacgcgc gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc
     3421 gccagcgttc aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca
     3481 gccgttgacc aatcattggc gatccggggc gaatagccag acgtcgcgca cgcccggtca
     3541 gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg
     3601 tctgggtatc ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca
     3661 gcacgaccag gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca
     3721 gcaattcact gttgactacc aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa
     3781 tcagcccggc aaaacttaac ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg
     3841 caacgcttaa caccacggga ataaagatag cgacaacgcc ggttgaactc ataaacgcgc
     3901 caagccccgc gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcaa
     3961 ctttgaccag ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa
     4021 taataaacaa ggcggcaatc aggacaacgt taggatcaga aaagccagaa aatacttctg
     4081 ggactgtcag cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca
     4141 tacgcactct gcccgtcgca aacaagacga tggcaacggc cagtaatgaa agaacccaaa
     4201 tcaattcacc gttcacaact tatccttgtt aattgagggg gatgacttga ttctgccata
     4261 aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatc
     4321 gccatttggc tgtttggtca cagtaatttg ctccagactg tggaggacca aatcgacctc
     4381 attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg acatggcaac ccgccgccag
     4441 gccagaaagc acgccagcgg gagcatcttc caccaccaca cactcctgcg gcgcaagccc
     4501 cagcagctgc gcgcctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc
     4561 agcggttaca aacacctctg gtgcgggaag ccccgctatt ttatggcgcg ctcgcgctac
     4621 cggcatggag ccagaagtca caatggccca cggaatacct gctttattca aatgattgag
     4681 taaggcgatt gcccccggaa gcgcggtaat accttcggtt tccgtggcct cgatttgctc
     4741 caaacgcgta aactcggcgg caatatcagc ctcggatttg cccgccataa aatggcgcag
     4801 agaggtgatc gcctgtttac cgtgaatgaa agccagcacc tcttccggcg ctaacccatg
     4861 acgtctggcc cagttgctcc acgcccgttc taccgcaggc agggaatcca ccagcgttcc
     4921 atcaagatca aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt
     4981 gattaatttc gttggcgctc aaatggtact gacgcgggca ggcatgccac acattaagca
     5041 tgcgctgata tttttcccac attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa
     5101 aatgggtata gcgcccttcc acattaacca taaagcggac ataaccgagg taacgtgctt
     5161 cagtggcggc gtcaaagccg aggaaggtga cacgacgttc atcgatggat tgctggtcct
     5221 gcaaattaga ccaggaaaca tgcaacgcat gatacatctc cataatgtcg atgatagtgc
     5281 ggcaggtttc ttctttcagc tcgccaaact cgcgatccag ttcacgcatc tgtaatccat
     5341 aaccccgctc aatgattgtt tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca
     5401 tagtcatcat cttgtactgg ttagacaaaa taagacgttg cgcgttggtc atttccattg
     5461 ttgactcctg tatcactcta ctacggtgaa aaaaaagaag gctgagtatg ccttctttta
     5521 tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag ttgtttgaag
     5581 gcacgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac gggtgccaac
     5641 acctgtccag actcctgcaa tttaccgcga acttcataaa accagttgag gattgcaggg
     5701 ggtaatggcg tgacagaacg cttgcccagc caccacaatc cctgcatggg taaacttaag
     5761 gcgaacaggg cagtggcaac tgccggccca agctgaccgc ccagggcaat ctgccagcag
     5821 agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat cttgataacg
     5881 cgattttcga caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc
     5941 tgtccccggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt tgacatgctt
     6001 cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact cagcgggaca
     6061 acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca gcctgaaggc
     6121 ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat tggctgaaaa
     6181 ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg
     6241 catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc tccctgacgt
     6301 ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt actggttctg
     6361 aactgcggta gttcttcact gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac
     6421 ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg gaaaatggac
     6481 ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc gctcaacttt
     6541 atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac tgctatcggt
     6601 caccgtatcg tacacggcgg cgaaaagtat accagctccg tagtgatcga tgagtctgtt
     6661 attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc tcacctgatc
     6721 ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt tgctgtattt
     6781 gacaccgcgt tccaccagac tatgccggaa gagtcttacc tctacgccct gccgtacaac
     6841 ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta
     6901 acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat catcacctgc
     6961 cacctgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt tgacacctct
     7021 atgggcctga ccccgctgga aggtctggtc atgggtaccc gttctggtga tatcgatccg
     7081 gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat caacaaactg
     7141 ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg ccgctatgtt
     7201 gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc
     7261 ctggcgaaat acatcggtgc ctacactgcg ctgatggatg gtcgtctgga cgctgttgta
     7321 ttcaccggtg gtatcggtga aaatgccgcg atggtccgtg aactgtctct gggcaaactg
     7381 ggcgtgctgg gctttgaagt tgatcatgaa cgcaatctgg ctgcacgttt cggcaaatct
     7441 ggtttcatca acaaagaagg tacccgtcct gcggtggtta tcccaaccaa cgaagaactg
     7501 gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag ctcagctggc
     7561 ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc gtgtcccgta
     7621 ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga
     7681 tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc
     7741 gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac tcttccacca
     7801 cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc agcaatcaga
     7861 aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa gacgctgaag
     7921 tcgttctggt tgaaggtctg gtcccgacac gtaagcacca atttgcccag tctctgaact
     7981 acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca
     8041 ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa
     8101 acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat gaacagggtc
     8161 gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa gtaaacaatg
     8221 ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgct gtgccgtgga
     8281 gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat gcgaccatca
     8341 tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc gcacgcagca
     8401 ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc
     8461 ctgacgtgct ggttgccgct tgcctggctg ccatgaacgg cgtagaaatc ggtgccctgc
     8521 tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg
     8581 ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacttct ctgagcctgc
     8641 agagcttcaa cctggaagtt ccagttgacg atcacgagcg tatcgagaaa gttcaggaat
     8701 acgttgctaa ctacatcaac gctgactgga tcgattctct gactgccact tctgagcgca
     8761 gccgtcgtct gtctccgcca gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg
     8821 gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca gccgctatct
     8881 gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag atcaaccgtg
     8941 ttgcagcgtc tcagggtgta gaactgggtg caggcattga aatcgttgat ccagaagtgg
     9001 ttcgcgaaag ctatgttggt cgtctggtcg agctgcgtaa gaacaaaggc atgaccgaaa
     9061 ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg tacgctgatg ctggaacagg
     9121 atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc atccgtccgc
     9181 cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg ttcttcatgc
     9241 tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat ccgaccgcag
     9301 aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcagccttc ggtatcgaac
     9361 cgcgcgttgc tatgctctcc tactctaccg gtacttctgg tgcaggtagc gacgtagaaa
     9421 aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc
     9481 cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg ccgaactctc
     9541 cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt aacaccacct
     9601 acaaagcggt acagcgttct gccgacctga tctccatcgg gccgatgctg cagggtatgc
     9661 gcaagccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc tacaccatcg
     9721 cgctgactgc gattcagtct gcacagcagc agtaacctcg tcatcatccg cagctttgcg
     9781 ctgcggatat ctgaaccgga aatagtcact atttccggtt ttttattctc ttaatttgca
     9841 ttaatccttt ctgattatct tgcttaactg cgctgcatca atgaattgcg ccatctcact
     9901 ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg
     9961 aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg
    10021 ctattttaac cagccttgcc acctgggtag ttccggtggg gatgtttgac agtcaggaag
    10081 tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca cactcatttc
    10141 gccttctgac taacgaagca ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg
    10201 gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg gttaacctca ggatcgaaat
    10261 acgggacagc cgttggcatc atcatgttta tgctggtgat tggcggcgcg tttggcattg
    10321 tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat acccgtggga
    10381 atgaaattct ctttattcct gcgctgttta ttctgttttc acttggcggc gcggtatttg
    10441 gtatggggga agaggccgtc gcctttgcca ttatcatcgc accgctaatg gtccggctgg
    10501 gctatgacag tattaccacc gtcctggtga cctatattgc cacgcaaatc ggttttgcca
    10561 gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc
    10621 tttctggctc cgggttgcgc atcgtggtgt gggttatcgc cactctgatt ggcctgatct
    10681 ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt
    10741 ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt acctttggtg
    10801 actggctggt attgattgtc ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg
    10861 ttaatgcctg gtttattcca gaaattgcca gccagttctt caccatgggt ctggtgattg
    10921 gcatcatcgg cgttgttttc cgccttaacg gtatgacggt taataccatg gcttcatcct
    10981 tcaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc gccaaaggga
    11041 ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag cgtgttaaat actatcctca
    11101 acagcattgc caatgccatt agcggtctgg acaacgcggt cgcggcctgg tttatgttgc
    11161 tcttccaggc ggtatttaat ttcttcgtga cgtccggttc tggtcaggcg gcgttaacca
    11221 tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg
    11281 ctttccagtt tggtgatggc ttcagtcaca tcatttaccc gacctcagct tcgttaatgg
    11341 cgacgctcgg tgtttgccgg gtggacttcc gtaactggct gaaggtgggc gcgaccctgc
    11401 ttggactgct gtttattatg tccagcgtcg tggtgatcgg cgctcagttg atgggctacc
    11461 actaaaaatg ttaagagccg caattgcggc tcttttttca ttctgccgtt tcagtctcta
    11521 cggcttcatt tttggcattg cgcttcatcc acaacgctag cgctttcagc gagtctggag
    11581 tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact tcactgactt
    11641 catcttcctg tagggcgaag ggaccgtgag agacgcagct gaacaatgcg ccccagacac
    11701 ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca ccggcaatgc
    11761 ccaactcttc ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg gcctggacta
    11821 caccgccagc ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct
    11881 ggaccagaat tttgcccatg ccatcatgca cgacgatgta agttgcacga tgacgcagac
    11941 actgtgcccg catttgctcc cggctggctt gtgcaatgac ttcgttctct tcattgacaa
    12001 tatccaccca ttcagtactt gccaaacgac gctgttccat tatcaggaaa ccttcttttt
    12061 ctggcgctct tacggcgcat tttgagttgt gggtaaatta cggattaatc gcgacctgcg
    12121 caatgatact ttgatcattg agtgcgatta cactaagtac gtcattatcc agcatgccat
    12181 aactcgccgg attaccgcct ttcggaatac tcaccgagcc ggggttgaag tggaaaatct
    12241 ccccccgctg ttctgctact ggcagatggg tatgaccgta aaccagcaca tcgttctggt
    12301 ttaaagcagg cagattttcc gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt
    12361 tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa atgcagcagc atttgatcca
    12421 cttcgctgtc gcagttgccg cgcacagcga taaccttatg tgccacttca ttaagccgtt
    12481 cagcgacttt ggctggcgcg taaccctccg gtaaagcatt acgcgggccg tgattcaaca
    12541 cgtcgccaag gatcaccagc cactgggcac cgctttgggc aaacaactcc agaacacgtt
    12601 ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat cagtttcatc actcactcct
    12661 cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc agattgcttc
    12721 gctgaaagtg caataaaacg ctggacagac gctcgctgcc actggaatgt tgcataatcc
    12781 accaggcatt ctggcacctc atcgccatgt agtaccaggc ggttaatcat tagcgccaga
    12841 tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtac taacaaatgt
    12901 tctgccatcg cgaacagttt ctctgcactg gcttttccct cggccgttag tggcgctttt
    12961 ttcgcccccg caaagacaac atctgtcgga cgctcttcgc ggatgggcat cagatcgctg
    13021 cgcaaccagg cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag cggataaata
    13081 cgttcccagg tcggtggcgc aaatcgatct tccagatact ccgcaatggc agaagattca
    13141 ctcaactcaa aatcatcgat ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc
    13201 cacgtcggtt gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg aaacaacagg
    13261 cctttttcct gcaacgccac ccaagcggat aacacataag gggagaaaaa atgggcatct
    13321 gaccaaagcg tgatagcggg tttactcatt atgtcctcgg caataaaggg ggctggctat
    13381 caaactatag agcctttttg tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct
    13441 ttctataatt gtggtgttca gatgattaga caaccggagc tccaatgatc gatctctatt
    13501 tcgccccgac acccaatggc cacaaaatta cgctgtttct cgaagaagca gggctggatt
    13561 atcgcttgat aaaggtagac ctggggaaag gcggtcagtt tcgcccggaa tttttgctca
    13621 tttcgcctaa caacaaaatt ccggcaattg ttgatcattc tccagccgat ggcggcgaac
    13681 cgctaagcct ctttgaatct ggtgccattt tgttgtatct ggctgagaaa acggggctct
    13741 ttttgagtca tgaaacgcgt gaacgcgccg ccacattaca gtggttattc tggcaggtag
    13801 gcggactggg gccgatgctt gggcaaaatc atcattttaa tcacgcagcc ccccaaacca
    13861 ttccttacgc tattgaacgt tatcaggttg aaactcagcg tctgtaccat gtactgaaca
    13921 agcggctgga aaactcgccc tggctgggag gcgagaacta cagcattgcg gatattgcct
    13981 gctggccgtg ggttaatgcc tggactcgcc agcgaataga cctcgctatg tatccggcag
    14041 tcaagaactg gcatgagcgg atccgttcgc gccctgccac cgggcaggca ctgctaaaag
    14101 cacaactcgg tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg tattatttca
    14161 tcctaagtaa aacaacggag aacctgcaat ggcacaacct gccgctatta ttcgtataaa
    14221 gaaccttcgt ttacgtacat ttatcggaat taaggaagaa gaaattaaca accgtcagga
    14281 tattgttatc aatgtgacga tccactaccc cgccgataaa gcgcgcacta gcgaagatat
    14341 caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt attcagcatg tagagaataa
    14401 ccgtttctct ttgctggaaa aattaactca ggatgtgctc gatatcgcac gtgaacatca
    14461 ctgggtgacg tatgctgaag tggagatcga taaactgcac gcgctgcgct acgccgattc
    14521 ggtatccatg accttaagct ggcagcgtta accgccatat cgggaggctg catgaacata
    14581 gtgatcaccg gagggacggg attaattggt cgccatttga ttccacgttt gctggagctg
    14641 ggccatcaaa tcacggtaat gacgcgtaac ccgcagaaag ccagttccgt tctcggccct
    14701 cgggtgacac tatggcaagg gcttgccgat caaagcaacc tcaacggcat tgatgcggta
    14761 atcaacctgg ccggagaacc gattgctgat aaacgttgga ctcacgagca aaaagagcgt
    14821 ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg atttgattaa tgccagcgac
    14881 acgccaccgt cggtactcat ttccggctcg gcaacgggct attatggcga cttaggtgaa
    14941 gtggtggtta ccgaagagga accgccgcat aacaaattta cccataaact ctgcgcccgc
    15001 tgggaagaaa ttgcctgtcg ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgtacc
    15061 ggcgtagtgc tggcaccgga tggcggtatt ctcggtaaaa tgctgccgcc gtttcgtctt
    15121 ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg cctggattca tatcgatgat
    15181 atggtcaacg gcattctctg gctgctggat aacgagctgc gcgggccatt taatatggtt
    15241 tcgccctacc cgatacgcaa tgaacaattt gcccatgcgc tcggtcatgc gctgcatcgc
    15301 ccggctattt tgcgcgtccc cgcaaccgcc attcggctgt taatgggcga atcttcagta
    15361 ctggtattag gtggacaacg cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt
    15421 cgctggtacg atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg tttactgcaa
    15481 acatccgcca gttaactccc ggtgttacag ggttagtggc tttgcgcgat aagatcgtct
    15541 ggtgaaagtc gggtcagcgt caggatgact tcgtggtcaa atcctttttc cagcatctcc
    15601 tgagcaatac gcagagcttc ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg
    15661 tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc gttctgttat ctcaccaata
    15721 aatgcacgga aacgctgggc atctcccgtt tgtaatacat aattaaacag ggcttttagc
    15781 tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac
    15841 agatcgcgct gacgaatatg tttctgaatt aactccaaca gcgccatttt acgatgttgc
    15901 ataatctcgt catccggcac cacggtaata tccaccaacg gaaaagccga tgaatatatt
    15961 ttacgggcaa tcgcaggctc ggcaaattca tcaagccagc agagtgaata aggataagga
    16021 cttctgcaac catgataaaa cagcatcggg atcaccaatg gaagctcttt atagcccaca
    16081 tcaagatggt tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc cattaattct
    16141 tccggcttac tttggtgctc tatcactaca taaagataac ccactccctc ctgcgttttc
    16201 acagaccaca agaggtcgga ataatattgc cgcaggtctt catcaataaa actgtttggt
    16261 tccagtttaa gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag atgaatatca
    16321 ataaaatccc gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc atcatgcggc
    16381 gtggaagttg tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac
    16441 aataaccacg attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag
    16501 aagtattttt agcggactgc aacttttaca tttcagcgga agctgactcg caaattcccc
    16561 ttgaaaatac tccggcagcg ttagccaccg gagaggcaat tatttcagcg aacctttaag
    16621 gaactgttgc agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccttcttc
    16681 ctctattttc ccctgatgga ggaaaatgac gtgagtagat acatggcgag caaagcccat
    16741 ttcgtgggtc accaccacca tcgtttttcc ctcttccgcc agctgctgca taatgcgcag
    16801 cacttcaccc accagctctg ggtcaagggc cgatgtcggt tcatcaaaca acagaacttc
    16861 cggttccatt gccagcgccc gcgctatgga aacacgctgt tgctgaccac cggacaggtg
    16921 caccgggtat ttcccctgcg cacgttcgtc tatcccgact tttgccagat acttcaccgc
    16981 ccgctcgcgc gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt
    17041 ttccagcacc gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt
    17101 gcgcagtaag cgcagttgat ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac
    17161 cagattgatc gtctggccat tgaccacgat cgacccttca ctcggttttt cgaggaagtt
    17221 aatgcagcgc agaaaggtac ttttccccga tcccgacgat ccgatgatgc ttattacatc
    17281 tccggcattc gcttgcagtg acaccccttt cagcacttcg tgttcgccat agcgtttgtg
    17341 caaatcgata acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt
    17401 tcacatgctg caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga
    17461 tgattaaata gagcaccgcg gcaatgccga aggcggtaaa aggttgatac gtggcggcgt
    17521 taatatcgcg ggctattttc agcagatccg gcaccgtggc agtaaatgcc aacgcagtag
    17581 agtgcagcat caggatcact tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag
    17641 gcaaaataat gcagcgatac attttaaaag tcgagaagcc ataggctctg gcggcttcaa
    17701 tttccccatg cggcaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg
    17761 tgttaagcgt cagcgccagc acggtacagt tcaggccact gcggaaaaag gcgttaagaa
    17821 actcggtccc cttaacaatc tccagcgtat acatgccgga atagaacacc agcaactgaa
    17881 catacagcgg cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt
    17941 tattactgga gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg
    18001 acaaaataag cagccacaga gtgatcgcca caccagtaaa gcgataaccg tcggtccaca
    18061 gcagcggttt ccagtattca tgtaagattt cgatcacagg tcagccctct tcacacccac
    18121 agagtagcgg cgctcaagga acagcagcac accattggaa acggtggtga aaaccaggta
    18181 aatcacgcca cagacgatag cgaaatagaa cggttcccag gtacttttgc ctgccagctg
    18241 cgtggctttg accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat
    18301 cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat
    18361 ccgccgaaac acttgcccac gagtaaaacc gaacgccctc gccgcctcta tatgtccttt
    18421 cggcactgcc ataaaagcac cacggaacgt ttcggtaaaa taagcaccgt agataaaacc
    18481 gagagtgata ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc
    18541 cgtcaccgtg tttagcgcaa tctgcaaacc gtagaaaatc agcagcatca acactaagtc
    18601 cggcacgcca cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg
    18661 cgagagctta ccgccagcgc caattaaacc gatgattaca gcgagcacta cagagctgat
    18721 agccagctcc agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat
    18781 gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa
    18841 gggagcgggc tttctttcac cgatggcgat taaccaccat aaacatcaaa atcgaagtac
    18901 tttttcgcta atttctcgta agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt
    18961 gcctcgcgca gttcgttatc ttctttacgc aggcccatgc cggtccctac gccaaacagt
    19021 ttttcatctt taacagacgg gccaccgaat ttgtaatctt taccgacagg ttgtttgagg
    19081 aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg
    19141 tcagaataaa tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccaa
    19201 tgttcattac cgaacgtctc ctgggtagtg ccctgcaata cgcctacccg tttgcctttc
    19261 agcgactcga ctgtcggctg aatgtcagaa tttttcgcca ccaccagacg agaatcggca
    19321 gcgtacagtt tgtcggtgaa ggctatttct tgctgacgtt tttccgtaat ggaaagcgat
    19381 gacatgatgg cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcggattt
    19441 tcgacaaacg tacattgcgt attgatgcgt ttgcataatt cctttgccag atcgatatcg
    19501 aagccaacca gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta
    19561 ccgatgcgga tgttttgcgg aatcgcagca aatgccgcag ttgcgctgga gaaggccaga
    19621 accagagaga gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata
    19681 gggaatattt acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat
    19741 atcccgcccc gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc
    19801 accacgatag tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt
    19861 ccctgcgacg tatcagtcac cgtagacatt aaagtcaaaa tactttttcg ccatcttgtc
    19921 gtaggtgccg tcctgacgca gctcgccaag cgccttattg aaggcagccg tcagttcagc
    19981 atcatcttta cgtagcccta caccggtgcc atcaccgaag tattttttgt cttttactga
    20041 tgagccagca aaggcgaaat ctttaccagc aggttgcttg aggaatcctt cgctggcagc
    20101 aacttcatcc tgcagtgcag catccagacg tcctgcagcc agatcggaat agaccaaatc
    20161 ctggttggca taggccacca catccacacc tttgctacgc caggtctcgt tagcgtaagc
    20221 ttcctgggtt gatccctgca gcacaccaac atgtttacct ttcagtgagt ccagcgttgg
    20281 ctgaatcggc gaacctttgg cggcaatcag acgggaatct gcggcgtaca gcttgtcgga
    20341 gaaggcaatc tcctgctgac gtttatcggt aatggaaagc gacgaaataa tggcgtcgat
    20401 ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt
    20461 cacctgcatc cgtttgcaca tctcgttacc gagatcgata tcaaagccga caaagtcacc
    20521 tttggcatct ttcgacgaga acggtgcata ggtggtatcg gttccgatac gtaccgtctc
    20581 cggcaatgcc gcatagctgg aagccgctgc ggaaataccg actaacaaag acagagcgag
    20641 aatcgacttc ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg
    20701 ttgtgtgttt gcaggcttgt tcatgcaggt cttatgccat cttgacgaca acagtaacat
    20761 tcaacgttaa atatgttaat aagacgttgc attattgtcc taaagttgaa gatagcaggt
    20821 atagcggttg aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat
    20881 cgcggttgca aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg
    20941 caaagagatc ttcaggaagg gttatcgcaa actggtcaag aacacgatta accgtctgat
    21001 ttatcacatc atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca
    21061 ccgcaccgat ttctgctgcc tgcgtcatta aacgcagatg gcctaagtgc aatggtgttt
    21121 cacgcacgca gagcaccagt gggcgacgct ctttcagcac cacatctgcc gcacgggtca
    21181 gtaagccatc agtatagcta tggacaatgc cggacagggt tttgattgaa cagggtaaaa
    21241 tcaccatccc cagtgtctgg aaagaaccgg aagagatgct ggcggcaata tcgcgcgcat
    21301 catgcgtgac atcagctaat gcctgcactt cgcgcaggga aaaatccgtt tcgagggata
    21361 aggtctggcg cgccgcctgg ctcattacca gatgcgtttc gatatctgtg acatcgcgca
    21421 gaacctgtaa taagcgcaca ccataaatcg cgccgctggc accgctgatg cccacaataa
    21481 gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag actttgcagg atttcggcgg
    21541 gagttgcaag tcagggtgcc agaccggcac cctgagcgaa ggcatcatcc ttcgttatgc
    21601 atttcgagat tttccacttc gttctgacgt tgcactgctt tggcgtcgtc attacgtaaa
    21661 gtatcgagga aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac
    21721 accgagcatt caaactgctg gatatccggg ttttcagcgc gaacggcttc gatcagatcg
    21781 ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atctacttcg
    21841 cgaccgtgag cgatcagttc cgtggcgctc ggcatatcaa taccataaac gttcgggaag
    21901 cgaatttccg gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc
    21961 tcgataatct gctcagaagt ggtgccacgg acgatagagt cgtcgaccag caggacgttt
    22021 ttatcgcgga actcggcgcg gttggcgttc agtttacggc gcacggactt acgacgcagc
    22081 tgctgacccg gcatgatgaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg
    22141 tacggtttgc ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttccgggatc
    22201 gggatcacca cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagcttc
    22261 gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt cgatgaacga gtccgggcga
    22321 gcaaagtata catactcaaa caggcacgga ttgctgaccg gattgtcagc acattgacgg
    22381 gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc caggcgcgac gtcacgcagg
    22441 aaatcaaagc ccagtgtatc gagcgcaacg ctttcggaag cgaccatata ttctgtgcgg
    22501 ttctcgtcaa tatcacgttt tcccagaacc agcggacgaa taccgtttgg atcgcggaaa
    22561 gcaaccatac cgtggccgat aatcatcgcc acacaggcat acgcgccgcg gattaagcgg
    22621 tttgtagcag cgatggcggc gaaaatgttg tcggcttcaa gcgggtagtg gcggaagttg
    22681 tccagctcgc tggcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg
    22741 cggcgttttt cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg
    22801 tgggcaagcg taatgccata cggagagtta acgtaaaacg gctgcgcttc agaggcgctg
    22861 gagctgccag ccgtagggta acgcacatga ccaatgccca tattgccctg caaacgctgc
    22921 atatggcgag cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag
    22981 ttattggcat ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt
    23041 aaggcatcat aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac
    23101 atacgtcttt ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt
    23161 gacgagcttt gcagataatc aaaaaaccat ctgatgataa aactgaactg cggaatcagt
    23221 tgtgatttac tccagtcttc gcttttcgac accccggtaa aggagtcgag aaagaagaga
    23281 atagcagcaa caatcaacac accgcgcaat gcaccgaaac agacgcccag cacccgatcg
    23341 gtgcctgaca accccgtttt ctccaccagc aggcctatca cgaagttcac gatagcgcca
    23401 acgatcaggg tggcgataaa caggaccgcg atggcaatcc catttcgaac cagttcgtct
    23461 tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca
    23521 caaccccatg tcaccagcga taacgcttca cgaacaaagc cgcggatcag gctaaccaga
    23581 gaggaaaaag caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga
    23641 ttttacgccc tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta
    23701 gggatttcct tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac
    23761 tttttatgcc tttgcggcat cggacaatgc gtgtcggatg cggcgtaaac gtcttatccg
    23821 acctacggtt ctacccctgc gtaggcctga taagacgcac cagcgtcgca tcaggcaacg
    23881 gctgtcggat gcggcgtgaa cgccttatcc gacctacggt tctacccctg cgtaggcctg
    23941 ataagacgca ccagcgtcgc atcaggcaac gcctgtcgga tgcggcgtga atgccttatc
    24001 cgacctacgg ttctacccct gcgtaggcct gataagacgc gccagcgtcg catcaggcaa
    24061 cggctgtcgg atgcggcgta aacgccttat ccgacctacg gttctacccc tgcgtaggcc
    24121 tgataagacg cgccagcgtc gcatcaggca agaccgtatt aattcggcgt atagcccatt
    24181 accacgccac ttaagccaga aagttgcttc aactcaccca gcgaaccttt cagcttatct
    24241 ttcgaggcat ccggcccaac cagaatacgg gtaattttac cctgcactgg cgtggatggc
    24301 gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt cactttatcg
    24361 gcatttttca gcgcgcccag ttgcacaaca taggctttac ccgttggcgc tgctttttct
    24421 tccacgaccg gcttcggttc tggcttcggc gcaggtggtg cttcaacctt cggcttaggc
    24481 ggctccaccg gtttcggctt cggtgggacg accggtgcag gttccggttc aaactcggtg
    24541 ttattggctg caatagtggc cggatcgagc gacggtgctg cggcatcacc tgcccgcacc
    24601 tcttccgctg cgccttccgg cggttgcgtc ggcaacgcct gggtggcggc tggcatcata
    24661 tcaggctcat cgcgatcgcc cgcttttggc accagcggga tagccgcaaa ctcatcctga
    24721 taatgttttt tctgcccgtc cagcagccct ggaagtacaa tcacccccag cgccaccagc
    24781 acgatcgtgc ccactaaccg attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat
    24841 cacttccatg acatgtgcga ccgtgtggaa cgaaccacac accagcacgg tgtcttccgc
    24901 tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt
    24961 acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca
    25021 ataccagtca tcaaccacgc ttttcaacca ggccagagtt ccggcaatat ctttatcatg
    25081 tagcataccg ataaccgcca gcacgcgccc gtttttcggt agcgttttca tacgcccggt
    25141 gagatattcc gccgcatgtg gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct
    25201 cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc
    25261 actgacctcc agcccgctgg cacgcagtgc cgccagcgcc gttgcggcat tcggttgcgg
    25321 gacaagcggc aacggcaggt tttccagcgt gccgtgagca tcgctaaacg cccagtcatg
    25381 atcggtgacg gaatagttcc actcaacgcc ccgacgttgt aacagtgcac ctttttcctg
    25441 cgccacatca gcaatggtag aaggcatttc cggctcaccg acaattgccg gtttttcgct
    25501 gcgaaagatg cctgctttct cgcgaccaat actttcgcga tctggcccca gccagtcggt
    25561 atgatccagc gcaatactgg ttactaccgc gacatcggcg tcgacaatat tggttgcgtc
    25621 cagacgaccg cccagcccta cttccagaat caccacgtca agttgtgcct gtttgaacag
    25681 ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga
    25741 ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc aattcctgac cctgcacacg
    25801 tacgcgctcg gtataacaca ccagatgagg cgaactgtag acgcccactt tgtaccctgc
    25861 cgccatcaga atcgactcca gcgtacggca ggtggtgcct ttgccattcg tacccgcaac
    25921 ggtaaacaca aatggcgctg gtttcagaac gccaagacgc gccgcgacct ggctcacgcg
    25981 ctcaaggccg agatcgatag ttttactgtg caggttttcc agataagaaa gccacgaagc
    26041 cagaggcgac gcggcttgag gagtgcgttt gataatcatg gtatccgctg attcgttacg
    26101 gtgagaatag caaaagggca gagccagtgg ccctgccctt atcagttatc aggcctcagg
    26161 ttcctgatcc ggtaccgggg gtaccactac gccttcacgc ggcgcttcag gattcggcgc
    26221 tggcagattc atcaacttcg ccagaatgct cgccagtttc aggcgcattt ccggacgacg
    26281 gacgatcatg tcgatcgcgc ctttctcgat caggaattca ctgcgctgga atcctggcgg
    26341 cagtttttcg cgaacggtct gttcgataac acgcggaccg gcaaagccga ttaacgcttt
    26401 cggttcagcg atgttgagat cgcccagcat ggcgaaactt gcagaaacac cgcccatcgt
    26461 cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc
    26521 agaggttttc gccatctgca tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga
    26581 ggcggagaag cagatcagcg ggcagttatc ttccagcgcc tgctcaacgg cacgcacgaa
    26641 acgtgcaccc acaacagacc ccattgaacc gcccataaag gcgaactcga atgccgcagc
    26701 gacaaccggc attccataca gagtgccttt catcaccacc agcgcatctt tttcgccggt
    26761 ttctttctgc gcagatgcca gacggtcttt atacttcttg gagtcacgaa acttcagcac
    26821 atctttcggc tcaagctcgc tacccagctc cacaaggctt ccttcatcta acaggctatg
    26881 caggcgatta cgcgctgtca tacgcatgtg atggtcacac ttcggacaga cctcaagatt
    26941 acgttccagc tcagcgcggt ataaaacctg accgcagcta tcacacttag tccacacccc
    27001 ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt ttaattcgtt caatccagct
    27061 cattagggac ctttctgtct gaacctggtt cgatgccagt tttatctttg gggacgcata
    27121 atgccatttt tgcccccaac agaccatgaa tgttgcacat taaaacataa cagcccgaaa
    27181 ctttggataa aaaagtggtc gaaccgcgga gttacttttt attttgcggc gcgtgccgca
    27241 gcgcgtttgt gacggattat ttcgatgacg cccggcaaaa tagaaaccac aataatcccg
    27301 acgatcagca gcttaaggtt atcctgaacc atcgggattg taccgaagaa atagcctgcg
    27361 taggtaaaaa gcagtaccca caacagtgcg ccgatcacgt tataagcagc gaaatgacgg
    27421 tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg ttctgacgat cggcacaaaa
    27481 cgggcgagaa taatcgtttt gccgccatgt ttctcataaa actgatgggt tttatcgaga
    27541 taactgcgac ggaaaatttt cgaattggga ttactgaata acttttcacc gaacaagcgc
    27601 ccgatagtgt aattgaccgc gtcacccaca atcgcggcga tcagcatcag caccaccatc
    27661 atatggacgt tgagatcgtt ggtttccagt gatgccaacg ctccggcaac aaacagcaat
    27721 gaatcacccg gtaaaaacgg cgttaccacc agaccggttt cacagaacaa aattaagaac
    27781 aaaatggcat aaacccagac gccgtactcc gcgaccagct ctgccagatg cacgtcaatg
    27841 tgcaggataa aatcgatgag gaaataaatc aggtccatat ttgcctatgc cttgtactcg
    27901 tcatctttca ggctgtaact acgttggcta cagcctgaaa tgttccgagc attattctcg
    27961 ttagtccgcc agaaatagcg ggcccattgg cggttttgga agatcatacc ggtcagggta
    28021 atccaccgcg accagataca gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc
    28081 ctttgccgcc agcaactctg ctatccagct ctccggctgg ttgtgggcac cgacttccat
    28141 caggctgccg acaatattcc tgaccatatg atgtacaaag gcattcgctt tgatatcaac
    28201 caccacataa ggaccgtgac gcgtgacatt aatatgcata acgttgcgcc acggggttcg
    28261 ggactggcac tgcaccgcac ggaacgaggt gaaatcattc tcgcccagca agcactgcgc
    28321 agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag
    28381 taccgccggg cgcagccgat gattgtagat gatgtagcga taacggcgag ccgtggcgct
    28441 aaatcgggca tgaaaatcat caggtacagc tttaacccaa cgcacggcga tgtcaccagg
    28501 taaattcgca tttacgccca acgtccacgc agcgtctttg cgctgcgcgg tagtttcgaa
    28561 atgcacaacc tgcccggtac cgtgtacccc tgcgtcagta cgcccggcgc agaagacggt
    28621 gatgggttcg ttcgccacct gggagagcgc cttttccagc ttctcctgca cactgcggac
    28681 ttcattctgc cgttgccagc cgtaatactt actgccgtcg tactcaatgc ccagcgcaat
    28741 tttataaact ggcggttgtt gctggtcgga cattagtaca gatactcctg caccagtttc
    28801 tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag
    28861 aactggactt gctccggcat accgtagtca ttacgcacgc agccaacaga aagatgcggc
    28921 gtacccgaag catcacctac ctgagttggg aattcgttct cttcagagag cacaatatct
    28981 tccccttgaa caaacgcatc acgcgcttct tctgctgcca gtggacgcag agcttcaaag
    29041 ttgaccatct gggcatgacc gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc
    29101 agtccttcgt cctgcaggat tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg
    29161 ctaccttcgc tatccggcag taacggcagc atgttgaacg cgagctgacg cccgaagaaa
    29221 tcttcttcgt caatcggaat gccgttgagc aatttcgcac tctgccccgc taacgcatcg
    29281 accgcttttt tgccctgggc ggaggctgaa atcaggctgg taacactgat acgcgacaaa
    29341 ccgccctgat cgattaacgg tttcagtgcc gccagcagct ggctggtcag actgtctggt
    29401 acggcgatga cattccggtt gcggtaatcg gtcagtacaa acgggtttac ttccggcacc
    29461 accagcggta cgtcgggttc aagagcaaac aatccgctgc tgtcgatcac caggcaacct
    29521 gagttggtcg cttcttcaac ccaggctgcg gtagcttctt tgcctgcgac aaaaaatgcc
    29581 agctgcgcct gcgtccagtc gaattcagca gcatcctgca cggtgattgt cttaccacca
    29641 aagcgcaatt gttcgcctgc gctttcgtta cgtgccagtg cataaatttc cccaaccggg
    29701 aactgacgtt cagccagcgt ttcaagcagg gcttcgccca cagcgccagt tgtgcccagg
    29761 acggcaatgt tccagccttc agacatggtg gtttactcca gaaatagcaa agctccctgc
    29821 caatgttata gcagagagca tgaagaagag attaacgtgc cggatgatga acggcgttaa
    29881 aacccagttt acacagcaat gatgccgcac tggcgtcatc acaaattaca tacagagatg
    29941 accattcacg gcgctcaaga tagtttttgc gcagtttatc gaactcaccc ggtatcccgg
    30001 cgactttacg cagcggtgcg tcatcgcggc gcacatcata caccaaatgc accagccttt
    30061 tcagcgtcgg ttgatcgagc ggtccatgca gagtaatgcg accaaactct ggcgcaggca
    30121 gtaatgtatc cagcgcaacg tgctgttcat gcccaataaa cttgctatag gcttcaaaca
    30181 cttgcgtagt accgcgtgct ttaccttcca gggtatagcc tgcaatatgc ggcgtgccga
    30241 tatccacttt tgtcagcaat tccacattta gttccggttc gccttcccag acatccagca
    30301 ctacgcttaa cttctggcct tcattcaggc aggtcagcag cgcggtatta tcgacgactg
    30361 cgccacggca ggcgttaatc agaatcgctc cgggcttcag gctacggatc agtttttcat
    30421 ccgccagatg cagcgttttg tacggcccgt ctttgaagag tggcgtatgg aaagtcagaa
    30481 tatccgcgcg ctggactaac tcatccagcg agcggaaatc accctcatcc ccacagtcgg
    30541 cgcgaggcgg atcgcaaagt aaggttttaa tccctaacgc ttccagtcgt gcctgtaaac
    30601 gccgaccaac gttaccaacg cctacgatcc ccacggtacg ctcgtgcagt gaaaatccat
    30661 cgcgttcggc aagcatcagc agggaggaga aaacatattc caccaccgca atcgcattac
    30721 agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa ccatgcttca tcgacatggt
    30781 cggtccccgc agtggcagtg ccaacaaatt taatgggttt tcctgccagc aaagattcat
    30841 tcactttcgt gaccgaacgc accatcagcg catccgcgtc tgccagttga gcgacgggga
    30901 ttggacgccc gggaaccgcg atcacctcac ccaaacggct aaataagtcg cgggcataag
    30961 gcatattttc atcaacaagg attttcacgt ttgtgttacc tgtatgagac gagagttaac
    31021 cggacaagtg tgccataatc tcgcggccag gcatacttgc gaagatttca ggtataagga
    31081 tacgtaatga tacaacctat ttccggccct cctcctgggc aaccaccagg tcagggagat
    31141 aacctgccgt ctggcgcggg caatcagcct ttatccagtc agcaacgtac ttcgctggaa
    31201 agcttaatga cgaaagtgac ctcactgacg caacagcaaa gagcagaact gtgggcgggt
    31261 atcaggcacg atattggtct gtcgggagat tcaccgctgc tttcgcgtca ctgccctgcc
    31321 gctgagcata atctggcgca acgtctgctg gccgcgcaaa aaagccattc tgcccgccag
    31381 cttttagcgc aattagggga gtatttacgt ctggggaata atcgtcaggc ggtcacggat
    31441 tatatccgtc ataactttgg tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa
    31501 accattctca ccctgttgca ggaagggaag atggttattc cgcaaccgca gcagcgcgag
    31561 gcgaccgatc gtcctctatt accggcggag cacaatgcgc tcaaacagct ggtgaccaaa
    31621 cttgcggcgg caacggggga gcccagcaaa cagatctggc aatcgatgct ggaactttcc
    31681 ggggtgaaag atggcgagtt aattccagcg aaactgttta accatctggt gacctggctg
    31741 caggcgcgtc agacgctaag ccagcaaaat acgccgacgc tggaatcact acaaatgacg
    31801 ctaaaacaac ctttagatgc cagtgaactg gcggcgttat cggcatatat ccagcaaaaa
    31861 tatggccttt ctgcgcaatc atcgctttct tctgcccagg ccgaggatat tcttaatcag
    31921 ctttatcaac ggcgggttaa agggattgat ccgcgtgata tgcaaccgct gcttaatcct
    31981 tttccaccga tgatggacac gttgcaaaat atggcaacgc gtcccgcgct gtggatactg
    32041 ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt aaccccgacg aaacgacagt
    32101 atcgtgacaa taattcccag caccgcagag atcgccccgg caagaaatac cgaagagtaa
    32161 ccaaacgtgg tcgccagcat tcccgcaagc ggcccggaga cgccgagggc gatatcctga
    32221 aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc
    32281 tccacgccca gcgcaggaaa gataagcgaa catccggctc cggttaacgc cgcgcccgct
    32341 aatgcgaccc acgcccctgg ggcttgccag agcagcaaca agcccaccgt ttctacaagc
    32401 agagagacaa tcgccacttt cacgccgcca aaacggtccg gcatccagcc aaacatgacg
    32461 cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa agcccgccat cgcccatcct
    32521 ttgctggcaa agtagagcga aacgaaagtc ccgataaccg caaaaccaac gccttgtagt
    32581 gccagaccta accctggttt ccagataagt ccgacaacgc tccacagaga tggacgttct
    32641 cccgccaggg ctggtacttt gcgcactgtg ccgttacagg cccacgccag taagggtaat
    32701 gccattgtgg tgagcgccag tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc
    32761 ggagcaccaa cagcgagggc accgtaaatc gccattccat tccatgacat cactttgcca
    32821 gagtgttttg gccctacgat gcctaacccc caggtcagag cgcctgtcag taactggctt
    32881 tcaccaaacc caagaatcaa acgcccgatg accaacaggg caaatttgaa cggtgcggag
    32941 acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca gaccacaagc taacatcccc
    33001 tgaagcgccg aacgttttgc gccatattga tcggccagtc gcccggcata gccacgcgtc
    33061 agcaccgtag ccagaaactg aatgccgacg gcaatgccga ccatggtatt gccatagccc
    33121 agatcatgat gaacaaacaa cgggataacc ggcaacggca accctacggt catgtaggtg
    33181 agaaaaaccg caaaagcgat gcggaagagc gaaaaattgg cagaagatcg tgtttcggtt
    33241 tggcttacag cagtcatgca ttactccaga atgcagcgca aggcgaggag tatccccgtc
    33301 tcatctctct ggtttcaggg ttacagtgcg ttggcaggat ttaacgcgta cgtcttttca
    33361 gaaggaaatc gacaaagcgg ggagtttgcc tggaactggc ggcgattgtc aatgatgtga
    33421 aaaagggaac catcaggttc ccttttgcgt tagtgccgga ggccgcattg gcgcgtaacg
    33481 tcggatgcga cgctggcgcg tcttatccga cctactccgg cctactgcga attaatcttt
    33541 cagcttgcgc attaccagcg tggcgttggt gccgccgaag ccgaagctgt tagacataac
    33601 agtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc agacccgcag cctgctcgtc
    33661 cagctcttca atgttgatgc tcggggcgat aaagccgtgt tccagcatca gcagagagta
    33721 gatagcttcc tgtacgccag cagcgcccag agagtgaccg gtcatggctt tggttgcaga
    33781 aatcgccggg ctcttatcgc cgaacacttc acggatagct gccagctctt tcacgtcgcc
    33841 aaccggagtc gaagtgccgt gggagttcag gtaatcgatt ggggtatcaa cgccatgcat
    33901 cgccatctgc atacaacgta ctgcgccttc gccagaggga gcaaccatgt ctgcgccatc
    33961 agacgttgcg ccgtagccaa cgatttcagc atagatgtga gcaccacgcg ccagcgcgtg
    34021 ttccagctct tcaaccacca ccataccgcc gccgccagcg ataacgaaac cgtcacggtg
    34081 agcatcgtaa gtacgggagg ctttttccgg ggtgtcgttg tatttagtag acagcgcacc
    34141 cattgcgtcg aattcgcaag ccatttccca gcacagctct tcgccgccgc cagcaaacac
    34201 gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta ccgatacagt gtgcggaagt
    34261 cgcacacgcg gagctgatgg agtagttaac gccatgaatt ttaaacggcg tggcgaggca
    34321 ggcagaaacg ccggatgcca tcgctttggt gaccacatac gggccaaccg ctttcaggcc
    34381 gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa cgcggggaac cgccgccaga
    34441 acctgcaatc aggccaacac gcggattatt ctggtaagct tccggagaga ggcccgcatc
    34501 tgcgattgcc tgctccatag aaaggaatgc ataaatggat gcgtcgctca taaagcgcac
    34561 aactttgcgg tcaatgaggc cagtggtatc cagttttacg ttgccccaga cgtggctacg
    34621 catgccggaa tccttcagct cctgagagaa agtgatccct gaacgtcctt cacgcagaga
    34681 tgccaggact tcctgctggt tattaccgat gctggaaaca atgcccaggc cagtaatcac
    34741 tgcacgtttc attcaatacc tctgtaagtc gcacatagag taagtttcga atgcacaata
    34801 gcgtacactt gtacgccgaa caagtccgat cagccaatta atagagaaat ttgcgcagcc
    34861 ttacacacat cgctaagatc gagctaccgc ctgtaagacg agtaacttac gtgaaacact
    34921 actccataca acctgccaac ctcgaattta atgctgaggg tacacctgtt tcccgagatt
    34981 ttgacgatgt ctatttttcc aacgataacg ggctggaaga gacgcgttat gtttttctgg
    35041 gaggtaacca attagaggca cgctttcctg aacatccaca tcctctgttt gtggtagcag
    35101 agagcggctt cggcaccgga ttaaacttcc tgacgctatg gcaggcattt gatcagtttc
    35161 gcgaagcgca tccgcaagcg caattacaac gcttacattt cattagtttt gagaaatttc
    35221 ccctcacccg tgcggattta gccttagcgc atcaacactg gccggaactg gctccgtggg
    35281 cagaacaact tcaggcgcag tggccaatgc ccttgcccgg ttgccatcgt ttattacttg
    35341 atgaaggccg cgtgacgctg gatttatggt ttggcgatat taatgaactg atcagccaac
    35401 tggacgattc tctaaatcaa aaagtagatg cctggtttct ggacggcttt gcgccagcga
    35461 aaaacccgga tatgtggacg caaaatctgt ttaacgccat ggcaaggttg gcgcgtccag
    35521 gcggcacgct ggcgacattt acgtctgccg gttttgtccg ccgcggtttg caggaagccg
    35581 gattcacgat gcaaaaacgt aagggctttg gacgcaaacg ggaaatgctt tgtggggtga
    35641 tggaacagac attaccactc ccctgctcca cgccgtggtt taatcgtacg ggcagcagca
    35701 aacgggaagt agcgattatc ggcggtggta ttgccagcgc attgttgtcg ctggcgctat
    35761 tgcgtcgcgg ctggcaagta acgctttatt gcgcggatga agcccccgcg ctgggtgctt
    35821 ccggcaatcg ccagggggcg ttgtatccgt tattaagcaa acacgatgag gcgctaaacc
    35881 gctttttctc taatgggttt acttttgctc gtcgacttta tgattcatta cccgttaaat
    35941 ttgatcatga ctggtgcggc gtcacgcagt taggctggga tgagaaaagc cagcataaaa
    36001 tcgcacagat gttgtcaatg gatttacccg aagaactggc tgtagccgtt gaggcaaatg
    36061 cggttgaaca aattacgggc gttacgacaa attgcagcgg tattacttat ccgcaaggtg
    36121 gctggctgtg tccggcagaa ctgacccgta atgtgctgga actggcgcaa cagcagggtt
    36181 tgcagattta ttatcaatat cagttacagg atttatcccg taaggatgac tgttggttgt
    36241 tgacgtttgc aggagatcag caagcgacac acagtgtagt ggtactggcg aacgggcatc
    36301 aaatcagccg tttcagccaa acatcgtctc tgccagtgta ttcggttgcc ggtcaggtca
    36361 gccatattcc gacgacaccc gaactggcaa aactgaaaca ggtgctgtgc tatgacggtt
    36421 atctcacgcc gcaaaatccg gcgaatcagc accattgtat tggtgccagt tatcatcgcg
    36481 gcagcgaaga gacggcgtac agtgaagaca atcagcagca gaatcgccag cgattgattg
    36541 attgtttccc acacgcacaa tgggcgaaga cggttgatgt cagtaaaaaa gaggcgcgct
    36601 gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt aggtaatgtt cccgattatg
    36661 aggcaacact cgtggaatat gcgtcgctgg cggagcagaa agataaggcg gtaagcgcgc
    36721 cggtttttga cgatctcttt atgtttgctg ctttaggttc gcgcggtttg tgttctgccc
    36781 cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga accgattccg atggatgcca
    36841 gtacgctggc ggcgttaaat ccgaatcggt tatgggtgcg gaaattgttg aaaggtaaag
    36901 cggttaaggc cgggtaatct gctctcgcag tgtttgccgg atgcggcgtg aacgccttat
    36961 ccgacctacg tgtgacgtgt aggcctgata agacgcgata agcgtcgcat caggcatggt
    37021 gctccagacg ccgcaactta ctgctgtgac gcctgctgaa acaggttttc ccacatatcg
    37081 gtcaccagcg cctggtcacg cggcgacaat tcaccagcac caatggcttt ttccagactc
    37141 tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat caccactttc cagttctgcg
    37201 atggctaatg taaggtggcc acgcaaatac ccactggcaa acaactcatc atcactggca
    37261 tggtcaacca taccgtcgat taattccaga atgcgtgatt caaactccgc gatcatcttc
    37321 tttcctcatt gtaaaacgtg gcgctggctt cagttgagcg cttccggcca cgggaactgt
    37381 tccgccgtaa gttccggcgt gtgataataa ttttgtagtg ctttaatgaa acgtgccggg
    37441 cgttccggaa tgccatttgt tagataatcc atcacctgcg catgtactcg ccgttgaaac
    37501 accacgcggt ccggttcgaa gtcgccttcc agattgtcgc aactgacgtt aaacgggtat
    37561 cccgccgcca cgcagaacaa ccagtcgagc gcctgcggct tcacttcaac atcttcaaac
    37621 tggctttgcg tttgggcatc gcgaccgtcc gggcaatacc agtagccgaa atcaacctgc
    37681 tcgcggcgcg ctttcccggc aatacaccag tgcgaaatct catgaattgc gctggcataa
    37741 aagccgtggg caaagacgat tcggttatac ggtacttccg catcagcggg aagatagatc
    37801 ggttcgtcgt cgcctttaat cagacgggta ttaaaatcat cggcaaagca gctattaaaa
    37861 atttcaatca actgctcata gtggtgtgta ctgttcatta gttcatcccc aaccagtgga
    37921 ggatctcctg tccgtggcta tcataaagta atttggcact catcaccgcc gagacgataa
    37981 cgatcatcgg gcggatcagt ttttgtcctt tgctcaacac cagtcttgaa cccatgcgcg
    38041 cgccaaggaa ctgccccacc agcatcacaa agcccgtcgc ccaaatcact ttgccgccga
    38101 gaataaacag cagtaaaccg ccgatgtttg acgttgcgtt gagtaatttg gcgtgagccg
    38161 tggctttggc gaggttgaat ccgcacagcg taacaaaggc cagcgcgtaa aacgatccgg
    38221 ctgccggacc aaagaatccg tcataaaaac cgacgcagcc accggcaatt aacgcgaagg
    38281 gcaaaccgta catccggcgc tggcgatctt cttcacccag ctttggcatc agcaagaaat
    38341 agaggccgat acaaatcacc agaatgggca aaatctgccg taagacatca gcctgaacgt
    38401 actgtaccaa cagtgcgccg ctcattgagc cgacaaaggt catggcaata ttaagtttct
    38461 gatcgcttaa actaaccact ttgcggcgaa taaagtagat agtggcggaa atagagccgc
    38521 cgcaggcttg cagtttattg gttgccagag cattagcggg agacatcccc gctgccatca
    38581 atgccggaat ggtgagtaac ccaccgccac cggcaatcga gtcgataaat cccgccagca
    38641 tggcgacaaa aaagagaact cccagcaaca gcggggaaac cataaacagg ctattaaacg
    38701 tttccattag atcacgtgct catccagtag cgcctggcag gaaggcggca acggaggcgg
    38761 tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt tcaaaccagc tttgcagttc
    38821 tgccccgcaa ccatcgcctg gtggcggtaa aggttgatct tcacactcca gactatcggc
    38881 aggacaacgt aatcgtacat gcatatgcgc acgatgctgg aaccagggtc gcactttgcg
    38941 caaccagtcg cgatcggtgc ccgcatcaag gcaaagttgt tgtttaatcg ccggattaac
    39001 aaaaatgcgc gtgacgtctt tgtcctgggc ggcgagtttg atcaagctga aaatttctgg
    39061 cttccacagc gtggagacaa cgtgtttacc gtcgcgggaa actaagtcca gtgcttgcgg
    39121 gcgcaagagc tgcgcggagg tccggcgtgt tttcggcagt tgcagaaaga tatcgacatc
    39181 cagtccggtc tggtggctgg cgtgaccgcc gttgaaacgc ccaccagcag gcattcccat
    39241 atcgccaatc aacaccgtac ccatgcccag attgctcacc tgactactca ggcgctggat
    39301 aaacatcacc aggtccgggt gaccgaaata gcgacgctga tcggtacgca tgacctgata
    39361 atgttcggac tgtatcggca acgtgtcagc gccgacgata caaccattgg aaaaactgcc
    39421 tatcgattgt gcgctacccg gcacaggttg ggttattttt tgccacggcg tcgctgccag
    39481 gctgacgcta ctggcaagca gggccagcag cgcaatcgcg gttttgttca ttttttacca
    39541 gcgtggaata tcagtcttca catcggcatt ttgcgcccgc tgccgtaaca ggtgatccat
    39601 taaaacgatc gccagcatcg cttctgctat cggcaccgcg cggatcccga cacagggatc
    39661 gtgacggcct ttggtgatca tctcaacttc ttcgccaaag cggttaatgg tgcgccccgg
    39721 cacggtaata ctggaggtcg gtttcagtgc catatgagca atgatttgct gcccgctgct
    39781 aattccgccg agaatgccgc ccgcatggtt actctggaaa ccgtctttgg tgatttcgtc
    39841 gcggttctgg ctgccacgca gcgccaccac gtcaaaacca tcaccaattt ccacgccttt
    39901 caccgcgtta atgctcatta gcgcatgggc gatgtcggca tccaggcgat caaagaccgg
    39961 ctcaccaagc ccggcaggaa cgccactggc aacaacggtg actttcgcgc caatagagtc
    40021 gccctctttt ttcagcgcgc gcatcagttc atctaacgcg tcgattttgt ccgggtccgg
    40081 gcagaagaat ggattttgct cgacctgcga ccagtctttg atttccagcg gaatgtcgcc
    40141 catctgggtc aggcagccgc gaatttcgat gccaaatttc tcagcgagat attttttggc
    40201 aatcgcccct gctgcaacgc gcatggcggt ttcacgggcg gaagaacgtc caccgccgcg
    40261 ataatcgcgc agaccgtatt tttgttcgta ggtgtaatcg gcatggccag gacggaaaac
    40321 gtccttaatc gcactataat cctgagaacg ctgatcggta ttttcgatca acaaaccaat
    40381 gctggtgccg gtagtaacac cttcaaaaac accggagaga attttgacct gatctggctc
    40441 acgacgctgg gtggtatagc gcgatgtccc agggcgacga cggtcgaggt catgttgcag
    40501 gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg acgatgcagc cgagcgccag
    40561 cccgtgcgat tcgccgaagg tggttacgcg aaagagttgt ccaattgtgt ttccagccat
    40621 cacggctccg ttattgttgt gtttgcgtgt ttacttaatc tttataaatc gcgaaatgtt
    40681 cacgggccgc cagcagctgc gctttggtga gcataaacac accatcgccg ccgttatcaa
    40741 actccagcca ggtgaacgga acatcgggat attgttccat aagatgtacc atgctgttgc
    40801 cgacttcaca aatcagtacg ccatcatcag caagataatc tgccgcgttt ccgagaatgc
    40861 gacgcgtcag tttcaggccg tcggtgccag atgccaggcc cagttccggc tcgtggcggt
    40921 attcatttgg caggtcggac atatcttccg catcgacata cggcgggtta gtgacaatca
    40981 ggtcgtactg cactttcggc aaatcacgga acagatcgga acgaatcggt atgacgttgt
    41041 ggatcagacc gtgttcttcg atgttctgtt cggcaaccgc cagcgcgtcg ggagagatat
    41101 ctaccgcatc gacttccgct tccgggaagg cataagcaca ggcaatggcg atgcagccgc
    41161 taccggtaca catatctaaa atatgctgcg gttgcttgct gataagtccg gcaaatttat
    41221 tgttgatcaa ttcaccaatc ggcgagcgcg gcaccagcac gcgttcatcg acgtaaaatt
    41281 catggccgca gaaccacgct ttgttggtca ggtaagctac cggaatgcgt tcgttgacgc
    41341 ggcggatcac gcgctcaaca atacgatgtt tttcgctgga ggtcagacgc gcggtgcgca
    41401 tatcttccgg aatatccagc ggcaggtaga gcgacggcaa taccagctgt acggcttcat
    41461 cccacgggtt atcggtgccg tgaccgtacc agatatttgc cgcgctgaag cggctcaccg
    41521 accagcgcaa catgtcctga atggtttgca gctcatttac tgcttcatca acgaaaattt
    41581 tatccacgta ttcctccagg gcatgatcgc aataatttcg gcggctagtt tgccatgaag
    41641 atgacgataa atcagcattc acgcgcggtg agtgaggaaa aatacgttta aaacgatcga
    41701 ttgcgctacg agtcgggtaa actgtaggaa aattagaaat agagacagat aaatgaaaaa
    41761 gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc cagttgatgg cggggactcg
    41821 caagattaag caggacacca ttgtccatcg gccacagcgt aaaaaagtca gcgaagtgcc
    41881 cgtcaaacgg ttgattcagg agcaggttga cgccagtcac tatttctctg acgaatttca
    41941 accgttatta aatgctgatg gcccggtgaa gtatgtacgc cctggtgtag accattttga
    42001 ggcgaaaaag ctgcgccgcg gcgattattc gccggaattg tttttggatt tacacggtct
    42061 gacgcagctg caggccaagc aggaactggg cgcgttgatc gccgcctgtc gccgcgaaca
    42121 tgtgttttgc gcctgcgtga tgcatggtca cgggaaacat atattgaagc aacaaacacc
    42181 gctgtggctg gcacaacatc cgcatgtcat ggcgtttcac caggcaccaa aagagtatgg
    42241 tggtgatgcc gcattgctgg tgttgattga agtggaagag tggctaccgc cggagttgcc
    42301 atgaatatta gggggcggat gcgttttgcc atttcgccct gaatgctggt atcgaatgcc
    42361 ggatgcggcg taaacgcctg atccggctta tcaaaatgtg caaattcaat acgttgtgat
    42421 tcagttgtag gcatgataag acgcgtcagc gtcgcatcag gcatcaacgc acaactacag
    42481 ttcattcagt acatactgga aacggcatgt agaatcaatt ggctcacggc tactggtatt
    42541 cacctgtacc acaatgtcgt gtagctttct atccgtcaat ggttgacgtg agggggtgac
    42601 tgtcaccttt gcattatcac aaccatacga ggtaaaggtt aataagtcag aaacagcacg
    42661 acaatatgcc cctctggaat caaaaatact gccatcgcag agtttgccgc tttccatcag
    42721 gtaggtcgaa agcgtagtcg tgattgtcga accatcggat tgcaatcgca gcgctttatt
    42781 atggctaaca taattatcac tccaggatat atcaaacgaa gcgacgggaa ttgtcgggca
    42841 aatagaatca tgaactgttg ggcttgtata cgtaccaaat ccaaatgcct cagatactga
    42901 ataaaatgag gcaatccacg gacaaccata attatcagat tctacagtga cgttaaaagt
    42961 catatatgca cagacaaaga cacgagtatt gataccaggg caatttgagg tgtccgtacg
    43021 tctataatca gtcaaaacca gtgtcggtgc cgggccagat ccgctccagg atattaactt
    43081 gagggtgtcg ccttttcttg cattggagtt gtattgtccg tagcttactt ctcccggcgt
    43141 cactaccgtg acatagaaat ataaactgcc ggaagcagta acgtttttca tcgcataata
    43201 aattttagta ctggaaaagc ttgcactcat gagtttatca ccggcatagc actttggtgt
    43261 ttctaaact
//
LOCUS       DAZKFZ010000014        42551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000014 DAZKFZ010000000
VERSION     DAZKFZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            56..1309
                     /gene="ynfM"
                     /locus_tag="ACHOQW_000744"
     CDS             56..1309
                     /gene="ynfM"
                     /locus_tag="ACHOQW_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9571871.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1358..1477)
                     /locus_tag="ACHOQW_000745"
     CDS             complement(1358..1477)
                     /locus_tag="ACHOQW_000745"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP9571872.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1733..2041
                     /gene="asr"
                     /locus_tag="ACHOQW_000746"
     CDS             1733..2041
                     /gene="asr"
                     /locus_tag="ACHOQW_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP9571873.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            2134..2217
                     /locus_tag="ACHOQW_000747"
     CDS             2134..2217
                     /locus_tag="ACHOQW_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9571874.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHOQW_000748"
     CDS             2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHOQW_000748"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP9571875.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACHOQW_000749"
     CDS             complement(3177..3506)
                     /gene="mdtI"
                     /locus_tag="ACHOQW_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP9571876.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHOQW_000750"
     CDS             complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHOQW_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP9571877.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4270..5304
                     /gene="tqsA"
                     /locus_tag="ACHOQW_000751"
     CDS             4270..5304
                     /gene="tqsA"
                     /locus_tag="ACHOQW_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP9571878.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="ACHOQW_000752"
     CDS             complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="ACHOQW_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP9571879.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="ACHOQW_000753"
     CDS             complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="ACHOQW_000753"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP9571880.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8784..9728
                     /gene="ydgH"
                     /locus_tag="ACHOQW_000754"
     CDS             8784..9728
                     /gene="ydgH"
                     /locus_tag="ACHOQW_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP9571881.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9914..11296
                     /gene="ydgI"
                     /locus_tag="ACHOQW_000755"
     CDS             9914..11296
                     /gene="ydgI"
                     /locus_tag="ACHOQW_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9571882.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11333..12055
                     /gene="folM"
                     /locus_tag="ACHOQW_000756"
     CDS             11333..12055
                     /gene="folM"
                     /locus_tag="ACHOQW_000756"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP9571883.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12052..12387)
                     /gene="ydgC"
                     /locus_tag="ACHOQW_000757"
     CDS             complement(12052..12387)
                     /gene="ydgC"
                     /locus_tag="ACHOQW_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP9571884.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12516..13235
                     /gene="rstA"
                     /locus_tag="ACHOQW_000758"
     CDS             12516..13235
                     /gene="rstA"
                     /locus_tag="ACHOQW_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP9571885.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13239..14540
                     /gene="rstB"
                     /locus_tag="ACHOQW_000759"
     CDS             13239..14540
                     /gene="rstB"
                     /locus_tag="ACHOQW_000759"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP9571886.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14616..15545
                     /gene="tus"
                     /locus_tag="ACHOQW_000760"
     CDS             14616..15545
                     /gene="tus"
                     /locus_tag="ACHOQW_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP9571887.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15542..16945)
                     /gene="fumC"
                     /locus_tag="ACHOQW_000761"
     CDS             complement(15542..16945)
                     /gene="fumC"
                     /locus_tag="ACHOQW_000761"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP9571888.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17088..18734)
                     /gene="fumB"
                     /locus_tag="ACHOQW_000762"
     CDS             complement(17088..18734)
                     /gene="fumB"
                     /locus_tag="ACHOQW_000762"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9571889.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18933..20108
                     /gene="manA"
                     /locus_tag="ACHOQW_000763"
     CDS             18933..20108
                     /gene="manA"
                     /locus_tag="ACHOQW_000763"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP9571890.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20209..21717
                     /gene="ydgA"
                     /locus_tag="ACHOQW_000764"
     CDS             20209..21717
                     /gene="ydgA"
                     /locus_tag="ACHOQW_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9571891.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(21762..23026)
                     /gene="uidC"
                     /locus_tag="ACHOQW_000765"
                     /pseudo
     CDS             complement(21762..23026)
                     /gene="uidC"
                     /locus_tag="ACHOQW_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(23065..24438)
                     /gene="uidB"
                     /locus_tag="ACHOQW_000766"
     CDS             complement(23065..24438)
                     /gene="uidB"
                     /locus_tag="ACHOQW_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP9571892.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(24435..26248)
                     /gene="uidA"
                     /locus_tag="ACHOQW_000767"
                     /pseudo
     CDS             complement(24435..26248)
                     /gene="uidA"
                     /locus_tag="ACHOQW_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(26639..27229)
                     /gene="uidR"
                     /locus_tag="ACHOQW_000768"
     CDS             complement(26639..27229)
                     /gene="uidR"
                     /locus_tag="ACHOQW_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP9571893.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(27458..28225)
                     /gene="hdhA"
                     /locus_tag="ACHOQW_000769"
     CDS             complement(27458..28225)
                     /gene="hdhA"
                     /locus_tag="ACHOQW_000769"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP9571894.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(28337..29365)
                     /gene="malI"
                     /locus_tag="ACHOQW_000770"
     CDS             complement(28337..29365)
                     /gene="malI"
                     /locus_tag="ACHOQW_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP9571895.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            29540..31132
                     /gene="malX"
                     /locus_tag="ACHOQW_000771"
     CDS             29540..31132
                     /gene="malX"
                     /locus_tag="ACHOQW_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP9571896.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            31142..32314
                     /gene="malY"
                     /locus_tag="ACHOQW_000772"
     CDS             31142..32314
                     /gene="malY"
                     /locus_tag="ACHOQW_000772"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP9571897.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            32418..33419
                     /gene="add"
                     /locus_tag="ACHOQW_000773"
     CDS             32418..33419
                     /gene="add"
                     /locus_tag="ACHOQW_000773"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9571898.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(33455..34495)
                     /gene="ydgJ"
                     /locus_tag="ACHOQW_000774"
     CDS             complement(33455..34495)
                     /gene="ydgJ"
                     /locus_tag="ACHOQW_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9571899.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            34738..34863
                     /gene="blr"
                     /locus_tag="ACHOQW_000775"
     CDS             34738..34863
                     /gene="blr"
                     /locus_tag="ACHOQW_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP9571900.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            35136..35351
                     /gene="ydgT"
                     /locus_tag="ACHOQW_000776"
     CDS             35136..35351
                     /gene="ydgT"
                     /locus_tag="ACHOQW_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP9571901.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            35437..35877
                     /gene="ydgK"
                     /locus_tag="ACHOQW_000777"
     CDS             35437..35877
                     /gene="ydgK"
                     /locus_tag="ACHOQW_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP9571902.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            35954..36535
                     /gene="rsxA"
                     /locus_tag="ACHOQW_000778"
     CDS             35954..36535
                     /gene="rsxA"
                     /locus_tag="ACHOQW_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP9571903.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            36535..37113
                     /gene="rsxB"
                     /locus_tag="ACHOQW_000779"
     CDS             36535..37113
                     /gene="rsxB"
                     /locus_tag="ACHOQW_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP9571904.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            37106..39424
                     /gene="rsxC"
                     /locus_tag="ACHOQW_000780"
     CDS             37106..39424
                     /gene="rsxC"
                     /locus_tag="ACHOQW_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9571905.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDP
                     RKAAVEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARKLEQQQANAEP
                     EEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
     gene            39425..40483
                     /gene="rsxD"
                     /locus_tag="ACHOQW_000781"
     CDS             39425..40483
                     /gene="rsxD"
                     /locus_tag="ACHOQW_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP9571906.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            40487..41107
                     /gene="rsxG"
                     /locus_tag="ACHOQW_000782"
     CDS             40487..41107
                     /gene="rsxG"
                     /locus_tag="ACHOQW_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP9571907.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            41111..41806
                     /gene="rsxE"
                     /locus_tag="ACHOQW_000783"
     CDS             41111..41806
                     /gene="rsxE"
                     /locus_tag="ACHOQW_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP9571908.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            41806..42441
                     /gene="nth"
                     /locus_tag="ACHOQW_000784"
     CDS             41806..42441
                     /gene="nth"
                     /locus_tag="ACHOQW_000784"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9571909.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT        10347 a        11010 c        10485 g        10709 t
ORIGIN      
        1 ttttcctaac ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacat ttgcacttct ctattgtgtg caacctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttccacggc
      301 gatgttggct attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc
      361 agtgatggta acggcgctac tattggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc
      481 agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt
      601 cttcacggac tttttcaact ggcgaattgc tctggcggct atcggttgtt tcgcgctagc
      661 ctcggcgttg atgttctgga aaatcctccc ggaatcacgc cattttcgcc cgacttcgct
      721 gcgacctaag tcgttgttta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
      841 ctatcggttg atgctctccc cctggcatgt cagtcaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctatgg
      961 gcgtggtcca gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt
     1021 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc
     1081 agcccactca gttgccagca gctggatcgg cccccgcgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagtcc
     1321 ggactgcggt aaatacccgt ccggacttat tgcctgctca aaccaacgtt aatagccatc
     1381 ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa
     1441 gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc
     1501 taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa
     1561 attttcctta agtcacaata cgttattatc aacgctgtaa tttattcagc gtttgtacat
     1621 atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg atatagttct
     1681 ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa
     1741 agtattagct ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga
     1801 gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgccgg cgaaaactac
     1861 acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca
     1921 tcataaaaat acgaaagctg aacagaaagc cccagaacaa aaagcgcagg cagcgaagaa
     1981 acacgccaag aaacacagcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcgta
     2041 agttttcaac agtaatgctg gcgcgccccc tcgcgcctga aaattacggt gctaagcggg
     2101 taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc
     2161 attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct ttcctgatcg
     2221 tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt
     2281 gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg
     2341 ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa
     2401 tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt gccagtgaat
     2461 gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta
     2521 tgtacggcga cgctgattgc acccaatctg gcattaacgg caggacactg tttattgacg
     2581 ccaccaaagg gtaaagccga taaagcagtg gcgctgcgtt ttgtgtcaaa taaaggtctt
     2641 tggcgctatg acatccacga catagaaggc cgcgttgatc cgacactggg aaagcggtta
     2701 aaagcagatg gggatggttg gattgtacct cccgcagccg cgccgtggga cttcggattg
     2761 attgtgctac gtaatccccc ttctggcatt acgccgttgc cgttatttga gggagataaa
     2821 gccgcgctta ccgccgcatt aaaagcggca ggtcgtaaag tgactcaggc aggataccct
     2881 gaagatcatc tcgatacgtt gtacagtcat caaaactgtg aagtgactgg ctgggcgcaa
     2941 acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctctgatg
     3001 ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa
     3061 gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat
     3121 caactgtcgc aaaaataatg ttcaaacgct gcccgacagc gcgggcagcg tcttcatcag
     3181 gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaatcca gcctttacga
     3241 tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg
     3301 ccccacaatg cataagcgac agacaagtcg atccctttaa cggcctgaga aagcgcacta
     3361 aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca
     3421 gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg
     3481 gcgtgaaccc attcaaactg cgccatggtt cacctccagt tcaggtttac gtgctttacg
     3541 ggtacctgat ttgatcaaca ctatcccggc gaccagggtg gttaacccgg caattttcat
     3601 cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata aaataccgat
     3661 accttcccac agcgcataag ccacacctaa ggctattttt ttaacagcga aagagagaaa
     3721 tatatacgac agagaaatca tcaccagcat taaaataaag ccgccatttc cctcactgac
     3781 gctcgcccat ttcatagaca gcgtaccggt aatttctgta gcaatagcca gacctaataa
     3841 aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt ttcgcttaat
     3901 tcagcgaaac caaagtaaag tggtcaagcc ccgcgtatag ggcaagctta ggcagaagaa
     3961 aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac
     4021 gaagatgggt aaatgtagaa aataacgtcc tgaacaaatt gtccataata ttacaattat
     4081 ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg
     4141 atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt
     4201 gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaacg
     4261 gggacaagca tggcaaagcc gctcatcacg ctcaatggcc taaaaatcgt cattatgttg
     4321 ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt
     4381 attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg
     4441 cgtgtgccgc gtgtactggc agtgtcgatt ttgatgacca tcatcgtgat ggctatggtg
     4501 ttgctgttag cttatctggg ttccacgctc aacgagttga cgcggacgtt accgcaatat
     4561 cgcaactcta ttatgacgcc gctgcaagct cttgagccgt tgttgcaacg cgtagggatt
     4621 gacgtctcag ttgaccaact ggcgcattac attgatccga acgcggcgat gacgttgctc
     4681 accaacttat tgacgcagtt atctaatgcc atgtcatcaa tatttttatt gctgctgacg
     4741 gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttaagca aatgatggcg
     4801 cgtccggttg aagggatggc ggcgattcag cgtgcgattg acagtgtgtc tcattatctg
     4861 gtgctgaaaa cagccatcag catcatcacc ggcctggtcg catgggcgat gctcgccgcc
     4921 ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatccct
     4981 aatattggtt cagttctcgc ggcaatcccc cctattgctc aggtactggt gtttaatggc
     5041 ttctacgaag cgttgttggt gctggcggga tatctgctga ttaatctggt cttcggcaat
     5101 attctggagc cacgcatcat ggggcgtggg ctgggacttt ccacattggt ggtatttttg
     5161 tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgtta
     5221 acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg
     5281 ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt
     5341 caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa
     5401 gaacagcggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac
     5461 gttctgcgct ttccacactt ccagcacagg cataccagca atcggactct tcggatcatc
     5521 ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc
     5581 aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc
     5641 cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac
     5701 gttgatacca cgagcgcgca gtttctcggt aatttcagcg acaggatatt gcgcctgcgc
     5761 gactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca acagttccgc
     5821 tgtctcttct gcggtgattt cgcggtgctc acccacttcc tgatcatcgc cagtagaaga
     5881 gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca
     5941 cataatgtaa gaaaggatag cccccgagga accgaccagc gcaccggtca caatcagcag
     6001 gtcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat
     6061 cgacaccacc accggcatat ctgcaccacc gatggaggcg actaaatgcc agccaaatac
     6121 cagcgcaatg gcggtcatta ttagcaatgc cagcacttgc aggccgacgc tatccgtgcg
     6181 aacaaataca atcagcagca ggaaggaaac gaccagagct gccaggttca ttttgtgacg
     6241 gtttggcagc atcaacggtt tagacgaaat tttgccacac agtttgccga acgccaccac
     6301 cgaacccgtg aacgttaccg ccccgatgaa gataccgaga aacacttccg tcaggtgaat
     6361 attgaccaga atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa agccaaccag
     6421 cactgccgcc agacccacga agctatgcag gatcgccacc agttctggca tttcggtcat
     6481 ttcaactttc ttcgccagac ggataccaat tgccccacca atgaccatcg ccagcaagat
     6541 ccagccaacg ttacccgtat ccggtccaaa aatggtggcg attagcgcaa tcgccatccc
     6601 ggcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagcc cggccagact
     6661 gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg
     6721 ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga
     6781 aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct
     6841 ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca
     6901 tcaacggtgt atgcagcgcg tgcgatacgt tccacaccac gtaataaccg acaacgcagg
     6961 ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg cgcaacgctt gccagccagc
     7021 cgaaaaggat gattgccagc gccatcaacg cgtatttacg ccacggtgaa caggcacatt
     7081 tttcctcagt tttcacttcc ggtgccgctt tttgtgccac ctgcggctga gctgatacct
     7141 gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacagtcacg ccgcgaatca
     7201 ccacatcatc aaaatcaaca gtgatgttac cgtttttctc tttgcacaac agtttcagca
     7261 gattaacgag gttagtgccg taaagctgtg aggattgcgt cggcagacgg cccggaagat
     7321 cggtatagcc aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt
     7381 attcacagtt gccgccgttt tgggctgcca ggtcgacaat cacactgccc gccttcatgg
     7441 agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg
     7501 tgacaatgat atcgacctct tttgcctggg cggcaaagag ttccatttcc gctttgatga
     7561 acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca
     7621 gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga
     7681 atgcacgcac aatcgcgccg agactgtttg ctgcgccaat ggcggccaga cctgcaacac
     7741 ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagta atttgcccgg
     7801 taaagaagca cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatattcg
     7861 ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gagtccatcg
     7921 ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc
     7981 agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg
     8041 cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc
     8101 ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg
     8161 cgacggtaaa ccccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg
     8221 gttcattggt taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt
     8281 ttattgatga tggtttgcct gtgtcaggag ccacacaagc cgctcatgta cgagctaaat
     8341 gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg
     8401 tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc
     8461 gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt cttcgacggt
     8521 cgggtaaaac gagacatcgc cccggcacga atcactactt aacattaaat taacttatac
     8581 aattcagttg cttcagtagt aatgatgctg atacggctgt tttttaagca tagacggtca
     8641 tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac
     8701 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
     8761 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
     8821 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
     8881 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
     8941 tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca
     9001 cttctgattt tgggaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
     9061 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
     9121 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
     9181 agccagaagt caatgatgcc atcaccaaag cggcaaaagc gaaaggtgcc tactctttct
     9241 acatcgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct
     9301 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg
     9361 aagcaggacg tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt gagatcccgg
     9421 gtgttgcgac taccgcatca ccaagttctg aagtcggtcg cttctttgaa acccagtcat
     9481 caaaaggcgg gcgttacact gtcacgcttc cggatggcac taaagtcgaa gaactgaaca
     9541 aagcgacagc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
     9601 acatgaccga agtctcctat caggttgcga aacgagccgc gaagaaaggt gctaaatatt
     9661 accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagt gcagatctgt
     9721 ataaataact attcagttac tcttataagg cggcttgatg ccgcctttcg catttttagt
     9781 cacaaatttc cccgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca
     9841 gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat cacgttttaa
     9901 tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag
     9961 ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc
    10021 ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc tggcctttgc
    10081 catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg
    10141 cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgcgcagt
    10201 catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac
    10261 gccggaattg cgcctatttg gcgatggtaa tacctggcaa tcgatagtcg gtgcatcggc
    10321 tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg ccagcattaa
    10381 cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc tggcgatgat
    10441 gatgttcaaa ctggatatct tcaagctcga cttcaccgga cttgcacttg gcgtacctgt
    10501 ttgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg
    10561 tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct
    10621 ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggagtggt
    10681 ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga tggtggaaat
    10741 gatgggaccg tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggcgc
    10801 gtacttaagc tggaccatca tggcggcaga agttccgttc ctggctgcca ctcataaagc
    10861 attcccacgc atttttgcgc gccagaacgc acaagctgcg ccatccgcct cactgtggtt
    10921 gacaaacatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt ccgattacaa
    10981 cacgttgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt
    11041 cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta
    11101 tggcttatgg ttattgtacg cctctggtcc aatgcacttg ctgttatccg ttgttctgta
    11161 tgcaccgggg ctgctggttt tcctgtatgc gcgcaaaacg catacacatg ataatgtact
    11221 gaaccgtcag gaaatggtgc tgattggcat gctgttaatt gcctcagttc cggcgacctg
    11281 gatgctggtg ggataacgtt catcccatcg ttggatagca aaggagataa cgatgggtaa
    11341 aacccagccc ttgccaatat taattactgg cggaggtcgt cgcatcggcc tcgccctcgc
    11401 atggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacac actatccagc
    11461 cattgatgga ctgattaatg caggtgcgca gtgtattcag gctgattttt cgaccaacga
    11521 cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc gtgctatttt
    11581 gcataacgcc agtgcgtgga tggcggaaaa accgggtgcg ccactggccg acgtactggc
    11641 ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgcgc tggaaagatt
    11701 actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga
    11761 gcgcggtagc gacaaacata ttgcgtatgc tgcaagcaaa gcggcactgg ataatatgac
    11821 ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct
    11881 gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga ataaatcatt
    11941 gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag
    12001 ttgctttgtc accggacgca gtttcccact tgatggcggt cgtcatctgc gttaatgcag
    12061 ctttatccag catataatca acacccatgc gcttatcccc caacacgcaa cagaaccaac
    12121 aaaagcggcg ggcaagcgca tcattccggt gaaataccac agcgacacca ggtaaacaaa
    12181 atagggaata atcgaccaca tactaaaaat gatggttgcg cgtaaggctt caatgccgcg
    12241 ttcgctggca acaatataat gcgcgataag cgcaaaggtc gggaaaagtg gaatcagccc
    12301 ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc
    12361 gagcgcggct ttgattacca gccccatctt tttaccttaa cacttccata acaagtcatc
    12421 agtagaatac ctgatgaaaa cttgtttaga aacgattgat agtaagtaaa aacagcgcgg
    12481 tgtattgtga cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag
    12541 atgatgcgga agtcggttca ctgattgccg cgtacctggc aaaacatgat atacaggtta
    12601 ccgtagagcc gcgcggcgac caggccgaag aaaccatttt gcgagaaaat ccggatttgg
    12661 tgttactcga catcatgcta ccaggcaagg acggcatgac catttgtcgt gatttacgcg
    12721 caaagtggtc cggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc
    12781 tggcactgga aatgggtgcc tgcgactata ttctcaaaac gacgccccct gctgttttgt
    12841 tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc cacgctgacc aaaggtcttc
    12901 aggaaacgtc actgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccta
    12961 tcaaccgcgt agtcaccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat
    13021 tattgtggga attagctacc catgccgggc aaatcatgga ccgcgatgca ttgctgaaaa
    13081 atttacgcgg cgtcagttat gacggcctgg atcgtagcgt ggacgtggct atttcgcggt
    13141 taagaaaaaa actgctcgat aacgccgctg aaccttatcg cattaaaact gtgcgtaaca
    13201 aaggctatct ttttgcgcct catgcatggg aataagcgat gaaaaaactg tttatccagt
    13261 tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggttggg ttggtgtaca
    13321 aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac agttcgctgt
    13381 atctgatgcg cagcgaattg cgtgagatcc ccccacatga ctggggtaag acgctgaaag
    13441 agatggattt aaatctctct ttcgatctgc gagtcgagcc actgagtaaa taccatcttg
    13501 atgatatttc catgcaccgg ctgcgtggcg gcgaaattgt cgccctggac gatcagtaca
    13561 cttttttgca gcgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc
    13621 tttattatct ccatcagatg cgattgctgg atatcgccct gatcgctttt attgctattt
    13681 ccctcgcctt tccggtgttt atctggatgc gtccgcactg gcaggatatg ttaaaactgg
    13741 aagcagcggc gcaacgattt ggcgatgggc atctcaatga acgtatccac tttgatgagg
    13801 gttcgagctt tgaacgactt ggtatcgcat ttaaccagat ggcggacaat atcaacgcct
    13861 tgattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag
    13921 tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgccgaa tcccaggcgt
    13981 tgaatcgtga tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac
    14041 tcgatcgccc acaaaacgag cttcatctta gcgaaccaga cctgccgttg tggctgtcaa
    14101 cgcatctggc agatattcag gcagtaacgc ccgataaaac ggtacggata aaaacgctca
    14161 tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc
    14221 tcaataatgc cctgcgctac tgccattcaa cggttgaaac cagcctgcta ctgtcgggga
    14281 atagagcgac attaattgtt gaggatgatg gcccagggat tgccccagaa aaccgcgaac
    14341 atatctttga accttttgtt cgcctcgatc ccagccggga tcgctcaacc ggcggctgcg
    14401 ggctggggct ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg
    14461 acaccagcga actgggtggt gcccgcttct cgtttagctg gccgttatgg cataacatcc
    14521 cgcaatttac ctctgcttga cactacgcgc acggtggtca agtcaccacc actgtgctat
    14581 aaattaagga tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc
    14641 gactcaacac tacttttcgc cagatggaac aagagctggc tgcatttgcc gctcatcttg
    14701 agcaacacaa gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg
    14761 agcataatcc gcttaatcgt attgaggtga aacaacatct cggcaacgac gcgcagtcgc
    14821 aggcgttgcg tcatttccgt catttattta ttcaacaaca gtccgaaaat cgcagcagca
    14881 aagcagctgt ccgtctgcct ggcgtgttgt gttaccaggt cgataacctt tcgcaagcag
    14941 cgttggtcag tcatattcag cacatcaata aactcaagac cacgttcgag catatcgtca
    15001 cggttgaatc agaactcccc accgcggcac gttttgaatg ggtgcatcgt catttgccgg
    15061 ggctgatcac ccttaatgct taccgcacgc tcaccgttct gcacgatccc gccactttac
    15121 gctttggttg ggctaataaa catatcatta agaatttgca tcgtgatgaa gtcctggcac
    15181 agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc gaggagtggc
    15241 aaagaaaact ggagcgagag tatcaggata tcgctgccct gccacagaat gcgaagttaa
    15301 aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa ggagatcaaa
    15361 aacaagtcca acacgcctgc cctacaccgc tgattgcact gattaatcgg gataatggtg
    15421 cgggcgtgcc ggacgttggt gagttgttaa attacgatgc cgacaatgtg cagcaccgtt
    15481 ataaacctca ggcgcagccg cttcgtttga tcattccacg gctgcacctg tatgttgcag
    15541 attaacgccc ggctttcata ctgccgacca tctgttctgg ccgtacccag ctgtcaaact
    15601 cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat
    15661 gcgctttttt ggcgatctcc gcggctttgt cataaccaat gtgggtgtta agcgcagtca
    15721 ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt tcaataccca
    15781 ctgcgcagtg tttgttaaaa ctttccatgc catctgccag caagcgcacc gattgcagga
    15841 aattgtggat caccattgga cggaagacgt tcagttcaaa gttaccggaa gcgcccccca
    15901 tgttgatcgc cacgtcgttc cccatcacct gacagcagag catggttaat gcctcacact
    15961 gtgttgggtt cactttcccc ggcatgattg agctgcccgg ctcattttcc gggattgaga
    16021 tttcaccaat tccgcagcgc gggccagagg ccagccagcg gacatcattg gcgattttca
    16081 tcagtgacgc agccaaccct ttcaacgcgc cgtgcgcctg aaccagggca tcacaggtcg
    16141 ccagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg actgccagtt
    16201 catctgctac gcgacgcgca tactccggat gggtatttag tccagtaccc accgctgtac
    16261 cgccaagagc cagttccgct acgtgaggca ggctgtattc gatatgtttg agattatgct
    16321 cgagcatcgc tacccagccg gaaatctcct gccccagtgt tagcggggtg gcgtcctgca
    16381 agtgggttcg accgattttt acgatatcgg caaatgcacg cgatttttca ctcagcgtct
    16441 gtgtcagggt tttaagctgc ggaatgagtt gcttgcgcag cgccagcagc gccgcaacgt
    16501 gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt
    16561 gaactttacg ttccatcccg cgcacgccgc cgagtaattc actggcccgg ttagccagca
    16621 cttcattcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga
    16681 attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt
    16741 tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc ttggttagcg
    16801 ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg
    16861 agcgttgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgcc cccatcgaat
    16921 ctttttcgct gcgtactgta ttcatggcct actcctcacc tgattaattt tttctttctg
    16981 ttttgctttc gttaagtaac ttttagctca ctcattattt actatttgat aacaaatgtt
    17041 tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta tttcacacag
    17101 cgggtgcatt gtgtgagttg tatctgctgg aagaagtcat ttcctttatc atccacaagg
    17161 ataaacgccg ggaaatcttc cacttcaatt ttccagatgg cttccattcc cagttccgga
    17221 tattcaacac attccaggct cttaatactt ccctgcgcca atacagcggc cggaccaccg
    17281 atactgccaa ggtagaagcc accgtgtttt ttacaggcat ccgtcacctg ctggctgcgg
    17341 ttgcctttcg ccagcatgat catacttccg cccagcgctt gcagttgatc gacataagaa
    17401 tccatccgtc cggcggtcgt tgggccaaga gaaccggagg cataaccttc cggcgttttg
    17461 gccggacccg cgtagtaaat cggatgatct ttgatgtact gcggcagccc ttcaccgtta
    17521 tccatccgct ctttcagttt ggcgtgagca atatcacgac cgacgataat cgtgccgtta
    17581 agcgataagc gtgtagaaac gggatactgc gacaactgtg cgaggatctc tttcatcgga
    17641 cggttaaggt caacgcgcac cgcttcgcct tctcccgctt tgcgcagctc ttccgggata
    17701 tatttgcctg gattatgttc cagtttttcg atccagatcc cctgacggtt gatcttcgct
    17761 ttgatattac ggtcagcaga acaggagacg cccataccga ccgggcagga tgcgccgtga
    17821 cgtggcaggc gaatcacgcg gatgtcgtga gcgaagtatt taccgccaaa ctgcgcaccc
    17881 agaccaagat tttgcgcttc gatcagcaat tctttttcca attccacatc gcggaacgcc
    17941 tgaccgtgct cattcccttc cgttggcagt tcatcatagt atttcgcgga agccagtttc
    18001 accgttttaa ggttcgtttc tgcagaagtt ccaccaataa cgaacgcaat atgatacgga
    18061 ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca ggtaattttt cagtttcccc
    18121 ggcgtcagta acgctttggt ttcctgatag agatacgtct tgtttgccga accaccacct
    18181 ttggcgatac agaggaattt gtattcgtcg ccatcaacgg cataaagatc gatctgcgct
    18241 ggcagattgg tgccggtatt cacttcttta tacatatcca gcggcgcgtt ttgcgagtag
    18301 cgcagattat cttcgatata agtgttatag acaccgcgcg ccagcgccgc ttcatcacca
    18361 ccaccggtcc atacacgctg ccctttttta ccaacaataa tcgcggtgcc ggtatcctga
    18421 caggttggca gaacgccttt cgccgcgatg tcggagttac gcaggaattg cagcgccaca
    18481 tatttatcat tttcgctggc ctccgggtca cgcagaatgt cggccacctg ttgttggtgc
    18541 gccggacgca gcatgaacga cgcatcatga aacgcctggc gcgccaacag agttaacgct
    18601 tcgggtgcga ctttcaaaat ctcctgccct tcaaattcag ataagctaac gtgttcgctg
    18661 gttagcaggt aatactcagt atcatctttt ttgagtggaa aaggagcctg ataatgaaag
    18721 ggtttgtttg acattgtttt ctcacttact gcctggtttg gttatgctct gggcgggtgt
    18781 tccgttgccc tgttaaaagc gagtaacaat atcctacgca cttttttaac aaaaactgag
    18841 actagtacga ctttttgcgg ctccaggtta cttcccgtag gattcttgct ttaatagcgg
    18901 gattaatttc cacattaaaa cagggattga tcatgcaaaa actcattaac tcagtgcaaa
    18961 actatgcctg gggcagcaaa acggcgttga ctgaacttta tggtatggaa aatccgtcca
    19021 gccagccgat ggccgagctg tggatgggcg cacatccgaa aagcagttca cgagtgcaga
    19081 atgccgccgg agatatcgtt tcactgcgtg atgtgattga gagtgataaa tcgactctgc
    19141 tcggagaggc cgttgccaaa cgctttggcg aactgccttt cctgttcaaa gtattatgcg
    19201 cagcacagcc actctccatt caggttcatc caaacaaaca caagtctgaa atcggttttg
    19261 ccaaagaaaa tgccgcaggt atcccgatgg atgccgccga gcgtaactat aaagatccta
    19321 accacaagcc ggagctggtt tttgcgctga cgcctttcct tgcgatgaac gcgtttcgtg
    19381 aattttccga gattgtctcc ctactccagc cggtcgcagg tgcacatccg gcgattgctc
    19441 actttttaca acagcctgat gccgaacgtt taagcgaact gttcgccagc ctgttgaata
    19501 tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa atcggcgctc gacagtcagc
    19561 agggtgaacc gtggcaaacg attcgtttaa tttctgaatt ttacccggat gacagcggcc
    19621 tgttctctcc gctgttgctg aatgtggtga aattaaatcc tggcgaagcg atgttcctgt
    19681 tcgctgaaac accgcacgct tacctgcaag gcgtggcgct ggaagtgatg gcaaactccg
    19741 ataacgtgct gcgtgcgggt ctgacgccta aatacattga tattccggaa ctggttgcca
    19801 atgtgaagtt cgaagccaaa ccggctaacc agttgttgac ccagccggtg aaacaaggtg
    19861 cagaactgga cttcccgatt cctgttgatg attttgcctt ctcgctgcat gaccttagtg
    19921 ataaagaaac caccattagc cagcagagtg ccgccatttt gttctgcgtc gaaggcgatg
    19981 caacgttgtg gaaaggttct cagcagttac agcttaaacc gggtgaatca gcgtttattg
    20041 ccgccaacga atcaccggtg actgtcaaag gccacggccg tttagcacgg gtttacaaca
    20101 agctgtaaga gcttactgaa aaaattaaca tctcttgcta agcttattaa aggcttataa
    20161 caccttcagg cggccagtcc gcctgatttc attttatgga taatcattat gaataaatcg
    20221 ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg cgcatggtat
    20281 acaggcaaga agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa cgcgcaactc
    20341 aaactgaccg ctcctgaatc caacctggaa gtgagttatc aaaactatca tcgcggcgta
    20401 ttcagcagtc agctgcaact gttggtgaaa cccattgccg gaaaagaaaa tccgtggatt
    20461 aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc atggtccctt cccccttgcc
    20521 cagcttaaaa aactgaacct gatcccgtcg atggcatcaa ttcaaaccac gctggttaat
    20581 aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt tgagattaac
    20641 tcgcgcattg gttacagcgg tgattccagt tccgatattt cgctcaagcc actaaattac
    20701 gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa tgcggacaga
    20761 gacggcaaag ctatctccct ttccggggag gcgcaaagtg gtcggataga cgcggttaac
    20821 gaatacaacc agaaagtaca gttgaccttt aataatctga aaaccgacgg ttccagcacg
    20881 ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt cactggaaaa aatgaccatt
    20941 tcagtggaag gcaaagaact ggcactgctg gaaggcatgg agatcagcgg taaatcggat
    21001 ctggtcaatg acggtaaaac gatcaatagc caactggatt actcgctaaa cagcctgaag
    21061 gtacagaatc aggatctggg cagcggcaag ctgactttaa aagtcggcca aattgatggc
    21121 gaagcctggc atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct gctggcacag
    21181 ccagaaattg ccaacaatcc cgaactttat caggagaaag tgacggaagc cttctttagc
    21241 gccctgccgc tgatgttgaa aggcgatccg gtgattacta tcgcgccgct aagctggaaa
    21301 aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc ggcaacgact
    21361 aaagaagcgc cgcaaacgct ggcgcaggaa gtagatcgtt cggttaaatc tctggatgcg
    21421 aaactgacca ttccggtgga tatggcaact gagttgatga ctcaggtagc gaagctggaa
    21481 ggttatcagg aagatcaagc gaaaaaactg gcgaaacagc aagttgaagg tgcatcagca
    21541 atggggcaga tgttccgtct gaccaccttg caggacaata ccatcaccac cagcctgcaa
    21601 tatactaacg gtcagataac gttaaacggg cagaaaatgc cactggaaga tttcgttggt
    21661 atgtttgcaa tgccggcatt aaatgttccg gtcgtacccg ctattccgca gcagtaattc
    21721 acagccaaaa cgccggagat ttttctctcc ggcgttattt tttacttcag cataaaatca
    21781 tactgaatac tcattttcac atcccagtaa ttcaggcgat cgcccagcgt actgccatcg
    21841 cgcttttccc gccctttatc ggcagcaaga aagaaattca tcccttgtaa cggcccttcg
    21901 ctgacccgat agttcatacc acctaagata cccgcgcctt caatccgcac atctcgttta
    21961 ctggattcat agccgcgagt cacaatcggt gcaaatgtca gtgtaaattg ggagttaaac
    22021 gatagttgac gcccaattgc caggactgca ttccttcacg gttacgatca agacttaatg
    22081 aaaagggata gccaatgtcg gtatccacca cggcaccatt cattgcatgg cgcaaataac
    22141 caccactgcc aaatacgctt ccccacggcg cattccaggt caattgtcca ctaaccaacg
    22201 ccgtttcatt gggctggctg gtattacgac ttaaaccttc gagttgcgcg taaaagccga
    22261 gcaactcccc tttcaacgta taattcgggt cgagcggtat gcttttctcg agaatacttt
    22321 gtatctgatt ggaaacatct ttctgtaccg atgcccccag agaatagcga ctggtatcat
    22381 catgccaact ggcggctaaa aaagaacgag cgcgatgttt atcgtatttt cccgtctggc
    22441 tgttatacac cccttcatca cgaataccgg ttgttgaatc agtccataaa aatactcgtg
    22501 aacgccacat tccccacaga tcaatatttg gcgttaaagc ccaataacct aatgctcctt
    22561 cagaaactgt cggtaatgta cgttgcgaac tgccagccat taacggaata cgataaggta
    22621 agctaccata actataatcc gtcccgaagc gacctaattt aagcaggcta ttttcactgg
    22681 gttttatttt tactgccccg agggcaaagc caaaactttt atcaccatca agataccacc
    22741 gggtactcat atcagcacga gcacctaatt tataaacata atacgctccg ccttcaacgc
    22801 caataatatt ggaataataa ccactattga aatcgagcaa tcctccctgt acccatgcgt
    22861 aaatatctct tcccgcgcca gcactgggct tatcagccct gcgcaattcg tttttcagtc
    22921 tgatgcgtag tcccgcttca tcgtcagcca gttgcgccgc gtaagcagag gtaaggccag
    22981 aggcagccgt caggcaaata acggccatgg ccactatttt tctcatagtt gcacctttga
    23041 tgttctgatt atttttattg aatattaatt agtgatatcg ctgattaatt gctgctgcac
    23101 ttttttacga ttatcaattt caaccacgat ttctttgaat tttttatccg tgagcggata
    23161 aaaccagata ataacgaatg ccagtagcat aaatccgcaa ggtactaagg caattgatgt
    23221 gcggatgccc ataattactt ccggcgtttg cacctgattg gcgatatatc cgcttaatcc
    23281 caaaataaag gcaggaattg aaccaccgat tgcctgaccg catttacggg taaatgagaa
    23341 tagtgaatag gtgagccctt caattcgcac gccggtcagg tattcaccgt attctacggt
    23401 atcagcttcc agcgcccaca tcacggtcat ggtaacgccc tgaccaattg aggcaatggc
    23461 taacgcaacc aacgccaccg gcagcgacca gacggaaacc cagaagaaca gcagataacc
    23521 gcaggttccc agcaaagcgc caatcaggaa ggtattcttt ttaccgatcc tcgcgaccat
    23581 ccccggcacc aacggtgccg atgccacagt accaaccagg ttttgcacca gtaccagcac
    23641 agtgaacagc ccggtatcat ttaacacata gcgcacgtag aacaacgacg aggcgctgac
    23701 cgcgaaggtc gaaatcagca cacacagcgc accgatgcac aacataaaca gcgggcgatt
    23761 ccgtttcagg gtttgcagac tgatcttcaa tgacggctgc gccacgatac gtaccacatt
    23821 ctcacgcgtc gatttgaagc agatgaagta aagcaccatt ccggcaatcg ccagcacaat
    23881 cgtccagaaa tggtataccg acaccatctc ttccgggctg gagttcttaa tgctcggccc
    23941 tatcagaaat gccaggcaga caaaggtcaa tgaagcggca atcccacgag ccgcgcccag
    24001 acgggcgcgg gattgtggtt gttgggtcat cgcggtagca agtgaaccat aaggaatatt
    24061 caccaggctg tagcaaagcc cgaggcccat gtaggtcaaa tatgcataca ccactttgct
    24121 accatggctc cagtcggtcg gcacccagaa taccagcacg ctgaagatca ttaacggcgc
    24181 agtaccgaag agtaaaaacg ggcggaattt tccccagcgg gtattcacac tgtccaccac
    24241 tcgtccggca aagacgtcgg cgaaggcatc gaatacccgc accagtaaca gcatggtgcc
    24301 cgccgcagcg gcaccgacgc cagcgacgtc ggtgtagtaa ctcaacagga agagcgcccc
    24361 cattgcgaag gcgaagttat tggcgatgtc accaaggctg tagccgacga tggtgcgcca
    24421 ggagagttgt tgattcattg tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca
    24481 gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg ggtgaagatc
    24541 cccttcttgt taccgccaac gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc
    24601 catacctgtt caccgacgac ggcgctgacg cgatcaaaga cgcggtgata catatccagc
    24661 catgcacact gatactcttc actccacatg tcggtgtaca ttgagtgcag cccggctaac
    24721 gtatccacgc cgtattcggt gatgataatc ggctgatgca gtttctcctg ccaggccaga
    24781 agttcttttt ccagtacctt ctctgccgtt tccaaatcgc cgctttggac ataccaaccg
    24841 taataacggt tcaggcacag cacatcaaag agatcgctga tggtatcggt gtgagcgtcg
    24901 cagaacatta cattgacgca ggtgatcgga cgcgtcggat cgagtttacg cgttgcttcc
    24961 gccagtggcg cgaaatattc ccgtgcacct tgcggacggg tatccggttc gttggcaata
    25021 ctccacatca ccacgcttgg gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt
    25081 aagtgcgcct gctgagtttc cccgttgact gcctcttcgc tgtacagttc tttcggcttg
    25141 ttgcccgctt cgaaaccaat gcctaaagag aggttaaagc cgacagctgc agtttcatca
    25201 atcaccacga tgccatgttc atctgcccag tcgagcatct cttcagcgta agggtaatgc
    25261 gaggtacggt aggagttggc cccaatccag tccattaatg cgtgatcgtg caccatcagc
    25321 acgttatcga atcctttgcc gcgcaaatcc gcatcttcat gacggccaaa gccagtaaag
    25381 tagaacggtt tgtggttgat caggaactgt tcgcccttca ctgccactga ccggatgccg
    25441 acgcgcagcg ggtagatatc acactctgtc cggcttttgg ctgtgacgta cagttcatag
    25501 agataacctt cacccggttg ccagaggtgc ggattcacca cttgcaaagt cccgctggtg
    25561 ccccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac
    25621 cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca
    25681 ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga
    25741 tcccggcgta gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg
    25801 taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca caaactgtga
    25861 tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc
    25921 cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg
    25981 catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct
    26041 gataccagac gttgcccaca taattacgaa tatctgcatc ggcgaactga tcgttaaaac
    26101 tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgctcaa
    26161 ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac
    26221 gggttggggt ttctacagga cgtaacataa gggactcctc attaagataa aaatactggt
    26281 caacctttaa tctgattagt taaccaattc tgtgttgcac acagttatct gtgagaaacc
    26341 catggaagag aaatggaaat aaaaagaact gacaccgcga tcaaaaaacc aaaaactgca
    26401 atacccacag cgtgatatag atcgcattaa tctttaaaac ggcgttgaca gaagccataa
    26461 aataagcaac cgaacgttcg ttcgagcatg ttgggaacga taaactctca tgacatatgg
    26521 ctacggtgaa tattttaggg aggaattccc cgctccggcg agattgtatt ttgtacaggg
    26581 aaattaaagt atgaaacgcg ggctcagttg tcaccagccc gcggaaagag agagagaatc
    26641 aggatgcggt taagataccg ccaatcatcg cgcgtaatcc ctgagcaaac gacgcttcac
    26701 gcgccaggtc ttccgcttcg atatcggcca gtacaccgta ggtcagatcc agcagtaaac
    26761 gtgaagtcat tgccgtgttg atgtctgggc ttatctcgcc tttttgctgg gcatcacgca
    26821 tccgctgagc aacaaattca gtgatcgtca tatgcttatt tttaagcatg gcggcgacct
    26881 gtgggttacg catcccttcc gccataattt cgaccaccag cgcccgttgc ccaaaggctt
    26941 catgggtgag agagacaata gactcgacca tatagtcaac aaaatgaatc ccttcaatcg
    27001 gttcccggaa acgggccagc gccctctcct ggtcctgtaa gataatggcc tgaatcaagg
    27061 cttctttgga gatgaaatgg tgatagagcg tcccgggact aatggcgcag gatttacaga
    27121 tggctttcat cgaggcactg tgaaatccat tttctgaaaa aatctctctg gcagcattga
    27181 ggatccgggt ccgtgtcggt tgtgcttcag tctgcatgtt atccatcatt aaaccattat
    27241 agttagcgtg aataactagt ttaccgcgtt gtcaggtgag cgcctatcat tgctgttgaa
    27301 tagtgcgaag gcacactcta ttcgtatgct tttcatccag ctattggtta accaatggcc
    27361 gtgatttggc tgccagttgt ttagcagaga cgttgatgaa ttgcacacgc tatcccggta
    27421 aacaacacat acggctgttt accggttcgt tagtgtatta attgagctcc tgtaccccac
    27481 caccggagac ggtgagaatt tgtccgctta cccagctggc tgcaggcgag caaaggaaca
    27541 gcgctgcgtt agcaatatct tgcggttggc ccagacgtct gatcggcgtg tgctgtaaca
    27601 ttttttgttc aatttctggt gtaataacgg atttcagggc atcggttaat attgcccccg
    27661 gcgcaatgcc attcacccga atattttttt cacccaggtc aaatgccata tttctgacca
    27721 gatgactggc cgcagcttta gatgatgcat aggaagtcat gtttatattt ttattttctg
    27781 ccgccataga agtgatggtc agaataacgc caccgccatt tttttccatt tctggcgcaa
    27841 caagttgtga cagatggaaa aaagaaaaca cattcagttc ataagcacgg cgaaaatccg
    27901 ccattggcat atcaaacggt ttaggtccac cgccaccggc gttgttaacc agaatatcaa
    27961 ctttacccag cttactgata gcaaagtctg ccagtgcaga gagttcctgt tcggaagtaa
    28021 tatcacaacg gcaggcaaat gcctgaccac ccagttgttg aatttcgtct acaacatggt
    28081 tagctgcgtc ggcgttaata tcactgacca ccacagatgc gccagctgtc gcgaatgtaa
    28141 tggcgatttc tttaccaata cctgcacccg cacctgtgat gatggcgcat tttccgtcga
    28201 gtctcaggtt gtcagaatta aacacataaa cctcctgtga acttcagtaa aaataaaagg
    28261 caccgcctgt gtgccacatt ttttatcatt aaacttgagg aaaggacact accaggtata
    28321 gcagataaag agaggattat ttcgccgcaa tgagccgggc gggaataata agattgcgtg
    28381 aatgcgtctc ttcatgggtg attttttgca tcatgcgatc cgcaagtgtg gtaccaagtt
    28441 cccgcgcagg cgtgctggcc caggtaacgg gtatatcatc aagtgtggtt ggtgtcgcat
    28501 cggtaaatgc cgccagcgaa acctgttgct caaagtaacg atcgactccg ctttccccgc
    28561 tttgccgccc tgctttcagc aaaccaaacc atgcccccat cgcaatagtt tcgttatagc
    28621 aaaccacggc actgatggtc gggttatgac gtaaaagcgc cgtgatagct tccgcggctt
    28681 gcttctggct ggaagtgcac tccaacaccc aatcgctgtg aaacggcagg ccaaatttta
    28741 gtagagttgc acaatagccc cccacccgtt ctgcacgggt taatgaggaa ctttgccctc
    28801 ccagccaggc gatccgctga tgcccattgc gaatgagatg ctccgtcaac aactgtgcag
    28861 cctgcatgtt gtccgggcga accgtatcaa catcatcaag ataactggca cgggaagcga
    28921 aaatcaccgg gatagctttt tcttctgcca tccgtcgcag gtcatcgctg cttcctgcag
    28981 ccccggcaat taccacacca tcgacaccct gattcagtaa cagtgaaaac cgctgtgcca
    29041 gctgctcgcc gtctttaccg ccgtgaagca aaaaaaccat ccgtccctgc gcttccagag
    29101 cttccgtcaa tccggccgtc aattcggcgt aaaacggcgc agataaatca cggacgatca
    29161 aaccaatgac gccgctttgc ccaccgcgca gcgccgacgc ctggcgattg cgcacaaatc
    29221 ccagctcttc aatggcggcg ttaacgcgtt ctcctgtggc ggtagagatt cgccctttgc
    29281 cactaagcac cagcgaaacg gtacttaccg acacgcccgc agccagcgca acatcatgaa
    29341 tggttatttt tttggcggta gccatgcaga tgacctactc cctgattttg tgacagataa
    29401 aacgttttac cttttattta tcttataccc gctattatcg ttgcgtaatg tgatttatgc
    29461 ctcactaaaa tttgataaaa cgttttatct tctcgcgcaa tttactgaat ccagattgtt
    29521 ctctacgagg agtcgtttta tgacggcgaa aacagcaccg aaagtcacgc tgtgggagtt
    29581 cttccagcag ttaggcaaaa ccttcatgtt acccgtggca ttattgtcgt tctgcggcat
    29641 tatgctcggc attggtagtt cccttagtag ccacgatgtc atcaccctgc tcccggtcct
    29701 gggcaacccc gtgttgcagg ctatctttac ctggatgagt aagattggct cgtttgcttt
    29761 tagtttcctg ccagtcatgt tctgtatcgc catcccgctg ggtctggcac gagaaaacaa
    29821 aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta atgaacctcg cggtaaactt
    29881 ctggttgacc aataaaggca ttctgccaac cacggatgcc gccgttctga aagccaataa
    29941 catccagagc attcttggga tccagtcgat cgacaccggg atcctcggtg cggtgatcgc
    30001 cggtattatc gtctggatgc tgcatgagcg attccataat atccgcctgc cggatgcgct
    30061 ggcattcttc ggcggtacgc gcttcgtacc aatcatctcc tcgctggtga tgggccttgt
    30121 cggcctggtg attccattag tctggccgat tttcgccatg ggtattagcg gcttaggcca
    30181 tatgatcaat agcgcgggtg atttcggacc gatgctgttt ggtaccggtg aacgtctgct
    30241 gttgccgttt ggtctgcatc acattctggt ggcattaatt cgctttaccg acgcaggtgg
    30301 cacgcaggaa gtctgcggtc aaaccgtcag cggcgcattg accatcttcc aggcgcaatt
    30361 gagttgcccg accactcacg gtttttctga aagcgccacg cgtttccttt cgcaaggcaa
    30421 aatgcctgcg tttctcggcg gtctgccagg tgcagcgtta gcgatgtatc actgcgcgcg
    30481 cccggaaaat cgccataaaa ttaaagggct gctgatttct ggcctgatcg cctgtgtcgt
    30541 tggcggcacg accgaaccgc tggaattcct gttcctgttc gtagcgccag ttctgtatgt
    30601 catccacgcg ctgttaaccg gcctcggctt caccgtcatg tctgtgctcg gcgtcaccat
    30661 cggtaatacc gacggcaata tcatcgactt cgtggtgttc ggtattttgc atggtctgtc
    30721 aaccaagtgg tacatggtgc cagtggtggt ggcaatctgg tttgtcgttt actacgtcat
    30781 cttccgtttc gctatcaccc gcttcaatct gaaaaccccg gggcgcgata gcgaagtggc
    30841 cagttcaatc gaaaaagccg ttgccggtgc gccgggtaaa tcaggttaca acgttccggc
    30901 aatcctcgaa gccttaggcg gtgccgacaa tattgttagc ctcgataact gcattacccg
    30961 tctgcgtttg tctgtgaaag atatgtcgct tgttaatgtg caggcactga aggacaatcg
    31021 ggcaattggc gtagtacaac ttaatcaaca taacctgcag gttgttatcg ggccacaagt
    31081 tcagtcagta aaagatgaaa tggccggtct gatgcatact gtccaggcat aaggataaga
    31141 tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca tggtgtacgc agtgggatta
    31201 tgtcgctgac cgtttcggca ctgctgacct gttacctttc acgatttcag acatggattt
    31261 tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc ctgatgcacg gcgtgtttgg
    31321 ctacagccgc tggaagaacg atgagtttct cgcggctatt gcccactggt tttccaccca
    31381 gcattacacc gccatcgatc ctcagtcggt ggtgtatggc ccttctgtta tctatatggt
    31441 ctcagaactg attcgtcagt ggtctgaaac aggtgaaggc gtggtgatcc acacacccgc
    31501 ctatgacgca ttttacaagg ccattgaagg taatcagcgc acagtaatgc ccgttgcttt
    31561 agagaagcag gctgacggct ggttttgcga tatgggcaag ctggaagccg tgttggcgaa
    31621 accagaatgt aaaattatgc tcctgtgtag cccacagaat cctaccggaa aagtgtggac
    31681 gtgcgatgag ctggagatca tggctgacct gtgcgagcgt catggtgtgc gggttatttc
    31741 cgatgaaatc catatggata tggtttgggg cgagcagccg catattccct ggagtaatgt
    31801 ggcgcgggga gactgggcgt tgctgacgtc gggatcgaaa agtttcaata ttcccgcgct
    31861 gaccggtgct tacgggatta tagaaaatag cagcagccgt gatgcctatt tatcggcact
    31921 gaaaggacgt gatgggcttt cttccccttc ggtactggcg ttaactgccc atatcgccgc
    31981 ctatcagcaa ggtgcaccgt ggctggatgc cttacgcgtc tatctgaaag ataacctgac
    32041 gtatatcgca gataaaatga acgccgcgtt tcctgaactc aactggcaga tcccgcaatc
    32101 cacttatctg gcctggcttg atttacgtcc gttgaatatt gacgacaacg cgttgcaaaa
    32161 agcacttatc gaacaagaaa aagtcgcgat catgccgggg tatacctacg gtgaagaagg
    32221 tcgtggtttt gtccgactca atgccggctg cccacgttcg aaactggaaa aaggtgtggc
    32281 tggattaatt aacgccatcc gcgctgttcg ctaaccccaa ttgcgcaacg taaaaaatcg
    32341 ttgcgcaatc gtggattttt accctgcttt gtttttataa tggtgcgcac ttttatatcc
    32401 agaaaaagag tgcgaccatg attgatacca ccctgccatt aactgatatc catcgccacc
    32461 ttgatggcaa cattcgtccc cagaccattc ttgaacttgg ccgccagtat aatatctcgc
    32521 ttcctgcaca atccctggaa acactgattc cccacgttca ggtcattgcc aacgaacccg
    32581 atctggtgag ctttctgacc aaacttgact ggggcgttaa agttctcgcc tctcttgatg
    32641 cttgtcgccg cgtggcattt gaaaacattg aagatgcagc ccgtaacggc ctgcactatg
    32701 tcgagctgcg tttttcacca ggctacatgg caatggcaca tcagctgcct gttgcaggtg
    32761 ttgttgaagc ggtgatcgat ggcgtacgtg aaggttgccg cacctttggt gtgcaggcga
    32821 agcttatcgg cattatgagc cgaaccttcg gcgaagccgc ctgtcagcaa gagctggagg
    32881 cctttttagc ccaccgtgac cagattaccg cacttgattt agccggtgat gaacttggtt
    32941 tcccgggaag tctgtttctt tctcacttca accgcgcgcg tgatgcgggc tggcatatta
    33001 ccgtccatgc aggcgaagct gccgggccgg aaagcatctg gcaggcgatt cgtgaactgg
    33061 gggcagagcg cattggacat ggcgtaaaag ccattgaaga tcgggcgctg atggattttc
    33121 tcgccgagca acaaattggt attgaatcct gtctgacctc caatattcag accagcaccg
    33181 tggcagagct ggctgcacat ccgctgaaaa cgttccttga gcatggcatt cgtgccagca
    33241 ttaacactga cgatcccggc gtacagggag tggatatcat tcacgaatat accgttgccg
    33301 cgccagctgc tgggttatcc cgcgagcaaa tccgccaggc acagattaat ggtctggaaa
    33361 tggctttcct caacgcagag gaaaaacgcg cactgcgaga aaaagtcgca gcgaagtaac
    33421 aaaaatggat ggtgcaaatt gcaccatcca tttttcatgc aaggcacaaa gtcgcgcgat
    33481 gtttggcgga ttcgatgccc agctcaatca actccattac ctggattgcc tggcttgccg
    33541 gaaccggatt ttcaccatcg ccatttaacg catcacgaat cgccgcatag taagccggat
    33601 aattcccagg caccgtcaac agcgtttctt cgacacgttc ctcaccttcc acgcgggtaa
    33661 gtacgccatc acgcatatcg tagccccagt cttcctgcgg cagacgctcg ccatttttca
    33721 gacgttcttc ctgtggatcg aggccatatt tcacataact gcctcgggat ccatgcacga
    33781 tataccgtgc tgactcagcg gctgccagca tggtaccgtg taaaatgact cgccgctgtg
    33841 gataggacaa gatggcgtgg aaataatcgg tcgactgcgc tccgggccgt aactgtgcca
    33901 aatctaccgt catgctgacc ggtaaaccaa atagcgtaat ggcctgatca agaagatgtg
    33961 gtgctaaatc gtaccagata ccgctgcctg ggccgccctg ttcacgccaa cgatcgcgca
    34021 cctgcggacg gaagcggtca aaatgagact caaagtaagc aacttcaccc agcacgcctt
    34081 ccgcgagcaa accttttagc gtcaagaaat cgctatccca acgacggtta tggaatacag
    34141 acagaacacg ccccaggctt tttgccagcg catccagctc tcgcgcttgt gacagtgtca
    34201 cggtaaaggg tttatcaacg accacatgtt tacccgcctc aagcgctgct ttggctaacg
    34261 ggaaatgggt atcgttgggt gtaggaatga caatcaggtc tatgttggga tcgttaaaca
    34321 gatgcttcgg ctcagagaca accgtaaccg ttggccagtc ggcttttact tttgtttcat
    34381 cactgctgga gattaccgcc agttccagcc cgggtgtgcc cgcaatcagg ggcgcatgga
    34441 aggttttgct cgcataacca tacccaatca acccaacacg gatgttgtcg ctcatgtcat
    34501 tgcctctcat tgaagtatga tggctatttg acactatcct ttaccctcgc tcaacagttt
    34561 aataacctgc cagcaataag ggatgttgtt taacttaagt caaaaaaata gcgatttttc
    34621 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
    34681 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa cagagatgtg agttgtaatg
    34741 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
    34801 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtactacggt acaacacaag
    34861 taagccctgc gcttgtagag cgacatgctg cccgtcaggg tgcatgtttt cacttgtcgg
    34921 atattaaacc aggaatttat tatcttgttc ggtgttgttg gtgattgtca gggatagtaa
    34981 agcgcgttac tcttttcata gcagtttttc atccctgaat atttgccatc agcgcattct
    35041 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac taataatacc
    35101 tacccctttg gtattaatta ttttatggac cttttatgac tgttcaggac tacttattaa
    35161 aatttcgcaa aatcagttca ctcgaaagtc tggaaaaact ctacgaccat cttaattaca
    35221 ccctgacgga cgatcaggaa ctgatcaata tgtatcgtgc tgccgatcac cgtcgcgcag
    35281 agctggtttc tggcgggcgt ttgtttgacc tcggccaggt accgaagtcc gtctggcact
    35341 atgttcaata aagaaagtag cgatcggtta taaaagcctt ataataacgc ccctaaaatt
    35401 agatttgcct gtttgcgtcg ctattctgga gaacctatga ccaccacaac gccacaacgt
    35461 attggaggct ggttgcttgg cccgttggcc tggcttttag tcgctctgtt aagtacgacg
    35521 ctggcgttgt tgctgtatac cgctgcgtta tcttctccgc aaacatttca aacgctcggc
    35581 gcacaagcac tgacgacaca aatcttatgg ggcgtttctt ttattaccgc tatcgccatg
    35641 tggtattaca cactgtggtt gaccatcgct ttctttaaac gtcggcgttg tgttcctaaa
    35701 cactatatta tctggctgct tatttccgta ttgctggcgg taaaagcgtt cgctttttca
    35761 cccgtggaag atggcatagc cgttcgccag ttgctgttta ctttgttggc aaccgcattg
    35821 atcgtcccct atttcaaacg ctcgtcgcgg gtgaaagcga cgtttgtgaa tccgtaataa
    35881 ccttacagtt aacctgttgt cgcctgctct ggattaacgg ataataggcg gcttttttat
    35941 ttcaggccga aaaatgactg actacctgtt actgtttgtc ggaactgtac tggtcaataa
    36001 ctttgtactg gtcaagtttc tcggtctctg tccgtttatg ggggtttcca aaaagctgga
    36061 aaccgcgatg ggcatggggc tggcaacaac gtttgtgatg acgctggcgt ctatttgcgc
    36121 ctggcttatc gatacgtgga ttttgatccc acttaatctg atttacctgc gcaccctggc
    36181 atttattctg gtgattgctg tggtcgtgca gttcaccgag atggtggtgc gcaaaaccag
    36241 cccggtgctt taccgtttgc tggggatttt tttgccgctt atcaccacca actgtgccgt
    36301 gctcggcgtg gcgttgctga atatcaatct cgggcacaat ttcttgcagt cggcgctgta
    36361 cggtttttcc gccgctgtcg gtttttcgct ggtgatggtt ctcttcgcag ccatccgcga
    36421 acgccttgct gtggctgatg tcccggcacc ttttcgcggt aatgccattg cgttaattac
    36481 cgcaggtctt atgtctctgg cctttatggg ctttagtggt ttggtgaagt tgtaatgaat
    36541 gctatctgga ttgccgttgc cgccgtgagc ctgctgggcc tggcgtttgg cgccattctg
    36601 ggttatgcct cccgccgttt tgcggtggaa gacgatccgg tcgttgagaa aattgacgaa
    36661 atcttaccgc agagccagtg tggtcagtgc ggttatcccg gctgtcgccc ctacgcggaa
    36721 gccatcagct gtaacggtga aaaaatcaac cgttgcgccc ctggtggcga agctgtgatg
    36781 ctaaaaattg ccgagttgct taatgtcgag ccgcagccgc tggatggcga agcgcaagag
    36841 ctaacgcctg cgcggatggt ggcggttatt gatgaaaata actgtattgg ctgcactaaa
    36901 tgtattcagg cgtgtccggt agacgccatc gttggcgcta cccgtgccat gcatacggta
    36961 atgagtgatc tctgtacggg ctgcaattta tgtgttgatc cgtgcccgac gcactgcatc
    37021 tcgttgcaac cggtcgcaga aacacctgac tcctggaaat gggatctgaa caccattccc
    37081 gtgcgtatca ttcccgtgga acaccatgct taagttattc tctgcattca gaaaaaataa
    37141 aatctgggat ttcaacggcg gcatccatcc accggagatg aaaacccagt ccaacggtac
    37201 acccctgcgc caggtaccac tggcgcagcg ttttgtcatt ccactgaaac agcatattgg
    37261 cgctgaaggt gagttgtgcg ttagcgtcgg cgataaagta ttgcgcggcc agccgcttac
    37321 ccgtggtcgc ggcaaaatgc tgcctgttca cgcgcccacc tcgggtaccg ttacggctat
    37381 tgcgccccac tctacggctc atccttcagc tttagctgaa ttaagcgtga ttattgatgc
    37441 cgatggtgaa gactgctgga tcccacgaga cggctgggcc gattatcgct ctcgccgtcg
    37501 cgaagagtta atcgagcgca ttcatcagtt tggtgttgcc gggctgggtg gtgcaggatt
    37561 cccgacaggc gttaaattgc agggcggcgg agataagatt gagacgttga ttatcaacgc
    37621 tgctgagtgc gagccataca ttaccgccga tgaccgtttg atgcaggatt gcgcggctca
    37681 ggtcgtagag ggtattcgca ttcttgcgca tattctgcag ccacgcgaaa ttcttatcgg
    37741 cattgaagat aacaaaccgc aggcgatttc catgctgcgc gcggtgctgg cggactctca
    37801 cgatatttct atgcgggtga ttccaaccaa atatccttct ggcggtgcta aacaattaac
    37861 ctacattctg accgggaagc aggttccaca tggcgggcgt tcatccgata tcggcgtatt
    37921 aatgcaaaac gtcggcactg cttatgcagt gaaacgtgcc gttattgatg gcgagccgat
    37981 taccgagcgt gtcgtaaccc tgactggcga agcaatcgct cgcccgggca acgtctgggc
    38041 acggctgggg acgccagtgc gtcatttatt gaatgacgcc ggattctgcc cctctgccga
    38101 tcaaatggtg attatgggcg gcccgctaat gggctttacc ttgccatggc tggatgtccc
    38161 ggtcgtaaag attaccaact gtctgttggc tccctctgcc aatgagcttg gcgaaccaca
    38221 ggaagaacaa agctgcatcc ggtgtagcgc ctgtgctgac gcctgccctg cggatctttt
    38281 gccgcaacag ttgtactggt tcagcaaagg tcagcaacac gataaagcta ccacgcataa
    38341 cattgccgac tgcattgaat gtggggcttg cgcgtgggtt tgcccgagca atattcccct
    38401 ggtgcaatat ttccgtcagg aaaaagctga aattgcggct attcgtcagg aagaaaagcg
    38461 cgccgcagaa gccaaagcgc gtttcgaagc gcgccaggct cgtctggagc gcgaaaaagc
    38521 ggctcgcctt gaacgacata agagcgcagc cgttcaacct gcagccaaag ataaagatgc
    38581 gattgctgcc gctctggcgc gggtgaaaga gaaacaggcc caggctacac agcctattgt
    38641 gattaaagcg ggcgaacgcc cggataacag tgcaattatt gcagcacggg aagcccgtaa
    38701 agcgcaagcc agagcgaaac aggcagaact gcagcaaact aacgacgcag caaccgttac
    38761 tgatccacgt aaaactgccg ttgaagcagc tatcgcccgc gccaaagcgc gcaagctgga
    38821 acagcaacag gcaaatgcgg aaccagaaca acaggtcgat ccgcgcaaag ctgccgtcga
    38881 agccgctatt gcccgtgcca aagcacgcaa gctggaacag caacaggcta atgcggaacc
    38941 agaacaacag gtcgatccgc gcaaagccgc cgtcgaagcc gctatcgccc gcgccaaagc
    39001 gcgcaagctg gaacagcaac aggctaatgc ggaaccagaa gaaccggtcg atccgcgcaa
    39061 agccgccgtc gaagccgcta ttgcccgtgc caaagcacgc aagctggaac agcaacaggc
    39121 taatgcggaa ccagaagaac cggtcgatcc gcgcaaagcc gccgtcgaag ccgctattac
    39181 ccgtgccaaa gcgcgcaaac tggaacagca acaggctaat gcggagccag aagaacaggt
    39241 tgatccgcgc aaagctgccg tcgcagcggc tattgcacgc gccaaagcac gcaagctgga
    39301 acagcaacag gctaatgcag aaccagaaga acaggttgat ccgcgcaaag cggcagttgc
    39361 cgcggctatt gcccgcgttc aggccaaaaa agccgtccag caaaaggttg taaacgagga
    39421 ctaaatggta ttcagaatag ctagctcccc ttatacccat aaccagcgcc agacatcgcg
    39481 cattatgctg ttggtgttgc tcgcagccgt gccaggaatc gcagcgcaac tgtggttttt
    39541 tggttggggt actctcgttc agatcctgtt ggcatcggtt agtgctctgt tagccgaagc
    39601 tctcgtactc aaactacgca agcagtcggt agccgccacg ttgaaagata actcagcatt
    39661 gctgacaggc ttattgctgg cggtaagtat tccccccctc gcgccatggt ggatggtcgt
    39721 gctgggtacg gtgtttgcgg ttatcatcgc taaacagttg tatggcggtc tggggcaaaa
    39781 cccgtttaat ccggcaatga ttggttatgt ggtcttactg atctccttcc cggtgcagat
    39841 gaccagctgg ttaccgccac atgaaattgc ggtcaacatc cctggtttta tcgacgccat
    39901 acaggttatt ttcagcgggc ataccgccag tggtggtgat atgaacacac tacgcttagg
    39961 tattgatggc attagtcagg cgacaccgct ggatacattt aaaacctctg tccgtgccgg
    40021 tcattcggtt gaacagatta tgcaatatcc gatctacagc ggtattctgg cgggcgctgg
    40081 ttggcaatgg gtaaatctcg cctggctggc tggcggcgta tggttgctat ggcagaaagc
    40141 gattcgctgg catattcccc tcagcttctt agtaacgctg gcgttatgcg caacgttggg
    40201 ctggttgttc tcaccagaaa cactggcagc accgcaaatt catctgctgt ctggagcgac
    40261 catgctcggc gcattcttta ttttgactga cccggttacc gcttctacga ccaatcgtgg
    40321 tcgtcttatt ttcggcgcgc ttgcgggctt attagtctgg ttgatccgca gtttcggcgg
    40381 ctatcctgac ggcgtggctt ttgccgtcct gctggcgaac atcacggttc ctctgatcga
    40441 ttactacacg cgtccgcgcg tctacggcca tcgcaaaggg taaaccatgc tgaaaactat
    40501 ccgaaaacac ggcattacgt tggcgctatt tgcagcgggt tcaacagggt taactgcggc
    40561 catcaaccag atgaccaaaa cgacgattgc tgaacaggcc agtctgcaac aaaaggcttt
    40621 atttgatcag gtgctgccag ccgaacgcta taacaatgcg ctggcacaga gttgctatct
    40681 ggtaactgcg ccagagttag gtaaaggtga gcatcgggtt tacatcgcca aacaggatga
    40741 caaaccggta gccgccgttc tggaagcaac cgcgccagat ggctattccg gtgcgattca
    40801 gctgctggtg ggagccgatt ttaacggcac ggtacttggc acgcgcgtga cagagcacca
    40861 cgaaacgcca gggcttggcg ataaaatcga actgcgcctt tctgactgga tcacccattt
    40921 tgcgggtaaa aaaatcagtg gtgcggatga tgcgcactgg gcggtgaaga aagatggtgg
    40981 taatttcgac cagttcaccg gcgcgacgat tactccccgc gcggtggtta atgcggtaaa
    41041 acgcgccgga ttgtacgctc agacgttacc ggaacaactt tctcaacttc ctgcctgtgg
    41101 agaataaagc gtgagcgaaa ttaaagacgt tattgttcag gggttgtgga aaaacaactc
    41161 tgcgctggtc cagttgctcg gcctttgtcc tctgttggct gtcacgtcta ctgccactaa
    41221 cgctctgggt ttaggacttg cgactacgct ggtactgacg ctgaccaacc tgaccatttc
    41281 gacgctgcgt cactggacgc cagccgagat ccgcattccc atttacgtga tgatcatcgc
    41341 ctcggtggtc agcgctgtac agatgctgat caacgcctac gcctttggcc tgtatcaatc
    41401 attagggatt tttattccgc tgattgtcac taactgtatc gttgtgggcc gcgctgaagc
    41461 cttcgctgcc aaaaaaggtc cggcgctttc ggcactggac ggcttttcaa ttggtatggg
    41521 cgcaacctgc gccatgttcg tgctgggttc actacgcgaa attatcggca atggcacatt
    41581 gtttgacggt gcagatgcgc tgttaggtag ctgggcaaaa gtattacgcg tggagatttt
    41641 ccacaccgac tcccctttcc tgctggcgat gctgccacca ggtgcattta ttggcctggg
    41701 actgatgctg gcaggaaaat acctgattga tgaaagaatg aaaaagcgcc gtactgaagc
    41761 agctgccgaa cgtgcattgc caaacggtga aacagggaat gtctgatgaa caaagcaaaa
    41821 cgcctggaga tcctcactcg cctgcgtgag aacaatcctc atcccaccac tgagcttaat
    41881 ttcagttcgc cttttgaatt gctgattgcc gtactgcttt ccgctcaggc aaccgatgtc
    41941 agtgttaata aggcgacggc gaaactctac ccggtggcga atacgcctgc agcgatgctt
    42001 gaactgggcg ttgaaggggt gaaaacctat atcaaaacga ttgggcttta taacagcaaa
    42061 gcagaaaata tcatcaaaac ctgccgtatc ttactggagc agcataatag cgaggttccg
    42121 gaagatcgtg ctgcgcttga agccctgccc ggcgtaggtc gtaaaacagc caacgtcgta
    42181 ttaaacactg cattcggctg gccgactatt gctgtcgaca cgcacatttt ccgcgtttgt
    42241 aatcgtactc aatttgcgcc ggggaaaaac gtcgaacagg tagaagaaaa gctactgaaa
    42301 gtggttccgg cagagtttaa agtcgactgc caccattggt tgatcctgca cgggcgttat
    42361 acctgcattg cccgcaagcc ccgctgtggc tcttgtatta ttgaagatct ttgtgaatac
    42421 aaagagaaag ttgacatctg aagaaaaggg gtaacaccga ttaccccatt gataaccttt
    42481 ctttatcctc ttttaaaaca tctttttaac gtcaatgatg ccattgctta gcgttatcat
    42541 caggtaatcc g
//
LOCUS       DAZKFZ010000015        42303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000015 DAZKFZ010000000
VERSION     DAZKFZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            154..1374
                     /locus_tag="ACHOQW_000785"
     CDS             154..1374
                     /locus_tag="ACHOQW_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9571910.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1347..1976
                     /locus_tag="ACHOQW_000786"
     CDS             1347..1976
                     /locus_tag="ACHOQW_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9571911.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1977..3137)
                     /gene="ybdL"
                     /locus_tag="ACHOQW_000787"
     CDS             complement(1977..3137)
                     /gene="ybdL"
                     /locus_tag="ACHOQW_000787"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP9571912.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQASAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3246..4334
                     /gene="hcxA"
                     /locus_tag="ACHOQW_000788"
     CDS             3246..4334
                     /gene="hcxA"
                     /locus_tag="ACHOQW_000788"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP9571913.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4344..4541)
                     /locus_tag="ACHOQW_000789"
     CDS             complement(4344..4541)
                     /locus_tag="ACHOQW_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9571914.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4723..6828)
                     /gene="cstA"
                     /locus_tag="ACHOQW_000790"
     CDS             complement(4723..6828)
                     /gene="cstA"
                     /locus_tag="ACHOQW_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP9571915.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(7009..7422)
                     /gene="entH"
                     /locus_tag="ACHOQW_000791"
     CDS             complement(7009..7422)
                     /gene="entH"
                     /locus_tag="ACHOQW_000791"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP9571916.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7425..8171)
                     /gene="entA"
                     /locus_tag="ACHOQW_000792"
     CDS             complement(7425..8171)
                     /gene="entA"
                     /locus_tag="ACHOQW_000792"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP9571917.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8171..9028)
                     /gene="entB"
                     /locus_tag="ACHOQW_000793"
     CDS             complement(8171..9028)
                     /gene="entB"
                     /locus_tag="ACHOQW_000793"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP9571918.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9042..10652)
                     /gene="entE"
                     /locus_tag="ACHOQW_000794"
     CDS             complement(9042..10652)
                     /gene="entE"
                     /locus_tag="ACHOQW_000794"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP9571919.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10662..11837)
                     /gene="entC"
                     /locus_tag="ACHOQW_000795"
     CDS             complement(10662..11837)
                     /gene="entC"
                     /locus_tag="ACHOQW_000795"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP9571920.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12026..12982
                     /gene="fepB"
                     /locus_tag="ACHOQW_000796"
     CDS             12026..12982
                     /gene="fepB"
                     /locus_tag="ACHOQW_000796"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9571921.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12986..14236)
                     /gene="entS"
                     /locus_tag="ACHOQW_000797"
     CDS             complement(12986..14236)
                     /gene="entS"
                     /locus_tag="ACHOQW_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP9571922.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14347..15351
                     /gene="fepD"
                     /locus_tag="ACHOQW_000798"
     CDS             14347..15351
                     /gene="fepD"
                     /locus_tag="ACHOQW_000798"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP9571923.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15348..16340
                     /gene="fepG"
                     /locus_tag="ACHOQW_000799"
     CDS             15348..16340
                     /gene="fepG"
                     /locus_tag="ACHOQW_000799"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP9571924.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16337..17152
                     /gene="fepC"
                     /locus_tag="ACHOQW_000800"
     CDS             16337..17152
                     /gene="fepC"
                     /locus_tag="ACHOQW_000800"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9571925.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17149..18282)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOQW_000801"
     CDS             complement(17149..18282)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOQW_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP9571926.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18498..22379)
                     /gene="entF"
                     /locus_tag="ACHOQW_000802"
     CDS             complement(18498..22379)
                     /gene="entF"
                     /locus_tag="ACHOQW_000802"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP9571927.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22376..22594)
                     /locus_tag="ACHOQW_000803"
     CDS             complement(22376..22594)
                     /locus_tag="ACHOQW_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFP9571928.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22597..23799)
                     /gene="fes"
                     /locus_tag="ACHOQW_000804"
     CDS             complement(22597..23799)
                     /gene="fes"
                     /locus_tag="ACHOQW_000804"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP9571929.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24042..26282
                     /gene="fepA"
                     /locus_tag="ACHOQW_000805"
     CDS             24042..26282
                     /gene="fepA"
                     /locus_tag="ACHOQW_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP9571930.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26457..27077
                     /gene="entD"
                     /locus_tag="ACHOQW_000806"
     CDS             26457..27077
                     /gene="entD"
                     /locus_tag="ACHOQW_000806"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP9571931.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27199..27351)
                     /gene="hokE"
                     /locus_tag="ACHOQW_000807"
     CDS             complement(27199..27351)
                     /gene="hokE"
                     /locus_tag="ACHOQW_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9571932.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27702..27854)
                     /gene="hokE"
                     /locus_tag="ACHOQW_000808"
     CDS             complement(27702..27854)
                     /gene="hokE"
                     /locus_tag="ACHOQW_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9571933.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            27823..28090
                     /locus_tag="ACHOQW_000809"
                     /pseudo
     CDS             27823..28090
                     /locus_tag="ACHOQW_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28293..29411
                     /locus_tag="ACHOQW_000810"
     CDS             28293..29411
                     /locus_tag="ACHOQW_000810"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP9571934.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29477..29725
                     /locus_tag="ACHOQW_000811"
     CDS             29477..29725
                     /locus_tag="ACHOQW_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP9571935.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29790..30158
                     /locus_tag="ACHOQW_000812"
     CDS             29790..30158
                     /locus_tag="ACHOQW_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9571936.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30252..30905
                     /gene="nfsB"
                     /locus_tag="ACHOQW_000813"
     CDS             30252..30905
                     /gene="nfsB"
                     /locus_tag="ACHOQW_000813"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP9571937.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31013..32260
                     /gene="ybdG"
                     /locus_tag="ACHOQW_000814"
     CDS             31013..32260
                     /gene="ybdG"
                     /locus_tag="ACHOQW_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP9571938.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32328..33704)
                     /gene="pheP"
                     /locus_tag="ACHOQW_000815"
     CDS             complement(32328..33704)
                     /gene="pheP"
                     /locus_tag="ACHOQW_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP9571939.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33806..36943)
                     /gene="cusA"
                     /locus_tag="ACHOQW_000816"
     CDS             complement(33806..36943)
                     /gene="cusA"
                     /locus_tag="ACHOQW_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP9571940.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(36955..38178)
                     /gene="cusB"
                     /locus_tag="ACHOQW_000817"
     CDS             complement(36955..38178)
                     /gene="cusB"
                     /locus_tag="ACHOQW_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP9571941.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38194..38526)
                     /gene="cusF"
                     /locus_tag="ACHOQW_000818"
     CDS             complement(38194..38526)
                     /gene="cusF"
                     /locus_tag="ACHOQW_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP9571942.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38550..39932)
                     /gene="cusC"
                     /locus_tag="ACHOQW_000819"
     CDS             complement(38550..39932)
                     /gene="cusC"
                     /locus_tag="ACHOQW_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP9571943.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40089..40772
                     /gene="cusR"
                     /locus_tag="ACHOQW_000820"
     CDS             40089..40772
                     /gene="cusR"
                     /locus_tag="ACHOQW_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP9571944.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40762..42210
                     /gene="cusS"
                     /locus_tag="ACHOQW_000821"
     CDS             40762..42210
                     /gene="cusS"
                     /locus_tag="ACHOQW_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP9571945.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT        10106 a        11728 c        10782 g         9687 t
ORIGIN      
        1 tttttgatat ataacctcag acagctacta taactttcac ctatttattc acagcgcgaa
       61 tgaaaaataa aacactctgc ttttcagtgt ttactttata attaacagtc ttatctcact
      121 gatcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat tcctcttccg
      181 ctcaatattc tggaagctgc acgagagcgt attacctgga cactcaacac cctaccccgc
      241 gtttgtgtct cattttctgg cggcaaagac tctggtttaa tgctgcatct gacagccgaa
      301 ctcgcccgac aaatgggcaa aaaaatctgc gttttgttta tcgactggga ggcgcaattc
      361 tcttgcacca ttaactatgt tcagtccctg cgcgagttgt acaccgatgt catcgaagag
      421 ttttactggg ttgcgctccc gcttacgacg caaaattccc tttcacaata ccaacccgaa
      481 tggcagtgct gggaacctga tgtcgaatgg gtacgtcagc ccccgcaaga tgcgataacc
      541 gatcctgact ttttctgctt ttaccagcca ggcatgacct tcgaacaatt tgtacgtgag
      601 tttgccgaat ggttttcaca aaaacgtccg gcggcgatga tgatcggcat ccgtgcggat
      661 gagtcctaca accgttttgt cgccatcgcc agtttaaata aacaacgttt tgccgacgat
      721 aaaccctgga ctacggccgc accaggcggt catagctggt acatttaccc catttacgac
      781 tggaaagtgg ctgatatctg gacctggtat gcaaatcatc aaagtctctg caacccactg
      841 tataacctaa tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg cgaacctttt
      901 ggcccggagc aacgacaagg attatggctc tatcacgtta tcgaaccgga tcgctgggct
      961 gctatgtgcg cacgagtcag cggggtaaaa agtggcggca tttacgccgg acatgataat
     1021 catttctatg ggcatcggaa aatcctcaag ccagaacatt tagactggca agaatatgca
     1081 ttattgctgc tcaatagcat gccggaaaaa acagctgagc attaccgcaa taaaatcgcc
     1141 atttatttgc actggtatca gaaaaaaggc atcgaggttc cacaaaccca gcaaggggac
     1201 attggcgcga aagatatccc ctcctggcgg cggatatgca aagttttact caataacgat
     1261 tactggtgtc gggcattatc atttagtcct acgaaagcga agaactatca gcgttataac
     1321 gaacggataa aaggaaaacg tcaggaatgg gggatactat gcaacaacga ttaacgcagg
     1381 atttaacgca atttctcgcc agcttgccgg aagatgatcg catcaacgcg attaatgaaa
     1441 tccgcatggc gatccatcag gttagcccct ttcgtgaaga gccagtggat tgcgttctgt
     1501 gggttaaaaa cagccaactt atgcctaatg attacaaccc aaacaacgtg gcaccacccg
     1561 aaaaaaaact gctgaaaaaa tccattgaaa tagatggttt tacccaaccc attgtggtca
     1621 cgcatacgga taaaaatgcc ctggaaatcg tggatggttt tcaccgccat gaaattggta
     1681 aaggctcatc atcattaaaa ttacgcttaa aaggttattt gccggtgacc tgtctggaag
     1741 gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa tcgcgctcgt ggacgccatc
     1801 aaatcaccgc catgtcggaa atcgtccgtg aactcagtca gttgggatgg gacgataata
     1861 aaattggcaa agagctggga atggacagcg acgaagtgct gcgcctgaag caaattaacg
     1921 gtctgcaaga gctgtttgcc gaccgtcaat attcccgcgc ctggactgtc aaataactac
     1981 agctggcgca gtcgttcagc tgctgccagc aacgtcgatt ccttcttggc aaaacagaga
     2041 cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg atagcggaat cgccgccacg
     2101 ccgtgctccc gcgtcagcca ctggcaaaac tcaacatcat ccagggtaga aaccgcgctg
     2161 taatccacca gcaaaaagta tgtgccttcg cacggtaaaa tctccagccg actttcattt
     2221 aaggcattta ccagaatatc gcgcttctgg cgataaaagt ccggtaacgc aagataatgc
     2281 tcaggttctg cacgtagcat atccgcaatt gccagctgtg ccggggtatt caccgaaaag
     2341 gtcagatact gatgtacctt gcgaatttcg gcgctgatgg gcgctggcgc aacacaataa
     2401 cccactttcc agccggtcat atgataggtc ttgccaaacg atgacaccgc caccgcccgc
     2461 tcacgcagct gcggatgcgc cagcacactg gcatggccct gttgtgaaaa gttgatgtgc
     2521 tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg cgctcgcctg ccacaaagcg
     2581 gcgaaatcag cctgctgcca gacagttgca ctgggattat gcggagtgtt gaggatcacc
     2641 agtctggtgc gctcgcttaa caatgcggca aattcctgcc agtcaacgcg aaaatgcggt
     2701 ggttgcagtg ccatacgctt cactattccc ccagaaagcg cgatggcggg ggcgtaactg
     2761 tcatagctgg gatcaaaaca aatcacttca tcgccattgc gcaccagtgc ggtaatcgcc
     2821 gcgtataacg cttccgtcgc ccctgccgtt acggtgatat cgctatcggc atctggttga
     2881 tagccataca aacgttccgt tttctgagca atcgcctcgc gcaaggcctg cacgccggtc
     2941 atgggcgcgt attggtttgc cccctgggca acgtggtacg ccagccgctc ctgtaaatag
     3001 cgcggaccat caaaatcagg aaagccttgc gacaggttaa tcgcctggtg ttgctgcgcc
     3061 agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg gaagtttgct ttgtggaatc
     3121 agagggttat ttgtcatttt tattgtacct gacgaagttg cgtgttgaag gcactataac
     3181 acgatgctag tatttggcaa tcaagacgtt tagatgtcta aataaaacaa taaggacaac
     3241 acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc ggctaactac ttttcacatc
     3301 caggaagttt caatcacctg cacgattttt tcactgatga acaactttct cgcgcggtgt
     3361 ggatctacgg cgtacgcgcc attgctgcgg cgcaaaccaa acttccgcca gcgtttggac
     3421 ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag cgaaagcgat gtacagcaac
     3481 tggcggctga gtccggtgac gatcgcagcg tggtgattgg cgtcggcggc ggcgcactac
     3541 tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc atttgttgcc gctccaacga
     3601 tcgccgccac ctgcgccgcc tggacaccgc tctccgtctg gtataacgat gccggacagg
     3661 cgctgcatta tgagattttc gacgacgcca attttatggt gctggtggaa ccggagatta
     3721 tcctcaatgc gccgcaacaa tatctgctgg cggggatcgg tgatacgctg gcgaaatggt
     3781 atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc gctaaccgtg cgactgggga
     3841 tcaataatgc tcaggccatt cgcgacgtct tgttaaacag tagcgaacag gcgcttgccg
     3901 atcagcaaaa tcagcagtta acgcaatcat tttgcgatgt ggtggatgcc attattgctg
     3961 gtggtgggat ggtcggtggt ctgggcgatc gttttacgcg tgtagcggcg gcccatgccg
     4021 tacataacgg tctgactgtc ctgccacaaa ccgagaagtt tctccacggc accaaagtcg
     4081 cctacggaat tctggtgcaa agcgccttgc tgggtcagga tgatgtgctg gcgcaattaa
     4141 ccggggcgta tcagcgtttt catctgccga ctacgctggc ggagctggaa gtggatatca
     4201 ataatcaggc ggagatcgat aaagtgattg cccacacctt gcgcccggtg gagtccattc
     4261 attacctgcc agtcacgctg acaccagata cgttgcgtgc agcgttcgaa aaagtggaat
     4321 cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc cgccttttcc accgtagcgc
     4381 gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg gcgtttgatc gggatggtta
     4441 acccgcatat gttcgacata gttgtcgtaa tcaggcatac caatcatcag cttcgccgcc
     4501 tgacctaaat attttccggc ttttgccagt gaatcaaaca tcttggcctc cgctaaccat
     4561 gttgctgaaa gagtgccgga tgcggcgtga acgccttatc cggcctacaa atgctatcaa
     4621 cgcgctcggt cagccccctc gccccatcgg ggagagggtt agggtgaggg ggaacccacg
     4681 gcgccatgcc aacattcgcc aacatccccc ctcactctga ctttagtgcg cgccttttgc
     4741 ctgcaccacg atctcctcga cattttccgg cattggctca tacggcgttt ctttcgccgt
     4801 tggcttcggc tctttcaatg ccgcaagtgc cgtcttaatc gagaacagtg ccagaaccac
     4861 cacgaccacc ataaagaaga tggttaaccc ggcatccaga cggttgttga acaccagttg
     4921 cgccagctgt gactcagtat actgcgatgg aatattaccg ctgtcgatca ttgcctggaa
     4981 cttattagca atggccagga agccgacttt cgcatccggg ctaaacgctt tctgccagcc
     5041 tgcggtcagg gtacaaatca gcagccaggc cgttggtacc agcgccaccc aggcgtaacg
     5101 ttgacgtttc atcttgaaca acaccacggc acagagcatc agcgccatcc ctgccagcat
     5161 ctggttggca ataccaaaca gcggccacag agtgttaata ccgcccagcg gatcgaccac
     5221 accctgatgc aggaagtagc cccacgccag cacgcacagc gccgttgcca gcaggttagc
     5281 aggcagtgaa tcggtccgtt tcaggcccgg agaaaccacg cccagcagat cctgcaacat
     5341 aaagcgcgca gcacgcgtac ctgcatccac cgccgtcaga ataaacagag cttcaaacag
     5401 aatggcgaag tgataccaga acgccacatc catcatgccg cccagcgcgc catgcagaat
     5461 gtaggccatc cccaccgcca acgtcggcgc accgcccgca cgggaaatga tcgactgttc
     5521 tcccacttcg ctggcaatct ggttgagcgt atccggagta atcgcaaagc cccagctact
     5581 caccacttgc gcggcagaag cgaccacatc cgccgtccct gccggagcca gcaccgccat
     5641 cgggctgttc atggcgaagt acacgcccgg atcgatgata caggcggaaa ccagtgccat
     5701 aatcgccacg aaggattcca ttaacattcc accgtagccg ataaaacaag cctgcccttc
     5761 gttcgccagc atcttcggcg tggtgccgga agagatcagc gcatggaagc cagacaccgc
     5821 gccacaggcg atggtgataa acaggaacgg gaacaggtta ccggtccata ccgggccagt
     5881 gccgtcaaca aatttggtca acgcaggcat ggtcagcgtc gggcgcataa tcaaaatgcc
     5941 taccgccaga ccaacgatcg tcccgatttt caggaaggta gagaggtagt cacgcggagc
     6001 cagcagcaac cataccggca gcaccgccgc cacaaaaccg taacccacca gcatccaggt
     6061 cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc ggactttctg ccacccagcc
     6121 gccagaaata atggcgaaaa tcaggaatac cagaccgatg accgacactt caccaatacg
     6181 ccccggacgc agatagcgca ggtagatccc catgaacagc gccagcggaa tggtgaacgc
     6241 aacggtatac gttccccacg ggctatgagt cagggctttc accacgatca tcgccagtac
     6301 tgcaaggata atgaccatga tcataaagca ggccaccagc gcgatcaccc cggcggttgg
     6361 ccccatctct tctttgacca gctcacccag cgaacgaccg tcacggcgcg tagaaacaaa
     6421 cagcaccatg aaatcctgca ccgcaccggc gagaaccacc ccggcgagta gccagatcat
     6481 ccctggcagg taacccattt gcgccgccag taccggccca accaacggac ctgctccggc
     6541 aatggccgca aaatggtgac cgaacagcac tttcttgtcc gtcggcacat agtccagccc
     6601 gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca accgccagca catttttggc
     6661 gatatacaga ccataaaaac ggtaagcgat cagatagata cagaccgacg ccaccacaat
     6721 ccacagcgcg ttgatctgtt ccccacgatt taaagcaata tatcccagag caaatgctcc
     6781 cattacagag agcactgtcc agacgaggta tttccctgat ttgttcatag ttgttatccg
     6841 tgtgcgtgtc catagagatg ttacattttg tttctataac atctttgtac gatttaacaa
     6901 cacagcaacc atgtaaatcg gagttgaaac caatatttaa cttaacaatg ttaactcgat
     6961 cactccgtta accgagtttt cctgcaatct catataattc tgtcgggttc atcccaaaac
     7021 tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt tcatcgaaaa cgacgatttc
     7081 ccagctctga ttttgccgcc caagatgcag cggctggcag acgccgcgta ccttgccttc
     7141 agacaccggg cgatgatgtg tggcgttaag ttccgtgccc accacacact gcccgtcgcg
     7201 ggtcatcata aatccggcca tcgatcccag cgtttcagcc agcgccgccg acgcgccgcc
     7261 atgcagcagg ccaaacggct gatgagtacg gatatcaacc ggcatttcgg cttccagcac
     7321 atcatcgccc agacgggtat acacaattcc cagatgcgcc accattgtgt tatcgctggt
     7381 ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc atgcttatgc ccccagcgtt
     7441 gagccgccat cgaccacaat atcctgtagg gtgatatggc tggcgaggtc agaggcgagg
     7501 aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt tccccagcgg aatgccgagt
     7561 ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg cgtcatcgct cacccacagc
     7621 gtgcgttgca tatcggtatc ggtggaaccc ggcgaaacca cattacagcg cacgccgcta
     7681 cccgccagtt ccagcccgac gctcaacgcc aggcttttca gcgccgcttt cgatgcgcca
     7741 taagcactca taccaatacg cggcgtgtgt gcggcgtcgg acgccacagt gacaatcgcc
     7801 ccgccccgct gacggcgaaa ctggttcatg gtttgctgga acaggttaaa cgcaccgccg
     7861 acattaaccg caaaagtctg ctgccagtcc tccttactga gctgatcggt cgcgcccatg
     7921 cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct ccgtttcagc taacagtcgc
     7981 tgacatacct gcgccacctg cgcagcatcg gcaacatcca ttacttcggt cgcaaaggga
     8041 tattgctcct gagcgaacgc ttgatcaaaa cctgtaactt tcgctcccgc ttcaacaaac
     8101 gccagcgccg tggcgtagcc gatacctttc cccgcgccgg ttacccagac atttttaccg
     8161 ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca ccaggcgtcg atggtcgggt
     8221 ttttcgccaa catgacaaag tcgatatcgc catgcacttt gcgccagcgc gccgccagcg
     8281 ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt gtcgtcatcg aacggttcat
     8341 cggactcgtc cagcaacggc aggatcacct cacgcagcgc cgctttgctg gcggggatag
     8401 gtgctggcag taattcttcg gtcatcacca cccggccaga acgtccggcc acatatttca
     8461 gcgacatcaa atgctcgtca cggctgaaat cggccagcgc atccgccacc ataaacggtt
     8521 taatatcgcg cataaatgcg tcggttgcgg tggtcataca gccaatgtgg gcatataccc
     8581 cggtaataat cagctggtta cgtccactct ctttgagcat ttgctccagc ggagaacgat
     8641 gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc ggcatctggc gtcagtcgat
     8701 ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg cccccacata tcattcaaca
     8761 gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt gtaataaacc gggatattgt
     8821 gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat cacctgctcc atcatcgggc
     8881 agttctcgcc ccagaagctg acaaaatagt cctgcatatc atggattaac aacgcggcac
     8941 gttgcggttc aaaggcccag tcaactttat tctgcggaat atcgtgagac tccggcagtg
     9001 cgtaagcctg taattttgga atagccatcg tgttctctcc ttcaggctga tgcgcgtgac
     9061 gccagccact gacgtaattg ttttttatcg actttcccga ccgccgtcag cggaagtgaa
     9121 tccacacact ccacgcgatc cggtaattta aattcggcaa taccctgttc acgcaggaaa
     9181 cgacgcacct gcaccgcgcg aagcggctct tttaccacca gataagcaca gcttttttcg
     9241 cccatcagct catcttccat actgaccagt gcggcgtaga tcaccgccgg atggcgcagt
     9301 aacaggtttt cgatctcttc ggcagcgatc ttctcgccgc cacggttgat ctgatctttc
     9361 tcgcgcccct gcacggtgat gtaaccctct ggatcaatag agatcagatc gccggaacag
     9421 taaaaaccgt tggcatcaaa ggcgctggca ttgtgctgcg ggcttttgta atagccacgg
     9481 aaggtatacg gcccgcgcgt catcagtcgt ccgacttccc cttgcggcag tggatttcct
     9541 tcggcatcgg caacccatac ttcgtcatcc ggacacattg ggtaaccctg ggtatggata
     9601 attttctccg cgctatcatc aagacgggta tagttcacca gcccttccgc cataccaaac
     9661 acctgctgca actggcaacc aatctcagcg ggaatacgcg ccgcaagcgt ggcagaaaga
     9721 cgtgcgccac cgacctgtaa cagtttcagc gaggcaagct gcgcccggct ttcgccttcg
     9781 gtcagcgcct gcaaccacag gctgactgcc ggcggcacca gcgcggtaac gttaatctga
     9841 tgtttttcaa tcaatgggaa gcaaagcgtg gcgctgggat cggccgccag aacaaccgtt
     9901 cctccggcaa gaaagacgcc cagcgatccc ggcgaactca tggcgtagtt atgagccgcc
     9961 gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac aaatctcgac gctacgacgc
    10021 acgctgtagt agtagtcgtt atgagtgcgc gggatcagct tcggtgtacc ggtggtgccg
    10081 ccggaaagct ggaaatagac cacttcatca gcaggtgacg gcgtagcagt aaaaccgtct
    10141 gccggatggt taatcgcatc ctgcaagtta tgctcaccgc tgtcgttgag tagctgcacc
    10201 acgcgaatgg aagaatgctc tgcaacaaat gtgttgagga aatcatcccc gctaaacagc
    10261 gcatgttggc gatcggcaat cagcaatgcg ggttcaatct ggctggcata ggcgttcagt
    10321 tcactacgct gatggctgaa caacgccagc accggcgcaa cgcccagttt cagcagcgcg
    10381 aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta ccagcgcggt ttcaccaggt
    10441 ttaatgccct gacggcgtaa actacacgcg aggttatccg ccgcctgatt cagctcccgg
    10501 taactcaact gtcgctcgcc gtcgataacc gcgatgctgt cactcgcagc gtggcgagtc
    10561 agaatgtcag tcagtggcaa atcctgccag tagccttttt cccgatagcg acgggcaaac
    10621 tcttccggcc agcgggtgaa tggaatgctc atcctcgctc cttaatgcaa tccaaaaacg
    10681 ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact cacccaacgg tgacgacgca
    10741 ggcacaatcc ccgctccggc aaacagacgc acctgatttt cccgcagctt cgcgcagcgg
    10801 atggtcacca cccattcgcc gttaccttcg ctgtcacacc aacccacaat gccgccaaac
    10861 agttcgcggt cgaacggctc cagttcagca ataacctggg tcgcggcctg atgcgggaaa
    10921 ccgctcagcg cgggggtcgg atgcagcaga caggccagag tcagtgcgtt ttcttgcgaa
    10981 ttcgctttac cttcaaaggg agttgcgaga tgccacagcg tcggcgtggt aatcaattgt
    11041 ggagaggaag gaacgtgtaa ctcactactg cgttcgcgca gtacctcttt catcgcctga
    11101 gtcaccagtt catgttcatg gcgatctttt tctgacgcca gcagacgatt acccgcttcg
    11161 cgatcgagca cttcatccgg ctgacgacgt gcggaaccgg ctaacggaat ggagctaaaa
    11221 cgctcaccgt ctttacgtag cagcagttcc gggctggccc ccagcagaac gccaccatct
    11281 gccagcggga catggaagtt gtaactaacc gggttttgcg caatcaaccg ttccagcaat
    11341 acgccactat caatggcggc gtcagtggtg atatcaatca accgtgacaa caccactttg
    11401 tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa ccatctgttc aaacgtggtt
    11461 tgctccggaa ttgcctggcg ttccaccacg ttcagcgact ggctgcgggt gaaacggcgt
    11521 gcggaagctt gtttttcctg acgggagaac gactgccagg attcaggaat atacagcgac
    11581 gaaggctgac gtggatcgaa aggaatcgcc ccgaccatca ccggattttt gatgccctgc
    11641 gctttggcat cggcaaacag cgcggcgagt ttttgctgga agggactgtc gggcgaatcc
    11701 ccgttcacag ccggttcatc gaagcgggcg aaacatcctg acgtcgtaaa actgcggtac
    11761 ggcgacataa agaaaaagcg attgggcgca agtgttgcca tggtctgctg tacttcctca
    11821 gccagtgacg tatccatatc atcctccaca aaatgataaa ggctttaata atgattatca
    11881 tttatatttt cggtcgctaa cctaaaagca aacgcgcact atgtcaactc ttgaggtaac
    11941 gcaatctgca actcggggtt gcatctggtt gtggttataa tgaaaatgag aagcattaac
    12001 ttcattaata acaggaggct gatttgtgag actcgccccg ctctaccgca atgcccttct
    12061 attaacagga cttttgtttt caggaatagc cgcagttcag gccgccgact ggccgcgtca
    12121 gattactgac agccgtggca ctcataccct ggaaagccag ccgcagcgta ttgtttccac
    12181 cagcgtcacc ctgaccggct cactgctggc gattgatgct ccggtgatcg ccagcggcgc
    12241 gaccacgccg aataaccgcg tcgcggatga ccagggcttt ttacgccagt ggagcaaggt
    12301 ggcgaaagaa cgcaaactgc aacggctcta tatcggcgaa ccgagcaccg aagccgttgc
    12361 cgcgcaaatg ccggatctga ttttaattag cgcaaccggc ggggattcgg cgctggcgct
    12421 gtatgatcag ctttccacca tcgccccgac attaatcatc aattacgacg acaaaagctg
    12481 gcaggcgctg ttaacgcaac ttggcgaaat taccgggcat gagaaacaag cggcagagcg
    12541 gattgcgcag tttgataagc aactggcggc ggcgaaagag caaatcaaat taccgccgca
    12601 gccggtcact gccattgtct atactgccgc tgcacacagt gccaatctct ggacgccgga
    12661 atcagcacaa gggcagatgc tggaacaact cggctttacg ctggcgaagt tacccgcagg
    12721 gttaaatgcc agccaaagcc agggtaaacg ccatgacatc attcagcttg gtggggaaaa
    12781 tctggctgca gggttaaatg gcgagtcact attcctgttc gccggtgatc agaaagatgc
    12841 cgatgctatt tatgctaatc cgctgctcgc gcacctgcct gcagtacaaa acaagcaggt
    12901 ttatgcgctg ggaaccgaga cgttccgtct ggattactac agcgccatgc aagtgctgga
    12961 acgacttaag gcgctgtttt aagcattaac cgtcagacgc tgtcacctgc ggcggcgtct
    13021 ggcgaaaacg tcgcaactcc accagcacca gcaataacaa cacgccgata atcaataaac
    13081 caaaaccgct cgcgcttgcg gaagcaaccg gtgtcatcat cgctcccaaa ccacccagca
    13141 gcgccgcgcc tatggcatcg cccgtcacgt tttgtgccgt ccacaaaccg ttaatccgcc
    13201 ctaacatcgc ttccggggtt tgcgtttgca gcattgtgta ttgcagcaac gagctgaccg
    13261 cactcaacca gccgaacagc gccagacaaa ccacgcctaa aatccacatc ggcatcaggc
    13321 caaacagacc aatggcgagg aacgatccca gcgtggagag cagcatcaat aaccctggtc
    13381 gcgcgctatg cgccagcttc ccgctggtta acgcgccaat agccgcgccg agcgggatcg
    13441 ccgcatagag aaaaccaatc tgtgccgccg acatctgcca gttgtcagcc agcgccggat
    13501 acagtacccg caccgcactc gccatcgtca ataaaccacc cagcagcgca atccctccca
    13561 ccagcgggct ggcgagtaga aaacgaaatc ctgccagtaa tgatttcaac ggatgctcac
    13621 gcggctgcgg tggcggtggc agtgccggaa ggcttaacaa cggtagcaag gtaataaacg
    13681 tgcccgccgc cgccagcccg tagttccagg ccacaccgcc agtcgccagc agtaaaccgc
    13741 caatcatggg cgaaatcacc gaccccagac gcacggtcaa catggtgatc gccccggcct
    13801 gcattaagtt ttcacgccct accagtgctg gtgtcgccgc caatagcgcc gtaacgccaa
    13861 gcgatgcgaa aaaaccatcc cataaaccaa gtaaatagat tgccagcaat gacggctccg
    13921 gcagcagcgc gttcaggcac agtccaatga agccaatgcc acaggtgccg cgcgccagca
    13981 aaatcacttt tttgcgctca tagcgatccg ccagcacacc gccgaccatc aggccaacaa
    14041 acatcgcgcc gccggtcagc gtcaccgaaa gccccacctg ccaggtggaa tgtgtcatca
    14101 tctggatctg caccggcacc gcgacgccga gcaaacccag agacacaatt gagatgaaac
    14161 gagcgaggaa tactgcgcga aacgccgggt gcgttttcaa caggctgagg ttaagcagcc
    14221 aggattgttt attcattaca atgccttgcc atcaaatatt ttaaatacct attcttaggc
    14281 tgcacatgct aacatatcca aataagatcg ataacgataa ttaatttcat tatcatggaa
    14341 gttcgtatgt ctggttctgt tgccgtgaca cgcgccattg ccgtgcccgg attgctgtta
    14401 ttactgatta tcgcgacggc attaagcctg ctcattgggg caaaatcact ccccgcttcc
    14461 gtagtgctgg aggccttctc cggcacctgc cagagcgccg actgcaccat cgtgctcgac
    14521 gcgcggctgc cgcgtaccct tgccggttta ctggcaggcg gcgcgcttgg ccttgccggg
    14581 gcgttaatgc aaaccctcac ccgaaaccca cttgccgacc ccggcttgct tggcgtgaac
    14641 gccggagcca gctttgccat tgtgctgggt gcggcgctgt ttggttactc ttccgcacag
    14701 gaacaactgg cgatggcctt cgccggggcg ctggtggcct cgttgattgt tgcctttacc
    14761 ggcagccagg gcggtgggca gttaagtccg gtgcgtttaa ccctggcggg cgtggcgctg
    14821 gcggcggtgc tggaagggct gaccagcggc atcgccctgc ttaatcctga cgtctacgat
    14881 caactgcgtt tctggcaagc cggttcgctg gatattcgca atctacatac cttaaaagtg
    14941 gtgctgatcc cagtgctgat cgccggagca actgcgctat tactgagtcg cgcgctgaac
    15001 agtttaagcc ttggcagcga caccgcgacg gcgctgggca gtcgcgtggc gcgcacacag
    15061 ttgattggtc tgctggcgat taccgtgctt tgtggtagtg cgacggcggt agttggccct
    15121 attgccttta ttggcctgat gatgccgcat atggcgcgtt ggctggtggg tgccgatcat
    15181 cgctggtcgc tgcccgtcac gctacttgct acccctgccc tgctgctgtt tgccgatatc
    15241 atcgggcggg tgattgtgcc cggtgaactg cgcgtttctg tggtcagtgc gtttattggt
    15301 gcaccggtgc tgatcttcct cgtgcgacgt aaaacgcgag gtggtgcatg atttacgtct
    15361 ctcgccgatt actcatcacc tgtttgctgc tgatttccgc ctgtgtggtt gcaggtatct
    15421 ggggattacg cagcggtgcc gtcacgctgg aaatctcgca ggtattcgcc gcgctgatgg
    15481 gcgatgcgcc gcgcagtatg acgatggtgg tcaccgaatg gcgtttacca cgcgtgctga
    15541 tggcgctgtt gattggcgcg gcactgggcg tcagcggtgc gatttttcag tcgctgatgc
    15601 gtaacccact cggcagccct gacgtaatgg gctttaacac cggggcgtgg agcggcgtgt
    15661 tggtggcgat ggtgctgttt ggtcaggatc tgacggctat cgcgctggca gcaatggtgg
    15721 gcggcattat cacttcgctg ttggtctggc tgctcgcctg gcgtaacggt atcgacacct
    15781 ttcggttgat tattatcggt atcggcgttc gcgccatgct ggtggccttt aatacctggc
    15841 tgttgctgaa agcgtcttta gaaacggcgc taacagcagg tttgtggaat gccggatcgc
    15901 tcaacggcct gacgtgggca aaaacctcgc cttccgcacc catcattata ttgatgctaa
    15961 ttgccgccgc cttactggta cgccggatgc gcttgctgga aatgggcgat gataccgcgt
    16021 gtgcgctggg cgtcagcgtc gaacgttcgc gtctgttaat gatgctggtt gcagtggtgc
    16081 ttaccgctgc ggcaacagcg cttgccgggc cgatttcctt tattgcttta gtcgcgccgc
    16141 acattgcccg acgcattagc ggcaccgctc gctgggggct aacccaggcg gcgctatgcg
    16201 gggcgctgtt actgctggcg gccgatctct gcgcccaaca actgtttatg ccgtatcaac
    16261 ttccggttgg cgtcgttacc gtcagcctcg gcggtattta ccttatcgtt ttgttaattc
    16321 aggagtctcg caaaaaatga ccgaatcagt agcccgtttg cgcggcgaac agttaaccct
    16381 gggatatggc aaatataccg ttgcggaaaa tctgactgta gaaatacctg atggtcactt
    16441 cacggcaatt atcgggccaa atggctgcgg taaatccacg ttgctgcgta ccttaagccg
    16501 cctgatgacg cctgctcatg ggcatgtctg gctggatggc gagcacattc aacattacgc
    16561 cagtaaagaa gttgcccggc ggattggtct gttggcgcaa aatgccacca cgctgggaga
    16621 tatcaccgtg caggagctgg tggcgcgtgg gcgttatcca catcaaccgc tgtttacccg
    16681 ttggcgcaaa gaagatgaag aggcggtaac gaaagcgatg caggccacgg gaataactca
    16741 tcttgcagat caaagcgtgg ataccctttc cggtgggcaa cgccagcgag cgtggatcgc
    16801 gatggtgctg gcccaggaaa cggcaattat gctgctcgac gaacccacga cctggctgga
    16861 tatcagtcat cagattgatt tgctggaact gttaagcgaa ctgaaccgcg agaaaggcta
    16921 taccctggcg gcggtgctgc acgatcttaa tcaggcctgt cgttacgcca gccatttgat
    16981 tgcattgcga gaagggaaaa ttgttgctca gggagcgccg aaggagattg tcactgctga
    17041 actgattgag cgcatttatg gtctccgctg catgatcatt gacgatccgg tagccggaac
    17101 gccgctcgtg gtgccgctcg gacgaacggc accttcaacc gcaaatagtt aaactaagtg
    17161 gtctgccatc atggcatcct gttttctgga tgccatcgca tagcgcaata acacgctacc
    17221 acaagccacc atcccgccga tcagcgcgga aaggatcaca ataatcgcct tacccggacc
    17281 gtcttttttc actggcaatg acgggcttaa ctgatattta aacggcgtaa aattcacatc
    17341 gttgatattt gcttttgtta actgctcaac aagatactga cgattacgta attcaccgtt
    17401 cagttccgca acgtcagtga ccgctttttc aatttccagt ttgcgttcaa taccgtctgc
    17461 gccgagagaa atagagaaat cggggtcatc tttaacggcc tgaccattac tgtaaacggg
    17521 ctttttaatt cctgccgcgt tggcaatgtc gagtgaataa ttgaggcgct gaatgtttgc
    17581 atcaagttga tttttcattt taatgcggtc ctgagccagt ttttcttttt caaactgggt
    17641 tttgatctcc agtttattac ggacgttttc tatcgactct ttcaccacca acgcagagat
    17701 ataatcgata tacccgctca aaacggtctg cgcctcttca ctggttggcg cggtaaaact
    17761 tagcgtccag gaggtataca gtgacggttc atcttttttc ttactggcat tgtcatcaac
    17821 cgctttcatt ttttcgctca atgcgacaat tgcgcgatgc aaatccagtt cgtcgatttt
    17881 cgcctctttt aattggtcca tcacataagg tgatgaacgc aggtactctt ccagcaagct
    17941 aaccgactga aacttcttga taaacaggtt aaatgcttct gtacgatcaa ttttgatatc
    18001 cagatccagc acacgaagct tagtgaatgt tttctccaac tcttgccact gaacaggttc
    18061 tggaggcgtg acaaccgccg cgctggtcca tttttgcggc aggatgaaag agatcagcaa
    18121 gcctgcacag gcaaacgcaa aaacgaccgc catgaccgtt tttttggccc gccataaaac
    18181 tgagattaga ttaagtaaat caatttcatt attactgggc gacgccagag gataatcggg
    18241 aaaatgagcg tcacttccct gtttaatatt cagtgatgac atggggtata acctgaaaat
    18301 gagtccaata gggggaaatc tccacggcgc tcactttacc agttggtgag aattatccga
    18361 agctgaagtt tgtaaattca ttccactgaa tgcaacattc actctattta agaaaaataa
    18421 ccagcaaact tataaattaa ataaatgata aacccataat tatttcctgc aattatgggt
    18481 ttatagataa tgttaattta tctgtttagc gttgcgcgaa taatcggccc aattttttca
    18541 aatgcccctg gagagataat atccacatgc gcacaatcct gacgatagat atccagctcc
    18601 gcaatccacg gcgaccaggc gcgttcgggg ctcatacctt cctgaagcgt acgttcagca
    18661 acaaacagcg tcgcttttcc gtcaaacggt acgctatgag cagtcgtcag caggcgcaca
    18721 gcatcagcgt agttgccttc aatggtggta aacaactccg ttgaagtact tccctgctgt
    18781 gccgccagga aggcctcgcg ctcgcggtta atctccgcca gcacttccgg gtccagacca
    18841 ttagcttctt tttcctgcca gttttgcgtt tctggcggcc aggtatccag caagccaaga
    18901 aatgccacct gttcgccacg ggcacgtagt cgcgccgcaa tgccctgcgc cagtgtaccg
    18961 ccaagggaat accccagcag gtaataaggg ccgtgcggtt gttgttcaag taacgttgcc
    19021 agatgcgctt cgcagacttc atccaggttc gtcgctgtct gcatggggcc atgagggcgc
    19081 ggcgactgaa tgccgataat cgaccatagt ggatcgagat aacgcgagag cacgctaaac
    19141 tgccaggcaa aaccggatgc cggatggaaa caaaacagcg tcgggccatt accttcacgc
    19201 aacggcagaa tggtttcgaa tcccatgcgc cgggagctgt cctcttcacc atcaataatc
    19261 gttgccagtt tggcgacggt tgacgcgacc atcacctgcc ccggcgtcac ctgacgggca
    19321 aactgccgac ttaactgcgc tgccagtttc attgccagta gcgaatgacc gccaagcgcg
    19381 aagaaatcag catcggcatc ctgcacgtca caacccagca acgacgcgaa tgccgcagcg
    19441 ataatcgttt cactgcccgc tttcggcgca cgccccggcg tttgtgcctt aagttcaggc
    19501 aacggtaagg ctttgcgatc cagcttgccg ttggcgctaa gaggtaactg tggaagttgc
    19561 agcagaacga ccggcgccat atgcggcggc aatgtttcgc gaagctgtgc ctgtaatgcg
    19621 ctggtatcca acggcagacc tgattgcgac accagatagc ccaccaactg acgcgcatca
    19681 ccaccggtgg ctgccgcctg gttaatcaca caggcgtggg taacggcttg ttcgacatcc
    19741 ggcagcgcct gcatcacgcg atcgatttcg cccagttcga tacgctgccc gcgaattttt
    19801 agctgatcgt cactgcgccc gaggtactcc accgcgccgt tatccagcca gcgggcaacg
    19861 tctccggtac ggtacatccg ttcaccaggg acaaaaggat cggcaatgaa gcggctggcg
    19921 gtcagatcgg gtcgtccaag atacccctgc gccagttgaa taccggtgag atagagatct
    19981 cccgccacac ccggcggcac cggatgcatc atcgcatcga gaatgcgcaa gcccgtattc
    20041 cacaccggat aaccaatcgg cacactgctg ccgcgcacct gtgccagttc ctcgccaaaa
    20101 gccggatacc aactcacatc taccgccgct tccgtcgggc catatagatt atgcaacggc
    20161 gcgcccgtta actgttgcca ttcgcggcat aaatcagccg gtaaggcctc accactacag
    20221 aaaacctgtt tcaacgtcgc gcaattctgg cgagcggttt gcggcgtcag cgatgcaaca
    20281 aatgccgcca gcatcgacgg cacaaagtgc gtggtcgtta cgccatattc ggcaaagaat
    20341 tgctgcatag cgagcgggtc gcggtgcgct tccggttcag ccatcaccag tttagccccg
    20401 gcaataaacg gccagaaaaa ctcccacacc gagacatcaa aactgcacgg cgttttttgg
    20461 gcaacgacat cttcacctgt aagtggataa tgattttgca tccacaacag gcggttaacg
    20521 atagccgtct gcccgaccat tacccctttc ggcctgccgg tggagccaga ggtaaagatg
    20581 atataagccg tgtgatgcgg ttgtgaaagt tgcagcggcg cactgccctg cggtgtaagc
    20641 ggggcgttat agcaaaggtt ggttaaatct ggaacatcgg caaagcgcgg cagttgatcg
    20701 tcggtggtga ttaacagcga cggacgcgca tcttccagca tcattttcag gcgatcgtcc
    20761 ggataaccgg tatccagcgg taaccaggcc gcacctgctt caacaatcgc atgtagcgcc
    20821 agggtcaaaa agaccgagcg cggtaatgcc accgccacgc tgtcccctgg tttaacgccg
    20881 tgctcacgca gcagattcgc cagcgccacc acctgctcgc gcatttcccg atagctgaac
    20941 tggtaacgcg catctgccag cgccggagca tccggtgttt ttgccgcttg ttctgccacc
    21001 agcgcgctaa gcgtggtttc tggaatctca acctgagtgg cgttgatctg cgccagctgc
    21061 gcatactcac ctggcagcat aatatcaaca tcgccgcaca acagagccgg atccgcagcg
    21121 aattgggcga tcagcatttt caggcgttca gcatgctgga ttaacgttgg ctcatcgtaa
    21181 tgctgtttat tggcgaggat ctcaatactc aaatcaccgt gttcatccgg gaacagggcc
    21241 agttcaaggt cattaaccgg accggttgcc agggtatggg tttgcgcctg aacaccagga
    21301 atatccagtt ggtaatcaaa taccttgata ttgagtaccg gaccaaacag cggttcatca
    21361 cctgccgctc gcccgctgtc acggacaatt tgttcggcat cgtaacgttg atgacgacgc
    21421 atttttttca gttgtgctgc cagtcgggtt gccagttccg gcagcgtttc ttgtgccgca
    21481 atgtgaatac ccaacggtaa aacgttgagc acgggtccgg tagccgtcag cgccgccgag
    21541 cccagtcgac gcataaagat aaatccggcg gcgtagtcca tgcgattgca caatcgcccc
    21601 agccaaaagg ctgccagcgc aagggctaaa tcggtacgct gcacacctga aagttgcgta
    21661 gccagctggc ggaattcccc gtcggtaaat tccagtttca ggcgtagaat atctgccgaa
    21721 gcgctgcgcc ccgctaaagg tgccggagaa agtgacgcgg gcggcggcag ttgacgacgc
    21781 tgttccgccc agaatgccgc atcacgctgc caggcttcgc tttcgcggta ttgctggtac
    21841 tcttccacta catcagcgaa aggcgtaaac ggcgaagcag gcgtttgttc gccacgcagc
    21901 aatgcgcagt aaatgttggc gatctgccga gtaatggccg ggaaactgaa gccatcgacc
    21961 agcaaatggt gataacgctg ataccagtac cagcggttat ctgccacctg tatcagctgg
    22021 tgaaagacca gtggtttacc gctatcgacg cgcaaatctt gttgcaaatc cgcctgcatt
    22081 aatgcctgcg cagtaccgtg cggatcaata ttggttcgca ggtcgataat ttctggcagt
    22141 tcgaatatca gcgcatcatc gacccattgc cagacttcgc cgttatcttc cgtaaaacgc
    22201 atccgcagcg tatctgcttg cgctaatccg gcaaccaccg cgcgggccag taatggcgca
    22261 tcaacctctc cggttaactc aacgtaatgc gccacgctcc aggcggaggg taattctgac
    22321 agtttttctg ccatccagat gccgggctgt gcggcgacca aaggtaaatg ctggctcatt
    22381 gtgcctcctg caactgggtg aaattcgccg gtgtcagagt acgccagtgg acttccagcc
    22441 actgctggca ggactcttgt gactgcggct gacacacaat gtcccagcct gccggtaagg
    22501 cgcattgttg cggccacaga ctgaattgcc cctgcgcatt gcgcaaaatg taaaacactc
    22561 cctgtggatc atcgaaggga ttactgaatg ccatattcaa ctcctgtcat ggaaaagtgg
    22621 ttgccagagg tcgattaacc cctgcatcaa gccaccgcgc caacaaagcg catcatgtcc
    22681 gccgtcaacc tgacgccaga aaatggattc ttttataggg tgtaattgcg catacagcgc
    22741 ctgattggcc cgcatgatca tcggctcgcg aatacccgct tccagcacaa tgcgcagacc
    22801 ttcggcgcta acttcaccag cttttagctt ttcaagtaac acgccctctt gctgcccgcc
    22861 ccgatgcggc caccagtacg atcctgactg gcttaataca cagccaaagc gttcaggcca
    22921 gtgcagtccg gcatacagcg cggacagccc accaaaactc tgcccggcga ccacggtgcg
    22981 atcggcgcga tcgctaaaag gggcaatagc tttcaccagg ggtaataact cttgctgtac
    23041 tgccagccag aaatccgcat tacacggcag ttcgtgggcg cggtgcgtgg tgtcgatagc
    23101 gtcgatcaac acatacacgg cgggaggaag ttgctgacga tgggtcagcg aaatcagcac
    23161 aggccagacg ggcatacttt gcgcccaaaa ttcgccatcg agcaaaactg ccagcgggcg
    23221 ttcttctgct gttacatcgc cggtggtaaa aatccataca cgacgtgaat ttttcaaccg
    23281 ttcacttttc cagataattt ctttggctgg tatttctggc gcttgcggac aatcccatcc
    23341 cggttgcaga ggcgcttgcg gcatttcgag tgcagaaaca gcgtgccctc gcccgccttt
    23401 ccagctttgt ggattcagcg gatcggctat tgcctggggt aatagttttc gccagccttc
    23461 acgtaattcg aggcgatcgg gggatggtgc agaaaaaatg tcatcgcgtt cggtgggaat
    23521 aaagcagtag ctgccgcgcc agttggcatt gagttgtgtc gtccactgcc agacatcagt
    23581 acctgcaatt cgctgcatcg actggggctg gctgttctga tgatgatcgg tcacaccagt
    23641 gatgtagatc catacgcgct ttatcgtcga gtattcttca gaaccttggg gatcacgcca
    23701 ccagaaagtg acctcaaaca tttcgtcatt cagacgctgc cattccgggc catgtttcga
    23761 ctgccaccag ctctcacttc ctacttttaa cgccgtcaca ccataacccc atgtttactg
    23821 tgcaattttt cattgattgc agaaatatat tgataatatt attgataact atttgcattt
    23881 gcaatagcgt aatggcgcgc cgtgggaagc gcggacatta attaaccaac tgcactgcgt
    23941 gtctttcagg atcaaaggtt ttcgcggtag cgggatgcgt cgtgttgatg acgaccatgc
    24001 ccgacagttg caattcgtgg caaaaatgca ggaataaaac aatgaacaag aagattcatt
    24061 ccctggcctt gttggtcaat ctggggattt atggggtagc gcaggcacaa gagccgaccg
    24121 atactcctgt ttcacatgac gatactattg tcgttaccgc cgccgagcag aacttacagg
    24181 cgcctggcgt ttcgaccatc accgcagatg aaatccgcaa aaaccccgtt gcccgcgatg
    24241 tttcggagat catccgtacc atgccaggcg ttaacctgac cggtaactcc accagtggtc
    24301 agcgagggaa taaccgacag attgatattc gcggtatggg tccggaaaac acgctgattt
    24361 tgattgacgg caagccggta agcagccgta actcggtgcg tcagggctgg cgtggcgagc
    24421 gcgatacccg tggtgatacc tcctgggtgc cacctgaaat gattgaacgt attgaagttc
    24481 tgcgtggtcc ggcagctgcg cgttatggca acggcgcggc gggcggcgtg gttaacatca
    24541 ttaccaaaaa aggcagcggc gagtggcacg gctcctggga cgcatatttc aatgcgccag
    24601 aacataaaga ggaaggtgcc accaaacgca ctaactttag cctgaccggt ccgctgggcg
    24661 acgaattcag cttccgcttg tatggcaacc tcgacaaaac ccaggctgac gcgtgggata
    24721 tcaaccaggg tcatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta ccagccgggc
    24781 gcgaaggggt gatcaataaa gatattaatg gcgtggtgcg ctgggacttc gcaccattgc
    24841 aatcgctgga actggaagcg ggttacagcc gccagggtaa cctgtatgcg ggcgataccc
    24901 agaacaccaa ctctgacgct tacactcgct cgaaatatgg cgatgaaacc aaccgcctgt
    24961 atcgccagaa ctactcgctg acctggaacg gtggctggga taacggcgtg accaccagca
    25021 actgggtgca gtacgaacac acccgtaact cgcgtattcc ggaaggtctg gcgggcggta
    25081 ccgaagggaa atttaacgaa aaagcgacac aggatttcgt cgataacgat cttgatgacg
    25141 tgatgctgca tagcgaagtt aacctgccga ttgatttcct cgttaaccag acgctgacgc
    25201 tgggtacgga gtggaatcag cagcggatga aggacttaag ttccaacacc caggcactga
    25261 ccggaacgaa taccggtggc gctattgatg gcgtgagtgc caccgaccgt agcccgtatt
    25321 caaaagcaga aattttctcg ctgtttgccg aaaacaacat ggagctgact gacagcacca
    25381 tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt cggcaataac tggagcccgg
    25441 cgctgaacat atcgcaaggt ttaggcgatg acttcacgct gaaaatgggc attgcccgcg
    25501 cctataaagc gccgagcctg taccagacta acccgaacta cattctctac agtaaaggtc
    25561 agggctgcta tgccagcgcg ggcggctgct atctgcaagg taacgatgac ctgaaagcag
    25621 aaaccagcat caacaaagag attggtctgg agttcaaacg cgacggctgg ctggcgggca
    25681 tcacctggtt ccgtaacgat tatcgcaata agattgaagc aggctatgtt gctgtagggc
    25741 aaaacgcagt cggcaccgat ctctatcagt gggataacgt gccgaaagcg gtggttgaag
    25801 gtctggaagg atcgttaaac gtaccggtta gcgaaacggt gatgtggact aataacatca
    25861 cttatatgct gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc atcccggagt
    25921 atacgttgaa ctcaacgctg agctggcagg cacgagaaga tttgtcgatg caaacgacct
    25981 tcacctggta cggtaagcag cagccgaaga agtacaacta taaaggtcag ccagcggttg
    26041 gaccggaaac caaagaaatt agtccttaca gcattgttgg gctgagcgcg acgtgggatg
    26101 tgacgaagaa tgtcagtctg accggcggcg tggacaatct gttcgacaaa cgtttgtggc
    26161 gtgcgggtaa tgcccagacc acgggcgatt tggcaggggc caactatatc gccggtgccg
    26221 gggcgtatac ctataacgag ccgggacgta cgtggtatat gagcgtaaac actcacttct
    26281 gatgctaacg tcagattgtt gacaaagtgc acgtcgttca tgccggatgc ggcgtgaacg
    26341 ccttatccgg cctacaaaaa tcttgccaat tcaatatatt gcaggaccat gtaggcctga
    26401 taagcgtagc gcatcaggca gttttgcgtt tgtcatcagt ctcgaatatg gtcgatatga
    26461 aaactacgca tacctccctc ccctttgccg gacatacgct gcattttgtt gagttcgatc
    26521 cggcgaattt ttgtgagcag gatttactct ggctgccgca ctacgcacaa ctgcaacacg
    26581 ctggacgtaa acgtaaaaca gagcatttag ccggacggat cgctgctgtt tatgccttgc
    26641 gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct acgccaaccg gtgtggcctg
    26701 cggaggtata cggcagtatt agccactgcg gggctacggc attggccgtg gtttcccgtc
    26761 aaccgattgg cgttgatatc gaagaaattt tttctgcgca aacagcgacc gaattgacag
    26821 acaacattat tacaccagca gaacacgagc gactcgcaga ctgcggttta gccttttctc
    26881 tggcgctgac actggcattt tccgccaaag agagcgcatt taaggcaagt gagatccaaa
    26941 ctgatgcagg ttttctggac tatcagataa ttagctggaa taaacagcag gtcatcattc
    27001 atcgtgagaa tgagatgttt gctgtgcact ggcagataaa agaaaaaata gtcataactc
    27061 tgtgccaaca cgattaattg acaacatctg gtacgattcg cccgcagcca tcactgactg
    27121 cgggcgaaag tgtaaagcag gtgccttacc actctgacct gacaaccgga tatgcgggga
    27181 ttgctccccg cacaacggct acttcttcgg ttcgtaagcg agaacagcct taaactcaat
    27241 attacgttcc tttacggtga actcacacag cgagtctccg accagaagcg taaatcccag
    27301 caccgtcaaa cacagcacta tgactgccac aagggcatat ttcgtcagca tgtttatatt
    27361 gcctccatgc gaagaaggga ctaccatccg agttgttggg gctcggagtg gcagccccag
    27421 gattgataga aatatctcct ggggcttcat ctttccagac ctcagagtta acctgaaacc
    27481 ggaaagcttc aggcacccgc cgttatctta cccttcctta gcaaaatagc tgtttattta
    27541 tacagtgaat cctgtttcgg acttctgtcg gcaaaactca tcattgctct taaaacgaaa
    27601 tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag caggtgcctt accatcctga
    27661 cctgacaacc ggatatgcgg ggattactcc ccgcacaacg gctacttctt cggttcgtaa
    27721 gcgagaacag ccctgaactc aatattacgt tccttcaccg taaactcaca cagcgagtct
    27781 ccgaccagaa gcgtaaatcc cggtaccgtc aaacacagca ctatgactgc cacaagggca
    27841 tatttcgtca gcatgtttat attgcctcca tgcgaagaag gaactaccat ccaaattgtc
    27901 tggtttggag tggcagcccc agggttgata gaaatatctc ctggagcttc atctttccag
    27961 acctcagagt aaacctgaaa ccggaaagct tcaggcaccc gccgttatct tacccttcct
    28021 caacaaaata gctgtttatt tatacagtaa atcctgtttc ggacttctca ctcctcattt
    28081 aatcaactga agctgagccc gttttttctg catgcctcaa tcgtcaaaat tcttcgccac
    28141 cagccagccg tgccataact tgtcacattg caaaagtagc caaattgctt cagtcaccat
    28201 caccaaaaaa agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata
    28261 ctgaatagaa aactccaaca taagagaaac ctatgccatt acccgatttt catgtttctg
    28321 aaccttttac cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact
    28381 taagccagga ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg
    28441 taaagcacga tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca
    28501 accaggctgc cgggcaattt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc
    28561 atcatctgga aatttgcggc ggtggcacgc acccgtttca gaaatggcag cgtcaggagg
    28621 tatgcgacaa cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg
    28681 cgaccgtttt tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt
    28741 tgctgcacgg cttgtcacgg tttgtgccgc actttatcgc cctttccgcc gcgtcgccat
    28801 atatgcaggg aacggatacg cgttttgcct cctcacgacc gaatattttt tccgcctttc
    28861 ctgataatgg cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct
    28921 gtctgagtta caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca
    28981 gtcctcattt tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg
    29041 cggtaaatat ggcgggatta attcaggcca ccgcccactg gttactgaca gaacgcccgt
    29101 tcaaacataa ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgccgct
    29161 atgggctgga aggcgtcatt accgatccgt acactggcga tcgtcgacca ctaacggaag
    29221 acaccttgcg attgctggaa aaaatcgccc cttctgcaca taaaattggt gcatcgagcg
    29281 cgattgaggc cctgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg
    29341 atttcgtcgc cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg
    29401 ccggtgacta agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact
    29461 tacatttaac aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt
    29521 gctgatggct ttcctctctt gcctgctgct gcccgccccc gcactggggc ttacgctggc
    29581 acaaaaactg gtgaccacgt tccatctgat ggatcttagt cagctttaca ctttattgtt
    29641 ttgtctgtgg tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg
    29701 acgctggttc tcgctggcgg attaaacgtc gattatcgcc gtacttcgcg gcatactttg
    29761 cttattctcc ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga
    29821 aacgcctggc ccttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg
    29881 atgtttttaa aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct
    29941 ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca
    30001 ttaagccagg gtatcacatg aataaaaagc actggatttc ggtgtatccc ggcgaggaaa
    30061 tctccgaagc gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc
    30121 tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag cggaaatcta tagcgcattt
    30181 ttctcgctta ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttactttcac
    30241 atggagtctt tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg
    30301 atgccagcaa aaaacttacc ccggaacagg ccgagcagat caaaactctc ctgcaataca
    30361 gcccatccag caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta
    30421 aagcgcgtgt tgccaaatcc gctgccggta attatgtgtt caacgaacgt aaaatacttg
    30481 atgcctcgca cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc
    30541 tggttgttga ccaggaagat gctgatggcc gctttgccac gccggaagcg aaagccgcga
    30601 acgataaagg tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag
    30661 agtggatggc aaaacaggtt tatctcaacg tcggtaattt cctgctcggc gtggcggctc
    30721 tgggtctgga cgcggtaccc atcgaaggtt ttgacgccgc catcctcgat gcagaatttg
    30781 gtctgaaaga gaaaggctac accagtctgg tggtagttcc ggtgggtcat cacagcgttg
    30841 aagattttaa cgctacgctg ccgaaatctc gtctgccgca aaacattacc ttaaccgaag
    30901 tgtaattctc ccttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc
    30961 ataaccctgt ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga
    31021 tttaatatcc catgttgaag atttagcggg tattgagatc gatcacacca cctcgatggt
    31081 gatgattttc ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt
    31141 ggtactgcgg accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat
    31201 tacccagaat aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat
    31261 tcaggcggta ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc
    31321 gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc tcgctgctgg atgttatttt
    31381 gaatctggcg cagaaattcc cggcagcatc tcagttaccg ctgaaaggga tatttcaggg
    31441 gattaaactg atcggcgcga ttctggtcgg cattttgatg atctcgctgc tgattggtca
    31501 gtcgccagcg attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt
    31561 taaagatccg attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa
    31621 actgggcgac tggctggaga tgccgaaata cggcgcggat ggcgcggtga tcgatattgg
    31681 gttaaccacc gtcaaggtgc gtaactggga caataccatt accactattc ccacctggtc
    31741 tctggtttct gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat
    31801 taagcgcagt atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca
    31861 acgtctgaat aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa
    31921 tgagtggaat cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa
    31981 tattggaacc tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa
    32041 agatatgacc ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat
    32101 ctatgcgttt accaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt
    32161 cgatcacata tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg
    32221 caatgatatt cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc
    32281 gaatggatga gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc
    32341 agcgttttaa atgctacaaa caggaataca atccacaccg gcagcaggat cgctgacaag
    32401 cgcatatcat ccatcgtgca catcagcagc aaaatcatgg cgaggaaggc gatgcaaaga
    32461 tagttgccga acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc
    32521 gctgcacgaa aacgcagatg cgccagacag atcataatcc agttcaacag cagcgttgct
    32581 accaccagcg ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc
    32641 accagcgaag tgatcgctcc ggaaagcatc agcgagttaa tcggcacgcc gcgacggcta
    32701 acacgggtaa ggaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg
    32761 ttagagtaaa ccccgctgtt atacactgac agcgatgcta ccagaatgac gaagttcagc
    32821 gcagaagcta ccacgttgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg
    32881 gatttcactt ccacccacgg atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac
    32941 agcaggatgc gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc
    33001 gcttcagcgg cagtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc
    33061 agcgacaaaa tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg
    33121 tcgatactgg ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc
    33181 ataccgatga ttgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag
    33241 cgcacgttca ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac
    33301 gttggaacat ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt
    33361 cccaccagca cgaacatcac ccagtagttc cagccggaga ggaacccggc gaacggtccc
    33421 cagtatttat aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg
    33481 ccgagctggc gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca
    33541 gccggacccg ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg
    33601 ccacccagcg caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct
    33661 tgattcgacg cagtatcttc cgatacgttt gacgcgtttt tcacgccttt cccctgtgtg
    33721 tcttttttgt tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca
    33781 cacggttaaa ccgggtatcc tgcttttatt tccgtacccg atgtcggtgc agccacatca
    33841 gcttatacgc tgccgggata ataaacagcg acagcaaagg cgcggtgatc atgccgccaa
    33901 tcatcggcgc ggcaatccgg ctcatcacct ctgatcctgc ccccgttccc cacagaatcg
    33961 gcagcagacc agcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg
    34021 cgccgtgatg taacgcctca tccagcttct gctcgctgaa tgtttgcgga ttattcaacg
    34081 acggctcggc ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg
    34141 cgaccccggc aagggcaata aaaccggtgc ccgtcgccac agaaagatga aagcccatcc
    34201 accacagcaa ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg
    34261 cttcgcccac ccgacggaac gccagataca acagcacgaa gatgatcatc aacgtcatcg
    34321 gcaccatgag tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg
    34381 ccacgctggt gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa
    34441 ccaccgacac catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat
    34501 tctcggtttt cagcatcgac ggtccggtag agactttaac gtcggccacg tctgccaggg
    34561 tgatttgctg cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc ggactatcgc
    34621 gccagctttg cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc
    34681 caaccatcgc cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac
    34741 cgtaacgtgc ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc
    34801 gctcggcaag cgcagaagcc acgcctggca ccgttcgtgc cacctcttca atttgctccg
    34861 ccatcgcgtc gatatccgcc agcacagtgc cggaaacttt tatgccgata gggcttttaa
    34921 tgccggttga gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc
    34981 ccggcagacg cacggtgtta tccagttcct caatgatttt gtccattgtc atgcctggcc
    35041 gccactgctc ctgcggctta agctggatgg tcgtttctac catctccagc ggagcagaat
    35101 cggtggcggt ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca
    35161 taattagctt gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca
    35221 gcgtcgatgg catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa
    35281 ctttattgag cggccagaga accgtcagca ccgaaagcgc tgccaccaac agcgtggttt
    35341 tcggccagtg cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga
    35401 gcgggttact gctttccggc ggaattttgc cacggatcca gtaacccatc aggataggga
    35461 tcactacgat cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccagcg
    35521 ggccaaacag acgcccttcc tgcccttcca gggtgaagat cgggataaac gacagcgtga
    35581 taatcagcag actgataaac agcgccggcc ccacttcaac agatgcattg gtaatcacct
    35641 gccagcgcgt tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc
    35701 gtttatgtgc attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg
    35761 caatgccacc caacgacata atattggcat tcagcccctg gaagtgcatg acaataaaag
    35821 caatacacaa ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga
    35881 gaaacagcgc acagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt
    35941 tgtcgatggc ccggtcaatg agctggctgc gatcgtatgt tgtaactatc tccacgcctt
    36001 ccggcagact gcttttcagc gtttccagtt tgtccttcac ggcggcgatc acttcacggg
    36061 cgtttttgcc ggagcgtagg atcaccaccc cgcccacaac ttcgccgttc agttcggcaa
    36121 tgccccggcg catctccggg ccaacctgga tcttcgcaac atcacgcaga taaacaggca
    36181 cgccattttc actggctttt aaaacgatgt gattaaagtc gtcgagcgtt tgtagatagc
    36241 cgctggcgcg caccatatat tctgcttccg ccagttcgat tgacgaaccg cccgcttcct
    36301 ggtttgaagc atccagcgcg ctttttactt cggcgagact gatgccatac tgcgccaggc
    36361 gctgaggatc gataaccacc tgatactctt tcaccacgcc gcccaccgac gccacttccg
    36421 caacgtcagg gatggttttt agctcatatt tgagaaacca gtcctgcaat gagcgtaaat
    36481 cggccagatc gtgcttaccg ctgcgatcca ccagtgcata ttcatagatc cagccaacac
    36541 ccgtggcatc tggtcccagc tcggcgctga ctcccgcagg cagcttaccc tgtacctggt
    36601 tgaggtactc cagcacccgc gaacgcgccc agtacggatc ggtaccatct tcgaaaatga
    36661 catacacata agagtcgcca aactgcgaga aaccacgcac agtcttcgcg ccaggcaccg
    36721 acaacatggt ggtagttagc ggataagtca cctgattttc aacgatttgc ggtgcctgac
    36781 cgggatagct ggttttaata atcacctgca catcggagag atccggcaac gcatccactg
    36841 gcgtgttaat gatggtccag gtgccccaga tactcagaaa taacgcgccc atcagcacca
    36901 aaaaacggtt cgccaccgag cgacgaataa tccattcaat cattggttat tccctcaatg
    36961 cgcatgggta gcactttcag agcgcatccg ctccagtgcg ccagaaatat tggcttcgga
    37021 atcaatcagg aacaggccgc tggaaaccac cttttcacct tccgccagac cagagcgtaa
    37081 tgcggtgaca ccttgcgatg cctggaaaac ggcaacgcgt tttggtacaa agcgcccgtc
    37141 ggcatcaacg gtaatcaccc gctgttcgct gccggtatca atcagcgcct gtgacggaat
    37201 gagcagcatc ggttcgctgg cggtattgag ttgcaaccag gcgttcattc ccggttttag
    37261 cgcctcgtcg gcgttgtcga cttccagacg cagctgcagc gtgcgggtcg cggcatccac
    37321 gccaggtagt agcgtccatt tgcggatggt gagtgtttta tcaggtcgcg ccggaacggt
    37381 gagcgtaaac tgcgaggcat ctttcaccag ccaggcgatg gactccggga tcgcagcagt
    37441 gacccacacc gggtccatac cctgaatttt cgctaccacg ttatctttgg cgatattcat
    37501 tcccgcgcgc agatcaaacg cggtgatcac gccatcaatg ggtgctttga gcgtaaagcg
    37561 agtctggatt ttttgcgtgg cgatcaggcg gcgaatatcc gcctccggca ttcccgccag
    37621 tcgcagccgc tcaagaatgc cttcggtctg ggtcgccgta ccgccggttt cgcgcagcag
    37681 taaatactca ctctgcgctt ccacccagtc gggaatggtc aggtcgagaa gcggcgcgcc
    37741 cttttgtact ttatcgccca cggtaagcgg atacaccttg tcgataaacc cggcggcgcg
    37801 agcctgcaca atggcatact gatactcgtt gtaactgaca ttcgccggga aactctgggc
    37861 aaaagtcagc ggcccgcgcg tgacggtagc cgttttcacc cccagattct gcgtctgagt
    37921 cgggtcaatg cgcacaccag acgcagaact ctcttcatcg gcatatttcg gcaccagatc
    37981 catatccata aacggcgatt tacctggttt atcgaaccgc gtattgggat acatcgggtc
    38041 gtaccagaat aagattttac gttctgcggt cgacgttttt tctgcaggcg gttccgcctt
    38101 tgcaaaccag gtaaaacctg ccgcagaaat aataccgccc gcgatcatgc tgccgataat
    38161 aagcgcgatt tttttcatct cattaaacct gggttactgg ctgactttaa tatcctgtaa
    38221 taaagaaagg ttgccctgct ggacaaaatt aaacgccact ttgtcgccgg ttttaattcc
    38281 actcattttc gtctgcgggg tgatggtaaa gcgcatggtc atctccggcc agttcacggc
    38341 agcaatcgga tcgtgatgga tggtgatttt tttgctttcc agatcaaaac cttttaccac
    38401 gccggtggca ctaatgacct gtggttgtgc ttcgctcatg gtttcatgat gatgttcgtt
    38461 agcctgggca ttaaagccaa taacggtaaa cagactgaac attgcgactt gcagtgcttt
    38521 tttcatttat tttctcctgg agttaaaaat tattgctgcc aaccgccacc aagtgcggta
    38581 tataaagaaa tttcgttgac ctgacgggca taattcaggt caagtacggt ttgtcgggtt
    38641 gcaaataaag aacgctcggc atccagcact tccagataac ttactgcgcc gtgctgatat
    38701 aatgcccgcg cccgttgcaa agtaatttgc agcgatgcca gataacgctg ctgggcgcta
    38761 atttgatcgt tcaggctttg acgtaatgcc agcgcatctg ccacttcttt aaaggcgttc
    38821 tggattttct gttcataatt cactaccgac tgctgctggc gaatttcggc gatatccaga
    38881 ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttaggaat aaaattccac
    38941 atcccgctgc tggcgttaaa taatgacgac agatcgctgc tggcggttga tattccgctg
    39001 gtcaggctga tagacgggaa aaatgccgcg cgtgcagcac caatattggc attagccgcc
    39061 attaacgcat gttcagcttc cataatatcc gggcgctgca ataagatttg cgacgacaag
    39121 cccgccggta atttaacgct ttgcaggctg tcgctgttta ctgtctgcgc ttgcggcagc
    39181 ttgccgtagc ttcccagtaa cagttgcaat gcattattcg cctgcgccag ttccccctga
    39241 cgtttagcga tgtcgctgcg ggtactttca atcaccccgc gagcctgttc cagcgccaga
    39301 acattactgc tacctgtcaa cagttgtttt tcgacaaacg catatgactg ctgataatta
    39361 cgcagcgttt cttcggctat ttgcaattgc gcatacgcca gctgctgatt gaaatagctt
    39421 tgcgcgacat tagaaaccag cagaatatgc accgcgcgct gagcttcctc agtggctaaa
    39481 taattttgtc gctcggcttc gctcatgttc tttaagcgcc cgaaaaaatc gaggtcaaag
    39541 ctggcgttaa ggccagtaga aaactcccgc gtcgtggctg aatcgccttt aagattgccg
    39601 ctccagctgc cgctgccctc accattgagc tgtgggtagc ggtcggcatc agtcagacga
    39661 tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg gttattctcc
    39721 agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg ccagcccgcg
    39781 ttctgatagt tatctgctgc gttaaccagg ccgttctggc tgagtgagaa ctgctgcggt
    39841 acgggcattg ccgggcgctg ataatccggt gccagtgaac aaccggttag cgcaagggcc
    39901 acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctggt gattttatac
    39961 cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca aaattgtcat
    40021 tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg gcaacatgcg
    40081 gaggaaatat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa tacttgacca
    40141 aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg aatggctacc
    40201 atctggcgat gaccggagat tatgacctga taatcctcga tattatgctg ccggacgtga
    40261 acggctggga tatcgtgcgc atgttgcgct ccgccaataa agggatgccg attctgttgc
    40321 ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg gccgatgact
    40381 atctggtgaa gccgttcgcg tttgctgaac tgctggcgcg ggtgcgcact ctcctgcggc
    40441 gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatttgatg gtcgatctcg
    40501 tcagccgcaa agtcacccgc agcggcacgc gcatcactct gaccagtaaa gagtttactc
    40561 tgctggagtt ttttctccgc catcagggcg aagtgctgcc ccgctcgctt atcgcctcgc
    40621 aggtatggga catgaatttt gacagcgaca ctaacgccat tgatgtggcg gtgaagcggc
    40681 tacgcggcaa aatcgacaac gactttgagc cgaagctgat tcaaaccgtg cgcggcgtgg
    40741 gttacatgct tgaggtgccg gatggtcagt aagccatttc agcgcccgtt ttcgctggca
    40801 acccgcctga ccttttttat cagcctggcc accatcgcgg cgtttttcgc ctttgcatgg
    40861 atcatgatcc actcagtaaa agtgcatttt gccgagcagg atattaatga tttaaaagag
    40921 attagtgcca cccttgaacg cgtactaaat caccctgacg aaacgcaagc ccgacgctta
    40981 atgacgctgg aagatatcgt cagtggttat tccaatgtgt tgatttccct ggcagatagc
    41041 cacggtaaaa cggtgtatca ctcccccggt gcgcctgata tccgcgagtt tgcgcgcgat
    41101 gccatacccg ataaagacgc gcggggcggc gaggtgtttc tcctctccgg cccgacgatg
    41161 atgatgccag gccacggtca cgggcatatg gaacacagca actggcggat gatcagtttg
    41221 ccagttggcc cactggttga cggcaaaccg atttatacgc tctacatcgc gctttcgatc
    41281 gattttcatc ttcattacat aaatgatttg atgaataaac ttattatgac cgcatcggtc
    41341 atcagcatcc tgatcgtctt tatcgtgcta ttggcggttc ataaaggtca cgcgccgatc
    41401 cgcagcgtca gccgtcaaat ccagaatatc acctcgaaag atctcgacgt ccgccttgac
    41461 ccgcagaccg tgccgattga gctggaacag ctggtactgt cgttcaacca tatgatcgag
    41521 cgtattgagg atgtctttac ccgccagtcc aatttctcag cggatatcgc ccacgaaatt
    41581 cgcacaccaa ttactaatct cataacgcaa acggaaatcg ccctcagcca gtcgcgcagc
    41641 cagaaggagc tggaagatgt gctctactct aatctcgaag agctgacgcg aatggcgaaa
    41701 atggtcagcg atatgctgtt tctcgcccag gccgataaca accagttaat ccccgaaaag
    41761 aaaatgctca acctggcgga tgaagtcggc aaagtgttcg attttttcga ggctttagcg
    41821 gaagatcgcg gcgtggagct gcagtttgtt ggcgacgagt gccaggttgc gggcgatccg
    41881 ctgatgctgc gtcgggcgtt aagcaacctg ctttcgaacg ccctgcgtta tacgccaccc
    41941 ggagaggcta ttgtagtgcg ctgccagacg gtcgatcatc tggtgcaagt tatcgtcgaa
    42001 aaccccggta cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg tttctaccgc
    42061 gttgacccct cccgccaacg aaaaggcgaa ggtagcggca ttgggctggc gatagtgaaa
    42121 tcaattgttg tcgcgcataa aggcacggtt gcggttacgt caaatgcgcg ggggacaagg
    42181 tttgtgattg tgttgccgga gagggggtga ttattaagtt caaattcagg gcgagggttt
    42241 acggcgtttt tcgctaaatt tccatcatta taaacaagcc acctatcata taaagcaatg
    42301 aat
//
LOCUS       DAZKFZ010000016        41843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000016 DAZKFZ010000000
VERSION     DAZKFZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(39..115)
                     /locus_tag="ACHOQW_000822"
     tRNA            complement(39..115)
                     /locus_tag="ACHOQW_000822"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79..81),aa:Arg,seq:acg)
     gene            complement(119..211)
                     /locus_tag="ACHOQW_000823"
     tRNA            complement(119..211)
                     /locus_tag="ACHOQW_000823"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Ser,seq:gct)
     gene            complement(527..712)
                     /gene="csrA"
                     /locus_tag="ACHOQW_000824"
     CDS             complement(527..712)
                     /gene="csrA"
                     /locus_tag="ACHOQW_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP9571946.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(947..3577)
                     /gene="alaS"
                     /locus_tag="ACHOQW_000825"
     CDS             complement(947..3577)
                     /gene="alaS"
                     /locus_tag="ACHOQW_000825"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9571947.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3705..4205)
                     /gene="recX"
                     /locus_tag="ACHOQW_000826"
     CDS             complement(3705..4205)
                     /gene="recX"
                     /locus_tag="ACHOQW_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9571948.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4273..5334)
                     /gene="recA"
                     /locus_tag="ACHOQW_000827"
     CDS             complement(4273..5334)
                     /gene="recA"
                     /locus_tag="ACHOQW_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9571949.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5414..5911)
                     /gene="pncC"
                     /locus_tag="ACHOQW_000828"
     CDS             complement(5414..5911)
                     /gene="pncC"
                     /locus_tag="ACHOQW_000828"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP9571950.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6056..7141)
                     /gene="mltB"
                     /locus_tag="ACHOQW_000829"
     CDS             complement(6056..7141)
                     /gene="mltB"
                     /locus_tag="ACHOQW_000829"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP9571951.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7396..7958
                     /gene="srlA"
                     /locus_tag="ACHOQW_000830"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7396..7958
                     /gene="srlA"
                     /locus_tag="ACHOQW_000830"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            7955..8916
                     /gene="srlE"
                     /locus_tag="ACHOQW_000831"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             7955..8916
                     /gene="srlE"
                     /locus_tag="ACHOQW_000831"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            8927..9298
                     /gene="srlB"
                     /locus_tag="ACHOQW_000832"
                     /gene_synonym="gutB"
     CDS             8927..9298
                     /gene="srlB"
                     /locus_tag="ACHOQW_000832"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9571952.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9302..10081
                     /gene="srlD"
                     /locus_tag="ACHOQW_000833"
     CDS             9302..10081
                     /gene="srlD"
                     /locus_tag="ACHOQW_000833"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP9571953.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10187..10546
                     /gene="gutM"
                     /locus_tag="ACHOQW_000834"
     CDS             10187..10546
                     /gene="gutM"
                     /locus_tag="ACHOQW_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9571954.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SRGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10613..11386
                     /gene="srlR"
                     /locus_tag="ACHOQW_000835"
                     /gene_synonym="gutR"
     CDS             10613..11386
                     /gene="srlR"
                     /locus_tag="ACHOQW_000835"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP9571955.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            11379..12344
                     /gene="gutQ"
                     /locus_tag="ACHOQW_000836"
     CDS             11379..12344
                     /gene="gutQ"
                     /locus_tag="ACHOQW_000836"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP9571956.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12341..13855)
                     /gene="norR"
                     /locus_tag="ACHOQW_000837"
     CDS             complement(12341..13855)
                     /gene="norR"
                     /locus_tag="ACHOQW_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP9571957.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14042..15481
                     /gene="norV"
                     /locus_tag="ACHOQW_000838"
     CDS             14042..15481
                     /gene="norV"
                     /locus_tag="ACHOQW_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP9571958.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15478..16611
                     /gene="norW"
                     /locus_tag="ACHOQW_000839"
     CDS             15478..16611
                     /gene="norW"
                     /locus_tag="ACHOQW_000839"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP9571959.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(16739..18991)
                     /gene="hypF"
                     /locus_tag="ACHOQW_000840"
     CDS             complement(16739..18991)
                     /gene="hypF"
                     /locus_tag="ACHOQW_000840"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP9571960.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19144..19671)
                     /gene="hydN"
                     /locus_tag="ACHOQW_000841"
     CDS             complement(19144..19671)
                     /gene="hydN"
                     /locus_tag="ACHOQW_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP9571961.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19820..20830)
                     /gene="ascG"
                     /locus_tag="ACHOQW_000842"
     CDS             complement(19820..20830)
                     /gene="ascG"
                     /locus_tag="ACHOQW_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP9571962.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21090..22547
                     /gene="ascF"
                     /locus_tag="ACHOQW_000843"
     CDS             21090..22547
                     /gene="ascF"
                     /locus_tag="ACHOQW_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP9571963.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            22556..23980
                     /gene="ascB"
                     /locus_tag="ACHOQW_000844"
     CDS             22556..23980
                     /gene="ascB"
                     /locus_tag="ACHOQW_000844"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP9571964.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24104..24574)
                     /gene="hycI"
                     /locus_tag="ACHOQW_000845"
     CDS             complement(24104..24574)
                     /gene="hycI"
                     /locus_tag="ACHOQW_000845"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP9571965.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24567..24977)
                     /gene="hycH"
                     /locus_tag="ACHOQW_000846"
     CDS             complement(24567..24977)
                     /gene="hycH"
                     /locus_tag="ACHOQW_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP9571966.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(24974..25741)
                     /gene="hycG"
                     /locus_tag="ACHOQW_000847"
     CDS             complement(24974..25741)
                     /gene="hycG"
                     /locus_tag="ACHOQW_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP9571967.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(25741..26283)
                     /gene="hycF"
                     /locus_tag="ACHOQW_000848"
     CDS             complement(25741..26283)
                     /gene="hycF"
                     /locus_tag="ACHOQW_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP9571968.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26293..28002)
                     /gene="hycE"
                     /locus_tag="ACHOQW_000849"
     CDS             complement(26293..28002)
                     /gene="hycE"
                     /locus_tag="ACHOQW_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP9571969.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28020..28943)
                     /gene="hycD"
                     /locus_tag="ACHOQW_000850"
     CDS             complement(28020..28943)
                     /gene="hycD"
                     /locus_tag="ACHOQW_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP9571970.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(28946..30772)
                     /gene="hycC"
                     /locus_tag="ACHOQW_000851"
     CDS             complement(28946..30772)
                     /gene="hycC"
                     /locus_tag="ACHOQW_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP9571971.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(30769..31380)
                     /gene="hycB"
                     /locus_tag="ACHOQW_000852"
     CDS             complement(30769..31380)
                     /gene="hycB"
                     /locus_tag="ACHOQW_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP9571972.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31505..31966)
                     /gene="hycA"
                     /locus_tag="ACHOQW_000853"
     CDS             complement(31505..31966)
                     /gene="hycA"
                     /locus_tag="ACHOQW_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP9571973.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32178..32528
                     /gene="hypA"
                     /locus_tag="ACHOQW_000854"
     CDS             32178..32528
                     /gene="hypA"
                     /locus_tag="ACHOQW_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9571974.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32532..33404
                     /gene="hypB"
                     /locus_tag="ACHOQW_000855"
     CDS             32532..33404
                     /gene="hypB"
                     /locus_tag="ACHOQW_000855"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP9571975.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33395..33667
                     /gene="hypC"
                     /locus_tag="ACHOQW_000856"
     CDS             33395..33667
                     /gene="hypC"
                     /locus_tag="ACHOQW_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP9571976.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            33667..34788
                     /gene="hypD"
                     /locus_tag="ACHOQW_000857"
     CDS             33667..34788
                     /gene="hypD"
                     /locus_tag="ACHOQW_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP9571977.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            34785..35795
                     /gene="hypE"
                     /locus_tag="ACHOQW_000858"
     CDS             34785..35795
                     /gene="hypE"
                     /locus_tag="ACHOQW_000858"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP9571978.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            35869..37947
                     /gene="flhA"
                     /locus_tag="ACHOQW_000859"
     CDS             35869..37947
                     /gene="flhA"
                     /locus_tag="ACHOQW_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP9571979.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(37984..38337)
                     /gene="ygbA"
                     /locus_tag="ACHOQW_000860"
     CDS             complement(37984..38337)
                     /gene="ygbA"
                     /locus_tag="ACHOQW_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP9571980.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(38414..38548)
                     /locus_tag="ACHOQW_000861"
     CDS             complement(38414..38548)
                     /locus_tag="ACHOQW_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9571981.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            38624..41185
                     /gene="mutS"
                     /locus_tag="ACHOQW_000862"
     CDS             38624..41185
                     /gene="mutS"
                     /locus_tag="ACHOQW_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9571982.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTRLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            41291..>41843
                     /gene="pphB"
                     /locus_tag="ACHOQW_000863"
     CDS             41291..>41843
                     /gene="pphB"
                     /locus_tag="ACHOQW_000863"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9571983.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFI"
BASE COUNT         9842 a        11523 c        10978 g         9500 t
ORIGIN      
        1 tttttgatac cgctactaaa gccgtatcaa gtaagagatg gtgcatccgg gaggattcga
       61 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg
      121 gcggtgaggc ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg
      181 ggagcgcctt cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac
      241 ttgtttatcg ctcatcgtcg ctgcgtggcg cacatattac tttctgagac ttataagtca
      301 aacaattttt cccacacttt tatcgtttgc acacttcacg ctcaattagt ctgtaaaaac
      361 gtcaaaaaga gtgttttatc tacagaaaaa cggaggtctg gcagatagca gtaatgcaaa
      421 aaaatggaga cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag
      481 aaattttgag ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact
      541 gctgggattt ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct
      601 tcggggcatt tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga
      661 cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct
      721 ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc
      781 caaaaagtaa gcaatgacaa acacattaca tgtaagcagt catggcatta cattctgtta
      841 aacctaagtt tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta
      901 atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg
      961 cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac
     1021 cggcttgcgc catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac
     1081 caatcagttc ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag
     1141 aaaccttacc ttcggctacc gttgccagca cgataattgt cgaccccagc tgatttttta
     1201 aatcgtcaac catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca
     1261 gcttaacacc attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag
     1321 cttgttcttt aagctgttgt agttcttttt ctaactgacg cgtacgttcc agtactgagc
     1381 gcactttatc cgccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc
     1441 gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc ggttaccgct tcgatacgac
     1501 gaacgcctgc agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc
     1561 ggctggcgtg agtaccgcca cacagctcgg tggagaaatc gcccatgctc agcacgcgta
     1621 cacgctcatc atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga
     1681 gatccatgat gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt
     1741 cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca
     1801 ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt ccccagaacc tggcgcagcg
     1861 cagcgtgcat caggtgcgtt gcggagtgat tcaaacgaat acgggcgcga cgagcctcat
     1921 caacatcagc ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt
     1981 gaccaatcgc ctggccgtat ttctgcgtat cttccaccac gaaggagaag ttagcgcctt
     2041 tcagttcgcc tttatcacca acctgaccgc cggattccgc atagaatggc gtttgatcca
     2101 gcacgaccac agcttcctgg cctgcattga tggcatcaac cgctttacca tcaacaaaca
     2161 gcgcggtcac tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactgt
     2221 caacacggat cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcccgacgac
     2281 gctgctcttc cattgctgct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac
     2341 aaacatcagc cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagcagttt
     2401 caccatccag cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc
     2461 gctccagagt acgagcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct
     2521 gctggcgttt caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt
     2581 tgtagaagaa ggtttctttc gcgccgagca tattaccgtg acgcactgcg cgacgaatga
     2641 tacgacgcag tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga
     2701 acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggttg
     2761 cgccagtgac tttcgctacc gcctggatca acgtgcggaa caggtcgatg tcatagttag
     2821 agttaacgtg ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg
     2881 gcttcggcag cggttccatc gtgccatcgg cctggcggtt gaactgcatg aagacgatgt
     2941 tccagatctc aatgtagcgg tcgccgtctt cttccggact tcccggaggg ccaccccaaa
     3001 tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca
     3061 tctgccagaa gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac
     3121 gttcgcgcgg gatccctact tctttttccc agatttcgta ggcttcgtcg tcgctttcat
     3181 agacggtaac ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt
     3241 cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca
     3301 tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac
     3361 cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc
     3421 caaggaacac atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt
     3481 tatggggtac cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga
     3541 gaaacgcctg acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac
     3601 gaaatatcgc caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga
     3661 ataagataag ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt
     3721 cgccaaatat cctggatatc ttccatcaga tagccacgat agagcagaaa acgctggatc
     3781 ttaacttttt ctgaaaagac agttggcaaa ggttcaccat attttcgcgt cgcctgatcg
     3841 cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg
     3901 gaaatacctt tctgattcag ttcctggcga atacgcgcag gtccataacc tttgcggcta
     3961 cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac
     4021 caggcaataa cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg
     4081 cccataatcg gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc
     4141 agaatgcgta ccgcacgatc caacaggcga gcatatgccg ggcgacggga cgttgattct
     4201 gtcatggcat atccttacaa cttaaaaagc aaaagggccg cagatgcgac ccttgtgtat
     4261 caaacaagac gattaaaaat cttcgttagt ttctgctacg ccttcgctat catctacaga
     4321 gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct tctcgatctc
     4381 tttcgcggtt tccgggttat ctttcagcca ggcagttgca ttcgctttac cctgaccgat
     4441 cttctcacct ttgtagctgt accacgcgcc tgctttctcg atcagcttct cttttacgcc
     4501 caggtcaacc agttcgccgt agaagttgat accttcgccg tagaggatct ggaattcagc
     4561 ctgtttaaac ggcgcagcga ttttgttctt caccactttc acgcgggttt cgctacccac
     4621 cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa cagaggcgta
     4681 gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatca caccaatttt
     4741 catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt tacccgccag
     4801 cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt cgccgatttc
     4861 gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga taacgtctac
     4921 tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg tgtccggctg
     4981 ggagcacagc aggttgtcga tatcgacgcc cagtttacgt gcgtagattg ggtccagcgc
     5041 gtgttcagca tcgataaacg cacaggtttt accttcacgc tgcgctgcgg caatcacctg
     5101 caacgtcagc gtggttttac cggaagattc cggtccgtag atttcgacga tacggcccat
     5161 cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag agatggtttc
     5221 cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa attgtttctc
     5281 aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag ccatttttac
     5341 tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaagcaa ttatactgta
     5401 tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc aatgcatacg
     5461 cagtagcctg acgacgcacc gcatcacggt cgccgctgaa gcattcccgc agggtaatgc
     5521 cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc gacaggcttc tcttcactgc
     5581 cgccatccgg cccggcgata ccactaatag acacggcata atcagcacga gccgctttca
     5641 gtgcacctat cgccatttcc accacgacgg gttcactcac cgcgccatgc tgcgccagcg
     5701 tctcttcgcg tacgccgatc atttgcgctt tggcttcgtt actgtaggtg acaaatccgc
     5761 gttcaaacca ggcggaactg ccggcaatat cggtaatcac tttcgctacc caaccgccgg
     5821 tacaagactc agcagttgtt acggttgcgc cacgggcttt cagcgcctgc ccaacctgtt
     5881 cgcttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa aaatttatgc
     5941 cggacaagat agcactttcg tcaggaagat ggtgttggcg ttgcaatttt acgaggagga
     6001 ttcagaaaaa agctgattag ccagagggaa gctcacgccc ccctcttgca aatagttact
     6061 gcacgcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg taatgggtac
     6121 tgtggttata acgggtaatg gtgtagaagt tcggcagacc gtaccagtac tggtaacctg
     6181 ttccaacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc tgctgtggcg
     6241 ttaaacctgc cgcggcaagc tgcgaaatgc tgtacctggt tttgaagcca tttggcaagc
     6301 ctggagcctg accgtttgcc attaccgcga cctgatcgcc tttcacccag ccgtgcgctt
     6361 tgaaataatt cgccacgcta ccgatcgcat caaccggatc ccacaggttg atatgcccgt
     6421 cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt ccgtagccca
     6481 tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg cgcgccatca
     6541 gcaggaaggt ttccagctcg ccagagaaat actccgcgcg gcgtgggtag ttaaatgaca
     6601 gcgttgccag cgcatcgagg atgcgagttt tccccatcac gcgcccccag cgggtttcaa
     6661 cgccgataat cccgacgata atttccggcg gtacgccata cacctgccac gcgcgattca
     6721 acgcatcttc atactgattc cagaaaacca caccgttctg cacattgtcc ggcgtaataa
     6781 attttttgcg ataacgcagc catgcgccgt tcggacctga tggcggtttc accgatgtgg
     6841 ttggtgcctg gttatccatc agccgcagta ccgaatccag acgtttcgcc tgggagagaa
     6901 tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattctgtcg atgaactgct
     6961 gggcattcgg gttattagcg aaatcgccgc ccatctgcat cacattgtgc tgcggctcaa
     7021 gcaggaagcc gccagacggc gttccggtgg tcgtttcagt ctcagtaggt tttggcttgc
     7081 tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga cgcttgaaca
     7141 tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc acgccccgca
     7201 caaggaagcg gtagtcactg cccgatacgg actttacata actcaactca ttctcctcgc
     7261 tatcctttta ctcaaacttt caaattaaaa tatttatctt tcattttgcg atcaaaataa
     7321 cactttaaat ctttcaatct gattagatta ggttgccgtt tggtaataaa acaataaatc
     7381 ctgaaggaga gaacaatgat agaaaccatt actcatggtg cagagtggtt tatcgggctg
     7441 ttccaaaagg gcggagaggt gtttaccggg atggtgaccg gcattcttcc gctgttgatt
     7501 agcctgctgg ttatcatgaa cgcactgatt aattttatcg gtcagcatcg tattgaacgt
     7561 tttgctcaac gttgcgccgg taaccctgtt tcccgttacc tactgttacc gtgcattggc
     7621 acgtttgtct tttgcaatcc gatgacctta agcctgggtc gctttatgcc ggaaaagtac
     7681 aaacccagct actacgcggc ggcctcttat agctgccact caatgaatgg cctcttcccc
     7741 catatcaacc ctggcgaact gtttgttatc ttggcattgc cagcggtctg acaacgctga
     7801 acctgccact tggcccactg gcggtgagct atctgctggt tggtctggtc accaacttct
     7861 tccgcggctg ggtgaccgat ctgaccaccg ctatttttga gaaaaagatg ggcattcaac
     7921 ttgaacaaaa agttcatctg gcaggagcaa catcatgacg catattcgga tcgaaaaagg
     7981 aacggggggc tggggcggcc cgcttgagct ggaaaccacg ccgggcaaaa aaatcgtcta
     8041 tatcaccccg ccggtacccg gcctgcgatt gtcgacaaac tggcacagct tactggctgg
     8101 caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta
     8161 atcgactgtg gcggcacatt acgctgcggc atttatccga aacggcgtat tcccaccatt
     8221 aatatccact cgacgggcaa gtccggtcca ctggcgcagt acattgtgga agatatttat
     8281 gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc acaaccctct
     8341 tccgtgggcc gtgactatga caccagcaag aaaatcaccg aacaaagcga tggtttactg
     8401 gcgaaggtgg gaatgggtat gggttctgcc gttgccgtgt tgtttcaatc tggtcgtgac
     8461 accatcgaca ctgtattaaa aaccattctt ccgtttatgg cattcgtctc ggcgctcatt
     8521 ggcatcatta tggcttctgg ccttggtgac tggattgccc acggccttgc tccgctggcg
     8581 agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccgct gctttcacct
     8641 ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg cgtgcagatt
     8701 ggtctcggca atattccgcc gcatctggct ttacctgcac tgtttgccat caacgcgcag
     8761 gcggcctgcg acttcattcc ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt
     8821 cgcgtcggtg tcccttctgt actggtgagc cgctttttaa ccggcgcgcc gactgtactg
     8881 atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga ccgttattta
     8941 tcagaccacc atcacccgta tcggcgcgag tgccactgac gccctcagcg accagatgct
     9001 catcaccttt cgtgaaggcg cgccagcgga cctcgaagag tattgcttca ttcattgcca
     9061 cggcgagttg aaaggtgcac tccatcccgg tttgcaattt acgctcgggc agcatcgcta
     9121 tccggtgacc gctgttggca gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac
     9181 cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg tggcaggccc
     9241 tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta aggagtaaaa
     9301 aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca
     9361 cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc
     9421 aaatgtggca caagaaatta acgccgaata tggtgaagga acagcgtatg gttttggtgc
     9481 tgacgccact agcgagcaaa gcgttctggc gctctctcgt ggggtagatg aaatctttgt
     9541 tcgtgtggat ttactggtct acagcgccgg aattgcaaaa gcagccttta tcagcgactt
     9601 ccagctcggc gattttgacc gttcgctaca ggtgaatctg gtgggttatt tcctgtgtgc
     9661 gcgtgaattt tcgcgtttga tgatccgcga tgggattcag ggacgcatta ttcagatcaa
     9721 ctcgaaatcc ggcaaagtgg gcagcaaaca caattctggt tatagcgccg cgaaatttgg
     9781 tggcgtaggg ctgactcaat cactggcgct ggatctggcg gagtacggca ttacggtgca
     9841 ttcactgatg ctcggtaacc tgctgaaatc gccgatgttc cagtcgctgt tgccacaata
     9901 tgccaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg acaaagtacc
     9961 gctcaaacgc ggctgcgatt atcaggatgt gctgaatatg ctgctgttct acgccagtcc
    10021 taaggcgtcg tactgtaccg gacagtcgat caatgtcacc ggcggtcagg tgatgttctg
    10081 atcaacagcg gagatccgtt aaggatctcc gtgagactaa tagaatgcct gatgcgctac
    10141 gctcatcagg catacaggac ttccgccact acattaagga aaagttatgg tatccgcact
    10201 catcactgtc gccgttatcg cctggtgtgc gcaactggcc ttaggcggct ggcaaatttc
    10261 tcgttttaac cgtgccttcg acacactatg ccagcaaggg cgggttggcg tgggccgttc
    10321 ccgcgggcgc tttaaaccgc gggtcgtggt cgccatcgcc ctggacgatc agcagcgcat
    10381 cgccgacacc ttgtttatga aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc
    10441 aattaccggt atgcatgcgg gtgatttaca gcccgatgtg atctttcccc atgatccact
    10501 atcacagaat gctctatcat tggcgcttaa actgaaacgt ggataatttc gttgtgaatg
    10561 ttacttgctt gcgaagttat cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc
    10621 tcgtcagcgt caagccgcca ttctggagta tctgcaaaag cagggaaaat gctcggttga
    10681 agaattggcg caatattttg acaccacagg cacaactatt cgcaaagatc tggtcattct
    10741 ggaacatgcc ggaaccgtca ttcgtactta tggcggagtg gtgttgaata aagaggaatc
    10801 cgatccgcct atcgatcata aaacactcat caacacccac aagaaagagc tgatagcaga
    10861 agctgccgtt ggttttattc atgatggcga ttcgatcatt cttgatgctg gcagtaccgt
    10921 tttgcagatg gtgcctctgc tctcgcgctt taataacatc acggtgatga ccaacagcct
    10981 gcatatcgtc aatgcgttat ctgaactgga taatgaacaa actatcctga tgccaggcgg
    11041 aacgtttcgt aaaaaatcgg cctcatttca cgggcagctg gcagagaatg ccttcgagca
    11101 tttcaccttc gacaaattat tcatgggtac cgacggcatc gatctcaatg cgggcgtaac
    11161 caccttcaac gaggtctata ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat
    11221 tttgatggcg gactcatcaa agtttggccg taaaagcccc aacgtagttt gcagtcttga
    11281 aagcgtcgat aagctgatta ccgacgcaga tatcgatccg gcgtttcgtc aggcgctgga
    11341 agagaaaggg atcgatgtga tcataaccgg agagagcaat gagtgaagca ctactgaacg
    11401 cgggacgtca gacgttaatg ctggaattgc aggaagcaag ccgtttaccg gaacgtctgg
    11461 gcgatgattt tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa gtggtggttt
    11521 cgggaattgg caaatcgggc cacattggta agaaaatcgc cgcaacgctt gccagtaccg
    11581 gcacaccggc tttttttgtc catccggcag aagcgctgca tggcgatctg gggatgatcg
    11641 aaagccgcga tgtgatgctg tttatctctt actccggtgg ggcgaaggaa ctggatctga
    11701 ttattccgcg tctggaagac aaatctatcg cgctgctggc gatgaccggc aaaccgacgt
    11761 caccgctggg cctggcggcg aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc
    11821 cgatgcacct tgcgccgacc tccagcaccg tcaataccct gatgatgggt gacgcgctgg
    11881 cgatggcggt catgcaggcg cgcggattta atgaagaaga ttttgcccgc tcccaccctg
    11941 ctggagcact gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc cgtgacgatg
    12001 ccatcccaca ggtggcgtta accgccagcg tgatggatgc tatgctggaa ctcagccgca
    12061 ccggtctggg gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg
    12121 acggcgattt acgtcgctgg ctggttggcg gcggcgcact caccacgcca gtcaatgaag
    12181 cgatgacggt cggcggcacc acgttgcaat cgcaaagtcg cgccatcgac gccaaagaga
    12241 tcctgatgaa gcgcaaaatc actgccgcac cggtggtgga tgaaaacggc aaactcaccg
    12301 gcgcaataaa cctgcaggat ttctatcagg ccgggattat ttaatccttc aatcccagac
    12361 gtttcgccag ccgatgcagg ttggcgacgt cggtttccag catccgcgcg caggcagccc
    12421 agttgtgatg attttgtgcc agtgcctgac gaatagtttc acgctggaac gcttctgtcg
    12481 cttcacgcag gttttgctta acaacgggca ccgccgccac ttctggcggc ggcaacgtca
    12541 cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc atcgccgctg cgggtggctc
    12601 tcgccagcac taccgcccga tgaatagcat gttccagttc gcgcacgttt cccggaaaac
    12661 tgtagtgttg cagtaaattt cgcgccccgg cacttaatac cacgcgggag agcccctgcc
    12721 gcaaacgaca ctgctcgcag aaataccccg ccagcagaat gacatcatcg ccccgctcac
    12781 gcagcggcgg caccgaaagt ggaaacacac tcaggcgatg aaacaaatcg gcgcggaatc
    12841 gccctgccag cacctcttcg cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga
    12901 cccgcaaact acggtcatcg ccaacgcgct gaatatcgcc atactgcaac accctcagca
    12961 gcttggcctg caatgccaac gacaactcgc cgatctcatc gagaaacagc gtgccgttat
    13021 ccgccatttc aaacttccca ctgcggttac tgatagcgcc agtaaacgct cctttcacat
    13081 gcccgaacaa ctcactttcc gccacacttt ccggcagtgc ggcacagttg agatagacca
    13141 gcggattcac cgcccgtggc gaggcctcat gaatcgcttt cgccaccagc tccttaccgg
    13201 ttcccgtctc accgctgatc aggacgttga gatcggacgc cgccacaatc tcaatctctt
    13261 ttttcagttg cgtcatgcct ggcgacagac cgatcatctg cgtctgtttc accgcttcaa
    13321 atggagcggc atcgcccggc agcatattct ggctttccag ttgttcaatc agcaacgcat
    13381 tgcttaacgc tcccgccgcc agcgcggcaa tcaggcgtaa ctcttcgtcg ctgaaaacat
    13441 cgaactgatc gggctgcatc ccgtcgagcg tcaatgcgcc gatcaggttt tgcccggcaa
    13501 acagcggcag accaacgcag gcgtgaacct tcagactctc ctgccctgga atcaaaccgt
    13561 catagggatc gggcaattcg ctgtctgcgg gaaagcgcac cacatcaccc gcgcgggcaa
    13621 tcgcttccag ccgtggatga ccttccagcg caaagcgtct accgagtaca tccttcgcca
    13681 gcccgtcgat ggcaagcgga ataaactgcc gcgagtcgta acgtagcaac gcagacgcat
    13741 cgcactccag cacctgacgc agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa
    13801 tcccacgctg caattcgatg gcgatattcg ccagcacatc aacggaaaaa ctcatctttg
    13861 cctcactgtc aatttgacta tagatattgt catatcgacc attggattga tagtcatttt
    13921 gactactcat taatgagcat aattttattt atagagtaaa aacaatcaga taaaaaactg
    13981 gcacgcaatc tgcaattagc aagacatctt tttagaacac gctgaataaa ttgaggttgc
    14041 tatgtctatt gtggtgaaaa ataacattca ttgggttggt caacgtgact gggaagtacg
    14101 agattttcac ggcacggaat ataaaacgct gcgcggcagc agctacaaca gctacctcat
    14161 ccgcgaagaa aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt
    14221 gcagaacctg cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga ttaaccatgc
    14281 agaagaggac cacgccgggg cgctgaccga actgatggca caaattcccg atacaccgat
    14341 ctactgtaca gccaacgcta tcgactcgat aaatggtcat caccatcatc cggagtggaa
    14401 ttttaatgtg gtgaaaactg gcgacacgct ggatatcggc aacggcaaac agctcatttt
    14461 tgtcgaaaca ccaatgctgc actggccgga cagcatgatg acttacctga caggcgacgc
    14521 ggtgctgttc agtaacgatg ctttcggtca gcactactgc gacgagcatc tgttcaacga
    14581 tgaagtggat cagacggagc tttttgagca gtgccagcgt tactacgcca atatccttac
    14641 gccgttcagc cgtctggtaa cgccgaaaat taccgagatc ctgggcttta acttaccagt
    14701 cgatatgata gcgacctctc acggcgtggt atggcgcgat aacccgacgc aaattgtcga
    14761 gctgtacctg aaatgggcgg ctgattatca ggaagacaga atcaccattt tctacgacac
    14821 catgtcgaat aacacccgca tgatggctga cgctatcgcc caggggattg cggaaaccga
    14881 cccacgcgtg gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac
    14941 caatgtcttc cgctcaaaag gcgtgctggt cggcacttcg acgatgaata acgtgatgat
    15001 gccgaaaatc gccgggctgg tggaggagat gaccggatta cgcttccgta acaaacgcgc
    15061 cagtgctttc ggctctcacg gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct
    15121 gcaggatgcg ggtttcgaaa tgtcgcttag cctgaaagcg aaatggcgac cagaccagga
    15181 cgctctggag ttatgccgtg aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc
    15241 gctgccgcag agcacggtga atacggtagt taaagaagaa acctctgcca ccacgacggc
    15301 tgacctcggc ccacggatgc agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg
    15361 cgagccaatg caggacgttg cgccaggaac gccgtggagt gaagtcccgg ataacttcct
    15421 gtgcccggaa tgctccctcg gcaaagacgt ctttgacgaa ctggcatcgg aggcaaaatg
    15481 agcaacggca ttgtgatcat tggttcgggc ttcgccgccc gccaactggt gaaaaatatt
    15541 cgcaaacagg acgccagtat tccattaacc ctgattgccg ccgacagtat ggatgagtac
    15601 aacaaacctg acctcagcca tgttatcagt caggggcaac gtgccgacga ccttacccgc
    15661 cagacggcgg gtgaatttgc cgagcagttt aatctgctcc tgtttccgca aacctgggtg
    15721 acggatatcg atgccgaagc ccgtgtggtg aaaagccaga ataatcagtg gcaatacgac
    15781 aagctggtgc tggcaaccgg tgccagcgcc tttgtcccgc cagtgcctgg gcgtgagtta
    15841 atgctgacgt taaatagtca gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc
    15901 cgacgcgtgt tgattgttgg cggtggtttg attggtagcg aactggcgat ggatttttgt
    15961 cgtgcaggca aaatggttac gctaatcgac aacgctgcca gtattctggc gtcgttaatg
    16021 ccaccggaag taagcagtcg cttgcagcat cggttgacgg agatgggcgt tcatctgctg
    16081 ttgaaatctc agttgcaggg gctggaaaaa acggattctg gcattctggc aacgctggac
    16141 caccagcgca gcatcgaagt ggatgcggta attgccgcca ccggactgcg cccggaaacc
    16201 gccctggcac gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga tagttatctg
    16261 caaaccagta atgccgatat ttacgcgctg ggcgattgcg cggaaattaa cggtcaggta
    16321 ttgccgttcc tgcagccgat tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc
    16381 aataacacgc cgctgaaact accggcgatg ctggtgaaaa tcaaaacgcc ggaattaccg
    16441 ctgcatctgg caggcgaaac ccagcgtcag gatttacgct ggcagattaa taccgaacgc
    16501 cagggaatgg tggcgcgcgg tgttgacgat gcagaccaac ttcgcgcctt tgtggtcagt
    16561 gaggatcgga tgaaagaggc atttggattg ttgaaaacgt tgccggtgta ggtgggctac
    16621 tgtgcctaaa atgtcggatg cgacgctggc gcgtcttatc cgacctacgg ggacgcatgt
    16681 gtaggccgga taaggcgttt acgccgcatc cggcaatggt gtccaaatgc aacacgtttt
    16741 atccgttctg gacttcaccc gctaaccaac gcgccgcagc aataaccccc tgccccagag
    16801 acaaaccgcc atcacccgcc ggtaaactct gtggaaacag caatgtgaaa tcagcgagat
    16861 aatgcgccag acgtgcacgc agcaaacggt tatgaataac cccgccgcta aataccagcg
    16921 tagtgatacc acgcatcgtg gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg
    16981 catcatgaaa cgcccacgcg cgttgattaa ccggtgcctg ccagttcagc cactgctgcc
    17041 agaaagtggc gagatccagt tgattgtcca cccgcggcat cgtcaccgga tgcgtcactc
    17101 cgtcgcatga ggctgctagc gcctccagag cacaagccgc ttcaccttca taacttaacg
    17161 tggctggcgc acagcccagt gccgccgcca cagcatcgaa caaacgccca cacgatgacg
    17221 ccagcggcgc gttaattcca cgctcaatgg cccgcgccag tacgctccag ttttgctgtt
    17281 gcacacttgc cgtttcaggg taattctgcc actccggcac aaagcgcagg cactgcgcca
    17341 gcaggttacg ccacggctgc ttcgccgcca aatcgccacc cggaagcgcc actgcaggca
    17401 agccgcccag gtgctcacat tcgcgatagt tcacccgcag gcactcgccg ccccacaaag
    17461 cgccgttctc ccccataccg ataccgtcga gcgtcaaagc aatgacatca ccgccatcca
    17521 gcggccactg atgctctgcc agacacgccg ctgcgtgggc atgatgatgc agcaccgttt
    17581 gcgtcggcag attcatttcg ctcgcccact ggcaggagac atagcccgga tgtgcgtcat
    17641 gcacaacgta ttgcggagtg aaattgtaga tgttttgcat caggcgtaac gcttcgcgcc
    17701 actgcgtctg gatgccatcg tcacttaaat cgcccagatg ctggctcaac accacttgtt
    17761 caccgcgcac caggcagaag gtatttttca gatccgcgcc gagacacagt acaggcggaa
    17821 catttttaaa gcccggaggc aaagccagcg catccggcac atacccccgc gaacggcgca
    17881 gcatttcgcc gctttcgcgc accaccgaat catccatccg ctgcacaatg tcgcggttgt
    17941 gtattaagaa tccgtcggca atgccctgca aatcctccag cgcctgttcg ttgctgatag
    18001 ccggtggttt accgctcagg ttgccagagg tcatcaccag cgggcattgt agttcctgta
    18061 acagcaaatg ctgaagcgga ttcgcaggca acattacccc gacttcgtta aggccagggg
    18121 cgatatcatc acaaagctca ggaacgtatt ttttatccac cagcacaatc ggcgcggcgg
    18181 gcgtggtaag caactggcgc gcagcgtctg gtaaaccgtc agccactggc aacatgactg
    18241 ccagcggttt cgccgggcga tgtttgcgcg cccgaagtgt cgccaccgcg ttactgttac
    18301 gtgcatcgca ggcaagatga aatccgccaa tccctttgat ggcgacaatg ttgcccattt
    18361 ttaactgtgc gatagccgcc tgtaatgccg cctcttgttc tgcatgttca ccatgactta
    18421 cccattcaag atacgggcca cactccgggc aggccaccgg ctgggcgtgg aagcgacgat
    18481 cgagcgggtc gcggtactct ttatcacagg ctggacacag cggaaacgcc gccatcacgg
    18541 taaacgggcg atcgtaaggc atggcgcgaa taatggtgaa gcgcgggccg cagtgggtac
    18601 agttgataaa tggataacga taacgccgtt cgcctggggt attcatttcg gcaaggcaag
    18661 cagggcaagt ggcggcatcg ggaacaattt gcgtattcat ggcgccgccc gcgctctggc
    18721 ggatggtgaa ctcagtgggc agttgtgacc agataaacgg ttcacgctca acgctatcaa
    18781 tacgcgccag cggcgggcag tgctgatgca attgaacaag aaacgtttcc gggtcttcca
    18841 gcagccggac ttctacgcca tcgccgtcat tacagacatc gccgtgaaga tttaattgct
    18901 gtgccagctg ccagacaaac ggacgaaaac cgacgccctg cactttgcct cgaatacgca
    18961 gttggacacc gcaagatgtg ttttttgcca ttgagttatt cccgccatca tgaattgcgt
    19021 aacccgtcca gccggacacg acagcgtcgc atccggcagt cacaggtcgg cgataccgcc
    19081 cgctccgtat tctacgaata tttccgggaa ttcctttgat gccagaacag ttctgtaaga
    19141 tttttagaac atcagcgccg tacggcggcg tttttctgcg ctcagttgtt caagtttatt
    19201 gcgatcgaca caaattagcg catgggtcgg gcaagccgcc atacacgccg ggccgtcttc
    19261 acgatggttg cacaggtcgc atttattggc ttcggctttg tcagcccgta cattcagccc
    19321 tgcgccgctg ttgcgaatca ccggacgtac caccacttcc atcgcgccat acgggcaagc
    19381 cacaacgcag gttttgcaac caatgcaacg ttcctgcatc acatgaacaa accctttatc
    19441 acggctgata gcaccattcg ggcagacgtt agcgcacggt gcatcttcac actgacggca
    19501 aactgtcgcc gtggaaatgt tcacaccttt aatgacatgg atacgcggta aaaatgtttc
    19561 cggggtcagc gatgcacagt cctgattttc ctgatgagaa accacgcacg ccacttcaca
    19621 ggtacggcaa ccaatacatt tactcgcgtc agcaatgatg aaacggttca tcaaattctc
    19681 cagcaatgac agttaatgcg ccgatacatt cacaaatcat gccagttttt aatttgctgt
    19741 tatttaagga aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga
    19801 tgacatgtga tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg cggatcagtt
    19861 ttccgctgaa ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc
    19921 caataatttc ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga
    19981 tatagggggc aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta
    20041 cgccgcgctt gtgtaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta
    20101 acgcgctaaa tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg
    20161 gcgtccattt accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg
    20221 cgtctttata tccggcaaga cgttcaacgc tggtggggga atccatcgag ccggtaagaa
    20281 aagcaatctc ctgatgcccg gcgtttatca actctgccac ggcgttaaag ctggtctgtt
    20341 tatgatcgca ccagacgcta tggctgctat ttttgcgcag gcggcgatta agcaccatta
    20401 tcggctgact gtgcgcgtca atgatgtcat cgatctcatc cacacttaaa aaacgcgggt
    20461 aaatcataat ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt
    20521 cggcgctgtg tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg
    20581 cggcatgaaa cagtaattca ctaaaataaa tgccgtggta aagcgtgtta gtcactacca
    20641 gccccagcgt ctgggtactt ttcgccgaca gattgcgcgc cagcaagttt ggacggtaac
    20701 cgctctcttc taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac
    20761 ctgaaagcac gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca
    20821 tcgtcgtcat cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttacc
    20881 gattgtcctt gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa
    20941 aatgaacaat taactctctt gcttatttgg ttacaactat tcatgatttt gtgaaaccgg
    21001 tttcttaatt ccgtttcagc atcggcattt ttccgtcacg tcgactgata acaactacat
    21061 cttccctact gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct
    21121 cggtgatagc ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc
    21181 gtttgcgctt tgttatcaaa gatgatgcgc ttatcgacag cccgacgtta aaaaccatcc
    21241 ccggcgtgct cggcgtggta cgtagtgaca accagtgtca ggtgattatt ggcaataccg
    21301 tttcacaagc ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgccctgc
    21361 ccgtgggtaa acccaaactc acgctgcgtc gcattggtgc ggggatcctc gatgcgctga
    21421 tcggcaccat gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg
    21481 cgatgatcct cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga
    21541 atgtgattgg tgacggtgct ttcttcttcc tgccgctgat ggtcgcggct tcggccgcca
    21601 tcaaatttaa aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga
    21661 gttttattga actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc
    21721 cagtcaccgc ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc
    21781 tgtcatatat cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca
    21841 agccaatgct gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg
    21901 gtatctggat cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct
    21961 ggctttcagt cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc
    22021 gcgtctttac gctaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca
    22081 tgccgtcaga gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga
    22141 aaacgaaaaa cccggaactg cgccagactg cgctggctgc ggcggcatca gccattatgg
    22201 cggggatttc cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg
    22261 ccagtcttat cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc
    22321 actcaatggc agcgccgggg ctatttacca gcgtgcagtt cttcgatccg gcgaatccaa
    22381 tgagcatcgt ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca
    22441 cactgttgct cggctttgag gatattcctg ttgaggaagc ggctgccgag gcgcgaaagc
    22501 atcagagcgc acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc
    22561 agtatttcca gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc
    22621 gttccgtgaa ggtggcaaag gactgacaac ggtcgatatg atcccacacg gcgagcatcg
    22681 aatggcggtg aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc
    22741 cagccatgag gcgacgggtt tttatcatcg ttataaagaa gatatcgccc tgatggcaga
    22801 gatgggattc aaagtgttcc gtacctcaat tgcctggagc cgccccttcc cgcagggtga
    22861 tgaactgacg cccaaccagc agggcattgc tttttaccgt tctgtctttg aagagtgtaa
    22921 aaagtacggt atcgaaccgc tggtcacatt gtgccacttc gatgtgccga tgcagctggt
    22981 caccgaatat ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgccag
    23041 gacctgcttt gaagcatttg atggtctggt gaaatactgg ctaaccttca atgaaatcaa
    23101 cattatgttg catagcccgt tctccggcgc ggggctggtg tttgaagaag gtgaaaatca
    23161 ggatcaggtg aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa
    23221 aatcgcccat gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt
    23281 ctacccttac agttgcaagc cggaagatgt ctgggcggcg ctggagaaag atcgggaaaa
    23341 cctgtttttt atcgatgtgc aggcgcgggg cgcgtatccg gcttactctg ctcgcgtatt
    23401 ccgcgaaaaa ggggtaacca tcgacaaagc accgggcgat gatgagatcc tgaaaaacac
    23461 cgtcgatttt gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacac
    23521 caacaatagc agtgcggcga acgtagtgaa atcgctgcgt aacccgtatc tacaggtgag
    23581 cgactggggc tggggaattg atccactcgg tctgcgtatc accatgaaca tgatgtacga
    23641 ccgctatcag aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc
    23701 tgccaatggc gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc
    23761 aatgggcgaa gcgattgcag atggcattcc gctgatgggc tacaccacat ggggctgtat
    23821 tgatttagtt tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctttgttga
    23881 ccgtgacgac gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta
    23941 taaaaaagtg attgccagta atggggaaga tttagagtag gtaacctact gtcgcactaa
    24001 ccaggaaggc ccgataagcg ttgcgcatcg ggcaatctgg ggtttgctat cagttctgat
    24061 tccctcatca agaacatccc tgtcctgatt acttattgaa aaactactct tcttccaccg
    24121 ctaactgcgc gaagccgccg ttcccttccc agccttccag tcgttgataa acggtttcta
    24181 ccgcatcttt aatcggctgg gtcatcgggt agtaaaagcc gacgatatcc ggctggatgc
    24241 cgaggaaaat cacttcgcca atatcttctt tcaactgatc gataaggtag ttcaacggca
    24301 tgttatgggt agtcatcata aacatctcgg cgatatcatc cgggtcgatg atgcggattt
    24361 cgccggggtt tagccccata tccgtggcgt cgacaatcag cagccgtgtc gggcgcagtt
    24421 cgcggatagc gacaatgtcg ttttccggtg cgctaccgcc gtcaatcacc acccagttac
    24481 ctttcggcgc ggcggcgcac ttttccgcca gcagcggacc tgcgccatca tcacccatca
    24541 tgctattgcc aacacagagt aaaacgtcag tcacgcagtc tcctcaccat caaatagatg
    24601 gcgttttcct gatgaatatc atgcagcata cttaacagca ttgtactcca ttcctgctgc
    24661 tgcgaagttt gcgctgcccg ggctttatcg aatgcattgg ccagcatcgg cacatggtta
    24721 atgtcgatga cgatctcacc atatttcggc acaccttcca ttttgcggcg ggcttcgctg
    24781 cccgcctcca gagtggcaat ccatgccaga tattcatccc acgggcaggt gagcgccgct
    24841 tccaggcaat cgataacccc aaggtggtga ccaatcgcca ggctgtaata gaccacctgc
    24901 tgcgcctcgg cgggcgtggc atcgttctca tcaataaatt tacggctcag ttgactgaac
    24961 accacctttt cactcatcgg atacgcgcct cttcaacaac atgattcaga tggctgacaa
    25021 tctcgttcag acgcggatcg ttttccgctt ccagccagcg tgcaacctgt tcttcgccct
    25081 gccctaactg tgtaaggtaa tcatcggcaa tctgacgacc gtaacgataa cccgccagtc
    25141 gacgtgcttc gcgatccact ttcacacgca gcggctgcac catatcgcca tgcaggatct
    25201 ccgccggttg ttcatcctgc tcacccggcc cacgggcgtg aattttctgc tccagcaggc
    25261 cgagcgccat tgcaaagccg tacagcgtgg cggctggcgt tggcgggcag ccagggatat
    25321 aaacatccac cgggacgatt ttatccgtac cgccccacac gcagtagaga tcgtggaaga
    25381 tcccgccgct gttaccgcag gcaccgtagg agatacaaat tttcgggtcc ggcgcggact
    25441 gccacgcacg cagcgcaggg gatcgcattg cacgggtgac cgcgccggta aacagtaaaa
    25501 tatccgcatg acgcggtgaa ggaacgactt taatgccgaa gcgttctgca tcgaacagcg
    25561 gcgacaaagt ggcgaaaatt tcgatttcgc agccgttgca gccgccgcag tccacgcggt
    25621 aaacgtaggc agaacgtttg atttttttca gtaacgacgc cttcatgctg gcgatggatt
    25681 catccaccgt catcgggacc ggaatgccgt tggcgtcacg ggggcctaat aaattgctca
    25741 tcagatggcc tctttcatat ggcgagtcag ttcaatacgg tcggacggca ccaggcattt
    25801 ctggcgctta cattccgggc aagtctcaaa gctttcgcgg tggttttccg cgcggctgtc
    25861 gccattgtgc ttaagcagcg caatggcgta gtcgatctct ttctggacgg cgaaaggacg
    25921 attgcagacg cggcagttgc acagcgcgaa gcgggactgt tgcaggaagt cttctttctt
    25981 ccacaccgcc agttcgtact cttgcgacag tttgattgcc gccgtcgggc agacttcttc
    26041 gcagcgtccg cagaagatgc agcgcccgag attgaactcc cacgccagct caccggtggc
    26101 gaggtcagtt tcaaccgtta aggcgtttga cgggcaggca ttgacgcagg ccgcgcagcc
    26161 gatgcactgc tgcgggttct gctctggctt accacggaag tttttatcaa ccgcaatcgg
    26221 ttccagcgga taagacgagg tcgccgtgcc ggttttgatg acttttttga taaaggtaaa
    26281 catggcgatt ccttatttca gcggcgagtt tttacgctca atgctgtaac gctcgagttc
    26341 tttgtacggc accactttgc tctttttctt acgcacatcg accacggtca tgcggtcggt
    26401 acaggagtag caagggtcca gactaccgat aatcagcggc gcatcagaaa cggtgttgcc
    26461 gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc gcggcacggc agcgccagcg
    26521 gtacagcttc tggttgtcgc cggtcatgct ccagtggata tcatcgccgc gcggcgcttc
    26581 ggcaaagccc agcgcaaaac ggtgcggaat gtaggtaaag ccttccacca tcagcgggcc
    26641 gcccggcagg ttatccagac cgtagtcgat catgttcagc gcggtataga cttcgttaat
    26701 acgcactttc agacgcgaaa taacgtcgca gccctgctcg ctgtggactt ccattggcag
    26761 caggccataa ccaacaaacg ggtgatcggc gcgggtatcg cgggcgtgac cgctggcacg
    26821 gaccatcggg ccgacgttac tgaagtcgcg agcgatttcc gggtccagac gaccaatgcc
    26881 gacagtgcgc tgttccatgt tcggcgtact cagcagcaca tccaccagct cctgcacttc
    26941 acgacgcatc tgttgcgcca gttgacgggt ctggatcatg tcatctttca gcagatcgcg
    27001 acgaatcccg ccgatcaggt tcaggccgta ggttttacgc gcaccggtaa ggatctctgc
    27061 cattttcatg gaggtttcac gcacgcggaa gaactgcata aagccggagt caaagccggt
    27121 aaagtgacag gcgaggccga ggttgagcag atgcgagtgc agacgttcta cctccagcag
    27181 aatggcgcgg atcatctgcg cacgttctgg cacctgaata cccatcgcgt tttccaccga
    27241 cgtggtgtag gcggtgctgt gagcaaaacc gcagatcccg cacacacggt cagagaggaa
    27301 ggtcacttcg ttataaccca tgcgggtttc tgccagtttt tccatgccgc gatggacata
    27361 gaacaggcgg tagtcggcgt cgataatgtt ttcgccatcg acgaacagac ggaagtggcc
    27421 cggttcgtca gaagtgacgt gcagcggacc aatcggcacg acgttgtttt tcttgtcgcc
    27481 cagttcgttg atgaactcgt aggtttcagc atcggtggtc ggtgccggac gctgacgata
    27541 atccatgctg tctttacgca gcggataaag ttcatccggc cagtcatccg gcagcaccag
    27601 acgacgttca tccggcaaac caaccggaat caaaccgtac atatcacgta cttcacgctc
    27661 gccccacacc gcagccggaa cgcgcggcgt cacggaagga tactccggtt tgttggcatc
    27721 aacttcgacg cgaaccgtta cccagcactt agtccccttc tccatcgaca gcacgtagta
    27781 aacggcgtaa tgaccattca gtttgcgttc gtcgttacca aaaagcaccg acagccagcc
    27841 cccctgcttg tagtagagaa actccaccac ttccggcaga tagttcacct ttacggtgac
    27901 agtcagctga tctttggtct gccaggcgtg gtccagcacg acgcccggaa atgcctcatt
    27961 cagcgcggcg agataatgtt gacctaattt ttcttcagac atgctcaaac tctctttaat
    28021 cacgccgcca gcaaggagac gaacgctaaa aatgcaaagc caaacccagc ccaggtaatg
    28081 cgcggagtaa tatcaagacg cagacgggcc atgctgtttt cgaacagcgc gataaccagg
    28141 acgccgacca ccagtttgac gatggcaatc accaacgcca gcagcagccc accgacggtg
    28201 aaggtttcca tttgtcccca cggaataaac accccgacga acatctgcag caccaccaac
    28261 tgtttcaggc tgatacccca tttcatgacg ccaaagccgc tgccgctgta ctcagagagc
    28321 ggaccttcct gcaactcctg ctcggcttcc gccaggtcga acggcagttt gcccatttcg
    28381 ataaaagtgg cgaacgcaca ggcacaaagc gccagcacca gcgggatgct ctggctcagc
    28441 ggccagtgat aaacggtgtc ggtgatgttg ctgatgttgg tggaactggc aacctgtgcg
    28501 gcaacccaca gaccaagcag cagcatcggt tcgaccagca cgccaagcat cgcttcacgg
    28561 ctcgcgccga tagcggtaaa cggactaccg gtatccagac cagaaatggc aaagaagaaa
    28621 cgcgcgatgg caaagagata cagtaaggtg atcaaatcac ccagttgcgg cagcggagaa
    28681 ccgacggtca ccaccggcag cgcagtagcg atagtcagca tgacgcccac catcacatac
    28741 ggcgtcaggc ggaacaccca gccggaggca tccgggccaa cgctctggcg acccagcagt
    28801 ttgataatgt cgcgatactc ctgcaacacg cccggcccgc gacggttatg caagcgggcg
    28861 cgcgccacgc gggtaatacc ggagagcagc ggcgcaacgg caaatagcac cagcgcctga
    28921 attaacggat ataaaacact cattctcagg ctcctcgtga aacaataatc accaccagca
    28981 ccgccagttc aaccagcgcc atccggcgga acagcaacgc actgccttcg cactgccagc
    29041 ccggcaccag cgacaccgga ttcagccatt tacgcagttt cagcaccggc gcaaacgcct
    29101 gtttcaccgg catggcaaaa ccgtgggcgg taattaccat tgatttttca tggtcgtagc
    29161 cgcacaccca ggccgcgccg cgggaacgcg atggcaaacg atcgcctttg caaatcgcca
    29221 taatgatgaa tggcagcagc gggcaggcaa tcagcagcaa cgtgatcatc ggttgagaaa
    29281 cggtggtgtt agcaggttcc agcggcagag gtacagcagc agagagcatc ggcagcagcc
    29341 acggcgcagc aacaccgcca attacgcagc aaatcgccag tgccactacg cttacgctca
    29401 tcaggagcgg tgcacaggtg gcgttttcgg cttctttggt acgcggcgcg ccgaggaacg
    29461 tgacgccata gactttcgcc atacacatca ccgccagcgc accggtaatt gccagaccca
    29521 cagcgagcag cggccccagc agacgggcaa caaacgcgcc actattgctc agtttgaaga
    29581 aggattgata gataacccat tccccggcaa aaccgttcag cggcggcagt gcagccattg
    29641 ccatcagccc gactaacata gcgatggaga taaccggcat tttcttgcca ataccaccga
    29701 gtttttcgat atcgcgatga ccggtacgga accagacgct ccccgccccg aggaacagta
    29761 cgcttttgaa caggctatgg ttaagcagat ggtacagacc accgaccagg ccaagagcaa
    29821 tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc gcccagcccc agcaggataa
    29881 tgccgatatt ttccagggtg tggtaagcca gtagacgctg gatattatgt tccatcaacg
    29941 catacagacc gccgacaaac gcggtgatca taccgagcac cagcagcgcg atcccccacc
    30001 acagcggtgc attaccgccc agcagtgaca gggttaaaat gcccagcagg ccaattttca
    30061 tgactaccgt agaaaacaac gcggcagctg gcgcagaggc gttcgcatgt gcctgcggca
    30121 cccagccgtg cagcggaata atcccggcca gcaggccaaa gccaatcaca ctgagcagcc
    30181 agatatcgga accgagcggc agctgttgca tacgcatatc cagcaggcgc agatccagcg
    30241 tgccgtaacg ctgccacagc agccagcaag caatcgccag cagcagagtg ccaagacgcc
    30301 ccagcgcaaa ccacagtttg ccctctttgc tgttgctggt gaggaacacc gcacacaagg
    30361 ccatgacttc ggccattacc acgaacatgc cgaggttgct ggcaatgacg gcgcagacgg
    30421 cggcagccat caacatattg atctgcaagc cgttgcactt cacctgcgcg tggcgatgcc
    30481 agtcaatgtt gtagaggctg acaaacagac cgcacagacc gagcgtaatc agccaaatcg
    30541 cgttaagcgg agagatttgc acatcgtagc ttaccagcga cagcgcaccg ctcacgccaa
    30601 ccgcgccagt cagtacagtg aagcccgcgg ctgccgtata cagactacca accgcgccgc
    30661 caattccggc tatccagccg cttagcgcct tttgaaaaga aaagagaaat gccagaacag
    30721 cggcggcgac aaaccacgcc acgccgctat tgatcaggga aattgcgctc atttagcctc
    30781 tccactttga gcctgctgaa acaaggtgag atcgccaaag tccgtgttaa aggtcagctc
    30841 acgcttacgt ttgctgacgc gggcgatatc ggtgttatcc accagatgca gggctttagt
    30901 cgggcacatc cgcacgcagg ccggaccttg ttcatcaaag ctacaaaggt cacatttgac
    30961 ggcgatcgcg cgaatacccg gcacccagtc aagcagcgta ctgatacgcg ccggagcagg
    31021 cggtgccggt ggcgctttcg gggtattggc atttgcagga atatccagcg gacggctgcc
    31081 ggaaaattca attgcgccaa acgggcaggc gatgccgcac agcttgcagc ttacgcacag
    31141 gctttcattc aactgcacgg ccccatcaac gcgggtgatg gcgttaaccg ggcagaccac
    31201 cgcgcagggt gcatcttcac agtggtgaca gagctgcggc gcagattctt tttcatttag
    31261 catcactctc aggcgcggca ttgattgcag gccgtgctgg cgatgcgtct ctgaacaggc
    31321 ggcctcacaa gtgtggcaac cgatacagag cgtggagtca gcaattacaa aacgattcac
    31381 caggcattcc tcaggtgatt gtcatttttg acgaaaacat gccgatgaaa tgtcattttc
    31441 gacactcatc gacacgccca tccccaaaca ggcgtaacgc ctgcaaaacg ggcaaagcct
    31501 cagctcatgc tgccgggctt tgtcccttta ccagttggct taaattcacc ggcacattgt
    31561 tttcaatagc ggtgtataag ctgtcgtaaa cgccagcgat tttttcgaga tagtgttcca
    31621 gaacctgttc gcgatcgcgg atgttgacct tgccatcaat catgccgtca atgctcagtt
    31681 gcgcctgcgc aatccgctgt ttgtcttcgc catcttttgt ttcgacgtaa tactcgatgg
    31741 tgtggctgtt aaagccatcc gccagggtga cgtaaatacg aaaatgttca aaaaggacga
    31801 aacagagttc tttgtccggc gcgcagctca tggcgtgatg caggcgcgct ttcgataacg
    31861 tgatcccctg aaccagcgaa ttgcagtaga tgtgccactg gtcctgtagg cgacgatgcc
    31921 gctgtgcgat gtaatcggct ttctcgctta tttcccaaat agtcattgtc aggttacccg
    31981 tttaacagag atgccagctt taagcatttt ttgtgccaac ttttaattta ttattattaa
    32041 agcgattttt aagattttgg tgaatttcgt cgccgtgtcg acgtgtcatt tcgacatcat
    32101 cgacattatt cactgcaggg ataatcaaca ctggcacaat tattgcttgt agctggcaat
    32161 agttaatggg aggcgatatg cacgaaataa ccctctgcca acgggcactg gaattgatcg
    32221 aacagcaggc cgcaaaacac ggcgcaaaac gcgtaactgg ggtctggctc aaaattggcg
    32281 cattttcttg tgtcgaaacc agctctcttg ccttttgttt tgatctggtt tgccgcggca
    32341 gcgtggcgga aggttgtaaa ctgcacctcg aagaacaaga ggccgaatgc tggtgtgaaa
    32401 catgccaaca gtatgtgacg ctactgaccc agcgcgtccg ccgctgtcca cagtgtcatg
    32461 gtgacatgct acagattgtg gcagacgacg gtttacagat tcggcggata gaaatagacc
    32521 aggagtgagc gatgtgtaca acatgcggtt gcggtgaagg caacctgtat atcgagggtg
    32581 atgaacataa ccctcattcc gcgtttcgta gcgcgccatt tgccccggcg gcacgcccga
    32641 agatgaaaat caccggcatt aaagcgcctg aatttacccc cagccagact gaagaaggcg
    32701 acctgcatta cggtcatggc gaagcgggca ctcacgcacc gggcatgagc cagcgtcgga
    32761 tgctggaagt cgaaattgac gtgctggaca aaaataaccg tctggctgaa cgcaaccgcg
    32821 cgcgctttgc tgcctgcaag caactggtgc tcaacctggt ttccagccca ggttccggta
    32881 aaaccaccct gctgacggaa accttaatgc gcctgaaaga cagcgttccg tgcgcggtta
    32941 ttgaaggtga ccagcaaacc gtgaacgatg ccgcacgcat tcgcgctacc ggcacaccag
    33001 cgattcaggt gaacaccggt aaaggctgcc atcttgacgc acagatgatt gccgacgccg
    33061 caccgcgtct gccactggac gataacggta ttctgtttat cgaaaacgtt ggcaacctcg
    33121 tatgcccggc cagcttcgat ctcggtgaaa aacacaaagt ggcggtgctt tccgttaccg
    33181 aaggtgaaga caaaccgctg aaatatccgc atatgtttgc cgccgcctcg ctgatgctgc
    33241 tcaacaaagt tgacctgttg ccgtatctca actttgacgt tgagaagtgc atcgcctgcg
    33301 cccgcgaagt caatccagaa attgaaatca tccttatttc cgccaccagc ggcgaaggga
    33361 tggaccagtg gctgaactgg ctggagacac agcgatgtgc ataggcgttc ccggccagat
    33421 ccgcaccatt gacggtaacc aggcgaaagt cgacgtctgc ggcattcagc gcgatgtcga
    33481 tttaacgtta gtcggcagct gcgatgaaaa cggtcagccg cgcgtgggcc agtgggtact
    33541 ggtacacgtt ggctttgcca tgagcgtaat taatgaagcc gaagcacgcg acactctcga
    33601 cgccttgcaa aacatgtttg acgttgagcc ggatgtcggc gcgctgttgt atggcgagga
    33661 aaaataatgc gttttgttga tgaatatcgc gcgccggaac aggtgatgca gttaattgag
    33721 catctgcgcg aacgtgcttc acatctctct tacaccgccg aacgccctct gcggattatg
    33781 gaagtgtgcg gtggtcatac ccacgccatt tttaaattcg gcctcgacca gttactgcct
    33841 gaaaacgttg agtttatcca cggtccgggt tgcccggtgt gcgtactgcc gatgggcaga
    33901 atcgacacct gcgtggagat tgccagccat ccggaagtca tcttctgtac ctttggcgac
    33961 gccatgcgcg tgccggggaa acagggatcg ctgttgcagg caaaagcacg cggtgccgat
    34021 gtgcgcatcg tctattcgcc gatggatgcg ttgaaactgg cgcaggagaa tccaacccgc
    34081 aaagtggtgt tcttcggctt aggttttgaa accaccatgc cgaccaccgc catcactctg
    34141 caacaggcga aagcgcgtga tgtgcagaat ttttacttct tctgccagca tattacgctt
    34201 atcccgacgc tgcgcagttt gctggaacag ccggataacg gtatcgacgc gttcctcgcg
    34261 ccgggccacg ttagtatggt tatcggcact gatgcctata attttatcgc cagcgatttt
    34321 cagcgtccgc tggtggtggc tggtttcgaa ccccttgatc tactgcaagg cgtggtcatg
    34381 ctggtggagc agaaaatagc ggcccacagc aaggtagaga atcagtatcg tcgggtggta
    34441 ccggatgccg gtaacctgct ggcgcaacag gcgattgccg atgtgttctg tgtcaacggc
    34501 gacagcgaat ggcgcggctt aggcgtgatt gaatcttctg gcgtgcacct gacgccggat
    34561 tatcaacgat tcgatgccga agcacatttc cgcccggcac cgcagcaggt ctgcgatgac
    34621 ccgcgcgcgc gttgtggcga agtcttgacg ggcaaatgta agccgcatca atgcccgctg
    34681 tttggtaaca cctgtaatcc tcaaaccgcg tttggtgcgc tgatggtttc ctccgaagga
    34741 gcgtgcgccg cgtggtatca gtatcgtcag caggagagtg aagcgtgaat aatatccaac
    34801 tcgcccacgg tagcggcggc caggcgatgc agcaattaat caacagcctg tttatggaag
    34861 cctttgccaa cccgtggctg gcagagcagg aagatcaggc acgtcttgat ctggcgcagc
    34921 tggtagcgga aggcgaccgt ctggcgttct ccaccgacag ttacgttatt gacccgctgt
    34981 tcttccctgg cggtaatatc ggcaagctgg cgatttgcgg caccgcgaat gacgttgcgg
    35041 tcagtggcgc tattccgcgc tatctctcct gtggctttat cctcgaagaa ggattgccga
    35101 tggagacact gaaagccgta gtgaccagca tggcagaaac cgcccgcacg gcaggcattg
    35161 ccatcgttac tggcgatact aaagtggtgc agcgcggcgc ggcagataaa ctgtttatca
    35221 acaccgcggg catgggcgca attccgacga atattcactg gggcgcacag acgctaaccg
    35281 caggcgatat attgctggtt agcggtacac tcggcgacca cggggcgact atccttaacc
    35341 tgcgtgagca gctggggctg gatggcgaac tggtcagcga ctgcgcggtg ctgacgccgc
    35401 ttattcagac gctgcgtgac attcccggcg tgaaagcgct gcgtgatgcc acccgtggtg
    35461 gtgtaaacgc ggtggttcat gagttcgcgg cagcctgcgg ttgcggtatt gaaatttctg
    35521 aatcagcgct gccggttaaa cctgccgtgc gcggcgtttg cgaattgctg ggactggacg
    35581 ccctgaactt tgccaacgaa ggcaaactgg tgatcgccgt tgaacgcaac gcggcagagc
    35641 aagtgctggc agcgttacat tcccatccac tggggaaaga cgcggcgctg attggtgaag
    35701 tggtggaacg taaaggtgtt cgtcttgccg gtctgtatgg cgtgaaacga accctcgatt
    35761 taccacacgc cgaaccgctt ccgcgtatat gctaataaaa ttctaaatct cctatagtta
    35821 gtcaatgacc ttttgcaccg ctttgcggtg ctttcctgga agaacaaaat gtcatataca
    35881 ccgatgagtg atctcggaca gcaagggttg ttcgacatca ctcggacact attgcagcag
    35941 cccgatctgg cctcgctgtg tgaggctctt tcgcaactgg taaagcgttc tgcgctcgcc
    36001 gacaacgcgg ctattgtgtt gtggcaagcg cagactcaac gtgcgtctta ttacgcatcg
    36061 cgtgaaaaag acacccccat taaatatgaa gacgaaactg ttctggcaca cggtccggta
    36121 cgcagcattt tgtcgcgccc tgatacgttg cattgcagtt acgaagaatt ttgtgaaacc
    36181 tggccgcagc tggccgcagg tgggctatac ccaaaatttg gtcactattg cctgatgcca
    36241 ctggcggcgg aagggcatat ttttggtggc tgtgaattta ttcgttatga cgatcgcccc
    36301 tggagcgaaa aagagttcaa tcgtctgcaa acatttacgc agatcgtttc tgtcgtcacc
    36361 gaacaaatcc agagtcgcgt cgttaacaat gtcgactatg agttgttatg ccgggaacgc
    36421 gataacttcc gcatcctggt cgccatcacc aacgcggtgc tttcccgcct ggatatggac
    36481 gaactggtca gcgaagtcgc caaagaaatc cattactatt tcgatattga cgatatcagt
    36541 atcgtcttac gcagccaccg taaaaacaaa ctcaacatct actccactca ctatcttgat
    36601 aaacagcatc ccgcccacga acagagcgaa gtcgatgaag ccggaaccct caccgaacgc
    36661 gtgttcaaaa gtaaagagat gctgctgatt aatctccacg agcgggatga tttagccccc
    36721 tatgaacgca tgttgttcga tacctggggc aaccagattc aaaccttgtg cctgttaccg
    36781 ctgatgtctg gcgacaccat gctgggcgtg ctgaaactgg cgcaatgtga agagaaagtg
    36841 tttaccacta ccaatctgaa tttactgcgc cagattgccg aacgtgtggc aatcgctgtc
    36901 gataacgccc tcgcctatca ggaaatccat cgtctgaaag aacggctggt tgatgaaaac
    36961 ctcgccctga ccgagcagct caacaatgtt gatagtgaat ttggcgagat tattggccgc
    37021 agcgaagcca tgtacagcgt gcttaaacaa gttgaaatgg tggcgcaaag tgacagtacc
    37081 gtgctgatcc tcggtgaaac tggcacgggt aaagagctga ttgcccgtgc tatccataat
    37141 ctcagtgggc gtaataatcg ccgcatggtc aaaatgaact gcgcggcgat gcctgccgga
    37201 ttgctggaga gcgatctgtt tggtcatgag cgtggggctt ttaccggtgc cagcgcccag
    37261 cgtatcggtc gttttgaact ggcggataaa agctccctgt tcctcgacga agtgggcgat
    37321 atgccactgg agttacagcc gaagttgctg cgtgtattgc aggaacagga gtttgaacgc
    37381 ctcggcagca acaaaatcat tcagacggac gtgcgtctaa tcgccgcgac taaccgcgat
    37441 ctgaaaaaaa tggtcgccga ccgtgagttc cgtagcgatc tctattaccg cctgaacgta
    37501 ttcccgattc acctgccgcc actacgcgag cgtccggaag atattccgct gctggcgaaa
    37561 gcctttacct tcaaaattgc ccgtcgtctg gggcgcaata tcgacagcat tcctgccgag
    37621 acgttgcgca ccttgagcaa tatggagtgg ccgggtaacg tacgcgaact ggaaaacgtc
    37681 attgagcgcg cggtattgct aacacgcggc aacgtgctgc agctgtcatt gccagatatt
    37741 gctttaccgg agcctgaaac gccgcctgcc gcaacggttg tcgctcagga gggcgaagat
    37801 gaatatcagt tgattgttcg cgtgctgaaa gaaactaacg gcgtggttgc cgggcctaaa
    37861 ggcgctgcgc aacgtctggg gctgaaacgc acgaccctgc tgtcacggat gaagcgactg
    37921 ggaattgata aatcggcatt gatttaactg caaattgccg gacagatctg cctgtccggc
    37981 atactattca cgaggttttt tcggacgata tttttccggc agttctggca ccggacactt
    38041 gtcatcgatg agatgacgca cggttaagat cggatgacgc cacagcattc tcggcccggc
    38101 ccaacgcata atctgtttca tctcttcacg ctttgcaggc tggtaacagt gcaccggaca
    38161 ctgcttacag gctggtttct cttcgccgaa cacacattta tccagccgct tttgcgcgta
    38221 aacaaacaac gcctcgtaat gctccggctc cgctgacgcc tgcgggcatt tcgcttgata
    38281 aagatcgatc atttttttaa tcgtcagttt ttcacgagag atacgcttgc cggacatact
    38341 gcctccacct cattaagatg catttatatt acaacttaat cttaaagggc actatgactc
    38401 taaagaagaa gggttagcca accgatacaa ttttgcgtac ttgcttcata agcatcacgc
    38461 aaaagctgca aaacagcatc tttcccggaa ccagcatcaa gaactcgccg ttcgcttctt
    38521 cccctgaaat gattaactcc ggtatcatgt gcgccttatg tgattacaac gaaaataaaa
    38581 accatcacac ctcatttaat atcagggaac cggacataac cccatgagtg caatagaaaa
    38641 tttcgacgcc catacgccca tgatgcagca gtatctcaag ctgaaagccc agcatcccga
    38701 gatcctgctg ttttaccgga tgggtgattt ttatgaactg ttttatgacg acgcaaaacg
    38761 cgcgtcgcaa ctgctggata tttcactgac caaacgcggt gcttcggcgg gagagccgat
    38821 cccgatggcg gggattccct accatgcggt ggaaaactac ctcgccaaac tggtgaatca
    38881 gggcgagtcc gttgccatct gcgaacaaat tggcgatccg gcgaccagca aaggtccggt
    38941 tgagcgcaaa gttgtgcgta tcgttacgcc aggcaccatc agcgatgaag ccctgttgca
    39001 ggagcgtcag gacaacctgc tggcggctat ctggcaggac agcaaaggtt tcggctacgc
    39061 gacgctggat atcagttccg gtcgttttcg cctgagcgaa ccggctgacc gggaaacgat
    39121 ggcggcagaa ctgcaacgca ctaatcctgc ggaactgctg tatgcagaag attttgctga
    39181 aatgtcgtta attgaaggcc gtcgcggcct gcgccgtcgc ccgctgtggg agtttgaaat
    39241 cgacaccgcg cgccagcagt tgaatctgca atttgggacc cgcgatctgg tcggttttgg
    39301 cgtcgagaac gcgccgcgcg gactttgtgc tgccggttgt ctgttgcagt atgcgaaaga
    39361 tacccaacgt acgactctgc cgcatattcg ttccatcacc atggaacgtg agcaggacag
    39421 catcattatg gatgccgcga cgcgtcgtaa tctggaaatc acccagaacc tggcgggtgg
    39481 tgcggaaaat acgctggctt ctgtgctcga ctgcaccgtc acgccgatgg gcagccgtat
    39541 gctgaaacgc tggctgcata tgccagtgcg cgatacccgc gtgttgcttg agcgccagca
    39601 aactattggc gcattgcagg atttcaccgc cgagttgcag ccggtactac gtcaggtcgg
    39661 cgacctggaa cgtattctgg cgcgtctggc gttgcgtacc gctcgcccac gcgatctggc
    39721 ccgtatgcgt cacgctttcc agcaactgcc ggagctgcgt gcgcagttag aaactgttga
    39781 tagtgcacca gtacaggcgc tacgtgagaa gatgggcgag tttgccgagc tgcgcgatct
    39841 gctggagcga gcaatcatcg acacaccgcc ggtgctggta cgcgacggtg gtgttatcgc
    39901 atcaggctat aacgaagagc tggatgagtg gcgcgcgctg gctgacggcg cgaccgatta
    39961 tctggagcgt ctggaagtcc gcgagcgtga acgtaccggc ctggacacgc taaaagttgg
    40021 ctttaatgcg gtgcacggct actacattca aatcagccgt gggcaaagcc atctggcacc
    40081 tatcaactat atgcgtcgcc agacgctgaa aaacgccgag cgctacatca ttccagagct
    40141 aaaagagtac gaagataaag tcctcacttc aaaaggcaaa gcactggctc tggaaaaaca
    40201 gctttatgaa gagctgttcg acctgctgtt gccgcatctg gaagcgttgc aacagagcgc
    40261 gagcgcgctg gcggaactcg acgtgctggt gaacctggcg gaacgggcct ataccctgaa
    40321 ctacacctgc ccgaccttca ttgataaacc gggcattcgc attaccgaag gccgccatcc
    40381 ggtggttgaa caggtgctga acgagccatt tatcgccaac ccgctgaatc tgtcaccgca
    40441 gcgccggatg ttgattatta ccggtccgaa catgggcggt aaaagtacct atatgcgcca
    40501 gaccgcactg attgcgctga tggcctatat cggcagctac gtaccggcgc aaaaagtcga
    40561 gattggcccg attgaccgta tctttacccg cgtaggggca gcggatgatc tggcttccgg
    40621 gcgttcaacc tttatggtgg agatgaccga aaccgctaat attctgcata acgccaccga
    40681 gtacagtctg gtgctgatgg atgagattgg gcgcggaacg tccacttacg atggtctgtc
    40741 gctggcgtgg gcgtgcgcgg aaaatctggc gaataagatt aaggcgttga cgctgtttgc
    40801 cacccactat ttcgagctga cccggttacc ggagaaaatg gaaggcgtcg ccaacgtgca
    40861 tctcgatgca ctggagcacg gcgacaccat tgcctttatg catagcgtgc aggatggcgc
    40921 ggcgagcaaa agctacggcc tggcggttgc agctctggcc ggcgtgccaa aagaggttat
    40981 taagcgcgca cggcaaaaac tgcgtgagct ggaaagcatt tcgccgaacg ccgccgctac
    41041 gcaagtggat ggtacgcaaa tgtctttgct gtccgtaccg gaagaaacct cgcctgcagt
    41101 cgaggcactg gaaaacctcg atccggattc actcaccccg cgtcaggcgc tggaatggat
    41161 ttatcgcctg aagagtctgg tgtaataata attcccgata gtcttttgct atcgggaata
    41221 ttaacgataa ctgacgaatc aaataaaaat accctgtata ataggaaagc ttattttaca
    41281 gggtaaaacc atgccatcta cacgctatca aaaaatcaat acccatcact atcgccatat
    41341 atgggtcgtt ggtgatattc atggtgaata tcagttatta caatcccgct tacatcaact
    41401 ctcttttttc cccgaaaccg acttacttat ttctgtcggc gataatattg atcgtgggcc
    41461 ggagagtctt aacgtcctgc gcctgctaaa ccagccctgg tttatctccg ttaaaggcaa
    41521 ccacgaagca atggcgctgg atgcgttcgc gaccggcgat ggcaatatgt ggcttgccag
    41581 cggtggtgac tggtttttcg atttaaatga ttcagagcaa aaagaagcta cagatctgtt
    41641 gctgaaattc catcaccttc cacatattat tgaaatcact aacgacaaca taaaatatgt
    41701 catcgcacat gcagattatc cggggagtga atatctcttt ggtaaagaaa tagcggagag
    41761 cgaattactc tggcctgttg atcgtgtgca gaaatcgctt aatggcgagt tacaacaaat
    41821 aaacggcgct gattatttta tat
//
LOCUS       DAZKFZ010000017        40872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000017 DAZKFZ010000000
VERSION     DAZKFZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..750
                     /gene="espX2"
                     /locus_tag="ACHOQW_000864"
     CDS             <1..750
                     /gene="espX2"
                     /locus_tag="ACHOQW_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFP9571984.1"
                     /translation="RLSHQALISMLEPEVKKKTIWNNYSIYPSLQDTHEVVRDDPETI
                     CMRAFPLFAKGWEYAQKNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVLECNNQPTE
                     LNSSFSSLMNDSDQWQQHTLKDKHYANLLTMLDLNDASESDKSKIFFCLSAVFANISH
                     SNVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEANQ
                     LRISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            1034..3184
                     /gene="dinG"
                     /locus_tag="ACHOQW_000865"
     CDS             1034..3184
                     /gene="dinG"
                     /locus_tag="ACHOQW_000865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP9571985.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3212..4174
                     /gene="ybiB"
                     /locus_tag="ACHOQW_000866"
     CDS             3212..4174
                     /gene="ybiB"
                     /locus_tag="ACHOQW_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP9571986.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4315..5400
                     /gene="hcxB"
                     /locus_tag="ACHOQW_000867"
     CDS             4315..5400
                     /gene="hcxB"
                     /locus_tag="ACHOQW_000867"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP9571987.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5467..5526
                     /gene="ybiE"
                     /locus_tag="ACHOQW_000868"
     CDS             5467..5526
                     /gene="ybiE"
                     /locus_tag="ACHOQW_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP9571988.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5629..5889)
                     /gene="ybiJ"
                     /locus_tag="ACHOQW_000869"
     CDS             complement(5629..5889)
                     /gene="ybiJ"
                     /locus_tag="ACHOQW_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP9571989.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6154..6420)
                     /gene="ybiI"
                     /locus_tag="ACHOQW_000870"
     CDS             complement(6154..6420)
                     /gene="ybiI"
                     /locus_tag="ACHOQW_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP9571990.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
     gene            complement(6494..7180)
                     /gene="ybiX"
                     /locus_tag="ACHOQW_000871"
     CDS             complement(6494..7180)
                     /gene="ybiX"
                     /locus_tag="ACHOQW_000871"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP9571991.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7222..9504)
                     /gene="fiu"
                     /locus_tag="ACHOQW_000872"
     CDS             complement(7222..9504)
                     /gene="fiu"
                     /locus_tag="ACHOQW_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP9571992.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9769..10029)
                     /gene="mcbA"
                     /locus_tag="ACHOQW_000873"
     CDS             complement(9769..10029)
                     /gene="mcbA"
                     /locus_tag="ACHOQW_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP9571993.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10305..11231
                     /gene="rlmF"
                     /locus_tag="ACHOQW_000874"
     CDS             10305..11231
                     /gene="rlmF"
                     /locus_tag="ACHOQW_000874"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP9571994.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11228..13453)
                     /gene="ybiO"
                     /locus_tag="ACHOQW_000875"
     CDS             complement(11228..13453)
                     /gene="ybiO"
                     /locus_tag="ACHOQW_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP9571995.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13570..14292)
                     /gene="glnQ"
                     /locus_tag="ACHOQW_000876"
     CDS             complement(13570..14292)
                     /gene="glnQ"
                     /locus_tag="ACHOQW_000876"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP9571996.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14289..14948)
                     /gene="glnP"
                     /locus_tag="ACHOQW_000877"
     CDS             complement(14289..14948)
                     /gene="glnP"
                     /locus_tag="ACHOQW_000877"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP9571997.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15086..15832)
                     /gene="glnH"
                     /locus_tag="ACHOQW_000878"
     CDS             complement(15086..15832)
                     /gene="glnH"
                     /locus_tag="ACHOQW_000878"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP9571998.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16236..16739)
                     /gene="dps"
                     /locus_tag="ACHOQW_000879"
                     /gene_synonym="pexB"
     CDS             complement(16236..16739)
                     /gene="dps"
                     /locus_tag="ACHOQW_000879"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP9571999.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(17038..17925)
                     /gene="rhtA"
                     /locus_tag="ACHOQW_000880"
     CDS             complement(17038..17925)
                     /gene="rhtA"
                     /locus_tag="ACHOQW_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP9572000.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            18160..18225
                     /locus_tag="ACHOQW_000881"
     CDS             18160..18225
                     /locus_tag="ACHOQW_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572001.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            18278..18793
                     /gene="ompX"
                     /locus_tag="ACHOQW_000882"
     CDS             18278..18793
                     /gene="ompX"
                     /locus_tag="ACHOQW_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP9572002.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18842..20425)
                     /gene="opgE"
                     /locus_tag="ACHOQW_000883"
     CDS             complement(18842..20425)
                     /gene="opgE"
                     /locus_tag="ACHOQW_000883"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9572003.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20697..20825)
                     /gene="mntS"
                     /locus_tag="ACHOQW_000884"
     CDS             complement(20697..20825)
                     /gene="mntS"
                     /locus_tag="ACHOQW_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP9572004.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21011..21478
                     /gene="mntR"
                     /locus_tag="ACHOQW_000885"
     CDS             21011..21478
                     /gene="mntR"
                     /locus_tag="ACHOQW_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP9572005.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21475..22593
                     /gene="ybiR"
                     /locus_tag="ACHOQW_000886"
     CDS             21475..22593
                     /gene="ybiR"
                     /locus_tag="ACHOQW_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP9572006.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(22651..23571)
                     /gene="ldtB"
                     /locus_tag="ACHOQW_000887"
     CDS             complement(22651..23571)
                     /gene="ldtB"
                     /locus_tag="ACHOQW_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9572007.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23790..25382
                     /gene="ybiT"
                     /locus_tag="ACHOQW_000888"
     CDS             23790..25382
                     /gene="ybiT"
                     /locus_tag="ACHOQW_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP9572008.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25550..26815)
                     /gene="ybiU"
                     /locus_tag="ACHOQW_000889"
     CDS             complement(25550..26815)
                     /gene="ybiU"
                     /locus_tag="ACHOQW_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP9572009.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(26967..27782)
                     /gene="ybiV"
                     /locus_tag="ACHOQW_000890"
     CDS             complement(26967..27782)
                     /gene="ybiV"
                     /locus_tag="ACHOQW_000890"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP9572010.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(27928..30359)
                     /gene="ybiW"
                     /locus_tag="ACHOQW_000891"
                     /pseudo
     CDS             complement(27928..30359)
                     /gene="ybiW"
                     /locus_tag="ACHOQW_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            complement(30365..31264)
                     /gene="ybiY"
                     /locus_tag="ACHOQW_000892"
     CDS             complement(30365..31264)
                     /gene="ybiY"
                     /locus_tag="ACHOQW_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP9572011.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            31395..32057
                     /gene="fsa"
                     /locus_tag="ACHOQW_000893"
     CDS             31395..32057
                     /gene="fsa"
                     /locus_tag="ACHOQW_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9572012.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(32236..32985)
                     /gene="moeB"
                     /locus_tag="ACHOQW_000894"
     CDS             complement(32236..32985)
                     /gene="moeB"
                     /locus_tag="ACHOQW_000894"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP9572013.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(32985..34220)
                     /gene="moeA"
                     /locus_tag="ACHOQW_000895"
     CDS             complement(32985..34220)
                     /gene="moeA"
                     /locus_tag="ACHOQW_000895"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP9572014.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            34424..35389
                     /gene="iaaA"
                     /locus_tag="ACHOQW_000896"
                     /pseudo
     CDS             34424..35389
                     /gene="iaaA"
                     /locus_tag="ACHOQW_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            35376..37247
                     /gene="gsiA"
                     /locus_tag="ACHOQW_000897"
     CDS             35376..37247
                     /gene="gsiA"
                     /locus_tag="ACHOQW_000897"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP9572015.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            37267..38805
                     /gene="gsiB"
                     /locus_tag="ACHOQW_000898"
     CDS             37267..38805
                     /gene="gsiB"
                     /locus_tag="ACHOQW_000898"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP9572016.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            38823..39743
                     /gene="gsiC"
                     /locus_tag="ACHOQW_000899"
     CDS             38823..39743
                     /gene="gsiC"
                     /locus_tag="ACHOQW_000899"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP9572017.1"
                     /translation="MLNYVIKRLLGLIPTLFIVLVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            39746..40657
                     /gene="gsiD"
                     /locus_tag="ACHOQW_000900"
     CDS             39746..40657
                     /gene="gsiD"
                     /locus_tag="ACHOQW_000900"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP9572018.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         9743 a        10148 c        11022 g         9959 t
ORIGIN      
        1 cgcttatcgc accaggcgtt aatctcaatg cttgagccag aggttaagaa aaagactatc
       61 tggaataatt actctattta tccatctttg caggataccc atgaagttgt cagagatgat
      121 ccagaaacga tttgtatgcg tgcatttcct ttatttgcaa aaggatggga atatgcgcag
      181 aaaaataaga aacatcaatt gatcctcaat gctcttggat tcaaaggtta tattcgtgat
      241 atatttatgt cagcaataat gcgaaaaaca gattttgtgc tcgaatgcaa taatcaaccg
      301 acagaactta attcatcatt ttcctctttg atgaacgatt cagatcaatg gcaacagcat
      361 actctgaaag ataaacatta cgctaatcta ttaacgatgc tggatctcaa tgatgccagt
      421 gaaagtgata agtctaaaat atttttctgc ctgtctgccg tatttgcaaa tatttctcac
      481 agtaatgtct ttaacggtat tcctgatgcg tcaaaaactt taaaacgtta tgcatttgct
      541 ttattggcga aagctcattc tttagatgaa agtatgataa gcaatcaaac ttttaataca
      601 tacaaaacgg tattgcttga tttcaataat ctgtctaatg aagaggccaa ccagttgcgc
      661 ataagttcgt tatatcgtga tatggtccgc tatgcacaat atcgtttttc taaagtttta
      721 tccgagtgga cgccagacgc atggctgtaa agatcgcagc agaataatgg cattgatggc
      781 cctggctcag gccagggtcg gcaataccgg ttcctgcgaa gcgatgacgg cggcaattgt
      841 aaccacgaaa tgcggtatca cttgctgttt tctgccctga agactggcgc tcactgtgcc
      901 gtcgaatttt caatcaaata tattgcgaga gggcgtgttt gcgagccgct ttccagaaac
      961 agaaaaacca ttacccctga aaaccgaaaa atgccacaat attggctgtt tatacagtat
     1021 ttcaggtttt ctcatggcat taaccgccgc gcttaaagcg caaattgccg cctggtataa
     1081 ggcgcttcag gaacagatcc ccgactttat tccccgtgcg ccgcagcggc agatgattgc
     1141 ggacgtcgcc aaaacgctgg ccggagaaga agggcggcat ctggcgattg aagcccccac
     1201 tggcgttggg aaaacgctct cctatttgat tcccggcatc gccattgccc gcgaagagca
     1261 aaaaacgctg gtggtgagta ccgccaacgt ggcattgcag gatcagatct acagcaaaga
     1321 tttgccactg ctgaaaaaga tcattcccga tcttaaattc actgccgctt ttgggcgtgg
     1381 gcgctacgtt tgcccgcgta atctgacggc gcttgccagc actgagccca cgcaacagga
     1441 tctgctggcg tttcttgacg acgaactgac gccaaataat caggaagagc aaaaacgttg
     1501 tgcgaagctg aagggcgatc tcgacactta taaatgggat ggtctgcgcg atcatacgga
     1561 tatcgctata gatgacgatc tctggcgtcg tttaagtacc gacaaagcca gctgcctcaa
     1621 ccgcaactgt tactactatc gcgaatgccc gttttttgtc gctcgtcggg aaattcagga
     1681 agcggaagtg gtggtggcaa accatgcgct ggtgatggcg gcgatggaaa gcgaagccgt
     1741 attgcctgac ccgaaaaatt tactgctggt gctggacgaa ggccatcacc tgccagatgt
     1801 ggcgcgggat gcgctggaga tgagtgccga aatcaccgcg ccatggtatc ggctacagct
     1861 ggacttgttc acgaaactgg tcgctacctg catggagcag tttcgcccga agaccatccc
     1921 gccgttggcg atccctgaac gtttgaatgc gcattgtgaa gagctgtatg agcttatcgc
     1981 ctcattaaac aacattctca atctctacat gcctgccggg caggaagcag aacaccgttt
     2041 tgcgatgggc gaactgcctg atgaagtgct ggagatctgc cagcggctgg caaaactcac
     2101 cgagatgctg cgtggtctgg cggagttatt tcttaacgat ttaagtgaga aaaccggcag
     2161 ccatgacatt gtacgtctgc atcggttgat tttgcagatg aaccgcgcgt tggggatgtt
     2221 cgaggcgcaa agcaaactct ggcggctggc ttcgctggcg caatcttccg gtgcaccggt
     2281 gaccaaatgg gcgacgcggg aagagcgcga agggcagcta cacctctggt ttcactgcgt
     2341 gggaatacgt gtcagcgatc agctggaaag gctgctgtgg cgcagtattc cgcacattat
     2401 tgtcacctcc gcaaccttgc gttcgctgaa cagtttttcg cgtttgcagg agatgagcgg
     2461 gctgaaagag aaagcgggcg accgttttgt ggcgctggat tcccccttta accactgcga
     2521 acagggcaaa attgttattc cccggatgcg cgttgagcct tccatcgaca acgaagaaca
     2581 gcatattgct gaaatggcgg cctttttccg tgagcaggtg gagagcaaaa aacatctcgg
     2641 tatgttggtg ctgtttgcca gcgggcgtgc gatgcagcgc tttctcgact atgtgacgga
     2701 tttacgtctg atgttgctgg tgcagggcga tcagccgcgt taccgtttag ttgaactgca
     2761 ccgcaaacgc gtcgccaacg gtgagcgtag tgtgctggtg ggcttacagt catttgccga
     2821 agggcttgat ttgaaaggtg atatgctcag ccaggtgcat atccacaaaa tcgcttttcc
     2881 gcctatcgac agcccggtgg tgatcactga aggggaatgg ctgaaaagcc tcaaccgcta
     2941 tccatttgag gtgcaaagcc tgccgagcgc ctcgtttaac ctgattcagc aggttgggcg
     3001 actgattcga agtcacggtt gctggggcga agtggtgatt tacgataaac gcttgctgac
     3061 caaaaattat ggcaagcgac tactggatgc attaccggta tttccgatag agcaaccgga
     3121 agtccctgaa ggtatagtta aaaagaaaga aaaaacgaaa tccccacgcc gtcggcggcg
     3181 ttaatgatgt gagtcaggta aggagtcgta aatggactat cgcaaaatca ttaaagagat
     3241 cgggcgcggg aaaaaccacg cgcgcgattt agaccgggat actgcgcgcg gtctgtatgc
     3301 ccatatgctc aatggtgaag tccctgacct tgagttgggc ggtgtattga ttgcgctgcg
     3361 tatcaaaggg gaaggggaag cagagatgct cggcttttac gaagccatgc aaaatcacac
     3421 catcaagctg acgccgcctg caggcaagcc gatgccgatt gtcatcccca gttacaacgg
     3481 cgcgcgtaaa caggccaacc tgacgccgtt gttggcgatt ctcctgcaca aactcggttt
     3541 tcctgtggtg gttcacgggg ttagcgaaga tccaactcgc gtgctgaccg aaaccatttt
     3601 cgaactgatg ggtattacgc caacgttgca cggcgggcag gcgcaggcga agctcgacga
     3661 acatcaaccg gtgtttatgc ctgtcggcgc gttttgcccg ccgctggaaa aacaactggc
     3721 gatgcgctgg cggatgggcg tgcgtaacag tgcacatacc ctggcgaaac tggcgacgcc
     3781 atttgccgaa ggtgaggcac tgcgtctttc cagcgtttcg catccggaat acatcgggcg
     3841 agtcgcgaag ttctttagcg atatcggcgg gcgggcgtta ctaatgcatg gtactgaagg
     3901 cgaagtgtat gctaatccgc agcgctgccc acagatcaat ctcattgatc gtgaagagat
     3961 gcgggtgctg tatgaaaaac aggaccctgc tggaagcgag ttactgccac aagcaaaaga
     4021 tccggaaacc acggcgcagt ggattcagcg ttgccttgct ggccgcgaac cgattcccga
     4081 atcgctgaaa atccagatgg cttgctgcct ggtggctacg ggtgaagcgg caactatcag
     4141 cgatggcctg gcgcgcgtta atcaggcatt ttaattcttt tctcagccgg atgagccatg
     4201 tcatccggct tttaccccac cgcaatatga aattcctgca tctttattga ccttcccgcg
     4261 cccggcgtgc agcataaaaa aacaacaaac acataacata aacaggagtt aaccatggaa
     4321 agtggtcatc gctttgatgc tcagacgctg cacagcttta ttcaggctgt atttcgtcag
     4381 atgggtagcg aggaacaaga agcgaaatta gttgccgatc atttaatcgc ggcaaacctg
     4441 gcagggcatg attcacatgg tattggcatg atcccaagct atgtacgctc ctggagtcag
     4501 gggcacctgc aaattaacca tcatgccaaa atcgttaaag aggcgggggc ggcggtcacg
     4561 ctcgatggcg atcgcgcatt tggtcaggtc gcggcacatg aagcgatggc gctggggatt
     4621 gagaaagcgc atcagcacgg tattgctgcc gtggcgctac ataactcgca tcatatcggc
     4681 cgtatcggtt actgggcgga gcagtgtgca gcggcggggt ttgtctctat ccactttgtt
     4741 agcgtggtcg gtattccaat ggtcgcaccg ttccacggtc gcgacagccg ctttggcacg
     4801 aatccgttct gtgtggtttt cccacgtaaa gatgattttc cgctgttgct tgattacgcc
     4861 accagcgcca ttgcatttgg caaaacccgc gtcgcctggc ataaaggcgt ccccgtgccg
     4921 ccaggttgcc tgattgacgt taacggcgtg ccgacgacca atccggcggt aatgcaggag
     4981 tcgccgttgg gttcgctgtt gacctttgcc gaacataaag gctacgccct ggcggcaatg
     5041 tgtgaaattc ttggcggggc gctttctggc ggtaaaacga cgcatcagga aacgttacaa
     5101 accagtcccg atgccattct taactgcatg accactatca tcatcaaccc ggaactgttc
     5161 ggcgcgccgg attgtagcgc gcagaccgaa gcctttgccg agtgggtgaa agcctcgccg
     5221 catgatgacg ataagccgat tttgctcccg ggcgagtggg aagtgaacac gcgtcgcgaa
     5281 cggcaggagc aggggattcc attggatgcg ggaagctggc aggccatttg tgatgcggcg
     5341 cggcagattg gtatgccgga agagacgttg caggctttct gtcagcagtt agccagctaa
     5401 aaaaaagccc gtccagtggc ggacgggcaa acaagggtaa cataggatca atgagggtta
     5461 gagcatatgc gtctgtcggc aaacagacag ggaaatactt gtgcaggacg tagcgtaaac
     5521 gcctgatccg tccaacggtt cggattttgt aggcctgata agacgcggta gcgtcgcatc
     5581 aggcatctgt tgccggatgc ggcgtaaacg ccttatccgt cctacgaatt acttataaat
     5641 taccgcagta ccgcttaatt tattgttgtt ggtggcggaa gtgatgctat atccactggc
     5701 accggctgcc gccgctttct cagccagttt cgcttccagg gcatcgaggg tggatgcgcc
     5761 atcggcagaa accacgccga ttttattcat gttctgtgcc tgggatgccg ttaccggttc
     5821 cgcggcaaac acgccaaatg acagagctga aagagccata gcagcaacaa cagtattgat
     5881 agttttcata attaatctct cgcaggtgat ttttgtataa gtgacgttgt ttcgtcgatg
     5941 tgatgagtat cacgtttttt tacgagagat aaaatcgaag agaattgacc gccttgttca
     6001 aataaattga ttgatatcta acatactgat aaaccattta tttgttgtac ttatggactt
     6061 gttttatgca gcgtggcagt cacgacagcg ttaaaggcac ggaatgccac gcaaaataca
     6121 aattatactc gctatacatc tcgccagttg tagttaacgt aactggcttt ctttcgaacc
     6181 tctgcgatta tatcctgtat aagctggttt ttgtaaatct ttctcctgct gacaatgaat
     6241 gcataagcgc acgccaggaa tggcttcccg acgggcttgc gggatggggg caccgcactc
     6301 ttcacattca tacaggcttt cgccacgcgg aatttcaccc cgagcgcggg caatcgcatc
     6361 ttcaattgta ctgttgatct gttcgttgac ggcgtcatcg ttagcccaac cggatgccat
     6421 atcgtcctcc ccatatcaat gcttgtatag ttaagtgtag ctaatatagg gacgaacctg
     6481 ggcagttcaa gcgtcagatc tccgaccatt cccgcagcag attatgataa agattgagca
     6541 gcgacaggat ctcttcattt tcaccatagc ggcttttcag cgactgaata ttctgaatat
     6601 ttttgtccag ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc
     6661 atataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg
     6721 agggatacaa caccagatca cctgccggga gttttacccg atgttgtccg aaggtgtcat
     6781 taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa
     6841 ggtcagtgcg catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatgaaaac
     6901 cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg
     6961 ccgcggcaaa gaacaaagca tgttggttaa ccgcgttcag cacctcattt tgcagggcgg
     7021 cgtataacgt gctgcgggtg tctacctgtt gattgttctt aacttgcgca ccttgtgcgc
     7081 cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaacgggcga
     7141 cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg
     7201 ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg
     7261 ctcgcccggg tgataacggt agccgctctt attgattgag gcgacgtaat cggtatcaaa
     7321 caggttgtag acgtttagct ggaagtcgag attgcgatta accctatacc ccagtttggc
     7381 atcggcgacc cagtaacctt cggtaaacgc tggcgttccc accgcgccgt ctgaaccttt
     7441 atgcatactg ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata
     7501 ttggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg
     7561 ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg
     7621 ccacgcggga gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc
     7681 gtattgcgag taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa
     7741 cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct
     7801 ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccgc tgccagactg
     7861 cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac
     7921 attgccgttt tccgtcaggt gatacagcgc cccggctttc cagttcacca gattgcccga
     7981 cttggcggtg tcgacggtgg tgaccggaga accttttgcc acaccagctg ggcaggtgat
     8041 ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc
     8101 cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaatgcgta
     8161 aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg
     8221 atgaatgctg ctgtcaggat gataaatatt taccgcaggt aacgtcaccg gattaacgcc
     8281 gtagttagtc tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga
     8341 agcggtatag aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc
     8401 tttggtattc gccgtgcgtg accacgtcca gctattcaca tcgctggtgg gctgggtaat
     8461 attcgacgcc ccacccataa tcgccgtcat caggtaatcc tgcttcacgc gcgaccaacg
     8521 ggtagtgttg cgaatggtgg tgttatcgtt gatatcgtgc tcaaaacgca tggtggcggt
     8581 gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgcg tatcaacttt
     8641 tccggaatga ttcagggtcg ccgttcctgc agatggggca gaatagcccg gcaaaccgat
     8701 ggttggaatg ccgccgtctg gcgtgttgtg ctgggtgaca tgcagataat taagatacaa
     8761 acgattcgct gtaccaaggc caaaagcgat agaaggggcg acgccgtaac gctcattttt
     8821 gactttgtcg cgtccggcat cgtgcgtttt ttcgcccatc acattcaggc gcaccgcagt
     8881 ggtatcacca atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat
     8941 actggcggag gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga
     9001 gcccgtcggc gcgctgcgcc cgtagtcggt gccggaaggc cctttaatca cttcgacctg
     9061 ctcagtattg aaggtgtcgc gcgagacgct gccgatatcg cgaatgccat caatataaat
     9121 actgttagag gtatcagcac cgcgcatata aatggcgtcg ccagtggtgg agttaccgtt
     9181 ctcacccgca aaaaacgcac ccacgccggg gacgtttttg agcgcatcgg taaggttggt
     9241 tgcgccctga tctttaatca cttgttcaga aatcaccgtc atcgtgcggg tagtatccgc
     9301 taccggacgc gagaatttcg gatcggcaga ttgttgtggc gcataaagcg aaggcgttga
     9361 tgcttcgaca accagcgtgt catccgcgtt agtttgccct tcggcggcga gtgcctgagc
     9421 cacaggcgtg atgccaatac aaagaccggc aaagaacgtg agcgaatgaa attgtctggc
     9481 agggaaattg cgattgtttt ccattttgca ggtgactttt tcttatatgt tgaaattttc
     9541 actgccacga gaaagcctct ctgcggatgc gatgccactg ttaatctcgt gatagtgttc
     9601 gtgcaagtga taataatttt gataagcatt atcacttggc gcgattttct atcacccatt
     9661 gcgcgaaaaa gaaatacatt tatttacatt gcgttcatat ttgatgtttt ttgattacaa
     9721 aacaggcgaa aactgcatcc agaggcgaat gcagttacgt tggtggggtt atttgtagat
     9781 ggttgcagta ccgaacatct ggtcatttcc gcctgcggaa ttaatgacat aacctttcgc
     9841 gccttgctcg cgcgcttttt ctgccagttt atcctccaga tcgcttaggt ttgatgcgcc
     9901 agttgccgaa acggtaccgg cgggccgtag ctgaccgctg tccaggttac tcatcggttg
     9961 cgcggcataa gccgtgagag agattccgct caggacggtg gcaatcagta gtgtgaggca
    10021 ctttttcata atgacatcct cataaataca acgggtagtg cctgataagt gtaggtcgcc
    10081 tgcgcgatgc aaatgacgca aaaaattgac agagatgtgc ttattttttg aacgatatat
    10141 ttttacaaaa taagacaaat caatgacatg cataatgctg atttttatga tgaattgcca
    10201 taaaccatag ccatgttaag gttttgggga atgataaacg cagccagata tttaccctgt
    10261 cgcgttatgc gggtatctta cgccgctgct taaaggagaa tgctatgtcc gcccagaaac
    10321 cggggttgca tccgcgcaac cgtcatcaca gccgctacga tctcgccacg ctttgtcagg
    10381 tcaatcctga actcaagcaa ttcctcacgc ttacacccgc cggggagcaa agcgtagact
    10441 ttgccaatcc gctggcggtg aaggcgctca ataaggcgtt gctggcccat ttttacgccg
    10501 tagcgaactg ggatatcccg gacggttttc tctgcccgcc agtaccgggc cgggcggatt
    10561 atattcatca ccttgccgat ttactggctg aagcaagcgg aacaattcca gctaatgcca
    10621 gcattcttga tatcggcgtt ggtgcgaact gtatttatcc gctgattggc gtacatgaat
    10681 atggctggcg ttttaccggt agtgaaacca gcagccaggc gttaagcagc gcgcaggcga
    10741 tcatcagtgc taatccgggg cttaatcgcg ccattcgtct gcgtcggcaa aaagagagtg
    10801 gggcgatttt taacggcatc atccataaaa acgagcaata cgacgcgacc ttgtgtaacc
    10861 cgccattcca cgattccgcc gctgcggcgc gggcaggtag tgagcgtaaa cgccgtaacc
    10921 tggggctgaa caaagacgat gcactgaact ttggcggcca gcaacaggag ttgtggtgtg
    10981 aaggcggtga ggtcaccttt atcaaaaaga tgattgaaga gagcaaaggc ttcgcgaagc
    11041 aggtgatgtg gtttacttca ctggtatctc gtggtgaaaa cttaccgccg ttgtatcgtg
    11101 ccctgacgga cgtgggcgcg gtgaaggtgg ttaaaaaaga gatggcccag gggcaaaagc
    11161 agagtcgctt tattgcctgg acctttatga acgacgagca gcgccgccgt tttgtcaatc
    11221 gccagcgtta aagcgttggc tcccccggcg gtaacggttc agccggggtc gcgcccggag
    11281 caggcagcac ctgataagtc tgcaccggcg cgcgaacgcc cgccaggtcg aaatgttttt
    11341 tcacctggct gtcgagggca aagcgtaccg tccactgttt gagtggcagc gtggtgaacg
    11401 aaacacgcag tgtaaacgcg gtattgctta agccaacaat cccggcaaag ttcggttcac
    11461 caataatcag cccacgaatt tcttcgtttt ccattaattc cgctaccgca tctttcagtg
    11521 cctgattggc tttatcagca tcttcatggc gatcaacatc gtagtttgcc actaccgaac
    11581 caatgccgcg gacaaagttg gcaaaggtgg taatcgaaga ccacggaatg atgtggtacg
    11641 ccccggtatc ctggcgtacg cccacggagc gaatcgacat ccgttccaca gtgccggtca
    11701 acggcccaat agtcaccaaa tctccagtgt tcatgccgtt ttcaaactga ataaataccc
    11761 cggtgataat atctttcacc agcgtttgcg aaccaaacga gatagccaga cctaatgccc
    11821 cggcacctgc cagcaatggc gcgatattga cgccgatttc cgacaacaca atcatgatgg
    11881 tgatggtact gataatcacc gccagcgcgt tacgaaacag cgtaagcagg gtacgcgtgc
    11941 gggcgctggg tagcgggcgg ccatgaatat ccgaagccag ccggttttcg atcaaactgg
    12001 cgagcaccgt ccagccaacc gccgagaaga aaagaatgag tgcgatacgg atcaggatat
    12061 ctacggtttt ctgccccgcg ccgttttgca gccagttcca gaaatcgaac aatccccatg
    12121 cgctcaacag cagcattacc gccacgcaga cggtcagaat acgcgccgtt ttcagcgccg
    12181 ccgaaagcca gccattcagc cgtttttgca actccggata gttacgctga gtatgtggcg
    12241 agagagtgat ggtttttgcc agccagcggg aaaacatacc ggaaacaaat gctgcgatac
    12301 caataatcgc caggctgcgc actgttgcac ccatcataaa tttcaggctg ttgcccggat
    12361 cgaacaacga aaagaaaaac agcacgataa aataggcgct tgccagccag tgccacacca
    12421 gcgcaaaggc gcggataaac aggctgaaaa aggccagcga atgctccgca aagttgagca
    12481 aatgctgggt aatctctttt ttattacgaa agatcaggta caacgcccag acggtcatgc
    12541 acagcataat gatgacgttc gccagcgcac ctatctgtac attcacctga ttagagataa
    12601 tcggcacggc cacaatcagg ccataaccta tcaaactgcc taaccagctc aggcgacgac
    12661 tccagtaacg ggcgctctcg tcctgaatcg tgaacgggcg cagttccgcc acgtttgggc
    12721 aaaaaatcag gcgtagtacg gctttgaaaa attcaatcag ggcaaaggcg ttgagaaaca
    12781 aactttgttg gaaagcgatg gtgcgactgc ctgcattcag gttgtcgctt aatatctggc
    12841 cgacaaataa tgtcagtgcc agtaacagca ggtcgataat aaacgccccg ataatcatcg
    12901 ccggaagctg cagccagtta ctgcgctcac gatttttttg ccgcgcccac tgacccattt
    12961 tgcgatacag cggcagtgcg cacaggcgaa tcagccagta aaaaccaaac actaaaaccg
    13021 ctaacattga aaaatgggtc agcgcattgc tgaaggtttg tggattaaac ggcttatgcg
    13081 gggagccggt gatattgcga taaagctgcc cgaagcgggc ggaaagggct tcaccataat
    13141 ggcggctgaa ttcggtgacc ttttgcagca cggtttgctc ttcaaccagc gtcggcggca
    13201 cgatcttcgg gaccggttca gcaggtggcg tcgcggcgac ggtgcgcaac tggtcgatca
    13261 actctttacg cgaggtgtca ttatccagca catccgccag tgcgccataa gccgcttttt
    13321 tttgttcgat atccggttcc ggggccggtt cagtcgttga gtccgttgtc gttgtggttg
    13381 taacgccggg tatggatacc gcgtgggcag gtgcgcccag caggcagaag aggatgaaca
    13441 ggatccaccg catgactcct ccagtgagaa aacagagcaa aaagataagt atagatgctg
    13501 ggggaggggt gattttaaat gagaggaatc tggtgtacct ccctttcggg tgaaagggag
    13561 gcagagaagt taagagacgt gctgtaaaaa ttcctgcaag cgctggctcg gcggattttt
    13621 aatcaacact tgtggatcgc catcttccgc aatccggcct ttgtcgataa agatcaggcg
    13681 cgaagctact ttctcggcaa aaccgatttc gtgggtcacg atcaccatcg tcatcccttc
    13741 ttcagccaga tcttgcataa ccttcagcac ttcatggcgc agttccgggt caagagcgga
    13801 agtcggttca tcaaacagca tcattttcgg cttcaccgcc agcgcgcggg caatcgccac
    13861 acgctgctgt tgaccaccag aaagttcgga agggtagtga tgtgcacgtt ctgccagacc
    13921 gactttcgcc agcagctcac gtgccagttt ttccgcctct tctttgttcg cgccacgcac
    13981 gcgtagcggg ccaaacatga cgttttccag cgccgtcaga tgcgggaaga ggtaaaactg
    14041 ctggaacacc atacctgctt cctggcgaat caggcgctcg tcaactttcg gatcgttaac
    14101 cttcaggcca tcgacaatca gatcgccgga ggtgatttct tccagtttgt tgatgcagcg
    14161 cagcagggtc gatttaccgg aaccggacgg cccgataatc accacgactt cgccctgggc
    14221 aatgttcaaa tcgatattgt gcagcacctg ggttgggcca aagtgcttgg agacgttttt
    14281 aaattcaatc acaggatttt catccttctt tccagacgac gcagaataaa gctcagcacc
    14341 agggtaataa tcagatagaa caccgccacg gcgctccaga tctcaagggc gcggaagtta
    14401 ccggcaataa tttcttgccc ctgacgggtc agttccgcca cgccgatcac aataaacagc
    14461 gaggtgtctt taatgctgat gatccactgg ttacccagcg gcggcagcat acgacgcagt
    14521 gccagcggta aaatgacgta gcgaatggtt tcccaacgtg aaagaccgag cgccagtcct
    14581 gcttcacgaa aacctttgtg gatagacagc accgcaccac gcgtgatttc cgcaatgtac
    14641 gcgccggagt tgatcatgat ggtgaccacc gccgcagtaa atgggtcgat gcgtaagtca
    14701 ttaaacgcca tcggcagggc aaaatagata aacatcacct ggacgacgat aggtgtgccg
    14761 cggatcactt caataaaaac cagcgcgacg tggttggcta tccaaccacc gaaggtgcgt
    14821 gcaaaacctg ccagcaatcc gatcaccagg ccgccagcca gaccgaggac cgatatccac
    14881 agggtcattt tggcaccttc aatcagaagc ggaatggcag gccagatagc actccagtca
    14941 aactgcatat gttgttcctg ttaccgtggt gtaaataatc aaattcaggg taattaatgg
    15001 ccccggagcg ggtttgcgcc ctcaccctaa tcctctcccc agaggggaga gggaactgcc
    15061 agtgcgcttt acaggtgtag cgttattatt tcggttcagt accgaaccat tttttgtaga
    15121 tttcgttgta agttccgttc tcgcgcaggg ttttcaacgc gccgttgact ttgtcacgca
    15181 gctcgtcgct acctttcggg aacgcaatac cgtattgctg cgcttccaga gagtcgccta
    15241 ccgctttgaa ctgaccgtta ccggcggttt tgatgaagta cagaatgttt ggcgtatcgt
    15301 gcagaacggc gtctgcgcgg ttggtgccca gttccatata ggcgttatcg atgttcggga
    15361 actgacgcag atctttagtt ttgatgtttg ctttcgcgta atcaacggag ccagtgccgc
    15421 tcttcaccgc aaccactttc ccgtcgagat ctttcacgct tttcacatcg ttattgttag
    15481 ctttcaccat cactaacagg ccgcttttgt agtagccgtc agagaaatcg atcgcttttt
    15541 tacgctcgtc ggtgatggta atgcccgcca gcgccagatc gacgtttttg gtttgcagtg
    15601 ccggaatgat cccactgaaa tccatcggct tcagttcgta atccagcttc agctctttag
    15661 cgatggcagc ccacagatca acgtcaaagc ccacatattt atcgccctgt ttaaattcaa
    15721 acggaacgaa ggcggtatcc gtcgcgacaa ctaatttttt atccgcggca tgagaagaaa
    15781 ccgcaaaagc cagggtcagt gcagccagtg aaacttttaa tacagacttc atagcatttc
    15841 cttttttcta tccacgggac gatcccctgc gtgacattca catatatgaa aaaatcgtgc
    15901 cagttttgca actcattgat ttttcgagat gcggcaaaaa cgtgatgcac gatttatagg
    15961 gcaacaccct gaagatgcac cattctgggg caccaaactg gtgcgctaaa attgtgcact
    16021 caacacagtg attatttagc gtaaaacctg atgaaaaaac aatctttatg taacgattgt
    16081 gtgatgatgt ggatacaaaa aatttactta atcagctggg gatagcagat ggatgcacta
    16141 aataagtgcg ttgaggcggg ctgcaaaaca aaacggcctc ctgtcaggaa gccgctttta
    16201 tcgggtacta aaattctgca ccatcagcga tggatttatt cgatgttaga ctcgataaac
    16261 cacaggaatt tatccaggtc gcgagacgcg gcggtcagga tatctgcggt gtcgtcatct
    16321 ttcgcttcgc caatcgcttt gcgtacgtca ttagcgacga ttgcgtaacg gtcagccagt
    16381 tctttcaggt gatcctgaac gttgtggatg tccagcgggt aacttttcag cggggttttg
    16441 ctgttgataa cttgagtggt ccccagagct acaccgccca gctgcactgc acgttctgcc
    16501 atggtatcca gatgatcgat cagtgcagtg cggaagccat ccagcatttc atgtacggca
    16561 atgaagttag cgccgcgcat gttccagtgc gcttgtttgg taatcaaaga aagatcaata
    16621 aactggataa cctggcgatt cagcaactct actgttgctt ttttctcgct gtcagagaca
    16681 tcgttgcggg tataaagcag attggtcgct tttgatttaa ctaatttagc ggtactcata
    16741 atttcatatc ctcttgatgt tatgtcccag taattaacga gactaagtat agcaccggct
    16801 atgtgttccg ctattctggc tgttcctatc acactaatag tggtaacaag cgtgaaaaac
    16861 aaaactaata aagattcaat gagttagata tattgataag aacaattctt atttatttgc
    16921 tcgccaggaa ttttcggaat aaatatgctt ttgcataatt cgatatataa agacggtgta
    16981 gaggaaaagt agcgagaaat tctgcatggt tatgcataac catgcagaaa tgtaaattta
    17041 attaatgtct aattctttta ttttgctctc tttgcgtact gtcagcgtag aacccattga
    17101 agcggcgata attgcgccga gcgccagcag ctgtatgggt gtcagtgttt ctccgaggaa
    17161 aatcatcccg gaaacggcag ccagcgccgg ttccatgctc atcagcgtac caaatgtccg
    17221 tgttggcaaa cgggtgaggg caatcatttc cagcgaataa ggcagagcgg tcgagagaat
    17281 agcgaccgcc agacccaatg gaataaccga ccagtgccag agtgcttcac cagcctgaag
    17341 cgctccaatt ggcacgaaaa ttaacgctgc aatcaacgaa ccaattgcca ccgtcgcagg
    17401 gccatgttcc gctcctgcgc gttgcccact taaaatgtaa atagcccaac aagccccggc
    17461 ccccagtgcc agcgcacagc cggttaaatc gacatgggaa acgtcttgcc ccagcggtag
    17521 caggaaccac agaccaagaa ccgccagcac aacccagacg aaatctaccg ggcgacgaga
    17581 agagaacagc gccaccgcca gtggtccggt gaactccagc gccaccgcaa tacccagcgg
    17641 taccgtctga atagagagat aaaaaagata attcatccca cccagcgaaa cgccgtaaaa
    17701 caacagcggt aaccgttgct ctttggcaaa gcgcagtcgc catggcttaa agaacgcgat
    17761 gaggatcagc gttcctaatg ccaggcgcag cgcagtgaca cccggtgcgc ccaccagagg
    17821 aaaaagtgac ttagctaacg aagctccacc ttgaatagac gccatggcaa cgagcaatat
    17881 gactattggt aaccagaccg gcattttacg taatgaacca ggcatccttt ctcccacaaa
    17941 tatctagact taagtaaagc gtggagtgta ctggatatac ccaatgctgg ttgagcattt
    18001 gttgaaaaaa ttttcccccg ttttgactaa aatgcgccag gattgatgga atcattagtc
    18061 tggtgattag gaataatctg gatgaatgac agggaaaaca tgcgtaatac ttacgcagtt
    18121 ctctgaaaaa gtgatttaaa tttagatgga tagcggtgta tggaaacgtt ctgttacatg
    18181 aaatggcccg ttagacatca caaatcgcga agagtttccc attaattttt gatatattta
    18241 aaacttagga cttatttgaa tcacatttga ggtggttatg aaaaaaattg catgtctttc
    18301 agcactggcc gcagttctgg ctttcaccgc aggtacttcc gtagctgcga cttctaccgt
    18361 aactggcggt tacgcacaga gcgacgctca gggccaaatg aacaaaatgg gcggtttcaa
    18421 cctgaaatac cgctatgaag aagacaacag cccgctgggt gtgatcggtt cattcactta
    18481 caccgagaaa agccgtactg caagctctgg tgactacaac aaaaaccagt actacggcat
    18541 cactgctggt ccggcttacc gcattaacga ctgggcaagc atctacggtg tagtgggtgt
    18601 gggttatggt aaattccaga ccactgaata cccgacctac aaacacgaca ccagcgacta
    18661 cggtttctcc tacggtgctg gtctgcagtt caacccgatg gaaaacgttg ctctggactt
    18721 ctcttacgag cagagccgta ttcgtagcgt tgacgtaggc acctggattg ccggtgttgg
    18781 ttaccgcttc taatcacttt ggtgatataa aaaatccgcc tctcggggcg gatttttgtt
    18841 tttaaggctt cgggtcgaaa atatcggttc cgagatggtt gtaatccacc ttctgtaact
    18901 ggaagttggt gatgtatatc ggcccggctt tcttctcaga tataaacggg tatttgatgt
    18961 taatttcctt cgctttaatt cccgtccact gcgagaaaaa gcctaagaag tcattggctg
    19021 agcggcgggc tttaatcacc cgatgcgctt tatcgtcgct ggaaatgacc ataaaaggca
    19081 cctggaaatt ttgctgatac ttatcatcat gggcaaggta ttgcacgtct ttgccgcgct
    19141 ctttaaaggc cagaccgtgg tcagaaaagt aaaccagcga gaagctgctg ccgctgttgc
    19201 gtaactgatc gtacagcttg cgcagtaaat cgtccgtttg cgtcatagta tagagatagc
    19261 acgacgtttc tttcgattgc acaaaggttt cgtatttgcc ttttgtccgg tcgcaggcct
    19321 gcggatgcga gcccatcaga tgcagaacaa tcagctgcgg ttgcgagtgc tcttgcgcca
    19381 gcacttgagc ggtcatatcc agtaacgctt cgtctttcgt gtttttatct gcttcaaaat
    19441 taccttcttt caggaagtac acttcatctg cacgtttggc gatgctggcg atagcagtat
    19501 cgtattcgcc gatttggccc tggttggaaa accaccaggt ctggaagccc gcgcgatttg
    19561 ccagggtgac aaagttatcc tgaaactgtg gtttgccatc gactacgcga ttgagcgtta
    19621 agccaagcga tttctgcgtg gagccactgg cggcaatgta gtcagcaaat atcaaaccgt
    19681 taacgctgct ggcaaacggg gtattgtccc agtgaccgcc aaaggcaccg agggcatcgc
    19741 gacgcgcgct ttcaccgatc accaccacat aggtctgata cttcggctta acggcagtga
    19801 ccgtccaggt atctttcatt ccggaaagtt ttgccatccg ttcttgctct tcaatcacct
    19861 cgttattatt gacgatgaca tccttagcaa aacgaaatac cggatagcca gtatctttca
    19921 gtttaaatac gccaccccag gccaggtttt gcacgggagc aacaaaaaat gtcgccacac
    19981 tgaataccag acacaggctg tcgaaggtat tccagcgcgc tttttcattc tctttttttc
    20041 gcctgattgc tattacgccg agcgcaaaaa taaataagcc gaccaggtag ctgtaccacg
    20101 gaaaaatcgt caggatttcg gtcgactctt ccatattggt ggagtgcaat gccagcaatg
    20161 tattgaaatt aggcgcgccg taggcctgag caaaagggaa ataacaagcc gccaccagcg
    20221 aactgacacc gaccagaact ttttgtacgc gaggcaatgt tcgccatagc aaaagcaaaa
    20281 tagccgtaaa cgcagcggtg tagagcaaac tgaaggggta acctaagccg aggttaatta
    20341 atagcgactg taaaaagtag aacgcagtcc acgggctaaa tacccggcta cgggtaacaa
    20401 gcgattcttt gagggttaaa ttcatatgcc actatcgtaa caaacgccat gtgcttaccc
    20461 tggcgtcaag ggtcaatacc tgcaagagtg cgtgaagagg ggaaaggggt ccgcatccgc
    20521 gagccgcaat atacgcaggg ctgcaagaag atagagcgag ccgcagttca ggtcaactac
    20581 tgacgaacaa atcttttgcg aaggggattg caaatccgac aaaaagtggg gtttttcaga
    20641 ccaaaacgcg tcgtgcctgc atgaaagcag gagtgaagcg gcgtaccgcg acgccgctat
    20701 ttatcggaag gtttatcttg ctgcggtttg ttgttgacca tatcgcacaa catagagagc
    20761 agcattaacc gtactttaaa gggagaatga ctaaacacgc gcatacacct cttgaactca
    20821 ttcataagac ctcctgactt gctaatcccg tcgatccttg agggatgatt gcattacata
    20881 cagatatagc acaggctata ttatatagct attgctaaaa cgttaatttt ttgtaccttc
    20941 gcaactctgg tttacaatgt gcgcacgaaa tgagaacgct atgtatgcgt caccataatg
    21001 aggaagcaca atgagtcgtc gcgcaggtac gccaacagca aaaaaagtga cgcagttagt
    21061 gaacgaggaa gagcacgttg aagggttccg ccaggtcaga gaggcgcatc ggcgcgagct
    21121 tattgatgat tacgttgagc tgatttctga cttgatcagg gaagtggggg aagctcgtca
    21181 ggtagacatg gctgctcgtt tgggagtttc gcaaccgacg gtggctaaaa tgcttaagcg
    21241 actggcaact atggggctga ttgaaatgat cccctggcgt ggcgtgtttt taacggcaga
    21301 aggagagaag ctggcgcagg aaagccgcga gcgacatcag atagtcgaaa atttcttgtt
    21361 ggtgttgggc gtcagtccgg aaatcgcccg tcgcgacgcg gaaggcatgg agcaccatgt
    21421 tagtgaagag acgctggatg cctttcgttt gtttacccag aaacacggtg ccaaatgagc
    21481 ctgccttttt tacgcacgct gcaaggcgat cgtttttttc agttattaat tcttgttggt
    21541 atcggattaa gttttttcgt gccctttgca ccgaaatcct ggcctgctgc tatcgactgg
    21601 cacaccatca tcaccttaag cggcctgatg ctgctgacca aaggtgtgga gttaagcggt
    21661 tattttgatg tgctggggcg caaaatggtg cgccgctttg ctacggagcg tcggctggcg
    21721 atgtttatgg tgctggcggc ggcgctgctt tctacctttc tgaccaacga tgtcacgctg
    21781 tttattgttg ttccgctgac tatcacgcta aaaaggctgt gtgagatccc ggttaatcgg
    21841 ctgattattt ttgaggcgct ggcagtcaac gctggttcgc tactgacgcc aattggcaac
    21901 ccgcaaaata ttcttatctg gggacgttct ggtctttcgt ttgccggatt tattgcccaa
    21961 atggcaccgc tggctggcgc aatgatgctg acgctcctgc tgttgtgctg gtgttgtttc
    22021 cctggaaagg cactccaata ccatacgggg gtgcaaacac cggagtggaa accgcggctg
    22081 gtgtggagtt gtctggggct gtatatcgtc tttctgacgg cgctggagtt caaacaagag
    22141 ctgtggggac tggtgattgt ggcggcgggc tttgcgctgc tggcgcgtcg cgtggtgttg
    22201 agtgtggact ggacgctgct gctggtgttt atggcgatgt ttatcgacgt ccatttactg
    22261 acccagcttc cagcgttgca aggcgtgttg ggtaacgtga gtcatttatc tgaacccgga
    22321 ttatggttaa cggcaatcgg tttatcgcag gtgatcagta acgtgccgag tactatattg
    22381 ttgctgaact atgtgccgcc gtctttatta ctggcatggg cggtaaacgt aggtggcttt
    22441 gggttattac ccggatcgct ggcaaatttg attgcgctac gtatggcgaa cgatcgccgc
    22501 atctggtggc gtttccatct ctattcaata ccgatgctgt tgtgggcggc gctggtggga
    22561 tatgttttgt tggttatgat cccggcctgg taaggtctgg caaataaaaa aggcggatta
    22621 tgagtccgcc tttgttttac gttttgaaga ttaattcaga cgaaccggca tcccggagcg
    22681 gtttttaatc gcttcatcaa gaacaacctg gtcaacatct ggctggccgg tcactgtctg
    22741 cacgctcttc gtcagggtaa ttggcacaat ttcctgacct tcaaattggg cttcggtggt
    22801 agacagcggg ttatggactt caatgtaacg gctgccgtct ggctcggtgg tcgcttttac
    22861 cggctcatca ataaactgta cgcgggtacc gaccggtact ttctcgaaca ggaatttgat
    22921 gtcttcgtta cgcagacgca cacaaccatg acttacacgc agaccgatac cgaagttagc
    22981 gttggtgcca tggatagcgt acaggcgacc gatgtagagt gcatacagcc ccatcgggtt
    23041 atccggacct gccggaacga cagccggaag cggttcgccc gcagcgcggt actctgcgtg
    23101 cattttggcg gtcggcgtcc aggtcgggcc tgctttctta cgctcaactt tggtggtcca
    23161 gttgataggc gtatctttgc ctaactgacc aatgccgatc ggcagcacga taacggtgtt
    23221 ggtccctttc ggatagtaat acagacgcat ctctgcactg ttaatgacga tgccttcatg
    23281 aacggtatcc ggcaggatca gctgctgcgg aatgttcagt acagtaccgc ctttcggcag
    23341 gaaggtatcc acacccgggt tcgcttccat catattggaa agccccatct ggtactccgc
    23401 ggcaaaatac tccagcggct gagtgttacc ttcaggaatg gtgatcacct gattctgacc
    23461 aaccaggcga ctcccgtcgg ttggcagagg ataagttacc gcagaggcgg tactgcaaaa
    23521 gccgacaaca gcgaaggccg ctgcgaataa tgttttcaat ttcatattca tgttaagcga
    23581 gattttgtgc ctggcaggcc attgggttga gaatattaga gtattggaag cgcattataa
    23641 gttcattcca gctcacagtg aaatcagatg tgtacgaaat cacatttttt gcctttggct
    23701 tgagtgtaga tcttaagcga ggagcaggat cttctttcag acttatggca taatgcgcgg
    23761 tttgtcatat ctcttttcag gatacgcctg tgttagtttc cagtaacgtc accatgcagt
    23821 tcggcagtaa gccgttgttt gaaaacattt ccgtcaaatt tggcggcggc aaccgttacg
    23881 gcctgattgg cgcgaacggt agtggtaaat ccacctttat gaagatcctc ggcggcgacc
    23941 ttgagccgac gctgggtaac gtttccctcg atcccaacga gcgcattggt aaactgcgtc
    24001 aggatcagtt tgcctttgaa gagttcactg tgctggatac ggtgatcatg gggcataaag
    24061 agttgtggga agtgaagcag gagcgcgacc gcatctatgc tttgccggaa atgagtgaag
    24121 aagacggcta taaagtggcc gatctggaag ttaaatacgg cgaaatggac ggttactctg
    24181 cggaagctcg tgccggtgaa ttgttgcttg gcgtgggaat tccagtggaa cagcactacg
    24241 gcccgatgag tgaagttgct cctggctgga agctgcgtgt gttgctggcg caggcgctgt
    24301 ttgctgatcc ggatattctc ctgctcgacg aaccgaccaa caacctcgac atcgacacca
    24361 ttcgctggct ggaacaggtg ctgaacgagc gtgacagcac catgatcatc atctcgcacg
    24421 accgtcactt ccttaacatg gtctgtaccc acatggcgga tctggattac ggcgagctgc
    24481 gcgtttatcc gggtaactac gatgagtaca tgacggcggc gacccaggcg cgtgaacgtc
    24541 tgctggccga taacgccaag aagaaagcgc agattgctga gctgcaatct ttcgttagcc
    24601 gctttagcgc caacgcctcg aaatctcgcc aggcaacttc gcgcgcgcgc cagattgata
    24661 aaatcaaact ggaagaggtg aaagcctcca gccgtcagaa cccgttcatc cgttttgaac
    24721 aggataagaa gctgttccgt aacgcgctgg aagtggaagg tctgaccaaa gggtttgata
    24781 acggtccgct gtttaaaaat ctcaacctgc tgctggaagt cggtgaaaaa ctggcggtac
    24841 tgggtaccaa cggcgtcggt aaatcaacgc tgttgaaaac gctggtgggc gatctgcaac
    24901 cggacagcgg caccgtaaaa tggtctgaaa acgcgcgcat tggttactat gctcaggacc
    24961 acgaatatga gtttgaaaat gatctgaccg tgttcgaatg gatgagccag tggaagcagg
    25021 aaggcgatga cgagcaggcg gtgcgcagta ttctcggtcg tttgctgttc agccaggacg
    25081 acatcaaaaa gcctgctaaa gtgctttccg gtggagaaaa agggcggatg ctttttggta
    25141 agttaatgat gcagaagccg aacattctga tcatggacga accgaccaac cacctggata
    25201 tggaatccat cgagtcactg aacatggcgc tggaactgta tcagggcacg ctgatctttg
    25261 tttcacacga ccgtgagttc gtaagctccc tggcgacccg cattctggaa atcaccccgg
    25321 aacgcgtgat cgactttagc ggtaattacg aagattacct gcgtagtaaa gggatcgagt
    25381 aagttattat ccgtcacccc gatactgtcg gggtgacggc ggctgatgac aaacgcaaga
    25441 ttgcctgatg cgctatgctt atcaggcatt aagaaagcgc acttgtcagc aatctgagta
    25501 acgtcgaatc gattttctta atcccacatc aattcacttc aattaactct caaacatcca
    25561 tgcccaacgc tcgcttaccg tgaatgttga ggtcggcaag cgtaaagcgt ccttcccagt
    25621 ttgtttcata gtcctcgcgc gggaagtcgc ccggcgatgc gcctttttcc agtgcggcct
    25681 tcaccttgtg cgcgtaggca agatttttct cgcacatcgg tgccgcagga atgtacatca
    25741 cgttgcccca accctgttga ttttcaacgg gggcaacgga atggatgacg tcgcagtgcc
    25801 accagacgga gtctccggcc tcgagttttg gaatgctggt taacgcctca attaacagtg
    25861 gatgccattg ctctgatacc ggcaacactc ttccgggcgc tacgccgcac agttcatcct
    25921 ccggcacatc atcaagcagc ggacgtaaca gtacgtacgc catggcttca ggaatgggca
    25981 cgacgtgcag caacccctga ctaggcagca tatcagagag cgctgtccag ccctggaatg
    26041 tccgaaacac ggaacatttg gtggtgttgt ccaccgtgta ctcttcaact tccgtacgat
    26101 gtgccgcatg ccagggatcg tatttcgcca gattgccatt aaagacgtta gcgaaaacgt
    26161 gctgatacgc tggaagcagc cagcgttcca gcgccccgga gtcggtatgc gctccaagac
    26221 ctttggaggt cgttccgggc ggacggcggc ggatacggtc agggtagatg acgctcacat
    26281 ccgggttaaa ccattgcttt ccatcacttt caaatgtcca cagacgattg agaaacgact
    26341 gcgcattcgc catttcttca ctctggcggg cctgcatttg cgcctgcgac cagtagatgg
    26401 ggtaaatctc gggacgtgaa gcgctgagcg tcccgaagaa attatcgccg gggcctttgt
    26461 agacctcgtc aaagcggttg cggtccagat agtccagcat cgactgatcc cagcctagcg
    26521 cttgttcgcg ggggaaatgg ccttttatca ccgcacaacc gcgacgttta atctgttcgc
    26581 gctgctctgc agtaacatga cctgctttga tatcggcata agacagcacc ggccagacgg
    26641 catcgccctg tgctttgagt gcgttgattt cagccactcg cgtggcaatg tcatcgctta
    26701 gctgattaaa gatctgctgg acgtcgccaa gctgcgcccg cagcgcgtgc ttcatctgac
    26761 ggatagctgc tttgtgatcg gcaggcaatg tgtcgctggt aaaagtagaa gccataacca
    26821 cctcgcatta actttcattc gaaactatgc tgatttgcat gtgacaatat aagttataaa
    26881 taagttaatg caagtttaat gatttgatgt gatgtacagg atgtaaaaag aagggataac
    26941 cggagcgcat ggctccggta gagaggtcag ctgttaaaag gggatgtgtt atccagtaca
    27001 gcctgaatca cattcagcgc gccttcatga ttattatcat cggtagcgta acgggcgatt
    27061 tgtttaatgt tttccgcagc attgcccatc gcaaaggaat aacgcgccat tttcagcatc
    27121 tccgcatcgt taccgctgtc gccaatcgct accacatttt gcggtgacag atcccagcgt
    27181 ttcagtaacc gcgaaatacc gtttgcttta tgtagaccgg gaataatcag gtcgataaag
    27241 ccaaaaccac tggtgacggg tttcataatg ccatcgagcg ctatgtgcag tttgtcgatc
    27301 actaacggga tttgttcatc cggcaggttg agtgaaaact tgaacagtac gtcgtcaatc
    27361 tcctgataat cttttacagg tttcaggcga tggtagtgtt ttgccatcag tgcgacaaat
    27421 gcttcggggg cattttcgct gacatatgca ctttgcagac cgcaggcgac aaaattgagt
    27481 tgcttatctt ttagcaactc gccaataaca atccgcgatt catgtcgggt cagttcgccg
    27541 tggaacagtt gcttgccatg ttcgtaaacc agtgcgccgt tttccgcgac aaaagagatc
    27601 tcatccttta gctcaggaaa gaatgaaata agctggtaat actgattacc gctggcgaca
    27661 acgaactcaa tgccgcgctt tttcagttcc tgatattgcg ccataaaacg tggttggttg
    27721 tacgttttga cgtcgttaag aaaagtaccg tccatatctg tgacgataac ttttacgctc
    27781 ataggtgtgc tcccggctcg aaaatgaaac cgtaacagtg taataacaat gtgacgcaga
    27841 gcacaaatta tatttcgaat gaaagtaagg atgaaattga tgatgtgaat gatttagccc
    27901 ggcgacgtct ccgccgggct gaggggatta cagcatatgt tcggtacggg cgatgatatc
    27961 gtcctgagcg tctggagaga gcgcggtgaa gaaggcggaa taacccgcga cacgcactac
    28021 cagatcgcga tactgatcgg gatgtttttt cgcctccagc agcgtttcgc gggaaacgat
    28081 gttgtactga atatgccagc ctttatgcac ttcaaagaag gtgcgcagca ggatcatcag
    28141 tttctgcttg tcagattcgt tctccagtgt tgctggattc agtttctggt tgagcaacac
    28201 cccgccgaga atcgctgccg taggcagttt acccaccgag ccaatgaccg cagtagggcc
    28261 aagatgatca gtaccggaag ccgggcttgc gccttctgcc agcggggtgt gggctttacg
    28321 tccgtctggc gttgccatag tttgcgcgcc aaacggtacg ttagcggaga tagacgacgt
    28381 acccgcgtaa tagttgccgc caaccggacc acgaccgtag cgcgggttat ggtactgttt
    28441 cagttcgtcg atataggtct gataagcgcg agccagcagc gtatcgacag tatcatcgtc
    28501 gttgccgtac ttcggcgcac cgttaatcag ccgctgacgc agctgctcgt gagtcaggcc
    28561 gtcgaagtca ttcgccagtg cggcagccag ctgttgctga ctaatcgcgc cctgttcaaa
    28621 taccagtttc ttcacggccg ccaggctgtt gccgaggttg gcaatgccaa cctgcaggcc
    28681 agaaacccag tcatatttcg cgccgccttg cttgatactt ttcgctcgct caatacagtc
    28741 atccaccagc gccgagcaga gaatatcgtg cacgttctct tccagcatgg tgtcgacgac
    28801 atattcgatt tcgattgatt tacgcgtgta gtaacggatt tgcgtatccc acgcgtccat
    28861 cacttcatcg aagttgttga agttacctgc cgacaacgct ttttcttgtg gcaggaacac
    28921 tttgccgctg gtggcatcac gcccgccttc cagcgccgcc agcatcacgc gggcgaagtt
    28981 gataaagctc atgccggtac agcgatagcc ccatttgcca ccgacggcgg tttcgataca
    29041 accaatcgct gcatagttgt aagcatcctg cggttcaata ccgagtttaa taaattccgg
    29101 gatgacgatt tcgtcgttgt tgaacgccgg catcccgaag ccgcaacgga tcacctgtac
    29161 gcaggcgtcg aggaaatcgt tgctcattcc cgcgtggtaa cgcacgctga ggttaggctg
    29221 agtggaacgc aggcgaccgc aggattcgag gatcgcgtaa gagagtggat tcaccgcgtc
    29281 cattggttga ccatcaacca gattttgtcc gccaatggtg acgttctgat acagaggact
    29341 tcccgcagag gcttttgagt gtgagcctga gcggattttg ttcacttcca gcagtttcag
    29401 ccagcagcta tgcagcaatt cgatggcgtg ttcgcgatcc agtgtctggt tgagttcaac
    29461 gtcgcggcga tagtacgggt agagatactg gtccatacga ccaaacgata ctgagtgacc
    29521 gttagattcg atctgcaaaa tcaactggat gaagtaacac agttgcagcg cctgccagaa
    29581 agtctgcggc ggctggtggg cgataagatc gcagttttct gccatcgcca gcagttcatc
    29641 gcgacggctt tcgcgggttt cggtcgcggc cattgcacgc gccagggcag cgaaacgttc
    29701 aatgtgttca ctgactgcca ccagcacgat atcaatcgct ttcaggaact gttcgccgtg
    29761 cagatcttcc agtaccgtca gattgatgcg cgagcgacgt tccgctactt tctcgcgcag
    29821 accatcaagc ctttttccag cagcagcggg aaattcaccg ccaggtgcgc atcgccggag
    29881 gtcatattgc cttccgcttt aatgattccg gtcgccagca gacctttttg ctcatcggta
    29941 aacatgccgt agcagcgatc ctgtaccgtc tgaccgcgcc accacgggca cacttcatgc
    30001 agaacgcgtt tgttctcttc gctcaccgca aagccagcac cgggacgatc tgccagatca
    30061 tcaatctctt tttcgatcca cgagacagta tattccggga agatcggcgc ggcgcgaact
    30121 tcgcttgcct ggttgccgat gatcaactca tcgtgcttga tccagatagt gcgattcgcc
    30181 aggtgatgtg ccagcgccag cgcgcgacgt acagggatcg gcttatcgag atgttgttga
    30241 tacatctcgg tatagtgctg cgcgcgctcg gtacaaaccg gcggtttcac aatatgcacc
    30301 agcgcatttt tgtgcgcttt aatgcggtcg ctgagcgtgt ccagtttcag tgtggtcatg
    30361 gttgttatcc tcgtaaggtc gcggttaacc ctttctggca ggcatactgc tgggcaaagt
    30421 cgagcagttc tggcgcatca agcggttttt ccggggcgtc atagggcaga ttaagtaagt
    30481 gatatttgtt gatgcccagc gtgtggtagg gcaggaaatg aatttcgcca acgtgcagct
    30541 cgtcggcggc aaaatcggta atggctttca cagaggtttc gtcggcatta aagccttgaa
    30601 tcagcggcac gcggataatg atttttttgc ccgctgcggc gagttttttc aggttatcca
    30661 gcaccctggc ggcgttaccg tcggtccact gtttaaacgg cgcgtcggca acgtgtttta
    30721 aatcggcaag aaacagatcg atatagggca gagaaggggc gatatatttc cacggcacat
    30781 gcaggcaggt ttctaccgca gtatgaatgc cggcctcgtg gctggcttgc agtagcgccg
    30841 tcgccatttc cggctgcatg aatggttcac cgccagaaag tgttaagccg ccgccgctgc
    30901 gatcgtaaaa cggtttatcg cgcagaacgg tcaccatgat ctcctcaacg cttttcactt
    30961 ccccacacac ggttaacgac tgtgtcgggc agcagtcggt taacgccgtc agatgctccg
    31021 gggttaactt ttcccgatga ataagcaaac cattcagcgc gcgctcaatc acttccggcg
    31081 cggtcttagc gcacagctca cagccttcca ggcacagacg tgggtcatac agcagatcct
    31141 gcgtgcgggc gcggctttcc gggttctgac accagcggca gcccagcgaa cagcctttaa
    31201 gaaatacgac cgtgcggata ccggggccat catgggtcga gtagcgctga atattgaaaa
    31261 tcatagttgc ctctctgttt cgttcaaata ttaaaatgct ttcgaatgaa agttagattg
    31321 atgtgcgtca actgttcaga gagttttccc gtgatagtct acattcagac aaaaagtaca
    31381 ttttgaggat ggttatggaa ctgtatctgg atacttcaga cgttgttgcg gtgaaggcgc
    31441 tgtcacgtat ttttccgctg gcgggtgtga ccactaaccc aagcattatc gccgcgggta
    31501 aaaaaccgct ggaggttgtg cttccggaac ttcatgaagc gatgggcggt caggggcgtc
    31561 tgtttgccca ggtaatggct accactgctg aagggatggt gaatgacgca cgtaaattgc
    31621 gttctattat tgcggatatc gtggtgaaag ttccggtgac caccgagggg ctggcagcta
    31681 ttaagatgtt aaaagcggaa gggattccga cgctgggaac cgcggtatat ggcgcagcac
    31741 aagggctgct gtcggcgctg gcaggtgcgg aatatgttgc gccttacgtt aatcgtattg
    31801 atgctcaggg cggtagcggt attcagactg tgaccgactt acaccagtta ttgaaaatgc
    31861 atgcgccgcg ggcgaaagtg ttagccgcga gtttcaaaac cccgcgtcag gcgctggact
    31921 gcttactggc aggatgtgaa tcaattactc tgccactgga tgtggcacaa cagatgatta
    31981 gctatccggc ggttgatgcc gctgtggcga agtttgagca ggactggcag ggagcgtttg
    32041 gcagaacgtc gatttaaccg gacgttctgc atcctcataa attgctgatg acgtggcgga
    32101 gtgcaccgat gcctgccgcg cttggaaatc tgtgcccgaa ccgtaggcca gataaggcgt
    32161 tcacgccgca tctggcgtct ggagcaaatg cctgatgcga cgcttgtcgc gtcttatcag
    32221 gcctggaggt ggcaattact gcccgcacac ctcacacccc ggattgcgca tcagtttcat
    32281 ttcgcgaaac tgacaggtca tcgcatcgta catgacgatt ttcccgctgg ccggtttgcc
    32341 ataacctgcc agcagtttga tcgcttccat tgcctgcaac gaaccaatca cgccgatcaa
    32401 cggtgccatc acgcctgctt ctacgcaggt taacgcgttc tcgctaaaca aacggctaag
    32461 gcagcgatag cacggttcgc cgtcctgata agtgaagacc gtgatttgcc cttccatacg
    32521 aattgccgcg ccggaaacca gcggcacctt cgcggcaaaa cagcctgcgt tcagttgatt
    32581 acgtaccgca acgttatccg tacagtcgag cacaagattg tgttcagcaa tcaatgctgc
    32641 aagttctgcg tcatccagca gtgcattgac tggcgtaatc gcgatatgtg ggttgatccg
    32701 cgtcagagcg tcacgggcgg attccacctt cggttgcccg accgtagcat cactgtgcag
    32761 cgtctggcgt tgcagattcg agagcgaaac cgagtcgaag tcgagcagcg tcaggttgcc
    32821 gacaccggcg cttgccagat actgcgaagc tgcgcagccg agtccaccga ggccgacgac
    32881 cagcacacga gaatctttca gcgcctcctg accgtcaaaa tcaaaaccgc gcagaatgat
    32941 ttgccggttg tagcgcagca tctcttgatc gctgagttcc gccattacag gcctccgaac
    33001 aacgcgttaa acggttctac ttccacccat tcgcccactt ccacattgcc gcgatcgcgt
    33061 tccagcacga taaagcagtt gccgaggcta aaggagctga atatatgtga accctgatgt
    33121 ccggtggtcg tcacttccag ttcgccatcg gcgttgcggt gcagcacgcc gcgctggaaa
    33181 tcaagacgtc ctggcgtttt tttcaggcgg gacgctgtgc gtacgcgctg gcgcgcgggc
    33241 aggccgctgg cggtgttgcc gcttagtttt gccagcaaag gctgtaccag ttgatagaag
    33301 gtcagcgtcg ctgaaaccgg gttgcccggc aggccgcaga accagctatt gctgagttta
    33361 ccgaacgcga acggtttacc tggtttaatc gccagcttcc agaaggcgat ctcccccagc
    33421 tcttcaagaa tcgttttggt gtaatccgct tcacccactg aaacaccgcc ggaactgatc
    33481 accacatccg cctggctgtc ggcttcaata aatgcggcgc gcagggcatg tggatcgtcg
    33541 cggataatcc ctaagttgat tacctcgcaa cccagctgtt ctaacatcag gtgtacggcg
    33601 agacggttgg tatcgtagat ttggccgtcg cccagcggct ggcctggcaa ctggagttca
    33661 tcaccggtag aaaaaagcgc tacacgcact ttacgaatca ccggaacttc ggcaatcccc
    33721 agtgaagcaa ttactggcag ctctgcggta gtcaggcgag tccccgccgg gaaaacaacc
    33781 gcacctgcag agatatcttc accgcgacgg cgaatatttt gcccgctacg cacttcagca
    33841 gtaaaacgca cgccattgtc cgtttgttca gtctgctcct gcatcaccac cgcttcgcag
    33901 ccttccggca ccggcgcacc ggtcataata cgaatgcagg tacccgcagg ccattcacca
    33961 tggtacggct gaccggcaaa ggatttaccg gcaacgggca gcggttgtcc ggaggtgata
    34021 tcggccaaac gtaccgcgta accatccatt gcggagttat caaaccctgg gacatccagc
    34081 ggcgaaacga catcgctcgc cagaatacga ccaaaacact gaaccagtgg cagcgtttcc
    34141 tgggcggtca gtggggtgac gcgagaaagc atctcattaa gcgcggtgtc gagcgacatc
    34201 aatccggtgg taaattccat gaaaacactc ctgcggaggc aaaatcgaat ttgcctatta
    34261 tgtcagaaaa acgccacaga ctgtatgcca cctcgggcgt agcgctgggt cctgccttta
    34321 catgccatat ccatctttct atattcaaaa attgaatgag taattcataa aaattctgat
    34381 atttatagca aaagtggcga gccaccctta atggacgaat actatgggca aagcagtcat
    34441 tgcaattcat ggtggcgcag gtgcaattag ccgcgcgcag atgagtctgc aacaggaatt
    34501 acgctacatc gaggcgttgt ctgccattgt tgaaaccggg cagaaaatgc tggaagcggg
    34561 cgaaagtgcg ctggatgtgg tgacggaagc ggtgcgtctg ctggaagagt gtccactgtt
    34621 taacgccgga attggcgccg tctttacgcg tgatgaaacg catgaactgg acgcctgtgt
    34681 gatggatggt aacaccctga aagccggtgc ggtggcgggc gttagtcatc tgcgtaatcc
    34741 ggttcttgcc gcccggctgg tgatggagta aagcccgcat gtgatgatga ttggcgaagg
    34801 ggcagaaaat tttgcgtttg ctcatggcat ggagcgcgtc tcaccggaga ttttctccac
    34861 gcctttgcgt tatgaacaac taatggcagc gcgcgaggaa ggggcaacag tcctcgacca
    34921 tagcggtgcg ccactggatg aaaaacagaa aatgggcacc gtgggggccg tggcgttgga
    34981 tttagacggc aatctggcgg cagccacgtc cacgggcgga atgaccaata aattacccgg
    35041 acgagttggc gatagcccct tagtgggtgc cggatgctac gccaataacg ccagtgtggc
    35101 ggtttcttgt accggcacgg gcgaagtctt catccgcgcg ctggcggcat atgacatcgc
    35161 cgcgttaatg gattacggcg gattaagtct cgcggaagcc tgcgagcggg tagtaatgga
    35221 aaaactccct gcgcttggcg gtagcggtgg cttaatcgct atcgaccatg aagggaatgt
    35281 cgcgctaccg tttaacaccg aaggaatgta tcgcgcctgg ggctacgcag gcgatacgcc
    35341 aaccaccggt atctaccgtg aaaaagggga caccgttgcc acacagtgat gaacttgatg
    35401 ccggtaatgt gctggcggtt gaaaatctta atattgcctt tatgcaggac cagcagaaaa
    35461 tagctgcggt ccgcaatctc tcttttagtc tgcaacgcgg tgagacgctg gcaattgttg
    35521 gcgaatccgg ctccggtaag tcagtgactg cgctggcatt gatgcgtctg ttggaacagg
    35581 cgggcggttt agtgcagtgc gataaaatgc tgttgcggcg gcgcagtcgt gatgtgattg
    35641 aacttagcga gcagagcgct gcacaaatgc gccatgtgcg cggtgcggat atggcgatga
    35701 tatttcagga gccgatgaca tcgctgaacc cagtatttac tgtgggtgaa cagattgccg
    35761 aatcaattcg tctgcatcag aacgccagtc gtgaagaagc gatggtcgag gcgaagcgga
    35821 tgctggatca ggtacgcatt ccggaggcac aaaccattct ttcacgttat ccgcatcaac
    35881 tctctggcgg gatgcgccag cgagtgatga ttgcgatggc gctgtcatgc cgcccggcag
    35941 tgctgatagc cgatgagcca accaccgcgc tggatgtcac tattcaggcg cagatcctgc
    36001 aattaatcaa agtattgcaa aaagagatgt cgatgggcgt tatctttatc actcacgata
    36061 tgggcgtggt ggcagagatt gccgatcggg tactggtgat gtatcagggc gaggcggtgg
    36121 aaacgggtac cgtcgaacag atttttcatg caccgcaaca tccctacacc cgtgcgctgt
    36181 tagctgctgt tccgcaactt ggtgcgatga aagggttaga ttatccccga cgtttcccat
    36241 tgatatcgct tgaacatcca gcgaaacagg ccccccccat cgagcagaaa acggtggtgg
    36301 atggcgaacc tgttttacgg gtgcgtaatc tggtcacccg tttccctttg cgcagcggtt
    36361 tgttgaatcg cgtaacgcgg gaagtgcatg ccgttgagaa agtcagtttt gatctctggc
    36421 ctggcgaaac gctatcgctg gtgggcgagt ctggcagcgg taaatccact accgggcggg
    36481 cgttgctgcg cctggtcgaa tcgcagggcg gcgaaattat ctttaacggt cagcgaatcg
    36541 ataccttgtc acccggcaaa cttcaggcat tgcgccgcga tattcagttt atttttcagg
    36601 acccttacgc ttcgctggac ccacgtcaga ccatcggtga ttcgattatc gaaccgctgc
    36661 gcgtacacgg tttattgcca ggtaaagaag cggttgcacg cgttgcgtgg ttgctggagc
    36721 gcgtgggcct gttacctgaa catgcctggc gttacccgca tgagttttcc ggcggtcagc
    36781 gccagcgcat ctgcattgct cgcgcgttgg cattgaatcc aaaagtgatc attgccgacg
    36841 aagccgtttc ggcgctggat gtttcaattc gcgggcagat tatcaacttg ttgctcgatc
    36901 tccagcgtga tttcggcatt gcgtatctgt ttatctccca cgatatggct gtggtagagc
    36961 ggattagtca tcgtgtggcg gtgatgtatc tcgggcaaat tgttgaaatt ggtccacggc
    37021 gcgcggtctt cgaaaacccg cagcatcctt atacgcgtaa attactggcg gcagttccgg
    37081 tcgctgaacc gtcccgacaa cgaccgcagc gtgtactgct gtcggacgat cttcccagca
    37141 atattcatct gcgtggcgaa gaggtggcag ccgtctcgtt gcaatgcgtc gggccggggc
    37201 attacgtcgc acaaccacaa tcagaatacg cattcatgcg tagataacat tcaggcggag
    37261 aataaaatgg caagagctgt acaccgtagt gggttagtgg cgctgggcat tgcgacagcg
    37321 ttgatggcat cttgtgcatt cgctgccaaa gatgtggtgg tggcggtagg atcgaatttc
    37381 accacgctcg atccgtatga cgcgaatgac acgttatctc aggccgtagc gaaatcgttt
    37441 taccaggggc tgttcggtct ggataaagag atgaaactga aaaacgtgct ggcggagagt
    37501 tataccgttt ccgatgacgg cattacttac accgtgaaat tgcgggaagg aattaaattc
    37561 caggatggca ccgatttcaa cgcagcggcg gtaaaagcga atctggaccg ggccagcgat
    37621 ccggcgaatc atcttaaacg ctataacctg tataagaata ttgctaaaac ggaagcgatc
    37681 gatccgacaa cggtaaagat taccctcaaa cagccgttct cagcgtttat taatattctt
    37741 gcccatccgg cgaccgcgat gatttcaccg gcagcgctgg aaaaatatgg caaggagatt
    37801 ggttttcatc cggtgggaac cggaccgtat gaactggata cctggaatca gaccgatttt
    37861 gtgaaggtga aaaaattcgc gggttactgg cagccaggat tgcccaaact ggacagcata
    37921 acctggcgtc cggtggcgga taacaacacc cgcgcggcaa tgctgcaaac cggtgaagcg
    37981 cagtttgctt tccccattcc ttacgagcag gccgcactgc tggagaaaaa caaaaatatc
    38041 gagttgatgg ccagtccgtc aattatgcag cgttatatca gtatgaacgt gacgcaaaag
    38101 ccattcgata acccgaaggt ccgtgaggcg ctgaattacg ccattaaccg tccggcgctg
    38161 gtgaaagtgg ccttcgcggg ctatgcaacg ccagctactg gtgtggtacc gccgagtatc
    38221 gcctacgcgc aaagttataa accgtggcct tacgatccag tgaaagcgcg cgaattactg
    38281 aaagaggcgg gatatcccaa cggtttcagt accacgctgt ggtcgtcaca taaccacagc
    38341 accgcgcaga aagtgctgca atttacccag cagcagttag cgcaggtcgg gattaaagcc
    38401 caggtgactg cgatggatgc cggacagcgg gcggcagaag ttgaaggtaa agggcaaaaa
    38461 gagagcggcg tgcggatgtt ctacactggc tggtcggctt caaccggcga agcggactgg
    38521 gcactatcgc cgttgtttgc ctcgcaaaac tggccaccga cgctgtttaa taccgcgttt
    38581 tacagcaata aacaggtgga tgacttcctg gctcaggcac tgaaaactaa tgatccggcg
    38641 gaaaagaccc gcttatataa ggcggcgcag gatatcatct ggcaagagtc gccgtggatc
    38701 ccgctggtgg tagaaaaact ggtgtcggca cacagtaaaa acctgaccgg tttttggatc
    38761 atgccagata ccggcttcag ctttgaagac gcggatttgc aataagcaac gcagggagtg
    38821 gaatgcttaa ttacgttatc aaacgattac tggggttgat tccgacgctg tttatcgtct
    38881 tggtgctggt gtttttattt gtgcatatgc tgcccggcga tccggcgcga ttgattgccg
    38941 ggcccgaagc tgatgcgcag gttatagaac tggtgcgtca gcagctgggg ttggatcagc
    39001 cgctgtatca ccagttctgg cactatatca gcaatgctgt gcagggggat tttggcctgt
    39061 cgatggtgtc acgtcgtccg gttgccgatg agattgccag gcgctttatg ccaacgctgt
    39121 ggctgaccat aaccagtatg gtctgggcgg tgatatttgg tatggcggcg ggaattatcg
    39181 ccgccgtctg gcgtaaccgt tggccggatc gattgagtat gaccattgcg gtgtcgggga
    39241 tctcgtttcc ggcatttgcg ctggggatgc ttttaattca ggtattctcc gttgaactgg
    39301 gctggctgcc taccgtggga gcagacagtt ggcagcacta cattttaccc tccctgacgc
    39361 tcggcgcggc agtggccgcc gtgatggcgc gctttacccg cgcgtcgttt gtcgacgttt
    39421 taagcgaaga ttatatgcgt accgcgaggg cgaaaggggt gagcgaaacc tgggttgtcc
    39481 tcaaacacgg gctacgcaac gcgatgatcc cggtagtgac catgatgggc ttacagtttg
    39541 gctttttgct cggtggttcc atcgtggtgg agaaagtttt caactggccc ggacttggac
    39601 gcttactcgt tgactccgta gaaatgcgcg attacccggt gattcaggcg gaaattctgc
    39661 ttttctcgct ggaatttatt cttatcaact tagtggtgga tgtgctttac gccgccatta
    39721 acccggctat caggtacaag taaggatgcg actatttaac tggcgacgtc aggcggtgtt
    39781 aaacgccatg ccactggtca aacctgacca ggtacgtaca ccgtggcatg aattctggcg
    39841 acgatttcgc cgtcagcata tggcgatgac cgccgcatta ttcgttattt tattgattgt
    39901 ggtggccatt tttgcacgct ggatcgctcc ctatgacgcc gaaaattatt ttgattatga
    39961 caatctgaat aacggacctt ctttgcagca ctggtttggc gtcgattcac tggggcgcga
    40021 cattttcagc cgtgtcctgg ttggtgcgca aatctcgctg gcggcgggcg tgtttgccgt
    40081 gtttatcggt gcggcgatag ggacgttgct gggcttgttg gctggatatt atgaaggctg
    40141 gtgggatcgg ctgatcatgc gcatttgcga tgtgctgttt gccttcccgg gtattttact
    40201 ggcgatcgct gttgttgcgg tgttgggaag cggcattgct aacgtgatta ttgcagtcgc
    40261 cattttttcc atccctgcgt ttgcccgcct ggtgcgcggc aacacgctgg tgttgaaaca
    40321 gcaaaccttt attgagtcag cacgcagtat tggtgccagc gatatgacca ttttgttgcg
    40381 tcatatcctg cctgggaccg tctcttctat cgtggtgttt ttcaccatgc gcattggtac
    40441 ctcgattatc tctgccgcca gcctgtcatt tctcggcctc ggtgcgcagc cgccgacacc
    40501 agagtgggga gcaatgctca atgaggctcg agcggatatg gttatcgcgc cgcatgtcgc
    40561 tgtttttccg gccctggcta tttttctgac tgtactggcg ttcaatttgt tgggcgatgg
    40621 tttacgcgat gcgctggatc cgaaaattaa aggatagtta cgtttgaata ttgcttgaaa
    40681 gagtaatcac ctcacaggaa attattgccc taagcaagtg ttgtaacttt ctgctgattt
    40741 tgtagaatcg ggtaatttgg ttaaatagcc gcagcaaggg actgtttttg cagcggcaca
    40801 gcgttcagac agttattttg ttaaatgtat taacatgctg agtttatacg aaaagataaa
    40861 gataaggctg at
//
LOCUS       DAZKFZ010000018        40054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000018 DAZKFZ010000000
VERSION     DAZKFZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHOQW_000901"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHOQW_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP9572019.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHOQW_000902"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHOQW_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP9572020.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHOQW_000903"
     CDS             complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHOQW_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP9572021.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHOQW_000904"
     CDS             complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHOQW_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9572022.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHOQW_000905"
     CDS             complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHOQW_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP9572023.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHOQW_000906"
     CDS             complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHOQW_000906"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP9572024.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHOQW_000907"
     CDS             complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHOQW_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP9572025.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHOQW_000908"
     CDS             complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHOQW_000908"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP9572026.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHOQW_000909"
     CDS             complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHOQW_000909"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP9572027.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHOQW_000910"
     CDS             complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHOQW_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP9572028.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHOQW_000911"
     CDS             complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHOQW_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP9572029.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHOQW_000912"
     CDS             complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHOQW_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP9572030.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11268..12308
                     /gene="gpr"
                     /locus_tag="ACHOQW_000913"
     CDS             11268..12308
                     /gene="gpr"
                     /locus_tag="ACHOQW_000913"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP9572031.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHOQW_000914"
     CDS             complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHOQW_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP9572032.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13033..13917
                     /gene="yghA"
                     /locus_tag="ACHOQW_000915"
     CDS             13033..13917
                     /gene="yghA"
                     /locus_tag="ACHOQW_000915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9572033.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHOQW_000916"
     CDS             complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHOQW_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP9572034.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHOQW_000917"
     CDS             complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHOQW_000917"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9572035.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15607..16794
                     /gene="metC"
                     /locus_tag="ACHOQW_000918"
     CDS             15607..16794
                     /gene="metC"
                     /locus_tag="ACHOQW_000918"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP9572036.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16934..17593
                     /gene="yghB"
                     /locus_tag="ACHOQW_000919"
     CDS             16934..17593
                     /gene="yghB"
                     /locus_tag="ACHOQW_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP9572037.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHOQW_000920"
     CDS             complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHOQW_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP9572038.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHOQW_000921"
     CDS             18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHOQW_000921"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9572039.1"
                     /translation="MNNFNLHTPTRILFGKSAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHOQW_000922"
     CDS             19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHOQW_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP9572040.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21021..21943
                     /gene="yqhG"
                     /locus_tag="ACHOQW_000923"
                     /pseudo
     CDS             21021..21943
                     /gene="yqhG"
                     /locus_tag="ACHOQW_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            21992..22249
                     /gene="yqhH"
                     /locus_tag="ACHOQW_000924"
     CDS             21992..22249
                     /gene="yqhH"
                     /locus_tag="ACHOQW_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP9572041.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22292..24511)
                     /gene="ygiQ"
                     /locus_tag="ACHOQW_000925"
     CDS             complement(22292..24511)
                     /gene="ygiQ"
                     /locus_tag="ACHOQW_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP9572042.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24622..26034)
                     /gene="ftsP"
                     /locus_tag="ACHOQW_000926"
     CDS             complement(24622..26034)
                     /gene="ftsP"
                     /locus_tag="ACHOQW_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP9572043.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26109..26846)
                     /gene="plsC"
                     /locus_tag="ACHOQW_000927"
                     /gene_synonym="parF"
     CDS             complement(26109..26846)
                     /gene="plsC"
                     /locus_tag="ACHOQW_000927"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP9572044.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27080..29338)
                     /gene="parC"
                     /locus_tag="ACHOQW_000928"
     CDS             complement(27080..29338)
                     /gene="parC"
                     /locus_tag="ACHOQW_000928"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9572045.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29476..31083)
                     /gene="ygiS"
                     /locus_tag="ACHOQW_000929"
     CDS             complement(29476..31083)
                     /gene="ygiS"
                     /locus_tag="ACHOQW_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9572046.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31192..31674)
                     /gene="ygiV"
                     /locus_tag="ACHOQW_000930"
     CDS             complement(31192..31674)
                     /gene="ygiV"
                     /locus_tag="ACHOQW_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9572047.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31727..32119)
                     /gene="ygiW"
                     /locus_tag="ACHOQW_000931"
     CDS             complement(31727..32119)
                     /gene="ygiW"
                     /locus_tag="ACHOQW_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP9572048.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32271..32930
                     /gene="qseB"
                     /locus_tag="ACHOQW_000932"
     CDS             32271..32930
                     /gene="qseB"
                     /locus_tag="ACHOQW_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP9572049.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32927..34276
                     /gene="qseC"
                     /locus_tag="ACHOQW_000933"
     CDS             32927..34276
                     /gene="qseC"
                     /locus_tag="ACHOQW_000933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP9572050.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34386..34968
                     /gene="mdaB"
                     /locus_tag="ACHOQW_000934"
                     /pseudo
     CDS             34386..34968
                     /gene="mdaB"
                     /locus_tag="ACHOQW_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
     gene            34999..35313
                     /gene="ygiN"
                     /locus_tag="ACHOQW_000935"
     CDS             34999..35313
                     /gene="ygiN"
                     /locus_tag="ACHOQW_000935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP9572051.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35358..36245)
                     /locus_tag="ACHOQW_000936"
     CDS             complement(35358..36245)
                     /locus_tag="ACHOQW_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9572052.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36242..37189)
                     /locus_tag="ACHOQW_000937"
     CDS             complement(36242..37189)
                     /locus_tag="ACHOQW_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9572053.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37200..38237)
                     /locus_tag="ACHOQW_000938"
     CDS             complement(37200..38237)
                     /locus_tag="ACHOQW_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9572054.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38246..39229)
                     /locus_tag="ACHOQW_000939"
     CDS             complement(38246..39229)
                     /locus_tag="ACHOQW_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9572055.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39226..40035)
                     /locus_tag="ACHOQW_000940"
     CDS             complement(39226..40035)
                     /locus_tag="ACHOQW_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9572056.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT         9968 a        10778 c        10055 g         9253 t
ORIGIN      
        1 ataatcagta cgggattgct acaacatgta ttactctttc accacaatta acggttcaat
       61 atcactctct tttttaataa ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttacca ctggttttgt ccagcacctc
      241 ttcatgatgg ctgacgaggt cgatgttgct gccacagcga ccggcgatcg gtttcactgc
      301 gtaacctgtt ttcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcatt
      481 ttgcggatga ccggtacgga tcggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacag gaggcggagt cggcgttgta
      901 ctcgtaaacc ttcagaccac gctcatccat gcagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa tcagctcctg ctcggcgctc tcggtaatgg tgtagtaatg
     1141 atacggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gcagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccagacgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt
     1381 ttcgaccacc atctccagtt cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgtaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttcacg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 gttcagaaag agaaaacggc gagcaaattc aacgcattgc catttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc ggtgggcaaa
     2221 catccattac aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tcgcgctggg cgttactggc gcagagtacg atgcctggct gatacgcata
     2341 ggcgatggtg atcaattctc cagcggcttt gtcgaagtga acccaaattc gaagatcccg
     2401 gcgctgcgcg atcataccca taatccgccg atccgcgtgt ttgagtcagg atcgattctg
     2461 ctttatctgg cggagaaatt tggctacttc ctgccgcagg atctggcaaa acggactgaa
     2521 acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gagtacgcca tcaatcgctt taccatggaa
     2641 gccaagcgcc tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc
     2761 ggcgtgtatg atgccgcaga gtttctggat gcgggaagtt ataaacatgt gcaacgctgg
     2821 gcgaaagaag tgggcgaacg tccggcagtg aagcgtgggc gtattgttaa ccgcaccaac
     2881 gggccgctga acgagcagtt gcatgagcgc catgacgcca gtgattttga gacgaatacg
     2941 gaagataagc gtcaggggta aggattggtg ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgagc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcagaccta cgaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt
     3121 gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt
     3181 accagcaccc actggcccag cagatcggca gggttacctt cacaaatcag ggcgatattc
     3241 acgtcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca
     3301 gccagcacct ggcctggaac gccaatacac attgttaact ccggttattc aacttcaata
     3361 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga
     3421 cactgcgcat cgtgctgatg aacctccacc acctggctgc aatcccagca ccaggcctgg
     3481 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
     3541 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac
     3601 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gctggataat ttcaacggcg
     3661 ctctggcaaa gcgacaactc atgcattttc gccactcctg cgaccaaaca gcagggcgcg
     3721 acgccctgcg tgtggtacat ccggattcgt gactggcagc gaaaggatca ttcgtgcgca
     3781 gtcatccgtc aggcgttggc cctcttcaat cgacatactg tgcgaaagcg gtgacatcag
     3841 cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc
     3901 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
     3961 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
     4021 ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagagc
     4081 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
     4141 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgccag
     4201 attcacgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg
     4261 gcgttaagcc gatgtgtggc tccagcgatt ccggaattac gccgaccagg gtcagctttt
     4321 tcggaaactc gccggtgaag cgcagggccg acaagacgtc ggccaggcca agctgatgcg
     4381 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
     4441 ccggcgcgct cttcttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca
     4501 tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
     4561 gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc gatggcttca tcggtcagca
     4621 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
     4681 gttgccgtca gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
     4741 ggtacgcacc acttccagcg gtttattcgg atcagcaacc ggtgtaccca ccagcgactg
     4801 ctcgtaagga ccgacgtcat cattgaagtt acgcgggcca gagttccagg ttgatggaac
     4861 aactgcctgg tagttgctga tgataccgtc tttgatcacc atccagtgag agagcatacc
     4921 gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt
     4981 cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg
     5041 caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc ccagcgtgga
     5101 gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc
     5161 attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag
     5221 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc
     5281 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggtg cctgcggcgc
     5341 ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc
     5401 ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt agccgcccgg
     5461 gaacaggaag ctgccgtttt tactgtcggt cgggaattcc ggcacgctca ggtagttcac
     5521 cgcaccttta ccgcgcgtca gccattccgg gtagaacgca gcgataaccg cagtatcgac
     5581 cttgtaaacc tgctcaacaa agtcactcag cttgtcgatg aaagacttga tgtacatcag
     5641 gcgctcaagg ttcagcacgc ccagaccgtc gaggttgatt gggttagcga ccccacctac
     5701 cgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
     5761 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
     5821 cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact gaccgctggc
     5881 aaccagatct ttgatcttgt tctgaacttt ggtgaactct tccggactgt tcaggtgcca
     5941 ggtcgaaaca cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt
     6001 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
     6061 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
     6121 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
     6181 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
     6241 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
     6301 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
     6361 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
     6421 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
     6481 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag
     6541 gtcagacgcc aggttgcaca acctaacagc gcgctcagtg ctgacaggaa cagcatgcgg
     6601 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
     6661 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
     6721 tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg
     6781 gtcagcttga cgaataggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
     6841 agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
     6901 agcggcaaca tttcatagct ctgccacagc ggatgcacct tgtagcccgc cgagatcatc
     6961 agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag
     7021 aacatcacct tgttcagacg ctgtagcgac accttccagc ccagacgttc aaacagtgcc
     7081 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
     7141 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
     7201 cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc
     7261 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgcaagc
     7321 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
     7381 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
     7441 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
     7501 ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
     7561 tttggtgttg cgacgaacca gcacggtcaa gcccgccagc acagccagtg gtagcatcat
     7621 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
     7681 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
     7741 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
     7801 gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
     7861 ttttttcgcc tccgccatca gctcttcacg cgtaccaaaa atcaccgcgc ccgccgggca
     7921 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
     7981 cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
     8041 cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat
     8101 gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc
     8161 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
     8221 gacccctgtg ccgctggtcc acacttgaat gatgttattg gtatacggcg acagtttgtc
     8281 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca
     8341 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
     8401 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
     8461 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
     8521 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
     8581 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg
     8641 ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
     8701 gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc atagcacggg
     8761 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
     8821 ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
     8881 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
     8941 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
     9001 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
     9061 cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac tgcgccagtt
     9121 gggttaactc cagctgcggc aacaccgccc cacgcagagc aggaaccgat agcgataatg
     9181 gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
     9241 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
     9301 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
     9361 agcacttcgt gatactccag agagatagtc tccagtacga ggttttctac cgttggatgc
     9421 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
     9481 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
     9541 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
     9601 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat
     9661 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
     9721 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
     9781 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
     9841 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
     9901 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
     9961 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    10021 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    10081 agcctttacc aaaatggtta ttcatcgttc acctcattcg ctatatatcg taatatatag
    10141 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatgctt gtttaagacg
    10201 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    10261 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    10321 tgattaaccc ccggatagat atacgcctca taaaccttat tattggcttt caacgccgcc
    10381 tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    10441 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcactgcg
    10501 caggccagtt ccgggtaagc gaccgctgcc gcgttcgata cgccaccgcc atagcaaaat
    10561 ccggtaatac ccactttgcc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    10621 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10681 tcatttcccg gataacctcc aacggaattt aagccgtcag gtgccagggc gatatacccc
    10741 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    10801 accaccacca cggctggcgt tttgccgctc atttttgcgg gtttaaccag ataaccccgt
    10861 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaaaaat ctccgggtcg
    10921 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    10981 gccgtcatcc cgcctaccgc atacttcgcc gcaaggttga ggaactcacg tttcgagatt
    11041 ttcccgtgag cgtaatagtc gtagtaatcc agcaactctt gtgggaaatc tttggcggtt
    11101 aaacgcggca tcgttgcact cctcagttgg tgttttttta agcaaagcat aagcacgtgt
    11161 ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc
    11221 taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag
    11281 cgaatcccga acgttacggg cagatgcaat accgctattg cggaaaaagc ggtttacgcc
    11341 tgcccgcgtt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    11401 agcgtgcgat cctgcgtaaa gcgtttgatt taggcattac gcactttgat ttagccaaca
    11461 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgggaggatt
    11521 ttgccgctta tcgcgatgaa ctgattatct ctaccaaagc tggctacgat atgtggcccg
    11581 gcccttacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    11641 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    11701 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    11761 tcgggatctc ctcttactca ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt
    11821 ggaaaattcc gctgttaatt catcaacctt cgtacaattt actaaaccgc tgggtggata
    11881 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgcattgcc tttactcctc
    11941 tggctcaggg attgctgacc ggaaaatatc tcaacggcat tcctgaagat tcacggatgc
    12001 atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gctcaccgaa gccaacctca
    12061 acagcctacg cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    12121 cgttaagctg gttgctgaaa gatgagcgag tgacgtcggt attggttggt gccagccgcg
    12181 cggagcaact tgaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc
    12241 tggcgcagat cgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg
    12301 ataaatgacc tgttaataac gggcgcgaga accgcgcccg aatcagatca gtgattatga
    12361 cgagtcagtc ggtcaagata gcccattaca aatgcagaga gcacaaacgt cagatggata
    12421 atgacgtacc acatcagttt gttatcaggg acatttttcg catccataaa gacgcgcagt
    12481 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgca
    12541 tccattttcc ccagccagtt cagcttctct ttgttctcgg agatatcgag ttgtgagacg
    12601 aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    12661 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    12721 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    12781 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    12841 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcgc
    12901 gcaacagcaa atcaacatat ccgttaaatc tttgtcgcat aattctgagg gttagggatc
    12961 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    13021 gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat
    13081 cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccggt accggattgc
    13141 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagccct ggtgacaggg
    13201 ggcgattccg gaatcggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg
    13261 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa
    13321 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa atttgcccgt
    13381 tcgctggttc acgaagcgca taaggcctta ggtgggctgg atattatggc gctggtcgcc
    13441 gggaaacagg ttgccattcc tgatattgca gacctcacca gcgaacagtt tcaaaagacc
    13501 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    13561 aaaggtgcaa gtattatcac cacttcgtca atccaggcat accagccaag cccgcattta
    13621 ctggactatg cagctacgaa ggcggcgatt ctgaactaca gccgaggcct ggcaaaacag
    13681 gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gcccgatctg gacagcactg
    13741 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagca aacgccgatg
    13801 aaacgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag
    13861 tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa
    13921 aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc
    13981 gaaaacgcct tatccgccta caaatgcatg aagattcaac atgttgcaat agattttgta
    14041 ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg gcgtgaacgc
    14101 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    14161 tgaacgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca
    14221 gacctatctt caggtaaccc gcctgatgca gcgtgtccat taccttcatc aacgtctcgt
    14281 aatcgacagt tttatccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    14341 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    14401 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    14461 aagcaggcaa gttaaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    14521 tcagcaggac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    14581 tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt
    14641 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    14701 gcaacaatac ctgcgctgcg acatcaccca gcatcgcttt aaagccgcca atctgacgcg
    14761 cgaatacgtt atagataacg accgccggaa tcgctgccac cagaccgatt gccgttgcta
    14821 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga
    14881 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagcccaacg aacggcgaaa
    14941 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgacct actgcggcga
    15001 cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    15061 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    15121 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct
    15181 gctcgcgctt aaggcgacgc ttctgattaa agaactctac gctcttactg aagaagattg
    15241 cccaggtgac tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    15301 cgtgctgata cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt
    15361 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga
    15421 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    15481 cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    15541 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    15601 cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    15661 tacactctcg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt
    15721 atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    15781 cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca
    15841 ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc
    15901 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    15961 agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc
    16021 gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc
    16081 atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg
    16141 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    16201 tttggcatcg atgtttctat tcaagccgcc accaaatatc tggttgggca ttcagatgcg
    16261 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat
    16321 ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    16381 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga atggctggca
    16441 gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa
    16501 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    16561 ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    16621 tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgcagccatt
    16681 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    16741 gatgtcgacg atctgattgc cgatctggac gccggttttg cgcggattgt ataacattgc
    16801 cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg
    16861 atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc
    16921 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    16981 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    17041 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatatt
    17101 ggctggcgca ttgattgccc agggggttat ggattttctg cctacgattg cgattctgac
    17161 cgccgcagca agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac
    17221 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg
    17281 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    17341 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgtt tccagttttt
    17401 caactggtta agcggattgc tgtgggtcag cgtggtaacc agtttcggct atgccttaag
    17461 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    17521 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata
    17581 ctgtaacgcc tgacgatttt ccctgttccc ggttgctgta ccgggaacgt atttaattcc
    17641 cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    17701 actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct
    17761 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    17821 gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatat
    17881 tagcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt
    17941 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    18001 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    18061 tacgcgcatc cagtggtcgc gccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    18121 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
    18181 cttcgccaat atccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
    18241 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    18301 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    18361 gtccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    18421 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    18481 tcagtttatt aactttatcc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta
    18541 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    18601 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    18661 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    18721 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaagcgcaa
    18781 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg
    18841 gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    18901 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    18961 aactggttcg cgaacagaaa gtgactttcc tgctgggggt tggcggcggt tctgtactgg
    19021 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    19081 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    19141 tgccagcaac cggttcagaa tccaacgccg gtgcggtgat ctcccgtaaa accacaggcg
    19201 ataagcaggc gttccattca gcccatgttc agccggtatt tgccgtgctc gatccggttt
    19261 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    19321 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    19381 gcattttgct gacgctgatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    19441 atgtgcgcgc caacgtcatg tgggcggcga cgcaggcgct gaacggtttg attggcgctg
    19501 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    19561 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata
    19621 ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg
    19681 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    19741 tgcctaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    19801 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ctggatgtca
    19861 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    19921 catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat
    19981 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    20041 ccagttggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    20101 agcgttagaa gtgggttatc gctcgtttga taccgccgcg gcctataaga atgaagaagg
    20161 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa
    20221 gctgtggaac gacgaccaca atcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    20281 ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    20341 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    20401 cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc
    20461 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa
    20521 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    20581 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat
    20641 tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    20701 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga
    20761 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    20821 acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta
    20881 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    20941 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    21001 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    21061 ttaccgtgca cgcacagccc ccctcacaga ccgtagaaca aacagtccgg catatttatc
    21121 agaactataa atcagatgcc actgcccctt attttggtga aaccggagag cgtgcgataa
    21181 cttctgtgcg tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggtaata
    21241 ttggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgctagaaag
    21301 cgtagcgata accgaaactg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21361 taaagatgat aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg
    21421 ggtcattgac gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21481 tgaaaaaacg ctggccgctt tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc
    21541 cgaactcttt gaacatattg ccgattatag ctggccgtgg acgtgggtgg tttccgactc
    21601 ttaccgccag gcggtcaatg ccttctataa aaccaccttc aagacggcca acaatcccga
    21661 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21721 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21781 cattcatatt cgttttacgc tgaccaatgg caacaacgaa gagcaagaac tggttttaca
    21841 acggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    21901 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaatagcaa
    21961 ttagccggaa caataaaaaa gggaacacta tatgaaaacg attttcaccg tgggagctgt
    22021 tgttctggca acctgcgtgc tcagtggctg cgtcaatgag caaaaggtca atcagctggc
    22081 gagcaatgtg caaacattaa atgccaaaat cgcccggctt gagcaggata tgaaagcact
    22141 acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc gtcttgatgc
    22201 acaggactat tttgattgcc tacgctgctt gcgtatgtac gcagaatgat aaaaaaatcc
    22261 ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct ttagggcgtt
    22321 tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag
    22381 gcgtctgaca ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt
    22441 tctggcgacg agcctcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac
    22501 ggctgccaat cagatgcttt ttgcccatcg cttccagcgc ctggcggatt aacggccagt
    22561 ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgtttg tcgcccttcg
    22621 gtacgaagac gtcttcactc ttataaccaa tcttcgccag cgggtttttc ccggtgtaat
    22681 acatggtggt cgagttagcc agtggcgatg ggtagaagtt ctgtacctgg tcgagacgga
    22741 aacgatgctt tttcagccac agcgccaaat tcaccatatc ttcatcacgc gtaccggggt
    22801 gcgcggagat gaaatagggg atcagatact gctctttacc tgcctgtttc gagtaggtat
    22861 cgaacagctc tttaaagcgg tcataactgc ccatgcccgg cttcatcatc ttcgacaacg
    22921 gcccttcttc ggtatgttcc ggagcaatct tcagataacc gccgacgtga tgggtcgcca
    22981 gttctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct gaggcaatca
    23041 gaatcttttt aatgcctttc agatcgcgag cgcggcgata gaggttgatc gtcggttcgt
    23101 ggttagtgtc catatgcgga caaatatccg ggtaaacgca ggacaaacga cggcaggttt
    23161 gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat
    23221 cggaaattac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga
    23281 tcgaatcttc ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag
    23341 agcagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg
    23401 gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc gcaaaaacgc
    23461 tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca tagcggtcac
    23521 cgtgtttttg catcaatgcg cgggcgcaac ctgggttagt ttcgtggtgc agaatacgcg
    23581 aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt
    23641 aggttttttc ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct
    23701 ttttcggtgc caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga
    23761 ttgggtcgat ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca
    23821 gcgcctcttt cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc
    23881 ccatcgccag acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca
    23941 tgtcggcttt cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat
    24001 gcgcggtacg gcgcagacta gcctcaatac caccgaggat caccggtaca tctttccacg
    24061 cctctttaca acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt
    24121 tatccggcgt gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca
    24181 tcgaatccat gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcatga
    24241 aatcgtcttt gctgctccag tctggctggg cgatgatccc gacgcgaaag ccctgtgctt
    24301 ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag
    24361 taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca cgagacatcg
    24421 gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc
    24481 gatccggttg gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat
    24541 caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc
    24601 ctcctcgggc gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc
    24661 acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg
    24721 ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc
    24781 tctgtcttcc gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc
    24841 aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg
    24901 cgcggtgaca tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc
    24961 cagactgata tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg
    25021 catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag
    25081 ggtagaaacc agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc
    25141 cgcttcgcca caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg
    25201 gcgctcgccc ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg
    25261 atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat agcgacgaga
    25321 gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct
    25381 ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta
    25441 ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac
    25501 accataatgg ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc
    25561 ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag
    25621 agtagctgcg ttctggcgaa taggcagtac gggtgcccag tcagcgtttg gcgacatcat
    25681 ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac
    25741 attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat
    25801 agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa
    25861 tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagcag
    25921 cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc
    25981 tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc
    26041 tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gtttcccgca aaaatacaga
    26101 ttgccgactt aaacttttcc ggcggcttcg cgctctgcga cttctttatc gagctcggcg
    26161 attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca
    26221 tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga
    26281 ttaagattaa tcttattcga agttgtggag acgcacacgg gaataatcgg gacgcccgcc
    26341 gcaattgccg cgtgaaatgc cccagtcttg aacggtagca ggccgcgacc acggctgcgg
    26401 gtaccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt cactacttcc
    26461 gcaatagtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac
    26521 cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc
    26581 ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata
    26641 tagatagcat tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg
    26701 aacagcggcg caagacgacc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc
    26761 gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta
    26821 ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga ctggcattgt
    26881 acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
    26941 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg
    27001 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa
    27061 cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt
    27121 cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt
    27181 cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa
    27241 tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc cagaccatcc tcgccacgcg
    27301 cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca
    27361 gatcacttac cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg
    27421 aagcatcttc aattaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag
    27481 ccttacctgc acggttacgc gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg
    27541 catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg
    27601 ccccaggcgg caacgttaat ttgccggtga gcggttcgcc ctgaccacgc gccgacggca
    27661 gcgtaatcgg atcaatggca tagctacgac cggtggaatc aacaaacact accggttggt
    27721 tgctcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg
    27781 cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga
    27841 caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca
    27901 acggcgaacg acgatcgtca ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca
    27961 ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca
    28021 gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca
    28081 gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt
    28141 tcggttcatc ttcattacga atgatctcaa tcacttcgtc gatattgaga aacgccacca
    28201 gcaaaccttc gaggatatgc aggcgcttga ggactttctc cagacgatag ttcagtcggc
    28261 ggcgcacggt atcgcggcgg aacaccagcc attcggagag aatttccagc aggtttttca
    28321 ccgccggacg accatccaga ccgatcatat taaggttaat gcgatagctc ttttccagat
    28381 cggtggtagc gaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca
    28441 caatcaccag acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg
    28501 gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct
    28561 gatgcggcaa tgcgctgata accaccgcgc catcttcttt cttccacacc gcgcgcatac
    28621 gcactgaacc acgtccgttc tcgtagattt tacggatctc ggcgcgcgaa gtgataattt
    28681 ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtggttt
    28741 tcggctggtc gattaatgcg attgccgcct gagccacttc acgcaggtta tgcggtggaa
    28801 tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac
    28861 gggcaggtag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg
    28921 tcccctgccc caactcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt
    28981 aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc ccagttcccc tgaccatcaa
    29041 ccagcggata acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcgc
    29101 tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa
    29161 atttggcgct ggcattcagg cccagttcag acatcgcata cacaatgcgg cgctgaacag
    29221 gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt
    29281 ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta
    29341 attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac
    29401 attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg
    29461 acggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga cataatcctg
    29521 gggattatgc agcggaaaac cgccaacgta gggtttaagc agtttgatta acggctgata
    29581 gtagatagga atcagcggtg cctgctggtt gacgatcact tctgcctgct gatacaacgc
    29641 attacgcttt gtcgcatcag tgatctgcat ggcctggttt agtaaggcgt catactgcgc
    29701 atttttccag tgaccgacgt tttcttcact atcgcttttg agcgtgttca ggaagctgga
    29761 agcatcattg tacgtcgcat cccacgactg ccgagacagc atgaaatcac cggctcgtcg
    29821 ggcatcaaga taggttttcc actccattgt gcgcagcgtc acctgtgcac ccagccattt
    29881 tttccattcg gaagacaacg ctatcgcggt cttttcatgc agatcgtact tgttgtagaa
    29941 cagctcaaag cgtagcggat gagaggcgtc gtatcccgcc tgtttcagca aggcttttgc
    30001 catcgcgacg cgctcactca ttggcttttg cagttcatcg aacgtcgtcg cgctaaagcc
    30061 ttttacctct ggcggcgtca gcgtggttgc gggcgttctc aaccccagta ccttttgcgc
    30121 aataagctgt cgatcaaccg taagatatag cgcccgacgc actcgcacat cgttaaatgg
    30181 cggtttctca aggttgaagt tgtaatattc gctgttcaga cgcggaataa ttcgtatctc
    30241 gccaggcagt gatttttcaa tggcgggaat ttgctgcgcc ggaacccagg tgagatcgac
    30301 ctctcccgcg cgatagcggt tatagccggt gaccgaatta tccagcgcca gatactcaac
    30361 ctgttgcaat actgtatgtt gcgcatcgcg gtactttgga tttttgcgtg cagtaatctt
    30421 ttcgttaact acccactgat caagcacaaa ggcaccgttg taaaccatgt tctctggctt
    30481 actccagcta tcgccatgtt tagcgatgac atgatgagga accgggaaca gcgtcggcca
    30541 ggcgagcatc gtcgtgaacc aaggaaccgg ctgctcaagc gtaacttcaa gagtacgatc
    30601 atccgtcgct ttgacaccca gcgatgtaac atctgcttta cccgcaacaa tagccgcggc
    30661 attgttaatg tgcgcctgtg ccagatagcc agcaaaaggg cttgccgttt tcgggtcaac
    30721 cgcgcgctgc cagccgagga caaaatcctc tgccgtcaga ggctgaccgt cggaccactg
    30781 caaaccgcta cgcagatgga aaatatagcg cctgccgccg tccagtatct cccagcgttc
    30841 agcctgagcg ggctgcacct ggccttcacc gtccatccat accagacctt caaacagatc
    30901 cagcacaatc tgcgcggcag tattctcctc caccttttgc gggtcgagcg tacctgggtc
    30961 gctatgattg ttgtaacgaa agacttgttg cggggcgagc tgtgtgttgg tgggaacgtc
    31021 agcagcgtag agaggagccg cacttaccag gctgaccagc cataataaat ttcgcgtata
    31081 catggtattc cttaccggag aaattataag cccaacaaca atacgcctgt ggcattgttc
    31141 gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc atcacgctaa
    31201 cggcacataa acattcgttt ccagtcgctg ctccgtcacc ccttcggcaa gattggtgta
    31261 gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat
    31321 aatgccccat atcgtgtgtg aaatatcgtc cagctcgcca acgtggcgag ccacggcata
    31381 acgtccaccg gtaagttcac cattgctcac accataacga ttatcgggaa tcggttcgct
    31441 aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag ggtcgtccca
    31501 ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt tacgccacat
    31561 gataaatttc gccacgctgt agttgagcaa ctcaggccta cagcggtgcg gcaacatcgc
    31621 cacagggatt gatgggaaat cgataatgtt tacatccagt gtcaggtttg tcatgttgcc
    31681 tccctgcgaa attattcccg ggaacgcatc ccgggagcgg taacaattac ggatttactt
    31741 tgcggatctg tttgacgtca atttcaacag aattccagtc tttatcgact tcaccctgaa
    31801 tctcaaccgt atctttcggc gtcaccgtca cgccgttcca gcgtttgtgg tcgatatcaa
    31861 cattgatagt accgctggca tctttgaaca cgtagagatc gtcagagatg cgttcaacga
    31921 tattgccgcg cagggttacc caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg
    31981 ttacgctgcc gttcggcccc tggaatcctc cggcctgact ttgcgttgcc gatgggccag
    32041 aaaaaccgcc ctgctctgct gccatcaccg gtgcgctgca cagggccatt actgcgatta
    32101 ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga gcatttaaca
    32161 agatagtcct taacaacttc ttaagggaaa aaaataaaat ttagtgctgt acagagcgcg
    32221 ttacaacacg gtttactagc agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt
    32281 tactgataga agatgacatg ctgattggcg acggcatcaa aacgggcctt agtaaaatgg
    32341 gttttagcgt cgactggttt acacaaggtc gtcagggaaa agaggcgctt tatagcgcac
    32401 cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc gatattttgc
    32461 gcgaatggcg agaaaaaggt cagcgtgagc cggtactgat cctgaccgcg cgcgatgcgc
    32521 tggaggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg
    32581 cgttgataga agtcgccgcc aggctggaag ctctgatgcg ccgaaccaac ggccaggcca
    32641 gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg
    32701 ctggcgaacc cttaacactg aaaccaaaag aatttgccct gctggaatta ctgatgcgta
    32761 acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc tgggacgaag
    32821 aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg
    32881 attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga aatttaccca
    32941 acgtcttagt ctgcgtgtca ggctgacgct aatcttttta attctggcct cggtgacctg
    33001 gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg aattgttcga
    33061 cacccaactg atgctgtttg ccaagcggtt aagtacgctc gatctcaacg aaatcaacgc
    33121 ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg atgacgatgc
    33181 gctgaccttt gccatcttta cccacgacgg cagaatggtc cttaacgatg gcgataacgg
    33241 agaagatatt ccctatagct atcaacggga aggttttgct gacgggcaac tggtgggcga
    33301 taaagatcaa tggcgttttg tctggatgac ctcacctgat ggcaaatatc gcatcgttgt
    33361 tggtcaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg ggcaattgat
    33421 cccgtggttg gtcgcactgc cggttatgtt aatcatcatg atggtactac tgggtcgtga
    33481 actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgaccctg actcggaaaa
    33541 accactaaac gcgactggcg tacccagcga agtgcgacca ctggttgagt cgctaaatca
    33601 actgttcgcc cgcacacatg cgatgatggt tcgtgaacga cgctttacct ccgacgcagc
    33661 tcacgaactt cgtagcccgt taacggcgct gaaagtgcaa accgaagttg cgcagctctc
    33721 tgacgatgat ccgcaggcgc ggaaaaaagc actgctccaa ttacattccg ggatcgatcg
    33781 cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac tggataacct
    33841 tcaggacgtc gcggagatcc cgcttgaaga tctcctgcaa tcgtcggtga tggatattta
    33901 ccacacggcg cagcaggcga aaattgacgt gcgactgaca ctcaatgtcc agggcatcaa
    33961 acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg ataacgccgt
    34021 gcgttacagt ccacagggca gcgtggtgga cgtcacgctg aatgccgata atttcatcgt
    34081 aagggataac ggccccggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    34141 tcgcccaccc ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat
    34201 cgccaagctg catggcatga atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc
    34261 gaaggtaagc tggtaagggt aagacttttg ctaaattcgc aaaagacttt gcacattttg
    34321 ctaatttcac cgtaccgctc tgtgacgtac tatagtcggc aaacgtctca ccttgaggtt
    34381 aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc
    34441 aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg
    34501 ttcgcatcgt tcgcgccgac agcgactacg atgtcaaagc ggaagtacaa aactttctct
    34561 gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga
    34621 aaaaaataca ttgatgatgt attcaccgaa ggtcacggga ctctgtatgc cagcgatggt
    34681 cgtacccgca aagatccgtc gaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    34741 tatatgcttt ctctgacctg gaacgcgcca atggaagcct tcaccgaaaa agatcagttc
    34801 ttccacggcg ttggcgttga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctc
    34861 ggtatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    34921 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    34981 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    35041 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    35101 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    35161 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg
    35221 catctgcaaa ccccgcacat gaaggcgtat agcgaagccg taaaaggtga cgtgctggag
    35281 atgaatattc gtattctgca gccagggatt taacattcct gaaaagaggc cggtgttgct
    35341 accggcctca ggtcagatta gccattcttt cttcccgttt tcagcccccg atggaaatcg
    35401 ttaattcgct taatggtttt aaccgcacgc tgcggcaccg cggaagccac cgaccagaca
    35461 atcatttcca ggcacaacaa taccgtacca tgtagtggcg ttttcccctt ttcatccccg
    35521 cgcggaacat gaatcacgat actggcgtct ttgctaaaac gtgaatccag cgcgtttgtc
    35581 agcaaaataa cagggatccc cagccgtctc gcttcacgca gcgttgtcag cccttcccgg
    35641 tgcgcagatt tttgcgccat catgatcaga acatcgccac gttgaagcgc aatcagttgc
    35701 tcggcaaggc caatacccgt acggtttagt gccgtggcag gcaaacctat gcggttgaat
    35761 agtcgtgcgg tatagtccgc cagaataccc gaagcgccaa tgccaaaaat tgcgacttgc
    35821 cgtgcctgta ccaacaacgc taccgcttgc gcaagcgcgt ggcggttttc aggctctgac
    35881 aggacattac aggtatacaa atgcccttca agaacaaagt cgattgcggt gttaacatcg
    35941 ctcgtcaagt tactcaccgt cgtagacatc ttttcgctgg aactaagggc tggaccaagc
    36001 cactgttcca gagtgcgttt cagatcacgc agcccggcaa accccagggc ctgtatagcc
    36061 ctgataactg tggcatcgga agtgttcaac gtggccgcaa tttccatcgc cgtttgatcg
    36121 agtaccactt cacgatgctc attaatatag ctggcgacag ctcttaaccc aggcgttaac
    36181 tgactcgcgc gagtgcgaaa acgctcacca taaatatcaa cccgcgtttt gtctttctga
    36241 atcatttggc ctccggtaaa ggtcgccccg caatttgtga ttgtatctgc gcagccatca
    36301 gccccagaga tgcaaaggag ttagaaattg cctcttcacg agaaatcagc acataacgcc
    36361 cggaacgaca ggcagtcaga aactgacacc atcccggcat gactttttcc attgctgcca
    36421 gctcatcctg aggtttacca ccggtgtcac cgcgccaggt tgcaaaaaca aaatcggcat
    36481 ccagttcagg taaacgctcc gcgctcacat ccattcgccc accttcagga atgctctcaa
    36541 tcagcggcgg aaagcgaaaa ccagcatcgc gtaatacccg ccccagcgaa tgatagctat
    36601 gcatcacatt aatttttccc tgatttgcct gaatcaccga taccgttatt ttttggctgt
    36661 ccagcgtcgc cttcagcgca ttgatttgtg cctgatagcg gcgttcaagt atcgccagct
    36721 gcgactgcgt tcctgtcagt tctgccagct tgcgatagat ttctggcgcg ccccctttga
    36781 gatgatcaat gctcaccgtg ggcgcaattt tttccagtcg ttcaatgggt gtattacggg
    36841 tcggttctgt gatgatcagg tcaggctttg ccgccacaat cgcttcaata tcaatatcag
    36901 cggtgccaat aaaagcgatc gatgaattat caaagtcgac acctgtcaat aacgcgcctg
    36961 atcgaatgaa atggctaccg tcaggccgag ttcgcccgtg gctggcgaca ggaggtacac
    37021 cgagctcaat cagaggaata gtgatatcaa gatcgtgtaa agagacaatt ctctttggat
    37081 gagcaggaac agtgactttg cgccctaaat catcagtaaa gacctgaaca ggttctgtcg
    37141 cgcgggcaaa gaatgacaac agtatcagca gagaaaaaaa taaacgcata taactcccat
    37201 taaaaactgt tcctgcgctg ccagagcagt agcaaaaaga atggtccacc aatcaatgaa
    37261 ataatgattc cggcaggcag ttgcaaaggc aaaaaagcca aacgaccaat gctatccgcc
    37321 aggatgacta acaacgcccc cgatacggcg ctccccagca gtaatgtcgt ctgcccgcca
    37381 cgtagaataa aacgtgatat atggggtgct atcaatccga caaaaccgat gttaccaaca
    37441 cacgaaacgc agacggctgt caggattatt ggtgcggcga cacgtaacaa cgccagacga
    37501 gaactattca cccctaaccc ggttgcaacc tgatgaccaa gcagagcgat atccgccgtc
    37561 ctggcggtga acagcaataa aagagcggct ggcaacatcc aacaggctga aataccgact
    37621 aacatccagt tagcagcatg caggcttccc gataaccaca tcaatgcagt ttgtacatct
    37681 cgaacatctg cagtggtcat aaatacaccg ataccggcgg cgaaaaacca ggaaacacca
    37741 ataccaatca gaacaaatcg cgggcgggaa atttcgcgtg cacaaaaaat cacgattaaa
    37801 gccaccaaca agccaccagc aagaccagcc actggacgcc agaacattcc cagcattgga
    37861 aactggaaaa tcagccacag caccgccacg ctacatcctt ctttgacgcc gattaaacca
    37921 ggatcggcaa ggccgttacg cgcaatggat tgcatcgcgg cccccgccat acctaacatg
    37981 gctccgcata gcaatgccat gatgactctc ggcaagcgaa tatcctgcac aatataacgt
    38041 gcgtctgtgc ttacgttttc tggagcaaac aacgctcgtc ctacttccga tgccgggaca
    38101 aaaaaagaac catgcatcag accgaatatc agtattccaa ccgcaaacag aaacagccca
    38161 ccaagcaaca ccagattttt agggcgtacc agtgtcgaaa acttaccaac acgtagtgga
    38221 cgtagcccga cgttcattat ccgactcatt tgaacattct cgtcgccata atgacaaaaa
    38281 caggcgcacc aactaacgcc gtcatgacac cggtcgctaa ctcatggggc gtgaaaagcg
    38341 tccgagcaat aatatctgcc agtaataaca aaagcgcgcc gacacaagct gaaagaggga
    38401 gcagaacgcg caaatccgct gataccagac gcctgacaat ctgcggtact agcagaccga
    38461 caaaaccaat ggggccagca atcgatacgg ctgcgccaca gagcaacgcg atcgcaagca
    38521 gagtgaatgt tcgcgtgcgg agtaccgaga cgcccagccc ctgcgccatg cgatcaccca
    38581 gtgccaacat gttaagagag ggcgcaaggt aaatggctaa cacaaccccc cccagagaaa
    38641 accaggcgga cgtacccagc gtcgcccaat cctgccccgc taaatcacct gctagccatg
    38701 tccgcatcgc cagcagtgtt tgttcgtcaa gaatcaggat tgcagccgtt attgaggaca
    38761 cgaacgccga tagcgccacg ccgcataaag taactttcat tggcgttagc cctgaacgcc
    38821 ctgcggagga aagcagcaat atcaacagga acaataacgc gccgcccgta gatgccagca
    38881 gtgggcgtcc aaccggaaag gccagcccaa gcgcactggc agcaacgaca gcaagagcgg
    38941 ctccggcatt taagcctaaa atatgtggct cccccaatgg gttacgaatg acagcctgca
    39001 acaaagcacc agcaactcca agagaagcac cggtaagcaa tgctgccgct aaacgcggca
    39061 aacggagtct tacaattacg ttgtgatcga agttttgcgg ttcaaaatga agaaatgcct
    39121 gcaacaccgt ttgtggtgca ataaatcggg caccaatccc tggatatacc acgcatccca
    39181 caagcagtaa ggcgaggaaa attacagtaa ccagccctgt gcgcatcata ctttttctac
    39241 accacgaaac ggcatgaaga acggctttcc cgtcagagta tttattgatg cgtgaacatc
    39301 aacgtcaaag acagctttta ccagctcagg cgtgcaatgc tccccctcat tcaacacacg
    39361 caccactttc ccctggcgta aaaatatcaa cgtatcacca taattgacgg caaaattcag
    39421 atcatgcaac accacgacga ctgtacgccc gtgatgacgc gttaaatcat gtaataactc
    39481 aagaatttca acctgataac gtaaatcaag ccaggttgtg ggttcatcca gtaggatata
    39541 aggcgtcttt tgcgccaaca ccatcgcaat ccagcaacgc tggcgctgcc caccagagag
    39601 tttttcaaca ggtaaatgag caaactcctg cgtaccggtt aatttcaggg cctcttctac
    39661 cgcctgctcg tcggcgtcgc tccattgacg tatgaaattt tgccagggaa aacgcccacg
    39721 cgacaccagt tcataaacgg ttaagccctc cggcaataat ggcgactgcg gcaagatccc
    39781 aagtctacgg gataacgctt tcgttggttg ctcgtgaatc gctttaccat ccagcaatat
    39841 tgagccgccc atgggttgca agacacgtgc aatggtgctg agcaacgtcg actttccaca
    39901 gccgtttgcc ccaaccagaa cggtcatttt ttctgtggga acggagaaag aaacaccatc
    39961 aacaatgatt ttcttgtgat acccggcaga aacattgtcg aggatcagcc cctgcccttt
    40021 tgcgttatgt gccacgtgta cgccaactta atca
//
LOCUS       DAZKFZ010000019        38957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000019 DAZKFZ010000000
VERSION     DAZKFZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            55..2571
                     /locus_tag="ACHOQW_000941"
     CDS             55..2571
                     /locus_tag="ACHOQW_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572057.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            2647..3102
                     /locus_tag="ACHOQW_000942"
     CDS             2647..3102
                     /locus_tag="ACHOQW_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFP9572058.1"
                     /translation="MIHLFKTGMITAFILGVTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            3181..3414
                     /locus_tag="ACHOQW_000943"
     CDS             3181..3414
                     /locus_tag="ACHOQW_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9572059.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            3514..4332
                     /locus_tag="ACHOQW_000944"
     CDS             3514..4332
                     /locus_tag="ACHOQW_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9572060.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHFPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            4633..5106
                     /locus_tag="ACHOQW_000945"
     CDS             4633..5106
                     /locus_tag="ACHOQW_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9572061.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDGTWVLFNAMNGN
                     RAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            5122..5598
                     /locus_tag="ACHOQW_000946"
     CDS             5122..5598
                     /locus_tag="ACHOQW_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9572062.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVSPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            5667..5888
                     /locus_tag="ACHOQW_000947"
     CDS             5667..5888
                     /locus_tag="ACHOQW_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9572063.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWYGGTEAYTSREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
     gene            6051..6425
                     /locus_tag="ACHOQW_000948"
     CDS             6051..6425
                     /locus_tag="ACHOQW_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9572064.1"
                     /translation="MSDALPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRADIRGRFSDADSYHLEQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            6472..6849
                     /locus_tag="ACHOQW_000949"
     CDS             6472..6849
                     /locus_tag="ACHOQW_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9572065.1"
                     /translation="MNTLPDTHVRETSGCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNDITLGKHPEAKQ"
     gene            6846..7334
                     /locus_tag="ACHOQW_000950"
     CDS             6846..7334
                     /locus_tag="ACHOQW_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9572066.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDIDGIELAELINVARDNDYS
                     LRVVDESDRTSADCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            7346..7543
                     /locus_tag="ACHOQW_000951"
     CDS             7346..7543
                     /locus_tag="ACHOQW_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9572067.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLQQNR"
     gene            7628..8476
                     /locus_tag="ACHOQW_000952"
     CDS             7628..8476
                     /locus_tag="ACHOQW_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9572068.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYHSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKVCYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIAKHYAG
                     KVSSSV"
     gene            complement(8535..8738)
                     /locus_tag="ACHOQW_000953"
     CDS             complement(8535..8738)
                     /locus_tag="ACHOQW_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572069.1"
                     /translation="MLSKQIPLGIYEKALPAGDELNQAILLNNKYIFKHLPINSPQYS
                     PQGVVVIHGGILNSVEIFSVMVQ"
     gene            complement(8748..9398)
                     /gene="ulaD"
                     /locus_tag="ACHOQW_000954"
     CDS             complement(8748..9398)
                     /gene="ulaD"
                     /locus_tag="ACHOQW_000954"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9572070.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(9412..9876)
                     /gene="ulaC"
                     /locus_tag="ACHOQW_000955"
     CDS             complement(9412..9876)
                     /gene="ulaC"
                     /locus_tag="ACHOQW_000955"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP9572071.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(9886..10191)
                     /gene="ulaB"
                     /locus_tag="ACHOQW_000956"
     CDS             complement(9886..10191)
                     /gene="ulaB"
                     /locus_tag="ACHOQW_000956"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP9572072.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(10207..11604)
                     /gene="ulaA"
                     /locus_tag="ACHOQW_000957"
     CDS             complement(10207..11604)
                     /gene="ulaA"
                     /locus_tag="ACHOQW_000957"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9572073.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11959..13023
                     /gene="ulaG"
                     /locus_tag="ACHOQW_000958"
                     /pseudo
     CDS             11959..13023
                     /gene="ulaG"
                     /locus_tag="ACHOQW_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
     gene            13131..13886
                     /gene="ulaR"
                     /locus_tag="ACHOQW_000959"
     CDS             13131..13886
                     /gene="ulaR"
                     /locus_tag="ACHOQW_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP9572074.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(13883..14632)
                     /gene="yjfP"
                     /locus_tag="ACHOQW_000960"
     CDS             complement(13883..14632)
                     /gene="yjfP"
                     /locus_tag="ACHOQW_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9572075.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARLLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            14814..15143
                     /gene="bsmA"
                     /locus_tag="ACHOQW_000961"
     CDS             14814..15143
                     /gene="bsmA"
                     /locus_tag="ACHOQW_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP9572076.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            15292..15567
                     /gene="yjfN"
                     /locus_tag="ACHOQW_000962"
     CDS             15292..15567
                     /gene="yjfN"
                     /locus_tag="ACHOQW_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP9572077.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(15684..17309)
                     /gene="aidB"
                     /locus_tag="ACHOQW_000963"
     CDS             complement(15684..17309)
                     /gene="aidB"
                     /locus_tag="ACHOQW_000963"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP9572078.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(17393..18556)
                     /gene="yjfC"
                     /locus_tag="ACHOQW_000964"
     CDS             complement(17393..18556)
                     /gene="yjfC"
                     /locus_tag="ACHOQW_000964"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9572079.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(18559..19197)
                     /gene="yjfM"
                     /locus_tag="ACHOQW_000965"
     CDS             complement(18559..19197)
                     /gene="yjfM"
                     /locus_tag="ACHOQW_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP9572080.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(19207..19605)
                     /gene="yjfL"
                     /locus_tag="ACHOQW_000966"
     CDS             complement(19207..19605)
                     /gene="yjfL"
                     /locus_tag="ACHOQW_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP9572081.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(19623..20282)
                     /gene="yjfK"
                     /locus_tag="ACHOQW_000967"
     CDS             complement(19623..20282)
                     /gene="yjfK"
                     /locus_tag="ACHOQW_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP9572082.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(20333..21031)
                     /gene="yjfJ"
                     /locus_tag="ACHOQW_000968"
     CDS             complement(20333..21031)
                     /gene="yjfJ"
                     /locus_tag="ACHOQW_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP9572083.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(21050..21451)
                     /gene="yjfI"
                     /locus_tag="ACHOQW_000969"
     CDS             complement(21050..21451)
                     /gene="yjfI"
                     /locus_tag="ACHOQW_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP9572084.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(21578..22309)
                     /gene="rlmB"
                     /locus_tag="ACHOQW_000970"
     CDS             complement(21578..22309)
                     /gene="rlmB"
                     /locus_tag="ACHOQW_000970"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9572085.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(22490..24931)
                     /gene="rnr"
                     /locus_tag="ACHOQW_000971"
     CDS             complement(22490..24931)
                     /gene="rnr"
                     /locus_tag="ACHOQW_000971"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9572086.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(24970..25395)
                     /gene="nsrR"
                     /locus_tag="ACHOQW_000972"
     CDS             complement(24970..25395)
                     /gene="nsrR"
                     /locus_tag="ACHOQW_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP9572087.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(25600..26898)
                     /gene="purA"
                     /locus_tag="ACHOQW_000973"
     CDS             complement(25600..26898)
                     /gene="purA"
                     /locus_tag="ACHOQW_000973"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9572088.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(27002..27199)
                     /gene="yjeT"
                     /locus_tag="ACHOQW_000974"
     CDS             complement(27002..27199)
                     /gene="yjeT"
                     /locus_tag="ACHOQW_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP9572089.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(27281..28285)
                     /gene="hflC"
                     /locus_tag="ACHOQW_000975"
     CDS             complement(27281..28285)
                     /gene="hflC"
                     /locus_tag="ACHOQW_000975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP9572090.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(28288..29547)
                     /gene="hflK"
                     /locus_tag="ACHOQW_000976"
     CDS             complement(28288..29547)
                     /gene="hflK"
                     /locus_tag="ACHOQW_000976"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP9572091.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(29633..30913)
                     /gene="hflX"
                     /locus_tag="ACHOQW_000977"
     CDS             complement(29633..30913)
                     /gene="hflX"
                     /locus_tag="ACHOQW_000977"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP9572092.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(30990..31298)
                     /gene="hfq"
                     /locus_tag="ACHOQW_000978"
     CDS             complement(30990..31298)
                     /gene="hfq"
                     /locus_tag="ACHOQW_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP9572093.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(31384..32334)
                     /gene="miaA"
                     /locus_tag="ACHOQW_000979"
     CDS             complement(31384..32334)
                     /gene="miaA"
                     /locus_tag="ACHOQW_000979"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9572094.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(32327..34174)
                     /gene="mutL"
                     /locus_tag="ACHOQW_000980"
     CDS             complement(32327..34174)
                     /gene="mutL"
                     /locus_tag="ACHOQW_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9572095.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(34184..35521)
                     /gene="amiB"
                     /locus_tag="ACHOQW_000981"
     CDS             complement(34184..35521)
                     /gene="amiB"
                     /locus_tag="ACHOQW_000981"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP9572096.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(35540..36001)
                     /gene="tsaE"
                     /locus_tag="ACHOQW_000982"
     CDS             complement(35540..36001)
                     /gene="tsaE"
                     /locus_tag="ACHOQW_000982"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9572097.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(35973..37520)
                     /gene="nnr"
                     /locus_tag="ACHOQW_000983"
     CDS             complement(35973..37520)
                     /gene="nnr"
                     /locus_tag="ACHOQW_000983"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP9572098.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            37519..38658
                     /gene="queG"
                     /locus_tag="ACHOQW_000984"
     CDS             37519..38658
                     /gene="queG"
                     /locus_tag="ACHOQW_000984"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9572099.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(38641..38694)
                     /locus_tag="ACHOQW_000985"
     CDS             complement(38641..38694)
                     /locus_tag="ACHOQW_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572100.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         9389 a        10658 c         9712 g         9198 t
ORIGIN      
        1 cagtgccaca ttcactggca ttcacaataa catgatattc atcacggagt gactatgtta
       61 cagatagtcg gcgcgctgat cctgctgatc gcaggatttg ccattcttcg ccttttgttc
      121 agagcattaa ccagcacggc gtctgcgctg gcaggattcg tattgctgtg tctgttcggc
      181 ccggctttac tggctggcta tatcactgaa cgcataaccc ggttattcca tattcgctgg
      241 ctggcaggcg tatttctgac gattgccgga atgatcatca gcttcatgtg gggacttgat
      301 ggtaaacata tcgcactgga ggctcatact tttgactctg taaaatttat tctgaccacc
      361 gctctcgccg ctggtctgct ggctcttccc gtgcagataa gaagcattca gcagaacggg
      421 ctcacacctg aagatatcag caaggaaatt aacgggtatt actgctgttt ttatactgct
      481 tttttcctta tggcgtgttc tgcatacgca ccattgatcg cattgcagtt cgatatttca
      541 ccctcactga tgtggtgggg cgggttgttg tactggctgg ctgcattagt gacgctgcta
      601 tgggcggcca gccagatcca ggcgctgaaa agactgacca gtgccatccg ccagacactg
      661 gaagaacaac cggtgctcaa cagtaaatcg tggctgagca gtttgcaaaa cgattacagc
      721 cttcctgaaa cgctgacgga gcgcatctgg ctgacactca tttcacaacg gatttcccgg
      781 ggagaactga gggaatttga actggcagac ggaaactggc tactggacaa tgcctggtat
      841 gaaagaaaca tggcgggttt caacgaaaag ctgagagaga gcctgtcatt tacccctgat
      901 gaactgaaaa ccctcttccg gaaccgcctg aatttatcac cggaagcgaa tgacgatttt
      961 ctcgatcgtt gcctggacgg cggtgactgg tacccctttt cagaaggccg ccgttttgta
     1021 tcattccacc acgtggatga gcttcgtatc tgtgcctcct gcgggctgac agaagtacat
     1081 catgccccgg aaaatcataa gccggatccg gaatggtact gctcctctct ttgtcgcgaa
     1141 acagaaacac tgtgtcagga catttatgaa cgttcttaca ccggttttat ttccgatgca
     1201 acggcgaatg gtctgattct catgaaactg ccggaaacct ggagtacaaa tgagaaaatg
     1261 tttgcttccg gagggcaggg acatgggttt gccgctgaac ggggaaacca tattgtcgac
     1321 agagtccgtc tgaaaaacgc acggatcctc ggtgataata atgccagaaa tggagcagac
     1381 agactggtca gcggaacaga aatccagacg aaatattgtt caactgcagc ccgtagcgtc
     1441 ggtgcggcat tcgacggaca aaacggacag tatcgttaca tgggaaatca tggtcccatg
     1501 caactggaag tcccccgtga tcagtatgcc ggcgctgtgg aaaccatgaa gaataagatc
     1561 cgcgaaggta aagtacccgg tgtaaccgat cccgcagaag cgtcccggct gattcgtcgg
     1621 ggacatctga cttataccca ggcccgtaat atcacccggt tcgggaccat cgaatcggtc
     1681 acttatgata ttgccgaggg gtcggttgtc agtctggcgg ccggagggat cagttttgcc
     1741 ctgacggcat cggtcttctg gctcagcact ggcgatcgcg atgctgccct gcagacagct
     1801 gctgtccagg caggaaaaac cttcacccgc acactggctg tctacgtcac aacccagcaa
     1861 cttcaccggc tcagtgttgt tcagggtatg ctgaaacaca ttgatttttc gactgccagc
     1921 ccgactgtcc gactggctct tcagaagggg actggtgcag gaaatatcag tgccctgaac
     1981 aaagtaatga agggtacgct ggtgacatct ctggcactgg tagctgtcac aaccggccct
     2041 gacatgatca aaatgttgcg gggacggatc tccggtgcgc agttcatcag gaatcttgcc
     2101 gtggcatctt ccggtgtggc aggtggtgct gtcgggtcag tggcgggcgg gatattgttc
     2161 agtccactgg gaccatttgg tgcactgaca gggcgtgtgg ttggcggtgt tctgggggga
     2221 atgattgcct ccgctgtatc aggaaaaatt gccggagcgc tggttgaaga agatcgcgtc
     2281 aaaattctgg caatgattca ggagcaggtg acatggcttg ccggcagttt cctgctgacc
     2341 ggacatgaga ttgaaaatct gaacgagaat ctggcccgtg ttatcgatca gaatgctctg
     2401 gagatcattt tcgccgccgg tatacaacaa cgggccgcga ccaatatgtt aatcaaacca
     2461 ctggtggtca gtatcatcag gcaacgcccc gtcatggaat atgatgcatc ccatctcggc
     2521 aatatggtta accgactgga agaagcatta cccccggagt taccggcata acggctattc
     2581 tgtgcctttt acgttttatc acctgatttg tcagtgtcat cgtcatgact cagagaggta
     2641 aataccatga ttcacctgtt caaaaccggc atgattaccg ccttcattct gggggtaacg
     2701 tggtctgccc cactccgggc acaggatcaa cgttacatca gtatacgcaa tacagatacg
     2761 atatggctcc cgggaaatat ttgtgcttac cagttccggc tggacaatgg cggaaacgat
     2821 gaaggatttg gccccctcac catcactctg caactcaaag acaaatatgg tcagacgctg
     2881 gtgaccagaa aaatggaaac ggaagctttt ggtgacagta atgccacgcg aaccacagac
     2941 gcatttctgg aaacggagtg cgtggaaaat gtcgccacaa ccgaaatcat taaagcaact
     3001 gaagaaagta acggccatcg tgtcagtctg ccgttatcgg ttttcgatcc ccaggactac
     3061 catccactgc taattaccgt ttccggaaaa aacgttaact gatccctgaa gtcagtttca
     3121 tgcttcagcc tctgtcacaa cgcgtggcag aggcttttta tttatcagga gaaaacagat
     3181 atgccgggtt acacagagta tgtcctggct gaaggatctt tctcatacgg gcaggcggtt
     3241 gctgtcataa cagcttaccg caatgtgttt atccaggatg acccgggaat gcatttccgt
     3301 cgggttatcc gcaacgcgga aggtcagcga cgctggcgct gccggaattc tgaacccgat
     3361 gccggaaagg tgcttaatac gcggctcgcc agtgacggtc ttctcagaca ataaacgtca
     3421 ttcatttcac ccatcaggcc gcgtcttctc cgggagacgt ggctttttca tttattacac
     3481 cagtaaatct taaccaccga taaggagcaa attatgcgac tagccagtcg ttttggttat
     3541 gcgaaccaga tacgccgtga ccgtccgctg acacacgaag aactgatgca ctatgtgccg
     3601 gggattttcg gggaagataa acacacgtcc cgaagccaga actatacgta catccccacc
     3661 atcaccgtac tggaaagcct gcagcgggaa ggctttcagc cattcttcgc ctgccagacc
     3721 cgtgtgcgcg acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc
     3781 ggagagataa acggagaaca tttccctgaa attattctgc tcaactctca tgacggttca
     3841 tccagctacc agatgttacc cggatatttc cgtgccattt gtaccaatgg cctggtctgc
     3901 ggtcagtctc tgggagaagt gcgtgttccg caccggggaa atgtggtgga cagagtcatt
     3961 gaaggggctt acgaggtggt gggcgtgttt gaccggattg aggaaaagcg tgatgccatg
     4021 cagtcgctga ttctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac
     4081 cgttatggtg acgaacatca gcccgtcacc accgctgaca ttctgacacc gcgacgcagg
     4141 gaggattacg gtaaggacct gtggagtgct tatcagacca tccaggagaa tatgctgaaa
     4201 ggcgggattt ccggtcgcag tgccagagga aaacgtatcc acacccgtgc cattcacagc
     4261 atcgacaccg atattaagct caaccgcgca ttgtgggtga tggcggaaac gctgctggag
     4321 agcctgcgct gatgcagcct gtccctgaag gcgctgccgg gatgtcgttc catttctgat
     4381 gatgacctgt gcgcctggtg tacccgtctg ctttaccgtc ccggagaaat cagtctgtgc
     4441 agactgagta caggcaatgg catctggcca tccgtctgtg accggaacgg ttacgcgtac
     4501 ggctgtccgg gatttcagcc gaatatcatt catccctgat attaccgggt ctcacggtca
     4561 gtgatgaacg tgatgttgcc agctcccgac ctttactccc tctcatttat tcacattaca
     4621 aggatttcat acatgaaaac attgtctcag aacaccacct cttcggcctg tgcaccggag
     4681 actggcctgc aacaactggt tgccaccata gtccctgatg aacagcgcat cagtttctgg
     4741 ccgcagcatt ttggcctcat tccacagtgg gtgaccctgg agccccgtgt cttcggctgg
     4801 atggaccgtc tgtgtgaaga ctactgcggt ggtatctgga atctgtacac cctgaacaac
     4861 ggtggcgcat ttatggcacc cgaaccggat gacgatgatg acggaacatg ggtactgttc
     4921 aatgccatga acggtaaccg cgctgaaatg agtccggaag ctgccggcat tgccgcctgt
     4981 ctgatggcgt acagtcatca cgcctgtcgt acagagtgtt atgccatgac ggtccactat
     5041 taccgtctgc gggattacgc cctgcagcat ccggaatgca gcgccattat gcgcatcatc
     5101 gactgaatgg aggcaggaac aatgcagcag ctttcctttc tacccggaga gatgacgccc
     5161 ggcgagcgca gcctcattca gcgggccctg aaaaccctgg accgtcatct tcatgaacct
     5221 ggtgtggcct tcacctccac ccgtgcggca cgggaatggc tgattcttaa catggcggga
     5281 ctggagcgtg aagaattccg ggtgctgtat ctgaataacc agaatcagct gattgccggt
     5341 gaaaccctct tcaccggcac catcaaccgc acggaagtcc atccccggga agtgattaaa
     5401 cgcgccctgt accacaatgc cgctgctgtg gtgctggcac acaatcaccc gtccggtgaa
     5461 gtctcaccca gcaaggcaga ccgccttatc accgaacgtc tggtacaggc actgggcctg
     5521 gtggatatcc gggtgccgga ccatctgata gtcggtggca gccaggtttt ctcctttgcg
     5581 gaacacggac tgctttaacc cgtcaccgtc acaaccacat cacacctgtt ttcactttta
     5641 tcttctgtct tcagaggtat cacactatga aaatcatcac ccgtggtgaa gccatgcgta
     5701 ttcaccaaca acatcctgca tcccgtcttt ttccgttctg cacaggtaag taccgctggt
     5761 acggtggcac tgaagcgtat accagtcgtg aagtgcagga tattcccggt gtgctggccg
     5821 tgtttgcaga acgccgtaag gacagttttg gctcgtatgt ccggctgatg agcgtcaccc
     5881 tgaactgatg ttccgtcacc cggaaaacga gtacgaccag atggactcag acgccgctgt
     5941 ggatgaggcc ctgctcatca acgaatacac ctttaccgaa gccggtctgc acgccggctg
     6001 acctgtcatc gttaaactat cattcaccga ttttctgtga ggattttatc gtgtcagacg
     6061 cactccccgg gacaacgctt cccgacgaca acaaagaccg cccctggtgg gggctgccct
     6121 gcaccgtgac gccctgtttc ggggcacgtc tggtgcagga gggtaaccgg ttgcattacc
     6181 ttgcagaccg cgccgatatc agaggccgct tcagcgacgc ggattcgtac cacctggagc
     6241 aggcctttcc gctgctgatg aaacaactgg agctcatgct caccagtggt gaactcagcc
     6301 cccgctatca gcataccgtc acgctgtatg cgaaaggact gacctgcgaa gccgataccc
     6361 tgggctcctg tggttacgtt tatctggctg tttatccgac gccggcaccc gcaaccacct
     6421 cataaccccg aaactctcac ctttagcact accagttaca gagaacttaa aatgaataca
     6481 ttacccgaca ctcacgtacg ggagacatcg ggctgcccgt ctccggttga aatctggcag
     6541 acactgctca cccgactgct ggaccagcac tacggcctca cgctgaatga cactccgttc
     6601 gctgatgaac gtgtgattga gcagcatatt gaggccggca tttcactgtg cgatgcagtg
     6661 aactttcttg ttgaaaaata cgcactggtg cgtaccgacc agccggaatt cagtgcctgt
     6721 acccgttctc agttaataaa cagtattgat atcctccggg cacgccgggc aaccggcctg
     6781 atgacccgcg acaactacag aacggtaaat gacattaccc tgggtaagca tccggaggcg
     6841 aaacaatgaa actggccctg acgctggaag ccgacagcat taacgtgcag gcactgaaca
     6901 tggggcgcat tgtcgttgac atcgatggta ttgagcttgc cgaactgatt aacgtggccc
     6961 gcgacaatga ctactccctt cgtgttgttg atgaatctga ccggacctca gcagactgca
     7021 cgccaccatt tgctgccctt accggcatac gctgcagtac cgcacatatc acggaaacgg
     7081 acaacgcctg gctgtactcg ctgtcacacc agactaatga cgacggcgaa acagaatgga
     7141 ttcattttac aggcagcgga tatctgttac gtaccgatgc gtggtcatac ccggttctgc
     7201 ggcttaagcg cctgggcctg tcaaaaacgt tccgtcgtct ggttgtcaca cttacccgac
     7261 gttatggcgt cagtctcatt catctggatg ccagcgctga atgcctgccg ggtttaccca
     7321 ctttcaactg gtaaccagga acaacatgaa atcattaacc acggaaacag cactggatat
     7381 tctgattgcg tggctgcagg acaatatcga ctgcgaatcg ggaattatct ttgacaacga
     7441 tgaggataaa acggattcgg cagcactgct gccctgcatt gaacaggcga gggaggatat
     7501 ccataccctg cgccaggtgc agcttctgca acagaaccgg tgagtcttac tcaccagact
     7561 tcattccact gacgccagcc tgaacagggc tggcgttttc atttatctgc gaaaaggaat
     7621 atcgattatg tctgcaatca cagtctcccg tccggaagtg gtcaccgggc atacggacgt
     7681 tatctgctcc acctcagtca gtcacattct ggctgtgcga aagactacac tgctgcaaat
     7741 cgacacactt atccggcaac tggctgaaat ctcggtactg acagaaagta ttggaggtaa
     7801 aactgctctg gactgggcca tgaaacagga ttttcgctgt ggctgctggc tcatggagaa
     7861 accggaaacc gcaatgaaag ccatcacccg caaccttgac cgcgaaatct ggcgtgacct
     7921 gatgcaacgt tccgggatgc tgtcattaat ggatgcgcag gcccgtgata catggtacca
     7981 ctcactggag tacgataact ttccggaaat cagtgaagcg aacatttgga gcacatttga
     8041 gcaactgcac cagaataagg atgaagtgtt tgagcgagga gtgatcaacg tcttcagagg
     8101 gctgagctgg aattacaaaa ccaattcccc ctgtaaattt ggcagtaaaa ttatcgtcaa
     8161 caacctggtg aggtgggaca gatggggatt gcatcttatc accgggcaac aggcagatcg
     8221 actcgccgac ctggaaagaa tgctacacct gttcagcggc aaaccgatcc ccgacagccg
     8281 ggaaaatatc atcattcggc tggatgatca catccgctcc gttcagggca aagtgtgcta
     8341 tgaagatgag atgttcaaca tcagatactt taagaagggc tccgcacaca tcacgttcag
     8401 gaaaccagaa ctggttgatc ggctaaatga tattattgcg aaacattatg cgggaaaagt
     8461 atcttcttca gtatagactt atatatcaca ggtattgtgt ctcgtccgga acaagacagg
     8521 taggtatgct gcattcactg aaccattaca gagaatattt caacagagtt caatatacct
     8581 ccatgtataa caactacacc ttgggggcta tattgggggc tatttatagg gaggtgttta
     8641 aatatatact tattattcaa taatatagct tgattaagtt catccccggc ggggagcgct
     8701 ttttcataga tgccaagcgg gatttgtttg gacaacatat ccgctcctta gccccacagt
     8761 tcggcgatgg aacgtttgaa ctgacgtgcg gcttccaccg gagaagcggc atcacggata
     8821 ctacggcccg cgataaagac gtgaatcgga atgcctttga acagcggcag atcttccagc
     8881 gccaggccac cagtgacggt gactttgaag cccatatcgg aaagacgttt gatcgcggtg
     8941 atgtccgctt cgccccacgc cacgcctgcg gcctgcgcgt cacggctgcg gtgataaacc
     9001 acctgctgaa tacctgcgtc gcgccactgt tgcgcctgtt cccaggtcca gtaaccggtc
     9061 agttcgatct gcacgtcgcc gttaaactct tttgccacgt ccagcgcgcc tttggcggtg
     9121 ttgatatccg cacagcaaat taccgtcacc cagtcagcgt tggcttcgaa gcacatacgc
     9181 gaaaggattt tgcctgcatc ggcaattttg gcgtctgcca gtacgatttt gtgcgggtag
     9241 agcgctttca ggtcacgaac cgcacgcacg ccttcgccca cgcacagaat ggtgcccact
     9301 tcgataatgt cgacttcttc ggcaatcagg cgagtggttt cgtaggcgct atccatagtc
     9361 tggttgtcca gcgcgacttg caacatcggt aatgacatgt tcaattcctt cttaagctgc
     9421 cgcgttggtg cggtcaatga gatccagtac ttcctgctcg gtacggcagg cgcgtaaacg
     9481 gtcaaaattc tcttcatctt caaacaggtt gacgatctgc atgatgccca cttcctggtg
     9541 agtattggca tcgaccgccg ccatggtgat gaggatatcc accggatcgt tgtcatcgtg
     9601 gttgaactcc agcggctttt tcagcgttac cagagagaaa ccggttttct taacgccctc
     9661 ttccggacgc ccgtgcggca ttgccaggcc cggagcgata acgaaataag gaccgaactg
     9721 ttcaacgcca tccagaatcg cctggtagta acgcggctct accacatctg ccgccaccag
     9781 caggtcaacg ccgattttca ccgcttcctg ccatgtctct gcttcagcct gcagacggat
     9841 ggatttattt tcagccagcg aatcacgtaa tttcatggcg cgtccttact tcacgtcctg
     9901 cgggaaatgc tctttgatca cttccagcag tttcgggcca aagtcggcag gagagagcat
     9961 gttgcgcacg ccaaccacgt atttgttgcc ggtcacggtg atttcgcccg caatgtgcgt
    10021 agaagcgatg atgatatccg cgccactcaa ctcgcttttg tactcgccaa ccgcgcagct
    10081 gtttaccgta tggtcaatgt ttgattgggt taaaaactgg tccactttca tcttcatgat
    10141 catggaactg ccttgtccgt tgccacacac agccagaata cgtacggtca taatcaaaac
    10201 tccttattaa gcagactgtt ctgccagttg tttttctgca tcttcttctg cgcgcagcgc
    10261 gcggccagcg aagaacatat aagccagtgc aattacaatg atgacggcca taaaggcgat
    10321 accgatggag aagaagcctt gcatcatcgg cggtgccaga atcgaccagt ccgccatgcc
    10381 catccaggca ctcataccgg tgagtttcac cgcccagacg caaccaaaga tttcgatcat
    10441 tcccatcacc agacaaatct tcagcgctgc acgccagccg ccgaagtggt tagcgaacac
    10501 gccgatggtg gcgttggaga agaacatcgg gataaagcct agaataatca gaatggaaga
    10561 gccgcaggcg accaggatgc caactgcaat cagctgaccg atggtgcccc acataaagcc
    10621 ccagaccacg gcgttcggcg cgaagctata gatagccgca cagtcaatcg ccagaaccgc
    10681 acctgggatc aggcgctggg agataccgtt aaatgcttca gagagttccg ccacaaacat
    10741 gcgcacgccc tgagtgatga tgaagatcgc caccgcgaag gagaaaccgg tttgcaggat
    10801 gtagaccgtc cagtgcactt tgcccgccat cgcctgcacg gtgtcgatac cgaaggagag
    10861 cagaatggcg ccaaagaaga tggtcatcac aatcgccgtg gagacgatgt tgtcgtggaa
    10921 aatgttcagc cagcccggca gtttgaggtc ttcaacgctc tcctcttttt tgccgaggaa
    10981 cggcgcgact ttataggcaa tccatgatgc aaactgctgc tggtgaccga tggagaaacc
    11041 acagccatcc gtcacttcct gagtcggctt gtacatcatg ttggaagtga tgccccagta
    11101 gagcgaaacc agaatcgcgg tacagataat ggtggtccac atggagtagc cgaagataaa
    11161 cagcgtaacg gcaatcaacc cggcctgctg gaacatgatg tggccggtca acatgattgt
    11221 gcgaatgccg gtaatgcgac gcaacagcac gtaacagatg ttcagtgcca gcgctaacaa
    11281 tacggcgtaa cccacccagc tataggcatc gcccatgcgg tcgatggttg ccatcattga
    11341 agcgtaggta tcggaaattg cgccgttaat gccgtagact tcggacattt tcgccaccac
    11401 cggtttgaag gtgctggtga ggatgccgga ccctgcctgc aacaacatga aaccaattat
    11461 ggttttaatc gtgcctttaa taataacgct gacacttttg cgcagtagga tgtagcccag
    11521 acaggtcaca atacccagca acaacggggc attggtcatg acctggttaa aaaatacggt
    11581 aaagatgttg tagaggatct ccataacgat ctcctgaaga agaggtaacc gggtattcca
    11641 cacacccgga atgttgtgca ctcactctaa ttttcaaaag taatcacaac aagattattt
    11701 atgattaaat gtgacgcgac ccgcaaataa ttccacatgg atagagcggg attcacgcaa
    11761 cgaaacaatt attcatttaa attcatttag ttaattgaat tatacattcc attaaatctt
    11821 tccatactca aaatcaacaa caacttccct ggcaaaaagt caaaaaatgg tcattgcctt
    11881 ccagaaaaaa tagtgtcagg attaatcaaa accaatcaca tattgattcg aattgaacta
    11941 tcatgaaggt aaatggcgat gagtaaagtg aaaagtatca cccgtgaatc ctggatcctg
    12001 agcactttcc cggagtgggg tagctggttg aatgaagaaa ttgaacaaga acaggtcgct
    12061 cctggcacat ttgcgatgtg gtggcttggc tgcaccggga tctggttgaa atcggaaggt
    12121 ggcaccaacg tttgcgttga tttctggtgc ggcactggca aacaaagtca cggtaacccg
    12181 ttaatgaaac agggtcacca gatgcagcgc atggctggcg tgaaaaaact gcagccaaac
    12241 ctgcgtacca ccccgtttgt tcttgatccg tttgcgattc gccagatcga cgcggtactg
    12301 gcgactcacg atcacaacga tcatatcgac gttaacgtcg ctgctgccgt gatgcagaac
    12361 tgtgctgatg acgtaccgtt tatcggaccg aaaacctgtg tggatttgtg gattggctgg
    12421 ggcgtaccga aagagcgttg catcgtggtc aaaccgggcg atgtagtaaa agtgaaagac
    12481 attgaaattc atgcgcttga tgctttcgac cgtactgcac tgatcaccct gcctgccgat
    12541 caaaaagcgg ctggcgtact gccagatggc atggacgatc gcgcggtgaa ctacctgttc
    12601 aaaacgcctg gcggctccct gtatcacagc ggcgactccc actactctaa ctattatgcg
    12661 aagcacggta acgaacatca gatcgacgtg gcgttaggat cgtacggcga aaacccgcgc
    12721 ggtatcaccg acaagatgac cagcgccgat atgctgcgta tgggtgaagc gctgaatgcg
    12781 aaagtggtta tcccgttcca ccacgatatc tggtcaaact tccaggccga tccgcaagag
    12841 atccgcgtgc tgtgggagat gaaaaaagat cgtctgaagt atggcttcaa gccgtttatc
    12901 tggcaggtgg gcggcaaatt tacctagccg ctggataaag acaacttcga gtaccactat
    12961 ccgcgcggtt tcgatgattg cttcactatt gaaccggatc tgccgttcaa gtcattcctg
    13021 taatctgtcg tttataccgg atagcgtgga gaagttcgcg ctatccggcg tagtatttcc
    13081 tggctatttc aaatatcatc taaaaaaatc aaattttatc ggaatagctc atgactgaag
    13141 cacaaagaca tcaaatcctc ctggaaatgc tcgcacaatt gggctttgtg accgttgaga
    13201 aagtcgttga gcgtctggga atttcgcctg ccactgcgcg acgcgatatc aataaacttg
    13261 acgaaagcgg caaactgaaa aaagtgcgca atggtgcaga agctattacc caacagcgcc
    13321 cgcgctggac gccgatgaat ctgcatcagg cgcagaatca cgatgaaaaa gtacgtatcg
    13381 ctaaagcggc ctcgcagctg gttaatccgg gcgaaagcgt agtcatcaac tgcggctcca
    13441 ccgcgtttct gcttgggcgg gaaatgtgtg gcaagccagt gcaaatcatc actaattatc
    13501 taccgctggc aaattacctg atcgatcaag aacatgacag cgtgatcatt atgggcggac
    13561 agtacaacaa aagtcagtcc atcactttaa gcccgcaggg cagcgaaaac agtctctatg
    13621 ccgggcactg gatgtttacc agcggaaaag ggctgaccgc agaagggttg tataaaaccg
    13681 atatgctgac agcaatggca gagcagaaga tgctgagcgt ggtagggaaa ctggtggtac
    13741 tggttgatag cagtaagatt ggcgaacgcg cgggaatgct ttttagccgt gccgatcaaa
    13801 tcgatatgct tatcaccggc aaaaatgcta acccggaaat cctgcaacaa ctggaagcgc
    13861 aaggggtcag cattctgcgt gtttaaagat gctggcggaa aaatgtcacg gcagcatcta
    13921 acgcctcagg cgtaatgcgg tgacgcacgc ctggctgcca tgaacaggtt agcagtttat
    13981 cccgtcccgt ctcgcttaag gcctgctgca aacgtagtga ttcgtcggca ggcacaacgt
    14041 catcgtcgag gccataccac agcagtagag gtctgtcact aagttgttcc aggtggtttg
    14101 tcgcttccca ctctgccagt ggcgcgacaa tgttattgaa ttcattctgc tgtgctaccg
    14161 tttcaggtat cagcggtgga aacagtaaac gggcgagtga tgtaaaatag cccgatccca
    14221 tcatgctggc ggtacatctc accgtggggt ggcgagcggt aatccccagt gccgtcatcg
    14281 cgcccatcga cgcaccaccg actgccagac ggtcatcaag cagccacttt tcttcggcta
    14341 ttgccgcacg taaagtagtg aattcctgca tactttgtag caagatttgc cagaattgat
    14401 ttaaccgccg cgctgcgtca ccactaaaac ggctaccgtg atcgggcgca tccggcatga
    14461 tcacccgcaa accagcctgc gccagcgcaa cggcaaaata gctatacacc agactggacg
    14521 aagtaaagcc gtgataaaaa atcacgcacg gtaacggggt atctttttgt ccgacaggat
    14581 aagcatgaag aacgggaata tctgccagct cgcgtgattc tatttcaatc atctgtctct
    14641 cctttttgtc tggtatgaca tgaagaaatc cttagcacaa gtttctgctg gcggtattag
    14701 ttcataatgt tgagatgtgg gttacgcttt cgtcacgttt tcattcgaaa atcgcgtacc
    14761 aggtaacaat tcgggaacat tcccccaaag ttaaattaat aaggcactac actatggtta
    14821 gcaggaaacg taatagcgtt atttaccggt ttgccagttt attattggtg ttgatgttaa
    14881 gtgcctgtag cgcactgcaa ggtacgccac agccagcacc gccagtaacg gaccatccgc
    14941 aagagattcg ccgcgaccag acgcaagggt tacagcgaat aggtagcgta agcacaatgg
    15001 ttcggggttc tccggatgac gcattagcag aaatcagagc aaaagctgtc gctgcaaaag
    15061 ctgattatta cgttgtcgtc atggtggacg aaaccatcgt gacaggacag tggtattcac
    15121 aagccatttt gtaccgtaaa taaatcagac aacttacata gtcagcttta cattgctttg
    15181 cgtccctatg cgtttacctg tgcacaataa attaccggct ggaagcctgg gcgtttatta
    15241 ccgcgatgga atgccctgca acatgtgggg aaacgaaaaa tggagctgac gatgaaacaa
    15301 ttacttgcct caccctcgct gcaattagtg acttatcctg cgagcgccac ggcacagtct
    15361 gcagaattcg ccagtgctga ctgcgtgacg ggcctgaatg aaataggtca gatctcagtt
    15421 agcaatattt caggggatcc gcaggacgtg gaacgtatcg tggcgttaaa ggccgatgaa
    15481 caaggtgcgt catggtaccg cattattacc atgtacgaag accagcagcc tgataactgg
    15541 cgcgtacagg cgatactgta tgcatgagtg tcatctacag caaatgaaca atgccggatg
    15601 cggcgcgagc gccttgtccg gcctacaatt ttcacatatt tcatttagtt aatcgaaacc
    15661 agcgtcgcat cagtcgtata cgcttacaca cacactcccc ccgtcgcccg cagcaataaa
    15721 tcattctgga tctgctctga caaccgtacg ccgccgcgcg tatctaacat cacctgacac
    15781 cacgcctgcg ccattggcgg agaagcatat ttcaacattt gcgcaccgca gcccagcagg
    15841 aatagctgat gagtaatctc tcgccccagt tcttcagctg gcttacgcag ctgctgctgt
    15901 aaacgacgaa ccgcgcaatc aaaatagcga tcctgccctt tcacttctac aaatgcttcc
    15961 gacaataagt cgtatacgcc cgcttgctta ttgagaacgc gcagcacatc caggcacata
    16021 atattgccgg aaccttccca aatactgttt accggcatct cccggtaaag ccgcggcagc
    16081 tcgctctcct cgcaataacc aatgccgccc agcacctcca tcgcttcggc cacaaacggc
    16141 atacctcgtt tgcagatcac aaattttgcc gcaggcgtaa ataaacgcgc ccacagagct
    16201 tctttggcat cggcgcgccg gtcccacgct cgcgcaagac gaaacagcaa cgccgtttgc
    16261 ccttcaagct gaagtgccat gcgacttaag acatgacgca taaggggctg ttggatcaat
    16321 ggattaccaa aaacatggcg ttgatgcgca tgataaatcg ccagcgagaa cgcacggcgc
    16381 atcatggcat ggctacccag ggcgcaatca aaacgcgtca tcccccccat tttcaggatc
    16441 agacgaattc cttccccttc ctgccccaat aaccagccaa tggcatcctg aaactccact
    16501 tcacaactgg cgttagagcg attacccagc ttatctttca gccgctcgag gcgaatcgcg
    16561 ttgcgttgcc cgtcaggcaa aaagcgcggc acaaaaaagc aggacaaacc gcccgcggtc
    16621 tgcgccagca ccagatgcgc atcgctttgc ggaactgaga aaaaccattt atgccccacc
    16681 agccgataag agccatcctc cagacgctct gcgcgggtgg tgttgctcat gacatcggaa
    16741 ccgccctgct tttccgtcat tcccatgcca atcaacaaac cgcgtttttg cccacctggc
    16801 aataagtgag aatcgtagcg atcgctcaac agcggtgtgg tccagtcctg aaacggcgcg
    16861 ggtaacatct gtaacaacaa tggcgtggcg gcaaaggtca tggttatcgg acataacgac
    16921 cccgcctcaa cctgtgcgtg caacataaaa cgcgccgcgc gcgccacaaa tgcgccggag
    16981 cgagcgtctt cttcccaggc aagattgtgc acccgattgg tacatagtgc ctgcatcagc
    17041 aggtgccagg cggggtgaaa acgcacatcg tccagacggc gtccttgcgc atcgtagcgc
    17101 aatagttcag gcggattcac attcgccagc cgccccagtt caagggattc agccgttcct
    17161 aactgctgac caatactggc tagaaaatcg ctatcccagc cagcaccttc acgcgttacc
    17221 gcttcgcaaa gcgcgccatc agacaggtat aagttgctgt tatttaatgg tataggttga
    17281 ttaaaaacgg tgtgagtttg ccagtgcact gtgtctccct ccatcaatgg cagtcaccat
    17341 tagtatggtc actgccatgg attcatgaca gaaagagatt gctaaaacat tcttatccag
    17401 caatgtaatg cggaacgaaa cgtgaggtgt ctttggtgat cagtgtgtta tcttcacgga
    17461 tccccattcc gcacgcttca tcatcgacaa tccagctacc gatgagtgtg tagctatcgc
    17521 caaaccgcgg cagaggttga aacgcctggt agatcatcgg ttcatcggcg taatcaccat
    17581 cagcgtggtc aacgacgtta ttctggccgt cgaaaatggt gacatttccg ccttcgcgcg
    17641 agtagatcgg tttacgcaca tagctttcgc cagcggcaat ctgcggtttt tcaccctcaa
    17701 accaggatgc aagaaggtta ggatgaccag ggaagaagcg ccaaagcagc ggcattagcc
    17761 ctttattact caagatactt ttccataacg gctccaccca ttgctcgcga cgcttgcgca
    17821 gcagcggacc gttatcgtca cgcatcatcc actccagcgg atacagctta aatgcacgct
    17881 ggatgacatt atcatcaaga tcggtcagta cgccgccgac gcccaaaccg agatcttcaa
    17941 tgtagataaa ccgcgactcc tgccctgcct gctgggcgca gtcctgcaag tacagcacgg
    18001 tactcctgtc ttcatcggtg tcctgacagc agcaaaaata aaacggttcc cgactgtaaa
    18061 gctcgctaaa gcgtgaaatc aggcgttcct gaatagcatt gtactgatcg gcatcacgcg
    18121 gaataatgcc gctgcgccgg gcatcctcca gccacagcca ctggaaataa gccgactcgt
    18181 acaatgaagt tggcgtatcg gcgttgtact ccagcagctt caccggcgca ttgccacacc
    18241 aggcaaaatc catccgtcca tacagcgaag gatcgcgggc gcgccagctt tcagcgatca
    18301 catcccagta caacggcggg atcgccagtt gcgtcaggat ctcttcatct tttaccgcgc
    18361 gatccaccac ctcaaggcac atctgatgca gttccgcagt cggtttttcg atctgctctt
    18421 caatctggcg cagagtaaaa cggtaagccc gactctcatc ccaatagatt tcattgtcga
    18481 tgatatgaaa gtcgaaaccg ttgtcagcgg cgatctggtc cagatcccgt cgcacaggaa
    18541 cgttgtgtct cagcatgatt agcctcccca atgcccacgg gcgctggaag aacgaccata
    18601 gccgccgcga gaaacggttg acgctttttt ggtggtaaag ccgcctgaag agtaagactc
    18661 ttttttgcca gaaccggagc gccaggagta atcaccggaa gtgttgcgcc agacggggcg
    18721 ggaagcaaat gaggatccgc cgctgttata gacaaacggc tcatcgcgat ctttacgaat
    18781 aacccggctt aacaaaaatc cagaaacgac cgggatccaa ctctgttcaa cattgtcgta
    18841 gtagcaattt tcgtacttag actgacagtt ctgctgagtc atattcttcg gaacatcggc
    18901 ataaaatgcc gctttggcgt tgttccagcc acgcgcgcaa atgtcggcat tattaccgtc
    18961 atcaatacaa tcctgcactg tcgaataaaa cgttccgtcg ccgtcgttat cgacatcgcc
    19021 actatcgcca caacctttca atacaaaaaa agcggcaccg cccattagcg ccaacgttaa
    19081 gtatttttgt gcgtagcgat tgcgacgcgg ttcaaaagga ttattcggtc taccaatgcg
    19141 actgatcgct ccgtgaccaa ttttactgtt atttcttgat ttgcgttttc tggccatgat
    19201 cgtccattac catgtcatac aagctgcgtt aaaaataccg ccagccagcg cggcggttcc
    19261 cataaacatt cctgctgcgg tattgtgatt aataattttt tcgcttaatg cgggcatata
    19321 aagtctgaca ccagcaaaaa cgagtaactg aatcaccagt gcaatcccgc cccaggcgaa
    19381 atagtctgga atactcaccg cattgattgc cgcactggat aaggggatca cgtaacccaa
    19441 caatgtaccg ctgaacgcca gtgacgctgc ggtattattg tttttgatta attgccattc
    19501 attgtgcggt gtaattttgg agtagataaa cagaaaaata atcaccatag ccacgccaat
    19561 aaaaaaatag gcactaaagg caagaagtga atccagtata tgcattgtaa acgtccttgt
    19621 aattaaccaa taatatgaag tgatgtcagc gggatatcga cacccaacgc gcgcgaaaat
    19681 aaccactctg gctcgccgag atcgttaaaa gattcctcac catttagcag caaatattcg
    19741 taagtatctt cggtcacttg ccgctggtag cccatggtaa aattatggac ttcccattta
    19801 gcatgatttt gattttctac tttttccagc atgtagacgg gttcgatatt tcccggttct
    19861 tcgctgttaa aaaatcgctg ccagcgtttt tcctgccagt ttaaggtcat tgcccccatc
    19921 gttttggcgt tgatcgcctc gcgccagtga ttctctttac tgataccgta gctctcttca
    19981 tagacaaaga gcttgatatc atcaatgtca tcaatatctt cgccgccggt ggtattgatt
    20041 tgcagaaact catcgcctga agtgtagtag cggaaaatct gactaccgcc gcccagatcg
    20101 atgtggctga cagcgactac cgtaaattcc tcacccggca gcgcaatcag caattcatct
    20161 tccagcaaac gaaacgctaa cgtgtcgagc gtaaagccac tattgagatg aagtcccagc
    20221 ggaccgcgag cgatagcagg cttattatcc ttgccaaaca gacgctggaa aaaaccagac
    20281 atattttatc cttaaaatag ttgccagcct tgcagtgagg ctggcaaaaa aattactcgc
    20341 cctgttggcg ttgcagtctt gctaatacat cctgggcgct acttttattg ctaccgccaa
    20401 ttccggcttc cgccagcttt tcgtcaaggt cgcgaccgtc tgcgactttt tctaactgtg
    20461 cggcagcatc caggcgagcc tgacgttcgg cctgacgcgt ttgcaggcgt tttaaggatt
    20521 ctgctgctgt cgaaacgctg gaagatgcgc caacggtaga agtcgttaca gcctgttgtg
    20581 cacgctgcat ggcttcagtg gctttaacga cttccatttg ctgctcaaac tgagcgatac
    20641 gctgcgctgt cgctgcaact gctttttcca cgccatcacg ggagacttcc agattcgaca
    20701 acacttgctc ttcagcggta atgagattct caaggcgcgc gatttcttca gcaacttcgt
    20761 taatcaacga cggattaacg ttcttgctta acgcttccag cgtacgggct tccagactgg
    20821 ctttgcgctc acgtaaatct ttcagcttat cgtgacttaa tttcacccgc gccagcagat
    20881 caacgcgaga ttttccggct ttatcgagtt cagctttagc gtcacgaata tgctgttcca
    20941 gcatgcgcac accctgggtt tcttcaatgg attcttccgc ctgggagata aacgatttcc
    21001 ccagcgtaaa taaacttttt aaaattccca taaatacccg tccttgtaat tagtgtgaat
    21061 attcttctgt aatttcagcc agatccaatg cgttatctac cagcgaggtt atctcaagca
    21121 ggatatcttc aagagaagat ttaagcgata acgccccgaa aacaatgtaa tactcttctt
    21181 gttgtacgct ggagattcct accgatgaca acggcatcat cttctgattt cttaataaga
    21241 aggtattaaa ttcatctgga ttgctgatac tactcaccgg acaaataaag gtttcgataa
    21301 tcatttggcg ggaagtaaaa agaatattga tttgcaaatc gccataatca ttcattttaa
    21361 taattaatgc gttctcgcta tttgttacat caatattttg ttcaggtaca gtttgcagcg
    21421 ccgtcgccag cgccaacgga ttccatgtca tatattattc catatagatt aagtttaaat
    21481 attaataaaa tgaatatttg caatacgtaa ttatcttacc agctatagac aaaaaaaaac
    21541 catccaaatc tggatggctt ttcataattc agaggaacta gctgcgctga cgaaccgctt
    21601 caaataagca aattccggtt gcaaccgaaa cgttcaggga agaaacactt cctgccattg
    21661 ggatgctgat caactcatcg caatgttcac gggtcaggcg acgcatacct tcaccttccg
    21721 cgcccatcac cagcgccagg cgtccggtca ttttgctttg atagagcgta tgatccgcct
    21781 cacctgccgt accgacgatc cagatattct cttcctgcaa catacgcatg gtgcgcgcaa
    21841 ggttagtcac ccgaatcagt ggaacgcttt ctgccgcgcc gcaggctact tttttcgccg
    21901 tggcgttgag ctgtgcggag cgatctttcg gcacaatcac cgcgtgaacg ccagcagcgt
    21961 ccgcgctacg caggcacgcg ccgaggttgt gcggatcggt tacaccgtcg aggatcagca
    22021 ggaacggttg atcgagcgaa gcgatcagat ccggcagatc gttttcctgg tactgacgtc
    22081 ctggcttcac gcgggcgata atgccctgat gcacggcacc gtcgcttttc tcgtcgagat
    22141 attggcggtt tgccaactgg ataaccacgc cctgggactc aagggcgtgg atcagcggta
    22201 acagacgttt atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt tcaggggcgc
    22261 gctccagcag ggcctgcact gcatggatgc cgtaaatcat ttcgctcatt aatgtactcg
    22321 ttgtttacgt tttgcctgat gcactacgtt tatccggtct acatgatccc tgcaatatat
    22381 tgaatttgcg tgattatgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa
    22441 gcgcattttg tcagcaatct aaccctcttc tgtttgaaga gggtaatgat cactctgcca
    22501 cttttttctt cgccgcacgc ttcgctttgg tcgctgcagc tattttctgc gttttcgccg
    22561 atggcttttt cgcttttctc gcgtctttct tcgctgcttt cggcttcgtt tttttctcac
    22621 cgcggaaggc gctgtctggc tcaaagttta cctttttacc gacctgacga cgcttgccgc
    22681 cttttttacc tgcatcgcct tttttcgctt tctcgcgcgc cgttttaccg acgttgcgcg
    22741 gtgcgcgttc gctggagatc aggctaaagt cgattttgcg ctcgtccata ttaaccgctt
    22801 cgacgcgaac ttccacgcga tcgcccaggc gataagtctg gccgctggat tcacccatca
    22861 ggcgttgccc tacctggtca aagcgatagt agtcattgtc cagcgaagag acatggacca
    22921 gcccatcaat gaacaagtcg tccagacgga cgaagaagcc aaagccagtg acgctggaaa
    22981 ttacgccttt aaagacgtta cctacctggt cgagcatgaa gtcacacttc agccagtcag
    23041 acacatcgcg cgttgcttcg tcggcacgac gttccgccat cgaacagtgc tgacccaatt
    23101 gcagcatctc ttccatcgaa taatggtagc caccggtttc agtggtgttg ccctgatgcc
    23161 cctgctcttt cgccagcaga tacttaatgg cgcggtgcag catcaggtca ggataacgac
    23221 gaatcggcga agtaaagtgc gcataggact gcaatgccag gccaaagtga ccacggtttt
    23281 ccggatcgta aatcgcctgt ttcatcgagc gcagcagcat ggtttgcagc atttctgcat
    23341 caggacggtc ggcaaccgac tccagcagct ccgcgtagtc gcgcggttcc ggcttgttac
    23401 cgcccggcag ttccagcccc agctctgcca gcactgaacg gaaagaggta atcgcttcgg
    23461 tgctcggctt gtcgtgaata cggaacagtg ccggttcttt cgctttctca acgaaacgcg
    23521 ccgccgagat attcgccaga atcatgcact cttcaattaa cttgtgtgcg tcgttacgct
    23581 gagtctgttc gatacgttca atacggcgtt cagcgttgaa aatgaacttc gcttcttcgc
    23641 tctcaaatga gatcccgccg cgttcttcac gggctttatc cagcacttta tagaggttat
    23701 gcaactcttc gagatgctta accagcgggg cgtactgctc gcgcagatcc tgatcgccct
    23761 gcagaatatg ccagactttg gtgtaggtca gacgcgcgtg agagctcatc accgcttcgt
    23821 agaatttgta gccagtcagg cggcctttcg acgaaaccgt catctcgcac accatacaca
    23881 ggcggtctac ctgcggattg agcgagcaca gaccgttaga gagcacttcc ggcagcatcg
    23941 ggataacctg cgaagggaag tacaccgacg taccacggtt acgcgcttct ctgtccagcg
    24001 gcgttggcgg acgcacatag tagctgacgt cagcaatcgc gacccataaa cgccagccgc
    24061 cgccgcgttt tttctcgcag taaaccgcat cgtcaaagtc acgggcgtct tcgccatcaa
    24121 tggtgaccag cggtaaatcg cgcaaatcga cacggcccgc ttttgcttct tccggcactt
    24181 cttctttcag cccagcaacc tgttgctcaa cagcctgcgg ccagatgtac ggaatttcat
    24241 gggtacgcag agcgatatca accgccatgc ccgtgcccat attgtcgccc agcacttcga
    24301 cgattttacc caccgctttg gtacggcgag tcgggcgttg ggtcagttca acaaccacca
    24361 caaagcccat ccgcgcgccc atgatctgat cgggcgggat taagatatcg aagctcagac
    24421 ggctatcgtc aggaaccaca aagccgacgc ccgcttcggt aaagtagcga ccaacaatct
    24481 ggctggtttt tggcaccagt acgcggacaa tacgcgcttc acgacgacct ttacggtcag
    24541 cacccagcgg ctgagccagc acctgatcgc catgaatgca ggttttcatc tgctcgctgg
    24601 agagatacaa atcatcttta cgcccttcaa cccgcagaaa gccgtagcca tcacggtggc
    24661 caataacggt acctttcacc aggtcgaggc gttccggcag cgcatagcac tgacgacgag
    24721 taaagaccag ttgaccatcg cgctccatcg cgcgcaggcg gcgacgcagg ccttcaagct
    24781 gctcttcgcc ttcaatgtgc agttctaccg ccagctcatc acggctggcc ggtttttcac
    24841 gtttggttaa atgttcgagg ataaattccc gactagggat gggattcgcg tatttttcag
    24901 cttcgcgttc ctggaaagga tcttgtgaca tctcggttcc tccgttgtca tctctgatga
    24961 agattttcgt cactccacca gcaataattt ataaagcggt tgattctctt caaccaaatc
    25021 ggcaagcgtg tagttatcca gttccgtaag aaaactttgc acggccttag aaagcgcctg
    25081 tttcaaccga caggcaggtg taatgtggca aaactcactg ctgcaattca ccagcgataa
    25141 gggctccagc tcgcgcacca catcaccaat acgtatcgca ctcgccggtt tacccaggcg
    25201 aatgccgcca ttttttccac gaacagcagt cacgtagccg gcacgactaa gttgattgat
    25261 tattttgacc atatgattac gggagacgcc gtagacgtca gtcacttcag aaatactggt
    25321 catccgccct tctggcaatg acgccatgta gatcagcgca cgtaatccgt aatcagtgaa
    25381 actcgttaac tgcacatcaa cctcaaaagg gaaatcggga aaaaatacat ttatattgat
    25441 gataaaccag ccacaagctg tgtcgctaat ttatttcagt tcggggaagg aaaaaagcga
    25501 ggatttaaca ctgtgtcgga tagcgtaatg gcttaaccga cacagcaatg tatcacactg
    25561 ttagcccggc aggcaaaata tctgccaggc gtaccagaat tacgcgtcga acgggtcgcg
    25621 cagaatcatg gtttcagtac gatccggacc ggtagagatg atatcgatcg gtacaccggt
    25681 cagctcttca atgcgcttga tgtagttcag cgccgcctgc ggcaggccgc tacgatcttt
    25741 cacgccgaag gtggattcag accagcccgg catggtttcg taaatcggct ctacaccttt
    25801 ccagtcgtca gctgccagcg gagtggtagt cacttcacga ccatccggca tacggtaagc
    25861 cacacagagt ttcacctctt tcaggccatc cagaacgtcc agtttagtca ggcagaagcc
    25921 agacagggag ttcagctgta ccgcacgacg aacggcaacg gtgtccagcc agccggtacg
    25981 acgacgacga cccgtagttg cgccgaattc gttaccctgc ttgcagagga actcgccagt
    26041 ttcatcaaac agttcagtcg ggaacggacc tgcacccaca cgagtggagt aagctttgag
    26101 gatacccaga acgtaatcaa cataacgcgg gcccaggccg gaaccggtcg ccacgccacc
    26161 agcagtggtg ttggaagaag ttacgtacgg ataagtaccg tggtcgatat ccagcagcgt
    26221 accctgcgca ccttcgaaca tgacgaaatc gccacgctga cgcgcctggt cgagcaggtc
    26281 agaaacgtca accaccatag aagtcaggat gtcggcaaca gccatcgtat catccagaac
    26341 tttctggtaa tcaaccgctt cagctttgta gtagttaacc aactggaagt tgtgatattc
    26401 catcacttct ttcagttttt cagcgaaggt ttctttgtcg aaaaggtcgc caacacgcag
    26461 accgcgacgt gccactttat cttcataagc aggcccgata ccacgaccgg tggtgccgat
    26521 cgctttcgcg ccacgcgctt tctcacgcgc gttatccagc gcaacatgat aatcaaggat
    26581 cagcggacat gcttcagaca gcagcagacg ctcacgaacg gggatgccac ggtcttccag
    26641 ttctttcatc tctttcatca gcgcggccgg agacagcaca acaccgttac cgatgatgct
    26701 agttacattc tcgcggagaa tacctgatgg aataagatgg agaacggttt tttcaccgtt
    26761 gattacgaga gtatggcctg cgttgtgacc gccctggtag cgtacaacat atttagcccg
    26821 ttcagtcaga agatcgacga tcttaccttt accttcgtca ccccattggg tgcccagtac
    26881 gacgacgttg ttacccattt ttcaaaatca ccgtttgctt aaaaatggat tctaccatcg
    26941 ctttttcaga gttacagcac tttttgcact caaaaatgac ctcgatcagt ctgttttttg
    27001 ttcagccaat cgttttcctc aacatgtagt agaccacaac gcccgcaacc acaagtccac
    27061 cgccaaaacg acgtaaaata ttatcgggca aattggtcat cgcagagatc atcttcttcc
    27121 atgccttcgg gtaaagcatc ggccctaaac cttccagtac caaaaccagg gcaagcgcca
    27181 gccagattgt cgaattcatt cattatcctt atagaaaaag aaaaccaccg acatccctga
    27241 ggatgcggtg gctttattga cctgtaccgc agtcgttata ttaacgcgtt gcggaagtcg
    27301 gcgtcttcat gtagcggaag aaatcgctat ccgggctcat gaccatcacg tcctgattgc
    27361 cagagaagct gttctcataa gcacgcaggc tacggatgaa tgcgtagaag tccggatctt
    27421 tactgaatgc atcagcaaac agtttggctg cttcggcatc gccttcaccg cgcatgatgc
    27481 ggccctgacg ctcagcttct gccagcgttc tggtcacttc atagtcggca gtcgcgcgca
    27541 gtttttccgc ttcttcctga ccttgtgaac ggtgacgacg cgctaccgct tcacgctcgg
    27601 cgcgcatacg gttgtagatc gcttcagaca cttcggtcgg caggttgatc tgcttaattc
    27661 gcacatcgac aacttcaata cccagcgccg ccatactgtt cgggttgatg accggaactt
    27721 tgcccttcgt ctcagccgtt acgcgctctg ccgcttcggc aatggcgtta tctgccgccg
    27781 gggtagtaac ttcatcttct gtacccgcag aaccggagtt cagcgcgtca cgtacttcga
    27841 gggtcagacg accacgggaa tcggtgacga tgtctttcac gtccaggcga ccaatttcag
    27901 aacgcagacg gtcagagaac ttacgtttca acagcacttc cgcttgcgaa atgtcgcccc
    27961 cccccgttgc caggtagtaa cggctgaaat cgctgatgcg ccatttgatg taagagtcga
    28021 cgatcaggtc tttcttctct ttggtcacaa agcggtcggc ctggttgtcc atggtctgaa
    28081 tacgtgcgtc gagcattttc accgtttcaa tgaacggtat cttgaaatgc agacccggct
    28141 cataaaccag aggtttgttg tcatcgtcac gcagtacctt accaaaacgc agcgtaatac
    28201 cgcgctcacc ttctttgacg acaaagacag acatgtaaag cactaccagc acgatgatga
    28261 taatcgcgat aactgactta cgcatcgtta ttccccctga cgctggtagt cgttacgctg
    28321 cgcattggcg cggcgttggt ccataatatc gccctgactg gtggacgacg tgttgcttgc
    28381 tccactggtt gtggaggaag aggctggcgg cagacgcagc agattgctgg caccgttatc
    28441 gctcttcgcc gcaggggcgt taccaccttt cagcatctgg tctaacggca gaaccatcag
    28501 gttgccacct ttatcgttaa ccagcacttt gcgggtgtta cccaacactt tttccatcgt
    28561 ctcgatatac agacgctcgc gagtaatttc cggcgcggct ttatattccg gcagaagttt
    28621 agcaaaacgc gccacttcac cctgagcttc caggatggtc tgggccttgt acgcacgcgc
    28681 ctcttcgagg atacgttgcg cctgaccgtt cgcacgcggc tgaacttcgt tggtatacgc
    28741 ttctgcttca cgaatgtatt gctgttcgtt ttcacgcgcg gcaatcgcat cgtcaaacgc
    28801 cgcttttact tcttccggcg gacgagcagc ctggaagttg acgtccagca gcgtgatacc
    28861 catgtcatac ggacgaatcg tctcttccag ttcgcgctga gtatcgctac gaatcacggt
    28921 acgaccttcc gtcagaatgc ggtccatggt gtatttaccg ataactccac gcagggcgct
    28981 gtcggtagcc tgacgcaggc tgtcatccgg gctggtcacg ctatacagat atttttccgg
    29041 attggtgacg cggtactgca cgttcatctc aacgcgcact acgttctcgt ccgacgtcag
    29101 catcacacca gaagctgcca gttcacgcac ggcttccacg ttcaccggtt tgacttcgtc
    29161 gataaacgtc ggtttccagt tcagacctgg ctcaaccaga tggctgaatt taccaaagcg
    29221 tgttaccacg ccgcgttcgg cttctttaat ggtatagaaa ccactggccg cccagataat
    29281 gacaatcgct gccgctgcga tggtaacgac acgaccgcca agctgcgggc gcgggccttg
    29341 cgatgaactg ccaccgccag atccggtgcc tttaccgccg cccagaccac cgagcttttt
    29401 gctcagtttg cggaagatat catctaaatc aggtggccct tgatcgcgac cgcctttgtt
    29461 tccatttccc tcagagttgc cgccaggttt gctgcttccc cacgggtcgc ggtcttgtcc
    29521 gttattaccg ggctgattcc acgccatgtt tgtgctccat atttgttatg cggtgatccc
    29581 tgttgtcttt cgacgccaga ggatatgact ccacgcttca gacgctacgc cgttagatca
    29641 ggtaatcgat caacgccggt tcttgtttac agaggcgacg ccagtcaacg atcggcatac
    29701 gaacttgcag acttacgctg ccgtcctcct ccatccactc tttttctatt gcctgaagct
    29761 gataaaaacg acttctcaga cgcccttcct gcggtggcag acgcaatgta tgctgcgcca
    29821 cctcgccgga aagccgctcc gtcaaagcct gaaaaagctg tggtatcccc gctccggtct
    29881 gtgcggaaag ccagacacgg atcggtttgt tctcttcgtc ccgatcaata cgcggttcga
    29941 aatcttccag catatcgatc ttgttcatca ccagcagggt tgggatctcg tgagcgtcga
    30001 tctcttcaag aaccgtattc accgcttcga tgttttcttg tacacgcaca tccgccgcat
    30061 caatcacatg cagcagcaat gtggcttgcc gcgtctcttg taacgtggct ttaaatgctg
    30121 ccaccagatc gtgcggcagg tggcgaataa accctacggt atctgcaagt acggtttcac
    30181 cgacatctgc aacgtcaata cgccgcaacg tcgggtcgag ggtggcaaac aactggtctg
    30241 ccgcgtagac ccgcgcttcg gtgatgcgat tgaaaagggt agatttaccg gcgttggtat
    30301 atcccaccag cgaaacagta ggaacgtcgg ctttgatacg cgattgccgc ccctgctcac
    30361 gctgcttttc aactctttcc aggcgcgact gtatctgcac gatgcgatta cgcaacaaac
    30421 gacggtcggt ttcgagctgg gtttcacccg gaccacgcaa accaatcccg cctttctgtc
    30481 tttcaaggtg ggtccagcca cgcaccaggc gtgtagccag atggcgcagc tgcgccagct
    30541 caacctgcaa cttaccctca tgggtacgcg cacgttgggc gaaaatatct aaaataaggc
    30601 cggtgcggtc gataacacga cactcgcaca aacgctccag gttacgctct tgcgccgggc
    30661 tcagggcatg gtcaaaaaga acgaccgaag cacccgtcgc tttgacagct tccgcaattt
    30721 caactgcttt accttcacct acaaaatact ttgggtgcgg cgctttacgg ctaccggtaa
    30781 tcacctgcaa tgcttcgaca ccggcggaag agaccagaga ttcaaactcc tggaggtctt
    30841 ccatatcttt gtcttgcgta aaatagatgt gtaccagtac cgcctgctca ccagcatcat
    30901 aacggtcaaa caagcgtata accctctaaa tagatcagcg gggaacgcag gatcgctggc
    30961 tccccgtgta aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg
    31021 gaagtattct gcgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg
    31081 ttactgtgat gagaaaccgg gcgagacggg acaacagtag aaatcgcgtg cttgtaaacc
    31141 atctggctga ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct
    31201 tgcagcttaa taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg
    31261 ttcaggaacg gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt
    31321 atttgtactt tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca
    31381 cattcagcct gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt
    31441 ttcactgtca agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc
    31501 caactgtctc gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgcctcc
    31561 aaggtaagac cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa
    31621 atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg
    31681 aaaacgctgc tcaattcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg
    31741 atgcacctga tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc
    31801 cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct
    31861 tgctgccgca accggatcga tctcctgaag ttgacgatgc aatgactccc aaccttgctc
    31921 tgccgcctgt tgctcaattc tggcccgtac ttccgggtct gccgacggta gcggcgacaa
    31981 cccttccagc aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc
    32041 cgccgcggtg atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta
    32101 agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc
    32161 gttcggcttc gccgtcccga tatccatccc tttgtaaata agggcagaat caacgcttat
    32221 caactctact ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt
    32281 cggccccatc aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca
    32341 gggcttttat cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca
    32401 caagttgcgg acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc
    32461 actgcgcatg ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga
    32521 atacggactg cttcgctagg tagcctatca gttcaggaat caagatttgt aaattttgtt
    32581 ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa
    32641 tacccaattc cgccagggca gactgcgctt tttctaatgc cgatttttct tcgccagaaa
    32701 cttttagccg caacggaatc agcaacggct gggcgcaaac gggcgcttca cccggcgtca
    32761 gttgtacctg acgcagccaa cgttctgcca ctggcaaggc taaaagtgaa atgttgccgt
    32821 cgcgctccag caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac
    32881 tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg
    32941 gcgtttgcaa aagctggcga tacacttcac cttgctgttt ctggtaacct ggctgcgcat
    33001 tcggccaggg ggcagccgga cgactacctg atgctggcgc aggagtgtag cgcggagcta
    33061 ctggctcacg aactgccggt tcagcaaagt gattgcgccc cgccgccacg cggttttccg
    33121 gaatgggacg cggtgcaggt tggggttcat cgtccagcgg tagcggcgtt tccagttgct
    33181 gttgtagcac gctcagcacg ccctgataga taaagtcatg caccagacgc gactgatgaa
    33241 aacgcacttc gtgtttggcg gggtgcacgt tgacgtccac ctgatgcggg tcgatctcca
    33301 gatacaacac aaatgcaggt tgctgatcgg cccccagttt gtcttcacag gcctggcgga
    33361 tcgcgtgatt gatcaggcga tcgcgcatca tgcgaccatt cacgtagcaa tactgaattt
    33421 ccgccagtgc gggcgtggtg tgatttggat cggccaccca gccgcgcagc gtgagatcgc
    33481 cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc ggtgccacaa atcgcgccta
    33541 agcgccgttc tttttgcccg ccttccggca ccgcgcggta ctggcgtacg attttgccgt
    33601 tatgcgacag gttgatcgtg acgtcgaaac gcgccagtgc gatgcggcgg atgatttcat
    33661 cgatatggtt aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt
    33721 agaacaggtc cagtacctcc agcgtcgttc ccaccggatg cgccgccggt ttaaccgtca
    33781 cgtccatatc gcgcccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg
    33841 aagtgagcgt caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca
    33901 ggctgataat ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgcgcca
    33961 gcgccagcgc cagctcgtct tttttgatac cacagccgtt atcacgaatg cggataagtt
    34021 tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt
    34081 ccaccagttc tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt
    34141 tcgccagttg tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc
    34201 gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc accctgcggc gcagattgca
    34261 tcggatgcgc aaggaaataa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt
    34321 ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga
    34381 ccagtactga tgggatatcc ggcgagcgca gaacgccaag gctggcgtgt tctggtcgac
    34441 gtttatgtat ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc
    34501 ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag
    34561 actgactgtt cgccagcaca tcacccgccc cgcccagcag ctccgactgt ttctcgtgct
    34621 gctccagcca gctggccatt tcgctgttgg cgcgacggtt agagagcacc catacggaag
    34681 cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt
    34741 tttgcttacg tgccacatcg ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg
    34801 ttaaaacgcc tttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg
    34861 cgatggtgac atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc
    34921 cgtgtccggc atcaatagcg atgataattt tatcgccatt gttagccgtc gcgcgcgctg
    34981 ccggacgcgt taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa
    35041 acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac cgtaggcgtt tcaacgcgtt
    35101 tcacaaccac aggcggcgga ggaggcggtg gcggcgcatc ggcgttaatc gtaaagacga
    35161 cagtgtaatt gctgccattc tgccgcttca ccgcttcggt tttaccattt tcggtaagat
    35221 cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc ggagcgaatc gccttcacca
    35281 gattattacc gctgaacaac aacggcagtc cctgaatcac gcccgtttgt ttgatatcga
    35341 gcgccacggt gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca
    35401 acgttatccg cgcctgttgg ttgccgttag aaacctgaat atcagagagc gtcgcggcac
    35461 ccacctgcgc gcacagcagc agcaacgtcg ctaccaacca atttctgatg cgatacatca
    35521 tcccaccacc tttcaaaggt taaccggcta aacgtgccag caacaattca cccgcagagg
    35581 aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat
    35641 ccgggtcagg aagaacacct gtaccttgtt gtggccactc caccaggcag atggcatcgt
    35701 tggcaaaata atcgcggatc cccataaact ccagctcctc gggatcggca aggcggtaca
    35761 aatcaaagtg ataaaccatt aaattgtcga gcgtataggg ttcgaccagc gtataagtgg
    35821 ggcttttgac attaccctga tggcccaaag cctgtaaaaa gccccggcta aaggtggttt
    35881 tacctgcgcc taaatcgcca tacagataga ttacggttgc gccatcgcag gctttcgcta
    35941 cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact cgattcatca
    36001 tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt
    36061 gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg
    36121 acacagcctg cacaggctgc atcatacggc gacagttttt gcccaagcaa tgcgccaata
    36181 ataccagaga gcacatcgcc catgccgccg cttgccatgc ctgcatttcc ggcatcaata
    36241 atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg
    36301 ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg
    36361 acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg gcgtgatcac gcgattgtga
    36421 cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg
    36481 cgaaaattct caactttttg cagggctttt ttcccccact cttgctggcc cagaccggga
    36541 ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat agtcagttca
    36601 tgcaccatca attctggtcg tgcagtcagc agcggcgcaa tgttctcact gcgggtcagt
    36661 actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc
    36721 gccgtgccgt gatcgccacc gataatcacc agccgcccgt gatcgccttt atgcgaagtc
    36781 gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc
    36841 tcctgacctg ccagccagct atccagcccc agtgagtcaa aatgcagttg tcctgtaaca
    36901 tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa taaaagtgat ggtgtgatcg
    36961 gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca
    37021 accgccacaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg
    37081 cggggtgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagagc taccgactcg
    37141 ggccagacaa tattcgaagc atggatctcg cctcccgcgt ttaaccatgc ctcgcgtgcc
    37201 agcgcggcct cttccggcaa cggtttatcg ctctcctggg ccaacaacgt gacctcaatg
    37261 ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc cgccgttatt accatgaccg
    37321 cacaacacca gccagtggcg ggcgtcagga tacgccgaac gacacacctg gaatgcggcc
    37381 tcgccagcgc gaagcatcag ctcatagagt gtgagcccca gcgcatctgc cgcctcgcgt
    37441 tctccgcggc ggatatcgtc ggcgtgccag acggtgtgtg gtatacttac ggggtttttc
    37501 ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt
    37561 aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc
    37621 gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg
    37681 atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattgcccgg tacgctgcgc
    37741 gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa
    37801 aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg
    37861 cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt
    37921 agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc
    37981 ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcgtt cttcttttta
    38041 ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggc
    38101 aaatgcgtgg cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc
    38161 gatgctcgcc gctgtatctc ttatctcacc atcgaactgg aaggggcgat cccggaagag
    38221 ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg
    38281 tggaatcgct attcacaact cactacagaa gacgatttca gcccgcgtaa gccgctacac
    38341 gcaccggaac tcattgagtt attcgcctgg agcgaagaga agtttttaaa agtcacggaa
    38401 ggttcggcga ttcgccgtat aggtcacctg cgctggctgc gtaatatcgc cgtggcatta
    38461 ggcaatgcgc cctgggatga aacgattctg gcggcgctcg agagtcgcaa aggtgagcac
    38521 ccacttcttg atgagcacat agcgtgggcg atggcgcagc aaatcgagag gcgaaatgcg
    38581 tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtaat tgaaaaaggg
    38641 ttaccgcgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat
    38701 tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cgaaatctta atttttaaat
    38761 tatctatcaa taggttaaaa aaatactatt caccgtgcga agtttctttg aaattaagat
    38821 tacggcctga ggctgtggat aactctgttt acaagagttt gtcagagaca acaaaaacca
    38881 gcaataacgc atcgcgtcgc tgtggatatt ttattgagag aagaatttgg agcgggaaac
    38941 gagactcgaa ctcgcga
//
LOCUS       DAZKFZ010000020        38020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000020 DAZKFZ010000000
VERSION     DAZKFZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            118..729
                     /gene="ruvA"
                     /locus_tag="ACHOQW_000986"
     CDS             118..729
                     /gene="ruvA"
                     /locus_tag="ACHOQW_000986"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9572101.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            738..1748
                     /gene="ruvB"
                     /locus_tag="ACHOQW_000987"
     CDS             738..1748
                     /gene="ruvB"
                     /locus_tag="ACHOQW_000987"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9572102.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1994..2779)
                     /gene="znuB"
                     /locus_tag="ACHOQW_000988"
     CDS             complement(1994..2779)
                     /gene="znuB"
                     /locus_tag="ACHOQW_000988"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP9572103.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2776..3531)
                     /gene="znuC"
                     /locus_tag="ACHOQW_000989"
     CDS             complement(2776..3531)
                     /gene="znuC"
                     /locus_tag="ACHOQW_000989"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP9572104.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3610..4542
                     /gene="znuA"
                     /locus_tag="ACHOQW_000990"
     CDS             3610..4542
                     /gene="znuA"
                     /locus_tag="ACHOQW_000990"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP9572105.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4558..5880
                     /gene="mepM"
                     /locus_tag="ACHOQW_000991"
     CDS             4558..5880
                     /gene="mepM"
                     /locus_tag="ACHOQW_000991"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP9572106.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6000..6971
                     /gene="lpxM"
                     /locus_tag="ACHOQW_000992"
                     /gene_synonym="msbB"
     CDS             6000..6971
                     /gene="lpxM"
                     /locus_tag="ACHOQW_000992"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9572107.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7102..8544)
                     /gene="pyk"
                     /locus_tag="ACHOQW_000993"
     CDS             complement(7102..8544)
                     /gene="pyk"
                     /locus_tag="ACHOQW_000993"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9572108.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8672..9541)
                     /gene="hexR"
                     /locus_tag="ACHOQW_000994"
                     /gene_synonym="yebK"
     CDS             complement(8672..9541)
                     /gene="hexR"
                     /locus_tag="ACHOQW_000994"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP9572109.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9879..11354
                     /gene="zwf"
                     /locus_tag="ACHOQW_000995"
     CDS             9879..11354
                     /gene="zwf"
                     /locus_tag="ACHOQW_000995"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9572110.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11589..13400
                     /gene="edd"
                     /locus_tag="ACHOQW_000996"
     CDS             11589..13400
                     /gene="edd"
                     /locus_tag="ACHOQW_000996"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP9572111.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            13437..14078
                     /gene="kdgA"
                     /locus_tag="ACHOQW_000997"
                     /gene_synonym="eda"
     CDS             13437..14078
                     /gene="kdgA"
                     /locus_tag="ACHOQW_000997"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9572112.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14134..15312)
                     /gene="purT"
                     /locus_tag="ACHOQW_000998"
     CDS             complement(14134..15312)
                     /gene="purT"
                     /locus_tag="ACHOQW_000998"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP9572113.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            15446..15736
                     /gene="yebG"
                     /locus_tag="ACHOQW_000999"
     CDS             15446..15736
                     /gene="yebG"
                     /locus_tag="ACHOQW_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP9572114.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15803..16159
                     /gene="yebF"
                     /locus_tag="ACHOQW_001000"
     CDS             15803..16159
                     /gene="yebF"
                     /locus_tag="ACHOQW_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP9572115.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16486..17145
                     /gene="yebE"
                     /locus_tag="ACHOQW_001001"
     CDS             16486..17145
                     /gene="yebE"
                     /locus_tag="ACHOQW_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFP9572116.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17354..19414
                     /gene="ptrB"
                     /locus_tag="ACHOQW_001002"
     CDS             17354..19414
                     /gene="ptrB"
                     /locus_tag="ACHOQW_001002"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP9572117.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(19411..20073)
                     /gene="exoX"
                     /locus_tag="ACHOQW_001003"
     CDS             complement(19411..20073)
                     /gene="exoX"
                     /locus_tag="ACHOQW_001003"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP9572118.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20097..20753)
                     /gene="yobB"
                     /locus_tag="ACHOQW_001004"
     CDS             complement(20097..20753)
                     /gene="yobB"
                     /locus_tag="ACHOQW_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP9572119.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20855..21085)
                     /gene="holE"
                     /locus_tag="ACHOQW_001005"
     CDS             complement(20855..21085)
                     /gene="holE"
                     /locus_tag="ACHOQW_001005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP9572120.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21224..21598
                     /gene="yobA"
                     /locus_tag="ACHOQW_001006"
     CDS             21224..21598
                     /gene="yobA"
                     /locus_tag="ACHOQW_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP9572121.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21602..22474
                     /gene="copD"
                     /locus_tag="ACHOQW_001007"
     CDS             21602..22474
                     /gene="copD"
                     /locus_tag="ACHOQW_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP9572122.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            22487..22828
                     /gene="yebY"
                     /locus_tag="ACHOQW_001008"
     CDS             22487..22828
                     /gene="yebY"
                     /locus_tag="ACHOQW_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP9572123.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            23224..23880
                     /gene="pphA"
                     /locus_tag="ACHOQW_001009"
     CDS             23224..23880
                     /gene="pphA"
                     /locus_tag="ACHOQW_001009"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9572124.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(23881..24072)
                     /gene="yebW"
                     /locus_tag="ACHOQW_001010"
     CDS             complement(23881..24072)
                     /gene="yebW"
                     /locus_tag="ACHOQW_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP9572125.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(24177..24413)
                     /gene="yebV"
                     /locus_tag="ACHOQW_001011"
     CDS             complement(24177..24413)
                     /gene="yebV"
                     /locus_tag="ACHOQW_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP9572126.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(24531..25970)
                     /gene="rsmF"
                     /locus_tag="ACHOQW_001012"
     CDS             complement(24531..25970)
                     /gene="rsmF"
                     /locus_tag="ACHOQW_001012"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP9572127.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(26051..28684)
                     /gene="yebT"
                     /locus_tag="ACHOQW_001013"
     CDS             complement(26051..28684)
                     /gene="yebT"
                     /locus_tag="ACHOQW_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP9572128.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(28653..29936)
                     /gene="yebS"
                     /locus_tag="ACHOQW_001014"
     CDS             complement(28653..29936)
                     /gene="yebS"
                     /locus_tag="ACHOQW_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP9572129.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30066..30563
                     /gene="msrC"
                     /locus_tag="ACHOQW_001015"
     CDS             30066..30563
                     /gene="msrC"
                     /locus_tag="ACHOQW_001015"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP9572130.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            30660..31358
                     /gene="proQ"
                     /locus_tag="ACHOQW_001016"
     CDS             30660..31358
                     /gene="proQ"
                     /locus_tag="ACHOQW_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP9572131.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            31378..33426
                     /gene="prc"
                     /locus_tag="ACHOQW_001017"
     CDS             31378..33426
                     /gene="prc"
                     /locus_tag="ACHOQW_001017"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP9572132.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            33618..34499
                     /gene="htpX"
                     /locus_tag="ACHOQW_001018"
     CDS             33618..34499
                     /gene="htpX"
                     /locus_tag="ACHOQW_001018"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP9572133.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34545..35918)
                     /gene="yebQ"
                     /locus_tag="ACHOQW_001019"
     CDS             complement(34545..35918)
                     /gene="yebQ"
                     /locus_tag="ACHOQW_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572134.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            36095..36886
                     /gene="kdgR"
                     /locus_tag="ACHOQW_001020"
     CDS             36095..36886
                     /gene="kdgR"
                     /locus_tag="ACHOQW_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP9572135.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(37030..37269)
                     /gene="yobH"
                     /locus_tag="ACHOQW_001021"
     CDS             complement(37030..37269)
                     /gene="yobH"
                     /locus_tag="ACHOQW_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP9572136.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            37429..37572
                     /gene="mgrB"
                     /locus_tag="ACHOQW_001022"
     CDS             37429..37572
                     /gene="mgrB"
                     /locus_tag="ACHOQW_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP9572137.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            37647..37934
                     /gene="yebO"
                     /locus_tag="ACHOQW_001023"
     CDS             37647..37934
                     /gene="yebO"
                     /locus_tag="ACHOQW_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP9572138.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         9728 a         9368 c         9813 g         9111 t
ORIGIN      
        1 tgccattttt cagttcatcg agacacctcg caagtttttt tcatccttcg ctggatatct
       61 atccagcatt tttttatcat acagcattat ctttgattca ttacgcagga gcgtcatgtg
      121 ataggcagac tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg
      181 ggcggcgtag gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg
      241 ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtac
      301 ggttttaaca ataaacaaga gcgcacgttg ttcaaagagt tgatcaaaac caacggcgtc
      361 ggcccgaagt tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc
      421 gttgagcgtg aagaagtggg ggcactggtg aaactgccgg gtattggcaa aaaaaccgcc
      481 gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt tgcatggcga tctctttacg
      541 ccagccgccg acctggtact cacgtctcct gccagcccgg cgaccgacga tgctgaacaa
      601 gaagcggttg ccgcgctggt ggcgctgggc tataaaccac aagaagcaag ccgcatggtg
      661 agcaaaatcg ctcgccctga cgccagcagt gaaacattaa ttcgcgaagc cctacgcgcc
      721 gcgttatgag gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc
      781 cggaagatgt agcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcagc
      841 cgcaggttcg ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc
      901 tcgatcattt gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaacattg
      961 tcgccaatga aatgggcgtt aatttacgca cgacttctgg tccggtgctg gaaaaggcgg
     1021 gcgatttggc tgcgatgctc actaaccttg aaccgcatga cgtgctgttt attgatgaga
     1081 tccaccgttt atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa gactaccaac
     1141 tggatatcat gattggtgaa ggtccggcgg cacgctccat taaaattgat ttgccgccgt
     1201 ttaccctgat tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt
     1261 ttggtattgt gcaacgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc
     1321 gcagcgcacg ctttatgggg cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc
     1381 gcgctcgcgg tacgccgcgc attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg
     1441 aagtgaagca cgatggcacc atctcggcag atatcgctgc tcaggcgctg gatatgttga
     1501 atgtcgatgc tgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata
     1561 agttctttgg tgggccggta ggtctggata accttgcggc agccattggc gaagaacgtg
     1621 aaaccattga ggatgtgctg gaaccttatt tgattcagca aggctttttg cagcgtacac
     1681 cgcgtgggcg tatggcgacg acgcgggcgt ggaatcactt tggcataacg ccgccagaaa
     1741 tgccgtaagt cggattggtc gctcgcaccg catccgacat tggcagtgtg cctgatgcga
     1801 cgctattcgc gtcttatcag gcatatacca gagatagcga accgtaggtc ggaaaaggcg
     1861 ctcgcgccgc atccgacatt ggcagtgtgc ctgatgcgac gctgttcgcg tcttaccagg
     1921 cctaccagag atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt
     1981 gttcagcgat agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg
     2041 cacatagcac caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgt
     2101 cagttactgc caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg
     2161 caaagcgacg cgcagtagct gcaggaataa tcagcaacga agtaataatc aacgcaccga
     2221 caaatttcat cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactc
     2281 gctgtaattt cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat
     2341 tgcgccattg ccagaacaaa atagccacca cgatgaccac gccaattgca atagagatga
     2401 gatcttctgg cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa
     2461 tgttagacat cagactaacg accaccaggc ccagcgacag ggcactgtgc gccataatcc
     2521 ctaataacgt gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca
     2581 gcagcagcgt aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg
     2641 cgacaccaag taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga
     2701 ctacaaacga acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg
     2761 gaaataataa ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa
     2821 atcgtgacga tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc
     2881 aaacattgaa ataaactccg gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat
     2941 gtgatgattc aggcacagca cttcatcggt ttttgccatc accagatgaa gatcgtgaga
     3001 aaccattaaa acgccacaat ccagttcacg acgcagttgg tcaataaggt catataacgc
     3061 cacctggcca tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggcg
     3121 atttaacaat gctcgcgcta acagtacacg ctgcgtttcg ccaccagaga gcttttgcat
     3181 cggtgcgtta atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt
     3241 atgtgtacca gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag
     3301 atacagcttc tgcggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc
     3361 atcgggtgtt accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg
     3421 cccaagtaaa gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg
     3481 gcgttggcca aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt
     3541 agtcttgcag tagtcatgaa atgttataat atcacacttc tcatattcat tacgatgatt
     3601 ggtcgcatta tgttacataa aaaaacgctt cttttcgcag cattattcgc cgctctctgg
     3661 ggcggtgcaa cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc
     3721 atcgcttctg ccattgctga tggggtaaca gaaacggagg ttttacttcc tgacggcgct
     3781 tcagaacatg attattcact gcgtccatcg gatgtaaaac gcttacagaa cgcggactta
     3841 gtcgtttggg ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca
     3901 gaagcgaagc aggtaacgat tgcgcagctt gaagatgtga aaccgctact gatgaaaagt
     3961 attaacggcg atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac
     4021 ggcgatttca acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca
     4081 atccatggaa aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg
     4141 aaggattttg aggcacaatt agcctcaacc gaaacgcagg ttggtaacga gctcgcgccg
     4201 ctcaagggga aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc
     4261 ggactgacac cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt
     4321 ttacatgaaa taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca
     4381 cagttcaggc cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg
     4441 ttggatccct tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt
     4501 caattagcca accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg
     4561 caacagatag cccgctctgt cgccctggcg tttaataatt taccgcgacc acaccgcgtt
     4621 atgttggggt cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat
     4681 caccgcgatg ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg
     4741 ctcttacctg aagccagtga gccgattgat caagctgcac aagaagatga agccattccc
     4801 caggacgaac tggatgacaa aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc
     4861 actggcgata cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc
     4921 caactggctg cggccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg
     4981 acattaaccg cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc
     5041 cgaacctatg accgtactgc cgctaacggt tttaaaatga ccagcgaaat gcagcaagga
     5101 gagtgggtta acaatctgct gaaaggtacc gtcgggggga gctttgttgc cagcgccaga
     5161 aacgccggtt tgaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg
     5221 gatttccgca aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt
     5281 gatggtaaac gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat
     5341 tattacgcta tccgcgctga agatggcaaa ttctacgatc gtaacggtac tggtctggcg
     5401 aaaggattct tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg
     5461 cgtcgtacta atccggtgac cggtcgcgtt gcaccacaca gaggtgttga tttcgccatg
     5521 ccgcagggta cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt
     5581 ggcgcagcag gttattatgt ggctattcgt catggtcgca gctacaccac gcgttatatg
     5641 cacttgcgca agattctggt gaaaccgggg cagaaggtga aacgtggcga ccgtatcgcg
     5701 ctttccggta ataccggacg ttcaaccggg ccgcatctgc actatgaagt atggataaac
     5761 cagcaggccg taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc
     5821 gatcgtcgcg aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa
     5881 ttaacatcca ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc
     5941 aagttgcgcc gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca
     6001 tggaaacgaa aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc
     6061 cgcgctactg gggagcatgg ctgggcgtag cagcgatggc gggtatcgct ttaacgccgc
     6121 caaagttccg tgatcccatt ctggcacggc tgggacgtat tgccggacga ctgggaaaaa
     6181 gctcacgccg tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg
     6241 aacgcgaagc gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg
     6301 ctgagttggc aatacgcggg ccggagaaaa ttcagccgcg cgttgactgg caagggctgg
     6361 agatcatcga agagatgcgg cgtaataacg agaaagttat ctttctagtg ccgcacggtt
     6421 gggccgtcga tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt
     6481 tccataatca gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg
     6541 gcggtcgtct gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg
     6601 ggtactgggg atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg
     6661 atttctttgc cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtgtgcc
     6721 gtgcgcgcgt tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc
     6781 aggtgcgccc accgatggat gatctgttag aggcggatga tcatacgatt gcgcggcgga
     6841 tgaatgaaga agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa
     6901 aattgctgaa aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc
     6961 ccatcaaata aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt
     7021 gcttcagaat attcgaaatc tgttgaacta tcattgaact gtaggccgga tgtggcgttt
     7081 tcgccgcatc cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc
     7141 cacggtactc atcacgtcgc cctgggtgac aatcaccagg tcaccagaca tcaagtaacc
     7201 tttatcgcgc agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc
     7261 aaagtgcacc ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg
     7321 cgacatggcg aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc
     7381 cgattcggtc atggtaatga tcgccgtaac gcctttcagg tggttagctg cgtacattgc
     7441 tgacatggca atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac
     7501 gttgatgctc gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc
     7561 tgacggatac tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag
     7621 aacggcgttt gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga
     7681 ctccatcatc tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa
     7741 cgctttctga atgccgacca gttccgggtc gccaatttcc acaccgaggt cgccacgtgc
     7801 aaccattacc acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc
     7861 ttccgcacgt tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg
     7921 acgggcatag ttcagatctt cgccacagcg tgggaaggag acagccaggt aatctacgcc
     7981 aatcaacgcc gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa
     8041 accgccgcca agtttgttga tacctttatt gttggagagg ggaccaccga cggtgacttc
     8101 ggtgaacact ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag
     8161 cagcaggatg tcaccaggca cgacgtcagc aggcaggcct ttgtagttga taccgacttt
     8221 ttctttgtcg ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt
     8281 gaggaaaact ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc
     8341 cagaatagcc acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat
     8401 tttgtgatct tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc
     8461 gataactttt tcaagattat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt
     8521 tgttctgcga agccttctgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct
     8581 tgaacatgaa atccgaatga aataacgccg cgatggcgat aaaataatct cattcggatg
     8641 tcgtataaga ttagaacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact
     8701 gcttatcaaa acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt
     8761 ttgcaccgcg tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa
     8821 gtcgagaaac catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca
     8881 gcgttgcttc ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atagcgtcgt
     8941 tttcgcgtgc cagctgcgcc agctcgacca gattttttgt tcttccggtg tgagaaatca
     9001 gcaccaccac gtctccgtcg ctacaattca tacaactcat gcgttgcagc acaatatcat
     9061 cggagtacac caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg
     9121 cggctgaaga gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg
     9181 cgcggttgat ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg
     9241 actcaaatat tttccctgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa
     9301 catagggagt gccattcgcc agactctgtg ccagatgaag tttaaaatca ggaaaaccgc
     9361 gcgtgtccat gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagtg
     9421 ccagagcagc aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa
     9481 ctttgcgctc tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca
     9541 tgatgtaaag agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt
     9601 tttgtcagat attacgcctg tgtgctgtgt taatgacaaa agcagataaa aaagttgtta
     9661 ttttttttca taacatgatc agtgtcagat ttttacccaa tggaaaacga tgattttttt
     9721 atcagttttg ccgcactttg cgcgctttta ccgtaatcgc acgggtggat aagcgtttac
     9781 agttttcgca agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct
     9841 ggcttaagta ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc
     9901 aggtctgtga cttggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc
     9961 cttccctgta tcaactggaa aaagccggtc agctcaaccc ggacacccgg attatcggcg
    10021 tagggcgtgc tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa
    10081 ctttcatgaa agaaaccatt gatgaaggtt tatgggacac cctgagcgca cgtctggatt
    10141 tttgtaatct cgatgtcaat gacactgctg cattcagccg tctcggcgcg atgctggatc
    10201 aaaaaaatcg tatcaccatt aactactttg ccatgccgcc cagcactttt ggcgcaattt
    10261 gcaaagggct tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac
    10321 cgctggggac gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg
    10381 aggagtgcca ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt
    10441 tggcgctgcg ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc
    10501 atgttgagat taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata
    10561 aagccggtca gatgcgcgac atgatccaga accacctgct gcaaattctt tgcatgattg
    10621 cgatgtctcc gccgtctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac
    10681 tgaagtctct gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat
    10741 atactgcggg cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga
    10801 acaagagcag caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct
    10861 gggccggtgt gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag
    10921 tcgtggtcta tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc
    10981 cgcagaataa actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga
    11041 ataaagttcc tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct
    11101 attcagaaac ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa
    11161 ccatgcgtgg tattcaggca ctgtttgtac gtcgcgatga agtggaagaa gcctggaaat
    11221 gggtagactc cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg
    11281 ccggaacctg gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga
    11341 atgagtttga gtaatatctg cgcttatcct ttatggttat tttaccggta acatgatctt
    11401 gcacagattg tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct
    11461 ttttttatta cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac
    11521 tcataaatcc tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag
    11581 gagcctttat gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg
    11641 agactcgctc tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt
    11701 cgcagttggc atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaagacaaag
    11761 cctctttgaa aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc
    11821 tctccgctca ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag
    11881 cgaatgcggt tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg
    11941 ggcaggatgg aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggctg
    12001 tcgggctgtc ccataacatg tttgatggtg ctctgttcct cggggtgtgc gacaagattg
    12061 tcccgggtct gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt
    12121 ctggaccgat ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg
    12181 ccgaaggtaa agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc
    12241 cgggaacatg tactttctac ggtactgcca acaccaacca gatggtggtg gagtttatgg
    12301 ggatgcagtt gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga
    12361 ccgctgccgc tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga
    12421 tcggtaagat gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg
    12481 gtggttccac taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga
    12541 ttaactggga tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc
    12601 cgaacggtcc ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc
    12661 gtgaactgct caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc
    12721 tgtctcgtta tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg
    12781 cggaaaaatc actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg
    12841 gtgggacaaa agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc
    12901 cggttgagaa ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta
    12961 tgccggcctt tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg
    13021 ggccaaaagc gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat
    13081 tggaccggtg tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta
    13141 aagtgccgtc agctatccac gtaacaccag aagcttacga tggcgggctg ctggcaaaag
    13201 tgcgcgacgg ggacatcatt cgtgtgaatg gacagacagg cgaactgacg ctgctggtag
    13261 acgaagcgga attggctgct cgcgaaccac acattcctga cctgagcgcg tcacgcgtgg
    13321 gaacaggacg tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg
    13381 caacctgtat cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga
    13441 aaaactggaa aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg
    13501 tggtaaaaaa actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc
    13561 gcgttctgga agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca
    13621 aagaagtgcc tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag
    13681 aagtcactga agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga
    13741 aagctgctac cgaagggact attccgctga ttccggggat cagcactgtt tccgaactga
    13801 tgctgggtat ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg
    13861 gcgtgaaagc cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg
    13921 gtggtatttc tccggctaac taccgtgact acctggcgct gaaaagcgtg ctgtgcatcg
    13981 gtggttcctg gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta
    14041 agctggcgcg tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatcgagg
    14101 atcgggcatt tttgtagcgt ttatttgccg ggtttaaccc tgtactttta cctgtccggc
    14161 ggcgtgcttc gcgcgttcaa tggcgtcaac aacactctct gcagtagcca gtgccacccc
    14221 cagacgacgg ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc
    14281 tacggcattc tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac
    14341 ggcagaagct gcaggaccat actgacggat cccgccaacc ggaagtccga ggaaggcacg
    14401 tacatgcagg gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg
    14461 tggacgaggg gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc
    14521 aaacagacca taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc
    14581 aagggcaagc gggctcattt gctgtggttg ccaggattca cagtagtcgc catcttcctg
    14641 gcgatgacct actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt
    14701 aatttcgaag tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc
    14761 gccttgctga gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg
    14821 ccccttgccg gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac
    14881 cgcctcgcgg aaaaggcttt cgctatcggc aaaacgataa gtggaagtgg gcagctgcag
    14941 ctcttctgcc gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc
    15001 gcaggggaca acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc
    15061 ttcgatctcc ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc
    15121 accatcaagc atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc
    15181 atagcgatcg acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt
    15241 acccagttca ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc
    15301 taataacgtc ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt
    15361 tgcgtgtgtc tttattgctg gtgttgatct ctcaaccgaa aagaaatata ctgtatagaa
    15421 tcacagttat tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga
    15481 gggcgaagag aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct
    15541 tgatacggcg gatctgcttg acacctggct gacaaattct ccagtgcaaa tggaagacga
    15601 gcaacgtgaa gccctttcgc tatggctggc agaacaaaaa gatgtgctga gcaccattct
    15661 gaaaaccggc aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc
    15721 ctcacacgcg gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa
    15781 tcattgtgct atggagaaaa acatgaaaaa aagaggggcg tttttagggc tgttgttggt
    15841 ttctgcctgc gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc
    15901 aaagtgtgaa ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca
    15961 aaatcgcgtg gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc
    16021 atgggtcagt ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt
    16081 acgtggtaaa agtgccgata ttcattacca ggtcagcgtg gactgcaaag cgggaatggc
    16141 ggaatatcag cggcgttaat gggttctgat aattcgattg accgttggcg gatgcggcgc
    16201 gagtgccttg tccgccccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt
    16261 cgcatcgggc aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa
    16321 atacccttat cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac
    16381 ggcctaccgc cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc
    16441 atcctctatg ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta
    16501 aatcaactgc aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag
    16561 ggattgggca aacttttagt gccgggcgca ttaggcggac tggctggact gctggtcgca
    16621 aataaatcag cacgtaaact tcttacgaaa tatggcacca acgcgttgct gattggcggc
    16681 ggagcggtag cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat
    16741 caggacgaac cgcagtttgg ctcgcaaagt acgccgctgg atgagcgtac ggaacgtttg
    16801 atccttgcgc tagtctttgc cgctaaaagt gatggtcata ttgatgccaa agaacgtgcg
    16861 gcaatcgacc agcaattgcg tgaagccggc gtggaagagc aggggcgggt actcattgag
    16921 caggcaatcg aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa
    16981 gaggcgctgg aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa
    17041 cgcagttacc tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc
    17101 attgaacgcg atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat
    17161 agcctgaagt tgactatttc gggctatcca gaacagcaca gccatttgaa caggctgatt
    17221 ttctctgtct gcataacatt cgatattcgc gccattacct gtcatcatct aagcaatgac
    17281 tgccctgttt cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttcaaccag
    17341 aaagaacaat aacatgctac caaaagccgc ccgcattccc cacgccatga cgcttcatgg
    17401 cgatacgcgc atcgataatt actactggct gcgggacgat acgcgttctc agccggaagt
    17461 cctggactac ctgcaacaag aaaatagtta cggtcatcgg gtgatggcct cacaacaagc
    17521 cttgcaggat cgcatcttaa aggaaatcat cgaccgcatt ccgcaacgag aagtttctgc
    17581 gccctacatc aaaaatggct accgctaccg gcatatttat gaaccaggct gtgaatatgc
    17641 tatctaccag cgtcaatcgg cgttcagtga agagtgggac gagtgggaaa tattgctcga
    17701 tgccaacaag cgcgcggctc atagtgagtt ttattcgatg ggcggaatgg cgattacgcc
    17761 cgataacacc attatggcgc tggcagaaga ttttctttcc cgacgccagt acggcattcg
    17821 ttttcgtaat ctggaaacag gtaactggta cccggaactg ctggataacg ttgaacccag
    17881 ctttgtctgg gcaaatgact cctggacttt ctactatgtt cgcaagcatc cggtgacgct
    17941 gctgccttat caggtctggc gtcacgccat cggtacgcca gcatcgcaag ataaactgat
    18001 ttacgaagaa aaagacgata cctattacgt cagcctgcat aaaacgacct caaagcacta
    18061 tgtagtcatt catttggcca gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat
    18121 ggcagatgcc gagccgtttg tttttctgcc gcgccgcaaa gatcacgaat acagccttga
    18181 tcactaccag catcgttttt atctgcgttc caaccgccac ggcaaaaact ttggcttata
    18241 ccgtacccgt atgcgtgatg agcaacagtg ggaagagtta attccgccac gcgaaaacat
    18301 catgctggaa gggtttacgc tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg
    18361 gttaaccagt ttgcgccaaa ttaaccgcaa gacccgggaa gtcattggta ttgcctttga
    18421 tgatccagcc tatgtgacct ggattgccta caatccagaa cctgaaaccg cgcgattgcg
    18481 ttatggttat tcttccatga ccacaccaga cactttgttt gaactggata tggataccgg
    18541 tgagcgtcgt gtattaaaac aaacagaagt tcctggtttt gatgcggcga attaccgcag
    18601 tgaacacctg tggatagtcg cccgtgatgg tgtcgaagtt ccggtttcgt tggtctacca
    18661 tcgcaatcat tttcgcaaag gacacaaccc gctgctggtg tatggctacg gttcttacgg
    18721 cgcaagtatt gatgccgatt tcagttttag ccgcttgagt ttgttagatc gtggctttgt
    18781 ctacgccatt gtccatgttc gcggcggcgg tgagctgggg caacaatggt acgaagacgg
    18841 taaatttctg aagaagaaaa atacgtttaa tgattatctt gatgcctgcg atgcattgtt
    18901 aaaactgggc tatggctctc cttcgctttg ttatgcgatg ggcgggagtg cggggggcat
    18961 gttgatgggc gttgcaatta atcaacgccc ggaattattc cacggcgtta tcgcccaggt
    19021 accgtttgtt gatgttgtaa caacgatgct tgatgaatca attcctctta ccactggtga
    19081 gtttgaagag tgggggaatc cgcaggatct gcaatattac gagtatatga aaagctacag
    19141 cccgtatgac aacgtcaccg cacaggctta tccacattta ctggtaacga ccggtttaca
    19201 cgattctcag gtgcaatatt gggaaccggc aaaatgggtc gctaagttgc gcgagctgaa
    19261 aaccgataac catcttttat tgctctgtac cgacatggac tcaggccatg gcggtaaatc
    19321 tggtcgcttt aaatcgtacg aaggcgtagc gatggaatat gcttttctgg tcgcgctggc
    19381 gcagggaaca ttacccgcta ggcctgcgga ttaggtattt tccagataat gtttcagtgt
    19441 taaacgcagc tccgggctca tgctgtccag gttattaaac aaccagcgca gatagcccgg
    19501 atcgcgttcg gcaacgtcgg aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt
    19561 catcaacgac ggacgtccgg ttatatcggc catctgttct gccgtccagc cggaggtgtt
    19621 cataatatcg ataagcaacg cggcggtgat ataacagtca tacaacgcgc ggtgatgatg
    19681 caggcccggc ggcgtctgta cattgagctt ccgtgtttta tataacgcca tattgctgta
    19741 cttgatccct ggccacaaac gacgggccag tttcatagtg caaatccact caccgggcat
    19801 ctcaggcagt acgcggcggt caaagctggc gttatgcgcg acataccatt cactaccgta
    19861 atagtgtggg atcacatctt caatccacgg tttatcggcg accatggctt cggtgatgcg
    19921 atgaatcgcc atcgcttgtg gactaatagg acgatcgggg tgcaccaggt ggctcatggg
    19981 gttgacgatt ttcccgtcaa tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa
    20041 accgcaggtt tctgtatcga taatgcgcaa catggcatgc tccaggccga aaaagtctag
    20101 cgtaatggaa tgatatcgcc ttgccaaccc tgtgatgaac gctgaccgac taacaataat
    20161 gaaccacgat cggcgcgaac gatgagacga ccatgttcat cccatagtgc actgttacca
    20221 cgggcatttg ccatcagcac agcaatcgag tactgatgtg aaaaaaattg taagcgacgg
    20281 gccgatgcca gcaggtcggg ttctcccaga cattgccctg tcgtaaacag tgaacaggtc
    20341 gggtccatat ccataccttg cggttgttca tcgaccaccg tgatggttct ggaacgacgg
    20401 cccagacatg caccatgact ctgatggtag atcccaggcg tttttcgcca cggtgcgaac
    20461 accgcaattc cacgaataaa gcgatcgtta tattcaacgg gaaggccggc aatgatggtc
    20521 attcgccagg tcgttgcggc ataacaaagc gggtctaaca gtgagagatc gggtggggca
    20581 ggaagggcac gtcgcgaata atcacacccc agtaaagaaa gcgatggaaa aactaacagc
    20641 tgacattgtt gcctggcggc ggccctcaca aactccagat gatgagctac ctgttcggtg
    20701 agtgaggttt tacgaggctc atattgcgct gctgcaactt tccagaacga cataatgact
    20761 tccttttcaa aatgactgca aattatgggt gcggtttaga aaatactcat aatttagcag
    20821 gcgttatgta agaaagtgta actctgtata agttttattt aagtttgggc tcgtaaggta
    20881 aacgtgacag attgaccgaa gccaaacggt gggcaataag ccgctcgcga aaccagctgc
    20941 gcaaatgttc aggctgttca cgttcaaccg cttcagcgat cactggcata ttgtagcgtt
    21001 ctttaaatgc caccccggcc gccgccaaat cgacattcac tttatccatt tctgtttgat
    21061 ccagtttagc cagattcttc agcattctta atctcctcat cattcgcggg caaaagttac
    21121 taagagtgag cctggatggc aagccggaat tgatgatcct cgactcagac attagacaag
    21181 gttatgctct gtaaaaaagt tattacaaaa aggaatagat aatatggctt caactgcacg
    21241 ctcccttcgc tacgcgctgg caatcctgac cacttcgttg gtgacacctt ccgtctgggc
    21301 acatgcgcat ttaacgcatc agtatcctgc ggcaaacgcg caagtgacag ctgcaccgca
    21361 ggcaatcact ttaaacttct cggaaggtgt tgaaacagga ttcagtggtg caaaaatcac
    21421 ggggccaaaa aacgaaaata ttaaaacatt accggcgaag cgaaatgagc aggaccaaaa
    21481 gcgactgatt gttccactgg ccgattcgct gaaacccgga acctacaccg ttgactggca
    21541 tgttgtttcg gtggacgggc ataaaacgaa aggacactac acctttagcg tgaaataaat
    21601 catgctggcg tttacctgga tcgcgctgcg atttatccat ttcacctcgc tgatgctggt
    21661 ctttggcttc gcaatgtacg gtgcctggct ggcaccctta acgattcgtc gcttgctgac
    21721 gaagcgtttt ctgcgtttac aacagcacgc tgccgtctgg agtttgatca gtgccactgc
    21781 aatgctcgcc gttcagggcg ggttgatggg aacgggatgg aaagatgtat tttcgccaaa
    21841 catttggcag gctgttttgc aaacgcagtt tggtggcgtc tggttatggc aaattgttct
    21901 cgccctcgta acgttgatcg tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt
    21961 tatgctcact accgcgcaat ttattctgct ggcgggagtg gggcatgcga cgctgaatga
    22021 aggggtaacg gcgaaaatcc atcagactaa tcacgcgatt cacctgattt gtgcggcggc
    22081 gtggtttggg ggattactgc cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg
    22141 acaccaggct attcaggcgc tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg
    22201 cgtactggca agcggcgtgc ttaatgcatt gttgattacc ggatttcccc ccacgcttac
    22261 gacttactgg ggccaactct tattgctcaa agccatcctg gtgatgatta tggtggtcat
    22321 tgcgctggct aatcgttatg ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc
    22381 accctggttt gtgtggatga ctaaactcga atgggcgata ggtgcggtag tgctgctgat
    22441 catcagcctg cttgcgactc tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag
    22501 tattctggcg tttctgttac tcaccagttc tgcagcggcg ctggctgcac cgcaggtgat
    22561 taccgtcagc cgttttgaag tgggtaaaga caaatgggcg tttaatcgcg aagaggtgat
    22621 gctgacttgc cgaccgggta atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta
    22681 tcctttaaac gatgtcgcac agaaggaagt tgccagtggg aagactaaag cccaacccat
    22741 ttcggtgatt cagattgatg atcccaacaa tcccggcgaa aaaatgagtc tggcaccgtt
    22801 tatagaacga gctgaaaaac tctgttaatt acctaaaata gccttttgat ttccaataaa
    22861 aaaaccgcct cagttctttc accagaacgg gcggttttta acatttaagc tgatgaccac
    22921 cgcgcttttt attgaccatt ttgcacgcaa actggaaaac ctggcgtcgt catctattct
    22981 taaagggcaa ggcaactaag cctgcattaa tgccaacttt tagcgcacgg ctctctccca
    23041 agagccattt ccctggaccg aatacaggaa tcgtgttcgg tctcttttta tctgttaaaa
    23101 gccagaagca tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga
    23161 gtccagcgtt ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata
    23221 ttgatgaaac agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc
    23281 tggctttctg gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt
    23341 cgttttgatc cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg
    23401 caaagtttac gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat
    23461 catgaacaga tggcgatgga tgcgctggca tcccagcaga tgtctttgtg gttgatgaat
    23521 ggcggcgact ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg
    23581 gaaaaatgtc agcatttgcc ctttattctt gaagtacaca gccgcaccgg caaacatgtt
    23641 attgctcatg ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat
    23701 caggtcttgt ggagccgctc gcgattaagt gaacgccaaa aagggcaggg aattacaggt
    23761 gctgatcatt tctggtttgg tcatacaccg ttgcgccatc gcgtggatat tggcaacctg
    23821 cattatattg ataccggcgc tgtctttggg ggcgaactga ctttcgtgca attgcaataa
    23881 ttaaaaatca ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg
    23941 aaaacaaccg ccatgacgga tccgttgatc gctcatagaa taaagacact gctgttccgt
    24001 gttgtagaca tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac
    24061 cagcgcgaac atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt
    24121 acaacggggg aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag
    24181 gcaagacgca taatccaggc atcagattgt tgatcgtaac gggtacggta aaggacagag
    24241 tgttcgccat ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata
    24301 cataaatcgg ggtctgattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg
    24361 tgtaattcgc cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg
    24421 ctctccattt agctgccggt tgtggtacga ccagtccgat cgcaccattt ttcagcctag
    24481 ttgaggccgc cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta
    24541 ctggtaaaaa gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa
    24601 ccaatccgtt tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc
    24661 actggcgcgg cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcttgcggt
    24721 gtcagttcaa aagcgttctc gttgtcgggg gaggcaaggg caataaccgc ttcatgctgc
    24781 cagcgataac ctttgttgtg cgtttcggca agtttaatcc ccaggcgaga aaatcggact
    24841 ttaccgatca gggcttcaat gcctaccgga aacaaccaca cttctttgtc gcgctgccag
    24901 agtcgcaggt ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtgcg
    24961 gcttcgcgat ctttcaccgg gctgaacggg aaattgccca ctttgtattt tggggtgggt
    25021 aaggcgggaa tggcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa
    25081 atttgtggga aaacatgcaa aaagccttct tcggtcagcg ctttgtttgc accagggaag
    25141 agatcgccaa gtggtaaaaa ctctactgcg tcggggtaag tctctttaag ccacaggcaa
    25201 acggattcgt tttcttcccg gtttaaggta caggtcgagt aaaccagtgt accgccagag
    25261 cgtaatgcat gaaaggcact gtcgatcagt tcccgttggg tagctgcgat ttcctgattg
    25321 ctttctggtg accaattttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag
    25381 cagggagcgt ccagcaaaat ggcatcgaac atttctggca atgccgcacc aaacacgcgg
    25441 ccatcaaaat gtgtgagcgc aacattactg ataccacagc ggctgatatt ggcatgtaac
    25501 acttttaccc gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc
    25561 gcggcaattt gcgtcgtttt ggagcctggc gcagcagcga catccatcac ccgctgtggt
    25621 gcattaccgt cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa
    25681 aacaggccac ttaaatgctc ggcggtacta cccaatggca atgcatcttc atcgtcgcgt
    25741 tcaatccaga aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg
    25801 gttaatcgca ggaaatcagc aacggaggtt ttcagcgtat taacgcgaat gctgcggcgc
    25861 aacgggcgct gacaggcggc aagaaaatca tcaaaagaga gcgtcgaagg cattgcttcg
    25921 cgcatttgcg tcagaaaggc gtccgggaaa taaacggtgt gttgggccac gagcatatac
    25981 cactggcaaa aaaaacaggc gcgcagttta gcataaacgc tccggcgcag gcacgccgga
    26041 gcagtgggca ttatttggga agcgcagttc cccattcacg ccactctttc ggttcacttt
    26101 cctgcaacag gaagtgcttg ccttcctggg ctttcggtgc cagtggcgta cccggaggcg
    26161 tggcgaaggc gatgccgcca cggataaact ggttaaaggt gccggttttc actacgccgc
    26221 ccgtcagacc aaagtccaga ctgtaacccg atgccaacca gaagacggaa ttgttacgca
    26281 ccaggtgttg atagcgttta ctgatgcgca tcgcaatcat cacgcgatct gacaatgtcc
    26341 ccagcgtcat tccggtaacc gtaccgactt ccagaccacg gaacagcaca ggcgtaccga
    26401 tgcctaacga acaggcttcc ggcgcttcaa caataatgct taaaccatcc aggtaacgcg
    26461 aatcagtaat ggtggcctct tgtaattcaa agtcgcgacg aggattgcca cggcctggtt
    26521 cgacgttgat atacggctgg aggatagtat caagatgctc aacgccagct gccgaaattt
    26581 gcggtgtgac cactgagaag cgcgtaccac cgcgagcaaa ggtctggaca tattccggat
    26641 agagcaccgc ctttgcctgt acttcattgc gcgcggtaat cagatccagc gtctggattt
    26701 gcccgatatc aataccgaga tagcgaattg gcatcccgac cgccagtttt ccggcatcga
    26761 aagcatgaag cgtaatctgc ccgccaaccg cacgggccgc tgtttcggaa gcatacagaa
    26821 tacgcttatc gcctttacgc tgactggcgc tggcaccgct gaggttatcg aagctaatgg
    26881 ctccctttaa tgctctggag agtggggatg cctgtacggt caggccacta ccattcagct
    26941 gaactttcgc cccgccttct gcccagaaca cgctattgtt ggtcagaagg ttgcgatact
    27001 ccggcttaat atgcagatcg atatcaaacg cgttagctcg cggacgcacg gtaataactt
    27061 caccaacttc aaatttacgg tacagcacta ccgatcctgc ctgcacatcc ggcagcgtct
    27121 ctgcactcaa actcacggtt gtggtgggta aatcgctaag gctgttctcc agcgcttttt
    27181 ccagattggc atacagtgga tagctggctt tcatctcacc tttatcgccc ggcagaatac
    27241 gtatcccgcc attgatccat tctgaggcgc tggcaccgag aaactcaacg ccatccagcc
    27301 ccaccttcac atcgacacgg ctgttgacga caaatttgct atcgcctttt accagttcgc
    27361 gatgctgagg ctcaatggcg acggtaaagg taacgccttt gctggtgagt ttacgatcaa
    27421 taacctggcc tacctgcacg ccgtgaagaa tgagcggctg accagcatca ataccgtaac
    27481 tttccggtgc tgtcagggtc agtgtcagaa catcaggttc atgcagcagt gctttttcgc
    27541 ctggcacaac aacgaactct ttgcgtggct cgccatcgcc aggcaccaac tcaaaggttt
    27601 ttccggtcag cagggcgctg agattggcat cgctaaggga taatttcggg ttgcgtaatt
    27661 cgatgcgggt attttcccgt aacagcgtaa cgacgctggg atcaacggtc atttctccgg
    27721 tgactttacc accaggattt aaatccagtt tagtcagctg tccgacttcc agcccctgat
    27781 acattaacgg cgtcgagtcg gcggttaatc cggccccact cggcagttcc agttttatta
    27841 ttacgccacg ctggctgtgg gccagatctt catacagacc aaaggtatct tccgcctcgg
    27901 caggtttcga ctcttctggt gaatcgaagg caatcgcacc gttaaccagt gccgccagac
    27961 tttccagttt caccttcgcg ccactgatac tgacgttggc atcaacgcct gaaacgttcc
    28021 agaaacggct accttttttc accaggtcgg taaaacgccg ctcgatcagg acatcaatca
    28081 ccacgccttg tttattggga ttgatggcat agtcgtagac ttttcccacc gggatcttgc
    28141 ggaaatagac caatgaaccg ctgttcagcg aaccgagatc gggagcttgc aggtggatca
    28201 tcagatcgcc gttatccagc cgatacttcg gttgggtatc gagcgcgaca aagtgatcct
    28261 gctctttacc tttacctggc atcatgccga tatagttacc accgacgagg gcgtccagcc
    28321 cggagacacc tgccaacgat gcttttggcg tcaccagcca gaactgagtc tcttcgcgca
    28381 gcgcatcttt catatcggac ttgatgctga ccttgacttc aatcttacga agatcgtcgc
    28441 tgaggctgat atcctgcact gttccgactt caacgccctg ataacgaaca ggcgtacggc
    28501 ccgggacaat accatccgcc gacataaagt cgatggtgac ggtattaccc cggtcctgat
    28561 aactgtccca aatcagccaa ccggcaatca ttagcgcgat gaacggcagc agccagaaag
    28621 gtgagatacg gcgtttattt ttaatctgcg cttcagtcgt cgaagcgggc gtttcctgac
    28681 tcatgtgcat cccaaagtaa gcggctgtcc agccattcca cagcaagaat agtcaaaatt
    28741 accgctgcgc cgaaataaaa cgcagccggt cccatagtaa aagcgaggat ctgatcgcga
    28801 ttaatcagcg acatggttaa agatatgaca aacaggtcga gcatcgacca gcgaccaatc
    28861 caggtcacca tccgcagtaa cagaatgcgt gtgcgtaaac cttgctggca tttaaaatga
    28921 atgctcaaca gtaaagtgaa catgacgatc actttagtaa acggtaccag aatactggcg
    28981 ataaacacga ttcctgcaac cgcaatgttg ctacttgcca gcgacataat tccggaaaga
    29041 attgtatctt cctgccgtcc tccgttcaga taaatgatag aaataggcaa caggttggca
    29101 ggtaacaata aaacgattga cgctaacagc gccgcccagc atttttgcag actatgacgg
    29161 cgacgcaggc gtagcggaat atgacaacgc gggcagcgac cacgctgatc aggatagccg
    29221 gtaaaatggc acccaagaca gacacgaagt ttctcgtccc tacgcgtagc ggggcgctgc
    29281 ggataaaatc gctcccacag ctcctcgaca ttaagatgtg acaacgtcac cgtcgttaaa
    29341 atcaccaacg ccacaaaaga gaacaagccg acacccgcct ggatatgggc ataatcctgt
    29401 acctttatag aagcaacgcc aatgccgacc aggtagatat cgagcattac ccactcttta
    29461 agtcgctcaa gcatcagcag taccgggcgt agattcattc ccagtcggtt accaaaccat
    29521 aaataagcta tggaggtcac cagaatgagc ggggcaccaa taacgcagaa aaagaccatc
    29581 gaccccgtta tcgcatcgcc ctgtttggtc atttgccaga tgccttgcat aacgttggcg
    29641 tcaatacgaa tgcctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcatc
    29701 agcaacagca tggtgaaggc cattgccgcc aggcgcgtta gcgaccagtc gcgcccgtca
    29761 cgaatttttg cctgacagcg cggacaatag gcactttgat gagaatttat ctcgggcagg
    29821 ctaaacagca tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg
    29881 attgccctca ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg
    29941 tgccaaatgt aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat
    30001 taacgcattt aatgcttatt ttaataggct ggcagttcca ggtaagacaa ccaagtgatt
    30061 atataatgaa caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg
    30121 cgggagaaac cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tatgagcgtc
    30181 tcactgacgt aaactgggca ggtttttatt tgcttgagga cgatacactg gtgctcggac
    30241 catttcaggg caaaattgcc tgtgtccgga tacccgtcgg gcgtggcgtg tgcggcactg
    30301 cggttgcccg caatcaagtg cagcgtatcg aggatgttca tgcgtttgac gggcatattg
    30361 cctgtgatgc ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta
    30421 ttggtgttct cgacatcgat agtaccgtct tcggtcgctt tacagacgag gacgagcaag
    30481 gcttacgtca gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat
    30541 tctttgcgag cgtcgcagga taatcaacgg ataacgtagc atttactgat agcgtcatta
    30601 taatgacgcc tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca
    30661 tggaaaatca acctaagttg aatagcagta aagaagtaat cgcgtttctg gccgaacgtt
    30721 ttccccactg tttcagtgcg gaaggtgaag cgcgtccgct gaaaatcggt atttttcagg
    30781 atttggtcga tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt
    30841 tacgtctcta cacttcgagc tggcgttacc tttacggtgt taaacccggc gcaacgcgtg
    30901 tcgatcttga cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca
    30961 agcagcttga agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa
    31021 aacgcgaagc tgccgcagct gctggtgaga aagaagacgc accgcgtcgc gaacgcaagc
    31081 cacgtccgac cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc
    31141 cggtagagaa agcgccaaaa acagcaaaag cacctcgcga agaacagcac accccagttt
    31201 ctgacatttc agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga
    31261 tggatgccac cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta
    31321 tgtctttgat tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg
    31381 aacatgtttt ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc
    31441 gctgtagaag atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat
    31501 gcgacggtga gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac
    31561 ctcgatcagg cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc
    31621 cacaacgtgc tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc
    31681 gatgaactgc gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag
    31741 cgtcgttttg aacgttacca atacgctttg tcggtactgg aaaagccgat ggatttcacc
    31801 ggcaacgaca cttataacct tgaccgcagc aaagcgccct ggccgaaaaa cgaggctgag
    31861 ttgaacgcgc tgtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctggcagga
    31921 aaaacggata aagaaattcg tgaaaccctg actcgccgct acaaatttgc cattcgtcgt
    31981 ctggcgcaaa ccaacagcga agatgttttc tcgctggcaa tgacggcgtt tgcgcgtgaa
    32041 atcgacccgc ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg
    32101 agtttgtcgc tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc
    32161 aattcgatgg tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa
    32221 attgtcggtg ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat
    32281 gatgtggttg ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct
    32341 gctggtaaag ggaccaagac ccgcactgta acattgaccc gtgaacgtat tcgtctcgaa
    32401 gaccgcgcgg ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat
    32461 attcctggct tctatgtggg tttgacagac gatgtcaaag tgcaactgca gaaactggaa
    32521 aaacagaatg tcagcagtgt gatcatcgat ctgcgtagca atggtggtgg ggcgctgacc
    32581 gaagcggtat cgctctcagg tctgtttatt cctgcgggtc ctattgttca ggtccgtgat
    32641 aacaacggta aagttcgtga agacagcgat accgacggac aggttttcta taaaggcccg
    32701 ctggtggtac tggttgaccg cttcagtgct tcggcttcag aaatctttgc cgcggcaatg
    32761 caggattacg gtcgtgcact ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag
    32821 caataccgtt cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg
    32881 ggttctgtgc agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt
    32941 aaaggcgtaa cgccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag
    33001 aaattcgaag ataacgcgct gccgtgggat agcattgatg ccgcgactta tgtgaaatca
    33061 ggcgatttaa cggcctttga accggagcta ctgaaggaac ataatgcgcg tatcgcgaaa
    33121 gatcctgagt tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag
    33181 cgcaatatcg tttctctgaa ttacgctgtg cgtgagaaag agaataatga agatgatgcg
    33241 acgcgtttgg cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaaa
    33301 ctggatgatc taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat
    33361 atcgcactcg atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgcacccgtc
    33421 aagtaatatc aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg
    33481 taaatcagat gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga
    33541 cttgaaaata gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact
    33601 gaggtaaaaa gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa
    33661 tggtcgtttt cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc
    33721 tgatgatcat ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca
    33781 aatggatggc attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg
    33841 aacgttggct ggtcaatact gtagcaaccc aggctcgtca ggcggggatc gctatgccgc
    33901 aagtggctat ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg
    33961 cctctctggt tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg
    34021 cggtaattgc tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga
    34081 ttcagggcgt ggtgaacacc ttcgttatct ttatttcccg tatcctggcg cagcttgccg
    34141 cgggttttat gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct
    34201 actttgcggt tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca
    34261 tgtggttctc gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc
    34321 gcgagaaaat gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa
    34381 ccagcatgat ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgtttatga
    34441 ctcacccgcc gctggataaa cgtattgaag ctctgcgtac gggtgaatac ctgaagtaat
    34501 atgaagtaaa tactaaaaag cgcgtcctgc tcgacgcgct ttttttatgc cctggatctt
    34561 ggctgagtga tacgtaaccc actgatacag gcagcaatca ctgccagaat agccgcagcc
    34621 atcagcgaga cgtgtgtacc attatctcca aactgattta gcatcagcgc caccagcgcc
    34681 gcgccgctac tctgacccag tagacgagcc gttcctaaca tgccactggc tccaccgcta
    34741 cgttcgcgag gcgcggaggt aataatggtg tggttattgg gtgactggaa caagccaaat
    34801 ccagcgccac ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc
    34861 agaaccaggg aaaaaagccc cgcagccatg atgaataatc ctaaagcacc cagcaatcct
    34921 gcatgtacgc gttcaatcaa atagcctgcc aacggagcca tcaccatcgt tgctaacggc
    34981 cacggtgtca gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa
    35041 aagggcaggg aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata
    35101 gaaagtgaaa acagcgggat acgcagtaaa tccaccggta gcagcggtac tggaagagaa
    35161 agctggcggc gaataaagaa aataccaacg acaaccatta caaccagttc cgcagcaatt
    35221 aacgtcagcg attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt
    35281 aacgcgttca tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccattg
    35341 ggtggcagaa aacgcatcgc cagaagcagg gcgataatac ccaacggtac gttgattaaa
    35401 aataaccatt tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca
    35461 gaagagacgg caacaataaa cgagtttatg cccatccctc tacccagaaa acgttgtgga
    35521 tagatcaggc ggataagggc ggtattaacg ctcatcaacg ctgcaccgcc gaaaccttgt
    35581 atgacacgcg caagggtgag tatttgcagc gaatcagaaa gggcgcagaa cagtgaagac
    35641 aacagaaaaa cgaccagacc gcatttataa atacgtcgat agccaaacat atcacccaga
    35701 aacgaaaacg agagcaggga gatgacaatg gcgatttgat aggcgttcac tacccagatg
    35761 gaactggctg gcgtggcatg aaggtccgtg gcgattgttg gcagggcgac gttggcgatt
    35821 gcgccgtcaa ggacagccat cgaaatacca atcacaatgg ttaatatcgc accgtatcgc
    35881 tggggcaatg gcaggccgtc ggcctgaact tttggcatag gaattttaaa tctttggtga
    35941 ataatcaaca ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa
    36001 ctaaccggca aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc
    36061 tgatttttat aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca
    36121 gcctgattct gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga
    36181 gcgcgaaata gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta
    36241 tcgcttttta cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa
    36301 atattccctg accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt
    36361 aattcgtagc gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca
    36421 cctcggcgca ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa
    36481 tttgcgcatg tattcacgga ttgggcgtcg taatccactg tacagcaccg cgattggtaa
    36541 ggtactgctg gcatggcgcg atcgcgatga agtgaagcaa attcttgagg gcgtggagta
    36601 taaacgcagt accgagcgga ccatcaccag tacagaagcg ttattaccgg ttctggacca
    36661 ggtgcgcgag caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat
    36721 tgcggttccg gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc
    36781 gacgttgcgt ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc
    36841 gcgcaaaatt tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc
    36901 tgtcggaata tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg
    36961 gcgttagatt aaacgcagtt gatataagaa tattttactc atgagtgaga atctggttat
    37021 ttattacact taaccattat cgaccacgat attgcttttg cgtaacagcg ggcaatctgt
    37081 tatccccaaa aaaccacttt tagtgtgcaa gtattgtacc gtgctggtgc ctctggcagt
    37141 cagataggta cattgcaaac ctaattctgc ggcattctct ttgcttccaa tcaaaacgcc
    37201 atatccgctg agtaataatc ctatccatac cagtgctatc agcataactg tgcgaatgat
    37261 gaatcgcatt acaacctctt ctctttttta tgttcgctta atcgtagcgg caatatgcgc
    37321 tgaagcaagc gactcattcc gaaaaagcac gaatatcgac atagttagac gctgtttaac
    37381 taacgcatgg tagtttaatg acataaggta ggtgaaacgg agattggagt gaaaaagttt
    37441 cgatgggtcg ttttggttgt cgtggtgttg gcttgcttgc tgctttgggc gcaggtattc
    37501 aacatgatgt gcgatcagga tgtacaattt ttcagcggaa tttgtgccat taaccagttt
    37561 atcccgtggt gatatcattt tttcagcact agattttctt cctctccagc agtggtagaa
    37621 tagcgcctct actcagaggt ggtgaaatga acgaagttgt aaattcaggc gtgatgaaca
    37681 ttgcgtcttt ggttgtatcg gtggtggttc ttcttatcgg gctcatcttg tggtttttta
    37741 ttaatcgtgc cagttctcgg actaacgaac agattgaact gcttgaggcg ttgctggatc
    37801 agcaaaaacg tcaaaatgca ctgttacgtc gtttgtgcga agcaaacgaa ccagagaaag
    37861 cagataaaaa gactattgag agtcaaaaat cggttgaaga cgaagatatt attcgcctgg
    37921 tcgccgaacg ataatccttc ctcaatcttg agtaaccccg tcgttgaagg gttactcaat
    37981 tcatcctgat tatacgcact gtcatggtgc aactgcttca
//
LOCUS       DAZKFZ010000021        37802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000021 DAZKFZ010000000
VERSION     DAZKFZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            103..507
                     /gene="ybgC"
                     /locus_tag="ACHOQW_001024"
     CDS             103..507
                     /gene="ybgC"
                     /locus_tag="ACHOQW_001024"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9572139.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            504..1196
                     /gene="tolQ"
                     /locus_tag="ACHOQW_001025"
     CDS             504..1196
                     /gene="tolQ"
                     /locus_tag="ACHOQW_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP9572140.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            1200..1628
                     /gene="tolR"
                     /locus_tag="ACHOQW_001026"
     CDS             1200..1628
                     /gene="tolR"
                     /locus_tag="ACHOQW_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP9572141.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            1693..2907
                     /gene="tolA"
                     /locus_tag="ACHOQW_001027"
     CDS             1693..2907
                     /gene="tolA"
                     /locus_tag="ACHOQW_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP9572142.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAE
                     ADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESK
                     FYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVY
                     EVFKNAPLDFKP"
     gene            3040..4332
                     /gene="tolB"
                     /locus_tag="ACHOQW_001028"
     CDS             3040..4332
                     /gene="tolB"
                     /locus_tag="ACHOQW_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP9572143.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            4367..4888
                     /gene="pal"
                     /locus_tag="ACHOQW_001029"
     CDS             4367..4888
                     /gene="pal"
                     /locus_tag="ACHOQW_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP9572144.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            4898..5689
                     /gene="cpoB"
                     /locus_tag="ACHOQW_001030"
     CDS             4898..5689
                     /gene="cpoB"
                     /locus_tag="ACHOQW_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP9572145.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            5854..5929
                     /locus_tag="ACHOQW_001031"
     tRNA            5854..5929
                     /locus_tag="ACHOQW_001031"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5887..5889,aa:Lys,seq:ttt)
     gene            5965..6040
                     /locus_tag="ACHOQW_001032"
     tRNA            5965..6040
                     /locus_tag="ACHOQW_001032"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5998..6000,aa:Val,seq:tac)
     gene            6043..6118
                     /locus_tag="ACHOQW_001033"
     tRNA            6043..6118
                     /locus_tag="ACHOQW_001033"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6076..6078,aa:Lys,seq:ttt)
     gene            6170..6245
                     /locus_tag="ACHOQW_001034"
     tRNA            6170..6245
                     /locus_tag="ACHOQW_001034"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6203..6205,aa:Val,seq:tac)
     gene            6249..6324
                     /locus_tag="ACHOQW_001035"
     tRNA            6249..6324
                     /locus_tag="ACHOQW_001035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6282..6284,aa:Lys,seq:ttt)
     gene            6471..6546
                     /locus_tag="ACHOQW_001036"
     tRNA            6471..6546
                     /locus_tag="ACHOQW_001036"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6504..6506,aa:Lys,seq:ttt)
     gene            6824..7867
                     /gene="nadA"
                     /locus_tag="ACHOQW_001037"
     CDS             6824..7867
                     /gene="nadA"
                     /locus_tag="ACHOQW_001037"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP9572146.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            7905..8624
                     /gene="pnuC"
                     /locus_tag="ACHOQW_001038"
     CDS             7905..8624
                     /gene="pnuC"
                     /locus_tag="ACHOQW_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9572147.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(8621..9562)
                     /gene="zitB"
                     /locus_tag="ACHOQW_001039"
     CDS             complement(8621..9562)
                     /gene="zitB"
                     /locus_tag="ACHOQW_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP9572148.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(9676..10056)
                     /gene="ybgS"
                     /locus_tag="ACHOQW_001040"
     CDS             complement(9676..10056)
                     /gene="ybgS"
                     /locus_tag="ACHOQW_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP9572149.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            10372..11424
                     /gene="aroG"
                     /locus_tag="ACHOQW_001041"
     CDS             10372..11424
                     /gene="aroG"
                     /locus_tag="ACHOQW_001041"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP9572150.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(11590..12342)
                     /gene="gpmA"
                     /locus_tag="ACHOQW_001042"
     CDS             complement(11590..12342)
                     /gene="gpmA"
                     /locus_tag="ACHOQW_001042"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9572151.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12544..13584)
                     /gene="galM"
                     /locus_tag="ACHOQW_001043"
     CDS             complement(12544..13584)
                     /gene="galM"
                     /locus_tag="ACHOQW_001043"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP9572152.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(13578..14726)
                     /gene="galK"
                     /locus_tag="ACHOQW_001044"
     CDS             complement(13578..14726)
                     /gene="galK"
                     /locus_tag="ACHOQW_001044"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9572153.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(14730..15776)
                     /gene="galT"
                     /locus_tag="ACHOQW_001045"
     CDS             complement(14730..15776)
                     /gene="galT"
                     /locus_tag="ACHOQW_001045"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP9572154.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(15786..16802)
                     /gene="galE"
                     /locus_tag="ACHOQW_001046"
     CDS             complement(15786..16802)
                     /gene="galE"
                     /locus_tag="ACHOQW_001046"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9572155.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(17064..18536)
                     /gene="modF"
                     /locus_tag="ACHOQW_001047"
     CDS             complement(17064..18536)
                     /gene="modF"
                     /locus_tag="ACHOQW_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP9572156.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(18604..19392)
                     /gene="modE"
                     /locus_tag="ACHOQW_001048"
     CDS             complement(18604..19392)
                     /gene="modE"
                     /locus_tag="ACHOQW_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP9572157.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            19521..19670
                     /gene="acrZ"
                     /locus_tag="ACHOQW_001049"
     CDS             19521..19670
                     /gene="acrZ"
                     /locus_tag="ACHOQW_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP9572158.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            19837..20610
                     /gene="modA"
                     /locus_tag="ACHOQW_001050"
     CDS             19837..20610
                     /gene="modA"
                     /locus_tag="ACHOQW_001050"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9572159.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGASADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            20610..21299
                     /gene="modB"
                     /locus_tag="ACHOQW_001051"
     CDS             20610..21299
                     /gene="modB"
                     /locus_tag="ACHOQW_001051"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP9572160.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            21302..22360
                     /gene="modC"
                     /locus_tag="ACHOQW_001052"
     CDS             21302..22360
                     /gene="modC"
                     /locus_tag="ACHOQW_001052"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP9572161.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(22361..23179)
                     /gene="ybhA"
                     /locus_tag="ACHOQW_001053"
     CDS             complement(22361..23179)
                     /gene="ybhA"
                     /locus_tag="ACHOQW_001053"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP9572162.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            23334..24329
                     /gene="pgl"
                     /locus_tag="ACHOQW_001054"
     CDS             23334..24329
                     /gene="pgl"
                     /locus_tag="ACHOQW_001054"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP9572163.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(24370..25323)
                     /gene="ybhD"
                     /locus_tag="ACHOQW_001055"
     CDS             complement(24370..25323)
                     /gene="ybhD"
                     /locus_tag="ACHOQW_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9572164.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            25507..26559
                     /gene="ybhH"
                     /locus_tag="ACHOQW_001056"
     CDS             25507..26559
                     /gene="ybhH"
                     /locus_tag="ACHOQW_001056"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP9572165.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            26635..28068
                     /gene="ybhI"
                     /locus_tag="ACHOQW_001057"
     CDS             26635..28068
                     /gene="ybhI"
                     /locus_tag="ACHOQW_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP9572166.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            28251..30512
                     /gene="ybhJ"
                     /locus_tag="ACHOQW_001058"
     CDS             28251..30512
                     /gene="ybhJ"
                     /locus_tag="ACHOQW_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP9572167.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(30653..31936)
                     /gene="ybhC"
                     /locus_tag="ACHOQW_001059"
     CDS             complement(30653..31936)
                     /gene="ybhC"
                     /locus_tag="ACHOQW_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP9572168.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(32071..33141)
                     /locus_tag="ACHOQW_001060"
     CDS             complement(32071..33141)
                     /locus_tag="ACHOQW_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9572169.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(33119..33337)
                     /locus_tag="ACHOQW_001061"
     CDS             complement(33119..33337)
                     /locus_tag="ACHOQW_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9572170.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(33377..33544)
                     /locus_tag="ACHOQW_001062"
     CDS             complement(33377..33544)
                     /locus_tag="ACHOQW_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572171.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            33787..34389
                     /locus_tag="ACHOQW_001063"
     CDS             33787..34389
                     /locus_tag="ACHOQW_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572172.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(34600..34821)
                     /locus_tag="ACHOQW_001064"
     CDS             complement(34600..34821)
                     /locus_tag="ACHOQW_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9572173.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(34920..35135)
                     /locus_tag="ACHOQW_001065"
     CDS             complement(34920..35135)
                     /locus_tag="ACHOQW_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9572174.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(35212..35403)
                     /locus_tag="ACHOQW_001066"
     CDS             complement(35212..35403)
                     /locus_tag="ACHOQW_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9572175.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(35376..35558)
                     /locus_tag="ACHOQW_001067"
     CDS             complement(35376..35558)
                     /locus_tag="ACHOQW_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9572176.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(35555..36235)
                     /locus_tag="ACHOQW_001068"
     CDS             complement(35555..36235)
                     /locus_tag="ACHOQW_001068"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9572177.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(36232..36903)
                     /gene="bet"
                     /locus_tag="ACHOQW_001069"
                     /pseudo
     CDS             complement(36232..36903)
                     /gene="bet"
                     /locus_tag="ACHOQW_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            36933..37115
                     /locus_tag="ACHOQW_001070"
     CDS             36933..37115
                     /locus_tag="ACHOQW_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9572178.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            37112..37282
                     /locus_tag="ACHOQW_001071"
     CDS             37112..37282
                     /locus_tag="ACHOQW_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9572179.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            37275..>37802
                     /locus_tag="ACHOQW_001072"
     CDS             37275..>37802
                     /locus_tag="ACHOQW_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9572180.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNR"
BASE COUNT         9240 a         9208 c         9756 g         9598 t
ORIGIN      
        1 ttttacgctc ccttaacttg ccctcattcc caaacctcaa tcgcgcgcgt atagtagcag
       61 cgtttaaaag ctctaacttt tgttgcatta ccgggatgta aagtgaatac aacgctgttt
      121 cgatggccgg ttcgcgtcta ctatgaagat accgatgccg gtggtgtggt gtaccacgcc
      181 agttacgtcg ctttttatga aagagcacgc acagagatgc tgcgtcatca tcacttcagt
      241 caacaggcgc tgatggctga acgcgttgcc tttgtggtac gtaaaatgac ggtggaatat
      301 tacgcacctg cgcggctcga cgatatgctc gaaatacaga ctgaaataac atcaatgcgt
      361 ggcacctctt tggttttcac gcaacgtatt gtcaacgccg agaatacttt gctgaatgaa
      421 gcagaggttc tggttgtttg cgttgaccca ctcaaaatga agcctcgtgc gcttcccaag
      481 tctattgtcg cggagtttaa gcagtgactg acatgaatat ccttgatttg ttcctgaagg
      541 ctagccttct ggttaaactt atcatgttga ttttgattgg tttttcaatc gcatcctggg
      601 ccattattat ccagcggacc cgtattctta acgcagcggc gcgcgaagcc gaagcgtttg
      661 aagataaatt ctggtctgga atcgaactct ctcgcctcta tcaagagagc caggggaaac
      721 gggataatct gactggttcg gaacaaatct tttacagcgg gttcaaagaa tttgtgcgcc
      781 tgcatcgtgc caatagccat gcgccggaag ccgtagtgga aggggcgtcg cgtgcgatgc
      841 gtatttccat gaaccgcgaa cttgaaaatc tggaaacgca cattccgttc ctcggtacgg
      901 ttggctccat cagcccgtat attggtctgt ttggtacggt ctgggggatc atgcacgcct
      961 ttatcgccct cggggcggta aaacaagcaa cactgcaaat ggttgcgccc ggtatcgcag
     1021 aagcgttgat tgcgacggca attggtctgt tcgccgctat tccggcggta atggcttata
     1081 accgtctcaa ccaacgtgta aacaaactgg aactgaatta cgacaacttt atggaagagt
     1141 ttaccgcgat tctgcaccgc caggcgttta ccgttagcga gagcaacaag gggtaagcca
     1201 tggccagagc gcgtggacga ggtcgtcgcg atctcaagtc cgaaatcaac attgtaccgt
     1261 tgctggacgt actgctggtg ctgttgctga tctttatggc gacagcgccc atcatcaccc
     1321 agagcgtgga ggtcgatttg ccagacgcta ctgaatcaca ggcggtgagc agtaacgata
     1381 atccgccagt gattgttgaa gtgtctggta ttggtcagta caccgtggtg gttgagaaag
     1441 atcgcctgga gcgtttacca ccagagcagg tggtggcgga agtgtccagc cgtttcaagg
     1501 ccaacccgaa aacggtcttt ctgatcggtg gcgcaaaaga tgtgccttac gatgaaataa
     1561 ttaaagcact gaacttgtta catagtgcgg gtgtgaaatc ggttggttta atgacgcagc
     1621 ctatctaaac atctgcgttt cccttgcttg aaagagagcg ggtaacaggc gaacagtttt
     1681 tggaaaccga gagtgtcaaa ggcaaccgaa caaaacgaca agctcaagcg ggcgataatt
     1741 atttcagcag tgctgcatgt catcttattt gcggcgctga tctggagttc gttcgatgag
     1801 aatatagaag cttcagctgg aggcggcggt ggttcgtcca tcgacgctgt catggttgat
     1861 tcaggtgcgg tagttgagca gtacaaacgc atgcaaagcc aggaatcaag cgcgaagcgt
     1921 tctgatgagc agcgcaagat gaaggaacag caggctgctg aagaactgcg tgagaaacaa
     1981 gcggctgaac aggaacgcct gaagcaactt gagaaagagc ggttagctgc tcaggaacag
     2041 aaaaagcagg ctgaagaagc cgcaaaacag gccgagttaa agcagaagca agcggaagag
     2101 gcggcagcga aagcggcggc agatgctaaa gcgaaggccg aagcggatga taaagctgcg
     2161 gaagaagcag cgaagaaagc ggctgcagac gcgaagaaaa aagcagaagc agaagccgcc
     2221 aaagccgcag ccgaagcgca gaaaaaagcc gaggcagcag ctgcggcgct gaagaagaaa
     2281 gcggaagcgg cagaagcagc tgcagctgaa gcaagaaaga aagcggcagc agagaaagct
     2341 gcagccgaca aaaaagcagc agagaaagct gcagccgaca aaaaagcagc agaaaaagcg
     2401 gctgctgaaa aggcagcagc tgataagaaa gcagcggcag aaaaagccgc cgcagacaaa
     2461 aaagcggcag ctgcaaaagc agcagctgaa aaagccgctg cagcaaaagc tgccgcggag
     2521 gcagatgata ttttcggtga gctaagctct ggtaagaatg caccgaaaac ggggggaggg
     2581 gcgaaaggga acaatgcttc gcctgccggg agtggtaata ctaaaaacaa tggcgcatca
     2641 ggggccgata tcaataacta tgccgggcag attaaatctg ctatcgaaag taagttctat
     2701 gacgcatcgt cctatgcagg caaaacctgt acgctgcgca taaaactggc acccgatggt
     2761 atgttactgg atatcaaacc tgaaggtggc gatcccgcac tttgtcaggc tgcgttggca
     2821 gcagctaaac ttgcgaagat cccgaaacca ccaagccagg cagtatatga agtgttcaaa
     2881 aacgcgccat tggacttcaa accgtaatcg cgatgttgac tgttccgacg gtcaacatca
     2941 ggcaccggtt gccacggggt tctggtagtt ttgtgtattt tagtttgtta acattctgct
     3001 aaattatcgt gggccatcgg tccagataag ggagatatga tgaagcaggc attacgagta
     3061 gcatttggtt ttctcatact gtgggcatca gttctgcatg ctgaagtccg cattgtgatc
     3121 gacagcggtg tagattccgg tcgtcctatt ggagttgttc ctttccagtg ggcgggccct
     3181 ggtgcggcac ctgaagatat tggcggcatc gttgctgccg acttgcgtaa cagcggtaaa
     3241 tttaatccgt tagatcgcgc tcgtctgcca cagcagccgg gtagtgcgca ggaagtacaa
     3301 ccagctgcat ggtccgcgct gggtattgac gctgtagttg tcggtcaggt cactccgaat
     3361 ccggatggtt cttacaatgt tgcttatcaa cttgttgaca ctggcggcgc accgggtact
     3421 gtacttgctc agaactcgta caaagtgaac aagcagtggc tgcgttatgc tggtcatacc
     3481 gccagtgatg aagtgtttga aaaactgacc ggtattaaag gtgcgttccg tacccgtatt
     3541 gcctacgttg ttcagaccaa cggcggtcag ttcccgtatg aactgcgcgt atctgactat
     3601 gacggttaca accagtttgt cgttcaccgt tcaccacagc cgctgatgtc cccggcgtgg
     3661 tcaccagacg gttctaaact ggcttatgtg accttcgaaa gcggtcgttc cgcgctggtt
     3721 atacaaacgc tggcaaacgg cgctgtacgt caggtggctt cattcccgcg tcacaacggt
     3781 gcgcctgcat tctcgccaga cggcagcaaa ctggcattcg ccttgtcgaa aaccggtagc
     3841 ctgaacctgt acgtaatgga tttggcttct ggtcagatcc gccaggtgac tgatggtcgc
     3901 agtaacaata ccgaaccgac ctggttcccg gacagccaga acctggcatt tacttctgac
     3961 caggccggtc gtccacaggt ttataaagtg aatatcaacg gcggtgcgcc acaacgtatt
     4021 acctgggaag gttcgcagaa ccaggatgcg gatgtcagca gcgacggtaa atttatggta
     4081 atggtcagct ccaatggtgg gcagcagcac attgccaaac aagatctggc aacgggaggc
     4141 gtacaagttc tgtcgtccac gttcctggat gaaacgccaa gtctggcacc taacggcact
     4201 atggtaatct acagctcttc tcaggggatg ggatccgtgc tgaatttggt ttctacagat
     4261 gggcgtttca aagcgcgtct tccggcaact gatggacagg tcaaattccc tgcctggtcg
     4321 ccgtatctgt gataataatt aattgaatag taaaggaatc attgaaatgc aactgaacaa
     4381 agtgctgaaa gggctgatga ttgctctgcc tgttatggca attgcggcat gttcttccaa
     4441 caagaacgcc agcaatgacg gcagcgaagg catgctgggt gccggcactg gtatggatgc
     4501 gaacggcggc aacggcaaca tgtcttccga agagcaggct cgtctgcaaa tgcaacagct
     4561 gcagcagaac aacatcgttt acttcgatct ggacaagtac gatatccgtt ctgacttcgc
     4621 tcaaatgctg gatgcacatg caaacttcct gcgtagcaac ccgtcttaca aagtcaccgt
     4681 agaaggtcac gcggacgaac gtggtactcc ggaatacaac atctccctgg gtgaacgtcg
     4741 tgcgaacgcc gttaagatgt acctgcaggg taaaggcgtt tctgcagacc agatctccat
     4801 cgtttcttac ggtaaagaaa aacctgcagt actgggtcat gacgaagcgg catactccaa
     4861 aaaccgtcgt gcggtactgg tttactaaga gaattgcatg agcagtaact tcagacatca
     4921 actattgagt ctgtcgttac tggttggtat agcggccccc tgggccgctt ttgctcaggc
     4981 accaatcagt agtgtcggct caggctcggt cgaagaccgc gtcattcaac ttgagcgtat
     5041 ttctaatgct cacagccagc ttttaaccca actccagcaa caactctctg ataatcaatc
     5101 cgatattgat tccctgcgtg gtcagattca ggaaaatcag tatcaactga atcaggtcgt
     5161 ggagcggcaa aagcagatcc tgttgcagat cgacagcctc agcagcggtg gtgcagcggc
     5221 gcaatcaacc agcggtgatc aaagcggtgc ggcggcatca acgacgccga cagctgatgc
     5281 tggtactgcg aatgctggcg cgccggtgaa aagcggtgat gcaaacacgg attacaatgc
     5341 agctattgcg ctggtgcagg ataaatcccg ccaggatgac gcaatggtgg catttcagaa
     5401 tttcatcaaa aattaccctg attcaactta cctgccaaac gccaattatt ggctgggtca
     5461 gttaaactac aacaagggta aaaaagatga tgcggcgtac tattttgctt cggtagtgaa
     5521 aaactatccg aagtcaccaa aggctgcaga tgcgatgttt aaagtcggcg tcatcatgca
     5581 ggacaaaggt gacaccgcaa aagcgaaagc cgtataccag caggttatca gtaaataccc
     5641 tggtactgat ggcgctaaac aggcacaaaa acgtctgaac gcgatgtaat gcataacaca
     5701 cgaccagaag tcgcattatt tctggtcgtg tcgtgcgaat cataagcagt tgagtgatct
     5761 acatcgaaat ttttgttgcg ctcaagtctg aaatcagtaa tatatgccgc cgttgccacg
     5821 ggatatcaaa caaaccgaaa gcaacgaaaa agtgggtcgt tagctcagtt ggtagagcag
     5881 ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccac taacctagtt
     5941 agttgtagta tccagcgtaa tatcgggtga ttagctcagc tgggagagca cctcccttac
     6001 aaggaggggg tcggcggttc gatcccgtca tcacccacca ccgggtcgtt agctcagttg
     6061 gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccact
     6121 aattacggtg ggttcggtgg aagtagtttg tagtatccag cgcagtatcg ggtgattagc
     6181 tcagctggga gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc
     6241 caccactcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag
     6301 gttcgaatcc tgcacgaccc accaatttta acatcaaact cagatgttaa gcgtgaagga
     6361 taacgttgcg tcagcaacgg cccgtagggc aagcgaagcg agtcatcctg cacgacccac
     6421 caattttaaa gattggttcc gagtagaaat ctttcaggta acacccgtat gggtcgttag
     6481 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
     6541 ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct attcaggcat ctctcaattt
     6601 caattcttga ccctgatgac atactccgcg cttgctgcgt aaacaacttt acgtcggagg
     6661 aataagacgc aaagtgtggc attcggcatc tcatacgtaa ttcgagtgac ttttccccac
     6721 cattcgacta tcttgtttag catataaaac aaattacacc gataacagcg aatatcacgc
     6781 taatgtcggt tttaacgtta agcctgtaaa acgagatggt aagatgagcg tgatgtttga
     6841 tccagacacg gcgatttatc ctttcccccc gaagccgacg ccgttaagca ttgatgaaaa
     6901 agcgtattac cgcgagaaga taaaacgtct gctaaaagaa cgtaatgcgg tgatggttgc
     6961 ccactactat accgatcccg aaattcaaca actggcagaa gaaaccggtg gctgtatttc
     7021 tgattctctg gaaatggcgc gcttcggtgc aaagcatccc gcttctacgt tgttagtcgc
     7081 tggggtgaga tttatgggag aaaccgccaa aattctcagt ccggaaaaaa caattctgat
     7141 gccgacactt caggctgagt gttcactgga tctcggctgc cctgttgaag aatttaacgc
     7201 attttgcgat gcccatcccg atcgtactgt cgtcgtctac gccaacactt ctgctgcggt
     7261 aaaagcgcgg gcagattggg tggtaacttc aagcattgcc gtcgaactta ttgatcatct
     7321 ggatagtttg ggtgaaaaaa tcatctgggc acccgacaaa catctggggc gttacgtgca
     7381 aaaaaagacg ggcgcagaca ttttatgctg gcagggtgcc tgcattgtgc atgatgagtt
     7441 taagactcag gcgttaaccc gcttgcaaga agaatacccg gatgctgcca tactggtgca
     7501 tccagaatca ccacaagcta ttgtcgatat ggcggatgcg gtcggttcca ccagtcaact
     7561 gatcgctgct gcgaaaacat tgccacatca gaggcttatt gtggcaaccg atcggggtat
     7621 tttctacaaa atgcagcagg cggtgccaga taaagagtta ctggaagcac caaccgcggg
     7681 tgagggcgca acctgccgca gctgcgcgca ttgtccgtgg atggccatga atggcctcca
     7741 ggccatcgct gaggcattag aacaggaagg aagcaattac gaggttcatg ttgatgaaag
     7801 gctgcgagag agggcgctgg tgccgctcaa tcgtatgctg gattttgcgg ctacactacg
     7861 tggataacga ataataaggc gtaacgttac gctttggggg aaagatggat ttttttagtg
     7921 tgcagaatat cctggtacat ataccaatag gagcaggcgg ttatgatctc tcatggatcg
     7981 aagcggtagg cacaatcgcc gggttgctgt gtattggcct tgccagtctg gagaagatca
     8041 gcaactactt ctttggcctg atcaacgtca ccttgtttgg cattattttc tttcagattc
     8101 agctgtatgc cagcctgcta ttacaggtgt ttttctttgc cgcgaatatt tacggctggt
     8161 atgcgtggtc gcgacaaacc agtcagaacg aggcggagtt aaaaattcgc tggttgccat
     8221 tgccgaaggc actcagctgg ttggcggttt gcgttgtttc gattggtctg atgacggtat
     8281 ttatcaatcc ggtgtttgca tttttgaccc gcgtggcagt catcatcatg caagcattag
     8341 gattacaggt tgcgatgcct gaactacaac cggacgcttt cccgttctgg gattcttgca
     8401 tgatggtgtt atctatcgtg gcaatgattc tgatgactcg taagtatgtg gaaaactggc
     8461 tgttgtgggt gattattaac gtgattagcg tcgttatttt tgcacttcag ggcgtttacg
     8521 ccatgtctct ggagtacatc atcctgacct ttattgcgct caacggcagc cggatgtgga
     8581 tcaatagcgc acgcgaaaga ggctcacgcg cgctgtccca ttaatggtga tgatgtgaat
     8641 ggccagacac cccttcattc aggtggcagt ctggcccatg acaaggttga tattccatct
     8701 gaatggtggc gtgctcaatc tgatagtgat ccatcaggta gtgttggatc tgatccaaca
     8761 aggcatcgtg atcgtgtggc gggatcacct gcacatgcaa cgtcatcacc ggcttctcgc
     8821 ctaccatcca tacatgcaca tggtgcacat tgcggacttc cgggatttcc cggcacatac
     8881 gacgcttcag ttcagcgata tccagcgata ccggtgcacc ctcaagtaat tcattcacgc
     8941 tatctttcaa cagtcgccac gcgctacgca gaaccagaag cgacaccagt atcgagagaa
     9001 tggggtcagc aggtgtccag ccggtccaga taataatcaa cgcggcaata atagccccaa
     9061 ccgaacccag caggtccccg agcacatgca gtgccgctgc tcgtacgttg aggtttttct
     9121 cttcgctgcc gtgatgaagt aaccagaaag aaagtatatt tgccagcaac ccagccacgg
     9181 caattgccat catcatgccg ccctcgaccg gacgcggcgt gcggaagcgt tctatcgcct
     9241 cccagacaat caaaatggta attaccacga gggcgatggc attcacaaac gccgcgagcg
     9301 tggtcaatct tagccaaccg aaagtgtggc gaatggtggg aggacgacgt gaaaattgca
     9361 cggcgagtag ggcgaaaagc agggcggcag tatcggtcaa catatgaccc gcatcggcca
     9421 gcaaagccag agaaccggaa agaaaaccac caatgacttc taccagcata aacccggcag
     9481 tcacgccgaa agcatacaac aagcgacgag cattattatc ttctggcagg tgtgaggacg
     9541 tgtgtgagtg tgagtgcgcc atgacgagtc atcccttaat tattctcact taactttatg
     9601 acatcatact gcttttagaa gggaaaaatt aaaagggaga gactccgctc tcccattatt
     9661 ggctattttg cagggttact gcgtggtacc gtcggttttg gtatcgacat cattattgat
     9721 gccatcaccg gtttgtacct ttttattgat atccgggcag cgaccatctt tgcacatggt
     9781 gttcttgtgc tcttcatctt tggtcatgcc gtcattgttc attgaagaac catccggatg
     9841 cagcatagtg ccgccagaac cggtatttac cccgttattg tcgacgttat ttggcgcgac
     9901 attttcacgg gcgtcagggg ctacctggcc cgcatcagct gcggcgtttg cctggccgtt
     9961 attggtttgc gctccgctat cggcagccag tgcggcaccg ctggcaaggc ttagagtggc
    10021 agtcagaaat agtgtggcca gttttgtcat tttcatagga tgctcctgtt atggtcgtta
    10081 tgtcggataa cctcttccaa cagtgcattt gcaggtgaat ataaggcatt ggtttaagat
    10141 ttcagccagg caaagaaatc cagcagagaa tcttgaaata attaacaaac aaaggagtta
    10201 cagttagaaa ttgtaggaga gatctcgttt ttcgcgacaa tctggcgttt ttcttgctaa
    10261 ttccaggatt aatccgttca tagtgtaaaa ccccgtttac acattctgac ggaagatata
    10321 gattggaagt attgcattca ctaagataag tatggcaaca ctggaacaga catgaattat
    10381 cagaacgacg atttacgcat caaagaaatc aaagagttac ttcctcctgt cgcattgctg
    10441 gaaaaattcc ccgctactga aaatgccgcg aatacggttg cccatgcccg aaaagcgatc
    10501 cataagatcc tgaaaggtaa tgatgatcgc ctgttggtgg tgattggccc gtgctcaatt
    10561 catgatcctg tcgcggctaa agagtatgcc actcgcttgc tggcgctgcg tgaagagctg
    10621 aaagatgagc tggaaatcgt aatgcgcgtc tattttgaaa agccgcgtac tacggtgggc
    10681 tggaaagggc tgattaacga tccgcatatg gataacagct tccagatcaa cgacggtctg
    10741 cgtatagccc gtaaattgct gcttgatatt aacgacagcg gtctgccagc ggcgggtgaa
    10801 ttcctcgata tgatcactcc tcagtatctc gctgacctga tgagctgggg cgcaattggt
    10861 gcacgtacca cggaatcgca ggtgcaccgc gaactggcgt ctggtctttc ttgtccggta
    10921 ggttttaaaa atggcacaga cggtacgatt aaagtggcta tcgatgccat taatgccgcc
    10981 ggtgcgccgc actgcttcct gtccgtaact aaatgggggc attcggcgat tgtgaatacc
    11041 agcggtaacg gcgattgcca tatcattctg cgcggcggta aagagcctaa ctacagcgcg
    11101 aagcacgttg ctgaagtgaa agaagggctg aacaaagcag gtctgccagc tcaggtgatg
    11161 atcgatttca gccatgctaa ttcgtccaaa caattcaaaa agcagatgga tgtttgtgct
    11221 gacgtttgcc agcagattgc cggtggcgaa aaggccatta tcggtgtgat ggtggaaagt
    11281 catctggtgg aaggcaatca gagcctggag agcggggagc cgctggccta tggtaagagc
    11341 atcaccgatg cctgcattgg ctgggaagat accgatgctc tgttacgtca actggcgaac
    11401 gcagtgaaag cgcgtcgcgg gtaaagttta attgtcggat gcgccgtcag agtggcgtat
    11461 ccgatgaatc accgtagccc tgacaagacg cgcagcgtcg catcaggcaa tgtgctccat
    11521 tgttagcgac gacaaacaaa aaagccgact cacttgcagt cggctttctc atttttaaac
    11581 gaatgacgtt tacttcgctt taccctggtt cgcaacggct gctgctttcg ccgcgatctc
    11641 gtcagcatta cccagataat agcgtttcag cggtttgaag ttctcgtcaa actcataaac
    11701 cagcggcacg ccggtcggga tattcagctc aagaatctct tcttcgctca tgttatcgag
    11761 atatttcacc agcgcacgta acgagttacc gtgtgcagcg atgattacgc gctcaccgct
    11821 cttcatacgc ggcagaatgg tttcattcca gtaagggatc acgcggtcaa tggtcagcgc
    11881 caggctttcc gtcagcggca gttctttctc gctcagtttc gcgtaacgcg gatcgtgacc
    11941 cggataacgc tcatcatctt tagtcagttc cggcggagtc actgcaaaac cacgacgcca
    12001 ctgtttcacc tgctcgtcgc catatttttc agcagtttcc gctttgttca gaccctgcaa
    12061 cgcaccgtag tgacgttcgt tcagtttcca ggatttctca acgggcagcc atgcctgatc
    12121 cagttcgtcc agcacattcc acagggtatg gatagcgcgt ttcagcacag aggtgtaagc
    12181 aaagtcaaag ctgtaacctt cctctttcag cagcttacct gctgcttttg cttcgcttac
    12241 gcctttctca gacagatcca cgtcgtacca accggtgaaa cggttttctt tgttccactg
    12301 actttcgcca tgacgaacca gaaccagctt agttacagcc atatacttac tcctcaaatc
    12361 atcttttaat gataataatt ctcattatat tgccgtgacg aagcaacagc aatgcttacg
    12421 cataaccata gcgaaaatag tggcgcagtg taaggttgtt gtgaatattg agttgcaaat
    12481 atgtcggtgt ttgctggtga tttgaacaat atgagataaa gccctcatga cgagggctta
    12541 acattactca gcaataaact gatattccgt caggctggaa tactcttcac caggatgcag
    12601 gaaacagtcc ggttgcggcc attcagggtg gttcgggctg tccggcagaa actcgctttc
    12661 caacgccagc ccttgccagt cggcgtaagg ttcggttccc cgcgacggtg tgccgccgag
    12721 gtagttgccg gagtagaatt gcagagccgg agcggtggtg tagaccttca gctgcaattt
    12781 ttcatctgct gaccagacat gcgccgccac tttcttgcca tcgcctttgg cctgtaacaa
    12841 gaatgcgtga tcgtaacctt tcactttgcg ctgatcgtcg tcggcaagaa actcactggc
    12901 gatgattttg gcgctgcgga aatcaaaaga cgttccggcg acagatttca ggccgtcgtg
    12961 cggaataccg ccttcatcaa ccggcagata ttcgtccgcc agaatctgca acttgtgatt
    13021 gcgcacgtca gactgcttgc cgtcaagatt gaaatagacg tgattagtca tattcaccgg
    13081 gcaaggttta tcaactgtgg cgcgataagt aatggagata cggttatcgt cggtcagacg
    13141 atattgcacc gtcgcgccga gattacccgg gaagccctga tcgccatcat ctgaactcag
    13201 ggcaaacagc acctgacgat cgttctggtt cacaatctgc cagcgacgtt tgtcgaaccc
    13261 ttccgggccg ccgtgcagct ggttaacgcc ctgacttggc gaaagcgtca cggtttcacc
    13321 gtcaaaggta taacgactat tggcgatgcg gttggcataa cgaccaatag aggcacccag
    13381 aaacgcggcc tgatcctgat agcattccgg gctggcacag ccgagcagcg cctcgcggac
    13441 gctgccatcg gaaagcggaa tacgggctga aagtaaagtc gcaccccagt ccatcagcgt
    13501 gactaccatc cctgcgttgt tacgcaaagt taacagtcgg tacggctgac catcgggtgc
    13561 cagtgcggga gtttcgttca gcactgtcct gctccttgtg atggtttaca aacgtaaaaa
    13621 gtctctttaa tacctgtttt tgcttcatat tgttctgcga cagcttgctg tacggcaggc
    13681 accagctctt ccgggatcag cgcgacgata cagccgccaa atccgccgcc ggtcatgcgt
    13741 acgccacctt tgtcgccaat cacagctttg acgatttcta ccagagtgtc aatttgcggc
    13801 acggtgattt cgaaatcatc gcgcatagag gcatgagact ccgccatcaa ctcgcccata
    13861 cgtttcaggt cgccttgctc cagcgcgctg gcagcttcaa cggtgcgggc gttttcagtc
    13921 aggatatgac gcacgcgttt cgccacgatt gggtccagct catgtgcaac agcgttgaac
    13981 tcttcaatgg tgacatcgcg cagagctggc tgctggaaga aacgcgcacc ggtttcgcac
    14041 tgttcacgac gggtgttgta ttcgctgcca accagggtac gtttgaagtt actgttgatg
    14101 atgacgacag ccacaccttt cggcatggaa actgctttgg tccccagtga gcgacagtca
    14161 atcagcaagg catgatcttt cttgccgagt gcggaaatta gctgatccat gatcccgcag
    14221 ttacagccaa caaactggtt ttctgcttcc tgaccgttaa gcgcgatttg tgcgccgtcc
    14281 agcggcagat gataaagctg ctgcaatacg gttccgaccg cgacttccag tgaagcggaa
    14341 gaacttaacc cggcaccctg cggcacattg ccgctgatca ccatgtccac accgccgaag
    14401 ctgttgttac gcagttgcag atgtttcacc acgccacgaa cgtagttcgc ccattgatag
    14461 ttttcatgcg cgacaatggg cgcatcaagg gaaaactcat caagctgatt ttcataatcg
    14521 gctgccatta cgcgaacttt acggtcatcg cgtggcgcac agctgatcac ggtttgataa
    14581 tcaatcgcgc agggcagaac gaaaccgtcg ttgtagtcgg tgtgttcacc aatcaaattc
    14641 acgcggccag gcgcctgaat ggtatgagtg gcagggtagc caaatgcgtt ggcaaacaga
    14701 gattgtgttt tttctttcag actcatttct tacactccgg attcgcgaaa atggatatcg
    14761 ctgactgcgc gcaaacgctc tgctgcctgt tctgcggtca ggtctcgctg ggtttctgcc
    14821 agcatttcat aaccaaccat aaatttacgt acggtggcgg agcgcaacag aggcggataa
    14881 aagtgcgcgt gcagctgcca gtgttgattc tcttcgccat taaacggtgc gccgtgccag
    14941 cccatagagt aggggaagga gcactggaag aggttgtcat aacgactggt cagctttttc
    15001 aacgccagtg ccaaatcgct gcgctgggcg tcggtcaaat cggtgatccg caaaacgtgg
    15061 gctttgggca gaagtagcgt ttcgaacggc caggcagccc agtaaggcac aactgctaac
    15121 cagtgttcgg tttcgacaac ggtacggcta ccgtctgcca gctcgcgctg aacataatcc
    15181 accagcattg gcgatttctg ctcggcaaaa tattcttttt gcaggcggtc ttcgcgctca
    15241 gcttcgttag gcaggaagct atttgcccaa atctgtccgt gcggatgcgg gttagagcag
    15301 cccatcgccg cacctttgtt ttcaaagacc tgcacccacg ggtatgtttt ccccagttct
    15361 gcggtttgct cctgccaggt tttgacgatt tccgtcaatg ccgcaacgct cagttctggc
    15421 agcgttttac tgtgatccgg tgaaaagcag atcacccggc tggtaccgcg cgcgctctgg
    15481 caacgcatca aaggatcgtt gctttctggc gcatctggcg tgtcagacat caaggccgca
    15541 aagtcattag tgaaaacgta agtcccggtg taatcggggt ttttatcgcc tgtcacccgc
    15601 acattacctg cgcagaggaa gcaatctgga tcgtgcgcag gtaacacctg tttggctggc
    15661 gtttcctgcg ccccctgcca ggggcgctta gcgcggtgcg gtgaaaccag aatccattgc
    15721 ccggtgagct ggttgtagcg gcgatgtgga tgatcaacgg gattaaattg cgtcatggtc
    15781 gttccttaat cgggatatcc ctgtggatgg cgtgactgcc agtgccaggt gtcctgcgcc
    15841 atttcatcga gtgtgcgcgt tacgcgccag ttcagttcac ggtcggcttt gctggcgtcc
    15901 gcccagtagg ccggaaggtc gccctcgcga cgcggtgcaa aatgatagtt aaccggtttg
    15961 ccgcaggctt tgctgaaggc attaaccacg tccagcacgc tgctgcctac gccagcaccg
    16021 aggttgtaga tgtgtacgcc tggcttgttc gccagttttt ccatcgccac gacgtgaccg
    16081 tccgccagat ccattacgtg gatgtaatcg cgtacgccag taccgtcttc ggtcggataa
    16141 tcgttaccaa aaatcgccag cgagtcgcga cggcctacag caacctgggc gatgtatggc
    16201 ataaggttat tcggaatgcc ttgcggatct tcgcccatat cgcccgacgg atgtgcgcca
    16261 accgggttga agtagcgcag cagggcaatg ctccagtccg gctgggcttt ttgcagatcg
    16321 gtgaggatct gttccaccat cagcttgctt ttgccgtaag ggctttgcgg tgtgccggtc
    16381 gggaagcttt caacgtatgg aattttgggc tgatcgccat aaacggtggc ggaggagcta
    16441 aaaataaagt ttttgacgtt agcggcgcgc atggcgctaa tcaggcgcag agtaccgttg
    16501 acattgttgt cgtaatattc cagcggtttt tgtaccgatt cgccaacggc tttcagcccg
    16561 gcgaagtgga tcacggtgtc gatagcgtga tcgtgcagga tctcggtcat caacgcttcg
    16621 ttacggatat cgccttcaac aaacgtcgga tgtttgccgc ctaaacgctc gataacaggc
    16681 agtacgctgc gcttactgtt acagaggtta tcaagaatga tgacatcatg accgttttgc
    16741 agtaattgca cacaggtatg acttccaatg taaccgctac caccagtgac cagaactctc
    16801 ataattcgct ccattaggct tatggtatga aataaccata gcataacaaa gatgcgaaaa
    16861 gtgtgacatg gaataaatta gtggaatcgt ttacacaaga atttagccgt tttttatgcg
    16921 cgattaagtg attataaaac agagggattg tgaatgattg cgctttttat ctgaaaaaaa
    16981 gacgcagttt catgcctgga tgcgtctgac cgtgtgccgg ataaagcgtt tacgccgcat
    17041 ccggcacagt cagcattacc gacttagttt atttttgtca gcgcataatg atagaggcca
    17101 ccgtccggca cgaactccag acgatgggta atacaagccg gcgcatcttc agcatggtgc
    17161 gaaacaaaca acaattgcgt ttcaccttcg ctaatcagca catcaacaaa acggcgaata
    17221 agctggcgat tgagcggatc aagcccctgc aatggttcgt cgagaataag caacgtcgga
    17281 tgtttcacca gtgcgcggac gatcagcgcc agacgctgct gtccccagga aagactatgg
    17341 aacggagcgt cagccgtgcg tttatcaatg ccgagaatat ccagccactg ctgcaccagt
    17401 ttttgctggc gatccgaaac ggcctgataa atgccaatcg aatcaaaata gccagaaaga
    17461 atcacattac gcacagtagt actgacccgg taatccagat gcaaactact gctgacgtaa
    17521 ccgatatgct ttttgatatc ccagatggtt tcgccgctgc cacgacgtcg tccgaaaagc
    17581 gtcaaatcgt tgctgtaacc ttgcggatga tcgccagtaa tcaggcttaa taacgtcgat
    17641 tttcccgcgc catttggccc gacaatttgc cagtgttcac ctggattcac ctgccagcta
    17701 aggttattaa gaatggggcg atcgttataa gaaaccacgc cattattcag cacaatgcgc
    17761 ggttcgttgg cgggtaaggc gtgacgtgct gaaggttcat ccggctccgg cagttgcaca
    17821 ccttcaagct gttcactatg cgccagttgc gcgacgagtg cttgttggag cagttcctct
    17881 ttagcgccag tttccgctaa cgtgcaatcc gccagcacgc cagcaaactg gacaaactcc
    17941 gggatctcat cgaagcgatt gagcaccagt accagagtaa taccggactg atgtaacgag
    18001 gcgagtcgct cagccagctg ctgacgtgag gcaacatcca ggccatcgaa cggctcatca
    18061 agaatcaaca agtcaggctc cgacatcagc gcctgacaca gcagggtttt tcgcgtctcg
    18121 ccagtggaaa ggtatttaaa gcgtcggtcg aggagggggg taataccgaa ctgctgtgcc
    18181 agttgcatgc agcgcggtgc atcctttact tcatcctgaa taatctcagc cgtagtgcgt
    18241 ccggtgtcat cttcgccagg gccgagcata tcggtgttat tccgctgcca ttcgtcgctg
    18301 acgagctttt gcaattgctc gaaggagaga cgagtgatgt gggaaaactg gctttgccgt
    18361 tcacctttca aaagcggaag ttcccccgcc agcgcgcggg ccagggccga tttcccgctt
    18421 ccattcgaac cgacaaacgc ccaactatca cccgcgttta acgttagctg aggcaattgc
    18481 agcgtttttg tgtcgctaag acgaaacgtg ccttgcaaaa tttgcaacga tgacatttta
    18541 tatcccattt tgtgcagcga ttactgacag ggatacgtgt ttcataacaa attgtcaaca
    18601 cgcttagcac agtgtggcga taatcacgct gtcggcatta aagtaggccg tgacattctg
    18661 tccttgctga agagaagtcg cttcatttac cggcactgtg gcgcacagtg tttgcccgtc
    18721 cggtagcgcc attaatactt cgcactgctc tgcgccgcgc tcaatatgac taataatacc
    18781 cggtaattgg ttgtcagcgt tttgcgcgac cgcttcgtcc tgagtaatgc ctacccacgg
    18841 cgcttttagc aatatcaaca cttctttgcc ttcatccagc cccagacgcg cgccgctttg
    18901 tgcggtaatt gcgactttca ggcgtgtttt tccatcagcc agtaagacat caacatgctg
    18961 ttgaacgtca tcatgatcgc gggcggtgat ggtaccgaac cactggttac gggcgctggt
    19021 ttggagtgaa aaacgtgaga tcgcggccag caggctgttc agcggcaggg cgtcatcgtc
    19081 acttaacaca tcaaaggctt tttgctggat ttgcgccagt aagtcataga gctgaatcag
    19141 tcgctgacca tagcgggtca gtactgcgcc gccgccacct ttaccgcctg ttgcgcgctc
    19201 gaccagaata tgctcactta actgattcat ctcgttaatg gcatcccagg cgcttttata
    19261 gctgataccg gcatctttcg ctccctggct aatggaaccg gaaagcgcaa tgtgttttag
    19321 tagcgaaatg cggcgcgggt cggcgaataa tttttgttgg agcttaaggg tgagaaggat
    19381 ttcggcctgc ataacaatgt cctggcaaaa gtcttattgt gacggaaaac gaacgccacg
    19441 caaagctgac cgcacaaaag gggagtgctt ttctgtgctt agcggttaga atagtctcat
    19501 gactatatct ggagttgacc atgttagagt tattaaaaag tctggtattc gccgtaatca
    19561 tggtacctgt cgtgatggcc atcatcctgg gtctgattta cggtcttggt gaagtattca
    19621 acatcttttc tggtgttggt aaaaaagacc agcccggaca aaatcattga ttccctgaat
    19681 gcccgcttag tcgggcattt tctttttctc aacttcctgc ttttcctgcc gatatttttt
    19741 cttatctacc tcacaaaggt tagcaataac tgctgggaaa attccgagtt agtcgttata
    19801 ttgtcgccta cataacgtta cattaagggg ttaccaatgg ctcgtaaatg gttgaacttg
    19861 tttgctgggg cggcactctc tttcgctgtt gctggcaatg cactggcaga tgaagggaaa
    19921 atcacggtgt tcgccgccgc atcactgact aacgcaatgc aggacattgc tacgcagtat
    19981 aaaaaagaga aaggcgtgga tgtggtttct tctttcgctt cgtcatctac tctcgcccgt
    20041 cagattgaag cgggtgcctc tgcggatctg tttatttctg ccgatcagaa atggatggat
    20101 tatgcggttg ataaaaaagc gatcgataca gctacgcgtc agacactgct cggcaatagc
    20161 ctggtcgttg tagcaccgaa agccagcgag cagaaagatt ttaccatcga cagcaaaacc
    20221 aactggactt cactgctgaa tggcggtcgc ctggcggttg gcgatccgga acatgttccc
    20281 gctggcattt atgcaaaaga agcactgcaa aaactgggcg catgggatac gctctccccg
    20341 aaactggctc cggcggaaga tgttcgtggc gcgctggcgt tggtcgaacg caatgaagcg
    20401 ccgttgggca ttgtttacgg ttctgacgca gttgctagca aaggggtaaa agtggttgcc
    20461 accttcccgg aagattcaca taaaaaagtg gaatatccgg ttgctgttgt ggaagggcat
    20521 aacaatgcga cagtgaaagc attttatgat tatctgaagg gaccgcaggc agcggaaatc
    20581 tttaaacgtt acggatttac aaccaagtaa tgatactgac cgatccagaa tggcaggcag
    20641 ttttattaag cctgaaagtt tcttccctgg ctgtgctgtt tagcctgccg tttgggatct
    20701 tttttgcctg gttactggtg cgttgcacgt ttccgggcaa agctctgctc gacagcgtac
    20761 tgcatctacc gctggtgtta ccgcccgtgg tcgtcggtta cttattatta gtttcgatgg
    20821 ggcggcgcgg atttatcggt gaacgtctgt atgactggtt tggtattacc ttcgccttta
    20881 gctggcgcgg cgcggttctc gctgccgccg tcatgtcgtt tccgctgatg gtgcgggcaa
    20941 ttcgtctggc gctggaaggg gttgatgtca aactggaaca ggccgcaaga acactggggg
    21001 ccgggcgctg gcgcgttttc tttactatca cgttaccgct gaccttaccg ggaattattg
    21061 ttggtacggt actggctttt gctcgttctc tcggtgaatt tggcgcaacc atcacctttg
    21121 tgtcgaacat tcctggtgaa acgcgaacca ttccttctgc catgtatacc ctgattcaga
    21181 cccccggcgg ggaaagtggc gcagcgagac tgtgcattat ttctattgcg ctggcgatga
    21241 tctccctgtt gatttcagaa tggctggcca gaatcagccg tgaacgggcg gggcgctaat
    21301 catgctggaa ctgaattttt cccagacgtt gggcaaccat tgcctgacta ttaatgaaac
    21361 gctgcccgcc aatggcatca ctgctatctt tggcgtctcc ggtgccggaa aaacttcgct
    21421 gattaacgcc atcagtggac tgacgcgccc gcaaaaaggg aggattgtcc tcaatggtcg
    21481 ggtactaaat gatgccgaaa aaggtatctg cctgacgccg gaaaagcgtc gcgttggcta
    21541 tgtttttcag gatgcgcggc tgttcccgca ttacaaagtg cgtggcaatc tgcgctacgg
    21601 catggcgaaa agcatggtcg atcagttcga taagctggtg gcgcttttag gcattgaacc
    21661 gttgcttgac cgtttaccag gcagcctgtc cggaggcgaa aaacagcgcg tagcgattgg
    21721 gcgggctttg ctgacggcac cagaattgct gttgctggat gaaccgctgg cgtcactgga
    21781 tattccgcgt aaacgcgaac tgttgcctta tctgcaacgt ctgacgcggg aaatcaacat
    21841 cccgatgttg tatgtcagcc attcgctgga tgagatcctc catctggcag acagagtgat
    21901 ggtactggaa aacggtcagg tgaaagcctt tggcgcgctg gaagaagtct ggggtagtag
    21961 cgtgatgaat ccgtggctgc cgaaagagca acaaagtagc attctgaaag tgacggtact
    22021 tgagcatcat ccgcattacg cgatgaccgc gctggcgctg ggcgatcagc atttgtgggt
    22081 caataagctg gacgaaccgc tgcaagctgc gctacgtatc cgcattcagg cttccgatgt
    22141 ttctctggtt ttacaaccgc cgcagcaaac cagcattcgt aacgtattgc gggcaaaagt
    22201 tgttaatagt tatgacgaca acggccaggt ggaagtggaa ctggaagtcg gcggtaaaac
    22261 gctgtgggcg cgtatcagcc cgtgggccag ggatgaactg gcgatcaaac ctggcctgtg
    22321 gctgtacgcg caaattaaaa gtgtgtcgat aaccgcctga ttaaatcagg tggctataaa
    22381 tgaactgggc aatgctgtcg gtggtgttat caccaatcac aatgttggcg cgcgctttta
    22441 ccgcgtcatc ggcgttgccc atcgccacgc ctgtaccagc ggcctccagc atactgatat
    22501 cattaaagtt atcgccgaac gccacgacgt tttccatcga ccagccttgc gcctcaaccc
    22561 atttcgtcaa acgtttacct ttgctgttgc cgccgcgtgc aatatcaacc tgatcgtgcc
    22621 aggaccattc acactccagt cccagttcat gttcgacatg cttaccaaaa tgctgcaatt
    22681 gcggcaggtc atcgtgcgtg agggcgaact tccatacggc gttaacttgt tgcgccgttt
    22741 cagccagaga agcgacttgt gtgaaagtcg gacgctgttc cggcggcagg gtttgcgccc
    22801 agttagacgt gcgaatgaca tgcccggtcg ggtgctcata aaccatctcg tcatcgacat
    22861 acatcagacc gtgaatgtgg tgttcattca gcatctcaat gagttgtagg gctttattaa
    22921 cgggcattgg gtccgcttcc agcacggttt ttgcatgata atcatacaaa taggtgccat
    22981 tacagcaaat agcaggtgta tccagcgcca gcgcctgata aaaaggatga atagcgacgt
    23041 gatggcgacc tgtgacgatg attaattgat aacctgcttc gcgagcgcgg gccagggctt
    23101 ctatcgatga aggtagcagg gtctttttcg gggtcaataa ggtgccgtct aagtcgagag
    23161 caatcacgcg tgtggtcatg gcgtattcca gattaaggtt aagaattttc ttctgcgcga
    23221 atggtacacc gataccacta tcggacaaaa ttctgcgttt taattcagca ttcaccgcca
    23281 aaagcgacta attttagctg ttacagtcag ttgctaaatg caaaggagca ttcatgaagc
    23341 aaacagttta tatcgccagc cctgagagcc agcaaattca cgtctggaac ctgaatcatg
    23401 aaggcgcact gacgctgaca caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg
    23461 tcagcccgga caaacgttat ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt
    23521 atcgtatcgc cccggacgat ggcgcactga cctttgccgc agagtctgcg ctgccgggta
    23581 gtccgacgca tatttccacc gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg
    23641 cgggtaacgt gagcgtaacg cgtctggaag atggcctgcc agttggcgtc gtcgatgtgg
    23701 tcgaggggct ggacggttgc cattccgcca atatctcacc ggacaaccgt acgctgtggg
    23761 ttccggcatt aaagcaggat cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg
    23821 tggcgcagga ccctgcggaa gtgactaccg ttgaaggggc cggcccgcgt catatggtat
    23881 tccatccaaa cgaacaatat gcatattgcg tcaatgagtt aaacagctca gtggatgtct
    23941 gggaactgaa agatccgcac ggcaatatcg aatgtgtcca gacgctggat atgatgccgg
    24001 aaaacttctc cgacacccgt tgggcggctg atattcatat taccccggat ggtcgtcact
    24061 tatatgcctg tgaccgtacc gccagcctga ttactgtctt cagcgtttcg gaagatggca
    24121 gcgtgttgag taaagagggc ttccagccaa ctgaaaccca gccgcgcggc tttaatatcg
    24181 atcaccgtgg taagtatctg attgccgccg ggcaaaaatc gcaccacatc tcggtatacg
    24241 aaattgttgg tgagcagggg ctactgcatg aaaaaggccg ctacgcggtc gggcagggac
    24301 caatgtgggt ggtggttaac gcacactaac cgctgattta cccggcgcag tctctcctgc
    24361 gccggtgtat taacctatct cctgtaacgc atgtctctgg cgttcgacga tattggtcca
    24421 caaattgtct ttatcgtcag tccacaaatt aatcagcaag gcaaaaaagc gttctgctgc
    24481 cggagaaagc acggcatctt tgcggcgaat aatccccaat gtccgacgta taacgggttc
    24541 caccagtggg ataccaataa tagaagaata gggcgcatgg ggcattgcca gaccaggaag
    24601 cgccgaaatt cccagtcccg cctctaccag tcctaatgac gtcgaaagat gacgcacttc
    24661 gtaaaaccag tccagcttcc agggcttgtc ggctagctgt tgttctatca acaatcggtt
    24721 gccactggag gaacgtacgc caatcatttt gtaacccact aactcctgcc attctacaag
    24781 ctgcttttta gccaatggat ggtcacgccg acaggccagc acgaacggct cgttgaccag
    24841 tggggtaaaa tcaatggatg aatttgtgac gttgttcatg tttatgccaa agtctgattc
    24901 gttacacagc accgattcca tgcaattatt cgtgccttgt tccagaatcc gcactttaat
    24961 attgggatac agctcattaa atttaccgat tgccagcggt aaaaaataaa atactgcggt
    25021 cggaatacac gctaacgtca ccataccacg atgataagca ttcatatcac gaatattaaa
    25081 aagcgtttca tcgaatttct ttattaattc ccgcgcctct gggagcaacc tttttcctgc
    25141 ttttgttaac gtaaccttgc gagttgtacg ctcaaatagt tgaatatgta aatcttcctc
    25201 catctttttt attcttcgtg ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt
    25261 gttaaatgaa ctggactctg ccagtatgac aaatgccttc atacttgata attcatgctt
    25321 cattattact ccgggaaatg gaagcgacga ttttgggtgg ctggccgtta aaaattttaa
    25381 ctgcatttag ccaacttaaa ttaatgaaaa aatgttatta atcgttgagc taaagtcatt
    25441 agagatgctt tgcccttaat gtaaccatat cgcaataagt tatgttttta aattgagggc
    25501 attattatga aaaaaatacc ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc
    25561 ctgttagcgg aacatttacc cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt
    25621 atgggttccg gtaacgatct ggaaattgac ggtattggcg gcggtaatcc actgaccagt
    25681 aaagtcgcca ttattagccg ttccagcgat ccgcgtgctg atgttgatta tctgtttgct
    25741 caagttatcg tccatgagaa acgtgtcgat accacgccta actgcggcaa tatgctgtct
    25801 ggcgttgggg cattcgccat tgaaaatggt ttgattgcag cgacttcgcc agttacccgc
    25861 gtacgtatcc gcaacgtcaa tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat
    25921 ggtgttgtcg agtacgaggg tagcgccaga attgacggcg taccgggtac tgccgcaccg
    25981 gttgcgctca ctttcctgaa tgccgctgga acgaaaaccg gaaaagtttt cccgactgat
    26041 aatcagattg attattttga cgatgtcccg gtgacctgta tcgatatggc gatgccagtc
    26101 gtcattattc cggctgaata tctgggaaaa acaggctatg aattaccggc ggaactggat
    26161 gccgacaaag cattattagc ccgcattgaa tctatccgtc tacaagcggg taaagcaatg
    26221 ggcttaggcg atgtcagtaa tatggttatc cctaaacctg tgcttatttc tccagcgcag
    26281 aaaggcgggg caattaatgt gcgttatttt atgccgcatt cttgccatcg cgcgctggcg
    26341 ataaccggtg ctattgctat ttccagtagt tgtgcattgg aaggtaccgt cacccgacaa
    26401 atcgtccctt ctgtaggata cggcaatatc aatattgaac accccagtgg tgggctcgac
    26461 gttcatttaa gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt tattcggaca
    26521 accagaaaaa tattttccgg tgaagtttat cttccctgaa aagattcgtt gtcaggataa
    26581 ggacaatcaa taaaggactt ctgtatgagt catacagaaa gaacaggatt ttaaatgaat
    26641 aagaaatcgt tatggaagct aattctgata ttagcgatcc catgtattat tggctttatg
    26701 ccagctccgg caggattaag cgaactggcg tgggtgcttt ttggtattta cctggcggcc
    26761 attgtggggc tggttatcaa gcctttcccg gaacctgtcg tactgttaat tgccgttgct
    26821 gcctccatgg tggtggtcgg taacttatcc gacggtgcgt ttaagaccac cgccgtatta
    26881 agcggttact cttcaggtac tacctggctg gtgttctcgg cgtttacctt aagcgccgca
    26941 tttgtgacca ccggtttagg taaacgtatt gcctatctgc tgattggtaa aatcggtaac
    27001 accacgctgg gtctgggtta cgttacggta ttcctcgatc tggtattggc tccggcaaca
    27061 ccgtctaaca ccgcgcgtgc gggcggcatt gtgttaccga tcatcaacag cgtggcggtg
    27121 gctttggggt ccgaaccgga aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc
    27181 atttacatgg tcaccaaaac caccagctat atgttcttta ccgcaatggc ggggaacatt
    27241 ctggcgctga aaatgatcaa cgacattctg cacctgcaaa ttagctgggg tggatgggcg
    27301 ctggccgccg gattgccggg catcattatg ctgctggtca ccccgctggt gatttacacc
    27361 atgtatccac cagaaattaa gaaggtggat aacaaaacca tcgctaaagc gggccttgcc
    27421 gaactgggac cgatgaaaat ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgctg
    27481 ctgggctgga ttttcagtaa gtctctgggg gttgatgaat cttcagtggc tattgttgtt
    27541 atggcgacca tgctgctgct gggtatcgtt acctgggaag acgtggttaa aaataaaggc
    27601 ggctggaata ccttaatctg gtacggcggt attatcggct taagctcctt attatcgaaa
    27661 gttaaatttt tcgaatggtt agctgaagtc tttaaaaata acctggcatt tgatggtcac
    27721 ggtaacgttg ctttcttcgt tattattttc ctcagcatca tcgtgcgtta tttcttcgct
    27781 tccggtagtg cctatatcgt tgctatgtta ccggtatttg ccatgctggc gaacgtctcc
    27841 ggcgcaccgt taatgttaac cgcgctggca ctgttgttct ccaactccta tggcggcatg
    27901 gttactcact atggcggcgc ggcaggtccg gtcatctttg gcgtgggtta taacgatatt
    27961 aaatcctggt ggttggtcgg tgcggtactg acgatattaa ccttcctggt gcatatcacc
    28021 ctcggcgtgt ggtggtggaa tatgctgatc ggctggaaca tgctgtaaat atactcgtca
    28081 tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag
    28141 ctcctgggga ttcactcgct tgtcgccttc ctgcaactcg aattatttag agtatatcca
    28201 tttattttct tgctgcgcac ttcacggtgc gcagatatct ggagcatttg atgatcaagt
    28261 tatctgaaaa aggcgtgttt ctcgccagta ataacgaaat aattgccgaa gaacatttca
    28321 ccggcgaaat taaaaaagaa gaagcaaaaa aaggcactat tgcctggtct attctctctt
    28381 ctcataatac gtccggaaat atggataaac ttaaaattaa gtttgattca ttagcctctc
    28441 acgatattac ctttgttggt attgtacaga ccgctaaagc gtccggtatg gaacgtttcc
    28501 cgctgccgta tgtgctgacc aactgccata actcactctg cgccgtcggc ggcaccatta
    28561 acggtgatga ccatgttttt ggtttatcgg cagctcagcg ttatggcggt atttttgtgc
    28621 ctccgcatat tgcggtcatc catcaatata tgcgtgagat gatggcaggt ggcggcaaaa
    28681 tgatcctcgg gtcagacagt cacacccgtt acggtgcatt agggacaatg gcagtcggtg
    28741 agggcggcgg cgagttggta aaacaattgc ttaatgacac ctgggatatc gactatccgg
    28801 gagttgttgc ggtgcatctg accggaaaac cagcgccgta tgtggggccg caggatgtgg
    28861 cgttggctat catcggtgcc gtgttcaaaa acggctacgt caaaaacaaa gtgatggaat
    28921 tcgtaggtcc cggtgttgct gcgctctcta ccgatttccg taacagcgtt gacgttatga
    28981 ccactgaaac gacctgttta agttctgtct ggcaaaccga tgaagaagtc cataactggc
    29041 tggcgctgca cggtcgcggc caggattact gccagcttaa ccctcaaccg atggcgtact
    29101 acgatggctg catcagcgtt gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc
    29161 acccgagcaa cgtgtatgaa atcgacacac tgaaccagaa cttgaccgac attctgcgtg
    29221 agattgaaat tgagtccgaa cgcgtggcgc acggtaaagc caaactctcg ctgctggaca
    29281 aagtggaaaa tggtcgcctg aaagtgcagc aggggattat cgcgggctgt tctggcggta
    29341 actacgaaaa cgtcatcgcg gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca
    29401 ccttctcgct ggcggtttac ccgtcatcac agccggtgtt tatggatctc gccaaaaaag
    29461 gtgtggtagc agatttgatt ggcgcaggcg caatcatcag aaccgcgttc tgcggcccat
    29521 gctttggcgc gggcgatacg ccaatcaaca acggtttgag tattcgccac accacgcgta
    29581 acttcccgaa ccgcgaaggc tctaagccag ctaatgggca gatgtcagcg gtggcgttga
    29641 tggacgctcg ttctatcgct gcgactgcgg caaacggtgg ctatttaacc tctgccagcg
    29701 aactcgattg ctgggacaac gtgccggagt acgccttcga tgtaacgccg tataaaaatc
    29761 gcgtctatca aggctttgtg aaaggggcgg cccagcaacc gctgatttac ggaccgaaca
    29821 ttaaagactg gccggaattg ggtgcgctga ctgacaatat cgtcctcaaa gtgtgctcga
    29881 agatcctcga cgaagtgacc accaccgacg aattgattcc ttccggtgaa acctcttctt
    29941 atcgttcaaa tccgattggt ctggcggagt ttaccctgtc acgccgcgat ccaggttatg
    30001 ttggcagaag taaagcgact gctgagctgg aaaatcagcg tctggcgggg aatgtcagcg
    30061 agctgacaga ggtgtttgcg cgcattaagc agattgctgg tcaggagcat attgatccgc
    30121 tgcaaactga aattggcagc atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg
    30181 aacaggcggc gagttgtcag cgtgtgattg gcggtctggc gaatattgcc gaggagtacg
    30241 cgactaaacg ctatcgttct aacgtcatca actgggggat gttaccgctg cagatggtgg
    30301 aagcgccaac ctttgaagtg ggggattaca tttacatccc tggcattaaa gcggcgctgg
    30361 ataatccggg tacgacgttt aaaggttatg tgatccatga agatgcgccg gtaacggaaa
    30421 ttacgctcta tatggaaagt ctgactgctg aagagcgcga gattatcaag gcgggtagtt
    30481 tgattaactt caataaaaac cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt
    30541 cggcacttat tgtcggatgc gatgctggcg catcttatcc gacctacaaa tcgcatcgaa
    30601 ccgtaggccg gataaggcgt ttacgccgca tccggcaaat agttaatagc tcttacttct
    30661 tcgcctctgc aaccactttg ctacccacgc cgcggttatt gtattcccac atgcggttgt
    30721 agttagtgtc attcagattg cgctgtattt cgtcgctatc gtcaacgctg ccggtgttac
    30781 ccgcaaacgg acgattagag atcaccgcat cgccccacgg tttagccgtg ttaaaacctt
    30841 cgttgatggc gctatcacgg atcaccacct gaccgttggt attggcatca acatccagcg
    30901 agcggcccag ttgtgccacg ccatcaccgg aagcattgaa acggctgttt acggcgagga
    30961 aaccgtagta aatgttggac agcgtagccg gtgcaaacac atacgcttct tgctgagtac
    31021 gtgagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga ccagaaacga
    31081 tatccacatc cccttcaatg tagctgttgg tcaccagcgt acgcggctga cgattcgttt
    31141 ccagacggtt ctgcacaccg ctgttggtga caaagaaggt gttctgacga ccgagaatgt
    31201 taacgttgtt aatctgtacc tggtcaccgt cagtacgcag tgccaccgcc ggatggttac
    31261 ccgcatctac gctatcaccc agcgtgtttt cgatggtcag gttttgcagt tgcaggccat
    31321 tgttttgtga ccagaagacc gcagaacaca gaacaccgat actgtcgctg cgtttgctct
    31381 ggcagctatc gtacatatac cacgctggtt tacctggcat atatttgccg cgcgggttga
    31441 cgtcgtgacg ccagtcggca gggctcatgc ccccatcaag ggaaagccca atcttcacat
    31501 caatcggttt ttcacctgta ccgtacagag taattccacc tggagcagca gggacataca
    31561 ccgttccctg atactcacca ggcatcacgg caatatactg gcgcttgttg gtgcgcttga
    31621 taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga gtacccgccg
    31681 ggccgacaac aaagtcaggt tgcgcaggca gggttatcgg ggacggattc cacgctgcag
    31741 cacctggtgt cagggatgca aaatagtgtt gagcatcgaa attctgcgct tcttttgccg
    31801 acagaatcgg gcgagaagag gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg
    31861 gtgttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa
    31921 ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat ccgttgaagt
    31981 ctgctttttt atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg
    32041 aacaggtcac tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactccct
    32101 gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttatgcccga gaagatgttg
    32161 agcaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc gcaactcgtg
    32221 aaaggtaggc ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc gcataaaata
    32281 ccttgatact gtgccggatg aaagcggttc acgacgagta gatgcaatta tggtttctcc
    32341 gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatatcccga gagcatcaac
    32401 atgcaatgtt gtagggatgg caatttttac gcctgttttg ctttgttcga cataaagata
    32461 tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgct gcccggtaac
    32521 aacagccagt tccattgcaa gtctgagcca acatggtgat gattctgctg cttgataaat
    32581 tttcaggtat tcgtcagccg taagtcttga tctccttacc tctgattttg ctgcgcgagt
    32641 ggcagcgacc gggtttgttg ttatatggcc ttcagctatt gcctctcgga atgcatcgct
    32701 cagtgttgat ctgattaact tggctgacgc cgccttgccc tcgtctatgt atccattgag
    32761 cattgccgca atttcttttg tggtgatgtc ttcaagtgga gcatcaggca gccccctcct
    32821 tattgcttta attttgctca tgtaatttat gagtgtcttc tgcttgattc ctctgctggc
    32881 gaggattttt tcgtagcgat caagccatga atgtaacgta acagaattat cactgttgat
    32941 tctcgctgtc agaggcttgt gtttgtgtcc tgaaaataac tcaatattgg cctgtattgc
    33001 ttcagtgatt gctatcctcc tgtctcggcc taaaccaaac tctttacccg tccttgggtc
    33061 cctgtagcag taatatccat tgtttcttat ataaaggtta gggggtaaat cccggcgctc
    33121 atgacttcgc cttcttccca tttctgatcc tcttcaaaag gctacctgtt actggtcgat
    33181 ttaagtcaac ctttaccgct gattcgtgga acagatactc tcttccatcc ttaaccggag
    33241 gagggaatat cctgcattcg cgcacccatc gacgaactgt ttcaaggctt cttgggcgtc
    33301 gctggcgtgc gttccactcc tgaagtgtca agtacatcgc aaagtctccg caattacacg
    33361 caagaaaaag ccgccatcag gcggcttggt gttctttcag ttcttcaatt cgaatattgg
    33421 ttatgtctgc atgtgctatc tgcgcccata tcatccagtg gtcatagcag tcattgatgt
    33481 tctctgcttc gataactctg ttgaatggtt ctccattcca ttcacctgtg actcggaagt
    33541 gcatttatca tctccataaa acaaaacccg ccgtagcgag ttcagataaa agaaatcctc
    33601 gtcagtgcga ggatgctgtt cattgctgct atacactttt ttgctctcaa cgtaagcggt
    33661 agctcattct gttgggttgg tgcagttgct tttaggaaat gctatttacc ccttaaacgt
    33721 cggctgaaag agctaaaatc catgcaaaaa atttacgcaa ttttgtgtat tattgtgcag
    33781 taagtaatga gctattttct gcgcaaaaaa tggatggtaa atttgtccgg ggcaggaaaa
    33841 attttatggg cgctaaacat gaaaaaagat tcgtatcctt atttgatttg catgacagtt
    33901 ttaggcctga tctttatttt ccttttcttc tggtggcggg cagatattta cagggtcacg
    33961 tttcttaatc agagtatatc ccactattac attctgttta gcatgggaat agcttttctg
    34021 ttatctctgt tttgggttaa gaaggggata gtaaaacaaa gcggctggaa gagtctgtca
    34081 gcatacctta aggtttatgc agggatgtgc atatttgctg gattttttct gattataccc
    34141 cttacaacac taacttattt tttgcctgga gagacatcgt cttatgttgc accgtatcgg
    34201 tatacttccg gtagttcaaa aagttgttct ggagctgagg tggatgaccc cgatctacat
    34261 gagaatattc gcatttgcta tccgtatggc aattatgagt acgataatat tatctatgtt
    34321 gaaaagaaaa ttaatacatt aggtgcggta gtaacatatg cacagaccgc gcgtgatgat
    34381 actgaatgag atagtatata gcgggcaagt tttagttaat ttatcgaggt aatataattt
    34441 acctcgactc gtttattctg gtattaatat ttcgctttac gatcgatttt tatctgatga
    34501 tatcatgcgg ttttcatata ctgacttact gtcttttctc cgttagcgat tttctcctgc
    34561 tcagcgatga ttttatcttt ggcttctagt taatttcgct cacttcgaac ctctctgttt
    34621 actgataagt tccagatcct cctggcaact tgcacaagtc cgacaaccct gaacggccag
    34681 gcgtcttcgc tcatctatgg gatcgccaca ctcacaacaa tgagtggcag atatagcctg
    34741 gtggttcagg cggcgcattt ttattgctgt gttgcgctgt aattcttcaa tttctgatgc
    34801 tgaatcaatg atgtctgcca tcttccatta atccctgaat tgttggttaa tacgcttgag
    34861 ggtgaatgcg aataataaaa aaggagcctg tagctccctg atgattttgc ttttcatgtt
    34921 caccgttcct taaagacgcc gtttaacatg ccgattgcca gacttaagtg agtcggtgtg
    34981 aatcccatca gcgttaccgt ttcgcggtgc ttcttcagta cgctacggca aatgtcatcg
    35041 acgtttttat ccggaaacgg ctgtctggct tttttgattt cagaattagc ctgacgggca
    35101 atactgcgaa gggcgttttc ttgctgaggt gtcattgaac aagtcccatg tcggcaagca
    35161 taagcacaca gaatatgaag cccgctgcca gaaaaatgca ttcagtggtt gtcataccag
    35221 gtctctctca tctgcttctg ctttcgccac catcatttcc agcttttgcg aaagggatgt
    35281 ggctaacgta tgaaattctt cgtctgtttc tactggtatt ggcacaaacc tgactccaat
    35341 ttgagcaagg ctatgtgcca tctcaatact cgttcttaac tcaacaggag atgctttgtg
    35401 catatcgcct cccgtttatt atttatctcc tcagccagcc gctgggcttt cagcggattt
    35461 cggataacag aaaggccggg aaatacccag cctcgcttcg taacggagta gacgaaagtg
    35521 atcgtgccta cgcggatatt atcgtgagga tgcttcatcg ccattgctcc ccaaatacaa
    35581 aaccaatttc agccagtgcc tcgtccattt tttcgatgaa ctccggcacc atctcgtcaa
    35641 aacccgccat gtacttttca tcccgctcaa ccacgacata atgcaggcct tcacgcttca
    35701 tacgcgggtc atagttggca aagtaccagg catcttttcg cgtcacccac atgctgtact
    35761 gcacctgggc catgtaagcc gactttatgg cctcgaaacc accgagcctg aacttcatga
    35821 aatcccggga ggtaaacggg catttcagct caaggccgtt gccgtcactg cataaaccat
    35881 cgggagagca ggcggtgcgc atactttcgt cgcgatagat gatcggggat tcagtaacat
    35941 tcacgccgga agtgaattca aacagggttc tggcgtcgtt ctcgtactgt tttccccagg
    36001 ccagcgcctt agcattaact tccggagcca caccggtgca aacctcagcc agcagggtgt
    36061 ggaagtagga cattttcatg tcaggccact tctttcctga gcggggcttt gctatcacgt
    36121 tgtgaacttc tgaagcggtg atgacgccga gccgtaattt gtgccatgca tcatccccct
    36181 gttcgacagc tctcacgtcg atcccggtac gctgcaggat aatgtccggt gtcatgctgc
    36241 caccttctgc tcagtggctt tctgtttcag gaatccaaga gctttcactg cttcggcctg
    36301 tgtcagttct gacgatgcgc gaatgtcgcg gcgaaatatc tgggaacaga gcggcaataa
    36361 gtcgtcatcc catgttttat ccagggcgat tagcagagtg ttaatctcct gcatggtttc
    36421 atcgttaacc ggagtgatgt cgcgttccgg ctggcgttct gcagtgtatg cagtattttc
    36481 gacaatgcgc tcggcttcat ccttgtcata gataccagca aatccgaagg ccagacgggc
    36541 acactgaatc atagctttat gccgtaacat ccgtttggga tgcgactgcc acggccccgt
    36601 gatttctctg ccttcgcggg ttttgaatgg ttcgcggcgg cattcatcca tccattcggt
    36661 aacgcagatc ggatgattac ggtccttgcg gtaaatccgg catgtacagg attcattgtc
    36721 ctgctcaaag tccatgccat caaactgctg gttttcattg atgatgcggg accagccatc
    36781 aacgcccacc accggaacga tgccgttctg cttatcaggg aaggcgtaaa tttctttcgt
    36841 ccacggatta aggccgtact ggttggcgac gatcaacaat gcgatgaact gcgcatcgct
    36901 ggcatgaaag cgcgatgggg agacagggcg gcatgagacg acagcgacga agtttcaccg
    36961 acatcatctg cgaaaactgc aaataccttc caacgaaacg ctccagaaat aaacgcaagc
    37021 caatcccaaa agaatctgac gtaaaaacct tcaactacac ggctcacctg tgggatatcc
    37081 ggtggctaag acattgtgcg aggaaaacaa ggtgattgac caaaatcgaa gttacgaaca
    37141 agaaagcgtc gagcgggctt taacgtgcgc taactgcggt cagaagctgc atgtgctgga
    37201 agttcacgtg tgtgagcact gctgcgcaga gctgatgagc gattcgaata gctcgatgca
    37261 cgaggaaaaa gatgatggct aaaccagcgc gaagacgatg taaaaacgat gaatgtcggg
    37321 aatggtttca ccctgcattc gctaatcagt ggtggtgctc tccagagtgt ggaaccaaga
    37381 tagcactcga acgacgaagc aaagaacgcg aaaaagcgga aaaggcagaa aaggcagcag
    37441 agaagaaacg acgacgagag gagcagaaac agaaagataa acttaagatt caaaaactcg
    37501 ccttaaagcc ccgcagttac tggattaaac aagcccaaca agccgtacac gccttcatca
    37561 gagaaagaga ccgcgactta ccatgtatct cgtgcggaac gctcacgtct gctcagtggg
    37621 atgccggaca ttaccggaca actgctgcgg cacctcaact ccgatttgat gaacgcaata
    37681 ttcacaagca atgcgtggtg tgcaaccagc acaaaagcgg aaatctcgtt ccgtatcgcg
    37741 tcgaactgat tagccgcatc gggcaggaag cagtagagga aatcgaatca aaccataacc
    37801 gc
//
LOCUS       DAZKFZ010000022        37230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000022 DAZKFZ010000000
VERSION     DAZKFZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(105..557)
                     /gene="tabA"
                     /locus_tag="ACHOQW_001073"
     CDS             complement(105..557)
                     /gene="tabA"
                     /locus_tag="ACHOQW_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP9572181.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(702..1295)
                     /gene="bdcR"
                     /locus_tag="ACHOQW_001074"
     CDS             complement(702..1295)
                     /gene="bdcR"
                     /locus_tag="ACHOQW_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9572182.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            1366..2079
                     /gene="bdcA"
                     /locus_tag="ACHOQW_001075"
     CDS             1366..2079
                     /gene="bdcA"
                     /locus_tag="ACHOQW_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9572183.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2210..2605
                     /gene="yjgH"
                     /locus_tag="ACHOQW_001076"
     CDS             2210..2605
                     /gene="yjgH"
                     /locus_tag="ACHOQW_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9572184.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            2886..3020
                     /gene="pyrL"
                     /locus_tag="ACHOQW_001077"
     CDS             2886..3020
                     /gene="pyrL"
                     /locus_tag="ACHOQW_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP9572185.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3024..3959
                     /gene="pyrB"
                     /locus_tag="ACHOQW_001078"
     CDS             3024..3959
                     /gene="pyrB"
                     /locus_tag="ACHOQW_001078"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP9572186.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3972..4433
                     /gene="pyrI"
                     /locus_tag="ACHOQW_001079"
     CDS             3972..4433
                     /gene="pyrI"
                     /locus_tag="ACHOQW_001079"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP9572187.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4506..4892
                     /gene="ridA"
                     /locus_tag="ACHOQW_001080"
     CDS             4506..4892
                     /gene="ridA"
                     /locus_tag="ACHOQW_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP9572188.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5098..7794)
                     /gene="mgtA"
                     /locus_tag="ACHOQW_001081"
     CDS             complement(5098..7794)
                     /gene="mgtA"
                     /locus_tag="ACHOQW_001081"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9572189.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7935..7988)
                     /gene="mgtL"
                     /locus_tag="ACHOQW_001082"
     CDS             complement(7935..7988)
                     /gene="mgtL"
                     /locus_tag="ACHOQW_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP9572190.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8173..9120
                     /gene="treR"
                     /locus_tag="ACHOQW_001083"
     CDS             8173..9120
                     /gene="treR"
                     /locus_tag="ACHOQW_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP9572191.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            9239..10660
                     /gene="treB"
                     /locus_tag="ACHOQW_001084"
     CDS             9239..10660
                     /gene="treB"
                     /locus_tag="ACHOQW_001084"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP9572192.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10710..12365
                     /gene="treC"
                     /locus_tag="ACHOQW_001085"
     CDS             10710..12365
                     /gene="treC"
                     /locus_tag="ACHOQW_001085"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9572193.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            12759..14897
                     /gene="nrdD"
                     /locus_tag="ACHOQW_001086"
     CDS             12759..14897
                     /gene="nrdD"
                     /locus_tag="ACHOQW_001086"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9572194.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15087..15551
                     /gene="nrdG"
                     /locus_tag="ACHOQW_001087"
     CDS             15087..15551
                     /gene="nrdG"
                     /locus_tag="ACHOQW_001087"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP9572195.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15596..15982)
                     /gene="cybC"
                     /locus_tag="ACHOQW_001088"
     CDS             complement(15596..15982)
                     /gene="cybC"
                     /locus_tag="ACHOQW_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9572196.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(16166..17518)
                     /gene="pmbA"
                     /locus_tag="ACHOQW_001089"
     CDS             complement(16166..17518)
                     /gene="pmbA"
                     /locus_tag="ACHOQW_001089"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP9572197.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17612..18163
                     /gene="yjgA"
                     /locus_tag="ACHOQW_001090"
     CDS             17612..18163
                     /gene="yjgA"
                     /locus_tag="ACHOQW_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP9572198.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18319..19692)
                     /gene="mpl"
                     /locus_tag="ACHOQW_001091"
     CDS             complement(18319..19692)
                     /gene="mpl"
                     /locus_tag="ACHOQW_001091"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP9572199.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19868..20866
                     /gene="fbp"
                     /locus_tag="ACHOQW_001092"
     CDS             19868..20866
                     /gene="fbp"
                     /locus_tag="ACHOQW_001092"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP9572200.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            complement(20899..21894)
                     /gene="yjfF"
                     /locus_tag="ACHOQW_001093"
     CDS             complement(20899..21894)
                     /gene="yjfF"
                     /locus_tag="ACHOQW_001093"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP9572201.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21881..22903)
                     /gene="ytfT"
                     /locus_tag="ACHOQW_001094"
     CDS             complement(21881..22903)
                     /gene="ytfT"
                     /locus_tag="ACHOQW_001094"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP9572202.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22917..24419)
                     /gene="ytfR"
                     /locus_tag="ACHOQW_001095"
     CDS             complement(22917..24419)
                     /gene="ytfR"
                     /locus_tag="ACHOQW_001095"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP9572203.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(24559..25515)
                     /gene="ytfQ"
                     /locus_tag="ACHOQW_001096"
     CDS             complement(24559..25515)
                     /gene="ytfQ"
                     /locus_tag="ACHOQW_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP9572204.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25825..26355
                     /gene="ppa"
                     /locus_tag="ACHOQW_001097"
     CDS             25825..26355
                     /gene="ppa"
                     /locus_tag="ACHOQW_001097"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP9572205.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26735..27076)
                     /gene="ytfP"
                     /locus_tag="ACHOQW_001098"
     CDS             complement(26735..27076)
                     /gene="ytfP"
                     /locus_tag="ACHOQW_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP9572206.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27079..30858)
                     /gene="tamB"
                     /locus_tag="ACHOQW_001099"
     CDS             complement(27079..30858)
                     /gene="tamB"
                     /locus_tag="ACHOQW_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP9572207.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWKIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVSI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLTGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVADPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30855..32588)
                     /gene="tamA"
                     /locus_tag="ACHOQW_001100"
     CDS             complement(30855..32588)
                     /gene="tamA"
                     /locus_tag="ACHOQW_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP9572208.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32794..33432
                     /gene="msrA"
                     /locus_tag="ACHOQW_001101"
     CDS             32794..33432
                     /gene="msrA"
                     /locus_tag="ACHOQW_001101"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9572209.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33755..35098
                     /gene="paeA"
                     /locus_tag="ACHOQW_001102"
     CDS             33755..35098
                     /gene="paeA"
                     /locus_tag="ACHOQW_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9572210.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(35177..35383)
                     /gene="ytfK"
                     /locus_tag="ACHOQW_001103"
     CDS             complement(35177..35383)
                     /gene="ytfK"
                     /locus_tag="ACHOQW_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP9572211.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35708..36262
                     /gene="ytfJ"
                     /locus_tag="ACHOQW_001104"
     CDS             35708..36262
                     /gene="ytfJ"
                     /locus_tag="ACHOQW_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9572212.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(36325..>37230)
                     /gene="ytfI"
                     /locus_tag="ACHOQW_001105"
     CDS             complement(36325..>37230)
                     /gene="ytfI"
                     /locus_tag="ACHOQW_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFP9572213.1"
                     /translation="GAVELFVKSSYTKTHSNNFFNNIHHAFKKKDWISNYDSLLTLRE
                     FFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLRGSGFEYEEIPI
                     TINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQAIGDVSLGYAL
                     LDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLGFALKIYGGDER
                     FRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSDITKEKICEKY
                     KSTIEAFKKEQLALPVLVRRKNSET"
BASE COUNT         9012 a         9984 c         9244 g         8990 t
ORIGIN      
        1 ataagaacgt ctaagcatta caaattttac tcacaaaaaa atatcaaaaa acgctctttt
       61 cacaaactcg ccgtaaacct ggagttattt acggcgaaaa gtcatcacgc catcagcatc
      121 ttcactactg ctttgcgaac ctgggctggt gcacccactg cgcacagcgg tttatgcact
      181 tcccccggat aaaacacaac aaaatcacct tcattaagga taactgtttt ctcatcaacg
      241 ccttccggca aaaatgcgat gtctttatca gctaaccagt cggtatccgg cgtacctgcc
      301 ggttgtgtgc tgaaggtcat gccttcctga ccttttaaca caatctgaat gtcgagatag
      361 cgggcatggt actccgcacg gcgagcttcg tacggctcgg tcatatcttc cgagataaga
      421 taaaacagac gattaccttc gatatcgtgc ttgccctttg gcgtttctgc cgtaacgtgt
      481 gctttgatat gctcaatcgc ctggcgtaac tcctggggta gccacggctg aagattatga
      541 atatttccga tgatcatctt ttccactcct taattaaaac aatgtttcat ttccaccgtt
      601 atacgaaaag atgatcaatt cagccagcct taacgcgtga attattgtct tatcacaact
      661 tatcggcagg cgcgttcccg cctttcggat caaggccagc atcactcctt gagaatagtt
      721 tttatcgctt cccccgccag tgcagcagtt gcacagagtt gttcgaggga gtgcccctct
      781 cgtgccttcg cagaaagccc tgacatcacg gtactcataa aatcagtcac acattgtgcg
      841 ctttgtggat gcagcctggc gatatagtca taaatggccg tttctgcggc gtgataatat
      901 tgaacggcaa tatcacgcgc ttgtggatca tgactatgaa tgccttcaag aaccatacag
      961 ccagcgcagc cgccgttttg gctatatctg cgcgccgctt cttttaatac ctcagccagg
     1021 cactccccta ccggacgatc atcacgaaga atatcgacaa gcggaatagc ttccgtaccg
     1081 acatattcat tgagtacacg gctaaataac ccagccttac tgccaaaagc cgcgtagagg
     1141 ctcggggggt taataccaag ataatcagta acttcagcaa cactgacagc atcgaaacct
     1201 ttttggtgaa aaagcacttt tgccgcagag actgcctgct caggagcgaa acgtctggga
     1261 cgacctggaa cgcgagattg ttttttagtg accattacaa aacttgttga cagaaagtta
     1321 aaacagtttt gtaatgcatg ttacataata aatcaaggag tccttatggg cgcttttaca
     1381 ggtaagacag ttctcatcct cggtggcagt cgtggaatcg gtgccgctat cgtacgtcgt
     1441 ttcgtcaccg atggggccaa tgtacgattc acctatgcgg ggtcgaaaga tgccgctgaa
     1501 cgcctggcac aagagacggg agcgacagca gtatttacag atagtgctga cagagacgct
     1561 gtcattgatg tcgttcgtaa gagcggcgca ttggatatcc tggtggtaaa tgcaggtatt
     1621 ggcgtctttg gcgatgccct ggaattaaat gccgacgata ttgatcgcct tttcaaaatc
     1681 aatattcatg ctccttacca tgcctctgtt gaagccgccc ggcagatgcc cgaaggtggg
     1741 cgcatcttaa tcatcggctc cgtgaatggc gatcgtatgc ctgttgcagg catggctgct
     1801 tatgccgcca gtaaatctgc cctacaaggc atggcgcgcg ggctggcccg tgattttgga
     1861 ccgcgtggga tcaccatcaa cgtcgtccag ccagggccaa ttgataccga cgctaatccc
     1921 gccaacgggc caatgcgcga tatgttgcat agttttatgg ctatcaaaag acatgggcaa
     1981 ccggaagagg tcgctggtat ggtcgcatgg ttagcagggc cagaagccag ttttgttacc
     2041 ggcgcgatgc ataccattga tggcgcgttt ggcgcataac cgactacgct caattaagcc
     2101 cagccatttc ccatgatgtc tgggttttgt ttactcacgt cgtccgctaa aagcggctcc
     2161 tggtaaatat aaatcttctg ttcatgtcgt aaatcttgag gataaccaaa tggtagaaag
     2221 aaccgctgtt ttccctgctg gccgacattc actgtatgca gaacatcgtt attctgcggc
     2281 tattcgttcc ggcgatttgc tgtttgtttc cgggcaagta ggaagtcgag aggacggaac
     2341 accagaaccc gattttcagc aacaagtcag actggcattt gataatttgc atgcgaccct
     2401 ggcagctgcg ggatgcactt ttgacgatat cattgatgtt acgagctttc ataccgatcc
     2461 agaaaaacaa tttgaagaca tcatgacggt gaaaaatgaa atatttagcg cgccacctta
     2521 tccaacctgg acggcggtgg gcgttacatg gctggcaggc tttgattttg aaattaaagt
     2581 gatagcgcgc atccctgagc tgtaagcaat agtgttagcc gttcgttttc acactccgcc
     2641 ctataagtcg gatgaatgga ataaaatgca tatctgattg cgtgaaagtg aaaaagaaaa
     2701 aagcaggaaa tgtctgcaat tattgatacc gaacgacagt tcccctccag aatcacagta
     2761 aataaaaatg catatatttt gacttataat tcaaataaac cgtttgcgct gacaaaatat
     2821 tgcatcaaat gcttgcaccg cttctgacga tgagtataat gccggacaat ttgccgggag
     2881 gatgtatggt tcagtgtgtt cgacattttg tcttaccgcg tctgaaaaaa gacgctggcc
     2941 tgccgttttt cttcccgttg atcacccatt cccagcccct caatcgaggg gctttttttt
     3001 gcccaggcgt caggagataa aagatggcta atccgctata tcagaaacat atcatttcca
     3061 taaacgacct tagtcgcgat gaccttaatc tggtgctggc gacagcggcg aaactgaaag
     3121 caaacccgca accagagctg ttgaagcaca aagtcattgc cagctgtttc ttcgaagcct
     3181 ctacccgtac ccgcctctct ttcgaaactt ccatgcaccg cctgggtgcc agcgtggtgg
     3241 gcttctccga cagcgccaat acatcactgg gtaaaaaggg cgaaacgctg gccgatacca
     3301 tttcggttat cagcacttac gtcgatgcga tagtgatgcg tcatccgcag gaaggtgcgg
     3361 cgcgcctggc caccgagttt tccggcaatg taccggtact gaatgccggt gatggctcca
     3421 accaacatcc gacgcaaacc ttgctggact tattcaccat tcaggaaacc caggggcgtc
     3481 tggacaatct ccacgtcgca atggttggtg acctgaaata tggccgcacc gttcactccc
     3541 tgactcaggc gttagcgaag ttcgacggca accgttttta cttcatcgcg ccggacgcgc
     3601 tggcaatgcc gcaatacatt ctggatatgc ttgatgaaaa agggatcgca tggagtctgc
     3661 acagctctat tgaagaagtg atggcggaag ttgacatcct gtacatgacc cgcgtgcaaa
     3721 aagagcgtct ggacccgtcc gagtacgcca acgtgaaagc gcagtttgtt cttcgcgcca
     3781 gcgatctcca caacgccaaa gccaatatga aagtacttca tccgctgccg cgtgttgatg
     3841 agattgcgac ggatgttgat aaaacgccac acgcctggta cttccagcag gcaggcaacg
     3901 ggattttcgc tcgccaggcg ttactggcac tggttctgaa tcgcgatctg gtactgtaag
     3961 gggaaataga gatgacacac gataataaat tgcaggttga agctattaaa cgcggcacgg
     4021 taattgacca tatccccgcc cagatcggtt ttaagctgtt gagtctgttc aagctgaccg
     4081 aaacggatca gcgcatcacc atcggtctga acctgccttc tggcgagatg ggccgcaaag
     4141 atctgatcaa aatcgaaaat acctttttga gtgaagacca ggtagatcaa ctggcattgt
     4201 atgcgccgca agccacggtt aaccgtatcg acaactatga agtggtgggt aaatcgcgcc
     4261 caagtctgcc ggagcgcatc gacaatgtgc tggtctgccc gaacagcaac tgtatcagcc
     4321 atgccgaacc ggtttcttcc agctttgccg tgcgaaaacg cgccaatgat atcgcgctca
     4381 aatgcaaata ctgtgaaaaa gagttttccc ataatgtggt gctggccaat taattgcggt
     4441 tggtaataaa agtctggctc cctataatga gccagacttt ttaccgctgt aataaaggag
     4501 aaatcatgag caaaactatc gcgacggaaa atgcaccggc agctatcggt ccttacgtac
     4561 agggcgttga tctgggcaat atgatcatca cttccggtca gatcccggta aatccgaaaa
     4621 cgggcgaagt accggcagac gtcgctgcac aggcacgtca gtcgctggat aacgtaaaag
     4681 cgatcgtcga agccgctggt ctgaaagtgg gcgacatcgt taaaactacc gtgtttgtaa
     4741 aagatctgaa cgacttcgca accgtaaacg ccacttacga agccttcttc accgaacaca
     4801 acgccacctt cccggcacgt tcttgcgttg aagttgcccg tctgccgaaa gacgtgaaga
     4861 ttgagatcga agcgatcgct gttcgtcgct aatcttgatg gaaatccggg ctatcatgcc
     4921 cggattaagt ctgatgacaa acgcaaaatc gcctgatgcg ctacgcttat caggcctacg
     4981 tgattcctgc aatttattga atttgttggc cggataaggc atttacgccg catccggcat
     5041 gaacaaagct tactttgtct gcaatctgaa tcggggctat cgtgcccagt ttattcttta
     5101 ttgccagccg taacgacggc tatagaaccc tttcaccaac tgggttaatg tcatataccc
     5161 tgccagaatc gcaaccagcc acgggaaata gcttaacggc agcgcctgta attgcagata
     5221 actggccagc ggtgaaaacg gcaatgcgat cccgacaatc atcacgatca cggtcatgat
     5281 cattaacggc cacgatgcac agctctgaat aaacggcaca cggcgggtgc ggatcatatg
     5341 cacaatcagc gtttgcgaca gtaagcccac cacaaaccat cccgactgga acagcgtttg
     5401 cgtttccggc gtgttggcat ggaataccca ccacatcagg caaaacgtca gaatatcgaa
     5461 gatcgagctg atcggtccga agaagatcat aaagcgcccc agatccgccg gattccaacg
     5521 ctgcggcttt tgaatttgct cgtcgtcgac gttatcaaac gggatcgcca cctgtgacac
     5581 atcgtacagc aggttctgaa taagcaagtg taacggcaac atcggcagga agggcaagaa
     5641 agcactcgcc accagcacgc tgaacacatt accgaagtta gaactcgccg tcattttgat
     5701 gtatttcagc atgttggcga aagtgcgacg tccctcaata accccctctt ccagcaccat
     5761 caggcttttt tccagcagga tgatatcagc cgcttcacgg gcaatatcta ccgcgccgtc
     5821 cacagaaatg ccgatatccg cagcgcgtaa ggcgggcgca tcattaatac catcgcccat
     5881 aaagccaacc acatgccctt cgcgcttcag taaggtcaca atgcgttctt tatgcatcgg
     5941 cgtcaggcgg gcaaacagcg tagtgcgctg tgcaagattt gccagttcgt cgtcagatag
     6001 cgtttcaata tcactaccaa tgaccacctc tcccgcgtcc agccccactt catggcacac
     6061 tttcgctgca actaactcac tgtcgccagt aaggattttt acggtaattc cactcgcttt
     6121 taatgccttc agtgccggag cggttgtctc ttttggcgga tcaaggaaag caatatatcc
     6181 ttcgaggatc aggtcggatt catccgcccg ctggtaatct ccttcacgcg ctggcaggta
     6241 tttcgtcgcc accgcaacca cgcgcagccc ctgacgattc agcgtatcag taacccgctt
     6301 aatcttacgc agcatgatgt catcgagcgg cacaatctcg ccattgtgac gcacctgcga
     6361 acacacattg aggatttcct gcaatgcacc tttgcaaacc agctgatggt gctcggtatt
     6421 ttctgccact accaccgaca tccggcgacg ctcgaaatca aacggaatct catcgatttt
     6481 ttgccaacga ctggccagcg agcgcgctga ctcttcatcc gtaccttcga gcaccgctgt
     6541 atcgagcagg tttttaagtc cggtctgata atgactattc aaccacgcgc tatgcagcac
     6601 gcgttcgctg gttttaccgg agatatcggt atgattctcc agcacaattt tatcctgtgt
     6661 cagggtgccg gttttatcag tgcacagaat atccattgcg ccaaagttct gaatagcatc
     6721 cagatgtttg acgatcactt tctgtttcga cagctttact gcgccgcgcg ccagcgtcga
     6781 ggtaacaatc atcggcaaca tttccggcgt taggcctacc gcgaccgaaa gcgcaaacag
     6841 cgccgcttcc caccagtcgc ctttggtgta gccattgatt aacagcacca ccggtgccat
     6901 caccagcata aagcgaatca gcagcatact gacgcggctg atcccttgct gaaaggcatt
     6961 cggctcgctt tcctgctcac taacacgccc cgccagttga ccaaaccagg tattggcacc
     7021 tgtagcaatc accattgctt gtgccgtgcc gctcaccacg gtggtgccca taaaacacag
     7081 cgtgtcgcac tccagcggat tgctgtgctc cggctggcga gtggtagcgg ctttttccac
     7141 gggcagagac tcaccggtta acgacgcctg agcgacgaac agatcccgcg cctgcaagat
     7201 acgtaaatct gccgggatca tatctcccgc cgccagtttt ataatatcgc cgggcaccag
     7261 ctggtcgatc gggatctcca gccagccatt ttcgcctttg tcgttaatta cgcgcagcac
     7321 cgtcgcggta ttgctgacca tcgctttcag ggcatctgcc gctttagtgg aacgtgcttc
     7381 ctgaataaag ttcagcaacg ttgaaatagc gaccattagc gcgataacgc ccgcggcaaa
     7441 taaatcttcc gtggcgtaag aaatagcgcc gagaatggtg agtaaaatat taaaggggtt
     7501 gcgatagcag acccataaat gtacccacca cggcgacggt tgttgtgcgg gtaatttatt
     7561 ttcaccatgt tgttcgcggg cagattccac ttccgcctga tttaaccctt ccggatgggt
     7621 gtcgaacgtt ttccacaatt cttcttcggg catcaccgcc attttcaggc aatgcgcact
     7681 taaggacggc gggaccaccg tattcactgt ctgctgcgcg ccaggcaatg gatcacgatg
     7741 aaccagacgg gaaggtaaat ggcgaatgag ccgggtaaaa atttctttaa acataaggag
     7801 tccctccgca ctgtctgaaa cagtacagaa atgtcttcag gcgtggcgaa gccttacctg
     7861 aaggtaataa agctgagcag ggacgttaaa acgtcacggc cttacgcatc cggtaacgcc
     7921 gtgaaaagac gggcttaccg gaaaagtttt aatctccgtc gagggagagg cgtgggatca
     7981 ggttccatat aacctccggt aagtgaaaaa agcgccgcaa ataataagga gaatccaata
     8041 atattcgtag catctatggc gatataatac ctgctggcaa ctaaaccaac gataaaccag
     8101 actttaccca ttgctgaatg cacgggtaaa gttaggctca aataattaaa caacacgtta
     8161 caggacaaca ggatgcaaaa tcggctgacc atcaaagaca tcgcgcgctt aagcggcgtg
     8221 gggaaatcta cagtttcccg ggtgctgaat aacgaaagcg gcgtgagcca gcgcacccgc
     8281 gagcgtgttg aagcagtgat gaatcagcat ggattttccc cttcccgttc tgcgcgcgct
     8341 atgcgtgggc aaagcgataa agtggtcgcc atcattgtta cccgtctgga ttcgttgtca
     8401 gaaaatctcg ccgttcaaac catgctgcca gcgttctatg aacaaggtta cgacccaatc
     8461 atgatggaaa gtcagttttc cccgcaatta gttgccgaac atttgggggt gctgaaacgg
     8521 cgtaatatcg acggcgtagt gctgttcggt tttactggca taacagaaga aatgttagcc
     8581 cactggcagt catcgctggt tctgctggcg cgtgacgcaa aaggctttgc ttcggtctgt
     8641 tatgacgacg aaggggcaat caagatcctg atgcaacggc tgtatgacca ggggcatcgt
     8701 aatatcagtt atctcggcgt gccgcacagt gacgtgacaa ccggtaagcg acgtcacgaa
     8761 gcctacctgg cgttctgcaa agcgcataaa ctgcatcccg ttgccgccct gccagggctt
     8821 gctatgaagc aaggctatga gaacgtagca aaagtgatta cgcctgaaac taccgcctta
     8881 ctgtgcgcaa ccgacacgct ggcacttggc gcaagtaaat acctgcaaga gcaacgcatc
     8941 gacaccttgc aactggcgag cgtcggtaat acgccgttaa tgaaattcct tcatccggag
     9001 atcgtcaccg tagatcctgg ttacgccgaa gctggacgcc aggcggcttg ccagttgatc
     9061 gcgcaggtaa ccggacgcag cgaaccgcaa caaatcatca tccccgccaa cctgtcctga
     9121 tcgtttcctg aacgataaat tgtgatcttc gctgcgtttc gggaacgttc ccgtttttaa
     9181 atttttccgc gcaatatatt ctgcagccaa ccaaaaatgt catctgccat ggggctttat
     9241 gatgagcaaa ataaaccaaa cggatatcga tcggttgatt gaactggtcg gcgggcgcgg
     9301 caatattgcg acggtgagcc actgtattac tcgcctgcgc tttgtcctca accaaccggc
     9361 caatgccaga ccgaaagaaa ttgagcaact ccccatggtg aaaggctgtt tcaccaatgc
     9421 cgggcaattt caggtggtga ttggcaccaa cgtgggtgat tactatcaag cactgatagc
     9481 gtcaaccgga caggcgcagg ttgataaaga gcaggtaaaa aaagccgccc ggcagaatat
     9541 gaaatggcat gagcagttga tctctcattt cgcagagatc ttcttcccgt tgctgcccgc
     9601 gttgattagc ggcggtttga tcctcggttt tcgcaatgtg atcggcgatt tgcccatgag
     9661 caacggtcaa acgctggcgc aaatgtaccc ttccctgcaa acgatctacg attttctgtg
     9721 gttgatcggt gaagcgatct tcttctacct gccggtcggg atttgctggt cagcggtgaa
     9781 aaaaatgggc ggcacgccga tccttggtat cgtgcttggc gtgacactgg tttcccccca
     9841 actgatgaac gcttatctgc tcgggcagca gctgccggaa gtgtgggact ttggcatgtt
     9901 cagcatcgcc aaagtgggct atcaggcgca ggtgatcccg gcactgttag ccgggctggc
     9961 gctgggcgtt attgaaactc gccttaaacg catcgtgccg gattacctct atctggtggt
    10021 ggtgcccgtc tgttcgctga tcctcgcggt gttcctcgcc catgcgctga ttggtccgtt
    10081 tggtcgcatg attggcgatg gcgttgcctt tgcggtacgt cacctgatga ccggcagctt
    10141 tgctccgatt ggcgcggcat tgtttggctt cctgtacgcc ccgctggtga tcaccggtgt
    10201 acaccagacc acgcttgcta ttgatttgca gatgattcaa agcatgggtg gtacgccagt
    10261 gtggccgctg attgcgctgt cgaatatcgc tcagggctcc gccgtgatag gcattatcat
    10321 ttccagccgc aagcacaatg aacgcgagat ctccgtgcct gcggcgatct ccgcctggct
    10381 tggggtcact gagcctgcaa tgtacggcat caacctgaaa tatcgcttcc cgatgctgtg
    10441 cgcgatgatt ggttctggtc tggcaggatt gctatgcggc ctgaacggcg ttatggcgaa
    10501 tggcatcggc gtaggcggcc tgccgggaat tctctcgatt caaccgagct actggcaggt
    10561 gtttgcgctg gcaatggcta tcgccatcat catcccgatt gtactcacct cgtttatcta
    10621 tcagcggaaa taccgcctgg gcacgctgga cattgtttaa ttttcttcgg ggcgcaattg
    10681 cgccccctcg catttgcagg aataacgtaa tgactaatct tccccactgg tggcaaaacg
    10741 gcgttatcta ccagatttat ccaaagagtt ttcaggacac cacgggtagc ggcaccggcg
    10801 atttacgtgg cgttatccaa cgcctggact atctgcataa actgggcgtt gatgccatct
    10861 ggctaacccc cttttatgtc tctccgcagg tcgataacgg ttacgatgta gcgaactata
    10921 cggcgattga tcccacctac ggcacgctgg acgattttga cgaactggtg acgcaggcaa
    10981 aatcgcgcgg gattcgtatc attctcgata tggtgtttaa ccatacctct acccaacatg
    11041 cctggtttcg cgaggcgctg aacaaagaaa gcccttaccg ccagttttat atctggcgcg
    11101 atggcgaacc agaaacgcca ccgaacaact ggcgttcaaa atttggcggt agtgcgtggc
    11161 gctggcatgc ggaaagcgaa cagtactatt tgcatctctt tgcaccagaa caggcggatc
    11221 tcaactggga gaatccagcg gtacgcgcag agctgaaaaa agtctgtgag ttctgggccg
    11281 atcgtggggt cgacgggttg cgcctggatg tggtgaatct gatctccaaa gacccgcgtt
    11341 tccctgatga cctggatggc gacgggcgtc gcttctacac cgacgggcca cgagcacacg
    11401 agtttttgca cgagatgaac cgcgatgtgt ttacgccacg cgggttaatg accgtaggtg
    11461 aaatgtcctc caccagcctt gagcattgcc agcgatacgc ggcactgaca ggcagtgaat
    11521 tgtcgatgac ctttaatttt catcacctga aggtcgatta tcccggtggt gaaaaatgga
    11581 cgctggctaa acctgacttt gtggcgttga aaacattgtt ccgccactgg caacaaggaa
    11641 tgcacaacgt agcattgaat gccttgttct ggtgtaacca cgatcagccg cgcattgttt
    11701 ctcgctttgg tgatgaaggt gaataccgcg tgcctgcggc aaaaatgctg gcgatggtgc
    11761 tgcatggcat gcagggaacg ccgtatatct accagggcga agagattggc atgaccaacc
    11821 cgcatttcac gcgcattact gactatcgcg acgtagagag cctcaatatg tttgccgagc
    11881 tgcgcaacga tgggcgtgat gccgacgagt tattggcaat cctcgccagt aaatcccgtg
    11941 acaacagccg cacgcccatg caatggagca acggcgataa tgccgggttt acggctggcg
    12001 aaccgtggat tggcctgggc gataactatc aacaaatcaa cgtagaagcc gcgctggccg
    12061 atgagtcctc ggtgttttac acctaccaaa agttaatcgc actgcgtaag caggaagcca
    12121 tcctgacatg gggcaattac caggatctgc tgccaaacag ccctgtattg tggtgctatc
    12181 gccgtgaatg gaaggggcaa accttgctgg tcattgccaa ccttagccgt gagatccaac
    12241 cctggcagcc agggcaaatg cgcggcaact ggcagcttgt gatgcataac tacgaagaag
    12301 cctcaccaca accctgtgcc atgactttac ggccttttga ggctgtctgg tggttacaga
    12361 agtaaatctt ccctaagccc cggtaatgcc ggtcattccg gggttttgtc tgtcagcatg
    12421 ttgtttttgt tgatttttca accagcaaat tcattaaaaa atttacataa cgctgtagcg
    12481 cccgtcatcc gtacgctctg ctttttactt tgagctacat caaaaaaagt tcaaacatcc
    12541 ttgatgcaaa gcactatata tagactttaa aatgcgtccc aacccaatat gttgtatcaa
    12601 tcgactataa ttgctactac agctccccac gaaaaaggtg cggcgttgtg gataagcgga
    12661 tggcgattgc ggaaagcacc ggaaaacgaa acgaaaaaac cggaaaacgc ctttcccaat
    12721 ttctgtggat aacctgttct taaaaatatg gagcgatcat gacaccgcat gtgatgaaac
    12781 gagacggctg caaagtgccg tttaaatcag agcgcatcaa agaagcgatt ctgcgtgcag
    12841 ctaaagcagc ggaagtcgat gatgccgatt attgcgccac tgttgctgca gttgtcagcg
    12901 agcagatgca gggccgcaac caggtggata tcaatgagat ccagaccgct gttgaaaacc
    12961 agctgatgtc aggtccatac aaacaactgg ctcgtgctta catcgagtac cgtcacgatc
    13021 gcgacattga acgtgaaaaa cgcggtcgcc tgaatcagga gatccgtggt ctggtcgagc
    13081 agaccaacgc ctcgttactc aacgaaaacg ccaacaaaga cagcaaggtg attccaaccc
    13141 agcgcgacct gctggccggg atcgtggcta aacactatgc acgtcagcac ctgctgccgc
    13201 gtgacgtggt gcaggcacat gagcgtggcg atattcacta tcacgatctc gattactcac
    13261 cgtttttccc gatgttcaac tgcatgttga tcgacctgaa aggcatgctg acccagggct
    13321 ttaagatggg gaacgccgag attgaaccgc cgaagtcgat ctctacggca accgcggtaa
    13381 ctgcgcagat tattgctcag gttgccagcc atatttatgg cggcaccacc attaaccgta
    13441 tcgatgaagt gctggcaccg tttgtcaccg ccagctacaa caaacatcgc aagaccgcag
    13501 aagagtggaa catcccggac gccgaaggct acgctaactc tcgtaccatt aaagagtgct
    13561 acgatgcctt ccagtcgctg gagtacgaag taaacaccct gcacaccgcc aacggtcaga
    13621 cgccgtttgt aacctttggt tttggtctgg gcaccagctg ggagtcgcgc ctgattcagg
    13681 aatctatcct gcgtaaccgt atcgcaggcc tcggtaaaaa ccgtaaaact gcggtgttcc
    13741 cgaaactggt gtttgcgatt cgcgatggtc tgaaccataa aaaaggcgat ccgaactacg
    13801 acatcaaaca gctggcgctg gagtgcgcaa gcaagcgcat gtatccggat atcctgaact
    13861 acgatcaggt agtgaaagtc accggttcgt ttaaaacccc gatgggctgc cgcagcttcc
    13921 tcggcgtgtg ggaaaatgaa aacggcgagc agatccacga tggtcgtaac aacctcggcg
    13981 tgatcagcct gaacctgccg cgtattgctc tggaagcaaa aggcgatgaa gccaccttct
    14041 ggaagctgct ggatgaacgt ctggtgctgg cacgtaaggc tctgatgacc cgtatcgctc
    14101 gtctcgaagg cgtgaaagcg cgcgtggccc cgatcctcta tatggaaggt gcttgtggcg
    14161 tgcgtctcaa tgctgacgat gatatttctg aaatcttcaa aaacggtcgt gcgtctattt
    14221 cgctgggtta catcggcatc cacgaaacca ttaacgcgct gttcggcggc gaacatgttt
    14281 acgacaacga gcagctccgc gcgaaaggta tcgccattgt tgaacgtctg cgtcaggcag
    14341 tggatcagtg gaaagaagaa acgggttatg gtttcagtct ctacagcacg ccgagtgaaa
    14401 acctgtgcga ccgcttctgc cgtctcgata ctgctgagtt tggcgtggtg ccgggcgtga
    14461 ccgataaagg ttactacacc aacagtttcc acctcgatgt ggagaagaag gtgaacccgt
    14521 acgacaagat cgactttgaa gcgccttacc cgccgctggc gaacggtggt ttcatttgct
    14581 acggcgagta tccaaacatt cagcacaacc tgaaggcgct ggaagatgtc tgggattaca
    14641 gctatcagca tgtaccgtat tacggcacca atacaccgat tgatgagtgc tacgagtgtg
    14701 gctttaccgg tgagttcgag tgcaccagca aaggcttcac ctgcccgaaa tgtggtaacc
    14761 atgacgcctc ccgtgtgtcg gtaactcgcc gcgtgtgcgg atatttaggt agcccggatg
    14821 cacgtccgtt taacgccggc aagcaggaag aagttaagcg ccgcgttaaa catttgggaa
    14881 atgggcagat aggttgatct gcactaagca aatgccggat gtgatgtgaa agcgtcgcat
    14941 cccgcgttga ttgccggatg cggcgtaaac gccttatccg gcctacattt ggcaacggct
    15001 gtaggcctga taagacgcga cagcgtcgca tcaggcgttg attgcctgat acagcctaca
    15061 cgcggactga taagccaggc gaaataatga attatcatca gtactatcct gtcgacatcg
    15121 tcaacggtcc cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat gaatgccccg
    15181 gttgctataa caaaagcaca tggcgggtaa attccggtca gccatttact aaagcaatgg
    15241 aagaccagat cattaacgat ctgaatgaca ctcgtatcaa acgccagggt atttccctct
    15301 ccggcggcga tccgctgcat ccgcaaaacg tgccggatat tctgaaactg gtacaacgca
    15361 tccgcgccga gtgtccgggt aaagacatct gggtgtggac aggctataaa ctcgacgaac
    15421 tcaacgctgc gcaaatgcag gttgttgatt tgattaacgt gctggtcgac ggcaaatttg
    15481 tgcaggattt aaaagaccct tccctgatct ggcgcggcag cagcaatcag gtggtgcatc
    15541 atttgcgatg ataagttccc tgttacctga gtgcaggcaa cagggaaatg aggaattaac
    15601 gatacttctt gtgatatgaa ttgcgggtcg ttttcagttg ctctgcagca gcctgcgctt
    15661 cttttacttt accttcattt gccagcttca gcgcgtcgtc aatctgaccg accagaatgt
    15721 cgaaaccgtg gcggaaatct ttcatttccg ggctgtccgg tgatttatct tcgagcttcg
    15781 gcggcgttgc tttttgcgca tccagcgctg cggcgcgcat cttcgttaac gcgtctttga
    15841 cttgcgccgc gttatcggct ttttcgacca cttttaaatt gtcgttgagg gtttccatat
    15901 tgtcttcaag atcagcagca aacgacgccg aactgaatac caacgaggag actgcaagaa
    15961 tagctaacag gctttttcgc attgctcact tcctttttta tttacacttc atccttcaca
    16021 ctgcctcatc gacgactacg ctcattcgac cgggtcacgt actgcgagta cgctccccgg
    16081 ccctcattca ctttccgtct cgatgcagtg cgaatgattt tgtgtaattt tttagtttat
    16141 agcgcggcag gtcgcgccag tttttttact gtccggcgat tttcatctcc ggcaacagca
    16201 cagaaccaca ctgtatatta ctgcgtgttt caatatcgtt accgacggtg acaatattgc
    16261 gccacatatc ttttaaatta cctgcgatgg tgatttcgct aaccggatac tgaatttcgc
    16321 cgttttctac ccagaagccc gccgcaccac gggaataatc accggtaatt gcactcacgc
    16381 cctggcccat caattccgtc accaccagcc cggtgcccat ctctttgagc atctgctcga
    16441 agcttagacc ttgtccggca atccgccagt tgtgaatacc gcccgcatgt ccggtgcttt
    16501 tcagccccag tttccgcgcc gagtagctgg tcagcagcca ctgagtcagg atgccatctt
    16561 taataatatc gcgacgctcg gtgcgcacac cttcgctgtc aaatggcgtc gatgccagcc
    16621 ctttcagcag atgcggatgc tcttcaatgg tcagccagtc cggcagaatt tgtttaccca
    16681 gcgaatccag caggaaggta gatttacggt aaaccgatcc acccgctatc gcccccacca
    16741 gatgcccaaa aagcccggtt gccacttcat tggcaaaaat gaccggcgct ttcatggtgg
    16801 agagtttacg cggtgacaga cgcgataaag tacggcgagc gcaatcggcc ccgacccact
    16861 ctggcgtctg caaatcgctc atcgcgcggc caatggtgta ggcgtaatca cgctccatat
    16921 cgccattttc ttcggcaatt acacagctgg agagcgaatg acgcgttgag cagtaaccct
    16981 gcaacatgcc gtggctgttg ccaaaaactt tgacaccgta gtggctgtta aagctgccac
    17041 cttcggtatt ggtgatgcgt ttgtccgcct gcaatgccgc ctgttctgcg cgggccgcca
    17101 gttcaatggc ttcatccggg gaaacttccg cagggtggaa caagtcgaga tccggtgcgt
    17161 caaaggccag cagctctttg tctgccacgc cggcacaggg atctggcgag gtataacggg
    17221 caatatccag cgccgcctgt acagtacggg caatggcctg cgggcttaaa tcggtggatg
    17281 atgcgctacc tttgcggttc tggtgataaa cagtgatccc cagcgcgcca tcgctattga
    17341 attcaacatt ctccacttca ccataacgcg tgcttacgct aatgccggtg gtcttgctga
    17401 cggcaacttc cgcaccgtcc gatttgcctg aggccaactc cagcgcagtg gaaactgctt
    17461 cttccagaat cttgcgctgc gcttcaactt gagagattac tttcattgca agtgccataa
    17521 tgtagagagt ttctctgaag tctaacagag aaccgttttt cagtgcgcat cttaactggt
    17581 aacattagcc tcttttttta aggagcctga gatgactaag cagcccgaag actggctcga
    17641 cgacgttccc ggtgatgaca tcgaagacga agacgatgaa attatctggg tcagtaaaag
    17701 tgaaattaaa cgtgatgccg aggagctaaa acgccttggc gcggaaattg ttgatctggg
    17761 gaaaaacgcg ctggataaga tcccgttaga tgcggatctg cgcgcggcta ttgagctcgc
    17821 ccagcgtatt aagatggaag gtcgccgccg ccagttgcag ctcatcggta agatgctgcg
    17881 ccagcgcgat gtagaaccga tccgtcaggc gctggataag ctgaaaaacc gtcacaacca
    17941 gcaggtggtg ctgttccata aactggaaaa cctgcgcgat cgtctgatcg atcaaggtga
    18001 tgacgccatc gctgaggtat taaatctgtg gcccgatgcc gatcgtcagc aactgcgtac
    18061 tctgatccgc aacgcgaaga aagagaaaga agggaataag ccgccgaaat ccgcacgcca
    18121 gattttccag tatctgcgcg aactggcgga gaatgaaggg taattttcgt tgttgatgtg
    18181 agtatggttt gccggatgcg gtgcacctgt aggcctgata agacgcatca agcgtcgcat
    18241 caggcactat gctcaaatgc cggatgcggc gtgaacgcct tatccggcct acgcacacta
    18301 tctcaggctg aggccgaatt actgcgcagc ttccgccttc ttcgccagac catccagtag
    18361 tttctgatgg atcccaccaa aaccgccgtt gctcatcacc agaatatggt cgccaggctg
    18421 agcggttttc accaccatat ctgccagcgt atccacatcg ccactccagt gtgcaggctg
    18481 aacgcaggct tctgccactt ctgccacctg ccacggaata tgcgccggtt gcagcaggaa
    18541 gacttcatcg gcacgaccta atgaaggtgc cagatcgtct ttgcagatcc ccattttcat
    18601 ggtattcgag cgcggctcca gcacagcaat aatgcgcgcc gtaccaccaa ctttgccacg
    18661 cagcgccgcc agcgttgcca gaatcgccgt cggatgatgg gcaaaatcgt catataccgt
    18721 gacgccattc gcttcaccac gcaactccag acggcggcga gcgttaataa acgaacccag
    18781 cgcgttagcg gcatctgccg gcgctacacc aacatggcga gccgctgcaa tcgccatcag
    18841 gccattgtgc atattatgtt cgcctaccag cgaccatttc acttcgccca ctttttcgcc
    18901 atccagcaaa acttcccatt cggaagcatc ggtggtcagc tttttcgcct gccagtgacc
    18961 ctgctcaccc accagctcct gctcgctcca gcagcccatc gccatggtct gtttcaggtt
    19021 gatgtcattt tccggccaga taatacgacc ctgccccgga acgatacgca ccagatggtg
    19081 gaactgtttc tggatcgctt tcaggtcgtc aaagatatcg gcgtgatcga actcaaggtt
    19141 gttgaggatc agcgtacgcg ggcagtaatg gacaaattta gagcgtttgt cgaagaaggc
    19201 gcagtcgtac tcatccgctt cgataacaaa gaagtcgctt tcgcccagac gcgccgaaac
    19261 ctcaaagttc cccggcacac cgccgattac aaagcccggt ttgtaaccac actgttccag
    19321 aatccaagtc gccattcccg cggtggtggt tttgccgtgt gtaccggcaa cggccagcac
    19381 ccagcggtcg cgcagcacaa aatcgtgcag ccactgtgga cctgacatat aagggatgtt
    19441 tttttccagt accgcttcca cacacggatt tccacgggtc atggcgttgc caataatcac
    19501 cagatccggc tgcgggtcga gctggctggc atcgtaaccc tgaatcagct caatgccttg
    19561 cttctcaagt aaggtgctca tcggcggata cacattggcg tccgaacccg ttacttcatg
    19621 gcctaactgg cgcgccagca tcgccagacc gcccataaac gtgccacaaa ttcctaaaat
    19681 atgaatgcgc atgcgtcact atcctttttt aatctgccgc tcattttacg catatgtcgg
    19741 gcaagtgaga aacgcatttc aggataatcc gtaatttgct ggcgcgattc acctgagcgc
    19801 aacattgaga ttatttgtta agattgttgc ggtcgcttta ctccataaac attgcaggga
    19861 aagttttatg aaaacgttag gtgaatttat tgtcgaaaag cagcacgagt tttctcatgc
    19921 taccggtgag ctcactgctt tgctgtcggc aataaaactg ggcgccaaga ttatccatcg
    19981 cgatatcaac aaagcaggac tggttgatat cctgggtgcc agcggtgctg agaacgtgca
    20041 gggcgaggtt cagcaaaaac tcgacttgtt cgctaatgaa aaactgaaag ccgcactgaa
    20101 agcacgcgat atcgttgcag gcattgcctc tgaagaagaa gatgagattg ttgtctttga
    20161 aggctgtgaa catgcaaaat acgtggtgct gatggaccca ctggatggct cgtccaacat
    20221 cgatgttaac gtctctgtcg gtaccatttt ctccatctac cgccgagtta cgcccgtcgg
    20281 cacgccggta acggaagaag atttcctcca gcctggtaac aaacaggttg cggcaggtta
    20341 cgtggtatac ggctcctcta ccatgctggt ttacagtacc ggatgcggtg ttcacgcttt
    20401 tacttacgat ccttcgctcg gcgttttctg cctgtgccag gaacggatgc gcttcccgga
    20461 gaaaggcaaa acctactcca tcaacgaagg aaactacatt aagtttccga acggggtgaa
    20521 gaagtacatt aaattctgcc aggaagaaga taaatccacc aaccgcccgt atacctcacg
    20581 ttatatcggc tcactggttg cggatttcca ccgtaaccta ctgaaaggcg gtatttatct
    20641 ctacccaagc accgccagcc acccggacgg caaactgcgt ttgctgtatg agtgcaaccc
    20701 gatggcattc ctggcggaac aagcgggcgg taaagcgagc gatggcaaag agcgtattct
    20761 ggatattatc ccggaaaccc tgcaccagcg ccgttcattc tttgtcggca acgaccatat
    20821 ggttgaagat gtcgaacgct ttatctgtga gttcccggac gcgtaatctc ctgcgcctct
    20881 ccacacggag aggcgatttt attgctgcgc aatgttgact cttgtcacag gcgaactctg
    20941 acgattctcc cacagcaccg ttaatccacg ctgtaatgct ataaaaataa acaacaaaat
    21001 accgatggcg attttcgtcc accatgagct cagcgtacca tcaaagttta tgtaagtctg
    21061 aatcagtccc tgaatcgcca cgccaaaaag cgtccctaat accgttccaa cgccaccgct
    21121 caaaagcgta ccgccaatca ccactgaggc gatagcgtcc agttccacac ctacgcccgc
    21181 cagcgcgtat ccggcctggg tataaatcga gaagacaatc cccgccagtg tcgccagtcc
    21241 ggtagagagc atataaatgc gaatagttgt gctgcgagtg gaaatcccca tcaggtttgc
    21301 cgacgtcgca ttgccgccaa tggcgtacac ctgattacca aagcgggtac gatgcgcgag
    21361 gaatatgccg ataaccacca ccgccagcat cagcagcccc atcgcactta agcgaccgcc
    21421 gccaggaatt ttccatgcaa ggcttgagag cgtgtcataa acaggatggt ttatcgggat
    21481 cgactcttcc gaaacgaggt agctgacgcc gcgcaaaaag aacattcctg ccaaagtaat
    21541 gataaatgcc gggatcttca gggcgtcgat caaaagcccc ataaatgcgc caaaggcaca
    21601 gcccattacc agcaccagcg gaaatgccag caacggcgag aggccgaaat cgccaatcac
    21661 ttttgccaga aacacgccgg taaaggcgat caccgaacca acggagagat cgatcccacc
    21721 ggagaggatc acaaaggtca tgccaacggc gattatccca agaaaggcat tatcggtcag
    21781 gatattgcag atcactcttg tggaagcaaa accgggaaac tgggtcaggc agtagagata
    21841 acccagcaca aagacgccga tggtgatcat cagtggcaaa ttacgtttta tcacggctac
    21901 gcactccttt aatcagactg ataaagcgtt gcgactggac aatcagcacg caaagcacca
    21961 ccaccgcttt caccacctgg ttcatctctg gcggaaagcc cgaaagcaaa attccggtgt
    22021 tcatcccctg aataatcagc gcccccacca ccgaaagcag taggttaaaa cgtccgccca
    22081 tcagcgaacc accgccaatc accaccgcga gaatggcgtc cagctccagc cataatccgg
    22141 cgttgttggc atcggcaccg cgaatatccg ccgccacgat aatgcccgca atcgccgcac
    22201 acagcccgct caacacgtag gtaagcatga cgatgatccg cgtgtttacc ccggcatttt
    22261 ttgccgcccg aatgttgata ccaacggctt cgataaacat ccccagcgcc gttttgcggg
    22321 tcaacagcca gaacaggata agcgtcagca ccgcgataat gaccggcgtt ggcaggaaca
    22381 acagcgatcc actgccgaac catgagagat ccggcgagtt aaacgtgatg atctgtccgg
    22441 aggtgatcag ttgcgccacg ccgcgcccgg cgaccatcag gatcagagtg gcgacaaacg
    22501 gctgaatttt gaggatcgct accagtatgc cgttccacaa tcccgccagg atgccggtgc
    22561 ccagggcgct taacaaaaca atcggcaggc tgaatcccgc gaccgtcatc gcagccgttg
    22621 tggctccggc gatagccatc accgccccta cggagagatc aatcccacct gttgcgatca
    22681 ccagcgtcat accaatcgcc agtagcgcaa cgggggcggc gcgattaaga atgtcgatag
    22741 ggctaccgaa caaacgccca tcctgaagca ccacctgcca gaaatgcggg gccaccaggc
    22801 tatcgaccag cagcaccagc aacagtgccg ccagctgcgg cattcccgtt ggccagcgaa
    22861 agcgcctttt tggcggcgta gtgtccggga gagattgagg catcacactg ttctccttac
    22921 gccgcaatgg cattcatgat cgccggaacg gaaagctctg ccagcgggat ctccgccacc
    22981 tgtttgcgat cgcgcatgat gatcacccgg tcggcatagc ccaccagttc ttccagttcg
    23041 gaggagatca ccagcagcgc cagaccatcg gcgcatagcg tttcaatcag gcggatgatc
    23101 tcggcgtggg caccaacatc aatgccgcgg gttggctcat cgaggatcag aaattgcggt
    23161 cgggtcagta gccaacgtga aagcaacact ttttgctgat tgccgccgga gagaaattca
    23221 atcggttgtt cagttgaagg tgtgcgaatg ccaagctggc ggataaagcg ttcggcaatc
    23281 tcttgctgtt ctttgcggga aatgggacgt agccagccgc gctgggcctg gagagcgagg
    23341 atgatatttt cccgcaccga ggcggcagcg atgatgccat cggttttcct gtcttccggg
    23401 cagaagccaa tgccaagtac cgaagcctga tgtggcgatc gcaggttttg cggtttgcct
    23461 ttgatcaacg ccgtgccgct gtcagcaggt ttgataccga agatcacttc ggcggtttcg
    23521 gtacgtcctg atcccagcaa tccagccaga ccgacgatct cgccggggcg tacttcgaga
    23581 tcaaacggtg cgatcgttcc ttttttgccg taatttttga acgcggcaac gggtttgtcg
    23641 ctcaacaatg ttcgcccggc acgctgtagc gcgtgggtat ccagctcgcg ccccagcatc
    23701 atttttacca gttcgatctg cggtagctcg cacgtttccc gacagcctac gaaactgccg
    23761 ttgcgtaaga cggtgatccg atcgctgacc tgatagacct gatcgagaaa gtgagtgaca
    23821 aagatcaggc tgacgccgcg atcgcgcaac tgacgcatca ggtcaaacag taactccact
    23881 tcctgggtgt cgagactggc ggtgggttca tcgaggatca gcactttggc agagagatcg
    23941 atagcccggc aaatagcgac gatttgctgc atcgcgactg aaaagcggtt gagcggttcg
    24001 cgcacgtcga gggagaaacc gtaagatgcc atcagttcgg tggcgcgctt ttccatctct
    24061 ttgcggcgta gaaggccgaa gcgtttgggt tcgcggccta taaatagatt atcagcgacc
    24121 gacatattgg gtagcaggtt gacttcctga tagacggtgc caatgccgag ttgttgcgcg
    24181 tgggcggtat ttttcggtga gatagcctgg ccttccagcc agatggtgcc gcgatcagcg
    24241 tggtatacac cagttaatgc tttgattagc gttgattttc ccgccccgtt ttcaccgagc
    24301 agcgccatga tttcgccacg gcgcaggctg aaatcaacgt tgtctaacgc tttgacgccg
    24361 gggaaaaatt tacttaatcc ttcggtgcgg aggatctcct ggtgttggtc ggtcgtcatg
    24421 gtttcccccg gtgtaaagcg tgctcccagt aggcatgata agacgcggta agcgtcgcat
    24481 caggcttcag tgcaccatcg ccggatgcgg cgcgagcgcc ttatccggcc tacgaatcgg
    24541 catcaggcat agcaaccctc aataccccat atttttcttc ttctctaact cttcttttgc
    24601 ggtatcaggc aggtaaaggg tggatttggt taacgtcagc ttttcaggca tggtgccgtc
    24661 ttttttgtat ttctccagcg cgtcgaaggc gggacctgcc atattcggcg tcagttcaac
    24721 actggcgttc gcttcgccat caatcatcgc tttgtagata tccggtacgc cgtcgataga
    24781 acctgtcaga atatctttgc ctggtttcag gcccgcttct ttaattgcct gaattgcacc
    24841 aatcaccatg tcgtcgttat gggcgtaaac catgcagatg tttttgccgt tgttttccgc
    24901 tttgataaag ctctccatga cttctttgcc tttactgcgg gtgaagtcac ctgactgcga
    24961 gcggataatt ttgatatttg gcgcattctt aatggcttcg gcaaagcctt tcttacggtc
    25021 aatggcgacg ctggccccaa cagtgccctg cagctccacc acgttgcatg gtttgccatt
    25081 cacttctttt accagccagt caccaatcaa cttgccttcg agaatgttgt cggcagtgac
    25141 ggtggtcata tagagagatt tgtctttcac atcaatagaa cgatcgagca agaagaccgg
    25201 gatttcggca tctttcgcct cttttaatac cggttcccaa ccagtcgcaa ccaccggagc
    25261 gataaagatc gcatccaccc cttgcgcgac gaaggaacgt accgctttaa tctggttttc
    25321 ctgcttttgc tgaccatcgg caattttcag cgtaattccg cgtttttcgg cctcactttt
    25381 cgccacattg gtttctgcgg cgcgccagcc agattccgat ccgacctgcg aaaatcctac
    25441 ggttaatgga gcggccaacg ccatagacga catggctgcc gagactgcag agactacaag
    25501 taagcgtttc cacatagggt tgtcctcgtc ggggtaatta ttattggtga aaagatgttc
    25561 gcgaaaaaac tatagacaat tcgttatgta acggattgcg ttacatcaca cttcaaagaa
    25621 gtaaataaaa cgttaatcac aagtttgtaa tcgctttcat ctcactatga aaaatgcggc
    25681 tacggttatg gattttcctg ctctgtatac cgtcttaaaa ttggcgaaaa aggaaaatga
    25741 agacgaaaac aagcgaagac attcggcgcg agttggctat aatactcggc acttgtttgc
    25801 cacatatttt taaaggaaac agacatgagc ttactcaacg tccctgcggg taaagatctg
    25861 ccggaagaca tctacgttgt tattgagatc ccggctaacg cagatccgat caaatacgaa
    25921 atcgacaaag agagcggcgc actgttcgtt gaccgcttca tgtccaccgc gatgttctat
    25981 ccgtgcaact acggttacat caaccacacc ctgtctctgg acggtgaccc ggttgacgta
    26041 ctggtcccga ctccgtaccc gctgcagccg ggttctgtga tccgttgccg tccggttggc
    26101 gttctgaaaa tgaccgacga agccggtgaa gatgcgaaac tgattgctgt tccgcacaca
    26161 aagctgagca aagaatacga tcacattaaa gacgttaacg atctgccaga gctgctgaaa
    26221 gcgcagatcg ctcacttctt cgagcactac aaagacctcg aaaaaggcaa gtgggtgaaa
    26281 gttgaaggtt gggaaaacgc agaagccgct aaagctgaaa tcgttgcttc cttcgagcgc
    26341 gcaaagaata aataagttct tctagcgcaa taaccctgaa cgccgggctt cggttagtaa
    26401 gggttttttt atgcccgcga taaataaact ctctattcca ccatcattat tctcagcggt
    26461 tgcaaggctt gaacggtaag aacaagcaaa cccgaccacc attttgctgt tcatagccac
    26521 ttgctggaag ttagccgacc tcactcatac tcaccgttca aagcgtgacc ccgttttacc
    26581 cctgcacatt tgtgaacgcc agcagagttt tttatgcgat tgcagatatg gaaagcggct
    26641 cggagagaaa agcaaaagat ggaaaagtta caaaaaaaaa tcgcgagata catcgcataa
    26701 aacaacgcca cccgaaggtg gcgtgctttt attattactt atccctgtct aaccagtcgc
    26761 cgctttcaat tagctttaat ccatcgacgg gtcgttgata aacgtacatc catgcactcc
    26821 cgtacggcgt ctgaatcaac tggcgcgcgt attcaccgcc cctggtgcgc aaggcatcaa
    26881 gttcggccag cgtggcgttg tcaatacgat aaacttcacc gtgtactgtt ccgttccccg
    26941 gaactgcgcc tggatagtgg cccaggctat acaactggta gttatcgata ctgaaatcgc
    27001 ccagtaactg ggcattggtc atccagtgac tgttgccttg tttgtggcgt aaactgccgt
    27061 agacaaatat tcgcattgct aaaactcgaa ctgatagagc aaatccagtg cctggtctac
    27121 accagacacg gcttccagat atagcttagg catcaggcga taacgtaacg tgagtgttgc
    27181 tatagagtca aatataccca cgccgtattt cacttgcaga cctggcaata catagccgct
    27241 gaccactacc tgggaggagt cgcctactcc ctgggtgtcg agcgctaaat tgcttacgcc
    27301 aaacgtctcg ccgattttac ccacaatctg gccactttgc gcaaccccca gaccaatcag
    27361 catcgaggtc attgccgcac tgtcgctctg atcgctctcc agcccttgtc cacgtagcaa
    27421 ataagacaat gcagcttgtt gcgacatcgc cgggtcagag aagatctccg ctttcggttc
    27481 gtccgccaga ccagtgacgc gaactccggc gattacgtcg tcttctgtag catccgggtt
    27541 acgaatagct tcaatattaa gatacggttg atccggcgga ccagagaaca gtaactcacc
    27601 tttacgcaca atcagatcct gaccataggc atggaagcgc ccttcaggga tgttgatctg
    27661 cccgttcagg cccagccctt gtttgtcctg aacgacgtta agatcgcccg tcagccgcgc
    27721 tttcaggcca aaggcgtcaa tgcgcacatt gttgccaacg tggacaatca ggttgctgtt
    27781 aatcggaatc gacgccgttt ttgcctcttc cggttgcagg ttatcgttaa gcatcaccac
    27841 atcgctggag acgcccaccg cgctttccgg cagatcgtgc accacgatgc gcgcccacgg
    27901 gacatccacg cgaccatcaa gggtaaacag gtttggtgta gcctcaaata caacatctgg
    27961 tgatacatcc attcgtacca tcggcggcac ggtgatacgc actttactgc ccttcgctgt
    28021 tacccgcgcc cgccagtttt caatctggct ccagtcggcg tcgccgttca ggtagatctc
    28081 accctgctgg gtccgtactg taccggcaag cgtcgagcgc ataccgttaa agttgaccgc
    28141 aagctggctc ggctgcatat caaacggcat aaagttgccg tcgatatcca cacccgtaac
    28201 ctgtagctgc ccaaacaact gcgggctttg cacatcacca cccagacgta agttggcact
    28261 caccatcccc gcagcttttt ccccacgggt aaagatgggg tttatcatcg caaggttgaa
    28321 gttacggata ttgacgttgc caccaagatt acggcggcct tgcggatcgg ccacctgcac
    28381 ctgtccatca aactggccgt tattggtcag gcggatggtc cagcccaatt cggcacggtt
    28441 gttacgcaac tccgccgtca gattcagcgt ctgaaacgcc acgggcagcg ccgcatcgtt
    28501 gacggtttgc gttacctgca cgttacgccc cgaaagggtg atactgccct gcggcagccc
    28561 ctctttcgtg gtgtcccagg cgacatccgc tttacccgtg aagataccgc tggcctgagt
    28621 ggtttctggc ataaacggtt tcagcatcgc caggtcgaag cggttgagat tcaccaccgc
    28681 acgcccttcg gcccccgcat cgatagtttg cggcacgcac agttccgcat tcgggttaag
    28741 ccagcagtgt ggcccgatgc tgattttttg ctccttgttg cgataatcca gcgcaatatc
    28801 gcgggtcagc gaccacgggc cgactggcgt ctggaagcgg gtattgctaa gcgttccctt
    28861 ccagcgttct tctttgcgat caaaacttcc tgtcagatta agctgtccgg aaaccggttc
    28921 gccctgaatc cgcaactgta gctcgtgctg tttttcgcta cctttggcat tcagggtgac
    28981 gaggttgata ctcacatcgg gctgcgagat ttgctcaacg cgtacgtcaa gtttacccgc
    29041 aatctgatcg gtggatttga tatcgccttc cacgcgaacc tgcgccacgg aaagttcctg
    29101 ccagcgcagg ccgcgcgcgg tgatatctgc cagtagttgt ggcgcttcca ccgtgccgcg
    29161 tactttcacc agccctttcg ctgtaccgcc aagccccggc agcgcgttat ccagccccgg
    29221 cgcgttgatg gtggcatcaa gattgagatc ttttaccccc agctcgcctt tcacttcggc
    29281 actgtttggc cccagttcca gatgcagccc tgggatcttc cactgcatat aactgttgcc
    29341 tttcagcgtg ccgtcaacgt tcactttgtt ctgtttgacg ttaccggtca gcttcagttc
    29401 tggcacctcc atctgccagg tgccgccgta caggctaccg cgggttttaa tcaagccatt
    29461 gagtttcgac ggccactccg ggaactcttt ggcggtgtta atgccgttaa gcgttagctc
    29521 accgcgccaa ctaatggcct gctgccagtc gagcaacgct ttgagttcag ttttcccttc
    29581 cagcgccgcg acggtgagtt tgtcgagatt gacctgctgt tcattacctt tggcatcaag
    29641 ggtaatggtg gctggcggga tctcctgtcc cttcactgcc gtgcgcatag agagcgtgta
    29701 atcggtcatt ttgccggtaa gtttcagttt cagatcatcc gcctgatact gcttctctcc
    29761 agtgaacggc cagtaaagct gtttgctgtt cacttccacg ttgagcggca atccggcttc
    29821 cgccagtcgc gtctgggcgc gtaaatccat atccaccgga ccagaaaggt taacgcctat
    29881 ctccagctgt tcgcgcagcg cgccgcccac tttcagcttc accttttcac ctttcaacgg
    29941 ctccacgttc agtgtgctgt tgagggtgat atccaccggc cagttgtctg acagctgcgc
    30001 cgtgccgctg gcgttgacaa tcccctggtt ggagtcgata tccagcgcgt ccagtttggt
    30061 attgccgtcg atgctgctca ctttcagcag catggtgcgc acggtgatgt ctgtgtcgcc
    30121 cgtcacgcgc agttgctcgc ccttaaactc ttcaatgttc aggttgagcg gcagatgcac
    30181 gtcggtcatt tccggcaata ccgggcgaga aaagagatct ttcagcgttt cgccgagcgg
    30241 cttttcatcc ggctgcggat tttcaatttt cggttcgaca acttcttcct gcgccacttc
    30301 cgccactttc ggcaaagcaa tcaacaggcc tttcagcgac gtcggtttca gggtcagggt
    30361 tttctcctgc cagttcaggc cggaggtgaa gtccatcacc gataccgtgg tgtcatcaat
    30421 cttgatgttg acgttgtcca gcgccacccg tgtcagggtg atgggatacg gcgtggagag
    30481 atccagcggg ccgctatctt cctcttcttc aacctgttca gaaggaggca tttttttact
    30541 gtcgatgttg acctgaatgt ctttcagcgc caggtcatta atacaaacgc tactgttcca
    30601 caggcactca agcccgaccg ccagatgcag attgcccgct tttaccgcca cgcctggctg
    30661 ctcataacga acgtcagaca aggtgagatc gcgccagccg ccggtgactt tgccaatatc
    30721 cagtcctggc acccagcgat ccgccgcttt aaataccaga tgcaggccgc tggtggtgcc
    30781 caccagaaac gccaccgatc ccagcaacag caagataacg ataaccacgc cgaggctgat
    30841 ttttttccat aaactcataa ttctggcccc agaccgatgt aaaactgtaa cccgtgttcg
    30901 tctttatccg cgaccggtac ggcaaaatcg agtttgattg gcccgaccgg cgattcccag
    30961 cgcacgccga ccccggtacc ggttttaaag tcgctgcggc gaatatcgct taccgcttcg
    31021 ccgctatcga caaacaccgc gccccaccat tttccggtca cgttgtactg gtactccagc
    31081 gatccggtta tcaacttcga ggcccctttc aggtcaccgt tggcgtattt cggagcgatt
    31141 gatttgtatt tgtagccgcg aatactgcga tcgcccccgg cgaagaaacg cagatccggc
    31201 ggtactttgt cgaaatcacc ggtttcaatc cagcccagcg tgccgcgtgt cacaaaacga
    31261 tggcgatcgt acagtgtgcg gatccagacg ttctgcgcct ggaaaacgga gaaatcgaca
    31321 tctgaacccc aggccgtgtt ggagtagtcg atagagtagc gttgcgagtc gccccaggtt
    31381 ggcatcaggc caccacgaga acgcgtgcgg ctaatcatca ccccaggata aaacagcatc
    31441 gtggtgttgg taatttcacc ctgagtaaag tggtcgagac tccagcgcag gttaatggca
    31501 cgctgccagc cgctggagag atcccagtag cgagaagcca ccagcgtagt ggagtcagac
    31561 tcggtatcgt tcaggtcagt gcgcttaaaa ccgccctgca ccaaataata ttgttccagt
    31621 ggattcttca gcagcggcat tttatagctg aagtcgaggg tctgttccgg cgcggaaata
    31681 ctggtactgg tggtcagact gtgaccgtat gagttcatcc acggtttttt ccacgtcgct
    31741 ttcacgcgcg gtcccacgtc cgtagagtaa ccgaccccgg tttcgatagt gttttctgtt
    31801 cgcggcgaaa ccacgcccgt caatggtaat actttcgttt cgcgcgcttt atcaaattgt
    31861 ggagccacca ccaccgagtt aaaccagccg gtagcagaaa gtcggcggtt cagttctgcc
    31921 agatctttcg attcgtactc atcgccctct ttaaacggca ccagattttg caggtattca
    31981 tcgcggattt gtgatccttc aaaggtcaca tgcccaaagc ggtaacgttc gccactgtta
    32041 taatcaatat cccagaaggc tttatgcagg ccgagcgcaa tgcccagctg cgctttggta
    32101 aattcgctat cgaaataacc tttacgcaac gcaatgctgg ttaaggactt tttgaaattt
    32161 tcataatcgc cctggttcag taccgtgcca atagccgggc gagtatcgag caatttcaaa
    32221 tagtctttat cggtccgcgc gcccccgcgc aataccacat cggtgccgcc aattaacacc
    32281 ggcacgcctg gcgtgacttt ggcgatcaat acctgccgcc ctttctttgg cggtggacgg
    32341 agatcaaatt caatggtcgg ctggtaataa cccagcgctt tcagaccttc gcggatagca
    32401 tcatcgacgc gtgcgcgaaa gcgacggtct ggcgtcactt catcactttc aatcgtagaa
    32461 agctgcgcac gaacgttctt ttccagctgt cccgataacc cctcgacctg tagacggacg
    32521 ttcgcggcga cggcagatcc gcttaagcag agtaagctta cacagcataa ctgtcggata
    32581 tagcgcacat tttctcctga atatcctttt ttcctgcccc tggaacgccg ttaaacggct
    32641 taacaaaaat ccagtaatat ggattaaaaa gcagactaaa ccccaaatat ttcttatgtt
    32701 ttactttaga cctattcacg gtggttattg tgtgcaaata cgcctcttgt tacaacctta
    32761 accccaatga ccgattttcg ggagagcgac accatgagtt tatttgataa aaagcatctg
    32821 gtttcccccg ccgatgccct gcctggacgt aacaccccga tgcccgtagc cacgctgcat
    32881 gcggttaacg gtcactcaat gaccaatgta cctgacggaa tggagattgc catttttgcg
    32941 atgggttgtt tctggggtgt ggagcgtctg ttctggcagt tacacggcgt ttacagcacc
    33001 gccgcaggtt ataccggcgg ctatacgcca aatccgactt atcgggaagt gtgctccggt
    33061 gatactggtc atgccgaagc ggtacgcatt gtttacgatc cttccgtcat cagctatgag
    33121 cagttgctac aggtattttg ggagaatcac gatcccgccc agggaatgcg tcagggcaat
    33181 gaccacggca cgcagtatcg ttcagcgatt tatccgctga ccccagaaca ggatgccgca
    33241 gctcgcgcca gtctggaacg ttttcaggcg gcgatgcttg ccgccgatga cgatcgtcac
    33301 atcaccacgg aaatcgctaa cgccacaccg ttttattatg ccgaagatga ccaccagcaa
    33361 tatctgcata aaaacccgta cggttactgt ggaattggcg gaattggcgt ctgtctgcca
    33421 ccggaagcat agcgttacgg gtacaaatgt agattgttga taaagtgcgt tttgtttatg
    33481 ccggatgcgg cgtaaacgcc ttgccaggcc tacaaaatcg tccaaattca atatattgca
    33541 aggactgcgt aggcctgatc ggcatagcgc atcaggcagt tttgcatttt tcagctgtct
    33601 gaattttaac cctctggcgg ctttacagta ccttacgcta tactagccac tgaaaatgcc
    33661 ggttcacttt cttcgaatcg gctttcaatg tgtatttcac acaaattaat caacttcccc
    33721 ttccgaggat ctggcctgaa aggtcggata agatatgtta aacagtattt tagtcatact
    33781 ctgcttgatc gctgtaagtg cgttcttctc gatgtctgag atctcacttg ccgcctcacg
    33841 caaaatcaaa cttaaactgc tggctgatga aggcaatata aatgcccaac gcgttctgaa
    33901 tatgcaggaa aatcccggca tgttctttac cgtggtccaa atcggtctga acgcagtggc
    33961 gattctcggc ggtatcgtcg gtgatgcggc attttctcca gcttttcaca gcctgttctc
    34021 ccgctatatg tcggcagagc tctctgagca actgagcttt attctctctt tctcgttagt
    34081 gactggcatg tttatcctgt ttgcggattt aaccccgaaa cgcatcggta tgattgcgcc
    34141 agaagctgtg gctttgcgta tcatcaaccc gatgcgcttc tgcctgtacg tttgcacccc
    34201 gctggtgtgg ttcttcaacg gcctggcgaa cataatcttc cgtattttca aactgccaat
    34261 ggtacgtaaa gatgacatca cttctgatga catctacgcg gtagtggaag ccggtgcgct
    34321 ggcgggcgtg ttacgtaaac aggaacacga gctgattgaa aacgtctttg agctggaatc
    34381 ccgtaccgtt ccgtcttcaa tgacaccgcg tgaaaacgtg atttggtttg atctccacga
    34441 agatgagcaa agtctgaaaa ataaggtggc ggaacatccg cactctaagt tcctcgtctg
    34501 taatgaagat attgaccaca tcatcggcta tgtcgattct aaagacctgc tgaaccgcgt
    34561 gctggctaac caaagcctgg cactgaacag cggcgtacaa attcgcaaca cgctgattgt
    34621 gccggatacg ttaacccttt cagaggcgtt ggaaagtttt aaaaccgcag gagaagactt
    34681 cgcggtgatc atgaacgagt acgcgctggt agtggggatc atcaccctca acgacgtgat
    34741 gaccacgctg atgggcgatc tggtcggtca gggactggaa gagcagattg tcgcccgtga
    34801 tgagaactca tggctgattg acggcggcac tccgattgac gacgtcatgc gcgtgctgga
    34861 tattgacgag ttcccgcagt cgggcaacta cgaaaccatc ggcggcttta tgatgtttat
    34921 gctgcgtaag atcccgaaac gcaccgattc ggtgaaattc gccggctaca aatttgaagt
    34981 ggtggatatc gataactacc gcatcgacca gctgctggtg acccggatcg acagcaaggc
    35041 cacagccctt tcgccaaaac tgcctgacgc taaagataaa gaagaaagcg tcgcgtaacc
    35101 cccataaaca tcaacggctc ctgaatcagg agccgtctta ttactgcata gcacttttta
    35161 ctgcatagca ctttggttaa gccatctctg tttgcagacg cataacctga cggttaactt
    35221 cggacatcac tgacaaatgc agcttatcct tcacttttgg gataagaatc ttacccttat
    35281 cgaattcaaa agcgccaacg tccttgatgt ataatcgtcc acggaacagg atctttacgt
    35341 acttcgccac ctgaagtggg ttgtaccgtt ggaaaatttt cattgttatc tcctgctgag
    35401 tattacgcct gtgcggtgcc acaatcggcc caactattat gaggcgcaaa ttttaatgct
    35461 tagtgactat agactatccg ggcaatgttt ccaccgcgta taactttttt tacctaaagg
    35521 ttacaattat tcagaattat ctttttaccg aagcgcggtc ttcagtataa gcattcattt
    35581 ttcatatgat ttgtgcgctt gaccgcaaac tggcatcaca cttgcgggaa attcgataaa
    35641 tagcacatat gattaaaact cagacccaag tggtcggatc acctgcatat cataagaagg
    35701 aaacaccatg accctacgca agattctggc actcacctgc ctgctgttgc cgatgatggc
    35761 ttccgcacat cagttcgaaa ccggtcagcg agtgccgccg attggcatta ccgatcgggg
    35821 cgagttggtg cttgataaag atcagtttag ctacaaaacc tggaacagcg cgcagttagt
    35881 gggaaaagtg cgagtactgc aacatattgc tggtcgcacc tctgcaaaag agaaaaacgc
    35941 gacgctgatt gaagcgatta aatcagcgaa gttaccgcac gatcgttacc agaccaccac
    36001 cattgttaac accgacgacg cgattccggg ttccggcatg tttgtgcgca gcagtctgga
    36061 gagtaataaa aagctttatc cctggtcgca gtttattgtc gatagcaatg gcgtcgcgcg
    36121 cggtgcctgg cagctggatg aagaaagttc cgctgtagtg gtgctggata aagacggtcg
    36181 cgtgcaatgg gccaaagacg gggcgctcac tcaggaagag gtgcagcaag tgatggacct
    36241 gctgcaaaag ttgctcaaat aatggcaata acataaattc tgcaaaaata attttcccct
    36301 atcgttatct atgggatagg ggaattaagt ctctgagttc ttacgccgaa ctaatactgg
    36361 taacgctagt tgctcttttt taaacgcctc aatggtcgat ttatatttct cgcaaatctt
    36421 ttctttggta atatcactat ctgcggccag attcgcactt actatcgcat ccacttgttc
    36481 ggctaattcg acttgttggc aacccgcatt aaccagaaaa ttataaataa gagagtaatc
    36541 ttcatcctcc aagacaacag aggaaccatt acgaaaacgt tcatcccctc catagatttt
    36601 aagcgcaaag cccaaagatt ctatgactat ggcaaaatta tcatcaatta ttttttcgta
    36661 aatataccat ccatcgcact gtttaattcc acctcctggc ccttgtagtt caaatttacc
    36721 ccgttttaat ttgcttacta tatcatccaa taaagcataa cctaaagaca cgtcaccaat
    36781 cgcctggtga atataacctt taatcctttt cggtatgtca gactgttttt tctgcagaat
    36841 atcccaggca tacaatcgtc tgttttgttc taacggaaat tcacggttct tgaaatccat
    36901 gaatgagtta tactcattta ttgttatggg gatttcttcg tattcaaatc cactgcctcg
    36961 caatgtcatt gtatattcgg caccgttatc tttaaggctg aaactgataa gaaacttatc
    37021 catagcgtta acagtttcat tttttgatga aagcacctga taactacttt tatcaatctg
    37081 agttgcgcaa cgaaagaact ccctcaaagt taacaggcta tcataattcg aaatccaatc
    37141 ttttttctta aatgcatgat ggatgttgtt aaaaaaattg tttgaatgtg tttttgtata
    37201 cgaagacttt acaaataact ccaccgcacc
//
LOCUS       DAZKFZ010000023        36883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000023 DAZKFZ010000000
VERSION     DAZKFZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            102..1685
                     /gene="yegH"
                     /locus_tag="ACHOQW_001106"
     CDS             102..1685
                     /gene="yegH"
                     /locus_tag="ACHOQW_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9572214.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1760..2098
                     /locus_tag="ACHOQW_001107"
     CDS             1760..2098
                     /locus_tag="ACHOQW_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9572215.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2088..2378
                     /locus_tag="ACHOQW_001108"
     CDS             2088..2378
                     /locus_tag="ACHOQW_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9572216.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2431..4284)
                     /gene="asmA"
                     /locus_tag="ACHOQW_001109"
     CDS             complement(2431..4284)
                     /gene="asmA"
                     /locus_tag="ACHOQW_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP9572217.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4306..4887)
                     /gene="dcd"
                     /locus_tag="ACHOQW_001110"
     CDS             complement(4306..4887)
                     /gene="dcd"
                     /locus_tag="ACHOQW_001110"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP9572218.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4979..5620)
                     /gene="udk"
                     /locus_tag="ACHOQW_001111"
     CDS             complement(4979..5620)
                     /gene="udk"
                     /locus_tag="ACHOQW_001111"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9572219.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5938..9256
                     /gene="dgcE"
                     /locus_tag="ACHOQW_001112"
                     /pseudo
     CDS             5938..9256
                     /gene="dgcE"
                     /locus_tag="ACHOQW_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9357..10205)
                     /gene="alkA"
                     /locus_tag="ACHOQW_001113"
     CDS             complement(9357..10205)
                     /gene="alkA"
                     /locus_tag="ACHOQW_001113"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP9572220.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10339..11691
                     /gene="yegD"
                     /locus_tag="ACHOQW_001114"
     CDS             10339..11691
                     /gene="yegD"
                     /locus_tag="ACHOQW_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9572221.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11704..13644)
                     /gene="yegI"
                     /locus_tag="ACHOQW_001115"
     CDS             complement(11704..13644)
                     /gene="yegI"
                     /locus_tag="ACHOQW_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP9572222.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13641..14402)
                     /gene="pphC"
                     /locus_tag="ACHOQW_001116"
     CDS             complement(13641..14402)
                     /gene="pphC"
                     /locus_tag="ACHOQW_001116"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFP9572223.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14399..15058)
                     /gene="yegL"
                     /locus_tag="ACHOQW_001117"
     CDS             complement(14399..15058)
                     /gene="yegL"
                     /locus_tag="ACHOQW_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9572224.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15279..15338)
                     /gene="ibsA"
                     /locus_tag="ACHOQW_001118"
     CDS             complement(15279..15338)
                     /gene="ibsA"
                     /locus_tag="ACHOQW_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP9572225.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15610..15666)
                     /locus_tag="ACHOQW_001119"
     CDS             complement(15610..15666)
                     /locus_tag="ACHOQW_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9572226.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15938..15994)
                     /locus_tag="ACHOQW_001120"
     CDS             complement(15938..15994)
                     /locus_tag="ACHOQW_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9572227.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16274..17521
                     /gene="mdtA"
                     /locus_tag="ACHOQW_001121"
     CDS             16274..17521
                     /gene="mdtA"
                     /locus_tag="ACHOQW_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP9572228.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17521..20643
                     /gene="mdtB"
                     /locus_tag="ACHOQW_001122"
     CDS             17521..20643
                     /gene="mdtB"
                     /locus_tag="ACHOQW_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP9572229.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYSGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20644..23721
                     /gene="mdtC"
                     /locus_tag="ACHOQW_001123"
     CDS             20644..23721
                     /gene="mdtC"
                     /locus_tag="ACHOQW_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP9572230.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23722..25137
                     /gene="mdtD"
                     /locus_tag="ACHOQW_001124"
     CDS             23722..25137
                     /gene="mdtD"
                     /locus_tag="ACHOQW_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572231.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25134..26537
                     /gene="baeS"
                     /locus_tag="ACHOQW_001125"
     CDS             25134..26537
                     /gene="baeS"
                     /locus_tag="ACHOQW_001125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP9572232.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26534..27256
                     /gene="baeR"
                     /locus_tag="ACHOQW_001126"
     CDS             26534..27256
                     /gene="baeR"
                     /locus_tag="ACHOQW_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP9572233.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27447..27779
                     /gene="yegP"
                     /locus_tag="ACHOQW_001127"
     CDS             27447..27779
                     /gene="yegP"
                     /locus_tag="ACHOQW_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP9572234.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27927..29288
                     /gene="yegQ"
                     /locus_tag="ACHOQW_001128"
     CDS             27927..29288
                     /gene="yegQ"
                     /locus_tag="ACHOQW_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP9572235.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29562..29780)
                     /gene="ogrK"
                     /locus_tag="ACHOQW_001129"
     CDS             complement(29562..29780)
                     /gene="ogrK"
                     /locus_tag="ACHOQW_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFP9572236.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(29862..31025)
                     /locus_tag="ACHOQW_001130"
     CDS             complement(29862..31025)
                     /locus_tag="ACHOQW_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP9572237.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPIRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREDSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSIILATGRADIYT
                     ETPVRVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(31025..31504)
                     /locus_tag="ACHOQW_001131"
     CDS             complement(31025..31504)
                     /locus_tag="ACHOQW_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9572238.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDEPLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(31519..33966)
                     /locus_tag="ACHOQW_001132"
     CDS             complement(31519..33966)
                     /locus_tag="ACHOQW_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9572239.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDELGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPIFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRNLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALIGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPLFDWLGEKLQAAWQWFKKLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(33959..34114)
                     /locus_tag="ACHOQW_001133"
     CDS             complement(33959..34114)
                     /locus_tag="ACHOQW_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP9572240.1"
                     /translation="MTFPKNLSVDDLMADVAVVFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(34111..34386)
                     /locus_tag="ACHOQW_001134"
     CDS             complement(34111..34386)
                     /locus_tag="ACHOQW_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP9572241.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(34443..34961)
                     /locus_tag="ACHOQW_001135"
     CDS             complement(34443..34961)
                     /locus_tag="ACHOQW_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP9572242.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(34974..36164)
                     /locus_tag="ACHOQW_001136"
     CDS             complement(34974..36164)
                     /locus_tag="ACHOQW_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFP9572243.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVSSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVNGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCTDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESSDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(36224..36817)
                     /locus_tag="ACHOQW_001137"
     CDS             complement(36224..36817)
                     /locus_tag="ACHOQW_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9572244.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLEAARAKGRIGGRRPKLTTSEWEQVGRLLAAGES
                     RQRVALIFDIGLSTLYKKFPSSATKNKLCHPLANRDK"
BASE COUNT         8299 a        10032 c         9723 g         8829 t
ORIGIN      
        1 taatatgaga taaatagtgc tgcaacattg catttttgcc ccgatttatc catgatcgaa
       61 ttgtgacatt tgtcatacaa cgaataggtt ttgtacttac tatggaatgg attgccgatc
      121 cgtctatctg ggccgggtta atcacgctga ttgtgatcga actggtcctc ggcattgata
      181 acctggtctt tattgccatc ctcgccgaaa aactaccgcc gaagcagcgc gaccgcgcac
      241 gggttaccgg gctgttgctg gcgatgttaa tgcgcctgtt actgctggcg tcaatctcct
      301 ggctggtcac cctgactcaa ccgctgttca gcttccgctc gtttaccttt agcgcccgcg
      361 acttaatcat gctgtttggt ggtttcttcc tgttgttcaa agccacgatg gagctgaacg
      421 aacggctgga agggaaagac agcaataatc ccacacaacg caaaggtgcg aagttctggg
      481 gggtggtgac acaaattgtg gtactggacg ccatcttctc actcgactca gtgattaccg
      541 ccgtcgggat ggtcgaccat ttactggtca tgatggccgc cgtggttatc gctatcagcc
      601 tgatgttgat ggccagcaag ccgttaacgc aatttgttaa cagtcacccg accatcgtta
      661 ttctctgctt aagcttcctg ttgatgattg gctttagcct ggtggcagaa ggtttcggct
      721 tcgtcattcc gaaaggctac ctgtacgctg ccattggttt ctcggtgatg atcgaggcgc
      781 tcaatcagtt ggctatcttt aaccggcgac gttttctttc cgctaaccag acactgcgcc
      841 agcggactac cgaagcggta atgcgcctgc ttagcgggca aaaagaggat gcggaactgg
      901 acgccgaaac cgcgtccatg ctggtggatc atggtaacca gcagatcttt aatccgcagg
      961 aacggcggat gattgagcgg gtacttaatc ttaaccagcg taccgtcagc agcattatga
     1021 cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc ggaagacgag atccgccagt
     1081 tgctggagcg aaatcagcat acgcggctgg tggttactga tggcgatgac gcggaagatt
     1141 tgctcggtgt tgttcacgtt atcgacctgt tacaacaatc actgcgcggc gaaccgctca
     1201 acctgcgggt gttaattcgc cagccgctgg tgttcccgga aaccttgccg ttgttacctg
     1261 ccctggagca gttccgtaat gcccgcacac actttgcttt tgtggtggat gagtttggct
     1321 cggtggaagg gattgtgaca ttaagtgacg tcactgaaac cattgccggt aacttaccga
     1381 acgaagtgga agagatcgac gcccgccatg atattcagaa gaatgcggac ggttcctgga
     1441 cggcgaatgg tcatatgccg ctggaagatc tggtgcaata tgtgccgctg ccgctggatg
     1501 aaaaacgtga atatcacacc attgccgggc tattgatgga atatttgcag cgtattccaa
     1561 aacctggcga agaagttcag gtgggggatt atttgcttaa aacgttgcag gtggaaagcc
     1621 atcgcgtgca gaaggtgcag attataccgc tgcgtaagga tggcgagatg gagtacgagg
     1681 tgtgataaaa catcaaatgc gcaggttcaa actgcgcatt tgatttatac aaaatttagt
     1741 ataatctaag gatggagtaa tgagaaaaaa gctggctttt cttgatacca gtcttgacga
     1801 tttacgcgca tttccagaat cgtctcgaca ggaaattggc tatcaactcg acaggatcca
     1861 acaagggtta aacccatacg actggaaacc tttttcaact atcggccctg gtgtgcgaga
     1921 gattcgtact cgcgatgctg atgggatcta tcgcgtcatg tatgtcgcaa aatttgaaga
     1981 agcggtttac gtattgcatt gttttcaaaa gaaaacacag actaccagtc aatcggatat
     2041 tgatttagcc aaacgacgct ataaagaact tgttcaggag cggaaaaatg aaaattgaaa
     2101 cctttgatag tgtgtgggat gcggtgagtg acacccctga acaagcggaa aatatgcgga
     2161 tccgtgccga gcttgtaacg atcattaata actggattga acaacaaggg tttagccagg
     2221 ctcaggcagc ttctgcttta ggggtcactc aacctcgcat ctctgaactt gccagaggta
     2281 aaatccagat ctttagtatc gataaattga tcaccatgat ggctcatgca ggactacata
     2341 ttcaacgtat tgaagttcaa tatccacatg ctgcttagtg ctataaatac agatggcgac
     2401 aaattgatgc cgccatctat ataacttgtc ttacatcttc tccagcaact tcttcacatc
     2461 tttgccattg cgggaatctt tattccgctc ggcccagtca ttcaggcgac gtttcgcttc
     2521 gtcctgtaga tgtttgcgca gtaactgatc cacttgcaga ctgtaattga gttgctgcca
     2581 gttgccataa acccgcagcg gtactggcgt ttctttgagg aaatcaatca gtttgctttc
     2641 cccgttccag ccacccacga cccgaatatc aaactgggtg tcgcaggttt gatctgccag
     2701 attcaacatg ccttcccctg taagcgccag cacaggcgat tgaccttgca tgtcgtttaa
     2761 cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag cggtcaagac gcgttacgtt
     2821 atcgaagttt tcagcggcct tcacatcacc accattacgt tctaccgctt gctgaatcat
     2881 ctgctggaag ttcatccctt ccatgcgcgt atcggccatt tcgacatgtg cctgtccttg
     2941 ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg gagaaatcac cagccaatga
     3001 catttttccg gtcaacgaaa tcggatagtt aaacgccttc agaatggtgc caatctcaac
     3061 gttttccagc cgtggctgga agtttatccg cggatttatt gatgtcgcgt ccagcgtgcc
     3121 cggcagtgaa acctgtccac cgttaagctt gccctgtagt tgagtaattt ccagcaaacc
     3181 cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc attccgcgcc agcgcacgtt
     3241 actggcctgc aacaaaatat cagccgtaaa gccttgcagt ccctgatagg ccggttcatc
     3301 aatacgcgaa gaaataaccg ggcgcggcaa cgttgattgg ctctgcccct gctgcgcggc
     3361 accgttttca ccattcgcgg tttcattaag cgggatgagg ttgtcaagat tcagttgcgg
     3421 gaattgcagc ctcagctgcc attccggttt ctctgtcagc gtgacctgtg cttgcccgct
     3481 cagcgtacta tcattggcgg tcaaactaat ttggttaaat gaaaggcgtt tacgtgactc
     3541 ctgccactgg gcctggaaac tcccctgccc ctgaatacct tgttttggta aatcggcacc
     3601 ctgcaactgc cagttaattt gttcaatagc cgccgttaaa tcatgcggat aatcagaagc
     3661 atctaccgta ccgttaaggg atatcgtgag atcgcgctga tcgcgattaa cccgcccgga
     3721 gaactcaaat gagccacgat gttggggatc ttgttccatt tgcaggcgga tattgcggat
     3781 tgtcacctgc tcgtcatctt catgctggaa caccagcacg ctgtccgcca ccttaagact
     3841 ggatatatca aacgaccatc cgcgatcgtc tgacagatcc ggcaaggtat tgtcgcgcgg
     3901 tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc ggcgtcagtt ggatcactgc
     3961 cccttttagc atcacctgct taacgctcag ttgatgactc agtagtggta aaagcgccac
     4021 gtccaggcgc atgttgtcgg cgcgaaccag tggctggctc gcgccctggg cggtgagaga
     4081 cattcgcccg gagaggatac taagctgcgg ccagacgtgc caacgcagtg gcccgtcgag
     4141 ctgcaattga taaccgctac gtgcagcaac ttgcttgacc atatagtcgc ggaaatcatt
     4201 cggattcacc agcaacacta acgcagataa cccggccacc agcacgacca ggagtatcat
     4261 cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc ctgaattagt ctttatcgat
     4321 tcggctggct accgcgccct gctggttgcg atacttcgca tcttcacggc ggttgtaagg
     4381 tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca ccaattaaca tgcccggacg
     4441 cagcgccagc ggcagcttac cggagttgta gaactccagc acaatgcaac cagaccagcc
     4501 cggatcgata cggtgcgcgg tgacgtgcac catcagcccc agacgcgcca gtgaggaacg
     4561 cccgtccagc cagcccacca gatcggctgg cagcgtcacc gactccagcg tcaccgccag
     4621 cgccagctct cctgggtgaa gatagaacgc ctcgctttca tcgagaacga tctcatcgct
     4681 catcacgcgg tcaagcgcgg cgctcacttc atctttggga ccgctcagat cgataaacgc
     4741 tgccgtgtga ccacggaagg tacgaaattt attgcccagg cgtacatcca ccgtcgcgcc
     4801 gttaatacgc tccactggcg gacgtgggtt gatcgacaaa cggccttcat caagccaggc
     4861 ttcaatatct cggtcacaca gacgcatggc attttctcct ttcgcgcatc actcccttaa
     4921 cgccaattac gtcaagggca taccaggtta tcactgaacg gtacacaatt tatcaagctt
     4981 attcaaagaa ctgacttatt ttcgctttca atatatcgat cgcgatgcgg tttttcccgc
     5041 cgcgcggcac gataatgtcc gcatattgtt tagaaggctc aatgaattgc aggaacatcg
     5101 ggcgcacggt tttttgatat tgcgccatca cggaatccat tgaacgccca cgctcgttaa
     5161 cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg ggtatcaacg aaaatggaga
     5221 agttaagttc gtcacgcaaa cgtgcatccg tcagcaacaa aatgccttcg agaatgatga
     5281 ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt atgttcaaca tagctgtaaa
     5341 ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa atgctcaagc agcaggctgt
     5401 gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg ttcttccatc gacagatggc
     5461 tttgatcttt gtaatagcag tcttcgggaa ttacgccgat gtgttcatca ccgacttgct
     5521 cacgtaattc acgataaagg gtactggcaa taagactctt gccggaagcc gatgcgccag
     5581 cgataccgat aatgacgcac tgatgagact gatcagtcat atatttagcg acctgattaa
     5641 cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg caattatagg gatttcatcc
     5701 gcctgatacc agtcgaatag cgttgccgcg cgctcagagt taattgttga cgaagaattc
     5761 ccggtggcaa attacgttga tcagttttat acaaggtaaa aattgttata cgcagttgcg
     5821 caaattatcc gcctttacgt cactttatga gcaattcgca tataaaatgt aaaacttttg
     5881 tactagcata aacacagaaa cgaatactgg cgacctggcc ttgcggataa agcggtaatg
     5941 agcaaacaat cacagcatgt attaattgcc ctgccccacc cgctgcttca cctggtcagt
     6001 ttaggtttag tctcgtttat cttcaccctt ttctcgcttg agctttcgca gtttggcacc
     6061 caactcgccc cactgtggtt cccgacgtcc atcatgatgg tggcgtttta tcgccatgcc
     6121 gggcgcatgt ggccagggat tgcgctgagc tgctcgctgg gaaatatcgc cgcatccatc
     6181 ctgctttttt ccaccagctc gctgaacatg acctgcacga ccatcaatat tgttgaagcc
     6241 gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt ataacccctt gcaaaatctg
     6301 gctgactggc tgcgtctggc actcggcagc gccattgttc cacctctgtt ggggggtgtt
     6361 ctggttgtcc tgctgacgcc cggagacgat cctcatcagg gcatttttga tatgggtact
     6421 gtcagaatcc atcggcgctc tggcactggt gccgctggga ttgttattta aaccacacta
     6481 tctgctgcgc catcgcaacc cacggttgct ttttgagtcg ctgctcacgt tagccatcac
     6541 actgacgtta agctggcttt cgatgctgta tctgccgtgg ccttttactt tcattattgt
     6601 gctgttgatg tggagcgccg tgcgcctgcc acgaatggaa gcctttttga tcttccttac
     6661 cacggtgatg atggtgtcgc tgatgatggc cgcggatccc tccctgcttg ctacgccgcg
     6721 tacgtacctg atgagccata tgccgtggct accgtttttg ctgatcctgc tgcccgccaa
     6781 catcatgacg atggtgatgt atgcctttcg tgcggaacgc aaacacattt ccgaaagcga
     6841 aacccgtttt cggaacgcca tggaatattc cgccatcggc atggcattag tgggcaccga
     6901 gggacaatgg ctgcaatcca acaaagcact ctgccagttt ctcggttaca gtcaggaaga
     6961 gctgcgcgga ctcacctttc agcaactgac ctggccggag gatctcaata aagatctcca
     7021 acaggttgaa aagctgataa gcggtgaaat aaacacctat tcaatggaaa aacgttacta
     7081 caaccgcaat ggcgatgttg tctgggcgtt gcttgccgtc tctctggtgc gccacacgga
     7141 tggcacgccg ctctatttta tcgctcagat tgaagacatt aacgagctaa aacgcaccga
     7201 acaggtgaat cagcaactga tggaacgcat cacgctggct aacgaagcgg gcgggattgg
     7261 catctgggag tgggaactga agccgaatat ttttagctgg gataagcgga tgttcgagct
     7321 gtatgaaatt cctccgcata tcaaaccgaa ctggcaggtg tggtacgagt gcgtgctgcc
     7381 ggaagatcgc caacacgccg aaaaagtgat tcgtgattcg ttgcaatcac gctcgccctt
     7441 taaactggaa tttcgcattg ccgtgaaaga tggcatccgc catatccgcg ccctcgccaa
     7501 ccgggtactg aataaagaag gcgaagtcga acgcctgctc ggcattaata tggatatgac
     7561 tgaggttaaa cagcttaacg aggcattgtt tcaggaaaaa gagcgcctgc acattacgct
     7621 ggactccatc ggcgaagccg tggtctgtat tgatatggcg atgaaaatta cctttatgaa
     7681 tccagtggcg gagaagatga gcggctggac gcaggaagaa gcgttaggtg ttccgctcct
     7741 gacggtgttg catattactt ttggcgacaa cggaccatta atggagaaca tttacagtgc
     7801 cgacacctca cgttccgcga tcgaacaaga tgtggtgttg cactgtcgga gcggcggcag
     7861 ctacgacgtg cattacagta ttacgccgtt aagtactctg gacggcagca atataggctc
     7921 ggtactggtg attcaggacg ttaccgaatc acgcaaaatg ctgcgccagc tgagctacag
     7981 cgcctcccat gatgcactga cgcatctcgc caaccgcgcc agttttgaga aacaactgcg
     8041 tatcctgctg caaacggtaa acagtacgca tcagcgacat gcactggtgt ttatcgatct
     8101 tgatcgcttt aaagcggtga atgacagcgc cgggcatgcg gcgggcgacg ctttgctgcg
     8161 cgaactggcg tcgttgatgc tgagtatgct gcgctccagt gacgtgctgg cgcggctcgg
     8221 cggtgatgaa tttggtctgc tgctgccaga ctgcaatgtt gaaagcgctc gttttatcgc
     8281 tacacgtatt atcagtgctg tgaatgacta tcactttata tgggaaggcc gggtacatcg
     8341 ggtaggtgcc agcgccggga ttaccttgat cgatgacaac aatcatcagg cggcagaagt
     8401 gatgtcgcag gctgatatcg cctgttatgc ctccaaaaat ggtggccggg gccgggtgac
     8461 ggtttacgaa ccgcagcaag ctgccgcaca tagcgaacgg gcggcgatgt cgcttgatga
     8521 acagtggcgg atgattaaag agaatcagtt gatgatgctc gcccacggtg tcgcttcgcc
     8581 acggatcccg gaagcgcgta atttgtggct gatttcactt aagctctgga gttgcgaagg
     8641 cgagattatt gatgaacaaa catttcgtcg tagcttcagc gatccggcgc ttagccatgc
     8701 tcttgaccgc cgggtattcc acgaatttct ccagcaggcc gcaaaagcgg ttgccagtaa
     8761 aggcataagc atcgccctcc ccctttccgt tgccggtttg agtagcgcca cgctggtgaa
     8821 tgatctgctt gagcagctgg aaaatagccc tctaccacca cggttattac atctgattat
     8881 tccggcagaa gcgattttcg atcacgcaga aagcgtgcaa aaactgcggc tggggggatg
     8941 ccggatcgta ttcagtcagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa
     9001 tatggcagat tacctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat
     9061 ggatgagatg ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat
     9121 cgccgggcca gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct
     9181 gatttatggt gaggtgattg ccgatgccca accgctggat ttgctgatga atagtagtta
     9241 tttcgcgatt aactgaggga gcgtttgccc attgcctgat gcgacgctaa cgcgtcttat
     9301 caggcctaca aatcgctcat tccgcaaggc gctcgcaccg catccggcga ccaacgtcat
     9361 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     9421 ggcttccagc gctcggcata acggcggatt tgcgccggtg tcattcccgg aaatcgctgt
     9481 ttaatcagat aatcatccgg caggaaaaca tctttcgcct gccagccacg caaagcaaaa
     9541 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     9601 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     9661 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtctgctgct
     9721 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     9781 taaagctgta ccactttggc agtcaatttt gccgccatcg ccacgctcac cagttggcct
     9841 aaaatcgccc gcacgccttg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     9901 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     9961 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaatc tgcacttaaa
    10021 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
    10081 cccaccgcca gactacgggc ataataatcg tcagcgaccg tttccacacc actcactgca
    10141 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcagggta
    10201 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gctgcgcttt
    10261 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
    10321 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagta
    10381 gcggtcatgc gtgacggtaa accgcacttg ctaaaaatgg aaaacgacag cacgctgctg
    10441 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
    10501 gatgttccgg cagacgacga tgaaacgcag gcactgttgc gtcgggcgat tcgttataat
    10561 cgcgaagaag atatcgatgt tacggcgaaa agcgtgcagt tcggtctttc ctcactggca
    10621 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
    10681 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
    10741 ttgcacattc gccagcaggc gcaagcgcag ctgccagaag cgattactca ggcggtgatt
    10801 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcaaggg
    10861 attctggaac gcgcggcgaa gcgtgccgga ttcagggacg tggtattcca gtacgagccg
    10921 gtagcggctg ggctggatta cgaagccacc ttacaggaag aaaaacgggt actggtggtg
    10981 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
    11041 cgtctcgatc gtgaagccag cctgctgggt cacagtggtt gccgtattgg cggtaacgat
    11101 ctggatatcg cactggcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
    11161 gaaaaaggca tcgccctgcc gattctgccg tggtggaatg cggttgccat caacgacgta
    11221 cctgcgcaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
    11281 gatgcccgcg aaccggaaaa agtggccctg ctacagaaag tctggcgtca gcgtttaagc
    11341 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgcgc tttcaagcgt agcggaaacc
    11401 cgcgcctcac tgccgtttat cagcgatgaa ctggctacgc tgattagcca gcaagggctg
    11461 gaaagcgccc tcagccagcc actggcgcgg attcaggaac aggtgcaact ggcgctggat
    11521 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gaagcgcccg ttctccgctg
    11581 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
    11641 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tgtttcgtta attgaaaaat
    11701 ggctcagcag cggcttaaat ctgcctgcgc ctgagccagt ttttctgccg cctgacgtaa
    11761 cggttccatc aacggcatgg tccgtgctgg agcatgaagg cggaatcgct gcaactctgt
    11821 cgcgccgaca gtcaacgccg attccagccg atgtcgtttg gcggccattt tcgccattac
    11881 cgcttgtctt tctgcaggcg ttaccgcttc gttcggcctg aacacaaaac ggcgttcaca
    11941 gctcgctttc cagtcgataa ccgcctgggt cagatgatca ccaaaccctt taacttgctt
    12001 aaccccacga cgggtaacat ccgcggctgt ttcaatacca aaagaccgta acgccgcttt
    12061 acgcgcaggg ccaacgccgg gaatagaggc agcatcaata aaaaatccct ccagaaactt
    12121 ctgcttctgc cgttcccttg cagtgtcatg aagtgctgcc agagcgcgtt tctcctcttc
    12181 cggtaacccg agaatttcgt ccttcatttt ttcgagcatc gcccgtttgg cgataaaacc
    12241 ttccagcccg cccaactgtt ggatctggct gactaaatgg tcatagtcca ttttcgcgcg
    12301 attaaatgcc tcacggcgtt gctggatttc tgctttataa actttgcttg tcagactgcc
    12361 aataatccag atagccgcca gcacaggaac caaaataata taacgcggtt ctgcctgaag
    12421 gccgcaaagc aacagcgata acgctgacag tgcgatctca atcagaatga tgtattcgcg
    12481 ccgtaacagg cctaaaggaa gcggcctgcc agtcggttgg aaatgatcgg gtaatggcaa
    12541 ttgcaatgct ggcggtgcta ctgacgccat caccatcgcc cagactttcg ccagcacaaa
    12601 atcaccactg gtggtaatga cctcttcgcc gagatcaata aaatagataa cgccttgatt
    12661 atccagcgca caccacgggc agtcagtcaa atgaccgggg taaacatgca ttgccgaaac
    12721 ggtacatttc tttaactgtt ggcgtagtaa atccagtgcc gctacccacg cttttgccgt
    12781 cggacgcgcg gttgccacgc cactttccgt gaatgcctgc tgaaacatgg cttcaacatc
    12841 gcccggtaac atcgacagcg gaatcgatcg tggcggcggt tttaaaccac gtcgctggtt
    12901 gtctgacgcg taggcataac ggaaatgggc aatatccgtc tccagcgcat tacccgcatc
    12961 agagataagc ggcacaccag aataaggatg ccgcccacca aacaagacgt gaaaaatcag
    13021 caacgcaagg ccaaaattat cgtgattcgc ggtacgttca aaaccgacaa atgatggcaa
    13081 ggtttgtagc tctggcggcg taaaatgcga cacgccgact tcgcataaat gcagtgtgcc
    13141 attggcgtta atctgaaagg agtcactatc gatcaacacc actttgctgt cgcgacctac
    13201 cataaagctg ttctggttta cgtcccccac gacgtgcccg tgctcgtgaa ccgtagcaaa
    13261 agatgaagca atattgcgcg caacatagag tagaaaatcc cacgcacaat gaggataact
    13321 ctggcgacga tgtgccgggc tatagatcat atgaatcggt tctttaccag aaacttttgg
    13381 catcataaaa ccgataactt ttccgcctcg tccaccgtga agcgttgcct gcggccaggc
    13441 gacataattc aacaactgcg cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt
    13501 taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta tcgacaaact cattgatatc
    13561 ataaaccgcg ccttcgccac ctttgcccag ttcacggcct ggcgtaacgc actcaccagt
    13621 agcagtatat aaagtgggtt tcatggtaac cacagcgcca gtgtcaacgt tttatcatca
    13681 tcggtacgct cgtttaccgc tgggctcgac aaaaactgct ttaacaattc aggtaataaa
    13741 tcgagttgtt cctgcgttgc tgccgccagt ccattgaaaa acggggtaaa gaaaggcaca
    13801 tgcggagaat tatccagcat atttaacgcc agtcgctgaa taccatccgt aaatgctgca
    13861 actttatgcg cacgctcagt gctggtgaaa gtttccagtc tggaaacgac atcttcatcg
    13921 gtaatgaaat gcgtcatatt ggcatattca cccaccatcg gcgtaagcgg taattgcaga
    13981 ccgtgaccaa aatccaccac cacgccgcca tcgccaatct gcataatcaa ggtgccgtta
    14041 gccgatgaaa tcaatcccag aaacgtgcag gcaaaatcgc gaactgctaa ttctttagct
    14101 tccgcctcgg caaaaagtcg ctgacgaacg gtcagcacaa tatccgtggc gagaatatca
    14161 ttgagtccaa attcaccgtg ctgcactttt tgcgacacat aagccatcgc ttcattgatg
    14221 gcaagcatcg cgccttcgcc accctgcgag acactgcctg cgccatcagc aaggaacatc
    14281 accagtaatg gctgctggtc atttagccag gcaacctgca tctggcaggc atcctgacaa
    14341 ggtaaatcag cgctaatgtg cgacgttcca acagcggatg cataaaccag acgccagctc
    14401 acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc gtggagcggg
    14461 aaaccgaacg cagcgaactg gataaccagc taaacagttc acggaattgt aatccctgca
    14521 gtggcaaagg ctgacgaacg ctgatttgtg ccagagtctt aatatccgcc ccctgaacac
    14581 cgatggaaaa gaaggcaaac ttcttatctt cttccccctg aaacactttg ttggcggcag
    14641 cctgccactc atcggttggt gcaccatcgg tgatcaggaa aatccacggg cgataatacg
    14701 aaataccatt tgcacgatac tcacgtttcc gctcctctac catatccagg gctttggtaa
    14761 tggcggcacc cattggtgta tccccctggg caaacaggat gggcgggaaa aaattagcgg
    14821 cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatacca agttccactc
    14881 tttttaaggc cagcgggtcg gcaagcagct catcgcgaaa ggtaaccaat ccggcgttaa
    14941 gttcattaat gggtctaccg ttcatcgaac cagaaacatc cagcagcaaa atgcaggggc
    15001 aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa
    15061 gaaatccttt cttagagaat tattgaggtc cggtatcata ccgattgcaa taatgaaata
    15121 agagcaaacg aagaaacttt cagaattagg cccggcggcg cagaaaattt tgctattcgc
    15181 ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa tttcaggagt
    15241 gagggttagg gaaaggtttc cccctccccc tggtgttctt agtaagcctg gaagctaatc
    15301 actaagagta tcaccagtat gatgacgtgc ttcatcataa cccttttctt atttaaggcc
    15361 ccttcctcgg gaggggcttt cccgtttcag catcccgctg aaatcgtcgg cttacctcct
    15421 ttcgccatgc atgcagtcta tcgctgactt gcggataaaa tagtttccag tgtgattatt
    15481 gcgtaacgtc tcgcaaagaa tatttgctgg caggatcgca gactgcaaag cctgcagatt
    15541 gacaatctta tcgtgaaggc atactttcag gagtgagggt agagcggggt ttcccccgcc
    15601 ctggtagtct tagtaagccg ggaagcttat gactaagagc accacgatga tgatccactt
    15661 catcatgacc ctttccttat ttaaggcccc ttcctcggga ggggctttcc cgtttcagcg
    15721 tcccgctgaa atcgtcggct tacctccttt cgccctgaat tcagtctatc gctgacttgc
    15781 ggataaaata gtttcctctg tgattattgc gtaacgtctc gcaaagaata tttgctggca
    15841 ggatcgcaga ctgcaaagcc tgcggattga cattctgaac gtgaaggcat actttcagga
    15901 gtgagggtag agcggggttt cccccgccct ggtagtctta gtatgctggg tagctaatca
    15961 ctaagagcac cacgatgatg agtatcttca tcatgaccct ttccttattt atggcccctt
    16021 cctcgggagg gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt acctcctttc
    16081 gccatgcaag cagtctatcg ctaacgcgta gataaaatag tttcctgtgt tattactgga
    16141 tgcgtgctcg caaatgtgcc cgccattcag acgattcccg atagtgtttc ataattcctc
    16201 catttttctc ccttattggc tggctacact agtatcattc cgcgaaacgt ttcaggaaga
    16261 gaaactctta acgatgaaag gcagttataa atcccgttgg gtaatcgtaa tcgtggtggt
    16321 tatcgccgcc atcgccgcat tctggttctg gcaaggccgc aatgactccc agagtgcagc
    16381 cccaggggcg acgaaacaag cgcagcaatc gccagcgggt ggtcgccgtg gtatgcgttc
    16441 cggcccatta gccccggttc aggcggcgac cgccgtagaa caggcagttc cgcgttacct
    16501 caccgggctt ggcaccatta ccgctgctaa tactgttacg gtgcgtagcc gcgtggacgg
    16561 tcaactgatg gcgttacatt tccaggaagg ccagcaggtc aaagctggcg atttactggc
    16621 agaaattgac cccagccagt tcaaagttgc attagcacaa gcccagggcc aactggcaaa
    16681 agataaagcc acgcttacca acgcccgccg tgatctggcg cgttatcagc aactggcaaa
    16741 aaccaatctc gtctcccgcc aggagctgga tgcccaacag gcgctggtca gtgaaaccga
    16801 aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg cagctgcaac tcgactggag
    16861 ccgtattacc gcaccagtcg atggtcgcgt tggtctcaag caggttgatg ttggtaacca
    16921 aatctccagt ggtgatacca ccgggatcgt ggtgatcacc cagacgcatc ctatcgattt
    16981 gctctttacc ctgccggaaa gcgatatcgc taccgtcgtg caggcgcaaa aagccggaaa
    17041 accgctggtg gtagaagcct gggatcgcac caactcgaag aaattaagtg aaggcacgct
    17101 gttaagtctc gataaccaaa tcgatgccac taccggtacg attaaagtga aagcacgctt
    17161 taataatcag gatgatgcgc tgtttcccaa tcagtttgtt aacgcgcgca tgttagtcga
    17221 caccgaacaa aacgccgtag tgatcccaac agccgccctg caaatgggca atgaaggcca
    17281 ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa catctggtga cgccgggcat
    17341 tcaggacagt cagaaagtgg tgatccgcgc aggtatttct gcgggcgatc gcgtggtgac
    17401 agacggcatt gatcgcctga ccgaaggggc gaaagtggaa gtggtggaag cccagagcgc
    17461 caccactccg gaagagaaag ccaccagccg cgaatacgcg aaaaaaggag ctcgctcctg
    17521 atgcaagtgt tacccccgag cagcacaggc ggcccgtcgc gcctgtttat tatgcgtcct
    17581 gtggccacca cgctgctgat ggtggcgatc ttactcgccg ggattatcgg ttatcgcgcc
    17641 ctgcccgttt cggcgctgcc ggaagtggac tatccgacca ttcaggtggt cacgctctac
    17701 tcaggtgcga gcccggatgt catgacctct gccgttaccg cgccgctcga acgccagttc
    17761 gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt ccggcggtgc gtcagttatc
    17821 actttacagt tccagctaac attaccgctg gatgtcgccg agcaggaagt gcaggccgcg
    17881 attaacgctg cgaccaactt gttgccgagc gatctgccta acccgccggt ttacagcaaa
    17941 gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca cctcaacagc catgccgatg
    18001 acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga aaatctcgca gatttccggc
    18061 gtcggcctgg tgacgctttc cggcggccag cgtccggctg ttcgcgtcaa acttaacgct
    18121 caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc gcaccgccat taccggcgct
    18181 aacgttaact cggctaaagg tagccttgac ggcccttccc gtgcggttac gctttccgct
    18241 aacgaccaga tgcaatccgc cgaagagtat cgccagctaa tcatcgccta ccagaacggc
    18301 gcgccgattc gtctgggcga tgtcgcaact gtagagcaag gtgcagaaaa cagctggctc
    18361 ggcgcatggg cgaataaaga acaggccatt gtgatgaatg ttcagcgcca gcccggtgct
    18421 aacattatct ccaccgccga cagcatccgg cagatgctgc cacagctcac cgagagtctg
    18481 ccgaaatcgg tgaaggtgac ggtactttct gaccgtacca ccaatatccg cgcatccgtc
    18541 gatgatactc agtttgaatt gatgatggct atcgcgctgg tagtcatgat tatctacctg
    18601 tttttgcgca atattccggc gaccatcatt cccggcgttg cagtgccgct gtcgttaatc
    18661 ggcaccttcg cggtcatggt gtttctcgat ttttcaatca ataacctgac gctgatggcg
    18721 ttaactatcg ccaccgggtt cgtggtcgat gacgccatcg tggtgatcga aaatatttcc
    18781 cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc tcaaaggcgc aggtgaaatc
    18841 ggctttacca ttatctcgct gaccttctcg ctgattgcgg tgttgatccc actgctgttt
    18901 atgggcgata tcgtcgggcg actgttccgc gaatttgcta ttaccctggc ggtagcgatt
    18961 ttaatctcag ccgtggtgtc gctaaccctg acaccgatga tgtgcgcacg gatgctcagc
    19021 caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct cggaaaaaat gttcgacagg
    19081 ataatcgccg cctatggtcg tggactggcg aaagtgctga atcatccgtg gctgacctta
    19141 agcgtggcgc tcagcacgct gctgcttagc gttctattgt gggtgttcat tccgaaaggt
    19201 ttcttcccgg tacaggataa cggcattatt cagggcactt tgcaggcacc gcaatccagc
    19261 tcctttgcca atatggccca gcggcaacgc caggtcgcgg acgtgatttt gcaggatccg
    19321 gcagtgcaaa gtctgacctc atttgttggc gttgatggca ctaacccgtc gctgaacagt
    19381 gcgcgtttgc agatcaacct caaaccgttg gatgaacgtg atgatcgggt gcaaaaagtc
    19441 atcgcccgtc tgcaaacggc ggtggataaa gtgccgggcg tcgatctctt cctgcaacca
    19501 acgcaggacc tgaccatcga tactcaggtc agccgcaccc agtaccagtt taccttgcag
    19561 gccacgtcac tggatgcgct cagtacctgg gtgccacagt tgatggaaaa actccagcag
    19621 ctgccacaga tttctgatgt ctccagcgac tggcaggaca aagggctggt ggcgtatgtc
    19681 aatgttgatc gcgacagcgc cagccgtctg gggatcagca tggcggatgt cgataacgcc
    19741 ctgtacaacg cgtttggtca gcgactgatt tccactattt atactcaggc taaccagtat
    19801 cgtgtagtgc tggagcacaa taccgaaaat accccaggcc tcgcggcgct ggataccatt
    19861 cgcctgacca gcagcgacgg cggcgtggtg ccgctaagct caattgccaa aattgagcag
    19921 cgttttgcgc cgctttccat caaccatctg gatcagttcc cggtaacgac catctccttt
    19981 aacgtgccgg ataactattc gctgggcgat gcggtgcagg caattatgga caccgagaag
    20041 acgctgaatc tgccggtgga tatcaccacg caattccagg gcagcaccct cgccttccag
    20101 tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg tggtggcgat gtatatcgtg
    20161 ctcggcattc tgtacgagag ctttattcac ccgatcacca ttctctcgac gctacccacg
    20221 gcaggtgttg gcgccctgct ggcgttaatg attgccggta gcgaactgga tgtgattgcg
    20281 attatcggca ttattttgct gatcggtatc gtgaagaaga acgccatcat gatgatcgac
    20341 ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc gcgatgcaat ctaccaggct
    20401 tgtctgttgc gttttcgtcc gatcctgatg accaccctgg cggctctgct tggcgcgctg
    20461 ccgctgatgt tgagtaccgg ggtcggcgcg gaactacgtc gtccgttagg tatcggcatg
    20521 gtcggcggcc tgattgtcag ccaggtgctg acgctgttca ccacgccggt gatttatctg
    20581 ctgtttgacc gcctggcatt gtggaccaaa agtcgctttg ctcgccatga agaggaggcg
    20641 taagtgaagt tttttgccct cttcatttac cgcccggtgg cgacgatttt actgtcggtc
    20701 gccattaccc tgtgcggcat actgggtttc cgtatgctgc cggtcgcccc gctgccgcag
    20761 gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg gtgcgtcgcc ggagacaatg
    20821 gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc gcattgccgg ggtcagtgaa
    20881 atgacctcca gcagttcgct cggcagcacg cgtattattt tgcagtttga ttttgaccgg
    20941 gatatcaacg gtgcggcgcg tgatgtgcag gcggcgatca acgccgcaca aagtctgctg
    21001 cccagtggga tgcccagccg cccgacttat cgcaaagcga acccgtcgga tgcgccaatt
    21061 atgatcctca cgctaacctc cgatacttat tcgcagggtg aactgtacga tttcgcctcg
    21121 acgcagctgg ctccgacgat ttcgcaaatc gacggtgtcg gtgatgtcga tgtcggcggc
    21181 agctcgctgc ccgccgtgcg cgtcgggctg aacccgcagg cgctgtttaa tcagggcgtg
    21241 tcgctggacg acgtacgcac tgccatcagc aatgccaacg tgcgtaaacc gcagggggcg
    21301 ctggaagatg gcactcaccg ctggcagatc cagaccaacg atgaactgaa aaccgccgct
    21361 gaatatcagc cgctgattat tcactacaac aacggcggcg cggttcgtct gggcgatgtg
    21421 gcgacggtga ccgactcagt gcaggatgtg cgcaacgccg ggatgaccaa cgccaaaccg
    21481 gctattttac tgatgatccg caaattgccg gaagccaata ttatccagac ggtagacagc
    21541 atccgggcaa aattaccgga gttgcaggaa accattccgg cggcgattga tctgcaaatt
    21601 gcccaggatc gctcccccac cattcgcgcc tcgctggaag aagtcgagca aacgctgatt
    21661 atctcggtgg cgctggtgat tctggtggtc tttttattcc tgcgctcggg tcgcgccact
    21721 attattcccg ccgttgcggt gccggtttcg ctgattggta cgtttgcggc gatgtacctg
    21781 tgcggattca gtctcaataa cctttcgtta atggcgctca ccatcgctac tggtttcgtg
    21841 gtggatgacg ccatcgtggt gctggaaaac attgcacgtc atctggaagc gggaatgaaa
    21901 ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt ttacggtgct gtcgatgagt
    21961 ctgtcgctgg tggcggtgtt cctgccgctg ctgttgatgg gcggattgcc gggccgactg
    22021 ttacgcgagt ttgccgtgac gctttctgtc gccattggta tttcgttgct ggtttctctg
    22081 acattaacgc caatgatgtg tggctggatg ctgaaagcca gcaagccgcg cgagcaaaag
    22141 cgactgcgtg gttttggtcg catgttggta gccctgcaac aaggctatgg taagtcactg
    22201 aaatgggtgc tcaatcatac ccgtctggtg ggcatggtgc tgcttggcac cattgctttg
    22261 aatatctggc tatatatctc gattccgaaa accttcttcc cggagcagga cactggcgtg
    22321 ttgatgggcg ggatacaggc ggatcagagt atttcgtttc aggcgatgcg cggtaagttg
    22381 caggatttca tgaaaattat ccgtgacgat ccggcagtgg ataatgtcac cggctttacc
    22441 ggcggttcgc gagtgaacag cgggatgatg tttatcaccc tcaagccacg cgacgaacgc
    22501 agcgaaacag cgcagcaaat tattgaccgt ctacgcgtga agctggcgaa agaaccgggg
    22561 gcgaatctgt tcctgatggc ggtacaggat attcgcgttg gcggacgtca gtcgaacgcc
    22621 agctaccagt acacgttgtt atccgacgac ctggcggcac tgcgcgaatg ggagccgaaa
    22681 atccgcaaaa aactggcgac attgccggaa ctggcggacg tgaactccga tcagcaggat
    22741 aacggcgcgg agatgaatct ggtttacgat cgcgacacca tggcacggct gggaatcgac
    22801 gtacaggccg ccaacagcct gttaaataac gccttcggtc agcggcaaat ctcgaccatt
    22861 taccagccga tgaaccagta taaagtggtg atggaagtgg atccgcgcta tacccaggac
    22921 atcagcgcgc tggaaaaaat gttcgtaatc aataacgaag gaaaagcgat cccgctgtcg
    22981 tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg tgaatcatca gggattatcg
    23041 gcagcctcga ccatctcgtt caacctgccg accggaaaat cgctctcgga cgccagtgcg
    23101 gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga cggtgcgcgg cagttttgcc
    23161 ggcacggcgc aggtgttcca ggagacgatg aactcacagg tgatcctgat tatcgccgcc
    23221 atcgccacgg tgtatatcgt gctgggtatc ctttacgaga gttacgtaca tccgctgacg
    23281 attctctcca ccctgccctc ggcgggcgtt ggagcgctgc tggcgctgga gctgttcaat
    23341 gccccgttca gcctaatcgc cctgataggg atcatgctat taatcggcat tgtgaagaaa
    23401 aacgccatta tgatggtcga ttttgcgctt gaagcccaac ggcacggtaa cctgacgccg
    23461 caggaagcta ttttccaggc ctgtctgctg cgttttcgcc cgattatgat gactaccctg
    23521 gcggcgctgt ttggcgcgct gccgctggtg ttgtcgggcg gtgacggctc ggagctgcgg
    23581 caacccttgg ggatcaccat tgtcggcggt ctggtgatga gtcagctcct gacgctgtat
    23641 accacgccgg tggtatatct ctttttcgac cgtctgcggc tgcgtttttc gcgtaaatct
    23701 aaacaaacgg taaccgagta aatgacagat cttcccgaca gcacccgctg gcaattgtgg
    23761 attgtggctt tcggcttctt tatgcagtcg ctggacacca ccatcgtaaa caccgccctt
    23821 ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata tgcacatggt cattgtctct
    23881 tatgtgctga ccgtggcggt gatgctgccc gccagcggct ggctggcgga caaagtcggc
    23941 gtgcgcaata ttttctttac cgctatcgtg ctgtttactc tcggttcact gttttgcgcg
    24001 ctttccggca cgctgaacga actgttgctg gcacgcgcgt tacagggcgt tggcggtgcg
    24061 atgatggtgc cggtcggcag gttgacggtg atgaaaatcg taccgcgcga gcaatatatg
    24121 gcggcgatga cctttgtcac gttacccggt caggtcggtc cgctgctcgg tccggcgctc
    24181 ggcggtctgc tggtggagta cgcgtcgtgg cactggatct ttttgatcaa cattccggtg
    24241 gggattatcg gtgcgatcgc cacattgatg ttaatgccga actacaccat gcagacgcgg
    24301 cgctttgatc tttccggctt tttattgctg gcggttggca tggcggtatt aaccctggcg
    24361 ctggacggca gtaaaggtac aggtttatcg ccgctggcga ttactggcct ggtcgcagtt
    24421 ggtgtggtgg cactggtgct ttatctgctg cacgccagaa ataacaaccg cgctcttttc
    24481 agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc tggcggggag ctttgccgga
    24541 cgtatcggta gcggcatgtt gccctttatg acgccggttt tcctgcaaat tggccttggc
    24601 ttctcaccgt ttcatgccgg actgatgatg atcccgatgg tgcttggcag catggggatg
    24661 aagcggattg ttgtacaggt ggtgaatcgc tttggttatc gtcgggtact ggtagcgacc
    24721 acgctgggtc tgtcgctggt caccctgttg tttatgacta ccgctctgtt gggctggtac
    24781 tacgttttgc cgttcgtcct gtttttacaa gggatggtca actcgacccg tttctcctcc
    24841 atgaacaccc tgacgctgaa agatctcccg gacaatctgg cgagcagcgg caacagtctg
    24901 ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca ccatcgccgg gctgttgctg
    24961 ggactttttg gttcgcagca tgtcagcgtc gacagcagca ccacacaaac cgtctttatg
    25021 tacacctggc ttagcatggc gtttatcatc gcccttccgg cgttcatctt tgccagagtg
    25081 ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa aaaggagcgc gcaatgaagt
    25141 tctggcgacc tggtattacc ggcaaactgt ttctggcgat tttcgccacc tgcattgtct
    25201 tgctgatcag tatgcactgg gcggtgcgta tcagttttga gcgtggcttt attgattaca
    25261 tcaagcatgg taatgaacag cgattacaac tgttaagtga tgcgcttggc gagcagtatg
    25321 cgcagcatgg caactggcgc ttcctgcgca acaatgatcg ctttgtcttt cagatcctgc
    25381 gttcatttga acacgataat tcggaagata aacccggccc gggtatgcca ccacacggct
    25441 ggcgtactca gttctgggtg gttgatcaaa acaacaaggt gctggttggt ccgcgagcgc
    25501 cgattccacc tgacggtaca cggcgaccca ttctggtcaa cggtgcggaa gtgggcgcgg
    25561 tgattgcctc ccctgttgag cggctgaccc gcaatactga tatcaatttc gataaacaac
    25621 agcggcaaac cagctggctg attgtcgccc tggcaacgtt actcgcggca ctcgctactt
    25681 ttctgctggc gcgcggtttg ctggcaccgg taaaacgact tgtcgatggc acgcacaaac
    25741 tggcggcggg cgatttcact acccgcgtaa cgcccaccag tgaagatgaa ctgggcaaac
    25801 tggcgcaaga cttcaaccag ctcgccagca cgctggagaa aaaccagcaa atgcggcgcg
    25861 attttatggc cgatatttct cacgaactgc gtacgccatt agcggtgctg cgcggtgaac
    25921 tggaagccat tcaggatggc gtgcgcaaat tcacgccgga gacggtggcg tctttacagg
    25981 cggaggtcgg tacactgacc aaactggttg acgatcttca tcagttgtcg atgtctgatg
    26041 aaggcgctct cgcctatcaa aaagcaccgg tagatttgat cccactgctg gaagtggcag
    26101 gcggcgcatt tcgcgaacga tttgccagcc gtggcctgaa actgcaattt tccctgccag
    26161 acagtatcac cgtatttggc gatcgcgacc gtttaatgca gttattcaat aacttactgg
    26221 aaaacagcct gcgctacact gacagcggcg gcagcctgaa aatctctgcc gagcagcacg
    26281 acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg tgtcagtgac gatcagctac
    26341 aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg caaccgcgcc agcggcggtt
    26401 ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc acataatggc cgcattattg
    26461 ccgcccattc gccttttggc ggggtaagca ttacagtaga gttaccgctg gaacgggatt
    26521 tacagagaga agtatgaccg agttaccaat cgacgaaaac acaccgcgta ttttgatcgt
    26581 ggaagatgaa ccgaagctgg gacagttgct cattgattat ctgcgtgctg cgagctatgc
    26641 gccgacgctt atcagccacg gcgatcaggt actgtcgtac gtgcgccaga cgccgccgga
    26701 tctgatcctg ttggatctga tgctccctgg caccgatggc ctgacgctgt gccgggaaat
    26761 tcgtcgtttt tctgacattc ctatcgtgat ggtgacagca aaaatcgaag agatcgaccg
    26821 cctgctaggg ctggagattg gcgcagatga ctacatttgc aagccgtaca gcccacggga
    26881 agtggtagcg cgtgtcaaaa ccattttgcg ccgttgcaaa ccgcagcgtg agttgcagca
    26941 acaggatgct gaaagcccgt tgattatcga cgaaggtcgt tttcaggctt catggcgcgg
    27001 taaaatgctt gacctgacgc ctgcggaatt tcgtctgctg aaaacgctct ctcatgaacc
    27061 aggaaaagtg ttctcccgcg agcaattgct caatcatctt tatgacgact accgcgtagt
    27121 aaccgaccgt accatcgaca gccacattaa aaacctgcgc cgcaagctgg aatctctcga
    27181 cgccgaacag tcatttatcc gcgccgttta cggcgtcggt taccgctggg aagccgacgc
    27241 ctgccgcatc gtttagtttt agcgacatta ctttgttagc cggagatggc attcagacca
    27301 gatccggcaa cattatccca cgcatggtca gcaaactgac attatctctc cgccctggca
    27361 ggcatcagaa aatcgctcat actttaatcg gtaaacagca cctttagatg ctgttttcga
    27421 tacgcaattt caatcaagga gtcattatgg ctggttggtt tgaactcagt aagagcagtg
    27481 atagtcagtt ccggtttgtg ctaaaagcgg gcaatggtga gacgatcctc accagcgagc
    27541 tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc ggtgcgtagc aacagcccgc
    27601 aagaagaacg ctatgagaaa aaaacggcaa gcaacggcaa attctatttc aatctgaaag
    27661 ccgccaatca tcaaattatc ggctccagcc agatgtatgc caccgcgcaa tctcgtgaaa
    27721 ccggaattgc ctccgttaaa gccaatggca caagccagac ggtgaaagac aatacgtaat
    27781 tacgatgccg ggcgtgggtt gttggcgtcc ggcgctttac cagatccccc ctacagcgct
    27841 acaatgcccg cccttaaagt gggggcactc ccctaaccgc ttcatcaggt gaagcggatc
    27901 tgacctgtca tcagaacgag agaattatgt ttaaaccgga actcctttcc ccggcgggaa
    27961 cgctgaaaaa tatgcgttac gctttcgctt atggcgcaga tgctgtttat gcgggccagc
    28021 cgcgttactc cctgcgtgtg cgcaacaacg aattcaacca cgaaaacctt cagctcggca
    28081 tcaatgaagc ccacgcgctg gggaaaaagt tttatgtcgt ggtcaacatt gcaccgcaca
    28141 acgccaagct gaaaaccttt atccgtgacc tgaaaccggt ggtggaaatg gggccggatg
    28201 cgctgattat gtccgatcca gggctgatta tgctggtgcg tgagcacttc cctgaaatgc
    28261 cgatccatct ctcggtacag gctaacgccg tgaactgggc gacggtgaaa ttctggcagc
    28321 aaatgggact gacccgcgtg atcctctctc gcgagctgtc gctggaagag attgaagaga
    28381 tccgcaatca ggtgccggat atggagatcg agatcttcgt tcacggcgca ctgtgcatgg
    28441 cctactccgg tcgctgcctg ctctctggct atatcaacaa gcgcgatccg aaccagggta
    28501 cctgcaccaa cgcctgccgc tgggagtaca acgtccagga agggaaagaa gatgacgttg
    28561 gcaacatcgt acacaagtac gagccgattc cggtgcaaaa tgttgagccg acgctgggta
    28621 tcggcgcgcc aaccgacaaa gtgtttatga tcgaagaagc ccagcgtccg ggcgagtata
    28681 tgaccgcgtt tgaagatgag cacggcactt acatcatgaa ctcgaaagat ctgcgcgcca
    28741 tcgcccatgt agaacgcctg accaaaatgg gcgtgcattc gctgaaaatc gaaggccgta
    28801 ctaaatcttt ctactattgc gcacgcaccg cacaggttta tcgtaaagct atcgatgatg
    28861 ccgctgcggg caaaccgttc gataccagcc tgctggaaac tctggaaggt ctggcgcatc
    28921 gtggctatac cgaaggtttc ctgcgtcgtc atactcacga cgattatcag aactacgaat
    28981 acggttattc agtttctgac cgccagcagt ttgttggtga gtttaccggt gagcgcaagg
    29041 gggacctcgc ggcggtagcg gtgaaaaata aattctccgt tggcgacagc cttgagctga
    29101 tgacgccgca aggcaacatt aactttaccc ttgagcacat ggaaaacgcc aaaggcgaag
    29161 ctatgccggt agcaccaggc gatggttata ctgtgtggct cccggtgccg caggatcttg
    29221 agctaaatta cgcgctgctg atgcgtaatt tctccgggga aactacgcgt aacccccacg
    29281 gtaagtgatt aatttcgatt atttttcccg gatggaaaat tcttagaaac cgatcacata
    29341 cagctgcatt tattaaggtt atcatccgtt tcgctgaaaa acataaccca taaaatgcta
    29401 gctgtaccag gaaccacctc cttagcctgt gtaatctccc ttacacgggc ttatttttta
    29461 cgcgtaatac aatgaaataa aaggatttat ttctggtcac gtccacatat tgaccacatc
    29521 gacaaaaaaa gcccctcaac tgaggggctt tctgtttgta attacatcca cataatttgc
    29581 tgccctgacg gcaacgggtg cggtcttacg gcgtggactt ctcccggctt cacgatgtat
    29641 cgctgtaccg actcataagt gatgaacgtg gcgctgcaat tcacgttctg acactggtga
    29701 taacgctctt ttgtcgtgtc agtgatatag cgacttgtac gcgcatgtgc ggcatgctgg
    29761 cataaaggac aatgaaacat cgcgagcacc tcttccggtt ttgttgatgg tgccatttta
    29821 gttaatttat ccttataaaa caaacagata aaataaaaac atcattcatc atcttctgtt
    29881 tcgtattcca catcagaaag cctgacctca agctctaagg acgtcgtgaa gccgctatta
    29941 ttcagaaaat gtgtcacctt agtgatggtc cagtcctgct cgtctatgac gcgcttaaag
    30001 cctgacactc tgaccggtgt ttccgtgtaa atatctgcac gaccggtagc caggatgatg
    30061 gagaactccg caacgccccg ttgcagtttg tcccacttcg cctgagcagc acgcatggcc
    30121 tgtgctttcg tggcatatac cgtggtcagg gcaaaaacgt tgtcagcctc accggccatg
    30181 tattcacctt cgcgcgcttc cggtactttt ggcgctttct tctgcctgac cggtttcgct
    30241 ttcgggtgct ccagtgcgcg caggtgtttc tctttctttt tgcgtttcag ttttaccttc
    30301 tgcttttgcg gtttcgggtc tttggtgtgt aaccactttg ccgttacacc ggtataggct
    30361 ccacggtcag caatcgcaaa atgatggcgg tcgccgtcgc tgcgggttat ggtgacctgc
    30421 gggatttttt taccgctggc cgtcaccccc tgccccgctt tgagaaacaa cagttttccc
    30481 atttttaccg agacctcacc gccgttgcgt tcagcaaggc gggtcaggaa ttttgcatca
    30541 gactcctgcg actggtcgat gtgcgggatt ttaattccgg ccagtgacgg cgcgacactg
    30601 gcttccagcc tgttacggga ggctattgcc tcaacaatcg caccgagcgt ggtgtcatgc
    30661 caggagtctt cacggcggga attgagcgtc ccgcgaaaat ctgcactccg ggcgcggatg
    30721 gtgaccacat ccggcgcgcc ccggtgttca acctcatcaa cggtaaattt ccctttgcat
    30781 accagggcaa aacctttcca gccgatatac accgtcagga cagcgccacg aatcggcagc
    30841 ccgacctgcc cgtcggcatc gttcagttca atatcaagct ggtcagcctc aaagccccgg
    30901 ttatccgtca gagtcatgct catcagacgg tcgctgatat tgccggtaat atccctgctg
    30961 tcgagcatca gcatgtaatc cggcgtcagc gtactgcctg catcaaatgt cagtgcatcc
    31021 agcattatcc cgcccccgtc atacctgtga atttagtcgc catactgcca gccttaccga
    31081 tgagcgattc cgcctgttta ccgatatcgc cataaagcgc ggccagtggt tcgtcaacgc
    31141 gggtgagcga cagcgtaaaa tcaatttttc ggggtgtgcc gtctgcaaag aaaatactcc
    31201 ctgtttcact caccctgctg atgacataca tgccgtaaat catgccggtg ccatccagca
    31261 acggccacgc ccgaccttcc tctgccatca gcctgagcgt ggtcatcgtc agcttgccgc
    31321 cggtcagttc gggataaagc acaccggcca gcgtcatgtt ttcctcgcca acgccgagaa
    31381 actggtaggc atcccgttta ccgatacggg aatttgacgg ccagcgataa tctgattcac
    31441 gctgcatggt ctggtgtggc agcgtctggc gcataaaaac aaacatacct aacgcgagca
    31501 tcatttttcg tcacctcctt aaccgtcatg catcatgctg gcacgggcgc gcgcacgttt
    31561 atcccgctcg tatttttcga gcgcatcctg taactggcgg tcaagctgtg tccccggcgc
    31621 agtaccaccc gtcaggctga tgtgatattc gtttttactc tggtccacat aagagcggcc
    31681 agccggtgcc gtgaccggct gataagcctg atagcctgca taagagctgg tcgccggaat
    31741 ataaccaccg gtgccatacg tggcggcatg agttctggcg gcggtctggt caagtgtgtc
    31801 tgactctttg ttgataacac cgagtttttc cagtacccag tcaataccac tgcgcagttt
    31861 gttgaacgca ttaagcggca gcatcaacgc gtcagccagt gcctgcccga acatgacgcc
    31921 cgtgtcacgg caacggttca gggtgtcctg ggtggctttg accggggcaa tcagtttttt
    31981 aaaccactgc cacgcggcct gtaacttttc acccagccag tcaaacagcg gcttaagtgg
    32041 cgtgaacagt tcccccaccg gcgcaaatgc agctttcagc ccttcaacca caccgccaaa
    32101 gaatgcgctg acaggctccc agtatttacg gataagcaac gccccggcga caattgcagc
    32161 caccacggcc acaaccggcc agctaatcgc cccgatggcc gtcataacag cactgccaac
    32221 cgtcgtgaag attgccccca ttgcgcctgc tgccgcgatg atggcattaa tgccggtgat
    32281 aaccggccag gctacaagac caattgcacc gatgatgcca ataagcgcca gtgcgccacc
    32341 gacaataagg ccgatggttg acgccagtga tttgtttttc tggatccagc cgtcgagttt
    32401 taacacatac tttgtggccg tctgcgtgag attacgcagt gcgccttcct gttggtcaaa
    32461 caggtcagtc cccaccgcct cataagcgga ctgaaactcc ttaaagtcac cgccgaggtt
    32521 gtcctgcatg atatttacca gctctgcggt cttcccgtct gaggctttaa acgcagcggt
    32581 cagtttgtcc agctttccgg ttgaggcggc agtcatcagc acggcggcgg ctgaactggc
    32641 ctcctccccg aaaatggttt tcatgtattc agcctgctgg gcagtaccga gccggttttt
    32701 ctcaaaactg gcctgcattt ctttcagaat ggtaaatatt ggtcgggtat tccccttgct
    32761 gtctgaggtt ttcactccaa gctctttgag tgcatcccat gcttttcccg tcggtgcctg
    32821 caggcgactt aacacggcac ggcttcccgt ccccgccatt gagcctgtga tttttgcatc
    32881 atgcagcgcc ccgaccattg cggcggtttc ttcaatgctg acaccggcat tttttgccac
    32941 aggtgcggca taggtcagcg catcgctcat accgtcaaaa tcggcggcgg ttttgttcat
    33001 cgtcatggag agaacatccc cgatatgagc aaccttatcg tttgaaagct gaaaggcaga
    33061 tttcatcccc atcagcaggg cggcgttttc ttccatcgtg cgacggttcg ccagcgccat
    33121 attcagcgtg accggcgttg ccgcctgaat ggcatcaaca tccccacccg ctttcgcgat
    33181 gataatctgt gcaccggccg catcatccgc cgaggcggcg gtattgtcgc cgagctggcg
    33241 cgcctgcttg cgtagcgcgg ccatttcggc ggagtctttt gccacaccga gcacggcctg
    33301 taattctgag tttttctgcg caaattcata accgggcatc agcagcttaa caccggccat
    33361 cgttcccgcc gccgcaatcc ccacaccggc agcgcccact gaggccatat ttccggccag
    33421 ctcctttcct gcctgatagc gctgttttac tgcgttaagt tttgcctgtt gcgcactgac
    33481 acgcgccagc gcgtcacgct gccggttaag ctgtgcggtg gtttcactga tacggttttt
    33541 cagccccagc tcatcatgtg caagattgcg ggtattaatt cccacagcgg ccagttcccg
    33601 ctgctggcgt ttaacggaat ccgtcaggcg gttatatttc gcctgtaagt cctccgccgc
    33661 acgctttgcg gattccagca ctttcgcctg agcacgggtc ggacgttcgg tgtttttaaa
    33721 ctgtgtggca agggcttcgg cctcctgccg tgccttttca agtgcatgac cagtcacggc
    33781 gagctgtgcg ctggtcttgc ggaatccctc aatacgggat gcctgcccgt tcagctcgcg
    33841 cagtgatttt tgtgtttccc ggatatcccc cgacagcgac ttactcgctg tgcggatgga
    33901 tttaaacggg cgggatgcct ggtcaacagc cctgagcaat acctgtaatt ttacattgtt
    33961 actcattcgt gtttccgctt cgcctgagcg ccttttcgcg ccatgtgatg agttcggtca
    34021 ggctcatggg atacagttct gatggcggcc agtgaaatac cactgccaca tccgccatca
    34081 ggtcatcgac cgagagattt ttcggaaacg tcactgcacc gagttcggcg acaaaaaacc
    34141 gaccacctta ccggccagcg ccacaaggtc aggcagttcc agcgcggcga cctcctgttc
    34201 ggtcagcatc ggtgccgtca tgcgcggcag cactttaatc agtgcatcga cttcggagtt
    34261 cgcgaccgca gccagactga caccgcgcag cgtcccggca ttgggtttca tcagcgtgac
    34321 ctgttcgata acctgctcac cacgcttgac cggattgtcc agggtaatca cattttcttt
    34381 gttcatggtt ttctcacttc tgaatcaggg ttaaccggtc agcctggctg accggatgaa
    34441 aatcacaggc cgatattgcg gcggtgttgc tccagccggt cgacgccgtt caccttctca
    34501 atcatgttga tggtgtcgat ttcgaccagc tccttaccgt ccatcgtcag ccggaaatag
    34561 gtgcagacca cggagatttt cgactcggtg tcttctccct gtttaccctc gccggtgtcg
    34621 atttctttct gacggccacg catgaccacc tcgacggcca ccgtttcgcc ggtatcgtcg
    34681 cgctggtaag agcctgcaaa acgaatcggt acggcatcca caccggttgc ggcgtaaagc
    34741 tcccagataa ccgaatccgg gaagccaccg agcgaccact ccattgacag cgcatcgtca
    34801 tcaaggccga ggtctaccgg tgcgctgcca ttcatccccg caccgcgata gttttcgagc
    34861 ttacgggtca gttttggcag cgtgacggac ttcgcgacgc cctgatagct gtagccgttc
    34921 agaaagacgt tcattaactt gagtttgcgc ggcattgcca tcggtcaggc tccttaattg
    34981 ctgttaaccg aggtgaccag atttgccagg tatttatcgg tgatacgctg gcgcagggtc
    35041 aggttttcaa gaggaggtac cggtgtatag tcgtagtcga tatacagttt tccggccttg
    35101 agggtttccg catcgctgga ttcttcgctg aaccagcagg tcgcatccac gatatagccg
    35161 tttgttttca gctcacggaa tttggcattg atgccgtcaa cgatgtcgcg aatcagcgtt
    35221 gcggtgatgg gcttgtccac cgcccacatg tgcgcctcag ccatcgtgtc ggccagcacc
    35281 tgcgcggtgc gggtgtagtt ttcaaagagg aacagcgggt catcagtgca ggtacggtta
    35341 ccccagaagc ggaaaccgtc gcggcgaatc agcgtagtga cgcctgactc gttaagcagg
    35401 tcagcatcgg tgccggactc ctgcaaatcc cagaatacag atgcgctgat gccgttaaca
    35461 ccgtttaccc cgacgttgga cagcgtttta tgccagccct gctcctggtc gattttagcg
    35521 cgcagaccca gcgcacgggc ggtggcatac gcggtggcgg tggtgctgct gaccgtatcc
    35581 catgcgagga aatccggcca gatgaccatc agctcacgct ggctgaaatt ctggcggtag
    35641 gctttcacct cggaaatggt tttacagccc catgcgctga tatacccgaa agcgcgcagc
    35701 ttctgacaga ctgatgccag tgcaacagcc acctctttgg tgtccagtcc cggcacgccg
    35761 agaatacgcg gtttaacacc ggttaccgac tctgccgcca gcagggcttt cagtccggta
    35821 tactgaccgt tttcgtcagt ggtgccgatg atattggaaa cggtctgcgc gagtttcgtt
    35881 tcttcgtcgt cgccggtgcc gtcttcaaca cgcacgacaa cggtaaccgg ttttgactgg
    35941 tcagcgatgg cctgcaacga tgccgccagc gtgccttttt taccggcctt tgcaattgcg
    36001 ctctgcacat tggtaatcag cacaggttta ttgaggggga aggtttccgc atccgcatcg
    36061 ctggccgtgc agaccatgcc gacaatggcg gtggatacgg tggaaatgac gcgggtgccg
    36121 tcgttaatct ccagcacctg cacgccgtga tgataatcac tcatccgttt aactccgtgg
    36181 ttaatgggtg agtgatattt tcagttgtgc cggagatgtc aggctatttg tcccggttgg
    36241 ctaagggatg acacaattta ttctttgtcg ctgatgaggg gaatttttta tagagcgtgg
    36301 acaggccaat atcaaaaatc agcgccacac gttgacgaga ttcccccgca gccagcaacc
    36361 gtccgacctg ctcccactca ctcgtggtga gtttcggtcg tctgccacca atacgacctt
    36421 tggctctggc cgcttccagt ccggcgcgtg tccgctcgac aatgagttct cgctccattt
    36481 ctgccagggc ccccatcaca tgaaagaaaa agcgccccat cggcgtgctg gtatcaatag
    36541 catccgtcag gctgcgaaaa ttaacgccgc gttcgcgcag ttcctcaacc agaatgacca
    36601 gatgccgcat actacgcccc agcctgtcca gcttccagac taccagagtg tcacctgctg
    36661 ataatgttct gagcagtttt ttcagtcccg gtcggtcgga cttcgtgccg ctgattttat
    36721 cctcaaaaat cagctcacat cctgcacact ccagcgcatt acgctgcaat tccgtgttct
    36781 ggtcatttgt tgacacgcgt acatagccaa taagcatgag catctccctg aataaaaacc
    36841 ggagatgatg ccagttaccc gttatttctg cattttcata aac
//
LOCUS       DAZKFZ010000024        36194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000024 DAZKFZ010000000
VERSION     DAZKFZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            215..1588
                     /gene="aroP"
                     /locus_tag="ACHOQW_001138"
     CDS             215..1588
                     /gene="aroP"
                     /locus_tag="ACHOQW_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP9572245.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1631..2485)
                     /gene="ampE"
                     /locus_tag="ACHOQW_001139"
     CDS             complement(1631..2485)
                     /gene="ampE"
                     /locus_tag="ACHOQW_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP9572246.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2482..3033)
                     /gene="ampD"
                     /locus_tag="ACHOQW_001140"
     CDS             complement(2482..3033)
                     /gene="ampD"
                     /locus_tag="ACHOQW_001140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP9572247.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3121..4014
                     /gene="nadC"
                     /locus_tag="ACHOQW_001141"
     CDS             3121..4014
                     /gene="nadC"
                     /locus_tag="ACHOQW_001141"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP9572248.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4217..4657
                     /gene="ppdD"
                     /locus_tag="ACHOQW_001142"
     CDS             4217..4657
                     /gene="ppdD"
                     /locus_tag="ACHOQW_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP9572249.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4667..6052
                     /gene="gspE"
                     /locus_tag="ACHOQW_001143"
     CDS             4667..6052
                     /gene="gspE"
                     /locus_tag="ACHOQW_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP9572250.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6042..7244
                     /gene="hofC"
                     /locus_tag="ACHOQW_001144"
     CDS             6042..7244
                     /gene="hofC"
                     /locus_tag="ACHOQW_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP9572251.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7279..8322)
                     /gene="guaC"
                     /locus_tag="ACHOQW_001145"
     CDS             complement(7279..8322)
                     /gene="guaC"
                     /locus_tag="ACHOQW_001145"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9572252.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8478..8522)
                     /locus_tag="ACHOQW_001146"
     CDS             complement(8478..8522)
                     /locus_tag="ACHOQW_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572253.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8547..9167
                     /gene="coaE"
                     /locus_tag="ACHOQW_001147"
     CDS             8547..9167
                     /gene="coaE"
                     /locus_tag="ACHOQW_001147"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9572254.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9167..9910
                     /gene="zapD"
                     /locus_tag="ACHOQW_001148"
     CDS             9167..9910
                     /gene="zapD"
                     /locus_tag="ACHOQW_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP9572255.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9920..10117
                     /gene="yacG"
                     /locus_tag="ACHOQW_001149"
     CDS             9920..10117
                     /gene="yacG"
                     /locus_tag="ACHOQW_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP9572256.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10208..10606)
                     /gene="mutT"
                     /locus_tag="ACHOQW_001150"
     CDS             complement(10208..10606)
                     /gene="mutT"
                     /locus_tag="ACHOQW_001150"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP9572257.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10666..13371)
                     /gene="secA"
                     /locus_tag="ACHOQW_001151"
     CDS             complement(10666..13371)
                     /gene="secA"
                     /locus_tag="ACHOQW_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9572258.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13433..13945)
                     /gene="secM"
                     /locus_tag="ACHOQW_001152"
     CDS             complement(13433..13945)
                     /gene="secM"
                     /locus_tag="ACHOQW_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP9572259.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14176..15093)
                     /gene="lpxC"
                     /locus_tag="ACHOQW_001153"
     CDS             complement(14176..15093)
                     /gene="lpxC"
                     /locus_tag="ACHOQW_001153"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP9572260.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15194..16345)
                     /gene="ftsZ"
                     /locus_tag="ACHOQW_001154"
     CDS             complement(15194..16345)
                     /gene="ftsZ"
                     /locus_tag="ACHOQW_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9572261.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16406..17668)
                     /gene="ftsA"
                     /locus_tag="ACHOQW_001155"
     CDS             complement(16406..17668)
                     /gene="ftsA"
                     /locus_tag="ACHOQW_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9572262.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17665..18495)
                     /gene="ftsQ"
                     /locus_tag="ACHOQW_001156"
     CDS             complement(17665..18495)
                     /gene="ftsQ"
                     /locus_tag="ACHOQW_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP9572263.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18497..19417)
                     /gene="ddlB"
                     /locus_tag="ACHOQW_001157"
     CDS             complement(18497..19417)
                     /gene="ddlB"
                     /locus_tag="ACHOQW_001157"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9572264.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19410..20885)
                     /gene="murC"
                     /locus_tag="ACHOQW_001158"
     CDS             complement(19410..20885)
                     /gene="murC"
                     /locus_tag="ACHOQW_001158"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9572265.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20939..22006)
                     /gene="murG"
                     /locus_tag="ACHOQW_001159"
     CDS             complement(20939..22006)
                     /gene="murG"
                     /locus_tag="ACHOQW_001159"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9572266.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22003..23247)
                     /gene="ftsW"
                     /locus_tag="ACHOQW_001160"
     CDS             complement(22003..23247)
                     /gene="ftsW"
                     /locus_tag="ACHOQW_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP9572267.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23247..24563)
                     /gene="murD"
                     /locus_tag="ACHOQW_001161"
     CDS             complement(23247..24563)
                     /gene="murD"
                     /locus_tag="ACHOQW_001161"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9572268.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24566..25648)
                     /gene="mraY"
                     /locus_tag="ACHOQW_001162"
     CDS             complement(24566..25648)
                     /gene="mraY"
                     /locus_tag="ACHOQW_001162"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9572269.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25642..27000)
                     /gene="murF"
                     /locus_tag="ACHOQW_001163"
     CDS             complement(25642..27000)
                     /gene="murF"
                     /locus_tag="ACHOQW_001163"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9572270.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26997..28484)
                     /gene="murE"
                     /locus_tag="ACHOQW_001164"
     CDS             complement(26997..28484)
                     /gene="murE"
                     /locus_tag="ACHOQW_001164"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP9572271.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28471..30237)
                     /gene="ftsI"
                     /locus_tag="ACHOQW_001165"
     CDS             complement(28471..30237)
                     /gene="ftsI"
                     /locus_tag="ACHOQW_001165"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP9572272.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30253..30618)
                     /gene="ftsL"
                     /locus_tag="ACHOQW_001166"
     CDS             complement(30253..30618)
                     /gene="ftsL"
                     /locus_tag="ACHOQW_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9572273.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30615..31556)
                     /gene="rsmH"
                     /locus_tag="ACHOQW_001167"
     CDS             complement(30615..31556)
                     /gene="rsmH"
                     /locus_tag="ACHOQW_001167"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9572274.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31558..32016)
                     /gene="mraZ"
                     /locus_tag="ACHOQW_001168"
     CDS             complement(31558..32016)
                     /gene="mraZ"
                     /locus_tag="ACHOQW_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9572275.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32618..33622)
                     /gene="cra"
                     /locus_tag="ACHOQW_001169"
     CDS             complement(32618..33622)
                     /gene="cra"
                     /locus_tag="ACHOQW_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP9572276.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33704..33790)
                     /locus_tag="ACHOQW_001170"
     CDS             complement(33704..33790)
                     /locus_tag="ACHOQW_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP9572277.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33802..34293)
                     /gene="ilvN"
                     /locus_tag="ACHOQW_001171"
     CDS             complement(33802..34293)
                     /gene="ilvN"
                     /locus_tag="ACHOQW_001171"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9572278.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34296..36020)
                     /gene="ilvI"
                     /locus_tag="ACHOQW_001172"
     CDS             complement(34296..36020)
                     /gene="ilvI"
                     /locus_tag="ACHOQW_001172"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP9572279.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(35962..>36194)
                     /locus_tag="ACHOQW_001173"
     CDS             complement(35962..>36194)
                     /locus_tag="ACHOQW_001173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572280.1"
                     /translation="DLLSFLSFVMEDFIVSFHLSSCLFLLPGVYVSGCQLLKQDRTVN
                     VFYTFFPSNSEAGHGDVVWSRDGRPIAYRSGR"
BASE COUNT         8673 a        10145 c         8885 g         8491 t
ORIGIN      
        1 gctttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat
       61 tcgctgccgc ataccattat tcttgatctg acggaagtct ttttgtaact attcaaactt
      121 ctttgatgta aacaaattaa tacaacaaac ggaattgcaa acttacacac gcatcactgc
      181 gtagatcaaa aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga
      241 gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat
      301 agggacaggg ttattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat
      361 cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat
      421 ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag
      481 ctttgctggc ttcgcttctg gctggaacta ctgggtactg tacgttttag ttgccatggc
      541 agagctgact gctgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt
      601 ttctgccgcc gtgttctttg tggtgattaa cgccatcaac ctgaccaacg taacagtgtt
      661 tggtgagatg gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat
      721 cttcggcggc tggctgctgt tcagtggtaa cggcggtccg caggcaagcg ttagcaacct
      781 gtgggatcag ggcggtttcc tgccgcacgg cttcaccggg ctggtgatga tgatggcgat
      841 tatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa
      901 cccggagcaa agtatcccga aagcaactaa ccaggttatc taccgcatcc tgattttcta
      961 tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac
     1021 cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa
     1081 catcgtggta ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg
     1141 tatgctgttt ggtctggcac aacagggtaa cgcgccaaaa gcgctggcgt ctgtcgataa
     1201 acgcggcgta ccggttaaca ccattctggt gtctgcgctg gttacagcat tgtgcgtatt
     1261 gattaactat cttgctccgg aatccgcatt tggcctgtta atggcactgg tggtatccgc
     1321 actggtgatc aactgggcga tgatcagtct ggcgcatatg aagttccgtc gcgccaagca
     1381 ggaacaaggc gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg
     1441 cctgctgttt atggcggtgg tactggtgat tatgctgatg accccaggaa tggcgatttc
     1501 ggtatacctg atcccggtat ggctggtggt gttaggtatc ggctatctgt ttaaagagaa
     1561 aaccgcaaaa gccgtaaaag cacattaatc tctctacgcc ctcacccgta cagggtgagg
     1621 gcaataatct ttacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca
     1681 cgaacgaggt tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat
     1741 gcggttcacg cgccagagag aactgcgcca gacgcgttaa tacctgatat tgcgaagtac
     1801 ggaaatcacc cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg
     1861 catataccac acctgcaaga cgaaccggca cccaatccag tacatgaagc actgcatcaa
     1921 tgccggactg taaacgatga tgcggcgtct ggtatcgcgc cagccagtat tgccatgcac
     1981 gcaaaaacgc ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga
     2041 acagcggtgc aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca
     2101 aatactcacg ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca
     2161 tcgtggcacg ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa
     2221 gacgaacttt acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg
     2281 gaacgttgaa caatactccc tgcaatgcgc gtaacagtaa aaaagtcacg cccatcgcaa
     2341 taatggtcat gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt
     2401 caagacgatg atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca
     2461 ccagtaaggt tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc
     2521 ccaatcaaat gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt
     2581 catgttatta gcgatatccg gatagcaatc aatcagtgcc cgcgtaaccg ccgcaagctg
     2641 ttgatactgc gcatcggtat acgccagcgt atcggtgcct tcaagctcaa tcccaataga
     2701 aaaatcattg cagcgttcgc gcccctgata ctgagagact cccgcatgcc atgcacgttt
     2761 atcgaaagga acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac
     2821 gcgcaaatgg gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa
     2881 taatgcgtcg atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac
     2941 cagcagggtg ggtgtttcgt catccgggcg gcaatcgtaa tgtggtgagg gaacgcggcg
     3001 cgcgccaacc agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa
     3061 tacccggttc agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac
     3121 atgccgcctc gccgctataa ccctgacacc cgacgtgacg agctgctgga acgcattaat
     3181 ctcgatatcc ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat
     3241 gccaacaatg acattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg
     3301 atcacccgcg agaatggcgt attttgcggc aaacgctggg ttgaagaggt gtttattcaa
     3361 ctggcaggcg acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc
     3421 aatcaaccct tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact
     3481 gcgcttaatt ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa
     3541 ttactggaag gcaccaacac gcagttgttg gatacgcgta aaaccttacc cggtctgcgt
     3601 tcagcgctga aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct
     3661 gatgccttcc tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc
     3721 gaaaaagcgt cctggctgca cccggatgcg ccagtagaag tcgaagtaga gaatctggaa
     3781 gaacttgatg aagccctgaa agcaggagcc gatatcatca tgctggataa cttcgaaaca
     3841 gaacagatgc gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc
     3901 aacgtcactg acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc
     3961 ggtgcgctaa ctaagcacgt acaggcactc gacctttcaa tgcgttttcg ctagttgctg
     4021 acagatttat ccgctccata gtgggcggat aatctcccct tccccaacct tcttcgtaac
     4081 gcctcgcaaa tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa gcagctttcc
     4141 cgtttcgttt tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa
     4201 tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac tgatggtggt
     4261 tattggcatc attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg
     4321 caaagccgca ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt
     4381 gtgcgcgctg gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc
     4441 gcctaccacc acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac
     4501 cgggcaagaa agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa
     4561 cggcgtcacc ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc
     4621 ctgcgaagat gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca
     4681 gctcacggcc ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt
     4741 tcatgttgcg gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac
     4801 caccaaacgt attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg
     4861 cacacaacag acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct
     4921 gactcgaacg ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc
     4981 ggacaatgcc taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt
     5041 ttcaccggat gccggagtcg cattaaccgc cagattaaaa gtgctgggaa acctggatat
     5101 tgcggaacat cgcctgccgc aggacgggca attcactgtc gaactggcag gaaacgccgt
     5161 ctcatttcgt attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt
     5221 acagcaggtg agccaggcac tggatgttaa cacgctggga atgcagccgt tacaactggc
     5281 gggctttgct catgccttgc aacaaccaca gggactggtg ctggtaactg gccctaccgg
     5341 cagcggcaaa acggtcacgc tttatagtgc cctgcaaaag ctgaataccg ctgacattaa
     5401 tatttgtagc gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat
     5461 ccatccgcgt gccggactca cctttcaggg cgttttgcgt gcgttattgc gccaggatcc
     5521 tgacgtcatc atgatcggag agatccgcga tggcgaaacg gcagaaattg ccattaaagc
     5581 cgcgcaaacc ggtcacctgg tgttgtctac cctacacact aattccacct gcgaaacgct
     5641 ggtacgttta cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt
     5701 aatagcccag cgtctggtac gcaaactttg cccacattgt cgccggcagc aaggggagcc
     5761 catccacatt ccagtcaatg tatggccgtc gccgctgccc cactggcaag cacccggttg
     5821 tgtacattgc taccacggtt tttatggtcg cactgcctta tttgaagttc tgcccataac
     5881 accggtcata cgtcagctta tttccgctaa taccgacgtt gaatcgctgg aaacgcacgc
     5941 ccgacaggcg ggtatgcgaa cgctttttga aaacggctgc ctggccgtgg agcaaggctt
     6001 aaccaccttt gaagagttaa tccgcgtatt ggggatgccg catggcgagt aagcaactct
     6061 ggcgctggca tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga
     6121 gccgtacttt actgcttatg gcactacagc aacagatggt taccccacta agcctgaagc
     6181 gaattgctat caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac
     6241 tggcgacgct acttaaagcc gggttaacgc tttctgaagg gctggcactg ctggcggaac
     6301 agcatcccag taagcaatgg caagcgttgc tgcaatctct ggcacacgat ctcgaacagg
     6361 gcattgcctt ttccaatgcc ttattaccct ggtcagaggt atttccgccg ctctatcagg
     6421 cgatgatccg cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc
     6481 gtcagcaaag agcccagcgt cagttgaccg acaaagtaaa atcagcgtta cgttatccca
     6541 tcatcatttt agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg
     6601 agtttgccgc tatctataag accttcaaca ccccactacc ggcactaacg caggggatca
     6661 tgacgctggc agactttagt ggcgaatggg gctggctgct ggtgttgttc ggcattatgc
     6721 tgacgatagc caataagttg ctgatgcgcc gcccgacctg gcttatcgcg cggcagaaat
     6781 tgctgttacg catcccgatt atggggtcac tgatgcgggg acaaaaactc acgcagatct
     6841 ttacgattct ggcgctgact caaagtgcag gcattacttt tttacagggc gtagagagcg
     6901 tcagagaaac aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata
     6961 tcagtaacgg tcatcccatc tggctggcgt taaaaaatgc cggtgagttt agcccgctct
     7021 gtttgcaatt agtgagaacg ggagaggcct ccggctcgct ggatctcatg ttagacaaca
     7081 tcgcccatca tcatcgggat aacacaatgg cgctggcgga taacctagca gccttactgg
     7141 aaccggcgtt gctgatcata acgggaggaa ttatcggtac gctggtggtg gcgatgtatc
     7201 tgccaatttt ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc
     7261 cacaccagcg ttggaagatt acaggttgtt gaagatgcgg ttttcttgtt cctgcacacg
     7321 gataaatgtg gtgcgcttgg tcagttcttt caggcgtgaa gccccaacgt atgtacaagc
     7381 tgaacgcagg ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag
     7441 cttaacggtt ttaccttctg ctgcgcgata ttcagcaacg ccgccaacgt gacgtttcat
     7501 cgcagactcg gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat
     7561 gcgaccgccg ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc
     7621 gccaccaaag gctttcgcca catcgcccgg cgtggtgcag ccaccatcgc tgatgatcat
     7681 tccgcccaga ccgtgcgcag catcggcaca ttcgattacc gcagaaagtt gcggataacc
     7741 gacgcctgtt ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat
     7801 atcggcacct gagaggataa gctcctcaca catttcacca gtcactacgt taccagcaca
     7861 aatggttttg gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga
     7921 ataaccattc gccacgtcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg
     7981 tttagttttt tcgaaatccg catcagacgt accggtagaa accatcacat gtttcagcac
     8041 atcagcggaa gaattgttga taaacgcttg ccactcttcg acagaatagt gtttatgcac
     8101 agcagtcaaa atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc
     8161 catatttgcg gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa
     8221 ttgacgttcc agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag
     8281 aacgtcttta aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt
     8341 aatggctata agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg
     8401 gcgcaagact gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat
     8461 agtgagcgaa cgtttcatca atttcgcttt cgtgcgctca tttttaacgt tttgataatc
     8521 atgatgaatt cccgggaata taaattatga ggtatatagt tgccttaacg ggaggcattg
     8581 gcagtggcaa gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg
     8641 ccgatattat tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg
     8701 atcactttgg cgctaatatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg
     8761 agcggatctt cgccaacccg gaagagaaaa actggcttaa cgccctgctg catccgctga
     8821 ttcagcaaga gacgcaacac cagatccagc aagctacttc cccctatgta ctgtgggttg
     8881 tgccattgct ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg
     8941 tcagcccaga aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgagcatg
     9001 tcgaacaaat ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg
     9061 tcattgataa taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac
     9121 actatttgca gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt
     9181 cctttttgaa catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat
     9241 tcagcaactc accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg
     9301 taatgttagt gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa
     9361 agaacttgac cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca
     9421 gagccgtatt gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc
     9481 gccgcgtatc gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact
     9541 gagcatccca ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct
     9601 accccaggcg cagcgcgact gccaggtaga aacctggatt gccagcctga acccgctcac
     9661 ccaggcactt accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac
     9721 cagcctgaat ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct
     9781 gtcgctcgat tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg
     9841 ttttatgccg ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc
     9901 ctgttgctaa ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa
     9961 aacggtggtg tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct
    10021 gatcgacctc ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc
    10081 cgaaagcgat gactggagcg aagagccaaa gcagtgacat ttgtctgatg ccgcacgtag
    10141 gcctgataag acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt
    10201 gaacgcctta tccgacatac acacgcttaa gcttcgcaat taccggttca ttggctggcg
    10261 gaaaatcatc ggcattaaga ccgactagcg acatccactt accgggttgc ccttctttac
    10321 cccacggctc cccttcccag ctttcgacca gccagaacca cagagttata tgcctgtccg
    10381 ggaattcata ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacttctt
    10441 cctgaagttc acgcaccacc gcctgttccg gcgtttcacc catttcaatt ttaccgccgg
    10501 gaaactccag tttattcgcc atgtgcgcat ctgctgcgcg acgcgttata aagatttcat
    10561 tgttctcgtt gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat
    10621 aaaaaaggcg cagaatcctg cgccttttac ttcaacagtt agcttttatt gcaggcggcc
    10681 atggcactgc ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt
    10741 gcgctcaccg gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct
    10801 aagctgctgc atttgcgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc
    10861 aacctcttca ggcatacgta cctgaacttt gctcagcgta ctgataactt catatttcaa
    10921 cgactccagc atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc
    10981 tttctgtgca tagccacgca ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg
    11041 ctctttccac agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat
    11101 ctcagcacca accacttctt ctttacgctg atacacttcg atggactgcg ccagaatgcg
    11161 ctcacgcagc gtctcttcat gcagttctgg ttctttatcc agccactcag caattggtaa
    11221 atcgaggtcg aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc
    11281 cagcgactgt ggcggaatgt aggcatcaat ggtcgctttg aacacatctt cacgaatgct
    11341 gttaatggtt tcgctcacat cgctgacatc cagcaattcg ttacgctggg agtaaatggc
    11401 gcgacgctga tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt
    11461 acgactttca actttacgct gggcgttggc aatcgcttta gtcacccacg ggtgttcaat
    11521 ggcttcgcct ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa
    11581 aatacgcatc agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg
    11641 acgaccagaa cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc
    11701 gatgatatgc aggccacctg ctgccagtac cgcatcgtga cgtacctgcc agtcggcttt
    11761 aattttttca atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct
    11821 accaccgagc acaatatctg taccacgacc cgccatgttg gtcgcgatag tcaccgcagc
    11881 cggataacct gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac
    11941 attgtgctta ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga
    12001 gatagtaccc accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat
    12061 cgcctgaatt ttttccgctt cagtcatgta gaccaggtcc ggcagatctt tacgaatcat
    12121 tggacggttg gtcggaacaa cgacggtatc cagtttgtag atggagctaa attcgaaagc
    12181 ttcggtatca gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg
    12241 gaaggtgatc gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc
    12301 cacagcctgg tgcagaccat cggaccagcg acggccctgc atggtacgac cggtgtgttc
    12361 gtcaacgatg ataacttcac catctttaac gatgtagtcg acgtcacggg taaacagcgc
    12421 atgagcgcgc agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag
    12481 agactcccct tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc
    12541 acgttcggtc aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg
    12601 gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg
    12661 tttatacatt tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc
    12721 atcgatcagg atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg
    12781 ttcttcaggg ctgaacgcca tattgtcgcg caggtagtca aagccgtatt cgttgttcgt
    12841 accgtaagtg atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag
    12901 gttgataccg acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg
    12961 ttgcgccagg tagtcgttga cggtaactac atgcacgcct ttaccggtta gtgcgttcag
    13021 ataagcaggc agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca
    13081 gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac
    13141 gcgcttactt gcctcacgta ccacggcgaa agcttccggg atcagatttt ccagcacttc
    13201 gcctttttcc agacgtgcac gaaactctgc ggttttccct ttcagttctt cgtcggagag
    13261 tttttccatc tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag
    13321 ggtgcgatcg ttacgactac cgaaaacttt agttaataat ttgattagca taataaaatc
    13381 tcaaacgccc cgcgttgcgg agttaataaa atgaagtaaa ggtttattgt tgttagctga
    13441 ggcgttgagg gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt
    13501 ttgcttgtgg ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt
    13561 cctgggtcag cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca
    13621 aagattcttc agcaacgggc agcgtttgcg gtgccattgc gaaggaaaga tgacggatta
    13681 ccgtgcgaat ggcatgttga tgccagtaat ctacggaata gttcgaattc gggcggcgcg
    13741 tgttcgcttc cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc
    13801 gggttgtcgc ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta
    13861 aactcgccgc aaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc
    13921 gccagcgcgt cagtattcca ctcacgttat tgccatcccg atcgtattca aacgtattta
    13981 gcgcacaaat cttatcatta aagcctgaag tccacagcac gaatgaagat aagttgtccg
    14041 taaaagtgct taagaaaaca gcgttcgcac cgttttttat agctcttata aaattcaaaa
    14101 aaaaacgact ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg
    14161 acagtataaa tgtcgttatg ccagtacggc tgaaggcgct ttgaaggcca acggcagttc
    14221 tgcgtcgtcc tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag
    14281 cagtttgtta ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg
    14341 accacacatg aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc
    14401 aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa
    14461 gctgccgccc aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa
    14521 cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg
    14581 ctggttgctg gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc
    14641 attgtacggc ttaaattcag cccacttatc gccatcttcg acacgaacag tctctttgat
    14701 gcgaacaaat tttttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac
    14761 aaacggagcg gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat
    14821 gttatcgatg cccaagcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac
    14881 atcatgctcg ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc
    14941 cgggaaatct accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg
    15001 cgcagggcgt aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg
    15061 aacgatacgt ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc
    15121 aaccgaagtg tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa
    15181 ttccagtcaa ttcttaatca gcttgcttac gcaggaatgc tgggatatcc agataatccg
    15241 gctctttcgc agtttgcggc gcattgtcat tcacgacttt agcaaccggc ttctgctcct
    15301 gggtcagcgg agccatccca tgctgctggt agcgatccat cactggctgc tgaacctgct
    15361 tattggtcac cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg
    15421 ttacgcgcag ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt
    15481 tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt
    15541 cgaagcccgc tgtgatgtta accagcacgc cgcgtgcgcc agacaggtcg atatcttcca
    15601 gcagcggaga agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca
    15661 cgccagaacc catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt
    15721 ccacgttcat caaacccgga cgagtaatca gttcggcgat gccttgcaca gcgcctttga
    15781 gtacatcgtt cgctgcgcca aacgcatcca gcagggagat accgcggccc agaactttca
    15841 gcagtttgtc gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc
    15901 cctgctccgc gaatgccata cgcttcttgc cttcaaagtt gaagggctta gtgacgacag
    15961 caacggtcag gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac
    16021 cggtaccacc acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac
    16081 gcagtgcatc gcgatcctca tctgccgcat tgcggccaac ttctggatta gcgccagcgc
    16141 ccagtccttt ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca
    16201 gcgcttgtgc atcggtattt accgcgaaga attcaacacc ttcaatgcgc tcgcgcacca
    16261 tgtgttcaac agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat
    16321 tggtaagttc cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc
    16381 gtaatcatcg tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct
    16441 tgatccacga gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat
    16501 gtgactcttt cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat
    16561 aatccgttaa accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc
    16621 gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg
    16681 ccgccaggtg atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt
    16741 cgttgaccag gttgagcagc tcggtatagc gcggctcgat cacctctgcc agggtctgac
    16801 gttgcagact ccgcggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc
    16861 caacgatgga acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg
    16921 gcgtgccaaa ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct
    16981 tagtgtggcg caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat
    17041 cgacgacgca gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca
    17101 gaccggcaaa tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt
    17161 ttttcgccat atcgttgtgg catgtgatca ggtgcacttt tgcctgcatc cgcacgcccg
    17221 aaagccctac cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca
    17281 catgcagcac acgatgctca tcgcgtacac gcaccgattt cgcggtatgg acgacgtttt
    17341 ccacatcttc ttgcgttact tcttcttcag aaataggcac cataccaatt tcattctggc
    17401 agctgatgtg cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca
    17461 attctgcctg gtcaatggca cgttgtacgc acttgaccac ggattcgagg tcgttcaccc
    17521 cgcctttatc cataccacgc gacgggcagc tgcccacgcc aatgatattg accataccgt
    17581 cgggcagaac ttcccctact aaagcggcaa ccttcgcggt accaatctcc agtcctacta
    17641 ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg
    17701 agtagattcc tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa
    17761 atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc tgctgtagaa ccggataaag
    17821 ttctacaaag cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc
    17881 gttattcagc gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa
    17941 tctgtccttt gccagcatct gccccatttc gcgatagccc tgcaacactt cattggcgct
    18001 gccttccggg ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct
    18061 gaaggtattt ccttccgcgt ctaccatgtg ttgatcattc caccgcgcaa tcggcacata
    18121 ttcaaccaga tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat
    18181 ccacggcaga cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt
    18241 acccggctca cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg
    18301 ttcaccggtc aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagcccaa
    18361 cacgacccag ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc
    18421 cagacgcgtt ccattattgc ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag
    18481 agcagcctgc gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc
    18541 tcatacctgc ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg
    18601 tattggcttc cagcagataa aactgtccat cgctgtccag cataacgtca atacgtcccc
    18661 atcctttgca acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt
    18721 gtgacgcttc cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct
    18781 cataatcata gaaggttccg gacggttgaa tacgtactga cggtaaaatt tcttcaccga
    18841 gtatcgcaac cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt
    18901 gctgaaatgc caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc
    18961 ccacactgga accttcgcgg ctcggcttaa cgataaccgg caaacccaga gcagaaattt
    19021 ctgctaactg cttatcactc aggccttttt caaactctac gcgggttaac gctacccacg
    19081 gcgcgaccgg taaaccggca ccttgccaca gaagtttgct gcgtagttta tccattgaaa
    19141 gcgcagatgc catcactccg ctgccggtat aaggcaagcc catcagctcg agcatcccct
    19201 gcagcgtacc atcttcaccg ccgcggccgt gtagcgcgat aaacactttc tgaaagccca
    19261 tcgacttcag ttgcgtcacg tcaacttctt tcgggtcgac aggatatgcg tcaataccgc
    19321 cttcacgcag cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg
    19381 aagtcccgcc caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc
    19441 ggcttcagtt tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga
    19501 acgagaatca ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgcaggg
    19561 tccggcacca gaatgggatc tattttccca cgtccacgaa ttgtgcgaca cagcgaacgg
    19621 ctgtccgcac ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta
    19681 tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt cgcgtgtacg ggtaaaacgg
    19741 tgcggctgaa acagcattac caggttttta tccggccagc ctgcgcgcgc cgctttaatg
    19801 gtggcgtcca cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt
    19861 ttaccattca ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc
    19921 tggaagctct ccagcgcacg caaaatagcc tcgtcgtcaa tgccctcttc cgtagcaacc
    19981 gcaaccgcag ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc
    20041 atcggctctt tgtcctggcg cagcagcgta aagtgcccct gcgggccaat ctgctgataa
    20101 tcttctacac gcacgtcggc atcttcgctg aagccgtaag ttgtggtctg acgtcccact
    20161 cgcggtaaca attcgcggat caccggatca tcaacacaca tcaccgcacg accgtaaaac
    20221 ggcaggttgt gcagaaaatt aataaaagtc tgttttaaat tctcaaagtc gccctggtag
    20281 gtatccatgt ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg
    20341 aacgatgcat cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca
    20401 tgaacccccg ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct
    20461 gcgtagatgc tggagaccat cgcggtagtt gtcgttttgc cgtgcgttcc tgcaatggcg
    20521 atgccatgac gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata
    20581 cgcgcttcat gagctgcgac aatttccggg ttatcggcag aaatggcgct ggaaacaacg
    20641 accacgctgg catcacgtac gttttccggg cgatggttga aataaatcgt cgctcccaga
    20701 ttcattaact gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct
    20761 tcattggcca gaacttcggc aataccgccc ataccggcac caccgatgcc gacaaaatgt
    20821 atgtgccgaa cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta
    20881 ttcattcttt acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt
    20941 acgcccgggc agcccgactc acttcatttg ccactcgctc ggtggcatcc ggaatggatg
    21001 cagcgcgggc gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt
    21061 tggcgacagc atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt
    21121 ccagcggtag cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg
    21181 ccggtagtcc tgccgcggcg atttcactca ccgttaaagc accggagcgg caaacgacga
    21241 catccgccca cgcatacgcc gccgccatat catcaataaa ttcagtcact ttatgctgcg
    21301 gttgccccgc ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat
    21361 gccagatagt gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa
    21421 tgcgtgcgcc ctgggaacca cccactacca gcacacgaac cggaccttca cgtccagcca
    21481 aacgttgctg cggcaacggt agcgccaaca catcggtacg caccgggtta cccaccactt
    21541 ccgcattggg gaaagcacct ggaaacgcct gcatcacttt ggtggcaatc ttcgccagcc
    21601 atttattggt taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg
    21661 accacgcggc cagaccacca ggacctgata cgtagccgcc cataccgagc accacgtcag
    21721 gtttgtacgc tttcataatc gcccgcgcct gacgccaggc attgaagata cgcagcggcg
    21781 cagctatcag tgcttttata ccttttccac gcagaccaga gatacgaatg aaatcaattt
    21841 cgatgccatg ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa
    21901 cttgccaacc ctgagccatt agatggtgcg caaccgccag tcccgggaat acatgtccac
    21961 cggttccgcc tgccatcacc attaatcgct ttccttgagc actcatcgtg aacctcgtac
    22021 aaacgcctgc gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc
    22081 tgtcgacata atcagtaagc tcgaaccacc gtaactgatc agcggcaatg tcagaccttt
    22141 ggtcggtaac atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat
    22201 gccaatagaa caggcgagaa aaccggaaaa acggtggtca atttctaatg ctttacggcc
    22261 aatcgacatc gcgcgaaaag cgacgaagaa taccattaaa agtgccagca ccacaccgac
    22321 ataccccagt tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata
    22381 ctccagtttt tgtacagagt tacctaaacc ttgtccccac agttcgccgc gaccaaacgc
    22441 catcagcgat tgcgttaact gatagccgct gccaaaggga tcttcccacg ggttccagaa
    22501 tgcggtaaca cggcggatac ggtacggttc ggcgagtatc agcaacacaa ccgctgaaat
    22561 gcccataccg ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc
    22621 cagcgtagtc acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac
    22681 tgccaacacc agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc
    22741 gtcaccttta cgcaccagat agttggcgat atagcaaaac agcgacagtt ttgtcagctc
    22801 agcaggctgg atacgcagca aaccgagatc gatccaacgc gatgcccctt taaccgagct
    22861 ccccactacc agcacgatca tcagcaggat gatagatccg agcagcatcg tggcactgta
    22921 gcgttgccag aactccatcg gcagacgcag cgtaatgatc gccagaataa acgccaaaat
    22981 cagatagaca ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat
    23041 gggcattgac gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca
    23101 cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac
    23161 ccagcccttt agcgccgtgg agatccagac caggatactg aatcctggca ggcgcggcat
    23221 tttcaggcga gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca
    23281 ttgcctcgtt gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagtaga
    23341 accatatcgc ccggctgaac acgcggagcc agcaagcgca tcgcctgttc catagtttcg
    23401 gtttgttctg ccacttccgg gcgtagcgcc gccagctgca cgccgtcacg accgaaacaa
    23461 tacagacgta cgttatcgcc attcaggtaa cgcgccagtg ggctaaagtc cgccgattta
    23521 ccatcgccgc ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt cagcgccgct
    23581 tccgtactgc cgacgttggt cgctttcgaa tcgttaatcc aacgtacgcc gttatgctcc
    23641 agcacaactt caaagcgatg cggcagacca gtgaatgtgg ttaacgcttt caggctgctg
    23701 gcacgcggta accctgcggc atctgccagc gccagcgccg ccagcgcatt ggtgtagtta
    23761 tgctgcccgg aaagtttcat ttctttcacg ttcagcactt tctcgccttt cacccgcagc
    23821 caggtttcgc cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg
    23881 cagcgttcat ccgcaccgcg aatcggcatt gttaaggcat catcagcatt gacgacacaa
    23941 actttcgcat tttcgtaaat acgcaatttt gccgcacggt attgctgtaa accaaacgga
    24001 tagcgatcca tatgatcttc agtcacgttc agaatggtcg ctgctaccgc ctgtaagctg
    24061 gaggtggttt ccagctggaa gctcgacagt tccagtacgt acagttcgca ctcatcatcc
    24121 agcagcatca acgcgggcag gccaatattg ccacccacac caacgttaac ccccgccgct
    24181 ttcgccattt cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc
    24241 acaatcggtg cttgtgcttc gcgacagaac agctcgatat cgccaacgat ttcgattccg
    24301 gcatcagcgg cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc
    24361 agatctgccg ccatcagcca ttcatcattc agactgcccg tgtggcgttc tacggcttcg
    24421 ggtaatttat ccaggccagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg
    24481 cgagcgagga aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacaaca
    24541 tttttaccct ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga
    24601 ccagcatcag cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca
    24661 gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg
    24721 agccgacctg caggatgacg gaaagcgttt ctaccacgaa cacgcccccc ataatcacca
    24781 gcaggaattc ctgacgcagc agtacggcga taatgcctaa cgcaccacct aacgccagcg
    24841 aacctacatc gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagcc
    24901 ctgccccgac tatcgcggta cagacaataa ccagttcccc ggcgtgtcgc agatatggta
    24961 tgtgtaaata gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac
    25021 cggcgacaaa tacggtcggc ataattgcca gaccgtcaag accatcggtc aggtttaccg
    25081 cgttgccagt acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagttgcg
    25141 gcatcacatc tttaaagaat gggaccacca gctgcgttgc gggcgtgtct ttgccaacaa
    25201 ggtacagcgc gaaggcgaca cccagcgcaa tgaccgacat ccagaaatac ttccaacgag
    25261 cgatcaaccc tttggtgtct ttacgcacta ctttgcgata atcatcaaca aagccaataa
    25321 caccgtaacc taccagcacc accaacacgc accagacgta cggattggac gggtaagccc
    25381 acagcagtac ggagatcaca atcgccgtca ggatcataat cccgcccatg gtcggcgtac
    25441 cgcgcttgct gaagtgtgat tcaggaccgt cgttacgcac cacctgacca aaggaaagtt
    25501 tttgcaaatg agcaatcata cgcgggccca tccacaatga gatgaacagc gcggtcagca
    25561 ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga
    25621 ccaaatgttc ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc
    25681 ttccatggcg gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat
    25741 cagtgattta agacgcgtaa ttaacgcagt tttctcagca aaatgttcgc caacgccgct
    25801 ggcggtgctg atagcatggc tttgtttacc cacgcttaac acgcggtcaa taccagcagc
    25861 ttttgccgcc tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat
    25921 atcgcccacc accagcacgc ggtagcccgg catttcagcc agtacctgaa cagcggcggt
    25981 cattgaaccg acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttg
    26041 aatggggaac aaacggcctg gaacagcttt cagatttgcc agccccgctt tgatagcatc
    26101 aagcgttgcg cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg
    26161 acgccccggc aacggcagca gaacatccac gctaccggtt ggggtttgca gggtaaattc
    26221 cgtaccgtgc gaagtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattagg
    26281 tgagaaacgc cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt
    26341 gtcggcgttc ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc
    26401 tttcgcgaca cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac
    26461 cagcgcagct tccgggcgag tcagactcac agtccagaca atttcgccct gatggttcgc
    26521 gccaagttca ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac
    26581 accgatgtcg ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat
    26641 cgccgccgtc atctctttaa cggaggtttt gccggaggaa cctgtcagag caaccacgcg
    26701 cgccggaact tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt
    26761 gacgattaac tgcggcaggt cgatatccag cggacggcta accagtagtg cgcctgcgcc
    26821 gccagctttc gcctggtcgg caaaatcatg agcatcgaaa cgttcgcctt tcagggcaac
    26881 aaacaggcag cccggcgtca gttttcgggt atcagtggtt acagcatcaa gggtgatatc
    26941 tgcaccttgc agttcaccgt tgagaatgtc ggtaagttgg ctaagggtta cgctaatcat
    27001 gcaatcaccc ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg
    27061 ccaacaatct ggtaatcttc atggcctttg cccgcgacca gcaccacatc gttctcttta
    27121 gcctgcataa cggcgcaagt caccgcttca gcacggtctt ccatcacttt ggcatgtccg
    27181 gcatctaaca ttcccgccag aatatcgttg atgatggcac gcggctcttc ggtacgcgga
    27241 ttatcgtccg tcaccaccgc cacatcagca aactcttcgg caattgcgcc catcagtgga
    27301 cgcttacctt tatcgcgatc gccaccacag ccaaagacac accacagctt gcccgcacag
    27361 tgcagacgcg ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc
    27421 accaccgtcg gtttgcctgg cgcagtgaac acttccatac gtccgcaaac cggttgcaga
    27481 cgagcagcgg ttttcagcag atcagccagt ggatagccca gcgccagcag cgtcgccagc
    27541 gcgagcagca ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttcgccatcg
    27601 ccccaacttg agctaaagcg aatagtcgca ccgctgtcgt gatagttcac ttcggtcgct
    27661 ttcaaccagc gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaactgca
    27721 tccggcagtt ttgccagcca gcggcggcct acttcatcgt cggcattaat aatcgcctga
    27781 ccgcaatgat gttcagaata aagcagccat ttcgcagctt cgtagtgttc catatcacca
    27841 tgataatcaa ggtgatcgcg gcttaagtta gtaaagaccg acgccgcaaa tttcaatgcc
    27901 gccacacggt gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa agtcgcgtcc
    27961 tgatccacca gccccgccag ctcatgctga acatcaactg ccgaaccggt ggtattttct
    28021 gtcgggatca ctttccccag cagaccgtta ccaacggtgc ccattaccgc gctggtttcg
    28081 ccaagcagtt ggctccactg cgccagcagc tgggtcgtcg tggttttgcc gttggtgccc
    28141 gttacgccca cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca
    28201 gataaacgct cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt
    28261 tcaccgtcgg tcgcctcacc tttcgcctct gcaataatgg cagccacacc ttgcgctatc
    28321 gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg
    28381 cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct
    28441 ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg
    28501 attaatcaca aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt
    28561 tcgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta
    28621 tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg
    28681 cgcaacacct gcggtgtaag caatatattt attgatgtag cgaccgtccg gcccgacctt
    28741 tttcgcggta ccggttttaa tggcaatacg atagccttta atcgccgcct tcacgccgcc
    28801 gccacccggt agcgccacgc tttccatcat atgcaccaca gtgcggacaa tagattccgg
    28861 gaagacacgt tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat
    28921 gccgtagctg ccgatagttg cgtagactcg cgctaactgt aacggagtta ccattagccc
    28981 gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa
    29041 gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta
    29101 agtatctact aacgctgagg acggcatcgc taacgccagc ttggaaacac cgacgttact
    29161 cgacttctgt aatactccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc
    29221 gtggccgtta attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg
    29281 ttgcaacgcg gtcatcacca ccatcggttt aaccgttgaa cccggttcaa acacgtcggt
    29341 gatggtacgg ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga
    29401 cgggctgtta gccatcgcca gcacttcacc ggtgttgaca tccaccagca cggcgctacc
    29461 ggactcagcc ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag
    29521 gcgttcatca atactcagcg ccaggttgtg cgcagcctgg ctgtcagtag aagaaatatc
    29581 ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag
    29641 ccatttatcg aaactcttct caacgccctc aataccttga ctatcgacgt tagtaaagcc
    29701 gatgaggtga gcagtcactt cgccggatgg ataataacgg cgagattctt cacgcagatg
    29761 aattcccggc agtttcagtt ttttgatgta gtccgccatg tcagggttta cctgacgcgc
    29821 cagataaata aagcgccctt tcgggttggc gttaatgcgg gcagaaagct gatccagcgg
    29881 aatattgagc gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc
    29941 atgcacttct ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg
    30001 accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg
    30061 catgtcgccc tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc
    30121 gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat
    30181 aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg
    30241 tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat
    30301 ttgcagcttt tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc
    30361 aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg
    30421 ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat
    30481 aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac
    30541 accaggcaat gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc
    30601 tgtcactctg ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca
    30661 cgaggattct cagccacctc ttcttcgccc ggcattaatt tgcctagtgc tcgcagctga
    30721 cggccaccca gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga
    30781 ccgcggctgt tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg
    30841 atgatcgaaa gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct
    30901 atctcctcca gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga
    30961 tgtttaaact tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg
    31021 cgggtcatcg gctgttcgcg gttacgctcg acaatggctc gggcaatgcg tttggcaaaa
    31081 cgctcttcac cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc
    31141 cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc
    31201 ataaaggaaa agccgcgttc agcatcatca agttgcggtg aagagacacc aagatcgagg
    31261 agaatgccgt cgatcttgcc gataagatcg cgctcggcaa cgtattcgcc cagcgcggaa
    31321 aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc
    31381 tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga
    31441 cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg
    31501 aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta
    31561 tagagacaag tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat
    31621 atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg
    31681 tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa
    31741 caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac
    31801 acggcgctca accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca
    31861 ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg
    31921 accggcagcg ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt
    31981 gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac
    32041 tttgccccac aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc
    32101 agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta
    32161 ttttacgtcc aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaaaccctc
    32221 atcgtcatca ccgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt
    32281 tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtccct gttgcgtctg
    32341 tgtagcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgcttg ttaaatcggc
    32401 aattcatctc tgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat
    32461 tgtgaatttc agctggtgag ttaattaact ggcgggttta aaagcattta tcaataatag
    32521 tcaggaatag tcttatttac tttaaggata ttgatgtcca gtcccgtact ctacgcgcca
    32581 gagtgaaatt cacctggcgc gtatttttgt tcgcagctta gctacggctg agcacaccgc
    32641 ggcgatagag attacgttta atgcgcgtta aaccaggttt tggcttacgc ggttcgtcca
    32701 ggcttgccag gacaatctcc agcacacgct ctgcgacatc gcggtgacgt tgagccactg
    32761 ccagcaccgg gcactgtaag aagtcgagca gttcgttatc gccaaaggtg gcaattgcca
    32821 ggtcagaagg cagtttacca tcgcgacgta gcgtgacatc catcacccct tgcaacaacg
    32881 caaacgacgt tgtgaacagc gcctgcggca tcggatgcgt ttccagccat ttttcgaata
    32941 actgggcagc cgcctcccgc tcatagctgt tggcatacag gaaatgcact tcgcgcggat
    33001 catctttcca ggcagtacgg aaaccttgtt cacgcaggaa gctgacagaa agctccggca
    33061 gcgcgccaag ataaagcacc gtctcggcgg gaaacttacg taactcttcc gccagcattt
    33121 cggcatcatc ctgatcggca ccaaccacgc tggtaaagtg ttcacgatcg agggcgcggt
    33181 ccagcgcgac aatcgggaac gggtcgttag cccagcgttg ataaaaagga tgctcaggag
    33241 gcaacgacgt cgaaacaata atggcatcga cctgacgctg taaaaggtgc tcaatgcagc
    33301 gcatttcgtt gtctggctga tcttctgagc aggcaatcag cagttgataa ccccgttgcc
    33361 gcgcctggcg ttcaagatag ttagcgatgc gggtatagct ggtgttctcc agatcgggga
    33421 tcacaagacc aatagaacgt gtgcgtccag cacgaagccc agctgccacg gcgttcgggt
    33481 ggtaattgtg ctcacgcacc acagccatga ctttttcaac ggttttgtca ctcacacggt
    33541 attgcttcgc tttgccgtta ataacatagc ttgcagtggt ccgcgacact cccgccagcc
    33601 gagcgatttc atccagtttc acaattgccc cttgcgtaaa atgtaaaaac catagccatt
    33661 gtactggtat gggttaaatt cattaacatc taagcgcaga aaattaactg cggcaactgc
    33721 ttttattcca ggttcccact gatttcgcaa aaaaagccca acgtgacatg ttgggctgta
    33781 aattgcgcat tgagatcatt ctcaacgcat tattttatcg ccgcgcgaaa gtccgaccac
    33841 accagagcga gcaacttcca caattttcgc cacatcgcga atcgatgcta aaaatgcatc
    33901 aagcttatcg ctggtgcctg ctaattgaac ggtataaagc gagggtgtga catcaataat
    33961 ttgcccacgg aatatttccg tattacgttt aacttcgtca cgcccgtaac cgctggcctg
    34021 aattttcacc agcatgattt ccctctcaac atgcgcgccc tgccccaact cactcacgcg
    34081 caagacatcg accagcttgt ataattgctt ttcgatctgc tcaagcactt tttcatcgcc
    34141 cacggtctgg atggtcatac gcgataatgt cggatcgtcg gttggcgcaa cggtcaggct
    34201 ttcaatgttg tagccacgct gggaaaaaag gccaatcacg cgggataacg cgcctgattc
    34261 attttcgagt aagactgata atatccggcg cataatcagg ttctctccgt tttgcttaac
    34321 cacatttcat ccattccgcc cccgcgaatc tgcatcgggt agacgtgctc actgccatcg
    34381 acggtgacat caacaaatac caggcgatta ttacgcacct gttcaagcgc ctcgctaagt
    34441 ttgctttcca gctcttgcgg atgagagatc tggatcccga cgtgaccata ggcttccgcc
    34501 agacggacga agtctggtaa agactgcata taagattgtg aatgacgacc ggaatagatc
    34561 atgtcctgcc actgcttcac catccccaaa tagcggttat taagattaac caccagcacc
    34621 ggcagttcat attgcaacgc tgtagacagt tcctgaatgt tcatctgaat actgccgtcg
    34681 ccagtgacgc aaaccacggt ttcttctggc agcgccattt tgacgcccag tgccgcaggt
    34741 aaaccaaagc ccatagtgcc gaggccaccg gagttgatcc aacgacgggg tttgtcgaat
    34801 ggataataaa gcgcagcaaa catctggtgc tgcccgacat cggacgtaac gtaagcgccc
    34861 cccttcgtca accgccaaag agtctcgatc accgcctgcg gtttaatctt ttcactgtga
    34921 gtgtcatatt tcaggcactg acgagcgcgc cactgttcaa tctgctgcca ccagtcgcgg
    34981 atctcatcca gcggttgatg ggcggattct tgcgacaaga gttcaagcat ttgttcgagg
    35041 acctggcgag catcccccac aatcggaata tccgcagtca cggttttaga aatggaagta
    35101 ggatcaatat cgatatgcag aacagtggca tttgggcagt actttgccag attgttagtg
    35161 gtgcgatcgt caaagcggac accgacagca aaaatcacat ccgcgttatg catcgtcata
    35221 ttggcttcgt aggtaccgtg cattcccagc atgcccagtg cctgacgatg cgttgccgga
    35281 aacgctccca gccccatcaa tgaggaaaca acgggcagat ttaacgcctc caccgtttct
    35341 ttcagctgtt gatggcagcc cgccgtgatt gccccaccgc ctacgtagac aaccggtttt
    35401 tttgccgcca ccagcgtttg cagagcgcgc ttaatttgcc ctttatgtcc ggtagtagtg
    35461 ggattgtaag aacgcatact gaccgactcc ggccagacat agggtaattt gttcgccgga
    35521 ttaagaatat ctttcggtaa atcaacgacc actggtcctg ggcgaccact tgccgccagc
    35581 cagaaagcct ttttcagcac ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt
    35641 ttaaccaccg gtcgcgaaat ccccaccatg tcgcactcct gaaaggcatc gtaacctatc
    35701 aacgaggtcg ctacctgccc ggaaaggaca actaatggaa tggaatccat ataagcggtg
    35761 gcgatgccgg taatcgcatt ggtcgcccct ggacccgacg ttaccagcac gacgccgact
    35821 tccccggtcg cgcgcgccag accatcggcc atatgcactg ccgcctgctc atgacgaact
    35881 aaaacatgac cgataccacc cacggtatgc aatgcatcgt aaatatcaag gactgcgcct
    35941 ccgggataac cgaatacttg tttaacgccc tgatcgataa gcgatcggac gaccatctcg
    36001 gctccagaca acatctccat ggcctgcctc actgtttgac ggaaaaaatg tgtaaaacac
    36061 attaaccgtc cgatcttgct taagcaattg gcagccagag acataaacgc cgggtaataa
    36121 gaataaacag gaggaaaggt gaaaagaaac gataaaatcc tccattacga aggataaaaa
    36181 tgataataaa tcgg
//
LOCUS       DAZKFZ010000025        35637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000025 DAZKFZ010000000
VERSION     DAZKFZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(41..116)
                     /locus_tag="ACHOQW_001174"
     tRNA            complement(41..116)
                     /locus_tag="ACHOQW_001174"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(81..83),aa:His,seq:gtg)
     gene            complement(175..251)
                     /locus_tag="ACHOQW_001175"
     tRNA            complement(175..251)
                     /locus_tag="ACHOQW_001175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(215..217),aa:Arg,seq:ccg)
     gene            complement(354..1739)
                     /gene="yifK"
                     /locus_tag="ACHOQW_001176"
     CDS             complement(354..1739)
                     /gene="yifK"
                     /locus_tag="ACHOQW_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9572281.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1930..2670)
                     /gene="wecG"
                     /locus_tag="ACHOQW_001177"
     CDS             complement(1930..2670)
                     /gene="wecG"
                     /locus_tag="ACHOQW_001177"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP9572282.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2673..4025)
                     /gene="wzyE"
                     /locus_tag="ACHOQW_001178"
     CDS             complement(2673..4025)
                     /gene="wzyE"
                     /locus_tag="ACHOQW_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP9572283.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4022..5101)
                     /gene="rffT"
                     /locus_tag="ACHOQW_001179"
                     /gene_synonym="wecF"
     CDS             complement(4022..5101)
                     /gene="rffT"
                     /locus_tag="ACHOQW_001179"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP9572284.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5098..6348)
                     /gene="wzxE"
                     /locus_tag="ACHOQW_001180"
     CDS             complement(5098..6348)
                     /gene="wzxE"
                     /locus_tag="ACHOQW_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP9572285.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6350..7480)
                     /gene="rffA"
                     /locus_tag="ACHOQW_001181"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6350..7480)
                     /gene="rffA"
                     /locus_tag="ACHOQW_001181"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP9572286.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7485..8159)
                     /gene="rffC"
                     /locus_tag="ACHOQW_001182"
                     /gene_synonym="wecD"
     CDS             complement(7485..8159)
                     /gene="rffC"
                     /locus_tag="ACHOQW_001182"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP9572287.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8137..9018)
                     /gene="rfbA"
                     /locus_tag="ACHOQW_001183"
     CDS             complement(8137..9018)
                     /gene="rfbA"
                     /locus_tag="ACHOQW_001183"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9572288.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9037..10104)
                     /gene="rffG"
                     /locus_tag="ACHOQW_001184"
     CDS             complement(9037..10104)
                     /gene="rffG"
                     /locus_tag="ACHOQW_001184"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9572289.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(10104..11366)
                     /gene="wecC"
                     /locus_tag="ACHOQW_001185"
     CDS             complement(10104..11366)
                     /gene="wecC"
                     /locus_tag="ACHOQW_001185"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP9572290.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11363..12493)
                     /gene="wecB"
                     /locus_tag="ACHOQW_001186"
     CDS             complement(11363..12493)
                     /gene="wecB"
                     /locus_tag="ACHOQW_001186"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP9572291.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12549..13595)
                     /gene="wzzE"
                     /locus_tag="ACHOQW_001187"
     CDS             complement(12549..13595)
                     /gene="wzzE"
                     /locus_tag="ACHOQW_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP9572292.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13607..14710)
                     /gene="wecA"
                     /locus_tag="ACHOQW_001188"
     CDS             complement(13607..14710)
                     /gene="wecA"
                     /locus_tag="ACHOQW_001188"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP9572293.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14950..16209)
                     /gene="rho"
                     /locus_tag="ACHOQW_001189"
     CDS             complement(14950..16209)
                     /gene="rho"
                     /locus_tag="ACHOQW_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9572294.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16294..16395)
                     /gene="rhoL"
                     /locus_tag="ACHOQW_001190"
     CDS             complement(16294..16395)
                     /gene="rhoL"
                     /locus_tag="ACHOQW_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP9572295.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16536..16865)
                     /gene="trxA"
                     /locus_tag="ACHOQW_001191"
     CDS             complement(16536..16865)
                     /gene="trxA"
                     /locus_tag="ACHOQW_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP9572296.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16996..18261
                     /gene="rhlB"
                     /locus_tag="ACHOQW_001192"
     CDS             16996..18261
                     /gene="rhlB"
                     /locus_tag="ACHOQW_001192"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP9572297.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPVNQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18397..19881
                     /gene="gppA"
                     /locus_tag="ACHOQW_001193"
     CDS             18397..19881
                     /gene="gppA"
                     /locus_tag="ACHOQW_001193"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP9572298.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19928..21949)
                     /gene="rep"
                     /locus_tag="ACHOQW_001194"
     CDS             complement(19928..21949)
                     /gene="rep"
                     /locus_tag="ACHOQW_001194"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP9572299.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22166..22615
                     /locus_tag="ACHOQW_001195"
     CDS             22166..22615
                     /locus_tag="ACHOQW_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9572300.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22814..23095
                     /gene="ppiC"
                     /locus_tag="ACHOQW_001196"
     CDS             22814..23095
                     /gene="ppiC"
                     /locus_tag="ACHOQW_001196"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP9572301.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23141..24616)
                     /gene="ilvC"
                     /locus_tag="ACHOQW_001197"
     CDS             complement(23141..24616)
                     /gene="ilvC"
                     /locus_tag="ACHOQW_001197"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP9572302.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24766..25659
                     /gene="ilvY"
                     /locus_tag="ACHOQW_001198"
     CDS             24766..25659
                     /gene="ilvY"
                     /locus_tag="ACHOQW_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP9572303.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25711..27255)
                     /gene="ilvA"
                     /locus_tag="ACHOQW_001199"
     CDS             complement(25711..27255)
                     /gene="ilvA"
                     /locus_tag="ACHOQW_001199"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP9572304.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27258..29108)
                     /gene="ilvD"
                     /locus_tag="ACHOQW_001200"
     CDS             complement(27258..29108)
                     /gene="ilvD"
                     /locus_tag="ACHOQW_001200"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP9572305.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29173..30102)
                     /gene="ilvE"
                     /locus_tag="ACHOQW_001201"
     CDS             complement(29173..30102)
                     /gene="ilvE"
                     /locus_tag="ACHOQW_001201"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP9572306.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30122..30385)
                     /gene="ilvM"
                     /locus_tag="ACHOQW_001202"
     CDS             complement(30122..30385)
                     /gene="ilvM"
                     /locus_tag="ACHOQW_001202"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP9572307.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30382..32028)
                     /gene="ilvG"
                     /locus_tag="ACHOQW_001203"
     CDS             complement(30382..32028)
                     /gene="ilvG"
                     /locus_tag="ACHOQW_001203"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP9572308.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32031..32081)
                     /gene="ilvX"
                     /locus_tag="ACHOQW_001204"
     CDS             complement(32031..32081)
                     /gene="ilvX"
                     /locus_tag="ACHOQW_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP9572309.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(32168..32266)
                     /gene="ilvL"
                     /locus_tag="ACHOQW_001205"
     CDS             complement(32168..32266)
                     /gene="ilvL"
                     /locus_tag="ACHOQW_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP9572310.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32619..34139
                     /gene="yifB"
                     /locus_tag="ACHOQW_001206"
     CDS             32619..34139
                     /gene="yifB"
                     /locus_tag="ACHOQW_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP9572311.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34164..34502)
                     /gene="maoP"
                     /locus_tag="ACHOQW_001207"
     CDS             complement(34164..34502)
                     /gene="maoP"
                     /locus_tag="ACHOQW_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP9572312.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34621..35460
                     /gene="hdfR"
                     /locus_tag="ACHOQW_001208"
     CDS             34621..35460
                     /gene="hdfR"
                     /locus_tag="ACHOQW_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP9572313.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(35556..35631)
                     /locus_tag="ACHOQW_001209"
     tRNA            complement(35556..35631)
                     /locus_tag="ACHOQW_001209"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35596..35598),aa:Trp,seq:cca)
BASE COUNT         8699 a         9720 c         8997 g         8221 t
ORIGIN      
        1 accaattccg ccaccttcgc attgtcacaa cttctaataa tggggtggct aatgggattc
       61 gaacccacga caactggaat cacaatccag ggctctacca actgagctat agccaccact
      121 acaaatcttg ttacgcggta ttactaccac cgcagctcaa gcgccgggac taaatggcgc
      181 gcccgacagg attcgaacct gagacctctg cctccggagg gcagcgctct atccagctga
      241 gctacgggcg cttagcgccg ttgcgggggt ggataatacg gacttccccc ccctctgtct
      301 agtccctttt taaataaatt gcgcgtttgg ttacggtttg tgcgttttgc tgcttattcc
      361 tccagtttat gcgctttccc gtggcgatta aggccaaaaa ctttataaat cgccgtcacc
      421 gccagcataa agatgatgcc aacaaacagc gacatacgcg tatcttcatt aaagtacatg
      481 ccgatcaaaa cgcaaatcag gaatgccatt gttacgtagt tggcccacgg gaacaggatt
      541 gagcggaacg gatggctggc aatcgccgct ttatgtgcac gccgaaaacg cagctggctt
      601 atcaatatca caaaccatgg caccatcccc ggaagcacgc tggcactgta gacgtagaca
      661 aacacacgct gcggattggg aatgatgtag ttcaggcatg agccaattag cagaatagca
      721 atagatactg ccacacccgc aaccggtacg ccgtgacggg aaactttcgc catcgccgcc
      781 ggtaactgac ggtttttcgc cagtgcgtag agcatacgtc cgcaactgta catgccgctg
      841 ttacagccag agagcgcagc cgtcagcacc acaaagttga taatgcccgc cgctgcggta
      901 ataccgattt tggcaaaagt cagtacgaac gggctgccgt tgctgcctat ttcattccac
      961 gggaagatgg tgacgataac gaaaatcgcg ccaacgtaga aaatcaggat ccgccacagc
     1021 accttgccta cggcactgcg cagcgtcacc tgcggattct tcgcttcacc ggcagtaatg
     1081 ccaatcagct ccacgccctg gtaggacgcc accacaatac acagcgcggt caggaaccct
     1141 ttccagccac ccgcaaagaa accgccatgc tctgtgagat tgctaaaacc aatcgactgc
     1201 ccgccattgc caaagccaaa gaaaatcacg cccaggccaa tgacaatcat cacgataatc
     1261 gtggtgactt tgatcatcgc gaaccagaac tcgatttcgc cgtacaaccg caccgctgcc
     1321 agattcgcca acgccaccag cgccactgcg atcaatgcgg gtatccactg cgccatctcc
     1381 gggaaccaga actggacata aacgccaatg gcggtgattt cagagatccc caccgccatc
     1441 cacataaacc agtaagacca ggcggtgaga tagccaaaga acgggctcat ataacgatgc
     1501 gcataaacgg cgaacgaacc ggtaaccggt tcgaggaaca acatttcgcc cattgaacgc
     1561 atgatgaaaa agacgaacag cccggcgatg atataggcca acaatacgga tggcccggcc
     1621 catttcaggg tactggcggc ccccataaac aggccgacgc caatggtgcc ccccagggcg
     1681 atgagttcga tatgtcgagc ttccagccca cgctgtagct ctggtttgtt atctgccata
     1741 aatcctcgtg ttgtgtttgc atgctttccg gtgttaccgg ttatcgttat gggtacatcg
     1801 agtgttgcga atgttttcgt aattcaggag aaatggcaaa taaagcatta aaaatttgaa
     1861 tgctttgtgt aataaaaaag cagacaggcg acggagtgac cactccgtcg ctttacaaag
     1921 agaggaaaat cagaggttgc cggtgtaatg ccagcgtaaa taacgcagca aacgaagctg
     1981 acgcttaatg cggctcggct gcgaaagcag gcggtagagc cactccagcc ccagcgtttg
     2041 ccagattttc ggtgcgcgtt ttacgtgacc ggtgaaaaca tcgtaagtcc cgccaacgcc
     2101 catatacagc gcatctggat gcaccagacg gcagtcgcgc atgaagatct cctgctttgg
     2161 cgatcccatc gcaacggtga cgatttgcgc tccgctggca tgaatgcgtt caaacagcgc
     2221 ctgacgctgc tcgggtttaa aataaccatc ctgactgcca acgatattca cattccactg
     2281 gttgcgcagt ttagcttcag tttgcgccag cacttcaggt ttaccgccca caagaaatac
     2341 cgtcgtccct tctttgcctg cgcgcgccat cagctcttcc cagagatcgg caccggcaac
     2401 gcgggaaacc tgcgcctgcg ggtacttttt acgtactgaa cgtacaacgc tgatgccatc
     2461 cgcatattta aattcggcag cgttaattaa ctccctgacc tcggcgttct cttcaatggt
     2521 cagcattttt tcagcattaa tggcaaccag cgttccctgc ttaagctgcc cgtcagcaaa
     2581 cagataatcg agggcatgct gcatatcacg ccaaccaatc aactgtaagc cacgcagcgt
     2641 ataggtcggt gccgtggtgt tgttattcat tgttatcctt caacctgcgt ccggagcgat
     2701 gattttgtac gtttatgaat gagtccggcg ctttcaaaaa gccagtacaa cagttttgcg
     2761 atcatcagac atgcgccgaa gaccacgata aaaaagacca cgcgtgagac aaacgaatcc
     2821 agcccttcac gcgccagcac gatcatattg aagatcgccc caaagcagaa actgtgcaat
     2881 atcgcagcct tatagcgatt agtttcgcgg ttgcccagct catacagcca gtcgaaccat
     2941 ttgatgatca gtccaaccac aatcgccccg agcgggatga acaacgcgcc gcccatcacc
     3001 accagtgagc ctataagcgt aggcgagatc gccagtccgg agtggttatt cagcacttcc
     3061 caggtaaagt agttggctga gttcagcacc atactcgggc gacccggcca cagccaggaa
     3121 gggataaaga catagaaatc gcggacaatt ggcgccaggc cctggaagtc gatgttgtcg
     3181 tagttctgca acagcaacgc cagattctcc cacggcgaga aggtgtcgcg agtgagatag
     3241 agaaacgtat agaacgcttc atcgccgctc acattcattc catagcgttt tagcgccagc
     3301 cagaacatgc caacaatccc cagcacgccc gccgccgcca gcatccacaa cgaaatccag
     3361 ccgcgaataa tgccaataaa caggaagata gcgaatgcga tgatgatatt ggcgcgagtg
     3421 ccgccgacaa tcatataagt cagcaagcca aaggcgaccg tgctgacgag gaaaaacagc
     3481 cacgctttgc tgtcctggcg cagaaagtag accaccagca tcgccgggat gaaaaagtaa
     3541 aagaagcgtt ttaacgccac gccggagact tcactggaaa agatctgact gtaggagtta
     3601 agccggaaca gcaaaaagcc gttgtgcatg aagaagatgc cgacgcttac cagcgcgata
     3661 cccatcagga tcacccacgt aagattggtt tccacgcggt tcatggtaaa cagcggacgg
     3721 cgcggtacat cagcaacgcg tttgcgtagg cgggttttgt aggtgacgta gtaaaccgcg
     3781 tagaagcagc ccgcagaaag caacgcctgc aacaggattt ctggcggcgc gacaccaaca
     3841 tcaaagcgaa acaccagcac gctggtcagc gggaagccaa agaaaaaggt gagcagaaac
     3901 agcaatgaaa agaatacatt gaagttaaag cgcacacgac gaaactcaaa ccaggtcagc
     3961 gtggcaataa acagcgtgca gagcagccag acaacaaaca ggccactgaa ttgcagcaga
     4021 ctcatgcgac ctccccggcg gcaatcgcca acgcccgctg ccagccttgt agatagttag
     4081 ggctaaagaa ggcaatggtg tttttatcca ccgacgccaa ctggcgctgc gcttcacgca
     4141 caatatcctc gttgagatcg tcggtagtaa acagcaccgg taaatgctgt tccgtcatat
     4201 cctgccagaa cggattttcc cggttaagca cacaaggaat gcccgcctga atcagtaagc
     4261 acagcgtacc aatgccctgc tggcgggcaa aaataaagta accaagatcg cactgacgaa
     4321 gtagcgtcag ataggcgtca aattccagtt tttcactcag aacttgcaga ttttcttcgc
     4381 tgaataactc cagccccgcc tgacgaactt cctcgatgta cgcttcgtta tgaggcggat
     4441 atcccatcgg caccaccact tttaccgtat cgccaaattg ctgatgaacg gcgcgcaagg
     4501 cagcaatatg ctcattgctg cggtcgccgg agttccccac cagaatggtc attttccctt
     4561 cacgttgccg atcgttcgcc atcgtattga gagaagcatc catccgcgtc gggaagtaca
     4621 gcagttcgcc ccgcaccttt gggtgcgttt tggcaaaaaa gctcaaatca ccacgggtgg
     4681 caaatacaca gccgactcgc ttttgcgcca ggcgacgtag tgggtaaaaa agcttatatc
     4741 tcaagccact ggaaagctcg tacaggtctg ccccccagat atgccagtaa aactggctgg
     4801 gcttaatgcc accactcagc agagccagcc acagtgtggg attgaactga ccgtggaaga
     4861 aaaaacgctg ctgacggtta gcttttgctt tcgcgatgac cgcttccgcc agcgattttt
     4921 tcccagggaa aaattgcaca gaaagcgccg gacagctatc acttaagccg tcgtccttgc
     4981 caacaaccat aaactcgcgc gcgtgctcgc tcgtcgcggc cagcgcgtca ttgaaaaacc
     5041 gcaaaacggt tcggttatgg tgagggatat ccgatcccag tacgtgaatc agtacagtca
     5101 tgcccgccta cgccagagta aaaacacgcc acaacaaaga gaaaaataga cgatataggt
     5161 tgccatatat gcctgtgccg cgcccagcgc gccgtgcgta gggattagcc agtgggcaaa
     5221 taccatcaat aaagtgaact ggctgacttc cgccagaata taaaaccgca gtgaagcttt
     5281 ggcgatcacc agataaccaa agacataagc gcccactttt aacacatcac ccaccaactg
     5341 ccaggcaaag agatcgcgca tagcggtaaa tttattcgac aacagcagcc agatagcaaa
     5401 atcacgcagc aaccagacgg taaaactcgc cgccgccact gccggtaaga cgaatttcag
     5461 cgatttaacc acttcccggg tgatatcgcg cttttccgtt agccgcgaca acgtgggcag
     5521 caaatatacg ctgaacgatg ccgtaataaa ttgcaggtag gcatcggaaa tactgctcac
     5581 cccttgccag atccccacct catcccagct atactgcgcc gccagcagtt tacgcatcat
     5641 gatgtaagca acaggcaagg tcaccgacgt aatcaacgtc atgagcgtaa atttgctcaa
     5701 ctgccctgcc agaccgttat cccagctggg tttcagatag cttaacggga tagcaccacg
     5761 cttaattaac atgacggcgg caggaatcac caccagcgcg ggaatcaacg ccagacccag
     5821 caacgcccct tcatatccgc ccaaacggta actgacgtag tacgcaagaa cgccaatcaa
     5881 gctgccaaca atcagagata acgcattacc tgcggcatcg cgaaagcctt tcatcaacgc
     5941 cagtaacagg ttgccccagg cgatccccat ttgcaccagc gccactaaac gcaccagccc
     6001 ctgatagtcg tgattaccaa acaatcccaa gctgattggc gcagctgcca gcacaaaaac
     6061 cagcgccatc agcgtagaga agccaagtac catcgctgat gaagtgccga ccacgcggcg
     6121 cagctgttgc ggattatcat ggtactgggc aacgtatttg gttacaccgt taaagatgcc
     6181 agccccggca agcacgccga gcacggtaat caactggcgg aaatttgccg ccagcccaag
     6241 ccccgccgga ccaaatgaca ccgccagcaa cttaccgacc agtaacccgg caccaatctt
     6301 gaccagtgta ctggccgccg tccacaagga cgcttttgcc aacgacatat caggagaagt
     6361 agttcaacaa agtcgcaatt accgtacgct gattgacggg cgacaggttg tagaacagcg
     6421 gcaggcgcag caggcgctcg ctctctttgg tggtgtagcg atcttcaccg tggaactcac
     6481 caaagcgttc ccccgcaggg caaccgtgca gcggaatgta atggaacacc gccatgattt
     6541 ccgcttcttt cagaaagtta atcaacgcgc tccggtcacc aatatcccgc agtttaatgt
     6601 aaaacatatg cgcgttctgc aagcagccat cgggaatcga cggcagctcg atacgcccgg
     6661 ctttcgccag aggtgctaac gcatcgtagt agttttgcca cagcgccaga cgttgctggt
     6721 tgatacgatc cgctgcttcc agttgcgccc acaggtacgc agcttgcaga tcggacatca
     6781 aatagctgga gccgatatcg cgccaggtat atttatcgac ctgaccacgg aagaactggc
     6841 tgcggtttgt gcctttttca cggatgatct cggctcgttc gatcaacgct ttatcgttaa
     6901 tcagcgtcgc gccgccttca ccgcccgccg tgtagttttt ggtttcatgg aagctaaagc
     6961 agccaatatg accaatggtt cccagtgcac gccctttgta agtggacatc acgccctgag
     7021 cagcatcttc caccacaaac agattatgct ttttcgccaa cgccataatg gtgtccattt
     7081 cgcaggccac gcccgcgtaa tgcaccggca cgataacgcg cgttttgtcg gtgatcgccg
     7141 cttcaatcaa cgtttcgtcg atgttcatgg tgtccgggcg aacatccaca aaaacgattt
     7201 ttgcgccacg cagcacaaag gcattggcgg tggagacaaa ggtgtagctc ggcatgatca
     7261 cttcatcgcc aggctggata tcgagcagca gcgccgccat ctccagcgaa gcggtgcagg
     7321 acggcgtcag taacactttg gcgctgccaa aacgttgctc cagccactgc tggcagcgac
     7381 gggtaaaacc gccatcgcca cacagtttgc cgctacccat tgccgactgc atatagtcga
     7441 gttcggttcc caccaccggc ggtgcgttaa atggaatcat gtgatcacct gtataaccag
     7501 tacgcggtgc tttctacatt cgcaccactt tgtatgtatc gtttaagcgc ggcggtgttg
     7561 cccatttggg tcgccacccg caaagttgtt ttaccgcgag catacgccca gtttagcgcc
     7621 gtttgcatca gctcagcacc tgcaccgcgt ccagccagca ggccaattcg cgcatctgta
     7681 gcattgagtt cccgtaaaga gacataaccg cgaatatcgc cggacgccgc acgtaaaatc
     7741 agacattgat gatcaaaggt gccgcgcacg gcattttcaa tccactgtgc ataaaagcga
     7801 ctgctggcgt caggcgcata ccacggcgca cgaaaacggc tttgcgcaaa tgcggcgctg
     7861 gctaactgac gtaatgcggg aatatcggtc tcttgtgcca ctacagcacc gctatcactg
     7921 gcattgttca cgggtagcgc caaatcaact tcaccttcta ccagggagaa tcccagctgt
     7981 tgcagggcat ccagttcccc cgtatttgat gccgcaattt tggcctgcac ccgtgaccac
     8041 ggcgctaacg cgtctggcgt caggaacggt gcttcagacg taatgcgcac gatggcgctg
     8101 ttaacaccaa agaaggcgtt ttcccaggtt agtggctcaa tactggcgcg gacgggcacg
     8161 aagtaactcc agcagatatt ggccgtagcc agttttcgct aatgaactgg cagcacgctt
     8221 cacaccctcg tcatcgagcc agccgttacg ccaggcgatc tcttccaggc aggcaatctt
     8281 aaagccctgg cgtttttcca ccgtctgtac aaaggtgctg gcttcaatca ggctgtcgtg
     8341 agtgccagtg tccagccagg caaatccgcg cccgagcagt tcaacggtca ggttgcccgc
     8401 ctcgaggtac atctggttga tggaggtaat ctccagttca ccacgctccg acggcttcac
     8461 ctgctttgcg tactccacga ctttactgtc gtagaaataa agcccggtca ccgcccagtt
     8521 tgatttcggc tgttttggct tttcttccag cgagatagcg cggaaattat cgtcaaactc
     8581 caccacgcca aagcgttccg ggtccatcac ctgatagcca aaaaccgtcg ccacttccgt
     8641 gcgcgccgca acatgacgca gcttcggact gaagccctga ccgaagaaga tgttatcgcc
     8701 cagcaccaga caagaaggtt caccattaag gaaggtttca ccgatgataa aggcctgcgc
     8761 cagaccgtcc gggctgggct gttcggcata ttccagctgg ataccgaact cactaccatc
     8821 gcccagcagg cgctggaaat aacctttatc ttccggcgta gtgatgatga gaatttcgcg
     8881 gataccggcc agcatcagca ccgacagcgg atagtaaatc attggcttat cgtaaatcgg
     8941 caacagttgc ttcgatacgc cgcgcgtaat cggatgcaat cgggtaccgg aaccgcccgc
     9001 caggataata cctttcatgt ttgcctccgc tgaaaattag cctttcagac ctaaacgctc
     9061 gccctgatag ctgccgtcct gcacctgctt cctccagctt tcattagcca gataccactg
     9121 caccgtttta cgcattccac tttcaaaggt ttcctgcggc agccagccaa gttcacgggc
     9181 aattttcgaa gcatcaatgg catagcgcag atcatgcccc ggacggtcag cgacaaaggt
     9241 gatcaagtca cgataatgca ccacgccttg cggcttgttc ggagccagtt cttccagcag
     9301 ctcgcaaatg gtttccacaa catcgagatt cttacgctcg ttgtgaccac caatattata
     9361 ggtttcaccg actttcccgg tggtcgccac gcaatacagc gcgcgggcgt gatcttcaac
     9421 atacagccag tcacggattt gctgcccgtt gccatatacc ggtagcggtt tacccgccag
     9481 cgcgttgagg atcatcagcg ggatcagttt ttccggaaag tggtaaggac cgtagttatt
     9541 cgagcagttg gtgataagcg ttggcagacc ataggttcgc agccaggcgc gcaccagatg
     9601 gtcgctgctg gcttttgacg cggaataagg gctgctcggc gcatacggcg tggtttcggt
     9661 gaagaaatca tccgtcgagt gcaggtcacc atatacttcg tcagtggaga tatgatgaaa
     9721 acggaacgct gattttttat cttccgtcag cgcattccag taagcccgcg ccgcttcaag
     9781 caatgtataa gtcccgacaa tgttggtttc aataaacgct gccgggccgt caatagaacg
     9841 gtcaacatgg ctttcggctg ccagatgcat gacacagtct ggctgatgct cagtgaatac
     9901 gcgcgccagt tctgcccgat cgcagatatc aactttctca aaggcaaagc gctcgctttg
     9961 cgcgaccggt gccagcgaca tcaggtttcc ggcgtaggtc agcttatcga ccactaccac
    10021 cgcgtcgctc gtttcgttga tgatataacg caccagcgcc gagccaataa acccggcacc
    10081 gcctgttatc agaatctttc tcatcagcgc cagactcctt tagcatcgac gacatactgc
    10141 tgatggacat tgtcgccatt gataactttg aactgactat gatcgaccag catcaccagc
    10201 acgtctgccg ttgccagcgc ctcgtcaagc tgcgccagag tacaaagccc ggtcagtttt
    10261 ttcggcaact ggtggatgtt aggctcaaca accagagttt cgccgctatg ccactgggcg
    10321 atcagttcag cgatttccat cgccgggctt tcgcgcaggt catcaatatt cggtttaaac
    10381 gccagaccaa agcaggcgat tttcagttca ctggcgcgtt tatcggtagc cgccaggcaa
    10441 tcagccaccg ccgctttcac ctgatcgata acccagaacg gtttgtgatc gttcacttcg
    10501 cgcgcggtac ggataagccg cgcctgctgg gggttctgtg ccacgataaa ccacggatca
    10561 acagcaatgc agtgaccgcc cacgccaggg ccaggctgaa gaatattaac gcgagggtga
    10621 cgattcgcca ggcgaatcag ttcccagacg ttaatcccct gatcggcaca aatcagcgac
    10681 aattcattag caaaagcgat attcacatcg cggaagctgt tttcggtgag cttacacatt
    10741 tccgccgtcc gcgagttagt gacgacacac tcaccttcga ggaaaatttt gtacagttcg
    10801 ctggcgcggg ccgaacaaac cggcgtcata ccaccaatca cgcgatcgtt tttaatcagc
    10861 tcgaccataa cctggcccgg taacacgcgt tccgggcagt aagcaatgtt gacgtccgcc
    10921 tgctcgccca cctgctgtgg gaaagtgaga tccggacgca tctctgctaa ccattctgcc
    10981 atcttctcgg ttgaccccac cggcgaggtg gattcaagga tcaccagcgc gccttttttc
    11041 agcactggcg caatggagcg agcagccgat tcaacgtagg tcatatctgg ctcatgatcg
    11101 cctttaaacg gcgtgggtac agcaatcagc caggcatccg cttcaactgg cgtcgtgctc
    11161 gctcgtaaaa aaccgccttc tacggcagtt tttactacgc tcgccaaatc aggctcgacg
    11221 atatggattt cgccacgatt gatggtatca accgcgtgtt ggttgatatc gacaccaatc
    11281 acctgttttt gccgtgaggc aaacgctgct gctgtaggca gcccgatgta acccagtccg
    11341 ataacagaaa tggtcgcaaa actcatagtg atatccgatt attttttaac gcttccagaa
    11401 tgcgagagca tgcctgacca tcaccatacg ggttatgggc gcggctcata gtttgatatt
    11461 cgttttcgtc ttttaaaaga cgcgtcactt cctcgacaat tcgctgctta tccgtgccta
    11521 ccagacgcac cgtacccgcc gtcaccgctt ccggacgctc agtggtatcg cgcatcacca
    11581 gcaccggttt ccccagcgaa ggcgcttctt cctgaatgcc gcctgagtcg gtcaaaatca
    11641 gccaggcgtg gttcatcagc cagacaaacg gtaaatactc ctgaggatcg atcagaatga
    11701 catttttcac atgccccaga atgcgattga ccggctctct gacgttcggg ttgagatgca
    11761 ccggatagac aatctggatg tcctggtgcg tggtggcgat gtctgccagc gcgtggcaga
    11821 tttcttcaaa gccacgaccg aagctttcac gcctgtgacc ggtcaccaga atcatctttt
    11881 tatcggggtc gataaacggg taatttgccg caagttctga acgcagcgtg tcgctgctca
    11941 tcacctggtc acgcacccat aacagtgcat caatgactgt attaccggta atgaagattc
    12001 ggctatctgc aacgttttca cgcagcaagt tttgccggga agtttcggtt ggagagaaat
    12061 gatacatcgc cagatgcccg gtcaatgtac ggttagcctc ttccggccac ggcgaataga
    12121 gatcgcccgt gcgtaaacca gcctcaacgt gaccaacagg aatacgctga taaaacgccg
    12181 ccaggctggt tgccagcgtc gtcgttgtgt cgccgtgaac cagcacgacg tctggtttga
    12241 actcggcaag aataggtttt agcccttcca gaatccgaca ggttatctct gtcaggccct
    12301 gtcctggctg cattatgttg agatcgtagt caggtacaat ggaaaagagt ttcagcacct
    12361 gatcgagcat ctcccgatgc tgcgcagtga cgcaaacttt agcctcaaaa aaaggatctt
    12421 ttgccaacgc atgcaccaac ggagccatct tgatggcttc cgggcgcgta ccaaatacag
    12481 tcagtacttt cacatcgatt ctcttcgaat aagcggcgag cgcctttgcg ctcaccgcag
    12541 cagtgttgct atttcgagca acggcgggtt aatgcgacac cagccccgat cagccccccg
    12601 acaatgcccc acataatcat caggaaggca cgacgtgggc tatcgcgttt taccggttct
    12661 tccggcgtac gcaaatagcg ataggtctga aaacgcggat ccagggttgg accaacattc
    12721 agggtgttta acatggcccg attctgatca tagtcgagat caaaggccgg accgacggcc
    12781 tgtaaatttt ccagtcgagc ctggagcatt ggacgcccaa gcaggaacat ttctgaatca
    12841 ggtaattcct cggcaggcac atctgtcgca ctgcgcgaaa tattatgctg ctcagcaatt
    12901 ttcagcgcct gctcaatgct gttcatccgg cggtcgtaga tggctttcgc cacctcttcc
    12961 tgacgcttca cctgtgcttt catctggatg gtacgcgccg cccatgcgcc tttcagctca
    13021 tcattcagat ggctggctgc acgctggctg gcaaaagcaa catactgacg taacaggtta
    13081 ttagcgtcag gcgcggtttc ggcaataagc ttcacgctgt cattgaccgc gcgggtaaag
    13141 tctccgggga taaactggat gttgttaatc atttcatcca gcaacgccgc atcggctttg
    13201 ctgttgccca ccatccgctg cttgtaatag tcggtttgca gccagaactc tctgcgggta
    13261 tcccacgagg ccagttgcat aacaaactct ttataggctt cgtccatgac cgatggttgg
    13321 tcggcagaag ccatgtttga acggacatcc aggttacgca aaaattgctg ctgcgagtaa
    13381 tatcccccca gcatattcac cgttggacga tcggtaatcg ccgtcgagct ccactcctga
    13441 cgagcaaaaa acgtatacgc cagcgcgatt aacgcaaacg ccagccccat gccaataatc
    13501 catagcttcc cagcccacaa ggtacgaaac aacccacgaa tatccagttc attttcagcg
    13561 tcttcggccg gtttcccagg cattggttgt gtcatcacat cctcatttat ttggttaaat
    13621 tggggctgcc accacgattt ctacgcagtc tgcgttttac gcgcttaata aagcgagcaa
    13681 ctttccaggc acgcttaatg caatatccat agaggaggaa tgctagcaaa aagagcacca
    13741 acatgaccca ctccgggaca aaatgagaat attctgccag cacgccaatg gaagcgagca
    13801 gtgctgcggc aagggtaatc agcacaaacg cctggcggga agtaaacccg gcacgcatga
    13861 tcaaatggtg aatatgctga cggtcaggag agaatgggct catgccttta cgcaggcgac
    13921 ggtacataat cgccaccata tccattagcg gaatggcgat tatccacaaa gcggtaaccg
    13981 ggctgatggg atgggttttg ccttgggtcg tttcgagcag gatccagatc acggtaaaac
    14041 caatcagcgt actgcccgca tcacccataa agactttgta gcggcgaccc aggataccaa
    14101 ggttaagcat gatatatggc aggatggcgg cgatcatcgc aaagcaccag attgcgaggc
    14161 tggtttgccc gtcgaaccac aaaatcatac cgattgctgc aaacgagacg caggacaacc
    14221 cgcccagcaa gccatcaatg ccatcaacca tgttgaacgc attaatggcc gcccagacgg
    14281 caaatagcgt caggaagtaa ccaaacggtc cgagcaccat ctcccaggag ccaaagatat
    14341 aacccaggct actgagataa aggttgccaa acaccatcat aacaatgcca acagcggcct
    14401 gtatggtggc acggattttt acgctgatat caaaacggtc atccagcgcg ccaatgaaaa
    14461 caagcacacc ggcacaagcg agatagagag atgcatgcgg aatatagtaa tcgacaattc
    14521 cgaacgtgaa gcaaatccct gcgtaaaccg aaataccccc aacgagtggt atcaacccct
    14581 ggtgacgttt gcggaagttt ggtttatcca ctaaaccgac tttttttgcc accttacggg
    14641 caaaaaacag aaacagtgtc gtgaataaaa aaatactgat gagatcagta ctcactgtca
    14701 gtaaattcac aatgcatgct ctcagagaaa attattagca gaagtataac cacgaagacc
    14761 tttattcaga agggaaagtc cgttccgaac ccaccagtcc tgtgcaaaaa ttaaccattt
    14821 gatatgcaaa ttgcataaca tgccagcaaa ttccaggaat tttcctaaca tcgccagcgc
    14881 ggcattaaga ttacagatat aaaagcaaaa cgccacgtaa acacgtggcg tttttggcat
    14941 aagacaaatt tatgagcgtt tcatcatttc gaagaaatcg tcattggtct tggtcattgc
    15001 cagtttatta atgaggaatt ccattgcatc gatttcgccc atcgggtgaa tgattttgcg
    15061 caggatccac attttctgca gttcttcctg agtcgtgagc agctcttctt tacgggtacc
    15121 ggaacggttg tagtcgatag ccgggaagac gcgtttttca gcgatcttac gagagaggtg
    15181 cagttccatg ttgcctgtac ctttaaactc ttcgtaaata acttcgtcca ttttagaacc
    15241 ggtatcgata agcgccgtcg cgataatggt caggctgccg ccctcttcca cgttacgcgc
    15301 cgcaccgaag aagcgtttcg gacgatgcag ggcgttggca tccacaccac cggtcaacac
    15361 tttacctgac gccggaacaa cggtgttgta agcgcgcgcc agacgagtga tggagtcgag
    15421 cagaatgata acgtctttct tgtgctcaac caggcgtttc gccttctcga tcaccatttc
    15481 cgcaacctga acgtggcgag atgcgggttc gtcaaaggta gaagcaacaa cttcaccttt
    15541 taccagtcgc tgcatctcgg ttacttcttc cggacgttcg tcgatcagca gaaccatcag
    15601 cacacaatcc gggtggttgt aagcaatgct ctgagcaatg ttctgcagca gcatggtttt
    15661 accggctttc ggcggtgcca caatcagacc acgctgacca cgaccgatag gtgatgccag
    15721 atccagtacg cgagcggtta aatcttcagt agaaccgtta ccacgttcca tacgcagacg
    15781 agagtttgcg tgcagcgggg ttaagttctc aaagaggatt ttgttgcggg cgttttcagg
    15841 tttgtcgaag ttaacttcgt taactttcag cagcgcaaaa tagcgttcac cttctttcgg
    15901 cgggcgaatc ttaccagaga tggtatcacc agtgcggagg ttgaaacggc ggatttggct
    15961 aggggaaacg tagatgtcat caggaccggc gaggtaggag ctgtctgcgg aacggaggaa
    16021 accaaatcca tcctgcaata tctccagtac gccatcacca aagatatctt cgccactctt
    16081 tgcgtgctgc ttcaggatgg caaaaataat gtcctgctta cgcatacgag ccaggttttc
    16141 cagccccata ttttcgccga gagtgatcag ctcagaaacc ggcgtattct ttaattcggt
    16201 aagattcata gtggtgtgag ttcttaaact tggggtaaat ctcgaactta atgttgtgaa
    16261 tggtatggca ggatcatcca tgcctgttta cggtcatcga ctcatgtctt ttcgctgcct
    16321 ggtcacagga gagtacgcag aactgaaacg acaagacgga ttgagtgacg agccagaaat
    16381 ctgttcactt cgcatttaag ataaaacggg aagcgttgga aattacaaga ttcaaactta
    16441 ataaggtatg tttaatacga agtcaacact aagttagcat gactcacgcc gggcgtccag
    16501 tttttagcga cggggcaccc gaacatgaaa ttcccttacg ccaggttagc gtcgaggaac
    16561 tctttcaact gacctttaga cagtgcgccc actttggttg ccgccacttc accgtttttg
    16621 aacagcagca gagtcgggat accacggatg ccatatttcg gcgcagtgcc agggttttga
    16681 tcgatgttca gttttgcaac ggtcagtttg ccctgatatt cgtcagcgat ttcatccaga
    16741 atcggggcga tcattttgca cggaccgcac cactctgccc agaaatcgac gaggatcgcc
    16801 ccgtccgctt tgagtacatc cgtgtcaaaa ctgtcgtcag tcaggtgaat aattttatcg
    16861 ctcatatata actccacagg aataagcctg gcgtgttggt ttcgttgttg gtgtaacatt
    16921 aaccaactaa aggttgactt tatttcaccg gatacgcttt cgtaaagcaa tagtaagctg
    16981 atattctacc acactatgag caaaacacat ttaacagaac agaagttttc cgacttcgcc
    17041 ctgcatccga aggttgtaga agcccttgaa aaaaaagggt ttcataactg tacgcccatt
    17101 caggcactgg cccttccgct gacgctggcg ggtcgtgacg tagccgggca ggcgcaaacc
    17161 ggtaccggga aaacgatggc gtttcttacg tcaacgtttc attgtcttct ctctcatccc
    17221 gcgattgccg atcgcaaggt gaatcagccg cgtgccttaa ttatggcacc gacgcgtgaa
    17281 cttgccgtgc agatccatgc cgatgcggaa ccgctggcgg aagctaccgg cctgaagctg
    17341 ggtctggctt acggtggtga tggttacgac aaacagctga aagtgctgga aagcggcgtt
    17401 gacattctga ttggcaccac ggggcgttta attgactacg ccaagcagaa ccacattaac
    17461 ctcggtgcca ttcaggtagt ggtactggac gaagccgatc gcatgtacga tctgggcttt
    17521 attaaagata tccgctggct gttccgccgt atgccgcctg taaaccagcg cctcaacatg
    17581 ctgttctccg ccacgctttc gtaccgggta cgtgaactgg cgttcgagca gatgaacaat
    17641 gccgaatata ttgaagtgga accggaacag aaaacgggcc accgtattaa agaagagctt
    17701 ttctaccctt ctaacgaaga aaaaatgcgt ttgctgcaaa cgctgatcga agaagagtgg
    17761 ccagacagag cgattatttt cgccaatacc aaacaccgtt gtgaagagat ctggggccac
    17821 ctggcggcag atggtcatcg tgtcggtttg ttgacaggcg atgtcgcgca gaaaaaacgt
    17881 ctgcgtattc ttgatgaatt tacccgtggc gatctggata ttctggttgc caccgacgtt
    17941 gccgcgcgtg gtttgcatat tccggcagtg acgcacgtct ttaactacga tttacccgat
    18001 gactgtgaag attacgttca ccgtattggt cgtacaggtc gcgcaggcgc aagcggtcac
    18061 tctattagcc tggcgtgtga agagtatgca ttgaatttgc ctgctattga gacctatatt
    18121 ggtcactcaa ttccggtaag caaatacaat ccggacgcat tgatgaccga tctgccaaaa
    18181 ccgctgcgcc tcacgcgccc gcgcacaggc aatggtccgc gtcgtactgg cgctccgcgt
    18241 aatcgtcgtc gttcaggtta aataaaaaat gtcatactca aaatcattca aactgtatca
    18301 agatggcaag agaatgaatc ccgatgagct tactgtagta agtgattcag gtgaaagaat
    18361 gcagccaaca cagagacaga ttgaaggatg aagagtatgg gttccacctc atcgctgtat
    18421 gcagccattg atctcggttc gaatagtttt catatgctgg ttgtgcgcga ggtggctgga
    18481 agcatccaga cgctgacgcg aataaaacgc aaagtgcgtc tggctgctgg cctgaatagc
    18541 gaaaatgccc tctccaatga agcaatggag cgcggttggc aatgtttgcg cctgtttgct
    18601 gaacgtctgc aagatattcc tccctcgcaa attcgcgttg tcgctacggc gacgttacgc
    18661 cttgccgtca atgcgggtga ttttattgcc aaagcgcagg aaatcctcgg ttgtccggta
    18721 caggtgatca gcggtgaaga ggaagcacgt ctgatttatc agggtgttgc tcacaccact
    18781 ggcggtgccg atcagcgcct ggtagtggat atcggcggtg ccagtactga actggtaacc
    18841 ggcacgggtg cacaaaccac ctcgttgttc agcctgtcga tgggctgcgt cacctggctg
    18901 gaacgctatt ttgccgatcg taatctgggg caggaaaatt ttgatgctgc agaaaaagcg
    18961 gcacgcgaag tgttacgtcc ggttgccgat gaattacggt atcacggctg gaaagtgtgc
    19021 gttggtgctt ccggcaccgt gcaggcgtta caggaaatca tgatggcaca ggggatggat
    19081 gaacgcatta ccctggaaaa gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg
    19141 ctggaggaac tggagattga cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt
    19201 ctggcgatcc tgatcgccat ttttaccgaa ctgaatattc agtgtatgac cctggcgggc
    19261 ggtgcgctgc gtgaaggcct ggtctacggc atgttgcatc tggccgtcga gcaggatatt
    19321 cgcagccgta cgctgcgtaa tattcaacgc cgctttatga tcgatattga tcaggcacag
    19381 cgcgtagcca aagttgcggc taacttcttc gatcaggtgg aaaacgaatg gcatcttgaa
    19441 gcaataagcc gcgatttgct catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt
    19501 gacttcaaac aagcgccgca acacgctgct tatctggtgc gtaatctgga tcttcccggt
    19561 tttacccccg cacagaaaaa attgctggcg acgctactgc tcaaccagac taatccggtc
    19621 gatctctcat cgctgcatca gcaaaatgcc gtaccgccgc gcgtcgcaga acaactctgc
    19681 cgtttactac gcctggccat catttttgcc agccgtcgcc gtgacgatct cgtgccagag
    19741 atgacattac aggctaacca tgaactgttg accttgacgc ttccgcaagg ttggctaacc
    19801 caacatccgc tgggtaaaga gattattgct caggaaagcc agtggcagag ctatgtccac
    19861 tggccgctgg aagtgcatta atgagtaagt gccggatgcg atgctgacgc atcttttccg
    19921 gccttgatta tttccctcgt tttgccgcca tcatcgcttt cagattcgcc agatggcttt
    19981 gccctttctg catccgttct tcggcgctga ccactttgcg ctcctgttcc caaatcagat
    20041 catcctgcgg cagctccagc aaaaagcggc tcggctccgg gcgcaccagt tcgccgtact
    20101 gacggcgttc tttacacagc gtaaaggtca attccttctg ggcgcgggta atgccgacat
    20161 aggccagccg ccgctcctca tcgatattat cttcatcgat actgctctgg tgcggcaaaa
    20221 acccttcttc cataccgacc atgtagacat aaggaaactc cagtcctttt gacgcgtgga
    20281 gagtcatcag ttgcacctga tccagctctt cttcactctc accacgctcc atcatgtcgc
    20341 gcaaagtaaa gcgcgtcacc acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt
    20401 ccagcatctc cgtcatccag ctaaacagtt ggttgacgtt cttcatgcgc atttcggcgg
    20461 ctttcgggct gggcgatgtt tcgtacagcc aggattcata atccatgcca tggatcagat
    20521 cacgcaccgc agcaatcggc tcccgctccg ccaggcgctg gatttctgcc aaccagtgag
    20581 tgaagcgggt caatgcttca taaccacgtc cgctaagcgt ctgactcagg cccatatcaa
    20641 agctggcggt aaacatgctt ttattgcgcg tcatcgccca ctcacccagc tttttcagcg
    20701 tagccgggcc aatctctcgc ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt
    20761 ccgggttagt cagcacgcgc agataagcca gcaagtcctt gatttcagga cgagagaaaa
    20821 acgacgtacc accggatatt ttgtacggga tgcggttttg catcaggaat ttttcaaata
    20881 cccgcgactg atggttgccg cgataaagaa tggcgtaatc tttgtactgc gttttattga
    20941 cgaagtgatg ggcgatcagc tcgccagtaa cgcgctcggc ttcatgttcc tcgttattcg
    21001 cgcttaaaac ttttagctct gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga
    21061 cgtgcgggtt attggcgatc aggatgttcg ccgctttcag aatacgcccg gaagagcgat
    21121 agttctgctc aagcttaatc accttcagcg ccggaaaatc ctgactcagc agcaccaggt
    21181 tttgcggacg tgcaccgcgc caggagtaga tcgactggtc atcgtcaccc accacggtaa
    21241 agcgcgcgcg gctgcccacc agcagtttca ccagttcata ctggctggtg ttggtgtcct
    21301 gatactcatc caccagcagg tagcgaattt tgttctgcca gcgttcgcgt acttcttcat
    21361 tgcgttgcag cagcaacgtc ggcaataaaa tcaggtcatc gaagtcgaga acgttacagg
    21421 ctttcaggtg tgcatcatac agcccataac aatgggcaaa aatacgatcc cgctcgccct
    21481 ttgcctctgc cgccgcctgc gccggagttt tgagatcgtt cttccagtta gagatggtcg
    21541 aaatcagttg ttgcaggaga actttgtcat cttcaatcag cccctcggtc aactctttaa
    21601 gcaaagcaag ctgatcggta tcgtcaaata acgagaagtt cgctttcatc ccaagcgccg
    21661 catactcgcg tttgatgata tccagcccca acgtatggaa agtggagatc atcagcccac
    21721 gcgcctcttt acgccccagc gtctgcccta cacgctcttt catctcgcgc gctgctttat
    21781 tagtaaaggt caccgccgca atgtgccgcg cctgataacc gcaaccgcgg atcagatggg
    21841 cgattttatt ggtgataaca cgagttttac cggaacccgc gcccgccagc accaggcagg
    21901 ggccggtaac gaattcgaca gcttgttgtt ggccggggtt tagacgcata ggtgtattgc
    21961 tcaatcttcg aacgggggga ggattgtagc agaatcgcat ttgaatgtga ccgcgtgtaa
    22021 gccgctaacc ggttggctgc ggatgtttaa atcagagaga gaagaccgaa atcggagttc
    22081 atcgtgaatc cattagactt agaaaatatc gggtcgtaac gttgtgttat tactttttca
    22141 gagtttacta aggagaggtt tactcgtggc gattaatttc agccctaagg ttggtgaaat
    22201 actggaatgc aactttggta actatcctgt tagccaaaat ggaccgttca gcacgacgta
    22261 ttacgatggc cgcattcctc ctgaaatgat aaaaaacagg ttagttgtcg tgctcaacgg
    22321 aaaaattaat ggcaacgcat tcatcgtcgt tccattatca accactcgcg accatgacaa
    22381 gttaaaacgt ggaatgcatg ttgaaatagc cagtaatgtt atcaatgacc tgcaattctt
    22441 tgatcagcag attcgttggg ccaaaaccga cctggtacaa caagtcagtc gaaacagatt
    22501 aaacagagca agaacttaca gagggtatct taatcaatgt ttgccacatg agttggttgc
    22561 agacatacag agggcggtca tcaagtcaat caatgccata tcgctcataa attgacaata
    22621 aaatcaccat ccccgataat gactttgtag cccatagagg ccctaagttt tctccccttg
    22681 agggacctgc gaagcccgga ttttccgggc ttttgccata tatgtaccag cccctccctg
    22741 actgacaatt acgttagcat cggcggatca tgctaaaatc gccgcctgac aattattcac
    22801 tcaaggcacg atcatggcaa aaacagcagc agcactgcat atccttgtaa aagaagagaa
    22861 actggctctg gatcttctcg agcagattaa gaacggggcc gattttggca agctggcgaa
    22921 gaaacactcc atttgcccat caggcaaacg cggcggtgat ttaggtgaat tccgccaggg
    22981 tcagatggtt ccggcgttcg ataaagttgt gttctcttgt ccggtactgg agccaaccgg
    23041 cccgctgcac acccagttcg gttaccacat cattaaggtg ctgtaccgta actaatagca
    23101 aggccttctc cgggagaagg ccttgaactc tgaatcgcta ttaacccgca acagcaatac
    23161 gtttcatatc agtcatgtag ccgcgcagtt tcttacctac ctgctcaatc gcatggctgc
    23221 gaatcgcttc gttcaaatca cgcagttgcg cgttatctac cgcaccttcc ggaatagctt
    23281 tacccaaatc gcccggttgc agctctgcca taaacggttt cagcaacggc acacaagcgt
    23341 aagagaacag atagttaccg tactcagcgg tatcagagat aaccacgttc atttcgtaca
    23401 gacgcttacg ggcgatggtg ttggcaatca gcggcagttc gtgcagtgat tcatagtaag
    23461 cagattcttc gatgatgccg gaatcaacca tggtttcaaa cgccagctca acgcccgctt
    23521 tcaccatcgc gatcatcagt acgcctttat cgaagtactc ctgctcgccg attttgcctt
    23581 catactgcgg cgcggtttca aacgcggttt tgccggtctc ttcacgccag gtcagcagtt
    23641 tcttatcatc gttggcccag tccgccatca tgccggagga gaattcgccg gagatgatgt
    23701 cgtccatatg tttctggaac agcggtgcca tgatctcttt cagctgttct gacaacgcgt
    23761 aagcgcgcag tttcgccggg ttagagagac ggtccatcat cagggtgatg ccgccctgtt
    23821 tcagcgcttc ggtgatggtt tcccagccga actgaatcag tttttctgcg tatgccggat
    23881 cggtgccttc ttccaccagc ttgtcgaagc acagcagaga gccggcctgc aacataccgc
    23941 acagaatggt ttgctcgccc atcaggtcag atttcacttc cgcaacgaaa gaggattcca
    24001 gcacgcccgc acgatgacca ccggttgccg ccgcccatgc tttagcgatc gccatgcctt
    24061 cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt cggtacgccg aatccacgtt
    24121 tgtactcttc acgtacttcg gtgcctgggc atttcggcgc aaccattact acggtgatgt
    24181 ctttacggat ctgctcgccc acttcgacga tgttgaaacc atgcgagtaa cccagcgccg
    24241 cgccgtcttt catcagtggc tgtacggtgt gcactacatc agagtgctgc ttgtccggcg
    24301 tcaggttaac caccagatcc gcctgtggga tcagttcttc gtaagtaccc actttaaaac
    24361 cattttcggt cgctttacgc caggacgcgc gcttctcggc aatcgcttct ttacgcagag
    24421 cgtaggagat atcgagacca gaatcacgca tgttcaggcc ctggttcaga ccctgtgcgc
    24481 cacagccgac gatgaccact tttttaccct gaaggtagct cgcgccatcg gcgaattcgt
    24541 cgcggcccat aaagcgacat ttgcccaact gtgccagttg ctggcgcagg ttcagtgtat
    24601 tgaagtagtt agccatggtg attcctcgtg atgttgtgct tcttattgtt cggttcgcgt
    24661 ttgcaagatt gaattcacta tatgacagga aatttattgc ggaaattgat atattcacaa
    24721 catcatattg caatttttgc aacgtcaaca tcgagggctg tccctgtgga tttacgcgat
    24781 ctgaaaacct tcctgcatct ggcggaaagc cgccattttg gccgcagcgc gcgggcgatg
    24841 cacgttagcc catccacgct ctcacggcag attcagcgcc tggaagaaga tctcggtcag
    24901 ccgctgtttg tgcgcgataa ccgcacggtg acgctgactg aagcgggcga agaactgcgc
    24961 gttttcgccc agcaaacgct gttgcagtat cagcagctgc gccacaccat cgatcagcaa
    25021 gggccgtcgc tctctggcga attacatatc ttctgctcgg tgaccgctgc ctacagccat
    25081 ctgccgccga ttctggatcg cttccgcgcg gaacacccgt cggtggagat taaactcact
    25141 actggtgatg cggcagatgc aatggaaaaa gtggtcacgg gagaagcgga tctggcgatt
    25201 gccgggaaac cggagacgct acccggcgct gtagcgtttt cgatgctgga gaatctggcg
    25261 gtagtgctga ttgcgcccgc gctgccctgc ccggtgcgta atcaggtctc ggtagagaag
    25321 ccggactggt caacggtacc gtttatcatg gccgatcagg ggccagtacg ccgccgcatt
    25381 gaactgtggt ttcgacgcaa taaaatcagc aacccaatga tttacgccac ggttggcggg
    25441 catgaagcga tggtatcgat ggtggcgctc ggctgtggtg tggcgctgtt gccggaagtg
    25501 gtgctggaaa acagcccgga gcctgtacgc aaccgggtaa tgattttaga tcgcagcgat
    25561 gagaaaacgc cgtttgaact gggcgtgtgt gcacaaaaaa agcggctgca tgagccgctg
    25621 attgaggcgt tctggaggat tttgccgaac cacaaatgac gttgtcgcgc gagtaggcct
    25681 gataagcgaa gcgctatcag gcatttttcc ctaacccgcc aaaaagaacc tgaacgccgg
    25741 gttattggtt tcgtcgtggc aatcgtagcc cagctcattc agccgggttt cgaaatccgg
    25801 ttcatggtcg ccaagttcga acgccgccag tacgcgcccg tagtcggtgc catggctgcg
    25861 atagtggaac aaagaaatgt tccagtgcgt acccagcgtg ttgaggaagc gcagcagcgc
    25921 gcccggtgat tccgggaatt cgaagctgta gaggcgttcc tgcaacggat gtgacggacg
    25981 cccgccgacc atatagcgca catgcaactt cgccatttcg tcgtcggaga gatccaccac
    26041 gctgtagccg ccgtcgttga gcatctgcaa aatttctttg cgctcttcga ggccgcggct
    26101 caggcgcacg ccgacaaaga tgcaggcgtt tttggcatcg gcaaaacggt agttgaactc
    26161 ggtgaccgat cgcccgccaa gcagttggca gaatttgagg aagctgcctt tttcttccgg
    26221 aatggtcacc gccaacaacg cttcacgctg ttcgcccagt tcgcagcgtt ccgagacgta
    26281 gcgcaggccg tggaagttca cgttggcacc ggaaagaata tgcgccagcc gttcgccgcg
    26341 aatgttgtgc tgggcgatat attttttcat tcccgccagc gccagcgcgc cagagggttc
    26401 cgccaccgcg cgcacatctt cgaataaatc cttcatcgcc gcacagatcg catcactatc
    26461 gacggtgatg atatcgtcga gatactcctg gcacaaacgg aaggtttcgt cgccgatgcg
    26521 ttttaccgca acgccttcag caaacagccc cacgcgcggc agatcaaccg gatgacccgc
    26581 atccagcgct gctttcaggc aggcggagtc ttccgcttct acggcgatta ctttgatttg
    26641 cggcatcagt tgtttgatca gcaccgccac gccagcagcc agaccgccgc cgccgactgg
    26701 cacaaatacg cggtcgagat gggcgtcctg ctggagcagt tccagcgcca gcgtgccttg
    26761 cccggcaatc accatcggat ggtcgaacgg cggcacccag gtgaacccct gctgctgtga
    26821 cagttcgatc gctttggctt tcgcttcatc aaagttcgcg ccgtggagca gcacttcgcc
    26881 gccgaagccg cgcaccgcgt cgactttgat gtcggcggtg gcggttggca taacgatcag
    26941 ggccttcacg cctaaccgcg cagaagaaaa cgcgacgccc tgcgcgtggt tacccgcaga
    27001 agcagtgatc acgccgtgcg ctttttgttc ttccgtcagg cccgccatca tggcgtatgc
    27061 gccgcgcagc ttaaagctgt gcactggctg gcgatcttcg cgcttcacca gaatcacgtt
    27121 atcaagacga gacgagagct tttccatttt ttgtagcggc gtgacctgcg ccgcttcgta
    27181 gaccggcgcg cgcagcaccg ctcttaaata ttcagcacct tccggggcac cggacagggg
    27241 ttgcgagtca gccattatta accccccagt ttcgatttat cgcgcaccgc gcctttgtcg
    27301 gcgctggttg ccaggctggc ataagcacgc agggcaaagg agatctgacg ttcacgattt
    27361 ttcggcgtcc aggctttgtc accccgagct tcctgcgctt cacgacgcgc cgccagttcg
    27421 gcatcgctta cctgtaactg aatgccacgg ttcggaatgt cgatggcgat caggtcgccg
    27481 tcttcaatca ggccaatgct gccgccgctt gccgcttccg gtgagacgtg gccgatagaa
    27541 aggccagagg tgccgccaga gaaacgaccg tcggtgatca gcgcacaggc tttaccgagc
    27601 cccattgatt tcaggaagct ggttgggtag agcatttcct gcatccccgg accacctttt
    27661 gggccttcat agcgaattac taccacatct ccggcgacaa ctttaccgcc gagaatcgct
    27721 tctaccgcgt cgtcctggct ttcgtacact ttcgccgggc cggtgaattt gaggatgctg
    27781 tcatcgacgc ccgcagtttt aacgatgcag ccgttttccg cgaaattacc gtagagcacc
    27841 gccaggccgc cgtctttgct ataggcgtgt tccagcgagc ggatacagcc attggcgcga
    27901 tcgtcgtcca gcgtatccca acggcaatct tgcgagaatg cctgggtggt acggataccc
    27961 gcagggccgg cgcggaacat attttttacc gcgtcatcct gggtcagcat aacgtcgtat
    28021 tgttccagcg tttgcggcaa cgtcaggcca agtacgtttt tcacatcacg gttcagtaac
    28081 cccgcacgat ccagctcgcc gagaataccg ataacaccac cagcacggtg aacatcttcc
    28141 atatggtatt tttgggtgct cggcgcgact ttgcataact gcggaacctt gcgggaaagc
    28201 ttatcgatat cactcatggt gaagtcgatt tccgcttcct gcgccgccgc cagcaggtga
    28261 agtacggtgt tagtcgatcc acccatcgcg atatccagcg tcatggcatt ttcaaacgcc
    28321 gctttgctag caatattgcg gggcagtgca ctttcgtcgt tttgctcgta gtaacgtttg
    28381 gtcaattcaa caatgcgttt accagcattg aggaacagct gcttacggtc agagtgggtt
    28441 gccagcagcg agccgttgcc cggctgcgac agacccagcg cttcggtcag gcagttcatt
    28501 gagttagcgg taaacatccc ggagcaggaa ccgcaggttg ggcatgcgga acgttcaacc
    28561 tgatcgctct gggagtcaga tactttcggg tctgcgccct ggatcatcgc atcaaccaga
    28621 tcgagcttga tgatctgatc ggacagtttg gttttcccgg cctccatcgg gccgccggaa
    28681 acaaagatca ccggaatatt cagacgcagg gaagccatca gcatccccgg ggtgattttg
    28741 tcacagttag agatgcagac catggcgtcg gcgcagtggg cgttgaccat atactcaacg
    28801 gaatcagcga tcagttcgcg agatggcagt gaataaagca tccccccgtg gcccatggca
    28861 atcccatcat ccaccgcaat ggtgttgaac tctttggcaa cgccgccagc cgcttcaatt
    28921 tgttcggcga ccagtttacc gagatcgcgc agatggacgt gacccggtac aaattgggtg
    28981 aacgagttca caaccgcgat aatcggctta ccgaaatcgg cgtcggtcat tccggtggcg
    29041 cgccacagcg cacgagcacc cgccatatta cgaccatgag tggtggtggc ggaacggtac
    29101 ttaggcatac tttatttact cccagtgtct gtctcgtaaa tgggacggtg cgtgccgtcc
    29161 cattttttgt atttattgat taacttgatc taaccagccc catttatctt cggtttcgcc
    29221 ggtaaagagg ccgaagaagg cttgctgaat gcgtttggtg accgggccac aacggccttc
    29281 gccaacctga ataccgtcta cactgcgcac tggcgtgatt tctgccgccg taccggacat
    29341 aaagacttca tccgccaggt acaaggattc gcgcgacagc acctgctcac gcacttcaat
    29401 tcccagctct ttcgccagtt tgatgatggc atcacgggta atacccggca gcgcggagga
    29461 ggtgaacggt ggggtgaaca gcacaccatc tttcacttca aacaggtttt cgcctgcgcc
    29521 ttcagagata taaccgttca catccagcgc gataccttcc tgataaccgt ggcggcgcgc
    29581 ttcgctaccc accagcaggg aagagagata gttaccaccg gcttttgccg cagttgggat
    29641 ggtgtttggt gctgcgcggt tccaggagga aaccatcgca tcgatcccct gctccagcgc
    29701 ttctgcgccc agatacgctc cccacgggaa agcggcgata atcacatcgg ttgagtatcc
    29761 cgctggcggg tttacgccca tgccaacatc accaacgaag atcagcggac ggatataggc
    29821 gctggtgaga ttgtttttgc ggatcacgtc acggcaagct tccatcagct catcaatgct
    29881 ctgcgaaacc gggaagcgat agattttggc ggagtcatgc agacgctgca tatgctcacg
    29941 atggcggaat acaaccggtc ctttgtgcga gtcgtagcaa cggatgcctt caaaaaccga
    30001 ggtgccatag tgcagcgcgt gcgacatcac atgcaccttc gcgtcttccc agcgaaccat
    30061 ctccccattg aaccaaatgt aatcagcttt cttcgtggtc atttttatat tccttttgcg
    30121 ctcaggcgcg gatttgttgt gatgtggttg tgctctggca gatggcaacg tgtgcgacgt
    30181 ccaccagttt atttaactga ctaaacagta agtcgaccga ccgtgggctg gcaacggtca
    30241 attcgatatt tatattttgt gcatcgctgg cggcggccat attcattgag cagacgtgga
    30301 aaccacgatg acgcaccacg cgtaaaacac gttctaaggt ttccggattg aagcgagccg
    30361 atacattgac ctgatgttgc atcatgataa tttctccaac atttctgaat tactggcacc
    30421 aggcggcacc agcggccaga cgttctcaag ttcgtcgatt gagacatgaa gcaggtatgg
    30481 cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc tggtctttac gggtgatgtg
    30541 ttggccaggg atgccgaagg cgctggctaa cttgaggaaa tcggggttat cggtaagggt
    30601 ggtttcgctg tatcgttcct ggaaaaacag ttgctgccat tgtcgaacca tccctaaccg
    30661 ttggttatcg agtaagacga ttttcaacgg taactgcttg cgttttacgg tgcccagctc
    30721 ttgtacattc atcatgaaag agccgtcacc ggagatacag acgacggtat cgtttggtcg
    30781 cgcgacttgc gcgccaaccg ccgccggtaa accaaaaccc atggtgccta agccgctgga
    30841 ggtaatgaaa ttttccgggc gagtgtgtgc gatgtgctgc gcggcccaca tctgatgctg
    30901 ccccacatct gtggtcacaa cgcaatccgc aggtttacga tccgacagtt gttttaacaa
    30961 caacggcgcg tagatagcgt caccgggatg gtcgtaacgc caggcatgtt catcacgcag
    31021 ctgcgcgcag tattgctgcc agtcattgat atttaatggc tgctgtaatg ctggtaacag
    31081 cgcatttaaa tcaccctgta atgccacatg tgcctgacgc agcttgttca tttctgccgg
    31141 gtcgatatcc atatggataa cactggcgtg tggtgcgaag gtgttcaatt tgccggtcac
    31201 ccggtcatca aaacgtgcac ccacggcgat cagcaagtcg cactcctgca ccgcgaagtt
    31261 cgccgctttg gtgccatgca ttcccagcat gcccagatag tacggataat ctgcttcaac
    31321 tgcgcccagc cctttcagcg tgcaggtggc aggcattttt gtggtagcga gaaattctcg
    31381 taaagcagga actgcctgcg ccatacccac gccaccacca acgtacagca tcggtttttg
    31441 cgcttttgcc agcatctggc gcgcttgctc aacttcggca tgtgggaaag tcacttcgtt
    31501 ttcaacggtg gtgaaccacg gttccaggtc gccgctggct aactggatat cttttgggat
    31561 atcgaccaga accggaccag gacgacctga gctggcaacg tcgaatgctt cagccataat
    31621 gcgcggcaac tcttccagcg actgcaccag aaagctgtgc ttggtacagg ctaacgacaa
    31681 tcccaggaca tccacttcct gaaatgcgtc agtaccgata aacggtgcgg acacttgacc
    31741 ggtgatggca acaacaggga tagaatctaa cagtgcgtcc gcaagcccgg ttatcaggtt
    31801 ggttgcgccc ggaccagacg tggcgataca tacgccagtt ttgccggtag cacgggcata
    31861 accgatagcc gccattgccg caccctgctc atgtcggcac agcaagtgct ccacgccgcc
    31921 gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc ggataaccga aaacggtgtt
    31981 cacaccctgt gcccgcaacg catgtaccac ccactgtgcg ccattcatag ttagttcccc
    32041 gtcctgaatc ttgggaaaca gaattttgtg ctgttattca ttgtctgctc ctcggttatg
    32101 tttttaaggt caaaaaaaaa ccccggacct ttcggtgcgg gggtcttagt tcgttaaggc
    32161 ttgatctcta agcctttcct cgtccaagtg cagccccgca cggtgggata ataatcacca
    32221 ccacgctaat cacgaccagg ctaatcactc gtagaagggc tgtcattttg tcttttcttg
    32281 catcttgttc gaaggaatgc ctaaagagtt accatagatt ttgcgaatag cacaagatat
    32341 tttttggccg ttttttatga tggctttaat aatttcaata gaaattgttg ttttttaaaa
    32401 agaaaatagt gagtgaaaaa aattcaattt cagtacgaat gttgttccct ccctcgtagt
    32461 tcatcctttg tccgctgcat ttttgcgagc atcattccac cgccagaaca actcaacttc
    32521 acttgcacaa ataaacagga atcagccact aaaattcacg aattagcggg ctacagcagc
    32581 gatatcttat gcacataatg gtgactcaag gagggcttat gtcactgtca attgttcata
    32641 cccgcgcagc cctgggagta aatgcgcccc caatcactgt tgaggtacat atcagtaaag
    32701 gtttgcccgg cttaacgatg gtgggcttac cagaaacaac ggtaaaagaa gctcgcgatc
    32761 gcgtgcgcag cgccattatc aatagcggat atgaatatcc ggcgaaaaaa atcaccatta
    32821 accttgctcc agccgatcta ccaaaagaag ggggacgata tgatttacct atcgccattg
    32881 cgttgctggc ggcctcagaa cagcttacag ccaataagtt agatgaatat gaattagtcg
    32941 gagaactggc gcttacaggc gctctgcgtg gcgttcccgg tgcaatctcc agtgcaactg
    33001 aagctattaa gtcgggcaga aaaattatcg tcgcgaaaga taacgaagat gaagtggggc
    33061 taattaacgg tgaaggatgc ctggtagccg atcatctgca agctgtctgt gcgtttctgg
    33121 aaggtaagca cgctctcgaa cgcccgaaac caactgatgc agtatcccgg gcgctacaac
    33181 atgatctcag tgatgttgtc ggtcaggagc aaggaaagcg aggactggaa attaccgccg
    33241 ctggcgggca caacctttta ctgattgggc cgccgggaac aggtaaaaca atgctcgcca
    33301 gccgtattaa tggtcttttg ccagatttaa gcaatgaaga ggcactggag agcgctgcga
    33361 tattaagtct ggtaaatgct gaatcagtac aaaaacaatg gcggcagcgc ccgttccgct
    33421 cacctcatca cagtgcgtcg ttaacggcga tggtgggcgg tggtgcaatt ccagggccag
    33481 gtgaaatttc gttggcgcat aacggcgtgc tttttcttga tgagctacct gaatttgaac
    33541 ggcgtacact ggatgctttg cgagagccga ttgaatccgg gcagatccat ctttcacgca
    33601 cacgagcaaa aataacctat ccagcccgtt tccagcttgt tgcggcgatg aatcccagcc
    33661 ctaccggaca ttatcaggga aaccataacc gctgcacgcc agaacagaca ttacgttatc
    33721 tcaaccggct ctcggggccc tttctcgacc gcttcgatct ctcactggag atcccattac
    33781 caccccccgg cattttgagt aaaacggtag tgccgggaga aagcagcacc accgttaaac
    33841 aacgtgtaat ggctgccaga gagcgccaat ttaagcggca gaataagttg aacgcctggc
    33901 tggatagtcc ggaaatacgc aaattctgca agcttgagag cgaagatgcg cagtggctgg
    33961 aagaaacgct gatccatctg gggttatcga ttcgtgcctg gcagcggtta ttgaaagttg
    34021 cacgaaccat tgctgatatt gatcagtctg acattatcac acgtcagcat ttgcaggagg
    34081 cagttagcta tcgtgcgatt gaccgtttgc tcatccatct gcaaaaacta ctgacataaa
    34141 aaaagggcat ttcgcccttt ttattaatcg tcagaatcgg tgtagtcttc agcaccttca
    34201 acctgcggtt taccgccgga aagggtgtga aaacgttttg gacgcttgat acgcgtcata
    34261 tacttggacc acacgcgttc tgcttctgtc actggctcac gttcgccacg gcatactgct
    34321 acgaagagtt tctcttcctc ggtaaccggc tcgcgtttgc caagatccaa ctcattgaag
    34381 gcataaccat gacgctcaag cagttgtgcc tctttgatgg tgaaatcacc atggcgagag
    34441 aatccacgtg gataatgttt attgtcgaaa tatcgattag tcgtcgtaaa gctttccgcc
    34501 atcctgcacg ctcctaattc tttgaccgag ctagttatgg cgcggagtat tagttacgct
    34561 tgacagagtg taaaacaaaa catttaaatc ataacgacaa ataattttgc ggagagcact
    34621 gtggatacgg aattgttaaa aactttcctg gaagttagcc gaacgcgtca ctttggtcga
    34681 gcggctgaat cgctctatct gacccagtca gcagtgagct ttcgaatcag acaactggaa
    34741 aatcaactgg gtgtgaacct tttcacccgc cacagaaaca atatccgttt aaccgctgcc
    34801 ggtgaaaaac tactgcctta tgcagaaacg ctcatgagca cctggcaggc cgcccgtaag
    34861 gaggtggcgc atacctcacg acataacgag ttttctatcg gtgccagcgc ctcattgtgg
    34921 gaatgtatgc ttaatcagtg gctgggacgt ttgtatcaaa atcaggatgc ccatacaggc
    34981 ttacagttcg aagcgcgaat tgcccaacgg cagtctctgg taaaacagct gcatgaacgc
    35041 cagcttgatc ttcttattac cactgaagcg cccaaaatgg acgaatttag tagtcagttg
    35101 ctggggtatt tcactttagc gctttatacc agtgcccctt ctaaactaaa gggagatctt
    35161 aactatctgc gactggagtg ggggccagat tttcaacagc atgaggcagg tttgatcggt
    35221 gctgacgaag tgcccattct gacaaccagt tctgctgaac tggcacagca acagattgcg
    35281 atgcttaatg gttgcacctg gctacccgtc agctgggcgc gtaaaaaagg cggcctgcat
    35341 accgttgtcg atagcacaac actttcacgg ccgctttatg ccatatggct gcaaaatagc
    35401 gataaaaata cgttgattcg cgatctattg aaaattaacg tgctggatga agtgtattaa
    35461 tctgaatggc tggcaaggat gccggtagaa ggatttactt cagagagggt tatttcagat
    35521 aaaaaaaatc cttagctttc gctaaggatg atttctggca ggggcggaga gactcgaact
    35581 cccaacaccc ggttttggag accggtgctc taccaattga actacgcccc taattag
//
LOCUS       DAZKFZ010000026        34717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000026 DAZKFZ010000000
VERSION     DAZKFZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..646
                     /locus_tag="ACHOQW_001210"
                     /pseudo
     CDS             1..646
                     /locus_tag="ACHOQW_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
     gene            complement(796..2160)
                     /gene="mnmE"
                     /locus_tag="ACHOQW_001211"
     CDS             complement(796..2160)
                     /gene="mnmE"
                     /locus_tag="ACHOQW_001211"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9572314.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2266..3912)
                     /gene="yidC"
                     /locus_tag="ACHOQW_001212"
     CDS             complement(2266..3912)
                     /gene="yidC"
                     /locus_tag="ACHOQW_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9572315.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3915..4172)
                     /gene="yidD"
                     /locus_tag="ACHOQW_001213"
     CDS             complement(3915..4172)
                     /gene="yidD"
                     /locus_tag="ACHOQW_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9572316.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            complement(4136..4495)
                     /gene="rnpA"
                     /locus_tag="ACHOQW_001214"
     CDS             complement(4136..4495)
                     /gene="rnpA"
                     /locus_tag="ACHOQW_001214"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9572317.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4512..4652)
                     /gene="rpmH"
                     /locus_tag="ACHOQW_001215"
     CDS             complement(4512..4652)
                     /gene="rpmH"
                     /locus_tag="ACHOQW_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9572318.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4882..4962)
                     /locus_tag="ACHOQW_001216"
     CDS             complement(4882..4962)
                     /locus_tag="ACHOQW_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572319.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            5259..6662
                     /gene="dnaA"
                     /locus_tag="ACHOQW_001217"
     CDS             5259..6662
                     /gene="dnaA"
                     /locus_tag="ACHOQW_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9572320.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6667..7767
                     /gene="dnaN"
                     /locus_tag="ACHOQW_001218"
     CDS             6667..7767
                     /gene="dnaN"
                     /locus_tag="ACHOQW_001218"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9572321.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7767..8840
                     /gene="recF"
                     /locus_tag="ACHOQW_001219"
     CDS             7767..8840
                     /gene="recF"
                     /locus_tag="ACHOQW_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9572322.1"
                     /translation="MSLTRLLIRDFRNIETADLALCPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8869..11283
                     /gene="gyrB"
                     /locus_tag="ACHOQW_001220"
     CDS             8869..11283
                     /gene="gyrB"
                     /locus_tag="ACHOQW_001220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9572323.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11307..11405)
                     /locus_tag="ACHOQW_001221"
                     /pseudo
     CDS             complement(11307..11405)
                     /locus_tag="ACHOQW_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11523..11921
                     /gene="yidB"
                     /locus_tag="ACHOQW_001222"
     CDS             11523..11921
                     /gene="yidB"
                     /locus_tag="ACHOQW_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP9572324.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12036..12848
                     /gene="yidA"
                     /locus_tag="ACHOQW_001223"
     CDS             12036..12848
                     /gene="yidA"
                     /locus_tag="ACHOQW_001223"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9572325.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12894..13550)
                     /gene="yidX"
                     /locus_tag="ACHOQW_001224"
     CDS             complement(12894..13550)
                     /gene="yidX"
                     /locus_tag="ACHOQW_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP9572326.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13686..13799
                     /locus_tag="ACHOQW_001225"
                     /pseudo
     CDS             13686..13799
                     /locus_tag="ACHOQW_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13796..14860)
                     /gene="cbrA"
                     /locus_tag="ACHOQW_001226"
     CDS             complement(13796..14860)
                     /gene="cbrA"
                     /locus_tag="ACHOQW_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP9572327.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            14961..16175
                     /gene="yidR"
                     /locus_tag="ACHOQW_001227"
     CDS             14961..16175
                     /gene="yidR"
                     /locus_tag="ACHOQW_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP9572328.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16177..16509)
                     /gene="yidQ"
                     /locus_tag="ACHOQW_001228"
     CDS             complement(16177..16509)
                     /gene="yidQ"
                     /locus_tag="ACHOQW_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9572329.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16749..16821
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16815..17228
                     /gene="ibpA"
                     /locus_tag="ACHOQW_001229"
     CDS             16815..17228
                     /gene="ibpA"
                     /locus_tag="ACHOQW_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP9572330.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17340..17768
                     /gene="ibpB"
                     /locus_tag="ACHOQW_001230"
     CDS             17340..17768
                     /gene="ibpB"
                     /locus_tag="ACHOQW_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP9572331.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17964..19625
                     /gene="yidE"
                     /locus_tag="ACHOQW_001231"
     CDS             17964..19625
                     /gene="yidE"
                     /locus_tag="ACHOQW_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP9572332.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19622..20338)
                     /gene="yidP"
                     /locus_tag="ACHOQW_001232"
     CDS             complement(19622..20338)
                     /gene="yidP"
                     /locus_tag="ACHOQW_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9572333.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20634..22250
                     /locus_tag="ACHOQW_001233"
     CDS             20634..22250
                     /locus_tag="ACHOQW_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9572334.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22250..23572
                     /locus_tag="ACHOQW_001234"
     CDS             22250..23572
                     /locus_tag="ACHOQW_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFP9572335.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23569..24462)
                     /gene="yidL"
                     /locus_tag="ACHOQW_001235"
     CDS             complement(23569..24462)
                     /gene="yidL"
                     /locus_tag="ACHOQW_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9572336.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24629..26344
                     /gene="yidK"
                     /locus_tag="ACHOQW_001236"
     CDS             24629..26344
                     /gene="yidK"
                     /locus_tag="ACHOQW_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP9572337.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26341..27834
                     /gene="yidJ"
                     /locus_tag="ACHOQW_001237"
     CDS             26341..27834
                     /gene="yidJ"
                     /locus_tag="ACHOQW_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9572338.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27881..28330)
                     /gene="yidI"
                     /locus_tag="ACHOQW_001238"
     CDS             complement(27881..28330)
                     /gene="yidI"
                     /locus_tag="ACHOQW_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP9572339.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28439..28786
                     /gene="yidH"
                     /locus_tag="ACHOQW_001239"
     CDS             28439..28786
                     /gene="yidH"
                     /locus_tag="ACHOQW_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP9572340.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28776..29138
                     /gene="yidG"
                     /locus_tag="ACHOQW_001240"
     CDS             28776..29138
                     /gene="yidG"
                     /locus_tag="ACHOQW_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP9572341.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29135..29632
                     /gene="yidF"
                     /locus_tag="ACHOQW_001241"
     CDS             29135..29632
                     /gene="yidF"
                     /locus_tag="ACHOQW_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9572342.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29640..30824)
                     /gene="emrD"
                     /locus_tag="ACHOQW_001242"
     CDS             complement(29640..30824)
                     /gene="emrD"
                     /locus_tag="ACHOQW_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP9572343.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            30906..30980
                     /gene="ysdE"
                     /locus_tag="ACHOQW_001243"
     CDS             30906..30980
                     /gene="ysdE"
                     /locus_tag="ACHOQW_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP9572344.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(31104..31193)
                     /gene="tisB"
                     /locus_tag="ACHOQW_001244"
     CDS             complement(31104..31193)
                     /gene="tisB"
                     /locus_tag="ACHOQW_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP9572345.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31758..31856
                     /gene="ivbL"
                     /locus_tag="ACHOQW_001245"
     CDS             31758..31856
                     /gene="ivbL"
                     /locus_tag="ACHOQW_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP9572346.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31962..33650
                     /gene="ilvB"
                     /locus_tag="ACHOQW_001246"
     CDS             31962..33650
                     /gene="ilvB"
                     /locus_tag="ACHOQW_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP9572347.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33654..33944
                     /gene="ilvN"
                     /locus_tag="ACHOQW_001247"
     CDS             33654..33944
                     /gene="ilvN"
                     /locus_tag="ACHOQW_001247"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9572348.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34207..>34717
                     /locus_tag="ACHOQW_001248"
     CDS             34207..>34717
                     /locus_tag="ACHOQW_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572349.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTVLRKKLVN
                     SIVMKNTDFN"
BASE COUNT         8407 a         8753 c         9167 g         8390 t
ORIGIN      
        1 gccaaaatgt ttaaaaatga attaaataaa gaagatatat tgaaactgat ggcagcaaga
       61 gaaaaagtgg cgggagcatc agacaaatgg acgaaggcat caggcttata ttctgcgata
      121 gtgaaagggc atacgaagat tgttgctgcc tggatggaga cagctgaagt gatagccagc
      181 cattatgaaa atgataaaga tgtagtgaga gaactcctgt cgctgagcag aaataatgca
      241 gtttgctctt tgtatgttgc cagctataag acaatgagta agcaggtcat tgatgtatat
      301 ctgaatgcgg cgattcgcct ggcgttgcaa cacgggttca ctttcgatga gattttggag
      361 cagtttaccc gtgactttga tgggaagtca ttctctcttg cggtagagaa agcggatgat
      421 atatatgggt ctctggctga aaatattcaa aattgtggtt ggtgaaaatg aaaattatct
      481 taaagatgtg atgatgcaac tggaagcgaa gaataatgaa ggcaaatatg taataagcaa
      541 ggcaaatggc aatcctgtat ttaaagaact attctggaaa gcaatagacg aatttaactt
      601 tccgcaagag gagcttaatc ggctaaagca atatcgttct ctgtaaagac aggactgcaa
      661 gatgttgaaa gtcgctccag ccagtagtaa aggcagtttt ggtgcaggat gctcataaaa
      721 aaacaagcgc tgttccgttc tgatagttag tttcccccag aacggaacag tgccctttat
      781 atcaatttag acgacttact taccaataca gaagctggag aaaatccgcc ccagcaaatc
      841 atcagaggta aattccccgg tgatttcgct taagttctgc tgtgccaggc gcaactcttc
      901 cgccagcagt tcgcccgccc aggcacccaa cagttgcgct ttaccttgct gcaaatgctc
      961 cgctgcctgt tccagcgcct gaaggtggcg acgacgcgcc aggaagccgc cttccatgtt
     1021 ggtgtcaaag cccatgctct gtttgagatg gttacgcagc acgtccacgc cttcgccagt
     1081 ctttgccgag agacgaatca acgcatgacc gttcacttca ctcattccca gcgtttcgcc
     1141 agtgatatcg gctttattgc gtaccacggt gatcggcagt ttcgcgggca gacgggcgat
     1201 aaattccggc cagatctccg ccggatcaac ggcgtctgtc gtggtgccat cgaccataaa
     1261 cagcacgcgg tcggcttgtt caatttcttg ccacgcgcgc tcgataccaa tacgttccac
     1321 ttcatcgctg gcttcacgta gcccggcggt gtcgatgata tgcagcggca ttccgtcaat
     1381 gtggatatgc tcacgcaaca cgtcacgcgt ggttccggcg atatcggtta cgattgctgc
     1441 ttcacggcca gccagcgcgt ttaacaggct cgatttaccg gcattaggac gtccggcaat
     1501 caccaccttc atcccttcgc gcaacaaact accctgacgt gcttcagcac gcactgcatc
     1561 gagatcggca atcacgtcat taagctgggc ttcaattttt ccgtcggaga ggaaatcgat
     1621 ctcttcatcg gggaaatcaa ttgccgcttc gacgtaaatg cgcaagtggg tgagggcttc
     1681 caccagatgg ttgacccgtg cggagaatgc gccttgcagc gagttaagtg ccgaacgggc
     1741 tgcctgttcc gaactggcat cgataagatc ggcaatcgcc tcggcctggg ctaagtcgag
     1801 tttatcgtta agaaacgcgc gttcggaaaa ctcaccaggg cgagcaatcc gcaggccggg
     1861 aatggtcaga atgcgtttta acagcaggtc gagaatcacc ggaccgccat gaccctgcag
     1921 ttccagcaca tcttcgccgg tgaacgagtt cgggccaggg aaccatagcg caatcccctg
     1981 atcgagcacg ctgccatcgg cgtctttaaa cggaagataa tcggcgtagc gcggcttagg
     2041 cagtttaccc agcacggttt cggcaacttc acgggctttg aagccggaga tgcgcaggat
     2101 gccaacgccg ccacgtcccg gaggcgtggc ctgggctacg atagtgtcat tatcgctcat
     2161 gatgttcctg ttgctttgtg tggcggatac gcggtgctta tccgccctac gaaaagaaaa
     2221 aaggcggtca actgaccgcc cttattttag cgaaaactca ccgaatcagg attttttctt
     2281 ctcgcggcta tgcaggccac gtttttccag accacggtaa atcagctgct gctgaataat
     2341 ggttaccagg ttgctgacga tatagtacag caccagacct gacgggaacc acaggaagaa
     2401 cacggtgaag atgaccggca taaaggtcat gatcttctgc tgcatcgggt cggtcacagt
     2461 ggtcggcgac atcttctgaa tgaagaacat cgttacgccc atcaggatcg gcaggatgta
     2521 gtacgggtcc tgtgcagaca ggtcgtggat ccacagtgca aacggtgcct gacgcagttc
     2581 aacggaaccc atcagcatgt agtacaacgc caggaagatt ggcatctgga tcagcagcgg
     2641 gaagcagccg cccagcgggt taaccttctc agctttgtac agcgccatca tttcctggct
     2701 gatacgctgt ttgtcatcgc ccagacgctc acgcattgcc tgaatcttcg gctgcagcat
     2761 acgcatcttc gccatggagg tgtactgcgc tttggtcagc gggtacatga tgccacgaac
     2821 gataaaggtg atgatgataa tggagaagcc ccagttaccc acaaagctat ggatccattt
     2881 cagcagtttg aacagcggct gagagatgaa ccacaaccaa ccgtaatcaa cggtcagatc
     2941 caggtgcgga gcaacagctg ccattttgtc ctggatttcc gggccaaccc acagggtgct
     3001 gttcatcgcg ccagtctgac caggctgaac cagtaccggc tgagatttat agccgatagc
     3061 ggcgatgccg ttacccagat tagcagtata gaagttgttg gtaccgtcgt tatgcgggat
     3121 ccacgccgtc gcgaaatact gttgcagcat tgccacccaa ccacctttcg aagagatgtt
     3181 caggttttcg ttatcggcaa tggtatcgaa cttgtatttc tcgtacttct cgtcaggcgt
     3241 ggagtacgcc gcgccacgga aggtgtgcag tgcgaagttg ctgcttccgg tatcgagatg
     3301 cggtggcaga gtgatggatt gcttcaactg accaaaggtg gagatttcca gcggtttctc
     3361 gccagcgttc tgcacgttgt agttgacgtt gacagcgtaa tcaccccgtt tcaggacaaa
     3421 cgttttggta aacgtgttgc ctgccgcgtc ggtatacgtc atcggcacct gcagttcgtt
     3481 ttgaccttca gccagcacat aagcgtcttt ttcaacgtta tacagcggac gcgggccgtt
     3541 agccgggtta tctgggccat cacgaccggt cagaccgctc tgtgcctgat aaataaactg
     3601 cggtgaagtt tccagcagct ggaacggctg ggtagagttc agctctttcg ggtaagcagg
     3661 cagcagagct tgctcaacat caccaccacg ggtgttgatg gtcagatcaa gcacgtcggt
     3721 cttaaccgag atcagtttcc cctggccact ggccggtacg ccctggtcgg cggcgctacc
     3781 cgctgcggtg gtcgttgtct gcgtggtctg ttgggcctga ggttgcgggt ttttatcctg
     3841 ctcccaggct tgccagatca tgaaagacac gaacagcaaa gcgatgacta aaagattgcg
     3901 ttgcgaatcc atcgttagtg ttctctggta ttaaatggtc cgggcgggac gggatcgtca
     3961 ccaccagggt gtaaagggtg gcattttaat acgcgtttca ccgtcaacca actgcctttt
     4021 atcactccaa acctgcgcaa tgcctcaatt ccgtagcttg aacaggttgg agtgaaacga
     4081 caatgcggcc cgagtagcgg actaatcagg cgttgataga cccgaatgag ggctatcagg
     4141 acccgcgagc caggcgacag tggcggcgcc ataatttttc caacgcttcc gagagagcac
     4201 ggttatcgag gttggcaacc ccttttttcg ccaccaccac gaaatccata gccgggagtt
     4261 catgttggcg cagacggaag ctttcacgcg tcagacgttt aatccgattg cgttcatggg
     4321 cgcgtcgaac gtttttcttg gcgactgtaa gaccgatacg gggatgcccc agcgaattca
     4381 ggcggccgag aatggtaatt tgcggcgtgc cagcccgttg tggctgctgg aagacgaatg
     4441 tgaattgact gggagttaac aagcgtaact ccctgggaaa tgcgagctta accactcagg
     4501 ggttagcttt attacttaga aacggtcaga cgagcgcggc ctttagcacg acgacgtgcc
     4561 agaacctgac gaccattttt agtagccata cgagcacgga agccgtgaga acggttgcgc
     4621 ttcagtacag acggttgaaa agtgcgtttc atggcgattt ctacctaaac ttgaataaat
     4681 tcaatggctt tattggatat ccgccgaaaa atgaaacgat ggacaccgaa gccatgggtg
     4741 attaaagagg ccggattgta ataattgtac actccggagt caattctctt tccttattta
     4801 ccgcgctttt ccgcaccttt tcgcagggaa aacgtacggc ctcacaccag tgaaaaccag
     4861 catggcgcgc cgggtggagg attatacggg ctgatgggta aagcgcaagg atcgtcctgg
     4921 atctttatta gatcgattaa gccaattttt gtctatggtc attaaatttt ccaatatgcg
     4981 gcgtaaatcg tgcccgcctc gcggcaggat cgtttacact tagcgagttc tggatcatcc
     5041 tgtggataaa tcgggaaaat ctgtgagaaa cagaagatct cttgcgcagt ttaggctatg
     5101 atccgcggtc ccgatcgttt tgcaggatct tgatcgggca tataaccgca gacagcggtt
     5161 cgtgcgtcac cctcaagcag ggtcttttcg acgtacgtca acaatcatga atgtttcagc
     5221 cttagtcatt atcgactttt gttcgagtgg agtccgccgt gtcactttcg ctttggcagc
     5281 agtgtcttgc ccgattgcag gatgagttac cagccacaga attcagtatg tggatacgcc
     5341 cattgcaggc ggaactgagc gataacacgc tggccctgta cgcgccaaac cgttttgtcc
     5401 tcgattgggt acgggacaag taccttaata atatcaatgg actgctaacc agtttctgcg
     5461 gagcggatgc cccacagctg cgttttgaag tcggcaccaa atcggtgacg caaacgccac
     5521 aagcggcagt gacgagcaac gtcgcggccc ctgcacaggt ggcgcaaacg cagccgcaac
     5581 gtgctgcgcc ttctacgcgc tcaggttggg ataacgtccc ggccccggca gaaccgacct
     5641 atcgttctaa cgtaaacgtc aaacacacgt ttgataactt cgttgaaggt aaatctaacc
     5701 aactggcgcg cgcggcggct cgccaggtgg cagataaccc tggcggtgcc tataacccgt
     5761 tgttccttta tggcggcacg ggtctgggta aaactcacct gctgcatgcg gtgggtaacg
     5821 gcattatggc gcgcaagccg aatgccaaag tggtttatat gcactccgag cgctttgttc
     5881 aggacatggt taaagccctg caaaacaacg cgatcgaaga gtttaaacgc tactaccgtt
     5941 ccgtagatgc actgctgatc gacgatattc agttttttgc taataaagaa cgatctcagg
     6001 aagagttttt ccacaccttc aacgccctgc tggaaggtaa tcaacagatc attctcacct
     6061 cggatcgcta tccgaaagag atcaacggcg ttgaggatcg tttgaaatcc cgcttcggct
     6121 gggggctgac tgtggcgatc gaaccgccag agttagaaac ccgcgtggcg atcctgatga
     6181 aaaaggccga cgaaaatgac attcgtttgc caggcgaagt ggcgttcttt atcgccaagc
     6241 gtctacgatc taacgtacgt gagctggaag gcgcgctgaa ccgcgtcatt gccaacgcca
     6301 actttaccgg acgggcgatc actatcgact tcgtgcgtga ggcgctgcgc gacttgctgg
     6361 cattgcagga aaaactggtc accatcgaca atattcagaa gacggtggcg gagtactaca
     6421 agatcaaagt cgcggatctc ctttccaagc gtcgatcccg ctcggtggct cgtccgcgtc
     6481 agatggcgat ggcgctggcg aaagagctga ctaaccacag tctgccagag attggcgatg
     6541 cgtttggtgg tcgtgaccac actacggtgc ttcatgcctg ccggaagatc gagcagctgc
     6601 gtgaagagag ccacgatatc aaagaagatt tttcaaattt aatcagaaca ttgtcatcgt
     6661 aaacctatga aatttaccgt agaacgtgag catttattaa aaccgctaca acaggtgagc
     6721 ggtccgttag gtggtcgtcc tacgctaccg attctcggta atctgctgtt acaggttgct
     6781 gacggtacgt tgtcgctgac cggtactgat ctcgagatgg aaatggtggc acgcgttgcg
     6841 ctggttcagc cacacgagcc aggagcgacg accgttccgg cgcgcaaatt ctttgatatc
     6901 tgccgtggtc tgcctgaagg cgcggaaatt gccgtgcagc tggaaggtga gcggatgctg
     6961 gtgcgctccg ggcgtagccg tttttcgctg tctaccctgc cagcggcgga tttcccgaac
     7021 ctcgatgact ggcagagtga agtcgaattt accctgccgc aggcgacgat gaagcgtctg
     7081 attgaagcga cccagttttc tatggcgcat caggacgttc gctattactt aaacggtatg
     7141 ctgtttgaaa ccgaaggtga agaactgcgc accgtagcga ccgacggcca ccgtctggcg
     7201 gtctgttcaa tgccaattgg tcaatctttg ccaagccatt cggtgatcgt gccgcgtaaa
     7261 ggcgtgattg aactgatgcg tatgctcgac ggcggcgata atccgctgcg tgtgcagatt
     7321 ggcagcaaca atattcgcgc ccacgttggc gactttatct tcacctctaa actggtggat
     7381 ggtcgcttcc cggattaccg ccgcgttctg ccgaagaatc cggacaaaca tctggaagca
     7441 ggctgcgatc tcctcaagca ggcgtttgct cgcgcggcaa ttctctctaa cgagaaattc
     7501 cgcggtgtgc gcctgtatgt cagcgaaaac cagctgaaaa tcaccgccaa caacccggaa
     7561 caggaagaag cggaagagat cctcgacgtt acctatagcg gtgcggagat ggaaatcggc
     7621 ttcaacgtca gctatgtgct ggatgttctg aacgcgctga aatgcgaaaa cgtccgcatg
     7681 atgctgaccg attcggtttc cagcgtgcag attgaagatg cagccagcca gagcgcggct
     7741 tatgttgtca tgccaatgag actgtaatgt ccctcacccg cttgttgatc cgcgatttcc
     7801 gcaacattga aaccgcggat ctcgctttat gtcccggctt taactttctg gtaggtgcca
     7861 acggcagtgg caaaaccagc gtgctggaag ccatctatac gctcggccat ggtcgggcgt
     7921 ttcgcagttt gcagattggt cgcgtcattc gccacgagca ggaggcgttt gttctccacg
     7981 ggcgattaca gggcgaagag cgcgagacgg cgattggctt aaccaaagac aaacagggcg
     8041 acagcaaagt ccgcatcgac ggtactgacg ggcataaagt cgcggaactg gcgcacctga
     8101 tgccaatgca gctgataacg ccagaagggt ttactttact caacggcggc cccaaataca
     8161 gaagagcatt cctcgactgg ggatgctttc acaacgaacc cggatttttc accgcctgga
     8221 gcaatctcaa gcgattgctc aagcagcgca atgcggcgct gcgccaggtg acacgttacg
     8281 aacagctacg cccgtgggat aaagaactga tcccgctggc ggagcaaatc agtacctggc
     8341 gcgcggagta tagcgccggt atcgcggccg atatggctga tacctgtaag caatttctcc
     8401 ctgagttttc tctgactttc tctttccagc gcggctggga gaaagagaca gaatatgctg
     8461 aggtgctgga acgtaatttt gaacgcgatc gccagctaac ctacaccgcg catggcccgc
     8521 ataaagcgga cttacgcatt cgcgccgacg gtgcgccggt ggaagatacc ttatcgcgtg
     8581 ggcagcttaa gctgttgatg tgcgccttac gtctggcgca aggagagttc ctcacccgtg
     8641 aaagcgggcg gcggtgtctc tacctgatag atgattttgc ctctgagctt gatgatgagc
     8701 gtcgcgggct gcttgccagc cgcttaaaag ctacgcaatc gcaggtcttt gtcagcgcga
     8761 tcagtgctga acacgttata gacatgtcgg acgaaaattc gaagatgttt accgtggaaa
     8821 agggtaaaat aacggattaa cccaagtata aatgagcgag aaacgttgat gtcgaattct
     8881 tatgactcct ccagtatcaa agtcctgaaa gggctggatg cggtgcgtaa gcgcccgggt
     8941 atgtatatcg gcgacacgga tgacggcacc ggtctgcacc acatggtatt cgaggtggta
     9001 gataacgcta tcgacgaagc gctcgcgggt cactgtaaag aaattatcgt caccattcac
     9061 gccgataact ctgtctctgt acaggatgac gggcgcggca ttccgaccgg tattcacccg
     9121 gaagagggcg tatcggcggc ggaagtgatc atgaccgttc tgcacgcagg cggtaaattt
     9181 gacgataact cctataaagt gtccggcggt ctgcacggcg ttggtgtttc ggtagtaaac
     9241 gccctgtcgc aaaaactgga gctggttatt cagcgcgagg gtaaaattca ccgtcagatc
     9301 tacgaacacg gtgtaccgca ggcaccgctg gcggttaccg gcgagactga aaaaaccggc
     9361 accatggtgc gtttctggcc cagcctcgaa accttcacca atgtgaccga gttcgaatat
     9421 gaaattctgg cgaaacgtct gcgtgagttg tcgttcctca actccggcgt ttccattcgt
     9481 ctgcgcgaca agcgtgacgg caaagaagac cacttccact atgaaggcgg catcaaagcg
     9541 ttcgttgaat atctgaacaa gaacaaaacg ccgatccacc caaatatctt ctacttctcc
     9601 accgaaaaag acggtatcgg cgttgaagtg gcgctgcagt ggaacgatgg cttccaggaa
     9661 aacatctact gctttaccaa caacattccg cagcgtgacg gcggtactca cctggcaggc
     9721 ttccgtgcgg cgatgacccg taccctgaat gcctacatgg acaaagaagg ctacagcaaa
     9781 aaagccaaag tcagcgccac cggtgacgat gcgcgtgaag gcctgattgc ggtcgtttcc
     9841 gtaaaagtgc cggacccgaa attctcctcc cagaccaaag acaaactggt ttcttctgag
     9901 gtgaaatcgg cggttgaaca gcagatgaac gaactgttgg cggaatacct gctggaaaac
     9961 ccaactgacg cgaaaatcgt ggttggcaaa attatcgatg ctgcccgtgc ccgtgaagcg
    10021 gcgcgtcgcg cgcgtgaaat gacccgccgt aaaggtgcgc tcgacttagc gggcctgccg
    10081 ggcaaactgg cagactgcca ggaacgcgat ccggcgcttt ccgaactgta ccttgtggaa
    10141 ggggactccg cgggcggctc tgcgaagcag gggcgtaacc gcaagaacca ggcgattctg
    10201 ccgctgaagg gtaaaatcct caacgtcgag aaagcgcgct tcgataagat gctctcttct
    10261 caggaagtgg cgacgcttat caccgcgctt ggttgtggta tcggtcgtga cgagtacaac
    10321 ccggacaaac tgcgttatca cagcatcatc atcatgaccg atgcggacgt cgacggctcg
    10381 cacattcgta cgctgctgtt gaccttcttc tatcgtcaga tgccggaaat tgttgaacgc
    10441 ggtcacgtct acatcgctca gccgccgctg tacaaagtga agaaaggcaa gcaggaacag
    10501 tacattaaag acgacgaagc gatggatcag taccagatct ctatcgcgct ggacggcgca
    10561 acgctgcaca ccaacgccag cgcaccggca ttggctggcg aagcgttaga gaaactggtg
    10621 tctgagtaca acgcgacgca gaaaatgatc aaccgcatgg agcgtcgtta tccgaaagca
    10681 atgctgaaag agcttatcta tcagccgacg ctgacggaag ccgacctctc tgatgagcag
    10741 accgttaccc ggtgggtgaa cgcgctggtc agcgaactga acgacaaaga acagcacggc
    10801 agccagtgga agtttgatgt ccacaccaat gccgaacaaa acctgttcga gccgattgtt
    10861 cgcgtgcgta cccacggtgt ggatactgac tatccgctgg atcacgagtt tatcactggt
    10921 ggcgaatatc gtcgtatctg cacgctgggt gagaaactgc gtggcttgct ggaagaagat
    10981 gcgtttatcg aacgtggcga gcgtcgtcag ccggtagcca gcttcgagca ggcgctggac
    11041 tggctggtga aagagtcccg tcgcggcctc tccatccagc gttataaagg tctgggcgag
    11101 atgaacccgg aacagctgtg ggaaaccacc atggacccgg aaagccgtcg tatgctgcgc
    11161 gttaccgtta aggatgcgat tgccgctgac cagttgttca ccacgctgat gggcgacgcc
    11221 gttgaaccgc gccgtgcgtt tattgaagag aacgccctga aagcggcgaa tattgatatt
    11281 taatggcgtt aacaatgcga gcgtgcctga tgcgctacgc ttatcaggcc tacgagactc
    11341 ctgcaatata ttgaatttgc atgtttttgt aggccggata aggcgttcac gccgcatccg
    11401 gcaagaagct ataagaaaag ggcggagatc atctccgccc tttttatttc tgcaatccgc
    11461 aattatcatc ccgcagttct atactttttg ggtcaacacg taacgcttta caggtgatta
    11521 tcatggggct ttttgatgaa gttgtcggcg cctttctgaa aggcgatgcg gggaaatatc
    11581 aggcgatttt aagttgggtt gaggagcagg gtggcattca ggtgctgctg gaaaaactgc
    11641 aaagtggcgg cttaggggcc attctctcaa cctggctgag taatcaacag ggcaatcaac
    11701 cagttagcgg cgagcaactg gaatcagcac tcggcacaaa cgcggtatcc gatcttggac
    11761 aaaaacttgg cgtggatacc agtacggctt cctgtttact ggcagaacaa ttgccgaaga
    11821 ttattgatgc gctctcaccg caaggtgaag tgtcaccaca agccaataac gatctgcttt
    11881 ccgcaggcat ggaactgctg aaagggaaac tcttccgcta agcaaatggg gagcatgcaa
    11941 cccgcgttgc atcccccttt tccctcgggt gttgtactga tttttgagcg gaatcgcgtt
    12001 agcatgggtc aggaaccaat ctacctgggg aactcatggc tatcaaactc attgctatcg
    12061 atatggatgg cacccttctg ctgcccgatc acaccatttc acctgccgtt aaaaatgcga
    12121 ttgccgcagc tcgcgcccgt ggcgtgaatg tcgtgctaac gacgggtcgc ccttatgcag
    12181 gtgttcacaa ttacctgaaa gagctgcata tggaacagcc gggcgactac tgcattactt
    12241 ataacggcgc gctggtacag aaggccgctg atggtaacac cgtggcgcaa actgctctca
    12301 gctatgacga ctatcgtttc ctggaaaaac tctctcgcga agtcggttct catttccacg
    12361 ccctggaccg caccacgctg tacaccgcca accgtgatat cagctactac acggtgcatg
    12421 aatccttcgt tgccaccatt ccgctggtgt tctgcgaagc ggagaaaatg gaccccaata
    12481 cccagttcct gaaagtgatg atgattgatg aacccgccat cctcgaccag gctatcgcgc
    12541 gtattccgca ggaagtgaaa gagaaatata ccgtgctgaa aagtgcgccg tacttcctcg
    12601 aaatccttga taaacgcgtt aacaaaggta cgggtgtgaa atcactggcc gacgtgttag
    12661 gtattaaacc ggaagaaatc atggcgattg gcgatcagga aaacgacatc gcaatgattg
    12721 aatatgcagg cgtcggtgtg gcaatggata atgctattcc ttcggtgaaa gaagtggcga
    12781 actttgtcac caaatccaac cttgaagatg gcgtggcgtt tgctattgag aagtatgtgc
    12841 tgaattaatc tgtgggcggg caaacagcat aaacgcgttt gcccgcttac tgattattgg
    12901 tttactgcat atacaaccag aaattgcagc ggcgcagtaa tgatgtccag cgtaacagtc
    12961 actggcagta atgctgcgga aacaccatcc ggaatgcctc tgggtgaata gtattcataa
    13021 aatcgcactt taaatggatg gtagaacgcc atcacctttg agtaatccat gtttttattt
    13081 ttctcatgga ttgtgccttt taggttttcc agtgacctgg tacagtgttg aacaccacag
    13141 gcaaaaccat aatgcgttgc cagtgccttt tcttcttcgt tattccagta gtagtaaagc
    13201 gatattgtgc cggtaaattt ctttttcccc gcatttaaaa caaatcttgc gtcattggca
    13261 acctgaatat tgtgccggtt aagtgctgga tcattgagca tcttaacgat gtcatcggca
    13321 ccatcagtga taaggtaatc cagcgattcc cctacaaaaa catagccttt tgttccatta
    13381 ctatcttttg cctgcgatag cccagtgatg gtgtctgact gcgattgata ctttcctgaa
    13441 ctaccctttg cggtatggga aaccagagca tacgagatac agcctgaaag catcagcgcc
    13501 agtgggaata aactggcagc cttaaaaaat cccttaaaat tcaacttcat gattaatcct
    13561 ttaaacgagt agctaagccg ttgaaagcat actcttcttt ccctcctgtt tagcgcctga
    13621 tctccctttt cagatttctg cccgatataa gaccttttct tatgaggtgt ttttttgtgg
    13681 actaatatct gttgcaaggc tatgggttcg cacctgcact ggtttatatc tccgccgtcg
    13741 cgttgattgg cgcgctctct tacatcctgc tggtgggcga tgtgacgcgc gttggctaat
    13801 ctttcaactg tggaatatgc gccataccac tgcgcataat ccactttctt aaagccggtg
    13861 cggtcaggca tcggcttttt actatcttgc cgaagagttt taaacgcagt ttgcgggtgg
    13921 cgtgccagta ggctgcgttg atcttctctg gctgcttcag taacaccgaa cgcagaatct
    13981 ctgcgctatc cagcgcatag cttatcccct ccagcgagct ggcgctgata aatcccgccg
    14041 cttcaccaat cagaaaggcg ttgtccttac cgcagacaaa atcctgccag cgtgagggaa
    14101 atagcaccgt gcatttttcg ctttttaccg ccttaccaaa ctggaattga aaggcgctca
    14161 ttttctcttt cagcgtcgtg aaacgcgtct gaccgtcttt cattggatag gcaccgccaa
    14221 agataaaata accgtctttg ctgatactcc atgagtaaca gtcagttatc gcattatcaa
    14281 agatgcatga gtagaacggc accggatgtt tctctgcgaa ccactgctgg atagcgacat
    14341 atttacgaat ctgatggtcc gggtagagat atcgccgcac catcgagttg gcaccatctg
    14401 caccgaccag atagcgggca gtaatatgct gctcccaacc gtctgcacga aaaatgacat
    14461 gccatttatc atcctcacgc cagattttcc ggcacaggct atcgtgataa acctcaacgc
    14521 tggcggggat cagcgatttc atccacaagt cgaaagcatg gcgattaata ttgatatagc
    14581 ttcgctggta gttacgcgtg agcgatgcgg cgacgtcgac agttttgacg ctgaaaatct
    14641 gtggattggc gatcacatcg acaggaagcg tcagtccatc gcgaataaaa gatcgctgcg
    14701 cgtccggtgc cagcagaccg ccacaaggct tgctgaaacc ttcagtacca tgctggtgct
    14761 ttttatccag cgcgatcacc tgcattttgc cggctaactt tcgtgccaac gccgaccctg
    14821 ccgggccgag gccaataatc gccacgtcga aatgttccat cttttcactc acattcatca
    14881 cgatggcaaa agactattgt ctgaaagtga agtccttgtg aagtatgatg gcgggaaccg
    14941 ttttgtatca ggaccgcgcc atgaaacaga taacctttgc tccccgtaat cacctgctca
    15001 ccaataccaa tacctggacg cccgacagcc agtggctggt atttgacgtg cgtccttctg
    15061 gcgcgtcgtt taccggcgag accattgagc gtgtgaatat ccataccggc gaggtcgagg
    15121 ttatctatcg cgtgtcacag ggcgcgcacg tcggcgtggt gaccgttcat ccaaagtcag
    15181 agaaatatgt ctttatccac ggacctgaaa atcctcatga aacatggcat tacgatttcc
    15241 atcaccgtcg cggcgtgatt gttgaaggcg gcaagatgaa caatctcgat gcaatggata
    15301 ttaccgttcc gtacacatca ggggcgctgc gtggcggcag ccatgtgcat gtctttagcc
    15361 cgaacggtga aatggtgagt tttacctata acgaccatgt aatgcatcag ttcgattcgg
    15421 cgctggattt gcgaaacgtc ggggttgctg caccgtttgg cccggtcaac gtacaaaagc
    15481 agcatccgcg tgaatacagc ggtagccact ggtgcgtgct ggtgagtaaa accacgccca
    15541 cgccgcagcc tggcagcgat gaaatcaatc gtgcttatga agaaggatgg gtaggaaatc
    15601 acgcgctggc gtttattggc gacacacttt cgccaaaggg cgagaaagtg ccggagctgt
    15661 ttatcgttga gttaccgcaa gatgaagctg gctggaaagc agcaggtgat gcgccgttaa
    15721 gtggaacgga aacaaccctg cccgcgccac cgcgtggcat cgtgcagcga cgtttaacct
    15781 ttacccacca tcgggcttat ccggggttag tcaacgtccc gcgccactgg gtgcgctgta
    15841 atccgcaggg tacgcaaatc gcgtttttaa tgcgagatga taacggcatt gtgcaactgt
    15901 ggcttatctc gccacagggc ggcgagccgc gccagttaac ccataacaaa acggatattc
    15961 agtctgcatt taactggcat ccgtcaggag aatggttggg ctttgtgctg ggtaatcgaa
    16021 ttgcttgtgc ccatgcgcaa agcggcgagg ttgagtattt aaccgaaaac cacgccaatc
    16081 caccttctgc ggacgccgtg gtcttctcgc cggatggtca gtggctggcg tggatggaag
    16141 gcggccagct gtggatcacc gaaactgatc gctaaatcag ttatcaggca ttctggccgg
    16201 tggaataacc gcattggtgg cttgcgcgtt ggcttcgctt ttttcaacgc gtgagcgcac
    16261 cgagctgtct ttgcggaaca catcccacgg taataaaagc gtgtccatca cagcggtaaa
    16321 cggcatatcg agaatcgcca gcgacttagt gccccagttg gtttcatcat cgccaatcat
    16381 ggtggcgctg gcgcgggtgc cgggatacgt tccttctttc cctccggtat gggacatcac
    16441 gctggagcat cctccgagta atgccattcc gctgcatatc atgaacgcta acaacacatt
    16501 tcttatcata ataaattcat ctgttgatcg tgggtgttgg cctgatgagt tatagcgatc
    16561 ccttgctgaa aataacatca tcattacgtc gctctgtggc ggctatcgca ctttaacgtt
    16621 tcgtgctgcc ccctcagtct atgcaataga ccataaactg caaaaaaaag tccgctgata
    16681 aggcttgaaa agttcatttc cagacccatt tttacatcgt agccgatgag gacgcgcctg
    16741 atgggtgttc tggctacctg acctgtccat tgtggaaggt cttacattct cgctgatttc
    16801 aggagctatt gattatgcgt aactttgatt tatccccgct ttaccgttct gctattggat
    16861 ttgaccgttt gtttaaccac ttagaaaaca accagagcca gagtaatggc ggctaccctc
    16921 cgtataacgt tgaactggta gacgaaaacc attaccgcat tgctatcgct gtggctggtt
    16981 ttgctgagag cgaactggaa attaccgccc aggataatct gctggtggtg aaaggtgctc
    17041 acgccgacga acaaaaagag cgcacctatc tgtaccaggg catcgctgaa cgcaactttg
    17101 aacgcaaatt ccagttagct gagaacattc atgttcgtgg tgctaaccta gtaaatggtt
    17161 tgctgtatat cgatctcgaa cgcgtgattc cggaagcgaa aaaaccgcgc cgtatcgaaa
    17221 tcaactaatt tcctaaggcc gccttgcgcg gcctgacatc tccatgctcg ccgtcaggga
    17281 gcatatgcga atcttcggat ttgcaggtac ttactcgctt cttagaagga gaaatgacta
    17341 tgcgtaactt cgatttatcc ccactgatgc gtcaatggat cggttttgac aaactggcca
    17401 acgcactgca aaacgccggt gaaagtcaga gcttcccgcc gtacaacatt gagaaaagcg
    17461 acgataacca ctaccgcatt acccttgcgc tggcaggttt ccgtcaggaa gatttagaga
    17521 ttcaactgga aggtacgcgc ctgagcgtaa aaggcacgcc ggagcagcca aaagaagaga
    17581 aaaaatggct gcatcaaggg cttatgaatc agccatttag cctgagcttt acgctggctg
    17641 aaaatatgga agtctctggc gcaaccttcg taaacggttt actgcatatt gatttaattc
    17701 gtaatgagcc tgaacccatc gcagcgcagc gtatcgctat cagcgaacgt cccgcgttaa
    17761 atagctaact agctattctc tttgccccgc cattctggtg gggctttttt tgtctttctt
    17821 cttcaacaat gcgttaaatt tcgactgttt aagatatttc ggcacgttct ttcccgtgat
    17881 aatgtgcgct ataaggcaaa tctgcttcac cgattcagcc ataatccgta gcaattaaaa
    17941 tgttatctca agagaaatgg acgatgagtg atatagcatt aacggtcagt attttggctt
    18001 tggtggcagt cgtcggtttg tttatcggca acgtcaaatt tcgcggcata ggattaggta
    18061 ttggcggcgt gctgtttggt gggatcatcg tcggccattt tgtttctcag gcggggatga
    18121 cattaagtag cgatatgctg catgttattc aggaatttgg cctgatcctg ttcgtttata
    18181 ccatcgggat tcaggtaggg ccgggcttct ttgcctcatt gcgcgtctcc ggattacgcc
    18241 tcaacctgtt tgctgttctg atcgtcatca tcggtggtct ggttaccgcc atcctgcata
    18301 aactgtttga tattccactg ccggtagtgc tggggatttt ctccggtgcg gtaaccaata
    18361 cgccagcgct gggggcaggg cagcagattt tgcgcgacct gggtacacca atggaaatgg
    18421 tcgatcagat ggggatgagt tacgcgatgg cgtatccatt cggcatttgc gggatattgt
    18481 tcaccatgtg gatgttgcgg gttattttcc gcgtcaatgt cgagacagaa gcccagcagc
    18541 acgagtcttc acgcaccaat ggcggcgcgc tgatcaagac tatcaatatt cgcgttgaga
    18601 accctaacct gcatgattta gccattaaag atgtaccgat tctcaacggc gacaaaatta
    18661 tctgctcgcg tctgaaacgc gaagaaaccc taaaagttcc ttcgccagat accattatcc
    18721 aactgggcga tttgctgcat ctggtgggtc agccagcgga tttacataat gcgcaactgg
    18781 tgattggtca ggaggtcgat acttcgctgt ccacgaaagg cactgatttg cgcgtcgagc
    18841 gtgtggtggt caccaatgaa aacgtgcttg gtaagcgtat tcgcgacctg cattttaaag
    18901 aacgctatga cgttgttatc tcgcgcctga accgtgccgg ggtcgaactg gtcgccagtg
    18961 gcgatatcag cctgcagttc ggcgatatcc tcaacctggt ggggcgtccg tccgcaattg
    19021 atgccgttgc caatgtgctg gggaatgcgc agcaaaaact gcaacaggtt cagatgctgc
    19081 cagtgtttat tggcatcggg cttggcgtat tgttaggttc tattcccgtc tttgtgccag
    19141 gattcccggc cgcgttgaaa ctggggctgg cgggcggtcc gctgattatg gcgttgatcc
    19201 tcgggcgtat cggcagtatc ggcaagctgt actggtttat gccgccaagt gccaacctcg
    19261 cgctgcggga actggggatc gtgctgttcc tctcggtcgt tggtctgaaa tctggtgggg
    19321 attttgtgaa taccctggtc aatggcgaag ggctaagctg gattggttat ggtgccctga
    19381 tcaccgccgt tccgctgatt actgttggca ttctggcgcg gatgttagcc aaaatgaatt
    19441 acctgaccat gtgcgggatg ctggcaggtt ccatgaccga tcctccggcg ctggcgtttg
    19501 ctaataatct tcatccaacc agcggtgcgg cggcgctctc ttacgccact gtctatccgt
    19561 tagtgatgtt cctgcgcatt atcacccccc aattactggc ggtgctcttc tggagtatcg
    19621 gttaactctt gtccgggtgg agtcgccgta aatggtactc cacctggtat tcgctggcat
    19681 ttctgaacat cactgaataa ttcaaaaact ccccgctctc gctatatgac aacgaggtga
    19741 tccgcagcag tggcgtgtgt tccgctacct tcatttgccg cgccagcagt ctatcggcga
    19801 gcaccggcgt caggctttca taattaccgc caatcaaaat cccgcactct tgttcaatat
    19861 attcaaactt cgacccttcc agatgttgca gcgaaaggtt acggaacagt tttaccggca
    19921 tatagctgtc ttccagcatc agcggtttcc cttccacaaa acgaacgcga cgggagaagt
    19981 agatctgctc gttgatttga atccgcaact ggctggcaat tgccggaggc gcaggcatga
    20041 tttcaaaaat cagcacctgg ctggtgactg tttttccctg ccgttttaac acctccacca
    20101 gcccggtcag gctggcggtt tgatgcagca catctttgcg caccaggtaa gtgccgctgc
    20161 cgtggcggcg gaccaccagc ccccacgcta ccagcaggtc aatcgcttta cggatggtca
    20221 tccgcgatac cgcaaactct tccgccagct ttttttcacc gggaagaagg ctgtttagcg
    20281 tgaaatccgc ggaattaagt cgaattctta accgctccgc aatgcttttg tagatcatct
    20341 ctagatctct tttttgcctg caagcccggg taatgcgccc acaagttctg cttaatcaat
    20401 tgaaaaaaca tctttttttt aaagatgtgt tcgatgctgt gtaccagtgc tcacagagat
    20461 ctactttttc gcgaaaaagt agatctctac cgcccaacga aaagcatgaa agcgatcacg
    20521 aatcccattg ggtcgtcatg ttctgccgat cgcatcttcc tatcctcgct ccaggcctgc
    20581 cgcataacca atcaggcttc ctacttacag aattgagaaa agaggatgtg gaaatgctca
    20641 gtcaaattca acgctttggc ggcgcgatgt tcacgccagt gctgctgttt cccttcgccg
    20701 ggattgtggt gggtcttgcc atcttgctgc aaaacccgat gtttgtcggg gaatcactga
    20761 ccgatccgaa cagtttattc gcgcaaatcg tacacattat tgaagagggc ggttggacgg
    20821 tattccgtaa tatgccgctg atttttgctg tcggtttacc cattggcctt gctaagcaag
    20881 cgcaggggcg tgcttgtctg gcggtgatgg tgagtttcct gacctggaac tatttcatca
    20941 atgcgatggg aatgacctgg ggaagctact tcggcgtcga tttcactcag gacgcggtgg
    21001 caggtagcgg tctgacaatg atggccggga ttaaaaccct cgataccagc attatcggcg
    21061 caattatcat ttccggcatt gtgacggcgc tgcataaccg tctgttcgat aaaaaactgc
    21121 cggtttttct cggcattttc caggggacgt cttatgtggt gattatcgcc ttcctggtga
    21181 tgatcccctg tgcctggctg acattgctcg gctggccaaa agtacaaatg gggattgaat
    21241 ctctgcaagc gttcctgcgt tcggcgggtg cgcttggggt gtgggtttac accttcctcg
    21301 aacgtattct gatcccaacc ggtttacacc acttcatcta cggaccgttt atctttggtc
    21361 cggcagctgt tgaaggcgga attcagatgt actgggcgca gcatctgcaa gagttcagtt
    21421 tgagcgccga gccgctgaaa tcgttgttcc cggaaggagg ttttgccctg cacggtaact
    21481 caaaaatctt tggtgccgtg ggcatttctt tagcgatgta cttcactgcc gcaccggaaa
    21541 atcgggtaaa agtggcgggt ttgctgattc ccgcaacctt aaccgccatg ctggtgggaa
    21601 ttaccgaacc gctggaattt accttcctgt tcatttcacc gttgctgttt gcggtacacg
    21661 ctgtgcttgc ggcctcaatg tcgaccgtga tgtatctctt tggtgtggtg ggcaacatgg
    21721 gcggaggtct gattgaccag gttttaccgc aaaactggat cccgatgttc agcaaccacg
    21781 cggatatgat gctgacccaa atcgccattg ggttgtgctt taccctgctg tacttcgtgg
    21841 ttttccgcac cctgattctg caattcaaca tgtgcacgcc gggacgtgaa gatgcggaag
    21901 tgaaactcta ctcaaaagcc gaatacaaag cctcgcgagg ccaaaccacc gcggcagagc
    21961 caaaaaaaga gctggatcag gctgccggta tcctgcaagc cctgggcggg gtcggcaata
    22021 tctccagcat taacaattgt gcgacgcgtc tacgcattgc actgcatgac atgtcacaaa
    22081 cgctggatga cgaagtcttt aaaaagctgg gagcgcacgg cgtcttccgt agtggcgatg
    22141 ccattcaggt gatcattggt ctgcatgtat cccagctgcg tgaacagctc gatagcttaa
    22201 ttaattctca tcaatcagca gaaaatgttg ccattacgga ggcagtataa tgaccaaatt
    22261 ctcagtggtt gtcgcaggcg gtggaagcac ctttacgcca ggcatcgtgt tgatgctcct
    22321 ggcgaatcag gaccgtttcc cgcttcgtgc actgaaattt tatgataacg atggttcgcg
    22381 gcaggaagtg attgccgaag cctgtaaagt catccttaaa gaaaaagcgc cggatattgc
    22441 gtttagttac accaccgatc ctgaagtggc gttcagcgac gttgattttg tcatggcgca
    22501 catccgcgtc ggcaaatacc cgatgcgcga actggatgaa aaaatcccgc tgcgccacgg
    22561 cgttgttggt caggaaactt gcggacccgg cggaatagcg tacggcatgc gttccattgg
    22621 cggcgtcctg gaactggtgg attatatgga aaaatattca ccaaacgcct ggatgctcaa
    22681 ctactccaac ccggcagcaa ttgttgcaga agccacgcgt cgtctgcgcc cgaatgcgaa
    22741 aatcctcaac atctgtgaca tgccaatcgg tattgaaagc cggatggcgc aaattgttgg
    22801 gctgcaagat cgcaaacaga tgcgcgtgcg ctactacggc ctgaaccact ttggctggtg
    22861 gacatcaatt gaagatttgc agggcaacga cctgatgcac cagctgcggc aatatgtctc
    22921 taagcacggt tatgttccac cgcagcaaga tacacatact gaagcgagct ggaacgacac
    22981 ctatgcaaaa gcgcgggatg tccaggcact ggacccggat acattgccga acacctatct
    23041 gaaatattat ctcttcccgg attatgtcgt tcagcattcc aaccctgaac atactcgcgc
    23101 gaatgaggta atggaacatc gcgagaaaca ggttttcgat gcttgccgcg ccattacggc
    23161 ggcaggaaat tcagcggcgg gcaagctgga aattgacgaa catgcgtcat acatcgtcga
    23221 tctggcggcg gcaattgcct tcaacactca ggagcggatg ttgctgattg tgcctaacaa
    23281 cggggcaatc cataactttg atgatgaagc tatggtcgag atcccgtgtc tggtcgggca
    23341 caacggacca gaaccactgg tggtcggcga tatcccgcag tttcagaaag ggttaatgag
    23401 tcagcaagtg gcggtggaaa aactggtcgt ggacgcctgg gaacagcgtt catatcagca
    23461 cctgtggcag gcgattacgt tgtcgaaaac ggtaccgagc gcctcggtcg ccaaagctat
    23521 tctggatgag ttgctggagg ccaacaaagc gtactggcca gagttacgtt aattgtgtgg
    23581 actggcatcc gttgggtgct ggtccgtatt ctttagcacg gctgaaggcg cataaccaaa
    23641 tttatgttta aaggctttgc tgaaatgaaa ggagtcgaag aagttgagcg tatcagcgac
    23701 ttcgccaacc gagtttcctt gttgctttaa tagcgatagc gccagatcca gacgcgcatc
    23761 gaggtaatat tctttcggcg tctttccggt atagcgtaaa aacagacgac gcaaccaggc
    23821 ttcgctacag gggatcgtgg cagccatatc ggctacgctc cagcgttgtt gcagactggc
    23881 atgtaaagtg gcaattaatt tttcaatttg ccgccgttgt ggatcttttt taccgtctgc
    23941 gtacataaga caaatccact gataaataat tttcgtcaga aaagcgactg ccagattatt
    24001 ttttattgct tctggtgaag ttattaactc agcaacttcg gtgagttcct gattataaat
    24061 ttcgccattg taaataacgc tttgctgacc gatgggaata tccatcatac tggtgggggt
    24121 aaattccatc cagtattgtt cccagactaa accttcacag tgataagagt gaatatccat
    24181 tggctttaaa aatataatac agttgccatg cagtgttatt tgttcgccat tctttaaaag
    24241 aatcttccca catccttgta cggtatacac cgcaacccat gagttggcga taagcggttt
    24301 cttcttgtcc cgtggccaga tcacccggta atgctcgtcg gcgtaggtat gccagagcga
    24361 gatcagagaa attccgctgg agatcacatt ttcgttaatc aataaaggaa tattgtatgg
    24421 agtttcgttt tttacatctg aagtttgcaa ttttccattc attttaatat ccctccggcg
    24481 aattatgctc acaacctgaa cttaacagaa aacaatccgt tcagggatac gacgtttgta
    24541 ttttaagaat ctgactgcct gacccgacgc attttacctc tccctatatt catgcgtccg
    24601 ggacataaac ataaataagg gctatgagat gaattcgtta caaatcttga gttttgtcgg
    24661 ttttacgctg ctggtggcga tcatcacctg gtggaaggtt cgcaaaacag ataccggatc
    24721 gcaacaaggc tattttcttg ccggacgttc actaaaagcg ccggttattg ccgcttcgtt
    24781 aatgttaacc aacctttcca cggaacaact ggttggtctt tccgggcagg cctacaaaag
    24841 cggcatgtcg gtgatgggct gggaagtgac ttcagcggtg acgctgatct tcctcgcgct
    24901 aatcttttta ccgcgctatc tgaagcgcgg cattgccacc atccccgatt ttctggagga
    24961 acgttatgat aaaacgacgc gtatgatcat cgacttctgc ttcctcattg ccaccggcgt
    25021 ctgctttctg ccgattgttc tctactccgg cgcgttggcg ctcaacagcc tgtttcacgt
    25081 cggggaatcg ctacagattt ctcacggtgc ggctatctgg ctactggtaa ttttgcttgg
    25141 tctggcggga attttgtatg cggtgatcgg cggactgcgc gcaatggcag tggcggactc
    25201 catcaacggt attgggctgg ttatcggcgg gttgatggtg ccggtatttg gcttgatcgc
    25261 gatgggcaag ggcagcttta tgcagggcat tgagcaaatt accaccgttc acgccgagaa
    25321 attaaactca gtcggtggcc cgaccgatcc cttgccgatt ggcgcggcat ttaccggttt
    25381 aattctggtg aacacctttt actggtgtac aaatcagggc atcgtgcaac gcacgctggc
    25441 gtcaaaaagc ctggcggaag ggcaaaaggg ggcgctgtta acggcggtgc tgaaaatgct
    25501 cgacccgctg gtactggtgc tgccagggtt gattgcgttt catctgtatc aggatctacc
    25561 gaaagccgac atggcctacc cgacgctggt caataacgtt ctgccagtgc cactggtggg
    25621 tttcttcggc gcggtgttat ttggtgcagt gatcagtacc ttcaacggct ttctgaatag
    25681 cgccagtacg ttattcagta tgggtattta ccgtcgcatc attaaccaga atgccgagcc
    25741 gcagcagctg gtcaccgtcg ggcgcaaatt tggtttcttt atcgctatcg tttcggtgct
    25801 ggtagcgccg tggatcgcca acgcgccgca ggggctgtat agctggatga aacagctcaa
    25861 cggcatttac aacgtgccgc tggttaccat catcattatg ggctttttct tcctgcgcat
    25921 cccggcgctg gcggcaaaag tggcgatggg gattggcata atcagctaca tcaccatcaa
    25981 ctatctggtg aagttcgact tccatttcct ctatgtgctg gcctgtacgt tctgcatcaa
    26041 cgtggtcgtg atgctggtga tcggttttat caaaccgcgc gccacgccgt tcaccttcaa
    26101 agatgcgttt gcggtggaca tgaaaccgtg gaaaaacgtc aagatcgcgt caattggcat
    26161 cctgttcgcg atgattggcg tctatgccgg gctggctgaa ttcggcggct acggtacgcg
    26221 ctggttagcg atgatcagtt atttcattgc cgccgtagtg attgtctacc tgatttttga
    26281 cagctggcgg catcgtcacg acccagccgt aacctttact cccgacgcga aggatagcct
    26341 atgaaacgcc ccaattttct gttcatcatg accgataccc aggcccccaa tatggtcggt
    26401 tgctatagcg gtaaaccgct gaatacgcaa aatattgata gtctggcggc ggaaggtatt
    26461 cgctttaatt ccgcctacac ctgttcaccg gtttgtacac cggcgcgcgc cgggctgttt
    26521 accggtatct acgctaacca gtccgggccg tggaccaaca acgtcgcgcc gggtaaaaac
    26581 atctccacta tggggcgcta ctttaaggat gccggctatc acacctgtta catcggcaaa
    26641 tggcatctcg acggtcatga ctatttcggc actggcgagt gtccgccgga gtgggacgct
    26701 gattactggt tcgatggggc gaactatctt agcgaactga cggaaaaaga gattagcctg
    26761 tggcgcaatg gcctaaacag cgtcgaagat ttacaggcga accatattga cgaaaccttc
    26821 acctgggcgc accgcatcag caatcgagcg gtggattttc tgcaacagcc cgcgcgcgcc
    26881 gaggaaccct tcctgatggt ggtttcgtat gatgagccgc atcacccgtt cacctgtccg
    26941 gtggagtatt tagagaaata cgctgatttt tactacgagc tgggcgagaa agcacaggat
    27001 gacctggcga acaaaccaga acatcaccgc ttatgggcgc aggcgatgcc atcgccagtc
    27061 ggtgatgacg ggctttatca ccatccgctc tattttgcct gtaatgactt tgttgatgac
    27121 caaatcggac gggtcatcaa tgccttaaag ccagagcaac gtgaaaatac gtgggttatt
    27181 tatacctccg atcacggcga aatgatgggc gcacataagc tgatcagtaa aggggcggcg
    27241 atgtatgacg acatcacccg cattccgctg atcatccgtt cgccgcaagg ggagcggcga
    27301 caggtcgata cgccagtcag tcatatcgat ttactgccga caatgatggc gctggcagat
    27361 attgaaaaac cagagattct gccgggggaa aatatccttg cagtgaaaga gccacgcggc
    27421 gtgatggtgg aatttaaccg ctacgagatt gagcatgaca gctttggcgg ttttattccg
    27481 gtgcgttgct gggtgacgga tgactttaaa ctggtgctca acctcttcac cagtgatgaa
    27541 ctttacgatc gccgtaatga tccaaatgaa atgcataacc tgatcgatga tgcccatttt
    27601 gccgacgttc gcagcaaaat gcatgatgcc ttattggatt atatggacaa aattcgcgat
    27661 ccgttccgca gttaccaatg gagcctgcgt ccgtggcgta aagatgcaca gccgcgctgg
    27721 atgggggcat ttcgtccacg cccacaagat ggttattcgc cagtggtacg cgactatgac
    27781 accggcctgc cgacacaagg ggtgaaggtg gaagagaaaa aacagaagtt ctgacgataa
    27841 tgcccgctga tgaacacccg gcgggcatta ttcaggctgc ttaaaccagt gaaccgccag
    27901 tcaggaagga aaagataagg aatatcagca aaatgctgca cacgatgccg ataccaaaaa
    27961 gcaaggtatg gaaagccagc gtcccgccgc caaaaacccg cgcgccgcga atgccccagc
    28021 gattttcctt acgcataccg ccaataaatg cgcaaagtag cgcagcaatg gccagtgcga
    28081 taccgctttg atcgaggatt ttatcgacat ccacgtcctc ttttctctcc acggtggtaa
    28141 ttttttcgcc ttttattccg gcgagaagac ctcttttaat ttcagccgtt ttgtccgcca
    28201 cgatgctttc aagcgttggc ggtggtgcgg aaaaagggcc gaatgaaaag tggataatcc
    28261 ccatcattaa agcgatggca ccgaatagca tgcccaatga actgatttta ttttgcgcta
    28321 tgatacccat ctgacctcct tgtagcggat ggcaaaagca tatcaggaaa cgggcggacg
    28381 aaattcttaa aaaaaaccgc taaaggtaga gcgttagtga aattgcaaag gagcaacaat
    28441 gaaaatttcc cgcctcggag aagcgcctga ttaccgcttc tcgctggcta atgagcgtac
    28501 ttttctggcg tggatccgta ctgcactagg atttctggcg gcgggggttg ggcttgatca
    28561 acttgcgcca gatttcgcca cgcccgtcat tcgcgaactg ctggcgttgt tgttgtgtct
    28621 gttttccggc gggttagcga tgtatggcta cttgcgctgg ttacgcaatg aaaaagcgat
    28681 gcgtctgaaa gaggacctgc cctacaccaa cagcttatta atcatcagct taatattgat
    28741 ggtcgtagca gtgatcgtta tgggactggt gttgtatgcc ggatagccgc aaagccagac
    28801 gcattgccga cccagggctg cagccggagc gtacatcact ggcgtggttt cgtaccatgc
    28861 tgggctacgg cgcgttgatg gcgttggcta tcaaacacaa ctggcaccag gcgggcatgt
    28921 tattctggat ttcgattggc atcctcgcca tcgtggcgct gatcctctgg cactacactc
    28981 gtaatcgcaa tttaatggat gtcacgaata gcgatttttc ccaatttcac gtagtccgtg
    29041 acaaattttt gatctccctc gcggtgttat ctctcgcaat actgtttgct gtaacgcata
    29101 tacatcaact tatcgtattt attgagagag tcgcatgaca ggaagtcagg tcattgatgc
    29161 tgaagaggac aggcataaac tggttgtaga gtacaaggat gctctgcaac ctgccgattt
    29221 ttatcacaat ttcaaacaac ggggcatccg ctccgtacag cttattcccc atcttgaatt
    29281 tgatgaccgg ggcgatatga cggccgcttc ggtgacagca gagctgtggg ggaaattttt
    29341 aattgccctg tttgagtgtt gggtgcgggc agatatcagt cgtatctcga ttgagctttt
    29401 tgacgccaca ttacaaaaat ggtgtggcag cgaaaacccg caaccgcgcc gtggctgcca
    29461 ggcgtgcgac tggcatctgc tgtgtccgca cgctcgggag gagacgccag acagcgtgct
    29521 ttgtgcgggc tatcaggcgt tttattctta ctctgcgccg catatgcggg tgatgcgtga
    29581 tctgataaag cagcatcgtt ctcccatgga gctgatgacg atgctgcggt gacgtgagct
    29641 taaacgggct gcccctgatg cgacatccgc gtcgccagcg gtagccagca cagcacgatc
    29701 aacaatccca ttaaggtcat caacaacccc aggctgccct gaccggtttg cggcaacatc
    29761 gcagagagcg acgccagcac gccggaacca atgttttgta gaccgccgac cagcgcgcca
    29821 gccgtgcccg ccaggaaagg gaacggctcc atcgcgccgc tggtcgccag cggaaacagc
    29881 atcccggcac cgaaaaagaa cagcgcggcg ggaacgagca gcgtccagac attcatcacg
    29941 ccaaaccagt cggggatcca catcagtaag ccagccagca ggcagcagat aaccgactgc
    30001 cacatcaacg ttgagaagcg tttattagga cgtccggcaa accatgcccc aaaaaatgcc
    30061 gccggaatcg gcagaataaa caaaatactg accgtcatac tgctcagccc taacaccgcg
    30121 cccatcagca cgccggagca ggcttcaaag gcggcaatcc cggccagacc gccaatcagc
    30181 atcagcaaat aacagttaaa accgctgtta ccgaaaagcg ttttataact ggtaagcagg
    30241 cgcgtgcgcg gtgcgtcgac cggacgcgtt tccggcatcc agcgggccat actgaaggtc
    30301 acaccggcac aaagtaccaa caaaaagaga taacaggcgc gccagttcca catcgtatcc
    30361 agcagaccgc cgattagcgg tgcgagcaac ggactgacaa gaattcccat gtttaacagg
    30421 ctgttagcgt ggcgcaactg tgtccgttca tataaatcac gcggcaaagt acgcgccatt
    30481 acgccgccaa cgccggtgcc catcccctgc atcgcgctgg cggcaatcaa tactgtcaaa
    30541 ctggaggtcg tgaccgcgac cagcgttgcc agcataaaaa tggacattcc gacgaggatc
    30601 accggtcggc gacccacacg gtcggaaatc gggccataaa acagctgtga gacaccgtaa
    30661 gtcagcagat aagcgcccat tacgctctgc accgcccctt cacgaacgtt gagatcgcgc
    30721 gccatatcgg caatagctgg aatataaatg gtttgcgcca tctgaccgac ggccacgagt
    30781 aataccaaca tcaataacaa attgatgttt ctatgccttt tcattatcac ggatgctttt
    30841 ataaaaataa ttagaataag tcactacgca ttcccataaa tgcgggagag gaatctatca
    30901 tattgataaa tgttagccag atatacgcca ggaatgggga attgtttagc ggcagaatat
    30961 gtaaacaaaa gcggcaataa atgttgccgg gagggatggt aggtcggata agatgcgtca
    31021 gcatcgcatc cgacaccaac ccacacgcta aatataaaat gggagcggtt acccgctccc
    31081 ctttagtgcg acttgaatct gaattacttc aggtatttca gaacagcatc aagcagttgc
    31141 agtgctgcaa caatgagttt gaggataaga atggcgatat ccaccaggct catacgcgtc
    31201 tcctgtggtt caggagcact gccagctgac gtacctttcc gctgcctgtt gccagctgtt
    31261 gtgcacgcgg tatctcaagg agaaacgttg tgctcgttga cataacacag tgtgctcacg
    31321 cggggttaag gcgctgacgg caccacccgt ttcagccagg acttagtgcg ccgggtaacg
    31381 atgcggcccc cgcataacac attgcgtaca gtgatattat agaagcttgc tgtataaata
    31441 aacagtaata tttggacaaa acgcaaactg tgatcgatac tcaactatgc actaaatacg
    31501 tcaaaattcg tgccgaaatt gcgcgttttg cgcggaacac gtatactttc tgtgttgaca
    31561 taatacagtg tgctttgcgg ttaccagccg cgggcggctg acgaaacctc gctccggcgg
    31621 ggttttttgt tacctgcagt acagtacaaa acgtgatcaa cccctcaatt ttcccttgct
    31681 gaaaaatttt ccattgtctc ccctgtaaag ctgtgcttgt ataaatattg ttaaacacaa
    31741 aaccaacaag gtccccaatg gctacttcca tgctcaacgc aaaactacta ccaactgcgc
    31801 catccgccgc agtggtcgtc gtgcgtgtgg tggtggtcgt cggcaatgcg ccgtagggac
    31861 tggaacaaca cacgattcca aaaccccgcc ggcgcaaacc gggcggggtt tttcgtttaa
    31921 gcacctcccg gaaagtcggc ccagaagaaa aggactggag catggcaagt tcgggcacat
    31981 catcgacgcg taagcgcttt accggcgcag aatttatcgt tcatttcctg gaacagcagg
    32041 gcattaagat tgtgacgggc attccgggcg gttctatcct gcctgtttac gatgccttaa
    32101 gccaaagcac gcaaatccgc catattctgg ctcgccatga acagggcgcg gggtttatcg
    32161 ctcagggaat ggcgcgcacc gacggtaaac cggcggtctg tatggcctgt agcggaccgg
    32221 gggcgactaa cctggtgacc gccattgctg atgcgcggct ggactccatc ccgctgattt
    32281 gcatcactgg tcaggttccc gcctcaatga tcggcactga cgccttccag gaagtcgaca
    32341 cctacggcat ctctatcccc atcaccaaac acaactatct ggtcagacat atcgaagaac
    32401 tcccgcaggt catgagcgat gccttccgta ttgcgcaatc aggccgccct ggcccggtgt
    32461 ggatagacat tcctaaggat gtgcaaacgg ccgtttttga gattgaagca cagcccgcgg
    32521 tggcagaaaa agccgtcgcg cccgccttta gcgaagaaag cattcgtgac gcagcggcaa
    32581 tgattaatgc tgccaaacgc ccggtgcttt atctgggcgg tggtgtgatc aatgcgcctg
    32641 cgagagtgcg tgaactggcg gagaaagcgc agctgcctac cacaatgact ttaatggcgc
    32701 tgggcatgtt gccaaaagcg catccgttgt cgctgggtat gttggggatg cacggtgtgc
    32761 gcagcaccaa ctatattttg caggaggcgg atttgttgat tgtgctcggt gcgcgttttg
    32821 atgaccgggc gattggcaaa accgagcagt tctgcccgaa tgccaaaatt attcatgtcg
    32881 atatcgaccg cgcagagctg ggtaaaatca agcagccgca cgtagcgatt caggcggatg
    32941 ttgatgacgt gctggcgcag ctgatcccgc aggtggaagc gcaaccgcgt gcagagtggc
    33001 accagttggt agcggatttg cagcgcgaat tcccgtgtcc aatcccgaaa gcgtgcgatc
    33061 cgttaagcca ttacggcctg atcaacgccg ttgccgcctg tgtcgatgac aatgcgatta
    33121 tcaccaccga tgtgggtcag catcagatgt ggacggcgca ggcttatccg ctcaatcgcc
    33181 cacgccagtg gctgacctcc ggcgggctgg gcacgatggg ttttggcctg cctgcggcga
    33241 ttggcgcggc gctggcgaac ccggatcgca aagtgttgtg tttctccggc gatggcagcc
    33301 tgatgatgaa tattcaggag atggcgaccg ccagcgaaaa ccagctggat gttaaaatca
    33361 ttctgatgaa caacgaagcg ctggggctgg tgcatcagca acagagtctg ttctacaagc
    33421 agggcgtttt tgccgctacc tatccgggaa aaatcaactt tatgcagatt gccgccggat
    33481 tcggcctcga aacctgtgat ttgaataacg aagccgatcc gcaggctgca ttgcaggaaa
    33541 tcatcaatcg ccctggtccg gcgctgatcc atgtgcgcat tgatgccgaa gaaaaagtgt
    33601 acccgatggt gccgccaggt gcggcgaata cagaaatggt gggggaataa gccatgcaaa
    33661 acacaactca tgacaacgtg attctggaac tcaccgttcg caaccatccg ggcgtaatga
    33721 cccacgtttg tggccttttt gcccgccgcg cttttaacgt tgaaggcatt ctttgtctgc
    33781 cgattcagga cagcgacaaa agccatatct ggctactggt caatgacgac cagcgtctgg
    33841 agcagatgat aagccaaatc gataagctgg aagatgtggt gaaagtgcag cgtaatcagt
    33901 ccgatccgac gatgtttaac aagattgcag tgttttttca gtaaggagcg acattaaacc
    33961 gctcggggga gttactgatg agcgattttt gatagatccg tgcagatata tagggtgaaa
    34021 ttgtgccaga gaccatctca ggcacaattt attagcccag gacgttaatt taagtcgcag
    34081 caccggtaac ctggggggga cagttagggc taaaattttc agcgatggtg ttaaattctg
    34141 attgctgtct ataccagtgg tcatgaccag cattctatta attaatggtt catcgtgagg
    34201 tatattatgg caagtcctat aaacagcgga ataatgatgg caaatctctg cccttccacc
    34261 tttttggata aaaatagaaa tgtcgctgct gagcttgata ttaaaaataa cgagaaaaaa
    34321 tattcacctg gcagtaattt cgcaaaatgg atgttgcagg aaataaagcg cttaatacta
    34381 aacatcatgt ccggttctcg gagtattaac accgatattc ttgactattt ccaccctatg
    34441 ccaggcacgg aaaataatgg taatcgtacc tgggtggcgg caactggaga ggatgaatat
    34501 atcgaaatca aacaaacagg tgataagtct tttaatatta cactggttgg cagagataaa
    34561 ccttcacgaa aagaaattcc atattcagga gtggctgtag cgactattat taaatcgttg
    34621 tctgaaaaaa cttctgcgtt agaaacacat tccgctgaca cggttttgag gaagaaatta
    34681 gttaatagta ttgttatgaa gaatacggat tttaatt
//
LOCUS       DAZKFZ010000027        34639 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000027 DAZKFZ010000000
VERSION     DAZKFZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            209..361
                     /gene="mokC"
                     /locus_tag="ACHOQW_001249"
     CDS             209..361
                     /gene="mokC"
                     /locus_tag="ACHOQW_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP9572350.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(465..1595)
                     /gene="dnaJ"
                     /locus_tag="ACHOQW_001250"
     CDS             complement(465..1595)
                     /gene="dnaJ"
                     /locus_tag="ACHOQW_001250"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9572351.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1684..3600)
                     /gene="dnaK"
                     /locus_tag="ACHOQW_001251"
     CDS             complement(1684..3600)
                     /gene="dnaK"
                     /locus_tag="ACHOQW_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9572352.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3977..4381
                     /gene="yaaI"
                     /locus_tag="ACHOQW_001252"
     CDS             3977..4381
                     /gene="yaaI"
                     /locus_tag="ACHOQW_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP9572353.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4407..5120
                     /gene="msyB"
                     /locus_tag="ACHOQW_001253"
     CDS             4407..5120
                     /gene="msyB"
                     /locus_tag="ACHOQW_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9572354.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFVGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRQMVSATQV"
     gene            5269..5835
                     /gene="satP"
                     /locus_tag="ACHOQW_001254"
     CDS             5269..5835
                     /gene="satP"
                     /locus_tag="ACHOQW_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP9572355.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5870..6457)
                     /gene="mog"
                     /locus_tag="ACHOQW_001255"
     CDS             complement(5870..6457)
                     /gene="mog"
                     /locus_tag="ACHOQW_001255"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP9572356.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6572..7525)
                     /gene="tal"
                     /locus_tag="ACHOQW_001256"
     CDS             complement(6572..7525)
                     /gene="tal"
                     /locus_tag="ACHOQW_001256"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9572357.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7804..9234
                     /gene="yaaJ"
                     /locus_tag="ACHOQW_001257"
     CDS             7804..9234
                     /gene="yaaJ"
                     /locus_tag="ACHOQW_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP9572358.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9304..10080
                     /gene="yaaA"
                     /locus_tag="ACHOQW_001258"
     CDS             9304..10080
                     /gene="yaaA"
                     /locus_tag="ACHOQW_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP9572359.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10233..10529)
                     /gene="yaaX"
                     /locus_tag="ACHOQW_001259"
     CDS             complement(10233..10529)
                     /gene="yaaX"
                     /locus_tag="ACHOQW_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP9572360.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10743..12029)
                     /gene="thrC"
                     /locus_tag="ACHOQW_001260"
     CDS             complement(10743..12029)
                     /gene="thrC"
                     /locus_tag="ACHOQW_001260"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP9572361.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12030..12962)
                     /gene="thrB"
                     /locus_tag="ACHOQW_001261"
     CDS             complement(12030..12962)
                     /gene="thrB"
                     /locus_tag="ACHOQW_001261"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP9572362.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12964..15426)
                     /gene="thrA"
                     /locus_tag="ACHOQW_001262"
     CDS             complement(12964..15426)
                     /gene="thrA"
                     /locus_tag="ACHOQW_001262"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP9572363.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15452..15565)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15507..15590)
                     /locus_tag="ACHOQW_001263"
     CDS             complement(15507..15590)
                     /locus_tag="ACHOQW_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP9572364.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(15804..16490)
                     /gene="yjtD"
                     /locus_tag="ACHOQW_001264"
     CDS             complement(15804..16490)
                     /gene="yjtD"
                     /locus_tag="ACHOQW_001264"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9572365.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16890..17030)
                     /gene="yjjY"
                     /locus_tag="ACHOQW_001265"
     CDS             complement(16890..17030)
                     /gene="yjjY"
                     /locus_tag="ACHOQW_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP9572366.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17126..17842
                     /gene="arcA"
                     /locus_tag="ACHOQW_001266"
     CDS             17126..17842
                     /gene="arcA"
                     /locus_tag="ACHOQW_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP9572367.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17902..19254)
                     /gene="creD"
                     /locus_tag="ACHOQW_001267"
     CDS             complement(17902..19254)
                     /gene="creD"
                     /locus_tag="ACHOQW_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP9572368.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19312..20736)
                     /gene="creC"
                     /locus_tag="ACHOQW_001268"
     CDS             complement(19312..20736)
                     /gene="creC"
                     /locus_tag="ACHOQW_001268"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP9572369.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20736..21425)
                     /gene="creB"
                     /locus_tag="ACHOQW_001269"
     CDS             complement(20736..21425)
                     /gene="creB"
                     /locus_tag="ACHOQW_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP9572370.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(21438..21911)
                     /gene="creA"
                     /locus_tag="ACHOQW_001270"
     CDS             complement(21438..21911)
                     /gene="creA"
                     /locus_tag="ACHOQW_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP9572371.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22122..22991
                     /gene="robA"
                     /locus_tag="ACHOQW_001271"
     CDS             22122..22991
                     /gene="robA"
                     /locus_tag="ACHOQW_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP9572372.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22988..23635)
                     /gene="gpmB"
                     /locus_tag="ACHOQW_001272"
     CDS             complement(22988..23635)
                     /gene="gpmB"
                     /locus_tag="ACHOQW_001272"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP9572373.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23687..24199
                     /gene="yjjX"
                     /locus_tag="ACHOQW_001273"
     CDS             23687..24199
                     /gene="yjjX"
                     /locus_tag="ACHOQW_001273"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP9572374.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(24346..24672)
                     /gene="trpR"
                     /locus_tag="ACHOQW_001274"
     CDS             complement(24346..24672)
                     /gene="trpR"
                     /locus_tag="ACHOQW_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP9572375.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24762..26699)
                     /gene="sltY"
                     /locus_tag="ACHOQW_001275"
     CDS             complement(24762..26699)
                     /gene="sltY"
                     /locus_tag="ACHOQW_001275"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP9572376.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26910..28577
                     /gene="ettA"
                     /locus_tag="ACHOQW_001276"
     CDS             26910..28577
                     /gene="ettA"
                     /locus_tag="ACHOQW_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP9572377.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28684..28851)
                     /gene="nadS"
                     /locus_tag="ACHOQW_001277"
     CDS             complement(28684..28851)
                     /gene="nadS"
                     /locus_tag="ACHOQW_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9572378.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(28885..30117)
                     /gene="nadR"
                     /locus_tag="ACHOQW_001278"
     CDS             complement(28885..30117)
                     /gene="nadR"
                     /locus_tag="ACHOQW_001278"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP9572379.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30138..31520)
                     /gene="radA"
                     /locus_tag="ACHOQW_001279"
     CDS             complement(30138..31520)
                     /gene="radA"
                     /locus_tag="ACHOQW_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9572380.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31569..32537)
                     /gene="serB"
                     /locus_tag="ACHOQW_001280"
     CDS             complement(31569..32537)
                     /gene="serB"
                     /locus_tag="ACHOQW_001280"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP9572381.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32643..33284
                     /gene="ytjB"
                     /locus_tag="ACHOQW_001281"
                     /pseudo
     CDS             32643..33284
                     /gene="ytjB"
                     /locus_tag="ACHOQW_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            33315..34331
                     /gene="lplA"
                     /locus_tag="ACHOQW_001282"
     CDS             33315..34331
                     /gene="lplA"
                     /locus_tag="ACHOQW_001282"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP9572382.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34363..34626
                     /locus_tag="ACHOQW_001283"
     CDS             34363..34626
                     /locus_tag="ACHOQW_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9572383.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT         8163 a         9423 c         8650 g         8403 t
ORIGIN      
        1 tcttccggat tagaatcgat aaaacagaat gggttaaaga gagcgatata atagcggcgg
       61 gtgcttgagg ctgtctgtct caggcattag ctgaacggca gatagagaaa agccccgagt
      121 gatattttac catcaacccg aggcccccta tatgctgaac acatgtagag tgcctcttac
      181 tgaccgtaag gtcaaggaga agagagcaat gaagcagcat aaggtgatga ttgtcgccct
      241 gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta acgagaaaag acctctgtga
      301 ggttcacatc cgaactggcc agacggaggt tgctgttttc acggcttacg aatccgagta
      361 agagcaacgg cggggagtga tccccgccac tttacaggtg ctcgcatatc ttcaacgcac
      421 cctattttta cccaggcctg cccacgggca ggcttttggg gaggttagcg ggtcaggtcg
      481 tcaaaaaact tcttcacacc atcaaagaag ctctttgagc gcgggctgtt gtgctcgccg
      541 gttgggccac cgaagctttc ttgcagctct tgcagcagct gtttctgctt ctcgttcagg
      601 cctaccggtg tttcgacgac aacgcggcac agcaaatcac cctgtgcgcc accgcggaca
      661 gacttgacgc ctttaccgcg catacggaat agcttaccgg tctgggtttc gccaggcact
      721 ttcagtttga cgcgaccatc aagggtcggt acttcgattt caccacccag cgccgccata
      781 gcgaagttga tcgggacttc gcaatacagg ttgttgcctt cacgctcgaa aatcgggtgc
      841 tgtttaacct gaacctgaac gtacagatcg cctgccggtg cgccgtgttc acccgcttca
      901 ccttcgcccg caagacggat gcggtctcca gtgtccaccc ctgccgggat tttaacggac
      961 agcgttttgc tgcgctcaac acgaccatga ccatgacatt tgttgcacgg atctttgatc
     1021 agcgtaccgc ggccctgaca gtgtggacag gtctgctgca cggcaaagaa accctggcgc
     1081 atctgcacct gaccagaacc atgacaggtc ggacaggtct gcggctgtgt acctggtttt
     1141 gcaccgctac cgtggcaaac gtcacactct tccagagtcg gaatgcggat ctctttggtc
     1201 acgccacgta cagcttcttc gagggtgagc tccatgttat agcgtaaatc agcaccgcgc
     1261 gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc cgaaaacgtc accaaaaata
     1321 tcgctgaagt ctgcgccgcc gccaaaaccg ccgccgccca tgccaccttg ctcaaacgca
     1381 gcatgaccat actgatcgta tgccgcacgt ttttgcgagt cggtcagaac ttcataagct
     1441 tccttgatct ctttaaattt cgcctcggcc tctttgtcac cctggttacg gtccgggtgg
     1501 tatttcatgg ccaggcgttt gtaggccttt ttgatttcac gctcttccgc tgttttggaa
     1561 acgcctaaaa tctcgtaata atcttgctta gccatctttt ttaaattgcc cctagatgaa
     1621 tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt ataattaccc gtttataggg
     1681 cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg acaacatcgt catctttcgc
     1741 gttgtttgca gaagcatcag caccggcagt ctgctgctgg gcatgttgct gctgggcgat
     1801 ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt ttcgcttcga tagcggcttt
     1861 gtcttcacct ttcagagcag tttccagtgc agtcagcgca gactcgatag cagttttgtc
     1921 gtcagccggc agtttgtcgc ctgcttcttc aacctgctta cgggtgctgt gcagcagatg
     1981 gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta cggtcagctt cggcgttagc
     2041 ttcggcgtcg cgtaccattt tctggatttc atcttcgttc agaccagaag acgccttgat
     2101 ggtgatcttc tgctctttac cgctgttttt atctttcgcg gaaacgtgca ggataccgtc
     2161 agcatcgata tcgaaggtaa cttcgatctg cggcatgccg cgcggtgccg ggttgatacc
     2221 atccaggttg aactgaccca gagatttgtt gtcagccgca cgtttacgtt caccctgcag
     2281 cacatggatg gttaccgcag actggttgtc ttcagcggta gagaacacct ggctgtgctt
     2341 ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc acaccgccca tggtttcgat
     2401 acccagagac agcggggtaa catccagcag cagtacgtct tttacgtcac cagtcagaac
     2461 accaccctga acagcagcac cgattgctac agcttcgtcc gggttaacgt ctttacgcgg
     2521 ctctttacca aagaactcag caactttctt ctgaaccatt ggcatacgag tctgaccacc
     2581 aacgaggata acgtcgtcga tatcagatac ggacaggcca gcgtcctgca gcgcaacttt
     2641 cagcggctcg atggaacggt taaccagatc ttcaaccagg ctttccagtt tcgcacgagt
     2701 cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca gtgatgtacg gcaggttaac
     2761 gtcggtctgc tgagcggaag acagttcgat tttcgctttt tctgccgctt ctttcaggcg
     2821 ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga tctttcttga attcttcaac
     2881 cagatagttg atcagacggc tgtcgaagtc ttcaccaccc aggtgggtat caccgttggt
     2941 tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg atttcgataa tagaaatatc
     3001 gaaagtacca ccacccaagt cataaaccgc gatagtacgg ttgccagtac ctttgtccag
     3061 accgtaagcc agcgcagctg cggtcggttc gttgatgata cgttttactt ccagaccagc
     3121 gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg ttaaagtatg ccggtacggt
     3181 gataacagct tcagttaccg gttcacccag gtaatcttca gcggttttct tcattttttt
     3241 cagcacttca gcagaaatct gcggcggtgc cattttctgg cctttaactt cgacccatgc
     3301 gtcgccgtta tcagcagcaa taattttgaa cggcatgatg gaaacatcac gctgtacttc
     3361 ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca aacagggtgt tttgcgggtt
     3421 cgtcactgcc tgacgtttag ccggctgacc aaccagagtt tcaccatcct gggtataggc
     3481 aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc agcacacgag gagtggtgcc
     3541 atccataatc gctacacaag agttggtagt acccaggtcg ataccaatta ttttacccat
     3601 ctaaacgtct ccactatata ttcggtcatc atgtggttgt gagtctgtaa taggggcgaa
     3661 acgtctggtt tcaactgccc aatttcattt ttttgcagac tcactcactg cggttgacta
     3721 ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc aaaaattttt tgtgcttttg
     3781 cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt gacagcaatt ttattcatat
     3841 aaagaatgaa tcgcagtcaa ggtattcatc aaaaaattgt aataaaaaga aaagattacg
     3901 tgcctgaatc ttctctttat cagcagtaaa ctagtgggta ttcatccccc tacctcttcc
     3961 cactaagaga atccttatga aatccgtttt tacgatttcc gccagcctgg cgattagcct
     4021 gatgctgtgc tgcacggcgc aggcaaacga ccataaactc ctcggcgtca ttgcaatgcc
     4081 gcgtaacgaa accaacgctc tggcgctgaa acttcctgtt tgtcgcattg tgaaacgcat
     4141 acaactctct gccgaccatg gcgatttaca gttaagcggt gcatcggttt atttcaaagc
     4201 cgcccgtagc gccagtcaga gcctgaatat tccttcagaa ataaaagaag ggcaaaccac
     4261 tgactggatc aacattaaca gcgataacga caataaacgc tgcgtctcaa aaatcacctt
     4321 ttccggtcat acggtgaact catcggatat ggccacgctg aaaattatcg gcgacgacta
     4381 acgccagaat atagaagcca caaaaaatga atgttaatta cctgaatgat tcagatctgg
     4441 attttctcca gcattgtagt gaggaacagt tagcaaattt cgcccggctg ctcacccata
     4501 atgaaaaagg caaaactcgc ctctccagcg tactgatgcg caacgaactg tttaaatcga
     4561 tggaaggaca tcccgagcaa catcgccgca actggcagct gattgctgga gaattacagc
     4621 attttgttgg cgatagtatc gccaacaaac tgcgcggaca cggtaaattg tatcgggcca
     4681 ttttgctcga tgtttcaaaa cgattgaagc tgaaagccga caaagagatg tctacgtttg
     4741 aaattgagca acagttactg gaacaatttc tgcgtaatac ctggaagaaa atggacgagg
     4801 aacataagca ggagtttctg cacgcggtcg atgccagggt gaatgagctg gaagagctgc
     4861 tgccgctgct gatgaaagac aaattattgg caaaaggcgt gtcgcatctg ctttccagcc
     4921 aactgacccg cattttacgc acccacgcag caatgagcgt acttgggcat ggtttgctgc
     4981 gcggcgcggg gctgggaggc cctgtaggcg cggcactaaa tggggttaaa gcggtcagcg
     5041 gcagctccta tcgcgtgacg attccagccg tactgcaaat cgcctgcctg cgccagatgg
     5101 ttagcgccac tcaggtctga taccggaaac cggggaaaat tttcattttc ccctcaaaag
     5161 atcgtagaca ctgccccact ggctgattat tatgccgcgc cctgaaaaca ctacagttat
     5221 tcagggaaat tatttcacca ttcattcgat gatgattttt gaggaattat gggcaacact
     5281 aagttggcta atccggcacc gctgggcctg atgggcttcg gcatgaccac cattctgctt
     5341 aacctgcaca acgtaggtta tttcgctctg gacggtatta ttcttgccat gggcattttc
     5401 tacggcggca tcgcgcaaat ttttgctggt ctgctggagt acaaaaaagg caacactttc
     5461 ggtttaaccg cattcacctc ttacggttct ttctggctga cgctggttgc gattctgctg
     5521 atgccgaaac tgggtctgac cgatgcgcca aatgcacagt tccttggtgt ctacctgggt
     5581 ctgtggggcg tatttacgct gtttatgttc ttcggcacgc tgaaaggcgc acgcgttctg
     5641 caattcgttt tctttagcct gaccgtgctg tttgccctgc tggcgatcgg taacattgcc
     5701 ggtaacgccg caatcatcca ctttgccggc tggattggtc tgatctgcgg tgccagcgca
     5761 atctatctgg cgatgggtga agtactgaac gagcagtttg gtcgcaccgt tctgccgatt
     5821 ggtgaatccc actaattgtc ttgagatccc cccgctcggg gggatttttt tattcgctaa
     5881 cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac ttctggtgcc gtttcaacgt
     5941 atggcccttc cagcaactga atgcagtacg gtacgctggc aaaaataccg tgtaccacaa
     6001 cgttaccctc agcgtccttc acaccttcca gcgtctcttt aatagacttc ggctgaccgg
     6061 gtaagttaag gatcagcgcc tgtttgcgaa tcacacccac ctgacgcgaa aggatcgcag
     6121 ttggtacaaa atgcaggctg atctggcgca tctgttcacc aaagccaggc atctcgcggt
     6181 ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg cgccgggcca gttccgcccg
     6241 tggtgagcac cagatggcaa ctcatttcat ccaccagctc acacaacgtt tgctcgatga
     6301 tcgcctgctc atcggggatt aagcgggttt ccagttcaaa cggcgtggtt agcgccgatg
     6361 tcagccattc ttccagcgca gggatacctt tatcctgata aacgccgctg gatgcgcgat
     6421 cagagatgga aactaagcca atacgtaaag tattcatcaa gatttttccg tttaaacagt
     6481 ctcgttaaac agaatgatac actgcgaagg gagtgacaga caggcttcag aagaggtagc
     6541 gtgaccgact tcccggtcac gctaagaatg attacagcag atcgccgatc attttttcca
     6601 gtttttcctg gtcaatagca aacttacgga taccttccgc cagtttatct actgccattg
     6661 gatcctggtt gtgctgccac aggaactcgg actcagtgat acgcgccgga cgcgctttca
     6721 cttcgccggt gtaagacagt ttacgttcga tagccccttc gctctcagcc agctctttca
     6781 gcagtgccgg tgcgatggtc agacggtcgc agcctgccag ttccagaatt tcgccgatgt
     6841 tacggaagct tgcgcccata accacggttt cataaccgtg ctctttgtag tactggtaga
     6901 tttcagatac agaaaccacg cccggatctt ctgccggagc gtactctttc ttatcggtat
     6961 tcgctttgta ccagtcaaga atacggccaa caaacggcga gatcaggaac acgcccgctt
     7021 ccgcacaagc acgagcctga gcgaaggaga acagcagggt caggttacag ttgatacctt
     7081 ctttttccag ctgttccgca gcacggatac cctgccaggt agaagccagt ttgatcagaa
     7141 tacgatcgtt gctaatacct gcatcgttgt agagtttgat caggcgtttt gctttcgcaa
     7201 ttgacgcttc tgtgtcatag gaaagacgcg catcaacttc agttgagata cggcccggaa
     7261 ccagtttcag gatttccaga ccaatattta ctgccagttt gtcagtcgcg tccacgatct
     7321 gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac agcatcatca atcaacttac
     7381 ggtattccgg aatctgcgct gcgttaagaa tgagagaagg gttggttgtg gcatcctgcg
     7441 gttgatacag cttcattgcc gcgatgtccc cagtgtcggc cactacggtg gtgtactgac
     7501 gaagggaggt caatttgtcc gtcatgatag tatttctctt taaacagctt gttaggggga
     7561 tgtaaccggt ctgccctgat gatatcacga cgcaacgtca gcgcaaccgc cgacaagacg
     7621 gttatcgcca ggcgagactg tttcggattt ctgacactgc gttgatatcg cccgccattt
     7681 ttaaacaaaa cctcatgtat gctacgcaga agttatcaag tacctcgtag cgtatatact
     7741 tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc gaccggcaat aagagggata
     7801 tgcatgccag attttttctc ctttattaac agcgtccttt ggggatcggt aatgatttac
     7861 ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg gatttgtgca gtttcgctac
     7921 atccgccagt ttggcaaaag tcttaaaaat agcattcatc cacagccagg cggtttaacc
     7981 tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg gtagcggcaa cctggccggc
     8041 gttgcgctgg ctattaccgc cggtggacct ggagccgtct tttggatgtg ggttgccgcg
     8101 tttatcggca tggcgacctc gtttgccgaa tgttcccttg cacaacttta taaagaacgt
     8161 gacgtcaatg ggcagtttcg tggcggaccg gcatggtata tggcgcgcgg gctggggatg
     8221 cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg cctacggcat aattttcagc
     8281 ggagttcagg caaacgctgt tgcccgagct ctgagttttt cttttgattt tcccccgctg
     8341 gtgacaggca ttattcttgc tgtctttgct ctgctggcaa tcattcgcgg tcttcatggc
     8401 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt actgaccagc
     8461 ctggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     8521 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     8581 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg gtcgacgcca
     8641 aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg aattgtccag
     8701 atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta
     8761 ctggcgggta acggcacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
     8821 atgcgggtgc taatgggttc ctggggtgct gaatttgtta ccctcgtggt tattctgttt
     8881 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
     8941 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
     9001 ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
     9061 gctattacca atttgactgc catattactg ctctcgcctg tggttcatac cattgccagt
     9121 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
     9181 gatatcggtc gccagctttc tcgggacgcg tgggatgatg tttcgcagga gtaatcacaa
     9241 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
     9301 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
     9361 acgcgatata cactgccgga gctgttagac aattcccagc agttgatcca tgaagcgcgg
     9421 aaactgacgc ctccgcagat tagcacgctg atgcgtatca gcgacaaact ggcaggtatc
     9481 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggcg
     9541 attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt cagcgaagac
     9601 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactccgc
     9661 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
     9721 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
     9781 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
     9841 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
     9901 aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
     9961 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    10021 tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    10081 gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    10141 ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    10201 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    10261 ccgccgtgat gatcatgatg agctttctta tggtgacgcg gcggtggcgg cggtccgtgt
    10321 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    10381 tgaccgccat cccagtaata accgcgatta tcacgatcgc ctatctgtaa ttttactgac
    10441 gggactaacg taatttccgc agcctgcgtt gccatgggag cgaccagaac cagggaaagt
    10501 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    10561 gtcgatatcc ggagattacg cgatgagagc ggcggttgtt attctccgca atcctaatct
    10621 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttctc tccatatttt
    10681 cttcacaaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    10741 tgttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgc
    10801 gaaagcaagg gtaaatcggc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    10861 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    10921 tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc gtgcggctcc
    10981 gaagtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    11041 gcggcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    11101 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    11161 ggtgaccact gaccgtcatg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    11221 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
    11281 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca
    11341 actgcttcaa agtagtagca aatctgcgcc agcaaccggc taatgttaat cgagttagct
    11401 gagtttaacc ccagcgccac tttcagctct tcatcatcaa acgcctgctt caccagcgcc
    11461 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caatgtacag
    11521 aacagttttt cttgcagtgg actgattttg cctcgtggat aaaggataac cactttcaca
    11581 ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
    11641 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
    11701 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    11761 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    11821 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    11881 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc gtgcggaaaa
    11941 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctca
    12001 ttgtgatctt taagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    12061 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    12121 tcggcaacgc gctgggcggt atccggcttg tcacacagag caaacaaggt cgggccggag
    12181 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    12241 ccaggcagta accgttcacg gtagggttct gcgataacat ctttcatcag cttcgcggca
    12301 agctcaggct gacgggaata gcaggcgtga atgaagcctg ccaagtgtcg cccgtgcgca
    12361 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    12421 ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    12481 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    12541 tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc cagcaaacgg
    12601 gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca tcagagccgc gaccaccgaa
    12661 caggcgctgg agcctaagcc cgaaccgatc ggcatattct tttccagagt catcgccact
    12721 ggaatttgct tgcccagctc ctggcaaaaa cgctcccagc actgataaac gatattttcc
    12781 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    12841 gccgactcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc
    12901 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    12961 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccggcagc
    13021 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    13081 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattaccatc
    13141 cacttcggca atcttcacgc ggcagacgcc atcttcatca atattgccaa catagcgcaa
    13201 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    13261 cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg gcagcacagg
    13321 ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga gaattaatag
    13381 tttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg tataacccat
    13441 ttcccgcgcc agcgtagtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    13501 ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac cagcattgag
    13561 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    13621 tttacgccgc gatttttcag ccgcatgacg caacagatgg tagtaatcca tcgacgaggt
    13681 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    13741 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    13801 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg ccagttcttc
    13861 ctgccagttt tccagattta ggccatgcac attggtgagc agagccttcg agttagcaac
    13921 accgcagaca cgtaagtcga tatgtttatt cttcaaccag ctttgctgac gcttcagttg
    13981 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    14041 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    14101 cacgacagag attgagcgtt cagaagatcc ctgagcaata gcgacaatgt tgatattggc
    14161 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    14221 taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta agccttcttt
    14281 cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt cgctttgcgg
    14341 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    14401 ggcgcgtgac attgcagcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
    14461 gccggaaacg ctgaacattg ccatattatt cagattggaa atgcccttga ccggtaattc
    14521 gtcttcatca cgactggcac caatgagcgt acctggagct tgaggatttc cggtattttt
    14581 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    14641 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgatttca acaacctcgc
    14701 atcgggcacc tgacgcgggt cgcaggtata taccccgtca acgtccgtcc aaatctcgca
    14761 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
    14821 aagtaccacc agttcgcctt tttcattacc ggcggtgaaa cctgccatca gcaccatgtg
    14881 atcagccgga atacgacttg ccgcaatacg gcgggtggac tctgcaatat cgacagtaga
    14941 ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg taacgttgtg
    15001 accgcgcgct tctaatacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    15061 cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg
    15121 ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg
    15181 ctgggcggcg gcgagtcccg tcagaagttc ggcaaaaata cgttcggcat cgctgatatt
    15241 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    15301 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    15361 atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    15421 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg
    15481 gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt aatggtaatg
    15541 gtgatggtgg tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
    15601 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
    15661 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctgtcag
    15721 agactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
    15781 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
    15841 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg taaccagtcg
    15901 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatggc tcgttcgcgt
    15961 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgtcggttg ttgtattaat
    16021 attgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
    16081 gccaccatcg gcacaccagt aagaacgtca gccaacgcta actcttcgtt agtcagcccg
    16141 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
    16201 agcggcacca gttctactgg cgtagcgtag taatgatatt tcgcccgact gcgcgcagtg
    16261 gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa aactttaata
    16321 ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc caggtgtgcc
    16381 tgactatcga caatccgcag ttcgctaaac cccatcgttt tcattgcccg cgccgctgcc
    16441 ccaatatttt ctgctctggc gggtgcgacc agaataatcg ttatacgcat attgccactc
    16501 ttcttgacca aatgaccgcg aacagggtga tcactgtcaa cttattacgc ggtgcgaatt
    16561 tacaaattct taacgtaagt cgcagaaaaa gccctttact tagcttaaaa aaggctaaac
    16621 tatttcctga ctgtactaac ggtttaattg ttaaaaaatg ctacatatcc ttctgtttac
    16681 ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt
    16741 atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt agatacgatg
    16801 atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt
    16861 ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca
    16921 gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg
    16981 ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga
    17041 tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat
    17101 ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga
    17161 gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga
    17221 agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat
    17281 catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca
    17341 ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg
    17401 cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat
    17461 tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg
    17521 tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc gttctttgat
    17581 cggccctgat ggcgagcagt acaagctgcc gcgcagtgag ttccgcgcca tgcttcactt
    17641 ctgtgaaaac ccaggcaaaa ttcagtcccg tgctgaactg ctgaagaaaa tgaccggccg
    17701 tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt
    17761 cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag gttatcgctt
    17821 ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaaacgg cgctttttag
    17881 cgccgttttt atttttcaac cttatttcca gatacgtaat tcatcgtccg ttgtaacttc
    17941 tttactggct ttcattttcg gcagtgaaaa cgcataccag tcgatattac gagtcacaaa
    18001 catcatgccg gccagcgcca ccaccagcac actggttccc aacaacagcg cgctatcggc
    18061 agagttgagg agtccccaca tcacaccatc cagcaacaac agcgcgaggg taaacaacat
    18121 gctgttgcgc caacctttca ataccgcctg caaataaata ccgttcatta acgccccaat
    18181 cagactggcg attatccatg ccacggtaaa accggtatgt tcagaaagcg ccagcaagag
    18241 caaataaaac atcaccaatg aaagccccac cagcaaatat tgcattgggt gtaaacgttg
    18301 cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc agcgcaatca gcagaatggc
    18361 atacttagtc gcccggtcag ttaattggta ttgatcggcg ggcgtcgtca ctgcgacgct
    18421 aaacgcgggg aagttttccc agccggtatc attgcctgaa gcaaaacgct caccgagatt
    18481 attagcaaac cagctgcttt gccattgcgc ctgaaaacct gactcactaa cttcccgttt
    18541 ggctggtaga aaatcaccta aaaaactggg atgcggccag ttgctggtta aggtcatttc
    18601 gctattacgc cccgcaggca ccacagaaag atcgccggta ccgcttaaat tcagtgccat
    18661 attcagcttc aggttctgct tccgccagtc cccttcgggt aaagggatat gcacgccctg
    18721 cccgccttgc tctaacccga tgccgggttc aatagtcagc gccgttccgt taacttcagg
    18781 cgctttcacc acaccaatac cacgcgcatc cccgacgcta atcacaataa atggcttgcc
    18841 aaaggtgata tttggcgcgt cgagttcgct aagacgcgaa acatcgaaat cggcttttag
    18901 cgttaaatca ctgtgccaga cctgaccggt ataaatccct atcttgcgtt cttccacgtt
    18961 ctgattgcca tcaaccatca atgactcagg taaccaaaaa tggataaaac ttcgtttccg
    19021 ctccacggtt ttatcctctt cctgcaccgt ataaagctcg gtcacaggaa tagcgataag
    19081 aggcccaacg agtttttgcg gcccgctggt actttggcga atcgcatctt ccacatcgct
    19141 acggtaatcg gcacgttcga caatcacctg ccgaatcagc attatcggaa tcaacaacag
    19201 caatactgca ccaaacaggg tggtcatttt ccagaacagg ggggatttca acatagtctt
    19261 ctcctttgca atggcagcag gatacgaagg ctatgtggga agaatttgaa gctatgtgaa
    19321 gtgacggtga agccgaagcg aggccagcac gccaccttcc tgcacgttgc gcagcgtgac
    19381 ttcgccgtta aacaaacggg cgacctcact gacaaacgcc aaccccagac cgctgctttt
    19441 ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc
    19501 aggaatacca ctaccggtat ccagcacctt aagggcgacg tgttcctgat ccacttcggc
    19561 gcttagcgtt atgcgaccgc tcttgggggt aaaatcgatg gcgttatcca gtaaattccc
    19621 cagcgcctgc tccagtaacg ccggttcagc agcaacgtta acctcagtag gcataacatg
    19681 caaagtgatg tttttttctg ccaactgcac ggtgcgcgct tcgctgacgc ggcgaaataa
    19741 tgccgccaca tcaacagcag tcagaacgac ttcctgacga ttctccagtc ttgcctggcg
    19801 tagtaacgtt tctaccaatg cctgcatacg cgcattttgc gtcagaatgt tgtcggtaaa
    19861 acgagccacc acttccggtg gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc
    19921 cgccagtggg ctttttagct catgggttaa cgcataaaca tactgctcaa tatagttttt
    19981 cccttccagc ttcacgcgca tactttccag cgcctgcgca agcttacgca actcgctact
    20041 gccgagttca ggaagaggaa cgggcttatt gtcagtgacg gaatcggcat agcgagtgag
    20101 ccgggcaatg gagcggttga tccaccaaac catgcctgcg ccaatcacca gtgcaatccc
    20161 cagcaaaatg gcgctggccc ataaaattcg ccgctcgctg cgcttaatga ccggagccat
    20221 cgccgcgttc ggtttgccta cgctcaaaac gccaataagc cgcgagccgt ccataatcgg
    20281 cgcggcaaca tacatcaccg aactttcggg atcggcagga ttttgcaacg tgctgcgcgc
    20341 accatactga ccacgcaacg ttagccagac gtcattccag cgcgaataat cctgcccaac
    20401 ggctttattt gccgaatcga acaatacttt gccctgcgca tcggtcatat agacatgata
    20461 ttcattgcgc actttgttaa tgccaccgat attagcgcga aacgggcgat gttgtagctg
    20521 attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc
    20581 cagctccgcc agcaacgttg cggtgtcgat cagcgtcccc tccgttgctc ttcgcacgcc
    20641 cggtttaact tctttgacaa aaatagccag tacgaaccag gccgccaccg ccaccagtaa
    20701 aaaatagccc agcaacaacc gcatgccgat acgcattaca ggctcctcag gctgtatccc
    20761 atgccgcgat gagtattaat cggtgaaaga tcggggttga tggcgcgcag cttggcacgc
    20821 agcgttttaa tgtgggtatc gacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg
    20881 atatccatca gttgctggcg ggaccagacg cggcctggcg acttgagtaa cgtcttcagc
    20941 aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg
    21001 ggttcattca attcaaaatg accaatacgg atgacgggag acggcgtcga gaacttcttc
    21061 acccgacgca gcaaggtgcg caccctagcg cacacttcgc ggggtgaaaa cggtttagcc
    21121 acatagtcgt cagcgccaat ttccagccca agcaggcgat cgacctcttc acttcgggcc
    21181 gtcaggaaca gtacaggtaa cgccggatgg agcgccagta actggcggca caattcaaag
    21241 ccactgatat caggcaaacc aacgtcgaga atcatgacat cgggaacctg ctggcgagct
    21301 ttatccagca ccggcaagcc tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac
    21361 atgtagacca gcgtgtcggc tatcccttgc tcatcttcca ctaaccagac cgtttcccgt
    21421 tgcataaatc gcctctgtta ttgtcgccac ggcatgacag gaaccgcgct aatcgcgttt
    21481 ttcggcgaac cttctacaac tttgtcggag taagccagat aagcgagcgc gttgcgtttg
    21541 gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat
    21601 actacctcgc cctgagcttt gccgttttta atacgatccg acagttcaat cggcccgact
    21661 tgctgacaag aaatagccgc atcggaggta tcttccgcca gacccaatcc ccctttaata
    21721 ccacctgttt tcgcccggct cacataacag gtgacatttt tcacatcggg atcgtcaaag
    21781 gcttccacaa caattttgtg atccgggccg atcattttaa atacggtgtc gaccgaacca
    21841 atctcttctg catgagccaa tggccccagc attatcaggc ttaaagacag gatcaaatgc
    21901 ttgtatttca tattgttacc atttttatct attacacgag caattagttc gtcacggtaa
    21961 ataacatatt gattaagaac aaaatctcaa tacttttatt tccgtcaaga cctaaaacat
    22021 actctactaa gaaaaaaaca ctgaatgcta aaacagcaaa aaatgctatt atccaattac
    22081 ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt tatggatcag gccggcatta
    22141 ttcgcgacct tttaatctgg ctggaaggtc atctggatca gcccctgtcg ctcgacaatg
    22201 tagcggcgaa agcaggttat tccaagtggc acttacagag aatgtttaaa gatgtcactg
    22261 gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac
    22321 gcctgactgc gcgtccgatt ctggacatcg cgctgcaata ccgcttcgac tctcaacaga
    22381 catttacccg cgcattcaag aagcagtttg cccagactcc tgcactttac cgccgttctc
    22441 ctgaatggag cgcctttggt attcgcccgc cgctgcgtct gggtgaattc actatgccag
    22501 agcataaatt tgtcaccctg gaagatacgc cgctgattgg tgttacccag agctactcct
    22561 gttcgctgga gcaaatctct gatttccgcc atgaaatgcg ttatcagttc tggcacgatt
    22621 ttctcggcaa cgcgccgacc attccgccag tgctctacgg cctgaatgaa acgcgtccga
    22681 gtcaggataa agacgacgaa caagaggtat tctataccac cgcgttagcc caggatcagg
    22741 cagatggcta tgtactgacg gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt
    22801 ttacctatga aggtctggga accggcgtgc aggagtttat cctgacggta tacggaacgt
    22861 gcatgccaat gctcaacctg acgcgccgta aaggtcagga tattgagcga tactacccgg
    22921 cagaagacgc caaagcggga gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta
    22981 tccgccgtta acgctgcagc tcatctaatg caggggcgtc cagatgcgag atgtcccctg
    23041 cggtttcaac gacccagcca gacgccagcc acaggctttc ctgataatcc acgcgcgaaa
    23101 tagaacagtt acgcagacgt aagcggcgct ctgcccatgc tggtaatccg agaatcgtac
    23161 tcaccagaca tcccagtgca ataccatggc tcaccagcag cggtcggctt ccctgcggta
    23221 agtcccggca agattccagt gccgcattga cgcgatcgct gagctcctgc attgactcgc
    23281 cttcaggaat acgcccatca acggtgccat tgaccagctg ccgacgccag ttctcttctt
    23341 cttcggtcag agaatcgata tgtctttttt ccagcacacc catgtttaat tcacgcaggc
    23401 gagaatcaaa gatgatgtca cagccgcagg cctgggcgat gatttccgcc gtacgccggg
    23461 tgcgtcctaa atcgctactg atgatatgcg taatgccaag ctctttggca cgcgtcgcca
    23521 cctgcatcgc ctgttgctca cctttggcgg tcagcgggct gtcagactgg ccctgaatac
    23581 gtcgctcggc gttccactgc gtttcaccgt ggcggactag gtatacctgt aacatgcttt
    23641 ttttccgtat actgctgtaa cgttaatttt ctgagtaatg ctgattatgc accaagttgt
    23701 ctgtgcgacc accaatcccg ctaaaattca ggccattctg caggcatttc acgagatctt
    23761 cggcgaagga tcctgccata ttgcatccat tgccgtcgag agcggtgtac cggaacagcc
    23821 ctttggcagt gaggaaacgc gcgctggcgc acgaaaccgg gtagccaatg cccgccgttt
    23881 acttccagag gctgattttt gggtggcgat tgaagctggc attgatggcg acagcacttt
    23941 cagttgggtg gtgattgaaa acaccaccca gcgcggcgag gcgcgttcgg caactttgcc
    24001 gttgcctgcg gtgatattgg aaaaagtacg tgaaggtgag gcgcttggtc ccgtgatgtc
    24061 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    24121 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    24181 tcataatgcg gtgtattaag cgcctgatgc gacgctgccg cgtcttatca tgcctaccaa
    24241 acatattgaa attacgggta tttgtaggac ggataaggcg ttcacgccgc atccggcatt
    24301 cggtgcacga tgcctgatgc gccacgtctt atcaggccta caaaatcaat cgcttttcag
    24361 caacacctct tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc
    24421 acgcgtaatc gtcgcgatgc ctgcgccgag ttcatttttt aactcacgct ggctcatttc
    24481 gccgcgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg
    24541 cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag
    24601 gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga
    24661 ttgttgggcc ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg
    24721 tactcgctaa agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat
    24781 tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat agcggtagta agcgtcataa
    24841 gccagcacgt tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc
    24901 acagcgtcga tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg
    24961 ttataagctg ctgaggagaa aatacggtta ttaccaaatt gctgataaac atattgcagg
    25021 taactggtgc caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa
    25081 ccaggaatag agaacatctt caccgtatgg gtcgccgtgc ccggcataat ctgcatcagg
    25141 ccgctggccc ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc
    25201 gccatcgcat agctttgcgg gatctccttc ccactggtgt aacgtttgaa aagatcgttg
    25261 taagccagcg ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga
    25321 acgctaagat cccaccattg gttgttgaaa gcataccgcg ccagttgagc ctgctctgtt
    25381 tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag
    25441 tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc
    25501 tgcggcgctt tatcaatctt cagctcatac tcttcgccga tgcgttgtgc agcaaccatc
    25561 gggtagaaac cacgctgttg catgagttga tgcaaaatct cttttgcttc agcttcacgt
    25621 ccgcgttcca gcaataaatc cgcctgccag taacgccatt catctttctc tttcgcttcc
    25681 ataggcagac gcgccagcca ggtattcagg ccgcgacgat cgccagtgcc aagcgccatt
    25741 cgcacacggc gttcaataag cgaagtagat tgcgagcgca taatggcgtc atcgcgccat
    25801 ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc
    25861 tcctgaatct gatcttcatt aagctgctgc gcctgggcaa gcgatgggat catcagccgt
    25921 gcattttcag catcctgccg cgccacactg gcaaacgcca ccgccgccat ttgacgggta
    25981 aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg tattggggtt gttcgccagt
    26041 gaaatgattg ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt
    26101 accaggccag tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga
    26161 tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc
    26221 ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc
    26281 catttcgcat agtagtaatt acattgtgct tcggtagttc cgggcttttc cgggctaaag
    26341 gctaacaggc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc
    26401 gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt
    26461 tgattcatca ggtcatcggt gatctggcgg tattccaggt agggataaag cggataatcc
    26521 ttcagtccag gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg
    26581 atttgcgcgt aacgactacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc
    26641 gtcagcagac agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca
    26701 agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc
    26761 taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgagggt
    26821 gatcgaccgg ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca
    26881 tcacgataaa catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg
    26941 tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc
    27001 ctggggcaaa aattggtgtc ctgggtctga acggcgcggg taagtccacc ctgctgcgca
    27061 ttatggcggg cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga
    27121 ttggttatct gccgcaggaa ccgcagctga acccggaaca caccgtacgt gagtccattg
    27181 aagaagcggt ttcagaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt
    27241 acgccgatcc ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga
    27301 tcattcaggc tcacgacggt cataatctga acgtacagct ggagcgtgcg gcggatgcgc
    27361 tacgtctgcc ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg
    27421 tggcgttgtg ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca
    27481 accacctgga tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgaaggca
    27541 ccgtggtggc gattacccac gaccgttact tcctcgataa cgttgcgggc tggatcctcg
    27601 aacttgaccg cggtgaaggt attccgtggg aaggtaacta ctcctcctgg ctggagcaga
    27661 aagatcagcg tctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga
    27721 aagagctgga gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc
    27781 tggcacgctt tgaagaactg aacagcaccg aatatcagaa acgtaacgaa accaacgaac
    27841 tgtttattcc acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta
    27901 aatcctatgg cgatcgtctg ctgattgatg acctgagctt ctcgatcccg aaaggagcga
    27961 tcgtcgggat catcggtccg aacggtgcgg gtaaatcgac cctgttccgt atgatctctg
    28021 gtcaggaaca gccggacagc ggcaccatca ctttgggtga aacggtgaaa ctggcgtcgg
    28081 ttgatcagtt ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg
    28141 ggctggatat catgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct
    28201 ttaactttaa aggggttgat cagggtaaac gcgttggtga actctccggt ggtgagcgcg
    28261 gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac
    28321 caaccaacga cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc
    28381 cgggctgtgc gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca
    28441 ttctggatta ccaggatgaa ggtaaagttg agttcttcga aggtaacttt accgagtacg
    28501 aagagtacaa gaaacgcacg ctgggcgcag acgcgctgga gccgaagcgt atcaagtaca
    28561 agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg cgaacgcctt atccggccta
    28621 caaaaccagc aatttcaata cgttgtaaaa ctgtagacct gataagacgc ggtaagcgtc
    28681 gcatcaggca ttgagcaccg aatgccggat gcggcgtgaa caccttttcc ggtctacaac
    28741 agccttagca tcctcaattt cgtggcgggc cacccgtgca ggttttttca aaccttgttt
    28801 aatctcgaca gcctcttcca gagaggtttt caactcatca aaaaaactca ttttatacct
    28861 cgcctttgag cagtttcatc acggttatct ctgctccccc atcatctccc gcaccagctc
    28921 cacgcagcgc aggaaacggc tgtcgtaatc ttcctcttca acccgcacaa attcgatatt
    28981 gttctcttcc agcatctcca ccagcagatt ctggaactct ttacgatcca ccgaactgcc
    29041 gaggctgcgt aaaccatcca ccacccacgg cgtgttgttc tccagcagga tcaccagatc
    29101 gaaacggtat tcatcaatca gcgcctgcac gaacggatgt tcccgccctt cgtacttttt
    29161 gcagaacgcc tgagtggtga caaaatcggt atcgataaat gccactttat tggcatattt
    29221 caccgcaaaa tcaatgtatt gagcgtggcc cagcgcgatt ttatcgtagt cagaatactg
    29281 caatgcgatc tcatcaccgc cgaggtgtga aaagacataa tcgcggccat attcccacgc
    29341 actggtggtg ttgaagatat tggcaagttt gtttaccagg gtggatttac cgctcgactc
    29401 gccgccaagg atcgccacgg tacgcacaaa aaacggcttc acttcggtag gaatatattc
    29461 ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg atactcataa aggtacgttt
    29521 cggatcgacc agcaccgtct cgatccccag atgttccata tactgtggcg catcggcttc
    29581 ttccgaggtg tagatcagat ccggctggat ccccttttca gccataaact ttttgatacc
    29641 gttgctccac acatcccagc cgtgcggata cggctccatg ccctcttcgt tgaaagcatg
    29701 aatgcgaata tttttctgat atttaaaagt ttgcaataac cagcgcagac gatccggcac
    29761 ggtcggctgc tgcgacatgg cactgtcttc gaacaacgcg cggtcacggg tatcgtcaaa
    29821 acccataatg atatgcagct cgtcaacctg gctacaggcg cgctggataa ggtagatatg
    29881 tccggtatgc agtgggtaga acttaccgaa tacgacaccg atcgttttct tctgccgggg
    29941 aaactcaagc cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg ggcttttgat
    30001 tttggcattc agtaactggc ttaaataccc tttggtcata ccgctggcat cagctacctg
    30061 ctgtagcgtg cagccctgtt gcttgatggc agttttcagg taatcaaatg acgacatatc
    30121 tccctccgta tttctcatta taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta
    30181 acgccaaaaa tctgcatccc ttccggcgct tttttcggta cgttagccgc cggaacaatc
    30241 gcccggcgaa aaccgtgttt cgccgcttca gagattcgtt cctgaccgct gggcaccggg
    30301 cggatctccc ctgccagccc gacttcacca aacaccacca gatcctgcgg tagcggtctg
    30361 tcacgcaggc tggaaaccat cgccagcagt aacgctaagt cggcgctggt ttcggttacc
    30421 tttacgccgc cgaccacgtt cacaaacaca tcctgatcgg ccatttgcag accaccgtga
    30481 cggtgcaaga cagccagcag gattgccaga cggttttgtt ccagccccac tgccacgcgg
    30541 cgcggattcg ccatcatcga gtgatcgacc agcgcctgaa tctccaccag caacggacgc
    30601 gttccttccc acaccaccat cacggagctg ccggaggtca cttcatctcc gcgacttaag
    30661 aaaattgccg aagggttgct gacttcacgc agcccctgtt cggtcatcgc gaagacgccc
    30721 agctcattca ccgcgccgaa gcggttttta tggctgcgca aggtgcgaaa acgggagtcg
    30781 gcatcgccat ctaaaagcac cgaacagtcg atacagtgtt ccagcacttt cgggccagcc
    30841 agcgaaccat ctttggttac gtgccccacc atgacaatcg ccacaccgcg cgttttggcg
    30901 aagcgtgtca aataagccgc cgtttcacgc acctgcgcca cgctaccagg cgatgactgt
    30961 acatccgcca tatgcatcac ctggatcgag tcaattacca tcagcttcgg ttgctcttct
    31021 tcggcaatca ggcagatctg ttcgatgctg gtttccgaca acatattgag attgtcagtc
    31081 ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca gcgactcttc gccggtgaca
    31141 tacagcgttt tcatctgctg ggccagtttg cacagcgttt gcagtagcag cgtggatttc
    31201 cccgcaccag ggttaccgcc aatcagaatg gcacttcctg gcaccacgcc gccgcctagt
    31261 acgcggtcga actctttaaa tccggtggaa aaacgcggca gctcttcaag gctgatatcg
    31321 gagagtttct ggacttttgc caccccggcg ctaccggcat agccgctgag acgctcgtta
    31381 cgcgccaccg taggcgacgc agcaagacgc acctcggtga tggtgttcca ggcatgacag
    31441 gcactgcact gcccctgcca gcgcggataa tcggccccgc attcattaca aacaaaggcg
    31501 cgttttggag cttttgccac ggtgtacctc gttaatgctg tgccgcccgc aggatggcgg
    31561 gcgagcaatt acttctgatt caggctgcct gagaggatgc agaatacccc catcaggtca
    31621 gcgtgacgga tggtgacttc cgccttttca ttcacttttg gcttggcatg gtaggcaatc
    31681 cccagccctg ccgctttgat catcggcagg tcattggctc catcgccaat cgccacggtc
    31741 tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca gagttttcgc tttgtactgc
    31801 gcgtctacga tgtcgccgat cacattgccg gtaaatttac cgtccatgat ctccagttca
    31861 ttggctacca cggctgtcag gcgcagcttg tcgcgcaggt attcagcaaa gaaagtaaag
    31921 ccgccggagg caatcgccac tttccagccc agcgtttcca gcttgagcac cagttgcgtt
    31981 aagcctggca tcagcggcag attttcacgc acctgttgca gaatattggc gtcagcgcct
    32041 ttcagcgtcg ccacgcggct gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc
    32101 cgttcggtta cttccgccac catctcgccc gttccggcca gtttggcaat ttcatcaata
    32161 cattcaatct ggatggcggt ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg
    32221 tgcgggattt ttcccagcgg cgcgacatcc agctgcgctt cgtgggccag gcgtgtagcc
    32281 cgtgcggtga gtgaacctgc cagacgaatc acctgataat cttccacgca ccaggcggca
    32341 acaatcacca tcgccgcacc cagtttgctc tggtattggg tcagacgctg tttatccagc
    32401 ccacgaccat acagcagcca gccgctacga cctgcgtggt aatccagtgg catcacttca
    32461 tcaccactta atgaaagagg cagacccggc cataaagaga catcttcagg caggtcgcac
    32521 caggtaatgt taggcattaa ggctcctgta aaatcgttcg aagcagggaa aataacgcat
    32581 gaggctacct tgtatccatt gcttctggca acattaagtc tcaaattttc aaagggtgga
    32641 agatggctcg cacaaaactg aaattccggc tgcatcgggc agtgattgtc ctgttctgtc
    32701 ttgccttgtt agtggcactg atgcaggggg cgtcatggtt tagtcaaaac caccagcgac
    32761 agcgtaatcc acagctggaa gaactggccc gcaccctggc gcgtcaggtg acgctgaacg
    32821 ttgcaccgct gatgcgtacc gactcaccgg atgaaaaacg cattcaggcg atcctcgatc
    32881 agttaacgga tgaaagccgt atcctcgacg cgggtgtgta tgacgaacaa ggcgatctta
    32941 tcgcacgttc tggcgaaagc gtcgaagtgc gcgatcggct ggcgctcgac ggtaaaaaag
    33001 caggcggcta ttttaaccag cagattgtcg agccaattgc cgggaaaaac ggaccgctcg
    33061 gctatctgcg cctgacactc gacacccata cgctcgccac cgaagcccaa caggtggata
    33121 acaccactaa cattttacgc ctgatgttgc tgctctcact ggcaatcggc gtagtgctga
    33181 cccgcacgct gctacagggt aaacgcaccc gctggcagca atcgcccttc ctgttaaccg
    33241 ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa agagtgcccc attactacaa
    33301 gaaaggaaat cgttatgtcc acattacgcc tgctcatctc tgactcttac gacccgtggt
    33361 ttaacctggc ggtggaagag tgtatttttc gccaaatgcc cgccacgcag cgcgttctgt
    33421 ttctctggcg caatgccgac acggtagtaa ttggtcgcgc gcagaacccg tggaaagagt
    33481 gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt agcggtggcg
    33541 gcgcggtgtt ccacgatctc ggcaatacct gctttacctt tatggctggc aagccggagt
    33601 acgataaaac catctccacg tcgattgtgc tcaatgcact gaatgcgctc ggcgtcagtg
    33661 ccgaagcgtc cggacgtaac gatctggtgg tgaaaaccgc cgaaggcgac cgcaaagtct
    33721 caggatcggc ctatcgcgaa accaaagatc gtggcttcca ccacggcacc ttgctgctca
    33781 atgccgacct tagccgcctg gcaaactatc tcaatccgga taaaaagaaa ctggcggcga
    33841 aaggcattac ttcagtgcgt tcccgcgtga ccaacctcac cgagctgctg ccggggatcc
    33901 cccatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat ggcgagcgcg
    33961 tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc gccgaaacct
    34021 ttgcccgcca gagtagctgg gagtggaact tcggtcaggc tccggcattc tcgcatctgc
    34081 tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa aaaggccata
    34141 tcacccgcgc ccaggtgttt accgacagcc tcaaccccgc gccgctggaa gccctcgccg
    34201 ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc gaagcgctgt
    34261 tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg atagcggggg
    34321 cggtaaggta actcgctgat taaaatatcc aaggaaggaa atatgatcga tctggaaaac
    34381 caggaacggg agattatcaa tttaatgttc agccagcgta tttcctggct agccgccgtg
    34441 cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat ctcgattaat
    34501 tctctaaaac agatcgaaaa gacggaacgg cttagctcaa acatcaaaag caaaatggca
    34561 gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac ggctgaacat
    34621 aaatgaatag attcgtgaa
//
LOCUS       DAZKFZ010000028        34600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000028 DAZKFZ010000000
VERSION     DAZKFZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(73..1326)
                     /gene="amiC"
                     /locus_tag="ACHOQW_001284"
     CDS             complement(73..1326)
                     /gene="amiC"
                     /locus_tag="ACHOQW_001284"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP9572384.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1558..2889
                     /gene="argA"
                     /locus_tag="ACHOQW_001285"
     CDS             1558..2889
                     /gene="argA"
                     /locus_tag="ACHOQW_001285"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP9572385.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2951..4777)
                     /gene="recD"
                     /locus_tag="ACHOQW_001286"
     CDS             complement(2951..4777)
                     /gene="recD"
                     /locus_tag="ACHOQW_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP9572386.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4777..8319)
                     /gene="recB"
                     /locus_tag="ACHOQW_001287"
     CDS             complement(4777..8319)
                     /gene="recB"
                     /locus_tag="ACHOQW_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP9572387.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8312..11200)
                     /gene="ptrA"
                     /locus_tag="ACHOQW_001288"
     CDS             complement(8312..11200)
                     /gene="ptrA"
                     /locus_tag="ACHOQW_001288"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP9572388.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11376..14744)
                     /gene="recC"
                     /locus_tag="ACHOQW_001289"
     CDS             complement(11376..14744)
                     /gene="recC"
                     /locus_tag="ACHOQW_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP9572389.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14757..15080)
                     /locus_tag="ACHOQW_001290"
     CDS             complement(14757..15080)
                     /locus_tag="ACHOQW_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP9572390.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(15065..15472)
                     /locus_tag="ACHOQW_001291"
     CDS             complement(15065..15472)
                     /locus_tag="ACHOQW_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP9572391.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15469..16032)
                     /locus_tag="ACHOQW_001292"
     CDS             complement(15469..16032)
                     /locus_tag="ACHOQW_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9572392.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16023..16493)
                     /locus_tag="ACHOQW_001293"
     CDS             complement(16023..16493)
                     /locus_tag="ACHOQW_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9572393.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16677..17471)
                     /gene="thyA"
                     /locus_tag="ACHOQW_001294"
     CDS             complement(16677..17471)
                     /gene="thyA"
                     /locus_tag="ACHOQW_001294"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9572394.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17478..18353)
                     /gene="lgt"
                     /locus_tag="ACHOQW_001295"
     CDS             complement(17478..18353)
                     /gene="lgt"
                     /locus_tag="ACHOQW_001295"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9572395.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18504..20750)
                     /gene="ptsP"
                     /locus_tag="ACHOQW_001296"
     CDS             complement(18504..20750)
                     /gene="ptsP"
                     /locus_tag="ACHOQW_001296"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9572396.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20763..21293)
                     /gene="rppH"
                     /locus_tag="ACHOQW_001297"
     CDS             complement(20763..21293)
                     /gene="rppH"
                     /locus_tag="ACHOQW_001297"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP9572397.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21646..21792)
                     /gene="ygdT"
                     /locus_tag="ACHOQW_001298"
     CDS             complement(21646..21792)
                     /gene="ygdT"
                     /locus_tag="ACHOQW_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP9572398.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21978..22667
                     /gene="mutH"
                     /locus_tag="ACHOQW_001299"
     CDS             21978..22667
                     /gene="mutH"
                     /locus_tag="ACHOQW_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP9572399.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22736..23449
                     /locus_tag="ACHOQW_001300"
     CDS             22736..23449
                     /locus_tag="ACHOQW_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9572400.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23587..23805
                     /gene="ygdR"
                     /locus_tag="ACHOQW_001301"
     CDS             23587..23805
                     /gene="ygdR"
                     /locus_tag="ACHOQW_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP9572401.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23913..24953
                     /locus_tag="ACHOQW_001302"
     CDS             23913..24953
                     /locus_tag="ACHOQW_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP9572402.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="ACHOQW_001303"
     CDS             complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="ACHOQW_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP9572403.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26171..28330)
                     /gene="aas"
                     /locus_tag="ACHOQW_001304"
     CDS             complement(26171..28330)
                     /gene="aas"
                     /locus_tag="ACHOQW_001304"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP9572404.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            28916..29947
                     /gene="galR"
                     /locus_tag="ACHOQW_001305"
     CDS             28916..29947
                     /gene="galR"
                     /locus_tag="ACHOQW_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP9572405.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29954..31216)
                     /gene="lysA"
                     /locus_tag="ACHOQW_001306"
     CDS             complement(29954..31216)
                     /gene="lysA"
                     /locus_tag="ACHOQW_001306"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9572406.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31338..32273
                     /gene="lysR"
                     /locus_tag="ACHOQW_001307"
     CDS             31338..32273
                     /gene="lysR"
                     /locus_tag="ACHOQW_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP9572407.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSHTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32260..32952)
                     /gene="ygeA"
                     /locus_tag="ACHOQW_001308"
     CDS             complement(32260..32952)
                     /gene="ygeA"
                     /locus_tag="ACHOQW_001308"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP9572408.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33081..34499)
                     /gene="araE"
                     /locus_tag="ACHOQW_001309"
     CDS             complement(33081..34499)
                     /gene="araE"
                     /locus_tag="ACHOQW_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP9572409.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8474 a         9516 c         8548 g         8062 t
ORIGIN      
        1 ttattgaaca ccctaacggg tgtttttttg tttctggtct cctataaaaa agcgccattc
       61 agcgcctttt tatcatcccc ttctcgccag ggtcgcccca tcggcaaaat acgctttaat
      121 tcccgcaaga atagactccg caacttcctg ctggaaggtc gccgttttca gtttacgctc
      181 ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt
      241 tagtacggca aacccggcct gttcaacttg atttttatgc agcttgttga ttttaccgag
      301 cttattcagc accgctttac caaacttaag gctgtcagca atggtcagcg actgcaccat
      361 atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc
      421 ggaggcgttc tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga
      481 gagcgcaaac accgaggaac cgctcggctg gcgactggta aaggcgtcgg catggataga
      541 gacaaacagg tcagcacgct gtttctgagc ttttgctacg cgcacttgca acggaatgaa
      601 gatgtcttca ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg
      661 caaacggcga gctatttgca ataccacatc tttttcgcgc gttttgtatt tccccaccgc
      721 accggagtct tcgccaccgt ggccagggtc aagcataatg acaatcggac gatcgcgccc
      781 tgctttacct ggttgtggac cactttgcgc tggcggcacc tgcttttcga ggtcgccttt
      841 gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata
      901 gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg
      961 ttttacgttt tgctttaatt caaaaaccat ccgtacggtt tgcgggtcaa attgcccgac
     1021 gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac
     1081 cgagttcagg tttacatctt cgatatccac caccacgcgt tccgggttac tcaacgcgaa
     1141 ctgcttatat ttcagctgac gatttgattc taccgtcacg cgggtgtagc tggacgcagg
     1201 ccagacgcgc accgccacga cctggctgac cgcagccaga ctgacctgac ttacgctcaa
     1261 tagccacata gcacccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc
     1321 tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg
     1381 accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt
     1441 tatattcacg ccattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct
     1501 gcgaaaaaac agaataaaaa tacaataaat tcgaataata atgcaaagag gtgtgccgtg
     1561 gtaaaggaac gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat
     1621 acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat
     1681 ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg
     1741 gtctatggcg cacgtccgca gatcgacgca aatctggctg cgcatcacca cgaaccgctg
     1801 tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg
     1861 ggaacattgc aactggatat tactgctcgc ctgtcgatga gtctcaataa cacgccgcta
     1921 cagggcgcgc atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc
     1981 gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc
     2041 catcgtcaac tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact
     2101 ggcgagagct ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa
     2161 gctgagaaga tgattggttt ttgctcttcc cagggcgtca ctaatgacga cggtgatatt
     2221 gtctccgaac ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc
     2281 gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc
     2341 gtgcgtcgct gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc
     2401 tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca
     2461 acaatcaacg atatcggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt
     2521 ctggtacgcc gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag
     2581 cgcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg
     2641 gaaatggcct gtgtggcagt tcacccggat taccgcagct catcacgggg cgaggttctg
     2701 ctggaacgca ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc
     2761 acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttattg
     2821 cccgaaagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat
     2881 ttagggtaag cgatgaaaat cgtcggatgc gacatgcgta acactcgtac gtcgcatccg
     2941 gcaattacgt ttattcccgt gaactaaaca acgccgccag accactgcgc cgctcagtac
     3001 gagtggcgat tgccgcactt aatatgcgct catcggcata cagcgacaga cggcgacgcg
     3061 cgcgggtcac cgcggtataa accagctctc gcgttactac cggcgtgcgt tggctcggta
     3121 aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg
     3181 tagtttcgtg ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg
     3241 caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca
     3301 acccaagcgc gctgtcatta cgggcaatca tcaccggtcg gccttcgtac caacgagagt
     3361 gcggattacg atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc
     3421 cagccacgcc aaatggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct
     3481 gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg
     3541 caagagcttc ctcaagcatc gcaatataat cttcgccgct ctgtaaaagc cgtttttcaa
     3601 tatcagtaaa atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg
     3661 ccgcagctaa ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc
     3721 agagactgtc gcgcaaagat gccgcttctg tgccagttcc tgccggaacg tgagttcccg
     3781 tcaggcggct tagctgcccg gcacgctcgg cggtaaagcc ggcattggca taagcgcaga
     3841 tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga
     3901 tcactcgcgc atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca
     3961 ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta
     4021 aacgctggct acccggctgc gcacccagca atcggtgcaa agtactggca tcttccggaa
     4081 tacgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg
     4141 ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt
     4201 cggccatttg aattaacgct gccagcaact tcgctacggt ggtcgtttta ccggtgccag
     4261 ggccgccgga aatcaccgag atccgccgcg tcagcgccac tgctgccgcc actttttgcc
     4321 agttaatttc atcaccagtc ggaaagagtt tatccagggt ttgcgccaac aaggcttcat
     4381 ccacctcgat ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc
     4441 acatgcgatt caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc
     4501 ggctgaccgc ttgtgaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac
     4561 tgacacaggt cgccaacagc ggatgcgact cctcattatt ttccagtcgt gaaagcggca
     4621 aacaaacgtg cccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag
     4681 gatgttcatc tcccgccacg gtcagggcaa actgcacgtc cagcgggcgt agctgtttgt
     4741 gctccacagc ttccagtaat tgcttttgca atttcattac gcctcctcca gagtcatacc
     4801 ggcaaacatc tcatccatca ggtcaatcaa cccggcgttg gggcgggttg tgtaaatccc
     4861 ttgttgcgga tgttctttat caacaccacg caggaacaga taaataacgc cgccaaagtg
     4921 gcgctcatag tcgtaatcag caatgcgatg gcgcagataa cggtgcagcg ccagggtata
     4981 aagctgatat tgcagatcat agcggtgtgc ctgcattgcc gctgccatag cctgttgggt
     5041 gtaagccgaa ctatcttcac ccaaccagtt ggatttatag tcgagcaggt aataacgccc
     5101 ttcgtggcgg aacaccaggt cgataaagcc ttttaacatg ccacgtgcct gcatgaactc
     5161 cagcggcggg cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct
     5221 ggcgatgagc ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcacggtc
     5281 ggaaagctga ctcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat
     5341 ccactcggtc aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg
     5401 tacccagttc gggtcaaccg gctgggtaaa attgaggtct tcaaacaaac tgtgcaagaa
     5461 tgtccccggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac
     5521 gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg
     5581 accacgctgc tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg
     5641 tagcgtcctc gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc
     5701 accagtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg
     5761 aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgaggg cactttggtg
     5821 gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact
     5881 gcaatgccaa accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg
     5941 ttcgacctcc gcaagatcga cgctttctgg cgcagcgtta agatccagaa cagcctcaaa
     6001 cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa
     6061 ccagaccagc ggatattcca gcccttttga tttatggatc gtgacaatct gtaccagatg
     6121 tttatcactt tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg
     6181 ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgtag
     6241 cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa
     6301 gttttctgca atgttacgcg ccgacatcag cgcccgcatc atcggcataa cgccacgttt
     6361 gcgccagatt tgccgataac catcgaactc ttcgaccacc gcgtcccacg catgttcgtc
     6421 attattcagc gtttcgatat ccagcgcgtt cagccccatc attgacgttg ccagcgcact
     6481 acgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg
     6541 cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag
     6601 caacgttaag gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacactgat
     6661 gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg
     6721 tccggcctgt agccagtcgc gaatttgcgc agcacttacc tgcgccatgg tactttgata
     6781 gtcgccaacg ccgcaacttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc
     6841 acctttaaag acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg
     6901 tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac
     6961 cattccaggt gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg
     7021 cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc
     7081 gccaatcagc aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta
     7141 ctgctggggg tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat
     7201 cgccgctgcc aacacctcac cgctttcgct acgcagcgcg gaatcgagcc gacttaacat
     7261 gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc
     7321 agccaatgcg cgggtgatca ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc
     7381 gatcgcctca aacagtggat gtcgcggggt ttcccccccg gccttcgtgc gatcttctaa
     7441 gaaacgctgg gagaattttt ccagcgactc cggcaactga taactgtttc tctcttcttc
     7501 tgcccaggcg ctgatcttgt cgatccattt agcctgattg ctacggttaa acttgcgtcg
     7561 atcaatacca gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg
     7621 ttttaccgta tcaatacgcg ccacaatttg cgcgtgacgg gaagccagcg tttcatcatc
     7681 gggcggcggt gctttgataa ccggcgcttc accttgcaga taacgattaa tatcgcgcag
     7741 caacgcctgc ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg
     7801 gtagcagtgg cgacgccaga aatcggcgca ggcctggtaa cgtagcagag actcatcttc
     7861 aatcagctgc tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg
     7921 gcaaaaaccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa
     7981 ccacttcgcg gcttgcgctt tatcgtcgat ctcttccagc agacgttcgt acagtggatt
     8041 gtcggtggtt tcacgcagac aggcgatgcg caactcatgg atattgctac ggatacgacc
     8101 acgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag
     8161 cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc
     8221 aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc cctgaaaggg
     8281 caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca
     8341 ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcagga tgcacatatt
     8401 cggctttccc gttctggctg ccggaaatct gcgacagaat agccatgcct tgcggctcga
     8461 ccaccgcctg atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt atctgggcca
     8521 cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttca
     8581 atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga
     8641 tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg
     8701 ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca
     8761 tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt
     8821 cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg
     8881 ctgagcttgt gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg
     8941 tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt
     9001 ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg
     9061 ttgcctgggc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta
     9121 aggcgtcacg ataagccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt
     9181 catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa
     9241 acgctttacc cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa
     9301 gctgatcttc cgtagcggta tagctaaagt atccctcgag caatgcctgg aacagctgcg
     9361 gcagacgctg ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa
     9421 aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat
     9481 aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca
     9541 aaatcaggct gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca
     9601 cgcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa
     9661 tcagcgagaa atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg
     9721 cagctttttg ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg
     9781 catcgacaaa ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg
     9841 cattctgcgg cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg
     9901 caatattgac tgcatccagc gtatgctcaa caggaacgcg aatcatggta tctgccagcc
     9961 attcgacgta atccatatca cgggtgattg acggataacg gaagtcgata tccagcacat
    10021 tcgccagttc atcgaagtat tgtttatcga tccctttttc acgtaacaaa ttgaggtagc
    10081 taaaaatagc cgccacaacc tgatcgcgat tcgccagacc tttatcggtt aaagacgcag
    10141 agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct
    10201 caactaaccc ctgcttttgc agccagtcag acagtgtacc tgggctgcga ttaccaatca
    10261 gataggtaat caattcatcc gttttactgc ggaacttcgc tgagttgtta tcgatgcgaa
    10321 actcaacgcg cagaacttta cgcggcagcg ctgggacgta atgaatgata atgccctttt
    10381 gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc
    10441 gaccaaaagt gtccgccgcc atttttgcca actccggcag cggtttatta ctgtaaataa
    10501 ccgccttcat caaattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca
    10561 ccggattacc aggtttgtcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg
    10621 ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgtgtac
    10681 gcgccatggt taattcagcg ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga
    10741 gcaagggttc tgcaatagca tcggccaggc ggtctaccgc accgggcaag gcgtcgttct
    10801 caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt
    10861 gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac
    10921 tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa
    10981 cgggcaccac cagcgccgat agcgatttaa ctgcctgcgg atcagaaacc agcaagacca
    11041 ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca cttttacgga
    11101 tggtttcctg aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa
    11161 ctaacaacaa taatgctttg aaccaggtgc tgcggggcat tcacggacct cataatcaac
    11221 tttattttct ggccagattc aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt
    11281 gctgcacgag tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa
    11341 gtaagcggat agattgcgca atttttatac agcgctcatg actgattaaa gcgaaacagc
    11401 ggtaacagga aacgttgcga ctgttcaacg attgcctcca ttgtctctgg tgttaattgc
    11461 cgccagagcc tttgatacca gatatcatca ccttcgccac tcaccatcat gttgccttcg
    11521 taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca
    11581 tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc
    11641 aatggcgcgg acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc
    11701 tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt
    11761 tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc
    11821 gccacactta ataaagaggg acgccagcgc aacaagccat ccggctgcac ctgcggcaac
    11881 cagccagtta tctgcacacc gttgcaagcg agatcaattt ccatactctg ccccggctgg
    11941 cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa
    12001 atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt
    12061 tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta
    12121 agtccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc
    12181 acctgcaaac gcatctggaa gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt
    12241 tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct
    12301 ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc
    12361 tggtagtttt gcggatcaaa cggcatccgg gtatggaggc aagtaagatg cgcttttacc
    12421 cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg
    12481 atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg
    12541 gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac
    12601 aggtagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg
    12661 tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa
    12721 accactttga acggaattga acgcattggc atcagagtac aaatgttaac cggcccggcg
    12781 agaaaacgct ggctgatacg ttcttgatcc aggcgctgtg ccagttcatc acgcaatagt
    12841 gacagcggca ccgcatcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat
    12901 tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gagggcgttg
    12961 agcatatcgc gacaaaccgg caaccactct tccagcggac gctcctgtgc cagcccgcga
    13021 cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag
    13081 ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg
    13141 tagcccaaca acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt
    13201 tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac
    13261 tgacgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca
    13321 tccagtaatg ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg
    13381 ataaacgctt caagtacagg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc
    13441 aggtaacgat ccgcaggtgc gctaccaaac acagcctgaa taaacggact gtagctgtcg
    13501 atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc
    13561 gccagcaggc gatcgtgtaa aacttcaact tcacgctgcg ggctatggca aacgtggaag
    13621 gtgatactgc tatccagtgg atcaagcgga cgtttgttat cgctacggga aaactcttcg
    13681 atgttcacac cagcaacggc gcggttttcc agttccagaa tgtcagactg aatgttatgc
    13741 agcaggttat ctggcgtcac atcgacaaag gcgtccagct cctggctgct ctccaggtca
    13801 gaaaggagat aaatgtagtc gcgcccaagc ttgccccatg aggccagcag cgggttgccg
    13861 acatcctgtt caccatcgct gttaaagagc tgcccggcat tttcgctgtc gcgaaatagc
    13921 ggtaattcgc gatcttcaaa actgtgtcgg cgctggcgag tcagtagttt cgccagataa
    13981 gcaggatctt taatgtcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt
    14041 tcaatatgtt tacccagcgc ctggagcgcc tggagataaa caggcggtaa cgcggaaata
    14101 ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt
    14161 tcgataaagc gctgatagag attggcgcgg tgccagcgcg gttgtccgag ttcatgggta
    14221 tattccacca gcgccttcca caacggcgct tgccaggcct gtgcctctcc cagcccttca
    14281 accaaatgtc ctgtttccca ctgtgccagc cagtccggac gatagaccag atactggtca
    14341 aacaggtccg ccgcttttga ggaaagctgg aacagctttc gcttgtcgct atcgtcagtc
    14401 agataatgcc gcaacagggt aaagtcttcg cgctccagca attgcggcag cagagtcatc
    14461 agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatctc cggtaacacc
    14521 cggacgaaca tatcccagat aaagctcgca ggcagcggaa aatcaatgtt tgccgcaata
    14581 ccaaactttt gcgacagggt catttgcagc cactgtgcca taccggtact ttgcaccaga
    14641 atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac
    14701 gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac
    14761 tgacgattcg ggcaatgcag gcgggtcatc tcgcctgctc tgcccccggg tgaaactagc
    14821 gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg
    14881 gcaggtggcg aaatcgcgcg caattgcgtt tgctgccaac catgccgcca gagctgttgg
    14941 tactggtttc tgctggtaag actgttcatt aatgttcgct ggaaacccga taacgcagtg
    15001 acaatcatca ccatcaacac catcgccaac attacctccg gcaggctaaa gccttgttga
    15061 ttcttcaggg aagctgacat aacgccccct ctttcagcgg acaaaaatcg ctccagccgc
    15121 gtggcgaaaa aacaattttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt
    15181 cataaccggc aattaataag gcttcattat cttccagtaa acgcaaacaa acccgggcac
    15241 cagtggcagc gtacagtaag cactgaactg cgggctgcgt ctgccaggag tgcattttcc
    15301 cccacgccaa cgccgactga acgatggcct ggcggcgcag tgactgactt tccatgctca
    15361 cgcgagaggc aaaactgcga tcctgctggc tcattccttg taatagcaag ctacccaaaa
    15421 ccagcagcat caggaccaga gccagtgacg aaacaccctt ttcgcggttc acaggttgaa
    15481 tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg
    15541 catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt
    15601 gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt
    15661 cgcaccgcgt agcgtttcca acacttgctc cttcagacga aatccaatct ggtcggactc
    15721 ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca
    15781 gtcaccctgt ccgacaattt ccagcccttc gccggtacag atgccatgac aataacccgc
    15841 cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagtgccag
    15901 cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccaa
    15961 caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc
    16021 ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa
    16081 accaccagcc accactcgcc cgttgagttt ttgaagccaa tatgcccggc ccatgcggta
    16141 ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag
    16201 cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tcgccccagc aacggaactc
    16261 acaaggcacc ataacgtccc ctccctgata aaactgatac tgtggtcgcg gttatgccag
    16321 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccaaagc
    16381 cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa
    16441 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
    16501 gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
    16561 gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa
    16621 cggagggtaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    16681 atagccaccg gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg
    16741 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    16801 ggttcacggc ttaattgcag atgagtttga tccatatgat tactgtacag gtgcgtatcg
    16861 ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc
    16921 agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga
    16981 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt
    17041 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgggaa
    17101 tccgggtcgt ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatct
    17161 ggcgttggcc aggcgcgcca ctgtttacca tacactggtc cgaggtcgcc gttttcatcg
    17221 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc
    17281 tgcagaaacc acagcagttc atggatgatg gaacgcaggt ggcaacgttt agttgtcacc
    17341 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    17401 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    17461 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    17521 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac
    17581 tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    17641 aacgcgccgt agccaatcag gaacaagcca gacacggctc ccatcgggcg cggtttacga
    17701 atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt
    17761 gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt
    17821 tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc
    17881 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccaaacgccc ggcaccaaga
    17941 ccaaacggaa tgagtggtgc gataaaatca gaaacctgga agaaggaacg tttagtacgg
    18001 cgggcaaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg
    18061 ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac
    18121 agaacataac caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt
    18181 tcaacttcat ttttggtcca gccgctgccc ggacgattcg cccgtcgtgt tgccagccac
    18241 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    18301 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    18361 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    18421 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    18481 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga
    18541 cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta
    18601 cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc
    18661 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    18721 cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct
    18781 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc
    18841 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    18901 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact
    18961 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    19021 cgtccggcac gttcaatcag gcggcgtgct tcgtcaactt catcgaggct tgtgaccatc
    19081 ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg
    19141 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    19201 ttctcttcgc taatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    19261 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    19321 tcttccgacg gaaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    19381 ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    19441 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    19501 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    19561 ggatcgacca gcaattcacc gcgatagcca tcgacgatca gcgtccgacg atgcagcacc
    19621 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    19681 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    19741 tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg
    19801 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    19861 tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    19921 tcaatgaccg tttttaccgc ccattctgcc accgagcctt tatcaacctc ggcaaacaat
    19981 tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt
    20041 tctttttgtg caccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    20101 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    20161 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc tacaccaggt
    20221 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc
    20281 gactgagaaa gaatcgctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    20341 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    20401 tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat
    20461 ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    20521 aggccgacga tcccttcatc aaaggcgagc gttacagtgc gaccgcgtgg ttttttcagc
    20581 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    20641 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    20701 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    20761 acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc
    20821 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    20881 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    20941 gtactgctgg tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat
    21001 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    21061 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    21121 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg
    21181 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    21241 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    21301 cctcaaaata aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca
    21361 ctctctgcag ggcattacac actaacaaca attgaataac tcacagttat gtgcagagtt
    21421 ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg
    21481 aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca
    21541 ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttaagtaatg aaaaagagta
    21601 attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    21661 tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc
    21721 gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc
    21781 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacccgtt
    21841 gcagtgttgc gcaactcgat taccggcaac ctaaaaagca atataaatca gataattgaa
    21901 caacgcatgt ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt
    21961 aaccaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag
    22021 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    22081 ttgccgggct ggttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac
    22141 tggagatctg gctaggtgcc agcgcaggga gtaaacctga acaagatttt gctgctctgg
    22201 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    22261 tttgtgttgc cccgttaacg ggcaatagcg gagtgacctg ggaaaccagc cacgtgcgcc
    22321 acaaactcaa acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc
    22381 agcgtcgcgt tggatcaccg ttgctgtgga gcccgaatga agaggaagac cggcagctac
    22441 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggattaccg
    22501 ctcgtcacgg ggagtattta cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg
    22561 aagccattgg tgcacggggc gaacggattc tgacgctgcc gcgcggcttt tatttgaaga
    22621 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac
    22681 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    22741 atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga
    22801 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agctccccac
    22861 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    22921 gctgctggca tcaatcgcct gggttacgcg cctgacgaat ccgcttttta caatattcag
    22981 tcaggaaatt tctgcccgtg acttgattct gcttctgggt ggcttgttcc ttatctggaa
    23041 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    23101 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga
    23161 ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg ccgccgtggt
    23221 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgagcgcca
    23281 tccttcggta aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct
    23341 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    23401 tgcggttgaa agcctcaacc tgattcgcaa caaaaagaac ccgctctgat actccgtacg
    23461 ctctcctgcg ggagggcgta ctcacttctg ttaccctcac agtttaagat tttcctgctt
    23521 tcaagatata taacgtcagt ttataaacag actattatca taggtgggcc aaatgaggat
    23581 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    23641 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    23701 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    23761 atgcaaatta accgtgatga tgtttcgcaa ataattgaac gttaacaaat aaggtcagca
    23821 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
    23881 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
    23941 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
    24001 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
    24061 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
    24121 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
    24181 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
    24241 tccgcgaagc gctgcatgac agcctcaagc gtctgcagac tgattacctc gatctttatc
    24301 aggtgcactg gccgcagcgc ccaaccaact gcttcggcaa actcggttat agctggacag
    24361 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgag
    24421 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
    24481 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
    24541 tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac
    24601 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
    24661 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
    24721 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gcccctgctc
    24781 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
    24841 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
    24901 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
    24961 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    25021 tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
    25081 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    25141 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    25201 gagcggaacg acaaaaaagc cccccaacac gccaatcagc atcaacaagg cataggctgg
    25261 cagctgctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa ttccggctgg
    25321 catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac
    25381 gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag
    25441 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    25501 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
    25561 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    25621 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    25681 ggcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
    25741 cgctatggta gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    25801 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    25861 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    25921 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    25981 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    26041 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
    26101 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    26161 gtgcactgac tcactcatcg tgttgttcca cttcgtctac ccagcttttc aacgtgacaa
    26221 agtcaggttt gccgctgcca agtaatggca tctgtttcaa atagcgaata tcacgcggta
    26281 cagcaagctc cggcacgccg tgctcacggg catactgttg caacttatcg cgcgtcagtt
    26341 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    26401 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    26461 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    26521 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    26581 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    26641 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    26701 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    26761 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    26821 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    26881 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    26941 tcgaagtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
    27001 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
    27061 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
    27121 tgaagtcggc aatcgtttta atctgctcga cattcgccag aatgctttta tggctatgga
    27181 cgacgccttt tggatggcct tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg
    27241 gctgctgttt aacctgtgcc agacgcggca tcagcaaatg agcgaagatc catactttgt
    27301 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    27361 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    27421 tgatttcagc cgccgtaata gcactggtca gcccttttac cccagcagtg tagttcatca
    27481 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    27541 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    27601 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaaattg acgtcttcga
    27661 cacatttctt ccctgctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
    27721 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    27781 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
    27841 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    27901 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    27961 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
    28021 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
    28081 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    28141 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    28201 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    28261 cctgggggtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    28321 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    28381 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    28441 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    28501 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    28561 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    28621 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    28681 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    28741 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
    28801 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
    28861 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
    28921 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
    28981 taataattca cccaaagcca gcgaagcttc ccggctggct gtgcatagtg caatggagtc
    29041 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
    29101 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
    29161 acaggtggct tatcacaccg gtaatttctt attgattggc aatggttacc acaacgaaca
    29221 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
    29281 tgccaaaatg atcccggatg ccgatttagc ctcattattg aaacaaatgc ccggtatggt
    29341 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctctgg acgatcgtta
    29401 cggtgcctgg ctggctacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
    29461 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
    29521 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag
    29581 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
    29641 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
    29701 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
    29761 tccgcgcctg accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact
    29821 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
    29881 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
    29941 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
    30001 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt
    30061 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacctg
    30121 ccttcacttc cggcaaggcg cggggttcaa catttccccc ttcctgctgg gtaaagacat
    30181 cgcccgattc acataacggt ccggcgacga cggtttccac cgttggtgcg tgttccagag
    30241 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
    30301 tcagatcgtt aaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
    30361 tccgcacctg agtaattaac acgccagact gcgctaccag gaagcgaccc ggttcaattt
    30421 ccagtttcac agggtggccc aaatggcggg cgatttgctc acgcgcggca ttccacagac
    30481 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
    30541 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgc accatagcac
    30601 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
    30661 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
    30721 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
    30781 ccggattaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgaaca
    30841 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
    30901 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
    30961 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
    31021 gcattaagcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
    31081 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
    31141 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
    31201 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
    31261 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg
    31321 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
    31381 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
    31441 tcagccgcga actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac
    31501 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
    31561 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
    31621 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
    31681 cctttctggt acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
    31741 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
    31801 ctgcgggaac agaacgtacc gaattactct ctttagatga attgtgtgtg ttacctccgg
    31861 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
    31921 tcagcctttc ccatactgac agctatcgcc agttgctgga tcagctattt actgaacatc
    31981 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
    32041 gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg
    32101 gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
    32161 tgcaccgccc gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcggggttac
    32221 cgaaactggt cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac
    32281 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
    32341 caccagtaaa ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag
    32401 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
    32461 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
    32521 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
    32581 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
    32641 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
    32701 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
    32761 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
    32821 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
    32881 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
    32941 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
    33001 gacaaacgcc tcaacggcca ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac
    33061 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
    33121 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg
    33181 cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
    33241 tcaagcagtg tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtc
    33301 gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
    33361 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
    33421 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
    33481 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct
    33541 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
    33601 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgca
    33661 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
    33721 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ttgttttaac
    33781 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    33841 atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta
    33901 tttggcagga agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac
    33961 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    34021 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    34081 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    34141 acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    34201 acaaacagga tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa
    34261 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    34321 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    34381 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    34441 cgccgcgtat cacgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    34501 ttttcctgcc agcagagagt aagacatagt gaaaaaatac gtgaacaatt cacacaggtg
    34561 tcaggtcgga aacagcataa atatggatta aattgctgcg
//
LOCUS       DAZKFZ010000029        33648 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000029 DAZKFZ010000000
VERSION     DAZKFZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            25..261
                     /gene="ydhI"
                     /locus_tag="ACHOQW_001310"
     CDS             25..261
                     /gene="ydhI"
                     /locus_tag="ACHOQW_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP9572410.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            264..1121
                     /gene="ydhJ"
                     /locus_tag="ACHOQW_001311"
     CDS             264..1121
                     /gene="ydhJ"
                     /locus_tag="ACHOQW_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9572411.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1121..3133
                     /gene="ydhK"
                     /locus_tag="ACHOQW_001312"
     CDS             1121..3133
                     /gene="ydhK"
                     /locus_tag="ACHOQW_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9572412.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3134..3655)
                     /gene="sodC"
                     /locus_tag="ACHOQW_001313"
     CDS             complement(3134..3655)
                     /gene="sodC"
                     /locus_tag="ACHOQW_001313"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9572413.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSLGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3736..4632)
                     /gene="ydhF"
                     /locus_tag="ACHOQW_001314"
                     /pseudo
     CDS             complement(3736..4632)
                     /gene="ydhF"
                     /locus_tag="ACHOQW_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            complement(4681..4920)
                     /gene="ydhL"
                     /locus_tag="ACHOQW_001315"
     CDS             complement(4681..4920)
                     /gene="ydhL"
                     /locus_tag="ACHOQW_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP9572414.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5023..5622
                     /gene="nemR"
                     /locus_tag="ACHOQW_001316"
     CDS             5023..5622
                     /gene="nemR"
                     /locus_tag="ACHOQW_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP9572415.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            5659..6756
                     /gene="nemA"
                     /locus_tag="ACHOQW_001317"
     CDS             5659..6756
                     /gene="nemA"
                     /locus_tag="ACHOQW_001317"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP9572416.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6837..7244
                     /gene="gloA"
                     /locus_tag="ACHOQW_001318"
     CDS             6837..7244
                     /gene="gloA"
                     /locus_tag="ACHOQW_001318"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9572417.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7347..7994
                     /gene="rnt"
                     /locus_tag="ACHOQW_001319"
     CDS             7347..7994
                     /gene="rnt"
                     /locus_tag="ACHOQW_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP9572418.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8087..12703
                     /gene="lhr"
                     /locus_tag="ACHOQW_001320"
     CDS             8087..12703
                     /gene="lhr"
                     /locus_tag="ACHOQW_001320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9572419.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12754..13101)
                     /gene="grxD"
                     /locus_tag="ACHOQW_001321"
     CDS             complement(12754..13101)
                     /gene="grxD"
                     /locus_tag="ACHOQW_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP9572420.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13436..14251
                     /gene="mepH"
                     /locus_tag="ACHOQW_001322"
     CDS             13436..14251
                     /gene="mepH"
                     /locus_tag="ACHOQW_001322"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP9572421.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14379..14960
                     /gene="sodB"
                     /locus_tag="ACHOQW_001323"
     CDS             14379..14960
                     /gene="sodB"
                     /locus_tag="ACHOQW_001323"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP9572422.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15106..16275)
                     /gene="ydhP"
                     /locus_tag="ACHOQW_001324"
     CDS             complement(15106..16275)
                     /gene="ydhP"
                     /locus_tag="ACHOQW_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572423.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16441..16530)
                     /gene="cydH"
                     /locus_tag="ACHOQW_001325"
                     /gene_synonym="ynhF"
     CDS             complement(16441..16530)
                     /gene="cydH"
                     /locus_tag="ACHOQW_001325"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP9572424.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16829..17854
                     /gene="purR"
                     /locus_tag="ACHOQW_001326"
     CDS             16829..17854
                     /gene="purR"
                     /locus_tag="ACHOQW_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP9572425.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17851..18783)
                     /gene="punR"
                     /locus_tag="ACHOQW_001327"
     CDS             complement(17851..18783)
                     /gene="punR"
                     /locus_tag="ACHOQW_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP9572426.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18896..20107
                     /gene="punC"
                     /locus_tag="ACHOQW_001328"
     CDS             18896..20107
                     /gene="punC"
                     /locus_tag="ACHOQW_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP9572427.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20398..21546
                     /gene="cfa"
                     /locus_tag="ACHOQW_001329"
     CDS             20398..21546
                     /gene="cfa"
                     /locus_tag="ACHOQW_001329"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP9572428.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21586..22227)
                     /gene="ribE"
                     /locus_tag="ACHOQW_001330"
     CDS             complement(21586..22227)
                     /gene="ribE"
                     /locus_tag="ACHOQW_001330"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP9572429.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22442..23815
                     /gene="mdtK"
                     /locus_tag="ACHOQW_001331"
     CDS             22442..23815
                     /gene="mdtK"
                     /locus_tag="ACHOQW_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP9572430.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            complement(23856..25112)
                     /gene="ydhQ"
                     /locus_tag="ACHOQW_001332"
     CDS             complement(23856..25112)
                     /gene="ydhQ"
                     /locus_tag="ACHOQW_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9572431.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25420..25496
                     /locus_tag="ACHOQW_001333"
     tRNA            25420..25496
                     /locus_tag="ACHOQW_001333"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25454..25456,aa:Val,seq:gac)
     gene            25501..25577
                     /locus_tag="ACHOQW_001334"
     tRNA            25501..25577
                     /locus_tag="ACHOQW_001334"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25535..25537,aa:Val,seq:gac)
     gene            25685..25990
                     /gene="ydhR"
                     /locus_tag="ACHOQW_001335"
     CDS             25685..25990
                     /gene="ydhR"
                     /locus_tag="ACHOQW_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP9572432.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26116..27720
                     /gene="ydhS"
                     /locus_tag="ACHOQW_001336"
     CDS             26116..27720
                     /gene="ydhS"
                     /locus_tag="ACHOQW_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP9572433.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27732..28544)
                     /gene="ydhT"
                     /locus_tag="ACHOQW_001337"
     CDS             complement(27732..28544)
                     /gene="ydhT"
                     /locus_tag="ACHOQW_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP9572434.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(28548..29333)
                     /gene="phsC"
                     /locus_tag="ACHOQW_001338"
     CDS             complement(28548..29333)
                     /gene="phsC"
                     /locus_tag="ACHOQW_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP9572435.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29330..29998)
                     /gene="ydhX"
                     /locus_tag="ACHOQW_001339"
     CDS             complement(29330..29998)
                     /gene="ydhX"
                     /locus_tag="ACHOQW_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9572436.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30062..30700)
                     /gene="ydhW"
                     /locus_tag="ACHOQW_001340"
     CDS             complement(30062..30700)
                     /gene="ydhW"
                     /locus_tag="ACHOQW_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP9572437.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30713..32815)
                     /gene="ydhV"
                     /locus_tag="ACHOQW_001341"
     CDS             complement(30713..32815)
                     /gene="ydhV"
                     /locus_tag="ACHOQW_001341"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP9572438.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(32836..33462)
                     /gene="ydhY"
                     /locus_tag="ACHOQW_001342"
     CDS             complement(32836..33462)
                     /gene="ydhY"
                     /locus_tag="ACHOQW_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP9572439.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8078 a         8875 c         8299 g         8396 t
ORIGIN      
        1 ttcggtcgta aaggaatcta ctttgtgaag tttatgctca atgcaacagg attgccctta
       61 caagatctgg tgttcggtgc gtccgtctac tttcctccgt ttttcaaagc cttcgcgttt
      121 ggattcgtca tctggcttgt cgtacaccgc ctgcttcgtg gctggatcta cgccggtgac
      181 atctggcatc ccttgttaat ggatttatcg ctgtttgcga tttgcgtttg ccttgctctg
      241 gcaatactga ttgcgtggta actatgtcaa ttaaaacaat taagtatttc tcaacaatca
      301 ttgtagcggt agttgcggtt cttgccggat ggtggctgtg gaattattac atgcaatctc
      361 cgtggacacg cgacgggaaa atacgcgcag aacaggtttc tatcacccca caggtgtcgg
      421 gacgtattgt tgagctgaat ataaaagaca accagctggt taacgcgggg gatcttctgc
      481 tcacgatcga taaaacgccc tttcagatcg ccgagctgaa tgcacaggct cagctggcga
      541 aagcgcaatc tgacctcgca aaagccaata acgaagcgaa tcgtcgtcgt catctctcac
      601 aaaattttat ttctgccgaa gaactcgata ccgctaacct caatgttaaa gcgatgcagg
      661 ccagtgttga tgccgcacag gcgacgctga aacagacaca atggcaactg gcgcaaacgg
      721 aaattcgcgc cccggtgagt ggatgggtga ctaatctcac tacccgcatc ggcgattacg
      781 ctgacaccgg gaaaccgctg tttgcccttg tcgacagcca ctcgttttac gtcattggtt
      841 attttgaaga aaccaagttg cgccatatcc gcgaaggtgc accagcacaa attaccctct
      901 atagtgataa caaaacgtta cagggtcacg tttccagtat cggtcgggcg atttatgatc
      961 agagcgttga aagtgactca agccttattc cggatgtgaa acctaacgtc ccctgggttc
     1021 gtctcgccca acgcgttccc gtccgctttg cgctggataa agttccaggc gatgtcacgc
     1081 tggtgtcggg caccacctgt agcattgccg taggtcaata atgaacgcat cgtcatggtc
     1141 cttgcgcaat ttgccctggt tcagggccac gctggcgcaa tggcgttatg cgttacgcaa
     1201 taccattgcc atgtgtctgg cgctgacggt tgcctattat ttgaatctgg atgaacccta
     1261 ttgggcgatg acctcggctg cagtggttag ctttcccacc gttggcggtg tcatcagcaa
     1321 aagcctcgga cgcatcgctg gcagtttact gggagccatt gcggcactgc ttcttgccgg
     1381 gcatacgctc aatgagccgt ggttttttct attgagcatg tcggcgtggc ttggcttttg
     1441 tacctgggcc tgtgcgcact tcacgaataa cgtcgcgtat gcatttcaac tggcgggcta
     1501 cacggctgcc atcatcgcct ttccgatggt taatattact gaggccagcc agctgtggga
     1561 tatcgctcag gcgcgcgttt gcgaggtgat tgtcggtatt ttgtgcggcg gcatgatgat
     1621 gatgatcctg cctagcagtt ccgatgctac agccctttta accgcattga aaaacatgca
     1681 cgcccgatta ctggaacatg ccagtttact ctggcagcct gaaacaaccg atgccattcg
     1741 tgcagcacat gaaggggtga ttgggcagat actgaccatg aatttgctgc gtatccaggc
     1801 tttctggagc cactatcgtt ttcgccagca aaacgcgcgc cttaatgcgc tgctccacca
     1861 gcaattacgt atgaccagtg tcatctccag cctgcgacgt atgttgctca actggccctc
     1921 accgccaggt gccacgcgag aaattctcga acagttgctg acggcgctcg ccagttcgca
     1981 aacagatgtt tacaccgtcg cacgtattat cgccccgcta cgcccgacca acgtcgccga
     2041 ctatcggcac gtcgccttct ggcagcgact acgttatttt tgccgccttt atctgcaaag
     2101 tagtcaggaa ttacatcgtc tgcaaagcga tgtagatgat catgccagac tcccacggac
     2161 atccggcctg gctcgtcata ccgataacgc cgaagctatg tggagcggac tgcgtacatt
     2221 ttgtacgttg atgatgattg gcgcatggag tattgcttcg caatgggatg ccggtgccaa
     2281 tgcattaacg ctggcagcga ttagctgcgt actctactcc gccgtcgcag caccgtttaa
     2341 gtcgttgtca ctgctgatgc gcacgctggt gttactttcg ctattcagct ttgtggtcaa
     2401 atttggtctg atggtccaga ttagcgatct gtggcaattt ttactgtttc tctttccact
     2461 gctggcgaca atgcagcttc ttaaattgca gatgcccaaa tttgccgcat tgtgggggca
     2521 actgattgtt tttatgggtt cttttatcgc cgtcactaat cccccggtgt ataattttgc
     2581 tgattttctt aacgataatc tggcaaaaat cgttggcgtc gcgttggcgt ggttagcgtt
     2641 cgccattctg cgtccaggat cggatgctcg caaaagccgc cgccatattc gcgcgctgcg
     2701 ccgggatttt gtcgatcaac taagccgcca tccaacactg agtgaaagcg aatttgaatc
     2761 gctcacttat catcacgtca gtcagttgag taacagccag gatgcgctgg ctcgccgttg
     2821 gttattacgc tggggtatag tgctgctgaa ctgttctcat gttgtctggc aattacgcga
     2881 ctgggaatcg cgttccgatc cgttatcgcg agtacgggat aactgtattt cactgttgcg
     2941 gggagtgatg agtgagcgtg gcgttcagca aaaatcactg gcggccacac ttgaagaatt
     3001 acagcggatt tgcgacagcc ttgcccgcca tcatcaacct gccgcccgtg agctggcggc
     3061 aattgtctgg cggctgtact gctcgctttc gcaacttgag caagcaccac cgcaaggtac
     3121 gctggcctct taattactta attacaccac aggcatagcg ttcaccgcca ccgcccagcg
     3181 gtttaggttg atcggacata ttatcgccgc caacgtggac catcagcgct ttgtctttga
     3241 tttcatccag tgatttcaga cgaggcgcga tgacggcatc ggtagctttg ccgtcattat
     3301 tgacgaccag tgcaggcaga tcgcctaaat gcccggcacc ttctggccct tcatgtttac
     3361 cggtattttg tggatcaaga tgcccgcctg cggattccgc ggcgctggct ttgccatctt
     3421 tggtggctgg ctggcagctt cctttggcat gaatatggaa gccatgttca ccgggaggta
     3481 atgctttcag atcgggcgaa aactccagac ctttatcggt ttcagtaatg gtgacgctac
     3541 caattgactg cccaacccct agcgacgtga cgaggttcat ctcgactttt tcactggcag
     3601 cttgtgcgcc ggtcgcaaca accagcgcca gaatggccag actaaaacgt ttcataggac
     3661 ctccgttcat tgtgcattcc tgaataagtg taaaccagtg acacattttt gaacggcggc
     3721 tattcctaaa agtgcttacg gtacgtcgta ccccagtgcc gctttacgga tacgaaacca
     3781 ttgttgacgg gtcattttca gtgtttctgc ttcgacagct gcccgtacgc gctcaatttt
     3841 acctcagccg ataattggca gcggctgcga cggtaaacgt aatacccagg cgtaaaccac
     3901 ctgttcaatc gagcccgcgt ttaactcctc cgccaccaca gccagttcat cacgcagcgg
     3961 ctggaaataa tcatcattaa acagacgacc accaccaagg caggaccacg ccatcggacg
     4021 aacacgcagt tgttgcagtt ggtcgagcgt gccatccagc agtaacggct gatgtaccgg
     4081 ggatatttcc acctgattag tggcaagggt aaacggcaga cgtgattgca acagggcaaa
     4141 ttgcgcaggc gtgaagttgg atacgccaaa atgacgcact ttgccgctct gatgcagatg
     4201 tttgaacgcg tccgccactt catcggcatc cattaacggg tccgggcggt ggattaacag
     4261 cagatccaga tgatcggtcg caagattaat tagcgactgt tcggcgctct taatgatgtg
     4321 atcgcggtca gtgatgtaat gaccaatgac gttttcttca cgcgcggtcg tcgcgatacc
     4381 gcatttactg acgatttcca tccgctcacg caggtgaggt gccagtttca gcgcctcgcc
     4441 aaacgccgct tcgcactgat agccaccata aatatcagca tggtccacgg tggtcacgcc
     4501 gagatccaga tgctcttcaa taaaactgac cagctggcgg gcggacatgt tccagtccat
     4561 caatcgccag tagcccatca caaaacggga aaactccggg ccttgcggcg caatagtaat
     4621 acgctgaacc ataatcgctt cctcttatca gatatgagag gagtatacgc aagattaggc
     4681 tcaaaagagt gatggttgct ccggttcgtc tgatgacgct ggcttatttg cacgtaattt
     4741 gcgcattaat cgctgccgac aaaggcgcag cacctcttgt ttttcgccat cgctcatttt
     4801 attccagtta aaacgctcat cccggcttcg aaaacagccg cgacaaaacc cgcgttcgtc
     4861 agactggcaa attccccggc acgggctctg gacgggaaag aactctaatt gctccgccac
     4921 ttcgccctcc tcaaataaga ttattaccat tattgaagct gttaatgccc aaagtagcaa
     4981 ctttgcttgc actagaccga ctggtctact acactccaac gcatgaacaa acacaccgaa
     5041 catgatactc gcgaacatct cctggcgacg ggcgagcaac tttgcctgca acgtggattc
     5101 accgggatgg ggctaagcga attactaaaa accgctgaag tgccgaaagg gtccttctat
     5161 cactactttc gctctaaaga agcgtttggc gttgccatgc ttgagcgcca ttacgccaca
     5221 tatcaccaac gactgactga gttgctgcaa tccggcgaag gtaactaccg cgaccgcata
     5281 ctggcttatt accagcaaac actgaaccag ttttgccaac atggaaccat cagtggttgc
     5341 ctgacagtaa aactctctgc cgaagtgtgc gatctgtcag aagatatgcg tagcgcgatg
     5401 gataaaggcg ctcgcggcgt gatcgccctg ctctcgcagg ctctggaaaa tggccgtgat
     5461 agccattgtt taaccttttg tggcgaaccg ctgcaacagg cacaagtgct ttacgcacta
     5521 tggctgggtg cgaatctgca ggccaaaatt tcgcgcaatt ccgagccact ggaaaacgcg
     5581 ctggcacatg taaaaaccat tattgcgacg cctgccgttt agcaggcatt ttttatcacc
     5641 agacgaccgg gagcctttat gtcatctgaa aaactgtatt ccccactgaa agtgggcgca
     5701 ataactgcgg caaaccgtat ttttatggca ccgctgacgc gtctgcgcag tattgaaccg
     5761 ggtgacatcc ctaccccgtt gatggcggaa tactatcgcc aacgtgccag tgccggtttg
     5821 attattagcg aagccacgca aatttctgcc caggcaaaag ggtatgctgg tgcacctggc
     5881 atccatagtc cggaacaaat tgccgcatgg aaaaaaatta ccgctggcgt tcatgctgaa
     5941 aatgggcata tggccgtaca gctgtggcac accggacgca tttctcacgc cagcctgcaa
     6001 cctggcggcc aggcaccagt agcgccttca gcacttagcg cgggaacacg tacttctctg
     6061 cgcgatgaaa atggtcaggc gatccgtgtt gaaacatcca tgccgcgtgc gcttgaactg
     6121 gaagagattc caagtatcgt caatgatttc cgtcaggcta ttgctaacgc gcgtgaagcc
     6181 ggttttgatc tggtagagct ccactctgct catggttatt tgctgcatca gttcctttct
     6241 ccttcttcaa accatcgtac cgatcagtac ggcggcagcg tggaaaatcg cgcacgtttg
     6301 gtactggaag tggtcgatgc cgggattgaa gaatggggtg ccgatcgcat tggcattcgc
     6361 gtttcgccaa tcggtacttt ccagaacacg gataacggcc cgaatgaaga agccgatgca
     6421 ctgtatctga ttgaacaact gggtaaacgc ggcattgctt atctgcatat gtcagaacca
     6481 gattgggcgg ggggtgaacc gtatactgat gcgttccgcg aaaaagtacg cgcccgtttc
     6541 cacggtccga ttatcggcgc aggtgcatac acagtagaaa aagctgaaac gctgatcggc
     6601 aaagggttaa ttgatgcggt ggcatttggt cgtgactgga ttgcgaaccc ggatctggtc
     6661 gcccgcttgc agcgcaaagc tgagcttaac ccacagcgtg ctgaaagttt ctatggtggc
     6721 ggtgcggaag gctacactga ttacccgacg ttgtaatcca acattgcgag cggcgtaaag
     6781 ccgccgctat actaaaacaa cattttgaat ctgttagcca ttttgaggat aaaaagatgc
     6841 gtcttcttca taccatgctg cgcgttggcg atctgcaacg ctccatcgat ttttatacca
     6901 aagtgctggg catgaaactg ctgcgtacca gcgaaaaccc ggaatacaaa tactcactgg
     6961 cgtttgttgg ctacggcccg gaaaccgaag aagcggtgat tgaactgacc tacaactggg
     7021 gcgtggataa atacgaactc ggcactgctt atggtcacat cgcgcttagc gtagataatg
     7081 ccgctgaagc gtgcgaaaaa atccgtcaaa acgggggtaa cgtgacccgt gaagcgggtc
     7141 cggtaaaagg cggtactacg gttatcgcgt ttgtggaaga tccggacggt tacaaaattg
     7201 agttaatcga agagaaagac gccggtcgcg gtctgggcaa ctaatctcct gccgggcgcg
     7261 aactcatcgc gcccgcatct ttactgcatc gacaagtaat atttgtcata atgcgcgctg
     7321 caatttatcc gtattaagag aatcagatgt ccgataacgc tcaacttacc ggtctgtgcg
     7381 accgttttcg tggcttttat cctgttgtga tcgatgttga aacagctgga tttaacgcca
     7441 aaaccgatgc gctgcttgag attgccgcca tcaccctgaa aatggatgaa caaggctggc
     7501 tgatgccgga caccacatta catttccacg tcgaaccatt tgtcggcgca aatttgcaac
     7561 cagaagccct tgctttcaac ggcattgacc cgaacgatcc cgatcgcggc gcggtcagcg
     7621 aatacgaggc gctgcacgaa atttttaaag ttgtacgtaa aggtattaaa gcgagcggct
     7681 gtaaccgcgc cattatggtg gcgcacaacg ccaatttcga tcacagcttt atgatggccg
     7741 ccgcagaacg cgcctcactg aaacgtaacc cgttccaccc tttcgccact tttgacactg
     7801 ctgcactggc cgggctggca ctcggacaaa ccgtattgtc aaaggcttgc cagaccgctg
     7861 gcatggactt cgacagcacc caggcgcact ccgcgctgta cgacaccgaa cgcactgcgg
     7921 tgctgttttg tgaaatcgtc aaccgctgga aacgtctggg aggctggccg ctacctgccg
     7981 ccgaagaggt gtaatcgagt cgatgcctga tgacatgcaa tgattcaggc atctatagtg
     8041 aggctattcc acgcatcatg catgatattc acggggaata gcgttaatgg cagataatcc
     8101 aaacccttca tcgcccctgc cggacgtgtt ttcaccggcg acccgcgact ggtttcttcg
     8161 cgcctttaaa cagccgaccg ctgtccagtc gcaaacctgg catgtggcgg cgcgaggcga
     8221 acatgcgctg gtgattgcac cgactggctc cgggaaaacg ctggcagcat ttctctacgc
     8281 cctcgatcga ctcttccgcg aaggcggcga agatacccgc gaggcgcata aacgtaaaac
     8341 ctcacgcatc ctctatattt caccgataaa agccctgggc accgacgttc agcgcaactt
     8401 gcagatcccg ttgaagggta ttgccgatga acggcggcgg cgcggcgaaa cggaagtcaa
     8461 tcttcgcgtg gggatccgta ctggcgatac gcctgcacag gaacgcagca aactcacccg
     8521 taatccgccg gatattctga tcaccacacc cgaatcactc tatctgatgc tgacctcccg
     8581 cgcgcgcgaa acgctacgcg gcgtcgaaac ggtaattatt gatgaagtcc acgcggtagc
     8641 gggaagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
     8701 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
     8761 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccgcaatgc gtcatccgca
     8821 gatacggatt gtcgtaccag tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
     8881 tggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
     8941 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcgc gggggctggc
     9001 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
     9061 tatcgccgct gatgcggccc atttcgagtc gacctctggc gcaacctcta accgtgtaca
     9121 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
     9181 aatcaccgag caggcgctga aatcgggtga gttacgctgc gtggtcgcaa cttccagtct
     9241 cgaactgggg attgatatgg gcgctgtgga gctggtgatt caggtggcaa cgccgctttc
     9301 tgttgccagt gggttacaac gcattggacg tgccggacac cagataggag gtatctccaa
     9361 agggcttttt ttcccccgaa ccaggcgtga cttagtcgat tccgcagtca ttgtagagtg
     9421 tatgttcgca ggcaggctgg aaaacctgac acctccgcat aatccactcg acgtccttgc
     9481 gcagcacacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
     9541 cgtacgccgt gccgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
     9601 tatgctttcc ggacgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
     9661 gaacagggag accgggatat tgaccgcccg acctggcgct cagctgttgg cggttaccag
     9721 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gggaagaaca
     9781 ggccggttcg cgacgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
     9841 cgatattatc actctcggcg caacctcatg gcggatccag caaatcaccc gcgatcaggt
     9901 gattgtgact cctgctccgg gtcgttctgc ccggctcccc ttctggcgtg gtgaaggtaa
     9961 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttgc tggcggatgg
    10021 agcgttcttt tccgggacta ttcccccgtg gctggctgaa gaaaatacga tcgccaatat
    10081 tcaggggttg attgacgagc agcgcaacgc gacgggtatt gttccgggga gtcgtcatct
    10141 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    10201 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgcgctttg
    10261 gggcgctgac gcgtctgtgg tcgccagtga tgacggcatt gttgcgcgca ttcctgacac
    10321 tgatggtaaa ttgcccgacg ccgcgatttt tttgtttgaa ccagaaaagt tgctgcaaat
    10381 tgtccgcgag gcggtaggca gttcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    10441 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    10501 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggccattct
    10561 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    10621 gcgtcgcctg aacggaggcg aaattcaaat atccgatgta acgactacta cgccctcgcc
    10681 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    10741 gctggcagaa cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    10801 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    10861 gttgcaacga ctggctcctg gcagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    10921 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    10981 agagattgcc tcgtatctgg aaaatcttct tacagtaaaa cgcatcttcc cgacgatgat
    11041 tagtggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgcg atgccctcgg
    11101 cgtacgatta ccagagtcac tgccagagat ttatttgcat agagtcagtt acccgcttcg
    11161 cgacctcttt ctacgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    11221 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gcgaacaggg
    11281 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    11341 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    11401 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    11461 tgcctcaaca tcgccaggcg tttatgaggg ggtggatggc gtgatgcggg tgatcgaaca
    11521 gcttgccgga gtcggcttac ccgcctcact ctgggaaagc cagatcttgc ctgcccgcgt
    11581 acacgactat tcgccagaag tgctcgatga attactggca accggtgcgg ttatctggtc
    11641 ggggcaaaaa aaactgggtg aagatgacgg cctggtggca ctgcatctac aggaatatgc
    11701 tgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcaacaagc
    11761 gatagtcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    11821 acgcgacaaa atcggcgaat cggttgatcc ctcggcactg caagaggcgt tatgggcgct
    11881 ggtctggcaa ggcgtcatca ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    11941 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    12001 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    12061 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    12121 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    12181 cgggtttcca tcgatgcaaa cgctttgtcg aagtatggaa gattccgggc gaattatgcg
    12241 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    12301 cttgcgcgac ctggcgacac aagccgcgca aacgcgccac tatacaccag tggcgctctc
    12361 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tggcctgcac atccggcaac
    12421 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    12481 ctatctggcg caaggtggca aaaaaatgct ggtctggcag gaaaaagagg aattactcgc
    12541 cccagaggtt ttccacgcgc tgactaccgc actgcgtcgc gaaccacggc tgcgctttac
    12601 gctaacagaa gtgaatgatc taccggtctg gcaaacgccg atgtttacgc tgctgcgtga
    12661 agcgggattt tcaagttcgc cacaagggct ggattgggga tagagaaagg actgacggat
    12721 gcccgttcgc atccgtcagt attgcaggac ggattattcc gcgtccggct cttcagactt
    12781 gtatttagcg gcagtttctt tgatcagctg ctgcagttcg ccacgctgat acatttcgat
    12841 cacgatatca caaccgccta ccagctcgcc gtcaacccac agttgcggga aggtcggcca
    12901 gttagcatat ttcggcagct ccgcacgaat gtccggattc tgcagaatat caacataggc
    12961 aaaacgttcg ccacatgcgg caagcgcctg gactgcctgg gcagagaaac cgcagctcgg
    13021 cagtttcggt gaacctttca tgtacagcag gatcgggttt tcagcaatct ggcgttggat
    13081 tttttcgata gtggtgctca ttgtcttgct tcctcaaact tctttacggc agtaatctga
    13141 cattgtagcg ggtcagtgcg gcatcggaaa ataacatttt catcacgctt ttgctatttt
    13201 atccctttgc tctatttgtt gcatttcaaa tattcgtttc ttccctgatg cacggcaaac
    13261 tggcgtattt atgaccattt ttagctttca ctgctgcatt tttttgcgct cgccaacgaa
    13321 acgtattttt taacaataaa agctattaac tttctcttct tctatgcatt agaatcatca
    13381 agttttgtaa atcagacgca ggcatgatag acctgcctta acagagggac gctcagtggc
    13441 gcggataaac cgtatttcga tcacgctctg tgctttactt tttaccaccc tgcctttaac
    13501 gcctatggcc catgcttcaa agcaagccag ggagagttct gctaccactc atatcaccaa
    13561 aaaagcagat aaaaagaaaa gcacggcaac caccaaaaag acccagaaaa cagcgaaaaa
    13621 agccgccagt aaaagtacga ccaaaagcaa aaccgcttct tccgttaaaa aatcttccat
    13681 taccgcttct aaaaacgcca aaactcgcag caaacatacc gtcaataaaa cggcctcagc
    13741 cagctttacc gaaaagtgta ccaaacgtaa gggctataaa tcgcattgtg tgaaagtcaa
    13801 aaatgcagcg tcaggaaccc ttgccgacgc acacaaagcg aaggtgcaaa aagccacaaa
    13861 agtggcaatg aataaactga tgcagcaaat tggtaagcca tatcgttggg gtggcagctc
    13921 accgcgtacc ggttttgatt gcagcggcct ggtttattac gcttataaag atttggtgaa
    13981 aattcgtatt ccgcgtacgg cgaatgaaat gtatcacctg cgcgatgcag cgccaatcga
    14041 acgtagtgaa ctgaaaaacg gcgacctggt ctttttccgt actcagggac gcggtacagc
    14101 cgatcatgtc ggcgtgtatg tcggcaacgg caaatttatt cagtcaccgc gcacaggtca
    14161 ggaaattcaa atcacttctc tcagtgaaga ctactggcag cgccactatg ttggcgctcg
    14221 ccgggtaatg accccaaaaa cacttcgcta aaactttacc ctgttgttac ggcaacaggg
    14281 taagttcatc ttttgtctca ccttttaatt tgctacccta tccatacgca caataaggct
    14341 attgtacgta tgcaaattaa taataaagga gagtagcaat gtcattcgaa ttacctgcac
    14401 taccatatgc taaagatgct ctggcaccgc acatttctgc ggaaactatc gagtatcact
    14461 acggcaagca tcatcagact tatgtcacta acctgaacaa cctgattaaa ggtaccgcgt
    14521 ttgaaggtaa atcactggaa gagattattc gcagctctga aggtggcgta ttcaacaacg
    14581 cagctcaggt ctggaaccat actttctact ggaactgcct ggcaccgaac gccggtggcg
    14641 aaccgactgg aaaagtcgct gaagctatcg ccgcatcttt tggcagcttt gccgatttca
    14701 aagctcagtt tactgatgca gcgatcaaaa actttggttc tggctggacc tggctggtga
    14761 aaaacagcga tggcaaactg gctatcgttt caacctctaa cgcgggtact ccgctgacca
    14821 ccgatgcgac tccgctgcta accgttgatg tctgggaaca cgcatattac atcgactatc
    14881 gcaatgcacg tcctggctat cttgagcact tctgggcgct ggtgaactgg gaattcgtag
    14941 cgaaaaatct cgctgcataa taactgatgg caaatgcagt attgcctgat gcgcttcgct
    15001 taccatgcca acgcggatga ggcgttaaca gcgcattcgg caagataaat cgcacgttgt
    15061 cagcaactgt aacgcagaag gttatccttc tgcgtttttg tttaattagc tgttagcaac
    15121 gcaaactgtt tcaggttgtt ttctggctga cataaacacc agcaataatg ccagtcccgc
    15181 gacaatcgcc cccatcaccg gcacaaagct gtatcccagc ccagcggaaa ttaccgcacc
    15241 accagcagct gctcccagcg catttccaag attaaaggca ccaatattga ctgatgaaga
    15301 cagacctggc gcttcactgg cgacacgcat cacgcgcatc tgtaacggcg gtacgaccgc
    15361 aaaggttgca gcgccccaca ccgccatgct aatagctgcg ccgaacttat tgcgggccag
    15421 gaacgggatt gccagcataa tcaccatcag cagcaacaaa aagcctttca acgtgccgtt
    15481 aactgaacga tctgccagtt tgccgccgag atagttaccg atagagaatc cgacaccaat
    15541 cagcaccagc attgccgtga cgaacaccgg tgttgcgtgg gtaatacttt gcagtaccgg
    15601 agagatatag gtgtagagag taaacattgc accagctccc agtaccgtcg tcagcaatgc
    15661 agacagcacc tgcggacgca ttaataccgc cagctctttt ttcacttcag gtcgtgcccc
    15721 tgcaccacct ttaggtaatg agaagaacag acttaccatt gaaatcactc ccagccccgc
    15781 cgttgccaga aatgacatcc gccagccgat ggtttcaccc aaccaggtcg ccgccggcac
    15841 gccaccgata tttgccaggg ttaaccccat aaacatagtg gcaactgcgc tggcctgttt
    15901 atgttttggc accacgcttg cggccacgac tgaacccaaa ccaaaaaatg ctccgtgatt
    15961 caggctggtc aaaatgcgtg aaagcatcag ggtcatataa tccggcgcga tggcggaaag
    16021 tacgttgccg agcgtgaaaa ttgccatcag gaaaatcaac gcactgcggc gggcacgatg
    16081 agaaagtaga agcgtcatca gcggcgcgcc aaccattacg ccaactgcat aggcactgat
    16141 taacattccg gcagcgggaa tcgagacatc cacaccgcgc gcaatgacgg gcaacaagcc
    16201 cattggcgag aactccgttg tcccgatacc aaacgcgcca atcgccagcg ccagcaacgg
    16261 atagttaatt ttcatgcctt atctccacct cttcgcgtca ttacgcgata ttcattaaag
    16321 tggcgaaagc atgacagcaa tcacaaaaaa atgaaaataa caaaaagaga aaacactttt
    16381 gccattttgc taacaaacag gaaggagatg cgagggagaa cgcgctccct cgagaggaaa
    16441 tcagtgcagc gcggcagtca aacccacggc cacgatcaaa ccgaggacga taatcgttgt
    16501 taccagtgaa aatttaaggt cggtgctcat caagttttct ccttttttat taccacacaa
    16561 aaagtgatat tacgcatttt tacacactgt gatgaaaaaa tctcccgtca tttataatga
    16621 taagtgtttt taccacttcc ccttttcgtc aagatcggcc aaaattccac gcttacacta
    16681 tttgcgtact ggccattgac cccttcctga cgctccgtgt cgtttttccg gcgtaccgca
    16741 acacttttgt tgtgcgtaag gtgtgtaaag gcaaacgttt accttgcgat tttgcaggag
    16801 ctgaagttag ggtctggagt gaaatggaat ggcaacaata aaagatgtag cgaaacgagc
    16861 aaacgtttcc actacaactg tgtcacacgt gatcaacaaa acacgtttcg tcgctgaaga
    16921 aacgcgcaac gccgtgtggg cagcgatcaa agaattacac tactccccta gcgcggtggc
    16981 gcgtagcctg aaggttaacc acaccaagtc tatcggtttg ctagcgacca gcagcgaagc
    17041 ggcctatttt gccgagatca ttgaagcagt tgaaaaaaat tgcttccaga aaggttacac
    17101 cctgattctg ggtaatgcgt ggaacaatct tgagaaacag cgggcttatc tgtcgatgat
    17161 ggcgcaaaaa cgcgtcgatg gtctgctggt gatgtgttct gagtacccag agccgttgct
    17221 ggcgatgctg gaagagtatc gccatatccc aatggtggtg atggactggg gtgaagcaaa
    17281 agctgacttc accgatgcgg tcattgataa cgcgttcgaa ggcggctaca tggccgggcg
    17341 ttatctgatt gaacgcggtc accgcgaaat cggcgttatc cccggcccgc tggaacgtaa
    17401 cactggcgca ggccgccttg ccggttttat gaaggcgatg gaagaagcaa tgatcaaggt
    17461 gccggaaagc tggattgtgc agggtgactt tgaacctgaa tccggttatc gcgccatgca
    17521 gcaaatcctg tcgcagccgc atcgccctac tgccgtcttc tgtggtggcg atatcatggc
    17581 aatgggcgca ctttgtgctg ctgatgaaat ggggctgcgc gtcccgcagg atgtttcgct
    17641 gatcggttat gataacgtgc gcaacgcccg ctattttacg ccggcgctga caacaatcca
    17701 tcagccaaaa gattcgttgg gtgaaacagc gttcaacatg ttgttggatc gtatagtcaa
    17761 caaacgtgaa gaaccgcagt ccattgaagt gcatccgcgc ttgattgaac gccgctccgt
    17821 ggctgacggc ccgttccgcg actatcgtcg ttaatcaccc gttgcgggag tctcttccgg
    17881 ctcccgcagc cactccttat tcagcgtctc actatcgccg agatactcaa gcaaccaggt
    17941 taacgcaggc gacatatcat tttgctgcca tgtcagacaa catgccgaat ccggaaaggg
    18001 gttttccagt tctaatgcta cccacttccc ctcattaagc cacggtttgg cgaaatgtgt
    18061 tggcaccatc cctatgcata atcctgccga gatacaggtt gccgatgatt cccagtcagg
    18121 cacgacaact cttttttggt tatccagcaa ccaggtaata cgcttaggta gcgttcgcga
    18181 ggtgtcttcg cgcaccaacg acggccagtt gcgcaacgta tcatcgctga acgggccatc
    18241 catcaacgcc agcgggtgat ggctggcaac aacgcaactc cagcttagca tccccatatc
    18301 ccggaaggca taacgaccgc ctaccggaat cgcgcgtgtt gcgccaatcg ccagttccac
    18361 gcgcccgtcg gaaagcgcat cccagacacc gttgaacact tcctgaaaga caagaagttc
    18421 gacatcatca aaatggcgat aaaaatcaac gatcatctgc cgtgtacgtt ctggcctgac
    18481 aatattatcc actgcgatag ctaactgacc gcgccagccg ttcgctatct gctgacattg
    18541 ctggcgggtg atctgcattt ttttgacaac agagcgccct tctttgagaa accacgctcc
    18601 ggcagcggtc agctccacat cacggtgccg tcgttcaaac agcggcaccg ccagccactc
    18661 ttccagctga cgcacggtat agctgaccgc agaaggaacg cgatgcagct cctgtgccgc
    18721 agcgctaaaa ctaccattac gcgctaccgc atcaacaact tcgagtgaat attctgacca
    18781 catagtctgc ctgcaaaatt tttgaaacca gtcatcaaat attaccgttt cacaacacta
    18841 atttcactcc ctacactttg cggcggtgtt taattgagag atttagagaa tatacatgca
    18901 acctgggaaa agatttttag tctggctggc gggtttgagc gtactcggtt ttctggcaac
    18961 cgatatgtat ctgcctgctt tcgccgccat acaggccgac ctgcaaacgc ctgcgtctgc
    19021 tgtcagtgcc agccttagtc tgttccttgc cggttttgcc gcagcccagc ttctgtgggg
    19081 gccgctctcc gatcgttatg gtcgtaaacc ggtattatta atcggcctga caatttttgc
    19141 gttaggtagt ctggggatgc tgtgggtaga aaacgccgct atgctgctgg tattgcgttt
    19201 tgtacaggct gtgggtgtct gcgccgcggc ggttatctgg caagcgttag tgacggatta
    19261 ttatccttca cagaaagtta accgaatttt tgcgaccatc atgccgctgg tgggtctatc
    19321 tccggccctg gctcctctgt taggaagctg gctgctggtc catttttcct ggcaggcgat
    19381 tttcgccacc ctttttgcca ttaccgtggt gctgattctg cctattttct ggctcaaacc
    19441 cacgacgaag gcccgtaaca atagtcagga tggtctgacc tttaccgacc tgctacgttc
    19501 taaaacctat cgcggcaatg tgctgatata cgcggcctgt tcagccagtt tttttgcatg
    19561 gctgaccggt tcaccgttca tccttagtga aatgggctac agcccggcag ttattggttt
    19621 aagttatgtc ccgcaaacta tcgcgtttct gattggtggt tatggctgtc gcgccgcgct
    19681 gcagaaatgg caaggcaagc agttattacc gtggttgctg gtgctgtttg ctgtcagcgt
    19741 cattgcgacc tgggctgcgg gcttcattag ccatgtgtcg ctggtcgaaa tcctgatccc
    19801 attctgtgtg atggcgattg ctaatggcgc gatctaccct attgttgtcg cccaggctct
    19861 gcgtcccttc ccacacgcaa ctggtcgcgc cgcagcgttg cagaacactc tgcaactggg
    19921 tctgtgcttc ctcgcaagtc tggtagtttc ctggctgatc agtatcagca cgccattgct
    19981 caccaccacc agcgtgatgt tatcaacagt agtgctggtc gcgctgggtt acatgatgca
    20041 acgttgtgaa gaagttggct gccagaatca tggcaatgcc gaagtcgctc atagcgaatc
    20101 acactgatct atatcgatat acttatactt aggctgctaa caaaattttg ttgtatgatt
    20161 gaaattagcg gcctatacta atttcgagtt gttaaagcta cgataaatat tatgttttta
    20221 cggggacagg atcgttcccg actcactatg gatggtcatt tcggcaaggg ttcctccttt
    20281 ccctctgttc tacgtcggat tatagactcg cggttttttc tgcgagattt ctcacaaagc
    20341 ccaaaaagcg tctacgctgt tttaaggttc tgatcaccga ccagtgatgg agaaactatg
    20401 agttcatcgt gtatagaaga agtcagtgta ccggatgaca actggtaccg tatcgccaac
    20461 gaattactta gccgtgccgg tatagccatt aacggttctg ccccggcgga tattcgtgtg
    20521 aaaaaccccg atttttttaa acgcgttctg caagaaggct ctttggggtt aggcgaaagt
    20581 tatatggatg gctggtggga atgtgaccga ctggatatgt tttttagcaa agtcttacgc
    20641 gcaggtctcg agaaccaact cccccatcat ttcaaagaca cgctgcgtat tgccggcgcc
    20701 cgtctcttca atctgcagag taaaaaacgt gcctggatag tcggcaaaga gcattacgat
    20761 ttgggtaatg acttgttcag ccgcatgctt gatcccttca tgcaatattc ctgcgcttac
    20821 tggaaagatg ccgataatct ggaatctgcc cagcaggcga agctcaaaat gatttgtgaa
    20881 aaattgcagt taaaacctgg gatgcgcgta ctggatattg gctgcggctg gggcggactg
    20941 gcacactaca tggcatctaa ttatgacgta agcgtggtgg gcgtcaccat ttctgccgaa
    21001 cagcaaaaaa tggctcagga acgctgtgaa ggcctggatg tcaccatttt gctgcaagat
    21061 tatcgtgacc tgaacgacca gtttgatcgt attgtttctg tggggatgtt cgagcacgtc
    21121 ggaccgaaaa attacgatac ctattttacg gtggtggatc gtaatttgaa accggaaggc
    21181 atattcctgc tccatactat cggttcgaaa aaaaccgatc tgaatgttga tccctggatt
    21241 aataaatata ttttcccgaa cggttgcctg ccctctgtac gccagattgc tcagtccagc
    21301 gaaccccact ttgtgatgga agactggcat aacttcggtg ctgattacga tactacgttg
    21361 atggcgtggt atgaacgatt cgtcgccgca tggccagaaa ttgcggataa ctacagtgaa
    21421 cgctttaaac ggatgtttac ctattatctg aatgcctgcg caggtgcttt ccgcgcccgt
    21481 gatattcagc tctggcaggt ggtgttctca cgcggtgttg aaaacggcct tcgagtcgct
    21541 cgctaaaggc tattctatcg ccccctctcc gggggcgatt tcagatcagg cttctgtacc
    21601 tggttgattc atggcatttt ctcgtgccgc cagcacacgt tctaccgtat ctaccactgc
    21661 ctgagtttgt ggatcgattt caatgttgac gcgtgcgcca agttttttct tcccaagagt
    21721 cgtgcgttcc agagtttccg gaattaaatg gacgcaaaaa cgcgttggcg tgacttcgcc
    21781 gacggtcagg ctaataccgt cgatgccaat aaatcctttg tacaggatat atttcatcaa
    21841 ctgactatcc tggactttaa accagatctg gcgattattt tccgaggtta atattttcgc
    21901 cacttcagcg gtggtcataa tatgacctga cattaagtgt ccgccaattt catcactgaa
    21961 tttcgccgca cgctcaacgt ttacccaatc ccccactttt aaatcgccaa gattggtaat
    22021 gcgcaacgtt tctttcatca ggtcaaaact gacatggttg ccgttaattt ccgtcacggt
    22081 caggcagcaa ccgttatgcg ccacggaggc accggtttcc agaccgtcca gcatgtggtc
    22141 gggtaactcc accacatgtg tgcgaaaatt tggtttctcg tcaatcgaca ccagttttgc
    22201 ggtgccctgt acaatccccg taaacatact tacaactcct gaaatcagtt aagacattct
    22261 gttcagcaca atagcaggtg gaaaacgccc ttaccagtga aggggtaaga atggctattt
    22321 tttcactgga gaattaataa atcctcgcta caatagactg aatttcccct gcttcttctt
    22381 tttgctgcct attcaggcgg ctttttagtc tctcatataa ctacaaataa aaggtgttca
    22441 cgtgcagaag tatatcagtg aagcgcgtct gttattagca ttagcaatcc cggtgattct
    22501 cgcgcaaatc gcccaaactg cgatgggttt tgtcgatacc gtgatggcgg gcggctatag
    22561 tgccaccgac atggcggcgg tcgctatcgg tacttctatc tggcttccgg cgatcctctt
    22621 tggtcacgga ctgctgctgg cattaacgcc ggttatcgcg caattaaatg gttccggtcg
    22681 acgtgagcgc attgcgcatc aggtgcgaca gggcttctgg ctggcgggtt ttgtttccgt
    22741 tctcattatg ctggtgctgt ggaatgcagg ttacattatc cgctccatgg aaaacatcga
    22801 tccggctctg gcggacaaag ccgtgggtta tctgcgtgcg ttgctgtggg gcgcgccggg
    22861 ctatcttttc ttccaggttg cccgtaacca atgtgaaggt ctggcaaaaa ccaagccggg
    22921 tatggtaatg ggctttatcg gcctgctggt gaacattccg gtgaactata tctttattta
    22981 tggtcatttc ggtatgcctg agctcggtgg cgttggttgt ggcgtggcta ctgcggcggt
    23041 gtattgggtc atgttccttg ccatggtttc ttacattaaa cgcgcccgct cgatgcgcga
    23101 tattcgtaac gaaaaaggca ccgcgaaacc cgatcctgcg gttatgaaac gactgattca
    23161 actcggtttg ccgattgcgc tggcactatt ctttgaagtg acactgtttg ccgtcgtggc
    23221 tctgttagtg tctccgctcg gtattgttga tgtcgcagga caccagattg ccctgaactt
    23281 tagttcacta atgttcgtgc ttccaatgtc gctggcggca gcggtaacta tccgcgtagg
    23341 ttatcgtctg ggtcagggct caacgctgga tgcgcaaacc gctgcgcgga ccgggcttat
    23401 ggtgggtgtc tgtatggcaa ccctgacggc catattcacg gtttcactgc gggagcaaat
    23461 cgccctgttg tacaacgaca atcccgaggt tgtaacgctg gcggcgcatt tgatgttgct
    23521 ggcggcggta tatcagattt ctgactcaat ccaggtgatt ggcagtggga ttttgcgtgg
    23581 ttataaagat acgcgttcca ttttctatat cacctttacg gcttactggg tgctgggctt
    23641 gccaagcggc tatattctgg cactgaccga tctggtcgtt gaacctatgg ggccagcagg
    23701 cttctggata ggctttatta ttggcctgac gtcggcagcc attatgatga tgctgcgtat
    23761 gcggtacctg caacgtctgc cgtcagccat cattctgcaa cgagcatccc gctaataaag
    23821 acaaggcgca accttcacgg ggtgcgcctg tgtttttacg caggctggag cgttgcgcca
    23881 atcccgtctt cgtctggctg tagtttcagt gcgttacaca gagtttgccg actggcatct
    23941 gttatctgaa cgcgtatatc cccgttaact tcccctttca cattgatgaa gtcaggtgaa
    24001 atttctttcg cactggtttg caggcaaatc gctccctctc cgcgcaggcg tttcactttc
    24061 agctgcacat gcgttgccgc accagtgatc agccacagtt ttccgccatt cagattgatg
    24121 ttaccaatac tgaaagtgac actgtcacct tttaccgtac tgccgtcgag aatcaataca
    24181 ccgccctgct gtacagtaat cgttgattgt tgcagatctg cgccatagcc aataatcatt
    24241 gaagcgccgt ccagtaatgc aacactcccg gtcagttcaa ccggtgcgcg atcttcctct
    24301 acgacaaaat tttcgtccgc gctggtgggt gacaacagga tcactgttcc accttgtatt
    24361 accgcatttt ccagcgtacc ggcatgcact tcagcccgac cacccacatt aacggacagg
    24421 ttttgcgtct taccggaact gtagagctga agaccatccg tccccagacg atagatggct
    24481 ccattctcaa caatagtgtc agagtcttca ccaccgagat tattcatggt gccatcagca
    24541 agcaacgtat cattggcttt ccccgccagc ataaaaacgc cgccctgatt aatctcacaa
    24601 ttgctggctt ccccacctgg atacaccaac aattcaccac cttcatctac cacgaccgct
    24661 gaggttgtcc cattaaccaa caggccgccc tcttgatcga gaatgatttt ttccgcgcga
    24721 tgtccttcca gtacacgcag gttaccgcca ttttccagca acaaaccgca ggcataaccc
    24781 tgatcaacgc tgaattcacc ttcgggatgg cggccattaa ccgtagcgag cgtagagaga
    24841 ctaattgcgc ctccctcgga ctgaacaata tctgtggcaa tggcaccgtc gctgaccgat
    24901 agcgtgccat tgttaccgta ctgtgtgcat ttggcgatcc caccggttgc cactttcaac
    24961 cagccagccc ccgtgaccac ggtattttcg gctcttccgc cagcttcaac aatcaactcg
    25021 ccttcagtaa gttgcgtcgc gccaatgacc tcgccggtct taacaatttg cacattcccg
    25081 tcgctcatca agtttttcgc atcagatccc atgatttatt cctttgctgc atctgtgtgc
    25141 ctttattgct acctaagtgt aaaggctacg gaggatttat ccacgacaga tttgagatgg
    25201 tggcaaacaa ctctgtttaa actctgatac acgaattatt gggttgtatc agatgtaaat
    25261 gcgatcctga ataaaaatcg cccttgcaaa tcaacaaaat atagccaatt tgcttaaaag
    25321 ctctgcaaac ggtcgaaata gcgcagaaaa ttacgttttg cctcttgcca ccttcccact
    25381 ctgccgctaa tattcgtccc cgttgtcacc tacaacgttg cgttcatagc tcagttggtt
    25441 agagcaccac cttgacatgg tgggggtcgt tggttcgagt ccaattgaac gcaccatcct
    25501 gcgtccgtag ctcagttggt tagagcacca ccttgacatg gtgggggtcg gtggttcgag
    25561 tccactcgga cgcaccagat tttcttaatc tggtcttctc ctttttccct ctgcttcttc
    25621 tctgtatcca atacgttaaa agatttacac tgtcttcgta tgcgttatca gaaggagaat
    25681 cgctatggca actttgttac aacttcattt tgcttttaat ggcccatttg gcgacgcaat
    25741 ggctgagcag cttaaaccac ttgcagagtc gattaatcag gaacctggtt ttctgtggaa
    25801 ggtatggaca gaaagtgaaa agaaccacga agccggtggg atctaccttt tcactgatga
    25861 aaaaagcgcc cttgcctatc tggaaaaaca cactgcccaa ctgaaaaaac tcggcgttga
    25921 ggaagttgtc gccaaagttt tcgatgtcaa tgagccactg agtcaaatca atcaggcaaa
    25981 actcgcctga cagaatttaa tcaagggcgg ttagcgccct tttcatccct gtctgaaatt
    26041 tctcaaattc taaaaatctc aaccaaactt atctgataac actaaattcg aaagaatgcg
    26101 tacaggtaag taacaatgaa aaaaattgca attgtgggtg ccgggcctac ggggatctac
    26161 accttattct cgcttctaca gcaacaaact ccactttcta tttctatctt cgagcaggct
    26221 gacgaggccg gtgtcgggat gccatacagt gatgaggaaa actcaaaaat gatgctggca
    26281 aatattgcca gtattgaaat accgccgatt tattgtacgt atctcgaatg gctacaaaag
    26341 caagaagcca gtcatctcca gcgttatggc gttaaaaaag aaaccttgca cgatcgtcag
    26401 tttttaccgc gaattctgct gggcgaatat ttccgcgatc aatttttacg attagtagac
    26461 caggcacgaa agcaaaaatt tgcagtggct gtttatgaat catgccaggt taccgatctg
    26521 caaattacaa atgctggcgt catgctcgct acaaatcagg atttacccag cgagacgttt
    26581 gatttagcgg tgatcgccac gggtcacgtc tggcctgatg aagaagaagc aacccgaacg
    26641 tattttccaa gcccgtggtc aggcttgatg gaagcaaagg tcgatgcgtg taacgtgggt
    26701 attatgggaa catccttgag cggactggat gcggcaatgg cagtggctat tcagcatggt
    26761 tcgttcattg aagatgataa acaacacgtc gtttttcacc gcgataacgc aagtgaaaag
    26821 ctaaatatta cgttaatgtc gcgcacgggt attttacccg aagccgattt ctattgccct
    26881 attccctacg agcccttaca catcgtcact gatcaggcat taaatgctga gattcaaaaa
    26941 ggcgaatatg gccttttgga tcgggtattt agattgatag tagaggaaat caagtttgct
    27001 gatccagact ggagtcaacg catagcctta gagagcctga atgtcgattc ctttgctcaa
    27061 gcctggtttg ccgagcgcaa acaacgcgac caatttgact gggcagaaaa aaatctccag
    27121 gaagtcgaac gcaataaacg agaaaaacat actgttccct ggcgttatgt cattctgcgc
    27181 ctgcatgaag ccgtacagga aattgttcca catctgaatg aacacgacca taaacggttc
    27241 agtaaaggcc ttgcccgggt tttcatcgat aattatgcgg caatcccttc agagtctatt
    27301 cgtcgcctac ttgccttacg tgaagcggga atcattcata ttctcgctct cggtgaagac
    27361 tacaaaatgg aaattaacga gtcgcgcacc gtcctgaaaa cggaagacaa cagctactcg
    27421 tttgacgttt ttattgatgc ccgcgggcag cgtccgctta aagtgaaaga tattcctttc
    27481 cctggactac gcgaacaatt acagaaaaca ggggatgaaa tccctgatgt tggtgaagat
    27541 tatacgttac agcaacccga agatattcgt gggcgcgtag cgttcggcgc gttgccctgg
    27601 ttgatgcacg accagccttt cgttcaggga cttacggcat gtgcagaaat tggtgaggcg
    27661 atggctcggg cggtcgtaaa gcctgcatcc cgtgctcgtc ggcgtctttc gtttgattaa
    27721 aggtaaagct atcagcactc aacaaactga tgctgttcat taagacgata gcggactccg
    27781 gctcgaatat tgttttcacc ttcaatggcg acggcgaagc gaactctttc gccgtcatga
    27841 tatgccagtg cggcgctgcc ccctggcccg agaataattg agtcgaccac gcccgtgctc
    27901 actaccgttg aattttcacc gctggcaatg atgtgtacat tgtcgccgct gttggcaatt
    27961 ctggcgtttg cgccaaagct ggcgatttgc accaggtcac cattactggc gacatgacac
    28021 cgtttaccca atgtacaaac gcgtactcgc attccggtat tcgcaatccg actgctatct
    28081 ccagcgctgc ttatccgcga agaattgccg gttacagcaa cccgtgcgcg ctcaccggag
    28141 ctgccaatgt gactgttaaa gccgacactt ccaatccgca cgctataacc catactggca
    28201 atttgtgcag catacccagc ggttgcgatt ctggcgttat cctcacaaca gccgatgcga
    28261 gcatgatcgt cgctgcgggc aaactgactg caaaatgctc tctctccggg gtctgtggcc
    28321 agtttttgca tagaggaaac ttcctgatgc gcgaagtttt catccgccag ccattttttc
    28381 cacgcatact caaccagact ctccgcccag tccgtatatc cttcctcaat cagtgcatga
    28441 tgaatatcgg cataactacc gccgcgtggg aaatggcgca ggaaccagcg atacatcacc
    28501 gctccgatac gccattctcg caggtctgct cgtgtaatga tcattcgtta gtgccggtga
    28561 tagccatcga ccatgctttt aaaggtttcg tgtggcgtac gccccgtggt gcaaagataa
    28621 agatgaccga agataaaaaa gaggcttata aatgccagag caaaatgcgc ctgcaataac
    28681 cagtatctta cgccaggaaa cacatctccc acggcttgcg gatagagaca cagcagcccc
    28741 gtcaatagta acaacggcag caatccatac atgacaccaa cataggcaac ctgctgtaag
    28801 ggattaaatt tagactgggt tgttgccggg aaaggatgtt cttccccctg cataatgcca
    28861 aacaaataaa atcgcgtttg ttttgccgcc cgctccagcc aaccctgacg acgaatgcga
    28921 tagtggtggc cattaccccc aacgccattg atcagaacaa agccgagcca gcatgccagt
    28981 aacaaaaatc cgcaaacttc atgcaccgca accagacttt taaccgcagt tgcgcccacc
    29041 agcgcaaagt gatttatcag cccacttgcc agcaacaata caaagagtaa cgcattcgac
    29101 cagtgccata atctgaccgc tttgctgtaa agataaatcc tctcaccatg atcggtggct
    29161 gatttcttca cgccacgagc acgaagcaag gcgtgcagcc ccagcaccaa ccacatccca
    29221 accagcaata ctccggcaat gatcaaccac accggccaaa attcgggagt gaactgtggc
    29281 acatagttcg ctaactggct ctgaaactgt tcagcatgtt gcgacgggtt catacatttt
    29341 cctttttaat caaatgttga ccgaaccggc gatacagatg cggtttcccg gcaccaggta
    29401 gctgatattg atagtattta ttatcctgca accacgcctg aatttcaggg ctatcttcgc
    29461 gaccaaagat caatgcgtgt tccgggcaag cgctgacgca aatgggcgga aagccttttg
    29521 ccagtcggga ctcagcgcag aaatcacatt tatctgccac tttagtgaca gggttaaggt
    29581 agcgaacctg gtacggacaa gcgccaatgc aataactgca gccgatgcac tgcgattttt
    29641 ctacccgcac gatcccctgc tcatcacgcc acgacgcccc tgtcgggcat acgtcgatgc
    29701 acggagcatc ttcgcagtgc tgacatgact ggcgaaaaaa gtgatactgc gtttcgttgt
    29761 cgttatcggt gactggaatg tgtgctatcg ataaacggct tccctgagcg ggggcgtggt
    29821 tagttttacg gcatgcgcgg gcgcaaatat tacagccatt gcaccgtgac tcgtcatgaa
    29881 tcatggcata ccgaacttcc ttttcttgcc tcgtgttcgc taacagagaa gacgcagaac
    29941 cagtaaaaaa gattactgtt cccatcccca gaacaaattt gcgtcgagtg aaggacatct
    30001 gattatcccc taatgcagtg gctgcaactg tccaccctat ccatgacatc tccctgaaat
    30061 ttcaggggtt ctgaaactgc tctacctcga accattcgca taagccataa gccttgccat
    30121 aggtcataaa tcgctcgcta aacaagtaca acaccgccgt cgatgactct acctgccgta
    30181 tatcggcata ttcaggtgca acatccattg cggcgatcgc ggcttcaatc tgcgcttcct
    30241 ggaagtaata tggcgcttgc atcagcatgg cgactttgta aggacgagga tacgtctggc
    30301 actcaaaacg aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca acttgcaaag
    30361 acatcgccgc gtagttttca ctcatggcct gcgttgagta atagtaatca tcctgacttc
    30421 ccgtcataca agcgatatcc gcgtagtctc cgttctgctt gatgttttcc agcaaaactg
    30481 acagttcctc ttctgcaact gaatagggtg gcgagagaaa cacggcgcgc gccaccagct
    30541 gcccagccac agaatgctgg cgaatgaact ctgccaggat ttcagccggt tctggttcgg
    30601 ttaccgtatc aacggggtga cgcatcccct gcaaccattg ccgcttcgcc tcgtcaactt
    30661 cgctgacttt cgctaacggc aattcatcct gatggttcat cttacccatt ccttacgcag
    30721 gcagtagatt gtgtgctgcc agatcggcgg caatatcttc cagccccaga cgctgcaatg
    30781 tttcgcgggt cggacaacca agctgtgggt cccagcccat ttctttgtag aacatcgtga
    30841 gcgaggcatg catatcatca cgatccattt tgtcagtacc ttcagtaaag accgggatct
    30901 gcggatcctt gtcgaatacc caggaacaga taagatcgtg ttcattacgc atatctttgg
    30961 tttgcatcag tttcaccgtg taggcacgat gcaatgtaaa gatacgctct gcagctaaat
    31021 ctaatttttc ctgagtcatc tcttcgccag tgatcgcttt gaagaatttg gcttcaagcg
    31081 ccagatcgcc ccggtaatta cggcttttca gcggcgaaac ggtcattggc cagacccagt
    31141 tgcacagggt gacggcgtta tgcaaacaga cccgcaacag cgaccactta gcatatttaa
    31201 tttttgcgtc attgattggc gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa
    31261 aaagttcttt cgccacttca cgttgcagtt tcaatggcaa gccggaacca ataaagttga
    31321 tatgggtatg cgtcatgcag tcacggttga acatacagtt aacaatggaa ccgacctgcg
    31381 ctgacgcttc attggcatgg tgaaccggat agccaaacgg cgaccagagt ttatttttcg
    31441 cgtagcccca gtactcttca cccaaattcc agcattctgc gatggcatat gaaccatcag
    31501 ccaggtgact cagctcaccc acacgatgcg ccagacggta gtaaaaatct ttaatgaagt
    31561 taacgtctcc cgcttccagt tgatcccagc gaatttctgc atactcttca gcaggcagaa
    31621 cacgcttgaa cacacctttg ctgtaacaat aagtaaagtc gcgatgcaac tgcccgtagt
    31681 tacaccatag gccgtagtca tcgaacaaat tcagaccaac caggttacca atcacacgac
    31741 catcgtcttt atcttcaaaa tctttcggcc cgttcgggaa gatggtggta tggacaaagt
    31801 ttgctacaca agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat
    31861 tcatttgggt catacaacgg atcgggcaag agtggcagcc gctcattttt actgtgtatt
    31921 tctctgctgc cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtattct
    31981 gattgcctgg cggaatttca cccgtttcaa ttggaccgcc ttcagccgcg ccccaaaaca
    32041 gccctttacg tgctgtccag cgtgacttgg gatctgaata ctctgcccac gattgcggcg
    32101 tacttggcac gacatggttg ttattcgcac cgataagttc agtcatcatg taatcattga
    32161 gacgcttcat ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg
    32221 ctttcaggtt tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc
    32281 tattcaacat gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac
    32341 aggtttccgg actggtcaag cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc
    32401 ataagaaatc tgctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg
    32461 atttcgcctt cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa
    32521 aaaagccacc catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg
    32581 tgatatttac gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga
    32641 ctaatttatt cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt
    32701 tgtagccgaa gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa
    32761 tatttcctgt cgtgagattg actcttaata tattacctgt ccaaccgtta gtcatgattt
    32821 tttcctttgc aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt
    32881 tgggcaggcg tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt
    32941 atttacggtg gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacaacg
    33001 tttatgatcg acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca
    33061 gacgttcatg cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc
    33121 gccatacagg ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat
    33181 tttgatacgg gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc
    33241 acagcggtgg caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt
    33301 tatcaaccct actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa
    33361 cgcaggggca attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg
    33421 cgttaagcca atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc
    33481 gcaaacacag tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg
    33541 actctgtccc caacaccaga tattttccct aacccgatag tgtatgagaa aatatttaac
    33601 gccgataaac taatccttta tggttaggag aaaaaataat gtggttat
//
LOCUS       DAZKFZ010000030        32478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000030 DAZKFZ010000000
VERSION     DAZKFZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(243..1184)
                     /gene="fabY"
                     /locus_tag="ACHOQW_001343"
                     /gene_synonym="yiiD"
     CDS             complement(243..1184)
                     /gene="fabY"
                     /locus_tag="ACHOQW_001343"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP9572440.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1229..1666)
                     /gene="dtd"
                     /locus_tag="ACHOQW_001344"
     CDS             complement(1229..1666)
                     /gene="dtd"
                     /locus_tag="ACHOQW_001344"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9572441.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1663..2535)
                     /gene="yihY"
                     /locus_tag="ACHOQW_001345"
     CDS             complement(1663..2535)
                     /gene="yihY"
                     /locus_tag="ACHOQW_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP9572442.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2529..3128)
                     /gene="yihX"
                     /locus_tag="ACHOQW_001346"
     CDS             complement(2529..3128)
                     /gene="yihX"
                     /locus_tag="ACHOQW_001346"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP9572443.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3227..4012)
                     /gene="csqR"
                     /locus_tag="ACHOQW_001347"
     CDS             complement(3227..4012)
                     /gene="csqR"
                     /locus_tag="ACHOQW_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9572444.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4046..4942)
                     /gene="yihV"
                     /locus_tag="ACHOQW_001348"
     CDS             complement(4046..4942)
                     /gene="yihV"
                     /locus_tag="ACHOQW_001348"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFP9572445.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5110..6006
                     /gene="yihU"
                     /locus_tag="ACHOQW_001349"
     CDS             5110..6006
                     /gene="yihU"
                     /locus_tag="ACHOQW_001349"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP9572446.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6030..6908
                     /gene="yihT"
                     /locus_tag="ACHOQW_001350"
     CDS             6030..6908
                     /gene="yihT"
                     /locus_tag="ACHOQW_001350"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFP9572447.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKILSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6924..8165
                     /gene="yihS"
                     /locus_tag="ACHOQW_001351"
     CDS             6924..8165
                     /gene="yihS"
                     /locus_tag="ACHOQW_001351"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFP9572448.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8232..9134
                     /gene="yihR"
                     /locus_tag="ACHOQW_001352"
     CDS             8232..9134
                     /gene="yihR"
                     /locus_tag="ACHOQW_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFP9572449.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            9195..10070
                     /locus_tag="ACHOQW_001353"
     CDS             9195..10070
                     /locus_tag="ACHOQW_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9572450.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            10178..12214
                     /gene="yihQ"
                     /locus_tag="ACHOQW_001354"
     CDS             10178..12214
                     /gene="yihQ"
                     /locus_tag="ACHOQW_001354"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFP9572451.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            12260..13645
                     /gene="yihP"
                     /locus_tag="ACHOQW_001355"
     CDS             12260..13645
                     /gene="yihP"
                     /locus_tag="ACHOQW_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572452.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            13688..15091
                     /gene="yihO"
                     /locus_tag="ACHOQW_001356"
     CDS             13688..15091
                     /gene="yihO"
                     /locus_tag="ACHOQW_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572453.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            15158..15850
                     /gene="ompL"
                     /locus_tag="ACHOQW_001357"
     CDS             15158..15850
                     /gene="ompL"
                     /locus_tag="ACHOQW_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFP9572454.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15941..17206)
                     /gene="yihN"
                     /locus_tag="ACHOQW_001358"
     CDS             complement(15941..17206)
                     /gene="yihN"
                     /locus_tag="ACHOQW_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572455.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17308..18288)
                     /gene="yihM"
                     /locus_tag="ACHOQW_001359"
     CDS             complement(17308..18288)
                     /gene="yihM"
                     /locus_tag="ACHOQW_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9572456.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(18296..19006)
                     /gene="yihL"
                     /locus_tag="ACHOQW_001360"
     CDS             complement(18296..19006)
                     /gene="yihL"
                     /locus_tag="ACHOQW_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9572457.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19223..21046)
                     /gene="typA"
                     /locus_tag="ACHOQW_001361"
     CDS             complement(19223..21046)
                     /gene="typA"
                     /locus_tag="ACHOQW_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP9572458.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21419..22828
                     /gene="glnA"
                     /locus_tag="ACHOQW_001362"
     CDS             21419..22828
                     /gene="glnA"
                     /locus_tag="ACHOQW_001362"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP9572459.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            23002..24051
                     /gene="glnL"
                     /locus_tag="ACHOQW_001363"
     CDS             23002..24051
                     /gene="glnL"
                     /locus_tag="ACHOQW_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP9572460.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            24063..25472
                     /gene="glnG"
                     /locus_tag="ACHOQW_001364"
     CDS             24063..25472
                     /gene="glnG"
                     /locus_tag="ACHOQW_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP9572461.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25584..25694
                     /gene="yshB"
                     /locus_tag="ACHOQW_001365"
     CDS             25584..25694
                     /gene="yshB"
                     /locus_tag="ACHOQW_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP9572462.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25884..27257)
                     /gene="hemN"
                     /locus_tag="ACHOQW_001366"
     CDS             complement(25884..27257)
                     /gene="hemN"
                     /locus_tag="ACHOQW_001366"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP9572463.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27446..27952)
                     /gene="yihI"
                     /locus_tag="ACHOQW_001367"
     CDS             complement(27446..27952)
                     /gene="yihI"
                     /locus_tag="ACHOQW_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP9572464.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(28071..28237)
                     /locus_tag="ACHOQW_001368"
                     /pseudo
     CDS             complement(28071..28237)
                     /locus_tag="ACHOQW_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28534..29166
                     /gene="yihA"
                     /locus_tag="ACHOQW_001369"
     CDS             28534..29166
                     /gene="yihA"
                     /locus_tag="ACHOQW_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9572465.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(29548..32334)
                     /gene="polA"
                     /locus_tag="ACHOQW_001370"
     CDS             complement(29548..32334)
                     /gene="polA"
                     /locus_tag="ACHOQW_001370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9572466.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7867 a         8391 c         8034 g         8186 t
ORIGIN      
        1 ggttttgact atcaaaaatc gctcaaacct gaactaagat gatgaattaa gcattatctg
       61 cgataattgc tatacagctc tacaaataat atttaaatgc tcctgaacag tgatgtagca
      121 agacacgtat ggagccaacg aagaataaaa atttatagag ttcttattca atataaccga
      181 aatagccaag gactaatata aatgacctga atgagagagg cagcaccgcc cctctccatc
      241 agctactctt cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg
      301 atatacgtgc cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc
      361 tgcacccgtg cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg
      421 tcggctaccg catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc
      481 agaataatcg ttccgccgag gtggcgttcg cgcagcatca gccagataag cccccaaccg
      541 gtgagcgtcg ccagtgagaa taaactcccg gcaaacagcg tatggtgcgg attctgattg
      601 ccggtttctg gcatggtagt gataaatttt tgcccggtat attgctgaat gcgcacgccc
      661 attttttcac taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg
      721 ccgcgatgca aaatatcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc
      781 ggcgtggtgg ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac
      841 tccaccgcgt cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc
      901 gactccaggg tcatcgccat cagcgtgcct aaccctttgt cctgcacgtc gggatgaacg
      961 gccataaagc gaatggacgc ttcattgtcg gcattaatat acagtcggcc taccgccacc
     1021 agattaccct gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt
     1081 tccgaacctt ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa
     1141 cgctctaatt cttcttctgt ttgtggaacc cgaaggtgat acatagcgat actctctctt
     1201 gtttcccgtg acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat
     1261 cattgaccag cgatacctgc atatccgcag cgaagcgtcc tgtttgcgtg ttcatctctt
     1321 gctgacggca gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcac
     1381 ctttggagaa acttgggcgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa
     1441 ccaccagcac actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc
     1501 taaagatgcg gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat
     1561 ccttttcgac acccaataac accaaaagtc ccgcaccaat ttcgcccgtc acttctccct
     1621 ccacggtgac gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc
     1681 ttcttgttca gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc
     1741 gccaagcaag acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc
     1801 cagcacaccg taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccttt
     1861 ctttcctgct tcgaacagga gtgcagcaac aaacgcgccg acaatcgcgt cgcggttagg
     1921 tacgcggatg gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcaa
     1981 cggaaaaata cgcagcacgt tgtcgatgac agtattgaga tcgctcgccc agcgcaggga
     2041 gagcaaatag gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat
     2101 catccagtac acggcaaacg agtaaatttt gggtcgcgct cgtttactgc gccagatggt
     2161 attcaacgcg ctatcgatgg agtacatcaa caataacgcg gtgacgatca gcccgcacgc
     2221 cccaacggcg gtcatcttgt tggaattggc aacaaattgt tcgatatacc gctgaataac
     2281 atcgccagta gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc
     2341 ggaaaacatg ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga
     2401 gagcaacgac acataggcaa ggttacctgc cagggttgtc atattgtcct catcaatgcg
     2461 ttgccagagt agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg
     2521 aatggttttt agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc
     2581 agaatactgg taatgcccag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa
     2641 aagaccgtat cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgagct
     2701 tcaggtttgc gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt
     2761 tccgggtatt cttccggcca gaaggtggta tgcaggcggt tagtattgga aagcaccacc
     2821 acgcgatgcc cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca
     2881 acaaacaccg cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca
     2941 tgacacagcg cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac
     3001 gcctccccca tatgaaaact cttcttaagc gtagccagcg gaatacgcgt taaatcgctc
     3061 caggttccca gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata
     3121 tagagcattt ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt
     3181 ttggctatgt ggcaaggtat gaagccaggt caacagtcac aaaaatctac gcgtcttcct
     3241 ggggaaggat gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca
     3301 gctcgtcggt aaagagcgcg gtgacctgtg ccacgttacc aatttcaacc gccgccgaag
     3361 catgatactt agtgtgatcg gcaaccagca ggatatttct cgcgtgcgcc atcatcgttt
     3421 tcaccacgtt agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcaatcgccc
     3481 caacgcttgt taccagataa tcagcacgaa aatcggccac aaaggacgcc gctgaagggc
     3541 caatgatccc gctattatga gagcgcaacg taccgccggg taccatcact tcaaagcgcg
     3601 ggttgtggta aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat
     3661 ggttaagtaa cgcccgggca acatgctcaa cggtcgtacc aatggtgata aatattgttg
     3721 aaccatcagg aatatagtct gccaccgctt cggcaatcgc ttttttttcc tcggtttgcg
     3781 aaacctcacg ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt
     3841 gatggcgcgt aatcaggcct tgctcgctta atttacgaat atcccggcgc accgtctgcg
     3901 tggagacatc cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca
     3961 gcatgaggag ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc
     4021 ctcgaaaaac catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat
     4081 cacagtcagg gatcccggct cgtccaccgg gacgcgtgca tttcgacgcc gctacaccgc
     4141 tggcgaagcg gactgactcc gctaaatccc cacctgttgc cagcaccacc gccaatgcgc
     4201 cgtgaaaaac atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat
     4261 gttgacgccc gccattttcc agccagtcac agcctgcgct gccctgggtc acatagacat
     4321 gaccatttgt gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta
     4381 agcgcgccag acccggttct gaaaaggccg catgatcgct taatgccacc agctcgctga
     4441 tatcctgcgg cgtaatatca ccgtccagaa cggtcatcac gcccgcctgc cgggccagag
     4501 tgaaggcttt tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag
     4561 agaaatcaat ttcctccaac cactctgcgt caggcagcag gtccgggctg gggtagttaa
     4621 tgattatccg ctcgcctttg gcatccacca tgatggcgga ttgcgacgat ttcgcctgtt
     4681 tataccgttt ggtgtagcgg gtgttaacgc cccaggattc cagctctgcc agcaggctgt
     4741 tgccggtgtc gtcatcaccg acgcgcccaa taaaatcaac ctgcgctccc agtcttgccg
     4801 ccgcaaccgc tgccgtcgcc gctggcccgc caccaacttc cgtataattt ctcgccacgt
     4861 atttaccgct ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac
     4921 ctacacaagc aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttaa
     4981 tttcaccaat tgtttaaaaa gtgacggctg tcaatttttt gactataaat tacaaatacg
     5041 atcaaaaaca gacaatcaaa gcgtgtcata aatgacaaaa agtgacatca ctgtattcag
     5101 gagaaggtta tggcagcaat cgcgtttatc ggtttaggac aaatgggttc gccaatggcg
     5161 agcaatttat tgcagcaagg gcaccaactt cgcgtctttg atgtgaatgc cgaggctgtg
     5221 cggcatctgg tagacaaagg cgcgactccc gccgccaacc cggcgcaggc cgctaaagat
     5281 gccgaattta tcattaccat gttgccgaat ggcgatctgg tgcgcagcgt gttgttcggt
     5341 gaaaacggcg tttgcgaaag cttatctacc gatgcgctgg tcattgatat gtcgaccatc
     5401 catccgctgc aaaccgataa attgattgcc gatatgcaag ccaaaggctt caacatgatg
     5461 gatgttccgg taggccgtac ttctgcaaat gccattaccg gtactctgtt actgctggct
     5521 ggcggcaccg ctgaacaagt tgaacgtgcc acgccgatcc tgatggcgat gggcagtgag
     5581 ttgatcaacg caggcggtcc gggcatgggg atccgcgtta agctcatcaa caactatatg
     5641 agcatcgcgc tcaatgcgct ttcggcagaa gctgccgttt tgtgcgaagc cctgaatctt
     5701 cccttcgatg ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg ccacttcacc
     5761 acttcctggc cgaacaaagt cctcagcggg gatctttctc ccgccttcat gatcgatctt
     5821 gcccataagg atcttggcat cgcccttgat gtcgccaacc agctgcatgt gccaatgccg
     5881 ctgggggccg cctcacggga ggtttatagc caggcgcgcg cagcgggtcg cggtcgccag
     5941 gactggtccg ccattctgga acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa
     6001 atgtaacgac tggataaagg aataaatgaa tgaataagta caccatcaac gacattacgc
     6061 gcgcatcggg cggttttgcc atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt
     6121 ttgccgcggc tggggcaccc gctccggtag ccgatagcgt tttaactgat ttcaaagtta
     6181 acgcggcgaa gatcctctcg ccttatgcct cggcgattct ggtagatcaa caattctgtt
     6241 accgccaggt ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg
     6301 atgagttcat tcctggcaac ggtattccgg tagatagcgt ggtcattgac cgcaaaatca
     6361 atccgctaca gatcaaacaa gacggtggca aagccttaaa actgctggtg ctgtggcgta
     6421 gtgatgaaga tgcgcagcaa cgcctggata tggtgaaaga gtttaacgaa ctgtgccact
     6481 cacacggcct ggtaagcatt attgagccag tcgtccgccc accacgtcgt ggcgataaat
     6541 tcgatcgcga acaagcgatc atcgatgccg ccaaagagct gggcgacagt ggcgctgacc
     6601 tctacaaagt tgaaatgccg ctttacggta aaggtccgca acaagagctt ctcagtgctt
     6661 cacaacgtct gaatgaccat atcaatatgc cgtgggtgat cctctcttcc ggtgtcgacg
     6721 aaaaactgtt cccgcgtgcc gtacgcgtgg caatgacagc gggtgcatcg ggcttcctgg
     6781 ctggtcgtgc tgtctgggca tcggtcgtcg gtttaccaga caacgagctg atgctgcgtg
     6841 acgtttgcgc accgaaatta caacaacttg gcgatatcgt cgacgaaatg atggctaaac
     6901 gccgttaaaa tggggataca aaaatgaaat ggtttaacac tttaagtcac aaccgttggc
     6961 tggaacagga aaccgatcgc atctttgatt ttggtaaaaa ttccgtagta ccgaccggat
     7021 ttggctggtt aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca
     7081 cagctcgtat gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttact
     7141 cgttggttga tcacggtatt aaagccatga acggcgcact gcgcgataaa aaatatggcg
     7201 gctggtatgc ctgtgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac
     7261 atttctttgc tctgctgggt gctgccagcg ccgtcacaac gggtcacccg gaagcgcgca
     7321 agctgctcga ttacaccatt gaaattatcg agaaatactt ctggagcgaa gaagagcaga
     7381 tgtgcctgga gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg
     7441 ccaatatgca cgcagtggaa gctttcttaa ttgtttatga cgtaacgcat gacaaaaaat
     7501 ggctggatcg cgcgattcgc gtggcttccg tgattatcca cgacgtcgca agaaataatc
     7561 attatcgcgt taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag
     7621 ataacccggc gcaccgcttc cgcgcgttcg gaggtacacc aggccactgg atcgaatggg
     7681 gccgtttaat gctgcacatc cacgcggccc tggaagcccg ttgcgaacaa ccgccagcat
     7741 ggctgctaga agatgccaaa ggtctgttta acgccaccgt gcgtgatgcc tgggcacccg
     7801 atggtgcgga cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac
     7861 gtgtgcgttg gcctatcgtc gaagcgatgg gtacggccta cgcgctctac accgtcaccg
     7921 gcgatcgcca gtacgaaacc tggtatcaga catggtggga ctactgcatc aaatacctga
     7981 tggactacga aaatggttca tggtggcagg agctggatgc ggacaataag gtcaccacca
     8041 aagtctggga cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta
     8101 tcccgttagc gccaggcctg gctccggcag ttgcggcggg tctgctggat attaacgcga
     8161 aataactgac taaaaaaacg acatatcagg ctgcacgccg ggtatgtcgt tattcaataa
     8221 aggtacctcg tatgcaaatc accaacatgc attgcagtgg acagaccgtt tcacttgccg
     8281 cgggggatta tcacgcgacc attgtgaccg tcggtgcagg tctggctgaa ttaacctttc
     8341 agggctgtca tttagttatt ccacataaac ccgaagagat gccgctggca catctgggca
     8401 aagtccttat tccgtggccg aaccggatcg ccaacggctg ttatcgctat caggggcagg
     8461 aatatcagtt acccattaat gaacatggtt caaaagccgc tattcatggg ctgctcgcct
     8521 ggcgtgactg gcaaatcagc gaattaaccg ccaccagcgt taccctgaca gcttttctgc
     8581 cgcccagtta cggctatccg tttatgctgg cttcgcaggt tgtttattcg ctcaatgcac
     8641 gcacgggtct ttccgttgag atcgcaagtc agaatattgg tactgtgacc gcaccctatg
     8701 gtgtgggtat tcatccttat ttaacctgca acttaacatc tgttgatgaa tatctgttcc
     8761 agcttccggc aaaccaggtt tatgccgtcg atgagcacgc taatccgaca acgcttcacc
     8821 acgttgatga gctggattta aatttcacgc aggcgaaaaa aatagccgcg acaaaaatcg
     8881 accacacctt taaaaccgct aatgatctgt gggaaataac cattacccat ccacaacagg
     8941 cgctttcggt tagcctttgt tccgatcaac cctgggtaca aatttatagc ggtgaaaaat
     9001 tacagcgtca gggactggca gttgaaccta tgagttgccc gcctaacgcc tttaattcag
     9061 gtctcgactt attattactg gaaccaggga aaacgcaccg cctgtttttt aatatttacg
     9121 ggcaacacaa ttaattttat taagaaaaga acgagatcat tgtttagagt tgtatttaca
     9181 caacaggaaa tattatggac ttttttacgt tattttcacc ggatggggtc atgctggaaa
     9241 caaacgtggt atgtatcgtt atcgcgttaa tgtttcttta tacctttgtg gggatatgcg
     9301 cgggatttgg cggtggttta accactatgc cgctcgtcac gttaatgctc cccgtaaaaa
     9361 tggcaacacc actctcggta attgtcggca ccgcgaccgc cttgtacgcc acctggctgt
     9421 cacgtaaaga aacggattgg cgatcggcaa tggtactgat tattttctcg tttctgggaa
     9481 tacccgtcgg tttatatgca ctgtcgtatt tacccgacca ttttatgaaa gtgggcttag
     9541 gcggattcct tatcctgtac tccttttaca gtatgtttat tccacgcctg ccgatttatg
     9601 accgtagctg gatcgccgca cctatcggtg cagtagcagg cgcattaggc gcagctttct
     9661 caaccaacgg accaccgatg gttatctacg gtatgctgcg taacttaggc ccggcggcat
     9721 ttcgtggcac tctgaacgct ttcttcaccg caaacaacat cgcggtgatc ggcgggctgg
     9781 cgaccagcgg aattttgact gtttcgatct ttaaactggt gctgttttgt attcccacca
     9841 tgatcctcgg ttcactggtt ggacaatatg tgcataaacg tatttcagtc aaagtgttcc
     9901 gtgtgatggt ctttttattg ctgattgcct ccggttccat gttgataaaa ggcgcgctgg
     9961 gaattcccgc cgctgccgca cttctacctc cgtttacctt gttagttata ctgcaactgg
    10021 tatttggtaa aaaagtggcg gcaatccgtc aggctgaagc aagtaaataa tcacttatgc
    10081 gggtttaaat ttatatccgc acacattctt taattaatta aacctgtaat aacaggatgg
    10141 catgaaatat tagctttata ctctatggag attatttatg gatacgccac gcccacaatt
    10201 accagatttt gaatttcatc agaataacga cagttttacc ctacattttc gacaacgtct
    10261 tattttaacc catagcaaag ataatccttg tttatggatt ggctcaggta tagcggacat
    10321 cgatatgttc cgcggcaatt tcagcattaa agataaacta caggagaaaa ttgcgcttac
    10381 cgacgccatc gtcagccagt caccggatgg ttggttaatc catttcagtc gtggttctga
    10441 cattagcgcc acgctgaata tctctgccga cgatcagggc cgtttattgc tggaactaca
    10501 aaacgacaac cttaaccaca accgcatctg gctgcgcctt gccgctcaac cagaggacca
    10561 tatctacggc tgcggcgaac agttttccta cttcgatctg cgcggcaaac cgttcccgct
    10621 atggaccagt gaacaaggcg ttggtcgcaa caaacaaacc tatgtcacct ggcaggccga
    10681 ctgcaaagaa aatgcgggcg gcgactatta ctggactttc ttcccacagc ctacgtttgt
    10741 cagcacgcag aagtattatt gccatgttga taacagttgc tatatgaact tcgactttag
    10801 tgccccggaa taccatgaac tggcgctgtg ggaagacaaa gcaacgctgc gttttgaatg
    10861 tgctgacaca tacatctccc tgctggaaaa attaaccgcc ctgctgggac gccagccaga
    10921 actgcccgac tggatttatg acggagtaac gctcggcatt cagggcggga cggaagtgtg
    10981 ccagaagaaa ctggacacca tgcgtaacgc gggcgtgaag gtcaacggca tctgggcgca
    11041 ggactggtcc ggtattcgta tgacctcttt tggcaaacgc gtgatgtgga actggaagtg
    11101 gaacagcgaa aactacccgc aactggattc gcgtatcaag cagtggaaca aagagggcgt
    11161 gcagttcctt gcctatatca acccgtatgt tgccagtgat aaagatctct gcgaagaggc
    11221 ggcaaagcgc ggttatctgg caaaagatgt cgccggtggc gactatctgg tggagtttgg
    11281 cgagttctac ggcggcgttg tcgatctcac caatccggaa gcctacgcct ggttcaagga
    11341 agttatcaaa aagaacatga ttgaactcgg ctgcggcggc tggatggctg acttcggcga
    11401 gtatctgccc accgacacgt acctgcataa cggcatcagc gcggaaatta tgcataacgc
    11461 ctggcccgcg ctgtgggcga aatgtaacta cgaagccctt gaagaaacgg gcaagctcgg
    11521 cgagatcctc ttctttatgc gcgccggttc taccggtagc cagaaatact ccaccatgat
    11581 gtgggcgggt gaccagaacg tggactggag tctggacgat ggcctggcgt cggttgtccc
    11641 ggcggcgcta tctctggcaa tgaccggaca tggcctgcat cacagcgaca ttggcggtta
    11701 taccaccctg tttgagatga agcgcagcaa agagctgctg ctgcgctggt gcgatttcag
    11761 cgccttcacg ccgatgatgc gcacccacga aggtaaccgt cctggcgaca actggcagtt
    11821 tgacggcgac gcggaaacca tcgcccattt cgcccgtatg accaccgtct tcaccaccct
    11881 gaaaccttac ctgaaagagg ccgtcgcgct gaatgcgaag tccggcctgc cggttatgcg
    11941 cccgctgttc ctgcattacg aagacgacgc gcagacctat tccctgaaat atcagtacct
    12001 gttaggtcgc gacattctgg ttgctccggt acacgaagaa ggtcgcagcg actggacgct
    12061 ctatctgcca gaggataact gggtccacgc ctggaccgga gaaaccttcc acggtggaga
    12121 aataactgtt gaagcgccca tcggcaagcc gccggtcttt tatcgcgccg acagcgaatg
    12181 ggctgcactg ttcgcctcgt taaaaaacat ctaatcatgc ccgcccgtgg ggaaactcac
    12241 gggcaaatgg agaataacga tgagtcacat cacaacggaa gatccggcaa ctttgcgcct
    12301 gccctttaaa gagaaactct cttacggtat cggcgatctg gcctctaaca tcctgctgga
    12361 tattggtacg ctttatcttt tgaagtttta taccgacgtt ctggggctac ctggcaccta
    12421 tggcggcatt atctttttga tctcgaaatt ctttaccgcc tttaccgata tgggaaccgg
    12481 catcatgttg gattcccggc gtaagattgg cccgaaaggt aagttccgcc ctttcatttt
    12541 gtacgcgtca ttcccggtca ccttattggc aatcgctaac tttatcggca caccgttcga
    12601 tgtcactggt aaaacggtga tggccactat tctgtttatg ctctacgggc tgtttttcag
    12661 catgatgaac tgctcctatg gcgcgatggt gcctgctatt accaaaaacc ccaacgagcg
    12721 cgcatcactg gcggcatggc gtcagggagg cgctacgctg ggcctgctgc tgtgcacggt
    12781 gggattcgtg ccggttatga atcttatcga aggtaatcag caacttggct atatcttcgc
    12841 cgccacgctg ttttcactgt tcggcctgct gtttatgtgg atctgctact cgggcgtgaa
    12901 agagcgttat gtcgaaaccc aaccagccaa tccggcgcaa aagcctggcc tgctgcaatc
    12961 tttccgcgca attgccggta accgcccact gttcattctg tgcattgcca acctctgcac
    13021 tttaggggcg tttaacgtca agctcgccat ccaggtctat tacacccagt acgtacttaa
    13081 cgatcccatc ctgttgtcat atatgggatt tttcagcatg ggctgtattt tcatcggtgt
    13141 gttcctgatg cctggcgcag tcaggcgttt tggtaagaag aaggtctata tcggcggcct
    13201 actgatttgg gtgctgggcg atctgctcaa ctatttcttc agcggcggtt cggtcagctt
    13261 cgtggcgttc tcctgcctgg cattcttcgg ctcagcgttt gttaacagcc tgaactgggc
    13321 gctggtttcc gacaccgtcg agtacggcga gtggcgtacc ggcgttcgtt cggaaggaac
    13381 ggtctacacc ggcttcacct tctttcgcaa agtgtctcag gcgctggctg gtttcttccc
    13441 cggctggatg ctgacgcaaa tcggttatgt gccgaacgtg gcgcaggctg accacaccat
    13501 tgaagggttg cgccagctga tcttcatcta cccaagcgca ctggcggtag tcaccattgt
    13561 ggcgatgggc tgcttctaca gcctgaacga gaagatgtat gtccgcattg ttgaagaaat
    13621 agaagcccgt aaacgcacgg cgtaataata ataaacaatg ccctgcgggg cgttataagg
    13681 agtgattatg tctgaccata atccactgac attaaaactg aatctgcggg aaaaaatcgc
    13741 ctatggtatg ggcgacgttg gttcgaattt aatgcttagc atcggtactc tgtatctcct
    13801 caaattttat accgatgaat tagggatgcc agcttactat ggcgggatta tctttctggt
    13861 ggcgaagttt tttaccgcgt ttaccgatat gctcaccgga tttttactcg attcgcggaa
    13921 aaatattggg cctaaaggca aatttcgtcc ctttatttta tacgcagcgg ttccggcggc
    13981 gttaattgca acgctccagt ttatcgccac cacttttagt ttgccggtta aaacgacgat
    14041 tgccaccgcg ctgtttatga tgtttggact ttcatacagt ctgatgaact gctcgtatgg
    14101 tgcgatgatc ccggccatta ctaaaaaccc gaatgagcgc gcgcagctcg cggcttaccg
    14161 ccagggtggt gcaaccatag ggctattgat ttgtaccgtg gcgtttattc cgttgcagtc
    14221 gcttttttct gactcaaccg tcggttatgc ctgtgcggca ctgatgttct ccattggtgg
    14281 ctttattttt atgatgctgt gctacagagg cgtcaaagag cattatgtgg acacagcacc
    14341 aaccggacat aaagccagta ttctcaaatc tttttgcgcg atatttcgga atccgccatt
    14401 gctggtttta tgcattgcta acttgtgtac cctggcggca tttaatatca aactggcgat
    14461 tcaggtctat tacacccagt atgtgctgaa tgatattaat ttattgtcgt ggatgggatt
    14521 cttcagtatg ggatgcatcc tcgtcggcgt attactggtg ccattaactg taaaatgttt
    14581 tggtaaaaaa caggtttatt tagctggcat ggtgctatgg gcggttggtg atatcctgaa
    14641 ttatttctgg ggaagtaact ctttcacttt cgtcatgttc tcttgtatcg ccttttttgg
    14701 cacggccttt gtaaacagcc tgaactgggc actggtgcca gataccgtag attacggtga
    14761 atggaaaacc ggtattcgtg ccgaaggttc tgtttatacc ggttatacct tctttcgtaa
    14821 aatttctgcc gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa tcggttatgt
    14881 tcccaacatc gcgcaaagtg atgcgacatt acagggtttg cgtcagctca tttttatctg
    14941 gccttgtgca ctggcaatta ttgctgcatt gacgatggga ttcttttaca cactcaatga
    15001 aaaacggttt gcgttgatta ttgaggaaat caaccaacgc aaaaataaag agatggcgac
    15061 agaagaaaaa acggcttccg taacgttata aatgtcaata gccgctattt ccatcctggt
    15121 gggtggcggc ctccctacgt ttaaaaatgg acttattatg aaaaatatta atgcaataat
    15181 tttattatct tctctgacct ctgcctcagt attcgctggc gcatacgtcg aaaacaggga
    15241 ggcatataat ctcgcctccg atcagggcga ggttatgcta cgtgtgggtt ataacttcga
    15301 tatgggcgcg ggtattatgt taaccaatac ctacaccttt cagcgagaag atgaactaaa
    15361 acatggatat aacgaaatcg aaggttggta tccgttattt aaaccaaccg ataaattaac
    15421 catccagccc ggtggcttaa ttaatgataa gagtatcggt tcgggtggtg ctgtttatct
    15481 ggatgttaac tacaaattta ctccgtggtt taacctgaca gtgcgcaacc gttttaacca
    15541 taataactac agctcaaccg atttaaacgg tgatctggat aataatgaca gctacgagat
    15601 tggcaactac tggaacttta ttattaccga taagttctca tatacctttg agccgcatta
    15661 tttttataac attaatgatt tcaatagcgg caatggcacg aagcatcact gggaaatcac
    15721 taacaccttc cgttaccgca ttaacgagca ctggctgccc tatattgaat tgcgttggtt
    15781 agatcgcaac gtggaaccgt accaccgcga gcaaaaccag attcgtatcg ggacgaagta
    15841 tttcttctga tgtgaaactg ccggatgcgg cgtaaacgcc ttatccggcc tacggttgat
    15901 acggttttcg ttggccggat taagtacatc cggccattaa ttaagcttca gccgccagca
    15961 tctctttctt acgttggcta atacgcttaa ccagcattac ggaaaccacc gcaccacaga
    16021 atgcgaagca ggccataatg ccaaacacga ttttgtagcc aataatcccc ggatttaaat
    16081 ccagaatgta gccatacaga ctgaagcaga acatcgccgg ggcgtaacca ataaagctac
    16141 ccaacgccat cgccgcgcct gttttatttt cagcaatttt tgcttcgccg ataggtgcaa
    16201 aaaatacggc tcgctgtgta aagactatcg cgccaaagcc cagcgtacat gccatcccta
    16261 aatagaccgg catactttcg tgcggcagca taatcaacag tacgagcgca gcggtactga
    16321 tgataaaggt gtagcataga tatttactcg gcgatttcag gattttatct gaaatcatgc
    16381 caccaatcgg tccaccaatc attttcagac agtattggtt aatgatgccg taagccccca
    16441 ccagcgcaac gggcaataga tagatgtttt tcaggaatgg aatgaagaat gtcaggccac
    16501 agtaaaccgc gtaaacgaag aagacgttga aagcgataag ccagatagtt ttgtctttca
    16561 gcaccgaggt catactggtg ttttgcgatg ccccgtcttc ttttttcacc tcaacggacg
    16621 gtgcctcttc tttgtcattc aggacaaaga aaatagtaat gcctacggca atcacaataa
    16681 gggaatagaa ccagatgcct gctttaaaac ctaataagcc actgccaaac caggtaaata
    16741 ctgccaacgc agaaaatgcc accacggtat cgacaatgcc acgccctgtt tcgaagaagc
    16801 caaacaaccg accttgttgt tcgctattgc ccaatcgact taccgacttg agcaagaccg
    16861 gccagttcat catgtcgcaa gtaacgccaa atagcgccca gacaaagagg atgccccaat
    16921 accccggcat tgtcgtcaaa taaacaccca gtaatcctgt cgcaatgagt gagaatgaca
    16981 tggtgtatct gcgcggtagt ttatcggcaa aataaataga cagaaaaaag cccactgtgg
    17041 taacaaatga gtttaccgac atagcattac caatttgacc attggtcaaa tggaaatatt
    17101 cctgcatcgg gatataaaac gcatctttca gcgacggtaa tttataaatt gtaccgccac
    17161 acagtgttaa tagactaaat aacgcccatt tctttttcgt gagcataaca tcccctaagc
    17221 gattttaaaa atcactgttt taaaggcaat aagataccga ccattcttat ggaatggttt
    17281 tattaactgc gtatgagtta aaacttgtta gtggttcaga agatggctcg cctcttgttt
    17341 gatttgttgt agtacgttac gcacatgatt tatctgatta attgcatctt ctttttcttt
    17401 acgtaaccgc tcatccagca aatgcttttc tggcaatggt gtttcactca tctggcgata
    17461 agggatccac ggattatcat cttcgtcttc aaagcggaac gctggagcat aataatcaac
    17521 ctcttcttcc aggccatatg ccgtcacctg cggttcttca tcgccgaggc agataaggtg
    17581 tgttaataac tctttgaacg gcatatctcc ctgacctgaa atacaggctt tatgacccag
    17641 gccaccttgt tctttaacga tcaaggcatc tttgatatgg acctgggtaa tatgcggtgc
    17701 catcgttttt aaggcgtcta tgggatgctc atttgcattg atcatattcg caaaatcaaa
    17761 cagtaaggaa agagactcca tctcactttc tttgaccagc gacaccaact catgactttt
    17821 taaatcttca tgctgttcga tcgtaaaagt caggccgcta tcctgatacg cttcccgcac
    17881 ataggcaatg tcgttagcga tgatcgataa tacgtcgcgc aaattacctt cataacgtgg
    17941 ataaaaacgt acggacgatg ccccagtttt caacgcgatg gcgacggctt cgtcaataga
    18001 tgccttatct gaggcgctgg tttcaatatg aatatcaagg ttcagacggc gggctttatc
    18061 cccaaaggca gagagttctt tatcgtccat attaccaaga gaaaaacgct cgccatccag
    18121 aacatggatt ttgacgccac gtagattatt ttcacttgcg atatcaagta aatcagcagg
    18181 caacactctt tccagccgca tatttaaatg aaaggcataa gcatgtaaat aaagaggaag
    18241 agtgtcgaca cgttgtagaa tctttcttgc gttatttatt gtcaccataa aacacctaca
    18301 gattaatgat gaggtctacg gcctcaaatc gccaatattc aatatctatc tggacaatat
    18361 tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc tggcataccc gcagaaccgc
    18421 caagatactt gctgatatcg cccggcattc tgacgggttt gaaaatcaat ttttttacta
    18481 ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa tgagtggttt tcaagttgtt
    18541 caataaaacc cggagcaact tcaggattaa tatatgtttc gtgatagaaa actttatgtc
    18601 cttccagcgc cccccagccg gtgatgcgat agagttcagt tgatggtgtc actgcaatca
    18661 gtggcgcgag cgtctcggga atatcgctgg tacggttttt ctcggtaaac ccccaggaag
    18721 gctctcgccc ttgctcaatt gcggcccgct gaaagctcgc cgacagctcc ggactgtaat
    18781 taaatcgcgg ctgggtcacg aaccacccct tacgatcctt acggaagatt ttggattctg
    18841 cctcgaggtt caacaacgcc tggcgcagcg tcatacgttt aatgcccagc agttctccca
    18901 gctctctttc tgaagggagt tttccacccg gcgtggtgat gccatctttc aaccaccgat
    18961 ccagtttctc ttttgcattt tctacggtgg attgattctc cgccattgtt taatcctttt
    19021 ggtttaaacc agatgaagca tcttacgctt acgccaaaaa agaaagcaat taacggcgta
    19081 tgactttgca gataattgtg acaaagctct ctattgacgt aaccacagac ttataaggga
    19141 gatactcgca ttggtgtgcg ctcacgacag gcaaaaaaaa acctgccatc gctggcaggt
    19201 tttttatgac taaaaaacga aattaatcgt ctttcggtgc gcggttggcg cgacgacgat
    19261 cgttttccgt caggtgacgt ttacgaatac ggatagaggt cggagtcact tctaccagtt
    19321 cgtcatcatc gatgaactcc agagcttgtt ccagagtcat gcggataggc ggaaccagaa
    19381 caacggcttc gtcagtaccg gaagcacgca tgttggtcag tttcttaccg gtcaggcagt
    19441 ttacagtcag gtcgttagag cggctatgaa taccgataat ctgaccttcg taaacttctg
    19501 caccgtgacc gaggaacagc ttaccgcgat cctgcaaacc gaacagcgcg aacgcgaccg
    19561 ctttaccctg accgttagag atcagtacgc cgttctgacg ctgacccact tcacccggac
    19621 gcacgtcgtc gtagtggctg aaggtggagt acagcagacc cgtaccggaa gtcatggtca
    19681 tgaactcaga acggaagcca atcagaccac ggcttgggat cacgtagtcg agacgtacgc
    19741 ggcctttacc gtctggattc atgtttttca ggtcgccttt acgttcgccc agcgcctgca
    19801 ttacagaacc ctgatgctgt tcttcaacgt ccagagtcac gttttcatac ggctcttgtt
    19861 tacgaccgtc gatttcacgg aagataactt tcggacggga taccgccagt tcgaaacctt
    19921 cacgacgcat gttttcgatc agaacagaca ggtgcagttc gccacgacca gaaacgcgga
    19981 acgcatcggc gtcttcggtt tcttctacgc gcagcgcaac gttgtgtacc agttctttgt
    20041 tcagacgatc caggatctga cgagacgtta cgaacttacc ttctttaccg cagaacggcg
    20101 aggtgttaac gcagaagaac atagaaacgg tcggctcatc aacggagagt gccggcagcg
    20161 cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgccc aggcccgtga
    20221 tcgccacgat atcgccagct tccgccagat cggtttcgat acgttccaga ccgaggtggc
    20281 ccagcacttt accaactttc gcgttgcggg ttttgccttc gctatcgatg atagtaacct
    20341 gctggttcgg cttcacttta ccgcgcttga tgcggccaat gccgataacg ccaacatagc
    20401 tgttgtaatc gagctgagaa atctgcatct ggaacggacc gtcaaggtca acgtccggcg
    20461 caggaacgtg gtcaacaatc gcctggtaca gcggggtcat gtcttccgcc atatcttcgt
    20521 ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac gatcgggaag tccagctgct
    20581 cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg atccacaacc caatcaggac
    20641 gcgcgccagg gcggtcaact ttgttgataa caacaatcgg cttcaggccg taagcaaacg
    20701 cttttttggt tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca accaccagca
    20761 gcactgagtc taccatggac attacacgtt caacttcacc accgaagtcg gcgtgccccg
    20821 gggtatcaac gatgttgata cggtaatcat tccatttgat agcggtgttt ttcgcgagga
    20881 tggtaatccc acgctctttc tccaaatcgt tggagtccat cacgcgctct tgggtttcgg
    20941 cacgagagtc gaacgtaccg gattgttgga gcagcttgtc taccagggtg gttttaccat
    21001 ggtctacgtg cgcgatgatg gcgatattac gcaatttttc gatcacaact ttgcctcagg
    21061 cattagaaat agcgcgttat tgtacacgga ttaatcgcac tacaaaacag gatcacaaac
    21121 atcctccgca aacaagtatt gcagagtccc tttgtgatcg ctttcacgga gcataaaaag
    21181 ggttatccaa aggtcattgc accaatatgg tgcttaatgt ttccattgaa gcactatatt
    21241 ggtgcaacat tcacatcatg gtgcagccct tttgcacgat gttaagcatg ataacgcctt
    21301 ttaggggcaa tttaaaagtt ggcacagttt tcgctttatc ttttttacgg cgacacggcc
    21361 aaaataattg cagatttcgt taccacgacg acaatgacca atccaggaga gttaaagtat
    21421 gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg ttgatttgcg
    21481 cttcaccgat accaaaggta aagaacagca cgtcactatc cctgctcatc aggtgaatgc
    21541 tgaattcttc gaagaaggca aaatgtttga cggctcctcg attggcggct ggaaaggcat
    21601 taacgaatcc gacatggtgc tgatgccaga cgcatccacc gcagtgattg acccgttctt
    21661 cgccgactcc accctgatta tccgttgcga catccttgaa cctggcaccc tgcaaggcta
    21721 tgaccgtgac ccgcgctcta ttgcgaagcg cgccgaagat tacctgcgtt ccactggcat
    21781 tgccgacacc gtactgttcg ggccagaacc tgaattcttc ctgttcgatg acatccgttt
    21841 cggatcatct atctccggtt cccacgttgc tatcgacgat atcgaaggcg catggaactc
    21901 ctccacccaa tacgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag gcggttactt
    21961 cccggttccg ccggtagact ctgctcagga tattcgttct gaaatgtgtc tggtgatgga
    22021 acagatgggt ctggtggttg aagcccatca ccacgaagta gcgactgctg gtcagaacga
    22081 agtggctacc cgcttcaata ccatgaccaa aaaagctgac gaaattcaga tctacaaata
    22141 tgttgtgcac aacgtagcgc accgcttcgg taaaaccgcg acctttatgc caaaaccgat
    22201 gttcggtgat aacggctccg gtatgcactg ccacatgtcg ctgtctaaaa acggcgttaa
    22261 cctgttcgca ggcgacaaat acgcaggtct gtctgagcag gcgctgtact acattggcgg
    22321 cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac ccgaccacca actcttataa
    22381 gcgtctggtc ccgggctacg aagctccggt aatgctggct tactctgcac gcaaccgttc
    22441 tgcgtctatc cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg aagtacgttt
    22501 cccggatccg gcggctaacc cgtacctgtg ctttgctgcc ctgctgatgg ccggtcttga
    22561 tggcatcaag aacaagatcc atccgggcga agccatggac aaaaacctgt atgacctgcc
    22621 gccggaagaa gcgaaagaga tcccacaggt tgcaggctct ctggaagaag cactgaacga
    22681 gctggatctg gaccgcgagt tcctgaaagc cggtggcgtg ttcacggatg aagcgatcga
    22741 tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc cgcatccggt
    22801 agagtttgag ctgtactaca gcgtctaata gttgaagttg tactacccgg cgcaacaacg
    22861 ccgggattta gttgccgtgg aaactttcag cccatctctg catgggcttt tttctccgtc
    22921 aattctctga tgcttcgcgc tttttatccg taaaaagcta taatgcacta aaatggtgca
    22981 acctgttcag gagactgctt tatggcaaca ggcacgcagc ccgatgctgg gcagatcctc
    23041 aactcgctga ttaacagtat tttgttaatc gatgacaacc tggcgatcca ttacgccaac
    23101 cccgccgcgc aacaactgct cgcccaaagc tcccgcaaat tgtttggtac gccgttaccg
    23161 gaactgttga gctacttctc attaaatatt gagctgatgc aagaaagtct ggaggcgggg
    23221 caaggtttta ccgataacga agtgacgctg gtcatcgacg gtcgctcgca tatcctttct
    23281 gtgacggccc agcgtatgcc ggacggcatg atcctgctgg agatggcgcc gatggataac
    23341 cagcgccgct taagccagga acagctacaa catgctcaac aagttgctgc ccgtgattta
    23401 gtgcgtggcc tggcacatga gattaaaaat ccgcttggcg gtttacgtgg cgcggcgcag
    23461 ctactcagca aagcattacc tgacccgtca ctgctggaat ataccaaagt gattatcgaa
    23521 caggcggacc ggctgcgaaa tctggtcgac cgtctgttgg ggccgcagct gcccggtacg
    23581 cgcgttaccg aaagtattca caaagtagct gaacgcgtgg taacgctggt gtcgatggaa
    23641 ctgccggaca acgtgcggtt gattcgtgat tacgatccca gcctgccgga actggcgcac
    23701 gacccggatc aaattgaaca ggtcttgctg aatattgtgc gcaatgcgct acaggcgctg
    23761 gggccggaag gcggtgaaat cattctgcgt acccgcaccg cgtttcaact gaccttacac
    23821 ggcgaacgct atcggctggc ggcgcggatt gatgtggaag ataacgggcc aggcattccg
    23881 cctcatttgc aggatacgct gttttacccg atggtcagcg gccgcgaagg tggcaccggg
    23941 cttggcttat ccatcgctcg taatttgatt gatcagcatt caggcaaaat tgaatttacc
    24001 agttggccag ggcataccga gttctcggtt tacctgccta tcaggaaata aaggtgacgt
    24061 ttatgcaacg agggatagtc tgggtagtcg atgacgatag ttccatccgt tgggtgcttg
    24121 aacgtgcgct cgctggagcg ggtttaacct gtacaacatt tgagaacggc gcggaggtac
    24181 tggaggcgct ggcgagcaaa acgccggatg tgctgctttc agatatccgt atgccgggaa
    24241 tggacgggct ggcactgctc aagcagatta aacagcgcca tccgatgctt ccggtcatca
    24301 ttatgaccgc acattccgat ctggatgctg ccgtcagcgc ctatcaacaa ggggcgtttg
    24361 attatctgcc caaaccgttt gatatcgacg aagccgtggc gctggttgag cgcgccatca
    24421 gtcattacca ggaacagcag cagccgcgta atgttcagct taacggccca acgaccgata
    24481 tcatcggcga agcgccagcc atgcaggacg tgttccgtat tatcggtcgg ctttcgcgtt
    24541 cttctattag cgtgctgatt aacggcgaat ccggcaccgg taaagaactg gtcgctcatg
    24601 ccctgcatcg ccacagtccg cgagccaaag cgccatttat cgctctgaat atggcggcta
    24661 tcccgaagga tttgatcgaa tcagaactgt ttggtcacga gaaaggcgcg tttaccggcg
    24721 cgaataccat tcgtcagggg cgttttgaac aggctgatgg cggtacatta tttctcgatg
    24781 aaattggcga tatgccgctg gatgtgcaga cgcgtttgct gcgcgtgctg gcagacggtc
    24841 agttttaccg cgttggcggc tatgcgccgg tgaaagtgga tgtgcggatt atcgctgcca
    24901 ctcaccagaa tctcgaacag cgggtgcagg aaggtaagtt ccgtgaggat ctgttccacc
    24961 gcctgaacgt tatccgcgtt catctgccgc cgctgcgtga gcgtcgggaa gatattcccc
    25021 gcctggcgcg ccatttttta caggttgccg cgcgagaact gggcgtagaa gcgaagttgc
    25081 tgcatccgga aaccgaagcc gcgttgacgc gtctggcgtg gccaggcaac gtgcgccagc
    25141 tggaaaacac ctgccgctgg ctaacggtaa tggctgccgg acaggaagtg ttgattcagg
    25201 atttgcctgg cgaactgttt gaatcaacgg ttgcggagag tacttcgcaa atgcaaccgg
    25261 acagttgggc gacgcttctt gcgcagtggg cagacagagc gctacgttcc ggtcatcaaa
    25321 atctgctttc cgaagcgcag ccagagctgg agcggacgtt actgacgacc gcgttgcgac
    25381 atacgcaggg gcataaacag gaggcggcgc ggctactcgg ctggggccgc aacaccctga
    25441 cgcgtaagtt aaaagagctg gggatggagt gattcacggc ttgtgtgtaa agattgatta
    25501 ttgagcgcaa attgctggta ttttgcgctt tactgttccg ataagttcag tatgatcttg
    25561 cccggaaaac ggggagagtc attatgctgg aatcaataat taatctggta tcgagtggcg
    25621 cagttgacag ccacacaccg caaactgctg ttgctgccgt gctgtgtgcc gcgatgattg
    25681 ggctgtttag ttgagtttca gattgctgat gaagtgctct ttgttcatgc cagatgctgc
    25741 gtgaacgcct tatccggcct acaaaagcaa tattttcaac aggttttgta ggcctgataa
    25801 gcgtagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
    25861 gtaaacgaag cgccattcac tatttaaatc acccgagaga actgctgcat ccgcgctttc
    25921 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
    25981 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
    26041 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaataggg
    26101 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    26161 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatccac ttgctgatag
    26221 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    26281 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa attacgatgc
    26341 agcacgcctt cacgctgggc caccgccagc tcgtcatccg gacgggcaaa gtgatccata
    26401 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    26461 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    26521 ggcagatgcg catagttaaa gacgctcaga cggtcagggt ttagctccgc cacgcgtttc
    26581 agggtaaagg cgaaactttc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    26641 gtggaggtaa agccaatctc acgcgcatgg ttaagcagcg caaagataaa ctcttcatcc
    26701 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catactcagg
    26761 cgattaaagc cctcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    26821 acttcgatcg aaatctccgc atcggcattg aactggaagt tttcgcgcag cagcttcatc
    26881 aggcggctga tttgcgcttt attaagatac gtcggcgttc cgccgcccca gtgcagctgg
    26941 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    27001 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcagcc gcagaagtaa
    27061 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    27121 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    27181 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    27241 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    27301 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    27361 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    27421 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    27481 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    27541 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggca ctcagggttt
    27601 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttct agtaattcca
    27661 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    27721 tttcagtcac gcccaatgga ataggggttt tactgccaat acgtggatct ttcggtgcgt
    27781 tctggccttt gctgcctgac gtgttgtttc cgcccgctgc gcggctgccc ggcgcatgac
    27841 cacgacgttt tttctgacgc ttgcggtcac gagcttcctg attcagctct tcgcgcgttt
    27901 tgcgacgcgc ttttgcatga cctttgctgc gtgatggtga agatggtttc ataatggttc
    27961 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    28021 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    28081 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    28141 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    28201 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    28261 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    28321 cattttcaga gttttatata aaagcaatta actgatttat atagcttatt tgacatgcat
    28381 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    28441 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    28501 gccgcaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    28561 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    28621 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    28681 aaaagcctgg ctcgtacctc aaaaacccct ggacgcaccc agcttatcaa cctgtttgaa
    28741 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    28801 gagatgaagc gcaaatggca gcgcgcgctc ggcgagtacc tcgaaaaacg tcagagccta
    28861 caaggtctgg tagtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    28921 attgagtggg cggtagacag caatatcgcc gtgctggtac tgctgaccaa agcggacaaa
    28981 ctggcaagcg gcgcacgtaa agcgcaattg aatatggtgc gtgaagcggt gctggcgttt
    29041 aacggtgatg tgcaggttga aacgttttct tcgctgaaga aacaaggcgt ggacaagctg
    29101 cggcagaaac tggatacctg gtttaacgag atgcagcctg tagaagaaac gcaggacggc
    29161 gaataatttt cttaccttaa tgcttatgcc ggatgtggcg tatccggccc ataaattcaa
    29221 tagattacaa ttctaccgtt ggcctgataa acatggtgca tcaggcatta cggatctttc
    29281 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    29341 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    29401 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    29461 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    29521 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    29581 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    29641 cttcgcaacg gcatcgacat catctctatg aacttcaaat accagttcat cgtgtacctg
    29701 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    29761 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    29821 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    29881 cagcgtttca acgtagccct gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    29941 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    30001 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    30061 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    30121 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttgtcacg
    30181 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    30241 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    30301 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttacag cctggtgata
    30361 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    30421 cgatttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    30481 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    30541 cggtttaatg ccctgttttt caaacagaat ggtttgtaac tgcttggtgg aagaaaggtt
    30601 aaattcctca cctgcaattt catgcgcttt cttttccagc tcagccagac gaagggtgag
    30661 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    30721 tgaaagtact ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    30781 tttttgcaga tccggccaca ttttcagatg caactgcaag gtgacatcag catcttcggc
    30841 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaaa gtcagttgat ttttgccttt
    30901 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    30961 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    31021 aatcccacgc agctcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    31081 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    31141 gcgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    31201 gcctggctcg atagcaaaag aaagcccgac caggttagct gagatgttat caaggctgtc
    31261 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    31321 tgtttcttca tcaaggatgg tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    31381 tggtgcttcg tctgcaacac tggtttcctg tggcttcgcg gctggttttg cccctttggc
    31441 ctgtaaccat ttgcccgctt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    31501 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    31561 cagttcaacg tcggttttaa tcgtcgccag ctggtatgag agataagcaa cttctttgtt
    31621 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    31681 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    31741 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    31801 atcgatgatc agttctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    31861 ggtattcgtc atggtgttga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    31921 atcgccagtg ctgatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    31981 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    32041 catcgcgtgc aagggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    32101 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    32161 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    32221 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    32281 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    32341 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggataaaa acatgagttt
    32401 ttcaccgctt ttcgtgaaaa tcttttgtag atcttctgga tcgctcgcaa atcgtcatgt
    32461 ggataacttt gtgcataa
//
LOCUS       DAZKFZ010000031        32417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000031 DAZKFZ010000000
VERSION     DAZKFZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(127..3276)
                     /gene="acrB"
                     /locus_tag="ACHOQW_001371"
     CDS             complement(127..3276)
                     /gene="acrB"
                     /locus_tag="ACHOQW_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP9572467.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3299..4492)
                     /gene="acrA"
                     /locus_tag="ACHOQW_001372"
     CDS             complement(3299..4492)
                     /gene="acrA"
                     /locus_tag="ACHOQW_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP9572468.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4634..5281
                     /gene="acrR"
                     /locus_tag="ACHOQW_001373"
     CDS             4634..5281
                     /gene="acrR"
                     /locus_tag="ACHOQW_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP9572469.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5409..8771
                     /gene="mscK"
                     /locus_tag="ACHOQW_001374"
     CDS             5409..8771
                     /gene="mscK"
                     /locus_tag="ACHOQW_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP9572470.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8810..8974)
                     /gene="rsmS"
                     /locus_tag="ACHOQW_001375"
     CDS             complement(8810..8974)
                     /gene="rsmS"
                     /locus_tag="ACHOQW_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP9572471.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(8988..9515)
                     /gene="priC"
                     /locus_tag="ACHOQW_001376"
     CDS             complement(8988..9515)
                     /gene="priC"
                     /locus_tag="ACHOQW_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP9572472.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9585..9962
                     /gene="ybaN"
                     /locus_tag="ACHOQW_001377"
     CDS             9585..9962
                     /gene="ybaN"
                     /locus_tag="ACHOQW_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP9572473.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10115..10666
                     /gene="apt"
                     /locus_tag="ACHOQW_001378"
     CDS             10115..10666
                     /gene="apt"
                     /locus_tag="ACHOQW_001378"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9572474.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10795..12726
                     /gene="dnaX"
                     /locus_tag="ACHOQW_001379"
     CDS             10795..12726
                     /gene="dnaX"
                     /locus_tag="ACHOQW_001379"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9572475.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    12059..12123
                     /gene="dnaX"
                     /locus_tag="ACHOQW_001379"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12779..13108
                     /gene="ybaB"
                     /locus_tag="ACHOQW_001380"
     CDS             12779..13108
                     /gene="ybaB"
                     /locus_tag="ACHOQW_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9572476.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13108..13713
                     /gene="recR"
                     /locus_tag="ACHOQW_001381"
     CDS             13108..13713
                     /gene="recR"
                     /locus_tag="ACHOQW_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9572477.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13823..15697
                     /gene="htpG"
                     /locus_tag="ACHOQW_001382"
     CDS             13823..15697
                     /gene="htpG"
                     /locus_tag="ACHOQW_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP9572478.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15878..16522
                     /gene="adk"
                     /locus_tag="ACHOQW_001383"
     CDS             15878..16522
                     /gene="adk"
                     /locus_tag="ACHOQW_001383"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9572479.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16654..17616
                     /gene="hemH"
                     /locus_tag="ACHOQW_001384"
     CDS             16654..17616
                     /gene="hemH"
                     /locus_tag="ACHOQW_001384"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP9572480.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17613..18572)
                     /gene="aes"
                     /locus_tag="ACHOQW_001385"
     CDS             complement(17613..18572)
                     /gene="aes"
                     /locus_tag="ACHOQW_001385"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP9572481.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18724..20028
                     /gene="gsk"
                     /locus_tag="ACHOQW_001386"
     CDS             18724..20028
                     /gene="gsk"
                     /locus_tag="ACHOQW_001386"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP9572482.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20161..21837)
                     /gene="ybaL"
                     /locus_tag="ACHOQW_001387"
     CDS             complement(20161..21837)
                     /gene="ybaL"
                     /locus_tag="ACHOQW_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP9572483.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22074..23294)
                     /gene="fsr"
                     /locus_tag="ACHOQW_001388"
     CDS             complement(22074..23294)
                     /gene="fsr"
                     /locus_tag="ACHOQW_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP9572484.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23512..25164
                     /gene="ushA"
                     /locus_tag="ACHOQW_001389"
     CDS             23512..25164
                     /gene="ushA"
                     /locus_tag="ACHOQW_001389"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9572485.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25201..25680)
                     /gene="ybaK"
                     /locus_tag="ACHOQW_001390"
     CDS             complement(25201..25680)
                     /gene="ybaK"
                     /locus_tag="ACHOQW_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP9572486.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(25884..26678)
                     /gene="ybaP"
                     /locus_tag="ACHOQW_001391"
     CDS             complement(25884..26678)
                     /gene="ybaP"
                     /locus_tag="ACHOQW_001391"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP9572487.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26816..27157
                     /gene="ybaQ"
                     /locus_tag="ACHOQW_001392"
     CDS             26816..27157
                     /gene="ybaQ"
                     /locus_tag="ACHOQW_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9572488.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27372..29876)
                     /gene="copA"
                     /locus_tag="ACHOQW_001393"
     CDS             complement(27372..29876)
                     /gene="copA"
                     /locus_tag="ACHOQW_001393"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP9572489.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRAMLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30138..31070
                     /gene="glsA"
                     /locus_tag="ACHOQW_001394"
     CDS             30138..31070
                     /gene="glsA"
                     /locus_tag="ACHOQW_001394"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9572490.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31073..32365
                     /gene="ybaT"
                     /locus_tag="ACHOQW_001395"
     CDS             31073..32365
                     /gene="ybaT"
                     /locus_tag="ACHOQW_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9572491.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7846 a         8568 c         8528 g         7475 t
ORIGIN      
        1 aattttatac gtggttaata ctggttttcg tatgagatcc tgagttggtg gttcaattac
       61 tccttaatgt tcgtaggtta tgcataaaaa aggccgctta cgcggcctta gtgattacac
      121 gttgtatcaa tgatgatcga cagtatggtt gtgctcgata tcttcattct tgcggctaaa
      181 gcggcggcga accaccacaa agaataccgg aacgaagaag attgccagta ccgttgcggt
      241 caccatcccg cccattacac cggtacctac tgcgttctgc gcgccggaac cagcaccagt
      301 actgataacc agcggcataa cgccgaggat aaacgccagc gaggtcatca ggatcggacg
      361 taaacgcatc cgcaccgcat caagcgtcgc ttcaatcaga cctttacctt ctttatccat
      421 caagtctttg gcgaattcga cgataagtat cgcgttcttc gccgacaacc caatggttgt
      481 gagcaggcct acctggaagt aaacgtcatt ggtcaggcca cggaaggtgg cagccagcaa
      541 cgcaccgata acccccagcg gaacgaccag cataacggag aacggaatcg accagctctc
      601 gtacagcgcc gccagacaca ggaacacgac aatcaacgaa atcgcgtaca gtgaaggtgc
      661 ctggttgccg gagagacgtt cctgatagga catccccgtc cagtcatagc caacaccggt
      721 aggcagtttg ctcgccagtt gttccatcag ctccattgct tcaccggtac ttttacccgg
      781 tgccgcctgg cctaagattt ccatggatgg caggccgttg taacgttcca gacgcggcga
      841 accgtactcc caacgagaag aggagaacgc cgagaatggc accatctgac catcagcagc
      901 acgaacatac cagtcgccga tatcatccgg cagcatacgg tatttcgctt ctgacatgac
      961 gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc acatagctgc cgccccatgc
     1021 agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc agcgcctgcg ctttttcctg
     1081 gtcgatatca atcttaaact gcggggtatc ttccagaccg tttggacgta cgctggtcaa
     1141 catatcaggg tgcttcgctg cttctgcaag caactggtta cgcgcctgag tcagtttttc
     1201 gtgaccaagg ccagcctggt caatcagctc aaagtcaaag ccggttgcgg tacccagttc
     1261 cacgattgcg ggcaggttaa aggcgaaaac catcgcatct ttgatttgcg agaaagcgcg
     1321 tgttgcacgc atggtaatcg cttcaacttt gttttcttca cccggacgat cggcccagtc
     1381 cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt cccgcaaagc cgaagccgtt
     1441 aacggcgaac accgactcaa cgttgttctt ttctttggtc agatagtaat gcgttacctc
     1501 attgagcact ttctgcgtac gttcctgcgt tgcacctgct ggcagctgaa ccatggtcat
     1561 aaatacgccc tggtcctcat ctggcaagaa ggagcttggc agacgcacaa acagataggc
     1621 catgccgacc acgatgatca gatacagcac cagataacgc cccgtactgc gcagaatacc
     1681 gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac atgcggttaa accagccgaa
     1741 gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc ggtttcagca tggtggcaca
     1801 aagagctgga gtcaggatca acgccaccag taccgacagc gccattgctg aaacaatggt
     1861 aatagagaac tgacgataaa ttgccccagt agaaccgcca aagaaggcca tcggtacgaa
     1921 taccgccgac agtaccatcg cgataccgac cagagcgccc tgaatctgcc ccatcgactt
     1981 acgggtggct tcttttggcg gcaaaccttc ttccgccatc acacgctcaa cgttttctac
     2041 cacaacgata gcgtcatcca ccaacaggcc gatggcgagc accatcccga acattgttag
     2101 cgtgtttatc gagaagccaa aggcggcaag gacggcaaag gtcccgagca ataccaccgg
     2161 tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc aggaacagat acataaccag
     2221 gaacacgagg atgatcgctt cgaccagcgt tttaaccact tcgtgaatag agattttcac
     2281 gaacggcgtg gtgtcgtacg ggtaaacaat tttcagaccc gacgggaaga acggttccat
     2341 cttcgccagt tcagcacgga ttgccgcagc ggtatccagc gcgtttgcac cggtcgccag
     2401 cttgatcccc agaccggaag ccggttggcc gttaaactct gcgatgatgt cgtagttctc
     2461 accacccagc tcaatcttcg ccacatcacg cagcagtacg cgggaaccat cctgattcac
     2521 tttcagcagg attttgccga actcttcagt agaggtcaga cgcgtctgag caataataga
     2581 ggcgttaagc tgttggcctt tcaccggcgg tgtaccaccg agctggcccg ccgcaacctg
     2641 ggcgttctgc gctttgatgg cggtaataac atcaaccggc gttagctgga atttgttcag
     2701 ttcattcggg ttcatccaga tacgcatcgc gtactgtgaa ccgaacaact gaacgtcacc
     2761 cacgccagac gtacggctga tggcatcttt catattcgcc gccacgtagt cggagatatc
     2821 ctcctgcgtc atggtgccat cggtgttgat aacgccgaca accatcagga agctgctgga
     2881 tgatttctca atgctcaccc cttgctgctg aacttcttgc ggcagcaacg gcatcgccag
     2941 ctgcagtttg ttctgcacct gaacctgcgc gatatccgca tcagtaccag actcaaaggt
     3001 cagggtgatc tgtacggtac ccgtggagtc actgttagag gacatgtaca tcaggttatc
     3061 gataccgttc atattctgtt cgataacctg tgtcaccgtg tcctgcactg ttttcgcatc
     3121 agcgccgggg taggaggcgg agatcgttac tgccggcggt gcaatcgtag gatattgcgc
     3181 caccggcagt ttgaggatcg ccagcccccc tgccaacatg atgataatgg cgatcaccca
     3241 cgcaaaaatc gggcgatcga taaagaaatt aggcatgtct taacggctcc tgtttaagtt
     3301 aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg ctggttatta tcagcggtaa
     3361 cttcttgtgc ttttacctgg acaccaggac gcactttctg cagcccactt attactacgc
     3421 gatcgcctgc tttcagacct tctgtcacca gccacttatc gccaatagcc tggcttgcaa
     3481 cgatcggacg ggtttccact ttgtcatccg cgccaaccac cagtacggtg gcatcgccac
     3541 gcggcgtacg ggttacgccc tgttgcggga ctaaaatagc gtttggatta agcccttctt
     3601 ccagacgtgc gcgcacgaac atacccggca gcagagtgtg atccgggttc gggaagatag
     3661 cgcgtagggt gatagaccca gtggtctgat caacggtaac gtcagagaat tccagcgtac
     3721 cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga cactttggct ttgccgttct
     3781 cttgtttcag cgtgccattc gccagttcct gtttcaggcg caggaagtcg ttgctggact
     3841 gggtcacatc aacgtagatc ggatcaagtt gctgcacggt tgccagcgca gtcgcctgac
     3901 cgttctgtac caatgcgcct tccgtcacgt tcgacttacc aatgcgaccg ctaatcggag
     3961 aggtgacttt ggtgtaagcc agattgatcc gcgcagtttc aacggcagct ttcgccgcag
     4021 ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg atcgtactct tgcttactga
     4081 tgtactgagt accgagcagt ttctgataac gattcaccgt caattgcgcg atattggctg
     4141 cagcctgggc tttcgccaga tcacctttcg cactgtcgta tgtcgcctga taggtcgcag
     4201 gatcaatctg atagagagag acacctgctt cgatgtcgct accttctttg aaattacgct
     4261 tcaggataat cccgctaact tgaggacgaa cttctgcgat ccggtaggca ctggtgcgac
     4321 ccggaagctc ggttgtgatc tgcagaggtt cagttttgac tgttactacg ccaacggcgg
     4381 gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca tcctgttagg gctaagctgc
     4441 ctgagagcat cagaacgacc gccagaggcg taaaccctct gtttttgttc atatgtaaac
     4501 ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt aataaaccca ttgctgcgtt
     4561 tatattatcg tcgtgctatg gtacatacat tcacaaatgt atgtaaatct aacgcctgta
     4621 aattcacgaa catatggcac gaaaaaccaa acaagaagcg caagaaacgc gccaacacat
     4681 cctcgatgtg gctctacgtc ttttctcaca gcagggggta tcatccacct cgctgggtga
     4741 gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac tggcatttta aagacaagtc
     4801 ggatttgttc agtgagatct gggaactatc agaatccaat attggtgaac tagagcttga
     4861 gtatcaggca aaattccctg gcgatccact ctcagtatta agagagatat taattcatgt
     4921 tcttgaatcc acggtgacag aagaacggcg tcgattattg atggagatta tattccacaa
     4981 atgcgaattt gtcggagaaa tggctgttgt gcaacaggca caacgtaatc tctgtttgga
     5041 aagttatgac cgtatagaac aaacgttaaa acattgtatt gaagcgaaaa tgttgcctgc
     5101 ggatttaatg acgcgtcggg cagcaattat tatgcgcggc tatatttccg gcctgatgga
     5161 aaactggctc tttgccccgc aatcttttga tcttaaaaaa gaagcccgcg attacgtcgc
     5221 catcttactg gagatgtatc tcctgtgccc cacgcttcgt aatcctgcca ctaacgaata
     5281 accctgaatc tgactccagg atttttcctg gacattttcg tcgttgctat tctggttcac
     5341 tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt tcttcaggtt cagaaacctt
     5401 cattcatcat gactatgttc cagtattaca aacgatcacg gcattttgtt ttttcagcat
     5461 ttattgcttt tgtttttgtc ttgttatgcc agaacacggc gtttgcgcgg gcgtcatcga
     5521 atggtgatct gccgacaaaa gcggacctgc aggcgcaact tgactcacta aataaacaaa
     5581 aagatctttc tgctcaggac aaactggtgc agcaggatct gacagataca ttagccaccc
     5641 tcgataaaat cgatcgcgta aaagaagaga ccgttcagct acggcaaaaa gtcgctgaag
     5701 cgccggaaaa aatgcgccag gcgaccgcgg cgttaacagc acttagcgat gtcgataacg
     5761 acgaagaaac gcgcaaaatt ctgagcacgc tttcgttgcg ccagctggaa actcgcgttg
     5821 cccaggcgct ggacgatttg caaaacgcac aaaacgatct ggcgtcttat aacagccagc
     5881 tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc gatgtataac gcttcgcagc
     5941 agctgcaaca aattcgcagt cgtctggatg ggactgatgt cggcgagacg gccttacgtc
     6001 ccagccagaa agtgttaatg caggctcagc aggcgttgct gaatgcggag attgaccagc
     6061 agcgtaaaag cctggaaggg aataccgtct tgcaggatac cttgcaaaag caacgtgatt
     6121 acgtgacggc gaacagcgcc cgtctggagc accagttgca gctgttgcaa gaagcggtaa
     6181 acagcaagcg cctgacttta accgaaaaaa cggcgcagga agccgtctcc ccggatgaag
     6241 ccgcgcgtat tcaggctaat ccgctggtga agcaggaact ggaaattaac cagcagttaa
     6301 gtcagcgtct gattaccgcg actgaaaacg gtaatcagtt gatgcagcaa aacattaaag
     6361 tcaaaaactg gctggagcgg gcgctgcaat cggaacgcaa tattaaagag cagattgccg
     6421 tcctgaaggg cagcctgctg ttgtctcgta tcctttacca gcaacaacaa acgctgccct
     6481 cggcggatga actggaaaac atgaccaacc gcatcgcgga tttgcgtctc gaacagtttg
     6541 aagttaacca gcagcgtgat gcactcttcc agagcgatgc gttcgtcaac aaactggaag
     6601 aaggtcacac caacgaagtc aacagcgaag ttcacgatgc gttattgcaa gtggttgata
     6661 tgcgtcgcga attgctggat caactcaaca aacagttggg taaccagctg atgatggcca
     6721 ttaacctgca aatcaaccag cagcagttaa tgagtgtgtc gaaaaacctg aaatccatcc
     6781 tgactcagca aatcttttgg gtgaacagta accgtccaat ggactgggac tggatcaaag
     6841 cgttcccgca aagcctgaaa gatgaattta agtcgatgaa aattacggtt aactgggaaa
     6901 aagcctggcc cgccgttttt atcgctttcc tcgctggttt gccgctgctg ttgattgccg
     6961 ggctgatcca ctggcgtctg gggtggctga aagcgtatca acaaaaactg gcttccgctg
     7021 tgggttccct gcgtaacgac agccagctca acacaccaaa agcgatcctt atcgacctga
     7081 tccgtgcact gccggtgtgc ctgattattc tcgcggttgg cctgattctg ttgaccatgc
     7141 agctcaacat cagcgaactg ctatggtcgt tcagcaaaaa actggcgatt ttctggctgg
     7201 tgtttggcct gtgctggaaa gtgctggaga agaacggcgt tgccgtgcgt cacttcggca
     7261 tgccggaaca gcagaccagc cactggcgtc ggcaaattgt ccgcatcagt ctcgcgttgc
     7321 tgcctatcca tttctggtct gtggtggcag aactttcccc gctgcatctg atggatgatg
     7381 tgctggggca ggcgatgatt ttcttcaacc tgctgctgat tgccttcctg gtatggccaa
     7441 tgtgccgcga aagctggcgt gataaagagt cgcacaccat gcgactggtc accattaccg
     7501 tgctgtcgat tatcccgatt gcattgatgg tgctgactgc cacgggttat ttctacacca
     7561 cgctgcgcct ctccgggcgt tggattgaaa ccgtttatct ggtgatcatc tggaacttgc
     7621 tctaccagac ggtattgcgc ggcttaagcg tagcggcgcg gcgtatcgcc tggcgtcgtg
     7681 cgctggcgcg tcggcagaat ctggtgaaag agggcgcaga aggtgccgaa ccgccggaag
     7741 aacccaccat tgcactggaa caggttaacc agcagacgct gcgtattacc atgttgctga
     7801 tgtttgcgct gttcggtgtc atgttctggg caatttggtc cgatttgatc accgtgttca
     7861 gctatctcga cagcattacg ctgtggcatt acaacggtac tgaagctggt gctgcggtgg
     7921 tgaaaaacgt caccatgggt agtctgttgt ttgcgattat cgcctcaatg gtggcctggg
     7981 cgttgattcg caacctgcct ggtttgctgg aagtgctggt gctctcgcga ctgaatatgc
     8041 gccagggcgc gtcgtatgcc attactacca tccttaacta catcattatt gctgttggtg
     8101 cgatgacggt gttcggatcg ctgggcgtct cttgggataa actccagtgg ctggccgcag
     8161 cattatccgt aggtcttggt tttggtttac aagaaatttt cggtaacttc gtctccggtt
     8221 tgatcattct gttcgaacgt ccggtgcgta ttggcgatac ggtaaccatt ggtagcttct
     8281 cggggacggt aagtaaaatc cgtattcgtg cgacaacgat taccgatttc gatcgcaaag
     8341 aagtgatcat cccgaacaaa gcgtttgtta ccgagcgtct gatcaactgg tcgttgactg
     8401 acactactac gcgtctggtg atccgtctcg gcgtggccta tggctccgat ctggaaaaag
     8461 tgcgtaaagt gttactgaag gcggcgactg agcacccaag ggtgatgcac gaaccaatgc
     8521 cggaagtctt ctttacggca tttggtgcca gcacgttgga tcatgagctg cgtctgtatg
     8581 tgcgtgaact gcgtgaccgt agtcgtactg tcgatgagct gaaccgtact atcgatcagc
     8641 tgtgccgtga aaacgacatc aacattgcct ttaaccagct tgaagtgcat ctgcacaacg
     8701 agaagggcga tgaggtgacg gaagtaaaac gcgactacaa aggcgacgat ccgacgccag
     8761 cggtagggta aggacaataa agggcgacag tgatgccgcc ctttattttt tactcgcttt
     8821 gtgaagcctc atcgctctgt aacttctttt cataatcacg ctttacaata tccagcgcgc
     8881 gcagcacggt gcgggctggg atctgatttt cttccagcag cacaatcaaa tcgacagcca
     8941 gtttgacatc gtcaggggca ttttccagtg acatactctc tccattgcta gcgggttaaa
     9001 cgcgctaacc tgttttcgat tttttccagc gcatggcggc agcgtgccag gcgcgcttca
     9061 taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgccagg
     9121 cgggctctac gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
     9181 cgtttacgct gccagcgggc aatttggggt ggtgcgctat cccactcgcg tagcgaccag
     9241 gctgtggctt cacgcgctat agcctccagt tgcgccgcca gatgttctgc cagccaggcg
     9301 acttgcggca gttgctgttg ctcaactgca tgacgaagcg cagccagatt atcgcccgcc
     9361 ttgtcgagac aagcctgtag tgttgtcgca cgagtctgaa aaagatgcct gttgaaacga
     9421 gcacttagcg tggcgaactg tgacaccggg gcacaacgct gacgcagcgt agcgagctgt
     9481 ccttccagtt tttccagcag cagggcggtt ttcacgataa gacctcaatg aaaatgataa
     9541 ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt
     9601 taatcatcat tggctggctg gcggtagtgc tgggcacgct gggcgtggta ttaccggtat
     9661 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
     9721 ttcacgcctg gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac
     9781 atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg
     9841 ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg
     9901 cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
     9961 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
    10021 gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt taaaacgcgc
    10081 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
    10141 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
    10201 cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
    10261 tgagcgttac aaaaatgcgg gcattaacaa agttgtcggc accgaagcgc gtggcttctt
    10321 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
    10381 caaactgccg cgtgaaacca tcagtgaaac ttacgatctg gaatacggca ccgatcagct
    10441 ggagatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
    10501 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
    10561 ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa
    10621 acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat cgccagtctt
    10681 gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc
    10741 tgtgttagca ttaccccttc gagaatccac cttccagcgt ttcagagcct gccaatgagt
    10801 tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
    10861 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
    10921 ttttccggca cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
    10981 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
    11041 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa
    11101 gatacccgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
    11161 tatctcatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc
    11221 cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg
    11281 ccggtgacga ttttgtcgcg ctgtttgcaa tttcatctca aggcgctgga tgtcgagcaa
    11341 attcgtcatc aacttgagca catcctcaat gaagaacata tcgctcacga gccgcgggcg
    11401 ctgcaattgc tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac
    11461 caggcgattg ccagcggtga cggccaggtt tcaacccagg cggtcagtgc gatgctgggt
    11521 acgcttgacg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggc caacggcgag
    11581 cgcgtaatgg cgctgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg
    11641 gtggaaatgc tcggcctgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
    11701 ggcaacgaca tggccgccat cgagctgcgg atgcgtgaac tggcgcgcac cataccgccg
    11761 acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg
    11821 ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccgcgt
    11881 atgccgctgc ctgagccaga agtgccacgc cagtcctttg cacccgtcgc gccaacggca
    11941 gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact
    12001 gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg
    12061 cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg
    12121 aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg
    12181 ccatcggcgc tggaaaaagc gccagctaaa aaagaagcgt atcgctggaa ggcgaccact
    12241 ccagtgatgc agcaaaaaga agtggtcgcc acaccgaagg cgctgaaaaa agcgctggaa
    12301 catgaaaaaa cgccggaact ggcggcgaag ctggcggcag aagccattga gcgcgacgcg
    12361 tgggcggctc aggtgagtca actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat
    12421 gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat
    12481 ttgaacaacc gcggtgcaca gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca
    12541 acggttgaac tgactatagt tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg
    12601 cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat
    12661 aatattcaga ccctgcgtcg gttcttcgat gcggagctgg atgaagacag tatccgcccc
    12721 atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat
    12781 gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat
    12841 gcagaaaatg caggaagaga tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct
    12901 ggtaaaagtg accatcaacg gtgcacataa ctgccgtcgc gtagagatcg acccgagcct
    12961 gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca acgacgcagc
    13021 acgtcgtatt gaagaaacgc agaaagaaaa aatggcttct gtatcctccg gaatgcagct
    13081 gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg
    13141 gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg
    13201 ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg
    13261 tcggaaatcg gccactgcgc cgattgccgc accttcaccg aacaggaagt ctgtaacatc
    13321 tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgcg tggtggagag tccggcggac
    13381 atttacgcca ttgagcagac ggggcagttt tcaggtcgtt attttgtgtt gatgggacat
    13441 ctgtcaccgc tggacggcat cggtccggat gatatcggcc ttgatcgtct ggaacagcgt
    13501 ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca accctacggt tgaaggtgaa
    13561 gctaccgcta actacattgc cgagctttgc gcgcaatatg acgtggaagc cagccgaatc
    13621 gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac
    13681 tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct
    13741 cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat
    13801 ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt
    13861 gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg
    13921 tgagcttatc tctaacgcct ccgatgcggc ggacaagttg cgtttccgtg cgctctctaa
    13981 cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg ataaagacaa
    14041 gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag tgattgacca
    14101 tctggggact atcgctaaat ccggtaccaa atcattcctc gaatccctgg gttctgacca
    14161 ggcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt
    14221 ggccgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt
    14281 ctgggaatcg gctggcgaag gtgaatatac cgttgccgac atcaccaaag aagatcgtgg
    14341 tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt
    14401 gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga tcgaaaaacg
    14461 cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa attaacaaag cgcaggcgct
    14521 gtggactcgt aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat
    14581 cgcccacgat tttaacgatc cgctgacctg gagccacaac cgtgttgaag gtaagcagga
    14641 gtacaccagc ctgctgtaca ttccgtccca ggctccgtgg gatatgtgga accgcgatca
    14701 taaacacggc ctgaaactgt atgttcagcg tgtgttcatc atggacgacg cagaacagtt
    14761 catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa
    14821 cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac
    14881 caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa aataccagac
    14941 cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgctaacca
    15001 ggaagcgatc gccaaattgc tgcgttttgc ttctacccat accgattctt ctgcgcagac
    15061 cgtgtctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactacat
    15121 cactgcagac agctatgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa
    15181 aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac
    15241 tgagttcgac ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact
    15301 ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa gcactgactc cgttcatcga
    15361 ccgtgtgaaa gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac
    15421 cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga tggcgaaact
    15481 gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac tgaacccgga
    15541 tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca gcgagtgggt
    15601 agaactgctg ctggatcagg cactgctggc agaacgcggc acgctggaag atccgaacct
    15661 gtttattcgt cgtatgaacc agctgctggt ttcctgatgt aatgccggat gaccttcgtg
    15721 tcatccggca tttttctttt cattatctgc actttccgca aattatctcg ccattaaccg
    15781 tttcagcccc tggtgccttt cttgaggcaa tcgcctgatg gtggtatcgt ttatcgcttt
    15841 ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc
    15901 tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca
    15961 aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca
    16021 agcaaaagac attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt
    16081 taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg
    16141 taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga
    16201 attcgacgta ccggacgaac tgatcgttga ccgtatcgtc ggtcgccgcg ttcacgcgcc
    16261 gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggta aagacgacgt
    16321 taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtac gtaaacgtct
    16381 ggttgaatac catcagatga cagcaccgct gatcggctac tactccaaag aagcggaagc
    16441 gggtaacacc aaatacgcga aagttgacgg caccaagccg gttgctgaag ttcgagctga
    16501 tctggaaaaa atcctcggct aatcaagccg cagcagatct gctttcgggc aggtctgctc
    16561 atccacctca ggcaatacct ctcacagcaa ttagttcttc ttcctcactt ttccgctaca
    16621 attatcaaca agttgaatcg ataagaggcg gtaatgcgtc agactaaaac cggtatcctg
    16681 ctggcaaacc tgggtacgcc cgatgccccc acacctgaag cggtaaaacg ctatctgaaa
    16741 caatttttaa gcgacagacg cgtggttgat acctcacggt tgttatggtg gccattgctg
    16801 cgcggcgtga ttttgccgct gcgctcgccg cgtgtggcga agctgtatgc ctctgtctgg
    16861 atggaaggtg gctcgccgct gatggtttac agccgccagc aacagcaggc gctggcacaa
    16921 cgtttaccgg agacgcccgt agcgctggga atgagctacg gctcgccatc actggaaagc
    16981 gccgtagatg aactcctggc agagcatgta gatcatattg tggtgctgcc gctttatccg
    17041 caattctcct gttctacggt cggtgcggta tgggatgaac tggcacgcat tctggcgcgc
    17101 aaacgtagca ttccggggat atcgtttata cgtgattacg ccgataacca cgattacatt
    17161 aatgcactgg cgaacagcgt acgcgcttct tttgccaaac atggcgaacc ggatctgctg
    17221 ctgctctctt atcatggcat tccccagcgt tatgcagatg aaggcgatga ttacccgcaa
    17281 cgttgccgca caacgactcg tgaactggct tccgcattag ggatggcacc ggaaaaagtg
    17341 atgatgacct ttcagtcgcg ctttggtcgg gaaccctggc tgatgcctta taccgacgaa
    17401 acgctgaaaa tgctcggaga aaaaggcgta ggtcatattc aggtgatgtg cccgggcttt
    17461 gctgcggatt gtctggagac gctggaagag attgccgagc aaaaccgtga ggtcttcctc
    17521 ggtgccggcg ggaaaaaata tgaatatatt ccggcgctta atgccacacc ggaacatatc
    17581 gaaatgatgg ctaatcttgt tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg
    17641 ccgtcgcgaa gagcctcgtc ggcggttttc atcatccgtg aataatgcaa aaaggcgtgc
    17701 agcgtgcctg ggtagagttt gaactcacag ggctgctgat gcgccgctaa cgtctggtaa
    17761 agcagacggc tgtcatccag cagcggatcg aactccgccc cggcaataaa acagggcgga
    17821 acttcgcgag tgagatcatt attaaacaga cagtaatacg gcgactcgcg gtccgcgtcg
    17881 ttgcttaaat acgcctcttc atacatctgc aaatcctgtt gcgttaagcc atcccagaca
    17941 ccgcccaaca gacgacgagt cacggaatcc cgtaatccgt aaagcccata ccacagcaaa
    18001 acgcccgcaa ctttaccgca atcgatctgt ttatcacgca accacaacgc actggcgagc
    18061 gccagcatgg cacctgcgga atcaccggca aagccaatgc gggacatatt gatttgataa
    18121 tcctccgcct gctggtggaa ataacaacaa gcagccacaa tttcctctat cacttgcgga
    18181 aaacgcgctt caggtgaaag ggtgtaatca ataccaatca ccgtacattg gctgtagctt
    18241 gccagcaggc gcatgatgcg atcgtgggta tcgagattgc cgagaataaa accgcctcca
    18301 tgcaaataaa atagcgttgc cgggctatct ggctgcggac aaaagagacg tgtttccacc
    18361 tgcccatatt ttgttggaac catgtaagct ttggttgcca tttctggagc gcccgcattc
    18421 cagaatcggc gctcaagcgt gtaatactgt cgttgctcag caatcgttcc cgttgcgggc
    18481 cagggcggta aatccggctg aagagtattc acaacggtct tcatttcagc agaaataagg
    18541 tccagaacag gtaatttgtt ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt
    18601 gtaaggaacc actgccatga aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc
    18661 tcgcgcgctc ccgtcagatt gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca
    18721 accatgaaat ttcccggtaa acgtaaatcc aaacattact tccccgtaaa tgcacgcgat
    18781 ccgctgcttc agcaattcca gccagaaaac gaaaccagcg ccgcctgggt agtgggtatc
    18841 gatcaaacgc tggtcgatat tgaagcgaaa gtggatgatg aattcattga gcgttatgga
    18901 ttaagcgccg ggcattcact ggtgattgag gatgatgtag ccgaagcgct ttatcaggaa
    18961 ctaaaacaga aaaacctgat tacccatcag tttgcgggtg gcaccattgg taacaccatg
    19021 cataactact cggtgctcgc ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat
    19081 attgaaattg gcagctatgc ctatcgttac ctgtgtaaca cctccagccg taccgatctt
    19141 aactatctac aaggcgtgga tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc
    19201 ggggaacgta cctttgctat cagccctggc cacatgaacc agctgcgggc tgaaagtatt
    19261 ccggaagatg tgattgccgg agcctcggct ctggttctca cctcttatct ggtgcgttgc
    19321 aagccgggtg aacccatgcc ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat
    19381 aacgtaccgg tggtgctgac gctgggaact aagtttgtca ttgccgagaa tccacagtgg
    19441 tggcagcaat tcctcaaaga ccacgtctct atccttgcga tgaacgaaga tgaagccgaa
    19501 gcgttgaccg gagaaagcga tccgttgttg gcatctgaca aggcgctgga ctgggtagat
    19561 ctggtgctgt gcaccgccgg gccaatcggc ttgtatatgg cgggctttac cgaagacgaa
    19621 gcgaaacgta aaacccagca tccgttgctg ccgggcgcta tagccgaatt caaccagtat
    19681 gagtttagcc gcgccatgcg ccacaaggat tgtcagaatc cgctgcgtgt ctattcgcac
    19741 attgcgccgt acatgggcgg gccggaaaaa atcatgaaca ccaacggagc aggggatggc
    19801 gcactggcag cgttgctgca tgacattacc gccaacagct accatcgtag caacgtacca
    19861 aactccagca aacataaatt cacctggtta acttattcat cgttagcgca ggtgtgtaaa
    19921 tatgctaacc gtgtgagcta tcaggtactg aaccagcatt cacctcgttt aacgcgcggc
    19981 ttgccggagc gtgaagacag cctggaagag tcttactggg atcgttaagt tatcgtcggt
    20041 tcgtaggcca gataaggcgt tcacgccgca tctggcattt tgctctcaat gcctgatgcg
    20101 acgctggcgc gtcttatcat gcctacatat ttttcatatt ttacatccgg caaccaccgt
    20161 ttaccccgtc accacttcac ccgccggtgg cgtttccagc agttccagca tagtacgggc
    20221 aatttcacgc tcgcccatca ctacttgatt cgcaccacgt tcggtgatat acgccacttc
    20281 atcgtcataa tgggcgcggg caataatctc aatatccgga tttttcgcgc gggcagatgc
    20341 cacaatctca cccgcttcat aaccgttggg gatcgtcagg atcagccatt ttgcacattc
    20401 cagatgcgcc agttgcataa tttcttcgtt cgccgcattg cccaatactg cgcggacccc
    20461 gcgctctcgc agctcatcaa cacgggttcg tgacgtctca atcaccacca gcggaatatc
    20521 agaggcgagc aatttctccc ccagcaggct gcctacacga ccgtaaccca ccaatagcgc
    20581 atggttgcaa atatccactg ggatctgctt ctcttcttcg attgcctctt ccagcgtctg
    20641 ctcttccagc gtttcggtct tcgccagata tttctccagt agtgcgaaca gtactgggtt
    20701 gagcataatc gacaggatcg cccctgccag taccaggttt tgtccggcct gcggcagtaa
    20761 attcaatgcc attcccagtc ccgccaggat aaacgcgaac tcaccaatct gcgccaggct
    20821 ggcggcgata gttaatgccg tacgttggga gtgaccaaac agtcgcacca ggaaaaacgc
    20881 ggctaacgac ttaccaaaca gaataatcgc cagcgtcgcc agcactgcca gcggttgctg
    20941 aatcagaatt aacggatcaa acaacatccc gacggagaca aaaaacagca ccgcaaacgc
    21001 gtcgcgcaat ggcagcgtat cgtgggcggc acggtgactc agttcagact cgttcagtac
    21061 catcccggca aagaacgcac cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa
    21121 ggcaatccct aacgccagcg ccagcaccga cagggtaaac agctcgcgag aaccggttgc
    21181 cgcgctgcgt gccataatcc acggcaccag acggcgacct accagcatca taatggcgat
    21241 aaacgcgatc actttgccga tggtgatccc catatcgact gcaagagtgg caaagcccac
    21301 atcgccctgt tccatcattc ctgccactgc gggcagcaac accagcgtca gaaccattac
    21361 caggtcttcc acaatcaacc aaccgatggc gatttgccca cgctgactgt caattaattg
    21421 ccgttcttca agtgcgcgca gtaacaccac ggtactggcg gtggaaagac ataaaccgaa
    21481 cacgataccg gtcattaacg accagcccag cacggcagag agcgccatac ccagcagcgt
    21541 cgccacggct atctgggcga tcgcaccggg aatggcgatg gcctttaccg ccatcaaatc
    21601 cttgagcgaa aagtgcagac ccacgccaaa catcaacaga atgacgccca gttcagccag
    21661 ttccggcgca agcttggtat cggcaacaaa gcccggagtg aatggtcctg ccagcacacc
    21721 cgctaacaga tatcccacca gaggagaaat acgtagttta ttggccagca tgccgaggat
    21781 aaaggcgagc acaaggccgc caacaatggt ggtgataagc ggggtggcgt gatgcattcc
    21841 gtctcctttt cctggtggtt attgtccatt tttggccggg aaaaccaaaa ttacaggtaa
    21901 tagtttatga caatttcatt gatgatgttc atgaataatt attgaatttt gcagaaaaat
    21961 ggaattagct gcaaaaaagc acggatcgga aaacagaagg cggtttaaca gaggaaaggc
    22021 ttatggcgac tgtaaaacga tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct
    22081 ttatgccggt tatcaggcag gaatatggtc aacatcccca atagtggcag gaaagcacat
    22141 attttataga ctaactcgat gctggtgtga tcggcgataa gccccagaac tgccgctccc
    22201 agacctccca tgccgaaagc aaaaccgaaa aagagtccag aaaccatacc gatacgtcct
    22261 ggaagcagct cctgagcgta gaccagaatg gcagagaatg ccgaagcgag gataaatcca
    22321 ataatcaccg ttaaaacccc cgtccagtgc aggctggcgt agggtaaaat cagcgtaaac
    22381 ggcgcaacgc cgaggataga gccccaaatc acatatttcc gcccaatttt atcccctaca
    22441 ggcccgccga tcaccgtacc tgccgcaacg gcaaacagga aggcaaacag atgaagctga
    22501 gcattctgga tagataatcc gaatttttgc atcagataaa aggtgtaata gctgctgatg
    22561 ctcgccatat agaaatattt cgagaaaatg aggattaaca gaatgctgac cgccagtacc
    22621 actttgttgc gcggcagtgg attgataatc gtcgctttgg gttttccttt attcattcgg
    22681 tgctgtgccg agtaccaacg gctgatttgc gccaacacca cgatcgccag cagtgccgca
    22741 agcacaaacc aggcaacgtt gcctttgcca taaggcgcga taatcaccgc cgccagcaag
    22801 ggtcccaggg aactgccaaa gttgccgccg acctgaaaga tagattgtgc caggccatgc
    22861 cgcccgccgg aagccatacg ggccacgcga gaagattccg gatgaaagac cgatgaaccg
    22921 gtaccgacca gcgccgccgc cagcagaact gcgccaaaac tgcccgccag cgcaagcagc
    22981 accagaccac ttaaggtaaa gcacatgcca attggcaacg accacggcat cggatattta
    23041 tcggtccagt agccgaccac tggttgcagt agcgaagagg cgagctggaa ggtgagggtt
    23101 atcatgccaa tctgcataaa tgtcagagaa aattctgact gaagcagagg ataaatcgcc
    23161 agaatcagcg attggatcat gtcgttcagc agatgtgaga ggctgatagc acctaaaata
    23221 ccaaacgatg ttcgggcctt ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt
    23281 tcactcattg ccataggaaa gtcacttttt cagggttgcg atgttaagaa tgatcttatt
    23341 tgtgattatt acccgactaa catacctgta tgcgccgcct gaaggaagtc tcaacgctga
    23401 atacagaatt tctaatctgg atgcagattt atcttcaccg gacgcagact tgtctatgat
    23461 gtcgcgtcat actatttttc aacacgttga aatcaggtca gggagagaag tatgaaatta
    23521 ttgcagcggg gcgtggcgtt agcactgtta accacattta cactggcgag tgaagctgct
    23581 ctggcgtatg agcaggataa aacctacaaa attacagttc tgcataccaa tgatcatcat
    23641 gggcattttt ggcgcaatga atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg
    23701 gatggtatcc gcaaagaggt tgcggctgaa ggcggtagcg tgctgctact ttccggtggc
    23761 gacattaaca ctggcgtgcc cgagtctgac ttacaggatg ccgaacctga ttttcgcggt
    23821 atgaatctgg tgggctatga cgcgatggcg atcggtaatc atgaatttga taacccgctc
    23881 accgtattac gccagcagga aaagtgggca aagttcccgt tactttctgc caatatctac
    23941 cagaaaagta ccggcgagcg cctgtttaaa ccatgggcgc tgtttaagcg tcaggatctg
    24001 aaaattgccg ttattggtct gacgacggat gacacagcga agttaggcaa tccagaaaac
    24061 ttcaccgata ttgagttccg taagcccgcc gatgaagcga agctggtgat tcaggagctg
    24121 caacagacag aaaagccaga cattattatc gcggcgacac atatggggca ttacgataac
    24181 ggcgatcacg gttctaacgc accgggcgat gtggagatgg cacgcgcgtt gcctgccgga
    24241 tcgctggcga tgattgtcgg cggtcactcg caagatccag tctgtatggc agcggaaaat
    24301 aaaaagcagg tagattacgt tccaggtacg ccatgcaaac cggatcaaca aaacggcatc
    24361 tggattgtac aggcgcatga gtggggcaag tacgttggtc gggccgactt tgagtttcgc
    24421 aacggcgaaa tgaaaatggt taactaccag ctgattccgg tgaacctgaa gaagaaagtg
    24481 acctgggaag acgggaaaag cgagcgcgtg ctgtacaccc ctgaaatcgc tgaaaaccag
    24541 caaatgatct cgctgttatc gccgttccag aacaaaggca aagcgcagct ggaagtgaaa
    24601 ataggcgaaa ccaatggtcg tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc
    24661 aatatggggc ggttgattct ggcagcacaa atggatcgca ctggtgccga ctttgcggtg
    24721 atgagcggag gcggaattcg tgattctatc gaagcaggcg atatcagcta taaaaacgtg
    24781 ctgaaagtgc agccattcgg caatgtggtg gtgtatgccg acatgaccgg taaagaggtg
    24841 attgattacc tgaccgccgt cgcgcagatg aagccagatt caggtgccta cccgcaattt
    24901 gccaacgtta gctttgtggc gaaagacggc aaactgaacg accttaaaat caaaggcgaa
    24961 ccggtcgatc cagcgaaaac ttaccgtatg gcgacattaa acttcaatgc caccggcggt
    25021 gatggatatc cgcgccttga taacaaaccg ggctatgtga ataccggctt tattgatgcc
    25081 gaagtgctga aagcgtatat ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg
    25141 aaaggtgagg tgagctggca gtaatccgaa agtgccggat gttcgcatcc ggcacaatgc
    25201 ttaatcgcgg cgggcgatat cagcaaattt ggcatcgagg atcttcgcca gatcgcctgc
    25261 cgccagttcg atatccagtc cgcgcttgcc gccggaaaca taaatagtgg caaattcttg
    25321 tgcgggggcg tcgataatcg ttggcagacg ttttttctgc cccagtgggc taatcccccc
    25381 aaccaggtat cccgtcgaac gctgcgcgac catcggatcg gccatctcaa ctttcttggc
    25441 acccagcgct tttgctactt ttttaaggtc cagttgacct gcgaccggcg taacggccac
    25501 ggcaaggtgt ttcatatcac cgttcactgc gaccagcagc gttttgtaga cctgatccgg
    25561 attcaaacct aattttttga cgacttcatc gccaaaattg gtttcagccg gatcgtgctc
    25621 gtaggtatgg atttgaaacg aaatcttgtt tttttcgagt gatttaactg cgggtgtcat
    25681 ggtaattttc ctgccttaaa caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa
    25741 aaaaagagtc atcttgccta agagtattgg caggatggtg agattgagcg acaatcgagt
    25801 tacaccgtcg cttaaagtga cggcataata ataaaaaaat gaaattcctc tttgacgggc
    25861 caatagcgat attggccatt tttttagcgc aacatttgcg gtaaattccc ttctccatac
    25921 aggtgtagtg caccgaccgc gaccacatat cgccccggcg gcatggcgcg taatttatcc
    25981 cgccaggcga gatttcgctg atgcatcagc acatcgtaca gcgactgact gaacgtattg
    26041 ggcagcgtta tttcattatt ttgcggcggc gcattcagcc accagctcat catttgttgc
    26101 agcaaccgtg cgttggtatg ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg
    26161 tcagggagct ggagcaacat ggcaatctgg ttttccgccc cttccagttc aatcacgggt
    26221 ttatgttgtt gcttcgccgc ctgcaatagc tgataatcga taccgtattc tgcccgcagc
    26281 cccagttttt gtgcctgcgt cgcctgaaga accatcgcga tttgccacag cggttgggta
    26341 gaaaagagtg agggagaaat gcccatctcc tggctaatgt gctgcagttt ttggagttgc
    26401 tcctcgctaa tgcgctcttc cagcgcctcg caggcaggca aattagcaaa aggcgtatcg
    26461 ctggtggaaa catccgcctc gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa
    26521 cgggtgggta ggggagccat atcgtggcta cccatatgaa tactgccaat cagatgaaaa
    26581 tggcgattgc cgggaagaga gatatcaatg gcaggccagg tgtaatgatt accgcgcagc
    26641 gcggcccaaa gcgtttttac ccggtacaac agatccatac gacctccctt tgtgaaatat
    26701 catgctagcg cgcggggagg gatggcgcaa atggagttgg ggacttgttt aatgtgtttg
    26761 tatgattcag tatgttcttg tatcgctatt tacagggaag caacagttaa aaactatgaa
    26821 acaggcaaca agaaaaccga cgacacccgg agatattctt ctctatgaat atctggaacc
    26881 gctagatttg aaaatcaatg agttagcaga gttgctgcat gttcatcgta atagcgtcag
    26941 tgcactgatc aataacaatc gtaaactcac cactgagatg gcattccgtc tggcgaaagt
    27001 ttttgatacc acagtcgatt tttggctaaa cctccaggcg gcagttgatc tttgggaagt
    27061 tgaaaacaac atgcgcaccc aggaagaatt gggacggatt gaaacagtgg ctgaatattt
    27121 ggcacgccgt gaagagcgtg caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg
    27181 taggccggat aaggcgttca cgccgcatcc ggcatccgag caccattgcc tgatgcgacg
    27241 cttacgcgtc ttatcaggcc tacaaacctg tgcctgaacc gtaggccgga taaggcgttt
    27301 acgccgcatc cggcagtcat gcgtcgatgc caaatgcgcc accctaaagc agcgcatccg
    27361 caatgacgta cttattcctt cggtttaaac cgcagcaacc ggttggcgtt actcactacg
    27421 gtaatcgacg agagcgccat tgccgctccg gcaactaccg ggttaagcag tgttccagta
    27481 aacggccaca aaataccggc ggcgaccgga ataccgatac tgttgtagat aaacgcgccg
    27541 agcaggttct gcttcatgtt gcgcagcatt gcgcgggaaa tagcgagcgc atccgcaacg
    27601 cccatcaggc tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg
    27661 ccgcccattg caatgccgac atccgcctgg gccagcgctg gcgcatcgtt aatgccgtcg
    27721 cccaccattg ccacctgacg tccttcactt tgcaggcgtt taatcgcttc ggctttaccg
    27781 tccggcagca ccccggcgat cacctcatca atccctgctt ctttggcgat cgcattggcg
    27841 gtggttgggt tatccccggt caacatcacc agacgatatc ccgctttatg cagacgttgc
    27901 agcgccgcca cgctatcact acgcaacgga tcgcgtacag ccagcagggc taccgctttc
    27961 ccgtcaaccg ccagcagcac aggcgttgcc ccttgcgatg cctgagctga aatatctgct
    28021 tcaatcgctt tagtatcaac ttgctggtca tttaacagcg cctgattgcc cagcaataac
    28081 gcatgacctt cagcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc
    28141 tgcggcagct gcatatcgct tgctttatcg aggatcgccc gcgccagcgg atggctggaa
    28201 ccttgttcca gtgccgccgc cagacgcagc gcctgcgctt cgtcaaaatc agcaaatgtt
    28261 ttcactgcga caacctgcgg cttcccttcg gtcagcgtcc cggttttatc gaacactacg
    28321 gtatcgagtg tactagcgcg ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca
    28381 gccgcccgcc cgacgccgga aataatcgac atcggcgtcg ccagccccag cgcacacgga
    28441 caggcaataa tcagcaccgt ggtggcaatc accagggtat agacaatctg cggtgccgga
    28501 ccaaagaaat accagattgc cgcactgaca agcgcaataa ccactactac cggcacaaat
    28561 acggctgaga ttttatccgc cagctgaccg atttctggct tgctgctctg ggcctggcgc
    28621 accatacgaa taatccttga tagcgtggta tggctgccaa ccgcactggc gcgaaacaga
    28681 acgctaccgt cctgtaccac tgtcccggca tggacgctat cgccttcgcc tttttgctgc
    28741 gggataggtt cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctcg
    28801 ccatctactg gcacgcggtc gcccgtcgtc aggcgcagta gcatgcctgg ctgaacttcc
    28861 gccagcggca cgcttttttc accttcgtca gtaaccaggc gcgccgtcgg cggagttaaa
    28921 tcgagtaatt tctccagcgc ctttgaagaa cgctgacgtg cgcgcgcttc cagcatatga
    28981 ccgagattaa tcagaccgat aatcatcgca ctggcttcat agtaaagatg ccgcgcttcc
    29041 atcgggaacc actgcggcca caggttgacg ctcatcgaat agagccacgc cacacccgta
    29101 cccagcgcca ccagcgtatc catcgtcgcc gcaccgttca gcaggctttt ccatgcactg
    29161 cggtaaaaat ggccgccggc gaaaaccatc actgccaggg ttatcaggcc gataaccaac
    29221 cacaggctgc ggttgtcagc ggtgaccatc atgttatcgc cgatcatccc ccagaccatt
    29281 accgggatac ccacagccag tgcaacaatt gcctgccagc ggaagcgctt catcgtagcg
    29341 acggcggttt cttgctggcg ctcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg
    29401 tagcccgctt tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc
    29461 gcagtacgct ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca
    29521 ttttgtacgc gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg
    29581 tcatcgtcat cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac
    29641 gggattgatg actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc
    29701 gcttgtttga tggtttcgat cagctgttct gcactggcag tcccggtaac gtgcgcttca
    29761 gtgatagaca catccgcttg ttccacatcc gggcgctgtt cgagactttc tttcacgcgt
    29821 ttaacgcagt gaccgcagga caggccgtcc agggtcaggt cgatagtttg tgacataaaa
    29881 cactccttta agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa
    29941 ggtcaagaaa ttaataaccc aggcgagtaa aagtccgtaa agattaaaaa atcggctcga
    30001 tttgcatcag gattagacat ttatctcttt gttttcctgt agttaagttg cgggtgctaa
    30061 gttaaatcag gatgcctgaa aatcggcacc ggggtgagga attacctccc gcatctataa
    30121 aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt
    30181 acacccaatt tcactcactt aacggcggac aaaatgccga ttacattccc tttctggcga
    30241 atgtgcctgg tcaactggca gcagtggcta tcgtgaccag cgatggcaac gtctatagtg
    30301 cgggtgacag tgattaccgc tttgcgctgg aatccatctc gaaagtctgt acgttagccc
    30361 ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg
    30421 gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaaccg ctgtcgccac
    30481 tggtaaatgc tggcgctatt gccaccacca gcctgattaa cgctgaaaat gccgaacaac
    30541 gctggcagcg aattttacat atccaacagc aactggctgg tgagctggta gcgctctctg
    30601 acgaagtcaa ccagtcggaa caaacaacca acttccataa ccgggccatt gcctggctgc
    30661 tgtactccgc cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc
    30721 agtgctccac gctcatcaat actgttgaac tggcaacgct tggcgcgacg ctggcggcgg
    30781 gtggtgtgaa tccgttgacg cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc
    30841 tggccgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgtgttg
    30901 gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggagtgatgg
    30961 gaattgccgc gttctcacca ccgctggacg aagaaggcaa cagtgttcgc ggtcaaaaaa
    31021 tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa
    31081 cacggaaggt aataacggta acaaacccct cggtctatgg aacgtcgttt ccatcggcat
    31141 tggggcaatg gtgggggcgg ggatcttcgc gctgctgggg caggctgcgt tgctaatgga
    31201 agcctcgacc tgggtcgcct ttgcttttgg cggtattgtg gcgatgtttt ccggttatgc
    31261 ctatgcgcgt ctgggggcga gctatcccag caatggcggc attatcgact tctttcgtcg
    31321 cggattaggc aacggcgtct tttcgctggc gctctcgtta ctgtacctgt tgacgctggc
    31381 ggtgagcatc gccatggtcg cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga
    31441 aggcagccag gaggagcacc ttattttgct ctacgcgttg gggatcattg cggtgatgac
    31501 gcttttcaac tccttaagca accatgcggt agggcggctg gaggtgatcc tcgtcggcat
    31561 taaaatgatg atcctgttat tgctgattat tgccggtgtc tggtcgctgc aaccggcgca
    31621 tatttccgtc tctgcgcccc ccagctccgg tgcgttcttc tcctgtatcg ggataacctt
    31681 ccttgcctat gcgggctttg gcatgatggc gaacgcggcg gataaagtga aagatccgca
    31741 ggtcattatg ccacgggcgt ttctggtggc aattggcgtt accacgttgc tttatatctc
    31801 gctggcgctg gttttgctta gcgatgtatc ggcattagag ttagaaaaat atgccgatac
    31861 cgccgtagcg caggctgctt ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat
    31921 cggcgcttta ctggcgacgg cttcagccat caacgcgaac ctgttcgccg tgtttaacat
    31981 catggacaac atgggcagcg aacgcgaact gccgaagcta atgaataaat ccctgtggcg
    32041 gcagagtacc tggggcaaca tcattgtcgt ggtgttgatt atgctgatga cggcggcact
    32101 aaatttaggc tcactcgcca gcgttgccag cgccaccttt ttgatttgct atctggcggt
    32161 gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt
    32221 aggtacgttg gtgatgttgt tggtgatcgt tggctttatc tacagcctgt ggtcccaggg
    32281 cagccgtgcg ttgatatgga ttattggtgc aatcttactc agccttattg tggcaatggt
    32341 catgaagcgc aataaaaccg tataacacct ctctgcgcgc agtactgcct gtactgcgcc
    32401 tttgtcttta attcaaa
//
LOCUS       DAZKFZ010000032        32198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000032 DAZKFZ010000000
VERSION     DAZKFZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            236..1156
                     /gene="cbpA"
                     /locus_tag="ACHOQW_001396"
     CDS             236..1156
                     /gene="cbpA"
                     /locus_tag="ACHOQW_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP9572492.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1156..1461
                     /gene="cbpM"
                     /locus_tag="ACHOQW_001397"
     CDS             1156..1461
                     /gene="cbpM"
                     /locus_tag="ACHOQW_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP9572493.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1615..2214)
                     /gene="torD"
                     /locus_tag="ACHOQW_001398"
     CDS             complement(1615..2214)
                     /gene="torD"
                     /locus_tag="ACHOQW_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP9572494.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2211..4757)
                     /gene="torA"
                     /locus_tag="ACHOQW_001399"
     CDS             complement(2211..4757)
                     /gene="torA"
                     /locus_tag="ACHOQW_001399"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP9572495.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4757..5929)
                     /gene="torC"
                     /locus_tag="ACHOQW_001400"
     CDS             complement(4757..5929)
                     /gene="torC"
                     /locus_tag="ACHOQW_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP9572496.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6059..6751
                     /gene="torR"
                     /locus_tag="ACHOQW_001401"
     CDS             6059..6751
                     /gene="torR"
                     /locus_tag="ACHOQW_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP9572497.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6724..7752)
                     /gene="torT"
                     /locus_tag="ACHOQW_001402"
     CDS             complement(6724..7752)
                     /gene="torT"
                     /locus_tag="ACHOQW_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP9572498.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            7835..10579
                     /gene="torS"
                     /locus_tag="ACHOQW_001403"
     CDS             7835..10579
                     /gene="torS"
                     /locus_tag="ACHOQW_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP9572499.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10651..11724
                     /gene="yccM"
                     /locus_tag="ACHOQW_001404"
     CDS             10651..11724
                     /gene="yccM"
                     /locus_tag="ACHOQW_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9572500.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11773..11907)
                     /locus_tag="ACHOQW_001405"
     CDS             complement(11773..11907)
                     /locus_tag="ACHOQW_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9572501.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(11935..12165)
                     /gene="ymcE"
                     /locus_tag="ACHOQW_001406"
     CDS             complement(11935..12165)
                     /gene="ymcE"
                     /locus_tag="ACHOQW_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP9572502.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12140..12328)
                     /gene="ymcF"
                     /locus_tag="ACHOQW_001407"
     CDS             complement(12140..12328)
                     /gene="ymcF"
                     /locus_tag="ACHOQW_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9572503.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12339..12551)
                     /gene="cspG"
                     /locus_tag="ACHOQW_001408"
     CDS             complement(12339..12551)
                     /gene="cspG"
                     /locus_tag="ACHOQW_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP9572504.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            12837..13049
                     /gene="cspH"
                     /locus_tag="ACHOQW_001409"
     CDS             12837..13049
                     /gene="cspH"
                     /locus_tag="ACHOQW_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP9572505.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            13491..13796
                     /locus_tag="ACHOQW_001410"
     CDS             13491..13796
                     /locus_tag="ACHOQW_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572506.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            13903..14547
                     /gene="gfcB"
                     /locus_tag="ACHOQW_001411"
     CDS             13903..14547
                     /gene="gfcB"
                     /locus_tag="ACHOQW_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP9572507.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            14544..15290
                     /gene="gfcC"
                     /locus_tag="ACHOQW_001412"
     CDS             14544..15290
                     /gene="gfcC"
                     /locus_tag="ACHOQW_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9572508.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            15290..17386
                     /gene="gfcD"
                     /locus_tag="ACHOQW_001413"
     CDS             15290..17386
                     /gene="gfcD"
                     /locus_tag="ACHOQW_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9572509.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            17432..18571
                     /gene="gfcE"
                     /locus_tag="ACHOQW_001414"
     CDS             17432..18571
                     /gene="gfcE"
                     /locus_tag="ACHOQW_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP9572510.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            18559..19005
                     /gene="etp"
                     /locus_tag="ACHOQW_001415"
                     /gene_synonym="yccY"
     CDS             18559..19005
                     /gene="etp"
                     /locus_tag="ACHOQW_001415"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP9572511.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            19025..21205
                     /gene="etk"
                     /locus_tag="ACHOQW_001416"
     CDS             19025..21205
                     /gene="etk"
                     /locus_tag="ACHOQW_001416"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP9572512.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(21325..22629)
                     /gene="appA"
                     /locus_tag="ACHOQW_001417"
     CDS             complement(21325..22629)
                     /gene="appA"
                     /locus_tag="ACHOQW_001417"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP9572513.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(22709..22801)
                     /gene="cbdX"
                     /locus_tag="ACHOQW_001418"
     CDS             complement(22709..22801)
                     /gene="cbdX"
                     /locus_tag="ACHOQW_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP9572514.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(22814..23950)
                     /gene="appB"
                     /locus_tag="ACHOQW_001419"
     CDS             complement(22814..23950)
                     /gene="appB"
                     /locus_tag="ACHOQW_001419"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9572515.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(23962..25506)
                     /gene="appC"
                     /locus_tag="ACHOQW_001420"
     CDS             complement(23962..25506)
                     /gene="appC"
                     /locus_tag="ACHOQW_001420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP9572516.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(25640..26497)
                     /gene="hyaF"
                     /locus_tag="ACHOQW_001421"
     CDS             complement(25640..26497)
                     /gene="hyaF"
                     /locus_tag="ACHOQW_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP9572517.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(26494..26892)
                     /gene="hyaE"
                     /locus_tag="ACHOQW_001422"
     CDS             complement(26494..26892)
                     /gene="hyaE"
                     /locus_tag="ACHOQW_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP9572518.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(26889..27476)
                     /gene="hyaD"
                     /locus_tag="ACHOQW_001423"
     CDS             complement(26889..27476)
                     /gene="hyaD"
                     /locus_tag="ACHOQW_001423"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP9572519.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(27473..28180)
                     /gene="hyaC"
                     /locus_tag="ACHOQW_001424"
     CDS             complement(27473..28180)
                     /gene="hyaC"
                     /locus_tag="ACHOQW_001424"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP9572520.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(28199..29992)
                     /gene="hyaB"
                     /locus_tag="ACHOQW_001425"
     CDS             complement(28199..29992)
                     /gene="hyaB"
                     /locus_tag="ACHOQW_001425"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP9572521.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(29989..31107)
                     /gene="hyaA"
                     /locus_tag="ACHOQW_001426"
     CDS             complement(29989..31107)
                     /gene="hyaA"
                     /locus_tag="ACHOQW_001426"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP9572522.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            31533..31620
                     /locus_tag="ACHOQW_001427"
     tRNA            31533..31620
                     /locus_tag="ACHOQW_001427"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31567..31569,aa:Ser,seq:tga)
BASE COUNT         7866 a         8495 c         8225 g         7612 t
ORIGIN      
        1 aataataacg cactagtaaa acttaaagtg cctgtttcaa ctatcaaaaa tcgctcaccc
       61 tttttcacct gtttaaaata tgttcagcgc cccaccttga ggcgacctcc tctccgcgat
      121 gatttcaata acatattctg tgttggcata tgaaattttg aggattaccc tacacttata
      181 ggagttacct tacaggggtt ccttcaattt gtgttgattt acgcgagata acgctatgga
      241 attaaaggat tattacgcca tcatgggcgt gaaaccgacg gacgatctca agacaatcaa
      301 gaccgcctat cgtcgacttg cccgcaaata ccatcctgat gtcagcaaag aaccggatgc
      361 cgaagcccgc ttcaaagagg tcgctgaagc ctgggaagtg ttaagtgatg aacaacgtcg
      421 cgctgagtat gatcagatgt ggcaacatcg caacgatccg caatttagcc gtcagttcca
      481 gcatggcgac ggtcagagtt ttaacgccga agattttgac gatatcttct cgtcaatttt
      541 cggtcagcat gcccgccaga gccgtcaacg ccccgccgca cgcggccacg atattgaaat
      601 cgaagtggcg gtattcctcg aagaaacgct tactgagcat aagcgtacca tcagctataa
      661 cctgccggtt tataacgcct ttggcatgat cgaacaggaa atcccgaaaa cgctgaatgt
      721 gaagatcccg gcaggcgtcg gcaatggtca acgcatccgc ctgaaaggcc aggggacgcc
      781 gggcgaaaac ggcggtccaa atggcgattt gtggctggtg attcatattg cgccacatcc
      841 gctgtttgat attgtcggcc aggatctgga aattgtggtg ccggttagcc cgtgggaagc
      901 ggcgctgggt gctaaagtca ccgttccaac actgaaagaa agcattttgc tgactatccc
      961 gcctggcagc caggccgggc aacgattgcg cgttaaaggc aaaggtctgg tgagcaaaaa
     1021 acagaccggc gatctgtatg cggtactgaa aatcgtgatg ccgccgaaac cggatgaaaa
     1081 cactgccgcg ctgtggcagc aactggcaga cgcccagtcg tcttttgatc cacgtaaaga
     1141 ttgggggaaa gcataatggc taatgttacg gtgactttta ctattaccga attttgcctg
     1201 cataccggca tctctgaaga ggaattgaat gaaattgtcg gtttgggggt ggttgaaccg
     1261 cgtgagatcc aggaaacaac ctgggtattt gacgaccatg ccgccatcgt ggtgcaacgc
     1321 gcggtgcgcc tgcgtcatga actggctctg gactggccgg ggatcgcggt ggcgctgacg
     1381 ttaatggatg atattgcgca cctgaagcag gaaaaccgcc tgctgcgcca gcggctttcc
     1441 cggtttgtag ctcatccgtg atgtgtgttt gccggatgtg atgacgcatc ttagcctgcc
     1501 tgcaaagagt acttttcctg tgggccggat aaggcgtgtt cacgccgcat ccggcggcgg
     1561 tgccctaatg cctgatgcga cgctaaacgc gtcttatcag gctaacaaac gacgttatct
     1621 gttttggtgg tcgccctcaa ctaacaccag caataactgg cttagtgccg cgtaaaaacc
     1681 aaagctgtca tactgatggc aacgcgcaac aaactctggt aaccattgcc gcaacgccgt
     1741 gagtgttttt tgccgtaaac tgtcgattct tcgcgcagga accgtcccct ctcccagcga
     1801 aaaatgcaga tgactcaaca actcgagata gatcgccaga tgatctgtcg gttcgttgaa
     1861 attgccgctg gtttccatcc ctgcctcaac taacaagcgt ttaatctctt gctcgtcctg
     1921 tttgtaggcc gatgcatacg gcagcgccgc ttgtttgtca gtcatcagaa acaggccgca
     1981 aaagtcagcg gccagttcca gacgggcatc gtcacgtact gtcagagtgg caacacggtt
     2041 ttccagctca tccaccgccg cagtgagcgg cggttcgctt ttcagcaacg aaaaccattc
     2101 agccatctgc gcactggcga tttgcgtcag ttgttcatcg tccagttcac gggagaacaa
     2161 ctgcgccagc caggcgtaaa cacaggctat ctgttgtgct gtcagcgtgg tcataatttc
     2221 acctgcgacg cgggaacata ttcgcactgc gccaccatct ccacggggcc gttaaacgcc
     2281 gttacctgct ccactgttcc gttgcacttc tcaatttcca ccagcgtagt gtgcgcactg
     2341 gtcgcctgcg ccagctgcga agtaccgatg tcaatggtca acacgttggg gttaccgtat
     2401 ttgcacagcg caccaggctc gccgccttta tctggatcgt gccatgcccc ttcgtgaatt
     2461 cgcgccacgc cgggtgcata gcggtcagaa actactgccc ctgccaacac ctgaccgcga
     2521 gcgttaaaga cgcgtaccac atcaccgtta cgaataccgc gcgcgctggc atcctgcggg
     2581 ttaatgaaca ctggctcttt acccgctacc gtatattgct gacgcagagt ttccgactca
     2641 cataactgcg agtgaagtcg gaaatccgga tgcacagatt gcagatgcaa cggatacgtc
     2701 tgcgagccgg gcccaccgtg ggagcgttcg attttctcaa accacatcgg atgcccctga
     2761 caatcgtcgt agttcatatc ggcgatggtt ttcgaataga tctcaatcag gccactcggc
     2821 gtgcccagcg gttcgagatc tggatcttcg cggaatgcct ggtggcgaac aaacatctgc
     2881 ggatggtcaa actcgacgta ctctttgtta ttccagaagt catcaaacgc tggcaaatga
     2941 acgccgcgtc ctttgccttg ctgaacacct tcctgccaga tgcgtttcag ccagcccatt
     3001 tcgtccagcc cttcggtaaa ggcttcttcg cgattaaagc ggcggcacag ctcgcggaaa
     3061 atatcaaagt cgttgcgcgc ctcgaactgc ggcggcacga cctgtttcat ggcgataatg
     3121 ccacggttgg agtggttgcc gtactggtcg agatcgttac gctcaaactg cgtggtcgca
     3181 ggcagcacga tatcggcaaa gcggcaggtt gaggtccact ggttatctat ggcgataacc
     3241 gtttccagtt tgcgccagcc ttcaataatg cggttgatct gctgatggcg atggaagggg
     3301 ttagttccgg caaaaataca cattttcagc ggcggcagtt ttaccgattt accgttccag
     3361 ttgattactt tccccggttc gaggatcgca tcgataaaac gggcaatcgg aatggtgctg
     3421 ctgtagcctt tgtaatcact gttgtcgtga acaggcggaa tcgacgtaga gccggagaaa
     3481 ccactcagaa taacgccttt acgccccggc gtgcctgcgc cgttatagtg ccagccaaaa
     3541 ccaaagccac cacctggcag gccaatttgc cccagcatcg ccgccagaac cacaatcatc
     3601 cacgcccact gttcaccgtg ctgcatacgc tgtacgcacc agccagcaat aatctgcgtt
     3661 ctgttcgccg ccatctgccg cgccagtcca cgaatggttt cggcatcaat gccggtcagt
     3721 ttttcagccc atgcggcatc tttcggctga ccgtctttct cacccagcag atacggcagg
     3781 aactgctcaa aacccacaca gtagttagcg aggaagtttt tgtcgtacag gttttcactg
     3841 tacaacgtat gcgccagcgc cagttgcagc ggcacgtcag tttgcgggtt aaccgcaatg
     3901 tgcttcacat gctcgcgccc cagatactca tgggtggatg taacaaccgg atcgatgcta
     3961 atgacctcaa tttcaccggc ggcgactttc gctttcaact gcgcgtaata ttcataaaca
     4021 tcgtgatccg ggcaccacca gttcgcttgc tggtttttca gcaaatcgga gccccacagc
     4081 acaatggttt tgctgttctg caataccagc ggccaggagg tttgctgttc atacacttcc
     4141 atcgaaccga ctacgcgcgg caggatcacc tgcgcagcac cagtagagta atctccgccc
     4201 gtaccaacgc tattaccatg caaggcaata gctttcgcca gcatccccga agcgttatgg
     4261 aacatccccg tcgattgcca accactggcg gtcagcaagg cactcggccc gtgagttttc
     4321 tgtacgcgtt ccagttcttc atagaacatg tcgagggctt catcccagct cacacgcaca
     4381 aaacggttat caccgcgctg ggaggtgtcg ctcagatggc gcttacgcag ccagtccacg
     4441 cgtaccatcg gataacgaat acgcgccgcg ttgtgtacat gatccggcaa tccggcaatc
     4501 attttcgacg gatatttatc cagttcgaac ggttttgccg ccacaaagcg accatccttc
     4561 accgtcgcgc ggatagcccc ccagtgcgac ccggtaagaa tgccctcttt cgagatgaca
     4621 gcctcagtcg ccgcttgcgc cgcagtcgca cggcgcgacg ttaacaatga cggccccagc
     4681 atcccggcga cggttaagcc gccgagttgt gccagaaaac gccgacgtga tgcctgaaag
     4741 agatcgttat tgttcattat ttttcttcct tcttatcgcc gtgagcctta cctgcggtgt
     4801 cagacgcatt catttgcaga tatttcaaca aggtgcgttc ttcacgttta tcgaggctgg
     4861 taaagccgat catgccgttg agcgtgccaa tccagccgtt agcgtcaaag tgagagattt
     4921 ccggtgcgcc gtggcactgg ttacaggtgc cgttgtacaa cgaatccgca taagcccaga
     4981 tcggtttgat atcgttcacc atgtcacctt tcttcatcca cgcggtggcc tgcaacttgc
     5041 tccactcggt attggtgtcg gcaacggtgg ttttctccag cgtttttacc tgctgctgca
     5101 catcaccacg aatcgaggca acaaagatgc gtttacctgg gaattgggtg agtacacgct
     5161 gacgtccggc gctttctgtc cagccggtaa tttcaatttg cagccagtcg ccgtcacgtt
     5221 taaggacttt cacttccgag gcaggcagca gagaaccaga ggcttcttta tcgcctttcg
     5281 ccgcataaat tggcttaata tcaatagagt acagcgtgtc accactgtca ttagcaccgg
     5341 cgcgcagctc atcgaactgc ttacggaagc cgctactcat atccggtaac tggtgggcaa
     5401 tacctttatg acagtcgatg caggattgat tatctttcgc tgccaccttc atctgacgtg
     5461 ccgcttcagg atgctgcttc gcatgatcca tcgcatcgta gttatggcag gagcgacagg
     5521 ttgccgagtt gttttctttc attcgcgccc attcacgctc ggcaagttcc gcgcgtttgg
     5581 cttcaaattt ttcaggtgta tcaatggagt gggcaataaa ggtctggtag atatcgttgc
     5641 tcgcttccag tttgcgcttc accatgcctg gaatatccgg cgggatatga cagtcatggc
     5701 attcagctcg cacgccggag gcgttctgga aatgcaccga ctgtttatat tcttcataca
     5761 ctggttgcat actgtggcaa ctgacacaaa attcggttgt gctggtgact ttgatcccaa
     5821 cgtgtggcaa tacaatcagc gcaatgccaa tcacaatccc aatagcgacc agcgccagta
     5881 ccgaccaacg agcactgggt cggcgtagcg cgttccagag tttccgcata atagcccctg
     5941 taaaattatg gtttagtgaa gcgatcttaa tgagtaaata tgaacagcgg cactggtcag
     6001 gatgaacggc ttacggcaga atatgaacag atatgaacag aatgagtaaa accctctgat
     6061 gccacatcac attgttattg ttgaagatga gccggttacc caggcgcgat tacaatccta
     6121 cttcactcag gaggggtata ccgtttccgt tacggcgagc ggtgccgggc tacgggaaat
     6181 tatgcagaat cagccggtgg atttaattct gctggatatc aacttgcccg atgaaaatgg
     6241 cctgatgtta acccgcgccc tgcgagaacg ctcaacggtg gggattattc tggttaccgg
     6301 acgcagcgat cggattgacc gtattgttgg gctggaaatg ggcgcagacg attacgtcac
     6361 caaaccgctg gaactgcgcg aactggtagt acgggtaaaa aatctgctct ggcgaatcga
     6421 cctcgcgcga caagctcaac cgtacactca ggacaactgc tatcgctttg cgggttattg
     6481 cctgaatgtg tcgcgccata cgctggagcg ggatggtgag ccgattaaac tgacccgcgc
     6541 agagtatgaa atgttggtgg catttgtgac caatccgggc gaaattctca gccgtgaacg
     6601 tctgctgcgt atgctctctg cgcgtcgggt ggaaaaccct gacctgcgca ccgtcgatgt
     6661 gttaattcgt cgtttacgcc ataaactcag cgcggattta ctggtgacgc aacatggcga
     6721 aggttatttc ttagccgctg atgtgtgctg ataaaaatag accggacgaa atcccccagg
     6781 cgacagcgag cggcggatat gttcgcggtc ggcatttttc ggcgtcagaa ctaaaatcgg
     6841 tgggctgaca ttatcagaca ccgattgccc ctgtaattgc ctgatggcct gctcaactgc
     6901 cagttccccc tgccagacca tttgatcgct ggcagccata atcactcttc cccgcttcag
     6961 cccgcgatac acctgatgtg aaagataaaa cgacaccacg gtaagcggcg ttttcaggtt
     7021 acgcccttcc cccattgccg cctctgccgc aatggccgtt ccggcaacga cgtcgatttc
     7081 tggatggcgt tccagcatct cctgcaacag gttacgctgg atttcaatat cgttatcacc
     7141 aagcgcaata tcaacaatac gcaccgggct tccggcaatg gctgcgcgaa aaccctcgac
     7201 catctcctta ctgcccccgg cgttatcggg tccgggcatc aacagcacat tcagtggttt
     7261 accgtgcgcc cattgcacca gatatcgccc aggttgatag cccatctgaa accagggcac
     7321 accaacgcgg cttttcacct ggggagcatc aatagcattt accagctcga tcaccggcag
     7381 atttgctact tgcttttgca gatcgggaaa tgacgtcgtg ctactaccga gcaaaatggc
     7441 ctctgcgccc cactgtttac actggtcgat ttgtgcttgc tgggtagcca actggctgta
     7501 gccgcctgcc tccagcactt ttaaatccac accgtagcgg cgagcagcct cctgcatacc
     7561 atagttcaac gataaccaat atgaatcttt caggctggga taaagcgcgc acagtttcca
     7621 tacgcgtttg gcttttagcg gcatagaggc ttgcaccgtg aaatgctgcg catcatgcca
     7681 gcgcaacagg ttatcagccg aaaatgccgg caacatgaaa agggaaagaa gtaaaaatag
     7741 cagcacgcgc atgatagcct catcaataat aaggctttat gctagatgca ttccgctttg
     7801 cgactcaaca tttttcacct taagtgcacc gaccgtgaat ttaaccctga cccgaagact
     7861 ctggatgggc tttgccctga tggcgctgtt aaccctgacc agtaccctgg tgggatggta
     7921 caacctgcgc tttatcagcc aggtggaaaa agacaatact caggcattga ttcctaccat
     7981 gaatatggcg cgccagttga gcgaagccag cgcctgggaa cttttcgccg cgcagaacct
     8041 gaccagtgcc gataacgaaa agatgtggca ggcgcagggg cgaatgctca ccgcacaaag
     8101 cctgaagatt aatgcgttgc tgcaagcgtt acgggaacaa ggttttgata ccaccgctat
     8161 tgaacaacag gagcaggaga tctcccgttc gttacgtcag caaggggaac tggtggggcg
     8221 gcgtctgcaa ctgcgccagc aacaacagcg actcagtcag cagatagtcg ctgccgccga
     8281 tgagatcgca cgcctggcgc aaggtcaggc gaataatgcg gcaacctccg ccggagcgac
     8341 ccaggccggg atttacgatt tgatcgaaca agatcagcgt caggctgctg aaagtgcact
     8401 cgatcggctg attgatatcg atcttgagta tgttaaccag atgaatgaac tgcgccttag
     8461 cgctctgcgg gtgcagcaaa tggtgatgaa tctggggctg gagcagatcc agaaaaatgc
     8521 gccaacgctg gaaaagcagc tcaataatgc ggtgaaaatt ctgcaacgtc ggcaaatacg
     8581 cattgaagat ccgggagttc gtgctcaggt cgcaacaacg ttaactaccg ttagccaata
     8641 tagcgatttg ctggcgctgt tccagcagga cagtgaaatc agcaatcacc tacaaactct
     8701 cgcacaaaat aacatcgccc agttcgcgca gtttagcagc gaagtcagtc agctggtcga
     8761 caccattgag ctgcgtaatc agcacggact ggcgcatctg gaaaaagcca gtgcacgcgg
     8821 gcaatacagc ctgttattgc tggggatggt ttcactttgc gcactgattc tgatcctctg
     8881 gcgcgtggtt tatcgctcag tcacgcgtcc acttgccgaa caaacgcagg cgctgcaacg
     8941 gctgctggac ggtgatattg actccccttt cccggaaacc gctggcgtac gggagctgga
     9001 caccatcggg cggctgatgg atgcgtttcg cagcagtgtt catgcactga atcgccaccg
     9061 cgaacagctg gcggcgcagg tcaaagcgcg tacagctgaa ttgcaggaac tggtgataga
     9121 acaccgacaa gcacgggcgg aagcagaaaa agccagccag gcaaaatcgg cgtttctggc
     9181 ggcgatgagc catgagatcc gcacaccgct gtacggtatt ctcggcaccg cacagctact
     9241 ggcagataac cccgcactta acgcccagcg tgatgatttg cgggcaatta ccgactccgg
     9301 cgaatcgttg ctgaccatcc tcaacgatat tctcgattat tccgctatcg aagcaggtgg
     9361 caagaatgtt tcggtcagcg atgaaccctt tgaaccgcga ccgctgctgg aaagtaccct
     9421 gcaattaatg agtggacggg tgaaaggtcg cccgattcgc ctggcaacgg caattgccga
     9481 cgatgtaccg actgcgttaa tgggcgatcc gcggcgtatt cgtcaggtca ttaccaacct
     9541 gttgagcaac gccctgcgtt ttactgacga agggcagatc gttttacgta gccgtactga
     9601 tggtgagcaa tggctggtcg aagtggaaga cagcggctgc ggtattgatc ccgcgaaact
     9661 ggcagaaatc ttccagccat ttattcaggt aagtggcaaa cgcggcggca ccgggctggg
     9721 gctgactatc agtagccgtc tggcccaggc gatgggcggc gaactgagcg ccaccagcac
     9781 gccggaggtt ggaagctgtt tttgtttacg cttgccgtta cgtgttgcca cggcacccgt
     9841 gccaaaaaca gtcaatcagg cggtgcgtct tgacggttta cgtttgctgt taattgaaga
     9901 taacccgcta acccagcgaa ttaccgttga gatgctgaac accagtggtg cgcaggttgt
     9961 tgctattggc aatgccgcgc aggctttaga gacactgcaa aatagcgaac cgtttgctgc
    10021 cgcactggtg gattttgatc tgccggatgt caacggcatt acgcttgccc gacaactggc
    10081 acgacaatat ccgtcgctgg ttttgattgg ctttagcgcc catgtcattg acgaaacgct
    10141 gcgccagcgt accagttcac tatttcgcgg gattatccct aaaccggtgc cgcgtgaagt
    10201 gctcggtcag ttactggcgc actatctcca actgcaagcc aataacgatc taccactgga
    10261 tgtatcgcaa ctcaatgaag acgctcagtt aatggggacg gagaagatcc acgaatggct
    10321 ggcattattt acacaacatg ccctgccgct tctcgatgaa atcgacattg cccgcgccac
    10381 tcaggatagc gagaaaataa agcgtgccgc gcatcagcta aaaagcagtt gctcgagtct
    10441 gggaatgcgt agcgccagcc agctctgcgc acaactggag cagcagccat tatctgcccc
    10501 ccttccacac gaagaaatta cacgcagtgt tgccgctctg gaagcatggt tgaataagaa
    10561 agacctgaac gcgatttgaa tcaatatcag ccagttaaga ataactagaa tagtggcatc
    10621 tggtatgcaa cttaacggac tggcaaaatc atggcagaga aaaaaagaac ccgctggcag
    10681 cggcggccag gcacgacggg cggcaaatta ccgtggaatg actggcacaa tgccacgacc
    10741 tggcgtaaag cgacgcaatt attgctgttg gcaatgaata tttatattgc catcacgttc
    10801 tggtattggg tgcgctatta cgaaacggca ggtagtacga tatttgtcgc cagaccggga
    10861 ggtatcgaag gctggctacc gattgccggt ctgatgaatc tgaaatatag ccttgcaaca
    10921 ggtcagttac cgtccgtcca cgccgccgcg atgctgttat tggtcgcttt tatcgtcatc
    10981 agtctattac tcaaaaaggc cttttgctca tggttatgcc cggttggtac gctttctgaa
    11041 ttaatcggcg ctctcggtaa caaactgttt ggtcggcaat gtgtccttcc ccgctggctg
    11101 gatattcctc tgcgcggtgt aaagtatttg ctgttgagtt tttttctcta tatcgcgtta
    11161 ttgatgcctg ctcaggcgat tcactatttt atgttgtcgc cctacagcgt ggtgatggac
    11221 gttaaaatgc tcgatttctt tcgtcatatg gggaccgcga cattaatcag cgtgaccgtt
    11281 ttgctgattg ccagcctgtt tattcgccat gcctggtgtc gttatctttg cccatatggc
    11341 gcgctgatgg gcgtggtttc gctattatcg ccgtttaaga ttcgtcgcaa tgccgaaagt
    11401 tgtatcgatt gtggcaaatg cgcaaaaaat tgcccatcac ggatcccggt cgataaatta
    11461 attcaggtac gaacagtgga atgtaccggc tgtatgactt gcgtagagtc atgtccggta
    11521 gcctcaacat tgaccttttc actgcaaaaa cctgcggcaa ataaaaaagc ctttgcgttg
    11581 tctggctggt taatgacgct actggttctg gggattatgt ttacggtgat tggttacgca
    11641 atgtatgcag gagtatggca aagcccggtg ccggaggaat tgtaccgacg cttaattccg
    11701 caagcgccaa tgattggtca ctaaattagc aacaagcgga acatcacctc gcacggggtg
    11761 atgttcacaa aatcacatta ttttgatttt gacatcataa ctttcaagcc cggtcatttt
    11821 ttctcgcgcg gtgaagcgaa tttcagagac ttctttccct gtattcttgt tcagctcggc
    11881 gattttttgc gcgataaagt cggcgatttc cgtttcggct tgttttttaa ctcttcaata
    11941 ttcactgtta acctcttctg gtcttaaaag acagcaacac gccagtaagg tgttggttcc
    12001 cggtaccctg gtcaggtaag aaatgttata agccatctcc ccgaaagatt caataatcaa
    12061 caggttatcg cttactatac atacagcgtt gaaaaaaaat aagacagata taaaaaaggc
    12121 tccacaaggg agcctgatat tattttgtga aatccagcgg tgcatggtca gtgcgtattt
    12181 gcagcgcgac gttatcaaag gtgatcatcg tcgatttaca aaaaatacac ttagcgccca
    12241 aagggtttcg ctccgtcaca tcgaatgccg atgtgcggta ctgtgaacca tgacaacatg
    12301 gacagcgaaa atggagatgt tgagtaataa cggcaacctt agagcgtaac tacgttcgct
    12361 gccgcggggc cacgttgacc ctgctcaata gaaaattcaa ctttctgatt ttcgttcagc
    12421 gtgcggaatt cattgctctg gatggcggtg aaatggacga aaacgtcttt gctgccatca
    12481 tcaggagtga taaagccaaa acctttatct gcgttaaacc attttactaa accagtcatt
    12541 ttattagaca tattttactt ccttcatttt gggcctttcg gcaaacaggg ctttaataca
    12601 gaaactactt agtgttcagg agagagactc aaaagaaggg gataatgcct gataatgaga
    12661 actgctttag taaactactt tgtatgctgt ctgtctttcc aaccgacgca gctattaacg
    12721 catgataatt gagattaagc aaattttatt ttagccgtcc ggcggtctta aatatcgctg
    12781 gctttatttt attaaccccg tatagtgcag cgcagtattt tcagtaagga atttgtttgt
    12841 cccgtaaaat gacaggaatt gtcaaaacct ttgatcgcaa aagcggcaaa ggattcatta
    12901 tcccctccga cggtcgtaaa gaagttcagg tccatatttc cgcattcact ccccgcgacg
    12961 cagaagtgct tataccagga ttacgcgtgg aattttgccg cgtaaatggc ctgcgaggac
    13021 caacagcggc aaatgtttat ctctcttaat atgacgctgc ttgtttaaag ctgactggtt
    13081 ttattggcat agttcaatag tcaacaccat gcattataac acaagttact ttttcaccgg
    13141 aaaactaatc catttaaccg aatatctcta caactaatag ttataaatgc cacttaacag
    13201 cgagttatag taccacttga aactatattg ccaacaatag ttaatagctt tcttaacaat
    13261 tttcttatct ttaatttgta ttttgctaag atttatctta tgctcacagg tcgtatttcc
    13321 agaggggaat agttaacaat cttatatttc cctctgcgcg cctttctcac ccgctgtata
    13381 tagtgcaagt ggtatgagga aagggagatt tcacaaggat gttatactca aagtagacaa
    13441 tcctgatctc aagcgtacgt attgtccatg cgatgaataa aggaaaagtt atgaaacaca
    13501 agctttctgc aatccttatg gccttcatgt tgacgacgcc agctgcgttt gctgcccctg
    13561 aagcagcaaa cgggaccgag gcaacgacgg gtactactgg caccacaacg accactaccg
    13621 gtgcaaccac gacggctgct accactggtg gcgtggctgc tggcgctgtc ggcacggcga
    13681 cagttgtcgg tgtggcaacg gctgttggtg tggcgacgtt ggcagtagtt gcagcaaatg
    13741 acagtggcga tggcggttct cataacacct caaccaccac gtccactacg cgctaattct
    13801 gcttaaacgc gtcgaaatta aagttaaata atacccggtt gcgatattcg taccgggctt
    13861 tagagatttg cattttttta attaaccttt caaggattaa aggtgcgccc tcttatttta
    13921 tcgattttcg cactatttct tgcgggatgt acgcacagcc agcaaagtat ggtcgataca
    13981 tttcgcgcca gccttttcga taatcaggat atcaccgtag cggatcagca gatccaggcg
    14041 ttgccttatt ccacgatgta tttacgcctt aatgaagggc aacgaatctt tgtggtactg
    14101 ggatatatag aacaagaaca aagcaaatgg ttatcccagg ataacgccat gctggttacc
    14161 cacaatggac gtcttttaaa gaccgtcaaa cttaataata atctgctgga agtgacaaat
    14221 tccgggcagg accctctgcg taacgcgctg gcaataaaag acggcagccg ctggacgcgc
    14281 gatattctct ggagtgaaga caaccatttt cgctctgcga ccctgagttc cactttttcc
    14341 tttgctggat tagagacgct gcatattgcg ggtcgcgatg tgctgtgtaa tgtctggcag
    14401 gaagaggtga cttccacgct accagaaaaa cagtggcaaa acacattctg ggtcgattcg
    14461 gctactggcc aggttcgtca aagtcggcaa atgttaggcg caggggttat tcccgtagaa
    14521 atgacgtttc ttaaacccgc accatgaata aattacagtc gtatttcatt gccagcgtac
    14581 tttacgtaat gacaccccat gcctttgcgc aaggaacggt gactatttat ctgcctggcg
    14641 aacaacagac actttccgtt ggccccgtgg agaatgttgc ccagttagtg acacaaccgc
    14701 aactgcgcga tcgcctctgg tggcctggtg ccctgctgac cgactccgcg gtgaaagcta
    14761 aagcgcttaa agactaccaa catgtcatgg cacagcttgc ttcctgggaa gcagaagctg
    14821 acgacgatgt ggcggcaacg attaaatccg tgcgccagca gttactcaat ctcaatatta
    14881 ctggtcgctt accggtcaaa ctggaccctg attttgtacg agtggatgag aacagcaatc
    14941 ccccgctggt cgggaactat acgctctata ccgtacagcg cccggtgact attactcttc
    15001 tgggtgcggt ttctggggct gggcaattgc cctggcaggc aggccgtagc gttaccgatt
    15061 atctgcaaga tcacccccgt cttgcaggcg cggacaagaa taacgttatc gtgattacgc
    15121 ctgagggtga gactgtcgtc gcccctgttg ccctgtggaa taaaagacat gtggaaccac
    15181 cgcctggcag ccagctttgg ttaggctttt ctgcgcatgt actgcccgaa aaatatgctg
    15241 atttaaatga ccagattgtg tctgttttaa cgcaacgtgt tcctgactga tgaagaagaa
    15301 ttcttatctt ttaagctgcc tggccattgc cgtctccagt gcctgtcatg ctgaagtatt
    15361 aacctacccg gatccgctgg gttcgtcgca atcggacttt ggcggcacag gattgttgca
    15421 gatgccaaat gcgcgcatcg caccggaagg tgaattcagc gtcaactacc gggataacga
    15481 tcaataccgt ttctactcca cctccgtggc gctgttccca tggctggaag gcaccattcg
    15541 ttatacggat gtgcgcacac gtaaatatag ccagtgggaa gatttcagcg gcgatcagtc
    15601 atacaaagac aaatcattcg attttaaact tcgcctgtgg gaagaaggtt actggctacc
    15661 gcaagtggcg tttggtaaac gtgatattgc tggtacgggt ctgtttgacg gtgagtatct
    15721 ggtggccagc aagcaagcgg ggccatttga tttcaccctc gggatggcat ggggctacgc
    15781 cggtaatgcg ggcaatatta ccaacccgtt ttgccgggtg agcgataaat attgtcatcg
    15841 cgcagagtct cacgatgcgg gcgatatcag ctttagcgat atctttcgtg gcccggcttc
    15901 catctttggc ggcattgagt atcaaacgcc gtggaatccc ctgcgtctga aactcgaata
    15961 cgacggcaac aattaccaga atgatttcgc tggcaaactg cctcaggcaa gccatttcaa
    16021 cgtcggtgca gtttatcgcg ctgccagctg ggcagacctc aacctgagtt atgaacgcgg
    16081 taacacgttg atgtttggtt tcactttacg gaccaatttc aacgatctgc gccctgccct
    16141 gcgcgatacg ccaaaaccgg catatcaacc tgcgcctgaa tctgaaggat tgcagtacac
    16201 cacggtagca aaccaactta ccgccctgaa gtataacgcg ggctttgacg cgccagaaat
    16261 tcagctacgc gataagacgc tgtatatgtc tggtcagcaa tacaaatacc gtgactctcg
    16321 tgaagcggtc gatcgtgcca accggattct ggtgaataac ctgccgcaag gcgttgagaa
    16381 gattagcgtg acgcaaaagc gcgagcatat ggcgatggtg actaccgaaa ccgacgtagc
    16441 cagcctgcgc aaacagctgg caggtacagc gcctggtcaa tcagagccac tgcaacaaca
    16501 acgtgttgaa gctgaagatc tttctgcctt tggtcggggc taccgtattc gtgaagatcg
    16561 ctttagctac tctttcaacc caacactttc acagtcgctg ggcggcccgg aagatttcta
    16621 tatgttccag ctggggctga tgtccagcgc ccgctactgg tttaccgacc acctgctgct
    16681 tgatggcggt attttcacca atatttacaa caactacgac aagtttaagt cttcgctgtt
    16741 gcccgcggac tctaccctgc cccgcgtgcg cacgcatatc cgtgattacg ttcgcaatga
    16801 cgtttatctc aacaacttgc aagcgaatta ctttgccgac ttaggcaatg gtttctatgg
    16861 ccaggtgtat ggcggttatc tggaaacgat gtatgccggt gtcggttccg agctgcttta
    16921 tcgcccgcta gatgccagct gggcgctggg tgtggacgtt aactacgtga agcagcgtga
    16981 ctgggacaac atgatgcgct tcaccgatta ttccacgcca actggtttcg tgacggctta
    17041 ctggaacccg ccgacgctca atggcgtact gatgaaactt agcgttgggc aatatctggc
    17101 aaaagataaa ggggcaacga tcgacgtcgc caaacgcttt gacagcggcg tggcggtagg
    17161 ggtatgggcg gcaatcagta acgtatctaa agatgactac ggcgaaggcg gctttagtaa
    17221 aggtttttat atctcgattc cgttcgactt gatgaccatt ggacctaacc gcaaccgcgc
    17281 ggtggtttcg tggacaccat tgacgcgtga tggtggacaa atgctgtcac gcaaatacca
    17341 gctctatcca atgacggcag agcgagaagt accggttgga caataacctg acgtaattca
    17401 taacattaca ttgactgata aatataaacc aatgaaaaag aacattttta agttttcggt
    17461 actgacattg gctgttctgt ctttgacagc atgtaccctt gttcctggtc agaatttatc
    17521 gaccagtaac aaggatgtca ttgaactacc tgacaatcag tatgacctgg acaaaatggt
    17581 caatatctat cccgtcacgc caggattgat tgatcagttg cgcgccaaac cgattatgtc
    17641 gcaggctaac ccggagctgg agcagcaaat cgcgaactat gaatatcgta tcggcattgg
    17701 tgatgtgttg atggttacgg tctgggatca cccggaacta accacccccg cagggcaata
    17761 ccgtagcgcc agtgataccg gcaactgggt taacgccgat ggtgcgattt tctaccccta
    17821 tattggtcgc ctgaaagtgg ccgggaaaac acttacccag gtacgcaacg agattacagc
    17881 gcgtctggat tcagtcattg aaagcccgca agtcgatgtc agcgtcgcgg cgttccgctc
    17941 gcaaaaagcg tacgtcaccg gtgaagtcag caaatcgggt cagcagccga ttaccaatat
    18001 tcctctgacc atcatggatg cgattaacgc cgcgggcggt ctgaccgcag atgcagactg
    18061 gcgcaatgtg gtgctcaccc agaatggcgt aaagaccaaa gtgaacctct atgcgctgat
    18121 gcagcgtggc gacttgcgcc agaacaaact gctgcacccg ggagatattt tatttattcc
    18181 gcgcaacgat gatctgaaag tcttcgtgat gggtgaagtg ggtaaacaga gcacgctgaa
    18241 aatggaccgc agcggtatga ccctggcgga agcgctgggt aacgctgagg gtatgaatca
    18301 ggatgtcgct gatgcgacgg ggatttttgt tattcgtgca acgcaaaaca agcagaatgg
    18361 caaaatcgcc aatatttatc agctgaatgc gaaggacgca tcggcaatga tactcggaac
    18421 ggaattccag cttgaacctt acgacattgt ttatgtcact accgcgcctc tggctcgttg
    18481 gaaccgtgtg atttccctgc tggtaccgac gatttccggg gtgcatgacc tgacagagac
    18541 ttcacggtgg attcaaacat ggcccaacta aaatttaact caatcctggt ggtttgtacc
    18601 ggcaacattt gccgttcgcc cattggcgag cgcttgctgc gtaaacgtct gccgggcgta
    18661 aaagtcaaat cggcaggtgt tcatggtctg gtaaaacacc ctgcagatgc gacagcggca
    18721 gatgttgccg ctaatcatgg cgtatctctt gaaggacatg ccggacgtaa gctcactgct
    18781 gagatggcac gaaattacga tctgattctg gcaatggagt cggaacatat tgctcaggtt
    18841 accgcaatcg ctcccgaggt ccgcgggaag acaatgctgt ttgggcaatg gctggaacag
    18901 aaagagatcc cggatcccta tcgtaaaagt caggatgcat ttgaacatgt ctacggaatg
    18961 ttggagcgcg ccagtcagga atgggcgaag cgcctcagcc ggtaatttga gtttataaaa
    19021 tacgatgaca actaaaaata tgaatacgcc accaggcagc actcaggaaa atgagatcga
    19081 tctgcttcgt ctggtcggcg agttatggga tcaccgtaag tttattatca gcgtgaccgc
    19141 gttattcacg ctgatcgctg tcgcttactc gctgttaagc acaccaattt atcaggcaga
    19201 tactctggtc caggttgagc aaaaacaggg caacgccatt ctcagcggcc tgagtgatat
    19261 gatccctaac tcatcgcccg agtctgcacc ggagatccaa ctgctgcaat cgcgcatgat
    19321 tctcggtaaa accattgctg aactgaatct gcgcgacatg gttgagcaga agtattttcc
    19381 gattgtgggt cgcggctggg cgagattaac caaagagaaa ccaggtgagc tggcgatcag
    19441 ctggatgcat attccacaac tgaatggtca ggatcagcaa ctgacactca cggttgggga
    19501 aaacggccac tatacgctgg aaggtgaaga gttcaccgtc aatggtatgg tcggccagcg
    19561 tctggaaaaa gatggcgttg cgctgactat cgcggatatt aaggccaaac caggaacaca
    19621 gtttgtcctg agccaacgta ccgaactgga agcgattaac gcgttgcagg aaacctttac
    19681 cgttagcgaa cgcagtaaag aaagcgggat gctggaactt accatgactg gtgatgatcc
    19741 ccagttgatt actcgtattc tgaacagcat cgctaacaac tatttgcaac agaatatcgc
    19801 tcgccaggcg gcgcaggatt cacaaagcct tgaattctta cagcgccagt tgccagaagt
    19861 gcgcagcgag ctggaccagg cggaagaaaa actcaacgtt tatcgccagc agcgcgattc
    19921 ggttgacctt aacctggaag ccaaagccgt tcttgagcag attgtgaacg ttgataatca
    19981 actcaatgag ctgaccttcc gcgaggcaga gatctcccag ctgtataaga aagatcaccc
    20041 aacttatcgt gcgctgctgg aaaaacgcca gacgctggag caagaacgca aacgcctgaa
    20101 taagcgggta tcggctatgc cttccaccca acaggaagtg ttgcgtttaa gtcgtgacgt
    20161 agaagcgggc cgtgcggtat atctgcaatt acttaaccgc cagcaggagt tgagtatttc
    20221 gaaatccagt gccattggta acgtgcggat tatcgacccg gcagtcactc agccgcagcc
    20281 agtgaaaccg aaaaaagcgt tgaatgtggt gcttggtttt attcttggcc tgtttatttc
    20341 ggtgggtgcc gtgctggcgc gtgcgatgtt gcgtcgtggt gtagaagccc cggaacaact
    20401 ggaagagcac ggcatcagcg tttatgccac catcccgatg tccgagtggc tggataaacg
    20461 tacccgtctg cgtaagaaaa atttattttc taatcagcag cgccatcgta ctaaaaatat
    20521 ccccttcctg gcggtggata acccggcgga ttctgctgtg gaagccgtac gtgcgctacg
    20581 aaccagtctg catttcgcta tgatggagac tgagaataac attctgatga tcaccggtgc
    20641 gacgccagac agtggtaaaa cgtttgtcag ttcaactctg gcagcggtga tcgcccagtc
    20701 cgatcaaaaa gtgttattta ttgatgccga cttacgccgt ggttattcgc ataacctgtt
    20761 taccgtgagt aatgaacatg gcttgtcgga atatctggca ggtaaagatg agctcaacaa
    20821 agtgatccag cattttggca aaggaggctt tgatgtgatt actcgcggtc aggtgccacc
    20881 taacccatct gaactgctga tgcgcgatcg gatgcgtcaa ttactggaat gggcgaacga
    20941 ccattacgat ctggtgattg ttgatacgcc gccgatgctg gcggtgagcg atgctgcggt
    21001 cgtggggcgc tctgtcggca ccagcctgct ggttgcgcgt tttggcttga acaccgccaa
    21061 agaggtgagc ttgtcaatgc agcgtctgga acaggctggc gttaatatta aaggcgcaat
    21121 cctcaatggt gtgattaaac gcgccagcac cgcttacagt tacggctata actattacgg
    21181 ttatagttac tccgagaaag agtaaaactg aaggtaaagt gaccggatgc ggcgtgaacg
    21241 ccttttccgg tctacgcatt agacagttct tcgtttgtca tcagcctcag agcattcagg
    21301 tagctgaatg ctctttttta tacattacaa actgcacgcc ggtatgcgtg cttcattcac
    21361 gatttgcgta aaaccggcca acgaacacat gccctgcgca tttcgctctt cacatcctgc
    21421 cagggtcagt ttcacctctc cgggcggcgt atttaatgac agcggagttt tatcacgcat
    21481 ctgctgtaaa gtctggaaga ccagcgaaac ctgaatccac tggctgttat cgcttagccg
    21541 acgccagcgt tcaaacacca gttcaccacc tggcggcgtg ttatccggct gacctggaag
    21601 cgtccagttg agctccagtg cgccgccgag atttgccaga ttagtatcgt gtccggcgat
    21661 aaacagcact gaagtgggta atgtcacacc atacgcctgt ttttgcggtg gatggggagt
    21721 caacgctatc atgatcaaat ccaacaacgg ggtggcgcgg ctgcgggcaa cctctggcgt
    21781 gcgttgtagt aaataaaatt gcgcgttatg caaacttagc aacgtgttcc actggtgtga
    21841 atcagtgatc cttccccacc ccggctccgg cattccctgt gcctgttgca ggagaaatat
    21901 ttccgtcagc attgatgcga ggcttaccgc accggttaat gagacattgt cggcgctcac
    21961 cttgagttcc gatggtaatg cctgcgttaa tgaacagctt tcgtcctgtt tctcacggtt
    22021 aaggcacaag tttgattgcg gaaaattaag cacccgttcc agttcgcgaa acgccgtttg
    22081 ccgatgcccg gtaaagtcag caattgaccc tcctgccctg ctgaggatcg cgtcagtcac
    22141 gttcgcgtta tccagttggc aaacgccagt ttttagagga ttaaataacg gatcgggact
    22201 ggacgtatct gcctgggtat gtacggttat tgcacagtca ggtgccagcc cggcggcgaa
    22261 ggcttcgcct gttttacggg tacgctcgtc gacatcagca ataatcgcga cctgaccagg
    22321 ctgcgggcag ccctttttcg tcagcaatcc gtcggctacc agacgctggc gttggtaatg
    22381 tccgagataa gcgattagct caccaccgcg cggtgtcagc caacccagtt ttaccggcca
    22441 gtttggccat gcgtctgggg tgacatcctg catcagttgc gtggccttgg ttggggcacg
    22501 cacaccatga cgactgacaa tcaccacact ttccagcttc agctccggct ccggctcact
    22561 ctgagcgaat gcagattgcg gggttaacgg aatcagaaga gataaaaatg ggatcaagat
    22621 cgctttcatc gatatgtttc cgcttttcat atccgacatt attgattgcc tgatacgacg
    22681 ctaatgcgcc gcatcagact tacgttcgtt aacttttcaa tcgcgggtcc agccatacca
    22741 acacaagggt ggagagcgaa cacatcaaca aaatgccgac gaaccaaagt aaataccaca
    22801 ttgttcctgc tccttagtac agctcgtttt cgttacggcg gagagtttct gtcgtcatgc
    22861 gcccccacat tttgtagtag ctccagagag tgtagagcaa cacaatgggc aaaaatatca
    22921 gcacgattac caacataata ctcagcgtca gctgactgga agtgctgtcc cacaacgtca
    22981 ggctggagat tggactcacg cttgacggca tgacaaaggg gaacagcgtg atgcctgccg
    23041 tgaaaatcac gccaaattgc atcaatgacg ccatcaaaaa tccccaaccc gggcggccac
    23101 gataaatcgc catcaccgtc agcaatgggc agaagaaccc cagcagcggg aagatccaca
    23161 aaacgggcga ctcgacaaaa ttattcatcc aggcaccagg tagcactgcc accagtttca
    23221 ttaacggatt ggaaggaccg ttagcatcct gggcgagcag tacaaagcca tcaataccga
    23281 cccacagcca gtaacccgcc agcaaaaagc acagcatcac caacagtgca gcgcgtttag
    23341 tcgacagctg tgaacgcaga tgaatcacac caacagtttt cagttgtaac cagacgccac
    23401 cttgcaaaat caccatccca aggctgagca atccgcacaa taaagggaat ggcgtcagca
    23461 gttgccagaa gctgccgaga tactccacgc gtaattgcgg agtgaaggca aacggcacgc
    23521 cgagcaacaa gttgccgaag gcgataccga agactaccgg cggcaccaga ctgccgatga
    23581 ccagaccggc atcccacatt ttacgccagc gtgcatcggc gatttttccg cgataatcaa
    23641 aggccagcgg gcggaagaac agcgagcaca gcaccaggat catcgccaca taaaagccgg
    23701 aaaacgccgc tgcatacact ctgggccagg cggcaaataa tgccccacct gcgaggatca
    23761 accagacctg attgccttcc cagtgcgcac caacgctatt aatcactatc cggcgttcat
    23821 catcatcacg cgccaccagc ggcagcaaac agccaatccc catgtcaaat ccgtcggaga
    23881 tcataaagac caccaggatc acgccaatca gcagccacca gatgaagcgc aatgtttcat
    23941 aatcaaacat gattttctcc tttacccctg ttgctgcgtc ggttgttcac tctgcatcgc
    24001 gctcggcccc agacgggcat atttctgcat caggtagact tcggcgatta agaacagggt
    24061 gtaaagccct acgatcatga tcagtgagaa agccagttgt cctgtggtta aagcggagtg
    24121 cgcggagtat gtcggtaaga tgtcctgtat cgcccacggc tgacgaccaa actcggtcat
    24181 aaaccacccg gcttcaatcg caatccacgg caacggcaaa ctccagagcg ccattttcag
    24241 cacccagcga tgctggtcga ttttgccacg cagcgtctgg acaagcgcaa tcagcatcac
    24301 cagtagcagc agggaaccac agcccaccat gatgcggaaa ctccagaata ccggcgcaac
    24361 ctgaggtatc gcgccacgca tcgccgcctg gtactgtgcg gctgtgacat gattcatatc
    24421 cggcgcatag cgggagagca acatgccgta gcccaggtcg ccttccagct cccggaatgc
    24481 ctgcaacaca tgcggctcac gattgccttg cgatatttcc tgcattaaca gccaggccat
    24541 acgtccgcgt tgcaagcgtg ggtaggtttc agccatcaaa ttcttcagac ccggcacggg
    24601 tttatctaat gagtgagtgg cgaggatccc taacagcacg ggaattttga tggcaaaggc
    24661 gttacgctct tgatcctgtt ccggccaggc aaccacatgg aacggtgcag gagcaggttc
    24721 cgtttgccac tccccttcca tcgccgccag ttttaccggt tgtacttgcg cgacttcata
    24781 cgcagaactg tctccgagtt gcagggtacc gataatcgcc agagtaccga agacggaacc
    24841 gatggcaaac gagcgtaatg cgacatcgcg ctcccgtccg cgcagtaaat accaggcgct
    24901 gatcgccata ataaacatgg ccccggtcac gtagcccgcc attacggtgt gcacaaattt
    24961 cacctggctg accgggttaa agaccagctc gctgaagctg gtcatctcca tacgcagggt
    25021 gtcgatatca aaatgcgcac cggtcgggta ttgcatccaa ccgttggcat tcaatatcca
    25081 caatgcagag agatttgaac cgaacgccac cagccacgtc accagcaggt gctggtattt
    25141 attcagccgc tgccagccga agaagaaaag cccgacaaag gtggattcga ggaagaaggc
    25201 cattaatgct tccatcgcca gcggtgcgcc aaaaatatcg cccacatagt tggaatagaa
    25261 tgaccagtta gtaccaaact gaaactccat ggtcaggccg gtagccacgc caagagcaaa
    25321 gttgataccg aagagcttac cccagaagcg cgtcatatcg cggtagattg ttttgccggt
    25381 gaccacgtaa atggtttcca tgatagccag caaaaaaatc agccccaggg taaggggtac
    25441 aaataaaaag tgatacagcg cggtcagagc aaactgccag cgcgataaat caatgacatc
    25501 ccacatgcgc actcctgtag gcgaagagag aaaaataacc cgttaatggt agatctccct
    25561 ctttatcctg aaactctacg accagaggta ttcgccgatt gcgatattca taagcctgaa
    25621 agcgatactt accgtctttt tacgtcggtg cagcttccgc cagccactga catacctcac
    25681 taagccgctg cgcagagtcg accaaatctt ctggcgctgc cagcaccact tccggtattg
    25741 ggcagatttc ataactttcc agtaacgggc cttttaaggt gtccgtacag cgtaaatgcc
    25801 agacatggcg taaccccgtg gcgttgatat agctctcgcc atagccaatg gtacgaatct
    25861 gaatatttcc cggcccacag agacgtgaga gaaaaagccg atcagcctcg ctgatgggta
    25921 attgcgtcag attaatgctg tgcggctgcg cgtcggggtt acgtacatgc gccagtaact
    25981 catgcgccaa cggtaggcca ttcatcaggc catcaatggg cggcggtaac agcgaatctg
    26041 tcggcaagag attttgcgtt gccgcctgcc acaacgccag cggcgcgcag ccagcctcca
    26101 gtttgtcctc cagcaacttt tcgccgcgac gtctgcgcac ccgccataat ccgcagaaga
    26161 tcgcctcctg tatttcactt tcgctgtcgt cagcctgctg aatgcgcact gacacttcac
    26221 cttccccgag aagcgtattg ataaagtgac gatcgtcggc gttgagattt aacagatcga
    26281 ctgccagcac ctcgcccaac ggcggctggc gttgacttag ttgatgttgt aactcgttta
    26341 acagcgcaat cacctgcggg ctgtgagcac attgctccag ggccgccata ctcggttcat
    26401 cattcacctg acaggtgatc ggcagtggat tcatgctgaa actgtcatcg ttcggttgcg
    26461 ttcttggccc cagcagatgg aaaaaagttt cgctcatgag gcacgctcct gctgtggttc
    26521 gacaagccca cgcatcaggt ttatcagttc cgcccacggg tgaataccat tcagcacgcc
    26581 gcgatagttt ccgccggtaa acactaatgt ggcgggaaag cgaaagacgc caaagcgatc
    26641 tccgatggct tcgctctgtt caaggtcagc aatcgccacc tgccatgtgt agtcgggaaa
    26701 ctcgcgcagt aattcgccaa tcattaccgg attatcgctg acctctggcg tgcgtttcgg
    26761 gtcactgctt aataacacca cgccgtctgg cgcttgcgta agccagtcgt caagacggaa
    26821 ttcactaacc ggcgtccagc cgcgcgccag cattcgttgc cacaacgcat caaatggcgt
    26881 gtcgttgctc atccctgctc ctccagtgtc atcctgtact ggcgcaagcg aacgccttcg
    26941 taattttcca tcgacagaca gtcataattg agacagcgcg attcattagc cggttgcggc
    27001 acaattcccc atgcagccag ctgcgccagc gccgcctgtt ccgctgcggg cagttgctcc
    27061 cgtgccagtt cgctcaggct gccgccgtag tcgtcgagca ttgcgggttg cagaccgacg
    27121 agggcaatat gtgctggcag atgtccgcgg atatctgcca gcgccagcac ttcggagaaa
    27181 ctgttctggt gcaggctcat tttcttcgcg ctgagataag ccggaatacg ttctccggca
    27241 taggttcgca gcgttcctgg ttccagcccg tagtcaatgg catcgagaat caacagatgg
    27301 ctggcgcttt cgacataccc cagcaagttc agtccctgag taccgccatc gacaatctcc
    27361 acagactcgg gccagtggta atgggcatac aaccgttccg ccacccgcac gccgaaacct
    27421 tcatcggccc acagcaggtt gcccagcccc attaccacca cgcgttgctc gctcatgaac
    27481 gctccttgtt acttattttg ccaaatttgt ggctacggta gccgttgacc atggtggaga
    27541 tcaccgtgtc gtcggacatg atgtcttcac gcagcgccat atagacatga ccgatcacaa
    27601 acgcgccaat cagccacatc cccagccgat gccagctgtg aatgtccatt gagttgcctc
    27661 ccgtccagta gaaaaattcc accacataac ggaacggcgc aaaaatagcg tactggctgt
    27721 gttcgctgta cagcgcaaag ccggtgatga tcataaagac cgacatcagg aaatagccaa
    27781 acatcgccgc ctgggcgatg gggttatggc ctatatcggc actcggacgt tttgccagaa
    27841 acagatacca gcggatttca taccacacgc cctgccacca gcttttacgc catactggca
    27901 cgataaacag ctcgcgggag tatcgattgc caacaaaagc ccagtagatc cgcatcagca
    27961 agaccacggt aaaaaccatc ccggcgctga agtgaattaa cctgatgtag cccatataga
    28021 acagatacgt cgcctcgccg ctgacggaag gtagcggttt gccgataaag taaccggtga
    28081 ccatcaacac cgccatgcat aacaccgtca gccagtgcca gatacgcact ggcgcttcaa
    28141 agacatagtg gctgacaacg ttgtcgcttt tctgttgcat gatgattctc cttcgctgtt
    28201 aacgcacctg cacggagatc agctcgctac cgtcgtcgcc cagcacgtgt gttgaacagg
    28261 cgaggcacgg atcaaagctg tgcagagtac gcaggatctc cagcggttgc tcggggatcg
    28321 ccattttggt gttcatcagc gccgcttcat aagcgccaat ctgccctttg ggatcgcgcg
    28381 ggctggcgtt ccaggtggtc ggcaccacgc actggtagag atcaatcttg ccatcgcgaa
    28441 tggcggccca gtggcctaac gccccgcgcg gcgcttcggt aaaaccgaca ccacggcact
    28501 ctgtcggcca ggttgtgggt tcccattttt ccgtggaggc ggtggcaaga ttgccgtttt
    28561 tcaggttagt catcagcttg ttgaagaaat actgcaactt acctgcggcc cactgtgctt
    28621 cgtgcgcgcg gcacaaaatg cggcccaacg ttgactggat accggaaagc ggcaggttca
    28681 gcgccgacat catgcgatcg accgactcaa cggtcgcagc atcgcctttg tgataagcga
    28741 ttaacgtgcg tgccagcggc cccacttcca tcgcgttacc gcgccagcgc ggcgctttga
    28801 tccacgagta gcgttcctgt tcattcagct gctgaatgtt ggtatcgctg cctttgacat
    28861 cgccggggtt gtaccacggg tcggtgatgc catcgaacgg atgacgcccg acctgatcgt
    28921 tgggataacg ataccaggcg tgatcgacaa actcctgcac ctgctgcgga tcaaccaaat
    28981 ccactggcag cacattgttg aagtcgccgt taatcaccgc gccgccaggc atcagcagac
    29041 ttttctcacc aaagtcgttg gcaatatccg ggaatgcgcc gtagctgagg acacatttat
    29101 cagaaagacc cgtgccgatt tcgctccacg gtttgttgaa ctgaccgatg gctaaggcgt
    29161 cggggatcat cacgttgtta atgaagtctg ccgtgcgggt gataatcgac tgcaccaggt
    29221 tcaggcgttc catattgacc gccccgaccg cgccgctttc gtcaatgtta atggcgcaag
    29281 gcatcccgcc gacaatccag tttggatgcg ggtttttacc gccaaagacc gcgtggattt
    29341 tgacaatttc acgctggaaa tcgagagctt cgagatagtg ggcaaagccc atcaggttgg
    29401 cttctggcgg caacttgtac tgcgggtgcc cccagtagcc attgcggaag atccccaact
    29461 gcccgccttc aacgaatttc ttcaggcggt tttgtacgtc gaagaaatag ccaggggatg
    29521 atttcggcca agaggagaga ctttgcgcca gttcggaggt tttccgcggg tcggctttca
    29581 gcgcatccaa cacatcgatc cagtccatcc cggcaagctg atagaagtgc accagatgat
    29641 cgtggcacca gagcgttgcc agcataatgt tgcggatgat attggcgttg tccggcactt
    29701 taataccgat ggcatcttcg atggcgtaaa ccgaagccag agcgtgtacg ccagtacaga
    29761 cgccgcagat acgttcaacg aacgcccacg catcgcgcgg gtcgcgccct tgcaggatga
    29821 tctccagccc gcgaaacatg gtgccgcagg agacggcatt ggtgattaca ttctgatcgt
    29881 taatattcac ttcgcagcgc atgtggcctt caatgcgcgt aatcgggtcg accaccaggc
    29941 ggcgtccggc attattgatg gtgtatccct gagtttcgta ctgagtgctc atgcctgttt
    30001 atcctcattg cctggctgat gttcggtttc tgtaggttgc tgattatgac gtctgcgctg
    30061 gtcaacggcg ctggcgactg cgtgcacacc aacagccgct gccaccacac caagcgcggt
    30121 taaaccgacg gtatcggcgg tggaatgagt acccatttgt ggaatatcga ccacgcggct
    30181 gtagaacgaa ccgcgatccc agaaaccatt ttccgcacag cccaggcagc cgtgaccaga
    30241 ctggattggg aaagaaacgc catcattcca gcgtgtggag gaacaggcgt tataggtggt
    30301 aggccctttg cagcccattt tgtacaggca ataacctttg cgggcagcgt catcatccca
    30361 actctggacg aactctccgg cgtcgaagtg agcgcggcga tagcatttat cgtggatacg
    30421 ctgaccataa aacatcagtg gacgacccat tctgtcgaca tctggcaagc gatcaaaggt
    30481 caccatgtaa gtaatgatgg cgctcatcac atccgggatc ggcgggcagc caggtacttt
    30541 gataatgggt ttgtcggtga tgactttgtc gatagacgtt gcctgtgtcg gattaggtcg
    30601 cgcggcctgc acgcagcccc aggacgcaca ggttccccag gcgataatcg cgctggctcc
    30661 ggcagcggca cgtttgagtt tctcaataaa cggtcgaccg ctgctgatac agaacatccc
    30721 ctgctcgccc agcggcggat taccttctac tgcgaggata tatttgccat tgtattgcgt
    30781 gatgatgtct tcaaagactt cttccgcctg ggttccggcg gcagccatca aagtatcgtc
    30841 gtaatcgagg gaaatcaggg aaaggatgac gtcctttgcc agcgggtgag cggagcggat
    30901 aaaagattcg gtacagcagg tgcattccag accgtggatc cataccaccg gaatgcgcgg
    30961 tttgttctcc agcgcccagg caatctttgg tgccattccc gcgcctaatc ccagcgacgt
    31021 ggcagccaga ctacaatatt tgagaaagct gcgccgggta acgccctgac gccgcatggc
    31081 ctggtaaaat gtttcctcgt tattcatatc gcacgtctct tctccttgcg acaccggcag
    31141 gacaccgggc gcttatggtc ggcaagcgac gtccctgtag caaggcaaac catcgcttca
    31201 cgtgagatgc tgaaaacgaa agctcatcct tctgcactgg cgcacgtcgc cagaaagtat
    31261 tgttaataaa gcgtagtgaa acttttgcac aaaacaatac aaactgtgtg gatttatctt
    31321 ttagcgataa aaatggacct atttttcttt ttgccgggcg gagggatgtt tagccagttg
    31381 ctaaacgagt aaaagagaag gaatttatgg tgatgaaatg atcaatctgc ttttatttca
    31441 cgctatcaat gcacttttgg ctgtttttca ggcaaacaaa cagattaggg gtttacacgc
    31501 cgcatcggga tgtttatagt gcgcgtcatt ccggaagtgt ggccgagcgg ttgaaggcac
    31561 cggtcttgaa aaccggcgac ccgaaagggt tccagagttc gaatctctgc gcttccgcca
    31621 aataatcaag gggttaccaa atggtagccc ctttgctttt ttggctcttg gtataaactt
    31681 ggtataccaa ttggtatatt tttattcgga tgagattatc ggtcatgagc ggaacctcag
    31741 gttcctcgtc tgatgccgct cttgcaacac gttacgctgc cgagtatttt tgtaaaacat
    31801 ggactgctcc aggcctgaac caggcagaag gatacaaagc catctcagat ctgtcgcatc
    31861 actattttcg tgcagaaggc tctagccctc cccaatcttt tttgtgacac cctgtttcac
    31921 tgaacatgaa gacactcacc ttgtccctga tattcattag catcatcctg attatttaac
    31981 ttctctattt agggaagccc gtctgataca aagcttctat tcctcgttaa atattctcca
    32041 ttctggcgcc aagccctatc tatagctgat tgaaaacaac tctaaaaacg taataatatc
    32101 aaatagttta ttcctacagc cgatcatttt gagtaaaata ttgacgagaa tttcacaaaa
    32161 ggtagatttc atgtaaacgg ataaataaga ctatccaa
//
LOCUS       DAZKFZ010000033        32194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000033 DAZKFZ010000000
VERSION     DAZKFZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            132..413
                     /locus_tag="ACHOQW_001428"
     CDS             132..413
                     /locus_tag="ACHOQW_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572523.1"
                     /translation="MTETELLRLIRRVAGINQQADEQATQPDSVTAENYVRVVAEVMH
                     RDGIQLNDADMRDIRIRVLEMLAYRRRIQTYREKAKITYQWKKPERLRR"
     gene            complement(537..836)
                     /locus_tag="ACHOQW_001429"
     CDS             complement(537..836)
                     /locus_tag="ACHOQW_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572524.1"
                     /translation="MRMTRHKKQILELYKPEYRDWVRVEAGDPPFDVRGVTVLLYGSE
                     YQRYHIEATRRTLNAMVNDGILYRVKVREPRFDVRIGSDGAHCTVIRYGLGKSDD"
     gene            949..1251
                     /locus_tag="ACHOQW_001430"
     CDS             949..1251
                     /locus_tag="ACHOQW_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9572525.1"
                     /translation="MVHDDKAEELEAKGLWRRAANRWGELLKQAASDEARQYVTERRT
                     ACIRKATITREPERDLICAIRTAATKTLTDMGIDLKKEDPLRGIQHGHTKRDKRRK"
     gene            1977..2297
                     /gene="clpS"
                     /locus_tag="ACHOQW_001431"
     CDS             1977..2297
                     /gene="clpS"
                     /locus_tag="ACHOQW_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP9572526.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            2328..4604
                     /gene="clpA"
                     /locus_tag="ACHOQW_001432"
     CDS             2328..4604
                     /gene="clpA"
                     /locus_tag="ACHOQW_001432"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP9572527.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(4950..5037)
                     /locus_tag="ACHOQW_001433"
     tRNA            complement(4950..5037)
                     /locus_tag="ACHOQW_001433"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5001..5003),aa:Ser,seq:gga)
     gene            complement(5291..5509)
                     /gene="infA"
                     /locus_tag="ACHOQW_001434"
     CDS             complement(5291..5509)
                     /gene="infA"
                     /locus_tag="ACHOQW_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9572528.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(5794..6498)
                     /gene="aat"
                     /locus_tag="ACHOQW_001435"
     CDS             complement(5794..6498)
                     /gene="aat"
                     /locus_tag="ACHOQW_001435"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP9572529.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(6540..8261)
                     /gene="cydC"
                     /locus_tag="ACHOQW_001436"
     CDS             complement(6540..8261)
                     /gene="cydC"
                     /locus_tag="ACHOQW_001436"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP9572530.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(8262..10028)
                     /gene="cydD"
                     /locus_tag="ACHOQW_001437"
     CDS             complement(8262..10028)
                     /gene="cydD"
                     /locus_tag="ACHOQW_001437"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP9572531.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(10151..11116)
                     /gene="trxB"
                     /locus_tag="ACHOQW_001438"
     CDS             complement(10151..11116)
                     /gene="trxB"
                     /locus_tag="ACHOQW_001438"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9572532.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            11661..12155
                     /gene="lrp"
                     /locus_tag="ACHOQW_001439"
     CDS             11661..12155
                     /gene="lrp"
                     /locus_tag="ACHOQW_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP9572533.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            12290..16318
                     /gene="ftsK"
                     /locus_tag="ACHOQW_001440"
     CDS             12290..16318
                     /gene="ftsK"
                     /locus_tag="ACHOQW_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9572534.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            16473..17084
                     /gene="lolA"
                     /locus_tag="ACHOQW_001441"
     CDS             16473..17084
                     /gene="lolA"
                     /locus_tag="ACHOQW_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP9572535.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            17095..18438
                     /gene="rarA"
                     /locus_tag="ACHOQW_001442"
                     /gene_synonym="mgsA"
     CDS             17095..18438
                     /gene="rarA"
                     /locus_tag="ACHOQW_001442"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP9572536.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            18529..19821
                     /gene="serS"
                     /locus_tag="ACHOQW_001443"
     CDS             18529..19821
                     /gene="serS"
                     /locus_tag="ACHOQW_001443"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9572537.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            20060..22504
                     /gene="dmsA"
                     /locus_tag="ACHOQW_001444"
     CDS             20060..22504
                     /gene="dmsA"
                     /locus_tag="ACHOQW_001444"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP9572538.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            22515..23132
                     /gene="dmsB"
                     /locus_tag="ACHOQW_001445"
     CDS             22515..23132
                     /gene="dmsB"
                     /locus_tag="ACHOQW_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP9572539.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            23134..23997
                     /gene="dmsC"
                     /locus_tag="ACHOQW_001446"
     CDS             23134..23997
                     /gene="dmsC"
                     /locus_tag="ACHOQW_001446"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP9572540.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(24033..24659)
                     /gene="ycaC"
                     /locus_tag="ACHOQW_001447"
     CDS             complement(24033..24659)
                     /gene="ycaC"
                     /locus_tag="ACHOQW_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP9572541.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            24974..26122
                     /gene="ycaD"
                     /locus_tag="ACHOQW_001448"
     CDS             24974..26122
                     /gene="ycaD"
                     /locus_tag="ACHOQW_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572542.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            26332..27762
                     /gene="ycaM"
                     /locus_tag="ACHOQW_001449"
     CDS             26332..27762
                     /gene="ycaM"
                     /locus_tag="ACHOQW_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9572543.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(27763..27873)
                     /locus_tag="ACHOQW_001450"
                     /pseudo
     CDS             complement(27763..27873)
                     /locus_tag="ACHOQW_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(27972..28712)
                     /gene="pflA"
                     /locus_tag="ACHOQW_001451"
     CDS             complement(27972..28712)
                     /gene="pflA"
                     /locus_tag="ACHOQW_001451"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP9572544.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(28904..31186)
                     /gene="pflB"
                     /locus_tag="ACHOQW_001452"
     CDS             complement(28904..31186)
                     /gene="pflB"
                     /locus_tag="ACHOQW_001452"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9572545.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(31241..32098)
                     /gene="focA"
                     /locus_tag="ACHOQW_001453"
     CDS             complement(31241..32098)
                     /gene="focA"
                     /locus_tag="ACHOQW_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP9572546.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         8043 a         7847 c         8491 g         7813 t
ORIGIN      
        1 ctgttgtttt taatgtaatc gatgcaaaaa agatgatgga gaaggttgtt ttttgttcga
       61 catcttttag cgtgacagat tctttacaaa aaatctgagc ttgttttctt aaccagcgcg
      121 atgggggcac aatgacagaa accgaactac tgcgattaat ccgccgcgtc gccggaatca
      181 accagcaggc tgacgaacag gccacgcagc cggacagcgt gaccgcagaa aattatgttc
      241 gtgtggttgc tgaggtgatg caccgtgatg gtatccagct taatgatgcg gatatgcgcg
      301 acatacggat ccgcgttctt gaaatgctgg cctaccgtcg ccggatacag acgtacaggg
      361 aaaaagccaa aataacgtac cagtggaaga agccggagcg gttacggcgg taacttgctg
      421 aagttaaagc ataatgcaaa tttgcactct ggcctaaccc gttaatattt cagcaaagtg
      481 caaatttgcg ttctgtagct ggtggctggc agaatgtaaa tttacattct ggtgatttaa
      541 tcatctgatt tacccagccc gtaccggatt accgtacagt gagctccatc gctaccgata
      601 cgcacatcaa agcggggttc gcgcactttc acgcggtaca ggatcccatc atttaccata
      661 gcgtttaacg ttcgtcgggt tgcctctatg tgatatcgct gatattcgga accatagagc
      721 agcactgtca caccacgaac atcaaaaggc ggatctccgg cctccacccg cacccaatcg
      781 cgatactcag gtttgtaaag ttcaagaatc tgttttttgt gcctggtcat tctcatgtgt
      841 gattccttat tgtgggacag tactgtcata caataagcta tgagggaaat tatcaaaaga
      901 acggaaacca accattcaga tttaacaggt atcaatggga ttatgcttat ggtgcatgat
      961 gacaaagcgg aagaactgga agcgaaaggt ttatggcgac gggcggctaa tcgctggggt
     1021 gagttactga aacaggcggc aagtgatgaa gcgcgtcagt atgtaacgga acgacgaacg
     1081 gcatgtatcc ggaaggcaac cataacccgg gaaccggaaa gagacctgat ttgtgccata
     1141 agaaccgccg ccacaaaaac actaacggac atggggatcg acctgaaaaa agaagatcca
     1201 ctgcggggca ttcagcacgg acatacaaag cgggataaac gcaggaagta atttttttcg
     1261 tgtaaccagg caaccgacta tctgaaagaa gtcagaacta cgaaattttc gtagtttaat
     1321 ttgcagccag gctatcggag atcgtgacgg agagtgacaa ccagtgacac gacgtgacaa
     1381 aaagacggtt taaccgttaa ttaaaaatcg atgggatttt gtgcttttcg gacgagtggc
     1441 caacgtcatt tttagcgaaa agctctgatt aaggtcattg gctgaatggt cataatgacc
     1501 acgcagatca tcaaggcaac cataacgact tccgttacgg ttgatgcttt taccccattg
     1561 ggggaaaggt attacgataa tcataacacc taccgaacag gtaaagccca ccagcctgat
     1621 taacagttaa ccggaaaaaa atccggtatg gggggagtgg gtaggtgggg atccccatat
     1681 cggggaacat ccaccagctt cataacggat ggccggaaaa tgataacggt taaagcgtgt
     1741 tgatctttgt tcgtggttgt tcgatattgt tcgtgaaacg gtgtagttaa tggtgtagtt
     1801 aatttcgctg tttttgacag agttgttatt tgtaacacag tgaaaaataa agactaatca
     1861 atgaaatgtt ataaatgata gttatctatc atgtggagta gattggtcag gcaaataagc
     1921 tcttgtcagc ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg
     1981 gtaaaacgaa cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc
     2041 taaaaccgcc atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt
     2101 ttgttattga cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga
     2161 tgctcgctgt tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag
     2221 aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta
     2281 cgctagaaaa agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag
     2341 aactggaact cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta
     2401 tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg
     2461 aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa
     2521 ccacacccgt tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc
     2581 agcgtgtact gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg
     2641 gtgcaaacgt tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc
     2701 gtaaacatga agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag
     2761 acgagccgac acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg
     2821 gggaggaacg tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa
     2881 tcgacccact gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc
     2941 gccgtaaaaa caacccgctg ctagtggggg aatctggtgt cggtaaaacc gcgattgcag
     3001 aaggtcttgc ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga
     3061 tttactctct cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa
     3121 aacgttttaa agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg
     3181 atgagatcca caccattatc ggtgcgggtg cagcgtctgg tggtcaggtt gatgcggcta
     3241 acctaatcaa accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc
     3301 aggagttcag caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg
     3361 atattactga accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt
     3421 atgaagcaca ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg
     3481 cggtgaaata cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag
     3541 cgggcgctcg cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg
     3601 atattgagtc cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg
     3661 accgcgatac cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata
     3721 aagccattga ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac
     3781 ataaaccggt cggttcgttc ctgtttgccg gccctaccgg ggtcgggaaa acagaggtga
     3841 cggtacagct ttcgaaagct ttgggcattg agcttctgcg ctttgatatg tccgagtata
     3901 tggaacgcca taccgtcagc cgtcttattg gtgcgcctcc gggatacgtt ggttttgatc
     3961 agggcggttt gctgactgat gcggtcatca agcatccaca tgcggtgctg ctgctggacg
     4021 aaatcgagaa agcgcacccg gacgtgttca atattctgtt gcaggtgatg gacaacggta
     4081 cgctgaccga taacaacgga cgcaaagcgg acttccgtaa cgtggtactg gtgatgacca
     4141 ctaacgccgg ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca
     4201 gcaccgatgc gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg
     4261 acaacattat ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat
     4321 tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg
     4381 aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg
     4441 cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc
     4501 tggtggacgg cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg
     4561 gattccagag tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag
     4621 gttggtgaag tccgtaatct cgaaagaggt tacggacttt ttatttatgg ggtggaacgg
     4681 tgaaaaccct atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg
     4741 taaggataaa aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacgggc
     4801 taaacgtgta gtatttgagt tcactgccgt acaggcagct tagaaattca ctggtaacat
     4861 actccacccg cccaccatgt tcactgccgt acaggcagat aaaatgcgaa aaaaaagccc
     4921 gtactttcgt acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat
     4981 acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa
     5041 ttgttttgct gccaaacctc atgggtggca acggggcgct actataggga gttggagtaa
     5101 aacggtcaag aagaatttta atgataatta ttgtttgctc atactgtaaa caacttgtgc
     5161 agtatatcta catcgagaca agttatggac ttatacttcc aaagtacttc atgcatatca
     5221 caaaataaaa aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc
     5281 ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca
     5341 gttcaacagt cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg
     5401 agatgtgtgc agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca
     5461 acgtttcaag aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg
     5521 gggtatcact accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa
     5581 gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta
     5641 agccgcacca aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga
     5701 cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa ccaactctgc gaccgcaatt
     5761 ataacactct ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa acaagcatcg
     5821 tggtacccag aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattaaggta
     5881 attccggcgg ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca
     5941 gtcgataagc ttaccgccat gaccgataaa ttcctcacag aataccagaa gcgccgtttt
     6001 agacgcattt tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac
     6061 gccgtacata ccgccgacaa gctcatcttc acgccagact tcaatggagt gggcatgacc
     6121 gagttcgtga aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc
     6181 gcgatcgctg gcacagcctt caatgacctg accaaaagcg taattcatcg tgacacgata
     6241 gggcgagcgt ttatgaaatc gcttcatact acggctgata tgcagtgatt ctggccatag
     6301 caccgcgcgg ggatcgggcg accaccagag gatggggtcg cctggagaaa accacggaaa
     6361 aataccacgc tggtaagcca ttaacaggcg cgcagggcta agatcgcccc caagtgccag
     6421 caggccgtta ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga
     6481 aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct
     6541 tacaaaccct gcttgaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta
     6601 ccttgctcaa taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg
     6661 cgaagtcgat gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca
     6721 aggatctggc tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt
     6781 ggcgcatcat gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc
     6841 tggcgtccgc cttcacctaa ccaactgttg agacctgcat cctcgagctg cttctccaga
     6901 ccaacgcgac gtaagatcac cgccagagcc tcatcactac tgccaggcga ggcgagtaac
     6961 agattatcac gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc
     7021 tgtcgtagag ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc
     7081 tgttgcgggt cccatgcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt
     7141 cgcccgagaa tcgctatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc
     7201 tgttgggatt gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg
     7261 gcaacacgag tttgggtatc agggaaggtg acctccggtt tttgatccgt taagtcagag
     7321 atacgtacgg cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc
     7381 aatgcttcaa acgcggctaa cgcgcaaaag acaaacaggg caattaacgc gccgggttga
     7441 gcattgccgc caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca
     7501 atgagcagca ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc
     7561 agccattgaa tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg
     7621 gtcagctcag cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga
     7681 tgagtcagat tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa
     7741 agcgtcagta acataatgcc gcccagcgta aaggcgaggg tggaatcaag gaaacttaac
     7801 ccgattgtca ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg
     7861 taaagatgat cgagcgtatc aacatccgcc accacgcggt tgagcaattc gccctgacga
     7921 tagcgcgcca gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga
     7981 tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca
     8041 gtacgggtga ttgctgcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac
     8101 agtccggcaa cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga
     8161 ccgatactgg cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta
     8221 tgacgtttat acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga
     8281 tgggccagta atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc
     8341 tcaataatcc ggccatcctg cataacccaa atgacatccc agtcagcaag atcttctaac
     8401 tggtgggtga ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg
     8461 cgctgttcac tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag
     8521 ggatttagta acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca
     8581 gcctggtcgc caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc
     8641 caggcgttat ccagcgctgc ttgtaattct tgttcgctgg catcaggtcg cgccagtagt
     8701 acgttatccc gtaatgttgc tgccggtaat tgtgggtttt gaccaaccca ggagagatgt
     8761 ttacgccatg attctggtga taaatcgcgt aattctatcc cgttgattcg taatgatccc
     8821 tgatatgaga gaaaaccaga aagcgcgttc agcagtgagc ttttacctga accgctgcga
     8881 ccaaccaaca ctgcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt
     8941 ttaccttccg gcgacgtgat aaacagatcc tcggcctcaa tggtcagcgg atcggttgat
     9001 gctaattccg tctcaccgcg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga
     9061 ctgtcagctg caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat
     9121 ggctggaaaa actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca
     9181 gtatcgtagt gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc
     9241 agagcaattg acagcgaggt aaaaaattcg agaatgccgg aggataaaaa cgccagccgt
     9301 agcacttcca ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca
     9361 gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc
     9421 ccacttaagc gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccatccca
     9481 accagcgcca taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc
     9541 cagttagagg ggaagatcgc caccacaatc agcaacggca ccgacactgc cagcgccatt
     9601 tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc
     9661 gcccagctcc ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg
     9721 agaacctgac ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg
     9781 cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc
     9841 agcagggctt cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac
     9901 catgcctggg caatgatcaa tatgccgctc acaaagccca gcagacgaga aatattcagc
     9961 caacgttggg agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat
    10021 ttattcattg cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac
    10081 ggggaaaaaa taaaggcgac ccatagtcgc atggtgtcgc cttctttact tttgttactg
    10141 atttgtaaaa ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg
    10201 ccatgcagcc tgtaccagca gatgtaatgg cctggcgata aatgtgatcc atcacgtcgc
    10261 ctgcggcaaa gacgccagga atgctggtct gggtggcatt accatgaata cccgactgca
    10321 ctttgatgta gccgttttcc agttccagct gcccttcgaa aatggcagta ttcgggctgt
    10381 gaccgatagc aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg
    10441 tatcgcgcag acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac
    10501 ggttggtgtg cagaatgatg ttgccgttct ccactttatc catcagacgc ttaatgagga
    10561 ttttttccgc gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag
    10621 acagatacag cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt
    10681 tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac tccacggcct ttaaaggctt
    10741 cttcagaggg caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc
    10801 aagtgtattc gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt
    10861 tgatatgatc aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca
    10921 ttaataacgg accggtcaga tcgtttggat cgccaggcca gttttccact tccgtggtgg
    10981 tggtcagttg gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg
    11041 ccgcatagac agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt
    11101 gtttggtcgt gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg
    11161 aatttacaga cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc
    11221 atcgataagc aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt
    11281 tataagccga ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact
    11341 aaaaatcgat gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa
    11401 aaaaaacagt attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta
    11461 gtgaaatcta cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc
    11521 aacggtcttc tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct
    11581 atcgtgacgg gtagcgactc tgaacagtga tgtttcaggg tcagacagga gtagggaagg
    11641 aatacagaga gacaataata atggtagata gcaagaagcg ccctggcaaa gatctcgacc
    11701 gtatcgatcg taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc
    11761 tttctaaacg tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa
    11821 gacaagggtt tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac
    11881 ttctggtatt cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca
    11941 ataccgctgt acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg
    12001 actacctgtt gaaaacacgc gtgccggata tgtcagccta ccgtaagttg ctgggggaaa
    12061 ccctgctgcg tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca
    12121 agcagagtaa tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt
    12181 attttgatta cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt
    12241 gtccgtttta gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga
    12301 atacactgaa gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga
    12361 ggcgttgctg atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt
    12421 taacccttcg gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg
    12481 tgggatgccc ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta
    12541 caccattccc gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga
    12601 cgaaaacatt gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct
    12661 tacctcctgt ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg
    12721 cgtcattggc agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac
    12781 tattgcgctg ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt
    12841 gaccattgct gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg
    12901 tacccgtcgc gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga
    12961 agatgaaaac cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct
    13021 agcgcgtcgt aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc
    13081 tgcgttgttc tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg
    13141 tgtggctgcc gacccggacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata
    13201 tgacgaatac gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc
    13261 tgctgctacc acggcgacac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc
    13321 gcctgttgcc tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc
    13381 gggtccacaa acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagtc
    13441 acaatatgcg cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccgca
    13501 gcagccgtat tatgcacctg cagctgaaca acctgcgcaa cagccgtatt atgcccctgc
    13561 gccagaacaa ccggtggcag gtaacgcctg gcaagccgaa gagcagcaat ccacttttgc
    13621 tccacagtct acataccaga ctgagcaaac ttatcagcag ccagccgctc aggagccgtt
    13681 gtaccaacag ccgcaacccg ttgaacagca gcctgttgtg gagcctgaac ccgttgtaga
    13741 agagacaaaa cccgcgcgtc cgccgcttta ctactttgaa gaagttgaag agaagcgagc
    13801 ccgtgaacgt gaacaacttg cggcctggta tcaaccgatt ccagaaccgg ttaaagaacc
    13861 agaaccgatc aaatcttcgc tgaaagcacc ttctgttgca gcagtacctc cagtagaagc
    13921 cgctgccgct gtttctccgc tggcatctgg cgtgaaaaaa gcgacactgg cgacgggggc
    13981 tgccgcaacc gttgccgcgc cagtcttcag tctggcaaat agcggtggac cgcgtccgca
    14041 ggtcaaagag gggattggcc cgcagttgcc acgaccgaaa cgtatccgcg tgccaactcg
    14101 tcgtgaattg gcgtcttacg gtattaagct gccctcacag cgtgcggcgg aagaaaaagc
    14161 ccgtgaagcc cagcgcaatc agtacgattc tggcgatcag tacaacgatg atgaaatcga
    14221 tgcgatgcag caggatgaac tggcacgtca gttcgcccag acacagcagc aacgctatgg
    14281 cgaacagtat caacatgatg tgcccgtaaa tgcagaagat gcagatgctg cggcagaggc
    14341 tgaactggct cgtcagtttg cgcaaactca acaacaacgt tattccggcg aacaaccggc
    14401 tggggcgaat ccgttctcgc tggatgattt tgaattttcg ccaatgaaag cgttgctgga
    14461 tgatggtcca cacgaaccgc tgtttacgcc aattgttgaa cctgtacagc agccgcaaca
    14521 accggttgca ccgcagcagc agtatcagca gccacaacag ccggttgcgc cacagccgca
    14581 gtatcagcag ccacaacagc aggttgcgcc gcagccacaa tatcagcagc cgcaacaacc
    14641 ggttgcgcca cagcagcaat atcagcagcc gcaacaacca gttgcgccgc agccgcagta
    14701 tcaacagccg caacaaccgg ttgcaccgca gccgcagtat cagcagccac aacagccagt
    14761 tgcgccacag ccgcaggata ctctgcttca tccgctgttg atgcgtaatg gcgacagccg
    14821 tccgttgcat aaaccgacga cgccgctgcc ttctctggat ttgctgacac cgccgccgag
    14881 cgaagtggag ccggtagata cctttgcgct tgaacaaatg gctcgcctgg tggaagcgcg
    14941 tctggctgat ttccgtatta aagccgatgt cgtcaattac tctccggggc cggttatcac
    15001 tcgctttgaa cttaacctgg caccgggcgt aaaagcggcg cgcatttcta acttgtcacg
    15061 ggaccttgct cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa
    15121 accctatgta ggtctggagt taccgaataa aaaacgacaa accgtttatc tgcgcgaagt
    15181 attggataac gccaaattcc gcgataatcc atcgccatta accgtggtgc tgggtaaaga
    15241 tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt tagttgcggg
    15301 gactaccggt tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca tgctttataa
    15361 agcacagcca gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg agctttcggt
    15421 gtatgaaggc attccgcacc tgttaacgga agtcgttact gatatgaaag atgccgccaa
    15481 cgcgctgcgc tggtgtgtta acgagatgga gcgtcgttat aaactgatgt ctgcgctggg
    15541 tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc
    15601 gattccagac ccgtactgga agccgggtga cagtatggat gcccagcatc cggtgctgaa
    15661 aaaagaacca tacattgtgg tgttggtgga cgaatttgcc gacctgatga tgacagtagg
    15721 taaaaaagtg gaagagctga tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca
    15781 cctcgtactg gcaactcagc gtccatcggt tgatgttatt actggtctga ttaaagcgaa
    15841 tattccgacc cgtatcgcct ttaccgtatc cagtaagatt gactcacgta ccattcttga
    15901 tcaggctggc gcggaatcac tgctgggtat gggggatatg ctctactctg ggccgaactc
    15961 cacgttgccg gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc atgccgtggt
    16021 gcaggactgg aaagcgcgtg gtcgcccaca gtatgttgat ggcatcacct ccgacagcga
    16081 aagcgaaggt ggtgcgggtg gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca
    16141 ggcggtgcag tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac aacgtcagtt
    16201 ccgcattggt tataaccgtg cagcgcgtat tatcgaacag atggaagcgc aggggattgt
    16261 cagcgaacag gggcacaacg gtaatcgtga agtgctgtcc ccaccgccgt ttgactaatt
    16321 aatgcattgc cggataaggc gcggtagcgt cgcatccggc actctatcaa ctgaaaattc
    16381 agtattttct tctttcctca agctgattat tagcctggaa tagagagtag agggaactcc
    16441 cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa aattgccatc acctgtgcat
    16501 tactctcaag cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc
    16561 gcctggataa agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg
    16621 gcgcggcggt gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact
    16681 ggcatatgac acaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct
    16741 ataacccgtt cgttgagcaa gctacggcaa cctggctgaa agatgccacc ggtaatacgc
    16801 cgtttatgct gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga
    16861 atggcgatga ctttgtcctg acgccgaaag ccagcaatgg caatctgaag cagttcacca
    16921 ttaacgtggg acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc
    16981 gcagcagtta tcaactgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct
    17041 tcaccccgcc gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc
    17101 aatctgtcgc tcgatttttc ggataatact tttcaacctc tggccgcgcg tatgcggcca
    17161 gaaaatttag cacagtatat cggtcagcaa catttgctgg ctgcggggaa gccgttgccg
    17221 cgcgctatcg aagccgggca tttacattct atgatcctct gggggccacc gggtaccggc
    17281 aaaacaaccc tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct
    17341 gccgtcacct ctggtgtgaa agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc
    17401 aatgcaggtc gccgcactat tctttttgtt gacgaagttc accgtttcaa caaaagccag
    17461 caggatgcat ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact
    17521 gaaaacccgt cgtttgagct taattcggca ctgctttccc gtgcccgtgt ttatctgttg
    17581 aaatccctga gtacagagga tattgagcaa gtactaactc aggcgatgga agacaaaacc
    17641 cgtggctatg gtggtcagga tattgttctg ccagatgaaa cacgacgtgc cattgcagaa
    17701 ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc
    17761 gaagtcgatg atagcggtaa gcgggtcctg aagcctgaat tactgaccga aatcgccggt
    17821 gaacgtagcg cccgctttga taacaaaggc gatcgctttt acgatctgat ttccgcactg
    17881 cataagtcgg tacgtggtag cgcacccgat gcggcgctgt actggtatgc gcgaattatt
    17941 accgctggtg gcgatccgtt atatgtcgcg cgtcgctgtc tggcgattgc gtctgaagac
    18001 gtcggtaatg ccgatccacg ggcgatgcag gtggcaattg cggcctggga ttgctttact
    18061 cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc
    18121 gcgccaaaaa gcaacgctgt ctacactgcg tttaaagccg cgctggccga tgctcgcgaa
    18181 cgcccggatt atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa
    18241 atgggctacg ggcaggaata tcgttacgct catgatgaag caaacgctta tgctgccggt
    18301 gaggtttact tcccgccgga aatagcacaa acacgctatt atttcccgac aaacaggggc
    18361 cttgaaggca agattggcga aaagctcgcc tggctggctg aacaggatca aaatagcccc
    18421 ataaaacgct accgttaatg ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg
    18481 caggctgtgg ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc
    18541 aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag
    18601 ctggatgtag ataagctggg cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg
    18661 gaaaacctgc aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc
    18721 ggggaagata tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca
    18781 gcaaaagccg agctggatgc tttacaggct gaaattcgcg atatcgcgct gaccatccct
    18841 aacctgcctg cagatgaagt gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc
    18901 cgctggggta ccccgcgtga gtttgacttt gaagttcgtg accacgtgac gctgggtgaa
    18961 atgcactctg gcctcgactt tgcagctgca gttaagctga ctggttcccg ctttgtggta
    19021 atgaaagggc agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat
    19081 accgaacagc atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg
    19141 tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt tccatactcg tccgctggaa
    19201 gaagaagcag acaccagtaa ctatgcgctg atcccaactg cagaagttcc gctgactaac
    19261 ctggtgcgcg gtgaaatcat cgatgaagat gatctgccaa ttaagatgac cgcccacacc
    19321 ccatgcttcc gttctgaagc cggttcatat ggtcgtgaca cccgtggtct gatccgtatg
    19381 caccagttcg acaaagttga aatggtgcag atcgtgcgcc cggaagactc aatggcggcg
    19441 ctggaagaga tgactggtca tgcagaaaaa gtcctgcagt tgctgggcct gccgtaccgt
    19501 aaaatcatcc tttgcactgg cgacatgggc tttggcgctt gcaaaactta cgacctggaa
    19561 gtatggatcc cggcacagaa cacctaccgt gagatctctt cctgctccaa cgtttgggat
    19621 ttccaggcac gtcgtatgca ggcacgttgc cgcagcaagt cggacaagaa aacccgtctg
    19681 gttcataccc tgaacggttc tggtctggct gttggtcgta cgctggttgc agtaatggaa
    19741 aactatcagc aagctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac
    19801 ggactggaat atattggcta atacccaatt tttctgaatc taaaaagcgc ctacgggcgc
    19861 tttttttgtc tccctttgat accgaacaat aattactcct cacttacacg taatactact
    19921 ttcgagtgaa aatctaccta tctctttgat tttcaaatta ttcgatgtat acaagcctat
    19981 atagcgatct gctatagaaa taattacaca atacggtttg ttactggaat caatcgtgag
    20041 caagcttgag tgagccatta tgaaaacgaa aatccctgat gcggtattgg ctgctgaggt
    20101 gagtcgccgt ggtttggtaa aaacgacagc gatcggcggc ctggcaatgg ccagcagcgc
    20161 attaacatta ccttttagtc ggattgcgca cgctgtcgat agcgccattc caacaaaatc
    20221 aggcgaaaag gttatctgga gcgcctgtac agttaactgt ggtagtcgct gcccgctacg
    20281 tatgcacgtc gtggacggtg aaatcaaata tgtcgaaacg gacaataccg gcgatgacaa
    20341 ttacgacggc ctgcaccagg ttcgcgcctg cctgcgtggg cgttccatgc gtcgccgtgt
    20401 ctacaatccg gaccgcctga aatatccgat gaaacgagtc ggggcgcgcg gtgaaggcaa
    20461 attcgagcgc attagctggg aagaagccta cgacatcatc gcgaccaata tgcagcgcct
    20521 gatcaaagag tacggcaacg aatccatcta tctgaactat ggcaccggta cgctgggtgg
    20581 caccatgacc cgctcctggc cgccgggaaa taccctggtc gcgcggctga tgaactgctg
    20641 cggcggctat ctgaaccatt acggcgacta ctcctccgcg caaattgcgg aaggtctgaa
    20701 ctatacctac ggcggctggg cagatggcaa cagcccgtcg gatatcgaaa acagtaagct
    20761 ggtagtgctg tttggtaata accctggcga aacgcgaatg agtggcggtg gggtgactta
    20821 ctatcttgaa caggcacgcc agaaatctaa tgcccgcatg atcatcatcg atccgcgcta
    20881 taccgacacc ggtgccgggc gcgaagatga gtggatccct attcgtccgg gaacagatgc
    20941 cgcactggtt aacggtctgg cgtacgtcat gatcactgaa aacctggtgg atcaggcatt
    21001 cctcgataaa tattgcgttg gctacgatga gaaaaccctg ccagccagtg cgccgaaaaa
    21061 tggccactat aaagcttata ttctgggtga agggccagat ggcgtggcta aaacaccgga
    21121 atgggcctcg caaatcaccg gtgttccggc agacaaaatc atcaaactgg ctcgtgaaat
    21181 cggcagtacc aaaccggcgt ttatcagcca gggatggggc ccgcagcgtc acgctaacgg
    21241 tgaaatcgca acccgtgcta tctcgatgct ggcgattctg accggtaacg ttggtattaa
    21301 tggagggaac agcggcgcgc gtgaaggttc atacagttta ccgtttgtcc gtatgccgac
    21361 cttggaaaac ccgatccaga ccagcatttc gatgtttatg tggaccgatg ccattgaacg
    21421 tggcccggaa atgacggcgc tacgtgatgg tgtgcgcggg aaagataagc tggatgtgcc
    21481 gatcaaaatg atctggaact atgccggtaa ctgcctgatt aaccagcatt ctgaaatcaa
    21541 ccgtacccat gaaatccttc aggatgataa gaagtgcgag ctgattgtgg ttatcgactg
    21601 ccacatgacc tcatcggcga aatatgctga catcctgctg cctgactgca ccgcttccga
    21661 acagatggac tttgcactgg atgcatcctg cgggaatatg tcttacgtga ttttcaacga
    21721 tcaggtgatt aaaccgcgct ttgaatgtaa gaccatctat gaaatgacca gcgaactggc
    21781 aaaacgtctt ggcgttgagc aacagtttac tgaaggccgt acccaggaag agtggatgcg
    21841 gcatctgtat gcccagtcgc gggaagcgat tcctgaactg ccaacgtttg aagagttccg
    21901 caagcagggg atctttaaaa agcgcgaccc acaagggcat cacgttgctt ataaagcctt
    21961 ccgtgaagat ccgcaggcaa atccactgac cacgccatcg ggtaaaattg agatttattc
    22021 gcaggcgctg gctgacattg ccgctacctg ggaattgcca gaaggcgatg tgatcgatcc
    22081 actgccgatc tacacgccgg gctttgaaag ttatcaggat ccgctgaaca aacagtatcc
    22141 gctgcagctt acaggtttcc actataaatc tcgcgttcac tcaacttacg gcaacgttga
    22201 tgtgctgaaa gcggcttgcc gtcaggaaat gtggatcaac ccgcttgatg cccaaaaacg
    22261 cggtatccac aacggcgata aagtcaggat ctttaacgat cgtggtgagg ttcatattga
    22321 ggcgaaagtg acgccacgaa tgatgccggg tgtggtcgca ctgggtgaag gtgcctggta
    22381 tgacccggat gcaaaacgtg tcgataaggg tggttgtatt aacgtactga ccactcaacg
    22441 tccgtctcct ctcgctaagg ggaatccgtc acatacaaac cttgttcagg ttgaaaaggt
    22501 gtaaggagta accgatgaca acccagtatg gattttttat tgattccagc cgttgcaccg
    22561 gttgcaaaac ctgcgagctg gcttgtaaag actacaaaga tttgatgcca gaagtcagct
    22621 tccgccgcat ttatgaatat gctggtggcg actggcagga agataacggt gtctggcacc
    22681 agaacgtgtt tgcctactat ctgtcgattt cgtgcaacca ctgcgaagat ccggcttgta
    22741 ctaaagtctg cccgagcggt gcgatgcata aacgtgaaga tggttttgtt gtggtcgatg
    22801 aagatgtgtg cattggctgc cgctactgcc atatggcttg cccgtatggc gcaccgcaat
    22861 ataacgaaac gaaaggccat atgaccaaat gcgatggttg ttatgaccgt gttgcggagg
    22921 gtaaaaagcc gatctgtgtt gaatcttgtc cgctgcgggc gctggatttc ggccctatcg
    22981 acgagctgcg taaaaaacat ggcgatctgg cggccgttgc gccgttgccg cgagctcact
    23041 ttaccaaacc gaatattgtg atcaaaccca atgccaatag ccgcccgacc ggggatacca
    23101 ctggctatct ggcaaacccg aaggaggtgt gagatgggaa gtggatggca tgaatggccg
    23161 ctgatgatct tcacggtctt cgggcaatgt gtggcgggtg gttttatcgt tctggctttg
    23221 gcgctgctta aaggcgacct gcgagcagaa gcccagcagc gtgttatcgc ctgcatgttt
    23281 ggcttatggg tgctgatggg cattggcttt atcgcctcta tgctccatct tggttcaccg
    23341 atgcgcgcct ttaactcgct caaccgggta ggggcttcag cactcagtaa cgaaatcgcc
    23401 agcggttcga tcttttttgc tgtaggcggc atcggctggt tgctggcaat gctgaaaaag
    23461 ctgtcacctg cattgcgtac gctatggctg gtggtgacga tggttcttgg cgtcgtcttt
    23521 gtctggatga tggtgcgtgt gtataacagc attgataccg ttccgacctg gtacagcatc
    23581 tggacgccga tgggcttctt cctgacgatg ttcatgggcg gcccgttact gggttacctg
    23641 ttgctgagtc tggctggcgt cgatggctgg gcgatgcgtc tgctgccagc catttctgta
    23701 ctggcactgg tggtgagtgg cgtgatgtca gtgatgcagg gcacagagct ggcaactatt
    23761 catagttctg tgcagcaggc cgcagcgctg gtgccggact atggtgcgct gatgtcctgg
    23821 cgaatcgtgc ttttggccgt tgccctgtgc ttgtggattg caccacagct aaaaggttat
    23881 cagcctgcgg ttccgctact atccgtctca ttcattctgc ttctggcagg ggaattaatc
    23941 ggtcgcggcg tattctatgg tttgcatatg accgtgggga tggccgtcgc aagctaatca
    24001 gtaacaaccg gggtttcggc cccggttctc ttttatttct gcttcgttaa cgtgtcataa
    24061 ctggtcatca aattacgata atcaggaatg tggttagaga acaacgtcgc cagtccttca
    24121 atatcattac gccagtcacg gtgcagctcg caagccacgc cgaaccatgt catcaattgc
    24181 gcacctgctt gcgacatgcg atcccatgcc gaatgccggg taatttcatt aaaggtccct
    24241 gacgcgtcag tcacaacaaa gacatcaaac ccttcttcaa tggctgaaag cgccgggaat
    24301 gccacgcaaa cttcggttac cacaccggca ataattaact gttttttacc tgtcgctttg
    24361 actgctttta caaaatcttc gttatcccag gcgttaatat tgccagggcg agcaatgtaa
    24421 ggtgcatcgg ggaattgtgc tttcagctct ggaactaatg ggccgttagg accggtttca
    24481 aaactggtgg taagaatagt tggtaagttg aaatacttag ccaggtcacc cagcgccagc
    24541 acgttatttt taaacttatc gggttcgata tcccgtacaa gggaaagtaa accagcctgg
    24601 tgatcaacaa gcaaaacggc agcatcattt ttatcaagac gaacatacgg tttggtcatc
    24661 ctcttctcct ttcgagagtc agtggtacgt ctgaaatcgg tcagacgcga tgcattgctc
    24721 tgaaagcata gacgggaaat atgagtttgc tgtgaccatg aaatttttcg actgaacgaa
    24781 gcgtatgaaa tttgtgttag atcaataaaa acaatcagat gagattaatc atctcttcag
    24841 cattagcgtt atgaatgagt aatataaatc gataataccg ccgctgcgat atgcgcgtat
    24901 tttcacccat tgacaatgtt ttttgcggtg gcatgatgcg catgaaattt gaacttcctc
    24961 acggttttaa ttcatgtcca cgtatacccg gcctgtcatg cttttgctgt ctggcctgct
    25021 tttgttgact ctggcgatcg cggtgttaaa tacactcgtg ccgctttggc tcgcccagga
    25081 acacatgtcc acatggcagg taggcgttgt cagctcatcc tattttaccg gcaaccttgt
    25141 cggtacattg ctgacagggt atgtcattaa acgcattggc tttaaccgca gctattatct
    25201 ggcctccttc atttttgccg ctggctgtgc cggccttggc ctgatgattg gtttctggag
    25261 ctggttggcg tggcgttttg tcgcgggcgt cggctgtgcc atgatttggg tagtcgttga
    25321 gagcgcgctg atgtgcagtg ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat
    25381 gatggtttat tacgtgggaa catttttagg ccagttactg gtcagcaaag tctcaaccga
    25441 gctgatgagc gtattgccgt gggttacagg tttgacgttg gcagggatct taccgctgtt
    25501 gtttacgcgt gtcctgaatc agcaggctga aaaccatgat tcgacgtcaa ttacgtcaat
    25561 gctaaaactc cgtcaggcgc ggcttggcgt gaatggctgc attatctcag gaatcgtgct
    25621 gggatctcta tatggcctga tgccgctgta cctcaatcac aaaggggtga gcaatgccag
    25681 cattggtttc tggatggcgg tactggtcag tgcgggtatc ctcggacaat ggccgattgg
    25741 acgtctggcg gataagtttg gtcgactgct ggtgttgcgt gttcaggtct ttgtcgtcat
    25801 tctcggcagt atcgcgatgc ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg
    25861 tgccgctggc tttacgctat atccggtggc gatggcctgg gcttgtgaga aagttgaaca
    25921 tcatcaactg gtggcgatga accaggcctt actgttgagc tataccgtgg gaagtctgct
    25981 tggcccgtca tttaccgcta tgctaatgca gaatttctcc gataatttat tgtttatcat
    26041 gatcgccagc gtatcgttta tctatttgct gatgctgctt cgcaacgccg gtcatacgcc
    26101 gaaacccgtt gctcacgtgt aaatgaattc aagcaaagtg tgaacttact gtttcacact
    26161 ctgctttttt gtttcttcta tctgacttgc ttttttccaa attttattcg tttaaaaata
    26221 aaatgtgcag caggttataa ttttgcattt cgctatttcc gcacttctta tttgccgcgc
    26281 ataatccctc gttttacaga tgccccttta attttggcga aggatttgtc tatggctggg
    26341 aatgttcagg aaaaacagct gcgatggtac aacattgcgc tgatgtcttt tatcactgtc
    26401 tggggttttg gcaacgttgt taataactat gccaaccagg ggctggtggt tgttttttca
    26461 tgggtgttta tctttgcgct ctatttcaca ccttatgcac taattgttgg tcagttaggc
    26521 tcgaccttca aagatgggaa gggcggggtc agtacctgga ttaaacacac gatgggaccc
    26581 ggattggctt acctcgccgc gtggacctac tgggtggtgc atattcccta tctggcacaa
    26641 aaaccccagg caatactgat tgcgctcggt tgggcgatga aaggcgacgg ttcgctaatc
    26701 aaagaatatt cagtcgtagc gttacagggg ttaacgctgg tgctgtttat cttctttatg
    26761 tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg gttctgtggc agggattgcg
    26821 atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa ccgcgcctgc gattactgaa
    26881 gtgcatattg cgaccacaaa cattacctgg gaaacgttca ttcctcatat cgactttacc
    26941 tacattacca ctatttcaat gctggttttc gcggttggcg gagctgagaa gatttctcct
    27001 tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa aagggatgtt atgcctggcg
    27061 gtgatggttg cggtttgtgc cattctgggc tcactggcga tggggatgat gtttgactcg
    27121 cgtaatatcc cggatgactt aatgactaac ggtcagtatt acgcctttca gaagctgggc
    27181 gagtattaca acatgggtaa tactttaatg gtgatttacg ccattgcgaa taccctggga
    27241 caagtagctg cgctggtatt ctcgattgat gccccgctta aagtgctatt aggcgatgct
    27301 gacagcaaat atattccagc cagtttatgt cgtaccaacg cttctggtac gcccgttaat
    27361 ggctattttc tgaccctggt gctggtggcg atcctgatta tgctgccgac tctcggcatc
    27421 ggtgatatga acaatctcta taaatggctg ttgaatctta actcggtagt gatgccgctg
    27481 cgttatttat gggtatttgt tgcatttatt gcagtcgttc gcctggcgca gaaatataca
    27541 ccagagtatg tctttattcg taataagcct ctggcaatga ccgtcgggat ttggtgtttt
    27601 gcctttactg cctttgcctg tttgacgggg atcttcccga aaatggaagc cttcactgca
    27661 gagtggacct tccagttggc gctgaatgtt gcaacgccgt ttgtgctggt aggattagga
    27721 ctgatattcc cgctgctggc gcgtaaagcg aatagtaaat aattattgtg gtcggtgacg
    27781 ctgactctgg cagatctgac ccgtttttac ataatcgaga aatgcgcgca agccgcagga
    27841 catatactgg cggtttgggt aatacatttg gaagctgtgc tgtaagggcg ttagaaatag
    27901 agctctgaat tatttatgca gcgcggaata aaaaagcggc aactcaataa agttgccgct
    27961 ttacggggaa attagaacat taccttatga ccgtactgct caagaatacc tttcacgcgt
    28021 tccatggttt ctttcttcgg tggtttaaca ccatcgagtt tgtattcttc acccatcgcc
    28081 acccatttgt gtttgcccag ttcgtggtag gggagaagtt cgattttctc aacgttaccc
    28141 atatcacggg taaattcacc gaggcgatgc gctgaatcgt catcgtcaga ccagcctggg
    28201 acaacaacgt agcggatcca caccttcaca tttttattcg ccagatattt ggcgaactcc
    28261 agcgtgcggt ggttggaaac tccaaccaga ttttggtgga tctcgtcgtt catctgtttg
    28321 agatcgagca ttaccaggtc ggttacttcc agcagttcat caatcaccgg atcgtaacga
    28381 cgaacaaaac cgttggtgtc cagacaggta tgaatgcctt cttttttgca ggcgcggaac
    28441 cagtcacgaa caaactcagc ttgcaggatt gcttcaccgc cggatgcggt aacgccgccg
    28501 ccggaagcgt tcataaagtg gcgataggtc accacttcct tcatcaaatc ttcaacggta
    28561 acttctttac cgccatgcgt gtcccaggtg tcgcggttat gacaatacag gcagcgcatc
    28621 aggcagccct ggaaaaaggt gataaagcga atacctgggc cgtctacggt tccacaggat
    28681 tcaaaggagt gaatgcgacc aataactgac attgcggtgt ttctccagat gtggcccatc
    28741 tgaggccgtg ttggtgcgca gctcgaaggc tacgtcgagt ctgttttggc agtcacctta
    28801 aagtatagat agctgacaaa aaaggctcac gtgctaaaaa aggccccact ttcgtggagc
    28861 ctttattgta cgctttttac tgtacgattt cagtcaaatc taattacata gattgagtga
    28921 aggtacgagt aataacgtcc tgctgctgtt ctttagtcag cgagttgaaa cgtactgcgt
    28981 agccagatac acggatggtc agctgcggat atttttccgg attttccatc gcgtcgagca
    29041 gcatttcacg gttcatcacg ttaacgttca ggtgctgacc accttcgatg gatgcttcgt
    29101 ggtggaagta accatccatc agaccagcca ggttggtctt acgaacttcg tcgtctttac
    29161 ccagtgcgtt cggaacgata gagaaggtgt aggagatacc atctttagcg taagcaaacg
    29221 gcagtttagc aacggaagtc agagacgcta cagcaccttt ctggtcacga ccgtgcatcg
    29281 ggttagcacc cggtccgaac ggcgcgccag cacgacgacc gtctggggtg ttacccgttt
    29341 tcttaccata cacaacgtta gaagtgatgg tcagaacaga ctgagtcggg atagcgtcac
    29401 ggtaggtgtg cagtttctga attttcttca tgaaacgttc taccaggtca acagccaggt
    29461 catctacacg cggatcgttg ttaccaaact gcgggtattc gccttcgatt tcgaagtcga
    29521 tagccagacc gtcttcgtca cgaatcggtt taactttcgc atatttgatt gcagacaggg
    29581 agtcagcagc aacggacaga ccagcgatac cacacgccat ggtgcggata acgtcacggt
    29641 cgtgcagcgc catcagagag gcttcgtagc tgtacttgtc gtgcatgtag tgaatgatgt
    29701 tcagtgcagt gatgtactgt ttagccagcc agtccatgaa gtgatccatg cgctccatca
    29761 cttcatcgta gttcaggaga tcgcctttga tcggttcaga cttcggacca acctgcattt
    29821 tcagtttttc gtcaacgccg ccgttgattg cgtacagcat ggttttcgcc aggtttgcac
    29881 gcgcaccgaa gaactgcatt tgtttaccaa cgatcatcgg gcttacgcag caagcgatag
    29941 cgtagtcatc gttgttgaag tccggacgca tcaggtcatc gttctcatac tgcagagaag
    30001 aggtgtcgat ggacacttta gcggcgaatt tcttgaagtt cagcggcagt ttttcagacc
    30061 acagaatggt catgttcggt tccggagacg gacccatggt gtacagggtg ttcaggaaac
    30121 ggaagctgtt tttggtaacc agggtacgac cgtcgaggcc cataccaccg atagattcgg
    30181 ttgcccagat cgggtcgcca gagaacagtt catcgtactc cggagtacgc aggaagcgaa
    30241 ccatacgcag tttcatgacc aggtggtcaa ccatttcctg cgcttcttgt tcggtgatct
    30301 tgccagcttt caggtcacgt tcgatgtaca catccaggaa ggtggaggta cgaccgaagg
    30361 acattgcagc accgttctga gacttaacag cagccaggta gccgaagtaa gtccactgga
    30421 tagcttcctg agcgttggta gccggaccag agatgtcgta gccgtatttc gcagccattt
    30481 ctttcatctg acccagagcg cggtgctgtt cagcgatttc ttcgcgcaga cggatagtct
    30541 gttccaggtt tacgccgttt tccagatcag cctgcagaga ggtgaactga gcgtatttgt
    30601 ctttcatcag gtagtcgata ccgtacagcg caacgcgacg gtagtcaccg atgatacggc
    30661 cacggccata agcatctggc agaccggtca gaacaccgga tttacggcaa cgcaggatgt
    30721 ccggagtgta aacgtcgaac acgccctggt tgtgagtttt acggtattca gtgaagattt
    30781 ttttgatcat cgggtccagt tcgcggttgt acgctttgca ggaaccttcg atcattttga
    30841 taccaccgaa cgggataaga gcacgtttca gcggagcttc agtctgcaga ccaacaactt
    30901 tttccaacgc tttgttgatg tagccagcgt cgtgagaggt gatggtggaa gcaacagcgg
    30961 tgtcgaagtc aactggcgcg tgagtgcggt tttccagttt aacgccttcc attactttgt
    31021 cccacagggt ggtggtcgct tcagtagcgc cagccaggaa ggactcgtca ccctcgtacg
    31081 gagtgtagtt tttctgaatg aagtcacgga cgtttacttc attctgccag tcacctttgg
    31141 taaaaccttc ccaggctgtg gctaactttt cattaagctc ggacatgtaa cacctacctt
    31201 cttaagtgga ttttttattt actgcgtact tcgacaacca ttaatggtga tcgttttcac
    31261 gcaggtaaat gacccagtat gtcaacccaa ccaacaaacc accgccgata atattaccga
    31321 tcgtaaccgg aatcaggtta tcagtgatga agttcatcac ggtcaggtga gaaaaatttt
    31381 ccggtgcaga accgacagcg gtccagaatt ccggagatgc gaagtcgcgg attacaatac
    31441 ccatcgggat cataaacatg tttgcgatac tgtgctcaaa accgctggca acaaacatcg
    31501 cgaccggcag caccatgata aacgctttgt ccatcaggct gcggccagaa taactcatcc
    31561 atactgccag acataccatc aggtttgcca ggataccaag gcagacggcc tcaataaaag
    31621 tatggtgcac tttgtggtcg gcggtttgta ggacgtttag tccccattga ccatttgcgg
    31681 tcatatactc gccggaaagc cacattaaaa gtacaaacag cagtgcgccg accaggttgc
    31741 caaaatagac atttagccag tttttagcca actgacccca ggtgatgcgc ccactcgcct
    31801 tagcaacaac aatcaacact gtggaagtaa agagatcggc tccgcagaca acacaaagaa
    31861 tcagtcccag agagaagcaa atgccgccaa ccagttttgc catgccgaag ggcattgtgc
    31921 ctgtgccagt ggttgctgtg atatagaaga cgaatgcgat tgagatgaaa acaccggcgg
    31981 taatcgccag atagaaagtc ttaagcggat gtttcgttgc tttatagaca cccgcctctt
    32041 cggccacttt ggccattgca gcaggaagta aaagatcaaa agggttgtca gctttcacac
    32101 taactctctc tttattaagt cggcgacgag atactaacaa agcattatag atgagaaatt
    32161 gatatagatc atatctcgcc tggcttatag gccc
//
LOCUS       DAZKFZ010000034        32172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000034 DAZKFZ010000000
VERSION     DAZKFZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            9..308
                     /locus_tag="ACHOQW_001454"
     CDS             9..308
                     /locus_tag="ACHOQW_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572547.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(355..1242)
                     /gene="rarD"
                     /locus_tag="ACHOQW_001455"
     CDS             complement(355..1242)
                     /gene="rarD"
                     /locus_tag="ACHOQW_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP9572548.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1294..1761)
                     /gene="yigI"
                     /locus_tag="ACHOQW_001456"
     CDS             complement(1294..1761)
                     /gene="yigI"
                     /locus_tag="ACHOQW_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP9572549.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1926..2795
                     /gene="pldA"
                     /locus_tag="ACHOQW_001457"
     CDS             1926..2795
                     /gene="pldA"
                     /locus_tag="ACHOQW_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP9572550.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2928..4757
                     /gene="recQ"
                     /locus_tag="ACHOQW_001458"
     CDS             2928..4757
                     /gene="recQ"
                     /locus_tag="ACHOQW_001458"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9572551.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4821..5441
                     /gene="rhtC"
                     /locus_tag="ACHOQW_001459"
     CDS             4821..5441
                     /gene="rhtC"
                     /locus_tag="ACHOQW_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP9572552.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5503..6123)
                     /gene="rhtB"
                     /locus_tag="ACHOQW_001460"
     CDS             complement(5503..6123)
                     /gene="rhtB"
                     /locus_tag="ACHOQW_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP9572553.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLDVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6234..7256
                     /gene="pldB"
                     /locus_tag="ACHOQW_001461"
     CDS             6234..7256
                     /gene="pldB"
                     /locus_tag="ACHOQW_001461"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP9572554.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7264..8064
                     /gene="yigL"
                     /locus_tag="ACHOQW_001462"
     CDS             7264..8064
                     /gene="yigL"
                     /locus_tag="ACHOQW_001462"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP9572555.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8140..9039
                     /gene="bioP"
                     /locus_tag="ACHOQW_001463"
     CDS             8140..9039
                     /gene="bioP"
                     /locus_tag="ACHOQW_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP9572556.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8927..9880)
                     /gene="metR"
                     /locus_tag="ACHOQW_001464"
     CDS             complement(8927..9880)
                     /gene="metR"
                     /locus_tag="ACHOQW_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP9572557.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10116..12377
                     /gene="metE"
                     /locus_tag="ACHOQW_001465"
     CDS             10116..12377
                     /gene="metE"
                     /locus_tag="ACHOQW_001465"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9572558.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKCGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12417..13232)
                     /gene="ysgA"
                     /locus_tag="ACHOQW_001466"
     CDS             complement(12417..13232)
                     /gene="ysgA"
                     /locus_tag="ACHOQW_001466"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP9572559.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13494..14255
                     /gene="udp"
                     /locus_tag="ACHOQW_001467"
     CDS             13494..14255
                     /gene="udp"
                     /locus_tag="ACHOQW_001467"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP9572560.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14396..15823
                     /gene="rmuC"
                     /locus_tag="ACHOQW_001468"
     CDS             14396..15823
                     /gene="rmuC"
                     /locus_tag="ACHOQW_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP9572561.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15918..16673
                     /gene="ubiE"
                     /locus_tag="ACHOQW_001469"
     CDS             15918..16673
                     /gene="ubiE"
                     /locus_tag="ACHOQW_001469"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP9572562.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16687..17292
                     /gene="ubiJ"
                     /locus_tag="ACHOQW_001470"
     CDS             16687..17292
                     /gene="ubiJ"
                     /locus_tag="ACHOQW_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP9572563.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17289..18929
                     /gene="ubiB"
                     /locus_tag="ACHOQW_001471"
     CDS             17289..18929
                     /gene="ubiB"
                     /locus_tag="ACHOQW_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP9572564.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19008..19277
                     /gene="tatA"
                     /locus_tag="ACHOQW_001472"
     CDS             19008..19277
                     /gene="tatA"
                     /locus_tag="ACHOQW_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP9572565.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19281..19796
                     /gene="tatB"
                     /locus_tag="ACHOQW_001473"
     CDS             19281..19796
                     /gene="tatB"
                     /locus_tag="ACHOQW_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP9572566.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19799..20575
                     /gene="tatC"
                     /locus_tag="ACHOQW_001474"
     CDS             19799..20575
                     /gene="tatC"
                     /locus_tag="ACHOQW_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP9572567.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20617..21399
                     /gene="tatD"
                     /locus_tag="ACHOQW_001475"
     CDS             20617..21399
                     /gene="tatD"
                     /locus_tag="ACHOQW_001475"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP9572568.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21396..21884)
                     /gene="rfaH"
                     /locus_tag="ACHOQW_001476"
     CDS             complement(21396..21884)
                     /gene="rfaH"
                     /locus_tag="ACHOQW_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP9572569.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22051..23544
                     /gene="ubiD"
                     /locus_tag="ACHOQW_001477"
     CDS             22051..23544
                     /gene="ubiD"
                     /locus_tag="ACHOQW_001477"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP9572570.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23590..24291
                     /gene="fre"
                     /locus_tag="ACHOQW_001478"
     CDS             23590..24291
                     /gene="fre"
                     /locus_tag="ACHOQW_001478"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP9572571.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24483..25646)
                     /gene="fadA"
                     /locus_tag="ACHOQW_001479"
     CDS             complement(24483..25646)
                     /gene="fadA"
                     /locus_tag="ACHOQW_001479"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP9572572.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25656..27845)
                     /gene="fadB"
                     /locus_tag="ACHOQW_001480"
     CDS             complement(25656..27845)
                     /gene="fadB"
                     /locus_tag="ACHOQW_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP9572573.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28035..29366
                     /gene="pepQ"
                     /locus_tag="ACHOQW_001481"
     CDS             28035..29366
                     /gene="pepQ"
                     /locus_tag="ACHOQW_001481"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP9572574.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29366..29980
                     /gene="yigZ"
                     /locus_tag="ACHOQW_001482"
     CDS             29366..29980
                     /gene="yigZ"
                     /locus_tag="ACHOQW_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP9572575.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30019..31470
                     /gene="trkH"
                     /locus_tag="ACHOQW_001483"
     CDS             30019..31470
                     /gene="trkH"
                     /locus_tag="ACHOQW_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP9572576.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31482..32027
                     /gene="hemG"
                     /locus_tag="ACHOQW_001484"
     CDS             31482..32027
                     /gene="hemG"
                     /locus_tag="ACHOQW_001484"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP9572577.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7481 a         8141 c         8787 g         7763 t
ORIGIN      
        1 gattctatat gggaaatggt aacttttggc agttgttctt gggtaaagaa ccttacagca
       61 aaaaatctga ttttgttaag gcatggttat tgtggtgggc tattaccatc cttgttctat
      121 ttattttaag cctgactacg acattaaatt taagaagtat aggtataggg ttaagttttg
      181 ggttttgtca aatccttggt agatatacga tgtctctagg atggagtaag tactggggag
      241 ttttgggggg atttcctgta acaaatattg ttctttttct tatcttaatt tttttcaaaa
      301 aaagataaat aagtcagtgg gtaggctctt taaacggcct acccatgtta attcttaact
      361 ttttctacgc tgcgtataaa tcgcatccat cacaaaaatt gccagcgcca cccaaataaa
      421 ggtgaaagtc accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag
      481 gaacatcagc gtcgggccaa tgtactggaa aaagcctaac gttgagagac gaaggcgcgt
      541 ggcagcggcg gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag
      601 taaattcagc gacatcgggt tttgccccat atggctggtt gagctgtcgg caatagcaaa
      661 caggtaaatc gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc
      721 ttctaccgca atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag
      781 cgcgataata ggtagcgaac caaaagtcca cagctggact aacacgccac atatcgccag
      841 aatcaccgcc agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac
      901 aatgttcacc agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt
      961 caccgcccag ataaacagta gccagttgcc accaatcagc acggcagaga ctgccagcat
     1021 aaaaattttc tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct
     1081 catcagcacc accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc
     1141 gggcacgtag taaatcaact tgaagtacgc tggggctata ccccaaataa aataagcggc
     1201 aagagcgagt aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta
     1261 attggtaaca gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc
     1321 actggcaata taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt
     1381 gcctgcacgc aacaggctac tggtagcagt aaaacgctcg cctctgcctg ggcgcagata
     1441 atcgacgcga agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact
     1501 gatggtttcg tggcgggtta aggtacttcc tacgcacacc agaccggcgg cgacatccag
     1561 cgccgacgca atgaccccgc cgtgcaaaat gctttgcgcc cagttgccca ccatcattgg
     1621 ctgattttta aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa
     1681 tgcgcggtta aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc
     1741 agcggtcagt acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt
     1801 tatgattctt tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa
     1861 gtatgtaaaa taccccgcag aaaaacattc accttatcaa taattcgtta cggagaacac
     1921 gaccgatgcg gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat
     1981 atgcacaaga ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta
     2041 tcgccaatat gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact
     2101 acctcatcta cacccaaacc agcgatctga acaaagaagc gattgccagt tacgactggg
     2161 cggaaaatgc gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc
     2221 gtgggatttt aggcccgaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc
     2281 aactgtccaa tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt
     2341 tcctcggttt tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg
     2401 ggtataacca cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt
     2461 atactcgcct gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg
     2521 tgggtaatac tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa
     2581 tcggctatca cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg
     2641 gctacggcgg cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata
     2701 ctcaggttta cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg
     2761 tcggtgtggg ggttatgcta aacgatttgt tttgatgaac ggttgagtgg ttggcaaatc
     2821 tggaatccag catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca
     2881 ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg
     2941 aagtgttgaa tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac
     3001 agtttcgccc cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg
     3061 tcgtcatgcc caccggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa
     3121 acggccttac cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac
     3181 tgcaagccaa cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac
     3241 ttgaagtgat gacaggctgc cgcaccgggc aaattcgtct gctttatatc gccccggaac
     3301 gcctgatgct ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg
     3361 ttgatgaagc gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc
     3421 tcggtcagtt gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg
     3481 acgacaccac gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa
     3541 tcagcagttt tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg
     3601 atcagttgat gcgctacgtg caggaacagc gcggtaagtc cggcattatc tactgcaaca
     3661 gccgggcgaa agtagaagac accgctgcgc gcctgcaaag caagggtatt agcgcggcgg
     3721 cctatcatgc cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg
     3781 atgacctgca aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg
     3841 ttcgcttcgt ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg
     3901 gtcgcgccgg gcgtgatggc ctgcctgcgg aagcgatgtt gttttacgat ccagctgata
     3961 tggcgtggct gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc
     4021 gccacaaact caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat
     4081 tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg
     4141 atccgccgaa acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc
     4201 gtgtgaatca gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc
     4261 agcgtatccg cgactatggt catgacaaac tgaaagtcta tggcatgggc cgtgataaaa
     4321 gccatgaaca ttgggtgagc gtgatccgcc agctgattca cctcggcctg gtgacgcaaa
     4381 atattgccca gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg
     4441 aatcctcttt acaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga
     4501 aatcgttcgg cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat
     4561 cgattgccga tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg
     4621 agatggctga acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga
     4681 tgcgcaagct ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg
     4741 gcgacgacga agagtagtca gcagcataaa aaagtgccag gatgaagact ccgtaaacgt
     4801 ttcccccgcg agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg
     4861 tggcgcttat gagtcccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt
     4921 cccgtaaaga agcgatgatg ggcgtgctgg gtattacctg cggcgttatg gtttgggcag
     4981 ggattgcgtt gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga
     5041 ttatggttgg cggtggcctg tatctctgct ggatgggtta ccagatgctg cgcggcgcgc
     5101 tgaaaaaaga ggcggtttct gcacctgcgc cacaggtcga gctggcgaaa agtgggcgca
     5161 gtttcctgaa aggtttactg accaatctcg ctaatccgaa agcgattatc tactttggct
     5221 cggtgttctc attgtttgtc ggtgataacg ttggcactac cgcgcgctgg ggcattttcg
     5281 cgttgattat tgtcgaaacg ctggcgtggt ttactgtggt ggccagcctg tttgccctgc
     5341 cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt
     5401 tatttgccgg atttggcatt catttgatta tttcgcggta atgccagacg cgtcttcaga
     5461 gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga
     5521 tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat
     5581 ctgctttggt cctttaatcc atagagcaat ccgttgagca agggtggcgt aaccgatcat
     5641 cacaataata tcgaccacaa tagtggtgac atcgagcacg atatactgca tcagttgcgg
     5701 ctgttgcggc atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt
     5761 ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag
     5821 cgatttaaga tcaattgcgc cagcggcgcg ccattgctgt attcccagcc aaatcaagta
     5881 agccgcgcct gcccacttca acacttcaaa cgcaatcact gagcgggaaa atagcgtccc
     5941 caaccccacg ccaaccagca caatatgaat cgccagtccg gtctgaagcc cggcaataga
     6001 cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga
     6061 gcctggcgac aggcttaaaa tgattgatgt cagtaggtag gcaaaccacc attctaaggt
     6121 catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt
     6181 gtcaggcacg ctaaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc
     6241 agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc
     6301 tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg
     6361 tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc
     6421 gtattgagag ttacgtaaaa tatgcggaac tggcctacga tttgttccat ttggggtttg
     6481 atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc
     6541 atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcaac
     6601 aggaggtcca gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg
     6661 cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg
     6721 cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact
     6781 gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg
     6841 cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac
     6901 gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg
     6961 aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgccaacgc
     7021 ttctcttgca ggctgaagag gaacgcgtgg tggataaccg cattcatgac cgtttttgtg
     7081 aactccgcac cgccgcgggc catcctgtcg aagggggacg accgctggta attaaaggtg
     7141 cgtaccatga gatccttttt gaaaaggacg caatgcgctc agttgcgctc cacgccatcg
     7201 ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt
     7261 cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccataca
     7321 ttatcccctt acgccaaaga aaccctgaag ctgctcaccg cgcgcggcat caactttgtg
     7381 tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag
     7441 tcttacatga ttacctccaa tggtgcgcgc gttcacgatc tggatggtaa tctgattttt
     7501 gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg
     7561 gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa
     7621 gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc tggattactg
     7681 gagccggaag gcgtcagcaa agtgttcttc acctgcgatt cccatgaaca actgctgccg
     7741 ctggagcagg cgattaacgc tcgttggggc gatcgcgtca acgtcagttt ctctacctta
     7801 acctgtctgg aagtgatggc gggcggcgtt tcaaaaggcc atgcgctgga agcggtggcg
     7861 aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgatgcc
     7921 gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa
     7981 gaccttcatc ccgagctgga agtgattggt actaatgccg acgacgcggt gccgcattat
     8041 ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt
     8101 cgctacaatg gatactcgtt aatcaaagag ttttccattg tggcgctact tatcatcacc
     8161 acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat
     8221 agctattttg cggtgctggt gcgcgttggc ctggcggcac tcgtttttct gccgtttctg
     8281 cgtacccgtg gcaatagcct gaaaacagtc ggcctgtata tgctggtggg cgcgatgcag
     8341 ctcggcgtga tgtatatgct gagtttccgc gcttatctct acctgacggt ttccgaactg
     8401 ctgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag
     8461 cgccgtctgc gctggggata tgcttttagt gccttgttgg cggtgattgg tgccgggatt
     8521 attcgctatg atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc
     8581 aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg
     8641 ccgcagcata acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca
     8701 tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg
     8761 gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcaacc
     8821 caagtagacg ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg
     8881 gttaacctgg ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg
     8941 gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg
     9001 gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac
     9061 gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt
     9121 tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgtggt agcgcggcaa
     9181 tacccatccg cgcggcaacc atctgaatca acaataaggt gttatcgacg cttttcagcg
     9241 acgggctaac gcctgcgggc tggagaaaat gccgccagac atccagtcgg ctacgctgca
     9301 ccggataaat caacagcgtt tcgctggcga gatcttccgg cgtgattcgc gttttcgccg
     9361 ccagtggatg gtcaggtgct aacaccagac gcacttcata gtcgaacatc ggcgaataat
     9421 gcaggccact acgcggcaga atatcggagg tcatcaccag atccagctcg ccctgctgca
     9481 acgcaggctg cgggtcaaac gttacgcccg atttaaaatc catctccacc tgcggccagt
     9541 tcttatggaa attttctaat gcgggcgtca gccactgaat acagctatgg cactcaatgg
     9601 caatgcgcag gcgcgtctgt tgcggttcat tgcaggcttg cagcgcctgg ctaatttgcg
     9661 gcagcacctg gtttgccagt tgcaacagga tttccccctg tggagtaaag cgtagcggct
     9721 ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag
     9781 acagggcgga ttgcgtttga tgcaacgtcg ccgcagcggc tgcgagagag ccgcagttcc
     9841 gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca
     9901 tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg
     9961 gacggctaaa atccttcgtc ttttaaattt atggtgcgtt ggctgcgttt cttcaccccg
    10021 gtcacttact tcagtaagct cccggggatg aataaacttg ccgccttccc taaattcaaa
    10081 atccatagga tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg
    10141 gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagagagt tattgggcgg
    10201 ggaactccac gcgtgaagaa ctgctggcgg tagggcgtga attgcgtgct cgtcactggg
    10261 atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc
    10321 atgtactgac caccagtctg ctgctgggta acgttccggc gcgtcatcag aacaaagatg
    10381 gttcggtaga tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac
    10441 ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg
    10501 agttcgttaa aggccaacag ttcaaactga cctggacgca gctgctggac gaagtggacg
    10561 aggcgctggc gctgggccac aaggtgaaac ctgtgctgct ggggccgatt acctggctgt
    10621 ggctggggaa agtgaaaggt gaacagtttg accgcctgag cctgctgaac gacattctgc
    10681 cggtttatca gcaagtgctg gcagaactgg cgaaatgtgg catcgagtgg gtacagattg
    10741 atgaacccgc gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt
    10801 acgacgcgct ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc
    10861 caaatctcga cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg
    10921 gtaaagatga cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg
    10981 gtctgatcaa tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gtgcaaatta
    11041 aggacattgt cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc
    11101 ccatcgacct gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg
    11161 ccctacaaaa atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg
    11221 cagctctggc agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata
    11281 atccggcggt agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg
    11341 tctatgaagt gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca
    11401 ccacgattgg ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa
    11461 agggcaatct cgacgccaat aactaccgca cgggcattgc ggaacatatc aggcaggcca
    11521 ttgttgaaca ggaacgtttg ggactggatg tgctggtaca tggtgaggcc gagcgtaatg
    11581 acatggtgga atactttggc gagcacctcg acgggtttgt ctttacgcaa aacggttggg
    11641 tacagagcta cggttcccgc tgcgtgaagc caccgattgt tattggtgac gttagtcgcc
    11701 cggcaccgat taccgtggag tgggcgaagt atgcgcaatc tatgactgat aaaccggtga
    11761 aagggatgct gactggcccg gtgaccattc tctgctggtc gttcccgcgt gaagatgtca
    11821 gccgtgaaac catcgccaaa caaattgcgc tggcgctgcg tgatgaagtg gcggacctgg
    11881 aagccgcagg aattggcatc atccagattg atgaaccggc gctgcgcgaa ggtttaccgc
    11941 tgcgtcgtag cgactgggat gcatatctcc agtggggcgt ggaggccttc cgtatcaacg
    12001 ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg
    12061 acatcatgga ttcgattgcg gcactggacg cagacgtcat caccatcgaa acctcgcgtt
    12121 ctgacatgga gttgctggag tcgttcgaag agtttgatta tccgaatgaa atcggtccag
    12181 gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga
    12241 agaaagcggc aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga
    12301 aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga
    12361 acttgcgtcg ggggtaaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac
    12421 agcgacttct tcccaccata ctgcttaaac cattccagca tacgctgcca gccatcttct
    12481 gcagatgcgg catgatagct cgggcgataa tcagcgttga atgcatgccc ggcgtccggg
    12541 tacacgataa tctctgcttt cgcattagca gcccgcagcg cctggcgcat ggtttcaacg
    12601 ctctcctgcg gaatactgtt atccagaccg ccatataagc cgagaaccgg cgcgttaaga
    12661 tcggttgcga tatcaacagg ttgtttcggt gaattcagcg acttatcgcc cgtcagtttg
    12721 ccgtaccacg ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaagtgata
    12781 cgtccacccc agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa
    12841 gcccaactgg cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc
    12901 agaccgctaa gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa
    12961 agttcaggtg cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata
    13021 tgttcatgca cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt
    13081 tgctttggtc tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag
    13141 cccgccacaa tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca
    13201 ggtgcaaatc cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt
    13261 tgcgcaatgt taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt
    13321 ttatgcagtc ctgcagaatg aagagtgatt tatgtgattt gcatcacctt tggtgggtaa
    13381 atttatgcaa cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg
    13441 gtaaagtgtc tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca
    13501 agtctgatgt ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca
    13561 tcgtccctgg cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta
    13621 agctggcatc tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta
    13681 tcgtctgctc taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac
    13741 agctgggcat tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta
    13801 atgtgggtga tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact
    13861 tcgcaccgct ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag
    13921 ctgcgaaatc cattggcgcg acaactcacg ttggcgtgac agcttcttct gataccttct
    13981 acccaggtca ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt
    14041 ctatggaaga gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaaccctgc
    14101 tgaccatgtg tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc
    14161 gcacccagca agagatcccg aatgctgaga cgatgaaaca aactgaaagc caagcggtga
    14221 aaatcgtggt ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga
    14281 cgcgttcggc cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac
    14341 gtttgtacag cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga
    14401 tttctcaatc atggtttacg cagttattgc gttggtgggt gtggcaattg gctggctgtt
    14461 tgccagttat caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt
    14521 cgcggagtta agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg
    14581 cgagttactc aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga
    14641 tctgcgtgaa gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg
    14701 ccagatgatt aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat
    14761 ttttgagcac agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt
    14821 gtcgccgcta cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa
    14881 agaagcacaa gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc
    14941 gcaaatggcc caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca
    15001 gggcaactgg ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg
    15061 gtatgaatat gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga
    15121 tgtcatcgtg cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt
    15181 cgcctatgaa cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga
    15241 acatatcgcg tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct
    15301 gccggggctg cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt
    15361 actggcgctt gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct
    15421 ggttagcccg actacgctgc tggtggcgct gcgcactatc gccaacctgt ggcgttatga
    15481 gcatcaaagc cgcaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat
    15541 gcgtttgttc atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa
    15601 ctatcggcag gcaatgaaaa aactctcttc agggcgcgga aatgtgctgg cgcaggcaga
    15661 agcgtttcgc ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca
    15721 ggcggtgagc caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga
    15781 agcttatcaa cgcgatgatg aatataatca gcagtcgcgc tagcccattg ggagtagtta
    15841 agccgggtag aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt
    15901 gatgagcagg cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga
    15961 ccgtcgcgaa ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa
    16021 aatacgatgt catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca
    16081 cgattgattg cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg
    16141 gcgacctgac agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg
    16201 atatcaatga atccatgctc aaaatgggcc gcgagaagct gcgtaatatc ggtgtgattg
    16261 gcaacgttga gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg
    16321 attgcatcac catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt
    16381 caatgtatcg cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa
    16441 ttatcgagcc gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg
    16501 gctcactggt cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc
    16561 atcccgatca ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact
    16621 actacaatct gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga
    16681 ccggaaatgc cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc
    16741 ctgtatcgct cacccgcgct gaaaacggcc cgctcgcgtc tgttgggcaa agtgctgcgc
    16801 gtggaggtaa aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat
    16861 gtactgggcg aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg
    16921 ccgaaacttc gcgatcgcca gcagcttgcc gccctgattc gcagtggtga gctggaagtg
    16981 cagggcgata ttcaggtggt gcaaaacttc gttgcgctgg caggtctggc agagttcgac
    17041 cctgcggaac tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc
    17101 atgcgcggag gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg
    17161 gaagccatta ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg
    17221 gaagagacgg ctgccgtcga gcgtgctgtt gatgccctga tcaaacggct ggaaaaactg
    17281 gaggctaaat gacgccaggt gaagtacggc gcctatattt catcattcgc acttttttaa
    17341 gctacggact tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc
    17401 gatactcatt attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac
    17461 gactggccct gcaagaactg gggccggtat ggatcaagtt cgggcaaatg ttatcaaccc
    17521 gccgcgatct ttttccgccg catattgccg atcagctggc gttattgcag gacaaagttg
    17581 ctccgtttga tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg
    17641 tagaagcgtg gtttgacgat tttgaaatca agccactggc ttctgcttct atcgcccagg
    17701 ttcataccgc gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg
    17761 atattttgcc ggtcattaaa gcagacctga aacttatcta ccgtctggct cgctgggtgc
    17821 cgcgtttgct gccggatggt cgccgtctac gcccaaccga agtggtgcgc gagtacgaaa
    17881 agaccttgat tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc
    17941 gaaattttga agacagcccg atgctctaca tcccggaggt ttaccctgac tattgtagtg
    18001 aagggatgat ggtgatggag cgtatttacg gcattccggt gtctgatgtt gcggcgctgg
    18061 agaaaaacgg caccaacatg aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc
    18121 aggtctttcg cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct
    18181 atgaacaccc ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa
    18241 acaaagaaga taaacgctat ctggcagaaa actttattgc cttctttaat cgcgactatc
    18301 gcaaagtggc agagctacac gtcgattctg gctgggtgcc accagatacc aacgttgaag
    18361 agttcgaatt tgctattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa
    18421 tttcgtttgg acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag
    18481 tgcagccgca actggtgtta ctccagaaaa ccctgctcta cgtcgaaggg gtaggacgcc
    18541 agctttatcc gcaactcgat ttatggaaaa cggcgaagcc tttcctggag tcgtggatta
    18601 aagatcaggt cggtattcct gcgctggtga gagcatttaa agaaaaagcg ccgttctggg
    18661 tcgaaaaaat gccagaactg cctgaattgg tttacgacag tttgcgccag ggcaagtatt
    18721 tacagcacag tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac
    18781 aatcgcgtta ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg
    18841 tcagccgacc tgaatggggg ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct
    18901 ggtttgtcgg ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac
    18961 gtataatgcg gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca
    19021 gtatttggca gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc
    19081 tcggctccat cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg
    19141 atgatgaacc aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg
    19201 ccgataagca ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg
    19261 ataaagagca ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca
    19321 tcatcggcct cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg
    19381 gctggattcg cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt
    19441 taaaactcca ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc
    19501 tgacgcccga actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc
    19561 gttcctacgt tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc
    19621 cggtggtgaa agataatgaa gctgcgcatg agggtgtaac gcctgccgct gcacaaacgc
    19681 aggccagttc gccggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg
    19741 cggacgctga accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat
    19801 gtctgtagaa gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct
    19861 gaactgcatt atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat
    19921 ctatcacctg gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc
    19981 caccgacgtg gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat
    20041 tctgtcagcg ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa
    20101 gcatgaacgt cgcctggtgg tgccgctgct ggtttccagc tctctgctat tttatatcgg
    20161 catggcgttc gcctactttg tggtctttcc gctggcattt ggcttccttg ccaataccgc
    20221 gccggaaggg gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct
    20281 gtttatggcg tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat
    20341 ggggattacc tcgccagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt
    20401 cgttgtcggg atgttgctga cgccgccgga tgtcttctcg caaacgctgt tggcgatccc
    20461 gatgtactgt ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg
    20521 aaatcgggaa gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca
    20581 accgcccgtc agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg
    20641 accagttcgc aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga
    20701 gttaatgggc tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg
    20761 gcgcgtcagt attcgtcctg ttggtcaacg gcgggcgtac atcctcacga cagcagccag
    20821 tggcaagctg cgactgaaga agcgattatt gagctggccg cgcagccaga agtggtggcg
    20881 attggtgaat gtggtctcga ctttaaccgc aacttttcga cgccggaaga gcaggaacgc
    20941 gcttttgttg cccagctacg cattgccgca gatttaaaca tgccggtatt tatgcactgt
    21001 cgcgatgccc acgagcggtt tatgacatta ctggagccgt ggctggataa actgcctggt
    21061 gcggttcttc attgctttac cggcacacgc gaagagatgc aggcgtgcgt ggcgcatgga
    21121 atttatatcg gcattaccgg ttgggtttgc gatgaacgac gcggactgga gctgcgggaa
    21181 cttttgccgt tgattccggc ggaaaaatta ctgatcgaaa ctgatgcgcc gtatctgctc
    21241 cctcgcgatc tcacgccaaa gccatcatcc cggcgcaacg agccagccca tctgccccat
    21301 attttgcaac gtattgcgca ctggcgtgga gaagatgccg catggctggc tgccaccacg
    21361 gatgctaatg tcaaaacact gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc
    21421 ttcacactgt gcttaatctc tttattaata agattaagca atagcatgga gcgagcctca
    21481 ccatcgggtt cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt aataatcacc
    21541 ttatcacccg gataaggggt tgccggatcg acaatgtctt tcggtttata taccgatagc
    21601 tgatgaatca ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac gaagtggctg
    21661 acaccgcggg tcgcgttgat agtcgtggta tgaatcactt ctggatcaaa ttccacaaac
    21721 aggtagttgg ggaacaatgg ctcactgact gcagtacgtt tgccacgcac gattttttcc
    21781 agggtgatca tcggtgccag gcaattcaca gcctgtcttt cgaggtgttc ctgggcacgt
    21841 tgaagttgcc cgcgcttgca gtacagtaaa taccaggatt gcataatgac tcttatccgt
    21901 ttaatcgggg cgcaaggata gcaaaagctt tacgctaagt taattatatt ccccggtttg
    21961 cgttataccg tcagagttca cgctaattta acaaatttac agcatcgcaa agatgaacgc
    22021 cgtataatgg gcgcagatta agaggctaca atggacgcca tgaaatataa cgatttacgc
    22081 gacttcttga cgttgcttga acagcagggt gagctaaaac gtatcacgct cccggtggac
    22141 ccgcatctgg aaatcactga aattgctgac cgcacgctgc gtgctggtgg gcctgcgctg
    22201 ttgtttgaaa accctaaagg gtactcaatg ccggtgctgt gcaacttgtt cggtacgcca
    22261 aagcgcgtag cgatgggtat gggccaggaa gatgtttcag cactgcgtga agtcggtaaa
    22321 ttattagcat ttctgaaaga accagagccg ccaaaaggtt ttcgcgatct gtttgataag
    22381 ctgccgcagt ttaagcaggt gttaaacatg ccgacaaagc gactgcgcgg tgcaccctgc
    22441 caacaaaaaa tcgtctctgg cgatgacgtc gatctcaacc gtattcccat tatgacctgt
    22501 tggccggaag atgccgcgcc gctgattaca tgggggctaa ccgttacacg tggccctcat
    22561 aaagagcgac agaatctggg catttatcgc cagcaactga ttggtaaaaa caagctgatt
    22621 atgcgttggc tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg tgcggcgcat
    22681 ccaggtgaac gtttcccgat ctctgtggcg ttgggcgctg atccggcaac cattctcggt
    22741 gcagtcacac cagtaccaga tactttgtcg gaatacgcct ttgccggatt gctacgtggc
    22801 accaaaaccg aagtagtgaa gtgtatttcc aatgatctcg aagtgcccgc cagtgcggag
    22861 attgtgctgg aagggtatat cgaacaaggc gaaatggcgc cagaaggacc gtatggtgac
    22921 cacactggtt actataacga agtcgatagt ttcccggtat ttaccgtgac gcatattacc
    22981 cagcgtgaag atgcgattta ccattccacc tataccgggc gtccgccaga tgaacccgcg
    23041 gtactgggag tggcgttgaa cgaagtattt gttcccattc tgcaaaagca gttcccggaa
    23101 attgtcgatt tttacctgcc gccggaaggc tgctcttatc gcctggcggt agtgacaatc
    23161 aaaaaacagt acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc
    23221 cagtttatgt acactaaatt tgtgatcgtt tgcgatgatg acgttaacgc acgcgactgg
    23281 aacgatgtga tttgggcgat taccacccgt atggacccag cgcgggatac tgttctggta
    23341 gaaaatacgc ctattgatta tctggatttt gcctcgcctg tctccgggct gggttcaaaa
    23401 atggggctgg atgccacgaa taaatggccg ggggaaaccc agcgtgaatg gggacgtccc
    23461 atcaaaaaag atccagatgt tgtcgcacat attgacgcca tctgggatga actggctatt
    23521 tttaacaacg gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc gatccgacag
    23581 agaaagcgca tgacaacttt aagctgtaaa gtgacctcgg tagaagctat cacggatacc
    23641 gtatatcgtg tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg tcagtatttg
    23701 atggtagtga tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac gccggatgaa
    23761 aaagggttta tcgagctgca tattggcgct tctgaaatca acctttacgc gaaagcagtc
    23821 atggaccgca tcctcaaaga tcatcaaatc gtggtcgata ttccccacgg agaagcgtgg
    23881 ctgcgcgatg atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg gttctcttat
    23941 gcccgctcga ttttgctgac agcgttggcg cgtaacccaa accgtgatat caccatttac
    24001 tggggcgggc gtgaagagca gcatctgtat gatctctgcg agcttgaggc gctttcgttg
    24061 aagcatcctg gtctgcaagt ggtgccggtg gttgaacaac cggaagcggg ctggcgtggg
    24121 cgtactggca ccgtgttaac ggcggtattg caggatcacg gtacgctggc agagcatgat
    24181 atctatattg ccggacgttt tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag
    24241 cgtaatgcgc gggaagatcg cctgtttggc gatgcgtttg catttatctg agatataaaa
    24301 aaacccgccc ctgacaggcg ggaagaacgg caactaaaac tttattcagt ggcatttaga
    24361 tctatgtcgc atccggcatt cggcacacga tgcctgatgc gacgctggcg cgtcttatca
    24421 ggcctacatt ggtgccggat cggtagaccg gataaggcgt tcacgccgca tccggcaagt
    24481 ggttaaaccc gctcaaacac cgtcgcaata ccctgaccca gaccgataca catcgtcgcc
    24541 agaccaaact gaacgtcttt gtgttccatc agattcagca gcgtggtgct gatacgcgca
    24601 ccggaacaac ccagcggatg acccagcgcg atcgcgccac cgttgaggtt gatcttctcg
    24661 tcaatctgct caattagtcc cagatcttta atacatggca ggatctgcgc ggcaaaggct
    24721 tcattcattt caaacacgcc gatatcactg acagaaagcc ccgctttttt cagcgccagc
    24781 ttcgaggccg gaaccgggcc gtaacccatg atcgatgggt cacaaccaac gaccgccatc
    24841 gaacgcacac gagcgcgcgg cttaagacct aattcatggg cgcggctttc actcatcacc
    24901 agcatggcag ctgcgccatc ggaaagtgca gaagatgtgc ccgccgttac cgtaccgttt
    24961 actggatcaa acgccggacg cagcgtggcg agggcttcca cggtggtttc cgggcgaatc
    25021 acttcgtcgt aattaaactg cttcaggacg ccgtcggcat cgtgaccacc agttgggatg
    25081 atttcatttt taaatgcgcc cgactgcgtg gcggcccagg cgcgggcgtg tgaccgcgcg
    25141 gcaaaggcat cctgcatttc acggctgata ccgtgcatac gcgccagcat ttctgccgtt
    25201 aagcccatca tgcccgccgc tttggcgaca ttgcggctca ggccagggtg aaaatcgacg
    25261 ccgtgactca tcggcacatg gcccatatgc tccacgccgc caaccagaca tgcctgcgca
    25321 tcgccggtca tgatcattcg tgctgcgtca tgcagtgcct gcatggatga accacacaag
    25381 cgattgacag taaccgccgg gacagagtgc ggcacttctg ccagcagcgc cgcgttacgg
    25441 gcgatattaa aaccctgctc cagcgtctgc tgcacacacc cccagtaaat atcgtcgagg
    25501 gccgccgctt ccagcgccgg gttacgcgcc agcaggctac gcattaaatg agcggagaga
    25561 tcttctgcac gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt
    25621 gcatcgacaa tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc
    25681 ggacgggctg gctcaaccgg gggatagtac ggttcgttat gacgcgcttt attacgcaga
    25741 ccttccggca cttcgtacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat
    25801 tttgcgctgc cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc
    25861 aggccgtaga ccagcgccat atccgcttct gccggagtgg cgataatgcc ttcttccaga
    25921 cagcgcacca cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg
    25981 aaatcgcgct tcggctggct cacttctgcc agcaggtcgt caacagcggc gtcttcttct
    26041 ttcttcggct tacctttgct gtcttcttta taatgccaga aaccgaggcc gttcttctga
    26101 ccaaagcggt tggcatcaaa cagcgcgtcg atggcatcgc gataatcttt ctgcatccgc
    26161 tgtgggaagc ccgctgccat gactgcctgg gcgtgatgcg cggtgtcaat gcccacaacg
    26221 tccagcagat atgccggacc cataggccag ccaaactgtt tttccatcac tttgtcgatc
    26281 ttgcggaaat ccgcgccgtc gcgcagcaac tggctgaaac cggcgaaata cgggaacagc
    26341 acgcggttga cgaagaagcc ggggcaatcg ttaaccacaa tcggcgtttt acccatcttg
    26401 ctcgcccagg cgacgacttt cgcaatagtt tcgtcggagc ttttctcgcc gcgaataatt
    26461 tccaccaacg gcattcggtg gaccgggtta aagaagtgca tcccgcagaa gttttccgga
    26521 cgttccagcg cgttggccag ttcgctgata ggaatggttg aagtgttaga cgccagcacg
    26581 gtatccgggc gtactttctg ctcggtttct gccagcacgg cttttttcac tttcgggttt
    26641 tcaacaatcg cttctaccac aacatccacg cgctcaaatc cggcgtagtc gagcgttggg
    26701 tggatcgtgg agatcacgcc agccagtttc agaccatcga tcttgccgcg ctcaagctgc
    26761 ttgttcagta gtttcgcggc ttcagtcatg ccgagggcta acgacttgtc gttaatatct
    26821 ttcataatca ccggcacgcc tttccacgca gactggtaag cgatgccgcc acccataatg
    26881 cctgcgccca gcacggcggc ctgtttcggg gtttcaacgt ctttggtgag tttcttcgct
    26941 ttgcctttta catattgatc gttaaggaaa atgccgacca gtgcgcgagc ttcgttggta
    27001 tgcgccagcg ggacaaagct tttgttttct aggtttaagg cttcttcacg accaaaacga
    27061 gccgcagctt caatggtttt tactgcggtg atgggggccg ggtaatgttt tcctgccgtt
    27121 tgtgcgacca tccctttagc gatggtgaag ctcatggtgg cttcaatctt gctcagtttt
    27181 agcggttcca gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg
    27241 cgtaaaatcg ccatcgcccc ttcaatcagt ttttctgctt tgactacgcc atccaccaga
    27301 ccgattttca gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagcgca
    27361 ctgtcagcgc ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc
    27421 agtttggttt ccggcagacc gatgcgcaga tccggcgtcg ccagacggta atcggtcgcc
    27481 agcacgcatt cgcagccacc gcccagcgca tagccattga cggcagcaat ggtcggcacc
    27541 ggcagatctt ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt
    27601 tcttcaggaa cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct
    27661 gctttgttcg aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg
    27721 gcctcgccga ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca
    27781 aataccagtt cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa
    27841 agcatgtcag tctcctgaat ccgcaaggtg atctggtcgt accagatgca tcgaagtgtg
    27901 tattttgtgt taaaaatatg caaataaaag attaaagaaa tgccgatctg atcacgctcg
    27961 gcagaaatca cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa
    28021 cagaagggta aaaaatggaa tcactggcct cgctctataa aaatcatata gctacattgc
    28081 aggaacgaac tcgcgatgcg ctgacgcgct tcaagctgga tgcgttactt attcactccg
    28141 gcgaactgtt caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat
    28201 tcaaagcgtg ggtgccggta actcaggtgc caaactgctg gctgctggtg gatggcgtga
    28261 ataagccgaa actgtggttc tatctgccgg ttgattactg gcacaacgtc gaaccgctgc
    28321 cgacctcctt ctggactgaa gatgtagaag tgatcgcgct gccgaaagcc gatggcattg
    28381 gtagtctgtt gcctgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg
    28441 cgctgcaact gggtattgag gccagcaata tcaacccgaa aggggttatc gactacctgc
    28501 attactaccg ctccttcaaa accgagtacg agctggcctg tatgcgtgaa gcgcagaaaa
    28561 tggcggtcaa cggtcatcgc gcggcagaag aagcgttccg ttctggcatg agcgagttcg
    28621 atatcaatat tgcctatctg actgcgaccg gtcatcgtga taccgacgta ccttacagca
    28681 acattgtggc actcaacgaa cacgctgcgg tgctgcatta caccaaactg gatcatcagg
    28741 cgtcggaaga gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgccg
    28801 ctgacctgac ccgtacctgg tcggcaaaaa gtgacaacga ttacgcacag ctggtgaaag
    28861 acgtaaatga tgaacaactg gcgctgatcg cgaccatgaa agctggcgtt agctatgtgg
    28921 attaccacat ccagttccat cagcgcatcg ccaaattgct gcgtaaacat caaatcatca
    28981 ccgatatgag tgaagaggcg atggtcgaaa acgatcttac cgggccgttt atgccgcatg
    29041 gtatcggcca tccgctgggc ctgcaggtgc atgacgtcgc cggttttatg caggatgata
    29101 gcggtacgca cctcgcggca ccggcaaaat atccgtacct gcgctgcacc cgtattctcc
    29161 agccgggcat ggtgttaacc atcgaaccgg gtatctactt cattgaatcg ctgctggcac
    29221 cgtggcgtga agggcagttc agcaagcact tcaactggca gaaaattgaa gcactgaaac
    29281 cgttcggcgg cattcgtatc gaagacaacg tggtgatcca cgaaaataac gtggaaaaca
    29341 tgacccggga tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac
    29401 ggtcgttgaa gagatcaaaa aaagccgctt cattacgctg ttggcgcata ccgatggcgt
    29461 tgaggcggcg aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca
    29521 ttgcgtggcg tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga
    29581 cggggagccg gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg gcagcggcgt
    29641 tggggaaatt accgctgtgg tggtgcgcta ctacggcggc atattgcttg gcaccggagg
    29701 gttagtgaaa gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg
    29761 caagacgcca ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga
    29821 agcgttgctg gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct
    29881 gctgcgggtg gcgcttccgg cggcgaaagt ggctgaattt tccgcgaagc tggcggattt
    29941 tagccgtggt tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga
    30001 actaaggaag cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtc
    30061 atcttatttt cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga
    30121 gcgggccgcg cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctgtgg
    30181 tggccgaacc gcaaagagaa gggcgaactg aaatcccgtg aggggtttct gatagtggtg
    30241 ctgttctgga ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg
    30301 aacctcacga ttaccgatgc gttttttgaa tctttctctg gcctgaccac caccggggcc
    30361 actacgctgg tggggctgga ttcgctccct catgccatcc tcttttatcg ccagatgctg
    30421 caatggtttg gcgggatggg gatcatcgtg ttagcggttg cgatactgcc tatcctcggc
    30481 gtgggtggga tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg
    30541 cgcccgcgaa ttgcggaaac ggcgaaaacc ctgtggttga tttatgtctt gctgaccgtc
    30601 gcctgtgcgc tggcgttgtg gtttgccgga atggatgcct ttgatgccat cggccatagc
    30661 tttgcgacta tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgat
    30721 agcccgacta ttaacactat cattgctatc ttcctgctga tctccggctg taactacggt
    30781 ctgcactttt cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt
    30841 cgcatgttta tcggcgtaca gtttacgctg gtggttattt gtaccctcgt actgtggttt
    30901 cataatgtct acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtgtcg
    30961 atggcgacaa ccgccgggtt tacgactgac agcattgccc gctggccgct ctttttgccg
    31021 gtactgcttt tatgttcagc ttttatcggc ggttgtgccg ggtcaacggg cggtggcctg
    31081 aaagtgatcc gcatcctgct gctgtttaag caggggaacc gtgaactgaa acgactggtg
    31141 catccgaacg ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc
    31201 gaagccgttt ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg
    31261 attatcgcca cgggcgtgga tgacttttct gcctttgcct cggttgttgc gacattgaat
    31321 aacctggggc cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct
    31381 aaatggatcc tgattgccaa catgctgttt ggtcgtctcg aggtctttac attgctggtg
    31441 ctctttaccc cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt
    31501 tctcaacaag ggacggacaa acgcgcgaga ttgcctccta cttggcttcg gaactgaaag
    31561 aactggggat ccaggcggat gtcgccaatg tgcaccgcat tgaagaacca cagtgggaaa
    31621 actacgaccg tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc
    31681 aggaatttgt caaaaagcat gcgacgcggc tgaattcgat gccgagcgcg ttttactccg
    31741 taaacctggt ggcgcgtaaa ccggagaagc gcactccaca gaccaacagc tacgcgcgga
    31801 agtttctgat gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc
    31861 gttacccacg ttatcgctgg tacgaccgtt ttatgatcaa gctgattatg aagatgtcag
    31921 gcggtgaaac ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt
    31981 tcgcccgaga aatcgcccat ttaaccgaca aaccgacgct gaaataagca taaagaataa
    32041 aaaatgcgcg gtcagaaaat tattttaaat ttccccttgt caggccggaa taactcccta
    32101 taatgcgcca ccactgacac ggaacaacgg caaacacgcc gccgggtcag cgggattctc
    32161 ctgagaattc cg
//
LOCUS       DAZKFZ010000035        32117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000035 DAZKFZ010000000
VERSION     DAZKFZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(62..1822)
                     /gene="ycaO"
                     /locus_tag="ACHOQW_001485"
     CDS             complement(62..1822)
                     /gene="ycaO"
                     /locus_tag="ACHOQW_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP9572578.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1952..2644
                     /locus_tag="ACHOQW_001486"
     CDS             1952..2644
                     /locus_tag="ACHOQW_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9572579.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2843..3931
                     /gene="serC"
                     /locus_tag="ACHOQW_001487"
     CDS             2843..3931
                     /gene="serC"
                     /locus_tag="ACHOQW_001487"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP9572580.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4002..5285
                     /gene="aroA"
                     /locus_tag="ACHOQW_001488"
     CDS             4002..5285
                     /gene="aroA"
                     /locus_tag="ACHOQW_001488"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9572581.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5454..6218
                     /gene="ycaL"
                     /locus_tag="ACHOQW_001489"
     CDS             5454..6218
                     /gene="ycaL"
                     /locus_tag="ACHOQW_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP9572582.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6391..7074
                     /gene="cmk"
                     /locus_tag="ACHOQW_001490"
     CDS             6391..7074
                     /gene="cmk"
                     /locus_tag="ACHOQW_001490"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9572583.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7185..8858
                     /gene="rpsA"
                     /locus_tag="ACHOQW_001491"
     CDS             7185..8858
                     /gene="rpsA"
                     /locus_tag="ACHOQW_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9572584.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9018..9302
                     /gene="ihfB"
                     /locus_tag="ACHOQW_001492"
     CDS             9018..9302
                     /gene="ihfB"
                     /locus_tag="ACHOQW_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP9572585.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9509..11773
                     /locus_tag="ACHOQW_001493"
     CDS             9509..11773
                     /locus_tag="ACHOQW_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP9572586.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11810..13558
                     /gene="msbA"
                     /locus_tag="ACHOQW_001494"
     CDS             11810..13558
                     /gene="msbA"
                     /locus_tag="ACHOQW_001494"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP9572587.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13555..14541
                     /gene="lpxK"
                     /locus_tag="ACHOQW_001495"
     CDS             13555..14541
                     /gene="lpxK"
                     /locus_tag="ACHOQW_001495"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP9572588.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14578..15810
                     /gene="ycaQ"
                     /locus_tag="ACHOQW_001496"
     CDS             14578..15810
                     /gene="ycaQ"
                     /locus_tag="ACHOQW_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP9572589.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15862..16044
                     /gene="ycaR"
                     /locus_tag="ACHOQW_001497"
     CDS             15862..16044
                     /gene="ycaR"
                     /locus_tag="ACHOQW_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP9572590.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16041..16787
                     /gene="kdsB"
                     /locus_tag="ACHOQW_001498"
     CDS             16041..16787
                     /gene="kdsB"
                     /locus_tag="ACHOQW_001498"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP9572591.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16941..17834
                     /locus_tag="ACHOQW_001499"
     CDS             16941..17834
                     /locus_tag="ACHOQW_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP9572592.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17811..18590)
                     /gene="elyC"
                     /locus_tag="ACHOQW_001500"
     CDS             complement(17811..18590)
                     /gene="elyC"
                     /locus_tag="ACHOQW_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP9572593.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18726..19511
                     /gene="cmoM"
                     /locus_tag="ACHOQW_001501"
     CDS             18726..19511
                     /gene="cmoM"
                     /locus_tag="ACHOQW_001501"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP9572594.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19508..20830
                     /gene="mukF"
                     /locus_tag="ACHOQW_001502"
     CDS             19508..20830
                     /gene="mukF"
                     /locus_tag="ACHOQW_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP9572595.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20811..21515
                     /gene="mukE"
                     /locus_tag="ACHOQW_001503"
     CDS             20811..21515
                     /gene="mukE"
                     /locus_tag="ACHOQW_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP9572596.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21515..25975
                     /gene="mukB"
                     /locus_tag="ACHOQW_001504"
     CDS             21515..25975
                     /gene="mukB"
                     /locus_tag="ACHOQW_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP9572597.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26236..28083
                     /gene="ldtD"
                     /locus_tag="ACHOQW_001505"
     CDS             26236..28083
                     /gene="ldtD"
                     /locus_tag="ACHOQW_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9572598.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28264..28812
                     /locus_tag="ACHOQW_001506"
     CDS             28264..28812
                     /locus_tag="ACHOQW_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP9572599.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28839..29486
                     /gene="gloC"
                     /locus_tag="ACHOQW_001507"
     CDS             28839..29486
                     /gene="gloC"
                     /locus_tag="ACHOQW_001507"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP9572600.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29537..30727)
                     /gene="aspC"
                     /locus_tag="ACHOQW_001508"
     CDS             complement(29537..30727)
                     /gene="aspC"
                     /locus_tag="ACHOQW_001508"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP9572601.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30912..32000)
                     /gene="ompF"
                     /locus_tag="ACHOQW_001509"
     CDS             complement(30912..32000)
                     /gene="ompF"
                     /locus_tag="ACHOQW_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP9572602.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7807 a         7827 c         8640 g         7843 t
ORIGIN      
        1 aaacgccccg taatataact cagacgaatc attaagccta cattacgtag gctatttgat
       61 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacagcg
      121 actgatgtgc agcaaacgcg tgcagatcgc tatctactgg ttgcaagcca taaaacgccg
      181 cttcgccgct cattgccgca ctggcggctt ccacggcatc tgcgccgtac atgcgaacaa
      241 acgcattcag atattgcagc ggctggcgat cttcttcctg cgccagtaat aacaacgttt
      301 gcaggcagcg ataatagttg gcgcgttccg ggctgaatac cgatgagtta aactccatcg
      361 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
      421 ttaattcacc aatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca
      481 gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca
      541 ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca cgtaaatggc
      601 tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag
      661 gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt
      721 tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga
      781 aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
      841 cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga
      901 aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca
      961 cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc
     1021 cgttagcagg attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat
     1081 catatgcgaa gatcgggaaa ccttctgctt ccagtgtttc gatcgcttca accaccgctg
     1141 ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc
     1201 ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttacggg
     1261 tattgccagc agacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat
     1321 aaacggtctg attatcagac tgacgtgtaa acggcagacc gcaaatacca cgatcttcgt
     1381 taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg
     1441 cgcgcagacg ttcatcgagc agcccttctg gcacatcgtc attttcggtc agtgggaacc
     1501 atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat
     1561 cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg
     1621 ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
     1681 tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc
     1741 cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagtgcg gcatctttgc
     1801 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
     1861 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt
     1921 ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc
     1981 atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt
     2041 tgtactggtc tttttgttcc tcaaaatgac cggaagacgc ggtgtgcggc agatgtcgct
     2101 gtttgaagtt ttaatcattc tgacgctggg atcagcggcg ggagatgtgg cgttttatga
     2161 tgatgtgccg atggtcccgg tacttatcgt ctttattact ctggcgttgt tataccgcct
     2221 ggtaatgtgg ttgatggcgc acagtgaaaa actggaagat cttctggaag gcaagccagt
     2281 tgtcattatt gaagatggcg agctggcctg gtcgaaactc aataactcca acatgacgga
     2341 atttgagttc tttatggagc tacgattgcg tggcgtggag cagctggggc aggtacgtct
     2401 ggcgattctc gaaaccaacg ggcaaatcag tgtctatttc tttgaagatg acaaggtgaa
     2461 atcgggttta cttattttac ccagtgattg tacgcagcgt tacaaagtgg tgccggagtc
     2521 ggcggactat gcctgtattc gttgtagtga aatcattcat atgaaagcgg gggaaaaaca
     2581 attatgtccg cgctgtgcaa atccagaatg gacgaaggca agtcgggcaa aacgggtgac
     2641 ctgacagtaa aaacatcggc tttttgctaa taatccgaga gattcttttg tgtgatgcaa
     2701 gccacatttt tgccctcaac ggttttactc attgcgatgt gtgtcactga atgataaaac
     2761 cgatagccac aggaataatg tattacctgt ggtcgcaatc gattgaccgc gggttaatag
     2821 caacgcaacg tggtgagggg aaatggctca aatcttcaat tttagttctg gtccggcaat
     2881 gctaccggta gaggtgctta aacaggctca acaggaactg cgcgactgga acggtcttgg
     2941 tacgtcggtg atggaagtga gtcaccgtgg caaagagttc attcaggttg cagaggaagc
     3001 cgagaaggat tttcgcgatc ttcttaatgt cccctccaac tacaaggtat tattctgcca
     3061 tggcggtggt cgcggtcagt ttgctgcggt accgctgaat attctcggtg ataaaaccac
     3121 cgcagattat gttgatgccg gttactgggc ggcaagtgcc attaaagaag cgaaaaaata
     3181 ctgcacgcct aatgtctttg acgccaaagt gactgttgat ggtctgcgcg cggttaagcc
     3241 aatgcgtgaa tggcaactct ctgataatgc tgcttatatg cattactgcc cgaatgaaac
     3301 catcgacggt atcgccatcg acgaaacgcc agacttcggc aaagatgtgg tggtcgccgc
     3361 cgacttctct tcaaccattc tttcccgtcc gattgacgtc agccgttatg gtgtaattta
     3421 cgctggcgcg cagaaaaata tcggcccggc aggcctgaca atcgtcatcg tccgtgaaga
     3481 tttgctgggc aaagcgaatg tcgcgtgtcc gtcgattctg gattattcca tcctcaacga
     3541 taacggctcc atgtttaaca cgccgccgac atttgcctgg tatctatctg gtctggtctt
     3601 taaatggctg aaagcgaacg gcggtgtagc tgaaatggat aaaatcaatc agcaaaaagc
     3661 agaactgcta tatggggtga ttgataacag cgatttctac cgcaatgacg tggcgaaagc
     3721 taaccgttcg cggatgaacg tgccgttcca gttggcggac agtgcgcttg acaaattgtt
     3781 ccttgaagag tcttttgctg caggccttca tgcactgaaa ggtcaccgtg tggtcggcgg
     3841 aatgcgcgcg tctatttata atgccatgcc gctggaaggc gttaaagcgc tgacagactt
     3901 catggttgag ttcgaacgcc gtcacggtta atgccgaaat tttgcttaat ccccacagcc
     3961 agcctgtggg gtttttattt ctgttgtaga gagttgagtt catggaatcc ctgacgttac
     4021 aacccatcgc tcgtgtcgat ggcactatta atctgcccgg ttccaagagc gtttctaacc
     4081 gcgctttatt gctggcggca ttagcacacg gcaaaacagt attaaccaat ctgctggata
     4141 gcgatgacgt gcgccatatg ctgaatgcat taacagcgtt aggggtaagc tatacgcttt
     4201 cagccgatcg tacacgttgc gaaattatcg gtaacggcgg tccattacac gcagaaggtg
     4261 ccctggagtt gttcctcggt aacgccggaa cggcaatgcg tccgctggcg gcagctcttt
     4321 gtctgggtag caatgatatt gtgctgaccg gtgagccgcg tatgaaagag cgcccgattg
     4381 gtcatctggt ggatgcgctg cgcctgggcg gggcgaagat cacttacctg gaacaagaaa
     4441 attatccgcc gttgcgttta cagggcggct ttactggcgg caacgttgac gttgatggct
     4501 ccgtttccag ccaattcctc accgcactgt taatgactgc gcctcttgcg ccggaagata
     4561 cggtgattcg tattaaaggc gatctggttt ctaaacctta tatcgacatc acactcaatc
     4621 tgatgaagac gtttggtgtt gaaattgaaa atcagcacta tcaacaattt gtcgtaaaag
     4681 gcgggcagtc ttatcagtct ccgggtactt atttggtcga aggcgatgca tcttcggctt
     4741 cttactttct ggcagcagca gcaatcaaag gcggcactgt aaaagtgacc ggtattggac
     4801 gtaacagtat gcagggtgat attcgctttg ctgatgtgct ggaaaaaatg ggcgcgacca
     4861 tttgctgggg cgatgattat atttcctgca cgcgtggtga actgaacgct attgatatgg
     4921 atatgaacca tattcctgat gcggcgatga ccattgctac ggcggcgtta tttgcaaaag
     4981 gcaccaccac gctgcgcaat atctataact ggcgtgttaa agaaaccgat cgcctgtttg
     5041 cgatggcaac agaactgcgt aaagtcggtg cggaagtaga agaggggcac gattacattc
     5101 gtatcactcc accggaaaaa ctgaattttg ccgagatcgc gacatacaat gatcaccgga
     5161 tggcgatgtg tttctcgctg gtggcgttgt cagatacacc agtgacgatt cttgatccca
     5221 aatgtacggc caaaacattt ccggattatt tcgagcagct ggcgcggatt agccaggcag
     5281 cctgaatgaa taacgtccaa taaatagcca aaactttctt tatcaaaacg tcggcacatt
     5341 gccggcgttt tttttcggac cttgtgagtc attttgatta atggtagcgt cgcttgtcaa
     5401 tgtaagttgt tgatacataa tgtttatata tgattaatca acggatgatt cacatgaaga
     5461 atactaaatt actgctggcg attgtgacct ctgcagcatt actgacgggg tgtcaaaata
     5521 cccacggtat tgataccaat atggctatca gctccggttt aaatgcctat aaagcagcaa
     5581 cgttaagcga tgccgatgca aaagcgattg ccaatcaggg ctgtgccgaa atggacagcg
     5641 gcaatcaagt cgcaagtaaa tccagcaagt acggtaaacg tctggcaaaa atcgccaaag
     5701 cattgggtaa caatattaat ggcacgccgg tcaactataa ggtttatatg accagcgacg
     5761 tcaacgcatg ggcgatggcg aacggctgtg ttcgtgttta cagtggcctg atggacatga
     5821 tgaacgataa cgaaattgaa ggcgttctgg gccatgaact gggccacgtc gcgttgggtc
     5881 actcgctggc tgaaatgaaa gcttcttatg cgatcgttgc cgcacgcgat gccatttcag
     5941 ctaccagcgg tgtggcttcc cagctttccc gctcacaatt gggtgatatc gcagaaggcg
     6001 ctatcaatgc gaaatactcc cgcgataaag agtccgaagc agatgatttc tcctttgatc
     6061 tgttgaagaa acgtggcatc agcacccagg ggctggttgg cagctttgaa aaactggcta
     6121 gcctggatgg cggtcgcacc cagtccatgt ttgactctca cccaccatca acagagcgtg
     6181 cgcaacacat ccgtgatcgt atcgcctctg gtaagtaaat cattgtcatc tttcgggctg
     6241 gtcttctgcc agcccgctat aattgcgcaa taaatcccca tctgaataca gacaaaactg
     6301 gtttttgcac acaacgttaa cgatttgtgg cgtcggcgcg tataatgcgc gcggttatgt
     6361 taacggtacg cctgttttaa ggagataaag atgacggcaa ttgccccggt tattaccatt
     6421 gatggcccaa gcggtgcagg gaaaggcacc ttgtgtaagg ctatggcgga agcgttgcaa
     6481 tggcatctgc tggattcggg tgcaatttat cgcgtactgg cactagcggc attacatcac
     6541 catgttgatg ttgcatcgga agatgcgctg gtgccgctgg catcccatct ggatgtacgt
     6601 tttgtgtcaa ccaatggcaa tctggaagtg atcctcgaag gggaagatgt cagcggcgaa
     6661 attcgtactc aggaagtggc gaatgcagct tcacaagtcg cggcattccc acgcgttcgt
     6721 gaagcattat tgcgtcgcca acgcgcgttt cgcgaattac caggcctgat tgccgatggc
     6781 cgcgacatgg gaacggtggt attccctgat gcgccagtga aaattttcct tgacgcctcc
     6841 tcggaagaac gtgcgcatcg ccgcatgcta cagttgcagg agaagggctt tagtgttaac
     6901 tttgagcgcc ttttggccga gatcaaagaa cgcgacgacc gcgatcgtaa ccgagcggta
     6961 gcgccactgg ttccggcagc cgatgcttta gtgttggatt ccaccacctt aagcattgag
     7021 caagtgattg aaaaagcgct acaatacgcg cgccagaaat tggctctcgc ataagcgacc
     7081 gaatttgcag tacccccgtt gcaatggaat gacagcgggt atgttaaaca accccatccg
     7141 gcatggagcc aggtggacgt taaatataaa cctgaagatt aaacatgact gaatcttttg
     7201 ctcaactctt tgaagagtcc ttaaaagaaa tcgaaacccg cccgggttct atcgttcgtg
     7261 gcgttgttgt tgctatcgac aaagacgtag tactggttga cgctggtctg aaatctgagt
     7321 ccgccatccc ggctgagcag ttcaaaaacg cccagggcga gctggaaatc caggtaggtg
     7381 acgaagttga cgttgctctg gacgcagtag aagacggctt cggtgaaact ctgctgtccc
     7441 gtgagaaagc taaacgtcac gaagcctgga tcacgctgga aaaagcttac gaagatgctg
     7501 aaactgttac cggtgttatc aacggcaaag ttaagggcgg cttcactgtt gagctgaacg
     7561 gtattcgtgc gttcctgcca ggttctctgg tagacgttcg tccggtgcgt gacactctgc
     7621 acctggaagg caaagagctt gaattcaaag taatcaagct ggatcagaag cgcaacaacg
     7681 ttgttgtttc tcgtcgtgcc gttatcgaat ccgaaaacag cgcagagcgc gatcagctgc
     7741 tggaaaacct gcaggaaggc atggaagtta aaggtatcgt taagaacctc actgactacg
     7801 gtgcattcgt tgatctgggc ggcgttgacg gcctgctgca catcactgac atggcctgga
     7861 aacgcgttaa gcatccgagc gaaatcgtca acgtgggcga cgaaatcact gttaaagtgc
     7921 tgaagttcga ccgcgaacgt acccgtgtat ccctgggcct gaaacagctg ggcgaagatc
     7981 cgtgggtagc tatcgctaaa cgttatccgg aaggtaccaa actgactggt cgcgtgacca
     8041 acctgaccga ctacggctgc ttcgttgaaa tcgaagaagg cgttgaaggc ctggtacacg
     8101 tttccgaaat ggattggacc aacaaaaaca tccacccgtc caaagttgtt aacgttggcg
     8161 atgtagtgga agttatggtt ctggatatcg acgaagaacg tcgtcgtatc tccctgggtc
     8221 tgaaacagtg caaagctaac ccgtggcagc agttcgcgga aacccacaac aagggcgacc
     8281 gtgttgaagg taaaatcaag tctatcactg acttcggtat cttcatcggc ctggacggcg
     8341 gcatcgacgg cctggttcac ctgtctgaca tctcctggaa cgttgcaggc gaagaagcag
     8401 ttcgtgaata caaaaaaggc gacgaaatcg ctgcagttgt tctgcaggtt gacgcagaac
     8461 gtgaacgtat ctccctgggc gttaaacagc tcgcagaaga tccgttcaac aactgggttg
     8521 ctctgaacaa gaaaggcgct atcgtaaccg gtaaagtaac tgcagttgac gctaaaggcg
     8581 caaccgtaga actggctgac ggcgttgaag gttacctgcg tgcttctgaa gcatcccgtg
     8641 accgcgttga agacgctacc ctggttctga gcgttggcga cgaagttgaa gctaaattca
     8701 ccggcgttga tcgtaaaaac cgtgcaatca gcctgtctgt tcgtgcgaaa gacgaagctg
     8761 acgagaaaga tgcaatcgca actgttaaca aacaggaaga tgcaaacttc tccaacaacg
     8821 caatggctga agctttcaaa gcagctaaag gcgagtaatt ctctgactct tcgggatttt
     8881 tattctgaag tttgttgagt ttacttgaca gattgcaggt ttcgtcctgt aatcaagcac
     8941 taagggcggc tacggccgcc cttaatcaat gcagcaacag cagccgctta atttgccttt
     9001 aaggaaccgg aggaatcatg accaagtcag aattgataga aagacttgcc acccagcaat
     9061 cgcacattcc cgccaagacg gttgaagatg cagtaaaaga gatgctggag catatggcct
     9121 cgactcttgc gcagggcgag cgtattgaaa tccgcggttt cggcagtttc tctttgcact
     9181 accgcgcacc acgtaccgga cgtaatccga agactggcga taaagtagaa ctggaaggaa
     9241 aatacgttcc tcactttaaa cctggtaaag aactgcgcga tcgcgccaat atttacggtt
     9301 aagtttttac tcaaacttga acgagagaaa agcacctgtc gggtgctttt ttcatttatc
     9361 taatctggaa ctggaagctg cctcgcagag ttttgagcag ttttcaccct ttcgttaaat
     9421 tcttctgaat gtgcctcggg gaacgcaaaa ttcccaccag acaaccgctc aacaaagttg
     9481 cacactttcc ataaacaggg aggggtgcat gaaaataacg acagtcggcg tatgcataat
     9541 ttgcggaatt tttccgttgc tgattttgcc ccaattgcct gggacattaa cccttgcgtt
     9601 tctgactctc ttcgcctgcg tactggcatt tatccctgtt aaaaccgtcc gttatatcgc
     9661 gctgacgttg ctgtttttcg tttggggcat attatcagca aagcaaattt tgttggcagg
     9721 agaaacctta actggcgcga cgcaggatgc aattgttgag atcactgcta ctgacggcat
     9781 gaccactcat tacggtcaaa ttacttatct acaaggtcaa cgtatattcc ctgcgccagg
     9841 ccttgtgctg tatggcgaat atcttccgca agcggtttgt gccggacaac tgtggtcaat
     9901 gaaactcaaa gttcgtgccg ttcatggtca acttaatgat ggcggctttg atagccagcg
     9961 ttatgccatt gcccagcatc agccgctcac cggccgcttt ctgcaggcaa gtgttattga
    10021 accgaattgt agcctgcgtg cacagtatct ggcgtcacta caaacaacgc tgcaacccta
    10081 tccgtggaat gcggttattc ttggtttagg tatgggggaa cggttatccg tccctaaaga
    10141 aatcaaaaat atcatgcgtg atactggaac ggcgcattta atggcgatat cgggattgca
    10201 catcgctttt gcggcgttgc tggctgccgg actcattcgc agtggacaaa tttttctgcc
    10261 tgggcgctgg atccactggc aaatgccatt aattggcgga atctgctgtg ctgcttttta
    10321 tgcctggctg acgggaatgc aacctcctgc attgcgtacc gttgtggcgc ttgctacgtg
    10381 gggtatgctt aagttaagtg ggcgacagtg gagtggctgg gatgtatgga tatgttgtct
    10441 ggcggcaatt ttgctgatgg atcctgttgt cattctctcg caaagtttat ggctctctgc
    10501 cgctgcggtc gcggcattga tattttggta tcagtggttt ccctgtcctg agtggcaact
    10561 gccgccggta ttgcgtgcag ttgtttccct catccatctg caactgggaa tcacacttct
    10621 gcttatgccc gtgcaaatcg tcatttttca tggcattagt ctgacctcgt ttattgcaaa
    10681 tctattagca attcccttgg tgacatttat cacggttccg ttgatcctcg ccgcgatggt
    10741 tgtgcattta agcgggccgt taatcctgga tcaagggtta tggtttcttg ccgaccggtc
    10801 tttggcttta cttttcttgg ggttaaagag tttgccagaa gggtggatca acattgctga
    10861 acgttggcaa tggctatcat tttccccatg gttcttactg gtggtatggc gattaaacgc
    10921 ctggcgaacg ttgccagcaa tgtgtgtggc tgtaggcttg ctgatgtgct ggccgctgtg
    10981 gcaaaaacct cgacctgacg agtggcaagt gtacatgctt gatgtcgggc aagggctggc
    11041 aatggtgata gccagaaacg gcaaagcgat tctctatgac acgggactgg cctggcctga
    11101 aggggatagt gggcaacaac tgattatccc ctggctccac tggcataatc ttgaaccgga
    11161 aggcgttatc ctgagtcatg aacatctgga tcaccgggga gggctggact caatattgca
    11221 tacatggccg atgttatgga tcagaagtcc gttaaactgg gaacatcatc agccctgtgt
    11281 gcgtggcgaa gcgtggcaat ggcaaggatt gcgtttcagc gcgcactggc ctttacaagg
    11341 tcgcaacgat aaaggaaata accattcctg tgtggttaag gttgatgacg ggacgaatag
    11401 cattcttcta accggtgata ttgaagcccc agctgaacaa aagatgctaa gccgttactg
    11461 gcagcaagtg caggcaacat tgcttcaggt acctcaccat ggcagtaata cctcatcatc
    11521 attgccatta attcagcgag tgaatggaaa agtggcactc gcatcggcat cgcgctataa
    11581 cgcatggcga ctgccctcta acaaagttaa gcatcgctat caacaacaag gttatacgtg
    11641 gcttgatact cctcatcagg ggcaagtaac ggtcgatttt tcagcgcaag gctggcggat
    11701 tagcagcctc agggagcaaa ttttacctcg ttggtatcat cagtggtttg gcgtgccagt
    11761 ggataacggg tagaatatgc ggctatttca acaaatgctg gttttttgaa tgcataacga
    11821 caaagatctc tctacgtggc agacattccg ccgactgtgg ccaaccattg cgccttttaa
    11881 agcgggtctg atcgtggcgg gcgtagcgtt aatcctcaac gcagccagcg ataccttcat
    11941 gttatcgctc cttaagccac ttcttgatga tggctttggt aaaacagatc gctccgtgct
    12001 ggtgtggatg ccgctggtgg tgatcgggct gatgattttg cgtggtatca ccagctatgt
    12061 ctccagctac tgtatctcct gggtatcagg aaaggtggta atgaccatgc gtcgccgcct
    12121 gtttggtcac atgatgggaa tgccggtttc attctttgac aaacagtcaa cgggtacgct
    12181 gttgtcacgt atcacctacg attccgaaca ggttgcttct tcctcttccg gcgcactgat
    12241 tactgttgtg cgtgaaggtg cgtcgatcat cggcctgttc atcatgatgt tctattacag
    12301 ttggcaattg tcgatcattt tgattgtgct ggcaccgatt gtttcgattg cgattcgcgt
    12361 agtatcgaag cgttttcgca acatcagtaa aaacatgcag aacaccatgg ggcaggtgac
    12421 caccagcgct gaacaaatgc tgaaaggcca taaagaagta ttgattttcg gtggtcagga
    12481 agtggaaacg aaacgcttcg ataaagtcag caaccgaatg cgtcttcagg ggatgaaaat
    12541 ggtttcagcc tcttccatct ctgatccgat cattcagctg atcgcctctt tggcgctggc
    12601 gtttgttctg tatgcggcga gcttcccaag tgtcatggat agcctgactg ccggtacgat
    12661 taccgttgtt ttctcttcaa tgattgcact gatgcgtccg ctgaaatcgc tgactaacgt
    12721 taacgcccag ttccagcgcg gtatggcggc ttgtcagaca ctgtttacca ttctggacag
    12781 tgagcaggag aaagacgaag gtaagcgcgt gatcgagcgt gcgactggcg acgtggaatt
    12841 ccgcaacgtc acctttactt atccgggacg tgacgtaccc gcattgcgta acatcaacct
    12901 gaaaattccg gcagggaaaa cggtcgctct ggttggacgc tctggttcag gtaaatcaac
    12961 catcgccagc ctgatcacgc gtttttacga tattgatgaa ggcgaaatcc tgatggatgg
    13021 tcacgatctg cgcgagtata ccctggcgtc gttgcgtaac caggttgctc tggtgtcgca
    13081 gaatgtccat ctgtttaacg atacggttgc taacaacatt gcttacgcac gaactgaaca
    13141 gtacagccgt gagcaaattg aagaagcggc gcgtatggcc tacgccatgg acttcatcaa
    13201 taagatggat aacggtctcg atacagtgat tggtgaaaac ggcgtactgc tctctggcgg
    13261 tcagcgtcag cgtattgcta tcgctcgagc cttgttgcgt gatagcccga ttctgattct
    13321 ggatgaagct acctcggctc tggataccga atccgaacgt gcgattcagg cggcactgga
    13381 tgagttgcag aaaaaccgta cctctctggt gattgcccac cgcttgtcta ccattgaaaa
    13441 ggcagacgaa atcgtggtcg tcgaggatgg tgtcattgtg gaacgcggta cgcataacga
    13501 tttgcttgag caccgcggcg tttacgcgca acttcacaaa atgcagtttg gccaatgatc
    13561 gaaaaaatct ggtctggtga atcccctttg tggcggctat tgctgccact ctcctggttg
    13621 tatggcctgg tgagtggcgc gatccgtctt tgctataaac taaaactgaa gcgcgcctgg
    13681 cgtgcccccg taccggttgt cgtggttggt aatctcaccg caggcggcaa cggaaaaacc
    13741 ccggtcgttg tctggctggt ggaacagttg caacagcgcg gtattcgcgt gggggtcgta
    13801 tcgcggggat atggtggtaa ggctgaatct tatccgctgt tattgtcggc agataccacc
    13861 acagcacagg cgggtgatga acctgtgttg atttatcaac gcactgatgc gcctgttgcg
    13921 gtttctcccg ttcgttctga tgcggtaaaa gccattctgg cgcaacaccc tgatgtgcag
    13981 atcatcgtaa ccgacgacgg tttacagcat taccgtctgg cgcgtgatgt ggaaattgtc
    14041 gttattgatg gtgtgcgtcg ctttggcaat ggctggtggt tgccggcggg gccaatgcgt
    14101 gagcgagcgg ggcgcttaaa gtcagttgat gcggtaatcg tcaacggcgg tgtcccccgc
    14161 agcggtgaaa tccccatgca tctgctgccg ggtcaggcgg tgaatttacg taccggtacg
    14221 cgttgtgacg ttgctcagct tgaacatgtg gtggcgatgg cagggattgg gcatccgccg
    14281 cgcttttttg ccacgctgaa gatgtgcggc gtacaaccgg aaaaatgtgt accgctggcc
    14341 gatcatcagt ctttgaacca tgcggatgtc agcgcgttgg taagcaccgg gcaaacgctg
    14401 gtaatgactg aaaaagatgc ggtgaaatgc cgggcctttg cagaagaaaa ttggtggtat
    14461 ttgcccgttg acgcacagct ttcaggtgat gaaccagcga aactgcttgc gcaactaacc
    14521 tcgctggctt ctggcaacta gttacgccgc ggcagcgttc gattgatgga gtcatgaatg
    14581 tcgctgccgc acctctccct tgctgatgcg cgtaatcttc accttgccgc gcaaggcctg
    14641 ttaaacaaac cccgccgtcg agcgtcgttg gaggatattc cggcaacgat ctcccgcatg
    14701 tccttgctgc aaatcgatac catcaatatt gttgcccgta gcccatatct ggtgcttttc
    14761 agtcgtctgg gaaattatcc tgcccagtgg ctggatgagt ctctggcgcg tggcgaatta
    14821 atggaatact gggcgcatga agcctgcttt atgccacgca gtgactttcg tcttattcgc
    14881 caccgcatgc tggcacctga aaaaatgggc tggaaataca aagacgcctg gatgcaggaa
    14941 catgaggcgg aaattgcaca gttaattcag catattcatg ataaggggcc ggtacgttca
    15001 gccgattttg agtatcctcg taaaggtgca agcggctggt gggaatggaa gccgcataaa
    15061 cggcatctgg aaggtttatt tactgccgga aaggtgatgg tgattgaacg gcgcaacttc
    15121 cagcgcgttt atgatttaac ccaccgtgtc atgcctgact gggatgatga gcgcgatctc
    15181 gtttcgcaaa cagaagcaga aatcatcatg ctggataaca gtgcgcgtag ccagggaata
    15241 ttccgcgaac agtggctggc agattactat cggctgaaac gtccggcact ggcagcgtgg
    15301 cgcgaagcga gggctgaaca gcggcaaatc attgctgtgc atgttgaaaa attgggcaat
    15361 ctttggctgc atgctgattt gctgccgcta ctcgagcgag cgctggccgg aaagctcact
    15421 gcaacgcaca gcgcggtact ttcgcctttt gatcctgttg tctgggatcg caaacgcgca
    15481 gagcagcttt ttgattttag ctaccggctg gagtgctata ccccagcgcc gaaacgccag
    15541 tatggctatt ttgttctgcc gttattacat cgtgggcaat tagttgggcg aatggatgcc
    15601 aaaatgcatc gccagacagg catccttgaa gttatctctc tgtggttaca ggaaggtatt
    15661 aaaccaacga caacgctgca aaaagggtta cgtcaggcga ttactgattt cgctaactgg
    15721 cagcaggcaa cgcgggtgac attaggacac tgcccgcaag gtctctttac ggattgccgc
    15781 accggctggg aaatagaccc cgtcgcataa atgattatga taagatttat gcattcataa
    15841 cccgatccat ctggaggaac tatggatcat cgtctgcttg aaatcattgc ctgcccggtt
    15901 tgcaacggaa aactttggta taaccaggaa aaacaagaac ttatttgcaa actggacaac
    15961 ctggctttcc cgctgcgcga tggcattcca gtgttgctgg aaacggaagc ccgcgtgctg
    16021 actgctgatg agagtaaatc atgagttttg tggtcattat tcccgcgcgc tacgcgtcca
    16081 cgcgtctgcc cggtaaacca ttggttgata ttaacggcaa acccatgatt gttcatgttc
    16141 ttgaacgcgc gcgcgaatca ggtgccgagc gcatcatcgt ggcaaccgat catgaggatg
    16201 tcgcccgcgc cgttgaagcc gctggcggtg aagtatgtat gacgcgcgcc gatcaccagt
    16261 cagggacaga acgcttggcg gaagttgtcg aaaaatgcgc gttcagcgac gacacggtga
    16321 tcgttaatgt gcagggtgat gaaccgatga tccctgcgac aatcattcgt caggttgctg
    16381 ataacctcgc tcagcgtcag gtgggtatgg cgactctggc ggtgccaatc cacaatgcgg
    16441 aagaagcgtt taacccgaat gcggtgaaag tggttctcga cgctgaaggg tatgcactgt
    16501 acttctcacg cgctaccatt ccgtgggatc gtgatcgttt tgcaaaaggc cttgaaaccg
    16561 ttggcgataa cttcctgcgt catcttggta tttatggcta ccgtgcaggc tttatccgtc
    16621 gttacgtcac ctggcagcca agcccgttag aacacatcga aatgttagag cagcttcgtg
    16681 ttttgtggta cggcgaaaaa atccatgttg ctgtcgcgca tgaagttcct ggcacaggtg
    16741 tggatacccc tgaagatctc gagcgcgttc gcgctgaaat gcgctaattt cacttcacga
    16801 cacttcagcc aattttggga ggagtgtcgt accgttacga ttttcctcaa tttttctttt
    16861 caacaattga tctcattcag gtgacatctt ttatattggc gctcattatg aaagcagtag
    16921 cttttatgag ggtaatctga atggaacagc tgcgtgccga attaagccat ttactgggcg
    16981 aaaaactcag tcgtattgag tgcgtcaatg aaaaagcgga tacggcgttg tgggctttgt
    17041 atgacagcca gggaaaccca atgccgctaa tggcaagaag ctttagtacg cccggaaaag
    17101 cccgacaact ggcatggaaa accaccatgc tggcaagaag tgggactgtc cgtatgccga
    17161 ctatttatgg tgtgatgacg catgaagaac accccggccc tgatgtcctg ctactggagc
    17221 ggatgcgtgg tgtttcggta gaggcaccag cccgaacacc agaacgttgg gaacagctca
    17281 aagaccaaat cgttgaagcc ttactggcct ggcaccgtca ggacagtcgc ggttgcgtcg
    17341 gcgcggtcga caatactcag gaaaatttct ggccctcatg gtaccggcaa catgttgaag
    17401 tgctatggac cacgctcaat cagttcaata acaccggtct gacgatgcag gataagcgga
    17461 tcctgtttcg cactcgcgaa tgtctcccgg cattatttga aggttttaac gacaattgtg
    17521 tgctgattca cggtaacttc tgtttacgca gcatgttgaa agattcgcgc agcgatcagt
    17581 tactggcgat ggtcgggccg ggactaatgc tttgggcacc gcgagaatac gaactgttcc
    17641 gactaatgga taattctctg gcggaagatt tgctctggag ttacctgcaa cgcgcgccag
    17701 tggcggagtc gttcatctgg cggcgttggt tgtatgtgtt atgggatgaa gttgcgcaac
    17761 tggttaatac cggacgattt agtcggcgca acttcgatct ggcatcaaaa tcactcttgc
    17821 cgtggctcgc ctgacgaacc ttttagccac tgccagatgc ggccaagcgt ttcatagcca
    17881 acacgatcgc tatgcatcaa ccagaccgga gaagggattg cccgttccca cgggttaagc
    17941 ggcgagtcga tggccaactg attcgccggg gctggtagtg gatttaaccc ttcctgctga
    18001 aaaaagatca tcgcccgcgg cagatgtgat gcggaggtca ccagcaggaa aggggcgtca
    18061 ccaatcgcct gtttcactgc cgcagcttct tcttcggtat cttttggcaa atccagggtg
    18121 ataatttgct cgcgcggtac gcccagcgat tgcgcaactc tggcacctac ttccgctgta
    18181 ctcaccgtat tggtttttgc tacgcctccc gtgaagatca gttttgaacc cggattttcg
    18241 cgccataaac ggataccctc attcaggcgt ggcagactgt tattgattaa attagagctc
    18301 ggtgcccact gcgggttcca ggtataacca cctccgagca ccacgatata gtccactttt
    18361 tgcgaattat tccatgtcgg ataggtgctt tcgatgggac gcagcaggcg gtcggctacc
    18421 ggttgcaaac tcaataacaa gagtgccagc cacccaatgc taataaaaat cttcccggtt
    18481 ttttgaaaac ggctaaacca cagtagcgcc aggccagcgc caataattaa caacatcagc
    18541 ggaagtggca gcaacatatt gccaatcact tttttcagtg taaaaagcat cctttctggt
    18601 tcctttttta accatatagc aagggatcgc cagcgatatt acaccagacg ggttcattct
    18661 cgccgtggct gtgacaaaat agcgtcttta tcagaaaacg tcttagccaa ccggtggaga
    18721 aagcaatgca ggatcgcaat tttgatgata ttgcggaaaa gttttcccgt aacatttacg
    18781 gcaccaccaa agggcagctt cgacaggcta ttctgtggca ggatctcgat cgcgtgctgg
    18841 cggaaatggg tccgcaaaaa ctgcgtgtgc tggatgcggg cggtggagaa gggcagaccg
    18901 caatcaaaat ggccgagcgc gggcatcagg tcattttatg cgatctttct gcgcagatga
    18961 tcgaccgcgc aaaacaggcg gcagaagcaa aaggtgtgag cgacaacatg caatttatac
    19021 attgcgccgc tcaggatgtt gcttcgcatt tggaaacgcc cgttgatctg atattgttcc
    19081 atgcggtgct cgagtgggtg gctgatcccc gcagcgtatt gcagaccctg tggtcagtgt
    19141 tgcgtccagg cggcgtgttg tcgttaatgt tctacaatgc gcatggtttg ttgatgcata
    19201 acatggtcgc cgggaatttt gattacgtgc aggcgggaat gccgaaaaag aaaaaacgga
    19261 cgctttcgcc agattatcca cgcgacccga cgcaggttta tctgtggctg gaagaagctg
    19321 gttggcaaat tatgggtaag acaggcgttc gcgtgtttca tgattatctg cgcgagaagc
    19381 accagcagcg cgactgctat gaagcattac ttgaattaga aacgcgttat tgccgtcagg
    19441 aaccgtatat taccctgggg cgttatattc atgtcaccgc gcgcaaaccg cagagcaagg
    19501 ataaagtatg agtgaatttt cccagacagt ccccgaactg gttgcctggg ccagaaaaaa
    19561 tgacttctcc atttcgctgc cggtagaccg actctctttt ctgctggcag ttgccacgct
    19621 gaacggcgag cgtctggatg gtgagatgag tgaaggcgag ctggtggatg cattccgcca
    19681 tgtgagtgat gcgtttgagc aaaccagcga aaccatcggc gtgcgcgcca acaacgcgat
    19741 caacgacatg gtgcgtcaac gtctgctgaa ccgctttacc agcgagcagg cagaagggaa
    19801 cgcaatttac cgtctgacgc cgctcggcat cggcattact gactattaca tccgtcagcg
    19861 cgagttttct acgctgcgcc tttctatgca gttgtcgatt gtggcgggtg agctcaaacg
    19921 cgcagcagat gccgccgaag agggcggtga tgaatttcac tggcaccgta atgtctatgc
    19981 gccactgaaa tattcggtag cagaaatttt cgacagtatc gacctgacgc aacgtctgat
    20041 ggacgaacag cagcagcagg tgaaggacga tatcgcccag ttgctgaaca aagactggcg
    20101 ggcggcgatt tccagctgtg aattgttgct ttcggaaact tccggaacgc tgcgtgaatt
    20161 gcaggataca ctggaagcgg caggcgacaa attgcaggct aatctgttgc gcattcagga
    20221 tgcgacgatg actcatgacg atctgcattt cgtcgatcgt ctggtgttcg atctgcagag
    20281 caaactcgac cgtattatca gttggggcca gcaatccatc gatttgtgga ttggctacga
    20341 ccgccacgta cataaattta ttcgtaccgc gatcgatatg gataaaaacc gcgtctttgc
    20401 tcagcggtta cgtcagtcgg tacaaaccta ttttgatgag ccgtgggcgc taagttatgc
    20461 caatgccgat cgtctgctgg atatgcgtga cgaagagatg gcactgcgcg atgaagaagt
    20521 gactggggaa cttccgccag atttggaata cgaagagttt aacgagatcc gcgaacagct
    20581 ggcggcgatc atcgaagaac aacttgccgt gtacaaaacc agacaagtgc cgctggatct
    20641 tggtctggtg gtacgcgaat atctgtcaca gtatccgcgt gcacgtcact ttgacgttgc
    20701 gcgcattgtt attgatcagg cggtacgtct tggcgtagcg caagcagatt tcaccggact
    20761 gccagcgaaa tggcagccga ttaatgatta cggagccaag gtacaggcgc atgtcatcga
    20821 caaatattga acaagtgatg ccggttaagc tggcgcaggc gctggcgaat ccgttatttc
    20881 cggcgctgga cagcgcctta cgttcaggcc gccatattgg cctcgacgaa ctggataatc
    20941 atgcattcct gatggatttt caggaatatc tggaagagtt ttacgcgcgt tataacgttg
    21001 agcttattcg tgcaccggaa gggttcttct atttacgtcc acgttccacc acgctgatcc
    21061 ctcgttccgt cttgtcggaa ctggatatga tggtcgggaa aatcctctgt tatctctatc
    21121 tcagcccgga acggctggcg aatgagggga ttttcaccca gcaggaactg tacgacgaac
    21181 tgctcaccct ggccgatgaa gcgaaactgc tgaaactggt gaacaaccgt tcaaccggtt
    21241 cagacattga ccgtcagaag ttgcaggaaa aagtacgttc ttcgctcaac cgtctgcgtc
    21301 gtttaggtat ggtgtggttt atgggccacg acagcagcaa gttccgcatt accgaatcgg
    21361 tgttccgctt cggagccgat gtgcgtgctg gcgacgatcc ccgtgaagca cagcgtcgcc
    21421 tgattcgtga tggcgaagca atgccgattg aaaatcatct gcaactcaac gatgaaaccg
    21481 aagagaatca gccagatagc ggagaggaag aataatgatt gaacgcggta aatttcgctc
    21541 actgacgctg attaactgga acggcttctt tgcccgaact tttgaccttg acgagctggt
    21601 cacgacgctt tctggcggta acggggcggg taaatccacc accatggcgg cgttcgttac
    21661 ggcgctgatc cccgacctga ccctgctgca tttccgtaac actacggaag ccggggccac
    21721 cagcggttcg cgcgataaag gtctgcacgg taagctgaaa gcgggtgtct gttattcgat
    21781 gctcgacacc attaactcgc gccaccagcg cgtggtggtc ggtgtgcgtc tgcaacaggt
    21841 tgccggacgc gatcgtaaag tggatatcaa accatttgcc attcagggac tgccgatgtc
    21901 ggtgcagccg acacagctgg tgaccgaaac tctgaacgaa cgccaggcgc gcgtgctgcc
    21961 gcttaacgag ctgaaagaca agctcgaggc gatggaaggc gtgcagttta aacagttcaa
    22021 ctccattacc gattaccact cgctgatgtt cgatctgggc atcattgcgc gtcgtctgcg
    22081 ctccgcatct gaccgtagca aattctatcg tctgatcgaa gcttcgctgt atggcgggat
    22141 ctccagtgcc attacccgtt ctctgcgcga ctacctgttg ccggaaaaca gcggcgtgcg
    22201 taaagcgttc caggacatgg aagcggcgct gcgtgaaaac cgcatgacgc tggaagcgat
    22261 tcgtgtcacc cagtcggacc gtgacctgtt taagcatctg atcagcgaag ctaccaacta
    22321 cgtggcggcg gactacatgc gtcacgccaa cgagcgccgt gtccatctcg ataaggccct
    22381 ggagtttcgt cgcgaactac atacttcgcg tcagcaactg gcggcggagc agtacaaaca
    22441 cgtcgatatg gcgcgtgagc tggcagagca caacggtgcc gaaggcgatc tggaagcgga
    22501 ttatcaggcg gccagcgatc acctgaacct ggtgcaaacc gcactgcgtc agcaggagaa
    22561 gatcgaacgc tacgaagcgg atctcgatga gctgcagatc cgtctggaag agcaaaacga
    22621 agtggtggca gaagccatcg aacgccagga agagaacgag gctcgcgcgg aagctgccga
    22681 actggaagtg gacgagctga aaagccagct tgctgactac cagcaggcgc tggacgtcca
    22741 gcaaacgcgc gcgatccagt acaaccaggc gattgctgcg cttaatcgtg ccaaagaact
    22801 gtgccatctg ccggacttaa ccgccgacag cgccgccgaa tggctggaaa ccttccaggc
    22861 gaaagagctg gaagcgactg aaaaaatgct ctctcttgag cagaaaatga gcatggcgca
    22921 aaccgcgcac agccagtttg agcaggctta tcagctggtg gtggcaatca acggcccgct
    22981 ggcgcgtaac gaggcgtggg atgtcgctcg cgaactattg cgcgaagggg tcgatcagcg
    23041 tcacctggca gagcaggttc agccgttgcg gatgcgctta agcgaactgg aacagcgtct
    23101 gcgcgaacag caagaagctg agcgtctgct ggcagatttc tgcaaacgtc agggcaagaa
    23161 ttttgacatt gacgagctgg aagccctgca tcaggagctg gaagcacgca ttgcctctct
    23221 ttccgatagc gtgtctaacg cccgtgaaga gcggatggca ctgcgccagg agcaggaaca
    23281 gctgcagtct cgcattcaga gtttgatgca gcgtgcaccg gtttggctgg cagcgcaaaa
    23341 cagtctcaac cagttgagcg aacagtgcgg cgaagagttc acctccagcc aggacgtcac
    23401 tgaatatctg caacagttgc tggagcgtga gcgcgaggcc attgttgaac gcgatgaagt
    23461 aggcgcgcgc aaaaacgccg tcgatgaaga gatcgaacgt ttaagccagc ctggcggctc
    23521 tgaagatcag cgtctgaacg cgctagcgga gcgttttggt ggtgtgctgc tgtcagaaat
    23581 ttatgacgac gttagcctgg aagatgcgcc gtacttctca gcgctgtatg gcccgtcacg
    23641 ccacgccatc gtggtgccag atctgtcaca ggtaaccgaa cacctggaag gtttgaccga
    23701 ttgcccggaa gatctctatt tgatcgaagg ggatccgcag tcattcgatg acagcgtgtt
    23761 cagcgttgat gagctggaaa aagcggtagt ggtgaaaatc gccgatcgtc agtggcgtta
    23821 ttcacgtttc ccggaagtgc cgctgtttgg tcgtgctgcg cgtgaaagcc gtattgaaag
    23881 cctccatgcc gagcgtgaag tgctttccga acgcttcgcc acgctctcct ttgatgtaca
    23941 gaaaacccag cgtctgcatc aggcgttcag ccgctttatc ggcagtcatc tggcggtggc
    24001 gtttgagtct gacccggaag cagaaatccg tcaactgaac agccgtcgcg tcgaactgga
    24061 gcgggcgttg agtaatcatg aaaatgataa ccagcagcag cgtattcagt ttgagcaggc
    24121 gaaagaaggc gttacggcgc tgaaccgcat tctgccgcgt ctcaacctgt tggctgatga
    24181 cagcctggcg gatcgcgtcg atgaaatccg cgaacgtctg gatgaagccc aggaagccgc
    24241 gcgttttgtt cagcagtttg gcaatcaact ggcgaaactg gaaccgatcg tttcggtatt
    24301 gcagagcgac ccggaacagt tcgaacagtt aaaagaagat tacgcgtact ctcagcagat
    24361 gcagcgcgat gcccgtcagc aggcgtttgc cctgacggaa gtggtgcagc gtcgtgcgca
    24421 ctttagctac tctgactcgg cagaaatgct tagcggtaac agcgatctca acgaaaaact
    24481 gcgtgaacgt ctggaacagg cggaagcgga gcgtacccgc gctcgcgaag cgttgcgcgg
    24541 tcacgcagcg cagttgagtc agtacaacca ggtgctggct tcgctgaaaa gttcttacga
    24601 caccaaaaaa gagctactca atgatctgca acgtgaattg caggatatcg gcgtgcgtgc
    24661 tgatagcggg gcagaagagc gggcacgtat tcgccgtgac gagctgcatg cacaactgag
    24721 caataaccgt tcgcgccgca atcaactgga aaaagcgctc accttctgcg aagcggagat
    24781 ggataacctg acccgcaaac tgcgcaagct ggagcgtgat tactttgaga tgcgcgagca
    24841 ggtagtgacc gcgaaagcgg gctggtgtgc agtcatgcgc atggtgaaag ataacggcgt
    24901 tgagcgccgc ttacaccgtc gtgagctggc ttatctctcc gctgatgatt tgcgttccat
    24961 gtcggataag gcgttaggtg cgctgcgtct ggcggtggcg gataacgaac atctgcgcga
    25021 cgtgctgcgc atgtcggaag atcccaaacg tccggagcgt aaaattcagt tcttcgtggc
    25081 ggtttatcag catctgcgtg aacgtattcg tcaggatatt attcgtaccg atgatccggt
    25141 ggaagctatc gaacagatgg agatcgaact tagccgtctg accgaagagt taacctcccg
    25201 tgaacagaaa ctggcgatca gttcccgcag cgtggcgaac atcattcgca aaaccattca
    25261 gcgcgagcag aaccgtatcc gtatgctcaa ccaggggttg cagaacgtat cgtttggtca
    25321 ggtgaacagc gtgcgcctca acgtgaacgt gcgtgaaacg cacgccatgc tgctggatgt
    25381 gctctctgaa cagcatgagc agcatcagga tctgtttaac agcaaccgtc tgaccttctc
    25441 ggaagcgctg gcgaaattgt atcagcgtct taatccgcag attgatatgg ggcagcgcac
    25501 gccgcagacc attggcgaag aactgctgga ttaccgcaac tatctggaaa tggaagttga
    25561 ggttaaccgt ggttccgatg gctggttgcg cgcagagtct ggtgcattgt cgaccggtga
    25621 ggcgattggt accggtatgt cgattctggt gatggtggta caaagctggg aagatgaatc
    25681 tcgccgcctg cgcggtaaag atatctctcc ttgccgcctg ctgttcctcg atgaagcagc
    25741 gcgactggat gctcgttcta tcgccacgct gtttgaattg tgtgagcgtt tgcaaatgca
    25801 gctcatcatc gcagcgccgg aaaatatcag cccggagaaa ggcaccacct ataaactggt
    25861 gcgtaaagtc ttccagaata ccgaacatgt tcatgtcgtc ggcctgcgag gatttgcgcc
    25921 gcaactccct gaaacgcttc caggaagtga cgaagcgcct tctcaggcga gttaaaatta
    25981 aggcggcagc aatgccgcct tttctttttc cgaaaactcc gtttctgcac taaaaaagtg
    26041 gcacattacg gcgcgctttt ctttaaactt ctttacatta ggttatgtaa aaacgtatcg
    26101 gcgtttatat actgaagata agcctgatga gtaacaggct tgctcgtcat actttcgtga
    26161 gtattggcgt tgtacaggca agtcgtaaaa taacagcctg gctattcaga gtatgataaa
    26221 aacagggggc aagggatgtt gcttaatatg atgtgtggtc gtcggctgtc ggcaatcagt
    26281 ttgtgcctgg ccgtaacatt cgctccactg ttcaatgcgc aggccgatga gcctgaagta
    26341 atccctggcg acagcccggt ggctgtcagt gaacagggcg aggcactgcc gcaggcgcaa
    26401 gccacggcaa taatggcggg gatcctgcca ttgcctgaag gtgcggcaga aaaagcccgc
    26461 acgcaaatcg aatctcaatt acccgcaggt tacaagccgg tttatcttaa ccagcttcaa
    26521 ctgttgtatg ccgcacgcga tatgcaaccc atgtgggaaa accgtgatgc tgttaaagcc
    26581 ttccagcaac agctggcaga ggtggcgatt gccggtttcc agccgcagtt taataaatgg
    26641 gtagagttac tgaccgatcc tggtgttaac gggatggcac gcgacgtggt gctctctgat
    26701 gcgatgatgg gctatctcca tttcattgca aatattccgg tcaaaggcac tcgctggcta
    26761 tatagcagta aaccttatgc gcttgcaacg ccgccgcttt cggtgattaa ccaatggcag
    26821 caggcgctgg ataaaggtca attgcctacg tttgttgcag gactggcacc gcagcatccg
    26881 caatatgcgg tgatgcatga atcgttactg gccttactca gtgacaccaa accgtggccc
    26941 caactgaccg gcaaagcaac gttgcgccca gggcagtgga gtaacgacgt accggcgttg
    27001 cgtgaaatat tgcaacgcac aggcatgttg gacggggggc cgaaaattac tctacctggc
    27061 gatgacacgc caactgacgc ggtagtcagc ccatccgctg ttactgttga aacagcagaa
    27121 actaagccga tggataagca aacgacgtct cgtagtaaac ctgcgcctgc cgttcgcgcc
    27181 gcctacgata atgaactggt ggaagccgtt aaacgttttc aggcatggca aggattgggg
    27241 gcagatggtg ctattggccc ggcaacgcgt gactggttaa acgtaacgcc cgcccagcgt
    27301 gctggcgtgt tggctctcaa catccagcga ttgcgcttgc tgccaacaga gctttctacc
    27361 gggatcatgg ttaacattcc ggcctattcg ctggtctact atcagaacgg caatcaggtg
    27421 ctggattcgc gagtcattgt cggtcgcccc gatcgcaaaa cgccgatgat gagcagtgcc
    27481 cttaacaatg tagtggtaaa cccgccgtgg aacgtacctc caactctggc acgcaaagat
    27541 attctgccga aagtgcgcaa cgatccggga tatctcgaaa gccatggcta tacggtgatg
    27601 cgcggctgga acagcagaga agcgattgac ccatggcagg ttgactggtc tacaatcacg
    27661 gcctcgaatt taccgtttcg cttccaacag gctccaggcc cacggaactc gctggggcgc
    27721 tataaattca atatgccgag ttcagaggcc atttatttgc atgacacgcc gaaccacaat
    27781 ctgttcaagc gtgatacacg cgcattgagc tcaggctgtg tacgagtgaa taaagcttcc
    27841 gatctggcga atatgctgtt gcaggatgca ggctggaatg acaaacgtat ttctgatgcg
    27901 ctgaagcagg gtgatacacg ttacgtcaat attcggcagt cgattccggt gaatctctac
    27961 tacctgacgg cctttgttgg tgcagatggt cgtacccagt atcgtacaga tatttacaat
    28021 tatgatctgc ctgcgcgatc cagctcgcaa atcgtatcga aagcggaaca attaatcagg
    28081 taaatgaagt agttcgggga aatcagttgt cgtaataatt agcatgattg ggggcaattc
    28141 tctgcagccc ccgtcactgc tggggttgag tcatcttgac gtctgcttta cgggcggtta
    28201 aggtgcctct tgtgcgccag aagtgcatat aaacgataac attgacctgt agacttgatt
    28261 atcatggaca aattcgacgc taatcgccgc aaattgctgg cgcttggtgg cgttgcactc
    28321 ggtgccgcca tcctgccgac ccctgcgttt gcaacactct ctaccccacg cccgcgcatt
    28381 ttgacactca ataatcttca taccggagag tcaatcaaag cggagttttt cgatggcaga
    28441 ggctatattc aggaagaatt ggcaaaactt aaccattttt tccgcgatta ccgcgcgaac
    28501 aaaataaagt ccatcgaccc aggattattc gaccagttgt atcgcctgca agggttatta
    28561 ggcacccgca aaccggtgca actcatttcc ggttatcgtt ccattgatac caacaatgaa
    28621 ctacgcgccc gcagccgtgg agtagcgaag aaaagctatc acactaaagg ccaggcgatg
    28681 gatttccata ttgaaggtat cgcgttaagc aatattcgca aagccgcgtt atctatgcgc
    28741 gcaggtggtg taggatatta cccacgtagt aactttgtgc atattgatac cgggccagca
    28801 cggcactggt agtaattgct taacgaaaca ggggcagtat gaactatcgt attattccgg
    28861 tcaccgcatt ctcccagaac tgttcattga tctggtgtga acaaacccgc ctggccgcac
    28921 tggtcgatcc tggcggcgat gcggaaaaaa tcaaacagga agttgacgcc agcggcctga
    28981 cactgatgca gatcctgctg acgcatggtc atctggacca cgttggcgca gcggcggaac
    29041 tggcgcaaca ttacggcgtg ccggtttttg gcccggaaaa agaagatgag ttctggctgc
    29101 aaggcttgcc tgcgcaaagt cgtatgtttg gtctggaaga gtgccagccg ctgacgccag
    29161 atcgttggct gaacgaaggc gataccatca gcatagggaa tgtgacttta caggtgttac
    29221 attgccctgg gcatacgccg ggtcatgtcg tgttttttga tgatcgggca aagctgctga
    29281 tttctggcga tgttattttc aaaggcggag tagggcgcag tgacttcccg cgtggcgatc
    29341 ataatcaact gatttcttca atcaaagata aattgctgcc actgggtgat gacgtgacat
    29401 ttattccggg tcacggacca ttatccacac ttggttatga acgcctgcat aatcccttcc
    29461 tgcaagacga aatgcccgtc tggtaaggca cacataaaaa agcccgcttt tcagcgggct
    29521 tcattgtttt taacgcttac agcactgcca caatcgcttc gcacagcgga gccatgttat
    29581 ctggtgtcat cccggccacg tttacgcgac cagaagcaac agcatacacg ccaaactctt
    29641 cgcgcagacg cagcacttgt tcttttgtca ggccactgaa ggagaacatg ccgttctgtt
    29701 tgatgataaa gctgaagtcg cggtttgcgc ctttttcctg cagcgtattg acgaacaact
    29761 gacgcatacg ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa atcgcacgta
    29821 acgcatcgtt gctcaggatg gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt
    29881 agttagcgcg aatcgccgct ttcatttggc tgaatgcgcg atcaacggtt tcactgtcgg
    29941 cagcaaccag agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg
    30001 aactggcaac aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt
    30061 cttccagacc acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac cagcctttct
    30121 caacggagag ttgtgccagt gtctgccatt gttccagcgt agggtcgata ccggttgggt
    30181 tgtggcagca gccatggaac agcactacgt cgccagcctg agcttcgttc aggctgttaa
    30241 tcagtgcatc gaagtcaagg gtgtggtttt ccgcatcata ataagcgtat tcacgaactt
    30301 ccagatctgc agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca
    30361 ctcgcttaac gctggtattt tttgccagga aatcggcagc tatgcgtagt gcgccagtgc
    30421 cacccggagt ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta cctttaccaa
    30481 acagcagttc ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt
    30541 tggtggtttc attttcgagc agatactgtt cagccttttt cacgctggtc agtaccgggg
    30601 ttttgcccgt ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat
    30661 cggcacgaaa cagatcggcc aggcccagaa tcgggtcggc aggagcggcg gtaatgttct
    30721 caaacatgac gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga
    30781 gatttacaaa attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga
    30841 ttgctctgga aggcataaaa aaaacaggac caaagtcctg ttttttcggc atttaacaaa
    30901 gaggtgtgct attagaactg gtaaacgata cccacagcaa cggtgtcgtc tgaacctacg
    30961 cccagtttgt tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt
    31021 ttgttgaagt agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata
    31081 ccttctacgt ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatcg
    31141 aactggtatt gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gctgatgttt
    31201 gtaaatttat tagtgatcgg cgtagcgtta cgcgtttcac cgtagttcgc tgccaggtag
    31261 atgttgttcg catcatactt cagaccagta gcccactgtt cagctttttt accgttgcca
    31321 agaagttgag cttcttgcag gttggtacgg tcagctgcac cataagcacc aacgatacca
    31381 aagccttcgt attcgtagct gatagaaccg ccaacaccgt cgccgttaga gcggcgtgca
    31441 gtgtcacgct cgtttttacc caggtactga acagcgaagt tcaggccatc aaccagacca
    31501 aagaagttgg agttacgata ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg
    31561 ctgtatgcag tatcaccacc aaattctggc agcatatcgg tgtaacccag tgcatcataa
    31621 accacaccgt agttacggcc gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg
    31681 aatgccagac gcgttttgtt accagtttga gcgtcagcgc cttcagagtt gttaccctgg
    31741 aagttatatt cccactgacc ataaccggtc agatcggaat tgatttgagt ttccccttta
    31801 aaaccaagac gggcataggt catgtcgcca ttgccaccgt aactgttttc accgttaccc
    31861 ttggagaaat aatgcagacc gacagcttta ccgtacagat ctactttgtt gccatctttg
    31921 ttatagattt ctgcagcgtt tgcagtacct gctactaaca gagcagggac gatcactgcc
    31981 agaatattgc gcttcatcat tatttattac cctcatggtt ttttttatga cacctgccac
    32041 tgccgtcaat aatttctgtc aataaaattt tacggaacta ttgatgagag tttggtgtct
    32101 ttatgtgtct gcaggca
//
LOCUS       DAZKFZ010000036        31215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000036 DAZKFZ010000000
VERSION     DAZKFZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(103..366)
                     /gene="cedA"
                     /locus_tag="ACHOQW_001510"
     CDS             complement(103..366)
                     /gene="cedA"
                     /locus_tag="ACHOQW_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP9572603.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            549..2810
                     /gene="katE"
                     /locus_tag="ACHOQW_001511"
     CDS             549..2810
                     /gene="katE"
                     /locus_tag="ACHOQW_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP9572604.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2857..3615)
                     /gene="chbG"
                     /locus_tag="ACHOQW_001512"
     CDS             complement(2857..3615)
                     /gene="chbG"
                     /locus_tag="ACHOQW_001512"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP9572605.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3628..4980)
                     /gene="celF"
                     /locus_tag="ACHOQW_001513"
                     /gene_synonym="chbF"
     CDS             complement(3628..4980)
                     /gene="celF"
                     /locus_tag="ACHOQW_001513"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9572606.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5086..5928)
                     /gene="chbR"
                     /locus_tag="ACHOQW_001514"
     CDS             complement(5086..5928)
                     /gene="chbR"
                     /locus_tag="ACHOQW_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP9572607.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5936..6286)
                     /gene="chbA"
                     /locus_tag="ACHOQW_001515"
     CDS             complement(5936..6286)
                     /gene="chbA"
                     /locus_tag="ACHOQW_001515"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP9572608.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6337..7695)
                     /gene="chbC"
                     /locus_tag="ACHOQW_001516"
     CDS             complement(6337..7695)
                     /gene="chbC"
                     /locus_tag="ACHOQW_001516"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP9572609.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7780..8100)
                     /gene="chbB"
                     /locus_tag="ACHOQW_001517"
     CDS             complement(7780..8100)
                     /gene="chbB"
                     /locus_tag="ACHOQW_001517"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP9572610.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8399..8737)
                     /gene="osmE"
                     /locus_tag="ACHOQW_001518"
     CDS             complement(8399..8737)
                     /gene="osmE"
                     /locus_tag="ACHOQW_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP9572611.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8939..9766
                     /gene="nadE"
                     /locus_tag="ACHOQW_001519"
     CDS             8939..9766
                     /gene="nadE"
                     /locus_tag="ACHOQW_001519"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9572612.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            9996..10883
                     /gene="cho"
                     /locus_tag="ACHOQW_001520"
     CDS             9996..10883
                     /gene="cho"
                     /locus_tag="ACHOQW_001520"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP9572613.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10843..11418)
                     /gene="ves"
                     /locus_tag="ACHOQW_001521"
     CDS             complement(10843..11418)
                     /gene="ves"
                     /locus_tag="ACHOQW_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP9572614.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11621..12106)
                     /gene="spy"
                     /locus_tag="ACHOQW_001522"
     CDS             complement(11621..12106)
                     /gene="spy"
                     /locus_tag="ACHOQW_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP9572615.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12436..13404)
                     /gene="astE"
                     /locus_tag="ACHOQW_001523"
     CDS             complement(12436..13404)
                     /gene="astE"
                     /locus_tag="ACHOQW_001523"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP9572616.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13397..14740)
                     /gene="astB"
                     /locus_tag="ACHOQW_001524"
     CDS             complement(13397..14740)
                     /gene="astB"
                     /locus_tag="ACHOQW_001524"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP9572617.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14737..16215)
                     /gene="astD"
                     /locus_tag="ACHOQW_001525"
     CDS             complement(14737..16215)
                     /gene="astD"
                     /locus_tag="ACHOQW_001525"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP9572618.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGFPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16212..17246)
                     /gene="astA"
                     /locus_tag="ACHOQW_001526"
     CDS             complement(16212..17246)
                     /gene="astA"
                     /locus_tag="ACHOQW_001526"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP9572619.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17243..18463)
                     /gene="astC"
                     /locus_tag="ACHOQW_001527"
     CDS             complement(17243..18463)
                     /gene="astC"
                     /locus_tag="ACHOQW_001527"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP9572620.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            18909..19715
                     /gene="xthA"
                     /locus_tag="ACHOQW_001528"
     CDS             18909..19715
                     /gene="xthA"
                     /locus_tag="ACHOQW_001528"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9572621.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19882..20592
                     /gene="ydjX"
                     /locus_tag="ACHOQW_001529"
     CDS             19882..20592
                     /gene="ydjX"
                     /locus_tag="ACHOQW_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9572622.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            20597..21274
                     /gene="ydjY"
                     /locus_tag="ACHOQW_001530"
     CDS             20597..21274
                     /gene="ydjY"
                     /locus_tag="ACHOQW_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP9572623.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            21289..21996
                     /gene="ydjZ"
                     /locus_tag="ACHOQW_001531"
     CDS             21289..21996
                     /gene="ydjZ"
                     /locus_tag="ACHOQW_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9572624.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            21996..22544
                     /gene="ynjA"
                     /locus_tag="ACHOQW_001532"
     CDS             21996..22544
                     /gene="ynjA"
                     /locus_tag="ACHOQW_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9572625.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22554..23720
                     /gene="ynjB"
                     /locus_tag="ACHOQW_001533"
     CDS             22554..23720
                     /gene="ynjB"
                     /locus_tag="ACHOQW_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9572626.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23693..25228
                     /gene="ynjC"
                     /locus_tag="ACHOQW_001534"
     CDS             23693..25228
                     /gene="ynjC"
                     /locus_tag="ACHOQW_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9572627.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25228..25881
                     /gene="ynjD"
                     /locus_tag="ACHOQW_001535"
     CDS             25228..25881
                     /gene="ynjD"
                     /locus_tag="ACHOQW_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9572628.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            25948..27255
                     /gene="ynjE"
                     /locus_tag="ACHOQW_001536"
     CDS             25948..27255
                     /gene="ynjE"
                     /locus_tag="ACHOQW_001536"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP9572629.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27264..27884)
                     /gene="ynjF"
                     /locus_tag="ACHOQW_001537"
     CDS             complement(27264..27884)
                     /gene="ynjF"
                     /locus_tag="ACHOQW_001537"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9572630.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            27971..28378
                     /gene="nudG"
                     /locus_tag="ACHOQW_001538"
     CDS             27971..28378
                     /gene="nudG"
                     /locus_tag="ACHOQW_001538"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP9572631.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28344..28616)
                     /gene="ynjH"
                     /locus_tag="ACHOQW_001539"
     CDS             complement(28344..28616)
                     /gene="ynjH"
                     /locus_tag="ACHOQW_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP9572632.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            28852..30195
                     /gene="gdhA"
                     /locus_tag="ACHOQW_001540"
     CDS             28852..30195
                     /gene="gdhA"
                     /locus_tag="ACHOQW_001540"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9572633.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30312..>31215)
                     /locus_tag="ACHOQW_001541"
     CDS             complement(30312..>31215)
                     /locus_tag="ACHOQW_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9572634.1"
                     /translation="TCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAG
                     FKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTF
                     QYHVLPFDSIDIISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPE
                     QVIALLNKFAVFPSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEEL
                     AWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFW
                     EHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         7719 a         8201 c         7690 g         7605 t
ORIGIN      
        1 tcaaaggcag ctttttgtta caggtggttt gaatgaatgt agcaatgaaa tagagaattt
       61 caggccatgt aactcccggc aaaaccggga ggtacgtaat ctttactcag tcacttcccc
      121 ttcctggcgg atctgatttg cccaacgctg ggcggattca ggcacagtaa acgccggtga
      181 gcgcagaaat gactctccca tcagtacaaa cgcaacatat ttgccacgca gcatccagac
      241 atcacgaaac gaatccatct ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac
      301 atagctaata atctggcggt tttgctggcg gagcggtttc ttcattactg gcttcactaa
      361 acgcatatta aaaatcagaa aaactgtagt ttagccgatt tagcccctgt acgtcccgct
      421 ttgcgtgtat ttcataacac cgtttccaga atagtctccg aagcggggtc tggctggtgg
      481 tctatagtta gagagttttt tgaccaaaac agcggccctt tcagtaataa attaaggaga
      541 cgagttcaat gtcgcaacaa aacgaaaaga acccacatca gcaccagtca ccactacacg
      601 attccagcga agcgaaaccg gggatggact cactggcacc tgaggacggc tctcatcgtc
      661 cagcggctga accaacaccg ccaggtgcac aacctaccgc ctcagggagc ctgaaagccc
      721 ctgatacgcg taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt
      781 atgcgctgac cactaatcag ggcgtgcgca tcgccgacga tcaaaactca ctgcgtgccg
      841 gtagccgtgg tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg
      901 accatgagcg catcccggaa cgtattgttc atgcacgtgg atcagccgct cacggttatt
      961 tccagccata taaaagctta agcgatatca ccaaagcgga tttcctctca gatccgaaca
     1021 aaatcacccc agtatttgtt aggttctcta ccgttcaggg tggtgctggc tctgccgata
     1081 ccgtgcgtga tatccgtggc tttgccacca agttctatac tgaagagggt atttttgacc
     1141 tcgttggcaa taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg
     1201 ttcatgcggt aaaaccagaa ccacactggg caattccaca agggcaaagc gcccacgata
     1261 ctttctggga ttatgtttct ctgcaacctg aaactctgca caacgtgatg tgggcgatgt
     1321 cggatcgcgg catcccccgc agttaccgca ccatggaagg ctttggtatt cataccttcc
     1381 gcctgattaa tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa ccactggcag
     1441 gtaaagcctc actcgtttgg gatgaagcac aaaaactaac cggacgtgac ccggacttcc
     1501 accgccgcga gttgtgggaa gccattgaag caggcgattt tccggaatac gaactgggct
     1561 tccagttgat tcctgaagaa gatgaattca agttcgactt cgatcttctc gatccaacta
     1621 aacttatccc ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca
     1681 atccggataa cttctttgcc gaaaacgaac aagcagcatt ccatccaggt catattgttc
     1741 ctggtctgga cttcaccaac gatcccctgt tgcagggacg tttgttctcc tataccgata
     1801 cacaaatcag tcgtcttggt gggccgaatt tccatgagat tccgattaac cgcccaacct
     1861 gcccttacca taatttccag cgtgacggca tgcatcgtat ggggatcgac actaacccgg
     1921 cgaattacga accgaactcg atcaacgata actggccgcg cgaaacaccg ccgggaccga
     1981 aacgcggcgg ttttgaatca tatcaggaac gcgtggaagg aaataaagtt cgcgagcgca
     2041 gtccatcttt tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat
     2101 tcgagcagcg ccatattgtc gatggtttca gttttgagtt aagcaaagtc gttcgtccgt
     2161 atattcgtga gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg
     2221 tggcgaaaaa tctcggtatc gaactgactg acgaccagct gaatatcacc ccacctccgg
     2281 acgtcaacgg tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg
     2341 tgaaaggtcg cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg
     2401 ccattctcaa ggcgctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg
     2461 gtgaagtgac tgcggatgac ggtacggtgt tgcctatagc cgctaccttt gccggtgcgc
     2521 cttcgctgac ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca
     2581 acggcgatgc caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg
     2641 cgggagacgc gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga
     2701 ttgtggaagc tgacagcgcc gacggtagtt ttatggatga actgttaacg ctgatggcag
     2761 cacaccgcgt gtggtcacgc attcctaaga ttgacaaaat tccggcgtaa atatggataa
     2821 ggtcacgtcc cctggggagt gaccttacca ccttacttac acatcaagat aactacccaa
     2881 ccgataacca cgctcagcaa ttgcatattt caacgacgct gatgtcagca cttccagctc
     2941 cgttaaccgt gggaaacagt aagcactctg gcgaatcgta ttatcgacaa acgccggatg
     3001 gcacatgact tccagcgagc gttccccgcg atggctggaa tcatcaagca cttgcaggaa
     3061 cagtgcctcg ctaatctctt caccataaaa tgcgctactg aatccgtgcg aactgcgcag
     3121 attagccggt aaatcaccag cgttagatag cggctgacgg tcgatgcgca acgcaatccc
     3181 ctcttcagcc gcaaacctgg caacaatagg gaaaatctgc gggaacatat gcacatgatg
     3241 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
     3301 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
     3361 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat
     3421 agtaaggaca aagttcatcc ctatggccag actcggttca tcacgactca actgcaccgc
     3481 atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca
     3541 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
     3601 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
     3661 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
     3721 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc
     3781 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
     3841 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
     3901 cgtacaggtc atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg
     3961 gatattaacg taatgttctg cttgcttatc gttgtagata gcgttgatca cttcgcacgc
     4021 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
     4081 cgggttttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gcgctcgtgc
     4141 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttttgct tgaagtaata
     4201 aagcaaataa gaacacggca acagattcag agaacgaatt aagccttcgc taaatggcag
     4261 atcgaaaata tttttaacgc cagatgcttt taactgcccg gatgccacac catcaagcaa
     4321 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
     4381 gccgaacaga tcgatagata aatcatcgct gtctttcagc atcagaacat cgcgaataaa
     4441 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
     4501 ataaacggct tcagtgacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
     4561 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
     4621 aaacagaccg cccgcgccgt tggtttcctg accaagataa ccatgactta atggaatacg
     4681 ttcatccagt tcacgcgccg gtaattggcc aacgcgcagt tgggtagtaa cgaaatcagc
     4741 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
     4801 atcaatcatc cgttggcaga gatcgaaaat aatatccagt ttagctttgc caccttcgac
     4861 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
     4921 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
     4981 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc cggacataaa
     5041 taatccagca acaggacaga tatgtgaatt gtcaggtata acgacttact gattaaattc
     5101 agtcaatttc ttacgatagc tcttaggcgt aaaggacgtt aatttcttaa acgttttaat
     5161 aaacaaactc gggctactat aaccggcctc aaacgcaata tccgtcactg aatagttggt
     5221 catttccagt tgttttttgg caaaattaat acggatttca ttaataatct gcattggcgt
     5281 tttgccataa tatcgttgag tcgcccgcgt caaatattcc tgtgatttgg ctgacaacgt
     5341 caccatattc tccagcgccg attcactaaa ctgctcttta tcatgcatct tttctaccgt
     5401 acttttcagc cactgcggta catcatcaat cacctgttct tcgcggtaat gacgtaaacg
     5461 gttaatgaca taaaaagtaa ccatctcaac aaactcttcc agccctgttt cgcggaaatt
     5521 caatgaagaa atcactgttt ccacataggt gagaaaagcg ttattggtcc ggtatacctg
     5581 cgaagctaca aagcaataag gcaacaatgg caggtaatgc tgctcaaaaa agcgtttact
     5641 gatcccaacg ttcaatatgc gcgtggcacc aaactcataa aaactttgat ggtgcgaacc
     5701 taacggaata aaaacaaaat cgccccgttc cagtaacacg cgcttaccgt taatctcctg
     5761 gaaataacgc ccggttaata ccagagtaaa ttcataatag tcgtgctgat gcagtccgct
     5821 gatactctca gttttgttat agataaacac atggaagttt ttgccattaa acaactgctg
     5881 ttctcgggca gtggcaattt ccggcgcgtt aatcactggc tgcatcatcg actccttatg
     5941 ccttcagttt ttcatgaagc tcaattaatt cagtaatcag ttcacgcgca agcatggacg
     6001 tcattaaatg atcctgagcg tggaccagca ccagactcac tttcatctta ccttcgcccg
     6061 catcgccttc aatcagtttc gtctgtacca gatgcgcttc attcaatgcc atccgtgact
     6121 gatccatcat ggcttttgct gcggcaaaat cgccctgctt cgcctgtttc agtgcggcat
     6181 acgccaggct gcgcgcttgt ccggagttga tgataagccc catcaccact tcttccagct
     6241 cttcagcttc cgtttgcgta tcgggaatat tatcgagatc catcatacat cgttcctctc
     6301 ttttcttacc ggcacgatta cccgtaccgg catcgattaa aatttcaggg cgttagcgat
     6361 atcttcttcg ctctcttctt tatcaatcgc attttgtgct ttgttagcca ccacaacaaa
     6421 gggcagataa attaacgttg cgataccaag gttgaagagt gcaaccagca atgcgacgac
     6481 gctaccgttg gtgttaaaga aggctcccag accggttggc atggtccacg gtgcaatatt
     6541 ggtcaccgga ggaataatgc ccatgtagta cgctgccagg gtgattgccg ccagaatcgg
     6601 ttgtaccagt acaaacggga taaacatcac cgggttcatg ataattggca gaccaaacag
     6661 aatcggttcg ttaatctgga agatgccgga cggcagcgcc agttttgcca cctgacgata
     6721 atcagcacgg cgagaggcaa taaagatagc caggatcagg cctaaagtcg caccactgcc
     6781 tccaaggaaa ataaaggagt ccagcatcgg cttggcccag atatggaagg tcttaccggc
     6841 tgccagcgcc gcttcgacgg aaccatattg ctgataggtc gcgatatttt ccagcgccca
     6901 cggcgtcata atgccgttgt ccagtgcggt cagcgccagc gcgccatgaa taccgaagaa
     6961 ccagagcagt ggaacaaaga tcacataggc ccagcccacc acgctaccca acgatgccag
     7021 tggggttgag atggtatcca taatgatctg atggaagttg gtgccccagg tattcaacgc
     7081 ccaggcaata atccccatca cggaaagaat aataaagccg ggaattaatg ccgagaagga
     7141 acgcgatact gaagcaggta cgctgtcggg cagtttaatg acccaattgc ggcggacaat
     7201 aaaggtaaac atttctgcca ccaccaggcc aataataatc ccggagatga tattcgcccc
     7261 acctaaccag tttgcgccaa ccgcataggc ctcaccgaca ctatatgggg tgacggtcat
     7321 aaatgctgca acggataaca acccagccgc cagcgcatcg actttacgct cttctgccag
     7381 cgccatgcca ataaagaacg gtgccattaa agacattatc cctaatgttc cgttatatac
     7441 gttgccgcca atacctttca gaccattaag tgtttcaatg gttgaggcgt cgaggcgaat
     7501 acctaaggaa taaaaaaacg acccctcccc aaagcttaga aaaacgttgt taattaatac
     7561 aaacatggcc cccgcaaggg ttaacggcat taagcgaata aagccatttt tgattgcatt
     7621 aacgtgtggc tgctttccta ttttaactgc aaaagggagg agtacctttt caagcgatgc
     7681 aataacatta ctcatagaaa aataccctta aaaaccgcaa tttaaatatt gcggtattga
     7741 tttatgaaat aactctttga cgggaaaatt taaaataaat taatttgctg cggctttttt
     7801 aatcgctgca accgcagcct taagcacgcc taaaccatcg actttgccat aaagcagcga
     7861 gtcaattact tcaaccggtt tgttgggtaa caaacgctgg atttcgggca acatataagc
     7921 aatctgcggc cctaataaca cgacatcggc attctgacct ttttcaccag ccagtgtttc
     7981 cggaaatgct tcaataatga ccggaacttc atatttttct gcctgtgcgc gcatttttga
     8041 taccagtaaa gaggtagaca tgcccgcaga acaaaacaga taaatgtgtt tcttttccat
     8101 aaaactgccc tcatcgacga ttatctgtca gccagacact ccgcaagcct taacctgctt
     8161 ccatgctctg ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa aggagtatac
     8221 ggtatagacc tgagcaatag tatttctttg accttataaa ttgtctctcc ttgacctgcg
     8281 gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa gataattaag aactcaggcc
     8341 aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca agctttcaga cgaagtgatt
     8401 acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg gagttgatga
     8461 catgtccggt atcatctaac gcgacaaagt aggtttctgc tttaccatca cgttgaccca
     8521 ggatgtaggt ctggcaagta ccacgagcat ggatcatgct cacttcagac gaaggtttac
     8581 ccgcaatttg tgcaacctgc gcccggctca tgcctttttt gacgtctttc accacaggct
     8641 gtacaaactg gtctttggta cgatcataag ccgtacaacc cgccagcatg gttaataccg
     8701 ccgctgcact cagaattcct gccatattct tgttcatatt ccgtcctatt gtttatcagc
     8761 gtgttagata agcctggaat acattgggcg ctttttcaag cccatgaacg aaacggctcc
     8821 gctttcagag gattcctgta tgacgtttta accaccattc agcccgctgt cgcttgtcgt
     8881 ttcagtagca acgggttagc tttaaggaag ttttgtcttt tctgtctgga ggggttcaat
     8941 gacattgcaa caacaaataa taaaggcgct gggcgcaaaa ccgcagatta atgctgaaga
     9001 ggaaattcgt cgtagtgtcg attttctgaa aagctacctg caaacttatc cgttcattaa
     9061 atcactggtg ctcgggatca gcggcggtca ggactccacg cttgccggta agctgtgcca
     9121 gatggcgatt aatgagctgc gccaggaaac cggcaacgaa tcactgcaat ttattgccgt
     9181 acgcctgccc tatggtgttc aggccgacga acaagattgc caggatgcca ttgcctttat
     9241 tcaaccggat cgcgtattaa ccgttaatat caagggcgcg gtattggcta gcgagcaggc
     9301 attgcgggaa gcatgcattg aactgagcga ttttgtccgt ggcaatgaaa aagcgcgtga
     9361 gcggatgaaa gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg tgggcaccga
     9421 tcatgcagca gaagccatta ccggattctt cactaaatat ggtgacggcg gtacggacat
     9481 taatccactg tatcgtctca acaaacgtca gggtaaacag ttactggcag cattaggttg
     9541 cccggaacac ctttataaga aagcgccaac ggccgatctg gaagatgatc gcccttctct
     9601 gccagatgaa gtggcactcg gcgtgaccta tgacaatatc gacgactatc tggaagggaa
     9661 aaacgtacct caacaggtcg ccagaacaat agagaactgg tatctgaaaa ccgaacataa
     9721 acgccgtccg ccaattaccg ttttcgatga tttctggaaa aagtaataat ttgcacctat
     9781 tggattgtgc aaaaaagagt aatttgttca cgccggatgc ggcgtgaacg ccttattcga
     9841 cctacaaaac tatgcaaatt caatacattg caggagtcga ataggcctga caggcgtagc
     9901 acgtcagacg gtgtaacctt tgtcatcgac ccgcctcttt tttaatcgct tcccggctgt
     9961 tacactggat agataaccag cattcggagt caacagtggt acggcgttta acttctccgc
    10021 ggctcgaatt tgaagctgcg gcaatttatg aatatcccga acatttacgt tcattcctta
    10081 atgacttacc cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac accatgccgc
    10141 tctatatcgg caaaagcgtt aatatccgca gtcgggtgct ttctcattta cgcaccccgg
    10201 atgaagccgc catgctacgg caatcccggc gaattagctg gatctgcacc gcaggcgaaa
    10261 tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca acagccgctg tttaataaac
    10321 ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa tgaaaagcgc gtcgatgtgg
    10381 tgtatgccaa agaggtggat ttttcacgag cccccaacct gtttggcctg tttgccaata
    10441 ggcgcgcagc tttgcaagca ttgcagagca tcgctgatga acaaaaactt tgttatggtc
    10501 tgttgggact ggaaccgtta agtcgcggtc gtgcgtgttt tcgttcagcg ctaaaacgtt
    10561 gcgccggagc atgctgcggt aaagagagcc atgaggaaca tgcgctacgc ttgcgccagt
    10621 ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg ggcggtggcg ctgaaagaac
    10681 agcacccgga aatgactcaa tatcatatta ttcaaaactg gctgtggctg ggggcggtta
    10741 attcgctgga agaagcgaca acgttaattc ggacacccgc cgggtttgat cacgacggtt
    10801 ataaaattct ttgtaagccg ctgctttccg gtaactatga aattactgaa cttgatccgg
    10861 cgaatgacca gcgagccagt tgatttcact gaacaacagt ttaccttgcg gttgtaaaag
    10921 ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg tcggtagtga gcagtttgtc
    10981 gcccagttgc cacgccccgt taatcacaaa caccaccccg ccgcgtgagc caaaggtagt
    11041 aaacgtacgt tcggcaattc tgactttcgc cttacagaca tccaggcgcg tcataatatt
    11101 gaaatccatc gacatctgcc ctgccgtcag tttcgctttt accacctggt ccgccgcaaa
    11161 ggcaaaaggc tgtaacggtt ttaaggtatg gttaaagtgg tctgcgcttt caaggaacat
    11221 ctcgccgcct tccagcaacg tcactatcct ttccatacca ggaaacaaag aaaactcccc
    11281 attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa tcacgttttg ccggtgggaa
    11341 agtgcaaatt tcgcgcgttt caccggcagc gtttcgccac aaattcaccg acattttacg
    11401 catatcaaag tattccatac tcgctcccgg cccgttcttt tcattgttat cgttcaggta
    11461 accgtggatg gttatcggca actcgggtga tttacgttag tggtgatcag gcttttttta
    11521 caactgtttg agaagagaaa agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta
    11581 gtggacaaga ccggcggtct taagtttttt ggctgaaaga ttattcagca gttgcaggca
    11641 ttttaccttt tgccgctgga cgttctgtca gacgcttctc aaaattagca ttaaattgct
    11701 ttttctgttc cggcgtcagg atgttgtaaa ttttgttctg ggtttccatg tgcgccagca
    11761 tgttagcttt gcgctgttct tccatttttg cgatctgcgc ttcagctttt gctttatcga
    11821 aggtatcgct ggcaatgatg tcatgcattg cgcgacgttc ttccagcggc ggacgtttca
    11881 tctggtcacg ctggcctttc atgatttcgc gaatctgctg tttctgcgcg tcggtcaggt
    11941 tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt gcctttgtgg tgcatcatcg
    12001 gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc atgggccagg ttagccgcgc
    12061 caagagccag ggtagaggca acaaacagtg cagttaattt acgcatattc tatatccttc
    12121 ctttcagtta tttattacgg ctttcttaag tagcgtgccg tgttgacgag attaacttta
    12181 ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt
    12241 ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga gtgatgagag aatattacaa
    12301 caccatgatt ttgcagagat tatgaagaac tataccggat gactggtgat aaataaagca
    12361 aataaccagg attaatctgt attaatttat aagaaggtaa cttaataccc gcagaaagat
    12421 ttctgcgggt aagtattagc ttattttttc gagcattaat cccgcgcgta atcccagcgc
    12481 taccaacgga ttagggaata acacatactc tacatcatgg gttacggtaa aacgttcctc
    12541 accgtcctgc gccagcaacg ttcctttctt aaacggcata aaattcagcg tgtcacttgc
    12601 catatgcatt tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc gataacggag
    12661 cggcggtgtt ctcacgatac cgacactctc accagatagc agcgcagcga ttgcgctggc
    12721 agttactgca aactggcgaa gatcgttttg cccaaagggc aacgctttgc caagttccag
    12781 cgtacaggcc agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac caccaggctc
    12841 ctgatggaac accagcgcct ccagccccgc cgcacccagc cacgtcagga atttctcgtc
    12901 ccagggaatg tcgcgttgcg gtaatacacc gaactgcgga tgcaaggagc cacgaattgc
    12961 ggtatgtaga tcaaggtgcc agcgcacaga ttctttgccc tggtcataaa aatcttccag
    13021 gcactgttcc agttcgcgcg cccgacaggt ttccccgctt tcagcaaaca gctgccaacg
    13081 accgccaaac attcggttca tatcgctatg gcaataacgt ttcccttgct tcagcgcagg
    13141 aggattcccg aggatcacca gcaaccgcca ccgtaacggg atctcgccgt gagatatcgc
    13201 gccaagcaat gcatcaagca tctccacagg tgccgtctca ttaccgtgga tccccgcaga
    13261 aatcaccaga gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac catcgcccag
    13321 ccagcgccag cgaacgccgt tgatttctcg ctcggtgatg acaggttttt tactcgttaa
    13381 ggtcagagca agaaaattat ccattgcccc ctccctcgcg ctggaacgga taaaccgaac
    13441 cgagattcag taattggctc aatacatcca gtgcttcccg cccttcgcgc agcaattgcg
    13501 ggtcggccag atcggcagca gtaagacgat cgcggtagta acgatccacc cagtcattga
    13561 gcgcattaaa caaggtatcg ttcatcatca ccgccggatt caccgcccgg cgttcttctt
    13621 gtgtcaatac cacccgcaac cgcaggcacg ccgggccacc gccattcgcc atgctttcac
    13681 gtaaatcaaa gacttttagt tcgctaatcg ggttgtcagc ggcaaggagt tcattgagat
    13741 aaccccatac tccggcgtgt tcccgacact cctgaggcag caccaacatc atggaaccat
    13801 catcgcggct caacagttgg ctgttaaaca gatatgtaga caccgcatcc gacacggaaa
    13861 cctgagttgc cggaacttct atcgccataa aaccgttcac ccgcgcacgc aggtttgcca
    13921 gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg cggttactca
    13981 cggcaatcac gtcattatga aaaacgccct ggtcgataac gtccgggttt tgctgggcga
    14041 aaatcacctg ttggggattc acctgattca gccttgccac cgcctcgctg gcttcgcgag
    14101 tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ctcttctcgc ccgtagacaa
    14161 aaagttgcat acccggttca ccgtaatgac cgccgagacg attgtggttt gccgccccct
    14221 catcaccgag caaggctacc tgcggcaacg ccgaatggac actaaatttc tcttcgtcgt
    14281 taaaaatcgc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg tgaaatttat
    14341 tgttcaggtt agcaacggtg agatgcactt tgccatccag cgtatcggca gatggcgcga
    14401 tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa agccagtgcg
    14461 gtgcctggcg tacaaccttt tccagtacct gctcatcgct accactgaat cccaactgac
    14521 gcagcaccgg aataaacgga cgctcgtgag gcgggatcac ggcctggggg aatcccgcat
    14581 cggcaagggt tttcattttc agtaagccct gttttgccgc cagtcgcggg ttagagacct
    14641 gaaaacggtg acgggtagag gctttattac caaacgacag gcccgcgtaa tgatgcgtca
    14701 gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct catcggaaaa
    14761 atccagcccg gggttaagcg ttgcgggcaa agttaacgag tccgactcca ggctcgccat
    14821 aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac cggatgcacc
    14881 aatgccgccg aatggcgcgg tactggcagc accagtaagc ggtttgttcc agttaacaat
    14941 ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg aaaccagacc
    15001 gcaagagagt ccgaagcgag tgttattcgc cattcgaatc gcttcctcga aagaatcata
    15061 acgccagacg cgcaataacg gtccgaacac ctcttcatct ggtacgccag caacgcctgt
    15121 catttcaatg atccccggcg tcagcaacga tgtccctgct tgtaataagc gcggcgcaag
    15181 cagggttcgt ccacccatcg cttccagttg ctgccaggca gtaaacacct gctgtgcggc
    15241 ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt tgcccggcgt
    15301 taatcgctgg ctgacggcaa ccagacgagc aagaaatgca tcgccctgcg ccccgctttt
    15361 cagcaataca cggcgggcgc aggtgcagcg ttgcccggct gtaacaaacg ccgactgaat
    15421 ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataatcagcg ggttattgcc
    15481 gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat gcagctggta
    15541 gccggtattg gcgctgccgg taaacagcaa gccgtcgaga tcctccagcg cactcagcgc
    15601 ctgacccgtt tcacacccgc cctgcaccag gttcagcacg cctggcggaa agccagcctg
    15661 ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg gtttaaagat
    15721 aatggtgtta cctgccagca atgcgggaac gatatgtccg ttcggcaaat gaccagggaa
    15781 attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc tcgccgcacc
    15841 gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa ttgatatcgc
    15901 gattttattg atcatcgccg tcaattcggt tgccgcctcc cagcgcggct tgccggtttc
    15961 tcgggcaatg attgcggtta attcgccttt attcctttcc agcaatccgg caaagcgttc
    16021 gacaacggcc tgacgttcag caaatgagag ccgcgcccag cgcggaaacg ccgcacgggc
    16081 tgcccgacaa gcctgcccga cctgagcggc atcggcatca ttgccttgcc ataacacctc
    16141 gcccgatacc ggattacgct tcacacgcga tgcaccctgg cccgttatcc agtcaccgtt
    16201 aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg aacgcgatcc
    16261 cctgcatggc atttgagggc atccagttgt gcggcggtta aaatcaaacg ctcggttgcc
    16321 ggatcggtac gcaccagcac cacgcggaaa tggtgataat tttcattggc gaccaggcag
    16381 gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg acttttacgg
    16441 atggcgcgca cgcggtcgat gtcacactca agcgtcggcc caccgtcgaa gatgtcgata
    16501 tagttgcggt agcgaaaacc ttctttctcc agcaccgcgc gggcaggcgc ggtttgcgga
    16561 tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgcgtata gatcggatgt
    16621 ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa atcggcgcgg
    16681 ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga atagccgtgt
    16741 tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc gcgaaaagcc
    16801 gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt gcgccagtcc
    16861 gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact gagaaacaac
    16921 gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt gccgacgcga
    16981 tagttgtacc agggatcgtt cagcccaact gccacctcaa tggcgcaaat ccccgccacg
    17041 gtgcctgtct cgctatcttc cagtacaaac acataaccct gctcactttt gggcagttca
    17101 ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc attggcggga
    17161 agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga gacatctgag
    17221 cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt tcgcaagcaa
    17281 ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc gcaggcgcaa
    17341 aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc gcttcctgag
    17401 agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc agcaaaccta
    17461 agccgcgaac ttcattgaac aaaccatagc ggtgattaat aatattaaga cgctcaacaa
    17521 accagtcgtg acgttgctta accccgttaa gcatctctgg cgtattgatg agctccagca
    17581 ctttgcctgc caccgccgag gccagcggat tgccgccata ggtggtgcca tgagtgccaa
    17641 cggtcatcac gctggcgcac tcttcggttg ctaacaacgc accgaccggg aaaccgccgc
    17701 ccagagcttt ggcggtagtt aacagatcag gcgtcacgcc gtagtgcata taggcataca
    17761 gttccccggt acgcccgacg ccggtttgta cttcatcaaa aatcaacagc gcattgtggc
    17821 gatcacacaa ttcacgcaga ccttgtaaaa atgcgttgct ggctggcacc acaccgcctt
    17881 caccctggat gggttcgaca atcaccgcac aggtagagtc gtcaatcagc gcgctggcag
    17941 agttaatatc gttatatgca gcatgacgga tatctggtgg cagtggcgca aaatcctgtg
    18001 aataggctgg ctgcccaccc gcactaacag taaacagcgt gcgaccatga aacgcatttt
    18061 taaacgccac gataccgctc ttatggctgc cgtagcggtc gtgagcgaat ttacgcgcca
    18121 gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaaaacg cgatcggcaa
    18181 acgtggcgtc gatcaatttt ttcgccagtc gcagtaccga ctcgttggtg taaccgttgc
    18241 cggtatgcca gaacttactc gcctgttcgt tcagcgcctc acgcagttcc ggatgcgcat
    18301 ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc ccctgctgat
    18361 cccacaagcg cgaaccttcg ccacgtaccg gtataaacgg tgccggagcg taaacaggta
    18421 tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc tctacagtaa
    18481 atagctcgtt atttatatgt taataataag taatgttttc gctgtaaatg tagattgcag
    18541 ggttcgtgcc agccagtgat gaaagtgcat aaacggcgga ggctaactgg aaatcaagga
    18601 gttataacca aaccatatgc atttaaagtg catattaagt gaatacgttt gcgatgtggg
    18661 tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt ctggaattgt
    18721 gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc gcaccaaaag
    18781 cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat cacttaacaa
    18841 caggcggtaa gcaaagcgaa attctgctac catccacgca ctctttatct gaataaatgg
    18901 cagcgactat gaaatttgtc tcttttaata tcaatggcct gcgcgccaga ccccaccagc
    18961 ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag acaaaagttc
    19021 atgacgatat gtttcccctc gaagaggtgg cgaagctcgg ctacaacgtg ttttatcacg
    19081 ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt gccgtgcgtc
    19141 gcggctttcc cggtgacgaa gaagaggcgc agcggcggat tattatggcg gaaatcccct
    19201 cgccgctggg caatgtcacc gtgatcaacg gttacttccc gcagggtgaa agccgcgacc
    19261 atccgataaa attcccggca aaggcgcagt tttatcagaa tctgcaaaac tacctggaaa
    19321 ccgaactcaa gcgtgaaaat ccggtgctga ttatgggcga tatgaatatc agtcctggcg
    19381 acctggatat tggcattggc gaagagaacc gtaagcgctg gctgcgtacc ggaaaatgtt
    19441 ctttccttcc ggaggaacgc gaatggatgg agagactgat gagctgggga ttggttgata
    19501 ccttccgcca tgcgaatccg caaacagcag atcgtttctc atggtttgat taccgctcaa
    19561 aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc caaccgctgg
    19621 cagaatgttg cgtagaaacc ggcatcgact atgaaatccg cagcatggaa aaaccgtccg
    19681 atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct ggctcaaact
    19741 gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat ttgttgcctg
    19801 ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc tattatcaag
    19861 tttgatatag gaaactccac gatgaacgct gagcgtaaat ttctttttgc ctgtcttatt
    19921 tttgcgctgg ccatttacgc tatccacgct ttcggtttat tcgatctgct caccgattta
    19981 ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct ctatattctg
    20041 ttattcatca ttgccaccct ctttctgtta ccaggaagca tattggtgat cgcgggagga
    20101 atagtttttg gcccgttctt agggacacta ctctcattaa ttgccgccac gctggcctcc
    20161 tcgtgctcat tcctgctggc gcgctggatg ggacgtgatt tactgctgaa atacgttggc
    20221 catagccata ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat tgattttctt
    20281 attctgaccc gcttaatccc gttgtttcct tacaatattc aaaattacgc ttacggatta
    20341 accacaatcg ccttctggcc ttataccctt atttcggcac tcacgaccct gcccggtatt
    20401 gttatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct gcgctttatt
    20461 ttacaactct gtctggcggg cctggcgctg tttattctcg tccagctcgc aaaactctac
    20521 gcccgacaca aacatgtgga tctgtctgct tcgcgccgca acccacttac tcacccaaaa
    20581 aatgaaggat agaacgatgt tgcaacatta ttcagtgtca tggaaaaaag gactgactgc
    20641 actctgctta ctggctgttg ctgggcttag cggctgcgat caaaaagaga atgcggcggc
    20701 aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg ccaataacca
    20761 tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca ctcgtcacgg
    20821 tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatgggtt atgcgacccc
    20881 gaaagcattt tatgaagccc tgaaagaggc aggtggtaca ccgggcgaaa acatgacgat
    20941 ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg tcaactggca
    21001 aggggcggca aaaacgtatt ccttcgatga agtgattgtt gacagtaatg gcaagaaact
    21061 ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg gttgcctggt
    21121 gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt atggtgcggt
    21181 tgaaaaacga ggtgaagtta aattcaaagg caatgcctcg gttctcccgg cgaataacac
    21241 gctggcaacg gttaccttta aaatcaccga ataaagccag gataaaggat gatgaagatg
    21301 caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt cgctatgctg
    21361 ctggcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt tgcggcgttt
    21421 gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc actggcggca
    21481 gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc tgcgtttttg
    21541 atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggctcgct gtcgtggacc
    21601 agttcgatgg ccggcgcggc actgtgcttt tttatcgcca gagtgatggg ccgcgaagtg
    21661 gtggaaaaat taaccggcaa aaccgtactt gacagtatgg acggcttttt cactcgctac
    21721 ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg tccctttcga tccaatcagc
    21781 tatgctgccg gtttgacttc aatacgtttt cgctcgtttt ttatcgccac cgggcttggt
    21841 cagttaccgg cgactattgt ttattcctgg gcgggcagca tgttaacagg cggtaccttc
    21901 tggtttgtca ccggactgtt tattctgttt gccctgaccg tggtgatttt tatggcgaag
    21961 aaaatatggc ttgagcgcca gaagaggaat gcctgatggg tttaccgccg cttagcaaaa
    22021 ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc gaggtgctaa
    22081 gccccattcg ctggtggggg cggatcccgt ttatctttta tctggtgtcg atgtttgttg
    22141 gctggctgga gcgcaaacgc tcaccgctcg atccggtagt acgatcgctt gtcagcgcgc
    22201 gcattgcgca aatgtgcctg tgtgagtttt gtgtggatat caccagtatg aaagtcgccg
    22261 agcgcaccgg cagcagcgat aaactgctgg cagtggctga ctggcggcaa agcccgctct
    22321 ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgta acgccgccaa
    22381 cggtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag gagctcaccg
    22441 aactgacggc attgatcggc ctgcaaaatc tgtcagcccg ttttaattct gccatggaca
    22501 ttcccgctca ggggctgtgc cgtattccgg aaaaacgttc ttaaggagag atgatgcgcc
    22561 attgtgggtg gttgctggga ttgttatcgc tgttttctct ggcaacacat gccagtgact
    22621 ggcaagaaat taaaaatgag gccaaagggc aaactgtctg gtttaacgcc tggggcggcg
    22681 ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc cattacgcta
    22741 taaacctgaa gattgttcgt ctggcggatg ccgcagacgc ggtgaagcgc attcagaccg
    22801 aagctgctgc cggacgtaaa acgggcggct cggtggatct gctctgggtg aacggcgaaa
    22861 acttccgcac cttaaaagag gccaatttac tgcaaaccga ctgggcagag actctgccca
    22921 actggcgcta tgtcgacaca cagctgccgg tgcgggaaga tttttcagtg cctacagaag
    22981 gggctgaatc gccctggggg ggcgcacaac tgacatttat cgcccgccgc gatgttacgc
    23041 cacagccacc acaaacgccg caagccttac tggagtttgc taaagccaat cccggcacgg
    23101 ttacctatcc gcgcccaccg gactttaccg gcacggcgtt tcttgaacag ttgctgatta
    23161 tgctgacgcc cgatcccgcc gcattaaatg aagcgccgaa cgatgcgact ttcgcccgtg
    23221 tcactgctcc cttgtggcaa tatcttgatg cgctgcatcc gtatttgtgg cgcgaaggaa
    23281 aggatttccc gccttcgccc gcgcggatgg atgctctgct gaaagccgga acatttcgcc
    23341 tgtcgctgac ctttaacccc gcgcatgcgc agcaaaaaat cgccagcggc gatttgcctg
    23401 caagcagtta cagttttggc tttcgcgagg ggatgattgg caacgtgcat ttcgtcacca
    23461 ttcctgccaa cgcgaatgcc agtgctgcgg cgaaggtagt tgccaatttc ttgctctcac
    23521 ccgatgcgca attgcgtaaa gcagatcccg ctgtctgggg cgatccttct gttctcgatc
    23581 cgcaaaaact gcctgatggg cagcgcgaaa cattgcaatc aagaatgccg caagatctgc
    23641 cgccggtact ggctgaaccg cacgcaggtt gggtaaatgc gctggaacaa gaatggctac
    23701 accgttacgg tacgcattaa tctttttgct gtgggcgatg gtggcggtga tttatgcacc
    23761 gctgatcccg gcagctctca cgctgatatc gcctgctttg tcgttgacac actggcaggc
    23821 gttatttgcc gatccacagt taccgcaggc attactggca acgctggtgt cgacaaccat
    23881 cgcggcggtc ggggcgttgt tgattgccct actggtgatt gtggcgctgt ggcctgggcc
    23941 gaaatggcag cgtatgtgca cccgtctgcc gtggctgctc gccattcccc atgtggcttt
    24001 tgccaccagc gcccttctgc tctttgctga cggagggctg ctttatgact atttcccgta
    24061 tttcactccg ccaatggaca aattgggtat tgggctgggc ttcacgctgg cggtgaaaga
    24121 aagcgcattt ctgctgtgga tcttagcagc ggtattgagt gaaaaacggc ttttacagca
    24181 agtcatcgtg ctggattcgc tgggctacag ccgctggcaa tgcctgaact ggctgctgtt
    24241 gccctccgtc gcgcctgcgc tggcaatggc gatgctggcg attgttgcct ggtcgctgtc
    24301 ggtcgtagat gtggcgatta ttcttgggcc aggtaatcca ccgacgctgg cagtaattag
    24361 ctggcagtgg ttaactcagg gcgacgccga tcaacaaacg aaaggcgcac tcgccagcct
    24421 gctgctgatg ttgttactcg ccgcctacgt tttgctgggc tatctgctat ggtgtacctg
    24481 gcggcgtact attccccgcg tagatggcgt tcgtaagcct gccacgcctt tattgccagg
    24541 cattacgctg gcgagttttt tacccttgac cagtgtgctg tgtgtggttc tgctggcgat
    24601 cctcgcggat cagtcgacga tcaatagtga agcgctcatc aacagcctga caatggggct
    24661 ggtggcgaca ttcatcgctt tgctcctgtt actgctgtgg ctggaatggg ggccacagcg
    24721 tcgccagttg tggctatggt tgccgatttt attaccggca ctgccgctgg tggcgggcca
    24781 gtacacgctg gcgctatggc tgaacatgga tggaagctgg acggcggtgg tctgggggca
    24841 tctgctgtgg gtaatgccgt ggatgctgtt tatcctgcaa cccgcctggc agcgcattga
    24901 ttcacggtta attttgattg cacaaacact gggctggtcg cgggccaaaa tcttctttta
    24961 cgtgaaatgt ccgcttatgc tacgccctac gctgattgcc ttcgcggtgg gattttcagt
    25021 cagtattgcg cagtatatgc cgacgctgtg gctgggcgcg gggcgttttc cgacgctcac
    25081 cactgaggcg gtggcattaa gcagcggcgg cagcaacggt attctcgccg ccctggcttt
    25141 atggcaactg ctattaccgc ttattatttt tgccctgacc gcgttagtcg caaaatgggt
    25201 aggttatgtc agacaaggac tccgctaatg ctctgcgtga aaaatgtttc gctacgttta
    25261 ccagaaagtt gcttgctgac aaacgttaac tttacggtgg ataaaggtga cattgtcacg
    25321 ttaatggggc cgtctggctg tggaaaatcc actctgtttt catggatgat tggtgcactg
    25381 gccggacagt tttcttgtac aggtgagcta tggctcaatg agcaacggat tgacatgcta
    25441 cccaccgcac agcgtcagat tggcattctt tttcaggatg cactgttatt tgaccagttc
    25501 agtgtcgggc aaaatttact gctggcgcta ccggcgacac ttaaggggaa tgcccgacgt
    25561 aatgccgtga atgatgcact tgagcgttca ggccttgacg gagctttcca tcaggatcct
    25621 gctactttgt ctggcggtca gcgagcgcgc gttgctctgc ttcgcgccct tctcgctcag
    25681 ccaaaagcgt tgctactgga tgagccattc agccgtcttg atgtggctct gcgcgataat
    25741 tttcgccagt gggtgttcag cgaagttcgc gaactggcga tccccgtcgt tcaggtaacg
    25801 cacgatctcc aggatgttcc tgctgatagt tctgttctgg atatggcgca gtggtcagaa
    25861 aattacaaca aactgcgata acgcaaagtt tttctcaatg cgtcagttca gaatggcgca
    25921 ctcaaaacta caatgtcggg attttcgatg aaacgtgttt ctcaaatgac cgcgctggca
    25981 atggctttag ggctggcttg cgcttcttcg tgggccgctg aactggcgaa gcctcttaca
    26041 cttgaccagc ttcaacaaca aaatggcaaa gcgatagata ctcgctccag cgcgttttat
    26101 aacggctggc cacaaacctt aaatggccct tctggtcatg aacctgccgc cttaaacctc
    26161 tctgctagct ggcttgacaa aatgagcacc gaacagctca acgcgtggat caagcaacat
    26221 aacctgaaag ccgatgcccc ggtggcgctg tacggtaatg gcaaagatgt cgacgccgtc
    26281 aaaacgcgac tgcaaaaagc aggttttacg catatctcca ttctgagtga cgcgctaagc
    26341 gaaccttccc gtctacaaaa actgccgcac tttgagcagc tggtttatcc gcaatggctg
    26401 cacgacctgc aacaaggtaa agaggttacg gcgaaacctg ccggtgactg gaaagtcatt
    26461 gaagcggcca ggggcgctcc taagctttac cttatcagcc atattcccgg tgctgactac
    26521 atcgatacca acgaagtgga aagcgaaccg ctgtggaaca aagtttctga tgaacaacta
    26581 aaagcgatgc tggcaaaaca cggcattcgc catgacacca cggtcattct gtacgggcgt
    26641 gacgtatacg ctgcagcgcg tgtggcgcag attatgcttt atgccggcgt gaaagatgtg
    26701 cgcctgctgg atggcggctg gcaaacctgg tccgacgcgg gtctgcccgt tgagcgcggt
    26761 acgccaccga aagtgaaagc ggaaccggat ttcggcgtga agatcccggc acaaccgcag
    26821 ttgatgctcg atatggaaca ggcccgtgga ctgctgcatc gccaggatgc atcgctggtg
    26881 agcattcgtt cgtggccaga atttatcggt acgaccagcg gttacagcta tattaaacca
    26941 aaaggtgaaa tagccggagc acgttgggga cacgctggta gcgactcgac gcatatggaa
    27001 gatttccata atccggatgg caccatgcgt agcgccgatg atattaccgc tatgtggaaa
    27061 gcatggaata tcaaaccaga tcagcaagtt tcattctact gcggcaccgg ctggcgcgcg
    27121 tccgaaacct ttatgtacgc acgagcaatg ggctggaata acgtctccgt ttacgacggc
    27181 ggctggtacg aatggagcag cgatccaaaa aatccggtag caaccggtga acgcggcccg
    27241 gacagcagca aataacattg caattactga cgctggagcg acttcagcgt cagataccca
    27301 ctccacaccc gcgtaaaggt cgtcatccag cacaacgcac cgaagatcca cgcaaaccac
    27361 gcaaaccatg cagggaataa acatcccagc acaaacagta agatcgtttc tgtgccttcg
    27421 gttaatcccc ccagataata aaacgattta tgcgcataac cgggattatc aatctgatat
    27481 ttcgccgcca gcgcagcaaa agcgagaaaa ctgctacccg tgccgataaa cgcaaacagc
    27541 agccagccgc ctgccagggc gttttgctgc ggtgcggcga ggataaaccc aaaaggtacc
    27601 agcgcgtaaa aaagaaaatc gagagaaata tcaagaaagc cgcccgcgtc ggtaagccct
    27661 ctgcgccgcg ccagcgcacc gtccagacca tcaagcagcc tgttcaacaa aatgacgaca
    27721 agcgccgcca gataccagcc cagcgccaga aaaggcagtg ccagcacacc gatggcaaat
    27781 cccactaatg ttaaaccatc tggcgtaatg cccggtttat caagaacccg cacacactga
    27841 tgcaacaacg gtttaatccg gggatgaaga tggcggtcta gcacaggcac tccttaaata
    27901 taaagccttt ctgattgagc aacagtgcgg atattatggc atttttcgct tatctgcccg
    27961 tgtgtaattt atgaaaatga ttgaagttgt tgccgccatc attgaacgtg atggcaaaat
    28021 tttactcgcg caacgccccg cccagagcga tcaggcggga ttatgggagt ttgccggtgg
    28081 taaagtcgag ccggatgaaa gccagcagca ggcgctggtg cgtgagttaa acgaagaatt
    28141 gggcatcgaa gcaactgtgg gtgagtatgt tgccagccat cagcgagaag tttcgggccg
    28201 gattatccat cttcatgcct ggcacgtacc cgacttccac gggacgttac aggcacatga
    28261 acatcaggcg ctggtctggt gctcacctga agaggcgctg caatatccgc tggctcctgc
    28321 tgacattcca ttattagagg cgtttatggc tttacgcgcc gccagaccag cggattagtg
    28381 ctaagggttt tgtcatcccg ctggcattgc agcagtattc cttcggcttt aattaccgcc
    28441 ccttcagaat aatttttatc ctgataaacg cagcactgag tacagggctg cgctgactgc
    28501 ccgcctgaac tgaccacttc tggcggtacg tttacctcca cgtccggacc ataatgcggg
    28561 ttagccagtg caattaatgg aaatgctaat actacggcga acaatgctcg actcacaggg
    28621 aactccttaa cgttattgtc tctgctactg ataacggtag ccgggtggca aaactttagc
    28681 gtctgagtta tcgcatttgg ttatgagatt actctcgtta ttaatttgct ttcctgggtc
    28741 atttttttct tgcttaccgt cacattcttg atggtatagt cgaaaactgc aaaagcacat
    28801 gacataaaca acataagcac aatcgtatta atatataagg gttttatatc tatggatcag
    28861 acatattctc tggagtcatt cctcaaccat gtccaaaagc gcgacccgaa tcaaaccgag
    28921 ttcgcgcaag ccgttcgtga agtaatgacc acactctggc cttttcttga acaaaatcca
    28981 aaatatcgcc agatgtcatt actggagcgt ctggttgaac cggagcgcgt gatccagttt
    29041 cgcgtggtat gggttgatga tcgcaatcag gtacaggtca accgtgcatg gcgtgtgcag
    29101 ttcagctctg ccatcggccc gtacaaaggc ggtatgcgct tccatccgtc agttaacctt
    29161 tccattctta aattcctcgg cttcgaacaa accttcaaaa atgccctgac tactctgccg
    29221 atgggcggtg gtaaaggcgg cagcgatttc gacccgaaag ggaaaagcga aggcgaagtg
    29281 atgcgtttct gccaggcgct gatgactgaa ctgtatcgcc acctgggcgc ggataccgac
    29341 gttccggcag gtgatatcgg ggttggtggt cgtgaagtcg gctttatggc ggggatgatg
    29401 aaaaagctct ccaacaatac cgcctgcgtc ttcaccggta agggcctttc atttggcggc
    29461 agtcttattc gcccggaagc taccggctac ggtctcgttt atttcacaga agcaatgcta
    29521 aaacgccacg gtatgggttt tgaaggcatg cgcgtttccg tttctggctc cggcaacgtc
    29581 gcccagtacg ctatcgaaaa agcgatggaa tttggtgctc gtgtgatcac tgcgtcagac
    29641 tccagcggca ctgtagttga tgaaagcgga ttcacgaaag agaaactggc acgtcttatc
    29701 gaaatcaaat ccagccgcga tggtcgagtg gcagattacg ccaaagaatt tggtctggtc
    29761 tatctcgaag gccaacagcc gtggtctgta ccggttgata tcgccctgcc ttgcgctacc
    29821 cagaacgaac tggatgttga cgccgcgcat cagcttatcg ctaatggcgt taaagccgtc
    29881 gccgaagggg caaatatgcc gaccaccatc gaagcgactg aactgttcca gcaggcaggc
    29941 gtactatttg caccgggtaa agcggctaat gctggtggcg tcgctacatc gggcctggaa
    30001 atggcacaaa acgctgcgcg cctgggctgg aaagccgaga aagttgacgc acgtttgcat
    30061 cacatcatgc tggatatcca ccatgcctgt gttgagcatg gtggtgaagg tgagcaaacc
    30121 aactacgtgc agggcgcgaa cattgccggt tttgtgaagg ttgccgatgc gatgctggcg
    30181 cagggtgtga tttaagttgt aaatgcctga tggcgctacg cttatcaggc ctacaaatgg
    30241 gcacaattca ttgcagttac gctctaatgt aggccgggca agcgcagcgc ccccggcaaa
    30301 atttcaggcg tttatgagta tttaacggat gatgctcccc agggaacatt tcttatgggc
    30361 caacggcatt tcttactgta gtgctcccaa aactgcttgt cgtaacgata acaagcttca
    30421 agttcagcat ccgttaactt tctgcgatag cagcagatat gccagtaaag aaatcccatt
    30481 agactatttt tttgataatc ttcttcgctt tcgaacaact cgtgcgcctt tcgagaagca
    30541 agcattatat aatgccaggc cagttcttct tcaattgtcc cgttttgaaa agctgtgctt
    30601 gatatcgaga tcatccatga taattccgcc gcccatatta gcttcgccga ggatttaccg
    30661 gagctatgat tagcgcaatc agagatatag tctgagggaa aaacagcaaa tttattcaac
    30721 aaggcgataa cctgctctgg ggcttcctcc atgtttgctt taaaggtatt ggctccatgg
    30781 tcgccagaaa gaaaatgctc cattaaggca caataacttt cgctatcttc gataccccat
    30841 tgttcctcta aagactcgcg tcttttactt atgatatcga tcgagtcaaa aggaagcaca
    30901 tgatattgga aggtatcttt gccaggttca ggctttcgcg gccagaactc cagcgtttca
    30961 gaccattgct tatgatagaa tcgataaggt gcgatcaatt gtagcgcctg taatttctcg
    31021 atactgagcg gctcaatacc tttagcctga taataatgca gctgttcttt ttttgcttta
    31081 aaaccggccc gaacaataag ccccatcata attaatagat aaagaaaaga gcatcccgcg
    31141 gtaatcaggc ctctttcatt caaaccgttg gatgttatcg ctgcgaatac aaacattaca
    31201 gcgacaacac atgtt
//
LOCUS       DAZKFZ010000037        31127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000037 DAZKFZ010000000
VERSION     DAZKFZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..546
                     /locus_tag="ACHOQW_001542"
     CDS             <1..546
                     /locus_tag="ACHOQW_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP9572635.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLS"
     gene            776..1159
                     /gene="secE"
                     /locus_tag="ACHOQW_001543"
     CDS             776..1159
                     /gene="secE"
                     /locus_tag="ACHOQW_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9572636.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1161..1706
                     /gene="nusG"
                     /locus_tag="ACHOQW_001544"
     CDS             1161..1706
                     /gene="nusG"
                     /locus_tag="ACHOQW_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9572637.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1865..2293
                     /gene="rplK"
                     /locus_tag="ACHOQW_001545"
     CDS             1865..2293
                     /gene="rplK"
                     /locus_tag="ACHOQW_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9572638.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2297..3001
                     /gene="rplA"
                     /locus_tag="ACHOQW_001546"
     CDS             2297..3001
                     /gene="rplA"
                     /locus_tag="ACHOQW_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9572639.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3293..3790
                     /gene="rplJ"
                     /locus_tag="ACHOQW_001547"
     CDS             3293..3790
                     /gene="rplJ"
                     /locus_tag="ACHOQW_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9572640.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3857..4222
                     /gene="rplL"
                     /locus_tag="ACHOQW_001548"
     CDS             3857..4222
                     /gene="rplL"
                     /locus_tag="ACHOQW_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9572641.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4542..8570
                     /gene="rpoB"
                     /locus_tag="ACHOQW_001549"
     CDS             4542..8570
                     /gene="rpoB"
                     /locus_tag="ACHOQW_001549"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9572642.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8647..12870
                     /gene="rpoC"
                     /locus_tag="ACHOQW_001550"
     CDS             8647..12870
                     /gene="rpoC"
                     /locus_tag="ACHOQW_001550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9572643.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13083..13526
                     /locus_tag="ACHOQW_001551"
     CDS             13083..13526
                     /locus_tag="ACHOQW_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFP9572644.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="ACHOQW_001552"
     CDS             complement(13961..15094)
                     /gene="thiH"
                     /locus_tag="ACHOQW_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP9572645.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIAKESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="ACHOQW_001553"
     CDS             complement(15091..15861)
                     /gene="thiG"
                     /locus_tag="ACHOQW_001553"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP9572646.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="ACHOQW_001554"
     CDS             complement(15863..16063)
                     /gene="thiS"
                     /locus_tag="ACHOQW_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP9572647.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="ACHOQW_001555"
     CDS             complement(16047..16802)
                     /gene="thiF"
                     /locus_tag="ACHOQW_001555"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP9572648.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="ACHOQW_001556"
     CDS             complement(16795..17430)
                     /gene="thiE"
                     /locus_tag="ACHOQW_001556"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP9572649.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="ACHOQW_001557"
     CDS             complement(17430..19325)
                     /gene="thiC"
                     /locus_tag="ACHOQW_001557"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP9572650.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19406..19506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="ACHOQW_001558"
     CDS             complement(19558..20034)
                     /gene="rsd"
                     /locus_tag="ACHOQW_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP9572651.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20129..20902
                     /gene="nudC"
                     /locus_tag="ACHOQW_001559"
     CDS             20129..20902
                     /gene="nudC"
                     /locus_tag="ACHOQW_001559"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP9572652.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20942..22006
                     /gene="hemE"
                     /locus_tag="ACHOQW_001560"
     CDS             20942..22006
                     /gene="hemE"
                     /locus_tag="ACHOQW_001560"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP9572653.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22016..22687
                     /gene="nfi"
                     /locus_tag="ACHOQW_001561"
     CDS             22016..22687
                     /gene="nfi"
                     /locus_tag="ACHOQW_001561"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP9572654.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22730..23320
                     /gene="yjaG"
                     /locus_tag="ACHOQW_001562"
     CDS             22730..23320
                     /gene="yjaG"
                     /locus_tag="ACHOQW_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP9572655.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23507..23779
                     /gene="hupA"
                     /locus_tag="ACHOQW_001563"
     CDS             23507..23779
                     /gene="hupA"
                     /locus_tag="ACHOQW_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP9572656.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23792..24487
                     /gene="yjaH"
                     /locus_tag="ACHOQW_001564"
     CDS             23792..24487
                     /gene="yjaH"
                     /locus_tag="ACHOQW_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP9572657.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="ACHOQW_001565"
     CDS             complement(24489..24914)
                     /gene="zraP"
                     /locus_tag="ACHOQW_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP9572658.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25152..26528
                     /gene="zraS"
                     /locus_tag="ACHOQW_001566"
     CDS             25152..26528
                     /gene="zraS"
                     /locus_tag="ACHOQW_001566"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP9572659.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26525..27850
                     /gene="zraR"
                     /locus_tag="ACHOQW_001567"
     CDS             26525..27850
                     /gene="zraR"
                     /locus_tag="ACHOQW_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP9572660.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27847..29136)
                     /gene="purD"
                     /locus_tag="ACHOQW_001568"
     CDS             complement(27847..29136)
                     /gene="purD"
                     /locus_tag="ACHOQW_001568"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9572661.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29148..30737)
                     /gene="purH"
                     /locus_tag="ACHOQW_001569"
     CDS             complement(29148..30737)
                     /gene="purH"
                     /locus_tag="ACHOQW_001569"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9572662.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7310 a         8057 c         8522 g         7238 t
ORIGIN      
        1 ccgatcgaag acgtattctc catctccggt cgtggtaccg ttgttaccgg tcgtgtagaa
       61 cgcggtatca tcaaagttgg tgaagaagtt gaaatcgttg gtatcaaaga gactcagaag
      121 tctacctgta ctggcgttga aatgttccgc aaactgctgg acgaaggccg tgctggtgag
      181 aacgtaggtg ttctgctgcg tggtatcaaa cgtgaagaaa tcgaacgtgg tcaggtactg
      241 gctaagccgg gcaccatcaa gccgcacacc aagttcgaat ctgaagtgta cattctgtcc
      301 aaagatgaag gcggccgtca tactccgttc ttcaaaggct accgtccgca gttctacttc
      361 cgtactactg acgtgactgg taccatcgaa ctgccggaag gcgtagagat ggtaatgccg
      421 ggcgacaaca tcaaaatggt tgttaccctg atccacccga tcgcgatgga cgacggtctg
      481 cgtttcgcaa tccgtgaagg cggccgtacc gttggcgcgg gcgttgtagc aaaagttctg
      541 agctaattgc cgataacatt tgacgcaatg cgcactaaaa gggcatcatt tgatgccctt
      601 tttgcacgct ttcgtaccag aacctggctc atcagtgatt ttctttgtca taatcattgc
      661 tgagacaggc tctgttgagg gcgtataatc cgaaaagcta atacgcgttt cgatttggtt
      721 tgcctcgcga tcgcggggtg aaaatgtttg tagaaaactt ctgacaggtt ggtttatgag
      781 tgcgaatacc gaagctcaag gaagcgggcg cggcctggaa gcgatgaagt gggtcgttgt
      841 ggtggcattg ctcttggtgg cgattgtcgg caactatctt tatcgcgaca ttatgctgcc
      901 gctgcgtgcg ctggccgtag taattctgat tgctgcagcg ggtggtgtcg cgctgttaac
      961 gacaaaaggt aaagctaccg ttgcttttgc ccgtgaagcg cgtaccgaag tccgtaaggt
     1021 catttggccg actcgccagg aaacattgca caccacgctg attgtggctg cggttaccgc
     1081 agtaatgtca ctgatcctgt ggggactgga tggtattctg gttcgcctgg tatcctttat
     1141 cactggcctg aggttctgag atgtctgaag ctcctaaaaa gcgctggtac gtcgttcagg
     1201 cgttttccgg ttttgaaggc cgcgtagcaa cgtcgctgcg tgagcatatc aaattacaca
     1261 acatggaaga tttgtttggt gaagtcatgg taccaaccga agaagtggtt gaaatccgtg
     1321 gcggtcagcg tcgcaaaagc gaacgtaaat tcttccctgg ctacgtcctc gttcagatgg
     1381 tgatgaacga cgcgagctgg cacctggtgc gcagcgtacc gcgtgtgatg ggcttcatcg
     1441 gcggtacttc cgatcgtcct gcgccaatca gcgataaaga agtcgatgcg attatgaacc
     1501 gcctgcagca ggttggtgat aagccgcgtc cgaaaacgct gtttgaaccg ggtgaaatgg
     1561 tccgtgttaa tgatggtccg ttcgctgact tcaacggtgt tgttgaagaa gtggattacg
     1621 agaaatctcg tctgaaagtg tctgtttcta tcttcggtcg tgcgaccccg gtagagctgg
     1681 acttcagcca ggttgaaaaa gcctaaccca gcgatcaaaa aagcggcgat ttaatcgttg
     1741 cacaaggcgc gagattggaa tacaatttcg cgccttttgt ttttatgggt cttgcccgta
     1801 aaacgatttt ttatatcacg gggagcctct cagaggcgtt attacccaac ttgaggaatt
     1861 tataatggct aagaaagtac aagcctatgt caagctgcag gttgcagctg gtatggctaa
     1921 cccgagtccg ccagtaggtc cggctctggg tcagcagggc gtaaacatca tggaattctg
     1981 caaagcgttc aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc cggtagtaat
     2041 caccgtttac gctgaccgtt ctttcacttt cgttaccaag actccgccgg cagcagttct
     2101 gctgaaaaaa gcggctggta tcaagtctgg ttccggtaag ccgaacaaag acaaagtggg
     2161 taaaatttcc cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg acatgactgg
     2221 tgccgacatt gaagcgatga ctcgctccat cgaaggtact gcacgttcca tgggcctggt
     2281 agtggaggac taagaaatgg ctaaactgac caagcgcatg cgtgttatcc gcgagaaagt
     2341 tgatgcaacc aaacagtacg acatcaacga agctatcgct ctgctgaaag agctggcgac
     2401 tgctaaattc gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg ctcgtaaatc
     2461 tgaccagaac gtacgtggtg caactgtact gccgcacggt actggccgtt ccgttcgcgt
     2521 agccgtattt acccagggtg caaacgctga agctgctaaa gctgcaggtg ctgaactggt
     2581 aggtatggaa gatctggctg accagatcaa gaaaggcgaa atgaactttg acgttgttat
     2641 tgcttctccg gatgcaatgc gcgttgttgg ccagctgggc caggttctgg gtccgcgcgg
     2701 cctgatgcca aacccgaaag tgggtactgt aacgccgaac gttgctgaag cggttaaaaa
     2761 cgctaaagct ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc acaccaccat
     2821 cggtaaagtg gactttgacg ctgacaaact gaaagaaaac ctggaagctc tgctggttgc
     2881 gctgaaaaaa gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag ttagcatctc
     2941 caccaccatg ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt ctgtaaacta
     3001 atgcctttac gtgggcggtg attttgtcta caatcttacc cccacgtatg ctaacgaaag
     3061 ttagcagcta aaaagatttg ttcgttggag cctggcctat ccaggcctcc gtcgaagacc
     3121 gcaggagttt cgcaagaaac ttaatcccct gcgtagacgg tgacagaacg ctaagattat
     3181 tcttttatat tctggcttgt ttctgctcac cgtaattaag acgctctctc cgtttggagg
     3241 agtgaagtga gttccagaga ttttctctgg caaacatcca ggagcaaagc taatggcttt
     3301 aaatcttcaa gacaaacaag cgattgttgc tgaagtcagc gaagtagcca aaggcgcgct
     3361 gtctgcagta gttgcggatt cccgtggcgt aactgtagat aaaatgactg aactgcgtaa
     3421 agcaggtcgc gaagctggcg tatacatgcg tgttgttcgt aacaccctgc tgcgccgtgc
     3481 tgttgaaggt actccgttcg agtgcctgaa agacgcgttt gttggtccga ccctgattgc
     3541 atactctatg gaacacccgg gcgctgctgc tcgtctgttc aaagagttcg cgaaagcgaa
     3601 tgcaaaattt gaggtcaaag ccgctgcctt tgaaggtgag ctgatcccgg cgtctcagat
     3661 cgaccgcctg gcaactctgc cgacctacga agaagcaatt gcacgcctga tggcaaccat
     3721 gaaagaagct tcggctggca aactggttcg tactctggct gctgtacgcg atgcgaaaga
     3781 agctgcttaa tcgcagttat ctttttaacg cattcgctta cgtataaact tattctgata
     3841 ttcaggaaca atttaaatgt ctatcactaa agatcaaatc attgaagcag ttgcagctat
     3901 gtctgtaatg gacgttgtag aactgatctc tgcaatggaa gaaaaattcg gtgtttccgc
     3961 tgctgctgct gtagctgtag ctgctggccc ggttgaagct gctgaagaaa aaactgaatt
     4021 cgacgtaatt ctgaaagctg ctggcgctaa caaagttgct gttatcaaag cagtacgtgg
     4081 cgcaactggc ctgggtctga aagaagctaa agacctggta gaatctgcac cggctgctct
     4141 gaaagaaggc gtgagcaaag acgacgcaga agcactgaaa aaagctctgg aagaagctgg
     4201 cgctgaagtt gaagttaaat aagccaaccc ttccggttgc agcctgagaa atcaggctga
     4261 tggctggtga ctttttagtc accagccttt ttgcgctgta aggcaccagt agcgtttcac
     4321 actgtttgac tactgctgtg cctttcaatg cttgtttcta tcgacgactt aatatactgc
     4381 gacaggacgt ccgttctgtg taaatcgcaa tgaaatggtt taagcgtgat agcaacaggc
     4441 attgcggaaa gtgttccatt ttccggtcaa caaaatagtg ttgcacaaac tgtccgctca
     4501 atggacagat gggtcgactt gtcagcgagc tgaggaaccc tatggtttac tcctataccg
     4561 agaaaaaacg tattcgtaag gattttggta aacgtccaca agttctggat gtaccttatc
     4621 tcctttctat ccagcttgac tcgtttcaga aatttatcga gcaagatcct gaagggcagt
     4681 atggtctgga agctgctttc cgttccgtat tcccgattca gagctacagc ggtaattccg
     4741 agctgcaata cgtcagctac cgccttggcg aaccggtgtt tgacgtccag gaatgtcaaa
     4801 tccgtggcgt gacctattcc gcaccgctgc gcgttaaact gcgtctggtg atctatgagc
     4861 gcgaagcgcc ggaaggcacc gtaaaagaca ttaaagaaca agaagtctac atgggcgaaa
     4921 ttccgctcat gacagacaac ggtacctttg ttatcaacgg tactgagcgt gttatcgttt
     4981 cccagctgca ccgtagtccg ggcgtcttct ttgactccga caaaggtaaa acccactctt
     5041 cgggtaaagt gctgtataac gcgcgcatca tcccttaccg tggttcctgg ctggacttcg
     5101 aattcgatcc gaaggacaac ctgttcgtac gtatcgaccg tcgccgtaaa ctgcctgcga
     5161 ccatcattct gcgtgccctg aactacacca cagagcagat cctcgacctg ttctttgaaa
     5221 aagttatctt tgaaatccgt gataacaagc tgcagatgga actggtgccg gaacgcctgc
     5281 gtggtgaaac cgcatccttt gacatcgaag ctaacggtaa agtgtacgta gaaaaaggcc
     5341 gccgtatcac tgcgcgccac attcgccagc tggaaaaaga cgacgtcaaa ctgatcgaag
     5401 tcccggttga gtacatcgca ggtaaagtgg ttgctaaaga ctatattgat gagtctaccg
     5461 gcgagctgat ctgcgcagcg aacatggagc tgagcctgga tctgctggct aagctgagcc
     5521 agtctggtca caagcgtatc gaaacgctgt tcaccaatga tctggatcac ggcccgtata
     5581 tctctgaaac cttacgtgtc gacccaacta acgaccgtct gagcgcactg gtagaaatct
     5641 accgcatgat gcgccctggc gagccgccga ctcgtgaagc agcggaaagc ctgttcgaga
     5701 acctgttctt ctccgaagac cgttatgacc tgtctgcggt tggtcgtatg aagttcaacc
     5761 gttctctgct gcgcgaagaa atcgaaggtt ctggtatcct gagcaaagac gacatcattg
     5821 atgttatgaa aaagctcatc gatatccgta acggtaaagg cgaagtcgat gatatcgacc
     5881 acctcggcaa ccgtcgtatc cgttccgttg gcgaaatggc ggaaaaccag ttccgcgttg
     5941 gcctggtacg tgtagagcgt gcggtgaaag agcgtctgtc tctgggcgat ctggataccc
     6001 tgatgcctca ggatatgatc aacgccaagc cgatttccgc agcagtgaaa gagttcttcg
     6061 gttccagcca gctgtctcag tttatggacc agaacaaccc gctgtctgag attacgcaca
     6121 aacgtcgtat ctccgcactc ggcccaggcg gtctgacccg tgaacgtgca ggcttcgaag
     6181 ttcgagacgt acacccgact cactacggtc gcgtatgtcc aatcgaaacc cctgaaggtc
     6241 cgaacatcgg tctgatcaac tctctgtccg tgtacgcaca gactaacgaa tacggcttcc
     6301 ttgagactcc gtatcgtaaa gtgactgacg gtgttgtaac tgacgaaatt cactacctgt
     6361 ctgctatcga agaaggcaac tacgttatcg cccaggcgaa ctccaacctg gatgaagaag
     6421 gccacttcgt agaagacctg gtaacctgcc gtagcaaagg cgaatccagc ttgttcagcc
     6481 gtgaccaggt tgactacatg gacgtatcca cccagcaggt ggtatccgtc ggtgcgtccc
     6541 tgatcccgtt cctggaacac gatgacgcca accgtgcatt gatgggtgcg aacatgcaac
     6601 gtcaggccgt tccgactctg cgtgctgata agccgctggt tggtactggt atggaacgtg
     6661 ctgttgccgt tgactccggt gtaactgcgg ttgctaaacg tggtggtgtc gttcagtacg
     6721 tggatgcttc ccgtatcgtt atcaaagtta acgaagacga gatgtatccg ggtgaagcag
     6781 gtatcgacat ctacaacctg accaaataca cccgttctaa ccagaacacc tgtattaacc
     6841 agatgccgtg tgtgtctctg ggtgaaccgg ttgaacgtgg cgacgtgctg gcagacggtc
     6901 cgtccaccga cctcggtgaa ctggcgcttg gtcagaacat gcgcgtagcg ttcatgccgt
     6961 ggaatggtta caacttcgaa gactccatcc tcgtatccga gcgtgttgtt caggaagacc
     7021 gtttcaccac catccacatt caggaactgg cgtgtgtgtc ccgtgacacc aagctggggc
     7081 cagaagagat caccgctgac atcccgaacg tgggtgaagc tgcgctctcc aaactggatg
     7141 aatccggtat cgtttatatt ggtgcggaag tgaccggtgg cgacattctg gttggtaagg
     7201 ttacgccgaa aggtgaaact cagctgaccc cagaagaaaa actgctgcgt gcgatcttcg
     7261 gtgagaaagc gtctgacgtt aaagactctt ctctgcgcgt accaaacggt gtatccggta
     7321 cggttatcga cgttcaggtc tttactcgcg atggcgtaga aaaagacaaa cgtgcgctgg
     7381 aaatcgaaga aatgcagctc aaacaggcga agaaagacct gtctgaagaa ctgcagatcc
     7441 tcgaagcggg tctgttcagc cgtatccgtg ctgtgctggt agccggtggc gttgaagctg
     7501 agaagctcga caaattgccg cgcgatcgct ggctggagct gggcctgacc gacgaagaga
     7561 aacaaaatca gctggaacag ctggctgagc agtatgacga actgaaacac gagttcgaga
     7621 agaaactcga agcgaaacgc cgcaaaatca cccagggcga cgatctggca ccgggcgtgc
     7681 tgaagattgt taaggtatat ctggcggtta aacgccgtat ccagcctggt gacaagatgg
     7741 caggtcgtca cggtaacaag ggtgtaattt ctaagatcaa cccgatcgaa gatatgcctt
     7801 acgatgaaaa cggtacgccg gtagacatcg tactgaaccc gctgggcgta ccgtctcgta
     7861 tgaacatcgg tcagatcctc gaaacccact tgggtatggc tgcgaaaggt atcggcgaca
     7921 agatcaacgc catgctgaaa cagcagcagg aagtcgcgaa actgcgtgaa ttcatccagc
     7981 gtgcgtacga tctgggcgct gacgttcgtc agaaagttga cctgagtacc ttcagcgatg
     8041 aagaagttat gcgtctggct gaaaacctgc gcaaaggtat gccaatcgca acgccggtgt
     8101 tcgacggtgc gaaagaagca gaaattaaag agctgctgaa acttggcgac ctgccgactt
     8161 ctggtcagat ccgcctgtac gacggccgca ctggtgaaca gttcgaacgt ccggtaaccg
     8221 ttggttacat gtacatgctg aaactgaacc acctggtcga cgacaagatg cacgcgcgtt
     8281 ccaccggttc ttacagcctg gttactcagc agccgctggg tggtaaggca cagttcggtg
     8341 gtcagcgttt cggggagatg gaagtgtggg cgctggaagc atacggcgca gcatacaccc
     8401 tgcaggaaat gctcaccgtt aagtctgatg acgtgaacgg tcgtactaag atgtataaaa
     8461 acatcgtgga cggcaaccat cagatggagc cgggcatgcc agaatccttc aacgtattgt
     8521 tgaaagagat tcgttcgctg ggtatcaaca tcgaactgga agacgagtaa ttctcgctca
     8581 aacaggtcac tgctgtcggg gtaaaacccg gcagcggatt gtgctaactc cgacgggagc
     8641 aaatccgtga aagatttatt aaagtttctg aaagcgcaga ctaaaaccga agagtttgat
     8701 gcgatcaaaa ttgctctggc ttcgccagac atgatccgtt catggtcttt cggtgaagtt
     8761 aaaaagccgg aaaccatcaa ctaccgtacg ttcaaaccag aacgtgacgg ccttttctgc
     8821 gcccgtatct ttgggccggt aaaagattac gagtgcctgt gcggtaagta caagcgcctg
     8881 aaacaccgtg gcgtcatctg tgagaagtgt ggcgttgaag tgacccagac taaagtacgc
     8941 cgtgagcgta tgggccacat cgaactggct tccccgactg cgcacatctg gttcctgaaa
     9001 tcgctgccgt cccgtatcgg cctgctgctc gatatgccgc tgcgcgatat cgaacgcgta
     9061 ctgtactttg aatcctatgt ggttatcgaa ggcggtatga ccaacctgga gcgtcagcag
     9121 atcctgactg aagagcagta tctggacgcg ctggaagagt tcggtgacga attcgacgcg
     9181 aaaatgggtg cggaagcaat ccaggccctg ctgaagagca tggatctgga gcaagagtgc
     9241 gaacagctgc gtgaagagct gaacgaaacc aactccgaaa ccaagcgtaa aaagctgacc
     9301 aagcgtatca aactgctgga agcgttcgtt cagtctggta acaaaccaga gtggatgatc
     9361 ctgaccgttc tgccggtact gccgccagat ctgcgtccgc tggttccgct ggatggtggt
     9421 cgtttcgcga cttctgacct gaacgatctg tatcgtcgcg tcattaaccg taacaaccgt
     9481 ctgaaacgtc tgctggatct ggctgcgccg gacatcatcg tacgtaacga aaaacgtatg
     9541 ctgcaggaag cggtagacgc cctgctggat aacggtcgtc gcggtcgtgc gatcaccggt
     9601 tctaacaagc gtcctctgaa atctttggcc gacatgatca aaggtaaaca gggtcgtttc
     9661 cgtcagaacc tgctcggtaa gcgtgttgac tactccggtc gttctgtaat caccgtgggt
     9721 ccatacctgc gtctgcatca gtgcggtctg ccgaagaaaa tggcgctgga gctgttcaaa
     9781 ccgttcatct acggcaagct ggaactgcgt ggtcttgcta ccaccattaa agctgcgaag
     9841 aaaatggttg agcgcgaaga agctgtcgtt tgggatatcc tggacgaagt tatccgcgaa
     9901 cacccggtac tgctgaaccg tgcaccgact ctgcaccgtc tgggtatcca ggcatttgaa
     9961 ccggtactga tcgaaggtaa agctatccag ctgcacccgc tggtttgtgc ggcatataac
    10021 gccgacttcg atggtgacca gatggctgtt cacgtaccgc tgacgctgga agcccagctg
    10081 gaagcgcgtg cgctgatgat gtctaccaac aacatcctgt ccccggcgaa cggcgaacca
    10141 atcatcgttc cgtctcagga cgttgtactg ggtctgtact acatgacccg tgactgtgtt
    10201 aacgccaaag gcgaaggcat ggtgctgact ggcccgaaag aagcagaacg tctgtatcgc
    10261 tctggtctgg cttctctgca tgcgcgcgtt aaagtgcgta tcaccgagta tgaaaaagat
    10321 gctaacggtg aattagtagc gaaaaccagc ctgaaagaca cgactgttgg ccgtgccatt
    10381 ctgtggatga ttgtaccgaa aggtctgcct tactccatcg tcaaccaggc gctgggtaaa
    10441 aaagcaatct ccaaaatgct gaacacctgc taccgcattc tcggtctgaa accgaccgtt
    10501 atttttgcgg accagatcat gtacaccggc tttgcatatg cagcgcgttc tggtgcatct
    10561 gttggtatcg atgacatggt catcccggag aagaaacacg aaatcatctc cgaggcagaa
    10621 gcagaagttg ctgaaattca ggagcagttc cagtctggtc tggtaactgc gggcgaacgc
    10681 tacaacaaag ttatcgatat ctgggctgcg gcgaacgatc gtgtatccaa agcgatgatg
    10741 gataacctgc aaactgaaac cgttattaac cgtgacggtc aggaagagaa gcaggtttcc
    10801 ttcaacagca tctacatgat ggccgactcc ggtgcgcgtg gttctgcggc acagattcgt
    10861 cagcttgctg gtatgcgtgg tctgatggcg aagccggatg gctccatcat cgaaacgcca
    10921 atcaccgcga acttccgtga aggtctgaac gtactccagt acttcatctc cacccacggt
    10981 gctcgtaaag gtctggcgga taccgcactg aaaactgcga actccggtta cctgactcgt
    11041 cgtctggttg acgtggcgca ggacctggtg gttaccgaag acgattgtgg tacccatgaa
    11101 ggtatcatga tgactccggt tatcgagggt ggtgacgtta aagagccgct gcgcgatcgc
    11161 gtattgggtc gtgtaactgc tgaagacgtt ctgaagccgg gtactgctga tatcctcgtt
    11221 ccgcgcaaca cgctgctgca cgaacagtgg tgtgacctgc tggaagagaa ctctgtcgac
    11281 gcggttaaag tacgttctgt tgtatcttgt gacaccgact ttggtgtatg tgcgcactgc
    11341 tacggtcgtg acctggcgcg tggccacatc atcaacaagg gtgaagcaat cggtgttatc
    11401 gcggcacagt ccatcggtga accgggtaca cagctgacca tgcgtacgtt ccacatcggt
    11461 ggtgcggcat ctcgtgcggc tgctgaatcc agcatccagg tgaaaaacaa aggtagcatc
    11521 aagctcagca acgtgaagtc ggttgtgaac tccagcggta aactggttat cacttcccgt
    11581 aacaccgaac tgaaactgat cgacgaattc ggtcgtacca aagaaagcta caaagtacct
    11641 tacggtgcgg tactggcaaa aggcgatggc gaacaggttg ctggcggcga aaccgttgca
    11701 aactgggacc cgcacaccat gccggttatc accgaagtaa gcggttttgt acgctttact
    11761 gacatgatcg acggccagac cattactcgt cagaccgacg aactgaccgg tctgtcttcg
    11821 ctggtggttc tggattccgc agaacgtacc gcaggtggta aagatctgcg tccggcactg
    11881 aaaatcgttg atgctcaggg taacgacgtt ctgatcccag gtaccgatat gcctgcgcag
    11941 tacttcctgc cgggtaaagc gattgttcag ctggaagatg gcgtacagat cagctctggt
    12001 gacaccctgg cgcgtattcc gcaggaatcc ggcggtacca aggacatcac cggtggtctg
    12061 ccacgcgttg cggacctgtt cgaagcacgt cgtccgaaag agccggcaat cctggctgaa
    12121 atcagcggta tcgtttcctt cggtaaagaa accaaaggta aacgtcgtct ggttatcacc
    12181 ccggtagacg gtagcgatcc gtacgaagag atgattccga aatggcgtca gctcaacgtg
    12241 ttcgaaggtg aacgtgtaga acgtggtgac gtaatttccg acggtccgga agcgccgcac
    12301 gacattctgc gtctgcgtgg tgttcatgct gtgactcgtt acatcgttaa cgaagtacag
    12361 gacgtatacc gtctgcaggg cgttaagatt aacgataaac acatcgaagt tatcgttcgt
    12421 cagatgctgc gtaaagctac catcgttaac gcgggcagct ccgacttcct ggaaggcgaa
    12481 caggttgaat actctcgcgt caagatcgca aaccgcgaac tggaagcgaa cggcaaagtg
    12541 ggtgcaactt actcccgcga tctgctgggt atcaccaaag cgtctctggc aaccgagtcc
    12601 ttcatctccg cggcatcgtt ccaggagacc actcgcgtgc tgaccgaagc agccgttgcg
    12661 ggcaaacgcg acgaactgcg cggcctgaaa gagaacgtta tcgtgggtcg tctgatcccg
    12721 gcaggtaccg gttacgcgta ccaccaggat cgtatgcgtc gccgtgctgc gggtgaagct
    12781 ccggctgcac cgcaggtgac tgcagaagac gcatctgcca gcctggcaga actgctgaac
    12841 gcaggtctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg
    12901 cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag
    12961 gaatttctga atactcataa tcaatgtaga gatgactaat atcctgaaac tgactgaact
    13021 aattgagtca aactcggcaa ggattcgata ctattcctgt gtagctttct taaggaacga
    13081 gaatgaaaca ggaagtggag aagtggcgac cttttaaaca tccggatggc gacgttcgtg
    13141 atttatcgtt tcttgatgct catcaggtta tctacgttca gcatcatgag gacaaggagc
    13201 ctttagagta tcgcttttgg gttacctact ctcttcactg cttcacaaaa gattatgaac
    13261 atcagacgaa cgaagaaaaa ctatcgttaa tgtaccacgc gcctaaagaa tctcgtccct
    13321 tctgccagca ccgttataac ttagcgcgca cacacttaaa aagaactatt ttggcgctgc
    13381 cagaaagcaa cgttattcat gccgggtatg gtagctatgc cgtgattgag gtggacttag
    13441 acggaggaga taaggcattt tactttgttg tgttcagggc tttcagtgaa atttttcacc
    13501 attgcctaaa acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca
    13561 ccttaaggtg ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt
    13621 tgccttgacc ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca
    13681 tcctggaaga tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc
    13741 tcttacaatc agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg
    13801 aatttttaag aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct
    13861 gctgacggca ctaccataaa aaaacacaac gctttcaaca accttgcgtt tttcatcttc
    13921 cgtagcggct cggaataaat tttcgttctt acattccgtc tcatagtctt tgcgaggcgc
    13981 gtcccagata gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg
    14041 cagcaaccgc ttccggtctg cgctcgtcgt gcggtgagaa ctgttccagc tcggggtgat
    14101 tgtcggcata gccacctggc tgcgttttcg aaaaagcgct gacgttatta attgccagcg
    14161 gaataacgcg atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg
    14221 gtgcaagcag ccggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg
    14281 caggctcaat gccgccagta catggacgca ggcgtgggaa ggagacagag tagcggcttt
    14341 gccagtaatg ctgttgcagc catagcaaat gttctgcaac catatagcag tcaacgcgcc
    14401 agctgtcgga aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacggccca
    14461 gccgatccgg cgtttccagc cgccagaaga agtcctgttt tttgcctttc agatgatggc
    14521 gggcataagt cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagtt
    14581 gctttaactc ggcgtattcc gtctccgcca gcggctgtac ttccatctgt agtgaagaga
    14641 actgttcacg cagggcaggg agatgacgac gaaagtaatc catccccact ttcgcctgat
    14701 gttcaccggt gactaaaagc agatgttcaa agcccatctc ccgtatagcg gcgctttcct
    14761 tggcaatatc cgcttcatcc agcgttttgc gcttgatgcg gttgctcata gagaatccgc
    14821 agtacgtgca gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg
    14881 tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc
    14941 cactggcggc tggcgataac agcgccatca tatcgtcgcg ggtgagttgc gaggcattta
    15001 gcgcccgctc tacgtcagca gccgttttgc cgttgatacg caggcggatg tcgtcccagt
    15061 ccagttgtcg ccagcgatcg ctgaaggttt tcataccgat gcctccagaa atccggtcag
    15121 cgggctggtg gcatgagcaa aataactgcg gctgcccggt ccggactgag gtgccagcag
    15181 gcctgcttct actgccagac gaaatgcctt cgccatgttg acgggatcgt ccgcgacggc
    15241 aatcgccgta ttcactaaca ccgcgtcggc ccccatttcc agcgcctgcg cggcatggct
    15301 gggaacgccg atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc
    15361 cagcatggcg cgggtttcca gcccctgatt cgagccaatc ggcgcgccga gcggcatcac
    15421 cgctgcacag ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg
    15481 cagcacgaca aatccctgtt gtaccagcgt ttcggcggct ttcagggttt cgatgggatc
    15541 gggcaacagc cagcgggcgt cagggtgaat ctccaatttt aaccagtttg tgcctaacgc
    15601 ttcacgagcc agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggatgtatt
    15661 tggcagcagg gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg
    15721 caagtcgaca cgtttcatcg ccagcgtcac cagctggctg ccggaagcgc ggattgcctc
    15781 taccatcagt tgtgaagagg cgaatttccc ggtgccggta aacagatgtg aatcaaacgt
    15841 tttgtccgca atacgtaaca tctcaacccc ctgcaataac ctgaaaaagt aggatctggt
    15901 cgccatcctg cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg
    15961 ccagagccgc gcccgcttgt cgttgaacga gttgctccag tagttcgtga acagtttgcc
    16021 cggcggcgca ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc
    16081 gggcaaccac tggcgcggcg caacgccagg ctgcgccact ggctcgactt accgtcgaac
    16141 agtcggagtt ctcccgcagg cgtctctata ccgcttaata acttaatggc ttccagtgcc
    16201 tgcaaagtgc ccataacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt
    16261 tctggctcct ggttttctgg ccacaggcag cggtaacaac cctgccccca gggcggcgtc
    16321 agtaccatca actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt
    16381 gccacgcagg cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc
    16441 acatcggctt gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct
    16501 gtcagttgaa tatcgggatt caactgtgtc agtcgctgat ggctgacctg cgatttcggg
    16561 cgatcgatat cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg
    16621 tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg ccaggtatag cgcagcaggt
    16681 gtacccagcc cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg
    16741 tcaagagcga tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc
    16801 atcgccaact cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg
    16861 agtaatggcg ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg
    16921 tgccagactg ataccgccaa tcgccacggt gggataatcc gacagtcgct caacatgccg
    16981 tgccagctgt tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac
    17041 atgtcccagc gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc
    17101 atgggtcgaa acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg
    17161 caaatcttcc tgccccaaat ggacgccata cgcctgatgc ttgatcgcga gccgccagta
    17221 atcgttgata aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc
    17281 ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc
    17341 atccaacaga cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa
    17401 aggtacagga ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct
    17461 ccgcctctgg cacggaagtt ctccgacata tccgctattc ccatttcaat agtttgcgtg
    17521 gcggcgtaat cacgcacttc ctggctgatt ttcatcgagc agaatttcgg cccacacatg
    17581 gagcaaaaat gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg
    17641 gcggtaaacg ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct
    17701 ttcgacatgg cgttatcgcg aatttgtgcg cccggatgcc ctttcgccag gtcagcggcg
    17761 tgggcggcaa tcttataggt gataagcccc tgcttaacat cttctttatt aggcagaccc
    17821 agatgctctt ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc
    17881 gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc
    17941 agagtgtaaa acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc
    18001 atctgcatcg gcacgtggcc tgggccttca atcatcacct gcacatcata ttcccaggcg
    18061 atttttgtca gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc
    18121 tgaatagaac cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag
    18181 atttcacaaa tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac
    18241 catttcgcca taatcgaacc gccgcgcgag acgataccgg tcaggcgttt cgcggtcatc
    18301 ggcacatagc gcagcagcac ccccgcatgg atagtgaagt aatccacacc ttgctcggcc
    18361 tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccattaacc
    18421 ttctccagcg cctggtagat cggcactgta ccaatcggca ccgggctgtt acgcaaaatc
    18481 cactcgcggg tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc
    18541 cagcgcgtgg accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag
    18601 ttgccgatat tggcgttaac ttttaccagg aaattgcgac caataatcat cggctccgat
    18661 tccggatgat taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca
    18721 aattccgcag tgatattttc cggcagacgt gcgccaaagc tcattcccgg atgctggtgg
    18781 cgtaaaactt cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc
    18841 atttccggtg tgatgatgcc ctggcgggcg tagtgcagtt gtgtgacgcg gcgtcctgct
    18901 ttggcgcgtt ttggcgttaa tacgccgcta aaacgcagct cgtcgaggcc atcatctgcc
    18961 agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca
    19021 tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc gatctgcgga
    19081 tcgccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc
    19141 tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc
    19201 acgccgggtt gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc
    19261 agggtgtcga taaaatgttg ggcctgggcg cgttgttcgc gacgggtcag ttttgttgca
    19321 gacatagctc attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag
    19381 acgatcgccg caaaggcgat gcgatagcag attaactctt gttcccttcg caggtattag
    19441 cctgatcagg ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa
    19501 gaaattagcc ccacaaacgg ggcgttgaat gtaaattacg cgttaacagc gcagaactca
    19561 agcaggatgt ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac
    19621 aaagcgcgct tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt
    19681 atcatgatcg atagcggttt ccaggctgga atcgtagtaa tccataatct gttgggtatt
    19741 ggcttcgagt tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt
    19801 atgaagaatg cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg
    19861 acaaaaatca tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat
    19921 gccaaccaga ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac
    19981 cagtttgtta cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga
    20041 ctccgcaagt ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg
    20101 ccagtgatgc aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg
    20161 ctggtgggtc gtcagccatg aacaaaaatt atggttgccg aagggagaat tgccatatgg
    20221 cgaagcggca aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg
    20281 ggaacctgtt tggttaatac aacagcagcg gcgttacgat atggggtcgg tacgtcaggt
    20341 cattgatctc gatgttgggc tgtttcaact ggccggacgt ggcgtacaac tggcggagtt
    20401 ttaccgatcg cataaatact gtggttactg cgggcatgaa atgtatccaa gcaaaaccga
    20461 atgggcgatg ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat
    20521 tattgttgcc atccgtcgcg atgattcgat cctcctcgcc cagcataccc gccatcgtaa
    20581 cggtgtccat acggtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcggt
    20641 cgcgcgggaa gtgatggaag agagcggaat taaagttaaa cacttgcgtt atgtgacttc
    20701 ccagccgtgg ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg
    20761 cgacatcgtg atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt
    20821 gccgttactc ccgccgcccg gcaccgtagc gcgccgtctg atagaagata cggtggcgat
    20881 gtgtcgggca gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa
    20941 aatgaccgaa cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt
    21001 cactcctgta tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg
    21061 cgcccaggcg ggcgatttta tgtcgctgtg caaaaacgcc gagctggcgt gtgaagtgac
    21121 cttgcaaccg ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac
    21181 cgtcccggac gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac
    21241 ctcgccagtc acctgcaaag ctgacgtcga taaactgcca attccggacc cggaagatga
    21301 actggggtac gtgatgaacg cggtgcgtac cattcgtcgc gaactgaaag gcgaagtgcc
    21361 gctgattggt ttttccggca gcccgtggac gctggcgacc tacatggtgg aaggcggcag
    21421 cagcaaagcc ttcaccgtga tcaaaaaaat gatgtatgcc gatccgcagg cgctgcacgc
    21481 tctactcgat aaactggcga aaagcgtcgc tttgtatctg aatgcgcaga ttaaagccgg
    21541 tgctcaggct gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca
    21601 acagttctcg ctttattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg
    21661 tcgccgcgta ccggtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc
    21721 agaaaccggt tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg
    21781 ccgtgtgggc aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc
    21841 gcctgcccgc attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg
    21901 tcatgtcttt aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt
    21961 gttcgtggag gcagtgcatc gactgtctga acagtatcac cgctaaggag cgattatgga
    22021 tctcgcgtca ttacgcgctc aacaaatcga actggcttct tctgtgatcc gcgaggatcg
    22081 actcgataag gatccgccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg
    22141 agaagtgacg cgagcggcga tggtgctgct gaaatacccc tcgcttgagc tggtggagta
    22201 taaagtggcc cgcatcgcta ccaccatgcc ttacattcca ggttttcttt ccttccgcga
    22261 atatcctgcg ctgctggcag cgtgggagat gctgtcacaa aagccggatt tagtgtttgt
    22321 cgatggtcat gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggtttgct
    22381 ggtggatgtg ccgaccattg gcgtagcgaa aaaacgacta tgcggtaaat tcgaaccgct
    22441 ctccagcgaa ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt
    22501 ctggcgcagc aaagcgcgct gtaacccgct atttatcgct actggtcatc gggtcagcgt
    22561 ggacagcgcg ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac
    22621 gcgctgggcg gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca
    22681 gccctaattc aggtaaactg cggataattt ccgtatttga gaactcaaca tgttacaaaa
    22741 cccaattcat ctgcgtctgg aacgcctgga aagctggcag catgtcactt tcatggcttg
    22801 cttatgcgaa cgcatgtacc ccaattacgc catgttctgc cagcaaaccg gttttggtga
    22861 tgggcaaatt taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagatgc
    22921 aaaagtaaat ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga
    22981 tttcgatctg tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt
    23041 ccattcgcgt ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat
    23101 cacgaccgtt gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg atgaagagct
    23161 caaagaaaac ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt
    23221 agctgagtgc gaagaacgcg atatcgagct gataaaaggc cttagggcag acctgcgtga
    23281 ggcggatgag agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa
    23341 cgcctgattt gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg
    23401 ggcgaaaaaa agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt
    23461 cgcactcgat gcttagcaag cgataaacac attgtaagga taacttatga acaagactca
    23521 actgattgat gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct
    23581 ggagtccact ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt
    23641 tggtttcggt accttcaaag tgaaccaccg cgctgagcgt actggccgca acccgcagac
    23701 cggtaaagaa atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact
    23761 gaaagacgca gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc
    23821 accccttttg tctttctggc gtcgatcatt gatgctggct ggcgctctgt ttctcactgc
    23881 ttgtagtcac aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg
    23941 cgcggtacgc atctggcgaa aagacagcgg cgataatgtg catcttcttg ccgtgtttag
    24001 cccgtggcgc agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac
    24061 gctcatcaat atcaatgttt acagcaaacc cccggtgaat attcgcgcgc gttttgacga
    24121 tcgcggtgat ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa
    24181 cgaccaaatc gatttatacc gttatcgggc tgatcagatc cgccagatta gcgatgcctt
    24241 acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac
    24301 cacctgcgaa gggcaaacca ttaaacctga tttagattcg caggcaatag cgcatatcga
    24361 gcgccgccag agccgctctt ctgttgatgt cagcgtggca tggctggaag cgcccgaagg
    24421 ttcgcaatta ctgttagtgg caaactctga tttctgtcgc tggcaaccca acgagaaaac
    24481 gttctgattt accagtggcc catacccata tgaccgccac caccgcagcc gccgtagccc
    24541 atgcccattc cggctccgcg cggaataccc gcttcagcca tcgcaatatc ccgtttcacc
    24601 cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg
    24661 ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt
    24721 tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc
    24781 tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca
    24841 aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc
    24901 gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg
    24961 tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac
    25021 tcgcctgctg cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag
    25081 cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg
    25141 caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg
    25201 cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg
    25261 attatggccg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta
    25321 ttcgtgctct cgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc
    25381 agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg
    25441 atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt
    25501 ccccgcagga aatgcagcag ttacatccgg gagatgaaga agtgtggcgg cggatcgata
    25561 gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg
    25621 gaatgcaccg gatgcgccat atgcagcagt atgccgcgac accacaagca attttcatcg
    25681 ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta
    25741 tcctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc
    25801 gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg
    25861 cgctggggca tcttgcggca ggcgttgccc acgaaattcg taacccgctt tcctcgatta
    25921 aagggctggc aaaatacttt gccgagcgcg cgccagcagg gggagaagcg catcaactgg
    25981 cgcaggtgat ggcgaaagaa gctgaccgtt taaaccgcgt ggtaagcgag ctgctggaac
    26041 tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact
    26101 cattacagct ggtaagccag gatgcaaact gccgggagat ccagttacgc tttaccgcca
    26161 acgacacgtt gccggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc
    26221 tttatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg acggccagcg
    26281 aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc
    26341 agcttgaagc catcttcacc ccgtacttca ccaccaaagc cgaaggcacc ggactggggc
    26401 tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagcctgg
    26461 agggaaaagg cgcaaggttc accctctggc ttccggtcaa tattacgcgt aaggacccac
    26521 aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac
    26581 tattttgcag gctttgctgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg
    26641 acaggcgctg gagcaggtgc gggagcgggt ttttgatctt gtgctttgcg atgtgcgaat
    26701 ggcggagatg gacggcattg ccacgctaaa agagatcaaa gcgttaaatc cggcgattcc
    26761 ggtactgatt atgaccgcat actctagcgt cgagacggcg gtagaggcgt tgaaaaccgg
    26821 ggcgctggat tatctcatca agccgctgga tttcgataac ctgcaggcga cgctggaaaa
    26881 ggcgctcgcg catacgcaca ttattgatgc tgaaacgcct gcggtgactg ccagccagtt
    26941 cggtatggtc ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc
    27001 gccatcggaa gccacggtgc tgatccacgg cgattccggc accggtaaag agctggtcgc
    27061 cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc
    27121 ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac
    27181 tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgttgtttct
    27241 cgatgaaatt ggcgatatct cgccgatgat gctggtgcgt ctgctgcgtg cgattcagga
    27301 gcgcgaagtt cagcgtgtcg gtagcaacca gactatttcg gttgatgtcc ggctgattgc
    27361 cgcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta
    27421 ctatcgcctg aacgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat
    27481 tcctctgctg gctggccatt ttctgcagcg ctttgctgaa cgtaatcgca aggcggtaaa
    27541 aggctttacg ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg
    27601 tgaactggaa aatgcggtgg aacgggcggt ggtgctgctg actggggaat atatttccga
    27661 acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctggcacaaa gcctggatat
    27721 tcagccgctg gtggaagtcg aaaaagaagt gattctggcg gcactggaga aaacgggcgg
    27781 caacaaaacc gaagccgccc gtcagttagg gatcacgcgc aaaacgctgt tggcaaaatt
    27841 gtcgcgttag ttctgctcgc gttcgatagc gcgccagccg atatctttcc ggcagaagca
    27901 gtcgtcccag tggatatcgg tcattaaggc ataggcgcgt ttctgtgctt ctgctacggt
    27961 atgacccagc gcggtgacgc acagtacgcg cccgccgctg gttaccacct gctcgtcatc
    28021 cgccagtttt gtgcccgcgt ggaacacttt gccgtcttcc acttcttcca gcggcaggcc
    28081 gtggatcacg tcaccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc
    28141 gagagaagca cgttcatccc actctgacgt tttctcgtct agtttgcctt cacaggccgc
    28201 caggcagagt tcaacaagat cggacttcat gcgcagcata atcggctggg tttctggatc
    28261 gccaaagcgg cagttaaatt cgataacctt cggattgccc tgtttgtcga tcatcaggcc
    28321 cgcgtagaga aaaccggtgt aggtgttgcc ttccgacgcc atgcctttca cggttggcca
    28381 gatgatacgt tccatcgtgc gctgatgaac gtcatctgtc actaccggcg caggagagta
    28441 agcgcccatt ccgcccgtgt tcggcccggt atctttatcg cctacgcgtt tgtgatcctg
    28501 gctggtggcc atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc
    28561 gccgtcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaacg cgttgcccgc
    28621 cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt
    28681 cccggcagcc agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc
    28741 cagcgcaggt tctacctcgg tgaagttctg atattccgcc gtaggaatgt tatggcgggc
    28801 caggaaatct ttagtaaacg ctttcgagcc ttccagctgt gcggcgcctg cggttgggcc
    28861 gaagattttc atcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc
    28921 cgggccgacg atggttagat ctaccttttc gttttgcgcg aaatccagca gcgccgggat
    28981 atcggtcacg ccgatagcga cgttttgcag cgtgggttcc agcgctgtgc ctgcgttacc
    29041 cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg
    29101 ctcgcgcccg ccgttaccaa tcactaatac tttcatctgt cgctccatta atggcggaag
    29161 tggcgcatat cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg
    29221 tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca
    29281 tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc
    29341 aggccttcat cggcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg
    29401 cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg
    29461 aacttcgcca ctttccagca gaacagcgca tcacgcagtt cctgttcggt cggctgacgt
    29521 ttggtgacga cgcgcagttc ttccgcaccg accatgccca gatcgcgatc ctgaaccagc
    29581 agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag
    29641 gtcagaacgc gtacattctg tttggcggcg gtgattttca gggcttcttc gctggcggac
    29701 ggcgcaataa tcacttcaac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc
    29761 agctcgcggt taaaggcaat gatgccgccg aatgcggagg tcgggtcggt tttgtacgcg
    29821 cgatcgtaag catcaagaat tgaattgccg atagccacgc cgcaagggtt ggcgtgcttc
    29881 acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg
    29941 gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct
    30001 tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc
    30061 atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct
    30121 ttgctttcac cgtgataagc cggaaccatg ctgccgaagt agttggcaat catgctgtcg
    30181 taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc
    30241 gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg
    30301 attgcgacat ctttatggtt cttggcggcg gagcgcacca tcgttgggcc gccgatatcg
    30361 atgttctcaa ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga
    30421 tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg
    30481 tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt
    30541 ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt
    30601 tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt
    30661 gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact
    30721 ggacgacgtt gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga
    30781 atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat
    30841 tgtaacgaaa acgtttgcgc aacgctcgcg aattttttta attcgatggc gatcacgatt
    30901 ttgcccccga ttagcgtggg agaaatagag aaataacgct gatgattgtg gataactctg
    30961 tgcgtaaaaa ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg
    31021 caatttttct attgcggcct gcggagaact ccctataatg cgcctccatc gacacggcgg
    31081 atgtgaatca cttcacacaa acagccggtt cggttgaaga gaaaaat
//
LOCUS       DAZKFZ010000038        31022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000038 DAZKFZ010000000
VERSION     DAZKFZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(37..3111)
                     /gene="lacZ"
                     /locus_tag="ACHOQW_001570"
     CDS             complement(37..3111)
                     /gene="lacZ"
                     /locus_tag="ACHOQW_001570"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP9572663.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(3234..4316)
                     /gene="lacI"
                     /locus_tag="ACHOQW_001571"
     CDS             complement(3234..4316)
                     /gene="lacI"
                     /locus_tag="ACHOQW_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP9572664.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(4353..5306)
                     /locus_tag="ACHOQW_001572"
     CDS             complement(4353..5306)
                     /locus_tag="ACHOQW_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFP9572665.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(5326..6102)
                     /locus_tag="ACHOQW_001573"
     CDS             complement(5326..6102)
                     /locus_tag="ACHOQW_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9572666.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(6217..7050)
                     /gene="mhpR"
                     /locus_tag="ACHOQW_001574"
     CDS             complement(6217..7050)
                     /gene="mhpR"
                     /locus_tag="ACHOQW_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP9572667.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            7241..8905
                     /gene="mhpA"
                     /locus_tag="ACHOQW_001575"
     CDS             7241..8905
                     /gene="mhpA"
                     /locus_tag="ACHOQW_001575"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP9572668.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            8907..9851
                     /gene="mhpB"
                     /locus_tag="ACHOQW_001576"
     CDS             8907..9851
                     /gene="mhpB"
                     /locus_tag="ACHOQW_001576"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP9572669.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            9869..10735
                     /gene="mhpC"
                     /locus_tag="ACHOQW_001577"
     CDS             9869..10735
                     /gene="mhpC"
                     /locus_tag="ACHOQW_001577"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP9572670.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            10745..11554
                     /gene="mhpD"
                     /locus_tag="ACHOQW_001578"
     CDS             10745..11554
                     /gene="mhpD"
                     /locus_tag="ACHOQW_001578"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP9572671.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            11551..12501
                     /gene="mhpF"
                     /locus_tag="ACHOQW_001579"
     CDS             11551..12501
                     /gene="mhpF"
                     /locus_tag="ACHOQW_001579"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP9572672.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            12498..13511
                     /gene="mhpE"
                     /locus_tag="ACHOQW_001580"
     CDS             12498..13511
                     /gene="mhpE"
                     /locus_tag="ACHOQW_001580"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP9572673.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            13687..14898
                     /gene="mhpT"
                     /locus_tag="ACHOQW_001581"
     CDS             13687..14898
                     /gene="mhpT"
                     /locus_tag="ACHOQW_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP9572674.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            15000..15539
                     /gene="yaiL"
                     /locus_tag="ACHOQW_001582"
     CDS             15000..15539
                     /gene="yaiL"
                     /locus_tag="ACHOQW_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP9572675.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(15664..16497)
                     /gene="fghA"
                     /locus_tag="ACHOQW_001583"
     CDS             complement(15664..16497)
                     /gene="fghA"
                     /locus_tag="ACHOQW_001583"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9572676.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(16590..17699)
                     /gene="frmA"
                     /locus_tag="ACHOQW_001584"
     CDS             complement(16590..17699)
                     /gene="frmA"
                     /locus_tag="ACHOQW_001584"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP9572677.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(17734..18009)
                     /gene="frmR"
                     /locus_tag="ACHOQW_001585"
     CDS             complement(17734..18009)
                     /gene="frmR"
                     /locus_tag="ACHOQW_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP9572678.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(18169..19215)
                     /gene="fbpC"
                     /locus_tag="ACHOQW_001586"
     CDS             complement(18169..19215)
                     /gene="fbpC"
                     /locus_tag="ACHOQW_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFP9572679.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(19227..21305)
                     /locus_tag="ACHOQW_001587"
     CDS             complement(19227..21305)
                     /locus_tag="ACHOQW_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9572680.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(21374..22405)
                     /locus_tag="ACHOQW_001588"
     CDS             complement(21374..22405)
                     /locus_tag="ACHOQW_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9572681.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(22402..23718)
                     /gene="uhpC"
                     /locus_tag="ACHOQW_001589"
     CDS             complement(22402..23718)
                     /gene="uhpC"
                     /locus_tag="ACHOQW_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9572682.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(23791..25332)
                     /locus_tag="ACHOQW_001590"
     CDS             complement(23791..25332)
                     /locus_tag="ACHOQW_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9572683.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(25332..25961)
                     /locus_tag="ACHOQW_001591"
     CDS             complement(25332..25961)
                     /locus_tag="ACHOQW_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9572684.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            26264..27226
                     /gene="tauA"
                     /locus_tag="ACHOQW_001592"
     CDS             26264..27226
                     /gene="tauA"
                     /locus_tag="ACHOQW_001592"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9572685.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIATPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            27239..28006
                     /gene="tauB"
                     /locus_tag="ACHOQW_001593"
     CDS             27239..28006
                     /gene="tauB"
                     /locus_tag="ACHOQW_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP9572686.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            28003..28830
                     /gene="tauC"
                     /locus_tag="ACHOQW_001594"
     CDS             28003..28830
                     /gene="tauC"
                     /locus_tag="ACHOQW_001594"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP9572687.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            28827..29678
                     /gene="tauD"
                     /locus_tag="ACHOQW_001595"
     CDS             28827..29678
                     /gene="tauD"
                     /locus_tag="ACHOQW_001595"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP9572688.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(29785..30759)
                     /gene="hemB"
                     /locus_tag="ACHOQW_001596"
     CDS             complement(29785..30759)
                     /gene="hemB"
                     /locus_tag="ACHOQW_001596"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP9572689.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         7136 a         8224 c         8578 g         7084 t
ORIGIN      
        1 gcgaaatacg ggcagacatg gcctgcccgg ttattattat ttttgacacc agaccaactg
       61 gtaatggtag cgaccggcgc tcagctggaa ttccgccgat actgacgggc tccaggagtc
      121 gtcgccacca atccccatat ggaaaccgtc gatattcagc catgttcctt cttccgcgtg
      181 cagcagatgg cgatggctgg tttccatcag ttgttgttgg ctgtagcggc tgatgttgaa
      241 ctggaagtcg ccgcgccact ggtgtgggcc ataattcaat tcgcgcgtcc cgcagcgcag
      301 accgttttcg ctcgggaaga cgtacggggt atacatgtct gacaatggca gatcccagcg
      361 gtcaaaacag gcggcagtaa ggcggtcggg atagttttct tgcggcccta atccgagcca
      421 gtttacccgc tctgctacct gcgccagctg gcaggtcagg ccaatccgtg ccggatgcgg
      481 tgtatcgctc gctacttcaa catcaacggt aatcgccatt tgaccactac catcaatccg
      541 gtaggttttc cggctaataa ataaggtttt tccctgatgc tgccatgcgt ggacagtggt
      601 aatcagcacc gcgtcggcaa gtgtatctgc cgtgcactgc aacaacgccg cttctgcctg
      661 gtaatggccc gcagccttcc agcgttcgac ccaggcgtta gggtcaatgc gggtcgcttc
      721 acttacgcca atgtcgttat ccagcggtgc gcgggtgaac tgatcgcgca gcggcgtcag
      781 cagttgtttt ttatcgccaa tccacatctg tgaaagaaag cctgactggc ggttaaattg
      841 ccaacgctta ttatccagct cgatgcaaaa atccgtttcg ctggtggtca gttgcgggat
      901 ggcgtggggc gcggagggga gtgtcacgct gaggttttcc gccagacgcc actgctgcca
      961 ggcgctgatg tgtccggctt ctgaccatgc ggtcgcgttc ggttgcacta cgtgtaccgt
     1021 tagccagagt tgcccggtgc tctccagtcg cggtagttca ggcaattcaa ttacctgttt
     1081 accttgcgga gcgacatcca gaggcacttc accgcttgcc agcggcttac catccagcgc
     1141 caccgtccag tgcaggagct cgttatcgct atgacggaac aggtattcgc tggtcacttc
     1201 gatggttcgc ccggataaac ggaactggaa aaactgctgc tggtgttttg cttccgtcag
     1261 cgccggatgc ggcgtgcggt cggcaaagac cagaccgttc atgcagaact ggcgatcatt
     1321 cggcgtatcg ccaaaatcac cgccgtaagc cgaccacgga ttgccatttt catcatattt
     1381 aatcagcgac tgatccacca agtcccagac gaagccgccc tgtaaacggg ggtactgacg
     1441 aaacgcctgc cagtatttag cgaagccgcc aagactgtta cccatcgcgt gggcgtattc
     1501 gcaaaggatc agtgggcgca tttctccagg cagcgaaagc cattttttga tggaccattt
     1561 cggcacagcc gggaagggct ggtcttcatc cacgcgcgcg tacatcgggc aaataatatc
     1621 ggttgcggag gtgtcggctc cgccgccttc atactgcacc gggcgggatg gatcgacaga
     1681 tttaatccag cgatagagtg cgtcgtgatt agcgccgtgg cctgactcat tccccagcga
     1741 ccagatgatc acactcgggt gattacgatc gcgctgcacc attcgtgtta ctcgttcgct
     1801 catggccggt aaccagcgcg gatcatcggt cagacgattc attggcacca tgccgtgggt
     1861 ttcaatattg gcttcatcca ccacatacag gccgtagcgg tcgcacaggg tgtaccacag
     1921 cggatggttc ggataatgcg aacagcgcac ggcgttaaag ttgttctgct tcattagcag
     1981 gatatcctgc accatcgtct gctcatccat gacctgacca tgcagaggat gatgctcgtg
     2041 acggttaacg ccgcgaatca gcaacggctt gccgttcagc agcagcagac cattttcaat
     2101 ccgcacctcg cggaaaccga catcgcaggc ttctgcttca atcagcgtgc cgtcggcggt
     2161 gtgcagttca accaccgcac gatagatatt cgggatttcg gcgctccaca gtttcgggtt
     2221 ttcgacgttc agacctagtg tgacgcgatc ggcataaccg ccacgctcat cgataatttc
     2281 accgccgaaa ggcgcggtgc cgctggcgac ctgcgtttca ccctgccaca aagaaaccgt
     2341 cacccgcagc tcatcgcgca gctcgccgta catctgaact tctgcctcca gtaccgcgcg
     2401 gctaaaatca tcattaaaga gagtggcaac atggaaatcg ctgatttgtg tggtcggttt
     2461 gtgcagcaac gagacgtcac ggaaaatgcc gctcatccgc cacatatcct gatcttccag
     2521 ataactgccg tcactccagc gcagcaccat caccgcgagg cggttttctc cggcgcgtaa
     2581 aaatgcgctc aggtcaaatt cagacagcaa acgactgtcc tggccgtaac cgacccagcg
     2641 cccgttgcac cacagatgaa acgccgagtt aacgccatca aaaataattc gcgtctggcc
     2701 ttcctgtagc cagctttcat caacattaaa tgtgagcgag taacaacccg tcggattctc
     2761 cgtgggaaca aatggcggat tgaccgtaat gggatatgtc acgttggtgt agatgggcgc
     2821 gtcgtaaccg tgcatctgcc agtttgaggg gacgacgaca gtatcggcgt caggaagatc
     2881 gcactccagc cagctttccg gaaccgcttc tggtgccgga aaccagacaa attgccactc
     2941 accattcagg ctgcgcaact gctgggaagg gcgattggtg cgggcctctt cgctattacg
     3001 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     3061 ccagtcacga cgttgtaata cgacggccag tgaatctgta atcatagtca tagctgtatc
     3121 ctgtgtgaaa ttgttatccg ctcacaattt cacacaacat acgagccgga cgcatagagt
     3181 gtaaagcgtg gggtgcctaa tgagtgagct gactcacatt aattgcgttg cgctcactgc
     3241 ccgctttcca gtcgggaaac ttgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3301 ggagaagcgg tttgcgtctt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     3361 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     3421 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacacgag
     3481 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg caacccggac
     3541 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     3601 ggaacgatgc cctcatttag catttgcatg gtttgttgaa aaccggacat ggcactccag
     3661 tcgccctccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagccc
     3721 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     3781 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     3841 atactgttga tgggagtctg gtcagagaca tcaagaaata acgccggaac attagcgcag
     3901 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     3961 acgcgttgcg cgagaagatt gtgcactgcc gctttacagg cttcgacgcc gcttcgttct
     4021 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     4081 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     4141 ttccccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     4201 tccacttttt cccgcgtttt cgcagaaacg tggctggcct gattcaccac gcgggaaacg
     4261 gtctgataag agacaccggc atactctgcg acatcgtata gcgttactgg tttcacattc
     4321 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
     4381 cattcggcgg ggctgacgcc gaagtgagat ttaaaatgct gacgccaggt cacaggtgag
     4441 agaaaaccca cctcagccgc tatccgctca acgggcaact gactggatcc caacagttcc
     4501 tggctacggc gtaagcgttc agtaatgagc cattcggcga tactcgaacc ggtcgctttc
     4561 ataaaatggc gagttaatgt gcggcgactc atcattactc gctgcgccag cgagtcgaga
     4621 tcatgctgtt gcgcaatgtt ttgccgcagg taatccagca ggaggtttat gcgttcatca
     4681 tgggtatttt tcggcaccgg ttgctcaata aactgcgcct gaccgccggt gcgatatggc
     4741 ggtacgacca tccgtcgggc aatatggtta gcatagtcgc tgccaaaatg ttgccgaaca
     4801 atatacaaac agcaatccag cgccgcggca gtaccggcag aggtaataat gccgtcatcg
     4861 tctacgtaca acgcgttaat atccagatgt gtttgtggaa aacgtgcctg aaattcacgc
     4921 tcgaactccc agtgcgtggc ggcacgctta tttttcagta aacctgcata gccgagcaca
     4981 aacgtgccca ggcaaagccc gacaatctgt gcgccgttat cccgtgcggt cgttaaggct
     5041 tccagcagtt tttgtggcgg tttttgtgta atggttcccc agtaaggaat tatcacaata
     5101 tctgcctgga tgactgcggt atagtcatgg gtggcggtta cggaaaaccc atcggcagag
     5161 tccacgtttc ccggcttttc agcgcaaata atcacgtgaa agcgtttttt ctccgccatc
     5221 ttttcactaa acacgataaa cggtacggaa aggtgaaaag gactaaaacc ggcaacggcg
     5281 ataatggcaa cagttagcgg gcgcataaca ctctttatct ccgaattaga atgtcaacgt
     5341 ttcaccatct tctggtacgt tcacaaattc gataatttga ttatctctgg cgtattcctt
     5401 gagcgcatca cgcgtcagca aacagtggtt tacggcctcc atatgtgtcg cgataatttg
     5461 cgcttctggg agggtaaaat gtgttttgag aatatcttct gctcccataa ttatcgcgcc
     5521 atgccccaga acatgcgcaa atccggcatt caacacaacc acttctggtt ggaaagttag
     5581 cattgtagcc tcgacgtcgt cacgccagag ggtatcgcca gccagataga gcgttttctc
     5641 tgtcgggtgc tggaaaacaa cgccgcaagc tgcgcctaaa cgcttcgcca tcgccgggtc
     5701 ggcataggtg cgatcggagc cgtgctgccc tgtcgtttta tgcagagtaa tatcgccaaa
     5761 tttagtctcg tcggtaagta cggtaagatg cgtaaatcct tgtttattca gtgattcagc
     5821 atcttgctca ttttgcacat aaatcagttt atctttaggg acgacgcggg aggctgtttc
     5881 gtcccagtga tcttcatgca aatgggtcac gattagggcg tcaacgttga gtaacgttgt
     5941 taaatcgcac ggtaattcta ccgttggatt gcggatttct gcgcgtgccg ttccggcaaa
     6001 acccggataa cttcctttag gcgcgagtaa cgggtcgata agaaatgttt tgccagcgta
     6061 ttggataatt tgggtggcat tacgaatttg cgtaattttc atgatttgct ccacatcaca
     6121 gtattgatgt cattagcgta gcgtgcagcc cgtactgtgc atgtgggcaa atgggcgaat
     6181 aacgatgaga atgggccaaa ttacacattg gcgtaactag cgtaaatgca cgccgcttcg
     6241 ccgtccggcc accagaatag cctgcgattc aaccccttct tcgatctgtt ttgctacccg
     6301 ttgtagtgcc ggaagatgct tttccgctgc ctgttcgatg gtcattgcgc tcgccatata
     6361 caccagattc agacagccaa tcacccgttg ttcactgcgc agcggtacgg cgatagaggc
     6421 gatcttctcc tcctgatccc agccgcggta gttctgtccg taaccctctt tgcgcgcgcg
     6481 cgccagaatg gcttccagct ttaacggttc tcgtgccagt tgatagtcat caccggggcg
     6541 ggaggctaac atctcgatta attccttgcg ttcttgttcc gggcaaaagg ccagccaggt
     6601 caggcccgag gcggttttca gaagcggtaa acgccgcccg accattgccc ggtgaaagga
     6661 taagcggctg aaacggtgag tggtttcgcg taccaccatt gcatcaacat ccagcgtgga
     6721 cacatctgtc ggccatacca cttcgcgcaa cagatcgccc agcagtgggg ccgccagtgc
     6781 agaaatccac tgttcgtcac gaaatccttc gcttaattgc cgcactttga tggtcagacg
     6841 aaaactatca tcggagggac tacgccggac atatccctct tcctgcagcg tctccagcag
     6901 tcgccgcaca gtggtgcgat gcaggccgct gagttccgcc agcagcccga cgctggcacc
     6961 gccgtcaagt ttatttaaca tatttaataa cattagaccg cgggttaagc cgcgcacggt
     7021 tttgtattcc gtctgctcat tgttctgcat attaattgac atttctatag ttaaaacaac
     7081 gttgtgcacc tggtgcacat tcgggcatgt tttgattgta gccgaaaaca ccctttctat
     7141 actgagcgca caataaaaaa tcatttacat atttttaaca aaataagttg cgctgtactg
     7201 tgcgcgcaac ggcattttgt ccgagtcgtg aggtactgaa atggcaatac aacaccctga
     7261 catccagcct gctgttaacc atagcgttca ggtggcgatc gctggtgccg gtccggtcgg
     7321 gctgatgatg gcgaactatc tcggccagat gggcattgac gtgctggtgg tggagaaact
     7381 cgataagttg atcgactacc cgcgtgcgat tggtattgat gacgaggcgc tgcgcaccat
     7441 gcagtcggtc ggcctggtcg ataatgttct gccgcatact acgccgtggc acgcgatgcg
     7501 ttttctcacc ccgaagggcc gctgttttgc tgatattcag ccaatgaccg atgaatttgg
     7561 ctggccgcgc cgtaacgcct ttattcagcc gcaggtcgat gcggtgatgc tggaaggggt
     7621 gtcgcgtttt ccgaatgtgc gctgcttgtt ttcccgcgag ctggaggcct tcagtcagca
     7681 agatgacgaa gtgaccttgc acctgaaaac ggcagaaggg ctgcgggaaa tagtcaaagc
     7741 ccagtggctg gtggcctgtg atggtggggc aagttttgtc cgtcgcaccc tgaatgtgcc
     7801 gtttgaaggt aaaactgcgc caaatcagtg gattgtggta gatatcgcca acgatccgtt
     7861 aagtacgccg catatctatt tgtgttgcga tccggtgcgc ccgtatgttt ctgccgcgct
     7921 acctcatgcg gtacgtcgct ttgaatttat ggtgatgccg ggagaaaccg aagaacagct
     7981 gcgtgagccg caaaatatgc gcaagctgtt aagcaaagtg ctgcctaatc cggacaatgt
     8041 tgaattgatt cgccagcgtg tctacaccca caacgcgcga ctggcgcaac gtttccgtat
     8101 tgatcgcgta ctgctggcgg gcgatgccgc gcacatcatg ccggtatggc aggggcaggg
     8161 ctataacagt ggtatgcgcg acgcctttaa cctcgcatgg aaactggcgt tggttatcca
     8221 ggggaaagcc cgcgatgcgc tgctcgatac ctatcaacaa gaacgtcgcg atcacgccaa
     8281 agcgatgatt gacctgtccg tgacggcggg caacgtgctg gctccgccga aacgctggca
     8341 gggtacgtta cgtgacggcg tttcctggct gctgaattat ctgccgccag taaaacgcta
     8401 cttcctcgaa atgcgcttca agccgatgcc gcaatattac ggcggtgcgc tggtgcgtga
     8461 gggcgaagcg aagcactctc cggtcggcaa gatgtttatt cagccgaaag tcacgctgga
     8521 aaacggcgac gtgacgctgc tcgataacgc gatcggcgcg aacttcgcgg taattggctg
     8581 gggatgcaat ccactgtggg ggatgagcga cgagcaaatc cagcagtggc gcgcgttgag
     8641 cacacgcttc attcaggtgg tgccggaagt gcaaattcat accgcacagg ataaccacga
     8701 cggcgtacta cgcgtggggg atacgcaagg tcgcctgcgt agctggttcg cgcaacacaa
     8761 tgcttcgctg gtggtgatgc gcccggatcg ctttgttgcc gccaccgcca ttccgcaaac
     8821 actgggcaag accctgaata aactggcgtc ggtgatgacg ctgacccgcc ctgatgccga
     8881 cgtttctgtc gaaaaggtag cctgatatgc acgcttatct tcattgtctt tcccactcgc
     8941 cgctggtggg gtatgtcgac ccggcacaag aggtactcga tgaggtcaat ggcgtgattg
     9001 ccagcgcccg cgagcgtatt gcggctttct cccctgaact ggtggtgctg tttgcgccag
     9061 atcactacaa cggctttttc tatgacgtga tgccaccgtt ctgtttaggc gttggagcga
     9121 cggcaattgg tgatttcggc agtgcggcag gagagctgcc cgtgcctgtg gagctggcgg
     9181 aggcctgtgc gcatgccgtc atgaagagcg ggatcgatct tgccgtttct tactgtatgc
     9241 aggtggacca cgggttcgcc cagccgctgg agttcctgct cggtgggctg gataaggtgc
     9301 cagttctgcc tgtgttcatc aacggtgtcg ccacgccgct gcccggtttc cagcgtaccc
     9361 gcatgttggg tgaagccatt ggacgtttca ccagcactct caataaacgc gtgctgttcc
     9421 taggttccgg tgggctttcc catcagccgc cggtgcccga actggcgaaa gccgatgccc
     9481 atatgcgcga ccgcctgttg gggagcggga aagatttacc cgccagtgag cgcgaattgc
     9541 gtcagcaacg ggtgattagc gccgctgaga agtttgttga ggatcagaga actctgcatc
     9601 cgctcaaccc gatttgggat aaccagttca tgactttgct ggagcaggga cgcatacagg
     9661 aactggatgc cgtcagtaac gaagagcttt ccgccattgc cggaaagtcg acacatgaaa
     9721 tcaaaacctg ggtcgccgct tttgccgcta tttctgcatt tggcaactgg cgtagcgaag
     9781 ggcgttatta ccgcccaatc ccggagtgga ttgccggatt tggctcgtta agcgccagaa
     9841 cagagaactg aatatgcagg agaagatgat gagttatcag ccacaaaccg aagccgccac
     9901 cagccgtttt ctgaatgtag aagaagcggg taaaacgctg cgcatccatt ttaatgactg
     9961 cggacaaggc gacgaaaccg ttgtcctgct gcatggttcc ggcccgggtg ctactggctg
    10021 ggcgaacttc agccgcaata tcgatccgct ggtagaggcg ggctatcggg tgatcctgct
    10081 ggattgtccg ggttggggca agagcgattc gatcgttaat agtggttcgc gatcggatct
    10141 taatgcacga atcctgaaaa gcgtggtgga tcaactggat atcgccaaaa tccacctgct
    10201 gggcaactcg atgggtggcc atagttctgt ggcgttcacc cttaactggc cggagcgcgt
    10261 cggcaaactg gtgctgatgg gcggcggtac gggcggcatg agtttgttta cgccgatgcc
    10321 aaccgaaggt attaagcgac tgaatcagct ttatcgtcag ccgactatcg aaaacctgaa
    10381 gctgatgatg gatatcttcg tttttgatac cagcgatttg accgacgccc tgtttgaagc
    10441 gcgcctgaat aatatgctgt cgcgccgcga tcacctggaa aacttcgtta agagcctgga
    10501 agctaatccg aaacagttcc cggattttgg cccacgtctg gcggaaatca aagcgcaaac
    10561 cctgattgtc tgggggcgca acgaccgctt tgtgccgatg gatgcgggtc tgcgtctgct
    10621 gtccggcatt gccggttctg aactgcatat cttccgcgac tgtgggcatt gggcgcagtg
    10681 ggaacatgcc gacgctttca atcaactggt gctgaatttc ctcgcacgtg cttaaggaac
    10741 ggtcatgacg aagcatactc ttgagcaact ggcggcggat ttacgccgcg ccgcagagca
    10801 gggcgaagcg attgcgccgc tgcgcgatct gattggtatc gataacgctg aagcggccta
    10861 cgccattcag cacataaatg tgcaatatga cgttgcgcag gggcgtcgcg tggtggggcg
    10921 taaagtgggc ctgacacatc cgaaagtgca gcaacaactg ggcgttgatc aaccggattt
    10981 cgggacatta tttgccgaca tgtgttatgg cgataacgaa atcattcctt tttcccgtgt
    11041 tctgcaaccc cgcattgaag cggagatcgc actggtgttg aaccgcgatt tacccgcaac
    11101 tgatatcacc ttcgacgaat tgtataacgc cattgaatgg gtacttccgg cgctggaggt
    11161 ggtgggtagc cgcattcgcg actggtcgat tcagtttgtc gataccgtgg cagataacgc
    11221 ttcctgcggg gtgtatgtca tcggcggtcc ggcgcaacgt ccggcggggt tagacctgaa
    11281 aaactgcgcc atgaagatga cgcgtaataa cgaagaggtt tccagcgggc gcggcagcga
    11341 atgcctggga catccgctta atgccgccgt ctggctggca cgcaaaatgg ccagtctggg
    11401 tgaaccgctg cgcgccggag atatcattct taccggggca ttaggtccga tggtggcagt
    11461 gaatgcgggc gatcgttttg aagcccatat tgaaggcata ggttcagttg ctgcggcatt
    11521 ttcaagcgca gccccaaaag gaagtctgtc atgagtaagc gtaaagtcgc cattatcggt
    11581 tctggcaaca ttggcaccga tctgatgatt aaaattttgc gtcacgatca gcatctggag
    11641 atggcggtga tggtcggcat tgatcctcag tccgacggtc tggcgcgtgc cagacgtatg
    11701 ggcgtcgcca ccacccatga aggggtgatc gggctgatga acatgcctga atttgctgat
    11761 atcgacattg tatttgatgc gaccagcgcc ggtgctcatg tgaaaaacga tgcggctttg
    11821 cgcgaagcga aaccggatat tcgcttaatt gacctgacgc ctgctgccat cggcccttac
    11881 tgcgtgccgg tggttaacct cgaggagaac gtcgatcaac tgaacgtcaa catggtcacc
    11941 tgcggcggcc aggccaccat tccaatggtg gcggcagttt cacgcgtggt gcgtgttcat
    12001 tacgccgaaa ttatcgcttc tatcgccagt aaatctgccg gacctggcac gcgtgccaat
    12061 atcgatgaat ttacggaaac cacttcgcga gccattgaag tggtgggcgg cgcggcaaaa
    12121 gggaaggcga ttatcgtgct taacccagca gagccaccgt tgatgatgcg tgacacggtg
    12181 tatgtattga gcgacgaagc ttcacaagat gatatcgaag cctcaatcaa tgaaatggct
    12241 gaggcggtgc aggcttacgt accgggatat cgcctgaaac agcgcgtgca gtttgaagtt
    12301 atcccgcagg ataaaccggt taatttgccg ggcgtagggc agttctccgg gctgaaaacg
    12361 gcggtctggc tggaagtcga aggcgcagcg cattatctgc ctgcctatgc gggcaacctc
    12421 gacattatga cttccagtgc gctggcgaca gcagaaaaaa tggcccagtc actggcgcgc
    12481 aaggcaggag aagcggcatg aacggtaaaa aactttatat ctcggacgtc acgttgcgtg
    12541 acggtatgca cgccattcgt catcagtatt cgctggaaaa cgttcgccag attgccaaag
    12601 cactggacga tgcccgcgtg gattcgattg aagtggccca cggcgacggt ttgcaggggt
    12661 ccagctttaa ctatggtttc ggcgcacata gcgaccttga atggattgaa gcagcggcgg
    12721 atgtggtgaa gcacgccaaa atcgcgacgt tgttgctgcc aggaatcggc actattcacg
    12781 atctgaaaaa tgcctggcag gctggcgcgc gggtggttcg tgtggctacg cactgtaccg
    12841 aagctgatgt ttccgcccag catattcagt atgcccgcga gctcggaatg gacaccgttg
    12901 gttttctgat gatgagccat atgaccacgc cggagaatct cgcaaagcag gcaaagctga
    12961 tggaaggcta cggtgcgacc tgtatttatg tggtggattc tggcggtgcg atgaacatga
    13021 gcgatatccg tgaccgtttc cgcgccctga aagcagtgct gaaaccagaa acgcaaaccg
    13081 gcatgcacgc tcaccataac ctgagtcttg gcgtggcgaa ctctatcgcg gcggtggaag
    13141 agggctgcga ccgaatcgac gccagcctcg cgggaatggg agcgggcgca ggtaacgcgc
    13201 cgctggaagt atttattgcc gccgtggata aactgggttg gcagcatggg gccgatctct
    13261 atgcgttaat ggatgctgct gacgacctgg tgcgtccgtt gcaggatcga ccggtacgag
    13321 tcgatcgcga aacgctggcg ctgggatacg ctggtgttta ctccagcttc ctgcgtcact
    13381 gtgaaacggc ggcggcgcgt tatggcttaa gcgcggtgga tattctcgtt gagctgggca
    13441 aacgccggat ggttggcggc caggaggata tgatcgtcga cgtggcgctg gatctgcgca
    13501 acaacaaata atattgactg ccgagaacgt gcattttgta tgcccatccc agcacgactg
    13561 ccggatgcgg cgtgaacgcc ttatccggtc tacatttcgc ccgtaggcag tcattaaata
    13621 gttctgatta cgcggctggc aatgtatcag tcgcgattca cctgcacacg caatgaggtt
    13681 catcttatgt cgactcgtac cccttcatca tcttcatccc gcctgatgct gaccatcggg
    13741 ctttgttttt tggtcgctct gatggaaggg ctggatcttc aggcggctgg cattgcggcg
    13801 ggtggcatcg cccaggcttt cgcactcgat aaaatgcaaa tgggctggat attcagcgcc
    13861 ggaatactcg gtttactacc cggcgcgttg gttggcggaa tgctggcgga ccgttatggt
    13921 cgcaagcgca ttttgattgg ctcagttgcg ctgtttggtt tgttctcgct ggcaacggcg
    13981 attgcctggg atttcccctc actggtcttt gcgcggctga tgaccggtgt cgggctgggg
    14041 gcggcgttgc cgaatcttat cgccctgacg tctgaagccg cgggtccacg ttttcgtggg
    14101 acggcagtga gcctgatgta ttgcggtgtt cccattggcg cggcgctggc ggcgacactg
    14161 ggtttcgcgg gggcaaactt agcatggcaa acggtgtttt gggtaggtgg tgtggtgccg
    14221 ttgattctgg tgccgctatt aatgcgctgg ctgccggagt cggcggtttt cgctggcgaa
    14281 aaacaggctg cgccaccact gcgtgcgtta tttgcgccag aaacggcaac cgcgacgctg
    14341 ctgctgtggc tgtgttattt cttcactctg ctggtggttt acatgttgat caactggcta
    14401 ccgctgcttt tggtggagca aggattccag ccatcgcagg cggcaggggt gatgtttgca
    14461 ctgcaaatgg gtgcggcgag cgggacgtta atgttgggcg cattgatgga taagctgcgt
    14521 ccagtaacca tgtcgctact gatttatagc ggcatgttag cttcgctgct ggcgcttgga
    14581 acggtgtcgt catttaacgg tatgttgctg gcgggatttg tcgcggggtt gtttgcgaca
    14641 ggtgggcaaa gcgttttgta tgccctggca ccgttgtttt acagttcgca gatccgcgca
    14701 acaggtgtgg gaacagccgt ggcggtaggg cgtctggggg ctatgagcgg tccgttactg
    14761 gccgggaaaa tgctggcatt aggcactggc acggtcggcg taatggccgc ttctgcaccg
    14821 ggtattcttg ttgctgggtt ggcggtgttt attttgatga gtcggagatt aagaatgcag
    14881 ccgtgtgccg atgcctgacg cgccttatta agcaggggcg aaagggactt gcccccaata
    14941 cacccacggc gtttacaggt atactcgcta aaaattattc agcgggtttg gaaacaaaga
    15001 tggcaaaact taccttacaa gagcagttgc tcaaagcagg attagtcacc agcaaaaaag
    15061 cggcgaaggt cgagagaacg gcgaaaaaat cgcgcgttca ggcgcgtgaa gctcgggcgg
    15121 cggtagaaga aaataaaaag gcacagcttg agcgtgataa acagcttagc gaacagcaaa
    15181 aacaagcggc gttggcgaaa gaatataaag ctcaggtgaa gcagcttatt gaaatgaacc
    15241 ggatcaccat tgccaatggc gatattggtt ttaacttcac tgatggcaat ctgattaaga
    15301 agatttttgt cgataagctc acacaggcgc agttgattaa tggtcgtctg gcgattgccc
    15361 gcttgttggt cgataacaat agcgaaggtg aatacgccat tattcccgcc agcgttgccg
    15421 ataaaattgc tcagcgtgat gccagcagta ttgtgttgca cagcgcgctc agtgccgaag
    15481 agcaggatga agacgacccg tatgccgact tcaaagtgcc tgatgatttg atgtggtaaa
    15541 catagataag tcgtgcgcag atgcctgatg cgacgctatg cgcgtcttat catgcctacg
    15601 gcctgcgggc gaaatgtagg cggataaggc gttcacgccg catccggcag tcgtgcacta
    15661 ttatcaacgc atattcagtt tattggcgtg gtaggcaata tgctcgccaa taaaactgga
    15721 gacaaaataa tagctgtggt cgtaaccctc gtgataacgg attaacgttt tgatattcat
    15781 ctcctggcag atcttttcaa gattttgagt ccgcagctgt tccgcgtaaa aatcatcact
    15841 caacccctga tcaaccatga tttccgcaac gcgttgacct tgtgaaataa gactcaccgg
    15901 atcgtaatcc aaccaggcct ctttattttc accaagatat gcggcaaagg cttgctgtcc
    15961 ccacggtact tgcgatgggg agacaatggg cgaaaacgcc gagacgctga catattcatc
    16021 tgggttacgt aacgccagca ccagcgcgcc cagtccgccc atagaatgac cagagataga
    16081 ctttttggcc gttgccggaa aatgattcat cactaaatcc ggcagctcgt tgcggatata
    16141 gtcatacatt ttgtaatgtt cattccacgg cgcttgcgtc gcgttcaggt aaaacccggc
    16201 accttgcccg agatcgtaac ggtcggcatc tgcgacatga ctgcctcgcg gactggtgtc
    16261 cggcgcaaca acaataatgt tgtgctcagc cgcgtaacgc tgcacccccg atttagtaat
    16321 gaaattctgc tcattgcagg tcaggcccga aagccagtac agcaccggta atttttcacc
    16381 tgcagctttt ggtgggagat agacgccgac attcatttca catttcagtg atgcggaata
    16441 atgccgatac acattttgcc agccgccaaa gctggcatgt ttttcaatga gttccatcag
    16501 tcattatctc acggttaatc ccccggacgg ctgcccggag agagtattaa gaaccccgtc
    16561 tgaagtggac ggggtaaacc agcgggaaat cagtaacgaa ttacggttcg aatggatttg
    16621 ccttcatgca tcaggtcgaa ggcgtcatta atttcatcca ggctcatggt atgcgtgaca
    16681 aacggttcca gatcgatatc acctttcatc gcatcttcaa ccatacccgg taactgggaa
    16741 cgacctttca cgccgccaaa cgcggaacct ttccatacgc gaccagtgac caactggaat
    16801 ggacgggtgg agatttcctg accggaaccc gcgaccccga tgatcaccga ctgaccccag
    16861 ccgcggtgcg cactttccag cgccgcacgc atcacgttga cattaccgat gcattcaaag
    16921 gtatggtcga taccccattt gttgatatcc agcaggacat ctttaatcgg tttgtcgtag
    16981 tcattcgggt taatgcagtc ggtggcaccg aagcgacgcg ccagatcgaa tttcttcggg
    17041 ttggtatcga tagcgataat ccgtcccgct ttcgcctgac gcgcgccctg aaccactgcc
    17101 agaccaatcg cgccaagacc aaacacagca acagaatccc ctggctggac tttagctgtg
    17161 ttgtgtaccg cgccaatacc ggtggtcacg ccacagccca gcaggcagac gtgttcatgg
    17221 tttgcttctg gattaatttt tgccagagac acttccgcga ctacggtgta ttcactgaat
    17281 gtagaacacc ccatgtagtg ataaagcggc tgcccattgt aagaaaaacg ggtggtgccg
    17341 tctggcatca ggcctttacc ctgggtttcg cgaaccgcaa cacagaggtt agttttgcca
    17401 gaacgacaga actcacactc gccgcactct gcggtgtaaa gcgggatcac atggtcgcct
    17461 ggtttgacgc tggttacgcc ttcaccgact tcaaccacaa cgccagcccc ttcgtgaccg
    17521 agaaccaccg ggaatacacc ttccgggtca tcgccggaga gggtaaatgc gtcggtatgg
    17581 caaacgccgg tatgggtgac tttaatcagc acttcacctt ttttcggtgg tgcaacgtca
    17641 atttcaacga tttccagcgg tttaccggga gcaaatgcaa cggcagcacg tgatttcatc
    17701 tctcgctctt cctcaatatg gtaatagatt cagctattta agataggcac gaaccagttc
    17761 aatagtgtcg tcaacggatt ggctgacttc gcggctgtag cagtcatttc ggtcaaacgt
    17821 ttcccggata tggctttcaa gcacttctgc catcagccca ttagccgcgc cccgaacggc
    17881 agcgatctgt tggagtatgg cacggcattc ggcatcaccc tccagcgacc gttccagagc
    17941 atcaatctgc cctcgaatac gacgaactcg agtaaggacc tttttcttct cttccggagt
    18001 actgggcatt tcgcacctca tcatctgcac gcaatatact ataggggggt atgctatatg
    18061 tcaatgcata cccccctata gtatataaca atggagaata attgttgtcc gaagcctaac
    18121 caggtgaacg gtccctcaag acagatgggg accgtttatg tgctattttt atgccgcatc
    18181 cgctaaaaca aacatcccgt aaggatggat ttcgaggtaa tagtgctcac caatatcggg
    18241 ctgtaaacgg gtggcgttta cttgtaataa aatctcctgt ccatgccact ctacggtcac
    18301 ttcgtactgc ggccccatgt aggcgacatg gcgaataaca caacgctgac tctcttcgcc
    18361 gtgattgctt aaggtgatgg cttccgggcg aacgccgacg gtgccgctac cttgcaccgg
    18421 gaaatgcgcc gcgcgcggca ggcgataacc gtagatatcg acatactctt cgctaaagtt
    18481 cgccgggaac aggttggcgt cgcccataaa acttgccata aagcgggagg ctggctgacg
    18541 atagagatcc tgcggtgagc caatctgcat gatgtgacct ttattcatca ccagcacagt
    18601 gtcggaaacc gcgaaggcct cactttgatc gtgagtcacg tagagcgacg tgatattaaa
    18661 ctgcttttgc agctcgcgga tcttatcgcg catgctgcgg cgcaggttgg catcgaggtt
    18721 acttaacggc tcatcaaaca gcagcacctt tggtttgagg atcaacgcgc gggccagcgc
    18781 cacgcgctgt tgctgaccac cggaaatctg gtcgacatag cggtcctcga acccttccag
    18841 atccaccatt gccagcgcct ctttcacccg ctgctttact tcgctacgcg acacgccgag
    18901 catctttaac ccgtagccga cgttttcgcc cagcgacata tgcgggaaca gagcgtatga
    18961 ctgaaacacc atgcagatat cgcgctgctg aatggaacga tgcgtgacat cttcgccatc
    19021 aataaagatt tgcccttcac tcggtttttc cagcccggca accagacgca aaatggtggt
    19081 tttgccgcag ccggaagggc caagcagcgt caccatttgc ccttgtggga tggtgagatt
    19141 gatattgtcg ataaccgtat tactgccgaa tcgtttagtg acgttgcgca gttccacaaa
    19201 atttttctga ctcatagtgc gctccattac gcctggtttt tggctttaga acgggagata
    19261 cgtgcttctc cgatcagcca gtcaaagagg aaaataatcg ccagcatcac cacgatcaga
    19321 atcgaaccat aggcgatggc tacgccatat tcgccgtctt caacgcggtt aagaatgtag
    19381 gcggttgcca cgcgggtgtc cggcgtgacg agaaagacga tggcgctgac cgtcgtgatg
    19441 gcccgcacaa agctgtagat cagggccgag agaatggccg gacgcagcag cggcagtaaa
    19501 atgtgggtta tggttcgcaa ggaacccgcg cgcaggctga gtgaggcttc atccagcgat
    19561 ttgtcgatct gccccaggcc tgcaatcccg gcgcgaatac ccaccggaac gttacgcatc
    19621 accatcgaga taatcacaat tgccgccgtt ccggtgagat aaaccggagc gctgttaaag
    19681 gcaagaatat acgaaacgcc cgccaccgta cccggtacgg caaagcacag catggtggtg
    19741 aactcgatgg tctttttgcc tttgaattgt tgacgcacca ccacccaggc gatgagcagg
    19801 ccaaagatag cggtaatggg agcggcaatc ccggcgtaga gcagggtatc cagcagtgaa
    19861 ggccacgcgc cgtcgctcat tccctgacca aatagcttga taaagttatc cagcgtcagg
    19921 gtgtagtcca cgccccagtt caccgtgaag ctgccgtaga aaatgctgcc gtagagcagg
    19981 gcgttaaagg caatccatat cgcaagtatg gcgaccacgc tccacaccag ggtgaccgga
    20041 agcggctgga cgtcgccacg gtaggatttg ccggaaaccg tgacgtaaga gcgtttacca
    20101 atccacatat actgtatgca gaacaccagc agcgagaaca gcagcaggaa agcgccaagg
    20161 gtgctggcgg cctgataatc cagctgcgag ccggtaatat agaagtaaat ttgcgtcgcc
    20221 agtacgtcga agttaccgcc aagtaccagc gggttactga aatcagccag tgactgtacc
    20281 acgacgatta aaaaggcatt ggcgagcgcg ggcttcaaca acggcacaaa cacgccgtta
    20341 aaggtttgcc agcggctggc gcgcagggtg taggaggcct cttccagcga gggatgaatg
    20401 gttttaatgg ccccgtcgag gatcataaat gccattggcg tgaatgccag aacctgtgcc
    20461 agccagatcc cggtaaaacc gtacagccag ttggtgttgg tcagcccaaa ccactccacc
    20521 ataaattccg taacatagcc ggaacgcccc atcatgagcg tcacgcctaa gccgaccaca
    20581 aacggtggcg taacgatggg caaaatggaa aagatgcgac caataatggc gctgcgttta
    20641 gcgatgcgcg tggtataaat cgccagcacc aggccgaaga aagcacagcc tatccccacc
    20701 gctatcgaca agacgataga gttgacgatc acctgaacaa tatgtgcctg cgagagcacg
    20761 gtcataaacg ccagcggggc gagctctccc gcgtcgttgg tgaacatcgg gataaaaatg
    20821 gcgatgctcg gccagacgat aaaaatgcca atcagcgcca caatcgcaat cagtgagccg
    20881 atcacgaagc ggtcgccgcc cagccattcg aggcgggtta aggccagcgt cataatcgcg
    20941 cctagttcga caaacagcac gatggcggcg tagcctaaac cgcgtccagc cacggtggcg
    21001 ctgatgacga taaacgccat acagagcaat gcccagccgg catcgaaata atgacgacta
    21061 cgctgttcgc gcgtggcctg acgaaacggg cggaccagca acaggctcgg gagtaaatac
    21121 cacagccagc tgatattaaa atgcgaccag ccgtacgccc cgaggatctc atcactcgtg
    21181 gattccagca ggccataatc gaggctccag ctcggcaaca ggacaaatgc cagccagccc
    21241 agcaaaaccc aaaggaaaat cgcatcccgc tttttcacgg gatggagtgc aagtgtctgt
    21301 gacatagcag ttccggtatg gttggccggg gcaggcgtct gcgctgcacc cggcaaatgc
    21361 gcctaatgcg ggtttattta cccatcttca cttcgctgac ccatttatta atcaatgcct
    21421 tacgaacatc cgtggaacca tatttatcca tgtcgtagtt gattaatttc aggtcgtcga
    21481 gcttcagcga atttggcgac gtttcggcgg tggtattagt caggatctga taagacttcc
    21541 ctttcttcca cgccagttcc tgagcttctt ttgataatac ccagtccaca aacagtttgg
    21601 cgttgtcgag gttgcgcgcg cctttcagaa tgctgacgcc gccaatttcg tagcccgtac
    21661 cttcacacgg ggaaatcaac tccagcggcg cgccctgttc tttttccagc gagtagtcat
    21721 gcaggaagcc aataccaatc gccgtttcgc cacgggcggc gttacgtgcc ggggctatgc
    21781 ctgatttggt gtactgagag acgttggcgt tcagttgttt tagataatca aaggcctgat
    21841 cttcccccca cagctgcgca aaagtagcca gggcggtgta ggcagtaccg gaactttgcg
    21901 gatcggcaat ttgaatttcg cctttatatt ccggtttggt gagatctttc cagcacttcg
    21961 gcaccggcag gtttttctct ttcagacgat cggtgtttac gccaaaaccg aggatcccca
    22021 cataaacggc agaggacaga ttccctttca cttttgccgg atcgcggaat ttctccatga
    22081 tctgctcaag gttaggggat ttatacgcct gtagcaggcc catctcgcca gcctgcgatt
    22141 gcgggtctaa agtgccgccg taccagacgt cagcctgggg gtttttcttt tcagcatcga
    22201 cttttgccag ggtgcttccg gaaccgttgc ggataaacga ggtttttaca tcatatttct
    22261 cgccaaacgc tttggtttcg gcctcacaca tttcattggt ggcgctgcaa tagaccacca
    22321 gccggccttt tgcctgcgcg gcacccgtta aagtcgccag cgcaacggca gaagcaatca
    22381 gagtagaggt aaaggtcatt ttcattgttt tatccttcga gcgtgagtgg tggtgatgcc
    22441 cgccatcagt aacggcatca gcagtaaacc cataagtact gcggcgaccg agagtaaact
    22501 gaacattccc ggccagccgt agcgttcgat gaccagcgac agcggccagc ctgccagcgc
    22561 cgcgccaagg taagcgaaca ggccgagaaa tccggtgatt gaacccgccg cagctttgtg
    22621 cccacactcc accgccgcaa gtccaatcag catttgtggg ccaaagacga aaaagcccac
    22681 ggtaaaaaag cacactgcca gcagagcgta gtgatgaacc ggtgccagcc acagtgcggc
    22741 gactgacacc atcaacccca acgtgaacag caaaatcatt ggcgctcgtt gcccgctaaa
    22801 cagcaaatcc gatccccatc cggcaaataa cgcgccgagt aagccgccca cttcaaacag
    22861 cattaccgtg gcgttggcgc tgagcaggtt gacaccgtgg ctttccgtca gccagatatt
    22921 gccccagtcg ttaagcgcaa tccggatcac ataaaccagt acatatgaga cgcccagtag
    22981 ccagatcagc ggattttgca gcatggtggt gcgcaacatt tgccacagcc ccatcggcgg
    23041 actttgttgt tcctgacgta actccagcgg atcgtgacgc cagtgaccaa ctgtcggtaa
    23101 tccctcttcc tggggcgtgc ctttgagttg tagcgttaac cagatcccta atgccatgct
    23161 gattatcccc ggtgtcagca tcgcggcctg ccagccccac cagtgggcgg caaaggcgct
    23221 aatcagcgga ataatcgctc cgccaatgtt gatcgacata ttccagcagc cccaccagaa
    23281 accgcgctca ttgcgtgagt accagtgggt cagcaagcgg gcacacggtg gccatcccca
    23341 gccctgaaaa aaaccgttca gtgtccagac cacaagcaga agcgtaagtg attcgccaaa
    23401 ggcaaacacc acgttcaata acccggtggc gaacaagccg acgcccataa aaccgcgttg
    23461 tccgtggctg tcatgccaca gtccggcggt aaatttcgac aatccgtagc tgagataaaa
    23521 cagcgaaccc agcaggccaa tgtcgccttt atccagtccg aggtcggttt gtagcgccgg
    23581 aaggacgtaa ttcacgcttt ttcgcgtcag ataaaatgcg gcgtagccaa taatcatgca
    23641 catcagcaat cgcggacgta atgtccggta gcattgatta atttctctcg ctgaacgcgc
    23701 gtgcatggtt gtctccatgt tgctgactat atgaaaatga aagtgaaagg ggatgagaga
    23761 aaatccggga tacctaagac tttttcctgg ttatccggcg atttgttgca aaaatgtggg
    23821 caagttaaca attacccgcg tgccgtgctg tttttcgagc gttaagtcgc cgcccagggc
    23881 gctgacgcgc tcacgcatcc cctgaatgcc aaatccagcc attttcccgc tggcgatccc
    23941 cacaccgttg tcgctgactt ccagatgcaa tacctctccc tgctggcgca ggataatcgt
    24001 cacttcgctg gcttcggcgt gtttacagat gttattgagc aactcctgca ataaccgata
    24061 caaggtaaag cgcaccgttt cgttttctgg tgtggagttc agttgatagg caaactggca
    24121 atgaatgccg cgttcactaa aggcaaattc attaatcaga tgcagcaacg cttcgcgaaa
    24181 tgtcagttca tcaagcgcgg gaggccgcaa ctggcgcagc aattggcggg tggagagatg
    24241 aatgcggcgg gcgagctcat tgatttggct ggctgcggat tgaatttgcg ccggatcacg
    24301 cgcccgcttc accagttgcg actggatctg aatggcggtg atgttctgac cgatttcgtc
    24361 gtgcagctct cgcgccaggt tttttcgcgt gtcctcctcg gtgtggatca gcttttccgc
    24421 caatgcccgc cgcgctgcca gctcggcctc cagccgttgt cggtaatggt gcaggtttag
    24481 cgccagatgt tgctggcgac tgatggcaat gcccaggcca attcccagca aggcctgggt
    24541 cgcgaggaaa atctccagct ccaccaggtt actaaagccc acgccaatct ggcgggcgat
    24601 ggtgatcatc atgctgccca aaagcccgga gagtacgcca ccttgccagc caaatttcca
    24661 cgccatcacc acattgggca aaaagaccac gatcaacagc agacgttcaa tttcaggtga
    24721 cagcaccatc tgagtgccga taccaataat aaaaaacagg ctacaccaga tgattaaaga
    24781 ggtacgcaag gggggattat gcgtatcgag tcccaggagc tggtagcgat gctgctggcg
    24841 cagaaactca aagataagat agacaaacgg cgtcagtaat acgccgccgg taaatgaggt
    24901 caaacccggt agcattattg ggcctgacat aaaaggggag agcagggcgg tattcaacaa
    24961 tgaagctgcc gtgacagtgg ctaacagcaa cgtcagacgc tgccagtaaa gtggaaaacg
    25021 gtgccagaag ttttgcgcaa ggagggcagg aaacaggcta ataaccggcg ttgccagcat
    25081 gagataaccg ttcaatagct gttcactatg gagccagaac atcatcagca aaggggggag
    25141 caccaacgca ggccagtagc ggcgagagag caaaataaca agggcgagat aaactccctg
    25201 cgggagaaac aacaccgcct gttgaccgtt attggtcaga taaaagctga gcgtccacag
    25261 catcagccag ccggttcccc aggctagcac aataaacagc aaaatcaccc aatgacgaag
    25321 attgcgcgac attaatgccc cgccagcaga tggtggtcga gcgcaaaatg taccagttca
    25381 atggtgctat ggcagttcag ttttcccagc acattggcgc gatgcacatg taccgtttta
    25441 tgactgagtt cgagtttaaa ggcgatctct ttaacgctgt ctcctttgac cagtaactca
    25501 aacacttcac gttctcgtgg ggtgagaatt tccagcgcct gggcgggttg cccgccgccg
    25561 cgtaaagccc gaatcgcatc ggcacagaga taatgcccgc ccagcccgac cgagcgcacc
    25621 gcctgtacta actcttccgg cccacagcgt ttggttaaat acccgctggc accggcatcc
    25681 agcgcgcttt gcacaaaggt tggcgcgtca taaatgctaa gaataatggc gcgaaattgc
    25741 ggtttttgtg cacgcaggcg cttaagcaaa ctgaggccgt tttcgtccgg catggcaata
    25801 tcaatgacgg caacattgac gtcatcacgg attaatgccg accatgcctg cgcggcacta
    25861 ctgtactgac caatgacctc cagatcgtct tccagagaga gtaattgtgc gaagccagag
    25921 cgcaccacaa catggtcatc caccagcact acccgaatca tacttttctt ctcttattat
    25981 ctaattctcc tatcatgcat cacggcgcgg cgctggcaat caaccagcga cagatatgga
    26041 atggatgtaa caaaatggca ataatttgcc ggagcgcgca catcttatct ggcttgtgaa
    26101 ccggcgggat acgttcgcca aaaccgcaaa atgcgcaaac tcgctaacca ttgccgattt
    26161 ttttctaaaa acgctttgtt cttaacaaga ccttttaatt cctttatcaa actgacccgc
    26221 aacgaaatac tgctcccata acaagacagg ggagcagaca atcatggcaa tttcatcgcg
    26281 taacactctt cttgccgcac tggcattcat cgcttttcag gcgcaggcgg tgaacgtcac
    26341 cgtggcgtat caaacctcag ccgaaccggc gaaagtggct caggccgata acacctttgc
    26401 taaagaaagt ggagcaactg tggactggcg caagtttgac agcggagcca gcatcgtgcg
    26461 ggcgctggct tctggcgacg tgcaaatcgg caaccttggt tccagcccgt tagcggttgc
    26521 agccagccaa caggtgccga ttgaagtctt cttgctggcg tcaaaactgg gtaactccga
    26581 agcgctggtg gtaaagaaaa ctatcagcaa accggaagat ctgattggca aacgcatcgc
    26641 cgtaccgttt atctccacca cccactacag cctgctggcg gcgctgaaac actggggcat
    26701 taaacccggg caagtggaga ttgtgaacct gcagccgccc gcgattatcg ctgcctggca
    26761 gcggggagat attgatggtg cttatgtctg ggcaccggcg gttaacgccc tggaaaaaga
    26821 cggcaaggtg ttgaccgatt ctgaacaggt cgggcagtgg ggcgcgccaa cgctggacgt
    26881 ttgggtggtg cgcaaagatt ttgccgagaa acatcctgag gtcgtgaaag cgttcgctaa
    26941 aagcgccatc gatgctcagc aaccgtacat tgctacccca gacgtgtggc tgaaacagcc
    27001 ggaaaacatc agcaaactgg cgcgtttaag cggcgtgcct gaaggtgacg ttccggggct
    27061 ggtgaagggg aatacctatc tgacgccgca gcaacaaacg gcagaactga ccggaccggt
    27121 gaacaaagcg atcatcgaca ccgcgcagtt tttgaaagag cagggcaagg tcccggctgt
    27181 agcgaatgat tacagccagt acgttacctc gcgcttcgtg caataaaagg aggcgcagat
    27241 gctgcaaatc tctcatcttt acgccgatta tggcggcaaa ccggtactgg aagatatcaa
    27301 cctgacgctg gaaagcggcg agctactggt ggtgctgggg ccgtccggct gcggtaaaac
    27361 caccctgctg aatttgattg ccggttttgt gccttatcag catggcagca ttcaactggc
    27421 gggtaagcgt attgaggggc cgggagcaga gcgtggcgta gtttttcaga atgaagggct
    27481 actaccgtgg cgcaatgtac aggacaacgt ggcgttcggc ctgcaattgg caggtataga
    27541 gaaaatgcag cgactggaaa tcgcgcacca gatgctgaaa aaagtggggc tggaaggcgc
    27601 agaaaaacgc tacatctggc agctttccgg tggtcaacgt cagcgggtgg ggattgcccg
    27661 cgcgctggcg gcgaatcccc agctgttatt actcgacgaa ccgtttggtg cgctggacgc
    27721 cttcacccgc gaccagatgc aaaccctgct gctgaaactc tggcaggaga cgggcaagca
    27781 ggtgctgttg attacccacg atatagaaga agcggtgttt atggcgactg aactggttct
    27841 gctttcaccc ggccctggcc gcgtgctgga gcggctgccg ctcaactttg ctcgccgctt
    27901 tgttgcggga gagtcgagcc gcagcatcaa gtccgatcca caattcatcg ccatgcgcga
    27961 atatgtttta agccgcgtat ttgagcaacg ggaggcgttc tcatgagtgt gctcattaat
    28021 gaaaaactgc attcgcggcg gctgaaatgg cgctggccgc tctcgcgtca ggtgacctta
    28081 agcattggca cgttagcggt tttactcact gtatggtgga cggtggcggc gctgcaactg
    28141 attagcccgc tgtttttgcc gccgccgcaa caagtactgg caaaactgct caccattgcc
    28201 ggaccgcagg gatttatgga cgccacgctg tggcagcatc tggcagccag cctgacgcgc
    28261 attgtgctgg cgctactcgc ggcggtgttg atcggcattc cggtcgggat cgccatgggg
    28321 ctaagcccga cggtgcgcgg cattctggat ccgataatcg agctttatcg tccggtgccg
    28381 ccgctggctt atttgccgct gatggtgatc tggtttggta ttggtgaaac ctcgaagatc
    28441 ttactgatct atttagcgat ttttgcaccg gtggcgatgt cggcgctagc gggggtgaaa
    28501 agcgcgcagc aggttcgcat tcgtgctgcc cagtcgctgg gtgccagccg tgcgcaggtg
    28561 ctgtggtttg tcattttgcc cggcgcgctg ccggaaatcc tcaccggatt acgtattggt
    28621 ctgggtgtgg gctggtctac gctggtggcg gcggagctga ttgccgcgac gcgcggttta
    28681 ggatttatgg ttcagtcagc gggtgaattt ctcgcgactg acgtggtgct ggcggggatc
    28741 gcggtgattg cgattatcgc ctttctttta gaactgggtc tgcgcgcgtt acagcgccgc
    28801 ctgacgccct ggcatggaga agtacaatga gtgaacgtct gagcattacc ccgctggggc
    28861 cgtatatcgg agcacaaatt tcgggtgccg acctgacgcg cccgttaagc gataatcagt
    28921 ttgaacaact ttaccatgcg gtactgcgcc atcaggtggt gtttctgcgc gatcaagcca
    28981 ttacgccgca gcagcaacgc gcgctggccc agcgttttgg cgaactgcat attcaccctg
    29041 tttaccctca tgccgaaggg gttgacgaga tcatcgtgct ggatacccat aacgataatc
    29101 cgccagataa cgacaactgg cataccgatg tgacatttat tgaaacgcca cccgcagggg
    29161 cgattctggc agcgaaagag ttaccttcga ccggcggcga tacgctctgg accagcggta
    29221 ttgcggccta tgaggcgctc tctgttccct tccgccagct gctgagcggg ctgcgtgcgg
    29281 agcatgattt tcgtaaatcg ttcccggaat acaaataccg caaaaccgaa gaggaacatc
    29341 aacgctggcg cgaggcggtt gcgaaaaatc cgccgttgct acatccggtg gtgcgaacgc
    29401 atccggtgag cggtaaacag gcgctgtttg tgaatgaagg ctttactacg cgaattgttg
    29461 atgtcagcga gaaagagagc gaagccttgt taggcttttt attcgcccat atcactaaac
    29521 cggagtttca ggtgcgctgg cgctggcaac cgaatgatat tgcgatttgg gataaccgtg
    29581 tgactcagca ctatgccaat gccgattacc tgccacagcg gcggattatg catcgggcga
    29641 cgattcttgg ggataaaccg ttttatcggg cggggtaata cgagagtgga cggtcctctc
    29701 gcccccttgg ggagagggtt agggtgaggg ggcgttcacc gtgctttcaa caggttaact
    29761 ccccctttct gagaggaaac aaaattaacg cagaatcttc ttctcagcca aatccagcgc
    29821 aaagtagctg aaaatcagat ccgcacccgc acgcttaatc gaacctaagc tttcgagcac
    29881 gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg catactcacc
    29941 gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac gcacgatgtc
    30001 gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt catccagcag
    30061 tgactcacga atcgcctcac gacggttcat tgggttcatc tgatagcttt tgcggtcgcc
    30121 ttttaatgcg cttccggcag cttcacggaa cggaccataa aaggaggagg cgaacttggt
    30181 cgaatacgac ataatcgccg tatctttaaa gccagcagcg tccagcgcct ggcgaatcgc
    30241 ctgtacctgg ccatccatcg cggcggaagg ggcgatgaag tctgcacctg cagcagctgc
    30301 aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc catgctcgca
    30361 cagcacaccg cagtgaccgt gagaagtgta ttcacagaag caggtgtctg acatgacaat
    30421 catttctggc accgtctgct tgcagatgcg cgacatacgc gctaccagtc catcttcccg
    30481 ccaggcatcg ctgccggttt catcggtatg gtgagagatg ccgaaagtca tcacggaacg
    30541 aataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg gaatgcgcat
    30601 cacgccaggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa caaagatcgg
    30661 caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca gcgcaggaga
    30721 tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga tgtttgtgga
    30781 attaaggtgc acagtatacc tgaagcgggg ctggagtgtt ttacgaaagt tgtcacgatg
    30841 ttgacaagaa gagaatgaaa gagagaccag ggcatctttg taatagaaat agcaataact
    30901 tcatattcac tgaaaatatt ttttaatttt tatttatagg tgctggttat tatttttgtc
    30961 aatttggttg aactgtcaaa aattgaatta cttaaagttc attgcttatt gataaattat
    31021 tt
//
LOCUS       DAZKFZ010000039        30031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000039 DAZKFZ010000000
VERSION     DAZKFZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            92..1690
                     /gene="rtcR"
                     /locus_tag="ACHOQW_001597"
     CDS             92..1690
                     /gene="rtcR"
                     /locus_tag="ACHOQW_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP9572690.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1672..2430)
                     /locus_tag="ACHOQW_001598"
     CDS             complement(1672..2430)
                     /locus_tag="ACHOQW_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP9572691.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(2447..3277)
                     /gene="glpG"
                     /locus_tag="ACHOQW_001599"
     CDS             complement(2447..3277)
                     /gene="glpG"
                     /locus_tag="ACHOQW_001599"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP9572692.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3322..3648)
                     /gene="glpE"
                     /locus_tag="ACHOQW_001600"
     CDS             complement(3322..3648)
                     /gene="glpE"
                     /locus_tag="ACHOQW_001600"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP9572693.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3838..5343
                     /gene="glpD"
                     /locus_tag="ACHOQW_001601"
     CDS             3838..5343
                     /gene="glpD"
                     /locus_tag="ACHOQW_001601"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP9572694.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5558..6160)
                     /locus_tag="ACHOQW_001602"
     CDS             complement(5558..6160)
                     /locus_tag="ACHOQW_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572695.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(6160..7152)
                     /locus_tag="ACHOQW_001603"
     CDS             complement(6160..7152)
                     /locus_tag="ACHOQW_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9572696.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(7178..8686)
                     /locus_tag="ACHOQW_001604"
     CDS             complement(7178..8686)
                     /locus_tag="ACHOQW_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9572697.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(8811..11258)
                     /gene="glgP"
                     /locus_tag="ACHOQW_001605"
     CDS             complement(8811..11258)
                     /gene="glgP"
                     /locus_tag="ACHOQW_001605"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP9572698.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11277..12710)
                     /gene="glgA"
                     /locus_tag="ACHOQW_001606"
     CDS             complement(11277..12710)
                     /gene="glgA"
                     /locus_tag="ACHOQW_001606"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP9572699.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12710..14005)
                     /gene="glgC"
                     /locus_tag="ACHOQW_001607"
     CDS             complement(12710..14005)
                     /gene="glgC"
                     /locus_tag="ACHOQW_001607"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP9572700.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14023..15996)
                     /gene="glgX"
                     /locus_tag="ACHOQW_001608"
     CDS             complement(14023..15996)
                     /gene="glgX"
                     /locus_tag="ACHOQW_001608"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP9572701.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(15993..18179)
                     /gene="glgB"
                     /locus_tag="ACHOQW_001609"
     CDS             complement(15993..18179)
                     /gene="glgB"
                     /locus_tag="ACHOQW_001609"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP9572702.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18452..19555)
                     /gene="asd"
                     /locus_tag="ACHOQW_001610"
     CDS             complement(18452..19555)
                     /gene="asd"
                     /locus_tag="ACHOQW_001610"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9572703.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19747..20340
                     /gene="yhgN"
                     /locus_tag="ACHOQW_001611"
     CDS             19747..20340
                     /gene="yhgN"
                     /locus_tag="ACHOQW_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP9572704.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20386..21681)
                     /locus_tag="ACHOQW_001612"
     CDS             complement(20386..21681)
                     /locus_tag="ACHOQW_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9572705.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21588..23683)
                     /locus_tag="ACHOQW_001613"
                     /pseudo
     CDS             complement(21588..23683)
                     /locus_tag="ACHOQW_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(23823..25163)
                     /gene="gntU"
                     /locus_tag="ACHOQW_001614"
     CDS             complement(23823..25163)
                     /gene="gntU"
                     /locus_tag="ACHOQW_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9572706.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHLFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25167..25694)
                     /gene="gntK"
                     /locus_tag="ACHOQW_001615"
     CDS             complement(25167..25694)
                     /gene="gntK"
                     /locus_tag="ACHOQW_001615"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9572707.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(25833..26828)
                     /gene="gntR"
                     /locus_tag="ACHOQW_001616"
     CDS             complement(25833..26828)
                     /gene="gntR"
                     /locus_tag="ACHOQW_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP9572708.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(27052..27747)
                     /gene="yhhW"
                     /locus_tag="ACHOQW_001617"
     CDS             complement(27052..27747)
                     /gene="yhhW"
                     /locus_tag="ACHOQW_001617"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP9572709.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(27870..28907)
                     /gene="yhhX"
                     /locus_tag="ACHOQW_001618"
     CDS             complement(27870..28907)
                     /gene="yhhX"
                     /locus_tag="ACHOQW_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9572710.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            29241..29729
                     /gene="yhhY"
                     /locus_tag="ACHOQW_001619"
     CDS             29241..29729
                     /gene="yhhY"
                     /locus_tag="ACHOQW_001619"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9572711.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
     gene            29940..>30031
                     /locus_tag="ACHOQW_001620"
     CDS             29940..>30031
                     /locus_tag="ACHOQW_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572712.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTF"
BASE COUNT         7494 a         8077 c         7344 g         7116 t
ORIGIN      
        1 aaaagattat atctttacgt ccgtaaacgg agatttcccg caaagccaat ttaccgataa
       61 tgaaatatcg tcttttataa ggatatctaa gatgcgtaaa acagtggctt ttggctttgt
      121 cggaacagtg ctggattatg ccgggcgcgg cagtcagcgc tggtcgaaat ggcgtccgtc
      181 actctgtata tgccagcaag aatcgttagt catcgatcga ctggaattat tgcatgacgc
      241 ccgctcgcgc tcgctatttg aaacgcttaa acgcgatatc gccagcgttt cgccagaaac
      301 cgaagtggtg agcgttgaga ttgaactgca taacccgtgg gatttcgaag aggtttacgc
      361 ctgcctgcat gatttcgccc gtggttacga gtttcagcca gaaaaagaag actatttaat
      421 tcacatcacc accggcaccc acgtcgcgca gatttgctgg tttctgctgg ctgaagcgcg
      481 ttacctgccc gcccggttga tacaatcttc acctccgcgc aaaaaagaac agccgcgcgg
      541 cgcaggtgaa gtgacgatta tcgatctcga tttaagcctt tataacgcca tcgccagccg
      601 ctttgccgag gaacgccagc aaacgcttga ttttcttaag tccggtattg ccacgcgtaa
      661 cccccacttc aaccgcatga ttgagcagat tgaaaaagta gcgattaaat cccgcgcacc
      721 gatcctgcta aacggcccaa ccggtgcggg caagtcattt ctggcgcgac gtatatttga
      781 attaaaacag gcgcggcatc agtttagcgg cgcgtttgtg gaagtgaact gcgcaaccct
      841 gcgcggcgat accgccatgt cgacgctgtt tggtcatgtg aaaggcgcgt ttaccggggc
      901 acgggaatct cgtgaaggat tattacgcag cgccaacggc ggaatgttgt ttcttgatga
      961 gattggcgaa ctgggcgcag acgaacaggc aatgctgctg aaagccattg aagaaaaaac
     1021 cttttatccg tttggcagcg atcgccaggt gagcagtgat tttcagctta tcgccggaac
     1081 ggtgcgtgat ttgcgccagc tggttgccga tggcaaattc cgcgaagatc tatacgcgcg
     1141 gatcaatctc tggaccttca ccctgccggg gctacgccag cgtcaggaag atattgaacc
     1201 taacctggat tatgaagtgg agcgccacgc ttcactcacc ggcgacagcg tgcgttttaa
     1261 caccgaagcg cggcgcgcct ggctggcctt tgcgacatct ccccaggcaa catggcgcgg
     1321 taactttcgc gagctttctg ccagcgtcac gcgaatggca acctttgcct ctagcggacg
     1381 cattactctg gacgtggttg aagatgagat aaaccgtctg cgctataact ggcaggagag
     1441 ccgcccctcc gcgcttacgg cgttgctggg cgcagaggca gaaaacatcg atctcttcga
     1501 ccgtatgcaa ctggaacacg ttatcgcaat ctgccgccag gcaaagtcgc tttccgcagc
     1561 cggacgccag ctttttgacg tttcgcgcca gggcaaagcc agcgtcaatg acgcggatcg
     1621 gctacgcaaa tacctggcgc gttttggtct gacgtgggaa gccgtgcagg atcagcacag
     1681 ctccagttga atatggtggt ccgtcagcac ctgcatcacg cttactggcg gcggggtgtc
     1741 ggtgtagacg gcatctacca tgctgatgct acccatattg accatcgcgt tacggccaaa
     1801 tttcgagtga tcgacaacca gcataacgtg gcgcgagttc tcaataatgg cgcgtttggt
     1861 gcgaacttcg tggtaatcga actccagcag cgagccgtcg ctatcgatgc cgcttatccc
     1921 cagaatgccg aaatcaaggc ggaactggga gataaaatcg agcgtcgctt cgccaatgat
     1981 cccgccatcg cggctgcgta attcgccacc ggcgagaata atacggaaat cctcttttac
     2041 catcagcgtg ttagcaacgt taagattgtt ggtgacaatg cgcaaattgc tgtgattgag
     2101 cagcgcgtgc gccaccgctt ccggcgtggt gccgatatcg ataaacagcg tcgagccatt
     2161 ggggatttgc tccgccactt tgcgggcgat gcgctctttt tcttcggtct gggtggcttt
     2221 acgatcgtgc cacggcgtgt taaccgaact ggaaggcagc gccgcaccgc catgatggcg
     2281 caggatcagg ttttgctccg ccagctcatt gaggtcgcgg cgaatagtct gcgggctgac
     2341 ggagaagtgc tccaccagct cctcggtact gacataaccc tgctgtttaa ccagttcgat
     2401 aataccgttg tgacgttgtg tttgtttcat ttctaaatcc ctggaattat tttcgttttc
     2461 gcgcattgag cgaatcaaca aaagccatcg ctaaacccac ggctaacccg gcgatgtgtg
     2521 ctccgttcgc catcgacatc ccaaacaaat caaaccatcc ggcgacaatc cagatcagcg
     2581 caaagataat taacccacgt tgcaggtaaa tgccactttg cggatcgcgt tcgccacgta
     2641 gccagacgta gcccatcagc gcatacacca cgccagaaag cccgccaaac cacggcccgc
     2701 tgaatttttg ctgcacatag ccgcttaaca gggcgctgat aagcgtaatg acaattagct
     2761 taccgctacc gaggcgtttt tccaccgcac cgccgagata ccaccaccag agcaggttaa
     2821 agaggatatg catcagcgag aagtgcatta acgcgtgggt gaagtaacgc cagaactcaa
     2881 atttcagtgc tggatcgaat ggccaagcca gccataacat cacttcctga tcgccgagaa
     2941 tttgcatggc aataaacacc accacgcagg cgatcatcat cacccaggtc accggacctg
     3001 cgcgttcacg caaggcggca aagaaaggat aacggcgata atgcaggcca ctgccggtat
     3061 ggcctgcctg ccagctcgcc gccagataac gcggatctgc cgggttttcg agaaaacgcg
     3121 ccagctccgc ccgtacgcgc tcggcctggg actcatccgc cagccagaca tcgctttggt
     3181 tatgttgttg aatcgtgagg ataacaccct gcgtcgccat gtaatcaaca aacgcctgcg
     3241 ccacgcgggg gttagcaaaa gaggtaatca tcaacatcgt tgctgtcgct tattccacac
     3301 aaaaggggac agtataaagc gttacgcgcc gtacgccacc tctgcgggaa actgacgttg
     3361 ccaggcttca aagccgccgt caatgctata gaccacatcg tagccctgtt gcagcagata
     3421 ctgcgccgcg cctttgctgc tattgccgtg ataacacatc accatcaccg gagtgtcaaa
     3481 gtcgttatta cgcataaaag cgcccagcgt gtcgttggtt aaatggaaag cctgcaccgc
     3541 atgtcccatt gcgaaactct gtggatcgcg aatatcgacc agcaccgcct ctttttcctg
     3601 caacttctgg tgcgcgtcgg caacgttaat acattcgaac tgatccatgc gtctctcttt
     3661 ctttacaaac aagtgggcaa atttaccgca cagtttacgt cgaagcggca gataaacgcc
     3721 ataatgttat acatatcact ctaaaatgtt ttttcaatgt tacctaaagc gcgattcttt
     3781 gctaatatgt tcgataacga acatttatga actttaacga aagtgaatga gggcagcatg
     3841 gaaaccaaag atctgattgt gataggtggc ggcatcaatg gtgctggtat cgcggcagac
     3901 gccgctggac gcggtttatc cgtgctgatg ctggaggcgc aggatctcgc ttgcgcgacc
     3961 tcttccgcca gttcaaaact cattcacggt ggcctgcgct accttgagca ctatgaattc
     4021 cgcctggtca gcgaggcgct ggctgaacgt gaagtgctgc tgaaaatggc cccgcatatc
     4081 gccttcccga tgcgttttcg cctgccacat cgtccgcatc tgcgcccggc gtggatgatt
     4141 cgcattggtc tgtttatgta cgatcatctg ggtaaacgca ccagcctgcc gggatcaact
     4201 ggtttgcgtt ttggcgcaaa ttcagtgtta aaaccggaaa ttaagcgcgg attcgaatat
     4261 tccgactgtt gggtagacga tgcccgtctg gtgctcgcca acgcccagat ggtggtgcgt
     4321 aaaggcggcg aagtgcttac tcgtactcgt gctacttctg ctcgtcgcga aaacggcctg
     4381 tggattgtgg aagcggaaga tatcgatacc ggcaaaaaat atagctggca agcgcgcggc
     4441 ctggttaacg ccaccggccc gtgggtgaaa cagttcttcg acgacgggat gcatctgcct
     4501 tcgccttatg gcattcgcct gatcaaaggc agccatattg tggtgccgcg cgtgcatacc
     4561 caaaagcagg cctacattct gcaaaacgaa gataaacgta ttgtgttcgt gatcccgtgg
     4621 atggacgagt tttccatcat cggcaccacc gatgtggaat acaaaggcga tccgaaagca
     4681 gtgaagattg aagagagtga aatcaattac ctgctgaaag tgtataacgc gcactttaaa
     4741 aaacagctta gccgtgacga tatcgtctgg acctactccg gtgtgcgtcc gctgtgtgat
     4801 gatgagtccg actcgccgca ggctattacc cgtgattaca cccttgatat tcatgatgaa
     4861 aatggcaaag caccgctgct gtcggtattc ggcggtaagc tgaccaccta ccgaaaactg
     4921 gcggaacatg cgctggaaaa actaacgccg tattatcagg gtattggccc ggcatggacg
     4981 aaagagagcg tgctaccggg tggcgccatc gagggcgacc gcgacgatta tgccgctcgc
     5041 ttacgccgcc gttatccgtt cctgaccgaa tcgctggcac gtcattacgc gcgtacttat
     5101 ggcagcaaca gcgagctgct gctcggcaat gcgggagcgg taagcgatct tggggaagat
     5161 ttcggtcatg agttctacga aacggagctg aaatatctgg tcgaccacga gtgggtccgc
     5221 cgcgccgacg atgccctgtg gcgtcgcaca aaacaaggca tgtggctgaa tgcggatcaa
     5281 caatctcgtg tgagtcagtg gctggtggaa tatacgcagc agaagttgtc gctggcgtcg
     5341 taaattaacg taaggtgatc aggtcagatt tcaatctggc ctgagactga tgacaaacac
     5401 aaaattgcct gatgcgctac gcttatcagg cctacgtggt ttatgcaata tgttgaattt
     5461 gcatgatttt gtaggcctga taaggcgttc acgccgcatc aggcatgaac aaagcgccac
     5521 gttgtcaaca atctgaccta ctttttgatt cagggaatta ccgcaaagcc cacgccatca
     5581 tacccaacgt aacaaaactt aagcacgttc cccactcact ccacttccgt atctggcttc
     5641 tatttttgct acgccactgg caataatatc ccaacgcaaa accaagctgt gcacttatcg
     5701 gaccgacaaa gtcgatccac tgttgagacc aaaatggcga aaaattcagt gggagccaga
     5761 caactgtcag aaagaaactg cgcatatttt ccagcaaagc aatttgcgct ggcgcgacat
     5821 aaaaaatgat attccacagg agcaaccaga aataaactgg tgcaaatacc agccaacaat
     5881 gcagtctgct tttcaattca ggtggcgttc tgcgcgcaaa ttgccaacca acgcccacca
     5941 ttgcacagaa gaaaagcgca tattcagccg ataaccatag ttgtaattgc agaagggaaa
     6001 tacgcctgtg catgagcggg attgagaaca gtatttctcc cagaaaactc agcgtgatta
     6061 cacaggcagc cagcgcaacc accgtcggcc acaccaacaa cggtacaata acaaagccga
     6121 gaaataacgg ataaatataa agtctgatta tattcatcat tattgtcctg atgattcggg
     6181 ggatgcttca tacagcggca cataccccac atcctctacc aactgttgcc cctgctgact
     6241 tataaaccag tcaacaaatt tctgcgtttc cggcgtggga ttttcacgcg tcaccatata
     6301 ggcatccaca atgtatggat aagtgccgtt gcggatattt tcctcggtag gcgcaacgcc
     6361 atttatagcc aaaagtttaa tctgcttgcg cattttgagc atattgtcat tcatacgagt
     6421 gacgtaatag tggaaggtgt acccaatgga agatggcgtg ttttgatagt cggcaacttc
     6481 agtaattaaa ccgcccataa gatcaatcac agtggatttt ttagctggca gcattggggt
     6541 gtcttgcata accaacccct tcataatcgt ttggcttcca gaatcttcag ggcgctgcca
     6601 gacttttata tgttcgtcac taccacctac tttattccag cggctagttt tgccgctaaa
     6661 aatatcttta acctggtgct cagagagaga atttaccggg ttattaatat caacgataaa
     6721 gacaaaggct tcgcgggcga aagcggtata tttcagttta acacccgctt cttctgcacg
     6781 ttttttctgt ccttccgacg gttgtgcaac aaaaataacc gtcgcggaac ctttaatcag
     6841 actatcgtag gcttcagctg ttcgggtttg ctgcaaacca tacgcctgcc aggccagcga
     6901 ggttcgatcc ttatcttttg gctcgggtac taatttataa tacgcggagg catacagcgg
     6961 gtataaggct gtagcgccgt acactctcgg ccatcttgac caatcatcgc tgtaacgtaa
     7021 tgcaggttgc ccccgcagag acgtcagtcc atggctctct tcttccctga cgctgttata
     7081 gtacccttcc cggtcgacac atcccgccag taacgccgtg cctaatagta aaacaccggt
     7141 gaatttattc atctcgctga ttcctttctc tcgagcgtta ttccattgtt agatacagcg
     7201 gcacatatcc cacatcttct accagactct gcccctgcgg cgttaaaaac cattcgacca
     7261 gtttttgtgt ttctgacgtt gtattttctc tcgtcaccat aaatgcatcg acgatgtacg
     7321 catatttgcc gttgcgaatg ttttccgccg tcggtgtaat accgttaatc gctagcaatc
     7381 ttatattttt atcagcattc atttgcgtcg cgtaatagcg gaaggtatag cctattgcgt
     7441 tgtttgtatt acggtattcg gcaacgactt taatcattcc ctccatcacg cttgccactt
     7501 ccgtttcctg cggcgagatc atgcggactt ttttcatgac ctgtgattgc atcactgtct
     7561 ggctgccaga gtcttccggg cgctgccagg tctggatctc ctgatcgtta ccgccaacag
     7621 tacgccaatt ggtaattgca ccactgaaaa tgtcacgcac ctgttgttca gtcagggaat
     7681 taaccggatt atccgcattg acgatgaaaa caaatgcttc acgggcaaat ggcgtgtata
     7741 gcaaagtgac gcccgattcc tcagcgcgtt ttttctgccc gccggagggt tgcgccacaa
     7801 aaataatatc ggcatcacct ttaacaatcc ggttatatgc atcgggggta cgagagttct
     7861 ccagatattc ccaaacgtga aaatcctctg gtattacact taatgcataa aatgcagaag
     7921 cataaattgg gtacgcagcc gtggcgccat cgattcgcgg ccagttttgc ctaaactgaa
     7981 tttgcggttt gccacgcagc ggcgtcagtc gattattgag tttatcgggt cgccaggccc
     8041 tgatatcaat attttcgcgt acaccgacac caggattttg ctgcttatat aaatgggcct
     8101 gatacccggc gacaatacca agtaagagaa gtagaaacag gagtaagtta cggcaacgta
     8161 gtccggacgt gccggataaa ggatatctgg tcggccaggc gtatcccagc gaaaaactga
     8221 tatgcgcccc aacgggaata atgaccatcc accagtactc accacccact aatgagacta
     8281 aaaaaacgcc caaccaggga agcataaggt ttgccaacca accagctccc atctggcgaa
     8341 attgataatc tgccagatgt agcgtgatta tccagccaac cagggttaat agcgcgggta
     8401 agagaaaaaa gcattgccgc cagacggcta tttctctgtt ttctggcgcg ctatcaccgg
     8461 cccacacgcg accagagaaa aatattatta tcgcccccag cgtcagcata aagacgttga
     8521 gtagaagata aattgttggc gtgataagga tattacaaac ttcaataata ccggtaagcc
     8581 cgacacccag catggcaaca accgctgcca aaaattgcgc cagtatgggg atgccgaaaa
     8641 aagtcattat cagcgaggtc aaaatccatt ttctatttgg catcattctg tccattcttc
     8701 ctgaatgacg gaattatact ccccgagaac cctcgccccc tctggacact tttccgaaat
     8761 aaaggcgaaa aaaacgggac cttgtggccc cgttctgttt tttagcgaac ttacaatctc
     8821 accggatcga tatgccagat atgatcagcg tactctttga tagtacggtc agaagagaag
     8881 tagcccatat tggcaatgtt cagcatcgct tttgcggtcc actcttcctg aagctcgtag
     8941 agttcatcga ctttatcctg acaatcgaca tagctgcgat aatccgccag tacctggtag
     9001 tgatcgccga agttgatcag cgaatcaacc agatcgcgat agcgacccgg atcttccgga
     9061 ctgaatacac cgctgccgat ttgcgtcagc acctgatgca gctcctcatc tttctcgtag
     9121 tattcacgcg gtttgtagcc ctgacgacgc agttcttcca cttcttccgc tgtgttacca
     9181 aaaataaaga tattgtcagc accgacatga tccagcatct cgacattcgc accgtccaac
     9241 gtaccgatag tcagcgcacc gttaagcgca aacttcatgt tactagtgcc ggaagcttcc
     9301 gtccctgcca gcgaaatctg ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg
     9361 ctaacgctgt agttcgggat gaacacgact ttcagcttat cgccaatctg cggatcgttg
     9421 ttgatcactt tcgctacgtc attgatcaaa tggataatat gcttcgccat gtaataggcc
     9481 gaagccgcct taccgccaaa aatattcacg cgcggtaccc acttcgcatc caggtcggcc
     9541 ttgatgcggt tatagcgggt aatcacatgc aacacattca tcaattgacg tttgtattcg
     9601 tgaatacgtt tgatttgcac atcgaacaac gcctttggat tcaccaccac attcagctgc
     9661 tgggcgatat actccgccag acgcttttta ttctccagct tcgcctgatg cacagcgtga
     9721 ttaaccattg ggaaatcaca gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc
     9781 cagttacggc ccaggtgttc gtccagcacg gctgaaagcg atgggttcgc taccgccagc
     9841 cagcgacgcg gcgtcacacc gttggtgacg ttggtgaaac gacccgggaa gattttcgca
     9901 aagtcggcaa acaacgattg caccatcaga ttagagtgca gttccgatac accgttaact
     9961 ttgtggctca caacaaccgc cagccaggcc atacgcacac gacgaccgtt ggattcatca
    10021 atgatcgacg cccgtcccag cagatcggta tcgttcggat actgttcctg caaggttttc
    10081 aggaaatagt cgttgatttc aaagatgatc tgcaggtgac gcggcagaat tttacccagc
    10141 atatcaaccg gccaggtttc cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag
    10201 acctgacaac acacctcaaa cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga
    10261 cgcatcatct caggaatcga cagtaccgga tgggtatcat tgagatgaat cgcgatttta
    10321 tccgccaggt tatcgtaggt tttatgcaac tgataatggc ggcttaaaat gtcctgaatg
    10381 gtcgaggaaa ccaggaagta ttcctgacgc aggcgcagct cacgcccgga gtaggtggag
    10441 tcatccggat acagtacgcg agatacgttc tcggagtggt ttttatcttc cactgccgcg
    10501 aagtagtcac cctggttgaa tttaccgagg ttaatttcgc tactggcttg cgcactccac
    10561 aaacgcagcg tgttggtcgc gtcggtgtcg taaccaggga ttatctgatc gtaagcgact
    10621 cccagaatct cttcggtttc aatccagcgc gtttttttac cttcctgctg aatgcgacca
    10681 ccaaaacgga ctttatagcg cgtgttgtgg cgtttgaatt cccacgggtt accatattcc
    10741 agccagtagt caggcgactc tttctggcta ccgttaacga tgttctgctt gaacataccg
    10801 tagtcatagc ggatgccgta accgcgcccc ggcaacccta acgtcgccag agaatcgagg
    10861 aagcaagccg ccagacgtcc caggccaccg ttaccgaggc ccgggtcatt ttcttcatca
    10921 atcagctctt cgagatttaa ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt
    10981 cctagcgaca acatggcgtt ggagagcgta cggccaatca aaaactccat cgacaggtag
    11041 taaacctgac gagtttcttg cgacaactgg gcacggtttg aacgtaacca gcgctccacg
    11101 agacgatcgc gcacagcaaa taacgttgcg ttcagccatt catgtttatt ggcgacgacc
    11161 gggtcctttc caatcgtaaa catcagcttg taagcgatag agtgcttaag agcttctacg
    11221 ctaagcgtgg gcgatgaata tgtaaacgga gcattcatat aggcgtttcc tgaaaactat
    11281 ttcaagcgat agtaaagctc acggtacgac ttcgccgcga cctgccagct aaaatccatt
    11341 gccatagcct gacgttgcac aaaccgccac agagaaggac gggaccacag tacaaaagca
    11401 cgtcgaatag cccgtaacag cgaccaggca ttactatctt caaagacaaa cccactggcg
    11461 acgccatctg caaggttctc aagagaacag tcagaaaccg tatcagcaag cccaccggtg
    11521 cgccgcacta acggcagcgt accgtacttc aatccataaa gttgcgttaa gccgcacggt
    11581 tcaaaacggc tgggcaccag aatgacgtcc gcaccgccca taatgcgatg cgaaaatgct
    11641 tcgtgatagc caatctgaac gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa
    11701 ccttcctgca gcaccggatc gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga
    11761 agacccggta aggcctccag caccaggtcg aggcctttct ggctggtcag acggctcacc
    11821 actgcaaaaa gcggcacttt atcgtcaacc ttaagcccca ttgcgatttg taactggcgc
    11881 ttattttccg ctttatcttc caacgtatcg cgggtgtaac gcgaggccaa tagtaagtcc
    11941 gtctctggac tccagatttt ctcgtccacg ccgttcagta cgccggaaag acgcccttca
    12001 cgatgacgct gttgcaacaa accttccata ccgtaggcaa actgcggttc ggtgatctcg
    12061 cgagcgtagg ttggactgac cgccgtaatg tgatcggcat agtacagacc ggccttcagg
    12121 aaagagattt gtccgttgaa ttccagccca tgaatattaa agaatgacca tggcaattgg
    12181 atgtcattca tgtgatgtgc ataaaacatg ccttgatagg ccaggttgtg cacagtaaac
    12241 accgacttcg ccggacgccc gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag
    12301 tcgtgcgcat gcaccacatc aggacgccag aatgggtcaa gcccgctggc catttctgcc
    12361 ccaacccacc ccagcagcgc aaaacgcaat acgttgtcgg tataggcaaa taagttggta
    12421 tcgtgatacg ggcttcccgg acgatcatag agatgcggcg cgtcaatcag gtatatgcca
    12481 accccgttgt aatgaccgaa caacagcgtg atatgcccgg cgaaggtatc acgacgggat
    12541 actacctgcg catcggtcac gccacggcga atatcgggaa atgcaggcaa cagtacgcga
    12601 gcgtcaacgc cgtctgcgat ttgtgctgcg ggtaatgccc caataacatc agccagaccg
    12661 ccggttttaa gcagcgggaa catctctgaa catacatgta aaacctgcat tatcgctcct
    12721 gtttatgccc taacttccgt agcatttcgc gcgttaccag cacgatgcct tcttctgaac
    12781 gatagaaacg acgtgcatct tcctctgcgt tttcaccaat caccatgcct tccggaataa
    12841 cacaagcacg atcgatgacg cagcggcgca gacggcacga gcgacctacc catacttccg
    12901 gtaacaatac ggcggaatca atgttgcaga atgaattcac gcgaacgcgc gagaacagaa
    12961 cggactgcac caccaccgaa ccggagatca cacaaccgcc ggaaaccagt gagttaaggg
    13021 tcatcccgtg gctaccggag cgatcctgca cgaatttcgc tggcggtaat gattcattgt
    13081 aggtgcgaat tggccaattg cgatcgtaca tatccagttc cggcaccaca gaggccagat
    13141 cgaggttcgc tttccagtaa gcttccagcg tacccacatc gcgccagtac ggctcggcat
    13201 ccggatcgga ttgtacgcaa gagagcggga acgggtgcgc ataggccaga ccggcttcgg
    13261 tgatcttggg aatcaaatct ttgccaaagt cgtggctgga gttctcatcg cgatcgtctt
    13321 cttccagcag ttcatacaga tagtcggcgt caaagacgta gatacccata ctcgccagag
    13381 atttgctcgg atcgttcggc attgacggcg ggttagcagg tttttcaacg aattcgataa
    13441 ttttatcgtt ctcatcaacc gccataacgc caaatgcgga ggcttcttca atcggtactg
    13501 gcatacaagc aacggtgcaa cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt
    13561 agtcttgctt gtagatatgg tcgcccgcca ggatcaccac gtattccgct ttataacggc
    13621 gaataatatc gaggttttgg gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt
    13681 tcattctctg ctgtgctggc agcagatcga caaactcgtt catttcttca ttgaagaatg
    13741 accagccgcg ctgaatgtgc tgcaccagag tgtgggactg gtactgggtg atcacgccca
    13801 tacgacggat cccggagttg atgcagttag acagcgcaaa gtcgataatg cggaacttac
    13861 cgccgaagtg tacggccggt tttgctcgct tattggttaa atccttcagg cgggtaccac
    13921 gtcctcccgc cagtatcagg gcaacagatt tcaatggcag ctggcgcgcc aacattaagt
    13981 gatcgttctt ctctaaacta accatgacta actccttttt tatcatctct ggaacacaca
    14041 caatccgtgt gcaggtccct gccagacagc cgtaatcact gggttatcct ctccagcgaa
    14101 tgggggaatg gcgtgccact ccccagcagg taaaacaatc tctgttacct caagcgtggc
    14161 gttaattgcg atcaaaaagc gatccgagag cagaatttgc agctgtttcg gcccgttttg
    14221 ccactcatcc gtgcttaaag gttgagcata tcgatttagc caacggacat tgccgtcgcc
    14281 ttcttcccac cagcgattct ccaccaaagc gggaatacgc ttgcgcagat ggattaacgc
    14341 ggcggtaaat gcggttaaac cactgcttgc ctgcgaccag tccaaccagg ttaattggtt
    14401 atcctgacag taggcattgt tattgccatg ctggctgtga ccatgttcgt caccggccag
    14461 taacatcggc gtaccctggg agagcaacaa cgttgttaac agggcgtgaa tgctgtcgcg
    14521 ccgccgttca accaggtcaa gggtaccgcc taccccttct ttaccatgat tgttactgta
    14581 attgttgttg gtcccgtcgc gattttcttc tccgtttgct tcattgtgtt tatggttgaa
    14641 acaaacgcag tcgcgaagcg taaaaccgtc atgcgcggtg acgagattaa tcgcggcact
    14701 cggcagacga ccattacgtt taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc
    14761 ccccagaggc aaatcataat gtagccagaa acgacgggca gcatcgcgga aatgatcgtt
    14821 ccactcggca aacagcggcg ggaaatttcc cacctgataa ccaccaggag cgatatccca
    14881 cggttcagca attaacttca cctgcgagag caccgggcag ttctggatag cggtaaacaa
    14941 cggcgcatcc tgacggaact ctggcgtacg gcccatgact gccgccagat caaagcggaa
    15001 accatcgacg tggcaggttt ctacccaata acgcaggcag gcgctggcat aatccaccac
    15061 cgccgggtga ctcaaattga gcgtgttgcc acaaccggtc cagttgtgat aatcgccgtc
    15121 ttctcttatc caataatagc tacggttgtc gatcccacgc agcgagaata acgggccgtc
    15181 gaggtccagt tccgcactat ggttgagcac gatatcaaga atgacttcga tacccgcttt
    15241 atgcagtgct ttgattgcat cgcgaaactc atgcagcgcc gtttctggcg agcaggcata
    15301 cgccggatgc agcgcaaaca tcgccaccgg gttgtaaccc cagtagttac ttagccccat
    15361 gcgttgcaga cgtggttcac tggcaaactg cgccactggc agcagttcca gcgcggtaat
    15421 gcccaattgt ttcaaatagt tgatcatcac cggatgcccg agggctttat aagtgccacg
    15481 gatctcgacc gggatctccg ggtgcaggta cgttaatcct ttaacatggg cttcataaat
    15541 gatggtgctg ccccacggcg tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac
    15601 caccactacg catttcggcg caatggcggc gttgtcgcga tagtcaggtt cattatgacc
    15661 ggcgtgcagc agcgggttat ctttaaactc cccgtcaatt tgccgcgcgc aaggatcaat
    15721 caacaacttc gccgggttaa agcgatgccc ctcggcgggt tgccaggggc catgaacgcg
    15781 ataaccataa cgcaaacccg ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact
    15841 gtgccctggc aagtcatagc gatgttcctg gccattggcg tcaaagacac acagttctac
    15901 ccgctcggca tgagcggaga aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc
    15961 gaggggagcg ggtttgccaa tggcgagttg tgtcattctg cctcccgaac cagccagata
    16021 gtggccagcg gtggtagcgt caggcttagt gaatgctgac gaccgtggct ggcaatctca
    16081 tcgctgtgta ccgtgccgcc attacctgca ttactgccgt gatagtgcat ggaatcggta
    16141 ttgaggatct cacgccattt gcctggctga ttaatgccga agcgataatc atgacgcggc
    16201 accggcgtaa agttactggc aacgatgatt tcgttaccct ctttatcgcg acgcacaaag
    16261 atcagcaccg agcgttcttt gtcatccacc accagccatt caaagccgta cgggtcaaaa
    16321 tccagttcat gcatcgcttt atggtggcgg taggtgaggt tcagatcgcg caccagacgc
    16381 tggacaccgt ggtgccagtt atcgccgcct tccaacagat gccagtcgag gctggcgtca
    16441 tggttccact cgcggccctg ggcaaattcg ttccccatga acagcagttt cttgcccgga
    16501 aatgcccaca tccagccata gtaagcgcgc aggttagcga atttctgcca tgcgtcgccc
    16561 ggcatgcggt cgagaatcga ttttttaccg tggaccactt catcatgcga caacggcagg
    16621 acgaagtttt cagtgtagtt gtagagcatc ccgaaggtca gtttatcgtg atgatactga
    16681 cgataaatcg ggtcgagctt catgtagtcc agggtgtcat gcatccagcc gaggttccac
    16741 ttgtaccaga agcccagacc gcccatatcc tgcggacgag aaacgccagg gaaatcggta
    16801 gactcctcag ccattgtcac cgcaccggaa acctgctcac caagaatacg gttggtatta
    16861 cgcaagaatt caatcgcttc aagattctcg cgcccgccaa attcgttcgg gatccactcc
    16921 ccctctttac ggctgtagtc acgataaatc atcgacgcca ccgcatcgac gcgcagcgca
    16981 tcaataccaa aacgttcaat ccagtaaagc gcgttaccga cgaggaagtt actgacttca
    17041 cggcgaccat agttgtaaat cagcgtgttc cagtcctgat gatagccttc gcgcggatcg
    17101 ctgtgttcat acaagttcgt accatcaaat tcggcaagcg caaagtcatc agtcgggaag
    17161 tggcctggca cccagtcgag aatcacgttc agaccagctg cgtgtgcagc atcaatgaaa
    17221 taacggaagt cgtcgcgagt accaaaacgg cgggttggtg catacaggcc ggttggctga
    17281 taaccccaac tgccatcgaa gggatgctcg ttaaggggca gtagttcgag gtgggtaaag
    17341 cccatccatt tagcataagg caccagttga tcggccagct cgcggtagct caaccagaaa
    17401 ttgttgtcgg tgtggcgacg ccaggagccc aggtgaactt cataaataga gattggcgca
    17461 tcaaactgat tcgctttttt gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa
    17521 ataagagacg cggtttccgg gcgcatttgc gcttcgaagg cataagggtc ggacttcaga
    17581 cgcaagttgc cattggcatc aatcatctcg tatttataga gctgaccgtt atgcgcccca
    17641 gggataaaca gttcccagat gccgctctct ttacgcaggc gcatcgggtg acggcgaccg
    17701 tcccagtagt tgaattgccc aaccaccgag acccgacggg cgtttggagc ccagacagag
    17761 aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg cgcctaaagt ttcatacggg
    17821 cgcaggtgag taccttcaga taatagccag gcatccattt cctggattag cggaccaaaa
    17881 cggtaaggat catcaatcag gttttgctgc ccatgccaga caacagccaa ctgatagcgg
    17941 aaaaaattct tacgtcgcgg aatgacgcca ctaaagaatc cccgtgagtc gagacactcc
    18001 agttttgcga gtttgcgccc ggttttcggt tcaatcaccc acacatcggt agcgtcgggt
    18061 aaaagggcac ggacttccag tcccgcggtg gttttatgca ttcccagtac ggaaaaagga
    18121 tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc tatcgatacg atcggacatg
    18181 cttgtcttcc tgttttattg tgtcacccca tccaattgga tttttgactt ctggttgtga
    18241 catttttttg acctgaacgg cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc
    18301 aggtaaggct gtgaatactc atgtattcag ccacccttaa agaatagcca atgctctatt
    18361 taactcccgg taaatcatga aacatctgcg cttactcctg tattacgcac taacaggggc
    18421 ggcatcgcgc cccagattta atgaataaag attacgccag ttgacgaagc atccgacgca
    18481 gcggctccgc ggccccccac agcagctggt cgcccacggt aaaggctgac aggaactctg
    18541 gtcccatatt cagcttacgc aggcggccta ccggcgtggt cagcgtgccg gtaacggcag
    18601 ctggggttag ctcacgcata gtgatttccc gatcgttcgg aacgactttc gcccacggat
    18661 tgtgcgcagc cagcagttct tccacggtcg gaatcgacac atcttttttc aatttaatag
    18721 tgaatgcctg gctgtggcag cgcaatgccc cgacacgcac acataaacca tctaccggaa
    18781 ttacggaaga tgtgttgagg atcttgttgg tttccgcctg ccctttccac tcttcgcggc
    18841 tctgaccgtt atcgagctgt ttgtcgatcc acggaatcag gctacccgcc agcggcacgc
    18901 caaagttatc caccggcagc tcaccgctac gggttaaggt tgtgactttg cgttcgatat
    18961 cgagaatagc agaggacggg gtcgcgagtt catctgctac atggccatac agatggccca
    19021 tctgggttaa taactcacgc atatgccgcg caccaccgcc ggaagcggcc tggtaggttg
    19081 caacggacac ccaatcaaca agatcattgg cgaataaacc acccaacgac atcaacatca
    19141 ggcttacggt acagttaccg ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa
    19201 tgacgtcctg attgacgggg tcaagaatga tgatggcgtc atctttcatg cgcagagacg
    19261 atgctgcgtc aatccagtaa ccctgccatc cgctttcacg aagctttgga tagatttcgt
    19321 tggtataatc gccgccctga caggtcacaa tgatatcgag ggcctttagc gcctccagat
    19381 caaaggcatc ctgaagtgtg ccagtggttc cgccaaaaga cggcgcagcc tgaccaagct
    19441 gagaagtaga aaagaagaca gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt
    19501 gcatgagaac ggagccgacc ataccgcgcc agccgataaa accaacattt ttcataagcg
    19561 ttttttcctg caaagatgtg tgctgtataa atgtgccggt ctcctcttgg cacatctttc
    19621 accatacaaa aagcagccaa agtcgcaagt gaaattaatc aatgatagcg aagccatcag
    19681 taatgcgact tatcctgctt tgttagcacg cagaaagtcc gcggcaatta tcagggaatt
    19741 tgagttatga atgaaatcat ttctgcagca gttttattga tcctgattat ggatccgctc
    19801 ggaaacctac ctattttcat gtccgtactg aaacatactg aaccgaaaag acggcgggca
    19861 atcatggtgc gagagttgct tattgctctc ctggtgatgc tggtgttcct gtttgcgggt
    19921 gagaaaattc tggcatttct cagcctgcga gcagaaaccg tctccatttc tggcggcatc
    19981 attctgtttc taattgccat taagatgatt ttccccagcg cttcaggaaa tagcagcggg
    20041 cttccggcag gtgaagagcc atttatcgtg ccgctggcaa ttccgctggt cgccgggcca
    20101 actattctcg ccacgctgat gttgttgtct catcagtacc cgaatcagat gggacatctg
    20161 gtgattgctc tgctgctggc ctggggcggc acctttgtca tcctgctaca gtcttcgcta
    20221 tttttacgtc tgctgggcga gaaaggggtg aacgcacttg aacgcctgat gggattgatt
    20281 ctggtgatga tggcaaccca gatgttcctc gacggcattc gaatgtggat gaaggggtaa
    20341 ggttcaaaca ggtgctgcac acaaccagac gcggcacctg ttttattacc tgaactgata
    20401 atgaccttcg cccaatcgga taacccttcc ctcttcttct gcgcggttca gccacacact
    20461 aatttgttcg atggttaaac cagtacaaga cgccagcctt tcaatactta tcgattcctt
    20521 tgcaaggatc gccagttccg ccacaaagag ttgataaaaa tcgacaggcg tatacgcggg
    20581 cattactgcg taatttgcgt catcttcatg cagcgaaaac agttctggtt gagcgttacg
    20641 gagtgctctg tcatacatca gcccaggaac gtctggcagt ctgatcgtca cattctcagc
    20701 ctgttcagca ctccatgacg ccccattttc tactaatcgt gaattggctg attgcatatc
    20761 ctgatttggt ttaacctgca caggcagcca ctgatgtttt aatgcctcca tcgccccggt
    20821 tatcgttcca ccggtcattc ccgcacgtac aaccattgcg ctttcagcaa ggcaataaat
    20881 atatttattt cgcgccatcg cattgccgac ggtgaaacgt acctctgggt aaaacggcga
    20941 aatcaacacc aggttgcctg ctataagccc ttcacgccat ttcactaacg tactcgtttt
    21001 taacaagcta tcagctaata cgccaacggc agttcccccg gcctccagtg ccgacgccat
    21061 tgcacattca tcaatacctc gcgcaccacc agagataacg caaatcccct gttgggccag
    21121 tttcgcggcc agttgttggg tatagcgcaa atcgtcagtc ggagcatctc gcgagccaac
    21181 aatcgccata ccttccgctt gcagtaatgc tttattcccg cagccaaata aaacgggagg
    21241 tgcatccgtt cgcaatcggt ttttcaagcg aacgggataa tcagcatctc cgcgggttaa
    21301 gatccataaa ccggcgcgtt gccacttatc taccgccaac gccagactat gaccacgcgc
    21361 cagcaagcca agaatacgct cctgagaaat acgcggatcg ctccagcctg taagtttctc
    21421 ttgcggttgc ggcaccagaa gctcggcggg actgatacgt tggtgcttca gccataatgc
    21481 aaacttcccc cattcactat tactaagagg tttatactta ctcgccgccg cgcgagaaaa
    21541 atcgctggtt agcaggagag ttgcttgtgc attggctgaa agattcatca attttttacc
    21601 gaggtagaag caagggcaag aggataaacc gtcgggcaac ctgcctggcg taacagtgct
    21661 gttagaactg tcagcgtcca tgcagaatcc acgatatcgt caaccagcaa cgccggacca
    21721 ggcatcaaag gaggggtaat cacaaacgcc ccgtcgagat tttgacactg gtggaaacgg
    21781 ttttgctgca ttttctgcgg tggattgtcc acgacttttt caatggcatc aataaaaggt
    21841 aagccaagtt tcgccgccag tcgccgggca aaatcaggaa ccaggtcgag gtgctttaat
    21901 gaaggaacgc agcataccca ggttggttca ggatgcggat tccagcgttg gcgaaccatt
    21961 tccgcacatg cctccaccag ttcatcactg aatcgccccg tttttttctc ctgtgctacc
    22021 tgctttcccc acccggaata tccccaacga gaaaggattc gccccgtaga tccttgtaaa
    22081 ctcgcaggca agttcccttt aaatccgtat tgcgtaaaag ccccagctgc cacctgttta
    22141 ttgagattta atgacaggtc agcgtgttta ataaataagt ttgccgcatg gagtaagccg
    22201 ctatcaatgt cgggcgacaa caacggatgc tgaagacaac tgctacattt accacaatgt
    22261 tcggcacctg gctcatcgag tgcgcggcgc agaaattgca ttttgcactc cttggtcgtg
    22321 atatagcttt caacgtctgc cagctcactt tttctctgat tcatcaaatg gttaattcgt
    22381 tcatggtcag gagaaaaact gacaattgtt ctgcgccata atttctcggt atacaccaca
    22441 ggcgatggat tttcagctac caataatttc agtgcttttt ctatttgccc ataacggaga
    22501 ttcgtccgtt gttcaatgcc tcgtaaggta agaccgtcat tcacgctaag tacgttgagt
    22561 atttcatgaa tttgcgcctc cgcaggaaag gcgctttcac ggaagaattt atgaatagca
    22621 cggtcttcac tgccgcaaag cagtatgcca acagcagaat cgataccgcg accggcacgc
    22681 cccacctgtt agtagtaacc aactatcgaa cctggcatct ggtaatgaat aacaaacccc
    22741 aaatccggtt tgtcaaagcc cattcccagc gccgttgtcg caaccagcac tttaatttta
    22801 tttgccagca acgcctgctc cagatattcc ctggcagtat tagaatcttc cgcgccttca
    22861 caagtcacgc cgctgtagta agcaaatgca ctgataccat ttttccttaa ccactcggcg
    22921 acaagttcgg catcgcgagt agtcagggta taaacaattc ccgatttgga aaactgaggg
    22981 ataaccgttg ctaaccatgc cagacgggat gagtgttcac ctaagactaa ggcatcaagc
    23041 gccaaacttt cgcgagccag cgttccacgc tgaatcacaa tgtcacccaa ttgctgacgg
    23101 atatcctcaa cgacacggtt attcgctgtc gcggttgtac ccagaacagg ggtattcgca
    23161 ggtagttgcg caaaatatct aatatacgtc ggtaatccgg gcgaaaatcg tggccccaat
    23221 cagagatgca gtgcgcctcg tcgaccacaa gcaagccaat atgatcggct ataggatata
    23281 aaaccgtttc gataaagtcc tggtttgcca aacgttccgg ggaaaccagc aagcaatcga
    23341 cctcaccttg cagcaactta tcgctaatgc gctgccattc ttctctgttt gtagaattca
    23401 acgtttcagc cgtaatacct aaccgttcag cggcggcaac ctggttacgc atcagggcaa
    23461 gcaaaggaga gatgataatt gtcgggccag cgccgcgatc gcggaagatt ttactggcga
    23521 tgaagtacac agcacttttc ccccagccag tgcgttggac aaccagcagt tttcgccgtt
    23581 ggttcaccac cgcgtctatg gcttcccatt gcccttccct gaaagtagcg gtggtatttc
    23641 ctaacatccg ctgtaaaagc aattcagctg cgtgcttttc catctttatc tcctgcgatc
    23701 tttaggcagt gattagaacg gattatttgc aggtaaattt gcgagagaga tcaaaaagat
    23761 gaagagagga gaaaagagaa atgaattact gcgagcaagc tcgcagtaat ttgtcgcagt
    23821 aattaactca acagctggaa cgcaatcatc ccaacgatgg caccgacagt gccgaggatg
    23881 gtttccatca tggtccaggt tttcagcgtt tcggcttcgg tcgcgccggt aaatttaccg
    23941 aacaaccaga aaccagcgtc gttaacgtgg ctgacaacaa tcgaaccacc ggcgatacaa
    24001 atcgacagcg ccgccatttg cgcaccggag tagttcagtt gttcaataac cggcatcacc
    24061 agtcccaccg ccgttaaaca ggcaacggtt gcagaaccct gaatgatgcg cactgcagcg
    24121 gccagcacga agcaggtgat ggcaatcggc aggcccatgc cggttaacgc ttcgcccagt
    24181 gccggaccta cgccagagtc aaccagcacc tgtttgaaca cgccgcccgc accaatcacc
    24241 agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa tctccatcac tttgtctttt
    24301 ggcatgccct gacgcattgc caggccataa atcgctacca gacaagcaac cagaatcgcg
    24361 gtaaacagat gaccaataaa ctcgaaccat tcgtaagcgg tagagccttc cggcacaaaa
    24421 cgcgcggcaa tggttttcag ccctaccagc accagcggca gcaggatcag cgacaggctg
    24481 aatccgaagg atggcatttt gccttcgccg agatgcggtt cgctgatgtc gtcaggaata
    24541 tgcagctcaa cgtaacggct gatgaagtta ccccacagcg gcccggcaat aatcattccc
    24601 ggaattgccg cacacaggcc aatcaggatc atccagccaa aatcggcgtt catctgcgat
    24661 gccagcagca ttggcgctgg ccctggcacc aggaacgccg ccgcggccgc cacgcctgcg
    24721 aataatggga ttaccagctt caccaggttc gtaccggtgt ggcgcgccat tgagaaagca
    24781 acgctaatca gcagaacaat cgccacttca aagaacagcg gtagcgcaca gaccagcccc
    24841 gcaaggccga tggcataatg cgcgcggctg tgaccgaagg atttaagcat tttgacggca
    24901 atctgatcga ctgcgccggt ttcatgtaag atcttgccaa acatagctcc cagggcgaca
    24961 accaccgcca ggaagccgag ggtgcctccc atcccttttt ccatcgtcgc tgcgatttta
    25021 tcgagcggca taccggaaaa aaggccagcc cccatggaca ccaccattaa agccaggaaa
    25081 gcgtgcatac gcgccttcat gactaaaaac agcagcagta aaacagaccc tactgctgtt
    25141 aaaacaagcg ttaatgtagt cactacttat ttgccttttt taataacctc aatggtgctt
    25201 gccacaacac cttccagcgg ttgatcgata tccaccacca gtacatcggt ttcgtccgca
    25261 cccggctcct gcagcgtttc aaactgcgtc accaacattt gggttttaaa gaaatggcct
    25321 ttgcgcgctt tcaggcggct ttcaatcaca tcaaaatcgc ctttcaggta gatgaaagag
    25381 agattcggat taccttcacg cagcaagtca cgatagtgtt ttttcaatgc agaacagacg
    25441 atcagcgaca ctttattagt gcgctgcata gcaaacgcgg cgtcgttcag cgcctgcaac
    25501 cacggtttgc gatcgtcgtc attcagcggt tcgccagacg ccattttttc gatattgcgc
    25561 cgtggatgga ggaaatcgcc atcaagaaac gcggcatgaa gttgatgcgc cacttcactt
    25621 gcgaccgcag atttgccgct gcccgatacg cccatcaaga cgtaaatgtg gtgatcatgg
    25681 ttagtcgtgc tcaaagtggt gcccccacaa tacaagaatt aaaaatgtta cgggtaactg
    25741 ttatcggtaa cattgtccag ccggacaata tcagaagcaa tatccatccg tgccttaagt
    25801 gtataagtgt gagctacttc aaatttgtgg gcttaaatag atccgcccgg tgacaaggtg
    25861 aaacctaaat ctaacatttt cggtgtcaca gattcgccac gaatacgcgc cagcaggcgt
    25921 tcagcgccaa tactgcccat ccgctcacgc ggcgtcagca cgctcgcaag tcgtggctcc
    25981 atcacctgac caatgtcatg accgtggaaa ccggcaatcg ccatatcgtc aggaactttt
    26041 aaccccagac gctgacattc aaacgccgcg ccgaccgcca gatcatcatt agtacagaac
    26101 acgccatcca gctgcggata ttcccgccgt gcctggcgaa tcagttcaat accggaagag
    26161 taagaagaag attgctcaac catcacgcta tatggcacca ggcctgcatc cagcatcgcc
    26221 tgttcgtatc ccttctgttt gatgatagta cgttcgtcga gacgtgcgcc gagataggca
    26281 atgtggcgat gcccgcgagc aataatggct gtggtcatct ggcgtgccgc ttcaaagtta
    26341 tcaaaaccga cggcgatatc gagacacggc gactggctgt ccatcaactc caccaccggg
    26401 atccccgcca cttcaatcat ctttaaggtg cgcggcgtgt gggtacgttc ggtgaggatc
    26461 agtccgtcga tattccatga gagcattgat tcgagacgtt cttgctccat ttccggttta
    26521 taaccgtagt gggccagcat ggtctgataa ccgtgcgcgt cggtgacgct ttcgattccg
    26581 cgtaatactt ccgcgaaaac ctggttggtg agagaaggta acaggacgcc aatcgcccgg
    26641 ctggtggcgt tagagaggat atcgggcgca cgattgggaa tatagcccag ttcatcaaga
    26701 gccgcggcaa tcttgccgcg tagagcgacg gaaacctgct ccgggttgcg taaaaaacgg
    26761 ctgaccgtca ttttggtcac gcctacacgg tcagccacat cctgaagtac gggtcttttc
    26821 tttttcatcg tcctgaaggt acaaaagaga tagattctct tagtttaaca cggacgcatc
    26881 agaaccttcc cggggaaaac agtgcttgtt tacaattatt agatatacac taaatcaatt
    26941 gcatcgcatt gtgctaacga cgctgcaaaa ccccggaagc gtacatcagc acgcgaccgg
    27001 agcgagcgag gaagcctgca aaaaggcagg cttcaaagat gccgttgagc tttaaaccgg
    27061 cggcagatcg aacagtaaca cttcgctgtc gctatccgca tggatggaga ttgcctgctc
    27121 atcccagatt gccagaccat cgctgatcga ggctttcacg ccgttaatgg tgacattgcc
    27181 tttcaccacc tggatccaga cgcggcgttc agcggcaatc tgatgcaccg actgctcatc
    27241 tttcagcaac gcccagcggt acagttccat atcctgatgc actttcaatg aaccatcacg
    27301 cgcatctggc gaaagcacca gttgtttgcc ctgcactgca tcgaagcgac gctgttcata
    27361 acgcggcgta atgccgtttt cttccggcat gatccagatc tgatacaggt gcagacgctc
    27421 ggtgctgctt gggttgtact ctgagtgacg aatacccgta cccgcactca taatctggaa
    27481 ctcgcccgcc ggaacctgct ctttattgcc catgctgtcc tggtgctcaa cagtaccttc
    27541 cagcacgtag gtcaaaattt ccatatcttt atgcgggtga gtgccgaagc cctgccctgc
    27601 ttcaatcacg tcgtcgttaa tcacgcgcag cgcggagaag cccataaagt tcggatcgta
    27661 atagttggca aaagagaaag tatgccagga gtccagccag ccatgatttg catgaccacg
    27721 ttcatttgct ttgcgtaagt agatcatttg tattcgcccc ctgaatgatt tcgatgggct
    27781 tagtgtggac ccgatctgcc tgggatgata gagggtgaaa attgacccct ctgttcaaaa
    27841 aaattgaact attcgagggg ccatcaaact tacttcgcga gagtcactgt ggagggagag
    27901 gcttgctcga atccacgttc aagtatttcc agattcgtaa gaacttcaga ttccttgacg
    27961 taatttggcg caccgtgggt gatggtttga tacaacgcat cataaacgcg cccgtaatcg
    28021 cccatctccg gcttcatctc ttctctgacc gtcacgccct cgtcattgac atactccagc
    28081 acaccgaccg aatcatccgc tgcgaatccc ggttcgcccg gcataatatt agccttcagg
    28141 ctggtttcct gctggtcgat accatattta ataaacgaac ctttcttacc gtgaacgata
    28201 aatttcggat aatcgatttt caccagatgg ctggttttga cgatggcttt tagatcgcca
    28261 taaaacagtt gcgcttcgaa agtatcgtca ggattggctt tattgcgcag gctgcggatg
    28321 tcataagcga cgtgatccgg gcgaccgaac agagaaataa tctggtccat cgtatgcaca
    28381 ccaaggccat agaacgcgcc atcctgcggc agcccaggtt tggtttctgc caccgggcgg
    28441 taatagtcaa aatggctttc cacttcgaca atctctccca gcttgccgct ttcaatcgct
    28501 tttttcgccg tcaggaagca ggaatcaaag cgacgattct gatatggcgt gacggtcagc
    28561 cctttgcttt tcgccagtgc aaacagctct ttcgcctgcg caagtgtcgg agtgaacggt
    28621 ttttcgacca gcacattttt cccggcttcc atcgcgcgct tcgcgtactc gaagtggctg
    28681 tccgcatggg tgcagacaac aaccagctta acatcgggat cgtttagcac ttcgttgaga
    28741 tcgctggtga aatggatatg agagtaaatg ggagcttgtt cttccggctt tgcatggcgg
    28801 cgaaaaatat gcgcgacatg ccagctatcc ttgcggttaa gtacatacgg cagatggtaa
    28861 cgggtggtgc ttttgccgaa gccaataaag gcgcagttga tgaccatgat ttcgtccttt
    28921 ttaaggtggt tatttacacc ttagcgcaaa gcggacgtgg ttcctactgg agtgcgcata
    28981 acgaacacaa gcacttcccg aggataaatt gagaacgaaa gatcaaaaaa aaagccagca
    29041 cccggctggc taagtaatac tggaagcaat gtgagcaatg tcgtgctttc aggttctccg
    29101 cgagggtctt cctgatcgcg agacaataat aatcattctc attcgcactt gtccaacact
    29161 ttttgcaaaa aaatgcattt gactcgcatt cgaaagtcaa tgatgttgaa agggacattt
    29221 accccaaaga ggacaaagga atgagtgaga tagtgatacg ccatgctgaa acacgggatt
    29281 acgaggccat caggcagatt cacgcccagc cggaggtgta ttacaacaca ctacaggtgc
    29341 ctcatccttc cgatcatatg tggcaggagc gactcgccga tcgtcccggc atcaagcaac
    29401 tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac cattgacgtg caacagcgcc
    29461 cacgccgcag tcatgttgcc gattttggta tctgtgtcga cgcccgctgg aagaatcgcg
    29521 gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg cgacaactgg ttgcgggtag
    29581 atcgtattga gctaacggtg ttcgtcgata acgcgccagc aattaaggtc tataaaaaat
    29641 tcggctttga gattgaaggg actggcaaga agtacgcatt acgtaatggt gaatatgttg
    29701 atgcgtattt tatggcgcgg gtgaagtaaa gaatattctc aaattgcctg ttaccaatgt
    29761 gaataattag ttttgttcat ctaatttgag tttttcctta tattaccacc atcattttga
    29821 aacaatttgt aacataaaga gacctttgaa tcattactcg ttaatatttc ttattaaaga
    29881 gaacatattt tcgcaagcaa tagttattac tgtttgttta tggatagaga gtctacttaa
    29941 tgggtacagc agctataagt catttacgat atgatcttaa caaatatgcc ctttctttac
    30001 gcaaaaccgc aacacttgct tcaacatttt t
//
LOCUS       DAZKFZ010000040        29052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000040 DAZKFZ010000000
VERSION     DAZKFZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(73..1548)
                     /gene="ampG"
                     /locus_tag="ACHOQW_001621"
     CDS             complement(73..1548)
                     /gene="ampG"
                     /locus_tag="ACHOQW_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP9572713.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1592..2170)
                     /gene="yajG"
                     /locus_tag="ACHOQW_001622"
     CDS             complement(1592..2170)
                     /gene="yajG"
                     /locus_tag="ACHOQW_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9572714.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2475..2792
                     /gene="bolA"
                     /locus_tag="ACHOQW_001623"
     CDS             2475..2792
                     /gene="bolA"
                     /locus_tag="ACHOQW_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP9572715.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3136..4434
                     /gene="tig"
                     /locus_tag="ACHOQW_001624"
     CDS             3136..4434
                     /gene="tig"
                     /locus_tag="ACHOQW_001624"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9572716.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4679..5302
                     /gene="clpP"
                     /locus_tag="ACHOQW_001625"
     CDS             4679..5302
                     /gene="clpP"
                     /locus_tag="ACHOQW_001625"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9572717.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5428..6702
                     /gene="clpX"
                     /locus_tag="ACHOQW_001626"
     CDS             5428..6702
                     /gene="clpX"
                     /locus_tag="ACHOQW_001626"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP9572718.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6890..9244
                     /gene="lon"
                     /locus_tag="ACHOQW_001627"
     CDS             6890..9244
                     /gene="lon"
                     /locus_tag="ACHOQW_001627"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP9572719.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9435..9725
                     /gene="hupB"
                     /locus_tag="ACHOQW_001628"
     CDS             9435..9725
                     /gene="hupB"
                     /locus_tag="ACHOQW_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP9572720.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9917..11788
                     /gene="ppiD"
                     /locus_tag="ACHOQW_001629"
     CDS             9917..11788
                     /gene="ppiD"
                     /locus_tag="ACHOQW_001629"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9572721.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            11939..12310
                     /gene="ybaV"
                     /locus_tag="ACHOQW_001630"
     CDS             11939..12310
                     /gene="ybaV"
                     /locus_tag="ACHOQW_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9572722.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12416..12814
                     /gene="fadM"
                     /locus_tag="ACHOQW_001631"
     CDS             12416..12814
                     /gene="fadM"
                     /locus_tag="ACHOQW_001631"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP9572723.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12866..13561)
                     /gene="queC"
                     /locus_tag="ACHOQW_001632"
     CDS             complement(12866..13561)
                     /gene="queC"
                     /locus_tag="ACHOQW_001632"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP9572724.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13626..15326)
                     /gene="ybaE"
                     /locus_tag="ACHOQW_001633"
     CDS             complement(13626..15326)
                     /gene="ybaE"
                     /locus_tag="ACHOQW_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP9572725.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15426..16244
                     /gene="cof"
                     /locus_tag="ACHOQW_001634"
     CDS             15426..16244
                     /gene="cof"
                     /locus_tag="ACHOQW_001634"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP9572726.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16396..16854
                     /gene="decR"
                     /locus_tag="ACHOQW_001635"
     CDS             16396..16854
                     /gene="decR"
                     /locus_tag="ACHOQW_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP9572727.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16884..18656
                     /gene="mdlA"
                     /locus_tag="ACHOQW_001636"
     CDS             16884..18656
                     /gene="mdlA"
                     /locus_tag="ACHOQW_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9572728.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            18649..20430
                     /gene="mdlB"
                     /locus_tag="ACHOQW_001637"
     CDS             18649..20430
                     /gene="mdlB"
                     /locus_tag="ACHOQW_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9572729.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20611..20949
                     /gene="glnK"
                     /locus_tag="ACHOQW_001638"
     CDS             20611..20949
                     /gene="glnK"
                     /locus_tag="ACHOQW_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9572730.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            20979..22265
                     /gene="amtB"
                     /locus_tag="ACHOQW_001639"
     CDS             20979..22265
                     /gene="amtB"
                     /locus_tag="ACHOQW_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP9572731.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22314..23174)
                     /gene="tesB"
                     /locus_tag="ACHOQW_001640"
     CDS             complement(22314..23174)
                     /gene="tesB"
                     /locus_tag="ACHOQW_001640"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP9572732.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23392..23964
                     /gene="ybaY"
                     /locus_tag="ACHOQW_001641"
     CDS             23392..23964
                     /gene="ybaY"
                     /locus_tag="ACHOQW_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP9572733.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(23997..24308)
                     /locus_tag="ACHOQW_001642"
     CDS             complement(23997..24308)
                     /locus_tag="ACHOQW_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP9572734.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24471..24567
                     /gene="ffs"
                     /locus_tag="ACHOQW_001643"
     ncRNA           24471..24567
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOQW_001643"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24687..25040
                     /gene="ybaA"
                     /locus_tag="ACHOQW_001644"
     CDS             24687..25040
                     /gene="ybaA"
                     /locus_tag="ACHOQW_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP9572735.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25082..26632)
                     /gene="pdeB"
                     /locus_tag="ACHOQW_001645"
     CDS             complement(25082..26632)
                     /gene="pdeB"
                     /locus_tag="ACHOQW_001645"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP9572736.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26796..27266)
                     /gene="ylaC"
                     /locus_tag="ACHOQW_001646"
     CDS             complement(26796..27266)
                     /gene="ylaC"
                     /locus_tag="ACHOQW_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP9572737.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27382..27933)
                     /gene="maa"
                     /locus_tag="ACHOQW_001647"
     CDS             complement(27382..27933)
                     /gene="maa"
                     /locus_tag="ACHOQW_001647"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP9572738.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28105..28323)
                     /gene="hha"
                     /locus_tag="ACHOQW_001648"
     CDS             complement(28105..28323)
                     /gene="hha"
                     /locus_tag="ACHOQW_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP9572739.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28349..28723)
                     /gene="tomB"
                     /locus_tag="ACHOQW_001649"
     CDS             complement(28349..28723)
                     /gene="tomB"
                     /locus_tag="ACHOQW_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP9572740.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7300 a         6968 c         7835 g         6949 t
ORIGIN      
        1 aagcgatgct gatgtttatt tagccgtaat aattacggcg agtgattact acagctaaat
       61 aatatttaca gattacgtca gatgcgtttt tcgtagcgcc agataatcca gcaaaccacc
      121 aagcacgacg ccagaaaggg cgactaaaac cccgacttcc agcagagcag gcaggaaaga
      181 gaagtgcgtc aaatccagcg cgtccatcgt caatagcagt aaccacacgg ccaacaggct
      241 gacgcccgcc gccagtgtcc acatggcaaa ggcataacct gccggatatg cggtacggga
      301 gataaagttg tcatttactc gtgtatattc aagcgtctgg cggcaaacca gcagcaaaat
      361 aagccctggt acggcagcgg cgacggagaa tagatagaat gtcgaccagc cgtgtgcttc
      421 aacaaaccaa cccgccacgg ggccgacata aactcgccct acagcagaaa gcgctgagag
      481 cagggcaaat tgagtagcgg aaaatgactt attacatagc gtcattaaca gcgcgacaaa
      541 ggctgatgtg cccatcccgc cacagaggtt ttcgaaaaag acggctgcgc ccatgctgta
      601 gagatgctta tcagtaatcg acagcagcca gtaaccagcg ttagacgcac cttgtaaaat
      661 gccgaaaatc agcagtgccc ggaacagtga caggcgctgc atcaaaatcc caccgtacaa
      721 tgcgccaaca atggtcgcta gtaagccaag cgttttgttt accacgccta cttcacccgc
      781 atcaaacccg acgccgcgaa tcaaaaacgt ggttgtcagg ctcatggcga atgcgtcgcc
      841 cagcttatac agcacgataa gaagcaaaat aagccaggca ttattgcgac caaagaaatc
      901 tcgcagaggt gcaacaaccg cttgttccag cgttttgggc acaggaatgg tgtcggttgg
      961 ttctggtgca agcaacgtcg cgataataca ggggatcaac agcgccgcca tcaaccagta
     1021 catgccctgc cagcccagcc atttatctgc cagccacagg gctaggccgc cggaaaccag
     1081 catccctaaa cggtaaccca gaacgctgat tgccgcgccc gcaccacgtt cttctgccgg
     1141 gagcacatcg gttttccacg catcgaagac aatatcctgg gaggcagagc aaaaagcgat
     1201 caccaccgcc agcgccgcca tccagcggag ttgggtgcct ggttcgagaa aacccatcgc
     1261 cgcaatggcg actaataaca ggatttgcgt ggcgagcagc caaccgcgcc gccgcccaaa
     1321 aaatgggggc gtgtagcggt ccatcagcgg tgaccagagg aatttaaaaa cgtaagcctg
     1381 gcctaccaga gagaagaaac caatggtttt gagatcgata ttctcgaccg tcatccaggc
     1441 ctgtaaggtg ccggatgtca gggcgagcgg tagcccggaa gcaaaaccca ggatcagcaa
     1501 tatggctgaa cgcggctgtt gaaaaatacg taaatattga ctggacatgg gcgtctacag
     1561 gcccggcttg caccgggcca gagggcagaa attaacgcgc gttctgcttg atgaattcgt
     1621 gaatgctggt gtcctgagac atatcagcga tggtatcggt cagcacactg ttaaccgcat
     1681 cggcgatatt tttgttggag gcctggaacg caccttcaac gttgtagctg gcacgatagt
     1741 ttttggtcat tttgttgcca ttctgcgcgg tagcgatgat ggcgatatcc gctttggtag
     1801 cgatgttgta gcgcacgttg ccctgggaca cgtcagcata cagctggcta acgatgattt
     1861 gcagattaac cgggccattc ggaccaacca tgtaaccacg cgcggtcatc tgtttttcca
     1921 gcacttcttg cagcaggaaa cgcagatcgc gggaggcggt cagggtaacg atttgattat
     1981 cgcgggtgac ttttgccagc gcctgatcgg tacgctgatc ggcaccatta atgcttacgg
     2041 tgacgcccat caggcttgga tcctgctgtg gcagtgtaat cgtcggggaa acttcaatag
     2101 ttgttggcgg tcttgcgcat cctgcaagca taaacagagc aactaacggg aagaggattt
     2161 ttttgaacat gttcgggctc tcagagactc ttaagcgtgt ttggtaaaaa ttcccgccat
     2221 cataacattg ccaacggcga ggggaagtag gtaaggcatg taaattcatc atgttgacga
     2281 aataatcgcc cctggtaaaa gaaacgctga tgcgaggcct gtgtttcaat ctttaaatca
     2341 gtaaacttca tacgcttgac ggaaaaacca ggacgaaacc taaatatttg ttgttaagct
     2401 gcaatggaaa cggtaaaagc ggctagtatt taaagggatg gatgacatct cagcgttgtc
     2461 ggaggagata tttcatgatg atacgtgagc ggatagaaga aaaattaagg gcggcgttcc
     2521 aacccgtatt cctcgaagta gtggatgaaa gctatcgtca caatgtccca gccggctctg
     2581 aaagccattt taaagttgtg ctggtcagcg atcgttttac gggtgaacgt tttctgaatc
     2641 gtcatcgaat gatttacagt actttagcgg aggaactctc tactaccgtt catgcgctgg
     2701 ctctgcatac ttacactatt aaggagtggg aagggttgca ggacaccgtc tttgcctctc
     2761 ctccctgtcg tggagcagga agcatcgcgt aaaaacgcat ttgcaactgt cggcgctttt
     2821 ccagtatgtt gctaaagatt ttatgaaaaa cggcctgcgg gccgttttgt tttgtctgga
     2881 ttttgcgctt tttgcccagc attcagacga aaattgcccg ggaattgtga aaaaatacgc
     2941 gacagcgcgc aataaccgtt ctcgactcat aaaagtgatg ccgctataat gccgcgtcct
     3001 atttgaatgc tttcgggatg attctggtaa cagggaatgt gattgattat aagaacatcc
     3061 cggttccgcg aagccaacaa cctgtgcttg cggggtaaga gttgaccgag cactgtgatt
     3121 ttttgaggta acaagatgca agtttcagtt gaaaccactc aaggccttgg ccgccgtgta
     3181 acgattacta tcgctgctga cagcatcgag accgctgtta aaagcgagct ggtcaacgtt
     3241 gcgaaaaaag tacgtattga cggcttccgc aaaggcaaag tgccaatgaa tatcgttgct
     3301 cagcgttatg gcgcgtctgt acgccaggac gttctgggtg acctgatgag ccgtaacttc
     3361 attgacgcca tcattaaaga aaaaatcaat ccggctggcg caccgactta tgttccgggc
     3421 gaatacaagc tgggtgaaga cttcacctac tctgtagagt ttgaagttta tccggaagtt
     3481 gaactgcagg gtctggaagc gatcgaagtt gaaaaaccga tcgttgaagt gaccgacgct
     3541 gacgttgacg gcatgctgga tactctgcgt aaacagcagg cgacctggaa agaaaaagac
     3601 ggcgctgttg aagcagaaga ccgcgtaacc atcgacttca ccggttctgt agacggcgaa
     3661 gagttcgaag gcggtaaagc gtctgatttc gtactggcga tgggccaggg tcgtatgatc
     3721 ccgggctttg aagacggtat caaaggccac aaagctggcg aagagttcac catcgacgtg
     3781 accttcccgg aagaatacca cgcagaaaac ctgaaaggta aagcagcgaa attcgctatc
     3841 aacctgaaga aagttgaaga gcgtgaactg ccggaactga ctgcagaatt catcaaacgt
     3901 ttcggcgttg aagatggttc cgtagaaggt ctgcgcgctg aagtgcgtaa aaacatggag
     3961 cgcgagctga agagcgccat ccgtaaccgc gttaagtctc aggcgatcga aggtctggta
     4021 aaagctaacg acatcgatgt accggctgcg cttattgaca gcgaaatcga cgttctgcgt
     4081 cgccaggctg cacagcgttt cggtggcaac gaaaaacaag ctctggaact gccgcgcgaa
     4141 ctgttcgaag aacaggctaa acgccgcgta gttgttggcc tgctgctggg cgaagttatc
     4201 cgcaccaacg agctgaaagc tgacgaagag cgcgtgaaag gcctgatcga agagatggct
     4261 tcagcgtacg aagatccgaa agaagttatc gagttctaca gcaaaaacaa agaactgatg
     4321 gacaacatgc gcaatgttgc tctggaagaa caggctgttg aagctgtact ggcgaaagcg
     4381 aaagtgactg aaaaagaaac cactttcaac gagctgatga accagcaggc gtaatttacg
     4441 cagcataacg cgctaaattc gcacaaaggc ccgtcaccgc caggtggtgg gctttttttg
     4501 tcatgaattt tgcatggaac cgtgcgaaaa gcctctttcg gtgttagcgt aacaacaaaa
     4561 gattgttatg cttgaaatat ggtgatgccg tacccataac acagggacta gctgataatc
     4621 cgtccataag gttacaatcg gtacagcagg ttttttcaat tttatccagg agacggaaat
     4681 gtcatacagc ggcgaacgag ataactttgc accccatatg gcgctggtgc cgatggtcat
     4741 tgaacagacc tcacgtggtg agcgctcttt tgatatctat tctcgtctac ttaaggaacg
     4801 cgtcattttt ctgactggcc aggttgaaga ccatatggct aacctgattg tggctcagat
     4861 gctgttcctg gaagcagaaa acccagaaaa agatatctat ctgtacatta actccccagg
     4921 cggggtgatc actgccggga tgtctatcta tgacaccatg cagtttatca agcctgatgt
     4981 cagcaccatc tgtatgggcc aggcggcctc gatgggcgct ttcttgctga ccgcaggggc
     5041 aaaaggtaaa cgtttttgcc tgccgaattc gcgcgtgatg attcaccaac cgttgggcgg
     5101 ctaccagggc caggcgaccg atatcgaaat tcatgcccgt gaaattctga aagttaaagg
     5161 gcgtatgaat gaacttatgg cgcttcatac gggtcaatca ttagaacaga ttgaacgtga
     5221 taccgagcgc gatcgcttcc tttccgcccc tgaagcggtg gaatacggtc tggtcgattc
     5281 gattctgacc catcgtaatt gatgccagag gcgcaactgt gccgctatac ttatccaggg
     5341 cggcacaacg ctgtaagcgg cttgcgcctg agaatggcat ttgcgtcgtc gtgtgcggca
     5401 caaagaacaa agaagaggtt ttgacccatg acagataaac gcaaagatgg ctcaggcaaa
     5461 ttgctgtatt gctctttttg cggcaaaagc cagcatgaag tgcgcaagct gattgccggt
     5521 ccatccgtgt atatctgcga cgaatgtgtt gatttatgta acgacatcat tcgcgaagag
     5581 attaaagaag ttgcaccgca tcgtgaacgc agtgcgctac cgacgccgca tgaaattcgt
     5641 aaccacctgg acgattacgt tatcggccag gaacaggcga aaaaagtgct ggcggtcgcg
     5701 gtatacaacc actacaaacg tctgcgcaac ggcgatacca gcaatggcgt cgagttgggc
     5761 aaaagtaaca ttctgctgat cggtccgacc ggttccggta aaacgctgct ggctgaaacg
     5821 ctggcgcgcc tgctggacgt cccgttcacc atggccgacg caaccacgct gaccgaagcc
     5881 ggttatgtgg gcgaagacgt tgaaaacatc attcagaagc tgttgcaaaa gtgcgattac
     5941 gacgtacaga aagcgcagcg cgggattgtc tacatcgatg aaatcgacaa gatttctcgt
     6001 aagtcagaca acccgtctat tacccgtgac gtttccggtg aaggcgtaca gcaggcactg
     6061 ttgaaactga tcgaaggtac ggtagctgct gttccaccgc aaggtggacg taaacatccg
     6121 cagcaggaat tcttgcaggt tgatacctct aagatcctgt ttatttgtgg cggtgcgttt
     6181 gccggtctgg ataaagtgat ttcccatcgt gtagaaaccg gctccggcat tggttttggc
     6241 gcgacggtaa aagcgaagtc cgacaaagca agcgaaggcg agctgctggc gcaggttgaa
     6301 ccggaagatc tgatcaagtt tggtcttatc cctgagttta ttggtcgtct gccggttgtc
     6361 gcaacgttga atgaactgag cgaagaagct ctgattcaga tcctcaaaga gccgaaaaac
     6421 gccctgacca agcagtatca ggcgctgttt aatctggaag gcgtggatct ggaattccgt
     6481 gacgaggcgc tggatgctat cgctaagaaa gcgatggcgc gtaaaaccgg tgcccgtggc
     6541 ctgcgttcca tcgtagaagc cgcactgctc gataccatgt acgatctgcc gtccatggaa
     6601 gatgtcgaaa aagtggttat cgacgagtcg gtaattgatg gtcaaagcaa accgttgctg
     6661 atttatggca agccggaagc gcaacaggca tctggtgaat aattaaccat tcccatacaa
     6721 ttagttaacc aaaaaggggg gattttatct cccctttaat ttttcctcta ttctcggcgt
     6781 tgaatgtggg ggaaacatcc ccatatactg acgtacatgt taatagatgg cgtgaagcac
     6841 agtcgtgtca tctgattacc tggcggaaat taaactaaga gagagctcta tgaatcctga
     6901 gcgttctgaa cgcattgaaa tccccgtatt gccgctgcgc gatgtggtgg tttatccgca
     6961 catggtcatc cccttatttg tcgggcggga aaaatctatc cgttgtctgg aagcggcgat
     7021 ggaccatgat aaaaaaatta tgctggtcgc gcagaaagaa gcttcaacgg atgagccggg
     7081 tgtaaacgat cttttcaccg tcgggaccgt ggcctctata ttgcagatgc tgaaactgcc
     7141 tgacggcacc gtcaaagtgc tggtcgaggg gttacagcgc gcgcgtattt ctgcgctctc
     7201 tgacaatggc gaacactttt ctgcgaaggc ggagtatctg gagtcgccga ccattgatga
     7261 gcgagaacag gaagtgctgg tgcgtactgc aatcagccag ttcgaaggct acatcaagct
     7321 gaacaaaaaa atcccaccag aagtgctgac gtcgttgaat agcatcgacg atccggcgcg
     7381 tctggcggat accattgctg cacatatgcc gctgaaactg gctgacaaac agtccgttct
     7441 ggagatgtcc gacgttaacg aacgtctgga atatctgatg gcaatgatgg aatcggaaat
     7501 cgatctgctg caggttgaga aacgcattcg caaccgcgtt aaaaagcaga tggagaaatc
     7561 ccagcgtgag tactatctga acgagcaaat gaaagctatt cagaaagaac tcggtgagat
     7621 ggacgacgcg ccggacgaaa acgaagccct gaagcgcaaa atcgacgcgg cgaagatgcc
     7681 gaaagaggca aaagagaaag cggaagcaga gttgcagaag ctgaaaatga tgtctccgat
     7741 gtcggcagaa gcgaccgtag tgcgtggtta tatcgactgg atggtacagg taccgtggaa
     7801 tgcgcgcagc aaggtcaaaa aagacctgcg tcaggcgcag gaaatcctta ataccgacca
     7861 ttatggtctg gagcgcgtga aagatcgcat ccttgagtat cttgcggttc aaagccgtgt
     7921 caacaaaatc aagggaccga tcctttgcct ggtagggccg ccgggggtag gtaaaacctc
     7981 cctgggtcag tccattgcca aagccaccgg gcgtaaatat gtccgtatgg cgctgggcgg
     8041 cgtgcgtgat gaagcggaaa tccgtggtca ccgccgtact tacatcggtt ctatgccggg
     8101 taaattgatc cagaaaatgg cgaaagtggg cgttaaaaac ccgctgttcc tgctcgatga
     8161 gatcgacaaa atgtcttctg acatgcgtgg cgatccggct tccgcactgc ttgaagtgct
     8221 ggatccagag cagaacgtgg ccttcagcga tcactacctg gaagtggatt acgatctcag
     8281 cgacgtgatg tttgtcgcga cgtcgaactc catgaacatt ccggcaccac tgctggatcg
     8341 tatggaagtg attcgcctct ccggttatac cgaagatgaa aaactgaaca tcgccaaacg
     8401 tcacctgctg ccgaagcaga ttgaacgtaa tgcactgaaa aaaggtgagc tgaccgtcga
     8461 cgatagcgcc attatcggca ttattcgtta ctacacccgt gaggcgggcg tgcgtggtct
     8521 ggagcgtgaa atctccaagc tgtgccgtaa agcggttaag cagttactgc tcgataagtc
     8581 attaaaacat atcgaaatta acggcgacaa cctgcatgac taccttggtg ttcagcgttt
     8641 cgactatggt cgcgctgata acgaaaaccg tgtcggtcag gtaaccggtc tggcgtggac
     8701 ggaagtgggc ggtgacttgc tgaccattga aaccgcatgt gttccgggta aaggcaaact
     8761 gacctatacc ggttcgctcg gcgaagtgat gcaggagtct attcaggctg cgttaacggt
     8821 ggttcgcgcg cgtgcggaaa aactggggat caaccctgat ttttacgaaa aacgtgacat
     8881 ccacgtccac gtaccggaag gtgcgacgcc gaaagatggt ccgagtgccg gtattgctat
     8941 gtgcaccgcg ctggtttctt gcctgaccgg taacccggtt cgtgccgatg tggcaatgac
     9001 cggtgagatc actctgcgtg gtcaggtact gccgatcggt ggtttgaaag aaaaactact
     9061 ggcagcgcat cgcggcggga ttaaaacagt gttaattccg ttcgaaaata aacgcgatct
     9121 ggaagagatt cctgacaacg taattgccga tctggacatt catcctgtga agcgcattga
     9181 ggaagttctg actctggcgc tgcaaaatga accgtctggc atgcaggttg tgactgcaaa
     9241 atagtgacct cgcgcaaaat gcactaataa aaacagggct ggcaggctaa ttcgggcttg
     9301 ccagcctttt tttgtctcgc taagttagat ggcggatcgg gcttgccctt attaaggggt
     9361 gttgtaaggg gatggctggc ctgatataac tgctgcgcgt tcgtaccttg aaggattcaa
     9421 gtgcgatata aattataaag aggaagagaa gagtgaataa atctcaattg atcgacaaga
     9481 ttgctgcagg ggctgatatc tctaaagctg cggctggccg tgcgttagat gctattattg
     9541 cttccgtaac tgaatctctg aaagaagggg atgatgtagc actggtaggt tttggtactt
     9601 ttgccgttaa agagcgtgct gcccgtactg gccgcaaccc gcagaccggt aaagagatca
     9661 ccatcgctgc cgctaaagta ccgagcttcc gtgcaggtaa agcactgaaa gacgcggtaa
     9721 actaagcgtt gtccccagtg gggatgtgac gaagttcaag ggcgcatcta ctgatgtgcc
     9781 ttttttattt gtattcggtg actttctgcg tcttgtgggc tgacaattgc ccccgtttct
     9841 tgtcacaata ggcctttgcg cgcatcgata cgttgcgtga ggtacacagt catctacagc
     9901 ggagtgttgt tacaccatga tggacagctt acgcacggct gcaaacagtc tcgtgctcaa
     9961 gattattttc ggtatcatta tcgtgtcgtt catattgacc ggcgtgagtg gttacctgat
    10021 tggcggaggc aataactacg ccgcaaaagt gaatgaccag gaaatcagcc gtgggcaatt
    10081 cgagaatgcc ttcaacagcg agcgtaatcg catgcagcaa cagctgggcg atcaatactc
    10141 cgagctggca gcgaacgaag gctatatgaa aaccctgcgt caacaggtgc tgaatcgtct
    10201 gatcgacgag gcgctgctgg atcagtacgc acgtgagctg aaactgggta tcagcgatga
    10261 gcaggttaaa caggcgattt tcgcgacccc agccttccag gttgatggca aatttgataa
    10321 cagccgctat aacggtatcc tcaaccagat ggggatgacc gccgatcagt acgcccaggc
    10381 gctgcgtaac cagctcacta cccaacagct gattaacggc gttgctggta ccgattttat
    10441 gctgaaaggt gaaaccgacg agctggcggc actggtcgct caacaacgcg tggtgcgtga
    10501 agcgactatc gatgttaacg cgctggcggc gaagcagcct gtgaccgaac aggaaattgc
    10561 cagctactac gaacaaaaca aaaacaattt catgacgccg gaacaattcc gcgtgagtta
    10621 catcaagctg gatgccgcaa cgatgcagca accggttagc gatgcggatc tccagagcta
    10681 ctacgatcag catcaggatc aattcaccca gccgcagcgt acccgttaca gcatcatcca
    10741 gaccaaaact gaagatgaag cgaaagcggt acttgatgag ctgaataaag gcggtgattt
    10801 tgctgcatta gccaaagaaa aatctgccga tattatctct gctcgtaacg gcggcgatat
    10861 gggttggtta gaagatgcca ctatcccgga tgaactgaaa aatgctggtc tgaaagaaaa
    10921 aggccaactg tctggtgtca tcaaatcttc ggtcggtttc ctgattgtac gtctggacga
    10981 cattcagcca gcgaaagtga aatcgttaga cgaagtacgt gacgatgtcg cggcgaaagt
    11041 gaaacacgaa aaagccctcg atgcgtacta cgcgctgcag cagaaagtga gcgatgcggc
    11101 aagcaacgac accgagtctc tggccggtgc agagcaagct gccggcgtta aagccactca
    11161 gacgggttgg ttcagcaaag agaacctgcc ggaagagttg aacttcaagc cggttgctga
    11221 cgctatcttt aacggcggtc tggtaggtga aaacggcgcg ccgggcatca actctgacat
    11281 catcaccgta gacggcgacc gcgcattcgt gctgcgcatc agcgagcaca aaccggaagc
    11341 ggtgaaaccg ttggcagatg ttcaggaaca agttaaggca ctggttcagc acaacaaagc
    11401 tgaacaacag gcgaaagtgg atgctgagaa actgctggtt gatttgaaag ccggcaaagg
    11461 tgcggaagct atgcaggctg ccggtctgaa atttggcgag ccgaaaacct taagccgttc
    11521 cggtcgtgac ccgattagcc aggcggcgtt tgcactgcca ctgccagcga aagacaaacc
    11581 gagctacggt atggcgaccg atatgcaagg taatgtggtt ctgctggcgc tggatgaagt
    11641 gaaacaaggt tcaatgccgg aagatcagaa aaaagcgatg gtgcagggta tcacccagaa
    11701 caacgcacaa atcgtctttg aagctctgat gagtaacctg cgtaaagagg cgaaaatcaa
    11761 aattggcgat gcgttggaac agcaataatc ctgaagccgc ctcgcaaaaa aatgctttgc
    11821 agctgtaaca agaaaaggtc gctttcgcgg ccttttccat ttctgaacat tgccatttgt
    11881 ttactgtttt cactgccgtt aaggtgattc cactgttaac aaacaaggag aaaacagtat
    11941 gaaacacgga attaaagcac tgctcattac cctgtccctg gcctgtgccg gaatgtctca
    12001 tagcgcgctg gcggcagctt ctgtggcgaa accgacggcg gtagaaacca aagcggaagc
    12061 tcctgcagca caaaataaag cagcagtacc ggcgaaagcc agtgacgaag aaggctcccg
    12121 ggtcagcatt aataatgcca gcgcggaaga gctagcccgc gcgatgaatg gcgttggcct
    12181 gaagaaagcg caagcgattg tcagttatcg cgaagagtac ggtccgttta aaacggtgga
    12241 ggatctaaag caggtgccgg ggatgggcaa ttcgctggtg gaacgtaatc tggcggtatt
    12301 aaccctgtaa ttaatttgca tagtggcaat tttttgccag actgaagagg tcataccagt
    12361 tatgacctct gtacttataa cagtttttat aaaaacaacg taaggttatt gcgctatgca
    12421 aacacaaatc aaagttcgtg gatatcatct cgacgtttac cagcacgtaa acaacgcccg
    12481 ctaccttgaa tttctcgaag aagcccgctg ggatgggttg gaaaatagcg acagttttca
    12541 ctggatgacg gcccataaca tcgctttcgt ggtggtgaat atcaatatta actatcgtcg
    12601 tccggcggta ttaagcgact tgttaaccat tacgagccag ttacagcaat taaacggtaa
    12661 aagcggcatc ttaagccagg tcattacact ggagccggaa gggcaggtgg tagcggatgc
    12721 gcttattacg tttgtttgta ttgatcttaa aacgcagaaa gcattagctc tggaagggga
    12781 attgcgcgaa aagctggagc agatggttaa gtaaacgttt tgtggtgccg gatgctcaag
    12841 ccgcatccgg cgacacccgg aataattact tcaacccggt tttctgcttc atcgctgcca
    12901 tcaccgtcgg tttatcggcc agataatgat tcaaaccgtt ggcgcgtaaa ttacatgccg
    12961 cacaatgacc gcaaccgtcg cctttaatgc cgttatagca ggtcaacgtt tcgttacgga
    13021 ctaaatccag tttgccgtaa taatctgcca gcgcccaggt ttccgcttta tcaatccaca
    13081 tcagcggcgt ttcaaaacga atatctttcg ccatgcccaa actgacggca tggtttagta
    13141 ctttcacaaa ctcatcgcgg caatccgggt agccggagaa atccgtttcg cagacgccgg
    13201 taattacggc ttctgctttt acctgatacg catatattgc cgccagcgtc aggaacaaaa
    13261 tattacgccc tgggacaaac gtattcggga tgccatcggc ttcaggttca taatcaggca
    13321 ccggaatgct gtcacgcgtc aggctgctga ccgccagctc gttgagcagc gttacatcaa
    13381 gaaccttatg cgcgcgtgcc cccagtttca gcgccagttc gcgtgccacg tcgatttctg
    13441 cgcgatggcg ctgaccgtaa tcgaacgtca cgcaatggac ttcatcatat tgttgtaatg
    13501 cctgcaccag acaggtggtg gaatcctggc ctccactgaa cacaacgaca gcacgtttca
    13561 tagataatcc tgcttgaaca ataaaagcgt tatggtaacg cctgcgatta acccggacca
    13621 gctattcatt gcgacggcgc gggaagccag gcttcggtaa attcaaacca gccgcgcggt
    13681 gtcagtcgca caccgttcac gccgggaggg gcactaatgc gatagtgata gttgaacagc
    13741 ggcgtcagcg tcgcatctgt cattaactgg ctaaaaaccg ctttcagggc attaaatcgg
    13801 ttttcctcat caggcattac ttgcaccgcg tccagtgtcg attgtagatg tgcgtatgct
    13861 ggagcgtcga aaacatgtgg ccacagcgga tcgcagcgca gccattgctc cagagtatat
    13921 tccggtgctt cgccaattaa tctgtcgccc atcatgaggt ctgcgtgtgc cagtagggtc
    13981 gtgtcgtccc agttttttgc gttatgaaaa ataattgtga gttcacagcc ttccgctgcc
    14041 agtgtcgcct gtaggcgttc tgccatggta tgaagttcta tcgggaggtg ataaaccagc
    14101 gtcaaggttt tcggtagttt gacttcatcc ggtacttgcc aatgcggaat agtccagcct
    14161 ggcagtaatg catgactggc ggtgatcagg ttttctccga cttctaacgt ttgtaataaa
    14221 ccggattgat gaataatgga gatcactttt cgcgcctgcc agagggagag tcggggactt
    14281 ttgcgcaacg ttaaatagca aaaacctaaa ctgatgccac tactgacctg gctgacccgt
    14341 tgcagctcct ccggtttgcc gatggtgatt tgcacgggat gccgacaact ggttcccaaa
    14401 tctttttcga aaagcggcgg agttatccag tactcaaccg ctttaagcag cggatgacgt
    14461 aaatggtaat aatcatggct ttccaggcgc accagttcag ctgtgaattg tgttaagcga
    14521 aaaggacccg tgccaatcag tgggaattgc ggatgcgcca gatggctgca atagctcgcc
    14581 agccggtgcg caagccagta atcggggcga tgtaaaaaga aggtcagaca ctgcggatgg
    14641 gtgacctcaa tacgcttcac gctaataaat aattgatcca gtgctggcag ttgtaacagc
    14701 atcaataatc gctggtgtaa gtgtgaggct tttactgcat cgccgttatg ccagtgtagg
    14761 gttgaacgaa gataaaagtc ccagcgtaac ccgtcagcag aggtttccca gtgatgcgct
    14821 aaatcgccaa tcgggcgctg ggtattatta tcgaagcggg tcaggccgga aaatatctgt
    14881 ccggcgagat gctgctcggc acggccgggc aaaaagcccg gttgtagcgg ttcgagcggg
    14941 cgatagtagg gaatacgcaa cgtgggtgta tcgttttgcc attgtccgcc cataaacggc
    15001 tgtaacagag tgcgcagctc acctggggcc agttgcgcca gctccagcac atcttgctgc
    15061 tttccggttt ccagtgcctt ttccatcatc gcattgcgta gcgattctgg cgtgaccaga
    15121 aagcgtaatt gtccgcgctt tccgcgtcct gactgcgcct gccactccag ccatcccgct
    15181 tcctgtgcct gacgcaacag cgtacgaaca tggcgttcgc tgcaaaaaca gcgttcggcc
    15241 agttcgctga cggtgacggt ttgcggcttt ccggcggaag gttgccacag acgttgatac
    15301 tggttaaggc ggttgagcag tcgcataaaa acccggaaca atattattta actattcact
    15361 attacttccg tatatatcag gtgatactca atcaccatta accgtgtcac agagtggaga
    15421 aagaaatggc tcgtctggca gcatttgata tggatggcac tttattgatg cctgaccatc
    15481 atttaggtga gaaaaccctc tctactttgg cgcgactgcg tgaacgcgac attaccctca
    15541 cttttgccac ggggcgtcat gcgctggaga tgcagcatat tctcggggcg atatcgctgg
    15601 atgcgtattt gattaccggc aacggaacgc gcgtgcattc tctggaaggt gaacttttac
    15661 atcgtgatga tttacctgcg gatgtcgcgg agctggtgct gtatcagcaa tgggataccc
    15721 gagccagcat gcatattttc aatgacgacg gttggtttac cgggaaagag atccctgcgt
    15781 tgttgcaggc atttgtctat agcggttttc gttatcaaat aatcgatgtc aaaaaaatgc
    15841 cactcggcag cgtcaccaag atctgcttct gtggcgatca cgacgatctt acacgtttgc
    15901 agatccagct acacgaagca ttaggcgagc gtgcacattt gtgtttttcc gccacggatt
    15961 gccttgaagt gctgccggtg ggctgcaata aaggcgctgc actgacggtg ctgacccaac
    16021 atttaggttt atcgttgcgc gattgcatgg cctttggtga tgcgatgaac gatcgcgaaa
    16081 tgttaggcag cgtcggtagc ggatttatta tgggcaatgc gatgccgcaa ctgcgcgcgg
    16141 agctcccgca tttaccggtg attggacatt gccgaaatca ggctgtctct cactatttga
    16201 cgcactggct ggactatcca catctacctt attcccccga ataacgagat cccttccagc
    16261 accgggcaat tgcccggttt ttttgcgttg aatttgtcat tttgtgccgt ggtgtttaaa
    16321 ccgcacagaa taaattgtcg cgatttcacc tttaaaatag aattaaaaga gaaaaaaatt
    16381 ctccgtggaa gggctatgtt agataaaatt gaccgtaagc tgctggcctt actacagcag
    16441 gattgcaccc tctctttgca ggcactggct gaagccgtta atctgacaac caccccctgc
    16501 tggaagcgcc tgaaacggct ggaggacgac ggtatcctta tcggcaaagt cgccctgctg
    16561 gatccggaaa aaataggcct cggcctgacc gcctttgtgc tgataaaaac gcaacatcac
    16621 agcagcgaat ggtattgccg ctttgtcacg gtggttaccg aaatgccaga agtgctgggg
    16681 ttctggcgca tggctggtga atacgattat ctgatgcgcg tccaggttgc cgacatgaaa
    16741 cgctacgacg agttttataa gcgtctggta aacagcgtgc cggggctgtc ggacgtcact
    16801 tccagcttcg cgatggaaca gattaaatac accacttctt tacccatcga ataaatatcc
    16861 agaatcaggt caggacacaa cgcgtgcgat tatttgctca attaagctgg tatttccgtc
    16921 gggaatggcg tcgctatctc ggggctgtcg ccttgcttgt cattatcgcg atgctgcaac
    16981 tggttccacc aaaagtggtt ggtattgttg tcgatggcgt gacagaacaa cactttacta
    17041 ccgggcagat cctgatgtgg atcgccacca tggtgctgat tgccgttgtg gtttatctcc
    17101 tgcgttacgt ctggcgcgta ttgctgtttg gtgcgtctta tcaactggct gttgaactgc
    17161 gtgaagatta ttaccgtcag ctaagccggc agcatcctga gttttacctg cgtcatcgca
    17221 cgggtgacct catggctcgt gcgactaatg acgtcgatcg cgtcgtgttt gccgccggag
    17281 aaggggtgct gacgctggtg gattcactgg taatgggctg cgctgtgttg attatgatgt
    17341 ctacgcaaat tagctggcag ttgaccttat ttgccctgtt accgatgcca gtgatggcga
    17401 tcatgattaa gcgcaacggc gatgctttgc atgaacgctt taagctggca caggcggcgt
    17461 tttccagtct taatgaccgc acccaggaaa gcctcaccag tatccgcatg atcaaagcct
    17521 ttggtctgga agatcgccag tcggcgttat ttgccgcgga tgccgaagat accggcaaaa
    17581 aaaacatgcg ggtggcgcgt attgatgctc gtttcgaccc gaccatctat atcgcgattg
    17641 gtatggcgaa cttgctggcg attggcggcg gtagttggat ggtggtgcag ggcagtttaa
    17701 cgctgggcca gctcaccagt tttatgatgt atttaggtct gatgatttgg ccaatgctgg
    17761 cgctggcatg gatgtttaac attgtggaac gtggtagtgc tgcgtacagc cgtattcgcg
    17821 cgatgctggc ggaagcgccg gtggtgaacg atggtagcga acccgtgccg gaagggcgtg
    17881 gcgaactgga tgtaaatatt caccagttca cgtatccgca gactgaccat cctgcgctgg
    17941 aaaacgtcaa tttcgccctg aaacccggtc agatgctggg tatctgcggg ccgactggtt
    18001 ccggcaaaag taccctgttg tcgctcattc agcgtcattt cgacgtcagc gagggggata
    18061 ttcgctttca tgatattcct ctgacgaagt tacaactcga tagctggcgt agccgcctgg
    18121 cggtagttag ccagacgcca ttcctttttt ctgacaccgt ggcgaataac atcgcgctgg
    18181 gttgcccgaa tgccacccag caagagattg agcatgtcgc gcggttagcc agcgtacatg
    18241 acgatatttt gcgtctaccg caaggttacg atacagaggt gggcgagcgc ggtgtgatgc
    18301 tttccggcgg gcaaaaacag cgtatctcca ttgctcgtgc gttattagtc aacgcggaaa
    18361 tcctcatcct tgatgatgcg ctttcggcgg tggacggacg cactgagcac cagatcctgc
    18421 ataacctgcg tcagtggggg cagggaagaa cggtaatcat cagtgcccat cgcctttctg
    18481 cactgacgga agccagtgaa attattgtga tgcagcacgg acatatcgcc cagcgtggca
    18541 atcatgatgt gctggcacaa caaagcggct ggtatcgcga tatgtatcgc tatcaacaac
    18601 tggaggcggc gctcgacgac gctccggaaa atcgcgagga ggccgtcgat gcgtagtttt
    18661 agccaactgt ggccgactct caagcgcctg ttagcgtacg gttcaccgtg gcgtaaaccg
    18721 ctggggattg cggtcctgat gatgtgggtt gcggcggcgg cagaagtcag tgggccgctg
    18781 cttatcagct attttatcga caatatggta gcgaaaaata acctgccgtt gaaagtggtt
    18841 gcagggctgg ctgcggcgta tgttgggctg caactgtttg ccgccgggct acattacgcg
    18901 cagtcgctgc tgtttaatcg ggcggcagta ggcgtagtgc aacagttgcg taccgacgtg
    18961 atggatgctg cgttacgcca gccattaagc gagtttgata cccaacccgt cgggcaggtg
    19021 atttcccgcg tcactaatga cactgaagtg atccgcgatc tctacgttac cgtagtggca
    19081 actgtcctgc gcagtgccgc actggtgggc gcgatgctgg tggcgatgtt cagcctcgac
    19141 tggcgaatgg cgctggtggc gataatgatt ttcccggtgg tgctggtggt aatggtgata
    19201 taccagcgtt acagcacgcc aattgtccgt cgtgtgcgcg cctatctggc ggatatcaac
    19261 gacggcttta acgaaatcat caatggcatg agcgttatcc agcagtttcg tcagcaggcg
    19321 cgatttggcg aacgtatggg tgaggccagt cgttcacact atatggcgag gatgcaaacc
    19381 ctgcgcctcg acggttttct gctgcgtccg ctgctgagtc tgttttcatc gctcattctc
    19441 tgtggcttgt tgatgctgtt tggcttctcc gccagcggca ccattgaagt gggcgtgttg
    19501 tatgcgttta tcagctatct tgggcgactt aacgaaccct taatcgaact gaccacgcag
    19561 caggcgatgc tgcaacaggc tgttgttgct ggtgagcgcg tgtttgagct gatggacgga
    19621 ccgcgccagc aatatggcaa tgatgatcgc ccgttacaga gtggcaccat cgaagtcgat
    19681 aacgtgtcat ttgcttatcg cgatgacaat ctggtgctaa agaacattaa tctctctgtg
    19741 ccttcgcgta actttgtggc gctggtcggg cataccggca gtggtaaaag taccctcgcc
    19801 agtttattga tgggctatta cccgctaacg gaaggtgaga ttcgccttga tggtcgtcca
    19861 ttaagttcgc taagtcacag cgcgctgcgc cagggcgtgg caatggtgca gcaagatccg
    19921 gtggtgctgg cggatacctt cctcgccaac gtgacgctgg ggcgggatat ctccgaagaa
    19981 cgcgtctggc aggcgctgga aaccgtgcaa ctggcggagc tggcacgcag tatgagcgac
    20041 gggatttata cgccgctggg cgagcagggg aataatctct ctgtcgggca aaagcaactg
    20101 ctggcactgg cgcgcgtgct ggtcgagacg ccgcaaatcc tgatccttga tgaggcaacc
    20161 gccagcattg actccggtac tgaacaggcg attcaacatg ctctggcggc ggtgcgtgaa
    20221 cataccacgc tggtagtgat tgctcaccgc ttatcgacta ttgttgatgc cgacactatt
    20281 ctggtgcttc atcgtgggca agccgtggag cagggcactc accagcaact gctggcggcc
    20341 cagggacgct actggcagat gtatcaactg caacttgcgg gcgaagagct tgcagccagc
    20401 gtgcgtgaag aagaatcatt gagcgcctga atagcgcaat atctcatcgt tggtgcaaaa
    20461 atgtaacgca ctgtgcactg tcatagtgcg ttttcatttt caatcttctt aacttcctgc
    20521 tctctttctc gtttttcatt tctggcacac cgcttgcaat accttcttcg tgtagcagaa
    20581 ccattaccga attctgaccg gaggggatct atgaagctgg tgaccgtgat aatcaaacca
    20641 ttcaagctgg aagacgttcg tgaagcgtta tcttccattg gtattcaggg cctgaccgtc
    20701 accgaagtga aaggtttcgg gcgtcagaaa gggcatgccg agctgtaccg gggggcggaa
    20761 tacagcgtca acttcctgcc aaaagtaaaa attgatgtgg cgattgctga tgaccaactc
    20821 gatgaagtga tcgatatcgt cagtaaggcg gcttacaccg gaaaaattgg cgacggcaaa
    20881 atcttcgtcg ctgaattgca acgcgtcatt cgtattcgta ccggcgaagc cgacgaagcg
    20941 gcgctgtaat ctctggcaca cagcaacagg aacgaaaaat gaagatagcg acgataaaaa
    21001 ctgggcttgc ttcactggcg atgctgccgg gactggtaat ggctgcacct gcggtggccg
    21061 ataaagccga caatgcgttt atgatgattt gtactgcact ggtgctgttt atgactattc
    21121 cggggattgc cctgttttac ggtgggttga ttcgcggcaa aaacgtgctg tcgatgctga
    21181 cgcaggtgac ggtgacgttt gcactggtct gtattctctg ggtggtttac ggttactcgc
    21241 tggcgtttgg tgagggcaac aacttcttcg gcaacattaa ctggttgatg ctgaaaaaca
    21301 tcgaactgac ggcggtgatg ggcagcattt atcagtatat ccacgtagcg tttcagggat
    21361 cgtttgcctg cattaccgta ggcttgatag ttggggcgct ggcggaacga atccgcttct
    21421 cagctgtgtt gattttcgtg gtggtatggc tgacgctctc ttacattcct attgcgcata
    21481 tggtgtgggg cggtggtttg ctggcttctc acggtgcgct ggatttcgcg ggtggcacgg
    21541 tggtgcatat taacgccgca atcgccggtc tggtgggcgc gtatctgata ggcaaacgcg
    21601 tgggcttcgg taaagaggcg tttaaaccgc acaacctgcc gatggtcttc accgggactg
    21661 ccattctcta tatcggttgg tttggcttta acgccgggtc agcgggcacg gcgaatgaaa
    21721 tcgcggcact ggcatttgtg aatactgtgg tcgcaacggc ggcggcaatt cttggctgga
    21781 tcttcggtga atgggcgctg cgtggtaagc cttcactgct gggagcgtgt tctggcgcga
    21841 ttgccggtct ggtcggcgtg acgccagcgt gtggctacat tggtgttggc ggcgcgttga
    21901 ttatcggcgt ggtagctggt ctggcgggct tgtggggcgt taccatgctc aaacgcttgc
    21961 tgcgggtgga tgatccctgc gatgtcttcg gtgtgcacgg cgtttgtggc attgtcggct
    22021 gtatcatgac tgggattttt gccgccagct cgctgggcgg cgtgggcttc gctgaaggtg
    22081 tgacgatggg ccatcagttg ctggtacagt tggaaagcat tgccattacg attgtgtggt
    22141 ctggtgtggt ggcatttatc ggctacaaac tggcggatct gacggttggt ctgcgtgtgc
    22201 cggaagaaca agagcgagaa gggctggacg tcaacagtca cggcgagaat gcctataacg
    22261 cataacaagc actgcaaaaa acagccggac ggttttcacc tccggctatt tttttaattg
    22321 tgattacgca tcaccccttc ctgaacggtc gaggcaacca gtacgccgtc ctgggtataa
    22381 aactcaccgc gcacaaagcc acgtgcgctg gacgccgagg tgctctccac gctatacagc
    22441 agccattcat tcaaattaaa cgggcgatgg aaccacatgg aatggtcaat ggtggcaatc
    22501 tgaatccccg gttcgagaaa accgataccg tgcggctgta gagctaccgg caggaagtta
    22561 agatcagaag cgtaaccgag cagatactga tgaacgcgca ggtcatccgg cacgctacca
    22621 tttgcgcgga tccacacctg acgatgtggt tctgcgacgt gacctttcag tgggttatga
    22681 aactccaccg gacggacttc cagcggacga tcgcagatga atttatcttt aagcactggc
    22741 ggcagcaggt gcgccagcga ttgggcgatt tgcgtttccg aaggcaggcc atcaggcgct
    22801 ggcgcggacg gcattgtttt ttgatgttcg aaacccgctt ctggtgcctg gaaagaggcc
    22861 gtcatataaa aaatcggttt gccgttttga atagcagcaa cccggcgggc gctgaagctg
    22921 ttaccgtcac gcagcgtttc gacatcataa ataatcggct tcttactatc gccagggcga
    22981 agaaagtagc tgtgaaacga atgtaccagc cgctcttcag ggacggtctc ttttgcagca
    23041 tacaaggcct gacccacgac ctggccgcca aatacctggc gtaaacctaa atcttcactc
    23101 tggccgcgaa agagtccttc ctcaattttt tccagattta acaatgtcag taaatttttt
    23161 agcgcctgac tcatataact ctccagtaac aaagctgccg cagcaagcca aagtgagttg
    23221 agtataacgc aaatttgcta ctggtccgat gggtgcaatg gtctgaatta cgggctaatt
    23281 acaggcagaa atgcgtgatg tgtgccacaa ttgttgacgt tactattttg ttaaccactc
    23341 ttccggcgag gaaagttagc ccgctggtgc attgataata aggagaaatg aatgaaactc
    23401 gtgcacatgg ccagtggttt agcggttgcg attgcgttgg cggcttgcgc agataaaagc
    23461 gcggatattc agacgccagc cccggctgca aatacgtcta tttcagcaac acaacaacct
    23521 gctatccagc aaccgaatgt ctccggtacc gtctggatcc gtcagaaagt cgcactgccg
    23581 cctgatgctg tgctgaccgt gacactttct gacgcgtcgt tagccgatgc accgtcaaaa
    23641 gtgctggcgc agaaagcggt gcgtactgaa ggtaaacagt caccattcag ctttgttctg
    23701 ccatttaacc cggcagatgt tcagccgaac gcgcgtattc tgttgagtgc ggcgattacc
    23761 gtgaatgaca aactggtatt tattaccgat accgttcagc cggtgatcaa ccagggggga
    23821 accaaagccg acctgacatt ggttccggtt cagcaaaccg ccgtgcctgt ccaggccagt
    23881 ggtggcgcaa caactaccgt accatcgact tcaccgaccc aggtgaatcc atcttcggca
    23941 gttcctgccc ctacgcaata ctaagcgtta ctaacccctt tccatctgga gaggggttag
    24001 tagttccagc gatagcgctg taagtcgatt tgcccgcttc ccgataccat cacaccttct
    24061 gccagtaatg cctgtcgttg acgctgtaaa tccggtccgg ttagcgaaat ggtgccgtgg
    24121 cgattaacca cccggtgcca gggcaaggtg ctgccttcgg ggagacgctt taacacaccg
    24181 ccaacctggc gcgcggcgcg gggcgatccc gccagtttcg ccacatcacc gtaagtggtg
    24241 acataccctt cgggaatagc ggctacgatt tgccagacgc gttggggaaa tgaatcttct
    24301 ttttccatct tttcttcctg agataatttt tcagcataat ctggaaaaac gcccgagtga
    24361 agccgcattg cgcaagaaac cagcatctgg cacgcaatgg gttgcaatta gccggggcag
    24421 cagtgataat gcgcctgcgc gttggttctc aacgctctca atgggggctc tgttggttct
    24481 cccgcaacgc tactctgttt accaggtcag gtccggaagg aagcagccaa ggcagatgac
    24541 gcgtgtgccg ggatgtagct ggcagggccc ccacccattt ctgcctccca ccgtttcgtc
    24601 aaaaaatccc aacatgacta aactttaacc acaactgacg tcgcaagaat tgtctggctg
    24661 cgcagtacgc ttcggaggta tgtctgatga agtatgttga tggttttgtg gttgccgttc
    24721 ctgccgataa aaaggatgcc tatcgggaaa tggccgctaa ggccgcgcca ttgtttaaag
    24781 agtttggcgc gcttcgtatt gtcgaatgct gggccagcga tgtaccggat ggcaaagtga
    24841 ccgattttcg tatggcggtg aaagcggaag agaatgaaga ggttgtcttt agctggattg
    24901 aatacccttc aaaagaggtc cgcgacgcag ctaatcaaaa gatgatgtcg gacccacgga
    24961 tgaaagagtt cggcgagtcc atgccgtttg acggcaagcg aatgatctat ggcggattcg
    25021 agtcaatcat cgacgaatag gtagcgtgac gggctgtgcg gcgtgccgtc agcccaaaaa
    25081 ttcacaaatg ttgctcggcc cagcgtaaga aatcttcttt cggtaatgcc ttgctgtaga
    25141 gccagccctg accgtagtgc acgccatgct ggcgtagcca ctcttcttgt ttactggttt
    25201 cgattccctc cgctaccatt ttcagtttca gtgtttttgc catttcgatg atatgcggcg
    25261 tgacattttt atattccagc gcatcaacga aagatttatc gatcttcaga atgtcgacat
    25321 ccagatcctg taaataactt aaacttgaat accccgtacc aaaatcatca agataaattt
    25381 catggcccgc ctcccggtag cgagaaatta tcggggcgct ggttttcgga tcggcaaact
    25441 cgcgttcagt gagttcaagc gcgatctgtc tgggattaac ctgatagtga ttgatcattt
    25501 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    25561 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaagacg cttcttataa
    25621 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    25681 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    25741 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    25801 catcctgcag ccgatgatgc ggtgactgga tgcggcgcaa aatgcgcagc acaaacatcg
    25861 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    25921 ctttctgcaa cattttcgat gaagcccagg tgataattga aatattcatc tccgggaagg
    25981 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    26041 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    26101 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    26161 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    26221 aatcgttatg tgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    26281 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    26341 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    26401 gggcttcact acagtcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    26461 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    26521 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    26581 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    26641 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    26701 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcgttgt catttgacca
    26761 ggcgcagagc tttacgcctg gtatgtaatc ctaacttaag atgttgattc ggcgcgagcg
    26821 agggtaaaaa tatcgtaaaa agacaattca cctttacgga cgatcatttt ttgcagttgt
    26881 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    26941 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccaa cacgtcgata
    27001 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    27061 aatacaacca gtagccagac agagcctgaa agcgtttcgg actgcaacat caccgccagg
    27121 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    27181 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    27241 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    27301 aacaccctat tttaacgtgg gaatagtaga gggaaagtaa cagatctgcc acaattttgc
    27361 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    27421 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    27481 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    27541 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    27601 tgccgtgtag atatgaacgc ctagtgccaa catacagtta tcaccgatgc gaatagggca
    27661 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    27721 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    27781 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    27841 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    27901 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    27961 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    28021 gatcacatag ggtatgtctt catggcgaaa aagtataaaa ttcttaataa acagccggtt
    28081 atagctccga aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt
    28141 gtcgtacagt ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac
    28201 cgccagttca ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc
    28261 aattgtctgg caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga
    28321 catacttcta cccatggttg taataattct aacaagataa actcgcagga ttctctttcg
    28381 tcgcattgac aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct
    28441 gcatattaat accataacta ctgaacaaca taaaggtgtc atccagatat tcgtcgatct
    28501 gctcaatgag cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa
    28561 tatgctcaat cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc
    28621 catgattgct ttcttcaagg tttgcaaggc agtcatgata cagagtttca cagagaaact
    28681 taagctgtgc gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta
    28741 gcggttgaac taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct
    28801 ctcgtttgtt agcttaacta taagccactc tttgcaggtt ttcatcgcat tattacgaaa
    28861 aattacaatt aggaaggaag cgggacaggg ctcacttttt catactagca ccaacccgca
    28921 gcaggtaaaa gcagtttgct cgaactgttc ataacatttc aggattatcg ccagatgaaa
    28981 ttggtgacac taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa
    29041 gaattaccgc aa
//
LOCUS       DAZKFZ010000041        28262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000041 DAZKFZ010000000
VERSION     DAZKFZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHOQW_001650"
     CDS             complement(<1..209)
                     /locus_tag="ACHOQW_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9572741.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHOQW_001651"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHOQW_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9572742.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHOQW_001652"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHOQW_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9572743.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHOQW_001653"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHOQW_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9572744.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHOQW_001654"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHOQW_001654"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP9572745.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHOQW_001655"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHOQW_001655"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP9572746.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHOQW_001656"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHOQW_001656"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP9572747.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHOQW_001657"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHOQW_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9572748.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHOQW_001658"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHOQW_001658"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9572749.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHOQW_001659"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHOQW_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP9572750.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHOQW_001660"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHOQW_001660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9572751.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHOQW_001661"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHOQW_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP9572752.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHOQW_001662"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHOQW_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP9572753.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHOQW_001663"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHOQW_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP9572754.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHOQW_001664"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHOQW_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9572755.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHOQW_001665"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHOQW_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9572756.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHOQW_001666"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHOQW_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP9572757.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHOQW_001667"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHOQW_001667"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP9572758.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHOQW_001668"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHOQW_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP9572759.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHOQW_001669"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHOQW_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP9572760.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHOQW_001670"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHOQW_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP9572761.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHOQW_001671"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHOQW_001671"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP9572762.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHOQW_001672"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHOQW_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP9572763.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHOQW_001673"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHOQW_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP9572764.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHOQW_001674"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHOQW_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP9572765.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHOQW_001675"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHOQW_001675"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP9572766.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHOQW_001676"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHOQW_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP9572767.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHOQW_001677"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHOQW_001677"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP9572768.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHOQW_001678"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHOQW_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP9572769.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHOQW_001679"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHOQW_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP9572770.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHOQW_001680"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHOQW_001680"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP9572771.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6834 a         7591 c         7134 g         6703 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccggaatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gt
//
LOCUS       DAZKFZ010000042        28194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000042 DAZKFZ010000000
VERSION     DAZKFZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(18..1415)
                     /gene="ccp"
                     /locus_tag="ACHOQW_001681"
     CDS             complement(18..1415)
                     /gene="ccp"
                     /locus_tag="ACHOQW_001681"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP9572772.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1820..3469
                     /locus_tag="ACHOQW_001682"
     CDS             1820..3469
                     /locus_tag="ACHOQW_001682"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9572773.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3520..4122)
                     /locus_tag="ACHOQW_001683"
     CDS             complement(3520..4122)
                     /locus_tag="ACHOQW_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9572774.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4642..5541
                     /locus_tag="ACHOQW_001684"
     CDS             4642..5541
                     /locus_tag="ACHOQW_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9572775.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5590..6603
                     /gene="yhjD"
                     /locus_tag="ACHOQW_001685"
     CDS             5590..6603
                     /gene="yhjD"
                     /locus_tag="ACHOQW_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP9572776.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7015..8337
                     /locus_tag="ACHOQW_001686"
     CDS             7015..8337
                     /locus_tag="ACHOQW_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572777.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8519..10579)
                     /locus_tag="ACHOQW_001687"
     CDS             complement(8519..10579)
                     /locus_tag="ACHOQW_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9572778.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10649..11416)
                     /gene="pdeH"
                     /locus_tag="ACHOQW_001688"
     CDS             complement(10649..11416)
                     /gene="pdeH"
                     /locus_tag="ACHOQW_001688"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP9572779.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11648..12577
                     /gene="kdgK"
                     /locus_tag="ACHOQW_001689"
     CDS             11648..12577
                     /gene="kdgK"
                     /locus_tag="ACHOQW_001689"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP9572780.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12673..14169)
                     /locus_tag="ACHOQW_001690"
     CDS             complement(12673..14169)
                     /locus_tag="ACHOQW_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9572781.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14390..15676)
                     /gene="dctA"
                     /locus_tag="ACHOQW_001691"
     CDS             complement(14390..15676)
                     /gene="dctA"
                     /locus_tag="ACHOQW_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP9572782.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            complement(15859..17847)
                     /gene="hmsP"
                     /locus_tag="ACHOQW_001692"
     CDS             complement(15859..17847)
                     /gene="hmsP"
                     /locus_tag="ACHOQW_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP9572783.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17929..21402)
                     /gene="bcsC"
                     /locus_tag="ACHOQW_001693"
                     /pseudo
     CDS             complement(17929..21402)
                     /gene="bcsC"
                     /locus_tag="ACHOQW_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            complement(21384..22490)
                     /gene="bcsZ"
                     /locus_tag="ACHOQW_001694"
     CDS             complement(21384..22490)
                     /gene="bcsZ"
                     /locus_tag="ACHOQW_001694"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP9572784.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22497..24863)
                     /gene="bcsB"
                     /locus_tag="ACHOQW_001695"
     CDS             complement(22497..24863)
                     /gene="bcsB"
                     /locus_tag="ACHOQW_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP9572785.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24874..27498)
                     /gene="bcsA"
                     /locus_tag="ACHOQW_001696"
     CDS             complement(24874..27498)
                     /gene="bcsA"
                     /locus_tag="ACHOQW_001696"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP9572786.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPRPLDPVRYLIQTCWLLIGASRKETPK
                     PRRRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTF
                     SLILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWW
                     RYTSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPS
                     VDIFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIA
                     RTTHEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPH
                     HFFSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGG
                     IAVETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFR
                     LDNPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIAL
                     FVLPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAK
                     GGLVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLI
                     VLGGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQ
                     ILEGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTW
                     ALWQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPR
                     RPERSETAQPSDQALAQQ"
     gene            complement(27495..28194)
                     /gene="bcsQ"
                     /locus_tag="ACHOQW_001697"
                     /pseudo
     CDS             complement(27495..28194)
                     /gene="bcsQ"
                     /locus_tag="ACHOQW_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
BASE COUNT         6640 a         7822 c         7113 g         6619 t
ORIGIN      
        1 tcgggcgatc aaattaatta ttgtttatcc tgcatatacg gcgtgtaaac cccgttcaga
       61 ctgtgcagga aagctacgat atcatccaca tcctcctgcg gcagctcttt gcctacctga
      121 tagcgcaaca tcagtttcac cgccccgtcc agcgtcggca cgtcaccgcg atggaagtac
      181 ggtgccgtta aagcaacgtt acgtaaaccg ggtactttct gacgcaattt atcacgctct
      241 tctttagtca cattcatacg accaatatcc gccgccgtaa tttccccaaa gttaaagtct
      301 tttttcagcc ccaacggttc gaaggaacgt ccgccgagaa taattccacc atgacaagtt
      361 gcacatttat tatctttaaa taattgatag ccttttttct gttgcgccgt cagcgcattt
      421 tcatccccac gcaaccattt atcaaatggg gaatccggcg taattaatgt tttctcaaat
      481 tcagcaatgg catcagtaat attttcgcca ctgaaacctt gcggatagac gtcgaggaac
      541 tgcgctttaa gctgcggatc tttttccagc ttagcaataa tttcgtccca ggattttgac
      601 gccatttcaa tcgggttcaa cggcggtcca ccagcctgat cctgcaatgt tgccgcacga
      661 ccatcccaga actgctcaac gttaaatact gagttaaata ccgtcggcgc gttaatcggc
      721 ccaactgcgc caccaacacc aatcgatgtt tttctgccat cgacgccccc cgcattcaac
      781 gcatggcaat gagcgcatga aatggtgcta tcagccgata aacggggatc gtgatacagc
      841 gcaaagccca acgccacttt ttgcgcatcg gtaggcagtt tttgcgggat gggctgcacc
      901 ggttcattgc gatgctccgg agcagtatca ttgctggcgt aatattccgc gcgctgtttt
      961 gcaatccagg ccagtatttc cgcccgctct tcatcactca ccttacccgc ccagtgtagc
     1021 gcggtataac gcgttggcgg catagtttca tactgcatca cccattcaat cttattcaga
     1081 tcgctttgcg aaacgggttt atcagccagc agagcagcac gcacggcctc gaggttaaaa
     1141 gatttatatc caagcttaat gtcgtaatcc atcaactgtt tcgcgccagg aatataataa
     1201 taggcgggta attctgccga aggcgtgtgg caatagtcgc atcctttttc gcggagaaag
     1261 cctaaaacct tattattttc actgacagca gatgcctgaa catcggcctg tttactgcgt
     1321 ttattatcgt ggtaccacac ataaccagat aaccctaaat agcaaatcgc gacgccagcc
     1381 aggccgatcg cggtaatacg tgagaccatt ttcattattc ttccctcacg gtggtgagtt
     1441 attgtgaccc agaaaatttt ccagaatgct gcaaacgtct ctgcgagaca aagccatcat
     1501 aaaaaacagg gagatacgct ttttgatagg aatcaatcaa ttacatgaat ataaactatc
     1561 aataagatag cctgtgccta ttacccagat caggaatggc atatcatgat tgcggcaaga
     1621 gcaggattga atgttctgaa aatgaaaaac aacacgaaat ctgtattttt ataaattcca
     1681 ttgatattag tgcgttatat tttttgacag gttaataaac gtgatccacc gcacgctttg
     1741 tcgcccacca ggcggagcga atgactaccc ttaaagaaaa gcccgataat tagcgacgaa
     1801 tttcggaggt tggatcctta tgctcaatca gaaaattcaa aaccctaatc cagacgaact
     1861 gatgatcgag gtcgatctct gctatgaact ggacccgtat gaattaaaac tggatgagat
     1921 gatcgaggca gaaccggaac ccgagatgat tgaagggctg cccgcctctg atgcgctgac
     1981 gcctgccgat cgctatctcg aactgttcga gcatgttcag tcggcgaaaa ttttccccga
     2041 cagtaaaacc tttcccgact gcgcacccaa aatggacccg ctggatattt taatccgcta
     2101 ccgtaaagtg cgccgtcatc gtgattttga cttgcgcaag tttgttgaaa atcacttctg
     2161 gctgccggag gtctactcca gcgagtatgt atcggacccg caaaattccc tgaaagagca
     2221 tatcgaccag ctgtggccgg tgctaacccg cgaaccacag gatcacattc cgtggtcttc
     2281 tctgctggcg ctgccgcagt catatattgt cccgggcggc cgttttagcg aaacctacta
     2341 ttgggattcc tatttcacca tgctggggct ggcggaaagt ggtcgggaag atttgctgaa
     2401 atgcatggcc gataacttcg cctggatgat cgaaaactac ggtcacatcc ccaacggcaa
     2461 ccgcacctat tatttgagcc gctcgcaacc accggttttt gcgctgatgg tggagttgtt
     2521 tgaagaagat ggtgtacgcg gtgcgcgccg ctatctcgac cacctgaaaa tggaatatgc
     2581 cttctggatg gacggtgcag aatcgttgat ccctaatcag gcctatcgcc atgttgtgcg
     2641 gatgccggac ggatcgctgc tcaaccgtta ctgggacgat cgcgacacgc cgcgtgacga
     2701 atcctggctt gaggacgttg aaaccgcgaa acattctggt cgcccgccca acgaggtgta
     2761 ccgcgattta cgcgcggggg cggcctccgg ttgggattac tcttcccgtt ggctccgtga
     2821 tactggtcgt ctggcgagca ttcgtaccac ccagttcatc cccatcgatc tgaatgcctt
     2881 cctgtttaaa ctggagagcg ccatcgccaa catctcggcg ctgaaaggtg agaaagagac
     2941 agaagcactg ttccgccaga aggccagtgc ccgtcgcgat gcggtaaacc gttacctctg
     3001 ggatgatgaa aacggcatct accgcgatta cgactggcga cgcgaacaac tggcgctgtt
     3061 ttccgctgcc gccattgtgc cgctctatgt cggtatggcg aaccatgaac aggccgatcg
     3121 tctggcaaac gccgtgcgca gtcggttact gacacctggc gggattctgg caagcgagta
     3181 cgaaaccggt gaacagtggg ataaacccaa cggctgggca ccgttacaat ggatggcaat
     3241 tcagggattt aaaatgtatg gcgatgacct tctgggcgat gaaatcgcgc gcagctggct
     3301 gaagacggtg aatcagtttt atctggaaca gcacaaactg atcgaaaaat accatattgc
     3361 cgatggtgtt ccccgcgaag gcggcggtgg cgagtatccg ttgcaggatg ggtttggctg
     3421 gactaacggt gtggtacgcc gtttaattgg tttgtacggc gaaccataat atttttacag
     3481 ccagccgcta acttcctgct ggctgtaaaa ttatcctctt caggaggaga tatttaacat
     3541 cattgccgcc tgggtgcgat ttttcacttc cagacggcga tacagggatt ccagatgcgc
     3601 ttttaccgtt ccggtactga tattcagcgc tctgccgatc tccttatttg attcgcccgc
     3661 cgctaacatg gttaaaatct cccgctggcg ggcgcttaac gatttgagat ctttaatgtc
     3721 cttttccggc gtcgtccgcc agtctccagg cagaaacatt atccccatcg ccgcactatt
     3781 taccgccaac gcaaatgtct cgacggttga atcacgaggc acaatggcca gcacattaaa
     3841 atggataact tcctgtaacc accgtttatt gcagtccgtc gccgtaatta acaccttaac
     3901 ctcaggaaat tgcaccacgg ttttttgcag caaccagtag caaaactcac catcctgatc
     3961 gccatcgagc ataactaagg cttcagggta actttccagc ttttgccata actcgtctgc
     4021 ctgactggcc ccctgaatac tcactcctgg aatacgctgc tgtaaactga ttttcattcc
     4081 atgaataaat attgactgcc tgtcaaacat gactatttgc ataactgaat ctccacctga
     4141 atacgttaaa aagacttaag tagtggaagg gtattacccg cgagaaaaaa taagaattcg
     4201 ccatttggcg gtggccattc tacagagatg gcgcgtagaa aatagttacc gatataaata
     4261 gttacagcta aacgcctgaa attacatgtc gagggcacta tttaaaacaa ttttgaggat
     4321 ttccttatat tggtggttag tacgcatgca attaaaaatg aaattccgcg accacaagcc
     4381 aaaataacaa atggcaatga gacaaaaata agcacaaata gccaatatgt cctctattca
     4441 ctttaaaggg aatcgctgaa aaatacgctc tgtttaaggg gattcacctt tctcagaaag
     4501 ctattccgcc cttttcctgc tgagaaatcg ccacattcgt catgacaaca ttgtgaaacc
     4561 cggcattaga tgttagaaaa aataataaac aatgcgatat gcgcgttact gtttcgctgt
     4621 gaacaacaat cggtcaaaga aatggataaa attcacgcaa tgcagttgtt catcaaagtc
     4681 gcggagctgg aaagtttttc ccgcgcagcg gatttctttg ctttgccaaa aggaagtgtt
     4741 tcgcgccaga tacaggcact ggaacatcaa cttggcaccc agcttctcca gcgcaccacg
     4801 cgacgggtca aactcacgcc agaaggcatg acctattatc aacgagcaaa agatgtgttg
     4861 agtaatctca acgaactgga cggtctgttt cagcaggatg ccaccagtat cagcggtaaa
     4921 ttacgcatcg acatcccgcc aggaatcgca aaaagcctgt tactgccgca cctgtcggaa
     4981 tttctctatc agcatccggg aattgagctg gaactgagta gccatgaccg tccggtagat
     5041 attcttcatg atggttttga ttgcgtgata cgcactggcg cgttaccgga agatggcgtt
     5101 atcgcccgtc ccctcggcaa actgaccatg gtcaactgtg ccagtccgca ctatctgacg
     5161 cgctttggtt atccgcaaaa ccccgacgat ctcacttcac acgcaatagt gcgttataca
     5221 ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca gcgttaatgg cgtccagtgg
     5281 tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa actatctcgc cgccggtctt
     5341 gccggtctgg ggattattca gatcccgcgc attgccgtgc gcgaagccct gcgtgccggg
     5401 cggcttattg aagtgttgcc aggctaccgt gccgagccgc tctccctttc gctggtttat
     5461 ccgcagcgtc gggagctttc ccggcgtgta aacctgttta tgcagtggct ggctggcgta
     5521 atgaaagagt acctggactg accgactata ctttttaaga atgaccacga cagaaggaca
     5581 aaagagcgga tgacgcagga aaacgagatc aaacgtccca cccaggatct ggagcacgag
     5641 ccgattaagc agttggataa tagcgagaaa ggcggcaaag ttagccaggc gctggaaacc
     5701 gtcaccacca ctgccgaaaa agtccaacgc caaccggtta ttgcgcacct gattcgcgcg
     5761 acagaacgct ttaacgatcg gctgggtaac cagtttggtg cggctatcac ctatttctca
     5821 tttttgtcga tgatcccgat tttgatggtg tcgtttgccg cagggggctt tgtgctggcc
     5881 tcccatccga tgttgctaca ggatatcttc gacaaaattc tgcaaaacat cagcgatccg
     5941 acgctagccg ctacgttgaa aaacaccatc aacaccgccg ttcagcagcg tacgactgta
     6001 gggcttgtcg gcctggcggt ggcgctttat tccggcatca actggatggg taacctgcgt
     6061 gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct cgccgcaaga tcaggagaag
     6121 ttctgggtaa aatatctgcg tgattttatt tcgctgattg gtttgttgat tgcgctgatt
     6181 gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc aaatgattat tagcgccctg
     6241 cacctgaaca gcattgagtg gctgaaaccg acgtggcggt tgattggcct ggcgatttcc
     6301 atcttcgcca actatctgct tttcttctgg atcttctggc ggttgccgcg ccaccgtccg
     6361 cgcaaaaaag ccctgattcg cggaacattt ctggctgcta ttggttttga agtgattaaa
     6421 atcgtgatga cctacaccct gccatcgttg atgaaatccc cctctggcgc agcatttggt
     6481 tccgtgctgg ggctgatggc atttttctac ttcttcgccc gtttgacgct gttttgcgcg
     6541 gcgtggattg ccaccgccga atataaagac gacccgcgaa tgccggggaa aacgcagcct
     6601 taaaataggc cggatgcagt tcaaaacgcc ttatccggca tacgaattta ttcggcctgg
     6661 ctccccgtag gccgaataag atgcgccagc atcgcatccg gctataatgc gcacataacc
     6721 tcttgaaact ctttcccaga gcctcttcag ccatctattc aggagcaaac aatttcatgc
     6781 caactcataa ccccagcata taaatccagt tggtaacttt tatttaacct gaaaccagtt
     6841 ttatccacta tttataaaat tatgtgaagc atttcataga agaaaaatca ctggcctaaa
     6901 cattatcccc tttttgcctg gtttttgacc atttccgcga tttgttacac attgaaatat
     6961 cacttttgct gtgcgtaata tggctattcg ttagccaaaa aataagaaaa gattatgcaa
     7021 gcaacagcca caacactcga ccacgagcaa gaatacacgc cgatcaactc gcgtaataaa
     7081 gtccttgtcg cctctctcat tggcacggcc attgagttct tcgactttta catttacgcc
     7141 actgcggccg ttattgtgtt tccgcatatc ttcttcccgc agggcgatcc tacggcagca
     7201 acgctacagt cgctcgccac cttcgccatc gccttcgtcg cgcgccccat tggctctgcc
     7261 gtttttggtc attttggcga tcgcgttggg cgtaaagcga cgctggtcgc ctcgttgcta
     7321 acgatgggga tttcgaccgt ggtgattggt ctgctgccag gctatgccac gattggtatt
     7381 ttcgccccgc tgctgctggc gctggctcga tttggtcagg gtctgggctt aggtggtgaa
     7441 tggggcggcg cggcgctgct ggcgactgaa aacgccccac cgcgcaaacg tgcattgtac
     7501 ggctccttcc cgcagctggg cgcaccgatt ggcttcttct ttgccaacgg cactttcttg
     7561 ctgctttcct ggctactgac cgacgagcag tttatgagct ggggctggcg tgtgccattt
     7621 atcttctcgg cggtgctggt cattatcggc ctgtatgttc gcgtgtcgct gcatgagtcg
     7681 ccggtgtttg agaaagtcgc taaagcgaaa aaacaggtga agatcccgct gggtacgctg
     7741 ctgaccaaac atgttcgcgt aaccgtactg ggtacgttca ttatgctggc aacctatacg
     7801 ctgttttaca tcatgacggt ctactctatg acctttagta ccgccgccgc gccagttggg
     7861 cttggcctgc cgcgtaacga agtgttgtgg atgttgatga tggcagttat tggttttggc
     7921 gtgatggtgc cagtcgctgg attactggct gatgcctttg gtcgccgtaa aagcatggta
     7981 atcatcacca cgctgatcat cctgttcgcg ctgttcgcct ttaacccact gctcggttct
     8041 ggcaacccga ttctggtttt tgccttcctg ctgctggggt taagtctgat gggtctgacc
     8101 ttcgggccaa tgggtgcgct gttaccagag ctgtttccga cagaagtgcg ttacaccgga
     8161 gcatcgttct cttacaacgt agcgtcgatt ctcggggctt ccgttgcgcc atatatcgca
     8221 gcctggttgc agactaacta cgggctaagt gcggtggggt tatatctggc ggcgatggct
     8281 ggcttgacgt taatcgccct gctgctgacc catgagacgc gacatcagtc gttgtaataa
     8341 gagtgaaaag cctgatgcgc tacgcttatc aggcctgaat ttccatacaa tatattgaat
     8401 tcgcaaggat ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagcaca
     8461 ctttgccaac aatctaaatc tgtttaattg ccggatgtca gacatccggc aattatcttc
     8521 acttcttcat ctgcgacaaa atcgtccggc actgattagc ctccccttcg gaaggtgaga
     8581 tcagtgccag caacgctgcc gctggcgtaa ccagcgttgc cagtgccgca gcaacagcac
     8641 cacgggcaat caacggcccg gctttcaccc cagcctgagg atttttaaac gtcccacgca
     8701 catacagcgg cgaacgcagt gtgataatcc gaataccttt actctccgga tcaatagtca
     8761 aatccagctg ttccgaagca aaacttgccg tgccggtaac gttaatcaac gcgttctcag
     8821 tatcgaaagc aaaaatctgc gggcgtgcca cgccgttggc aatattcaga ttcgccgccg
     8881 cgcagttcac ccgcacctca tcgtcaccaa atatcgcacc gacaatgtag ttgccgacat
     8941 tcagcccaac aatctccatc aggttgcggc tcaccagccc gtcattcatc aacagtttca
     9001 ggttgccgtt actgttgcct aaaagtgccg ccaccgagtt accgctaccg cgcagttccg
     9061 cgtcaccgtt catttccccc agcgtcttct gcatcagttc cacatcgggc atcagttctt
     9121 tcagtttcag tcgacgagcc tgaatatctg cccgcccctg catcggcttt ttatcgcctt
     9181 ccagatgaat attcgccgca atgctgccac ccgcaatgcc aaatttcagc ggttgcaggc
     9241 gcaagtcagc atttttgagg atgatatgag tagaaagatc gctaatcggc aggctactgc
     9301 catgctcaat gcgccgccct ttgaagcgaa catcggcatc cataacgtcc catttatcgg
     9361 tttcgaagcg gtcataaggc agcactttgc ccgcaggctg aacgcttttt tcgcccttct
     9421 tctgttcaga ccgtttcgac ttttctgccc ctttcccgga atcaacgcca atcaacggtc
     9481 ccaggtccgc cagccgcaat tgccgcgact cgacatcacc ttccagtttt ggtcgtggct
     9541 tgccggtggt gtagaccaga gaaccgtgga tatcgctatc gccaattcgc ccattaaagc
     9601 cgcgataatc aaagaccgac gatttttcag tgtcgatttt cgctaccagc cgaccatccg
     9661 tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga tcacccagtg
     9721 aatcgccaga aaatttaagc cgtaaatcga caccgcccat cttcattggg tcattcacga
     9781 cgccatcaaa agcaacacgg gtattacctg aacggaaatc agcctgtacc ggaaacggcg
     9841 tcccttcgcc acgcagcgcc agcataccgc ctatttttcc cgtacccgtg agcggttcac
     9901 cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt tctttatccg
     9961 ctttaccttt cgatccagta acttcgctga acggcagcgg cttgcctaag ggatcaacaa
    10021 aaatctccag atccgctttg cttactttgt catcaatggc gatccgccct tgatcgaaaa
    10081 gaatattatc cagccgaaac gaccatgccg acggctttgc attcgcgtct ttgttatcat
    10141 cgttggcgag attaaacgtc cagttattgt tcttttcaga gaggcgaatc aggcgcgcgt
    10201 cgggcttttc gagcttgatc cacggcagcc agaccgtttt ggtcagcagc gccagcgggg
    10261 ccagcgttgc ttctacgcgt ggcaaatgca ccatcgtgac ttcgggaata tccggtggat
    10321 tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg cacccagctg cgccagccag
    10381 tttcttgctt ttgccgctcc cacaccacgc ccagatcgcc acggatagcg aacggacgat
    10441 tcaactccgc agagactttc tggttgatgg tcggtttgag tcgattccag tcaaaagttg
    10501 caatcaaaat gatcgccacg acaatcaaca acaagaaagc ccctgaaatc gcagcggtta
    10561 ttttgcctgc cttgctcatc gttcgcttcc tcatattctt cctgtgccag tcctaaagat
    10621 agtccagcca ggcggaaaat gaggcagctt atagcgccag aaccgccgta ttcagcgttt
    10681 ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc gagttctgta
    10741 catcacgcca ctcttccggc gtttctacgc cctcgacaat caccccgcga caatagcgat
    10801 tcattagatg taaaagctga gaaaagagtg tgcgtccttc cggcgactga cgcagcatca
    10861 caaacagttc tcgcgcgatt ttgatgtagt cataacgcac ttcacttagt gcagagaaat
    10921 ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc aaattcacac atcgaggcaa
    10981 aggttgaatc tttcggcaga cggatatgct ccaccagttc gaaacgcagc cagggaagac
    11041 gctcaatctg gcgcaggatt tttggttgct gacgcagggc gatgagcgta ggaccatcaa
    11101 tattgaccga tgccagcagg ccgtgctcta taaagaagtc ggctttttgc gccagcaaat
    11161 caatctgctc tttcacaacc tccatccgat ggctgacggt gatttcagta aaatagcgat
    11221 ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac cgccgttaat agctccacgg
    11281 ccattaaccg cccgcatgtt tgatagatcg gctgccaggt gtaagcacgc tcacactgca
    11341 accaaaaacg ccgttcctgc aagctctcga tgcttgcttc agggttgctt attcgctgga
    11401 taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt gatgcagccc ggactcgaca
    11461 aagattatcg gcgcgcaagg gcagaacttt accgcctggc cggggtaaat ttccccactc
    11521 cacaacggaa tctttcagga atacgttctg gctcaaaaaa ataatggaac actgttttaa
    11581 tatggttgac cagcaaacca ccacagcgca aactaacgct aattttttac agatcaggtt
    11641 cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc tttccgagaa
    11701 aggcgcggac gttaagcgcg gtttcggcgg cgataccctg aacacttccg tctatatcgc
    11761 ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta acggcgctgg gaacggacag
    11821 ttttagccag cagatgctgg acgcctggca cggcgagaac gttgatactt ccctgaccca
    11881 acggatggaa aaccgtctgc cgggccttta ctacattgaa accgacagca ccggcgaacg
    11941 tacgttctac tactggcgta acgaagccgc cgccaaattc tggctggaga gcgatcaatc
    12001 cgcggcgatt tgcgaagagc tggcgaattt cgattatctc tacctgagcg ggattagcct
    12061 ggcgatctta agcccgacca gccgcgaaaa gctgctttcc ctgctgcgcg aatgccgcgc
    12121 caacggcgga aaagtgattt tcgacaataa ctatcgtccg cgcctgtggg ccagcaaaga
    12181 agagacacag caggtgtacc aacaaatgct ggaatgcacg gatatcgcct tcctgacgct
    12241 ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg cgcgcaccca
    12301 taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc tggtgtccat
    12361 tgctggcgaa gggttagtgg atgttccggc ggtgaaactg ccgaaagaaa aagtgatcga
    12421 taccaccgca gctggcgact ctttcagtgc cggttatctg gcggtacgtt tgacgggcgg
    12481 cagtgcggaa aacgcagcga aacgcgggca cctgaccgca agtaccgtta ttcagtatcg
    12541 cggcgcgatt atcccgcgtg aggcgatgcc agcgtaaacg cagcatatgc aaaaggctgg
    12601 ataaagagtt acccctctat ccagctcatc cataaatacc tgatggttgt gctaactcat
    12661 caccatcagg cattactgtg caggtggaat atccgtcact tcaggatgta cgtcatccgt
    12721 ggcgacagag gtggtcgcag cggcggtaga tggggccatg atttgatccc aggccgcctg
    12781 caacgctttc atgttaaatt ccggctcgcc tttcggctgc agcagtatta acgccatatc
    12841 attcgacaac tgctgacgca gatcctgatt taacatctcc acagtcaggc tgttaaggaa
    12901 atcctgccgc agtttctgat actgctccgg cgcgatatcg acgacctgat tttgcaacga
    12961 acgcatccgc tggcccatca gaatatcggt atcagcccgt gcataggcgg caaacagttt
    13021 ctgcagctcc agtttctttt gcgccactaa cgcattgaac tcttcttccg gcagaccttt
    13081 atcgcgaacc ttcgccagtt cacgcgccac cagattaagg ttgctgttca gcttgtcgtt
    13141 tggtgattcg atgttgatgg cacactgcgc acgcagatac agcacacggc agtcaaatcc
    13201 aagaccgatg tctttgctgt tactggcgct taacgcttgc tgaacatgcc agaacagcgc
    13261 ctcacgggcc aggtccgcac gccagtagcg cagcagtgcg gctgattcac gaatcggctg
    13321 ccacggcgta tcccacatga tagataaccg gtcctgacgc accgcgtcag tcataatact
    13381 caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga gccggtgttt cacgtttgcc
    13441 tttcagttcg ccaaacgttt tgttgatttg gtcgacgacc gagcgcgcat ccacgtttcc
    13501 caccaccagc agcgtcattg catccggggt gtaccatttc tgatagaaat ctttaatttt
    13561 ttccgcttca acgggttgtt tcagcggatc ggcaggatcg tgacctaaca aggttgaccc
    13621 tttcagacga tagcgccacc agccctcttt agtatcggca ggccaggttg ccaccatgtc
    13681 ctgactttgc agcgcgtggt tgatggtttc tggcgtgata gtcagtttgc cagtggcatt
    13741 tgccagataa gagagcgcct ctttcagcaa atcgttacgg ttattgggca aactcagatt
    13801 aaacagcgtg gtgtcataag agacaattac cggcggcatc gggcgtttag ggtcgatccc
    13861 ctgctgccac aatgaacgcg cctgtgctgc gtcaaggcca ccgctttgcg ttagcgcaat
    13921 acgagggatg gcgtgactgt aaccgctctg ttgtgtactt tcggcgagcg aaccggtatt
    13981 aaccagcagg cgaatttcaa cacgatcgct gggacgctgg ggggtggtca gcacttgcca
    14041 ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga tcaggctgga gcgcatctgc
    14101 ctgcacatag ccagcagtgg ccatcatcag caaaccgccc gctaaaagtc gaatttttgt
    14161 gccctgcatg tgaacccctg atcaactatc ctggtaataa aaaaactgcc cgttgtcggt
    14221 cagtctaaat gacgttaaaa acacttcgtg ttagaccgca agaacatgaa aatgtcacgg
    14281 aagaagtgaa ataaacctga actcacccag gtggtaagtt caggcacagg ggtcaattat
    14341 gcgcaaacac ccgcactcgg ggaagggagt gcgggcataa gtgatgagat taagaggata
    14401 attcgtgcgt tttgccatcc ggcgcacgat tattcagcgc atcgtccagt tttttgtggt
    14461 ccagttcttt cacccactta gcaacgacaa tggtcgctac gccgttaccg accaggttag
    14521 tcagcgcacg agcttctgac ataaagcggt cgataccgag gatcagcgcc agacccgcta
    14581 ccggcaaatg gcccaccgca gagagcgtcg ccgccagcac gataaagcca ctacccgtta
    14641 cccctgccgc ccctttagaa gaaagcagca acacgattaa cagcgtgatt tggtggacga
    14701 tatccatctg actgttagtg gcctgggcga taaacaccgc cgccattgtc aggtatatcg
    14761 atgtgccatc aaggttaaac gagtagcctg tcgggatgac cagccccacc accgatttac
    14821 ggcagccgag tttctccatc ttgtcgagca tacgcggcag cgccgactcg gaagatgaag
    14881 tccccagtac aatcagcagt tcttcacgga tgtagcggat aaatttgaag atactgaaac
    14941 cagtcgcttt agcgattgaa cccaatacca gcaccacaaa caggatacag gtaatgtaga
    15001 aacagataat cagctgcccc agttgcacca gtgtgccgac gccgtattta ccgatggtaa
    15061 acgccattgc cccgaacgca ccaataggtg ccagacgcat gatcatattg atgatgccga
    15121 agatgacctg cgagaaactt tcgatgacgt taaaaatcag ttggcctttg ctgcccagac
    15181 ggtggagcgc aaaaccaaac agtacggcaa acagcagcac ctgcagaatg ttaccgctgg
    15241 caaatgcgcc aatgacgctc gccgggatga catccataat gaaggcgaca atgccctggt
    15301 ctttcgcctg atcggcgtaa accgctaccg ctttcgcatc aagcgttgcc ggatcgacgt
    15361 tcattccggc accaggctgc acgacgttaa cgatgataag accaataatc agcgcgatgg
    15421 tactgacaat ttcaaagtaa agcagtgcga ctgcgccggt acgaccgacc gccttcatgc
    15481 tttccatgcc cgcaatgccc gttacgacgg tacaaaagat gacaggagcg atgatcatct
    15541 taatgagctt aacgaagccg tcgccaagcg gtttcatttg ctcgcctatt tcaggataga
    15601 aatggccaag gagaatacca atggctatcg ctgtcaggac ctgaaagtaa aggcttttaa
    15661 acagagaggt tttcataggg tgtcctttag taaaaccaca ggtcttgtaa ggttatgggg
    15721 tacctgcggc cttaaaataa cacccagaca acatcacaga aatgtacctg gatcataatt
    15781 gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa ccagcacttt ttaaagtttt
    15841 gtaatcagtt tggggtagct acttttcttc caggtaactc tcttcgaaga tttcaatagg
    15901 gagtggacga gcaaaaagga agccctgggc aataccaacg cccgcttttg ccagccagtc
    15961 gcgttgtgct tcagtctcca cgccttcggc aatcatttgt aagttcaggc tctgcgccag
    16021 catgataatt gcagcaatca tgctgctatc ttccggcaag ccttcaacaa acattttgtc
    16081 gattttcaga acgtcgattg gcaacgattt catatgctgc agctgacgca gccctgcgta
    16141 gcccatgccg aaatcatcca gcgccacccg aactccggca ttacgcagcg gacggaggat
    16201 tgccaccgca gcatgagggt cgtcaatacg tcggctttct gtcacttcca gaatcagtgt
    16261 tcccggctga atgcgatagc gggttaacag ttccagcata tccgccacca tattcgggtg
    16321 catcagttgc agcacagaga ggtttaccga caagggcagc ataatgccgc gctcttgcca
    16381 ggctgcaagc aatcgacagg actcttccag cacccagtga ccgacggtaa ccatcagccc
    16441 acagcactca atgcgatcga ttaagccatc cggcaggtcc caactgccat ccggttgctg
    16501 gatacgcagt aacacttccg cactgaccag tttaccgctg gtcatctcga cctgtggctg
    16561 taaccaaata gcaaactgat gattttccag tgcattaagg atatcgctct cttccgtcaa
    16621 ccgcttctgg gcggcttcca tctgctgcgg atcaaagaac tgaatctgat tcttgccttt
    16681 atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa agctgttcgg cggtgagatc
    16741 gccgtagaac atcgccacgc taatgctaca gtgcggacgg agttgaatac gttcaatcgg
    16801 caggcgctcg ctcatgatag tgagcacttg ctgacccaaa gtgattgcgt gccacggttc
    16861 atgtacaccg ttggcaatga cagcaaagtc ataaccgcta atctgcgcga ggatcatacg
    16921 tggcgacagt accgatttga gtttttccac cagcgtcagc agcagaattt ctcgttgcgc
    16981 ctctttcagc acgcccgcag tatcacgcag agtttcacag gtgatgatca tcagcgcggt
    17041 ggtttgttta cgcgcgacaa cctgctccag catctccatc agcaaggctt tgttcggcaa
    17101 atccgacacc gggaagcgca tcgcattttc gttctgttct tcataatggc gctgcagcaa
    17161 ttgctggttg aggttgtaac tgcgcaccaa cataccgatt tcatcgtcct gatgcagacg
    17221 cggtaatgcc agttggtgac caacaagctc ctgggctggg atggcgttaa gttcgcgagc
    17281 aatattgcgt aacggatgca aaatcaggcg gttaatgcac cagctaatcg ccaccgtcaa
    17341 tattaacgac aaaagtaagt aaatggtcac taacgttgag agggtgctca tcacgaactt
    17401 atacatacgg aaggaatccg cctgtagcac cagataggca attggctgcg gatttgccgg
    17461 acgttcgagc gagtagacgc ccagcgagat ttgaaccggt agctcaaaca ggcgagtaac
    17521 cattaccggc accggacgct ctggaataaa acttttacgc agcgcctgga actggttagg
    17581 cagcactaca tcggcacggc tgaccacgcc tgccggttta atgctggcaa gaatagcttc
    17641 cgcttcggga atatcacctt tcaaaatggc tgaagataaa ggttcgcgga cagagcgagc
    17701 aatgctttcc agttgcgtag ccgtgttata gcgattctgc tggaccagat ggaacagcaa
    17761 aacggtgcaa aaaataaaaa cgaacaccag gacaacggct gccaccattg ccatctgctt
    17821 gattgttaac gagcgactta cgcgcaaatt gtctcccccc ggaaatccca gattgccgcc
    17881 cgagtatacc cgatcgcagc ggcattaaga gaggcgctat ctgaaaactt accagtcggc
    17941 gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc tgccatccgg cggcggaata
    18001 acgtacgtag agcaggaaat ggctgggggc gtaatctttc gcctgctgga tatcaattgc
    18061 cgtgccgaca aaccagttgg aagtaacacg tcgttcaagt aatgcccgcg ccgtatagcc
    18121 gaagccctga ctgctgccgc catcgttgga ttgccgcgca gcttcttcct gccagtcggt
    18181 cgggatcaga ttcatcagcg gataacgcgg catggttttg gtgcgtgaat gcgaccacga
    18241 gccagacgcc cccagctccc acgaccagtt ttccgtgcgc tcccgccaca tcaccggtat
    18301 ggcaaacaac aggtattcct gcgggctgta gtaaccgccc taaccgagtg agtagccact
    18361 caggtctttg tcgtaatgcc agatcatgtt gttcaggccg attgtgacgc ggcgattgtt
    18421 ctggttgatg accttatagt aatagcccgt catccagcgc acacgccagt tatcttcgac
    18481 atttttaccg gttaactggt cgccactaag cgatgcccag acgccgtttg cttcaccttt
    18541 atcgtagctc aggcttagcc ccacgccgtc ggcacgcacg ccgccccatt ttttcccggt
    18601 attgctcggg gagtcttttt gcccaccaaa ggccagcaaa gaactggaga tgggacgacg
    18661 gtgggcgtta acggtgtaac ccagcggccc gatatcatcg ctgtaactga tgccgccgac
    18721 cacatccacc acgttgaagc ccatcggcgt ggtgccgata tcccagctcc agacgtcatt
    18781 tcgccagccg accgccacgc tggcaccgga atccgactgg ctgcggttgc cgctacagtc
    18841 ccgtaatgta caggtgcccc agttgtcatc ccatttgcca tcggcattag tggagaaact
    18901 gccgacgttc atattgacga aatcactgcg aaagaacatc cgcccgtcag aatacggcgc
    18961 atccacctgc aacatggtag tgtgcgcttt cagatcggag taaccaccgg tgccgctcga
    19021 accccaataa tcatgctcga gggtgacgtt aagatcctgc tggcgataga ggtccgccgc
    19081 atcgctgcgc acgccgcgtt tcagccagtc atctttctcg tcgttacggg tcagtcgggt
    19141 aaaggtgtcg ttatcctgcg gacgcgtcgt agtcacaccg gatacgacca tggcgtcttt
    19201 gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct tcaaacttcg caccgtcgcg
    19261 cagcaccatc gcgctttcca tcgacggtgg ctgagatttt gcctgcggga tcaacttatt
    19321 aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc tgcgccagcg ccacgcgccg
    19381 ctgtgtgttc agcgaggcgt tatcagtagc gggcagtttc gccagctggc tacgtgccgc
    19441 cgctgtgtca ccggcagcaa tatccacttc cgtcagacca agaatggcat cggcgttagt
    19501 tggctcccgc gtcaggacat tctgatatgc agcgcgggcg gcggtgtaat cacgtcgctg
    19561 cagcgcccag tcagccagcg tgaggtcaat acgcgtggaa ggtggttgct gacgcagcat
    19621 cgcttccgct tcagcttctt tgccgctttc tcgcaggcga ttagcggttt ccagcacctg
    19681 atcgctttgc agtcgattaa ccagctcctg aatattgctg ctccactgcg cacgcggcag
    19741 gctgttgata tgcgccagcg ccgctctgtc ctagtcatga ccagagaggt acagcccgta
    19801 agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc agattgcgca ttaacgtatc
    19861 ggcctggctg cgttgtccgg cctgccagag atcctgcgaa agtcggtaag taatccatac
    19921 gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct gccgcctgcg cccatttgcc
    19981 ctggttttcc agtgcctctg cctgctgtgc cagacggtcg ttttgcaggc tgcgttcgat
    20041 atcatcaatg ctacgccgct gactggcaga gagtgaggcg ataaacgctt cagctttttc
    20101 tggcgattgc tggcggtaaa tatttgccag cccgcgcacg gcgttagtgt tgccgctgtc
    20161 catacgcaag gtctgctgat aataacgttc ggcggcggga taatcttttc gcgccatcgc
    20221 cacatcgccc agccccagca ctgcataact gtcggtgtta tcgacattac gcgcctgctg
    20281 gaacaggcgt tctgcccggt caggattatt ggctttcagc gcagcatcgc cctgctggat
    20341 cgccagccag tagcggttta ctttcagcag actgttccat ttgtcgttgt tgctgctgtg
    20401 cggatccagt gcgagggctt tttccagatt cgccactgca ttggcgcgat cgcctttctg
    20461 tgaatatgcc tggcccagcg cccccagagc ttcactgtct ttcgggtttg cccgcactgc
    20521 ctgttgtagt tcgggaatgg ctttacccgc cataccagag tccaccgccg ctaaaccttg
    20581 cgcacgagcg cggaaagcag gatcggccag ctgtttttgc tgttctgcca gttgcgattg
    20641 cgcagccgcc acgctatcgc catcactaaa gatagaaaga tattttttca gcgccgacac
    20701 actggcatca ctgacgggca tgtctttaat ctgcccgtac cagattttag aggccccttc
    20761 gcgcccggcg ttcgatttcg ccatctgttc caggacggca aaaccttcgt cacggcgatc
    20821 gctactaaac agcaatagcg ccagattgtt ttgcaggccc gtattgcccg gtgcatccgc
    20881 attgatgcgt tttaactgat taatcgcttc gccacggcga gccggaattt tcgccaccgt
    20941 actccagtac tcgacagcaa tgtcaccttc cggcggcgca ccgttgaaca gtttgttgta
    21001 actcgccaca gcttcttccg catgaccggt agtcgcctgc aatcttgcct gttgcagtgc
    21061 ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc gacgatttat acgcatttga
    21121 actcggcgct aactgcgaca gccgatcgag ctgtttttgc gcgccatcaa tatcgccctg
    21181 acgtaacaaa gaacggaaac gggcggcaac gacgtccggg ttattcggat caataagttc
    21241 cagccgatac aacgactgct gcaccaggtc ttcacgatgg gtcgcttcgc ctaaccgaac
    21301 ttgctccagc aactgttgct gagcggttgg tgctgcctcg accatcggca tgacggccag
    21361 accgagggaa agcgtgaata tgtttagtgt gaatttgcgc attcctggcc ccagtcaggt
    21421 aataactcac cttttgtcga gaagcggaaa cggtgttgat cccagccttg tccaaacagg
    21481 gtcagcacat agttgtaata ggcatcgctg ccgggaaagt tatcggccac gcgctggcgc
    21541 tgaacggcct gcgcatcacg gttttgtaaa aagggcagca tggcggcaga aaaaccgacc
    21601 ggtcctttac cctgcgcttt ccccgtagcc acatccactt tttccggcgg ataaccgttt
    21661 ttctcagtga atgtcgccat cggtttaaac cggttgagca tccgcgcttt ctgcggatcg
    21721 ctgtcaggca tcatgcctac ccacatgtaa acgcggatag cgtcgtagct gctgatcaat
    21781 gttttttcgg cttttagctg ccagcctttg tctttctcat agcgcaccca gtctggcgaa
    21841 aagcctttcg gggcggtttc cagcaataaa cgttgattgg tttcgcgcag cgtagtccac
    21901 ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg gcaggtagct ggggttaaaa
    21961 cgccagctgt tatcctcagc aaaacccact ttgcccggta acaacatgga acccagccca
    22021 ggcaccgtca ccacttcctc ccgcgcaata cgttttagca acgcgctgcc gatgtcggta
    22081 taacgctgct ctttccacaa acgccccgcc tccagcaacg accaagccat ccagacatca
    22141 ccatcggagg ccgaattgct gtccagcact tcccacttac tgttctcttt cttgccccac
    22201 agccaggcgg gcaaacgttc ttttaaagaa ccctgagcga gattgttctg cgtccagtcg
    22261 agaatattat cgaaagctgc acggtcgtta gccgccaggg caaagaacat gccgtaactt
    22321 tgcccttcag aggtggtgat tttgcgcgcg tcgctggggt cgatgacgcg cccttcctga
    22381 ctgatgtaat cctttttaaa ctgctcccag gcaggccagg tacaggctgc ctgaacacta
    22441 aaggcagcca acagcagcat cgtcacgagt ccactacgca acacattcat cttcaattac
    22501 tcgttatccg ggttaagacg acgacggctg ataatacgca gcaaacgcca cagtacccat
    22561 gccagcaata tcacactgat agccgccagc accgccagca ccgccagcac cgccagcacc
    22621 gccagcagaa tcggatggtt tgccagcgca taccacaagc gctcgaacca cggcagatga
    22681 cctacgtaat aaacgtcgcc aacacgtagg ctgttgatac cggactcgcg gatcaccgcg
    22741 accgaaccga acatggtggc gcgtttgccg ctatcgttca ccgcatcgtt aagcatttca
    22801 taaccgcgtg ggctatctgc cagcagcgca atcacgctgc gctggtcgtt atacggcgac
    22861 tggaagccaa tcaccgccgc catcgcaccg gaagaggtca gcgttgactg ggtttctgcc
    22921 gcgcgatcgc tctcgtccgg cacaatgccg gggaacgggg tctggcgcat cggtgttttc
    22981 acccagcttt cggtcgcctg caccaatagg tcgatctgct tatcgtcttt cagtttgtcc
    23041 gggataccac cgatgatcat gatgtcggca tctttgccct gaatggtgct gccatcatcg
    23101 gtcaccgtca gattaatcgc cgggaagccc gtctgtgcgc cgataaaacc aacagtattc
    23161 agcaacgttt ccatctgtgc ttcgttaggc gttttcggca tcacggtgat ggtttgcgac
    23221 agatccgcca tccggctgaa cgggaagccc gcgttagcaa aggcgcgtag atccggcatc
    23281 gggatgaagt ggtaatactt cgagaagtcg atggtggagt cgtcaccaat caccacatga
    23341 ttctgcaccg gctggaaggt aatacagtta tccaccgaac cgccaggcat cgggttcata
    23401 tactcaaagt cgaagcgcag ctggttggtc gcgcccagtt tcagcgccgg aatagagaca
    23461 tccgttttgc catccagcaa accttgtaat accggaatcc gcagcagcag gcggttcgcc
    23521 tcctgtttgc tgctcaggtt gaaggattgc aggaactggt tattcaggct gatatccatc
    23581 cgcgaactgt ctttcaccgg cggcatggtg tagcggtaat taatatccat atcaatgccg
    23641 gtactgcgca tcaggtagag atctggcggc aggtttagcg aaacattaat cgctgctggc
    23701 tcaagaccgc tggattgtaa ctgttcttca taggttttca gttcaccaaa ggtgaccgga
    23761 cgatcggtac gcacccagtt cggcgcatcg tacggcttac gcggtagcag cggtttcact
    23821 tcattcacta ccacgctttc accacggaac agaatgttac cctgagcgat acctttcgct
    23881 gcctgcaaca ggtctttgtc gtcacgacca aacaccacca gcagtttgac gtaaggattc
    23941 tgcggatggt taatcatctc aatcaccggg gcttttaccg ccggatgatc gcgcaggaag
    24001 tccggccgtt tgtcgttggt tgcaaagaca atggcattac gatccggcag ttggttatag
    24061 agcaccggga agttctgccc acgccagcca gaacgcgaac caaaccacga ggcgacaatg
    24121 gcagatgctt gttgcagccc aacatccggc gcacccgcaa agaccatcgg caaggtgttg
    24181 gtgcggttat cacgcgggtc aaagaatggc accgggaagt gtgacaggtc attcttcaca
    24241 ttcagggtct gataggtcag atccagtcca ctgctccgcc caacatccag ccaaagcgtg
    24301 gtgctggccg ggttttcgca cacgtcctga taatggccga caaactccag ccgcacacgg
    24361 ttgaagtcgg taataaacag tggattaatg ggcatttgcg ccagcgtttt tttacccaac
    24421 tgttctttgg tcactggcag cacgcccatc agctcatcat tgagataaac ctttaactgc
    24481 gactggacag gcagtaacga tggcgatggc gtgtattcga ggttgagcat cgccttcgtc
    24541 accacttcat cgctgcgcat accaaactca atgctgccgt tcgggttaat gccacgtagc
    24601 accatgctgc ccggcggcgg cgcaatttgc gcaaaggtca gcttcacatc acgcgaagga
    24661 ccgttctgcg ccacgactgg cgcatcagcg ccctgcacgc ccggcatcac ttgccccacc
    24721 tgcggatttt gttccgtctg ggcggctaca gctggctcag cattgatcag tggttgcgtt
    24781 gctggcgtcg cctgcgtcat gaaagagggg aacgcactca tccccatagc cactgcacaa
    24841 atccagaata gttttctttt catcgcgtta tcatcattgt tgagccaaag cctgatccga
    24901 tggttgtgcc gtttcgctcc gctccgggcg gcgcggaata aacgatacaa cccaggaaac
    24961 cagagaagtc agcacacgga atatgccctt caccgaagaa ggcgcaaact ccgccagatg
    25021 gcggtagcca cggaagccga gcttcagaat atccagcaga ctttccagcg gcttatcttc
    25081 cgggtagctg tcctgccaga gcgcccatgt atccgcacgg gcaaacgtac actgcacaaa
    25141 atcgatatgt tgctgggtgg tgagcggcat taatttcagc ccaacttcat tacccatcac
    25201 gcgcgccacc tgggtcggga agacgtattc ctgctgaccg cgtttaagca acagattcac
    25261 tttctgccct tccagaatct gcgcctgacc gttgatcttg atccccaaac caccgtcgga
    25321 gaaatcctga acggtacacg agaagaggtg accatcttcg cgggcaattg ccgcgggcat
    25381 cgtcatctcc acgcggtgcg atcggcgtac ctgtttgctt tctaccgata ccgcaactgc
    25441 gccgccaaga acaatcaggt tgtagaatac ccacaccata ctgacgacca cggtgagcat
    25501 ctcggttggc gggccataga agtagcgcca gatgcctacc gcaacgccca ccaggttgag
    25561 cagaacaagg aagatgtagg gccgcgagat cacccagtcg acgtactctt cttccaccag
    25621 tccacctttg gcggtgacgt taaatttgcc tttgtgcggg ttaatcagcg ccaccagcgt
    25681 cggtggtgcg atataccacg ccagcaccgt ttcgtagatt tcactccaga aagagtggcg
    25741 atatttgccc tggatcttgg agttggtcag gctggcatgg atcatatgcg gcagcacgaa
    25801 cagggcgatc atcaacgctg gcgcatagat gatgtaggca tgaagcagca ggaacgccag
    25861 cggcgcagtc aggaagatca gccgtggaat gcccgacaag aagtggaaca tggcgttgac
    25921 gtagcacagc cgctgagcaa acttcagccc tttaccggtg agcgggttat cgagacggaa
    25981 gatttgcacc atcccgcgcg cccagcgaat acgctgaccg atatgcgccg acagactttc
    26041 ggtcgccagc cccgccgcct gcggaatacg catatacgcg gaggtatagc cacgacggtg
    26101 caaacgcaga gaagtatgcg catcttcagt cacagtttcg acagcaatgc cgccaatttc
    26161 atccagcggc ttacggcgaa tcaccgcaca ggaaccgcag aagaaagtgg cgtcccacat
    26221 atcgttgcca tcctgcacca gaccatagaa cagcgtgcct tcgttcggtg ttttacgaaa
    26281 acgccccagg ttgcgttcaa acgggtccgg cgagaagaaa tggtgtgggg tctgcatcat
    26341 cgccagctgt ttctctttca ggaaccagcc cacggtcatt tgcaggaacg atcgcgttgg
    26401 tacgtggtcg cagtcgaaaa tcgacacgaa ctcgcctttg gcatatttca gcgcattgtt
    26461 gatgttgccc gctttcgcat gttcatgagt ggtacgggcg atatacttca cccccacgtt
    26521 ttgcgcaaac tggcgaaact cttccctgcc gccatcatcg aggatccaga tgttcagctt
    26581 gtctttcggc cagtcgatac ccagcgaggc gtaaatggta tttttcacca cgttgagatc
    26641 ttcgttgtaa gtcgggacaa agatatccac cgacggccac agcgacatat ctttcggcaa
    26701 tggcaccggc tgacgattca gcggccatac tacctggaag tagccgagca ccagcacaat
    26761 ccacgcgtac gtttcagcga agagcagaat aagcccgcac accaggctga ccggatcgtc
    26821 ccagttcagc gtagaggtat atcgccacca gatataacgg caagaaacag tcagcgacag
    26881 cacaatcaac attagcgctg agaagcgccc cggcatccgc cgtacgatca gcgctccccc
    26941 ccacagcagc atcaggaaga taaactgcgc cagcgggtta aacggctgag taacgcagat
    27001 taacgccaga atcagcgaga aggtgacgat gataccgagg atcaaccgcc gcgccccggc
    27061 actcaaatga ccgagctctt ttttctcatc aagatgctgt gttttatggc taacgcgctc
    27121 aggcagctcg ttcatccatt gatggtaacg tccacgaata ttctgcagac ctgaaaatgc
    27181 ccgcctgcgc ggtttcggcg tttctttgcg cgatgcaccg atcagtaacc agcatgtttg
    27241 aatgagataa cggaccgggt ccagcggacg cggacgcgga cgcgaggcgt tgatatgcgg
    27301 atacaggttt ttatgttctg cgcgaatacg ctgccagcgc gggtgctcca gtggaataaa
    27361 aatccaggcc aggatcatcc agaaacagcc gagcgtcgcg ctgaaagccg acgcaccgtg
    27421 acgacgataa tcgcgataac gcccgataag ccgcgcgttg accggcggga taagcaacca
    27481 ccgggtcagg atactcatga tgcactcccg actggcgttt tcagcccgga atagttcaac
    27541 aggcaccagt tcgccagcgt caatatctct tcagccgcca gcgcatcact gcgatattca
    27601 cctactggtt gcttagccgc caggcattca gccatcgctt catcacgatg aatgagcatc
    27661 ggcagtaatc ggcgctggct ttgcaaccaa agctggtaaa tatcgtcctg aacctgactg
    27721 ccaatacgga agttattaat caaaatatgt gcgccatccg gcagcgcttg ctgatgcagt
    27781 cggatatggc agttggcatc cacattgacg attgccagcg agtgatcgca caaactcagc
    27841 agctggtggg ttatctgcga ggcatcacgc ggtaagtcga ttaaaatcca ctggtaacgc
    27901 ccgctggctt ttagttgctg taagccggag caaatatcgc tcagccgggt ttgccagtgc
    27961 tgtggatttt cttgttcttc aatggataac tgaccaaaag gcagcaaatc gagctgcgag
    28021 gtgtagcgca acccagcgtc gcgccagtcc tagccatcca gcatagctct ggcccagccc
    28081 tgacggtggg taaaatcaac attaaatgac aggcgcaaca agttgtccgg gcaggcatcg
    28141 accaccagga cattttctcc caacatttgt aatgaccagg ctaatgcggc ggtg
//
LOCUS       DAZKFZ010000043        28053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000043 DAZKFZ010000000
VERSION     DAZKFZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(151..1014)
                     /gene="potB"
                     /locus_tag="ACHOQW_001698"
     CDS             complement(151..1014)
                     /gene="potB"
                     /locus_tag="ACHOQW_001698"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP9572787.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(998..2134)
                     /gene="potA"
                     /locus_tag="ACHOQW_001699"
     CDS             complement(998..2134)
                     /gene="potA"
                     /locus_tag="ACHOQW_001699"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP9572788.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2384..3610
                     /gene="pepT"
                     /locus_tag="ACHOQW_001700"
     CDS             2384..3610
                     /gene="pepT"
                     /locus_tag="ACHOQW_001700"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9572789.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3659..4780)
                     /gene="roxA"
                     /locus_tag="ACHOQW_001701"
                     /gene_synonym="ycfD"
     CDS             complement(3659..4780)
                     /gene="roxA"
                     /locus_tag="ACHOQW_001701"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP9572790.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            5029..6258
                     /locus_tag="ACHOQW_001702"
     CDS             5029..6258
                     /locus_tag="ACHOQW_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9572791.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6623..6811
                     /locus_tag="ACHOQW_001703"
     CDS             6623..6811
                     /locus_tag="ACHOQW_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9572792.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            6869..7423
                     /locus_tag="ACHOQW_001704"
                     /pseudo
     CDS             6869..7423
                     /locus_tag="ACHOQW_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            7616..7813
                     /locus_tag="ACHOQW_001705"
     CDS             7616..7813
                     /locus_tag="ACHOQW_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9572793.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            7806..8018
                     /locus_tag="ACHOQW_001706"
     CDS             7806..8018
                     /locus_tag="ACHOQW_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572794.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            8008..8472
                     /locus_tag="ACHOQW_001707"
     CDS             8008..8472
                     /locus_tag="ACHOQW_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572795.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            8465..8698
                     /locus_tag="ACHOQW_001708"
     CDS             8465..8698
                     /locus_tag="ACHOQW_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572796.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            8704..9003
                     /locus_tag="ACHOQW_001709"
     CDS             8704..9003
                     /locus_tag="ACHOQW_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9572797.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            9000..10400
                     /locus_tag="ACHOQW_001710"
     CDS             9000..10400
                     /locus_tag="ACHOQW_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP9572798.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            10601..10852
                     /locus_tag="ACHOQW_001711"
     CDS             10601..10852
                     /locus_tag="ACHOQW_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572799.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            10849..11259
                     /locus_tag="ACHOQW_001712"
     CDS             10849..11259
                     /locus_tag="ACHOQW_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9572800.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            11270..11542
                     /locus_tag="ACHOQW_001713"
     CDS             11270..11542
                     /locus_tag="ACHOQW_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9572801.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            11499..11627
                     /locus_tag="ACHOQW_001714"
     CDS             11499..11627
                     /locus_tag="ACHOQW_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9572802.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            11669..11893
                     /locus_tag="ACHOQW_001715"
     CDS             11669..11893
                     /locus_tag="ACHOQW_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572803.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            12145..12351
                     /locus_tag="ACHOQW_001716"
     CDS             12145..12351
                     /locus_tag="ACHOQW_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572804.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            12351..13406
                     /locus_tag="ACHOQW_001717"
     CDS             12351..13406
                     /locus_tag="ACHOQW_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9572805.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            13419..13754
                     /locus_tag="ACHOQW_001718"
     CDS             13419..13754
                     /locus_tag="ACHOQW_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9572806.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            13767..14180
                     /locus_tag="ACHOQW_001719"
     CDS             13767..14180
                     /locus_tag="ACHOQW_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFP9572807.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            14386..14928
                     /locus_tag="ACHOQW_001720"
     CDS             14386..14928
                     /locus_tag="ACHOQW_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9572808.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            15184..15465
                     /locus_tag="ACHOQW_001721"
     CDS             15184..15465
                     /locus_tag="ACHOQW_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572809.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(16066..17526)
                     /gene="phoQ"
                     /locus_tag="ACHOQW_001722"
     CDS             complement(16066..17526)
                     /gene="phoQ"
                     /locus_tag="ACHOQW_001722"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP9572810.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(17526..18197)
                     /gene="phoP"
                     /locus_tag="ACHOQW_001723"
     CDS             complement(17526..18197)
                     /gene="phoP"
                     /locus_tag="ACHOQW_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP9572811.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(18366..19736)
                     /gene="purB"
                     /locus_tag="ACHOQW_001724"
     CDS             complement(18366..19736)
                     /gene="purB"
                     /locus_tag="ACHOQW_001724"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9572812.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(19740..20381)
                     /gene="hflD"
                     /locus_tag="ACHOQW_001725"
     CDS             complement(19740..20381)
                     /gene="hflD"
                     /locus_tag="ACHOQW_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP9572813.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(20417..21523)
                     /gene="mnmA"
                     /locus_tag="ACHOQW_001726"
                     /gene_synonym="trmU"
     CDS             complement(20417..21523)
                     /gene="mnmA"
                     /locus_tag="ACHOQW_001726"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9572814.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(21577..22038)
                     /gene="nudJ"
                     /locus_tag="ACHOQW_001727"
     CDS             complement(21577..22038)
                     /gene="nudJ"
                     /locus_tag="ACHOQW_001727"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP9572815.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(22048..22701)
                     /gene="rluE"
                     /locus_tag="ACHOQW_001728"
     CDS             complement(22048..22701)
                     /gene="rluE"
                     /locus_tag="ACHOQW_001728"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP9572816.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            22873..24123
                     /gene="icd"
                     /locus_tag="ACHOQW_001729"
     CDS             22873..24123
                     /gene="icd"
                     /locus_tag="ACHOQW_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP9572817.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(24237..25379)
                     /locus_tag="ACHOQW_001730"
     CDS             complement(24237..25379)
                     /locus_tag="ACHOQW_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9572818.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(25369..25605)
                     /locus_tag="ACHOQW_001731"
     CDS             complement(25369..25605)
                     /locus_tag="ACHOQW_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9572819.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            25745..26533
                     /locus_tag="ACHOQW_001732"
                     /pseudo
     CDS             25745..26533
                     /locus_tag="ACHOQW_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            26530..27231
                     /locus_tag="ACHOQW_001733"
     CDS             26530..27231
                     /locus_tag="ACHOQW_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9572820.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            27228..27530
                     /locus_tag="ACHOQW_001734"
     CDS             27228..27530
                     /locus_tag="ACHOQW_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9572821.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            27598..27930
                     /gene="emrE"
                     /locus_tag="ACHOQW_001735"
     CDS             27598..27930
                     /gene="emrE"
                     /locus_tag="ACHOQW_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP9572822.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAVIGMMLICAGVLVIN
                     LLSRSTPH"
BASE COUNT         7401 a         6617 c         7216 g         6819 t
ORIGIN      
        1 tgttttttta tagcgatttg ctaaaaggga gaagaaaaaa cgttctccaa aattagattg
       61 caactgtttg attttatgga tagaggcatg ctgtttacta gacagtaaaa agcatgccaa
      121 atttcctatt aaccttttga atataaagca ctaattatct atttctgttg ccttcttatt
      181 cagcagacga gaagcgcgcc agtaaaccag caacatcagg cccattacga tagtcagcgt
      241 aatgctggtg gctgcaccaa acggccagtc acgaatattg aggaactgta ccttgatgac
      301 gttaccgatc agcaggtttt tcgcaccgcc catcaggtcg gacacataga acaggcccat
      361 cgctggcagc atcaccagca gacatccggc aataatcccc ggcatcgtca gtggaataat
      421 gatgcggata aaagtctgta acttgctggc accgagatcg cgcgccgcct caagcaacgg
      481 cttatccagt ttttcgatac tggagtacag cggcatcacc ataaacggta gcagaatgta
      541 aaccagaccg ataatcaccg cactgggcgt gaacatgata cgaattggtg tgtcgataac
      601 gcccagccag agcaaaaact cgttgagata gcctttggtg ctgaggaaaa ttttcagccc
      661 gtagatgcga atcagtgagt tggtccagaa gggaacaatc agcagaaaca gcagcagcgg
      721 acgcaccttg tgtggcagct tcgccagaaa ccaggcaaac ggatagccca gcaccaggca
      781 ggcgagggtg gcgatcagcg ccatattcag cgagtgcaat agcacttcaa aatagagcgg
      841 atcgagcaga cgtgtgtagt tatccagcgt aaagaccatt ttgacaaaac tggcgtcgtc
      901 gcgggccaaa aagctggtgc caatgatcat caggttgggc agaaagacaa acaacacaag
      961 ccaaccgaca atagtgacaa tcactacatt ctggaactta cttgtgttct tcatcagcca
     1021 gtacgacctc ccagctttct acccaattaa tggccatttt ttggtcgaga gagtggtcaa
     1081 agtcaggatc gtcttcattg aagaattcgc tgaccatcac catcttgcca ttttccagtt
     1141 caacaaccga ctccagcgtc atgcctttgt agttacgctc gcgaacgtaa ccaatcagcc
     1201 cttcagcgtg gttgtcgtcg ttaatctctt caacacgtaa gtcttccggg cgcagcagaa
     1261 catgcagttt ttgccccggt tcaacggcga agttaacgta gatattacat tcgcggcctt
     1321 caacgttggc gcgtacgcgc tgctcgtcga gacgttcgat gacagtggcg ttaaacatat
     1381 tgatttcgcc aatgaacccg gcaacaaaca ggtttttcgg ctcttcgtag atttcacgcg
     1441 gcgtgccgtc ttgctcaatg cgaccatcgc gcatcaccac aatcctgtct gacatggtga
     1501 gtgcttcttc ctgatcgtga gtcacgaaga cgaatgtaat gccaagctta cgctgtaacg
     1561 ctttcagctc gttctgcatt tgcttacgca gtttgtaatc cagcgctgag agcgactcat
     1621 ccagcaacaa cagacgaggc ttgttaacca ccgcgcgagc aatggcgacg cgttgctgtt
     1681 gaccaccaga gagctgatgc ggtttacgtt gagcgaaggt ttccagctgc accatccgca
     1741 gggcttccat cacgcggggc gtaatttcag cagcgggggt tttttgcatg cgcaacccaa
     1801 aggccacatt ttcgaacacg gtcatgtggg ggaaaagtgc gtagctttgg aaaacagtgt
     1861 tcacatagcg gttttccgcc ggaacgtggg tgatgtcctc gttatccaac atgatgcgtc
     1921 cggaatcaac agtttccaga cctgcaatca ggcgaagaac ggttgtttta ccgcagccag
     1981 aagggccaag cagcgtgagg aactcgccat tgttgatagt cagatccagc tggggaatga
     2041 cctctttacc atcaaagcat ttgcgaattc ccgccaattg caccagcggt gaaagcgaac
     2101 tcggttgttt attcaatttt ttactctgtc ccatgtaaac gcaacggatg gcttaccgat
     2161 gcggggtttg tggttaacca ccttggtgac tcttaatgag ggcggtaatt ctacggcaaa
     2221 ccgcttgaat cgccaatctt tgttgtgaat tactggctta gctttatatt cattaaggta
     2281 atgctgataa atattcccgc ttgcaggggt aaaagtgacc tgacgcaata tttgtctttt
     2341 cttgcttctt aataatgttg tcacaaaaag tgagggtgac tacatggata aactacttga
     2401 gcgatttttg aactacgtgt ctctggatac ccaatcaaaa gcaggggtga gacaggttcc
     2461 cagcacggaa ggccaatgga agttattgca tctgctgaaa gagcagctcg aagagatggg
     2521 gcttatcaat gtgaccttaa gtgagaaggg cactttgatg gcgacgttac cggctaacgt
     2581 ccctggcgat atcccggcga ttggctttat ttctcatgtg gatacctcac cggattgcag
     2641 cggcaaaaat gtgaatccgc aaattgttga aaactatcgc ggtggcgata ttgcgctggg
     2701 tatcggcgat gaagttttat caccggttat gttcccggtg ctgcatcagc tactgggtca
     2761 gacgctgatt accaccgatg gtaaaacctt gttaggtgcc gatgacaaag caggtattgc
     2821 agaaatcatg accgcgctgg cggtattgca acagaaaaac attccgcatg gtgatattcg
     2881 cgtcgccttt accccggatg aagaagtggg caaaggggcg aaacattttg atgttgatgc
     2941 cttcgatgcc cgctgggctt acactgttga tggtggtggc gtaggcgaac tggagtttga
     3001 aaacttcaac gccgcatcgg tcaatatcaa aattgtcggt aacaatgttc atccgggcac
     3061 ggcgaaagga gtgatggtaa atgcgctgtc gctggcggca cgtattcatg cggaagttcc
     3121 ggcggatgaa agcccggaaa tgacagaagg ctatgaaggt ttctatcacc tggcgagcat
     3181 gaaaggcacc gttgaacggg ccgatatgca ctacatcatc cgtgatttcg accgtaaaca
     3241 gtttgaagcg cgtaaacgta aaatgatgga gatcgccaaa aaagtgggca aagggttaca
     3301 tcctgattgc tacattgaat tggtgattga agacagttac tacaatatgc gcgagaaagt
     3361 ggttgagcat ccgcatattc tcgatatcgc ccagcaggcg atgcgtgact gcgatattga
     3421 accggaactg aaaccgatcc gcggcggtac cgacggcgcg cagttgtcgt ttatgggatt
     3481 accgtgcccg aacctgttca ctggcggtta caactatcat ggtaagcatg agtttgtgac
     3541 tctggaaggt atggaaaaag cggtgcaggt gatcgtccgt attgccgagt taacggcgca
     3601 acggaagtaa gcgaaaaggg atgcggcatg tgatgccgca tccggcttaa atccaaactt
     3661 acccttcgaa gaaccaatac ccgctattga ccagcgccgc gagcatcgcg aggaatgacg
     3721 gatcttccag cgcatcgcca aaattctccg cagtcagcgc aatgttgctg gcgagtgcat
     3781 ccagtgccgg acggtgcggg gaatcgatct tctcaccatt ggtatacacg tcgtcgccaa
     3841 tgcgcaatac gcgcagacca cccaggcgca ccagcacatc accttgtttc agcgcatcgt
     3901 agatttcatc tggctgataa ggcggctccg gcggcgcgat atccagttca tgacgtgact
     3961 gggatataaa ctcgccaaac cattgcttaa agtgttccgg ctggttgatc aattcgagca
     4021 tcatctcacg cagtttatcc atctcttgcg gcagaacgtc cgcaggatga gcgcgaggtg
     4081 gaacatcagg atcgctgtag tagttgccgc ccagttcgcg ttgcagcaca taatcggcaa
     4141 atccgctaat taattcccgc gtatttggcg cgcgaaaacc cacggaatag ttcatcgcat
     4201 tttccagcgc gtagccttca tgcgggaatc ctggcggaat ataaagaata tcgccaggct
     4261 ccagctcttc atcgatgatg gcttcgaacg gatcgacctg taacagatcc ggatgtgggc
     4321 agtgctgttt catttgcagc ttctcgccca ctcgccagcg acgacgtccg gtaccctgaa
     4381 tgataaacac gtcgtactga tcgagatgcg ggccgacgcc gccgccaggt acagaaaaag
     4441 agatcatcag atcatcaata cgccagtccg gtagttcacg gaacggtcgc atcagcgcgg
     4501 cggtcggctc atgccagtgg ttcactgctt gcactaacaa tgaccagtta gtttcaccga
     4561 gatgatcgta gctttcgaac ggaccgtggc tgacctgcca ttttccatcc tggtgactga
     4621 ccagtcgact gtcgacttcg ctttccatcg ccagacccgc caactcgtct ggagagatcg
     4681 ggtcaataaa attattaaag ccgcgtttta acaccaccgg gcgtttctgc cagtgacgtt
     4741 caagaaaatc gggccagtta agagtgagtt ggtattccat gttaagcatc cacaggctgg
     4801 tatctgcaac cgattataac ggatgcttaa cgtaatgcgt gaagtatgga catattttat
     4861 caattagtgt tttttgccgt ttcataatgt ttcagattgt tcattatatt tttgattata
     4921 aacagatttg tgattttcct tgttccagct tgtttcacgt tgtagccctt tgtgcaacga
     4981 tcggtgtagt taatggtgta gttaattgtg atgaccggaa tcaaaattat gagcagagca
     5041 cttaacaaac tgagcgatac acagctgagg aaaatcaacg gcacacccgc ccaaaaaaca
     5101 gcctttctta atgacggtgg aaacctgagc gtcaggcatt caaccagtgg ccttttaacc
     5161 tggtatttca cttacagggc cggaacggga aggggggcac caccggaacg cattaagctg
     5221 ggaaattatc ctgatctgag cctgaaatca gccagggaaa aagccgccca gtgtcgcgca
     5281 tggctggcag aggggaaaaa tccacgtcat gagcttaatt acaccgtaca ggaagcgtta
     5341 aagccggtaa cggttggcga tgcgctcacc tactggcttg agtcgtacgc aaaggaaaac
     5401 cgcgtggatt atgccgccct gaaaaagcgc cttaataatc acgtaataca gcacattggt
     5461 gctatgccgc tggataaatg cgagctacgg cactggctgg cctgttttga ccaggtggca
     5521 aagcgaacgc ctgttactgc cggattcttg ctacagacgt gcaaacaggc gcttaagttc
     5581 tgccggaggc ggcgctatgc aatcagcaac gttcttgatg atatgagtgt ggcggacgtt
     5641 gggaaaaaac cggatataag cgagcgtgtc ttaagcacca aagaactggg cgaattattg
     5701 caggcactgg acaaaaaaat attctccccc tactacatcg cgttaatccg cctcctgatt
     5761 gtgttcggat gccggacggt agaactgagg ttatcggaga tcagcgagtg ggattttacc
     5821 gaaatgctct ggaccgttcc gaaggagcac agcaaaacga aggtagccat attccggcct
     5881 ataccggaag caatactgcc gttcatcacg cagctggtgg agcagaacag gcacacgggc
     5941 ttattgctgg gggaagtgaa acaggaggcc agcgtatcgc agtatggaag attagcgcac
     6001 aggaggctta atcaccctca ctggtcactg catgacatcc ggcgcacctt tacaaccatg
     6061 ctgaacgatt taggcgttga tccgcatgtc gtggagcagc ttacaggcca ccagatgcca
     6121 ggaatgcagc gagtttataa tcattcccgt tatctggatg cgaaacgcaa tgcgctggat
     6181 atgtggacgg agcggttagg gatactggcg ggaacacatg aaaacgtaac cacgctacca
     6241 gtagccagaa gaaaataatt tttttcgtgt tttttcagta tgcgcatact ggatatgcga
     6301 acagatacaa cgtgcaacaa tgagcaacaa tgtggaacaa ctacgaacaa gaggcgaaaa
     6361 agcgtacgga tttataagta gctgattttt aatgtgttac tggtttttta tacactagcg
     6421 aatcactctt taaagagcga gaaaaaaggc atggaacaat attttatgcc tttgtattaa
     6481 catgaattta aaagatttta ttcttacttt ttcattttct gtaaatcata catcctcagt
     6541 atttgccatt catcactcta gcgggaacaa tacgacacaa taagacacct gatgactctt
     6601 taagaaacga aagggggcaa tagtgttaag cactgatcgg tttatacgtg aaaaagaatg
     6661 cgaaaaacta accggcctta gccgtacgtg tcgctaccgc ctggaaaagg ccggacaatt
     6721 cccatcacgt cgtaaacttg gcggtcgttc cgttggctgg tctttatccg aggttctggc
     6781 ctggaaggat agctgcaagg cagttcatta atcacgctgg cggcacacag ccgccacaca
     6841 tcaatcatct gaacacagag ctataaccat gaagattgaa tatacgccag aacgtgggcg
     6901 gggattcgtt cgccctggtg agactggaaa accacaaaat tggggttttt caggcataaa
     6961 aaaagcggcc ccgaaatgga gccgcctttc tgaacagata acccgctgcg ccttatgtgt
     7021 atatgatccc aaacataagc acggggatga tagccgctat caggctggtg ggcaatgcta
     7081 tcagtctggt tcagttcgtt gccatacctg caatgggcgc gtttccctgt actctttaag
     7141 gaattgctca agggcaaaag cacatggcgc gaatctttct gattcatgct ctatctttct
     7201 gcgccgtctt ttccgtgccg gtgataatgt tttggtcaat tctttatcgg tcattgtgtt
     7261 gtcctgcata gcaatgcgcc gtaatacctc acaccacggc gctgatagtt tttatccttt
     7321 gggttctatg ccgcgttgct gtaattcttt gcggagaacg cgtttaatcc aagtggctaa
     7381 agatgtgtcg ccatcggcct tagcagcttc ttctagctgc gctctgaagt cttccggtaa
     7441 tcgcatttgg tatggaggcg aacgtttttc taatggtgtt gacatggtaa ttacccttta
     7501 ttgctatacc tacatggtaa ttaccattgt acttgccatt acacaaaaat caacgccccg
     7561 cagtgctggg aacacatacg gggcgtctaa ccaacaacgt aaactaggag ccgttatggt
     7621 tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt ggcgttttct
     7681 ggcactgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg aacgcgaagc
     7741 gcgtaaccgt tgcccgtccg gttgtgttgc tgtatttgcc gcccgtattc gtcaggaggt
     7801 gcgccatgat taatctgtca cttactgacc ttaaccgcat tcagtttcgt gagaaattca
     7861 cagggcagct actggtcaat gtggataaag gtcgcgtggt gtgtaattac cacttaccag
     7921 atgaagcaat tgtcgccaca agggaatcat tacaggaaat cacaaaacgt gccggaatga
     7981 ttaacacgaa tattcagggg atgctttatg cacaataaaa ccacaccgga cgcagccgcc
     8041 gaagccatca aaacgctgat gcacgcgctg attgatattt ctgttatcgc ggacagggca
     8101 cataagcacg ccaccagcga aacagaatat gccggtgctt tcgttcctca ctcactggct
     8161 gtcatgcaat ttagtgctga tacggcgctg aatgaggcca aagctatcct gattgctgat
     8221 tgtgaaaatg ggggggttat gcgtgatgat cgttttaatt ccctgaaaca ggaattttcc
     8281 ggcgttcctg atgatgcggc tgatgcgctt tcgtcaatgc cagaacttat tagagcggct
     8341 tttttcttac tttccacgag agaatataaa tcaacggggc ttgatgtact gaatatcgcc
     8401 gccgattatg cggaatatgt ggcagaggcg cgttatagaa gaaaatttcc tgaggatgta
     8461 agccatgcgt gatatttacc accaaacaat agaccgcgca tttcttgcac tttctcacag
     8521 tgaaaacatg atggaaatat tgcgcatatg gcttgaaaca cttggcgaca atgaacgcga
     8581 caaacaaaaa tcaagaattg ccacggcatt aataacgctt cttgagcctg taataatgga
     8641 actgcaagaa atagatctat tgcacgacag atataaagaa cagcacaccg gagaataaaa
     8701 ataatgaaac ttaaatattc tggcttaact gccagtggca acactcaccc taaatttacg
     8761 cgcggtgata tttaccgcga ccagtacggc ggcacggtaa tgattaaggg cgtggaagaa
     8821 cggcgcgtaa cctaccgccg tgaaggttac gaatatgatt gcgtgatgcc tgtttatcag
     8881 ttccggcgtg atttttctct ggtacagacc gcgccgcata acgtgcccac cagcaacgcc
     8941 agggcacggg caaacatcca gaagctgaaa accatggtta acggattcag gggcaagaaa
     9001 tgaaaagcgc accgaactta aaaaaacagc cttacgacaa gatgaccgaa gtcattattt
     9061 ttgcgggtag tgatgcctgg gcacatgcga aacagtggca ggaacaggac gggcgactgg
     9121 ctggcgataa tgtgcctccc gttgtgctgg ctgatgatca actggatgaa ctggcagaac
     9181 tgagaatcat cgacgagggg cgctattgtg tccggctgta caaggcaggc cacatcaggc
     9241 catcaaatat taatgccatt gcgcacaagc tggcggcggc gggtgtaact gatgcgaatt
     9301 attaccccga agggatgcac agccatatgc gggagaactg gcgcgaatac ctggaacggg
     9361 tgcgcgggaa agagccggcg gaagaaaaaa accaccagcg aaaaaccacg ctaccgatga
     9421 gcgttggatc taccggatac gacacgcaac tggattacgt ggttaagggg attattccgg
     9481 cggtatcgct atgcagcata tacggggcta gcgggtccta taaatcattc cttgccggat
     9541 cgtgggcgtg ccatgttgcc actggtcgcc agtggggagg ccgcagggtt gcacatggtg
     9601 cggttctcta tgtggttggt gaaggcggta taggtgttcc gcgtcgtgta aaagcctggg
     9661 aggttgtgca cgatgagcag gtgaaaaatc tgtatctggt aaaccgcccc atctttccgg
     9721 ctgccccgct tgatgttgat gaaatggtta tcgctgcccg tcaggtggag cgggaaacgg
     9781 gtaaacctgt acgcatgatt attctggata cgctggcgcg ttgctttggt gggaatgatg
     9841 aaaatgattc ccgtgatatg ggggcgttta tccgtggttg tgacgaactg aaacgacgca
     9901 caggggccac ggtgctggtg gttcaccatt ccggcaagga tgagacgaaa ggcgcgcgcg
     9961 gttccagtgc atttcgtgct tcgctggatg ctgaataccg gatacgcagg gaggacgcag
    10021 gaagcgaagc gctggttatc tcatgcacca aaatgaagga cgcggaggaa ctcaaagaag
    10081 ccgcatatga cttacgcgtg gtggagcttt ttaccgacgc tgacggtgaa ttaatcacgt
    10141 cgctggtggt ggtggatgat ccgcgccctc ctgttgaact ggagcgcatc gaggaggcag
    10201 ggaacaagac ggaaaaccat accgcgctat gggggtgcat ccgttcacgc acacagaacg
    10261 gcgacaagtg cacgatcccg ctgttacgtg atgacatgaa aaagctgggg tatgaaatga
    10321 aaaacttccg gcgctggctg tacaagctgg aaaaagatgg ggttattcgt atcgatgggg
    10381 atgatgtagc gccgctataa aagtgaggag caaaagcgag ggggatagaa agagggccaa
    10441 aattagcccg ctctccctca cttttcgacc tgtatacatc ctcaaaagtg aggggtaaaa
    10501 aaatacttat gaaacacaca catagaaaaa ccgaaaatcc caactgcgac gaagtgagac
    10561 gcttgaaaaa gtgaggcgaa aaagtgagag gttgcgagaa atgacccaaa aacgcagaga
    10621 cagaacagag ccaaaatata aagcgttaga catgactgag cacaccttaa aggtggcaat
    10681 cagaacgata gaccgccaca cgcgggaagg atacgcgaag gaacatcccg acctgataag
    10741 cgcattcatg accacggcgg cggcaaactt tgccacgctg acagaacggg agattgccga
    10801 agcggaacag gtaacaacca tcaacgttaa aaccggagag gtggaattat gacggcacag
    10861 atagccgctt acgggcggct ggtggacgac ccgcaggtaa aacagaccag caagggcaca
    10921 ccgatgacgc tggcgcgtat ggcggtatct ttgccatgca gccaggcaca ggacggacag
    10981 gcgacgttat ggttatcggt catcgcattt ggcaaacagg ccggcttcct ggctaaacat
    11041 caaaaaggcg atgttgccag cgtatccggc acgatgcagg taagccagtg gaccggacag
    11101 aacggagaaa cgcggcaggg ctggcaggtt atcgcagaca gcgtaatcag tgcccgcgcg
    11161 gcacgtccag gcgggaacag acgcaaaacc acaggcacac agggtaatca gccaccagcg
    11221 ggaggcgatg acccttacgg tgatgatatt tcgttctgag ggggtgacga tggtacatga
    11281 ccgcatagcg gaggaactgg aggcgaaagg cttttaccgg agggcggcgg cgcgatgggg
    11341 tgaagtcatg cagctggtgg agacagacaa ggaacggcat cacatcacga tgcgacggct
    11401 ggaatgttca aggaaggcac agagggcacc ggagccgccg gataatttcg gagacctgaa
    11461 aaaggcagtc gatcgcactt atgccgaaat gggtatagat ggtgctggtg atgaaatatg
    11521 gcgcaattac caggacagct aatcaaacag ccggagaaat ccggcttttt ttgcaccagt
    11581 tgaaacggta tggcgcatta ccgggttttc gtcacggtca ggcatagtta ctatctgaaa
    11641 caaacagaca caacagagga aaaaaacaat gccgatgaaa tttgatgaga tattaaaaca
    11701 gcgtgataaa taccatgctg acaacatgga gacgatgagc atcaatgatt accgcgcatt
    11761 cctggagacg ggcgcactga ttgaaaagga tcagcatggt tttgtgagat gtgctctatc
    11821 cggtgaaatg ctggcggtaa atcctgaaca gatagatgca ttgatagaat ttctgaaaga
    11881 gatcagagac tgagccagca cacagcacac atagccggag caatccggct tttttgcgcc
    11941 caaaaaaagc ccgataaggt cagagggttc ttatcgggct tttgcatatg aggtttttgg
    12001 atgcactggc gttcgtgatc gggataatca tttcataatt tgcaacataa ctcaatatca
    12061 ttgcataaaa tgcaattctg attataatca ggactggata aacatccagt tatgattttt
    12121 ttagtcgaag aggaatttct tactatggct gaagagaaaa aaagcggtgt ttcggtgtac
    12181 ataagccccg aaatcgtgga ggtgctcaag cagcgccaca aaaaaaacta tgaagctggc
    12241 gttgcggctg gactggatcc gctgatgacg ccggagccgt cgataggttc acttgtacgc
    12301 tcttatttac ttgcggcgct tgggatgcat aaaaattatg ggggtgaata atggcaggca
    12361 aagcaacggc acttaacact aaccagcttt tcatgtacct gaatcgcggg gatattgcgg
    12421 attttaaatt cagccctctg tttaccacgc tgtttttccc gaacgtggcg acattcagca
    12481 cgcaaaacat catgctggat accctggaca ttgaagaagt caccatgtcg gcgttttgtt
    12541 cgcctatggt gggtagccag gttcagcgcg ataaagggta cgaaaccagc acaatcaaac
    12601 ctggctacat gaagccaaag cacgaaatcg atccaacgaa aaccatcatg cgcatggctg
    12661 gagaagatcc ggcacagctt aacgacccta cctatcgccg tatgcgcctg attactggca
    12721 acatgcgccg ccagataaac gccattaagg cacgcgtgga atggctggcg gtgaatgcgg
    12781 taacgaccgg aaaaaacatc attgagggcg aaggcataga acgctatgaa atcgactgga
    12841 aaataccgga aaactgcatc atagagcagg ccaagggtaa aaaatggtcc gagcaggata
    12901 aagacatgca cgacccaatc tatgacatcg agctttatgc tgatcaggct ggttgccccg
    12961 caaacgtcat gattatgggc gctgaggtat ggcgcacatt acgcagcttt aaaaaattcc
    13021 gtgagctgta cgatctttcc cgtggttcag aatccgccgc cgaactggcc tgtaaaaacc
    13081 tgggcgaagt ggtgagcttt aaaggctatc ttggtgatct ggcccttatc gtctattccg
    13141 gcaaatacac tgacagcgat ggtaccgaaa aatatttcct tgagcctgat ttgctggtcc
    13201 tgggtaacac caacaataaa gggctggtgg cctatggtgc gataatggat caggaagcgg
    13261 taagaacggg cgcaacacaa aacatgtttt acccgaaaaa ctggattgag gacggcgatc
    13321 cggcgattga gtacgtgcag acacacagtg caccgcagcc ggtaccggca gatattcgca
    13381 aatttgttac cgtcaaaatt ggttaacggg gggattctat ggacactcca tacattgagt
    13441 tatttgcagg cagtcagcag gtatccacga cgttggtaca ttttgccgct gatgctggcg
    13501 ttattcagga atttaccccg ctgatgctgg cagacaatgg cgagtttaag ccgtgggatg
    13561 gtcaggaatc tggcaaggct gtttatctga cctcgtaccc tgtggacacg tcaaagcaga
    13621 aatcagcaca gtgttacaag acggggatat tcaatattgc cgccgttaac tggcctgaga
    13681 gcgtcgacac cgatgcgaaa aaatgcgccg cctttgcggg ttccggcgta tccgttcagc
    13741 cgctggcccg ataagcaggg ggaacgatgg caacgaatga aagcatcatg acgctaccgc
    13801 tggcgagtaa atttaaagtc gaagcgcggg caatggctga cagaggttta tcaacctatg
    13861 aggccgtata tcaactaaac aaactggaag agcaggacaa gccgtgcgct gatgcgatta
    13921 tggcgcttca tgagcataaa gactatcagc cgctgttgcg tgcaatggca aacgtgcctt
    13981 gtatcgatgt tggtacggct aaaagcatcc ttagcatgac catagagcag gaacgtccga
    14041 aggttgcgcc ggaacttacc gcagcctttg aaaactttat ggacatgcac agcccgcaag
    14101 ccgtatcagc tggcatggca tacgatggca gaaaccaggg cgatgacggc gacatcgatc
    14161 gcatactgaa aaccatctga gacaaggccg gagaaatccg gcttttttac gggtccttcc
    14221 tggaattatg gcccgttacg gggcggcgac ctcgcggatt ttcgctattt atgagccttt
    14281 tcagggtggt ggtggtggtt ttgttgtttg atctatcttt atgaatgaaa agggaaagat
    14341 gcaagcaata caccaacctg aagcagtaat taagttggtg tattaatgaa atctcatctg
    14401 atgaacaaaa aaaacatggc gaaaagctgc cgtgtaagtg cgacagcgtt cgacaagtgg
    14461 ggagtaactc ccgttgagcg taaaggccgt gaggcgtttt atgatgttgc cagcgtgata
    14521 gaaaataggg ttaacaatgc aattagccag cttataaacg acaaaggcga gattgacgat
    14581 gatgaacttt tacgagtcag gatcagatta ctgacagcac aggcagaggc gcaggaactt
    14641 aaaaacgatc gcgatcgcgg tgacgtgatt gatactgagt tttgcctgta cgcgctttca
    14701 aagctggcga gtcagatttc atcaatcatg gacagcctcc cgcttactat gcaaaggagc
    14761 ttcccacaga ttacccccgc catgctggac aggcttaaaa gggaagtggt taaagcctgt
    14821 aatgccagtg ccagagttgc cgacaacctc ccacagatac tggctgatta cttgaaagaa
    14881 acaaccggaa acgtaccgga aaagttgcta cagaagaaag gcgagtaaca gacgcgcaat
    14941 tattgaacaa aaatgagaaa ggatctgata atggcatttt ctggctaaaa tgcctcgtgt
    15001 cagatccttt ctttttttaa tattttcatt gttaaacagt ctgttacaga gaagaagatc
    15061 ggatctgact tttgccggaa aattctcata aacagataaa aaccgcgagg tcgccgcccc
    15121 gtaacgggcc ataattccag gaaggacccg acgacaccag actatcagag cgatgggggc
    15181 acaatgacag aagccgaact actgggatta atccgccgcg tcgccggaat cagccagcag
    15241 catgacgaac aggccacaca gccggacagc gtgaccgctg aaaattatgt gcgtgttgta
    15301 gcggaagtga tgcgccgtga tggtatagaa cttaatggcg ttgatatgcg caacatacgg
    15361 aaccgcgttc ttgaaatgct ggcctacaat cgccgcgttg aactgtatcg ggagaaagaa
    15421 aaaataacgt accactggaa gaagccggaa cggctgcggc ggtaacttgc tgatattccc
    15481 gataacgcaa aattgcgctg gctacatcct cacatctgag gactgttaat cagaaggccg
    15541 gaatatattc acaaactacg aaattttcgt agtttaatta atggtcagat taatcgctaa
    15601 cccgctgata ttttcggaaa cctcaatttg aggaagtcgg aagcgtcacc cttcatattc
    15661 tgaatggtca taatgaccac gcagaccatc aaggcaacca caacgaacat cgttacggtt
    15721 ggagctttta ccccactggg ggaaaggtat tacgataatc ataacaccta ccgaacaggt
    15781 aaaacccacc agcctgatta acagttaacc ggaaaaaaaa tccggtagcc ccgttatggg
    15841 gatccccata tcgacattaa cgcccctcat gaattggtgc gcttccccct ggaaagatta
    15901 cccgcctgta tatttcttgt gtctatttgt tccacgttgt ttcatacagt gcaccgaacg
    15961 gtgtagttaa tggtgtagtc aatttgctgt ttttagcact ttttgcagtg tgggttgttt
    16021 aattaaatca atgggttgcc ttgctgtggt gataaaagga catatttatt catctttcgg
    16081 cgtagaatgc tggcgaccaa aaatcacctc catccgcgca ccgccaagca tgctctctcc
    16141 ggcgacgatt ttaccctcat attgcttggt gatttcgcgg gctaccgcca gccccacacc
    16201 ttgcccaggg cgtaaagtat caacccgttg accacggtcg aaaatgacct ctcgcttgct
    16261 taatggaata ccggggccat catcctcgac cacaatatag agatgctcgt cggtttgcct
    16321 tgcagaaatt tcgacaaact cgaggcaata tttacaggca ttatccagta cattgcccat
    16381 cacctcgaca aaatcgttct gctcaccgac aaagctgatc tctggcgaaa tatcgagaga
    16441 gatattgacc cctttgcgtt gatacacttt gttcagcgcc gaggtgagat tgtccagcag
    16501 tggggcgacc ggatgcagct cgcggctgag caatgtcccg ccgtgcatac tggcacgatg
    16561 caggtagtag ccaatttgct gtgaaatgcg gctgatttgc tccagcatta ccggttcagc
    16621 atcactgacg ctcatctttt cactacgtag agaacgcagc gtactttgca gcaccgccag
    16681 tggcgttttc agactatggg tcaggtcggt gagcgttgta cgatatttgt cgtaacgttc
    16741 gcgttcactt tttaacaatc ggttcaggtt tcgtaccaga ctggtcagtt ctcgcgttgt
    16801 ggctggattg agcaattcgc ggttatgttc ttccagttcg cggacttctt ttgccagggc
    16861 ttcgatgggg cgtaaactcc accaggcggc gacccacagc agcgggatca ctaacagcag
    16921 attggctgag agcacataga taaaccagct ccagaccata taggaacttt ttagctccac
    16981 cggaatggta tccaccacca caatggttaa ctttggcatc cgcgatgttg ccgggtagac
    17041 gtttaccgcc accgagtggg tcatctccgc gtcgtcatca tcttcccgca cttcctgcaa
    17101 ctgttgctgt atcgaatgat ctccactcag caagaggctg gtatcgttaa catccgcttc
    17161 aatttcatga aaaccattcg atttcagcca gtcaggctgg atcatcttca tcagccaggg
    17221 cacgtcacgt tgcgcccata aaagctgccc gttctcatca taaattagcg tcatggtggg
    17281 gctttgcttg tcgatatttt cgggtaactc gacatgcaac ttattgtttt cccacttcgc
    17341 aagggtatag aacagattgc tctcgccacg taacagccga aacgtagttt tatcgaaact
    17401 gacgctataa ccgatcagcg cgaccattcc gtaggcaagc gaaagcacca gtactaccgc
    17461 tgccgttgcc aacagaaaac gtacccgcag cgagagcggg aaaaaaagac gcagtaattt
    17521 tttcatcagc gcaattcgaa caaatagccc tggccgcgaa cggtagtaat cacttcttgc
    17581 ggatattgtg cctgaatttt tttgcgcaga cgtcccatca gtacatcaat ggtatggctt
    17641 tcccgcagct ccgcatccgg atagagttgt agcattaacg aatctttgct gaccactttg
    17701 ccattattgc gtatcaacgt ttccataatg gtgtattcga acgcggtcag tttgatcact
    17761 tcgtcattaa tagataattc acggcgagag agatcaacct gaaacggggg gagcgaaatg
    17821 acctgtgaag ccaggccgct attacgccgc attaatgcct gcattcgcgc catcacctct
    17881 tcaatatgaa acggtttagt cacgtaatca tcagcaccgg cacttaatac ttcgactttg
    17941 tcctgccagc tttcacgggc ggttaatacc agaatcggca gtgaaacatc attgctacgc
    18001 cagcggcaaa tcagtgacag accgtcctcg tctggcaatc cgagatcgac aatcgcaata
    18061 tccggtaaat gttcattgag ataataatcg gcttctttgg catcttctgc atcatcgacc
    18121 tgatgaccag catcctgaat ctgaacttta aggtggtgac gtaacaacgc attgtcttca
    18181 acaaccagta cgcgcatttt tatttctccc tgtcttaatt attaaaatag tgtaacgcga
    18241 ttatgtggtt atgggggtaa acattaaata aaccagcggg ggaggggagg taaagtgaaa
    18301 aaataaaaag cggataatct taataagcag gccggacagc atcgccatcc ggcactggta
    18361 cgagtttatt tcagctcatc aaccatggtg atggcgcgac caatgtagtt agccggcgtc
    18421 atcgctttca ggcgggcttt ctcttcttct ggcaacgcca gaccgtcgat aaactgcttc
    18481 atgccttcgg cgtcaacgcg cttaccgcga gtcagctctt tcagcttctc gtacggtttt
    18541 tcgatgccat agcgacgcat aactgtctgg attggttctg ccagcacttc ccagttgtga
    18601 tccagctcat ccagcagatg gtcacggttc acttccagtt tgctcacgcc tttcagggtg
    18661 gattgatacg caatcagcgc ataaccgata cccacgccga ggttacgcag cacggtggag
    18721 tcggtcaggt cacgctgcca gcgggaaacc ggcagtttgc ttgccagatg ctgcaatacc
    18781 gcgttggaaa ggcccagatt cccttcggag ttttcgaagt cgatcgggtt aactttatgc
    18841 ggcatggtgg aagaaccaat ctcaccagca atggttttct gtttgaagtg gttaagggcg
    18901 atataacccc agacgtcacg gtcaaagtcg atcagaatgg tgttgaagcg cgcaacgcaa
    18961 tcaaacagtt cggcaatgta gtcgtgcggt tcaatctggg tagtgtacgg gttccattga
    19021 atacccagcg aggtgacgaa ctcttcgctg aactgatgcc agtcaacttc cgggtaagcg
    19081 gcgatgtggg cgttatagtt accgaccgcg ccgttgattt tgccgaggat ctccacctgg
    19141 ttaagctggc ggtactggcg ctccatacgg taggcgacgt ttgccatctc tttaccgatg
    19201 gttgacggcg tggctggctg accgtgggta cgggacagca gcgggatatc gcgatactga
    19261 acggcgagat ctttaatgcc atcaattagt tgacgccagt acggcaggat cacttcatca
    19321 cgcgcggttt tcagcattaa tgcgtgggag aggttattga tatcttccga agtacaggca
    19381 aagtggatga attcagaaac cgcgtgcagt tccgggatct ccgccacttt ttctttcagg
    19441 aaatactcaa ccgctttaac gtcgtggtta gtagtacgct cgatggtttt gatgcgtgcg
    19501 gcatcttctt cgctgaaatt ggcgacaatt gcatcaaggt aaccgattgc gtcggcagca
    19561 aaagcaggaa cttccttgat cgctgcgtgc gcggccagtt tttgcagcca acgtacttca
    19621 acttgtacac ggaatttcag caaaccatat tcgctgaaaa tcccgcgcag cgcgctgact
    19681 ttatcgccgt agcgtccatc gacaggggaa acggcggtca gtgaggataa ttccatagat
    19741 cacaactccg gggttaaatg agcaagaatt tgttttgcct gagtggtcag gcgattacga
    19801 gaaaacatca gttgcagacg tccgccgccg acctggtgcc agagcacggc ggcgcgaatg
    19861 cctgccagca gggttgcgcg aactttcgcc tgcacttgtg ggctttgcag tacagcaggg
    19921 gaaccggtga cctgaatgcg cgggccaagc gggctgatca catcaacata gatagcagcc
    19981 atcgcgctca tcagcgtttc ggactgtaaa tcgaagtgtt cgagctggcg ttgcaggccg
    20041 ttgatccggt tgcccagagt gtcgagcgca cctttcgctg aggagagttt gcgcttaagc
    20101 accatcaggc tgagtgtgta gcgggttaat tcggcgttta agccctggcg actgctggca
    20161 ttgagcacgc cgagcagggt ttccagcccg acgcgcaggt tggcttcgct accgccaaaa
    20221 accgccagcg tcgagctggg gttcatatca ataatactgt tgagtgagac gtgtagcgca
    20281 tcggcatcac aatgcccctg gtgagcgagt tgttgcacca ggcgtgccga ctgacaaata
    20341 ccggccaggg cgagggtgat gtcatagtaa ttctttgcca cgttcactgc ttccttgttt
    20401 taagtaaaga taataatcag atcggcagcg gcagacgctg ctcaataata ccgccaccga
    20461 ggcacacttc accgttatag aagacggcag actggcccgg cgtcacggcg gcaacgggct
    20521 catcgaaaat cacttcaatg cgatcatcgt ccagcgcctt gacggtgcag gggatatcgg
    20581 tctggcgata gcgggttttt accgtgcaac gcatagtacc ggtgaatggt tcacgatcga
    20641 cccagtgcag ctgctgggca atcagaccga cagacatcag acgcgggtgt tcatggccct
    20701 gcgcaacaat cagaatgttg ttttcgacgt ctttatcgac cacataccac ggttcttcgg
    20761 tgccttcttt ggtgccaccg atacccagac ctttgcgctg accgagggtg tgatacatca
    20821 gcccctgatg ctcaccaatt tcatcgccat cgactgtaat gattttgccc ggttgcgccg
    20881 ggagataacg gccaaggaac tcgcggaatt tacgttcgcc aatgaagcaa atgccggtag
    20941 agtctttttt cttcgcggtg accagaccaa gatcttcagc aatcttacgc acctgcggtt
    21001 tttccagttc gccgaccggg aacaggcttt gcgcaatctg ctcatggctg agcgtataaa
    21061 ggaagtagct ctggtcttta ttgctgtcca gaccacgcag caggcggctc ttgccatcga
    21121 catctgcccg acgaacgtaa tgaccggtag cgatataatc ggcacctaaa tcttcggcgg
    21181 caaattcgag gaaggcttta aatttgatct ctttgttgca cagaatatcc ggattcggcg
    21241 tgcgaccggc tttatattcg gcaaggaaca gttcgaagac gttgtcccag tactcggcag
    21301 caaagttaac ggtgtgcagt tcaatgccga gcttgtcgca gacagcctgg gcatcagcca
    21361 gatccgctgc cgctgtgcaa tattcctcac cgtcgtcttc ttcccagttc ttcataaaca
    21421 ggccttcgac ctgatatccc tgttgttgca acagccaggc agaaacggag gaatcgacac
    21481 cgccggacat gccgacgatt actttttttg cggtttcaga cattggatca ctcacgacat
    21541 tgaacttcaa ggcggcgtat tctatcacgc agccttttag atgacaccct ttgtaaaagg
    21601 ccagttaaaa tcgccaatca tctccagcgg atagcgttgc ccgctttgat aacaacggat
    21661 actttccgcc accagcggcg aacgaagatt tgacgcctgt aaaatttctt cggcgctgac
    21721 ccaacgacag cagtcgatat cgctgtcatg aggctgggtc gggcatattt gctcaagctc
    21781 aatggcaaag aggaaacgca aaaacggcgt tttatctggc gcaatccact gatgcatacg
    21841 aataaagtgt tgcggctgcg cgctgatgcc ggtttcttcc cacagctcac gggcggctgc
    21901 ttccactaag gtttcatcgg cttccagatg cccggcaggt tggttccata aggctttacc
    21961 attaatcgtc tcttcaacga ctaaaaattt cccctctgcg tgcactacgc aagcaacggt
    22021 aacgtgcggt ttaaacattg tgcatcctta ttctgttgct tctcgccatt cgccattggc
    22081 aaggttatcc aaagagtaat cacccatcgc atagcgaatc agtcgcagcg tggggaaacc
    22141 aacatgggcg gtcatgcggc gcacctggcg attacgtcct tcatataagg tgatcttcag
    22201 ccagctggtg ggaatacttt tgcgttcacg aattggtgga ttccgcggcc ataaccacgc
    22261 gggttcgtca accagctccg cgcctgcggg cagggtaggg ccatcattta aggttacgcc
    22321 attgcgcaag gcttcaagtg cgtcttgtgt gggaatacct tccacctgca cataatagat
    22381 ttttccggtg cgtttgcccg gctgggttaa acgcgcctgc aacgcgccgt tattggtcag
    22441 caccagtaac ccttcgctat cgcggtcaag acgacctgct gcataaacgc cctgaaccgg
    22501 gatgaattct tttaatgttt tgcgtccggc ttcatcggtg aactgcggaa gaacatcgta
    22561 gggtttattg aacaggatca cacgcgtggg ctggttttca ggtttacgcc tggtagaacg
    22621 ttgtgagctg aatcgcttaa cctggtgatt tctaaaagaa gttttttgca tggtattttc
    22681 agagattatg aattgccgca ttatagccta ataacgcgca tctttcatga cggcaaacaa
    22741 tagggtagta ttgacaagcc aattacaaat cattaacaaa aaattgctct aaagcatccg
    22801 tatcgcagga cgcaaacgca tatgcaacgt ggtggcagac gagcaaacca gtagcgctcg
    22861 aaggagaggt gaatggaaag taaagtagtt gttccggcac aaggcaagaa gatcaccctg
    22921 caaaacggca aactcaacgt tcctgaaaat ccgattatcc cttacattga aggtgatgga
    22981 atcggtgtag atgtaacccc agccatgctg aaagtggtcg acgctgcagt cgagaaagcc
    23041 tataaaggcg agcgtaaaat ctcctggatg gaaatttaca ccggtgaaaa atccacacag
    23101 gtttatggtc aggacgtctg gctgcctgct gaaacccttg atctgattcg tgaatatcgc
    23161 gttgccatta aaggcccgct gaccactccg gttggtggcg gtattcgctc tctgaacgtt
    23221 gccctgcgcc aggaactgga tctctacatc tgcctgcgtc cggtacgtta ctatcagggc
    23281 actccaagcc cggttaaaca ccctgaactg accgatatgg ttatcttccg tgaaaactcg
    23341 gaagacattt atgcgggtat cgaatggaaa gctgactctg ccgacgccga gaaagtgatt
    23401 aaattcctgc gtgaagagat gggcgtgaag aaaattcgct tcccggaaca ttgcggtatc
    23461 ggtattaagc cgtgttctga agaaggcacc aaacgtctgg ttcgtgcagc gatcgaatac
    23521 gcaattgcta acgatcgtga ctctgtgact ctggtgcaca aaggcaacat catgaagttc
    23581 accgaaggcg cgtttaaaga ctggggctac gagctggcgc gtgaagagtt tggcggtgaa
    23641 ctgatcgacg gcggcccgtg gctgaaagtt aaaaacccga acaccggcaa agagatcgtc
    23701 attaaagacg tgattgctga tgcattcctg caacagatcc tgctgcgtcc ggctgaatat
    23761 gatgttatcg cctgtatgaa cctgaacggt gactacattt ctgacgccct ggcagcgcag
    23821 gttggcggta tcggtatcgc ccctggcgca aacatcggtg acgaatgcgc cctgtttgaa
    23881 gccacccacg gtactgcgcc gaaatatgcc ggtcaggaca aagttaaccc aggttctatt
    23941 attctctctg ctgagatgat gttacgccat atgggttgga ctgaagcggc tgacctgatt
    24001 gttaaaggta tggaaggcgc aatcaatgcc aagaccgtaa cctatgactt cgaacgtctg
    24061 atggaaggcg ctaaactgct gaaatgttca gagtttggtg acgcgatcat caagaatatg
    24121 taatcactac atgtgttaaa tattgaaacg ggcgtataac acgcccgttg ttttatgatg
    24181 atttaaaatc ttccccaaaa ctttccccaa aacccttccc caaaactggc tattttctat
    24241 gctgttttga tatctacgat aatccagtct ttaccacgat catcattgta tcggtcggtc
    24301 atttttctgg atttatgacc taacaacttt tgcgtatcca gaccctgttc ccgatataac
    24361 cgttctgaca gagatcgctg ctcatgaaat gtgggcgcag ttccttgctc ccattttatg
    24421 ccacattttt ccctggcctt tttaaaagcc gttgtaagag tatttgcaga cacctggtct
    24481 cctctgtttg cttgagaggt agtgtgacgg taatggacca gatatttact aacaacagca
    24541 tccctgcact gagatataac ttcacgaagg gtaatattca gagcatcgca tttcaggtta
    24601 agcggaatag caagttttga accggttttt tcctgagtaa tgtgcaacat gtcgtcccag
    24661 atatcagaga atttcaaatt gcagatatcg cttaaacgtt gtccagtgac aagagcaagt
    24721 agcatcccgc attttaaata gggctgccgt ctgcttacgc tgtcaaatat tgcctgccat
    24781 tcgggcagtg ataatctttg gcggtttact cgatttcgcg gttgttttgt tgcctgcgct
    24841 gggttaaatc ctggcggaac atgtcctgcg tgttgtgctt ctttgaagac gtcgatcaac
    24901 accattctca cgacttgcgc catcctgtta tgaccttcag cctttacagc atcaattatt
    24961 tcggcaatat caagtgcggt aatatccttg aggtgttgca ttccacaatg ctcacggaaa
    25021 agacggatgg gtttgccttt ttgccgatag gagttgggtc ttagttcatt atgttgcagc
    25081 ctgtcctcct ggatagaatt atatttatca agccattctg tcaccgtaat gtctgagcgc
    25141 ctgcctttca ttctttccag acgctcattg acgcttaata tttgtcgggt acgttgttca
    25201 gcaataatgg tatttgcttc agtagcaact tgttttgctt cattctcatc agttcctaag
    25261 ctatgaaaac gaccggatag tggatgtttg tattgccaat atacctttcc ggttcgctta
    25321 tctaatttgc aatataaatt gggtatagag attttgtgag atcgggggct agcagccatc
    25381 agcgattatc cgttggagtt ttgggtttgc gtttattggg agttgcggtt ctgcaagcgt
    25441 tcctacaaaa cgggaatttc ggtcaatcat ccagtagcga ccaactttta tagcgggtgg
    25501 ggccatcatt ttcccttgcg cgtatttttt cagaactcgc tcacttggtg ctaagtcccc
    25561 aaattcttct ttagcccagt cccgtaaagt gattagtcga gacatttgtc ctcctcttag
    25621 ctgctgaggg agtttgtgac cgatatatct gacatgatat taagctcatg gcaggtacat
    25681 ctcttgactg gtcatagaga taaatttaat gctgagaaat gcagtattga atttatcaat
    25741 ttttcaccag cttctggtct ttctggctgt catgcgcaaa acatatggct ttaataaaaa
    25801 actggattgg gttagcaacg agcaactttc cgaattaacc gggatattgc cgcacaagtg
    25861 ttctgctgca aaaagtgttc tggtaaagcg tgggattttt attcagagcg ggcggaatac
    25921 cggcattaat aatgtggtca gtgaatggtc aacattaccc gaatcaggta agaaaaataa
    25981 agtttacctg aaagaggtaa atttacctga atcaggtaaa aaaagtttac ccaaatcagg
    26041 taaaggcgtt tacccgaatc aggtaaacac aaaagacaaa ctaacaaaag acaatataaa
    26101 acctttttcg tccgagaatt ctggcgaatc ctctgaccaa ccagaaaacg atcttcctgt
    26161 ggtgaaaccg gatgctgcaa ttcagagcgg cagcaagtgg gggacagcag aagacctgac
    26221 cgccgcagag tggatgtttg acatggtgaa gaccatcgcg ccatcagcca gaaaaccgaa
    26281 ttttgcaggg tgggctaacg atatccgcct gatgcgtgaa cgtgacggac gtaaccaccg
    26341 cgacatgtgc gtgctgttcc gctgggcatg ccaggacaac ttctggtccg gtaacgtgct
    26401 aagtccggcc aaactccgcg acaagtggac ccagctcgaa atcaaccgta acaagcaaca
    26461 ggcaggcatg atagccagca aaccaaaact cgacctgaca aacactgact ggatttacgg
    26521 ggtggattta tgaaaaacat cgccgcacag atggttaact ttgaccgtga gcagatgcgt
    26581 cggatcgcca acaacatgcc ggaacagtac gacgaaaagc cgcaggtaca gcaggtagcg
    26641 cagatcatca acggtgtgtt cagccagtta ctggcaactt tcccggcgag tctggctaac
    26701 cgtgaccaga acgaactgaa cgaaatccgc cgccagtggg ttctggcttt ccgggaaaac
    26761 gggatcacca caatggaaca ggttaacgca ggaatgcgcg tagcccgtcg gcagaatcga
    26821 ccatttctgc catcacccgg gcagtttgtt gcatggtgcc gggaagaagc atccgttatc
    26881 gccggactgc caaacgtcag cgagctggtt gatatggttt acgagtattg ccggaagcgt
    26941 ggcctgtatc cggatgcaga gtcttatccg tggaaatcga acgcgcatta ctggttggtt
    27001 accaacttgt accagaacat gcgggccaat gcgctgactg acgcggaatt acggcgcaag
    27061 gctgccgatg aactgacctg tatgacagcg cgaattaacc gtggtgagac gatacctgaa
    27121 ccagtaaaac aacttcctgt tatgggcggt agacctctaa atcgtgcaca ggctctggcg
    27181 aagatcgcag aaattaaagc taagttcgga ctgaaaggag caagtgtatg acgggcaaag
    27241 aggcaattat tcattacctg gggacgcata agagcttctg tgcacaggac gttgccgcgg
    27301 taacaggtgc aaccgtaacc agcataaatc aggctgcggc taaaatggcg cgggcaggaa
    27361 tcctggtcgt tgatggtaag gtctggcgaa cggtgtatta ccggttcgct accagagaag
    27421 aacgggaagg aaaggtgagc acgaatctga tttttaagga gtgtcgccag agtgccgcga
    27481 tgaaacgggt attgagggta tataaaagaa catcaatggg tacacaatga tgaaacaggt
    27541 gagttgagtt caaactgtag tacaattctc tccagtttga acaggaaaga atatgttatg
    27601 aacccttata tttatcttgg tggtgcaata cttgcagagg tcattggtac aaccttaatg
    27661 aagttttcag aaggttttac acggttatgg ccatctgttg gtacaattat ttgttattgt
    27721 gcatcattct ggttattagc tcagacgctg gcttatattc ctacagggat tgcttatgct
    27781 atctggtcag gagtcggtat tgtcctgatt agcttactat catgggggtt tttcggccaa
    27841 cggctggacc tgccagccgt tataggcatg atgttgattt gtgccggtgt gttggttatt
    27901 aatttattgt cacgaagcac accacattaa aaataatttc ttttaaaaga ctgcaatatg
    27961 gcggttcata tctttacatg ggccgctttt gttaatgttt ttagtttttg tgtattcttt
    28021 tgtgccttca agattattgc gtaagcaaat tgc
//
LOCUS       DAZKFZ010000044        27787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000044 DAZKFZ010000000
VERSION     DAZKFZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            87..749
                     /gene="acrS"
                     /locus_tag="ACHOQW_001736"
     CDS             87..749
                     /gene="acrS"
                     /locus_tag="ACHOQW_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP9572823.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(752..931)
                     /gene="yhdU"
                     /locus_tag="ACHOQW_001737"
     CDS             complement(752..931)
                     /gene="yhdU"
                     /locus_tag="ACHOQW_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP9572824.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1015..1899)
                     /gene="yhdJ"
                     /locus_tag="ACHOQW_001738"
     CDS             complement(1015..1899)
                     /gene="yhdJ"
                     /locus_tag="ACHOQW_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9572825.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            complement(1984..2280)
                     /gene="fis"
                     /locus_tag="ACHOQW_001739"
     CDS             complement(1984..2280)
                     /gene="fis"
                     /locus_tag="ACHOQW_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP9572826.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2306..3271)
                     /gene="dusB"
                     /locus_tag="ACHOQW_001740"
     CDS             complement(2306..3271)
                     /gene="dusB"
                     /locus_tag="ACHOQW_001740"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9572827.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3600..4481)
                     /gene="prmA"
                     /locus_tag="ACHOQW_001741"
     CDS             complement(3600..4481)
                     /gene="prmA"
                     /locus_tag="ACHOQW_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9572828.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4493..5944)
                     /gene="panF"
                     /locus_tag="ACHOQW_001742"
     CDS             complement(4493..5944)
                     /gene="panF"
                     /locus_tag="ACHOQW_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP9572829.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5934..6176)
                     /gene="yhdT"
                     /locus_tag="ACHOQW_001743"
     CDS             complement(5934..6176)
                     /gene="yhdT"
                     /locus_tag="ACHOQW_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP9572830.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6285..7634)
                     /gene="accC"
                     /locus_tag="ACHOQW_001744"
     CDS             complement(6285..7634)
                     /gene="accC"
                     /locus_tag="ACHOQW_001744"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9572831.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7645..8115)
                     /gene="accB"
                     /locus_tag="ACHOQW_001745"
     CDS             complement(7645..8115)
                     /gene="accB"
                     /locus_tag="ACHOQW_001745"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9572832.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9093..10067)
                     /gene="yhdH"
                     /locus_tag="ACHOQW_001746"
     CDS             complement(9093..10067)
                     /gene="yhdH"
                     /locus_tag="ACHOQW_001746"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP9572833.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10219..12159
                     /gene="csrD"
                     /locus_tag="ACHOQW_001747"
     CDS             10219..12159
                     /gene="csrD"
                     /locus_tag="ACHOQW_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP9572834.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12464..13507
                     /gene="mreB"
                     /locus_tag="ACHOQW_001748"
     CDS             12464..13507
                     /gene="mreB"
                     /locus_tag="ACHOQW_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP9572835.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13573..14676
                     /gene="mreC"
                     /locus_tag="ACHOQW_001749"
     CDS             13573..14676
                     /gene="mreC"
                     /locus_tag="ACHOQW_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9572836.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            14676..15164
                     /gene="mreD"
                     /locus_tag="ACHOQW_001750"
     CDS             14676..15164
                     /gene="mreD"
                     /locus_tag="ACHOQW_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9572837.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15173..15766
                     /gene="yhdE"
                     /locus_tag="ACHOQW_001751"
     CDS             15173..15766
                     /gene="yhdE"
                     /locus_tag="ACHOQW_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP9572838.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15756..17225
                     /gene="rng"
                     /locus_tag="ACHOQW_001752"
     CDS             15756..17225
                     /gene="rng"
                     /locus_tag="ACHOQW_001752"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP9572839.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17293..21093
                     /gene="yhdP"
                     /locus_tag="ACHOQW_001753"
     CDS             17293..21093
                     /gene="yhdP"
                     /locus_tag="ACHOQW_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP9572840.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21249..22694
                     /gene="tldD"
                     /locus_tag="ACHOQW_001754"
     CDS             21249..22694
                     /gene="tldD"
                     /locus_tag="ACHOQW_001754"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP9572841.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22828..23757)
                     /gene="aaeR"
                     /locus_tag="ACHOQW_001755"
     CDS             complement(22828..23757)
                     /gene="aaeR"
                     /locus_tag="ACHOQW_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP9572842.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23940..24143
                     /gene="aaeX"
                     /locus_tag="ACHOQW_001756"
     CDS             23940..24143
                     /gene="aaeX"
                     /locus_tag="ACHOQW_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP9572843.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24151..25080
                     /gene="aaeA"
                     /locus_tag="ACHOQW_001757"
     CDS             24151..25080
                     /gene="aaeA"
                     /locus_tag="ACHOQW_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP9572844.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25086..27053
                     /gene="aaeB"
                     /locus_tag="ACHOQW_001758"
     CDS             25086..27053
                     /gene="aaeB"
                     /locus_tag="ACHOQW_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP9572845.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27145..27417
                     /gene="yhcO"
                     /locus_tag="ACHOQW_001759"
     CDS             27145..27417
                     /gene="yhcO"
                     /locus_tag="ACHOQW_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP9572846.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27473..27736)
                     /gene="yhcN"
                     /locus_tag="ACHOQW_001760"
     CDS             complement(27473..27736)
                     /gene="yhcN"
                     /locus_tag="ACHOQW_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP9572847.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6659 a         6951 c         7410 g         6767 t
ORIGIN      
        1 tcgtataaat acacattagg tgatagatta accttcgcta ttttctcact ctgtgtcgaa
       61 gatatttatt tcctgaataa ttaatcatgg caaaaagaac caaagccgaa gctctgaaga
      121 cccggcaaga actgattgaa actgccatcg cccagtttgc gcaacatggc gtaagcaaga
      181 cgacgctcaa cgacattgcc gatgccgcta acgttacgcg tggcgctatc tactggcact
      241 tcgagaacaa gacccaactg tttaatgaga tgtggttgca acagccttca ttgcgggagt
      301 taatccagga acacttgacg gctggattag agcatgaccc gtttcaacaa ttgcgtgaaa
      361 aattgattgt cggcttgcaa tatattgcca aaattccccg ccagcaggcg ttgctgaaaa
      421 tcttatatca caaatgtgaa tttaatgatg agatgctggc cgagggagtg atacgcgaaa
      481 agatgggctt taatccgcag actctccgcg aagtattgca ggcgtgtcag caacaaggtt
      541 gtgtagcaaa taacctcgat ttagatgttg tgatgattat tattgatggt gccttcagcg
      601 gaattgttca aaactggtta atgaatatgg cgggttatga tctttataaa caagcccccg
      661 ctctggtcga taacgtatta agaatgttca tgccagatga aaacataacg aaattaattc
      721 atcaaacgaa tgaattaagt gtcatgtaaa ttcacattct gtcctgaaaa ttcagttcgt
      781 cacagttaac cagttttagt ggattaactg agttcatatt gcggcatttg tctgtttctg
      841 ttgccagcag ttcaatccac tgcatcagta aagtatcaaa cagaaaaacg aagacagcga
      901 aaacgacgag ccaccaatac ttgcgaatca ttacctgagc ctgtgcatgt ttaaccaagt
      961 ctggcaggaa atatacacga aatgaataat ctgcaaacca ggcttacaca tcctttactt
     1021 tgtaatgaga tcggggtcaa cttcgcttaa ccggcttcgt tttgacaggt tgccggtctt
     1081 tctttttttc actttcgcca gttcttccgc agagtaatgc gacgcgacat ccagccgtcg
     1141 aagccccatt ttgatgtact cgctgttgat ctcaatacca atgaattttc gtccgctggc
     1201 gatggctacg gcaccggtag taaagctacc agcaaacggg tcgagaacga tatcgcctgg
     1261 gttggaagag gcgagaataa tgcgtttcag taaggcttcc ggtttttgcg tcgggtggtt
     1321 ttcatattca tccatcaaat aacgcacgcg cggaaaatcc cagacgttac ccggtacttt
     1381 ttgatgattg taaggctgtg gaggattttt gcgataatcg atcaacgcgc gctgcgatcc
     1441 ggttttggct tctaccagaa tagcatcacc gttgaatgtg tagttctttg cgtctttcac
     1501 catcatcagg atgggttcgt acatggagcc gtagtgtttt ttcgcctgca ctccagaact
     1561 gtcatatgac cagacgatgc gacttttgat ggtaaaaagc ttgcggcact ggagatcgat
     1621 aaagggcatg ttttccgtac tgttcatgat gtacatgctg ccctgctttt tcagaacgcg
     1681 gtggcactct gcaatcactt ctaacagcca gtcgataaac agatcttctt tccaggcttc
     1741 gatcagacca tcaaaatttt taccgatgtt atacggtggg tcggcaaaga tcagatcgac
     1801 gctttcagta ggtagctttt taagttcggc aagggcatca ccgtgaataa tggtcttagc
     1861 ttcattacca aaccgggtcg gttcacatcc tgttctcatg gtcactccct ttgtgacacc
     1921 tataaaaaag gcgcttcccc atgccgagta gcgcctttta atcaagcaat tagctaacct
     1981 gaattagttc atgccgtatt ttttcaattt tttacgcagc gtaccacggt tgatgcccat
     2041 catcagcgca gcacgggtct ggttaccacg ggtgtattgc atcaccatgt ccaacagggg
     2101 ctgttctact tcagccagta ccagctcata gaggtcattc acatcctgac cattcagttg
     2161 agcaaaatag ttcttcagtg cctgtttaac cgagtcacgc aggggttttt gggttacctg
     2221 atcctgagag ttaacggtag aaacggtcag tacgtcagaa tttacgcgtt gttcgaacat
     2281 agttctgtca gctctttatt tctgtttacg caaaattttc gaagtatgcc tccaacgcct
     2341 ccagctgttc gctggcatcc tcaatggcgt tgaatgtgcg ccgaaactgg tcatttggag
     2401 cgtgttcctg gagataccag gaaacgtgtt tacgtgcaat tcggtaccct tttgccggac
     2461 cataaaagtc atgcagttcc cgaacgtgcg cgcaaagcaa gcgcttaacc tctgccaaag
     2521 gcagcggggg cagcaactcc ccagtgtcca gataatgctg gatttcccga aagatccagg
     2581 gtcttccctg agctgcgcgg cctatcatca gggcatccgc ccctgtatag tcgagcacag
     2641 ctctggcttt aagcgggtca gtaatgtcgc cattcgcgat aaccggaatg gaaactttct
     2701 gcttaactgc ccgaatactg tcgtactcag cttctccatt gaacaaacag gcgcgtgtac
     2761 ggccatgaat ggtcagagcc tgaatgccac agtcttcagc cagttgggca atctcttcgc
     2821 agttacggtg ttccggtgcc cagccggtgc gaatcttcag ggtaacagga acgtccactg
     2881 cattgacgac ctcggtaagg atcgatttaa cgacatccgg gtactgcaag agggctgaac
     2941 ctgcgagctt gcgattcact tttttagccg ggcaacccat attgatatca ataatctggg
     3001 caccgctttc cacgttaata cgtgctgcat ctgccatttc tttcggatcg ctaccagcaa
     3061 tttgcacggt gcgaataccg ggttcatcaa tgtgcaccat ccgtaaacga gatttgtcgc
     3121 tttcccaaac ctgtgggtta gaagacatca tctcggatac tgtcaatccg gctcccatct
     3181 cgtagcacaa cgtccgaaaa ggtctgtctg taatgccagc catgggcgct gcgatcaggc
     3241 gatttctgag ctgatattgt ccgatgcgca tgagttaaga aatgaccata ctgtgactgc
     3301 aaggcggcgt atattacgca ttttttgcac gagatgaaag gccaaacttt gaccaatcct
     3361 ctgagatgga tcaaagaatt gcatttaaaa tgagcgtggt gcgataatta ctcataaaaa
     3421 tcatcatatt agaaaatagt gactaaaatt tacactcaaa gaaatttgag taagttctca
     3481 atttttcttt atgaatgaaa attttggcac gcaaatttgc gtaaataatc ggcaaattta
     3541 cgtgcctttt gtgagcttgc tcgcacttcg ccccgcgtca ccctatggcg atgcgaaggt
     3601 taattcttac gaccggtaat acggcaccac tcttcttttt ccacgaccgg gtccagagcg
     3661 aagctgtcgg cataggcttc gcaaacgctc tccgcctggc ttgccagaat accagaaagg
     3721 cccagtaagc cgcctgaaac tggcaggacg ctaattaacg gtgccagttc acgtaatggg
     3781 cctgcaagga tgttagcgac caccacgtcg gctttcattt cttctggctg atctttcggt
     3841 aagtagagtt ccagacggtc agaaacgcca ttacgttcgg cgttatcgcg gctggcctga
     3901 atcgcctgcg gatcgatatc aataccaatg gcttttgctg cacccagttt cagcgccgcg
     3961 atcgccagaa tgccggaacc acagccaaag tcgatgactg ttttaccggt taaatcgagg
     4021 ctgtcgagcc attgcaggca cagagaggtg gttggatggg tacccgtacc aaacgccagc
     4081 cctggatcta acatcacgtt gacggcgttt tcgtccggca catcacgcca gctagggcag
     4141 atccacagtc gttcgccaaa gcgcatcggg tggaaattat ccatccattc gcgctcccag
     4201 tctttatctt ctagttgttc gattttatgc gcgaagcctg cgccgagcag cggatggttt
     4261 tccagaatcg ccaccacgtc gttcatatcg gtttcagcgt cgaacagacc aatcacatcg
     4321 gtgtcgcccc acaggcgcgt ttcgcccggc agcggctcaa atactggcgt atcgtgggta
     4381 tcctgaaaag tgatagaaac ggcacccgct tccatcagcg catcgctaag atcttccgcg
     4441 ttcgcgccgg tggtgttcag tttcagttgg atccaaggca tggcaaaact ctttatttat
     4501 cagtagtcaa aacggtagct tgcgggacgg atgtaccgaa acggtttccg accaggaaag
     4561 ccagcaaact tagtagtaac gagggcacga tagggtggaa gcccaggtac tgaatattca
     4621 gcgtcgcgag tacggcatac agcacgccgc caacgatcat cgcacttagc gcgcctttgg
     4681 cgttggcgcg ttcccagtaa agacccagca ccagcggcca caggaaaacg gcttccagcc
     4741 caccgaaggc cagcaaattc agccagatga tcatttctgg cggcttccag gcggcaagca
     4801 gcagcaacgc gccgagaact aacgtaatta ccgccgacat ccgcttcaga cgcgtctcgt
     4861 tttgcatttg atccggacgg atattcagat agagatcttt aatgatcgta gcggaacttt
     4921 gtagcaattg ggcgttaatt gtcgacatga tcgcagccat aggtgcagcc agaaagatcc
     4981 cggcagcaaa cggtggcagc acttttacca ttaacgtcgg gatcaccaga tccggtacgg
     5041 tgagatcggg gatcactgca cgacctaacg ctccggctag gtgcataccg aacatcagga
     5101 ttgcgactac aatcgtaccg atgatgatcc cccggtgtac tgctttgctg tctttataag
     5161 agatacagcg caccgcagta tgcggcaggc ctataacgcc aaagcacacc agtacccaga
     5221 acgacgtcat aaaggcaggc gacagaatat cgtcagcgcc ttgtggcgta accagttgcg
     5281 gatcgatggt ttgcaaggtc tgtactgcgt tgcttaagcc gccagcggca tgtaccacgc
     5341 caataagcag cacaacggtg ccaatcagca tcacaagccc ttgcatggtg tcgttcagca
     5401 cgctggcgcg aaagccacca aaggcggtat ataacgcaat gctgatacca aaaatcagca
     5461 gcccggtttc ataaggaata cccgccgcgg tttccagcag gcgcgcaccg ccgataaact
     5521 gcacggtcat cgcaccaacg aacgcaacca gcaaactcaa actcgccagc cacaccagaa
     5581 gacgactctg gtagcgggca aacagcatat cgttcagcgt cactgcattg tagcggcgcg
     5641 caagaatcgc aaacttcttg ccgagaatac cgagtgaaag ccagactgcc ggaagctgaa
     5701 tcatcgccag caatacccag ccaaggccgt atttgtaagc agctccaggc ccgccgataa
     5761 acgaactggc actgatataa gtcgcggtga gcgtcatcgc cagcacaata ccgcccatag
     5821 agcggctgcc gaggaaatac tcattaagga aggtgccggt gctccgttta cgcatcgcat
     5881 aaaccgagat accgaacacc accaccagat aggcgaccag cggtagaatt acttcaagct
     5941 gcatcgtcat cctccagcgg gatatcgcga tagataaatt tcaccatcgc ccagcacagt
     6001 ccaataaaca gcagcggcgt caggatgcag gccatctcaa accagcgcgg aaagccggta
     6061 aaaccggggg caacgccagg taagtaagcg gctactaacc aaactgccag atacaaaagg
     6121 gtcagcccca gcgcccagcg cgcctcttta tgggcctgaa caaaacgagt gtccattttt
     6181 tgtccctgat gggtgaagaa agcgaggatt gtaccttatg ggtgttgtcg atccccagta
     6241 ataaaaaagg ccggaaaatc cggccttttg acgcttttgc agtcttattt ttcctgaaga
     6301 ccgagttttt tctccagata gtggatgtta gtgccaccat gctggaagtt ctcgtcattc
     6361 atgatgcgga tctgcagatc aacgttggtt ttgataccgt cgatgatcag ctcctgcagc
     6421 gcattcttca tgcgggcaat cgccacgtca cggttttcac cgtagcaaat cagcttaccg
     6481 atcattgagt catagtacgg cggtacggtg tagcccgcgt agatatgaga ctcccaacgt
     6541 acgccaaaac cgccaggtgc gtggaaacgg gtgattttgc ccggacttgg caggaaggtg
     6601 ttcggatctt cggcgttgat acgacattcc accgcatggc cgcgaacgtg aacttcttct
     6661 tgcttgatcg acagcggttg accggcagcg atacgcagct gttctttgat caggtcaacg
     6721 ccggtgatca tttctgtaac cgggtgttct acctgaatac gggtgttcat ttcgatgaaa
     6781 tagaactcgc cgttttcgaa caggaactcg aaagtacctg caccgcgata gccgatatca
     6841 acacacgctt tagcgcaacg ttcgccgatg tagcgacgca gttccggggt aatgcccggt
     6901 gctggtgctt cttcgaccac tttctggtgg cggcgctgca tggagcagtc acgttccgcc
     6961 agatagatag agttgccctg accgtcagcc agtacctgaa tctcgacgtg gcgaggattt
     7021 tccaggtatt tctccatgta aaccatatcg ttgctgaaag cagctttcgc ttccgcacgg
     7081 gtcatggaga tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat accgcgacca
     7141 ccgccgccgc cggaggcttt gataatcacc ggataaccaa tgcgtttagc aatggcacgg
     7201 tttttatcca tatcgtcgcc cagcgggccg tcagaacccg gtacgcaagg gacgcccgct
     7261 tttttcatcg ccgcgattgc ggatactttg tcgcccatca ggcgaatggt ttctgctttc
     7321 gggccaatga agataaagcc ggagcgttca acctgctcgg caaagttggc gttctcggag
     7381 aggaagccgt aacccggatg gattgctact gcgccggtga tttcagcggc gctgatgatt
     7441 gccgggatgt tcagataact ttttactgac ggagcagggc caatacagac cgtttcatct
     7501 gccagtaata cgtgttttag atcgcgatcc gcgctggagt gcacagcgac agtcttgatg
     7561 cccagttctt tacaggcacg aagaatacgc aatgcaatct cgccgcggtt ggcaataaca
     7621 attttatcca gcatgttcgc ctcgttactc gatgacgacc agcggctcgt caaattctac
     7681 cggttgtcca ctttcgacca gaattgcttt cacggtaccg gatttgtccg cttcgatctg
     7741 gttcatcatt ttcatggctt caacgatgca cagggtatcg cccacgttga ctttctgacc
     7801 cacttcgatg aacgcttttg catccgggct tggggtgcgg tagaaagtac caaccatcgg
     7861 ggaacgtacg atgtgaccac tgatttccgc tgctgctggc gcttccatgg aaggaacggt
     7921 cgccggagcg gctgcgttag attgagctgg ctgctgcatc attggtgcag cgtaagcttg
     7981 ttgcatcaca gggaaacttg cggcaggagc tgcacggcta atgcgtactg actcttcgcc
     8041 ttcagaaatt tccagttcgg agatgcctga ttcttcaacc agctcgatca gttttttaat
     8101 cttacgaata tccatgagtg ggttccgtac tctttgttta gtgtgattgt gacaggcgtt
     8161 tcaccgccgt ctgtaaagcg tatgccccga cagctgtatg catagcgata aattccagca
     8221 ggtcgggacg ccggtctatt ttgccccaga gtgtcaatgt tagacttgac ggacattgtg
     8281 cagcgatctg ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc gccaatgtca
     8341 ccttttccgc agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta taacgatttc
     8401 gtagcaattg gcagctaaat actggtctta tcagggaaga taatcaacaa ctaacatgta
     8461 aataaccttc aacaccgtgt aatttgcaac aagccgcaca attcacgaaa ttagcgccat
     8521 catcgacgga acttcttata acgtaaggtg aaaagctgta aagccagccc tgcgaagatg
     8581 aggggctgca atgagataat cgtcacagac cacaaataat gtagggatgc caggatcgcg
     8641 acaagataga cgacattacg cagcggttgc tggggattac ccaattttca ctgcatcgcc
     8701 tggatagatg tgaatgctaa agcacgcaaa attaaccgat tgacaatgct cagcattaaa
     8761 taggttcgag tgattaattt gtagtcaaac aaaatcagat tattcacctc atggtaccag
     8821 aaataagtaa ggggttaagc gtagcgtcgc ccaggcaaag caccataaac ctaacaggcg
     8881 acggaggcgt atcaataata gctgtttagt ggagtatcaa tgcgcatcaa tttatggtca
     8941 gtcgaaatca tgactttcca gtttggatga tgttgtacaa cttcatgacc aaggtgttcg
     9001 acgaaagctg tccggctaac cgcttttccc gttaaccgat tgaataatga tttcaaaatg
     9061 agttccctct cttattattc cttgctaaat ggttagttaa ccttcaccag cgtgcgaccc
     9121 tggatctggt tattaatgat ggcctcggca aagttcggtg cctctgacag agatatctct
     9181 tttgccgcct gggtatagaa tgattccggt aaatcggcga ccagtcgctg ccaggcttgt
     9241 gcgcggcgtt ctggtggcgt cattactgaa tccacccctt gcaaacggac attacgcaga
     9301 ataaatggca tgaccgtggt tggcagagta aaaccacccg ccagaccaca ggccgccacg
     9361 cagccgccgt aattcatttg cgccagcact tttgccagca ctttgtcgcc aacagtgtca
     9421 attgccccag cccagacttg tttttccaga ggacgggatt cggcaaactc atcacgaggg
     9481 agaatacggc tggcacctaa acttttcaga tattcatggg tactttcgcg accggaaacg
     9541 gcaacgacct gataacccaa cttatgcagc agcgccacgg cggtactgcc gacgccacca
     9601 ctggcacccg tcacgacaat ctccccgtcc tgcgggcgaa caccggcatc ttccagcgcc
     9661 atcacacaca gcatggcggt aaaaccggca gtaccgataa tcattgcttt acgcgcgtcc
     9721 agcccttgcg gcatggcaac cagccagtca cctttcactc gcgcctgctc cgccagccca
     9781 ccccagtggt tttcaccaac gccccagcca gtgagtaaca cctcctgacc ggcatgaaaa
     9841 cgcggatctt cgctggtgcg tacagttccg gcaaaatcga tcccaggaat catcggaaaa
     9901 ttacggatga tttttccctt accggtaatc gccagcgcat ctttatagtt caggctcgac
     9961 cagtgaacat cgaccgtgac atcgccctcc ggcaggcgac tttcgtccag agtctgtact
    10021 gatgcgagag ttttgccgtc ctgctgttct aaaagtaacg cctgcataag tggtcctcat
    10081 gtgcatgatg gattggaaaa taatttctga agactatact cgctaatgga aaagcaaagc
    10141 cgatgaagcg caataaattg cgagataaat ctgatttgct agtatgcccg cttcctcact
    10201 atcggagtta acacaaggat gagattaacg acgaaatttt cggcctttgt tacgctgctc
    10261 accgggttaa caatttttgt gactttgctg ggctgttcgc taagtttcta caacgccatt
    10321 cagtataagt ttagtcatcg cgttcaggcg gtggcgacgg cgattgatac ccaccttgtg
    10381 tcgaatgact tcagcgcatt aaggccacaa attaccgaat taatgatgtc ggcagatatc
    10441 gttcgtgtag acctgctcca tggtgataag caggtttata ccctggccag aaatggtagt
    10501 tatcgtccgg ttggcaccag cgatctgttt cgtgaactga gcgttccgtt gataaagcat
    10561 ccggggatgt cgttgcgtct ggtttatcag gatccgatgg gcaactattt ccattcgttg
    10621 atgaccaccg cgccgctcac aggggcgatt ggctttatca ttcttatgct ctttctggcg
    10681 gtacgctggt tacaacggca acttgccggg caagaattgc tggaaacccg ggctactcgt
    10741 atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg gccgcccaga
    10801 accagcagtg cgctggatac gctgcttcgt gaaattcaga acgcacgcga acaacacagc
    10861 cgtcttgata cgctgatccg ctcttatgcc gcacaggacg tgaaaaccgg cctcaataac
    10921 cgactctttt tcgataatca gttagcaacg ttactggaag atcaggagaa agtaggtacc
    10981 cacgggatcg tgatgatgat tcgtctgccg gatttcaata tgttgagcga tacctggggg
    11041 cacagccagg ttgaagaaca gttcttcact ctgacgaatc tgctgtcgac atttatgatg
    11101 cgctaccctg gcgcactgct ggcgcgttac caccgcagtg attttgctgc gctgttaccg
    11161 caccggacgt taaaagaggc agagagcatc gccggtcagt taatcaaagc cgtcgatacc
    11221 ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc acattggtat ctgcgcctgg
    11281 cgtagtggtc aggataccga gcaggtaatg gaacatgcag agtctgccac gcgtaatgcg
    11341 ggattgcagg gcggcaatag ctgggctatt tacgatgact cgttgcctga aaaaggacgc
    11401 ggtaatgttc gctggcgtac gcttatcgag caaatgctca gtcgcggcgg cccgcgcctt
    11461 tatcaaaaac cggcggttac tcgcgaaggt caggttcatc atcgcgaact catgtgccgc
    11521 atctttgatg gtaatgaaga ggttagctcg gcggagtata tgccgatggt cttgcagttt
    11581 ggcttatcgg aagagtatga ccgtctgcaa atcagccgtc ttattccact attgcgttac
    11641 tggccagagg aaaatctggc gattcaggtt accgttgagt cgctgattcg cccgcgtttt
    11701 cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat cacaacgaaa acgcataatt
    11761 attgaacttg cagaggccga tgtaggtcaa catatcagtc gtttacaacc tgttattcgt
    11821 ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg ctggtttgac gctggtaagc
    11881 accagttgga tcaaagaact taatgttgag ttactcaagc tccatccggg gctggtcaga
    11941 aacattgaga agcgaacgga gaaccagctg ctggttcaaa gcctggtgga agcctgctca
    12001 gggaccagca cccaggttta cgccaccggt gtgcgttcgc gaagcgagtg gcagaccctg
    12061 attcagcgcg gtgttacagg cgggcaaggg gattttttcg cgtcctcaca gccacttgat
    12121 actaacgtga aaaaatattc acaaagatac tcggtttaac ctgccgttta atccgttttc
    12181 acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc cagattgttg
    12241 cagcacatat gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa tgctgctgat
    12301 gcattaagcc tttctggact caggcagaga tttgtaacaa aggaaacgaa ctgcactaat
    12361 tttcaccgta gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg gtaaagtaag
    12421 cggattttct tttccgcccc agctttcagg attatccctt agtatgttga aaaaatttcg
    12481 tggcatgttt tccaatgact tgtccattga cctgggtact gcgaataccc tcatttatgt
    12541 aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc aggatcgtgc
    12601 cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc tgggccgtac
    12661 gccgggcaat attgctgcca ttcgcccaat gaaagacggc gttatcgccg acttcttcgt
    12721 gactgaaaaa atgctccagc acttcatcaa acaagtgcac agcaacagct ttatgcgtcc
    12781 aagcccgcgc gttctggttt gtgtaccggt tggcgcgacc caggttgaac gccgcgcaat
    12841 tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag aaccgatggc
    12901 tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg tggttgatat
    12961 cggtggtggt accactgaag ttgctgttat ctccttgaac ggcgtggttt actcctcttc
    13021 cgtacgcatt ggtggtgacc gtttcgacga agctatcatc aactatgtgc gtcgtaatta
    13081 cggttctctg atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg gttcggctta
    13141 tccgggcgat gaagtccgtg aaatcgaagt tcgtggccgt aacctggcag aaggtgttcc
    13201 acgcggtttt accctgaact ccaacgaaat cctcgaagca ctgcaggaac cgctgaccgg
    13261 tattgtgagc gcggtaatgg ttgcactgga acagtgcccg ccggaactgg cttccgacat
    13321 ctccgagcgc ggcatggtac tcaccggtgg tggcgcactg ctgcgtaacc ttgaccgttt
    13381 gttaatggaa gaaaccggca ttccagtcgt tgttgctgaa gacccgctga cctgtgtggc
    13441 gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc tgttcagcga
    13501 agagtaatcg gatgcaggca ggggaagcgt ctgtttaccc tgcctggtct gatacgagaa
    13561 tacgcataac ttatgaagcc aatttttagc cgtggcccgt cgctacagat tcgccttatt
    13621 ctggcggtgc tggtggcgct cggcattatt attgccgaca gccgcctggg gacgttcagt
    13681 caaatccgta cttatatgga taccgccgtc agtcctttct actttgtttc caatgctcct
    13741 cgtgaattgc tggatggcgt atcgcagacg ctggcctcgc gtgaccaact agaacttgaa
    13801 aatcgggcgt tacgtcagga actgttgctg aaaaacagtg aactgctgat gcttggacaa
    13861 tacaaacagg agaacgcgcg tctgcgcgag ctgctgggtt ccccgctgcg tcaggatgag
    13921 cagaaaatgg tgactcaggt tatctccacg gttaacgatc cttatagcga tcaagttgtt
    13981 atcgataaag gtagcgttaa tggcgtttat gaaggccagc cggtcatcag cgacaaaggt
    14041 gttgttggtc aggtggtggc cgtcgctaaa ctgaccagtc gcgtgctgct gatttgtgat
    14101 gcgacccacg cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat tgcagccggt
    14161 aacggttgta cggatgattt gcagcttgag catctgccag cgaatacgga tattcgtgtt
    14221 ggtgatgtgc tggtgacctc cggtctgggc ggtcgtttcc cggaaggcta tccggtcgcg
    14281 gttgtctctt ccgtaaaact cgatacccag cgcgcttata ctgtgattca ggcgcgtccg
    14341 actgcagggc tgcaacgttt gcgttatctg ctgctgctgt ggggggcaga tcgtaacggc
    14401 gctaacccga tgacgccgga agaggtgcat cgtgttgcta atgaacgtct gatgcagatg
    14461 atgccgcagg tattgccttc gccagacgcg atggggccaa agttacctga accggcaacg
    14521 gggatcgctc agccgactcc gcagcaaccc acgacaggaa atgcagctac tgcgcctgct
    14581 gcgcccacac agcccgctgc taatcgctct ctacaaaggg ctacgccgcc gcaaagtggt
    14641 gctcaaccgc ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt agccagggac
    14701 gctgggtaat ctggctctct ttcctcattg cgcttttgct gcaaatcatg ccctggccgg
    14761 ataacctgat tgttttccgg ccaaactggg tgttactcat cttgttgtat tggatcctgg
    14821 ccttgcccca tcgcgtaaat gtgggcacag gttttgtgat gggtgccata ctggatctga
    14881 tcagcggctc gacgcttggc gtacgcgtat tggcgatgag catcattgct tacctggtgg
    14941 cgctgaaata ccagcttttc cgcaacctcg cattatggca gcaggcgctg gtcgtcatgt
    15001 tgctttcgct ggtggtggat attattgttt tctgggcaga gtttttagtg attaacgtct
    15061 ctttcagacc ggaagtgttc tggagtagtg tagtcaatgg ggtgctctgg ccgtggattt
    15121 tcttgctgat gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt ctatgacttc
    15181 tctgtattta gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac ttggcgtgac
    15241 ctttgaacgt attgttacgg gcattgagga gcagcgtcag ccgcaggaga gcgcgcagca
    15301 gtatgttgtg cgtctggcgc gcgagaaagc acgggcaggt gtcgcgcaaa cggcgaagga
    15361 tctcccggtg ctgggtgcgg atactatcgt tatcctgaac ggagaagtgc tggagaaacc
    15421 gcgcgacgca gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga cccatcaggt
    15481 gatgacggca gtggcgttgg ccgacagccg gcacattctc gattgcctgg tggtcaccga
    15541 tgtgactttc agaacgttaa cagacgaaga catcgcgggc tatgtcgcca gcggtgaacc
    15601 gttagataaa gcaggtgcat acggtattca ggggctgggt ggctgttttg tcaggaagat
    15661 aaatggcagc tatcaagccg tagtcggctt accgctggtt gaaacgtatg aattattaag
    15721 taattttaac gcactgcgtg agaaaaggga taaacatgac ggctgaattg ttagtaaacg
    15781 taacgccttc ggaaacgcga gtggcgtata ttgatggcgg tattctgcag gaaattcata
    15841 ttgaacgtga ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt gtaagtcgtg
    15901 tacttccggg tatgcaggcg gcttttgtag atattgggct ggataaagcc gcgtttcttc
    15961 atgcatctga catcatgccg cacaccgaat gtgtggcggg tgaagaacaa aagcaattca
    16021 cggtgcgcga catctcggaa ctggttcgtc aggggcaaga tctgatggtg caggtggtga
    16081 aagatccgct tggcactaag ggggcgcgcc tgaccaccga tatcacgctg ccttctcgct
    16141 atctggtctt tatgccaggg gcttctcacg ttggggtttc ccaacgaatt gaaagcgaat
    16201 cagaacgtga acgcctgaaa aaagtggtcg cagagtattg cgacgagcag ggcgggttta
    16261 tcatccgtac cgcagcggaa ggggttggcg aggctgaact ggcctccgat gccgcttatc
    16321 tgaaacgcgt ctggaccaaa gttatggagc gtaaaaagcg cccgcagacc cgttatcagc
    16381 tgtacggcga actggcgttg gcgcagcgtg ttctgcgtga tttcgccgat gccgaactgg
    16441 accgcattcg cgttgactca cgcctgactt acgaagcgtt acttgagttc acctcggagt
    16501 acattcccga gatgaccagc aagctggagc attacaccgg acgccagccg attttcgatc
    16561 tctttgatgt cgaaaacgaa atccagcgag cgctggaacg caaagttgaa ctgaaatccg
    16621 gtggttatct gattatcgac cagaccgaag cgatgaccac cgtggacatc aataccggag
    16681 cgtttgtcgg tcatcgcaat ctggacgaca ccattttcaa taccaatatt gaagcgacgc
    16741 aggctatcgc tcgccagtta cggttgcgta atctgggcgg gattatcatt attgatttca
    16801 tcgatatgaa taatgaagat caccgccgcc gagtgctgca ctcgctggag caggcgttga
    16861 gcaaagaccg ggtgaaaacc agcgttaatg gtttttcggc gctggggctg gtggagatga
    16921 cgcgtaaacg cacccgcgaa agcattgagc acgtactgtg taacgaatgc ccaacctgcc
    16981 acggtcgcgg aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg cgcgagattg
    17041 ttcgtgtaca ccatgcttac gactccgacc gtttcctggt ctatgcttct ccggcagtag
    17101 ccgaagcctt gaaaggcgaa gagtcacact cgctggcgga agtggaaatt ttcgttggca
    17161 aacaggttaa agtacaaatt gaaccgctct ataaccagga gcagtttgac gtcgtaatga
    17221 tgtaaacaga tgctgggccg ccatccggca aagggttttt gagtcacatt tttagcagac
    17281 aaggagtgac gggtgaggcg attgccgggg attttactgc ttactggagc cgcgctcgtt
    17341 gtgatcgctg ccctgctggt tagcggcctg cgtattgctt taccgcatct tgacgcctgg
    17401 cgtccggaaa tcctcaacaa aatagaatcc gcgactggca tgccggtaga agccagtcag
    17461 ctctcagcca gctggcagaa ttttggcccg acgcttgaag cgcacgacat ccgtgcagaa
    17521 ctaaaggatg gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga tgtctggcag
    17581 agcctgttac atatgcgctg gcagtttcgc gacctcactt tctggcagct gcgctttcgc
    17641 accaacactc ctatcaccag cggtggtagc gatgacagtc tggaagccag tcacatcagc
    17701 gatctgtttc ttcgtcaatt tgaccatttc gatctccgcg acagtgaagt cagtttcctg
    17761 acgccatccg gtcagcgcgc cgagctggcg atcccacaac tcacctggct gaacgatcca
    17821 cgtcgacacc gtgcggaagg cctggtaagc ctctccagcc ttaccggaca gcacggcgtg
    17881 atgcaggtgc gcatggattt gcgcgatgat gaggggttgt taagcaatgg tcgcgtctgg
    17941 ctccaggcgg atgacatcga cctgaagccg tggctcggta aatggatgca ggacaatatt
    18001 gcgctggaaa cggcgcagtt ctcccttgaa ggctggatga cgatcgacaa aggcgatgta
    18061 accggcggtg acgtctggct gaaacagggt ggtgccagct ggttgggcga gaaggaaacg
    18121 catacgctgt cggtggataa tctgaccgcg catattacgc gtgaaaatcc gggatggcag
    18181 ttctctattc ccgatacacg gatcacgatg gacggcaaac cctggccgag cggagcattg
    18241 acgctggcct ggataccgga acaggacgtt ggcggcaaag acaataaacg cagtgacgaa
    18301 ctccggattc gcgccagtaa tctggagctg gcaggcctgg agggcatacg cccgctggcc
    18361 gcgaaacttt cacctgcact gggtgatgtt tggcgctcta cacaaccgag cggcaagatt
    18421 aacactctgg cgctggatat cccgcttcag gcggcagaca agacccgttt tcaggcatcg
    18481 tggagcgatc tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca cttctccggg
    18541 acgctttccg gcagcgttga aaatggtttg cttaccgcgt cgatgaagca ggcaaagatg
    18601 ccttacgaaa cggtattccg tgcgccactg gaaatcgccg acggccaggc aactataagc
    18661 tggctgaaca atgacaaagg tttccagctg gatgggcgta atattgacgt taaagccaaa
    18721 gccgtccatg cgcgcggcgg ttttcgttac ctgcaacctg ctaacgatga accctggctg
    18781 ggtattctgg ctggcatcag taccgatgat ggttcacaag cctggcgcta tttcccggaa
    18841 aacttgatgg gtaaagacct ggttgattac ttaagtggcg cgattcaggg cggtgaagcg
    18901 gataacgcga cgctggttta tggtggcaat ccgcaactct tcccctataa acacaacgaa
    18961 ggtcagtttg aagtgctggt gccgctgcgc aacgcgaagt ttgccttcca gccggactgg
    19021 cctgcattaa ctaaccttgg tattgaactg gactttatta acgacggttt atggatgaaa
    19081 accgatggcg ttaatctggg cggcgtgcgc gcgagtaatc tcaccgcagt gatccctgac
    19141 tactcaaaag aaaaactgct gattgacgct gacattaaag gtccgggtaa agccgttggc
    19201 ccttactttg atgagacacc gctgaaagat tctctgggtg cgaccctgca agaactccag
    19261 ctcgacggcg atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg cgagctggta
    19321 accgcgaaag gtgaagtgac gctgcgtaat aacagtctgt ttatcaaacc actcgacagc
    19381 accctgaaaa atttgagcgg taaattcagc tttatcaatg gcgatctgca aagtgaacca
    19441 ctgacagcaa gctggtttaa tcagccgttg aacgtggatt tttccaccaa agaaggggca
    19501 aaagcctacc aggtcgcggt gaatctcaac ggtaactggc aaccggcgaa aaccggcgtt
    19561 ctgcctgcag cggtgaacga agcattgagt ggcagcgtgg cgtgggatgg taaagtgggc
    19621 attgttctgc cttatcatgc tggcgcgacg tataacgtag agctaaacgg cgatttgaag
    19681 aatgtgagca gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc actgccggta
    19741 aacgttaagg ttgatggcaa tctcaacagc tttgaattaa ccggacaggc tggtgcggat
    19801 aatcatttca atagccgctg gttgctcggt caaaagctga cgctcgaccg tgctatttgg
    19861 gcggcagaca gtaaaacgct cccgccgttg ccggaacaaa gtggtgttga actcaatatg
    19921 ccgccgatga atggtgccga gtggctggcc ctgtttcaga aaggtgcggc ggagagtgtc
    19981 ggtggtgcag cgagtttccc acaacacata acgttacgta cgcctatgtt gtcgctggga
    20041 aatcagcaat ggaataacct gagtattgtt tcgcaaccga cggcaaatgg cacccaggtt
    20101 gaggcgcagg ggcgtgaaat caacgccacg ctggcgatgc gtaataacgc gccgtggctg
    20161 gcgaatatca aatatcttta ttacaacccg agcgtggcga aaactcgtgg tgattcaacg
    20221 ccgtcatcac ctttcccgac aacggaacgc attaacttcc gtggctggtc ggacgcccaa
    20281 atacgatgcg cagagtgctg gttctggggg caaaaattcg ggcgcattga cagtgatatc
    20341 accatttctg gcaatacatt aacgctgacc aatggactga ttgatactgg tttttcgcgg
    20401 ctcactgccg atggtgaatg gattaacaat ccggggaatg aacgtacctc gctgaaagga
    20461 aaactgcgcg ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac gacgcccata
    20521 cggcagtcgt catttaatgt ggattacgat ttacactggc gtaaagcacc gtggcagcca
    20581 gatgaggcga cgttgaatgg catcattcat actcaactgg gtaaaggcga aattaccgaa
    20641 atcaataccg gacatgccgg gcaattgctg cgcttattga gcgtagatgc cctgatgcgt
    20701 aagctgcgtt ttgatttcag agacactttt ggcgaagggt tctattttga ctccattcgc
    20761 agcaccgcgt ggattaaaga cggcgttatg cacaccgacg acacgctggt ggatggcctg
    20821 gaggcggata tcgccatgaa agggtcggta aatctggtac gtcgcgacct gaatatggaa
    20881 gcggttgtcg caccagagat ttctgcgaca gtgggcgtgg ctgcggcttt tgcggttaac
    20941 cccattgttg gcgcggcagt gtttgccgcc agtaaagtgc tggggccgct gtggagcaaa
    21001 gtctccattt tgcgctatca catttcgggt ccgctggacg atccgcaaat caacgaagtg
    21061 ttgcgccaac cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta attgccccaa
    21121 tctcatagga taatcgttgc caaaggccaa cgagccagaa cataaccgta ggctggatag
    21181 ggcgttcacg ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac taacgagtag
    21241 caaaaacgat gagtcttaac ctggtaagtg aacaattgct agcggcgaac ggcctgaaac
    21301 atcaggactt gttcgtgatc ctcggtcaac tggccgaacg tcgccttgat tatggcgatc
    21361 tctattttca gtcgagctat cacgaatcct gggttttaga agaccgcatt attaaagatg
    21421 gttcttacaa catcgatcag ggcgttggtg tgcgtgcaat cagcggtgaa aaaaccggat
    21481 ttgcttacgc tgaccaaatc agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca
    21541 ccatcgcccg tgatagcggt gatggcaaag tacagacgct gggcgcggta gagcatagcc
    21601 cgttgtatac ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc
    21661 tgcgtcgcgt cgataaggtt gcccgcgaag cggacaagcg cgtacaggaa gtgactgcca
    21721 gcctcagtgg tgtctatgaa ttaattctgg ttgcggccac cgacggcacg ctagcggcgg
    21781 atgtccgtcc gctggtgcgt ctttccgtga gcgttctgat cgaagaagat ggcaaacgcg
    21841 aacgcggtgc cagtggcggc ggcggtcgtt ttggttatga gttcttcctt gccgatctcg
    21901 acggcgaagt ccgtgcggat gcatgggcaa aagaagcagt acgtatggcg ctggtcaatc
    21961 tttctgccgt tgctgcacca gcgggcacca tgccggtagt acttggcgca ggttggccgg
    22021 gcgtgctgtt gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac cgccgtggca
    22081 cttcagtatt tagtggacag gtcggggagc tggtggcttc agaactgtgt accgtggttg
    22141 atgacggtac gatggtcgat cgccgtggtt cggtggcgat tgatgacgaa ggtacgccag
    22201 gccagtacaa cgtgctgatt gagaacggca ttctgaaagg ctacatgcag gataaactca
    22261 acgcgcgttt gatggggatg acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc
    22321 tgcccatgcc gcgtatgacc aacacctata tgctgccggg taaatcgacc ccgcaggaaa
    22381 ttattgaatc cgttgagtac ggtatctatg caccgaactt tggtggcggt caggtggata
    22441 tcacctccgg caaattcgtt ttctccactt cagaagcata tctgattgaa aacggtaaag
    22501 taacgaagcc ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc atgcagcaga
    22561 tttcgatggt tggcaacgac ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag
    22621 ggcaaagttt gccggttggc gtgggccagc caacgctgaa agttgataac ctgactgttg
    22681 gcggtactgc gtaataattt cagtattttc agaaggataa ttaatccttc ctcgtgcacg
    22741 aacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaacccgt tggcacaggt
    22801 ttgtacaacg taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg
    22861 aaacaattta ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt
    22921 cagcggcagc ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata
    22981 acgcggcagc aggatctcca gctccccacg attgatctcg ttgatcaccc acatcagcgg
    23041 cacgtaggcg atcccggcac ccgccgttag ccagcgcacc agcgtcatcg gatcattagt
    23101 cacaaatctc ccttgcggga tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa
    23161 ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg ctacttaaat cggcgggttt
    23221 ttccggtatg ccgtattgtg tgagatagct tttcgcggcg cacaccacca ttggcatcgc
    23281 gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc
    23341 cagaccgtcg gcaatcaggt cgggggctgg aattccggta accagattga cgctcaaacc
    23401 tgggtattct ttcagcattt tggctgtcag cccggcgaga acattttgtg ccatagttga
    23461 agaacagcca atgcgtagcg tcccgatggg ggtgttattg aaggcataca gttgctcatg
    23521 aacatcctgc acttcatgaa gcatacgacg gcagccctgg tagtaaattc taccggcttc
    23581 ggtcaggcca atgctgcgtg tgctacggtt taacagcttt acctgcaact catcttccag
    23641 ttttgacacc gtctgactga tggacgaaac gctcatctgt agctgtctgg cggcggcggt
    23701 aaaagagcca aattcaacta ctttggcaaa caccgacatg cgttttagtc gttccattat
    23761 tcactctgac ttaaaagtga tttagatcac ataatataga taacagcata acagttacgc
    23821 taatatatta aatatcaatc tacagcaatg ttgctctcgc ccggctttcc atgcccttct
    23881 ctgcggcgac agatgctgaa aataataacg cctgctctct ctatacaacc aaggtcaaca
    23941 tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt cccaccgata ttttttgaat
    24001 tgcttttatc actggcgatt ttctggctgg tgtgccgggt acttgtgcca acaggtatct
    24061 acgactttgt ctggcatccg gcgttgttca acaccgcgct ctattgctgc ttgttttatt
    24121 tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac taataagaaa attctcccgt
    24181 acggccatca cggtcgtatt agtcattctg gccttcatcg caatttttaa tgcctgggtc
    24241 tattacaccg aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc
    24301 gcgccggacg tttctggact cattacccag gtgaatgttc ataaccagct ggtgaaaaaa
    24361 ggacaggttc tgttcaccat cgaccagccg cgctatcaaa aggcgcttga ggaagcgcaa
    24421 gccgatgttg cttattatca ggtactggca caggagaaac gccaggaggc cggacgtcgt
    24481 aaccgtctcg gtgtgcaggc gatgtctcgc gaagagatcg accaggccaa caacgtacta
    24541 caaacggttc tgcatcagtt agcgaaagcg caggcgaccc gcgatctggc aaaactggat
    24601 cttgaacgca cggtgatccg cgcgccagca gatggctggg tgaccaacct caacgtctat
    24661 accggtgagt ttattactcg tggatcaacg gcggttgcgc tggtgaaaca gaactctttc
    24721 tatgtactgg cctatatgga agaaactaag ctggaagggg tgcgccctgg gtatcgtgca
    24781 gagatcacgc cgcttggcag taacaaagtg ctgaaaggga ctgttgatag tgttgccgct
    24841 ggggtcacca acgccagcag cacgcgtgac gacaaaggga tggcgaccat cgactctaac
    24901 cttgaatggg tgcgtttggc gcaacgtgtt ccggttcgta ttcgtctcga caaccagcaa
    24961 gagaacatct ggcctgcggg caccactgct acagtggtgg tcactggcaa acaagatcgc
    25021 gacgaaagcc aggattcgtt cttccgtaaa atggcccatc gcctgcgtga gtttggttaa
    25081 tcacgatgga tattttctcc attgctaacc aacatattcg ctttgcggta aaactggcga
    25141 ccgccattgt actggcgctg tttgttggct ttcacttcca gctggaaacg ccacgctggg
    25201 cggtactgac agcggcgatt gttgccgccg gtccggcctt tgctgcggga ggtgaaccgt
    25261 attctggcgc tattcgctat cgtggctttt tgcgcatcat cggcacattt attggctgta
    25321 ttgccggact ggtgatcatc attgcgatga tccgcgcacc attattgatg attctggtgt
    25381 gctgtatctg ggccggtttt tgtacctgga tatcctcgct ggtacgaata gaaaactcgt
    25441 atgcgtgggg gctggccggt tataccgcgc tgatcattgt gatcaccatt cagccggaac
    25501 cattgcttac gccgcagttt gccgtcgaac gttgtagcga gatcgttatc ggtattgtgt
    25561 gtgcgattat ggcggatttg ctcttttctc cgcgatcgat caaacaagaa gtggatcgag
    25621 agctggaaag tttgctggtc gcgcaatatc aattaatgca actctgtatc aagcatggcg
    25681 atggtgaagt tgtcgataaa gcctggggcg acctggtgcg acgcaccacg gcgctacaag
    25741 gcatgcgcag caacctgaat atggaatctt cccgctgggc gcgggccaat cgacgtttaa
    25801 aagcgatcaa tacgctatcg ctgacgctga ttacccaatc ctgcgaaact tatcttattc
    25861 agaatacgcg cccggaattg atcactgata ctttccgcga attttttgac acgccggtag
    25921 aaaccgcgca ggacgtccac aagcagctca aacgcctgcg aagagttatc gcctggaccg
    25981 gggaacggga aacgcctgtc accatttata gctgggtcgc ggcggcaacg cgttatcagc
    26041 ttctcaagcg cggcgttatc agtaacacaa aaatcaacgc caccgaagaa gagatcctgc
    26101 aaggcgaacc ggaagtcaaa gtagagtcag ccgaacgtca tcatgcgatg gttaacttct
    26161 ggcgaaccac actttcctgc attctgggaa cgcttttctg gctgtggacg ggctggactt
    26221 ccggcagtgg tgcaatggtg atgattgcgg tcgtgacgtc actggcaatg cgtttgccga
    26281 atccacgcat ggtggcgatc gactttatct acgggacgct ggccgcgctg ccgttagggc
    26341 tgctctactt tttggtgatt atccctaata cccaacagag catgttgctg ctgtgtatta
    26401 gcctggcagt gctgggattc ttcctcggaa tagaagtaca gaaacggcga ctgggctcga
    26461 tgggggcgct ggccagcacc ataaatatta tcgtgctgga taacccgatg actttccatt
    26521 tcagtcagtt tctcgacagc gcattagggc aaatcgtcgg ctgtgtgctc gcgttcaccg
    26581 ttattttgct ggtgcgggat aaatcgcgcg acaggactgg acgtgtactg cttaatcagt
    26641 ttgtttctgc cgctgtttcc gcgatgacta ccaatgtggc acgtcgtaaa gagaaccacc
    26701 tcccggcact ttatcagcag ctgtttttgc tgatgaataa gttcccaggg gatttgccga
    26761 aatttcgcct ggcgctgacg atgattatcg cccaccagcg cctgcgtgat gcgccgatcc
    26821 cggttaacga ggatttatcg gcgtttcacc gacaaatgcg ccgcacagca gaccatgtga
    26881 tatctgcccg tagcgatgat aaacgtcgtc ggtactttgg tcagttgctg gaagaactgg
    26941 aaatctacca ggaaaagcta tgcatctggc aagcgccacc gcaggtgacg gaaccggttc
    27001 atcggctggc ggggatgctc cataagtatc aacatgcgtt gaccgatagt taagtcaaaa
    27061 ccgacgccaa aagcgtcggt tttttcatgg ctatacttag cgatgcacgg cagaactcgc
    27121 cgcgaaacgt gacggtggca acagatgaat atttatacct ttgattttga tgagattgag
    27181 agtcaggagg atttttatcg tgactttagc caaacctttg gtctggcgaa agataaggta
    27241 cgcgatctcg actcactatg ggatgtgtta atgaacgatg tcctgccgct accacttgag
    27301 attgaatttg ttcatctggg agagaaaacg cgtcgccgtt ttggcgcgtt aatattgctg
    27361 tttgatgagg cagaggaaga gctggaaggg catttgcgtt ttaatgttcg tcattagcgc
    27421 aaaaaaaagc ccccgaaccg ggggcaatat cgtcggacaa gacgatgagg gtttatttgt
    27481 acagttcagc cgtagcgtgc caggtgtcac cgctacgagc ttcagtaatc tggtaggccg
    27541 ttgcgccttt ctcttccgct tttttgttca gcatttcacg catatccatt ggcgaagacg
    27601 ccacaccact tacggatacg gtcccgattg cttcacgatt ttgtgcttgt gcagcatcaa
    27661 tggagtcggc agcgaatgca ccgaaagaga gaacagaaag tacgcttaat gcagcaacag
    27721 tggttttgat tttcatgatt tttacctcga cataatcttt tagctgggcc tttgtttcgt
    27781 gacccat
//
LOCUS       DAZKFZ010000045        27710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000045 DAZKFZ010000000
VERSION     DAZKFZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..233)
                     /locus_tag="ACHOQW_001761"
     CDS             complement(<1..233)
                     /locus_tag="ACHOQW_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9572848.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWS"
     gene            complement(459..1784)
                     /gene="rimO"
                     /locus_tag="ACHOQW_001762"
     CDS             complement(459..1784)
                     /gene="rimO"
                     /locus_tag="ACHOQW_001762"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP9572849.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            1997..2380
                     /gene="bssR"
                     /locus_tag="ACHOQW_001763"
     CDS             1997..2380
                     /gene="bssR"
                     /locus_tag="ACHOQW_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP9572850.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2491..3606
                     /gene="yliI"
                     /locus_tag="ACHOQW_001764"
     CDS             2491..3606
                     /gene="yliI"
                     /locus_tag="ACHOQW_001764"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP9572851.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3603..4229)
                     /gene="gstB"
                     /locus_tag="ACHOQW_001765"
     CDS             complement(3603..4229)
                     /gene="gstB"
                     /locus_tag="ACHOQW_001765"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP9572852.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            4475..5677
                     /gene="dacC"
                     /locus_tag="ACHOQW_001766"
     CDS             4475..5677
                     /gene="dacC"
                     /locus_tag="ACHOQW_001766"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9572853.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(5724..6482)
                     /gene="deoR"
                     /locus_tag="ACHOQW_001767"
     CDS             complement(5724..6482)
                     /gene="deoR"
                     /locus_tag="ACHOQW_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP9572854.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(6540..7136)
                     /gene="ybjG"
                     /locus_tag="ACHOQW_001768"
     CDS             complement(6540..7136)
                     /gene="ybjG"
                     /locus_tag="ACHOQW_001768"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9572855.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7421..8658
                     /gene="mdfA"
                     /locus_tag="ACHOQW_001769"
                     /pseudo
     CDS             7421..8658
                     /gene="mdfA"
                     /locus_tag="ACHOQW_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(8699..8983)
                     /gene="ybjH"
                     /locus_tag="ACHOQW_001770"
     CDS             complement(8699..8983)
                     /gene="ybjH"
                     /locus_tag="ACHOQW_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP9572856.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(9069..9884)
                     /gene="ybjI"
                     /locus_tag="ACHOQW_001771"
     CDS             complement(9069..9884)
                     /gene="ybjI"
                     /locus_tag="ACHOQW_001771"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP9572857.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(9884..11092)
                     /locus_tag="ACHOQW_001772"
     CDS             complement(9884..11092)
                     /locus_tag="ACHOQW_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572858.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSMVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            11176..11712
                     /gene="rcdA"
                     /locus_tag="ACHOQW_001773"
     CDS             11176..11712
                     /gene="rcdA"
                     /locus_tag="ACHOQW_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP9572859.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(11887..13572)
                     /locus_tag="ACHOQW_001774"
     CDS             complement(11887..13572)
                     /locus_tag="ACHOQW_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP9572860.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            13842..14219
                     /locus_tag="ACHOQW_001775"
     CDS             13842..14219
                     /locus_tag="ACHOQW_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP9572861.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(14249..14506)
                     /gene="grxA"
                     /locus_tag="ACHOQW_001776"
     CDS             complement(14249..14506)
                     /gene="grxA"
                     /locus_tag="ACHOQW_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP9572862.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            14666..14953
                     /locus_tag="ACHOQW_001777"
     CDS             14666..14953
                     /locus_tag="ACHOQW_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP9572863.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14937..15659
                     /gene="nfsA"
                     /locus_tag="ACHOQW_001778"
     CDS             14937..15659
                     /gene="nfsA"
                     /locus_tag="ACHOQW_001778"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP9572864.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            15720..16622
                     /gene="rimK"
                     /locus_tag="ACHOQW_001779"
     CDS             15720..16622
                     /gene="rimK"
                     /locus_tag="ACHOQW_001779"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP9572865.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            16710..17186
                     /locus_tag="ACHOQW_001780"
     CDS             16710..17186
                     /locus_tag="ACHOQW_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP9572866.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            17537..18649
                     /gene="potF"
                     /locus_tag="ACHOQW_001781"
     CDS             17537..18649
                     /gene="potF"
                     /locus_tag="ACHOQW_001781"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP9572867.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            18744..19877
                     /gene="potG"
                     /locus_tag="ACHOQW_001782"
     CDS             18744..19877
                     /gene="potG"
                     /locus_tag="ACHOQW_001782"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP9572868.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            19887..20840
                     /gene="potH"
                     /locus_tag="ACHOQW_001783"
     CDS             19887..20840
                     /gene="potH"
                     /locus_tag="ACHOQW_001783"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP9572869.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            20837..21682
                     /gene="potI"
                     /locus_tag="ACHOQW_001784"
     CDS             20837..21682
                     /gene="potI"
                     /locus_tag="ACHOQW_001784"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP9572870.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            21742..22230
                     /locus_tag="ACHOQW_001785"
     CDS             21742..22230
                     /locus_tag="ACHOQW_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP9572871.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            22271..23398
                     /gene="rlmC"
                     /locus_tag="ACHOQW_001786"
     CDS             22271..23398
                     /gene="rlmC"
                     /locus_tag="ACHOQW_001786"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP9572872.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            23678..25021
                     /locus_tag="ACHOQW_001787"
     CDS             23678..25021
                     /locus_tag="ACHOQW_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9572873.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDGGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            25043..25363
                     /locus_tag="ACHOQW_001788"
     CDS             25043..25363
                     /locus_tag="ACHOQW_001788"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9572874.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            25375..26856
                     /locus_tag="ACHOQW_001789"
     CDS             25375..26856
                     /locus_tag="ACHOQW_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP9572875.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(26907..27638)
                     /gene="artJ"
                     /locus_tag="ACHOQW_001790"
     CDS             complement(26907..27638)
                     /gene="artJ"
                     /locus_tag="ACHOQW_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP9572876.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
BASE COUNT         6728 a         6692 c         7211 g         7079 t
ORIGIN      
        1 ttggaccaac catcataaaa aaacacatga tttgctgatt taaaaaagat aattgttctg
       61 gctccaccac tctttccttg ttggaggggt aaccgttttt tgatgacacc gctacctaaa
      121 tcagcctccc atatgccttg catcacttct tcagcggcag ccatgagaac tttatcgtga
      181 ataccaagtt ttttcgtgct ttggctgaat cgccttgcct tatacacacc catacatcca
      241 tccttgtggt aatgtttact atagtaacca ttacgcttat ttcaagtaat tgcgatcaaa
      301 actttaattt ggtgtgaaaa agtgagagtt gcctcgagta cgattcattt ttctcgattt
      361 atgcgtcagg ggattttgtg cgtaagggta ggacatcggg aagggtagta gaggcagatc
      421 gaagaacaat atccttgtta gatctgcctt tagattaatt aaacccggct accccacaaa
      481 tcgtactcat cggcgtgctc gactttcaca cgcaggatat cacccggctt aacgttggtt
      541 tcaccattga gataaaccgc accgtcgatt tccggtgcat ctgccatgct gcgaccaatc
      601 gcgccttctt cgtccacttc gtcgataatc accagaattt cacggcccac tttctcttgc
      661 aggcgctcgg cggaaatctg ctgctgcaac tgcatgaaac ggttccagcg ttcttctttc
      721 acttcttccg gaacctggtc aggcagggca ttggcgtctg caccttcaac cgggctgtat
      781 ttaaagcagc caacgcgatc cagacgcgct tctttcagga agtcgagtag catctggaaa
      841 tcttcttccg tctcgccagg gaagccgaca ataaaggttg agcgtagggt cagttccggg
      901 cagatttcgc gccactgttt gatgcgcgcc agttggcgat ctacagaacc cggacgcttc
      961 atcagtttga gaatgcgcgg gctggcgtgc tgcaacggaa tgtccagata cggcaggatt
     1021 ttgccttctg ccatcagtgg gatgacgtcg tccacatgcg gataagggta aacgtagtgc
     1081 agacgtgtcc agatccccag tttcgataac tgttcgcaca ggctgaccat gctggttttt
     1141 accggctcgc cgttgtggaa gccagtacga tgtttaacat caacgccata ggcggaagta
     1201 tcctgcgaga tcaccagaat ctctttaacg cccgcatcta ccagacgttt cgcttcactt
     1261 aatacttcgc caatcggacg gctcaccagg tcgccgcgca tagacggaat aatgcagaag
     1321 gtgcagcggt gattacagcc ttcagaaatt ttcagatagg cataatgacg cggcgtcagt
     1381 ttgacacctt gttctggcac caggctcagg aatgggttgt gtttcggttt tggcacgtag
     1441 tgatgaacgt gctccagaac ctgctcatag ctatgaggcc cggtgatttc cagcactttc
     1501 gggtggactt cgcggatctg atcttctttt gcccccagac aaccggtcac aattaccttg
     1561 ccgttttcat tcaacgcttc accaatggct tccagtgatt cttgtaccgc gctgtcaata
     1621 aagccgcagg tgttgacgat caccatgtcc gcatcgtcat agctcggtac cacgtcataa
     1681 ccttcagtgc ggagttcggt gagaatacgc tctgaatcga caaggttttt cggacagcca
     1741 agggaaacaa agccgatttt cggctgggga gttactttgc tcatagctta aaaaatattc
     1801 agttacagga aaggtcaggg cagggattct acagagttct ggataaaatt tgtatcgcaa
     1861 tctcattcgc tggcggaggc gaaggaaatg taaattttgt taattcggcg tgaagaattg
     1921 atcctggaca gcattatgct caaaaaatag ccatactatt taattgcaac aaggctggaa
     1981 agaggaggat cgaagtatgt tcgttgacag acagcgaatc gatctgctga accggttgat
     2041 cgacgcacgc gttgacctcg ccgcatacgt gcaactgagg aaggcaaaag gatacatgtc
     2101 cgtcagcgaa agcaatcatc tacgagataa cttttttaaa ctgaatcgcg aactgcacga
     2161 taaatcgctg cggttgaatc ttcatctgga tcaggaagag tggagtgctc ttcatcatgc
     2221 tgaagaagca ttagcgacag ccgcagtatg tttgatgagt gggcaccatg attgcccgac
     2281 tgttattacc gtcaacgccg ataagcttga aaattgtctg atgagcttaa cgctgagtat
     2341 ccagagcctg cagaagcacg ccatgcttga gaaggcctga aaactaaggg ggagaaagcg
     2401 tctccccctt catgtttaag tttttgtaaa aatgaatttg ttatctcctc cactgactac
     2461 gctttaagcc agagtcaatc cggaggcgtt atgcatcgac aattcttttt ccttgtgccc
     2521 cttatttgtc tttcttccgc tctctgggcg gctcctgcaa cggtaaatgt cgaagtactg
     2581 caagacaaac tcgaccatcc ctgggcactg gcctttttac ccgataacca cggtatgtta
     2641 atcactctgc gcggcggcga gttgcgtcac tggcaagcag gaaaaggatt atctgcgccg
     2701 ctttccggag ttccggacgt ttgggcgcac gggcagggcg gcctgctgga cgtggtttta
     2761 gcgcctgatt ttgctcagtc tcgccgcatc tggttaagtt attccgaagt tggcgatgat
     2821 ggcaaagccg gaactgctgt ggggtatggc cgcttaagtg atgatctctc aaaagtgacc
     2881 gactttcgca ccgtctttcg ccagatgcca aaactgtcta ccggcaacca ttttggcggg
     2941 cggctggtat tcgacggtaa aggttatctt tttattgctc tgggcgaaaa caatcagcgc
     3001 ccgacggcgc aggatctgga taaattacag ggcaaactgg tgcgtctgac cgaccagggc
     3061 gaaatcccgg atgataatcc ttttataaag gaatccggtg cgcgcgccga gatctggtct
     3121 tatggcattc gtaatccgca aggaatggcg atgaatccgt ggagtaatgc actgtggctg
     3181 aatgaacatg gcccgcgcgg tggtgatgaa attaatatcc cgcaaaaagg caaaaactac
     3241 ggctggccgc tggcaacctg gggaatcaac tattcaggct ttaagatacc ggaagcgaaa
     3301 ggggagatcg tcgccgggac cgagcaacct gttttttact ggaaagattc gcccgccgtg
     3361 agcggcatgg ccttctataa cagcgataaa ttcccccagt ggcagcaaaa attatttatt
     3421 ggcgcgctga aagataaaga tgtcattgtg atgagcgtca acggcgacaa agtaacagaa
     3481 gatggccgta ttttaacgga cagagggcag cgaattcgtg atgttcgcac tggacccgac
     3541 ggttatttat acgttctcac cgacgagtcc agtggggaat tacttaaagt tagcccacgc
     3601 aattagctaa cgggaatcat caccactttg cgcactgcgg ggcgttcagt gagttgctga
     3661 taccagcgtt gcagatttgg acgcggtgtc caggtcaggc caacattaaa caaattgtag
     3721 atgaacggtg cgatagcgat atcgcccaca ccaaactcgt cgccagagaa ccattttact
     3781 tttgccagtt ccgcatcgag cagggcaaac agggcgtcgc actctttgca actggcatca
     3841 atggcggcct gatcgcgctc ttccggtggt gttctgacta atcccatcag gatcccgcga
     3901 tgagcattac tgagtgtctg gtttgcccag tccatccatt tttctgcttc cgcacgacgt
     3961 gccggtgagt cgatccacag gcgtttttgc ccgtactgtg ccgccagata gcggacaatg
     4021 gcgtttgatt cccaaagaat gagatcactt tcgtcgtcac gcaacaacgg caccagcccg
     4081 ttagggttca tcgccagaaa atcagcatcg tgatttatcc caaactcacg gcccgcgaga
     4141 atttgctcat aaggtagttc cagttcttcg agcgtcagca atactttttt tacgttggtt
     4201 gaattattcc gaccccacag cgtaatcata ctcacccctt ttccgacagg cagccacagg
     4261 ctgaacagga ctccgatggt gaagtaaagt taacttttaa gcaacagctg gcaaaaaaat
     4321 gacaccagaa gcagcgaagc ggcaggatat tgcataaact ttaaaaactt taccaactta
     4381 cggtttcttt aagtttgtgt gtgcgttatt aatcaccgaa cttatcatac ggcgatataa
     4441 cgtatttttt tgaatggata ctcgggtggc atttatgacg caatactcct ctctccttcg
     4501 tggtcttgca gcgggttctg catttttatt cctttttgcc ccaacggcat tcgcggccga
     4561 acaaaccgtt gaagcgccga gcgtggatgc gcgtgcatgg attttaatgg attacgccag
     4621 cggtaaagtg ctggcagaag gcaatgcgga tgagaaactg gatcccgcga gcctgactaa
     4681 aatcatgacc agctatgtgg ttgggcaggc gcttaaggcc gataagatta aactcaccga
     4741 tatggtgacg gtcggtaaag atgcctgggc gacgggaaat ccggcactgc gtggttcatc
     4801 ggtaatgttc ctcaaaccgg gcgatcaggt ttcggtggca gacttgaaca aaggtgtgat
     4861 tatccagtcc ggtaatgacg cctgtattgc gctggccgat tacgttgccg ggagccagga
     4921 gtcatttatt ggtttgatga atggttatgc caaaaaactg gggctgacca acactacctt
     4981 ccagacggtg cacggtctgg atgcgccggg gcagttcagt accgcgcgcg atatggcatt
     5041 gctgggtaaa gcattgatcc acgatgtgcc ggaagagtac gccattcata aagagaaaga
     5101 gttcaccttc aacaaaattc gtcagcctaa ccgtaaccgt ctgctgtgga gcagcaatct
     5161 gaatgttgat ggcatgaaga caggaaccac ggcaggcgcg ggatataatc tggttgcttc
     5221 ggctacccag ggcgatatgc gtttaatctc cgtagtgctg ggggcgaaaa ccgaccgtat
     5281 ccgttttaat gagtctgaga aattattgac ctggggtttc cgcttctttg aaaccgtgac
     5341 gccaattaaa cctgatgcca cctttgtgac tcagcgcgtc tggtttggtg ataagagcga
     5401 agtgaatctc ggggcaggcg aagcgggctc agtgaccata ccgcgtgggc agctgaaaaa
     5461 cctgaaggcg agctacacgt taacggaacc gcagcttacc gcaccgctga aaaaaggtca
     5521 ggttgtcggg accattgatt tccagcttaa cggtaaatcc attgagcagc gtccgctgat
     5581 cgtgatggaa aatgtggaag agggcggatt ctttggtcgg atgtgggatt tcgtgatgat
     5641 gaaattccat cagtggttcg gcagctggtt ctcttaatct tctgataacc ggatggcgcg
     5701 aaacgtcatc cggttatacg tcattaatac atcaacttaa tacgctgcgt ctgcgcgtac
     5761 ttcacatact catcttccgg gcaacaatcg ctcaccacaa tatcaaagcg tttcaggtca
     5821 cccatgcgcg ccggacgcac cttgccaaat ttactgtggt caacaaccag cacatgcttt
     5881 tgcgccatcg acatggccca gtgttttacc ggcaactctt caagattaaa acaggtagcg
     5941 cctttactga catgcacgcc cgccgcagaa taaaaagcga tatccgggca aaaattattc
     6001 agcgtttgct gaaaatcgat gggtttgaaa atggcgttgc tggcgtgaaa ttcaccaccg
     6061 cacagaaatg cgcggcaatg gggtttttct ttcagcgcca gaaaggtatt tagcgaataa
     6121 caaacggcgg taaaagggat ttcattatca atcgcttcaa taatccacgg cgtggtggtg
     6181 ccacagtcaa aaaagagggt ctgatcgggt tctaccagtg tcgcagccag ttttgccgcc
     6241 cggcgttttt cttccaccag gcgggatttt tgatcgctta acaggtaatg gctggcgctg
     6301 cgcggttcca gaacaatata gccgccgagc aaaacgacgg gcgcactgtg gttgttcaga
     6361 tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg cggcgtcttt aagatgtaac
     6421 ttatcgctgc gttttaattc ttgcagcagc tgcccgatac gctcttcgcg acgtgtttcc
     6481 ataatccctc tgaatagtta ttgaagcgag ccgctcaata ctacactttt tagcagagat
     6541 cagtcacgca cccagccttt gcggatcggt aatgcaaaac agaagcgata ccacgattgc
     6601 agacgttgat agagtttatg ccccatcgct tgccagataa tctgggcact aaggcagcca
     6661 atcatacctg ccagcaatcc accgagcata tccagcggcc agtggacgcc aagataaacg
     6721 cgcgaccagg caatgacgac ggccagcacc attaaaagtg agccggacca caggcgatgc
     6781 cagcataaaa atgccagtgc aaaggtgaaa atcaccgtac cgtgatcgct cgggaatgag
     6841 tcatccgccg catgatgcag gaagttatag ccgatatttt cgacaaacgg tcggtcgtgc
     6901 ggaaaaagat gtcccatcgt ccaggacacc aacaggctga ccgccagcgc gatagcgatt
     6961 tttatcacca gttgccgttg tgctgtaagc ccccacaacc aaagtaccgc ggccagcagc
     7021 ggcaccacgg taatcaaatc tttagcaata aaaatcgcca acgagatcat ccacggagcc
     7081 gagtctggcg tcgcgttaat aagagagaat agagagagat tcaaattttc cagcataact
     7141 tcccgacgca aagtgattaa aagggagcca atacaggcaa gtcgttgaga ataaagtgca
     7201 ggttaaactg ggtaaagcgg catcgtctta tttccctcaa gcggcctgtt tacggtgggt
     7261 gattgtaacg ggcataggtt aaataaaact taaagaaagc gtagctatac tcgcaataat
     7321 gtaagaatgt gcttaaccgt ggtttcagct acaaaattcg ctttctcgtt agctgcgctt
     7381 ttattaaact ctgcgcgatt attattggcg aagaaattgc atgcaaaata aattagcttc
     7441 cggtgccagg cttggacgtc aggcgttact tttccctctc tgtctggtgc tttacgaatt
     7501 ttcaacctat atcggcaacg atatgattca acccggtatg ttggccgtgg tggaacaata
     7561 tcaggcgggc attgattggg ttcctacttc gatgaccgcg tatctggcgg gcgggatgtt
     7621 tttacaatgg ctcctggggc cgttgtcgga tcgtattggt cgccgtccgg tgatgctggc
     7681 gggtgtggtg tggtgtggtt tattgtcacc tgtctggcaa tattactggc gcaaaacatt
     7741 gaacaattca ccctgttgcg cttcttgcag ggcataagcc tctgtttcat tggcgctgtg
     7801 ggatacgccg caattcagga atccttcgaa gaggcggttt gtatcaagat caccgcgctg
     7861 atggcgaacg tggcgctgat tgctccgcta cttggtccac tggtgggcgc ggcgtggatc
     7921 catgtgctgc cctgggaagg gatgtttgtc ttgtttgccg cattggcagc gatctccttt
     7981 ttcggtctgc aactagccat gcctgaaacc gccacgcgta taggcgagaa actgtcgctg
     8041 aaagaactcg gtcgtgacta taagctggtg ctgaagaacg gtcgctttgt ggcgggggcg
     8101 ctggcgctgg gattcgttag cctgccgttg ctggcgtgga tcgctcagtc gccgattatc
     8161 atcattaccg gcgagcagtt gagcagctat gaatatggtt tgctgcaagt gcctattttc
     8221 ggggcgttaa ttgcgggtaa cttgctgtta gcgcgtctga cctcgcgccg caccgtacgt
     8281 tcgctgatta ttatgggcgg ctggccgatt atgattggtc tgttggtcgc tgctgcggca
     8341 acggttatct catcgcacgc gtatttatgg atgaccgccg ggttaagtat ttatgctttc
     8401 ggtattggtc tggcgaatgc gggactggtg cgattaaccc tgtttgccag cgatatgagt
     8461 aaaggtacgg tttctgcggc gatgggaatg ctgcaaatgc tgatctttac cgtcggtatt
     8521 gaaatcagca aacatgcctg gctgaacggg ggcaacggac tgtttaatct cttcaacctt
     8581 gtcaacggaa ttttgtggct gtcgctgatg gttatctttt taaaagataa acagatggga
     8641 aattctcacg aagggtaaaa aaatgcctga ttgctttgtg cgatcaggca ttctcgaatt
     8701 aatggtgatg gtcgtcaatc tggtgttcga taaccatccc ttcacctacg ctggcaagat
     8761 ggcgaacata aggatgcggg tggtaagccg gagctggcgc aggagccaca tagacggttt
     8821 gcggcacagt cgcgacgctg accgcttgtg gaacgctgac tgaatcagga accacaacca
     8881 ctttgtatcc actcggcaca tcgacggtga cactttgcgc catcgccaca ccagtaaagc
     8941 ccattaaaag cgcgcccaac agtagacaat ttttcataat catttcgcct gaaataatgc
     9001 agcgggttaa tttccgcgaa ttatgcagcc ttcattcagg tattgtgttg ccgggcgtgc
     9061 tgccgttttt attggtcaaa tggcgcttcg tgtttaagaa ctttatcgat cacatccagt
     9121 acgccttcac ggttattgga gcctgcccgg tattttgccg ctgcgacgac cgcgttgccg
     9181 gcattttcca ttgcaaaact aaagcctgcc tgacgcagca tctcaatatc gttaccgcca
     9241 tcgccaaaga ccaccacttc gctgtcgtct attccccata atttctgcag ttggcgaagg
     9301 ccattggctt tatgtacgcc ggggataatc agatcgatgc tgccgttgcc ggtgtggacc
     9361 ggcaccataa tatcgccgat ggcctcatgt aatgtttttt gtacttgtgg aatcagttca
     9421 tcggaaagat tcaggccaaa cttaaagaag atatcttcta agttgtcaaa gttatcgacg
     9481 tattccagac ggtgataata catttccgcc accattttca tggcatcgtc atattttttg
     9541 agcgtatagg cgctattttt tccgcaggca ataatttcca cttccgggcg cgtcagcaaa
     9601 tgttccacga cagtagtaaa cgcatccttc gacagctcgc cattaaaaac atctttgcct
     9661 tcactcacta cccagccgcc gttttcagcc acaaaggcaa tttcattagc aatttcaggg
     9721 aagaaagaga tcaactgata atattgattc ccgctggcga ccacaaagcg aatgccttgt
     9781 gctttcattt gctgatactg agccataaac cgctcacggt tataggtttt ttgatcgctt
     9841 aagaaagtac catccatgtc taccgcaatt aatttaatgc tcatcaacta ttctccatcg
     9901 ccgtctgcgt tttggtatcg ggtttggcga cggctttcgc gacaatagcc gcgagaataa
     9961 ccagcgccag tacaaccagc attgcactac gtaatccata atgttcgccg agatagccca
    10021 gcagcggcgg cccgacgagg aaagccagat aaccggtcgt tgctaccata ctgacgcggg
    10081 ttggtgcatc ggggccggta tcgctggcgg cagaaatggt cagcgggaag cccagcgagg
    10141 cacccagtcc ccagagtaca acagacaccc cagcgaccca ggcgctatcg acaaaaataa
    10201 tcagcccaat acccaacgcc cccattagcg cactggcccg aaccacggca acgcgactgt
    10261 aacggtcgat gaaccaaccg ccagtgaagc gtccaacggt catccccagg gtaaaaccgg
    10321 cataaatcag cgagccggaa gtggggctaa aaccgtgacc gtcaaccatt aataagggta
    10381 accagtcgtt ggcagaacct tcggcaaagg ccatcgccag caccacaaca ccaatcagca
    10441 gcaactggat atcgcgataa aaaggtacgc ctttttcgcc atgctgggtg ccatcggcag
    10501 catttttgcc cgtaccgtca gggattgcct gaatggcgat atatataggc gcgatgccta
    10561 ccagcgccgc caataaaatg tgcaccgttg ccggaacgcc aaaggccgtc agtgccatcc
    10621 cgacaccagc gcctgccagc gtgcccaggc tataaaaacc gtgcatcatc ggcaaaaccg
    10681 ttttattcat ttctcgctca acggcggcac cttcaacgtt tatcgccact tccgcagaac
    10741 caaaacttgc cccaaagacg ccgagaccaa cggcaaagag caggggcgat gtcagccaga
    10801 gtgccagact taatatcatc atcccgacca atgcgcagga catggtgacc aggatgacat
    10861 tacgtgtccc aaagcgtttc actaaccacg ccgagcagag aataccgctc atcgaaccga
    10921 tcgacagacc aaagagaaca ccgcccattt cagcgatcga gacagagaga atatcgcgga
    10981 tagcaggcgt acgggttgcc caggacgcca ttaacaggcc cggcaaaaag aagaacataa
    11041 acagcgccca ggttcggcgt ttcaatgcat tacgtgaaga atttacggtc atagatcacg
    11101 tcaaaataag aagagggaag acaacattag caaggttgtg tacatttgta cacaattgca
    11161 gacagaggaa atgacatgcg tcgcgctaac gatccgcaac ggcgagaaaa aattatccag
    11221 gccacgctgg aggcggtgaa actttacgga atacatgctg ttacgcaccg caaaattgct
    11281 acccttgccg gggtaccgtt gggatcgatg acctactatt tttcaggaat tgatgagttg
    11341 ttgctggagg cgttcagcag ttttactgag atcatgtccc ggcaatatca ggcatttttt
    11401 agcgatgtta gtgatgctca aggcgcatgc caggctatca ccgatatgat ctacagctca
    11461 caggttgcaa cgccggataa catggagctg atgtaccagc tctacgcgct ggctagccga
    11521 aaaccgctat taaaaacggt aatgcaaaac tggatgcagc gcagtcagca aacgctcgaa
    11581 caatggtttg aacccggaac cgcccgcgcg cttgatgcgt ttattgaggg gatgacgctg
    11641 cattttgtca ccgaccgtaa gccgctatcg cgcgaggaga ttttgaggat ggttgagagg
    11701 gttgcagggt agtagataaa tttcaggcaa caaaaaaccc atcaaccttg aaccgaaatg
    11761 gcggggttga tgggctccac aaaatgggga catcaaagaa aagcagtggc actaattaag
    11821 actgatgccc tgcggaaaag ttctgcggtt gtgcaaaaaa atttcatttt cagggcaact
    11881 tcagttttat cctaatcctg gccataccat gacgatgatt gtccctgcca gcgtcagcag
    11941 gacgttggcg attgcgtagg tgcccgcata gcccagcgcc gggatgttac tgcgagctgt
    12001 atcactgatg atctccattg ccggcgcgca ggtgcgtgcg cccatcattg cgccgaacaa
    12061 cagtgcgcgg ttcattcgca atacataagc accgaacaag aaacagataa ccacgggcac
    12121 aagactgaca atcaatccgg caatcaacat ctgaccgcca atcgcgccca gaccgttatt
    12181 aataccgcta ccggcgctca gaccaacgcc tgccataaac accatcaagc cgaactcttt
    12241 caccatgctt aatgcccctt gcggaatgta accgaaggtc gggtggttag cacgcataaa
    12301 gcccagcata attccggcga ataacaaccc ggcagcgttc cccatgccga aactgaatgt
    12361 gctgaactgg aaggtgatca tcccgatcat cagcccaata acaaagaagg cgcagaatgc
    12421 cagcaggtca gtgacctggc tgtgaatcga gataaagccg atgcgatcgg cgatggtttt
    12481 tacgcggcgg gcatcgccgc tgacttgtaa aacgtcacct ttgttaagca cgacgttgtc
    12541 atcaatcggc atctcaatct ggctacgaat gacgcggtta aggaagcaac cgtgatcggt
    12601 caacttcagt tgtgcgagac gtttacctac agcgttatgg tttttaacga ccacttcttc
    12661 agtgacgata cgcatgtcga gaaggtcacg atcgaaaact tctttaccgt tacggaagct
    12721 gggatcgagt cgggcatggg cgtcgggata gcctaccaac gctatttcat cgcccatttg
    12781 tagcacggca tcaccgtctg gatttgccag aatcccgtta cgtcgaatac gttcaatgta
    12841 gcagccggtt tgtcgataaa tacccagttc acgcagattt ttgccgtcgg tccaggccac
    12901 cagctccggg ccgacgcgat aggcgcggat caccggtaaa taaaccttac ggttggcatc
    12961 agtgtccagg ccacgttcgc gggcgatttg ctgggcgctg gtctgtaagt cctgatgctg
    13021 caatttcggc aagtaacgcg caccaacaat caaactcacc agaccgatta aataggttaa
    13081 ggcatacccg aggctcagat tatccagtgc cagtgagagc tgcctgcttt ccataccgga
    13141 atgacgcagt gtatcgccag caccgaccag aaccggtgtc gacgtcatag agcctgctaa
    13201 cataccggcc gtcaggccaa tatcccagcc aaacagctta cctaacccta aggcgatcac
    13261 cagcgcactg ccaaccatca ccagtgctaa cattaggtaa tttttcccat cgcgaaaaaa
    13321 aatggaaaaa aagttcggtc cggcttcgac cccgacgcag aaaataaaca gcataaagcc
    13381 aagattaagc gcatcggtgt taatgctgaa atgttgttgg cctaataaca gcgatacgac
    13441 taaaacgcca atggaattac ccagttggat cgaaccaagt cgtaactttc cgagacatag
    13501 cccaagcgcg aggaccacaa ataataacag aatgtaattc ccatttaaca attcggcgac
    13561 gtttatattc acggaggcta acttcttgtt tactagtaag ctgttgaaag aaatggtaat
    13621 ttacgataat gttttttacc agaattcagg gcgcagattc attcagcgca cctaaacgat
    13681 agtaaagtaa caatatattt tactagtgta atcacattag gtatcaacgg ctatatgaat
    13741 tgcgttggcc tatattagca tggaatgcga agcggcttta tcttactgaa cgccacactg
    13801 gcgaaaaatg tgttcgatag acgcagtgtc aggaggaacg agtgaaacat aaacaacgtt
    13861 gggcgggggc aatctgctgt tttgtcctct tcattgtggt gtgccttttt ctggcgacgc
    13921 acatgaaagg cgcttttcgg gctgccgggc atcctgaaat cggcttgctg tttttcattc
    13981 ttcctggagc agttgccagt ttcttttcac agcgtagaga agtcctgaaa cctctatttg
    14041 gcgcaatgct ggcggcaccc tgttcaatgc ttattatgcg gctgtttttt tcaccgaccc
    14101 gctcattctg gcaagagctg gcatggttac taagcgcggt gttctggtgt gcgctggggg
    14161 cactgtgttt cttatttatc agtagcttgt ttaaaccaca gcacagaaaa aatcagtaaa
    14221 gccctcaacg cgagggcttg tcagacgatc aggcgtccag attctctttc acccatgcag
    14281 caaaatcggt atagccgccg atatgttgct gatcgacaaa aatctgcggc acggtttcta
    14341 cgggtttacc tgccttttgt tgtagatctt ctttagtgat cccttccgca cgaatatcta
    14401 catactgata ctgaaaatca tcgcgttcat tgctcaattt ctcagccaga tcttttgcac
    14461 gcacacagta agggcaaccc ggacgaccaa aaataacggt ttgcattatt tctctcctca
    14521 tagatttatg cctgtaatga tcacgctaaa atgtattcgc tgaaagtagg tttaacctgt
    14581 tgcattaatt gctaaaagct ataactgtta aacacaatac agtgaaaagt tttagactga
    14641 aggttcactt tgcagaggga agcgtatgcg cgcgatcggt aaattgccta aaagcgtgtt
    14701 gatactggaa tttatcggaa tgatgttgct ggcggtggcg ctgctgtcgg taagcgactc
    14761 cctgtcgctg cctgagccat tttctcggcc agaagtgcag attctgatga tttttctcgg
    14821 tgttttgctc atgcttcccg ctgcggtggt ggttattctt caggtggcaa aacgtcttgc
    14881 cccacagctg atgaaccgtc caccgcagta ttcacgttca gaaagagaaa aagataatga
    14941 cgccaaccat tgaacttact tgtggccatc gctccattcg ccatttcact gatgaaccca
    15001 tttccgaggc gcagcgtgag gcgattatta acagcgcccg tgcgacgtcc agttccagtt
    15061 ttttgcagtg cagtagcatt attcgcatta ccgacaaagc gttacgtgaa gaactggtga
    15121 cgctgaccgg cgggcaaaaa cacgtagcgc aagcggcgga gttctgggtg ttctgtgccg
    15181 actttaaccg ccatttacag atctgtccgg atgctcagct tggcctggcg gaacaattgc
    15241 tgctcggtgt cgttgatacg gcaatgatgg cgcagaatgc attaaccgca gcggaatcgc
    15301 tgggattggg cggggtatat atcggcggcc tgcgcaataa tattgaagcg gtgacgaaac
    15361 tgctgaaatt accgcagcat gttctgccgc tgtttgggct gtgccttggc tggcctgcgg
    15421 ataatccgga tcttaagccg cgtttaccgt cctccatttt ggtgcatgaa aacagctatc
    15481 aaccgctgga taaagacgca ctggcgcagt atgacgaaca actggctgaa tattacctca
    15541 cccgtggcag caataatcgc cgggatacct ggagcgatca tatccgccga acaatcatta
    15601 aagaaagccg cccatttatt ctggattatt tgcacaaaca gggttgggcg acgcgctaaa
    15661 accgccacgc cgatgtatga tacgcgggct tttgaccagg tctgacagag aggtgcaggg
    15721 tgaaaattgc catattgtcc cgggatggaa cgctctattc gtgtaagcgg ctgcgtgaag
    15781 ccgctataca gcgcggtcac ctggttgaaa ttcttgatcc gctttcttgc tacatgaaca
    15841 taaatcctgc ggcgtcttct attcactaca aaggccgcaa gttaccccat tttgacgcag
    15901 tgatcccgcg tattggcacc gccattacct tttatgggac ggcggcactg cgccagttcg
    15961 agatgctggg gagctatccg ctcaatgagt cggtcgccat tgcccgggcg cgtgacaaat
    16021 tgcgttccat gcaactgctg gcgcgtcagg gcatcgacct gcctgtcacg ggcattgcgc
    16081 attcgccgga tgataccagc gatttaatcg acatggtcgg tggtgcgccg ctggtggtca
    16141 agttggttga aggcacgcag ggaattggcg tcgtgctggc ggagacgcgt caggcggcgg
    16201 aaagcgtgat tgacgctttc cgcggtctga acgcgcatat tctggtgcag gaatatatca
    16261 aagaggcgca agggtgcgat atccgctgtc tggttgttgg cgatgaagtg gtcgctgcga
    16321 ttgaacggcg ggcgaaagag ggcgattttc gttccaattt gcatcgtggc ggcgcggcaa
    16381 gcgtcgccag tatcacacca caggagcgtg aaatcgcgat aaaagccgcg cgaacgatgg
    16441 cgctggacgt tgctggtgtg gatattctgc gtgctaatcg cgggccgttg gtgatggaag
    16501 tgaatgcgtc gccggggctg gaaggaatag aaaaaaccac cggtatcgac atcgcgggta
    16561 aaatgatccg ctggatcgaa cgccacgcta cgacagaata ttgcctgaaa acgggtggtt
    16621 agtcgcaatc acattactga tcatggtttt gcctgctctt tttgcgtaag ctgtgccggt
    16681 ctttttatcg aaagaggttg tacaaaatta tgacatcgct ggtcgttcct ggtctggata
    16741 cgctgcgtca atggctcgat gacctgggga tgagtttttt tgaatgtgat aactgtcagg
    16801 ctctgcatct gccccatatg cagaatttcg acggtgtctt tgatgccaaa atcgatctga
    16861 tcgataacac gatcctgttt tctgccatgg cggaagtccg accttcagcc gtattgccgc
    16921 tggcggcgga tttatctgcc atcaatgcca gttcgctgac cgtgaaagca tttcttgata
    16981 tgcaggatga taatctgcca aagctggtgg tttgccagtc tttatccgtt atgcagggcg
    17041 taacctatga gcagtttgca tggttcgtgc gtcagagcga agagcagatt tcgatggtca
    17101 ttcttgaagc taatgcccat caactgctgt taccgactga cgatgaaggg caaaacaacg
    17161 ttaccgaaaa ctatttcctc cactgataac tcctttcgag cacgcagtcg ctggtgcagt
    17221 ggctgcgcgc tgcaaaatta tctgctgttt ttaacctttt cttaaagatt atttcacttc
    17281 tcttgtgtcg atttggcttt atcacataga gcaaatacgc ataaaaattt gttaaatacc
    17341 gttttttaat ccgagctata gtctcaaacc ctggctaaag ttattcttgc gatgctttta
    17401 tatagcgagc agtgctggcc gggagagagt tctcttttct tacaccgcgc cgataaaaaa
    17461 tatgcacgtt tattgcatat ctttcagtgt gacaactttt gttcgtttgt taacgaactt
    17521 tcagaaggaa agagatatga ccgccttaaa taaaaaatgg ctttcgggtc tggttgcggg
    17581 tgctctgatg gccgtctctg tcggcacgct cgcggctgaa caaaaaacac tccacattta
    17641 taactggtct gattatatcg cccccgacac ggtggctaac tttgaaaaag aaaccggtat
    17701 taaagtcgtc tacgatgttt tcgactctaa cgaagtactg gaaggcaaat taatggccgg
    17761 gagcaccggc tttgacctgg tggtgccgtc agcaagcttt cttgaacgcc agctgacagc
    17821 gggagttttc cagccgctgg ataaaagcaa attgccggag tggaagaatc tcgacccgga
    17881 actgctgaag ttggtcgcca aacacgatcc cgacaataaa tttgctatgc cctatatgtg
    17941 ggcgacgacc gggattggct ataacgtcga taaagtcaaa gcggtgctgg gcgaaaacgc
    18001 gcccgtcgat agctgggact tgatcctcaa gcctgaaaat ctggaaaaac tgaaaagctg
    18061 cggtgtatct ttcctcgatg cgccagaaga agtttttgct accgtgctga attatctcgg
    18121 caaagatccc aacagcacta aagcggatga ttacaccgga ccggctaccg atctgctgtt
    18181 aaagctgcgc ccgaacattc gttatttcca ttcatctcaa tatattaacg acctggcaaa
    18241 cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc tggcaggcgt caaaccgcgc
    18301 gaaggaagcg aagaatggcg tgaatgtctc gttctcgatt ccaaaagaag gggcgatggc
    18361 gttctttgat gtattcgcca tgcctgcgga tgccaaaaat aaagacgaag cctatcagtt
    18421 cctgaattac ctgctgcgtc ctgatgtggt ggcacatatc tctgaccatg tgttctatgc
    18481 taacgccaat aaagcagcca cgccgctggt gagtgcggaa gtccgtgata acccgggtat
    18541 ttatccgcct gcggatgtcc gcgcgaagtt gttcactctg aaagtacagg ctccgaaaat
    18601 cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc ggaaaataat ccgcagcctt
    18661 agatgccggg cgggagcgcc actccctccg gcaattcgca ccaacatggt gcgcttgcac
    18721 acatttaatg ccggagagca gccgtgaatg acgctatccc tcgcccccag gcgaaaaccc
    18781 gtaaggcgct gaccccgcta ttagaaatcc gcaatctgac caaatcctac gatggtcagc
    18841 atgcggttga tgatgtcagc ctgaccatct acaaaggtga aatctttgcg ctgctgggcg
    18901 catccggctg tggcaagtcc acgctgctgc gtatgctggc aggtttcgaa caaccttcag
    18961 ccgggcagat tatgcttgat ggcgtcgatt tgtcacaggt gccgccttac ctgcgcccca
    19021 tcaatatgat gtttcagtct tacgcgctgt ttccgcatat gaccgtggaa cagaacatcg
    19081 cttttggcct gaaacaggac aaactaccga aagcggaaat tgccagccgg gtcaatgaga
    19141 tgctcggcct tgtgcatatg caggagttcg ccaaacgcaa accgcatcag ctttccggtg
    19201 gtcagcgaca gcgtgtggca ctggcccgaa gccttgccaa gcgcccgaaa ctattactgc
    19261 tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga ccggatgcag cttgaagtgg
    19321 tggatattct ggaacgcgtc ggtgtgactt gtgtgatggt cacccacgat caggaagagg
    19381 cgatgaccat ggcggggcgt atcgccatta tgaatcgtgg gaaatttgtc cagattggcg
    19441 aaccggaaga gatctacgag catccgacta cccgctatag cgccgaattt attggctcgg
    19501 ttaatgtctt tgaaggtgta ctcaaagagc gtcaggaaga tggtctggtg ctttattcgc
    19561 cggggctggt gcatccactg aaagtcgatg cggatgcttc agtggtcgat aacgtgccgg
    19621 tacacgtagc gctgcgtccg gaaaaaatca tgctttgcga agagccgccc gccaatggtt
    19681 gtaacttcgc ggtgggggag gtgatacaca ttgcctatct cggcgatctt tcggtgtatc
    19741 acgttcgtct gaaaagtggg cagatgatta gcgcccagct acaaaacgcc catcgtcatc
    19801 gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg ctgggaagtg gacagctgtg
    19861 tggtgctgac ggtttaagga gcaaagatga gtacacttga acctgctgcc cagtcgaaac
    19921 cgccgggcgg atttaagctg tggttgtcgc agttgcaaat gaagcatggg cgcaaactgg
    19981 tcattgcgtt gccgtatatc tggttgatct tgctgtttct gctgccattt ctgattgtct
    20041 ttaaaataag cctggcggag atggcgcgcg ctattccacc ttataccgaa ctgatggagt
    20101 gggctgacgg gcaactgtcc atcaccctta atttcggtaa tttcctgcaa ctgaccggcg
    20161 atccgctcta tttcgatgct tatctccagt cgttacaggt ggcggcgatt tcgacatttt
    20221 gctgtttact gatcggctat ccgctggcgt gggcggtggc gcacagtaag ccttcgaccc
    20281 gtaatatctt attactactg gtgatcctgc cgtcgtggac ctcgtttctg attcgtgtct
    20341 acgcctggat gggaatatta aaaaacaacg gtgtgctgaa taattttctg ctgtggctgg
    20401 gggttatcga tcaaccgctg accattcttc ataccaatct ggcagtttat attggcattg
    20461 tttacgccta cgtgccgttt atggtgctgc cgatttatac cgcgttgatt cgtattgatt
    20521 attcgctggt ggaagcagcg ctggatctcg gtgcacgacc gctgaaaacg ttctttactg
    20581 tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc gatgctggtg tttatcccgg
    20641 ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc ggacagcatc atgatcgggc
    20701 gcgtgctatg gcaagagttt ttcaataacc gcgactggcc ggtggcctcg gcggtagcga
    20761 tcatcatgtt gctgctgcta attgtgccga taatgtggtt tcacaaacac cagcaaaaaa
    20821 gcgtgggaga acacggatga ataatttacc ggtagttcgt tcgccctggc ggattgtgat
    20881 tttgctgttg ggcttcacct ttctctacgc gccaatgctg atgctggtta tctattcgtt
    20941 taacagctcg aagctggtga cggtgtgggc cggctggtca acgcgctggt atggcgaatt
    21001 attgcgcgat gacgcgatga tgagtgcggt tggtttaagt ctgacaattg cggcctgtgc
    21061 ggcgacggcg gcggcgatcc tcggaactat tgcggcggtg gtgctggtgc gctttggcag
    21121 gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg ccgctggtaa tgccagatgt
    21181 catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt gctcatgcca ttggctggcc
    21241 tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc actttctgta cggcttatgt
    21301 ggcagtcgtt atttcgtcgc gtcttcggga actggatcgc tcgatagaag aagcagcgat
    21361 ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt acgctaccga tgatcatgcc
    21421 cgcggtcatt tctggctggt tactggcttt tactttgtcg cttgatgatc tggtgatcgc
    21481 cagctttgta tccgggccgg gagccaccac gttaccgatg ctggtctttt ccagcgtgcg
    21541 gatgggggtg aatccggaaa tcaacgccct agcaacattg atcctcggcg cggtcggaat
    21601 tgtcggattt atcgcctggt atctgatggc tcgcgcagaa aaacagcgga tacgcgacat
    21661 ccagtgtgca agacgtggct gaagacacta aaatttgcca acctggctac ataatgccgc
    21721 gtatgtcgcg gcattgtttt catggaagac gaaacgttgg gattttttaa gaaaacatct
    21781 tcatctcatg ctcgcctgaa tgtgcctgcg cttgtgcagg tggcggcgct cgccattatt
    21841 atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc tgggcgtgcg cggtattggc
    21901 gagttcattc atcgcagcgt acaaacctgg agtttaacgc tggtcttttt aagcagtctg
    21961 gtgctggttt tcattgagat ctggtgtgcg ttttcactgg tgaaagggcg tcgctgggcg
    22021 cgctggctgt atctgctgac acaaatcact gccgcaagtt acttgtgggc ggcttcgctg
    22081 gggtacggtt atccggagct gttcagcatt cccggtgaat caaaacgtga aatcttccat
    22141 agcctgatgc tgcagaagct gccggatatg ctcatcctga tgctgctgtt cgttccctcg
    22201 accagtcggc ggttcttcca gttgcaataa tgtgtataat cgtcgcccct gatgatgtga
    22261 aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc tgtcgttcct gtcagtggat
    22321 aacgcagccg attccagagc aactctccgc taaaaccgcc gatcttaaaa atctgctcgc
    22381 cgactttccg gttgaggaat ggtgcgcgcc ggtgtcaggc ccggaacaag ggtttcgtaa
    22441 taaagccaaa atggtggtga gtggtagcgt tgaaaaacca ctgctcggta tgctgcatcg
    22501 cgacggcaca ccggaagacc tttgtgactg cccgctttat cctgcctcat ttgcgcccgt
    22561 ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta acgccctaca acgtggcgcg
    22621 taagcgtggc gaactgaaat acattctgct gactgaaagc cagagcgatg gcggcatgat
    22681 gctgcgcttt gtactgcgtt ctgaaaccaa actagcacaa ctgcgtaagg cattgccgtg
    22741 gttacaggca caattgccgc agctaaaagt tatcaccgtc aatattcagc cggtacatat
    22801 ggcgattatg gaaggggaga cggagatcta cctgaccgaa caacaggcgc tggtagagcg
    22861 ttttaacgat gtgccgctgt ggatccgtcc gcaaagtttc ttccagacta atccggcggt
    22921 cgccagccag ctgtacgcta ccgcgcgcga ctgggtacga cagctgccgg ttaaacatat
    22981 gtgggatctg ttctgcggtg tggggggctt tggtttacac tgcgcgacgc ctgacatgca
    23041 gttaaccggg atcgaaattg cgctagaggc cattgcctgt gcaaagcagt cagccgctga
    23101 actgggctta acgcgtctgc aatttcaggc gctggactcc actcagtttg ccaccgctca
    23161 gggggaggtg ccggagctgg tgctggttaa cccgccgcgc cgcggcattg gtaaaccgct
    23221 gtgtgattat ctctcaacga tggcaccgcg ttttattatc tactccagct gtaacgccca
    23281 aaccatggcg aaagatatcc gcgaactgcc aggttaccgc atcgaacggg tacagctttt
    23341 tgatatgttc ccgcacaccg cgcactatga agtgctgacg ctgctggtaa aaatgtaaat
    23401 acatcattca aatcaatatc acctcggcat acatccatcc aaaataataa tcgtgatcga
    23461 tcgattaata ataaccgatc gatcacggtt atatattgct ctatttttgc aaatcaaatc
    23521 acaaataatc aaataagatc tttaattgaa ctgttttgat tactacagtg cacttaccga
    23581 atacatacag aggcattgct tagcgaaaca caaaatttcc tgagacatta ttaacgtcag
    23641 cagaaggttc atgcattgac aacggaaatg tacgcgtatg gagggcgttc cgacgatgtt
    23701 tgccaaattc atagatgtta ttcaaacgtt tttaactgaa ccggcaattt taattggttt
    23761 gctggtgggt attggttatg cccttgataa gaaatcgcca ataaaaatta ttaccggcat
    23821 ggtcagcgcc atggtgggat taatgatggt gttatttggt ggtttccagt tttccgcaac
    23881 atttaaaccg gtagcagaag ccgtaagtaa agcctatggt gttcacggat atttaatgga
    23941 ttcctacgca atgaaagcgg caacgcaaat tgcattgggg gataattttg gttatgtcgg
    24001 gtatgtattt gtactggcgt tttttaccaa cctgctgctg gtattatttg gtcggtatac
    24061 tggtgccaaa gggatatttt tgacagggaa taccggtgtt tcacattctc aggcggtatt
    24121 atggttgatt gtcttttggc tgggttttag ctggactaca tccattatta ttgccggaat
    24181 attaacgggt gtattctggg cgttctccac cacgctcatt gttaagccca ttgccaaagt
    24241 taccaaagat ggcggtttta ccattgctca taaccagatg ttgggattgt ggtttttctc
    24301 caaattcgcc cataaatttg gtgaccctga aaagcacgat gctgaaaacc tgaaacttcc
    24361 cgggtggctg gcgattttta accataacgt aacggctatc gccattgtga tgacgctgtt
    24421 tgtcggtggt ttcttgctat caacgggtat cgataatgtc cagttaatgg cgaaaggcaa
    24481 gccctggtac atctatatca tcaaccttgg cttacagttc tccatgtata tggtcattct
    24541 gctgcagggt gtgcgcatga tggtgggcga aattaacggt tcgtttaaag gctggcaaga
    24601 tcgctttatt cctaacgcta ttcctgctgt cgatgttgca gcgctgttac ctttttcacc
    24661 caatgccgca acgttaggct tcgtcttctg tacttttggc accatttttt cgatggggat
    24721 cttattactg gttcacagcc caattatggt attgcctggc tttgtacctc tgtttttctc
    24781 tggcggtcca attggcgtat tagcaaaccg catgggcgga taccgttccg taattatctg
    24841 tacgttcttg ctgggtatta ttcagacctt cggtacggtg tgggctattc cgttaaccgg
    24901 gcttgctgaa aatggcgtcg gctggacagg aatatttgac tgggcaaccg tatggcctgc
    24961 tatttgtgaa gttctgaaat ttatcgctgc aacattccat cttggtcctt acgcgggtta
    25021 attaaatatt aagggtttaa aaatgaaaga tacaattaat attctgtttg tctgtggtta
    25081 cggtgtaggc agtagcgtta tgttacaaac ggtcgttaaa aaagcgctga ccaaatatga
    25141 tttcaaagtt gaaatggaac ataccgccgc aggtgaagtc ggcggattta ccgactgggc
    25201 ggatattttt gctatttcca aaaaattact cgatgtggtt agtcttgacc cgaaacaagg
    25261 gcaatatttg attccgatag agaatattat ggacggtgaa actattggtc agcaaattta
    25321 tgaagtggtt cagcaacatt ttccgcattt aattaatcaa taaggtaggg cgtaatgaga
    25381 gcgattattc tgctgttcga tagtctgaat aaacgttatc tgccacctta tggcgatgcg
    25441 ttaaccaaag cgcctaattt ccaacgtctg gcggctcatg ccgccacctt tgaaaacagt
    25501 tacgtcggca gtatgccgtg tatgcctgcc cggcgggagt tgcataccgg gcgatgtaac
    25561 ttcctgcatc gggagtgggg gccgttagaa ccgtttgacg attccatgcc ggagctgctt
    25621 aaaaaggcgg ggatctatac ccacctgata agcgatcatt tgcattactg ggaagatggc
    25681 ggcgggaatt atcataatcg ctacagttcg tgggagattg tgcgcggaca ggaaggcgat
    25741 cactggcatg caagcgttgc gcaaccgcct attcccgagg tgctacgggt gccgcaaaag
    25801 cagaccggtg gcggtgtttc tggtctgtgg cgccatgact gggcaaaccg ggaatatatt
    25861 cagcaggaag cggattttcc acaaactaaa gtgtttgatg caggctgtgc ctttatccac
    25921 aaaaatcacg cggaagataa ctggttattg cagatcgaaa cgtttgatcc gcacgagccg
    25981 tttcacacaa cggaagagta tctttccttg tatgaagata actgggatgg accgcattat
    26041 gactggccgc gtggccgggt gcaggaaagc gacgaggccg tggagcacat ccgttgccgg
    26101 tatcgttcgc tggtgtcgat gtgcgatcgc aacctggggc gcattctcga cctgatggat
    26161 gagcacgatc tgtggcgaga taccatgctg attgtcggca ctgaccacgg ctttttactc
    26221 ggtgagcatg gctggtgggc gaaaaaccag atgccttatt acaacgaagt tgccaataat
    26281 ccgttgttta tctggaaccc gcgcagtggt gtaaaaggag agcggcgaca ggcactggta
    26341 caaatgatcg actgggcgcc aacgttgtat gacttttttc aacagccagt gccgcccgat
    26401 gtgcaggggc aaccgctggc gaaaacggtc agtcacgatg aaccagtacg cagctcggcg
    26461 atgtttggtg ttttcagtgg tcatgctaac gtaactgatg ggcgttatgt gtatatgcgt
    26521 gcagcgctgc cggggcgtga ggatgatatt gccaactaca cgttgatgtc ctgcaaaatg
    26581 aacagccgct atccggtgga tgagatgcgg gctttatcgc tggccccacc gtttcgtttt
    26641 accaaagggt tacaggtatt acgcatcccg gcacaggaaa aatataaggg gttgaatcag
    26701 tttggtcatt tgctgtttga tctgcaaaac gatccgcagc agctacatcc gattcatgat
    26761 gatgtgatcg agtcccggat gatcgcgttg ctgattcagt tgatgaaaga taatgatgcg
    26821 ccgccagagc agtttcagcg cctgggatta gcgtagagaa gtgattttgc cggagtgggt
    26881 gttcgcccac tccggcaaat gaataattat tgcgggaacc actgatcgct gatttgttga
    26941 tatgtaccgt cagctttaat tgctgccagc gcgttattca gtttttccag cagagctttg
    27001 ttatccggac gtacagcgat acccagaccg gtgccaaagt attgcggatc ggtcactttt
    27061 tcagtagcaa cgcccagttg cggattggtt ttcagccatt cattaaccac cgcagtgtca
    27121 ccaaatacgc catcaatacg gctatttttc agatcgataa aggcattttg ataactgtca
    27181 taggagaccg ttttcacttc cgggtgctga tcctgaatat atttctggtg cgtagtaccg
    27241 ttttccatcc caatgcgttt gcctttcagg tcggcaaacg ttttgtaggt atcttttttg
    27301 gcaatcacga cggctgagtt ttcgtaatag ggcgtggtga acgatacctg cttgctacga
    27361 tctggtgtga tatccatacc ggagattacg gcgtcatatt tcctgaattt cagtgacggg
    27421 atcaggctgt cgaacgcgtg attggtaaaa gtacattctg cctgcatctg tttgcataac
    27481 gcttttgcta ggtcgatatc aaagccgacg atctggttat tggcatccat cgactcgaac
    27541 ggcggatagg tcgctgatac gccaaaattg attttctctg cggcagaagc gccgaacgca
    27601 caggatgcca gtaatgcggc aagaactaac tttttcatga tggaactccc gtctgtcaat
    27661 cttatgatta ttggccgtgt ctgcggcatg ggataacaat gccatcaagt
//
LOCUS       DAZKFZ010000046        27524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000046 DAZKFZ010000000
VERSION     DAZKFZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(183..1775)
                     /gene="lsrK"
                     /locus_tag="ACHOQW_001791"
     CDS             complement(183..1775)
                     /gene="lsrK"
                     /locus_tag="ACHOQW_001791"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP9572877.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1854..2807)
                     /gene="lsrR"
                     /locus_tag="ACHOQW_001792"
     CDS             complement(1854..2807)
                     /gene="lsrR"
                     /locus_tag="ACHOQW_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP9572878.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3056..4591
                     /gene="lsrA"
                     /locus_tag="ACHOQW_001793"
     CDS             3056..4591
                     /gene="lsrA"
                     /locus_tag="ACHOQW_001793"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP9572879.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4585..5625
                     /gene="lsrC"
                     /locus_tag="ACHOQW_001794"
     CDS             4585..5625
                     /gene="lsrC"
                     /locus_tag="ACHOQW_001794"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP9572880.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAVLGAWFL
                     TQIDSVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSV
                     KPASSGKKREAA"
     gene            5625..6617
                     /gene="lsrD"
                     /locus_tag="ACHOQW_001795"
     CDS             5625..6617
                     /gene="lsrD"
                     /locus_tag="ACHOQW_001795"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP9572881.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGYPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6629..7651
                     /gene="lsrB"
                     /locus_tag="ACHOQW_001796"
     CDS             6629..7651
                     /gene="lsrB"
                     /locus_tag="ACHOQW_001796"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP9572882.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7678..8553
                     /gene="lsrF"
                     /locus_tag="ACHOQW_001797"
     CDS             7678..8553
                     /gene="lsrF"
                     /locus_tag="ACHOQW_001797"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP9572883.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8577..8867
                     /gene="lsrG"
                     /locus_tag="ACHOQW_001798"
     CDS             8577..8867
                     /gene="lsrG"
                     /locus_tag="ACHOQW_001798"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP9572884.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8924..9682
                     /gene="tam"
                     /locus_tag="ACHOQW_001799"
     CDS             8924..9682
                     /gene="tam"
                     /locus_tag="ACHOQW_001799"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP9572885.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9686..10600)
                     /gene="yneE"
                     /locus_tag="ACHOQW_001800"
     CDS             complement(9686..10600)
                     /gene="yneE"
                     /locus_tag="ACHOQW_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP9572886.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10797..12248)
                     /gene="uxaB"
                     /locus_tag="ACHOQW_001801"
     CDS             complement(10797..12248)
                     /gene="uxaB"
                     /locus_tag="ACHOQW_001801"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9572887.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12475..13893)
                     /locus_tag="ACHOQW_001802"
     CDS             complement(12475..13893)
                     /locus_tag="ACHOQW_001802"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9572888.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14032..14391)
                     /gene="yneG"
                     /locus_tag="ACHOQW_001803"
     CDS             complement(14032..14391)
                     /gene="yneG"
                     /locus_tag="ACHOQW_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP9572889.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14391..15317)
                     /gene="glsB"
                     /locus_tag="ACHOQW_001804"
     CDS             complement(14391..15317)
                     /gene="glsB"
                     /locus_tag="ACHOQW_001804"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP9572890.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15381..16769)
                     /gene="sad"
                     /locus_tag="ACHOQW_001805"
     CDS             complement(15381..16769)
                     /gene="sad"
                     /locus_tag="ACHOQW_001805"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP9572891.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16870..17751
                     /gene="yneJ"
                     /locus_tag="ACHOQW_001806"
     CDS             16870..17751
                     /gene="yneJ"
                     /locus_tag="ACHOQW_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9572892.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17829..18944
                     /gene="yneK"
                     /locus_tag="ACHOQW_001807"
                     /pseudo
     CDS             17829..18944
                     /gene="yneK"
                     /locus_tag="ACHOQW_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19095..20285
                     /gene="ydeA"
                     /locus_tag="ACHOQW_001808"
     CDS             19095..20285
                     /gene="ydeA"
                     /locus_tag="ACHOQW_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP9572893.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQMQ"
     gene            complement(20310..20975)
                     /gene="marC"
                     /locus_tag="ACHOQW_001809"
     CDS             complement(20310..20975)
                     /gene="marC"
                     /locus_tag="ACHOQW_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP9572894.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            21187..21621
                     /gene="marR"
                     /locus_tag="ACHOQW_001810"
     CDS             21187..21621
                     /gene="marR"
                     /locus_tag="ACHOQW_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP9572895.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21641..22024
                     /gene="marA"
                     /locus_tag="ACHOQW_001811"
     CDS             21641..22024
                     /gene="marA"
                     /locus_tag="ACHOQW_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP9572896.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22056..22274
                     /gene="marB"
                     /locus_tag="ACHOQW_001812"
     CDS             22056..22274
                     /gene="marB"
                     /locus_tag="ACHOQW_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP9572897.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(22305..23204)
                     /gene="eamA"
                     /locus_tag="ACHOQW_001813"
     CDS             complement(22305..23204)
                     /gene="eamA"
                     /locus_tag="ACHOQW_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP9572898.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            23399..24586
                     /gene="ydeE"
                     /locus_tag="ACHOQW_001814"
     CDS             23399..24586
                     /gene="ydeE"
                     /locus_tag="ACHOQW_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP9572899.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(24627..25022)
                     /locus_tag="ACHOQW_001815"
     CDS             complement(24627..25022)
                     /locus_tag="ACHOQW_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9572900.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            25117..26088
                     /gene="ftrA"
                     /locus_tag="ACHOQW_001816"
     CDS             25117..26088
                     /gene="ftrA"
                     /locus_tag="ACHOQW_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFP9572901.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            26196..26291
                     /gene="mgtS"
                     /locus_tag="ACHOQW_001817"
     CDS             26196..26291
                     /gene="mgtS"
                     /locus_tag="ACHOQW_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP9572902.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            26291..26395
                     /gene="mgtT"
                     /locus_tag="ACHOQW_001818"
     CDS             26291..26395
                     /gene="mgtT"
                     /locus_tag="ACHOQW_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP9572903.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(26510..27400)
                     /gene="dgcZ"
                     /locus_tag="ACHOQW_001819"
     CDS             complement(26510..27400)
                     /gene="dgcZ"
                     /locus_tag="ACHOQW_001819"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP9572904.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         6995 a         6945 c         6627 g         6957 t
ORIGIN      
        1 aaataagggt tatgcttaat gctttaagaa aaatagcaat ttccccttga atatagtact
       61 ggtgatgaaa cgatgaatct gcaagctggc atttaacaag ccagctctaa aagaagggaa
      121 ataagaataa ctatactcaa aaactaacag ccacggtcat catgatgtgg ctgtcaatga
      181 aactataacc caggcgcttt ccataacgac gtcgtcagtc catgatcaac cagccccagc
      241 tgatcctgat aaactgcctg ccacttatcg cgtgaatcct gataaagttc atgcttttcc
      301 gggtctggtg tgtgcgtccg ttcccagcga accaggcgtt ctccggtttc tgccattgat
      361 gaaaaaattc cggcaccgac gccagctgca atggcacatc ctaatgcagt ggcttctttg
      421 accaccggaa tattgacggg taatcccgag acatcagcga gaatttgact ccataatttc
      481 ccttttgaac ctccgcctgc aaagactaac gatgaaggat gaatagtcga aaaatcagca
      541 atttgctgca agttacacgc tgatacaatc gccgcatttt cttccagcgc acggaacaat
      601 gtcgctttgt tacatttatc cggatcaatg gacaaattaa taaaggaagg cgcagcgtga
      661 taccaggttt taaagcgcat tctgtcggag aagatcggca ttacgcccca cgacccaggc
      721 ggcacccgac tggccatctc ttccagcagc gtataggtgt cgatgcctaa acgttcggca
      781 atcagttttt cttcggcaca gaaagcatcg cggaaccagc gcatggtgag tccggtaaaa
      841 aagcttatag attcagcttg taccatgcca ggaataacat gaggattaac gcgcacgttc
      901 atttctgggt ctgtcaccgg cgcggctaaa tttacaactt gctgccagaa tgtgccgcca
      961 agaaccgcgg tttgtgccgg acgcacaacg cctaacccca ggcaaccaag ctgcacgtcg
     1021 cctcctccaa cgaccaccgg agtgcccacc ttcagaccgc agagttccgc cgcttgtgaa
     1081 cttaccacgc ccagcaatgt gccggtttct ttgacaggag aaagaatatc ggcacgcagg
     1141 ccagccatat ccagcaatgc aggtttccag tcacggatgg ttagatcaag aagtcccgtg
     1201 gtgccagcgt tagagggatc caccgcaagt tcgccgctga gcatataggc cagccagtcg
     1261 ctgatcatgg tgatggttga tgcctgacgg taaatatcgg aacgatggtg cgccagccaa
     1321 agtaatctgg ggatggcact taaagccagt gtttgtccgg tcgcgcgata aacttcgttt
     1381 tcaaaggtat tgttgtgcag ttctttaagt tcgctaactt cgcgcgccgc tctggcatcc
     1441 acattggcgc aggcccagat cggtgttcct tcattgttat ataaaacaat gccttcacgc
     1501 atcgaacatg ccgaaacggc agcgatatac tccggggcta tgccggcgtt gtgcagcgcc
     1561 tggcgcatac actcacacgc cagttgccag tttttgttga gatcaaattc catagaacca
     1621 ggaacgtccg gtactgccag atgccgccac tccgcctgtc ccactgctat ttgattgcct
     1681 tccaggtcga atatcacagc ccgaatactt ccggtgcctg catccagcgc catcaggtag
     1741 tactttgatt ctgaaggggt aaagagtcga gccattagat tatcctcggc ttatgcgttc
     1801 tgtctgttcc tctatacgtt ctccatcatt cccggtaata aggtcatgca aatttaacta
     1861 cgtaaaatcg ccgctgctgt gtcctgatcg gtaaccagtg cgttgatata accgcctttc
     1921 attgcagcgg caattgcttc ggctttattt tctccccctg ccacgccaac ccggacgggt
     1981 atggtcttca gcgcgcttaa aggtaagcca atcagctcgt tatgtatttt aatgtccgtg
     2041 acaacgtcac cttttgcatc aaaaaagtag cctaaaatgt cgccaaccgc cccttttcgg
     2101 ccaatcatta actgttcgcc ctggctgata taaccggagc gaatgatcgt cgcgtcgtct
     2161 tgttgactaa cggccccaat gccgacaatc gccacatccg ctgcttgcgc ggctaacaga
     2221 acatctttga cacaattttc attttttagc gtacgggcaa tgtcagcgga ggatgcccgc
     2281 aacggagccg gaataatatt cacactgcac gccgcattaa gctgtccgat acccgtcata
     2341 taagaaccga cgccaccgga gagcgtgacc aggcgaattt gctgtgacga aataaaacca
     2401 cttaagcgtt gcagcgtatt catggttgcc tcgccaaaac caatcgccag catctgttgt
     2461 ggttgaagta aactcatcaa catatgcgcc gcgcctatcc ccagtcgccc gccgacatca
     2521 gcatccgcaa gcccagggat cacccggaca tgttgcagcg aaaactgacg acgtaattgt
     2581 gtttcatatt ccagacagcc ttcaaagcga gaattaatct gcacgcgaat gatgccggac
     2641 tgatgccctt tctccagtaa tcgcgacact ttcaaacggg tcaggccgag acgatcgctg
     2701 atctcactct gggtcagccc gtcgtggtag taaaaccacg cgatccgcgc gacctgttct
     2761 tcttcacaca ttccctgttc tgaaattacc gaatcgttga ttgtcataat tcattcttca
     2821 ctttgaacat atttaaatct ttaatgcaat tgttcagttc ttgcgcattt atatctgtga
     2881 tggcaaccac agtttgactc tacgagcatg aacaaacgca accgtgaaaa tcaaaatagt
     2941 ataaattgtg atctattcgt cggaaatatg tgcaatgtcc acctaaggtt atgaacaaat
     3001 taaaagcaga aatacatttg ttcaaaactc acctgcaaaa ctgaacgggg gaaatatgca
     3061 aacgagtgat acccgcgcgt taccgctact ttgcgcccgc tcggtttata aacagtattc
     3121 aggggtcaat gtcctgaaag gcatcgattt tacgttgcat cagggggagg tccacgccct
     3181 gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg gtattacctc
     3241 tgctgatagc ggtacgctgg agattggggg caacaactac gccagattaa cgccagttca
     3301 tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc caagcctgtc
     3361 gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc agaaaatgaa
     3421 gaacttgctg gcggcgctgg gctgccagtt tgatctgcat agtctggcag gatcgctgga
     3481 tgtcgccgat cgccaaatgg tggaaatcct ccgcgggctg atgcgcgact cgcggattct
     3541 gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct tgtttactcg
     3601 cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata agctgccgga
     3661 aattcgccag attgccgatc ggattagcgt gatgcgcgac ggaaccatcg ccttaagcgg
     3721 caaaaccagc gaactgtcta ccgacgacat tattcaggcc atcaccccag cggtacggga
     3781 aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc gcccacaaca
     3841 tgccgcagga acgtcggtgc tgacactgga aaatctgact ggcgaaggtt tcaggaatgt
     3901 cagcctgacg ctaaatgccg gagaaattct gggtctggct gggctggtgg gtgccggacg
     3961 cacagaactg gccgagacgc tctatggtct acgtactttg cgtggcggac gcattatgct
     4021 gaatgataaa gagatcaata gattatccac tggagaacgt ttactgcgcg gtctggttta
     4081 tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg cctggaacgt
     4141 ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag ataatgccac
     4201 cctggaactt tatcgtcggg cgctgaatat taaatataac caaccggaac aagctgcacg
     4261 gacattatcc ggtggtaacc agcaaaaaat cctgattgcc aaatgcctgg aagcctcgcc
     4321 gcaagtattg attgtcgatg agccgacgcg cggcgtggat gtctcggcgc gtaatgatat
     4381 ctaccaactg ttgcgcagca tcgccgcaca gaatgtggct gtgctgctta tctcctctga
     4441 tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg gcgaaattac
     4501 ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg ttgccttcgg
     4561 cgatagtcag cgtcaggagg cggcatgctg aagtttattc agaacaaccg tgaaatcacg
     4621 gcactgctgg cggtgctgct gctgtttgta ttacccggtt ttctcgaccg ccagtattta
     4681 agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct gctggcaatg
     4741 ggcgcgacgc tggtaatgct tacgcgcaat attgatgttt cagtgggttc gattaccgga
     4801 atgtgcgcgg tgctgttggg gatgttactg aacgcaggat attcactacc tgttgcctgt
     4861 gtcgcgactt tactgcttgg tttgctcgcg ggatttttca acggtgtcct ggtcgcgtgg
     4921 ctaaagatcc ctgccattgt tgccaccctt ggcacgttag ggttgtacag aggcatcatg
     4981 ttgctgtgga ctggcggtaa atggattgaa gggttacccg ccgaactgaa acagctctcc
     5041 gccccgctgc tgcttggcgt ttcagcaatt ggttggttaa cgataattct ggtggcattt
     5101 atggcctggc tgctggcgaa gacagcgttt ggacgcagtt tttatgccac gggcgataat
     5161 ttacagggcg ctcgtcaact gggcgttcgt acggaagcca ttcgcattgt ggcattttcg
     5221 ttgaacggct gcatggcggc actggcggga attgtgtttg cttcgcagat tggttttatc
     5281 cccaaccaga ccggtaccgg gctggagatg aaagcaattg cagcctgcgt gctgggcggc
     5341 attagtttgc tcggcggttc cggtgcgatc atcggtgcgg tactcggtgc ggtactcggc
     5401 gcatggttcc tgacgcagat cgatagcgta ctggtgctgt tgcgcattcc ggcatggtgg
     5461 aatgatttta tcgcgggtct ggttctgctg gcggtgctgg tgtttgatgg acgcctgcgt
     5521 tgtgcgctgg aacttaatct gcggcggcaa aaatatgccc gctttatgac gccaccgcca
     5581 tccgttaaac ccgcttcgtc aggtaaaaaa cgggaggctg cataatgcgt attcgctacg
     5641 gttgggaact ggctcttgcc gcactgcttg ttattgagat tgtctcattt ggtgcaatta
     5701 acccgcggat gttagatctc aatatgttgc tgttcagcac cagtgacttt atctgcattg
     5761 gcattgtcgc cctaccgctg acgatggtga ttgtcagtgg cgggatcgat atttcgtttg
     5821 gttcgaccat cggcctctgc gccattgcat tgggcgtact gtttcaaagt ggtgtgccga
     5881 tgccgctggc gattctcctg accttactgc tcggcgcatt gtgcgggctg atcaacgccg
     5941 gattaattat ctataccaaa gttaacccgc tggtgattac gcttggcacg ctgtatctgt
     6001 ttgccggaag cgccctgctg ctttccggga tggccggggc gacggggtac gaaggtattg
     6061 gtggataccc gatggcgttt acagatttcg ctaacctgga tgtgttgggg ctccccgttc
     6121 cgctgattat cttcctgata tgtctcctcg ttttctggct ctggctgcat aaaacccatg
     6181 ccggacgtaa cgtgtttttg attgggcaaa gcccgcgcgt ggcgctttat agcgcgattc
     6241 cagttaaccg taccttatgt gcgctctatg ccatgacggg gctggcgtct gcggtcgccg
     6301 ctgtgctgct ggtgtcgtat tttggttcag cacgttccga tctcggtgcg tcgtttctga
     6361 tgcccgccat caccgccgtg gtgcttggcg gtgccaatat ttatggtggt tccggtgcca
     6421 ttatcggcac cgccattgcg gttttattag tgggatattt gcaacaaggt ttgcaaatgg
     6481 caggagtgcc aaatcaggtg tccagcgccc tttccggtgc gctacttatc gtcgttgtcg
     6541 taggtcgttc cgttagcctg catcgccagc aaattaaaga gtggctggcg cgtcgggcca
     6601 ataacccatt gccataaagg atatcttcat gacacttcat cgctttaaga aaatcgcctt
     6661 acttagcgct cttggcattg ccgcaatctc tatgaatgtg caggccgcag agcgtattgc
     6721 atttattccc aaactggttg gagtgggatt ttttaccagc ggtggcaacg gcgcacaaca
     6781 agcgggtaaa gagctgggcg ttgatgtgac ctacgacggg ccgacagaac ccagtgtttc
     6841 tggtcaggta cagttgatta ataacttcgt caatcaaggt tataacgcca ttatcgtttc
     6901 tgcggtttcg cctgatggct tgtgtccagc actgaaacgc gccatgcaac gtggtgtgag
     6961 agtgctgacc tgggactctg atactaaacc ggagtgccgc tcttactaca ttaatcaggg
     7021 aactcccgcc cagttgggag gtatgttggt ggatatggcg gcgcgtcagg tgaataaaga
     7081 caaagccaaa gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa accagtgggt
     7141 gaaagaagcg aaagcgaaaa tcgccaaaga gcatcctggc tgggaaattg tcactacgca
     7201 gtttggctat aacgatgcca ctaaatcatt acaaaccgca gaaggaatat taaaagcgta
     7261 tagcgatctc gacgccatta tcgcccccga tgccaatgcc ctgcccgctg ccgcacaagc
     7321 cgcagaaaac ctgaaaaatg acaaagttgc gattgtcgga ttcagtacgc caaacgtgat
     7381 gcgtccatat gtggaacgcg gcacggtgaa agaatttggc ctgtgggatg tggttcagca
     7441 aggcaaaatt tcagtttatg tcgcggatgc attattgaaa aaaggatcaa tgaaaacggg
     7501 cgacaagctg gatattcagg gcgtaggtca ggtggaagtc tcgccaaaca gcgtccaggg
     7561 ctatgactac gaagcggatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa
     7621 caaagagaat atcggcaaat acgatttctg atgtgcatta cttaaccgga gtaagttatg
     7681 gcagatttag acgatattaa agatggtaaa gattttcgta ccgatcaacc gcaaaaaaat
     7741 atccctttta ccctgaaagg ttgcggtgcg ctggattggg gaatgcagtc acgcttatcg
     7801 cggatattta atccgaaaac gggtaaaacc gtgatgctgg cttttgacca tggttatttt
     7861 cagggaccaa ctaccggact tgaacgcatt gatataaata tcgccccgct gtttgaacat
     7921 gccgatgtat taatgtgtac gcgcgggatt ttgcgcagcg tagttccccc ggcgaccaat
     7981 aagccggtgg tactgcgggc gtcaggtgcg aactctatcc tggcggaatt aagcaatgaa
     8041 gccgtggcgt tatcgatgga tgacgccgtg cgcctgaaca gttgcgcggt ggcggcgcag
     8101 gtttatatcg gcagcgaata tgaacatcag tcgatcaaaa atattattca gctggtcgat
     8161 gccggaatga aagtgggaat gccgaccatg gccgtgactg gcgtgggcaa agacatggtg
     8221 cgcgatcagc gttatttctc gctcgcgact cgaatcgccg ctgaaatggg gacgcaaatt
     8281 atcaaaacct attatgtcga aaaaggtttt gaacggattg ttgccggatg tccggtaccc
     8341 attgttattg ctggcggtaa aaaattaccg gagcgcgagg cgctggaaat gtgctggcag
     8401 gctatcgatc agggcgcttc tggtgtggat atggggcgta atattttcca gtctgaccat
     8461 ccggtggcga tgatgaaagc cgtacaggcg gtggttcacc ataacgaaac ggctgatcgg
     8521 gcatatgaac tctatctgag tgaaaaacag taactgcgga tttaaggaga agaattatgc
     8581 acgtcacact ggttgaaatt aacgttcatg aagacaaggt tgacgagttt atcgaagttt
     8641 ttcgccagaa ccacctgggc tctgtacagg aagaaggcaa tttgcgcttc gatgtcttac
     8701 aggacccgga agtgaattcg cgcttttata tctacgaagc ctataaagat gaagacgcag
     8761 tggcgttcca taaaaccacg ccccactaca aaacctgtgt cgcgaaactg gaatctttaa
     8821 tgaccgggcc gcgtaaaaaa cgtctgttca atgggttgat gccgtgaggc gaatttatca
     8881 gttttatcta caattggggt aacgcgctga cgggagtaaa taaatgtctg actggaaccc
     8941 ctctttatat ctgcactttg ccgctgaacg atcgcgtccg gcggtggagc tgcttgccag
     9001 agtgccgctg gagaatgtcg attatgttgc cgatcttggc tgtggcccag gtaacagcac
     9061 cgccctgctg catcaacgtt ggcctgctgc caggataacg ggcatcgact cgtctccggc
     9121 gatgattgct gaagcgcgca gtgctttgcc agactgccag ttcgtggaag cagacatccg
     9181 caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac tgcaatggct
     9241 gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc agggcgtgct
     9301 ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc gcgaagttgc
     9361 ctgggaacaa aactacccgg atcgcgggcg tgagtcgttg gctggtgttc atgcttacta
     9421 cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccacct actatcacca
     9481 gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac gtccgtggtt
     9541 acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc agatgctgaa
     9601 agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc gtctgtttat
     9661 tgttgcccgc cgtacggagt aaatatcacg tcagttggta atggcgatcg ggaagaactt
     9721 ttgctggaat ttcggcttca tcgttcatct gtaagaggtc aatttcaata gcgttacaga
     9781 tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt tcttccgcca
     9841 gacaatccag cgaaataaaa gtataggaaa tcagcacaga gataaaaggc gtcatgtaat
     9901 gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg gtacgatgca
     9961 aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcgcag cctgccagaa
    10021 ccgctgaaat atcgttaagc cgatcgttga ggctaataaa caggatatct gaaagttgtc
    10081 cattgcgtcg ctgaaccgcc aaccactccc ccattattaa caagatgcgg ttagctggag
    10141 agttcgaagc cagtacacgc tgaagatctt cagtcttgag ataatgagcc agcacttcca
    10201 cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt tgcagccggg
    10261 caaactccct tacacttgcc gaatccggca atgtcgtttt tacctcgcgc agtaacgacc
    10321 gtgaggcaat catcaactgc ccccaaagtt tgcgcgcttc aacgtaacgg gcgtaaccgg
    10381 cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg aacggtgcga
    10441 gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaaaataaca gcgatagaaa
    10501 agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagcca tgccagacaa
    10561 aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc aggcgtggaa
    10621 aagtcgccct attaaacgtg attacgatca cattctcaag acactactta caaattacct
    10681 acaaaattac aacattaaca ggtgctgcat gtggcacctg ttaatgataa aggcttatag
    10741 gatgttgtaa ctaatataat gaaataagaa acccggtcga aaccgggttc agagtattag
    10801 cacaacggac gtacagcttc gcgcatccct ttttcgagaa tcgcatccag gtcattagcc
    10861 acctgctcga ccagaccagg cacttgcgtc aggtcctgct cccagtggtc tttttctgcc
    10921 aacacaatcg ctaccagttc ctgggtaccg ataacacggt cacgatgctg actccagagt
    10981 tgctgataac gttccagcca gtgtgcatcg tcctgtaccg gatatgtttc tccattacgc
    11041 tcaccgcgat agaacgcaat caatgccgct aatgcgaaag taaggcgcgc cggaagtgtg
    11101 ccgtttgcct tctgccctgc cagcagctgc ggcaggatgc gggtacggaa cttggtcata
    11161 ccgttgagcg cgatagacag cagctgatgc ttaatgtacg ggttacggaa acgcccggtg
    11221 actgcactgg cgaaagattc cagttcatcg cgtggcaaat ccagtaccgg aataatttct
    11281 tcgtagatgg ctttttcaac gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg
    11341 gtatccagcc ctgcctggaa cgccaccggc accaacgcgg tgtgtgcacc gttgagtatt
    11401 gccactttgc gttctttata cggtttaata tcgtcaacaa tcagcacgtt gagcggatat
    11461 ttgtccagac gcagctcggt cgctaaggat ttcggtccct gaatcacaaa caagtaaaag
    11521 tgttcagcgg tgtcgaggaa accatcgtga taacccagtt cttcttccag tttagccact
    11581 tcatcacgcg gataaccggt aacgatacgg tccaccagcg tagaacagaa gctgttagcc
    11641 tgatccagcc attgaataaa tgcttctggc agtgcccact cttgcgcata gcgcagcacc
    11701 agttcacgca acgcgtcgcc attgtagtcg atcaactcac acgggatgat gatccaacct
    11761 ttatccagcg caccgttgaa atggctaaaa cgttcgaaca gcagacgggt cagttttgcc
    11821 ggataactta ccgctggcgc gtcatcgaat ttatcgcccg catggtagct gatacccgct
    11881 tcggtggtgt tagagaaaac aaagcgcatt tccgggttgt gcgccagttt caggaattca
    11941 tcgtattcac tgtagacgct gatttcacga ttaacagagc gaatcagacg cgcgtcgctg
    12001 accgcttccc ccttctcatt caggccacgg ataatggtgg tgtacagacc atcctgcgtg
    12061 ctcagtgacg gcgggaatga agtttcaatc ggacgaacaa cgaccacacc agaattcaga
    12121 tcggtgtgct cattcaggag atctatttgc cagtcaacaa aggcgcgcag gaagttacct
    12181 tcaccaaact gaatgatacg ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt
    12241 gttttcacaa tgggtcccct tctgattagt catacaacct gtttgaattg gtacgacagg
    12301 ttagcaaact ttaatacgac gaacccctgt tttgatcaac tcctgatgat taatgagcag
    12361 ttttatgaga aaagtgtggc gcggatcatg gtttaatcga ggaaatacgc cttttcctgg
    12421 atcataaagt ggtagaacac attgcattca aatcgcgcgg aatgaataaa gatgtcagac
    12481 aacttcctca ccgtaacgca tagtgctggt acggttgcgc ccatctttct tcgagcgata
    12541 cagacaggta tcggcttcga ccatcaattt attaaagtca tcggtcagcg ttcggtacga
    12601 tgcgcgacca ctaccgacgc caatacttac cgtgagataa agcgtttttt gttgccaggt
    12661 gaatggttgc agttcaacgc ctttacggat tttttccgcc attagcagac catcgacagg
    12721 attcactgac ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca ccagcccctt
    12781 atcaccgaca atcttctgaa tatgccgggc aaacacgctt aacactttat cgccacattc
    12841 atgtccatag ttatcgttaa tacttttgaa atagtcgata tcaagtagca tgacagtcag
    12901 atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg accgtgagta
    12961 aacctgagtc agaaaatcaa agtcggctcg cagcgcaact tgcttcatta gcgaattgat
    13021 cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa taccgagacg
    13081 tgcggagaac atttgcggaa tggagaacgg cgaaccgacc gagatatcaa tcaccgaatt
    13141 agccaccagt acgatttcca ccgcaccggt gacaaaggtc aacagacatg ttacctgcgg
    13201 cgtatagcgc actgcacacc agattaatgc aggcagcggg aacgccagac tccccgctcc
    13261 gccaatgact accgaggcaa taaccgacac aataagcgcg atggcgggca tcatctgttc
    13321 tgctttaaag cgcggcagca ctccaggaat agccaacgtc agcatacaag gcacgatcaa
    13381 cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga aatccagact
    13441 atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg caatcagaca
    13501 gtaattaaat agccgtaagg cacttactgg ctcatattta tttttcccca aacgcttatc
    13561 ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat tgatagccag
    13621 tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca cataactgat
    13681 cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg ccattacgcc
    13741 attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac caataaaata
    13801 gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac cttcctggaa
    13861 cacacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac aattgcgggt
    13921 ggcaattatc tcaacgtaat aagtaaattc tcataaagga tagcatgttc gctgacagcg
    13981 gcggcaaata aaggtaccct gggaatgcta ttttgtcgtt ttccgcaatt ctcatggctg
    14041 attcatctgt acaaccagcc agtgatagat taccgccacg atataacgtt gttgttcttc
    14101 gcttagcgaa acaccatgcg ggatgttatg ccacttagcc agacaaccgc gacagcaggt
    14161 cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt gcttaccgtc
    14221 attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat gttgttcgat
    14281 gactggcgcg cctttctcca gacaatactg acgctctttc atgcccaaac gaaagcgaga
    14341 tcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agagactgca ttaataaacc
    14401 gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag cgagttacct
    14461 gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac aatcgccaca
    14521 ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa ctcccccgcg
    14581 ttctggtaca taccactggt cgccatcagc gcgttaattt gccgtgcctg cattggcgtc
    14641 accacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac aaacgtccgg
    14701 gccagctcta cacagctcat tttcagagcg cagtaatgaa aatagttttg cagaacggtt
    14761 gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc cgcatttcgc
    14821 gcggaatgtt caaattcgga acgcgctacc accgtatcgt aggaaatatc agacacaccg
    14881 cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttaatcg cccttgcaac
    14941 atatcgcaga ccaccagcgc accagcatta ataaacggat tacgcggaat accctgctcc
    15001 atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc gacgcgttgc
    15061 cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac tttggaaata
    15121 gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc gtcaacggta
    15181 cagatagcaa tgcccaatcg ggaaccgtct actgtagcca gcgccggaat ataatccgcg
    15241 actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa aattgcatta
    15301 tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc tgcgagtata
    15361 tcagagctga atatgtcgcg tcagatccgg tctttccaca ccgtctggat attacagaat
    15421 tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac gccaccaaag
    15481 gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca ttccagacgt
    15541 gccgccatct gtctggcctg tgtttcatcg gtggtaaaaa tggtcgctga aaggccgaac
    15601 tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaatggt gattgccgca
    15661 acggggccaa acatttcttc ccgaaaagcg gtcatttctg gggtaacatt cgccagaacc
    15721 gttggcggat agtagttacc tgccccagcc atcttttccc cgcccagtaa caaacgcgca
    15781 ccctgcgcca gggttttctc cacctgatga tgcagctcat cacgtaaatc gaaacgagcc
    15841 attggtccga gagcgttctc ttcgtcacgg ggatcgtcca ttttcaaggc tgccgcagct
    15901 gccacaaaac gttcggtaaa tgccgaagca attccctctt cgataataaa gcgttttgcc
    15961 gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt aactgccagt
    16021 tccagatcgg catcgttaag cacaataaat ggatccgaac cgcccagttc cagtacgcat
    16081 tttttcagtg ccgctccagc ctgtgcacca atagccgctc cagcacgaac acttccggtc
    16141 accgtgatag cagcaatgcg cgagtcttta atcatctgag tgacaccgtc attgtcggca
    16201 ttcagccagc catatacgcc ttgtgggatt cccgcatctt taaacacctg ggcaatgagc
    16261 tgagcacagc ccatcacatt cggcgcatgt ttaagtaagt atccgttacc agcaagaatg
    16321 atgggaacag cgccacgcat cacctgccat aacggaaaat tccacggcat aatcgccaga
    16381 atcgtcccca acggtcgata ctcaataact gcctgctgat tttccaccag cgtaggttcc
    16441 gccttcagca ttgccggacc atgttctgca taccagtcac acaaattcgc cgatttcgcc
    16501 acttcagcgc gcgcctggtt gattggtttg cccatttcgc gggtgatcat ttgcgccatt
    16561 tcttcgctac gggcgcgcag agccttaccg atatcacgca gtttttcagc acgataatct
    16621 atatttgtct cgcgccagtc acgaaagcct gctgccgcca gctgaagtgc gttttcgata
    16681 tcatcagcgc cagcccacgg caacacagaa agttgttcac ccgtggcagg atttatcgaa
    16741 attgcatgag ttgccggagt aatggtcatc ggggtatctc ctttatgagt catggtatga
    16801 agatacgcag atttactctt gctttaaaat gaataatatt aagccactta ttcacgaatc
    16861 gagaatatta tggatctgac ccaactggag atgttcaacg cggttgccga agctggcagc
    16921 attacgcagg ctgcagcaat agtgcatcgc gtgccgtcga atctcactac ccgtctacgc
    16981 cagctggaaa cagaactggg ggtagatctg tttattcgcg agaatcagcg tttacgtcta
    17041 tctcctgccg ggcataactt tttacgctat agccaacaaa ttctcgcgtt agtggatgaa
    17101 gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat tttctctggg ttcgctggaa
    17161 agcaccgcag cagttcgcat tccggccacg ctggcggaat tcaaccgtcg ttatcccaaa
    17221 attcagtttt cactttccac cggcccttcc ggcactatgc tggaaggtgt gctggaggga
    17281 aaacttaatg cggcgtttat tgatggaccc attaaccata ctgccattga cgggataccg
    17341 gtataccgcg aggagctgat gatcgtcacg ccacaaggtc atgcgccagt aatccgtgcc
    17401 agtcaggtta atggcagtaa catttatgcc ttccgcgcca attgttcgta tcgtcgccac
    17461 ttcgagagct ggtttcatgc tgacggtgcc gctccgggaa ctatccatga gatggagtct
    17521 tatcacggaa tgttggcctg tgtggtcgca ggagcaggta ttgcgcttat tccgcgctct
    17581 atgctggaaa gtatgccggg gcatcaccag gttgaagcgt ggccgttagc tgagcaatgg
    17641 tgttggctaa caacctggct ggtctggcgt cgtggtgcga aaacgcgtcc gctcgaggca
    17701 tttattcaac tactggatgt gcctgactcg acaaaacagg gttaccaatg agctattttt
    17761 gatagttctt gcggctaata tgctctatat agtgatgttc cgatgactta tgactatatg
    17821 gggcaaatat ggttacgcca gtaagcatca gcaattacat atctcttcct gatgattttc
    17881 ccgttcgtaa tattgcaccg caggtaaaag aagttttaaa ggattttatt gatgcactta
    17941 gtacaataat atgtaatgaa gagtggcgca cgtctttaaa catcaactca gccacaaaaa
    18001 agatatttaa taatcttgac aacttatcat atattcagag gacttctttt cggggtaacg
    18061 acacgctata caatgaaaag gttcagttta aactcactta tcccgccagg aatggaagac
    18121 acaaagaaaa tattgaattt caggtagtaa taaatttaag ccctatttat ttagataatt
    18181 tccgccatga tggagaaatt aatatttttt gcgctcccaa cccaaagcct gttactatgg
    18241 ggcgcgtatt tcagaccggc gtcgagcgtg tgctctttct gtttctgaat gattttattg
    18301 aacaattccc aatgatcaac cctggcgttc ccatcaaaag agcgcataca ccacatattg
    18361 aacccctgcc tccggatcac cataccgctg cagattactt acgccagttt gatttgcttg
    18421 tcctgaattt tatctctcgc ggtaattttg tcatactccc ccgattatgg aataactctg
    18481 aggttcacag atggttcgtc aataaagatc ctaacttgat caccgccatt ctcgacataa
    18541 cggacagcga attaaaagag gatttattgc aaagcctgat ggattcatta ggttctaaca
    18601 aacatgtact accctaagtc tgcatctgtt ttttatccct tttagcagaa caagaatctc
    18661 ctcattttca ggacttgttt ttatttttcg ccaatatgtt gttacactat caccaattta
    18721 tgaatcccaa tgaaagtgat ttgaatgacg tgttgatgcc agcatcatta agtgatgata
    18781 aaattatcaa acatatggcg cgtaagaccc tcaaactgtt tgtaaaaaat gaaacaccgc
    18841 caaaagttac tcatgaagat ctggtgaaaa acaggcctcg ctcccctgtc agaccgccta
    18901 tacccgcaac ctccaaaacg ccagacattc ctgatcgtca ttaaaccgtg atgtttacca
    18961 tctctctgac gcgtgaaaga atcagcgtca gagaaacgga aaacgcgatc cagatcacaa
    19021 atgcattgta ttcacatcat taaccgtttt aagatcattt catcactttt tcgcaactca
    19081 cccgataatc tgttatgaca acaaacactg tttcccgcaa agtggcgtgg ctacgggtcg
    19141 ttacgctggc agtcgccgcc ttcatcttca acaccaccga atttgtccct gttggcctgc
    19201 tctctgacat tgcgcaaagt tttcacatgc aaaccgctca ggtcggcatc atgttgacca
    19261 tttacgcatg ggtagtagcg ctaatgtcat tgccttttat gttaatgacc agccaagttg
    19321 aacggcgcaa attactgatc tgcctgtttg tggtgtttat tgccagccac gtactgtcgt
    19381 ttttatcgtg gagctttacc gttctggtga tcagtcgcat tggtgtggct tttgcacatg
    19441 cgattttctg gtcgattacg gcgtccctgg cgatccgtat ggctccggcc gggaagcgag
    19501 cacaggcatt gagtttaatt gccacaggta cagcactggc aatggtatta ggattacctc
    19561 tcgggcgcat tgtggggcag tatttcggtt ggcgaatgac cttcttcgcg attggtatcg
    19621 gggcgcttat tacccttttg tgcctgatta agttacttcc cttactgccc agtgagcatt
    19681 ccggttcatt gaaaagcctc ccgctattat tccgccgccc ggcattgatg agcatttatt
    19741 tgttaactgt ggtggttgtc accgcccatt acacggcata cagctatatt gagccttttg
    19801 tgcaaaacat tgcgggattc agcgccaact ttgccacggc attactgtta ttactcggtg
    19861 gtgcgggcat tattggcagc gtgattttcg gtaaactggg taatcaatat gcgtctgcgt
    19921 tggtaagtac ggcgattgcg ctgttgctgg tgtgcctggc actgctgcta cctgcggcga
    19981 acagtgaaat acacctcggg gtgctgagta ttttctgggg gatcgcgatg atgatcatcg
    20041 ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga tgccaccgac gtcgcgatgg
    20101 cgctattctc cgggatattt aatattggaa tcggggcggg ggcgttggta ggtaatcagg
    20161 tgagtctgca ctggtcaatg tcgatgattg gttatgtggg cacggtgcct gcttttgccg
    20221 cgttaatatg gtcaatcatt atatttcgcc gctggccagt gacactcgaa gaacagatgc
    20281 aatagttgaa aggcccattc gggccttttt taatgattcg ttttaatgat ttccaggatg
    20341 ccgttaataa taaactgtac acccatacat accagcagga accccatcaa gcgggagatc
    20401 gcttcaatgc cacccttgcc caccagccgc ataattgcgc cagagctgcg tagacttccc
    20461 cacaaaataa ccgccaccag gaaaaagatc agcggcggcg caaccatcag tacccaatca
    20521 gcgaaggttg aactctgacg cactgtggac gccgagctaa taatcatcgc aatggttccc
    20581 ggaccggcgg tacttggcat tgccagtggt acaaaagcaa tattggcgct gggttcatct
    20641 tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa tcgctttctg ttgcggaaaa
    20701 agcatccgaa aaccgataaa cgcgacgatt aagccgcctg caattcgcag accgggaatc
    20761 gaaatgccaa atgtatccat caccagttgc ccggcgtaat acgccaccat catgatggca
    20821 aagacgtaca ccgaggccat caacgactga cgattacgtt cggcaccgtt catgttgcct
    20881 gccaggccaa gaaataacgc gacagttgtt aatgggttag ctaacggcag taacaccacc
    20941 agccccaggc caattgcttt aaacaaatct aacattggtg gttgttatcc tgtgtatctg
    21001 ggttatcagc gaaaagtata aggggtaaac aaggataaag tgtcactctt tagctaacct
    21061 tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg tcaattcatt
    21121 catttgactt atacttgcct gggcaatatt atcccctgca actaattact tgccagggca
    21181 actaatgtga aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc
    21241 catatggtta atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt
    21301 accgcggcac agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt
    21361 gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga cccgtatgct ggatcgcctg
    21421 gtctgtaaag gctgggtaga aaggttgccg aacccgaatg ataagcgcgg cgtactggta
    21481 aaacttacca ccagcggcgc ggcaatatgt gaacaatgcc atcaattagt tggccaggac
    21541 ctgcatcaag aattaacaaa aaacctgacg gcggacgaag tggcaacact tgagcatttg
    21601 cttaagaaag tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga
    21661 cgctattacc attcatagca ttttggactg gatcgaggac aacctggaat caccactgtc
    21721 actggagaaa gtgtcagagc gttcgggtta ctctaaatgg cacctgcaac ggatgtttaa
    21781 aaaagaaacc ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc
    21841 gcaaaagctg aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga
    21901 gtcgcaacaa actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata
    21961 ccggatgacc aatatgcagg gcgaatcgcg ctttttacat ccattaaacc attacaacag
    22021 ctagttgaaa acgtgacaac gtcactgagg caattatgaa accgctttta tccgcaatag
    22081 cagctgcgct tattctcttt tccgcgcagg gcgttgcgga acaaacccag caaccgctcg
    22141 ttacttcctg tggcgatgtg gtggttgttc ccccatcgca ggaacaacca ccgttcgatt
    22201 taaatcacat gggtacaggc agtgacaaat cggatgcgct gggcgtgccc tattataacc
    22261 aacaagccat gtagtatatt caggccccgg catatcgggg cttgttaacc tcttaccttc
    22321 accgctttac gccactgcaa accaaataca ttgatataca gtccggccat aatgagcaca
    22381 gcccctaaga attgcagacc tgttaagcgt tcatccagca gtaacgctgc acttgccaga
    22441 ccgactaccg gcaccagtaa tgataatggt gcgacacgcc atgtttcata gcgtccaagc
    22501 agcgtacccc aaatcccgta gccaactatc gtcgctacaa atgccagata catcaacgcc
    22561 agaatggtgg tcatgtcgat agtgaccaga ctgtgaatca tttttccgga gccatcgaga
    22621 atgagcgagg cgacaaaaaa cggaattatc gggattaatg cgctccagac gaccagagac
    22681 attaccgccg gacgggatga atgagacatg atctttttgt tgaagatatt gccactcgcc
    22741 caactaaatg ccgctgccag agtcaacata aagccgagca tcgccacatg ctgaccgtta
    22801 aagctatctt cgattaacac cagtacgcca gaaatcgcta acgcaatccc cgccagttgc
    22861 ttaccgtgca gccgttcgcc aaaagtaaac gcgccaagca cgatagtaaa aaacgcctga
    22921 gcctgtaaga ccagcgaggc cagtccagcc ggcataccga agttaatggc gcagaagaga
    22981 aaggcaaact gtgcaaaact gatggttaaa ccatacccca gcagtaaatt cagcggtact
    23041 tttggtcgtg ccacgaaaaa gattgccggg aaggcgacca gcataaagcg caaaccggcc
    23101 agcatcagcg gtggcatgtt atgaagcccc actttgatga ccacaaaatt aagcccccat
    23161 acaacgacga ctagtagcgc caacacccca tcttttcgcg acattctacc gcctctgaat
    23221 ttcatctttt gtaagcaatc aacttagctg aatttacttt tctttaacag ttgattcgtt
    23281 agtcgccggt tacaacggca ttaatgcgca aataagtcgc tatacttcgg atttttgcca
    23341 tgctatttct ttacatctct aaaacaaaac ataacgaaac gcactgccgg acagacaaat
    23401 gaacttatcc ctacgacgct ctaccagcgc ccttcttgcc tcgtcgttgt tattaaccat
    23461 cggacgcggc gccacgctgc catttatgac catttacttg agtcgccagt acagcctgag
    23521 tgtcgatcta atcggttatg cgatgacaat tgcgctcact attggcgtcg tttttagcct
    23581 cggttttggt atcctggcgg ataagttcga caagaaacgc tatatgttac tggcaattac
    23641 cgccttcgcc agcggtttta ttgccattcc tttagtgaat aacgtgacgc tggtggtgct
    23701 cttttttgcc ctcattaact gcgcctattc cgtttttgct accgtgctga aagcctggtt
    23761 tgccgacaat ctttcatcca ccagcaaaac gaagatcttc tcgatcaact acaccatgct
    23821 caatattggc tggaccgtcg gcccgccact cggcacgctg ttggtcatgc agagcatcaa
    23881 tctgcccttt tggctggccg ctatctgctc tgcgtttccc atgtttttca ttcaaatttg
    23941 ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc agtgtctggt cgccgaaagt
    24001 tttattacaa gataaggcac tgttgtggtt tacctgctct ggctttctgg cttcttttgt
    24061 tagtggcgca tttgcttcat gcatttcaca atatgtgatg gtgattgctg atggtgattt
    24121 tgccgaaaag gtggtcgcgg ttgttcttcc agtgaatgct gccatggtgg ttacgttgca
    24181 atattccgtg ggccgccgcc ttaacccggc taacatccgc gcgctgatga cagcaggcac
    24241 cctctgtttc gtcatcggtc tgatcggttt tattttttcc ggcaacagcc tgctattgtg
    24301 gggtatgtca gctgcggtat ttactgtcgg tgaaataatt tatgcgccgg gcgagtatat
    24361 gttgattgac catattgcgc cgccaggaat gaaagccagc tatttttccg cccagtcatt
    24421 aggctggttg ggcgcagcgg taaatccatt agtgagtggt gttgtactta ccactctccc
    24481 gtcgtcttcg ctgtttatca ttctggcgtt agtcatcatt gccgcgtggg cgctgatgtt
    24541 aaaaggtatt aacgccagac cccagaggca accagcactc tgttgacgtt cgtgccgttc
    24601 cactactggc acggcttcgc taatcattat tctgaggcaa aggcatagcc ttcatcttcc
    24661 cagccggtta tcccgccgag catgatttta accggctttc cgagtcgagc cagtttgagt
    24721 gccgcacggt ctgcaccgtt acaatgtggc cccgcgcaat acaccacaaa tagcgtatcg
    24781 tcgggccatt cggccattct ttctgcggtc atttgcttat ggggtaaatg gagagcgcct
    24841 gggatatgcc gacgcgcgaa gtcctcttca cgtccaacca catgaagtaa aacgaaatca
    24901 tcgccaatat aagccagaga gtcatgaaca tcggcgcaat ctgtttcgac actcaagcgc
    24961 gcgagaaaat gcgcctcagc aacttctggc ttcgctgtgg taaattcagt gacataacgc
    25021 atattgcttt gtccttttgg tttgttgtgt taacaataat gtaactaatc tacctttcac
    25081 tgaccgcctc tcgatatgcc agaaatgagc aaaatgatga caacgttaca acgtccgctg
    25141 gtcattgcac tggcatatga cgggctatgt acttttgaat ttggcgttgc cgttgaaata
    25201 ttcggcctgc cacgaccgga actgggcgat aactggtatc gttttgctgt tgctgcggta
    25261 gaaccaggtg cgctacaagc caccgggggg atccacgtgg ttgctgatgg tggactggag
    25321 ttattgccca acgccgatat gattattatt cctggctggc gcggaataga tgccaaagtg
    25381 ccggagatgc tatgccagtc attgcgcgag gcacaccagc gcggtactcg cctggtatca
    25441 atatgctctg gcgtgtttgt ccttgctgct gcgggccttc ttcatcagcg aaaagcaacc
    25501 acccactggc gctacactga acaactccag caccgttacc cttcaatcag cgtaagtgac
    25561 aatgtgttat atcaggatga aggacaagtg atgacctccg ccggaagcgc cgcggggatc
    25621 gatctgtgtt tacatatcgt gcgcaaagat tttggtcatg agattgctaa taatgtcgcc
    25681 cggcgactgg tcattcagcc acatcgccag ggggatcaac cacaaaatct gacgcgccca
    25741 atggcctcac cgcgtgaaag ccagacgtta ggtgccttat tcgacttttt gcagcagaat
    25801 ctggcacaaa cacatactgt gtcttccctc gccgaacggg taaatatgag tccgcgcact
    25861 tttttaagac gttttgaggc gttaactggc atgacgcccg cccgctggct gctcaatgag
    25921 cgcttaatac gagtacagga ttatctgcaa aattcggttt ttaacatcga gaaaattgcc
    25981 gagctgacgg gttttggcaa ttcagcgtta ttgcgtcatc actttcacca acgtttttta
    26041 ttatctcctt cgcaatatcg caaaaacagc cgttccccat cacaataatc gcctgattca
    26101 gcctttgttt agataacctc tgctaatatc tttctcattg agataaaaat taaggtaagc
    26161 gaggaaacac accacaccat aaacggaggc aaataatgct gggtaatatg aatgttttta
    26221 tggccatact gggaataatt ttattttctg gttttctggc cgcgtatttc agccacaaat
    26281 gggatgacta atgaacggag ataatccctc acctaaccgg ccccttgtta cagttgtgta
    26341 caaggggcct gatttttatg acggcgaaaa aaaaccgcca gtaaaccggc ggtgaatgct
    26401 tgcatggata gatttgtgtt ttgcttttac gctaacaggc attttcctgc actgataacg
    26461 gatcgttgac acagtagcat cagttttctc aatgaatgtt aaacggagct taaactcggt
    26521 taatcacatt ttgttcgtca ataaacatgc agcgatttct tccggtttgc ttaccctcat
    26581 acattgcccg gtctgctctt ccaatgacca catccagagt ctcttcagga aatgcgcgac
    26641 tcacacctgc tgtcacggta atgttgatat gcccttcaga atgtgtgatg gcatggttat
    26701 cgactaactg gcaaattctg acacctgcac gacatgcttc ttcatcatta gccgctttga
    26761 caataatgat aaattcttcg cccccgtagc gataaaccgt ttcgtaatca cgcgtccaac
    26821 tggctaagta agttgccagg gtgcgtaata ctacatcgcc gattaaatgc ccgtaggtat
    26881 cattaaccaa tttaaatcgg tcaatatcca acaacattaa ataaagattc agaggctcag
    26941 tgttgcgtaa ctgatgatca aaggattcat caagaacccg acgccccggc aatcccgtca
    27001 aaacatccat attgctacgg atcgtcagca aataaatttt gtaatcggtt aatgccgcag
    27061 taaaagaaag caccccctcc tgaaaggcgt cgaaatgcgc gtcctgccag tgattttcaa
    27121 caatagccag cattaattcc cgaccacaat tatgcatatg ttgatgggca gaatccatta
    27181 gccgaacgta aggtaattca tcgttatcga gtggcctcag atgatcaatc caccgaccaa
    27241 actggcacag tccataagaa tggttatccg ttatttctgg cttactggca tctctcgcga
    27301 ccacgctgtg aaacatactc accagccact gatagtgggc atcgatagcc ttattgagat
    27361 ttaacaagat ggcatcaatt tccgttgtct tcttgatcat tgccactcct ttttcacagt
    27421 tccttgtgcg cgctattcta acgagagaaa agcaaaatta cgtcaatatt ttcatagaaa
    27481 tccgaagtta tgattcatct ctgagataac attgtgattt aaaa
//
LOCUS       DAZKFZ010000047        27355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000047 DAZKFZ010000000
VERSION     DAZKFZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            109..651
                     /gene="zapC"
                     /locus_tag="ACHOQW_001820"
     CDS             109..651
                     /gene="zapC"
                     /locus_tag="ACHOQW_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP9572905.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(648..1757)
                     /gene="ycbX"
                     /locus_tag="ACHOQW_001821"
     CDS             complement(648..1757)
                     /gene="ycbX"
                     /locus_tag="ACHOQW_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP9572906.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2001..4109
                     /gene="rlmKL"
                     /locus_tag="ACHOQW_001822"
     CDS             2001..4109
                     /gene="rlmKL"
                     /locus_tag="ACHOQW_001822"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP9572907.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4121..6028
                     /gene="uup"
                     /locus_tag="ACHOQW_001823"
     CDS             4121..6028
                     /gene="uup"
                     /locus_tag="ACHOQW_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9572908.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6158..7411
                     /gene="pqiA"
                     /locus_tag="ACHOQW_001824"
     CDS             6158..7411
                     /gene="pqiA"
                     /locus_tag="ACHOQW_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP9572909.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7416..9056
                     /gene="pqiB"
                     /locus_tag="ACHOQW_001825"
     CDS             7416..9056
                     /gene="pqiB"
                     /locus_tag="ACHOQW_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP9572910.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9053..9616
                     /gene="pqiC"
                     /locus_tag="ACHOQW_001826"
     CDS             9053..9616
                     /gene="pqiC"
                     /locus_tag="ACHOQW_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP9572911.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9872..10039
                     /gene="rmf"
                     /locus_tag="ACHOQW_001827"
     CDS             9872..10039
                     /gene="rmf"
                     /locus_tag="ACHOQW_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP9572912.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10109..10627)
                     /gene="fabA"
                     /locus_tag="ACHOQW_001828"
     CDS             complement(10109..10627)
                     /gene="fabA"
                     /locus_tag="ACHOQW_001828"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP9572913.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10696..12456)
                     /gene="ycbZ"
                     /locus_tag="ACHOQW_001829"
     CDS             complement(10696..12456)
                     /gene="ycbZ"
                     /locus_tag="ACHOQW_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9572914.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12642..13094
                     /gene="matP"
                     /locus_tag="ACHOQW_001830"
     CDS             12642..13094
                     /gene="matP"
                     /locus_tag="ACHOQW_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP9572915.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13170..14210)
                     /gene="ompA"
                     /locus_tag="ACHOQW_001831"
     CDS             complement(13170..14210)
                     /gene="ompA"
                     /locus_tag="ACHOQW_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP9572916.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14567..15076)
                     /gene="sulA"
                     /locus_tag="ACHOQW_001832"
     CDS             complement(14567..15076)
                     /gene="sulA"
                     /locus_tag="ACHOQW_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP9572917.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15295..15924
                     /gene="sxy"
                     /locus_tag="ACHOQW_001833"
     CDS             15295..15924
                     /gene="sxy"
                     /locus_tag="ACHOQW_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP9572918.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15887..18049)
                     /gene="yccS"
                     /locus_tag="ACHOQW_001834"
     CDS             complement(15887..18049)
                     /gene="yccS"
                     /locus_tag="ACHOQW_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP9572919.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18059..18505)
                     /gene="yccF"
                     /locus_tag="ACHOQW_001835"
     CDS             complement(18059..18505)
                     /gene="yccF"
                     /locus_tag="ACHOQW_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9572920.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18628..20682
                     /gene="helD"
                     /locus_tag="ACHOQW_001836"
     CDS             18628..20682
                     /gene="helD"
                     /locus_tag="ACHOQW_001836"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP9572921.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20714..21172)
                     /gene="mgsA"
                     /locus_tag="ACHOQW_001837"
     CDS             complement(20714..21172)
                     /gene="mgsA"
                     /locus_tag="ACHOQW_001837"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9572922.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21268..21930)
                     /gene="yccT"
                     /locus_tag="ACHOQW_001838"
     CDS             complement(21268..21930)
                     /gene="yccT"
                     /locus_tag="ACHOQW_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP9572923.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22103..22516
                     /gene="yccU"
                     /locus_tag="ACHOQW_001839"
     CDS             22103..22516
                     /gene="yccU"
                     /locus_tag="ACHOQW_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9572924.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22561..22878)
                     /gene="hspQ"
                     /locus_tag="ACHOQW_001840"
     CDS             complement(22561..22878)
                     /gene="hspQ"
                     /locus_tag="ACHOQW_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP9572925.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22936..24126)
                     /gene="rlmI"
                     /locus_tag="ACHOQW_001841"
     CDS             complement(22936..24126)
                     /gene="rlmI"
                     /locus_tag="ACHOQW_001841"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP9572926.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24221..24499
                     /gene="yccX"
                     /locus_tag="ACHOQW_001842"
     CDS             24221..24499
                     /gene="yccX"
                     /locus_tag="ACHOQW_001842"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9572927.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24496..24825)
                     /gene="tusE"
                     /locus_tag="ACHOQW_001843"
     CDS             complement(24496..24825)
                     /gene="tusE"
                     /locus_tag="ACHOQW_001843"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP9572928.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24916..25575)
                     /gene="yccA"
                     /locus_tag="ACHOQW_001844"
     CDS             complement(24916..25575)
                     /gene="yccA"
                     /locus_tag="ACHOQW_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP9572929.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25983..27002)
                     /locus_tag="ACHOQW_001845"
     CDS             complement(25983..27002)
                     /locus_tag="ACHOQW_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9572930.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARPGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26980..27222)
                     /locus_tag="ACHOQW_001846"
     CDS             complement(26980..27222)
                     /locus_tag="ACHOQW_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9572931.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         6940 a         6654 c         7145 g         6616 t
ORIGIN      
        1 ttcattgctg ttgcttattt agacattttg tacttttatt gttgaggtta ttaagcgaag
       61 cgacaatgga ttgtgttgct gcggttttat agggtagggg agaggcagat gcgaattaaa
      121 ccagacgata actggcgttg gtattacgat gaagagcacg atcgtatgat gctcgattta
      181 gccaatggta tgctatttcg ctcacgtttt gcgcgcaaaa tgttgactcc agatgccttt
      241 tctcccgcag gcttttgcgt tgacgacgcc gcgctctatt tctcttttga agaaaagtgc
      301 cgcgatttta atttatctaa agaacaaaaa gccgaactgg tgctgaatgc actggtagca
      361 attcgctatc tcaaaccgca aatgccgaaa agctggcatt ttgtttccca tggtgaaatg
      421 tgggtgccca tgccgggcga tgcggcctgc gtttggttga gcgacactaa cgagcaagtg
      481 aatttactgg tcgttgaatc tggcgagaat gccgcgctat gcctgctggc acaaccctgc
      541 gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca ttaaaatcat gaacgacagg
      601 ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac aggcagtcta acgcgccaac
      661 ttaagtgcag tcttcggtac acagctacag caaagaatgg tgccatcatc gcccattgct
      721 gattttttca gcggcgtgac ttcgccttct aaaagctgaa cacggcaact tccacaaatg
      781 cccgcgcggc aagaataagg gatacgaatt ccctgatttt ctaattgctc cagcaacacc
      841 tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa tatctacatt ggcgtccggt
      901 tgttgcgtga tgttggcagt atcatcagcg gcagctgcgc cgtaaatttt tgccggagcc
      961 gttgtcagaa tttccacctc atcgccaacg cgaatcacgc cgctattacg ggcaattaaa
     1021 ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc ggaaagattg caatgttttt
     1081 aatggttcgc ctgccggatg tttttgccct ttttctgggc tgacggtggt gaaaatacag
     1141 cggctacaag gtttaaccac atcaaacacc acatcaccaa tgcgaatcac tttccaacta
     1201 tcttcttccc aggctgacgc gccggaaacc accagattgg ggcggaattg ctccattttt
     1261 acactggccg gacaacgttg ttggagatca cgtaacgagg cttcgttagc aagaaggtaa
     1321 ggatagccat cagcaaatga caggggtaca gtgttgtggc gtttcacgcg ccgggtcatt
     1381 tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa atccacttaa ccatttgttg
     1441 atcgcttctg gcgcaattcg cgcggtaaaa tgtgtgcccc aaacttcggt tggtgcgtct
     1501 tgtgtggcga aatcagcaaa acgaacatat gcgctactgc tatctggtgc ggtgagatgc
     1561 aagccatcat gcacgggtga aggagtaaat cgtaccatct ggggaaactg gcgagcggtg
     1621 ataaacgtac cgtcaggttc cgtgatcata aagatgcgat cgaaggccag accactgaca
     1681 tctgccagag catgtgtaag accaatgccg cgcatcgatt taacaggatg aataaaaagc
     1741 cgggttaatg tcgccacggt gcggtcctca aatgaaaata agccctcaac tttatgacat
     1801 gacgcgctta ttagctataa tgcgcaacaa ttttcttagc gcctgtccca ttaggctatt
     1861 ttatttgtca gtttggccct gggttgtgct cgaaatcctc acgtacttgt gtacgctccg
     1921 gtttctccgc gctgtccatg tccagactga ctgcaacaat tacgcctact gcggtaggct
     1981 cttagagtaa aagtgacgat atgaattctc tgtttgccag tacggcccgt gggctggaag
     2041 agctgttaaa aactgaactg gaaaacctgg gggccgttga atgccaggtg gttcagggtg
     2101 gggtccattt caagggcgac acacggcttg tttaccagag cctgatgtgg agccgcctgg
     2161 cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta cagcgattta gacctctatc
     2221 tcggtgttca ggcgatcaac tggacagaga tgtttaatcc tggcgcgacc ttcgctgtcc
     2281 acttcagtgg tttgaatgac actatccgca acagtcagta cggcgcgatg aaagtgaaag
     2341 acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg tccaaatgtt gatcgcgatg
     2401 cgccggatat ccgcgttaac gtctggctgc ataaagaaac cgccagtatc gcccttgatc
     2461 tcagtggtga tggtttacat ctgcgtggct atcgcgatcg tgctggtatt gcgccgatca
     2521 aagaaaccct ggcagccgct attgtgatgc gatccggctg gcagccagga acaccgttgc
     2581 tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc ggcgatgctg gcgaccgatc
     2641 gcgcaccagg cttgcaccgt gggcgttggg gctttagcgg ctgggcgcag catgatgaag
     2701 ctatctggca ggaagtgaaa gcggaagcgc aaactcgcgc ccgtaaaggc ctggctgagt
     2761 atagctctca tttttacggt tcggacagcg acgctcgggt gattcaacgt gcacgcacta
     2821 acgcccgtct tgcggggatt ggtgaactga tcacctttga ggtgaaagat gtcgcgcaac
     2881 tgaccaatcc gctgccgaaa gagccgtacg gcacagtgtt aagcaacccg ccatacgggg
     2941 aacgtctgga cagcgaaccg gcgctgattg cgctgcatag cctgctgggt cggatcatga
     3001 aaaaccagtt tggtggctgg aatctctctt tgtttagtgc ctcgccggat ctgctaagct
     3061 gcttgcagct gcgtgcagac aaacagtaca aggcgaaaaa cggcccgctg gactgcgtac
     3121 agaaaaatta ccacgttgcc gaaagcactc cagacagcaa accggcgatg gtagcggaag
     3181 actacgccaa ccgcctgcgt aagaacctca aaaaattcga gaagtgggct cgtcaggaag
     3241 ggattgaatg ttaccgcctg tatgacgccg atctgccgga atataacgtt gccgttgacc
     3301 gttatgccga ctgggtggtg gtgcaggagt atgcgccgcc aaaaactatt gatgcgcaca
     3361 aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat ttcggtactg gggattgcgc
     3421 caaacaaact ggtgctgaaa acccgtgaac gtcagaaggg caaaaatcag taccagaaac
     3481 tgggcgagaa gggcgagttt ctggaagtta ccgaatataa cgctcacttg tgggtgaacc
     3541 tgacggatta tctcgatact ggtctgttcc tcgatcaccg tatcgcccgt cgtatgctcg
     3601 gccagatgag caaaggcaaa gatttcctca acctgttctc ttataccggc agcgccaccg
     3661 tgcatgcggg attaggcggt gcacgcagca ccaccaccgt ggatatgtcg cgtacttatc
     3721 tggagtgggc agaacgcaac ctgcgtctga atggcctaac cgggcgtgcg catcgcctga
     3781 ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga acagttcgat ctgatcttta
     3841 tcgatccgcc aaccttctct aactcaaaac gaatggaaga tgcgtttgat gttcagcgcg
     3901 atcatctggc gctgatgaaa gatttgaaac gtctgctgcg tgcaggtggg acgatcatgt
     3961 tctcgaacaa caaacgtggc ttccgtatgg atctcgacgg cctggcaaaa ctgggactga
     4021 aagcacaaga aattacgcaa aaaacgctct cccaggattt cgcccgtaac cgccagatcc
     4081 acaactgctg gctgattacc gcagcctgaa aggaatagta atgtcattaa tcagtatgca
     4141 tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat aacgcagaac tgcatatcga
     4201 agataacgaa cgtgtttgtc tggtgggccg caacggcgca ggcaaatcga cgttaatgaa
     4261 aatcctcaac cgtgaacaag ggctggatga cggtcgcatt atttacgagc aggatttgat
     4321 tgtagcgcgt ctgcaacagg atccgccgcg taacgttgag ggtagtgttt atgatttcgt
     4381 tgccgaaggc attgaagaac aagcggaata cctgaaacgc tatcacgata tttcgcgcct
     4441 ggtgatgaac gacccgagcg agaaaaatct caacgaactg gcgaaggttc aggaacagct
     4501 ggatcaccac aacctgtggc agctggaaaa ccgcatcaac gaagtgctgg cgcaactggg
     4561 gttagatcct aacgttgcgc tgtcgtcgct ttccggcggc tggttgcgta aagcggcatt
     4621 aggacgcgcg ctggtgagca atccgcgcgt attgctgctg gacgaaccga ctaaccacct
     4681 ggatatcgaa accatcgact ggctggaagg gttcctcaaa accttcaatg ggacgattat
     4741 tttcatctcc cacgaccgtt cgtttatccg caatatggcg acgcgcattg ttgatctcga
     4801 tcgcggcaag ctggtgacct atccgggcaa ttacgatcag tacctgctgg aaaaagaaga
     4861 agccctgcgc gtggaagaac tacaaaatgc cgagtttgat cgcaaactgg cgcaggaaga
     4921 agtgtggatt cgccagggga tcaaagcacg ccgtacccgt aatgaaggcc gcgtacgcgc
     4981 cctgaaagcg atgcgtcgcg agcgtggcga acgtcgcgaa gtgatgggta ccgcaaagat
     5041 gcaggtggaa gaggccagcc gctccggtaa gatcgttttc gaaatggaag acgtttgcta
     5101 ccaggttgac ggaaagcaac tggtgaaaga tttttctgcc caggttctac gtggcgacaa
     5161 aattgccctg attgggccga atggctgcgg caaaaccacg ctgctgaaac tgatgctcgg
     5221 tcagcttcaa gcggacagcg ggcgtattca cgttggcacc aaactggaag tggcttattt
     5281 cgatcagcac cgcgcggaac tggatcccga taaaacggtg atggataacc ttgccgaagg
     5341 taagcaagag gtgatggtta acggcaagcc acgccacgtt ttgggctatt tgcaggactt
     5401 cctgttccat ccgaaacggg cgatgacgcc agtacgtgcg ctttctggcg gtgagcggaa
     5461 ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac ttattgattc ttgacgaacc
     5521 gaccaacgat cttgatgtcg aaacgctgga actgctggaa gaactgatcg acagctacca
     5581 gggcacggta ttgctggtaa gccacgatcg tcagtttgtc gataacaccg ttacagaatg
     5641 ctggatcttc gaaggcggcg gtaaaattgg tcgttatgtc ggcggttatc atgatgcccg
     5701 tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg gtgaaaaaaa acgaagaacc
     5761 cgccgcgcca aaagcagaaa ctgtaaaacg cagcagtagc aaactaagct ataaattgca
     5821 gcgcgaactg gagcagctac cgcaattgct cgaagatctg gaggcgaagc tggaagccct
     5881 acagacgcag gtggcggatg cttccttctt cagtcagccg catgagcaga cacaaaaagt
     5941 gctcgccgat atggctgctg cagagcagga gctggagcaa gcctttgaac gctgggagta
     6001 tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt cgatttaaac agagacagct
     6061 gacaacggta aaattgcctg atgaactggg tttatcaggc atgaaaacaa acactttgtt
     6121 agtcatctgt aaaatagcgc atcattaagg agtaccaatg tgcgaacatc atcatgccgc
     6181 gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg ttaccgcgcc ttgagcatgg
     6241 tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc gtggcgtggg atgccccccg
     6301 gcagcgtccg accgcctatg cgttggctgc actgttcatg ctgttgctgt ccaacttgtt
     6361 tccttttgtg aatatgaacg ttgcaggagt taccagtgaa attacattac tggaaattcc
     6421 cggcgtgctt ttttctgagg actacgccag cctcggcacc tttttcctgt tgtttgtgca
     6481 actggttccc gcgttttgtc tgataaccat tctgttactg gtgaatcgcg cggaattacc
     6541 ggtccgttta aaagagcaac tggcacgggt gctttttcaa ctcaaaacct ggggaatggc
     6601 ggagattttc ctcgcgggtg tgctggtcag tttcgttaaa ctgatggctt acggcagcat
     6661 tggcgtaggc agcagctttc tcccctggtg tttattttgt gtcctgcaac tgcgcgcttt
     6721 tcagtgcgtt gatcgtcgct ggttatggga cgatatcgcc ccgatgccag aactgcgcca
     6781 gccgctaaaa ccaggcgtca cggggatacg tcaggggctg cgttcgtgct cctgttgtac
     6841 ggcaatcctt cctgctgatg aacccgtgtg cccgcgctgt ggtactaaag ggtacgttcg
     6901 acgtagaaac agcctgcaat ggacactcgc gctgcttgtt acgtccatca tgctgtatct
     6961 tccggctaat attttgccca tcatggtgac ggatttatta ggctcgaaga tgccatcgac
     7021 gattctcgct ggggtcatcc tgttatggag cgaaggctct tatcccgtcg ctgcggtgat
     7081 ctttctggcc agtattatgg tgccaacgtt aaagatgatc gccatcgcgt ggctgtgttg
     7141 ggatgccaaa gggcatggca agcgcgacag tgaaagaatg catttgattt atgaagttgt
     7201 tgagtttgta ggccgctggt cgatgattga cgttttcgtt atcgcggtgc tctcggcgct
     7261 ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg ggtgcattaa tgtttgcttt
     7321 agtcgtcata atgacaatgt tttctgctat gacgtttgac ccgcgtttgt cgtgggatcg
     7381 tcaacctgaa tcagagcatg aggagtcctg acagtatgga atctaataat ggggaagcca
     7441 aaatccagaa agtgaagaac tggtctcccg tgtggatatt tcctatcgtc acggcgctca
     7501 ttggggcctg ggttcttttt tatcattaca gccatcaggg accggaagtg accctgatca
     7561 ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat taaaagccgt agcgttgacg
     7621 tcggcgtggt tgaaagcgcc acactggctg atgatttgac acacgttgaa atcaaagcgc
     7681 ggctgaattc cggtatggaa aaattgctgc ataaagatac cgtcttttgg gtggtgaaac
     7741 cgcagattgg tcgagaaggg attagcggtc tgggaaccct gctgtctggg gtttatatcg
     7801 aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata cgatttgctg gactcgccac
     7861 cgttggcccc gcctgatgcg aaaggtatcc gtgtggttct cgatagcaaa aaagccgggc
     7921 agctctcgcc aggagatccg gtgctgttcc gtggctatcg ggtaggttcg gttgaaacca
     7981 gcaccttcga tacgcaaaaa cgcaatatca gttatcaact gttcatcaat gcaccttatg
     8041 accgactggt gaccagcaat gttcgcttct ggaaagatag tggcattgcg gttgatctga
     8101 cgtcagcggg aatgcgtgtg gagatgggct cattgacaac gctgctgagt ggcggtgtca
     8161 gctttgatgt gccggaaggt ctggatttag ggcagccagt ggcaccgaaa acagctttcg
     8221 ttttgtatga tgatcagaag agcattcagg attcgttgta caccgatcac attgattatc
     8281 tgatgttctt taaagattcg gtacgcggtc tgcaaccggg agctccggta gaattccggg
     8341 gtattcgcct cggtaccgta agcaaagtgc cattctttgc gccgaatatg cgtcagacat
     8401 ttaacgatga ttaccgtatt ccggtgctga ttcgtatcga gccagagcgg ctgaaaatgc
     8461 agcttggcga aaatgcggat gttgttgagc accttggcga attgttgaaa cgtggtttac
     8521 gcggatcgct gaaaaccgga aacctggtca ctggcgcact gtatgttgat ctcgatttct
     8581 atccaaatac gcctgcaata accggtattc gtgaatttaa tggttatcag attatcccga
     8641 ccgttagcgg cggcctggcg caaatccagc aacgactgat ggaagcgttg gataagatca
     8701 acaaactgcc attgaatccg atgattgaac aggcaaccag tacgctttct gaaagtcagc
     8761 gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa caagatcctc gccagccagt
     8821 cgatgcagca gttgccgacg gatatgcagt caacgttgcg tgaattgaat cgcagcatgc
     8881 agggcttcca gcccggctcc gcagcctaca acaagatggt ggcggatatg cagcgccttg
     8941 atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct caatgagaag agtaacgcgc
     9001 tggtatttga agcgaaggac aaaaaagatc cagagccgaa gagggcgaaa caatgaaaaa
     9061 gtggctagtg acgattgcag cactgtggct ggccggatgc agctccggcg aaattaataa
     9121 aaactattac cagttacctg tggtgcagag cggtacacaa agtaccgcca gccagggcaa
     9181 tcgcctgtta tgggtagagc aggtcgctgt acctgactat ctggcgggga atggtgtggt
     9241 ttatcaaacc agtgatgtga agtatgtgat tgccaacaac aacttgtggg ccagcccgtt
     9301 ggatcaacag ttgcgcaaca ccctggtagc caacctgagc acgcaactgc ccggctgggt
     9361 ggttgcctcc cagcctctgg gaagcgccca ggacacgctc aatgttaccg taacggagtt
     9421 taacggtcgc tatgatggca aggtcattgt cagtggtgag tggctgttga accaccaggg
     9481 acaactgatc aaacgtccgt tccgtctgga aggagtgcaa actcaggatg gttacgatga
     9541 gatggttaaa gtgctggccg gtgtctggag tcaggaagcc gcttctattg cacaagagat
     9601 aaagcgtcta ccttaattat aaagatttgt aaatataacc gtctccggta tgttgcctga
     9661 ggcggttttt ttgtttctaa cgtgcggaaa aatttgttcc tcttcacatt ttttgtacaa
     9721 ccgacatgcc cgtgtagctc acaaatatga cagtggcgtg aattttgcgc attgacggca
     9781 gttatgattc gcggtattgc ttaactgtga ttgcacattt agtaatcact gttttctttt
     9841 ccaccagaaa ccagtatgag ggaaacgagg catgaagaga caaaaacgag atcgcctgga
     9901 acgggcacat caacgtggtt atcaggccgg catcgccgga cgctcaaaag aaatgtgtcc
     9961 ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc tggcgagaag ccatggcgga
    10021 cagggtagta atggcctgat tctgtctctt taaaaagaaa cctccgcatt gcggaggttt
    10081 cgccttttga tactctgtct gattataatc agaaggcaga cgtatcctgg aacagaccga
    10141 ctttcaggtc gctggcggta tagatcagac gaccatcaac cagcacttcg ccatccgcca
    10201 ggcccataat cagacgacgg ttaacaatgc gtttaaagtg aatacggtag gtcacttttt
    10261 tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc aacgcccagc gcgcggcctt
    10321 taccttcgcc gcccagccag ccgaggtaga accctaccag ctgccacatt gcgtccaggc
    10381 ccaggcatcc cggcataacc ggatcgccaa taaagtggca tccgaagaac cacagatccg
    10441 gattgatatc cagttctgct tcaacatacc ctttgtcgaa gttaccaccc gtttcggtca
    10501 ttttgaccac acggtccatc atcagcatgt tcggtgctgg caattgcggg cctttagcgc
    10561 caaacagttc accgcgacca gaggcaagaa ggtcttcttt tgtataggat tcgcgtttat
    10621 ctaccatgtt ctctgtaagc cttattttat tgaagcacgc aggatagcta acacgtgtac
    10681 gctgaacaag tccgatcagt tcggaataaa ccagttcagc caacgtaatg gccatggaaa
    10741 acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt tcctggatgg tttgcatcag
    10801 cgtcgtttgg ccttcgccat cccacaccag atttaataat aacggcagtg cgtcagtcac
    10861 atcgtctact gcccagatgg tgaatttgcc ttcttctacc gctttcacca gttcactgtg
    10921 aagacttaaa tggcgaacgt tagctgtggg gataatgaca ccttgtttcc cggttaactc
    10981 acgttgctgg caaatagcaa agaagccttc gattttctca tttaaaccac cgaccggctg
    11041 ggcgcgaccg aactgatcga ctgaacctgt gatagcgata ctctgattca ccggcacatc
    11101 ggcgagggcg cttatcaggg cgcagagttc agccatcgag gcactatctc catcaacttc
    11161 actgtatgac tgctcaaatg tcagcgatgc tgagaagggg atctgttgct caagctgtag
    11221 ttccgacatc aggaacgctt gcatgatcat catccctttc gcatggatat tgccgccaag
    11281 ctccgctttg cgttcgatgt cggtgaattc accatcgcca atatgcacaa cgcagctaat
    11341 gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat tcaatgaccg aaagggcgtt
    11401 aatttgcccg atgcgttcgc cttcggtttc aatcaggatt tgctcctgaa ggatctcatc
    11461 ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc tgctgcagca ttaagtttag
    11521 ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg acctctttac actggcggag
    11581 gatccactgc gggctaagcg gaagtgtttc ttgttcaccg gtgtagcgtg ctgcttcgcg
    11641 gataagtacc ggccaggcat ccgctcccgg ggcaggtaag tgattatgtc tggcggtaaa
    11701 tgtcacccag cgacaccact gggttactga ctccgcatcg acaatctgca gagtatcttc
    11761 aaattcgcta taaatagcct gctctgaaag ctctggctcc atctcctgga aatcagccaa
    11821 tgattcgcgt tcgcctacca gaatgacttt cagcttcaat ggcatcgaag gcacagagac
    11881 ggggagaggg cgcgactcat caaacgcaac ccagtcaaaa cgctcgcggt taacgatatt
    11941 tttcagccgc atccacagca gaggttgcgc cagcagtgta cgcaaagaga tgatgagaat
    12001 accgccattt gcctgatgca ccagaccagg ctgcagggta atgtcgccat taaactggcg
    12061 caggcagcca aagagttgct ccgcttctac ccagtcggca gcgacaactt gcgttaaagt
    12121 cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg atggagtggc cggaaacctc
    12181 atagtgaccg cccaccagtt ggcctgcatc gctttgtagc gtacgcgcgg cattggcaat
    12241 aagattcaga tactcagact cttccggggc cttcgccagc ataaaagagg aggatgctcg
    12301 cgtatgcagc aactgctcca gcgcaaattg cagccgcggt tgagtatcac tgaataaagg
    12361 atcgttttcg tcaatcaaat gtggctgagc aaatatttcc tgatagctat cggtatcagg
    12421 aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt gatgtttttt agtctgttgt
    12481 caaagccgcg attataccgt aaccggcact acagcacacg tagaaagcac cgacaatact
    12541 cctggcatgg gcgttaaagc tcacaggatg gagattcttt tcttcactgg cctaaaaagc
    12601 tgatattctg taaagagtta cacggtaaca ttgagatcgc tatgaaatat caacaacttg
    12661 aaaatcttga aagcggttgg aaatggaagt atctggtgaa gaagcatcgc gaaggggagt
    12721 taatcacccg ttacatagaa gccagtgccg cccaggaagc cgtcgatgtg ttgctctcgc
    12781 tggaaaatga acccgtgctg gtaaatggct ggattgacaa gcatatgaat ccggaactgg
    12841 tcaatcgcat gaagcaaacc attcgggcaa gacgaaagcg ccattttaat gcggaacacc
    12901 agcatacgcg caaaaaatcg atcgatctgg aatttattgt ctggcaacgt ctggctggtc
    12961 ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt tcagctgatt gaagatgcgg
    13021 aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa gcaggatctg caggcattgc
    13081 tgggtaagga ataactgacg aaagtcagtt caatttacta aaggcaaaaa aaaccccgca
    13141 gcagcggggt ttttctacca gacgagaact taagcttgcg gctgagttac aacgtctttg
    13201 atacctttaa cttcgatctc tacgcgacga tccggagcca ggcagtcgat cagtgcagca
    13261 cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt
    13321 gcggagatct tgtctgccgg gatacctttg gagatcaggt aatcaacaac agactgagca
    13381 cggcgctcgg acagaccctg gttgtaagcg tcagaaccga tgcggtcggt gtaacccaga
    13441 acaactacgg aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga
    13501 gcagcctgac cttccggttt cagggttgct ttgttgaagt tgaacagaac gtcagacttc
    13561 agagtgaagt gcttggtctg tacttccggt gccggagctg gagccggagc aactactgga
    13621 gctgcttcgc cctgaccgaa acggtaggaa acacccaggc tcagcatgcc gttgtccgga
    13681 cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc actggtattc cagacgggta
    13741 gcgatttcag gagtgatcgc gtactcaaca ccgccagcga agaccggaga aacgccggtg
    13801 tcgtggtttt taccataaac gttggattta gtgtctgcac gccataccat accacccaga
    13861 cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca gtttagcggt cagttgaacg
    13921 ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt acggcatacg acctaaccag
    13981 tcgtaaccca tttcaaagcc aacatacggg ttaacctggt aaccaccaaa agcaccagcg
    14041 cccagttggt tttcatgggt cgggccattg ttgttgatga aaccagtgtc atggtactgg
    14101 gaccagccca gtttagcacc agtgtaccag gtgttatctt tcggagcggc ctgcgctacg
    14161 gtagcgaaac cagccagtgc cactgcaatc gcgatagctg tctttttcat tttttgcgcc
    14221 tcgttatcat ccaaaatacg ccatgaatat ctccaacgag ataacacggt taaatccttc
    14281 accgggggat ctgctcaata ttaactctac cgatatcttc ggcttatgcc gagcacccct
    14341 ggcgatgtaa agtctacaac gtagttgaaa acttacaagt gtgaactccg tcaggcatat
    14401 gaaaaaaaag tcttgtataa ggtatgttta atcttttttg tcagcgacaa tttacagaag
    14461 agaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa aattagtcac gactgaaagc
    14521 attggctggg cgacaaaaaa agttccagga ttaatcctaa atttacttaa tgatacaaat
    14581 tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg agaggatgcg cttaccggac
    14641 gcataataaa ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt
    14701 cttcagtcaa atcatctgcc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag
    14761 cgcgaaccat tgactccaca gtgtggcaag gggagagctg gctaatctgc attactttcg
    14821 ttaagggtag cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc
    14881 agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg
    14941 tcatcatggg ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt
    15001 ccgtagagac acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat
    15061 agcctgaagt gtacataatc aatccagccc ctgtgagtta ctgtatggat gtacagtaca
    15121 tccagtgaca acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg
    15181 tgacgggaaa acataaatta atcttgccgc ttaagaataa gttgcctatt ttcgtagtta
    15241 acggatccgt taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa
    15301 agcctctcct ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt
    15361 gaataccgat cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg
    15421 gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa
    15481 tatccgcctg tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat
    15541 cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct
    15601 ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg
    15661 cccaatatgt cttttcatct ggaagcgatt ctcggggagg tggggattaa ggatgtacgg
    15721 gcgttacgta tacttggggc aaaaatgtgt tggttgcgac tgcggcagca aaacagtctg
    15781 gtgacagaaa agattctgtt tatgcttgaa ggtgccatta tcggcattca tgaagctgcg
    15841 ctcccggtgg cacgccgcca ggagcttgca gaatgggctg actctcttac gccgaaacag
    15901 gagtttcctg cggaacttga gtaatctggc gttgcagacg accaatctca ggcagtaatg
    15961 caatcaataa tccaacttgt tgtacgacca gaggttcttt gctgtctgcc cgtggttcaa
    16021 gttgctgcat ccgctgtttc aggctagcta atgcctcatt gacgcgttct tcatcagcag
    16081 gttgatgatg taacgcgtca tcaacatagc aaactgcgtc atcaagaaac gccagaattt
    16141 caggattagt taactgctcc cggtgagcac cgagggctga gatatagctg gtaaacgtat
    16201 ggttaaggca cagcaaccga aacgcggctt cgcgaatttg cggggtaacg ttcggctcgc
    16261 tggacatatt tgataccacc gacgccagct cagcatcacg gttgtgtgca tcgcggcggg
    16321 caatacgata cgccagacgg ttatcacgcc cctgatggta ttgctccagt atggcatcga
    16381 gataccgaca gttggcctct gtggcgcgtt cgagcatgcg cggcagattg cgaaactgcc
    16441 agtcaggcca gatgtagctc actgccgccc acgcaatggc acaaccaatc agcgtatcga
    16501 ttacgcgagg taacgctact tcaaaacctt cacccagtaa gttaaaacac agtagcacca
    16561 aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt acggaaggca aaaaagagca
    16621 cgccggtaat aaccagcagc accagctgcc cttccagtga tggcacaaac cacagcacag
    16681 gaatgccaat ggcgatacct accagcgtac caataatcct taacttcagg cggtggcgcg
    16741 tggcgttata gtttggctgg cagacaaaca aacttgtcag caagatccaa tacccgtgat
    16801 gcattccggt tatctgaatg atggcgtagc cgaagcacaa caccagcgac attcttaccg
    16861 catgacggaa gagggcggat tccggcgtga agtgacggct aagacgcagc cagatatcac
    16921 tcaacccgtg cgggctgtca tcagcgagct cattttcgtc attattatgg ggtagtgcct
    16981 gggcctgttc tgattcaatt gttgccagtt gggcatcaat ggcgcgtaaa ttgttcagca
    17041 aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt atcgcgcatc cgctccagcg
    17101 cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc atgttgataa ggctgacgca
    17161 acaaaataca gcgtgacagt tgctggcacg cctggccctg catcgacatc agccgctgaa
    17221 aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa tgtttgatac tgaatatgag
    17281 aagagctggc acgctcgtga atatcctgtg cgacaaagta ataatgcagc gtgcgacgcg
    17341 ttccccgttg accacgatcg ccacgtaagc gggtcagcag cgagagtttc gtctgattca
    17401 atgtcgccat cagcagaccg ttggcgagag ccaaatcgta cagcggtgcc tggctttcat
    17461 cttcaatatc aggatcaaac atgcgcgact tgagctcaag ataacgcgcc agttgttcat
    17521 agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa cagcagatga ccaataagtg
    17581 tcaggacgtt gtaccagacg gcaccggcca gcagatacat cggctgctga taccagtgct
    17641 catacagtga tgttcccaac atagtgtaaa tggcgatcag caatgcaccg aaggcaattg
    17701 ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc gctggtagag agcgttaagc
    17761 caatcgcaaa tagccagggc cagggaaaca gcaattctac tgaggccgag gcgataaaaa
    17821 agcagaacag cgtaatgatg aggttacgca aacgtcccgc cagtcggtca tcgagatcgg
    17881 tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt cagttttaca tcacccagcc
    17941 accacggaaa cgctgtggtt ccacaaagcg caataaaaat acgcgcgtaa tacagccagg
    18001 cgctgttcca ggtatagcgt ttgagcaaag gacttagcat aaaggccata ttagtggatt
    18061 attcaaaacg acgacgtgca ttggcttcac gcgcagcttg cgctgtttct accgatacca
    18121 cgcgacgacc aaccggccat agtgcaatag cggcaatttt aaagttcgca atgccgacag
    18181 gaatgccaat gattgaaata cattgtgcga tgcccgttgc aatgtgcatc aggcataacc
    18241 accagccaaa gaaaatcagc cagaaaatat tcaataccgt accgccagta ttcagcagca
    18301 catttttgcc agccgggttc agttcatcga catgaatagc ttcattgcca taaggcacca
    18361 gagacagttt agtgatctcc cagcaggatc gtgtcagcgg taaggtaaaa atcagcgcaa
    18421 tactgaccag agtcgccaac agccagccca gagtggtggc aaatccgcca agcacaaagt
    18481 tcagaatgtt caaaacggta cgcataaaac ctcgctttac tgtggttttc agtaatgggc
    18541 ggcaattgta acgttttttt gggctggagc acgttttctc tgacggttac actgataaga
    18601 aataatttcg tgtggatcta cagagtcatg gaactgaaag cgacaacgct tggaaaacgt
    18661 ctggcacagc acccttacga tcgggcggtg atcctcaatg ccgggattaa agtctccggc
    18721 gatcgccacg aataccttat tcctttcaat caattactgg cgattcactg taagcgcggt
    18781 ctggtatggg gcgagctgga atttgtactg ccggacgaaa aagtggtgcg tctgcacggt
    18841 accgaatggg gcgagacgca gcgtttttac catcatcttg atgctcactg gcggcggtgg
    18901 agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc agcaactgga tttgattgcc
    18961 acgcgcactg gagaaaataa atggctgacg cgtgagcaaa cctctggcgt tcagcaacaa
    19021 atccgccagg ctttgtcggc gttgccgttg ccggttaacc gactggaaga attcgataac
    19081 tgccgtgagg cgtggcgtaa atgtcaggcc tggttgaaag atattgaaag cgctcggttg
    19141 cagcataacc aggcgtatac cgaagccatg cttaccgagt atgcggattt tttccgccag
    19201 gtcgagtctt caccgctgaa tccggcgcag gcccgggcag tcgttaatgg cgagcattct
    19261 ctgttagtgc tggcaggtgc aggaagcgga aaaacgtcgg tgctggtggc ccgtgcaggc
    19321 tggttgctgg cgcgtggtga agcgtcccct gagcaaattt tattgctggc gtttggtcgc
    19381 aaagccgctg aagagatgga cgagcgtatt cgcgaacggc tacataccga agacattacc
    19441 gcacgcacgt ttcatgcgct ggcgctgcat attattcagc agggcagcaa aaaagttccg
    19501 atagtcagca aactggaaaa tgataccgct gcccgtcatg aactctttat tgctgagtgg
    19561 cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct ggcggcaatg gctgacggaa
    19621 gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg atgaaaaatt acagcgtcgt
    19681 cttgcctcac gcctcgatcg ttgggtaagt ctgatgcgga tgcacggtgg tgcacaggca
    19741 gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt tcagtaaacg tatcaagttg
    19801 atggccccgt tattaaaagc ctggaaaggt gcgctgaagg cagaaaacgc tgtcgatttt
    19861 tcgggcctta ttcatcaggc gattgtgatt ctggagaaag gtcgctttat cagcccgtgg
    19921 aagcatattc tggttgatga atttcaggat atctcgccgc agcgggcagc gttgttagcg
    19981 gcattacgca agcaaaacag tcagacgacg ttgttcgccg ttggtgatga ctggcaggcg
    20041 atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg ctttccatga aaactttggt
    20101 gaaggcgaac gctgtgattt agacacgact taccgtttta acagtcgtat cggtgaggtg
    20161 gcaaaccggt ttattcagca gaacccaggc cagctgaaaa agccgctaaa cagcttaacc
    20221 aatggagaca aaaaagccgt cacgttattg gatgagagtc aacttgacgc tttgctggat
    20281 aagctctctg gttatgccaa accggaagag cgcattctga tcctggcgcg ttaccatcac
    20341 atgaggcctg ccagcctgga aaaagcggca acacgctggc cgaagttgca aatcgacttt
    20401 atgaccattc atgccagcaa agggcaacag gcggattacg tcctcatcgt tggcttgcag
    20461 gagggaagtg atggttttcc ggctgcggcg cgggagtcga ttatggaaga ggcgctactg
    20521 ccaccggttg aggatttccc ggacgctgaa gaacggcggt taatgtacgt ggcgctgacc
    20581 cgggcacgtc atcgggtatg ggcactgttt aacaaagaga atccctctcc ctttgtggaa
    20641 atactgaaaa atctggatgt gccggtggcg agaaaaccgt aagaaacagg tggcgtttgc
    20701 cacctgtgca atattacttc agacggtccg cgagataacg ctgataatcg gggatcagaa
    20761 tatcgaccgc gtcgttgaaa tgcggcgact ggataatgaa gtctgccgtt gccacgttgg
    20821 tggcgaccgg aatgttccat accgtcgcca gacgcagcaa ggctttcacg tcaggatcgt
    20881 gcggcacggc atttagtgga tcccagaaga aaatcaatac atcaattttc ccttctgaga
    20941 tcaatgcgcc aacctgctgg tcacccccca ttgggccact caacatcgcg ttgacgttca
    21001 tgccggtcgc gcgggaaatt aagttaccgg tagtgcctgt tgcatacagt acgtgttgtt
    21061 ccagtaacgg ttgatgccgt tccacccagc tcatcagcat ttgtttgcag tgatcgtgtg
    21121 ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt cgtcagttcc ataatgtaca
    21181 tccgtagtta actttcctac agattactgt aagcacttat cgctgcaaga taaagaccga
    21241 aaaagcctgc gcacaggcac aaaaatctca ggaagatggt tgtttttccg cccattgcag
    21301 gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa tacttcagcc gttgttctgt
    21361 gagcacctga gactgcggtg gaatagcatc cagctcggaa acacccgaaa gcagcgtact
    21421 gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg cgtttcgcgg atttgttata
    21481 gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt gaggtaatgg cgagaatatc
    21541 cagctttacc ggaatcggcg tcgcatcgcc atccaacaat tcaaggcgcg gcgcggcatc
    21601 aaaatggtta gcttcccgct cattctccag gcgaggcagg cgaaaattga cctggttgat
    21661 gagctgggta ttaaaactga ccaccagcgg tggggagata tacagccgtt cttcactatt
    21721 ggaaagatga atcgtcttct caacgcgaaa cactaactga tgcggtccat tatccagttc
    21781 aatgctgtcc gcaccgcgca gcagagagct ggaaaccttc ttgccgtcga gtaccagaag
    21841 atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg gataccggca aacataatgc
    21901 aatcaaggtg gtcacgatgc cggttttcat aggtagctcc tgtcaaaaga ccgcccgaat
    21961 aataatgtgt caaaattttt ctttaatcat acttactaac atatagacat atttcccgct
    22021 tttgctctca ttcattcgta ttagctgcat ggttggcatg tcggctttgt cgtacacttt
    22081 gtgaaacagt ctggaggaaa taatgaaaga aaccgatatt gcaggcattt taacgtccac
    22141 tcacaccatt gcgctggtgg gggcaagcga caaacccgat cgtcccagct atcgcgtgat
    22201 gaaatatcta ctcgaccagg ggtatcacgt cattccggtt tcgccaaaag ttgccggcaa
    22261 aacgctgctg ggccagcagg ggtacggcac gctggcagac gttccggaaa aagtcgatat
    22321 ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgta gcgcaggaag ctatcgccat
    22381 tggcgcgaaa accctctgga tgcaactggg ggtgattaat gagcaagcgg cagtactggc
    22441 acgggatgcc gggttgaatg tggtgatgga tcgctgcccg gctattgaga tccctcgcct
    22501 gggcctggcc aaataaaaaa tccccggaag gcaaaaacct tccggggatc tgttcaggga
    22561 ttagttacgc agacgcgggg cctggagttg tttgcggatg gtctgcgcca gctcatccat
    22621 ggatggctgt tcaggatgtt catcctgcaa ttcgctactt agctgggctt cagccaggta
    22681 ggtatgaacc ggtaggccgt tatcgtcctc catcaccacg tgataccacg gagcggcgcg
    22741 aagctcgtca ttcaccgcca gctcatcagg cgacggttca gagagcgaat aaaccgggtc
    22801 gatatccacg accactccga gataacctaa cagggaatgg cggacctgct ggccgatacc
    22861 gaatttgctg gcaatcatag tcacctcccg ggaaatcttt acctttgata taagggtaaa
    22921 attccacatt tcaagttaca tgacgcgaca ggcaaaccct ttcagatata gcccttccgg
    22981 ataggtagcg atcaccggat gatcggccgc ctgacggaac tgctctataa attgtacatc
    23041 acgaccggca tcaattgcgg catccgcgat gattttctga aataaatcgc tggtcatcag
    23101 accggaacag gagaaagtca ggagaatacc gccttcattc agcagctgaa tcgccagcat
    23161 gttgatatct ttatagccac gacacgcgcc catcaactgg cttttattct caacaaactt
    23221 cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga tcgcgatagg tacgcagcaa
    23281 tttaaagaca tcatcacgga caaactcagc cttgctcaga tccagtttgt tcagctcaac
    23341 gttctgccgt gcaatatcca gtgcttcctg ggaggtatca acgctgacaa cctggctgca
    23401 accgcccatc agtgccgata cggcgaaacc accggtatag gagaaacagt tcagtacgcg
    23461 tttattttca acgtagcggc gggtagccag gcggctgtct cgctggtcca ggtagtagcc
    23521 cgttttgtgt ccgtgctgta tgtccaccag cagcttcatg ccatgttctt caatcggcag
    23581 cagggcaggc ggcaactcac cggtgacgag gccctgggtc agctccatcc cttctttttt
    23641 acgtaccgca acatcgctgc gatcgtaaat cgcacattcc gggtacagcg tttgcagggc
    23701 actaattaat gccgcgcgct gatattctgc gccagcactc agcagttgca gcaccagaaa
    23761 attaccgaaa cgatcgatag taatacccgg caggccatca gattctccgg cgattaaacg
    23821 atagctgtcg aggccatctt tttgcgccag ccagtcacgc catttttgtg cttgttgcaa
    23881 acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg tcaaacgtcc agacgcgcgc
    23941 ccggatttgc gaagctggcg aataagcgcc gcgtgctaac cattttccct gatgatcaac
    24001 aatatcgatg gtttcaccga ggctggcttt accttccata cgggcaacgg ccccggaaaa
    24061 gacccacgga tggcgacgaa gtaatgattt ttcgcgccct ttggctaaca ctaaacgtac
    24121 actcataatt tacttttctg tcgatgcaaa gaaatgggcg tcattgtccg gagttttcca
    24181 tggatttgca acgcgtccat taaggataag gagagcagca atgtcgaaag tctgcataat
    24241 tgcctgggtt tacgggcgag ttcagggcgt aggatttcgc tacaccacac agtacgaagc
    24301 gaaaagactg ggcttaaccg ggtacgccaa aaatcttgac gatggcagcg tggaagtggt
    24361 tgcctgcggt gaagaagggc aggtagaaaa attaatgcag tggttaaaga gtggcggccc
    24421 gcgttctgcg cgtgtagaac gggtgctcag cgagccgcat catccctcgg gggaattaac
    24481 cgattttcgt atccgttaaa tacattttac cggttttggc aggccagcaa ttttggtggc
    24541 ttgcttagcc ggacctttcg gaaacagtcg gtataaatag cggctgttac ctttttcttc
    24601 gccaaattta ttcgccatcg cttttaccag catacgaatc gccggagaag tattgaattc
    24661 cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt tctggcgaca gcgaaatccc
    24721 ttcgttctct gcaatcacca ctgccagcgg ctcactccac tggctgcttt ctttgagata
    24781 gccttcggta tccgtttcta tctctttacc ttcgaagatc agcataatta ttactacctt
    24841 aatcagactg ccggcagtgt aacaaaaaac aaagccccgc ataaagcgag gctatgaaag
    24901 tgttagcggg tgagattaat cgcggctggc gaagcccaga atgctcagca ggctgacgaa
    24961 gatgttgtac agcgaaacat acaggctaac cgtggcacga atatagttcg tttcaccgcc
    25021 atgaatgatg ttgctggttt caaacaaaat agcgccagag gagatcaaaa tgaagaccgc
    25081 gctgatcgcc agatgcagag caggcagctg caggaagata ttagcaacca taccaatcag
    25141 caccaccaca atacccgcca tcagcatacc gccgaggaac gacatatctt tgcgggtggt
    25201 cagcacatat gcagagcagc agaagaacac taacgccgtt ccgcccagtg ccatagcgat
    25261 tacgtcaccc attccggcag acagataggt attcagaatt ggtccgagga tataacccag
    25321 aaaaccggta aaggcgaatg cggagataat cccggtcggc ttattcgcca ttttataggt
    25381 caggaacatc aaaccataca tacccaccag cgtcagaatc agacccggag atggcagcat
    25441 cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc agcgtcaggc tcagcagaaa
    25501 ataggtatta cgcagcactt tatgggtgct aagcagtgat gtacggtcat gtgaagaact
    25561 aacaatacga tccattgagt cactctctat gacagatgta attaattaag cagcataatg
    25621 ataatgcgta agggcaccca gtagttttac ccatctttac gcatttgatc tggaacaggt
    25681 ttaacagcgg attatcaggt cattaagcaa atataacgcc ctgagaattt cggcaggcaa
    25741 aagaaaaagg ggttagcatt tagctaaccc cttatcttat ctggcggaag cgcagagatt
    25801 cgaactctgg aaccctttcg ggtcgccggt tttcaagacc gaagaaaaac aaataaaaat
    25861 cagtgtaata cgtgaaattg ctgaaaagtt aatcaaattt aacaccaaga aaaatcaagg
    25921 tattaggaag ttgctcatag ctagtatacc aagtcttttt gccagaaatt tttctggatt
    25981 gtctatttgc taaccacgag cggcaaatcc agtgttggtg tgatttttgt tttacgatcg
    26041 taaatcaaca cctgattttc tgttttatgc ccgctgaaaa tttgtttgtc gcgactgctg
    26101 ccttcgtaat ctgagatccc cttggctttt atgtcgtgaa aattgcaccc gaacggaacg
    26161 ccaacttttt gctcggctgc gcgtttagcc tgattccacc agttattcag cgttttagct
    26221 atgactttcc cgcctttggt tgtattgatc acatattcgc atgtacagga agatacattt
    26281 cgggctaact ggatcgctgt acgtaatcgc ggtgaccatt ccttgatttg tttggtgccg
    26341 gttttgtttt gctcaatgta gatcccttta tccataatat cctgccattt caactcaagc
    26401 acatcaccgg gtcttgccgc acagagatag gaaatctcca ttgcaatgcg taactgtgga
    26461 attgcttccg catatatcgc cgcatactgt tcatcggtga tgtaaacagt gcgggcttta
    26521 agagagaatt ttctgactcc tttgcatggg ttattcttca catacccacg ctcatatccc
    26581 cacccgtata ctcgactcag gcttgccagt tcatgatttg cctgggtctt gctctcaagc
    26641 cctcgtttat ccatgaaaat tcttacctgc tcagttttga cattatcagc aagcactttt
    26701 ccgaatacca tcagcaacgc cttctgatgt tgtcgataat ctttttgggt tcggggggcc
    26761 agttctgtaa atgcagggga gtccataaac atgtgccaca atttagctac ggtcattatg
    26821 ttgtggagtt ttgctttttc cagttcataa ttttgccaga ctttagctac gctggttttc
    26881 cgcactcttc ctagccctat acttcttgta cttccttcgg gtttccacac gtaactgtaa
    26941 ccattcgatc taacccgcgg tggcagtaca ttatcttttt tattttttct tggtcttccc
    27001 attgttcagc gcctcaaaat cgggttcagc agaaaccagt tcagatgttt ttggcatcgt
    27061 tgtcagaccg tgtggaatat ccctgcggag aactatgggt tcgtttttag ggccgattac
    27121 aaatgggatg ccgtgcagtc ttaactggtg ttgctgtttt gtgtatcgct cgtatttcgt
    27181 gatctcttta atctctgctg gagatagagt taattcgtac atgtggtcac gtgcctttac
    27241 agcatgaccg ccgccactat agctggtggg cggcgatatg ggttgaacat caaaaatcag
    27301 cctgactcgg gagcagtttt tgccagatgg ctgaaacgta tttcgcctgg taacg
//
LOCUS       DAZKFZ010000048        27270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000048 DAZKFZ010000000
VERSION     DAZKFZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            31..1047
                     /locus_tag="ACHOQW_001847"
     CDS             31..1047
                     /locus_tag="ACHOQW_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9572932.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            1132..1554
                     /locus_tag="ACHOQW_001848"
     CDS             1132..1554
                     /locus_tag="ACHOQW_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9572933.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1551..1754
                     /locus_tag="ACHOQW_001849"
     CDS             1551..1754
                     /locus_tag="ACHOQW_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572934.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1758..2165)
                     /gene="cueR"
                     /locus_tag="ACHOQW_001850"
     CDS             complement(1758..2165)
                     /gene="cueR"
                     /locus_tag="ACHOQW_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP9572935.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2162..3337)
                     /locus_tag="ACHOQW_001851"
     CDS             complement(2162..3337)
                     /locus_tag="ACHOQW_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP9572936.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3334..5496)
                     /locus_tag="ACHOQW_001852"
     CDS             complement(3334..5496)
                     /locus_tag="ACHOQW_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP9572937.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5500..21237)
                     /locus_tag="ACHOQW_001853"
     CDS             complement(5500..21237)
                     /locus_tag="ACHOQW_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9572938.1"
                     /translation="MDINTIATDDIINASEKGADLQISGTSNQPAGTTITLALNGQNY
                     TATTDAAGNWSTTVPASAVGALGEASYTVTANVTDSAGNSNSASHNVQVNTALPGVTI
                     NPVATDDIINAAESGNAQTISGQVTGAAAGDTVTVTLGGKTYTATVQGNLSWSVDVPA
                     ADIQAIGNGNLTVNASVTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVVNSIEHAQ
                     ALVITGSSSGLAAGAALTVVINTVTYAATVLADGTWSVGVPAADVSNWPAGTVNITVS
                     GTNTAGTTSTITHPVTVDLAAVAISINTVSGDDVINAAEKGADLTLSGSTSGVEVGQT
                     VTVTFGGKTYTATVAGDGSWTTTVPAADLSVLRDGDATVQASVSTINGNTASATHAYS
                     VDATAPTLAINTIATDDILNAAEAGNPLTISGSSTAEAGQTVTVTLNGVTYSGSVQAD
                     GSWSVSLPTADLSNLTASQYTVSASVSDKAGNPASANHGLAVDLTVPVLTINTVSGDD
                     IINAAEHGQALVISGSSTGGEAGDVITVTLNSKTYTTMLDASGNWSVGVPAADVTALG
                     SGPQTITAAITDAAGNSDDASRTVTVNLAAPTIGINTIATDDVIKATEKGADLQITGT
                     SNQPAGTTITVTLNGQNYTATTDSNGNWSATVPASAVSALGEANYTVTANVTDTAGNS
                     NSASHNVLVNSALPAVTINAVATDDIINAAESGNAQTISGQVTGAAQGDTVTVTLGGN
                     TYTATVQSNLSWSVDVPAADIQALGNGDLTVNASVTNGVGNTGSGSRDITIDANLPGL
                     RVDTVAGDDVINSIEHNQALVITGSSSGLTAGTALTVEINNVTYGATVLADGTWSLGV
                     PAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVTVDLAGVAITINTLSGDDVINAVEK
                     GETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEANGSWTVNVPPADLAALPDGAGNVQ
                     ASVSNINGNSAQADRAYSVDATAPLVTINTIASDDILNVSEAGAGITISGTTTAQAGQ
                     TLTVTLNNNTYQTTVLADGTWSVNVPAADLSGLTASSYTVTATVSDKAGNPASADHAL
                     VVDITAPDLTINTVAGDDIINAIEHGQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLD
                     ASGNWSVGIPAADVTALATGSQTITASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSG
                     DDIINAAEIVVAQTISGQVTGTAVAGNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQ
                     ALGNGELTISASLTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQALVI
                     TGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPVADVTNWPAGTVNIAVSGTNT
                     AGTTTSISHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVEAGQTITVI
                     FGGKSYTTTVAADNTWGLTIPAVDVATLPDGAANVQASVSNVAGNSTQATHAYSVDAT
                     APSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADGSWS
                     VSVPTGDLASLTASSYTVNASVSDKARNSASATHNLTVDLAAPVVTINTVAGDDIINA
                     TEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQGDV
                     TITATVTDSAGNSGTASHTVTVALGAPVLAINTIAVDDIINAAEKGADLAITGTSNQP
                     AGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDADGNSGSAS
                     HNVQVNTALPGVTINVVATDDIINAAEAGVEQTISGQVTGAAAGDTVTVTLGGATYTA
                     TVQANLSWSVDVPASALQELGNGELTISASVTNSVGNTGNGTREITIDANLPGLRVDT
                     VAGDDVVNIIEHGQALVITGSSSGLAAGSNVTLTINGQTYVAAVLADGTWSVGVPAVD
                     VSAWPAGSVTIAASGSTSAGNPVSVTHPVTVDLSAVAVSINAITADDVINAAEKGAAL
                     TLSGSTSGVEAGQTVTVTFGGKTYSATVAANGSWSTSVPAADMAALRDGDASAQASVS
                     NVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQTVTV
                     TLNGTNYTGTVQTDGSWSVSVPSADLSTLTASNYTVNAAVSDKAGNPASVNHNLTVDT
                     SVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDASGN
                     WSVGVPASVVSALANGTVTINASVTDAGGNSGSATHQVTVNTGLPTITFNAISGDNIL
                     NADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAALGQA
                     NYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGADQTISGVVT
                     RAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASVTNANGNTG
                     SGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVLTVTINSVA
                     YSATVQADGSWSVGIPAANVSAWPAGPLTVEVDGQSSANNPVSVSHPFTVDLTAVAIS
                     INTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAANGSWSVNVP
                     AADLATLPEGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAAEAG
                     SPLTISGTSTAETGQTVTVTLNGATYTGTVQADGSWSVSVPTSALGALNASNYTVSAT
                     VNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSSGGEAGDV
                     VSVVLNGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNSDDASRTVT
                     VSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQNYSATTDAS
                     GNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGITINPVATDD
                     IINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQALG
                     NGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHAQAQVITGS
                     SSGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITVSGANSAGT
                     QTSITHPLTVDLTAVAISMNSITSDDAINAAEKGAALTLSGSTSGVEAGQTVTVTFGG
                     KTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYSVDSAAPT
                     VTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQTDGSWSLTL
                     PASDLTALANNGYTLTATVSDLAGNLGSASKGVTVDTTAPVISFNTVAGDDVINNVEH
                     IQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTVTIS
                     ATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGTGTNFATG
                     TVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSAGNSATASR
                     SVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYTATV
                     AANGTWALNVPAVDLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAPTVTIATVA
                     GDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVFIPAQQFAG
                     LSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEHGSSLVIS
                     GTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVINASVSNAI
                     GNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLTAALASNE
                     TAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGATDSQNVVI
                     DTTAPDPAVKTIAISAITTDMGLITNDFVTSDTTLAVSGTLGATLSAGEFAQISLDGG
                     VTWTTLTVVGTSWSYADGHTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAK
                     SITITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTVAA
                     DSLNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATSSQSALIDTVNPAQVLTIASISTD
                     TGSSATDFITSDTMLTLTGSLGAGLASGEVAQISLDSGATWTTLTTNGTQWTYTDSRT
                     LTDGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQ
                     ATDDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYS
                     ARVVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVE
                     VVINGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNA
                     NISNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWS
                     KTALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQV
                     TVGTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSA
                     TVGGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNT
                     TNTFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPV
                     AVGATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGGSQS
                     GTTSGVAAMDYDWDGAVDVLVSKQSGSVFLSRNTNTVSYGTSLHLRITDPNGINVYYG
                     NTVKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASN
                     IDQTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSG
                     GWVYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSN
                     FNDILTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVG
                     TWEGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTI
                     VQIDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            complement(21274..25659)
                     /locus_tag="ACHOQW_001854"
     CDS             complement(21274..25659)
                     /locus_tag="ACHOQW_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9572939.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(25762..27117)
                     /locus_tag="ACHOQW_001855"
     CDS             complement(25762..27117)
                     /locus_tag="ACHOQW_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFP9572940.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT         5083 a         8025 c         7905 g         6257 t
ORIGIN      
        1 atacgactta ttttgaacaa gagaaaatga atgaaaactg taaacgtagc tttactggca
       61 ctcataattt cagcaacatc cagccctgtt gttttagctg gtgataccat tgaagcggcg
      121 gcaacagagc tttcagccat taactctggc atgtcgcaat cggagattga gcagaagatt
      181 acccgctttt tagaacgcac agacaacagc cccgctgcgt atacctattt gactgaacat
      241 cactacatcc cttctgaaac acctgatacc actcagactc ccactgtcca gacagatcct
      301 gacgcaggac aaaaaaccgt tgccgctaca ggtgatgtac agacaactgc ccgttatcag
      361 agcatgatca acgcccgaca gtctgcggta actgacgccc agcaaacgca aattacagag
      421 caacaggcgc agatcgtagc cacacaaaaa acgctcgccg cgactggaga tacgcaaaat
      481 accgcgcatt atcaggaaat gattaatgcc agactggcgg ctcaaaatga ggctaatcag
      541 cgcaccgcca ctgaacaagg gcagaaaatg aatgcgctga caaccgatgt ggcagtacaa
      601 cagcaaaatg aaaggactca atacgataaa caaatgcaaa gtctggcgca ggagtctgcc
      661 caggcacatg aacaaattga cagcctgtca caagacgtaa cccaaacgca ccaacagtta
      721 accaacaccc aaaaacgggt tgcagataac agccagcaaa ttaacacgct caataaccat
      781 ttcagttcgc taaaaaacga agttgatgac aatcgtaaag aagccaatgc gggaactgca
      841 tctgccatcg ctatcgcctc acaaccacag gttaaaaccg gtgacgtgat gatggtgtca
      901 gcgggagcgg gaaccttcaa cggtgaatct gcggtgtctg tcggaacatc atttaatgcc
      961 ggaacgcata cggtacttaa agccggtatt tctgcggata cacaatctga tttcggcgca
     1021 ggtgtcggcg tgggatattc gttctaatat ttcaatcctc aatataaata agagcaagga
     1081 agcttgccgg gttcatctct tcattaattt gtacattatt taaggttaac aatgatgaat
     1141 agctccatta aatcgttttc cctgctggcg gttatattac tggctggctg tagttcaccc
     1201 acttcccgca tcgcagattg ccaggcgcag ggcgtcagtc atgacacctg ttacctcgca
     1261 gaacagcagc gtcaggcggc tattttaagt gcatccgagg cacaggcatt taaaaatgca
     1321 gaagccgcac aacacgccca ggcggcaaag aaagccattt ataaaggatt tggcatgacc
     1381 tttagaatga gcagtaaaaa ctttgcttat ctcaatgatt cattatgtgc aattgatgaa
     1441 gacaataaag atgccactgt ttatcagtca ggtctatata acgtcattgt ttatcatcac
     1501 acaggaaaag tcgccttaat gaaagaaggc cagtttgtgg gttatttaaa atgaaggagc
     1561 aaaggaaaat acccctgacg catattatga ttatcggtgc gtttattttt gccttcttgc
     1621 aagtagtatt attagcctcc ctggttcacg ctgtgaatgt taacaacgaa atccaggaag
     1681 gcttatttca gtcggggcgc attatggtag aaagtttgca gcatattctt tcggtgcaaa
     1741 cggggattca ctgattttca ccccgcccga tgatgacagc agccggagag attttcgata
     1801 atcgggcagt cggcgctgtc atcaccgggg caggcattcg ccagcgccag tagctggttg
     1861 cgcatagatt gcagttcttc gatatgccgt tcaatctccg ccaccttctc cagcgtgcga
     1921 cgtttgacgt cggcactgtg acgctgcggg tcgttaaaca gattcaccag ctcgccgctc
     1981 tcttccaggt taaagcccac ctggcgcgcc tggcgcagta aggtcaattc gttgagatgc
     2041 tgctgcgtgt aggttcgata accattttcg ctgcgcatcg gcggcgtcac cagccccttc
     2101 tcttcataga agcgaatggc tttgctggtc aggccggtaa tttttgctac atcgctaatg
     2161 ttcatcgttc gcgcaacgcc tcctgtgctt tgttaaaggg tttcaacaga taatccagca
     2221 cgcttttttt gccggtgcgg atatcgaccg tcgccaccat accggggaat atcggaaact
     2281 gctgtttgct tttgttttcc agatggttgc tgaaagtgcg aatgtacacc cgatagtaat
     2341 aaacatcgcg ccgcacttca tcctgcacgg tgtctggtga tatcaccgcc acttctcccg
     2401 gcaggccgcc gtagatggag taatcgtagg cagttatttt caccagcgat ttttgccccg
     2461 ggtggataaa cgccacatcg cgcggggaga tttttgcctc aatcagcagt tgctcatcca
     2521 gcggcacgat ggtcatcagt ttaccaccgg gggcgatgac gccgccaacc gtggtgacat
     2581 caatatcctg aacaatgcct cgcaccggcg cggtaaagtt gagcctggtg agagagtctt
     2641 cccgtccacg gatcaccgaa cgctgggttt ccacttccgc attggctttt gctaactctt
     2701 cgcgcgcctg aacgtagtat tgcgtgcgca cgtcgctgag tttgttttcc agttcattgg
     2761 cctggcgttg caggcgcagt acttcgacgc tgctcgctgc cccctgtttt accagcggct
     2821 gggtcatcgc cagttcgcga ttgaccaggc taatcccctg cgtcagcccg gtggtggctt
     2881 tgttcaaccc gtcgcggcga gtgttgtaca acgccgtttc cgactcgata agttcgggga
     2941 atttattcag ttcgtcaggg aagaccagcg gtttattgct cacttccgcc cgcagccgcg
     3001 ccgaggttgc cagcgccgcg tacagccgcg acatcgcttc ctggacgtcg gattccgttt
     3061 tggtgcggtt aagctgcgcc agcggctggc cctgttcgac aatatcgcca actttcacgt
     3121 cgagatgata cagaattccg ccttccagcg actgcaccac ctgttcgcgc ccggaaggtt
     3181 ctaccgtgcc gctgccggtg gtgacttcat caagctggaa gtaccacgcc cagccgagaa
     3241 aacagagcag catcacagcg gtaacgcgta ccgccagcgc cgaacgcggc aggcggggtt
     3301 cagtcagacc acggttatat tgcagttgtc cgttcatgcc gcagacccct tattcttctc
     3361 ctgcaacacc acccgtcgcg ctgtgggctc gccctgttca cgcaaaatgg cctctttcgc
     3421 gccgtccatc acaatccgcc cctgatccat cacgattatc ctatccacca gttgcagcat
     3481 cgccgtgcgg tgagtggcga taatcagcgt gcgatgccca atccactgtt tcagttgttc
     3541 gattaaatac gcttctgcca tttcgtccag tgaggcgctg ggttcgtcga gcaggacgat
     3601 attcggctga gagatgagta gccgcgccag cagcagcgtt tgccgctgac caccagagag
     3661 gccaaatccc ccttcctgaa tcatgtagtt gaggccgttt ttctgcttct gcacaaaggg
     3721 cagcgctccg ctgagggtta acgcgcgttc gatatcttca tcgctggctt ccggcatccc
     3781 catggtgagg ttttcccgca gcgaaccaaa gaacagtcgc ccggtctggc tgagtagccc
     3841 catgtctcgc cgcagatctg ccggatcaag ctgcccgata ctgatgttat ccagcaacac
     3901 ctgcccctgc tgggcgatac gcattccggc gagcatttgc agtagcgtcg atttacccgc
     3961 gccgttgcgc ccgagaatgg cgattttctc accagcctga atgttgagtt tgccaatcgc
     4021 tacatcggca atcttctctt cttcgtcgta ataaaacacc gcgttgctga actgataatt
     4081 accgtgcagc accgctttat gcaccagttt gccgtgttcc ggctgatcca ccgggcgctt
     4141 catcagttca tcaaggccgt tacgtgctac tttcgcctgc tgccagcgcg agagcacgcc
     4201 ggatatctgc gacagcggcg caatggtgcg cgaagccagg atcgtcgtcc cgaccagcgc
     4261 gccggtggtc atgtcgccgt tcatcaccat aaagcagccc accagcagca ccaccacgta
     4321 gacgatggac tgcacttcct gggtccaggt gagcagcagc ccggtgagaa aacgttgctt
     4381 catgctgatg gacgaggcca cgtcgttggt gtggttccac tgattctgaa aacgctgttc
     4441 ggcgcgcagc agtttaatat cttcaatcga ctgcacggct tccaccagcg tagcgttgcg
     4501 taccgctgac tcgcgcattc cttcgttcgc cagccgcgcc agcgggcgtt gcaccagcag
     4561 gccggggatc accagcaacg gcagcgccag caaaaccacc agcaccagcc agccgccgat
     4621 catccacaaa ataaagacaa acagcaggaa gaatggcagg tccgccaccg cgccgatggt
     4681 ggtggacgta ataagctccc gcactgattc cagttcgcgg atctgcgaga taaacgatcc
     4741 ggtggatttc gatcgcacgt tgtttttcag ccgtagcgca tgaccaaaaa cgcgatccga
     4801 aatacgcagg tctgcccgct ttccgatcac atcagataga tgcgtacgca ccatgcgcat
     4861 acagaactca aacaggatcg ccatcatcac cccggcgaaa agcacccata gcgtcgggta
     4921 cgattgcgcc ggaaccaccc gatcgtaaac ctgcatcgag aaaatcatcg ccgccagcgc
     4981 cagcacgttc gccaccagtg acgccagcat aatatcgccg tagcgccgcc agtcttttag
     5041 cgaaagactc cagaaccagt tcgcctgata cgggcgaata tagtcatcca cccgcgcgtc
     5101 agggatggcg ctgagagggc gcagcagcgc cagttcgacg attttatctt caatatccgc
     5161 gacgttaagt gtagtttcca gcccttcgtc accgctgaac tgaacgctga tattgtcatg
     5221 attgtcgcga cgggtgatca cgccaatctg ctgattatca aacaccgcga tcagcggcag
     5281 acgccagggg ttaagcgaat cggcggaaaa ctccaccaac cgcaacccca tccctaactg
     5341 ccgcgccatg tcggtgagta actcctcccg cttactgtcg cgctcccagt tgacggtcgc
     5401 gcgcacattc tcctgagaaa aatcgagccg ataatagcgc gccacggcga tcatcgcctc
     5461 cagccaggct tccacggact gggcattttt tttcattatc tacaccacca tcaactgatg
     5521 attcgccagc aaggtggcga ggtcggtgtg cacgcccgtc agcgtgacca cgttagttgt
     5581 cgcaaaagtg ccgcccgtgc cgtcgcggtc gatctgcacg atggtgtcgc tgccgctctg
     5641 ggtgactttg acaaagtcac cgatgtttcc ggcctgcgca tccagcgttg ccacgccgtt
     5701 gacgtagctg gctttgccgt tgccggtgta gccgctgccc tgtaacagtt cacgaatatc
     5761 aatgcgatcg gtgtcggcgg tgccttccca tgtccctacc gtaaagccat tcaccacgtc
     5821 tgagccgttg ccgccagtgg cgtcgctggc gttgagcagt ttatagagca aggtgtcgtg
     5881 gccgccgttg ccgatgttga gcgtgtcgtt gccgccgcgc ccttccagtt ggttatcgcc
     5941 gctgctgccg gtcagaatgt cgttaaaatt cgagccggaa atgccttcga tattggtaaa
     6001 tgtcgaggtg ttaaaaccgg tggcctgcgc ggcagtgctg cttaagttag ccgtcacgcc
     6061 caccgtcgaa agccggaaat caaccacgtc catcccgccg ttcgccagcc acgttccggt
     6121 gccggagctg tacacccagc cgccggaacc gtcgtaagtg tcggtgcctg cggtggcgaa
     6181 gaaggtgtcg ttatagccgg tgccgacgaa cttgccgttg ttgctcgcgg tacccgcttc
     6241 agcgctgaga tcgtaagcgt gagtggcatc ggtggcctgt aaaccgcccc agctggtgtt
     6301 gaccgtctgg tcgatattgc tggcggtggt gccggtggat ttgatcagca ccgcgttgta
     6361 ggtttctccg gcattcagcc cgtagaagtt gaccagcgcc gaggtgtcgt taacccccat
     6421 acccgactgc gggttgatga tttgcgtggc gaccagcact cccgccgagt tgtacagctt
     6481 cacggtattg ccgtaataga cgttaatgcc gttgggatcg gtgatgcgca ggtgtagcga
     6541 agtgccgtag ctcaccgtgt tggtgttgcg gctcaggaac acgctgcccg actgtttgga
     6601 caccagcaca tccaccgcgc cgtcccagtc gtagtccatt gccgccacgc cgctggtggt
     6661 accgctctgg ctgccgccga gggccgattg cgtccagttg ctggcgttgc tgacgttagt
     6721 ataaagatac gactgcccgg tctgggcgat acgggcgata tccatcaggc cgtcgtggtt
     6781 ccagtccacc gccaggctaa actggctggc gtaggtggtt gccgttgcgc ccaccgccac
     6841 cgggctaccc agtacgccgt tggtgttgaa taacagcgag ccgtagttag ctcttccctg
     6901 gctggcgggc aggaagagat ccatatcgcc gtcaccatcg aaatcggccc aggtcatgga
     6961 aacgctgctg ctcatagcgc cactgcccgc cccgctcagg aaggtattgg tggtgttctg
     7021 cccccagaca aacgtcccat tcccctggtt gatgagcgaa gacagcgtgt aatagttgtt
     7081 caggttatag gtgtgctgaa ccaggtcgat cctgccgtca ttgttcagat cgacgccaga
     7141 accttcgttg agcgaaagat accccgtcac cgccccgccc accgtggcgc taccgccgct
     7201 gttcgacgtg gtggagttgc ctaccagcgt gcctgcgttg ttccacagga aggtgttgga
     7261 gtccggcccg ccagcgtcac caatccagaa gtcgagatag ccgtcgccct ctttatcaaa
     7321 tgccacaatc gagccgtacc acgtcagggt gccgacggtg acctgaatag cgctggaaaa
     7381 ggtgccatcg ccgttattgg taaagccgtt aatatagccc gtaccgtagt catcacgggt
     7441 gataaacaga tcgcccgtgc cgttacggtc gtaatcggca atggagctgg tggcgtagtt
     7501 gttgccgctc tgatacagcg tcagcgcggt cttcgaccag gtgagtgggt cagtcgattg
     7561 cattacctgc tggtttgcca gcaccgtcca catcccgtgc gagtcgaggc cgtaggtcag
     7621 cccccaggcg gtggttttgc tggcggtagt ccaggtcggt gtgtaatcaa tcgccgcgtt
     7681 aaccgtcacc gtgccgttgc taatattggc gttattgccg ttacccgccg aactttttac
     7741 ctgcgcagta acggtatagg cggtcgcgga aactgtcagc gcatcggtat ccggcaactg
     7801 tacataccag gtgttgtgcg ccggatcgac cactaccgcg ccgcccggtt cagaggtgta
     7861 ggttttgccg ttgatcacca cttcaacata ctgcccactg gcaagcgcgg cggtgatcac
     7921 cccgctaata atcggcgtag tatcgcgcgt ggtctggctg gtgatttgcg ccagcgtggt
     7981 cggaatagag gtatcgaccg tcaggacaaa atcactggag gaggtgatat tccccgccaa
     8041 atccaccact cgcgcgctgt aggtataggc accgctcacc agcccgctgt cgctgtaggt
     8101 ccagttcaga gcgccgacca tcgtcaccgc ccctaacagc agcccgttac ggtagaggta
     8161 aaccacttca ccgctggcaa gcggcgcgga aagtacaccg ttcagcagcg gcgtagtgtc
     8221 gtcggtggcc tgcgaactgc ttaatgtccc ctgccgctgc ccgacatcat cggtatacga
     8281 cacaatcgtt ggtgtggcgg tggggttaat cgtatcgacc accaccgttt tcgacaccac
     8341 cgggccggtg ttccccgcca gatccagcac ccgcacctga taaacgtagc tgccgtcggt
     8401 cagcgtgcgg ctgtcggtgt aagtccactg tgtaccgttg gtggtgagcg ttgtccaggt
     8461 cgcgccgcta tcaaggctaa tctgcgccac ttcgccgctg gcaagccccg cccccagcga
     8521 accggtcagc gtgagcatgg tgtcgctggt gataaagtca gttgccgaac tccccgtgtc
     8581 ggtgctgatg ctggcgatgg tgagcacctg cgccgggtta acggtatcga tcagcgccga
     8641 ctggctgctc gttgcgccaa cgttgcccgc cagatcgacc acccgcacct gccaggtggt
     8701 ggtgccgttg gtgagggttc gtccgtcaac gtaactccag ttcaggctgt ctgcggcgac
     8761 ggtcacgttc acccaggttg cgccgttgtc ggtactgatt tgcgcgaact cattagcgcc
     8821 gagcgccgcg cccaatacgc cgcgcacggt aagcgtggtg tcgctggtaa taaaatcgct
     8881 gctgctggtt ccggtgtcat cgctgatacc ggtaatggtg atacttttcg ccgcttccgg
     8941 gctggtggtg tcgaccacca cgttttgcgt tgcggtctgc ccaacgttcc ccgccagatc
     9001 caccacccgc accgtgtagt tccaggtgcc gtcagtaagc gtgtgaccat cggcatagct
     9061 ccagctggtg ccaacgacgg ttaacgtagt ccaggtgacg ccgccgtcga ggctgatttg
     9121 cgcgaactca ccggcagaaa gcgtcgcccc cagcgtgccg ctcaccgcaa gcgtcgtgtc
     9181 gctggtgaca aaatcgttag tgatcaagcc catatcggtg gtgatcgcgc tgatggcgat
     9241 ggttttcacc gcgggatctg gcgcggtagt gtcgatcacc acattctggc tgtcggtcgc
     9301 gccaacgttg cccgctgcgt caatcacccg cacctgatag agatagttgc catcggtcag
     9361 cgtgcggctg tcgttatagc gccaggtcgt gccggtaacg gtaagcgtgg tccaggttgt
     9421 gccgccgtcg atacttattt gcgccgtctc gttactggca agcgcggcgg tgagcgagcc
     9481 gttgaccact accggactga cgctggtgat aaagtcgctg gcgctaaggc cagtgtcggc
     9541 ttgcagggaa tcgatattaa tgcccatcgc cggagcgctg agatcgacgg taatggtgtg
     9601 attactgcta ccggtgttgc caatggcatt gctcaccgag gcgttaatca cgtaggcgtt
     9661 gccgtccgcc agcgcggtga catcggcgct gccgagggtg tagctccagc taccgcccgt
     9721 ctgcaccgtg gtggtgtagg ttttgccgtt taaggtcagg gtcagcgtct gcccgacggg
     9781 cgcggtggtg gtgccgctga tcaccagcga tgagccgtgt tccgctgcat tcaccacatc
     9841 gtcgcccgca aaggtgttga tggtgacagt aggtacatcg ccgttcagcg tcacgccacg
     9901 gctggcgctg ccggggtttc cggcctgatc gctaaccgtc gcgctaatgg tgtagctgcc
     9961 gtcgcttaat ccggcaaact gctgcgccgg aataaacacc gaccagcttc cgccgctgcc
    10021 aaccgttgcg ctccaggttt gcccgttaaa ggtaacggtc accgtctggc ccacttcggc
    10081 ggtggtggtg ccgctaatgg tctgcccggc aagctgctcg gcgttgttga taatatcgtc
    10141 acccgccacc gtggcgatgg tgaccgttgg cgcaacggta tcgacggtca gggcgtgcgt
    10201 ggcctgtccg gggttaccgg cgcgatcgtt cacgctggcg gtaatggtct gcgcgccctg
    10261 cccgagcgcg gccagatcca ccgcaggcac gttcagcgcc caggtaccat tggcggcaac
    10321 cgtggcggta taggttttgc cgccaaaggt gacggtgacc gtctgcccca cttccgcgct
    10381 ggtcgagccg ttaagggtta acggctgctg ctgttcggcg gcgttgaggc ggtcgtccgt
    10441 cgaaacggtg ttaatgctga taaccggcgc ggtgagatcc accgccacgc tgcgcgaggc
    10501 cgtggcgctg tttccggcac tgtcctgagc ggaggcgcta accgtgtagc tggtgccatc
    10561 actgagtgcc gcaacatccg ccgcaggcac gttcacgctc cagctcccgt tgctctgaat
    10621 ggtggcgctg tagcctttgc cgttaagcaa cacggtcacc actgtacctg tcgcgaaatt
    10681 agtgccagtg ccgctcaggg tcagcgcact gcccgcttcg gcggcgttaa taaggttatc
    10741 gccgctgata gtgctgaccg agagcgacac tgccgccgtg ttcacctgta cgttgtgcgt
    10801 ctgcgtgctg ctgttgcctg cgctatcggt aatggtggcg ctgatggtca ccgtgccatc
    10861 cgcaaggccg gaaatcacgc tggcaggcac gccgacgctc cagttgccgc tggcatcggt
    10921 gctggtgaca tactgctgcc cggcgatggt caccaccagg cggtcgcccg ccaccgcgcc
    10981 cgtggcggtg ccgctgataa tctgcgcctg aatgtgttcg acgttgttaa tcacatcatc
    11041 gcccgccacg gtgttaaaac tgatcaccgg cgcagtggta tcgacggtca cgcctttgct
    11101 ggcgctgcca aggttacccg ccagatcgct gaccgtggcg gtcagggtgt agccgttatt
    11161 agcaagtgcg gttaagtcgc tggcgggcag cgttaaactc cagctgccgt ctgtctgcac
    11221 cgtggtctgg tagttagtgc cgttaagcgt gacggtgagc gtctgcccgg tttgcgcggt
    11281 gctggtgccg gacaccgtta cgcccgccgc cgcttcgctg gcgttgatga tgttatcgct
    11341 ggcaatggtg ttgatggtca ccgttggcgc ggcgctatcg acgctgtatt cgtgcgttgc
    11401 tgtggcgctg ttgccgttga cgttagtcac ccgcacctgg gcgctggcat cgccatcacg
    11461 cagggccgcc aaatccgccg ccggaacggt ggtgctccag ctaccgtttg ccgccaccgt
    11521 ggtggtgtag gttttgccgc caaaggtgac ggtgacggtt tgccccgctt cgacgccgga
    11581 cgtgctgccg gagagcgtta acgccgcgcc tttttcggcg gcgttaatcg catcatcgct
    11641 ggtgatgctg ttcatgctga tggcgacggc ggtgagatca acagtcagcg gatgggtaat
    11701 gctggtttgc gttccggcac tgttcgcccc gctaacggta atattcagcg ttcccgcagg
    11761 ccagttactg acatccgtcg caggaacgcc gacgctccag ctaccgttcg ccaacaccgt
    11821 ggcggcgtaa gtttggttgt taatcaccac cgtcaacgcc gtgcctgcgg caaagccgga
    11881 gctgctgccg gtgatcacct gcgcctgagc gtgttcgatg atattgacca catcatcacc
    11941 cgcgacggta tccacccgca ggccaggcag actggcatcg atggtgatat cgcgcgtgcc
    12001 gctaccggtg ttgccaacgg cgttggtcac cgaggcatta accgttaact caccgttacc
    12061 cagagcctgc cagtcggcgg caggtacgct cacattccag cttaaatcag cctggacggt
    12121 ggcggtgtaa gttttaccgc ccagttcaac ggtgacggtg ctgcccgccg ccgcaccggt
    12181 tacctggccg ctgatagttt gcgcgctgcc tgcctctgaa gcgttgataa tatcgtccgt
    12241 cgccaccgga ttaatggtga tgcccggcag cgcggtatta acctgcacgt tatggctggc
    12301 ggtgctgctg ttaccctgag cattggtgac gctcgccgtc acgctgtagg tcgcttcgcc
    12361 cagcgcactg accgccgagg caggcacggt aacactccag ttgccggagg catccgtggt
    12421 ggcgctgtag ttttgtccgt tcagggtgac ggtgatcgcc gtacccgcag gctgatcgct
    12481 ggtgccagaa agcgccagat cagatccttt ttccgtcgcg ttgatcacat catcgcccgc
    12541 gatggtgttg atgctaatca ctggcgcgct gaggctgacg gtcacggtgc ggctggcgtc
    12601 gtcgctgttg cctgcccgat cgctgacgct ggcggtgatg gtctgcgcac cgctacccag
    12661 cgccgtaaca tctgccgccg gaacgccaac gctccagttg ccggaggcat ccagggtggt
    12721 ggtgtaggtt ttgccgttga gcacaacgct caccacatcg cccgcttccc cgccgctact
    12781 ggtgccggag atcaccagcg cctgcgcatg ttcggcatcg ttgatgatgt catcgcccgc
    12841 cacggtgtta atggtgagaa ccggcgcggt ggtgtctacc gccagattat ggctggcgct
    12901 gccggggttg cccgctttgt cattgaccgt ggcgctgacg gtgtaattgc ttgcgttgag
    12961 cgcgcccagg gctgaagtgg gaacgctgac gctccagcta ccgtccgcct gcacagtgcc
    13021 ggtgtaggtt gcgccgttaa gggtgacggt caccgtctgc ccggtttcgg cggtgctggt
    13081 gccgctgatg gtgagcgggc ttccggcttc tgcggcgtta aggatatcgt cgctggcgat
    13141 ggtgttaatg gtgagcgtcg gcgcgctggc gtcaacgcta tacgcatggg tcgccgaggc
    13201 actgttaccg ctcgcgctgc taacgctggc ctgcacattc gccgcgccct ctggcagagt
    13261 tgccagatct gccgccggaa cgtttacact ccagctcccg ttggcggcga cgcttgcagt
    13321 gtaggtttta ccgccaaaag tgacggtgac ggtttgcccg ctctcaatcc cgctggtact
    13381 gccggaaaga gtcagattgg tgcctttttc tgcggcgtta atcacgtcgt cgctggcaac
    13441 ggtgttgatg ctgattgcca ccgccgttaa atcgacggtg aacggatggc tgacgctgac
    13501 tgggttattg gcgctgcttt gcccgtctac ctccacggtt aacggccccg caggccaggc
    13561 gctgacgttt gccgccggaa tgccaacgct ccagcttccg tccgcctgca ccgtggcgga
    13621 atacgccaca ctgttgatgg taaccgtcag cacagcacct gcattcaggc cgctactgcc
    13681 gccggtgatc accagcgcct gcccgtgctc gatgctgttg acgatatcat cgcccgccac
    13741 ggtatctacg cgcagccccg gcaggttggc atcaatggtg atatcccgcg tgccgctccc
    13801 ggtgttgcca ttagcgttgg tgaccgaggc ggtaatggtc aaatcaccat tgcccaacgc
    13861 ctggagatcg gctgtcggaa cgctgacgct ccagcttaag ttgctctgta ccgtagcggt
    13921 gtaagtgttc ccgcccagcg tcacggtgac cgtatcgcca gcggcggcgc gagtcaccac
    13981 cccgctgatg gtttgatcgg cccccgcttc ggcggcgttg ataatatcgt cgcctgccac
    14041 ggtgttgatg gtgacgtccg gcaggccgct gtcgaccagt aaattcgcct ggctgctggc
    14101 ggtgttgcct gctgcactgg tggcgctggc gctgaccgta tagttggcct gacctaatgc
    14161 cgccagatcg ctcaccggca cggttaaggt ccagttgccc gatgcgtcgg tggtggcgct
    14221 gtagttgtga ccgttgagcg tgacggtgac ctgcgcgccc gtcgccagcc ccgtactgcc
    14281 gccgctgatg gtcaacggct ggcctttttc atcggcgttc aggatgttat cgccgctgat
    14341 ggcgttaaag gtaatggtcg gcagcccggt attgaccgtc acctgatggg tagcgctgcc
    14401 gctgtttcct ccggcatcgg tgacgctggc attgatggtc accgtgccat tcgccagtgc
    14461 cgagacgacg cttgccggaa cgccgacgct ccagttgccg ctggcatcca gcaccgtggt
    14521 aaaggtattt gtgccgatag tcaccgtcac ggtgctaccg gttgccgctc cagtggcgga
    14581 gccgctgatg atctgcgcct gggcgtgttc cgtcgcgttg atcacatcat cgcctgccac
    14641 cgtgttgatg gtgacgaccg gaacggacgt atccaccgtc aggttgtgat taaccgaggc
    14701 cgggtttccg gctttgtcgc tcaccgccgc gttcacggtg tagttgctgg cggtcagggt
    14761 gcttaagtcg gctgacggta cgctgacgct ccagctgccg tccgtctgta cggtgccggt
    14821 gtagtttgtg ccattgagcg tgacggtcac cgtctgcccc gcttccgccg tgctgctgcc
    14881 ggtgatggtc agagccgctc cggcttcggc ggcgttaaga atatcatcgc ccgcaatggt
    14941 attaatggtc accgttggcg cgctggcatc aacgctgtaa gcgtgggtcg tggtggcgct
    15001 gttgccgtta acattgctga cgctggcctg tgcgctggca tcgccatcac gcagagccgc
    15061 catatctgcc gccggaaccg aggtgctcca ggaaccattc gcagccaccg tggcgctgta
    15121 agttttgccg ccaaaggtga cggtaaccgt ttgtccggct tcaacgccag aggtgctgcc
    15181 ggagagcgtt aacgccgcgc ctttttcggc agcgttgatc acatcatcgg cggtaatggc
    15241 gttgatgctc accgccaccg ccgagagatc gaccgtcacc ggatgcgtaa cgcttaccgg
    15301 atttccggca gaggtgctac cgctcgccgc aatcgtcacc gatcccgcag gccaggcgct
    15361 gacatccacc gccggaacgc cgacgctcca ggtgccatcc gccagcaccg ccgcaacata
    15421 ggtttgcccg ttaatggtca gcgtgacgtt gctgcccgcc gccagaccgg agctgctgcc
    15481 ggtaatcacc agcgcctgcc cgtgctcgat aatattaacc acatcatcgc ccgccacggt
    15541 gtcgacccgc agaccgggga gattcgcgtc tatggtgatt tcgcgcgtgc cgttaccagt
    15601 attgcctacg ctgttcgtca ccgaagccga aatggtcagt tcgccgttgc ccaactcctg
    15661 tagcgcggag gccggaacat cgacgctcca gcttaaattc gcctgcaccg tggcagtgta
    15721 agtcgccccg ccgagcgtga cggtcaccgt gtcgcctgcc gccgcacccg tcacctgccc
    15781 gctgatggtc tgttccacgc ccgcttcggc ggcgttaata atatcgtccg ttgccaccac
    15841 gttaatggtg acgcccggca gcgcggtatt aacctgtacg ttatggctgg cggaaccgct
    15901 gttgccatcg gcgtcagtgg cggctgcggt caccgtgtag gtggcttcac cgagggcgct
    15961 cacccgtgac gccggaacgg tcacgctcca gttaccggaa gcatcggcag tggtggtgta
    16021 attttgcccg ttgagcgtaa cggtaatctg cgtgcccgca ggctggttgc tggtgccggt
    16081 aatcgccaga tccgcgcctt tctccgcggc gttgatgatg tcatcgacgg caatggtgtt
    16141 aatggcgagc accggagcgc cgagcgccac ggtgacagtg tgcgaggccg tgccgctgtt
    16201 gcctgcggag tcggtgaccg tagcggtaat ggtcacatcg ccctgcgcca gcgcggaaat
    16261 cacgctggca ggcacgccga tgctccagtt gccgttggcg tccagcacgg tggtataggt
    16321 ggtcgtgcca atcgtcacgg aaaccgtatt accggtagtc gcgcccgttg ccgagccgct
    16381 gatgatctgc gcctgtccgt gttccgtggc gttaatgatg tcatcgcccg ccaccgtgtt
    16441 gatggtgacg accggagcgg caaggtccac cgtcagatta tgcgttgccg aagccgaatt
    16501 tctggctttg tcgctgaccg aggcgttaac ggtatacgag ctggcggtaa ggctagctaa
    16561 atcgccggtt ggtacgctga cgctccagct cccgtctgcc tgcacattgc cgctgtaatt
    16621 aacgccgttt agcgttacgg tcaccgtctg cccggcttcc gccgtgctgg tgccgctgat
    16681 ggtcagcgcc gatcccgctt cggcggcgtt aagaatatcg tccgtggcga tggtgttgat
    16741 ggtaacggag ggcgcagtgg catcaacgct gtaagcatgc gttgcctggg tgctgttccc
    16801 tgcgacattg ctgacgctgg cctggacgtt tgctgcgccg tctggcaagg ttgcaacatc
    16861 gacggcaggg atcgtcagcc cccaggtatt atccgcggca accgtggtgg tgtagctttt
    16921 gccgccgaag ataacggtga tggtttgccc cgcttccacg ccggaggtgg tgccggagag
    16981 ctgcaaatca gagcctttct cggcggcgtt aatcacatcg tcagtggaaa gggtgttgat
    17041 ggtgatcgcc acggcagcga gatcgaccgt gaccggatgg ctaatgctgg ttgtggttcc
    17101 ggcggtgttg gtgccagaga cggcaatatt gaccgtcccc gcaggccagt ttgtgacatc
    17161 tgccaccgga acaccaacgc tccagctacc atccgctaaa accgttgcgc cgtaggtgac
    17221 gctattgata accaccgtca acgccgcgcc cgccgccagt ccgctactgc taccggtgat
    17281 caccaacgcc tgagtgtgct cgatgctgtt aatcacatca tcgcctgcca cggtatcgac
    17341 gcgcagacct ggcaggttgg catctatcac gatatcgtgc gtcgcggtgc cggtattatt
    17401 tgcggaattg gtcaacgagg cgctgatggt cagttcaccg ttacccagcg cctgcaaaac
    17461 gttcgccggt acgctgacgc tccagcttaa atctgactgc acggtggcgt tatattgatt
    17521 gccgccaatg gtgacaatca ccgtattccc ggcaaccgcc gttcccgtga cctgaccgct
    17581 gatggtctgc gccacaacga tttcagcggc gttgataatg tcatcgccgg agacggtgtt
    17641 aatggtcagc gtcgggccgc taagatcaac ggtcacatcg tgagtcgtgc tgtcgctgtt
    17701 gcccgcgcga tcgctcaggc tggcggtgat ggtctggcta ccggtcgcca gcgccgtgac
    17761 atccgccgcc ggaatgccca cgctccagtt accggaggca tccagcgtgg tggtgtagtt
    17821 tttaccgttc agcgtgacgg ttaccacatc acccgccgcc gcgcccgtac tggtgccgct
    17881 gaccaccaac gcctgaccat gttcgatggc gttgataatg tcatcgcccg cgacggtgtt
    17941 aatggtgaga tccggcgcag tgatatctac caccagcgcg tggtcggcgc ttgccgggtt
    18001 acccgctttg tcgctcaccg tggcggtcac ggtgtaactg ctggcggtta atccgcttaa
    18061 atccgctgcc ggaacattca cgctccaggt gccatccgcc agaacggtgg tctggtacgt
    18121 gttgttattg agcgtgacgg tgagcgtctg cccggcctgc gccgtggtag tgccgctgat
    18181 ggtgatcccc gcgccagctt cgctcacgtt aaggatatcg tcgctggcga tggtgttgat
    18241 ggtcacaagc ggcgcggtgg catcaacgct atacgcgcga tcggcctggg cgctgttgcc
    18301 gttaatatta ctgacactcg cctgcacgtt gcccgcgccg tccggtagcg cagcgagatc
    18361 ggcaggcggc acattcaccg tccagctacc gttagcttcc actgtggtgg tgtaattttt
    18421 gccgccaaag gtgacggtca ccgtctgccc ggcttcgaca ccgctggtgc tgccgcttac
    18481 gaccagcgtt tcgccttttt cgacggcgtt gatcacatca tcaccagaga gagtgttaat
    18541 ggtgatggcg accccagcca gatcgacggt aaccggatgg gtaatggtgg aggttgttcc
    18601 ggcactgttg gtgccgctta ccgtgatatt caccgtaccc gctggccagt tgctgacgtc
    18661 taccgccgga acaccgaggc tccacgtgcc gtcggctaat accgtcgcgc cataagtaac
    18721 gttgttaatc tcgaccgtta acgccgttcc cgccgttaat ccgctgctgc tgccggtgat
    18781 caccagcgcc tgattgtgct caatgctgtt aataacatca tcgcccgcca cggtatcgac
    18841 ccgcaggcca ggcagattgg cgtcgatcgt gatatcgcgc gagccgctgc cggtgttgcc
    18901 gacgccattg gtgaccgagg cattaaccgt caggtcgccg ttgcccagcg cctgaatatc
    18961 cgccgccgga acgtccacac tccagcttaa attagactgc accgtggcgg tgtaggtgtt
    19021 gccgcccagc gtaacggtaa ccgtatcccc ctgcgctgcg cccgtcacct gcccgctgat
    19081 ggtttgcgca tttcccgatt cggcagcgtt aataatatcg tcagtcgcca ccgcgttaat
    19141 ggtaacggca ggcaaggcgc tgttgaccag cacattatga ctggcggaat tactgttgcc
    19201 tgccgtatca gtgacgtttg ccgttaccgt gtagttggct tcacccaatg cgctaaccgc
    19261 tgacgctggc accgtggcgc tccagttgcc gttgctatcg gtagtagcgg tgtaattttg
    19321 cccgttcagc gtcaccgtaa tggtggtgcc cgcaggctga ttactggtgc cggtgatttg
    19381 caggtccgcg cctttttccg tggctttaat cacgtcatcg gtggcgatgg tgttgatacc
    19441 aatggttggc gcggcgagat tcacggtgac cgtgcggctg gcgtcatcgc tgttgcctgc
    19501 cgcgtcggta attgccgcag tgatagtttg cgggccgctg ccaagcgcag tgacgtcagc
    19561 cgccggaacg ccgacgctcc agttgccgga agcgtccagc atggtggtgt aggttttact
    19621 gtttagtgtg acggtgatga catcacccgc ttcgccgcca gtgctggagc cggagatcac
    19681 cagcgcctgt ccgtgttcgg cggcgttaat aatgtcatcg ccggagacgg tgttgatggt
    19741 cagcaccggc acggtgagat ccaccgccag cccgtggtta gcggacgccg ggttacccgc
    19801 tttatcgctt accgaggcac taacggtgta ctggctggcg gtcagattgc tgagatccgc
    19861 cgtcggtaag ctgacgctcc agctgccgtc cgcctggacg gagccgctgt aagtcacacc
    19921 attaagcgtg acggttaccg tctgccccgc ttcggcggtg ctgctaccgc tgatggttaa
    19981 cggattgccc gcctcggcag cgttcagaat atcgtcggtg gcgatggtgt taatggcaag
    20041 cgtcggggcc gtggcatcga cgctgtaggc gtgggttgcc gaagccgtgt tgccgttaat
    20101 agtgctgacg ctggcctgca cggtggcgtc gccgtcgcgt aacacgctga gatcggcggc
    20161 gggtacggtg gttgtccagc taccatcgcc cgctacggta gcggtgtagg ttttcccgcc
    20221 aaaggtaacg gtgaccgttt gccccacttc cacgccggag gtgctgccgg aaagggttaa
    20281 atctgcccct ttttcggcgg cgttaatcac atcgtcgccg gaaacggtgt taatggaaat
    20341 cgccaccgcc gccagatcga cggtgaccgg atgggtgatg gtggatgttg ttccggctgt
    20401 gttagtgcca cttaccgtga tattcaccgt acccgcaggc cagttactca cgtctgccgc
    20461 cggaacacca acgctccatg tgccatcggc taatactgtt gcagcgtaag tgaccgtgtt
    20521 aatcaccacc gtcagcgccg cgcccgccgc cagcccgctg ctgctaccag ttatcaccag
    20581 cgcctgagcg tgctcgatgc tattgaccac atcatcgccc gccacggtgt caacgcgcag
    20641 acctggcagg ttggcgtcga tagtaatatc tcgcgaaccg ctgccagtat tgccaacgcc
    20701 gttggtcacc gaagcgttaa ccgtcagatt gccattgccg atggcctgaa tatccgccgc
    20761 cggaacgtcc acgctccagc ttaaattccc ctgcacggta gcggtgtaag ttttcccgcc
    20821 aagcgttacg gtaaccgtat cgcccgccgc cgcccccgtc acctggccgc tgatggtttg
    20881 cgcattgccc gattcggcgg cgttaataat atcgtcggtc gcaactgggt taatggtgac
    20941 gccaggcagc gcggtattga cctgcacgtt atggctggcg gaattgctgt tgcctgcgct
    21001 gtcggtgacg ttcgccgtca ccgtgtagct ggcttcgccc agcgcaccta ctgcggaagc
    21061 tggcaccgta gtgctccagt tgcctgcagc gtccgtcgtc gcggtgtaat tttgcccgtt
    21121 aagcgccagc gtaatcgtgg tgcctgctgg ctgattactg gtgccggaaa tttgcagatc
    21181 cgcgcctttt tcgctggcgt taattatgtc atcggtggcg atggtgttga tgtcaatggt
    21241 tggtgcggtg aggttaacgg tgaccgtgtg tgtctaattg tcgctgttgc ctgccgcatc
    21301 ggtaaccgtg gcggtgacag tttgcgggcc gctgccaagc gccgtgacat ccgccgccgg
    21361 aacgccgacg ctccagttgc cggaggcgtc cagggtggtg gtgtaggttt tactgtttag
    21421 cgtgacggtg acgacatccc ccgcttctcc gccggtgctg gaaccggaaa tcaccagcgc
    21481 ctgcccgtgt tcggtggcgt taataatgtc atcgcccgca atggtgttga tggtcagcac
    21541 cggaacggtg agatccaccg ccagattatg cgtggtgctc gccggattgc ccgctttgtc
    21601 attgaccgtg gcggtcaggg tgtggctgcc atcggtcaat gcctcaagat ctgccgccgg
    21661 aatattgatg ctccagctgc cgtccgcctg caccgttgcc tgataggtct gcacggtcgg
    21721 gctgttaagc gttatcgtaa caatctgccc cgcttcggcg gtggtactgc cgctcaccgt
    21781 cacgcccgca tcggcttcgc tggcgttgac gatattgtcg ctggcgacgg tattgataat
    21841 gatggttggc gcgctgctgt cgacgctgta gttgtgcacc gccgaggcgc tattgccgtt
    21901 gatattgctg acgctggcca gtgcggaggc gctgccctcg ggtaatgacg ccagatcggc
    21961 ggcgggtacg gtggcagtcc agctaccatc gctcgctacg ctggcagtgt aatttttccc
    22021 gccaaaggtg acggtgacgg tttgaccggg ttctacgtta gtggtggtgc cggaaagggt
    22081 taaatcagcg cctttttctg cggcgttaat cacatcgtcc gtggcgatgg tgttgatggt
    22141 gatcgctgcc ggagtgagat ccaccgtcac cggatgcgta atgctcaccg agtttccggc
    22201 gctactttcc cctgaaacgg caatattaac cgtccccgca ggccaggcgc taacctgcgc
    22261 cgccgtgacg cccacgctcc agctaccatc ggcctgcacc gcagtggtgt attccacatt
    22321 attaatcgta acggtaagcg gcgtgctttc agccagcccc gagctgctgc cggtgaccac
    22381 cagcgcctgc ccgtgctcga tgatattgac cacatcatcg cccgccaccg tatcgacccg
    22441 caggccgggc agattggcgt cgatggtgat atcccgcgtg ccgctgccgg tgttgccgtt
    22501 ttgattggtg actgaggcat taaccgttaa atcgccattt cccagcgcct gaatatctgc
    22561 cgctggaacg tccacgctcc aggtgagatt gctgcccacc gtcgccgtat aagtattacc
    22621 acccaacgta atagttaccg tgtcgccatc ttccgcgcca gtcacctgcc cgctgatggt
    22681 ttgcgccacg cccgcttcgg cggcgttaat aatatcgtcg gttgccaccg gattaatggt
    22741 cacaccgggc agcgcgctgt cgaccagcac gttatggctg gcagtggcgc tgttgccgat
    22801 atcgctggtc accgccgccg ttaccgtata gttggcctgg cctaatgctg taaccgccga
    22861 tgccggaacg gtgacgctcc agttgccgga ggcgtcggtc gtggtggtgt aattttgccc
    22921 gttcagcgtc acggtgatgg cggtgttaac aggctgatcg ctggtgccgg taatctggag
    22981 atcggcccct ttttcgctgg cgttaatcac atcatcgccc gcaatgctgt taatgccgat
    23041 aaggggcgcg gcggtattga ccgtgacggt caatgactgg ctgcccgtgt ttccggcttt
    23101 gtcggttacc gagacgctga caggataact gccgtccgcc agcccactga ccaccgaggc
    23161 cggaacgccc agactccagt tgccggaacc atccaccacc gtggtgtaat ccacattatt
    23221 cagcgtcacg gtgacgatat cgcccgcctg agcgccagta accgtgccgc tgatgatttg
    23281 cgcctgagca tgttccggcg tgttgatgac gttatcggtc gccaccggat taatggttac
    23341 caccggcggc gtggcatcca ccagcgccac gcgtgaaacg ctgctcggat taccggcgac
    23401 atcgttcacg ctggcggtga ccgtagcgtt gccgtcagcc agtgcgccca cgttggcggc
    23461 gggcacagtc acgctccagg tgccgtctgg ctgcacgacg ccctgatacg tctgcccatt
    23521 taacgtaacg gtcaccgtct gccccggctg agcgtcggtg gtgccggtga tgggcagatc
    23581 ctgcccgctt tcggcggcgt taatgatgtt atcagcggtc agtggatcaa tgcttaaggc
    23641 cggggcctgg ctatcgacgg taatggtgcg ggaagtgttg ccggtattgc cgctgagatc
    23701 cgtcacagaa gcggtaatcg ccaccgcgcc gtcaggcaga tttcccatcg cggcggcagg
    23761 taccgtcaga ctccaggagc catcggcctg cacggtagtg gtaaaactct gtccagccag
    23821 ggttactgtg acggtttgtc ctgtctcagc ctgttgagtg ctaccggtaa gggtcagtgg
    23881 cgcacctttt tctgcgccac tgataatgtc atcgccggag acggtgttaa tggtgatcaa
    23941 cggagaaaca ccgaccagcg tgattgctcg agacgccgcg ccagtatttc ctgcagcatc
    24001 tgtcacggtg acgaaaatcg tattggctcc gcgatccagc gtgcgggtta ccgctgggtc
    24061 cagcgccaca ttccagctgc catctgccag cacgatgccc gtcaggacat gagtgcccaa
    24121 tttcaccgtt accgtatcgc ctggagaagc accgctgact tgtccggaga tgatctgcgc
    24181 gacggcctgc tcggcattat taagaatatc gtcgcccgcc acggtattaa cactgaccac
    24241 gggtgctgag gtgtctaccg tgaaattcgc gctgtggctg gtggtgttcc ctgcccgatc
    24301 gctgacactg gcattgacag cgtaattacc ctccgccagt gcctggactt ccgtggcagg
    24361 cagcgtcact tgccaggttc cgtcgctacc gacggttgct gtatggcttt tcccgttgac
    24421 ggtcagtgtt acggtttgcc ccgcttctgc attcgacgtg ccgctcagta ccagcgcgac
    24481 gttatgttcc gcagcactga taatgttgtc ctgagcgatg gtgttgatgg caagcgttgg
    24541 cggttgggta tcgaccgtta gtagctgcgc gccggtgacc gtattgcctg tggcgtcttt
    24601 cccactgacc agcaccgcct gattcccttc gcctaacgcc tgtgcatcgg cggtcggcac
    24661 ctgcacgctc caggcaccgt tagcgcctac gctggtcgtg taggttttgc cgtttagcgt
    24721 aacggtgagt tctgttccct gcgccagatg gctactggtg ccagaaagcg taaacccggc
    24781 ggcctgttcg ctggcgttaa taatgttatc gcctgcggtg gtggcaacct gcacgctggc
    24841 ctgactattg tcgatggtta acgttatcga accggcgtca gtgatggtac tgccgacaat
    24901 ttgcaccact gaccaggtat gttcacctgc actttgcgtg ggtaacgaga cactccagtg
    24961 accgttgttg tctacctgag tactggcaat agtattgccg ttactgtctt taatctgaat
    25021 agtcgccccc gcctgcccgc tgccgctaaa ggttggcgtg gtgtcatcgg tgatgtcatt
    25081 ggtggctaga atgccgcgct gatcgccctg attatcggta actaaaaatg atggtgtggc
    25141 gttgccaggc tcagcaggtg gcgtagggtt attaatcact tctgttttcg agtcgccatc
    25201 gccaccgctt gccagcagtg caccaagcgc gccaccacct accgccgccc ccgccagcca
    25261 accccacggg aaggcgctgg tctgagcaac ggtatcaaga aatggcgcaa tgctttcaat
    25321 cgcagtggtc tgggcagtca gttctacggg ggctaatcca cccgcagcag tatcggcaaa
    25381 ggtgatatgg gttagctgtt gaccatcggc aaacaccagt tccgattgtt cagctgtatt
    25441 cgccgcttgc aggaaataac cgttgcagcg gatcaccgtg ccgtcctgca tatagatcag
    25501 caggtcattt ccttcacgga cgtaacgcgc aacggcagag gcctgagcat gaacctgcac
    25561 cacggaaggc tcataaataa caaccgtgac gtcgccagga ttaatcagcg tttgtttgac
    25621 ggatgtttta cgcgaaataa catcaataat caggctcata atggcattcc tgataaatgg
    25681 cgcacatcct tagcgagtcc atttatctaa ataagacaaa ggtaatcact taacaacggc
    25741 ccactattaa taatggcagg attatttcaa gtcgggtaag tcgttgtata agccctgttt
    25801 aattcccgct aatggaataa ggttattcac cgcagcggca taatttacgg cggcgaccca
    25861 gccatcataa ttggcattta tttcagccga ttgcgcctga aagacatctt gttcgacggt
    25921 taatagatca ttcagactgc gtttacccaa tttatattca ttttggtaca catctcgcgt
    25981 tttgtgcgca ctttcggatt gcgctaaacc ggcttcttca cgaccccgtg cgccggtcca
    26041 gttcgcatat gccacagacg ctcgttgcag cacatccagt tttgcctgtt cgacctgcga
    26101 ggcagagatt ttttgttgcc cttcggcctg ttgcacctgg gcagaaaccg cgccgccctg
    26161 atacagcggc gcgttaacgt tcagttgtag ctgatcatcc caatacgagc ggtcgctggt
    26221 ctggtagcgc gttttacccc cctgaatact gagcgttggc cagtattgcg ctttcgtttt
    26281 ttccacgccg tactgtgctg actggcgtaa gttttctgcc gccagcacca gcgggatgga
    26341 ctggtaatca atattcttca gcgataccgg ctgctcggct aattcagcag gtggcgcggc
    26401 tatcgcctcc ggctgtacgc cagtgagcac cgccagttgc gctttggcgc tcgccatctg
    26461 cgcctgatat tgctccagcg ttgagcgcat tccggcaata cgcgtctggg cctgtaattc
    26521 atccgacgat gagttcaggc ctgcgttagc gcgcaatgcc gccatgttgt agacgttttc
    26581 cagcgagtga atattgcgtt gagccgcatc gcacaaagcc tgataacgac tgacttccat
    26641 gtaggcagtc gccgttttct ctgcaacatc ggttaaggtt gccatcaatt taaagcggta
    26701 gctgtcacgg gcggcagttt gcagattgat atcgttgtta gttttaccaa agtcatacac
    26761 cagttgtgtc agggttatgc catacgaaac gttgttatca agcctgccgc tggagtccgt
    26821 ttgtcgggac ggccccgcgt tgccggttaa tccaacgtgc ggataccagg cacttttggc
    26881 ttcatcaatt tgcgcttcgc caatacctat ctgcgccgcc tgttgagtga caccgggatt
    26941 tcgcgcaaag gcgcgtaata tactttcctg taaagataat tgggcaactg gcgttaccgc
    27001 tggggcattc gcccattgtg gcagcggtgc agcaattgca gcttctccca tcaccataaa
    27061 acttataaaa aaaataagtc cattattttt acggttttta aactggaatc gcgccataga
    27121 taagtcactt ccttccaggt cattatttta actggttttt ataaccagtc tcgttttatt
    27181 aagaagggta ataacttgtg catctctttt gtcgatcatt gatatgggca aagaaatgca
    27241 gctaaaaagc ttatacgtta tttatttaga
//
LOCUS       DAZKFZ010000049        27257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000049 DAZKFZ010000000
VERSION     DAZKFZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..188
                     /locus_tag="ACHOQW_001856"
     CDS             <1..188
                     /locus_tag="ACHOQW_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9572941.1"
                     /translation="PNDKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEPA
                     SKVTHWMPLPEPPQEVK"
     gene            276..518
                     /locus_tag="ACHOQW_001857"
     CDS             276..518
                     /locus_tag="ACHOQW_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9572942.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            522..656
                     /locus_tag="ACHOQW_001858"
     CDS             522..656
                     /locus_tag="ACHOQW_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9572943.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            675..929
                     /gene="xisR"
                     /locus_tag="ACHOQW_001859"
     CDS             675..929
                     /gene="xisR"
                     /locus_tag="ACHOQW_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9572944.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            963..2249
                     /locus_tag="ACHOQW_001860"
     CDS             963..2249
                     /locus_tag="ACHOQW_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9572945.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            2270..2971
                     /gene="mlrA"
                     /locus_tag="ACHOQW_001861"
     CDS             2270..2971
                     /gene="mlrA"
                     /locus_tag="ACHOQW_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP9572946.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            3031..3138
                     /gene="yohO"
                     /locus_tag="ACHOQW_001862"
     CDS             3031..3138
                     /gene="yohO"
                     /locus_tag="ACHOQW_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP9572947.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(3119..3850)
                     /gene="yehW"
                     /locus_tag="ACHOQW_001863"
     CDS             complement(3119..3850)
                     /gene="yehW"
                     /locus_tag="ACHOQW_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP9572948.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(3855..4781)
                     /gene="yehX"
                     /locus_tag="ACHOQW_001864"
     CDS             complement(3855..4781)
                     /gene="yehX"
                     /locus_tag="ACHOQW_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP9572949.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(4774..5931)
                     /gene="yehY"
                     /locus_tag="ACHOQW_001865"
     CDS             complement(4774..5931)
                     /gene="yehY"
                     /locus_tag="ACHOQW_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP9572950.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(5938..6855)
                     /gene="osmF"
                     /locus_tag="ACHOQW_001866"
     CDS             complement(5938..6855)
                     /gene="osmF"
                     /locus_tag="ACHOQW_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP9572951.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGSDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7107..9404)
                     /gene="bglX"
                     /locus_tag="ACHOQW_001867"
     CDS             complement(7107..9404)
                     /gene="bglX"
                     /locus_tag="ACHOQW_001867"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP9572952.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9600..11315
                     /gene="dld"
                     /locus_tag="ACHOQW_001868"
     CDS             9600..11315
                     /gene="dld"
                     /locus_tag="ACHOQW_001868"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9572953.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(11353..12285)
                     /gene="pbpG"
                     /locus_tag="ACHOQW_001869"
     CDS             complement(11353..12285)
                     /gene="pbpG"
                     /locus_tag="ACHOQW_001869"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP9572954.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(12459..13046)
                     /gene="yohC"
                     /locus_tag="ACHOQW_001870"
     CDS             complement(12459..13046)
                     /gene="yohC"
                     /locus_tag="ACHOQW_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP9572955.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            13216..13794
                     /gene="yohD"
                     /locus_tag="ACHOQW_001871"
     CDS             13216..13794
                     /gene="yohD"
                     /locus_tag="ACHOQW_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9572956.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(13924..14685)
                     /gene="yohF"
                     /locus_tag="ACHOQW_001872"
     CDS             complement(13924..14685)
                     /gene="yohF"
                     /locus_tag="ACHOQW_001872"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9572957.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(14738..16174)
                     /gene="mdtQ"
                     /locus_tag="ACHOQW_001873"
     CDS             complement(14738..16174)
                     /gene="mdtQ"
                     /locus_tag="ACHOQW_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP9572958.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            16398..16481
                     /gene="yohP"
                     /locus_tag="ACHOQW_001874"
     CDS             16398..16481
                     /gene="yohP"
                     /locus_tag="ACHOQW_001874"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP9572959.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(16869..17819)
                     /gene="dusC"
                     /locus_tag="ACHOQW_001875"
     CDS             complement(16869..17819)
                     /gene="dusC"
                     /locus_tag="ACHOQW_001875"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP9572960.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(17979..19172)
                     /locus_tag="ACHOQW_001876"
     CDS             complement(17979..19172)
                     /locus_tag="ACHOQW_001876"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFP9572961.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(19187..19831)
                     /gene="maiA"
                     /locus_tag="ACHOQW_001877"
     CDS             complement(19187..19831)
                     /gene="maiA"
                     /locus_tag="ACHOQW_001877"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFP9572962.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(19840..20541)
                     /locus_tag="ACHOQW_001878"
     CDS             complement(19840..20541)
                     /locus_tag="ACHOQW_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9572963.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(20556..21584)
                     /gene="gtdA"
                     /locus_tag="ACHOQW_001879"
     CDS             complement(20556..21584)
                     /gene="gtdA"
                     /locus_tag="ACHOQW_001879"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFP9572964.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(21596..22954)
                     /locus_tag="ACHOQW_001880"
     CDS             complement(21596..22954)
                     /locus_tag="ACHOQW_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9572965.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            23081..23989
                     /locus_tag="ACHOQW_001881"
     CDS             23081..23989
                     /locus_tag="ACHOQW_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9572966.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            24120..24518
                     /gene="yohJ"
                     /locus_tag="ACHOQW_001882"
     CDS             24120..24518
                     /gene="yohJ"
                     /locus_tag="ACHOQW_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9572967.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            24515..25210
                     /gene="yohK"
                     /locus_tag="ACHOQW_001883"
     CDS             24515..25210
                     /gene="yohK"
                     /locus_tag="ACHOQW_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP9572968.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            25340..26224
                     /gene="cdd"
                     /locus_tag="ACHOQW_001884"
     CDS             25340..26224
                     /gene="cdd"
                     /locus_tag="ACHOQW_001884"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9572969.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            26374..27093
                     /gene="sanA"
                     /locus_tag="ACHOQW_001885"
     CDS             26374..27093
                     /gene="sanA"
                     /locus_tag="ACHOQW_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP9572970.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            27096..>27257
                     /locus_tag="ACHOQW_001886"
     CDS             27096..>27257
                     /locus_tag="ACHOQW_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP9572971.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLF"
BASE COUNT         6538 a         7419 c         6943 g         6357 t
ORIGIN      
        1 tgccgaacga taaacagtat gtttggtgtt gggggaagtc ttacggctgg actgagtgcg
       61 ataccttcga agggtattac gattgttcga gaaacaaatg gtgggcagtt actgacaatg
      121 gggaagaacc ggcatcgaaa gtaacccact ggatgccgct accggagccg ccgcaggagg
      181 tgaaatgatg aattggcctg aagcattcac cgcagttgga gttgcaatag cggtggcatt
      241 tattctgtat tcgcttttcc gctggggata aaggaatgtt cgctctgatt caacgtggtc
      301 agatatacac ggacagagcc ggataccctg tggtgattac tcgcagtact cagcactcag
      361 tgttctttcg acgcatggac gggcgctccg gacgggtacg cattggtgag ttcaacaacc
      421 tgttcgaaca tattgaccaa caggagtacc gcaaaattct ggcgggcact gagcaggaaa
      481 tgcgcctgaa aaaattacgc gcaatgcaac ggaggtgata catgcatacg gcttttgagt
      541 tctgggttcg caagacattc ggcaatcgct acgacctgac ccgtgatgtc gacggcttct
      601 actgccgtga agttgtgaaa cgaatgtttg acgtgtggtg ccactgccgt ggatgaaagt
      661 tttatgaggt tggcatgcag acaatcatct atcagataac ccccagcaaa tggtgtacgg
      721 agagagtcct tattgcatca acagggctaa agcccggcac catcgagcgg gccagaagaa
      781 agtcatggat gcagggaaaa gaataccgcc attacgctgt agaaggtgat cctgggcatt
      841 acagtgaatg cctgtacaac atcgaagaaa ttatgcgatg gatcgaaaac cagaaacaac
      901 caggtgccaa aaatgcaagt tccggttaac ctgttaatgc tcctggacgt ctgggaggtt
      961 taatgagtaa cgcatcatac ccgacaggcg ttgaaaacca tggcggatca ctccgtatat
     1021 ggtttcacta taatggcaaa cgtgtcagag aaaacctcgg tgttcctgac acagccaaaa
     1081 accggaagat cgctggtgaa cttcgcactt ccgtttgttt tgcaatcaga atggggagtt
     1141 tcgactacgc cgcgcagttc cctaattccc ctaacctgaa acactttggt ctgggaaaaa
     1201 gagagataac cgttaaggca ctttcggaaa aatggttgga ccttaagaaa attgagattt
     1261 gtgcgaatgc acttaaccgt taccagtcag taattaaaaa catgttacca atgttaggtg
     1321 aaaaaaaact ggtttcatcc ataacaaaag aggatttact tttcgtaagg agagatttgt
     1381 tgaccggtta ccaaaagctt tctaatggaa agacttcttc cataaaaggg cgctcagtgg
     1441 tcacggtaaa ctactatatg acaaccatag ctggaatgtt tcaatttgca acagataatg
     1501 gttatacctc aggaaaccca tttaacggtc tggctccctt aaaaaagtcc aaggtaaaac
     1561 cagatcctct cacccgtgac gaatttattc gttttattga ggcttgccgt catcaacaaa
     1621 caaaaaacct gtggattctc gctgtataca cgggtattcg tcacggggag ttggtatcgc
     1681 tggcatggga agatatagac cttaaagcaa ggactataac catccgtaga aattatacaa
     1741 aacttggcga attcactcca ccaaaaaccg atgcaggcac cggaaggaca attcatctgg
     1801 ttcaaccagc tattgatgct cttaaaagcc aggcggaaat gaccatgctt ggaaagcaac
     1861 attctgtaga ggtgaagcag agggaatatg ggagaactgc tgtgcataaa tgcacttttg
     1921 tttttagtcc tcaggtaaca aaacagcagc agttgtccgg acctcactac aaggttgact
     1981 ccatcaggga gtcatggaca agtatcttaa aacgcgcagg tctgagacac agaaaatcgt
     2041 accaatccag gcatacttat gcatgctggt cacttgccgc aggagctaat cctagtttta
     2101 tcgcaagcca gatgggccac acaaacgcac aaatggtatt caatgtttac ggagcatgga
     2161 tgaaagacaa caatcacgaa cagatagaac tccttaacaa aagactatct gaaagtgtcc
     2221 catgtatgcc ccataagaaa gcagggtaaa ataaaaactt gcaaaatcaa ttagtttacc
     2281 cttaatccct gtcacgttac gcgcgtggca gaggcgttac ggattgctga aaccgcaacg
     2341 gacagacggc ggtcatcggc tgttcaacga tgccgatatt gaccggatcc gcgagatcaa
     2401 acgctggatc gacaacggcg tgcaggtcag caaagttaaa atgctgctca gtaatgaaaa
     2461 tgttgatgtg cagaacggct ggcgcgatca gcaagaaaca ttactgacct acctgcaaag
     2521 cggcaatctg catagcctgc gaacgtggat caaagagcgc ggtcaggatt accccgccca
     2581 gacgctcacc acacatctgt ttattcctct gcgccgacgg cttcagtacc aacaaccgac
     2641 tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatcg ccatttgtct
     2701 ggcttcggca cgtaaaaaac agggtaaaga tgcgctggtg gttggctgga atattcagga
     2761 taccacccgt ctgtggctgg agggctggat tgccagtcaa caaggatggc gcattgatgt
     2821 cctcgcccac tcgctcaatc aactacgccc tgaactattc gaaggccgta cattgctggt
     2881 gtggtgcggt gaaaatcgaa cctccgccca acagcagcaa ctcaccagtt ggcaagaaca
     2941 aggccatgat attttcccac tcggcattta atgattcgtt aacaaatgcg ctttactgta
     3001 caatcctttc gttaacataa ggagtgcatt atgcgcatag ctaaaattgg ggtcatcgcc
     3061 ctgttcctgt ttatggcgtt aggcggaatt ggtggcgtca tgctcgcagg ttataccttt
     3121 attttgcgtg ctggctaagc gcctgcacca gcctttcaaa caggcggtct gcgatgatcg
     3181 ccgccagtgc caccagtaac gccccctgga tcacatacgc ggtattaaat ccgctaagcc
     3241 cgatgatgat gggcgtaccc agcgtgctgg cccctaccgt tgaggcgatc gtcgccgtac
     3301 caatgttgat aatcaccgag gttcgcacgc ccgccagaat caccggagcc gccagcggta
     3361 gctcgacctt acgcagtcgc tgacctcgac tcattcccat acctttcgca acttctgtca
     3421 cgctggcatc aatcgctccc agcccggcaa gtgtcgcctg caggacgggc agcacaccat
     3481 aaaggatcaa ggcgataatc gctggttgca gaccaaagcc tatcaccgga acagcaatcg
     3541 ccagcactgc gacaggcgga aaagtctgcc caacggcggc aatagtttcc accagtgggc
     3601 gaaattccgc gccccacggg cgagtgactg caattccggc accagtgcca atgatcaccg
     3661 caaacaaact cgaaattccc accagccaga aatgagccag tgccaaagct gcaaaacttt
     3721 cttgctgata aacgggtcgt ggcagttgtg ggaacaaggc agcaaacagc ggctggctgt
     3781 aaggcagcca gaaaatcagt gccacaaaca aagcaatgag ccagaacagc ggatcgcgca
     3841 acatcttcat acgcttacgc ctccaccagc agatcctgaa aatgcagcgt gccgctaggc
     3901 tggccctgcg tgttcaccac cggcagcacc tcgcatcccc gcgcgacaaa cagggagagc
     3961 gcatcgcgca gcgtcatctc ttctgccagt gcctcacctt ctgcccgttc ttcgcgacgc
     4021 acgtaatccg ccacactacg taacgaaagc aggcgcacac ccagttcact acgtccaaaa
     4081 aactggcgga caaaatcatt cgccggacga gtcagcatcg tcagcggatt cccctgctgc
     4141 accacttcac cgtgatccat caataccaga tgttctgcca gccgtagcgc ctcatcaata
     4201 tcatgcgtga ccagcacaat ggtacgcccc agcaaacggt gaatgcgcgt catctcttgt
     4261 tgcaacgcgc cgcgcgttac cgggtccagt gcgccaaaag gttcatccat cagtaagact
     4321 tgcggatcgg cagccagtgc gcgcgctact cccacacgtt gctgctgacc accggaaagc
     4381 tgatgtggat aacgctcacg taaatttgac tccagcccca gtagcgccat taattcgtcg
     4441 atacgatcgt caatcctcgc ccgcgaccat ttttgtaatt gcggcacggt ggcaatgttt
     4501 tgtgccacgc tccagtgggg aaacaggcca atagattgaa tggcatagcc catacggcgg
     4561 cgcaactcca gtactggcag cgagcgaatt tcttctccag caaagcggag ctctccgctg
     4621 tcatgttcca ccaggcggtt aatcattttc agggtggtgg atttgccgga gccagatgtg
     4681 ccaatcagca ccgaaaaact cccttcctga aaattgagat tgagatcgtt aacggctttt
     4741 tgtgcgccga acagtttgct gacatggcta aattcaatca ttacgtttca ccttcaacag
     4801 tgcgattagt aaatcgaaca gcgcatcgat cagcaccgcc agaacaatta ccgggatcac
     4861 ccccagcaac actaaatcaa tggcgctgct tagcagcccc tggaaaacca gcgcaccaaa
     4921 accgcctgcg ccgattaacg ccgcaatcac cgccatgcct acagtttgca ccatcaccac
     4981 ccgcaggctg cgcagaaata ccggtaacgc cagcggtaac tgaacatgca ggaatcgctg
     5041 cgccccgctc atgcccatcg ctctggcgct ctccagcaca tcgcgcggga tctggttcaa
     5101 gccgactacc acgccgcgca ccagcggtag caaggcatag agcaccagcg caatcagtgc
     5161 gggtgtcatt ccggttcctg ctatgccaag cttccccagc cacggaaagg ccgtaaccag
     5221 tccggcaagc ggcgcaatca gcaggccaaa gagcgccacg gaaggcacgg tctgaatgac
     5281 attgagcaga gaaaaaatcg ccccctgccg cgcggtggaa aagtagcacc agatgcccaa
     5341 cggcacacca atcactaacg caggcagcac cgcaccaaac agcaacgtca gatgttgtgc
     5401 cagcgcgtcg tcaaacacat cctgacggtt ggcgtattct ttcattagtg agagatcgtt
     5461 aagcgtgccg gagtacagca accacagcgg aataatggca atctgcatat gcaacaacca
     5521 gcgccacagc ggatgcgtgg agattcggcg gatggcatcg ctacaggcca gcaatgccag
     5581 cgccgaagcc aaccagaaac cactgccgag gctggtacgc gccagcgcac tgccattttg
     5641 cgccagttgg gtcgccgcct ttccagctcc ccacaccagc aatacgaaga cgaattgcgc
     5701 cagaatgagt gcaaaaatgc tgcctttttt agcgggaata aaacaggctg tcagccaggc
     5761 gcaaccaacg cccaccagca tccagatcgt ttgcggccac agttgccaga gatgacgccc
     5821 ttcacccgag actaaacgat taggcgcgta gctgataaac ggcagcgctg ctgcgattgc
     5881 cgtcagcaac agcagcagcg ccagaacagg attaatacgt agataagtca cgggaaatta
     5941 cttcgtccac cctttttgtt tcaagtagtc ggcagccact tttttggcat ccagtccttc
     6001 cactgcaatg ctggcattca gttgctgcaa tgtttttgca tcgaggctgg cgaagactgg
     6061 ctgtagccac tgtgccattt gcggatactc tttcagcacc gactcacgca ccactggtgc
     6121 aggcgcgtag ataggttgca cgccttgcgg atcgcttaag gtttgcagcc cctgcgccgc
     6181 gaccggaccg tcagagccgt aagccattgc ggcattaacg ccagaagttt gctgggcagc
     6241 ggctttaatc gttaccgccg tgtcgccgcc agccagtgac agtaactgat cctgatcgag
     6301 tttaaagtcg taggcttttt caaacgcggg taacgcatcg gcgcgttcga taaactcagc
     6361 cgaggctgcc agtttgaagg tgccgccctc tttcagataa cgcctcaggt cagcaagcga
     6421 agtgagtttg tttttctctg ccacatcctg acgcacggcg atggtccagg tgttatttgc
     6481 aggtgcgggc gtcagccaga ttaacttgtt ttgctctgca tcgagttttt tgactttctc
     6541 gtaaccttgc tgcgcgtttt tccacgctgc atcgttttca tctttaaaga aaaacgcgcc
     6601 attgccggta tattccggat agatatccag ttcaccggaa gtaatcgccc cgcgcaccac
     6661 aggagtcgtt ccaagttgca ctttattgac cgttggtact ccgtggcttt cgagtacttg
     6721 caaaatgata ttgccgagca gcgcgccttc ggtatcgatt tttgaaccga ctttaacggg
     6781 ggaagccgcg tgcagcggca ggctcacggc tgccaacaaa accagtgaac ctgcccagac
     6841 ctttgagagt ggcatgatgc tttcctcatt cttttactgt tgttttcagc gaattaagag
     6901 aaaagcatag ttgataatgg cgggattagc ctgagcggac ggattcagtt gcagaatcag
     6961 ataaatactt aagaggcata ttcggtccgg cgtttcctgc cggatgcggc gcgagcgcct
     7021 taaccggcct acaaagggcg aaaattcagc aagttaagat atgaacatca ggcaaagggc
     7081 gcaaacgtcg cgcccttgct aaagcattac agcaactcaa actcgccttt cttaacgcgt
     7141 gcggaatcag tgccgataaa gacattgaac ttgccaggct cggcgtcata tttcatctgt
     7201 tgattccaga acttcagcgc ctcgatatca atcgggaagc tgacggtctg ggtttcgccc
     7261 ggcttcaggg tgattttctc aaagcctttc agctgcttca ctggacggct catggaagcc
     7321 gtcacatcct gcaagtacat ctgcactacc gtggcccctt cgcgtttacc ggtgttcgtc
     7381 acctgcacgc tggccgtcac tttgccgtca cgcttcatgg tcggcgcaga aagtttcaca
     7441 tcagagacgg tgaaagtggt atagctcaga ccatagccga acggataaag cgccccgtta
     7501 gcttcatcaa aataacgcga agtgtatttg ttcggcttgt cggcattata cggacgaccg
     7561 gtgttcagat ggctgtagta caccgggatc tgcccgacag aacgcgggaa ggacatcggc
     7621 agcttaccgg acgggttgta atcgccaaac aacacatcgg caattgcatt accgccttca
     7681 gtcccggcaa accaggtttc cagaatcgca tcagcctgct gatcttcttt caccagcgcc
     7741 agcggacgcc cgttcatcag caccagcacc agcggtttgc cggtggcttt cagggcggca
     7801 atcaaatcac gctggctttg cagaatagta atatcggtac ggctggaggc ctcgtgcgcc
     7861 atcccctgtg cttcaccgac tacagccacc accacatcag attgtttcgc cgtctgcacc
     7921 gcttcatcaa tcatctcttg cggcgaacgc ggatcgactt tgaccgcttc ttcatactga
     7981 ttcaggaaat cgataatgcc tttgtcactg gtaacgttcg cccctttggc atacagcact
     8041 ttaccgtttt caccgacggc atttttaatc ccggtcagca cggtcacgga ttgatcggca
     8101 acacctgccg ccgaccagct gcccatcacg tcacgtttac tgtccgccag tggcccaacc
     8161 accgcaatgg tggctgattt tttcagcggt aacgtttcga gacggttttt cagcaacacc
     8221 aggctttcac gcgccacttc acgcgcttct ttgcggtgca ggcggctttc ggcattggta
     8281 tccaccgggt cagactcttt cggaccgaga tggctgtatg ggtcgttaaa caaccccata
     8341 tcatatttaa cgttcagtac atggcgggca gcatcgtcca gctctgccat cgtcacttta
     8401 ccggatttga tcaacccagg cagatacttc gagtagtact cgtcgctcat gctcatgttg
     8461 attccggatt tcagcgccac gcgcactgca tcttccggat ctgccgccgt gccatgttta
     8521 atcagctctt tgattgcacc gtgatcggaa acggtgatgc ctttaaagcc ccactggtcg
     8581 cgcagaacat ctttcagcag ccaggagtcg gaggtggctg gcgtgccgtt cagcgagttc
     8641 agcgccacca tcaccgcgcc gctgcctgcg tccagccccg ctttgtacgg cggcatataa
     8701 tcattaaaca ggcgctgcgg actcatatcg acggtgttgt actctttacc gccttctacc
     8761 gcgccgtatg cggcaaagtg tttgacgctg gtcatcaccg agtaacgatc tgccgggctt
     8821 ttaccctgca tcgcttccac catggtttta cccattattg aggtgagata cgtatcttcg
     8881 ccaaaacctt cggaagcacg tccccagcgc ggatcgcgcg agacatcgac catcggtgcc
     8941 caggtcatat tcaggccatc atctgccgct tcataagcag agacacgtcc gaccgttttt
     9001 actgcatcga ggttaaaaga cgaggccaga ccgaggctaa tcgggaacac cgtgcgctga
     9061 ccgtgcagca cgtcgtaagc aaagaaaaga ggaattttca ggcggcttaa ttccatcacc
     9121 tgatcctgca tggcgcggat atcctgacgg gttacggtgt tgaaaatagc cccaacctga
     9181 ccgtctttga tcatctcgcg gatcgcctct ttcgggttat ccgggccgac gctgattaag
     9241 cgcagctgac caattttctc atcaactgtc attttcttaa gcagttcggt gacgaacgca
     9301 tcccgcgctt caggcgttaa tggatggtta ccgaataaat catccgccag tgctggctgc
     9361 agggccagac tcaccgcgat tcctactgaa catagccatt tcatatggat ttttcttcct
     9421 ttgttgcccg acgtggcagc gaaaatggtg caaaaaccgt agtttgccat aagcatgatg
     9481 gagagagaaa aagaatgctc agtttattgt ctgaattttc aaaatattca ctcgctgaat
     9541 tgtcatacaa ggcgctattc tagtttgtga tgttttttcg ccaccacaag gagtggaaaa
     9601 tgtcttccat gacaacaact gataataaag cctttttgaa tgaacttgcc cgtctggtcg
     9661 gtcattcaca cctgctcacc gatcccgcaa aaacggcccg ctatcgcaag ggcttccgtt
     9721 ctggtcatgg cgacgcgctg gctgtcgttt tccctggctc actactggaa ttgtggcggg
     9781 tgctgaaagc ctgcgtcacc gccgacaaaa ttattctgat gcaggctgcc aatacaggcc
     9841 tgaccgaagg atcgacgcca aacggtaacg actatgatcg cgatatcgtg atcatcagca
     9901 ccctacgtct cgacaagctg cacgttctcg gcaagggcga acaggtgctg gcctatccgg
     9961 gcaccacgct ttattccctg gaaaaagccc tcaaaccgct gggacgcgaa ccgcactcgg
    10021 tgattggttc atcgtgcatt ggcgcatcgg ttatcggcgg tatctgtaat aactctggtg
    10081 gttcgctggt gcagcgtggc ccggcgtata ccgaaatgtc gttattcgcg cgtataaatg
    10141 aagatggcaa actgacgctg gtgaaccatc tggggattga tctgggcgaa acgccggagc
    10201 agatccttag caagctggat gacgatcgca tcaaagatga cgatgtgcgt cacgatggtc
    10261 gtcacgccca cgattatgac tatgtccacc gcgttcgtga tattgaagcc gacacgcccg
    10321 cacgttataa cgccgatccg gatcggttat ttgaatcttc tggttgcgcc gggaagctgg
    10381 ccgtctttgc agtacgtctt gataccttcg aagcggaaaa aaatcagcag gtgttttata
    10441 tcggcaccaa ccagccggaa gtgctgaccg aaatccgccg tcatattctg gctaacttcg
    10501 aaaatctgcc ggttgccggg gaatatatgc accgggatat ctacgatatt gcggaaaaat
    10561 acggcaaaga caccttcctg atgattgata agttaggcac cgacaagatg ccgttcttct
    10621 ttaatctcaa gggacgcacc gatgcgatgc tggagaaagt gaaattcttc cgtccgcatt
    10681 ttactgaccg tgcgatgcaa aaattcggtc acctgttccc cagccattta ccgccgcgca
    10741 tgaaaaactg gcgcgataaa tacgagcatc atctgctgtt aaaaatggcg ggcgatggcg
    10801 tgggcgaagc caaatcgtgg ctggtggatt atttcaaaca ggccgaaggc gatttctttg
    10861 tctgtacgcc ggaggaaggc agcaaagcgt ttttacaccg tttcgccgct gcgggcgcag
    10921 caattcgtta tcaggcggtg cattccgatg aagtcgaaga cattctggcg ctggatatcg
    10981 ctctgcggcg taacgacacc gagtggtatg agcatttacc gccggagatc gacagccagc
    11041 tggtgcacaa gctctattac ggccatttta tgtgctatgt cttccatcag gattacatcg
    11101 ttaaaaaagg cgtggatgtg catgcgttga aagaacagat gctggagctg ctacagcagc
    11161 gcggcgcgca ataccctgcc gaacataacg tcggtcattt gtataaagca ccggagacat
    11221 tgcagaagtt ttatcgcgag aacgatccga ccaacagtat gaatccgggg atcggtaaaa
    11281 ccagtaagcg gaaaaactgg caggaagtgg agtaaaaatt acggatggca gagtatcgcc
    11341 atccgaattc acttaatcgt tctgtgccgt ctgccccgcc gccgccattt gggcggcttt
    11401 ttgtttttta tagcttaacg ctgctgccgg aacaggcatc actttaccgg tttcaatcca
    11461 ggtacgcagg cggctggcat cggcaaaatg ggtgtatttg ccaaacgcat ccatcactac
    11521 cagcgccacc ggtttattat tgataaccgt acgcatcacc agacaatggc ccgccgcatt
    11581 ggtaaagccg gttttggtta gctggatatt ccagttatcg cgatacacca gatgattagt
    11641 attgcggaac ggcagcgtat acgtcgggtt agagaaggtt gccatatctt cccgggtagt
    11701 acttaactgc ccgatcaacg gatattgttt gctggcaatg agcagtttgg ttaagtcacg
    11761 ggcggttgaa acgttatgca ccgacaatcc ggtaggttca acaaagcgcg tgttgttcat
    11821 tccgagcgat ttcgctttcg cattcattgc cttaataaag gctttgtaac caccgggata
    11881 atggtgcgca aggcttgccg ccgcgcggtt ttctgaagac atcagcgcca gcaacagcat
    11941 atctttacgg ctgatttcgc tattcagtcg tacgcgcgaa tagacccctt tcatctccgg
    12001 cgtctggctg atatccactt ttagtttttc atccagcggc agtcgtgcat ccagcacgac
    12061 catcgcagtc attaatttgc tgatagacgc aatcggacgc accagatccg ggtggttcga
    12121 atagatcact ttgttggtat tcagatcaac aatcatcgcg ctaccggagg caatttccgg
    12181 ttgtgaagcg gtggtagcgg ctgccgtttt cgcaacggcc tgcggtgcca aaggcacagc
    12241 cagcatcagg gccaggctaa ataaagaaac tcgaaatttc ggcatgatga gcattcagat
    12301 agtggttcac gcgcacgggt tgcgcaccgc cggagtaagg atttactgag gctagcgacg
    12361 ccatcataac gagcaaaaag tgcgatcgtc aaaggagaat cgtgaggaaa tgctgcattg
    12421 ctgacattta cgccagcaat gcaacgtcaa agaactttct agaacaaacg ataaccgtag
    12481 ccccatagta taacggtaag ggcgagcagc acttccagta ccagcacgcc aatcgccagc
    12541 gtcgaactgg agaagctaag gccttcctct ttgttgatat tcaggaagct cggaatacca
    12601 aggtaaagta ggtaaccggt gtaaaacagt gccaccgtgc cgaccagcgc acacaaccag
    12661 accagcggat aaagcgccac cagaccactt aaaaacagcg gagttgcaac gtagcccgcg
    12721 aagaccatgc agtgcgcaag tgacggacgc tgcggataat tacgcgccat ccaccagatg
    12781 acccgcccca tcaccgcgac cccagcgagc ataacgccat aaaacaggac agccagcgcc
    12841 agtccggtaa accaggataa cttcaggata gtgccatcgc caaaattcca gccaatctgt
    12901 gtagtgccaa tgaaggcgca aatcaccgga atcgccgcca tcagcaaaac gtggtgggtg
    12961 taatggtgag aaatcgtttc gttttcgcga ttaatcacct gcatttcacg atcgggatgg
    13021 gaaaacagtc cccagacatg gctcataccg cccccttgtt gtgagttcat gaacctgaca
    13081 gttcaagtat aagtcagctt gtgattattt tttgttcgcc catgtaattt gcaccgtctg
    13141 atgtacgtcc tttttcagcg tgaatgattc acagggtgta tgcttacagg caaccaaagg
    13201 gagacagact ggcctatgga tctcaataca ctcatctcac aatatggtta tgccgcgctg
    13261 gttatcggta gcctggcgga aggtgaaacc gtgactttgc tgggaggcct tgcggcacat
    13321 caggggctat taaagttccc gctggtggta ctttctgtag cgcttggcgg catgattggc
    13381 gaccaggtgc tctatctgtg cgggcggcgg tttggcggta agctgttacg ccgtttctct
    13441 aaacatcagg ataaaattga gcgggcgcag aaactgatcc aacgccatcc gtatctgttt
    13501 gtcattggta cgcgctttat gtatggcttt cgggtgattg gcccgacgct gattggtgcc
    13561 agccagctgc cgccgaaaat ctttctgccg cttaatattc tcggcgcatt tgcctgggcg
    13621 ttgattttta ccactatcgg ttacgctggt ggtcaggtga ttgcgccgtg gttgcacaat
    13681 ctcgaccagc atttgaagca ctgggtctgg ttgattctgg tcgtggttct ggtggtaggc
    13741 gtgcgctggt ggctgaaacg acgcgggaag aaaaagccgg ataatcaggc gtaaaaccat
    13801 tgccctggat aaggcgtgcg cgccgcatcc gacatccggt tcaccatgcc tgatgcggcg
    13861 cttgtgcgtc ttatcatgcc taccaaaatt ttacccattg aaatatccac acacctcact
    13921 gcgctattct gggttgaact gcggattcgc caacataaag ccgccatcca ctatcaacga
    13981 ctgcccggtg gtgtaatttg cgccctccga acaaagccac gccaccaggc tggcaatctc
    14041 atgcgttgcg ccaaaacgcc gcagaggaat cgaaggctcc gcgtcgggct tcacgtcgct
    14101 atcatccatg ccgttcattg gcgtagcgat cgccccaggt gcgactgcgt tcaccaaaat
    14161 cttatgcctg accaactcca gcgccattgc tttggttaac ccgccgagcg catgtttagc
    14221 ggctgtgtag gcgctggcat ccggcagcgg cgtatgttca tgtaccgacg taatgttgat
    14281 gatgcgacca ccctgtcctt gtttcaccat ctggcgggca gcaatttgcg agcataaaaa
    14341 tgcaccatcg acatcaacgg taaaaatctt gcgccactca tcgaaagcca tatcaagaaa
    14401 cggcactttg gtcattgcgc ccgcattatt caccagcaca tcaatacgcc ccagccgttg
    14461 aataagtttc tccagcgcct gcgcaccttc tggcagattg ccgagatcca gttgcacgat
    14521 ctccgcacgt acgccgtggc taactacctc acgcgcggta tcttttgccc cttcttcatc
    14581 tgagtgccag gtaatgccaa tatcaaaccc ttgctgcgcc agtaataacg cgcactcttt
    14641 gccgatcccc gaatcggagg cggtaataat cgctacctgt gccatcgagt tctccactta
    14701 acgctgaata aacgttaagt atagaagtca catatcatca gcgtttgtaa cccccgccca
    14761 acgcaccagt gagttgaatg gaggcatcca gccactgccc ttgcaataac aggccattgg
    14821 cccgctcacg cagcgcgggg attctggctt cgctgacgcg ggaaccagca atgatgcccg
    14881 cgttaaagcg ggcctgcgca agacctacca cacgcaaggc atcgcgctca atttgcgcct
    14941 gatgctggtt tttctccgcc agtgtctgaa cctgactggc tgcccgcgcc acgtcattca
    15001 ccgcttcaac caccgctttg ttgtagctgg cgatagacaa gttgctttcg gcttttgcga
    15061 tatcgagatt ggcgttaaga cgaccactat cgaaaatggg tagcgtcagg cctgccgtaa
    15121 cgcccatttg ctgcgcggaa tgacggaaca gatcgcttaa gtgcaacgca tcctgttgca
    15181 ggaaggccat caggttgatg tcaggataaa atgccgcttt tgccgcatca atggtgctta
    15241 gcgatgactc aacgtaccag tgcgccgcct gcaaatctgc ccgccgggcc agtaaggagt
    15301 accccagttc atcaggaagc tggcttgcca ctttcggcaa cgccaccggt ttaagcgtca
    15361 atgacttagt ctggtgattt gtgagtgcaa ttaaccgtgc ctcaataatt ttcattttcc
    15421 ccgcgacatc gttgagctgc tgccgggttt tgctggcatt aatatcggtt tccacacctt
    15481 caactgaaga agtaatcccg ttctgatata gctggcgatc ggtcgcgata atggtgttct
    15541 gctctttttc gatttgctgc aagaccgtgt ttaacgccgc ctgggtttgc cactcccagt
    15601 acaggcgggc tacgctgcca gccagcaatt ggcgggtttg ctcgcgttcc gccgcccgtg
    15661 ctttaaccgt acccaggcgg gcagtaacct ccgcccgatt ctttccccag atatcgagat
    15721 gccagcccgc cgttaagcca aaagttccgt tggtgtacca cggaccggtc gtacctgcgg
    15781 ccggatcgtt cagagcaaac ggccccatta gcccttctgc cgacattttt tgccgctcca
    15841 tatccgcaga aaagtcgatc tgcggaccat cctgagtagc aactgccttc gcctgggctt
    15901 cagctaactg aatgcgctgt tcagccacct gcatatccgg tgcgttctgt agtgcattgt
    15961 taattaagga agtgagttga ttatcgtgat actccagcca ccattggctg tctggccaac
    16021 catttttcag cgccgtgggt aatgcggtgt caacttgtgc agcgggcgtt tgctggctta
    16081 acgcctggcg ggtttcatgc ataggcgcac acccggccag catcagtaac agcggaaaac
    16141 aggcgatggc tggataaaag gaatcacgat tcatggggga ataatcaggt aagaaaaggt
    16201 gcgcggagat taccgtgtgt tgcgatatat tttttagttt cgcgtggcaa tacatcagta
    16261 gcaataaaac gacatatcca gaaaaatata cactaagtga atgatatctt ccgatttatc
    16321 ttaatcgttt atggataacg gcaaagggct tcgttttttc ctatacttat tcagcactca
    16381 caaataaagg aacgccaatg aaaattatac tctgggctgt attgattatt ttcctgattg
    16441 ggctactggt ggtgactggc gtatttaaga tgatatttta aaaatcagat atgacatata
    16501 agttgtcagg tcaggaacta gatattttgt gtacttgatt tgcaaacagc tccggtgttg
    16561 gtggcgtcaa aatcgctgaa gcgttccccg aaggccgggg cagcccacat ggatgtgggc
    16621 tgagggcgcg tcttacaagg acgtttcctc gcgcccgacc cggtagccga aggggataag
    16681 ctgaaggtac cgcgcagcgg cgattttgtt cgccagagcc cgggggtgca gggggaggag
    16741 gcgattgtcc tccccctgcg cgctccttgc gccagtggca atatgtggct tagctcatga
    16801 aaggagcgca acaagatgat gaagctgaat aagcgattca ttgtttctga taaactgcga
    16861 ttccactatt atcgcagctt accgatatca attgcctgaa tagcccttgc aatatcaggg
    16921 gaattattca acacacgaac atgctgaaat aattctgttg cttcatcgta ttctttacgc
    16981 aaataactca accactgttt aatccgcgct acgtgatata acccggtatc gccctgcttt
    17041 tccagacggg tatatttttg cagcaaagca accacctccg gccacggcat tcgcggttcg
    17101 ttatatttta ctacccggct caggttggga atattgagcg ccccgcgacc aatcatcact
    17161 gagtcgcagc cgctgatcgc catgcattct tgcgcgctct gccagtccca gatttcgccg
    17221 ttggcaatca ccggaatatt cagccgctgg cgaatctcgc caatcgcctg ccagtcaata
    17281 tgctccgcgc ggtaaccctg ctcttttgtc cgtccatgca ccaccagctc cgtagcacca
    17341 gcctgttgaa ccgcatcggc gatttcaaat ttcttctcac cgctgtccca gcccagacgc
    17401 actttcacgc tgacgggcaa atgcgccggt acagcttcac gcatcgcttt tgcaccctgg
    17461 tagatgagtt caggatcttt gagtaacgtc gccccaccac cgctaccgtt aaccgttttc
    17521 gacgggcagc cgcaattgag atccacgccc caggaaccta attccaccgc acgggcggcg
    17581 ttctctgcca gccattgtgg gaactgaccc aacaactgca cgcgcaccag cgtaccggat
    17641 ggtgtccggc tggcgttttg tagctcaggg caaatgcgat gaaagacttt caccggcagc
    17701 agttgatcca ccacgcggac gaactcggtg atgcacagat cgtagtcgtt aacttcggtc
    17761 agcaattcac gcaccagaga gtcaagcact ccctccatcg gagccagtaa cacacgcata
    17821 tcatcacccg caaaaaaatg aggcgctatg ttagcgcctc ctgtgtttgc agacaaactg
    17881 acttactgat taattcgggc aagattttta ccctcaccct aaccctctcc cgaaggggcg
    17941 aggggaccgc ccgtgcgcct gattgggtga agaagtgctt agcgtggttg caaacagtta
    18001 tcttctttcc agccgtacaa ccactccatg ccgcgataaa attctgcctg cgatttccct
    18061 ttccacagca gattacgtac ctggcgttct acccctgcgg cgtggtaaac ccgccccatt
    18121 tcacgggtcg accagacgat gcgcgcggtg cgcggaattc gtaccgactc atacagcgca
    18181 aaggcttgcg ccgcatcacc ctcacactgc gccagcgcct tacccagcgt tactgcatct
    18241 tccagcgcca tacaggctcc ctgcgccata tattgcgcca ccggatgggc cgaatcgccc
    18301 acaagcgtta tgcgcttcgt tccccacttc gccaccggtt cacggtcagc ggttgaccag
    18361 cgtcgccatg aagtcggttt atccaacatc tggcgcggac gggggtgaat cccttcaaag
    18421 taagagagca cttcctcttt actaccatcc ttcacgcccc actcttcctg ctggcggctg
    18481 tggaatgtta ccaccaggtt atactgcttg ccgccgcgca aagggtaatg caccaagtga
    18541 caatgcggcc ccgcccacag taccggtgcg ttaatgcgca aatcttccgg catatcggca
    18601 cgatcgataa ccgcacggta gacaacatgt ccggtgacac gcggcgcatc gcccaataag
    18661 ctctggcgca ccactgactt cacgccgtca cagccaatca gaatatcgcc ggtccagcta
    18721 ttgccgcgat cgtcaaacac ggtgacatcg tcttccgtct cgcgaatatc cacgacgttg
    18781 gtggaggtgt gatattccac gtccggatgc gtcagtaccg cttcccacac cgaggcgtga
    18841 atatcaaccc ggtggatcac cgcgtaaggg ccgccgaaat ggtcacgaaa ggcctgcccg
    18901 gtttcaatgc acaccacttc ttcggcgttc accgcatcca tcatggtaat gtgatcggta
    18961 aacaccgcgc gctggcgggc aacttcgccg acgccgaggc tgtccagcgc cgaaaacgca
    19021 tttggcccca actggatgcc cgcgccaatc tcaccgatct catgcgcctt ttccagcagc
    19081 accactttga tccccaggcg ggccagtgac agcgcagttg ccgcaccgcc gatcccgcct
    19141 cccacaatta ctgcgcgtgt cactttcgtc attatagttc tcctggtcag gctgaaattt
    19201 tatcttgttg attttctggc gctgcggcaa tgaacgctgg cagctgtgtg caggcgtcgt
    19261 acaccgcttt gcagcgtgga tacccgctca aatcgcagtt catgcgtagc gcgttcgccc
    19321 actgcgggac aaggcagcaa tctgccagcg tcggcgtctc gcccacacaa aattgccccg
    19381 actggttctg gcgcaacagt tgttccaccg cgcttaaccc ttgctggatc cagtgcgcat
    19441 accaacgttt tttttcttct tcactgacgt tcagttcttc ggtcaggtag cgcagcacgc
    19501 gcaagttatt caatggatga atatcacaag cgatggcata gacaatttcc agcaccttgt
    19561 tgcgggccgg ctcttcctgc ggcaccagcg gaacttgcgg gtagtgcctc tccagccagt
    19621 caataatcgc cagcgattgt ccgagtgatt cgccctcgtc agtcagcagc gtcggcacca
    19681 gcccgaccgg attcatccgc cgataggcca gttcattttg ctgaccaata cggatattca
    19741 ccccttcggt ctggtaatta atgcctttca gcgccagcgc aatgcgtacc cggtacgacg
    19801 ctgaactatt aaaaaaactg tacagcttca tcatgcacct cagacaatct tcaccgcgat
    19861 aggcgttaag ccctcgacgt tgcctgtgat cacatcacct ttcactaccg ggccaacccc
    19921 ttccggcgta ccggtgaaga tcagatcgcc cggttgcagc tcaaagaagc ctgacagata
    19981 gcagatggtt tcattcaccg accagatgag atggcgaata tcgctgcgct gatgatcttc
    20041 gccgttaacc tgtaaccaga ttggcgcatt atcaataccg ccgatatcgc gggctttgtg
    20101 cagcggcgcg atgggcgctg agagatcaaa cgctttaccg atttcccacg ggcggcccat
    20161 ctggcgcatt tccatttgac ggtcacgccg agtcatatca agacccgtgg cataacccca
    20221 gacatattca tgggcgtgtt ccagcgggat atcgctgcct tttttaccaa tcgccaccac
    20281 cagttcaatt tcgtaatggt agttatcggt ctgtgccgga tacggcagcg ccagcgtttc
    20341 accagcggca accggaacaa cggcatccgc cggtttgcag aagaagaatg gcggttcacg
    20401 atccggatca aagcccattt cgcgggcgtg agcggcatag ttacgtccga cgcagtaaac
    20461 gcggcgcacg gggaattgtt catcactgcc aacaaccggt acggtaacgg gcgcttgcgg
    20521 ttcgaatacg tattgagtca tttttctctc ctaaattaat aacgcgcttc gcggaacagc
    20581 cccagggctt cttgtaccgg tctgtccgaa aaactaaata aaacggaatc ttgcgtggtc
    20641 tggaacgaca cgccatgcca ggtcggcacc acgaagatat cttttgcact aaagctgaag
    20701 gtttcattgc cgatgatgac ctgcccgcta ccttccacca catggtagat ggtgctgtca
    20761 gtggtacgcg caacgcgtga ggcaaaccct ttcggcagca gttgcaggaa agcgcccatc
    20821 gacggcatcg gatagccgcc ggtgaccggg ttgacgtagc gcattttgta gccatcccac
    20881 tcgtcggcat cgcccagtcg ggttaaatcg tgcagcactt cgcggctgcg gtcataacga
    20941 tagttaaaga tcggcgagga gttccccgtc tgatgccgca gcggcaacat attggcagcg
    21001 taacgcggca gataatctcc ctctttacgc gttaccggtt gttgctcttc cggataatct
    21061 tcggcaaaac cgcagcccag aatattcact aatggcagat ccagcccatc gagccagata
    21121 accggttcgt cgccagggtt accgtgatcg tgccagcgcc actgcggggt caggataaaa
    21181 tcgccttcat tcattggcgt gcgttcgcca tcaacggcgg taaatgcccc tttgccttca
    21241 acaataaaac gcagcgccga ctgattatga cgatgactcg gtgccacttc gcccggcatg
    21301 atcagttgca aaccggcata taaagtcgcg gtaatggaag actgcccgcg cagcgccggg
    21361 ttttccagca ccagcacgcg gcgcacggcc tctttcgcac caatcaggcc gccgctctcc
    21421 agtagcagcg ggcggatctc ctgataattc cagtacgctg gcgcacagtt agcgttgggc
    21481 gttttcggca ccagatggtg cagtgactcc cacagcgggg tcaggttttg cccggaaata
    21541 tgctgataaa attgctcgcg gctattttga ttgttatcag tcattgagat gctccttatt
    21601 cgttcacaac gcctgcgcga ggcagtgagt cagggacaga ggtcgactgg cgaaccacgg
    21661 taatcagcag ggttaacatc accgcactga tcgccgcagg cacagcgata atgaaaaaca
    21721 gggtgtcgaa agagaaattc atcgccatca tcacgccgcc agacagcgaa ccaacaatcg
    21781 cgccgcagcg gccgatagca tttgaccagc tcacgcccgt ggctcgactt tgtgttggat
    21841 aaagtgttgc ggtcagcgca ttcagcccca cctgcgatcc gctaatgcca atgccagtgc
    21901 cgaaaatcgc cagcgccatc aaccataatc catcctggct taagccaatc atcacgatgc
    21961 atatagcgcc tatggcatag ctcagcgtca gcacgcggaa aggattgaac ttatccatca
    22021 gcacacccag cgccagcgcg cctaacgtac caccaatctg gaacgcggcg gtgacccagg
    22081 acgcgtgttg caggtcgatg ccgcgatggt tcagtagcgt cggcatccag cttgagagca
    22141 ggtaaataat cagcaggctc ataaagaaca ctacccacag catcaacgtg atgggtaact
    22201 gacgtcccat aaacagttga cgaatgctgc cttttgtaac ggaggctgat tcaatgagat
    22261 aaaagtgggt atcgacataa cgctcgcggg tgatggcgct gacggttttc gcaatcaccg
    22321 cctgcggtag ctggcggcgc acctgccagc gtggcgattc cggcagaact accagcagtg
    22381 cgacaaacag catcagcggt aacacgcccc ccaacaccag aatgccgtgc cagccaatca
    22441 ccggcaccag ctgcgcactg acaatcccgc caaacgccga acccagcgta aagccgcaaa
    22501 acatcagcgt caccagcgcg ccgcggcggc gggcaggcag atactccgag gtcatggtga
    22561 tggtgttcgg catcgcgccg cccagaccca ggccggtgag aaagcgcagg aagaccagcg
    22621 tttgcagatc cggtgagaaa gccgaggcga ggcttaacgc gccaaacaaa aagacgcaca
    22681 gctcaatcac ccgcttgcga ccaaagcgat cggacaacgg gccacaaagc agtgcgcctg
    22741 cggttaatcc tagcagaccc gcgccaaaaa gcggggcgag atcgcctgcg gtgagttgcc
    22801 agtgagtgcg aatatcaggg gcgataaatc cgatagccgc ggtgtcaaaa ccatcgagca
    22861 ttaccaccag aaaacagcag atgataacgc gccactgcat cttgccgacc ggagcagcat
    22921 cgataagtgc ttgtaaatca cgtcgttgag tcatagtaaa tgcctcagtg caggtaaggt
    22981 agatgtttct tttgttttgt atatgttgcg atattgtgat taaagagatg agatgtacaa
    23041 tggcatttcg ggcagagaga taaccttgag gttatggcaa atggcgaact gggcgcaaaa
    23101 attaaaacta catcaccttg aaatgctggt ggcgctgggt gaacagggca atctaaccca
    23161 tgtcgcacga atgatgaata tcacccaacc tgcgctttcg aaatggttgt cacaactcga
    23221 agatgagatt ggaatcacac tttttgagcg gcacagcaaa ggattacgtc cgactgaagg
    23281 cggcaaattg ttattgcaac atgcccaacg gttaatcaat gacatggagc gatcgcaaca
    23341 tgaactggcc cgttttaaac aaggtggact ggtcggcagt ctgaaaattg gctgttctcc
    23401 cgtcgccact gactgcgtat cccaggcgat tttgcatttg cttacgaaga tgccaactct
    23461 acacctgaat atcgaagaga aggtgatgac cccattgctg caagatctat tggccggttc
    23521 tgtcgatgtg gtcgttgggc gaattggcgg tcgcgcattg cagctgccac tgaattatca
    23581 ggtgctgtac accgagccag tatgctttgt cgcccgccct gagcacccac tggcgaaata
    23641 cgccaccctc agctggaatg atcttgccaa ctggcgctgg atcgtctggc cgacgggtac
    23701 ccccattcgc attagtattg ataatgcgct ggtagaccac ggcgtaatgc tgccagaaaa
    23761 cacgattgag tcggcgtcga tgaacgtcag taccaacctg ttacaaagca gcgatatgat
    23821 ctctattttg tcattacgcc tggcgcaacg ttatgccagc cagggacaac tggtgatact
    23881 caacctgcca aagatagaac agaaaggtag cgtcggtgtg ttctggcgca ataacgaaac
    23941 gccttcttat gctttaagtc agtttctgca ctttttacag cagaattagt gtgctgaacg
    24001 gggcgctcgc ccgaggttac ctccctaaaa ttcaactatg ctgagaattc ttaatgtgat
    24061 cggtagcacg ttttacgaat taattgtatg atgaatccat ctcatctggg gtgttgatta
    24121 tgagtaagac actgaacatt atctggcaat atttacgcgc tttcgtcctg atttatgcct
    24181 gcctgtatgc aggcattttc attgcttccc tgctaccggt aaccattccg ggcagcatca
    24241 tcgggatgct gatcctgttt gtcctgctgg ccttgcaaat tcttccggcg aaatgggtca
    24301 atccggggtg ctacgtactg attcgctata tggcgctatt gtttgtgccg attggcgtag
    24361 gcgtcatgca atattttgat ttgctccgcg cacagtttgg cccggtagtg gtttcctgtg
    24421 caatcagtac gctggtggtt tttctggtgg tgagctggag ttcgcaactg gtacacggtg
    24481 aacgtaaagt cgtaggtcag aaaggatcag aagaatgatg gcgaatatct ggtggtcatt
    24541 accgctgacg ttgattgtct tttttgccgc ccgcaaactg gcggcacggt ataagtttcc
    24601 cctgcttaac ccgttgctgg tagcaatggt ggtgatcatt ccttttttaa tgctgaccgg
    24661 catctcttac gacagttact ttaaaggtag cgaagtgcta aacgatctgc tgcaaccggc
    24721 ggtcgtcgcg ctggcctatc ctttatatga gcagctacac cagatccgcg cgcgctggaa
    24781 atcgatcatc accatctgtt ttatcggcag cgtggttgcg atggtgacgg ggacttccgt
    24841 ggcattgttg atgggcgctt caccggaaat cgccgcgtca atcctgccga aatcagtcac
    24901 cacgcctatt gcaatggcgg ttggcggcag tattggcggt attccggcaa tcagcgcagt
    24961 ttgcgtgatt ttcgtcggca tcctcggcgc ggtatttggc catacattgc ttaatgcgat
    25021 gcgtattcgt accaaagctg cgcgcggtct ggcaatgggg actgcctcgc acgccctcgg
    25081 tacggcgcgc tgcgccgagc tggattatca ggaaggtgca tttagttcgc tagcgctggt
    25141 gttatgcggg ataattactt cgctgatcgc accgatcctt ttcccgatta ttctggcagt
    25201 aatgggctaa aatttgcgat gcgtcgcgca tttttgatgt aagtttcacg cgttgcataa
    25261 ttaatgagat tcagatcaca tataaagcca taacgggttc gtaaactgtt atcccattac
    25321 atgattatga ggcaacgcca tgcatccacg ttttcaaacc gcttttgccc aacttgcgga
    25381 taacttgcaa tctgcactgg aacctattct ggcagacaag tacttccccg ctttgttgac
    25441 cggggagcaa gtctcatcgc tgaagagcgc aacggggctg gacgaagacg cgctggcatt
    25501 cgcactactt ccgctggcgg cggcctgtgc gcgtacgcca ttgtcgaatt ttaatgttgg
    25561 cgcaattgcg cgcggtgtga gcggaacctg gtattttggt gccaatatgg aatttattgg
    25621 tgcgacaatg cagcaaaccg ttcatgccga acaaagcgcg atcagccacg cctggttgag
    25681 tggtgaaaaa gcgcttgcag ccatcaccgt taactacacg ccttgtggtc actgccgtca
    25741 gtttatgaat gaactgaaca gcggtctgga tctgcgtatt catctgccgg gccgcgaggc
    25801 acacgcgctg cgtgactatc tggcggatgc ctttgggccg aaagatctgg agattaaaac
    25861 gctgctgatg gacgagcagg atcacggcta tgcgctgact ggtgatgcgc tttctcaggc
    25921 ggcgattgcg gcggcaaacc gttcgcacat gccttacagt aagtcgccaa gcggtgtcgc
    25981 gctggaatgt aaagacggtc gtattttcag cggcagctac gctgaaaacg ccgcattcaa
    26041 cccgactctg ccaccgttgc agggagcgtt aattctattg aatctcaagg gttatgatta
    26101 cccggatatc cagcgcgcag ttctggcaga gaaagccgat gcgccgttga ttcagtggga
    26161 tgccacctcc gcaacactga aagctctcgg ctgtcacaat atcgaccgcg tgcttctcgc
    26221 ttaagcctgg tgccggatgc ggcgtgaacg ccttgtccgg cttgccagcc ctctcctggt
    26281 gtcgaaaatc ccggcaaaca gtttgtcgtt tcttgcgcaa aaccagcggg taaagtagcc
    26341 tgatggaaat tttccttaga tcgagtctcc tgcatgttaa agcgcgtgtt cctcagcctg
    26401 ttagtcctga tcggcttgct gctgttgact gtgctcggcc tcgatcgctg gatgagctgg
    26461 aaaaccgcgc cttatatcta cgacgaattg caggatctcc cctaccgcca ggtcggtgtg
    26521 gtgctcggaa cagcaaaata ttatcgtact ggcgtaatta atcagtatta tcgctaccgc
    26581 attcaaggag cgattaatgc ctataacagc ggtaaggtaa attatctatt actgagcggc
    26641 gataacgcat tgcaaagtta taatgagccg atgaccatgc gcaaagattt aatcgctgct
    26701 ggtgtcgacc catcagatat tgttctcgat tacgcaggct ttcgtacgct ggattccatc
    26761 gtgcgtacac gcaaagtttt cgatactaat gatttcatta ttatcaccca acgtttccac
    26821 tgtgagcggg cattatttat tgcgctgcat atggggattc aggctcagtg ttatgccgta
    26881 ccgtcaccga aagatatgct gtcagtacgt attcgtgaat ttgccgcccg tttcggtgcg
    26941 ctggctgacc tttatatttt taaacgtgaa ccgcgttttt tagggccgct ggtccctatt
    27001 ccggctatgc accaggtgcc ggaagatgcg caggggtatc ccgccgtcac acccgaacag
    27061 ttacttgaat tacaaaagaa acaaggaaag tagttatgga tgtacagcag ttttttgtcg
    27121 ttgccgtttt tttccttatc ccgatatttt gtttccgcga agcatggaaa ggctggcgcg
    27181 caggcgcgat tgataaacgg gttaaaaatg caccggaacc ggtgtatgtc tggcgagcaa
    27241 aaaatcccgg actcttt
//
LOCUS       DAZKFZ010000050        27057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000050 DAZKFZ010000000
VERSION     DAZKFZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.050"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            136..2316
                     /gene="katG"
                     /locus_tag="ACHOQW_001887"
     CDS             136..2316
                     /gene="katG"
                     /locus_tag="ACHOQW_001887"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP9572972.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            2410..3315
                     /gene="yijE"
                     /locus_tag="ACHOQW_001888"
     CDS             2410..3315
                     /gene="yijE"
                     /locus_tag="ACHOQW_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP9572973.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(3342..3959)
                     /locus_tag="ACHOQW_001889"
     CDS             complement(3342..3959)
                     /locus_tag="ACHOQW_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP9572974.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(4233..5336)
                     /gene="gldA"
                     /locus_tag="ACHOQW_001890"
     CDS             complement(4233..5336)
                     /gene="gldA"
                     /locus_tag="ACHOQW_001890"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP9572975.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(5347..6009)
                     /gene="fsa"
                     /locus_tag="ACHOQW_001891"
     CDS             complement(5347..6009)
                     /gene="fsa"
                     /locus_tag="ACHOQW_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9572976.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(6021..8522)
                     /gene="ptsP"
                     /locus_tag="ACHOQW_001892"
     CDS             complement(6021..8522)
                     /gene="ptsP"
                     /locus_tag="ACHOQW_001892"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9572977.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            8831..9910
                     /locus_tag="ACHOQW_001893"
     CDS             8831..9910
                     /locus_tag="ACHOQW_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP9572978.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            9925..10245
                     /locus_tag="ACHOQW_001894"
     CDS             9925..10245
                     /locus_tag="ACHOQW_001894"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9572979.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            10296..12593
                     /locus_tag="ACHOQW_001895"
     CDS             10296..12593
                     /locus_tag="ACHOQW_001895"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9572980.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            12559..13437
                     /locus_tag="ACHOQW_001896"
     CDS             12559..13437
                     /locus_tag="ACHOQW_001896"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP9572981.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            13439..13780
                     /locus_tag="ACHOQW_001897"
     CDS             13439..13780
                     /locus_tag="ACHOQW_001897"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9572982.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13767..14618)
                     /locus_tag="ACHOQW_001898"
     CDS             complement(13767..14618)
                     /locus_tag="ACHOQW_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9572983.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(14833..16566)
                     /locus_tag="ACHOQW_001899"
     CDS             complement(14833..16566)
                     /locus_tag="ACHOQW_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9572984.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(16749..19400)
                     /gene="ppc"
                     /locus_tag="ACHOQW_001900"
     CDS             complement(16749..19400)
                     /gene="ppc"
                     /locus_tag="ACHOQW_001900"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP9572985.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(19752..20903)
                     /gene="argE"
                     /locus_tag="ACHOQW_001901"
     CDS             complement(19752..20903)
                     /gene="argE"
                     /locus_tag="ACHOQW_001901"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP9572986.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            21057..22061
                     /gene="argC"
                     /locus_tag="ACHOQW_001902"
     CDS             21057..22061
                     /gene="argC"
                     /locus_tag="ACHOQW_001902"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP9572987.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            22069..22845
                     /gene="argB"
                     /locus_tag="ACHOQW_001903"
     CDS             22069..22845
                     /gene="argB"
                     /locus_tag="ACHOQW_001903"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP9572988.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            22906..24279
                     /gene="argH"
                     /locus_tag="ACHOQW_001904"
     CDS             22906..24279
                     /gene="argH"
                     /locus_tag="ACHOQW_001904"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP9572989.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            24546..25463
                     /gene="oxyR"
                     /locus_tag="ACHOQW_001905"
     CDS             24546..25463
                     /gene="oxyR"
                     /locus_tag="ACHOQW_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP9572990.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(25446..26846)
                     /gene="sthA"
                     /locus_tag="ACHOQW_001906"
     CDS             complement(25446..26846)
                     /gene="sthA"
                     /locus_tag="ACHOQW_001906"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP9572991.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         6092 a         6844 c         7574 g         6547 t
ORIGIN      
        1 acgatcaatt tgatctacat ctctttaacc aacaatatgt aagatctcaa ctatcgcatc
       61 cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg taacactgta
      121 gaggggagca cattgatgag cacgtcagac gatatccata acacgacagc cactggcaaa
      181 tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac cactcgcgac
      241 tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg ttctaacccg
      301 ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta cggcctgaaa
      361 aaagatctga aagccctgct gacagaatct cagccgtggt ggccagccga ctggggcagc
      421 tacgccggtc tgtttattcg tatggcctgg cacggcgcgg ggacttaccg atcaatcgat
      481 ggacgcggtg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc ctggccggat
      541 aacgtaagcc tcgataaagc gcgtcgcttg ttgtggccaa tcaaacagaa atatggtcag
      601 aaaatctcct gggccgacct gtttatcctc gcgggtaacg tggcgctgga aaactccggc
      661 ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga tctggatgtt
      721 aactggggtg atgaaaaagc ctggctgact caccgtcatc cggaagcgct ggcgaaagca
      781 ccgctgggtg caaccgagat gggtctgatt tacgttaacc cggaaggccc ggatcacagc
      841 ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct tcggcaacat gggtatgaac
      901 gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc tgggtaaaac ccacggtgct
      961 ggtccgacat caaatgtagg tcctgatcca gaagctgcgc cgattgaaga acaaggttta
     1021 ggttgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc tggtctggaa
     1081 gtggtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa cctgttcaag
     1141 tatgagtggg tacagacccg cagtccggct ggcgcaatcc agttcgaagc ggtagacgca
     1201 ccggaaatta tcccggatcc gttcgatccg tcgaagaaac gtaaaccgac gatgctggtg
     1261 accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg tttcctcaac
     1321 gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac gcacagggat
     1381 atggggccga aatctcgtta catcgggccg gaagtaccga aagaagatct gatctggcaa
     1441 gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga tctgaaattc
     1501 gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg ggcttctgct
     1561 tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct ggcattaatg
     1621 ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt tctggagaaa
     1681 attcagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc aggtgtggtt
     1741 ggcgttgaga aagccgcaag cgccgctggt ttgagcattc atgtaccgtt tgcgccgggt
     1801 cgcgttgatg cgcgtcagga tcagactgac attgagatgt tcgaactgct ggagccaatt
     1861 gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt ccaccaccga gtcactgctg
     1921 attgataaag cccagcaact gaccctgacc gcgccggaaa tgaccgcgct ggtgggcggg
     1981 atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac tgaccgcgtt
     2041 ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga gtggaaagcg
     2101 accgacgaat cgaaagagct gttcgaaggc cgtgaccgcg aaaccggcga agtgaaatac
     2161 actgccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc ggtggctgaa
     2221 gtttacgcca gcagcgatgc tcacgagaag tttgttaaag acttcgtggc ggcatgggtg
     2281 aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca gcgactgcct
     2341 ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt ccacaggaaa actacacact
     2401 ggatctctca tgtctgccgc aggaaagagc aacccactgg caatcagtgg cctggttgtg
     2461 ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag ctacatcggt
     2521 gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt catcgtcctt
     2581 ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc cattgccctg
     2641 ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg cggagcgggg
     2701 aaggtggcga tcctgagcta taccatgccg ttctgggtgg tgattttcgc cgcggtgttt
     2761 ctcggtgaac gcctgcgacg tgggcaatat ttcgcgattc tgattgccgc tttcggctta
     2821 tttttggtgt tgcagccgtg gcaactcgat ttctcttcga tgaaaagtgc catgctggca
     2881 atcctctccg gtgttagttg gggggcgagc gcgattgttg ctaaacgtct gtatgcccgt
     2941 catccgcgcg tggatttatt gtcgttaaca tcctggcaga tgctctatgc ggcgctggtg
     3001 atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc cactgtgttc
     3061 tgggctctgg cctacagtgc gattctggcg acggcgttgg cgtggagctt atggctgttt
     3121 gtgctgaaaa acttgcctgc cagtattgcc agcttaagca cactggccgt tcccgtttgc
     3181 ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga aggtagcggt
     3241 attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga agccgttagt
     3301 gtaaaaagga tctgaatttt ttcttcatgt ggggcgatcg cttatttaac aaaataacga
     3361 taatgcccca ccatgcgcca gctaaacagc acatcttctt cctgcgcacc tgcgccaatg
     3421 ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgaaaccac cccaatatgt
     3481 gccagcccgt tatctaatcg ccaggagaca atatcgcccg cttgatagtc actggggttc
     3541 ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt aggcacccga
     3601 cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta ctcggcaaaa
     3661 ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct gcgcaatgca
     3721 cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc aggataggta
     3781 agctgcacat acgccggatc gtaaaatagc gtgctgccga tttgctgtct ggcaccgtct
     3841 gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaat ggcgggagat
     3901 tttaaggagt agctggtaaa tgccgttagc aggctgacca gcgccagtga agctttcatc
     3961 tgtttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg attgttctgt
     4021 agagaaaatg tccagaagcg gataaaaaat tcctgaacaa cgtgggagtt tcagattact
     4081 ggcacagagt gccggatgcg gcgtgaacgt cttatctggc ctaaaaagca cgcaaattca
     4141 atatattgca gagattgcgt aggcctgata agcgtagcgc atcaggcaat tttgcgttca
     4201 aactcccgga caagccggga gtttggagta ggttattccc actcttgcag gaaacgctga
     4261 ccgtactggt cggctaccag cagagcggcg taaacctgat ctggcgtcgc gccgccaggc
     4321 atgttgtgga tggtttcacc ttctgcacat gccgcttctg ccacaattcg cattttcgcc
     4381 gggacatctt ctttaatatc cagttgagcg agagttattg gcaaacctac cgcatggcta
     4441 agcgcagcta cggtttcgat ttcctcaacc ggcgagtttt ccagtaccag ctgcgtcagt
     4501 gtaccaaatg ccactttttc accgtgataa tagtgatggg catcaggaat ggcagtcagg
     4561 ccattatgca ccgcgtgcgc tgccgccaga ccaccgcttt caaagccaac accgcttaaa
     4621 taagtgttcg cttcaatcac gcgttccagt gccggagtca ctacatgctg ttcggcggcg
     4681 agcatcgctt tttcgccttc ttccagcaag gtgttgtagc acagttcagc cagcgccagc
     4741 gccgcctgag tgcacttgcc gcccgccatg gtggtcgcac cgctacgaga gcaggcacgc
     4801 gcttcaaacc aggtcgccag cgcatcgccg atacccgccg ctaacagacg cgcaggtgcg
     4861 cccgcgacga ttttagtatc gacaatcacc atattcgggt tatttggcag cagcagatag
     4921 cggtcaaatt caccttcgtc agtgtagata acggaaagag cgctacaagg tgcgtcggtt
     4981 gaagcaatgg tcggggcaat ggcgaccgga acacccatga aatgtgccag tgctttggct
     5041 gtatcgaggg tttttccgcc accgataccg agaattgcgc cacactgcgc agtctccgcg
     5101 atgccacgca gacggtcgat ctcattttgc gaacattcac cgccaaacgg cgcaatctct
     5161 actaccagtc cagcatcttt aaagcttttc tcgacagtgg attgagcaaa acctaaaaca
     5221 aatttgtcac ccaccactaa ccagcgttct gccagcggct tcaggtattc gcccagacga
     5281 ttaatcacat cagcgccctg gatgtattta cccggtgatt gaataatgcg gtccataatt
     5341 gctcctttag agatgagtag tgccaaatgc ggcattccag tcgtgttcga acttctctat
     5401 agctgactct accgcagggg tgttgagcat ttgttgcgct acatctaagg gcagggtgat
     5461 ggattcacat cctgccagta aacagtccag cgcctgacgc ggcgttttaa agctggccgc
     5521 cagcaccatg ctttctggtg cgtgcatttc taacagcgct tgcagctcct gaaccgtacg
     5581 aatgccgtcc ccgccctggg catccacgcg gttaacatac ggagcaacgt attttgcccc
     5641 tgccagtgcg gctaataacc cttgtgcggc gctatataca gcagtgccaa gtgtagtaat
     5701 accctctttt ttcagtattt taattgctgc cagaccttcg gaagtcaccg ggattttcac
     5761 cacaatgccc ggaatggcat cgcgcagacg cttcgcttct tccaccatcc cctgcgcgtc
     5821 gcggctcatg gtctgagcaa acagaatgcc ctcatcacca atcgcttttt gcagacgcgg
     5881 cagcacttcc catatggact ccttgctggc agcgataatg ctcgggttag ttgtcacccc
     5941 ggcaatcggg aatatgtgtg ccagacgttc gacttctgcg acgttagcgg tgtccagata
     6001 cagttccatg atgtttcctt ttacagttcc agttcatgtt gcagcaggct ggcgatagcg
     6061 tcggcagagg cggcgttaac cagcgcgtta cggaattctt cgtgcatgat gcggcgagcg
     6121 aggcgcgaga aaatgcgcat atgctgatcg cccgcagcgt gtttgttcag ggttaacata
     6181 atgatgaatt gcgcttcatc atcgccccag cgcaccggcg cttgcagacg cgctacgctg
     6241 atggtggatt gctcaatgtg ttctgatttg ctgtggggaa tggcaaaact aaagcccaga
     6301 ccggtagaga aaacggcctc gcgcgcccac aagtcggctt ccagtttacg cggatagcgg
     6361 cagcggcccg ccagcagcag gttatcggtc atgcctttga gcacttcttc tttgctgcgc
     6421 cagtcgcttt ccagtgtgat gcactcggcg gtgaccagcg gtgcgtcttg ttgggtcatg
     6481 cggaattgcg ccagcaggtg ttccacttcc agcgaagtac ggcaggccat tgcctggttg
     6541 agcaacttgc ggcactcacg gctatcaagt tgcgccatcc gcgctttcgc cgccggaatt
     6601 gatggtgcgc tcatgctgag ttcatccagc cctaagccga ccagcaacgg cagcacggaa
     6661 cctttcgctc ccagctcacc gcacagacca atccatttgc cctggcgatg caccgcttgc
     6721 acggcgtaat cgagcgcccg caagaatgcc ggattcaggc tgttgtagtg acgagtaacc
     6781 ttagcgttat cgcgatccac tgccagcaga tattgcgtca ggtcattact accaatacta
     6841 aagaaatcaa tctcttcgca gcattgatcg atgatgaaca tcaccgacgg cacttccagc
     6901 atgatgccga gctggatctt ctcatcaaac ggaatgtgtt cgttacgcag ttgctgtttg
     6961 gcttccgcca gtttttcttt cacccataag atctcttcca ttgaggagat catcgggatc
     7021 atgattttca ggctgccgtg agcagaggca cggaggatcg accgtagttg tgtggtgaac
     7081 agcgaggcgt attcttcata aatacgcacc gcgcgataac cgaggaacgg gttagcttct
     7141 gccggaatgt tcagataatc aacgggtttg tcaccgccaa tatccatagt gcgcacaata
     7201 atgctgcgtc cgttggcgga ctccagcgct tgacaaaaaa tgttgtacaa ctcgctttcg
     7261 cccggtgcgc tggtgcgatc catatagagc atttcagtgc ggaacaaacc gacgccttcc
     7321 gcaccattgc cgaatgcggc ctgcgcttcc acggagtgag cgatgttagc ggcaatttca
     7381 atgcggatac cgtcagcggt acgggcttgt tgggtcagcc agacacgctg ttgctcacgc
     7441 agggcgtcct gtacgcgggc ttcttgctga taataacgag ctacggcttc ccctggctca
     7501 accacaatcg ccccggcgtt gccgtcgata taaatcgttt gatgctgcca cggagtaagg
     7561 gcatcaatat ctacaccaac cagtgtcgga atgttgaacg aacgggcaag aattacggta
     7621 tgtgaggtgg tgccgccact tttcagtaac agacctttga ggtgattttt atccagttcg
     7681 aggaactggc tgggggttag ttcatcagcc atacaaatgg cgggctgcgt cagtttgccc
     7741 ggtgccggga agcgttgctc accgtagatt tgctgaagca gctggaagca gacatcgcgt
     7801 acgtccaggg cacgttcttg caggtagctg ctgctggaac gggaaaactc ttcacaaaag
     7861 tgattcgcac tggcgacaat cgcttcggcg cagcttaatc cggcgctgac gcctgccagt
     7921 aaatgttcgt gcagggaagt atcgccagcc agggatcggt gcgcttccag aatcgcgctg
     7981 gtggcaccgt cgctatccag cagacgaaac tcaatgtttt tcagtaccag cgtcaggccg
     8041 ttttccagtg cggattgctc ggcgtcaacg tctttggctg cgggaagatt acccagcgca
     8101 ttgagatcta aagaagagat cggcgtcaga atgccgcctg cgctaccgct gcacacggtg
     8161 cgggcgcgga taatttgcgg attcagattg gtcagtgaaa ccggcagtgg ttccagttcg
     8221 tcagatttaa cttccgccag cggcgcatcg cagtggggga attcatcgcg cagccattgg
     8281 cttaaacgct ggtgcgcttc ctgttcgtcg gccccggaaa tcagtaactg gcagttatcg
     8341 cccgccagcg tgtcggtgcc aatcagcgca agggcgcttt tggcgttgcc cttgcggtca
     8401 gtgcgcaggt tatgccactc aatttgtgat gaaaaagtat tacacagcgt ttcaacgtgg
     8461 cttgccggac gtgcatgtac gccgttaggt agctcacaaa taaattccac aatcagggcc
     8521 attgcctctc tcccggtacg gttttctgtt tacaggataa gagcgcacgg caacggcctg
     8581 ccatgtgaca aatctgccaa aagctggaca aatgtaatgt aaccgtcaat ttgcgacgcg
     8641 tctcacaaga cgccgttttg cggcatgctt ccggattatc gcaagttatg aggcggatcg
     8701 catttttgta ctgatattac aaaagtccag taaatggcct ttttatccac tgttttctcc
     8761 gcctgcgatt gcctattgtt tcctccatca aaaatatgga catgggcttt cgcaagcccg
     8821 ggagcagctt atgaatgagt tggtgcagat cctgaaaaat acccgtcagc atttaatgac
     8881 gggcgtttca cacatgattc ccttcgtggt atcgggcggt attttgctgg cggtttccgt
     8941 catgttgtat ggcaaaggcg cagtgccgga tgccgtagcc gatccgaatc tgaaaaaact
     9001 gtttgatatc ggcgttgcag gtttgacgct gatggtgcct ttcctcgccg cttacatcgg
     9061 ttactccatt gcagagcgtt ctgcgctggc tccgtgcgct atcggtgcct gggttggtaa
     9121 cagctttggt gcgggcttct ttggtgcact gatcgccggg attatcggcg gcatcgtggt
     9181 gcattacctg aagaaaattc cggtgcataa agttctgcgc tcggtgatgc ccatcttcat
     9241 cattccgatc gtcggcacac tgattaccgc aggcatcatg atgtggggtc tgggcgagcc
     9301 tgtaggggcg ttgaccaaca gcctgactca gtggcttcag gggatgcagc agggcagcat
     9361 tgttatgctg gcggtgatca tgggtctgat gctggcgttc gatatgggcg gtccggttaa
     9421 caaagtggcc tatgccttca tgctgatttg cgttgctcag ggtgtttata ccgtggtggc
     9481 cattgctgcc gtgggtattt gtatcccgcc gctggggatg gggctggcga cgctgattgg
     9541 tcgtaaaaat ttctccgcag aagagcgcga aaccggtaaa gcggcgctgg tgatggggtg
     9601 cgttggggtt actgaagggg cgattccttt cgccgcagcc gatccgttac gtgtaattcc
     9661 ttccatcatg gttggttcag tttgtggtgc agtaactgcg gcgctggtcg gtgcgcagtg
     9721 ctatgcaggc tggggtggtc tgattgtgct gccggtggtt gaaggtaagt tgggttatat
     9781 cgcagcagtg gctgtcgggg cggttgtgac ggcagtctgc gtgaacgtgc tgaaaagtct
     9841 ggcgcgtaaa aatggatctt ctactgatga aaaagaagac gacctggatt tggattttga
     9901 aattaattaa ttgaggaacg agccatgacg aaaattattg cagtaaccgc gtgtccatct
     9961 ggtgttgccc atacttatat ggctgcagag gcgctggaaa gtgccgcgaa agcgaaaggc
    10021 tgggaagtga aagtagaaac ccagggctcg attggtctgg aaaacgaact gactgcggaa
    10081 gacgtggcga gcgccgatat ggtgattctg actaaagata tcggcatcaa gtttgaagag
    10141 cgttttgctg gcaagaccat cgtgcgcgtc aacatcagcg atgcggtaaa acgtgctgat
    10201 gccattatga gcaaaattga agctcacctg gcgcaaaccg cttaagttct tctcccgctc
    10261 gcaagggcgg gttcgctttc ccacaggagt tcctcatgac gaatcgtatc tctcgcctca
    10321 aaactgcact gtttgccaat acccgtgaaa tctcgctgga gcgtgcgctg ctttataccg
    10381 ccagccatcg gcaaaccgaa ggcgaaccgg tgatattgcg ccgggcgaaa gcaacagcgt
    10441 atatccttga acatgttgaa atttcgattc gtgatgaaga actgattgcc ggtaaccgca
    10501 ccgtaaaacc gcgcgccggg attatgtcgc cggaaatgga cccttactgg ctgctgaaag
    10561 agctgggtca attcccgacg cgtccgcagg accgttttgc tatcagcgaa gaagataaac
    10621 gtatctaccg cgaagagttg ttcccgtact gggaaaaacg ttcgatgaaa gatttcatca
    10681 acgggcagat gacggatgaa gtaaaagccg cgactagcac gcagattttc agcatcaacc
    10741 agacggataa aggccagggg cacattatta ttgattaccc gcgcctgctg aatcacgggc
    10801 tgggggagct ggtggcacag atgcagcaac attgtcagca acagccggag aatcactttt
    10861 atcaggcagc gctgttactg ctcgaagcct cgcagaaaca cattttgcgt tacgccgaac
    10921 tggcggaaac gatggcggca agctgcactg atgcccagcg tcgcgaagaa ctgttgacta
    10981 ttgcggagat ctcccgccat aacgccgaac ataaaccgca gacgttctgg caggcgtgcc
    11041 agttattctg gtacatgaac atcattctgc aatacgaatc caacgccagt tcgctgtctt
    11101 tagggcgctt cgaccagtat atgttgccgt tctatcaggc atctttaacc cagggcgaag
    11161 atccggcgtt cctgaaagaa ctgctcgaat ctttatgggt gaaatgcaac gacattgtgc
    11221 tgttgcgctc caccagcagc gcgcgttatt tcgcaggttt cccgactggc tataccgcac
    11281 tgctcggcgg gttaaccgag aacggacgta gcgcggtgaa cgtgctttcg ttcctctgcc
    11341 tcgatgccta tcaaagcgta caattaccgc aaccgaacct aggggtgcgc actaacgcct
    11401 tgatcgacac gccgttcctg atgaaaaccg cagaaaccat tcgcctcggc accggtattc
    11461 cgcaaatctt taacgatgaa gtggtggtgc cagcgttcct caaccgtggc gtttcgctgg
    11521 aagatgcgcg cgactattcc gtagtgggct gtgtggaatt atctattccc ggcagaacct
    11581 acggcttgca tgacatcgcg atgtttaatc tgctgaaagt gatggaaatc tgcctgcatg
    11641 aaaatgaagg caatgctgcg ctgacttatg aaggtttact ggagcagatc cgcgccaaga
    11701 tcagccacta catcaccctg atggttgagg gcagcaatat ttgtgatatc ggccatcgcg
    11761 actgggcacc tgtaccgctg ctctcgtctt ttatcagcga ttgtctggaa aaaggtcgcg
    11821 atattaccga tggcggcgcg cgttataact tctccggcgt acagggaatt ggtatcgcca
    11881 acctgagcga ttctctccat gcgttgaaag ggatggtttt tgatcaacag cgtttaagtt
    11941 ttgacgaatt gctgtcggta ttaaaagcca actttgcaac gccagaaggc gaaaaagtcc
    12001 gcgctcgctt aattaaccgc tttgagaaat acggtaacga tatcgacgag gtgggtaaca
    12061 ttagcgccga actgttgcgc cactactgca aagaagtgga aaaataccag aacccgcgcg
    12121 gcggttactt cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg
    12181 ttggcgcgac gccagacggt cgttttgccg gagaacagtt ggcagacggc ggcttgtcac
    12241 ccatgctggg ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg
    12301 ataacacgct gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg
    12361 aaggtgaagc gggattacgc aaactggccg acttcttacg ggcgtttacc cagcttaagt
    12421 tgcagcatat tcagtttaac gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc
    12481 cacaagatta tgccgggctg gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac
    12541 tgtcgaagga gatccaggat gacatcatcc gccggacagc gcatcagctg taacgttgtg
    12601 gaaacgcgcc gcaatgatgt ggcgcgcatt ttcaacattc agcgttattc actgaatgac
    12661 ggcgagggca ttcgtacggt ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt
    12721 gctaatccgg agtcgatctc cggcaaaatc cagacggtac gcagagaggc gaaatgtctg
    12781 cactgtgcga aatgtttgcg tgatgcggat gaatgcccct ccggggcgtt tgaacggatt
    12841 ggtcgcgata tcagccttga cgcgctggaa cgggaagtga tgaaagatga cattttcttt
    12901 cgcacgtccg gcggcggcgt cacgctttct ggcggcgaag tgttaatgca ggcggagttt
    12961 gctacccgtt ttttacagcg gctgcggttg tggggcgtgt catgcgccat tgaaactgcc
    13021 ggagacgcgc cagccagcaa gctgttaccg ctggcgaaat tgtgcgatga agtgttgttc
    13081 gatttaaaaa ttatggacgc gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc
    13141 gtgctggaga atctgcgttt gctggtgagt gagggcgtca acgtgatccc gcgtttaccg
    13201 ctgatccctg gtttcacgct cagccgggag aatatgcagc aggcgctgga tgtactgatc
    13261 ccgctgaaaa tcaggcagat ccatctgtta ccgtttcatc agtacggcga accgaaatac
    13321 cgcctgctgg ggaaaacatg gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg
    13381 acaacgatgc gcgaaatggc agaacgggcc ggatttcagg ttaccgtggg aggttaaaat
    13441 ggcatacctg gtggcagtaa ccgcctgcgt aagcggcgtg gcgcatacat atatggcggc
    13501 ggaacggctg gaaaagttgt gcctgttaga gaagtgggga gtcagcattg aaactcaggg
    13561 cgcgctggga acggagaatc gtttagcgga cgaggatatc cgtcgggcgg atgttgctct
    13621 gttgattacg gatatcgagc ttgccggtgc cgagcgattt gaacattgcc gctatgtgca
    13681 atgcagcatc tacgcattcc tgcgtgagcc gcagcaggta atgagcgcgg tgcgcaaagt
    13741 gctttctgcg ccgcagcaaa cccatcttat tctggagtag tcggtttttc tgtcagttgg
    13801 ctgtggtact gccggcgata ttccgacggc gagcgttcgg tgtttttacg aaacagacgg
    13861 cagaagtagt tgctgtcgac aaagccgcag gcgtgcgcca cttcttttac cttcaggtca
    13921 tagcctttca ataacgtctt agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa
    13981 ccaatggccc ccgttttttg aaacaggtgc gagagatagt ttggtgaaat atagaatgcc
    14041 tgtgcgacag attcgcgggt aagcgcggag gcatagcgtt cgtcgatata atcgcgaata
    14101 gcttcgaata gtgcctggct gcgtgaggcg gtctggattt ggctgccaag caaatcgcgg
    14161 cagtggctaa gcaggctggc gacgataagc cttgccgttt gctgctcctg cggctgcatt
    14221 tgcatttcat tgagcgtttg tagcagaaaa gaacctatgc gtgggccgcg tcgggcgacg
    14281 tgttgcttcg ccagattttg atattgtttg ccatcccatt gcacgacgct gaagccgagt
    14341 tgctgtttgc caaacagcac gctaaaggta gtagcagggg cttgccattg tggaaaattc
    14401 cagccaccag cggcaacgta cagcacatcg ccgggaacta acgttgctcc agcgccggta
    14461 tcaacaaact caccttccag cacaatttcc agccgtggaa aatcgacctg atacgcaaga
    14521 tcgggcaccg ggccgttact gctggcaaag taaatctggc gcagggacag cgggccgttg
    14581 atcaggcggg agagcagata gctgacgtcg tgatacatgt tattccctta ggtatggaga
    14641 cgggaaaaca gtaactggca ttattaaaaa aggccacctg aaaagtgtgg cctgagggga
    14701 gttcgatgta aacattgcgg cagttgtatt gccggatgtg gcgtaaacgc cttatccagt
    14761 ctaccgccgc gatctgtagg ccggataaga cgcgtcaagc gtcgcatccg gcaaatagcg
    14821 cctggctgat aattactgat tacctacctg gtcgccatac ggcagcgtgt cgtaatcgat
    14881 cagcgcgttt ttcttgtacg ggttaccaat ccagcgggta ttttctttga actgcggatt
    14941 caccactgaa cgggttggat cgtaaccgtc gtaagataag cccgctaaat ctgaccaggt
    15001 gtggatcagt tccgcaaggc tgtatttacg atcaacatcc tgcgagaaat cacgcggatg
    15061 agtcgcttgc catttttccg acgtccacag caggaacgga atggtgtaca tatgacgcgt
    15121 cgggttgtcc tcattacgcc cctgggtttt atgaggcggc gtgtcgtaaa cctcttcacc
    15181 gtggtcagag aagtaaacca ggaaaccgtt cgggtctgct gctttaaagt ctttaatcag
    15241 gctggcaacc acatgatcgt tatacaggtt agcgttgtca taatcgttat atgactccag
    15301 ctcttccgcg tttaatcccg gcggaacatg atcggtattg ccatcaaact tgccctgatt
    15361 ttccgggtag cggtatttgt atttgatatg cgtacccagc agatgaacga tgatcagttt
    15421 cttcggcgca gggtccttca gcacttcctg gaacggcttc agcacgttag tgtcgtattc
    15481 acgcgcactc tgcgtacgtt gctggttcat gtagtactgc ttgtcggtct ggcgcgaaaa
    15541 taccgtcagc atggtattgc gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt
    15601 ataacccgcc tgtttcatca tgttcatcag cgacggctgc gtcagataca gatccgggtt
    15661 cttttcattg gcaaaggtca gcgcctgttg caggatttca atggtgtacg gacgagacgt
    15721 aactacgtta ttaaacacgg tcagattcgg atcggtttta tgcagcgcat tcagctccgg
    15781 cgtggtttca cgcggataac cgtacagact catgcgtcca cgctgggttg actcgccaat
    15841 caccagcact aaggtgcgcg gttcgttacc cgattcatct ttgaaattag ccagcggcgg
    15901 caaggcatta ttttcattca gtagctttgt tagcgagttt agttgctgac gatactgata
    15961 atagccggtc aggaattgcc acggtgcggc aggctccata cgcgaggcca ggttatccaa
    16021 cgttttctca aacggcttgt ttttgataaa catattcatg gcgatcggat gcagaatcaa
    16081 gccataaagc agggcaaaag agacaacata acgccacggc tttggaatat agaccgggcg
    16141 caggcgtgtc cacagcagaa ctgccaccgc cgtataggcc agcgcgataa gcacaatttt
    16201 caggctgaaa tactggctta aatactcgct ggcttcgttg gtgttggttt cgaacatcac
    16261 aaacagaacg ctctgcgaga actcctgacc gtagatgacg tagtagcaca gcgccgccag
    16321 agaggccgcc catagcacca cgccgatcac tgcggcaata attttgatcc gcttcggaaa
    16381 gaggaatacc ggaattaacc acagcgagct gaataacagc gagtcgcgaa tgccgttggt
    16441 gccactataa ccactgatgt aaataatggc ctgtagcaga gtagagaaaa accaaaagta
    16501 gagcaatgcc caacccaggg ctttccagct aaaaagaggt ttagcctgga cttctgtgga
    16561 atgcatagta agaacctgtc ttgaaaaaaa tatcgccgaa tttaccgaca attccttaag
    16621 gatatctgaa gggatatcca gaatttgaat aaaatgcaga cagaaatata ttgaaaacga
    16681 gggtgttaga acagaagtat ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagga
    16741 gaggaagatt agccggtatt acgcatacct gccgcaatcc cggcaatagt gaccattaac
    16801 gcctgctcga cgcgaggatc cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc
    16861 agcaactcgg cttgcaatac gttcagcggg tcggtgtaaa tattccgtag ctgaatagac
    16921 tctgcaatcc acggcagatc ggccatcaga tgggaatcgt tggcaatcgc cagcaccact
    16981 ttgatgtctt cttcttgcag attgcgtaac tctttaccta acggccacag tgttttgtct
    17041 accaggcgtt ggtcatagta ttccgccagc cacaggtctg ctttggcgaa gaccatctcc
    17101 agcatgccga gacgcgtcga gaagaatggc caatcgcggc acatagcctc cagctcgctc
    17161 tgtttgccat cttcgaccac tttttgcagc gccgtacctg cacccagcca ggcggggagc
    17221 atcagacggt tttgcgtcca ggcgaagatc cacggaatgg cgcgtagtga ctcgacgccg
    17281 ccggttgggc gacgtttcgc cggacgtgaa cccaacggca gtttgcccag ttcctgttcc
    17341 ggcgtagcgg agcggaagta aggcacaaaa tctttgtttt cacgtacgta gccacggtag
    17401 agatcgcagg agatgactga tagttcatcc ataatgcgac gccagctctc tttcggctca
    17461 ggtggtggca gcaggttggc ttccagtatc gccccggtgt aaagcgacag gctgctgacg
    17521 gtgatttctg gcagaccata tttaaagcgg atcatctcgc cctgttcagt cacgcgcagg
    17581 ccacctttca ggcttcctgg cggttgtgac agcagcgccg catgagcagg tgcgccgccg
    17641 cgaccaatgg aaccgccgcg accgtggaac aacgtcagct caatacccgc tttttcgcag
    17701 gttttgatta atgcatcctg tgcctgatat tgcgcccagg acgctgccat cacgcccgca
    17761 tcttttgctg agtcggaata gccaatcatc accatctgtt tgccctgaat caggccgcga
    17821 taccagtcga tattcagcaa ctgagtcatg acatcgttgg cgttgttcag gtcatcgaga
    17881 gtttcaaaca gcggagcaac cggcatcgca aacccgatac ccgcttcttt cagcagcagg
    17941 tggacagcca gcacgtcgga cggcgttttc gccatcgaga tcacgtaggc ggcaatggag
    18001 ccttgcggtg cttcggcaat cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt
    18061 ggttgccagt tgcgcggcag aagcggacgt ttggagttca gttcgcggat caggaacgcc
    18121 tgtttgtcgg cctctgacca gctttcgtag tcgccgatac cgaggtagcg ggtcagctcg
    18181 cccagcgctt cggtatgacg cgtgctctcc tgacggatat caatacggac cagcggtacg
    18241 ccgaaacatt tcacgcggcg cagggtgtcg agcagatcgc cgttggcgat aatacccatg
    18301 ccacacgcct gaagtgactg gtagcaagcg tagagcggtt cccacagttc ttcgttttgt
    18361 gtcagcaggc cttctggttt tggcagttct tcgcctttca ggcgcgcttc cagccatgcc
    18421 tgtgtcgcca tcaggcgaga acgcaggttt ttcatcagat agcgatacgg ttctgcggca
    18481 ccttcttcgc caaccagcgc cagcagttca ggggtcgctt caaccatcga cagttcagaa
    18541 accagaacct gaatatcttt caggaacaga tcggtggctt tccagcggct gagcagcagg
    18601 acgtggcggg tgatatcggc agtgacgttc gggttgccgt cgcggtcacc gcccatccac
    18661 gaggtaaaac gtaccggaac aaattcgacg ggcagtttgt agccgaggtt ctcttccagt
    18721 tgttcgttca gttcgcgcag gtaattcggt acgccttgcc acaggctgtt ttctactacg
    18781 gcaaagcccc atttggcttc atctaccggg cttggacgca gcttacggat ttcatcggta
    18841 tgccatgact gggcgatcaa ctggcgcagg cgacgcatca gctggttgtg ttcgtagtca
    18901 gcgatatctt tgttatcgag ctgttttaag caggcgttca cttccaccat tttgtggatc
    18961 agtgtacgac gggtaatttc ggttgggtga gccgtgagga ccagttccag cgacagcgat
    19021 tccactgctt ttttgatggt gtcttcgctc agttccggct ggtttttcag tttacgcagg
    19081 gtgcgggcga tcacttccgg gttgctggca gcttcgcctt tcggcgaaat gctgtggtat
    19141 tgctcggcgg tgttggccag gttcaggaac tgactaaacg cacgcgcaac gggcagcagc
    19201 tcgtcgttcg acaaattttg taaggtggtg agtaactcct ggcggttagc atcattgcca
    19261 gcgcgtgaag atttggacaa cttacggata gtttctacgc gttcaagaat gtgttctccc
    19321 aacgcatcct tgatggtttc tcccagcact ttgccgagca tactgacatt actacgcaat
    19381 gcggaatatt gttcgttcat attaccccag acaccccatc ttatcgtttg atagccctgt
    19441 atccttcacg tcgcattggc gcgaatatgc tcgggctttg cttttcgtcg tcttttataa
    19501 agccacgtaa aagcggtgac gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag
    19561 caggaattta cgtcattaaa ttcacgacgc tttaaataag cgtaacttat gggaatgtta
    19621 aaaaatcgcc tcaagtaaca ccaaaggtgt aggtcggata agatgtgcaa gcatcgcatc
    19681 cgacattatt gcggcactgg agtttggcaa cagtgccgga tgcggcgcaa gcgccttatt
    19741 cggcctacgt tttaatgcca gcaaaaatgg tgaatgacct gggttatcag ttcgcgggtg
    19801 ggcttgataa accgtgtttc cagatattca tcaggctgat gagcctgatt aattgagcca
    19861 ggccccagaa ccagcgtcgg gcataacgtt tgaataaacg gcgcttcggt acagtaattc
    19921 accacttcgg tttttgctcc gagcaatttc tcaaccactt caaccagttg atgattcggt
    19981 gggcattcat agccagggat cggcggatgc agctcgtcga ccgtcagacg acccggccag
    20041 cgttcgctca ccggagccaa tgcatcgttg agcaaaccat taagttcatt gagtgtcatg
    20101 ccaggcagcg gacgaatatc catatgcaac tcacaccaag cgcaaatacg gttagaagcg
    20161 tcgccaccgt gaatatgccc gaggttgagc gtagggtatg gcacggtaaa cgcttcgtag
    20221 tgataacgtt ctttcaggtt atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatt
    20281 agttctatag cgttaactcc gcgtgctgga tcgctggagt gccccgactg gccctgaata
    20341 cggatggcgt tagagatatg acctttatgt gcgcgtaccg gttgtagtga cgtcggttcg
    20401 ccaatgatgg cgcaatccgg gcgcagggcg gtagtttcgg caaaataacg cgctccggcc
    20461 atactggttt cttcatcagc agtcgccaga atgtagagcg gttttttcag tttcgtgacg
    20521 tcgacatcgc gtagcgcatc aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct
    20581 aagccgtaaa gcttgccgtc atgctccgtc agtgtaaacg gatcgcgcgt ccagcgaccg
    20641 tcatcaaatg gcaccgtatc ggtatgcccc gccagcaaca agccgccagc cccctgtccg
    20701 gtacttgcca gcatattaaa tttgttgcga gttcctggta caggctgcac ttccacattg
    20761 aagcctaaat ctttaaacca gtccgccagc agagtgatta aatctgcatt gctttgatcg
    20821 agtgcctctt ccgtggcgct tattgaaggt gtggcaatca gagcacggta aatctcgata
    20881 aatggcggta atttgttttt cattgttgac acacctctgg tcatgatagt atcaatattc
    20941 atgcagtatt tatgaataaa aatacactaa cgttgagcgt aataaaaccc accagccgta
    21001 aggtgaatgt tttacgttta acctggcaac cagacataag aaggtgaata gccccgatgt
    21061 tgaatacgct gattgtgggt gccagcggct acgctggcgc agagctagtg acctgtgtaa
    21121 atcgccatcc gcatatgaac ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg
    21181 gaaagttaat ctccgatttg catccgcagc taaaaggcat cgttgatctg ccgttgcagc
    21241 cgatgtcgga tatcagcgag tttagccctg gggtggacgt ggtgtttctc gccaccgccc
    21301 atgaagttag ccacgattta gcgccgcagt ttcttgacgc gggctgcgtg gtgttcgacc
    21361 tttccggcgc gtttcgtgtt aacgacgcca ccttctatga aaaatattac ggctttaccc
    21421 atcaataccc ggaactgctg gaacaggcag cctacggtct ggcggagtgg tgcggtaata
    21481 aattaaaaga agcgaatttg attgcggtgc caggctgtta tccgacggca gcacagttgg
    21541 cgctgaaacc gttgattgat gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg
    21601 ccaccagcgg cgtgagcggt gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag
    21661 ttagcctgca accgtatggc gtctttaccc atcgccatca accagagatc gccacacacc
    21721 tcggtgctga cgttatcttc accccgcatc tgggcaattt cccgcgcggc attctcgaaa
    21781 ccattacctg ccgcctgaaa ccgggtgtga cccaggcgca agtggcgcaa gcgttacagc
    21841 aggcgtatgc ccataaaccg ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa
    21901 atgtcgttgg gctaccattt tgcgatatcg ggtttgccgt tcagggcgaa catctgatta
    21961 ttgtggcgac cgaagacaac ttactgaaag gcgcggcggc acaagcggta cagtgcgcca
    22021 atattcgttt cggctatgcg gaaacgcagt ctcttattta agggtgcaat gatgaatcca
    22081 ttaattatca aactgggtgg cgtactgctg gatagtgaag aggcgctgga acgtctgttt
    22141 agcgcactgg tgaattatcg tgagtcacat cagcgtccgc tggtgattgt gcacggcggc
    22201 ggttgcgtgg tggatgagct gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaatggc
    22261 ctgcgggtga cgcctgctga tcagattgac attatcaccg gagcactggc gggaacggca
    22321 aataaaaccc tgttggcatg ggcgaagaaa catcagattg cggccgtagg tttgtttctc
    22381 ggtgacggcg acagcgtcaa agtgacccag cttgatgaag agttaggtca tgttggactg
    22441 gcgcagccag gttcgcctaa gcttatcaac tccttgctgg agaacggtta tctgccggtg
    22501 gtcagctcca ttggcgtaac agacgaaggg caactgatga acgtcaatgc cgaccaggcg
    22561 gcaacggcgc tggcggcaac gctgggcgcg gatctgattt tgctctccga cgtcagtggc
    22621 attctcgacg gcaaagggca acgcattgcc gaaatgaccg ccgcgaaagc agaacaactg
    22681 attgagcagg gcattattac tgacggcatg atagtgaaag tgaacgcggc gctggatgct
    22741 gcgcgcacgt tgggccgtcc ggtagatatc gcctcctggc gtcatgcgga gcagcttccg
    22801 gcactgttta acggtatgcc gatgggtacg cggattttag cttaagtttt gttggccgga
    22861 ggcgcagctt tccggcattg aatttcaaaa taaggaaaca gagttatggc actttggggc
    22921 gggcgtttta cccaggcagc agatcaacgg ttcaaacaat tcaacgactc actgcgcttt
    22981 gattaccgtc tggcggagca ggatattgtt ggctctgtgg cctggtccaa agccctggtc
    23041 acggtcggcg tgttaaccgc agaagagcag gcgcaactgg aagaggcgct gaacgtgctg
    23101 ctggaagatg ttcgcgccag gccacaacaa atccttgaaa gcgacgccga agatatccat
    23161 agctgggtgg aaggcaaact gatcgacaaa gtgggccagt taggcaaaaa gctgcatacc
    23221 gggcgtagtc gtaatgatca ggtagcgact gacctgaaac tgtggtgcaa agataccgtt
    23281 agtgagttac tgacggctaa ccggcagctg caatcggcgc tggtggaaac cgcacaaaac
    23341 aatcaggacg cggtcatgcc aggttacact cacctgcaac gcgcccagcc ggtgacgttc
    23401 gcgcactggt gcctggccta tgttgagatg ctggcgcgtg atgaaagccg tttgcaggat
    23461 gcgcttaagc gtctggatgt cagcccgtta ggttgtggcg cgctggcggg aacggcttat
    23521 gaaatcgacc gtgaacagtt agcgggctgg ctgggatttg cttcagcaac ccgtaacagt
    23581 ctcgacagcg tttctgaccg tgaccacgtg ctggaactgc tttctgctgc cgctatcggc
    23641 atggtgcatc tgtcgcgttt tgctgaagat ctgattttct ttaacaccgg cgaagcgggg
    23701 tttgtggagc tttctgaccg cgtgacttcc ggttcatcat taatgccgca gaagaaaaac
    23761 ccggatgcgc tggagctgat tcgcggtaaa tgcggtcggg tgcagggcgc gttaaccggc
    23821 atgatgatga cgctgaaagg tttgccgctg gcttacaaca aagatatgca ggaagacaaa
    23881 gaaggtctgt tcgacgcgct cgatacctgg ttggactgcc tgcatatggc ggcgctggtg
    23941 ctggacggca ttcaggtgaa acgcccgcgt tgccaggaag cggcgcaaca gggttacgcg
    24001 aacgccactg aactggcgga ttatctggtg gcgaaaggtg tgccgttccg cgaggcacac
    24061 catattgtgg gtgaagctgt ggtggaagcc attcgtcagg gcaaaccgct ggaggagctg
    24121 ccgctcaccg agttgcagaa atttagtccg gtgattggtg aagatgtcta tccgattctg
    24181 tcgctgcaat cgtgcctcga caagcgtgcg gcaaaaggcg gcgtctcacc acagcaggtg
    24241 gcgcaggcga ttgcttttgc aagggcgcgg ttggggtaag aacatttata tgtacaaatt
    24301 tgagcctggc ttatcgccgg gcttttttat ggcaaaaaaa agcggatcct ggagatccgc
    24361 aaaagttcac gttggcttta gttattcgag ttgagaaact ctcgaaacgg gcagtgactt
    24421 caagggttaa aagaggtgcc gctccgtttc tgtgagcaat tatcagtcag aatgcttgat
    24481 agggataatc gttcattgct attctaccta tcgccatgaa ctatcgtggc gatggaggat
    24541 ggataatgaa tattcgtgat cttgagtacc tggtggcatt ggctgaacac cgccattttc
    24601 ggcgtgcggc agattcctgc cacgttagcc agccgacgct tagcgggcaa attcgtaagc
    24661 tggaagatga gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc
    24721 aggcgggaat gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta
    24781 aagagatggc aagccagcag ggcgagacga tgtccggacc gctgcatatt ggtttgattc
    24841 ccacggttgg accgtacctg ctaccgcata ttatcccgat gctgcaccag acctttccaa
    24901 agctggaaat gtatctgcat gaagcacaga cccaccagtt actggcgcaa ctggacagcg
    24961 gcaaactcga ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc
    25021 cgttgtttga tgagccaatg ttgctggcta tctatgaaga ccacccgtgg gcgaaccgcg
    25081 aatgcgtacc gatggccgat ctggcagggg aaaaactgct gatgctggaa gatggtcact
    25141 gtttgcgcga tcaggcaatg ggtttttgct ttgaagccgg ggcggatgaa gatacacact
    25201 tccgcgcgac cagcctggag acgctgcgca acatggtggc ggcaggtagc gggatcactt
    25261 tactgccagc gctggctgtg ccgccggagc gcaaacgcga tggggttgtt tatctgccgt
    25321 gcattaagcc ggaaccacgc cgcactattg gcctggttta tcgtcctggc tcaccgctgc
    25381 gcagccgcta tgagcagctg gcagaggcca tccgcgcaag aatggatggc catttcgata
    25441 aagtgttaaa acaggcggtt taaaccgttt aacgcagcta cccgataggc ttccgccatc
    25501 gtcgggtagt taaaggtggt gttgacgaag tactcaatag tgttgccgcc acctttctgt
    25561 tccataatcg cctgaccgat atgaataatt tcggcagcgc gctcgccaaa gcagtgaatg
    25621 cccagaatct cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg
    25681 acgatttgtg cacgtgccag atgtttgaac tgggcgcggc ccacttcata tggcactttc
    25741 atcgcggtca gctgctgttc ggttttgccc acagagctga tttccgggat ggtgtaaatg
    25801 ccggtaggga tatcttcaat cagatgcgcg gtggcttcgc cttttaccag cgcctgcgcg
    25861 gcaatgcgcc cctggtcata ggccgctgat gccaggctcg gataaccaat cacgtcgccc
    25921 accgcgtaaa cgtgcggctg tgcggtctga tacatgctgt tgaccttcag ctgtccgcga
    25981 ctgtcagttt ccagcccaat gttctgtaac gccagcgaat cggtattacc ggtacgaccg
    26041 ttggcataga gcaggcagtc agctttcagt tttttacccg acttcagatg catgatcaca
    26101 ccgtcgtcac agccttcgat cttctcgtac tcttcgttgt gacgaatcac tacgccactg
    26161 ttccagaagt gataggagag agaatctgac atctcttgat cgagaaatgc cagcaggcga
    26221 tcgcgggtgt tgatcagatc cacttttaca tccataccgc ggaagatcga cgcatattca
    26281 cagccgatca ctccagcacc atagataagt acatggcgcg gttcgtggtg catgctgaga
    26341 attgagtcgc tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg atgatatgga
    26401 cgagagccgc aggcaataac aaatttttca gcggttagtg tttcaacgct gccgtccggg
    26461 caatccagcg ccaacgtatg ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag
    26521 tgattacgtt cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac gttatcggca
    26581 tggttaagga tatcggcaaa agaagagcgg agcagtcggg aatggtcgct gtatagtggg
    26641 ttttgattga attctataat gcggctgacg gcgtgacgga gagctttcga cgggatggtg
    26701 ccccagtggg tgcaaccgcc gccaacattt tgataacgct cgataactgc gacgcgcgca
    26761 ccttgcttaa ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc tattactatg
    26821 gcatcgtaat cgtaggaatg tggcatggta gggcttacct gttcttatac ataaaagcaa
    26881 cagaatggta acattttatc gcgggtaagc caattgatcc ccgtcattaa tctggctata
    26941 gcgtgagcgg cctttgcttt gtctgcctcc ttcttaaccc atttattcac tattaatcgc
    27001 tttttattat ggctgatagt tttatcttgc cgaagcgtgc ttttttgtta ttgtctt
//
LOCUS       DAZKFZ010000051        26369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000051 DAZKFZ010000000
VERSION     DAZKFZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(61..1251)
                     /gene="bcr"
                     /locus_tag="ACHOQW_001907"
     CDS             complement(61..1251)
                     /gene="bcr"
                     /locus_tag="ACHOQW_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP9572992.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1279..1974)
                     /gene="rsuA"
                     /locus_tag="ACHOQW_001908"
     CDS             complement(1279..1974)
                     /gene="rsuA"
                     /locus_tag="ACHOQW_001908"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP9572993.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2123..3883
                     /locus_tag="ACHOQW_001909"
     CDS             2123..3883
                     /locus_tag="ACHOQW_001909"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9572994.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            4008..4292
                     /gene="rplY"
                     /locus_tag="ACHOQW_001910"
     CDS             4008..4292
                     /gene="rplY"
                     /locus_tag="ACHOQW_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP9572995.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4431..5438)
                     /gene="yejK"
                     /locus_tag="ACHOQW_001911"
     CDS             complement(4431..5438)
                     /gene="yejK"
                     /locus_tag="ACHOQW_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP9572996.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5620..5847
                     /locus_tag="ACHOQW_001912"
     CDS             5620..5847
                     /locus_tag="ACHOQW_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP9572997.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5867..7627
                     /gene="yejM"
                     /locus_tag="ACHOQW_001913"
     CDS             5867..7627
                     /gene="yejM"
                     /locus_tag="ACHOQW_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP9572998.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7702..7778
                     /locus_tag="ACHOQW_001914"
     tRNA            7702..7778
                     /locus_tag="ACHOQW_001914"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7736..7738,aa:Pro,seq:ggg)
     gene            complement(7879..10470)
                     /gene="yejO"
                     /locus_tag="ACHOQW_001915"
     CDS             complement(7879..10470)
                     /gene="yejO"
                     /locus_tag="ACHOQW_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFP9572999.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            10790..11437
                     /gene="narP"
                     /locus_tag="ACHOQW_001916"
     CDS             10790..11437
                     /gene="narP"
                     /locus_tag="ACHOQW_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP9573000.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(11472..12524)
                     /gene="ccmH"
                     /locus_tag="ACHOQW_001917"
     CDS             complement(11472..12524)
                     /gene="ccmH"
                     /locus_tag="ACHOQW_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP9573001.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12521..13078)
                     /gene="dsbE"
                     /locus_tag="ACHOQW_001918"
     CDS             complement(12521..13078)
                     /gene="dsbE"
                     /locus_tag="ACHOQW_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP9573002.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(13075..15018)
                     /gene="ccmF"
                     /locus_tag="ACHOQW_001919"
     CDS             complement(13075..15018)
                     /gene="ccmF"
                     /locus_tag="ACHOQW_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP9573003.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(15015..15494)
                     /gene="ccmE"
                     /locus_tag="ACHOQW_001920"
     CDS             complement(15015..15494)
                     /gene="ccmE"
                     /locus_tag="ACHOQW_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP9573004.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(15491..15700)
                     /gene="ccmD"
                     /locus_tag="ACHOQW_001921"
     CDS             complement(15491..15700)
                     /gene="ccmD"
                     /locus_tag="ACHOQW_001921"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP9573005.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(15697..16434)
                     /gene="ccmC"
                     /locus_tag="ACHOQW_001922"
     CDS             complement(15697..16434)
                     /gene="ccmC"
                     /locus_tag="ACHOQW_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP9573006.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(16476..17138)
                     /gene="ccmB"
                     /locus_tag="ACHOQW_001923"
     CDS             complement(16476..17138)
                     /gene="ccmB"
                     /locus_tag="ACHOQW_001923"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP9573007.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(17135..17758)
                     /gene="ccmA"
                     /locus_tag="ACHOQW_001924"
     CDS             complement(17135..17758)
                     /gene="ccmA"
                     /locus_tag="ACHOQW_001924"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP9573008.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(17771..18373)
                     /gene="napC"
                     /locus_tag="ACHOQW_001925"
     CDS             complement(17771..18373)
                     /gene="napC"
                     /locus_tag="ACHOQW_001925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP9573009.1"
                     /translation="MGNSDHKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(18383..18832)
                     /gene="napB"
                     /locus_tag="ACHOQW_001926"
     CDS             complement(18383..18832)
                     /gene="napB"
                     /locus_tag="ACHOQW_001926"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP9573010.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(18829..19692)
                     /gene="napH"
                     /locus_tag="ACHOQW_001927"
     CDS             complement(18829..19692)
                     /gene="napH"
                     /locus_tag="ACHOQW_001927"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP9573011.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(19679..20374)
                     /gene="napG"
                     /locus_tag="ACHOQW_001928"
     CDS             complement(19679..20374)
                     /gene="napG"
                     /locus_tag="ACHOQW_001928"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP9573012.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(20381..22867)
                     /gene="napA"
                     /locus_tag="ACHOQW_001929"
     CDS             complement(20381..22867)
                     /gene="napA"
                     /locus_tag="ACHOQW_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP9573013.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(22864..23127)
                     /gene="napD"
                     /locus_tag="ACHOQW_001930"
     CDS             complement(22864..23127)
                     /gene="napD"
                     /locus_tag="ACHOQW_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP9573014.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(23117..23611)
                     /gene="napF"
                     /locus_tag="ACHOQW_001931"
     CDS             complement(23117..23611)
                     /gene="napF"
                     /locus_tag="ACHOQW_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP9573015.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            23720..23884
                     /gene="yojO"
                     /locus_tag="ACHOQW_001932"
     CDS             23720..23884
                     /gene="yojO"
                     /locus_tag="ACHOQW_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP9573016.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            24018..24506
                     /gene="eco"
                     /locus_tag="ACHOQW_001933"
     CDS             24018..24506
                     /gene="eco"
                     /locus_tag="ACHOQW_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP9573017.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(24656..26302)
                     /gene="mqo"
                     /locus_tag="ACHOQW_001934"
     CDS             complement(24656..26302)
                     /gene="mqo"
                     /locus_tag="ACHOQW_001934"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP9573018.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT         6441 a         7459 c         6493 g         5976 t
ORIGIN      
        1 gatttgtggt tgacatgcat caacaaagga agccttttag cttcctcgtt gtgcaataga
       61 tcaccgtttt ttcggccgac tggcgtacag acagaagaga atggagctgg ttgcgcagaa
      121 tgcaattgac caaatcatcg gccatgcaga gttaaaggtc gcaagagaaa gcaatgcgcc
      181 aacaattgcc cctatgccaa aacggaaggt tcctgccagc gaagatgccg ttcccgccat
      241 atggggaaac tcatcaagaa tgaccgccat cgcattggat gacaccatcg acacgcagcc
      301 cacaaacgcc gcaacgccaa ccaccagcga ccaaaatccc agacccagca gcgcactgat
      361 gaccatccac gctgccataa taaattgtat ccacaacccc gagcggaaca tatttaacgc
      421 gccaatacgg cggacgaagc ggctattaaa gatggtcatc acgaacagga aaacaatgtt
      481 tagtgcaaag taataaccaa agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa
      541 cggtccggcg cttaagaacg agaacatccc ggcaaagctg aaaccactgg ccagcatata
      601 actcaggaca cgcttatgac ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg
      661 aaaaggctgg cgacgctccg gtggtaaggt ttctttgatc agaaagaaga tcattgccga
      721 agccagaatc gccgccacgg cgaggatcca gaagatgtaa tgccagctca gccacaccag
      781 cacccagccg ccaactatcg gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa
      841 cgacatcatc cgcgagaact cttctttcgg gtagatatca cgcatcaaag cgttgataac
      901 cacgctggcc gcagccgccg ccagcccgtg gaagaaacgc atcacaatca gctgatcgat
      961 ggtttgtgcc aacgcacacg ccaccgcggc ggcggcaaac accagcgtac cgccaagaac
     1021 caccggctta cgcccgaagc tgtctgccat cggcccgtag attaactgcc ccaacgcaaa
     1081 gcccagaata taagtactga gggtcatctg cgtactgccc gccggtacgc caaactgcgc
     1141 tgaaattacc ggtagcgcgg gcagatacat atcaatcgac agcggcatca acatggccag
     1201 caggccaagg ataaaaacaa tagcaaacga cgaatgctgt cgggtggtca caacgggctc
     1261 ctgaaagtca ttgaaaagtt agacgacgct ggcaatttct tcttcagtta acggacgata
     1321 ttcaccgggg gctaaatcag catccagcgt aatgccgcca atacgttcac gatgcagctc
     1381 aaccacatgg ttacccacgg cggcgaacat gcgtttcacc tgatgataac gcccttcgct
     1441 gatggtcaga cgaacctgcg ttggggtaat cacttcaagc accgctggct tagtgagatc
     1501 tttttcgtta tgcagctgca cgcctttagc aaattgctct gccgtatcgt cagctacggg
     1561 tgattccagc gtcaccagat aggtcttctc gcaatgatgg cgcggagaag taatgcggtg
     1621 cgaccactga ccatcatctg tcattagtac cagaccggtg gtatcgatat ccagccgccc
     1681 tgcggcgtgc agtttccacg ccactggttc atctagaaaa tagagcaccg ttgggtgatc
     1741 agggtcgtcc gtggagcaaa catagccctg aggcttattg agcatgaagt aacgtggacc
     1801 gtgttgctgc gccagcgggt tgccatcgta agcgacatca tgttcaggaa gcagtttgaa
     1861 cgctgcatta cggacgattt cgccatcgac ggtgacacga ttgccgcgga tttcacgccc
     1921 ggcaatagca cggctaacgc cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt
     1981 gattttgcct gtaaaaacgg aggtcgggca ttgacccgaa aatctgaact gttgtctgcc
     2041 cagtatagcg gtctaataac ttccctcaag ggaaaaagaa ccgtggcata ctattagcag
     2101 aataatctac ctacgcgaga ccatgatttt tacactccgc ccatatcagc aagaagccgt
     2161 ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg tgctgcccac
     2221 cggtgcaggt aaaagcctgg tgatagcgga acttgcgcgg ctggctcgtg gtcgcgtgct
     2281 ggtgctggca cacgttaaag aactggtggc gcaaaaccat gcaaagtatc aggcgctggg
     2341 gctggaagcc gatatttttg ccgccgggct aaagcgcaaa gagagccacg gtaaagtggt
     2401 atttggcagc gtgcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg aattttcgct
     2461 gttgattgtc gatgaatgtc accgtattgg tgacgatgaa gagagccagt atcagcaaat
     2521 cctcactcac ctgacaaaag tgaatcccca cttacgcctg ctggggctga ctgccacgcc
     2581 ttttcgactg ggcaaaggct ggatctacca gttccattat cacggcatgg tacgcggcga
     2641 tgagaaagcc cttttccgtg actgcattta tgaactgccg ctgcgttata tgattaaaca
     2701 cggctatctg acgccgccag aacgactgga tatgccagta gtgcaatacg atttcagccg
     2761 cttgcaggca cagagtaacg ggctattcag cgaagccgat ctcaaccgcg agctaaaaaa
     2821 acaacaacgt attaccccgc acatcatcag ccagattatg gagtttgctg caacgcgcaa
     2881 aggggtgatg attttcgccg ccaccgttga acacgcaaaa gagattgtgg gattactacc
     2941 cgccgaagat gctgcactga ttactggcga cacccccggc gctgagcgcg atgtgttaat
     3001 tgaagatttt aaagcccagc gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg
     3061 atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat cagtgagtct
     3121 ttaccaacaa attgtcgggc gcggtctgcg tctcgctcct ggcaagactg attgcttaat
     3181 tcttgattat gcgggtaatc ctcacgatct ctacgcgccg gaagttggta caccgaaagg
     3241 caaaagtgac aacgttccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt
     3301 ttgggggaaa acgaccgccg acgggacatt gattgaacac tttggtcgcc gctgtcaggg
     3361 atggtttgaa gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc gttttaaaaa
     3421 ttgcccgcaa tgtaacgcag aaaatgatat tgccgctcgc cgctgccgcg aatgtgacac
     3481 ggtactggtt gatccggatg atatgttaaa agcggcgcta cgactgaaag atgcgctggt
     3541 attacgctgt agcggcatgt ctttgcagca tgggcacgac gagaaaggcg aatggttgaa
     3601 aatcacctat tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc
     3661 cgcccagcga actgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat
     3721 ccctctgcgc tggatcaccg ccgccgatat cctcgcccag caagccttat tgcgacaccc
     3781 ggattttgtc gttgcccgca tgaaaggtca gtactggcaa gtgcgtgaaa aagtgttcga
     3841 ttacgaaggt cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat
     3901 gtataaggcg attgagtata gaatctcgcc cgcttttgca tacgcaaagc agatcactta
     3961 cctgttgctg ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca
     4021 acgcagaagt acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca
     4081 agttcccggc aatcatctac ggtggcaaag aagcaccgct ggctatcgag ctggatcacg
     4141 acaaagtcat gaacattcaa gctaaagctg aattctacag cgaagttctg accatcgttg
     4201 ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga
     4261 agctgcagca catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc
     4321 tccggcgggg ttttttgtat ctgcagatta tgcctgatta cgatattgct attttttgca
     4381 ggccagataa ggcgttcacg ccgcatctgg cattcataac acaaaaccga ttaattgccg
     4441 ccggacgtcc ggcgctgcaa ttgatcgcgc aaattcggcg gcgtgccttt aatagtcagg
     4501 gtatcggtcg ccggatccca gaaaatccgt tcgcccaaca gcatcgcatc aaaattaata
     4561 gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc
     4621 gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg
     4681 ctgacgcctg ccagctcttt cgacagcgat tccagttcga tctcttcgcc tgcctgcagc
     4741 tgctcattac agtagctata aacctgctgg cgcacgttct gtcgctctgc tttatccagt
     4801 tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc cacggttttg cgctttcgcg
     4861 ttcaatcctt cgctggcccc gaggaaatcc ataaagaaat ccgccacttt gcgccctact
     4921 cgccctttca ggaaagtgag atagcgggtc gactctggat tggtttccca ttcggttaaa
     4981 tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgggttgg gttgatgtcg
     5041 agattttcgt taacgcgcat actgctcagg ttgctcagga ctgccaccag cagatactcc
     5101 accgccagat aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc
     5161 gctaattcgt cacgcaggcg accggttgcc gcgcggctaa atgccagaaa gtcctcttca
     5221 ccctgacgct gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa
     5281 gctttatttt tggcgctata gacccgatgc agttctgcta ccatctcaac gacggtttct
     5341 gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata
     5401 agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg
     5461 ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc attttacaga
     5521 aggcggcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg
     5581 taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac
     5641 agtgatgaac aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag
     5701 gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc
     5761 agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc
     5821 tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg
     5881 tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt
     5941 caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac
     6001 cacgcttgct ggtcgcattt attcctacgt aagcattatc ggccatttca gcttcctggt
     6061 gttcgccacc tacttgctga ttctcttccc gctgaccttt atcgtcggct cccagaggct
     6121 gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg acgctattac tgatcgatag
     6181 cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcaac tggttattaa
     6241 cccagacgaa aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg tgccggttat
     6301 tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg
     6361 tcgtcggcgc ttcgcgcgtc cgctggccgc attcttattt atcgccttta tcgcctcgca
     6421 tgtggtgtat atctgggccg atgccaactt ctatcgccct atcaccatgc agcgcgctaa
     6481 cctgccgctt tcgtacccga tgacggcgcg acgttttctt gagaagcatg gcctgcttga
     6541 tgcgcaggag tatcaacgcc gtcttattga gcaaggtaat ccagacgccg tttccgttca
     6601 gtatccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg tcttgttgat
     6661 tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt
     6721 tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc ggcaacacta cagacaacgg
     6781 catctttggc ctgttctatg gcatctcgcc gagctatatg gacggcattc tgtcgacccg
     6841 tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg ggttattctc
     6901 atcagatggc tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc
     6961 gagcgtacgc acccaatccg acgagcagac cgccacgcag tggatcaact ggctgggacg
     7021 ctacgcacaa gaagataacc gctggttctc gtgggtctct ttcaatggta ctaacattga
     7081 cgacagcaat cagcaggcat ttgcacggaa atatagccgg gcggcaggca atgtcgatga
     7141 ccagatcaac cgcgtgctca atgcactgcg tgattctggc aaactggaca atacggtggt
     7201 gattatcact gccggtcggg gtattccact gagcgaagag gaagaaacct ttgactggtc
     7261 ccacggtcat ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat
     7321 taatgcgctg actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctacatgt
     7381 cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgtca
     7441 ttactgggtt accgcagcgg ataacgatac gctggcaatt accaccccga aaaagacgct
     7501 ggtgctgaac aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga
     7561 tgaaaaacca cagttaagtt tgttattgca agtactgaca gacgagaagc gttttatcgc
     7621 taactgatta attataaatc agttagcgaa atatcttact tgcaatcggt gtggaaaacg
     7681 gtagtattaa caaccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt
     7741 gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaaa aaaccaaccg
     7801 taaaggttgg tttttttata tctgcattta attcgataaa cagaccgcga cacatcaggg
     7861 cctgtttatt gtctgttatc agaacgtcca gaccacaccc gcctgggtat tccacggcga
     7921 ttctacccct gcgccattgc catagctgac tgacaaatga ccgcttaacg tcggggtaaa
     7981 cgatgacctt atcccagcct gataaacgcc acgggttcca gccacatcat tattaaattt
     8041 gccatcgtca ttaactttca cctggttaga atcggcgtat tcctgacgca cggcggcttt
     8101 cagccagggt tccagcgtcg taccgttttg caggtccatg tgatagctta ccgccgttcc
     8161 cgcttcagcg cgtaatatcc gggtatttcc cacatccgcg cgcatgccgt ttgataacgt
     8221 gtagtcctga ccatctgtgg taaagccggt aaaggccaga tagggtctaa cactccacaa
     8281 tccgtcaacc caacggaacc cgctctcgac atgagcaccc gcgccgttac tattgtaatc
     8341 gccaaacgct gttgccccat tactcatctt gccatggatg gtgttggcaa aacggtcaac
     8401 tttcaccact ccatcaacat aggcaccgtt ctgatgctcc caaccggcat aagcccccag
     8461 ggtatagcta tcgacattgc ctttgccgcc gcgatcaaaa ccaatatcag aatgagagta
     8521 accaaagaac aagccgcgaa ttgtgctgct ttcttcacgg gagaaacggc tatcgatacc
     8581 gagcgtcagg cccgtcaatg tttgctcaaa accagctccc gcatcagtgg tcacgttgtt
     8641 gcgggtgtta attgccgaac tccacatcgc cgtatcgtaa ctaacgcctt ttacgctacc
     8701 aagacgctca cgcacggtgt ccagttctgc atcaaatacc agcggttgtg cggccgccat
     8761 attcagcaca tcagtggttg aaggggtaat ttgcgcgcga ttctctgcca gactccagct
     8821 atggttacca ttatccagca aggtatattc atacgtaccg atatcaacaa cgcctccggc
     8881 attgcccaac gtaaatgcag catcaccgcc gcccgttgtt accagtgtaa ggctatctcc
     8941 tgctgccggg ctggcaccgg tgtccgtcac gaatattttg aaatcacctg ttgcctgacc
     9001 ggtgacgttg agctgatcgc tctgatgatt cgccatatcg gtacgcatat aaaaattgcc
     9061 gtttcccgac agggtattgg tggtcaacgt agtgaaattt tccgcacttg ccgtcacaga
     9121 tgaacgagtc actgactcat ccatcataat cacatttgcg ccagacatcg caaggttggc
     9181 taagttaagg cgggtgtttt ggttcgtcgt agtgatatcg gcaatgatgg tccatgcgct
     9241 attttcgagc gaaacatccg ctttgctggt atccacggca ccatgcgttc ttaaagaagc
     9301 gccctcagaa accactacct tacccaccaa cattgcgggt gcataattta tttgcggctt
     9361 cataacctca agaatgccat catttcctct gactgacacg ttaaccattg tgccagccca
     9421 gacgttagcg cggccattgt tgataaccat attttcagag atggctttag aggaatacgt
     9481 tatacttcca ttctgatctg atcgaccgag atcatatacc gcatttgcct gcactctcgt
     9541 accagtatcc tttcccagcg attgcatagt ggcatgctca tcgaggatag tgtcaatggc
     9601 ctgcgtgtcc tccataacaa taagcccgga accatcatcc agttcataat tttttgacac
     9661 accatctttt atactaaatt ggcctttact gtttgtacca ctcacttcca gcgcattcgt
     9721 tgagacaatt aattttccgc ctgctttttt atcaacgcca gttgcagtcc cgccatccat
     9781 aacctccagt aagccgccac tatctacagt agtattataa gcgaagtcat tttcttcaac
     9841 tcgcaaactt ccgccgtttt ccaacagcat attgttagca ataccattct tgatttcgaa
     9901 ctgaccgaga cggtttgttc cgaatacctc catgacccgc gtgcttgctt taatagcacc
     9961 gcctgctttc tgatctaccg caatcgctaa tcccccctct ttaacctcca gtaatcctcc
    10021 cttttcgata gcggcaagga cggtaatccc atctttttca acgattaagc gcccgccttt
    10081 aatcgtggga tcattcgctg cgccataaac ctgtagcaca ccattattga gcgtagtctg
    10141 gtttgccgcg ccacccattt gaacaatttg ataaccatca tttaatactg agtattctgc
    10201 ggtgccattc atttcaatgt actgataccc gccgttaatt tcagtattac tacttatacc
    10261 aaattcttta atatgctgtt ctccgccagg attgattttc gtattattgg cggtcccata
    10321 aaccagctgg atatcttttt ccaggacaac accatcaact gtctcaccat actcaactgt
    10381 tgatgccaat gctatgggtg aatataacgc tgtagctacc agaacagata ttgcggaacg
    10441 acaaagagaa acagaaccag attgatgcat tgagctttca tcctatgaaa ttaattgcag
    10501 ttaaaagcat tgggtacaga aaatacccat agctccatac ccggagtcag tttttaaaaa
    10561 ctgtttaaag aaatgcacaa gtattgtgat tgatttttta gttgtttttc ttgatgagaa
    10621 gctgatgcaa aattccgtct ttataatgaa aatgatgcca aagcgaacga caaggttgta
    10681 gttttcgcta catgtccata cataaaatgg ggtaacattc acgcgcctgg tagcgttacc
    10741 aacgctacgc tcaaacataa tgattctaat aaaacctcag gagactacta tgcctgaagc
    10801 aacgcctttt caggtgatga ttgtggatga tcatccactt atgcgacgcg gtgttcgtca
    10861 gttactggag cttgatcctg gctttgaagt ggtcgccgaa gcgggcgacg gcgcgagtgc
    10921 tatcgatctg gcgaatagac tggatatcga cgtgatcttg ctggatctca atatgaaagg
    10981 tatgagtggc ctggataccc tcaatgcctt gcgcagggat ggcgttaccg cgcaaattat
    11041 tatcctgacc gtatcagatg cctccagcga tgtctttgca ctgatagacg caggcgcaga
    11101 cggttatctg ttgaaagaca gcgacccgga agtgttgctg gaagcgattc gtgccggagc
    11161 gaaaggcagc aaagtcttta gcgaacgcgt caatcagtac ttacatgaac gtgaaatgtt
    11221 tggcgcggaa gaagatccct tcagcgtgct gacggagcgc gagctggatg ttctgcacga
    11281 gctggcacag gggctgtcaa ataaacagat tgcctcggtg ttgaatattt ccgagcagac
    11341 agtaaaagta catattcgca atctactgcg caaactcaat gtccgctcac gcgtggcagc
    11401 caccattctg ttcctgcaac aacgcggggc acaataaaaa tagcccgatg gcatcaccat
    11461 cgggcctctt tttatttact ctcctgcggc gacaaatgtt gcatcgcctg cgcgatacta
    11521 cgttcaatca ccgcacggcg agtatcgttg gcaggtaaga gtttcaacat catctcccac
    11581 gcggcaacgg cttcgccaaa tcgctgctgc tcaaaggcat taaacgcata catgcttaac
    11641 acacgaatat tgctatggtc cgttctcacc aactggcgca gcagttcgcc gccgaggcgg
    11701 ttgtcgttgg gatcagatga acgtgtcaac gcttcagcat aacccagtgt ggcatcgctg
    11761 tttttcggat cgaggcgata cgcagtagcg tatgcatcgg tggcgatact ggcgttaccc
    11821 agcgccatgc caacgcgacc caacataatc caaccttcta tatctcccgg atttttttgc
    11881 agttgagtac gcatccccag cgcaaggcgc gacatctctt cttcgttgag cggatcggct
    11941 ttcggatcca gcgccctgtc cagcaacgcc ggagcctgtg ccgtggcctg ctgccagatt
    12001 ttcacctgct gataattgcc agtctggtag tagctgacgc cagccacaat taacgccacc
    12061 acaatacccg gcagataaac aacatatccg gcacgcttac cttccggcac gctttgttca
    12121 ggaaacgctt ccggtaccac gcgtacccga cgccgcgaac gggcgtatat gacccaaccg
    12181 ccaataccaa tagccactac cggaagcacc cacagcagca cggtcagcgg cgttaacggc
    12241 ggatcgtaag tgacgaagtt gccgtaacgc gccaccatat aatcgacaat ctctttctta
    12301 cttttgcctt cctgcatcag ttcatacact ttctggcgca ggtcggtggc aatcatcgag
    12361 ttggaatcgg caatgctgtt attctggcat ttcgggcagc gcagttcttc agtgagctga
    12421 cggaactgtt gttcctgtgc ttcatcttta aactgcaaca cgtcgatggt cgccagcgct
    12481 gagccggaga tcatcagcat cagcacgccc aataaaaacc tcattgtgcg gcctccttac
    12541 tgtatttctc ccacagcggc ttgatctctt cttcccagac gcgaggattg agatcgcccg
    12601 catggcgata gcgaatgatg ccgttgccgt caataagaaa cgtttctggc gcgccataga
    12661 caccgagatc cagccctaac atgccatcgc catcaaaaag gcttagcgcg taaggattgc
    12721 ccagctcttt cagccagctg attgccttct ggcgatcgtc tttatagttc atgccgacca
    12781 cgcggatgcc ctgcgcagaa agctgattca gatattgatg ttccgcacgg caggtcggac
    12841 accaggtcgc ccagacgtta agcagcactg gtttgccctg agtcagcaca tccgcctgat
    12901 aaaactgccc cggattgtcc agtgattcga gacgaaactt cggcacaggc ttgccaatga
    12961 gcgccgattc cagattggtc ggatcatccc cttcggcatt acgcgccagc tgccacagca
    13021 gcgccgcggc aatcgccagg aagataatca acggaattaa caatactttg cgcttcatac
    13081 agcctccggc gcagtttttt gcgggctcac gcgcttacga tagcgaggat caaacagaca
    13141 cagcagtccg cccaacgcca tcatcagccc gcccgcccaa atccagcgaa caaatggttt
    13201 gtagtaaaga cgcacagccc acgcgccgtt ttccagctct tcaccgagcg cggcgtacag
    13261 gtcacgcgtg atgccgccgt caattgccgc ttcggtcatc atcgacccgg cagtgttgta
    13321 ataacgtttt tccgcataca gcaccgtttc cggtttaccc tctcgcgtta cgccgatagt
    13381 cgccacaccg ccacgccagt tcgggccagt cacctctttg acatcacgga aggtgaagcg
    13441 atattcatga atatcgacgc tatcgccgga cttcatgcgc acatcacgct caacgctata
    13501 gttctggcta aaggcaatgc caacaattgt cactgccagc cccaggtgag ccgccaccat
    13561 cccccaataa ctgaaggtgg ttttcgtgcc gcgtgaaata cgtagcgcag cttccgcaat
    13621 tgccagcacc gcaatccagc aggccattgc caggccgagc accgtcatcg ccacaacttt
    13681 gctttcgaac agccacggca acagcagaga cagcaccagc gtagagatga aggcgataat
    13741 caataaattg cggattttac gtgggcgatc gcgcccccag cgcaccagag gaccgacacc
    13801 cagcagtagc gcaaacggca ccatcagcca ggtaaacatg gtgttgaaga acggttcgcc
    13861 aatcgaaata ctgcccagtc ccagttgctt gtgcaccagc ggcagcagcg tccccagcaa
    13921 caccaccagc atcgcggcga ccagcaaaac gttgttcgct aacagcaaag attcccgcga
    13981 ccacagcgca ttgtttacgc gtgagcgaac tttgtgtcca cgcgcggcaa acagcagcag
    14041 cgaaccgcca atcaccagca ccataaaggc gaggataaac ataccgcgcg ccggatcaga
    14101 cgcaaacgcg tgtaccgata ccagcacgcc ggaacgcacg aggaaagtcc ccaacagaca
    14161 caacgagaag gcactgattg ccagcagtaa tgtccacgct ttgaagctgg cgcgttgttc
    14221 agtgaccgcc agtgagtgca tcagcgcagt ccccaccagc cacggcataa acgaggcgtt
    14281 ttctaccgga tcccagaacc accagccacc ccagccgagt tcgtaatagg cccatgcgga
    14341 accgagcacg atgccgagcg tcaggaagat ccacgctgcc agcgtccacg gacgagtaaa
    14401 acgcgcataa gtgctgtcca gacgcccgct cagcaaagaa gcaatggcaa aagcaaacgc
    14461 caccgagaaa cccacgtacc ccatataaag cagaggcgga tggaagatca gccccggatc
    14521 ctgtaacagc gggttaagat cgcgcccttc aatcgggaag ttcggcagcg tgcgtgagaa
    14581 tgggttagag gtaaagagaa tgaacagcaa aaagccgaca ctgaccatcc ccattatcgc
    14641 cagtacgcgg gccacaatat ccagcggaat acgctgacta aaaatcgcca ccgcaaaggt
    14701 ccagccgctc atcagcagca cccacagcag cagtgagcct tcatgtgcgc cccaggtagc
    14761 cgccacgcga taccacaccg gaagctgggt attggagttg ctggcaacat aggtgacggt
    14821 aaagtcgttg accacgaaag cattgaccag caccagaaat gcgccagcca cagacataaa
    14881 cagcagccag gcaaacaagc gggaagacgc catcatgcgc gcatctccgc gcgccacgcc
    14941 ccatagcgga tacacggaca gcagcagcgc aattcccagc gccaggcaca gcagcccgtt
    15001 accaatttcc ggcatcatga tgctgggtcc ttataaacac tcgccgggcg acggtggtta
    15061 gcttccatcg ctttctcaac ttctggcggc gtgtagtttt catcatgttt cgccagcact
    15121 tcttttgcga ggatatgatt gcctttttcc agctcgccct gcaccacaac gccctgccct
    15181 tcacggaaca gatccggcaa aatgccttcg taagagacat ccactgagcc ttcagcgtcg
    15241 taaatggtga aggtcacttt cagcgaattg ggatcgcgct gcacactacc cggcatcacc
    15301 atcccgccaa cgcgcagacg ctgaccgact tccggcatct gctgagtttc acgcttgccg
    15361 tagagaattt cccccggcgt ataaaagaga tcgatattcg agcgcagcgc atatagcacc
    15421 agaccgatag tcagcgccag ccctgccaac acggcacagg caatccacaa gcggttttta
    15481 cggcgaatat tcatgcagcc tcctgctgtt gcgcagcacg taaacgcgcc tcacgcgccc
    15541 gctgttgcgc cacaccacgc agaattgcgc gatgctgcat caccgagtgc acgaccaaaa
    15601 ccaccagcgg aataacggtc atcaccaccg ccagccagac aaaaaaggcg taaccgccca
    15661 ttgcgaaaaa ttcattccag gaagcaaatg caggggtcat ttacggcctc ttttcagtat
    15721 cagttcactc acccacggac ggcgtttttc catcagcaaa atcaaattac gcatccgcat
    15781 cagcgtcagc gtggcagaga ggagcaggaa gccaaaaatc gaccagcgca gcggcgaacg
    15841 catcgccgga tcgatacttt gctgcatccg cgttgatccc tgatgcaggg tgttccacca
    15901 ctccacggag taatgaataa tcggcagatt caccacgcca atcagcacca ggatacctgc
    15961 cgcacggccc gccagacggc ggtcgtcgaa ggcgtgccac agggcaatca cacccacata
    16021 caaaaacagc agcaccagtt cagaagtcag acgtgcatcc catacccacc aggtgcccca
    16081 catcggtttt ccccatgcag agccggtaac cagggcaata aaggtaaaca cggcaccaat
    16141 gggggccatc gccgccaccg ccaggttggc cattttcatc tgccagacaa ggccaataaa
    16201 cgccgccacc gccattgatg cataaatgcc catcgaccag atcgccgcag gcacatgcag
    16261 gtagataatg cggtagctat ttccctgctg ataatcagcc ggagcaaagc cgaatcccca
    16321 gatccagccg acggtaagca cgactacact ggcaattgcc agccacggta taaaccagcc
    16381 acagatttga tacagccgtg gtgggatcgc cagttgatgc agtgttttcc acatagtttc
    16441 gataccagac tcgaacaaaa atcagtaatc cagcgttatt gaatgctgat tcgtaacgct
    16501 gccgccgtcg caaaaggact taatgtcgcg gtgcctgcca gcaacgcgcc taaaattgcc
    16561 agatacccgt caacgggcaa atgcatagaa gccgcgtcca tcgcggcggt ggcaaagatg
    16621 agtaatggga tagtcagcgg taacaccagt atgctgagca gcacaccacc gcgcttaagt
    16681 cccactgtca gcgccacgcc cggtgcaccg agaaagccaa gcgtaggcgt tcccagcagt
    16741 agcgttaacg ccatcacttg ccagccataa acatccattc ccagtagcat tgctaccagt
    16801 ggcgaaagga tgagtaacgg cagaccggtt accatccagt gcgccatcac cttcgccagc
    16861 acaacggcgg gcaagggtaa cggcaacaac atcaattgtt caagactgcc gtcctgcaaa
    16921 tcgtcacgga acagtcgttc cagcgccagc aaggatgaaa gcagcgcggc aacccagata
    16981 atgcccggtg caatacgtgc cagcagttgc ggctccggac cgatactgag cggaaaaagg
    17041 gtaattacaa tcaggaagaa ccacagcggg ttggcgattt cagcgctatg gcgaaacgct
    17101 acgcgcagct caagacggaa aatgcgccag aacatcatac ggccctcgtt tgcgtcagtg
    17161 aaatgcggcg aattttactt tcagcaacgt tgagcggctg gtgggtagtg agaatcacaa
    17221 tccccccctg ctctgtatgc tgcgccatac gctgggtcag acgatcgacg ccgttaacgt
    17281 caatcgcggt aaaaggctcg tcgaggatcc ataacgtggc acgggtcagc cacagacgcg
    17341 ctaaagcgac gcggcgttgt tgcccggcag aaagttgatt taccggaata tcttcgaatc
    17401 cggcaagccc ggcctgcgcc agggcttcca gacattgtgc ggtatcgcca tcgcgatgat
    17461 aaaagtgcag attttctaac gccgtcagcc gggttttgat ccccggctga tggcctatcc
    17521 ataacaggtt ttgatggtag ctgtcgcgta cctgatgcaa gggctgccct tgccagagaa
    17581 cctcgcctgc gtcagggcga gacaaccccg tcagcaaacg gagaagcgtt gtcttccccg
    17641 cgccgttgct accggtgatt tgtacccact ctcctgcgtt cagcgtaaat gacaagccac
    17701 taaataaggt tcgttcatcc cgctcacaaa gtaactctct gacttcaagc atacccacgc
    17761 aataaccctg ttaaaaacct ggctcgactt cacgcatatc gggcagcttg tgcgctatcc
    17821 ctttatggca atcaatacag gtttgcccat ctttcaccgc ctggtcatgc atcttcgcgg
    17881 caaccgattt ctgggcggtt gtatccatat actcgaagtt gtgacagtta cggcactcct
    17941 gcgagttatt gtccttcatg cgccgccact cattctgtgc catcgtcaga cgatgagctt
    18001 caaatttctg cggcgtgtca ataacgccaa aaattttacc atacagctct ttacttgctt
    18061 tgagcttgcg tatcatcttc ggcacaaact cgtgcggaac gtgacaatcc ggacaggtcg
    18121 cacggacgcc gctacggttg ttgtagtgca cggaatccat gtattcctga tacaccgtgt
    18181 tacgcatttc gtggcagcta atgcagaact cttcggtatt ggctttttcc atcccagtgt
    18241 taaagccgcc ccagaagacg atgccgccga caaaaccgat caacagcagc gtccccagcg
    18301 ccagacggct gggggtacgc caccatttcc acaggcgctt aatcagacca ggcttatggt
    18361 cagaatttcc cataataacc tcttatttcc cgtaaccttt tgatggggta aaggtattcc
    18421 ccacgattgg cgcggtatcg gcctgcggta cgtgacattg cagacagaaa taacgacgcg
    18481 gagccacttc cgcgcccact ttgccgtcgc tgtccataaa gtgagtagga ctgatacgcg
    18541 gcgcgccagt ggtgcgatag ctttcgacac cgtggcactg caagcagcgg ttggtattgg
    18601 tcgttacctg ataaccttca acgctatgcg ggatcattgg cggctgattc acatagttca
    18661 gcggcatccg atcctgctct tttggcatcc gaatggcccc ttcctgcgtc ccggatactt
    18721 ccggcgactg gctaaaatcg acgccattag ccgcccaaac cgcgccgctt accaccaggg
    18781 ccagcatcgc cgtccattga cacagcgctt tcttcaggtc atggcttttc atgatttcgc
    18841 tcccgaactc catcgtgtag ttattgtaaa tacatcctca gaacagacat ccacgcagcg
    18901 accgcacgtc atgcaatcgc ggctggtgac ctgcaccggg ctttgttcat ccagcaccgg
    18961 ggcacgtagc acatgcggtt ccgggcaaac atgaaaacaa tccatacagc ggttacattt
    19021 ctgccgatcg ctggccgcaa cggtaatcac acctttgcta cccagcacgc catacagcgc
    19081 gccaaccgga caaatgtgcc cgcaccagcc gtgttcaacg accagtagat caaataaaaa
    19141 cagcgcgaga ataagcagcg cgccgctgcc gaagcccatt accaggctac gccccatcag
    19201 ggaaacgggg ttgatccatt cccagatgag cgtgccagtt aaggctgatc ccaccagaat
    19261 gaccaccaac agcacgtagc gaatgtggcg agggatcgtc gcagactgat tgaggtcaaa
    19321 gcgcctgcgt aaccagttcg ctaagtcggt aatcgggttc agcgggcaga cccagctgca
    19381 aaataatcgc tttcctgcca gggcgtagag cacggtgata atcaccgcac cggtcaacgc
    19441 caccgtggca ggcagatgac cactggcaag actttgcagc gtcatcagcg gatcggtcag
    19501 cggaacggtg tcgaataaca ggctactgct atagttgccg tgcaagatcc acacaccaaa
    19561 ccacggaccg ctcaaaaaca tccccagcac gaagaactga caaaggcgac gtaacaccag
    19621 ccaacggtga ctgcgccacc agcctttttt ctccagcgcc tcgcgcccgg cgtcacgttt
    19681 acgatttgcc attgttcccc tccagccagc cgaagcggta atggtgacct aactcccctt
    19741 tcgccagtga cagcggtaac accttgattg ccggttgttc cagcacgcat actttttcgc
    19801 acttaccgca accggtacag gcgtcgctgt gaaccgtcgg cagaaagcgg gcgtgcttac
    19861 cggtacgcgt gttgcgctcc agctccaggg tgatggcctc atcaattttc gggcattcgc
    19921 gataacaaac atcgcagcgc agcccctgaa agttgagaca gttttcctgg tccaccagta
    19981 ccaccagccc catccgcgcg tcgtcgatcg attcaatctc acgatccagc gcaccgctcg
    20041 ggcacacttt ggcgcacgga atgtcctcac acatttcgca aggaatatcc cgtgcgacaa
    20101 aatatggcgt gcccgccgac aggccagagg ccagcgtcgc cagttttaag gtgtcgtaag
    20161 ggcaagcctg aacacactga ccgcaacgca cacaggcact ggcaaaggcg ttctcgttta
    20221 tggctccggg cgggcgcaac cgcacgccag atgcgcgtgc ggtttgctgt tgtaacccca
    20281 gcgccacacc cacggcagcc agcccgcctg ctgtgcgaac gacatcgcgc agaaagcggc
    20341 ggcgaccatt ttgaggtttc gctgaccggg acataatggc ttacaccttc tccagtttga
    20401 ccgcgcactt cttgaagtcc gtctctttcg agagcggatc ggtcgcgtcc agcgttagtt
    20461 tgttaaccag ctgtgcggcg tcgaagaacg gcatgtacac caggccctgc ggcggacggt
    20521 tacgaccgcg cgtttcgaca atcgagatca cttcgccacg gcgagaaacc actttcactt
    20581 tgtcgccacg gcgcagatcg cgcgctttcg catccagcgg gtgaataaac aggaccgctt
    20641 ccgggaaggc acggtgcagt tccggcacac ggcgagtcat actgccggtg tgccagtgct
    20701 ccaggacgcg tccggtagag agccacaggt cgtactcttc atccggtgct tccgccgccg
    20761 gttcgaacgg cagcgcgaag atcaccgctt taccatccgg tttaccgtag aacttatagc
    20821 cttcgcccgc tttcacgtac gggtcgttac cttcgctgta acgccactgc gtttctttac
    20881 cgttcaccac cggccagcgc agaccgcgcg ctttgtggta gtcatcgaac ggcgcgagat
    20941 cgtgaccgtg accgcgacca aaccatgcgt actcttcgaa cagccctttt tgcagataga
    21001 agcccagctc gcgggattca tcgttcagct gatcttccgc cagttcggat accgggaatt
    21061 tgctcacttc gggggtggca tacagaactt cgtacagcgt tttgccacgc agttccggtt
    21121 tcttcgccag cagctcttcc ggccatactt cttcagtttt gaagcggcgg gagaactgga
    21181 ctaactgcca gaggtccgat ttcgcttcgc ctggcgcctg tacctgctga cgccagaact
    21241 gagtacggcg ttcggcgtta ccgtaagccc cctctttttc tacccacatt gcggtcggca
    21301 ggatcaagtc ggcggccagc gcactgactg tcggatacgg atcggagacg atgatgaagt
    21361 tgcgcggatc gcgccagccc ggcatacgct cttcattaat gttcggcccg gcctgcatgt
    21421 tgttggtaca catggtccag taaacattca gcttgccgtc tttcagcgca cggtcttgtg
    21481 ctaccgcatg cagaccgatt ttcgccggaa tggtgccgct cgggatattc cacttcttct
    21541 cgcagatatc acgatgtttc tcgttagtta ccaccatgtc cgcaggcaga cggtgagcaa
    21601 aggtgcccac ttcacgcgca gtaccacacg cggaaggctg cccggtcagg gagaacggac
    21661 cgcaacccgg ctgggaaatt ttgccggtca gcaggtgcag gttgtagacc aggttgttgg
    21721 cccacacgcc acgagtatgc tggttgaagc ccatcgtcca gtaggagatg actttcttgt
    21781 tcggatcggc atacagctgc gccagttgtt ccagctgatc ttttggtacg ccagtcattt
    21841 cagcggtttt ttccagcgta tactcagcaa cgaaggcttt gtaatcttca aagctcatcg
    21901 gttcggaggc gtcagaaccc ggattcttcg ctgctttttc cagcggatgg gtcggacgta
    21961 aaccgtagcc gatgtccgtc gcccctttgc gcaggttaac gtgcttgctg aagaagtcct
    22021 gatttatcgc attgttttga atgatatagt tggcgatgta gttcaggatc accaggtcag
    22081 attgcggcgt aaagatgatg ccgttatccg ccagctcgaa gctacgatgc tggtaggttg
    22141 aaagcaccgc cacggtgacg tcctggttag agagacgacg gttagtgatg cgtgaccaga
    22201 ggatcgggtg catctccgcc atgtttgcgc cccacagcac aaacgcgtca gcctgctcga
    22261 tgtcgtcata gcagcccatc ggctcatcca taccaaaggt tcgcataaag ccaactactg
    22321 ccgacgccat acagtgacgc gcattcgggt cgatgttgtt cgaacggaag cccgctttaa
    22381 acagcttgga cgcggcataa ccttcccaga tagtccactg accagaaccg aacataccga
    22441 tcgattccgg ccctttttct ttcagggcgg ttttgaattt ctcttccatc acatcgaagg
    22501 cctgatccca ggtgattggg gtaaattcgc cttctttgtc atatttaccg tttttcatac
    22561 gcagcagcgg ctgcgtcaaa cggtctttac cgtacatgat cttgggcagg aaatagccct
    22621 taatgcagtt caggccacgg ttaaccggtg cgtccgggtc gccctgacag gccaccacac
    22681 gcccctgctg cgttccgacc agaacgccgc aaccagtacc gcagaaacgg cacggcgctt
    22741 tatcccattt aatggcttcc tgctgaccaa caacggcgcg ggcaacgccc ggcacgctga
    22801 gaccggcagc cgccgcagcg gccgcaacgg cgttagcttt cataaagcta cgacgactga
    22861 gtttcatggt gtttcctcac cttgctcttc ctgctggtga taaaccagcg acaccgccag
    22921 cacgccctct acgttgcgta ctgactcaat ggtttggatc agcgtttcgc tgtcttctgc
    22981 ttccaccacc acaatcaact gaccgctcgg cgcgtcgctg acagcaactt cacagccggg
    23041 aaaggcgttc agttgggtgc tgatgtctga aattcgttcg cttttggcct gcacaaccag
    23101 gctgcaaact tgccagttag tgtgcatgga gatactccgc agttatggct gataccggac
    23161 agctggctgc acatgctcca catccgttgc aaagttggct attaagttgc ggctggtaga
    23221 tcccggagag cgtcgggcga aagataattg ccattggctc acagctatcc tgacagcggc
    23281 ggcattcaac tgactgatac gccaggcacg cgtccccaat ggtgaactgc aaatcccagg
    23341 ccctggtgtg gcgcggagaa aatagcgatt cggggcaagc ctgcgcgcag gcgtaacaga
    23401 agctgcactc gttatttttg aaattaacgc tcggatagcc gcccgcgccg cgttgcagaa
    23461 tgttgttttc acaagcatta atacaggcgt cgcagcgggt gcaatgggtc agaaaatgag
    23521 attcatcacc ggaccagggc ggacggatac cgttactggc tttgcgccag cgaccagtga
    23581 gtatgccccg acgggatgca tcaatcttca cattgacctt ccatcattaa cgcgctctga
    23641 aaattgagag cgaccaaata aaccgcataa ttaataagcc atttttatag ccgctaagat
    23701 attaaaggat gtgtcaaaga tgcatacccc gatcgaggta aaacctgtag caggatcaaa
    23761 agagtggcgg gaagcgtggc aaaaacgggc ttttgctcac atttcaaatg gttataaata
    23821 tatttatata gcgattaatt caccagagat atttctgctg gtttgctctc tcatcagaat
    23881 ttaacactaa aagagcaggt aaaattgtct gaatattctt taagttattc ataaagcaaa
    23941 ttaataaatc tgatgaatat gttaaccttc agcgacatca tcggtgcaaa cctataaatg
    24001 aagaaggaaa gcaaaaaatg aagaccattc tacctgcagt attgtttgcc gctttcgcca
    24061 ccacttccgc ctgggcggca gagagcgtcc agccactgga aaaaatcgcg ccttatccac
    24121 aagctgaaaa aggcatgaag cgtcaggtga ttcagttaac cccgcaagaa gatgaatcta
    24181 ccctgaaagt agaactgtta atcggtcaga cgctggaagt cgattgcaat ttgcatcgtc
    24241 tcggcgggaa gctggaaagc aaaacgctgg aaggctgggg ctatgattat tatgtctttg
    24301 ataaagttag ctcgccggtt tccacgatga tggcctgccc ggatggcaag aaagagaaga
    24361 aatttgtcac cgcgtatctg ggcgatactg gaatgctgcg ttacaacagc aagctgccga
    24421 tcgtggtgta tacgccagac aatgtagatg tgaagtaccg cgtctggaag gcggaagaga
    24481 aaattgacaa cgcggtagtt cgctaaactg ccgtgaagtg cggcaccccg taggttggat
    24541 aaagcggtca cgccgcatcc gacatctaac gcccgagccg gttgcctgat gcgacgctgg
    24601 cgcgtcttat cagcatacgc cacatccggc ataccatgcc ggatgtggca catcattaca
    24661 acgcaatatc cgccacttct ttttgaacgg gttgcggttt caactgcggt ttcggcgtac
    24721 tatctgctgc ttgcggtttg tcgtatttca gccccagcac ttcgctggtg tactgcaact
    24781 cgcgttctgt tgccgctaca tcaccgttca gcttgcgtcc ataagatgga acgatcgctt
    24841 tcaacgtagc ctgccattgc gggctggaaa cgcgatcgcc aaatactttt tccagcagat
    24901 tcaacataat cggcgcggcg gttgacgccc ctggcgatgc ccccaggagc gcggcaatgg
    24961 ttccttgctg gtcactgacc acttcagtac ccagacgcag tacaccacct ttatcggcat
    25021 cacgcttgat aatctgcaca cgctgccccg cttgccacaa acgccagtcc tcttttttcg
    25081 cttgcggata gtactctttc aacgcttcaa aacgatcctc ttcactcaac atcacctgac
    25141 tcaccagata tttcaccaga tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt
    25201 tagaggtggt ggtggaactc atcagatccc acaatgaacc gtttttgagg aatttggttg
    25261 agaaggtggc aaatggccca aacagcacta cgcgtttacc gtccagaacg cgggtatcga
    25321 tatgcggaac cgacatcggt ggtgcgccaa cggatgcttt accgtaaacc ttcgccagat
    25381 ggtgattaac cacgtccggg ttttccgaaa caaggaactg tccgcccacc gggaaaccgg
    25441 cgtagtcttt cgcttccgga atccccgatt cctgtaacag cttcagtgcc gcaccgcccg
    25501 cgccgataaa gacaaatttc gcacggatgt tctgtgcagt gccatttttc agatcggcaa
    25561 cggtaacggt ccaggtgtta tcgtcattac gctttagggc gcggacttcg ctgctgagtt
    25621 gcagcgagaa gttagatttc ttctgcaagg aagcaattaa ctggcgggtg atctcgccgt
    25681 agttcacatc ggtaccaatt tccgtacgcg tggctgccac tttctgttgc ggatcgcgcc
    25741 cttccatcac taacggtgcc cactctttga tctgcgcgtg atcttcagag taacgcatac
    25801 cgcgaaacag cgagctttgt tgcaacgcgg cgtaacgggc gcgcaggaaa ttgacgttat
    25861 cctcgcccca gacaaagctc atatgcggaa cggtattgat aaatgaacgc ggagtacgca
    25921 gcacgccgcg ctcgacctgg tgcgcccaga actggcggga aatctgaaat gcttcgttaa
    25981 tggcgactgc tttttcaata ctgatgctgc catcggcgtt ttgcggggtg tagttcagtt
    26041 ccatcagtgc agaatgcccg gttccggcgt tattccagcc gttcgaactc tcctgcgcga
    26101 caccctccag gcgctccacc atggtcatcg accattcagg ctccagctcg cgtaaatagg
    26161 tccccaacgt ggcgctcata atgccgccgc caatcaacag tacatcagtt tcctgctcct
    26221 cggacgcttt cgcttttgcc gccatcgaaa cggcattaag ccccacggcc atcgagaaga
    26281 gcatggcagt cacttttttc atcttgttaa tgccttactt ttagtcgctt tattgcaggt
    26341 gagatttgcg cggcatcaac ggtaacact
//
LOCUS       DAZKFZ010000052        26068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000052 DAZKFZ010000000
VERSION     DAZKFZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHOQW_001935"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHOQW_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP9573019.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHOQW_001936"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHOQW_001936"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP9573020.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="ACHOQW_001937"
     CDS             complement(938..1405)
                     /locus_tag="ACHOQW_001937"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9573021.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHOQW_001938"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHOQW_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9573022.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHOQW_001939"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHOQW_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9573023.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHOQW_001940"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHOQW_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9573024.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHOQW_001941"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHOQW_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9573025.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHOQW_001942"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHOQW_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9573026.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHOQW_001943"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHOQW_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9573027.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHOQW_001944"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHOQW_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9573028.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHOQW_001945"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHOQW_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9573029.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHOQW_001946"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHOQW_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9573030.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHOQW_001947"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHOQW_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9573031.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHOQW_001948"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHOQW_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9573032.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHOQW_001949"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHOQW_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9573033.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHOQW_001950"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHOQW_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9573034.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHOQW_001951"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHOQW_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9573035.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHOQW_001952"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHOQW_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9573036.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHOQW_001953"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHOQW_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9573037.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHOQW_001954"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHOQW_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9573038.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHOQW_001955"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHOQW_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9573039.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHOQW_001956"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHOQW_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9573040.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHOQW_001957"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHOQW_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9573041.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHOQW_001958"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHOQW_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9573042.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHOQW_001959"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHOQW_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9573043.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHOQW_001960"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHOQW_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9573044.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHOQW_001961"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHOQW_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9573045.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHOQW_001962"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHOQW_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9573046.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHOQW_001963"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHOQW_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9573047.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHOQW_001964"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHOQW_001964"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9573048.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHOQW_001965"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHOQW_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9573049.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHOQW_001966"
     CDS             15545..15913
                     /locus_tag="ACHOQW_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP9573050.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHOQW_001967"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHOQW_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP9573051.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHOQW_001968"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHOQW_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP9573052.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHOQW_001969"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHOQW_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9573053.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHOQW_001970"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHOQW_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP9573054.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHOQW_001971"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHOQW_001971"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9573055.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHOQW_001972"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHOQW_001972"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9573056.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHOQW_001973"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHOQW_001973"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9573057.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHOQW_001974"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHOQW_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP9573058.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHOQW_001975"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHOQW_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP9573059.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHOQW_001976"
     CDS             23092..23634
                     /locus_tag="ACHOQW_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP9573060.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHOQW_001977"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHOQW_001977"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP9573061.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHOQW_001978"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHOQW_001978"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9573062.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHOQW_001979"
     CDS             25031..25288
                     /locus_tag="ACHOQW_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP9573063.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHOQW_001980"
     CDS             complement(25264..25818)
                     /locus_tag="ACHOQW_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9573064.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6689 a         6177 c         6762 g         6440 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgtttcgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtatagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacgcg cagcgttggc tggtgctgtt aacaccctca
    24481 agcggttaga agatggacgc ttgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgcaggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgagcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcctactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttacaggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacttgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctg
//
LOCUS       DAZKFZ010000053        25785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000053 DAZKFZ010000000
VERSION     DAZKFZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(161..832)
                     /gene="queE"
                     /locus_tag="ACHOQW_001981"
     CDS             complement(161..832)
                     /gene="queE"
                     /locus_tag="ACHOQW_001981"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP9573065.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            987..1607
                     /locus_tag="ACHOQW_001982"
     CDS             987..1607
                     /locus_tag="ACHOQW_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFP9573066.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1621..2295
                     /locus_tag="ACHOQW_001983"
     CDS             1621..2295
                     /locus_tag="ACHOQW_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9573067.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2529..3677
                     /locus_tag="ACHOQW_001984"
     CDS             2529..3677
                     /locus_tag="ACHOQW_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9573068.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            3674..4588
                     /locus_tag="ACHOQW_001985"
     CDS             3674..4588
                     /locus_tag="ACHOQW_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9573069.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4648..5946)
                     /gene="eno"
                     /locus_tag="ACHOQW_001986"
     CDS             complement(4648..5946)
                     /gene="eno"
                     /locus_tag="ACHOQW_001986"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9573070.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6034..7671)
                     /gene="pyrG"
                     /locus_tag="ACHOQW_001987"
     CDS             complement(6034..7671)
                     /gene="pyrG"
                     /locus_tag="ACHOQW_001987"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP9573071.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(7899..8690)
                     /gene="mazG"
                     /locus_tag="ACHOQW_001988"
     CDS             complement(7899..8690)
                     /gene="mazG"
                     /locus_tag="ACHOQW_001988"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP9573072.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8761..9096)
                     /gene="mazF"
                     /locus_tag="ACHOQW_001989"
     CDS             complement(8761..9096)
                     /gene="mazF"
                     /locus_tag="ACHOQW_001989"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP9573073.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9096..9344)
                     /gene="mazE"
                     /locus_tag="ACHOQW_001990"
     CDS             complement(9096..9344)
                     /gene="mazE"
                     /locus_tag="ACHOQW_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP9573074.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9422..11656)
                     /gene="relA"
                     /locus_tag="ACHOQW_001991"
     CDS             complement(9422..11656)
                     /gene="relA"
                     /locus_tag="ACHOQW_001991"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP9573075.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11704..13005)
                     /gene="rlmD"
                     /locus_tag="ACHOQW_001992"
     CDS             complement(11704..13005)
                     /gene="rlmD"
                     /locus_tag="ACHOQW_001992"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9573076.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13062..15818
                     /gene="barA"
                     /locus_tag="ACHOQW_001993"
     CDS             13062..15818
                     /gene="barA"
                     /locus_tag="ACHOQW_001993"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP9573077.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGCGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(16051..17391)
                     /gene="gudD"
                     /locus_tag="ACHOQW_001994"
     CDS             complement(16051..17391)
                     /gene="gudD"
                     /locus_tag="ACHOQW_001994"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP9573078.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLLTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17412..18752)
                     /gene="gudX"
                     /locus_tag="ACHOQW_001995"
                     /pseudo
     CDS             complement(17412..18752)
                     /gene="gudX"
                     /locus_tag="ACHOQW_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(18754..20106)
                     /gene="gudP"
                     /locus_tag="ACHOQW_001996"
     CDS             complement(18754..20106)
                     /gene="gudP"
                     /locus_tag="ACHOQW_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP9573079.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(20541..20990)
                     /gene="yqcA"
                     /locus_tag="ACHOQW_001997"
     CDS             complement(20541..20990)
                     /gene="yqcA"
                     /locus_tag="ACHOQW_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9573080.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(21008..21790)
                     /gene="truC"
                     /locus_tag="ACHOQW_001998"
     CDS             complement(21008..21790)
                     /gene="truC"
                     /locus_tag="ACHOQW_001998"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP9573081.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(21790..22119)
                     /gene="yqcC"
                     /locus_tag="ACHOQW_001999"
     CDS             complement(21790..22119)
                     /gene="yqcC"
                     /locus_tag="ACHOQW_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP9573082.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(22741..23286)
                     /gene="syd"
                     /locus_tag="ACHOQW_002000"
     CDS             complement(22741..23286)
                     /gene="syd"
                     /locus_tag="ACHOQW_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP9573083.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            23354..24202
                     /gene="queF"
                     /locus_tag="ACHOQW_002001"
     CDS             23354..24202
                     /gene="queF"
                     /locus_tag="ACHOQW_002001"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP9573084.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24314..25678
                     /gene="ppnN"
                     /locus_tag="ACHOQW_002002"
     CDS             24314..25678
                     /gene="ppnN"
                     /locus_tag="ACHOQW_002002"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP9573085.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6377 a         6735 c         6169 g         6504 t
ORIGIN      
        1 ttttcactcc agcgaaaaat taacgccaca gaatttgtag agaatgataa tgattagtcc
       61 gggataggca agatggatga gttttcagag acgacaaaga atataaatga gtagaagtct
      121 ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata tttaaatatt
      181 tatgggtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg cacaaacgtg
      241 tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta tcatcggtca
      301 gcgtcgccaa aagttcatcc agtgcttcaa tatcacgtac gcgtcccact ggatgcttga
      361 tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc atgttcagct
      421 ttggcgatac ggtaacccag gtattcggtg tacagcgtac ttcatgagta ccactggttt
      481 cgatctggca gctaaaaccg ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa
      541 tgcaaggctc accacccgta atcaccacat gccgcgcggt atatccctgg cgaccaatga
      601 cagccagcaa gtcttcactg ctcgccgccc cccacttatc actctctttg gtcttcgcca
      661 gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc
      721 accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg acgccggtaa
      781 agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc atagcattct
      841 ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat gtcttattaa
      901 cattctgtta caggcaggtt tatggcaaaa agtggattag atcataaaga ataaaatgaa
      961 gcagagtatt atgttttaag gaattaatta aaagggattt aaacacgctc agaatattca
     1021 tcgtaattat atttattttc aatttatcag gctgtggata taacgatatt caaacctatg
     1081 atgagcaggt taacgcctcc tggtctgagg tattgaatca atatcaacgt cgcgccgatc
     1141 ttattcctaa tcttgtcgcc agcattaaag gttattccag ccatgaacga gatgtactgg
     1201 aagccgtaac attggctcgt agccaggcta atcgcgccag tagcgatctt cagcaaactc
     1261 cgggagatga acaaaaatta caagcctggc aacaggctca agcgcaggtg actcgcactc
     1321 tcggtcagtt aacgataatt agtgagcgtt accctgagtt aaaatctcag gaactctatc
     1381 aaaatttaat ggtacagctt gaaggaagtg aaaatcggat tgccgttgcg cgaggaagat
     1441 atattaaagc cattgaacaa tataatgtca ctatccgcaa attccccgct gtattaaccg
     1501 caaaggtcat ggattacaca ccaaagaaaa attatttgcc ggatgatgtc gccgcagtaa
     1561 gtaaagcccc aacaatagat tttagccaga atgctaatgc tcattaagcg gggaagttct
     1621 atgcgaaatt ttatcttcct tatggctttc ttctgttcat ctgtgtttgc cacacaaatt
     1681 ccagtacctg aatcacccaa gtatgtgaat gacttaaccg gcacattaac aaacagcgaa
     1741 gtaaacaccc tgaccaacca gattaaagcc ctgacgcaaa aaaactacgc acagttggtc
     1801 gtgttagtgg ttgaaacaac aggcgatgaa accatcgagc agtacgcgac gcgggtattc
     1861 gatagctgga aaccgggtga taaagaccgc gatgatggcg ttctactgtt agtcgcctgg
     1921 caggatcata ccgtgcgtat tgaaatcggt tacggactgg aaggtattat tacggatgcg
     1981 cagtccggga aaattattcg caacagtatt attcctgcat ttaaaaaagg cgacctggct
     2041 ggtggtttgc aaaagggaat cagcgatatt gaaagtcgtc tgacggggaa taatttagca
     2101 acgataacac cgaccgatca ccctttgccc tttagcggtt ggtgggcatt gttagtctgg
     2161 gcgatagtac tgacgtttat ttctgccaga ggatatatca aaacgctggg agtaatttgc
     2221 ttcgcagcta ttgtactggc ctttgtttta cctatagcag gcttcagcgg aagctgggga
     2281 gtactcgcaa catgactctg cttcgccaca ccgttcctgg ctgtcgccat cgcttttacc
     2341 cccttcggca aaaaagttcg ggatttgatg cgtgatgcca accagccatc acgccataca
     2401 agaagcaatt cttcctccca ctattcatca tcgtcatcct cttcattcga caatgacaat
     2461 tttagcggtg gtggcggttc atctggtggc ggtggcgctt ccgggcgctg gtaacaaaag
     2521 gaattgatat gcgtattttt ctcttacttc tgatgctatg cggttttcag gtattcgctg
     2581 aacgattgcc ttcagtgact gacttaaccg ggacattaac gacagaagaa caaactgctt
     2641 taactcaaca attacagact ctcgagcagc aaaaccactt ccaggtggcc gtgttggtta
     2701 cgccgaccac agggggcaaa aacattaaac ttgcagcctc gcagagatac ggggtatatc
     2761 actggaaacc cgttggagag aagcgatatg gcgaaggtat cttaattctc gtcgtatggc
     2821 cggaagggct ggcgtcaatg aagattggac atggactgga agagatgttg ccgccggaac
     2881 aagctgcgca aattgttcgt taccatatgc agccagaatt cgaaaaaaac aatctctttg
     2941 ctggattaac gggcggaatt gaaagcatcg cacagtttac ccatatcaaa gcgaacctta
     3001 gccctttgga tgctttagcc aatcatctgt ttgctaatcc gcaacgttcg ctgccatgtc
     3061 ttgcctggac actgctgatg atcgttgcca tcgttgtcct ctggctattt gccagccgcc
     3121 cacggcgtgg catatggatg atatcgatga ttacccctat ggtctggttt ttctcctttc
     3181 aggacgatgt catcattcgg cgggtatcgg tcgtttgttt tagtttattg ttagcaacaa
     3241 cctgcaagca gcgtctcgcg gtagttttta atatgtgccg cgtatttcct atgatgctga
     3301 ccccaaaaaa taaaaacgct aaagtgaaaa aacaaaagaa gactccgcag gttcgtgaat
     3361 caggacgaaa ttcattgttt gtcgtgatgc tgataattat tgtgggatgg tgcctggtgt
     3421 tcgccagtaa taaagatatt gcattcatct caacaattat tgggttaatt gatcatttta
     3481 tcggcccaat aaccattgct ggcatcgttc tgctgatcat tgtggccaaa tttactggca
     3541 atcttaagct ggggtcagga gaaaaaagca acaggaaaac ggggaatcgt aatgctcatt
     3601 ctcgctcttc gtcgagaaat tctttccgcg gtggtggcgg ttcttcagga ggcggcggtt
     3661 catccgtccg ttggtgatcg taatgcgctt aatctttatt ctattcactt tgtggtgcct
     3721 tcctgggctt gcccagcaaa ttgctgtgcc ggaactgcgc caacaggtga ccgatattac
     3781 tggtacgtta agcacttctg aacaacaatc actgacacag caactgcagg atattacaca
     3841 taaaacacgg gcgcaggtcg ctgtattagt agtgccgtcc accggagacg attccattga
     3901 acaatatgct acaagggttt ttgatagctg gaaactggga gataagcaga gaaacgatgg
     3961 tattttactt ctggttgcct gggaggatca tgctgttcgt attgaggttg gctacgggct
     4021 ggaaggggtg gttaccgacc tgcaagcggc gaagattatc agggatatat tgatccctgc
     4081 cttcaaaagt gatgacctga tgggtggatt aacactggca agcgaaaata tcggcgcgct
     4141 tctgttgaat ggtgaattac cggaagacag gggggattat tacagtatca atccccctat
     4201 tccattatcg cttgctgtaa ttatattgct ggcggtactt tcatatttta ttgtttttac
     4261 cgatccgtcg aatttaccct ggatcactct cactggcgcc atttacggga tggtattcct
     4321 ctatgttgcc gagccaggac catggaccaa tttaatcgtt gcctgcggta tgttaacgcc
     4381 ttttgcgatc gtaccactgg ttattttttg gcttatcgtc aataaaaagc tacgcgccaa
     4441 atacaaaaaa ctcagtaaag atagagcttc aagaaaaggt tcttcttcat cctcttccgg
     4501 tggcggctca agtggcggag gctcaggcgg cggatttagc ggcgggggcg gttcttctgg
     4561 cggtggcggc gcgtccggtc gctggtaact caaccataaa aaacgccagc ccgaaggctg
     4621 gcatttttaa atcagataaa gtcagactta tgcctggcct ttgatctctt tacgaccgtt
     4681 gtacggtgct ttttcgccca gagcttcttc gatacgaatc agctggttgt atttagcaac
     4741 gcggtcagaa cggctcatag aaccagtttt gatctggcct gcagcagtac caacagccag
     4801 gtcagcgatg gtagcgtctt cagtttcgcc agaacggtga gagataactg cagtgtagcc
     4861 agcgtctttc gccatcttga ttgcagccag agtttcggtc agagaaccga tctggttgaa
     4921 tttgatcagg atggagttag cgataccttt ttcgatacct tctttcagga tcttggtgtt
     4981 ggttacgaac aggtcgtcac caaccagctg gattttgtcg cccagaactt tggtctggta
     5041 tgcgaaacca tcccagtcag attcgtccag accgtcttcg atggaaacga tcgggtactg
     5101 tttggtcagt tcttccagga agtgagtgaa ttcttcagag gtgaatgctt tgttgccttc
     5161 gccagccaga acgtatttac cgtctttgta gaattcagaa gctgcgcagt ccatcgccag
     5221 agtgatgtct ttgcccagtt cataaccagc agctttaaca gcttcagcga taacagccag
     5281 agcttcagcg ttggaaccca ggttcggcgc atagccacct tcgtcaccaa cagcagtgtt
     5341 catgcctttc gctttcagaa cttttgccag gtgatggaaa acttcagaac ccatgcggat
     5401 ggcttctttc acagttttcg cgccaaccgg ctgaatcatg aattcctgga tatcaacgtt
     5461 gttgtcagcg tgctcaccac cgttgatgat gttcatcatc ggaaccggca tggagtattt
     5521 gcccggagtg ccgttcagtt cagcgatgtg ctcgtacagc ggcatacctt tagcagctgc
     5581 agcagctttg gcgttagcca gagatacagc caggattgcg ttcgcgccga atttggattt
     5641 gttttcggtg ccgtccaggt cgatcatgat cttgtcaatg ccagcctgat ctttagcatc
     5701 tttgccaatc agcgcctgag cgatcgggcc gtttaccgca gcaacagctt tggttacgcc
     5761 tttacccagg aaacgggatt tgtcgccatc gcgcagttcc agagcttcac gggaaccagt
     5821 agaagcacct gacggagcag ctgccatacc gacgaaacca ccctccagat gtacttcggc
     5881 ttcaacagtc gggttaccac gggagtcgat gatttcacga ccgatgattt ttacgatttt
     5941 ggacattagg ttttcctcaa gtcactagtt aaactgaaac tccagacaaa caacgcgtac
     6001 ccagggtacg cgttgccgtt ctaacttttt tacttacttc gcctgacgtt tctggaactc
     6061 gctggcggct ttcacaaagc ctgcaaacag cgggtgacca tcacgtggag tagaagtaaa
     6121 ctccggatgg aactggcaag ccacgaacca cgggtgattc ggaacttcga tgatctcgac
     6181 caactgatca tccccggaac ggcccgcaac gcgcagacct gcatcttcaa tctgtttcaa
     6241 cagcatgttg ttgacttcgt aacggtgacg atgacgctca acaattgtcg gcgcattgta
     6301 cagctggcga accaggctat cgtcaaccaa ctggcactgc tgtgcgccga gacgcatggt
     6361 accgccgaga tcgctcttct cgctacgaac ttcaacgttg ccgttttcat cgcgccactc
     6421 ggtaatcagc gccacaaccg ggtacttaca gtctggcaca aattccgtag agttggcgtt
     6481 ctccatgttg gcaacatggc gagcgtaatc aattaacgcc acctgcatac ccaggcaaat
     6541 gcccagataa ggaatattgt tctcacgcgc aaaacgcgcg gtcgtaatca tgccttctac
     6601 gccacgatag ccgaaaccgc caggtacgag gattgcatcc agacctttaa ggatttcaac
     6661 gccgcgcgtt tcaacatctt gtgaatcgat cagtttgatg ttgacgctga cacgattctt
     6721 cagcccaccg tgtttcagtg cttcaatcac ggatttgtaa gcgtccggca gttcaatgta
     6781 cttgccgacc ataccaatgg tgacttcact taccggattc gcttcttcga agataacctg
     6841 ttcccattcg gacagatttg cttccgggca gtttaagctg aatcgtttac aaatataatc
     6901 gtccagcccc tgagatttca acaggcccgg aattttatag atggaatcga cgtctttcag
     6961 agaaataacc gctttttccg gaacattaca gaacaatgca atcttcgcac gttcgttcgc
     7021 cggaacggcg cgatctgaac gacaaatcag gatgtcaggc tggataccga tggagagcag
     7081 ctcttttaca gagtgctgag tcggtttggt tttgacttca ccagacgctg ccatgtacgg
     7141 caccagcgtc aggtgcataa acagagtgtg ctcacggcca atttcaacag ccatctggcg
     7201 aatcgcttcg aggaacggca gggattcgat atcacctact gtaccgccga tttcgaccag
     7261 tactacgtca tgaccttcgc caccttccag cacgcgctct ttgattgcgt tagtgatgtg
     7321 cggaataacc tgtacggttg cgccgaggta gtcaccgcgg cgttctttac gcagaacgtc
     7381 agagtagata cgacccgtgg tgaagttgtt gcggcggctc attttggtac ggatgaaacg
     7441 ctcgtagtgc cccaggtcca ggtcggtttc agcgccgtct tcagtaacga acacttcccc
     7501 gtgttggatt gggctcatag tacctggatc gacgttgatg tacggatcca gtttcatgat
     7561 ggtcacattg aggccacggg cttcaagaat ggctgcgagg gaggctgcgg caatgccttt
     7621 acccagagag gatacgaccc cgccggtcac aaaaatatag ttcgttgtca tgctgaacct
     7681 gagaagttag gttgaaaaga cgatggaata accaggacgg gaaagcagta tacccgaaca
     7741 tgacctgtgc cacaaacttt cattatccct cctcttcgcc agcgcactat tgaaatcagg
     7801 agtgagaaaa tagccccttt ggggtaaatg tttttgacgc aaatcaagcg cttgtcattt
     7861 aaaaaatgac acaaatggcg cttgaccgcg taattccctt agagatcaat ttcctgccgt
     7921 tttacctgtt gccagacttc ttccattgtt tcgaggtcaa cacctgtcat ttccagtcca
     7981 cgcgcggcaa caatacgctc cacttcgcga aaacgacgct cgaatttttc gttcgctttt
     8041 tgcaatgcga tttccgcttt tgtccctaaa tgacgagcca gattgaccgt ggcaaacagc
     8101 aggtccccca tttcctcctc cagtttagcc tggtcgacaa cagcctgccg cgcttcgtac
     8161 atcacctcgt cgatctcttc gtagacttta tcgactaccg gaccaagcgt cgtccaatcg
     8221 aagccaacgt tggcgcaacg tttctggatt ttttgcgcac gcattaaagc cggtaaacta
     8281 cgaggaatat cgtccagcgc cgaatgctgc gctttctgcg cgcgctcttc ggttttgatt
     8341 tgctcccaac gggcaagcac ttcactactg ttttcggcag aactgtcagc aaaaacatgc
     8401 ggatggcgac gctctaattt atcgctaatc gcagcgcaaa tatcattaaa gtcaaagcgc
     8461 ccttcttcct gagccatttg ggcgtaaaac accacctgga acagcagatc gccgagttca
     8521 ccgcgcagat cgtcaaaatc ttcacgggcg atggcgtcca gcacttcgta ggtttcttca
     8581 agggtgtaag gcgcaatggt ggcaaatgtc tgctctttat cccacgggca gccgttttcc
     8641 gggtcgcgca ggcgctgcat aatagtgagc aaacggtcga tttgattcat tagaatgtcc
     8701 tgaaaattgc gggtccgtca ggtggaaacc tgtgaccaga atagaagtga gttagtaaca
     8761 ctacccaatc agtacgttaa ttttggcttt aatgagttgt aattcctctg gggcaaccgt
     8821 tcctttcttc gttgctcctc ttgcccgcca ggcgatactt tttacctgat cagctaacgc
     8881 tacgccatca cgttcctgac cggataaaac aacttcgaac ggatatcctt ttgattgcgt
     8941 tgtacaagga acacacagac acatacctgt tttgttgttg tacatgaacg gactcaggac
     9001 aacagccgga cgatgtccgg cttgctcgct accttttgtc gggtcaaaat caacccaaat
     9061 cagatcgccc atatcgggta cgtatcggct taccattacc agacttcctt atctttcggc
     9121 tctccccagt cgatattctc gtggaggttt tccggcgtga tgtcgttgac cagttcagca
     9181 agcgtaaata cgggctcttt acgcactggc tcaataatta atttgccatc caccaggtca
     9241 atcttcactt catcatcaat attcagattg agcgcctgca ttaacgtagc cgggatccgc
     9301 accgccggtg aatttcccca acgctttacg ctactgtgga tcataaccct ttcctcaaac
     9361 cactatcata tgtagataca gtatatatca atctacattg tagatacgag caaatttcgg
     9421 cctaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg agtttaccca
     9481 gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg gtcgccagtt
     9541 gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg ttggcgagaa
     9601 tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg accaccagcg
     9661 aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct ggcgcatggg
     9721 agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg cgtccctggg
     9781 taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg atgtggtgca
     9841 tcaggttacc aacaccttca accactacac gaccgttatc tttactgcgg ttttgcggcg
     9901 tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg gcactcggct
     9961 tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc ccgccaccaa
    10021 tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga tgtttttctg
    10081 cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc cgcccagcca
    10141 gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa cgcccacggc
    10201 tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc ggctgtttct
    10261 gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc acaatgcgcc
    10321 cgccaatttt tgccccgata cagcggtgtc cgacatcact gtggatgtgg taagcgaagt
    10381 ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttaggcgta aagacgtaca
    10441 cccggtcgtc aaagacctgg ctgcgtactt cgtcgagcat ttcgccggaa tcagccatct
    10501 cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt cccgaacgtg
    10561 cgccgccagc agccgcgccc tctttatatt tccagtgcgc agcaacaccc aactctgcat
    10621 cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc ggccccagaa
    10681 ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa tcgtcaaact
    10741 catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa caatcctgta
    10801 aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag gcgaggttct
    10861 ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac acttcagctt
    10921 taacgccttc agctttcatc tcagcgcgca gatgaccgac aaactcttcg atatagtgtt
    10981 cgcggtcgag acggcgttca tgcaacagtt ttgcgatgcg tttgtattcg gtcgggtgga
    11041 gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca agacggtttg
    11101 ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacgcgttca tcttccggcg
    11161 catcttttac ttcacgcaga tgagcaatac gctccgccag tttgatgacc acgcagcgaa
    11221 aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg gaggaaacag
    11281 aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg ccgtgaataa
    11341 ggttaacgac cgacttaccg acgctctcac gcagcacatc ttcgctgact acgttggcat
    11401 cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt aatgtcgaga
    11461 ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga tgcccctgcg
    11521 tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc tggctggtaa
    11581 tacccagact tgcgatccat ttttccggat caaattcacc agccttattg atatgtgcac
    11641 ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat cggcaaatcg
    11701 caactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt gtgggaacat
    11761 atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt cgctatcccg
    11821 agccagcgtt gcagggttac aggatacata aactatacga ataggttcca gttttataat
    11881 ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt tatcgaagcc
    11941 gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat aaaacgtcac
    12001 attctgtaag cagttaagac gcgcattctg ctggcctttt tccaccagcg ccggaacacc
    12061 ttctacaccc accacactgg cagcttgtgt cgccaatggt agtgtaaagt tgcccatacc
    12121 gcagaacaga tccagtacgc gatcttcagg ttccacctcc agccattcca acgcacgcgc
    12181 taccattttt tggttcacac ccgcattaac ctgaataaaa tcgcgcgggc taaaagttaa
    12241 gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga gtatctcact
    12301 atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca gtttttcgcg
    12361 atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc tggttgcctg
    12421 taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccaggc atgccctgac
    12481 tttgggcagc aatgcttcaa gttggggcac taaaatggga cattgtttaa cgtcgacaat
    12541 gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg gtaagtagtt
    12601 cagacttaaa cgcgcgcgac ggcgatagcc ccagggaaca tcggcgatca cttcagagac
    12661 ttcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta aatccacgct
    12721 ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg gcgtttcgcg
    12781 ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt ttttatcctc
    12841 agtaacagta acttccgcgt tttcctgcgg caataatccg gggataaata gcgttttgcc
    12901 gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa cggttatgat
    12961 ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg agactttctc
    13021 aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac tacagcctgc
    13081 gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta ttgctgagta
    13141 tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat gccggtgcca
    13201 gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa aatcgcgaat
    13261 ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt cgcgcgattt
    13321 cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt gatccctcat
    13381 caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact cgtgacggcg
    13441 atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac gaatcgccca
    13501 gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag ctggatctta
    13561 aatcggttcg cttgcagcaa tataaagaga tctttatttc cagcgtgatg atgctgtttt
    13621 gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc ggtccgattc
    13681 gcaacatggt gaatactgtc gaccgcatcc gtcgcgggca actcgacagc cgagtggaag
    13741 gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg gcaatgtcgc
    13801 tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc gatctgcgtg
    13861 aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa aagcgcgccc
    13921 aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag ctgcgtacac
    13981 cactgaatgg tgttattggt tttacccgcc tgacgctgaa aacagaatta acaccaacgc
    14041 agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca attattaatg
    14101 atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt attccattcc
    14161 cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct cacgataaag
    14221 ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc ggcgacccac
    14281 tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc accgagaatg
    14341 gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg cagattgaag
    14401 tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc ttattccagg
    14461 ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg ctggggctgg
    14521 tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat agccagccga
    14581 atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac attattattg
    14641 aagggccatc cacccagtgc ctcgccggta aacgcctggc ctatgtcgaa ccaaactccg
    14701 cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg gtttatagcc
    14761 caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc atcgcggtga
    14821 ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta tcgatgaccg
    14881 atttcctgat gctggcactt ccttgccatg cacaagtcaa tgctgaaaaa ctcaagcaag
    14941 atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg cctgccctga
    15001 cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa agtaagctgg
    15061 caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc ggcgcattgc
    15121 tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggcg gttgaacggg
    15181 cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgcctgac atggatggca
    15241 ttcgggcctg cgagctcatc caccagctcc cccatcagca acaaacgccg gttatcgcgg
    15301 taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg atgagcgatt
    15361 atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc tacaagcctg
    15421 gctgcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg gtgaacccga
    15481 atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc gatttagcgc
    15541 gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa gttgaggaac
    15601 agctggttgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg catggcagtt
    15661 gcggctatag cggtgtgccg cgtatgaaga atctctgcca acttatcgaa caacagctac
    15721 gtagcggtac taaagaagaa gatttggaac cggagctgct ggaactgttg gacgagatgg
    15781 ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt cgagtagcaa
    15841 tcggcttttt atgacgctgt tttcagattg gtgacaaagt gcctgtgttt atgccggatg
    15901 cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaataaatt aaaaagcatg
    15961 taggcctgat aagcgtagcg catcaggcaa ttttgtgttt ggcatcagtc tcaaaccggc
    16021 tccagataga gccggttttg gttttttgtc ttaacgcacc atgcacgggc gcttgttatc
    16081 gaacgtccag ccaggaatca gatactgcat tcccatcgca tcgtcacgcg cgccaagccc
    16141 gtgtttctga tacagctcat gggctttcat cacttgatcc atatcgattt ctacacccag
    16201 ccccggtttt tctggcacct gtaccagccc gcctttgatc tcaaacggtt ctttggtcag
    16261 gcgctgatta ccttcctgcc agatccagtg cgtatcaata gcagtaattt tacccggtgc
    16321 agcggcggca acatgggtaa acatcgccag ggaaatatcg aagtggttgt tagagtgtga
    16381 accccaggtc aggccaaatt catggcacat ttgcgccaca cgtaccgaac cttgcattgt
    16441 ccagaaatgc ggatccgcca gcgggatatc aacggattgt aacgagagcg tatggcccat
    16501 ctgccgccag tcggtggcga tcatattggt tgcagtcagc aggccagtcg cgcgacggaa
    16561 ctctgccatc acttcacgcc cggagaaacc ttgctccgca ccacacggat cttctgcata
    16621 agccagcgaa cctttcaggt atttgccgat tttaatcgct tcgttcagcg accaggcacc
    16681 gttaggatcg agcgtaatac gcgcctgcgg gaagcgttgc gccagtgcca caatagactc
    16741 ggcctcttct tccccggcca gtacaccgcc tttcagtttg aaatcgttga agccatattt
    16801 ttcatacgcc gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt cttcatgacg
    16861 caggcgatac cagtcgcatg agtcatccgg ctggctttga tacggcagag gtgtggcttt
    16921 gcgattaccg acgaagaaca gataaccgag catttcgact tcgctacgct gttgaccatc
    16981 gcccagcagg gatgccacgt taacccccag atgctgcccc agcagatcca gcattgccgc
    17041 ttctatcccg gtaactacat gaatagtggt acgcaaatca aatgtctgca aaccgcgccc
    17101 gccggcatca cgatcggcaa aagtattacg caccagcgtc agaacgtttt tgtattcacc
    17161 cagcgtttta cccactacca gcggaattgc atcttccagc gttttacgga ttttctcgcc
    17221 gccgggaatt tcccctacgc cagtgtgacc agaattatct ttgataatca caatattacg
    17281 cgtgaagaac ggtgcgtgtg caccactcag attcatcagc atactgtcat ggcccgccac
    17341 cgggatgacc tgcatttcag taacaacagg cgtcgtaaat tgagaactca tatttatgtc
    17401 ctttttcaga atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt ccagccgggg
    17461 atcaggtact gcatcgggcc tgcgtcgtta cgcgcaccgc caggcagacg tttataggcc
    17521 tcatgtgcct tttgtacctg ttcccagtcc agttccacgc ccagaccggg cgcatcagga
    17581 acggcaattt ttccgttttt aatctccagc ggattttggg tcaggcgaca atcgccctcc
    17641 tgccaaatcc agtgggtatc gatagcggta ggattacccg gtgccgccgc gcccacatgg
    17701 gtaaacatcg ccagagagat atcgaaatgg ttgttggaat ggcagcccca ggtcagcccc
    17761 cagtcgtcgc aaagctgcgc cactcggact gcaccggaaa gcgtccagaa gtgcggatcg
    17821 gcaagtggaa tatctaccgc attgagcatc accgcatgac ccatttcgcg ccagttggtg
    17881 gcgatcatat tagtcgcgac gggcaagccg gtcgcccgcc gaaactccgc catcacttca
    17941 cgtccggaga agccctgttc tgcgccgcat ggatcttcgg cataggtaag aacatcattc
    18001 agccctttgc acagagaaat ggcttcatca agcagccatg caccgttggg atcaacggta
    18061 atccgcgcat ccgggaagcg tttcttcaat gcacgaacag tgtcgatttc ttgctcgcca
    18121 ggtaacacgc cgcccttaag tttgaaatct ttaaagccgt agcgatcctg tgaggcttcc
    18181 gccagacgca caacggcttc gctgttcatc gctttctgat ggcgcaactg ataccactca
    18241 tggttgcccg gcgtattttc cagataagga agatcggttt tggtccgatc accgatataa
    18301 aacagataac cgaggacggt aatagcatcg cgttgcttgc ctggccctaa cagttcgcag
    18361 accggaacat tcagcgcctt acctagcagg tcaagcaagg cggcttccag cgccgccacg
    18421 gcgttaacgc gcaattcaaa ggtccaggcc cctttaccga aggtatcaaa atcggctgct
    18481 tgattacctt tatgcacctg ctgaaccact ttattcaggc gcgcaacttc ctagcccagc
    18541 accatcggaa tagcatcgac caatgtctga taaatcacct ctccgcctgg cgcttcacca
    18601 atgccggtat gcccggcgtt atcggtgagt accacaatat tgcgagtgaa atatgcgtta
    18661 tgcgccccac caatattaag caacatgctg tcgtacccgg cgaccggaat gactttcata
    18721 tcagtaataa caggactgga ttgtgtcgtc atcttattgc cccacgacag gtttcagctc
    18781 gatacgcttg atatcgccca ccagcaccag gtagctcagt accgcgatta aggcatgaac
    18841 accaacataa atcagcgccc cattaaacga gccagtcgtg ccaacgatat aaccaattgc
    18901 gattggcgtg acgatgccag aaatgttacc gaacatgttg aacaggccac cggaaagacc
    18961 gctgatctct tttggcgcgg tatctgccat tactgcccaa cccagcgccc caatgccctt
    19021 accgaagaag gccagcgcca taaagccgat gatcatccac tcaacgttga cgtagttgca
    19081 gaacaccatc accatcgaca gcaacatgcc cattacgatc ggtgttttac gcgcaatgtt
    19141 cagcgatccc gtgcggcgca tcagccagtc ggaaataatc ccacccagca caccaccgat
    19201 aaaaccacaa accgccggaa cggaagccac aaagcccgct ttcagaatcg acatcccacg
    19261 cgcctgcacc agataaaccg gaaaccaggt aatgaaaaag taagtcaggg cgttgataca
    19321 gtactgaccg atataaacgc cgatcatcat ccgtgacccg agcagttgtt tgatctgccc
    19381 ccacttcacg ctgaacggca ctttaacttt ggtgttttgc tgatccatat tgatcagcgc
    19441 gccacccgcg gcgatgtact ccagctcttt ctgatttacc cccggatgtt gatttggctc
    19501 gtggatgact ttcaaccaga taaagctgat ggcaatgccc agaccgccca taaagaagaa
    19561 gacgtgtgac cagcccactt catgcgtcag ccagcccata atcggcgcga agatcaccgt
    19621 tgcgaagtat tgagcggagt taaaaatcga caccgccgtt cccctttcct gcgccggaaa
    19681 ccaggccgca acaatgcgac tgttgccggg gaaagatggc gcttcagcaa gcccgaccag
    19741 gaagcgcaga gtaaacaggg caacgataat gccgaatcca ctaaagatat cgacgaagcc
    19801 ttgcagcaag gtaaacatcg accagataaa gatcgaccag aagtagacgc gttttgaacc
    19861 aaaacgatcc agcaaccaac caccagggat ctgcccgata acataagccc atgagaaggc
    19921 agagaacaca tagcccattc ccacgggatc aaggccgata tctttggcca tttccgaacc
    19981 ggcaatagaa agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa acaacatcac
    20041 cactatccag taacgagcat tcgtgcgttt ttccacactg ctcgcagcct gacttaaaga
    20101 actcattgtt gcactcctga aaattcgcgt tagccacgct cactctggac tgcgacatcg
    20161 ccaggaaatc agaggtgacg tagggtgttt tttgccgttt ttataggtcg ttcgccgaat
    20221 acggcgcgtg tttatatctg gcaatagcag tataaaaagc gcgccatagc ggctcaccgt
    20281 gcaacaacac aacattaatg cgttcaatga ggcccgattt tggcattagc cctggacggt
    20341 ggaatccact tcacggaaat gaaaacaaga acaagaaagg aagggttaaa acgaagaaat
    20401 aaagaagagt atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta
    20461 tgaagcagtt tttactggca tttgcctgaa aagattcgat tcagcaccgc taaaacgaca
    20521 tttaccgctc gctgaacata tcaggacaac agcgtgcccc actgttcgac ccacggattt
    20581 gattccgttt ccggttccgg gttctcactg gcgtcaatca acagcatttc gccaacccgc
    20641 tgagcgctct gttcctgcag caaggcatcg aactgtttgc cgccattgca gaaattcaca
    20701 taactactgt cgccgagcgc aatcacgcca taacgcagat tcggctggaa gcccagacta
    20761 tctttgattc cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg
    20821 gacgtaacca ccagaacata cttatcctga tagggcagcc agtcgcttaa ttcaggatct
    20881 tcaaataccg ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc ttccgccact
    20941 aacagcgaat ttccgtacat ggtgccgaca aaaataccaa tttccgccat accgttttct
    21001 ccctggatta ggaacttctc tctccatcct gacccgatgg cgcactgaac tcaacccttt
    21061 cattttcagg aagcagaccg cgccagccaa attgtgataa cgcctgcatc caggtgtcgt
    21121 ccaggcccgc gtgaatagtc agcggctcac cagtaaaagg atgcgtcaat gacaactgac
    21181 tggcatgcag cattaaccgc tgaagaccaa aatgctcagc accgctgcga ttctggcgta
    21241 aatcgccatg tttgctatcg ccaataatcg gatgacgcaa atgggcaaga tgtcggcgaa
    21301 gctgatgttt gcgtccggtt ttcggctcca gttccaccag gccgtagcgc gtggtcgggt
    21361 aacgtccggt cgctaccggc atttctacgg tcgccagacc gcgataatgc gtcactgctg
    21421 gctgcgggcc tttatcttcg cgggcaaatt tatcagcgat tttgtccagt tcttccacca
    21481 gtggataatc cagcaccgct tcttccatca accagccgcg cacaatcgca tggtaacgtt
    21541 tctggatttg gtgctgttca aactgttgtg ccagcagccg tccggcctcg ctggataatc
    21601 ccatcaacaa cacaccagaa gtgggtcggt ccagacgatg agcagtaaaa acatgctggc
    21661 ctatctggtc acgcacggtt tgcatgacca ctactttctc gtcgcgatcc agccagctac
    21721 ggtgaaccag ccagccggag ggtttattta ccgcaaccag ccattcatcc tgatagagta
    21781 tttccagcat tagctcgcat catccgcaaa aagagcatcc agtttttcca actcagccag
    21841 aataagcgcg cgttgcggat ggtccgtcgc cagcgccatt tcataatagg gtgcaacggc
    21901 aaaagcgccc ggtaacggct ggttgttatt taacaaatcg tgcattcgcg ggatcagcac
    21961 ccactgcaac cactccagtg gttccatggt gtccataaag aacggttggg tactattaaa
    22021 ttgatgcggc tggggttcat cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag
    22081 cgcctgcaac tggagacgaa cgcggtcatg agtggtcata aagcaacctc aataagaaaa
    22141 actgccgcga aggatagcag gaataaaaaa agggagcact gtattcacag tgctcccggt
    22201 tcgtttcgca gcattccagc tacttttgtt gctccctgct catccttgac aacttttcct
    22261 ctggccatgc ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac caccccgatg
    22321 tgatacttca tcctgaagtg tcccaggcct tcctgaccca ccgaatcatc ctgaccggtt
    22381 ctcattctcc atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc
    22441 cagaagcctt tccctgaaac accatcctgg tgtgtccttc agaagtgtca tcatcctgat
    22501 gttcacttcg ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt cgcgaactct
    22561 tacaaggcgc ttacagacaa tgccttaagg aaaatttcat atgaaagtta ggaattaaca
    22621 agcaacatgt tgtttttaaa tgaaaataac gattcttatc tttttaagta ttcgcttatt
    22681 tcctggcgat ctcctacaga gtctgtaaga gatctctcac aaccagatgg ggtatttgga
    22741 ttacagaaga ggcttaagtt gattaaggaa ttccgcaaga tttggggcta aatgcgtccg
    22801 tttacgtgtt cccagcgtct ctttacacac ttcaccggag agattacaca ccgagatgac
    22861 ctctaactct tcttccagcg tcgcgataaa tagcgtgggt ggcagtttta gccttttctg
    22921 agtcaccaga tgaccaatca gattttcctg cacccgacgg aagtcgtctt cactccaggt
    22981 ttgcagcaat gtcagtttga tatcgccaaa ctgcgcgtgc atatccccgg caaactgagt
    23041 cgtataaaag gtatgaattg tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt
    23101 tacattttgt tccccggtaa acggctgggg ttgccagtat acggcatctt cagtagttga
    23161 aataatgcac ggtgaaggaa cgccgtacag ttcctcactt aacggccagc ttttatgctc
    23221 ttcatgccac gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc
    23281 gtccactaat ttctctcttc acttaaacca gatacacttg tcgtttagtt tatctggttt
    23341 atgacggtga aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc tgactcttgg
    23401 aaaatcaacc gattaccggg atacctatga cgccagccta ctgcaaggcg ttccacgcag
    23461 cctgaatcgc gacccgctgg gtctgaaagc ggataacctg ccttttcaag gtacggatat
    23521 ctggacgctg tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg tcgctgtcgg
    23581 tcatgttgaa cttgattaca ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta
    23641 tctcaacagt tttaaccaga cgcgttttaa taactgggat gaggtgcgcc agacgctgga
    23701 gcgcgactta agcacttgcg ctcagggtga ggttagcgtg gcgttatatc gtcttgatga
    23761 actggaaggc cagccgatag gtcatttcaa tggcacttgc attgatgacc aggatatcac
    23821 tatcgataac tatgaattca ctactgacta tctggagaat gccaccagtg gtgaaaaagt
    23881 agtggaagag acgctcgtca gccacctgct gaaatcaaac tgcctgatca cccatcaacc
    23941 agattggggt tcgatccaaa ttcagtatcg tggacgccaa attgacagag aaaaactgct
    24001 gcgttacctg gtctcattcc gtcatcacaa cgagttccac gaacagtgcg tggaacgcat
    24061 ctttaatgac ctgttacgct tctgccagcc agagaaattg agcgtttacg cacgttatac
    24121 ccgtcgtggc ggtctggaca ttaacccgtg gcgcagtaat agcgattttg tcccatcgac
    24181 cacaagactg gttcggcaat aaattttttc tcaattttgc gtgctggatt cacgcagaag
    24241 gttgtgaaag gtcatcaggc agggctattg taatcaaagg gaatgacgat attcgtccca
    24301 taaggagttt ttcttgatta cacatattag cccgcttggc tccatggata tgttgtcgca
    24361 gctggaagtg gatatgctta aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa
    24421 ctgttcactt gccgtactga actccggtag tttgaccgat aacagcaaag aattgctgtc
    24481 tcgttttgaa aatttcgata ttaacgtctt gcgccgtgaa cgcggcgtaa agctggaact
    24541 gattaatccc ccggaagagg cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa
    24601 cttgttcgcg gttctgcgag acattctctt cgtttacggg caaatccata ataccgttcg
    24661 ttttcccaac ctgaatctcg acaactccgt ccacatcact aacctggtct tttccatctt
    24721 gcgtaacgct cgcgcgctgc atgtgggtga agcgccaaat atggtggtct gctggggcgg
    24781 tcactcaatt aacgaaaatg agtatttgta tgcccgtcgc gtcggaaacc agctgggcct
    24841 gcgtgagctg aatatctgca ccggctgtgg tccgggagcg atggaagcgc cgatgaaagg
    24901 tgctgcggtc ggacacgcgc agcagcgtta caaagacagt cgttttattg gtatgacaga
    24961 gccgtcgatt atcgccgctg aaccgcctaa cccgctggtc aacgaattga tcatcatgcc
    25021 ggatatcgaa aaacgtctgg aagcgtttgt ccgtatcgct cacggcatca ttatcttccc
    25081 tggcggtgtg ggtacggcag aagagttgct gtatttgctg ggaattttaa tgaacccggc
    25141 caacaaagat caggttttac cattgatcct caccggcccg aaagagagcg ccgactactt
    25201 ccgcgtactg gacgagtttg tcgtacatac gctgggcgaa aacgcgcgcc gccattaccg
    25261 cataatcatt gatgacgccg ctgaagtcgc ccgtcagatg aaaaaatcga tgccgctggt
    25321 gaaagaaaat cgccgtgata caggcgatgc ctacagcttt aactggtcaa tgcgcattgc
    25381 gccagatttg caaatgccat ttgagccgtc tcacgagaat atggctaatc tgaagcttta
    25441 cccggatcaa cctgttgaag tgctggctgc cgatctgcgc cgtgcgttct ccggtattgt
    25501 ggcgggtaac gtaaaagaag tcggtattcg cgccattgaa gagtttggtc cttacaaaat
    25561 caacggcgat aaagagatta tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca
    25621 gcatcgtatg aagttgccag gctcagccta catcccttgc tacgaaatct gcacgtaatc
    25681 tcccctcccc cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa
    25741 ctcatttcat ttgttatatg aatgtttctt accaccctca cgcga
//
LOCUS       DAZKFZ010000054        25500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000054 DAZKFZ010000000
VERSION     DAZKFZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            419..1558
                     /gene="wza"
                     /locus_tag="ACHOQW_002003"
     CDS             419..1558
                     /gene="wza"
                     /locus_tag="ACHOQW_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP9573086.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1564..2007
                     /gene="wzb"
                     /locus_tag="ACHOQW_002004"
     CDS             1564..2007
                     /gene="wzb"
                     /locus_tag="ACHOQW_002004"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP9573087.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2010..4172
                     /gene="wzc"
                     /locus_tag="ACHOQW_002005"
     CDS             2010..4172
                     /gene="wzc"
                     /locus_tag="ACHOQW_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP9573088.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4265..5104
                     /gene="wcaA"
                     /locus_tag="ACHOQW_002006"
     CDS             4265..5104
                     /gene="wcaA"
                     /locus_tag="ACHOQW_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP9573089.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5107..5595
                     /gene="wcaB"
                     /locus_tag="ACHOQW_002007"
     CDS             5107..5595
                     /gene="wcaB"
                     /locus_tag="ACHOQW_002007"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP9573090.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5592..6809
                     /gene="wcaC"
                     /locus_tag="ACHOQW_002008"
     CDS             5592..6809
                     /gene="wcaC"
                     /locus_tag="ACHOQW_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP9573091.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6784..8001
                     /gene="wcaD"
                     /locus_tag="ACHOQW_002009"
     CDS             6784..8001
                     /gene="wcaD"
                     /locus_tag="ACHOQW_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP9573092.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8012..8758
                     /gene="wcaE"
                     /locus_tag="ACHOQW_002010"
     CDS             8012..8758
                     /gene="wcaE"
                     /locus_tag="ACHOQW_002010"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP9573093.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8774..9322
                     /gene="wcaF"
                     /locus_tag="ACHOQW_002011"
     CDS             8774..9322
                     /gene="wcaF"
                     /locus_tag="ACHOQW_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP9573094.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9351..10472
                     /gene="gmd"
                     /locus_tag="ACHOQW_002012"
     CDS             9351..10472
                     /gene="gmd"
                     /locus_tag="ACHOQW_002012"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9573095.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10475..11440
                     /gene="fcl"
                     /locus_tag="ACHOQW_002013"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10475..11440
                     /gene="fcl"
                     /locus_tag="ACHOQW_002013"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP9573096.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVEQVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11443..11922
                     /gene="gmm"
                     /locus_tag="ACHOQW_002014"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11443..11922
                     /gene="gmm"
                     /locus_tag="ACHOQW_002014"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9573097.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11919..13142
                     /gene="wcaI"
                     /locus_tag="ACHOQW_002015"
     CDS             11919..13142
                     /gene="wcaI"
                     /locus_tag="ACHOQW_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP9573098.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13145..14581
                     /gene="cpsB"
                     /locus_tag="ACHOQW_002016"
     CDS             13145..14581
                     /gene="cpsB"
                     /locus_tag="ACHOQW_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP9573099.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYEVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14774..16144
                     /gene="cpsG"
                     /locus_tag="ACHOQW_002017"
                     /gene_synonym="manB"
     CDS             14774..16144
                     /gene="cpsG"
                     /locus_tag="ACHOQW_002017"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9573100.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16199..17593
                     /gene="wcaJ"
                     /locus_tag="ACHOQW_002018"
     CDS             16199..17593
                     /gene="wcaJ"
                     /locus_tag="ACHOQW_002018"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP9573101.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17595..19073
                     /gene="wzxC"
                     /locus_tag="ACHOQW_002019"
     CDS             17595..19073
                     /gene="wzxC"
                     /locus_tag="ACHOQW_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP9573102.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19253..20533
                     /gene="wcaK"
                     /locus_tag="ACHOQW_002020"
     CDS             19253..20533
                     /gene="wcaK"
                     /locus_tag="ACHOQW_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP9573103.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20530..21750
                     /gene="wcaL"
                     /locus_tag="ACHOQW_002021"
     CDS             20530..21750
                     /gene="wcaL"
                     /locus_tag="ACHOQW_002021"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP9573104.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21761..23155
                     /gene="wcaM"
                     /locus_tag="ACHOQW_002022"
     CDS             21761..23155
                     /gene="wcaM"
                     /locus_tag="ACHOQW_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP9573105.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23313..24308
                     /locus_tag="ACHOQW_002023"
     CDS             23313..24308
                     /locus_tag="ACHOQW_002023"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP9573106.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24551..25444
                     /gene="galF"
                     /locus_tag="ACHOQW_002024"
     CDS             24551..25444
                     /gene="galF"
                     /locus_tag="ACHOQW_002024"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP9573107.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6291 a         6333 c         6895 g         5981 t
ORIGIN      
        1 ttaatataat tcttaaaaat agccaattgc cgaattgtta ccttgcctgc tattccgtta
       61 gctgtaacac ttcctcctgc attattggag agccaatatt caattaccat aatgtcacct
      121 tatattcgga agttaatttc cgcaataacc ctttctcatc aacgattgta tgcagagagg
      181 gtttgaaaaa ttaagctaaa tcagtgtatt ggtagctaaa aagccagggg cggtagcgtg
      241 tctggatgcc tgaaagacca gtctgaatta ttctgtcaat agcctgcgga tgaaatcaat
      301 ttttttagga ctgatgccag ttaaattttt attcaattaa ctgcaaggta aatgtgcatg
      361 gcgacattgc tattaatagt gcacaggata attactctgc caaagtgata aataaacaat
      421 gatgaaatcc aaaatgaaat tgatgccatt attggtgtca gtaaccttga taagcggttg
      481 cacagtactt ccgggcagca atatgtcgac gatgggcaaa gacgtcatca aacagcagga
      541 cgctgatttc gatctcgaca aaatggtgaa tgtttatccg ctgaccccgc gcctgattga
      601 ccaattacgc ccacgcccga atgtagcgcg ccccaatatg acgctggaaa gtgagatcgc
      661 gaattaccag tatcgcgtcg ggccggggga cgttcttaat gtcaccgtct gggatcaccc
      721 ggaactcacc acgccagccg gtcagtaccg cagctccagc gacaccggca actgggtaca
      781 gcctgacggc actatgtttt acccgtatat cggcaaggtt cacgtagtcg ggaaaacgct
      841 cgctgaaatc cgcagtgata ttaccgggcg cttagcgacg tacatcgctg acccgcaggt
      901 ggacgttaat atcgccgcct tccgctcaca aaaggcctat atctcaggtc aggtgaataa
      961 atctggtcaa caggcgatca ccaacgtacc actgaccatt ctcgacgcca tcaacgccgc
     1021 aggtggcctg accgacaccg ctgactggcg caacgtggtg ctaacacaca atggtcgtga
     1081 agagcgaatt tctttgcagg cgctgatgca aaacggcgac ctcaatcaga accgactgct
     1141 ttaccccggc gatattctct acgtgccgcg taatgatgat ctgaaagtgt ttgtgatggg
     1201 tgaagtgaag aaacagagca ccctgaaaat ggactttagc ggcatgaccc tgaccgaagc
     1261 cctgggcaat gctgaaggca tcgacatgac cacctccaac gccagcggca tctttgtcat
     1321 tcgtccgctg aaaggcgagg gcgggcgtaa cggcaaaatt gccaatatct accagctgga
     1381 tatgtccgat gccacgtcgc tggtgatggc gacagaattc cgcctgcaac cttatgacgt
     1441 ggtgtatgtc accaccgccc cggtttcccg ctggaaccgt ctgatcaatc agttgctgcc
     1501 aactattagc ggtgtccgtt acatgacgga tacagccagc gacattcata actggtaatc
     1561 gtcatgttta acaacatctt agttgtctgt gtcggcaata tttgccgttc cccgacggcg
     1621 gaacgcttac tgcaacgtta tcacccggag ctgaaagtgg agtccgctgg actcggcgcg
     1681 ctggtcggta agggcgctga tcctaccgct atcagcgtcg ccgcagaaca tcaactgtct
     1741 ctggaaggcc actgtgcacg acaaatcagc cgccgcctgt gtcgcaacta tgacctgatt
     1801 ttgaccatgg aaaagcgcca tatcgaacgc ttatgcgaga tggcaccgga gatgcgcggc
     1861 aaagtgatgc tgtttggtca ctgggataac gaatgtgaaa tccccgatcc gtatcgcaaa
     1921 agccgggaaa cgtttgcagc ggtgtacaca ttacttgaac ggtctgcccg ccagtgggcg
     1981 caggcattga acgcagagca ggtataagaa tgacagaaaa agtaaaacaa catgccgctc
     2041 cggtaacggg cagtgatgaa atcgatattg gtcgcctggt cggcaccgtc attgaagcgc
     2101 gctggtgggt gattggcatc accgctgtat tcgccctttg tgccgtggtt tacaccttct
     2161 tcgccacgcc gatttatagt gccgacgcac tggtacaaat cgagcaaagc agcggcaatt
     2221 cgttagtgca ggatatcgga tcggcgttag ccaacaaacc gcctgcatcg gacgccgaga
     2281 tccagttgat tcgttcgcgc ctggtgcttg gtaaaacggt ggatgatctc gacctcgata
     2341 ttgcggtgag caaaaacacg ttcccgattt tcggtgcggg ctgggatcgc ctgatgggac
     2401 gccagaacga gacggtgaaa gtgactacct ttaaccgtcc gaaagagatg gaggatcagg
     2461 tgtttacgct taatgtgctg gacaacaaaa actacaccct gagcagcgat ggcggcttta
     2521 gcgcccgtgg gcaagcgggc cagatactga aaaaagaagg cgtcacgctg atggttgaag
     2581 ccattcacgc ccgcccgggc agtgagttta ccgtcaccaa atactccacg ctggggatga
     2641 tcaatcaact gcaaaacagc ctgacggtaa cggagaacgg caaagacgca ggcgtactga
     2701 gcctgactta taccggtgaa gatcgcgaac agatccgcga cattcttaac agcatcgccc
     2761 gtaactatca ggaacaaaat attgagcgca aatcggcgga agcgtcgaaa agcctcgctt
     2821 tcctcgcgca acagttaccg gaagtacgta gccgccttga tgttgccgaa aacaaactga
     2881 atgccttccg tcaggataaa gattctgttg atctgccgct ggaagcgaaa gcggtgctcg
     2941 attcgatggt gaacatcgac gcccagttga acgaactgac ctttaaagag gcggaaatct
     3001 ccaagctgta caccaaagtt caccccgcgt accgcacgct gctggagaaa cgtcaggcgc
     3061 tggaagacga aaaagccaaa cttaatggtc gcgtaacggc gatgccgaaa acccagcagg
     3121 aaattgtccg tctgacccgc gatgtcgagt ctggtcagca ggtctatatg caactgctga
     3181 ataaagagca ggagctgaaa atcaccgagg ccagcaccgt cggcgatgtg cgcattgttg
     3241 acccggcaat cactcagcct ggtgtgctaa aaccgaagaa agggctgatt atccttgggg
     3301 cgattatcct tggcctgatg ctctctatcg tgggggtgct gctgcgctcg ttgtttaatc
     3361 gcggcatcga aagcccgcag gtgctggaag aacacggtat cagcgtctat gccagcatcc
     3421 cgctgtcgga gtggcagaaa gcgcgcgata gcgtcaaaac catcaaaggg attaaacgct
     3481 ataaacagag ccagctactg gcggtgggga atccaaccga tctggcgatt gaagccatcc
     3541 gcagccttcg taccagtttg cacttcgcga tgatgcaggc gcagaacaat gtgttgatga
     3601 tgaccggggt tagcccgtca atcggtaaaa cctttgtctg cgccaacctg gcggcggtga
     3661 tcagccagac caataaacgc gtgttgttga tcgactgcga tatgcgcaaa ggctacaccc
     3721 atgagctgtt gggcactaat aacgttaatg gcctgtcgga aattctgatt ggtcagggcg
     3781 atattactac agctgctaaa ccgacctcta ttgccaaatt tgacctgatc ccgcgcggtc
     3841 aggtaccgcc aaatccttct gaactgttga tgagcgaacg ctttgccgaa ctggtgaact
     3901 gggcgagtaa aaactacgac ctggtgttga ttgatacgcc gccgattctg gcagtgaccg
     3961 atgcggcaat tgttggtcgt catgtcggaa ccacgttaat ggtggcgcgt tatgcggtca
     4021 acacattgaa agaagtggaa accagtctga gccgctttga gcaaaacggt attccggtga
     4081 aaggggtgat tctgaactcc atcttccgcc gcgccagcgc gtatcaggat tatggctatt
     4141 acgaatacga atataagtcg gatgcgaaat aacgaggcct gcattcgcac cgccccgtag
     4201 gccggataag gcgctcacgc cgcatccggc aagcaaacca gctcataagc cgggagtaca
     4261 acctatgaaa gacaatccgc tgatctcaat ctatatgccg acctggaacc gccaacaact
     4321 ggcgattcgg gcgataaaat cggtcttgcg ccaggactac agcaactggg agatgatcat
     4381 cgtggatgat tgctccacct cctgggaaca actgcaacag tacgtcaccg ccctcaacga
     4441 tccgcgtatt acctacattc acaacgacat aaacagcggg gcgtgcgcgg tacgtaacca
     4501 ggcgattatg ctggcgcaag gggaatacat taccgggatc gatgacgatg atgaatggac
     4561 gcccaaccgc ctgagcgtct tcctcgccca taaacagcaa ctggtcactc acgccttttt
     4621 gtacgctaac gattacgttt gccagggcga agtttattcc caaccggcaa gcttaccact
     4681 gtatcccaaa tcgccgtact cacgccgcct gttctataaa cgcaatatca ttggcaatca
     4741 ggtctttacc tgggcatggc gtttcaaaga gtgtttgttc gataccgaac tgaaagcggc
     4801 acaggattac gacatcttcc tgcggatggt ggtggagtat ggcgaaccgt ggaaagtgga
     4861 agaggcgacg cagatcctgc atatcaatca cggtgaaatg cagatcacct cgtcaccgaa
     4921 aaaattctcc ggatacttcc acttttaccg caagcacaaa gacaaattcg accgtgccag
     4981 taaaaaatat cagctgttta ccctctacca gatccgcaat aaacgtatga cctggcgcac
     5041 tttgctgacg ctgctgtcgg tgcgtaacgg caaacgtctg gctgatggta ttcgggggcg
     5101 ctaaatatgc tggaagatct acgcgccaac agctggagtt tacgcccgtg ctgcatggtt
     5161 cttgcctatc gcgtcgctca tttttgttcg gtgtggcgca aaaagaacgt tctcaacaat
     5221 ctttgggcgg ccccgctgct ggtgctgtat cgcattatca ccgaatgctt tttcggttat
     5281 gaaatccagg ctgccgcgac cattggccgc cgctttacta tccatcacgg ttacgccgta
     5341 gtgatcaata aaaacgtggt agcgggggat gatttcacca ttcgccacgg cgtcactatc
     5401 ggcaatcgtg gtgccgataa catggcatgt ccacacattg gcaacggcgt cgaactcggt
     5461 gccaacgtca ttattcttgg tgatatcacg cttggtaaca acgtcaccgt gggcgcgggc
     5521 agcgtggtgc tcgactctgt cccggacaac gcgctggtgg tgggagaaaa agcgcgagtg
     5581 aaggtaatta aatgaatatt ctgcaattta atgtgcgact ggcggaaggc ggggcggcag
     5641 gtgtggcgtt agatctccac cagcgtgcgc tgcaacaggg gctggcgtca cattttgtct
     5701 acggctacgg caaaggcggc aaagagagcg tcagccatca gaactatccg caggtcatca
     5761 aacatacgcc gcggatgacc gcaatggcga atattgccct gtttcgtctg tttaatcgcg
     5821 atctgtttgg caatttcaat gagttatatc gcaccattac tcgtacaccg ggtccggtgg
     5881 tcctgcattt tcatgtgctg cacagctact ggctaaatct taagagcgtg gtgcgctttt
     5941 gcgaaaaggt gaaaaaccac aaaccggatg tcactctggt ctggacgctg cacgaccact
     6001 ggagcgttac cgggcgctgc gcctttaccg acggttgtga aggctggaaa acgggctgcc
     6061 agaaatgccc gaccttaaat aactatccgc cggtgaagat tgatcgggcg caccagctgg
     6121 tggcgggcaa acgccagtta ttccgtgaga tgctggcgct gggctgtcag tttatttccc
     6181 ccagccagca tgtggctgac gctttcaata gcctgtacgg tccagggcgt tgccggatta
     6241 tcaataacgg tattgatatg gcaaccgagg cgattctggc ggatttgcct ccggtgcgcg
     6301 aaactcaggg caagccgaaa atcgcggtgg tagcgcatga cctgcgttac gacggcaaaa
     6361 ctaaccagca actggtacgc gagatgatgg cgctgggcga caaaatcgaa ctgcatacct
     6421 ttggtaagtt ctcgccgttc accgctggca acgtggttaa tcacggcttt gaaactgaca
     6481 agcgcaagtt gatgagcgcg ctcaatcaga tggatgcgct ggtgttcagt tctcgcgtcg
     6541 ataactaccc gctgattttg tgtgaggcgt tatcaattgg cgtgccggtg attgccaccc
     6601 atagcgacgc ggcgcgtgaa gtgttgcaaa aatccggcgg taaaaccgtc agcgaagaag
     6661 aggtgctgca actggtccag ttaagcaaac cggaaatcgc gcaggcgtta tttggtacca
     6721 cgctggctga gttcagccaa cgcagccgcg ccgcctacag tggacaacag atgctggagg
     6781 agtatgtcaa cttctatcag aatctgtagc tacctgctgc tgccgctgat ctacctgctg
     6841 gttaacgtca aaatcaccca gcttggcgaa agtttcccca tcaccatcgt cactttttta
     6901 cctgtcttgt tactgctgtt tttagaacgc atcagcgtta aaaaattgat gattgcctta
     6961 gggatttgcg cgggactcac ggcgtttaac tacctgtttg gtcagtcgct ggatgccagt
     7021 aaatacgtca cttcaactat gctgtttgtc tatatcgtga tcattattgg tatggtgtgg
     7081 agtattcgct ttaaaacgat ttcgccacac aaccatcgca agatattacg tttcttttat
     7141 ctggtggtcg ggctggtggt ggcgctggcg gcggtggaga tggcgcaaat tatcctcacc
     7201 ggcggcagca gtattatgga gtcgatttcg aaatatctga tttacagcaa cagctatgtg
     7261 ctgaatttca tcaaattcgg cggcaagcgc acgacagcac tttatttcga accggcattt
     7321 ttcgctctgg cattaatctc aatttggctc agcatcaaac agtttggtat caaaacgcct
     7381 aaaacagatg ctatgattct cgcagggata atattatccg gatcgttttc aggggttatg
     7441 acctttatcc tgttttattt gctggagtgg gcatttcaat atctgaataa agaggcgatt
     7501 aagaaaaagt taccgttagc attgatttct ctggctgtat tcctggttgg tgtggtaatt
     7561 gcatttcctt atatttccac ccgtctgggc gatttaggta cggaaggatc gtcatcgtat
     7621 tatcgtattg tcggtccgct ggtgatggtc ggttattctt tgacccatat tgacggtgta
     7681 gtcagatttg gctcacttta tgaatatgtc gcatcattcg gaatatttaa cggtgcggat
     7741 gtcggaaaaa ccatagacaa tggtttgtat ctgctgatta tttatttttc ttggttcgcg
     7801 gtgtttttat cgctgtggta catggggaaa gtgataaaaa tgatgatcaa cgcttttggt
     7861 gataaccgca attttcgcgt gcaattatat ctttttactc cggtatcgct gttttttacc
     7921 ggttcgatat ttagcccgga atatgcattt ttaatcgtct gtccgtttat tttgcgaaaa
     7981 gcgttaaata ttacgaggta agaataagaa catgttgctt agcataatca ctgtcgcgtt
     8041 tcgtaacctc gaagggatag tcaaaacaca tgcctcgctg gcgcatctgg cgcaggcgga
     8101 agatatcagc ttcgaatgga ttgttgtcga tggcggttcc aatgacggca ctcgtgagta
     8161 cctggaaaat ctcaatggta tctataacct acgctttgtc agcgagccag ataacggtat
     8221 ctacgacgcc atgaacaagg gcattgcgat ggcacaaggc aagttcgcgt tgtttctcaa
     8281 ctcgggcgat atttttcatc aggatgccgc atattttgtc cgtaagttaa aaatgcaaaa
     8341 agataacgtg atgatcaccg gcgatgcgct gctggatttt ggcgacgggc ataaaattaa
     8401 acgtagcgcc aaaccgggct ggtatattta tcacagcctg cccgccagtc atcaggcgat
     8461 atttttcccg gtatccggtt tgaaaaaatg gcgttatgac ctggaatata aagtttcttc
     8521 tgattacgcg ctggcagcca aaatgtataa agcaggttat gcatttaaaa aactcaatgg
     8581 cctggtgtct gaattttcta tgggtggggt atctaccacc aataatatgg aattgtgtgc
     8641 tgacgcgaaa aaagtccaac ggcaaatatt acatgtgcct ggcttttggg ctgaattatc
     8701 ctggcattta cgccagcgta ctacctcaaa gacgaaagcc ttatataaca aaagctgaat
     8761 ataagggaaa acgatgcagg atttaagcgg tttctcggtg ccgaaagggt tccggggcgg
     8821 caacgctatt aaagtgcaat tgtggtgggc agtacaggca acaatatttg cctggtcgcc
     8881 acaagtattg tatcgctggc gggcattttt attacgttta ttcggcgcaa aaataggaaa
     8941 aaacgtagtt attcgtccgt cagtaaaaat tacctatccg tggaaattaa ccttaggtga
     9001 ttacgtgtgg gtcggcgatg acgtcaattt atataccctc ggtgaaataa ccattggcgc
     9061 acattcggtg atatcgcaaa aaagttattt atgcaccggg agccacgacc atgcaagtca
     9121 acatttcacc attaacgcca cgcctattgt gattggcgag aaatgctggc tggcaaccga
     9181 tgtttttgtt gccccaggcg tcacgattgg cgacggcacc gtcgtgggtg cgcgaagcag
     9241 tgtttttaaa tcgcttccgg caaatgtggt ttgccggggg aatcccgcag tggtgatacg
     9301 caaacgcgtt gaaactgaat aaattcaaaa aaaatacaga ggaataagac atgtcaaaag
     9361 tcgctctcat caccggtgta accggacaag acggttctta cctggcagag tttctgctgg
     9421 aaaaaggtta cgaggtgcat ggtattaagc gtcgcgcatc gtcattcaac accgagcgcg
     9481 tggatcacat ttatcaggat ccgcacacct gcaacccgaa attccatctg cattatggcg
     9541 acctgagtga tacctccaac ctgacgcgta ttttgcgtga agtgcagccg gatgaagtgt
     9601 acaacctggg cgcaatgagc cacgttgcgg tttcttttga gtcaccggaa tataccgctg
     9661 acgtcgacgc gatgggtacg ctgcgcctgc tggaggcgat ccgcttcctc ggtctggaaa
     9721 agaaaacccg tttctatcag gcttccacct ctgaactgta tggtctggtg caggaaattc
     9781 cgcagaaaga gaccacgccg ttctatccgc gctctccgta tgcggtcgcc aaactgtatg
     9841 cctactggat caccgtcaac taccgtgaat cctacggcat gtatgcctgt aacggtattc
     9901 tcttcaacca tgaatccccg cgccgcggcg aaaccttcgt tacccgcaaa atcacccgcg
     9961 caatcgccaa catcgcccag gggctggagt cgtgcctgta cctcggcaat atggattccc
    10021 tgcgtgactg gggccatgcc aaagactacg taaaaatgca gtggatgatg ctgcaacagg
    10081 aacagccaga agatttcgtt atcgcgaccg gcgttcagta ctccgtacgt cagttcgtag
    10141 aaatggcggc agcacagctg ggcatcaaac tgcgctttga aggcacgggc gttgaagaga
    10201 agggcattgt ggtttccgtc accgggcatg acgcgccggg tgttaaaccg ggtgatgtga
    10261 ttatcgccgt tgatccgcgt tacttccgtc cggctgaagt tgaaacgctg ctcggcgacc
    10321 cgaccaaagc gcacgaaaaa ctgggctgga aaccggaaat caccctcaga gagatggtgt
    10381 ctgaaatggt ggctaatgac ctcgaagcgg cgaaaaaaca ctctctgctg aaatctcacg
    10441 gctacgacgt ggcgatcgcg ctggagtcat aagtatgagt agacaacgca tttttattgc
    10501 tggccatcgc gggatggtcg gttccgccat caggcggcag ctcgaacagc gcggtgatgt
    10561 ggaacaggta ttacgcaccc gcgacgagct gaacctgctg gacagccgcg ccgtgcatga
    10621 tttctttgcc agcgaacgta ttgaccaggt ctatctggcg gccgcgaaag tgggcggcat
    10681 tgttgccaac aacacgtatc cggcggattt catctatcag aacatgatga ttgagagcaa
    10741 catcattcac gccgcgcatc agaacgacgt gaacaaactg ctgtttctcg gatcgtcctg
    10801 catctacccg aaactggcaa aacagccgat ggcagaaagc gagttattgc agggcacgct
    10861 ggagccgacc aacgagcctt acgccattgc caaaatcgcc gggatcaaac tgtgcgaatc
    10921 atacaaccgc cagtacggac gcgattaccg ctcagtcatg ccgaccaacc tgtatggccc
    10981 gcatgacaac ttccacccga gtaattcgca tgtgatccct gcattgctgc gtcgcttcca
    11041 cgaggcgacg gcacagaatg caccggacgt ggtggtgtgg ggcagcggta cgccgatgcg
    11101 agaatttctg cacgtcgatg atatggcggc ggcgagcatt catgtcatgg agctggcgca
    11161 tgaagtctgg ctggagaaca cccagccgat gctgtcgcac attaacgtcg gcacgggcgt
    11221 tgactgcact atccgcgagc tggcgcaaac catcgccaaa gtggtgggtt acaaaggtcg
    11281 ggtggttttt gatgccagca aaccggatgg tacgccgcgc aaactgctgg atgtgacgcg
    11341 cctgcatcag cttggctggt atcacgaaat ctcactggaa gcggggcttg ccagcactta
    11401 ccagtggttc cttgagaatc aagaccgctt tcgggggtaa tgatgttttt acgtcaggaa
    11461 gactttgcca cggtagtgcg ctccacaccg cttgtctctc tcgactttat tgtcgagaac
    11521 agtcgcggcg agtttctgct tggcaaaaga accaaccgcc cggcgcaggg ttactggttt
    11581 gtgccgggag ggcgcgtgca gaaagacgaa acgctggaag ccgcatttga gcggctgacg
    11641 atggcggaac tggggctgcg tctgccgata acagcaggcc agttttacgg tgtctggcag
    11701 cacttttatg acgataactt ctccggcacg gatttcacca ctcactatgt ggtgctcggt
    11761 tttcgcttca gagtagcgga agaagaactg ttactgccgg atgagcagca tgacgattac
    11821 cgctggctga cgccggacgc gctgctcgcc agtaacgatg ttcacgctaa cagccgcgcc
    11881 tattttctcg ctgagaagcg tgccggagta cccggattat gaaaatactg gtctacggca
    11941 ttaactactc gccggagtta accggcatcg gcaaatacac cggcgagatg gtggaatggc
    12001 tggcggcgca aggtcatgag gtgcgggtta ttaccgcacc gccttactac ccgcagtggc
    12061 aagtgggcga gaactattcc gcctggcggt acaaacgaga agagggggcc gctacggtgt
    12121 ggcgctgccc gctgtacgtg ccaaaacagc cgagcaccct gaaacgcctg ttgcatctcg
    12181 gcagttttgc cgtcagcagt tttttcccgt tgatggcgca acgtcgctgg aagccggatc
    12241 gcattatcgg cgtagtgcca acgctgtttt gcacgccggg aatgcgcctg ctggcgaaac
    12301 tctctggcgc gcgtaccgtg ctgcatattc aggattacga agtagatgcc atgctggggc
    12361 tgggccttgc cggaaaaggc aaaggcggca aagtggcaca gctggcaacg gcgttcgaac
    12421 gtagcggact gcataacgtc gataacgttt ccacgatttc gcgttcgatg atgaataaag
    12481 ccatcgaaaa aggcgtggcg gcggaaaacg tcatcttctt ccccaactgg tcggaaatcg
    12541 cccgttttca gcatgttgca gacgccgatg ttgatgccct tcgtaaccag cttggcctgc
    12601 cggataacaa aaaaatcatt ctttactccg gcaatattgg tgaaaagcag gggctggaaa
    12661 acgttattga agcagccgat cgcctgcgcg atgaaccgct gatttttgcc attgtcgggc
    12721 agggcggcgg caaagcgcgg ctggaaaaaa tggcgcagca gcgtggactg cgcaacatgc
    12781 aatttttccc gctgcaatcg tatgacgctt tacccgcact gctgaagatg ggcgattgcc
    12841 atctggtggt gcaaaaacgc ggcgcggcag atgccgtatt gccgtcgaaa ctgaccaata
    12901 ttctggcagt aggcggtaac gcggtgatta ctgctgaagc ccacacagaa ctgggacagc
    12961 tttgcgaaac ctttccgggc attgcggttt gcgtagaacc ggaatcggtt gaggcgctgg
    13021 tggcggggat tcgtcaggcg ctcctgctgc ccaaacacaa cacggtggca cgtgaatatg
    13081 ccgaacgcac gctcgataaa gagaacgtgt tacgtcaatt tataaatgat attcggggat
    13141 aattatggcg cagtcgaaac tctatccagt tgtgatggca ggtggctccg gtagccgctt
    13201 atggccgctt tcccgcgtac tttatcccaa gcagttttta tgcctgaaag gcgatctcac
    13261 catgctgcaa accaccatct gccgcctgaa cggcgtggag tgcgaaagcc ctgtggtgat
    13321 ttgcaatgag cagcaccgct ttattgttgc ggaacaactg cgtcaactga acaaactcac
    13381 tgagaacatt attctcgaac cggcagggcg taacacagca ccggccattg cgctggcggc
    13441 gctggcggca aaacgtcata gcccggagaa cgacccatta atgctggtgc tggcggcgga
    13501 tcatgtgatt gccgatgaag acgcattccg cgccgccgtg cgtaacgcta tgccgtatgc
    13561 cgaagcgggc aagctggtga ccttcggcat tgtgccggat ctacctgaaa ccggatatgg
    13621 ctatattcgt cgcggtgaag tgtcggcggg tgagcaggat acggtggcct ttgaagtggc
    13681 gcagtttgtc gaaaaaccaa atctggaaac cgcgcaggcc tatgtggcaa gcggcgaata
    13741 ttactggaac agcggtatgt tcctgttccg tgccggacgc tatctcgaag aactgaaaaa
    13801 atatcgcccg gatattctcg acgcctgtga aaaagcgatg agcgccgtcg atccggatct
    13861 cgattttatt cgcgtggatg aagacgcgtt tctggcctgc ccggaagagt cggtggatta
    13921 cgaggtcatg gaacgtacgg cagatgccgt tgtggtgccg atggatgcgg gctggagcga
    13981 tgtcggctcc tggtcctcat tatgggagat cagcgcccac accgccgagg gcaacgtttg
    14041 ccacggcgat gtgattaatc acaaaactga aaacagctat gtgtatgccg aatctggcct
    14101 ggtcaccacc gtcggggtga aagatttggt ggtagtgcag accaaagatg cggtgctgat
    14161 tgccgaccgt aacgcggtgc aggatgtgaa aaaagtggtc gagcagatca aagccgatgg
    14221 tcgccatgag catcgggtgc atcgcgaagt ctatcgcccg tggggcaaat atgactctat
    14281 cgacgcgggc gaccgctacc aggtgaaacg catcaccgtg aaaccgggcg aaggcttgtc
    14341 ggtacagatg caccatcacc gcgcggaaca ctgggtggtt gtcgcgggaa cggcaaaagt
    14401 caccattgac ggtgatatca aactgcttgg tgaaaacgag tcgatttata ttccgctggg
    14461 ggcgacgcac tgcctggaaa acccggggaa aattccgctc gatttaattg aagtgcgctc
    14521 cggctcttat ctcgaagagg atgatgtggt gcgcttcgcg gatcgctacg gacgggtgta
    14581 aacgtcgcat caggcgctga ttgtcggatg cggcgtgaac gccttatccg acctacggtt
    14641 cggttttgta ggcctgataa gacgcggcag cgtcgcatca ggcaatgaat gcgaaaccgc
    14701 ggtgtaaata acgacaaaaa caaaattggc cgtttcggtc agggccaact attgcctgaa
    14761 aaagggtaac gatatgaaaa aattaacctg ctttaaagcc tatgatattc gcgggaaatt
    14821 aggcgaagaa ctgaatgaag atattgcctg gcgcattggt cgtgcctatg gcgaatttct
    14881 caaaccgaaa accattgtgt taggcggtga tgcccgtctc accagcgaaa ccttaaaact
    14941 ggcgctggca aaaggtttac aggatgcggg cgttgacgtg ctggatattg gtatgtccgg
    15001 caccgaagag atctatttcg ccacgttcca tctcggcgtg gatggcggca ttgaagttac
    15061 tgccagccat aacccgatgg attacaacgg catgaaactg gtgcgcgaag gggctcgccc
    15121 gatcagcggc gataccggac tgcgcgatgt ccagcgtctg gcagaagcca acgactttcc
    15181 tcccgttgat gaaaccaaac gcggtcgcta tcagcaaatc aatctgcgtg acgcttacgt
    15241 tgatcacctg ttcggttata tcaacgtcaa aaaccttacg ccgctcaagc tggtgatcaa
    15301 ctccgggaac ggcgcggcgg gtccggtggt ggacgccatc gaagcccgct ttaaagccct
    15361 cggcgcaccg gtggaattga tcaaagtgca caacacgccc gacggcaatt tccccaacgg
    15421 tattcctaac ccgctgctgc cggaatgtcg cgacgacacc cgcaatgcgg tcattaaaca
    15481 cggcgcggat atgggcattg cttttgatgg cgattttgac cgctgtttcc tgtttgacga
    15541 aaaagggcag tttatcgagg gctactacat tgtcggtctg ctggcagaag cgttcctcga
    15601 aaaaaatccc ggcgcgaaga tcatccacga tccacgtctc tcttggaata ccgttgatgt
    15661 ggtgactgcc gcaggcggca ccccggtaat gtcgaaaacc ggacacgcct ttattaaaga
    15721 acggatgcgc aaggaagacg ccatctacgg tggcgaaatg agcgcccacc attacttccg
    15781 tgattttgct tactgcgaca gcggcatgat cccgtggctg ctggtcgccg aactggtgtg
    15841 cctgaaagga aagacgctgg gcgaactggt gcgcgaccgg atggcagcgt ttccgtcaag
    15901 cggtgagatc aacagcaaac tggcgcaacc cgttgaggcg attaaccgcg tggaacagca
    15961 ttttagccgt gaggcgctgg cggtggatcg caccgatggc atcagcatga cctttgccga
    16021 ctggcgcttt aatctgcgct cctccaacac cgaaccggtg gtgcggttga atgtggaatc
    16081 acgcggtgat gtgccgctga tggaagcgcg aacgcgaact ctgctgacgt tgctgaacga
    16141 gtaatgtcgg atcttccctt accccattgc gggtaagggg ctaataacag gaacaacgat
    16201 gacaaatcta aaaaagcgcg agcgagcgaa aaccaatgca tcgttaatct ctatggtgca
    16261 acgcttttca gatatcacca tcatgtttgc cggactatgg ctggtttgcg aagtgagcgg
    16321 actgtcattc ctctacatgc acctgttggt ggcgctgatt acgctggtgg tgttccagat
    16381 gctgggcggc atcaccgatt tttatcgctc atggcgcggt gttcgggcag cgacagaatt
    16441 tgccctgttg ctacaaaact ggaccttaag cgtgattttc agcgccggac tggtggcgtt
    16501 caacaatgat ttcgacacgc aactgaaaat ctggctggcg tggtatgggc tgaccagtat
    16561 cggactggtg gtttgccgtt catgtattcg cattggggcg ggctggctgc gtaatcatgg
    16621 ctataacaag cgcatggtcg cggtggcggg ggatttagcc gccgggcaaa tgctgatgga
    16681 gagcttccgt aaccagccgt ggttagggtt tgaagtggtg ggcgtttacc acgacccgaa
    16741 actgggcggc gtttctaacg actgggcggg taacctgcaa cagctggtcg aggatgctaa
    16801 agcaggcaag attcataacg tctatatcgc gatgcaaatg tgcgacggcg cgcgagtgaa
    16861 aaaactggtc catcaactgg cggacaccac ctgttcggtg ctgctgatcc ccgatgtctt
    16921 taccttcaac attctccatt cacgtcttga agagatgaac ggcgttccgg tggtgccgct
    16981 ttacgacacg ccgctttccg gggttaaccg cctgctcaaa cgtgcggaag acattgtgct
    17041 ggcgacgctt atcctgctgc tgatctcccc ggtgctgtgc tgcattgcgc tggcggtgaa
    17101 actcagttca cctgggccgg ttattttccg ccagactcgc tacggcatgg atggcaagcc
    17161 gatcaaagtg tggaagttcc gttccatgaa agtgatggag aacgacaaag tggtgaccca
    17221 ggcgacgcag aacgatccgc gcgtcaccaa agtggggaac tttctgcgcc gcacctcgct
    17281 ggatgaattg ccgcagttta tcaatgtgct gaccgggggg atgtcgattg tcggtccacg
    17341 tccgcacgcg gtggcgcata acgaacagta tcgacagctc attgaaggct acatgctgcg
    17401 tcataaggtg aaaccgggca ttaccggctg ggcgcagatt aacggctggc gcggcgaaac
    17461 cgacacgctg gagaaaatgg aaaaacgcgt cgagttcgac cttgagtaca tccgcgaatg
    17521 gagcgtctgg ttcgatatca aaatcgtttt cctgacggta ttcaaaggat tcgttaacaa
    17581 agcggcatat tgatatgagc ttacgtgaaa aaaccatcag cggcgcgaag tggtcggcga
    17641 ttgccacggt gatcatcatc ggcctcgggc tggtgcagat gaccgtgctg gcgcggatta
    17701 tcgacaacca ccagttcggt ttgcttaccg tgtcgctggt gatcatcgcg ctggcagata
    17761 cgctttctga cttcggtatt gctaactcga ttattcagcg aaaagaaatc agccaccttg
    17821 aactcaccac gctgtactgg ctgaacgttg ggctggggct tgtggtgtgt gtggcggtgt
    17881 ttttgttgag tgatctcatt ggtgacgtgc tgaacaaccc ggacctggca ccgttgatta
    17941 aaacattgtc gctggcgttt gtggttatcc cccacgggca acagttccgc gcgttgatgc
    18001 aaaaggagct ggagttcaac aaaatcggca tgatcgaaac cagcgcggta ctggcgggct
    18061 tcacttttac ggtggttagc gcccatttct ggccgctggc gatgaccgcg atcctcggtt
    18121 atctggtcaa tagtgcggtg agaacgctgc tgtttggcta ctttggccgc aaaatttatc
    18181 gccccggtct gcatttctcg ctggcgtctg ttgcgccgaa cttacgcttt ggtgcctggc
    18241 tgacggcgga cagcatcatc aactatctca ataccaacct ttcaacgctg gtgctggcgc
    18301 gtattctcgg cgcgggcgtg gcagggggat acaacctggc gtacaacgtg gccgttgtgc
    18361 cgccgatgaa gctgaacccg atcatcaccc gtgtgttgtt tccggcattc gccaaaattc
    18421 aggacgatac cgaaaagctg cgcgtcaact tctacaagct gctgtcggtg gtggggatta
    18481 tcaactttcc ggcgctgctc gggctgatgg tggtgtcaaa taactttgta ccgctggtat
    18541 ttggcgaaaa gtggaacagc attattccgg tgctgcaatt gctgtgtgtg gtgggtctgc
    18601 tgcgctccgt aggtaacccg attggttcgc tgctgatggc gaaagcgcgg gtcgatatca
    18661 gctttaaatt caacgtattc aaaacatttc tgtttattcc ggcgattgtt attggtgggc
    18721 agatggcggg cgcaatcggc gtcacgctcg gtttcctgct ggtgcagatt atcaacacca
    18781 ttctgagtta cttcgtgatg attaaaccgg ttcttggttc cagttatcgc cagtacatcc
    18841 tgagtttgtg gctgccgttt tatctctcgc tgccgacgct ggtggtcagt tatgtgctgg
    18901 gcattgtgct gaaagggcaa ctggcgctgg ggatgctgct ggcggtgcaa atagccgcgg
    18961 gcgtgctggc gtttgtggtg atgattgtgc tgtcgcgcca tccgctggtg gtggaagtga
    19021 agcgccagtt ttgtcgcagc gaaaaaatga aaatgctttt acgggcgggg tgagttgttg
    19081 ttttcggttc cgacaacggt tgccgggtgc agcgtaaaac accttatcca tcctacggtt
    19141 ctgtgctcgg gtcttgtagg cctgataaga cgcgccagcg tcgcatcagg caaaaaacgg
    19201 caacgaacac aacaaacaca gcctcacctg gcatcaaatt tatgaggaaa aaatgaaatt
    19261 acttattctg ggcaaccaca cttgcggcaa tcgtggcgac agcgccatct tgcgcggctt
    19321 acttgatgcc atcaacatcc tcaatccaca taccgaagtg gacgtgatga gccgctatcc
    19381 ggtcagttct tcctggctgc tcaaccgccc ggtaatgggt gatccgctgt tccttcaaat
    19441 gaaacaacac aacagcgcgg cgggcgttgt cgggcgcgtt aaaaaagtcc tccgtcgccg
    19501 ctatcagcac caggtattgc tctcacgcgt caccgacact ggcaagctgc gcaatatcgc
    19561 catcgcccag ggattcaccg acttcgtgcg cctactgtca ggttacgacg ccattattca
    19621 ggtcggcgga tcgttttttg tcgatctcta cggcgtgccg cagtttgaac atgcactttg
    19681 cacatttatg gcgaaaaagc cgctgtttat gattggtcac agcgtcggtc ccttccagga
    19741 tgagcaattt aaccaactgg cgaactacgt ttttggtcac tgcgacgcgc tgatcttgcg
    19801 cgaatcggtc agcctcgatc taatgaaacg cagcaatatc accactgcaa aagttgaaca
    19861 tggcgtcgat accgcgtggc tggtcgatca ccacacagaa gacttcaccg ccagctatgc
    19921 cgtccaacac tggctggacg ttgccgcaca acagaaaacg gtggcaatta ccctgcgcga
    19981 actggcaccg ttcgacaaac gcctcggcac cactcagcaa gtgtatgaaa aagcctttgc
    20041 cggggtggtc aatcgcattc tcgacgaagg ctatcaggtc attgcgctct ccacctgtac
    20101 gggtatcgac agctataaca aagacgaccg catggtggcg ctcaacctgc gccagcacat
    20161 cagcgatcct gcccgttatc acgtagtgat ggatgaactt aacgatctgg aaatgggcaa
    20221 aattctcggt gcctgtgaac tcaccgtcgg tacgcgcctg cactccgcca ttatctcaat
    20281 gaactttgct actccggcga ttgccatcaa ctatgaacat aaatccgccg gggttatgca
    20341 gcagctggga ctaccggaga tggcaattga tatccgtcat ttattagacg gcagcctgca
    20401 agcgatggtt gcggatacct taggccagct tccggcgctg aacgcacgac ttagcgaagc
    20461 cgtcagtcgt gagcgtcaga cgggaatgca gatggtgcag tccgtactgg aacgcatcgg
    20521 ggaggtgaaa tgaaggtcgg cttcttctta ttgaaatttc cgctgtcgtc agaaaccttc
    20581 gttcttaacc aaatcactgc gtttattgat atgggctttg aagtggagat tgtcgcgctg
    20641 caaaaaggcg acacacaaaa cacccacgtg gcatggacta aatacaacct tgccgccaga
    20701 acccgctggt tacaggacga acctacgggc aaagtggcga aactgcgcca ccgagccagt
    20761 cagaccttgc gcggcattca tcgtaaaaat acctggcagg cgctcaacct caaacgctat
    20821 ggtgccgagt cgtggaacct gattttgtct gccatttgcg gccaggtcgc aacaccgttt
    20881 cgcgccgatg tgttcatcgc tcattttggc cctgcggggg taaccgcagc aaaactacgc
    20941 gaactgggtg tcattcacgg caaaattgcc actattttcc acggcattga tatttccagt
    21001 cgggaagtgc tcaaccatta cactcccgaa tatcaacaac tgtttcgacg tggcgacctg
    21061 atgctaccga taagcgatct gtgggccgga aggctgcaaa aaatgggctg cccgagggaa
    21121 aaaatcgccg tatcgcgcat gggcgtagat atgacgcgct ttagcccgcg tcccgtgaaa
    21181 gcgcccgcaa caccgctgga gattatttcc gtcgcacgct taaccgagaa aaaaggtctg
    21241 catgtggcga ttgaagcctg ccggcagttg aaagagcagg gcgtggcatt tcgctatcgc
    21301 atcctcggca ttggcccgtg ggaacgacgc ctgcgcacgc tcatcgaaca atatcaactg
    21361 gaagatgtgg tggagatgcc gggctttaaa ccgagccatg aagtgaaagc gatgctcgac
    21421 gacgcggatg tcttcctgtt gccatcggtt acaggtgcgg atggcgatat ggaaggcatt
    21481 ccggtggcgc tgatggaggc gatggcggtc ggcattccgg tggtttctac tctgcatagc
    21541 ggaataccgg aactggtgga ggctgacaaa tccggctggc tggtgcctga gaacgatgct
    21601 cgcgcactgg cgcaacgact ggcggcgttt agccaactgg acaccgacga attggctccg
    21661 gtcgtcaaac gcgcgcgcga aaaagttgaa cacgatttta accagcaggt gatcaatcga
    21721 gaactcgcca gcttgctgca ggctttatag aggagaacgt atgccattta aaaaactctc
    21781 ccgacgcacc ttcctgacgg caagctcggc gcttgccttc cttcataccc ctttcgctcg
    21841 cgcacttccc gcccgacaaa gcgttaatat caacgactac aaccctcacg actggatcgc
    21901 ctcatttaaa caagccttca gcgaagggca aacggttgtc gtgcctgctg gattcgtttg
    21961 tgacaatatc aacaccggca tcttcattcc tcctggcaaa acgttacaca tccttggaag
    22021 cctgcgcggc aacggcagag ggcgatttgt cttacaggac ggcagccagg tgacagggga
    22081 ggagggcggc agtatgcata acatcaccct ggatgtgcgt ggttctgact gcaccatcaa
    22141 agggctggcg atgagcggct ttggtccggt aacgcagatt tatatcggcg gcaaaaacaa
    22201 acgggtcatg cgcaacctga ccatcgataa cctgaccatt agccacgcta attacgccat
    22261 cttacgccag ggatttcata accagattat cggtgccaac atcaccaatt gtaagttcag
    22321 cgacttacag ggcgatgcca ttgaatggaa cgtggcgatt aacgaccgcg atattttgat
    22381 ctccgaccat gtcatcgagc gcatcaactg tactaacggc aaaatcaact ggggcattgg
    22441 cataggcctt gcgggaagca cttacgataa taattacccg gaagatcagg cagtgaaaaa
    22501 ctttgtcgtg gcgaatatca cgggatcgga ttgtcggcag ttgatacatg ttgaaaatgg
    22561 taaacatttt gttattcgta atatcaaagc ccgcaatatc acgcaggatt tcagtaagaa
    22621 agcaggtatt gataacgcca cagtcgctat ttacggttgt gacaatttcg tgattgataa
    22681 tattgaaatg attaatagcg ccgggatgtt aatcggctat ggggtaatta aaggcaatta
    22741 tctctcgatt ccgcaaaatt tccgagtgaa taatattcaa ctggataaca cccatcttgc
    22801 ttataaattg cgcggcctcc aaatctctgc cgggaatgcc gtctcctttg tggcactaac
    22861 taacattgag atgaagcgtg cgtcgctgga gttacacaac aaaccgcaac atctttttct
    22921 gcgtaatatc aaagtgatgc aggaatcctc tgttggaccc gcattgatta tgaacttcga
    22981 catgcgcaaa gacgttcgag gcgtctttat ggcgaaagaa gaaacactgc tgtctcttgc
    23041 aaatgttcat gcggtgaatg agaaaggaca aagctccgtc gatatcgaca ggattaatca
    23101 ccatattgtt aatgtggaaa agattaactt tagattgccg gaacggagag agtagatttg
    23161 cgaccattca taaacttact ggatataatg ttctaaaaga ttataatcct cacactaact
    23221 gttaaagctg gggctgggca gcattgacta ctcagggaag gtactcatct ttatcttgtg
    23281 tttcttacgt aacaaccaga ctaaagtgat ttatgaacga taacgttttg ctcataggag
    23341 cttccggatt cgtaggaacc cgactacttg aaacggcaat tgctgacttt aatatcaaga
    23401 acctggacaa acagcagagc cacttttatc cagaaatcac acagattggt gatgttcgtg
    23461 atcaacaggc actcgaccag gcgttagccg gttttgacac tgttgtacta ctggcagcgg
    23521 aacaccgcga tgacgtcagc cctacttctc tctattatga tgtcaacgtt cagggtaccc
    23581 gcaatgtgct ggcggccatg gaaaaaaatg gcgttaaaaa tatcatcttt accagttccg
    23641 ttgctgttta tggtttgaac aaacacaacc ctgacgaaaa ccatccacac gaccctttca
    23701 accactacgg caaaagcaag tggcaggcgg aggaagtgct gcgtgaatgg tataacaaag
    23761 caccaacaga acgttcatta actatcatcc gtcctaccgt tatcttcggt gaacgcaacc
    23821 gcggtaacgt ctataacttg ctgaaacaga tcgctggcgg caagtttatg atggtgggcg
    23881 cagggactaa ctataagtcc atggcttatg ttggaaacat tgttgagttt atcaagtaca
    23941 aactgaagaa tgttgccgca ggttacgagg tttataacta cgttgataag ccagacctga
    24001 acatgaacca gttggttgct gaagttgaac aaagcctgaa caaaaagatc ccttctatgc
    24061 acttgcctta cccactagga atgctgggtg gatattgctt tgatatcctg agcaaaatta
    24121 cgggcaaaaa atacgctgtc agctctgtgc gcgtgaaaaa attctgcgca acaacacagt
    24181 ttgacgcaac gaaagtgcat tcttcaggtt ttgtggcacc gtatacgctg tcgcaaggtc
    24241 tggatcgaac tctgcagtat gaattcgtcc atgccaaaaa agacgacata acgtttgttt
    24301 ctgagtaatg cagacgagtc agagaaaaaa ggaccaggtt ggactacgat tattcggaaa
    24361 gaggtactga ttaaattatc agtatgtgga tttccgagta atccgagtaa cccagatgct
    24421 gatggtgtgt agtgttatcg taaggtttaa tcaaagttat gcggaactaa gctttggtat
    24481 atagccataa cgtttggcta acctatggca ataatttacg cactatcagc cacttagagg
    24541 taggaaaaga atgacgaatt taaaagcagt tataccggta gcaggtcttg ggatgcatat
    24601 gttgcctgcc actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat
    24661 tcagtacatt gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca
    24721 cgcgtccaag aacgcagtcg aaaaccactt cgacacctct tatgaattag aatctctcct
    24781 tgaacagcgc gtgaagcgtc aactactggc ggaagtacaa tctatctgtc cgccgggcgt
    24841 gaccattatg aacgtgcgtc agggtgaacc tttaggttta ggccactcca ttttatgtgc
    24901 gcgacctgcc attggtgaca atccatttgt cgtggtgctg ccagacgttg tgatcgacga
    24961 cgccagcgcc gacccgctac gctacaacct tgctgccatg attgcgcgct tcaatgaaac
    25021 gggacgcagc caggtgctgg caaaacgtat gccgggcgac ctctctgaat actccgtcat
    25081 ccagaccaaa gaaccgctgg atcgtgaagg caaagtcagc cgcattgttg aatttatcga
    25141 aaagccggat cagccgcaga cgctggactc agacatcatg gccgttggtc gatatgtgct
    25201 ttctgccgat atttggccgg aactggaacg tactcagcct ggtgcatggg gacgtattca
    25261 gctgactgat gccattgctg agctggcaaa aaaacaatcc gttgatgcaa tgctgatgac
    25321 tggcgacagt tacgactgcg gtaaaaaaat gggctatatg caggcgtttg tgaagtatgg
    25381 actacgcaac ttgaaagaag gggcgaagtt ccgcaaaggt atcgagaagc tgttaagcga
    25441 ataatgaaaa tctgaccgaa tgtaacggtt gataaggaaa taatatcggc agtgaaaaac
//
LOCUS       DAZKFZ010000055        25205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000055 DAZKFZ010000000
VERSION     DAZKFZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            293..1117
                     /gene="bax"
                     /locus_tag="ACHOQW_002025"
     CDS             293..1117
                     /gene="bax"
                     /locus_tag="ACHOQW_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP9573108.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(1225..2403)
                     /gene="xylR"
                     /locus_tag="ACHOQW_002026"
     CDS             complement(1225..2403)
                     /gene="xylR"
                     /locus_tag="ACHOQW_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP9573109.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(2481..3662)
                     /gene="xylH"
                     /locus_tag="ACHOQW_002027"
     CDS             complement(2481..3662)
                     /gene="xylH"
                     /locus_tag="ACHOQW_002027"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP9573110.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(3640..5181)
                     /gene="xylG"
                     /locus_tag="ACHOQW_002028"
     CDS             complement(3640..5181)
                     /gene="xylG"
                     /locus_tag="ACHOQW_002028"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP9573111.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(5259..6251)
                     /gene="xylF"
                     /locus_tag="ACHOQW_002029"
     CDS             complement(5259..6251)
                     /gene="xylF"
                     /locus_tag="ACHOQW_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9573112.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6617..7939
                     /gene="xylA"
                     /locus_tag="ACHOQW_002030"
     CDS             6617..7939
                     /gene="xylA"
                     /locus_tag="ACHOQW_002030"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP9573113.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8011..9465
                     /gene="xylB"
                     /locus_tag="ACHOQW_002031"
     CDS             8011..9465
                     /gene="xylB"
                     /locus_tag="ACHOQW_002031"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9573114.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            9634..9975
                     /gene="yiaB"
                     /locus_tag="ACHOQW_002032"
     CDS             9634..9975
                     /gene="yiaB"
                     /locus_tag="ACHOQW_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP9573115.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            10021..10458
                     /gene="yiaA"
                     /locus_tag="ACHOQW_002033"
     CDS             10021..10458
                     /gene="yiaA"
                     /locus_tag="ACHOQW_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP9573116.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQRNIRDAGINPPKETQVTQEEYSE"
     gene            complement(10500..11495)
                     /gene="wecH"
                     /locus_tag="ACHOQW_002034"
     CDS             complement(10500..11495)
                     /gene="wecH"
                     /locus_tag="ACHOQW_002034"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP9573117.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            11670..11969
                     /gene="ysaB"
                     /locus_tag="ACHOQW_002035"
     CDS             11670..11969
                     /gene="ysaB"
                     /locus_tag="ACHOQW_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP9573118.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            12064..12975
                     /gene="glyQ"
                     /locus_tag="ACHOQW_002036"
     CDS             12064..12975
                     /gene="glyQ"
                     /locus_tag="ACHOQW_002036"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9573119.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            12985..15054
                     /gene="glyS"
                     /locus_tag="ACHOQW_002037"
     CDS             12985..15054
                     /gene="glyS"
                     /locus_tag="ACHOQW_002037"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9573120.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            15377..15529
                     /gene="hokA"
                     /locus_tag="ACHOQW_002038"
     CDS             15377..15529
                     /gene="hokA"
                     /locus_tag="ACHOQW_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP9573121.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(15506..15577)
                     /locus_tag="ACHOQW_002039"
     CDS             complement(15506..15577)
                     /locus_tag="ACHOQW_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573122.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(15717..15929)
                     /gene="cspA"
                     /locus_tag="ACHOQW_002040"
     CDS             complement(15717..15929)
                     /gene="cspA"
                     /locus_tag="ACHOQW_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP9573123.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(16210..16500)
                     /gene="yiaG"
                     /locus_tag="ACHOQW_002041"
     CDS             complement(16210..16500)
                     /gene="yiaG"
                     /locus_tag="ACHOQW_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP9573124.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            16934..17644
                     /gene="yiaF"
                     /locus_tag="ACHOQW_002042"
     CDS             16934..17644
                     /gene="yiaF"
                     /locus_tag="ACHOQW_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP9573125.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(17694..18668)
                     /gene="ghrB"
                     /locus_tag="ACHOQW_002043"
     CDS             complement(17694..18668)
                     /gene="ghrB"
                     /locus_tag="ACHOQW_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP9573126.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(18772..19431)
                     /gene="yiaD"
                     /locus_tag="ACHOQW_002044"
     CDS             complement(18772..19431)
                     /gene="yiaD"
                     /locus_tag="ACHOQW_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP9573127.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            19584..21917
                     /gene="bisC"
                     /locus_tag="ACHOQW_002045"
     CDS             19584..21917
                     /gene="bisC"
                     /locus_tag="ACHOQW_002045"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP9573128.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(21886..22326)
                     /gene="yiaC"
                     /locus_tag="ACHOQW_002046"
     CDS             complement(21886..22326)
                     /gene="yiaC"
                     /locus_tag="ACHOQW_002046"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9573129.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(22323..22886)
                     /gene="tag"
                     /locus_tag="ACHOQW_002047"
     CDS             complement(22323..22886)
                     /gene="tag"
                     /locus_tag="ACHOQW_002047"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9573130.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            23044..23742
                     /gene="yhjY"
                     /locus_tag="ACHOQW_002048"
     CDS             23044..23742
                     /gene="yhjY"
                     /locus_tag="ACHOQW_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9573131.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            23971..25173
                     /gene="yhjX"
                     /locus_tag="ACHOQW_002049"
     CDS             23971..25173
                     /gene="yhjX"
                     /locus_tag="ACHOQW_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9573132.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         6266 a         6093 c         6396 g         6450 t
ORIGIN      
        1 tcagacagtt tgccagcgat aagccagaca aaactcatcc ctgagcggta agtccacagg
       61 atgagaagca agggtgagcg atcgcgcgca aaaaacggct gaattttgcg ataaacccca
      121 cattttctgc gatttagcgc caatctgaat cgttaactcg tgacatagtt tcagatttgg
      181 actattcctt ggggtgctct tgacagctat ttttgatatg atttgagatt caactctcaa
      241 atttgtgaaa aatataaggt gttggaatga ttaaatccga ccaggagacc tgatgatttt
      301 gactcccata cgacgatatg gggcgatgat tcttatgtta ctcactctgg tgttttcgag
      361 tgaggtgtta gcgaagacgc acacaacaac agcgagtcaa aagtcccact taactaaagc
      421 tagtaataaa caggtaagca gtaaacaaga gtattctcgc aatagtgcaa agagtagttc
      481 acttcctgat ttgcgaaaat acccttccgg aacatcaagg aaaaaggcgt ttctccggac
      541 cgtaatgcct tacattacca gccaaaatgc agccattact gcagaacgta actggctgat
      601 ttcaaaacag tatcagggcc aatggtcacc tgctgagcgt gcgcgtctga aagacatcgc
      661 caaacgctac aaggtgaagt ggtctggtaa tacgcgaaaa atcccgtgga ataccttgct
      721 tgaacgcgta gacattatcc ctaccagtat ggtggcgacg atggctgcag cagaaagcgg
      781 ttggggaacg tcgaagctgg cgcgcaacaa caacaatctt ttcggcatga aatgcatgaa
      841 aggacgttgt accaatgcgc caggtaaagt gaaagggtac tcacagttta gttctgtcaa
      901 agaatcggtg agcgcctatg tcactaacct caatacgcat ccggcttact cttcgttccg
      961 taaatcgcgt gcgcagctgc gtaaagctga tcaggaagtg actgccacag cgatgattca
     1021 caagctgaag ggctactcga ccaaagggaa gagttataac aactacctgt tcgcaatgta
     1081 ccaggataac caacggttaa tcgcggcgca tatgtaattg tatttcctcg agccttatcc
     1141 gacttgtcgg tcggataagg cttttgatcg catcaggtgg ttgtgctgag ttacctgatg
     1201 cgacgccgac aattctcatc atcgctacaa catgacctcg ctatttacat cgcgatactc
     1261 ttttggcgtc gtgtcatatg cttttttaaa aacagagtag aaatattgca gcgatggata
     1321 accgcacatt tgcgatatct cattgatcga caaggtggtt gaaatcagca gactgcgcgc
     1381 tttctccagc ttctcagcat gaatcatggc atggatggtt tcacccacct cttctttaaa
     1441 acgcttctca agattggagc gcgagatccc gaccgcatcg agtacctgat ccactttaat
     1501 ccctttacag gcgtgattac gaatgtaatg catggcctga ataacggcgg gatcggtcag
     1561 cgaacggtaa tctgttgagc gccgttcaat gacgcgaact ggagggacca aaatccgctg
     1621 tagcggcatt tcttctttat ctaataatcg atgcaacagt tttgccgcct gatagcccat
     1681 ttgccgcgcg ccctgagcga ccgaagaaag ggcgacacgc gacagatagc gggtcagttc
     1741 ttcgttatcg atgccaatca cgcataattt ttccggtacg ggaatgtgta gatgttcaca
     1801 tacttgcaga atatgccgcg cccgggcgtc agtaacggca ataatcccgg tttgcggtgg
     1861 cagtgtttgt agccagtctg ccagccgatt ttgcgcgtgt tgccagttct ctggtgcggt
     1921 ttctaacccc tgataaacca ctccgcgata cttttcttcg gcgacaagct gacgaaatgc
     1981 atattcgcgt tcagtggccc aacgtttgcc gcttgattcc ggaagaccat aaaaagcaaa
     2041 gcggttaacg cctttctctt ttaaatgcaa aaatgcgctt tcaaccagcg catagttatc
     2101 ggtggcaatg taatgaacgg gtgggtaact ttctgcaagg tgatacgagc cgccaacccc
     2161 aacaatgggg acgtcgacat cagccagcgc ttgctcgatc tgtttgtcgt cgaagtcggc
     2221 aatgacgcca tctcctaacc agtccttgat tttatcaatg cgggcgcgga aatcttcttc
     2281 aatgaaaata tcccattccg attgtgacgc ctgtaaatat tcccctacgc cttctactac
     2341 ctgccggtca taggctttat tggcattgaa cagtaatgtg atgcggtgac gtttagtaaa
     2401 catggttctt ttcctgctga atcatgcaaa aactcaaaac cggtaatacg ttaccggctt
     2461 tgagaaaatt tttatcaaaa tcaagaacgg cgtttggttg cggagtccat ccatactgcc
     2521 agcaacagaa tcgcaccttt aacgatatac tgccagaagg tcggtacatc catcatactc
     2581 ataccgttat ccagtgaagc catgatgaat gcccccatta ccgctccagc cacgctcccg
     2641 acgccgcctg ccagactggt gccgccaatc acgcatgctg caattgcgtc cagctcggcg
     2701 atattccccg cagaaggtga accagcgcca agtcgagaac tgaggattaa tccggcgatg
     2761 gctaccatta atccgttaat agcgaacacg gcaagtttgg tgcgttcaac gttaattccg
     2821 gagagacgtg ctgcttccag attgccgccg atggcataaa tgcgtcgtcc aaatgctgtc
     2881 cgcgttgcca taaacattcc gccgagtaac agcaacgtca gcagcagaac aggagtagga
     2941 acgccacggt aatcattcaa cagccagatt gcgcctaata cgatgatagc ggttaaagcc
     3001 tggcgaccga ctactgcggt agaggctgga gactgcaaac ccaaagcctg acggcgcatt
     3061 cttccgcgcc attgccagcc aacaaaagcc attaagccaa gcgcgccaat gatgaagccg
     3121 gtgctggcgg ggagatagct ttgcccaatt tgtgacatcg cggcgctggt gggggataca
     3181 gtcgtgccgt tggtgatgcc aatgagtatg ccgcgaaatg ccaacatgcc cgcgagagtg
     3241 acaataaatg aagggacttt gcggtacgcg acccaccatc cgttccaggc accgagaagc
     3301 agtcccagaa ccaacgtcac aatgatggta agtggcaaag gccagcctag ccagacgtca
     3361 caaatcgccg cgacgccacc taatagcccc atcattgagc cgacggaaag gtcgatttca
     3421 gcagaaatta tgacgaacac cattcctacc gcgaggatgc cggtaatcgc ggtctggcgt
     3481 aacaggttgg agacgttacg ggcgcttaag taggcaccat cggtggtcca ggtaaagaac
     3541 agcatgattg cgatgatagc tgcaatcatc acgaagacct gcaaattcag tgatttcagc
     3601 ccggagaagc caccggatgt cggtacggcc aatttcactt cagacggatt gcttttcgac
     3661 atgatgttcg ctcctcaatg cggcttccat cacctgctcc tgagtcaggt tatgatttat
     3721 caggttggct tttagtttcc cttcatgcat tactagtaca cgatcgctaa ggccgagcac
     3781 ttcaggtaat tcagaagaga tgacaataac ggcaataccc tgctggacga gttggttaat
     3841 taatttgtag atctcgtatt tcgcgccaat atcgataccc ctggtgggtt catcaagaat
     3901 gagaatgcgc gggttaagta acagacagcg agcgaggatc gctttttgct gattgccgcc
     3961 gctcaaacgt ccaatagcaa ggtcggggga cgacgtttta actttgagtt gctggattga
     4021 ttccagaata catttttgct ctgccgcgtc atcaagctgg ctaatgccac cggtaaattt
     4081 attgagtgcg gcgagggtaa tatttttacc aaccgccatt accggaacga tgccgtcgcg
     4141 ctttctgtct tcggggacca tcgcaatccc ctgggcgatg gcttgctgac agttacgaat
     4201 atctacctgt ttgccatcaa tataaatttt tccttcccat tgtccgggcc acacaccaaa
     4261 caggcactga atggtctcgg tacgtccggc accaacgagt ccggcaatac ccagtatttc
     4321 gccacgtttc agggaaaacg agacatcatt aactcgttta atatgacgat taaccggatg
     4381 ccatgccgtc agatgttcaa tacgtaatat ttcatctccg gtggtatgtg gttcattagg
     4441 gtaaagcgcg gttaactctc gcccgaccat catggtgata atatcgtctt cactcattcc
     4501 ggcagcatca cgcgtaccaa tgtgctgtcc gtcgcgaata acgcaaatcg tatcggaaat
     4561 cgctttgact tcgttgagtt tgtgcgaaat ataaatacag gcgataccgt gctgttgtag
     4621 atcgcgaata atatccagta aaaccgacgt ttcctgctca gttaatgagg ctgtcggttc
     4681 atcgagaatt aacaagcgca cctgtttatt aagtgccttg gcaatttcaa ccagttgttg
     4741 ttgcccaagc cctaaatcgc caacgcgggt atcaggtgaa atggataaac tgacctgtgc
     4801 gagcagcttc tgacagcgta gcgtcatcag gtcataatcc ataatgccat tgtgggttat
     4861 ttcgttaccc aggaagatat tttccagcac ggtcaattct ttcaccaggg ccaattcctg
     4921 gtgaatgatg gcgatacctt tgcgttcggt atcgcggatg tgactcgcct gaatctcttc
     4981 tcccgcaaaa ataatttcgc cttcgtagga gccatgggga taaataccac acagcacttt
     5041 catcagcgtt gatttaccag acccattttc cccacagagt gagacgattt cgccagcatt
     5101 caaccgcaag ctgacgttat caatcgcctt cacactgccg aaggttttgg taatgttctt
     5161 catttcaagt agataaggca tagcgactcc acctaagcca attcattcac gcggcatgga
     5221 gagaaatcac gcccccgctc cgcgctgggg cgtaacgctt acagctcgct ctctttgtgg
     5281 aatccgtctt taattaccgt atctttgatg ttgtttttat tcacatcgat cggtgtcagg
     5341 aggcgggagg ggacatcttt caggccatta tttagtgaag tatctgcttt tggctcctga
     5401 ccattgccca attcaacggc aatttctgcg gcagtatttg ccagcaatgt aataggttta
     5461 tataccgtca tagtttgcgt accggcagca atacgtttaa tacctgcgag atccgcatcc
     5521 tggccagaaa ttgctacttt ccctgataaa ccttgcgcgc ttaatgcctg aattgcccca
     5581 cctgcggtgg catcgtttga ggcaactaca gcatcaattt tgttattatt ggcggttagc
     5641 gcgttttcca taattttcaa tgcgttttcc ggtaaccagc catcaaccca ttggtcacca
     5701 acgactttaa tttttccgga atcaacgtaa ggttgtaaca ctttcatttg tccggcgcgg
     5761 aacagcttag cgttgttatc taccggcgag ccgcccatca ggaagtaatt accttgcgga
     5821 acaatatcga ccagggcttt tgcctgcagt tcaccgactt tttcgttatc gaaagaaata
     5881 taaaaatcga tatccgcatc gttaatcata cggtcgtaag ctaatacttt aatgccttct
     5941 tgtttggctt cttttacaac gttacttaat acctgaccgt tatacggaat aatgacaaga
     6001 acatcgacac cccggtttat catgttttca atctgcgaca tttgtgtttc ttcattgcca
     6061 tttgcagact gtacaaatac tttcgcgccg agagattctg cctttttcac aaagatatct
     6121 cgatcttttt gccagcgttc aagacggaga tcatcaatcg ccatacctat tttgacttct
     6181 ttggcgtgtg cagcaacgtt ggtaagcagg agtgaggtgc aaagggtgag tagaatgttc
     6241 tttattttca tggtgtaggg ccttctgtag ttagaggaca gctttaataa gtaacaatca
     6301 ccgcgataaa cgtaaccaat ttttagcaac taaacagggg aaaacaatta cagattttta
     6361 tctttcgatt acgatttttg gtttatttct tgatttatga ccgagatctt acttttgttg
     6421 cgcaattgta cttattgcat ttttctcttc gaggaattac ccagtttcat cattccattt
     6481 tattttgcga gcgagcgcac acttgtgaat tatctcaata gcagtgtgaa ataacataat
     6541 tgagcaactg aaagggagtg cccaatatta cgacatcatc catcacccgc ggcattacct
     6601 gattatggag ttcaatatgc aagcctattt tgaccagctc gatcgcgttc gttatgaagg
     6661 ctcaaaatcc tcaaacccgt tagcattccg tcactacaat cccgacgaac tggtgttggg
     6721 caagcgtatg gaagagcact tgcgatttgc cgcctgctac tggcacacct tctgctggaa
     6781 cggggcggat atgtttggtg tgggggcgtt taatcgtccg tggcagcagc ctggtgaggc
     6841 actggcgttg gcgaagcgta aagcagatgt cgcatttgag tttttccaca agttacatgt
     6901 gccattttat tgcttccacg atgtggatgt ttctcctgag ggcgcgtcgt taaaagagta
     6961 catcaataat tttgcgcaaa tggttgatgt tctggcaggc aagcaagaag agagcggcgt
     7021 gaagctgctg tggggaaccg ctaactgctt tacaaaccct cgctatggcg cgggtgcggc
     7081 gacgaaccca gatccagaag tcttcagctg ggcggcaacg caagttgtta cagcgatgga
     7141 agcaacccat aaattgggtg gtgaaaacta tgtcctgtgg ggcggtcgtg aaggttacga
     7201 aacgctgtta aataccgatt tgcgtcagga gcgtgaacaa ctgggccgct ttatgcaaat
     7261 ggtggttgag cataaacata aaatcggctt ccagggcacg ttgcttatcg aaccgaaacc
     7321 gcaagaaccg actaaacatc aatatgatta cgatgctgcg acagtctatg gcttcctgaa
     7381 acagtttggt ctggaaaaag agattaaact gaacattgaa gctaaccacg cgacgctggc
     7441 aggtcactct ttccatcatg aaatagccac cgccattgcg cttggcctgt tcggttctgt
     7501 cgacgccaac cgtggcgatg cgcaactggg ctgggacacc gaccagttcc cgaacagtgt
     7561 ggaagagaat gcgctggtga tgtatgaaat tctcaaagca ggcggtttca ccaccggtgg
     7621 tctgaacttc gatgccaaag tacgtcgtca aagtactgat aaatatgatc tgttttacgg
     7681 tcatatcggc gcgatggata cgatggcact ggcgctgaaa attgcagcgt gcatgattga
     7741 agatggcgag ctggataaac gcatcgcgca gcgttattcc ggctggaata gcgaattggg
     7801 ccagcaaatc ctgaaaggcc aaatgtcact ggcagattta gccaaatatg ctcaggaaca
     7861 taatttgtct ccggtgcatc agagtggtcg ccaggagcaa ctggaaaatc tggtaaatca
     7921 ttatctgttc gacaaataac ggctaactgt gcagtccgtt ggcccggtta tcggtagcga
     7981 taccgggcat ttttttaagg aacgatcgat atgtatatcg ggatagatct tggcacctcg
     8041 ggcgtaaaag ttattttgct caacgagcag ggtgaggtgg ttgcttcgca aacggaaaag
     8101 ctgaccgttt cgcgcccgca tccactctgg tcggaacaag acccggaaca gtggtggcag
     8161 gcaactgatc gcgcaatgaa agctctgggc gatcagcatt ctctgcagga cgttaaagca
     8221 ttgggtattg ccggccagat gcacggagca accttactgg atgctcaaca acgggtattg
     8281 cgccctgcca ttttgtggaa cgacgggcgc tgtgcgcaag agtgcgcttt gctggaagcg
     8341 cgagttccgc tatcgcgggt gattaccggc aacctgatga tgcccggatt tactgcgcct
     8401 aaattgctat gggttcagcg gcatgagccg gagatattcc gtcaaatcga caaagtatta
     8461 ttaccgaaag attacttgcg tctgcgtatg acgggggagt ttgccagcga tatgtctgac
     8521 gcagctggca ccatgtggct ggatgtcgca aagcgtgact ggagtgacgt catgctgcag
     8581 gcttgcgact tatctcgtga ccagatgccc gcattatacg aaggcagcga aattactggt
     8641 gctttgttac ctgaagttgc gaaagcgtgg ggtatggcga cggtgccagt tgtcgcaggc
     8701 ggtggcgaca atgcagctgg tgcagttggt gtgggaatgg ttgatgctaa tcaggcaatg
     8761 ttatcgctgg ggacgtcggg ggtctatttt gctgtcagcg aagggttctt aagcaagcca
     8821 gaaagcgccg tacacagctt ttgccatgcg ctaccgcagc gttggcattt aatgtctgta
     8881 atgctgagtg cagcgtcgtg tctggattgg gccgcgaaat taaccggcct gagcaatgtc
     8941 ccagctttaa tcgctgcagc tcaacaggct gatgaaagtg ccgagccagt ttggtttctg
     9001 ccttatcttt ccggcgagcg tacaccacac aataatcccc aggcgaaagg ggttttcttt
     9061 ggtttgactc atcaacatgg ccccaatgaa ctggcgcgag cagtgctgga aggcgtgggt
     9121 tatgcgctgg cagatggcat ggatgtcgtg catgcctgcg gcattaaacc gcaaagtgtt
     9181 acgttgattg gtggcggggc gcgtagtgag tactggcgtc agatgctggc ggatatcagc
     9241 ggtcagcagc tcgattaccg tacggggggg gatgtggggc cagcactggg cgcagcaagg
     9301 ctggcgcaga ttgcggcgaa tccagagaaa tcgctcattg agttgttgcc gcaactaccg
     9361 ttagaacagt cgcatctacc agatgcgcag cgttatgccg cttatcagcc acgacgtgaa
     9421 acattccgtc gcctctatca gcaacttctg ccattaatgg cgtaaacgtt atcccctgcc
     9481 tgaccgggtg ggggataatt cacatctata tatctcagca attaattaat atttagtatg
     9541 aatttattct gaaaatcatt tgttaatggc atttttcagt tttgtctttc gttggttact
     9601 cgtaatgtat cgctggtaga tatggagatc gttatgaaaa cctcaaaaac tgtggcaaaa
     9661 ctattatttg ttgtcggggc gctggtttat ctggttgggc tatggatctc atgcccattg
     9721 ttgagtggaa aaggctattt tcttggcgtg ttaatgacag caacttttgg caactatgca
     9781 tatcttcgcg cagaaaaact cgggcaactg gataattttt ttacccatat ttgccagtta
     9841 gttgcgttaa tcactatcgg tctcttgttt atcggtgttt taaatgcacc tatcaatgct
     9901 tatgaaatgg tgatctaccc catcgccttt tttgtctgct tgtttggtca aatgcgtttg
     9961 tttcgctcgg tatgagcaac ataaagctct tacacattca ggaatgaaag gaatactgtg
    10021 atggacaaca aaatatcaac ctattcaccg gcctttagta ttgtgtcatg gatagctctc
    10081 gttggtggta tcgttaccta tctgttaggg ctatggaatg cagagatgca gttaaatgaa
    10141 aaaggatatt attttgccgt actggtatta ggactgtttt ctgcggcgtc ttatcaaaag
    10201 accgttcggg acaagtatga aggcataccg accacttcca tttattatat gacctgcctg
    10261 actgtcttta ttatctctgt tgcattactg atggtaggtc tgtggaatgc gacattatta
    10321 ctcagcgaaa aaggttttta tggactggct ttcttcttaa gcttgtttgg tgcagtagcg
    10381 gtgcagagaa atattcgtga tgccggaata aacccaccaa aagaaacaca ggttacccag
    10441 gaagaataca gcgaataact cacgtaagcc cggtcagtcc aatgtgaccg ggcttttact
    10501 taactcacta atctgtttct gtcgattcgt tgtaccagca tagaaagtaa caaactcgct
    10561 gccaacgtcg cgcaaaaaat ccaaataata tccagtattg gccaattttt aagctcaatt
    10621 ccccgggtgc gcagcgcatg gataatcaag gcgtggaatc cgtatatacc caacgaatgg
    10681 cgggagatta agccaagtcc gcgaatggta cgcgtatcca gcgtgttttt aaccagagtc
    10741 aatagcgcga ttgcgcagat aaaaaccatc ggcccacagt aaggatacca ggtatcggca
    10801 aaatttccgc gccactgcaa ttcatataat gtcccgcgag agataataaa aactcccgtc
    10861 gcaaacagtg cggcgctcac ccacgacagt gctttatgct gtgtgtccat catccctaaa
    10921 gcgcggccca acatgccata cagaatgtag taaaaagtat cgccattgat atataagtta
    10981 attggcagcc attcaaaacc gtcgattttc tgcggcacgg tgtttgggtt agcgatgata
    11041 ccaatcacca ccattagcac cagcaacatt tttccgccga cgttcttcac ctgaatcagc
    11101 ggtgaaacca gataaatcac cgcaatcgcg aagaaaaacc ataagtggta aaacactggc
    11161 ttttgcagca ggtttttcag cgctaactcc acattaatgg aggtaaacag tgcaatgtag
    11221 agcagcgcga ttgcgctata aaaaaacaga cataagccga tacgcaagaa atggcgcggc
    11281 tgggcgctgc gttcgccaaa aaagagatag ccggaaatca tgaaaaatag cggcacgctg
    11341 acacgagagg cagagttcag aacattggcg atatcccagg tgacggggct aacactatga
    11401 gcattggtca cataccaggt agtggtgtga atcatcacca ccattaaaca cgctatccct
    11461 cgcaggttat caatccagta aattttgggc tgcatctgtg tctctgtatc tggttaaaaa
    11521 aagtctgacc gataaatcat tcagttggcg cactggaata atctgagttt taacactaca
    11581 gcttatagag gcttaaggaa attcgtaaga tatcagccac tataccgata taaataataa
    11641 gactcacctg caaaccagac ggtaatttaa tgatgatgaa ctctttcttt ccggcaatgg
    11701 cgcttatggt gctagtgggt tgttctacac cgtcacccgt gcagaaagca caacgggtaa
    11761 aggttgatcc tctgcgttcg ttgaatatgg aagcgttatg caaggatcag gcggcaaaac
    11821 gttataacac cggcgagcaa aaaatcgacg tcaccgcctt cgaacagttc cagggaagct
    11881 atgaaatgcg cggttatacc ttccgtaaag agcagtttgt ctgttctttt gacgcagatg
    11941 gccatttttt gcatctttcc atgcgttaag ccctgctttt tcccgtttca tactgtatat
    12001 cttccatcca gcgggtatac tgatcccttc ctttaaatcc acacgtatcc agcacgaaat
    12061 aatatgcaaa agtttgatac caggaccttc cagggcttga tcctgacctt acaggattac
    12121 tgggctcgcc agggctgcac cattgttcaa ccattggaca tggaagtcgg cgcgggaacc
    12181 tctcacccaa tgacctgtct gcgcgcgctg gggccagaac cgatggcggc tgcttatgtt
    12241 cagccttctc gtcgcccgac cgatggtcgc tacggcgaaa accccaaccg tttacagcac
    12301 tactatcagt tccaggtggt cattaagcca tcgccggaca atattcagga gctgtacctt
    12361 ggttctctga aagagctggg catggacccg actatccacg atatccgttt cgtggaagat
    12421 aactgggaaa acccgacgct gggtgcctgg ggactgggct gggaagtgtg gctaaacggc
    12481 atggaagtga cgcagttcac ttacttccag caggttggtg gtctggagtg taaaccggtt
    12541 accggcgaga tcacctacgg tctggaacgt ctggccatgt acattcaggg cgtagacagc
    12601 gtttacgacc tggtctggag cgacggcccg ctgggtaaaa ccacctacgg cgacgtgttc
    12661 catcagaacg aagtggagca gtccacttac aacttcgaat acgcggatgt ggacttcctg
    12721 ttcacctgct ttgagcagta cgagaaagaa gcgcagcagc tgctggcgct ggaaaatccg
    12781 ctgccgctgc cagcttacga gcgtattctg aaagccgccc acagcttcaa cctgctggat
    12841 gcgcgtaaag ccatctccgt caccgagcgt cagcgctaca ttctgcgcat tcgcaccctg
    12901 accaaagcag tggcagaagc atattacgct tcccgtgaag ccctaggctt cccgatgtgc
    12961 aacaaagata agtaagaggc ggctatgtct gagaaaactt ttctggtgga aatcggcact
    13021 gaagagctgc caccaaaagc actgcgcagc ctggcagagt cctttgctgc gaactttact
    13081 gcggagctgg ataacgcagg cctcgcacac ggcaccgttc aatggtttgc tgctccgcgt
    13141 cgtctggcgc tgaaagtagc taacctggcg gaagcgcaac cggatcgtga aatcgaaaaa
    13201 cgcggcccgg cgattgccca ggcgttcgac gctgaaggca aaccgagcaa agcggcagaa
    13261 ggttgggcgc gtggttgcgg tattaccgtt gaccaggctg agcgtctgac taccgataaa
    13321 ggcgaatggc tgctgtatcg cgcccatgtg aagggcgaaa gcactgaagc actgctgccg
    13381 aatatggttg cgacttcgct ggcgaaactg ccgattccga aactgatgcg ctggggtgca
    13441 agcgacgtgc acttcgtgcg tccggtgcac accgtgaccc tgctgctggg cgacaaagtc
    13501 attcctgcaa ccattctggg cattcagtcc gatcgcgtga ttcgcggcca ccgctttatg
    13561 ggcgagccgg aattcaccat cgataacgcc gatcagtatc cggaaattct gcgtgagcgt
    13621 gggaaagtca tcgccgatta cgaagaacgt aaggcgaaga ttaaagccga tgccgaagaa
    13681 gcagcgcgta agattggcgg taacgctgac ttaagcgaaa gcctgctgga agaagtggct
    13741 tcgctggtgg agtggccggt cgttctaacc gcgaaatttg aagagaaatt cctcgcggtg
    13801 ccgtctgaag cactggttta caccatgaaa ggtgaccaga agtacttccc ggtgtatgcg
    13861 aacgacggca aactgctgcc gaactttatc ttcgttgcca acatcgaatc gaaagatccg
    13921 cagcaaatta tctctggtaa cgagaaagtc gttcgtccac gtctggctga tgccgagttc
    13981 ttcttcaaca ccgaccgtaa aaaacgtctg gaagataacc tgccgcgcct gcaaaccgtg
    14041 ctgttccagc aacagctggg tacgctgcgt gacaagactg accgcatcca ggcgctggct
    14101 ggctggatcg ccgaacagat tggcgctgac gttaaccacg caacccgtgc gggcctgctg
    14161 tctaagtgtg acctgatgac caacatggtc ttcgagttca ccgacaccca aggcgttatg
    14221 gggatgcact acgcgcgtca cgatggcgaa gcggaagacg tagccgtcgc gctgaatgag
    14281 cagtatcagc cgcgctttgc cggtgatgac ctgccgtcca acccggtagc ttgtgcgctg
    14341 gcgattgctg ataagatgga tactctggcg ggtatcttcg gtatcggtca gcatccgaaa
    14401 ggcgacaaag acccgtttgc gctgcgtcgt gccgcacttg gcgtgctgcg aattatcgtt
    14461 gagaagaacc tcaaccttga tctgcaaacg ctgaccgaag aagcggtgcg tctgtatggc
    14521 gataagctga ctaatgccaa cgtagttgat gatgttatcg actttatgct cggtcgcttc
    14581 cgcgcctggt atcaggacga aggttatacc gttgacacca tccaggcggt actggcgcgt
    14641 cgtccgactc gtccggctga tttcgatgcc cgcatgaaag cggtatcgca cttccgtacc
    14701 ctggaagcag ctgcagcact ggcggcggcg aacaaacgtg tatctaacat cctggcgaaa
    14761 tctgacgaag tgctgagcga tcgcgtgaat gcctctactc tgaaagagcc ggaagaaatt
    14821 aaactggcga tgcaggttgt ggtgctacgt gacaagctgg agccgtactt tgcggaaggt
    14881 cgttaccagg atgcgctggt cgaactggca gagctgcgtg aaccagtcga tgccttcttc
    14941 gataaagtga tggtcatggt tgatgacaaa gaattgcgta tcaaccgtct gaccatgctg
    15001 gagaaactgc gcgaactgtt cctgcgcgtt gcggatattt cgctgttgca gtaataacgc
    15061 cgttattaaa aagcctgcca tctggcaggc tttttttatc gctaaataat acagcaacct
    15121 ttaataatct tctgctgaat aaagattatc tcatatatta attttatgag atttttttag
    15181 gattatatca aggagaagaa acaaacttat taagctagaa tagccacggg tgcttgagac
    15241 tgtttgtctc aggtattcac cgaaaggcag acagagaaaa gccccacctg actataaatc
    15301 aaagtgaggc ccctctatgt atagacacca taaggttagc ctcttactcg ttggaaatca
    15361 acacaggggg ctgggaatgc cgcagaaata tagattgctt tctttaatag tgatttgttt
    15421 cacgctttta tttttcacct ggatgataag agattcactg tgtgaattgc atattaaaca
    15481 ggggagttat gagctggcgg catttttagc ctgcaatttg aaagagtaag agtcttcggc
    15541 ggaaagttat tcccgccttt cttacggcgt tgcgcattct cattgcaccc aaatttattt
    15601 ttcacaaaaa taatactaga ttttattacg cgatcgatta tttatttcct gaaaaacaaa
    15661 taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac
    15721 aggctggtta cgttgccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact
    15781 ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac
    15841 acgtctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat
    15901 tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct
    15961 ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg
    16021 gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt
    16081 caaaccgttg atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata
    16141 tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg
    16201 gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
    16261 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
    16321 gactgatacg cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcgtaa
    16381 ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc
    16441 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
    16501 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
    16561 tcgggaaaag cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca
    16621 gccagtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
    16681 aaccactcct ggtttgtaaa gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct
    16741 ggctacaaaa aaggcaacgg caatatcatt ttttaacaat gaaacaacaa attaaccttg
    16801 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
    16861 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
    16921 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
    16981 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
    17041 gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac
    17101 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
    17161 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggttgtag acagcgttaa
    17221 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg
    17281 ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca
    17341 ctctgcgcta aaacagagtg atgacctgaa accggtcttt gatcaggcat tcaccaaagt
    17401 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca
    17461 acaactggtg atggttggag actacattgc ccaacaaggt actcaggtga gctttgtagc
    17521 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
    17581 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
    17641 ataaagagta aaagcccgag cgattatcgg gctttactcc acgcagtcgc ggcttagtcc
    17701 gcgacgtgcg gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta
    17761 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
    17821 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg
    17881 aagacatcca gcccggcggc gtgaatttcc cctttctgca atgctgcgat cagtgcattt
    17941 tcgtcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
    18001 aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg
    18061 aaatctgact cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
    18121 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcgca
    18181 cgttgtgcca gcgccatacc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
    18241 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
    18301 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
    18361 acggtttctg ttaagacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
    18421 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt
    18481 ttttccagca atgcggcatc aacattctcg tttgaaccca gtaaaccttc agcttcggca
    18541 aaaattgctg cattttgttc gacggtttgt gggctgaggt ttgccacctg gtgaacggtg
    18601 aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
    18661 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
    18721 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
    18781 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc
    18841 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
    18901 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcagatcgtg
    18961 accacccgtg ctgtcagtat aaccaatcac gttaaccgcc gttttcggat actctttcag
    19021 caccattgcc acgccggtca gggtgttagc gcccgccggt ttcagggttg cgctgctgct
    19081 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
    19141 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
    19201 cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc
    19261 tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc
    19321 gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac
    19381 cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc
    19441 ttaataacct ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca
    19501 tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat
    19561 ctaacctttc aggagtcttc ggtttgacca actcatcctc acgatattcc gttctgactg
    19621 ccgcccattg ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg
    19681 gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgtgac caggttcaca
    19741 gcaatacgcg ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc
    19801 cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc
    19861 ttattcacca acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg
    19921 gttcctacgg ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct
    19981 atatggcgct ggcaggcggt tataccgggc atctggggga ttattcgacc ggcgcggcac
    20041 aggcgatcat gccgtatgtc gtgggtggta gtgaagttta tcaacagcag accagttggc
    20101 cgctggtgct ggaacatagc gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc
    20161 tgaaaattgc gtggaatgca tccgatgagc aggggctttc ttacttttct gcactgcgtg
    20221 acagcgggaa aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg
    20281 gcgataaaat ggagtgggtg gcaccgcaca tgggcactga tgttgcgctg atgctgggga
    20341 tcgcccatac gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca
    20401 ccggttatgc cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaccg
    20461 ccgaatgggc agcagagatt tgtggtgtta acgcagagaa aatccgcgag ctggcggcta
    20521 ttttccacca aaataccacc atgctgatgg ctggctgggg aatgcagcgc caacagtttg
    20581 gtgagcaaaa acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac
    20641 ccggcggcgg ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcggcgcg
    20701 ctgcggtact ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtagataaaa
    20761 tccctgtcgc ccgcattgtt gaagcactgg aaaaccccgg tggcgcatat caacacaacg
    20821 gtatggaccg acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta
    20881 ctcatcatca ggataccaat cgcctgatcc gtgcctggca aaaaccggag ctggtggtga
    20941 tctctgaatg cttctggacg gcggcggcaa aacacgcgga tatcgttctg cctgcgacta
    21001 cctcttttga gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc
    21061 cgatgaagca agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg
    21121 agttaagtga acgctgggag aagggcggtt atgcacgttt tacggaagga aaaagtgagc
    21181 tgcaatggct ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg
    21241 aattgccgcc atttactgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc
    21301 cggacagcga gcggtttatt cgcttcgctg atttttgccg cgatccgcag gcgcatccat
    21361 taaaaaccgc cagcggtaag attgaaatct tctcgcagcg tattgccgat tacgcttatc
    21421 cggattgccc ggggcatcca atgtggctgg tgccggacga atggcagggc aatgccgaac
    21481 cggaacagtt acaggtactt tctgctcatc cggcgcatcg tctgcacagc cagctgaatt
    21541 acagttctct gcgcgaattg tacgcggtgg caaaccgtga gcctgtcacc attcatcctg
    21601 acgatgccca ggcgcgcggc atacaagatg gcgatactgt tcggctgtgg aacgcacgcg
    21661 ggcaaattct tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc
    21721 acgaaggggc atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag
    21781 tgaacgtgct gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata
    21841 cggcgctggc atggctagaa aaatacaacg gtccggaact gacacttaca gcgtttgaac
    21901 caccggccag ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac
    21961 aatgtgaaaa ccctgcgcct ggtaaaaatc tatcgccggt tgattttttt gataaacctc
    22021 cagcatcaga tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg
    22081 tctgacggcc ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac
    22141 aaaaccgaga agcttaccgt cttcttccca gacccagttt ttcgcattgg caagatacgc
    22201 atcccgcacc agcggaatgc actcgcgcca gtaattcact tttataaagg gatgccccca
    22261 ggttgtactt tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg
    22321 aatcatggtt tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca
    22381 catgcctgca taaaggagta acagattgtg gtaccgacaa acttaaaacc acgttttttc
    22441 agtgccttag atagggcgtc ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg
    22501 gcttgtgtca cctgtggctg atgatttaca aacgaccaga caaagtcggc aaacggttcg
    22561 ccgttctgtt ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttcccg
    22621 cgatggcgga taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgcg
    22681 gcaaccttca ccggatcgaa ctgatgaaag caggcgcgat agttttcgcg ttttttgagg
    22741 acggtgatcc acgataaccc agcctgctgc ccttccaggc agatcatttc gaacagtttt
    22801 ttcctgtcag tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc
    22861 tgactcaccc agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt
    22921 tcagccgctt ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc
    22981 ccgatatcct tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg
    23041 ttaatgatca taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagtgtggta
    23101 atcagtgcct tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg
    23161 caacccgggc attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac
    23221 gatattttgt cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg
    23281 gcggaagaaa cccctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta
    23341 tatgaacagg ttacaactgg cccggtcgcg gcattacatt acgatggcac aaccacctcg
    23401 atgtataacg agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc
    23461 gtgagtacct taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa
    23521 atcagctata accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg
    23581 atgacggcga caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg
    23641 ctcaatcaaa atattgccgc ctatgccgcg ctatctcagg cagaaaatac cactaacaat
    23701 agcgattatc tgtatacgat gggggttagt gccagatttt aacgtaacaa tcacaattga
    23761 aaccattgaa taacaatagt tgtggcgata gtgggtgcta acttaccaaa taataaattt
    23821 ggtgaataat tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc
    23881 atttcatgcc gtttttcccc aggcataaag tgcacttcgt tatggttgtc ggcagagatt
    23941 tttccttttt attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc
    24001 tggttgacac tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg
    24061 agcctgttta acggcgcgct ttccgccaag ctggatgcgc cggtaagcca ggtcgctttc
    24121 tctttcggct tgttaagtct ggggctggca atatcgtctt ctgttgcggg caaattgcag
    24181 gaacgttttg gtgttaaacg cgtcaccatc gcttccggca ttttgctggg attagggttc
    24241 ttcctgacag cgcattctga caacctgatg atgctgtggt taagcgccgg tgtgctggtc
    24301 ggtctggcgg atggcgcggg ttacctgctg acgctctcta actgcgtgaa atggttcccg
    24361 gagcgtaaag gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt
    24421 ttcaaattta tcgacacgca gctgctggaa acggtcggtc tggaaaaaac ctttgtgatt
    24481 tggggagcga ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca
    24541 aaacaggaag tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg
    24601 atgcgtaaac cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc
    24661 ctgtacgtga ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt
    24721 tctgcagcca atgcagtcac tgttatttcc atcgccaacc tttcaggtcg tctggtgctg
    24781 ggcattctgt ctgacaaaat cgcccgtatc cgtgttatca ccattggtca ggtaatttcg
    24841 ctggtgggta tggcggccct gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg
    24901 attgcctgcg tggcatttaa ctttggcggc actattaccg tctttccgtc actggtcagt
    24961 gagttcttcg gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt
    25021 atcggtagca tttgtggttc gattatcgcc tcactgtttg gcggcttcta tgtgaagttc
    25081 tacgtcatct ttgctctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca
    25141 gagcagaaag tattgcgtga ggcgcatatt tagccagaaa ctacaggttt tgaacgaaag
    25201 gggga
//
LOCUS       DAZKFZ010000056        24503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000056 DAZKFZ010000000
VERSION     DAZKFZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..134
                     /locus_tag="ACHOQW_002050"
     CDS             <1..134
                     /locus_tag="ACHOQW_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFP9573133.1"
                     /translation="DGATHLYDEVIIINKITPKKIDTEEVATKQSTAEQITDNAIIE"
     gene            complement(161..339)
                     /locus_tag="ACHOQW_002051"
                     /pseudo
     CDS             complement(161..339)
                     /locus_tag="ACHOQW_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(552..1211)
                     /gene="rluA"
                     /locus_tag="ACHOQW_002052"
     CDS             complement(552..1211)
                     /gene="rluA"
                     /locus_tag="ACHOQW_002052"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP9573134.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1223..4129)
                     /gene="rapA"
                     /locus_tag="ACHOQW_002053"
     CDS             complement(1223..4129)
                     /gene="rapA"
                     /locus_tag="ACHOQW_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP9573135.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4293..6644)
                     /gene="polB"
                     /locus_tag="ACHOQW_002054"
     CDS             complement(4293..6644)
                     /gene="polB"
                     /locus_tag="ACHOQW_002054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP9573136.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(6719..7414)
                     /gene="araD"
                     /locus_tag="ACHOQW_002055"
     CDS             complement(6719..7414)
                     /gene="araD"
                     /locus_tag="ACHOQW_002055"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9573137.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(7614..9116)
                     /gene="araA"
                     /locus_tag="ACHOQW_002056"
     CDS             complement(7614..9116)
                     /gene="araA"
                     /locus_tag="ACHOQW_002056"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9573138.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(9127..10827)
                     /gene="araB"
                     /locus_tag="ACHOQW_002057"
     CDS             complement(9127..10827)
                     /gene="araB"
                     /locus_tag="ACHOQW_002057"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP9573139.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRSEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            11166..12044
                     /gene="araC"
                     /locus_tag="ACHOQW_002058"
     CDS             11166..12044
                     /gene="araC"
                     /locus_tag="ACHOQW_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP9573140.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12130..12894
                     /gene="yabI"
                     /locus_tag="ACHOQW_002059"
     CDS             12130..12894
                     /gene="yabI"
                     /locus_tag="ACHOQW_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9573141.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12981..13679)
                     /gene="thiQ"
                     /locus_tag="ACHOQW_002060"
     CDS             complement(12981..13679)
                     /gene="thiQ"
                     /locus_tag="ACHOQW_002060"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP9573142.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(13663..15273)
                     /gene="thiP"
                     /locus_tag="ACHOQW_002061"
     CDS             complement(13663..15273)
                     /gene="thiP"
                     /locus_tag="ACHOQW_002061"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP9573143.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            complement(15249..16232)
                     /gene="thiB"
                     /locus_tag="ACHOQW_002062"
     CDS             complement(15249..16232)
                     /gene="thiB"
                     /locus_tag="ACHOQW_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP9573144.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(16263..16362)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16604..17173
                     /gene="espY2"
                     /locus_tag="ACHOQW_002063"
     CDS             16604..17173
                     /gene="espY2"
                     /locus_tag="ACHOQW_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFP9573145.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     gene            complement(17402..19060)
                     /gene="sgrR"
                     /locus_tag="ACHOQW_002064"
     CDS             complement(17402..19060)
                     /gene="sgrR"
                     /locus_tag="ACHOQW_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP9573146.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            19149..19280
                     /gene="sgrT"
                     /locus_tag="ACHOQW_002065"
     CDS             19149..19280
                     /gene="sgrT"
                     /locus_tag="ACHOQW_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP9573147.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(19388..19993)
                     /gene="leuD"
                     /locus_tag="ACHOQW_002066"
     CDS             complement(19388..19993)
                     /gene="leuD"
                     /locus_tag="ACHOQW_002066"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP9573148.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(20004..21404)
                     /gene="leuC"
                     /locus_tag="ACHOQW_002067"
     CDS             complement(20004..21404)
                     /gene="leuC"
                     /locus_tag="ACHOQW_002067"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP9573149.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21407..22498)
                     /gene="leuB"
                     /locus_tag="ACHOQW_002068"
     CDS             complement(21407..22498)
                     /gene="leuB"
                     /locus_tag="ACHOQW_002068"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP9573150.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(22498..24069)
                     /gene="leuA"
                     /locus_tag="ACHOQW_002069"
     CDS             complement(22498..24069)
                     /gene="leuA"
                     /locus_tag="ACHOQW_002069"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP9573151.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(24162..24251)
                     /gene="leuL"
                     /locus_tag="ACHOQW_002070"
     CDS             complement(24162..24251)
                     /gene="leuL"
                     /locus_tag="ACHOQW_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP9573152.1"
                     /translation="MTHIVRFIGLLLLLNASSLRGRRVSGIQH"
BASE COUNT         5548 a         6602 c         6607 g         5746 t
ORIGIN      
        1 tggacggtgc tacccaccta tatgatgagg taattattat taataaaatc acccccaaaa
       61 aaattgatac tgaagaagtt gctactaaac aaagtactgc tgaacaaatt actgacaacg
      121 caattattga atgagccaga ataagctaag gttgaagggg ctggaacgcc ccttcaacct
      181 tagcagtagc gtgggatgat ttcacaatta gaaagacctg catgatgagc tagagaagag
      241 gctagtgacg caaggcgtcg tgtacgacgc ggatcaccga gatgggcatc gccaaccaga
      301 ctgctaatta gcccatgaat aacaatcaga aaggaccata acagacccga taaaatgaaa
      361 tataagagac ggtcaacggg tgaagaaaaa gttcaaaaat tcgctgtgga gcaggaaggg
      421 aattaccgaa tggaaagcgt agccacacgc aacaactgaa agcagtttgg cagaaacaaa
      481 aaatccccgg actcggggat ttatgtacaa gaggcagccc ttaggatgag ggtataaacg
      541 tacaggaaag gttaaaaatc cgctggcgct ttaaacgtca tactattgcc atacgccgga
      601 tgggtaatcg tcaacatctc tgcatgtagc aacaaacgcg gtgccatcgc tctcgcttct
      661 ggtgatgcat aaaaacgatc gccgagaatc ggatgcccca gcgccagcat atgcacacgc
      721 aattgatgcg aacgcccggt aatcggtttt aacaccactc ttgccgtgtt atccgccgca
      781 tactccacca cttcatattc cgtctgcgca ggtttacccg tttcgtaaca gactttctgt
      841 ttcgggcggt ttggccagtc gcaaatcagc ggcagatcca ccagaccttc tgcgggggat
      901 ggatgccccc agacgcgggt cacatactgc tttttcggca cgcgctcgcg gaactggcgt
      961 tttaactccc gctccgcggc tttggtcagc gccactacaa tcacgccgct ggtagccata
     1021 tccagacgat gcaccgattc tgcctgcgga taatctcgct gaatgcgcgt catcacgctg
     1081 tctttgtgct cttccagacg acccggcaca gacaacaaac cgctcggctt gttgaccacc
     1141 ataatatggt catcctgata caggataacc aaccagggtt cctgcggtgg attgtagttt
     1201 tccatcccca ttttcggctc cgttactgat gcgttacaac gatcaaacgc agggcatcca
     1261 gacgccaacc tgcctgatcc aggctttcca ttacctgctg acggttgctc tcaatggcgg
     1321 tcagttcgtc gtcacgaatg ttcgggttca ctgcacgcag agcttccaga cgagacagct
     1381 cggcagacag tttttcgtcg gcttcgttac gtgctgcatc aatcaatgca cgggcagatt
     1441 tctcgatctg cgcttcaccc agttgaagga tagcgtgaac atcctgctgc acggcgttaa
     1501 ccagtttgct gccggtgtga cggttaaccg cgttaagctg gcggttaaag gtttcaaact
     1561 ctacctgcgc cgccaggttg ttgccgtttt tatccagcag catacgtacc ggcgtcggtg
     1621 gcaggaagcg gttgagctgc aactgcttcg gagcctgggc ttcaaccaca taaatcagtt
     1681 ccaccaacag cgtacctacc ggcaacgctt tgttttttaa cagtgaaatc gtgctgctac
     1741 cggtatcgcc agaaagaatc agatccagac cgttgcggat cagcggatgt tcccaggtaa
     1801 taaactgcgc atcttcacgc gccagcgcca cttcacgatc aaaggtgatg gtgatgccat
     1861 cttccgacag gccagggaag tccggcacca gcatatgatc ggacggcgtc agcacgatca
     1921 tgttgtcgcc gcgatcgtcc tgattgatac cgataatatc gaacaggttc atggcgaagg
     1981 cgatcaggtt ggtatcgtca tcctgctctt caatgctttc tgccagtgcc tgggcttttt
     2041 cgccaccgtt ggagtggatt tccagcaggc ggtcacgacc ctgttccagc tgtgctttca
     2101 gcgcttcatg ttgctcgcgg cagtttttga tcagatcgtc aaagccttcg gtttgatccg
     2161 gtgaagccag atagttaatc agatcgttgt atacgctatc gtaaatagtg cgtccggtcg
     2221 ggcaggtgtg ctcaaatgca tccagacctt cgtgatacca gcgcaccagc accgactgag
     2281 cggttttctc cagataaggc acatggatct gaatatcgtg cgcttggccg atacgatcca
     2341 gacgaccaat acgctgctcc agtagatccg ggttgaatgg caggtcaaac atcaccatgt
     2401 ggctggcgaa ctggaagtta cgtccttcag aaccgatttc tgagcacagc agtacctgtg
     2461 cgccggtgtc ttcttcggca aaccaggcgg cagcgcggtc acgttcgata atcgacatac
     2521 cttcgtggaa caccgcagcg cgaatacctt cacgttcgcg cagtacttgc tccagttgca
     2581 gcgcagtggc agctttagcg cagatcacca gcactttctg agagcgatgg ctggtcaggt
     2641 agcccatcag ccactcaacg cgcggatcga agttccacca ggtggcgtta tcaccttcaa
     2701 attcctgata aatacgctcc gggtagagca tatcgcgggc gcggtcttcc gcacttttac
     2761 gtgcgcccat aatgccggag actttaatag ccgtctgata ctgcgtcggt agcggcagct
     2821 taatggtgtg cagctcgcgt ttcgggaagc ctttcacacc gttacgggtg ttacggaaca
     2881 gcacgcggct ggtgccgtgg cgatccatca gcatcgaaac cagctcctga cgggcgctct
     2941 gggcatcttc gctgtcgctg tttgctgctt gcaacagcgg ctcgatatcc tgctcgccga
     3001 tcatctcgcc aagcatgttc agttcgtcat tgctcagttt gttacctgcc agcagcatgg
     3061 caacggcgtc cgctactgga cgataatttt tctgctcttc aacgaattgc gcgaaatcgt
     3121 ggaaacggtt cgggtccagc agacgcagac gggcgaagtg gctttccatt cccagctgtt
     3181 ccggggttgc ggtcagcagc agaacgcccg gcacgtgctc tgccagttgt tcaatagcct
     3241 gatattcgcg gctcggcgcg tcttcgctcc acaccaggtg atgcgcttca tcgaccacca
     3301 gcaggtccca ctcggcttca cagagatgtt ccagacgctg tttgctacgg cgggcaaaat
     3361 ccagcgagca aatcaccagc tgttcggtgt cgaacgggtt gtaagcatcg tgctgagctt
     3421 cggcataacg ctcatcatca aacagcgcaa agcgcaggtt gaaacggcgc agcatttcta
     3481 ccagccactg atgctgtaag gtttccggga cgataatcag cacacgttca gcagcgccag
     3541 agagcagttg ctgatgaagg atcatcccgg cttcaatggt tttccctaaa cccacttcgt
     3601 cagccagcag gacgcgcggc gcgtggcggc ggccaacatc atgagcgatg ttgagctggt
     3661 gcgggatcag gctggtacgc tgaccgcgca ggccgctgta cggcatacgg aactgttcgc
     3721 tggaatactt acgcgcgcga taacgcagcg caaagcggtc catacggtca atctgcccgg
     3781 caaacaggcg gtcctgcggt ttgctgaaca ccagtttgct atcaaggaaa acttcacgca
     3841 gggctacgcc ggactcttca gtatccaggc gagtaccgat ataggtcagc aagccatttt
     3901 cttcttttac ttcttcgact tgcatctgcc agccgtcatg gctggtaatg gtatcaccag
     3961 ggttgaacat cacgcgggtc acgggggaat cactgcgtgc gtacagacgg ttttcaccag
     4021 tagatgggaa aagtaaagtg acagttcgcg catccaccgc gacaacggtt ccaagtccca
     4081 attcgctttc tgtatcgctg atccagcgtt gaccaagtgt aaaaggcata tgtgttcggc
     4141 tctatatctt taattgcagg caataaccac ccgctaccgt gcttatgagg tagtggtgtt
     4201 attcaggtcc aggaatggaa agggcgctat ggtactggat ggcaaagcat tcgtcacgca
     4261 tcaaaatggt atctggcgaa ctcttttttt gctcaaaata gcccaagttg cccggtcata
     4321 agtgtagcaa aattatcctc aataaaaggg agtattccct ccgccacggg ttgtagctgg
     4381 cgggtcagat agtgttcgta atccagtggt gaacgttggt agtccagcgg ctccgggccg
     4441 ttggtggtcc atacgtactt aatggtgccg cggttctgat attgcaaggg gcgaccgcgc
     4501 ttttggtttt cttcatcggc aagacgagcg gcgcgtacat gaggcggaac attacgctga
     4561 tattcgctca gcggacggcg aaggcgttta cggtaaacca gccgcgcatc cagttcaccc
     4621 gccatcagtt tgtcgatggt ttcgcggaag tactcctgat atggctcgtt gcggaagata
     4681 cgcaggtaaa gctcctgctg aaactgctgg gccagcggcg tccagtccgt acgcacggtt
     4741 tccagccctt taaacaccat ccgctgtttg tcgccctcct gaatcagccc ggcataacgc
     4801 tttttactgc cggtatcggc tccgcgaatg gttggcatca gaaaacggca gaaatgggtt
     4861 tcatactcca gttccagtgc gctggtcagc tgttgttttt gcagcgtttc tgcccaccag
     4921 acgttaacgt gctgcaccag tgcacgaccg attttcgtcg cttcttcttc cgaatgtgcg
     4981 cctttcagcc agacaaacgt tgagtcggta tcgccgtaga tcacgtcgta gccctgcgct
     5041 tcaatcaacg ctttggtttg ccgcatgatc tgatgaccac gcatggtgat cgacgatacc
     5101 agccgcggat cgaagaagcg gcaggcggtg gtgccgagca cgccataaaa ggcattcatg
     5161 atgattttca gcgcctgcga cagcggtttg ttaccctggc gtttggcttc atcgcgtccg
     5221 tgccagatgt tagtcacaat ctccggcagg caatgttttt ctcgcgagaa ccaggcatcg
     5281 agaaagcctt cggtgctgtg ctctggatca ggctgcgcca tgccttccac cagcccgacg
     5341 ggatcaatca gaaaggtgcg gatgatcgac gggtacaggc ttttgtagtc cagcaccagc
     5401 accgaatcat aaagcccagg tcgcgaatcc atcacgtagc cgccagggct ggcgtgcggc
     5461 ggcacttcgc cgagattagg cgcgacataa ccagcgcgat gcatacgggg aaaatagaga
     5521 tgaccaaacg ctgccaccga cccgccgtgt cgatccaccg gcaggccgtt caccgttgct
     5581 cgttcgagca aaaacggcat gatttcagtt ttatggaaga tctgcgtcac cagctcgcaa
     5641 tctttcaggt tataagttgc cagcgcaggt ttatcttcgg cgaaacggcg gtcaatttcg
     5701 tccattcgat cccacgggtt atcgatagat tttccttcgc ctaatagctc ctgagcgaca
     5761 gtttccagcg agaatgaaga gaaattccag aacgcggatt tcagcgcctc gataccgtcg
     5821 ataattagcc gacctttagc ctgggcaaaa aagacgccgt ttttaaagcc gtgctcgcgc
     5881 cactccagct cactgttatc gcgccccagc cgcagcggaa tacgataacg ctcggcatgt
     5941 ttttgcagca ttcgcagatc aaactgcacc acgttccaac cgatgatcac atcaggatcg
     6001 tagtttgcaa accaggcgtt gagtttttcc agtagctgcg ggcggctggc gacgtactcc
     6061 agttcaaaat cgagcgcgga ggcgtcgcca ttctctggtc ccagcatata aacgatgcgc
     6121 tgcccgcagc cctccaggcc gatgcagtac agctctccgt ggcgggtggt ttcaatatca
     6181 atagaaaccc acttgagcgg cggacgatag tcgggatgcg gtttcagacg ggcattaacg
     6241 atagcgccat tgtgcatatc accctcgacc cacaccggtg aggtgataaa ccgctccatc
     6301 agatagcgtt ctggcgggcg cacatcggcc tcgtagacgg taacgccacc ttcacgcagg
     6361 cgcttttcgt aattcatcaa ctggcgatgg gcgcgacagt aaaggccata caccggctgg
     6421 cggtgaaaat cctttaacgc cagcggcgtc aggcgaaagc cttgttcacc ccgcaaaata
     6481 tgctgagcgc ggggaacctg atcggcggga ataaacgcca cggactcttg cggtgcaagc
     6541 gtaacctgca acggcccgtt gtccgtcgcc agccagaagg agacctctgt tccttgcggg
     6601 gtatcccgcc agtgtcgggt taagataaaa cctgcctgcg ccacgctgaa aatccatcaa
     6661 aaaaccaggc ttgattatag cctggtttca tttgattggc tgtggtttta tacagtcatt
     6721 actgcccgta atatgccttc gcgccatgct tacgcagata gtgtttatcc agcagcgttt
     6781 gctgcatatc cggtaactgc ggcgctaact gacggcagaa tatccccata taagcgacct
     6841 cttccagcac gatggcgtta tgcaccgcat cttcggcatt tttgccccat gcaaatgggc
     6901 cgtgagaatg gaccagaacg ccgggcattt gcgctgcatc gataccctgt ttttcgaagg
     6961 tttctacgat gacgttaccg gtttcccact catattcgcc gttgatttct gcgtcggtca
     7021 ttttgcgggt gcagggaatg gtgccgtaga aatagtcggc gtgggtggtg ccggttgctg
     7081 gaatcgactg acccgcctgc gcccagatgg tggcgtggcg cgagtgcgtg tgcacaatgc
     7141 cgccaatgga cgggaatgcc tgatagagta gtcggtgagt tggcgtatcg gaggagggct
     7201 ttttcgcacc ttcaaccact tcaccggttt cgatgctgac cacgaccata tcgtcagcgg
     7261 tcatgatgct gtaatcgacg ccggaaggtt tgatcacaaa gacgccgcgc tcgcgatcaa
     7321 cggcgctgac gttgccccat gtgagcgtga ccaggttgtg ttttggcagc gccaggttgg
     7381 cttctaatac ctgacgtttg agatcttcta acatgttgac tccttcgtgc cggatgcgcg
     7441 ttgcttatcc ggcctacaaa atcgcagcgt gtaggccaga taagacgcgt cagcgtcgca
     7501 tcaggcgctg aatgccggat gcgcgttgct tatccggcct acaaaattgc agcgtgtagg
     7561 cctgataaga cgcgccagcg tcgcatcagg cgttacatac cggatgcggc tacttagcgg
     7621 cgaaacccgt aatacacttc gttccagcgc agcgcgtctt taaacgctgg caggcgggtg
     7681 tcgttatcaa tcaccgtgat ttcaatgtcg tgcatctcgg cgaactggcg catatcgttg
     7741 aggttcagcg catggctgaa gacggtatgg tgcgcgccac cagcgaggat ccacgcttcg
     7801 gaagcagttg gcagatccgg ttgcgctttc cacagcgcat tcgccaccgg cagtttcggc
     7861 agggagtgcg gtgttttcac cgtgtcgatg cagttaacca gtagacggta acgatcgccg
     7921 agatcaatca agctggcgac aatcgctgga ccggtttggg tattgaagat caggcgggca
     7981 ggatcgtcct taccaccaat accgagatgc tgaacgtcga ggatcggttt ctcttctgcg
     8041 gcgatcgacg ggcagacttc cagcatatgg gagccgagca ccaggtcatt gcctttctcg
     8101 aagtgatagg tgtagtcctc cataaaggag gtgccgccct gcagaccggt tgacatcacc
     8161 ttcatgatgc gaagcagggc ggcagttttc cagtcgcctt cgcccgcaaa gccgtaaccc
     8221 tgctgcatca gacgctgtac ggccagacca ggaagctgtt tcagaccgtg caaatcttca
     8281 aaggtggtgg tgaacgcgtg gaagccacct tgttccagga aacgtttcat ccccagctca
     8341 atacgcgccg cttccagcac gttctgtcgt ttttcgccgt ggatttgtgt ggcaggcgtc
     8401 atggtgtagc agctttcgta ctcatcgacc agcgcgttaa catcgccgtc gctgatggag
     8461 ttcaccacct gcaccagatc gccaaccgcc caggtattga cggagaaacc gaacttgatc
     8521 tgtgcggcaa ctttatcgcc atcggtgacc gccacttcac gcatgttatc gccaaatcgg
     8581 cagactttca gatgacgggt atcctgttta gataccgcct gacgcatcca ggagccgata
     8641 cgctcatggg cttgtttatc ctgccagtga ccggtaacca cggcatgttg ctgacgcata
     8701 cgcgcgccaa tgaagccgaa ctcgcgaccg ccgtgcgcgg tctggttcag gttcataaag
     8761 tccatatcga tgctgtccca cggcagcgcc gcgttgaact gggtgtggaa ttgcagcaac
     8821 ggtttgttga gcatggtcag gccgttgatc cacattttgg ccggggagaa ggtgtgtagc
     8881 cacaccacca gaccagcgca acgatcgtcg taattagcat cacggcaaat agcggtgatt
     8941 tcatccggcg tggtgcccag cggtttcagt accagtttgc agggcagttt cgcttccgta
     9001 ttcagcgctt tcacaacgtg ctcggcatgt tgggtgacct gacgcagggt ttccgggcca
     9061 tacagatgct ggctgccaat tacaaaccac acttcataat tatcaaaaat cgtcattatc
     9121 gtgtccttat agagtcggaa cggcctgggc agcctgtgcc ggggcggaag ttggaagata
     9181 gtgttgttcg gcgctcatcg cccattgctg atagcggcga taaagctgtt caaagcgttg
     9241 tgcctgttcg ctgcgcggtt gcagggtttt ctctaccgca ctggccattt tttgctgggc
     9301 tgatgggatg tctgcgtgca ctttcgcggc gacggcagca aaaatcgccg caccgagcgc
     9361 acagcattgg tcagaggcaa caatttgcag cgggcgattc agcacgtcgc agcaggcctg
     9421 cataatgacc tggtttttcc gcgcgatgcc gcccagcgcc atcacgttat tgacggcgat
     9481 cccctgatcg gtaaagcact ccatgattgc gcgtgcgcca aaggcggtgg cagcaatcaa
     9541 accgccgaac agcagcggag cgtcggtagc gaggttaaga tcggtaatca cccctttcag
     9601 gcgttggtta gcgttcggtg tgcggcggcc gttaaaccag tcgagcacca ccggcaggtg
     9661 atccagagac ggatttttgg cccatgcttc ggtcagcgcc ggaagcagtt gtttctggct
     9721 ggcgtcgatt tgctctttta gttccggatg ctgggcggca agctgttcca gcggccagcc
     9781 gagtacgcga ccgaaccagg cgtagatatc accaaacgcc gattggcctg cttccagacc
     9841 gataaatcca ggcaccacgc tgccatcaac ctgaccgcaa atacctttca ctgcccgctc
     9901 gccaacgctc tgtttgtcgg caatcagaat gtcgcaggtg gaagtaccga taacttttac
     9961 cagtgcgtta ggctgtgcgc ctgcaccaac tgcgcccata tggcagtcaa acgcgccgcc
    10021 ggaaatcacc acgctttcag gcaggccgag acgctgcgcc cattccgggc ataaggtgcc
    10081 caccggaata tcggcagtcc aggtatcagt gaacagcggg gaaggcaaat ggcgattgag
    10141 gatcggatcc agctcatcaa agaaactggc tggcggcagg ccgccccagc tttcgtgcca
    10201 tagcgactta tgcccggcgc tgcaacgtcc gcgacgaata tcctgcgggc gcgtggtacc
    10261 ggaaagcaga gctggcaccc agtcgcacag ctcaatccac gatgcggcag attgcgccac
    10321 ggcgctgtcc tggcgagtca catgcaggat ttttgcccag aaccattcgc tggaataaat
    10381 accgccaata tagcgggagt agtcaacatt gcccggcgcg tggcacaaac gggtaatctc
    10441 ttccgcttct tcaaccgcag tgtggtcttt ccacaatacg aacatcgcgt tcgggttttc
    10501 ggcaaactcc gagcgcagcg ccagcacgtt accgtcggca tcaatcggtg cgggcgtcga
    10561 gccggtactg tcaacgccaa tcccgaccac agctgcgcgc tgttcgacgc taagttctgc
    10621 aagcacggtt ttcagtgccg cttccattga ctcaatgtag tcacgcggat gatgacggaa
    10681 ctggttattc ggggcatcac aaaattgccc tttttgccaa cggggatacc actctacgct
    10741 ggtggcgatt tcttcaccgg tggtgcagtc caccgccaaa gctcgcacag aatcactgcc
    10801 aaaatcgagg ccaattgcaa tcgccatcgt ttcactccat ccaaaaaaac gggtatggag
    10861 aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt atggataaaa
    10921 atgctatggc atagcaaagt gtgacgccgt gcaaattatc aatgtggact tttctgccgt
    10981 gattatagac acttttgtta cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa
    11041 tgcttttaat aagcggggtt accggttggg ttagcgagaa gagccagtaa aagacgcagt
    11101 gacggcaata tctgatgcaa tatggacaat tggtttcttc tctgaatggc gggagtatga
    11161 aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aacgcccatc
    11221 tggtggcggg tttaacgccg attgaggcca atggttatct cgattttttt atcgaccgac
    11281 cgctgggaat gaaaggttat attctcaatc tcaccattcg cggtcagggg gtggtgaaaa
    11341 atcagggacg agaatttgtc tgccgaccgg gtgatatttt gctgttcccg ccaggagaga
    11401 ttcatcacta cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg gtttactttc
    11461 gtccgcgcgc ctactggcat gaatggctta actggccgtc aatatttgcc aatacgggtt
    11521 tctttcgccc ggatgaagcg caccagccgc atttcagcga cctgtttggg caaatcatta
    11581 acgccgggca aggggaaggg cgctattcgg agctgctggc gataaatctg cttgagcaat
    11641 tgttactgcg gcgcatggaa gcgattaacg agtcgctcca tccaccgatg gataatcggg
    11701 tacgcgaggc ttgtcagtac atcagcgatc acctggcaga cagcaatttt gatatcgcca
    11761 gcgtcgcaca gcatgtttgc ctgtcgccgt cgcgtctgtc acatcttttc cgccagcagt
    11821 tagggattag cgtcttaagc tggcgcgagg accaacgcat cagccaggcg aagctgcttt
    11881 tgagcactac ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac
    11941 tctatttctc gcgagtattt aaaaaatgca ccggggccag cccgagcgag ttccgtgccg
    12001 gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc ataattggta acgaatcaga
    12061 caattgacgg cttgacggag tagcataggg tttgcagaat ccctgcttcg tccatttgac
    12121 aggcacatta tgcaagcatt gctggaacac tttattaccc aatccaccgt gtattcattg
    12181 atggcggtgg tgttggtggc ctttctggag tcgctggcgc tggtcggttt gattctaccc
    12241 ggtacggtgc tgatggcggg gctgggagcg ctgattggca gcggcgagtt aagtttctgg
    12301 cacgcctggc tggcagggat cgtcggttgt ctgctgggcg actggatctc cttctggctg
    12361 ggctggcgtt ttaaaaagcc attgcatcgt tggtcatttc tgaagaagaa caaagcatta
    12421 ctcgataaaa ctgaacatgc gttgcatcaa cacagcatgt tcaccattct ggtcggtcgt
    12481 tttgttggcc cgacgcgtcc gctggtgcca atggtggcgg gaatgctgga tctcccggtg
    12541 gcaaaattta tcacgccgaa tattatcggc tgcctgctgt ggccaccgtt ttacttcctg
    12601 ccggggattc tggcgggtgc ggcgatcgat attccagccg gaatgcagag cggtgagttt
    12661 aaatggttgc tgctggcgac ggcggtattt ttgtgggttg gtggctggct gtgctggcgg
    12721 ttatggcgca gcggcaaagc aaccgatcgt ttgagtcatt atttgtcccg cggtcgtttg
    12781 ttgtggctga cgccgttgat ttctgccatc ggcgtggtgg cgctggtggt gttagttcgc
    12841 catccgttaa tgccggtgta tatcgatatt ttgcgtaaag tggttggagt ttagggcata
    12901 gtcttgtgtg gggcgcttga tgcggacaaa acgcacccgt aggtcggata aggcgttcac
    12961 gccgcatccg acagtgcata ttaacctgta attcccaaca gcgccgaagc actcgcctta
    13021 ccgctcaaca actcattggt cataccctgc caggcgattc gcccgtcggc gactactacc
    13081 gagcgcgtgg caatccgcgc cgcatcttcc acactgtgcg acaccatcaa tagcgtcatt
    13141 ttttgctgct ggcagctagt gctcaccagc gtcaacatct cctgacgtag cgccggatcg
    13201 agcgcagaga acggttcatc gagcaataaa atcggctgtt cgcgtaccag acaacgcgct
    13261 aacgccacgc gctgtcgctg accgccggaa agctcgcccg gtaaccgcgc cattaaatta
    13321 tcaatcccca tctgacgggc aatcgcgtgc attttctcct gctgtaccgc gttcagcttc
    13381 aatcctggat taagccccag cccgatgttc tgcgcgaccg tcaggtggct gaacaggttg
    13441 ttctcctgaa acagcatcga caccggacgg cgtgacggcg gcgtcgtggt gtgatcaacg
    13501 ccgtcgatag tcagcgcacc gctggctggt gtcagaaaac cggcgatcaa gttcagcagg
    13561 gtacttttac ccgcgccgct tggcccgagg atcgccacct gctcgccgcg ttctaccgtt
    13621 aagctaaaac gcatcggcaa atggtgataa agccaggtga tatcagtcag ttttaacatt
    13681 tcgccccggt attttttcaa tcacgctaaa cagcagaaaa cagagcagca gcagaattaa
    13741 cgcggtgacc gcgccgtcct ggctgcgata ggagccaatt tgctggtaga gataaaacgg
    13801 cagggtgcgg aaatcatcgt taccgaacaa cgccaccacg ccaaaatcac caatcgacag
    13861 cacgcaggca aaggccagcg cctgcgccag cgggcgtttc agggcgcgca gctccactac
    13921 ttttaagcgt gaccagcctt caatccccag cgactgacat aacatgctgt agcgggcggt
    13981 gatatcgcgc atcgggtttt ccagcacttt cagggcgtag gggatcgcca ttaacgcatt
    14041 ggtgaaaatc acaatgccgt cagcagattg tggcaggccg atagtgttgt tgagcagtaa
    14101 aaagaagccc gttgccagca caatccccgg catagcgagg atcaacatgc cgctcatctc
    14161 cagcgcctgg cccgccagca ttttttgccg cgcccgcagt tcgcgactgc tccatagcag
    14221 catcatggtc agcactacgc acaatacacc tgccgccagc gcaatacgca acgaggtcca
    14281 cagcgcctgc cacagcaccg gttgtgccag cacttccggc aactggcgat ttaccccatc
    14341 gacgatcacc gccagcaacg gtggcagcaa cagcagcagc gccagcacaa ttaacatcgt
    14401 gtcgcaaatg cggctatgca gacgatcgtc cgggtcgcgc cagccttgca gcagcgtggt
    14461 gccgggcgca atggccttac tcagtcgctg actcaacagc accagcccaa ggcaacacac
    14521 catctggatc agcgccagca tcgccgcgcg ggcaggatcg tagtcgtaac tcagcgcctg
    14581 atagattgcc agctcgatag tggttgcctg cggaccgccg cccagcgata acacggtggc
    14641 gaagctggcg aaacagagca taaagataag cgcggtaacc ggcgggattt gtcgccgtaa
    14701 ccacggccat tcgacgaagc ggaaaaaatg ccagccgcgc atcccaagct gggcggcaag
    14761 ctgacgttgt tcgccgggaa tgttttccag tgcctggagt aataagcggc tcgccatcgg
    14821 cagattaaaa aacacatggg ccagcaaaat gccttgcagg ccgtagggcg aaaaggtcca
    14881 ctccagaccg agcgattggc agagcgatgc cagccagccc tggcgaccat agacgctaag
    14941 aatgccgaaa acagcaacca gcaccgggag gatcaaggtc attgcgcaca gacgcaacag
    15001 cgccagccga cccggaaagc gcctgcgata gagcgcgcgg gcgaggaata tcgcgggtac
    15061 gacagagagt agcgccgaga gaaacgcctg ccagaaggag aagcgcacca catgccacag
    15121 gtagctgtcc tgccagactg ccgaccagtc accctgcggc gcgttccacc acagggcgag
    15181 aaacgccgcc agcgcaaccg ctaccaccag cgtggcggcg cttacacctg gaattaacca
    15241 gccgggaatt aacggctgac ggcgcgttgc cattcgctaa tccatgcctg acgttgtgcc
    15301 gccacttcgg ctggcgtgaa ctccagcgtg gttgcgggtt tggtcaattt ttcaaaaccg
    15361 gcaggcagcg tgacgtttgc caccggatac atccagttgc cggttgggat cgcattctgg
    15421 aaagccggag aaaccataaa ctggaggaat ttttgcgcca gctccggctg cttgctggca
    15481 gcggtgcgag cggcgacttc cacttgcaga tagtgacctt cgctgaagtt cgcggcggcg
    15541 tagttatctt tcttctcttc gagaatgtga taagccggag aggtggtgta actcagtacc
    15601 agatcgcttt caccttttaa aaacaggccg taggcttcgc tccagccttt ggtgaccgtg
    15661 accgttttct tcgccagttt ctgccaggct tgtggggcgt catcgccata gactttttgc
    15721 atccatagca acagacccag ccccggtgta ctggtgcgcg gatcctgata aatcacccgc
    15781 cagttttgat cgctctcaac cagttctttc aggctttgtg gcgggttttt cagtttgttc
    15841 ttgtcataaa cgaaggcgaa gtagccataa tcaaacggta cgaaaatgtc attattccag
    15901 ccgccgggaa cgttaacggc atccgctgcc acaccgcttt tggcaaacag tccggtttta
    15961 ctggcggcgt ctaacagatt gttatccagc cccagcacca catcggcttt actgtttttg
    16021 ccttccatcc gtagacggtt gagaagcgaa acgccatctt ccagcgccac cagtttcagt
    16081 tcgcaattac agtcggcttc aaaggctttt ttaaccaccg gaccaggccc ccagtcggcg
    16141 gcgaaggaat cgtaggtata aacagtcaga acgggtttag cgaaaacggg cgctgtgcac
    16201 agcaacagca ggggaagaca tttttttaac actttgcacc tcaaaaaaga gtggcaaagg
    16261 acttgagaag gagcctcaaa tcccttcgcc ggcgttatcc ggatcaggtt cgacgggtat
    16321 tttctcagcg cacgcgtccg cgtggcaccc cgttgagaac ggcgttagtg tagtgatttt
    16381 gttatcaacc agcaatccat agagaaggcg tattaaaaaa gccaatattc gttcagctat
    16441 caagagcgta tttgcatagc tggagagcgt tgatccgaaa gaatcccttc gctacagtca
    16501 aaataggtaa cgtaaccgtt tattgcgtga atgagcgatt tttgacagta ttcttattca
    16561 ggacgcttat tctgagtttt ttattcagag tggagctatc agcatgaaag taagaaaccc
    16621 agaacagatt agtatccctg ctagtaatac cactaaagat ccaggcctta ctaattctca
    16681 gattatcagg atggctaact tggtcaaaaa gaccgagaat atgaatatct ttgaagagct
    16741 atgggagacg ttacgcaatc tcttccagtc agataaacat tcccagactg cggcaaggca
    16801 aattctgaag gatgcatttt atttccagaa tagtgatgac tactcaaaat actttaccgg
    16861 ggctgtcgac gggaaggcgc gtgataagtt aactcactgg ctgataaaat ttaatgaact
    16921 caaagaatac gcaaaagatc cggaaaatat ggccgcgaag gcctcactct cgccagaagg
    16981 tacgttatgc gtcagttttt ttattggcga tgaggcgata tttactctgg agttgcagct
    17041 gaaaaagagc accaggaccg gggggattga tctgagtaat gcttacttta acggggttgt
    17101 catttgtggt attgactgtc tcgaggttga cctgagcaat gctgaaacga acaatagccg
    17161 ttggtatgac tgacctgcgc gaggttaacc tgaaaaagct gacctttccg gccagaacaa
    17221 ggctagactg cgccgttatt gattaactca gaatatgaaa gaggggctgg aatgcccctt
    17281 ctttaatctt atttgcgacg ctaatcagac gatgcataaa acagagaaaa tataaaagta
    17341 atgtatttta tcattccgtc tgaatataaa aaatgcgctc atgaccgcca aaaggcgcaa
    17401 gctactctgg atccggcggc gcaaaccacg ctgatttaaa atcaaaccag ccgagggtgt
    17461 tcatgcgcag gccgcgcata ctgcgttgtc cctgaatgat cagccagtgg tggataagtg
    17521 gcaccattgc tttgctggcg accagttgtt ggcaccagtt cgccaggttc atttcgccat
    17581 tgcgccagcg ggcggcgtcg acttgccagt cgattggaat acagtgttgg agcagcggta
    17641 cctcgcacag gtgcgcgaac agcgaaaaat ccagcggcag cgtaaagttg gcgctgttaa
    17701 gccagatatc gctctcgatc tctccttcat gccactgatc gtagctgatc tctttgattt
    17761 ccagtgtgac ctggtggctt gccagaatct gctgcatgat cccggcaatc acccgatgct
    17821 cactgtgatc ctgataaaag gtcagggtga ggctttccag accagccggt ttttcgctcg
    17881 ttatggtgcg ggcatgatgc caacggggga gcagtccata agccgggaac cacagttgct
    17941 ggtactgttc ctcagcgaaa tagaccagat tagttggaga aagcacatag cttacccagt
    18001 ccctgacttg ctgattcgcc ccgcgatggg tgcggctatc gaacagtaaa tagtagcaac
    18061 cttcctccag gcggctttca atctcttttt cctcgccctg tggacctttt agcatcagcc
    18121 ctccggctgg ctcgtcggca atttccggca gaacccagac gttaacttca tcgattaatg
    18181 cccggtaacc gaagaagtca tcgaatgcct gaattttcag ttgattggtg ctgttgcgaa
    18241 tcaccgcata cggaccggtg ccgatgggat ggctggcaaa gttactgagg gtttcccatt
    18301 cgcgcggcag gatcatcgcc ggaacttgtc ccagcagtaa cggcaaccag cgatccggct
    18361 gcgtgaggtg gatatccagc gtccagggcg tcggcgacac aatgtcagca atatgcgaat
    18421 agagcggcag cgtattgatt cgttttaaag aggcgatcac gtcgtccatt tccagttcac
    18481 gaccatgatg aaaatggact cctggacgca aaaagaaacg ccagtgaagc ggtgatattt
    18541 gctgccagtg gtgggcgatg tctgcttcca gttccccatt ttcctcattt atgcgcgtta
    18601 gcgaactgaa gatttgccgg gcgatatggg tttcggaacg gcgcaatgcg ctgccaggta
    18661 gcagattacg caacggacga tagtagagca cgcgcaggat gtgccgcccc tggcggaagc
    18721 tgcggcccag atgagaaacc agcatttgcc tcacagtcgc tttgtcgcca actaactgca
    18781 ctagttggtc gatacggtcc tgctccagca ggtcttccgc ccgctgttgc tgaagcgcca
    18841 gcccggtata gaggaatgtc agacgcgagc gtttaccacg cccgacttcc gcttcccacg
    18901 tcagccagcc gcgatcctgc atggtgttga gcagggtacg catatgacga cgcgagcagc
    18961 tcaataacgc tgccagttcg ttgagcgttg tgtcctgcga tttaccctcg cagcattgcc
    19021 acaggcggat gaactgttgt tgcagacgag cagatggcat aaaaggggaa ctcctgcgca
    19081 aaagacagca attttatttt ccctatatta agtcaataat tcctaacgat gaagcaagga
    19141 ggtgccccat tcgtcagttt tatcagcact attttaccgc gacagcgaag ttgtgctggt
    19201 tgcgttggtt aagcgtccca caacgattaa ccatgcttga agaactgatg cagtgggagg
    19261 ggagtcattc tgactactga tttgcggaca tcatgtgtga ctgagtattg gtgttaatcg
    19321 ccacgccagc agtgattatc tgctggcttt tttctttttg gcgattgagt atcggacggt
    19381 gtacaaacta acgcataaac gcgggttgct tcgcttcata agccgcaatg gcgtcgtcgt
    19441 gctgcaaggt aagcccaatg ctgtccagac cgttcatcat gcagtggcgg cggaaggcat
    19501 caatggtaaa gcgataggtt ttctcacccg ctttcacctc ttgcgcttcc aggtctacat
    19561 caaaatgaat ccccggattg gctttcacca gcgcaaacag ctcatccact tctgcgtcgc
    19621 ttaatttcac cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac
    19681 tcggcgcaat caccacctta aaaccgtagt cggtcaatgc ccagggcgcg tgctcacgcg
    19741 aggagccaca gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcgaga
    19801 agttcagcac gaagtccggg tttggctgtt ggcctttttc atccagaaaa cgccagtcgt
    19861 taaacagatg cgcgccaaaa ccagtacggg tcactttctg caaaaactgt ttcgggatga
    19921 ttgcatcggt atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa
    19981 atttctctgc catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atggccggtc
    20041 acagccgcgg cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctga
    20101 cggccttcaa agttacggtt gctggtggag gcacaacgtt cgcccggatt cagacggtcg
    20161 ttgttcatcg ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata
    20221 aagattttat ccagaccttc cgcttccgcc tgcgctttta ccggaccaga gccgggaacc
    20281 accagcgcct gtacgcctgg cgcgactttc cgccctttgg cgatttccgc cgccgcgcgt
    20341 aaatcttcaa tacgtgagtt ggtgcaggaa ccgataaaca ctttgtcgat agccacttcg
    20401 gtcagcagaa tacccggttt cagccccata taggccaacg ctttttctgc cgacgcgcgt
    20461 tcaaccggat cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg
    20521 cctgggttag ttccccaggt gacctgcggt gaaatctctt ctgcctgcaa agtgacaacg
    20581 gtatcgaaag ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg
    20641 aaatctttgc ctttcggcgc atgcagacgg cctttgacat agttaaaggt ggtttcgtca
    20701 ggtgcaacca gaccggcttt cgcgcccatt tcgattgcca tattgcacag ggtcatccga
    20761 ccttccatgc ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg
    20821 cctgcgctac cggttttacc gataattgcc aacacgatat cttttgcggt aatgcccggc
    20881 gcggctttgc cctggacttc aattttcatg gtcttcgcac ggccctgttt cagggtttgc
    20941 gttgccagta cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg
    21001 ccgtgggtgg cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtcacgccc
    21061 tgttccggcc ccattacgtg gacgatccct tgataggggt gattcaggtc atacagttcg
    21121 acgccaaatt ctttgcagtt tttgatcagc tcctgcatct ggatgcgcgc catttcaccg
    21181 caggcattaa tgtctttagt ctgggtagag acgttgtgat ccatggtggc gaaggttttg
    21241 cccggctgac gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc
    21301 acttcatgca ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac
    21361 actacgtgag cgtcgaataa tttttcgtat aacgtcttag ccatgattac accccttctg
    21421 ccacatagcg ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg
    21481 ctaaatcccc ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgg
    21541 aagccgcatc atcggcatcc aggctgtaac gcagcagcag tgccagcgac agaatttgcg
    21601 caatcgggtt ggcgatgttt ttacctgcga tatctggtgc cgagccgccc gccggttcat
    21661 acagtccaaa accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga
    21721 tcattgcgca ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa
    21781 actgtgatgg atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt
    21841 cgatatccgg gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt
    21901 gcagcacgtt ggctttatcg atcgacgtca ctttatggcg acgtttacga gcagattcaa
    21961 acgcgatgcg ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt
    22021 tttcatattg tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca
    22081 gttcgcgcac gcacaggatg tcgaagccgt ttgcggcaat gtctgcacgc agcggacaga
    22141 atgcttccag cccctgatac agttttgccg gacgcaggtt gctgaataat ttgaagtgct
    22201 tacgcaaagg caacagcgcg ccgcgttctg gttgctggtc cggtggtaaa tgttcccatt
    22261 tcgggccgcc taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg
    22321 caggcggcag tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg
    22381 tagtgatgcg catcgcaaag cggttgcgca cggcatccag cactttcagc gcctgggtca
    22441 tcacttccgg accaatgcca tcccccggca atacggcaat atggtaattc ttcgacatca
    22501 cacggtttcc ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc
    22561 tgtggcacgc cagatattgt taagtacgtg caccatggct ttggcggagg actcgacaat
    22621 atcggtggcc aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac
    22681 ctgacccagc gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac
    22741 gttatagtca gtgatgcggt taatcgcctg atagacggca tcgaccggac cgttaccgtt
    22801 ggcggcttct gctttgactt cttcgccgca ggccagtttg acggcggcgg tggcgatatc
    22861 gttagagcca gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg
    22921 cttaccgatg aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc
    22981 cagcttgagg aaggcgtcgt acaaattgtc taaattatat tcactttctt tataccccat
    23041 ctcatccatg cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat
    23101 ttggttcaga ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac
    23161 gccatcctgg tggataccgg aggagtgtgc gaatgcgccg ctgccaacaa tggctttgtt
    23221 tgccgggatc ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg
    23281 gtgattaatg gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac
    23341 ttcttccagg gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg
    23401 gcgtgcaccg gcatgtaccg ccgccagtga gttgccgacc gccaggccca aatcgtcgtg
    23461 ggtatgtacg gaaataatgg ctttgtcgat gttaggcacg cgttcataca ggccgctaat
    23521 gattccggcg aactcaaacg gcatggtgta gcccacggtg tccggaatgt tgatggtggt
    23581 ggcaccggca ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc
    23641 ggcatcttcg caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata
    23701 gatagcgcgt tcgatcacct cgtccagcgt gctgcgcagc ttggtggcga tgtgcattgg
    23761 cgaagtggca ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc
    23821 cacgtcgata tctttctcca cgcagcgagc taacgcgcat acacggctgt ttttaacctg
    23881 gcgagcgatg gtttgcactg attcaaaatc acccggcgaa gagacgggga acccgacttc
    23941 catcacgtca acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact
    24001 caagcttgcc tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg
    24061 ctggctcatg gtttgggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac
    24121 ccgcgcaatg gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc
    24181 actcgtctac cgcgcaaaga agatgcgttt agtagtagta gtagaccgat aaagcgaacg
    24241 atgtgagtca ttaaatcagc tccagatgaa tgcgatatgc ttttagagtt actggataca
    24301 aaaacggatg tcaaccctga cgcaataaaa acgtcccgcc agcatgagtt ctgcatccgt
    24361 aagattagct aattgtgctg ctgtggttaa aacaagcgat attaatttct gcttaactac
    24421 cgatgctttt catcggttga catatttcag aataaatttt tgcatctaat ccacgaggaa
    24481 aaaggggaca aaatgcacgc gtt
//
LOCUS       DAZKFZ010000057        24016 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000057 DAZKFZ010000000
VERSION     DAZKFZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            113..3865
                     /gene="yfaL"
                     /locus_tag="ACHOQW_002071"
     CDS             113..3865
                     /gene="yfaL"
                     /locus_tag="ACHOQW_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP9573153.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4006..4728)
                     /gene="ubiG"
                     /locus_tag="ACHOQW_002072"
     CDS             complement(4006..4728)
                     /gene="ubiG"
                     /locus_tag="ACHOQW_002072"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP9573154.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4875..7502
                     /gene="gyrA"
                     /locus_tag="ACHOQW_002073"
     CDS             4875..7502
                     /gene="gyrA"
                     /locus_tag="ACHOQW_002073"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP9573155.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7710..9338
                     /gene="yfaA"
                     /locus_tag="ACHOQW_002074"
     CDS             7710..9338
                     /gene="yfaA"
                     /locus_tag="ACHOQW_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP9573156.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWQPL"
     gene            9335..9958
                     /gene="yfaT"
                     /locus_tag="ACHOQW_002075"
     CDS             9335..9958
                     /gene="yfaT"
                     /locus_tag="ACHOQW_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP9573157.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9982..14496
                     /locus_tag="ACHOQW_002076"
     CDS             9982..14496
                     /locus_tag="ACHOQW_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP9573158.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEESKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAVNSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREAAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14497..16146
                     /gene="yfaQ"
                     /locus_tag="ACHOQW_002077"
                     /pseudo
     CDS             14497..16146
                     /gene="yfaQ"
                     /locus_tag="ACHOQW_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16151..16927
                     /gene="yfaP"
                     /locus_tag="ACHOQW_002078"
     CDS             16151..16927
                     /gene="yfaP"
                     /locus_tag="ACHOQW_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP9573159.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17202..20051
                     /gene="rcsC"
                     /locus_tag="ACHOQW_002079"
     CDS             17202..20051
                     /gene="rcsC"
                     /locus_tag="ACHOQW_002079"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP9573160.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20251..20901)
                     /gene="rcsB"
                     /locus_tag="ACHOQW_002080"
     CDS             complement(20251..20901)
                     /gene="rcsB"
                     /locus_tag="ACHOQW_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP9573161.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20918..23590)
                     /gene="rcsD"
                     /locus_tag="ACHOQW_002081"
     CDS             complement(20918..23590)
                     /gene="rcsD"
                     /locus_tag="ACHOQW_002081"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP9573162.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5874 a         5846 c         6539 g         5757 t
ORIGIN      
        1 taataacaaa atagctattg catcgggttg gacaatttac aaaagaaaga taacccatat
       61 tgtataatgc gtttaaatat gccgtcttat ataaaatgat ggtcagatta atatgcgggt
      121 tatctttcta cgcaaggagt atttatctct actcccgtca atgattgcat ctcttttctc
      181 tgctaacggt gtcgcggcgg ccattgattt atgccaggga tatgatatca aagcgagttg
      241 tcacgccagc aggcaaagcc tttcaggcat tacgcaggtc tggagtattg ccgatgggca
      301 atggctggtt ttttcggata tgaccaataa tgccagcggt ggggccgtat ttttgcaaca
      361 aggagcggaa tttacattat caccagaaaa tgaaactgga atgactctgt ttgccaataa
      421 caccgtttca ggagaatata ataacggcgg ggcaatattt gctaaagaaa actcaacgct
      481 gaatcttacg gatgttattt tttctggtaa cgtcgcaggc ggctatggtg gcgcaatcta
      541 ttcttctggt actaacgata ccggtgccat cgatttacgt gtcactaacg ccgtgtttcg
      601 caataacatc gctaatgacg gcaaaggtgg tgcaatttat accatcaata atgatatcta
      661 tttaagtgat gatgttttta acaataacca ggcatataca tcaacaagtt acagtgatgg
      721 cgatggcggc gcaatcgatg tcacagataa taatagcgac agcaagcatc cttcaggtta
      781 tacgataata aataacactg cctttacaaa taacactgcc gaaggttatg gcggggcgat
      841 atataccaat agcgcgacgg ctccctatct tattgatatt tctgttgatg acagctacag
      901 ccagaacgga ggcgtgttag tcgatgagaa caatagcgca gcaggctatg gagatggtcc
      961 ttcctctgcg gcgggtggct ttatgtatct cggcttaagt gaagttacct ttgatattgc
     1021 cgacggaaaa acgctggtta ttggcaatac agagaatgac ggagctgttg actctattgc
     1081 tggtaccggg ttaatcacca aaacaggttc cggcgatctg gtacttaatg cagataacaa
     1141 tgactttact ggtgagatgc agattgaaaa cggtgaagtt accctgggcc gcagcaactc
     1201 cctgatgaat gtcggcgata cgcattgcca ggacgatccg caagactgct acggtctgac
     1261 gatagggagt attgataagt accagaatca ggcagagcta aatgttggct ccacccaaca
     1321 aacctttgcg cactcattga cgggctttca gaatggcact ttaaatatcg atgctggtgg
     1381 caatgttact gttaatcaag gcagttttgc tggcaccatc gaaggtgctg gtcagctcac
     1441 cattgcgcaa aacggcagct atgtgctggc gggggcgcag tcgatggcgc taaccggcga
     1501 tatagtggtg gatgctggtg cggtgctttc gctggaaggc gacgcggcag atcttgccgc
     1561 tctccaggac gatccgcagt cgatcgtgtt aaacggcggt atgctcgatc tctctgattt
     1621 ctccacctgg cagagcggta catcatacaa agatggcctt gaagtcagtg gcagcagcgg
     1681 aacggttatc ggcagtcagg atgtggtaga tcttgcaggc ggaaacgata tgcatatcgg
     1741 cggcgacggg aaagatggcg tctacgtggt gatcgatgcg ggtgacgggc aggtcagcct
     1801 ggcaaatgac aatcaatacc tcggcacaac gcaaatcgct tccggtacgc tgatggtgag
     1861 cgacaactcg cagcttggat atacccatta taaccgccag gttatcttta ccgataagcc
     1921 acaagaaagc gtgatggaga ttactgccaa tgtcgatact cgctctacaa cgactgagca
     1981 tgggcgtgat attgaaatgc gcgccgacgg tgaagtggca gttgatgcgg gggtagacac
     2041 gcagtggggc gcactgatgg ctgacagcag cgggcagcat caggatgagg gtagcacatt
     2101 gactaaaacg ggggcgggta cactggagct gaccgccagc ggtacaacgc agtcagcggt
     2161 gagagtagaa gagggcacgc tgcaaggtga tgttgcggat atcttccctt atgcttcgtc
     2221 gctatgggtc ggtgacgggg caacgttcgt tactggcgcg gatcaggata ttcagtcaat
     2281 tgatgctact tccagcggca ctatcgacat cagcgatggt acggttttgc gcctgaccgg
     2341 gcaggatact tccgtcgccc ttaatgcctc actgtttaac tgcgatggga cgctggtgaa
     2401 tgccaccgat ggtgtgacgt tgacaggtga gcttaatacc aaccttgaaa ctgacagcct
     2461 gacttatctt tccaacgtga cggttaatgg caatctgacc aatacgtccg gtgcggttag
     2521 cctgcaaaat ggcgtcgctg gcgatacgct gacggtaaac ggtgattata ccggcggcgg
     2581 tacgctactg ctcgatagcg aattaaacgg cgatgactcg gtaagcgatc aattggtgat
     2641 gaacggtaat actgctggca acacaactgt ggtggttaac tccattacag ggattggtga
     2701 gccgacatcg acaggcatta aagtggttga tttcgcagct gatcccacgc agtttcaaaa
     2761 caatgcgcag ttcagtctgg caggcagcgg ctacgtcaat atgggagcgt atgactacac
     2821 gctggtggaa gataacaacg actggtatct gcgatcgcaa gaagtaacgc cgccatcgcc
     2881 acctgatcca gacccgactc ccgatcctga tcccacgcag gatcctgatc caacacccga
     2941 cccggaacct acgcctgctt accagccggt gttgaatgcc aaagttggcg gttatctcaa
     3001 taacctgcgg gcggcaaatc aggcgtttat gatggagcga cgcgatcacg caggtggcga
     3061 tggtcagacg ctgaatttac gtgttatcgg cggagattat cattacacag cagcggggca
     3121 actggctcaa catgaagaca cttctacggt gcaacttagc ggcgatctgt ttagcgggcg
     3181 ctggggcacg gatggcgagt ggatgcttgg gattgttggt ggctacagcg ataaccaggg
     3241 cgacagccgc tcgagtatga ccggaactcg cgccgataac cagaaccacg gttatgcggt
     3301 tgggctgacc tcaagctggt ttcagcacgg taagcagaag cagggggcct ggctggataa
     3361 ctggttgcag tacgcgtggt ttagcaatga tgtttctgaa catgaagatg gcgtggatca
     3421 ttaccattcg tcggggatta tcgcctcgct ggaagcgggg tatcagtggt taccggggcg
     3481 tggtgtggtg attgaaccgc aggcgcaggt gatttatcag ggcgtgcagc aggatgattt
     3541 taccgccgct aaccgtgcgc gcgtgtcaca atcgcagggt gatgatattc agacgcggct
     3601 gggtttacac agcgaatggc gtaccgctgt tcatgtcata ccaacattag atctgaatta
     3661 ttatcacgat ccccattcga cggaaattga agaagatgcc agcactatca gtgacgatgc
     3721 ggtgaagcaa cggggtgaaa taaaagtggg agtcacgggc aatatcagtc agcgagtttc
     3781 gctgcgtggt agcgtggcgt ggcagaaagg gagtgatgat tttgcccaga cggcagggtt
     3841 tttgtcgatg acggtgaaat ggtaatcgct gtctgaccat cactattgct ggatgcggcg
     3901 taaacgccta atcaggccta cggagagcat ttgtaggcct gataagacgc ggcaagcgtc
     3961 gcatcaggca ttggcgcggt tgccggatga atccaaacaa caacctcact tattctgcgt
     4021 gtgcagcata tagttcacat ccacgccggg gccgagttta aaagtattag tgatcgggtt
     4081 gtaatgtagc ccggttatat gtcgctcttt aaggcttgtc tgatctaccc agcccagcag
     4141 ttctgccggt ttaataaact tcttcacatc atgtgtacct ttaggcacca tgcgcaaaat
     4201 atattccgcg ccgactaccg ccatcagcca tgacttgccg ttgcggttaa gcgtcgagaa
     4261 aaagacatcg ccgcccggtt tcaccagttg cgcacaggct ctgaccactg actgcggatc
     4321 agggacgtgc tccagcatct ccatgcaggt caccacatca tactgcccgg catgttttgc
     4381 cgcgtgctct tccacggttt cctgcacgta atccacctga atgccgcttt ccagcgcgtg
     4441 cagctttgcc acctgcaatg gctcaaagcc catatccaga ccggtcaccg tcgcgccttc
     4501 gcgcgccata ctctcggcca gaatgccgcc gccacaaccg acatcgagca cctttttgcc
     4561 aaataagccg ccagcacgct cggcaatata gctcagacgc agcgggttaa tgcggtgcag
     4621 cggtttgaac tcaccttcca gatcccacca gcgggaggcg acggcttcaa atttagcgat
     4681 ctcttcgtgg tctacgttat ggtttaccgg cgatttttcg gcattcattg gcacttctac
     4741 tccgtaattg gcaagacaaa cgagtatatc aggcattgga tgtgaataaa gcgtataggt
     4801 ttacctcaat ctgcgcggct gtgttataat ttgcgacctt tgaatccggg atacagtaga
     4861 gggatagcgg ttagatgagc gaccttgcga gagaaattac accggtcaac attgaggaag
     4921 agctgaagag ctcctatctg gattatgcga tgtcggtcat tgttggccgt gcgctgccag
     4981 atgtccgaga tggcctgaag ccggtacacc gtcgcgtact ttacgccatg aacgtactag
     5041 gcaatgactg gaacaaagcc tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta
     5101 aataccatcc ccatggtgac tcggcagttt atgacacgat cgtccgtatg gcgcagccat
     5161 tctcgctgcg ttacatgctg gtagacggtc agggtaactt cggttccatc gacggcgact
     5221 ctgcggcggc aatgcgttat acggaaatcc gtctggcgaa aattgcccat gaactgatgg
     5281 ccgatctcga aaaagagacg gtcgatttcg ttgataacta tgacggcacg gaaaaaattc
     5341 ccgacgtcat gccaaccaaa attcctaacc tgctggtgaa cggttcttcc ggtatcgccg
     5401 taggtatggc aaccaacatc ccgccgcaca acctgacgga agtcatcaac ggttgtctgg
     5461 cgtatatcga tgatgaagac atcagcattg aagggctgat ggaacacatc ccggggccgg
     5521 acttcccgac ggcggcaatc attaacggtc gtcgcggtat tgaagaagct taccgtaccg
     5581 gtcgcggcaa ggtatatatc cgcgcccgtg ctgaagtgga agttgacgcc aaaaccggac
     5641 gtgaaaccat tatcgtccac gaaattccgt atcaggtaaa caaagcgcgc ctgatcgaga
     5701 agattgcgga actggtaaaa gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt
     5761 ctgacaaaga cggtatgcgc atcgtgattg aagtgaaacg cgatgcggtc ggtgaagttg
     5821 tgctcaacaa cctctactcc cagacccagt tgcaggtttc tttcggtatc aacatggtgg
     5881 cattgcacca tggtcagcca aagatcatga acctgaaaga catcatcgcg gcgtttgttc
     5941 gtcaccgccg tgaagtggtg acccgtcgta ctattttcga actgcgtaaa gctcgcgatc
     6001 gtgctcatat ccttgaagca ttagccgtgg cgctggcgaa catcgacccg atcatcgaac
     6061 tgatccgtca tgcgccgacg cctgcagaag cgaaaactgc gctggtggct aatccgtggc
     6121 agctgggcaa cgttgccgcg atgctcgaac gtgctggcga cgatgctgcg cgtccggaat
     6181 ggctggagcc agagttcggc gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc
     6241 aggcaattct ggatctgcgt ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg
     6301 acgaatacaa agagctgctg gatcagatcg cggaactgct gcgtattctt ggtagcgccg
     6361 atcgtctgat ggaagtgatc cgtgaagagc tggagctggt tcgtgaacag ttcggtgaca
     6421 aacgtcgtac tgaaatcacc gccaacagcg cagacatcaa cctggaagat ctgatcaccc
     6481 aggaagatgt ggtcgtgacg ctctctcacc agggctacgt taaatatcag ccgctttctg
     6541 aatacgaagc gcagcgtcgt ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag
     6601 actttatcga ccgactgctg gtggcgaaca cgcacgacca tattctgtgc ttctccagcc
     6661 gtggtcgcgt ctattcgatg aaagtttacc agttgccgga ggccactcgt ggcgcgcgcg
     6721 gtcgtccgat cgtcaacctg ctgccgctgg agcaggacga acgtatcacc gcgatcctgc
     6781 cggtgaccga gtttgaagaa ggcgtgaaag tcttcatggc gaccgctaac ggtactgtga
     6841 agaaaaccgt cctcaccgag ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac
     6901 tggttgaagg cgatgagctg atcggcgttg acctgaccag cggcgaagac gaagtaatgc
     6961 tgttctccgc cgaaggtaaa gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct
     7021 gcaacaccac cggtgttcgc ggtattcgtt taggcgaagg tgataaagtc gtctctctga
     7081 tcgtgcctcg tggcgatggc gcaatcctca ccgcaacgca gaacggttat ggtaaacgta
     7141 ccgcagtggc ggaatacccg actaagtcgc gtgcgacgaa aggggttatc tccatcaagg
     7201 ttaccgaacg taacggttta gttgttggcg cggtacaggt agatgactgc gaccagatca
     7261 tgatgatcac cgatgccggt acgctggtac gtactcgcgt ttcagaaatc agcatcgtgg
     7321 gccgtaacac ccagggcgtg atcctcatcc gtactgcgga agatgaaaac gtagtgggtc
     7381 tgcaacgtgt tgctgaaccg gttgacgagg aagatctgga taccatcgac ggcagtgccg
     7441 cggaagggga cgatgaaatc gctccggaag tggacgttga cgacgagcca gaagaagaat
     7501 aattttactt cttcatgcca aaagggagcc atctcccttt tttgaattga aaagtccagg
     7561 ctgcaaagtc tgggcttttg tcgtattagg gcgcggtaaa gtttggctgt gtccgtaaaa
     7621 aatggctggc tatacacaag gaatgtggca atgagtggtg aaaaaaagcg aaaggctggc
     7681 ggttctatgg tcttgtaggt tttggcgcaa tagcactgct ttccgctggc gtctgggcgt
     7741 tgcaatatgc tggcagtggg ccagaaaaaa cgttgtcgcc gctggtggtg cacaataatt
     7801 tgcaaattga tctcaatgag ccggacctct ttctcgacag cgattctcta agccagcttc
     7861 ccaaagatct cctcaccatt ccgtttctcc acgatgttct gagtgaagat ttcgttttct
     7921 attatcagaa tcatgccgat cgtctgggca ttgaaggcag cattcgtcgc attgtctatg
     7981 aacacgatct cacgctgaaa gataagctct tttcgtcact cttagatcag cccgcgcagg
     8041 cggcgctatg gcacgataaa caaggccatc tttcacatta catggtgctg atccaacgca
     8101 gtggtttaag caaactgctg gagccattgt tatttgccgc taccagcgac agccagttaa
     8161 gcaaaacgga aatcagtagc atcaagataa atagtgaaac tattcctgtt tatcagttgc
     8221 gctataacgg caataacgcc ctgatgttcg cgacatatca ggacaagatg ctggtgtttt
     8281 ccagcacgga tatgctgttt aaagatgatc agcaggatac cgaagccacg gcgatcgcaa
     8341 gtgatttgtt gagcggcaaa aaacgctggc aagccagctt tggcctggaa gagcgtgctg
     8401 ccgaaaaaac gccagtacgc cagcgcatcg tggccagcgc caggttgctg ggttttggct
     8461 accagcggtt aatgccttct tttgctggcg tacgcttcga aatgggtaac gacggctggc
     8521 acagttttgt ggcgttaaat gatgaatccg ccagcgtaga tgccagtttc gattttacgc
     8581 cggtctggaa cagtatgcct gccggggcca gcttctgtgt ggcggtgccg tattcacacg
     8641 gtattgccga agagatgctt tcgcacatca gccaggaaaa cgacaagttg aatggggcgt
     8701 tggacggtgc cgcggggctg tgctggtatg aagactcaaa attgcaaacc ccgctgtttg
     8761 tcggtcagtt tgatggcact gccgaacagg cgcaattgcc agggaaactg tttacgcaaa
     8821 atattggtgc gcacgaaagc aaagcgccag aaggtgtttt gccggtaagc cagactcagc
     8881 agggcgaagc gcaaatctgg cgtcgcgaag tgagttcccg atacggtcag tatccgaaag
     8941 cgcaggcggc gcaacccgat caattaatgt cggattattt tttccgcgtg tcgctggcga
     9001 tgcaaaacaa aacgctgctt ttctccctcg atgacacgct ggttaataac gccctgcaaa
     9061 cactgaataa aacccgcccg gcaatggtgg atgtaatacc cactgatggc atcgttccgc
     9121 tctatatcaa tccacaaggc gtggcgaaac tgctgcgtaa cgaaacgttg accagtctgc
     9181 cgaagaatct cgaaccggtt ttttataacg ccgcacaaac tttattaatg ccgaagctgg
     9241 acgctttatc tcaacaaccg cgttatgtca tgaagctggc ccagatggaa cccggtgccg
     9301 cctggcagtg gctacccata atctggcagc cgctatgagg cacgggctgc tggcgctgat
     9361 ttgctggctg tgttgtgttg ttgcccatag cgaaatgctg aatgtcgaac aatccgggct
     9421 gtttcgcgcc tggtttgtac gcattgcaca agaacagctc cgccaggggc caagtccacg
     9481 ctggtatcag caggattgtg cgggcctggt gcgatttgcg gcgaacgaga cgctgaaagt
     9541 tcacgacagt aaatggctaa aaagtaacgg tttatctagc cagtatttgc cgccagagat
     9601 gacgctaaca cctgaacagc gtcaactggc gcaaaactgg aatcagggga acgggaaaac
     9661 cggcccctac gtgaccgcga ttaatttgat tcagtacaac agccagttta ttggccagga
     9721 cataaaccag gcgctgcctg gcgatatgat ttttttcgat cagggcgatg cccagcactt
     9781 aatggtctgg atggggcgtt acgtcatcta ccacaccgga agcgccacga aaactgacaa
     9841 cggaatgcgc gcagtcagtc tgcaacaact tatgacatgg aaggacaccc gatggatacc
     9901 caacgattcc aatcccaatt tcattggcat ttatcgttta aattttctgg cgcgatagca
     9961 gcgtgcttgt ccctgtctct tgtgggaaca gggcttgcta atgctgatga ttcgcttcct
    10021 tccagtaact atgcgccgcc cgccggggga acattctttt tgcttgctga cagcagtttt
    10081 agcagcagtg aagagtcgaa agtgcgactg gaagcgccgg ggcgtgatta tcggcgctat
    10141 cagatggaag agtacggcgg cgtggacgtt cgcctgtatc gtattcctga cccgatggca
    10201 tttttgcgcc agcagaaaaa cctgcatcgc attgtggtgc aaccgcaata tctgggcgac
    10261 gggctgaaca atacgctgac ctggctgtgg gataactggt acggcaaatc tcgccgcgtg
    10321 atgcagcgta ctttctcttc tcagtcacgg cagaatgtga ctcaggcatt acccgaatta
    10381 cagctcggca atgccattat taaaccttcc cgttatgtac agaacaacca gttttccccg
    10441 ctgaaaaaat atcccctggt ggaacagttc cgttatccac tatggcaggc taaaccggtc
    10501 gagccgcagc aaggggtaaa actggaaggc gcatccagca atttcatctc gccgcagccg
    10561 ggtaacattt atattcctct cggccaacaa gagccgggac tgtacctcgt cgaggcgatg
    10621 gttggtgggt atcgggcgac gacggtggtg tttgtttccg ataccgtggc gcttagcaaa
    10681 gtgtcaggca aagagcttct ggtgtggacc gcgggtaaaa aacagggtga agcgaagccc
    10741 ggctcagaga tcttgtggac tgacggtctt ggcgtgatga cccgcggtgt gaccgatgac
    10801 agcggtacct tgcagttaca acatatatcg ccagaacgtt catacattct gggtaaggat
    10861 gctgaaggcg gcgtttttgt ctccgagaac ttcttctacg aaagcgaaat ctacaacacc
    10921 cgcttgtata tttttaccga tcgcccgcta tatcgcgcag gcgatcgtgt cgatgttaaa
    10981 gtgatgggcc gcgagttcca cgatccgttg cattcatccc ccatcgtcag cgccccggcg
    11041 aagctttcgg tgctggacgc taacggcagt ctgttgcaaa ccgtcgatgt cacgctggat
    11101 gcgcgcaatg gcgggcaggg aagtttccgc ctgccagaaa atgccgttgc cggaggttat
    11161 gagttacgtc ttgcttaccg caatcaggtc tatagcagca gttttcgcgt ggcaaactac
    11221 atcaagccac atttcgagat tggtttagct ctcgacaaaa aagagttcaa aactggcgaa
    11281 gcggtcagcg gcaaactgca acttctctac ccggatggcg agccggtaaa aaatgcccgc
    11341 gtgcagttaa gtttgcgcgc tcagcaatta tcaatggtcg gtaacgattt gcgttatgcc
    11401 ggacgtttcc ccgtgtcgct ggaaggcagc gaaacggtgt ccgacgccag cggtcatgtg
    11461 gcgttaaatc tccccgccgc cgataaaccg agccgctatt tgttaaccgt ctccgccagt
    11521 gacggcgcgg cgtatcgcgt caccaccacc aaagagatcc tcattgaacg cggcctggcg
    11581 cattactctt taagtaccgc cgcacaatac agtaatagcg gcgagtcggt tgtgttccgt
    11641 tatgccgcgc tggaatcttc aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa
    11701 gaccgcacga gccatagcgg agatctaccg tcaggcggca aatcctttac cgtcaatttc
    11761 gataaacctg gcaactacaa tctgacgtta cgcgataaag acggcttaat tctcgccggg
    11821 ttaagccatg ccgtcagcgg taagggcagt atgtcgcata ctggtacggt agatatcgtg
    11881 gcagataaaa cgctgtacca gcctggcgaa accgcgaaga tgctgattac cttcccggag
    11941 ccaattgatg aagcattatt gacgctggaa cgcgatcgcg ttgaacagca gtcgctgcta
    12001 tcgcatccgg caaactggct gacgttacaa cgtttaaacg atactcaata tgaagcccgt
    12061 gttccagtga gcaattcctt tgcgcctaac atcacttttt cggtgctgta tacccgtaac
    12121 ggtcagtaca gttttcagaa cgccgggatc aaagttgccg ttccccagct ggatatccgg
    12181 gtgaaaacgg acaaaaccca ttaccagcct ggtgaactgg tcaatgtcga attaacctcg
    12241 tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga tgaaatgatc
    12301 tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc gctggggcgt
    12361 aacaatgtgc gtaccagctc cagtctgtcg tttatcagct acgaccaggc actctccagc
    12421 gagccggttg cgcctggcgc aactaaccgc agcgagcggc gagtaaaaat gcttgaacgt
    12481 ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac cgataaacaa
    12541 ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg tatcaccgcg
    12601 cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg ttcggaaaaa
    12661 aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa accgtcggca
    12721 ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac taaatttgca
    12781 ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat ttcgctggcg
    12841 cagaacattc agcaatctgg cttgttaagc gcagaactgc aacaaaatgg gcaagtgcag
    12901 gacagcatta gcacaaaact gtcttttgtg gataacagct ggcccgttga acagcagaaa
    12961 aatgtcatgc tcggcggtgg cgataacgcg ctgatgttgc ccgagcaggc gagcaatatc
    13021 cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga tgcgttagtc
    13081 gatgaaccgt gggggggggt gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc
    13141 tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca gatgattcag
    13201 gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac ctggtggggt
    13261 gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga ctggcaggcc
    13321 agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct cgacagttat
    13381 gccgagcagg cagataacat gccgttattg catcgggcgc tggtgctggc gtgggcacag
    13441 gagatgaatc tgccgtgcaa aacgttgttg aaagggttgg atgaagctat cgcccggcgc
    13501 ggaactaaaa ctgaagattt ctctgaggaa gacacccgcg atatcaatga cagcctgatc
    13561 ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac catgacgttg
    13621 ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata tgcgtgggat
    13681 aaagcggtaa acagcaatca gccgctggca cacacggttg tgctgctcaa tagcgggggc
    13741 gacgctaccc aggcggctgc tattttaagt ggtttgaccg ctgagcaatc tactattgag
    13801 cgcgcgctgg ccatgaactg gctggcgaaa tatatggcga caatgccttc ggttgtgttg
    13861 cctgcgcctg cgggcgcatg ggccaaacat aagttaactg gagggggcga atactggcgt
    13921 tgggttggtc agggcgtgcc ggacattctc tcttttggtg acgaattatc gccgcaaaat
    13981 gtgcaggtcc gctggcgtga agcggcaaaa acggctcaac aaagtaacat tccggtgacc
    14041 gttgaacgcc aattgtatcg gcttatccct ggtgaagaag agatgagctt tactctgcaa
    14101 ccggtgacca gcaatgagat tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc
    14161 agcgagcagg atgcagttct gcgctatggt caggtggaag taccgctccc gccgggagcc
    14221 gacgttgagc gcacaacatg gggcatttca gtcaataaac ccaacgctgg aaaacagcag
    14281 gggcaattgc tggaaaaagc gcgaaatgaa atgggcgaac tggcctatat ggtgccggtg
    14341 aaagaactga cgggaacggt cactttccgc catttgctgc gcttctcgca aaaagggcaa
    14401 ttcgttctgc ctcctgctcg ttatgtgcgt tcctatgcac ctgcacagca aagtgttgcg
    14461 gcagggagcg aatggaccgg gatgcaggtg aaataagtga actggcgcag aatcgtctgg
    14521 ctattggcgt tggttactct gccaacgctt gcggaggaga cgccgttaca actggcgctg
    14581 cgcggtgcgc agcacgatca actgtatcag ctgtcgtcat caggcgtgac caaagtctca
    14641 gcattacctg acacgctcac cacgccgtta gggagtttat ggaaactgta tgtttacgcc
    14701 tggctggagg atacccatca accggagcag ccctaccagt gtcgcggaaa ctccccggaa
    14761 gaggtttatt gttgtcaggc tggggagagc ataacgcgcg ataccgcgct ggtacgttcc
    14821 tgtgggctct attttgcgcc gcagcggttg catattgggg cagatgtgtg ggggcaatac
    14881 tggcaacagc gtcaggcacc tgcgtggtta gcgtctcttt ctacgctgaa gccagaaacc
    14941 tcggtaacgg ttaaatcgct gcttgattca ttagcgacgt tacctgcgca aaacaaagct
    15001 caggaagtct tactggatgt ggtgctggac gaggcgaaaa tcggtgtcgc atctatgctc
    15061 ggtagtcggg tacgggtgaa aacctggagc tggtttgccg atgataaaca ggaaatccgt
    15121 cagggtggtt ttgctggctg gttaaccgat ggtacaccgc tgtgggtgac tggcagtggc
    15181 accagcaaaa cggtactgac ccgttatgcg acggtgctta atcgggtttt gccagtgccg
    15241 acgcaagttg ccagtgggca atgtgtagag gtggagctat ttgcgcgcta gccgctgaag
    15301 aaaatcacag cggagaagag tacgacggca gtaaaacccg gcgtacttaa tgggcgctat
    15361 cgtgtgacgt ttgccaatgg taaccacatt acgtttgtca gccacggtga gacgacctta
    15421 ctgaccgaaa agggcaaact gaaattgcag tcgcatctcg atcgtgaaga gtacgttgcc
    15481 agagttctgg atcgcgaggc gaaaagtacg ccacctgagg cagcgaaagc catgactgtg
    15541 gcgatacgca cttttttaca gcaaaacgcc aatcgggaag gcgattgcct gaccatcccc
    15601 gacagcagcg ccacgcagcg tgtttctgct tcgccagcta ccacgggggc gcgaacgatg
    15661 accgtctgga cgcaggatct catctatgcg ggcgatccgg ttcattatca cggcagtcgc
    15721 gctaccgaag ggacgctttc ctggcggcaa gccatggccc aggcggggaa gggagagcgt
    15781 tacgaccaga tactcgcgtt tgcttatccc gacaacagcc ttagccgctg gggagcgcca
    15841 cgcacaacct gtcagttatt acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag
    15901 tggcggcgta tattacaagg cgagacgggg tacaacgaac cagacgtgtt tgcggtctgt
    15961 cgtttagtct caggtttccc ctataccgat cggcagcaga aacggctgtt tattcgtaac
    16021 ttctttacgc ttcaggatcg gctggattta acccatgagt atctgcacct ggcctttgat
    16081 ggttatccca ccgggcttga tgagaactat atcgaaacgc tgacccgaca attattaatg
    16141 gactaatgct atgcgaaaga tttttcttcc gttgttactg gtggcgcttt cgcctgtcgc
    16201 tcacagtgaa ggtgtgcagg aagtcgagat tgacgcaccg ctctctggct ggcatccggc
    16261 agagggcgaa gatgccagtt tttcacaatc gatcaattac ccggcgtcgt ccgtcaacat
    16321 ggcagatgat caaaatatct cagcgcagat ccgcggcaaa ataaagaatt atgctgcggc
    16381 gggtaaagtt cagcagggcc ggctggtggt caacggtgcc agtatgccgc agagaattga
    16441 atccgatggt tcatttgcac gcccttatat tttcactgaa ggcagcaaca gcgtgcaggt
    16501 catcagcccg gatgggcaaa gccgacaaaa aatgcagttt tactcaacgc cgggtacagg
    16561 aacgattcgt gcacgtttac ggctggttct ctcgtgggat acggacaata ccgacctcga
    16621 tcttcacgtt gtcacgcccg atggcgaaca cgcctggtac ggtaacaccg tgctgaaaaa
    16681 cagtggtgca ttggatatgg atgtcacgac ggggtacgga cccgagattt tcgccatgcc
    16741 agcaccagtt cacgggcgct atcaggtgta tatcaactac tatggtgggc gcagcgaaac
    16801 ggaattaacc accgcccaac tgacgctgat caccgatgaa ggatcggtca atgagaaaca
    16861 ggaaacattt attgtaccga tgcgtaatgc tggcgagctg acgctggtga aaagttttga
    16921 ctggtgatcc atcccttcac acaacctggc tctttctttt ttcagataag actgctgggc
    16981 gatctgccga cgccaggata taaccattaa caagcgtcgg cagattggtc atttcttatt
    17041 tttcgtactg agaactgtcc atttactccg gctttcctgg ttaaggcaat ggtttctaag
    17101 cgatacgcat tattgcaatg gtgacagaac attgcggtaa cacgctttta ccgctacctt
    17161 aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt
    17221 ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta gtgctctggc
    17281 tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag cgagaatcgg
    17341 aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt caacgcacct
    17401 ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca gaaaacggtg
    17461 tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg
    17521 ccgactccga ttgttcagca atgagtaaca cctggcgagg ttctctggag tcattggcgt
    17581 ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctcaac cgggtatttt
    17641 taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg ccagtggaac
    17701 gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat gcaccacaag
    17761 atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc gtcgggtatt
    17821 tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg ggtgtcgagc
    17881 agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg gttaccattc
    17941 ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagcaaa attaaggccg
    18001 atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc cgggagctgg
    18061 tgctgaagaa aaatctgcca ccctcatcgc tcagcatcgt gtattcggtg ccggttgata
    18121 aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg
    18181 gagctgcatt gtttactctc gcgcggatgt acgagcgacg tattttcatt ccggcggaaa
    18241 gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc gcctccgcgc
    18301 cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt aacgaactgg
    18361 cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgaca caaattatct
    18421 gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat ctgcaaatca
    18481 gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt
    18541 cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg gaacaggcga
    18601 gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg ccgctgtatg
    18661 gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcgtc
    18721 tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc gatattctcg
    18781 atttctcgaa gattgaatcg gaacagttga tgatcgaacc gcgtgagttt tcaccgcgtg
    18841 aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag cagttaggct
    18901 tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg atgcgtttac
    18961 agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc ggctgtatag
    19021 ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat accggcgtgg
    19081 ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg
    19141 tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca
    19201 tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag tttaccgtgc
    19261 gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg ttgagtggta
    19321 aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa accagtttgc
    19381 agcgcagcgg catcgtcgta acaacatacg aagggcagga accgactccc gaagatgtgt
    19441 taatcactga cgaggtagtg agtaaaaaat ggcagggcag agcggtagtg accttctgtc
    19501 gtcgacatat tggtattccg ctggagaaag cgccagggga gtgggtacac agtgtggctg
    19561 ctccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg gagagcgacg
    19621 atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac gatatgatga
    19681 ttctggtcgt ggatgatcat ccgattaacc ggcgtttgct ggcagatcag ttgggatcgt
    19741 tgggctatca atgtaaaacc gcgaatgatg gcgtcgatgc gcttaatgta cttagcaaga
    19801 atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt taccgcttga
    19861 cacaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaacg gctaatgcgt
    19921 tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg tcgaagccag
    19981 taacgctgga tgttataaaa cagacgctga cggtatatgc cgagagggtc aggaaatcgc
    20041 gggaatcgta ggacggataa ggcgtttacg ccgcatctgg cattcagtgc aaatgccaga
    20101 tgcgacgctg acgcgtctta tctggcctac tttaatgtaa tctgttgaaa taatgggaat
    20161 cgtaggacgg ataaggcgtt tacgccgcat ccggcaatcg gtgcaaatgc cagataagac
    20221 actaacgcgt cttatctggc ctacagatga ttagtcttta tctgccggac ttaacgttac
    20281 tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca tcatcgcaga
    20341 tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga tctcggtcac
    20401 caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga gacgtttgtc
    20461 accgtaacct ccagcactga ttttttccaa caggcgagaa acgctttccg gggtaaattt
    20521 ctttcctttt tgcagcgctg cgagggcttt cggcagatcg gtcggtgcac cttgtttcag
    20581 cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt tgttgttcat
    20641 ggtcagaaca ataatcgaca ggcttgggaa atggcgcttg atgtacttaa ttaaggtaat
    20701 gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat gcgcatccag
    20761 tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca
    20821 ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt catcggcaat
    20881 aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc ttgacataac
    20941 tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga atcagatgtt
    21001 ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat acgcctttaa
    21061 gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca gtatacagcc
    21121 tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg gcatggagtt
    21181 gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt
    21241 caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg acgcacaatt
    21301 gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag ccagaggcag
    21361 taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt aatctttcat
    21421 cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata ttccgaattt
    21481 ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct tcttcctctt
    21541 caacttccgg gtcagctgcg agcattttga tatgcacaga gtagcgtgta ccaagcccat
    21601 cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg ctcagccaga
    21661 atgccagcgg gtccgccttg ccatagcgat cgttttgggt ctggttgata aacgggaagt
    21721 gcaaattatc catttcatga atgcttacgc cttctccggt gtccagaatg cggaacgtca
    21781 ggcggtcttc ggaggactca tcctgatcaa cctcaagggt gatttttccc aattgcgttg
    21841 aggtcacggc atattgcatc agtagcagca aaatacgtcg taatgcatcg cgatcgccgc
    21901 ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct ttacgcttga
    21961 tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag aacagcaccg
    22021 tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca tcgaccaatc
    22081 gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg agtttagccg
    22141 cgctctccgc cagggactgt gcgggttctt tcagcgcatc gccaatgttt ttcataaagg
    22201 tcatccgccc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt ttcttgttta
    22261 ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc gcaacctggc
    22321 tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata atcccctgat
    22381 gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa tgatcggcaa
    22441 ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg ccgagcggca
    22501 gcagtgagac tatctcttca ttgattgccc gtaaaatgcg caattcgtta ttgaccgccg
    22561 tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta tagccaaata
    22621 acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt tgcaacgtat
    22681 ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta gagttgagtg
    22741 ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc gttccttctt
    22801 tctcattatc attgtttccc gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg
    22861 gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc gttgccagat
    22921 gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc caggccaggc
    22981 ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc aacatctctg
    23041 cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag gtgagatctt
    23101 tgaggggcag ggttgagatc agcaccagac tgttatcctg accattcagg taatacattg
    23161 accacggtac attttctgcg ccccacaatg tgtccagata ggtggacatc cgttgagtca
    23221 tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg cggcgcggtt
    23281 tttccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg
    23341 gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga
    23401 cgcgcttttg gatagcgtgg gtaatgtcga caatttggta acttttatct ttcagccagg
    23461 cgttaacggc gctttgtacc attacaccca tcgtcaccag taacacaatg atcaacagta
    23521 aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt
    23581 tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg cagtataaag
    23641 ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct cccgtgatat
    23701 ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta caaataacct
    23761 acaaaaagcc caacgaaaat aattgcaaat aaacaaagat tgctggaaat tatgctgatg
    23821 ttaattattt gtgaaatagt taacaagcgt tatagttttt ctgtggtagc acagaataat
    23881 gaaaagtgtg taaagaaggg taaaaaaaac cggatgcgag gcatccggtt gaaatagggg
    23941 taaacagaca ttcagaaatg aatgacggta ataaataaag ttaatgatga tagcggtagt
    24001 tattctagtt gcgagt
//
LOCUS       DAZKFZ010000058        23859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000058 DAZKFZ010000000
VERSION     DAZKFZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(76..1341)
                     /locus_tag="ACHOQW_002082"
     CDS             complement(76..1341)
                     /locus_tag="ACHOQW_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9573163.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ACHOQW_002083"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHOQW_002083"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHOQW_002084"
     CDS             complement(1720..2427)
                     /gene="yqgA"
                     /locus_tag="ACHOQW_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9573164.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2580..2663
                     /locus_tag="ACHOQW_002085"
     CDS             2580..2663
                     /locus_tag="ACHOQW_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573165.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2825..4960
                     /gene="speC"
                     /locus_tag="ACHOQW_002086"
     CDS             2825..4960
                     /gene="speC"
                     /locus_tag="ACHOQW_002086"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP9573166.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHOQW_002087"
     CDS             complement(5010..6266)
                     /gene="nupG"
                     /locus_tag="ACHOQW_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP9573167.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHOQW_002088"
     CDS             complement(6468..7547)
                     /gene="mltC"
                     /locus_tag="ACHOQW_002088"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP9573168.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHOQW_002089"
     CDS             complement(7612..7887)
                     /gene="yggX"
                     /locus_tag="ACHOQW_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP9573169.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHOQW_002090"
     CDS             complement(7915..8967)
                     /gene="mutY"
                     /locus_tag="ACHOQW_002090"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9573170.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9128..9847
                     /gene="trmB"
                     /locus_tag="ACHOQW_002091"
     CDS             9128..9847
                     /gene="trmB"
                     /locus_tag="ACHOQW_002091"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9573171.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9847..10173
                     /gene="yggL"
                     /locus_tag="ACHOQW_002092"
     CDS             9847..10173
                     /gene="yggL"
                     /locus_tag="ACHOQW_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP9573172.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10357..11076
                     /gene="yggN"
                     /locus_tag="ACHOQW_002093"
     CDS             10357..11076
                     /gene="yggN"
                     /locus_tag="ACHOQW_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP9573173.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11252..12298
                     /gene="ansB"
                     /locus_tag="ACHOQW_002094"
     CDS             11252..12298
                     /gene="ansB"
                     /locus_tag="ACHOQW_002094"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP9573174.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12415..13422
                     /gene="yggM"
                     /locus_tag="ACHOQW_002095"
     CDS             12415..13422
                     /gene="yggM"
                     /locus_tag="ACHOQW_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP9573175.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ACHOQW_002096"
     CDS             complement(13665..14801)
                     /gene="hemW"
                     /locus_tag="ACHOQW_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9573176.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ACHOQW_002097"
     CDS             complement(14794..15387)
                     /gene="rdgB"
                     /locus_tag="ACHOQW_002097"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP9573177.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ACHOQW_002098"
     CDS             complement(15395..15685)
                     /gene="yggU"
                     /locus_tag="ACHOQW_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP9573178.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ACHOQW_002099"
     CDS             complement(15682..16248)
                     /gene="yggT"
                     /locus_tag="ACHOQW_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP9573179.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ACHOQW_002100"
     CDS             complement(16266..16970)
                     /gene="yggS"
                     /locus_tag="ACHOQW_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP9573180.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16988..17968
                     /gene="yggR"
                     /locus_tag="ACHOQW_002101"
     CDS             16988..17968
                     /gene="yggR"
                     /locus_tag="ACHOQW_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP9573181.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ACHOQW_002102"
     CDS             complement(18159..18575)
                     /gene="ruvX"
                     /locus_tag="ACHOQW_002102"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9573182.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ACHOQW_002103"
     CDS             complement(18575..19138)
                     /gene="yqgE"
                     /locus_tag="ACHOQW_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP9573183.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ACHOQW_002104"
     CDS             complement(19250..20197)
                     /gene="gshB"
                     /locus_tag="ACHOQW_002104"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP9573184.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ACHOQW_002105"
     CDS             complement(20210..20941)
                     /gene="rsmE"
                     /locus_tag="ACHOQW_002105"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9573185.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ACHOQW_002106"
     CDS             complement(21021..21728)
                     /gene="endA"
                     /locus_tag="ACHOQW_002106"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP9573186.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ACHOQW_002107"
     CDS             complement(21823..22320)
                     /gene="yggI"
                     /locus_tag="ACHOQW_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP9573187.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ACHOQW_002108"
     CDS             complement(22397..23791)
                     /gene="galP"
                     /locus_tag="ACHOQW_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP9573188.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5869 a         6005 c         6259 g         5726 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggtcca
     1741 ggcggcggaa agcggcattg ccagaaggag tgctggaagc atgttgacca ccgggaacat
     1801 tttaatgcca cagatgcgta atccggttgc cagcagcaat aaaccgccta cggcgctgaa
     1861 gtctgccatc atcgacggtg tggtcagcgg taatatcagc gcggcagccc acgccagcgt
     1921 taactggatg atcagtaatg ggatactaat caccgatacc gcaataccca gcgagcaggc
     1981 gaagatcatc gccgtaaaga aatcaagaaa tgacttggcg attaaaatac tcggatcgcc
     2041 ggtcatccct tcgttcatcg ccccgaagat cccggtgccg ctggcgcaaa acaggacaat
     2101 aatcgcgaca taattctgaa taaaagattc atgcgctggc ttcttacgtg agtgacgaaa
     2161 cagattttgt gctttggcga ccgctgtatt gacgcctttc tccagcagac aaatttcgcc
     2221 gattaatgct ccgagtaagg ttgctaaaac catcgccgga aggttggcgc atttcaccac
     2281 cagtaaaata ccaatcccca gcgatgccaa accaaaaatt gatgtcatgg agacgcggat
     2341 acgttccggt aagcgttggc taagcagtgc accgagcatg ccacccagta atattgcact
     2401 tgcgttgata aaagggccga tgaccacaag agttcctgtt aactgtattg tttgattttc
     2461 ttattatgaa ctttttgcct tgttgatggc ttgtctggtc gcaaagtcct ggcttgcacg
     2521 ccttagcgaa aggtgccatg attgcgcgaa ttttctcctc tctgtacgga gtttgcccga
     2581 tgcacgccat ctccttacat tctctcgctt atcgccgttt cgcgcgaaac ccttctcttt
     2641 ttatgctact gctcatgcgg tgaagttaac gcacgctcac tgcaggacaa cagtaaaatc
     2701 agagcctttc tgcttttact gatgtctggc ggtcggagct ggtgaccagt ttgacccata
     2761 tctcatgggg cagggtttta caccttgtcc agtattctta cttccccgaa acgggtttgc
     2821 gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac tttctactca
     2881 tcgccgcgtg gtggcgttgg gagatactga ttttacggac gtcgcggcag tcgtcattac
     2941 cgctgcggat agtcgcagtg gcattcttac gttgcttaag cgcaccggtt ttcatctacc
     3001 ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg cggtaatcaa
     3061 cggcaacgag cagcagtggc tggagctgga atccgcagcc tgtcagtatg aagagaattt
     3121 gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggcaaca gcacctttgc
     3181 ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac gccattttta
     3241 cgatttcttt ggtgagaaca tctttcgcgc cgatatgtgt aacgctgacg taaaattggg
     3301 cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaattcgcag ccaaagtctt
     3361 tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata aagtggtgac
     3421 gaatgcgctg ttaacgcgtg gcgatctggt gctctttgac cgtaacaacc ataagtcgaa
     3481 tcatcacggc gcgttgattc aggcgggggc gacgccggtc tatctggaag cttcacgtaa
     3541 cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt atctgcgcca
     3601 gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc gcctggcgat
     3661 tattcagctg ggaacctatg acggcactgt ctataacgcc cgtcaggtga tcgataccgt
     3721 tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttatg aacaatttat
     3781 cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg atccggggat
     3841 ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt cgcagatcca
     3901 taaaaaagat aaccatatcc gcggacaggc gcgtttttgc ccgcataagc ggttgaataa
     3961 cgcctttatg ctccatgctt ctaccagccc tttctatccg ctgtttgctg cactggatgt
     4021 taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt gcgttgagtt
     4081 gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc cgtttatccc
     4141 gcccgttgtt gatggcaaat tgtggcagga ttatccgaca tcagtgttag ccagcgaccg
     4201 tcgttttttc agttttgagc cgggggcgaa gtggcacggc tttgaaggat atgccgcgga
     4261 tcagtatttt gttgatccgt gcaagctgtt actcactaca ccaggtatcg atgccgaaac
     4321 cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactatc tgcgtgagaa
     4381 cggcattgtg ccggagaagt gcgatctcaa ttccattctg ttcttattaa ctccggcgga
     4441 aagccacgag aagctggcac aactggtggc gatgctggct caatttgaac agcatattga
     4501 ggatgactcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc cggtgcgcta
     4561 tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatctgtatg tcagtttcga
     4621 cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag tggtgatgaa
     4681 cccccaggat gcgcatagcg cttatattcg cggtgacgtg gagttggtgc ggattcgtga
     4741 tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg tgctttgcgt
     4801 ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcac tggaagaagg
     4861 ggtgaatttg ttgccgggat tttcgccgga gctgcaaggt gtttatagcg aaaccgatgc
     4921 ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa aacgggtcgc
     4981 cttctggcga cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt acctgtcggg
     5041 acacgaacgt gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggagtaa
     5101 ccagcgaaaa tcaaccatac ggtctgccag tcggtaatac cgttttgggt gtacatctca
     5161 acaactttac cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg
     5221 aacatccctt gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa
     5281 ccagagatgt tgaagaagtc gaacgcacag ccgtaaacaa tcatcgatag aaccagcagt
     5341 acggtgccga acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc
     5401 acaatactga tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc
     5461 agaatgaaca gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcaca
     5521 ataaagctgc tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac
     5581 atgttggtaa tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata
     5641 cgcttgtttt taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc
     5701 gcttgctgtt tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg
     5761 gaaagtgctg cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc
     5821 acccacattg ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta
     5881 acgatatcca tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc
     5941 gttggcatat aagcaaacga gttaatcaat atcacaagga acatcgcctc cggcgtcgtg
     6001 acctgtgccg ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat
     6061 ttcgcactta accatttgtc ggccacaatc cccagcagcg caggcataaa gaccgcggcg
     6121 atacccagtg agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac
     6181 atataggaac cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg
     6241 attttcagct gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt
     6301 ttgaaaaagc acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt
     6361 ttttgttacc tgtggcaaat aattgcaaac attcctaatt tgcaaaacga aattgtgata
     6421 tggataacat ttttgcccct gagcatcgtc aggggcggtt agtggaatta tcggcggcgg
     6481 taggattttt gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg
     6541 cgggtcgtca gcgtttgata aacatcgccc ggcgtcatgg tgttaataat attagccgcc
     6601 tgaatcttat cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg
     6661 atgacggcat aacgccggga tgtagggtta tcaattccgc cgagataaac attgttcagc
     6721 atcgccagat acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg
     6781 ctcggcgtgc cggatttccc ctgcgagcgg aacacatctt tcccggcagt atgttgtacc
     6841 acctgcatta accccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca
     6901 gtctgcataa ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg
     6961 accatgccga gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg
     7021 acgctgtaga tgatacgcag cccgttgctg cggctcttca gacggttttt cagcagataa
     7081 tccgcgaagt tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc
     7141 tgaccgtaaa ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg
     7201 accgaactcg gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg
     7261 gcaggttctg tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg
     7321 ctgcgggtct gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc
     7381 tctttgaatc cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa
     7441 ccgttggtgt ctttgaccca ggcttcgtta taggtaccgc cttttttggt cgtcgaacag
     7501 gagatgagca acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg
     7561 tgttgtgttt gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt
     7621 atcttccggc gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat
     7681 ctcctgctcc agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat
     7741 cagcatggtt tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat
     7801 gcgttttccc agttcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag
     7861 gaaagtacaa aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac
     7921 cggcgcgcca gtgcgtaact gctgtaacaa acgctccacg ggagccgcca ggccaactga
     7981 cggcggttgc gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga
     8041 cgacacggga agccacatag gcacaatatc taagtggaaa tggctgaagg tatgccgaaa
     8101 cgcggtcagc tgcgtcaggt tatcggcagc aatctgccgt tgcgccagcc actgccgcaa
     8161 actttcttcg tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg
     8221 acgctgcgcc agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc
     8281 cggcagcgtc tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc
     8341 aatacatccg ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat
     8401 cgcgcccaaa tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg
     8461 ctcgcttaaa ctccataatt tattctcgac ctcttttttc ccaggccagc cgcttacagc
     8521 atagcagcgc gccagcaccc gtttgacgtt accgtcgaga atcggaaagt gcttacccag
     8581 agaaagcgat agaatcgcgc ctgcggtgga acgcccgacg cccggcagtg ctgcaacttc
     8641 ctcaaaggtt tccgggaatt taccgccgtg taaggtcgcc acttgttgtg ccgctttatg
     8701 cagattgcgc gcgcgggcgt aatagccaag ccccgtccac aagtggagaa cttcgtcgag
     8761 cggcgcattg gcgagatcgg tcaccgtcgg gaagcgcgcc ataaagcgtt caaaataggg
     8821 gataacggtc gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg
     8881 cgtcttgtca atttgccagg gcagagtttt tcgcccgtat ttatcgtacc agtccagaac
     8941 ctgggctgaa aattgcgacg cttgcatggt caccgaattc actgttgttg ggggcaagat
     9001 tgcagcacag cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc
     9061 ttgcatccgg caactaactt tggataatgc ccgttttcag aacactttca caagcgacta
     9121 aatctttatg aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg
     9181 ccgtatccgt agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct
     9241 ggaaaactac tggccggtga tgggcgttga gttcagcgaa gatatgctgg atttccccgc
     9301 gctttttggc cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct
     9361 ggtggcaatg gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcgcc
     9421 gggcgttggt gcgtgcctgg cttctgcgca tgaagagggc ttaagcaacc tgcgcgtgat
     9481 gtgtcacgat gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt
     9541 gcagctcttt ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca
     9601 ggtgccgttt gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc
     9661 gaccgactgg gaaccttatg cggaacatat gattgaagtg atgtcttcta ttgacggtta
     9721 taaaaacctg tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa
     9781 atttgaacaa cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt
     9841 gaaataatgg caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc
     9901 caggaattag gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt
     9961 gataaaaccg ttgatgattt tattaacgag gttatcgaac cgaacaaact ggcctttgac
    10021 ggcagcggtt atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc
    10081 gaagaacatc aggcgattgt gcgtaagtgg ctggaagagc gcaacctgga tgaggtacgc
    10141 accagcgaac ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca
    10201 aaactgcctg atgcgctacg cttatcatgc ctacaaagat gcacgatcga gtaggcggga
    10261 taaggcgttt acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca
    10321 gcaaatgctg gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg
    10381 gcagcacttt cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg
    10441 cgtgacgatg tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg
    10501 gtgatcacgc cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc
    10561 cagcgcgagc aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat
    10621 gaaggcgcga aaagccgcgt cgagaaagcc cgtattgctc tggataaaat tatcgttcag
    10681 gagatgggcg aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa
    10741 gagcagatga accgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc
    10801 attgatcagg ttcgcgctga aggccagcaa ttagtgaatc aggcaatggg cggaatttta
    10861 caggacagca ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta
    10921 cagaatgtgc tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag
    10981 caggaaaaag atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa
    11041 gatagccgca aagccctggt cgggaattta aaataatcct ctattttaag acggcataat
    11101 acttttttat gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca
    11161 aaatatattc acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa
    11221 tgcgtagcgt tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc
    11281 cgcactggtt atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc
    11341 aaccggcggg accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg
    11401 taaagttggc gtagaaaatc tggttaatgc ggtgccgcaa ctgaaggaca ttgcgaacgt
    11461 taaaggcgag caggtagtga atatcggctc ccaggacatg aacgatgatg tctggctgac
    11521 actggcgaaa aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttactcacgg
    11581 taccgacacg atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc
    11641 ggtggtgatg gtcggcgcaa tgcgtccgtc cacgtctatg agcgcagacg gtccattcaa
    11701 cctgtataac gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt
    11761 ggtgatgaac gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt
    11821 agcgaccttc aagtctgtta actacggtcc gctgggatac attcacaacg gtaagattga
    11881 ctaccaacgt accccggcac gtaagcacac cagcgacacg ccgttcgatg tctctaagct
    11941 gaatgaactg ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc
    12001 taaagccctg gtagatgcgg gctatgatgg catcgtgagc gctggtgtgg gtaacggcaa
    12061 cctgtataaa tccgtgtttg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt
    12121 gcgttcttcc cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa
    12181 atacggtttt attgcctctg gtacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcaact
    12241 ggctctgacg caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc
    12301 atttcgcccc ggtatcgcgc cggggctttt tcacttcaga cttacgtcca ttgccaattt
    12361 taattgccct aatgataatc accagaataa attattccgc gcgagggttt tcgggtgaaa
    12421 aagcaatgga ttgttggcac ggcgctgctt atgttgatga ctggtaatgt ctgggcagat
    12481 ggcgaaccgc caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt
    12541 ctcaagcttg atgccatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg
    12601 tcagcggaag gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca
    12661 gattacgaac tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg
    12721 ttaaccagta aaggaacgcc cgcgtctggc tggacggtga acttttactc ttttcaggcg
    12781 gcagctagcg atcgtggtcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc
    12841 gtgaatagcg aagatttcaa ttatcgcttt agtcagcttg agactgcgtt gaatacccag
    12901 aagaactcga ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg
    12961 gcgcagaaag cagcggatgc atattgggga aaagatgcaa atggtaagca aatgacccgc
    13021 gaagatgcct ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc
    13081 gaggcgttcg cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat
    13141 aaacagagtg aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac
    13201 gaacaacggc gacagacttt tctgcaatca caaaaactta gccgtaaatt gcaggatgac
    13261 tggataacgc tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt
    13321 aaaaaagtcg cgattttgat gaaaattgac gatatcaacc aggctaatga acgctggaaa
    13381 aaagataccg aacaactgcg gcgcaatggg gtgattaagt aagttttgat atagcgcacg
    13441 atttgtagca ggatgcggcg ttgaccccgc atccggcaat caacgtctga tgcgacgcta
    13501 gcgcgtctta tcagacctac aacagctgcc acatgtcggc tgaggtgctc acgccgcatc
    13561 cggcaatcaa cgtctgatgc gacgcttgcg cgtcttatca gacctacaac cgttgccaca
    13621 tgtaggctgg ataaggcgtt cacgccgcat ccggcaatac aagtttactc agccagaaaa
    13681 agctccagca gcgaatttaa aaacagcttc ccatgttccg taatctgcca gtaatccgca
    13741 cattcggtga gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg
    13801 caaagcccgg tatacgcact aaactccacg cgcggcgcag gttccagcaa ccggaagcga
    13861 ttcataaaga actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac
    13921 cttccttgca taaaaccacg cggatgacgc gttttggtgg tacgcagaat gcgcccatcc
    13981 gggaaggtca ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaagcgccag
    14041 tagttgagat tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc
    14101 tgataacccg ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtcg
    14161 tcgtccggca gtaccggcgg gcgtgaacca aacagcgtat taggttcgat ggtcagttga
    14221 taccaggaaa gatgcggcgg attcagttca atagcctggc gcagatcgcc aagcgcctct
    14281 tccagcgatt gatccggtag cccatgcatc aaatcaaggt taaagctacg taaccctaaa
    14341 ccgctcgcca gctttgccgc tcgtttcgct tcttgcgggc catgaatacg tcccagccgt
    14401 ttcagctttt cttcgctaaa actctgtacc ccaatagaga tacgatttac accggcacgc
    14461 tgataatcaa caaagcgatc ggcttctacc gtaccggggt tcgcttccat ggtaatttct
    14521 gcatccgctg ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccgag
    14581 ccggaaagca ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga
    14641 gcgtaagcca catcgttgtc cagatcgtta agcagatgct gaacgtaatc gtcgtgcggc
    14701 acttctcctt tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac
    14761 gggatgtgaa tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccagcag
    14821 cagtttcaat gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc
    14881 agcggttttc ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc
    14941 cgccggttcg cgagtaatca cacccggcca gctgccatgg cacaccagcg gggtcggatc
    15001 ttctgcgtga cgcagataca ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg
    15061 tacgtctttc agtgtttcca gcagtttttg cagattcttt tgatcggtcg cgtcttcacc
    15121 ggaataacgt gcggagtaaa tacccggcgc gccgccaagc acatctaccg ccaggccaga
    15181 gtcgtcggca attgccggta aaccggtcac ttttgccgcg tggcgcgctt tcagaatcgc
    15241 gttttcgata aaggtcaggc cggtttcttc agcggaatca acgccgagtt ctgtttgggc
    15301 cacgatatca agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt
    15361 gccggttgcg aggacaactt tttgcatagg atacctaatt aattaacgcc gcgacttctg
    15421 gcgggatttg ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt
    15481 caatcaccac ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatggc
    15541 tgttggcctg accgtcaacc ggcggcgcgg taatggcgac ttttacctcg tcgccatgta
    15601 aaccgacaat agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt
    15661 catcgttaac tgttacggca ctcataacgc catccacagc cccggcagca gcatatttcc
    15721 ggtcgcctgt aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat
    15781 catcggcgag aaatcaatcc cccccattgc cggtagcaag cggcgaatcg ggcgcagcag
    15841 cggatcggcc agctgaatca gcacgtattc aatcgggcta cggccctggc ttacccagct
    15901 cataatcgcc atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat
    15961 cagtaaaccg gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc
    16021 tttgataaaa ctgagaatat aggcaaccag cagcgaggcg ctatcaatgg gtcccattgc
    16081 cggaataacg cggcgcagtg gcccgataat tggctgcgtt accttcacta caaactgtga
    16141 gaaggggttg taaaaatcac aatgagccca ctgcatccag atgcgtaata acagcaccat
    16201 ggtatacagc tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta
    16261 attccttatt ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc
    16321 gtgctacctg ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg
    16381 atatgcgggt agcgcgtttt cagtccggca aatgctacag ccatttggcg tgcaacttca
    16441 aactgcctta catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa
    16501 cgcggtagtt cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac
    16561 ttactgtttt catcactaat attaatttga atcagtacgt taagaggggg aagttctgcc
    16621 gggcgctgat cgttgaggcg ggtagcaatg cgcaaacggt cgatggtatg acaccagtcg
    16681 aaatgctctg ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct
    16741 aatcctgtta cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag
    16801 ttttcaccaa attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt
    16861 gttttactga ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc
    16921 gctgagattt tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga
    16981 tcttagtatg aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct
    17041 acacctgtgc agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc
    17101 gtttgatgcg ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc
    17161 aatattgctg gagaatggtc agctggattt tgccgtgtcg ctgacggaaa accagcgatt
    17221 gcgtggcagc gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc
    17281 gcactgcccg cagctcgaac agcttggcac accaccgata ttgccggagt tactcaagag
    17341 cgagaatggc ttgattctgg tgacgggggc gacggggagt ggcaaatcta ccacgctggc
    17401 ggcgatggtt ggttatctca atcaacatgc tgatgcgcat attctgacgc tggaagatcc
    17461 tgtggaatat ctctatgcca gccagcgatg tttgatccag cagcgggaaa tcggtttgca
    17521 ttgtatgacg ttcgcatcgg gattgcgggc cgcattgcgg gaagatcctg atgtgatttt
    17581 gctcggagag ctgcgtgaca gcgagacaat ccgtctggcg ctgacggcgg cagaaaccgg
    17641 gcatctggtg ctggcaacct tacatacacg tggtgccgcg caggcagttg agcgacttgt
    17701 ggattcattt ccggcgcagg aaaaagaccc cgtgcgtaat caactggcag gtagtttacg
    17761 ggcagtgctg tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctatttga
    17821 attgctgatt aacacacccg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt
    17881 accgcatgtt attcaaactg ggcaacaggt ggggatgata acgtttcagc agagttatca
    17941 gcagcgggtg ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gcttatcggg
    18001 cctacaaagg caataaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgc
    18061 tgccacatgt aggccggata aggcgttcac gccgcatccg gcaataaggc gtaaggcgcg
    18121 atgatattat ttacactcca gcaggcgatt aaatctcctt aatatccctg ctcgaaatag
    18181 ctttcgagaa taataaccgc agaggcagag tcgattttgc ctttgttgag cgcccgatag
    18241 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    18301 tttacttcaa caccgaaacg accatgaata cggttagcaa atttacgcgc tctggcggtt
    18361 aacggctgtt cggtgccgtc catattcagc ggtaaaccga cgatgatttc gtctggctgc
    18421 cactctttca gtaaacgttc gatgaggttc cagtccggcg taccgtcctg tgctttaatt
    18481 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    18541 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    18601 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaattggcg
    18661 ttttgaacag aatattcaga tctgccgggg cggttagcca cgcgttatcg agaatttctt
    18721 gttccagttg acctttttcc caggaggcat agcccagagc caccaatacg tcagacggtt
    18781 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    18841 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    18901 cagccagtgg gccgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    18961 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    19021 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    19081 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    19141 tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    19201 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tactgctgta
    19261 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    19321 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    19381 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt
    19441 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    19501 gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg tactggttcg ccgtccacca
    19561 ccagcacgcg tttgtcgcca tctttaatgg ctggcagata attttgtgcc atgcagtagc
    19621 gagtgccgtg ttcggtcaag gtttcggcaa tgacgccgag gtttggatcg ccttctttca
    19681 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    19741 tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt
    19801 cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa
    19861 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    19921 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    19981 gttcaccgac gaatgaaaac cactcttcgt agttctgttt cacgttcagc gtgcgggtat
    20041 gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt
    20101 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    20161 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    20221 cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa
    20281 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    20341 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aaaggcaacg
    20401 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    20461 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cgaccacact
    20521 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    20581 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    20641 cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    20701 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    20761 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    20821 gcaacgcctg tcccggcccc atacgcagta cgcgcccaat atggttggcg gcatcttcgc
    20881 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    20941 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    21001 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    21061 ttatgattgc cctgtacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc
    21121 ggatacatct tgtcccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg
    21181 taatggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    21241 tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    21301 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc gactgacggt
    21361 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    21421 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcggg aacgacgtgt
    21481 tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc gcacgattgc
    21541 agatcgacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    21601 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    21661 tcggccaacg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    21721 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    21781 tgcaatcagc cagatcagga aagttcctga tagttcagat ggttattttg cgaccagttg
    21841 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    21901 tacggtaagc tgatgttcct gacacttaca gctgtagggg aaggtgttgc ggcgcacgga
    21961 ttgcagttcg aactgatgcg tacggcgggc tggaacgccc agcacacttt ccatcatcca
    22021 cttccactct ttgccatgtg gtgctacgcg gccaaaatgt ttccatacca gcaaatgtgc
    22081 cagttcgtgc ggtaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    22141 attaaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    22201 gtaagagagt tttggctccg ggtagttacg ccccaatttc aggttggcct gggcgagttt
    22261 ttcccgcagg cgacgcataa cggcctgctg gatggcgata gggagacggg atgttttcat
    22321 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccacgacggg
    22381 aggaagcctg gggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    22441 gattacgttc aatatgttcc agcgaaacgt gtttggtttc tggcaccagc cacagtgtca
    22501 gcaggataaa caggacgttc agacccgcgt aaacccagaa ggtattagcg ttgcccagcg
    22561 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg
    22621 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    22681 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    22741 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    22801 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    22861 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    22921 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    22981 tgatgacgtt catcccggtg aattgctgca ttacctgtaa cagtacgcca aggaacaccg
    23041 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    23101 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    23161 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    23221 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    23281 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgattg
    23341 tgatcattaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttcag
    23401 agaggtacag tggtgcggta taagaggcca cacctaccgc cagccccagc agaacgcggg
    23461 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca
    23521 aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    23581 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    23641 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    23701 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    23761 ttgactgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    23821 gtgaattaag ataggtgagt acgacgtaaa aagatgtga
//
LOCUS       DAZKFZ010000059        23755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000059 DAZKFZ010000000
VERSION     DAZKFZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..692
                     /locus_tag="ACHOQW_002109"
     CDS             <1..692
                     /locus_tag="ACHOQW_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFP9573189.1"
                     /translation="LSLLDLDLFRGISSNYRVVVQLLISSCLSGDSDTIYDIQEELPF
                     SWGWIPVSIWKDVFQKCWTYLEKQINDKTLALHILQPFIAFMNHRAHIDRRLAPIANM
                     LLTYSESLPTGCDVLPTVSREQFNEAKQMLLRNPDSFGRISIFPKELWSSAITPELKS
                     VFNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQLSALFEFHYQQAPQQLGVIY
                     QYYFEQAGVCH"
     gene            692..7006
                     /locus_tag="ACHOQW_002110"
     CDS             692..7006
                     /locus_tag="ACHOQW_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9573190.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            7230..10088
                     /locus_tag="ACHOQW_002111"
     CDS             7230..10088
                     /locus_tag="ACHOQW_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9573191.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            10091..15025
                     /locus_tag="ACHOQW_002112"
     CDS             10091..15025
                     /locus_tag="ACHOQW_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573192.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNPRQKALLYCLVLSL
                     NSVNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            15025..21366
                     /locus_tag="ACHOQW_002113"
     CDS             15025..21366
                     /locus_tag="ACHOQW_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9573193.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            21363..23477
                     /locus_tag="ACHOQW_002114"
     CDS             21363..23477
                     /locus_tag="ACHOQW_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9573194.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         6017 a         5638 c         6348 g         5752 t
ORIGIN      
        1 gcttatcctt gctggatctg gacttgttca gaggtatttc gagtaattac cgcgtggttg
       61 ttcaattgtt aatatcatca tgtctttctg gtgatagcga tacgatttat gatatacagg
      121 aagaattacc cttttcatgg ggatggattc ccgtttcaat ctggaaagat gttttccaaa
      181 aatgttggac ttatctggag aaacagatta acgataaaac attagcatta catatattgc
      241 aaccctttat tgcttttatg aaccatcgtg cacatatcga tcgtcggctg gctccgattg
      301 cgaatatgtt acttacatat agtgagagcc taccaaccgg ttgtgatgta ttgccaactg
      361 ttagtcgtga gcagtttaat gaagctaaac agatgctatt aaggaacccc gacagctttg
      421 ggcgtatcag tatcttccct aaagaacttt ggtctagtgc tattactcca gagttaaaat
      481 ctgtttttaa taagctttgg attgaagata aatatcactc acggcttgaa aaacgtttta
      541 atttgatgtt agtcgcagcg ctgttaaccc aaaaagataa taacctgata catcaactgt
      601 ctgcgctttt tgaatttcac tatcagcaag ccccacagca attaggggta atctatcaat
      661 attattttga acaagcagga gtatgtcatt gatggacgtt cagcaactgc attatgcgcc
      721 acttctaaat cagcttcatc agcgtgcggt gaggtcggtg gtaagtcagt tggcattgcg
      781 gagcaagtcg ttgactcatt atctggaatc acaatatttt cagcctcccg gtcaggaggg
      841 gtcactgctt gcagacccgg tattcgaaag tacattcggc tggatgcctg cggatgaaac
      901 aatggaggat ttgcgggaca agctgatctc cagcccgctg gttgatgcat tgagcaaagc
      961 gccagtgcca tttcgtcatc aacttactgc gtggcgaacg attctggaag acaaaaaatc
     1021 cttgttagtg agcagcggta cgggatccgg gaaaacagaa tgctttatgg taccggttct
     1081 ggaagatctt attcgtcagc aaaaatcgac ccgccagtca ctaacaggga cacaagctat
     1141 tttcttgtat ccgttaaatg cattaattaa cagccaacag gagcggcttc gcgaatggac
     1201 gcgcggattt aaggggaaaa tacgcttcgc gctatataat ggcgaaaccc ggcataccaa
     1261 atacgaaatt caggaagatc agttgaaagt acctgaacaa gctttgagtc gggaagccat
     1321 ttatgaagca ccaccttcca tcatggtgac gaataccacc atgctggaat atatgctgat
     1381 tcgtcgtaaa gatgccccaa tcatcgaaaa gtctcagggg atgttaaagt atatcgtcct
     1441 ggatgaagcc catagttata ttggttctca ggctgctgag ttagcgctgc ttttacgtcg
     1501 tgttatgcag gctttcaatg ttggacccgg cacagataaa cctgtgcaga ttatcgcgac
     1561 ctctgccact attggtgaag actctcctga aggtaataag gagcttgcta aatttgtcgc
     1621 tgatctcgcg ggcgttacag aacgtgatgt caaagtggtt cgcggttatc gacaaatacc
     1681 gcgggtatcg gagtcgctga ttcgtcatga ataccccaca tctttaactt ctcttaaatc
     1741 cctgagccct caggatcttt accaacagct ttgtcattac cgtgtggccc agcaattgcg
     1801 tcaggcgtta actcatcccg gacggcaggc agtacgtttg agtgagttgc tcaatgttgc
     1861 cagaagaacc tggccagata ttaatcaccg tgagctactg caactcctcg atctaatggc
     1921 aagatcgcga gaaggtgaac tggcttttac gccattacgt atgcatgggt ttatcagaac
     1981 actggcgggt ctatgggcat gttcaaacaa acaatgctca cataaagcac atgaattaaa
     2041 tcagagtgac tggccatttg gtcaggtatg gtttgagcag cgccaatatt gtgactgtgg
     2101 agcgccggta tttgaagttt tacgttgctc cgggtgtggt tcagcttatt tgtctgccaa
     2161 agaggagatg cgcggtgatg gaacgaactg gttggtagcg caaccagccg ctgccgaagt
     2221 ggatgagttt gctcttgatg tagatgttta ttctgaagat gaagacaacg acgaattaga
     2281 caataactct caatttgatc gtctgattgc gagcgatggt gaaaaatata tcatttcact
     2341 ggaagaaacg acggcgggga aaattgatag tgaagcgcaa aagcactatg aaataaacct
     2401 gttaagaccg gaatctcgag gtgagggacg caataactcc tttgcttgtg tttgctgtgg
     2461 tgacactcaa cggaaaaata atcctttatt ccgtccgctt cggctcggcg cgcccttttt
     2521 tttaaatgaa attattccta ctctgctgga gttttctcca ttaccgcaaa caagagaaga
     2581 gcagttagga ccgtttaatg gtcgacgctt gctcaccttt acggattcgc gtcagggtac
     2641 cgctcgcatt tcagcccgct tacagcagga tgccgataaa gcgacgttgc gagctctttg
     2701 ttaccaggag ctggcaaata ttacagagcc ttcagcaaaa ctcagcccgg cacactgtgc
     2761 actgttagag aaagttatta gccagttttc tgtcctccct ttaaaaaatt ccatcgacgc
     2821 gctgaaaaaa gcgatggctg cgaatgagga gcttagcccg acggacattc aggcacttaa
     2881 aattattgag ccgatggtga gttccttaag cacggagcat gctgaagcga tgcaaatact
     2941 tcaggctaac tctggcaatc agcagaagaa aaccatgtca tggcaaacgc tgtctgaaca
     3001 gctggttggc agtgtggacc ttgccgatag aatgagaggc agttttaaag aattgtctgg
     3061 gcttgatctt actaaacaac agtttgcaga tttttgttta tacagcgagt ttggtcgccg
     3121 ccctaaaaat gcctggaccc tcgaaagtct gggtctggta gccattcact atccattcat
     3181 tgataaagtt acaacctgcc cgcaggactg gaagacatta ttacccgatc ccgataagca
     3241 attagcggaa tggcgtaatt tattgaagat tactcttgat ttctttatcc gtgaaaacag
     3301 tgccgttttt tacgaaaacg agcaataccc tcgctggatg ggggcacgtt tcccggtaaa
     3361 aatgctccag gggccagata aaaaaaacaa agggcagaaa cgtgatcaac tttggccaca
     3421 aatacgcgat cgccattata accgggtcat taagctatta atcgcagtct ttccggcaat
     3481 acaacctgag caggctcact ggaaatcgtt agttaaccac ttaatgattg aggtctggga
     3541 tgcaattcga ccctgcttac gtcagtttga gtcgggttat cagctggata ttaaacaaca
     3601 agctgaattt tacagtccaa agcaagtgtg gcgttgcccc tatactcgac gtgcgttaga
     3661 cgtcacgttg ctgggatact ctccgtattt gccgggtagc aaagaaattg cacctgaaaa
     3721 agcagtgttg attgaaatgc ctgagcttcc tgtacgtcac tggcgtttgt ctggtggtgg
     3781 agaaatcgcc cgagaagaac gtctggagtg gctggaaagt aatgcgctga ttcaacatgc
     3841 ccgcgaggaa ggattatggt ctactcgcag cgaccgactg gccctgaaag atagctggta
     3901 tcgtctggag gaacactctg cgcagagaac gcctgagcag aaccagttta atgaaaaaca
     3961 atttaagtca ggcaaagtta atgtgctgaa ctgttccacg accatggaga tgggggtgga
     4021 tattggtggt atgagtttgg tggcaatgaa caacgttcct ccagcgccgg caaattattt
     4081 gcaacgtgca ggtcgcgcgg ggcgtcgtgg agagagtgca tctgctgcca ttacgttatg
     4141 taaaaacacg gcgcatggaa tggaggtatt caaagacccc ctctgggcgt tcaacactac
     4201 tgccagtgcg ccacgggttc gttttgggag cagtagtatt gttcaacgac atgttaatgc
     4261 cctggtttta ggtctgtttt tacgagctga agttcctgat gcgacaaaac tgagttgcaa
     4321 atggttcttt gaaggtgatg agagccagtg tcttcgtttt ctccattggt taaatcacca
     4381 ggccgatcag ctggcggata aacttaaacg ccttactcag ggaaccgtac tgatgtcatt
     4441 gacggcaacc cagctcttga cccgcacaca agcaatgatg cagcaggttg atatccgctg
     4501 gagaagccaa cttgcgatac tgttggaaaa tatcgaagcg ttgaaggcgg ataacagcgc
     4561 gtgggaagaa acaccagcag gtaaagccat cgcttatcag cttcgtgatt atcgcggtgc
     4621 atatctgttt tctaaactca ttagtgaagc gttcctgccg gggcatggtt tcccggttgg
     4681 cgtagtcaat ttcaattatc tgacggcaga tgagctggaa aaacgtcgcg caataaaagc
     4741 cactcaggct gaccctaatg aagggggaga aagcttttct cgtcgtattg aaaaattgcc
     4801 cagccgtgat ttgcctacag cattacgcga gtatgcgcct ggtgcagatg tggtattggg
     4861 cggaaaagta tatcgttcct cgggcattat gcttggcaag gtgcttgcca gcgggcagga
     4921 gctgtcaggc gatcatcata ttccgtggtt ctggcattgc cgtaaatgtg gtgcgggggc
     4981 aaccagtact acccatcctg tcgaatgtag tcactgcaaa gcggatatac agcaacttga
     5041 tgtgaagcgt tatttacagc ctgttggttt tgcgacagat attcgttatc aggcacataa
     5101 tgatgtatcc atgcctgcac aacttccctg gaaagatcca cgtgttcttg taccgtcttc
     5161 ggtgtgggtt tctttacccg atgctgggct aggacgttac cgttttagcc atagtgggga
     5221 attgttccac ttcagtgaag gggaatttgg gcacggctac gcaatttgcc tctcatgtgg
     5281 tcgggcagaa tctcaaactc aaccccagcg tacccctgaa aatctaaaga acccagagag
     5341 ggaaaatacg cattacctgt tgcgaggggg cagtaacgat cgtcagggga gcaataaatt
     5401 gtgccacggt catgtgcata aagatctgtg gttgggatat agcagtcgta cagatatggt
     5461 tgagctgcaa ctcaatgatg ataatggcct gttgatccgt gatgaggttg ccgcgcgttc
     5521 ccttgcggtc gctttgcgtg aagggttagc gcataaattg ggtatcgaaa atactgagct
     5581 gggcgtgacg acgcagcaag cgcgcgatat caatggctat accggatatt ccatcttcat
     5641 ttacgacaat aatgcgggtg gtgcaggtta tgcagtgcaa ttgatcgacc attgggctga
     5701 tgtctttaat tacgccagaa aattgctcga ttgcagttgc gataaattct gtcaccactg
     5761 cttgctcagc tacgacagtc agcattatgt caacagatta gatcgtcacc atgcattaac
     5821 cctgcttacc aatgtacggt tgcagcgact taacctcgcg ccagaatatc agtacttcgg
     5881 cgatggtagc cgggttgaaa ctaaccctct gagtttacgt attgcgcaat gcctgaacag
     5941 cgagatttat gactcctgct cgctggttct tgcggggccg caggaacagt gggactttgc
     6001 ccaatggcca ttatttaaag agttgctcca gtttgcctcc tccggtggga atgttgagtt
     6061 gctggtggcc acgccgctgg caaacttaac tgatagctcc agacatcagc tttccgcgtt
     6121 ggcggcgatg ccgggaggtc gcttacaagt gaaatccatt gccactgcgc agctcatgca
     6181 aggaaagggg cgctggcttg cgcaggtgac tcgcgaaggg caaagccaac agtgggccgc
     6241 agacgacagc gctaccgttg cacctggtga attatgggga caaagtgcgt cttcaccagt
     6301 agtgacactt aaaggaacgt ccggaaaaac cttctcgggt cagaccttaa gtgcagaaga
     6361 tttactgccc gccttaccca ccggggcggt gcgcatcaat ctctgtgaac aactggacgg
     6421 gccgctggaa ggttttggtt cgcgtttctg gtcgttagtc acgcaacagc acgctggctg
     6481 gaagcaggca tttacccgcc acaaagagat aacgcatgtt gagtacagcg atcgttatct
     6541 taatagccca ttcacggcgc gtttgttagg tgaaatctta accgagctgg ttgaacaggg
     6601 aatggcggaa cgcgccagcc tcactgtttg cgtcaaaaaa ttggattaca actctcgcca
     6661 acacgacgct ctctataacg cgtggcttaa tgaggaagat cgtcagcagg ttgtgaccac
     6721 tttacttgaa gaaggatatc tggggcctgc gtggccggga gcaattagct ggttaacggg
     6781 ggataaccag agtacggagc atggacgaga actgacagtc accttcagcg atggcagtca
     6841 gcattatgtc ctgctcgaca tggggcttag ctactggcgc tgtattgaag ataccttctt
     6901 tgattttgcc ctgcgagtac cgcagcaagt tgaacggctg gcaaacacgc gagcgcgagc
     6961 tgttgccccg ggtaatgacc ttaggagtta tattatcgct ggataaataa ttgctcccct
     7021 ggggatatct tccaggggag ttgacccagc tcacttttta ataatttttg tttgaaaatc
     7081 atgcgcaatt tatgcgcatt ccctcccatt ttgtcacttt gccgggggcc ttcatgatat
     7141 ctttaaggcc ctctttttta tcgcagtgtg ccgcgccaca tttttcagga tgacgttacc
     7201 gccactgccc caaatagaca gaaaaaccga tgaataagca aaattatgca cctggtatgc
     7261 gggtggttat tcgtgatgcc gaatggcgta ttcgccgggc ggatgacagt ggtgatggtg
     7321 ggtatttgct gacctgcgat ggtatttcag agcttgtgcg cggtaaagaa gggttatttc
     7381 tgaccaaact ggaacaaaaa gtagagatcc tggatcccgc aaaaacgcat ttggtggaag
     7441 atgaatccgc caattatcaa gcggcacagt tgtatattga aagtcagtta cgccaacgcg
     7501 taccgacaga cagtaaagtc cattttggtc atctggcggc gatggattcc atgccgtttc
     7561 agctcgatcc tacgcggatg gcgctggcac aaccgcgtca gcggatactc attgccgacg
     7621 ctgttggtct gggtaaaacg ctggaagccg gtattctggt ttcggaactg atccgtcgtg
     7681 ggcgcggaaa acgtattctg gttctggcgg ttaaatcgat gctgacacaa ttccagaagg
     7741 agttctggag ccgttttgcg atcccattga cccgcctgga ttctgctggt ttacaaaagg
     7801 tgcgtaaccg catcccaacc aaccacaacc cgttccacta ttttgacaag accattatct
     7861 ccatcgatac gctgaaacaa gacattgaat atcgtcacca tctggaaaat gcctggtggg
     7921 atattattgt cattgatgag gcccataacg tcgcagaacg tggaaccagt tcgttacgca
     7981 gtaaactggc aaaactgctc gccgggcgca gcgatacgct gattatgctt tcagcaacac
     8041 cgcatgatgg taaggctgaa agttttgcca gcctgatgaa catgcttgac ccgacggcga
     8101 tcgccaaccc gaaagagtat gaatacgccg attttgccga taaaaatctg gttgtccgtc
     8161 gcttcaaaaa agacgtgaaa gatcagatgt ccggcgagtt ccccgagcgc aatattgtca
     8221 aattgactcg cctggcatcc ggtgccgaag aagaagcata ccgtcgtctg gttgagagcc
     8281 agttccgtga tgatgacgat gaacaggccc agtctaacaa aggtcgtctg ttcaagataa
     8341 ccctcgaaaa agcgctattt tccagcccga tggcgtgtgc cagcgtcgtg gcgaatcgtc
     8401 tgaagcgtct tgagagccgt aaagatcata atagccagag ccagatcaac gaactggagt
     8461 cattgctgtt ggcgctgaat aacattgatg ccagccagtt tagcaaatac cagttgctgc
     8521 tcgacaccat ccgaaaagac ctcgcctgga aagccaataa cacggaagat cgcctggtga
     8581 tcttcaccga aagtattaaa acgctggagt tcctcgaaca gcaactgcga gcggatctga
     8641 aattgaaaga tgaccagatc gccacgctgc gcggcgatca gggcgatacg gtattgatgg
     8701 aaaccgtaga agcctttggt aaaacgcagt cgccgttgcg tcttctggtt tgttcggatg
     8761 tggcatctga agggatcaac ctgcatcacc ttagccacaa aatgattcac tttgatattc
     8821 cgtggtcgtt gatggtattc cagcaacgta acgggcgtat tgaccgctat gggcaaaaac
     8881 atcagcccca aatccgctat ctgcttaccg aggccagcga accacagatc aatggcgata
     8941 tgcgcgtact ggaagtgttg atcaacaaag acgaacaggc gcagaagaac attggggaca
     9001 gttcggagtt taccggcaaa tttacccagg aagaggaaga agagcaggtt gcagagttca
     9061 tgatgcagga tgatggtgcc agcctgtttg atcaactgct gaacagcaac gtctcggaaa
     9121 gcgccgaaca cgatctgttt ggcgaaatat gcagtgcggt ttccagcgat gcctcaatgg
     9181 tcaccgaaac agacaccagc ttgtttgcca gcgaacaggc gtactgcgaa agagcattgg
     9241 gttacctgaa agccagtggt caaactatcc agtatgaaac cttgcctgat aataccttgt
     9301 cgctggtggc accggaggag ttacgccgcc gcttcaacca gctaccgcct gaaattgccc
     9361 cggagaactg gcagctctat ttaagtcagg ataaaaccgt catcaccgac gcgattgcgc
     9421 gcgctcgcgg tgagcaacat gcctggcccg atgtgcagta tctctggcaa atcaacccgg
     9481 tagtgcagtg gctggacgat aaaatctctt cggcttttgg tcgtcatcag gctccggtta
     9541 tccggctgcc atacctgctt gaacccgatg aagatcactt cattctttcc gggttattcc
     9601 cgaaccgtaa atcacatccg atggtgaacc cgtggatagt ggtgagcttt aaccgtgaat
     9661 cgctaatcgg cagtcagcct ttcgctgagt ttttacaacg tcatccgcag ttgagtaaca
     9721 agctgactaa cagcggcggt aaagatcgta atcaccaacg ccagcaggat ttactggaag
     9781 cggctattgc tcatgcccgg gaggtattca tccatgatcg gaatgcgttt gaaacgcaca
     9841 ttaaccagca actgaatgag catctgcaaa agctggacgt tttgcgtggg cggcagttga
     9901 gccaacttga gctggatttt gccgataaca aacagcaatt gtcagtcaag cagagccgta
     9961 aagagcagag acaacgcgaa atcgaacaca attttgacag ctatatcgaa tggattgagg
    10021 acaccatgac gactgaaaaa gaaccctaca ttcaggtaat tgctgttatc accggagcgg
    10081 agggttaatc atggcgctgg tcggtattaa taacgaaaac gaattttact ctaaccacta
    10141 tttgggtgag gttttcacca gtgatatccg cgatgtgctg gaaccctgga tagcccagga
    10201 aaatgcagcg cgtgaagcgg agcgtgccgc tcgtgaacag ggcaaagacg tggagccggg
    10261 ataccgcgct ccgtggaacc agtttaacag tctggcgact gagtttttcc gcaaacttgc
    10321 cgagcacgaa aaacagcgtc agatcccgca gcgtctggcc gatcaacgta atcgctggca
    10381 gccattgtta aaggcgctgg gctacgaaat tacgccacag atccaaatgc tggatgacga
    10441 tacaccgctg ccggtactgg cgcgttacaa cagcactgac ggtagcccgt ggctgtggat
    10501 tgttgaagca cacgatcagg aagaaggaac gctggatccg ctggcgctct ccttactgac
    10561 cgcgcaattc ccggcggata ccgacaaaca taagcacgac agcctgcgca aaaaagccaa
    10621 cggtgaatat cgcagctggc aggatttgct ctctacggcg gtcttcaccc aaaatgaacc
    10681 gccgcgtttt gtgctgctgc tcggtaaccg tcagctattg ttgttggacc gtactaagtg
    10741 ggcgcaaaac cgtctgctac gttttgattt tgaagagatt ttaagccgtc gtgaaacgga
    10801 tacgctgaaa gcgactgcgg tgttgttaca taaagattct ctgctgccgg gcagtggggc
    10861 accttatctt gactcgctgg atgacaattc gcacaaacat gcgtttggtg tttcggaaga
    10921 tctgaaatat gccctgcgcg aaagcattga gttgctgggc aacgaagcga tgcattatct
    10981 gatcgaccgt ggcctggcaa actatacagg taatcgtgcg gtggacccgg atgaactgag
    11041 ccgcgaatgt ctgcgttaca tgtaccgcct gctgttcctg ttctacattg aagcgcgccc
    11101 ggagctgggt tatgcgccaa tgaccgccaa aacctatctg caaggttaca gcctggaaac
    11161 gttgcgcgat ctggagatga tcccgctgac cagcgaagaa gatcgcaacg ggcgctactt
    11221 ccacgacagc ctgaatatgc tgtttaaact ggtgcgcgaa ggctacaacg gcggcgtgaa
    11281 aatgcagagt gacctggaga gcggcgaccg gatcaccatc catagtcatc agttcagcgt
    11341 cccgcgtctg gaaagtcatc tgtttgatgc caacaacacg cgcattctta accgcgtggt
    11401 attccgtaac gaaaccctgc aacagattat ccaggcgatg tcgttaagcc gcccggccaa
    11461 agggcgcttt aaccgccgcg gacgtatttc ttatcgccag ttgggtatca accagttggg
    11521 tgcggtgtat gaggcgctgc tctcctatcg cggattcttc gccagcgaag atctctacga
    11581 ggtgaagaaa gccggggaag agtttaacga gctggagacg ggttacttcg tcagtaagga
    11641 tgagattagc aaataccacg aagacgagaa ggtctacgag aaagacggca gtctgcgcat
    11701 tcaccgcaaa ggcagcttta tctaccgtat ggccgggcgc gaccgtgaga aatctgcctc
    11761 ttattacacc ccggaagtgc tgacccgctc actggttaaa tatgccctga aagaactgtt
    11821 taaggagcag attgatccca ttagcgatcc gcacgccaaa gctgatgcca tcttaaacct
    11881 caccgtgtgc gaaccggcga tgggcagcgc ggcgttcctt aacgaagcca tcaaccagct
    11941 ggcggaagcg tatctgttcc acaagcagca ggcggaaggt cgccgtattc cgcaggatcg
    12001 ttacacccag gagttacagc gggtgaaaat gtacattgcc gacaacaacg ttttcggcgt
    12061 ggacttaaac ccggtggcgg tggaactggc ggaagtgtcg ctgtggctga acgccattag
    12121 tggcgatgcg tttgtaccgt ggtttggtta ccagctgcac tgcggtaact cgctggtggg
    12181 cgcgcgccgt caggtgttca acaagagcga actgacctac aaaaaagcca aagatccgag
    12241 ctggcttaac agcgagccgg tcgaactggc gatgaacacg ccgcgtgaag agacgcagat
    12301 tttccacttc ctgctgcccg acggcggtat ggctaactac agcgataaaa ctgttaagca
    12361 gcgttatccg gatgacttca aagcgctgga cagctggcgc aaagagttta ttaaaagctt
    12421 tgccgggcat gagattgctg atgtgcagcg tatcagcgaa aaggtggaag cactgtggaa
    12481 cacctatcgc cagcaactta aagcagaacg tctgaaaacc gccgacagct acccggtgtg
    12541 gccggcagaa aacagcgagc agacgcgttc ttcgctgagc agtaaagatg aaacctttag
    12601 cggtcgtctt gaagataaca gcgcctacca gaagctgcgt tgggtaatgg actactggtg
    12661 cgcgctgtgg ttctggccga tcgacaaagc cgatgagcta ccggatcgcg gcacctggtt
    12721 gtttgagatt gaaaccctgc ttgacgggat tgtaatcacg gaaaaagtca ctgaagttgc
    12781 ggagcacacc accggcgatc tgtttgccga agaaggcctg ctgcgagaag agtcttcgct
    12841 gttttctgtt gctggtcgtc tgaaaaccga ggtgttgttc cgtcatttgc cgcgtctggc
    12901 gattgtcgat gccctgagaa agcagcaccg tttcttccac tgggatctgg agttctgcga
    12961 cctgtttgcc gagcgcggcg gttttgacct gatgctcgga aacccgccgt ggctgaaagt
    13021 ggaatggcag gaagcgggcg tgctgggtga ttacgagccg gaatttgtgc tgcgtaagct
    13081 gagcgcctcg aagctggcaa cgttgcgtat tgataccttt aaccagatcc cggcgctgga
    13141 agcggcctgg cgcagcgagt atgaaggctg tgaagggatg caaaacttcc tgaatgcgca
    13201 gcagaactac ccggtactgc gcggggtgca gaccaacttg tataaatgct ttctgccgca
    13261 ggcctggcga ttaggggcgc agaaaggcgt ggcaggtttc ctgcacccgg aagggattta
    13321 tgatgacccg aaaggcgggc aattacgtgc ggcggtatat ccgaggctga gggcgcattt
    13381 tcagtttcag aatgagttaa atttgtttgt tgaagttgat caccatgcga agtttagcag
    13441 caatatttat tctgctagcc ctagcacagt gggatttgaa catatatcta atttgtatgc
    13501 tccgcaaact attgatgcat gttttgaaca ttctggcagt ggggacattc ccggtctcaa
    13561 agacgagatt gagagcgagg gaaaattaaa agttgtatgg aacacatctg gccaccgttc
    13621 tcgattaata agtatcgcca ctcatgagct agaattattt gctcgtctat atgacagcga
    13681 aggaacgcca gcctggcagg cacgtttgcc agccttacat gctaaacaac ttgttgctgt
    13741 actggaaaag tttgctaatc agccgaatag attaggtgat ttgcaggggc agtatttttc
    13801 aacggttatg ttcgatgaaa catatgctca gagggatggg acaattttac ggcagactca
    13861 attccctcaa gattcatcac aatgggtact gtctggccct catttctttg ttgggacgcc
    13921 gttctacaag actccgcgcg aaaactgtac gcttaacagc gattatgact gcctggactt
    13981 gctaactctg cctgacgact atctgccgcg cactaactac attccggcat gtgatgcaca
    14041 ggagtatgca aaacgtactc catgcgttac atggactgaa ctggctgaag atgaaccgaa
    14101 gaaggtaaca gattattatc gcttagctat cagagccatg ttggctcaat cgggggaacg
    14161 tacactaatt agtgctattt atccgccaga aataagtcac atgaacgcag tacgttctta
    14221 ctgctatagc tcacagaatc tgttactcga acattcaggt atgtgttttt ctttaccttt
    14281 tgattttatt tgtaaatcta ctggcaaggc aaacttacat cagatgcttg atggtttctc
    14341 atacgtatta ttcaatccga gacaaaaggc attattatac tgcttagtat tatcattaaa
    14401 ttctgtaaat gatgtatatg ctggcctttg gcaatcctgc tacaccccag acttcaacac
    14461 ccagcgttgg agccgcgatc tcccgcagct cccccaggat ttcttcgcca aactgacccc
    14521 agagtggcag cgtaactgcg ctttacgctc tgactacagt cgtcgtcagg cgctggtgga
    14581 aatcgacgta ttggtggcgc aggcgctggg gttaactctc gaagagctgc ttaccattta
    14641 tcgcgttcag ttcccggtga tgcgccagta cgaagcggat acctggtacg atcaaaacgg
    14701 tcgcattatc tttaccccaa gcaaagggct ggtgggcgtt ggcttgcctc gcaccgcgcg
    14761 taaagctgac ctgaaaaacg gctttgtctt taacgtcgac agcccggagt ggaccggcgg
    14821 tgactgcacc gatcaagcta tcggttggga tgatgtcaaa catcttaaaa ccggtaccgt
    14881 cagcgtcacc tttgatgatt atacccgcag cgacgaaggt gagcgccgta ccgtcacctg
    14941 gcaggctccg tttatcaagc cagatcgcga agatgactac aaagtggcct gggcgttctt
    15001 tgcacaagat aaggagagcg cctgatgttg ccgtccgttg tcagtcggca ggtcgccgac
    15061 agcgttgccg cctttttacg ggcggcgttc ccgctaaaca gcccgctgtt taacggtgag
    15121 aataacgata atgtctcaat gctggagcaa tttctggccc agccggaaac gctgctgaaa
    15181 ggaccatacc tttccgccca gttgccgttt cgtaaaagtg acttaccgct gaattttttc
    15241 cctaatctga cgctgccgtt cccgccgcac gcgcatcagg cccgggcatt tcagcgtctg
    15301 ggaagcgacg cgccacagcc gacgctggtc gcaaccggta ccggctccgg taaaacagaa
    15361 tgttttatgt tcccgctgct caaccactgc gcgggcgcgt cacaagccgg ggttaaagcg
    15421 attatcatct acccgatgaa cgccctggca accgatcagg ccagccgttt cgccaaaacc
    15481 atcgccagcg atccacaact gcatggcaag gtgaccgtcg gcctgtttgt cggcgacagc
    15541 gaaatcgaac ccagcaaaaa aatgtcggca gaaaaggtca ttacctgtaa gcacacgctg
    15601 cgcgaaagcc cgccggacat cctgttgacc aactacaaaa tgctcgacta cctgctgatg
    15661 cgccctggcg atcagccgct ctggcgttac aaccagcccg gttcattgcg ttacctggtc
    15721 gtggatgaat tgcatacgtt tgatggcgcg caagggtcgg atctggcctg tctggtgcga
    15781 cgtttgaagc atcacatagg tgtggataac caacgattcg cctgcgtcgg gacatcagct
    15841 accgtcggcg acgaactggg gcaactgctc gattacgcaa aaacaatctt tgaccagccg
    15901 tttaccgatg acgccgttat tcgcgaagat cgctacacgg cagcggagta cttgcagaat
    15961 tacgccattg cgtacagtca gtatccgggg ccggaggtcg cttcggccct ggacccgcag
    16021 agttacgcta ccccggtagc gtatcttaac gggcaaatcc ctttgtggtt ccccgacagc
    16081 gacttgcaac tcccggacga tctggagagc gacgacgggc gtgaaaagcg tattgcgctg
    16141 gggtcactgt tacgccgcca cagcgtgatg catgtcttac tccatgatct gcaaggcggg
    16201 atcctctctg aacgagagtg tctggaaaat ctccaggtca tgctggcaga aagcgccgac
    16261 catgcccgac gcgtgctgca aagcattctg gcactgatcg cgcaggcacg tctcgaagta
    16321 ccggaaacgg aacacgatcg gcaaaaacgt ctccaggcga agaaagcgcg tccggtgctg
    16381 ccgtttgtgc aactgcgctc ccagttgtgg cttcgtgaga tgcgtcgtct ggtcgccagc
    16441 gtggcgaaaa cgccccgact ggtgtttgcc gatgatgtgg cggtggaaga tcgcgaacac
    16501 tatttgccgg ttatccactg ccgtgactgc cacgcaactg gctgggccag cctgcgtcat
    16561 gggcaaagcg ttcagttgga aacagacctc gacggcattt atcgccagtt ctttgaaaaa
    16621 gggcgctcca tcgtccttgc tttcccggat aacaacgaaa aagcggtcag tggcgcacac
    16681 cagaaactgt gtcctacctg tctgaaactg aaccgacagt ccagcgtcca gtgtggacat
    16741 tgcggtcata cggaactgtt gcaggtattg atcccggata tgctcaaaga gcgtaaggac
    16801 gagctggagt ttgctaatga atgcccgtac tgcaacagca aacaggggat ttcgattctg
    16861 gggtcacggg ctgccagtct gtcggcggtg atgattcacc agctttatgg cagccagttc
    16921 aacgaacata aaaagctgat tacgttttct gactcggtac aagacgccgc gcatcgtgcc
    16981 agcttcttta ctgcccgtac ctggccgctg atgattcgtg ggcaaatcgc cagtgttctt
    17041 agcgaaaaag agacattacc gctggatgcg ttcgcggcgc gtgtttgcca acaaacgcgt
    17101 gaacgttcac cggacgatgc tcattttgca gcaaacgtca tcgcaccaaa tatgcagtgg
    17161 ctgaatgatt atcagacgct gaaaaatgaa ggcaaactgg cggagaactc cgatctgccg
    17221 gagctggttt ccctgcgcct cgactgggag gtgtacagcg aatttaccct gcgcgcgcgt
    17281 atcggacgta cgctggaagc caccggttat gccgttgccg gagtggataa agatcgcttc
    17341 aatccggcaa ttaccgccct gcatcaacag ctttgtgaag agctgggtga tgagatgtct
    17401 ggtgtgagcg tcgagcaggt agcgcacctg gcgctggggg ttatctggta tcaacgccag
    17461 gccggggccg ttatgcatcc ggcgttcgaa agttatcgcc gtgatggcac gacctttatg
    17521 atgacgcaaa aagagcgtac ctcgcgctat atgccttcta tcggtccgaa aacgcgtaaa
    17581 ccggcttatg cgtcgtttga aaaaattaat ggtttccatc gcctgattgg cgcgaagtct
    17641 aaccccagtt ggtatcagca ttggatcaat cgcacgctga gtaacggcaa taatctgttt
    17701 atcagtagcg ttgcggaaac agtgttacga cgcctgttta cggcgttgaa aatagccgga
    17761 gtagtaaaag attttgacac caaagggcgt gaagcctggg gactggtgcc ttcggcgctg
    17821 gtggtgagcc gtgacgtcgt tcagcttaat tgctcatgtt gccgtgaggt tgtccgggct
    17881 ccggccgatc aaggctggca gtggcgtaat gcgccttgtc tctctttacg ttgtgaaggt
    17941 cgttaccagg aagtcgacaa tacggcgacc tggcgttggg agaacatgga catcgcgcgc
    18001 gtacagggcg cagaacacac ggggctgctt agccgtgaag atcgcgaagt caccgaaaaa
    18061 tcgttctacc gcggcaacca accgtggaat atcaacctgt tgtcggcaac accgacgctg
    18121 gagatgggta ttgacgttgg ggatctttcc accgttttac tctgttctgt cccacctgct
    18181 caggccaact acttgcagcg tattggtcgc gccggtcgta aagatggtaa cgcgttaaac
    18241 attaccgtgg cagaagggaa cccgcacgat cagtttttct ttgaagaacc actggagatg
    18301 atgcagggcc aggtgcaggc tcctggcgtc ttccttaatg caacggctat tctcgaacgc
    18361 cagctggcag ctttttgtat ggataactgg gtgaaaaccg gtgtaccaga atccgctatc
    18421 tgtaaaaacg tcaaacagat gctggatgag ctggagttcg ggcgtaaatc gggttttccg
    18481 tataacttac tgcgatatat cgaacagcat catgcggaga ttgctgcgca atttacggca
    18541 atcttccccg atcttgccgc agaaactcgc caacagctgt tgtcttatct tcagggcgcg
    18601 ccaggacaac gctcgctggt gcagcgtatc gaagaagcgt taaaactgct ggtggaagat
    18661 cgcaaatctt tgcgctcacg catcgataaa ctcaagcgca gtatcgataa actggagaac
    18721 gcgccgcgcg atcaaaactt tgatagcgat atgcgcgagt taacttcgga acggcaggcg
    18781 ctgatggcgc tggtcaatca aatcaacaat aagcaaacgc tgaatttcct cacggatgaa
    18841 ggtctgctgc ctaactacgc cttcccggaa gccggtatta cgttacgttc tgtgctctgg
    18901 cggcgtaaag aaggcgggga agcacgtgaa tatcaaaaca ccactttcga atatgaacgc
    18961 ccggcatcaa cggcgctggc tgagctggct ccgctgaata atttctatgc cggtgggcat
    19021 aaagttgaaa ttgagcagat agacctgaag gtctctgagc cggagaactg gcgtatctgt
    19081 agccactgta actacagcga aaatatcgac cagacgggcg accagcataa atattgcccg
    19141 aaatgtggca caccggggtg ggccgatgcc ggacaaaaaa ccacgctgct gaaacttcgc
    19201 caggtttatg cccgcgccag cgcacgtgat agccagatca gcgacgagag cgacagccgt
    19261 gaacctgctt tcttccagcg tcagctattg gttagtttcg aaaaagaaga tgtatcggcg
    19321 gcatacgcga ttgaggaagg cgaagttccg tttggttttg aatttttaag caaagtcacc
    19381 ctgcgggata tcaacttcgg caaaatggcg gatgatgcca atgagttgat gattgccgga
    19441 gaagcgaaga agcgaaccgg gtttaaagtt tgcctcgggt gcggcatggt gcagcgtcct
    19501 cgcgatcatg aaccacgtca tgatttgagc tgcaaatatc gcacggaacc ggaaaaggcg
    19561 aagttcgaag attatctcta cctctatcgc cagctggagt cagaagcgtt gcgtattctg
    19621 ctcccggtaa gcagttacag taatgaccgt gtcgtggagg cttctcttgg ggcggcgatc
    19681 cagctcggtc ttaaacatta cttcaagggg aatgtcgatc acctgaaagg cgttgtgtac
    19741 cgcgagccgg aaaatgaagg cgaatcctgg cggcagtatc tggtgattta cgataccgtc
    19801 ccggggggga ctggttctct taaagaactg atgcgcactc ccgacaattt gctgaaatta
    19861 cttgaactgg cctatcaggc aatagtggaa tgcagttgca acaatgatac gcataaagat
    19921 ggttgttatc gttgcgttta cgcttaccgt gaccgtgggc gcatgaagta tgtctcccgc
    19981 gatcaggcgc gtctcttgct ggcgaaaatt ttgcaggcaa gagattcgat acgggtaatt
    20041 gattccatca aaaacatttc cctcgaagcc atgatgggca gtgagctgga aaaacggttt
    20101 attagctgcc tgcaagaaaa taaaaacctg attgtcagtc gcagttacgc gcatcagggc
    20161 gctgggtgga ttattaacac ccgcagcgaa ccgatgatga gctggcatct taaagctcag
    20221 gtggatttag gcgctaaaga ggcggtgggg atcccttgtc gcccggatta cgtgctatat
    20281 ccgcttatgc agtctgatgc cattaaaccg gtggctattt tccttgatgg ctttgcgttc
    20341 cacaaaaata gcgtcgcaga ggatgtgcaa aaacgccagg cgattcggga tagcggtaat
    20401 ttctgggtgt ggacagtcac gtgggccgat ctcaaagagc cagggcttaa acatgttcag
    20461 gatgtcctgg gacttgggca taacccggat atgaaacagc ctaagtttta taacctgttt
    20521 catgatacca actttgcggc ccttgaagcg tcgttccggg aacgcaacag ttttgcactg
    20581 ctgctggatt atctcgccga tccgggcgga agaacgcagt tatggcagag aatggctgct
    20641 gcacatgcct gggtgtggtt ggatgtgaag aagtcacagg acgcgggaac gaaacaaaag
    20701 tatgcctatg aaatgcagga aaacgcgcca gcttggcggc tagcagagct tttgccagac
    20761 gaaccgtttg tctttggtgg tttactcgat agctgtaact catcgcagca atttattgag
    20821 ctggcttcgg tgttaccaca acaagccatt aaaccgacga ccagcgtcgc acaaatgcgc
    20881 agctggctgc gtttgcatat ctgttttgac gatcgctata cgcaggatga cggttatgaa
    20941 gcgggactca atggcttctg gcgactggta aacctgttac agttcctgcc ggatatgacc
    21001 tttaccagcc gtaaagccgt gcatttaccg caggaaagcg ttgacactaa agtttcggca
    21061 acgccagagg cagtaccaga gactgatgag agttgggctg agattattga gtttggctta
    21121 ttaagcgctg aagagatcgc gttgcttcag tctcattctc tctctgcgcc gactctcggc
    21181 tacgaattgc aaaatgatga aggtgaaatt attgcggaag ccgatctcgc ctggccatca
    21241 caaaaatggg cattgattat tgataatcaa gaatttattc cattattcac aaccaggggt
    21301 tggcacgttg cattcgggcc aattgacgaa aatacattgc aacatctgtc cggaggtgac
    21361 aaatgacggt gcaaacgccc aaggtggcac tttctgatgg ttttctgggc gcattcgcgc
    21421 ggatcccaaa ggcacagcaa aaaaaggtgc aggaattcat ctccaaattc cgtcaggacc
    21481 cgaccagtaa tggacttaac tatgaaaaga tccacgatgc gcgcagtaaa aatgtccatt
    21541 cggtgcgaat cgatcaaact tatcgcggca ttgttctgaa acctgaacag ggtgccctgt
    21601 atatgctgat gtgggtggat aaacacgacg aagcatatga ctgggcgcgg cggcatgatt
    21661 gttcaattca cccggttacc ggcgcgattc aggtcattga tattagctat atcaaaccgg
    21721 cagcggaaac agtggttgat aaaccgaaac tcttcgctgc ttacagcgcg gaacagattc
    21781 tggctctggg tgtgccgcct gtctttattg atcaggtgat ggcgcttacc gatgaagccg
    21841 ggcttaatca acttgaaagc ataatgccag cagaagcctg ggaacctctg cactggctgg
    21901 cagaagggct ggattaccag gaagtgctgg aagagtttaa cgatcatcgt gatgagcctg
    21961 ttgataccaa cgactttatg gaggcgattg aacgaagtaa acgtcgcttc catgtcgttg
    22021 aaaatgaaca ggagctgttg cagatgctta atgcgccttt agaaaaatgg cgtgtatttc
    22081 tgcatccgag ccagcgaaaa ttggtggagt cgcccgctaa tggtccggta cgagtactgg
    22141 gcggtgcagg tacagggaaa acggttgttg cgatgcaccg tgcgcggtgg ttatctcagc
    22201 gtctggcaga taaacccggt aagaaagtac ttttcacgac ctttacgcgt aacctggcgg
    22261 cagatattcg cgctaatctc cagcggttgt gtacgcgaga agagatggcg cgtattgacg
    22321 tcgttaacat tgatgcctgg atgagcgacc aactaaaacg ccacggttat gatttccgcg
    22381 tggtatacga cagcgatgaa gggcgacgca aatgctggaa ctatgccttg cagcaggcac
    22441 ctgccgaact ggggctacct gataatttct atgctgaaga gtggcaacgc gttgtccagc
    22501 caaatgcggt ttacacccgg gaggaatacc tcaaagtcag ccgggttgga cgtggtacag
    22561 cgttatcccg tattcaacgg gcgaaaatct ggccagtctt tgaagaattc agagcacaaa
    22621 tggcgcgtgc caaactgcgc gaaatgggcg atgccatgca tgaggctatt gttctgttta
    22681 aagaaaagca ggtccaacta ccttatagca gtattgtggt cgatgaagct caagatatcg
    22741 gtgccccggc atttactctt attcgcagcc tggttcccga gtcgcccaac gatctgttta
    22801 ttgtcgggga tgggcatcag cgtatttatc gcaataaggt cgttcttggt cagtgtggta
    22861 ttaacgtccg tggtcgtcgc agtaaaaaat tgaagattaa ctatcggacc acagaagaga
    22921 cgcgtcaatt tgctgttggg ctgttaactg gtgtgaaggt tgataacctc gatggcgaag
    22981 cggataccag taatgattat ttgtcattac tgcacgggga aaagcccatg attacgcatg
    23041 cggcagattt caaagaagag gctgcaacgc tcgtcaagca aattcaggca ttgctggcga
    23101 atcaggttcg ttctcaggat atctgcatta ctgcgcgtac taaacatgct tgtgaccgct
    23161 acgcatcagc actgaatgac gccggaattg agacatttaa tttaggcaat gatagcggtg
    23221 acagcgatgc acgccctggc gtgcgggttg caacgatgca ccgtattaaa gggcttgagt
    23281 tccagtatgt gttcctggtg gggattaacg aaggcgttgt gccggaaata aaagcattag
    23341 catccgatga tcccgttgaa cagcgtgatg cattatttaa tgagcgcgcg ttactgcatg
    23401 tggctgcaac ccgtgccgta aaagggctgt ttgtttcatc atctggtgtc cctagtccgc
    23461 tattagtggc tgactgataa tatgtccaaa acaggccagg agccgaaacg ctcctggtct
    23521 atataaccgt tctggcaact ggcgatattt atattgctta gctcccaaca gccagacaat
    23581 atcaacttag aaattatatt cgcttatgcg gtttgcatat tgcacagtac aacctgtttt
    23641 ctctatatct aacgtaacgt caggatgtgg atgatttttg gatggggtga cacactggac
    23701 aaatatagct ggcacgaaaa ggctggcatt tccagctgac caatagtctc tggaa
//
LOCUS       DAZKFZ010000060        23153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000060 DAZKFZ010000000
VERSION     DAZKFZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1003
                     /locus_tag="ACHOQW_002115"
     CDS             <1..1003
                     /locus_tag="ACHOQW_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9573195.1"
                     /translation="VSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALI
                     PFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRT
                     RLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIF
                     QELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWG
                     HRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVEL
                     LTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1184..2674
                     /gene="dgcJ"
                     /locus_tag="ACHOQW_002116"
     CDS             1184..2674
                     /gene="dgcJ"
                     /locus_tag="ACHOQW_002116"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP9573196.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2717..3220
                     /gene="yeaK"
                     /locus_tag="ACHOQW_002117"
     CDS             2717..3220
                     /gene="yeaK"
                     /locus_tag="ACHOQW_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP9573197.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3221..3325)
                     /gene="yoaI"
                     /locus_tag="ACHOQW_002118"
     CDS             complement(3221..3325)
                     /gene="yoaI"
                     /locus_tag="ACHOQW_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP9573198.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3495..3941
                     /gene="yeaL"
                     /locus_tag="ACHOQW_002119"
     CDS             3495..3941
                     /gene="yeaL"
                     /locus_tag="ACHOQW_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9573199.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(3898..4719)
                     /gene="nimR"
                     /locus_tag="ACHOQW_002120"
     CDS             complement(3898..4719)
                     /gene="nimR"
                     /locus_tag="ACHOQW_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP9573200.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            4816..5997
                     /gene="nimT"
                     /locus_tag="ACHOQW_002121"
     CDS             4816..5997
                     /gene="nimT"
                     /locus_tag="ACHOQW_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP9573201.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6052..6399
                     /gene="yeaO"
                     /locus_tag="ACHOQW_002122"
     CDS             6052..6399
                     /gene="yeaO"
                     /locus_tag="ACHOQW_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9573202.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6421..6675)
                     /gene="yoaF"
                     /locus_tag="ACHOQW_002123"
     CDS             complement(6421..6675)
                     /gene="yoaF"
                     /locus_tag="ACHOQW_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP9573203.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            6858..7883
                     /gene="dgcP"
                     /locus_tag="ACHOQW_002124"
     CDS             6858..7883
                     /gene="dgcP"
                     /locus_tag="ACHOQW_002124"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP9573204.1"
                     /translation="MSDQIIARISQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNQKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            7916..8014
                     /gene="yoaK"
                     /locus_tag="ACHOQW_002125"
     CDS             7916..8014
                     /gene="yoaK"
                     /locus_tag="ACHOQW_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP9573205.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8017..8091
                     /gene="yoaJ"
                     /locus_tag="ACHOQW_002126"
     CDS             8017..8091
                     /gene="yoaJ"
                     /locus_tag="ACHOQW_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP9573206.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8150..8398)
                     /gene="yeaQ"
                     /locus_tag="ACHOQW_002127"
     CDS             complement(8150..8398)
                     /gene="yeaQ"
                     /locus_tag="ACHOQW_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9573207.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8545..8727)
                     /gene="yoaG"
                     /locus_tag="ACHOQW_002128"
     CDS             complement(8545..8727)
                     /gene="yoaG"
                     /locus_tag="ACHOQW_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP9573208.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8731..9090)
                     /gene="yeaR"
                     /locus_tag="ACHOQW_002129"
     CDS             complement(8731..9090)
                     /gene="yeaR"
                     /locus_tag="ACHOQW_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP9573209.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9263..9901)
                     /gene="leuE"
                     /locus_tag="ACHOQW_002130"
     CDS             complement(9263..9901)
                     /gene="leuE"
                     /locus_tag="ACHOQW_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP9573210.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10028..10951)
                     /gene="dmlR"
                     /locus_tag="ACHOQW_002131"
     CDS             complement(10028..10951)
                     /gene="dmlR"
                     /locus_tag="ACHOQW_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP9573211.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11054..12139
                     /gene="dmlA"
                     /locus_tag="ACHOQW_002132"
     CDS             11054..12139
                     /gene="dmlA"
                     /locus_tag="ACHOQW_002132"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP9573212.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12390..14000
                     /gene="yeaV"
                     /locus_tag="ACHOQW_002133"
     CDS             12390..14000
                     /gene="yeaV"
                     /locus_tag="ACHOQW_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP9573213.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14032..15156
                     /gene="yeaW"
                     /locus_tag="ACHOQW_002134"
     CDS             14032..15156
                     /gene="yeaW"
                     /locus_tag="ACHOQW_002134"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP9573214.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYSFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15212..16177
                     /gene="yeaX"
                     /locus_tag="ACHOQW_002135"
     CDS             15212..16177
                     /gene="yeaX"
                     /locus_tag="ACHOQW_002135"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP9573215.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16231..17346)
                     /gene="rnd"
                     /locus_tag="ACHOQW_002136"
     CDS             complement(16231..17346)
                     /gene="rnd"
                     /locus_tag="ACHOQW_002136"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP9573216.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17428..19113)
                     /gene="fadD"
                     /locus_tag="ACHOQW_002137"
     CDS             complement(17428..19113)
                     /gene="fadD"
                     /locus_tag="ACHOQW_002137"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP9573217.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19318..19899)
                     /gene="yeaY"
                     /locus_tag="ACHOQW_002138"
     CDS             complement(19318..19899)
                     /gene="yeaY"
                     /locus_tag="ACHOQW_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP9573218.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(19939..20634)
                     /gene="tsaB"
                     /locus_tag="ACHOQW_002139"
     CDS             complement(19939..20634)
                     /gene="tsaB"
                     /locus_tag="ACHOQW_002139"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9573219.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20692..22602)
                     /gene="yoaA"
                     /locus_tag="ACHOQW_002140"
     CDS             complement(20692..22602)
                     /gene="yoaA"
                     /locus_tag="ACHOQW_002140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9573220.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22734..23078
                     /gene="yoaB"
                     /locus_tag="ACHOQW_002141"
     CDS             22734..23078
                     /gene="yoaB"
                     /locus_tag="ACHOQW_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9573221.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5659 a         5914 c         5646 g         5934 t
ORIGIN      
        1 tgtttctatt ttctatattt tccgccaact cagttttatt tgtttaacct cgctggcgct
       61 cttttgttat ggaaaagaca acatccttga caacaataag aaaaaaactg gcattctgtt
      121 gctggcgctg atcccttttt tagtttttcc ccttctggca cacaatctga gcagttataa
      181 cgctgactat tctttgtatg tcgtcgatta ctgtccggac aatcatactg cgacctgggg
      241 aatcaactat acaaaaatat tggtttgtct gtgggcattt ttactgttct ttattatcat
      301 gcgtacacga ttagccagcg aactatggcc gttaatagca ttattatgtc tggcatcgct
      361 atgctgcaac ttacttctac tgactctgga tgagtataat tataccatct ggtatatcag
      421 tcgcgggatt gaagtctcca gtaaactgtt tgttgtgtct tttctgattt ataacatttt
      481 tcaggagctg caactctcca gcaaactggc agttcatgat gtgctgacca atatttataa
      541 tcggcgctac tttttcaaca gcgtagagtc attattgtcg cgacctgttg ttaaggactt
      601 ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc aatgcccaat ggggacatcg
      661 tgtgggtgat aaagtgctgg tttcaattgt cgatattatc cagcaaagca tccgccccga
      721 tgatatttta gcgcgactgg agggtgaggt gtttggcttg ctatttaccg aactcaatag
      781 tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat gtcgaactcc tgaccggctt
      841 tagtaacaga tatgatgttc ctgaacaaat gaccatcagt attggcacag ttttttcaac
      901 gggtgacacg cgtaatatct cgcttgtcat gacggaagca gataaagcct tacgcgaagc
      961 gaaaagcgag gggggcaaca aagtgattat tcatcatatt taagtgcaaa aatattcaga
     1021 gccatgcttt ttgcgtggct tttgcataca atttattact attaccccta aatttcccct
     1081 ccccagcggc gcagagatga gtataattag cgcccctgtg ccaggccgca atcgaacttt
     1141 atctggtttt ctcgtttcac taaccgaagg agtgccattt atcatgaaat tgcaccatag
     1201 aatgctccgg cattttatcg ccgcaagtgt cattgtgctg acatcttcct tccttatttt
     1261 tgaacttgtc gccagcgaca gagcaatgag tgcctatctg cgctatatcg tgcagaaagc
     1321 agactcctcc tttctttatg ataagtatca gaatcagagt attgccgcgc atgtgatgcg
     1381 cgctctcgct gctgagcagt cggaagtgtc gccagaacag cggcgcgcca tctgcgaggc
     1441 ttttgagtct gccaataata cccatggctt aaacctgact gcccataaat acccgggctt
     1501 acgcggcaca ttacaaaccg catccactga ctgcgacaca attgtggaag ctgcagcact
     1561 attacccgct tttgatcagg cagtggaagg caaccgccac caggatgatt acggttcagg
     1621 tcttgggatg gccgaagaga aatttcacta ttatctcgat ctcaatgacc gctatgtcta
     1681 tttttatgag ccggttaatg ttgaatactt tgcgatgaat aactggtcca tcctgcagtc
     1741 aggaagtatt ggcatcgatc gcaaagatat tgaaaaggta tttaccgggc gtaccgtatt
     1801 gtcgagcatt taccaggatc agcgtactaa acagaacgtg atgagtttgc tgacgccggt
     1861 gtatgttgca gggcagctaa aagggattgt gctgctggat attaacaaaa acaatctgcg
     1921 gaatatcttt tacactcatg accgccctct cctctggcgt tttctcaatg tcacgctaac
     1981 cgatacagat tcggggcgcg acattatcat caaccagagc gaagataatc tgttccagta
     2041 tgtcagttac gtccatgact taccgggcgg cattcgtgtc tcgttatcca ttgatattct
     2101 ttactttatc acgtcttcgt ggaaaagcgt tctgttctgg attttgacgg cgttaatttt
     2161 gctgaatatg gtgcggatgc acttccgctt ataccaaaat gtgtcgcgag aaaatattag
     2221 tgatgcgatg actggactgt ataatcgcaa aattttaacc cctgaactgg agcagcggtt
     2281 gcagaaactg gtgcaatccg gttcttcggt gatgtttatt gcaattgaca tggacaagtt
     2341 aaagctaata aatgacaccc tcggtcatca ggaaggggat ttagcgatta cgttgttagc
     2401 tcaggcgatt aaacaatcga ttcgtaaaag tgattatgcc atccgactcg gtggcgatga
     2461 attctgcatc attcttgtcg attcgacgcc gcaaattgca gcacaactgc ctgaacgtat
     2521 cgaaaaacgt ctgcaacata tcgcgccgca gaaagagatc ggcttctctt ccggtattta
     2581 cgcgatgaaa gaaaacgata cgttacatga tgcgtataaa gcttccgatg agcgtttata
     2641 tgtcaataag cagaacaaaa acagccgttc atgataacct tctgtggttg tttgcttgta
     2701 atctcaggag cgtgaaatga ctgaaatggc taaaggaagc gtgacgcatc agcggttaat
     2761 cgctttatta tcacaagaag gtgctgattt ccgcgtcgtt acccatgaag cggtaggtaa
     2821 atgtgaagcg gtgtcagaaa ttcgtggcac cgctctaggc cagggtgcta aggcactggt
     2881 atgtaaagtc aaaggcaatg gcgtaaatca acatgttctg gcgatcctcg cagccgatca
     2941 acaggccgat ctgagccaac tcgccagcca tataggcgga ttacgcgctt cgttggccag
     3001 cccggcggaa gtcgatgaac tcacaggctg tgtcttcggc gcgatccccc ctttcagctt
     3061 ccatccaaaa ctcaaactgg ttgccgaccc tctactcttc gaacgatttg atgaaatcgc
     3121 cttcaatgca gggatgctgg ataaatccgt tattttgaaa accgcggatt atttgcgcat
     3181 tgcgcaacca gaactggtca atttccgccg cactgcgtaa ctagcctgtc cgttcaataa
     3241 gcagaacgga caaaaccagc acaacagcaa tggcaaaaaa cgatgacgtg ataatcagtg
     3301 tctcgacgaa catttgatcg ttcatagcat acccccggga gatagctgtt ttcacgttat
     3361 tgttagcgtg cacaaatggc agtttgatga cagttcgcct gattttttat gcaaaaaacg
     3421 taaagatttt ctacttcctt cctgcagcaa gcgtaaagta agcaggctta ttattttttg
     3481 gcaaggaaac cacgatgttt gatgtcactt tgctgatcct gctcgggtta gctgcgctgg
     3541 gctttatcag tcataacacc actgtcgccg tttcaattct ggtgttgatc attgtccgcg
     3601 tcacaccgtt aagcaccttt ttcccgtgga ttgaaaaaca ggggctaagt atcgggataa
     3661 tcattctgac gattggcgtt atggccccca tcgccagcgg gacgctaccg ccctcaacct
     3721 tgattcactc gtttctgaac tggaaatcgc tggtggcaat tgcagtaggc gttattgtct
     3781 cctggctggg tgggcgcggc gtgacgttaa tgggcagcca gccgcaactg gttgctgggc
     3841 tgctggtggg cactgtttta ggcgtagcgc tgtttcgcgg cgtaccggtt ggcccgctta
     3901 ttgccacggg tctggtttcg ctgattgtgg ggaaacagta gttaatcccg caatatatcg
     3961 gcctggcgtt tgccccaggc ctttcttaaa catagtaata aaggccgttg ttgaatcata
     4021 ccccagcgta tgagcaactt tctgtacagt atcgcctttc actaatccct gaagcgccat
     4081 aatcagctgt aactgctgtc gccactggcg aaaactcaac cccgtctctt ttactatcag
     4141 tcgggcaaga ttgcgttcac tcatcgcaaa aaagccagcc cattgcccca atgcccccca
     4201 ctcgacgggc tctttcgcca tcatctccac catcgcgcgg attttaggat gagaagaaac
     4261 gggtaggtgc agctgttgtt gtggctgctg agggagttca tcaaacagga cctgaattaa
     4321 tctgcgggtc atcggctcag cacgctgagt cgtcgtcctg tttgccagag ttaaaattaa
     4381 ttcccggcat aagggcgata ttttcaatgt gtaacatgtt gtcggcattg tcactgcgct
     4441 gggttcgata aacagaaaac agagttcagc gttagctgtc acttgattgc tatgttccac
     4501 cccgcccggt atccaaaccg catattgtgg cggcaccatc cacaaagcat tttccaccgt
     4561 acaggtaatt gcaccatgta gcgccagtat cagctgtcct ttgcggtgct gatgtacagg
     4621 actggttaac tcatccgttc cggcgtgaat acaaaatgcg accgcggctt catggtgtcg
     4681 gtcgggttca tagccattga gattcaacct gtgcatcatt ttgtccgaat ttagcgataa
     4741 tttgtcattt tagcttgatt caacataaca ataaaaacgg taaggtacag cctcgtttgt
     4801 aacaatgaga agcatatgac ctgttcaact tcattaagcg gcaaaaacag gattgtcctt
     4861 atcgctggca ttctgatgat tgccactaca ttacgcgtca ccttcaccgg cgcagcaccg
     4921 ttactcgata cgattcgttc cgcttactcg ctgacgactg cgcaaaccgg cttattgacc
     4981 accctgccat tattggcctt tgcgctaatc tcacctctgg ctgccccggt ggctcgacgt
     5041 tttggtatgg aacgtagcct gtttgccgcg ttacttttga tctgtgcagg tatcgcaatt
     5101 cgctctctcc cttcgcctta cttattattt ggcggtacag cggtcattgg cggtgggatt
     5161 gcattaggta atgtcttact gccaggatta attaaacgcg atttccctca ttccgtcgcc
     5221 agacttaccg gcgcatattc cctgacaatg ggagctgcgg cggctctggg atcggctatg
     5281 gtagtgccgc tggctttgaa cggttttggc tggcaaggcg cgttgctcat gctgatgtgt
     5341 tttcctctgc tggctctttt tttatggctg ccacagtggc gaagccaaca acatgcaaat
     5401 ttgagtactt cacgcgcctt acatactcgg ggtatctggc gttcgccgct tgcctggcag
     5461 gtaacattgt ttcttgggat caactcactg gtctattacg tgattattgg ctggcttccg
     5521 gcgatcctca tcagtcacgg ctatagcgaa gcacaggcgg gttcactgca tggtttgctg
     5581 caactagcca cagcagctcc cggtttgctg atcccacttt tcttacatca tgtgaaagac
     5641 cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag tgggcgcggt tgggctctgc
     5701 ttcatgccag cgcacgcgat cacctggact ctgcttttcg gttttggttc cggcgcaaca
     5761 atgatactgg ggttgacgtt cattggtctg cgggctagtt ctgcgcatca ggcggcggca
     5821 ctctcgggga tggcacaatc cgtcgggtat ttgttggcag cctgtgggcc gccgctgatg
     5881 ggtaaaatac acgatgctaa cggtgactgg tccgtaccac taatgggtgt tgcaatactt
     5941 tcactactga tggcgatttt cggactttgt gccgggagag acaaagaaat tcgctaatat
     6001 ccggtactat agtgacgtga caaatcatgc gtgaaaggga gaacaaacac gatgaatatt
     6061 cagtgcaaac gcgtttatga tccggcagaa cagagcgatg gttatcgcgt actggtcgac
     6121 cgcctctggc cacgcggtat caaaaaaacc gatttagccc ttgatgagtg ggataaagaa
     6181 atcacgccgt caacggaact gcgcaaagcc tttcacggcg aagtcgtcga ttttgcaacc
     6241 tttcgcgagc aatatcttgc agaactggca caacacgagc aagaaggaaa gcggctggcg
     6301 gacatcgcca aaaaacagcc gctgaccctg ctctactcag caaaaaacac cacgcagaac
     6361 catgcgctgg tgctggccga ctggctacgt agcttgtgat tttagtacag catccggcgg
     6421 ttatttttca ccagccggat ggtccagtcg ccacaatgcc cattcatcaa tcgtttcacc
     6481 gcccggtaat ttgcaattgt tgctgacccc ttgcgctgtc tgcactggaa tgagcgtccc
     6541 gcccttctgc tggcaataga ccgacgccgg atttgccata ccaatctgcg gcggtttagg
     6601 tgcttctggc tgagaagggg ttgaacaacc agccaggacc agcaagcaag gcagaacaaa
     6661 actgataatt ttcatttatt gatctcacat atgtatccaa gcttagagta tcgcggtatc
     6721 gttttgtttt gcagcactat ttttattaca ttcactcaaa acatattacg tcttgtttca
     6781 tctttgttga tgatgtttta tcatgcctgc aaagattaaa taatcagcat ttacccgccg
     6841 tatcctggag ttgtttcgtg tcagatcaga ttatcgcccg catctcgcaa tcccttgcca
     6901 aagaacagtc actggaaagt ctggtccgac agcttctgga gatgctggaa atggtcactg
     6961 atatggaatc aacctacctg accaaagtgg atgtcgaagc gcgcctgcag catattatgt
     7021 ttgcccgtaa cagccagaaa atgcacatcc cggagaattt taccgtctcg tgggattact
     7081 cgttatgcaa acgcgccatt gatgaaaact gctttttcag cgatgaagtc cctgaccgtt
     7141 ggggcgactg tattgcggca cgcaatcttg gcatcaccac atttctgagc acgccaattc
     7201 acttaccgga tggatcattc tatggcacgc tttgcgccgc cagcagtgag aagcgccagt
     7261 ggagtgaacg cgcggaacag gttttacagt tattcgccgg actgattgca caatatattc
     7321 aaaaagaggc actggttgaa cagctgcgcg aagccaatgc tgcgctgatt gcgcaatcgt
     7381 ataccgactc gttaaccggg ctaccgaatc ggcgggcgat ttttgaaaat ctgacgacgc
     7441 tgttttccct cgcccggcat cttaaccaaa agataatgat cgcgtttatc gatctggata
     7501 acttcaaatt aatcaatgat cgttttggtc ataatagtgg cgatctgttt ctcattcagg
     7561 ttggcgagcg ccttaatacg ctccagcaaa atggcgaagt tattggtcgt ctcggcggtg
     7621 atgagttttt ggttgtttca ctaaacaacg agaatgcgga tatttcgtcg ctgcgagaac
     7681 gcattcagca gcaaatacgc ggagaatatc acttaggtga tgttgatttg tattatcctg
     7741 gtgccagtct tggcatagta gaagtcgatc ctgaaacgac cgatgcagac agtgccctac
     7801 atgctgccga tatcgcgatg tatcaggaga aaaaacacaa acagaaaaca ccttttgtcg
     7861 cgcattcagc gccacattcc tgaggcgtat tcacatcctt ttgattggtg ataacatgcg
     7921 aatcggtatt atttttccgg ttgtaatctt cattacagcg gtcgtatttt tagcatggtt
     7981 ttttattggc ggctatgctg ccccgggagc ataaagatga aaaaaacaac gatcattatg
     8041 atgggtgtgg cgattattgt cgtactcggc actgagctgg gatggtggta acgtcacatc
     8101 taaaaaatag caaaggctgc ctgtgtgcag cctttgtgca atttacgcgt taacttttaa
     8161 tcttcctgta gataaatagc acgacaatcg caccaataac ggcaaccacg aagctgccaa
     8221 aattgaagcc atcgacttta ccaaagccaa acagcgtgct gatccaaccg ccgactacgg
     8281 caccgactat ccccagcagg atagtcataa agaatccacc accatcttta cctggcatga
     8341 tccacttcgc cagaataccg gcaataagcc caaaaataat ccatgacaga atgcccattg
     8401 tttcctcact tatctgtttt gcattagcgg gttagtcgct gataaaaagc atagcacaac
     8461 atcgggaggg caagatttgt gacgagcatc acggaggttt ttttgcgatg gcgcagaaat
     8521 tgcgccatca acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc
     8581 ataagaacgc acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag
     8641 caaagtcatc ggcgtctctg tttcataatc gacagaaacg ccattgctgt tattggtgac
     8701 ggtcacggta tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg
     8761 ttgcgcacct tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc
     8821 gtcagtcatg gcttcaatgt tgtgccactt ttctggaggg aacaccgcaa actgccccgc
     8881 ttcgataagg atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt
     8941 gaccgcccca tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag
     9001 atgacgttcg aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt
     9061 atgaatataa ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa
     9121 aacggtttat tccagccgtt tcagggtacg cctgataatt tgctttttaa ataccattta
     9181 ttggttactt tttagcacca tatcagagaa aaatcaggga ggattataga tgggaagccc
     9241 gcgacaacgc gggctgaaag catcaggatt gcagcgtcgc cagtcgggca gcgaaaccca
     9301 cgaacatcaa accaatcagt gagttgccca ctttagccag tttctttttg gtacgtatgt
     9361 actgcgtgac aaaagcacca gatataatca ggaagctcaa atagcagaaa ctcaccagtt
     9421 ccagcgtcgt cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa
     9481 actgtacgaa aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta
     9541 acgcgcgttt aaaaatagca ccgtattggg gctcatcgga tttggcctcg ttatttttac
     9601 ccttcagggt cgcgtaaaga attttactcc ccagatagag caaataaaac gcaccaagat
     9661 aacgtacaat gttgaataat atcggggtgg tcttaattaa tgtcgccatt ccagcccatg
     9721 ccagaaacat caataccgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt
     9781 tcataccgct actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca
     9841 caataaaaat ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca
     9901 cattaacctc tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat
     9961 ctgtagcggc ttagaaagga gaaaatcagg ttttaacctg atatcaaccc gataattgaa
    10021 tcattaatca ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta
    10081 aaaattctac cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc
    10141 agacgttcgc tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt
    10201 tttcgctaac atcccaccag gaacgcagag caatcccctg cccatcgaga caccactgat
    10261 ggacaatttc tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt
    10321 ctttattgcg taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg
    10381 gcaatgcaga aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg
    10441 acgcgcagag aatgcggtaa ttggtcgcca gtttgcgggc gattaaatta ggggcgatgt
    10501 catcgccaat gcgaatatcg agatcgacac cttcattgac caaatcgacc agccggtctt
    10561 ccacatcaaa acgtaattcc agttgcggat acgccttcgc cagcgctgat aacgccggag
    10621 ccaccacctg tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgtggcacct
    10681 gacgcacgtc agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc
    10741 attcataaat ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca
    10801 acaccacgtt tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa
    10861 cgcccagttc ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc
    10921 gcaaatcatt cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg
    10981 gggattaatt ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg
    11041 aagtgagaac ccaatgatga aaacgatgcg tattgctgcg atcccgggag acgggattgg
    11101 caaagaagtc cttcctgaag ggattcgcgt gttacaggct gccgctgagc gctggggctt
    11161 cgccttgagt tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa
    11221 aatgatgccg gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc
    11281 cgtcggctgg ccggacaccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt
    11341 tcgtcgtgaa ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc
    11401 ctgcccgctg gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac
    11461 cgaaggcgaa tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt
    11521 cattcaggaa tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga
    11581 acttgcgcaa agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc
    11641 catcagcatg ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat
    11701 ccgctgggac aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg
    11761 cttcgatgtg gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc
    11821 ctgcaccggc accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc
    11881 ctcgctcttc gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa
    11941 ccctatcgcc acaatttggg ccggggcaat gatgctcgat tttctcggca atggcgatga
    12001 gcgtttccag caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg
    12061 gccgaaaaca ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg
    12121 caaaattatt ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt
    12181 tgttattcgt tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc
    12241 gtgttttatg gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat
    12301 tcgttaaaca cgacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga
    12361 gacgggcttt tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc
    12421 cgctgataag catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc
    12481 cacaacaatc ggccgacgcg gccaacgcaa tatacacttt tgttacgcgt acgttaggtt
    12541 ccgcagtaca ggtattggtt ttgctggcaa tgggactagt gatttattta gccaccagta
    12601 aatacggcaa tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt
    12661 ttatgtttat ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg
    12721 cctattatta tcaaacgcct ggattaaata tcgcaccgcg ttcacaacag gcactcgaat
    12781 ttagcgtccc ttactccttc ttccactggg gcatcagcgc ctgggcaact tatacgctgg
    12841 cctcattaat catggcttat cactttcatg tgcggaaaaa caaaggtctg agtctttccg
    12901 gcattattgc cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt
    12961 tgatgttcct gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa
    13021 cctttactcg tggactttcc gcactgaccg gtttacccga taacttcacc gtgcaggcat
    13081 ttgtgatcct gctttccggc ggcatttttt gcctaagctc atggattggt atcaacaacg
    13141 gtttgcaacg tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc
    13201 tgattgtcgg tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca
    13261 cgcaaaactt cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc
    13321 gcaactggac cgttttctac tggctgtggt ggatctcata cacccctggc gtagcaatgt
    13381 ttgtcacccg cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg
    13441 gcagcaccgt cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt
    13501 ttatcaatgg cgtaatcaac gtcccacagg tgctggaaac tctgggcggc gaaacagctg
    13561 tacagcaagt tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg
    13621 tgatgattat tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca
    13681 gtacgcgtaa tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg
    13741 tggtgatcac tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga
    13801 aaaccaccgt cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg
    13861 gatttattcg ctggctgaaa caggattacg ccgacattcc ggctcatcaa gttgaacatt
    13921 atctcccgca gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg
    13981 tattcaaagg cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat
    14041 ctgagccctg actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc
    14101 attcctgccc gtttttatac cgatcagaac gcctttgaac acgaaaaaga gaacgtcttc
    14161 gccaaaagct ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg
    14221 acgcgtgaga tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc
    14281 gcgttctata acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca
    14341 aaaaatgtga ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc
    14401 catgcacgta actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg
    14461 gtgcgtctgg aagaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc
    14521 gtagaagatc agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac
    14581 gatctgaaac tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc
    14641 gataactatc tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta
    14701 caggttgatc gttactggca caccatgcac ggtaactgga cgctgcaata cagtttcgcc
    14761 aaaccgtccg aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc
    14821 tggctgtggc cgtgcacgat gctgaatgtc accccgatca aagggatgat gacggtcatt
    14881 tatgaattcc cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat
    14941 gaagagttaa ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg
    15001 gaagatttac gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt
    15061 caggggcgca tcatggccga cagtagcggt agcggcattt ccgaacatgg tatcgcccat
    15121 ttccataatc tgctggcgca ggtatttaag gactaatgac atcggcggcg gtattttccg
    15181 ccgccgggct gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca
    15241 ggtgagtcag gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgccgg tggcaaccga
    15301 tggcaaaccg ctgcctgcgt tcaccggagg aagtcacatc atagtgcaga tgagcgatgg
    15361 tgataaccag tacagtaatg cgtattcact actgagttca ccgcataaca cctctagtta
    15421 tcagattgcc gttcgactgg aagaaaactc gcgcggcggt tcccgctttt tgcatcagca
    15481 ggtaaaagtg ggcgatcggt taacgatctc aacgcctaat aacctgtttg cgctaattcc
    15541 ctcagccaga aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca
    15601 catggcagag ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc
    15661 agaaagttgc gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt
    15721 gcatcattca tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc
    15781 tggcacacac gtttatacct gtggccccga ggcgctaatt gaagcggtaa gaagtgaagc
    15841 tgcgcgtctg gacatcgctg ccgatacgct gcactttgag caatttgcta tcgaagacaa
    15901 aaccggcgat gcatttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga
    15961 agagatgact attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg
    16021 tcgtgaaggg gtatgcggaa cctgcgaaac ggcaatactg gaaggtgaag ctgaccatcg
    16081 ggatcagtat tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc
    16141 gcgtgcgaag ggtaaacgcc tggtgttgga tttgtagttt gccgaagccg gatgtggcgc
    16201 tgagcgcgcc cagtccggct tcggaaaatt ttactgcgga tattcctgca ataaattgtg
    16261 taatgcttcc gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg
    16321 cggtttcagt ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa
    16381 ttcggcgctg atcttgtgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa
    16441 cgctttacga taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc
    16501 cggcaatgtc tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc
    16561 gctaccggat aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgctac
    16621 cgaccacaaa tgctcttcac gcacgacaaa gttcaccgcc agatcgcgct ctcgcgcctt
    16681 gcgcagtcgc cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca
    16741 ggcattggtg atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg
    16801 catcaggcga cattcatcca gcgccgctgg tagccagccg gaggcctccg tttctaccat
    16861 aagcttggcg gtgatcggta acagatacca gacatccgcc gctgcatatt cacactgacg
    16921 ttcggtcagc ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc
    16981 ggaatactct tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc
    17041 aaggatttgc gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc
    17101 cagatcttca ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt
    17161 cagcggtgac cagtcggtga tcccgagagg atcaattagc gccagatgct cgccatcgaa
    17221 aagttgaatc aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatccag
    17281 ggctatcgcc ggaaaggcac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt
    17341 aatcatttgg taattcaaat tgttttctct ttagtgggcg tcaaaaaaaa cgccggatta
    17401 accggcgtct gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc
    17461 gtcacgtaat tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa
    17521 ctccaccagc ttcggtactt tgtagcccgt gagctgacgg cggcaaaagg tcaccagtga
    17581 ctcttcggta agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga
    17641 gccggaaggt acaccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc
    17701 ttcaatctcg ttgggataga cgttaaaacc ggaaaccaaa atcatgtctt ttttacgatc
    17761 gacaatgcgc aggaatcctt cttcatccat taccgcgatg tcgccagtgt gtaaccagcc
    17821 gtttttgatg atttcatcgg tagcatccgg acgctgccaa tagcccagca tcacctgtgg
    17881 acctctgacg caaagttcac ctggctgacc tggcgatact tcattatcgt cgtcatccac
    17941 cagtttggct tccgtcgacg gcaccggcaa accaatgcta ccgctatgat aatcaatatc
    18001 ataggggttc acgctgacca gcggcgcaca ttcggtcagg ccataacctt ccagcagata
    18061 ctgtccggtc agtttcaccc agcgttctgc caccacttgc tgaacaggca tcccaccgcc
    18121 tgcagaaagg tgcagactgg agaaatccag ttgttgaaac tctttattgt tcagcaacgc
    18181 gttgaacaag gtgtttacgc cggtgatagc ggtaaacgga tatttcgcca gctctttgac
    18241 cagccccgga atatcccgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa
    18301 cagcaggcag ttaatggtca gtgcaaaaat gtgatacagc ggcagcgccg tcaccaccag
    18361 ctctttgccc ggatgcagca gaggcccata cgtagcgtta acttgttcca ggttcgccag
    18421 catattgcgg tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cagtgtattg
    18481 caggaaggct aaatcttctg gcaccagttc aggtttgaca tactgcatac ggtagccgtt
    18541 gtgcagggcg ctacgaaatg aaatggcatc tggcagatga tatttcggca ccagacgttt
    18601 gatgtattta acaacgaagt taaccagtgt gcctttcgct gtggaaagct gatcgcccat
    18661 acgggtcagg ataacgtgct gaactgcggt tttatcgacc actttttcca gcgtgtgagc
    18721 aaagttggac acaatgacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc
    18781 acgcggggta tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa
    18841 cagcgccacc ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt
    18901 cagccccaac ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg
    18961 gaaggtcatc acctccccca tattcacaaa cgcaggctga tcggcgtagc gcgcgaccga
    19021 ctgctcaaac atatctacca gagattgata acggtcaggg ttgatctccg tcggaacgtc
    19081 cgcgggataa cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt
    19141 cgtcatcgca accccaaatg atatacatgc cgttaacata atattaactc atcataccag
    19201 cttgataatt acccaacgaa aaggttgcga agcgcgtcac tatttatttt tatctttacc
    19261 gtaagaatgc agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caagcaatta
    19321 ttcagttaca actgtttgta ctctcgcggg gccgggatta taccagcccc atccgccgtg
    19381 gccatagggc cagccacgac cgccataaaa ccagggatca atcggctgag gcggcataat
    19441 cacctgctgg gttaaatgcc aacgtttgta acctgttgcc tgcatcacca taaatttata
    19501 gggcgtgttg ccgatttttc cgtcaaccgc accagtgatt ggcccgacta ccgtaaccag
    19561 ttgtccacgg aaatccaccg ggtccagaaa accgttcaca tcggcataaa tgcgaccgcg
    19621 agaaggttct cccagcgtcg gtctggctcc gctgtccagc ggtacggtag caatttccag
    19681 gcgggttttc ccttgctggt tttgtaccgc aaccactttg ccaccaaagc gtgcctcctg
    19741 accaacgtac agctgcggcg cactcatcac ccgaactaaa tcttgttgcg gcgtgggact
    19801 gctgccttta atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc
    19861 tgccagtatg cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc
    19921 agggcatact cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg
    19981 taaataaacc ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca
    20041 agcaatcggc agcatatctt ctgcagcggg cagtaaaact tctccgtcgc gcaaaaccag
    20101 tccgctctct ttaccgagat ccggccaggc ttgccagcct gtacctaccg tcacccattc
    20161 cccggaaagc tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc
    20221 ttcaccgtgc cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat
    20281 tcgcgcgtca atggctgaca gcacgcgtgt tgcgccgttt ttgcgccacg ccccttgcgc
    20341 catcgtcatt agcgtggaga cgccaatcat cggtaattcc gcgccaagcg ccagcccttg
    20401 cgcgatgcca ataccaatgc gcacgcctgt aaagctaccg gggccgcgcc cgtaagccag
    20461 agcgttaata tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa
    20521 gattcgttga gtatgttcac gagggcaaag ctcaaaatga gcgttgacag taccgtcgtt
    20581 ccacagggcg acagagcagg cctctgtcgc ggtatcgata gccagaattc gcatgggtct
    20641 tcgtgcttag atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga
    20701 ggatggtatc gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg
    20761 cagactggcg agaaacgtcg cgccgtaagg acgcatcacc agccgattgt cacaaattac
    20821 caaaaccccg cgatcgtcgg cgtcgcgaat cagtcgcccg actccctgct tgagagtaat
    20881 caccgcatcc ggcagttgta cttcatcgaa cgggtcgcca ccgcgcaaac ggcaatcttc
    20941 catgcgcgct tttaacaatg gatcatcagg cgaggtaaac ggcaatttgt cgataatcac
    21001 caatgacaat gtatcgccac gcacgtccac cccctcccag aaactactgg ttgccacaag
    21061 aagcgcgtta ccggcactga caaattgctg caacagttgc cctttgctgg tttccccctg
    21121 caataatacg ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc
    21181 gtgcgaggta caaagcataa aacagcgacc gttgttagct tcgatgatcg ggcgcagcat
    21241 agccgccagt tgccgcgcgg aacccggttg attggtttgc ggcaggttgc gcggcacaca
    21301 gagtaacgcc tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc
    21361 tatcccaagc cgcgaggtga aatgatgcag atcgtcgttt accgacagcg ttgctgaggt
    21421 gaagatccag ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag
    21481 cggcgtgaga gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg
    21541 ctgattgatc tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc
    21601 cgcatccagc aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccag
    21661 ggtgtcatcg agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag
    21721 gttaccgcga taacctggtt caccgagttg cagacgaaaa tcctgcgcac tctgggcaag
    21781 acgatcagcg cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt
    21841 gatgtctttt gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct
    21901 ggcaatgtcc ggtagctggt gggcttcgtc gaagatcatg acgtccgctt ccgggatcaa
    21961 ctcgccaaat ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac
    22021 caccacatcg gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat
    22081 cgggcagtcg ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgcga
    22141 atcttccgcc acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga
    22201 gcgcagcagg atcacatcgc ttaatatttg taccggaaga tcgccccccg ccagcgcctg
    22261 ctgttcgaga cgttcgaggc agaggtagtt tgagcgccct ttcagcaacg ccacgttgcc
    22321 cgtgtatttc aacgcctttg agaccgtcgg caaatcgcgg ctatagagct gatcctgcaa
    22381 cgcttttgag ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gggccaggta
    22441 agcgtaggtt ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat
    22501 cgcctgggtg acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat
    22561 cgctttcgcc agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt
    22621 gaacagggat tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcgacctc
    22681 acattgtggc agtctttgca gcacgaaaat ggaaggataa tgaggataaa atgatgacta
    22741 tcgttcgtat cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct
    22801 actacactgg tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacaccc
    22861 tggcgcagat tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg
    22921 ccaccatttt cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct
    22981 gggttgtcgc gggtcatgcg ccggtgcgct gcacggtaca agcgggtttg atgaacccga
    23041 agtataaagt tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt
    23101 cagacgataa tttatcgata atactggtcg gttatacata aatcgaccag aga
//
LOCUS       DAZKFZ010000061        22663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000061 DAZKFZ010000000
VERSION     DAZKFZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..654
                     /gene="ydcL"
                     /locus_tag="ACHOQW_002142"
     CDS             <1..654
                     /gene="ydcL"
                     /locus_tag="ACHOQW_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP9573222.1"
                     /translation="FAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKETTSATG
                     KPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQ
                     RKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLY
                     FEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
     gene            complement(689..1900)
                     /gene="ydcO"
                     /locus_tag="ACHOQW_002143"
     CDS             complement(689..1900)
                     /gene="ydcO"
                     /locus_tag="ACHOQW_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP9573223.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMVSQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            1953..2489
                     /gene="sutR"
                     /locus_tag="ACHOQW_002144"
     CDS             1953..2489
                     /gene="sutR"
                     /locus_tag="ACHOQW_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP9573224.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2562..4523
                     /gene="rlhA"
                     /locus_tag="ACHOQW_002145"
     CDS             2562..4523
                     /gene="rlhA"
                     /locus_tag="ACHOQW_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP9573225.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4615..4845)
                     /gene="yncJ"
                     /locus_tag="ACHOQW_002146"
     CDS             complement(4615..4845)
                     /gene="yncJ"
                     /locus_tag="ACHOQW_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP9573226.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            5067..5243
                     /gene="hicA"
                     /locus_tag="ACHOQW_002147"
     CDS             5067..5243
                     /gene="hicA"
                     /locus_tag="ACHOQW_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP9573227.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5289..5705
                     /gene="hicB"
                     /locus_tag="ACHOQW_002148"
     CDS             5289..5705
                     /gene="hicB"
                     /locus_tag="ACHOQW_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP9573228.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5784..7190
                     /gene="ydcR"
                     /locus_tag="ACHOQW_002149"
     CDS             5784..7190
                     /gene="ydcR"
                     /locus_tag="ACHOQW_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9573229.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            7435..8580
                     /gene="ydcS"
                     /locus_tag="ACHOQW_002150"
     CDS             7435..8580
                     /gene="ydcS"
                     /locus_tag="ACHOQW_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP9573230.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8598..9611
                     /gene="ydcT"
                     /locus_tag="ACHOQW_002151"
     CDS             8598..9611
                     /gene="ydcT"
                     /locus_tag="ACHOQW_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9573231.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9612..10553
                     /gene="ydcU"
                     /locus_tag="ACHOQW_002152"
     CDS             9612..10553
                     /gene="ydcU"
                     /locus_tag="ACHOQW_002152"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9573232.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10543..11337
                     /gene="ydcV"
                     /locus_tag="ACHOQW_002153"
     CDS             10543..11337
                     /gene="ydcV"
                     /locus_tag="ACHOQW_002153"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9573233.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11359..12783
                     /gene="patD"
                     /locus_tag="ACHOQW_002154"
     CDS             11359..12783
                     /gene="patD"
                     /locus_tag="ACHOQW_002154"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP9573234.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12880..12975)
                     /gene="yncL"
                     /locus_tag="ACHOQW_002155"
     CDS             complement(12880..12975)
                     /gene="yncL"
                     /locus_tag="ACHOQW_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP9573235.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            13170..13343
                     /gene="ortT"
                     /locus_tag="ACHOQW_002156"
     CDS             13170..13343
                     /gene="ortT"
                     /locus_tag="ACHOQW_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP9573236.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13429..13662
                     /gene="ydcY"
                     /locus_tag="ACHOQW_002157"
     CDS             13429..13662
                     /gene="ydcY"
                     /locus_tag="ACHOQW_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP9573237.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13663..14112)
                     /gene="ydcZ"
                     /locus_tag="ACHOQW_002158"
     CDS             complement(13663..14112)
                     /gene="ydcZ"
                     /locus_tag="ACHOQW_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9573238.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(14109..14627)
                     /gene="mddA"
                     /locus_tag="ACHOQW_002159"
     CDS             complement(14109..14627)
                     /gene="mddA"
                     /locus_tag="ACHOQW_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP9573239.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14808..15845
                     /gene="curA"
                     /locus_tag="ACHOQW_002160"
     CDS             14808..15845
                     /gene="curA"
                     /locus_tag="ACHOQW_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP9573240.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            16043..16708
                     /gene="mcbR"
                     /locus_tag="ACHOQW_002161"
     CDS             16043..16708
                     /gene="mcbR"
                     /locus_tag="ACHOQW_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP9573241.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16744..18846)
                     /gene="pqqU"
                     /locus_tag="ACHOQW_002162"
     CDS             complement(16744..18846)
                     /gene="pqqU"
                     /locus_tag="ACHOQW_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP9573242.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            19088..20149
                     /gene="yncE"
                     /locus_tag="ACHOQW_002163"
     CDS             19088..20149
                     /gene="yncE"
                     /locus_tag="ACHOQW_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP9573243.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20264..21763)
                     /gene="ansP"
                     /locus_tag="ACHOQW_002164"
     CDS             complement(20264..21763)
                     /gene="ansP"
                     /locus_tag="ACHOQW_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP9573244.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            22030..22647
                     /gene="yncG"
                     /locus_tag="ACHOQW_002165"
     CDS             22030..22647
                     /gene="yncG"
                     /locus_tag="ACHOQW_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP9573245.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEII"
BASE COUNT         5777 a         5565 c         5834 g         5487 t
ORIGIN      
        1 tttgcgaaag ttgcagcttt atgcggcttg ttggctctgt ctggttgtgc ctctaaaatc
       61 acccagccag ataaatattc tggtttttta aacaattact ctgatttaaa agaaacaacc
      121 tcggctacag gtaaaccggt tttacgttgg gtagacccga gttttgatca aagcaaatat
      181 gacagcatcg tctggaaccc aatcacttat tatccggtac cgaaaccttc gacccaggta
      241 gggcagaaag ttctggataa aattttgaac tataccaaca ccgaaatgaa agaagcgata
      301 gcgcagcgta aaccactggt taccaccgcg gggccgcgta gtctgatttt ccgtggggcc
      361 attaccggtg tagataccag caaagaaggg ctgcaattct atgaagtggt tcctgttgca
      421 ttagtggttg cggggacgca aatggctaca ggccaccgta ccatggatac tcgcttgtat
      481 tttgaaggtg agctgattga tgcagcgact aataaaccgg ttatcaaagt cgttcgtcag
      541 ggcgaaggta aagacctgaa taacgaaagt acgccaatgg ctttcgaaaa tattaaacaa
      601 gttattgatg acatggcgac cgatgccacc atgtttgacg ttaacaaaaa gtagttccag
      661 acgcgccatc gttagatggc gctttttatt atcggtttct gtcagcgatt aaattcaaca
      721 ccacgtaaca aacgcctccg gcaattaatc cccaaaacgc agaaccaatc ccgaccagcg
      781 tcaatccact tgccgttacc agaaatgcca ccaccgccgc gtcgcgctca cgctcattat
      841 gcagcgcctg atacaaactg ccgccaatgg tactcaacag cgccagacct gccagcatct
      901 ggatccaact tacgggcagg gcagccatca tcccggtaat ggcactacca aacagacctg
      961 cgagcaaata gaaaatgcct gcaacggcag ctgccagcca acgttgatct ttatccggat
     1021 gcgcttccgg gctttggcaa atagccgcgg tgattgccgc aataccgacg gaataaacgc
     1081 cgaaagggga aaaaaccagt gccagcaatc cagtaaacac aatcaacggc gaaacaggag
     1141 ccgaataccc ggctgctttc attgctgcga tacccggtgc gttttgcgat accatcgtca
     1201 ccagaaaaag ggggagtgca acgctcaggc tgtgagcaaa cgaaaaatca ggggtaatat
     1261 aagtggggag aacgggttta aagacaacat cagttgtgac aacgtcacct tgcgcgataa
     1321 cgatcacgat cccaataatc atcgcggcaa ttaccgcata gcgcggcgca acggccttgg
     1381 ttgccagcca taccagcaac atacttccac acaacgtaaa ttgaccgtcc agactggcaa
     1441 acgcctgtaa accaaagcgt aataaaatcc cggcaagcat tgctgccgca agcgagtgcg
     1501 gaataatgcg catcagacga gcaaagagtc ccgttatgcc gcagaggact attaacgcgt
     1561 tggtgacaat aaaaacgccg atggcttcgt taagtgttag tccctgcaat ccggtgacca
     1621 acaaagccgc gccaggcgtt gaccatgcgg tgagaacagg tacgcgatac cataatgtca
     1681 gagtcagcgt actgacgccc attgccagcc ccagcgccgt catccagcca gagatttgtg
     1741 cagtggtggc tccggcgaca atcgctgctt gccagattat tgccgctgaa ctggcgtagc
     1801 caattaatac cgccagaaac cccgccagta gcgtgggtgg aggaacagaa aacagacgca
     1861 tagtcacctc gtgcgttata gcgtacaggc accgtagcat ttgtccgtta taacgcacaa
     1921 gtgataaact tccgttttgc cggaggagtc gcatggaaaa tctcgctcgc tttttatcca
     1981 ccacacttaa acaactacgt cagcagcgcg gctggagtct ttcgcgactg gcagaagcga
     2041 cgggcgtttc taaagcaatg ttaggacaga ttgagcgtaa tgagtccagc ccgacggtcg
     2101 cgacattatg gaaaattgcc actgggttga acgtgccatt ttcgacattt atttctccgc
     2161 cacagtccgc tacgccttca gtttatgatc cacaacagca ggcgatggtg atcatttcgc
     2221 tgtttcctta tgatccacaa ctctgctttg aacacttctc gattcagatg gcacccggtg
     2281 caataagcga atcgacgccc catgagaagg gggtaatcga acatgtcgtc gtcattgatg
     2341 gacaacttga tctgtgcgtt gatggtgaat ggcagaccct taattgtggg gaaggcgttc
     2401 gatttgctgc agacgtcacg catatctatc gtaatggtgg ggagcaaacc gtacattttc
     2461 attccctcat ccattacccg cgcagttaag cgggaaaact atttcgcaac gtcgcgcttg
     2521 tggctaaaat agccgccatt tttcagctac tggataagaa tgtgaccgta tcttctcatc
     2581 gacttgaact gttaagcccg gcacgcgatg ccgccattgc ccgcgaagct attttgcacg
     2641 gtgccgatgc tgtttatatc ggcggccctg gttttggtgc ccgtcataat gccagtaata
     2701 gcctgaaaga tattgccgag ctggtgccgt ttgcccatcg ttatggtgca aaaattttcg
     2761 tcacgcttaa caccattttg catgatgatg agctggaacc cgcgcaacgg ctgattactg
     2821 acctctacca gaccggtgtc gatgcgctga ttgttcagga tatggggatt ctggaacttg
     2881 atattccgcc gattgaactg cacgccagta cgcagtgcga cattcgtaca gtagaaaaag
     2941 cgaagttcct ctctgatgtt ggcttcacgc agattgtgct ggcgcgagag ctgaatcttg
     3001 atcagatccg cgcgattcac caggccacgg acgcgaccat tgaattcttt attcatggcg
     3061 cactgtgcgt ggcctattcg ggtcagtgct acatttctca tgcgcaaaca gggcgtagcg
     3121 ccaaccgtgg cgattgctcg caagcgtgcc gtttgccata cacattgaaa gacgatcagg
     3181 ggcgggtggt ttcctatgaa aaacatctgc tgtcgatgaa agataacgat cagactgcca
     3241 acctcggcgc gctgattgat gctggcgtac gttccttcaa gattgaaggg cgttacaaag
     3301 atatgagcta cgtgaagaat atcaccgccc attatcgcca gatgcttgat gccattattg
     3361 aagaacgtgg cgatctggcg cgcgcttcat caggtcgtac tgaacatttc tttgttccat
     3421 cgacggaaaa gactttccac cgtggtagca cagattattt tgtgaatgcc cgtaaaggcg
     3481 atattggcgc gttcgattcg ccgaaattta tcggcctgcc ggtaggtgaa gtagtgaaag
     3541 tggcgaaaga tcatctcgat gttgccgtta ccgagccact ggcaaatggc gatggcctga
     3601 acgtgttgat taaacgtgaa gtcgtcggtt ttcgtgccaa tacggtcgag aaaaccggag
     3661 aaaatcagta ccgcgtctgg cccaatgaaa tgccagcaga tttgcacaaa attcgcccgc
     3721 atcacccact aaaccgtaat cttgatcata actggcagca ggcactgaca aaaacctcta
     3781 gcgaacgtcg ggtggcggta gacattgaac tgggcggttg gcaggaacaa ttgattctga
     3841 ctctcaccag tgaagagggg gtcagcataa cgcatacgct ggatgggcag ttcgacgaag
     3901 ccaataacgc agaaaaagca atgaacaatc tgaaggatgg tctggcaaaa ctggggcaaa
     3961 ccctctatta cgcccgcgat gtgcaaatta atttgccggg ggcgctgttt gtaccaaaca
     4021 gtctgttaaa ccagttccgc cgtgaagctg ccgacatgct ggatgctgcg cgtcttgcca
     4081 gttaccagcg cggcagccgt aaaccggttg ctgatcctgc gccggtttat ccgcaaacac
     4141 atctgagttt cctcgcgaac gtttacaacc agaaagcgcg tgaattttat catcgctatg
     4201 gtgtgcagct gattgacgcg gcgtatgagg cacatgaaga gaagggcgaa gtcccggtga
     4261 tgatcaccaa gcattgtctg cgctttgcct ttaatctgtg cccgaaacag gcgaaaggca
     4321 atatcaaaag ctggaaggcg acgccaatgc aactggttaa cggcgatgaa gtattaacgc
     4381 taaagtttga ttgccgccca tgcgagatgc acgtcattgg caaaatcaaa aatcacatac
     4441 tgaaaatgcc gttaccggga agcgtagtgg catccgtaag tccggatgag ctgctgaaaa
     4501 cattgccgaa gcgaaaaggg taaaacgcca gttttctggt tactcacaac ttattgaatc
     4561 tgcatgatat tgcctgccgg gtaaggcgtt acgccgcatc cggcatcaaa cgactcaacg
     4621 ttgtcccgct tctggtttgc gcgatttttg ccagtaaaaa tgctcgcgca aaccttccgc
     4681 cgactcttcc gccacagcac gcaattcatc gctgtccgct tcatgacgca gctgatgatc
     4741 cacattcttt acccacacaa attcatgtcc tttgtgccct gccatgagtt gtcctgagaa
     4801 cagcgcacac gttaataaga caaccgataa cgccttcgta aacatcctgc cacctttttc
     4861 ttaccttttg ccgctatgat gccgatcgtt tcttgaggtt attattcagt tttgcaaatt
     4921 agcgcaaaga aattctggaa tcttccttcc tgattttgca ttgcattctg ccgttgcggc
     4981 gatttagtgc tattttcgag caaattacac acggaggtaa acactaatgt ttactttgtt
     5041 gatatactca gccgcaggga ggcgatgtga aacaaagcga gttcagacgt tggctcgaat
     5101 ctcagggcgt cgatgtagcg aatggcagca accatttgaa actcaggttt catgggaggc
     5161 gcagtgtcat gccgcgtcac ccctgcgatg agattaaaga accattgcgt aaagcaatcc
     5221 tgaaacaact cggtttgagt taatcgccaa ttaaaaaggt taatgacatg cgagagacag
     5281 tcgaaattat gcgttatccc gtcactctta cacccgcgcc ggaaggcggt tatatggttt
     5341 cttttgtgga tatccctgaa gcgttgacgc agggcgaaac tgtcgctgaa gcgatggaag
     5401 cggcaaaaga tgctttactg accgcatttg atttttattt tgaagataac gagcttatcc
     5461 ctttaccttc gccattaaat agtcatgatc actttattga agtacctttg agcgtcgcct
     5521 ctaaggtatt gctgttaaat gcttttttac agtcagaaat cactcagcaa gagttagcca
     5581 ggcgaattgg caaacctaaa caagagatta ctcgcctatt taacttgcat catgcgacaa
     5641 aaatcgacgc cgtccagctc gcggcaaagg cgcttggcaa agagttatcg ctggtgatgg
     5701 tttaattaca gttaacgaaa agttgtcatt tttaacaact gatatagact gccgaatcat
     5761 ctgcacataa ttacgattcg ataatgaaaa aataccagca gcttgcagaa caattacgcg
     5821 agcagattgc gtcgggtatc tggcaacccg gcgatcgttt accttcgttg cgtgaccagg
     5881 tggcgctttc tggcatgagc tttatgactg tcagccatgc ctatcagttg ctcgaaagtc
     5941 agggatatat tatcgcacga ccgcagtcgg gttattacgt tgcgccacag gcaataaaaa
     6001 tgccgaaagt gccagtcatt ccagtcactc gagatgaagc agtcgatatc aacacttata
     6061 tttttgatat gttgcaggcc agtcgcgatc cgtcggtcgt tccgtttgcc tcggcctttc
     6121 ccgacccgcg acttttcccc ctccaacaac taaaccgctc gctggcgcag gtaagcaaaa
     6181 ccgccacggc gatgagcgtg attgaaaact tgccgccagg aaacgcagaa ctgcgtcagg
     6241 ctattgcccg tcgctatgcc ttacagggga tcaccatttc tcctgatgaa attgtcatca
     6301 ctgccggggc gttagaggca ttaaacctca gtttgcaagc ggtaactgaa ccgggcgatt
     6361 gggtgatagt agagaatcct tgtttctacg gtgcgttgca ggcgctggag cggctacggc
     6421 tgaaggcgtt atcggtggcg acggatgtta aagaagggat cgatcttcag gcgctggaac
     6481 tggcgttgca ggagtatccg gtgaaagcgt gctggctgat gactaatagc cagaatccac
     6541 tcggatttac cttaacgccg caaaaaaaag cacaactggt ggcgttgctc aatcagtaca
     6601 acgtaacgct gattgaagat gacgtttaca gcgaacttta ttttggacgg gaaaaaccgc
     6661 tgcctgcgaa agcgtgggat cgccacgatg gcgttttgca ttgctcttcg ttttcgaaat
     6721 gtctggtgcc tggttttcgt attggttggg tcgccgccgg aaaacatgca cgtaaaattc
     6781 aacgcttgca gctgatgagt acgctttcca ccagctcacc gatgcagctt gcgctggtgg
     6841 attacctttc cacgcgccga tacgacgccc atcttcgtcg cctgcgtcgc cagcttgcgg
     6901 aacgtaaaca acgtgcctgg caggcactgc tgcgttatct gcctgcggaa gtgaaaattc
     6961 atcataatga cagcggttat tttctctggc tggagctccc cgagccgtta gatgccggcg
     7021 aattgagcct ggcggcactg acgcatcata tcagtattgc gccaggtaaa atgttttcta
     7081 ccggtgaaaa ctggtcacgt ttttttcgtt ttaataccgc gtggctgtgg ggagaacgtg
     7141 aagaacaggc ggtaaaacaa ttaggcaaac ttattcaaga acggctgtaa tagcgtttaa
     7201 tttaattcct cttagattgg gtaatatgaa tttcgaatag cagtcatatt tcctaactcc
     7261 ttgtctatac tccagaagat aaccttacag acggcataat gcgcggtagc tcacaacctg
     7321 attaaatttt ctcaggggcg aaggtgtgcc tgcaagccgc cgtctatggt taaacaagga
     7381 gatattttta cggcacggcg gctgaacaat taattacgac aggagtaaga ccttatgagc
     7441 aagacatttg cccgcagcag cctgtgtgcg ctcagcatga caataatgac cgctcacgcc
     7501 gccgaaccgc ctaccaattt agataaaccg gaagggcgac tggatattat cgcctggccg
     7561 ggatatatcg aacgcggaca aactgataaa caatacgact gggtaacgca attcgaaaaa
     7621 gagacaggct gcgcggtgaa tgttaaaacc gccgcgactt ccgatgagat ggtcagtctg
     7681 atgaccaaag ggggttacga tctggttacg gcatccggcg atgcctcgct gcgtttgatt
     7741 atgggtaaac gcgtgcagcc gattaatacc gccttgattc ccaactggaa aacgcttgat
     7801 ccgcgcgtgg ttaaaggcga ctggtttaac gttggcggca aagtttacgg cacaccttac
     7861 caatgggggc cgaacctgct gatgtacaac actaaaacct tcccgacgcc gccggatagc
     7921 tggcaagtgg tttttgttga gcaaaatctg caggacggca agagcaataa aggccgcgtt
     7981 caggcttatg atggccctat ctacattgcg gacgctgcgt tgttcgttaa agccactcag
     8041 ccgcagttgg gcatcagcga tccgtatcaa ctcaccgaag aacagtacca ggcggtgctg
     8101 aaagtgctgc gtgatcaaca tagtttgatc catcgctact ggcatgacac taccgtgcaa
     8161 atgagcgatt tcaaaaacga gggtgtggtc gcttccagtg cctggcccta tcagaccaac
     8221 gccctgaaag ccgaaggtca gcctgtcgct accgttttcc cgaaggaggg cgttaccggt
     8281 tgggctgaca ccaccatgct acatagcgaa gcgaaacatc cggtttgcgc ctacaaatgg
     8341 atgaactggt cattaacgcc aaaagtgcag ggcgatgtgg cggcctggtt tggctcgtta
     8401 ccagtcgtgc cggaagggtg taaagccagt tcgttattag gcgagaaagg ttgtgaaaca
     8461 aacggtttta actatttcga taaaatagcc ttctggaaaa cgcctatagc agaagggggc
     8521 aagtttgttc cctacagtcg ctggacgcag gattacattg ccattatggg cggtcgctaa
     8581 cttcgctggg gtgctttatg acgtacgcag tggagtttga caacgtctcg cggttgtacg
     8641 gtgacgtgcg cgcagtagat ggcgtcagta ttgcgataaa agatggtgag ttcttctcta
     8701 tgctggggcc gtccggctcc ggcaaaacca cctgcctgcg cctgattgct ggcttcgaac
     8761 agctttccgg cggggctatc tctatctttg gtaaacccgc cagcaatctg ccaccgtggg
     8821 agcgggacgt gaatactgtc tttcaggact acgcgctatt tccgcatatg tcgattcttg
     8881 acaatgtcgc ctatgggctg atggtcaaag gcgtgaataa aaagcagcgg cacgcaatgg
     8941 cgcaagaggc gctggagaaa gtggcgttgg ggtttgtaca gcaacgtaaa ccgtcacaac
     9001 tttctggtgg tcagcgccag cgggttgcta tcgccagagc attggtgaat gaaccgcgcg
     9061 tattgctgtt ggatgaaccg ctcggcgcac tggatctcaa attgcgtgag cagatgcagc
     9121 ttgagctgaa aaaactgcaa cagtctctcg gtatcacttt tatctgcgtt acccacgatc
     9181 agggcgaagc gttatcgatg tccgatcgtg tggcggtttt caataatgga cgcattgagc
     9241 aggtcgattc cccgcgcgat ctctatatgc gcccgcgcac gccgtttgtt gccgggttcg
     9301 ttggtacatc gaatgttttt gatggactga tggcagagaa actttgtggc atgacgggaa
     9361 gcttcgccct gcggccggaa catatccgcc tcaacacccc tggtgaactg caggccaatg
     9421 gcacgatcca ggcggtgcaa tatcagggcg cggcaactcg ttttgaactg aaattgaacg
     9481 gcggtgaaaa actgctggtg agtcaggcca atatgacagg cgaagaactg cctgccacgc
     9541 tcacgcccgg acaacaggtg atggtttcct ggtcgcgtga tgtgatggtg ccgctggttg
     9601 aggagaggtg aatggcgatg aacgtattgc aatcaccttc acgtccaggt ctgggtaagg
     9661 tgtccggttt cttctggcgg aatccggggc tggggctgtt tttgctgctg cttggcccgc
     9721 taatgtggtt tggcattgtc tatttcggct cgctgctaac gctgttatgg cagggctttt
     9781 atacctttga cgatttcacc atgtcggtaa cgccggaact gacgctggcg aacatccgtg
     9841 cgctgtttaa tccggcgaat tacgacatca ttctccgcac gctgactatg gctgtggcag
     9901 tcactatcgc cagcgccatt ctggcttttc caatggcgtg gtatatggcg cgctatacca
     9961 gcgggaaaat gaaagcgttt ttttatattg cggtaatgtt gccgatgtgg gcgagctaca
    10021 ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct cagtggtttt
    10081 tacaacatct tgggctggaa ccactgctga cagcgttcct tacattacct gcggtgggcg
    10141 gcaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat atctggctgc
    10201 cgttcatgat cctgcccgtg caggcggcgc ttgagcgttt gccgccgtca ttgttgcagg
    10261 cgtcagctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg ttgccgctgg
    10321 cgatcccggg tattgccgct ggctctatct ttaccttctc actcacactg ggcgatttta
    10381 tcgtcccgca gctggttggc ccgccaggat attttatcgg caatatggtt tattcccagc
    10441 agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg attgttctta
    10501 tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt tgatgcactc tgaacgcgca
    10561 ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt tcccatcctg
    10621 ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc accgcagggc
    10681 ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga tgccgtgaca
    10741 ctgtcactta aagtggcggc gctggcgaca ttaatggcgc tggtgttagg gacgctggca
    10801 gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct gttactgctg
    10861 ctgcccattg cgctgccggg cattgtcact ggtctggcgt tattaaccgc ctttaaaacc
    10921 atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt ttgtgtagtt
    10981 gtggtgttta acaatgtcat cgcccgtttt cgccgcacct catggagtct ggttgaggcg
    11041 tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt gccgaatctc
    11101 agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga tgaaatcatc
    11161 gttacgacct ttacggcagg tcatgaacga acgttaccgt tgtggttgct caatcagctt
    11221 gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat gttggtaaca
    11281 accttgccga tcctgggagc ctggtggcta acccgcgaag gcgacaatgg tcaataacca
    11341 ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact ggttagcggc
    11401 gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact ggagattgcc
    11461 gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc atttgccgaa
    11521 tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc tgatgttatc
    11581 gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa accgctgcat
    11641 agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt cgcgggtgcg
    11701 gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac ttcgatgatc
    11761 cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc gctgatgatg
    11821 gcagcgtgga aactggctcc ggcgctggcg gcagggaact gcgtagtgct taaaccatca
    11881 gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat cttcccggca
    11941 ggcgtgatta acgtactgtt tggcagaggc aaaacggtgg gcgatccgct gaccggtcat
    12001 cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca catcatcagc
    12061 cataccgcgc cctccattaa gcgtactcat atggaacttg gtggcaaagc gccagtgatt
    12121 gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt tggctattac
    12181 aatgccggac aggattgtac tgtggcttgt cggatctacg cgcaaaaagg catttacgat
    12241 acgctggtgg aaaaactcgg tgctgcagtg gcaacgttaa aatctggtgc gccggatgac
    12301 gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt cagcaaggca
    12361 gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga aaagcgcaag
    12421 ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga cgatgccatc
    12481 gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc tcttcgacaa cgaagaacag
    12541 gtggtaaact gggcgaatga cagccagtac ggacttgcat cttcggtatg gacgaaagat
    12601 gtgggcaggg cgcatcgcgt cagtgcacgg ctgcaatatg gttgtacctg ggtcaatacc
    12661 catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg ttacggcaag
    12721 gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat ggttaaacat
    12781 taaggataat attgcagatc gtaagagtat gagatgatct tgcgtactgt cagagcactg
    12841 atttctctga cagtacgtgc tgcgtgcaaa atcactacat caaataaacc agccaaatct
    12901 catagagata agagtaaaca aaccaacagc agcaaagaca tttatcagta ctacagttct
    12961 actggaaaca ttcatttttg ccacctttta tcattgcccc tatgtgtata gtacgggtta
    13021 agaaaatccg caataatact cattctgaag atggataagg gcaagttgct gtttgatgtt
    13081 tttttctcaa accggttaaa aaacttgatg cacgtcaaaa aatgacgcat atttgcgcgc
    13141 gttttattca tctggctgga cgcccgtaca tgtctctcta tcaacacatg cttgtttttt
    13201 atgcggttat ggcagcaatc gcatttctta tcacttggtt tctttctcac gataagaaac
    13261 gcatccgttt cttaagcgcc tttctggtgg gggcaacatg gccgatgagt ttcccggtgg
    13321 cgctgttgtt ttcactgttt taaatcttac tcactggctt gacggcacca cgcagactta
    13381 tatcatttgg atgaatcgat aaatttcaca agtggctaag gagaaagtat gtcgcatctg
    13441 gatgaagtca tcgcgcgcgt ggatgccgcg attgaagaga gcgtcattgc ccatatgaac
    13501 gaattattaa tcgccctgag cgatgacgcg gagttaagtc gggaagatcg ctacacccag
    13561 cagcagcgtt tgcgcaccgc tattgctcat cacggtcgca agcataaaga agatatggaa
    13621 gcgcgccatg aacagttaac caaaggcggc accatcctgt gattaaaatg agcgtctggc
    13681 taccaaccag gcgccaacca caagtaaaat tgcgccacaa attgggccga acaacgcccg
    13741 tagtggtacg ccatgactgc ggaagatatc cagaaccaga ccgccaatca gctggctggc
    13801 gaccagtact gcaatagttg tcgccgctcc cacattctga tatccgctga tactggcgaa
    13861 gacaaaaaac gatcccagca agccaggtat aagtgtccac cagcgcacgc tggacaccag
    13921 ttcgccaaac cccgccatac cttgtttaaa ccataaaata gaaacaaaga gcacaatccc
    13981 caccagtgag ttcagcaaca tggcgatgag aatggtagag gaggtctggg tgatgcgcac
    14041 catcagcgag ttctggacta ccagaccaat tccggcggca atcaaaaagg caagggtgag
    14101 cgactgattc atccaatcgc gtccggttca gtgcgttcgt cgagttgcaa ctgcataaat
    14161 gtcagatcca gccagcgccc aaatttagtg cccacctgcg gcatttgtgc ggtgacgaca
    14221 aatcccagcg actggtgcag atgcagcgag gcctgatttt gcgattcgat cccggcgacc
    14281 atgacatgct tcccgcaatc tcgcgcttca tcaatcaatc ggcttaacaa tttacgcccc
    14341 agacctttgc cctgatgatc gggatggaca taaaccgaat gttccacggt atggcgaaaa
    14401 ccatcgaaac tacgccagtc gccaaatgag gcatatcccg tcactacgcc atcttcttca
    14461 cttaccagca ccggataacc tgctatggtc cgcgcttcaa accaggcaat gcggttatct
    14521 gcatccaccg tttggtcatt ccagatagcc gccgtataca acacggcgtg gttataaatt
    14581 tccgcaatgg cagcacagtc ggctttgcgg gcaaaacgga tggacatgtc tggcctcaat
    14641 aaaataatga tgatgatgtt atcaatacta tcgaaaacag cccgaagcaa cggattcctc
    14701 gatgaaatta ttgcgtgttg cagagggggg gtgacggctg caaacaaaac caatcctgcg
    14761 agcctgctac tattgatgga ttcatgccaa aagcacagga gagcattatg gggcaacaaa
    14821 agcagcgtaa tcgacgttgg gttctggcct cgcgtccaca tggcgcaccg gttccggaga
    14881 atttccgtct tgaagaagat gatgtcgcca caccgggtga aggacaggtg ttactgcgca
    14941 cagtttattt gtccctggac ccgtatatgc gtggacgtat gagcgatgag ccatcttatt
    15001 caccgccggt tgatattggc ggcgtgatgg tcggcggtac ggtgagccgt gtcgtggagt
    15061 cgaatcatcc tgattatcag tctggcgact gggtgctggg ctacagtgga tggcaagact
    15121 atgacatatc cagtggtgat gatctggtga aacttggcga tcatccgcaa aatccatcgt
    15181 ggtcgctggg tgtgctaggg atgccaggct ttaccgctta tatgggccta ctggatatcg
    15241 gtcagcctaa agagggcgaa acgttggtgg tagctgcggc gacaggacca gtgggggcga
    15301 cggtggggca aatcggcaaa cttaaaggtt gcagagtggt gggggtagcc ggtggcgcgg
    15361 aaaaatgccg ccatgctacc gaggtgttag gcttcgatgt ttgtcttgat caccacgcgg
    15421 atgattttgc cgaacaactg gcgaaagcgt gcccaaaagg tattgatatc tattatgaaa
    15481 acgtgggcgg taaggtattc gatgcggtgc taccgttact taatacatct gcgcgcattc
    15541 ccgtctgcgg attagtgagc agctataacg ctacagagct accacccggt ccggatcgtt
    15601 tacctctgtt gatggctaca gtgctgaaaa aacgtattcg cttgcaaggt tttattatcg
    15661 ctcaggatta tggtcaccgc atccatgagt ttcagaggga gatggggcaa tgggtgaaag
    15721 aggataaaat ccactaccgc gaagaaatta ctgacggttt agagaatgcg ccacagacgt
    15781 ttatcggcct gctgaagggt aaaaacttcg gcaaagtggt gatccgcgtg gcgggtgatg
    15841 attaaagttt caacggcggc gtaagccgcc ataaaaatcc aggcaaaaag cttattttca
    15901 gctttaatta actaaccaca tcattgttct gctgctaata acaacaaagg tgagtggttt
    15961 atgattattc actttaatac accaggtgaa ttccttctgc catgcaggca gggttggaca
    16021 gaaaacacgc gaaggaatta aaatgcctgg aacggaaaaa atgaaacatg tcagtttgac
    16081 tctgcaggtt gagaacgacc tgaaacatca gcttagtatt ggcgcactaa aacctggcgc
    16141 acgcctgatt actaaaaatc tggcggagca attaggtatg agtattacac ctgtgcgtga
    16201 agcattatta cgtctggttt cggtgaatgc gctttctgtc gcaccagcgc aagcatttac
    16261 agttccggaa gtggggaaac gtcaattaga cgaaatcaat cggatccgct acgaactgga
    16321 attaatggca gttgctctgg ctgttgaaaa cctcaccccg caagacctgg cggaactcca
    16381 ggaattgctc gagaaattac agcaggcgca agaaaagggc gacatggaac aaatcattaa
    16441 tgtaaacagg ctatttcgct tagcgattta tcatcgctca aatatgccca tcctgtgtga
    16501 gatgattgag caactgtggg tcaggatggg acctggttta cattatctct atgaagcgat
    16561 taatccagcg gaattacggg agcatataga aaactatcat ctgttactcg ctgcgttaaa
    16621 agcaaaagac aaagagggat gcagacattg tcttgctgaa attatgcaac aaaatattgc
    16681 tattttatac cagcaataca atcgttaaag taaatgcccg gcaaataccg ggcatcaagg
    16741 taattactca aatcgccatg cgatattcac gccgacacca taatttcgcc cgggcgcagg
    16801 ttcgtaatat cgcccgtttg actcattgac aatgacagaa ccaacgtatt ctttatcgaa
    16861 taaattatcg acacgaccaa acaaatccac agttaaattg tggtaattgt atttataccc
    16921 ggtgaataag ccgacgagag tataagacgg cgctttggcc gtattttcat catctgccat
    16981 aatatcgccc atataacgcg cttccatgcc tgcataccaa ccatcttccg gtacataacc
    17041 tatcgacgca aagcccatat tacgggcgat ccctggcatc cgattaccgt tacaatcctg
    17101 ttcgttgcaa acattgctgc gataggtcgc atcaagccag gtccaggacg cgtttacgcg
    17161 aaaatctccc gcgaaacgtt gatcccatgc cagttcagcg ccttgacgac gggtctttcc
    17221 agcatttttg taagtcgtac gcccaccgct actgctatcg acaacaattt catcatcggt
    17281 gtcggtctgg aacaatgcga gactaagcag cccatcacca atacgcgttt tactgccgat
    17341 ctcaattgta tcgttggtgg atggttttaa accaaagttc ataccgcttt gcccatcagc
    17401 gcgataagac agctcattaa tcgtcggcgt ttcaaaacca cgcccggctg ccagatagat
    17461 attccaggca tcggtcattg catattttaa cgatccggct ggtagccatt tgtgataact
    17521 ggcatcaccg ctgtcatcgc cgttacccgg agtaacgtaa tggtcgttgg aatcaaacca
    17581 cacggagctg tagcgcacgc cagcatccag cgacagtttt tcgctcagct gccactgcgt
    17641 ctgtaaatag ggatcgatgt tccacatcag attgcgttcg tcgcgacgca actcaccttt
    17701 ttgcccgtac tccggcatgc cgctattcag gcggaagtta ttgtagccct tgcggttttc
    17761 actcatgttt tcgtagttca ggccggtagt gaacgtgacc ggaacgccca gttcaccacg
    17821 gtgtgtccag cggctgtcta ttccctggta atggcgttgc agggtaatca cgctgcccgc
    17881 atgtgacggg ttaagttgtg gtgccatggg tattgactgg tactgggtcg tttctcgctc
    17941 tccggcatac atcatcacac tcatatcatc ccgcgaactc aggctacgtt catagcgcaa
    18001 cccagcctga gtttgcttga tggtttttcg cgtgtcgtac tgttctgcac gaggcgcttg
    18061 ttgcggattc gctttccatt ctgctttggt tagcccacct gggtcatctg ctttgatatc
    18121 cacgctattg aaaatcaggc ttaatttgct ggcgtcatca atgcgtacgc ccagtttggc
    18181 attagctaaa tttttctgtg cgccactatg gtcacgatag ccgtgggtcg taaaacgcgt
    18241 ggttgagacg gtgtaatcaa catcgccagg ctgtgtgccg tctcccgttg cgcccgttgc
    18301 tttcagccca tagcgccagc tgccaaaact gccgtagtaa ctactggctt caatggttgg
    18361 tggctgttgc ccggtctggg tggtgacatt cattacaccg ccagacgcgt tgccatacag
    18421 ggcagagaag gggccacgca gcacttccac attttgcaca ctgcttaaat cgatgttgga
    18481 tgtttgccct tgcccgtcgg gcatggtagc gggaataccg tccacataca ggcgaatacc
    18541 gcgaattccg taagtggagc gggagccaaa tccacgaatc gacagctgta aatcttgcgc
    18601 atagttctgc cggttttgta cctgcaaacc aggcacgccg gtcagtgatt cggacaagtt
    18661 aatgcgcggt gttgccaggc gcatctcctc gccatccacc acgcttactg ctgcaggggt
    18721 atccagttct gaaaccacct gcggcgcggc actgacaatc atggtctgtt catcagcggc
    18781 aaaaacaacg ggggaaagga caagcagggc gggcaaaacg gtctgtcgaa cgaaaacaat
    18841 cttcatgaaa aaagccaggt taagaatggg aaaacaccgt catggtaatg aaattgtaaa
    18901 tttatggaaa acgaaacggc acgatacgtt aattaattga gagaattgta gtcgtaacgg
    18961 caagaaatgc tccacatttg agtaaataat gattaccatt cccatttata acaagagcgt
    19021 aacgatgatt acgcttagcg aagcattgtg aagcagcaaa aatatcggtt catcaaaggg
    19081 agtcgtcatg catttacgtc atctgttttc attgcgcctg cgtggttcat tactgttagg
    19141 ttcattgctt gttgcttcat cattcagtac gcaggccgca gaagaaatgc tgcgtaaagc
    19201 ggtaggtaaa ggtgcctacg aaatggctta tagccagcaa gaaaacgcgc tgtggctcgc
    19261 cacttcgcaa agccgcaaac tggataaagg cggcgtggtt tatcgtcttg atccggtcac
    19321 tctggaagtg acgcaggcga tccataacga tctcaagccg tttggtgcca ccatcaataa
    19381 cacgactcag acgttgtggt ttggtaacac cgtaaacagc gcggtcacgg cgatagatgc
    19441 caaaactggc gaggtgaaag gccgtctggt gctggatgat cgtaagcgca cggaagaggt
    19501 gcgcccgctg caaccgcgtg agctggtagc tgatgatgcc acgaacaccg tttacatcag
    19561 tggtattggt aaagatagcg tgatttgggt cgttgatggc gagaatatca aactgaaaac
    19621 cgccatccag aacaccggta aaatgagtac cggtctggca ctggatagca aaggcaaacg
    19681 tctttacacc actaacgctg acggcgaatt gattaccatc gacaccgccg acaataaaat
    19741 cctcagccgt aaaaagctgc tggatgacgg caaagagcac ttctttatca acatcagcct
    19801 tgataccgcc aggcagcgtg catttatcac cgattctaaa gcggcagaag tgttagtggt
    19861 cgatacccgt aatggcaata ttctggcgaa ggttgcggca ccggaatcac tggctgtgct
    19921 gtttaaccca gcgcgtaatg aagcctacgt aacgcatcgt caggcaggta aagtcagtgt
    19981 gattgacgcg aaaagctata aagtggtgaa aacgttcgat acgccgactc atccgaacag
    20041 cctggcgctg tctgccgatg gcaaaacgct gtatgtcagt gtgaaacaaa aatccactaa
    20101 acagcaggaa gctacccagc cagacgatgt gattcgtatt gcgctgtaat agatatggcg
    20161 agggtgataa cggagagact tgcctgactt tgatcacgcc gcatgcggca caccttcgtc
    20221 agtaatctaa atttgcccgg cacgtagccg ggcaattttg caattagctc gccgtttcag
    20281 gcttaaacac aatttcctgt ttttcttcat cttcctcgac gactggcgca gtgctgtgaa
    20341 tttcagcaac gcgtttgcgc acaccaaacc agcctataac cagcagaata ccgataatcg
    20401 gcagcgccgc gatagtataa gtcccgttcg ggtaatcgaa cgccatcagc acaaggacac
    20461 tcagtaaaaa cagtaatgtc agccaggaag tgaagggcgc gccaggcagt ttaaaactga
    20521 catccgctgc tttgccttct ttaatcgctt tacgcaggcg catctggcac acgatgataa
    20581 acgcccatga agcgatgatt cccagcgacg cgaagttcaa cacaatctca aatacgcgcg
    20641 acggcaccag atagttgagg aatacgccga cgacatacac aaccagtgtc gccagaatcc
    20701 cggcatacgg cacatgctga cgactcattt tcgccataaa actcggtgcg gaaccgccca
    20761 tcgccattga gcgcagaata cgtccggtgc agtacagacc tgaattcagg ctggagaggg
    20821 cagcggtcag caccacaatg ttcataatgc tgccgatata tggcacaccc agtttagaga
    20881 aaaacgtcac gaacggactt tgccccgcct gatacgcgct ccacggcaat aacataacca
    20941 gcaacaccac ggagccgacg taaaacaggc caatacgcca aatcacactg ttaatcgctt
    21001 taggcaccat ggtctgcgga tctttacatt ctccggcagc tgtacccacc atttcaatgg
    21061 aggcaaaagc aaacactacg ccctgaatca acaccagcgc aggcagcaaa ccgtggggga
    21121 agaagccgcc attatcggtg attaaatgaa agccagtggt gttgccatcc agcggctgac
    21181 cactaccgag gaacaccgta cccacgacca aaaaggtcac aatggcgagc actttaataa
    21241 gcgcaaacca gaactccatc tccgcaaacc atttcacacc gatcatattc atggtgccaa
    21301 cgatggtaag tgcagcgagc gcaaagaccc actgcggcac gccgccaaac gcaccccagt
    21361 aatgcatata cagagcgacg gcggtaatat caacaatccc cgtcatcgcc cagttgatga
    21421 agtacatcca gccagcaaca taagcggctt tctcacccaa aaactcacgg gcataagaga
    21481 caaaactgcc actggaaggg cggtgtagca ccagctcacc caatgcacgc agaataaaaa
    21541 acgaaaacaa gccacaaatt aaataaacca gtgccagtgc tggacccgcc atttgcagtc
    21601 gggctcctgc acctaaaaac aagccggtgc caatcgcgcc gccaatggcg atcatctgta
    21661 cctggcgatt gcccatcgct ttgtgatacc cctcttcgtg ggcattaagc cagcggcgtt
    21721 tcgcggcgtg ttgatctgaa gtgtcggtgt cgtgtttact cattgctctc cctgattgct
    21781 ttaatgaaaa agtcatataa gttgccatga acaatgttta ttcatcctgg cgatgatagt
    21841 tatcaccatt attctttatg ggtataaaac tatcggcgca gcatcctacc cgttatcgat
    21901 aaacgatgca aaacatcccc ttacaatcct gaaggggatt aatacaactg acgaaaaaat
    21961 gacaaatcct tttgctggtt aacctttgta ctgtcctaca tttaatcttt aaaagattgt
    22021 gaggggcata tgattaaggt gtatggcgta cccggatggg gctctacaat cagtgagctg
    22081 atgctaacac tggctgatat tccttatcag ttcgttgatg tcagtggttt tgaccacgag
    22141 ggaacttcac gcgagttatt gaaaaccctg aacccgttgt gtcaggttcc gacactggca
    22201 ctggaaaatg acgagattat gacggagaca gcggcgattg cattgatggt tctcgatcgt
    22261 cgcccggacc ttgcgccacc ggttgggcgt gccgaacgtc agcagtttca acggttattg
    22321 gtctggctgg ttgctaatgt ctatccaacg ttcactttcg ccgattaccc tgagcgttgg
    22381 gcatctgacg cacctgaaca gttaaagaag aatgttattg aatatcgtaa atcgctttat
    22441 gtctggctga attcacaatt gaccgctgaa ccttatgcgt ttggtgagca actaacgctg
    22501 gtggactgtt atctttgcac tatgcgcaca tgggggcctg ggcatgaatg gtttcaggat
    22561 aatgccacga atatcagtgc gattgctgat gcagtatgtc agttaccaac gttacaagaa
    22621 atactaaaaa ggaatgaaat tatttgagtg ggataccaga aga
//
LOCUS       DAZKFZ010000062        21877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000062 DAZKFZ010000000
VERSION     DAZKFZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            168..503
                     /locus_tag="ACHOQW_002166"
     CDS             168..503
                     /locus_tag="ACHOQW_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9573246.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            762..1205
                     /gene="yjdN"
                     /locus_tag="ACHOQW_002167"
     CDS             762..1205
                     /gene="yjdN"
                     /locus_tag="ACHOQW_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP9573247.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1338..2126
                     /gene="phnC"
                     /locus_tag="ACHOQW_002168"
     CDS             1338..2126
                     /gene="phnC"
                     /locus_tag="ACHOQW_002168"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9573248.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2151..3167
                     /gene="phnD"
                     /locus_tag="ACHOQW_002169"
     CDS             2151..3167
                     /gene="phnD"
                     /locus_tag="ACHOQW_002169"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9573249.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3273..4052
                     /gene="phnE"
                     /locus_tag="ACHOQW_002170"
     CDS             3273..4052
                     /gene="phnE"
                     /locus_tag="ACHOQW_002170"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9573250.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4073..4798
                     /gene="phnF"
                     /locus_tag="ACHOQW_002171"
     CDS             4073..4798
                     /gene="phnF"
                     /locus_tag="ACHOQW_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP9573251.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4799..5251
                     /gene="phnG"
                     /locus_tag="ACHOQW_002172"
     CDS             4799..5251
                     /gene="phnG"
                     /locus_tag="ACHOQW_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP9573252.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5248..5832
                     /gene="phnH"
                     /locus_tag="ACHOQW_002173"
     CDS             5248..5832
                     /gene="phnH"
                     /locus_tag="ACHOQW_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP9573253.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5832..6896
                     /gene="phnI"
                     /locus_tag="ACHOQW_002174"
     CDS             5832..6896
                     /gene="phnI"
                     /locus_tag="ACHOQW_002174"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP9573254.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6889..7734
                     /gene="phnJ"
                     /locus_tag="ACHOQW_002175"
     CDS             6889..7734
                     /gene="phnJ"
                     /locus_tag="ACHOQW_002175"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP9573255.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7731..8489
                     /gene="phnK"
                     /locus_tag="ACHOQW_002176"
     CDS             7731..8489
                     /gene="phnK"
                     /locus_tag="ACHOQW_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP9573256.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8600..9280
                     /gene="phnL"
                     /locus_tag="ACHOQW_002177"
     CDS             8600..9280
                     /gene="phnL"
                     /locus_tag="ACHOQW_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP9573257.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9277..10413
                     /gene="phnM"
                     /locus_tag="ACHOQW_002178"
     CDS             9277..10413
                     /gene="phnM"
                     /locus_tag="ACHOQW_002178"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP9573258.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10413..10970
                     /gene="phnN"
                     /locus_tag="ACHOQW_002179"
     CDS             10413..10970
                     /gene="phnN"
                     /locus_tag="ACHOQW_002179"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP9573259.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHHKEKHHACL"
     gene            10957..11392
                     /gene="phnO"
                     /locus_tag="ACHOQW_002180"
                     /pseudo
     CDS             10957..11392
                     /gene="phnO"
                     /locus_tag="ACHOQW_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11394..12164
                     /gene="phnP"
                     /locus_tag="ACHOQW_002181"
     CDS             11394..12164
                     /gene="phnP"
                     /locus_tag="ACHOQW_002181"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP9573260.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLHWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFEAGFDGMEIGVA"
     gene            complement(12241..12570)
                     /gene="yjdP"
                     /locus_tag="ACHOQW_002182"
     CDS             complement(12241..12570)
                     /gene="yjdP"
                     /locus_tag="ACHOQW_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP9573261.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12639..14909)
                     /locus_tag="ACHOQW_002183"
     CDS             complement(12639..14909)
                     /locus_tag="ACHOQW_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9573262.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15023..16543
                     /locus_tag="ACHOQW_002184"
     CDS             15023..16543
                     /locus_tag="ACHOQW_002184"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9573263.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16540..17514
                     /locus_tag="ACHOQW_002185"
     CDS             16540..17514
                     /locus_tag="ACHOQW_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9573264.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17533..18474
                     /locus_tag="ACHOQW_002186"
     CDS             17533..18474
                     /locus_tag="ACHOQW_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9573265.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18529..19212
                     /locus_tag="ACHOQW_002187"
     CDS             18529..19212
                     /locus_tag="ACHOQW_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFP9573266.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19227..20087
                     /locus_tag="ACHOQW_002188"
     CDS             19227..20087
                     /locus_tag="ACHOQW_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFP9573267.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20084..21085
                     /locus_tag="ACHOQW_002189"
     CDS             20084..21085
                     /locus_tag="ACHOQW_002189"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9573268.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21082..21789)
                     /locus_tag="ACHOQW_002190"
     CDS             complement(21082..21789)
                     /locus_tag="ACHOQW_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9573269.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
BASE COUNT         4770 a         6444 c         6200 g         4463 t
ORIGIN      
        1 gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga aaacgatcgt cgtctaaaat
       61 cagcagtacc cccgacaaac tcagggattt tgtgtataat tgcggccttt ttcggcaatc
      121 tgccgttttt tggcgctttt gccctgctga cttttgagga aatccacatg tcattaccac
      181 actgcccaaa atgcaactcc gaatacactt acgaagataa cggcatgtac atctgcccgg
      241 aatgtgccta cgaatggaac gacgcagaac ctgcacagga aagcgacgag ctgatcgtta
      301 aagatgctaa cggcaatctg ctggctgacg gcgacagcgt taccatcatt aaagatctga
      361 aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa agtgaaaaac atccgcctgg
      421 ttgaaggcga ccataacatc gattgcaaaa tcgacggttt tggtccgatg aaactgaaat
      481 ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta agccggataa ggcgtttacg
      541 ccgcatccgg caacagtgcc agatgcctga tgcgacgctc gcgcgtctta tcaggcctac
      601 aaattcccgc accctccgta ggccggataa ggcgtttacg ccgcatccgg caacagtgcc
      661 gactgcctga tgcgacgctc gcgcgtctta tcaggcttcc ctctcatctg tataaatttc
      721 gaactacact taactggctt ctcttaactg aggtcaccat catgccgtta agtccctacc
      781 tctcttttgc cggtaactgc tccgacgcga ttgcctatta tcaacgtacg ttgggcgctg
      841 aactgctcta taaaatcagc ttcggcgaaa tgccaaaatc agcgcaggac agcgccgaga
      901 actgcccttc cggaatgcaa tttcccgata ccgccatcgc ccacgccaat gtgcgcattg
      961 ccggaagcga tatcatgatg agcgatgcta taccgtcagg aaaagccagc tactccggtt
     1021 ttacgctggt gctcgattcg caacaggtcg aagaaggaaa acgctggttt gataaccttg
     1081 ccgctaacgg aaaaatcgaa atggcctggc aggaaacttt ctgggcgcat ggctttggca
     1141 aagtcaccga taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc
     1201 aataatccgc cgggaggccc gccctcccgc actgtcatcg aattcccgtt aactcttcat
     1261 ctgttagtca cttttaatta accaaatcgt cacaataatc cgccacgatg gagccacttt
     1321 tttagggagg ctgcatcatg caaacgatta tctgcgtcga acaactgagc aaaaccttca
     1381 accagcatca ggcgctgcac gcggttgatc tgaatatcca tcatggtgaa atggtggctc
     1441 tgcttgggcc gtccggttcc ggcaaatcca cccttttacg tcacttaagc ggtttgatta
     1501 ccggcgataa atccgccggt agccatatcg agctgctggg ccgcactgtc cagcgcgaag
     1561 gccgcctggc ccgcgatatc cgcaaaagcc gcgccaacac cggctacatc ttccaacaat
     1621 tcaacctggt gaatcgcctg agcgtactgg agaacgtgct gattggcgcg ctcggcagca
     1681 cgccgttctg gcgcacctgt tttagttggt tcacccgcga gcagaaacag cgcgcattac
     1741 aggcgctgac ccgcgtcggt atggcgcact ttgcgtatca gcgtgtttcc acgctctccg
     1801 gcggccagca gcagcgtgtg gcgattgccc gcgcgctgat gcagcaggcg aaagtgattc
     1861 tcgctgacga accgatcgcc tcgctggacc cggaatcggc gcgcatcgtg atggacaccc
     1921 tgcgcgacat caaccagaac gacggcatca ccgtggtcgt cacgctgcat caggtggatt
     1981 acgccctgcg ctactgcgaa cgcatcgtcg ccctgcgcca ggggcacgtc ttctacgacg
     2041 gcagcagcca acagtttgat aacgaacgtt ttgaccatct ctaccgcagc atcaaccgcg
     2101 tcgaagagaa cgcgaaagct gcctgacatc cccatcattg aggaaaacga atgaacgcta
     2161 agataattgc ctcgctggcc ttcaccagca tgttcagcct cagcaccctg ttaagcccgg
     2221 cacacgccga agagcaggaa aaggcgctga atttcggcat tatttcaacg gaatcacagc
     2281 aaaacctgaa accgcaatgg acgccgtttt tacaggatat ggagaagaag ctgggcgtga
     2341 aggtcaacgc cttctttgcc ccggactatg cgggcattat ccagggaatg cgtttcaaca
     2401 aagtggatat cgcctggtac ggcaacctgt cggcgatgga agcagtggat cgcgccaacg
     2461 gccaagtctt cgctcagacc gtcgcggcgg atggatcgcc gggttactgg agcgtgttga
     2521 tcgtcaacaa agacagcccg atcaacaacc tgaacgatct gctggcgaag cggaaagatc
     2581 tcaccttcgg taatggcgat cctaactcca cctctggctt cctcgtcccc ggctactacg
     2641 tcttctccaa aaacaatatc tccgccagtg acttcaagcg caccgtcaac gccgggcatg
     2701 aaaccaacgc gctggccgtc gccaacaagc aggtggatgt ggcgaccaac aacaccgaaa
     2761 acctcgacaa gctgaaaacc tccgcgccgg agaagctgaa agaactgaaa gtgatctgga
     2821 agtcaccgct gatcccaggc gatccgatcg tctggcgtaa aaacctttcc gaaaccacca
     2881 aagacaagat ctacgacttc tttatgaatt acggcaagac gccggaagag aaagcggtgc
     2941 tggaacgcct gggctgggcg ccgttccgcg cctccagcga cctgcaactg gtgccgattc
     3001 gccagctcgc actgtttaaa gagatgcaaa gcgtgaaagg caataaaggg ctgaatgagc
     3061 aggacaagct ggcaaaaacc accgagattc aagcgcagct ggatgacctg gaccgcctga
     3121 acaacgcgtt aagcgcgatg agttcggtaa gtaaagcgat gcagtaaatc gtaggtcgga
     3181 taagacgccc cggtgtcgca tccgacaatg tgcaggcgtt gatgccggat gcggtgcaag
     3241 caccttatcc ggcctacaga ccggagccaa acatgcaaac catcaccatc gccccaccca
     3301 agcgcagctg gttctcgctt ctgagctggg ccgttgtact cgccgtgttg gtcgtctcgt
     3361 ggcagggcgc ggaaatggcc ccgctcacgc tgatcaaaga cggcggcaac atggcgacct
     3421 tcgccgccga cttcttcccg cccgatttca gccagtggca ggattacctc accgaaatgc
     3481 tcgtcacgct gcaaatcgcc gtctggggca ccgcgctggc ggtggttctc tccatcccct
     3541 tcggcctgat gagcgccgaa aacctggtgc cgtggtgggt ttaccagccc gtgcgccgcc
     3601 tgatggacgc ctgccgcgcc attaacgaaa tggtcttcgc catgctgttc gtggtcgccg
     3661 tcggcctcgg cccattcgct ggtgtgctgg cgctgtttat ccacaccacc ggtgtgctct
     3721 ccaaactgct ttccgaagcg gtggaagcga ttgaacccgg cccggtggaa ggcattcgcg
     3781 ccaccggcgc caacaagctc gaagagatcc tctacggcgt gctgccgcag gtgatgccgc
     3841 tgctgatctc ctactccctc tatcgcttcg aatccaacgt ccgctcggcg accgtcgtcg
     3901 gcatggtcgg cgcgggcggg atcggcgtca cgctgtggga agcgattcgc ggtttccagt
     3961 tccagcaaac ctgcgccctg atggtgctga ttatcgtcac ggtcagcctg ctggatttcc
     4021 tctctcaacg gttgcgtaag cactttatct gattagcgag gcattgatag ctatgcactt
     4081 gtctacacat ccgaccagct acccaacacg ctatcaggag atagccgcaa aacttgagca
     4141 ggagcttcgt caacactacc gctgcggcga ctatcttccc gccgagcagc agctggcggc
     4201 gcgctttgag gtaaatcgcc acaccctgcg ccgcgccatc gaccaactgg tggaaaaagg
     4261 ctgggtacag cgccgtcagg gcgtcggcgt gctggtgctg atgcgcccgt tcgattaccc
     4321 gctcaacgcc caggcgcgtt ttagccagaa tctgctggat cagggcagcc atcccaccag
     4381 cgaaaaactg ctgtctgtgc tgcgcccggc ctccggtcac gtcgctgacg cgctggggat
     4441 aaccgagggg gagaacgtca tccatctgcg caccctgcgc cgggtcaacg gcatcgcgct
     4501 ctgtttaatc gaccactact tcgcggacct caccctctgg ccgacgctgc aacgcttcga
     4561 cagcggctcg ctgcacgatt ttctgcgcga gcaaacgggg attgcactgc gccgcagcca
     4621 gacgcggatc agcgcccgcc gcgcccaggc caaagagtgc cagcgtcttg aaattccgaa
     4681 tatgtccccg ctgctgtgcg tgcgcaccct taaccaccgt gacggtgaaa gcagcccggc
     4741 ggagtactcc gtcagcctga cgcgcgccga catgattgaa ttcactatgg agcactgaat
     4801 gcacgcagat accgcgaccc gccagcactg gatgtccgta ctggcacaca gccaaccggc
     4861 agaactggca gcccgcctga acgcgctgaa catcaccgcc aactatgagg tgatccgcgc
     4921 cgctgaaact ggcctggtac agattcaggc gcggatgggc ggcaccggcg aacgcttttt
     4981 tgccggcgac gccacgctga cccgcgccgc cgtacgtctg actgacggca ccctcggcta
     5041 cagctgggtg ctggggcgcg acaaacagca cgccgaacgc tgcgcgctga ttgacgcgct
     5101 gatgcagcaa acccgccatt ttcaaaactt atcagaaacg cttattgccc cgctggacgc
     5161 tgaccgtatg gcacgcattg ccgcacgcca ggccgaagtg aacgccagcc gggtcgactt
     5221 ctttacgatg gttcgcggag acaacgcatg accctggaaa ccgcttttat gcttcccgtg
     5281 caggatgccc agcacagttt tcgtcgcctg ttaaaggcca tgagcgagcc gggcgtgatt
     5341 gtcgccctgc atcagctcaa acgcggctgg cagccgctga atatcgccac caccagcgtg
     5401 ctgctgacgc tggccgataa cgacacgccg gtgtggcttg ccgcgccatt gagcaacgat
     5461 atcgtcagcc agagcctgcg ttttcatacc aacgcgccgc tggtcagcca gccggaacag
     5521 gcgaccttcg cggtaacgga tgaggcgatt tccagcgaac agcttaacgc cctttccacc
     5581 ggcaccgccg ttgcgccgga agcgggcgca acgctgattt tacaggtcgc cagcctgagc
     5641 ggcgggcgca tgctgcgtct caccggcgcg ggtattgccg aagaacgaat gatcgccccg
     5701 cagctgccgg agtgcattct gcacgaactc accgagcgcc cgcacccgtt cccgctcggc
     5761 atcgacctga tcctgacctg cggcgagcgc ctgctggctg ttccgcgaac cactcatgtg
     5821 gaggtgtgct gatgtacgtt gccgtgaaag ggggcgagaa ggcgatcgac gccgcccacg
     5881 ccctgcaaga gagccgacgc cggggcgata ccgatttgcc tgaactgagc gtcgcccaga
     5941 ttgaacagca gcttaatctt gcggtggatc gcgtgatgac cgaaggcggc attgccgacc
     6001 gcgagctggc ggcgctggcg ctgaaacagg ccagcggcga taacgtcgaa gcaattttcc
     6061 tgctgcgcgc ctaccgcacc acgctggcga agctcgcggt aagcgagccg ctcgacacca
     6121 cggagatgcg tctcgaacgg cgtatttcag cggtttataa agatattccc ggcggccagc
     6181 tgcttggccc aacctacgac tacacccatc gcctgctcga tttcaccctg ctggcaaacg
     6241 gcgaagcgcc gacgctgacc accgccgaca gcgaacagca gccgtcgccg cacgttttca
     6301 gcctgctggc gcgtcagggg ctggcgaagt ttgaagagga tagcggcgca cagccggatg
     6361 acatcacccg cacgccaccg gtttacccct gctcacgttc ttcccgcctg caacagctca
     6421 tgcgcggcga cgaaggctat ttgctggcgc tggcctactc cacccaacgc ggttacgggc
     6481 gcaatcaccc gttcgcgggt gaaatccgca gtggttacat cgacgtgtcg attgtgcccg
     6541 aagagctggg atttgcggtg aacgtcggcg aactgctgat gactgagtgt gaaatggtca
     6601 acggttttat cgacccaccg gatgaatctc cgcacttcac gcgcggctac gggctggtgt
     6661 tcggcatgag cgagcgcaaa gcgatggcga tggcgctggt cgaccgcgct ctgcaagccc
     6721 cggagtacgg cgagcacgcg acaggcccgg cgcaggatga agagttcgtg ctggcacatg
     6781 ccgacaacgt cgaagccgca ggctttgtct cgcacctcaa actcccgcac tacgtcgatt
     6841 tccaggccga actggagcta ctcaaacgtc tgcaacagga gcagaaccat ggctaatctg
     6901 agcggctaca actttgccta cctcgacgag cagaccaaac gcatgatccg ccgcgccatc
     6961 ttaaaagcgg tggcgatccc cggttatcag gtgccgtttg gcgggcgcga gatgccaatg
     7021 ccgtacggct ggggaaccgg cggcattcag cttaccgcca gcgtgattgg cgaaagcgac
     7081 gtgctgaagg tgattgacca gggcgcggat gacaccacca acgccgtgtc gattcgcaac
     7141 ttctttaagc gcgtgaccgg ggtaaacacc accgagcgca cggacgatgc aacggtcatc
     7201 cagacgcgtc accgcatccc cgaaacgccg ctgaccgaag atcagatcat tattttccag
     7261 gtgccgatcc ccgaaccgct gcgctttatc gagccgcgcg aaacggaaac ccgcaccatg
     7321 cacgcgctgg aggaatacgg cgtgatgcag gtgaagctgt atgaagatat cgcccgcttc
     7381 ggtcatatcg ccaccaccta cgcctatccg gtgaaggtga acgggcgcta cgtaatggac
     7441 ccgtcgccga tcccgaaatt cgataacccg aaaatggaca tgatgcccgc cctgcaactc
     7501 ttcggcgccg ggcgtgagaa gcgcatctat gcggtgccgc cgtttacccg cgtggaaagc
     7561 ctcgatttcg acgatcaccc gttcaccgtt cagcagtggg atgagccatg cgccatctgc
     7621 ggatcgaccc acagctatct tgatgaagtg gtgctggatg acgccggaaa ccgcatgttt
     7681 gtctgctccg ataccgatta ttgccgccaa cagagcgagg caaaaagcca atgaatcaac
     7741 cgttactttc ggtcaataac ctgacccacc tttacgcgcc gggcaaaggc tttagcgatg
     7801 tctcttttga tttatggccg ggggaagtgc tgggcattgt cggggaatcc ggctccggga
     7861 agaccacgct gctgaaatca atttccgcgc gtctgacgcc gcagcagggg gagattcact
     7921 atgaaaaccg ttccctctat ggcatgagcg aggccgaccg ccgtcgcctg ctgcgcaccg
     7981 aatggggcgt agtgcatcag catccgctcg acggcctgcg ccgccaggtc tcggcaggcg
     8041 gcaatatcgg cgagcggctg atggcgaccg gggcgcgtca ttacggcgat attcgcgcca
     8101 ccgcgcagaa gtggctggaa gaggtggaaa ttcccgccaa ccggatcgac gacctgccga
     8161 ccaccttttc cggcggtatg cagcagcgtt tgcagattgc ccgcaacctg gtgacgcatc
     8221 cgaagctggt gtttatggat gaaccgaccg gcgggctgga tgtgtcggtg caggcccgcc
     8281 tgctcgacct gctgcgcggc ctggtggtgg agctgaacct cgcggtagtg attgtcaccc
     8341 acgatttagg cgtcgcccgc ctgctggcgg accgtttgct ggtgatgaag caggggcaag
     8401 tggtggagag tgggttaacc gaccgcgtgc tcgacgaccc gcatcatccg tatacacagc
     8461 tgctggtgtc atcggttttg cagaattgag ccggtgccgg atgcggcgta aacgccttat
     8521 ccggcctaca aatgcgctcc ccgtaggtcg gataagacgc atcagcgtcg catccgacac
     8581 ccgaaccacg aggcgaaaaa tgattaacgt acaaaacgtc agtaaaacct tcatcctgca
     8641 ccagcaaaac ggcgtgcgct tgcccgtcct caaccgcgcc tcgctcaccg tcaacgcggg
     8701 cgaatgcgtg gtgctccacg gccactccgg cagcggcaaa tcaaccctgc tgcgctcgct
     8761 gtacgccaac tatctgcccg atgaagggca aatccagatc aaacacggtg acgagtgggt
     8821 agacctggtc actgcgccag cgcgcaaagt ggtggaaatc cgcaaaacca ccgttggctg
     8881 ggtgagccag tttctgcgcg ttatcccgcg catttcggcg ctggaagtag taatgcagcc
     8941 gctgctcgat accggcgtac cgcgtgaagc ctgcgccgct aaagccgcgc gtctgctcac
     9001 ccgtctgaac gtgccggaac gcctgtggcg ccttgcgcca tcgaccttct ccggcggcga
     9061 acagcagcgc gtcaacatcg cccgtggctt tatcgtcgac taccccattc tgctgctcga
     9121 cgaacctacc gcctcgctgg acgccaaaaa cagcgccgcc gtagtcgaac tgatccgaga
     9181 agccaaagcc cgcggcgcag ccatcgtcgg cattttccat gacgaagccg tacgtaatga
     9241 cgtcgccgac cgcctgcacc caatgggagc ctcttcatga ttatcaataa cgtcaaactg
     9301 gtgctggaaa acgaagtggt aaacggttcg ctggagatgc aggacggcga aatccgcgcc
     9361 tttgccgaaa gccagagccg cctgccggag gcgatggacg gcgaaggcgg ctggctgctg
     9421 ccggggctga ttgagctgca tacagacaac ctcgataaat tcttcacccc gcgcccgaaa
     9481 gttgactggc ccgcccactc ggcgatgagc agccatgacg cgctgatggt ggcgagcggc
     9541 atcaccaccg tactggacgc cgtggcgatt ggcgacgtgc gagacggcgg cgatcggctg
     9601 gagaatctgg agaagatgat caacgccatc gaagagacgc agaaacgcgg cgtcaaccgc
     9661 gccgagcacc gcctgcacct gcgctgcgag ctgccgcata acaccacgct gccgctgttt
     9721 gaaaagctgg tgcagcgcga gccggtgacg ctggtgtcgc tgatggatca ctcgccgggc
     9781 cagcgccagt tcgccaaccg cgagaagtat cgcgaatatt atcagggcaa atactcgctc
     9841 accgatgcgc agatgcagca gtatgaagag gaacagctgg cgcttgccgc acgctggtcg
     9901 cagccgaatc gcgaatccat cgccgcaatg tgccgcgcgc gacaaattgc gctcgccagc
     9961 cacgatgacg caacgcacgc ccacgttgcc gaatctcacc agcttggcag cgtgatcgcc
    10021 gaatttccca ccacgtttga agcagcggaa gcctcgcgca agcatggcat gaacgtgctg
    10081 atgggcgcgc caaatatcgt gcgtggcggt tcgcactccg gcaacgtggc ggccagtgaa
    10141 ctggcacagc ttggcctgct ggatatcctc tcttccgact actaccccgc cagcctgctg
    10201 gatgcggcgt tccgcgtcgc cgatgaccag agcaaccgct ttacgctgcc gcaggcggtg
    10261 aagctggtga ccaaaaatcc ggcgcaggcg cttaatctcc aggatcgcgg ggtgattggc
    10321 gagggcaaac gcgccgacct ggtgctggcg catcgcaagg gcaatcacat tcatatcgac
    10381 cacgtctggc gtcagggtaa aagggtgttc tgatgatggg aaaactgatt tggttaatgg
    10441 ggccgtccgg ctccgggaaa gatagcctgc tggcggaact ccgcctgcgg gaacaaactc
    10501 agctactggt ggcgcatcgc tacatcacgc gcgacgccag cgccggaagc gaaaaccata
    10561 tcgccctgag cgagcaggaa ttttttaccc gcgcggggca aaatctgctg gccttaagct
    10621 ggcacgctaa cggtctgtat tatggcgtcg gcgtcgagat tgacctctgg ctgcacgctg
    10681 gattcgacgt gctggtcaac ggctcacgcg cccatctgcc gcaggcgcgg gcgcgctatc
    10741 aatcggcgct gctgcccatt tgcttacagg tttcgccaga gatcctgcgc cagcgcctgg
    10801 aaaaccgtgg ccgcgaaaac gccagtgaaa ttaacgcccg gctggcgcgc gccgcccgct
    10861 atactccaca ggattgccat acgctcaata atgacggcag cctgcgccag tcggtcgaca
    10921 cgctgctgac gctaatccat cataaggaga aacaccatgc ctgcttgtga gctttcgccc
    10981 ggccacgcag tacgacaccg acgcggttta cgcgttgatt tgcgagctaa aacaggcgga
    11041 gtttgaccac cacgcgtttc gcgtgggctt taacgccaac ctgcgcgacc cgaacatgcg
    11101 ctaccatctg gcgctgctgg atggcgaagt tgtcggtatg atcggcctgc atttgcagtt
    11161 tcatctgcat catgtcaact ggatcggcga aattcaggag ttggtggtaa tgccgcaggc
    11221 gcgcggtctg aacgtcggca gtaagttact ggcgtgggca gaagaagaag cccgccaggc
    11281 cggggccgaa atgaccgaac tttcgaccaa cgtgaagcgc cacgacgcgc accgtttcta
    11341 tctgcgcgaa ggctacgagc agagccactt ccgcttcacc aaggtgctgt aacatgagcc
    11401 tgaccctcac gctcaccggc tccggcggcg cacagggcgt tccggcatgg ggctgcgagt
    11461 gtgcggcctg cgccagagcg cggcgctcgc cgcactatcg ccgccagcca tgcagcggcg
    11521 tagtgaagtt taacgaggcg atcaccctga tcgacgccgg gcggcacgat ctcgccgatc
    11581 gctggtcgcc cggatcgttc cagcagtttt tgctgacgca ctaccatatg gatcacgtgc
    11641 aggggctatt cccgctgcac tggggcgtgg gcgatccgat cccggtttac ggcccaccgg
    11701 atgaacaggg ctgcgacgat ctgtttaaac atccgggcct gcttgatttc agccacacgg
    11761 tggaaccgtt tgtggtgttt gatttgcagg ggttacaggt cacacccctg ccgctcaacc
    11821 actcaaaact gaccttcggt tatctgctgg aaacggcgca cagccgggtg gcgtggctgt
    11881 ctgacaccgc tggcttgccg gaaaaaacgc tgaaattttt acgcaataat cagccgcagg
    11941 taatggtgat tgattgcagc cacccgccgc gcgaggatgc accgcgtaat cactgtgatt
    12001 taaataccgt gcttgcgctg aatcaggtta tccgctcgcc gcgcgtggtt ctgacccata
    12061 tcagccacca gtttgatgcg tggctgatgg aaaacgcact gccgtcgggg tttgaggcgg
    12121 ggtttgaggc ggggtttgat gggatggaga ttggggtggc gtgatgagag ggaatgtgcg
    12181 tgctggcccc ctcaccctaa ccctctcccc agaggggcga tgcctgcccc atacccaaca
    12241 tcattgccca tactcatctt ccattctccg ctcttcatct tctagctgcc gacgctcctg
    12301 attaagctgg tgctggcgat cgtccagctg cctgcgacga tcttcaaact ggcgacggcg
    12361 gtcgtcatat tgtctgcgcc gatcgtcgct cacttcacgc tgccagccgt cgtcgcgcga
    12421 atcttcataa tctcgcccac ggtcagggtt agaagcgtcg ttaatcgcct gctgaatatt
    12481 gccaatggtg tcatcgataa tatcggcctg ggccggtacg gtggtcagcg tgagcagggt
    12541 gaaaagaaaa agcgtggagt ggcgtttcat aaactcggcc tcgcaacaaa gttaatacaa
    12601 ggtaggcaaa ggtgcctgtt acgagtagag gaggattctc aattctcttg ccccagcgcc
    12661 tgcgccaact gcgcacgggt aaagggtttg cgcaacagcg ccacgtctgg cagcgcgggg
    12721 ttatggctgg ggcgtaaatc ctgaccgctg atgagtagca gcgtcaggtg cgggtaaagt
    12781 ttgcgggccg cattgaccac ttctgcgccg ctcatgccgc cggggagcat taaatcgctg
    12841 ataaaaatat cgatttctgc tgatgccgcc agcagatcca gcgcctgttc gccgctgctt
    12901 gcttccagcg tcaaatagcc cagcaggtgc aactgttcgc agatggtctg gcgcaccgcg
    12961 gcttcatctt ccagcactaa caccagttta tcgccgctga cagaagcctg ttctactgcc
    13021 gctggcgata ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc
    13081 ccctgccccg gcgcgctttc aatttcaacg cgcccaccgg actggcgcac aaaaccatag
    13141 accatcgaca aacccagccc gctgccgctg cccgtctgtt tggtggtaaa gaatggctca
    13201 aatacctggc ttttcaccgc ctgcgacata ccgcagccat gatcgatgac ctccagtgcc
    13261 accatgtcct gacgctgccc actgctgcga gtaacgcgct gattccaggt acgaattttg
    13321 atgattcccg tctgcccttc catcgcgtca cgggcgttca tcaccaggtt gatgatggca
    13381 ttttccagtt ggctgatgtc aatccacgcg ggccacgccg gagactgcgc ttcaatctcc
    13441 agcgccagcg ttgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcaatgtt
    13501 ttcagttcaa cggcttgcgg atgtaaggac tgcttgcggg aaaacgccag cagtcgttgt
    13561 gtcagtagcg ccccacgttc ggcagcttgc agcgcccgct gtattcgcgg cgcgtctggc
    13621 gagtccggat tcaccagctc caggctgcca ataatcaccg ccagcagatt attaaaatca
    13681 tgggccagcc cgcccgtcag ttgcccgaca gctttcagtt tctggctgtg taatagcgcc
    13741 tcttccaacc cctgccgctc agtttgggca atctccatct gcgtggtctt ctcttttagc
    13801 agccgtgagg ttcgttcgag tgacgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc
    13861 agttcaccca actcatcacg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc
    13921 gccaggcggc tcatggcgcg ggaaatcgcc gtcaggttag tgccgaggtt gcgatagata
    13981 taccagccgg caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcaccctc
    14041 tcgccggagc gcaaatcctg gtaactttgc gccacacgct gaccggagag cgtcgccacc
    14101 tgttcggcgt aacggttgat gtcgccattg aggatcgcca ccagcgcttt gatgtgaaac
    14161 atataccagc tgattgccag atcgctttgc tccagagcgg cagaaagcgg cgcgagttta
    14221 cgcatttcat cattgaaatc attgaaatca ttgagtaacg tattaaccaa tggcgtatcg
    14281 ctgtgggtcg gcaacgcaga cattaccccg accagttgat gaataatcgc ctgcggcgcg
    14341 ggggtggcaa tagcggcaac aatcagtcgg ttcatctcgt taagcaaaat atcatcttgc
    14401 gcgtgagtga ctttttgcag atgccgcaga taacttaagt tttgatacag cgaacttaac
    14461 agcgcgttgc gctccagatg ccgcctttgc ccgcgctcca gcataccgcc gacgctggtt
    14521 tgcagttcgt tgctacgcgt gatgattcgc gtcaccagcc cggcttcctg ctgcgctaaa
    14581 ggcgcgttcg ccagttgtgc aagcgagctt tttaatgccc gctgtgtctc ctgcaaccgc
    14641 tcggcttcgc ctttatattc cagcgctccc accacctgcg acaatcgcac cgccgccgtt
    14701 gccacgtttg ccgtgtcgcg cgccagattc aggctgccgg tcatatcatc caccgtttgc
    14761 tgctgcacca gctcctgcat ctggctggca tgacgaaagc ccattaccgc cacgccgctg
    14821 accatcaacg tcaccgccac caccagcaaa ttcaaaagca gcaatcgccc gcgcgcgctg
    14881 gcaaaaaagt gacgtccggg gtgcgccata tcacgctccg tgaagaaaaa tcgcaatatg
    14941 acaaatatga acggcttctg acattttgca tttactttcc tgtgatggag tgctgatatc
    15001 gacaacctcg caggagtcca ccatgaacag cacgccagtt ctggagatgc gcaatattgc
    15061 caaagccttc ggcaaatttt atgcactcaa aggggtggat ttgacggtct accctggcga
    15121 gatccatgcc ctgatggggg aaaacggcgc gggaaaaagc acgctgatga aggtgctggc
    15181 gggggcgtat accgccacca gcggcgagat tctcatagac ggcaagccct ttcacattcg
    15241 cacgccaaaa gatgccttaa gcgccggtat tacgctgatt tatcaggaga tgcagctggc
    15301 accgaatctg tcggtggcag aaaatatttt tctcggcagc gagctttccc acggcgggct
    15361 ggtgcagcgt aaagagatgc tagtgcaggc gcaaaaagtg atcgaccgcc tcggcgcgca
    15421 gtttaacgcc agcgataagg tcatgacgct gaccattgcc gagcaacagc aggtcgaaat
    15481 cgcccgcgca ctacatcgca acagccgcat tctggtgatg gacgaaccca ccgctgccct
    15541 ctcctcccgc gaaactcacc gcctgtttga actgattatg cggctgcgcg atgaagggat
    15601 ggcgattatc tacattagcc accgcatggc ggaagtgtat gagctttccg atcgcgtcag
    15661 cgtgctacgc gacgggcaat acgttggcag cctgacccgc gataacctca atgccgggga
    15721 gctggtgcgg atgatggtcg gcaggccact gagcgatctg ttcaataaag agcgcgatat
    15781 cccgctcggt aaagcccgcc tgaatgttca ccacctgacc gacggcggca aagtccagcc
    15841 gagtagcctg ctggtgcgtt ccggcgaaat tgttggcctc gccggactgg tgggtgccgg
    15901 acgttccgaa ctggcgcagt tgatcttcgg cgtgcggaaa gcgacaggcg gaatgattga
    15961 agtcgatggt gaaccggtgg tgatccactc cccgcgcgaa gccatcgatc ttggcattgg
    16021 ttttctcacc gaaaaccgca aagaacaagg cttattcctt gaaatggcag cagccgaaaa
    16081 catcaccatg gcaaccctgg agcgcgatgc ccgctgggga atgctcaatc gcaaaaaagc
    16141 gcaaaccatt tccgatgacg ccattaagtt gctcaacatt cgcgtgcctc atgcccaggt
    16201 acgcgcgggc gggctttccg gcggcaatca gcaaaaactg ttgatctccc gctgggtggc
    16261 gattggcccg cgcattttac tgctcgatga acccgcccgc ggcgtggacg ttggcgccaa
    16321 aagtgagatc taccggatca tgaacgagat ggcgcgcaag ggcgtggcga tcctgatgat
    16381 ctccagcgaa ctgccggaga tagtgggaat gagcgatcgc gtctatgtga tgcgcgaagg
    16441 cagcattgcc ggtgagttaa acggcaaaaa catcacccag gaaaacatta tgaccttagc
    16501 gactggcgtc aacgacgccc attcccaggc ggtaacctca tgacgacctc tgcaaaaacc
    16561 tctgccaaaa aaatgctgat gagcgatctg atgcaaacgg tcggcatctt gcccattttg
    16621 attctgatcg tggcggtatt tggctttatc acgccaaact tttttaccga aagcaacctg
    16681 ctgaatatta cccgccaggc atcgatcaac attgttctgg cggcggggat gaccttcatc
    16741 attttgaccg gcgggatcga tctttccgtg ggttcgatcc tcggcaccac agcggtggcg
    16801 gcgatggtcg tctcccttag ccctgagttc gccatgctgt cgatcccggc agcgctgatg
    16861 ctcggcctgc tgctggggct gttcaacggc gcgctggtgg cctttgccgg attgccaccg
    16921 tttatcgtta cgcttggcac ctatacggcg ctgcgcgggg cggcgtatct gctggcggac
    16981 ggcaccacgg ttatcaactc caacattaat ttcgagtgga taggcaacaa ctatctcggg
    17041 ccggtcccat ggctggtggt gattgcgctg gcggtgatcg tggtgtgctg gtttattctg
    17101 cgccgcacca cgcttggcgt tcatatctac gccgtcggcg gcaatatgca ggcggcgcgt
    17161 cttaccggca ttaaggtctg gctggtgctg ctgtttgttt atggcatgag cggcctgctt
    17221 tctgggcttg gtgggattat gagcgcctca cggctgtaca gcgccaacgg caacttaggc
    17281 atgggttacg aactggatgc cattgcagcg gtgatcctcg gcggcaccag ttttgtcggc
    17341 ggcatcggca ccatcaccgg cacgctggtc ggcgcgctaa ttatcgccac gctcaacaat
    17401 ggcatgacct taatgggcgt ctcttacttc tggcaactgg tgatcaaagg ggcgatgatc
    17461 atcattgcgg tgctgatcga caaataccgt acccgacacc atcaaagtgc ataaacacat
    17521 gaggaataca gcatgagatt gaagccctta gtgaccgcgc tctgtgcagg cgcgctgctg
    17581 gccgcagcac cgttcgccca ggcgaaagaa ctgaaatcaa taggcgtaac ggtgggcgat
    17641 ctggcgaacc cgttcttcgt gcagatcacc aaaggcgcgg agctggaagc acgcaaactg
    17701 gcaggcgata acgtcaaagt gacgctggtt tccagcggct acgatctggg tcagcaggtg
    17761 tcgcagatag acaactttat cgccgctaac gtcgatatga ttatcctcaa cgctgccgat
    17821 tcaaaaggga tcggcccggc ggtgaaacgg gcgaaagatg ccgggatcgt ggtcgtcgcg
    17881 gttgacgtag cggcagaagg tgccgatgcc accatcactt ccgataacac ccaggcaggc
    17941 gaaatggcct gtaaatacat caccgatcgc ctgaaaggca aaggcaacgt ggtgatcatc
    18001 aacggcccgc cggtatccgc cgtgcaaaac cgcgtcgaag gatgccagac tgaatttaaa
    18061 cgccatcctg acatcaaagt gctctctgac aaccagaacg cgaaaggcag tcgtgaaggc
    18121 gggctggagg taatgacggg actgctggcg gcaaatccga agatcgacgg cgtatttgcg
    18181 attaacgatc ccaccgcaat cggtgccgat ctggcagcaa aacaggcaca acgtaacgag
    18241 ttctttattg tcggtgtgga cggaagcccg gacgccgaag aggcgctcaa gcgtgagaac
    18301 tcgttgtttg tcgctactcc ggcgcaagat ccccaggtga tggcagcgaa agcggtggaa
    18361 attggctacg acattttgca gggcaaacct gcgccgaaag agccggtgct gatcccggtg
    18421 acgatgatcg ataaaaacaa cgtcggcaat tacaaaggct ggacggtgaa ataagtccac
    18481 acgccgggcg gcgcagctgc ccggtcaccg ctaaggagaa cgctttcgat gaaacgctcc
    18541 gctataaacg acattctcgg ccatacacgg caattctttt cgcaacatga cgtacatctg
    18601 ccgccctttg ccagtttctc gcctgctcaa tggcagcagc tcgacaccgc cgcgtgggag
    18661 gaggtttttg atcttaaact cggctgggat gttaccgctt ttggtcgcaa caactttgca
    18721 gcacacgggc tgacgctgtt taccctacgt aacggctcag caaaaggaat gccgtatgtg
    18781 aaatgttacg ccgagaagat catgcatgtg cgcgatgcgc aggtcacgcc aatgcatttt
    18841 cactggcgta agcgtgagga cattatcaat cgcggcggcg gtaatttaat tgttgaactg
    18901 tggaatgccg atagtaacga gcaaacggct gatagtgaca ttacggtggt gatagacggc
    18961 tgccgccaga aacatactgc aggctctcag ttgcgcctgt ctcccggcga aagtatctgc
    19021 ctgccgcccg gcctgtatca cagcttttgg gcagaagccg gttttggcga tgtgctggtt
    19081 ggcgaagtct cttctgttaa tgacgacgac cacgataacc actttttaca gccactggac
    19141 cgctacaacc tgattgacga agacgaacct gcacagttgg tattgtgtaa cgaataccgc
    19201 cagttccgct gaataaggat aacgttatgc cattgatttc tctcgctgaa gggctggcac
    19261 atgcccgcga acatcactat tcgttaggtg cgtttaatgt gctggactcg cactttttac
    19321 gcgccttgtt tgctgctgcc agacaagaac gttcgccctt tattatcaac attgccgaag
    19381 tacattttaa atatatctcg ctggattccc tggtcgagac agtgaaattt gaagcggcgc
    19441 gtcatgacat tccggtggtg ttaaatctcg atcacggact gcattttgaa tcggtggtgc
    19501 aagcgttgcg gctgggattc agctcggtaa tgtttgatgg ctccacgctg gggtatgaag
    19561 aaaatgtccg ccagacgcgg gaagtggtga aaatgtgcca tgccgttggc gtatcggtag
    19621 aagctgaact gggcgcggtg ggtggcgacg aaggcggtgc actctatggt catgccgatg
    19681 aatccctgtt taccgatccg caactggcgc gtgattttgt cgaccgtacc ggcattgacg
    19741 cgctggccgt ggcgattggc aatgcacacg gcaaatatcg cggcgaaccg aaactcgatt
    19801 tcccgcgcct cgacgctatc cgccagcagg ctgctatccc gctggtatta cacggcggct
    19861 cagggattag cgatgccgat ttccgccgcg ccatcgaact gggtatccac aaaatcaatt
    19921 tctacactgg catgtcgcaa gcggcgctgg ctgccgttga ggcccgaatg gcgcaccgtc
    19981 agccgatgta tgacgagttt gccgagttac tgctggcagt ggaagccgcc atcagcgata
    20041 cggttgcaca acagatgcgt atttttggca gtgcggggca ggcatgatgg agcgtaaagg
    20101 gattatcgcc gcaggcaata tgttggttga ccacgtccat cagattatcc agtggccaga
    20161 gcgcggctgg ctggcggaga tcacccacag cgaacgctcc accggcggtg cgccgcttaa
    20221 cgtcctgctg acgctggcga aaatgcgcac cgggctgccg ttacaggcgg ttgggctaat
    20281 cggtcaggac agcgacggcg attacatcat ggcgatgctt gagcaatatc acgtcaatcg
    20341 tcagcatgtg cagcgcacta cctttgcccc gacgtcgatg tcgcaggtga tgacagaccc
    20401 aagcgggcag cggacgtttt tccattcgcc gggtgcaaac agacttttgg atcttccggc
    20461 gttcgatcag ctcgataaca ccatgaagat tttccatctc ggctatttac tgttgctcga
    20521 cagcctggat atgccggacg atgtttacgg cacccgtagc gcacgcctgc tggcgcaaat
    20581 gcgtgatact ggctttgaaa cctcactcga cctggtctcc cgtaagggcg atccgcgtta
    20641 tcagccgctg gtgctgcccg cgttgcgcca tctggattat ctggtaatta acgaactgga
    20701 ggccggggag tttagcgggc tggaaattcg gttacccaac ggcgaaccgc atatcgcgca
    20761 tattgctgcc gccgcgcgtg aactgttaga tgcgggcgtg cgccagcggg tggtgatcca
    20821 ctacccggaa ggggcgtggg gagagacccc agaacagggc ggcgtgtgga tcccttcgca
    20881 aaagttagaa caacgggaaa ttatcggcag cgtcggcgcg ggcgatgcat tttgtgccgg
    20941 atttttatat ggctgccatg aacgctggac gcttaccgag agcatcaaac tggcacacgc
    21001 ctgcgcgcgg gcaagcctgt tatgcgccaa tgccatcgac ggggcgaaaa cgctggaaga
    21061 gctgcaaaca gaaatgtcgg attaatgcac gtcggcagcg aagacatacc ccagaccgcg
    21121 cagagtttta atcaacatcg gctgatgcgg gttgagttcg attttgcgtc gtaagcgcat
    21181 gattaatacg tcgatggtgc gatcaaacac ctccacgctg tcgctgtggg tgaacgccag
    21241 caactgctcg cggctcaata cccgacgcgc gttttgcgcc agagccagta acaagccata
    21301 ttcgccctga gttaactcga catgttgctg aagcggattg tgcaactggc agcgcatggt
    21361 atcgagatac cagccattga atgacagccc ggcgcttttc tcgtcattaa ccgccagcgc
    21421 gccaacccgt cgcaacaccg ctttcagtcg tgcgaccacc acccgcgggg tgaacggttt
    21481 gcagatgtaa tcatccgcgc ccatttccag cccgaccacc acatcagact cactgcccat
    21541 gccggtcaac atcaccaccg gcaatgtcgg acgcagtttc tggatttgct gcaataccct
    21601 caggccgttg gtatcgggta gcatcatatc cagcaccacc agcgcaatgt caggatcgcc
    21661 ctcaatacgc aatatggcct cctgtccggt atggcagact gaaaccgtaa agacatgttc
    21721 acttaacacg tcctgcaaca gtgcgcagat agccgtgtca tcatccacca ccagcacgac
    21781 gggtttcatg gttacactcc gcatcattca tctgattaat cattgtgcgt acttcttgtc
    21841 gaagtgaaag ctgaaagtaa tataaataat aaaaata
//
LOCUS       DAZKFZ010000063        21032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000063 DAZKFZ010000000
VERSION     DAZKFZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..167
                     /locus_tag="ACHOQW_002191"
                     /pseudo
     CDS             1..167
                     /locus_tag="ACHOQW_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(192..506)
                     /gene="fliE"
                     /locus_tag="ACHOQW_002192"
     CDS             complement(192..506)
                     /gene="fliE"
                     /locus_tag="ACHOQW_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP9573270.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            721..2378
                     /gene="fliF"
                     /locus_tag="ACHOQW_002193"
                     /pseudo
     CDS             721..2378
                     /gene="fliF"
                     /locus_tag="ACHOQW_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            2371..3366
                     /gene="fliG"
                     /locus_tag="ACHOQW_002194"
     CDS             2371..3366
                     /gene="fliG"
                     /locus_tag="ACHOQW_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP9573271.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3359..4045
                     /gene="fliH"
                     /locus_tag="ACHOQW_002195"
     CDS             3359..4045
                     /gene="fliH"
                     /locus_tag="ACHOQW_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP9573272.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4107..5480
                     /gene="fliI"
                     /locus_tag="ACHOQW_002196"
     CDS             4107..5480
                     /gene="fliI"
                     /locus_tag="ACHOQW_002196"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP9573273.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5499..5942
                     /gene="fliJ"
                     /locus_tag="ACHOQW_002197"
     CDS             5499..5942
                     /gene="fliJ"
                     /locus_tag="ACHOQW_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP9573274.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5939..7066
                     /gene="fliK"
                     /locus_tag="ACHOQW_002198"
     CDS             5939..7066
                     /gene="fliK"
                     /locus_tag="ACHOQW_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP9573275.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7171..7635
                     /gene="fliL"
                     /locus_tag="ACHOQW_002199"
     CDS             7171..7635
                     /gene="fliL"
                     /locus_tag="ACHOQW_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP9573276.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7640..8644
                     /gene="fliM"
                     /locus_tag="ACHOQW_002200"
     CDS             7640..8644
                     /gene="fliM"
                     /locus_tag="ACHOQW_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP9573277.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8641..9054
                     /gene="fliN"
                     /locus_tag="ACHOQW_002201"
     CDS             8641..9054
                     /gene="fliN"
                     /locus_tag="ACHOQW_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP9573278.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9057..9422
                     /gene="fliO"
                     /locus_tag="ACHOQW_002202"
     CDS             9057..9422
                     /gene="fliO"
                     /locus_tag="ACHOQW_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP9573279.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9422..10159
                     /gene="fliP"
                     /locus_tag="ACHOQW_002203"
     CDS             9422..10159
                     /gene="fliP"
                     /locus_tag="ACHOQW_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP9573280.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10169..10438
                     /gene="fliQ"
                     /locus_tag="ACHOQW_002204"
     CDS             10169..10438
                     /gene="fliQ"
                     /locus_tag="ACHOQW_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP9573281.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10446..11231
                     /gene="fliR"
                     /locus_tag="ACHOQW_002205"
     CDS             10446..11231
                     /gene="fliR"
                     /locus_tag="ACHOQW_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9573282.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11521..12144
                     /gene="rcsA"
                     /locus_tag="ACHOQW_002206"
     CDS             11521..12144
                     /gene="rcsA"
                     /locus_tag="ACHOQW_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP9573283.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12188..12376)
                     /gene="dsrB"
                     /locus_tag="ACHOQW_002207"
     CDS             complement(12188..12376)
                     /gene="dsrB"
                     /locus_tag="ACHOQW_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP9573284.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12539..12766
                     /gene="yodD"
                     /locus_tag="ACHOQW_002208"
     CDS             12539..12766
                     /gene="yodD"
                     /locus_tag="ACHOQW_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP9573285.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13064..13879
                     /gene="yedP"
                     /locus_tag="ACHOQW_002209"
     CDS             13064..13879
                     /gene="yedP"
                     /locus_tag="ACHOQW_002209"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP9573286.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(13876..15570)
                     /gene="dgcQ"
                     /locus_tag="ACHOQW_002210"
     CDS             complement(13876..15570)
                     /gene="dgcQ"
                     /locus_tag="ACHOQW_002210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP9573287.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(15741..15923)
                     /gene="yodC"
                     /locus_tag="ACHOQW_002211"
     CDS             complement(15741..15923)
                     /gene="yodC"
                     /locus_tag="ACHOQW_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP9573288.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16002..16919)
                     /gene="yedI"
                     /locus_tag="ACHOQW_002212"
     CDS             complement(16002..16919)
                     /gene="yedI"
                     /locus_tag="ACHOQW_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP9573289.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            17092..18012
                     /gene="yedA"
                     /locus_tag="ACHOQW_002213"
     CDS             17092..18012
                     /gene="yedA"
                     /locus_tag="ACHOQW_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP9573290.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPANPVVAPVIQDASSE"
     gene            complement(18001..18471)
                     /gene="vsr"
                     /locus_tag="ACHOQW_002214"
     CDS             complement(18001..18471)
                     /gene="vsr"
                     /locus_tag="ACHOQW_002214"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP9573291.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18452..19870)
                     /gene="dcm"
                     /locus_tag="ACHOQW_002215"
     CDS             complement(18452..19870)
                     /gene="dcm"
                     /locus_tag="ACHOQW_002215"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP9573292.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(19937..20632)
                     /gene="yedJ"
                     /locus_tag="ACHOQW_002216"
     CDS             complement(19937..20632)
                     /gene="yedJ"
                     /locus_tag="ACHOQW_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP9573293.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(20672..20952)
                     /gene="drpB"
                     /locus_tag="ACHOQW_002217"
                     /pseudo
     CDS             complement(20672..20952)
                     /gene="drpB"
                     /locus_tag="ACHOQW_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         5051 a         5599 c         5440 g         4942 t
ORIGIN      
        1 taacaacatc cgacgtggtg atgtgtcaaa tgagacactc tgatgttcag ggggtttatc
       61 gtgtatacgg atcgtggatg gcagaaaact ttcaggatca ggtatccatt tcaaaccaga
      121 taatgagtaa gtttgcccca tccatgcccc acgcggtgag atcggatgtc attaacaaca
      181 ggttacacaa cctatacctg catgctcatt acttcctgat atgccgccac cagcttatta
      241 cgcacctgaa tccccatttg catagaaact gaggcttttt gcatatcggt catcacatcg
      301 tttaacgcca cgccgggttc accgagagtg aatttttctg cctgcgtgcg ggcggctgtt
      361 tgtgtatcgc ttatgcgatc gagcgcggcg tgcagctgcc cggcaaaact aatggtcggt
      421 tgcggcagtg attcctgcgc acgcgcactc atcgccgtag cctgtaactg gctgataacc
      481 ccttcaatcc cctgtatcgc tgacattttc atctcctgga taatttctgg tagcaaagcc
      541 taccagtaag tcaataagac aaaggcgcta aatagcaaca aaaaaacggg tttattggcg
      601 gatagaaaaa aacgaaagca caaataatgg gagcgtcaat ttttcgagtt tgctgacccg
      661 ggagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac gaggtgcgcg
      721 atgaatgcga ctgcagccca gacaaaatct cttggtggct taatcgcctg cgtgcgaatc
      781 cgaaaattcc attgattgtt gccggttccg cggcagtggc ggtcatggtc gcactgatcc
      841 tgtgggcgaa agcccccgac taccgcacat tattcagcaa tctttccgat caggatggtg
      901 gcgcaattgt cagccaactg acgcaaatga atattcctta ccgcttcagc gaagccagcg
      961 gcgctattga agttccggca gataaagttc acgagctgcg cctgcgcctg gcgcaacaag
     1021 gtttgcccaa aggcggcgcg gtcggtttcg aactactcga tcaggaaaag tttggtatca
     1081 gccagttcag cgaacaggtg aattatcagc gggcgctgga aggcgagctt tctcgtacca
     1141 tcgaaactat cggcccggta aaaggggcgc gcgtacatct ggcaatgccg aaaccgtctt
     1201 tattcgtccg tgaacaaaaa tccccttctg catcggtgac ggtaaatctg ttacccggcc
     1261 gcgcactcga tgaagggcaa attagcgcca ttgtgcatct ggtttccagc gccgttgctg
     1321 gtctgccgcc gggaaacgtc acgctggtgg atcagggcgg acatctgtta acccagtcca
     1381 ataccagctg gcgcgatctt aatgacgctc aattgaaata tgccagcgat gtcgaaggcc
     1441 gtattcagcg gcgtattgaa gcaatcctgt cgcctattgt tggtaacggt aatattcacg
     1501 cccaggtcac ggcgcaactg gacttcgcca gtaaagaaca aacggaagaa cagtatcgcc
     1561 ctaatggtga tgaatctcag gcggcgcttc gttcacgcca gcttaatgag agcgagcaaa
     1621 gcggttccgg ttatccgggc ggcgtaccgg gggcgttgtc gaatcaaccg gctcctgcga
     1681 ataacgcgcc aatcagcacg cctccggcaa atcaaaataa ccgccagcag caggcgagca
     1741 ccaccagcaa tagtgggccg cgtagcacac agcggaatga aaccagtaac tacgaagtcg
     1801 atcgcactat tcgtcatacc aaaatgaacg tgggcgatgt gcaacgtctg tcagtcgcgg
     1861 tagtggtgaa ttacaaaacc ttgccagacg gtaaaccatt gcctctcagc aacgaacaga
     1921 tgaaacaaat tgaagatctg acccgcgagg cgatgggctt ttctgaaaaa cgcggtgact
     1981 cgctcaatgt cgttaactcg ccgttcaata gtagtgacga aagcggcgga gaactgccgt
     2041 tctggcaaca gcaagcgttt atcgatcagt tactggctgc cggtcgctgg ttgctggtac
     2101 tgctggtggc gtggctgctg tggcgtaaag cggtacgccc gcagctaaca cgtcgcgctg
     2161 aggcgatgaa agctgtacag caacaggcgc aggcccgcga ggaagtggaa gatgcggtgg
     2221 aagtccgcct gagcaaagac gaacaactcc aacaacggcg cgctaaccaa cgtctggggg
     2281 cagaagtcat gagccagcgt atccgtgaaa tgtctgataa cgatccgcgc gtggtagcgc
     2341 tggtcattcg ccagtggata aataacgatc atgagtaacc tgacaggcac cgataaaagc
     2401 gtcatcctgc tgatgaccat tggcgaagac cgggcggcag aagtgttcaa gcacctctcc
     2461 cagcgcgagg tgcaaaccct gagcgctgca atggcgaacg tcacgcagat ctccaacaag
     2521 cagctaaccg atgtgctggc ggagtttgag caagaagctg aacagtttgc cgcactgaat
     2581 atcaacgcca acgattatct gcgttcggta ttggtcaaag ctctgggtga ggaacgtgcc
     2641 gccagcctgc tggaagatat tctcgaaact cgcgataccg ccagcggtat tgaaacgctc
     2701 aactttatgg agccgcagag cgccgccgat ctgattcgtg atgagcatcc gcaaattatc
     2761 gccaccattc tggtgcatct gaagcgcgcc caggccgccg atattctggc gttgttcgat
     2821 gaacgtctgc gccacgacgt gatgttgcgt atcgccacct ttggcggcgt gcagccagcc
     2881 gcgctggcgg aactgaccga agtactgaat ggcttgctcg acggtcagaa tctcaagcgc
     2941 agcaaaatgg gcggcgtgag aacggcagcc gaaattatca acctgatgaa aactcagcag
     3001 gaagaagccg ttattaccgc cgtgcgtgaa ttcgacggcg agctggcgca gaaaatcatc
     3061 gacgagatgt tcctgttcga aaatctggtg gatgtcgacg atcgcagcat tcagcgtctg
     3121 ttgcaggaag tggattccga atcgctgttg atcgcgctga aaggggccga gcagccactg
     3181 cgcgagaaat tcctgcgcaa tatgtcgcag cgtgccgccg atattctgcg cgacgatctc
     3241 gccaaccgtg gtccggtgcg tctgtcgcag gtggaaaacg aacagaaagc gattctgctg
     3301 attgtgcgcc gccttgccga aactggcgag atggtaattg gcagcggcga ggatacctat
     3361 gtctgataat ctgccctgga aaacctggac gccggacgat ctcgcgccac cacaggcaga
     3421 gtttgtgccc atggtcgagt cggaagaaac catcattgaa gaggctgaac ccagccttga
     3481 gcagcaactg gcgcaactgc aaatgcaggc ccatgagcaa ggttatcagg cgggtattgc
     3541 cgaaggtcgc cagcaaggtc ataagcaggg ctatcaggaa ggactggccc aggggctgga
     3601 gcaaggtctg gcagaggcga agtctcaaca agcgccaatt catgcccgga tgcagcaact
     3661 ggtcagcgaa tttcaaacta cccttgatgc acttgatagt gtgatagcgt cgcgcctgat
     3721 gcagatggcg ctggaggcgg cacgtcaggt catcggtcag acgccaacgg tggataactc
     3781 ggcactgatc aaacagatcc aacagttgtt gcagcaagaa ccgttattca gcggtaaacc
     3841 acagctgcgc gtgcacccgg atgatctgca acgtgtggat gatatgctcg gcgctacctt
     3901 aagtttgcat ggctggcgct tgcggggcga tcccaccctc catcctggcg gctgtaaagt
     3961 ctccgccgat gaaggcgatc tcgacgccag tgtcgccact cgctggcaag aactctgccg
     4021 tctggcagca ccaggagtgg tgtaatgacc acgcgcctga ctcgctggca agaactctgc
     4081 cgtctggcag caccaggagt ggtgtaatga ccacgcgcct gactcgctgg ctaaccacgc
     4141 tggataactt tgaagccaaa atggcgcagt tgcctgcggt acgtcgctac gggcgattaa
     4201 cccgcgctac cgggctggtg ctggaagcca ccggattaca attgccgctc ggcgcaacct
     4261 gtgtcattga acgccagaac ggcagcgaaa cgcacgaagt agaaagcgaa gtcgttggct
     4321 ttaacggtca acggctgttt ttaatgccgc tggaggaagt ggaaggtgtc ctgcccggcg
     4381 cgcgggttta tgccaaaaac atttcggctg aaggactgca aagcggcaag cagttgccgc
     4441 tcggtccggc gttattaggt cgcgttctgg acggcagcgg taaaccgctc gatggcctgc
     4501 cctcccccga tacgacggaa accggtgcgc tgattacccc gccatttaac ccgttgcaac
     4561 gtacaccgat tgaacatgtg ctggacaccg gcgtgcgccc aatcaatgcc ctgcttaccg
     4621 ttgggcgtgg gcagcgtatg gggctgtttg ccgggtccgg cgttggtaaa agtgtgctgc
     4681 tggggatgat ggcacgttac acccgcgccg atgtcattgt cgtgggtttg attggtgaac
     4741 gtgggcgcga agtaaaagat tttattgaga atatcctcgg tgccgaaggg cgtgcacgat
     4801 cagtggtgat tgccgctccg gcggatgtgt ctccgctcct gcgaatgcaa ggtgccgcct
     4861 atgccacgcg cattgccgaa gattttcgcg atcgtggtca gcatgtgttg ctgattatgg
     4921 actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt ggcgaaccac
     4981 ccgccaccaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg
     5041 ccggaaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg cttactgaag
     5101 gcgatgacca gcaggacccc attgcagact ccgcgcgggc gatcctcgac ggtcacattg
     5161 tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt gaagcgtcga
     5221 tcagccgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca
     5281 aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca
     5341 aaggcagcga tccgatgctc gacaaagcca tcgccctgtg gccgcagctg gagggctatt
     5401 tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg ctggagcgta
     5461 ttttcccgac agtgtcataa cccaggagat aacggcagat ggcagaacat ggcgcgctgg
     5521 cgaccctgaa agatctggca gaaaaagagg tagaggatgc cgcgcgcctg ctgggtgaaa
     5581 tgcgtcgcgg gtgtcagcag gcggaagaac agctcaaaat gctgatcgat tatcagaatg
     5641 aatatcgcaa taacctcaac agcgatatga gtgccgggat gaccagcaac cgctggatca
     5701 actatcagca gtttatccag acgctggaaa aagccattac tcagcatcgc cagcaactta
     5761 atcagtggac gcagaaagtt gacattgccc tgaacagctg gcgagaaaag aaacaacgtt
     5821 tgcaggcctg gcagacactg caggaacggc aatccacggc ggcactgctt gcagaaaacc
     5881 gcctcgatca gaaaaagatg gatgagttcg cccagcgcgc cgccatgagg aaacctgaat
     5941 gattcgctta gcgcccttga ttaccgccga cgttgacacc accacattgc ctggcggcaa
     6001 agccagcgat gctgcacaag attttctcgc gttgttgagc gaagcattag caggcgagac
     6061 aactaccgac aaagcggccc cccagttgct ggtggcaaca gataagccca cgacaaaagg
     6121 cgagccgctg atcagcgata ttgtttccga cgcgcaacaa gctaatttac tgatccctgt
     6181 ggatgaaaca ccgcctgtca tcaacgacga acaatccaca tcaacaccgt taaccaccgc
     6241 tcagacgatg gcgttggctg cggtggctga caaaaatacg acaaaagacg aaaaagcgga
     6301 tgatctgaat gaagacgtca ccgcaagcct gagcgccctt tttgcgatgt tgccgggttt
     6361 tgacaatacg cccaaagtga ctgatgtgcc gtcaaccgtg ttaccggcag agaaaccaac
     6421 gctcttcaca aaactgactt ctgcgcaact cacaacagca cagcctgatg atgcccccgg
     6481 cacaccagct cagccattaa caccgcaggt agcagaagcc cagagtaaag cggaagtcat
     6541 cagcacgcct tcgccggtga ctgctgccgc cagcccgcta atcactccac accagacaca
     6601 gccactgccc accgtcgccg cgcctgtttt gagtgcaccg ctgggttctc acgaatggca
     6661 acaatcatta agccagcata tttcgctgtt cacccgccag gggcaacaaa gtgcagagtt
     6721 gcgtctgcac ccgcaggatt taggtgaagt gcaaatctcc ctcaaagtgg atgataacca
     6781 ggcgcaaatc cagatggttt catcgcatca gcatgtacgc gccgccctgg aagcagcgct
     6841 gccggtactg cgtacgcagc tggccgaaag tggcattcag ttagggcaaa gcaacatcag
     6901 tggcgaaagc tttagtggtc agcagcaggc cgcttcccaa caacagcaaa gccaacgcac
     6961 agtaaaccat gaacctctgg cgggggaaga cgacgatacg cttccggttc ccgtctcttt
     7021 acaagggcgc gtaacaggca acagcggcgt tgatattttc gcctaacgtc agaggtagca
     7081 ccgtaatccg cgtcttttcc ccgctttgtt gcgctcaaga cgcaggataa ttagccgata
     7141 agcagtagcg acacaggaag accgcaacac atgactgatt acgcgataag caagaaaagc
     7201 aagcgatcgc tttggatccc gattctggta ttcattaccc tcgcggcctg tgccagcgca
     7261 ggttacagct actggcattc gcatcaggtt gctgctgacg acaaagcgca gcaacgcgtc
     7321 gtgccctccc cggtcttcta tgcgctggat acctttacgg tcaatctggg cgatgcggat
     7381 cgcgtgcttt atatcggcat aaccctgcgc ctgaaagatg aagctacccg ctcgcggctg
     7441 agtgagtatt tgccggaagt ccgtagccgt ttgctgttac tgttttcgcg tcaggatgct
     7501 gccgtactgg cgacagaaga aggcaagaaa aacctgattg ccgagattaa aaccacactt
     7561 tccaccccgc ttgttgccgg gcaaccgaaa caggatgtca ccgacgtgct gtataccgct
     7621 tttattctgc gataacgaca tgggcgatag tattctttct caagctgaaa ttgatgcgct
     7681 gttgaatggt gacagcgaag tcaaagacga accgacagcc agtgttagcg gcgaaagtga
     7741 cattcgtccg tacgatccga atacccaacg acgggttgtg cgcgaacgtt tgcaggcgct
     7801 ggaaatcatt aatgagcgct ttgcccgcca ttttcgtatg gggctgttca acctgctgcg
     7861 tcgtagcccg gatataaccg tcggggccat ccgcattcag ccgtaccatg aatttgcccg
     7921 caacctgccg gtgccgacca acctgaacct tatccatcta aaaccgctgc gcggcactgg
     7981 gctggtggtg ttctcaccga gtctggtgtt tatcgccgtg gataacctgt ttggcggcga
     8041 tggacgcttc ccgaccaaag tggaaggtcg cgagtttacc cataccgaac agcgcgtcat
     8101 caaccgcatg ttgaaactgg cgcttgaagg ctatagcgac gcatggaagg cgattaatcc
     8161 gctggaagtt gagtacgtgc gttcggaaat gcaggtgaaa tttaccaata tcaccacctc
     8221 gccgaacgac attgtggtta acacgccgtt ccatgtggag attggcaacc tgaccggcga
     8281 atttaatatc tgcctgccat tcagcatgat cgagccgcta cgggaattgt tggttaaccc
     8341 gccgctggaa aactcgcgta atgaagatca gaactggcgc gataacctgg tgcgccaggt
     8401 gcagcattca cagctggagc tggtcgccaa ctttgccgat atctcgctac gcctgtcgca
     8461 gattttaaaa ctgaaacccg gtgacgtcct gccgatagaa aaacccgacc gcatcatcgc
     8521 ccatgttgac ggcgtcccgg tgctgaccag tcagtacggc actctcaacg gtcagtatgc
     8581 gttacggata gaacatttga ttaacccgat tttaaattct ctgaacgagg aacagcccaa
     8641 atgagtgaca tgaataatcc ggccgatgac aacaacggcg caatggacga tctgtgggct
     8701 gaagcgttga gcgaacaaaa atcaaccagc agcaaaagcg ctgccgacgc ggtgttccag
     8761 caatttggcg gtggtgatgt cagcggaacg ttgcaggata tcgacctgat tatggatatt
     8821 ccggtcaagc tgaccgtcga gctgggccgc acacgaatga ccatcaaaga gctgttgcgt
     8881 ctgacgcaag ggtccgtcgt ggcgctggac ggtctggcgg gcgaaccact ggatattctg
     8941 atcaacggtt atttaatcgc ccagggcgaa gtggtggtcg ttgccgataa atatggcgtg
     9001 cggatcaccg atatcattac tccgtctgag cgaatgcgcc gcctgagccg ttagtgatga
     9061 ataaccacgc tactgtgcaa tcttccgcgc cggtttctgc tgcgccactg ctgcaggtga
     9121 gcggcgcact catcgccatt attgccctga tcctcgctgc tgcctggctg gtaaaacggt
     9181 tgggatttgc cccgaaacgc actggcgtta acggtctgaa aattagcgcc agtgcttcgc
     9241 tgggcgcgcg tgaacgggtt gtggtggtcg atgtggaaga tgcacggctg gtgctcggcg
     9301 ttaccgcagg gcaaatcaat ctgctgcata aacttccccc ttcagcacca acagaagaga
     9361 taccgcagac cgattttcag tcggtcatga aaaatttgct taagcgtagc gggagatcct
     9421 gatgcgtcgt ttattgtctg tcgcacctgt ccttctctgg ctgattacgc ccctcgcctt
     9481 cgcgcaactg ccgggtatca ccagccagcc gctgcctggc ggtggacaaa gctggtcgct
     9541 cccggtgcag acgctggtgt tcatcacctc gttgacgttt attccggcaa ttttactgat
     9601 gatgaccagt ttcacccgca tcatcattgt ttttggttta ttgcgtaacg cgctgggaac
     9661 accctccgcg ccacctaacc aggtattgct ggggctggca ctgtttttga ccttttttat
     9721 tatgtcaccg gtgatcgaca aaatttatgt cgatgcgtac cagccattca gcgaagagaa
     9781 aatatcaatg caggaggcgc tggaaaaagg ggcgcagccg ctgcgtgagt ttatgctgcg
     9841 tcagacccgt gaggcagatt tagggttgtt tgccagactg gcgaataccg gcccgttgca
     9901 gggacctgaa gccgtgccga tgcgcatttt gctcccggcc tacgtgacca gcgagttgaa
     9961 aaccgcattt cagataggct tcacgatttt catccctttt ttgattatcg acctggtgat
    10021 agccagcgtg ttgatggcat tggggatgat gatggttccc cccgccacca ttgctctgcc
    10081 ctttaaactg atgctgtttg tactggtgga tggctggcaa ttgctggtcg gttcgctggc
    10141 gcagagcttt tacagctaga gaggcaaaat gacacctgaa tcggtcatga tgatggggac
    10201 tgaagcgatg aaagtcgcgc tggcactggc tgccccgcta ttgttggtag cgttggtcac
    10261 gggccttatc atcagtattt tgcaggccgc cacgcagatt aacgaaatga cgctgtcgtt
    10321 tattccgaaa atcatcgccg tatttatcgc cattattatt gccggaccgt ggatgctcaa
    10381 tctgttgctg gattacgtcc gcaccttgtt cactaacctg ccgtatatca tcgggtagcc
    10441 gtactatgtt gcaggtgaca agcgaacaat ggctatcctg gttaagcctg tacttctggc
    10501 cgttactgcg cgtgctggcg ctgatctcca ccgcgccgat tctgagcgaa cgcagcgtac
    10561 cgaaacgggt aaaactgggt ctggcaatga tgatcacgtt cgccattgcc ccatcattac
    10621 ctgccaacga tgttcctgtt ttttcgttct ttgctctgtg gctggccgtg cagcagatcc
    10681 tgatcggcat tgcgcttggt tttaccatgc aatttgcctt tgccgctgtg cgaaccgctg
    10741 gcgaaattat cggtctgcaa atggggctgt catttgcgac gtttgtcgat ccggccagcc
    10801 atcttaatat gcccgtttta gcgcgtatca tggatatgct ggcgttactg ctgttcctga
    10861 catttaacgg tcatttatgg ttgatttcac tgctggtcga tacctttcac accctgccga
    10921 ttggtggcga accgttgaac agcaatgcgt ttctggcact caccaaagca gggagtttga
    10981 ttttccttaa cgggctgatg ctggcgttac cgctcattac tctgctgctg acactgaatc
    11041 tggcattagg tttacttaat cgtatggccc cgcaattatc catttttgtt attggatttc
    11101 cattaactct gactgtcggc atctctttaa tggcggcatt aatgccgtta attgcgcctt
    11161 tttgcgaaca tttattcagt gaaattttta atttgctggc tgatattatt agtgaattgc
    11221 cattaatata attccgtaac gtttatcatg ttatcctaag gattatccga aaaataatac
    11281 ctacgaacat cttccaggat actcctgcag cgaaatattt gttttaagct cactcacata
    11341 tcgcaacatt tactttactt taagacaatt ccaggcaaat tatacaacac tttacgggat
    11401 agtaagtccg cctgaaaaat cgcgagagtg gcgcattagg tgacccatgt tgttccgttt
    11461 agtcatgatg aaatattcag gtaaggggaa ttatcgttac gcattgagtg agggtatgcc
    11521 atgtcaacga ttattatgga tttatgtagt tacacccgac taggtttaac cgggtatctg
    11581 ttgagtagag gggttaaaaa aagagaaatc aacgacattg aaaccgttga tgaccttgcc
    11641 atagcttgtg attcacagcg cccttcagtg gtgtttatta atgaggactg tttcatccac
    11701 gatgcttcta acagtcagcg tatcaagctc atcattaatc aacatcccaa tacgttattt
    11761 atcgttttta tggcaattgc caatgttcat tttgatgaat atctattggt cagaaaaaat
    11821 ttattgatca gttctaaatc gattaaaccg gaatctctcg acgatatcct tggcgatatt
    11881 ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc cgacgttatc attgagccga
    11941 accgaatcga gtatgttgcg aatgtggatg gcaggtcagg gaaccattca aatctccgac
    12001 caaatgaata tcaaagccaa gaccgtttca tcgcataaag gtaatattaa acgtaagatc
    12061 aaaacgcata ataaacaggt tatctaccat gtcgtccgac tgacggataa tgtgactaat
    12121 ggtatttttg tcaacatgcg ctaacacatt ctgactggtg gtttcccacc agtcaggctg
    12181 aattagatta ctctgctttc tccacaaaga taccgtcctg atgccctgct tcattaaaga
    12241 accagatgcc gagcgggtag tcttccagcg aaaccaggta cattgtgcct tcactaaact
    12301 cctcaactgc cagtaccacg ccaggacgac gcggaccgcc atccgttttg actgttaccc
    12361 gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc cagtgtagaa caatttcgtt
    12421 tttctggcag cgccaggcgc gcgcgagtgc tgattttctc gacggtctat acttaagaga
    12481 tgccaggcgg acttaacgac tggcggcaac aacagagtaa cggttgcgag gaaagatgat
    12541 gaaaaccgca aaagagtaca gcgataccgc aaaacgtgag gtcagcgtcg atgtcgatgc
    12601 cctgctggcg gcgatcaatg aaattagcga aagcgaagtt catcgcagcc agaacgattc
    12661 tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg cgtgaattgg cggatgcctt
    12721 cgaactggat attcatgact tcagcgtctc tgaagtgaat cgttgaatgc acaataaaaa
    12781 aatcccgacc ctgagggggt cgggatgaaa cttgcttaag caagaagcac ttaaaaaatt
    12841 cgttacacca ggaaatctga tgtgttcatc accttatccg caattttttt cgctgacaag
    12901 aaaatattcg ctatatgaat gattaacgct acttatgaat agaaatgtga cgcacgtcac
    12961 cccttggtgg ctctggcgtc agcgggatcg ctcccgaaaa aatcatcttt gataatttgt
    13021 gctgttttga gaatcatcct ggtaagggga gtactgcgcg actatgtttt caattcaaca
    13081 accactactg gtttttagcg atcttgatgg caccctgctg gacagtcata gttatgactg
    13141 gcaaccggca gccccctggc tcagccgttt acgcgaagca aatgttcccg tcattctctg
    13201 tagcagtaaa acatcagcgg aaatgctgta cttgcaaaaa atgttggggc tacaaggttt
    13261 accgctgatt gcagagaatg gcgcagtgat ccagcttgct gaacaatggc aggagataga
    13321 cggttttcca cgcatcatct caggtattag ccatggcgaa atcagccagg ttttaaatac
    13381 gctacgcgag aaagaacatt ttaaattcac gacttttgat gatgtcgacg atgcaaccat
    13441 cgccgaatgg acgggattaa gccgtagcca ggcggcgctg acgcagcttc atgaggcgtc
    13501 ggtaacgcta atctggcgcg acagtgacga gcgtatggca caatttaccg ctcgtctgaa
    13561 cgaactgggc ttacagttta tgcaaggtgc gcgcttctgg cacgtcctgg atgcctctgc
    13621 cggaaaagat caggctgcca actggattat cgcgacctat caacaattgt caggcaaacg
    13681 cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg cccttactgg aggtaatgga
    13741 ttacgcggtg attgtgaaag ggctaaaccg tgaaggggtg catctgcatg atgaggatcc
    13801 ggcccgcgtc tggcgaacgc agcgtgaagg accggaagga tggcgtgaag ggctggacca
    13861 ttttttctcc gcccgttaag cgttatcgct cgcgaatacc cgattgcgcc cagcctgttt
    13921 agcgagataa agccgacggt cggccagtga ctggagttgt tcaaaatcat aatcaccggt
    13981 ttcctcgctg ctacttaccc ccaacgaggc actgatgcgt atcgtcgtac tcttagcaat
    14041 caacatctct ttttcattta acttcaggcg aatacgttct gcgacttccg cagcctccgt
    14101 cagactcgcg cctggcagaa tcacacaaaa ctcctcacca ccgacccgcc cggcaacgtc
    14161 ctgcgcacgc aaggaactgc taattaatcc ggcagcatga gaaagaacac ggtcgcccgc
    14221 ctgatgacca aagcggtcat taatcgcttt aaaatggtca agatcgacct gaatgacaga
    14281 aaaaggatgt tggtgcgtct gacacaattt cgcgagcgga cgggcttttt cgaacagtgc
    14341 gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc tgccactgca acgagctttg
    14401 cagaacatac atgttgctga ccatccggcg aatcacatac caggagatga gtaacatggt
    14461 ggtaaagagc gcccacagca gggttaatgc aatgctgata ctgccgaaat cgccgcgcac
    14521 gccttcgctt aacgtatgga cgcgcaccag cacaccgtcg aaatggtcca gacgttccca
    14581 gctaacatag cgactgtcca tacgaatgcc gccccgcgtg tcatgttcca ttgcctgcgc
    14641 cagcaaggcc agttcacgag gatcaaaaat attccctgtt ggatggtcag gattggaaga
    14701 ggtcaaaaat ctcagcttac tgtcatagag ctgatactca ccatcgaggt ttttatcgat
    14761 ggcgtttctt aaaaattgct gcatagtacg cacgggaata ctcatcccca gcacgccata
    14821 ccagtagtta ttactgtcta ccggaacact gacggtaacc tgcggttcag tattgctggc
    14881 gtgttccggt tgcgaagtga accagcgtac cgcgcggtga cgattttctc gttgtgaatg
    14941 gccgataaac cagggttggg tgacatagcc gtaataacgc gtcgggacat tgcgcgtaaa
    15001 caaggtcggc tgcgtcgaaa cgtaaaatcc ggcacgcgag acatacatcg cctgttctac
    15061 catcgacgag gagttgtgtg ccagtcgcag caagtaacca acttccagtg cagcggtaat
    15121 ttcattgtcg aggctttcat tttcgcgaga caggagattc ccctcgctga ctaatgcatc
    15181 cgatacgcca ttgactgaca gggtgcgtcg tcggttgagt tcgatttgcc aggcgtgctc
    15241 gtcgcgatgc tgttcgaact cggttacagc atcacgcaga gaggtgaaat ccagtggcgc
    15301 tacgagagct tcgcgcatgc cattacgcaa aaagatcagt ttgtcgacgt tatactgcaa
    15361 atgcttatcg agcgcgttgg ctacgttttc cagatgatta cgctggctgg agatataggc
    15421 atcttccagc accactactt cacgccaggt cagcaaggtg gaaaaaagca ataccacgat
    15481 aaagcagaga ttaacgatat gaccaggccc taggcggcgt gcgagttttt tcaaccagct
    15541 ctggttttcc atttttgtct cgtgctgcac cctgacgact ccccacctgc ttttttatga
    15601 ttctggcata acctggcgat agcgtaccgc aaaacagcga taattttata atggtggcat
    15661 gataagacgc gactatcatt cattgcaggc attggcacac caggccggat gtgatccggc
    15721 ctggtcttaa tgataccctt tcaaacttct tccgcagaac gactcccctc ccctggaaca
    15781 agctcggttt catgaaaagc ttcccgcttg accccgtaac cgtcatacca acgacactca
    15841 accataccgc tggagtatcc agtgacaatc atccgcgggc cgccctcttt aaccgtaact
    15901 tcctcactaa ccataaagct catactcgcc tccttttttc gagtgaaact tgttcacctt
    15961 agttgaagat ggcgaatttt gcataatagc ctgtgcgagt attaatgtac ctgaccgcgc
    16021 attttcgcta cggctttcac tcccagcacc acgatgccgc cgatgataaa tccaagaatc
    16081 agatttaaaa cagtcggtaa tatcatcgcc accactgcac tttgctgccc ggcgaaatgt
    16141 tcaatggcgt gatgcagcgg cgcaatacca tgtaccacaa tcccgccgcc gacgaggaac
    16201 atcgccagcg tgccgacaat cgataacgct ttcatcagcc agggcgcgat aatcaataat
    16261 cctttaccta atgcctgcat cagcgcgctg gatttttccg ccagccaata ccccaggtca
    16321 tcaatcttaa cgataacccc taccagaccg tagacgccca cggtcactac cagcgcgatg
    16381 cctgaaagca ccagcacctg attaagcaac ggcgcttcgg ccacaatccc cagcgtgatg
    16441 gcgacgattt ccgcagacaa tataaaatcg gtacgaatcg ccccttttat tttgtccttt
    16501 tcaaacttca gcggatcctg cgccgccagc ttctccagac gctgctggct ctgcgccgga
    16561 tcttctttat gtttacgcgc ctccagcata tgcagcactt tctctacccc ttcaaagcag
    16621 agaaacgcgc caccgatcat caacaatggc gtaatcgccc acgggataaa cgcactgatg
    16681 atcagcgcca gtggcaccag aatcacttta ttaatcagcg atcctttcgc cacgccccag
    16741 accacgggaa gttcccggtt ggcccgcacg cctgaaactt gttgcgcatt gagcgataag
    16801 tcatccccta atacaccggc ggttttcttc gccgccagtt tgcccatcac ggagatatcg
    16861 tccagcagtg tggcgatatc atcgagcaac gtcagtaaac tactcccggc caaaagcatt
    16921 ccctctcatt tttattgttg aagggtgtaa gtatgaagca aaaacggcag ctctgatact
    16981 tgcttcacag gtcaacattt ttttaacaat caagtgcaat tatatctctc gccagcagaa
    17041 tgattttcac gtttactatg aaccaccttt ttatttccac cgtgagggat tatgcgtttc
    17101 cgccagttgt taccgctttt tggcgcgctg tttgcgttgt atatcatttg gggctcaacc
    17161 tattttgtca ttcggattgg cgtggaaagc tggcctccgt taatgatggc gggtgttcga
    17221 ttcctggcag ccggtatttt attgctggca tttttactac tgcgcggaca caaactcccc
    17281 ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt tattgctggc tgtcggtaat
    17341 ggcatggtga cggttgccga acatcaaaat gttccttccg gcatcgccgc cgtagtggtt
    17401 gcaaccgtgc ccctctttac cctgtgcttc agccgcctgt ttggcattaa aacgcgcaaa
    17461 ctggaatggg tgggtattgc cattgggctt gccggaatca tcatgctcaa tagcggtgga
    17521 aatttaagcg gcaatccgtg gggcgcgatt ctgattttaa tcggctcgat tagctgggcg
    17581 tttggctcag tttatggctc gcgcattacc ttacctgtag ggatgatggc gggtgcgatt
    17641 gagatgctgg cggcaggcgt ggtgttaatg atcgcgtcga tgattgcggg tgaaaaactg
    17701 acggcgctcc cttccctttc aggcttcctt gcggtcggct atctggcgct gtttggttcg
    17761 attatcgcca tcaacgctta tatgtattta atccgtaatg tcagtccggc tctcgccacc
    17821 agctacgctt acgttaaccc ggtggtcgcg gtcttgctgg gtacgggact gggtggagaa
    17881 acactgtcga agattgaatg gctggcgctc ggcgtaattg tcttcgcggt ggtactggtc
    17941 acgttgggaa aatatctctt tccggcaaac cccgtagttg ctccagttat tcaggacgca
    18001 tcaagcgagt aaatgtatcc cctgcgtgtc gatctgcgcg ctggctcctt cgccgcagat
    18061 ccactcttcc agacgctcgg taagcgcttc atccttcagc ttctcgcgcc cacgtaacgc
    18121 gcactcccag acaatcaata cgcgccagcc gagttcctgc aagcgactga tatcgcggcg
    18181 atcgcgctca acatttttac ctatcttctc cagccagaac tcggttcgag tcgcaggcac
    18241 tttaaacaga tagcagtgat gatgatgcca gaagcagcca tgggtaaata tcacgcagcg
    18301 atattcatca acgacaaaat ccgggcgtcc gggcagactg gcgtcctgaa cgcgaaacgc
    18361 caggccctgc ccggttaaca gactggcgag gcgcttctca atcgccgtat cacgcgtggc
    18421 aatcgcgcgc atatttttgc tgcgagtggc cttatcgtga acgtcggcca tgttgtgcct
    18481 cttgctgacg caacgccacc gcctgtttga tttttggctc aagcagtttt gccaccgcgg
    18541 caaagaccgg cacgaccacc gagttaccga actggcgata ggcctgagtg tccgaaaccg
    18601 gaatgcggaa tttcgcttct cccggcgctt caaaacccat taagcgcgcg cattcccgag
    18661 gcgttaaccg acgtggacga tgttgctgat tcagcggatc gtcaaagtct ttctcacccg
    18721 tggccatatc ccagccgcga tcgattaaaa tttccgcgcc atctttgtaa taacgcgcag
    18781 acagcgtacg ggtgacgctt tgcggattgt tcggataaac cattccataa ccgaagccgt
    18841 ttccgcgcgc ctgatgtttt ttcgcatatc gatagaggta cttccacagc accggcgtca
    18901 ggatatattt cgcctcgacc atcgggtcca acagctgcgc cagcgtcact cgctgtgcag
    18961 ggaaacattc gctgatatca cgcagagtaa aatcggcttt cagattaaga tcgcgacgaa
    19021 aacccaccag cacgatgcgt tcacggtgct gcggcagaaa atgtttgcca tcgatgattt
    19081 tcggatcgtc cggcccgtta tcttctgcat cagccacgtc atagcccagt tcgtccagcg
    19141 tctgcatgat gatgcggaac gttttaccct ggtcgtggct tttcaggttt ttgacgtttt
    19201 cgagcacaaa catcgccgga cgacgcgcgt cgataatgcg caccacatca aagaacagcg
    19261 tgccctgagt atcgcaggca aaaccgtgcg cccgcccgag cgagttcttt ttcgatacgc
    19321 cagccagcga aaatggctga caagggaaac cggccagtaa aacatcgtgt tcaggaatgt
    19381 gttgacgaat atgttccgcc gcagcctcat cactcacgcc ttctttatgg ctgagggtga
    19441 tgtcgcggat atcttcatta aaatgatgcg tcgccgggtc gcaataatga ttggctttat
    19501 aagtgcgtac cgcatgtttg ttccattcgc tggtaaacac gcactgtccg ccaatcgatt
    19561 caaaaccgcg acggatgccg ccaattccgg caaatagatc gataaagcga aacgcataat
    19621 gcggatgatg tgccggtggt ttgggtaaca acgtttgcag atgggcgaac tctttctcac
    19681 ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt taaaattgcc gcgctccagt
    19741 gattctcacc cacaccatta agctgagcca ccagcgtttt aacatcataa atttgcagca
    19801 gtttctccag cattgcctgt gcggcattcc cggtgctgta tgaatcggtt actgatatat
    19861 tttcctgcat agatttaacc ggccatctga gatggtcgac aggttaacat aattacagcc
    19921 atttagggcc acgcgctcag cccgcgggtg aaaacgcatc tatcaccttg tgatcgacac
    19981 cttcattctc ccccgccagt tcggcactga gcttcgccat aaactcgact aaaaaatgcg
    20041 cgttgtgctg cgccagctgc ttgcctctta cggtttgcat gatttgcggt agcttcaaca
    20101 acttagtctg gaaatgatcc agcgcgtagc gtttatcatc aagcgggcga tgctgtgcga
    20161 gcggatcttc ggcatcaaac agcgccacgc ccaatgcccc tgaaacggca aacacgcgcg
    20221 ccaggccaat cgcccccagg gcttccagcc gatccgcatc ctggacgatt ttagcttccg
    20281 ttgttaaggg ggcgatttgc gcgctgaaac tgtgagcggc aatggcatga caaacggcct
    20341 cgattttctc cgccggaaat tgcacgaact cttcacggag caggcgacgc gtctcttctg
    20401 ccgccaggat tgaagaactc tgccgttgcg gatgattttt cgccaggctc acaatatcgt
    20461 ggaaataaca ggcggttaaa atcaccagca tatcgacgtc atcatctgcc gccagcttct
    20521 gtgccgtagc ccagacgcgg cgaaaatgac acacgtcatg ggccgcgtcc tggtgctgat
    20581 gatggttttt caaccagttt tcgaactgtg cctgccagtg ttgtaagtcc atacgcgctc
    20641 cgttgccaaa gagtcgccac cttagcagtt tttattcata gcgtctgcta cgtgcgggac
    20701 gaggtcgtgt gtaccaggca atgctgccta acaatgcccc gaccagagcc ataaataaaa
    20761 atccgaccag tgccctaacc actttcccgc ggtagagcct aaatcgcttt caaatcccga
    20821 cacgggggca tcggggatcc ggctcatcac ccagatgtaa cgcgccattg cccagacgaa
    20881 atagccacac catgtataaa aagcccacaa agccagtttg ccgccagggc tgcgagagag
    20941 tctctcttcc atctttgtcg aaccgtattc cattttgaga taacccggaa aattcagact
    21001 tagtactatt acgtgataaa agtcacattt tt
//
LOCUS       DAZKFZ010000064        20143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000064 DAZKFZ010000000
VERSION     DAZKFZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHOQW_002218"
     CDS             complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHOQW_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9573294.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHOQW_002219"
     CDS             complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHOQW_002219"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP9573295.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHOQW_002220"
     CDS             complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHOQW_002220"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP9573296.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4659..5396
                     /gene="bamD"
                     /locus_tag="ACHOQW_002221"
     CDS             4659..5396
                     /gene="bamD"
                     /locus_tag="ACHOQW_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP9573297.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5667..6008
                     /gene="raiA"
                     /locus_tag="ACHOQW_002222"
     CDS             5667..6008
                     /gene="raiA"
                     /locus_tag="ACHOQW_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9573298.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6111..6236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6112..6159
                     /gene="pheL"
                     /locus_tag="ACHOQW_002223"
     CDS             6112..6159
                     /gene="pheL"
                     /locus_tag="ACHOQW_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP9573299.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6258..7418
                     /gene="pheA"
                     /locus_tag="ACHOQW_002224"
     CDS             6258..7418
                     /gene="pheA"
                     /locus_tag="ACHOQW_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP9573300.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHOQW_002225"
     CDS             complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHOQW_002225"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP9573301.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHOQW_002226"
     CDS             complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHOQW_002226"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP9573302.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9873..10238
                     /gene="yfiL"
                     /locus_tag="ACHOQW_002227"
     CDS             9873..10238
                     /gene="yfiL"
                     /locus_tag="ACHOQW_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP9573303.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10388..10906
                     /gene="yfiR"
                     /locus_tag="ACHOQW_002228"
     CDS             10388..10906
                     /gene="yfiR"
                     /locus_tag="ACHOQW_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP9573304.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10896..12122
                     /gene="dgcN"
                     /locus_tag="ACHOQW_002229"
     CDS             10896..12122
                     /gene="dgcN"
                     /locus_tag="ACHOQW_002229"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP9573305.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12138..12620
                     /gene="yfiB"
                     /locus_tag="ACHOQW_002230"
     CDS             12138..12620
                     /gene="yfiB"
                     /locus_tag="ACHOQW_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9573306.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12697..13044)
                     /gene="rplS"
                     /locus_tag="ACHOQW_002231"
     CDS             complement(12697..13044)
                     /gene="rplS"
                     /locus_tag="ACHOQW_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9573307.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13086..13853)
                     /gene="trmD"
                     /locus_tag="ACHOQW_002232"
     CDS             complement(13086..13853)
                     /gene="trmD"
                     /locus_tag="ACHOQW_002232"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9573308.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13884..14432)
                     /gene="rimM"
                     /locus_tag="ACHOQW_002233"
     CDS             complement(13884..14432)
                     /gene="rimM"
                     /locus_tag="ACHOQW_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9573309.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14451..14699)
                     /gene="rpsP"
                     /locus_tag="ACHOQW_002234"
     CDS             complement(14451..14699)
                     /gene="rpsP"
                     /locus_tag="ACHOQW_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9573310.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14836..16197)
                     /gene="ffh"
                     /locus_tag="ACHOQW_002235"
     CDS             complement(14836..16197)
                     /gene="ffh"
                     /locus_tag="ACHOQW_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9573311.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16364..17155
                     /gene="ypjD"
                     /locus_tag="ACHOQW_002236"
     CDS             16364..17155
                     /gene="ypjD"
                     /locus_tag="ACHOQW_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP9573312.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17177..18463
                     /gene="yfjD"
                     /locus_tag="ACHOQW_002237"
     CDS             17177..18463
                     /gene="yfjD"
                     /locus_tag="ACHOQW_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP9573313.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18518..19111)
                     /gene="grpE"
                     /locus_tag="ACHOQW_002238"
     CDS             complement(18518..19111)
                     /gene="grpE"
                     /locus_tag="ACHOQW_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9573314.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19234..20112
                     /gene="nadK"
                     /locus_tag="ACHOQW_002239"
     CDS             19234..20112
                     /gene="nadK"
                     /locus_tag="ACHOQW_002239"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP9573315.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
BASE COUNT         5034 a         5342 c         4720 g         5047 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat cattactgga
      121 cggcgacaat ccggtcttca ttaacttcca ggcgaataac tttacccgga accaattcac
      181 cagacagtat ttgctgtgcc aacgggtttt cgatctgctg ctggatagca cgtttcaaag
      241 gacgtgcacc atagactgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      301 aaatgtggat ttcataacca cgttcttcca gacgtttgta cagacgtttc aactgaatct
      361 gcgcaatcga ggcaatgtgc tgttcgccca gcggatggaa gaccaccact tcatcgatac
      421 ggttaatgaa ctccggacgg aagttatggc ttaccacacc gagcaccagc tctttcatgt
      481 gcgcataatc cagttcaccg aagcgttcct gaatcagatc ggaaccgagg ttagaggtca
      541 taatgacgac cgtattacgg aagtcgaccg ttctcccttg cccgtcagtc agacgcccat
      601 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
      661 gcaggatgac ggaatacgga cgacgacgca ccgcttcggt caggtagcca ccttcttcat
      721 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
      781 cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc gccagcgcct
      841 tacaaagctc tgttttcccc acaccagttg ggccgaggaa caggaatgaa ccaatcgggc
      901 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
      961 cttcgttctg accaattacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1021 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1081 tttcggcgtc ggtcacttta ttacgcaaca gacgcatagt tttgccttca agctgcgttg
     1141 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1201 ccaagtcacc cacacggcga gcctgttcaa tagcgatttt cgcctgttcc agttccgctt
     1261 taatggtctg cgtaccagaa agcgatgcct tctctgcttt ccactcttct tctaactcgg
     1321 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccagacgt tttttactgg
     1381 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacggcgat
     1441 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1501 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1561 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc aattcgtaac
     1621 gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggctcg gcaacaaaca
     1681 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     1741 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     1801 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     1861 acaggatgac gttgccttcc tgtttggcaa gatcgttaag cacgcctttt aaacgttctt
     1921 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtaccc
     1981 ggcggccttt caacccttcc ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2041 cgatggcagt tttaccgacg ccgggttcac caatcagtac cgggttattt ttagtacgac
     2101 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2161 tgccctgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2221 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2281 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2341 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2401 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2461 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2521 ttatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     2581 tcattaaatg aagtggttcg ataaattggt tgtcgtgccc aagtgcaagt gattgggcat
     2641 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     2701 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     2761 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     2821 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     2881 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgctcaaca cccacgttcg
     2941 ccagacgctg ccgggcaagc tgataaatgt ccgccagata cttatcacca tgctgaatga
     3001 aagctgtact cgcttcggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3061 caaacgcgcg tgggccaatt gccggcccta accaggcgag aatattttct ggattatcag
     3121 caaaacagga aactgtctct tccagcacgc ctgcgcacag tccacgccag ccagcatgag
     3181 cggcggcgac ttccgttccc gctcgattgc aaaacagcac agggaggcag tcggcagtca
     3241 tcactgcgca aaccctgccg ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3301 aaggttcgcc agtaagatta agcacatctt tgccgtgtac ctgctcaagc cagaccggtt
     3361 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg tgatccgggt
     3421 tatcgccaca atgggcaccg aggttgagcg agtcatacgg gggcaagctc acgccgccga
     3481 tacgagtaga gctacaggcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     3541 tactcataac cagtccactt catctttatg ctcttcgaaa tcggcgcgca tcacctcaat
     3601 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatac cggagatcgg
     3661 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaatttac gcagcgtgga
     3721 gataaacgct tccgaagcac cttttggcgg gcgcggacgg ccaccataaa ccggatcgcc
     3781 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccagtttc
     3841 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtcac
     3901 cgccggtttg cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     3961 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4021 agtaatttca cgcctttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4081 cacaaccatc aggccagtgg tgtctttatc cagacgatgg acgatgcctg cacgcggtac
     4141 atcagcaatg ggcggatagt aatgaagtaa cgcattcagt accgtgccat ccgggttacc
     4201 cgcgccagga tgtaccacca gatcgcgcgg tttattaatg acgatgatat cttcatcttc
     4261 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4321 gttgatggca acctgctcgc cacccaatac tttttctttc ggcttatcac aaagtttgcc
     4381 gttaaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4441 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4501 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4561 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     4621 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     4681 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     4741 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     4801 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     4861 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     4921 cgcaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     4981 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5041 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5101 ccaaactggt gcgcggctat ccaaacagtc agtacaccac cgatgccacc aaacgtctgg
     5161 tattcctgaa agatcgtctg gcgaaatatg aatactccgt ggccgagtac tatacagaac
     5221 gtggcgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata
     5281 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagatga
     5341 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5401 aacctgaaac acaaaacggc agcccttgag ctgccgtttg tttattctgt cagttgtgaa
     5461 actgaagcga tttagtcact atcgatctca tcaaatatgg ctcgctttga gatattcctc
     5521 aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa gattcgttga caaaaagtga
     5581 caaaattatg agatttccat cacacatttt gacatcagga acggtatgct gaattcacca
     5641 agacgggaag acaagaggta aaatttatga caatgaacat taccagcaaa caaatggaaa
     5701 ttactccggc catccgccaa catgtcgcag accgtctcgc caaactggaa aaatggcaaa
     5761 cacatctgat taatccacat atcattctgt ccaaagagcc acaagggttt gttgctgacg
     5821 ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg taaacatgaa gatatgtaca
     5881 ccgcaattaa cgaattgatc aacaagctgg aacggcagct caataaactg cagcacaaag
     5941 gcgaagcacg tcgtgccgca acatcggtga aagacgccaa cttcgtcgaa gaagttgaag
     6001 aagagtagtc ctttatattg agtgtatcgc caacgcgcct tcgggcgcgt tttttgttga
     6061 cagcgtgaaa acagtacggg tactgtacta aagtcactta aggaaacaaa catgaaacac
     6121 acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt ttcgtcgtgt
     6181 gaaacagaat gcgaagaaga acaataaggc ctcccaaatc ggggggcctt ttttattgat
     6241 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     6301 gcgcgctgga tgaaaaatta ttagcattac tggcagagcg gcgcgaactg gccgtcgagg
     6361 tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt
     6421 tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat tacattactc
     6481 gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg ctccaacaac
     6541 atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc aaaggttctt
     6601 attcccatct tgcggcgcgc cagtatgctg cccgtcactt tgagcaattc attgaaagtg
     6661 gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc gactatgccg
     6721 tcgtaccgat tgaaaatacc agctccggtg ccataaatga cgtttacgat ctgctgcaac
     6781 ataccagctt gtcgattgtt ggcgagatga cgttaactat cgaccattgt ttgttagtct
     6841 ccggcacgac tgatttatcc accatcaata cggtctacag ccatccgcag ccattccagc
     6901 aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc gaaagtacgt
     6961 ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg ttaggaagcg
     7021 aagctggcgg cactttgtac ggtttgcagg tactggagcg gattgaagcg aatcagcgac
     7081 aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat taacgtgtct gatcaggttc
     7141 cggcgaaaac gacgttgtta atggcgaccg ggcaacaagc cggtgcgctg gttgaagcgt
     7201 tgctggtact gcgcaaccac aatctgatta tgacccgtct ggaatcacgc ccgattcacg
     7261 gtaatccatg ggaagagatg ttttatctgg atattcaggc caatcttgaa tcagcggaaa
     7321 tgcaaaaagc attgaaagag ttaggggaaa tcacccgttc aatgaaggta ttgggctgtt
     7381 accctagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg
     7441 aatcatccgg cactggatta ttactggcga ttgtcattcg cctgacgcaa taacacgcgg
     7501 ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg
     7561 tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta
     7621 tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc ggcataaagc
     7681 tgcggatcct gagcgaacag tcgcccgacc atcgccagct caaggcggta aatcggcgaa
     7741 gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccaggtgcag cccgtaagca
     7801 aaagtagcaa agtggcgcag tgcctgaata aacgccatat tctgatcgtg ctcgacggcg
     7861 ctaatacgat gcaaccgagc gccccagacc tgaatttgct ccagaaacca ttggtatgct
     7921 tccggtttac gtccatcaca ccagaccaca acttgctttg ccaggctacc gctgtccgga
     7981 ccgaacattg ggtgtaaccc cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat
     8041 ggtccatttt tcactgatgc cagatcaacc agaatacaat ctttcggtaa aggcggtaat
     8101 ttgccaataa cttgctcagt aacgtggatt ggcacactaa caatcaccat tccggcatcg
     8161 gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac ctgataaccc
     8221 gagagtgtca gcatcttctc gaacagacgt cccatctgac cgccgccgcc aacgataact
     8281 accgggcgta acgcaggaca aagcgtttta aatcctttgt cgttttcact ggagtaagat
     8341 tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc cagagcttcc
     8401 gcctctgcac gacgcgaggc caacatagat gcctctcgct ccggaacata aataggcagt
     8461 ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt cgctaataaa
     8521 ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc
     8581 ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct gatgaatttc
     8641 acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg atacaccgta
     8701 tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat tactttcgat
     8761 catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg attctgccac
     8821 cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta ccatcagaga
     8881 cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg cagggctata
     8941 gttcggcgct ttaccaccgc gcaggatgac atgcccgtcc ggattcccct gagtttgtag
     9001 caacgcaacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg cggcggcgcg
     9061 catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaagc caaccggcat
     9121 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     9181 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     9241 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     9301 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     9361 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     9421 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     9481 agggccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     9541 acgcgagtca gcaatctggg cttcttgttg caggcttaat ggaaaagcgg ccttcagttg
     9601 tgccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttttg
     9661 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
     9721 acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttat ttacataagc
     9781 aatttattcg cgctttcaac ccttgattct gtatagataa atttacacca cctttgaaaa
     9841 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc gcccccttgt
     9901 tggctttact ggttagcgga tgccagattg atccttatac tcacgcgcca accttgacca
     9961 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc ataaaagatg
    10021 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
    10081 gacaaaagaa aacttgccag accgatttta cttatgcccg aggactttcc ggtaaaagct
    10141 ttcctgccag ctgcaacaat gttgaaaatg ccagccaact gcatgaagta tggcaaaaag
    10201 gggctgatga aaatgccagc gcaatacgat taaattaatt aaaaccccac aaataaatta
    10261 aacataagat tttactcatt atgttttatt tctcacaatg atgacgaccg catgaaataa
    10321 tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga
    10381 tccctgaatg cgtttttctc accgactgtt cttactgctg attctcctgc tgacgggagc
    10441 accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg
    10501 tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat gtatcttttc
    10561 atcttcacgt tttagtaccg ccctacaaga gaatgccgca acatctttgc cttatctgcc
    10621 tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt tttattttgg
    10681 caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa aggcgttatt
    10741 attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc tgatcatcca
    10801 caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg gtgtaaaggt
    10861 caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat aattctctta
    10921 ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc atatttatca
    10981 ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgcccctg aaacagtacg
    11041 cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa gcagctgtcg
    11101 tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag caagggcaat
    11161 tttcaactgc agaagtacgt gataagcagc aaaatattct ggcatcctgg cattacaccc
    11221 gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc cctgccccca
    11281 tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta accgctcgcg
    11341 acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt tgtattctgc
    11401 tggcatcagg aatcgcaatt accctcaccc gccatttgca caatggcctg gtggaagcac
    11461 tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt tcccgacgag
    11521 tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac agtctgctgg
    11581 atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt ctacgtaccg
    11641 cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc ggcatcaata
    11701 cgttgatgaa caattccgct gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
    11761 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagagtc ttgattgaaa
    11821 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
    11881 atgaattcgc tatggtgctc tatggtgtac agtcggaatc tgaagtgcag cagatatgct
    11941 cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat cagaccacca
    12001 tgacattaag cattggttac gcaatgacca ttgcgcacgc ttctgcggaa aaattacaag
    12061 agctggccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
    12121 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
    12181 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
    12241 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
    12301 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
    12361 cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac accgataact
    12421 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
    12481 catgggctat gggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
    12541 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc cgcgtcgcag
    12601 tggtgattac taccccttaa aacacgagca aacagataaa aaaagggcca gccaattggc
    12661 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
    12721 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
    12781 acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac gctcaacgcc
    12841 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
    12901 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
    12961 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
    13021 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg
    13081 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
    13141 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
    13201 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
    13261 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
    13321 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtact
    13381 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
    13441 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
    13501 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag ttcgctgacg
    13561 cccgcttgat caagcttgcg tccctgtggt gaaagataaa tcacctttgc gccttcaccc
    13621 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
    13681 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaaatcgcga
    13741 ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
    13801 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
    13861 tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
    13921 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
    13981 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattgga tccggtttcc
    14041 atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc
    14101 aggtctttcc agtagtagtc accctcttca agctgaggca gctgcgatga atccacgaca
    14161 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
    14221 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
    14281 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
    14341 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
    14401 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
    14461 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
    14521 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
    14581 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtcagcgaca
    14641 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
    14701 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
    14761 ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct
    14821 gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcatcccct tcatgcttct
    14881 catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca tgtcgtcgaa
    14941 ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag caatacggcg
    15001 tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca tcgagttgat
    15061 gatggcttcc atacgcacca gcactttatc gtccatctgt gacttgacgt tatccgggat
    15121 ctgccccatg cccggcagtt tgcccatcag gctggccatg ccgcccatat ttttcatctg
    15181 gcgcagttgc tcaaggaagt cgttgagatc gaagccgtca ccttttttca gcttgctggc
    15241 taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac
    15301 gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct ccagcgcctc
    15361 agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac gaatagagag
    15421 cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa
    15481 cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac
    15541 aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc
    15601 ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt
    15661 ggcttctttc agcgccgcgt taacaatatc aaccggcttc tgaccaacat cagaagggaa
    15721 gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata
    15781 gacgtcagca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga acttaccgag
    15841 tttaccaacg ctggttgttt taccggcacc ttgcaggcct gccatcagca cgaccgcagg
    15901 cggttgcgca gccagattca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt
    15961 acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac
    16021 cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga cgtccgcctc
    16081 cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag tgaggcgtcc
    16141 acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt
    16201 ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt attttgccgc
    16261 agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc
    16321 tatactgctt ctctttctta ttgctcaaac tgtcgacatc actatgcccg tttttgctct
    16381 gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa
    16441 aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc
    16501 aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct
    16561 gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg tggcttctcg
    16621 caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct
    16681 ggcaaccttc atgcccaatg aatacatcac ccatctggaa gctacgcctg ggatgctggt
    16741 gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct
    16801 gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcgttta accaggaaat
    16861 gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct
    16921 gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacttattta gcatggaaaa
    16981 tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctatgggg
    17041 acattatcat gaaggatggc gtggacgtcg cgtcgtctgg tttaacgttg cgggtgcagt
    17101 cattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca
    17161 gaaaaggagt ttccccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
    17221 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
    17281 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
    17341 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
    17401 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
    17461 aattgcgact ggtgtgctga cttttgtcgt actggttttt gctgaggtat tgccgaaaac
    17521 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctcccct
    17581 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
    17641 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
    17701 cactatcgtg cacgaatcgc gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
    17761 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
    17821 cggtattgat atcaacgatg actggaaatc tattctgcgc caactctccc actcacctca
    17881 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
    17941 tgaagcttgg cggttgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
    18001 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtgaagtt
    18061 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
    18121 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
    18181 aacacttgcc gaagaagtca cgccgcaaaa cgacggttcg gtgattattg atggcactgc
    18241 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
    18301 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgtaggca cccgcgtgcg
    18361 tattggcgag tacgatatcg atattctcga cgtacaggac aatatgatta agcaggtaaa
    18421 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgaaaac ggcccggcat
    18481 tcgcatgcag ggccgtgaat tattacgaaa acagaaatta cgcttttgct ttcgctacag
    18541 ttaccatcgc cgcacgaatc gtacgaccat tcagcgtata acccttctgc ataatgccca
    18601 gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatagcctga tgcacattcg
    18661 ggtccagcgg cacgttagtt tcggcgatca cttcaacacc aaacttacgc acaacatcca
    18721 gcatcgactt cagcgtcagc tcaatgcctt caaccatcgc agacatatcc gggttagctt
    18781 tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga
    18841 atttctccag cgcgaatttg tgggcttttt caatatccag ttcagtacga cgacgcaggt
    18901 tttccatttc ggcttttaca cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca
    18961 gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt
    19021 ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc
    19081 cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat
    19141 ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt
    19201 gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc
    19261 attgtgggac acccacggca ccctactgca ctgacaacac atgaaatgct ctaccgctgg
    19321 ctgtgcacaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg
    19381 aagaatgtga aaactggcac gctcgcggag attgggcaac tggctgatct cgcggtagtc
    19441 gttggtggcg acggtaatat gctgggcgcg acgcgcacgc tcgcccgtta cgatattaaa
    19501 gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc cgataacgcc
    19561 cagcaacaat tagccgatgt gctggaaggt cactacatca gcgagaaacg ttttttgctg
    19621 gaagcgcaag tctgtcagca agattgccaa aagcgcatca gcaccgcgat aaatgaagtg
    19681 gtacttcatc caggcaaagt ggcgcatatg atagagttcg aagtgtatat cgacgagatc
    19741 tttgcgtttt ctcagcgatc tgatgggcta attatttcga cgccaacagg ctccaccgcc
    19801 tattccctct ctgcgggcgg tcctattctg accccctctc tggatgcgat taccctggtg
    19861 cccatgttcc cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc
    19921 cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca
    19981 ctaccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg
    20041 attcatccga aagattacag ttatttcaac acattaagca ccaagctcgg ctggtcaaaa
    20101 aaattattct aattttacgc cagcctcttt actgtatata aaa
//
LOCUS       DAZKFZ010000065        20060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000065 DAZKFZ010000000
VERSION     DAZKFZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(125..871)
                     /gene="cutC"
                     /locus_tag="ACHOQW_002240"
     CDS             complement(125..871)
                     /gene="cutC"
                     /locus_tag="ACHOQW_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9573316.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(885..1451)
                     /gene="yecM"
                     /locus_tag="ACHOQW_002241"
     CDS             complement(885..1451)
                     /gene="yecM"
                     /locus_tag="ACHOQW_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9573317.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1667..3400
                     /gene="argS"
                     /locus_tag="ACHOQW_002242"
     CDS             1667..3400
                     /gene="argS"
                     /locus_tag="ACHOQW_002242"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9573318.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3442..3486)
                     /locus_tag="ACHOQW_002243"
     CDS             complement(3442..3486)
                     /locus_tag="ACHOQW_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573319.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3577..4065
                     /gene="yecT"
                     /locus_tag="ACHOQW_002244"
     CDS             3577..4065
                     /gene="yecT"
                     /locus_tag="ACHOQW_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP9573320.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRTWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4185..4577)
                     /gene="flhe"
                     /locus_tag="ACHOQW_002245"
     CDS             complement(4185..4577)
                     /gene="flhe"
                     /locus_tag="ACHOQW_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP9573321.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4577..6655)
                     /gene="flhA"
                     /locus_tag="ACHOQW_002246"
     CDS             complement(4577..6655)
                     /gene="flhA"
                     /locus_tag="ACHOQW_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP9573322.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6648..7796)
                     /gene="flhB"
                     /locus_tag="ACHOQW_002247"
     CDS             complement(6648..7796)
                     /gene="flhB"
                     /locus_tag="ACHOQW_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP9573323.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7998..8642)
                     /gene="cheZ"
                     /locus_tag="ACHOQW_002248"
     CDS             complement(7998..8642)
                     /gene="cheZ"
                     /locus_tag="ACHOQW_002248"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP9573324.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8653..9042)
                     /gene="cheY"
                     /locus_tag="ACHOQW_002249"
     CDS             complement(8653..9042)
                     /gene="cheY"
                     /locus_tag="ACHOQW_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP9573325.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9057..10106)
                     /gene="cheB"
                     /locus_tag="ACHOQW_002250"
     CDS             complement(9057..10106)
                     /gene="cheB"
                     /locus_tag="ACHOQW_002250"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP9573326.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10109..10969)
                     /gene="cheR"
                     /locus_tag="ACHOQW_002251"
     CDS             complement(10109..10969)
                     /gene="cheR"
                     /locus_tag="ACHOQW_002251"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP9573327.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10988..12589)
                     /gene="tap"
                     /locus_tag="ACHOQW_002252"
     CDS             complement(10988..12589)
                     /gene="tap"
                     /locus_tag="ACHOQW_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP9573328.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12635..14296)
                     /gene="tar"
                     /locus_tag="ACHOQW_002253"
     CDS             complement(12635..14296)
                     /gene="tar"
                     /locus_tag="ACHOQW_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP9573329.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14439..14942)
                     /gene="cheW"
                     /locus_tag="ACHOQW_002254"
     CDS             complement(14439..14942)
                     /gene="cheW"
                     /locus_tag="ACHOQW_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP9573330.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14963..16927)
                     /gene="cheA"
                     /locus_tag="ACHOQW_002255"
     CDS             complement(14963..16927)
                     /gene="cheA"
                     /locus_tag="ACHOQW_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP9573331.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16932..17858)
                     /gene="motB"
                     /locus_tag="ACHOQW_002256"
     CDS             complement(16932..17858)
                     /gene="motB"
                     /locus_tag="ACHOQW_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP9573332.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVCVPTMPSAEPR"
     gene            complement(17855..18742)
                     /gene="motA"
                     /locus_tag="ACHOQW_002257"
     CDS             complement(17855..18742)
                     /gene="motA"
                     /locus_tag="ACHOQW_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP9573333.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18869..19447)
                     /gene="flhC"
                     /locus_tag="ACHOQW_002258"
     CDS             complement(18869..19447)
                     /gene="flhC"
                     /locus_tag="ACHOQW_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP9573334.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19450..19800)
                     /gene="flhD"
                     /locus_tag="ACHOQW_002259"
     CDS             complement(19450..19800)
                     /gene="flhD"
                     /locus_tag="ACHOQW_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP9573335.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4798 a         5529 c         4894 g         4839 t
ORIGIN      
        1 tcaacagatc gcggtaatcg ttcatttttt atactattca gggataaaaa aaggcccctg
       61 ttgaaattgc aggggcctgg tacgagcaag catcatattg ggcgacatga tgcaacggta
      121 aaaatcattt ggcctgatgg cgttcaatga ttcctttcat ttcagcaacc gccgccccgt
      181 ctacgacata acgcgaatac tcgtccgcgt gtgcatctga tgacatggac aatccttgat
      241 tacgataacg catcggtgag gcttgccacg ctcccgcgga gctatggact tccagcactc
      301 cggcatcgag gaagtggtgc aggttttctg cacggactcc tgctccggcc ataatgattg
      361 gagcatcacg atgggcaata agttccataa tttttgataa accttgcagt gcgtctgatt
      421 tttgccctga tgtcagtacc cgggcaatgc ccaattccgc aagattattg agtgtattta
      481 gagggttagc gcacatatca aaggcgcgat gaaaagtcac tgccagcgga ccggcagcag
      541 ccattatttt ttccattcgt gacatatcga cattaccgtc aacatcgagc acgcccgtca
      601 cgagtccagg aaaacctaat tcgcggaccg tgcgcacatc ctcaagaatg gcggcaaact
      661 caccgtcgct gtagcaaaaa tcaccaccgc gtgggcgaat tatcggatgc acagggatcg
      721 tcacccgctg gcgcacggat ttcagtacac ccaacgacgg cgttaagccc ccctcttttg
      781 gggctgcgca taattcaact ctgtctgcgc cgttttgctg cgccgttagc gcgcattcca
      841 tgctgtaaca gcaaatttcc agtaatgcca tttttgctcc ttaattacgc cgactgctcg
      901 ctggcaacaa tctcttcaat ggaccacgga tgaaatttaa tggtcgtttt gccatcggtg
      961 accgccagcg tcggattcgg taatcgttca tgctcacctt ttggcgaact ggttttaact
     1021 gagatccccg gtaggctgag tccttcatca gaaagcaatg ccagcgcacg ggcattcagc
     1081 gtttctggat cacccggcaa aacgatttca atatgttccc aaccttcgtg cgggtaacgt
     1141 ttttccctag gccacggtag ctccacaata gaaaactgcc agtgtgcaac ctgtaccggt
     1201 tcatgcaatt taaacagaca aatcggtctg ccattgatca tattttctga caaaagctcg
     1261 ccacactgtt caaacccgcg acgccagcgc tcagcagtgg cgttttgatg gcaacgcaaa
     1321 gaaatgtgat cggcagtcag cggagtgata ttcaacccca gacggcggga aagttcatct
     1381 aatgcgtggg taaatcgcgg taaatccgat gcaatatcct gcagctcgtc gatagattgc
     1441 cagttcgcca taatcactct tcgtctttca gtaaaagcgt taatttaccc tgttgccctg
     1501 cgccaaccaa ccgctgattt cacgccgctt ctgatgcaat agtgaaaacg gcaatacgcc
     1561 acgcgcacgt tgctgacgaa aacagccatt tgcagtatac tcccgcccta atttctttaa
     1621 ctggtgcggg caatttttgc tcgcttcatc aatgtaaggt attccggtga atattcaggc
     1681 tcttctctca gaaaaagtcc gtcaggccat gattgcggca ggcgcgcctg cggattgcga
     1741 accgcaggtt cgtcagtcag caaaagttca gttcggcgac tatcaggcta acggcatgat
     1801 ggcagttgct aaaaaactgg gtatggcacc gcgacaatta gcagagcagg tgctgactca
     1861 tctggatctt aacggtatcg ccagcaaagt tgagatcgcc ggtccaggct ttatcaacat
     1921 tttccttgat ccggcattcc tggctgaaca tgttcagcag gcgctggcgt ccgatcgtct
     1981 cggtgttgct acgccagaaa aacagaccat tgtggttgac tactctgcgc caaacgtggc
     2041 gaaagagatg cacgtcggtc acctgcgctc taccattatt ggtgacgcag cagtgcgtac
     2101 tctggagttc ctcggtcaca aagtgattcg cgcaaaccac gtcggcgact ggggcactca
     2161 gttcggtatg ctgattgcat ggctggaaaa gcagcagcag gaaaacgccg gtgaaatgga
     2221 gctggctgac cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga
     2281 gtttgccgag cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg
     2341 cgagatgtgg cgcaaactgg tcgacatcac catgacgcag aaccagatca cttacgatcg
     2401 tctcaacgtg acgctgaccc atgatgacgt gatgggcgaa agcctctaca acccaatgct
     2461 gccaggaatt gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac
     2521 cgtcgtattc cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca
     2581 gaagaaagat ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta
     2641 tgaaacactg catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct
     2701 gatgcaggca tgggctatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga
     2761 acaccacatg ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg
     2821 tggtacagtg aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt
     2881 ggcagaaaag aacccggata tgccagccga cgagctggaa aaactggcta acgcggttgg
     2941 tattggtgcg gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga
     3001 ctgggacaac atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcatacac
     3061 gcgtgtattg tccgtgttcc gtaaagcaga aattgacgaa gagcaactgg ctgcagctcc
     3121 ggttatcatc cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga
     3181 aaccctcacc gtggttgccc gtgaaggcac gccgcatgta atgtgtgctt acctgtacga
     3241 tctggccggt ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga
     3301 agaagtgcgt aacagccgtc tgaaactggc acaactgaca gcgaagacgc tgaagctggg
     3361 tctggatacg ctgggtattg agactgtaga gcgtatgtaa tcgatttttc gtgagagtga
     3421 agcctgatca ggtttagccg atcaggcttt tttattacca tctaaatgta ttctgaaaat
     3481 aggcatgcca ttgttttctc gctgttgagt aagaggccag aagcgtaata tccagctcca
     3541 gggaaacggt aacggttcaa tttaaggaat accgcagtgt ttaaatttct tgtattaaca
     3601 ttaggcatta tctcttgcca ggtttacgca gaagatacgc ttatagtaaa tgatcatgac
     3661 atttcagcca tcaaagattg ttggcaaaaa aattcaggtg atgatactga cattaacgtg
     3721 atcaaatcat gtctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcttat
     3781 ggtgaagctt atcgttatat agaacaagtg ccacgcacag gtgcaaaaaa acctgatact
     3841 gaacaactta acttgcttaa aaaatcacaa cgaacctggc tggattttag ggacaaagaa
     3901 tgtgaattaa tcctttcaaa tgaggacgtt caggatttaa gtaaccctta ttctgaatca
     3961 gaatggctat catgtatgat catacagacc aatacgcgaa ctcgccagtt gcagctatac
     4021 cgtaactctg aagattttta tccaagccct ttgacaagag gataattcac atatttttgg
     4081 catgttttgt tgcaagctat tcctgataaa tgattgcaac aaaacatcca gcctttttca
     4141 ctgagttatt aaacatactc gcgagcgcgt aatttttttg tccttcagcg ataattcaca
     4201 atcacttcat tacgttgtac tttcagcgac ggaattaacc gcccaccgcc aggcacttcc
     4261 cagataaagc gcaacggttc tgctgccgct ataccggaaa aggccacggt ggttccgctc
     4321 tgcccatcta attcgacaca acgagattgc gaacacaagc gaacccgcag tcctgcaggt
     4381 gtcgggccga taagctgata ccgccacgcc accagcgtca tcaatcctga agcaggttgt
     4441 cgcgtagaaa gaggtgcaga cgacatcgac tcaccgcgat gatttagcgt aattccgaca
     4501 ctacttgcct gccacatccc ctccccggcg gcttgcacca gcagcggaaa caataatatt
     4561 gctaataagg ttctcatcat ttaccgccaa ttgtcgccgt catgcggata tgtcggttat
     4621 cagacagttc cagattcgac agcaccacta actgcggcaa gctgcggcgc agaaagcgag
     4681 acaataatgg tcgcagcgcg tggttcacca ataaaactgg cggcgcaccc aacatctcct
     4741 gacgggatag cgcttcctga gtttgcgcca gtaaacgatc cgccagccct ggctccagtc
     4801 cgcccccgcc ctgcaacgcc tgtagcaaca aacgttccag cggtgtatcg aggccaataa
     4861 catggacttc atctttgcca ggaaaccact gctgggtaat cgcccgtccc aacgccacgc
     4921 gcacgacggc ggttaattca tgtggatcgc tttggatggg cgcatgttcc gccagcgttt
     4981 cgagaatagt gcgcatatcg cgaatcggta ctttttcatc gaggagattt tgcagcactt
     5041 tatgcagtgt ggtgagcgtg acgacgccag ggacgagatc ttccgtcagc tttggcatct
     5101 cctgggcgac gcgatccagc agctgttgcg cctcctgacg accaaacagc tctgcggcat
     5161 gctggctaat gaggtggtta agatgcgttg ctaccaccgt gctggcctca accactgtgt
     5221 acccctgaat ctgcgcctgt tcttttagcg cactttcaat ccagatagca ttcaggccaa
     5281 atgccggatc gacggtcgcc tcaccaggta acgtcccggc agcggttcca gggttaatag
     5341 ccagccagcg ccccggataa gcatcaccac tgcctatctc cacgcctttc atcaaaatgc
     5401 gatagcgggc aggttgcaga tccatattgt cgcgaatgtg caccactggc ggcagaaatc
     5461 ccatctcctg ggcaaatttc ttgcggatac tgcgtatacg gcccaacaac tcaccatcct
     5521 gctggaaatc gaccatcggg atcagtcgat aacccacttc cattcccaga gaatcttcca
     5581 gttgtacatc gttccacgtc gcttcgacaa cggcattatt ctctgccatt tttaccggtt
     5641 ttggttcggc aggcgctttt tgttcgcgtc cacggatcca ccaggccagc cccagcaatc
     5701 cggcagtgaa cagcaaaaat accaggttcg gcattccagg caccaggccg agtaaaccga
     5761 gcacggcagc gcttaacaac ataacgcttg ggttactgaa aagctgattc accatctgct
     5821 cgccaacatc ctgatcggtg ctgacacgcg taacaatgac ccccgcggcg gtagaaatca
     5881 ccagagccgg gatttgtgcc accagaccgt caccaatggt caagagcgta taactttccg
     5941 ccgcgtgtcc catgctcatg ccatgttgca gcacgccgac cagcaacccg ccgacaacgt
     6001 taatgaccat gatgaggatc ccggcgatgg catcgccgcg aacaaactta cttgccccgt
     6061 ccattgagcc gtaaaaatcg gcttcctgag tgacttcgga gcggcgtttt ttcgcctcat
     6121 cttcaccaat caatccggcg ttaaggtcgg cgtcaatcgc catctgctta cccggcatac
     6181 catcgagaac aaagcgcgca cccacttctg cgatacgccc ggcacctttg gtaatgacca
     6241 taaagttgat gatcacgaga atgacaaaca ccacgatacc gatagcgaaa ttgccaccaa
     6301 cgaggaagtg accgaacgct tcaaccacct tccctgccgc cgctgcgccg gtatgccctt
     6361 ccattaaaat gatacgggtt gaagccacgt taagtgccag acgcaacagc gtggtaaaca
     6421 acaaaatggt cggaaacgca gcaaactcaa gcgtgcgctg ggtaaacatc gccaccagca
     6481 acaccatgat cgacaaggca atattgaagg taaacaacag gtcgagtatg aatgcgggca
     6541 gtggcagcac catcatcgac aagatcaaca ggatcaaaat cggtccggca aggatctgcc
     6601 attgtgtcga tttcaggttt gtgggcaggc gcagcatcgc ggccagatta ctcatgggtc
     6661 ggtttctcgt taataaaatc cagggcttcc ggcaccggaa gatgagtagg ttgtacaggg
     6721 cgctgtccac cagccagacg ccagcgtttc agttgccaga cccaggccag cacttccgcc
     6781 accgcggcgt acagttgacc cgggatttgt tgaccaatct ccacatgtcg atacagcgct
     6841 cgcgccagcg gcggcgcttc aagcgtcggg acgttatttt cagcgccaat ttcacgaatg
     6901 cgcagcgcga ccagccctgc acctttagcg accactttcg gtgcgctcat tttgttttcg
     6961 tcatactgca acgctaccga atagtgggtc gggttattga caatgacatc cgctttcggc
     7021 acatcggcca tcatccgacg ccgtgcggca gctcgctgca tctgacggat ccgcccttta
     7081 acatgggggt cgccttcgct ttgtttgaac tcatcacgaa tatcctgccg cgacattcgc
     7141 agctttttca ggtggctgaa gatttggaaa aagacgtcaa atcccaccat tggaatgaca
     7201 ccaagcacca ccagcagtgc gcatagccct accaaatcca tcgcattacc catggcggta
     7261 atcggagact cggccattaa gcgcatcata tgcggccagt gatgccagag aaaaaacccc
     7321 gtcacgctgc caaccaggat ggttttcaaa attgctttaa gcaactccgc gccagtctgt
     7381 gccgagaaca tccgttttat gcccggtagc gggttgagtt tggaaaactt cggctgcaag
     7441 gattttccgc taaataccag cccccccagc ataaccggag aaatgagcgc caccagcacc
     7501 acgccgctaa tcagcggtag cagcgccagc atggcttctc tgatcagcag aataatttgc
     7561 ccgaggatca gattcgggtc tttgataata ctgtgatcaa aatgcagccc agcggagagc
     7621 atgcccgaca atcgacgggc cagcgacaca ccgccaaacc agataacact aacgcccacc
     7681 agcaaaatca gcagtgaggt cagttcacgg gaacgcggga tttgcccctc ttcccgcgct
     7741 ttttctagtc ggtgaggtgt gggggcttct gttttgtcgt cgctctcgtc agacacgtcg
     7801 ccaatcctgg aaagagtatt aagcgtaaat gatgccagag cgcaaagcgt tcaatggttt
     7861 gagtaagggg caaaacaggc gggatttagg gcttttgctg ccacacatca agcatagtgt
     7921 gcgtttgtca gatgcggcgt catcgcctta tcagaccgcc tgatatgacg tggtcacgcc
     7981 acatcaggca atacaaatca aaatccaaga ctatccaaca aatcgtccac ctgatcctga
     8041 ctggctacca caccggcttt gctggtatcg acctgaggtc cattaagcaa actctggttt
     8101 tcacgttttg gacgcgactc ctgttccggg atgttttcca acagcaccat cagcaactgg
     8161 cgttcgatct cctgaatgac atccatcatc cgcttaatga cctgcccggt gagatcctga
     8221 aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag taaagctggt atgcgcgggt
     8281 acatctgcca gaaatttccg cgtatctgtt accagttcac gggcgtcggc aaggtcaatc
     8341 ggatcggcaa accagtcatc ccaacgttgg gttaacgctt ttgctgattt ctccatttga
     8401 tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc gctccgcagc ctgggcggtc
     8461 atctgcacaa catagtacaa acgatcgcgc gcatcgggga tggcttccgc cgcttcggca
     8521 atggcctgat ccagtcccag ttcccgcaaa ctgtcgcgca gcatacgcgt caggctgccg
     8581 atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag gtttgattga tggttgcatc
     8641 atagtcgcat cctcacatgc ccagtttctc aaagattttg ttgagttttt cctccagcgt
     8701 cgcggcggta aatggcttca ccacatagcc actggccccc gcttgcgccg cagcaatgat
     8761 gttctctttc ttcgcttctg cagtcaccat taacactggc aatgccgaca tcgcgccatc
     8821 cgcacgaatc gttttcagca attccaggcc atccatgttg ggcatgttcc agtcggagat
     8881 aacaaatcca taaccgcctg cctgcaactt attgagagcg tcgacgccat cttccgcttc
     8941 ctcaacatta ttgaatccca gctctttcag caggttacgc actatgcgtc gcatggtgga
     9001 aaagtcatcc acaaccaaaa atttaagttc tttatccgcc atttcacact cctgatttaa
     9061 atacgtatcg cctgtccggc actaattttt gccaacattt gctggcttac ctggttaaga
     9121 tcgaccactt cgcagacacc acccatattg atggcctcgc gcggcatgcc gaacaccacg
     9181 caacttgctt cgttttgcgc aagggtccat gccccagcct gacgcatcgc caacattccc
     9241 gccgcgccgt cgttgcccat accggtcagg atcaccccaa ccgcattacg ccccgcctgt
     9301 ttggcgacag aatggaacaa cacatctacc gaaggccgat gacggttaac cgccgggcca
     9361 tcgtgaattt tgatttggta atttgcgcca ctacgcgcca gctccatatg ccgatcgccc
     9421 ggcgcaatat aggcatgccc cggcaaaaca cgttccccgt cttcggcttc tttaaccccg
     9481 atctggcaaa gcttattaag tctgtcggca aaagagcggg tgaaaccggg cggcatatgc
     9541 tgggtaatta acagtgccgg gctggaaagc ggcaacggtt gcagtacgtg acgaattgcc
     9601 tcagttccac ccgttgaagc accaatcgca atcagttttt cagaactcaa caacggccct
     9661 gccttcagcg ttgtcggtgc cgacaatggc ttatgtgctg caaggctcgc ctttgctgct
     9721 gtacgcacct tttcagcaat catttcgctg tacgccagca ttccttcgcg aatacccagt
     9781 tgcggtttgg tgacaaaatc tattgccccc agctccagcg cgcgcagcgt gacttctgat
     9841 cctttgccgg tcagggaaga aaccatcaca acgggcattg gacgcaaacg cattaatttt
     9901 tcgaggaaat ccagtccgtc catccgcggc atttcaacat ccagcgtcag cacatcggga
     9961 ttgaatttct taatcaagtc acgcgcgacc agcggatcag gcgcggtcgc caccatttcc
    10021 atgtcgctat ggctgttgat gatttctgtc atgatctggc gcatcagtgc cgaatcatcg
    10081 acagataaca ccctgatttt gctcatcgtt aatccttact tagcgcatac accgtttgac
    10141 cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc agagtgaccc gcaaacagca
    10201 atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat ctcctgctgg gtagtttgat
    10261 cgaagtagat catgacgtta cgacagaaga tcgcatcaaa cggccccggc acggtgtact
    10321 gtttcgccag tagattcagc gaggcaaaat caacatagtt cgccagctcc tgacgcacgc
    10381 gtaccagccc ttcatgcggt cccgtccctc gcatgaaata ccgttgcagt tgctgcggcg
    10441 tcaggttttt caactcttca tggcgataga taccgcttct ggctttttcc agcacttcgg
    10501 tgtcgatatc actggcaaac actttccagc gtccgggcgc ggtgcccaat gtgtcagcca
    10561 gcgtcatcgc aatgctgtac ggctcttcgc cggtcgaagc cgccgcgctc catacgcgat
    10621 actcgccaga acggcgacgt gcgtgatccg cgagcagagg gaaatgatgt gcctcacgga
    10681 aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc ctgccactca ccgctgtgct
    10741 gattagattc cagcaagttc agataatgac cgaaatccgt cagtcccagc gaacgcaaac
    10801 gacgaaccag tcggttgtaa accatgtcgc gtttatggtc agccagaacg atcccggctc
    10861 gttgatagat caattgactt atccgccgaa aatgcgcgtc ggaaagcgcc aggcgctcgg
    10921 tcatctgtaa caataaagac gtttgcccac agggcagaga tgaagtcata gcgccttctc
    10981 aatcacttca ggataccact ggcgcaattt gtaactgcgc cgactcatgt cgtgctactt
    11041 catgttcttc aagggtaaat accgccacac gcgacgaaag atggtcggcc tgattcgcca
    11101 gttgttccgt tgccaccgcc gcttcttcta ccagcgaggc gttctgctgt gtcacctgat
    11161 ccatctggct gacagcctgc gcaacctgct caatcccccg ccgttgttct tccgaggccg
    11221 aagcaatttc tcccataatg tcgttcacgc gggtcaccga actgacaata tcggtcatgg
    11281 tcgcggcggc gttattcacc agtttcgaac cctgctggac acgattgact gactcttcga
    11341 tcagcccttt gatctctttt gccgcctggg cgcttcggct ggcaagattg cgcacttctc
    11401 ctgccactac cgcaaaacca cgcccctgct ctccggcgcg agccgcttct actgccgcat
    11461 tcagggccag aatattggtc tggaaagcaa ttccgtcgat aacgctgata atgtcgccaa
    11521 ttttttgcga gctggtggcg atctcctgca tggtgtgagt catggtactg acctgaacac
    11581 cgcccgcctg cgccgttgtc gcggcatttt ttgcaagttc cgacgcctgt cgtgcgttat
    11641 cggcgttttg ccctaccgtg gcggttaatt gctccatact ggcggccgtt tgtgccagcg
    11701 atgccgcctg ctgttcggta cgacttgaga gatcgttatt gcctgcgaca atctccgcga
    11761 taccaatgtg catctcctgg cttcccttac gcacatcact taccgtccca cgcaaagcct
    11821 gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc attacgacca tataccgcaa
    11881 tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg gctaccgata atggccagtg
    11941 gttgaacaat catcttgcgc gtccaccaca gcgcactgct gatgtagatc gccgcaacaa
    12001 taatcatgct gataaacacc agcgccgaaa tctgatagtt acgctggctt tgcgcactgg
    12061 cggcttccag cacatggatg atctccagct gccaggcctc aaagttaacg tcaaacgcat
    12121 tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag ttgattactt tcaagccagg
    12181 tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact tttctccgtc tctttttgca
    12241 atgccctgac gtgctcgttg cccgcagtca tcgccataaa acttttaaac agcgtggtgg
    12301 attgcgtcag actggcacgc gccgtcgtca tcaacgtttt aatgtcatcc gccggatagc
    12361 taagcgctgt cagagtgcct gctttgttca gcgtggtact ggcctgtaac attaccgccc
    12421 gagtttgcgc taatgccgca cgttgctgat tgctctgctc gacctgattc agtcgttgca
    12481 aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc aatctgcaag atcccgcaga
    12541 gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg attaaacatc aacgctcccc
    12601 atcaggcggc aatgaccgcg ttagtaaata ctcgtcaaaa tgtttcccag tttggatctt
    12661 gttcagcaat tcgcagtcgt ggctgtgccg gtggttgctc actggcagga cgggatggtg
    12721 tttgcggttt attggtgagt gggctggctg ccagacgaaa cgcggaaacc gcttgcgtta
    12781 aacgactagc ctgttcttcc agcgcagctg cagcggcagc tgattcctgc accagcgatg
    12841 cgttctgttg cgtgacgtga tccatttccg aaaccgccaa tgcgacttga tcaatgccac
    12901 ggctctgttc atccgatgcc gatgcaatct cgcccataat gtcagtcacg cgagtgacag
    12961 catcgacgat attgttcatt gtttccccgg cgctttcgac cagcaccgaa ccggtatcaa
    13021 cgcgcgagac ggagtcttca atgagagctt tgatctcttt tgccgcctgg gcgctacgac
    13081 tggcaagatt acgcacttcg cccgccacca cggcaaaacc acggccctgt tcacccgctc
    13141 gcgcggcttc aacagcggca ttcagcgcga ggatattagt ctggaaggca ataccgtcga
    13201 ttacgctgat aatgtcggcg attttcttcg aactatcggc gatctcatgc atcgttttca
    13261 ctacgccatc caccactttg ccgccgtgct gggcggtgtc ggaggcactt tgcgccagtt
    13321 gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt cgcggtgagc tgctccatgc
    13381 tggcggcagt ttcttccagc gcggatgcct gctgttccgt acgggaggaa agatcggtgt
    13441 tgcccgccgc aatttcacgg gtaccggcat agatggcatc tgaaccttcg cggacatgag
    13501 tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct ctgcgccagg tcgcccattt
    13561 cactgcgccc gtcaatggtc agggtattcg ccaggttacc accggcgatt tcgcgaatgt
    13621 gagcaataat ttttgccagc ggtgtaagca acatacggcg aatgccatac cacgccacca
    13681 gcagaatcaa taccaccacc agcgcgataa ccgccagttg ccactgggca aatcggtaat
    13741 catctgcgtt gtcagtgacg atatcgcgat acagtttttc actgctgagg gcgtactgag
    13801 caaacgcttc gcccattgca ttttgcattc cctgggttgg ctgagcgaaa taagctccag
    13861 tattgccata atcaagataa tcaatcagtt cagttaacgc tgtgtaatag tttttatatt
    13921 tttcatcaat attacgactg gtagcgacca tttcaggtaa cggtgccatg cttttgaatt
    13981 ttttataatg agtcgcggcc tgggccaatg ttttcctggc gctatcgagc aattcaactt
    14041 tggcattact ttgttgattg gaggaatcca tcatcattcg caccgctgaa cgactcaggt
    14101 taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag ctcgccctgc tgttctcgta
    14161 attgattgga aaccacaaag ctcttctggc tatggtgaag ggaagaaaaa aacagactgc
    14221 cggaaataag ctgtaacagt gcgaataccc ccagcaccat taccaacagc gtgactacgc
    14281 ggatacggtt aatcataagg caccttcctg aaaacaagtt gatctcgtta tcggcaagga
    14341 agggggaaac tttattgctg atgccacccg ccgcgaaatt gaaataaaac ccgatgcgca
    14401 gatcatcggg ttcatttcaa ttgaggaaat caggagaatt acgccacttc tgacgccgcg
    14461 ctatctaaca gcgccatctc ttcgctgttc agcagttttt cgatgttcac cagaatcaac
    14521 atacggtcgc ccagtgcgcc cagtccagtg agatattctg ttgaaagcgt cacggcaaac
    14581 tctggtgccg gacgaatttg ctccgccgtc aatgaaagca cgtctgaaac gccgtcaacc
    14641 acgatgccga ccacccgctg tccgagattc aggacgataa ctaccgtgtt gtcgttatag
    14701 tccacatcca cctggctgaa cttaattcgt aagtcaacaa tcggcacaat aacgccgcgc
    14761 agattcgtga cgcctttgat aaacgctggc gtgttcgcaa tccgtgttac ctgatcgtag
    14821 ccacggatct cctgcacttt caggatatca ataccgtact cttcatcacc aagggtaaat
    14881 accagaaatt cctggcctga cggctcgctg gccagctttg ttacattcgt cataccggtc
    14941 atattgttac ctttttactc attcaggcgg cggtgttcgc catacgttgt tcgcggttta
    15001 tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct gccgtcgcca agaatggtcg
    15061 cagcagaaat gccggggact ttgcgatagt tactttcaag gtttttaact acaacctgat
    15121 gttgaccaat taattgatcc accagcaagg cgtagcggcg accgccactt tgtaagatca
    15181 ccacaattcc ctgggtagct tcggttttcg cgcccgcgac gttgaacact ttccacagtt
    15241 cgacgatggg cagatattca ccccgcactt ccagcacccg ctcgccgccg gccagtggat
    15301 gcagatcggc ttcacggggt tgcagtgatt ccataacagc attcagcggc agaatgaata
    15361 cttcatccgc aacgcgtacg gacatgccgt cgaggatggc cagcgtcagc ggcagtaaaa
    15421 tgcggatcgt agtgccagta ccctgcttcg actggatttc aacatgaccg cccatctcct
    15481 ggatattacg tttaacgacg tccatgccga cgccgcgccc ggagacgtcg gtgacctgct
    15541 ctgccgtgga gaagcctggt gcaaatatca gcatcgcgac ttcgtcgtcg ctcatgtttt
    15601 cgctgacagt caaaccttgc gaggccgctt ttgccagaat tcgctcacgg tttagccccg
    15661 ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg atgttcggca gacagaatta
    15721 aatttccgac gctgttttta cctgcggcga gccgtttttc tggcagttca ataccgtgat
    15781 cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat gcgttctatc aggctcttgt
    15841 cgagctcggt ggaactgccc accagcgtca gttctacctg cttgccgagt tttcccgcca
    15901 gatcacgcac cagccgggga tagcgactaa agacatattc catcggcatc atgcgaatcg
    15961 acatcactga ttcctgcaaa tcacgggcgt tacgttgtaa ctgccccatg ctggtaatca
    16021 aatcaccatg attaaccggg tccagttcgc tggaacgctg ggcaagcatg gactgggtga
    16081 taaccagctc gccgacgagg ttaattaatt gatcaacctt ttctaccgct acacggatgc
    16141 tggtggattc actgctacgc gtcgtttttt cccgctccac gcgaccggtt ggcgcttgtt
    16201 cggctgccag tttaagcact ggtggggtgg atatttttgg cgagacttct actgtttcaa
    16261 aggtaatctg atcggcttca atcacaaaac agagtaccgc tgtgatgtca tcttcggcga
    16321 tatcgcccgg taatattgcc gagagagaat ccgccccttt caccacgtca gttaacgttg
    16381 tcagatgccc cagctcttct tccagcaggt cgacttcgct ggccttcagg cgcgaaagga
    16441 taattcgtcg cggcaactga ctgcgactct gctcatcttg cggttcactt ttggcaacca
    16501 cacttaatcg ggtcactgcg gatggcgttt cgcctttcgc ttctaatgcc agttgacgca
    16561 aggcctggca gatataatcg aagctggcgg catccggctc ttgcgactgt ttataagcgt
    16621 cgagctgttc ttgcatgatg tccttcgttt ccaaaaacag attgataatg tcggtgttga
    16681 gttgcatctc acctcgtctg gcttcatcga gcaggttttc catcagatgc gtggtttcct
    16741 gcaaaacgct gaagccaaaa gttcctgccc ctcctttgat cgagtgggca gcccgaaaga
    16801 tggcattcaa ttgttcggca tctggcgctt ccggctgcaa aaccagcaga tgctgctcca
    16861 tatcagccaa cagttcgtcc gcttcatcaa aaaatgtctg ataaaaatcg cttatatcca
    16921 tgctcacgct gtcacctcgg ttcggctgat ggcattgtgg gaacacagac ctgtggtgca
    16981 acctcaggtt tttccagggc gcttactggc tcattctggc tttcggcgtt ttcatgcaaa
    17041 atggcctgtt cggcttgttt gttcagtacc agcaggctga tgcgacggtt gacggcatca
    17101 tcaggtccgc gatcgcttaa gcgcatcgtt gccgccatgc cgaccacacg taacactttg
    17161 ccgctatcca accctccgac catcagttcg cggcgggatg cattggcccg atcggcagaa
    17221 agctcccagt tgctgtaacc tttctcacca ctggcgtagg ggaaatcatc ggtatgaccc
    17281 gaaaggctaa tacggttggg aataccattc agaacgggcg cgatggcacg cagaatgtcg
    17341 cgcatatagg gttcgacgtc ggcactgcca gttctaaaca tcgggcgatt ctggctatcg
    17401 atgatctgaa tacgtagacc ttcctggacc agatcgattt tgagatgtgg acgtaacgcc
    17461 cgcagtttcg gatcggactc tatcaattgg tcgagatcgc cacgcaattt ccgcaatcga
    17521 ctttgctcca tgcgtttttt caactcttcg atgttcggct gcttattcac ttccccctgg
    17581 ctttgggtgt aatcatcgcc accgccggga attgggcttt cactattaga aatgcgatcg
    17641 ccgcccgtaa ccgcagtcgc cagtggagtc cggaagtact ccgcaatctg aatcaactct
    17701 tttgggctgg agatggagat cagccacatc accagaaaaa aggccatcat cgcggtcata
    17761 aagtcggcat aagcaatctt ccacgatcca tgtgctgccc cgtggctttt ggctttgcgt
    17821 cgtttgacga caataatcgg atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt
    17881 gttgcggatt tttcaccgca cgcacatgct cttccagttc aataaacgac ggacgttcgc
    17941 tggaatagag cgttttgcga ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat
    18001 tagaaagcag tgtgactttg acgcactgca tcattttgct ggtttcggcg cttttctgac
    18061 gtaaaacact cgctaatggg gaaataaatc cgtaagccaa taaaatgccg aggaaagtcc
    18121 ccaccatcgc atgtgcgata agcgccccca gctcggcggc aggacgatcg gctgaaccta
    18181 acgcgtgaac gacccccatt acagccgcga caataccaaa cgccggaagt gagtccccga
    18241 ccagcgccag actgttcgcc gggacttctg cctcgctttc gtgcgtctca atctcttcat
    18301 ccatcagggc ttcgatttcg aaggtgttca tgtgaccgct gataatcagg cgcagataat
    18361 cgacgataaa atcaagcatg acgctatccg cgatgatgcg tgggtagctg gcgaagatct
    18421 cgctctcacg gggattttca atatcacgtt ccagcgaaaa catccccatc tgccgcgatt
    18481 tcgccatcaa ccgataaagc agagccagca gatccatata cattgctttg gtgtatttgg
    18541 agcgacgaaa cagcaacggc agcgctttca gcgtgccttt aatcgctttt ccattattgc
    18601 cgacaataaa cgacccaatc cctgcaccgg caataatcac cagttcagcg ggttgataga
    18661 gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg agaacaacca
    18721 ggtaacctaa taagataagc acgacatcat ccttccactg ttgaccatga caggatgttc
    18781 agtcgtcagg cgttaacgcg cgattggggc aaaaaaaagc agcggtacgt cgttaccgct
    18841 gctggaatgt tgcgccacac cgtatcagtt aaacagcctg tactctctgt tcatccagca
    18901 gttgtgggat aatatcggca ggattctggg aaagtttacg tctttttact gcccgggatg
    18961 gcggttgaca taagctgcag gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat
    19021 tgccgccgca gcagttgcag ctggaaagtt gcagtaatcc actttcaaca aaccgcacca
    19081 atgtccaggc acgggttaat gccagcagcg gtccttcttc tgcttgcggg cactgttcaa
    19141 ggtataaacg gtaggctttg atcaccgcat cgacgccatt acacaaaccg gttttcagta
    19201 aaaactgcca tgcattacag aacatcgaag catgaacgtt ttgttcccaa gtcataaacc
    19261 agtcggttga gaatggcagc atgcctttcg gcggtgggct tccgcgcagt tctttataaa
    19321 gttttatcag gcgtccgcga cttaactgtg tttcgctttc cagcatctgc aaacgagcgc
    19381 ccagggtgat caattccatt gccagctgaa tatcccgcgc ttcctgaaca atgctttttt
    19441 cactcatgat caggcccttt tcttgcgcag cgcttcttca ggctgattaa catcattcag
    19501 caagcgtgtt gagagcatga tgccggtatg aatttgctgg agatcgtcaa cgcgggaatc
    19561 ttgcgtcaac agagtaatcg tctggtggct gtcaaaacgg aagtgacaaa ccagctgatt
    19621 ggtttctgcc agcttaacca tttgcggaag agtcagtgcc gctaatgttg tcgccatttc
    19681 ttcatttatg ccgagacgaa acatagcgga cgctttgtcc tgaacaatca gacgctgtgc
    19741 aagtagtaaa tatgacaagt tgatgtcata aatgtgtttc agcaactcgg aggtatgcat
    19801 tattcccacc cagaataacc aactttattt ttatgcggtt tcaccgcacc ccgtgatgtc
    19861 gccgggaagc cccggtaaaa aataattagc attagaatag ttgcgataag ctgcaataag
    19921 cagagccacc tttttggttt aataagtcct tacaaataga agggggtctt tacacttatc
    19981 taagattttt cctaaatcga cgcaactgta ctcgtcacta cacgcacata caacggaggg
    20041 gggctgcgat tttcaataat
//
LOCUS       DAZKFZ010000066        20046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000066 DAZKFZ010000000
VERSION     DAZKFZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(486..917)
                     /locus_tag="ACHOQW_002260"
     CDS             complement(486..917)
                     /locus_tag="ACHOQW_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFP9573336.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(985..3006)
                     /locus_tag="ACHOQW_002261"
                     /pseudo
     CDS             complement(985..3006)
                     /locus_tag="ACHOQW_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3140..3265)
                     /locus_tag="ACHOQW_002262"
     CDS             complement(3140..3265)
                     /locus_tag="ACHOQW_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP9573337.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(3207..3356)
                     /locus_tag="ACHOQW_002263"
                     /pseudo
     CDS             complement(3207..3356)
                     /locus_tag="ACHOQW_002263"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3530..4291)
                     /locus_tag="ACHOQW_002264"
     CDS             complement(3530..4291)
                     /locus_tag="ACHOQW_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP9573338.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(4288..4722)
                     /gene="psiB"
                     /locus_tag="ACHOQW_002265"
     CDS             complement(4288..4722)
                     /gene="psiB"
                     /locus_tag="ACHOQW_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP9573339.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(4777..6735)
                     /locus_tag="ACHOQW_002266"
     CDS             complement(4777..6735)
                     /locus_tag="ACHOQW_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9573340.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(6801..7034)
                     /locus_tag="ACHOQW_002267"
     CDS             complement(6801..7034)
                     /locus_tag="ACHOQW_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9573341.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(7091..7543)
                     /locus_tag="ACHOQW_002268"
     CDS             complement(7091..7543)
                     /locus_tag="ACHOQW_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9573342.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(7569..7775)
                     /locus_tag="ACHOQW_002269"
     CDS             complement(7569..7775)
                     /locus_tag="ACHOQW_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9573343.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            7845..8294
                     /locus_tag="ACHOQW_002270"
     CDS             7845..8294
                     /locus_tag="ACHOQW_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573344.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVRGGA"
     gene            complement(8380..8943)
                     /locus_tag="ACHOQW_002271"
     CDS             complement(8380..8943)
                     /locus_tag="ACHOQW_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP9573345.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(8990..10351)
                     /locus_tag="ACHOQW_002272"
     CDS             complement(8990..10351)
                     /locus_tag="ACHOQW_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP9573346.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(10403..10633)
                     /locus_tag="ACHOQW_002273"
     CDS             complement(10403..10633)
                     /locus_tag="ACHOQW_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573347.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            11150..11659
                     /locus_tag="ACHOQW_002274"
     CDS             11150..11659
                     /locus_tag="ACHOQW_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573348.1"
                     /translation="MSRDPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDRGCATGT
                     VAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKRKKKKNS
                     NRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRPPPPALY
                     HLFLLPCKM"
     gene            complement(11629..11820)
                     /locus_tag="ACHOQW_002275"
     CDS             complement(11629..11820)
                     /locus_tag="ACHOQW_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573349.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(11817..12239)
                     /locus_tag="ACHOQW_002276"
     CDS             complement(11817..12239)
                     /locus_tag="ACHOQW_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP9573350.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(12286..12711)
                     /locus_tag="ACHOQW_002277"
     CDS             complement(12286..12711)
                     /locus_tag="ACHOQW_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9573351.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(13129..13905)
                     /locus_tag="ACHOQW_002278"
     CDS             complement(13129..13905)
                     /locus_tag="ACHOQW_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573352.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(13951..14505)
                     /locus_tag="ACHOQW_002279"
     CDS             complement(13951..14505)
                     /locus_tag="ACHOQW_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP9573353.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(14399..14620)
                     /locus_tag="ACHOQW_002280"
     CDS             complement(14399..14620)
                     /locus_tag="ACHOQW_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573354.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(14621..15304)
                     /locus_tag="ACHOQW_002281"
     CDS             complement(14621..15304)
                     /locus_tag="ACHOQW_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP9573355.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(15381..15686)
                     /locus_tag="ACHOQW_002282"
     CDS             complement(15381..15686)
                     /locus_tag="ACHOQW_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573356.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(15690..16592)
                     /locus_tag="ACHOQW_002283"
     CDS             complement(15690..16592)
                     /locus_tag="ACHOQW_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9573357.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(17287..18258)
                     /locus_tag="ACHOQW_002284"
     CDS             complement(17287..18258)
                     /locus_tag="ACHOQW_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFP9573358.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(18258..19424)
                     /gene="sopA"
                     /locus_tag="ACHOQW_002285"
     CDS             complement(18258..19424)
                     /gene="sopA"
                     /locus_tag="ACHOQW_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFP9573359.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
BASE COUNT         4557 a         5910 c         5054 g         4525 t
ORIGIN      
        1 atttttgttc tcttttaaaa tatattccac gagcgtcttg tgggaccttt tgctgttata
       61 ggggggcagt ttggatatcg aaaattattg tacgttaagg ctgaatttca atcgcaaagc
      121 ataaccttac ctttctgctt acttatgcag ttcaaagagt accaactctg caattttcgt
      181 tgccacagat gcaatatcgt tactttccag tcaccatgcc gctgaaagcc cacaccatgc
      241 gataatccac atcaagagcc gctttctgtc cagacccgct gttgtgaggt gtgaggtgtg
      301 aggtgtgagg tgtgaggtgt gaggtagcct gagtttaacg gacaggccga tgaaggacgc
      361 cgttaccggg ataatgtaag aatctttttt tgagtatgtc aacataacct gaggcaaagt
      421 acctcaggtt ccagtaatca gccgacctga cagtcttctg ttagccctac agatatgtcg
      481 tgtagctaac cgcgcatgtc aaagccggat ttttgaaaat ctctataaac ttccggattg
      541 ttgaaggccg ggaatttagc tttatgggct gctgacttta tcgcttcgca ataggcttta
      601 tcgccattac tggttgatat ttttaacgcc gtgccatccc gggagaattc catatgcaac
      661 ctgcattttt tccttttcca tttttgcgga tcatcaagtt tagcattaat tgcatccctg
      721 attccccgtg cctgctcccc ccattcatcc tgatcatccc aacgtcctga actgcaacta
      781 cccagggctg tagttttatg gcaatttgaa ggatgtaatg gcgcacatcc cgccacaaag
      841 ctgactaaaa tagccagtat aacgattttc tttaccccca tatattgctc ctcaacccat
      901 atagatctca gtaccatagc tctgtcagag caaagaaaat cttaactaat acaagttaag
      961 atttttaata tccactcctc atccctacat tccaggtgat ggtggcgtcc actcattatc
     1021 ctgaactgga gcataagatg cgccatgccc cccggatatg cttttctgtt gcctgatata
     1081 ttcgtggtag tactgttcac tacgctgacg atgtaaatcc acatccgggc ggctgattac
     1141 aaagtgtcca tttccggtta ttccagctgc attatgccag gccaccattt ccgcaaatac
     1201 atcattaaac tgttgatatg agccttccgg ttcaaaaccc attcctggct cacgatcaaa
     1261 caatacagga gcaatcagat tcagcttatc caccagttca tttctgtcct gatgatggta
     1321 gaacaccaca cggtccccat aatccacaca gactaactcg ccattcctcc gatcacgaaa
     1381 acgcacactg ccatctttct gcagacgggc ttcgagaacg ccagtgtctt cataaagtgg
     1441 agttcctccc ggttcacaga gaatcacgca gcggtcggca ttcgttgttc gtcgcttgtc
     1501 tttgcgacga tcacgataat cccagccacg taactgtgat aatgcagctc tgtctccctg
     1561 taccgcctgt tgctccaccc actgccggta ggacaaagga taccacttac cctcctcaat
     1621 cagtgacaac cgctcttcct tcacactctc cttcagcctg atcagcgcct gcatacgctg
     1681 cacttccgca atatggtaat gcagcttacg caattgtgga tcccggaact gcacacggat
     1741 atacgccttg cgccgacggc aggcagcatg aatttcccgt aaccgttctc cgtaacgtaa
     1801 gtcgggttta cgccagtgct ccttccatgc aagataacgc gcgcgcaaat cttcccgcgc
     1861 agctgcacgg gcttcacggc gttcacgacg cagtacagga tcgcgccgga agccgggctc
     1921 cacttcatcg ctggcagcaa gttccggatt gtagcgacat tcaggcttca cccgttcaaa
     1981 tatatctgca cctgcaatct caaatggccc tgcctgcggc tcagccctgg ataacgtcaa
     2041 atccgggtgg atactgctgg ccttcactgg tgtgagctca tgattaaacg catccacaat
     2101 cacaagacca tgatgctgtt tctgcaacat aagcccttta cgcgcaaaca tctcatggca
     2161 cgcctgccag ctgaaattcc cgcccgcgat catatcttca atcaacgact cacactgaac
     2221 agttgtgtac ccccacagac tttccttatc agaaaacagt tcgcgcttta ttgctccctg
     2281 tggacctgtc cccatccgca tacgatacca ttcgtaatca ctgacagcct gagtaccaag
     2341 attacgtccc cggaaccgtt ctccgggtat aacctgagta aaaatatcct tcggtacagg
     2401 ctgccatcca ccatccagat cctgcatata atccagcgtc attaacggcc acacactgtc
     2461 tgcacgtact ggtgtctgtt gtttatcaaa tccatcatgc agtaccagat gcccgtgctg
     2521 gatccccatc cataatccag ccgttgccag agttctgtgc acatccaggc aactttccag
     2581 ctcaccattt ctcgccattt ccggtaagcg ttcggcaaaa tgacggcagg cgtactgttt
     2641 cagactctcc gtttctgcca ctttttccgg ccggatatca acgtttatgg cttccggatc
     2701 ataacgaccg ggtgtcccca cctgactgaa aatatctgtc ggtacaggct gatattcccc
     2761 cagtttccgc cccagttttt cagctgtcca taacggtccg aacgaactga gtgcaacacc
     2821 ttcccgcgct ctgtcccagc catccttcac caccagttcc ccttcctgca tcgtgatata
     2881 cagaccatca ctggccagcc gtttatgcaa agataaccag tcctgtgcgg gctcttcacg
     2941 cagcccggca atcgacatct gagcaatata ttccctgaac gtctgctttt tcccgcgtcg
     3001 ccaggctgtt catgcccgta caatgccagc aaggagaaag gggccaccgg cgaaccagca
     3061 gcccctttat aaaggcgctt cagtgcagtc agaccagcat cagccctgaa aaggcgggcc
     3121 tgcgcccgcc tccaggttgc tacttaccgg attcgtaagc catgaaagcc gccacctccc
     3181 tgtatccgtc tctgtaacga atctcgcaca gcgattttcg tgtcagataa gtgaatatca
     3241 acagtgtgag acacactatc aacacacacc agacaagaga gctgcgtggt agtttcatgg
     3301 ccttcttctc cttgcgcaaa gcgcggtaag aggctatcct gatgttgtct aagcatgcag
     3361 gggcctcgtg ggttaatgaa aaattaacta cggggctttt gtccttctgc cacacaacac
     3421 ggtaacaaac caccttcacg tcatgaggca gaaagcctca agcgccgggc acatcatagc
     3481 ccatatacca gcacgctgac cacattcact tttcctgaaa ataacccgct cattcaggct
     3541 gtttacgggc aatccgtgtg attgttgccg catcacgcac ctcccggagt ttgttcggca
     3601 ccatccaccg ctccatctca cggacagcat gaccggcctc ctttgctgcc agactggctt
     3661 ccgcgataac ctgccagaat ggcatatcct gccactgcca gcgttccatt ccggccagtg
     3721 acggaaaacg ctcgccccac tgccagaaca gggtttccag gtcatgctct gccacccgat
     3781 cactccagcg tgcataccag cttccgactg tttccggtga atggaacgcc agttcgattt
     3841 cagcctgggc gaccagcgcc tggtacgcac gggctttctg ctctgcctcc ctcgcctgct
     3901 ggcggctgta cttctgcatg accagcgcgg atttatggcg actgcgttcc ctgacacaaa
     3961 accgcaccga cgggaacaag cgttctgctg catcaccggg aaggggaagg aggcagacct
     4021 cgccctgtgt ttcaatcagc acatcgatgg catacaacca ctgaagacgg tttccgtgca
     4081 gttcttctgc ggtcagggac ggcatgaaaa atcgcaggtc agacagcgat atcctgcggc
     4141 ttccgttcag acagcggaaa aacgcccccg catacggata ttccgccagc gtgtttccct
     4201 gattgcggcg tttttccgtc tctgccactg cccgccatgc ggcctgttgc tctgtgctga
     4261 ggggaaccag tgcctgtgaa cgtgcgctca tacctgacct ccttccgcca tcaggatatg
     4321 gatgatgtcg ttaacgctgt atccctgcgt atccagtgac gccgccagtg ccagtgaatg
     4381 gctgacggct tccggtgcaa accgccgctg tacctgaacc accgcaaacg gctcaccacc
     4441 ggcattcacc agaaccagca cccagacctg ggagacatcg cccggtgaac ataacgccag
     4501 gtgaaaacgt tcgtgggcag gcgtgaaacg gacctggacg gggaaaaagc cgccaaactc
     4561 gctgccgtac tcgccgttca tcgtccagtt gtccggcgca tccagctccc gcatcacggc
     4621 gtgtgtcagc tcacggcgtg catcacttcc ggcagcccgg atatcttcat attcctgtgc
     4681 gttcatggta tgtaatacat tcagagtcag ttcagttttc atcacggtat ctcccttgct
     4741 gccgccccgt ctccggcggg gcggtgtggt tgttcatcag gcggcatcag ccaggttatc
     4801 ggtgcgttca gtggcatccg tctgtgggcg tggtgcacac atccagccag gcacccagcg
     4861 gttgtctttc atatggtgct ctgcatgttc agccgcatcg cctttcttca tcttctccgc
     4921 gtcccgtgcg gcaccggata gtcctgcctc attcagggct gcgataatct gcggcttttt
     4981 caggtgtccg aaaaagtttg ctttcgtcgg ctgccaccag tcgcgcatgt gaaagccgat
     5041 ggcgctctcc agcgtgtcca gcggactgcg tgacgtgtga ccatactcac gggtctggac
     5101 accgtggata ctgcacgcgg tgcagaaact gagcagtgat aacagcacct cctgactgag
     5161 tgacaggaac gtcgtcatat cccgtgccca tccatccggc agcagggcgg caagacgggc
     5221 tttttctgcc atcatcgcca tgaatgcggc accttccttc cctgacggcg catcgctggt
     5281 cagcgaataa tgctcacatt ccaggcggat tctggcggga ttgctgtacg ctccactgcc
     5341 aaatacattc agacagagtg tccatgccag aagtgccagg gatttgtctg attgctgcat
     5401 cagtgccgcc tggactgcca gcgtgcgctc tgaggacatt ttcgtcagca gcggcagact
     5461 gatttcctcc actgaggctt tctcctgcac ctgttccgtg cggtaatcgt tatcagccac
     5521 atcatcctca ctgcgcaact gcacaccacg ctggacacac acgttgccat aacgccagga
     5581 caccaccacg ccacttccgg cacgcatctc cggcgtccac gccctgacct tcgccatgca
     5641 gcgcatcagc ttcatttcgg cttccagcag gtcggattcc tcacactggt tttccagcgc
     5701 atcgtaacgt gccatcagtt cgttcaggcg ttcttcttct gcctccgtca gcaccgcttc
     5761 cggctccggc agactgcggt atgttccggc atcctcacgg cagaaaccaa caggctccat
     5821 acgtccggcg caccattccc agccttcggc ttcccgaagg tgttcggcga cagcctggag
     5881 tttttccagc agggcggcat cgagcgccac gcagtccaca tagccaccct cgtcgtcact
     5941 gaacaaatcg gtgcgcagtt catctggcga gaaggtatca gcccccacga agcggaattt
     6001 actgttccct gccaccgcca cttcactttc ggtcaccaga cgacgaatgg tctgtacttc
     6061 cggtttaccg ccccatcccg actgacaggc ggcttcaaac acctgcacct gacgcgcggt
     6121 gtcgttctcc agcgccagcg cctgacagtg ctcggtggtg atgcggtctt ctgccagcgc
     6181 atcgaggatg acaggcgcaa ggtctgccag tttcagcatt cgctgaacgt ggcggggcga
     6241 ataacccagc aaatcaccga tttgtgcagg tgttttgcct tcctgcgcca ttgcacggaa
     6301 tccggcaatc tgttcggcag ggtgcatatc ccgacgatga ccgttctcgg tcatcgatgc
     6361 ggcagtcgcc agctcctgcg gaatgacctt cacacggaca ggccagtcag ccggaatgat
     6421 gtcacgctct gccagcatgt tgagtgctgc cagtcggcga ccacctgcgg cgacaccgta
     6481 acggtcacct ggcagggcat gaacaaccag attctgtaac aggccgacac ctttaatgga
     6541 gtccgccagt tcgctgacgg actccgcaga atacggcacc gtgcgcacat tcagcggtga
     6601 cttaatcagc gaggccagcg gcacgctcac ttcctccgtc tgcgccagca gggccgacag
     6661 gactgtttcc tgcgtttttg caggtttacg gctggattta cgctccgttt ttgccttaga
     6721 ctctgtaact gacatagtta gtacctccca aaacggtaat aatgattgtc tgccggacgc
     6781 gttcccgtcc ggcacctctc ttacttaata atcccgaaat cacggatgac atggttcatc
     6841 cagtacccgg caccgtcctc aaaattccag gtacgccaga ccattgcacc gttattacgg
     6901 acaaccaggc gaaactgttc gccgtggtca ttctcgataa agacgttccg gtactgtgcg
     6961 gcaacggctt cggcatgttt gcgggtaaaa ggaccgtcag gcagtccctg aagtattctg
     7021 aaatattcgc tcatcctcat ctctccggtg atgccccgca caggacgggg cggttgtcac
     7081 agtcagtcgt tcagaacggg atatcgtctg aaaacccgta atcgtcaccc tccggcggtt
     7141 gcggctgagg ctgaccgctg aactgttgcc cctcttccgg ctgagtctga gcacctgcgg
     7201 cacgtaccag catctgcatg gtgcccgtgg tcttaacaag aatttcggtg acgtaacggg
     7261 tgataccgtt atcctcccag ctacgggtgc gaagctgacc ttcgatgtag acctgcgcgc
     7321 ccttgcgcag atattcacct gccacttccg cgagcttgcc gaacagcacc acgcgatgcc
     7381 attctgtctg ctcccgcatc tcccccgtct gtttgtcacg ccagctttct gacgtggcca
     7441 cctgcaggtt tgccactgcg cccccgttgg ggatgtaacg gacttccgga tcttttccca
     7501 ggcgtcctac gaggatgacc ttgttaatgc cacgaactgc catgatgtgt ctccttctgt
     7561 tgatgttttc agcccccatc atcttttgag tttcgggagc cgcggttttg acgttttgcg
     7621 gggacctgcc ttttgttccc tccggtattc accatcacct ttttgagaac cttcgccgca
     7681 aggggcatca ctgccacggc cggaaacgac tgtccggggg cagaccgcaa aactttttgc
     7741 ctgcttttgg gcaaaaagtt ttgcgggatg tgcatttcac ccctggactg gcggagacgg
     7801 ttgtggctgt ctttgccccc ggcgacaggg gcttaaacag gtgaatggca ccggacacaa
     7861 caacgggcag gcacccgccg caacgacgtg aagcgcggtt tgcgaaacgg cgttgcccct
     7921 tacatcccgg cggccttttg ccgggatgcc gggagcgcag gccacggcgc gacagaggcg
     7981 gcgcgaccgg aacggtcgct gcaagcccgg gtttcgcgtc agggatggaa gcccgccagg
     8041 gcggagacag ctccgctggc tccgttcacg acagcccgcc ccgccagggg gacgcccgga
     8101 aaaggcataa gcaggagaac aacaaaacag acagtaatca gagcagcaga aaaaagaaga
     8161 acagtagcag tagcagtagc agtagcagta gcagtagcat gaacaacaac atcatcagta
     8221 acagacaaat gacaggtctg aaaagacaga agcccacagc agaatgtccg cgtaatgtaa
     8281 ggggcggcgc gtgagcgcgg cccctttcag cgccaccggc gcgcggtctt ttcccggcgc
     8341 gggcagacgg gcatttatcc tcccgcctgc ccggagtcat caggcacgca gacgaataat
     8401 catcgtcgga acatcggtat aagcaaacgt gccgcgtggc agctcctcgc ggacgtcaga
     8461 aaacggtaac agcttctcct gctggcgcgg cccgttcaga cagacggcga ccagcacgcc
     8521 acccggacgc aacagggaaa aggcccgcag gatatgccgg atatcctgcc cgtggctgaa
     8581 cggcggatta atgataaccc ggctgtaata ctgcaccggc tgccattcca tgaagtcgcc
     8641 acactgaacc ctgacaccgt taaaattttc ccgcagatac cggaccagcc cgctgttgat
     8701 ttccaccgca tcacacatgg cctccggtgc ggtatcccga atcgcccgca gtatggcccc
     8761 ggtgccggca gagggttcca gaatgtgatc acggttgctg atctccgcca gcgtcaccag
     8821 gcgatcacag accggggcag gagtcacaaa aagctgagat tcagcccgga tttcagcaac
     8881 cggcgcggcg ttatccagcc ttgcgcgcat cagccggata cgttctttta actgactgtg
     8941 catgtcatta tctccgtcat cccccgggga tttcccgggg ggatatacct tatttctgtg
     9001 ggatttccgt aattttcatg tcggtgatat acacattcac cagacggaaa ttattgtcca
     9061 ttgtgcggcg gtagcggtat gccccgtggt cttctgcttc tgccacactg cgcacggcct
     9121 tacagtcccg gggcagggcg gcccactgtg cctttgtcat ttcccggaaa ccttcccccg
     9181 gatagttgac taccggcgga cgcttcgcgg cctggctggc gacggcagcc tcttctgccg
     9241 atggtgcttt gtagtccgtg atgcgatcgg gcgtcacttt catcgtgccg ctgtacccga
     9301 gaaaactgta attcggcgtt gtgactgaac tcaccgcccc gttgcttttg ttcacgcgga
     9361 tgatggtcag ccactcgccc cggctgaaaa cctgtccgcc cggctcaaaa tcctgtgtcc
     9421 gggtaaccac gccgccgctt tcgtccagca tggcacgctc atagttcagg cggttctgat
     9481 agtggttaac ccagcgttgc tgatgctgaa tctgccgttc atgacagcgg atcgcaattt
     9541 cgcgggcctg ctccgtggtg atgatgtcat catccagggc cgaccataac gaccggctcc
     9601 cttcatactg gcttttttct gccgggcgcg gatacgtatc cagcgggaaa caggcgctga
     9661 tatggtcatg actgctgatg agttttgcca tattcagatc cagcgattca gcccgccaca
     9721 tcgtcagata tttctgcgac tgcgcgatgg tcttttcagc cttgcgcaaa tcagcttcga
     9781 tttttttgat acgacgccag cgaacgtccg gacgttcttt atacttcgcg tgaagcagtg
     9841 ctgaccgccc ccgttcttcc cagtattccg cacgttcaaa gagcatcacg gcacgtttca
     9901 tgccgttttc aatacgctgc gcatcacgac gggcgcggcg ttcgctgtga tgccccacaa
     9961 gaatgggctg accgggcggg atcatcgcgg ccagtctttc cacttcatca agtgcctgtg
    10021 cggattcact ggcccgcttt ccgctgtatc cggtaaaccg ctccgcccgt gcttcctgac
    10081 gttcagcgag cgtgctgtct tcatcctcaa tctctccggc aagtgagagg agcacgtctt
    10141 cccggcccgg tgtccaggcc ggcgcgacaa acagcgcctg ttttggtgcc cagcgaaaac
    10201 cggcatcatg cactttttta tacgtctccg ggtcaagacg tgacacggca tacaggcgca
    10261 gtttgttgtc atccggtgag taggtcgcgc ggtacatctg cacagggctg gcggcttccg
    10321 gggcggtcat ggtgattgct gctgatgtca taaaatgttc tccttccgtc tgttgccgcc
    10381 cctcgcgggg cggctcacct catcagttaa aagcgcgttt cagttcgagt gtgcggagca
    10441 cctgcccgaa agccgtgttt ttcatggcgg ggatgtgtac accacgcccc cggtgaaaac
    10501 tccgggctat gtgtcgcccg aaggctttca gcacggggtc aatttccgga gcggggatgt
    10561 cttcgcgtct gaaacgggga tgacggcggg gctttacagc cgctgcggaa ggtgaaacaa
    10621 tgcttacagt cataaatgga ttccttatga caaattggtt aacaatgccg tcaaaattgg
    10681 tcgcacaatt cttacggcgt ggattaagca ggtccctgac ctgctttttt catatctgca
    10741 tctgacgctt tccggtgttt tcctccttct gttcctgcgt tctcattacc tgtcagccgg
    10801 atgactgaca gaacacataa atgcgtattg ccggagcctg aaataaatcc tgatttttaa
    10861 agaacatcac ccgttgagtt tcgggagccg cggttttgac gtttcgcggg aacctgctgc
    10921 tgtattccct gtggtatgaa cttcacccgt ttgagaacct tcgccgcaag gggcatcact
    10981 gccacggcct gacacacctg tcctgggcag aacgcaaaat tttttaccgg gttctgggta
    11041 aaaaattttg tgggctgttc atttaccctg gacagtcagt gcggcaggtt gtggctgtat
    11101 cagctcccgg cgacagggac tcatacgggt gtgtggcacc cggcaggcaa tgagcaggga
    11161 cccgccgcaa cgacgtgaag cgcggtttgc gaaacggcgt tgccccttac atcccggcgg
    11221 ccttttgccg ggatgccggg agcgcaggcc acggcgcgac agaggctgcg cgaccggaac
    11281 ggtcgctgca agccagggtt tcgcgtcagg gatggaagcc cgccagggcg gagacagctt
    11341 cgctggctcc gttcacgaca gcccgccccg ccagggggac gcccggaaaa gccataagca
    11401 ggagaggaac ggaaagaaga ggaggaagag gaagaggaaa aagaagaaga acagtaacag
    11461 gcacggtaac cacagaaaaa taacatcaga aaaaaacatt ccggacacat cacaaacagg
    11521 aagggtaacg gacgacgcgt cagcgtggcc cgcttccctt ccggcaggcg tcagcctgcc
    11581 aggggttttc gcgcgggctg acaggcggcc tccaccgcca gccctgtatc acctgtttct
    11641 gctgccctgt aagatgtagt gaccgcccag gaaaacagcc tgctgattcg ccacaaaatc
    11701 gcagatttca tcaacaatct gcgcggtggt gagcggtttc tgccccattt cacgacgtcg
    11761 cgcattaatc acggctttgt aattgcgcag aatttcgcgg gtttctgtgc tgatattcat
    11821 gacatgtgct ccccgtccag cgcctgtaac acaacgtcca ttgcttcgcg ttcctccctg
    11881 acgaacgcat caccgtcgcc cccgttctca ctcccgacgg catacaggcg cttcatttca
    11941 ctgcctgcca gcgccatcac tttttccagc atcaccgccg gaactgtcac ctgacggctg
    12001 gcacgctttt cttccatcaa ctcacgaaca acgtcgtgaa caatgctgac atccgcacca
    12061 tattcaaagg catcgtccag ccgctgcatg acggtttcca gctcatcgtc tgacaggttc
    12121 cagtcctggg ctatgtggcg cacatcccca cgggtcagca ccacggcaaa aacgcattca
    12181 ctgtccggca cgtctgtatc cagtacatcg cggatgattt ctttaacagt acagtacatg
    12241 ttctcgtctc ctttacaggg gcggcattgc cgccccgtcc gttaatcagt ccagtgccgc
    12301 gtaaatcgcg ttgcattcag ggtgaaattc gatgtgacgc cacaactgcg catcgcgcat
    12361 ccggtaaagc tgggtcagtc ctgcatcacc gctgtcgtgg tacagccaca actggcggtt
    12421 aatcacaagg gaagtcagaa tgatgccgca ggcatcagca ctcacggtac ggtcaaacca
    12481 gtttgcaccg ttcaccatgt ggaaacggtc gccatccggc atcatgtaac cgccaccgtc
    12541 cggcaggcgg atgaagtgcc agaagcctcc ctgatattcc ggcatcattt tttgcgccag
    12601 cgcgtacaca tacgcctcgg catacagaaa atcgttgcca aacaggtaag gcagaaagga
    12661 gagacggtcg cactcttcaa cattcagagt gacaggtttc gcatattgca tgatgttttc
    12721 ctccgttgtt cacatattcc gcgacaccgg gcggtgccgt gcctccagaa aagccgggcg
    12781 ataaggcggg gatgcacagg gcgcaaggga agccgcagcc tgacgcacac ggctgacggg
    12841 agttgcaggg tgaggccgcg aaacctgagc gggcgaacga ccggaaaatc acggcagacg
    12901 ggaaggaggg accgggtgac cgcagcggcg aagcgtaccc ttgcatcaac gccagcccgg
    12961 ctaagatgga ggcacggcac cgcccgcaca cgacgctgag gccacataac tgaaaaacca
    13021 caaggccatg ccctgcatgg ccgggtaaag cggagccagc ggcgacttcc tttgtgctct
    13081 gtccggggac cggcaggaaa aaggcgggga tttaccccgc caacgtcatc atttccggat
    13141 ttttgccagg gcatccgcca tgctgcggat ttcgttccgg gcttcccgga ccacctcccg
    13201 cagaacacca cggtcaaacc accggcgggg cggttcatcc ggtatacgca gcacatcgca
    13261 ggccagccca ctggacagga tatgttcaat acaatcgctg accagataca tttccaccgc
    13321 cgaaaaacgt gcgccccggt tcaggatggc atcgagcacc acgggcaggt tttcgtcgtc
    13381 ctcatcgagc cagcaggggt gatcgctttc cttcacgacc aggcgaaagc ggcgcgtctg
    13441 gcgggtcagt aacgtctgac cggtggtggt cagcgccgcc cggatacgcc gcgtatcttc
    13501 tgccacccgg caactgacac tgcgtaccgc atcgcgcacc gccgcaagta cagccctgat
    13561 ttcgtcttcg gtcagccatt caccggcata cggaaaagac acctgcgttc cgtctgccgg
    13621 aagcaccggc agcgactgcc actgactgcc ggacagaaca agttccgctc cggttccggt
    13681 cagtttctga atggcggagg ccaggtgcgg cggcagtacg ccgccgtggc ggttaacgag
    13741 taacgtcgcc agccggggcg catccagcag aacataaatg ctgatccagc ccggatactt
    13801 cgggttacgc tggtcgacat ccgtctcttc cggccagccc tgcgccagca gcaggttacg
    13861 ggcgtgacga caaatcagtg cgttcagcgt tgtgttttcg ttcatgatta atcgttctcc
    13921 ctgtatcgcc gcccgcaggc ggcgataaca tcagccgttt tcgtgtgtgt tgtttttcag
    13981 caggatgcgg gcctgattaa ccaccgcctg acggcgggta acgcattccg ggacggcgag
    14041 accgtaatca cgggcggccc attcacgttt ccacagtgcc tgttcggcgg tctgaatcag
    14101 ggacgccagc agttcagccg ggacggtcac ctggcggttt tcgctcacgt tgctgataat
    14161 ctccatcgcc gcggcagaag aaataccgga ctcaattcgc tggtcttccg ggatgtcctc
    14221 caggcgcgcc aggacggtcc tgatttcagg atcggacagg gaaatatcca ttccgtcagc
    14281 gagggcctga atctcttccg gggaccagat aaccagcatc agcggtgtat atcccggata
    14341 ctttgctgcc agctcccggc atagcgtttc gcatgttccg tacattctcg tctccttctc
    14401 aatctttaaa gcacgcatcc agttccagaa tttcggtata cgcctccatg aacaggcgat
    14461 tggcgcgata cagcgtctgg cgtgccaggc gttcaacaag cacatcggaa tcaaagcgac
    14521 agcgatgctc catatcgttc agcctggccc gaaggtcaca catggtgttg gccacctccg
    14581 ccacgtcggc gcgaagtttt tcatgtccgg catagttcat ttacgcagcc tccggcataa
    14641 accagtcatc atttgcggcc ccctgctgca tggcccgctg tacggcagcc agacgttgct
    14701 gtccggcacg gtgatactgt tcaagcagct cgataccgat ataccggcgt ccggactgaa
    14761 gggcggccac acaggttgag ccgctgcctg caaacgggtc cagcacaatc gcgttcgggt
    14821 gtgtgaagct ctcaatcagt ggttgcaggc tggtaacagg cttttccgtc gggtgatgac
    14881 gattgcctga atatttccag cccagcacgt cgggcaatgg cttctgaggc agcgccggac
    14941 gcccttttgc caggatgtaa gcgcattcgt ggcgatagcc cacatatgcg gccttcgaag
    15001 tgtaggtttt ggtaaacacc aggtggccaa ccacgctgaa tcccgcattt ttccaggcgg
    15061 ccataaagcg atcgacgcgg ttccagccat agaagctcac catcagcgca tcttttttca
    15121 gcacgcggta catttcatta caggccggtt gcagccattc atcggtttta tcgccggcga
    15181 tggtacgccc ctgacggtca cggaaaccga cgagatatgg cgggtcggtg aggatgaaat
    15241 cgacaccatt gcccggaatc gtggccataa cacgcacgca gtcaccctgg ataaaacggg
    15301 acataacagc actccttctc ttacagaggg caccgggagg gcgcccctgt ggcgcccgtt
    15361 ccccgcggcc tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac cgtggaatat
    15421 cgcagccgtc gtctttttga cggcgagaat atgccgcgaa agcggcttga agggcgttca
    15481 tcagaaaagg gcgacgggct ggcgtacact gtcaccgatg ccagccggga gcggtaaaag
    15541 cgcccgtccc gtgcccggcc ggacgcggga cactgaccac aggcagcgtt cctgccgcgc
    15601 cgcgtcagcc ggcgcagtaa aaagaggagc ccggcgacat cctttaccgg aaaagaaacg
    15661 ggcggttgcc cgcccgtctc cgtcatactt catccaccgt aatgcgccac cttgtcgact
    15721 atccactccg gcgcggtgac ttccggcagc tcatcatcat ctgtcgggga agaccattcc
    15781 agttcccccc acagcacatc aacaagctct ccacgctcat atcgctgcca gccacagaaa
    15841 ttgcagccct gcccggcgta ccagtgctcc agcatgcaac tgaaacggcg gcttaatact
    15901 gcaataacgt cgctttccgg ctgacaccac ggcgtgtcga aatcaacctg aatgaagttg
    15961 tctccctgac tgctgatatt cacctcatac ccacaaggcc acttcacgcc atactgttcc
    16021 gtataccagt gatatgcaga aggaacaccg ttcagaacac caccgttaaa gccattcact
    16081 tcaacatcca ggcgagtgga cagaaccgtc agcaggtcaa acggcatggt tttttccggc
    16141 aggacgttgt cacacagacg gttccaccac acagatacat cctcgttact ccagatgtcg
    16201 caccagtcac ctctttttgg ggtgaaaaga gcggtaatgc tttccctggc atcatccggt
    16261 aattcttccc agcgacgccg gccagtaccg ctctgcagcc agagttcatg cagcatcagg
    16321 cagttttgct catccagaag tacaccgtcc tgcaggtgtg tcagccagcg ggtgaacgcg
    16381 atattctccg gcgatacaac gccacgcccg gcagccgtca gtccggggca tggttcaaac
    16441 cgcacatctt cagtggtctg cagaagtccg gcacttccgg ccagaaacag ctgaatacct
    16501 tcatttgtgg cgcggcgata aagcggtgtg actgcaccgc tggccagtcg tttaatctca
    16561 gcgatctgtg ccggttcacc agaaaaatac atacgattcg agcaccagtt tggcatggga
    16621 attctcctct cacatgcggc ctccatcagg ccgtgccgca cctgtaaccg ggcgaccgca
    16681 tacgaatgca ggtgcggccc cgaagccgca gcgcgtggag gaggaacgac gaaacgtgac
    16741 cgggtgagga ggccggcgac agcgacacac ttgcatcgga tgcagcccgg ttaacgtgcc
    16801 ggcacggcct gggtaaccag gtattttgtc cacataaccg tgcgcaaaat gttgtggata
    16861 agcaggacac agcagcaatc cacagcaggc atacaaccgc acaccgaggt tactccgttc
    16921 tacaggttac gacgacatgt caatacttgc ccttgacagg cattgataga atcgtagtct
    16981 cacgctgata gtctgatcga caatacaagt gggaccgtgg tcccagaccg ataatcagac
    17041 cgacaacacg agtgggaccg tggtcccaga ccgataatca gaccgacaac acgagtggga
    17101 ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga
    17161 ttatcagacc gacgatacaa gtggaacagt gggcccagag agaatattca ggccagttat
    17221 gctttctggc ctgtaacaaa ggacattaag taaagacaga taaacgtaga ctaaaacgtg
    17281 gtcgcatcag ggtgctggct tttcaagttc cttaagaatg gcctcaattt tctctataca
    17341 ctcagttgga acacgagacc tgtccaggtt aagcaccatt ttatcgccct tatacaatac
    17401 tgtcgctcca ggagcaaact gatgtcgtga gcttaaacta gttcttgatg cagatgacgt
    17461 tttaagcaca gaagttaaaa gagtgataac ttcttcagct tcaaatatca ccccagcttt
    17521 tttctgctca tgaaggttag atgcctgctg cttaagtaat tcctctttat ctgtaaaggc
    17581 tttttgaagt gcatcacctg accgggcaga tagttcaccg gggtgagaaa aaagagcaac
    17641 aactgattta ggcaatttgg ctgtgttgat acagcgggta ataatcttac gtgaaatatt
    17701 ttccgcatca gccagcgcag aaatatttcc agcaaattca ttctgcaatc ggcttgcata
    17761 acgctgacca cgttcataag cacttgttgg gcgataatcg ttacccaatc tggataatgc
    17821 agccatctgc tcatcatcca gctcgccaac cagaacacga taatcacttt cggtaagtgc
    17881 agcagcttta cgacggcgac tcccatcggc aatttctatg acaccagata ctcttcgacc
    17941 gaacgccggt gtctgttgac cagtcagtag aaaagaaggg atgagatcat ccagtgcgtc
    18001 ctcagtaagc agctcctggt cacgttcatt acctgaccat acccgagagg tcttctcaac
    18061 actatcaccc cggagcactt caagagtaaa cttcacatcc cgaccacata caggcaaagt
    18121 aatggcatta ccgcgagcca ttactcctac gcgcgcaatt aacgaatcca ccatcggggc
    18181 agctggtgtc gataacgaag tatcttcaac cggttgagta ttgagcgtat gttttggaat
    18241 aacaggcgca cgcttcatta tctaatctcc cagcgtggtt taatcagacg atcgaaaatt
    18301 tcattgcaga caggttccca aatagaaaga gcatttctcc aggcaccagt tgaagagcgt
    18361 tgatcaatgg cctgttcaaa aacagttctc atccggatct gacctttacc aacttcatcc
    18421 gtttcacgta caacattttt tagaaccatg cttccccagg catcccgaat ttgctcctcc
    18481 atccacgggg actgagagcc attactattg ctgtatttgg taagcaaaat acgtacatca
    18541 ggctcgaacc ctttaagatc aacgttcttg agcagatcac gaagcatatc gaaaaactgc
    18601 agtgcggagg tgtagtcaaa caactcagca ggagtgggaa caatcagcac atcagcagca
    18661 catacgacat taatcgtgcc gatacccagg ttaggcgcgc tgtcaataac tatgacatca
    18721 tagtcatgag caacagtttc aatggccagt cggagcatca ggtgtggatc ggtgggcagt
    18781 ttaccttcat caaatttgcc cattaactca gtttcaatac ggtgcagagc cagacaggaa
    18841 ggaataatgt caagccccgg ccagcaagtg ggctttattg cataagtgac atcgtccttt
    18901 tccccaagat agaaaggcag gagagtgtct tctgcatgaa tatgaagatc tggtacccat
    18961 ccgtgataca ttgaggctgt tccctggggg tcgttacctt ccacgagcaa aacacgtagc
    19021 cccttcagag ccagatcctg agcaagatga acagaaactg aggttttgta aacgccacct
    19081 ttatgggcag caaccccgat caccggtgga aatacgtctt cagcacgtcg caatcgcgta
    19141 ccaaacacat cacgcatatg attaatttgt tcaattgtat aaccaacacg ttgctcaacc
    19201 cgtcctcgaa tttccatatc cgggtgcggt agtcgccctg ctttctcggc atctctgata
    19261 gcctgagaag aaaccccaac taaatccgct gcttcaccta ttctccagcg ccgggttatt
    19321 ttcctcgctt ccgggctgtc atcattaaac tgtgcaatgg cgatagcctt cgtcatttca
    19381 tgaccagcgt ttatgcactg gttaagtgtt tccatgagtt tcattctgaa catcctttaa
    19441 tcattgcttt gcattttttt attaaatctt gcaatttact gcaaagcaac aacaaaatcg
    19501 caaagtcatc aaaaaaccgc aaagttgttt aaaataagag caacactaca aaaggagata
    19561 agaagagcac atacctcagt cacttattat cactagcgct cgccgcagcc gtgtaaccga
    19621 gcatagcgag cgaactggcg aggaagcaaa gaagaactgt tctgtcagat agctcttacg
    19681 ctcagcgcaa gaagaaatat ccaccgtggg aaaaactcca ggtagaggta cacacgcgga
    19741 tagccaattc agagtaataa actgtgataa tcaaccctca tcaatgatga cgaactaacc
    19801 cccgatatca ggtcacatga cgaagggaaa gagaaggaaa tcaactgtga caaactgccc
    19861 tcaaatttgg cttccttaaa aattacagtt caaaaagtat gagaaaatcc atgcaggctg
    19921 aaggaaacag caaaactgtg acaaattacc ctcagtaggt cagaacaaat gtgacgaacc
    19981 accctcaaat ctgtgacaga taaccctcag actatcctgt cgtcatggaa gtgaaccgcc
    20041 ccgggt
//
LOCUS       DAZKFZ010000067        19761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000067 DAZKFZ010000000
VERSION     DAZKFZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            197..814
                     /locus_tag="ACHOQW_002286"
     CDS             197..814
                     /locus_tag="ACHOQW_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP9573360.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(811..2493)
                     /gene="ligB"
                     /locus_tag="ACHOQW_002287"
     CDS             complement(811..2493)
                     /gene="ligB"
                     /locus_tag="ACHOQW_002287"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP9573361.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            2751..3374
                     /gene="gmk"
                     /locus_tag="ACHOQW_002288"
     CDS             2751..3374
                     /gene="gmk"
                     /locus_tag="ACHOQW_002288"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9573362.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            3429..3704
                     /gene="rpoZ"
                     /locus_tag="ACHOQW_002289"
     CDS             3429..3704
                     /gene="rpoZ"
                     /locus_tag="ACHOQW_002289"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9573363.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            3723..5831
                     /gene="spoT"
                     /locus_tag="ACHOQW_002290"
     CDS             3723..5831
                     /gene="spoT"
                     /locus_tag="ACHOQW_002290"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9573364.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            5838..6527
                     /gene="trmH"
                     /locus_tag="ACHOQW_002291"
     CDS             5838..6527
                     /gene="trmH"
                     /locus_tag="ACHOQW_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP9573365.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            6533..8614
                     /gene="recG"
                     /locus_tag="ACHOQW_002292"
     CDS             6533..8614
                     /gene="recG"
                     /locus_tag="ACHOQW_002292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9573366.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(8599..9465)
                     /locus_tag="ACHOQW_002293"
     CDS             complement(8599..9465)
                     /locus_tag="ACHOQW_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573367.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(9468..10673)
                     /gene="gltS"
                     /locus_tag="ACHOQW_002294"
     CDS             complement(9468..10673)
                     /gene="gltS"
                     /locus_tag="ACHOQW_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP9573368.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10953..12344
                     /gene="xanP"
                     /locus_tag="ACHOQW_002295"
     CDS             10953..12344
                     /gene="xanP"
                     /locus_tag="ACHOQW_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP9573369.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            12465..14174
                     /locus_tag="ACHOQW_002296"
     CDS             12465..14174
                     /locus_tag="ACHOQW_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9573370.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(14227..16545)
                     /gene="yicI"
                     /locus_tag="ACHOQW_002297"
     CDS             complement(14227..16545)
                     /gene="yicI"
                     /locus_tag="ACHOQW_002297"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP9573371.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(16555..17937)
                     /locus_tag="ACHOQW_002298"
     CDS             complement(16555..17937)
                     /locus_tag="ACHOQW_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP9573372.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            18230..18324
                     /locus_tag="ACHOQW_002299"
     tRNA            18230..18324
                     /locus_tag="ACHOQW_002299"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18264..18266,aa:Sec,seq:tca)
     gene            18520..19701
                     /locus_tag="ACHOQW_002300"
     CDS             18520..19701
                     /locus_tag="ACHOQW_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9573373.1"
                     /translation="MALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEA
                     VAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKLPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLTD
                     LAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
                     PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEL
                     A"
BASE COUNT         4893 a         5346 c         4984 g         4538 t
ORIGIN      
        1 tttccacccc tcaattttaa cctatttttt cttatgcatt ttctcagaca agaagtcaga
       61 agaatgcatc tacgctacag aaaatagcga tttcacataa ctacaagtta tcaatttcct
      121 ctcaccttaa aaaatctcaa tcgtgacaat acgcacaaat cgctaccctg ccagacagat
      181 ttttagggag agaaccatgc tgttacacat tttgtatttg gttggcatta ctgccgaagc
      241 catgacgggg gctctggcgg ccgggcgacg ccgcatggat acatttggcg taattattat
      301 tgcgaccgcc accgcaattg gcggagggtc agtgcgcgat attctgctgg gccactatcc
      361 gctcggctgg gtcaaacacc cggaatatgt gattatcgtc gctaccgccg cagtactcac
      421 cactatcgtt gctcctgtaa tgccttacct gcgcaaagtg tttctggtgc tcgatgcgct
      481 tggactggtg gtcttttcta tcatcggcgc acaggttgca ctggatatgg ggcacggccc
      541 aattattgcc gttgtcgcgg cggtaaccac cggcgtgttt ggcggcgttt tgcgcgatat
      601 gttctgtaaa cgtatcccac tggtattcca gaaagagttg tatgccggtg tctcatttgc
      661 ctccgccgtg ctgtacatcg cgctgcaaca ctatgtttct aaccatgatg tggtaattat
      721 ctccaccctg gtattcggct tttttgcccg cttactggcg ctacgcctta agctgggatt
      781 accggtcttt tactacagcc acgaaggaca ctaaggttca aaacctgtga tctgctgggc
      841 agccagccaa ctgcccagct tcttgatttg cgcattttcc ttccattcaa taacctgtct
      901 ggcgcgcccc gatccagtcc ccggcagctg ctgccagaac tgctccgtgc taaataaaag
      961 ttgcgaccag gaccgttcat cactggcatt aagcgccgcc cgggttagcg gtattcccat
     1021 tgccatcacc cagcgagtaa aaggctgctt acgagccaga ttaaaccgat gccatagctg
     1081 cgcactttta cttttcgcga tccccggcgt gttctgtaat tgctctggcg ttaataaaag
     1141 ccaggaaaag atatgctcaa agcgatgagt ctgatgcagc gcgcgccagc cggcctcacc
     1201 aatgccatcc agccccagaa cctgttttga ccccagccag actaagcgtg aaatgaactg
     1261 ttcctgacaa acatcagaag caaagtagca ggtcaacgag ttaaagcggt tttctggtgg
     1321 tgtcggtttt gtacgttctg caccgcgcca gaccacatca tcaatgcgag gaataccctg
     1381 accggcaagg ctgacgagga tctgatcacc aggcgcaata tcccactcct gccagcgcct
     1441 gacggaacca atattcaccc gctggacttt tttatcatcc agcatgacag gtgcgagtga
     1501 cgcaaccacc gatattttac cgctcttacc caccgcaaac tgaatcgcct tcactttggc
     1561 aacctgagct acaggttgat atttccaggc caccagccat tctgcctggc ctggtagcca
     1621 atggcgggat tcgggttctt tcgcccctcg cacaaccacg ccatcggtga cgaagggtaa
     1681 tttcgctttc caccactcat tgcgtacgcg cgcaacctca tcagcatttt taaccgcacg
     1741 ggtatacctc tgcgttagag taaaacctgc ggtagccagc tcctttaaac gatcggtcat
     1801 taactgcggt ccatccggcc atgcccagac aaaaacgccc agagaattca gcgtgtcgct
     1861 atcgtcctgg cgcatcatca ggccagcaac ttttgcgcgg gcatttattc cccccatttg
     1921 ttgttggata tgcccctcgc gctggagaaa tatttccccc tgaagcgtac tgttggctaa
     1981 aggcccgcta acggtttgcg gcacagcgga aattaagcta actttctgcg tccagtcctc
     2041 gcctttcagg ccattaccgc gactgattgc tttgttcagt ttcccgtccc gataaaccag
     2101 ggttaccgca acgccatcaa cttttggctg cacccaaaga tcgctacgtt ctcgcatcca
     2161 cagacttaat gcgtttttat ccgccatttt gcgtacaccc gtatgggcaa ccggatgcat
     2221 aaccgcacca tttaaaggcg gcatcatcac gtcgcggggc tcgctcacaa agcagcgttg
     2281 ccactgcgta agacgggcgc ttaactgatc gtaaacaccg tcttccacct cactttttcc
     2341 ttccttccag tagtcatcgt cccactgttt tatttgctgt tgcaggcgga aaatttcttc
     2401 ctgtgctctg gctggcgacc aggccggaca gaccgcccac acagatgatt gccagcacaa
     2461 gatacttatt aatatcgcca tccatacttt catcttcacc tccgctgtag tcaggctggc
     2521 agatatacaa cgtggtgaag gcaaagccga gtggcaaaaa cggagtctgc gaggacgctt
     2581 cctgagaatc gtctttattg cagtaaatca caggcaaatg cggaaacagc tacgcaaaac
     2641 gcaacaactt tgcgcaaaaa gtgtgagcaa gggctacgtc acatggccgc gccgtgtata
     2701 ataagctcgt atgtaggctt tatttcgcta atcacatacg aaagatactc atggctcaag
     2761 gcacgcttta tattgtttct gcccccagtg gcgcgggtaa atccagcctg attcaggctt
     2821 tattaaaaac ccaaccgttg tatgacaccc aggtttctgt ttcacacacc acacgccaac
     2881 cgcgtcctgg tgaagtccac ggtgaacatt atttctttgt taatcatgat gaatttaaag
     2941 aaatgattag cagagatgcg ttcctcgaac acgcagaagt ttttggtaat tactatggca
     3001 cttcgcgtga ggccattgag caagtactgg cgaccggtgt cgatgttttt ctcgatatcg
     3061 actggcaggg cgcgcagcaa attcgccaga agatgccgca cgcgcggagt atctttattt
     3121 taccgccgtc taaaattgaa ctggaccgcc gtctacgcgg tcgcgatcag gacagcgaag
     3181 aggtcattgc aaagcgtatg gcgcaagctg ttgcagaaat gagccattac gccgaatatg
     3241 attacctgat tgtgaatgat gacttcgata ccgcgttgac cgatttgaag accattattc
     3301 gcgccgaacg tctgcgcatg agccgccaaa agcagcgtca tgacgcttta atcagcaaat
     3361 tgttggcaga ctgaacctga tttcagtatc atgcccagtc atttcttcac ctgtggagct
     3421 ttttaagtat ggcacgcgta actgttcagg acgctgtaga gaaaattggt aaccgttttg
     3481 acctggtact ggtcgccgcg cgtcgcgctc gtcagatgca ggtaggcgga aaggatccgc
     3541 tggtaccgga agaaaacgat aaaaccactg taatcgcgct gcgcgaaatc gaagaaggtc
     3601 tgatcaacaa ccagatcctc gacgttcgcg aacgccagga acagcaagag caggaagccg
     3661 ctgaattaca agccgttacc gctattgctg aaggtcgtcg ttaatcacaa agcgggtcgc
     3721 ccttgtatct gtttgaaagc ctgaatcaac tgattcaaac ctacctgccg gaagaccaaa
     3781 tcaagcgtct gcggcaggcg tatctcgttg cacgtgatgc tcacgagggg caaacacgtt
     3841 caagcggtga accctatatc acgcacccgg tagcggttgc ctgcattctg gccgagatga
     3901 aactcgacta tgaaacgctg atggcggcgc tgctgcatga cgtgattgaa gatactcccg
     3961 ccacctacca ggatatggaa cagctttttg gtaaaagcgt cgccgagctg gtagaggggg
     4021 tgtcgaaact tgataaactc aagttccgcg ataagaaaga ggcgcaggcc gaaaactttc
     4081 gcaagatgat tatggcgatg gtgcaggata tccgcgtcat cctcatcaaa cttgccgacc
     4141 gtacccacaa catgcgcacg ctgggctcac ttcgcccgga caaacgtcgc cgcatcgccc
     4201 gtgaaactct cgaaatttac agcccgctgg cgcaccgttt aggtatccac cacattaaaa
     4261 ccgaactcga agagctgggt tttgaggcgc tgtatcccaa ccgttatcgc gtaatcaaag
     4321 aagtggtgaa agccgcgcgc ggcaaccgta aagagatgat ccagaagatt ctttctgaaa
     4381 tcgaagggcg tttgcaggaa gcgggaatac cgtgccgcgt cagtggtcgc gaaaagcatc
     4441 tttattcgat ttactgtaaa atggtgctca aagagcagcg ttttcactca atcatggaca
     4501 tctacgcttt ccgcgtgatc gtcaatgatt ctgacacctg ttatcgcgtg ctgggccaga
     4561 tgcacagcct gtacaagccg cgtccgggcc gcgtgaaaga ctatatcgcc attccaaaag
     4621 cgaacggcta tcagtcgttg cacacctcga tgattggccc gcacggcgtg ccggttgagg
     4681 tccagatccg taccgaagat atggatcaga tggcggagat gggtgttgcc gcgcactggg
     4741 cttataaaga gcacggcgaa accagtacta ccgcacaaat ccgcgcccag cgctggatgc
     4801 aaagcctgct ggagctgcaa cagagcgccg gtagttcgtt tgaatttatc gagagcgtta
     4861 aatccgatct cttcccggat gagatttacg ttttcacacc ggaagggcgc attgtcgagc
     4921 tgcctgccgg tgcaacgccc gtcgacttcg cttatgcagt gcataccgat atcggccatg
     4981 cctgcgtggg cgcacgcgtc gaccgccagc cttacccgct gtcgcagccg cttactagcg
     5041 gtcagaccgt tgaaatcatt accgctccgg gcgctcgccc gaatgccgct tggctgaact
     5101 ttgtcgttag ctcgaaagcg cgcgccaaaa ttcgtcagtt gctgaaaaac ctcaagcgtg
     5161 atgattctgt aagcctgggc cgtcgtctgc tcaaccatgc tttgggtggt agccgtaagc
     5221 tcaatgaaat cccgcaggaa aatattcagc gcgagctgga tcgcatgaag ctggcaacgc
     5281 ttgacgatct gctggcagaa atcggccttg gtaacgcaat gagcgtggtg gtcgcgaaaa
     5341 atctacaaca tggggacgcc tccattccac cggcgactca aagccacggg catctgccga
     5401 tcaaaggcgc tgatggcgtg ctgatcacct ttgcgaaatg ctgccgcccc attcctggcg
     5461 acccgattat cgcccacgtc agccccggta aaggtctggt gatccaccat gaatcctgcc
     5521 gtaacatccg tggctaccag aaagagccag agaagtttat ggctgtggaa tgggataaag
     5581 agacggcgca ggagttcatc accgaaatca aggtggagat gttcaatcat cagggtgcgc
     5641 tggcaaacct gacggcggca attaacacca cgacctcgaa tattcaaagt ttgaatacgg
     5701 aagagaaaga tggtcgcgtc tacagcgcct ttattcgtct gaccgctcgt gaccgtgtgc
     5761 atctggcgaa tatcatgcgc aaaatccgcg tgatgccaga cgtgattaaa gtcacccgaa
     5821 accgaaatta atgttttatg aacccaacac gttatgcacg catctgcgaa atgctcgcca
     5881 ggcggcagcc tgatctgacc gtctgcatgg agcaggtcca caaacctcat aacgtttctg
     5941 cgattattcg taccgcagat gccgttggcg tacatgaagt tcacgccgtc tggcctggta
     6001 gccgcatgcg caccatggct tcggcagcgg cgggtagtaa cagctgggta caggtgaaaa
     6061 cacaccgcac cattggcgat gccgtcgctc atctcaaagg ccagggcatg cagattctgg
     6121 caacccatct ttctgataac gctgtcgatt tccgcgaaat tgattacact cgcccgacct
     6181 gcattttgat gggacaggag aaaacgggca tcacgcagga agcattggcc ctggcggatc
     6241 aggacatcat cattccgatg atcggcatgg tgcagtcgct gaatgtttcc gttgcctcag
     6301 ccctcattct ttacgaagcc cagcgtcagc ggcaaaatgc aggcatgtac ctgcgtgaaa
     6361 acagcatgtt gccggaagca gagcaacaac gcctgttgtt tgaaggcggc tatccggtgc
     6421 tggcgaaagt cgcaaaacgc aaaggcctgc cttatcccca cgtcaatcag caaggcgaga
     6481 tcgaagctga tgccgactgg tgggctacta tgcaggctgc agggtaagtg ccatgacagg
     6541 tcgcctgtta gatgctgtcc cactcagttc cctaacgggc gttggcgcag cacttagtaa
     6601 caaactggcg aaaatcaacc tgcataccgt gcaggatcta ctcttacacc ttcccctgcg
     6661 ctacgaagat cgcacccatc tctaccccat cggagaacta ctgccgggcg tttatgccac
     6721 ggtggaaggc gaagtgctga actgcaatat ctccttcggc ggtcggcgga tgatgacctg
     6781 ccagatcagc gacggttccg gcatcctcac catgcgcttt ttcaatttca gcgcggcaat
     6841 gaaaaatagc ctggcgacgg gccgccgtgt actggcttat ggcgaagcaa agcgcggtaa
     6901 atatggtgcg gagatgatcc acccggaata ccgcgtgcag ggcgatctca gcacgccaga
     6961 attacaggaa acgctcacgc cggtttatcc aacaacggaa ggcgtaaagc aggccacgct
     7021 gcgtaaatta accgaccagg cgctggatct gctcgacacc tgcgccattg aagaactcct
     7081 gccgccggaa ctgtcacaag gaatgatgac gctaccggaa gcgttgcgca ctttgcaccg
     7141 cccgccaccg acgctacagc ttagcgatct ggaaaccggg cagcatccag cgcaacgtcg
     7201 tctgattctg gaagaactgc tggcgcacaa cctcagcatg ttagccttac gtgccggagc
     7261 acagcgtttt catgcccagc cgctgagcgc caatgatgcg ctgaaaaata aactcctcgc
     7321 agccttaccg ttcaagccaa cgggcgcaca ggcacgcgta gtggcggaga tcgagcgcga
     7381 tatggcgctg gatgtgccga tgatgcgcct ggtacagggc gatgtaggtt ccggtaaaac
     7441 gctggtcgcc gccctcgccg cgctgcgtgc gattgcccac ggcaaacagg tggcattgat
     7501 ggcaccaacc gaattactcg ccgaacagca cgccaataac ttccgcaact ggtttgaacc
     7561 gctcggtatc gaagtgggct ggctcgccgg taagcagaaa ggcaaagcgc gactgtctca
     7621 gcaggaagcc atcgccagcg gtcaggtgca gatgattgtt ggtacgcacg ccatcttcca
     7681 ggaacaggtg cagtttaacg gtctggcgct ggtgattatc gacgaacaac atcgctttgg
     7741 cgtgcatcag cgtctggcgt tatgggagaa aggccagcag caaggcttcc atccgcatca
     7801 gttgatcatg accgccacgc cgatcccccg cacgctggca atgacggcgt atgccgatct
     7861 cgatacctcg gtgatagatg agctgccgcc aggccgcacg ccagtgacta cggtcgctat
     7921 tcctgatacc cgccgtaccg acatcattga ccgcgtgcgc cacgcctgca taactgaagg
     7981 tcgtcaggcg tactgggttt gtacgttgat tgaagagtca gagttactgg aagcgcaggc
     8041 ggcggaagct acctgggaag agttgaaact ggcgctacca gaattgaatg ttggcctggt
     8101 acacgggcgg atgaaaccag ccgagaaaca ggcggtgatg gcgtcgttta aacaaggtga
     8161 gctacatctg ctagttgcca ctaccgttat tgaagtcggt gttgatgtgc ctaacgccag
     8221 cctgatgatt atcgaaaacc ctgagcgtct gggtctggcg cagttacacc agcttcgcgg
     8281 gcgtgtaggt cgtggtgcgg tggcttctca ctgcgtgctg ctctacaaaa cgccgctctc
     8341 caaaaccgcg caaattcggc tgcaagtgct gcgcgacagc aacgacggtt ttgtgattgc
     8401 acagaaagac ttagagattc gcggaccggg tgaactgtta ggtacgcgtc agacgggtaa
     8461 tgctgagttt aaagtggcgg atttactgcg cgatcaggcg atgatcccgg aagttcagcg
     8521 cctggcgcga catattcacg aacgttaccc acaacaagca aaagccctga tagaacgctg
     8581 gatgccggag acggaacgtt actccaacgc atgaccagac tcatcacaag acaagtcacg
     8641 cagtaaatct tcaatctgga tagtgaggat atccattgcc tcatacgcat cctgtgcaat
     8701 tcgcgccgcc attgcgcctt ccggttcacc acgcagaatt tcagccgctg cctgtacgcc
     8761 agccacaatc atttcaggtg ttaacatttg gcgaagaaaa gcgcgattat cagaggacca
     8821 gccatcttta tggcctaatg gatgccccca gcctatccat tcttgcgacc ataacggatt
     8881 ggcttctatg ggataaatta gatgcgcggc atactgaata gcacacaccg cttttcctgg
     8941 cgtatccagc gcccaccagc gcgaccagag agaacgaatt aacggcgcgg ccccacccag
     9001 agtattgaac accgccagcc agcaaagaac gttattgaaa ccacgctcgt tatccatacg
     9061 ctgcaacgcg gtgtccagta aaaattgccg tacctgctgg cgttgggaag cattcatcat
     9121 ctcctgccag agatagggcc gacacaatgc cttcattaag gattcttcga agtgatcacc
     9181 ataaccgcca gctaaaacgg tttcatgcca ttgcgccagg cagagcggaa acagatagcg
     9241 aaacaactct atttgcatcg gttcgttata aacgagattg agtacatagt aagcactcaa
     9301 atcccattcg ctgatctgct gccactcttt agccgccagc gccttgagtt cggcatctgc
     9361 gtaatcaaat tccgtttccc atacacagag cactggcggc tcatcaccaa aaatatggcg
     9421 acagtgttca cgtagcgttt ccggttcctt ccgacgcttt ttcattttta accggcaaaa
     9481 atcggcaaca ttaaatacag cttaatcacc agcgcattga cgatatcgat aaagaacgca
     9541 ccgaccatcg gcaccaccaa aaacgccatg tgcgacgggc caaagcgttc agtgatcgcc
     9601 tgcatgttgg cgattgccgt tggcgttgca ccgaggccaa aaccacagtg acccgcagcc
     9661 agcactgccg catcgtagtt tttgcccatc atgcgccagg taacgaagat ggcatacaac
     9721 gccatgaaga tggtctgtac caccagaatc gccagcatcg gcagcgccag cgaagccagc
     9781 tcccacagtt tcagccccat cagcgccatc gccaggaaca acgacaggct tacgttaccc
     9841 agcacggata ccgcacgctc aaagacgcgg taaaagccca tcattgacag accgttgctc
     9901 agaatcacgc caacaaacag tacacagacg aaggtcggca gttcaaaagc agtgccagcc
     9961 aaaagttgcg caacaatttt ccccaccgtc aggcagatag caatcagcgc gatagtttca
    10021 atcagcacca gcgaggtgat catgcggccc acatccggct tttcaaacgc cgtcgggact
    10081 tcctggtcat ccggaatacc gttcggcgtg gtggagtgtt tcaccagata gcgcgccacc
    10141 ggaccgccaa tcaagccgcc cagcaccaga ccgaacgttg cacaggccat cgccacttcc
    10201 gtcgcattgg tgaagccata acgttcaatg aacaatttac tccacgcagc gcccgtaccg
    10261 tgaccgccgg aaagcgtaat agaaccggcc aacagcccca tcagcggatc aagccctaac
    10321 aggctagcca taccaatgcc aatggcattt tgcatcacca acagaccaac aaccacaatc
    10381 aagaagatgc caaccacacg cccaccggca cgcaaactgg caatgttggc gttcaggcca
    10441 atggtggcga agaaagccag cattaacgga tcgcgcaggg acatatcaaa gttgacttcc
    10501 cagcccatgc tttttttcag tactagtagc gccagcgcca ccaacaaacc acccgcaaca
    10561 ggttccggta tggtgtattt cttcaaaaag gagacggaat ggaccaactt acgcccgagc
    10621 aacaacgtca gcgttgcggc aacaagcgtt gctaaagtat cgagatgaaa catagttact
    10681 cctttgtatc cgcatcactc tttcatactc gtcatacttc atcgcgcagt ggcaacttca
    10741 tgaactatga cgggtatcga gttatgcttt cctgggtcaa attctcagct gttaatgaac
    10801 aaatggcatg gattttaagc agaaaaatac caaaagttat ataaaaaagt gcatttatct
    10861 catttaaata tttagatgag gatgtggcaa acgtttgctt ttctctttcg gtcagctaaa
    10921 atgcccgctt tgctaccacg ggattgtttt cgatgtctgt ttccaccctc gagtcagaaa
    10981 atgcgcaacc ggttgcgcag actcaaaaca gcgaactgat ttaccgtctt gaagatcgtc
    11041 cgccgcttcc tcaaaccctg tttgccgcct gtcagcatct gctggcgatg ttcgttgcgg
    11101 tgatcacgcc agcgctatta atctgccagg cgctgggttt accggcacaa gacacgcaac
    11161 acattattag tatgtcgctg tttgcctccg gtgtggcatc gattattcaa attaaggcct
    11221 ggggtccggt tggctccggg ctgttgtcta ttcagggcac cagcttcaac tttgttgccc
    11281 cgctgattat gggcggtacc gcgctgaaaa ccggtggtgc tgatgttcct accatgatgg
    11341 cggctttgtt cggcacgttg atgctggcaa gttgcaccga gatggtgatc tcccgcgttc
    11401 tgcatctggc gcgccgcatt attacgccgc tggtttctgg cgttgtggtg atgattatcg
    11461 gcctgtcgct aattcaggtt gggttaacgt ccattggcgg cggttacgca gccatgagcg
    11521 ataacacctt cggcgcaccg aaaaatctgc tgctggcagg cgtggtctta gccttaatta
    11581 tcctgcttaa ccgtcaacgt aacccttact tacgcgtggc ctcactggtg attgcgatgg
    11641 cggccggata tgcgctggcg tggtttatgg gcatgttgcc agaaagcaac gaaccgatga
    11701 cgcaagaact gattatggtg ccaacgccgc tctattacgg tcttggcatt gaatggagtc
    11761 tgctgctgcc gctgatgctg gtctttatga tcacttcgct ggaaaccatt ggcgatatca
    11821 cggcgacctc tgacgtttcc gaacagccag tgtccggtcc gctgtacatg aaacgcctga
    11881 aaggcggcgt gctggcaaac ggcctgaact cgtttgtttc ggcggtgttt aacaccttcc
    11941 cgaactcctg cttcgggcaa aacaacggag tgatccagtt gactggtgtt gccagccgct
    12001 atgtcggttt tgtcgtcgcg ctgatgttga tcgtgctggg tctgttcccg gcagtgagcg
    12061 gttttgtaca acacattcca gaaccggttc tgggcggcgc aacgcttgta atgtttggca
    12121 ccatcgccgc ctccggtgtg cgtatcgttt ctcgtgagcc gctgaaccgt cgggcgattc
    12181 tgattatcgc gctgtcgctg gcggttggtc tgggcgtgtc tcagcagccg ctgattttgc
    12241 agtttgcccc tgaatggctg aaaaacctgc tctcctccgg gatcgccgcg ggcggtatta
    12301 ctgccatcgt gctgaatctg attttcccac cagaaaaaca gtaattcact ggcggcggta
    12361 agataattac cgccgcagtc tcacctcttt caccattccc gccttgagga ttgcgcgtaa
    12421 atcgtgcata actcccttat gtgcatttca cgggatggaa gaccatgaaa tttattggga
    12481 agctgcttct ctacattctc atcgctctgt tagtggtgat cgctggcctc tattttcttc
    12541 tgcaaacccg ctggggagca gaacatatca gcgcatgggt ttccgagaat agcgactatc
    12601 atctggcctt cggggcgatg gatcaccgtt tttccgcgcc atctcatatc gtgctggaga
    12661 acgtcacgtt tggtcgtgat ggtcagcccg cgaccctggt ggcaaaaagt gtcgacattg
    12721 cgctaagcag tcggcaactg accgaaccac gccatgtcga taccatcctg ctggaaaacg
    12781 ggacgctgaa tctcaccgac cagaccgcgc cgctaccgtt caaagccgat cgtctgcaac
    12841 tgcgtgatat ggcgtttaat agcccgaata gcgaatggaa actgagcgcg cagcgggtaa
    12901 atggcggcgt ggttccgtgg tcaccagaag ccggtaaagt gctgggtacg aaggcgcaga
    12961 ttcagtttag tgccggatcg ctttcgctca atgatgttcc tgccaccaat gtactgattg
    13021 aaggcagtat tgataacgat cgcgttacgc tgactaacct gggtgccgac atcgcccgcg
    13081 ggacattaac cggaaacgcg cagcgtaacg ccgacggcag ctggcaagtg gaaaatctgc
    13141 gcatggcgga tatccgtcta caaagcgaaa aatcgctaac cgacttcttt gcgccattac
    13201 gctctgtccc gtcgttgcag attggtcgcc tggaagtgat cgatgctcgt ttgcaaggtc
    13261 cggactgggc ggtgaccgac ctcgatctca gcttgcgcaa catgaccttc agtaaagatg
    13321 actggcagac acaagaaggc aaactgtcga tgaacgctag cgagttcatt tatggttcgc
    13381 tgcatttatt tgacccgatt ataaacgcgg aattttcccc gcagggcgta gcgctgcgcc
    13441 agttcaccag ccgctgggaa gggggtatgg tcagaacgtc agggaactgg ctgcgtgacg
    13501 ggaaaacgtt gatccttgat gatgcggcaa ttgccgggct ggaatatacc ttgccgaaaa
    13561 actggcaaca gttgtggatg gaaacgacac ccggttggtt aaacagcctg caactgaaga
    13621 gatttagcgc cagccgcaat ctgatcattg atatcgaccc tgacttcccg tggcagctca
    13681 ccacgctcga tggttacggt gccaacctga cgctggttac cgatcataaa tggggcgtct
    13741 ggagtggctc ggcgaatctg aatgccgccg ccgcgacatt caatcgtgtt gatgttcgtc
    13801 gcccgtcgct ggcgctgacc gccaacagca gcacggtgaa tatcagcgaa ctgagtgcat
    13861 ttactgaaaa aggcattctg gaagccactg ccagtgtttc acaaacgcca caacgtcaga
    13921 cccatatcag cctgaatgga cgcggtgtgc cggtgaatat tttgcaacaa tggggatggc
    13981 ctgaattacc gttgactggc gacggcaata ttcagcttac cgccagtggc gatattcagg
    14041 ccaatgtccc gctgaaacct acggttagcg ggcaactcca tgccgtgaac gccgcaaagc
    14101 agcaagtgac tcaaaccatg aatgcgggcg tcgtttccag tagcgaagtt acatcgacag
    14161 agccggtgca gtaaaaatca atctcgccgg gtacgactca gacgtacccg gcattccatc
    14221 aatagattac aacgtaattg tcagcgcatt cccttgaggc gtcaccacca gcccctgctc
    14281 actttcagcc tgcgaaccac cttgcagacc atttactttc acaatattgc gcaagcacag
    14341 tgtccagttc ttcgcctcgc ccgcaccagt cacagtaatc gtgttgccag tacgcgccgc
    14401 tttcagggtg aaaagaacgg aaccgtcagc agcgggcact tcacagatgg cttcatgccc
    14461 gtcttgtaga ttaaagagct ggaatgccgt gccttcgtgc cacgcgtaat cgggacgttg
    14521 ctcgttgttg cccagcgcca gtagggtgtt atcacgcaca taaacgggca gactcaggaa
    14581 gctgtgctgc tgtttatgcc agcgactacc atcgagttca tcgttgtgcc acagatgtgt
    14641 ccagcgacct tccggcaagt agaactgcac atcgcccgct tcactgaaca ccggagcaac
    14701 catcacgttg tcgcctaaca tgtattgacg gtcaaggtaa tcacaagccg gatcgtccgg
    14761 gaactccatc atcatggccc gcatcatcgg cgtaccccgc gcgttcgcac gagcagcttc
    14821 acgatacaga tacggcatca tgcggcattt cagttgcgtg aagaagcgca ccacatcaca
    14881 ggactcatca tcgtaggccc acggcacacg ataagatttg ctgccatgta aacggctatg
    14941 gctggagagc aaaccaaacg cgcaccagcg tttgtaaacg tgcgccagag cggtattctc
    15001 aaagccgccg atatcgtggc tccagaaacc aaaacctgaa aggccaatag acaaaccacc
    15061 gcgcaggctt tccgccattg attcgtagtt agcgtaacag tcaccacccc agtgtaccgg
    15121 gaatttctgc gcaccaacgg aggccgagcg agcaaacaag acggcttctt cctcgccaac
    15181 ggtgtccttg agcacgttcc acaccagttc gttgtagatg tacgcataat ggttatgcat
    15241 tttctgcgga tcggaaccgt caaaccactg aacatcgatt ggaatacgtt cgccaaagtc
    15301 ggtcttaaag caatcaacgc ccatcgcgac cagacctttc agtttgtcgg cgtaccattt
    15361 gcaggcatcc ggattggtaa agtcataaat cgccagacct ggctgccatt tatcccactg
    15421 ccataacgaa ccgtccgggc gtttgagtaa atagcctttc tcttgtaact ctttaaagac
    15481 aggggatttt tgcccgatat aggggttaat ccagacgcag atttttagcc ctttcgcttt
    15541 caggcggcgg atcatccctt ccgggtccgg gaaagtcagc gggtcccact caaaatcgca
    15601 ccactggaag gctttcatcc agaaacagtc aaagtgaaaa acatgcagtg gcagattgcg
    15661 tcccgccata ccatcgataa agctgtttac cgtcgcttcg tcgtagttgg tggtaaatga
    15721 agtggttagc cacaggccga aggaccacgc gggcggcagc gccggacgac cagtaaaacg
    15781 ggtataacga tcaagtaccg ctttcggcgt cgggccgtcg ataacaaagt attcgagata
    15841 ttcactctca acgctgaact gcactttgga gactttctcc gatcccactt caaaagagac
    15901 gcattgagga tgattgacca gtaccccata gccacggtta gtcatataga acgggatatt
    15961 tttatacgcc tgttcagtac ttgtgccgcc gtcccggttc caggtctcta ccgtctggcc
    16021 attgcgcacc agggcagtaa agcgctctcc cagaccgtaa actgtttcgc caacgccaag
    16081 atccagccgc tcaaacatat aatttcgttg attattcgtg tcctgcacgt agccattatt
    16141 tttcacctga ctaccggtaa tacgttcgcc gttgcgcaga aaatccagtg accagaactc
    16201 acctttgctg acacgcgcgc ttaagttgcc acttttaaac tcagcgtaac gttctgtgtt
    16261 ttcgattgtg accttcacgt cctgcaaaat attgagcgga taatgaggac cgttattcag
    16321 cgccccctga aaatgctcaa tccgtacacc gacaatacct tcctgtgggg agaaaaagcg
    16381 cagcgtaaat aaaggcgtat caagctgcca ggttcgttca cgcacatcac ggggggcagc
    16441 atagaccacc atttcattac cctgctgttc aacctcgaac acctgaagcg ggtgaatcaa
    16501 attgaggcca ggttgaatca accagtttcc gtcgctaatt ttcattgccg ttccttagtt
    16561 ctgcacttct tgagaggtga attgttgctg gcaacggcgt ttgccctggg ccagctgttc
    16621 cataacggtt ttcagattgt gcgtcgtgag tgagtagtag cgtttagcga taatcgcgct
    16681 cagcaaataa cagatcgccg gaacaatcgt gaatagcgca ataatgatgc taatcgtggc
    16741 gctgttctgc gctttttctg ccgcatcata tccgccataa gccagcatcc agccgataag
    16801 agcgccgccg aaggccaacc ccagtttgag cacaaacagc gtgccagcaa aactgatccc
    16861 ggtcaggcgt ttaccattgc accactcgcc gtagtcgacg gtatcggaca tcattaccca
    16921 ctggataggt gtcaccagtt gatgcaatac accaatcacg aagatgaaga caaacatagt
    16981 gatgctggcc tgcataggaa caaagaacat cgcgaggcta atcactgcca gcagggcgtt
    17041 cgtccaccag aagatagtga ctttacattt ccagtcggtc agcggttttg ccagtgcgga
    17101 accaatcagg ttacccacgc aataagtggt gagaaaagcg acaaacactt ccggcgtacc
    17161 caaaatccat gtgacgtaat acatcatcgc tccaccgcgg acgcacaccg ccaggatatt
    17221 gaaaatggtt agtaaaccga caatccgcca ctggtcgttt tgccagatat cacgtaaatc
    17281 ttcccgcata gacgtcgttg taggtggtgc ttcaacgcgt tctttagtgg tgaagaaaca
    17341 gaatgccagc atcatgaatg ccaccacgga aaggaccgcg ataccgccct ggaaaccgag
    17401 tggtttatta tcaccgccaa ttaaattaac cagtggcatc atcagaacag tagagagcat
    17461 gcctcccgcg gtcgccagca caaaacgcca ggattgcagc gagatacgct gagtcgggtc
    17521 attggtgatt acaccaccca atgcgcagta agggatattg acgacggtat aaagtaaggt
    17581 aagtagggtg taagtaattg ctgcatagat cattttgccg ttcatactga gatctggcgt
    17641 gctataggcc agtacacaga cgatcccaaa tggcagtgcg ccacacagta cccacggacg
    17701 aaatttaccc cagcgagagc gcgttcgatc ggccaacaac cccatgcaag gatcggaaat
    17761 cgcatccagt gcacgagcga ccaaaaacat ggttccgaca aatccggcag gtatgccaaa
    17821 attatcggta taaaagaaca tcatatataa cattacgtta tcgaaaataa tgtggctggc
    17881 ggcgtctccc atgccataac caattttctc tttaacggac aacacttcac tcttcatctg
    17941 cttttccttg atgctatagg ggtgctgaga ccgataacgc attttgtaaa ccatccctgt
    18001 gagagcgaat attccgatat ctggttatta atttatgatt cttgttttat gtgatcgtgg
    18061 tagcgttaat tccgctcata tatcattgta aaatatgagt tttatatgaa ttataatgct
    18121 ttcgtgataa tacgctgcgt gtattaggcg gaaaaaactg atctggggga tgtagaaact
    18181 caaggaagta gctataatgc gccccgcctc catgtagcaa tcgaggcgcg gaagatcgtc
    18241 gtctccggtg aggcggctgg acttcaaatc cagttggggc cgccagcggt cccgggcagg
    18301 ttcgactcct gtgatcttcc gccagtttgc ctctcctgaa ctctcccgac atcaacaaag
    18361 ccccttataa tcccttatat atcaggtttt ctttgtatcc tgaggtcaaa cgactttaac
    18421 ccatatcaag ttgtttgtgg gggtgcattg ggggtatcag gttggttact ggcggaaatt
    18481 cccccaggag gatggattca tcaaaagctg gaggatgtca tggcgctaac cgatgttaaa
    18541 gtgaaaaccg cgaagccaaa agaaaggccc tataagctgg ctgatggcgg aggtatgtat
    18601 ctgctgatta acgctaatgg ttccaaatac tggcgtatga agtaccgctt cgccggtaaa
    18661 gaaaagatgc tgtctattgg cgtatacccc gatgtaacgc ttgccgacgc acgtgagaaa
    18721 cgcagtgaag ccaggaaaat acttgcggct ggtggcgatc cgggtgaggc aaaaaaagaa
    18781 gaaaaaatag cgctgcaaat gagtttgaaa aacactttcg aggccgttgc tcgcgagtgg
    18841 catcagacga aagccgatcg ctggtcactg cgctaccgcg atgaaataat cgacaccttt
    18901 gagaaagata tcttccccta catcggcaag ctacctattg ccgaaatcaa gccgatggag
    18961 ctattggaag cactacgtaa gatggagaaa cgtggagcac tggagaaaat gcgcaaggtc
    19021 cgccaacgtt gtggtgaggt gtttcgctat gcaattgtta ctggtcgagc cgactataac
    19081 cctgctcccg atcttgccag tgctttagct acaccgaaaa aagtacattt tccctttctt
    19141 actgccaatg aacttcccca cttccttact gatctggcgg gttataccgg aagtatcatc
    19201 actaagacag ctactcagat cattatgtta accggtgtac gaacgcaaga gttgcgtttc
    19261 gcgcattggg aggatattga ttttgaggca aaattatggg agatcccggc agaagtaatg
    19321 aagatgaaac ggcctcatat tgtgccgccc tctgagcagg ttattgcgct atttaagcaa
    19381 cttgaaccaa tctcaaaaca tcatcctctg gtctttatcg gtaggaacga tcctcgcaag
    19441 ccaattagta aggagagcat taaccaggtc attgaattgc tggggtataa gggaagactc
    19501 acagggcacg gtttcaggca taccatgagc acaattctgc acgaacaagg ctttaattct
    19561 gcctggattg aaatgcagtt ggctcatgtg gataaaaact ccatcagggg tacctataac
    19621 cacgcccagt atctcgatgg tcgccgtgaa atgatgcaat ggtacgctga ctacattgat
    19681 tcactttctg agctggcctg atgaatgaaa gttcggtgac acaaaaataa tgaataacca
    19741 tccagcccca acaggcggat g
//
LOCUS       DAZKFZ010000068        19649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000068 DAZKFZ010000000
VERSION     DAZKFZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            289..789
                     /gene="tssB"
                     /locus_tag="ACHOQW_002301"
     CDS             289..789
                     /gene="tssB"
                     /locus_tag="ACHOQW_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9573374.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            824..1048
                     /locus_tag="ACHOQW_002302"
     CDS             824..1048
                     /locus_tag="ACHOQW_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573375.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1099..2490
                     /gene="tssC"
                     /locus_tag="ACHOQW_002303"
     CDS             1099..2490
                     /gene="tssC"
                     /locus_tag="ACHOQW_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9573376.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2581..2994
                     /gene="tssE"
                     /locus_tag="ACHOQW_002304"
     CDS             2581..2994
                     /gene="tssE"
                     /locus_tag="ACHOQW_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9573377.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2998..4848
                     /gene="tssF"
                     /locus_tag="ACHOQW_002305"
     CDS             2998..4848
                     /gene="tssF"
                     /locus_tag="ACHOQW_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9573378.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4812..5894
                     /gene="tssG"
                     /locus_tag="ACHOQW_002306"
     CDS             4812..5894
                     /gene="tssG"
                     /locus_tag="ACHOQW_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9573379.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5919..7199
                     /gene="tagH"
                     /locus_tag="ACHOQW_002307"
     CDS             5919..7199
                     /gene="tagH"
                     /locus_tag="ACHOQW_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP9573380.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7196..7720
                     /gene="tssJ"
                     /locus_tag="ACHOQW_002308"
     CDS             7196..7720
                     /gene="tssJ"
                     /locus_tag="ACHOQW_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9573381.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7723..9054
                     /gene="tssK"
                     /locus_tag="ACHOQW_002309"
     CDS             7723..9054
                     /gene="tssK"
                     /locus_tag="ACHOQW_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9573382.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9059..9820
                     /gene="icmH"
                     /locus_tag="ACHOQW_002310"
     CDS             9059..9820
                     /gene="icmH"
                     /locus_tag="ACHOQW_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP9573383.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9829..12606
                     /gene="tssH"
                     /locus_tag="ACHOQW_002311"
                     /gene_synonym="clpV"
     CDS             9829..12606
                     /gene="tssH"
                     /locus_tag="ACHOQW_002311"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9573384.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLT
                     AELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKG
                     QDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLT
                     TINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLN
                     LFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADF
                     FKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSR
                     VTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQN
                     DESVTKDETVL"
     gene            12603..13346
                     /gene="vasI"
                     /locus_tag="ACHOQW_002312"
     CDS             12603..13346
                     /gene="vasI"
                     /locus_tag="ACHOQW_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP9573385.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13351..14763
                     /gene="tssA"
                     /locus_tag="ACHOQW_002313"
     CDS             13351..14763
                     /gene="tssA"
                     /locus_tag="ACHOQW_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9573386.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHAQLDIPELQRRGLQLLASESKDFRLLAHLLRTLQHAGDPLLALHLLTLYVEH
                     YWTVAAPQNMAHKKRFASQIIKRFETGIEGFSQNAATTQRDTLLGELAKLAQCWQSHN
                     VPELAQATDDLFALYQRTFNRAAPAPVPTPAASGSSPQTTVTSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14900..18334
                     /gene="tssM"
                     /locus_tag="ACHOQW_002314"
     CDS             14900..18334
                     /gene="tssM"
                     /locus_tag="ACHOQW_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP9573387.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18345..>19649
                     /locus_tag="ACHOQW_002315"
     CDS             18345..>19649
                     /locus_tag="ACHOQW_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9573388.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLK"
BASE COUNT         4575 a         5440 c         5464 g         4170 t
ORIGIN      
        1 attatataat tcaataagat acacaaaaaa ctctatattt aaattattgc cacaatacac
       61 ctgacgcgaa ttgcacaggt tcataaaatc tatttaacgc ttgcagaata cccctcttta
      121 cgatcgataa aaagtccttt tcttatttat tttgaagcgt aaaatgcggg gtgatgaagg
      181 agtggtgatt ttattttcac cgccatcaag acggcatttc cgatattttt tgcggtacga
      241 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
      301 tttgaaggca gcgtcgcgcc tcgcgaacgt attaatatat cgtatgtccc aaaaacagat
      361 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
      421 acgcaggaaa catcctcact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
      481 ggtgcagtga tggctgaagc agccattggc ctcaatttta ccgtccccgc cacgctgaag
      541 ggaagcacaa cggacgatga aatgaatgtg gcgctgaata tcaagtcact ggatgatttt
      601 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
      661 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
      721 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg ccaggtgagc
      781 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
      841 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
      901 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
      961 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     1021 gtcgcactgg cagcgcgtac ttcactgacg gcgaaaagcc ggtttcagaa acacagctaa
     1081 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     1141 cagcctgagc aggaatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     1201 ggttcggaag gttatgacgt tgcccgccag ggtgtgacag cctttattgc cagcatcctg
     1261 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     1321 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     1381 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     1441 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     1501 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     1561 ggcggtgaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     1621 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     1681 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     1741 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     1801 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     1861 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     1921 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     1981 taccgctggt gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     2041 gtgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     2101 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     2161 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     2221 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     2281 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     2341 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     2401 aacccgcctg cagacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     2461 gatgtagaag gcgaacccgg ctggtactag gttgcgctaa gcgtgagacc tcattttaaa
     2521 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     2581 atgatactgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     2641 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     2701 agaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     2761 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     2821 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     2881 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     2941 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     3001 gaatttgaag aacgctattt tcgggaagaa ctcgattacc tgcgccagct tagcaagctg
     3061 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc ggatccggat
     3121 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     3181 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     3241 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     3301 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     3361 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     3421 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     3481 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     3541 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     3601 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     3661 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     3721 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     3781 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     3841 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     3901 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     3961 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     4021 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     4081 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     4141 catcagatgg aatacagccg gaagcgggag gtggtgtact ggcatcaccg gaccaaaaca
     4201 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     4261 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     4321 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg taaaaatgcg
     4381 gctgttgctt cctttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     4441 ggcagcctgc actggtccct gctctccgcc atgaacctga attatctgtc attactggat
     4501 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca ccatccgcag
     4561 acggcgcggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     4621 gatcgcctgt ttacgggtat tcccgtccgg ggactggcct ccacgctgta tctgcacccg
     4681 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     4741 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     4801 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     4861 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     4921 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     4981 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     5041 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     5101 ggatgaccac tacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     5161 tcgatcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     5221 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     5281 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     5341 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     5401 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     5461 gctttgattt gtcagaagtg acgctgcaaa actggcaacg tcgcaaggtg gatattgaac
     5521 cggatcagca aaacagcctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     5581 gatcggtact gggcaacttt gttctgggta cacgagtacc cgatctcagc ggaaaattcc
     5641 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     5701 acttcctgcc actgacgacg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     5761 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     5821 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     5881 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     5941 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     6001 tgttcacaca gcagggaaat gtcggccatg cccccgaatg ccactggtcg gtacaggatc
     6061 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     6121 tacgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     6181 tgatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     6241 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     6301 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     6361 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa tggtttttct gccgatccac
     6421 tccaggcact tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     6481 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     6541 ttatggatct gccgcccatt tatgccagcc ctggcgatca taatgatgac atctctgcgg
     6601 cagaaatggc gcaacgccac cttgcggtca cccccttact gcgcggtctt ggcggctcgc
     6661 ttaccgtgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     6721 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     6781 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     6841 acgtgatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     6901 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcggcgatt gtggagtcgc
     6961 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     7021 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     7081 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     7141 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     7201 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     7261 gaaaaaaatc agcgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     7321 accttcggaa ataaccgtca ccctgctgac tgaaccggac accaatacca atgcggaggg
     7381 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgcaggc
     7441 agccgactat gaccagatag ccagtacgcc gctaccggat gtgctgggta aaaactatat
     7501 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cgataaagct
     7561 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     7621 atggaaacaa atcgagacgg tggaaggaac cggccaccat taccgcctgc tggtgcatgt
     7681 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     7741 aaggtgatgt ggcaggaagg gatgctgatg cgcccacacc atttccagca acagcagcgt
     7801 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     7861 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     7921 tcaggcacac tgcctgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     7981 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatttacct cgctctgccg
     8041 gtcgccagtg atgtgagaaa tgaaatcagc gacgggcggc gaatcgggcg ttaccggctg
     8101 aattatgccg atgtccggga tctgcattca gaagaaggcg acgcgcgaac actgacgctg
     8161 ggacaactga cgccgcgcat tatgagcggt gcagaagata tgagtgccta tattacgctc
     8221 ccgctttgcc gtatcagcga tcgccatgcc gacggctccc tgacgctgga tgacgatttt
     8281 atcccctcct gccagaatat tcaggtcagt aagaaactgc gtgtttatct caaagaggta
     8341 cagggggcca ttggcggacg ggcaagcgat ctggcaaacc gtattggctc tccggcgcag
     8401 agcggcatcg cggatgtggc ggaatttatg atgttgcagt tgcttaaccg taaccagacc
     8461 cggtttaccc atcgcgcccg ccgatcccag ctccacccgg aagatttcta ccttgatctt
     8521 gccgagttgc tgggtgaact gatgaccttt acagagccgt cacgcctgcc ctgcccgctt
     8581 gatgtgtatg atcatcatga cctgaccaaa acatttaaaa cactgttacc ggaagtcaaa
     8641 cgggcgctgc ataccgtact gtcgccaaga gcggtcaatc tgccgctgca tctgcgtgac
     8701 ggcatctggc aggccgatgt tcatgactcg gaactgctac agtctgccac ctttgtactg
     8761 gccgtggcag caaatatgcc tgtcgatcag atccagcgtc agtttatcca gcagtcgaaa
     8821 atttcctcgc cggaaaaaat ccgcaatatg gtcagtgtgc agatcccggg tattcctctg
     8881 cgcgcactga tggtggcccc ccgccagctt ccctaccatt ccgggttcag ctatttcgaa
     8941 ctcgacaaga gcggccaggc ctggacagaa atggctgccg ccggggccgt tgcactgcat
     9001 gtatccggca gtttcccgga tctgaacatg caactgtggg cgataagagg gtaagacgat
     9061 ggatgagggg tcactgtctc tgccaccatt taccggatac gacgaaaaaa gcctgcgcga
     9121 ctaccatctc gcgcttcatg gcaacagcct gaatccgatg atcgacgcgg cgacgccgct
     9181 actcggtatg gtaatgcgcc tttccaccat gaacagccag acgatgccgg agcatctttt
     9241 tgctcaggtg gtgacagatg ttcaggccgt ggagcaactg cttcaggaac agggctacga
     9301 accgggcgtg attatctcgt ttcgctacat cctctgcacg tttattgatg aagcggcgct
     9361 cggtaacggc tggtcgaaca agaacgagtg gatcaagcag tcattgctgg tccatttcca
     9421 caacgaagcc tggggtggcg agaaagtttt catcctgctg gagcgcctga tccgtgaacc
     9481 caaacgttac caggatctgc tggaatttct ctggctctgt ttttctctgg gatttcgcgg
     9541 gcgctacaaa gtggcagtac aggatcaggg ggaatttgag cagatctacc gccgtctgta
     9601 ccacgttctg cataaactgc gcggcgatgc ccctttcccg ctgttacacc aggacaaaaa
     9661 aacccaaggc gggcgttatc agctcatcag tcgcctgact gttaaacata ttttttgtgg
     9721 tggcgttgtc gtgctggcgt tgttttacct gttctatctg ttgcggctgg acagccagac
     9781 gcaggacatc ctgcaccagc taaacaaatt acttcgataa ggacgtttat gatccagatt
     9841 gatcttccca cgctggtaaa acggctgaac ctgttctccc gccaggcgct ggagatggcc
     9901 gcctcagaat gtatgagcca gcaggcagcg gaaattaccg tcagccatgt gcttattcag
     9961 atgctcgcca tgccacgcag tgacctgcgg gttattaccc gccagggcga tattggcatg
    10021 gaagagttgc gccaggcgtt gacggtagag aactacacaa ccgcccgttc tgcggacagc
    10081 tacccggcgt tttccccgat gctggttgag tggcttaaag agggctggct gctggcgtcg
    10141 gctgagatgc agcacagcga actgcgcggc ggcgtgttgc tgctggcctt gctgcattcg
    10201 ccgctgcgtt atataccgcc tgctgccgcc cggctgttga ccggcattaa ccgtgaccgt
    10261 ctgcaacagg actttgtgca gtggacacag gagtcggcgg aatcagtcgt gccggatgca
    10321 gacggtaaag gcgcaggcac actgacggac gcctctgaca ccctgcttgc ccgctacgcc
    10381 aaaaacatga cagaagacgc ccgtaacggc aggctagccc cagtactgtg ccgcgaccac
    10441 gaaatcgacc tgatgatcga cattctctgc cgccgccgta aaaacaaccc ggtggtggtg
    10501 ggcgaagcag gcgtgggtaa aagcgcgctg attgaagggc tggcgctgcg catcgtggca
    10561 ggccaggtgc cggacaaact gaaaaacacc gatatcatga cccttgacct gggcgcattg
    10621 caggccgggg catcggtgaa gggtgagttt gaaaaacgct tcaaggggct gatggcggag
    10681 gtcatttcct ctccggtgcc ggtcattctg tttatcgacg aagcgcatac cctgattggc
    10741 gcgggcaacc agcagggcgg gctggatatc tccaacctgc tgaaaccggc gctggcgcgt
    10801 ggcgaactga aaaccatcgc cgccaccacc tggagcgagt acaaaaaata cttcgagaaa
    10861 gacgccgccc tgtcgcgccg cttccagtta gtgaaggtca gcgaacccaa cgctgccgaa
    10921 gccaccatta ttctgcgtgg tctgtcggcg gtctatgaac ggtctcacgg cgtgctgatt
    10981 gacgatgatg ccttgcaggc cgcagcaacg ctgagcgaac gttatctctc cgggcgtcag
    11041 ttaccggaca aagcgattga tgtgctggat accgcctgcg cccgtgtggc catcaacctg
    11101 tcgtcgccgc cgaagcaaat ctcggcgctg accactctga gccaccagca ggaggcggaa
    11161 attcgccagc ttgagcgcga gcttcgcatc ggactgcgta ccgacacatc acggatgacc
    11221 gaggtgctgg tgcagtatga tgaaacgctg acggcgctgg atgaactgga agcggcttgg
    11281 caccagcagc agacgctggt ccgggagatt atcgcgctgc gccagcagtt actgggcgtg
    11341 gcagaggacg atgcggcgcc gttgccggac gcagatactg tggaggatac gcagccagag
    11401 tcagagtcag agtcagagtc agaacaggat aataccggtg ccgtaccggc tgatgagacc
    11461 gacagagaac agccggaaga gaccgctgaa acagtttccc cggtacagcg gctggcccag
    11521 ctcactgccg aactggacgc cctgcataac gaccggttgc tggtctcccc gcacgtcgat
    11581 aaaaaacaga ttgcggcggt gattgccgaa tggaccggcg tgccgctcaa ccgcctgtcg
    11641 cagaatgaaa tgtcggtcat caccgacctg ccgaaatggc tgggcgacac catcaaaggc
    11701 caggacctgg cgattgccag cctgcataag catctactga ccgcacgcgc cgacctgcgt
    11761 cgtccgggac gcccgctcgg tgcgttcctg ctcgctggcc ccagcggcgt gggtaaaacc
    11821 gaaaccgtcc tgcaactggc agagctgctc tacggcggtc gccagtacct gaccaccatc
    11881 aatatgtccg agttccagga gaaacacacc gtttcgcggc tgattggctc ccctccgggc
    11941 tatgtcggct atggcgaagg cggcgtgctg actgaagcca tccgccagaa accctactcg
    12001 gtggtgctgc tcgatgaagt ggaaaaagcg cacccggatg tcctcaacct gttctaccag
    12061 gcgtttgaca agggcgagat ggccgacggc gaaggccgcc tgattgactg taaaaacatc
    12121 gtcttcttcc tcacctccaa cctcggttac caggtgattg tcgaacacgc ggatgacccg
    12181 gaaaccatgc aggaagccct ctatccggtg ctggcggact tctttaaacc tgccctgctg
    12241 gcgcgaatgg aggtggtgcc gtacctgccg ctgtcgaaag agacgctcgc caccattatc
    12301 gccgggaaac tggcccgtct ggataacgtg ctgcgcagtc gctttggtgc agaagtggtc
    12361 attgaaccgg aagtgacgga cgaaatcatg agccgcgtca cccgcgcgga aaacggcgcg
    12421 aggatgctgg aatctgtcat cgatggcgac atgctaccgc cgctctcgct gctgctgttg
    12481 cagaaaatgg cggctaacac ggcgattgcc cggattcggt tgtcggcagt ggacggcgca
    12541 tttacggcag acgtggaaga tgctcagaac gacgagtccg tcacaaagga tgaaacggtt
    12601 ttatgagcag gttgcgcgtt ttccggcaga caggatggtt tatcgcgggc ctgatgacgg
    12661 gcttacccgc cactgccgcg cccgcagaag catcgtcaat ggccggtgtg gcggtcgccg
    12721 tcgccaccac gaccccgccg gatgcgacag ccacgttaca ggccatgcag tcctgtcggc
    12781 gggagtccgc ggcgctggag cggcttgact gctatgacca cctgctggca ccgctgtccc
    12841 cgtcagggtt tgacggggcg ctggtcaaag ccggttttgt gggcgaggcc tggacccgcg
    12901 ccacagagca ggaaaaacgc cgcgagggca acaccacaga actgctggtg acgcaggtgc
    12961 cgggggagcg cccgacggtg gtgataacca ccccggctat cggccatgtt ccgccgcgcc
    13021 cggtgctgat gttcagttgc gtggacaata tcacccggat gcaggtggcg ctgatgcacc
    13081 ctctggatgt ccatgatatc gccgtcactc tgaatgccga cagccgggca ttgcgcagcc
    13141 actggtttgt gcgcgagaac ggcaccctgc tggagtccag ccgggggctg tccgggattg
    13201 atgaaattaa gcaactgttc ggcgcaaaaa cgctgacagt ggataccggc gcagataacg
    13261 ccgcaggaaa gctgaccttt aacattgacg ggctggcaag ggcgattgcc cccctgcgcg
    13321 atgcctgcca ctgggcggga gaataaaaat atggcaatgg acttacgcga tccgaatgtc
    13381 tggatatcgc acctgctgga aaacctgccg gaagaaaaac tggcatcggc gctgaaagat
    13441 gacaacccgg actgggagta tatcgacggc gaaatcgtca agctggggtc tctggcccac
    13501 gctcagcttg atattcccga actacaacgc agggggctac agcttctggc ttctgaaagc
    13561 aaagacttca ggctactggc acacctgctc agaaccctgc aacatgccgg tgatccactg
    13621 ctggcactgc acctgctaac gctatacgtg gaacattact ggactgtggc cgcgccgcag
    13681 aatatggcgc ataaaaaacg ctttgccagc cagatcatta aacgttttga aacgggtatt
    13741 gaaggctttt cacaaaacgc tgccacaacg cagcgcgata ctctgctggg tgagctggcg
    13801 aaactggcgc agtgctggca gtcacataac gtcccggaac tggcacaggc taccgatgat
    13861 ctttttgccc tgtaccagcg tacgtttaat cgtgcggctc ctgctccggt ccccactccg
    13921 gcggcctccg gtagttcacc acaaaccacc gtcacgtctg aaagcggcgt gacgcaaccc
    13981 agtgctccgg ctccccaaat cgccatcgac agtcacgacg acaaagcctg gcgcgacacg
    14041 ctgttaaaag tggcggctat tttatgtgaa cgccagccgg actcgccgca gggctatcgc
    14101 ctgcgccgcc atgccctgtg gcaatccatc accagtacac cccaggcgga aagcgatgga
    14161 cgtaccccac tggctgcggt ctctgccgat atggtggcgg attaccagtc ccggcttgcc
    14221 agcgcggata tggcgctgtg gcaacaggtt gagaaaagcg tattgctggc tccttactgg
    14281 ctggacggtc actgtctttc tgcacagacg gcactgcgtc tgggttacaa acaggtggca
    14341 gacaccatcc gcgatgaggt catccgcttc cttgagcgtc tgccccagct taccgggctg
    14401 ctgtttaatg accgcacacc gtttctcagt gagcagacga aacaatggct ggctgcttcg
    14461 cccgacggca aagttgcacc ggttgcgcaa atcggtgagg aatcgcaggc agccagagcc
    14521 tgttttgctg ggcagggtct ggaggcggcg ctgcgatatc tggacatgct acccgaaggc
    14581 gatccccgcg atcagtttca ccgccagtac cttgccgcac agttgacgga ggaggcgggg
    14641 ctgatacagc ttgcgcagca acagtaccgg atgttgttga tgatagggag tcagatgatg
    14701 gtgtctgact gggagccatc attacttacg cagcttgaac aaaaattcac ggcagaacaa
    14761 taaaagagaa caggagtttt tgtgttcaga ttaccaacac cccgactact cagcggactc
    14821 aaatcagccc tgcgaccggc gatgccccgg tttaaagtct ccgctttctg gctgctgata
    14881 ctggcgtgga tttttctgct tgctgatact ggcgtggatt tttctgctgg tatggatctg
    14941 gtggaaaggc ccgacgtgga cgctgtatga agaacagtgg ctcaaaccac tggcgaaccg
    15001 ctggctggca acggcggcgt gggggattat tgccctggtg tggcttaccg tccgggtaat
    15061 gaagcgcctg caactgcttg aaaaacagca aaaacagcag cgcgaagagg ccattgatcc
    15121 gctcagtgtg gagctgaacg cccagcagcg ttaccttgac cgctggctgc tgcgcctgca
    15181 acgccatctc gacaaccgcc gtttcctgtg gcagttaccg tggtatatgg tcatcggccc
    15241 ggcgggcagt ggcaaaacga cactgctgcg cgaggggttt ccgtcagaca ttatttatgc
    15301 cccggagggc gcgcgcggca cagaacaacg cctgtacctt acgccccatg tcggtaaaca
    15361 ggcggtgatc tttgatatcg acggcacact ctgcgctccc gctgatgcgg atatcctgca
    15421 tcgccgtctg tgggaacatg ccctcggctg gctgaaagaa aagcgcgcgc gccagccgct
    15481 gaacgggatt attctgacac tcgatttacc cgatctgctg accgcagaca aacgccaccg
    15541 cgagcatctg ttacagacgc tgcgcagccg cttacaggat atacgccagc atcttcactg
    15601 ccagttaccg gtttacgtgg tactgacccg gcttgattta ttacagggtt ttgccgccct
    15661 gttccagtcc ctgaacagac aggatcgcga tgcgattctg ggcgtcacgt tcacccgccg
    15721 tgcccatgaa aatgatgact ggcgaacaga gttgaatgct ttctggcaga catgggtgga
    15781 tcgaatgaat ctggcgttgc cggatctgat ggtcacccag actcacaccc gcgcgtcttt
    15841 attcagtttt tcccgccaga tgcagggaag ccgtgaaccg ctggtgtcac tgcttgaggg
    15901 tctgcttgac ggtgaaaata tgaacgtgat gctgcgtggt gtctatctca cctcttcgct
    15961 tcagcgcggg cagatggatg atatattcac ccagtctgcc gcccgccagt accgactggg
    16021 caataaccca ctggcgtcct ggcctctggt ggacaccgcg ccttatttca cccgcagctt
    16081 gttcccgcag gcattactcg cagagcctaa tctggcaacg gagagccgcg cctggctgat
    16141 gcgttcccgt cgccgcctga cggttttctc tgccaccggc ggcgtggcgg cactgctgct
    16201 catcaccggc tggcatcact attacaacgg taactatcag tccggcatca ccgtgcttaa
    16261 gcaggccaaa gcctttatgg atgtgccgcc tccacagggg gaagatgact acggcaacct
    16321 gcaactgccg ctgctgaacc cggtacgcga tgccacactg gcctatggcg actggggcga
    16381 ccgcagccgt ctggccgata tgggactgta ccagggacga cgtatcggac cttatgtgga
    16441 acagacctat ctgcaactgc tggagcaacg ttacctgccc tcgctgttta acgggctggt
    16501 taaagcgatg aatgccgcgc cgccggagag tgaagaaaaa ctcgcggtgc tgcgcgtgat
    16561 gcgaatgctg gaggacaaaa gcggacgtaa caatgaggtg gtgaagcagt atatggcaaa
    16621 acgctggagc gaaaagttcc acggccagcg cgatatccag gcacaactga tgtcccatct
    16681 tgactacgcg ctggctcata ctgactggca cgcagagcgt caggcgggcg acggtgacgc
    16741 catcagccgc tggacgccat atgacaagcc cgtggtatca gcacagaaag aactgagcaa
    16801 actgcctgtc taccagcggg tttaccagag cctgaaaacg cgggcgctgg gcgttcttcc
    16861 tgccgacctc aatctgcgtg accaggtagg gccaaccttt gaccaggtgt ttacatctgc
    16921 cgatgacaac aaactggttg ttccacagtt tcttacccgt tacggcctgc aaagctattt
    16981 tgtaaaacag cgcgatgaac tggttgaact gacggcgatg gattcctggg tacttaacct
    17041 tacccgcagc gtgaaataca gtgacgccga ccgcgcggaa atccagcgcc agctcaccga
    17101 gcagtatatc agcgactaca ccgccacctg gcgggccggg atggacaatc tgaatatccg
    17161 caattttgag tccatcggac aactgaccgg ggcgctggag caggttatca gcggcgacca
    17221 gcctttgcag cgggcgctga ccgtgctgcg tgacaacaca cagccaggcg tcttttctga
    17281 aaaactctct gccaaagaac gggatgaggc cctggcagag ccggactacc agttactcac
    17341 ccgtctcggg catgagttcg ccccggaaaa cattaccctg gcagtacaga aagacaaaga
    17401 aagcacgatg caggccgtgt atcagcaact caccgagttg caccgctacc tgctggcaat
    17461 ccagaacgcg cctgtaccag ggaaatcggc gctgaaagcc gtgcagttac ggcttgatca
    17521 gaacagcagc gatccgatat tcgccacccg ccagatggca aaaacgctgc ctgctccgct
    17581 caaccgctgg gttggcagac tgactgacca ggcctggcat gtggtgatgg tggaggctgt
    17641 acattatatg gaagtggact ggcgcgacag cgtggtgaaa ccgtttaacg agcaactggc
    17701 aaataactat ccgtttaatc cgcgttctgc acaggatgcc tcactggatg ccttcgaacg
    17761 cttctttaaa ccggatggca tactggatac cttctaccag cagaacctga agctgtttat
    17821 cgataatgac ctgagtctgg aggatggcga taacaacgtc attattcgcg aagatattat
    17881 tgcgcaactg gaaactgcgc agaaaatccg tgacatcttc ttcagcaaac agaacggtct
    17941 gggaacatcc tttgccgtgg aaacggtatc gctttcaggc aataaacgcc gcagtgtact
    18001 gaaccttgac ggtcagttag tcgattacag ccagggccgt aactataccg cccatctggt
    18061 ctggcctaac aacatgcgcg aaggcaacga aagtaagctg acgctcatcg gcaccagcgg
    18121 caacgcgccg cgcagtatca gcttcagcgg gccgtgggcg cagttccgcc tgttcggggc
    18181 cggacaactg accggagtac aggatggcaa ctttaccgtg cgctttagcg tggacggtgg
    18241 cgcgatgacc taccgtgtgc ataccgacac ggaagataac ccgttcagcg gtgggttgtt
    18301 cagccagttt ggtctgtcag acacactgta ctgaggaggc agggatgaat agtaacgtac
    18361 tgacacaaac tatcgttacc ggcagtgacc cgcgcgggct gccggaattc agcgccatcc
    18421 gcgaggaaat aaacaaagcc agccacccgt cacagcctga gctgaactgg aaactggtgg
    18481 agtcgctggc gctggcgatt tttaaagcca acggtgtgga tttacacacc gccacctact
    18541 atacgcttgc ccggacacgg acacagggac tggcgggatt ctgcgaaggt gcggaactgc
    18601 tggcggcaat ggtaagccac gactgggata agttctggcc gcagggcggc ccggcgcgta
    18661 ctgaaatgct ggactggttt aactcccgaa ccggcaatat tctgcgtcag caaatctcct
    18721 ttgcggaatc cgacctacca ctgatctacc gcacagagcg ggcattgcag cttatctgcg
    18781 ataagctcca gcaggtggaa ctgaagcgcg ttccgcgcgt ggagaatctg ctctatttta
    18841 tgcagaacac gcgtaaacgg cttgaacccc agctgaagag taacactgag aacgccgcac
    18901 agaccacggt cagaacgctg atttatgccc cggaaacaca ggcatcttcc acaccagaag
    18961 cggtagtgcc tcccctgccc ggcctgcctg agatgaaagt ggaagtgcgc agtctgacag
    19021 agaatccccc acaggccagt gtgataaagc aaggcagtac ggtaagaggg tttatcgcag
    19081 ggatcgcctg ttcagtggct gtcgcctcag cattgtggtg gtggcaggtc tatccggtgc
    19141 agcagcaact gttacaggtt aacgacaccg ctcagggcgc agcaacggtg tggatggcct
    19201 cacctgaact cgaaaactat gagcgcaggc tgcaacaact tcttgatacc tccccggtac
    19261 agccgctgga aaccgggatg cagatgatgc gtgttgccga cagtcgctgg ccggaaagcc
    19321 tgcaacagca acaggcctcg acacaatgga atgaggcact caaaacccgc gcacagagta
    19381 gcccgcagtt gcgtggctgg ttgcagaccc gccaggactt acatgctttt gcagatctag
    19441 tgatgcagcg cgagaaagag ggactaaccc tttcctatat caaaaatgtc atctggcagg
    19501 cggagcgggg actggggcag gaaacacccg ttgagtctct gttgacgcag taccaggatg
    19561 cccgtgcgca gaagcagaat acagatgcgc tggaaaaaca aattaatgag cgactcgaag
    19621 gcgtgttaag ccgctggctg ctgctgaag
//
LOCUS       DAZKFZ010000069        19615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000069 DAZKFZ010000000
VERSION     DAZKFZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..252
                     /gene="yahH"
                     /locus_tag="ACHOQW_002316"
                     /pseudo
     CDS             1..252
                     /gene="yahH"
                     /locus_tag="ACHOQW_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahH"
     gene            302..1252
                     /gene="yahI"
                     /locus_tag="ACHOQW_002317"
     CDS             302..1252
                     /gene="yahI"
                     /locus_tag="ACHOQW_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP9573389.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1262..2644
                     /gene="yahJ"
                     /locus_tag="ACHOQW_002318"
     CDS             1262..2644
                     /gene="yahJ"
                     /locus_tag="ACHOQW_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9573390.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2943..3353
                     /locus_tag="ACHOQW_002319"
     CDS             2943..3353
                     /locus_tag="ACHOQW_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9573391.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            3607..4590
                     /locus_tag="ACHOQW_002320"
     CDS             3607..4590
                     /locus_tag="ACHOQW_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9573392.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            4639..6123
                     /locus_tag="ACHOQW_002321"
     CDS             4639..6123
                     /locus_tag="ACHOQW_002321"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9573393.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            6116..7087
                     /locus_tag="ACHOQW_002322"
     CDS             6116..7087
                     /locus_tag="ACHOQW_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9573394.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            7084..8040
                     /locus_tag="ACHOQW_002323"
     CDS             7084..8040
                     /locus_tag="ACHOQW_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9573395.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            8127..9176
                     /gene="yahK"
                     /locus_tag="ACHOQW_002324"
     CDS             8127..9176
                     /gene="yahK"
                     /locus_tag="ACHOQW_002324"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP9573396.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9419..10234
                     /gene="yahL"
                     /locus_tag="ACHOQW_002325"
     CDS             9419..10234
                     /gene="yahL"
                     /locus_tag="ACHOQW_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP9573397.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            10685..10894
                     /locus_tag="ACHOQW_002326"
     CDS             10685..10894
                     /locus_tag="ACHOQW_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573398.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10911..11582)
                     /gene="yahN"
                     /locus_tag="ACHOQW_002327"
     CDS             complement(10911..11582)
                     /gene="yahN"
                     /locus_tag="ACHOQW_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP9573399.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            11729..12004
                     /gene="yahO"
                     /locus_tag="ACHOQW_002328"
     CDS             11729..12004
                     /gene="yahO"
                     /locus_tag="ACHOQW_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP9573400.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(12102..13688)
                     /gene="prpR"
                     /locus_tag="ACHOQW_002329"
     CDS             complement(12102..13688)
                     /gene="prpR"
                     /locus_tag="ACHOQW_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP9573401.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            13927..14817
                     /gene="prpB"
                     /locus_tag="ACHOQW_002330"
     CDS             13927..14817
                     /gene="prpB"
                     /locus_tag="ACHOQW_002330"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP9573402.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            14973..16142
                     /gene="prpC"
                     /locus_tag="ACHOQW_002331"
     CDS             14973..16142
                     /gene="prpC"
                     /locus_tag="ACHOQW_002331"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP9573403.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            16176..17627
                     /gene="prpD"
                     /locus_tag="ACHOQW_002332"
     CDS             16176..17627
                     /gene="prpD"
                     /locus_tag="ACHOQW_002332"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP9573404.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            17667..19553
                     /gene="prpE"
                     /locus_tag="ACHOQW_002333"
     CDS             17667..19553
                     /gene="prpE"
                     /locus_tag="ACHOQW_002333"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP9573405.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         4759 a         4799 c         5317 g         4740 t
ORIGIN      
        1 gaacgcctta tccggcctac gaatggcgca agaatctgta ggcctgataa gcgtagcgca
       61 tcaggcattt gtcaccattg ccggatgcgg cgtgaacgcc ttatccggcc tacgaatggc
      121 acgagaattg taggcctgat aagcgtagcg catcaggcat ttgtcaccat tgccggatgc
      181 ggcgtgaacg ccttatccgg cctacgaatg gcacgagaat ttgtaggcct gataagcgta
      241 gcgcatcagg cagtctggcg ttggtcataa ccccatcacc ctctgtagcg gacataacaa
      301 catgaaagaa cttgtggtcg ttgccattgg tggcaacagc attatcaaag ataacgccag
      361 ccagtcgatt gagcatcagg cggaggcggt gaaagccgtg gccgatacgg tgctggaaat
      421 gctggcttcc gattacgaca ttgtgctgac ccacggtaac gggccgcagg tcgggctgga
      481 tttacgccgt gcggagattg cccacgagcg cgaagggctg cccttaacgc cgctggcgaa
      541 ctgtgtggca gatacgcagg gcggcattgg ctacctgatc caacaggcgc tgaacaaccg
      601 gctggcgcgt cacggcgaga aaaaagccgt caccgtggtg actcaggtgg aagtggataa
      661 aaacgatcca ggttttgccc atcccaccaa gcccattggt gcattcttca gtgaaagcca
      721 gcgtgacgaa ttacaaaagg caaaccctga ctggcgtttt gttgaagatg ctggacgggg
      781 ctatcgccgc gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac ccgccattaa
      841 ggcgctgatc caacaaggct ttgtggtgat tggcgcgggc ggcggcggaa ttcctgtggt
      901 gcgtactgac gcgggagatt accaaagcgt ggacgcggtt atcgacaaag atctctctac
      961 cgcgctgctg gcccgtgaaa ttcacgccga cattctggtg atcaccaccg gcgtcgaaaa
     1021 agtctgtatc cactttggta aacccgagaa gcaggcgctg gatcgggtgg atattgccac
     1081 catgactcgc tatatgcagg aaggacattt cccgcccggc agcatgttgc caaaaatcat
     1141 cgccagcctg acgttcctgg aacagggcgg taaagaagtg attatcacca cgccggaatg
     1201 cctgccagcg gcgctgcgcg gtgaaacggg cacccacatt attaaaacgt aaggacgtaa
     1261 gatgaaagaa aataatagcc gccgtgaatt tctgagccag agcggtaaga tggtcaccgc
     1321 cgccgcgctg tttggtacct ctgtgccgct cgcccatgcg gcggtctctg gcaccacaaa
     1381 ctgcgaagcg aacaacacca tgaaaatcac tgacccgcat tactatctcg acaacgtgct
     1441 gctggaaacc ggttttgact acgaaaatgg cgtggcggtg cagacccgca cggcgcgcca
     1501 gaccgtggag attcagaacg gcaaaattgt cgcgctgcgc gagaataagc agcacccgga
     1561 tgccacgctg ccgcactatg acgctggcga taagctgatg ctgcccacca cccgcgacat
     1621 gcatattcat ctcgacaaaa cgttttacgg cgggccgtgg cgctcgctca atcgcccggc
     1681 aggcaccacc atccaggaca tgatcaaact cgagcagaaa atgctgccgg agctgcaacc
     1741 gtacactcag gagcgggcag aaaaactgat tgatttattg cagtcgaaag gcaccaccat
     1801 tgcccgcagc cactgcaata tcgaaccggt ttccggcctg aaaaatctgc aaaatttgca
     1861 ggcggtgctg gcgcgacgtc aggcgggctt tgagtgtgaa atcgtcgcct tcccgcagca
     1921 cggtttgctg ctgtcgaaat cggaaccctt aatgcgtgaa gcaatgcagg cgggtgcgca
     1981 ttacgtcggc gggctggacc cgaccagtgt cgatggcgcg atggaaaaat ccctcgacac
     2041 catgttccag attgcgctgg actacgacaa aggcgtcgat attcacctgc acgaaaccac
     2101 tccgtcgggc gtggcagcca tcaattatat ggttgaaacg gtagagaaaa cgcctcaact
     2161 gaaaggtaag ctgaccatca gccacgcctt tgcgctggct acgctcaacg agcaacaggt
     2221 agatgaactg gcgcatcgca tggcggcgca gcaaatttct atcgcctcga cggtgccgat
     2281 tggcacgctg catatgccgc tcaaacagtt gcacgacaaa ggcgtaaaag tgatgactgg
     2341 cactgacagc gttatcgacc actggtcgcc ttatggtctg ggcgacatgc tggaaaaagc
     2401 caatctctac gcgcagctct atattcgtcc taacgaacag aacctctccc gttcgctgtt
     2461 tctagccact ggcgatgtat tgccgctgaa tgaaaaaggc gagcgtgtat ggccaaaagc
     2521 gcaggatgac gccagctttg tgctggtgga cgcctcctgt tccgccgagg cggtggcgcg
     2581 tatctcgccg agaaccgcaa cgttccataa agggcaactg gtgtggggga gtgtggcagg
     2641 ttgatgcgca tttgaaacgc ctgcaacggt gaatagtaag agatttaagc cccagggagt
     2701 ggggcgggaa tggtttgcta atgaatgatc ctgaacttta taatctcccg gcataatttc
     2761 caggggatta tttgagagaa atacacaaaa gtgagtttta acaaaggtat ttatcaggcc
     2821 ataaatgtga cctgcttcgg ataaaaaaga ttcagtatcg aaaaaccaat gatactcttg
     2881 ctgatggtta ccaacagtaa ttaagatgtg atttgttttt ccgtaatcgc aaaaatattg
     2941 ccatgaccat aaaagaaaaa ttctcacaaa aatatcccca tgcttccttt tgtacttttg
     3001 gcgattcagc cgcgttggcc gatcacctcg caacgttgat tgcgactggt gttaaaacag
     3061 cttcctgtgg ttcgctggcg ggatgtattg aagacaacgc atttccgatg attggtgagt
     3121 ataagattgt agaaaatagc cgtggtgagc ctgtatgtgt tatcagagtg attggcctgc
     3181 atttactgcg cttttctgat gtcaccgcgg agcttgcccg taaggaaggt gaaggtgacc
     3241 tcagtcttga atattggcgt aatgaacacc gtcgtttttt ccaggcagaa ggaagctatt
     3301 caccagaaat ggacgttatc tttgaagaat atgcccttat tgacgttgtg tagataacta
     3361 tttatctaca tgaaataagc tcacaaacat aaaatatcgc taattttgtt tattttgctg
     3421 ttttttacgt gatgaatgtc acacttatta ttgctgcccg ttttgtccat atttataacc
     3481 atatttttaa tagctgcctg aaatagagct gtttattctg cataaaaata ttcgaaaatt
     3541 cttattctct ccagcgcgct aatttgggcg aacacttcct gactaccctg caatgaggct
     3601 gaaatgatga ataaacgttt tgttatcaat atggtgtctt cgctgttatt aggcgctgcg
     3661 ctaatttctg cgccgctcca ggcagcggaa aaagtcgtgg tgaatatttc taaagtcgat
     3721 ggtatgccgt ggtttaatcg catgggcgaa ggtgtggttg aggcgggtaa agcctttggt
     3781 gttaatgcct cacaggtggg gccttcaagt actgatgctc cgcaacaagt taaaattatt
     3841 gaggatttaa tcgcccgtaa ggttaatgca attactatcg tgccaaacga tgccaacgtg
     3901 ctggaaccgg tattcaaaaa agcgcgagat gccggaattg tggttctgac caacgaatcc
     3961 cccggtcagc caagtgctaa ctgggatatc gaaatcattg ataacgaaaa atttgccgct
     4021 gaatatgttg agcatatggc gaaacgcatg ggtggcaagg gcggctatgt gatttacgtg
     4081 ggtagtttga ccgtgccgca gcataactta tgggctgact tgctggtgaa ataccagaaa
     4141 gagcattacc cggatatgca tgaagttacc cgccgcatgc cggttgccga gagtgtggat
     4201 gtctcacgtc gtactacact ggacctgatg aaaacctatc ctgacttgaa agcggttgtc
     4261 tcctttggtt caaacggtcc gattggcgcg ggacgtgcgg tgaaagagaa acgggcgaaa
     4321 aataaagtcg cagtctatgg catgatgatc ccttcacagg cggcatcact gattaaaagt
     4381 ggtgatatca ccgaagggat tacttacgat ccggcctctg caggttacgc actcgcagcc
     4441 gtcgccagca cgttgctgaa gggtgaagag atcaagcctg gattagaaat gcagaatctg
     4501 ggtaaggccg acgtagatat ggataagcga attatccgtt tccataaggt attactggtc
     4561 aacaaagaca atatcgactc actttattaa tccatttctt cggggcgaaa gccccgattc
     4621 ttattgccag gggatgttat ggaaaccttc ctttcccttc gtcatatcaa caaaacgttt
     4681 catgccacgc gagcgctgcg cgatgtttct ctcgacttta tgtcgggtga agtgcactgc
     4741 cttgccgggc aaaacggctg tggtaaatcg acgttgatta aaattatgtc cggtgtttat
     4801 cgcccggatg agggagcgga aattacgctt ggtgggaaaa actggtcaaa gctgacaccc
     4861 gccgcttcgg tggcgcaggg gattcaggtg atttatcagg acctctcttt atttcctaac
     4921 ctgagtgtct gggagaatat cgccgtgaat cactatcacc acggtctgtt tgttaaccgc
     4981 cgtcgtctgc gtgaggtggc gcaggcggca atgactagta ttaacgtcac attgccactg
     5041 gatacgctcg tttctgagct ttcaattgcc cgctgtcagc tggtggcaat ttgccgtgca
     5101 ctggcgcagg acgcacggtt gatcgtgatg gatgaaccaa ccgcttcgct gacccatcag
     5161 gaagtgcaag ggttattaca ggtggtgcat caattgcgtg aacgcgggat ctgcgtggtc
     5221 tttgtcagtc atcgtctgga agaagtgatg gaagtttccg accgtatttc agtgctgaaa
     5281 gatggtgagc tggtcgggac ttttccggct gcagagatga ccacaaaaca actcggtttc
     5341 ctgatgaccg gccaggagtt tgaatatcag gtgcgagagt tgtggcaggg aaaatccagc
     5401 accccggtgc tggaggtgcg taatttaagt cgtcatgggg aatatctaaa tattaacttg
     5461 cgggtggaag cgggcgaagt ggtatcgatt gttggcctgc ttggtgcggg gcgcacggaa
     5521 ttatgtctga gtctgtttgg tatgacccga ccagatgctg gcgagatcct catcaacggc
     5581 cagctggtta cactgcatag taaccaggac gccatccgcc acggtattgg ttatgtttcg
     5641 gaagatcgca tgtcgcgcgg tttggtgatg gcgcaatcta ttgaagacaa catcattagc
     5701 actgtttttc acaaggtaaa agatcgcttc ggctttctga gtgaaactaa agtgcgtgac
     5761 ctggtggaca ggctaatcaa ggcattgacc atcaaagtct ctgatcctca tcttccggtg
     5821 aatacgcttt ctggtggtaa cgcgcagcga gtatcgatcg ccaaatggct ggcgattggg
     5881 ccacgactgt tgattctcga tagcccgaca gtcggtgttg atatcgccaa caaagcagga
     5941 atatacggca ttatcagcga tctggcggct catggcattg cggtattgat gatctgcgat
     6001 gaaattgaag aagcgtggta tcaaagccac cggattttgg taatgcaaaa aggtcagatc
     6061 acccatagct tcctgcctga cagcagttct caggccagga ttgcggaggt ggtaaatggc
     6121 tgaactgaaa aaacgccatg aattctggct ggcattactg attgtggtgc tgtttgttgg
     6181 ccttgcctgg cgcagcgacg agtttctgac attcggtaat ttgtacgatc tcgccaataa
     6241 ctatgccatg ttgactattc tcgcctgtgg tttgtttgtg gtgttgattt ccggtggaat
     6301 tgatatttcg tttccagcaa tgaccatcat tgcgcaatac ggcatggtgc tgttgctgca
     6361 aaaaattggt ggcaacttcg ctgtcgcgtt tgcactggca ggctgcatcg gcattttact
     6421 tggcttaatt aacgccttac tggttaatcg cctacgggtg ccttctatca tcatcactat
     6481 ctcgacgctg aatattttct atggcctgct gttatggttg agtaaaggtg tgtggctgta
     6541 cgactttccg ccgtggtttg agcagggggt tatgttgttc aagtacaccg atgctgatgg
     6601 ctatgactat ggccttggtc tgccgctgat cgccatgatt acggtggtgc tgctgacagc
     6661 gtttatcatg aatttcacca gtgtagggcg caaaatttat gcccttggcg ggaaccgcga
     6721 atcagccagt cgcatcggct ttagcgtgct gaaactgcaa cttttcgtct acggctatat
     6781 gggattgatg tctggcgctg cgggtgtagt gcagtcgtgg acggtgatga ctgtcgcccc
     6841 cgattctctt ctgggttatg agctgacagt actggctgcg gtggtgcttg gcggcactag
     6901 tttgctcggc gggcgcggca cgttaactgg tactttgctc ggcgtggtgt tgttggcagt
     6961 gatgcaaaac gggctaaatt tattgggagt ctcgtcttac tggcaaacat tgatcaccgg
     7021 catcatcatc gttgccagca ttagcgccac ggcgtggagt cagcatcaga accggagcct
     7081 gctatgaaaa aatcgtggcg taataacgtt gagttttact tgatcggcct gctggtgcta
     7141 actgttgccg cgttcagcat tactatgccg gagattttct ggtcgataag taatttccag
     7201 tccgtcgctt cgcaaatgcc cgtgctgggc attctggcgt tggctatggc ggtgaccatg
     7261 ctttgcggtg gcattaactt gtcaattata gccactgcta acgcctgttc actggtgatg
     7321 gcgtgggtgg cgactcagta tccaccgggt attgctacgg ttgtcgctac gttactggcg
     7381 ggcgctggcg cggcggtgat aatcggttta tgtaacggcg tattgattgc tggtattcga
     7441 gtgttgccaa ttcttgcaac gcttgggatg atgacgttgc tcaaagggat aaatattctg
     7501 gtgacaggtg gtagcgccat tgccaattat ccctcatggg tgctgtggtt gaatcatgcg
     7561 caatggttcg gtattccgct accgatgtgg ttgtttgccg cagtggcact ggggctgtgg
     7621 atattgctgg agaaaacgcc gctcggtaaa gccatttacc tgattgggtc taacgaacga
     7681 gcaacacttt actccggcat taacactcgt cgggtgctga tttgggttta tgtcatttcc
     7741 gccttgcttt gtgcggtagc ggcatttttg atgatgtcga aactcaactc ggctaaggcc
     7801 tcttatggcg aatcgtactt gctggtgtca atcctcgcgg cagtactcgg cggggttaat
     7861 ccggacggcg gcagcgggcg gataattggt atggtgctgg cgctgtttct tctgcaaatt
     7921 attgaaagcg gcttcaatat tctcggtata agcccctacc tgacgatggc cttatgggga
     7981 acgttgctgc tctgtttcat tcaggctcgc ggcatgttgg ggctggatcg ggtggtttaa
     8041 atattggcta tattcaatgg acgcgttttg ccgcgatgac atatcaggcg tcgccaaata
     8101 cacatagcta atcaggagta aacacaatga agatcaaagc tgttggttca tattccgcta
     8161 aacaaccact tgaaccgatg gatatcaccc ggcgtgaacc gggaccgaat gatgtcaaaa
     8221 tcgaaatcgc ttactgtggc gtctgccatt ccgatctcca ccaggtccgt tccgagtggg
     8281 cggggacggt ttacccctgc gtgccgggtc atgaaattgt ggggcgtgtg gtagccgttg
     8341 gtgatcaggt agaaaaacat gcgccgggcg atttggtcgg tgtcggctgc attgtcgaca
     8401 gttgtaaaca ttgcgaagag tgtgaagacg ggctggaaaa ctactgtgat cacatgaccg
     8461 gcacctataa ctcgccgacg ccggacgaac cgggccatac tctgggcggc tactcacaac
     8521 agatcgtcgt tcatgagcga tatgttctgc gtattcgtca cccgcaagag cagctggcgg
     8581 cggtggctcc tttgttgtgt gcagggatca ccacgtattc gccgctacgt cactggcagg
     8641 ccgggccggg taaaaaagtg ggcgtggtcg gcatcggcgg tctgggacat atggggatta
     8701 agctggccca cgcgatgggg gcacatgtgg tggcatttac cacttctgag gcaaaacgcg
     8761 aagcggcaaa agccctgggg gccgatgaag ttgttaactc acgcaatgcc gatgagatgg
     8821 cggctcatct gaagagtttc gatttcattt tgaatacagt agctgcgcca cataatctcg
     8881 acgattttac caccttgctg aagcgtgatg gcaccatgac gctggttggt gcgcctgcga
     8941 caccgcataa atcgccggaa gttttcaacc tgatcatgaa acgccgtgcg atagccggtt
     9001 ctatgattgg cggcattcca gaaactcagg agatgctcga tttttgcgcc gaacatggca
     9061 tcgtggctga tatagagatg attcgggccg atcaaattaa tgaagcctat gagcgaatgc
     9121 tgcgtggtga tgtgaaatat cgttttgtta tcgataatcg cacactaaca gactgaaaaa
     9181 attaataaat acgctgtggt ttaacatatt aacttcgctc tccacttaac tttttagtta
     9241 aggagagcga aataatatca aagtagcagt aaaacctata acgtaaattc aaattgttaa
     9301 attaactccc tccagtacct aatacttcac acgttagtta tgagcgattt ctgatagtgc
     9361 ctggtttaat cagagcttta ttatctgcga cgtttatttt tatttaagag agagcgcgat
     9421 gatatcatta aaagctccgc acaataattt gatgccatat acgcaacaaa gcatacttaa
     9481 tacggttaaa aacaatcagt taccagagga tatcaaaagc tccctggttt cctgtgtcga
     9541 tatattcaag gttttgatta aacaatatta tgattatcct tatgattgtc gtgatgattt
     9601 agtcgatgac gacaaactca ttcatctcat ggctgccgta cgagactgtg agtggtcaga
     9661 cgataacgca ctcaccataa atgtgcagtt taatgatttc cctggatttt atgactggat
     9721 ggattaccct gatcacccgg ttaagtttgt ttttcgcata cttgaaaatc aaaaagggac
     9781 agtttgggta tatgaccagg atgatgcgtt tcttgatatt aaagcaaatg tgcaggctgg
     9841 acgctttacc gggcttaaga aattagtgca gtttatcaat tcagtgcgaa cagattgcaa
     9901 atgtattctt cttgaacacc atatgccttt gctgagactt ttccctaaag ggaaagagtg
     9961 tatgcatgtg gaaaaatggc ttcgcgaaat gtcgtctatt ccggagacag acgcgcccat
    10021 taagcaggcg cttgcgcatg gattattatt acacttaaaa aatatctacc ctgtttttcc
    10081 tgaatcactg gtgatgttat tgctctctgt tctggacgta aaaacataca gagatgatgc
    10141 acgattaaat gaagggatat ctaatagagt ccaggaatta ggtgacagat attatcctgt
    10201 aaataagcac gttaaaatcc gctacactct ttagttcaga aagggttcca cctgcaagtt
    10261 gttattctac ctgagagtga atcgcaatgc aggtaatcat ttcatctgta tatctgtatg
    10321 ccaactggca ttccatctct tttttcgccg atattttgtt gtgatcggcg acacttcgct
    10381 taaaaaagca ccagtagtgg tttcgcagcc atgcggtgta taaaaaaaat gatctcatgc
    10441 agatgttttg tgaatgtgtg ggttgacatt catatgaaaa aaatcataat tccatcatgt
    10501 ttgtgtgggg tcttttctgt atcttacgca tcgcactcaa gcctgacaga aaatatgctg
    10561 taaggctcat atcaaaaacc gccattagct catcaggaag agcagacgac aaccaggatt
    10621 gttgtatggt acggggttcg aggccccgat ggcggtccaa cttttcaaaa ctcttctgaa
    10681 ttagatcccc ttattatcat ccctgcaaag tggaacattg ccattgtttg gctattcagg
    10741 acgggtcaga ccaatgaaaa aagcccacac aggggagagt gggctgaaat gggaagctaa
    10801 agactcaagt aaacttatcg gaaataagga ccacgcatta cgggggctat catcgccgat
    10861 ggtcgggatt cggcaaatca ggttgattac gtagagagta aattatctgc tcaccgctgc
    10921 gtcacccctt cgtaaatcag gcgtagcgcg aatacaccaa taattgcacc aataacccga
    10981 ctggcaacgc gttgcatacg cccataagca cgacgcacag cgggcaaaga aaacgcctga
    11041 ctaagaaaaa ctcgccagat aattgatgcg agcacaatcc ccgcccaggc cattaaacgt
    11101 gcccatgttg gtgtttcggc atttaatgtt actgagaaaa tactaataaa aaataaaacg
    11161 gtttgtgggt tagagagatc ggtaattaat ccacggcgaa aaaagacata ccagggggcg
    11221 ttaatcggtt gttgtagtgt gctcatttgc ggtgttgact ggcggcgcat gctgcaccac
    11281 gcaaaccata agagataagc gccgccgacg attctgataa gcgaaaaaat ctcctcacac
    11341 tgcgtaatta gcgttgcaag accaaacaaa cccaacccgg aataaaatgc atcgcccagc
    11401 gccacgccca gtccggtcag cactcctgcg cgtcgaccgg aagccaggct ggtttgtact
    11461 accacaaaaa gattggctcc cggattaaaa aaagtaatca cgaacagtcc tacggtcagg
    11521 taaacggcat gcaaaggatc catagtgatt tcatccataa ataagtgaac taactgcatc
    11581 atatttctac caaaaataat cgggtgcgag agagatcaca aagtgtctta tttccggtta
    11641 ctggcgttta tgccctgact gaactaatta ttaatcaacc caataatgtc ggtgggtgat
    11701 agtgtgataa caactctgga gccgtaatat gaaaataatc tctaaaatgt tagtcggtgc
    11761 gttagcgttt gccgttacca atgtctatgc cgctgaattg atgaccaaag cggaatttga
    11821 aaaagttgaa tcgcagtatg aaaaaatagg tgatatttca accagtaatg aaatgtcgac
    11881 tgcagatgca aaagaagatt taatcaaaaa agccgatgaa aaaggggctg atgtgttggt
    11941 actgacctcc ggtcaaactg acaataagat ccacggcacg gcggatattt ataagaagaa
    12001 gtaattctga atcctatgta aacatccccg atgcgtaagt ttatcggtga tcatctattg
    12061 aaatttatgc cggataaagc gttcgcgctg cattcggcag ttcagctttt cagccgccgc
    12121 cagaacgtcg tccggctgat gcctaaataa ttcgccgctg ctgttttatc gccattaaat
    12181 ttctccagtg cctgttgtgg tgtcagcaag cgtggagcgg gaattttcgc cgactcgcgc
    12241 gccagttccg gcagtagcag ctgcaaaaac tgcggcgtta aatccggcgt cggttccaca
    12301 cttaaaaata gcgccagtcg ctccatcata ttgcgcagtt cacgaatatt acctggccag
    12361 tcgtagtgca ccagcacggt ttcgcttgcc tgtaatccct ggcgtaatgc ggcagaaaat
    12421 ggggcggaga gcgccgccag agacactttc aaaaagcttt ccgccagcgg aagaatatct
    12481 gccacccgct cgcgcagtgg tggcaattgc agacgcaaaa tactcagccg ataaaacaga
    12541 tcacggcgaa actgcccttg ccgcatatct tcttccagat tgcagtgagt ggcgctaatg
    12601 acccgtacat ctaccggaac aggctgatgc ccgccgacgc gggtgacctc tttttcttcc
    12661 agcacccgta acaggcgagt ctgcaaaggt agcggcattt cgccaatctc atccagaaac
    12721 agcgtgccac cgtgggcaat ttcgaacagc ccggcgcgac ctccgcgtcg cgagccggta
    12781 aacgcccctt cctcatagcc aaacagctcc gcttccagca gcgattcggc aatcgccccg
    12841 cagttgacgg caacaaacgg atgcgacttt ttgccctgtc gcgcatcgtg gcgggcaaaa
    12901 tattcccgat gaattgcctg ggccgccagc tctttgcccg tccccgtttc cccctcaatc
    12961 aacaccgccg cactggagcg ggcatacagc aaaatagtct gccgcacctg ctccatctgt
    13021 ggtgattgac cgagcatatc gcccagcacg taacgagtac gcagggcgtt gcgggtggca
    13081 tcgtgagtgt tatggcgtaa cgacatgcgc gtcatatcca gcgcatcgct gaacgcctgg
    13141 cgcacggtgg cggcggaata gataaaaatt ccggtcattc cggcttcttc tgccagatcg
    13201 gtaatcagcc ccgcgccgac caccgcttcg gtgccgttag cttttagctc gttaatctgc
    13261 ccgcgtgcgt cttcttcggt aatgtagcta cgttgatcga ggcgcaaatt aaaggttttt
    13321 tgaaacgcca ccagtgccgg aatagtttcc tgataagtga caacgccgat agaagaggtg
    13381 agttttccgg cttttgccag tgcctgtaac acatcgtagc cgctcggttt aatcaaaata
    13441 actggcactg acaggcggct tttcaggtac gcgccgttag agccagcggc gatgatggcg
    13501 tcacagcgtt cgtttgccag tttcttgcgg atgtaggtca ctgctttttc aaagccaagc
    13561 tgaatagggg taatgttcgc caggtgatca aactcgaggc tgatatcgcg aaacagctcg
    13621 aataagcgcg ttacagatac cgtccagata accggtttgt cgtcattcag ccgtggtgga
    13681 tgtgccatag cgcaccgcaa agttaagata ccgaatattg ggtttagtct tgtttcataa
    13741 ttgttgcaat gaaacgcggt gaaacattgc ctgaaacgtt aactgaaacg catatttgcg
    13801 gattagttca tgactttatc tctaacaaat tgaaattaaa gatttaattt tattaaggca
    13861 attgtggcac accccttgct ttgtcgttat caacgaaaat aacaagttga taacaaagga
    13921 tgggctatgt ctctacactc tccaggtaaa gcgtttcgcg ctgcactgac taaagaaaat
    13981 ccattgcaga ttgttggcac cattaacgcc aaccatgcgc tgttggcgca gcgtgccgga
    14041 tatcaggcaa tttatctctc cggcggtggc gtggcggcag ggtcgctggg gctgcccgat
    14101 ctcggtattt ctactcttga tgacgtgttg acagatattc gccgtatcac cgacgtttgt
    14161 tcgctgccgc tgctggtgga tgcggatatc ggttttggtt cttctgcttt taacgttgcg
    14221 cgtacggtga aatcgatgat taaagccggt gcggcgggat tgcatattga agatcaggtt
    14281 ggtgcaaaac gctgtggtca tcgcccgaac aaagcgatcg tctcgaaaga ggagatggtg
    14341 gatcggatcc gcgcggcggt ggatgcgaaa accgatcctg attttgtgat catggcgcgc
    14401 accgatgcgc tggcggtaga ggggctggat gcggcgatcg agcgtgcgca ggcctatgtt
    14461 gaagcgggtg ccgagatgtt gttcccggag gcgattaccg aactcgccat gtaccgccag
    14521 tttgccgatg cggtgcaggt gccaatcctc gccaacatta ccgaatttgg cgccacgccg
    14581 ctgtttacca ccgacgaatt acgcagcgct catgtcgcga tggcgctgta cccgctttca
    14641 gcgtttcgcg ccatgaaccg cgccgccgaa catgtctaca acgtcctgcg ccaggaaggc
    14701 acgcagaaaa gcgtcatcga caccatgcag acccgcaacg agctgtacga aagcatcaac
    14761 tactaccact acgaagagaa gctcgacgac ctgtttgtcc gtagccaggc gaaataaaaa
    14821 acgcccgttg gttgtattcg acaaccgatg cctgatgcgc cgctgacgcg acttatcagg
    14881 cctacggggt gaactgtagg tcggataaga cgcatggcgt cgcatccgac aacaatctcg
    14941 accctacaaa tgataacaat gacgaggaca atatgagcga cacaacgatc ctgcaaaaca
    15001 gtacccatgt cattaaaccg aaaaaatctg tggcgctttc tggcgttccg gcgggcaata
    15061 cggcgctctg caccgtgggt aaaagtggca atgacttgca ttaccgtggc tacgatattc
    15121 ttgatctggc ggaacattgc gaatttgaag aagtggcgca tctgctgatc cacggcaaac
    15181 tgccgacccg tgacgaactc gccgcctaca aaacgaaact gaaagccctg cgcggtttac
    15241 cggctaacgt gcgtaccgtg ctggaagcct taccggcggc gtcacacccg atggatgtta
    15301 tgcgcaccgg cgtttccgcg ctcggctgca cgctgccaga aaaagagggg cataccgtct
    15361 ctggcgcgcg ggatattgcc gacaaactgc tggcgtcact tagctcgatt cttctctact
    15421 ggtatcacta cagccacaac ggcgaacgca tccagccgga aaccgatgac gactccatcg
    15481 gtggtcactt cctgcatctg ctgcacggcg aaaagccgtc gcaaagctgg gaaaaggcga
    15541 tgcatatttc gctggtgctg tacgccgaac acgagtttaa cgcctccacc tttaccagcc
    15601 gggtgattgc gggcactggc tctgatatgt attccgcgat tattggcgcg attggtgcac
    15661 tgcgcgggcc gaagcacggc ggggcgaatg aagtgtcgct ggagatccag caacgctacg
    15721 aaacgccgga cgaagccgaa gccgatatcc gcaagcgggt ggaaagcaaa gaagtggtca
    15781 ttggttttgg tcatccggtt tataccatcg ccgacccgcg ccaccaggtg attaaacgtg
    15841 tggcgaagca gctctcgcag gaaggcggct cgctgaagat gtacaacatc gccgatcgcc
    15901 tggaaacggt gatgtgggag agcaaaaaga tgttccccaa tctcgactgg ttctccgctg
    15961 tttcctacaa catgatgggc gttcccaccg agatgttcac accactgttt gttatcgccc
    16021 gcgtcaccgg ctgggcggcg cacattatcg aacaacgtca ggacaacaaa attatccgtc
    16081 cttccgccaa ttatgttgga ccggaagacc gccagtttgt cgcgctggat aagcgccagt
    16141 aaacctctac gaataacaat aaggaaacgt acccaatgtc agctcaaatc aacaacatcc
    16201 gcccggaatt tgatcgtgaa atcgttgata tcgtcgatta cgtcatgaac tacgaaatca
    16261 gctccaaagt agcctacgac accgcacatt actgtctgct cgacacgctc ggctgcggtc
    16321 ttgaagctct cgaataccca gcctgtaaaa aactgctggg gccaattgtc cccggcaccg
    16381 tcgtacctaa cggcgtgcgc gtccccggaa ctcagttcca gctcgacccc gtccaggcgg
    16441 catttaacat cggcgcgatg atccgctggc tcgatttcaa cgatacctgg cttgcggcag
    16501 agtggggcca tccttccgac aatctcggcg gcattctggc aacggcggac tggctttcac
    16561 gcaacgcggt cgccagcggc aaagcgccgt tgaccatgaa acaggtgctg accggaatga
    16621 ttaaagccca tgaaattcag ggctgcatcg cgctggaaaa ctcctttaac cgcgtcggcc
    16681 tcgaccacgt tctgttagtg aaagtggctt ccaccgccgt ggtcgccgaa atgcttggcc
    16741 tgacccgcga ggaaattctc aacgctgttt cgctggcgtg ggtggacggt cagtcgctgc
    16801 gcacctatcg ccatgcgccg aacaccggca cgcgtaaatc ctgggcggcg ggcgatgcca
    16861 cttcccgcgc ggtacgtctg gcactgatgg cgaaaacggg cgaaatgggt tacccgtcag
    16921 ccctgactgc gcctgtgtgg ggcttctacg acgtctcctt taaaggtgaa tcgttccgct
    16981 tccagcgtcc gtacggttct tacgtgatgg aaaatgtgct gttcaaaatc tccttcccgg
    17041 cggagttcca ctcccagacg gcagttgaag cagcgatgac gctctatgaa cagatgcagg
    17101 cagcaggcaa aacggcggca gatatcgaaa aagtgtccat ccgcacccac gaagcctgta
    17161 ttcgcatcat cgacaaaaag gggccgctca ataacccggc agaccgcgac cactgcattc
    17221 agtacatggt ggcgatccca ctgctattcg ggcgcttaac ggcggcagat tacgaggaca
    17281 acgttgcgca agataaacgc attgacgccc tgcgcgagaa gatcaattgc tttgaagatc
    17341 cggtatttac cgctgactac cacgacccgg aaaaacgcgc catcgccaat gccattaccc
    17401 ttgagttcac cgacggcaca cgatttgaag aagtggtggt ggagtacccc attggtcatg
    17461 ctcgccgccg tcaggatggt attccgaaac tggtcgataa attcaaaatc aatctcgcgc
    17521 gccagttccc gactcgccaa cagcagcgca ttctggaggt ttctctcgac agagctcgcc
    17581 tggaacagat gccggtcaat gagtatctcg acctgtacgt catttaagta aacggcggta
    17641 aggcgtaagt tcaacaggag agcattatgt cttttagcga attttatcag cgttcgatta
    17701 acgaaccgga gcagttctgg gccgagcagg cccggcgtat tgactggcag acgcccttta
    17761 cgcaaacgct cgatcacagc aatccgccgt ttgcccgttg gttttgtgaa ggccgaacca
    17821 acttgtgcca caacgctatc gaccgctggc tggagaaaca accagaggcg ctggcgctga
    17881 ttgccgtctc ttcggaaaca gaagaagagc gcacctttac ctttcgccag cttcatgacg
    17941 aagtaaacgc ggtggcgtca atgctgcgct cgctgggcgt gcagcgtggc gatcgggtgc
    18001 tggtgtatat gccgatgatt gccgaagcgc atattactct gctggcctgc gcgcgcattg
    18061 gcgctattca ctcggtggtg tttgggggat ttgcctcgca cagcgtggcg gcgcgaattg
    18121 atgatgctaa accggtgctg attgtctcgg ctgatgccgg agcgcgcggt ggcaaaatca
    18181 ttccctataa aaaattgctc gacgatgcga taagtcaggc gcagcaccag ccacgccatg
    18241 ttttgctggt ggatcgcggg ctggcgaaaa tggcacgcgt cagcgggcgg gatgtcgatt
    18301 tcgcgtcgtt gcgccatcaa cacatcggcg cgcgggtacc ggtggcgtgg ctggaatcca
    18361 acgaaacctc ctgcattctc tacacctccg gcacgaccgg caaacctaaa ggcgtgcagc
    18421 gtgacgttgg cggatatgcg gtggcgctgg cgacctcgat ggacaccatt tttggcggca
    18481 aagcgggcgg tgtgttcttt tgtgcttcgg atatcggctg ggtggtgggg cattcgtata
    18541 tcgtctacgc gccgctgctg gcggggatgg cgactatcgt ttacgaagga ttaccgacct
    18601 ggccggactg cggcgtgtgg tggaaaatcg tcgagaaata tcaggttagc cggatgttct
    18661 cagcgccgac cgccattcgc gtgctgaaaa aattccctac cgctgaaatt cgcaaacacg
    18721 atctctcgtc gctggaagtg ctctatctgg ctggagaacc gctggacgag ccgaccgcca
    18781 gttgggtgag caatacgctg gatgtgccgg tcatcgacaa ctactggcag accgaatccg
    18841 gctggccgat tatggcgatt gctcgcggtc tggacgacag gccgacgcgt ctgggaagcc
    18901 ccggcgtgcc gatgtatggc tataacgtgc agttgcttaa tgaagtcacc ggcgaaccgt
    18961 gtggcgtcaa cgagaaaggg atgctggtgg tggaagggcc gctgccgccg gggtgtattc
    19021 agaccatctg gggcgacgac gaccgctttg tgaagacgta ctggtcgctg ttttcccgcc
    19081 cggtgtacgc cacctttgac tggggcatcc gtgacgctga cggttatcac tttattctcg
    19141 ggcgcactga cgatgtgatt aacgttgccg ggcatcggct ggggacgcgc gagattgaag
    19201 agagtatctc cagtcatccg ggcgttgccg aagtggcggt ggttggggtg aaagatgcgc
    19261 tgaaagggca ggtggcggtg gcgtttgtca ttccgaaaga gagcgacagt ctggaagatc
    19321 gtgatgtggc gcattcgcaa gagaaggcga ttatggcgct ggtggacagc cagattggca
    19381 actttggccg cccggcgcac gtctggtttg tctcgcaatt gccaaaaacg cgatccggaa
    19441 aaatgctgcg ccgcacgatc caggcgattt gcgaaggacg cgatcctggg gatctgacga
    19501 ccattgatga tcctgcgtcg ttgaatcaga tccgccaggc gatggaagag taaggtattg
    19561 tcggatgcgt cgcgtggcgc atccgacagt tgtgcgcaga tgcctgatgc gacgc
//
LOCUS       DAZKFZ010000070        19161 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000070 DAZKFZ010000000
VERSION     DAZKFZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(240..1889)
                     /gene="pgi"
                     /locus_tag="ACHOQW_002334"
     CDS             complement(240..1889)
                     /gene="pgi"
                     /locus_tag="ACHOQW_002334"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9573406.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2126..2322
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2414..3763
                     /gene="lysC"
                     /locus_tag="ACHOQW_002335"
     CDS             2414..3763
                     /gene="lysC"
                     /locus_tag="ACHOQW_002335"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP9573407.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4016..4288
                     /locus_tag="ACHOQW_002336"
     CDS             4016..4288
                     /locus_tag="ACHOQW_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP9573408.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4289..5161)
                     /gene="rluF"
                     /locus_tag="ACHOQW_002337"
     CDS             complement(4289..5161)
                     /gene="rluF"
                     /locus_tag="ACHOQW_002337"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP9573409.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5509..6456
                     /locus_tag="ACHOQW_002338"
     CDS             5509..6456
                     /locus_tag="ACHOQW_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP9573410.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6526..7332
                     /locus_tag="ACHOQW_002339"
     CDS             6526..7332
                     /locus_tag="ACHOQW_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9573411.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7342..7749
                     /locus_tag="ACHOQW_002340"
     CDS             7342..7749
                     /locus_tag="ACHOQW_002340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP9573412.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7749..8243
                     /locus_tag="ACHOQW_002341"
     CDS             7749..8243
                     /locus_tag="ACHOQW_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9573413.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            8309..9106
                     /locus_tag="ACHOQW_002342"
     CDS             8309..9106
                     /locus_tag="ACHOQW_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9573414.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            9117..9941
                     /locus_tag="ACHOQW_002343"
     CDS             9117..9941
                     /locus_tag="ACHOQW_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9573415.1"
                     /translation="MEQRKITLGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9993..11222
                     /gene="sorE"
                     /locus_tag="ACHOQW_002344"
     CDS             9993..11222
                     /gene="sorE"
                     /locus_tag="ACHOQW_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP9573416.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11605..12294
                     /gene="pepE"
                     /locus_tag="ACHOQW_002345"
     CDS             11605..12294
                     /gene="pepE"
                     /locus_tag="ACHOQW_002345"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP9573417.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12385..14016)
                     /locus_tag="ACHOQW_002346"
     CDS             complement(12385..14016)
                     /locus_tag="ACHOQW_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP9573418.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14236..17919)
                     /gene="metH"
                     /locus_tag="ACHOQW_002347"
     CDS             complement(14236..17919)
                     /gene="metH"
                     /locus_tag="ACHOQW_002347"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9573419.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDIANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18119..18943
                     /gene="iclR"
                     /locus_tag="ACHOQW_002348"
     CDS             18119..18943
                     /gene="iclR"
                     /locus_tag="ACHOQW_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP9573420.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4520 a         4988 c         5032 g         4621 t
ORIGIN      
        1 ttccgttaaa tcacagacaa ggggatttat ctgataaaac aatgccgact tatagtcggc
       61 attgttttta aggttattta attaatcgtg ctatgcatta attgccgaat acgatgtaca
      121 tatcggcatc gacctgtagg cctgataaga cgcgtaaagc gtcgcatcag gcaccagttg
      181 ccggatgcgg cgtaaacgcc ttatccggcc tacatagcgg catcaggcat gaacgatgat
      241 taaccgcgcc acgctttata gcggttaatc agaccattgg tcgagctatc gtggctgctg
      301 atttctttat catctttcag ctctggcaga atacggttcg ccagctgttt acccagttcc
      361 acgccccact ggtcgaaggt gaagatgttc aggatcacgc cctgagtaaa gattttgtgc
      421 tcatacagcg caatcaacgc acccaggctg aacggagtga tttcgcgcaa caggatagag
      481 ttggtcgggc ggttaccttc gaacactttg aacggcacca cgtagtcaag cgttgccgga
      541 tctttaccct gatcgcgata ttcctgctca accacttcgc gggatttacc aaacgccagc
      601 gcttcggtct gggcgaagaa gttagacagc agtttctggt ggtgatcaga gagcgggtta
      661 tgggtgatag ccggagcgat gaaatcgcac ggtaccattt tggttccctg gtggatcagc
      721 tggtagaacg cgtgctgacc gttagtgcct ggttcacccc agataatcgg gccagtctgg
      781 taatccacaa cgttaccgtt acggtcaaca tacttaccgt tggactccat attgccctgc
      841 tggaagtacg ccgcgaaacg gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc
      901 gcaccaaaga aattgttgta ccagatgcca atcagcgcca acagtacagg caggtttttc
      961 tcggcaggcg tggtggagaa atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg
     1021 aagttatcaa agccgatgga gagaacaatc gacaggccaa tcgctgacca caaagagtaa
     1081 cgaccgccga cccagtccca gaactcgaac atgttggcag tatcaatacc aaactcgcca
     1141 acggctttgg cattggttga gagcgccgca aagtgtttcg caacgtgttt ttcatcacct
     1201 gccgctttca ggaaccagtc acgcgcgcta tgggcgttgg tcatggtttc ctgagtggtg
     1261 aaggttttag atgctaccag gaacagcgtg gtttccgggt ttactttttt cagcacttca
     1321 gcgatgtgag tcccatcgac gttagaaaca aagtgcatgt tcaggtgatt tttgtacgga
     1381 cgcagtgctt cggtcaccat gtacgggccg aggtcagaac cgccgatccc gatgttcact
     1441 acgtcagtga ttgctttgcc ggtataaccc ttccactcac cggagataat cgcttccgag
     1501 aaggttttca tcttctccag caccgcgttg acttctggca taacatcttt gccatcaacc
     1561 agaatcgggg tattgctacg attacgcagc gctacgtgca gcacggcgcg gttttcagtg
     1621 cggttgatct tctcgccaga gaacatcgac ttaatcgcgc ccgccagatc gcactctttc
     1681 gccagatctt gtaatttcgc cagcgtctct tcagtgatgc ggtttttgga gtaatccacc
     1741 agcatctgat cgtcgaaggt tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa
     1801 agatcggcga tcgtaacgtc tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca
     1861 gcggtctgcg ttggattgat gtttttcatt agcaatactc ttctgatttt gagaattgtg
     1921 actttggaag attgtagcgc cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt
     1981 aatgttgagt gtaaaccctt agcgcagtga agcatttatt agctgaacta ctgaccgcca
     2041 ggagtggatg aaaaatccgc atgaccccgt cgttgacaac cgccccgctc accctttatt
     2101 tataaatgta ctacctgcgc tagcgcaggc cagaagaggc gcgttgccca agtaacggtg
     2161 ttggaggagc cagtcctgtg ataacacctg agggggtgca tcgccgaggt gattgaacgg
     2221 ctggccacgt tcatcatcgg ctacaggggc tgaatcccct gggttgtcac cagaagcgtt
     2281 cgcagtcggg cgtttcgcaa gtggtggagc acttctgggt gaaaatagta gcgaagtatc
     2341 gctctgcgcc cacccgtctt ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg
     2401 acacgaggta gttatgtctg aaattgttgt ctccaaattt ggcggtacca gcgtagctga
     2461 ttttgacgcc atgaaccgca gcgctgatat tgtgctttct gatgccaacg tgcgtttagt
     2521 tgtcctctcg gcttctgctg gtatcactaa tctgctggtc gctttagcgg aaggactgga
     2581 acctggcgag cgattcgaaa aactcgacgc tattcgcaac atccagtttg ccattctgga
     2641 acgtctgcgt tacccgaacg ttatccgtga agagattgaa cgtctgctgg agaacattac
     2701 tgttctggca gaagcggcgg cgctggcaac gtctccggcg ctgacagatg aactggtcag
     2761 ccacggcgag ctgatgtcga ccctgctgtt tgtcgagatc ctgcgcgaac gcgatgttca
     2821 ggcacagtgg tttgatgtac gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga
     2881 gccagatgta gccgcgctgg cggaactggc cgcgctgcag ctgctcccac gtctcaatga
     2941 aggcttagtg atcacccagg gatttatcgg tagcgaaaat aaaggtcgta caacgacgct
     3001 tggccgtgga ggcagcgatt atacggcagc cttgctggcg gaggctttac acgcatctcg
     3061 tgttgatatc tggaccgacg tcccgggcat ctacaccacc gatccacgcg tagtttccgc
     3121 agcaaaacgc attgatgaaa tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc
     3181 aaaagtactg catccggcaa cgttgctacc cgcagtacgc agcgatatcc cagtctttgt
     3241 cggctccagc aaagacccac gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc
     3301 gccgctgttc cgcgcgctgg cgcttcgtcg caatcagact ctgctcactt tgcacagcct
     3361 gaatatgctg cattctcgcg gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa
     3421 tatttcggta gacttaatca ccacgtcaga agtgagcgtg gcattaaccc ttgataccac
     3481 cggttcaacc tccactggcg atacgttgct gacgcaatct ctgctgatgg agctttccgc
     3541 actgtgccgg gtggaggtgg aagaaggtct ggcgctggtc gcgttgattg gcaatgacct
     3601 gtcaaaagcc tgcggcgttg gcaaagaggt attcggcgta ctggaaccgt tcaacattcg
     3661 catgatttgt tacggcgcat ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc
     3721 cgagcaggtg gtgcaaaaac tgcatagtaa tttgtttgag taaatactgt atggcctgga
     3781 agctatattt cgggccgtat tgattttctt gtcactatgc tcatcaataa acgagcctgt
     3841 actctgttaa ccagcgtctt tatcggagaa taattgcctt taattttttt atctgcatct
     3901 ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata ttatcagttc
     3961 tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca ccatcatggc
     4021 actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac ttaaaacact
     4081 gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa ccaacagcat
     4141 taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga aaaaagcccg
     4201 ccagttaaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta gctggtcagc
     4261 aaatacgtcg acgcgcggca ggcgctgatt agcgcccctt cttttttcgc cccggcgagg
     4321 taaaacgctt accgttggac gccgggcgac cgcctttttc cgccgttttt tccatcttaa
     4381 cgactggacg tttgatgccc gctgttttcg gtttggcctt cgctttaggt tttgcctcgg
     4441 aagaggaatt ttcgatgagc ttaaagaggt cgattaactc atcgtcggtt aaatcgcgcc
     4501 actcccccag cggaatgccg cttaagctga cgttcataat gcgcgtacgt tccagctttt
     4561 tcacttcata gccgaaatgc tcgcacatgc gccggatctg acggttcagc ccctgcacca
     4621 gggtaatgcg gaagacaaac ggcgcttctt ttttgacttt gcacttttta gtcaccgtcc
     4681 cgaggatcgg cacccccgcc cccataccac gaataaattc atcggtaatc ggtttatcga
     4741 ccgtcaccag atactctttc tcatgatcgt tacccgcacg gaggatctta ttcaccagat
     4801 cgccgtgatt ggtgaggaaa atcagcccct gggagtcttt atccaggcgg ccaatcggga
     4861 acacgcgttt gctgtggtta acgaaatcga caatgttatc gcgctcgcca tcttcggtgg
     4921 tgctgacaat gcctacgggc ttgttcaggg cgataagtac caaatcttcg gcttcccgag
     4981 gctcaatcaa ctgaccgttt actttcacaa cgtcgccggg tttcacctga tcgccaatgg
     5041 tggctcgctt gccattaagg aacacattgc cttgctcgat atagcgatcc gcttcgcggc
     5101 gtgagcaaat tccgctttcg ctgatgtatt tatttaaacg gactgatgag tcgggcagca
     5161 taaattctcc tgtaaaacgc ggaatatacc gtacctctta gcggggaaaa acagggttat
     5221 gcggcaacgt cgaaattaaa tgtgatctgc cacgcctttt atcacaaaag tgtctccgga
     5281 agtacaatgc aaatttgcgt ggcaaaataa ggataatcaa catattacga atcgctgcac
     5341 acctgtgctg ataactactg cgcagagaaa caaatgtgca agagaaagcg ctatttctcc
     5401 ccatgcaaag agtgaaaaat cgctttgaaa ggccatcatg atggggttat atcagatcaa
     5461 gcggtgagaa agcgtatttg cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt
     5521 gacgatatcc gtttgattgt gaagattgcc caactctatt acgaacagga tatgacgcag
     5581 gcgcaaatcg cgcgcgaact gggtatttac cgcaccaaca tcagccgctt gcttaaacga
     5641 ggccgcgatc agggaattgt caccatcgcc atcaactatg actacaacga aaatctctgg
     5701 ctggagcagc aactgaagca aaagtttggc ctgaaagacg ttgtggtggt gtcgggaaat
     5761 gatgaggatg aagagactca actggcgatg atggggttac acggcgcgca actgctggat
     5821 cgcttgctgg agcctggcga tattgtcggt ttttcctggg gtcgcgcggt gagcgcactg
     5881 gttgaaaact tgccgcaggc ggggcaatcg cggcagttaa tctgcgtacc aattattggc
     5941 ggaccgtccg gtaaactcga aagccgctat cacgtaaaca cattaaccta cagcgcggca
     6001 gcgaagctga aaggggaatc gcatctcgcg gattttccgg ctctactgga taacccatta
     6061 attcgtaatg ggatcatgca gtctcagcac tttaaaacca tctctgccta ctgggataat
     6121 ctggatgtcg ccctggtggg aattggctca ccggccattc gcgacggcgc taactggcat
     6181 gcgttttatg gtggtgaaga gagtgacgac ctgaatgccc gccaggttgc tggcgatatt
     6241 tgctcgcgct tttttgatat tcacggcgaa atggttgaaa cgaatatgag cgaaaaaaca
     6301 ctctctatcg aaatgaataa attaaagcag gcacgatatt ccattggcat tgccatgagt
     6361 gaagaaaaat acagcggaat tattggtgca ctgcgtggaa aatatattaa ttgtctggta
     6421 acgaatagca gcacagctga actattactg aaataaaaat cttcccgatg tttatcgtaa
     6481 taaaaattac ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta
     6541 aatttgcagg ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt
     6601 gtagaggaat tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat
     6661 ggacaatatg aaggtcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa
     6721 gaggtaaatc atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc
     6781 aataacgccg gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag
     6841 tatgaactca acgaagctgc attcgaaaaa atggtcaata tcaaccagaa aggcgttttt
     6901 ctgatgtcgc aggcggtggc gcgacagatg gtcaaacaac atgatggcgt gattgtgaat
     6961 gtttcctcag aaagtgggct ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa
     7021 gccgcgctca atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt
     7081 gtggtcggta tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa
     7141 gaggcgctgg cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa
     7201 aacgccattc ctattgggcg cgccggaaga ttagcagaaa tcgctgattt tgtttgttat
     7261 ctgctgtctg aacgcgccag ctatatcacc ggagtaacca ctaacattgc gggcggcaaa
     7321 acgcgcggct aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct
     7381 tgcgccatgc tggaatcggt tcagatggtt tacggcaacg ccaatgttga agccgttgaa
     7441 tttgtgcccg gtgaaaacgc cggagacatc gtagcaaagc tggaaaaatt agtaagcatt
     7501 cacaatcaaa atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg
     7561 gcagcgatgc ttgcgatgcg caatcctcgt ctgcgggtaa tcagcggact ttctctgccg
     7621 ctggcgctcg aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac
     7681 ctgacgcaaa tcgcaaagca aacctgcgtt gtctggaaac aactggcaac gacagaggag
     7741 gatttctgat gaacattacg ctcgcccgca ttgatgaccg cctgatccac ggtcaggtca
     7801 ctaccgtctg gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt
     7861 ataacgatga agttcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta
     7921 acgtggtcaa tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat caggacgaaa
     7981 ccgtttttta tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa
     8041 aaataggcac cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca
     8101 aagcggtttc attagatgat gatgatatta acgcatttca tgagttaaat aatctaggcg
     8161 tcatactgga tttacgcgtc gtagcgtcag atccctcaat caatattatc tacaaaataa
     8221 acgaacagtt aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg
     8281 gatgacgaca cttaaaaggt gacaaatcat ggaaataagt accctacaaa tcattgctat
     8341 atttcttttt tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg
     8401 cccgttaatt gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat
     8461 actcggtggt acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc
     8521 tccggattct gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag
     8581 catcgccacc ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccagg tgctgacggt
     8641 gtttgcccgt accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaagc
     8701 acggtttcgc actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt
     8761 cgccattccg gcactgattg tctcgctgtt cgtcagcgcc gatatggtga gcaatatgtt
     8821 aaacgccatt cccgaatttg tgacccgagg tctgcaaatt gctggcggtt ttatcgtggt
     8881 agtcggttac gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt
     8941 cctcggtttc ctcgcaggtg gctacctcga tctcagtctg ctggcgttcg gtggcgtcgg
     9001 cgtgatcatg gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca
     9061 actcctggcc cccgcttcca ccgcccttga ccaacttgac gattaacgga gccatcatgg
     9121 aacagagaaa aattacactc ggcgatctgg tgagcatgtt tctgcgctcc aacctgcaac
     9181 aggcgtcctt taactttgaa cgtattcacg ggctgggttt ttgctacgac atgatccccg
     9241 ccatcaagcg actttaccca gtaaaagagg atcaggttgc ggcgctcagg cgacacctgg
     9301 tgttctttaa taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg
     9361 aagaggcgcg ggccaacggc gcggaaattg atgacggcac cattaacggc atcaaagtcg
     9421 gtctgatggg accattggca ggggttggcg atccactggt ctggggaacg ctgcgcccga
     9481 ttaccgccgc gctcggcgca tcgctggcgc tgtctggcaa cattctcggc ccgctgctgt
     9541 tcttctttat tttcaacgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct
     9601 ttcgcaaagg ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg
     9661 aaggcgcgtc gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa
     9721 tcaacgtacc gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcaccatga
     9781 ccgtgcagaa cattctcgat caactttgcc ctggtttgct ggcgctcggt ctgacgctac
     9841 tgatggttcg tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg
     9901 gcttagggat tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac
     9961 tgccggggcc atcgttcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg
    10021 gtaaacgtga tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc
    10081 tcgcgacggt ggtcactgac agcctgtgcc tctcttcctg gaaagaggcc aatctgggtg
    10141 aaaaccataa aaaagtaccc gacgatgtgg cgaccaaccc aatcatcatc ggccacgagt
    10201 tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag ccgggtcagc
    10261 gttatgtgat tcaggccaac ctgcaactcc ccgaccgccc ggactgcccc ggctactcct
    10321 tcccgtgggt aggcggcgag gccacgcatg tggttattcc caacgaggtc atggaacaag
    10381 attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcgctggtt gaaccgcttt
    10441 cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt tataaccaca
    10501 cgatggggat tcgcccgcaa gggcgcatgc tgatcctcgg cggcaccgga ccaatgggac
    10561 tgttggcgat tgattatgcg ctacatggac ccgttaaccc gtcgctgctc gtcattaccg
    10621 ataccgacaa cgataaattg agttatgcgc gcaagcacta tccatcagaa ccgcaaacac
    10681 tgattcatta tctcaatgcc gccgatgcag catttgatac gctaatggcg ctgagtggcg
    10741 gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg actctcgcct
    10801 cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag gataaacatt
    10861 tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac gtgggcacgt
    10921 caggcggcaa taccgacgac atgcgcgcag cggtcaaatt gattgaagag aaaaaagtgc
    10981 aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa accacgcttg
    11041 aattgcctgc cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac ctgccgctga
    11101 cgtcactcac gcagattcag gatcaagcac tggcggcgat tctggcgcgt catcagggga
    11161 tctggtccgg tgaggcggag caatatctgc tcactcatgc agaggcaatt tcccatgatt
    11221 aatcgcgcta cttaactctg catgtcgctg gctggtcacc gcggcaactt cgcgacctga
    11281 tgtgcggctg attgctgaag cggcccaatg tgcacgagaa ggataaaaaa acatcagcgc
    11341 cttaaggggc gctcaaattg gtggcaaagt acacattgtt tatgccggat gcggaataaa
    11401 gccttattcg gcctacataa tctcgaaaat tcaatatatt gcagttatct ggtaggcctg
    11461 gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata
    11521 cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca tgccttccgt
    11581 gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc gacgctgccg
    11641 ggtaaagcct ggctggaaca tgcactgccg ctgattgctg aacagttgca gggtcgccgc
    11701 tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta cacagcgaaa
    11761 acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat
    11821 cccgttgccg cgattgaaaa tgcggaaatt gtgattgtcg gcggcggtaa tactttccag
    11881 ttgctgaaac agtgccgcga gcgcgggctg ctggctccaa ttactgacgt ggttaaacgt
    11941 ggcgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc
    12001 accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgttg
    12061 caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag
    12121 cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa
    12181 ggtaactgga tcacagtgag taaaggtcac gcaacgctgg gtggcccgaa taccacctat
    12241 gtgtttaagg ccggtgaaga agcggttccg ctggaagctg gtcaccgttt ttaagtgaat
    12301 tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt
    12361 atcaggccta caggattcac aatattaata ctcgtcccgt ccctcatctt catcaggctg
    12421 ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag
    12481 taagcctaaa tgtagcgaac tggtttcaat gctttgcacg ttttgctgat gcaggcgatc
    12541 gacgtgggcg tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa
    12601 acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact
    12661 gagcaattgc tcatagagcg catccagttc cttcaagcca tcaagcgaaa atgctcgcct
    12721 tgcagccagc gatttgtcgg caatttcact actcatgcgc tcgacgatat cggaggcctg
    12781 ttcaaggttg agcgacattt cgatgatctc cgcccagcgg cgcgactctt cttctgccag
    12841 ttcctctttt ggcatccgcg ccagatacag cttaatggcg gtatagagaa cgttgatatc
    12901 atctgccagc ttacgcagct ctttctcctg ccttggctcg ccgtgcatca ctttattcag
    12961 cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc
    13021 cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt
    13081 atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa
    13141 tggcagcatg gccaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc
    13201 cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat
    13261 aatcaggcta cccaccagct taaacagcag actacccagc gcgacacggc gggctgcggc
    13321 attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac
    13381 cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag
    13441 tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag
    13501 cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg
    13561 cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac
    13621 gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa
    13681 aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt
    13741 cccgacatct gcccccagca caatgaccag agccggtgcg agggctacca gatcctgagc
    13801 gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc
    13861 gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa
    13921 acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag
    13981 ggcgacggca gaaagcaggt taagcagcgt taacacgagt tcaggttctc cttatcgtta
    14041 tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac
    14101 tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt
    14161 gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag
    14221 tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac
    14281 atcggagacg ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg
    14341 cgccaccgcg tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc
    14401 gggccacatg gcgaaagatt ctgtgagttt catgccggta tgtttttcca cttccagcag
    14461 ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg
    14521 gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata
    14581 gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg
    14641 gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc
    14701 agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt
    14761 accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc
    14821 ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta
    14881 gatttcaatg tcatcgccca cacggtttgc cgggaacagg cccaccacgc cacgcggatt
    14941 cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg
    15001 cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca
    15061 ggtcataaag aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac
    15121 ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa
    15181 gtcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgtggtttct tgcgcccgtg
    15241 ctgaatacgt acggtttcgt actccttgcg ggtacgagcg acaaaatcat cgcgctgagt
    15301 atcggaaagc agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt
    15361 cgggccgctg tagttctgct cgattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc
    15421 accgatcagt aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat
    15481 ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt
    15541 agcggtacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt
    15601 gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac
    15661 ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg
    15721 ttcgaggtag gccactgcct gcttcatcac acgcgccgat ttgaccacct gtggcaggaa
    15781 cattttccct tcgccgaaca gatcgccgac cacgttcatg ccgtccatta gcgggccttc
    15841 aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa
    15901 ctcagtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca
    15961 ctccgcctgc tgggcgttag cgatgtcgtc ggttttgctg ccgcgatatt tctcggcaag
    16021 ctccagtaaa cgctcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc
    16081 gcgcagttca gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat
    16141 atccatgcca ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg
    16201 atcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg
    16261 cagttcgcgt ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc
    16321 ttcaatgccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa
    16381 gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcaattt tccgtgcgcg
    16441 ggtatcggcc tgcccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg
    16501 caacagtttc gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac
    16561 aatgcctttg ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc
    16621 gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac
    16681 catcgccgct tcggcatcga gcatcccttc atccatgttg atatcgataa tctgcgcgcc
    16741 gttttccacc tgctgacgcg ctacatccag cgcctcgctg tatttctctt ctttgatcag
    16801 gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct
    16861 atcgtcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg gaatttcagg
    16921 cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt
    16981 ggtgccacag cagccgccga cgatattgag aaaacccgct tgcgcccatt cacgtatctg
    17041 ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt
    17101 cgggtgcgcg gtgacgtagc attccgcaat ccgtgatagt tcctgcacgt actggcgcag
    17161 ttcatctggc cccagcgcgc agttgaggcc aaaagtcagg gcttcggcat ggcgcaggga
    17221 gttgtaaaat gcttcggtgg tctgtccgga gagcgtgcgc ccggaggcgt cggtgatggt
    17281 gccggagatc ataatcggca gctcaacgcc taacgcttcg aactccgttt tcaccgcaaa
    17341 gaccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcagaatca gatccgcacc
    17401 gccttccact aatgctttgg tggattcgcg ataggccgcc accagctggt caaaagtgat
    17461 gttgcggaat gccggatcgt tcacgtccgg agaaatggac gccgtgcggt tggttgggcc
    17521 gagaacgccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca
    17581 ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta
    17641 atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc
    17701 ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttgcttagca ccagcagatc
    17761 gttattgcct ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc
    17821 gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc
    17881 atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa
    17941 cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg
    18001 accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt
    18061 gcattagcta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat
    18121 ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac
    18181 tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa
    18241 tggcagtgtg gcactcacgg agctggcgca acaagccggg ttacccaatt ccacgaccca
    18301 ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca
    18361 ctgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt
    18421 gttagcaatt gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa
    18481 tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgaccagg tacagtgtac
    18541 gcatctgatg cgaatgtccg cgcctatcgg cggtaaattg ccgatgcacg cttccggtgc
    18601 gggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa
    18661 agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct
    18721 cgcccaaacg cgcaaacggg gttattcatt tgacgatgag gaacatgcgc tggggctacg
    18781 ttgccttgca gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc
    18841 cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa
    18901 agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag
    18961 aggcaatatt ctgcccgtca tacctgagtg gtaatagaat aagggtgtct gttaatcgca
    19021 ttgacgtcaa aataacttat tgtcatacac ttcatacact tttattaact cagcattatt
    19081 tttaaacatc aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt
    19141 ttacggctgc attatgagat t
//
LOCUS       DAZKFZ010000071        19059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000071 DAZKFZ010000000
VERSION     DAZKFZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(114..479)
                     /gene="yhaH"
                     /locus_tag="ACHOQW_002349"
     CDS             complement(114..479)
                     /gene="yhaH"
                     /locus_tag="ACHOQW_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9573421.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(771..1757)
                     /gene="yqjG"
                     /locus_tag="ACHOQW_002350"
     CDS             complement(771..1757)
                     /gene="yqjG"
                     /locus_tag="ACHOQW_002350"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP9573422.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(1827..2219)
                     /gene="yqjF"
                     /locus_tag="ACHOQW_002351"
     CDS             complement(1827..2219)
                     /gene="yqjF"
                     /locus_tag="ACHOQW_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9573423.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2405..2704)
                     /gene="yqjK"
                     /locus_tag="ACHOQW_002352"
     CDS             complement(2405..2704)
                     /gene="yqjK"
                     /locus_tag="ACHOQW_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP9573424.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2694..3098)
                     /gene="yqjE"
                     /locus_tag="ACHOQW_002353"
     CDS             complement(2694..3098)
                     /gene="yqjE"
                     /locus_tag="ACHOQW_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9573425.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3101..3406)
                     /gene="yqjD"
                     /locus_tag="ACHOQW_002354"
     CDS             complement(3101..3406)
                     /gene="yqjD"
                     /locus_tag="ACHOQW_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP9573426.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3444..3812)
                     /gene="yqjC"
                     /locus_tag="ACHOQW_002355"
     CDS             complement(3444..3812)
                     /gene="yqjC"
                     /locus_tag="ACHOQW_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP9573427.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3959..4342)
                     /gene="mzrA"
                     /locus_tag="ACHOQW_002356"
     CDS             complement(3959..4342)
                     /gene="mzrA"
                     /locus_tag="ACHOQW_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP9573428.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4346..5008)
                     /gene="yqjA"
                     /locus_tag="ACHOQW_002357"
     CDS             complement(4346..5008)
                     /gene="yqjA"
                     /locus_tag="ACHOQW_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP9573429.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5030..5113)
                     /gene="yqiM"
                     /locus_tag="ACHOQW_002358"
     CDS             complement(5030..5113)
                     /gene="yqiM"
                     /locus_tag="ACHOQW_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP9573430.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5353..6129)
                     /gene="exuR"
                     /locus_tag="ACHOQW_002359"
     CDS             complement(5353..6129)
                     /gene="exuR"
                     /locus_tag="ACHOQW_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP9573431.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6259..7557)
                     /gene="exuT"
                     /locus_tag="ACHOQW_002360"
     CDS             complement(6259..7557)
                     /gene="exuT"
                     /locus_tag="ACHOQW_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP9573432.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            8040..9452
                     /gene="uxaC"
                     /locus_tag="ACHOQW_002361"
     CDS             8040..9452
                     /gene="uxaC"
                     /locus_tag="ACHOQW_002361"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9573433.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9467..10954
                     /gene="uxaA"
                     /locus_tag="ACHOQW_002362"
     CDS             9467..10954
                     /gene="uxaA"
                     /locus_tag="ACHOQW_002362"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP9573434.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11037..11588
                     /gene="ygjV"
                     /locus_tag="ACHOQW_002363"
     CDS             11037..11588
                     /gene="ygjV"
                     /locus_tag="ACHOQW_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP9573435.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11593..12837)
                     /gene="sstT"
                     /locus_tag="ACHOQW_002364"
     CDS             complement(11593..12837)
                     /gene="sstT"
                     /locus_tag="ACHOQW_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9573436.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13236..14201)
                     /gene="alx"
                     /locus_tag="ACHOQW_002365"
     CDS             complement(13236..14201)
                     /gene="alx"
                     /locus_tag="ACHOQW_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP9573437.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(14485..15471)
                     /gene="ygjR"
                     /locus_tag="ACHOQW_002366"
     CDS             complement(14485..15471)
                     /gene="ygjR"
                     /locus_tag="ACHOQW_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9573438.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15550..16242)
                     /gene="ygjQ"
                     /locus_tag="ACHOQW_002367"
     CDS             complement(15550..16242)
                     /gene="ygjQ"
                     /locus_tag="ACHOQW_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP9573439.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(16319..16822)
                     /gene="ygjP"
                     /locus_tag="ACHOQW_002368"
     CDS             complement(16319..16822)
                     /gene="ygjP"
                     /locus_tag="ACHOQW_002368"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9573440.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16907..18043
                     /gene="rlmG"
                     /locus_tag="ACHOQW_002369"
     CDS             16907..18043
                     /gene="rlmG"
                     /locus_tag="ACHOQW_002369"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP9573441.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18327..18641
                     /gene="higB"
                     /locus_tag="ACHOQW_002370"
     CDS             18327..18641
                     /gene="higB"
                     /locus_tag="ACHOQW_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFP9573442.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18638..19054
                     /gene="higA"
                     /locus_tag="ACHOQW_002371"
     CDS             18638..19054
                     /gene="higA"
                     /locus_tag="ACHOQW_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP9573443.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         4891 a         4922 c         4822 g         4424 t
ORIGIN      
        1 aggtaattta tataacgaca ttgaaatatt ttaaataaac attagtaaat tattcattca
       61 tcaacatgat gataaaagcc ggatgagtca gacatccggc gagcagattt gttttaaggt
      121 tccagttttg gatcaggtcc aaagcggttt tcgcctggcg tacccgcctg gcagttaaag
      181 acgatgataa taagccagcc gataaacggg attaagaata gcagtgccca ccacgccgag
      241 cggtcggtgt cgtgcaggcg gcggaactga actgcccacc acggtaaaaa caccagaata
      301 ccgtagattg tcgtcaggat gccttcgcca ccagcgcgtt gccagcctaa cattttatcc
      361 agcagcccca gcacgaacgt aaagatgatg ttgaccagaa taaacatcca gtactctttg
      421 cgccgcgcac gaccccggaa accgacataa ttttttagta ctttcagata ccagtccatt
      481 tttgcctcaa ttttgctcaa tcacttgttt ttaaagcagc aaattaagaa tagacgtgaa
      541 tgaaatggca gtaaatattc gttcaaggaa ttaataaaat gctgtgaagg cgcgctaaga
      601 ctgatgacaa gcgcaaaatt gccagatgcg ctacgcttat ctggcccgcg cggtcattgc
      661 aacatattga atataaaagt tttgtaggtt tgataaggcg ttcatgccgc atccgacatg
      721 aacaacatgc acttagctaa ttatcagagg cacctcgtaa ggtgcttttt ttaacctaag
      781 cgaacatctc gtccatgcgg ttcatcgaga tcctgccacg gaccaattga aataatccgc
      841 gtagggttga tggtcttatg actgcggaag taatgattac ggatatgatc gaaattgact
      901 gtttcggcaa ttcccggcat ctggtagata tcacgcagga agccatacag attaaggtaa
      961 tcgctgatgc ggtgcttatc acacttgaag tgggtcacat acactggatc aaaacggacc
     1021 agcgtggtcc acaggcgaat atcggcttcg gttagctggt tgccggtcag gtaacgatgc
     1081 tgacctaaaa tctgttccag tcgcgccagc gattcaaaca ctttctccac cgcttcgtcg
     1141 taggcttgct ggctggtggc aaaaccagct ttatacacgc cgttgttaac ggtgtcataa
     1201 atccagccgt taagttcgtc aattttcgtt tgcagggctg gtgggtagta atcacccgct
     1261 ttcgcgccca gcgcatcaaa cgcggtatta aacatgcgga tgatttctgc tgattcgttg
     1321 ctgacgatgg tgtggttctt tttgtcccac agcacgggaa cagtaactcg tccgctgtag
     1381 tgtggatcgg cgtggagata aagctgatac aaaaattcat gttgatagag cgtgtctccg
     1441 gttgctcccg gaaaactgtc atcaaaggtc cagccgtttt ccagcatcag cgggttcact
     1501 acggaaacgg aaataaacgg ttccagtcct ttgagtttgc gcatgatcag cgtgcggtgc
     1561 gcccacgggc aggcgagtga aacatagaga tgataacgat ctttctctgc gataaaaccg
     1621 cctgtgccag tggggccagg agcgccatcg gcagtgagcc agttacgaaa tgcggaagct
     1681 gaacgttgaa atttaccgcc ggtagatttg gtatcgtacc aggtgtcatg ccagacgccg
     1741 tcaatcagtt gacccattgt tctctcctcc aatagcaaaa acgaggagct tcctcctcgt
     1801 tttttatcgt taagtatagc gcgtgcttac cactttttat tcagcaggcg gtcgatgcta
     1861 tacgcgcccg gaccggtaat tgccagcagc aggaatccgc cagaaattgt caggtttttc
     1921 atgaacatca gcgagttgac gccttcagca aagttgctgt gaaataaaaa tgccgtcagc
     1981 agcgtaaagc ccgcagtaaa cagggctgtg gtgcgagtca ggaaaccgaa caggattgcc
     2041 agaccaccac caaactcaag cagaatcacc agcggcagca taaaacccgg gacgcccatc
     2101 gcttccatat attgttgggt acccgcgtaa ccagtaattt ttccccagcc tgcggtaata
     2161 aacagaatcg gcattaaaat gcgcgctacc agtacaccaa catcttctaa ttttttcatc
     2221 atactctcca ctgcgccact caggctgctg attgtttttt tgtgcaaggg cgcggtatta
     2281 gcgtcgttgc tgtcgatgga gagaatcata aacgtggtga atgatgattg ttagcaagga
     2341 aaactgtcaa aaatcttcaa aaaatttgat gggtaaggcc ggaatggctc cggccagagg
     2401 gaagttaacc gcgaagctgt tgctgcttga gggtcgtttt aaccagacgc caggcgctcc
     2461 atacgccaaa accgcgtctg gcccagcgga ccagcatatt aggatggcga atcgtccaga
     2521 tcgccatcac gctactgcca accagcgccc aggagcgcag acttagcagc atattccagc
     2581 gacgatcgta agcgcctgtt gcctccagcc attcacgacg actggcagaa agatccagcc
     2641 gttgttgctg gatctggcta agcagttgcg ccttacgtcg ttcacgttcg actttactgc
     2701 tcacgggact cctcctcgag cagctgccga tcgtttgcca gctcatggcg tgtatggcgc
     2761 agcaaggtgg atttacgcga tttacgtaac gtccagatac cgccaatcag tgccagtagc
     2821 agcaacacca cggtggtggc aatcatcgca ttcaggcgat attgcggatc aaccgcccaa
     2881 ataattagca ccatcaggct cataagacca aatgcagcga aaagcatcgt caggcccagc
     2941 atcagtaaaa gttgaaagag attagctttt tcctcttcca gctcaaccac cgccagccgc
     3001 agacgtgttt ccaccatttc aaccatgatg gaaacaattc gctgcccgat gcccagaacg
     3061 cttttaccgg gcccttgcgc gtgatgagtg tccgccataa ttaacgacgc gacagcagaa
     3121 cgccgagcac tacaccgatt gcagcgccaa tgcccacgcc cgtccatgga ttttcacgca
     3181 catactcatc ggcacgcgcc gccgcgacac gggtttgttt ggcgatggca tcaccggttt
     3241 cacccaggcg ataacggctc tgtttcagtg cctgctccgc tttgctacga atcttactca
     3301 actcttcttt cgacttctcg ccagatgagc taagcacctc ttccagcgta tcggaaaggg
     3361 atttcaactc agcacgcaga tgttccgtag tgtgttcttt cgacatagtt ttctccaggt
     3421 gagtaagtag tgactattgt gagttagtag tcgcgcgctt ccagcttttt cagctcttcc
     3481 tgcgcttctg ccagtttccg ttcgcgtttg gcaatcttat cggcatcgcc tttttgcttc
     3541 gcctcggcta aatcttgctg gcgttccgcc acctcatctt tctgctttgc gattttcttc
     3601 tgatgatcgg cacgcagctg gctatctgaa cagttggccc ggacttcact cagggcttta
     3661 ttcagaccgt caatacgatt ctggttttgg tgtttttcgg catagctgat ctccttaagg
     3721 atattttgct ccttttcctg acacagggta gtggcataac taccggcact aagagcaaaa
     3781 agagaaacag ctaaagcgat gcggtatttc attcttgaac cttccattgt gattcggacc
     3841 tattacaaag gaacgattgt ccagacaacg aagcggtagt gttaaccacc gcctcattaa
     3901 tcatagacag ttacggtgaa atcaccaaag tgagtgatct gtttcggaca acaggaagtt
     3961 atccgaagcg atgagagtta tcccgtagcc gggtcagcca ctgcatagcc tgactgttat
     4021 tgtcctgctg cgcaatgatg tagccttggg gcaatgttct gtcgaggacc gcttttgcag
     4081 cggcgctctg gtcgctggag tcaaaggtaa ttaacagagt gtcgtttttg ggggtgatac
     4141 ttttgaaagg aatgccatga gcgtcaaggt gatgccagat tgaaaaaccg tctggcatcg
     4201 ttgtgccttg atgaacggca cgaatcgcca gcgtagactc ttgctggcga accgcggacc
     4261 aggccagcaa cagcgtgccg accagaagta aaacagcgcc ggaccaggct agctggcgaa
     4321 gcgacatgcg aggtatttgc atcccttacc cccgatttcc atatttcttt ttccataata
     4381 cgaccagaga acctgccagg ccaaacacca gcagcaccac cgggagcagc atcaggcatg
     4441 acatcagctg gtcctcgtac tttaaaaata ccggcgtttt gccgagcatg taacccagag
     4501 ttgtcaggat caatacccac agcagaccgc tcatccagtt gaaaaactga aagcgcgcgt
     4561 tattcagccc tgataacccg gcaatcgtcg gcagcagtgt tctgacaaac gcaataaagc
     4621 gaccaattaa cagcgccgat aaaccgtgtt tatgaaaaag atggtgtgcg cgttgatgat
     4681 aatgcgcggg taaatgagat agccagtttt gtacggtgcg ggtattgccc agccatcgcc
     4741 cctgaatata gctgacccag cagccgaggc tggcggcaac ggtcagcagc agaatcgttt
     4801 gcggatagcc catcgcgcct ttcgcaatca acacgccgac caataccagt aaactgtcgc
     4861 ccggtaaaaa ggccgccgga agcaagccgt tttcaaggaa caaaattaca aacaagacaa
     4921 aatacaacat gccaatcatc gatggattgg ccagggtttc aaaatcctgc gcccacaggg
     4981 cttgcagcaa ttgggtcaaa agttccattc attattcctg gtgaatcggt taacgccacg
     5041 ctggttttaa aagatgcact accgcaatgt tattgctgtt ttggtatgtt ctgcgggttt
     5101 gatacatcaa cacagcctga actattccct gttaaatcgt gagataagaa agcattcaaa
     5161 aacataaatt caaattgaat ctaattgtaa caaaggtcaa cgttgttcgt tatcaaaatt
     5221 acatgtccgt ggagtctgat tttgctgaat gcaggggagg gtggcgagag gatttacaca
     5281 ggctgacact ttatactttt cgcttaccga cgagacaaac aggcgcgtcg cagcgcctgt
     5341 cggacaggta tttcatttac tgccactggt ggccgtatcg agatgcacta ccgggttttc
     5401 ggcgaacaga tagcggtcgg cattgaactc gaagtcgtcg ctggtttcgt tgaataacat
     5461 gatcttggtg ttttccaggt gctgccacat tgccagctta gcagcatgag gatctttgcg
     5521 aatcagcgcc ttgaggattt gatcgtggtc atcacaccag ttatcgacgg tacgggaatc
     5581 aatgtgttcg tgcagttttt tccagtacgg gttatggcta cgctgggtcc acattttttc
     5641 aacgatagcc gccagggcgg agttctgcgt cgccagggct acctgaatat ggaactgcaa
     5701 atcccactcg gaatcacgga agcattgttc gccgcgcgcc tgctcctgga tggccatcaa
     5761 tttcatgatg tcctgtttcg ttacctgagt tgctgcgaac tcggcaatat tactttcgat
     5821 gagttggcga gcctggagta actcaaacgg accgtaattg gcgaattcca tattattgtc
     5881 cgcagcctgc tgatggcgcg gctggttgga aaccacgtga atacctgaac ctttacgcac
     5941 ttcgacatag ccttcaactt ccagcatgat gatggcttca cgaactaccg tacggctgac
     6001 gttcttttca tcggcaataa agcgctcagc aggcagttta tcacccacca gatagacgcc
     6061 ctgttcgatg cgctctttca ggtcagcggc aagttgttga tacaaacggc gtggttcagt
     6121 gatttccata tgcgctccag gcatgatacg gcagactata tttgttatac cacttttgcg
     6181 agtttagctc tactcgcagt gatgaaaaag ccgcctgaga aggcggcttt gaagcgaaac
     6241 taaaacttac acgaagcgtt aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac
     6301 ctcaatcgcc ggtttgttct gcaatacggt ccagataacc agcgcaccca gcaggtcaaa
     6361 tactgccaga actgcgaaca gcgggctgaa gccgatggtg tcagccagtg caccgacaac
     6421 cagcgcaaac agcgtacttg ccagccatgc ggacatcccg gttaaaccgt tcgccgttgc
     6481 cacttcgtta cgaccaaaca cgtcggaaga gagcgtaatc agcgcgccag acagtgcctg
     6541 gtgggcaaaa ccaccgatac acagcagcat aattgcgaca tacgggttgg tgaacaggcc
     6601 gatcataccc gggccaatca tcagcactgc acccagcgtt acgaccatct tacgggaaac
     6661 gatcaggttc acaccaaacc aacgctggaa cagcggcggc aggtaaccac cgaggataca
     6721 accgaggtca gcaaacagca tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa
     6781 gccgtaaact ttaaacatga acagcgggat ccacgcgtta aaagtacccc aggccggttc
     6841 tgccaggaaa cgcggcagcg cgatacccca gaactgacgg ttacgcagga tctgaccaac
     6901 ggacattttc ttcgccgtac tcacctggtg ctgggcttcc tgaccattaa taatatagtc
     6961 gcgttcttca tcggtcagat gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc
     7021 catcgcccag ataaagctca atgcaccgga gatgataaat gccatctgcc agctgtgcat
     7081 cacgattgcc cataccacca gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt
     7141 aaaatagcct actgcgatgg aacgctcttt cgccgggaac cattcggagc tggctttcag
     7201 acccgccggg atcatcgctg cttccgcggc accgaccgca ccacgagcaa cagccaggcc
     7261 accccagcta cctgccagcg cggttgcacc acagaacacg gcccacagca cagcaaacat
     7321 tgcataaccg attttcgtac ccagcacatc cagtacataa cctgctaccg gttgcatgac
     7381 cgtataagca gcagaatagg ctgcgatgat ataggaatac tgttgggtgg agatgtttaa
     7441 ctcttccatc agagttggcg cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac
     7501 ggtgccgagc gtcaccagtg cgatcatata ccaacgtaac cctttaattt tacgcatgta
     7561 aaacctcatc gttttgttat atccgcagga agcgcggcca tcatcccgcc agaggctgaa
     7621 gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa
     7681 agtttcgtgc taattcggct tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa
     7741 tcggcaattt gcattccgtc gacttatgat ttgcgacggc agaaagataa cttgtcatac
     7801 aactttaaaa ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca ttacctgacg
     7861 acagcgaggc cagtactggc gcggcctgca gagagattta ccactttgag agtaattttt
     7921 ttaactacgt ttattgatct aactcacgaa aatatcttcg gactctggaa attggtgtga
     7981 taactttgtc agcatcgcac cataagcaag ctagctcact cgttgagagg aagacgaaaa
     8041 tgactccgtt tatgactgaa gatttcctgt tagataccga atttgcccgc cgtctgtatc
     8101 acgactacgc aaaagaccag ccgattttcg attaccattg ccatttgccg ccgcagcaga
     8161 ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg gctgaaaggc gatcactaca
     8221 aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct gtgtaccggt gatgcgtctg
     8281 accgtgaaaa atttgacgcc tgggcggcga ctgttccgca cactatcggc aacccgttat
     8341 accactggac gcacctcgaa ctgcgtcgtc cgtttggtat cactggcaaa ttgctttctc
     8401 cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact gctggcgcag gataacttct
     8461 ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt cggcaccacc gatgacccga
     8521 tcgattctct ggagcatcac gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc
     8581 tgccgagctg gcgtccggac aaagccttca acatcgaaca ggcgaccttt aacgactaca
     8641 tggcgaagct gggcgaagtt tccgataccg acattcgccg ctttgctgac ctgcaaactg
     8701 ccttgaccaa acgtctggat cacttcgccg ctcacggctg taaagtgtct gaccatgcgc
     8761 tggacgtggt gatgtttgct gaagcgaacg aagcggaatt ggacagcatt ctggcgcgcc
     8821 gtctggctgg cgaaaccctg agcgagcacg aagtggcaca gttcaaaact gcggtactgg
     8881 tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc
     8941 tgcgtaataa caacctgcgt cagttcaaac tgctggggcc ggatgtaggc tttgactcca
     9001 tcaacgaccg tccgatggca gaagagttgt ctaagctgct gagcaagcag aacgaagaaa
     9061 acctgctgcc gaaaaccatt ctgtactgcc tgaacccgcg cgataacgaa gtgctgggca
     9121 ccatgagcgg taacttccag ggcgaaggta tgccgggcaa aatgcagttc ggttccggct
     9181 ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat gacccaactg gcgcagctcg
     9241 gtttgctgag ccgctttgtc ggtatgctga ctgacagccg tagcttcctg tcatacaccc
     9301 gtcacgaata cttccgccgc attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg
     9361 aagcaccggc ggacatcaac ctgctgggcg agatggtgaa aaatatttgc tttaacaatg
     9421 cgcgtgacta cttcgccatt gaactgaact aaggtctggg gttgatatgc aatacatcaa
     9481 gatccatgcg ctggataacg tcgcggtcgc tttagcggat ttggctgaag gcacagaagt
     9541 cagtgtcgat aaccaaactg ttaggctgcg ccaggatgtt gctcgtggac ataaatttgc
     9601 gttaacgaat atcgcaaaag gggccaacgt catcaaatat ggcctgccga ttggttatgc
     9661 attggcggat attgcggcgg gggaacacgt tcacgcccac aatacgcgca cgaatctgag
     9721 cgatctggat cagtatcgct atcaacctga ttttcaggat ctgcctgcgc aagcggcaga
     9781 tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg
     9841 gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg cagatccaga accgttttct
     9901 gaaagagacc aacaacgctg aaggtaccga cggcgtgttc ctcttcagcc acacctacgg
     9961 ctgctcacag ctgggcgacg atcacattaa tacccgcacc atgctgcaaa acatggtgcg
    10021 ccacccgaac gcaggcgcag tgctggtgat tggtctgggc tgtgaaaaca accaggttgc
    10081 cgcattccgt gaaacgctgg gcgatatcga tcctgaacgc gttcatttca tgatctgcca
    10141 acagcaggat gatgagatcg aagccggaat cgagcatttg catcagctgt ataacgtgat
    10201 gcgcaacgat aaacgcgagc caggcaaact cagcgaactg aagtttggtc tggagtgcgg
    10261 tggttctgac ggtctttctg gtattactgc taacccgatg ctggggcgtt tctctgacta
    10321 cgtgattgct aacggcggta ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga
    10381 gcagttgctg atggaccatt gccgcgacga agcaacgttt gaaaaactgg tcaccatggt
    10441 caacgacttc aaacagtact ttattgccca tgaccagccg atctatgaaa acccatcgcc
    10501 ggggaacaaa gcgggcggta tcaccacgct ggaagataaa tcccttggct gtacccagaa
    10561 agcgggttcc agcgtcgtgg ttgacgtgct gcgttacggc gagcgtctga aaacgccggg
    10621 gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc tggcgggtgc
    10681 gggctgccat atggtgctgt tcagtactgg tcgtggcacg ccatacggcg gctttgtacc
    10741 gacggtgaaa atcgctacca acagtgaact ggcggcgaag aaaaaacact ggatcgactt
    10801 tgacgcgggt cagctgatcc acggtaaagc gatgccgcag ttgctggaag aatttatcga
    10861 taccatcgtt gagtttgcca acggtaaaca aacctgcaac gagcgtaacg acttccgcga
    10921 actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt taataaaaaa
    10981 cggcgtttca taatgtgaag cgccgtttgc ttttccatac ctgataagga tttatcatga
    11041 ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt tctgattggt atcacaacat
    11101 ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc gccgttattg
    11161 gcgtacattt ttttcttctg ggcacctatc ccgctggtgc cagtgccatc cttaatgcca
    11221 ttcgtacatt gattacctta cgcacgcgca gcttatgggt aatggcgatt tttattgtgc
    11281 tgactggcgg aattggcctc gcgaagttcc atcatcctgt cgaactattg ccggttatcg
    11341 gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga
    11401 tgtggttttc aacgtgttgc tgggtgattc acaacttctg ggcggggtcg ataggcggca
    11461 cgatgattga gggtagtttt ctgcttatga atggcctgaa tatcattcgt ttctggcgga
    11521 tgcagaaaag gggaattgat ccgtttaaag tagagaaaac ccctcccgcc atagacgaaa
    11581 ggggttaaac aattaattac gcagggcgct atttgccaga cgatcgtctt ccgcctggca
    11641 agctgccgca gtgaacagca cgtcagttga agagttcagc gcggtttcgc aagagtcctg
    11701 caatacgccg ataataaagc cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc
    11761 gaacatatta caggccagtg ggatcagcag caaagaaccc cccgccacgc cggatgcgcc
    11821 acaggcacac agagaagcca ctacgctcaa cagcagcgct gtgggcagat cgaccggaat
    11881 acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat
    11941 attgatggtg gctcccagcg gaatagaaac ggaataggta tcgcgatcca gattcagctt
    12001 ttcacacagt gccatattca ccggaatgtt cgccgcagag ctgcgggtga agaaggcata
    12061 cacgccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac gaattttcca
    12121 ccacaccagc aatgggttaa ccaccagcgc caccagtaac atacagccaa ccagcacgac
    12181 cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg tagaagaaac
    12241 cagaccaaaa ataccaatcg gtgcgaagcg aatgaccagt ttcaccataa aggtgacggc
    12301 attcgacata tcgttgacca ggtttttggt ggtctcgtta ccgtgacgca gtgcgaaacc
    12361 gaggccaatt gcccacacca ggatcccgat gtagttacct ttcagcagcg cgtcgatggg
    12421 gttggaaacc atgctcatta ccagcccgcg catcacttct acaatgcctg acggtggcga
    12481 aatatcaccc gcgctactgg ataagtgcag ggtagaaggg aaggcgaagc tgaaaacgac
    12541 tgcggccaga gcagcagaga aggtacccag cagatagagg aacaaaatag ggcggatatt
    12601 ggttttctgc ccgtgctggt ggttagcaat agatgccatc accagcatca acaccaggat
    12661 gggggcaacg gctttcagtg cgccgacgaa caaagtacct aacagaccaa cagcttccgc
    12721 cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca ggatttgttt
    12781 taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg tagtcatttt
    12841 tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg
    12901 atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa
    12961 atgctggatt ttaaagagag gtcatatttt gcaacttaat ggttacattt atttgacata
    13021 aaaataaagc caacgtgcgg cttccagact gaggactacg tcaaaaaagg actcggtcag
    13081 tcccctcgcc ccgtttgggg agagggttag ggtgagggga acaggccagc atcggtgcga
    13141 aaaattaccc tcaccccgac cctctccctg gaagggagag ggagaaaacg gtgctcatgt
    13201 tgatgacagg gtacacggct taggcagatt aaaaatcatc caacccgctg cttatcatgc
    13261 cgataattca cccaggcgtt gataataaac gtcatcacca ggatgccaaa caccacgccc
    13321 agcgagacgg cgattggaat atggtagaag tcgacaatca gcatcttgat accgataaac
    13381 accagaatca ccgccaggcc atatttgagc atcgagaaac gctctgctac gcccgccagc
    13441 aggaaataca tcgcacgcag gccgaggatc gcaaacaggt ttgaggtcag cacaatgaac
    13501 gggtcagtgg tcacggcgaa gatagccgga atgctatcca cggcaaaaat cacgtcgctc
    13561 aattccacca gaatcagtac cagcatcagc ggtgtggcgt acagcaatcc attcttacgc
    13621 acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg tagccagcgc
    13681 accagcggct tgtcgccaat gcctgattca tcttcatggg cgagcgccat cttcacgccg
    13741 gtaaacagca ggaaggcacc gaagatatac aggatccagt cgaactgcga aatcagccag
    13801 ctgccagtga agatcatgat ggtacgcaga acaatcgccc cgagcacgcc atacaccagc
    13861 acgcggcgtt gtaatgccgc cggaacggag aaatagctga acaacatcag ccagacaaag
    13921 acgttatcga ccgccagcga cttctcaatc agataacccg tgaggaaggc cagcgcctgt
    13981 ggatcggcaa cggcgcgacc ttcggtttgt accagatacc accagaaagc agcgttaaac
    14041 agtaacgaca gcgtcaccca gaccagcgac caggccgcag cctgtttcat ggtcatggca
    14101 tgtgccccac gacgtccctg caacaacagg tcgatagcca gcataatggc gacaacaaca
    14161 gcgaatccgc cccatagcaa cggcgtgccg acagtattca tagaagttcc ttacacataa
    14221 aaaaaacggc caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc
    14281 cggcaaggtc tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc
    14341 ggtgaccgga tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc
    14401 ccctttgcag gtaacaaagt atgacagacc attcagtcag tcaatggtct gttacatacc
    14461 ttttacactg ttttactttg gcaattatag ttttacgcta tctgccggaa aaatcacccc
    14521 agtctggcgg cggatctcgg tcagcagttt ggctgtgatg cgactgaccg ccagccctgg
    14581 atgatccacc agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt
    14641 attaatatgc tgcggctggg tgagatcctg catctggctg ccacgcggca cgaagcacac
    14701 tttctggcat tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct
    14761 tgccaggaca gaatcgctga cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc
    14821 catcaccacc acgccgtggg catctacacc acttgccagt aaactggcgg ttgcctgcac
    14881 gcttttcggc tcaccaaata acgctaccgc cgacgccagg cagtaaaagc cgatatccat
    14941 aattgaaccg ttagagaatg ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg
    15001 ttgataccgc gaggaatact ggcaatagtt gaaaaaaact ttccgcagtt tgccgacttt
    15061 cggcagcgcc tggcgtaaca gatgaaagtt aggcaggctg gccgttttaa atgcctcaaa
    15121 cagcaccacc tgattttcac gcgcgcaggc aatggctgca tccacttccg ccagattcga
    15181 cgccagtggt ttctcacaaa tcacatgaat tttgtggctc aggaaaagtt gcgtctggga
    15241 aaaatgcagg gcgttcgggc tggcaatata caccgcgtcg atagcgtcac tttccgccat
    15301 tgcttccagc gaagtaaaca gatgctcgac agaaaactca ttggcgaagt gctgggcctg
    15361 ttcaaggctg cgggaataaa cggcggttaa cttatattta ccgctttcat gtgcggcctc
    15421 cacgaactgg tgagttatcc agttcgtacc aatcacggcg aaacgtatca taacgcgtgg
    15481 atactccata aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc
    15541 ggatacgcat tatttctgcg ccggacaacc atgctcacta aacggaccaa taatgaccga
    15601 cggcccgaga tattttggct gacgattgaa gattttcgcg tcaatcaccg cgctgactct
    15661 ggcgagtttc tctcgcagac ggacaatctt tccgcgtcct ttttccaggt ctggtgcgtt
    15721 aaacccgata gcatcgatgc cgtattgttt tgccagccag atggcgcgtt ggttatgaaa
    15781 ttcctgcgag ataatggtga tatggttctc cccaaacact ttattggcac gcaccactga
    15841 atcaagcgtt gagaatccgg catagtcaca gaagatcact tttgccggta cgccctttgc
    15901 gatcaaagcc tgctgcatgc ctgacgcttc atcgtaattt ttacgtccgt tatcgccact
    15961 gaccagcagc catttcactt ttccggcgtg gtacaactct gctgcggtat caatacgtcg
    16021 ggtaaagtag cgattacccg gcctggcccc cagcaataag cccacgttac gcgccggaac
    16081 gacgttgacg tcgctccagg tcagctgttt gctggcgttg accattacgc gatcggcgat
    16141 aaaaatggtc gctcctgcca caagtaacag taagcaggca atctttagag tccggcgaga
    16201 aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga
    16261 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    16321 agattttgtc ctgcccaagc gacaactggg ttagccacag tcgggtgtcg aactcaagct
    16381 ggtggtactg gggttccata tgacagcaca actggtaaaa cgctttgtta tgctctttct
    16441 ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc
    16501 gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcacacgag
    16561 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    16621 ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc
    16681 agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    16741 ccagacgctg ttcgttaatc agtgttcgca cctgggaaag tagctgctcg ggataaccct
    16801 gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    16861 acccttttca ggggcaatgt cgaaatttta tcattcagga gggccgatga gccacttaga
    16921 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    16981 acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca cagaaatccg
    17041 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    17101 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    17161 cctcaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    17221 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    17281 gcgtgcgctg cgcaaagtgg tcacgccaca aacacgtatt attgccggtg ccaaagcccg
    17341 tgacatccac acttccacgc tggaactatt cgaaaaagtg ctcggcccga ccaccaccac
    17401 gctggcatgg aagaaagcgc gcctgattaa ctgcactttc aatgaaccac cgctggccga
    17461 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc acaaccatgc
    17521 gaatgtcttc tcccgcaccg ggctggatat cggcgcgcgc ttctttatgc aacatctgcc
    17581 agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac
    17641 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    17701 tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gctgcgaatt
    17761 tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa
    17821 cccgccgttt catcagcaac atgcgctgac cgataacgtc gcctgggaga tgttccacca
    17881 cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc gtcacctgga
    17941 ttacttccat aaactgaaga aaattttcgg caactgcacc accatcgcca cgaataataa
    18001 atttgtggtg ctgaaagcag tgaagctggg gcgtcgtcgg taagattgac agctacctgc
    18061 cagataaggc gtaaacgcct tatctggcat acgtttagca cattaccccc aggtcggata
    18121 agatgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc gctccataaa
    18181 acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgttgttt aacgtttatt
    18241 cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt atcatattgc
    18301 caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca ttgaaagatg
    18361 ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctagggaac acgattgcta
    18421 agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg gataacttca
    18481 aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt gttgtagcaa
    18541 tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat aaagaatacg
    18601 atttttttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg ccgacatctt
    18661 gcatgcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc agaacgagga
    18721 acaatacatc caggcgctgg aactggtaga tcatctgctg ctcaacgatc ctgaaaaccc
    18781 cttactggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc ccgaatttgc
    18841 ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc gtacccttat
    18901 ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat ctatggtgtc
    18961 acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat tggcaacgcg
    19021 attcggcatt tctcccgcct tgtttattga ttaacgcga
//
LOCUS       DAZKFZ010000072        19036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000072 DAZKFZ010000000
VERSION     DAZKFZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..163)
                     /locus_tag="ACHOQW_002372"
     CDS             complement(<1..163)
                     /locus_tag="ACHOQW_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573444.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEENYRRALYIL
                     SIIYPIKLNV"
     gene            complement(309..4211)
                     /gene="hrpA"
                     /locus_tag="ACHOQW_002373"
     CDS             complement(309..4211)
                     /gene="hrpA"
                     /locus_tag="ACHOQW_002373"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP9573445.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4412..5017
                     /gene="azoR"
                     /locus_tag="ACHOQW_002374"
     CDS             4412..5017
                     /gene="azoR"
                     /locus_tag="ACHOQW_002374"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP9573446.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5068..6360)
                     /gene="ynbD"
                     /locus_tag="ACHOQW_002375"
     CDS             complement(5068..6360)
                     /gene="ynbD"
                     /locus_tag="ACHOQW_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP9573447.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6374..8131)
                     /gene="ynbC"
                     /locus_tag="ACHOQW_002376"
     CDS             complement(6374..8131)
                     /gene="ynbC"
                     /locus_tag="ACHOQW_002376"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP9573448.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8147..9043)
                     /gene="ynbB"
                     /locus_tag="ACHOQW_002377"
     CDS             complement(8147..9043)
                     /gene="ynbB"
                     /locus_tag="ACHOQW_002377"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9573449.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(9043..9648)
                     /gene="ynbA"
                     /locus_tag="ACHOQW_002378"
     CDS             complement(9043..9648)
                     /gene="ynbA"
                     /locus_tag="ACHOQW_002378"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9573450.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9819..12125)
                     /gene="ydbD"
                     /locus_tag="ACHOQW_002379"
     CDS             complement(9819..12125)
                     /gene="ydbD"
                     /locus_tag="ACHOQW_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFP9573451.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGGSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12189..13049)
                     /gene="pdxI"
                     /locus_tag="ACHOQW_002380"
     CDS             complement(12189..13049)
                     /gene="pdxI"
                     /locus_tag="ACHOQW_002380"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP9573452.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13269..15728)
                     /locus_tag="ACHOQW_002381"
     CDS             complement(13269..15728)
                     /locus_tag="ACHOQW_002381"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9573453.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(15948..18983)
                     /locus_tag="ACHOQW_002382"
     CDS             complement(15948..18983)
                     /locus_tag="ACHOQW_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFP9573454.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVP
                     PDDGGDTPVKHNPVVYKNGVTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIV
                     KSWTVHDDSNTVVFDGVNTSGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAII
                     TDQGGNTALNGGTVMTVDGDNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVD
                     GGTAVIINGDNTKLNTAGDSTITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVG
                     DGATIKQEGDLYVSGGAHGIDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMG
                     DLNVGQAAAGENAIGIQIDGDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVG
                     DFSTGLDVSGNNNNMTLATYELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNA
                     QPYFFNPSTGVNISGDNNDVTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDD
                     NTFTLNGGVHFVGEKNVMDDGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFP
                     LGFENLIQLSHGAELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVEL
                     KNIGFAAAWNKDSTVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQ
                     HSVLNYPAEWNLSDGTSFNNKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAID
                     KSTADNEGQITLDTLWVDENDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATAT
                     NHENGVITVYNAGAGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGT
                     AINDQTGVININAENGQAFYNDGTGLIINYGTICTFGVC"
BASE COUNT         4791 a         5120 c         4306 g         4819 t
ORIGIN      
        1 cgacgttaag tttaatcgga taaattattg ataaaatata cagcgcgcgt cggtaatttt
       61 cctctggaag tttttcatag aaagatatta aagcctgacc aacgcgatca ccctctatta
      121 tccgttcaaa ttgcgacctc agtaaaatat catgtctact cattgcaata cgtcctgttc
      181 gcccaaggcg aagtatgaat gaaacttatg taaaatagta ccgcccttgc tatatttcag
      241 aattcataaa gaaaaaaccc ggcgctaaat gcttaccggg ttttagattt atcaggcaaa
      301 tagcagggtt aaccgctaat ctgctccatc gcctgcaaaa tacgcttatc tgaaatcgga
      361 taaggcgtac caagttgttg agcgaagtaa ctaacgcgca gctcttctat catccaacgg
      421 atctctttca cgtcttcatc ctcacgacgt gcaggcggga gtttgttgaa ccattgctgc
      481 cacgcctgct ggacgttttc gactttcagc atctgtgcac gatcgcgatg tggatcaacc
      541 gccagttttt ccagccgttt ttcaatcgcc tgcaaataac gcagcgtgtc gcccagccgt
      601 ttgaagccgt taccagtgac aaaaccgcga tataccaacc cgcccatctg cgctttaatg
      661 tcagaaagcc ccagcgccat ggtcatatcc acccgccctt tcagacgttt gttgatattg
      721 aacactgccg taaggatttg ctcgacctgc ttcgcaatat ccaccaccgt gtcgttcagt
      781 tcggcacgca ctttttcatg cagcgcagca aagccttctt ccgtccagac cgggcctcca
      841 ttggcgtcga tcagttgatc cacaccgcat gagatacagt cgtcgatcag ctccagcact
      901 ttgccatacg ggttaaagta cagccccagc ttggctttgt ttggtaactt ctcatgtaaa
      961 tacttgattg gtgagggaat attcagcagc agtagacggc gaagaccgtt ccacattgcc
     1021 tgtttttgct ccagcgggtt atcaaacagt ttgatcgcca cactgtcgcg ctcatccacc
     1081 agcgccggcc acgccttcac tttgtagtta ccacgcttct gttcgtagct ttccggcaac
     1141 tgaccaaaac tccagatatg taagccgctc tgctcgatac cgtcatccgc caccgcagat
     1201 agcgtttcct gcactttgcc tttcagcgca tctttcagat cttgtagcga gcgcccttct
     1261 tttagcttct tgtttttgtc atccaccacg cggaaggtga ttttcaggtg atcgggcacc
     1321 tgatcccagt gccagtcttc gcggtcaacg gtaacgccgg tcatccgccg taactcgcgc
     1381 tcaaggctgt cgagcaacgg taactccagc ggtgtgacgc ggcctaaaaa cgcttcggca
     1441 tagtttggcg caggtacaaa attacggcgt accggtttcg gcaacgattt aatcagagca
     1501 atcaccagtt cgcggcgcag accagggatc tgccattcaa acccgctttc ctcaacctgg
     1561 ttaagtaacg gcagcggaat atgtacggtc acaccgtcag catccgcgcc gggctcaaac
     1621 tgatagctca aacgcagctt gagattgccc tgatgccaga agttcgggta atccagcttg
     1681 ctgatctttt ccgccccttc tttgatcaac atgctctttt caaagttgag caaatcaggc
     1741 gtttcgcggc tgactttttt ccaccagctg tcgaagtgac gagcggagat tacatcgtgg
     1801 ccgatgcgct ggtcgtagaa ctcaaacagc gtttcgtcat caaccagaat atcgcggcga
     1861 cgtgatttgt gttcaagctc ttccacttcg gcccgcagtt tcaggttttc acggaagaat
     1921 gcgtgacgcg tctgccagtc accttccacc agcgcgtggc gaataaagag ttcacgacat
     1981 aacgccggat cgatctggct gtagttgacc ttgcgcgcgg caacaatcgg caaaccataa
     2041 acagtgactt tttccgttgc catcaccgcg ccctgcgccc gttcccagtg cggttcgctg
     2101 taggtacgtt taatcaaatg ctgagcgaca ggctccaccc attccgggtc gatacgcgca
     2161 gcaatgcgcc cccacaggcg gctggtttct accagttccg ccaccattac ccatttcggc
     2221 ggttttttga ataaaccaga accggggaag atggagaaac gcgcgttacg tgcgccggta
     2281 tattcttgtt tatcggcatc tttcatgccg atatgggaaa gcaaccccgt cagcaacgca
     2341 atgtgaattt cacgatactc cgccggttcg ctgttcaccg gaatgccgag ttctttcacc
     2401 acctggcgca actgggtgta gatatcctgc cattcgcgca cacgcagata gttgagataa
     2461 tcggtacgac acaggcgacg gaaggcgttg gaagaaagcg ccttttgctg ctcgccaaga
     2521 taattccaca gattcacaaa cgcgaggaag tcagactctt tgtcgtggaa gcgacgatgt
     2581 ttttcgtccg atgcctgctg tttgtccatc ggacgttcgc gcggatcctg aatggagagc
     2641 gcagacgtga taatcatcgc ctcacgcacg cagccatgtt tttgcgcttc cagcaccata
     2701 cgcgccagac gtgggtcgac aggcaactgc gagagctggc gaccgagcgg cgtcagttta
     2761 taggcgctgg cctgttcatc cgtggtgatc gcgcccagtt cttcgagcag acgcacgcca
     2821 tcctggatat tgcgtttatc cggtgcttcg acaaacggga acgcagcgat atcgcctagc
     2881 cccagcgcgg tcatctgcaa aataaccgag gccaggttgg tacgcagaat ctccggatcg
     2941 gtaaactccg ggcgcgagag gaaatcgtct tcggaataga gacgaataca gatcccttcg
     3001 gacacacgac cacagcggcc tttacgctga ttggcagacg cctgcgaaat gggctcaatc
     3061 ggcaaacgct gcactttggt gcgatagctg tagcggctga tacgcgccgt accggggtcg
     3121 ataacgtatt taatccccgg tacagtcagc gaggtttccg cgacgttggt cgccagcaca
     3181 atgcgtcgcc cgctgtgcga ctggaacacg cggttctgtt cgctattcga aagccgcgca
     3241 taaagcggca agatttcggt atggcgtaag ttcagcttgt tcagcgcatc ggcggtatcg
     3301 cgaatttccc gctcgccgct cataaagatc agaatgtcgc cggggctttc ctggcttagt
     3361 tcgtccacgg catcgaaaat tgcctgcaac tggtcgcgct cagtgtcgtc ggcttcttca
     3421 acaatcgggc gatagcgcac ttccaccgga taggtccgac cggagacttc aataatcggc
     3481 gcattattaa agtggcgcga aaagcgttcc gggtcgatag tcgcggaagt gataatgatt
     3541 tttaggtcag gacgccgcgg cagcaactct ttcaaataac cgagcagaaa atcgatattc
     3601 aggctacgtt cgtgcgcttc gtcaataatg atagtgtcgt actgcatcag caggcggtct
     3661 tgctggatct ccgccagcag gataccgtcg gtcattagct tgaccatcgt gttatcactt
     3721 acgtgatcgc tgaaacgcac tttgtaaccg atgcaaccgc ccggctccgt tttcagctct
     3781 tccgcaatac ggttcgctac cgttcttgcc gccaggcgac gcggctgggt atggccgatc
     3841 agccctttaa tcccgcgccc cagctccata cagattttcg gtaactgagt cgttttacca
     3901 gaacccgttt ccccggcgac tatcaccacc tggtgatcac gaatcgcttc gagaatgtcc
     3961 tgttttttct gactaaccgg caaattgtca ggataagtaa tttccggtcg tgccgcttca
     4021 cgcagcagga ctttccctgc cgcctggtca atctctttcg ccatctcctg gaaaatggcc
     4081 tgttgtgcat caggattttt aaccttcttc acgccgtgca gacggcgaga aaaacgcagt
     4141 ctgtcacgca gcatcagcga atccagccgc tgctgcaagg ccgtaaaagt caatttttgt
     4201 tgttctgtca taacgttagt gggcagtagt ctgcctgatt aattttcttt gaaatgatag
     4261 atatagagta ccacatcgcg gctttctatg cgttgttcaa taaattcgaa cataggcttc
     4321 gatttattgc gctatctctg tggcaggatt gtgaataaag tgtcaacaag caacggggca
     4381 tcgcccattc aaatatctat aaggaaacac catgagcaag gtattagttc ttaaatccag
     4441 catcctggca gggtactctc agtctaatca gttgtccgat tattttgttg aacaatggcg
     4501 cgaaaagcac tccgctgatg aaatcaccgt tcgcgacctg gctgcaaatc caattccggt
     4561 actggatggc gaactggttg gcgctctgcg tccgagcgat gcgccgctga ctacgcgtca
     4621 gcaggaagct ctggcacttt ccgatgagct gattgccgag ctgaaagccc atgacgttat
     4681 cgttattgcg gcaccgatgt ataacttcaa catctcgact cagctgaaaa actattttga
     4741 cctggttgca cgtgcaggcg ttactttccg ctataccgag aatggtccgg aaggtctggt
     4801 aacgggtaaa aaagccatcg ttattaccag ccgcggtggg atccacaaag atggaccaac
     4861 ggacctggtg acgccgtatc tgtccacgtt cctgggcttt atcggcatta ccgatgtgaa
     4921 atttgtcttc gccgaaggga tcgcatacgg tccggaaatg gcagcgaaag cgcagtctga
     4981 cgcgaaagca gccatcgaca gtattgttgc tgcataaccg gacacgatct tgatgtggac
     5041 tgtagttcag tccacatctc aatccactta cctgttttcc cataatttca acatcgcttt
     5101 gtgctcttcc ttaagcacaa tatgcgagcg tctggctcga ataaagctaa tcgcttcatc
     5161 aactgtttta cagtgtccgt aacataacag ccatgccgcc actaccagcg cactgcgcga
     5221 taatcccaat gcgcaatgga ccagaacgct gccttgctct tcgcgtaatg tttccagcat
     5281 cgccacggcc tgtcggagct ccccctcttc cggaaccacc agatccagca tcggtacaca
     5341 gaaatagagt cgatcttttg tcgctcgtcc gcgagggaat tcaaaggtga catccagaac
     5401 cgcattctgt gccggaatat gtcgtggaaa cgcccctaaa taaacaccag cagtcatttt
     5461 gctcaccggc tccaggcgac gacaaaacca acgcatagat agccacatcc cgatgcgcca
     5521 gggcaatgtc agccagtaaa cggcgggcgg gagtttcccc tgactatctt tccctgttgt
     5581 tatcgcgcca agcccaccgt acccacggcc aataatgagt agcgataata ctggccaaca
     5641 taaccagact gaccaccata actgaaccat catcattagc tccatcaaca caatgcacgc
     5701 gcacgcgcct acgacatatg gcagtgctat tttgattcga cgttgatcag gtttctgata
     5761 attccaacga cggtcgacgg gtatcatcca gtcaatcaac atacctaccg ccagccctgt
     5821 gatgacatca ataaaatgat gctgccaggt tgtcagcatc gaaatggcga tgagtaaaaa
     5881 ccatccgccg cagactttac gccacctcac agccagatgc tgacgaaagt gacgccaaag
     5941 tagccagcag agaacaatat gcagcgaagg agactggtta taaggcagat caaacagttc
     6001 aagttgcgaa aatagccatc ctgtcacccc actcacttca ggacggataa aactaaactt
     6061 cagcgggtag agaaaaaaac cgcagcaggc cattaccgtt gccagaataa gccggtggac
     6121 aagtcggcgc tgttcgaatg tcgagctaca aacgaacagc gaaaatccat ataaaagatc
     6181 cagactccag taagggacaa tagtccaggg aagaaaaggg atcgccgttt cccagccgaa
     6241 tacctgactg gggatatcat ggctgttaaa gtcctgaacc gcggtgaact gatttagaga
     6301 tccataggtg aagaagaaaa acggggccag caacaataac cagccagcgc cttgtagcaa
     6361 tacgttccgg cgttcagtta tcacgacgca ccgccatcga aaccgtaaaa atgccccact
     6421 catcaatccg ttgtgtgcat ttatcaaatc cggcatcacg cacgagtgaa tccatctccc
     6481 cttgcgaacg cacgcgcatt acccacggtt taccatcttt atgactggtt aacaccccgg
     6541 caatcagctc cagttgtggg tgccacggct gcccggtgta gaggagaatg ccgcccggtt
     6601 cgatggcatt ggcaagacct gcgagtgagt tttttacctg ctcatttccg ggaaaaagct
     6661 catacagtcc agagacaatc gccagcgtag gccgcggagt taacgcgctg agttcctccg
     6721 ggttaaacgc atcgccctgt tcaaaacgca cccgcccaga cattccccgt tgagcaatca
     6781 tctcttgccc ctgtgcaaca tttaactcgc tgtaatcacg taacaaaata tcgcttacgg
     6841 caggctcgtt tgccagcgca tccagtacat agcgcccatg ccctgcggca atgtcaacca
     6901 cgcggacggc taaacctttg gcgtggagat cggcaacggc ttgtttaatc agtatttgta
     6961 aatgggtttt gcgctggcga ataccgcgcc agccaacact gttcaggtag attttgtcga
     7021 ttaagcgccc gaatgcgtta ctaccttgcg gttgattttg atagacataa tccagcgaac
     7081 tgcctgaatc aaagccggtg ctcattccga gatgcaggcc cgcagagtgc gtcccgaaca
     7141 atttcatcgc tttacgcata aagcgatacg ccaaatcaac cggcgataat ggcacgggtc
     7201 cacctgaaag gagccgccag cgatccgctg atggtccagt gcggtcttca tgctgataat
     7261 caaatttttg tgacttgtta gcatataaac gactaataaa gctttgcatt ttttcaaatg
     7321 cctgcgccct gttctcttca cccaacgtgt cgtgataaaa gcctggcagc agatgcagct
     7381 ctttcagagg gctacgtaat ctatgataaa aatcaatctg cggttggcga tgcaccacat
     7441 agtcatcgcc tgatatcaga agttgcgtgg ggagcgtaat cgccgccgca tcgctaacaa
     7501 tacgttcaga cgttttataa agatcgagca agatattaac ggcaatcgcc cgtgtgatca
     7561 gcggatcatt attgaaactc gccacccgtt gccgatcgtg ggtcaaatag cgtcctttca
     7621 cataggaatt aataaaaaac agaccacgca gacgatgcca taacgccagc gcaggacgtg
     7681 ccagcggcac atacaattta accttaaagg ccggagaagc cagcaccagc ccgcgaattg
     7741 caggcgcata atcatgaacc catgtggcaa ccagcactgc gccgacgctc tgcgcgatca
     7801 ctaccacctc ttccagcccg acctggctgt cgctggcagc aaaacggaca aactcatcga
     7861 catcccgcac tgaacgcgca agagatgggc tataaccacg gggccccgaa gtttgtccat
     7921 gccctcgggc atcccatgca taaaaagcag tatctggcat cgccagttca tcaacgatat
     7981 gttgtagacg accagaatgt tcatgaccgc gatgaaataa gacgatgacc tttttcgccc
     8041 ctggctgtaa agcgggccag tgccgataaa acaacgctgt attatcactg gtggtaaaaa
     8101 aatgttcccc agggatgcgt gaattttcca ttttctctat tttccttcag taacagcagt
     8161 agcgaataaa ataaaaaaat accggagcgg tgaaaattaa tgagtcaatc ctgtcaagga
     8221 gtccgccatg tcctggtaat agttttccgc tatctttaac acccacatct cgtttgacgg
     8281 ctgacatcac tacatcgccg caaaaaccac taataccaat taacaaaccc gctaataatg
     8341 cctgtaatgt atttagcggt gtcagcaacg gtccaataat cagtgacgca atcatggtgg
     8401 tgattacgcc ccccaccaga ccttccagcg tttttcccgg gctgactttg gggaccactt
     8461 ttcttctgcc gcaggatttc ccccataaat actgtgcaat atcgtttgac tctgttaacg
     8521 ccaaaagaaa taacaccagt aacgccccgc cctgggtatt tgtggttgtc aacattaata
     8581 accaggcggc atgactcaag gcgaaaacgg tcattatcca gccccaaaaa atggcgctca
     8641 cggtatgcag aaaaccggag gtatcaccta ctaaaactcg ccaggtggtt aatatcagaa
     8701 acccagccaa aggtataaat aacagaaaca gctcaaagca actaaaaccg ataagcaaat
     8761 agttaagagg aataacccaa tataaccaac gcggaaaatg tacggatata agcgtacaat
     8821 attctttcaa tgccagaaaa cttatcagcg caaaaaacgt caacgtcatc cagcgtggac
     8881 ctgaaatcac cagtgaaaaa caaatgacga taccccacca ggtacgaata cgtagtgtaa
     8941 cttcatcccc ttttctctcc ggtaaccgcc atacccgaaa acggtttatc agcgttgcta
     9001 aaattaacaa cgcgaaaagt gttgccagag atttttccag cattatcttt cagcactcat
     9061 caggagcaca ctccgacaac gattaatcgc agtccagaga agcagaattg aagcaatgct
     9121 ccataaaaga ttattccatt gcatccactg tggataaatg gcaacggcca gcccccacaa
     9181 cccaaatatt aacgcgcgat cgcttttgcc aaaaggaccg acataactac gaataccgtt
     9241 aatcgtctgc gcgagtaaac cgcaaaactc agtcaatatg gtgcaaaata acatgagtat
     9301 tacgagtgat gcgttacttt ccggtaaaaa taaaaacggt aaatagagcg caatatcgga
     9361 aataacatcg ccagtttcat ttaaaatcgc ccccagacgt gtttgctgat tgcactcacg
     9421 cgccaacatg ccatccagcg cattgagtgc catacggata aaaagcacga tgggcaatag
     9481 caaaaagagg atgggttgtg cgaccaacat caacagcaat ccggtaataa gagaaagcgc
     9541 cagtgcagca agagtgatgt gattcgctgt aacatggtgc ttataaagcc aaaacatcgt
     9601 cggccttaac agcgactgaa agagcggttt tatctggtag agcgtcatta tcaatccttg
     9661 ataattcggt ttgcgctatg gtgcatcaat tcccctgacg gagatatcgc aacacgacgc
     9721 ttcattaaaa attcagaata tatcaaggta ttggggactc actgtcagga atgattatcc
     9781 ccaataaatc aagtggttaa aaattattac gctgttattc aatacaactc atcaagtttt
     9841 ctcatcgctt gttcccgtat tgcgcgcgtc ttaccgttaa gcgccagctg gcgaactaaa
     9901 tccagttcag ggttaacaag ctttaacgcc agcaaaggat cacgtttcag cctcgccttt
     9961 aatacatcaa ctggaaaacg gggattattc atcgccacaa tccaccactc gggatcaatt
    10021 tctgccgcca gtactgcagg cggtgtgtgg cggttgttaa gtgcgccgaa cttgaaaaat
    10081 gtgagattgc ttttcgccag tgtactccac catatacgat ccggttgttt tgccagtaca
    10141 tccagttgtg ctaacgcatc ggggtctagt gcataataaa cacgtaacgc ttcgttaacc
    10201 tgcgcacggg aactgttttc ctgcctcaga aaaaacttcg cccaaatatc attgcgtggc
    10261 gtgttttgct gtaattcctt gagaacaaca gcgggtaaat tatcccaact aattaatgca
    10321 ctctctaaaa agtcccagtc gtccgaacgt atcagagagg tggcatttaa tagcatctgg
    10381 tcatcaactt caccgtagct gataagctca gacaaaatca tatcagcgct ggtttcatat
    10441 gcatctgaat ctgctttggg attatttttc cgatgcgata aagcacgttg ccaaattttt
    10501 tgtttcatcg acgtgctcag atactgattt ttcgccagat tactcccccc ctcaggatcg
    10561 ttgaaacgtg tcaataaccg ttgtaaagca gcgtcagagt gaattatctg catatcccga
    10621 cagcaaaagt aggcagggga ttttttctcc atccttttaa caaaattaaa ctgtaagtta
    10681 tctggcaacg ctaaaaataa cgcacctatt agatcatcgt cattctcgaa tttttggtat
    10741 aaatatactg ctattttgtc actctcttgt ggcgtcatcc gcaacaattg accatggtgt
    10801 ttcatctctg ccagctttaa cgctaagctt gtgacaacat cgtttgatga atcgttcgcc
    10861 aactgcattg cctgattgta ggtgagttgt cctccagcac cgacctgacg aatagaggaa
    10921 tcatcactgc ggatatagcc atcaataatg gattcgggca ataaccacgc atctttataa
    10981 agctcctgaa tgatcatatc ccaatctctt ttcgccagag tcatgacgat atccagaggg
    11041 agattttcac gtagcgcaag attcaacgga ccgacaccat caggtagatg actcattcgc
    11101 gtcattattt cgacagagag cggttcacgg tcgagcaacc ccagaacaac aggctggtaa
    11161 cccaattgct gatcggtcaa ctccagaatc tcattgtcag gcatatatct cgccatactt
    11221 tcaatcgttt gtccatcatt gctattaata agttgtttgc gtaggttagc tgaaattcct
    11281 ttaagccttg ggagagttcg cattatggca agatcaaatg tcgcaatatc tcgtccttca
    11341 aatgcagcaa cgttgccttc atccagcatt tgcaattcct gcactaactc aggagaaggc
    11401 ttaaggcgaa acagcgcttc ttttttcatc tgattacaga cattccctgc catgcgaata
    11461 ttgcttcgct taaaaatttc tttcagagca tcttcatcat ttattagacc ccaataatat
    11521 gcatcaggat ttattatcat cagcgctttt ttgacatttt cagtgtcatt tttgtcactc
    11581 gcacggacca tgaattcact atttgtcgaa gacgcaacaa caggtgcaat tatgtcattg
    11641 ataaatatcc cacgcgcatt aatactcccc ttaatcgtag acggattatc gtaatatgag
    11701 tcgataaata ccagtccact tgcatttatc gaaccggtaa ttaacagtgg tgaatccgta
    11761 actatattcg ttgccgtcac gttaccgaga ataacccatg gattagaaag agagaaagtg
    11821 gaaatattac ctttgactgt caaattgcca agaacaatca atttttgatt actctctata
    11881 ttaccttcta ccactaaatc gccacgatgc actttccagt ccgatgcaga taaaccgaag
    11941 gggacaagat tcacgctact gttgatagct tgttcaactt gttgtttatc ttcagcggta
    12001 gccgcagaaa aaaaaggttc aatatcaact gcatttgcaa cgcatcgaag ttccgcctgt
    12061 actggaagac tcagcataaa caggggaagt aaacctatcc agctgcaaag cctgggctta
    12121 aacatatgca ctccatgcca ttaaaaaaga aacaaaaggg aaatgcatct ttcccttttg
    12181 tatattcgtt attctcgcga aataccatcc aacgtagaca acactttctc agaaagatgc
    12241 aacttttcag cagccatatt ctcccgtaaa tgcgcaacag aagacgtccc tgggatcagc
    12301 aaaatattcg gtgaacgttg taacagccac gccagcgcca cctgcattgg tgttgcaccc
    12361 aagctcgcag caacatcgga aagcgtggaa gattgcagcg gcgtaaagcc cccgagcgga
    12421 aagaacggca cgtaggcaat gccgtcacga gccaaagctt caatcattgc gtcatcagca
    12481 cgatgagcaa tgttgtattc gttctgcaca cagacgattt cggcaatctt gcgcgcctct
    12541 gcaacctgcg tcggtgtgac gttgcttagg ccaatatgtt ttaccaggcc ttgttgttgc
    12601 atctctgcca gcacggtcag gctggcttca atcgatcctt ccgccgggcc atgaccatcc
    12661 cccatcatca cgcgcaggtt aaccacatcc agtacgtcca ggccgagatt acgtagatta
    12721 tcgtgcaccg ctttttgcag ctctgccgga gaaaatgcgg gcaaccagga tgcgtcctct
    12781 ccacgccgcg caccaatttt agtgacaatt gtcagatcgt cagagtaagg ataaagcgct
    12841 tcgcggataa tctgattggt gacgtgcgga ccataaaagt cgctggtatc aatgtgattg
    12901 acgcccaatg ccagcgcctc acgcagcacg gttatagcga cgtgacgatc tcttgggggg
    12961 ccaaaaaccc caggacctgc cagttgcatc gcgccataac caagacggtt aacggatttt
    13021 gtaccgagag taaatgtatt gctgctcatc gcaccctgcc ctgtaataaa acggattaag
    13081 aaaaatcagt atagaaacga aaagagataa ataatgcgaa ggcgtaaaca agatttctgg
    13141 atgagtagca ctattgtcct gaaagacacc aaaacattag acagcaaaag ataagtcaag
    13201 cgtcgcatcc ggcaataaaa aaccgcaacc tgtatcagcg ttgcggtttg tgtatcacat
    13261 gctaaaaatt agaacgtttt cttaaagttc agcatcacgc ctttgtcgct gatgccgtcc
    13321 tctttccagt gatacgcatc cagattcagc gaactttctt tgctactgta cttcacgccg
    13381 acgctcgcca ggctgttgat accgccgccc ttctgaccgt catcgacgtt aaagtgctgg
    13441 ctgcctgccc ctgcaaggct tgccgtgcgc tggctcttgc tgtagctcag gttcggcccg
    13501 ccttccagcg tcgcattcgc gctccagccg cctttccctg cgtagtccag tttcagcccg
    13561 acgatgctgt ccaccgccgt ttcgctgccg ctgttcatgc tcaggttaaa gtctccggca
    13621 ttgcgctcct gatagccgcc ttccagcgag tgacgcagtt tcactccggc gtacggggtg
    13681 attttcagcc cttcgcgcgg ctcaaaggtt ttcgcccctt cgctacgcaa ctccaggtac
    13741 tgctgtttca catccgtatc cgccgttttg ctgacgtcgc cgtaagcgac cgagcggctg
    13801 ctgtcgagat tatgcacgtc ataacgcagc gcgttgttcc aacgcatacc attgtcgaag
    13861 gacatctggt gcttcaggcc aaagaactgg ctgtagccgc cggttacgcc attgtcgccc
    13921 gctttctgcg caccgtcgcc atccagacgc gcgataccgt attccaggct catgctctgg
    13981 ctttcgctca ggtcaacggt tttacgcagt gccagcatgt catactcggt gttatttccg
    14041 agttccgcac gcgggtcacc tttcgccacc acgttaaagg ccaggccatt gcccaccttc
    14101 ggcgcggcat ccgccagcat gctgaagcgg ttgcttaaca cacgcgcttc acggaatacc
    14161 gtggtcgcct ggctaccgct cacctgcttc agggcgctat tcagttcagc agtggtgccc
    14221 acgttcaggc tggtatacag ctcgttattg gtgtaacccg catccagtgc cttcgccaca
    14281 tcgttcaccg aagcatcggt cgccacatcg gtgtaggcgt ttttgctcat gatgacgtca
    14341 acgttgccgc tggcatcggt gctgcctttg gcggtccata ccacggacgt tgaggtgatg
    14401 gcatcggcgt cggtcaggtt gatgccttcc accacgttgt cgaaactgac cgtggtgtct
    14461 gccgtaccgg cggcgaaacc ggtgttgatc ccgacaccat tcatgctggc gttgttgacc
    14521 ttcagtttac cggcgctgcc gtcaacgttg gtaccgacga tatagccgtt gaggttgtta
    14581 ctgctgccat cgctactacc gctatcagtc gtttcactga aaggtgaagt ggtgtaagtg
    14641 tttggcatgt ccggcaacgt gtagtcggtg taatgcactt cggtattgtt accgctgttg
    14701 ccgttcaccc cttccacatt cacgctgtcg ccctgcttaa tcaaccccga accagtcacg
    14761 ccgtcggcaa tcaccaccgt accgttgtta acgatatgac cttctgtgcc cagtacgctg
    14821 aacgcaaacg agtcgttagc aaaaatattg atcgtgccgt tgttctcaat taccgcatcg
    14881 gcggtggcat tggcatccag ttgcatacca gtcaatccgg tatcggtggt gccttcggta
    14941 ccgagattga taacgccgtt gttaaccagc tgtgcgtttt tatcgccttc catcgccacg
    15001 gcgttgttgc cgctgatgtt cattacacca tcgttgagag caacatagtt gttgtgggtg
    15061 aatttaaccg cgctggcgtt gtctttatcg aagttaatta ccccgccatc ctggttccac
    15121 aagaaggcat cgctttctgt cgcaccggca gtacttaccg ccacggtgac tttcgcatcc
    15181 accacgccct tgttaataaa ctcgctggcg ccatcgtcgt agtgaatagc gcccgcacct
    15241 aatgcggcat ccagcgtcat ttcaccggtg ttatataacg tggcgttagc attgctgccc
    15301 tggaagcgaa tggcgttttt gctgttatca aggatgagtt tgccgctgtt gttgaacgtt
    15361 gacttatcgt caatatccag cgtcccggca ttgttgtttt catcctgata acccattttc
    15421 caggtacccg cgttattcac taccgcagag ttatagagaa tcgcgccttt acgcgcctcg
    15481 ccgcctgcaa ggttattgaa taccgcatcg ccctgagcgt taacgcgcgc cgacgcaata
    15541 ccgctgttat tcaccgtacc atcgtgaagt tgtaaagttg acagggaacc ggtgttatta
    15601 attaccgcgt tgctgttttt ctcaattttt acgacgtcat taagcgttcc gttgttattg
    15661 aaggttcctc cttccagagt caccgtgttc aatacacctt cattggttaa cgcaccgcct
    15721 tcattcacga cgatggctga gctaatggtt ccgctcgact cgttaaccag cgcaccattt
    15781 tcgagtatcc tgatcgttcc atttttaaca gcacccgcac tggtgacatc cccgttgata
    15841 atcgcacctg acgataataa tacggtctca cccgcagaac ttaatacagc gccatcatca
    15901 tataaatcag caatataatt atccgttgga ttatattcat cggcccccta gcaaacgcca
    15961 aaggtacaaa ttgtgccata gttgataata agcccagtgc catcgttata gaatgcctga
    16021 ccattttccg cgttaatgtt gattacgcct gtctggtcat taatggcggt gccttcgtga
    16081 taaaccgcca tgccgacgag tttattcaca ccgagagaat cgttgtaatt ttcatttttc
    16141 tccaggttga tggttccctg gttaatgacc gtattagtat tcccatacgc cgccatcccc
    16201 gcaccggcgt tgtagacagt aatcacacca ttctcgtggt tggttgccgt ggctttagtt
    16261 aatgccccat tataggcatc ggtacctgaa gccattccca caccgaaatc tttcgccgtg
    16321 ctatttgcaa cattattacg cagcgatgtt tcatcatttt catcaaccca cagcgtatcc
    16381 agggtgattt gcccttcgtt atccgcggtt gatttatcga tggctagcat ccctacactg
    16441 ccacggccat acatatcaat aatacccgtt tcgccgttca gaatggatga cgagtagctg
    16501 gcaagcatac ctgtcagccc taatgccttg ttattgaaag aggtaccatc agacaagttc
    16561 cactctgccg gataattcag cacactgtgc tgattcatca ttttggtggt catggtacca
    16621 ttgttaacgc caatcccacc gtattcaaca tcaaagactt ttggtgccgg gtcggtacca
    16681 aaatcgtaca tcgccaggcc aatggatccg ttattgatta ccgttgaatc tttattccag
    16741 gcagccgcaa aaccgatatt tttcagttcc acttcaccgt tgtttgtcgc cttcgcacca
    16801 gaatcaatac taatggtgga taacgctaca cgataataat aagtgaaaga atccacatca
    16861 ctcatatcaa acgttgcgtc agcgccaatc tccagctccg cgccatggct caactgaatg
    16921 agattttcga accccagcgg gaactcgccg ctgatggttg actcaccatt caggtagacc
    16981 ggggaatgcc catcaacgtt aataagcggt gtgtctccga taccacgtcg agaagctgat
    17041 ggttttgagc catcatccat gacatttttt tcaccgacaa aatggacacc gccattaagc
    17101 gtaaaggtat tgtcatcgcc agcaataaca atacctgcaa tgtgttcctg agcaccgtcg
    17161 taactcgcat aggaacgcga cgtggtttta ctatcagcaa caacggttaa ctgaccatcc
    17221 agcgtgacgt cgttgttgtc accgctgata tttaccccgg ttgagggatt aaagaagtaa
    17281 ggctgcgcat tatcggcttt ctgatcttta tcaaccagaa tactgcccgc tatttcgata
    17341 gtattaccat cacccgaaac attaacaccc gtcgcttttt gccctgtcac attcagctca
    17401 taagtcgcca gcgtcatgtt gttattattg ccagagacat caagaccggt ggagaaatcc
    17461 ccaacgtcta acccacccgc gagtgaaata ttagctttat ccccagcaac gctgacaccg
    17521 gttccggcat tggtcgcgga aatatcacca acgttaacaa atgtcgcatt atcaccgtcg
    17581 atctgaatgc caatggcatt ttcacccgct gcggcctgac caacattgag gtcgcccatg
    17641 ttgatcacgc tgacgccatc accatcaagc agcatgccaa tagagttatc ttcgatgacc
    17701 gtaatattac ctttgttgct aacgaacgta tcattgccgt caacgtcaat accgtgcgca
    17761 ccaccgctta cgtataagtc gccttcctgt ttgatggtgg caccgtcacc gacaattttc
    17821 gagccagtgg aattctctcc gtcaacgctc atggtacctt gattatcgac ccgcgcatta
    17881 tcgccattga taaggctacc cgtaccacca ttggtgatgg tcgagtcgcc cgcagtgttt
    17941 agcttggtat tatcgccatt gatgattacc gcggtaccgc catcaacttc cgtctcgcca
    18001 ttattgttaa tggtgatatt gtcgccagtg agtataccga caaccgagcc ttctccgatg
    18061 gccgtcgttt tgccgtcgtt gttgagggag atgttgtcac catcaaccgt catgacagta
    18121 ccgccattca gcgccgtgtt gccgccctga tcggtgatga tcgcgttacc aaactcaatc
    18181 gcattcccgg ttgtgccgtc aacaacgacg ccggcctctt tagtgatgtg gatgagtcca
    18241 tcatcgtcgt aactccaggt gatgccacct gaggtattta cgccatcaaa gacaacggtg
    18301 ttgctgtcgt catggactgt ccaggactta actatcgtct ccttgccctc aggcgccgtt
    18361 aaggtgtagc tcccatcggc attttggctg taggtaaagg tagtttcgcg aattttgaca
    18421 gtttttgcat cctgatccca ggttacgcca tttttataga cgacaggatt gtgtttaacc
    18481 ggggtatcgc caccatcgtc tggcggaact ggcgtatcac cgccgtcatc tggcggaacc
    18541 ggcgtatcac cgccgtcatc aggcggaact ggcgtatcac cgccgtcgtc tggcggaaca
    18601 ggcgtatcac cgccatcatc cggaactggc gtatcaccgc cgtcgtctgg cggaacaggc
    18661 gtatcaccgc catcatccgg aactggggag ttattatggt ggtgattgtc atcgtcactt
    18721 aaggtatata tagcaattcc cgtagctact gcagcaagac ctgcagctgc ccatggtaaa
    18781 actgcactat ctttattttc tctgcactcc gcggataaat tttctagctg ctctttgctc
    18841 agggaatgaa tatttgccgg acatgttatc ttactggact tacgtgtttt ttcggttgag
    18901 tcgggtattg aagtatccgc agcccatgtc tggctactta acgccatggc aacacaaaca
    18961 gacaataatt ttttcctttg catataaaat tccttagatc tagaccgtgc gtaacaagcc
    19021 atgcagaatt tgaaga
//
LOCUS       DAZKFZ010000073        18698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000073 DAZKFZ010000000
VERSION     DAZKFZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            145..813
                     /locus_tag="ACHOQW_002383"
     CDS             145..813
                     /locus_tag="ACHOQW_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9573455.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            857..5623
                     /gene="ehaG"
                     /locus_tag="ACHOQW_002384"
     CDS             857..5623
                     /gene="ehaG"
                     /locus_tag="ACHOQW_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP9573456.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5991..7646
                     /gene="lldP"
                     /locus_tag="ACHOQW_002385"
     CDS             5991..7646
                     /gene="lldP"
                     /locus_tag="ACHOQW_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9573457.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7646..8422
                     /gene="lldR"
                     /locus_tag="ACHOQW_002386"
     CDS             7646..8422
                     /gene="lldR"
                     /locus_tag="ACHOQW_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP9573458.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8419..9609
                     /gene="lldD"
                     /locus_tag="ACHOQW_002387"
     CDS             8419..9609
                     /gene="lldD"
                     /locus_tag="ACHOQW_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP9573459.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9795..10268
                     /gene="trmL"
                     /locus_tag="ACHOQW_002388"
     CDS             9795..10268
                     /gene="trmL"
                     /locus_tag="ACHOQW_002388"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9573460.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10321..11142)
                     /gene="cysE"
                     /locus_tag="ACHOQW_002389"
     CDS             complement(10321..11142)
                     /gene="cysE"
                     /locus_tag="ACHOQW_002389"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9573461.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11222..12241)
                     /gene="gpsA"
                     /locus_tag="ACHOQW_002390"
     CDS             complement(11222..12241)
                     /gene="gpsA"
                     /locus_tag="ACHOQW_002390"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9573462.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12241..12708)
                     /gene="secB"
                     /locus_tag="ACHOQW_002391"
     CDS             complement(12241..12708)
                     /gene="secB"
                     /locus_tag="ACHOQW_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP9573463.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12771..13022)
                     /gene="grxC"
                     /locus_tag="ACHOQW_002392"
     CDS             complement(12771..13022)
                     /gene="grxC"
                     /locus_tag="ACHOQW_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP9573464.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13164..13595)
                     /gene="yibN"
                     /locus_tag="ACHOQW_002393"
     CDS             complement(13164..13595)
                     /gene="yibN"
                     /locus_tag="ACHOQW_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9573465.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13840..15384
                     /gene="gpmM"
                     /locus_tag="ACHOQW_002394"
                     /gene_synonym="pgmI"
     CDS             13840..15384
                     /gene="gpmM"
                     /locus_tag="ACHOQW_002394"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP9573466.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15418..16677
                     /gene="envC"
                     /locus_tag="ACHOQW_002395"
     CDS             15418..16677
                     /gene="envC"
                     /locus_tag="ACHOQW_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP9573467.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16681..17640
                     /gene="yibQ"
                     /locus_tag="ACHOQW_002396"
     CDS             16681..17640
                     /gene="yibQ"
                     /locus_tag="ACHOQW_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP9573468.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17627..18662)
                     /gene="waaH"
                     /locus_tag="ACHOQW_002397"
                     /pseudo
     CDS             complement(17627..18662)
                     /gene="waaH"
                     /locus_tag="ACHOQW_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
BASE COUNT         4709 a         4820 c         4852 g         4317 t
ORIGIN      
        1 tgaatgctta aaacaatatt taatcgacac acatcactgt cattaatgta aagcaacata
       61 gatcaccaat aatacaatgg acaactctac cggcattgat cgctgggcgg aggaaataag
      121 gatttttaca tgaactagaa gaagattttt ttcagtgctg tcactgtttc agttctctgc
      181 gcattaacgg gatgtgacta cattgaagaa ggcaagccag aaagtagttt attgaaacaa
      241 caagaagaac ataacaataa aattgatcta cttgaaaaac aacaagcaca gctaaagagt
      301 caactggaaa ctattcaaaa acaacaaact ggcataataa gcagtacaaa gactttaact
      361 cacgtaatta aatccgttaa agatcaacaa aacactttta tttttaccga gtttaaccca
      421 gcaaaaacga agtatttcat cctgaacaat ggttcggtgg ctttagcggg tcgagtgtta
      481 tccattgacg ccacagagaa tggcagtgtt attcatattt cactggtcaa cttattaagt
      541 acacctatct caaatattgg ttttaatgcg acatggggtg gtgaaaaacc tgtcgatgcc
      601 aaagagtttg cccggtggca acaattactt ttcaacacat caatgaaatc cacattgaaa
      661 ttattaccag gtcaatggca agacattaat ttgaccctga agggtgtatc gcccaataac
      721 ctgggatatc tgaagttagc catcaacatg gaaaatattc agttcgacaa tcttccctct
      781 gctgaaaatc ggcagaaaag aagcaaaaaa taaatgcagt tagtacgctt ataacaagga
      841 atagcgagca tcattaatga acaaaatatt taaagttatc tggaaccctg cgacagggaa
      901 ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag aaatctgggc gcagtaagct
      961 gttaatttct gcgctggttg cgggtggaat gttgtcgtcg tttggggcat tggcgaatgc
     1021 cgggaatgac aacggtcagg gtgttgatta cggtagtgga tcagctggcg acggctgggt
     1081 tgctataggc aaaggggcga aagcaaatac ttttatgaac accagtggtt ccagtactgc
     1141 tgtgggttat gacgctatag ctgaaggcca atatagctct gccatcgggt caaaaaccca
     1201 tgcgattggt ggtgcatcaa tggcctttgg ggttagtgca atatcagaag gcgatagaag
     1261 tatagcactg ggtgcctctt cgtattcatt gggccaatac tcaatggccc tcggccgtta
     1321 ttcaaaagca ttgggtaaat tgtctattgc tatgggggac tcttccaaag cggaaggagc
     1381 aaacgccatt gccctgggaa atgccactaa agctactgag attatgagta ttgctcttgg
     1441 cgacaccgcc aatgcgtcaa aagcgtattc aatggcgctg ggagcaagta gcgtcgcatc
     1501 tgaagaaaac gctattgcga taggtgctga gaccgaagcc gctgaaaatg caactgctat
     1561 tggcaataat gcgaaggcaa aagggactaa tagcatggca atggggttcg gaagccttgc
     1621 cgataaagtc aatactatcg cattaggaaa tggcagccag gctctggcag ataatgcaat
     1681 cgccataggc cagggcaaca aagctgatgg cgtggatgcc atcgctctgg gtaatggtag
     1741 ccagtcgaga ggcttaaaca ccattgcctt aggcacagcc agtaatgcaa ctggtgataa
     1801 gagtcttgcg cttggtagta atagcagtgc caacggtatt aactctgtcg cgctgggcgc
     1861 agattccatt gcggatttag acaataccgt ctctgtcggc aatagttcat taaaacgcaa
     1921 gatcgttaat gtgaaaaatg gcgcgatcaa gtctgacagt tacgatgcca ttaatggttc
     1981 acagctttat gccattagcg actcggtagc aaaaaggctt ggaggagggg ctgcagtaga
     2041 tgttgatgac ggtactgtta cagcaccaac ctacaattta aaaaatggta gcaaaaataa
     2101 cgtaggggct gcgctcgctg tacttgatga aaacaccctg caatgggacc aaaccaaagg
     2161 caaatacagc gctgctcatg gtactagtag cccaactgcc agcgtaatca ccgatgttgc
     2221 ggatggcacg atttcagcct ccagtaagga tgcggttaac ggttcccaac tgaaagctac
     2281 caatgacgat gtcgaagcca acaccgccaa tatcgctact aataccagca acattgccac
     2341 gaatacggca aatattgcca ccaataccac caatatcacc aacctgacgg attccgttgg
     2401 tgaccttcag gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca
     2461 cggccaggat accaccagca aaatcaccaa cgttaaagat gccgacctga cggctgacag
     2521 cactgatgct gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac
     2581 caccaatatc gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct
     2641 gactgagacg gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt
     2701 attcacggca gctcatggca ccgagaccac cagcaaaatc accaacgtta aagatggcga
     2761 cctgacgact ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc
     2821 cgtggcgacg aataccacca atatcgccac taacaccacc aacatctcta atctgactga
     2881 gacggtgact aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac
     2941 tgcagctcat ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac
     3001 tgcaaccagt tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc
     3061 cacctacttc ggcggcaatg cttctgtgaa tactgacggt gtgtttaccg gtccaaccta
     3121 caaaatcggt gaaacaaatt attataacgt cggcgatgca ctggctgcga ttaactcctc
     3181 atttagcacg tctctcggcg atgctctgct ttgggatgcc accgcaggta aattcagtgc
     3241 caaacacggt actaatggtg acgcaagcgt gatcactgat gtcgcagatg gtgaaatttc
     3301 agactccagt tctgacgcag taaacggctc acaactccac ggcgtgagca gttatgttgt
     3361 tgatgcgctg gggggtggtg ccgaagtcaa tgcagacggc accatcactg cgccgacgta
     3421 caccattgct aatgctgatt acgataatgt cggtgatgcc ctgaatgcta tcgataccac
     3481 tcttgacgac gctctgctct gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc
     3541 tcacggaaaa gataaaactg ccagtgtaat cactaacgtc gctaacggtg caatctctgc
     3601 tgccagcagc gacgcgatta acggctcaca actctatacc accaataagt acatcgctga
     3661 tgcgctgggt ggtgacgcag aagtcaacgc tgacggcacc atcaccgcac cgacttacac
     3721 cattgcgaac gccgagtaca acaacgtcgg tgacgccctg gatgcgcttg atgataacgc
     3781 cctgctgtgg gatgagactg ccaatggcgg tgctggagcc tacaatgcca gccatgacgg
     3841 taaagccagc atcatcacta atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc
     3901 agtgaacggt tctcagttga atgcgacgaa tatgatgatt gagcagaaca cccaaattat
     3961 caatcagctc gctggtaaca ccgacgcaac ctatatccaa gaaaacggtg cgggtattaa
     4021 ctatgtgcgt actaacgacg acggcttagc gttcaacgac gccagcgcac agggtgttgg
     4081 cgctacagct ataggttata actctgtcgc caaaggcgat agcagcgtag ctattggtca
     4141 gggcagctac agcgacgttg atacgggtat cgccctgggt agcagctctg tttccagccg
     4201 agtgattgcc aaaggctccc gtgacaccag cataacggaa aatggcgttg ttattggtta
     4261 cgacaccacg gatggcgaac tgctcggtgc attgtctatc ggtgatgacg gtaaatatcg
     4321 tcaaatcatc aacgtagccg atggttccga agcccatgac gccgttacgg ttcgtcaatt
     4381 gcagaatgcg attggtgcgg tcgcaaccac gccgactaaa tacttccacg ctaattcaac
     4441 ggaagaagat tcactggcag tgggaactga ctcgctggca atgggtgcga aaaccatcgt
     4501 gaatggcgat aaaggtattg gtatcggtta tggtgcctac gtggacgcga atgcacttaa
     4561 cggcattgcc attggtagca atgcgcaagt cattcatgtc aacagtattg cgataggtaa
     4621 tggttctacg accactcgtg gcgctcaaac caattatacc gcctacaaca tggacgcacc
     4681 gcagaactct gtcggtgaat tctcagtcgg tagtgcggat ggtcaacgtc agatcactaa
     4741 cgtcgcagca ggttcggctg ataccgatgc ggtcaacgtg ggtcagttga aagtaacgga
     4801 tgcgcaggtt tcccagaata cccagagcat tactaacctg gataatcggg taacgaatct
     4861 tgattcacgc gtcaccaata tcgaaaacgg tattggcgat atcgtcacca ccggtagcac
     4921 caagtacttc aagaccaata ccgatggtgt agatgccagc gcgcagggta aagatagcgt
     4981 cgcgattggt tccggctcca ttgctgccgc tgacaacagc gtcgctctgg gtacagggtc
     5041 tgtggcaacc gaagaaaata cgatctctgt aggttcctct actaaccaac gtcgtatcac
     5101 caacgtagct gcaggtaaaa atgctaccga tgctgttaac gtggcacagt tgaagtcttc
     5161 cgaagctggc ggtgtacgtt acgacaccaa agctgatggt tctatcgact atagcaatat
     5221 caccctcggt ggcggcaacg gcggtacgac tcgtatcagc aacgtctccg ctggcgtcaa
     5281 caacaacgac gtggtgaatt acgcgcagtt gaagcaaagc gtgcaggaaa cgaagcaata
     5341 caccgatcag cgaatggttg agatggataa caaactgtct aaaactgaaa gcaagttgag
     5401 cggtggtatc gcttctgcaa tggcaatgac cggtctgccg caggcttaca ctccaggtgc
     5461 cagcatggcc tctattggtg gcggtactta caacggtgaa tcggcagttg ctttaggtgt
     5521 atcgatggtg agcgccaatg gtcgttgggt ctacaaatta caaggtagta ccaatagcca
     5581 gggtgaatac tccgccgcac tcggtgccgg tattcagtgg taatgatcca ttaagttagt
     5641 gtgactaagg gcaaacgcca aattgcctga tgcgttacgc gcatcaggca agataaggcg
     5701 ctccgggtca acaatctaag ggcaattctc tgatgaggat tgcccttttc tttaccagac
     5761 atctcccccc acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa
     5821 ttccccaatt aaaactcaca tcaatattgc caatacataa catttagtta accattcatt
     5881 gtcattatcc ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag
     5941 atgagcaaca gactcattac acgatgtgcg tggactccag gagacctgca atgaatctct
     6001 ggcaacaaaa ctacgatccc gccgggaata tctggctttc cagtttgata gcatcgcttc
     6061 ccatcctgtt tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct
     6121 catggacggt agcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta
     6181 acgcgctggc ctcggtggtt tatggcttct tctatggttt gtggcccatc gcgtggatca
     6241 ttattgcagc agtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc
     6301 gctcgtctat tctttcgata acccctgacc agcgtctgca aatgctgatc gtcggtttct
     6361 gtttcggcgc tttccttgaa ggagccgcag gctttggcgc accggtagca attaccgccg
     6421 cattgctggt cggcctgggt tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta
     6481 acaccgcgcc agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa
     6541 caggcatcga cagctttgag attggccaga tggtggggcg gcagctaccg tttatgacca
     6601 ttatcgtgct gttctggatc atggcgatta tggacggctg gcgcggtatc aaggagacgt
     6661 ggcctgcggt cgtggttgcg ggcggctcgt ttgccatcgc tcagtacctt agctctaact
     6721 tcattgggcc ggagctgccg gacattatct cttcgctggt atcactgctc tgcctgacgc
     6781 tgttcctcaa acgctggcag ccagtgcgcg tattccgttt tggtgatttg ggggcgtcac
     6841 aggttgatat gacgctagcc cacaccggtt acactgcggg tcaggtgtta cgtgcctgga
     6901 caccgttcct gttcctgaca gctaccgtaa cactgtggag tatcccgccg tttaaagccc
     6961 tgttcgcatc gggtggcgcg ctgtatgagt gggtgatcaa cattccggtg ccgtacctcg
     7021 ataaactggt tgcccgtatg ccgccagtgg tcagcgaggc tacagcctat gccgccgtgt
     7081 ttaagtttga ctggttctct gccaccggca ccgccattct gtttgctgca ctgctctcga
     7141 ttgtctggct gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acgctgaaag
     7201 aactggctct acccatctac tccatcggca tggtgctggc gttcgccttt atctcgaact
     7261 attccggact gtcatcaaca ctggcactgg cactggcgca caccggtcat gcattcacct
     7321 tcttctcgcc gttcctcggc tggctggggg tattcctgac cgggtcggat acctcatcta
     7381 acgccctgtt cgccgcgctg caagccaccg cagcacaaca aattggcgtc tctgatctgt
     7441 tgctggttgc cgccaatacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta
     7501 tcgctatcgc ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta
     7561 ctgtcaaaca cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt
     7621 atgtcttaac gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt
     7681 tgccgatcgt gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc
     7741 cgctgagcgc caactggcga tgcaactcgg cgtatcacgt aattcactac gcgaggcgct
     7801 ggcaaaactg gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg
     7861 ctggcgtcat gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc
     7921 cgatgatccg gattacagtt tcgatattct ggaagcccgc tacgccattg aagccagcac
     7981 cgcatggcat gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt
     8041 tgaagcaacg ctaagtgaag acccggatat cgcctcacaa gcggacgttc gttttcatct
     8101 ggcgattgcc gaagcctcac ataacatcgt gctgctgcaa accatacgcg gtttcttcga
     8161 tgtcctgcaa tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt
     8221 ttcacaactg accgaacaac atcaggctgt cattgacgcc atttttgctg gtgatgccga
     8281 cggggcgcgt aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt
     8341 cgatgaagat caggctcgcc acgcacggat tacccgcctg cccggtgagc ataatgagca
     8401 ttcgagggag aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa
     8461 cgcattctgc cgccgttcct gttccactat atggatggtg gtgcatattc tgaatacacg
     8521 ctgcgccgca acgtggaaga tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac
     8581 atgtctgact taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca
     8641 ctggcaccgg tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa
     8701 gcggcggacg cgcatggcat tccgtttact ctctcaacgg tttccgtttg cccgattgaa
     8761 gaagtcgcgc cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc
     8821 ggctttatgc gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc
     8881 accgtggata tgccgacacc gggagcgcgt tatcgtgatg cgcattctgg gatgagcggc
     8941 ccaaatgcgg caatgcgccg ctacttgcaa gcggtgacgc atccgcaatg ggcgtgggat
     9001 gtgggcctga acggtcgtcc gcatgattta ggtaatattt cggcttacct cggcaaaccg
     9061 accggactgg aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg
     9121 aaagaccttg agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc
     9181 gatccggaag atgcgcgcga tgcagtacgt tttggtgctg atgggattgt agtttctaac
     9241 cacggtggcc gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca
     9301 gatgcggtga aaggtgatat cgccattctg gcggatagcg gaattcgtaa cgggcttgat
     9361 gtcgtgcgta tgattgcgct cggtgccgac accgtactgc tgggtcgtgc tttcctgtat
     9421 gcgctggcaa cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgagaaagag
     9481 atgaaagtgg caatgacgct gactggcgcg aaatcgatca gtgaaattac gcaagattcg
     9541 ctggtgcagg ggctgggtaa agaattgcct acggcactgg ctccgatggc gaaagggaat
     9601 gcggcatagt cgtttgagcc ccctcaccct aaccctctcc ctcagggagt ggggaccgtt
     9661 cggcgctgta tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc
     9721 cctccggggt gaggggaaaa ggccgcactg ctcaccaatt ttctgctatc ctgcccccgc
     9781 actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg
     9841 gcaatatcat ccgtctttgc gctaataccg gctttcgtct gcatatcatc gaaccgatgg
     9901 gatttgcctg ggacgataag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg
     9961 ccgttacgcg tcatcatgac tttcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt
    10021 ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aaactatcag gatggcgatt
    10081 atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat gccctgcccg
    10141 ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaac ctgtccaatg
    10201 cggtgtcagt tgtggtgtat gaagcctggc ggcagctggg atatccggga gcgttgttga
    10261 gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga tcacaggaca
    10321 ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct ggtccatatc
    10381 cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc cagcggcggt
    10441 tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc
    10501 aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt
    10561 cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga
    10621 tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat
    10681 aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca cagaaacctg
    10741 gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt gaccgatgcg
    10801 ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt atttatcgac
    10861 tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga tcatttccgg
    10921 gtcagcggcg taggcttctt ccaccacttc acggatagca atagcaggca taattggcga
    10981 tgacagcttg ttcgccagca tgtagctcag tgcactacca aggttttcgt gcttgagtag
    11041 cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc tggcttcggc
    11101 tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg
    11161 agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg
    11221 gttagtggct gctgcgctcg tccttgcgtg cacgacctaa taacgtcaat gctgcctcgc
    11281 gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc atttctacgc
    11341 cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc accacctgac
    11401 caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcatg ccaaaacggc
    11461 ggttacgcga ctggttgtcg gtacaagtaa gcacaagatc gcccagcccc gccatgccca
    11521 taaaggtggc aggatcggca cccagcgccg cgccaagacg cgacatttca gccagcccac
    11581 gggtgatcag tgccgtacgc gcattcgcac caaaaccgat accgtcggac atccccgcgc
    11641 caatggcaat gacgtttttc accgcaccgc caagctgcac gccaatgaaa tccggattgc
    11701 tgtaaacgcg gaaacttttg ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct
    11761 gatcggtcga ggccagcgaa atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg
    11821 ttgggccaga gataaccgcc agcggaattt gatcgcctaa ggcctcacgc gccacgtcct
    11881 gtaacagacg tccggtttcc gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag
    11941 gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg ggtacgacga
    12001 cgagaatatt acggctggct gccagcgcag tggcgagatc gctttcaaga tggagcgtat
    12061 cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga
    12121 tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc agggtgatgg
    12181 caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta cgttggttca
    12241 tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat agttcatgaa
    12301 cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg aatgtaccgc gggataccat
    12361 gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg cgcccaggca
    12421 atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct gctgaacttc
    12481 acacaggaac gcggtttctt cgcccaggga ggccgttacg gtaacacgca gtaccacttc
    12541 gtatacgtca tctgccagtt gggaagatgc cgtatccaga tcaagtttaa cttctggttg
    12601 ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagagatat ccttggtata
    12661 aatacgttgg atctggaaag tcatttcagt gttgttttgt tctgacatgt gtagaaaacc
    12721 cttaagtgtt gtccttaaat actgcgcaat gtaatgccaa cgttcacacg ttatttcagc
    12781 aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc gccaatgtgc
    12841 tgtgcgtcaa taaaaatctg gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca
    12901 cgcttggcgg cattgccatc gatcggcaac tcctgaaaac tcacgccctt gctgctcagc
    12961 agtgcttttg cacgatggca atacgggcag gtttctttgg tatagatttc aacattggcc
    13021 ataatttagc tcctgttttt ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg
    13081 agtgtcccca gaagcttata acaataagtc gctgggggcc taattgcagc taacggcctg
    13141 catcatgaaa gacgacaggt aaattatttg ccgcgcacca aaggcaagtt ttcgccagcc
    13201 cagccagcga cgccttcttt cagcacgaat acttgcgcaa aaccagcttt cgtcagtgcg
    13261 tttgcaggct cctggcactg catgccagaa ccgtctacca cgataaccgg tttgtctttg
    13321 tgcttctcaa gctcaccaac attgttggct ttgatttcgc tcggcaacag attgatagaa
    13381 cctgcgatat ggcctttacg gaagtcatca cgctgacgta aatccacaac cacagcgtct
    13441 tctttgttga tcagacgcgt agcttcacca cgagtaatca ccttcacttt cgaggtcagg
    13501 ctcttaaacg tagtcacaag aaccgccacc agtaacgcga tccaggcgat actcagtatg
    13561 ggatgacggc caacaaattg cataatttct tgcatggggg gtaacaactc ccgacgtagt
    13621 gattaaaaaa accaggaaag gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt
    13681 aactggaatg caattttgcg gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg
    13741 ccgcgagcgg tcgtatattt tgatcttcag aggctatttt atcgattcag ctgtagtaaa
    13801 attacgcaaa ttttgactct tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct
    13861 atggtactgg tgattctgga tggctatggc tatcgtgaag aacagcagga taacgccatt
    13921 tttagtgcta aaaccccggt aatggatgca ctgtgggcca atcgtccgca taccctgatc
    13981 gacgcttccg gtctggaagt cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc
    14041 cacgttaacc tgggtgccgg ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa
    14101 atcaaagatc gcgctttctt tgctaatcca gtgctgactg gcgcggtaga taaagcgaaa
    14161 aacgcaggta aagcagtaca cattatgggt ctgctctccg caggcggcgt acacagccac
    14221 gaagatcaca tcatggcgat ggtagaactg gcagctgaac gcggcgcaga aaaaatctac
    14281 ctgcacgcat tccttgacgg tcgcgacacc ccgccgcgca gtgctgaatc ctcgctgaaa
    14341 aaatttgaag agaaatttgc tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt
    14401 tactacgcga tggaccgcga taaccgttgg gatcgcgttg aaaaagccta tgacctgctg
    14461 actctggcgc agggtgagtt ccaggccgat actgccgttg ctggtttgca ggcagcttat
    14521 gctcgcgacg aaaacgacga gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca
    14581 gatgcggcaa tggaagacgg cgatgcgctg attttcatga acttccgtgc tgaccgcgcg
    14641 cgcgaaatca ctcgtgcatt cgtaaacgca gatttcgacg gcttcgcgcg taagaaagtg
    14701 gttaacgtcg atttcgtgat gctgaccgaa tacgccgctg acatcaaaac tgcggttgct
    14761 tacccacccg cttccctggt taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact
    14821 cagttgcgta tttccgaaac cgaaaaatat gcccacgtta ctttcttctt caacggtggc
    14881 gtagaagagt cgttcaaagg cgaagatcgc attctgatca actcaccgaa agtggctacc
    14941 tacgatctgc aaccggaaat gagctccgca gagctgaccg aaaaactggt tgcggccatc
    15001 aagagcggca aatacgacac cattatctgt aactatccga acggcgacat ggtaggtcac
    15061 accggggtga tggaagcggc ggttaaagcg gttgaagcac tggatcactg cgtggaagaa
    15121 gtcgcgaaag cggttgagtc cgtgggtgga caactgctga tcaccgctga ccacggtaac
    15181 gctgaacaga tgcgcgatcc ggcaacgggt caggcacaca cggcacacac caacctgcca
    15241 gttccgctga tttacgttgg tgataagaac gtgaaagcgg ttgcaggcgg caaactttct
    15301 gacatcgcgc cgaccatgtt gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt
    15361 aagccgctgt tcatcgtgga ataatccctc cccatgaggg gaaaggcgat taataccatg
    15421 acacgggccg tgaaaccgcg caggtttgca atcaggccca tcatctacgc cagcgttctt
    15481 agcgctggcg tattgttgtg cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa
    15541 tctattcagg ccgatatcgc cgcaaaagag cgcgcggtac gccaaaagca acaacaacgc
    15601 gcaagcctgc tcgcacaatt gaaaaagcag gaagaagcga tctctgaagc cacccgtaag
    15661 ctgcgcgaaa cgcaaaacac gctcaatcaa ctcaataaac agattgatga gatgaacgcg
    15721 tcgattgcca aactggagca gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg
    15781 gatgccgcat tccgtcaggg cgagcatacc ggtattcagc tgattctcag cggtgaagaa
    15841 agccagcgtg gacagcgttt acaggcttat ttcggctatc tcaaccaggc gcgacaagaa
    15901 accattgccc agttgaagca aacgcgtgaa gaagtcgcca tgcagcgtgc tgaactggaa
    15961 gagaaacaga gcgagcaaca aacgctgtta tatgagcagc gcgcccaaca ggcgaaactg
    16021 actcaggcgc tgaacgagcg taaaaagacg ctggcagggc tggagtcttc catccagcaa
    16081 ggtcagcaac agttgagcga gctgcgcgcc aacgaatccc gtctgcgtaa cagcattgcc
    16141 cgtgcggaag ccgcggcgaa agcgcgtgca gaacgagaag cacgtgaggc ccaggcggtt
    16201 cgcgaccgcc agaaagaagc gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa
    16261 aaatcgctga tgtcccgaac tggtggcctg ggggcgccgc gtggtcaggc attctggccg
    16321 gttcgcgggc cgacgctgca tcgctatggt gaacagctac agggcgaact acgctggaaa
    16381 ggaatggtta tcggtgcctc tgaaggtact gaagttaaag cgattgccga tggtcgggtg
    16441 attctggctg actggctgca aggttacggt ctggtggtgg tggttgagca cggtaaaggc
    16501 gacatgagtc tttacggcta taatcagagc gcactggtga gcgttggttc gcaggttcgc
    16561 gcgggccagc caattgcact ggtgggcagc agtggcggtc agggtcggcc ttcactctat
    16621 ttcgaaattc gccgccaagg tcaggcggtc aatccacagc cgtggttggg aagataagtt
    16681 ttgtttccat ttcgtcgtaa cgttcttgca tttgccgctc tgttggcgct ctcctccccc
    16741 gtacttgctg gcaaacttgc catcgtcatt gatgattttg ggtatcgccc gcacaacgaa
    16801 aaccaggtgc tggcgatgcc ctccgctatc tccgtcgctg tattacccga ttcaccgcac
    16861 gccagagaga tggcgaccaa agcgcataac agcgggcacg aagtgttgat tcatctcccg
    16921 atggcaccac tgagtaaaca gccgctggag aaaaatacgc tgcgcccgga gatgagcagc
    16981 gacgaaattg agcgcattat tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc
    17041 aacaaccaca tgggtagcaa gatgacctct aacctgtttg gtatgcagaa agtgatgcag
    17101 gcactggagc gttacaatct ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg
    17161 atgcgcgccg cgcaaggtac tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat
    17221 tcgcagaacg aagcggacat ccgtgtgcaa tttaatcgcg caattgatct ggcgcgtcgc
    17281 aacggttcaa ccattgccat tgggcatcct cacccttcaa cggtacgcgt gctacaacag
    17341 atggtttata acctgccgcc agacattacg ctggtgaaag ccagcagctt gctgaatgaa
    17401 ccgcaggttg atacttctac accgccgaaa aacgctgtgc ctgatgcacc gcgtaatccg
    17461 ttccgtggcg tgaagctgtg caaaccgaag aaaccgatag aacctgttta tgctaatcgc
    17521 ttctttgaag tattaagcga aagcatcagc cagagcacgc tgatcgttta cttccagcat
    17581 cagtggcaag gctggggcaa acagcccgaa gcggcgaagt ttaacgctag cgcaaattaa
    17641 aggcgctacg cgtaatgcga tggtgcgacc gcgttttttt cgcgccattg ccataagcgg
    17701 aaagaccaca gtaacgcctg gtaaccgacc ttcacgctgc gcacattggt aatcaggcgc
    17761 ttatacatac cggaagtaaa tatctcggca atcatccgtt ggcgggtaag aatatccggc
    17821 tctttgcgca ccgcatggca aacacgcaat gcttcgtaag ttatttgctg atgaaattcc
    17881 ggataaatca taattttgtc ggcataattt cgatttaatt tctccagcag gcgggtaatc
    17941 ttaatatagt gacgttgata attaaggttt ttattccctt gtctatgcaa ccgactcact
    18001 gacgtattat gcagataata tttatataat gattgctcgg tatatcgcgc acgcagcgcg
    18061 ttaaacatga attctgttgt ccagacaata tcctgatgat gtaatccggc aataaattta
    18121 atgttatttt taacaataac atcacggcga taaaccccca tccagacaac gtgagtccaa
    18181 cggcgcgaag aaagccccat ccgcagccag tccgggccgg ttaatacacc ggttgagcga
    18241 aggcgatcgc tggggatgga ttgccaggtt tctcccgttt cacgaaaaga ccagtcagcg
    18301 ttgcactgcg ccacgtcgag gtcgtcctct aacgccatgg tcatcagcgt ttcgtacatg
    18361 gtgggataga cctcatcgtc agcatcgaca aaagcgacat atttgcccgt cgccacctcg
    18421 atcccacgat tacgcgctac cgatgcgcca gcattcgcct gatgcaacaa acgaacgtgc
    18481 ggatagtttt ctgcgtaatg ctttgctatt tcaacagaat tatccgttga accatcgtta
    18541 ataataatga tttccagagc agtccaggtt tgcgtaatta aagattccat acaagtgcgg
    18601 aaatcatcgc ccgcattata taacggaata ataacactaa gtttattggt gctgttcatc
    18661 ataaattacc gacgacctct gaagttatcc tgacgttt
//
LOCUS       DAZKFZ010000074        18630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000074 DAZKFZ010000000
VERSION     DAZKFZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(24..212)
                     /gene="bcsR"
                     /locus_tag="ACHOQW_002398"
     CDS             complement(24..212)
                     /gene="bcsR"
                     /locus_tag="ACHOQW_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP9573469.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            485..2056
                     /gene="bcsE"
                     /locus_tag="ACHOQW_002399"
     CDS             485..2056
                     /gene="bcsE"
                     /locus_tag="ACHOQW_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP9573470.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQYS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2053..2244
                     /gene="bcsF"
                     /locus_tag="ACHOQW_002400"
     CDS             2053..2244
                     /gene="bcsF"
                     /locus_tag="ACHOQW_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP9573471.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2241..3920
                     /gene="bcsG"
                     /locus_tag="ACHOQW_002401"
     CDS             2241..3920
                     /gene="bcsG"
                     /locus_tag="ACHOQW_002401"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP9573472.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(4007..4114)
                     /locus_tag="ACHOQW_002402"
     CDS             complement(4007..4114)
                     /locus_tag="ACHOQW_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9573473.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            4590..5861
                     /locus_tag="ACHOQW_002403"
     CDS             4590..5861
                     /locus_tag="ACHOQW_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP9573474.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            complement(5891..6895)
                     /gene="dppF"
                     /locus_tag="ACHOQW_002404"
     CDS             complement(5891..6895)
                     /gene="dppF"
                     /locus_tag="ACHOQW_002404"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP9573475.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(6892..7875)
                     /gene="dppD"
                     /locus_tag="ACHOQW_002405"
     CDS             complement(6892..7875)
                     /gene="dppD"
                     /locus_tag="ACHOQW_002405"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP9573476.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(7886..8788)
                     /gene="dppC"
                     /locus_tag="ACHOQW_002406"
     CDS             complement(7886..8788)
                     /gene="dppC"
                     /locus_tag="ACHOQW_002406"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP9573477.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(8798..9817)
                     /gene="dppB"
                     /locus_tag="ACHOQW_002407"
     CDS             complement(8798..9817)
                     /gene="dppB"
                     /locus_tag="ACHOQW_002407"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP9573478.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(9968..11575)
                     /gene="dppA"
                     /locus_tag="ACHOQW_002408"
     CDS             complement(9968..11575)
                     /gene="dppA"
                     /locus_tag="ACHOQW_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP9573479.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(12320..12396)
                     /locus_tag="ACHOQW_002409"
     tRNA            complement(12320..12396)
                     /locus_tag="ACHOQW_002409"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12360..12362),aa:Pro,seq:cgg)
     gene            complement(12488..14179)
                     /gene="eptB"
                     /locus_tag="ACHOQW_002410"
     CDS             complement(12488..14179)
                     /gene="eptB"
                     /locus_tag="ACHOQW_002410"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP9573480.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            complement(14434..14964)
                     /gene="lpfE"
                     /locus_tag="ACHOQW_002411"
     CDS             complement(14434..14964)
                     /gene="lpfE"
                     /locus_tag="ACHOQW_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP9573481.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            complement(14969..16024)
                     /locus_tag="ACHOQW_002412"
     CDS             complement(14969..16024)
                     /locus_tag="ACHOQW_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9573482.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCPPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            complement(16039..18612)
                     /gene="lpfC"
                     /locus_tag="ACHOQW_002413"
                     /pseudo
     CDS             complement(16039..18612)
                     /gene="lpfC"
                     /locus_tag="ACHOQW_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
BASE COUNT         4466 a         4962 c         4584 g         4618 t
ORIGIN      
        1 cagtacggcc attcaccaat cccctacttt tgttgcgcaa actctgccag caacggccag
       61 cgttttaatg ccgcggccaa ctgttcgcgt tgggaaatat cggcataatc aataccaggc
      121 agtgaaaatg cctgctttaa cgccaaaata tcattctgga agatatagcc tatcgcggga
      181 tcaggcagag tatctggttc attgttattc attttgcttg atccctatga aataaccgta
      241 ccgttcatga gacaattcta accgtgagca tttcgccggt ttattgttta cggcgagacg
      301 ggaatttgct cactttttga ctttccgtta cgcaacagaa aaagttaaaa acgaaaagac
      361 taaaatcaca cgtcttgtga cctgctaaat tgattcacat gctaaatctg ataagtttta
      421 atttcaatgg taggtttatt tcttagcttt cgctagtaaa ctgataatca gacaaaatgg
      481 tgacatgagg gacattgtgg accctgtatt ctctatcggt atctcatcat tatgggatga
      541 gctgcgacat atgccagcag gcggcgtctg gtggtttaac gtcgatcgcc atgaagatgc
      601 tatcagtctg gcgaatcaaa caattgcatc ccaggctgaa accgcacacg tcgcggtcat
      661 tagcatggac agcgatccgg cgaaaatctt tcaattagat gattctcaag ggccggaaaa
      721 aataaaatta ttttcaatgc taaatcatga aaaaggtcta tactatttgg cccgtgattt
      781 gcagtattct attgatcccc ataattacct ttttattctt gtttgcgcaa ataacgcatg
      841 gcaaaacatt cctgccgagc ggctgcgctc atggttggat aaaatgaata aatggagcag
      901 gttaaaccat tgttcgcttt tggtaattaa tcccggaaat aataacgata aacaattttc
      961 attgttgctt gaggaatacc gttcactttt tggtcttgcc agtttgcgtt ttcagggcga
     1021 ccaacatttg ctggatattg ccttctggtg caacgaaaaa ggggtcagcg cccgtcagca
     1081 gcttagcgtt cagcaacaaa atggtatctg gacattagtt caaagcgaag aggcggagat
     1141 ccaaccacgc agcgacgaaa aacgcattct gagtaatgtt gctgtactgg aaggtgcgcc
     1201 gccgctatcg gaacactggc aactgttcaa caataacgaa gtcctgttca atgaagcccg
     1261 taccgctcag gcggcgacgg tggtcttttc tttacagcaa aatgcgcaaa tcgagccact
     1321 ggcccgcagc attcataccc tgcgtcgcca gcgcggtagt gcgatgaaaa tcctcgtgcg
     1381 ggaaaatacc gctagcctac gcgccaccga tgaacgtttg ttattggcct gcggtgcaaa
     1441 tatggttatt ccgtggaatg cgccactctc ccgttgtctg acgatgatcg aaagcgtgca
     1501 agggcagaag tttagtcgct atgtgccgga agatatcact accttgctgt caatgaccca
     1561 gccgctcaaa ctgcgtggtt tccagaagtg ggatgtgttc tgtaatgccg tcaacaacat
     1621 gatgaataac cctctattac ctgcccacgg taaaggcgtt ctggttgccc tacgtccggt
     1681 accgggtatc cgcgttgaac aagccctgac gctgtgtcgc cctaaccgta ccggcgatat
     1741 catgaccatt ggcggtaatc ggctggtgct gtttctctca ttctgtcgga ttaacgatct
     1801 ggataccgcg ttgaatcata ttttcccatt gcctactggc gacattttct caaaccgtat
     1861 ggtctggttt gaagatgatc aaatcagtgc cgagctggtg cagatgcgcc tgcttgcccc
     1921 agaacaatgg ggcatgccgc tgcctttaac gcaaagttct aaaccggtca tcaatgccga
     1981 gcacgatggt cgccactggc gacgaatacc agaacccatg cgactgttag atgatgctgt
     2041 ggagcgctca tcatgatgac catcagcgat atcattgaaa ttattgtcgt ttgcgcactg
     2101 atatttttcc cgctgggcta tctggcgcgg cactctttgc gacgcattcg cgacacctta
     2161 cgtttgttct ttgctaaacc tcgttatgtt aaaccggctg ggacgttacg ccgcacggaa
     2221 aaagccaggg caaccaaaaa atgactcaat ttacgcaaaa taccgccatg ccttcttccc
     2281 tctggcaata ctggcgcggc ctttccggct ggaacttcta ttttctggtt aagttcggcc
     2341 tgttgtgggc gggatatctt aacttccatc cgctcctcaa tttggtgttt gccgcgtttc
     2401 tgctgatgcc cattccgcgc tacagcctgc atcgcttgcg ccactggatt gccctgccga
     2461 tcggctttgc tttgttctgg catgacacct ggttgcctgg cccggaaagc ataatgagcc
     2521 agggttcgca ggtggcgggg ttcagtaccg attatttaat cgaccttgtc acacgcttta
     2581 ttaactggca gatgattggg gccatttttg ttttattagt ggcctggtta ttcctgtcac
     2641 aatggattcg cattaccgtt tttgtggttg ccatactgct atggctgaac gtacttaccc
     2701 tggcgggacc aagtttctcc ttgtggccag ccggacaacc gacgaccact gtaacaacga
     2761 cgggtggtaa cgcagcggca accgttgcgg cgacgggtgg cgcaccggta gtgggtgata
     2821 tgcccgcaca aactgcaccg ccaacaacgg cgaaccttaa cgcctggctg aataatttct
     2881 ataacgcgga ggcgaaacgt aaatcgacct tcccgtcttc gctgcccgct gatgctcagc
     2941 catttgaact actggtgatt aacatctgtt cgctttcctg gtcggatata gaagccgctg
     3001 ggttgatgtc gcatccactg tggtcgcatt tcgatattga gttcaagaac tttaactccg
     3061 ccacctccta cagtggcccg gcggcgatcc gtttactgcg cgccagctgc gggcagactt
     3121 cgcacactaa tctgtatcaa ccggcaaata acgactgcta tctgtttgat aacctgtcga
     3181 aactgggctt tacccagcac ctgatgatgg gacataacgg ccagttcggc ggttttttga
     3241 aagaagttcg cgaaaatggc ggcatgcaga ctgaattgat ggatcaaaca aatctgccgg
     3301 ttattttgct gggctttgat ggttcgccgg tttatgacga taccgccgtg cttaaccgct
     3361 ggctggacgt taccgaaaaa gataaaaaca gccgtagtgc cacgttctac aacacgcttc
     3421 cactgcatga cggcaaccat tatccgggcg tcagcaaaac agcggattac aaagcgcggg
     3481 cgcagaaatt ctttgatgaa ctggacgcct tctttactga actggagaaa tcgggtcgta
     3541 aagtgatggt ggtcgtggtg ccggaacacg gcggcgcgct gaagggcgac agaatgcagg
     3601 tatctggcct acgtgatatc cctagcccgt ctatcaccga cgtccccgtt ggggtgaaat
     3661 tcttcggcat gaaggcacca catcaggggg caccgattgt catcgaccaa ccgagcagct
     3721 tcctggctat ctccgatctg gtggttcgcg ttcttgatgg caagattttc accgaagaca
     3781 atgttgactg gaaaaaactc accagtgggt tgccacaaac agcaccggtc tcagagaact
     3841 caaatgcagt agttattcaa taccaggata aaccgtacgt tcgcctgaac ggcggcgact
     3901 gggtgcctta cccgcagtaa gcacgaaaaa ggccgcagag tttcccccct gcggtctggt
     3961 ccgggcgcaa attgccatta cggcagcctg acgcccgcat gacacgttac ttccgcttgt
     4021 tcagccagtt cacgatcata ctggcaagaa tgccagcaat gaccggagcc gctaaatcat
     4081 gccagaaggc catgcccagc tctgcgagcg tcatatagcc gcctgtgttg taatgacaac
     4141 gtttcgcggc tattcttgag tggtctaggt tcaagattag cccccgtggt gttgtcaggt
     4201 gcatacctgc aacgtgcggg ggttttctct ctccagcaac caatgccacc agggattaag
     4261 cccccgcaac attgcgcctc accggaaccc ttccggcttg ccgctaattt tacgattgct
     4321 ttttactgtc agcactcggc aatcttcctc attgcgtgat gccttccagc ttaatttctt
     4381 aatttgcaat agttgcagtt catatccggg ctgactccag actggtagtg aaatgcgcaa
     4441 ttttgtagaa ttgcgcctcc tcgcgacctc cccagtgaat ctgttgcctc attttttaca
     4501 tactgcgtat tcgacttctc cacctgttgc gcaagagaaa ctgggtttat tcatttttgc
     4561 gaggccgact tctttctgga caggacttta tgcagcacaa cacactaccg aaacacgatc
     4621 agaaattgcc gtttacacgc tacgacttcg gctgggtttt attatgcata ggcatggcga
     4681 ttggtgccgg aaccgtgctg atgccagtac aaattggctt gaagggcatt tgggtattta
     4741 ttaccgcagc gatcattgct tatcctgcca cctgggtagt gcaggacatt tatttaaaaa
     4801 ccctttctga aagcgattcc tgtaatgact acaccgatat tatcagtcat tacctgggga
     4861 agaactgggg aattttcctc ggggttatct actttttgat gattatccac gggattttta
     4921 tctactctct ctccgtggtt ttcgacagcg cctcgtacct gaaaaccttc ggtttaaccg
     4981 atgccgatct ttcacaatct ctactttata aagtcgctat tttcgccgta ctggtagcga
     5041 ttgcgtctgg tggtgaacga ttactgttta agatttctgg gccaatggtg gtggtcaaag
     5101 tagggattat tgtcgtgttc ggttttacga tgatcccgca ctggaatttc gccaatataa
     5161 ccgccttccc gcaagcctcc gtcttttttc gcgatgtcct gcttaccatt ccattttgct
     5221 tcttttctgc agtatttatt caggtactta acccaatgaa tattgcctat cgtaaacggg
     5281 aagcggataa agtactggca acccggctcg cgctgcgtac ccaccgaatt agttatatca
     5341 cgctcatcgc ggtgatactg ttttttgcct tttcgtttac cttctcaatt agccacgaag
     5401 aagccgtttc tgcctttgaa caaaatatct cggcactggc actggccgcg caggtgatcc
     5461 ctgggcatat cattcatatc acctctacgg tgctgaatat ctttgccgta ctgaccgcat
     5521 tctttggcat ttatctcggt ttccacgagg ccattaaagg cattattctc aatctgttaa
     5581 gccgaattat tgataccaag aaaattaact cacgcgtgct gactctggcg atctgcgctt
     5641 ttatcgtcat tacgttgacg atttgggttt cgtttcgtgt atcggtgctg gtgttctttc
     5701 agttgggaag cccgttatat ggcattgtgt cgtgcctcat tccgtttttc ctgatctata
     5761 aagtcgcaca actggaaaaa cttcgcggat ttaaaacctg gctgattctg ctttacggca
     5821 ttttgctatg cttgtcgcca ctgttgaagc tgattgagta aaccggagcg catggccccg
     5881 gttttgtgag ttaacgctgc ggattttcat cctgatcaac agcaaaacaa gctaccagtt
     5941 gaccgccgta gtcttttagc tgcggctgca actgggtgca ggggccgaag cgccgacgac
     6001 agcgggcgtt aaacgcgcag cccggcggtg gattcagcgg gcttggcagt tcaccggtga
     6061 gtttgatgcg ctcgcggcga tcgtccggat tcaggcgcgg cgtcgcggaa agtagcgcct
     6121 gagtgtacgg atggcgcggg ttattgaaga tttggtcttt cgttcccttc tccacgcagc
     6181 ggcccaggta catcaccatc acttcatcag caatgtgctc caccaccgac aggtcgtggg
     6241 agatgaagac ataagaaagc cccagttcct gctgcaaatc catcatcagg ttgagcacct
     6301 gtgcacgcac cgagacgtcc agcgcggaga ccggttcatc ggcaatcacc acgtccgggt
     6361 cgagcatcag acctcgggca atggcgatac gctgacgctg accgccggag aacatatgcg
     6421 gatagcggtc atagtgctcg gttttcaggc cgactttcgc catcatcgac agggcttttt
     6481 cccgacgctg ttctttgctt aagctggtgt tgatcagcag cggctcttca agaatttgcc
     6541 cgattttttt acgcggattc agcgaaccgt aagggttctg gaagacaatc tggattttct
     6601 gccgacgcag cttctgcgcc tgcggatcgt gcttaagcag atcctgcccc tggtaataca
     6661 gctcgccgcc ggtgggcgtt tcaatcatcg tcagcaaccg accgagggtc gatttaccgc
     6721 agccagattc acctactacc gccagcgttt tgccacgttc aaggttaaac gaaacgccgt
     6781 ccagcgcctt caccagacgt tccggagcga acataccttt cttcaccgga taatgttttt
     6841 tcaggtcgat agcctgcaac agcggttgtt gcgaggtggc ctcttgcgta ctcatagtgt
     6901 cggcctcccg gcatcatcaa gtgggtaatg gcatttggac tgacgcccgt cagcgagcat
     6961 attcagcgcc ggttcttcag cgcgacatct gtcagtggca taggggcagc gcgggttaag
     7021 caagcagccg ttcgggcggt cgtacttgcc gggaacaacg ccaggcaacg atgccagacg
     7081 ttctttgtcc tgagcaaatt ccggcagcgc acgcagcaat gcctgagtat acgggtgacg
     7141 cggcgcatgg aagatggcgt gcgcatcgcc cgtttccacc acctgaccgg catacatcac
     7201 gatgatttta tgtgccgctt ccgccaccag cgccaggtca tgggtaatta acaccagcgc
     7261 catgttctct ttctgctgta gctccagcag tagttcaatg atttgcgcct gaatggtcac
     7321 gtccagcgca gtggttggtt catcggcaat cagcagtttt ggccgacagg caatggccat
     7381 cgcaatcatc acgcgctggc tcatgccgcc ggaaagctga tgcgggtaaa catccagacg
     7441 cgaagccgga tcgggaatac cgacctgatt caacaagtca atcgcccgct gacgacgagt
     7501 gctcttatta ccgccctgat gcaccttaat cgcttccata atctggaaac ccacggtgta
     7561 gcacgggtta aggctggtca tcgggtcctg gaagatcatc gccacttcgg cacccaccag
     7621 gttgcggcgc tctttttccg agatacgctg caaatcctga ccgttaaact ccagcttttc
     7681 cgccatcacg cggcccggat aatcaatcag ccccataatc gccagcgaac tgaccgactt
     7741 accggagcca gactcaccca caatcccgac tacttcgccc tgttttacgc tgtagctaat
     7801 gcggtctacg gcgcggaacg gtgcgctttc gtcgccgaaa tgcaccgata atttatctac
     7861 atttaataac gccatctcga acctcttact gcttcagttt gggatcgagc gcgtcacgca
     7921 gaccgtcacc catcaggtta aatgccagca ccgtcagcag gatcgccaga cccgggaagg
     7981 tcacgaccca ccaggcgctt tgcgcgaact gcaacacgtc ggagagcatg gtgccccact
     8041 ccggcgttgg cggctgcgcc cccatgccga ggaaaccaag agcagccata tcgagaatgg
     8101 cgttagagaa accgagcgac gcctgaacaa tcagcggtgc aaggcagttc gggaagatgt
     8161 taataaacat ctggcgcatc gccccggctc ctgccacgcg agaagcggtg acgtaatcgc
     8221 gattcacctc caccagcacg gcggcgcggg ttaagcgcac atagtgcggc aaggcaacaa
     8281 aggtcagcgc cagcgcggcg ttaccaatcg acgggccgaa aatcgccacc agcaccagtg
     8341 ccaacagcag acttggcagc gccagcatga tatcgaccac gcgcataatg atgttatcga
     8401 ccaggccgcc gaagtaaccg gcgatcaggc cgagaataac gcccataatc agcgataaca
     8461 ccaccaccag acagccaacc agcagcgaca agcgcgcgcc gtacatcagc cgcgacagca
     8521 catcacggcc tacatcatcg gtacccagca agtgcgccat gctgccgcct tcctgccagg
     8581 ctggcggggc gagcagtgca tcgcggaact gttccgccgg gttataggga gcaatccagt
     8641 tggcaaagat cgcgataaac agcacgatga cgacgtaaac cagcccgacg accgcgcctt
     8701 tgttgcgttt aaaatagtgc cagaactcct gtaaaggggt catcggtacc ggcgcgctaa
     8761 tcactttatt ttcagtaacc tgtgacatga tgacccctta cttcttatga cgaatacgcg
     8821 ggttcaccac gccatacagc agatcgacca gcaggttgac gaggataatc atcgtcgcca
     8881 ccagcaatac gccgccctgc accaccggat aatcgcgccg ttgcagtgcg tcaatcaacc
     8941 agcgccccag accgggccac gagaagatgg tttcggtcag aatcgccccc gccagcaatg
     9001 ttcccacctg gaggccgata acggtcacta ccggcagcat cgcgttacgc agcgcatgga
     9061 cgataatcac ccgcatgcgg gtcaaccctt tggcgcgcgc ggtgcggatg taatcttcac
     9121 ccagcacttc cagcatcgag gagcgtgtca tacgcacaat gaccgccagc ggaatagtac
     9181 ccagcacaat ggcgggcaag atcatatggg cgacggcatc gataaagtta ccgtcttcac
     9241 cccagatggc ggtgtcgatc agcataaaac cggttaacgg attggaatca tcgaggaaca
     9301 ccatatcgct cacgcgaccg gagacgggtg tcaggttcca gtgcaccgaa accagcatga
     9361 tcagcatcat gccccaccag aagataggca ttgaataacc tgtcagcgcc aggccaaccg
     9421 ctgtgtgatc gaaaatggaa ccgcgtttaa ccgcggccag cacaccaacc ggaatgccga
     9481 ccgcggtcgc aaaaatcatc gcgcagacgc caagttccag cgtggcctga aagcgcggca
     9541 cgaactcttc ccataccgga attcggcttt tcattgaaat gcctagatcg ccatgcataa
     9601 cgccccaaat gtaatggaga tactgctgcc acatcggttt atctaagccg agttcagcca
     9661 gcaactgcgc gtgacgctct ggggagatcc cacgttcgcc cgccatgatc atcacaggat
     9721 cgcccgggat catgtggaca aaggcaaatg tgagaagggt aataccgata aacgtgggga
     9781 tgacgagtcc caaacgtcgg agaataaact gcaacataac ccggattctc tgtagtgacc
     9841 cgcagcctgg aactgcgtgt ctgtattgct cacaaatcaa ttcccgccca ggtcgggcag
     9901 ggaatgaagt attgctgccg gatggcgctg cacgcagagg cacagcacca ccgggtatgg
     9961 cttttaatta ttcgatagag acgttttcga agtgatgttt gcctaatgga tcaaccacat
    10021 agcctttaac ttctttgcgt accggttcaa acacggtgga gtgagcgatg atcagtgccg
    10081 gagcctgatc gtgcatcacc acctgcgctt gcttgtacag ttcaacgcgt ttgttatggt
    10141 cgtcggtagc acgcgccggt tgaatcagat cttcaaacgg tttgtagcac cattttgagt
    10201 agttggagcc ttgttcagag gcggcgcagc tgaacagagt ggcgaagaag ttatccggat
    10261 ccccgttatc gccagtccag cccatcatta ccgtctggtg ctcgccatct ttcgcacgct
    10321 tgaggtactc accccattcg taggtgacaa ttttggcctg cacgccgact ttcgcccagt
    10381 ctgcctgaat catctccgcc atgcggcgag cgttcgggtt atacggacgt tgtaccggca
    10441 tcgcccacag gtcgatggag aaaccttttt ccagacccgc ttctttcagc aaggctttcg
    10501 ctttttcagg atcgtaggtg tagtcctgaa cgtcgtcgtt atagccccac atggttggcg
    10561 ggatcaggtt tttcgctgat acacccgcgc cctgataaac cgctttgatg atcgcgtctt
    10621 tgttcaccgc gtaggtcaga gcctggcgaa ctttcacgtc atcgagtggt tttttctgca
    10681 cgttatacga gagataaccg acgttcagcc ccggcatttc catcaggttg atagatttat
    10741 cctgcttcat gcgggcgata tctgccgggt tcgggtacgg catcacctgg cattcatttt
    10801 tctgcaattt agcgtaacgc acggaagcgt caggggtaat agagaaaacc agcgtatcga
    10861 tctgcggttt ggtgccccag tagccatcaa acgctttgta gcggatacgg gaatcttttt
    10921 gatactgctg taactggaac ggaccggtgc cgattgggtt gaggtccagt ttttccggtg
    10981 taccggcttt catcatcgca tcagcatatt cttttgacag aatagaggcg aagtccattg
    11041 ccaggtcagc gaggaacggc gcttccgggc gagtcagcac aaactgaacg gtgttgtcgt
    11101 ccaccttttt cacttcgctg atcagctccg gcaagcccat gccttcgaag tattcgtagc
    11161 tgccgccaga aactttatgg tacgggtttt gcgcgttttt ctgacgatcg aacgagaaca
    11221 ccacgtcatc ggcattcatt tcgcgcgtcg gtttgaattc tttattgtcg tgccacttca
    11281 cacctttacg cagatggaag gtataggttt taccgtcttc gctgacttcc cacttttcag
    11341 cgaggcccgg aatcacttcg gtggtgccga ttttaaattc aactaggcgg ttataaagcg
    11401 gtacggaaga ggcgtcatag gtggtaccgg aggtaaacag ctgcgggtta aacccttccg
    11461 gagatccttc tgagcaataa accagagttt tagcctgaac acttgctgcg acggtcatag
    11521 ccaccaggct gagaccaagc ttcagcatcc ctgacttttt caaggaaata cgcattattc
    11581 tgctccaatt gtgatgtttg ttgttttaac cctttgcagt gggttgtcgc tgcctgacct
    11641 tttttgtttt ttgcccggtc gggtcaacgt tatgaggtgg ggatgccgta ctaattaaca
    11701 tcagtgtaac agtgcggatc ctccataaaa tgcccctcgt gacctacaat ctgtcaacag
    11761 aatgtgaaaa cgtcaataca gccaaccggg atttacacca acggtgagaa tccacaaaca
    11821 aagattaaaa aaacttcaaa cagctatttg cagcagagaa atttgtgcta ctccaacatg
    11881 accagaacaa tcagcttaat atttagcaac atttggtgat taagttttat gcatttagtg
    11941 aaaaaaatct gaggaaaagg tggatatctg agcgattaat accatgaacg ctaaaacgca
    12001 cagcggaaaa tgccagcgcc agccataagt aacgcaaaaa aagatttaag caaatataaa
    12061 aaaagacaat ggtttctgtc acaaatccgt taatggatag tgagatatgg ggcgcaaatt
    12121 tggcagggaa atgtgtggcg tgtaggtcga ataaggcgtt cacgccgcat ccgacaataa
    12181 gtgcggtgcc gttgcctgat gcgacgctga cgcgtcttat caggcctacg tcgagtcgaa
    12241 cctccctgcc cccggaggtt ctcttcctca tgggctgcag atgcaaaaaa gcctgctcgt
    12301 tgagcaggct tttcgaattt ggtcggtgat agaggattcg aacctccgac cccttcgtcc
    12361 cgaacgaagt gcgctaccag gctgcgccaa tcaccgaatg cggggcgcat cttactgcgc
    12421 agatacgccc tcgtcaatcc cttaatagca aaatgccttt tgatcggcga gaaagtcagc
    12481 aggcctctta gttagccgct gcctcttttg tctgcgggat gtgacaccag ttgttgtttt
    12541 cgttaattcc accatccggt gaagtatagc caagacaacc catgatggtg tcgtacagct
    12601 ctacgtgacg gcgtggcact ttcatgtcgg ctgctttttt cagctgcgca aacgcctgcg
    12661 cattgaccgg attttccaga tatttatctg acatccagac catcatcggt acgcggaact
    12721 gctccggcgg tgccagttca cgcggcgtgc cgtgcaggtg ctcgcgttca ttaattgact
    12781 cgccgtggtc agctgcgtag aacacaatcg ctttcttatc gcgaacctga tcaatcacac
    12841 tggagataaa gtgatccaca taggtcaccg agttgtcata ggagttgatc atctgcgctt
    12901 tggtacatcc gctatcaaca ccaatacatt ccggcttcca ctgcgcaaag ctgcgcgggt
    12961 aacgctgggt gtagttaaag tgcgaacctt tggtatgcag aatgattaga tgcttaccat
    13021 ccgggttgcg ccccagcgat tgctgcattt cgtctaccag caacatatca tctaccggct
    13081 tgccacggtt acgtggctcc gcaccaatct gttcacggta agcaatattg tccgccatcg
    13141 tattgctgta gaaccacatt tcgctctgca tagcgtagag gtcagaactg aatcctaact
    13201 gcttcagtac cgcgaaaatg ttctgttctt ttaatgtgcg ctgcggatta tcttccgcgc
    13261 ccccctgacg tacaaacatg caacgcagag agagtttggt tgcggtatca caggagtaac
    13321 cacggaacgc cgccagattt ttctcctggg ccagcttcgg cgtggtatta cgctcatagc
    13381 cgaaaatgcc catatggtcc caacgcgtgg tttcaccgat gataaacacg acataagtat
    13441 cattaacatt ttgcggtgcc tgataagtga atttcttcgc cgggttaagc aatgaattat
    13501 tatcggaaga ttcatcgacc cgcgcccagg cgtacagccc caacgcagaa agccagtttg
    13561 atggcagata agagttcgct acgacaccgc cataactcgg caaatcaacg ccggtcgccc
    13621 tctccacttt cttctgctgg atatccagca aacgaatcgg tgcccaaacc ataattcccg
    13681 ccagtacgac gaccgccagg ctgcgaatac gctgccctgg ggttcgcagt tgtcggagca
    13741 aggtgtagcg acagcggtta ttccagataa ggataagagg caatgcgcta acggcgatta
    13801 accagagaat aaagttcaga ccaacaactt cttttgacag gtcgatatcg gtggtcatga
    13861 cggaagcgat gatgccataa ccaatgacca cattaaggaa ggtcatgtaa tagctggcac
    13921 ctgcggaaaa gagcacaacc agcgatgcca gaatacgcca gctgcggcgg ccaaacagcg
    13981 aaagaagacg tagtaaaaag aaggtcacca gtacggtggc ggccagttca acaacagcag
    14041 aaatgccttt ccagacggta aaatcgtgcg catagctgcc gaagcggcgg taaaaaaccg
    14101 cgccattcat aaaaaggcca atatagattg caagcaaaaa gctcagcttc tgctgtgtaa
    14161 tcgatttgat gtatctcatg caaacaaacc ctggacaaac aggtgataac accctgctta
    14221 gcggcttttc aggcccagca ggggaaaagt gcggaggtag tcagataagt aaaatctaca
    14281 cgcgcgaata agtagaccac agcaaaggga aaaagtgtgg ggaaagagtg tgcatgaagc
    14341 acaaatttac aaaagtgcaa gtccggaata gaccatttta acggattaac gactattcag
    14401 agataggttg ggccaggatg ctggcccagt aacttactca taggtgatgg aaaaattgac
    14461 aagggcattc gctgtgccac cagtggcctg agtgttgacg accacataac tcgcctggta
    14521 gtgaagcgac gtggtttcgc catcaatcgc ccattcaatc ggcgttccgc tggagccttt
    14581 gggaacttgc tgcgttcctt tatcgtcctc tatcttcaac gcaacacccg ttgcgccacc
    14641 agcctcaagg gcaaacatgg aatcatcatc gctgtccgac tgactgtcca tctcaacggt
    14701 cgctttggat atcccatcgc actctttgag ttcaatggtg aaatctttct tgctcaacac
    14761 cgtaccgact gccgcaggtt tttgcagcgt cattgttccc atatcgactt cgagatcgct
    14821 tttttccatc tcgcaagctt tagtggtgat agtgatggag aaatgaatat cccctgccgg
    14881 gccaagatcc gtcgccgcat tcaccccgca ggcaaccaga ctcaaactgg cgatgccgct
    14941 atgcagcaat cgtttgaact tcatggcatc actccatttc aagatatatg gtcgttacgc
    15001 cttcaaacgt cccgccttta ggcgctttac ccgtcgtgct aatcggtaaa gccagcagtc
    15061 gaatcgcggc attgcggtga gttttagaat ccaggctgct caaatttaat aacgtggtgc
    15121 tgcttgaatc attcggtacc aaaattttgc cgctggaatc tttcaccagt acgccaatat
    15181 cggggttgcc catatccacc gctcggggat cgtcagcatt ggttgccccc tcaaggcgca
    15241 acgcaacctt gataccatca gagatattgt tgcaatcgaa gctgaggtta atttcttgct
    15301 ctgtcgcacc ttcaggcatt tgcccttgcc tgtttttaaa cgccgaggag gggaattcac
    15361 caaatggcac atccagcacc gtgccgggtt taatttcgca atcctgggtt acggtgacac
    15421 gcccctgaat agatacggaa gatatgggta ttgttccgta atctttattt gttttcgagg
    15481 catacagttc ggcaattaac gtcagaggga tgacggaatc gccgataaag ggtttagtaa
    15541 tagcaaagga aagtagacct gcgccgccca tatagggcgt agtaatgact ttgttgcatc
    15601 cggttgtttg cagcgcctgc tggtttttga aaggaacaaa cccatacaca ctggtactga
    15661 tgcgaatgtt cgtcgcaacc tccagttcct ccgtcaaagt ataatatttc atactgtcaa
    15721 ccgtacggcc agaaggggga agcatactca ccccctgcac tagagtatct ttttcattct
    15781 tataagaatc aggacattca caaattagcg atacgttttg tccattagac cagttaagga
    15841 catccggtaa aaccgttcct gcgacgtttt ttgccgcatc aggtatacca acatccacat
    15901 cgacattata gtgataagtc gtgccatccg cggggggaca tggcccccac gcatctccgg
    15961 caaatgcctt gccagaaaat ccgaatacgc atcctaataa ggataatgct attgccgctt
    16021 tcatttcgct ctccttgctt actggcaatt ggccgaaaca ggaataattc cgccctgttt
    16081 taattccagg gataatttat aaggcgccac gcattgctgc gacgcgtcat taccccattt
    16141 aatcaatacc cgtccttcct ctggcattcc gctaatatag agctgtcctt cttcaccaac
    16201 gatactgctc acctcgtttt gtttatcggt gattagcgtt gccgttgctc caaagggcac
    16261 cgggctgccc gcggaacgaa gcagcgtcat taatacgcgg taaccaacgc gggtatcaaa
    16321 acgcgctctg acaatcgctc cacgcgttgg caccactttc tggaacgcat tttcaacgtc
    16381 aacatcgtca cccaacgaat cgctacgcag tgagatttcg ttttcacgat acggcatggc
    16441 ataaggcacg atcgcgttac ccatccagtc aacctgaacg ccgccgttat tcaccacgcg
    16501 ggcattgcgc gcaccgggta cggagatgag cgtcatggtt tcgcctagcg gttgggaaag
    16561 cgtcacgcct tcgctatgaa ctatcacgcc gccgcgaacg ctgtagttga gctgccggta
    16621 atctttcccg tagctatagc ctacgttgac attaccggac ccaccctgat agccaacgtt
    16681 agcgttaccg ctgttaccca caccgttgct ggcgtagcct tcctggacac tgtagctcag
    16741 gttgcgatcc tcaagcagcg ttccgctgac cccaacctgt tgattggtac gaccatcctg
    16801 gtcggtcgtg acgcgatagt tactccaggc ccggcctagt ggaacggaaa tattgaaaga
    16861 ccacaagcga tcgctttcat cccattcagg gcttttattc cagctatatg caatactgta
    16921 actgaccttg ccaatacgtc cgttgtaacc cgccgatacc gtgttctgtt tacctgcgtc
    16981 gttccagtaa tcctgctgcg ttaaatttaa ataaacagag ccataggccc ctaattgctg
    17041 cgtcaggtta ccctgtattt cgctgcgctt gtgataacgg ttatagtcgc tgtcagcgtc
    17101 actgcgcaca tcggtggctt cctggaaggt atagaaaccg ctggtagagt accgatagcc
    17161 cgcaaggcgg aaatcggtgc cgctttcgaa gctcttggaa tataagaaac gataagattg
    17221 tccctggcta tcgcgatcgt tattcagttc gcttttagcc tgcgtcacat caatggaaat
    17281 cgccccgata taaccaaagt ttttccctat ccctaatgta aatgcattgt aattattaga
    17341 gattaatacg ccgccgtagg ccgtcatccc ccacggtaaa ccgtagatca gatcaagctg
    17401 cccgaaacgc ggcgaggcgc tgtcataatt cccggcctga tattcacctg cggcaaagct
    17461 atatttgaga tggccttcac gctggaaaat tggcaccgat gagaatggct aaataaagcg
    17521 ttgttcactg ccatccgctt ctttaataat gaccgtgaga tcaccgctgt ttgcagtggg
    17581 gtaaagatcg tttatttcaa aggcaccagg ctgcacatag gtgcgataaa taacgtagcc
    17641 gttttgttca atactgactt cggcgttgct tttggcgatc cctcgaacga tgggtgcaaa
    17701 gcctcgctgg ctgtcgggca gcatctcttc atcagaggct aacatcgcgc cacgcatctg
    17761 aacgctgtca aaaatgtccc ccgcggtcgc cgtatccccc attgttaatt gcgctttgaa
    17821 agggatgatg gccctactca aagacgtacc gatattatcc cactgcgctt tgccaccgga
    17881 atggctccag gtactgtaat tccgtaaccg ccaagcgcca aggttcagcc cgctgcgtaa
    17941 gttgaggtag tagctgtcgc tctttagcgt atctttacca tcatcatgat gaacagtgcc
    18001 attatcatcg acataactgc tggaagaccc cgtcgagtca tattcactgt tgctcccgct
    18061 aaaactgtaa tccagcagca acgcagggat accttcgtcc cataaagatt ccggcaccgt
    18121 gccgcgcgcc acctggtgca tcgccgcctg agggaaactt aataccagct tttgttcact
    18181 gaagtcgaaa tggcttgagg ccaggggaat gatttcgtca aacggtacgc aggcttcggc
    18241 agacaccaga ttgagggccg gaaagctttc aacgcgcacg cccatctctg ccagttgcgt
    18301 ccggctaagg caaggcacaa gggattcccc catcgctttg cgctgtgctt ctgtggcctc
    18361 tttaaattcc agtgtgcggg tttccattct ctgttcattg acgtaaacag agacacgata
    18421 gatgccgggt aactgctgtt cctggccttt ttcgaacatc gttaaatcga catgctggcc
    18481 cacaccctga acgtcctcca ggaatttggg gttaaacttt ccggcatgtg cagaaaacgt
    18541 gctggcaaca gcaaccgcca ccacagagat agaaaaggaa gaaaaagtac gtgaaacggt
    18601 cttccgtgac attgagctat ttctcctgaa
//
LOCUS       DAZKFZ010000075        18560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000075 DAZKFZ010000000
VERSION     DAZKFZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..580)
                     /gene="rsmG"
                     /locus_tag="ACHOQW_002414"
     CDS             complement(<1..580)
                     /gene="rsmG"
                     /locus_tag="ACHOQW_002414"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9573483.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPAL"
     gene            complement(644..2533)
                     /gene="mnmG"
                     /locus_tag="ACHOQW_002415"
     CDS             complement(644..2533)
                     /gene="mnmG"
                     /locus_tag="ACHOQW_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9573484.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2912..3355)
                     /gene="mioC"
                     /locus_tag="ACHOQW_002416"
     CDS             complement(2912..3355)
                     /gene="mioC"
                     /locus_tag="ACHOQW_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP9573485.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3445..3903)
                     /gene="asnC"
                     /locus_tag="ACHOQW_002417"
     CDS             complement(3445..3903)
                     /gene="asnC"
                     /locus_tag="ACHOQW_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP9573486.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4055..5047
                     /gene="asnA"
                     /locus_tag="ACHOQW_002418"
     CDS             4055..5047
                     /gene="asnA"
                     /locus_tag="ACHOQW_002418"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP9573487.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5052..6503)
                     /gene="viaA"
                     /locus_tag="ACHOQW_002419"
     CDS             complement(5052..6503)
                     /gene="viaA"
                     /locus_tag="ACHOQW_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP9573488.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6497..7993)
                     /gene="ravA"
                     /locus_tag="ACHOQW_002420"
     CDS             complement(6497..7993)
                     /gene="ravA"
                     /locus_tag="ACHOQW_002420"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP9573489.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8216..10084
                     /gene="kup"
                     /locus_tag="ACHOQW_002421"
     CDS             8216..10084
                     /gene="kup"
                     /locus_tag="ACHOQW_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP9573490.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10251..10670
                     /gene="rbsD"
                     /locus_tag="ACHOQW_002422"
     CDS             10251..10670
                     /gene="rbsD"
                     /locus_tag="ACHOQW_002422"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9573491.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10678..12183
                     /gene="rbsA"
                     /locus_tag="ACHOQW_002423"
     CDS             10678..12183
                     /gene="rbsA"
                     /locus_tag="ACHOQW_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP9573492.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12188..13153
                     /gene="rbsC"
                     /locus_tag="ACHOQW_002424"
     CDS             12188..13153
                     /gene="rbsC"
                     /locus_tag="ACHOQW_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9573493.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13178..14068
                     /gene="rbsB"
                     /locus_tag="ACHOQW_002425"
     CDS             13178..14068
                     /gene="rbsB"
                     /locus_tag="ACHOQW_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP9573494.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14194..15123
                     /gene="rbsK"
                     /locus_tag="ACHOQW_002426"
     CDS             14194..15123
                     /gene="rbsK"
                     /locus_tag="ACHOQW_002426"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9573495.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15127..16119
                     /gene="rbsR"
                     /locus_tag="ACHOQW_002427"
     CDS             15127..16119
                     /gene="rbsR"
                     /locus_tag="ACHOQW_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP9573496.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16085..17512)
                     /gene="mdtD"
                     /locus_tag="ACHOQW_002428"
     CDS             complement(16085..17512)
                     /gene="mdtD"
                     /locus_tag="ACHOQW_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP9573497.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(17535..18227)
                     /gene="yieP"
                     /locus_tag="ACHOQW_002429"
     CDS             complement(17535..18227)
                     /gene="yieP"
                     /locus_tag="ACHOQW_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9573498.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4470 a         4603 c         4860 g         4627 t
ORIGIN      
        1 ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg ggcaacaaag
       61 cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc tcaccaggaa
      121 gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta attacgccat
      181 caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca atattctcca
      241 gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg ctatccaaca
      301 gagtgaaatg ggcttcagga cgcacgatag agagtggaat gccaggcagt ccaggaccgg
      361 tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg ctatcgagaa
      421 tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac gctttgttcc
      481 atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg gtaagcgaaa
      541 tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc tgttcttgat
      601 gcgttgcctg gtaagcgggg gcttaccagg catttttaat gcgttatgcg ctacgacgca
      661 gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc gtgacgccag
      721 aaatacgtga agcctggccg atagaggccg gtttgtgatc gttaagtttg gcgatcactt
      781 cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcaag gtgttctcgt
      841 tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg tatttaacct
      901 gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac ggcgtcagcg
      961 tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca ctggcttcac
     1021 gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc gacggggtta
     1081 cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc tcgttaaagc
     1141 gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc aaacgcagat
     1201 ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaggtaaac atacgatacg
     1261 gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatag gcctgagaac
     1321 gcgccggagc ccaaccttct ttgtcatcag acagacgggc agcgttaaga ccggccagca
     1381 aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca gcaaagaaca
     1441 gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg aagaagtcat
     1501 actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc atagagcgga
     1561 cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga taaatttcgt
     1621 tagaagtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg gcgaagcgca
     1681 tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc acccctgcgt
     1741 acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg ttggtatggg
     1801 tgatataaca cggcacctgc tgtggatgct gggacgcatt gcccataaaa gagaataccg
     1861 gcattgggtt atcgccatgc tgttgcgcaa gcacgctaaa gtcgatggtt cgagcatcaa
     1921 tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggaagttca cgcaaacggc
     1981 gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtagtta tccagaccga
     2041 tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg gcacggaact
     2101 tcagtcccat ttgggtaaca gcaccgacca cacgatcgtt ttcgacaata agatcttcaa
     2161 ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt accgcctgac
     2221 ggtagagcac acgatccgcc tgagctcggg tagcgcgaac tgccggtccc ttgcttgcgt
     2281 ttagtatcct aaactggata cccgcctgat cgatcgcttt cgccatcaga ccgccgagtg
     2341 catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg ttgcagctca
     2401 tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc atacgcgccg
     2461 cagccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg tcaaaaggat
     2521 ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt gaagcccggg
     2581 ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg gtattaaaaa
     2641 gaagatctat ttatttagag atctgttcta ttgttatctc ttattaggat cgcactgccc
     2701 tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca ttaactgtga
     2761 atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat acacaactca
     2821 aaaactgaac aacggttgtt ctttggataa ctaccggttg atccaagctt cctgacagag
     2881 ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc cacgatccca
     2941 gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc ttcagtgttt
     3001 cgcccgtctg ttttgcaccg gaatttttga gttcagcctc gagtttatcg atagccccac
     3061 aaaatgtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc gcagaaagat
     3121 cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga atatctccgg
     3181 caccgtgggt ggagctgata accagccaga tccccgaggc agataaatct tccaacagcg
     3241 gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc aggtgttctg
     3301 ctacatattc ggcaccgccg agggtgctgc cgctgataag agttatatct gccataaacc
     3361 gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc tacctgtgga
     3421 aaagtatggg attaaaaaag ccgatcaggg cttgatggta cgcatgatcg ggttctgcag
     3481 gacgatcagt gtctcggtgg actgaatttc atcaattgtt tggatcttgt tgataagtac
     3541 atgctggaga gcatcgatcg aacggcacat cacttttata aagatgctgt agtggccggt
     3601 ggtgtagtac gcctcggtca cttcatcgag gctttccagc tttgccagcg cggaagggta
     3661 gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac cgagctgctt
     3721 cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt tctctactcg
     3781 aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt aagcggtgcg
     3841 cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca gataattttc
     3901 cataggattt tcttatgcgg attgatgatt cattctattt tagccttctt ttttaatgaa
     3961 tcaaaagtga gttaggcttt ttattgaatg attattgcat gtgtgtcggt ttttgttgct
     4021 taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca ttgccaaaca
     4081 acgccaaatt agcttcgtga aatctcactt ttctcgtcaa ctggaagaac gtctggggct
     4141 gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg ataacttgtc
     4201 gggctgtgaa aaagcggtac aggtaaaagt gaaagctctg cctgatgccc agttcgaagt
     4261 ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact tcagcgcggg
     4321 cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc tttctccgtt
     4381 gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg gtgagcgtca
     4441 attctcgact ctgaaaagca cggtagaggc gatttgggcg ggaattaaag caaccgaagc
     4501 tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc acttcgtaca
     4561 cagccaggag ttactgtctc gttatccgga tcttgatgcc aaagggcgtg agcgggcgat
     4621 agcgaaagat cttggtgcgg tattccttgt cgggattggc ggcaagctga gcgatggtca
     4681 tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag agctgggcca
     4741 tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg cgtttgagct
     4801 ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg cgctgaccgg
     4861 tgatgaagat cgtctgcagc tggaatggca tcaggcgttg ctgcgcggtg aaatgccgca
     4921 gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc aactgccgca
     4981 tatcggccag gtccagtgtg gagtatggcc agctgctgtt cgcgagagcg tcccttctct
     5041 gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc ggtatcaaag
     5101 cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga catcgccacg
     5161 gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt cacgtcgtca
     5221 ggcaaccgct gggcgataaa atcagaaatc accaccgcat cggcatcaaa ccattccctg
     5281 ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc gccacgaaac
     5341 tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag ctcataacgg
     5401 acgatctcgg tggaaaatag cataatataa cagcgccggt tttcagcgag agcaatgcgc
     5461 atcaaagcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat tgagccggaa
     5521 gtatccacgc agacaataaa cggtccgcgc ggctgttcgt cgtaatcttt atgcaccacc
     5581 gggcgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt gagcaactgt
     5641 ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag tgtcgccagt
     5701 tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac ctgctcagga
     5761 accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc gttgcgcggt
     5821 attgatttgg cttcccggga acgccccagt tgttctgcca ggcgtttcag ttccggctgt
     5881 tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt aagctgaccg
     5941 gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat cggttcaagt
     6001 tgtccgctca gcgtcatgcg ttcctgaact tcactcaaca gttgttcgcg ttcttcttct
     6061 aatagctgtt gatttaacgt tgttgcttgc acgatcagac ttaaacgcca gcgctggaga
     6121 aaaagcgtgt gtaacgctga agtgatcgtg ctgttagcat cgaccaactg tcgggcttgt
     6181 tctgcccatg gggaattcag acgatgcagt aagtctagga tctgtggtag ctgcacaata
     6241 aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc ggtgagttcc
     6301 ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg gggaacatca
     6361 tcagtgattg ccgccttcag tcgtgggaat ttttcaaaga agactgccag ctgcggtgag
     6421 gccagcagcg cgatgatcat ctcttcgatc aatccctctt cgctgacggc cagcatcaca
     6481 ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcgccg acatcctgta
     6541 ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatctt tgctgttcgc
     6601 tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccaattgt tgttttattt
     6661 cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct tgtaaactta
     6721 catcgcgtac aacaagatgt tgggcgctat caacttccag attcagtttc tgggcaaagc
     6781 cgataccgtt cagtttgccg cgaatttcac cacctttgct cagccactgt tccagcgcgc
     6841 tacgctcaaa ggagatatga accacttcca tattatgcaa ttttaacggt ttttgcagca
     6901 gcagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga cgacggctga
     6961 aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc tgctggagtt
     7021 gcaggtgacg ttgcacaatc gcgcccaggc gggtcaacat cccttgctgt tgccaggcat
     7081 gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc gcgtcatacc
     7141 acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga ccgctaaaaa
     7201 aggcgctggc ctgcaataat cgaatcgctt ttttccagcg acgatccgag acataaggcg
     7261 catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat acatgatcgg
     7321 gcagcgtaat ttcaccaatc tctttttgcc agcgttcata ttcttcatct gtgacctgca
     7381 aggaggctgg aaccggattg tcattttcat cctgttgact ggtcagcatg gagcggaaat
     7441 tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca tataacgctt
     7501 ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcagg cgcatcggga
     7561 ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg agcagggtat
     7621 taagaattgc cggacccgct ttccagatct catccagaaa gacgatttct gcttccggca
     7681 ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga atagaaaggg
     7741 gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac gcgcgggcat
     7801 tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct ggcgggccaa
     7861 ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg gcgtggctac
     7921 gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc gctaataaat
     7981 gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa attgcgagta
     8041 tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gggtattttt ctttgagcca
     8101 ggttaatgtg gccgcattta ggagtacgat tttgccgtta atcgtgcata ctgtgcgctt
     8161 ttttgtgggc caagggacta agcacacatt tcatatttca acgaaagact agtctatgag
     8221 cactgataat aagcaatcat tgcccgcgat taccctcgcg gcgattggag ttgtctacgg
     8281 cgatattggt accagcccgt tatatacact tcgtgaatgt ttgtccggcc agtttggttt
     8341 tggcgttgaa cgcgatgccg tgtttggctt tttatcgctg atcttctggc tgctaatctt
     8401 tgtggtttcc attaaatatc tcaccttcgt gatgcgggca gataacgccg gtgaaggggg
     8461 gatcctgacg ttgatgtcgc ttgccgggcg taatacgtcg gcgcgaacca catcaatgct
     8521 ggtgattatg gggctaatcg gcggcagctt tttctatggt gaagtcgtca taacacccgc
     8581 tatttcggtg atgtcagcca ttgaaggtct ggaaatcgtc gccccgcagc tggatacctg
     8641 gatagttccc ctctcaatta tcgttctcac tttattattt atgattcaaa agcatggcac
     8701 ggctatggtc ggtaagctgt ttgcgccgat catgctgacc tggtttttga ttctggcagg
     8761 actggggtta cgtagcatta ttgctaacca ggaagtgctg tatgcgctga atccgatgtg
     8821 ggcggtgcat ttcttccttg aatacaaaac ggtttctttt attgcattag gggcagtggt
     8881 gctgtcgatt acgggggtcg aggcgctgta tgctgatatg gggcactttg gtaagttccc
     8941 tattcgcctg gcgtggttca ccgtcgtatt accttccttg acccttaatt acttcggtca
     9001 gggagcgctg ttgttaaaga atccggaagc gattaagaac ccgttcttcc ttttggcacc
     9061 ggactgggcg ctgatcccgc tgctgatcat cgccgcactg gcgacggtaa tcgcctcgca
     9121 ggcggttatc tctggcgtct tttcattgac gcgtcaggcg gtacgtctgg gatatttgtc
     9181 gccgatgcgc attattcaca cctccgaaat ggagtcaggg caaatctata ttccgtttgt
     9241 gaactggatg ctctatgtcg cggtcgtgat tgtgattgtc agctttgagc actccagcaa
     9301 cctggcggcg gcgtacggga ttgcggtgac cggaaccatg gtgctgacgt ctattctctc
     9361 gactaccgtg gcacgtcaga actggcactg gaataagtat tttgttgcgc tgatcttgat
     9421 tgctttcctt tgtgtcgata ttccattgtt caccgctaac ctcgataaac tgctctccgg
     9481 cggctggttg ccattgagcc tcggtacggt gatgtttatc gtgatgacca cctggaagag
     9541 tgagcgtttc cgcttgctgc ggcggatgca tgaacatggt aactctctgg aagcgatgat
     9601 tgcttcgctg gagaaatcac cgcctgttcg cgtgcccggg accgcggtat atatgtcgcg
     9661 tgcaatcaac gtcattccct ttgcgctgat gcataacctt aaacataaca aggtattgca
     9721 tgagcgggtg attctgttaa ctctgcgcac cgaagacgct ccatatgtcc ataacgtccg
     9781 tcgggtacag attgaacaac tgtcgcccac tttctggcgc gtggtggcaa gttatggttg
     9841 gcgagaaacg ccaaacgtgg aagaagtttt ccaccgctgc ggtctggaag gattaagttg
     9901 ccggatgatg gaaacctcct tctttatgtc gcatgagtcg ttgatcctcg gcaaacgccc
     9961 gtggtatttg cgtctgcgcg gcaagctgta cttgctgctg caacgtaatg cgctgcgtgc
    10021 accagatcaa tttgaaatcc cgccaaacag ggttatcgaa ctgggtactc aggtcgaaat
    10081 ctaacgccag acgcctcctt tcttcataag ggggcgtttt tgttttcatg gttaatcacc
    10141 atgtaaaacg tttcgaggtt gatcacattt ccgtaacgtc acgatggttt tcccaactca
    10201 gtcaggatta aactgcgggt cagcgaaacg tttcgctgat ggagaaaaaa atgaaaaaag
    10261 gcaccgttct taattctgat atttcatcgg tgatctcccg tctgggacat accgatacgc
    10321 tggtggtgtg tgatgctggt ttacccatcc ccaaaagtac aacgcgtatc gatatggcat
    10381 taacccaggg tgtaccttct tttatgcagg tgctgggcgt cgtcacaaat gaaatgcagg
    10441 tcgaggctgc cattatcgcg gaagagatca aacaccataa tccgcaactc cacgaaacgt
    10501 tgctcactca ccttgagcag ctgcaaaaac accagggaaa taccattgaa attcgttaca
    10561 ccacgcatga acaattcaaa caacaaaccg cagaaagtca ggcggtaatt cgcagcggag
    10621 aatgttctcc gtatgcgaat atcattctct gtgctggcgt gacgttctga ggccgtcatg
    10681 gaagcattac ttcagcttaa aggcatcgat aaagccttcc cgggcgtaaa agccctctcg
    10741 ggcgcagcgt taaatgtcta tccgggccgc gtgatggcgc tggtgggcga aaacggcgcg
    10801 ggtaaatcca ccatgatgaa agtgcttact ggcatctata ctcgcgatgc cggtacgctt
    10861 ttatggctgg ggaaagaaac gacatttacc gggccgaaat cttcccagga agccgggatt
    10921 gggattatcc atcaggaact gaacctgatc ccgcagttaa ccattgccga aaacattttc
    10981 ctcggtcgtg agtttgttaa tcgctttggc aaaattgact ggaaaaccat gtatgccgaa
    11041 gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagct ggtgggcgat
    11101 ctttccatcg gtgaccagca aatggttgaa atagccaaag tgctgagctt tgagtcgaaa
    11161 gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga atccctgttc
    11221 cgcgtcatcc gcgagctgaa atcgcaaggc cgcggtattg tctatatctc ccaccgcatg
    11281 aaagaaatct tcgagagttg cgatgacgtt accgtttttc gtgacggaca atttattgct
    11341 gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt gggtcgcaag
    11401 ctggaagatc aatatccgca cctggacaaa gcgccgggag atatccgcct gaaagtcgat
    11461 aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg cgaaattctt
    11521 ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct ctacggcgca
    11581 ctaccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg
    11641 caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg tgacggttta
    11701 gtgttgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta cttcagccgc
    11761 gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt tattcgcctg
    11821 ttcaatgtga aaacgccgtc gatggagcag gcaattggtc tgctttccgg tggcaatcag
    11881 caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtgttgat ccttgatgaa
    11941 cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc
    12001 aaagccgatg gcttgagcat cattctggtg tcatcggaga tgccagaagt attaggcatg
    12061 agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac tcgtgagcag
    12121 gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag
    12181 taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat
    12241 ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc
    12301 gaactttttc accatcaata acttattcaa tattctccag caaacctcag tgaacgccat
    12361 tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atcgacttgt cggtaggttc
    12421 tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag tcaatgcgct
    12481 ggtggctgtc gctgccgctc tcgcgttagg tgccgcaatt ggtgcggtaa ccggagtgat
    12541 tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc ttttactgcg
    12601 cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta ctgagaacgc
    12661 cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc cagtctggat
    12721 catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta
    12781 catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca acgtcaataa
    12841 aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcactggccg ggatcattga
    12901 agtggcgcgt ctctcctccg cacaacccac ggcggggact ggctatgagc tggatgctat
    12961 tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac
    13021 gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc
    13081 ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa
    13141 caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac
    13201 cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac
    13261 catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc
    13321 gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc agaacaaccc
    13381 ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat
    13441 taacccgacc gactccgacg cagtaggtaa tgctgtgaag atggctaacc aggcgaacat
    13501 cccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc
    13561 tgataacgta ctgggtggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg
    13621 tgccaaagtt atcgagctgc aaggcattgc tggtacatcc gcagcccgtg aacgtggcga
    13681 aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc agccagcaga
    13741 ttttgatcgc accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
    13801 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcgcg cactgcaaac
    13861 tgccggtaaa tcggatgtga tggtcgtcgg atttgacggt acaccggatg gcgaaaaagc
    13921 ggtgaatgat ggcaaactag cagcgactat cgctcagcta cccgatcaga ttggcgcgaa
    13981 aggcgttgaa accgcagata aagtgctgaa aggcgagaaa gttcaggcta agtatccggt
    14041 tgatctgaaa ctggttgtta agcagtagtt ttaatcaggt tgtatgacct gatggtgaca
    14101 taaatacgtc atcgacagat gaacgtgtaa tataaagaaa agcagggcac gcgccaccct
    14161 aacacggtgg cgcattttat ggacatcccg aatatgcaaa acgcaggcag cctcgttgtt
    14221 cttggcagca ttaatgctga ccacattctt aatcttcaat cttttcctac tccaggcgaa
    14281 accgtaaccg gtaaccacta tcaggttgca tttggcggca aaggcgcgaa tcaggctgtg
    14341 gctgctgggc gtagcggtgc gaatatcgcg tttattgcct gtactggcga tgacagcatt
    14401 ggcgagagcg ttcgccagca gctcgccact gataacatcg atattactcc ggtcagcgtg
    14461 atcaaaggcg aatcaacagg tgtggcgctg atttttgtta atggcgaagg tgagaatgtc
    14521 atcggtattc atgccggcgc taatgctgcc ctttccccgg cgctggtgga agcgcaacgt
    14581 gagcgtattg ccaacgcgtc agcattatta atgcagctgg aatcaccact cgaaagtgtg
    14641 atggcagcgg cgaaaatcgc ccatcaaaat aagactatcg ttgcgcttaa cccggctccg
    14701 gctcgcgaac ttcctgacga actgctggcg ctggtggaca ttattacgcc aaacgaaacg
    14761 gaagcagaaa agctcaccgg tattcgtgtt gaaaatgatg aagatgcagc gaaggcggcg
    14821 caggtactgc atgaaaaagg tatccgtact gtactgatta ctttaggaag tcgtggtgta
    14881 tgggctagcg tgaatggtga aggtcagcgc gttcctggat tccgagtgca ggctgtcgat
    14941 accattgctg ccggagatac ctttaacggc gcgttaatca cggcgttgct ggaagaaaaa
    15001 ccattgccag aggcgattcg gtttgcccat gctgccgctg cgattgccgt aacacgtaaa
    15061 ggcgcacaac cttccgtacc gtggcgtgaa gagatcgacg catttttaga caggcagagg
    15121 tgacgcttgg ctacaatgaa agatgttgcc cgcctggcgg gcgtttctac ctcaacagtt
    15181 tctcacgtta tcaataaaga tcgcttcgtc agtgaagcga ttaccgccaa agttgaagcg
    15241 gcgattaaag aactcaatta cgcgccatca gctctggcgc gtagcctcaa actcaatcaa
    15301 acacatacca ttggcatgtt gatcactgcc agtaccaatc ctttctattc agaactggtg
    15361 cgtggcgttg aacgcagctg cttcgaacgc ggttatagtc tcgtcctttg caataccgaa
    15421 ggcgatgaac agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc
    15481 ttgctgttac tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg
    15541 acagtgccta ctgtgatgat ggactgggct ccgttcgatg gcgacagcga tcttattcag
    15601 gataactcgt tgctgggcgg agacttagca acgcaatatc tgatcgataa aggtcatacc
    15661 cgtatcgcct gtattaccgg cccgctggat aaaactccgg cgcgcctgcg gttggaaggt
    15721 tatcgggcgg cgatgaaacg tgcgggtctc aacattcctg atggctatga agtcactggt
    15781 gattttgaat ttaacggcgg gtttgacgct atgcgccaac tgctatcaca tccgctgcgt
    15841 cctcaggccg tctttaccgg aaatgacgct atggctgttg gcgtttacca ggcgttatat
    15901 caggcagagt tacaggttcc gcaggatatc gcggtgattg gctatgacga tatcgaactg
    15961 gcaagcttta tgacgccacc attaaccacg atccatcaac caaaagatga actgggggag
    16021 ctggcgattg atgtactcat ccatcggata acccagccga cccttcagca acaacgatta
    16081 caacttactc cgattctgat ggaacgtggt tcggcttaga tttacgctgt cttttgatca
    16141 aattattacc atcggttgtt ttcagaagca tgaacattgc tgctgaagca acagtaataa
    16201 tgcccattgt gataaacgta tagtggaatt gttcgacagt cgttgtgcct tccattcctt
    16261 cataaacgcg aaggacggcc gcacttacag ctacgcctaa actgatagac agttgctgcg
    16321 tgaccgccag aacactgtta ccgctgctgg cgttgtcatc ggtcagatcg gcaagtgtga
    16381 tggtattcat cgcggtaaac tgcgtcgaca tagccatccc taatataaac aacggcaaga
    16441 tcagcatcca tattgccatt gccggtgatt gcaaagagaa ctgagcgatc attagcccaa
    16501 taatcaccgt gatccccact aatgtatggc gatagcccag acgacgtaag acttgggtaa
    16561 ccatcgattt tgcaataatg gaacctaacg ctgtcggtgc catcatacag ccagcaataa
    16621 acgcctgata accaaatcct acctgtaaca ttaatggcat aaggaacggt acacagccgg
    16681 tccccagacg ggttgcaata ttgcctacga taccgatcga gaaagtgcgg gttttaaata
    16741 aatctaatga aattaatggg tttggcgtgc gtcgtgcatg gagaatatag agaagcagta
    16801 acccgatgct ggtgacaatt accgtcaagg caatccagct ggcgacaatc ttttccccga
    16861 atagctctat tccgcttgag aagagaacaa ggctgaggcc aaacagcaaa aagccagtga
    16921 tatcgaatct gcgtcgtgcg gtggtgaaat tgggcatatg tttgcgcgcg taaagaaggc
    16981 ccgcaatacc tatggggata ttgattaaaa atatccagtg ccaggttgcc caggtgacca
    17041 gcacgccgcc aagaatgggg cctaaaattg gccccaccag acccggcatg gcgacaaaat
    17101 tcaatactgg aagaagttca ttacgaggat aagcgcgcag taaggccagc cgagcaacag
    17161 gcatcatcat tgcgccgcct atcccctgaa taacccggaa gacaaccagc tgtggtagcg
    17221 aattagaaag tgcgcaggcc agagaaccca atgtgaacag actcacggca agggtaaaaa
    17281 tgcgacgcgt accgaagcga tcggctagcc atccgcttac cggaataagc atcgccaccg
    17341 tcagcgtata actgatgatg gctgattgca tcgcgagagg agaacgatta aggctatgag
    17401 cgattgcggg taaggcggta ttcagaatag tggcatcaag tgcctgcatg aagaaggcca
    17461 tcgccgcgat ccacggcaaa cccgccatac tgcgcttctt tttatcgctc attcaatgtc
    17521 ctgttatcgg gttatcactt atcaggtgag cgtagcagcg cctgacaagc tttaaatgcc
    17581 gcgtcgccat cgctttggat aatcgcatcg actatcgcct gatgcagatc cagctttatc
    17641 actgtgtcgc tggtaattga cgtgaagtaa gtgtgataaa ccgaatggaa tagcgaggcg
    17701 aatgaggtca aaaacggatt ggcgctcatt tcatagatat gctcatgcca ggccatatcg
    17761 acttcgatcc agcgttcacg gcgaaagttc tcttttaatg ccgccatttc ggccattaac
    17821 gtattgagat gcgctttctg ttccgcggtg ccaaccgttg ctgccagtag gcaagcttgt
    17881 ggctccagac agatacgcat aaccagaaag tgatcgatga cctgatgaaa gttctcttct
    17941 gtcatccacc aggtaagcaa ttcctgatca agaaaattcc agttcgattg tggcatgacc
    18001 cgggtaccaa ttcgcggtcg cggtaaaacc atcccttttg ccgttaacgt tttgaccgct
    18061 tcgcgtaccg ctgtacgact cactccaaat tgctcgccca gctcaatctc accgggcaaa
    18121 atggtgccgg gttcatattc accttttaag atccgttgcg ccagcttctc agccagaaca
    18181 tacgaaaggt ttttctgtgc agctaactgt tgtgcgctta aaggcattac taatcttcct
    18241 ttttcttttt attcctcctt agtatgccac caggaagtgt gattacggtt gcaaaaacgg
    18301 caaattgctt gttttatggc acattaacgg ggcttttgct gaaaaaatgc gcggtcagaa
    18361 aattatttta aatttcctct tgtcagaccg gaataactcc ctataatgcg ccaccactga
    18421 cacggaacaa cggcaagcac gccgccgggt cagcggggtt ctcctgagaa ttccggcaga
    18481 gaaagcaaaa ataaatgctt gactctgtag cgggaaagcg tattatgcac accccgcgcc
    18541 gctgagaaaa agcgaagcgg
//
LOCUS       DAZKFZ010000076        18345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000076 DAZKFZ010000000
VERSION     DAZKFZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..577
                     /locus_tag="ACHOQW_002430"
                     /pseudo
     CDS             1..577
                     /locus_tag="ACHOQW_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            680..1183
                     /locus_tag="ACHOQW_002431"
     CDS             680..1183
                     /locus_tag="ACHOQW_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP9573499.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPVKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1250..2707)
                     /gene="nuoN"
                     /locus_tag="ACHOQW_002432"
     CDS             complement(1250..2707)
                     /gene="nuoN"
                     /locus_tag="ACHOQW_002432"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP9573500.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2714..4243)
                     /gene="nuoM"
                     /locus_tag="ACHOQW_002433"
     CDS             complement(2714..4243)
                     /gene="nuoM"
                     /locus_tag="ACHOQW_002433"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP9573501.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4474..6315)
                     /gene="nuoL"
                     /locus_tag="ACHOQW_002434"
     CDS             complement(4474..6315)
                     /gene="nuoL"
                     /locus_tag="ACHOQW_002434"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP9573502.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6312..6614)
                     /gene="nuoK"
                     /locus_tag="ACHOQW_002435"
     CDS             complement(6312..6614)
                     /gene="nuoK"
                     /locus_tag="ACHOQW_002435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP9573503.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6611..7165)
                     /gene="nuoJ"
                     /locus_tag="ACHOQW_002436"
     CDS             complement(6611..7165)
                     /gene="nuoJ"
                     /locus_tag="ACHOQW_002436"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP9573504.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7177..7719)
                     /gene="nuoI"
                     /locus_tag="ACHOQW_002437"
     CDS             complement(7177..7719)
                     /gene="nuoI"
                     /locus_tag="ACHOQW_002437"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP9573505.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7734..8711)
                     /gene="nuoH"
                     /locus_tag="ACHOQW_002438"
     CDS             complement(7734..8711)
                     /gene="nuoH"
                     /locus_tag="ACHOQW_002438"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP9573506.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8708..11434)
                     /gene="nuoG"
                     /locus_tag="ACHOQW_002439"
     CDS             complement(8708..11434)
                     /gene="nuoG"
                     /locus_tag="ACHOQW_002439"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP9573507.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11487..12824)
                     /gene="nuoF"
                     /locus_tag="ACHOQW_002440"
     CDS             complement(11487..12824)
                     /gene="nuoF"
                     /locus_tag="ACHOQW_002440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP9573508.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12821..13321)
                     /gene="nuoE"
                     /locus_tag="ACHOQW_002441"
     CDS             complement(12821..13321)
                     /gene="nuoE"
                     /locus_tag="ACHOQW_002441"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP9573509.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13324..15126)
                     /gene="nuoC"
                     /locus_tag="ACHOQW_002442"
     CDS             complement(13324..15126)
                     /gene="nuoC"
                     /locus_tag="ACHOQW_002442"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP9573510.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15220..15882)
                     /gene="nuoB"
                     /locus_tag="ACHOQW_002443"
     CDS             complement(15220..15882)
                     /gene="nuoB"
                     /locus_tag="ACHOQW_002443"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP9573511.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15898..16341)
                     /gene="nuoA"
                     /locus_tag="ACHOQW_002444"
     CDS             complement(15898..16341)
                     /gene="nuoA"
                     /locus_tag="ACHOQW_002444"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP9573512.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16972..17910)
                     /gene="lrhA"
                     /locus_tag="ACHOQW_002445"
     CDS             complement(16972..17910)
                     /gene="lrhA"
                     /locus_tag="ACHOQW_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP9573513.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4572 a         5112 c         4730 g         3931 t
ORIGIN      
        1 accctgggtc actgcattat cgccaacgcc ccgacacgta attcctgagc aattagaaaa
       61 aacagttcgt tatcttacac aaactgtgca tccacgtaat gccgacaata ttgataatct
      121 gaataggtcc gtagagtata taaaaaaagg tctttgtcag tagcggtgcc agagttaccg
      181 cgcagggcgt ccccaattgg ctattacgac tctgcgcctg gtagccagga ttacccttac
      241 ccgcaatgtc ctggctttat cccgatcggg gggactttat tgcctggtcg gcagaataca
      301 ggatatcaac gccgttcgtc aggtaaaaac ggcattgtta tcatcccggg atttatctgt
      361 ttattctatg aatgccccat ggtttattca cggtattgat ttctctgacc accttaatta
      421 ttggcaacac gatattctcg ccgtaatgat tactgacacc gctttttatc gtaataaaca
      481 ataccacttg cccggtgata cagcagacag attgaattat cagaaaatgg ctcagatggt
      541 ggatggtgtt ataactttgt tacacaacag taaataataa aaatttctcg aggcgggtcc
      601 agaaacagcg acatgtacat tttattttcc ctcgtctgtt ttaagatgcg aaacataaaa
      661 ttcaatggag gaaaaataaa tgggaatgat tggttatttt gcagaaattg actcagaaaa
      721 gattaaccaa ctgcttgagt ctactgaaaa acctttgatg gataatatac atgacacgct
      781 ttcaggatta cgccgactgg atattgataa acgctgggat tttttacact ttgggctgac
      841 aggcacctct gcgttcgatc cagtgaagaa tgatccgctg agtcgtgccg tgcttggtga
      901 acacagcctc gaagatggta tagatggttt cctgggattg acatggaatc aggagttagc
      961 cgcgacaatc gaccgactgg aatcactgga tcgaagtgag ctgcgtaaaa agttttccat
     1021 taagcgactc aatgaaatgg agatttatcc gggagtgacc ttttcagaag agttagaggg
     1081 gcaactcttt gcttcgatca tgcttgatat ggaaaagctg attagcgcgt atcgacgtat
     1141 gctgcgacaa gggaatcatg cgctgacggt tattgttggg tgatggtgtc agggcaccaa
     1201 aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa actgatagct tacatcagcg
     1261 gcattgctaa ccgcacaata ctaatcagcg gttgtggcca tacacccagc accagtacca
     1321 acagtgcaga aatcagcacc acgataccgc ccgcgctgta ctgccagttt gacggtgcat
     1381 cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac cgccacgcgc aggtagtagt
     1441 agaggccgat tgccgaaccg acaaccacgg cacccaccag ccaccacagg tgtgcctgga
     1501 caccaaccgc cagcacgtag aacttaccga taaagcccag cgtcatcggg ataccggcca
     1561 gagacagcat catcaccgtc atcaccgccg cgaggatcgg acgatgccag aacagaccgc
     1621 ggtaagagaa cagggaatca gcatccgggc cacgatacgg gctggacatc aggctgacca
     1681 cgccgaacgc gccgaggctg ctgaacagat aaccggccag gtaaacccct accgcttcca
     1741 tcgacatctc gccagtttgc agcgcaatca gcgccaccag cagatagccg aggtgagaga
     1801 tagatgagta accgagcaga cgtttgatat tggtctggct cagcgccatc aggttaccga
     1861 agatgatgga ggcaaaggcg ataatcgcca gcaccacccg aatcgcttcg ctgtcaccca
     1921 ccggtgcgta gaggaacaga cgcatcacca caccgaagat agcgattttg ctcgccgtcg
     1981 ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga
     2041 acggcaccag agagagtttg aagccgaggc caacaatcat caggccgaaa cctgccagca
     2101 acagcggctc gtttagcata ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt
     2161 cgccagactg cgcatacacc agcgccatac cgaacagcag gaaagaagac gctgcggcag
     2221 aaaggatggt gtatttgata ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga
     2281 ccaggccaaa cagcggcaaa gagatcagtt caataccgag gaacagagac gccagatggt
     2341 ttgcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg tagaactcat
     2401 ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg
     2461 ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc agcggcgtaa
     2521 cgtccatagc gcccgcctgg ccaacaaacc agagcgaaac cagcgccgcg ttaagcccaa
     2581 taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa
     2641 ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt tgtggagtta
     2701 ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac tgctggatat
     2761 tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg atagaagccc agcagtacca
     2821 gcagcaccac cagcaacagg atcataaaca gctcacgcag cgacatccct ggcagttcct
     2881 ggctggcaat ctggcttttc gctttaccga agtaagcgcg atgtaacatc gccagcgaat
     2941 aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg acaacctgga
     3001 agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgcctggc atcccgagcg
     3061 ttgccaccgc aaagaacagt gacaaggctg gcagccattt catcttgctc cacagaccgc
     3121 ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccacaa agaataaaca
     3181 gacccgccgc tgacaagccg tgcgcaatca tctggattac cgcgccctgg taggccaact
     3241 ggctgccggt gtagatagca atcagtacga agcccatgtg ggaaaccgag gtgtaggcga
     3301 tcagacgttt gatatcggtc tgggcgaagg ccatccacgc accgtagaag atgccgataa
     3361 cacccagcca catggcgatt ggcgcgaact ctgccgacgc gtttgggaac agcggtaggg
     3421 agaaacgcag cagaccgtaa gcggcggttt tcagcaagat ccccgcgagg tccacggaac
     3481 cggcggtcgg tgcctgtgag tgcgcatccg gcagccagcc atgcagcgga accaccggca
     3541 ttttcaccgc aaacgcgatg aagaagccca gcatcaacag atattccaca ccactggaca
     3601 ttggcgtatt cagcagctct tcatagttga aggtccagac gccggtcgca ttgtagtgaa
     3661 caaaaaccag cgccaggatg gcgatcaaca tcaccagacc actcgcctgg gtgtaaatga
     3721 agaacttggt tgccgccgtg atacgcgttt taccatcaga ggctttatgc ccccacagtg
     3781 cgatcaggaa gtacatcggc accagcatca tttcccagaa gaagaagaac aggaacatgt
     3841 cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg aggtggaaga
     3901 agccctgata tttttcgatc tctttccacg aacagagtac cgccagcaca ccgagcagac
     3961 cggtcagcac gaccatcagc agcgacagcc cgtcaatggc gagatggata gagataccaa
     4021 aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg gcggattgcg
     4081 tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc aatcccatgg
     4141 tgatcagcgc gatccagcgc ggcaccttga cgccaaagcg ttcggtctgc cagcacagga
     4201 agccgccgat aaaggggatt agtattaacc agggtaataa catagctaag tatgtccctt
     4261 atatttactc ctgctcaggc tatctcactc gccagcccga atcccggcag tgctcgcaat
     4321 cctcacgtac ttaagtacgc tccggttgct gtgcgctggc ggtatccggt ctgacttcgc
     4381 cgatgacgcc tgtacttcag gaacagttac ctaaaattta ttcttagttc acagacccgg
     4441 cgcaacgccg ggtatattca ctcagccaac aactcaacgc agtaccatca acagtgccag
     4501 caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagataac cgttctcgct
     4561 taacagcagg cctttacctg caaagcggga aaggacagcc gggatgttca tcattgagtt
     4621 cagcggatcg cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc
     4681 gtacagccag tcaaagcccc aggcgttgta ccaccaggtg cccagcagac ggcccggcgc
     4741 actgttggcg atggaggtca ccagagtacg tttacccagc cacagccagg ctgccagcag
     4801 aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc
     4861 cagttccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa
     4921 ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg
     4981 agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga
     5041 ggtcataaac gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc
     5101 acccgcgagg atctcatcct tactgaagaa gcccgcagtg actagcggca gtgccgacag
     5161 tgctgcgccg cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc
     5221 catcttgaag atgttctgtt cgtgatggca ggcgagaatg acggaaccgg atgccaggaa
     5281 cagcagcgct ttaaagaacg cgtgggtcat caagtgaaaa atcgccgcat cccatgcctg
     5341 cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg
     5401 tttgatgtcg gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccaac
     5461 aatgcccacc agatgcagaa cttccggcgt catcaggaac aggccgtggg tacgggcgat
     5521 caggtagaca cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg
     5581 gcccgccatc gcgtcggcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc
     5641 accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc
     5701 tggtgccagt tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa
     5761 cagcgcgaaa gcgaggaaca cgtcacccac acgggtcacg acgaacgctt tcattgccgc
     5821 tgcgccattc ttcggatcgg tgtaatagaa cccgatcagc agataggagc acaggcccac
     5881 gccttcccag ccgaggtaca tcagcagcag gttgtcggca agcaccagaa ccaccatgct
     5941 ggcgataaac aggttggtgt aagcgaagaa gcgagagtag ccctcttcac cgcgcatata
     6001 ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag
     6061 cgacaggccg tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt
     6121 ccacagcggc tggctgtatg cctgctcgcc gttagcgaag aagtcaacgc caataaaggc
     6181 agttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaga cgttttcaga
     6241 ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa
     6301 ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga
     6361 cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg
     6421 gcgagaatgt acatcacctg accgtcggtc tggccccagt aacttccggc gaccacaaag
     6481 gccagcgcgg aagcgttaat catgatttcc agaccaatca gcataaacag cagattgcga
     6541 cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt
     6601 tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc
     6661 tcagcacttc acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga
     6721 gcagcagcat agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca
     6781 ctgctttagc actgattggc gtaccgtcga taccttgatc gttaacaccg agaatggcgt
     6841 aaacaatcac caccaacatg atggctgaca aaattgccgg accaatccac acctgcggtt
     6901 tcagccactg gcgttcttgt tcgatttctg aaccgcccag gttgagcatc atcaccacga
     6961 acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt
     7021 aagcgcctag tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca
     7081 gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc
     7141 cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc
     7201 gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc
     7261 catccggtag aagttatact ccgggtattt gcccggaccg gagatcagca gatcctcttt
     7321 ctcgtaaacc agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat
     7381 cgccgtggtc ggacaggctt cttcgcacaa accacagaaa atgcagcgtg agaagttgat
     7441 gcggaaaaat tccggatacc agcgaccgtc tttggtttct gctttttgca gcgagataca
     7501 gccgaccggg caggctaccg cgcagaggtt acaggctacg caacgctctt cgccgtccgg
     7561 gtcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg
     7621 gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac
     7681 ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc
     7741 gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag
     7801 attttccagc cgaaggacat tacctggtca taacgcggac gcggtaacga cgcacgaatc
     7861 aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac
     7921 aacgggcctt gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc
     7981 ccgatgtact cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa
     8041 ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc
     8101 acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggatgacgtt ccacacatgc
     8161 gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc
     8221 agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccg
     8281 agcagcgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga
     8341 ccggccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga
     8401 ctgactggca caatcgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg
     8461 gtaaagatga cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc
     8521 atgtccgcaa ccagctggag cgaaccaccc cagccaacac ggttaggtcc gtaacggttc
     8581 tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccgca ggtgacaacc
     8641 accagcagga tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat
     8701 atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag
     8761 caatgccgga catacccatc ggcaagccca cctgccctgc cgtcagccct tcagcgattt
     8821 caaccggcag agtgaccgtg ttgccatcgt aactaaagga gacgcgtgta cctgcgttta
     8881 cacccaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacgacact
     8941 ggaagaccgg agcacgctgt gacaattcat cgctgccaaa caggtggtag tacggcgcga
     9001 tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac
     9061 cattttcgct ggtttcaaac agacgcacac ccggatcgcc aaagcgcagt ttgccgccca
     9121 cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcccggagcc caggcaaacg
     9181 gcacttgcga acggtgcgcg gtcggctggt tgttaccttc catcgagaag gtgaacatgg
     9241 tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac
     9301 gaccgctgta acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat
     9361 ctggcgcagc atctttgata cccgccagtt ccgggatttt cgccacaaca gcgtcaatta
     9421 catggtcgag ctgcgtccag tccacttcac ggctcagcaa ggtgctgtgc agcgagtgca
     9481 gccagcgcca actttccagc atgacagttt tgctgtcgta ataagccgga tcgtaaacct
     9541 ggaagaaacg ttgggcgcga ccttcattat taattaccgt accgtcgctt tcagcaaagc
     9601 tggcagcgga aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga tggtcaacca
     9661 ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcagaa gcgtgacgat
     9721 gcagatcgtt ttccagcacc accaccgcgt cggcgcgtcc ggtttccagt tcggttaacg
     9781 cttcttcaag cgatccaccg cccataatgc ccaaccccat gctgttgacg gaacgggcaa
     9841 tcatggtgat accgacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag
     9901 cctgaatcac ctctaagcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac
     9961 ccgccagtgc ctgcacgatg acgtcgattt tgctttgcag ctcaggttca ataccgtcaa
    10021 ccgctggcgc agagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt
    10081 caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgggtgtca tcaacgttgg
    10141 taacaaacag cggatgctta gcacgttgac cgatattgag gattgccgca atctgccagt
    10201 cagccacttt ctgtgctgcc gccatttcgc gcgctttacc tttcaccgcc tggcgcactg
    10261 ccagcgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt
    10321 aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg
    10381 ccagttgcag acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca
    10441 ccagttcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca
    10501 ctttcttcga ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg
    10561 taatgaaatc atcgccacga cgctgtactg gctgacgcgg acgatctttc aggttgacgt
    10621 aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac
    10681 ggttttcgat acgacgcagt tcgccataac gctcgccggg gctgatgtta cagccgatgg
    10741 aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg
    10801 agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt
    10861 cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcatgc gcgccataaa
    10921 cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg cacgcagcgg tagcaggcga
    10981 tgcagcggtt catttcgtga gagatgaacg gccccaaatc ctgattacgg tgggtacgtt
    11041 tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc
    11101 agttaccgcc ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga
    11161 ccacactttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg
    11221 aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt
    11281 gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt
    11341 aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct
    11401 cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcagtca
    11461 gagactgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc
    11521 ccattaatca aatgggtatt gctgaacggc tgtttgatcc ccgcctcaaa ttcttcgcgg
    11581 aaatatttga tggcgctctg taacggctcc acagcaccag gtgcgtgggc acagaaagtt
    11641 ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctgacct
    11701 tcgccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc
    11761 gtacaccagc cgcaggactc acgggcgaaa aactcttcca ggttacgcac cagcgacacc
    11821 atgttgattt catggtcaac cgccatcgcc agcgccgtac ccagacggct gcccgcttta
    11881 ccgatacttt cgaattccat cggcaggtca aggtgcgctt cggtcaggaa gtcggtgccc
    11941 gcgccgcctg gctgccaggc tttgaatttc agaccgtcac gcataccacc ggcgtaatct
    12001 tcgaggatct cgcgtgcggt ggtgccgaac ggcagttccc acagtcccgg atttttcacc
    12061 cgaccggaga agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac
    12121 cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca
    12181 caggtcggtt tgccccatac gccggaggtt gccgggaagg ggggtttcga gcgtgggtta
    12241 gcacgacgcc cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct
    12301 gccccggtat ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt tttgccaagc
    12361 aatcccgctt cagtcgcttc ggcaatggcg cggcgcagat ttactgccgc ttcaatatat
    12421 tcgccacgca ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata
    12481 ccttccacca gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc
    12541 tccatttcat cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc
    12601 aggctccact tcaagccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct
    12661 tttacctgat taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg
    12721 taaccgtttt tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc
    12781 caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca
    12841 gttcagggat cgcttccggg gtcagattcg cgtgagtgtc ctcatcgatc atcatgtttg
    12901 gccctttatc acagttcccc aggcagcaag ttggcagcag cgtaaagcgg ccatcaaatg
    12961 tcgtttgccc tggtttgatg ttcagctttt tctcgagcgc cgcctgaata ccctgatacc
    13021 cattgatatg acagaccacg ctgtcgcaat aacggatcac atggcggcca accggctggc
    13081 ggaagatctg gctgtagaac gttgccacgc cttcgacgtc gcttgccgga atacccagca
    13141 catcggcgat cgcgtggatc gcaccatccg gcacccagcc acgctgcttc tgaacgattt
    13201 tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcgatcg
    13261 cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca
    13321 taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc
    13381 agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt
    13441 gcggatacga gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat
    13501 ccctttggtc gcctcaatca tctggaagga ttcattggca ggcatcaccg gcccccacga
    13561 cacttgcagg aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg
    13621 cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg
    13681 ctcaagaatg cgcagactct ggcgcagctc ttccacttta agcattacgc gggtgtagca
    13741 gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg
    13801 acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc
    13861 ccactccagc gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat
    13921 ggtattttgc agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa
    13981 ctcacgcagc aggcgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa
    14041 ccacgccggg tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg
    14101 atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat
    14161 atacagcagg tggctgttga tgcggaacag ttcggagagc attacgcgaa tgacgttaac
    14221 gcgagccggc acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt
    14281 aacgcagccg ccgaggtatt cgatacggtc ggtatacgga atgtagctgt gccaggactg
    14341 gcgttcgccc attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac
    14401 aatctcttcg ccgtcgagct gcaaaacgat acggaaagca ccgtgcgccg acgggtggtt
    14461 cggaccgagg ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc
    14521 ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa
    14581 cggcgagaat tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt
    14641 ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aatgtaatgc caaacagatc
    14701 ccaggtttca cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg
    14761 caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat
    14821 cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgcg tgcgcagacg
    14881 ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa
    14941 atcgccaact tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt
    15001 cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc
    15061 atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt
    15121 caccattgca aatggtccgt ggtgactgtc ggcgcagcaa gcttcgcgaa atagatttag
    15181 gaataagcga agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg
    15241 caggttggtt acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt gcatattggc
    15301 gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga
    15361 ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat
    15421 atacacatca accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat
    15481 accaccagag ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc
    15541 atacagacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag
    15601 gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac
    15661 cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca
    15721 aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt
    15781 gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc
    15841 gttctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta
    15901 gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc
    15961 gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa
    16021 aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca
    16081 tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa
    16141 cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac
    16201 gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca
    16261 acataggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc
    16321 agtggatgtt gacatactca ttgcttactc atcaaaagta gcgccagatt cactgctctt
    16381 cacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaatcact
    16441 gtagaaattc ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg
    16501 tcaactttaa caaaagtttc ttcacattag tttacataat atcaacacca ttagcattta
    16561 atgccctttc accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt
    16621 ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct
    16681 ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgtcattcc ataacaaaaa
    16741 cgcagcaaca aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga
    16801 ttatttgtgc gtacggggcg tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt
    16861 ctgcacgatg tgaagaaggt tctttactaa aaaaaagccg ctggggttta aaacaccccc
    16921 agcggctcgt tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat
    16981 atccctttca atcaacaatg aatcatcccc ttccggagca gatattgggc tgtattgcca
    17041 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
    17101 cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact
    17161 cagcacacgc aagtccggac tcatcatctc gaccggcctt gccgtcacgc caagaccggc
    17221 tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccagggaat
    17281 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa
    17341 tacaagaggg atcggctcac ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
    17401 tggcgaggta cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa
    17461 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
    17521 cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
    17581 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
    17641 tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc
    17701 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
    17761 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
    17821 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
    17881 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
    17941 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
    18001 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
    18061 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata
    18121 tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat
    18181 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
    18241 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
    18301 aaccttttat ttataagggt cattgcgaat tatctgatgc aaagt
//
LOCUS       DAZKFZ010000077        18260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000077 DAZKFZ010000000
VERSION     DAZKFZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(55..813)
                     /gene="aroD"
                     /locus_tag="ACHOQW_002446"
     CDS             complement(55..813)
                     /gene="aroD"
                     /locus_tag="ACHOQW_002446"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9573514.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(844..1710)
                     /gene="ydiB"
                     /locus_tag="ACHOQW_002447"
     CDS             complement(844..1710)
                     /gene="ydiB"
                     /locus_tag="ACHOQW_002447"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP9573515.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1722..2987)
                     /locus_tag="ACHOQW_002448"
     CDS             complement(1722..2987)
                     /locus_tag="ACHOQW_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9573516.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3214..4428)
                     /locus_tag="ACHOQW_002449"
     CDS             complement(3214..4428)
                     /locus_tag="ACHOQW_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9573517.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4528..4884)
                     /locus_tag="ACHOQW_002450"
     CDS             complement(4528..4884)
                     /locus_tag="ACHOQW_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP9573518.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5020..5127)
                     /gene="rprA"
                     /locus_tag="ACHOQW_002451"
     ncRNA           complement(5020..5127)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOQW_002451"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5313..6425)
                     /gene="ydiK"
                     /locus_tag="ACHOQW_002452"
     CDS             complement(5313..6425)
                     /gene="ydiK"
                     /locus_tag="ACHOQW_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP9573519.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIQKPNK"
     gene            6814..9870
                     /locus_tag="ACHOQW_002453"
     CDS             6814..9870
                     /locus_tag="ACHOQW_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP9573520.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9867..10277
                     /gene="menI"
                     /locus_tag="ACHOQW_002454"
     CDS             9867..10277
                     /gene="menI"
                     /locus_tag="ACHOQW_002454"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP9573521.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            10377..10565
                     /locus_tag="ACHOQW_002455"
     CDS             10377..10565
                     /locus_tag="ACHOQW_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP9573522.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11114..11482
                     /gene="sufA"
                     /locus_tag="ACHOQW_002456"
     CDS             11114..11482
                     /gene="sufA"
                     /locus_tag="ACHOQW_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP9573523.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11491..12978
                     /gene="sufB"
                     /locus_tag="ACHOQW_002457"
     CDS             11491..12978
                     /gene="sufB"
                     /locus_tag="ACHOQW_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9573524.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12988..13734
                     /gene="sufC"
                     /locus_tag="ACHOQW_002458"
     CDS             12988..13734
                     /gene="sufC"
                     /locus_tag="ACHOQW_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9573525.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13709..14980
                     /gene="sufD"
                     /locus_tag="ACHOQW_002459"
     CDS             13709..14980
                     /gene="sufD"
                     /locus_tag="ACHOQW_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9573526.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            14977..16197
                     /gene="sufS"
                     /locus_tag="ACHOQW_002460"
     CDS             14977..16197
                     /gene="sufS"
                     /locus_tag="ACHOQW_002460"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP9573527.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16210..16626
                     /gene="sufE"
                     /locus_tag="ACHOQW_002461"
     CDS             16210..16626
                     /gene="sufE"
                     /locus_tag="ACHOQW_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP9573528.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16775..17779
                     /gene="ldtE"
                     /locus_tag="ACHOQW_002462"
     CDS             16775..17779
                     /gene="ldtE"
                     /locus_tag="ACHOQW_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP9573529.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17842..18078)
                     /gene="lpp"
                     /locus_tag="ACHOQW_002463"
     CDS             complement(17842..18078)
                     /gene="lpp"
                     /locus_tag="ACHOQW_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP9573530.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4791 a         4421 c         4688 g         4360 t
ORIGIN      
        1 gaatatattt ttttagttcg gcgggggagg atattcccgc cgaaatatta ttgcttatgc
       61 ctgatgtaaa atagttaata ctgtgcgcaa atcatttacc gagatttgcc ctggcgcaga
      121 ggcttttttt accgcaccaa aagttgccgc cgagccaaat acttcaccag ccagacgaga
      181 aattacgcca gtttttgcca ttgacatcgt gatgattgga cgatcggcat actgctcctg
      241 catctccagg gtcgcggcaa gcaacgccag cacatcgctg gtactttgcg gcatcagcgc
      301 aatcttagga atatcggcgt cgaaggactg cattttgcgc agacgggcaa tgatttcttc
      361 ggcttccggc gttttatgga agtcatggtt ggacatgaca actttcacat catgcgcgtg
      421 ggcgtaggcg acggtttctt taacctgatc atcgccggta aataactcca gatcgatcat
      481 atcaaccagg ccgctgtcga tggctgcacg attgagagca atataagcct cggtggaaat
      541 cgcctgctcg ccgccttctt tggcactgcg gaaggtaaac agcagcggtt tttctggcat
      601 ggtttcacgg agaatttttg ccgccgccat gacagactcc acattggaga ggtcggcaaa
      661 gtggtccaca cgccattcca gaatatcaaa gtccgcttca cgataggcga gagcttcgga
      721 tttcactcgg gcgatatctt tcgccatcag cgagacgatg attttaggtg cgcccgtacc
      781 aatgacgaga tcttttacag ttacggtttt cattttttac cctttctgca cgcggtcagc
      841 ctgtcaggca ccgaacccca tgacctgttt aacatattcc agagggaaat ctttgccagt
      901 ccataacgtg aactgttcag ccccttgcca caacaacatg ccgtatccat caatcgtttt
      961 gcaaccagct tgttgcgcct gctgcaataa cttcgtcata tgcgggttat acacgcattc
     1021 agtgaccagc agtcccggat gtaacagact gatatcatta accaatgatt tattctcaag
     1081 gggtttcata cccacttttg tgccattggt taaaatgtcg gcggaagcca gggcttcagc
     1141 aaaggcttgc tgatcggcga gatcggtaac cgtgacgaca caatcggtgt tttcgttaac
     1201 ccgctgcgcg aaggcgaggg ctttatcgaa gaacttatcc cgacggttaa agagtttaat
     1261 ttcttttaaa ccttcaattg ccccctgcgc gccaatcgcc gttgaggcac ctccggcccc
     1321 taacagcacc attgttttgc ctttgatatc aaaaccgctc tctttaatgg cgcgaatatg
     1381 gcccgtgccg tcggtgttat agccgcgcag gtagccatca tcattaacga tggtgttgat
     1441 ggcacccacc agtttggctg ctggcgttaa ttcatcaaca tattcacacg ccagttgttt
     1501 gttcggcatt gatacaccag ttccgcgcat ttttagagct tttaatcctt caattgctcc
     1561 gggaaagcta tcgttatcca cttcgaaggc catataggta aatggcaatc ccgctttttc
     1621 taaggcttta ttctgcattt caggcgataa actgtggcgg ataggataag ccatcaaccc
     1681 aatcaattcg tattttgcgg taacatccat aattaactcc tttaacctct atgcttaatt
     1741 gtgtttaacc gggtgctaaa tttgcgctcg ccaaaccgca catcattctc aggaataata
     1801 aaaacgcggt aatagcggat aaaaacaata attgcggtaa tcagggccag cagcgcgaaa
     1861 gtaaaatcga gaacaatgat atattgcagg ccgatgttcg acagataacc ggtaatcagc
     1921 ggaataacaa agttagccag tccacccatc atcatataaa tactggtgac tttggctttg
     1981 ctttttggga aaaactctga catgaccgaa acaccgagct gtaaaatgcc gccagctgct
     2041 gagaaaccga taacaaaggc gccggcatta cacaccagtg gagaagggta caggtagata
     2101 atggctgctg ttattgttgc cagtgcagag ttaaatacat tagcccagat gggccggacc
     2161 atttttttca gtagtgcggc aaaaataaag acacagacca gcgagcccat actgtaataa
     2221 gagatggttt ttaatgcctc agcttctgac ataccagcaa aagccatcgc atatttgggc
     2281 atccacacca caatcacata aaaggtcgag aatgcggcta caccgaacag taccgatgaa
     2341 acaccttcca gccagactaa cggtttgctg ttcatttgcg gtaattcatt agctacgctg
     2401 gcgtccacca actggctggg gaatttgctt ttcaacagca tcagcgtgat cagtacaaac
     2461 agaatacccg gaataatcag cccatagccg taccagatat tattgagcaa catatagctc
     2521 accagcattg ggtagaacat ttgcccaaat gacaccatcg ctttaaccag tatgaccgcc
     2581 gaaccagagg ctttcggaaa gcattccatg agcgcggggt agccacccgt atccagcgct
     2641 gagttagcga tacctacgca cactgccaga ccgtaggcga gagttaaatt cgggcaagcg
     2701 ggaataccaa agaagaatag cagatacatt attactgcca ttaatatcac cgcccgacga
     2761 ccaaacttat cggagatcac accgaagaat aaaatactga tcaatcgccc caaaccgata
     2821 ccggaaatta agtaggcaat gcccgcgttg tcagtggaaa acttttccgc cagagatgac
     2881 atattttggg caagcgtaat aacactaatg ccgtgcagga agtagctgaa gtaaatacaa
     2941 agaacagcca ggataaatgg cgtgctgaaa gccttatttt gagacatttt cacagctcct
     3001 tgctgaaaga taatttcgtt accgtacgta ttgttctccg acggtttaca taattactat
     3061 actgaagatg attttccctg ggtacatggc ataaagttta tgtttccagg aatattgcat
     3121 gtcatagttt gtttttgatc gcgattttcc ttagttagcg cagaaagagt tatttatccg
     3181 taagatcttg catgacctga aaagaatatt gcattaccca cccggagcga cattttcctt
     3241 tagcgagtga tgccgcgttt ttttgcggct gcgtaagccg ataaacagtg ccagtaaaaa
     3301 accgatggca gcgatggcgg tatcgaacca cataatatcg gcaatacttc tttgggacag
     3361 atgagccgta atcaacggaa tagtaaaggt cgcaatactg cccgcactgt aatagatccc
     3421 tgtagcttta cctttagcgt aagggaaacg ttcagccatt aacgtcaggc caatttgcac
     3481 aacacctcca gcagaagtaa aaccaatgac aaaagaaaat attatcacca cataaaatgt
     3541 gggatgcagg cagacggtaa acagagcaat aaatgagata aaggtgtaca gcatcagtaa
     3601 tgttgttggg cgaacggtat tacgaatgag tggagcggta ataaatacac aaagcagcga
     3661 acccacggtg tagatactga gtagtttgat cgacatagtg tatgacatgc ctgcaacaaa
     3721 ttgtccgtac tgtgccagcc actggctaac cagataaaac gttgccattg agatatagcc
     3781 atataaggta taactggcta aatcgataat tgaacagcga tgttccgtag agctggtggt
     3841 tttctttatg acaggtaagc gacgacccgg atggggtggg aacgtacaac ggtataaaaa
     3901 cagagcgtta ataaacataa tgcctgcagc aatcataaag gaccaaccga accacagttc
     3961 agcccacact aacaggctaa tgattagcgg taataaaaat tgtccgctgg aaacaaatgc
     4021 tttaattaaa atattggctg tcccaggtga gcgtggaaaa gcttccatca aactgggata
     4081 agtgcctgca tcgagaaaac tgtttgccat tcccgccaga aagccaaaaa cataagcgat
     4141 aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc atatagcagc acatcccgag
     4201 catgataaaa gggcggcgac caaagcgatc ggataataat cctgcaaaaa gcaagacact
     4261 taatcgacca atgcccagcg atgagataac tatcgagaca cccgcggcat tagactgcca
     4321 aagtgtctcc agcgaggcca tattcaggct catcaaaatg acgcccatac catgcaccag
     4381 gtaattaaaa tacaacccaa gtgcggtagg gtaataggga tttttcatag gttctactta
     4441 gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta tccgttcatt attgttgtaa
     4501 caacgacaat tcctttttgt ggagaaatta acaaagccgc tccagatcgt gtgcatataa
     4561 ttcagcggct accgattgat agatcttcca gtcaataaaa ttgccatcag ggtagacttg
     4621 ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg ttgccgatgc ggttatttat
     4681 tttctctatg atcgcatgaa gatgtttttt tctttgctga cgcatagcca acagttgctc
     4741 gactacacaa tcaggaatgg cacacctgcc attttcccat tgacgccagc tttcactgtc
     4801 accggtctgg gcaatccagg tcgcgcattc atcaatagtc atggcaaaaa tatggcggag
     4861 tgcctggagt tcataagcgt tcatcgtgta atggctgcat gaaataaaca ataaaatata
     4921 aataacatta tcagcctgct gacggcttga agagagtcac agtatcttgt gcaacattat
     4981 tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt taaaaaaaag cccatcgtgg
     5041 gagatgggca aagactacac acagcaattc gttgtttcac tcaggggatt tccatgctta
     5101 taaatcaata tgttgattta taaccgtgag ctaatagtag gcatacggac tttttgcgtc
     5161 gatcagattc gtctcattag tttaaaaact gtaaagaatt tgcgacaggt actaccagct
     5221 gaatggcggt aagcaggaaa atatggggaa gagcaagcaa atatcaatta gcggtggtta
     5281 acaatcaggc ggcagagccg cctgatgaga aattacttat tcggtttctg gatttcgcca
     5341 agttcttcga gaatttcttc cggttgatcc gtcggcggtg ggacctcttc cacccacgcg
     5401 gcaaacagac gccaggaaac ggctaacaga accggaccaa taaacagacc gatcatcccg
     5461 aaagcaatca aaccaccaat aacgccagag aggatcagga tcagcggtaa atcggcaccc
     5521 atgcgaatta acattgggcg gatgacgtta tccagcgtgc caaccacacc gctccacact
     5581 aacaataccg ttccccaggt ggtatcgcca gtccagtaga gccagataat cgccggaatc
     5641 agtaccggca acgggccaag ctggacaagg caggaaagga tcattaacac cgttagcaaa
     5701 gttgcataag gtacgccgga tacggcgagg ccgataccgc caagcactgc ctgtactaac
     5761 gccgtcacca ccacacccag cgccaccgcg cggatagcct gtgccgccag cagtacggcg
     5821 gcatctccgc gaacgcctgc cagacgggtc gcaaaatggc gaatgccttg tgccacctgt
     5881 tcaccgcgcc agtacagcag ggcactgaag agaagcatca gcgcacaatg caccataaag
     5941 cgcccgatat gcgccgcttg cccaacgaac caggtagtgg tggtgccaat ataagggcgg
     6001 actttcgcca tgatcgccgt cccccccata tccagcaagt tgtgccagcc tgcatacagc
     6061 ttcgcgccaa tcaccggaat ggtattaagc cacgccagat cgggtaacgt catgtcaccg
     6121 ctggaaatgg ctttaattag cgggccgctg ccgtcgacga tactgttaac cagcaaagca
     6181 atagggatga taaacaccat cactaataac agcgtcatca ccagaacggc gagagagcgg
     6241 cggccaaaca tgatcttttg caaacgtaac aataccggcc aggtggcgat aaccaccgta
     6301 ccggcccatg caaagccgag aataaagggt tgaacaatcc acagacatgc cacaatcatg
     6361 atggctaaaa acagcaccga aagcagaatt tgtgcgacat ccctgggctg acgaacattt
     6421 accataacta cttttcacct tttttgtgcg tcaaatcgtc gacgcgacgt gaacacgcga
     6481 aactcacctg cataatgatg agcaatttca gcggttttta acaggccgat tctgccctta
     6541 attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac tcaggcaaca cgcaaacgat
     6601 ttactcgtcg tatttcaagt tgcatatgct gcggctacgt tcgttcaccc cagttactta
     6661 ctgttgtaag ctcctggagt tcactcgctt gccgccttcc tgcaactcga aatccataga
     6721 gtaaataaac atcacctttc tgtgcgctat tgcagccgcc tgaaagaaca gtgtatcgac
     6781 ctacatcaca gaccgcagga aagggtcaat ataatgattc cacagatttc ccaggcaccc
     6841 ggcgtcgttc aactggtgct taattttttg caagagctgg agcaacaagg ttttaccggc
     6901 gatacggcga caagttatgc tgatcgtctg acaatgtcga ccgacaacag tatttaccaa
     6961 cttctccccg atgcagtggt atttccgcgt tcaaccgcag atgtggcgct gatcgcccgt
     7021 cttgccgcgc aggaacgcta ttcatcgctg atctttaccc cccgcggcgg cggcaccggc
     7081 actaacggtc aggcgctcaa ccagggtatt attgttgata tgtcccgcca tatgaaccgc
     7141 atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg aggccggggt gataaaagac
     7201 caactcaatc agtacctgaa accgttcggc tacttttttg caccagaact ttcgaccagc
     7261 aaccgggcaa cgctcggcgg gatgatcaat accgatgcat ccggtcaggg atcgctggtc
     7321 tatggcaaaa cgtcagatca cgtacttggc ctacgtgcgg tattgttggg gggcgatatt
     7381 ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc tgggtaaatc caataccaca
     7441 atcgggcgaa tttataacac ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc
     7501 gacaacttcc ccaaacttaa ccgctttctt accggttacg atctgcgtca tgtctttaac
     7561 gatgagatga ccgagttcga tctgacgcgc attctgacgg gttcagaagg gacgctggcc
     7621 tttattaccg aagcgcggct ggatattacg cgcttgccta aagtgcgccg tctggtgaat
     7681 gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg
     7741 gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc tggcgcggga agatattgtc
     7801 tggcattccg tcagcgagtt gattaccgat gtgcctgaca aagagatgct cgggctgaac
     7861 attgtggaat ttgctggtga tgatgaggcg ctgattgacg agcgggtaaa tgcactctgt
     7921 gcgcggttgg atgagctgat cgccagtcat caagcaggtg tcatcggctg gcagatgtgc
     7981 cgtgagctgg cgggggttga acgtatctat gcaatgcgca aaaaagccgt tggtctgctt
     8041 ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg aggatacctg cgtaccgccg
     8101 gaacatctgg ccgattatat tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc
     8161 tacggtatgt tcggtcacgt cgacgcaggt gtcttgcatg tccgtccggc actggatatg
     8221 tgcgatccgc aacaagagat tttgatgaag caaatctctg atgacgtggt ggcgctgact
     8281 gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag gttttcgcgc tgaatacagc
     8341 ccggcgtttt tcggtgagga actttttgca gaactgcgca aagtgaaagc ggcatttgac
     8401 ccgcataacc gactcaaccc agggaagatt tgcccgccag aaggtctcga tgcgccgatg
     8461 atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc agatccccat tgcggtacgc
     8521 cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgcc
     8581 cgtagtccga tgtgtccgtc gatgaagatc acccagaacc ggattcattc accgaaaggg
     8641 cgcgcaacgc tggtgcgtga atggctgcgt ttgttggcgg atcgcggcgt tgatccactc
     8701 aaactggaac aagaactgcc tgaatccggc gtcagcttgc ggacgttaat tgcccgcacg
     8761 cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct cacacgaagt caaagaggcg
     8821 atgtcgggct gtctggcctg taaagcttgt tcgacccagt gccccatcaa aattgatgtg
     8881 ccggagtttc gctctcgttt tctgcagctc tatcacaccc gttatttacg cccgctgcgc
     8941 gaccacctcg tcgctacggt cgagagctac gcgccgctga tggcacgcgc gccgaaaacc
     9001 tttaacttct tcattaacca gccgctggtg cgcaaactct cggaaaaaca tatcggcatg
     9061 gttgatttgc cgctgctgtc ggtcccctcg ctacaacaac aaatggtggg gcatcgctcg
     9121 gcaaacatga cgctggaaca gcttgaatct ctcaatgcag agcagaaagc gcgcacagtg
     9181 ttggtggtgc aggacccctt taccagctat tacgatgcgc aagtggtggc ggattttgtc
     9241 cgtctggtcg aaaaattagg tttccagcct gtgttactgc cattttcgcc aaatggcaaa
     9301 gcccagcata ttaaaggttt tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat
     9361 ttcctcaacc gtatggcgaa gctgggtatg ccaatggtgg gcgtcgatcc ggcgctggta
     9421 ctttgttatc gcgatgaata taaactggcg ctgggcgagg agcgtggcga gtttaacgtc
     9481 ttactggcga atgaatggct ggcaagcgca cttgagtcac agccagtggc tacagtcagc
     9541 ggtgaatcat ggtatttctt tggtcactgt accgaagtta ccgccttgcc gggtgcgcca
     9601 gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggt
     9661 tgctgcggca tggcagggac ttacggacat gaagcgaaaa accataaaaa ttcgctcggg
     9721 atctatgagt tatcctggca tcaggctatg cagcgactgc cgcgtaaccg ctgtctggcg
     9781 accggatatt cctgccgtag tcaggtcaaa cgggttgaag gcacgggggt acgccatcct
     9841 gtgcaggctt tactggagat tattaaatga tatggaaacg aaaaatcccc ctggaagcac
     9901 tgaatgccat gggtgaagga aacatggtgg ggttactgga tattcgcttt gaacatattg
     9961 gtgatgacac ccttgaagcg acaatgccag tagactcgcg gacaaagcag cctttcgggt
    10021 tgctacatgg aggtgcatcc gtggtactgg ccgaaagtat cggttccgtt gccggttatt
    10081 tatgtaccga aggtgagcaa aaagtggttg gtctggaaat caatgctaac cacgtccgct
    10141 cggcacgaga agggcgggtg cgcggcgtat gcaaaccgtt gcatctcggt tcgcgtcacc
    10201 aggtctggca gattgaaatc tccgatgaga aagggcgttt gtgctgttcg tcacgattga
    10261 cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac gcatattgct tttggtcatt
    10321 atctgcacag taaacggtgg tatactgtgc aggtcttgtc taaaagcgag gatgctatgt
    10381 ctactcaact tgatcccacc cagctggcca ttgaattttt acgccgtgac cagagcaatc
    10441 tttcacctgc ccaatatctt aaacgtctga aacaactaga acttgaattt gctgacctgc
    10501 ttactctctc ttctgctgaa ttaaaagaag aaatctactt tgcctggcgt ctgggcgtgc
    10561 attgatcctg acttaatgcc gcaagttcgc caaatcgacc tggattaacc tccgtccagg
    10621 ttcgatgacc ttcttaatac tgcttatcta cccgcctgat attgttcaaa aataatacta
    10681 atcgcagttt gtgttaaaac ggcggcttag ctttatgaga ttattcttat cgccccttca
    10741 agagctaagc cactgagagt gccggagata agcgccggat ggggtagaaa cccttaagcc
    10801 tgtgtcgcac agacttaagg gttttcttat ttctgaacgc tgcacttttt tacctatctc
    10861 ttaaatagtc attttcaata caactttttc tttttcatca cagccttaat gctccttaga
    10921 taataatcat tatctaacaa tgagatacct aattcttagc gtgcctgtta acccacacat
    10981 cagggtctat gcttattaaa agcaggcaaa aagcatgttt tacggtaaag cccctgcctt
    11041 tgctgggttg aactgataat cattatcact aacatgctgt tatacgctga aagcgatgaa
    11101 gtgaggtaaa tcgatggaca tgcattcagg aacctttaat ccacaggatt tcgcctggca
    11161 aggcttaacg ctgacacccg cagccgctgt ccacatccgc gagctggtgg caaagcagcc
    11221 gggtatggtc ggcgtgcgct taggtgtgaa gcaaacgggc tgcgcgggct ttggctatgt
    11281 gctcgacagt gttagcgagc cggacaaaga cgatctgctg tttgaacacg acggcgcgaa
    11341 gctgtttgtc ccgctgcaag cgatgccgtt tattgatggc acggaagtcg atttcgttcg
    11401 tgaaggactt aatcagatat tcaaatttca caaccctaaa gcccagaatg aatgtggctg
    11461 tggcgaaagc tttggggtat aggcggtact atgtctcgta atactgaagc aactgacgat
    11521 gtcaaaacct ggaccggcgg cccgctgaat tataaagaag gattcttcac ccagttagcc
    11581 accgatgagc tggcaaaggg gataaacgaa gaggtggtgc gcgcaatttc ggcgaagcgt
    11641 aatgagccgg agtggatgct ggagtttcgt ctaaacgcct atcgcgcatg gctggagatg
    11701 gaagaaccgc actggttgaa agcgcactac gacaagctga attatcagga ttacagctac
    11761 tactcggcac catcgtgcgg taattgtgac gacacttgcg cgtctgaacc tggcgcggtg
    11821 cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg aggcggcgtt tgagcagttg
    11881 ggtgttcccg tgcgggaagg caaagaggtg gcggtggatg ccattttcga ctcagtttcg
    11941 gttgccacta cttatcgcga aaaactggcg gagcagggaa ttattttctg ttcctttggt
    12001 gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc tcggcaccgt ggtgccgggg
    12061 aatgacaact tctttgccgc gcttaatgcg gcggtagcct ctgacggtac gtttatttat
    12121 gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct attttcgcat taacgcggaa
    12181 aaaaccggac agtttgagcg caccattctg gtggccgacg aagacagcta cgtcagctac
    12241 attgaaggct gttccgctcc ggtgcgtgac agctatcagt tgcacgcggc agtggtggaa
    12301 gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg tacaaaactg gttccctggc
    12361 gataacaaca ccggcggtat tctcaacttc gtcaccaagc gtgctttgtg cgaaggcgaa
    12421 aacagcaaaa tgtcatggac gcaatcagaa accgggtcag cgattacgtg gaaatatccc
    12481 agctgcattt tgcgcggcga taactccatt ggtgagtttt actcagtggc gctgaccagc
    12541 ggtcatcagc aagcggatac cggcaccaag atgatccaca tcggtaaaaa caccaaatcg
    12601 accattatct cgaaagggat ctctgccgga catagccaga acagttatcg cggcttagtg
    12661 aaaatcatgc cgacggcaac caatgcgcgc aatttcactc agtgcgactc aatgctgatt
    12721 ggcgctaatt gtggggcgca taccttcccg tatgtcgaat gtcgcaataa cagcgcacaa
    12781 ctggagcacg aggcaacgac atcacgtatt ggtgaagatc aactgtttta ctgcctgcaa
    12841 cgcgggatca gcgaagaaga cgccatctcg atgattgtta acggtttctg caaagacgtg
    12901 ttctcagaac tgccgctgga atttgccgtt gaagcacaaa aactcctcgc catcagtctt
    12961 gaacacagcg tcggataagg aataaacatg ttaagtatta aagatttaca cgtcagcgtg
    13021 gaagataaag ctatcctgcg tggattaagc ctcgacgttc gtcccggcga agttcacgcc
    13081 attatggggc caaacggttc gggcaaaagt accttatcgg caacgcttgc cgggcgagaa
    13141 gattatgaag tgacgggcgg cacggttgag ttcaaaggca aagatttgct tgcactgtcg
    13201 ccggaagatc gtgcaggcga aggcatcttt atggccttcc agtatccggt ggagattcca
    13261 ggtgtcagta accagttttt cctgcaaacg gcacttaatg cggtgcgcag ctatcgcggc
    13321 caggaaacgc tcgaccgctt tgattttcag gatttgatgg aagagaaaat cgctctcctg
    13381 aagatgccgg aagatttatt aacccgttcg gtaaacgttg gtttttccgg cggcgagaaa
    13441 aagcgcaacg atattttgca aatggcggtg ctggaaccgg agttatgcat tcttgatgaa
    13501 tcggactccg ggctggatat cgacgcatta aaagtggtcg ccgatggcgt gaactcgctg
    13561 cgtgatggca agcgctcatt catcattgtt acgcactacc aacgcattct cgactacatc
    13621 aagcctgatt acgttcatgt gctatatcag ggacgaattg tgaaatccgg cgatttcacg
    13681 ttggtcaaac aactggagga gcagggttat ggctggctta ccgaacagca gtaacgcgct
    13741 gcaacagtgg catcacttgt ttgaagctga agggacaaaa cgctccccgc aagcacagca
    13801 gcatttacaa caattgctgc gtaccggact gccgacacgt aaacatgaaa actggaaata
    13861 tacgccgctg gaagggctga ccaatagtca gtttgtcagc attgcgggag agatatcccc
    13921 acagcagcga gatgccttag cgttaacgtt agacgctgtg cggctggtat ttgtcgatgg
    13981 acgttacgtg cctgcgctga gcgatgcgac tgaaggcagc gggtatgaag tgagcattaa
    14041 cgacgaccgt caggggttac ccgatgctat tcaggcggaa gtgtttctgc atttgacgga
    14101 aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc ggtcaacggc cggcaaagcc
    14161 attgctgtta atgcatatca cccagggcgt ggcaggtgaa gaggtgaaca ctgcccatta
    14221 ccgacatcat ctggagctgg cggaaggtgc cgaagcaacg gtgatcgaac attttgtcag
    14281 ccttaatgat gctcgtcatt ttaccggggc acggttctct atcaacgtcg cagcgaatgc
    14341 ccacttgcag catatcaagc tggcgtttga aaacccgctc agtcaccact ttgcccataa
    14401 cgatttgttg ctggctgacg atgccaccgc atttagccac agtttcctgc tgggtggcgc
    14461 agtgttacga cacaacacca gtacgcaact caatggcgaa aactgcacgc tgcggatcaa
    14521 tagcctggcg atgccggtga aaaacgaggt gtgtgatacc cgtacctggc tggaacacaa
    14581 taaaggtttt tgtaacagcc gacagttgca caaaactatc gtcagcgaca aaggccgcgc
    14641 ggtatttaac ggtttgatca acgtcgcgca gcacgccatc aaaacggatg gtcagatgac
    14701 caacaataat ctgctgatgg gcaaactggc ggaagtggat acgaaaccgc agctggaaat
    14761 ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg gggcgtattg atgatgaaca
    14821 gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat gcccagcaga tgatcattta
    14881 cgcctttgct gccgaactga cggaagcact gcgtgatgag gggcttaaac agcaggtgct
    14941 gacccgaatc ggtcaacggc tgccaggagg tgcaagatga ctttttccgt cgacaaagtg
    15001 cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt tgccgctggc ttatctcgac
    15061 agcgccgcca gtgcgcagaa accgagccag gtgattgacg ccgaggccga gttttatcgt
    15121 catggctacg cggcggtgca tcgcggtatt cataccttaa gcgcccaggc gaccgagaaa
    15181 atggagaacg tgcgcaagcg ggcatcgctg tttattaatg cccgttcggc ggaagagctg
    15241 gtgttcgtcc gcggcacgac ggaagggatc aatctggtcg ccaatagctg gggcaacagc
    15301 aacgtgcggg cgggcgataa catcatcata agtcagatgg agcaccacgc taacattgtt
    15361 ccctggcaga tgctttgcgc acgcgttggc gcagagctgc gtgtgatccc gctcaatccc
    15421 gacggtacgt tgcaactgga gacgctgcct acgctgtttg atgagaaaac tcgcctgctg
    15481 gcaattactc atgtctccaa cgtgcttggc acagaaaatc cactggcgga aatgatcacg
    15541 cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg ctcaggcggt gatgcatcat
    15601 ccggtggatg ttcaggcgct ggattgcgat ttttacgtgt tttccgggca taaactgtat
    15661 ggccccaccg ggattggcat tctttatgtc aaagaagcct tgctacagga gatgccgccg
    15721 tgggaagggg gcggttctat gatcgccacc gtcagcctga gtgaaggcac tacctggacc
    15781 aaagcaccgt ggcggtttga agccggtaca cccaataccg ggggcatcat tggtcttggc
    15841 gcggcgctgg agtatgtttc ggcgcagggg cttaataaca tagccgagta tgaacagaat
    15901 ctgatgcatt acgcgctatc acagctggaa tctgtaccgg atctcactct ctatggccca
    15961 caaaacaggc ttggcgttat tgcttttaat ctcggtaaac accacgccta tgatgttggc
    16021 agttttctcg ataattacgg cattgctgtg cgtaccggac atcactgcgc tatgccatta
    16081 atggcctatt ataacgtccc tgctatgtgt cgggcgtcgc tggccatgta taacacccat
    16141 gaagaagtgg atcgtctggt gaccggactg caacgtattc accgtctgct gggataacag
    16201 ggaggcacta tggctttatt gccggataaa gaaaagttgc tgcgtaattt tttacgctgc
    16261 gccaactggg aagagaaata tctctacatt attgagctgg gccagcgtct gccagaatta
    16321 cgcgacgaag acagaagccc acaaaatagc attcagggct gccagagtca ggtgtggatt
    16381 gtcatgcgtc agaatgcgca gggaattatt gaattgcagg gcgacagcga tgcggcgatt
    16441 gtgaaagggc ttattgcggt cgtctttatt ctctacgatc agatgacgcc gcaggatgtt
    16501 gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcac tcacccaaca tctcacccca
    16561 tctcgttcac aaggtctgga agcgatgatt cgcgcaattc gcgccaaagc cgctgcactt
    16621 agctaaacta aagggacagc tttcatccgg ttggctcaca ttgccggatg gtcaatgttt
    16681 acactgacct gcacaggagt gtttcggttt caggtgaaca taagcgccat ccgacgtttc
    16741 agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa cgcgcgtctt tgcttacact
    16801 cacgcttatc ggcgctttta gtgccatcca ggctgcctgg gcggttgatt atccgctacc
    16861 accaaccgga agccgactgg ttgggcaaaa tcaaacgtat acggtgcagg aaggggataa
    16921 aaaccttcag gccatcgccc gacgttttga tactgcggca atgttgatcc ttgaagccaa
    16981 taacactatc gccccggtgc caaaacccgg tacgacgata actattcctt cgcaactgtt
    17041 attacctgat gcgccgcgtc aggggattat cgttaacctt gcagagctgc gcctttatta
    17101 ttatccgccg ggagaaaata tcgtgcaggt ttatccgata ggtattggct tgcaggggct
    17161 ggaaacgcca gtgatggaaa cgcgtgtggg gcagaaaatc cctaacccaa cctggacgcc
    17221 tacggcaggc attcgtcaac gttcgctgga gcgtggcatt aaattaccgc cagtcgttcc
    17281 tgccggacca aataacccgc taggacgtta cgcactgcgc ctcgcgcatg gtaatggcga
    17341 atacctcatt catggcacca gtgcgccgga cagcgtcggt ttgcgcgtca gttcagggtg
    17401 tattcgcatg aatgcaccgg atattaaagc cttgttctcc agcgtgcgga cgggaacgcc
    17461 ggtgaaagtg atcaacgaac cagtgaaata ttccgtggag cctaacggga tgcgttatgt
    17521 tgaagtacat cgaccactat cggcagaaga acagcagaac gttcagacaa tgccatacac
    17581 actgccagca ggctttacgc aatttaaaga caataaggct gtagatcaga agttagtcga
    17641 taaagcgttg tatcgtcggg cagggtatcc ggttgcggtg agcagtggag caactcccac
    17701 agccagcaat gcgccttcag tagagtcagc gcagaatggt gaaccagagc aagggaatat
    17761 gttacgcgcg acgcagtagt ggtaaatagc agacaaaaaa atggcgcaca atgtgcgcca
    17821 tttttcactt cacaggtact attacttgcg gtatttagta gccatgttgt ccagacgctg
    17881 gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg cgttcacgtc
    17941 gttgctcagc tggtcaactt tagcgttcag agtctgaacg tcagaagaca gctgatcgat
    18001 tttagcgttg ctggagcaac ctgccagcag agtagaaccc aggattaccg cgcccagtac
    18061 cagtttagta gctttcatta ttaataccct ctagattgag ttaatctcca tgtagcgtta
    18121 caagtattac acaaagtttt ttatgttgag aatatttttt tgatgggaag gcacttattt
    18181 ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta gataaaaaaa ttgagagaaa
    18241 acaggatgct tccatcggat
//
LOCUS       DAZKFZ010000078        18119 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000078 DAZKFZ010000000
VERSION     DAZKFZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..2246
                     /locus_tag="ACHOQW_002464"
     CDS             <1..2246
                     /locus_tag="ACHOQW_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFP9573531.1"
                     /translation="LFFMTGNACATEWISSEDLITSDFHLMTADERNVVKAATDDSME
                     AAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYLSGNGHLIVLGNV
                     IVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVSDKATQFEVIKAE
                     FYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPDYDSVADNIVQGL
                     PLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTESLSPAVMLQLLQ
                     HPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADVNKKLMSVPVESV
                     QLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVADELINNEDPELRE
                     RITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTEDIERIAEQMYLD
                     NKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISYLLTQHDVPTVWD
                     ELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNGVVDEEMLNNAID
                     LLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVVSMKNSTRRQSER
                     GLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASLLTTLT
                     ESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVKTGATRKIRNEAR
                     HRLEEKQ"
     gene            2243..2692
                     /locus_tag="ACHOQW_002465"
     CDS             2243..2692
                     /locus_tag="ACHOQW_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573532.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(2777..3688)
                     /gene="rdgC"
                     /locus_tag="ACHOQW_002466"
     CDS             complement(2777..3688)
                     /gene="rdgC"
                     /locus_tag="ACHOQW_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP9573533.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3813..4721
                     /gene="mak"
                     /locus_tag="ACHOQW_002467"
     CDS             3813..4721
                     /gene="mak"
                     /locus_tag="ACHOQW_002467"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP9573534.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4864..6048)
                     /gene="araJ"
                     /locus_tag="ACHOQW_002468"
     CDS             complement(4864..6048)
                     /gene="araJ"
                     /locus_tag="ACHOQW_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP9573535.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(6174..9317)
                     /gene="sbcC"
                     /locus_tag="ACHOQW_002469"
     CDS             complement(6174..9317)
                     /gene="sbcC"
                     /locus_tag="ACHOQW_002469"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP9573536.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(9314..10516)
                     /gene="sbcD"
                     /locus_tag="ACHOQW_002470"
     CDS             complement(9314..10516)
                     /gene="sbcD"
                     /locus_tag="ACHOQW_002470"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP9573537.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            10706..11395
                     /gene="phoB"
                     /locus_tag="ACHOQW_002471"
     CDS             10706..11395
                     /gene="phoB"
                     /locus_tag="ACHOQW_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP9573538.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11453..12748
                     /gene="phoR"
                     /locus_tag="ACHOQW_002472"
     CDS             11453..12748
                     /gene="phoR"
                     /locus_tag="ACHOQW_002472"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP9573539.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            13155..14474
                     /gene="brnQ"
                     /locus_tag="ACHOQW_002473"
     CDS             13155..14474
                     /gene="brnQ"
                     /locus_tag="ACHOQW_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP9573540.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14550..15923
                     /gene="proY"
                     /locus_tag="ACHOQW_002474"
     CDS             14550..15923
                     /gene="proY"
                     /locus_tag="ACHOQW_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP9573541.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            16079..17896
                     /gene="malZ"
                     /locus_tag="ACHOQW_002475"
     CDS             16079..17896
                     /gene="malZ"
                     /locus_tag="ACHOQW_002475"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP9573542.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         4198 a         4467 c         4835 g         4619 t
ORIGIN      
        1 tcttattttt tatgactggc aatgcatgtg ccacagagtg gatcagtagc gaggacctta
       61 tcacctctga ctttcatctt atgacggcag atgagcgcaa cgtggttaaa gcggcaaccg
      121 acgatagtat ggaagctgct tatatgctaa aagataatat ccgctggtat tatcataatg
      181 gtgacctatc attacccgct aacttcagca ataaaaataa actggtggtg aacggtaatt
      241 taacgatttc cggcgattat gatgattatc tgtcaggtaa tggtcatctg attgtattag
      301 gtaatgttat tgtcgataac tttattaatc atgatttcgc gtatgtcaaa ggacagatga
      361 cagcgaaagg tttagtttat gctgattaca atgatcataa ctttgaagtg atgaagggaa
      421 ttagtgcgcg aggcattatt gtttccgata aagcaacgca atttgaagtc attaaagctg
      481 aattttatat caatgaagat ggaagtggtg aaggatataa ctgggatgag aatattcaaa
      541 aagcctattc actggtgact gccgatcttt atgatcacac tgaaatcgaa accgataaca
      601 tttccaatgc gtatcctgac tatgatagcg tcgcagacaa catcgttcag ggtttaccgc
      661 tttttcgaga taaagccgct cctgaaatta atgagaaatt aaaatggatt gaaacgggga
      721 aactcgataa tttcccagcg aataaaataa agcatcaaga tccactggtg gcacgttttc
      781 tgactcatac agagagttta tcacccgccg ttatgctgca actgttacag caccccgatg
      841 accagacgcg tgaatcgatg gcgcagtcct ggcccgctca acagatgcat tggcttaccg
      901 atgaattaat taaagatgag gctgttgcca gagggctggt taaaaacagt aatatttccg
      961 ccgatgtgaa taaaaaatta atgtctgtac ccgtcgagag cgtacagctg gagcaggcca
     1021 gacaggataa tctttctcca gatattgttg cgtcgttaag tcatagcccc tttctcagtg
     1081 tacgcaagac attactcagc cattacgatt acgcctggct ggttccgact gcagtggccg
     1141 atgagttgat aaataacgaa gacccggagt tacgtgaaag aattaccgga gcagatttaa
     1201 ccgcacaaca agcagtgatg ttgagtaaag ataaatcact gaaagttcgt gaagcattag
     1261 ctcggacgct tactgaatta aaaattaccc aactgtcagc aaccctgcgt acagaggata
     1321 ttgaacgtat cgcagaacaa atgtatctgg ataataaaga aaataaaaat atcgtaaagg
     1381 tgttattgat agcgttgccg gagatgcgtc aactgtctct ggcaaaagaa gatgtgcaca
     1441 atttgcgcga aggtgcgcgt tatctgacat cgaaggacgt gattagttat ctattgactc
     1501 agcatgatgt tcccaccgtc tgggatgagc tggcacgcga taaattattg ccgctggaat
     1561 ataagaaaca gttatggcaa cgcacattaa atttaatgat gagcaaacgg caagaagatc
     1621 aagagcaagc ctacgaagta cagctggcgc tgattgataa tggtgttgtt gacgaagaga
     1681 tgttaaataa tgccattgat ttactggttg atttacccgc tgaataccgc tatcgaatgc
     1741 gtaaccaatt atttgataat aaagaattac cttccggaat tatcaacaaa ctcgatcaac
     1801 agtatcgctt caactctgac tgggcgctgg ctgttgtcag tatgaaaaat agcacccgta
     1861 gacaaagtga aagaggatta caccgctgga accatgagga tagcgatatt tttgcggaac
     1921 tggcgacgat aaaagataaa tcagatgatg aatggtggcg tgcattgctg caaagtcgca
     1981 atgatcattt gcgccaaacg gcattacgca acgcacatac cccggcgtcg ttgttaacga
     2041 cattgactga gtctcaggat cggtcgctgg ctattaataa tccacagctg gctgccgatg
     2101 tgaaaacggt gtggctaaaa gaggatccat cattactttt atttgtcgat aaacccgatc
     2161 tttcgcagtt acgggattta gtgaaaaccg gggcaacgcg gaaaattcgc aatgaagcgc
     2221 gtcaccggct tgaggaaaaa caatgacaca acgcccctgg agcaagttac agcgggagat
     2281 ctatgactta ctgacgccaa cgatcaacct gcaaattcac tgtacccgtt atcccatgcg
     2341 cagccaaaat ggtggaagca ccgatttacc gcgttattgg atcacgttgg ataaaaacgt
     2401 gatctgggat tacccgaaag actttattgc aggcaacggt ggagtgcgta attttcacgg
     2461 tgaaacttgc tggtatcctt acctgacgga tatttgttca atttctgatt tattgcgtga
     2521 atatattgac accccaaaag cggagttgtt aacaaaacaa tttaccagcg ataaatgggg
     2581 gttagtgaat attctgcgtg ctgctgacag gcgaataggg atgaggcgat tagatcagtt
     2641 acggcgtaaa acgcataata ttgctgcgct aaaaaattat tgcccgccgt agcgaataat
     2701 tatatgcctg gtgtggcttc gtacgccgga taagacgcgg caggcgtcgc atccggcatt
     2761 aaaggaaaat cagcaattaa cgttgtgctt cgccacctaa tccttcaatc aggttttgaa
     2821 ttaacgctgc cagttcacca gtcatcagga tgaaatcggc atcaaaacgc tgggcgaaat
     2881 cttcacggtc gatatcttcg ttttgatcgc gcagctcgtc gcagaacttc agacgcttga
     2941 gcgaaccatc gtcgcacatc acaaactgaa tgcgctgctg ccagtcgagc gccagtttag
     3001 tcaccacttt tccggcttca atgtgattgg tgatctcttc gctggtcaga tcttgtttct
     3061 tcgcgcggat cactccgcca tcttccagca acgatttcag ctcggcttca tcaagcagct
     3121 ggaagccctg tgccgcacta ccggaacgta cccattcggt cagcgtcagt tcaatcgggt
     3181 ttgccatgct caatggtaca accggtaacg accccaggct tttacgcagc agtgccagcg
     3241 tatcttccgc ttttttggca ctggcgcagt ccaccataat caaaccgtta accgtgtcga
     3301 tccacatcat tgtctggcta aaacggctga aagcacgcgg caacagagag tgcagcactt
     3361 cgtctttcag cgaatctttt tcggttttct tcagcttacg cgcctgttcc gcttccagtt
     3421 tggcgatttt cgcttccagc gcctgtttaa tcaccggaga cggtaggatt ttttcttctt
     3481 tgcgcgcgca gataacaatt tgaccattgg caacgtgcgt taacgcatcg ctgtgcgatc
     3541 ccatcggagg aacccagccc atcttcgcca tgtcctggct gccgcatggg gtaaatgcca
     3601 tcgaggctag ctgtttttcc atctcttctg cacgcagcga aatctcgcgg ctaagacggt
     3661 aaaccattaa atttttgaac cacagcatga taatttccac ggccttgtcg ttaaatttag
     3721 cgggcatgat aacgaattgt cggcggcctt gcattgccaa tccggttgtc cgtctctacg
     3781 ctattgatat tgaaaaaaat aaggagagta ccgtgcgtat aggtatcgat ttaggcggca
     3841 ccaaaactga agtgattgca ctgggcgatg caggggagca gttgtaccgc catcgtctgc
     3901 ccacgccgcg tgatgattac cggcagacta ttgaaacgat cgccacgttg gttgatatgg
     3961 cggagcaggc gacggggcag cgcggaacgg taggtatggg cattcctggc tcaatttcgc
     4021 cttacaccgg tgtggtgaag aatgccaatt caacctggct caacggtcag ccattcgata
     4081 aagacttaag cgcgaggttg cagcgggaag tgcggctggc aaatgacgcc aactgtctgg
     4141 cggtttcaga agcagtagat ggcgcggcag cgggagcgca gacggtattt gccgtgatta
     4201 tcggcacggg atgcggcgcg ggcgtggcat tcaatgggcg ggcgcatatc ggcggcaatg
     4261 gcacggcagg tgagtgggga cacaatccgc taccgtggat ggacgaagac gaactgcgtt
     4321 atcgcgagga agtcccttgt tattgcggta aacaaggttg tattgaaacc tttatttcgg
     4381 gcacgggatt cgcgacggat tatcgtcgtt tgagcggaca tgcgctgaaa ggcagtgaaa
     4441 ttatccgcct ggttgaagaa agcgatccgg tagcggaact ggcattgcgt cgctacgagc
     4501 tgcggctggc aaaatcgctg gcacatgtcg tgaatattct cgatccggat gtgattgtcc
     4561 tggggggcgg gatgagcaat gtagaccgtt tatatcaaac ggttccgcag ttgattaaac
     4621 aatttgtctt cggcggcgaa tgtgaaacgc cggtgcgtaa ggcgaagcac ggtgattcca
     4681 gcggcgtacg cggcgctgcg tggttatggc cacaagagta aaaaacccag gcaattggcg
     4741 catcatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaaaatgt gccagaaccg
     4801 cgtagggcgg ataaggcgtt cacgccgcat ccggcaataa gcaacgcaca ccgtgacagt
     4861 aatctacccc agtggtttcg ccagcaccgg actatccgcc gcttgctggc gcttatagcg
     4921 accatacagc agcaacgatg acatcgcagc aaacgaaagc agggcggcag gcagcgccac
     4981 gtaattatat gccagcccca gcgtcagcat catacctccg caatatgcgc cgacggcgct
     5041 accgaggtta aacgctattt gcccacctgc ggcacctaat aactctccgc ctttggcatt
     5101 ttgcagtaac aatatttgca gaggtgctga aagggcaaat aatcccgcgc aacaaataaa
     5161 agcaaaaata agcgacgttg ttttcatgcc gccgcagaaa aagagcatca gcagtgccag
     5221 tacaattata aagtcagtca ctgctgcaat gcgcagtggt gaataacgtc ctgaaatcct
     5281 gccacttagc atatttccca gcaccatccc tagcccaact aacatcataa taaaggtcat
     5341 cgccgtttcc gaaaaaccgg aaataaacat catgtatggc tttacgtagc tgaaccaggc
     5401 aaacacacct gcgttgccaa acatggtggc ggcgaaaatt aaccacgggg ccgggctgcg
     5461 caaaaagtga aattgttcgc gcagctttcc tttcgcctcg tcgcgaatat ccggcaccca
     5521 aaaatagacc gatgccatca ccgcaatatt aaaaacagcg atcaataaaa aggtgtaacg
     5581 ccagctaaat tcctgactta aatacgttcc cagcggaatg cccagcaaat tggcgactgt
     5641 catcccggaa accatccccg ccacggcggc ggtgactttt ccgggtttga taatttttga
     5701 taacacgatc gctccgacgc caaaaaatgc gccatgcgga aagccggaaa ccaggcgacc
     5761 aatggcaagc atcaggtacg acgaagagag cgtgaacatg gcgttgccaa tgacgcacaa
     5821 cgccaccaga aacaacaaga tatgtttgag tgagtagcgg ctggaaaaga gtgcgattat
     5881 tggcgcaccg accaccaccc ccagcgcata atacgagatc atatgcccgg cagcaggaat
     5941 cgaaattcct acgttatgcg ccagctccgt gagcacgccc ataatgccaa attcggccat
     6001 ccccaaacca aacgtgccca gagccaaaga taaaatgact tttttcatac caccatcaca
     6061 actaccagaa cggctaccaa tcaccgtagc caataaagtg atagcttgca ggataacgac
     6121 cagcaaggcc aagagataat tcgcccctct gtattcatta tcctgctgaa tagttatttc
     6181 actgcaaacg tactttccag tttgctgtag cccaggccgt tgatcttctt cactttgatc
     6241 tgcacaggaa tacgctcttt catcgcttca acgtggctaa tcacaccgat ggttttgcca
     6301 ctggcgttca gggcatccag cgcatcaagg gcggtatcca gcgtttcgct atccagcgtg
     6361 ccaaaacctt catcaaggaa cagcgagtca atacgtgttt tatggctgac cagatccgaa
     6421 agcgccagcg ccagcgccag actaacgagg aaactttcgc cgccggaaag ggtacgggta
     6481 tcgcgtaccg catctgcctg ccaggtatca acaacctcga cttccagcgc ctcgctggct
     6541 ttgcgctgta acagatagcg cccgtgcagc cgggtaagtt gctgattagc gagatggact
     6601 aaattatcca gcgtcagccc ctgggcaaac ttgcggaatt tatcgccctc tttggaacct
     6661 attagcgaat tcagatatcc ccagtcctca acctgctgcg tcatttgagc aatttgctgc
     6721 agtaaggttt gttgttgctg acggttatct gcatcctgct tcagctgctg gcgaatctcg
     6781 ccttgactcg tggtgttttc acgcaacttt tggtgagttt gcgctaactc ttgctgaatc
     6841 tgctccaccg tcacagtgag agccaacccg tcaggtcggt gttgttgatg ctgtgccagc
     6901 gtttctgctg tctgagtgac cagcgtttgc gcctgacggc gctggttttc cagattctgc
     6961 ttgagctgtt ccagctgcgt tagtgtttgt tcatccatta gcgccgcaag gaacgcctgc
     7021 tgatcgtcaa agacgctggc ctgtagcgcg gtgtcaaact gcgcctgggc tttttgcaga
     7081 ctttgcgccg ccagaacatc ctgttgctgt aacgtctgct gctggctgtg taatgcgaga
     7141 cattgttcat gaacctgccg ccagttatcc agcgccacag tttcttcgct gtgcgggaga
     7201 tcatcacttt gcggcaacgt ttccagaatc ggcgtcagct gctgaatacg gttttgcagc
     7261 gcggttaatt cgttctggcg ttgctgccag ctctgcgctt cttgctgacg tgtcgccaac
     7321 cagctctctt cttcatcttc ctgtggcaat gtcagtgcat aacccgccaa tgccgttaag
     7381 agctgctgct ggcgttgttc aatttgctgt tgatactgga taatttgctg attatgcgcg
     7441 gcaatctgcc cttgtaattc atgtcgttgg ctgagtaacc gcagctggcg ttcgtgctca
     7501 tcttgtgcat ccagccacgg ttgaatatcg tcctgtggct gcaaggtgat attgaggctg
     7561 gccgtgacgg cttgccattg ttgagtaagt gcttgctcat cttgtcggag gctttgcgct
     7621 tcgttttcat cgcgctgaag ctgcttcgtt aatgcatcca gttgcccgcg cagcgccgca
     7681 ccttcttcgc cgagcttttt aacttcgttt tccagcgcca gtaatcgaga ctgattaacg
     7741 ccaggctcca gcgcctgata cgcctcgacc gccggatggc tggtggaacc acaaagtgga
     7801 caaggctgac ccgcctgtaa ctgtgcacgt tgagcttcca gcgttttgat gcgcgcttcc
     7861 tgctcgcaaa tggttttcac atcggcaagt tgctgcgtct tttctttata acgctggcgc
     7921 atttcgttaa gtgcggcatt acgttgcgtc tgttccagag tgacattctg gatagtgacc
     7981 attaactgcg ccagacgttt ttgttgggga acaatctgcc catgcagcgc gaccaggcgc
     8041 tgacgcagtg ggcgttgctc agcatgttgc gccagggcgg tagcaacttc atcggcggtt
     8101 aacatcaacg tgatcgccgc aagcgcatta agtttttgct cagcatgggt taactgttgc
     8161 tgccattgcc gcagatgctc gcgatcgctg gtttgttggg agaactgcgc acgccaaccc
     8221 gccagttcgt tgttccactg acggaagcgg tcgtgttcct gtaaccaggt attcaggctt
     8281 tgttgctgct gctgtaattc tgctgactgc ttcgccgcgt ggtggcgaat gctcgcgcga
     8341 agcgccattg tgttctgtaa gcgagtattt acttcttcaa tctgctggcg agtatgcgcc
     8401 agcgccgcgc tgtgttctgc gatgcgttcc cagtgcggac gaagatttcg tgccggttgt
     8461 gccagactga gcgccgccag ttgaggttgc gctttttctt cttcggctaa cgcctgttgc
     8521 aaggcctgct gacggcggct ggcttcttgc tgcaattcgt ccagacgcgt taaccagttt
     8581 agtgattgtt gttcttgctg ctgcgcggta agtaactgtt tttcttcgtc agtaagtacc
     8641 tgcaaactcg ctgtcagcga ttgcacttgt tccggcgtga gcaacgcgac gccgctggcc
     8701 tgcgcttgca gcttctccag ctctgtgcgg gccgatttgt gctgctcaaa aaccatcgcc
     8761 gagatttgcc cgtagatttc agtgccggtt aactcctcga gcaattccgc gcgttctttg
     8821 ggtttggcat tcaggaaggc agcaaattgc ccctgcgaaa gcagcatcga acgggtgaag
     8881 cgcccgtaat ccagcccggt taacgtcgct gtcagttcca gcttatcttt cactttgtcg
     8941 gcgagaattt tgccgtcggc gcagcgcgcc agctctacgc gtggcacctg caaattaccg
     9001 tcgggttggt tacgcgcccg attctggctc cagaacgcgc gatacgcttc acctttcact
     9061 tcaaactcca cctccgccag acattcagcg gtatcgcggg tcatgagatc attttgcgat
     9121 tgtgaaacgt tagagagacg cggagtttcg tgatacagcg ccagacaaat ggcgtccagc
     9181 agggtggttt tccccgcacc ggtcgggccg gtaatggcaa acagcccgtt gctggcgaac
     9241 ggctcgcggg tgaaatcaat cttccattcg ccttttaatg agttcaggtt tttcaggcgc
     9301 aggctgagaa ttttcatgct tcgtgttctc cggcgagggt atgcaacgtc gtggtgaaaa
     9361 gatgctgcag acgttgctgc tgcgattcat ccagttcttc cagtgccaga cggcgattga
     9421 acacctcttc gacgctgagt tcgctgaggg tttcacgctg ttggctggct aacacacgct
     9481 cgcgctgttc acgactccga cgtaccagca atacttcaac aggcaatgat tcggttaatg
     9541 cctggatttt gcgctgaata tcatgcagat actcatcagt agtgatttcg atatccagcc
     9601 agacaggtgg ctcctgcgat acatcgcgcc actgttccag ctgtgcggta atcgacgcca
     9661 gatcgccttt cagcactgcc atgggttgcg ttaccggtac gttcaggttt tccacgctct
     9721 ctaatttgcc gtttgaaaat gtcaccagat ggacatattt actcttaccg cattcatcaa
     9781 aactcagtgg aatgggggag ccgcaatagc gaacatgttc catgccgcca ataatctgtg
     9841 cgcggtgaat atgcccgagc gcgatgtagt cggctggtgg aaagttttgt gccggaaacg
     9901 cgtccagcgt gccaatataa atgtcacgca cggcgtcact tttactggcc cccacggtcg
     9961 ttaaatgtcc cgtggcgatg atgggcagag gctgatcgcc gcgcagtttg caggcatcgg
    10021 catagtgttg ttggtaataa tcggtaatcg ctgccagtaa atgctgctgt ttttcaatac
    10081 cgttaagccc cgcctggctg gtaataatgt cacgcggacg taaaaacgga atggggcaca
    10141 gcactgcgcc tggcgtcccg tcgcgacgag gcaagatttg cggcgcatgt ccggcgctgg
    10201 cgaccacggt agtattgagg aacgccatga tatcgcgcga ttcattcagc gtggcgaccg
    10261 agtcatggtt tcctgccagt accaccagat gacagccagt ttgctgtaaa ttgacaacaa
    10321 aacggttgta taacgtgcgg gcgtaactgg gcggcgagcc ggtatcgaaa acatcaccgg
    10381 caacaataat cgcatccacc tgatgggttt gtgctgtctc cagcagccag tcaagaaaag
    10441 cctgatgttc agcttcgcgg cttttactgt agaagttctg gccgagatgc cagtctgagg
    10501 tgtgaaggat gcgcataacg gttccctggc gaaaaagcat gggcgcgatt atacccaaac
    10561 agatgtgcca tttgcttttt tctgcgccac ggaaatcaat aacctgaaga tatgtgcgac
    10621 gagcttttca taaatctgtc ataaatctga cgcataatga cgtcgcatta atgatcgcaa
    10681 cctatttatt acaacagggc aaatcatggc gagacgtatt ctggtcgtag aagatgaagc
    10741 tccaattcgc gaaatggtct gcttcgtgct cgaacaaaat ggctttcagc cggtcgaagc
    10801 ggaagattat gacagtgctg tgaatcaact gaatgaaccc tggccggatt taattctcct
    10861 tgactggatg ttacctggcg gctccggtat ccagttcatc aaacacctca agcgcgagtc
    10921 gatgacccgg gatattccag tggtgatgtt gaccgccaga ggggaagaag aagatcgcgt
    10981 gcgcggcctt gaaaccggcg cggatgacta tatcaccaag ccgttttcgc cgaaggagct
    11041 ggtggcgcga atcaaagcgg taatgcgccg tatttcgcca atggcggtgg aagaggtgat
    11101 tgagatgcag ggattaagtc tcgacccgac atctcaccga gtgatggcgg gcgaagagcc
    11161 gctggagatg gggccgacag aatttaaact gctgcacttt tttatgacgc atcctgagcg
    11221 cgtgtacagc cgcgagcagc tgttaaacca cgtctgggga actaacgtgt atgtggaaga
    11281 ccgcacggtc gatgtccaca ttcgtcgcct gcgtaaagca ctggagcccg gcgggcatga
    11341 ccgcatggtg cagaccgtgc gcggtacagg atatcgtttt tcaacccgct tttaacgcct
    11401 tgctcatcgg acgcagagca gggcttatga tttcttaact ggagtatctt acgtgctgga
    11461 acggctgtcg tggaaaaggc tggtgctgga gctgctactt tgctgcctcc cggctttcat
    11521 cctgggtgca ttttttggtt acctgccctg gtttttgctg gcatcggtaa caggactgct
    11581 tatctggcat ttctggaatt tattgcgcct ttcatggtgg ctgtgggtgg atcgcagtat
    11641 gaccccgcca ccggggcgtg gtagctggga accgctacta tacggcttac accagatgca
    11701 gctgcgaaat aaaaaacgcc gccgtgaact gggcaatctg attaaacgct ttcgtagcgg
    11761 cgcggagtcg ctgcccgacg cggtggtgct gaccacggaa gagggcggta ttttctggtg
    11821 taacggtctg gcgcaacaga ttcttggttt gcgctggccg gaagataacg ggcagaacat
    11881 ccttaacctg ctgcgttacc cggagtttac gcaatatttg aaaacgcgtg atttttctcg
    11941 cccgctcaat ctggtgctca acaccgggcg gcatctggaa attcgcgtca tgccttatac
    12001 ccacaaacag ttgctgatgg tggcgcgtga tgtcacgcaa atgcatcaac tggaaggggc
    12061 gcggcgtaac ttttttgcca acgtgagcca tgagttacgt acgccattga ccgtgttaca
    12121 gggttacctg gagatgatgg atgagcagcc gctggaaggc gcggtacgcg aaaaagcgtt
    12181 gcacaccatg cgcgagcaga cccagcggat ggaagggctg gtgaagcagt tgctaacgct
    12241 gtcgaaaatt gaagccgcac cgacgcaatt gctcaatgaa aaggttgatg tgccgatgat
    12301 gctgcgcgtt gttgagcgcg aggctcagac tctgagtcag aaaaaacaga catttacctt
    12361 cgagatagat aacggcctca aggtgtctgg caacgaagat cagctacgca gtgtgatttc
    12421 gaacctggtc tataacgccg tgaatcatac gccggaaggc acgcatatca ccgtacgctg
    12481 gcagcgagtg ccgcacggtg ccgaatttag cgttgaagat aacggaccgg gcattgcacc
    12541 ggagcatatt ccgcgcctga ccgagcgttt ttatcgcgtt gataaagcgc gttcccggca
    12601 aaccggcggt agcggattag ggttagcgat cgtgaaacat gcggtgaatc atcacgaaag
    12661 tcgcctgaat attgagagta cagtaggaaa aggaacacgt ttcagttttg ttatcccgga
    12721 acgtttaatt gtcaaaaaca gcgattaatc cgcctttgtc atcttttatt gccataagcc
    12781 agccgatgct ggcttatttt ctttgcagtc aaaatacggg cgttagattt tacaacggtt
    12841 ggtgattttt tgttcgcatg attagccatg tctttttcac ggaaatagtg ttttatactg
    12901 gttggtgatt tcttatcgct atatacctct ggtttttaga tccctccttg ctttaaaacg
    12961 ttataagcgt ttaaattgcg cttcaggtgc tgtcatactg actgcattaa cgcggtaaat
    13021 cgaaaaacta ttcttcgccg cgcctggttg ggagtatttc ccgctaaaat tgtttaaata
    13081 taccgctgta tcatccccag ggattggcac aaaaatttaa cgttacaaca ccacatccac
    13141 aggcagtatg atttatgacc catcaattaa gatcgcgcga tatcatcgct ctgggcttta
    13201 tgacatttgc gttgttcgtc ggcgcaggta acattatttt ccctccaatg gtcggcttac
    13261 aggcaggcga acacgtctgg actgcggcat tcggcttcct cattactgcc gttggcctgc
    13321 cggtattaac ggtagtggcg ctggcaaaag ttggcggcgg tgttgacagc ctcagcacgc
    13381 caatcggtaa agtcgctggc gtactgctgg caacggtttg ttacctggcg gtggggccgc
    13441 ttttcgctac gccgcgtaca gctaccgttt cttttgaagt ggggattgcg ccgctgacgg
    13501 gtgattccgc gctgccgctg tttatctaca gcctggtcta tttcgctatc gttattctgg
    13561 tttcgctcta tccgggcaag ctgctggata ccgtgggcaa cttccttgcg ccgctgaaaa
    13621 ttatcgcgct ggtcatcctg tctgttgccg cgattgtctg gccggcgggt tctatcagca
    13681 cggcgactga ggcttatcaa aacgctgcgt tttctaacgg cttcgtcaac ggctatctga
    13741 ccatggatac gctgggcgca atggtgtttg gtatcgttat tgttaacgcg gcgcgttctc
    13801 gtggcgttac cgaagcgcgc ctactgaccc gttataccgt ctgggctggc ctgatggcgg
    13861 gtgttggtct gactctgctg tacctggcgc tgttccgtct gggttcagac agcgcgtcgc
    13921 tggtcgatca gtctgcaaac ggcgctgcta ttctgcatgc ttacgttcag cacacctttg
    13981 gcggcggcgg tagcttcctg ctggcggcgt taatcttcat cgcctgcctg gtaacggcag
    14041 ttggcctgac ctgtgcttgt gcagaattct ttgcccagta cgtaccgctc tcttatcgta
    14101 cgctggtgtt tatcctcggc ggcttctcga tggtggtttc taacctcggc ttaagccagc
    14161 tgatccagat ctccgtaccg gtgctgaccg ctatttatcc gccgtgtatc gcactggttg
    14221 tattaagttt tacacgctca tggtggcata attcgtcccg cgtgattgct ccgccgatgt
    14281 ttatcagcct gctttttggt attctcgacg ggatcaaagc atctgcattc agcgatatct
    14341 taccgtcctg ggcgcagcgt ttaccgctgg ccgaacaagg tctggcgtgg ttaatgccaa
    14401 cagtggtgat ggtggttctg gccattatct gggatcgcgc ggcaggtcgt caggtgacct
    14461 ccagcgctca ctaaatcact gaacatttgt tttaaccacg gggctgcgat gccccgtggt
    14521 tttttattgt gttgatgggt taggaattga tggaaagtaa gaacaagcta aagcgtgggc
    14581 taagtacccg ccacatacgc tttatggcac tgggttcagc aattggcacc gggctgtttt
    14641 acggttcggc agacgccatc aaaatggccg gtccgagcgt gttgttggcc tatattatcg
    14701 gtggtatcgc ggcgtatatc attatgcgtg cgctggggga aatgtcggta cataacccgg
    14761 ccgccagctc tttctcgcgt tatgcgcagg aaaacctcgg cccgctggca ggttacatta
    14821 ccggctggac ctactgcttt gaaatcctta ttgtcgccat cgccgatgtg accgcttttg
    14881 gtatctatat gggtgtctgg ttcccgacgg tgccgcactg gatttgggta ctgagcgtgg
    14941 tgctgatcat ttgcgccgta aacctgatga gcgtgaaggt attcggtgag ctggaattct
    15001 ggttctcgtt ctttaaagtc gccaccatca tcatcatgat tgtcgccggt ttcggcatca
    15061 tcatctgggg gattggcaac ggcgggcaac cgaccggtat tcataacctg tggagcaacg
    15121 gcggcttctt cagtaacggc tggcttggca tggtaatgtc gttgcaaatg gtgatgtttg
    15181 cttacggtgg gatcgaaatt atcgggatta ccgccggtga agcgaaagat cctgagaaat
    15241 cgataccgcg tgcgattaac tccgtgccga tgcgtattct ggtgttctac gtcggtacgc
    15301 tgttcgtcat tatgtctatc tacccgtgga atcaggttgg cactgccggt agcccgttcg
    15361 tgctgacgtt ccagcatatg ggcattacct ttgccgccag cattcttaac tttgttgtgc
    15421 tgactgcttc gctgtcggca attaacagtg atgtatttgg cgtaggccgt atgctccacg
    15481 gtatggcaga gcagggcagc gcgccgaaaa ttttcagcaa aacgtcgcgt cgcggtattc
    15541 cgtgggttac ggtgctggtg atgactaccg cgctgctgtt tgcggtgtat ctgaactaca
    15601 tcatgccgga aaacgtcttc ctggtgatcg cttcgctggc aaccttcgcc acggtgtggg
    15661 tgtggattat gatcctgctg tcgcaaattg ccttccgtcg ccgtttgccg ccagaagaag
    15721 ttaaggcgct gaaatttaaa gtgccgggtg gggtagcaac gaccatcggc gggctgattt
    15781 tcctgctctt tattatcggg ttgattggtt atcacccgga tacgcgtatc tcgctgtatg
    15841 tcggtttcgc gtggattgtt gtgctgttga ttggctggat gtttaagcgt cgccacgatc
    15901 gtcagctggc tgaaaaccaa taatacttgc gttctgtaag ccggttcagg aggaaatcct
    15961 gatccggcta ttccgaaagt tatccgcccc cgtcctcctc ccccaaatat ccttcagatg
    16021 atgagtgatc ctgcattagg ctatggcaag gtgatcagat tttcatcaca ggggaattat
    16081 gatgttaaat gcatggcacc tgccggtgcc cccatttgtt aaacaaagca aagatcaact
    16141 gctcattaca ctgtggctga cgggcgaaga cccaccgcag cgcattatgc tgcgtacaga
    16201 acacgataac gaagaaatgt cagtaccaat gcataagcag cgcagtcagc cgcagccagg
    16261 cgtcaccgca tggcgtgcgg cgattgatct ctccagcgga caaccccggc ggcgttacag
    16321 tttcaaactg ctgtggcacg atcgccagcg ttggtttaca ccgcagggct tcagccgaat
    16381 gccgccggca cgactggagc agtttgccgt cgatgtaccg gatatcggcc cacaatgggc
    16441 tgcggatcag attttttatc agatcttccc tgatcgtttt gcgcgtagtc ttcctcgtga
    16501 agctgaacag gatcatgtct attaccatca tgcagccgga caagagatca tcttgcgtga
    16561 ctgggatgaa ccggtcacgg cgcaggcggg cggatcaacg ttctatggcg gcgatctgga
    16621 cgggataagc gaaaaactgc cgtatctgaa aaagcttggc gtgacggcgc tgtatctcaa
    16681 tccggtgttt aaagctccca gcgtacataa atacgatacc gaggattatc gccatgtcga
    16741 tccgcagttt ggcggtgatg gggcgttgct gcgcttacgc cacaatacgc agcagctggg
    16801 aatgcggctg gtgctggatg gcgtgtttaa ccacagtggc gattcccatg cctggtttga
    16861 caggcacaat cgtggcacgg ggggagcttg tcacaacccc gaatcgccct ggcgcgactg
    16921 gtactcgttt agtgatgatg gcacggcgct cgactggctt ggctatgcca gcttgccgaa
    16981 gctggattat cagtcggaaa gtctggtgaa tgaaatttat cgcggggaag acagtattgt
    17041 ccgccactgg ctgaaagcgc cgtggaatat ggacggctgg cggctggatg tggtgcatat
    17101 gctgggggaa gcgggtgggg cgcgcaataa tttgcagcac gttgctggga tcaccgaagc
    17161 ggcgaaagaa acccagccgg aagcgtatat agttggagag cattttggcg atgcacggca
    17221 atggttacag gccgatgtgg aagatgccgc catgaactat cgcggcttca cattcccgtt
    17281 gtggggattt cttgccaata ccgacatctc ttacgatccg cagcaaattg acgctcaaac
    17341 ctgtatggcc tggatggata attaccgcgc agggctttct catcaacagc aattacgtat
    17401 gtttaatcag ctcgacagcc acgatactgc gcgatttaaa acgctgctcg gtcgggaggt
    17461 tgcgcgcctg cccctggcag tggtctggct gttcacctgg cctggtgtac cgtgcattta
    17521 ttacggtgat gaagtgggac tggatggcaa aaatgatccg ttttgccgta agccgttccc
    17581 ctggcaggtg gaaaaacagg atatggcgtt attcgcgttg taccagcgaa tgattgcgct
    17641 gcgtaagaaa agtcaggcgc tgcgtcgtgg cggctgtcag gtgctgtatg cggaagataa
    17701 cgtggtggta tttgtccgcg tgctgaatca gcagcgtgta ctggtggcaa ttaaccgtgg
    17761 cgaagcctgt gaagtggtgc tacccgcgtc accgtttctt aatgtcgtgc aatggcaacg
    17821 caaagaaggc catgggcaac tgacagacgg gattctggct ttgcctgcca tttcggctac
    17881 ggtgtggatg aactaaagca tctgttttat ttgttacgtc gttagtcacc agccctgaca
    17941 ctacgcagcg caaaaaagct gctcaatgtc agggctgaat ttgttaaata tttgtcccga
    18001 tcgatatgag cgatttttgg tattgtttgc ccatgatgtt atgtacaaca tgctccagga
    18061 taaccctgta gtggagcaaa gatatgacaa tttcaaattc gaatttattg aaaccgttt
//
LOCUS       DAZKFZ010000079        17454 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000079 DAZKFZ010000000
VERSION     DAZKFZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            67..321
                     /gene="bssS"
                     /locus_tag="ACHOQW_002476"
     CDS             67..321
                     /gene="bssS"
                     /locus_tag="ACHOQW_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP9573543.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            436..1554
                     /gene="solA"
                     /locus_tag="ACHOQW_002477"
     CDS             436..1554
                     /gene="solA"
                     /locus_tag="ACHOQW_002477"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP9573544.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            1602..1715
                     /locus_tag="ACHOQW_002478"
     CDS             1602..1715
                     /locus_tag="ACHOQW_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP9573545.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            1976..2542
                     /gene="yceJ"
                     /locus_tag="ACHOQW_002479"
     CDS             1976..2542
                     /gene="yceJ"
                     /locus_tag="ACHOQW_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9573546.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2546..3121
                     /gene="yceI"
                     /locus_tag="ACHOQW_002480"
     CDS             2546..3121
                     /gene="yceI"
                     /locus_tag="ACHOQW_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP9573547.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3163..4215)
                     /gene="trhO"
                     /locus_tag="ACHOQW_002481"
     CDS             complement(3163..4215)
                     /gene="trhO"
                     /locus_tag="ACHOQW_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9573548.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4440..5360
                     /gene="lpxL"
                     /locus_tag="ACHOQW_002482"
     CDS             4440..5360
                     /gene="lpxL"
                     /locus_tag="ACHOQW_002482"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP9573549.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5532..6758
                     /gene="mdtG"
                     /locus_tag="ACHOQW_002483"
                     /pseudo
     CDS             5532..6758
                     /gene="mdtG"
                     /locus_tag="ACHOQW_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            6841..7215
                     /gene="msyB"
                     /locus_tag="ACHOQW_002484"
     CDS             6841..7215
                     /gene="msyB"
                     /locus_tag="ACHOQW_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9573550.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7216..7443)
                     /gene="yceK"
                     /locus_tag="ACHOQW_002485"
     CDS             complement(7216..7443)
                     /gene="yceK"
                     /locus_tag="ACHOQW_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9573551.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7616..10159)
                     /gene="mdoH"
                     /locus_tag="ACHOQW_002486"
     CDS             complement(7616..10159)
                     /gene="mdoH"
                     /locus_tag="ACHOQW_002486"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP9573552.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10152..11687)
                     /gene="mdoG"
                     /locus_tag="ACHOQW_002487"
     CDS             complement(10152..11687)
                     /gene="mdoG"
                     /locus_tag="ACHOQW_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP9573553.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12080..13237
                     /gene="mdoC"
                     /locus_tag="ACHOQW_002488"
     CDS             12080..13237
                     /gene="mdoC"
                     /locus_tag="ACHOQW_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP9573554.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13245..14726)
                     /gene="clsC"
                     /locus_tag="ACHOQW_002489"
     CDS             complement(13245..14726)
                     /gene="clsC"
                     /locus_tag="ACHOQW_002489"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP9573555.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14668..15201)
                     /gene="ymdB"
                     /locus_tag="ACHOQW_002490"
     CDS             complement(14668..15201)
                     /gene="ymdB"
                     /locus_tag="ACHOQW_002490"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP9573556.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15296..15607)
                     /gene="ymdA"
                     /locus_tag="ACHOQW_002491"
     CDS             complement(15296..15607)
                     /gene="ymdA"
                     /locus_tag="ACHOQW_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP9573557.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15728..16060)
                     /gene="csgC"
                     /locus_tag="ACHOQW_002492"
     CDS             complement(15728..16060)
                     /gene="csgC"
                     /locus_tag="ACHOQW_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP9573558.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16119..16577)
                     /gene="csgA"
                     /locus_tag="ACHOQW_002493"
     CDS             complement(16119..16577)
                     /gene="csgA"
                     /locus_tag="ACHOQW_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP9573559.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(16618..17073)
                     /gene="csgB"
                     /locus_tag="ACHOQW_002494"
     CDS             complement(16618..17073)
                     /gene="csgB"
                     /locus_tag="ACHOQW_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP9573560.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4389 a         4424 c         4127 g         4514 t
ORIGIN      
        1 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
       61 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
      121 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
      181 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
      241 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta
      301 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
      361 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
      421 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
      481 gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc ttatgaccga cgcccatatg
      541 ccaccacatc aacacggcag ccaccacggc gatacgcgat taattcgaca tgcttatggt
      601 gaaggcgaaa agtatgtccc gctggtcctc cgcgcacaaa cgctgtggga tgacctctcc
      661 cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct tggcccggct
      721 gactccgcat ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa
      781 aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
      841 atcggcttat ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg
      901 atccaactgg cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt
      961 cgtcatgacg atgatggcgt aactattgaa acggctgacg gtgagtatca ggcgaaaaaa
     1021 gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc
     1081 gtacgtaaag tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc
     1141 ccggcgttta ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac
     1201 gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
     1261 gttccgtttg cggaagtggt cagcgatggt tcggaagcct tcccgttctt gcgcaatgta
     1321 ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac
     1381 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
     1441 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
     1501 gacaaaaaaa gcgattttga tttgacgcca ttcagccttt cccgcttcca ataatcatca
     1561 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact
     1621 atctcatcaa taatattcgc gagcatctga tgctatatct ttttctctgg ggattgttgg
     1681 caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca
     1741 aatatttaat ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac
     1801 agcactcacc agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat
     1861 taattcaaaa ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa
     1921 ttcattctcc gacacgccat tttctttctc tcacaaattc gttttggatg cacttatgtc
     1981 attcacaaat acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc
     2041 aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga
     2101 tggctggtat cacaaagcac ccgaactaca taaaagcatc ggtattttgt taatgatggg
     2161 gctggttatt cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta
     2221 ttcgcccatg actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt
     2281 cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt
     2341 tggctggttt gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg
     2401 tgccctgcat ttttggctcg cgtggagcgt cgtagtactg tccgttatgc acggatttat
     2461 ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc
     2521 gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc
     2581 cctgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca
     2641 gcacgccttt gttaactttc gcatccagca tcttggctat agctggttat atggcacctt
     2701 taaagatttc gacggtactt ttacctttga tgaaaaaaat ccagctgccg ataaagtgaa
     2761 tgtgacaatt aacaccacca gcgtcgatac taatcacgcc gaacgtgata aacatcttcg
     2821 cagtgcagat ttcctcaata ccgcaaaata tccacaggca acattcacct ccaccagcgt
     2881 gaagaaagac ggtgacgaac tggatattac cggcgatctg acgctgaatg gtgtaaccaa
     2941 acccgtcacg ctggaagcga aattaattgg tcagggcgac gacccatggg gtggtaaacg
     3001 tgcaggcttc gaggccgaag gcaaaattaa gctcaaggac ttcaatatca agacagattt
     3061 aggtccagct tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta
     3121 agagtgaaat gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg
     3181 aatgcccagt gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc
     3241 acgtcctgcc cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat
     3301 ttcactacaa caacctttgt atttttccgc acatactgga cactgaataa acagcaggtg
     3361 gcagccatca tttttacagt tggtatggct gtcgcacggc gcaccgcact ggtggcaatg
     3421 cgcgataatc tcatcagata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc
     3481 aataaaacgc accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccacc
     3541 ctcgatatgc cagactttat tgaatccgtt atgtttcatc caggcactgg ccttttcaca
     3601 acgaatgccg ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc
     3661 gactgctttt ggcagctgct cacggaaggt atctgccgga atttccagcg cgttttcaaa
     3721 gtgccccact tcatactcat agtggttacg catgtcgata aacagtgcat cgggatcgtc
     3781 aagcatggcg ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg
     3841 aggatcgtca ataccgtcgg caacgatacg atcgcggacc ttcatgcgca gtacccagaa
     3901 ggatttcccg tcatcatcca acgcgatatt caggcgtaag ccatctaaag ccgagtcgaa
     3961 ggcatagagc tgcgcgcgaa atgttttaac attgctcgca ggtacgctga tttgcgcgtt
     4021 aatgccctca tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata
     4081 taaagcgtca cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt
     4141 ggttcgcggt tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt
     4201 gtgtaacact ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg
     4261 atatagcaat tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa
     4321 ttatttgtta tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc
     4381 gggcgaaaaa atgcgacaat acatacaatt gcccgaatag gttgaaaaac aggattgata
     4441 tgacgaatct acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt
     4501 tgggtattgg cgtactttgg ttagtcgtgc aattgcccta cccggttatc taccgcctcg
     4561 gttgtggatt aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc
     4621 gcaacctgga actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga
     4681 agaatttcga atccgttggc atgggactga tggaaactgg catggcgtgg ttctggccgg
     4741 accgccgaat cgctcgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg
     4801 cgcaaaaacg cggcatcctg ttagttggca tccattttct gacactggag ctgggtgcgc
     4861 ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga
     4921 ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag
     4981 atttaaaagg catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc
     5041 atgattacgg cccgcgctca agcgttttcg tccctttatt tgccgttgag caggctgcga
     5101 ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc
     5161 cacgccgtaa gccagatggc aaagggtatc aattgattat gctgccgcca gagtgttctc
     5221 cgccgctgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca
     5281 tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag
     5341 gcgttccttc acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta
     5401 ccacagcaaa tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc
     5461 gggcgcataa ttagtgtgct tatcttttct tcttatgttc accgcgcctg gcgcaccaac
     5521 agcggattgc tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga
     5581 tcgtcgcctg gctaggctgt tttcttaccg gtgccgcctt cagtctggta atgcccttct
     5641 aacccctcta cgttgagcag cttggcgtta ccggccactc cgccctgaat atgtggtccg
     5701 gtattgtctt cagcattaca tttttatttt cggccatcgc ctcaccgttt tggggtggac
     5761 tcgccgaccg taaaggccga aaactcatgc tattacgctc tgctctcggc atgggcatcg
     5821 tgatggtgtt gatggggctg gcacaaaata tctggcagtt tttgatcctg cgggcgcttc
     5881 ttgggttact tggcggattt gtccccaacg ctaatgctct tatcgccaca caagtaccgc
     5941 gtaataaaag cggctgggca ctgggtacgc tctccacagg cggcgttagc ggtgcgttgc
     6001 tcggcccaat ggctggcggc ctgctcgccg atagctacgg cttacgtccg gtattcttta
     6061 ttaccgccag tgtgctcata ctctgctttt tcgtcaccct gttttgcatc agagaaaaat
     6121 tccagccggt cagcaaaaaa gagatgctgc acatgcggga agtggtgaca tcacttaaaa
     6181 acccgaaact ggtactcagc ctgtttgtca ccacgttaat catccaggtg gcgacgggct
     6241 caattgcccc cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg
     6301 cctttatcag tggcatgatc gcctcggtgc caggcgtagc tgctctgctg agtgcaccac
     6361 gactcggcaa acttggcgat cgaatcggac ccgaaaagat cctgattacg gcgctgatct
     6421 tttctgtact gctgttgatc ccaatgtctt acgttcagac gccattgcaa cttgggattt
     6481 tacgtttttt gctcggtgcc gccgatggtg cactactccc cgccgtacag acactgttgg
     6541 tttacaactc gagcaaccaa atcgccggac gtatcttcag ctataaccaa tcgtttcgtg
     6601 atattggcaa cgttaccgga ccattgatgg gagcagcgat ttcagcgaac tacggtttca
     6661 gagcggtatt tctcgtcacc gctggcgtag tgttattcaa cgcagtctat tcatggaaca
     6721 gtctacgtcg tcgtcgaata ccccaggtat cgaactgatt tttcgccttt catacttgca
     6781 aaagcggaga atcagctatc cttttccctg aaacctcatc aactcaaagg gagaatcgtg
     6841 atgaccatgt acgcaacgct tgaagaagcc attgacgctg cacgcgaaga atttcttgca
     6901 gacaaccccg gcatcgacgc cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac
     6961 gttttgcagg acggcgacat catgtggcaa gttgagtttt ttgccgacga aggggaagaa
     7021 ggtgaatgtt tacctatgct tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat
     7081 gatgagatag agatccgcca ggagtggcag gaagagaata cattacatga atgggacgag
     7141 ggggaatttc agcttgagcc accgctggat accgaggaag gacgcgcagc agctgatgag
     7201 tgggatgaac gttaattact catacgggcc atgatgaatg tcgatcggca gcagtaaagt
     7261 atcaaaaacc agggagaatg gcagatcaag gattgtgaca taacgccagg cggagtcacg
     7321 cacatcccat tgcacaccgg gataatattg gttgccatgc ccctgccccg gtatagtgcg
     7381 gctaatgatg ctgccacagc cactcagcaa ggtcaccatg atgctcacca caattaaacg
     7441 catttgatac ctcttccgta aaaaaatacc ggcataacgc cggtattttt attggctcgt
     7501 tgtttttgcc agatgtggcg tgaacgcctt atccgaccta caaaatcttg caaaatcaac
     7561 acattgcagg agcgatgtag gcctgataag catagcgcat caggcaacta cgtttttatt
     7621 gcgaagccgc atccggttta cgcaatgcca gtggattgag ctttatccct tcgtaataac
     7681 tcacccatga agaatatctc tcaggggaat tccagacgcg gaaatgcaga cgggccatcg
     7741 tcaccggatc gcttagcagc acaaggcgac gatcgcgatt cagcttctct ggcgtctcgt
     7801 tcagcgcctg ttcaacatgg cggtcacggg cgatttccag caccttactg gcgcggtgac
     7861 gcgcggtcgc cattgcggtt gccagagcgt taaatgacgg gttaaacact gcgtgcataa
     7921 aaccatcatc aagggagcgt tgacggttca tctcaaggaa ccgatcggta tcaaccagta
     7981 cctgcggcgg tgaatactct tccgggatca ggaacagttt ccagcgtttg gtgcgcagac
     8041 caacggtggc acggctggaa atcaccgaaa caaacggtga caggatcaac gagaagacaa
     8101 tcggtgccag ccagaacagg aaacgcagat ccaaccacgc catcccgaca gcccacacta
     8161 accctaacag cagctgtgag ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat
     8221 catcacgctg cggtgaattc cacaccactt cccagccaag gaacgcgctg acgacgaaga
     8281 ccgtatggaa cagcatgcgt accggagcca gcagcacgga aaacagcact tccagcagca
     8341 acgataatgt aacgcgccag aagccgccat attctttcgt tcctttacac caaataagca
     8401 aaatgctcaa tagctttggc agaaacaaca gcaccatggt cgaagcaaaa agtgcaatcg
     8461 ccagctcagg acgccactgc ggccacaccg ggaacaactg ccgtggttgc aggaagtatt
     8521 gcggttcggt caacgcatgc actacctgca atgcagtaga gagcgcgagg aacataaacc
     8581 acagcggagc ggagagataa gacatcacgc ccgtcaggaa taccgcacgg tgaaccgggt
     8641 gcataccctt caccaggaac agacggaagt tcatcaggtt accgtggcac cagcggcggt
     8701 cacgttttag ctcatcaagc aagttaggcg gtaattcttc ataagaaccc gggagatcgt
     8761 aagcaatcca gactccccaa cctgcacggc gcatcaacgc cgcttccacg aagtcatgtg
     8821 acaggattga accggcaaag gaaccttcgc ccggcagcgg ggccagtgca cagtgctcga
     8881 taaacggttt cacgcggata atcgcgttat gcccccagta gtgcgactcg ccaagttgcc
     8941 agaagtgcaa accggctgta aacagtggcc catacacgcg ggtcgcgaac tgctgacagc
     9001 gtgcatacag cgtatccata ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg
     9061 ggttggcttc catcaggcgc accaggccgc acaaacaatc accggtcatt accgagtcag
     9121 catccagcac caccatatag ctgtactggc tgccccagcg acggcagaag tcatcgatat
     9181 taccgctttt acgcttcacg cgacggcggc ggcggcgata gaaaatctga ccttcgccac
     9241 cgacttcagc gataagctcc atccaggctt tttgctctgc gacgcagata tccgggttgt
     9301 aactgtcact cagaatgtag acatcaaagt gtttggcatt cccggtggct tttactgatt
     9361 cccacgttgc acgcaagcca gcaaaaacac ggttcacgtc ttcgttacag ataggcatga
     9421 tcaacgccgt gcgatgctcc gggtttaatg gttcatcgcc aactgttgac gcagatatac
     9481 tgtatttatc gcgaccaata agtagttgca ggaagcccat taacgccgtc cagaatccgg
     9541 cggacaccca acagaacagt accgcaaaga ggatcaggat accggtttgc agcatataag
     9601 gcagaagctg cataaaggaa acccacaaat cctgaccaac catatccata ggattaatca
     9661 gcgcccaacc ctgataagga agaatggtct tcatatacca ggtcgcgacg acagtttgcg
     9721 cgagcgtcag gatcaacaga atgtaacggc ggatggtacc gacggtacgc cacttttgct
     9781 cgctctcctg ctcttctttg gtcaaacgag ccagatagcg cggtgttaca tcgcgtccac
     9841 gcaggcgatc ccagaaacgg cctaccgggt tggtacgcca cgggtcggga aacatcgagg
     9901 agcgttttgc ttctggcatc gccttaagct gatcgcgccc ttcgtcatct ttaattaact
     9961 gtccatcagc aagtgaatct ggccaggctt gttccagacg cgcctttacc gagccttgcg
    10021 gggagtcatc ctcccgcgcc caggtgcggt gttcggcatc cagcgcctga tgaacggcgc
    10081 ggatatcagt cttcggcaat gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt
    10141 actcagttgt cttattcatt ggcaggtaac tggtagctcc aggtttcact caacgtctga
    10201 tcggcattca ccagcgcagc gcgcatttca gtggttttct tggcatcttt cactttcaca
    10261 cgcatcacca gacgccagcc tttggtgacc gggttatagc gcaccgtgct ttcaactatc
    10321 tcaccattat caccaatgct ggtttgcgct gtgaccgggg tatcctctgg cagttttttc
    10381 atctctgcgc cggtaaaatc gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg
    10441 ttcgactgct tcacatcccc cgttgaacga cgcgtttgtt gcacccatgc gttatctggc
    10501 gcatgcagtt tgtcttcatc acggctgaag gtgatggtgt atttaaagtt catctcttta
    10561 cccggctccg gcagctgatc cggcgtccag taagcgacga tgttatcgtt agtttcatcg
    10621 ttggttggaa tttccaccag ctcaacgctg cctttacccc actccccttt cggagtcacc
    10681 catgcgcttg gacggagatc gtaacgatca tcgagatctt caaagcggga gaaatcacga
    10741 ccgcgctgca acagaccaaa gccttgcggg ttttccatgg agaagctgct gaccgctaaa
    10801 tgtttcgggt tattcaacgg acgccagatc cattcgccat taccggcatg gatagagaga
    10861 ccgttagagt cgtgcaactc cggacgatag ttatttgctg gcgacggttg gttcggccca
    10921 aacaggaaca tactggttaa cggtgcaacc cccagtttgc cgactttatc gcgcagatag
    10981 attttcgact gcacatccac aaccgtgtca cgccctggca taactacgaa tttgtaagca
    11041 cctgtcgcgc gcggcgagtc aagcaatgca taaatggtta aacgtttatc agttggtttt
    11101 ggacgctcga tccagaactc tttgaagcgt ggaaattctt cacccgatgg caaggcggta
    11161 tcaattgcca gaccgcgggc agaaaggcca taaacctgac ctgcaccaat cacgcggaaa
    11221 tagctggccc cgagcatgct gacgatttca tcgtttttat ctttgctgtt gatcgggtaa
    11281 agcactttaa aaccggcaaa accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg
    11341 ccgaaagtga aataatccgg gctgtatttg attcgtttga ctgcggtggc agtcacttca
    11401 tttattttga ccggggtatc gaagtacata ccctgatggt agaactcgag tttgaatggg
    11461 gtcttcagat tgttccagta cgctttgtca tgattaaact ggatctgctg atagtccgcg
    11521 tatttcatat cgcggaaaac ggagggcaag ttgcttttgg gcgcctcata gcctttcccg
    11581 gctaaggatt gagcttgctt tgcgacatca tcaatactga aagcccagct tgaagatgta
    11641 tacagggtta acattactgc agcactcaac caacgcattt tcatcatttg tagtttatgt
    11701 ttcataatta gtaagcactt ccccctttgt gtgcttatat cgatccgatc tattttaatg
    11761 gataaccagc cattccgaca acagaatccc ggtatcgttc agcacgctgg ctcatggatt
    11821 aagcacctga ttgcaaccgt tttcactttc cgtgcttctc ctaaagacag aagcatcgac
    11881 ttcgtgtagg gtttgccagc aaatttattt atcaaccgtc ttacagacgc ttccattctg
    11941 gttagtggta gcgatgatgc tatgcataaa aacttgaagc ctgcgcctgg cgcggtcagg
    12001 tcggtgtcgc tatggcaaca agcctaaagg cataacccga cagggataag acgaaaaatc
    12061 cgagattagt taaccatata tgaatccagt acccgcgcaa cgtgaatatt tcctcgactc
    12121 catccgcgcc tggctgatgt tgttagggat cccttttcat atttctttaa tctattcgag
    12181 ccatacatgg catgtgaata gcgccgaacc gtcattgtgg ctgacccttt ttaatgactt
    12241 catccactcg ttccgcatgc aggtattttt cgttatatcc gggtactttt cctacatgct
    12301 ttttttacgc tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg tagggatccc
    12361 gatgttaaca gccatccccc tactgacatt gccgcaattt attatgctgc aatacgtcaa
    12421 agggaaagcg gaaagttggc ctgggctgtc attgtatgac aaatataata cgttggcctg
    12481 ggaattaata tcacatctgt ggtttttact ggtgttagtg gtcatgacga cgctgtgcgt
    12541 atggatattt aaacgcatca gaaataattt agaaaattct gataaaacga ataaaaaatt
    12601 ctcgatggta aaactatcgg tgattttttt gtgcctcggc atcggttatg cggtaataag
    12661 aagaacgatt tttattgtgt atccacccat tctgagtaat ggcacgttca attttattgt
    12721 catgcaaacg ctattttatt taccgttctt tatcctcggc gcactggctt tcattttccc
    12781 tcatcttaaa gccttgttta ccacgccgtc tcgtggctgt accctcgctg cagcattggc
    12841 gtttgtcgct tatttactca accagcgcta tggcagtggc gatgcctgga tgtacgaaac
    12901 cgagtcggtg atcaccatgg tcctcggtct gtggatggtg aatgtggtct tctcctttgg
    12961 ccaccgtttg cttaacttcc agtcagcgcg ggtgacttat tttgttaacg catcgctgtt
    13021 tatctatctg gttcaccacc cgttaacgct gtttttcggc gcatacatta caccgcacat
    13081 cacctccaac tggcttggtt ttctctgtgg cctgatattc gtagtaggga ttgcgataat
    13141 tctgtatgaa attcatttgc gcatcccgtt actgaagttt ttgttctctg gtaaaccggt
    13201 tgttaagcgt gagaacgata aagcaccagc ccgttaagcc acatttacaa taaccattcc
    13261 acgggcaata tcgacgccag tctgaccata acccgcttcc agaaactggt agcgggttct
    13321 tttttgagaa caatctcttt ctttgcatga cgatcaaccc agttgatccg tccccacctg
    13381 tccagacgga gctgccaggc cgcatcatac tggctctgaa taaagcgttt atcaattaac
    13441 tgagccagcg tttcgctctc tatcacgaag cccatttcag tattgagcaa tgttgaacgc
    13501 ggatcgaaat tgaaagaacc gataaacacc gttttaccat cgatgctaaa ggttttagca
    13561 tgcaggctgg caccggaatt accggttatg ccgcgatcgt gtaacgtact actttgttca
    13621 cgcgtcggct tgagttcata taattccacg ccatagcgga gcaatttttt gcgccagcgc
    13681 gcgtatccgg catggacgac agcaacatcg ttagcggcaa gagaattggt taggatcgca
    13741 atctttaccc cttttctcac catccgtaag agttgcgcca cacctgcgcg tgtcggtaca
    13801 aaataggaag agataatatc gatgcgttca ctgggtgagc ccatgatatc gaacaggcgc
    13861 tgcggtaaca gtgaatgccg ttttgccttg ccctcccctt tcgccggatc atcacttaat
    13921 aaacgtgttt tcgcccagat aagcggcaac gttccatcaa ccagatgatt tataaacgga
    13981 ctggattcca ttttgcgtaa gtaacgatgc gtcatggcat cgttatgcca ggaggcgggt
    14041 aactcgatgc gatccgccat ttcaccttcc gggacatcca gcacctgctg taagggtgaa
    14101 accgatttgc aataccagta gcgggcgaaa tcatcggcaa cgtcctctac tacgggtcct
    14161 attgccatga catctaaatc cgaaaaaagt ggctcttccc ctgccccaaa ataggcatca
    14221 ccaatatttc gcccccctac caacgtcacc acgccatcga cagtgaaact tttattgtgc
    14281 atacggcgat tgagacggga aaagtcggtg atataaccaa gcggacgcag caagcgaaac
    14341 gagaaaggat taaaaagccg gacttcaatg cggggatggc tgtcaagcaa gcgtaaaatg
    14401 tcgtcaagtc cgggcgtatt gttgtcgtcc agcaacaaac ggacgcgaac gccacgcttt
    14461 gctgcggcta acagggcgga aaacagtaac cgacccgaca tatcgtcctg ccagatgtaa
    14521 tactgaacat ccagcgtatg ctctgacatt tcggccagac gataccgggc ggcaaacgca
    14581 tccagacttt tctccagagg gaaaagacca cactgaccgg gatgttgcga acacagtggc
    14641 agcaccgcgc tcgccagccg gggcaaatca ttcatctcct tgttgggtaa ggagtctttc
    14701 gtagaggtgg gcgttttctt catcataaca gacaaagtat acctgttcgg gtaaagcgtg
    14761 acgggtaata aattctgaaa cggtttttac tgcgatttca gccgctgccg cacgagggta
    14821 accataaacc ccagtactga ttgcaggaaa agccactgac gtatagctgt ttgccgccac
    14881 cagtcgtagg ctattgagat aggcatcctg caaaagctgg tcttcgtttt gttcaccacc
    14941 acgccagact ggcccgacgg tgtgcactac ggctttagca ggaagatcgc ctgcaagcgt
    15001 aataacggca tgccccgtag ggcaatcgcc ctgctgttgc ctgactttta aacaagcatc
    15061 cagcagggcc ggacccgctg cgcgatgaat ggccccatcg acgccgccac ctcccattaa
    15121 tgaaggatta gccgcattca caatcacatc aacggccagt ttggtaatat caccctgcac
    15181 aacatgaata cgcgttttca taacgcctcc ttacaccttc ttgtttctta agtgtacagc
    15241 aggctttttg gttaaatcgc atttttctgc tcagcaacgt gagttgttca gaaacttagt
    15301 tatattcgat gttcattaca gccagctttt tctgtttatc aagatactgc acttttactg
    15361 aggctatttg cgcgtttttg acattgccgc tcattaacgc tttgctggag taggttttac
    15421 ccggaacaga accattctgt gggcagttca tttctacggg tgatgactgg gtgctgacgt
    15481 cacatgccgg ttccacaatt tggccataaa aatggatcac gcccccttgc gcggtgcttc
    15541 ctgcgatggc aataaaagga aaaaacaaac tgccgagcat aagagagttt aaaaaaggtc
    15601 tgaacattta ttctatttcc tcaatgatta gtcatccttg agggttgtgt tatccatact
    15661 ttcaggttgg ctgattcgca gcagaccatt ctctccagat tcatcttatg ctcgatattt
    15721 caacaaatta agacttttct gaagagggcg gccattgttg tgataaatga agtgactgtc
    15781 catcagaaac agtaacaact attttcaccc gatcgtccgg ggaaatattt aaactcaact
    15841 tcgtcaaagc aatgggttga ttagcaggca atgaaagggt cttttcttgc ttcgtctgac
    15901 tttgccctga actgccttcg cgcagggaca atatttgtac tctgcacaga caagattgag
    15961 taagagtgac ttcaggaata atggtgtaca tatccccttg ctgggtcgta ttaaaggtta
    16021 tctgactgga aagtgccgca aggagtaata acgcattcat attcttctcc cgaaaaaaaa
    16081 cagggcttgc gccctgtttc tttaatacag atgatgtatt agtactgatg agcggtcgcg
    16141 ttgttaccaa agccaacctg agttacgttg acggtggaat tagatgcagt ctggtcaacc
    16201 gctgcaccgt tgccgccacc gaattgttta actgtcatat gagagtcttt accgttccac
    16261 tgatcaagag tggcgctgtt accaaagcca cgttgggtca gatcgattga gctgtcatct
    16321 gagccctgac caacatctgc accgttacca ccaccatgct gggtaatagt aagatcagag
    16381 ttacgagcat cagcttgcag agcaagtgca gagttaccac caccgtactg ataaatattc
    16441 agctctgaat tcgggccgct gttattaccg ccaccaccgt ggttaccgcc accgccgccg
    16501 tactgaggaa caacacctgc cagagcgcta ccggagaata cgattgctgc aattgctgct
    16561 acttttaaaa gtttcatgta aaacccccat cggattgatt taaaagtcga atggaaatta
    16621 acgttgtgtc acgcgaatag ccatttgcga ctgtctctgc actacaattg ccgttttttg
    16681 agtaccatac tgtgtaatat ttgctttatt accagaacct ttctggataa tcatcgcagt
    16741 attaccataa gcaccttgcg aaatactggc atcattggca ctgcccgcct gatcaatata
    16801 tgcaaggtta taatctcctg tctggtcaat ctttgcccgg ttgctactac cttcttgcgc
    16861 aacaaccgcc aaaagttttg agcctccctg ccgtaactga gcactattat tagtcccagc
    16921 ttgaccaatt atggctgcct gattaaatga agacttactc aattcattta ccgcgaagtt
    16981 atattctgaa ttagctaaat cataacctgc tgcggctgca atcccaggcg cacccagtat
    17041 tgttaacatc ataaataaca atttgttttt catgttgtca ccctggacct ggtcgtacat
    17101 ttaagaaatt aaatcatttc aacttggttg ttaacgcaac ctatattttg ttaacgctgc
    17161 gttacgatgg aaagtatgtc tgcggaaata tttttaataa ctcacccgcg cgttgtattt
    17221 ttcttttttt gttcatacca aagtattaaa acccacgtaa atacgctgtt atctacgcaa
    17281 aaaatatttt gttttctttt aaatctccgt tttccgctag caaaaaacac cagacagtca
    17341 ttcttcttgc ccgtcgctga ttgctgcgat atgtcttgcg cacaagccgt gacaaacctc
    17401 gtctttttgc agtaaaaaat tgtccacggt ggtgtggaga aaaaacaaga acgt
//
LOCUS       DAZKFZ010000080        17442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000080 DAZKFZ010000000
VERSION     DAZKFZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(120..1148)
                     /gene="epmB"
                     /locus_tag="ACHOQW_002495"
     CDS             complement(120..1148)
                     /gene="epmB"
                     /locus_tag="ACHOQW_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9573561.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1190..1756
                     /gene="efp"
                     /locus_tag="ACHOQW_002496"
     CDS             1190..1756
                     /gene="efp"
                     /locus_tag="ACHOQW_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9573562.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1808..1933
                     /gene="ecnA"
                     /locus_tag="ACHOQW_002497"
     CDS             1808..1933
                     /gene="ecnA"
                     /locus_tag="ACHOQW_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9573563.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2044..2190
                     /gene="ecnB"
                     /locus_tag="ACHOQW_002498"
     CDS             2044..2190
                     /gene="ecnB"
                     /locus_tag="ACHOQW_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9573564.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2366..2683
                     /gene="sugE"
                     /locus_tag="ACHOQW_002499"
     CDS             2366..2683
                     /gene="sugE"
                     /locus_tag="ACHOQW_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9573565.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2680..3213)
                     /gene="blc"
                     /locus_tag="ACHOQW_002500"
     CDS             complement(2680..3213)
                     /gene="blc"
                     /locus_tag="ACHOQW_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9573566.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3301..4434)
                     /locus_tag="ACHOQW_002501"
     CDS             complement(3301..4434)
                     /locus_tag="ACHOQW_002501"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9573567.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4497..4856)
                     /gene="frdD"
                     /locus_tag="ACHOQW_002502"
     CDS             complement(4497..4856)
                     /gene="frdD"
                     /locus_tag="ACHOQW_002502"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9573568.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4867..5262)
                     /gene="frdC"
                     /locus_tag="ACHOQW_002503"
     CDS             complement(4867..5262)
                     /gene="frdC"
                     /locus_tag="ACHOQW_002503"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9573569.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5273..6007)
                     /gene="frdB"
                     /locus_tag="ACHOQW_002504"
     CDS             complement(5273..6007)
                     /gene="frdB"
                     /locus_tag="ACHOQW_002504"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9573570.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6000..7808)
                     /gene="frdA"
                     /locus_tag="ACHOQW_002505"
     CDS             complement(6000..7808)
                     /gene="frdA"
                     /locus_tag="ACHOQW_002505"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9573571.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8133..9110
                     /gene="epmA"
                     /locus_tag="ACHOQW_002506"
     CDS             8133..9110
                     /gene="epmA"
                     /locus_tag="ACHOQW_002506"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9573572.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9329..10831
                     /gene="yjeM"
                     /locus_tag="ACHOQW_002507"
     CDS             9329..10831
                     /gene="yjeM"
                     /locus_tag="ACHOQW_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP9573573.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(10982..14305)
                     /gene="mscM"
                     /locus_tag="ACHOQW_002508"
     CDS             complement(10982..14305)
                     /gene="mscM"
                     /locus_tag="ACHOQW_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP9573574.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14327..15295)
                     /gene="asd"
                     /locus_tag="ACHOQW_002509"
     CDS             complement(14327..15295)
                     /gene="asd"
                     /locus_tag="ACHOQW_002509"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP9573575.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15392..16444)
                     /gene="rsgA"
                     /locus_tag="ACHOQW_002510"
     CDS             complement(15392..16444)
                     /gene="rsgA"
                     /locus_tag="ACHOQW_002510"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP9573576.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16539..17084
                     /gene="orn"
                     /locus_tag="ACHOQW_002511"
     CDS             16539..17084
                     /gene="orn"
                     /locus_tag="ACHOQW_002511"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP9573577.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17295..17370
                     /locus_tag="ACHOQW_002512"
     tRNA            17295..17370
                     /locus_tag="ACHOQW_002512"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17328..17330,aa:Gly,seq:gcc)
BASE COUNT         4027 a         4508 c         4589 g         4318 t
ORIGIN      
        1 ataaacagaa aagaaaggtt aattattggt gttggttata taaagagtaa caacagcaat
       61 gtatatgaat aattaaaggt tatgtaacgg gcaaagatta aacttccctg tttaatcagt
      121 tactgctggc gtagctggag atccagcggc gttttgctgg gttcgccgcc aatttctcgc
      181 gccagtttcg gcaccagata tcccgacacc agtgtcagca actcacgcat aatctgccgt
      241 gcttcgtcat cactcaccat aaaatgcgcc gcgccctgta ctttatcgag cacatgcagg
      301 taatacggca tcacgcctgc atcgaacaac gcattactca ggtttgccag cgtttgtgcg
      361 ttatcgttca caccacgtaa cagaacgctc tggttcagca aagtaacgcc cgcccggcgc
      421 aacttagcca tcgcctgacg gaatgtttca tctatctcat tggcatggtt gatgtgattc
      481 accagcaaga tttgcagcgt agaatgtgaa aagcgttcaa ccagcgcatc tgtgatgcgt
      541 gccgggatca caatcggcag acgactgtga atccgcagac gtttgatatg cgggatggct
      601 tccagttgtg tgagcaacca gtccagctcg tgatctttcg ccatcagcgg atcgccgccg
      661 gagaaaatca tctcgtccag ttccggatgc gcagcaacat actcaagtgc agtttgccag
      721 ttacgcttgt tgccctgatt ttcggcatag gggaagtggc gacggaagca atagcggcaa
      781 tttaccgcgc agccgccttt gaccagcaaa agcgcccggt tgtggtattt atgcaacaaa
      841 ccaggcacta cgctgtgctg ttcttccagc gggtcggtgg agaatccggg cgcgacgaca
      901 aactcatctt gcgaggtaag tacctgacgc aaaagcggat cgtccggatt gcctttctcc
      961 atgcgatcga taaatgagcg gggcacacgc agggcaaaca gctttttggc gttgcgtccg
     1021 gccaacagtt tttcgtccgc gtctatattc aaaagacgca gaagttcatc aggatcggtc
     1081 acaacatcgg caagttgcgt taaccaatct tctctggatg gggtatttag ggttacaata
     1141 tgcgccattt tgtggcttag ctaccaatta acaaatttca gagggcctta tggcaacgta
     1201 ctatagcaac gattttcgtg ctggtcttaa aatcatgtta gacggcgaac cttacgcggt
     1261 tgaagcgagt gaattcgtaa aaccgggtaa aggccaggca tttgctcgcg ttaaactgcg
     1321 tcgtctgctg accggtactc gcgtagaaaa aaccttcaaa tctactgatt ccgctgaagg
     1381 cgctgatgtt gtcgatatga acctgactta cctgtacaac gacggtgagt tctggcactt
     1441 catgaacaac gaaactttcg agcagctgtc tgctgacgca aaagcaattg gcgacaacgc
     1501 taaatggctg ctggatcagg cagagtgtat cgtaactctg tggaatggtc agccgatctc
     1561 cgttactccg ccgaacttcg ttgaactgga aatcgttgat accgatccgg gcctgaaagg
     1621 tgataccgca ggtactggcg gcaaaccggc taccctgtct actggcgctg tggttaaagt
     1681 tccgctgttc gtacaaatcg gcgaagtcat caaagtggat acccgctctg gtgaatacgt
     1741 ctctcgcgtg aagtaatgcg gttgtggtgc ggcctgcagg ctgcaccatc acttattcag
     1801 gtcagagatg atgaaacgcc ttatcgttct tgttttgctt gccagcacgc tgctcacggg
     1861 ctgtaacacc gctcgcggtt tcggcgaaga catcaaacat ctcggcaact ccatctcccg
     1921 cgctgccagc taatttttct tctcttccga aaaatcatca gatttccatc atttttggcg
     1981 atgttgtcta ttattaattt gctataggca aacataaata acattaccta aaaggaagac
     2041 gttatggtga agaagacaat tgcagcgatc ttttctgttc tggtgctttc aacagtatta
     2101 actgcctgca acaccacgcg tggcgttggt gaagacattt ctgatggcgg taacgcgatt
     2161 tctggtgcag caacgaaagc gcagcaataa gcaataacgg tacgacagct gtgtcgtgcc
     2221 gtttgttttt tctgcgatag tcacaaaggt aatagttgaa attcccccgc cacctggcaa
     2281 aatatccgtt caaccatcag ctttgcagga cgacctgcaa acgcgtcttt tcaccgggga
     2341 cggccccaat tctccggagc ctgatatgtc ctggattatc ttagttattg ctggtctgct
     2401 ggaagtggta tgggccgtag gcctgaaata tacccacggc tttagtcgtt tgacgccgag
     2461 tgttattacc gtgacggcga tgattgtcag tatggcgcta cttgcctggg cgatgaaatc
     2521 gttaccagta gggacggctt atgccgtgtg gacgggtatt ggcgcagtcg gcgcggccat
     2581 caccggcatt gtgctgctcg gtgagtccgc taacccgatg cgtctggcga gtctggcgtt
     2641 aatcgtattg gggattattg gtctgaaact cagcactcac taactaccgg gctgctgtac
     2701 ccaaataaac ttactgacat caaacccttc ccgggtcgcg actgccagca tctcctgttt
     2761 cacttcgtca gaaatggttg gcgtgcggga gagtatccac aggtagtcgc ggtccggtcc
     2821 gcaaaccagc gcatggcggt attcccgatc gagtgcaata acgttataac cgccatagaa
     2881 aggaccaaag aatgacactt tcagcgcagc gcgagttggt gcgccggtaa agtacgcttt
     2941 cccttcactc tgctgccaca ttcctctgtc agggttatag cctttattaa tgacattcag
     3001 gccgccgtca tcacgcaggc tgtatgttgc ggtgactttt tccagtccac gttcaaagcg
     3061 gtgatcaaaa cgggcaatct cataccaggt accgagatag cgtttagcgt cgaaattatt
     3121 cactacggtc acgccacgcg gcggcgtagg agaactgcag gcaacgacca gaaatgcagc
     3181 tgtcgctgcg gcaacgagag ggagcaggcg cataaatgtt tccttactgg ttttttctaa
     3241 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3301 ttactgtaga gcgttgagaa tctgccaggc ggcggcgact ctcgctggat tgggatagtt
     3361 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3421 aaatccgccg gtcgcccccg ttttatgcac ccatgatgct cgtactgcag gagttggagg
     3481 cgtaatcgct tttacggggc gtgctgccag tgcaatttta ttgtcactgc cgttaacgat
     3541 gatgtcagga tttaccggcc agtccagcat ttcccagccc agaccctgat acatatcgcc
     3601 ggtttgccag tagcgagatt gtgccagttg tatcccttgc tgaagtgttt tgtcgttgat
     3661 atcacgggga ttcatattgc ttcgtaccca gcaggccata tcttcaatgg tcgacttcac
     3721 accataagtt tcagcatcta acgcccctgg cgaaacatga actgccttgc cttcgcgata
     3781 tccccaggcg taattctttt cttctgcggg cggtacatta atccacgtat ggttgagttt
     3841 gagtggctgg aagacacgag tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     3901 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggagc
     3961 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacttcatc
     4021 cggcacctgc aatggcaggc cgccagcagt gtaggttgcg agatgtaata gtgtgatccc
     4081 attccactgt ttagcggtaa gttcaggcca gtattttgtt gcgggatcgc ttaacttgat
     4141 ttcccctcga gcaatagcgt cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4201 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg cataccccca
     4261 ggtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4321 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4381 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acatagggtc
     4441 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatgcgttag
     4501 attgtaacga caccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4561 catttgcccg caggtacgtg gattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4621 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4681 gcgaacgcca gaacgcgctc gtagctcagt gcatcacccg gaaacagccc cagcggcagc
     4741 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4801 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     4861 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     4921 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     4981 cgctttcggt gccagttcaa accaggtttt ggtgtgcagc agagctgccg ccagagtgat
     5041 caggttaatg atcacaataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5101 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5161 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5221 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5281 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgt tgaatggccg
     5341 cagccggatc gacgtgtttc gggcagactt cggagcagta gcccacgaaa gtacagctcc
     5401 atacgccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5461 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5521 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5581 acttcgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5641 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5701 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt cataccgtcg gtgtaatcac
     5761 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     5821 aaatcgccat acggcaggac cagcggtagc tcaggtccgg tgccaggttg tctttgatgt
     5881 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     5941 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6001 cagccattcg ccttctcctt cttattggct gcttccgcct tatcggctgc atccgcttcg
     6061 ccaccgtaaa cgcgtttagc tggcggtagc gtagtaatct tcacgtcgct gtactccagg
     6121 cgagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgaggaagtt gacgtcgtca
     6181 cgcttggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6241 gcggagtgcg ccatacattc agcaacgttc agaccgtggc ccagttcaat ggtgtagagc
     6301 aggtcggtgt tgaacacgct ggaagtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6361 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6421 ccttcttcca tagccaggcc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6481 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6541 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6601 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6661 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6721 tcgataccgc ccatggtgta gtgtgcggtc ggacgtaccg gaatcggttc tttaaccgga
     6781 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     6841 ttctcgccga ggtgacgcag gtcgagataa accacatcac cacgcggcgt ggagatggtg
     6901 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     6961 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7021 taacggtagc catttttgtt gaccagaata ccgccttcac cgcggcaacc ttcggtcatc
     7081 aggataccgg aacctggcag accggttgga tgatactgaa cgaattccat gtcacgcagc
     7141 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7201 ttgtaacgat aaacgcgacc cgcaccgcca gtagccataa cgaccgcgtt agcacggatc
     7261 tgcaccagcg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7321 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7381 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtacgc
     7441 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7501 gggcaccccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7561 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7621 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7681 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7741 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7801 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     7861 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     7921 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     7981 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8041 tgcccaccgt tgttttattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8101 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8161 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8221 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8281 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8341 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gccggttgcg
     8401 ggccggtatt ccagctgtgc cgcagtttcc gtaatgaaga gatggggcgt tatcacaacc
     8461 ctgagttcac tatgcttgag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8521 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8581 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8641 gggaagtggc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8701 cgttgctaca attgctgttt acctttggcg tagaaccaaa tattggtaaa gaaaaaccga
     8761 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     8821 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     8881 atgaattgac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     8941 cggcgcgcgg tttgccgcag caccccattg atcagaatct gattgaagcc ttgaaagtcg
     9001 gtatgcctga ctgttccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9061 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9121 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9181 ttttccgcta aaggccacaa tttaagactg gatatttgat atcatccagg tatcaattct
     9241 gtattggttt ttacgctgcc cgctcaatgt tgggcagtgc agttcagtgg gcgcgtttta
     9301 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9361 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9421 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9481 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggcggtat
     9541 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9601 gttttcctct tatatcatct ggatggtgag cacctccgcg aaagtttggg taccgttctc
     9661 aacattcctc tatggtagcg acatgaccca gcactggcgt attgccggac tggagcctac
     9721 gcaggtggtt ggtctgctgg cagtggcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9781 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
     9841 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
     9901 ggatattaat ttccttgcat caccgaaccc aggttatcag tccggtctgg caatgctatc
     9961 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10021 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10081 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10141 attaagtaat ggttccgtta acctcggcaa tattacctat gtgctgatga agagcctcgg
    10201 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10261 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10321 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10381 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgct atgtggatgc agtgcgggtt
    10441 ggttactgtc ttcatcctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10501 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10561 tttcccattc tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg
    10621 tatgtcggca atgattgcga cggtggttgt cgttctggtg gtgacatttg cgaacgtctt
    10681 caccattatt cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg
    10741 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10801 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    10861 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    10921 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    10981 attacaaact tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt
    11041 taccgttgag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11101 catggaagcc agccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11161 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga
    11221 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11281 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac accacgcgcg
    11341 tgaccgagtc agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca
    11401 cgattatctc tttacggtcc cagtcgctga tggtggttgc gcgggtatta attttcgtca
    11461 cgctaccagt aagatcgcga attgtcaccg tatcgccaat gcgaatcggt ttttcgaaca
    11521 ggatgatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
    11581 caccgagcgc ggcgaccagc cactgcaatt tcgaccactc aataccaatc atcgagaagc
    11641 cgaccagccc gccaatcagc atcagcagat atttggtgat ggtggtgatg gcgtagccgg
    11701 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcagattgc
    11761 gcaccagctg cgtggtgatg ataaacacca gaatggcaat cagcaccgca ccgagggtaa
    11821 tcggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    11881 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa agcagggcga
    11941 tcaacatcaa aattgagcgc actagccgca aggactgcgc actgatggca tcgagatcga
    12001 cttcgctttc atcgacttca attgcccctt ccgggctact gtgatgatgt gcttcctctt
    12061 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12121 gcctgcgtcg ttggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12181 accagatggc aaccgaggtt tcaagcctcg ctaacagcgc ctgtgccgtt gccagataac
    12241 ccaccgccga cgccagaatg gcaaccagtg gcgcgccaat catcatgttc cacaacatat
    12301 ggttggtaat attgtcgccg ctgccttctt tgttgagata cagcgggatc ccggcctttt
    12361 tcaggctgag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12421 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagtg
    12481 gcacaataag cccgatgctc atcaggtagt agcgcatccc acgagaaaca cgttcgcgcg
    12541 gccagccaaa atgagcgata aacaagccgt tcgggcgggc aaaggtagcg caaatcatca
    12601 ctacccacag cagcggcacg gtggctgtga cgccatcacc aatcgcgacc gccagcggat
    12661 aaggccacgc ctcgcgcaag ccgtaaccca gcgtcatcca cagcaccggc agcggtgacg
    12721 cgacgagaat cgaccagaaa agagtgcgca acgtcagcca gaagtgatcc tgagtcactt
    12781 tgccgacttt cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac
    12841 tgcaaccgac cagaatcaac gcgccaaaca gcggcaaaat cgtctctttg ctggtcagca
    12901 tcatcacact ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat
    12961 cctgggcgat ttccagcggc caggcgatgg tcatcgggcg cacgtcagag gtccagaaca
    13021 gatagcggtg ggtcgcttcg ttcacttctt tcagcgcatc ttccagttgc ccgttggaga
    13081 ctttcagctt ggtcagttcc agcagtagcg tatcgccacc ctgcaacaat gagttcagca
    13141 actcacgctg agtgcgtagc tgtgcttcca gaatacggtt ttgctcagcg gtcagcggct
    13201 gaccgtcggc ctgatgaatt tgccgtagca gcggctgttt attaagcaga tcctcataac
    13261 gtaaccgttg cacacgcaac tgcgccattt cggtatcaag ctgttgtggt ttcggcattt
    13321 ccggcagccg tgccacctgc gcccgcaacg cttcaccgag cagattggac gatcccagcc
    13381 attgcgactg ttcacgcagc gtattcaacg cctgccggac ctgtaacgtc tggctggcag
    13441 cctgacgctg ttgcgaggca acgagatcca tccgctgcgc ctgttgattc agcgccgccg
    13501 atagttcgcg gttaattttg aattgcgcga cgatatcttt cggcaaatcg gcactgtttt
    13561 ctgctagtaa ttcggtactt tccagcgccc gctctgcctc aagctggcgt tggctgttca
    13621 attgattacg caaggcctgc aaatacgcat ccagttgctg gctctctttt tcagccagct
    13681 cagagcgcaa gcgcgctaat tcctggcggt tattggcaga cagctgcgcc agctccagtt
    13741 catcaacgag cgccttaaga cgtgcagagt cagactgcaa cgcgaaattt tgtgcctgat
    13801 tgagcggagt attgccggta agcgtcccca ggcggcgctc gatctcattt aactgacggc
    13861 gggcgtcggt ttgctgttgc ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct
    13921 cctgctcttg ctgggcctga cggcttttat ccagcaactg actgctgacc tggagaattt
    13981 cctgattcag cgcgtcggta gacatccccg gcgacacgct gcgcggctcg tcacgcatgt
    14041 tgtttaattg tgcgcgcaga gtagcggaga gtttcggata attatcaatg acttcttgat
    14101 attgcttgat gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcagactgga
    14161 gtgcctctac gacttctggc tgcgcgggtt tcgccgcttt tgcctgctcc agttcctggg
    14221 tgatttgttt gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca
    14281 tcagaaaagt gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt
    14341 ttttatcgtc aaccaatggg ctggcgtcgt gttctgcttc gatctcttca gcaggaagcg
    14401 gggcgggttc agcgtctggc gtaacaaagg tttcggtaga tactgccagc ggctggccaa
    14461 ttttcgtgac agacaggctt tccagttgct caaccagatt cactttaccc ggtgcaaaca
    14521 ggttgataac ggtagagcca agtttaaagc gacccatttc ctggcctttc agcagcgcca
    14581 cagaaccgtc gttttccccg gcaggccagg tccagcgctt gatgatgcct tcgcgcggcg
    14641 gcgtaatggt gcccgcccag accgtctcaa tgctgcctac aatcgtcgcc ccgaccagaa
    14701 tctgcgccat tgggccaaat tcggtatcga acaggcagat cacgcgttcg ttacgggcaa
    14761 acagattcgg cacgttctga gccgtgagat ggttaacgga gaagagatcg cccggcacgt
    14821 agatcatctc acgcagaata ccgttgcacg gcatgtgtac gcggtggtag tcacgcgggg
    14881 agaggtaagt ggtcacaaac gtaccgttgc ggaacaggtc cgccatcaga tagttgcctg
    14941 ccagcagggc ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga
    15001 ttttacccaa ctggctgata acgccatcgg caggcatgac caggacattc ggatcggtat
    15061 cgattgggcg tacttcgtca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc
    15121 tggcggtgtc cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat
    15181 cgataaccag ttttgtcagc catcctgccc gcttgcttgc gccccaaccc gccaggcgag
    15241 taagccatag tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag
    15301 cctccaggcc attgttttgt cgttcctgat ccggcctaaa tgccggatcc tgaaaaaaag
    15361 gggacgattc taacgacggt tagcttaatt gtcagtcatc cgtatcagaa aagtttttac
    15421 gcgtttttac ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg
    15481 cgatttttcc ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc
    15541 aatcgcgata tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt
    15601 gttccggctc caggtgccag aggccgaact cacgcacacc aggggaatca atcacatcac
    15661 cgccgtgcgg gaagtgatac aggcgtgcgg cggtcgtggt gtgctgacca agccccgagt
    15721 tgtcagagac atcgttggtc aggatctctt tttgcagccc cagcaacgcg ttcagcaggc
    15781 tggatttgcc gacgccagac tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt
    15841 ccagcggttt tagcccatcc tgagtatggc tggaaaccat caatacgcga taaccgatat
    15901 tgcggtagat atccatctgt tcgttgacga acgccatgcc ttcgtcgtcc agcaggtcta
    15961 tcttgttgag cacaataatc ggctcaatct gcaaggtttc gcaggccacc aggtaacggt
    16021 cgataatatt gagcgacagc tccggcaaaa tggcggagac aatgacaatc tggtcgatgt
    16081 tggcggcaat aggtttcacg ccgtcgtaga aatccggacg cgtcaacact gaggtgcgct
    16141 catgcaccgc ttccacgatc cctttgacat ttacgccttc cgccgccggt ttacccggac
    16201 gccagactac gcggtcgccg gtcaccagcg aacggatggt acggcgaata ttgcagcggt
    16261 gaacgtcgcc atcggcggat tccacatcag cgtgcatacc aaagcggctg atgacgatac
    16321 cttcatcagg ctcgccaaac agattgtcgt cgtagtcggg cttctcctta gacgttttaa
    16381 gacgacgctg gtgattggcg ttcacgcggc gctgctggcc tttggagagt ttatttttac
    16441 tcaatcgtac tgactcctgg tcgcccctga tgggcaaaac atctatgata cacgcaattg
    16501 tggatcaata tagtcactgt gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc
    16561 tgatttggat cgatcttgag atgaccggtc tggatcccga gcgcgatcgc attattgaga
    16621 ttgccacgct ggtgaccgat gccaatctga atattctggc agaagggccg accattgcag
    16681 tacatcagtc cgatgaacag ctggcgctga tggatggctg gaacgtgcgc acccataccg
    16741 ccagcgggct ggtggagcgc gtgaaagcga gcacgatggg cgatcgtgaa gctgaactgg
    16801 caacgctcga atttttaaaa cagtgggtgc ctgcgggtaa atcgccgatt tgcggtaaca
    16861 gcatcggtca ggatcgtcgc ttcctgttta aatacatgcc ggagctggaa gcctacttcc
    16921 actaccgtta tctcgatgtc agcaccctga aagagctggc gcgccgctgg aagccggaaa
    16981 ttcttgatgg ttttaccaaa caggggacgc atcaggcgat ggatgatatc cgtgaatcgg
    17041 tggcggagct ggcttactac cgcgagcatt ttatcaagct gtaaaatttt tgctggtcac
    17101 gtaagcgccg gaatccactt gcggcggcgt gattttgacg ctaaattgaa cgtcttgctg
    17161 ataatttcag cacttgagat aaaaactcaa aaaaaaattt ttaggggggt tgcagaggga
    17221 aagatttctc gtataatgcg cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca
    17281 aaattaagta gtacgcggga atagctcagt tggtagagca cgaccttgcc aaggtcgggg
    17341 tcgcgagttc gagtctcgtt tcccgctcca aaatttgaaa agtgctgcaa agcacagacc
    17401 acccaagcgg gaatagctca gttggtagag cacgaccttg cc
//
LOCUS       DAZKFZ010000081        17347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000081 DAZKFZ010000000
VERSION     DAZKFZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..428
                     /gene="ecpR"
                     /locus_tag="ACHOQW_002513"
                     /gene_synonym="matA"
     CDS             <1..428
                     /gene="ecpR"
                     /locus_tag="ACHOQW_002513"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP9573578.1"
                     /translation="KIRKDFVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSE
                     ALANYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTA
                     QGMLPKSIARIENCSVKTVYTHRRNAEAKLYSKLYKLVQ"
     gene            503..1090
                     /gene="ecpA"
                     /locus_tag="ACHOQW_002514"
                     /gene_synonym="matB"
     CDS             503..1090
                     /gene="ecpA"
                     /locus_tag="ACHOQW_002514"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP9573579.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1148..1816
                     /gene="ecpB"
                     /locus_tag="ACHOQW_002515"
                     /gene_synonym="yagY"
     CDS             1148..1816
                     /gene="ecpB"
                     /locus_tag="ACHOQW_002515"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP9573580.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1842..4367
                     /gene="ecpC"
                     /locus_tag="ACHOQW_002516"
                     /gene_synonym="yagX"
     CDS             1842..4367
                     /gene="ecpC"
                     /locus_tag="ACHOQW_002516"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP9573581.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4357..6000
                     /gene="ecpD"
                     /locus_tag="ACHOQW_002517"
                     /pseudo
     CDS             4357..6000
                     /gene="ecpD"
                     /locus_tag="ACHOQW_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
     gene            5969..6679
                     /gene="ecpE"
                     /locus_tag="ACHOQW_002518"
     CDS             5969..6679
                     /gene="ecpE"
                     /locus_tag="ACHOQW_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP9573582.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6992..7321
                     /gene="ykgJ"
                     /locus_tag="ACHOQW_002519"
     CDS             6992..7321
                     /gene="ykgJ"
                     /locus_tag="ACHOQW_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9573583.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7569..8183)
                     /gene="yagU"
                     /locus_tag="ACHOQW_002520"
     CDS             complement(7569..8183)
                     /gene="yagU"
                     /locus_tag="ACHOQW_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP9573584.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8601..9290
                     /gene="paoA"
                     /locus_tag="ACHOQW_002521"
     CDS             8601..9290
                     /gene="paoA"
                     /locus_tag="ACHOQW_002521"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFP9573585.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9287..10243
                     /gene="paoB"
                     /locus_tag="ACHOQW_002522"
     CDS             9287..10243
                     /gene="paoB"
                     /locus_tag="ACHOQW_002522"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFP9573586.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10240..12438
                     /gene="paoC"
                     /locus_tag="ACHOQW_002523"
     CDS             10240..12438
                     /gene="paoC"
                     /locus_tag="ACHOQW_002523"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP9573587.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPVDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12448..13404
                     /gene="paoD"
                     /locus_tag="ACHOQW_002524"
     CDS             12448..13404
                     /gene="paoD"
                     /locus_tag="ACHOQW_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFP9573588.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(13583..14710)
                     /locus_tag="ACHOQW_002525"
     CDS             complement(13583..14710)
                     /locus_tag="ACHOQW_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9573589.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGRGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSIFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWMVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(14852..15910)
                     /locus_tag="ACHOQW_002526"
     CDS             complement(14852..15910)
                     /locus_tag="ACHOQW_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9573590.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            16156..17058
                     /locus_tag="ACHOQW_002527"
     CDS             16156..17058
                     /locus_tag="ACHOQW_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9573591.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
BASE COUNT         4301 a         4703 c         4600 g         3743 t
ORIGIN      
        1 agaaaatcag aaaagatttc gtgtttatca atctcaatac ggattcttta actgaattta
       61 taaaccgtga taatgagtgg ttatccgcgg taaaggggaa acaggtcgta ttgattgcgg
      121 ccagaaagtc agaagcctta gcaaattatt ggtattacaa cagcaatatt aggggcgtgg
      181 tatacgctgg actgagtcgt gatattagaa aagaactggc ctatgtgatt aatggcaggt
      241 tcctgagaaa agatattaag aaagataaaa tcactgaccg ggaaatggaa attatccgca
      301 tgacggctca gggaatgctg cctaaatcga ttgccagaat tgaaaattgt agtgtaaaga
      361 cagtgtatac ccatcggcgt aatgcagagg ccaagctgta ctcaaaatta tataagttgg
      421 ttcagtaaac tccaggcaag ttagttttaa aaaatgactc actgggacat cacgtcctca
      481 attcaactcg ggaagaaata caatgaaaaa aaaggttctg gcaatagctc tggtaacggt
      541 gtttaccggc atgggtgtgg cgcaggctgc tgacgtaaca gctcaggctg tagcgacctg
      601 gtcggcaaca gccaaaaaag acaccaccag taagctggtt gtgacgccac tcggtagcct
      661 ggcgttccag tatgccgaag gcattaaagg ttttaactca cagaaaggtc tatttgacgt
      721 ggctatcgag ggtgactcaa cggctaccgc ctttaaactg acctcacgtc ttatcaccaa
      781 cacattaacc cagttggata cctcaggttc cacactgaat gtgggcgtgg attataacgg
      841 cgcggcagtc gaaaaaactg gcgataccgt gatgatcgat accgccaacg gcgtactggg
      901 cggcaacctt agcccgctgg caaacggtta caatgccagc aatcgtacca ccgcacagga
      961 tggtttcact ttctccatca tcagcggcac caccaatggt accaccgcag taaccgatta
     1021 cagcactcta ccggaaggca tctggagcgg cgacgttagc gtacagttcg acgcgacctg
     1081 gaccagttaa tctctctgat gtaccagcag gggtagcccc ccagctttat tcctggacgg
     1141 aattgttatg aaaaagcacc ttctgcttct cgctctgctg ttatccggaa tatctccggc
     1201 ccaggcgctg gatgtcggcg atatatcatc gtttatgaac agtgacagca gcacgctgag
     1261 caaaacgatc aaaaacagta ccgacagtgg tcgccttatc aatatccgtc tcgaacggct
     1321 ctcttcaccg cttgacgacg ggcaggttat ctcaatggac aagccggacg agttgctact
     1381 cactcccgcc agcttgctgc tacccgccca agccagcgaa gtgatccgct tcttctataa
     1441 gggaccagca gatgaaaaag agcgctacta ccgcattgtc tggtttgatc aggccctcag
     1501 tgatgcgcag cgcgataatg ccaaccgcag cgctgtggcc actgcttccg cccgcatcgg
     1561 caccattctg gtcgtcgccc cccgccaggc aaactaccac tttcagtacg ccaacggcac
     1621 cctgacaaat acaggaaatg cgacgctgcg gatcctcgcc tacggcccct gcctgaaagc
     1681 cgccaatggt aaagagtgta aagagaatta ctacctgatg ccgggcaagt cgcgtcgttt
     1741 tacccgcgtg gacacggcgg ataacaaagg acgggttgca ctttggcagg gtgataagtt
     1801 cattcccgtg aaatagatag ctgtgcagat ggataacgac aatgccttta cgacggttct
     1861 ccccaggact gaaagcccag tttgccttcg gcatggtctt tttgttcgtt cagcccgatg
     1921 ccagcgctgc tgacataagt gcgcagcaaa taggtggggt gattattccg caggccttca
     1981 gtcaggcgct tcaggacggc atgagcgtcc cgctctatat tcatctcgcc ggtagccagg
     2041 gtcgccagga cgatcagcga atcggcagcg cttttatctg gttggatgat ggacagctac
     2101 gcatccggaa aatacagctg gaagagagtg aagataacgc cagtgtcagc gaacaaactc
     2161 gacagcagct gatggctctg gcgaacgccc cgttcaatga ggcccttacc atccccctga
     2221 ctgacaacgc gcagctggat ctcagcttgc gccaactgct gctgcagctg gtggtcaagc
     2281 gcgaagcgct gggcactgta ctacgctcac gtagcgaaga catcgggcag tccagtgtta
     2341 acaccctcag cagtaatctg agctataact tcggcatcta taacaaccag ttgcgtaacg
     2401 gcgggagcaa cacatccagc tatctgtcgc tgaataacgt tactgcactg cgcgaacatc
     2461 atgtggtgct cgacggctcg ctgtacggga tcggtagcgg tcaacaggac agtgaattat
     2521 ataaagcgat gtatgaacgc gattttgccg gtcaccgatt tgccggtgga acgctcgaca
     2581 cctggaactt gcagtcctta gggccgatga ccgccatttc agcagggaag atttacggcc
     2641 tttcctgggg aaaccaggcc agctccacca tcttcgacag cagccagtca gccacgccag
     2701 tgatcgcctt tttaccggcg gcgggtgaag tacatctcac ccgtgatggg cggttactaa
     2761 gcgttcagaa cttcaccatg ggcaatcatg aagtggatac ccggggtcta ccatacggta
     2821 tttacgatgt ggaagttgag gtgatcgtta acggtcgcgt gatcagcaaa cgcacccagc
     2881 gggtcaataa gctgtttagc cgggggcgcg gcgtcggtgc accactggcg tggcaggtat
     2941 ggggcggtag ctttcatatg gatcgctggt cggaaaacgg gaaaaagacg cgaccagcta
     3001 aagagagttg gctggcaggt gcctcgacct ccggctcact gagtacgctt agctgggcgg
     3061 caacgggata tggatacgat aatcaggcgg tgggtgaaac ccgtctgacg ctgccgcttg
     3121 ggggagcgat caacgttaac ctgcaaaata tgctggccag tgacagctca tggagcagca
     3181 tcggcagcat cagcgccact ctaccgggag gctttagttc gctgtgggtt aatcaggaaa
     3241 aaacccgcat tggcaatcaa ttgcgacgta gcgatgccga caaccgtgct atcggcggca
     3301 cactcaacct gaactcactg tggtcgaagc tgggcacatt cagcatcagc tacaatgatg
     3361 accgccgtta caacagccat tattacacgg cagattacta tcaaaatgtc tacagcggta
     3421 cctttggttc gcttggcctg cgggccggta ttcagcgcta taacaacggc gacagcaacg
     3481 ccaatacagg gaaatatatc gctctcgatc tctcgctacc actgggcaac tggtttagcg
     3541 cagggatgac tcatcaaaac ggctacacca tggcaaacct gtcagcacgc aagcagtttg
     3601 atgaagggac cattcgcact gttggtgcca atctgtcacg agccatctcc ggcgataccg
     3661 gtgatgacaa aactctcagt ggtggggcgt atgcacagtt cgacgctcgc tacgccagcg
     3721 gaacgctgaa cgtcaatagc gcggcggacg gctacgtcaa taccaattta accgccaatg
     3781 gcagcgtcgg ctggcagggt aaaaacattg ctgccagcgg gcggactgat ggcaacgctg
     3841 gggtgatatt caacaccggg ctggaggacg acggtcagat cagcgccaaa atcaacgggc
     3901 ggattttccc gcttaacggc aagcgtaact atctcccgct ctctccctat ggaagatatg
     3961 aggtggagtt acagaacagc aaaaactcac tcgacagtta cgatatcgtc agcggtcgca
     4021 aaagtcatct gactctctat ccaggcaatg tcgctgtcat tgagccagag gtgaagcaga
     4081 tggttaccgt ctccggtcgt atccgtgcgg aagacggcac actgctggct aacgcacgga
     4141 ttaacaacca tatcggccga acccgaaccg atgaaaacgg cgagtttgtc atggacgtgg
     4201 ataagaaata ccccactatc gattttcgct acagtggcaa taaaacctgc gaagtggcac
     4261 tggaactcaa ccaggcgcgc ggtgccgtct gggtcggtga tgtggtctgc agcggcctct
     4321 catcgtgggc ggcggtgacg cagacaggag aagagaatga gagttaacct actgatagcg
     4381 atgataatct ttgcgctaat ctggccagta actgcgctca gagcggcagt gagcaaaaca
     4441 acctgggcgg atgcaccggc acgcgagttt gtgtttgtcg aaaacaactc agacgacaac
     4501 tttttcgtca ctcctggcgg ggcgctggat ccgcgcctga ccggtgccaa ccgctggacc
     4561 ggtttaaaat acaatggttc aggaaccatc tatcagcaaa gcctcggcta cattgataac
     4621 ggttacaaca ccggccttta taccaactgg aagtttgata tgtggctgga aaattcacca
     4681 gtttcatctc ctttaactgg cttgcgctgc atcaactggt acgctgggtg taatatgacc
     4741 accagtctta tcctgccgca aaccaccgac accagtggat tttatggcgc gacggtgacc
     4801 agcggcggcg cgaagtggat gcacggcatg ttgtcagacg cgttttacca gtatctgcaa
     4861 caaatgcccg tcggcagcag ctttacaatg accatcaatg cctgccagac ctctgtgaac
     4921 tatgacgcca gcagcggcgc acgctgtaag gatcaggcct ccggcaactg gtatgttcgc
     4981 aacgtcaccc atacgaaagc agcaaatcta cggttgataa atacccactc gctggcggaa
     5041 gtatttatca acagcgacgg agtaccgact ctgggcgaag ggaacgccga ctgccggacg
     5101 caaaccatcg gcagccgttc aggattaagt tgtaagatgg ttaactatac cctgcaaaca
     5161 aacggactca gcaacacctc aatccatata ttcccggcga tcgccaactc gtcgttagcc
     5221 tcggccgtcg gggcgtacga tatgcagttc agtctgaatg gcagttcatg gaaaccggtg
     5281 agcaatactg cctattacta caccttcaac gagatgaaga gcgcagactc gatctatgtt
     5341 ttcttctcga gcaacttctt taagcagatg gtgaaccagg gaatcagcga tatcaacacc
     5401 aaagatctat tcaactttcg ctttcagaac accacatcac cggagtctgg ctggtatgaa
     5461 ttttctacct ccaacacgct gattatcaaa ccccgtgatt tcagcatcag tattatctcc
     5521 gatgaatata ctcagacacc gtcgcgggag ggatatgtcg gcagcggcga gtcggcactc
     5581 gatttcggct atatcgtaac caccagcggt aaaacagctg ccgacgaagt gctgatcaag
     5641 gtgaccggac ccgcgcaggt gattggcggg cgctcctatt gtgtcttcag ctccgatgac
     5701 ggtaaggcga aagtaccgtt cccggcgacg ctttccttta ttacccgcaa cggagctaca
     5761 aaaacctacg atgccgggtg cgatgatagc tggcgggata tgaccgatgc gctgtggttg
     5821 accacaccgt ggactgatat ctctggcgaa gtggggcaga tggataagac cacagtcaaa
     5881 ttttcgattc caatggataa cgccatttct ctgcgtacgg tagatgataa cggctagttt
     5941 ggcgaagtca gcgcttcagg agaaattcat gttcaggcga cgtggcgtaa cattaactaa
     6001 ggccctgctg acagcggtct gtatgctggc ggcacctttg acacaggcga tttcggtcgg
     6061 caatctgact ttttcactgc cgtccgagac tgactttgtc agcaaacgtg tagtgaataa
     6121 caacaaaagc gcgcggatat accgtattgc catcagtgct atcgatagcc cgggcagcag
     6181 tgaattacgc acccgaccgg tggatggtga actgcttttc gccccccgcc agctggcgtt
     6241 gcaggctggt gagagcgagt attttaaatt ttactatcat gggccacagg ataaccgcga
     6301 gcgctactac cgggtctcat ttcgcgaggt ccccactcgt aacctgacaa aacgcagccc
     6361 taccggcggt gaggttagca cggagccggt ggtggtgatg gataccattc tggtagtacg
     6421 accacgtcag gttcagttta aatggtcctt cgatcaggtg accggaacgg taagtaacac
     6481 cggcaacacg tggtttaagc tactgattaa accaggatgt gattcgaccg aagaggaagg
     6541 cgatgcctgg tatctacgtc cggaagacgt ggttcatcag cctgagttac gtcagccggg
     6601 gaatcactat ctggtctata acgacaaatt cattaagatt agcgattcct gtccggctaa
     6661 gcccccttcg gcggactgat acttatccat cggtcgaggg aagaattcca cctcagagac
     6721 tcaaaaatcg tttaaatgat gtggaagcga tcgtcaatat cacctgtaac gataggcttt
     6781 ctcatatgca caccacagtg ttcaggagac acggaaaggt accaggcggc ctgctaacga
     6841 gtggtttctt cactaattag gacgtaaaat cggtaacggc tggaaatcat tcaatactca
     6901 cactatcgaa agtacaccag ccaaccgcga tacgtccctg catacgacgt accgcagttc
     6961 tctttacgat ttatagctgt actggtgaat tatgagcaat ctgaatccat gcatgacgtg
     7021 tggtgcctgt tgtgcatttt tccgcgtctc tttctactgg gccgaagccg acgatgctgg
     7081 cggcactatt cccgccaggc tcactgaaca aatatcccct tttcaccgat gcatgagcgg
     7141 aactaatcag aaaaaccccc gatgtattgc ccttgcagga accccgggca aaaatgcctg
     7201 ctgcacgata tataaaaatc gatcgtccac atgcagagaa ttcgccatgt ctggtgagaa
     7261 cggagtcgtc aatgaggctt gcaatcgtgc aagggctaaa tacgggctga caccgttata
     7321 aatatacaat aattatttgc actgccccgc cagctgtaat agcggggctt cgtttttcag
     7381 gggtaacaaa ccccgatact tcttctattt gccagcaaca atgcctctct tctgtagcgt
     7441 tcttcggatc tacatcatcc tgaagtagcg cgatatactc actgactatc tgaatcagaa
     7501 tataaaaagc aatgttttta acctataaaa atggcgctgt atttgcgcca tttttatcat
     7561 tcaatgcatt atctgtttga gcctaaaggg atctcagggt ctggctcatg agtaattctg
     7621 tttcgtaaat ctctgcgaat aatttcaatg gaccagaacc agactaaatg tccaaaaatt
     7681 tcagaaacat tctcatacca cgggagatca aacagaggtg gtgtcagtcc catgagaggg
     7741 aatgaaatca tatgaacaaa aagttgggct aaagcacctg ccagtaagcc ctgccagagt
     7801 ttaatttttg gaaatacttc tgcgaccaca caataaccga cagcaaacac tatcgaaaag
     7861 ataatgtgcg taacaccaac ccagttaaag acatgcccgg caaaggtata aacagccgca
     7921 ttgggatctg tcagccccag ccagtctcga agaaaaatat acggaggatt gagaaaatta
     7981 cgcgagcaat caatttgccc agcagccctg attaatgatt ccgggccaca cgctgcatta
     8041 aacatatcca ccgggctacg tggcggcaat ggaacttcag ccccccactt cacaaatgcg
     8101 gaaacaacgc cagcaatcag cccaatgaat gcagcaagac cataacgtct gcggttcggt
     8161 ggagtttgtt caaatatatt catatctacc ctgcttgtac cattatgtta tacacctctt
     8221 taggagtatt cataaaacaa ggtaaatgta aagaactgta ttgttttgta taacaagatg
     8281 gttgtctaat caccaaagaa tataagcacc atcatttctc cctattttta tattagaagt
     8341 gacatagatt tgcagggtga tgcagagctg acaatcacag gtttccttat cggtttttgc
     8401 atcgtacaac taaagcaata aaccagctcc catcatcatc aacatcccgg cgaacatgaa
     8461 tttattagcc gggctcatag ctgcattcgc gttttaagaa taatcctcct gctgtcgccg
     8521 actatgctta acgtttaaaa aagcatcagc actctcgcaa cgcactctta ttttcccctt
     8581 tagaataccg gaggcctggt atgagcaacc aaggcgaata ccccgaagat aatcgggttg
     8641 ggaagcatga gccgcacgat ttaagtttga cccgtcgcga tctgattaaa gtgagcgccg
     8701 caacggcggc ggccgccgtg gtttatcctc attctacgct tgcggcaagc gttccggcag
     8761 ctacacccgc gcctgagata atgcccctga cacttaaggt gaacggcaaa accgagcagc
     8821 ttgaggtgga tacccgaacc acgctactgg acgctttgcg tgaaaatctg catttgatcg
     8881 gtaccaagaa aggttgcgat cacggacagt gcggagcctg taccgtgctg gtcaatggtc
     8941 gcaggcttaa tgcctgcctg acgcttgcag tcatgcatca gggggccgag atcaccacca
     9001 ttgaaggcct gggttcacca gataatcttc accccatgca ggcggccttt atcaagcatg
     9061 atggcttcca gtgcggctac tgcacctccg ggcaaatttg ctcatcagta gcggtgctaa
     9121 aagagattca ggacggcatt cccagtcacg tcacggtcga tttggtttcc cctccagaaa
     9181 gaactgccga tgagatccgt gaacgtatga gcggcaacat ctgtcgctgt ggtgcatacg
     9241 ctaacatcct tgccgccatt gaagacgctg cgggggagat aaaatcatga aggcgtttac
     9301 ctatgaacga gtgaacaccc ccgccgaggc ggcacttagc gctcagcgcg tacccggcgc
     9361 aaaatttatc gcgggcggga ccaatctgct ggacctgatg aagctggaaa ttgaaacgcc
     9421 cacccacctt atcgatgtga acgggctcgg gctcgataag atcgaagtga ccgacgcggg
     9481 tgggctgcgc atcggcgcac tggtacggaa caccgacctg gtggctcacg agcgcgtgcg
     9541 tcgtgattac gcggtactct cccgcgccct gctcgctggc gcgtctggtc agttacgcaa
     9601 tcaggcaacc acagcaggta atctgctcca gcgcacgcgc tgcccctatt tttacgacac
     9661 caatcagccc tgcaataagc gcctgcccgg gagcggctgc gcggcgcttg aaggctttag
     9721 ccgtcagcac gcggtggtag gcgtaagcga agcctgcatt gccacccatc cgagcgatat
     9781 ggcggtcgca atgcggttgc tggatgcggt ggtggaaacc atcacgccgg agggaaagac
     9841 tcgcagtatc acactggctg atttttatca cccaccgggg aaaacgccgc acattgaaac
     9901 cgccctgctt cccggtgagc ttatcgttgc ggtgacgtta cctccgccgc tcggcggaaa
     9961 acatatctac cgtaaggtgc gcgatcgcgc ctcctacgcc tttgccctgg tatcggtcgc
    10021 ggcgattatt cagcctgacg gcagcgggcg cgtcgcgctg ggcggagtag cacataagcc
    10081 ctggcgcatt gaggctgcgg atgctcagct atcccagggg gcgcaggccg tatatgacgc
    10141 gctgttcgcc agcgcccatc ccaccgctga aaacaccttt aaactcctgt tggcgaagcg
    10201 aacgcttgcc tccgtactgg ctgaagcgag ggcacaagca tgaaatttga taaacccgca
    10261 ggggaaaacc cgatcgatca gctgaaggtt gtcggtcgtc cccatgaccg catcgacgga
    10321 ccgctgaaaa ctaccggcac ggcacgctac gcctacgaat ggcatgaaga atcccccaac
    10381 gccgcctatg gctatatcgt cggttccgcc attgccaaag gacgcctcac cgcccttgat
    10441 acggacgccg cgcaaaaagc gccgggcgta ctgcctgtca ttaccgccag taacgccggg
    10501 gcactcagta aaggcgacaa aaacaccgcc aggctgttag gcggccccac tattgagcac
    10561 tatcatcagg ccattgctct ggtagtggcc gagaccttcg aacaggcgcg agcggcggcc
    10621 tcgctggtgc aggcgcacta tcgccgtaat aaaggagctt actccctggc ggacgaaaaa
    10681 caggccgtca gtcagccgcc ggtagacacg cccgacaaga acgtcggtga ctttgacggg
    10741 gctttctcct ccgctgcggt gaagattgat gctacctaca cgaccccgga ccagagccat
    10801 atggcgatgg agccgcatgc ctcgatggcc gtctgggatg gaaataagct tactctctgg
    10861 acctcaaatc agatgattga ctggtgccgc accgatctgg caaaaacgct gaaagtgccc
    10921 gtggagaatg tgcgtattat ctccccgtat atcggcggag ggtttggcgg caagctgttc
    10981 ctgagaagcg atgcgctgct ggcggccctc gccgcccgag cggtgaaacg tccggttaaa
    11041 gtgatgctcc cccgcccctc tattcccaat aacaccacgc accgccccgc cacccttcag
    11101 cacttgcgta tcggtgccga ccagagcggg aaaatcaccg ctatctcaca tgaaagctgg
    11161 tctggaaacc tgcccggcgg cacgccggaa acggcggtac agcaaagcga attactctac
    11221 gccggggcaa accgtcatac cggcctgcgg ctcgccacgc ttgatttgcc ggaagggaac
    11281 gccatgcgtg cgcccggcga agcccccggt ctgatggcgc tcgaaatcgc gatcgacgaa
    11341 ctggcggaaa aagcgggcat cgatcccgtc gagtttcgca tcctgaatga cactcagatt
    11401 gaccccgccg acccgacgcg ccgcttctct cgccgtcagc ttatcgagtg cttgcgcacc
    11461 ggagcggata aatttggttg gaagcagcgc aacgccacac ccggacaggt gcgcgacggg
    11521 gagtggctag tcggccacgg cgtcgcggcg ggctttcgca ataatctgct ggaaaaatcg
    11581 ggggctcggg ttcacctcga accaaacggc accgttaccg tggaaacgga catgaccgac
    11641 attggcaccg gcagctacac cattctggcc cagacggcag cggaaatgct tggcgtaccg
    11701 ctggagcagg ttgcggttca cctcggcgat tccagtttcc cggtttctgc gggttctggt
    11761 ggacaatggg gcgcgaatac ctccacctcc ggcgtttacg ccgcctgtgt gaagcttcgc
    11821 gaaatgattg cctcggcagt cgggtttgat cctgagcagt cgcagtttgc cgacggcaag
    11881 attaccaacg gtacccgaag cgccatacta catgaggcca ccgcaggcgg cagactgaca
    11941 gcggaagaga gcattgaatt cggaacactg agcaaggagt accagcagtc gacctttgcc
    12001 gggcattttg tggaggtcgg cgtgcatagc gcgacgggag aagttcgggt ccggcgtatg
    12061 ctcgctgtgt gtgctgcagg acgcatcctg aatccgaaaa ctgcacgcag ccaagtcatt
    12121 ggcgcaatga ctatgggcat gggcgcggca ctgatggagg agctggcggt ggatgaccgt
    12181 ttgggctact tcgttaatca cgatatggcg gggtatgagg tgccggtcca tgcggatatc
    12241 ccaaaacagg aagtgatttt cctggatgat accgacccca tatcctcccc gatgaaggcc
    12301 aaaggtgtcg gtgagctggg cctgtgcggc gtgagcgcgg ctatcgccaa cgcggtgtat
    12361 aacgccaccg gtattcgggt acgcgattat cccatcactc tggataagct gctcgataag
    12421 ctgccggatg tggtttaagg aggaacaatg tcatacccgc tttttgataa agacgaacac
    12481 tggcataagc cagagcaggc gtttctcacc gatgaccacc ggaccattct gcgcttcgcc
    12541 gtagaggcgc taatgtccgg taaaggagcg gtgctggtga cgctggtgga gatacgcggc
    12601 ggcgcggcgc gcccgctcgg ggcgcagatg gtggtgcgcg aagatggtcg ttactgcggt
    12661 tttgtctctg gcggctgcgt ggaggccgct gccgcttttg aagcgctgga gatgatgggc
    12721 tcaggctgcg atcgcgaaat tcgctatggc gaaggttcgc cgtggtttga catcgttctg
    12781 ccctgcggcg gtgggatcac gctgacgctc cataaactac gctcggcaca gcctctgctc
    12841 gccgtgctga accgcctgga acagagaaaa ccggcggggc tgcgctacga tccgcaagca
    12901 caatcgttgg tgtgcctgcc cacgcaaacc cgaacgggct ggaatctcaa tggctttgag
    12961 gtggggttca ggccatgcgt caggctgatg atttacggac gttctcttga ggcgcaggca
    13021 accgcgagtc ttgcagcagc cacaggctat gacagccata tcttcgatct ttttccggcc
    13081 tcagccagcg ctcagatcga taccgatacg gcggtcattt tgctgtgcca tgatctcaac
    13141 cgggagctgc cagtgttgca ggccgcgcga gaagcaaaac ccttttatct cggcgcattg
    13201 ggcagctatc gaacccacac gttacgtctg caaaagctcc acgagctggg atggtccagg
    13261 gaggagacag cgcaaatccg ggcacccgtc gggatattcc ccaaagcccg gaatgcgcat
    13321 acactggccc tctccgtgct ggcagaagtc gcctctgtac gtctccatca ggaggaggat
    13381 tcatgcctgc ccccgtcgtc ctgatccttg cggccgggcg tggaaagcgc tttctcacct
    13441 ccgggggaaa taccataaat taagagaaaa atttgcagta gccgttttag aaaatcacct
    13501 aaatcgttta aaagagcgcc ctacccatag tggttgctca tcgccttgtg taactttttc
    13561 agcaacgcgc gccctcgccg gactaaagcg caccggcctt ctggcgtgct gtaacaaagg
    13621 tcagtacgcc cgcgcaaagg agcagcacac tactcatggc aaacgtactc tgccagccaa
    13681 ggcggtcaaa cacgatgcct cctgccgttg atccaagggc gattgagagc tgaatcaccg
    13741 cgaccatgag cccgcctccg gcttcggcgt tgtcaggcaa cgtacgcgca atccatgtcc
    13801 accatccggt tggcgcggcg gtggccagca tgccccagag tcctaacagc agcgagacca
    13861 tccagacgtg atgcccggta agcatcagac cgatggcaat gacggccatc agtccgggta
    13921 tggccatcag cgtggggtag aattttctgt tgagaaatat cgaaacaacc agcgtgccaa
    13981 taaaaccggc cacgccgata atcagcaaaa tcaacgacaa gcctaaggaa ttaacccgtg
    14041 tcaccgactc cagaaatggg cgcacatagg tgaatagcgc aaactgcccc atgaaaaaca
    14101 ggccacaagc tatcatgccg accacaacca tgcggcggct aaataagcgg aataccgcgc
    14161 cacatgacgt gtggctttta ttcgcatcca tgctgggcag actaatgcac tgccagataa
    14221 aggcgacaac cgccatgggc accaagcaca agaaagcgcc tcgccatccg accgtagccc
    14281 ccagatagct tcccagcggc gcggccacga ccgtcgccag cgcattaccg gcgttgaaaa
    14341 tcgccagcgc acgcgtgacc tgatgctgcg gcaccagacg aatcgccgtt gccgctgaca
    14401 tcgaccagaa tccgccaatg gccacaccaa tcatcgcacg accgaccata tacatcagat
    14461 aactggtagc cagcgcaata ataagccccg atacggccat cagaaccgtc atccccagca
    14521 gcaggaactt acgattcatc tttccagcta gcgttgaaag cgtcaggctg gtcagaaccg
    14581 ccagcgcacc ggagatagca atcccccgcc ctgccagccc ctccgtcacg cccaggtcac
    14641 gggcaatcgg cgtgagcagg ctgacgggca taaattcaga agcaatcagc acaaacacgc
    14701 acagggtcat ggcaaaaata ccgccccagt atgcgtgctg atgatgaggt gatggtgttt
    14761 ggcttagtgt cgacatagtg aaatttcaca aagatcagga taaaagccac cggccataga
    14821 ccggtggctt tgcatcaatt caggggaggt tttatttcag tttggtctgg aaaaattgtt
    14881 cgaacttcgc gaaaggtatt tttccggcca cgttatcgta caggtccacg tgatttgccc
    14941 ctggtaccac caccaactct ttgtctttac tgccaaccgc cttgtaagca tcttcagcaa
    15001 aatagcgtga atgtgctttc tcaccggtca caatcagcgt cggaatggtg atttcactgg
    15061 cgtagctcaa cagcggcata ttcataaatg acagcggcat cgttgccgtc cacgccccgg
    15121 tagagttaac ggaacgttcg tggaagccgc gcggcatgcg atagtagtca aagaactctt
    15181 tcagtaccgg atgaggattt gccggcagcg tttctggcag aatgcgctcg cccgcactga
    15241 ccttgccgtt gctatcgaca ttaatatcat gaccaccgtg agcgaacgtt ccgctctgcg
    15301 catccttcca gcgctgttca ttcaaatatt gcagaacagc acgacggtcg gcggtagaat
    15361 aacggtcttc gccatccccc acaccatggc ccatcgcccg gctcatgtca tacatcacgc
    15421 tggtagcaac cgctttaacg cgggtatcca tcgccgcgtc gttcagcgcc atgccgcccc
    15481 agccacaaat accgagcagc ccgatacggt tgcgatctac ctctttttgc agccctaaga
    15541 aatccaccgc cgcgctgaaa tcttcagtgt tgatatccgg tgaggcgaca tttcgcggat
    15601 agccgccgct ttccccggtg taagaaggat cgaacgccag ggtaacaaac cctcgttccg
    15661 ccagcgtctg cgcatacaag ccgctggatt gctctttcac cgcgccaaat ggcccactga
    15721 ccgcaatcgc cgccagcttg cgatcgccac ggtctttagg caggtaaaga tcgcccacta
    15781 aggtgatgcc atatcggttc gggaacgaga ctttacggtg atcgactttc tggctctcgg
    15841 cgaacgtttt atcccattta tcggtcatgg acacaggggc attaggattg gttgaatcag
    15901 cataactcat tgtcgtgact ccacttaatg atgcgaaaag cagcatgacc ggcatcgcgg
    15961 tttttagcgt tcgggtgaag gctttcataa aaatccccat aaaaaacaac agagagatac
    16021 aggtttccga cggacaacgc tgcggtgctt tactttgatg gggtcattat agtgagcaga
    16081 attagtgctg attagggggc gattaatgct aagattaata ccatattgtt ataaattaat
    16141 gctgagattg ccggcatgaa ggggaatgtt tcaatggcaa aaagggagaa ctacaacgag
    16201 ctatacctgt ttatgcaggt ggtgcgggaa ggcagcttta ccgcagcggc taagcggctg
    16261 ggtcttgctc agtcaggagt cagccgttct gttcgcgagc ttgaagaaag gctgggcgtc
    16321 cagctgctgg tgcgcaccac gcgtaaactg tcgctgacgc aagcaggcga gcagctctac
    16381 cagaagacgg catctggatt tgaaatgtta gatcttgggc ttgccacgct ggcgcactat
    16441 cgagagacgc cctccggcac ggtgcgtatc aatgccagcc agcacgcgat tgataaatgc
    16501 ctgctgccaa agctggcggt atttaaacag cgatatcctg atatccagct tgaactgata
    16561 aatgagagcc ggttcgtcga tatcatcgag gaaagattcg atgcaggtgt ccgcctgggg
    16621 cctgaagtca gccagggcat ggtcgcggta cgcatcacgc ccgatatgga gatggcaatt
    16681 gtggggactc cggaacattt tcgtcgctat ggttttccgc aaaccccggc ggatttaaag
    16741 gcgcatccct gtatcgccta tcagtttgcc gacggcagcg tataccagtg ggaactcaat
    16801 caggatgata aaaaaatcac tcatcaaccc gaaggacaat gggccttttc cgacagctat
    16861 atggaagcag aagccgcccg gctgggcctc gggttggcct atgttcctgt tgaactggtc
    16921 gcggatgatt tagaacacgg taagcttatc agagtgttgc agcgttacag cctgcgaatg
    16981 gagggattgt ttctctatta ccctcatcgc aacgtctccc ccgctctgcg gatggtcatt
    17041 gatacattga aaatctgaac gttaacaacg accgactctt cactgtgcaa tattgtgtca
    17101 ggtagtcact ctgcgttcaa ttccaggggc cgctttgagc gagaaagagc ggacttatct
    17161 gtacaagggg actatccagc aatggagatc atccaaaaga aaaaaccacc tggctcccct
    17221 cgacaacctt aatccgggca tttagcctca atcaacattt tcatatctgt caggttactt
    17281 cattatgaaa accgatcaat accgttgttt ttgttgcaac agtagaatac gcgtatttat
    17341 ccctcca
//
LOCUS       DAZKFZ010000082        17345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000082 DAZKFZ010000000
VERSION     DAZKFZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1386)
                     /gene="djlB"
                     /locus_tag="ACHOQW_002528"
     CDS             complement(<1..1386)
                     /gene="djlB"
                     /locus_tag="ACHOQW_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP9573592.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFI"
     gene            complement(1383..2090)
                     /gene="ybeR"
                     /locus_tag="ACHOQW_002529"
     CDS             complement(1383..2090)
                     /gene="ybeR"
                     /locus_tag="ACHOQW_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9573593.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2255..3232
                     /gene="ybeQ"
                     /locus_tag="ACHOQW_002530"
                     /pseudo
     CDS             2255..3232
                     /gene="ybeQ"
                     /locus_tag="ACHOQW_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(3302..3784)
                     /gene="ybeL"
                     /locus_tag="ACHOQW_002531"
     CDS             complement(3302..3784)
                     /gene="ybeL"
                     /locus_tag="ACHOQW_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP9573594.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4020..6602
                     /gene="leuS"
                     /locus_tag="ACHOQW_002532"
     CDS             4020..6602
                     /gene="leuS"
                     /locus_tag="ACHOQW_002532"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9573595.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6617..7198
                     /gene="lptE"
                     /locus_tag="ACHOQW_002533"
     CDS             6617..7198
                     /gene="lptE"
                     /locus_tag="ACHOQW_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP9573596.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7198..8229
                     /gene="holA"
                     /locus_tag="ACHOQW_002534"
     CDS             7198..8229
                     /gene="holA"
                     /locus_tag="ACHOQW_002534"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9573597.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8231..8872
                     /gene="nadD"
                     /locus_tag="ACHOQW_002535"
     CDS             8231..8872
                     /gene="nadD"
                     /locus_tag="ACHOQW_002535"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9573598.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            8896..9507
                     /gene="cobC"
                     /locus_tag="ACHOQW_002536"
     CDS             8896..9507
                     /gene="cobC"
                     /locus_tag="ACHOQW_002536"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP9573599.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9767..10084
                     /gene="rsfS"
                     /locus_tag="ACHOQW_002537"
     CDS             9767..10084
                     /gene="rsfS"
                     /locus_tag="ACHOQW_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9573600.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10088..10555
                     /gene="rlmH"
                     /locus_tag="ACHOQW_002538"
     CDS             10088..10555
                     /gene="rlmH"
                     /locus_tag="ACHOQW_002538"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9573601.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10586..12487
                     /gene="mrdA"
                     /locus_tag="ACHOQW_002539"
     CDS             10586..12487
                     /gene="mrdA"
                     /locus_tag="ACHOQW_002539"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP9573602.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12490..13602
                     /gene="mrdB"
                     /locus_tag="ACHOQW_002540"
                     /gene_synonym="rodA"
     CDS             12490..13602
                     /gene="mrdB"
                     /locus_tag="ACHOQW_002540"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP9573603.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13613..14701
                     /gene="rlpA"
                     /locus_tag="ACHOQW_002541"
     CDS             13613..14701
                     /gene="rlpA"
                     /locus_tag="ACHOQW_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP9573604.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14841..16052
                     /gene="dacA"
                     /locus_tag="ACHOQW_002542"
     CDS             14841..16052
                     /gene="dacA"
                     /locus_tag="ACHOQW_002542"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP9573605.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16162..16425
                     /gene="ybeD"
                     /locus_tag="ACHOQW_002543"
     CDS             16162..16425
                     /gene="ybeD"
                     /locus_tag="ACHOQW_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP9573606.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16526..17167
                     /gene="lipB"
                     /locus_tag="ACHOQW_002544"
     CDS             16526..17167
                     /gene="lipB"
                     /locus_tag="ACHOQW_002544"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP9573607.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4353 a         4357 c         4432 g         4203 t
ORIGIN      
        1 gataaaaata tagaaaataa ttttaatgat gcccagacag cctggcccac catcgtcaac
       61 gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa aattcgccgc
      121 ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatttt ctgcatcaag
      181 acggcttacg atcggcatta atgattcttc tgtaggttgg ctggcctgca ctaattgttt
      241 ggcaactaga tataatggcc ataaggtatg ttctgtatca cgcgtgagtg agatccgcgg
      301 cttacatcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagagcag agagtttttc
      361 atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg ggacataaag
      421 tagcggtttt ccctggtcgt taaccagttc acgagcttca atcatcatca ccactaatgg
      481 gtaataatcg actcgatgct gacggcacca ttgcaacatt aaagcatccg cctgagtgga
      541 gggatactcg tgccagtagt cacaaagttc tgccagcagg ctttcgcctt ctccacaata
      601 gacgcgttgc gcgtattcat aataacgcgc atcttcatta tcaggttcag cttcccgcca
      661 cgaccgggcg taatccagga gttcggggat tcccaggcga cctgcactat accagcggag
      721 taaatttctg tgcagttttg catcatcggg gataatcact gcgcgatgtt gcgctaaata
      781 caatgtcagc cattctggat gcgatgacca aatccgggcg aacatttggt aaaaatcgat
      841 ggtgtcattt tgcacggcaa cgggcagatg cagaatgatc tggaagttaa aaacatttcc
      901 cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc cgacgttttc
      961 ctcctgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg ataaatgggc
     1021 agtgttcatg gctattgtgc acagcgacca gcgtaattca tcaatctcat tcatcgagca
     1081 ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt cactatcaga
     1141 ggcaagtaac gcacgaaagg cgagcagaat ttcatgttct gctacctcat attcttctgg
     1201 ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcttcctcat acgcctgacg
     1261 aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca gcgccagata
     1321 agcgcggcgg ataatatcga catcagtcgt ttcctctatg ttgaggatct tccagcaatt
     1381 tttcataggt cgctcatatc cttaagcgtt tccgggcatg tggggtagtc agccagtggt
     1441 tgccagggta aggtcgcgta atatgcatcg tcactggcaa ataattgata aaacatatca
     1501 ttgccggaat cgtcgtattc tttacgcgct aagaattcac gtaataaagg cagattatca
     1561 tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta ttgccgccag
     1621 ttactataat aacgtagagc ccgtagatga atacgcgact ggatccatag actttcttct
     1681 tcactcaacc agttattaag tacgcccatt cgacttaaaa aacccatccg gacataatcc
     1741 catgctttaa ttcctccctc accacagcat tcggctgtgc tggcgacaaa ttgcgcataa
     1801 gcctgacctt gctcatttaa ttcagcaaga tagtcacgcc attcacatgg agaataacga
     1861 aaccagcggt gataaaaccc tgccaaccgc gcggcatgac cgttatcgat cattcggtga
     1921 attgtgcgca gcaggtcatc acggtcatca attccccagg aggaagaaag gtcaaaaaag
     1981 cgtgtaccta aatacatttt gggtgatgaa tgcgaaggta aacagcattc attgcgtatt
     2041 tccatcggag tggaaagtgc aaacaatatt ttttgcgact ccatgtccat atgatttatc
     2101 cttaaatatt cattgaatga atattagccg cattgcgacg ctcgtttatt tatacttttc
     2161 tgacgcgaca taatacgcat aaaataaggc ttgcgcaaaa aatgaaacac ataaaaattc
     2221 aatatatttt atctacaatc ataaggaaat gataatgatt ttcacgtcaa gttgctgcga
     2281 taatttatca atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata
     2341 tattgttggg ttttattata atcgcgatag cgcaattgac tctccggacg acgaaaaggc
     2401 cttttactag ctgaagctgg ccgctgagca aggtcattgt gaagcacagt attccttagg
     2461 gcaaaagtat accgaggata aaagccgtca taaagataat gagcaagcca tcttctggct
     2521 gaaaaaagct gccctacaag gccatacttt cgcttccaac gcccttggct ggatactgga
     2581 tcgtggagaa gaccccaact ataaagaagc ggttgcctgg tatcagatag ccgcggagag
     2641 cggaatgtct tatgcgcaaa ataatcttgg gtggatgtac agaaatggca acggagccgc
     2701 acaagactat acgctggcat ttttctggta caaacaagct gcattacaag gccatagtga
     2761 cgcgcaaaac aatctggccg atctttatga agacggaaaa ggcgttgctc aaaacgagac
     2821 actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcatg ctcagtttca
     2881 aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc aagcgatgta
     2941 ctggtatctg aaggctgccg ctcaggggag cgtcggcgct tacgtcaaca tcggttatat
     3001 gtataaacac ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa
     3061 agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt atcactacgg
     3121 agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc
     3181 cggaaccaga gatgtcagtc aggaaattcg tgagactgaa gatttactgt agagataaaa
     3241 acaagggccg gggaatttca ttcggcccat attctcttta ttgctgtgcg aaatcagact
     3301 attacggctc aaacgggcgt ctctggaact ggtcatgacc acatttcggg catagcgtca
     3361 gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc agatttccca
     3421 gcccgaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg aaaacttcgc
     3481 gccattcaag ctgcgtttta tcggtgatgt ctgccagctc ctgccacaag ctttctttaa
     3541 tcacccgcat aaagacgctg tcagattctt ctttcaggct ctcttcatag ctcatggcga
     3601 actcttccag gtcacgtctg acagctcgcg tcagctcatc gacctcggtt cgcgttaact
     3661 cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc
     3721 cattgcgcag gcgttcgctc agtgacgcaa ccagttcacg gtaatattga gcaaccttgt
     3781 tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct ttgttccttg
     3841 ctttgctaat acgcccacaa actctgtaaa ttaatgtatg aataatccgg gcatcatttc
     3901 aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt gggctatgct atgcggatct
     3961 gaaaaaccac ctctaacgct acttttgtag ccgtaatgaa aacaggacca ctggctgcca
     4021 tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat tgggatgaga
     4081 agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc ctgtctatgc
     4141 ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc atcggtgacg
     4201 tgatcgcccg ttaccagcgc atgctgggca aaaacgttct gcagccgatc ggctgggacg
     4261 cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg gcaccgtgga
     4321 cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat gctgggcttt ggttatgact
     4381 ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag aaattcttca
     4441 ccgagctgta taaaaaaggc ctggtatata agaagacttc tgcggtcaac tggtgcccga
     4501 acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata
     4561 ccaaagttga acgtaaagag atcccgcagt ggtttatcaa aatcactgct tacgctgacg
     4621 agctgctcaa cgatttggat aaactggatc actggccaga caccgttaaa accatgcagc
     4681 gtaactggat cggtcgttcc gaaggcgtgg aaatcacctt caacgttaac gactatgaca
     4741 acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc tacctggcgg
     4801 tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa ctggcggcct
     4861 ttattgacga atgccgtaac accaaagttg ctgaagctga aatggcgacg atggagaaaa
     4921 aaggcgtcaa tactggcttt aaagctgttc acccattaac gggcgaagaa attcccgttt
     4981 gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agtaatggcg gttccggggc
     5041 acgaccaacg cgactacgag tttgcctcta aatacggcct gaacatcaaa ccggttatcc
     5101 tggcggctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa aaaggcgtgc
     5161 tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac gccatcgccg
     5221 ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg cgtgactggg
     5281 gtgtttctcg tcagcgttac tggggcgcgc cgattccgat ggtgacgctg gaagacggta
     5341 ccgtaatgcc gaccccggac gaccagctgc cggtgatcct gccggaagat gtggtgatgg
     5401 acggcattac cagcccgatt aaagcagatc cacagtgggc gaaaactacc gttaacggta
     5461 tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc tggtactatg
     5521 cgcgctacac ttgcccagag tacaaagaag gtatgctgga ttccgaagcg gctaactact
     5581 ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac ctgctctact
     5641 tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac gaaccagcga
     5701 aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt ggcgaaaacg
     5761 gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag aaaggccgta
     5821 tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg agcaaaatgt
     5881 ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac ggcgcggaca
     5941 ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa tggcaggaat
     6001 ccggcgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtg tacgagcata
     6061 ctgcgaaagg tgatgttgcg gcactgaacg ttgatgcact gactgaagat cagaaagcgc
     6121 tgcgtcgcga tgtgcataaa actatcgcta aagtgaccga tgatatcggc cgtcgtcaga
     6181 ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg aaagcaccga
     6241 ccgatggcga gcaggatcgc gctctgatgc aggaagcact gctggccgtt gtccgtatgc
     6301 ttaacccgtt caccccgcac atctgcttca cgctgtggca ggaactgaaa ggcgaaggcg
     6361 atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa gactccacgc
     6421 tggtcgtggt gcaggttaac ggtaaagtcc gtgctaaaat caccgttccg gtggacgcaa
     6481 cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg
     6541 gcgttactgt acgtaaagtg atttacgtac caggtaaact cctcaatctg gtcgttggct
     6601 aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg cggtgttaat
     6661 caccgccggg tgtggctggc atctgcgtga caccacgcag gttccttcca ctatgaaggt
     6721 catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcggtgc gtaaccagtt
     6781 acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg ttccatccct
     6841 gcgtttgggt gctgtatcaa tctcacagga taccgcgtcg gttttccgta atggtcaaac
     6901 tgcggaatat cagatggtga tgactgttag cgcgtcggta ttgattcctg gacgagatat
     6961 ctacccgatt agcaccaaag tcttccgttc attcttcgat aacccgcaaa tggcgttagc
     7021 gaaagataac gagcaggaaa tgatcattaa agagatgtac gaccgtgctg ccgaacagct
     7081 gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag aacagacgtc
     7141 gactacaacg gatactccgg caacgcctgc acgcgtctcc accacgctgg gtaactgatg
     7201 attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg cgcggcgtat
     7261 cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctgt tcgtcaggta
     7321 gctgcggcac aaggattcga agaacaccac actttttcca ttgatcccaa cactgactgg
     7381 aatgcgatct tttcgttatg ccaggccatg agtctgtttg ccagtcgaca aacgctattg
     7441 ctgttgttac cagaaaacgg accgaacgcg gcgatcaatg aacaacttct cacactcacc
     7501 ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag caaagcgcaa
     7561 gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac ctgtcagaca
     7621 ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg caaaacagct caacttagaa
     7681 ctggatgacg cggcgaatca ggtgctctgc tactgttatg aaggtaacct gctggcgctg
     7741 gctcaggcac tggagcgttt atcgctgctc tggccagacg gcaaactgac attaccgcgc
     7801 gttgaacagg cggtgaatga tgccgcgcat ttcactcctt ttcattgggt tgatgctttg
     7861 ttgatgggaa aaagtaagcg cgcattgcat attcttcagc aactgcgtct ggaaggcagc
     7921 gaaccggtta ttttgttgcg cacattacaa cgtgaactct tgttactggt taacctgaaa
     7981 cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt atggcagaac
     8041 cgccggggca tgatgggcga ggcgttaaat cgcttaagtc agccgcagtt acgtcaggcc
     8101 gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacagtca gtcagtgtgg
     8161 gcagagttgg aagggttatc tcttctgttg tgccataaac ccctggcgga cgtatttatc
     8221 gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc cggtgcacta
     8281 tggtcatcta aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc gagtcacaat
     8341 catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg tacagcgtaa
     8401 acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg aacgcgagct
     8461 aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc aggaacaagg
     8521 gccggacgtg ccgctggcgt ttattattgg tcaggattca ttgctgacct ttccgacctg
     8581 gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc gtccaggtta
     8641 cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt tgacacataa
     8701 cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa cgccgtggtt
     8761 taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcat gtgatgattt
     8821 attgccggaa ccggtactga cttacattaa ccaacaaggc ttgtatcgct gatacctgtc
     8881 gttacggagg gcatgatgcg actgtggtta attcgtcatg gcgaaacaca agcgaatgtc
     8941 gatggtctgt acagcggtca tgcgcccaca cccctgaccg cacgcggtat tgagcaagcg
     9001 caaaatctgc atacactgct agatgatgtt tcctttgacc tggttttatg cagtgaactg
     9061 gaacgggcac agcataccgc gcgactggtt ctcagtgacc gccagctccc cgttcatatc
     9121 atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca tcgcgacctc
     9181 atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca tgctatcccc
     9241 acgaacggtg aaggatttca ggcattttcg caacgtgtgg aacgctttat cgcaaggctt
     9301 agtgaatatc agcactatca gaatatttta atcgtcagcc atcagggcgt acttagtctg
     9361 ttaattgctc gtttaattgg catgcctgcc gagtctatgt ggcattttcg cgttgaccag
     9421 gggtgttgga gcgccattga tataaaccaa aaattcgcga cgctacgcgt cctcaatagc
     9481 cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa gccgcaacga
     9541 ccattgacag ccccgggcag gctgatattc tccgcagcca gactttttcc gccagacacg
     9601 actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg gtgggatgat
     9661 agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct ggttcaggta
     9721 tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc agggtaaagc
     9781 actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt
     9841 agacgttcag ggcaaatcca gcatcactga ctgcatgatc atctgtacgg gtacgtccag
     9901 ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag cgggcctgtt
     9961 accgctcgga gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt
    10021 gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa aactctggag
    10081 ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact gggtacaaac
    10141 cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc tgattgaaat
    10201 tccggccgga aaacgcggca agaatgcgga catcaagcgc atactcgaca aagagggtga
    10261 gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc caggcaagcc
    10321 ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg gtcgcgacgt
    10381 cagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg cggcggagca
    10441 gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc tggtcgcaga
    10501 gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg agtgataagg
    10561 gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg actatacggc
    10621 agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt tgctgctgac
    10681 cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg actaccagac
    10741 ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg gcattatcta
    10801 cgaccgtaac ggtatccctc tggccctcaa ccgcactatc taccagatag aaatgatgcc
    10861 ggagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg ttgatctgac
    10921 cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt tcacctccat
    10981 tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca atcagtaccg
    11041 ttttccgggt gttgaagtta aaggctataa acgtcgttac tatccttacg gttcggcgtt
    11101 gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa
    11161 tgacggcaaa ctggccaact atgcggcaac gcatgatatc ggtaagctgg gcattgagcg
    11221 ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag ttaacaaccg
    11281 tgggcgtgtt atccgccagt taaaagaagt cccaccgcaa gccggacacg atatttacct
    11341 gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta gccgcgcagc
    11401 tgtggtagtc accgatccgc gtacaggtgg ggtgctggcg ctggtttcca cgcctagtta
    11461 tgacccaaac ttgtttgttg acggtatctc cagcaaagat tattccgcct tgttgaacga
    11521 cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg cgtctacagt
    11581 taaaccctat gtggcggttt cggcattgag cgccggggtg atcacgcgca atacgacgct
    11641 gtttgaccct ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg actggaaaaa
    11701 atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg ataccttctt
    11761 ctatcaggtg gcctacgata tggggatcga tcgcctctcc gaatggatgg gtaaattcgg
    11821 ttatggtcat tataccggta tcgacctggc ggaagaacgt tccggcaata tgcctacccg
    11881 cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg
    11941 tatcggtcag ggttactgga cagcgacccc aatccagatg agtaaggcac tgatgatcct
    12001 gattaatgac ggtatcgtga aggttccgca tttgctgatg agcaccgccg aagacggcaa
    12061 acaggtgcca tgggtacaac cgcatgaacc gcccgttggc gatattcatt ccggttactg
    12121 ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaacggta cggcgcataa
    12181 atactttgcc agcgcaccgt acaaaattgc ggcgaaatct ggtaccgctc aggtcttcgg
    12241 tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac gtgaccacaa
    12301 actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca tgattctgga
    12361 gaacggtggc gcgggtccgg cggttggtac gctgatgcgc cagatcctcg accacattat
    12421 gctgggtgat aacaacaccg atctgcctgc ggaaaatcct gcggttgccg cagcggagga
    12481 ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt ccatctcgat
    12541 cccacaatgc tgctgatttt actggcattg ctggtttaca gcgccctggt tatctggagc
    12601 gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc gatgggtctg
    12661 gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg ggccccctat
    12721 ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggtgc catctctaaa
    12781 ggcgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga aattgccaaa
    12841 atagccgtac cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc gccatcgttg
    12901 aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt ggctgcacag
    12961 cctgacctgg gaacatcaat cctcgttgcg ctttccggtc tgtttgtact gttcctctct
    13021 ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt cattccgatt
    13081 ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct cctggacccg
    13141 gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc tattggctcc
    13201 ggcggattac gcggcaaagg ctggctgcac ggcactcagt cacagcttga atttctcccc
    13261 gaacgccata ctgactttat cttcgcggta ctggcggaag agctgggatt agtgggcatt
    13321 ttgattctgc tcgctctcta cattctgctg atcatgcgcg ggctgtggat agccgccaga
    13381 gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa tgctgatatt attcgtttat
    13441 gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt tccgctccca
    13501 ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg
    13561 tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa
    13621 gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg
    13681 tcagcaacag acagtaagtg taccgcagcc tgcggtatgt aacggcccca tagttgaaat
    13741 tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg attaccagcg
    13801 cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttatccagg cgggactggc
    13861 ggcaatctat gatgccgaac caggcagtaa cctgacggcc tctggcgaag ctttcgatcc
    13921 gacacagctg acggcggccc atccaacgct tccgatcccc agctacgcca gaatcactaa
    13981 cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggtcctt acggcaacga
    14041 ccgcgttatt tcgctttctc gcgcagcagc tgaccgtctt aacacgtcaa acaacaccaa
    14101 agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc ctggtatggc
    14161 ttgtaccaca gtcgccaaac agacttacgc cctgcctgca cctcccgatt taagcggtgg
    14221 cgcgggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca gtaattcgac
    14281 gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagc agcggtttcc tcggcgcacc
    14341 aacgacctta gcgccaggtg tactggaagg cagcgaaccg acgcctgctc cacagcccgt
    14401 tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggtgacac cgcaagccgc
    14461 ctcgcaaagc gccagcggca actttatggt gcaggtcggg gccgtaagcg atcaggctcg
    14521 tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc gcgtaactca
    14581 aaatggcgcg gtctggcgga tccagcttgg cccattcgcc aacaaagccg aagccagtac
    14641 cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacca ccgcgcagta
    14701 gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga
    14761 aagtcagatg cctgccggta gtgcatttgc tatagtaggg cacttttttt aattccatca
    14821 cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg
    14881 cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata
    14941 tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact
    15001 ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc
    15061 tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag
    15121 aaactgattt agtcactatt ggcaacgacg catgggccac cggtaacccg gtgtttaaag
    15181 gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg
    15241 gtataaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta
    15301 gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgccctgggc ctgaaaaata
    15361 cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgcgata
    15421 tggcgctgat cggccaggcg ttgatccgtg acgtaccgaa tgaatactcg atctataaag
    15481 aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata
    15541 acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg
    15601 ttgcttctgc gactgaaggc cagatgcgct tgatttctgc ggtgatgggc ggacgtactt
    15661 ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa
    15721 ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt
    15781 ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct gaccattccg cgtggtcgca
    15841 tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa
    15901 agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc gaacaacgcc
    15961 cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca
    16021 ttaaattaat gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc
    16081 gtccccatat actaagcatc agtaaaaaac tcccgccttc tggcgggagt tgctatttaa
    16141 ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact
    16201 ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa
    16261 gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc
    16321 aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg
    16381 tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta
    16441 cccggcccct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc tcccactttt
    16501 actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc gccagctcgg
    16561 tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga
    16621 tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc aagggcaggc
    16681 aggaaaggcg gagcacattt taatgcccgg tgatattccg gtgattcaga gcgatcgcgg
    16741 tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtattgc ttaacctgaa
    16801 acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac
    16861 cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgccaggtg tctatgttgg
    16921 ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat tccacggtct
    16981 ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc
    17041 cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc
    17101 accacgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg aatatattac
    17161 cgcttaattt catacctcaa tggcccaatt tacactgggt cattactcat aacacctgct
    17221 aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga aatgcgatgc
    17281 tacgccttgc tccgtaactc accccattga ttaacttatt gaaatagttg atcatgatga
    17341 gcaaa
//
LOCUS       DAZKFZ010000083        17253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000083 DAZKFZ010000000
VERSION     DAZKFZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..575)
                     /gene="espY1"
                     /locus_tag="ACHOQW_002545"
     CDS             complement(<1..575)
                     /gene="espY1"
                     /locus_tag="ACHOQW_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFP9573608.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANL"
     gene            complement(870..1685)
                     /gene="djlA"
                     /locus_tag="ACHOQW_002546"
     CDS             complement(870..1685)
                     /gene="djlA"
                     /locus_tag="ACHOQW_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP9573609.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1940..4294
                     /gene="lptD"
                     /locus_tag="ACHOQW_002547"
     CDS             1940..4294
                     /gene="lptD"
                     /locus_tag="ACHOQW_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP9573610.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            4347..5633
                     /gene="surA"
                     /locus_tag="ACHOQW_002548"
     CDS             4347..5633
                     /gene="surA"
                     /locus_tag="ACHOQW_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP9573611.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5633..6622
                     /gene="pdxA"
                     /locus_tag="ACHOQW_002549"
     CDS             5633..6622
                     /gene="pdxA"
                     /locus_tag="ACHOQW_002549"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP9573612.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6619..7440
                     /gene="rsmA"
                     /locus_tag="ACHOQW_002550"
     CDS             6619..7440
                     /gene="rsmA"
                     /locus_tag="ACHOQW_002550"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9573613.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            7443..7820
                     /gene="apaG"
                     /locus_tag="ACHOQW_002551"
     CDS             7443..7820
                     /gene="apaG"
                     /locus_tag="ACHOQW_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP9573614.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7827..8675
                     /gene="apaH"
                     /locus_tag="ACHOQW_002552"
     CDS             7827..8675
                     /gene="apaH"
                     /locus_tag="ACHOQW_002552"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP9573615.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(8672..8986)
                     /locus_tag="ACHOQW_002553"
     CDS             complement(8672..8986)
                     /locus_tag="ACHOQW_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFP9573616.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(8989..9222)
                     /locus_tag="ACHOQW_002554"
     CDS             complement(8989..9222)
                     /locus_tag="ACHOQW_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP9573617.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(9308..9787)
                     /gene="folA"
                     /locus_tag="ACHOQW_002555"
     CDS             complement(9308..9787)
                     /gene="folA"
                     /locus_tag="ACHOQW_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP9573618.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(9979..11841)
                     /gene="kefC"
                     /locus_tag="ACHOQW_002556"
     CDS             complement(9979..11841)
                     /gene="kefC"
                     /locus_tag="ACHOQW_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP9573619.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11834..12364)
                     /gene="kefF"
                     /locus_tag="ACHOQW_002557"
     CDS             complement(11834..12364)
                     /gene="kefF"
                     /locus_tag="ACHOQW_002557"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP9573620.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(12472..13803)
                     /locus_tag="ACHOQW_002558"
     CDS             complement(12472..13803)
                     /locus_tag="ACHOQW_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9573621.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13861..14148)
                     /gene="fixX"
                     /locus_tag="ACHOQW_002559"
     CDS             complement(13861..14148)
                     /gene="fixX"
                     /locus_tag="ACHOQW_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP9573622.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(14145..15431)
                     /locus_tag="ACHOQW_002560"
     CDS             complement(14145..15431)
                     /locus_tag="ACHOQW_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9573623.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(15482..16423)
                     /locus_tag="ACHOQW_002561"
     CDS             complement(15482..16423)
                     /locus_tag="ACHOQW_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9573624.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(16438..17208)
                     /locus_tag="ACHOQW_002562"
     CDS             complement(16438..17208)
                     /locus_tag="ACHOQW_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP9573625.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         4230 a         4793 c         4294 g         3936 t
ORIGIN      
        1 aggttagcat tccacccagg ttccaagtaa aggactttat tgtgaccttt cattgttttg
       61 agaccaaggt aacaatccct catgtcaacg ccgggaaggt aagcctctct aagatcaacg
      121 cccaagttac ctgggccttt atgcagctgc aacgtctgag tacatatctc cttattttca
      181 ataaaaaaag tgacacttaa ctcgatatca ccgaaggtct gtgcgaccca gctcatattg
      241 ccctttgcga ccatctcatc cggatttttt gcgcatgttt tgagttcatt gaataattcc
      301 agactatgga taaacttttc tttctcattc tcgttgacag ccccagtaaa gtattttgag
      361 gcgttgatgt tgccagcctt tttattcacg aaattgaaca gaagtttata agcctcagtc
      421 tgtttatctg accagaacag atgacgtaac gtctcccaca agcgttgaaa gatattcatc
      481 ttcatgtcca tcttgtcccc gctcaccatc ttataaatat cgttattgct catatttaaa
      541 agtgtagccg gcatgcctgg aactgatact ttcatggtaa tagcctcgca ttgagttaaa
      601 aactcatgat gcgcgctctg aataaatata ctttcaaaaa tcgctcattc agatatgtgt
      661 tttttatttt taagacgtaa aaatataatt ccgctcgccg taaagctctc aaccttaagc
      721 agggtcaacg cgataaaacc aacagaaacc ctcccgtaaa ccgtatattg agcctgtcca
      781 ccattctgag taaacgcctt ttctcaaaga agtgaccgcc agggcggcca ccttttatcg
      841 tgggcagtta ctcagcatca tttacagggt catttaaacc ctttctgctg cttaatcagc
      901 tcataggcct gctgaatttc ctgcgctttc tgcttcgcca tctccatcat ctcaggcggc
      961 aaacctttcg ccaccagctt atcgggatgg tgttcactca tcagcttacg gtaggcacgt
     1021 ttgatggtgg tcgcatcatc cgtcggcttc acgcccagca cattacaggc atcttccagc
     1081 gttgggccac gctgcgcttg ctgccagtta ccaccgccag attgctgctg ataaccgccg
     1141 ccaaactgtg caccgccctg catcatgcgc aaaaactggt caaactgggc gcgggagatc
     1201 cctaattctt cagcaatgac atacagcacc gcccgttcat tcgggtgcag tgaaccatca
     1261 gcaaacgccg cctgaatctg gatctccaga aacatacgaa ttaagtcaaa acgaccaaag
     1321 cagacactgc gaaactggcg catcttttcg cgcagcgggt aattgtctga ttttcccacc
     1381 cggaacgcat tttgcgccgc agtacgggaa gcgccatgaa gattcattcg gtccatcaac
     1441 tggctggcga tatgaatatc agcctccgtg acgcgacctt tggatttggt taaatgcccc
     1501 atcacttcaa aagtggtggc aaaaaacagc gcctgacgct cacgctggtt ggcgaaccac
     1561 gccattttac ggctacgggc tttatcaaac atatggccaa ttaacaggcc taacactacg
     1621 ccccaaaagc cgccgcccat cagtaaggcc acggccacgc caatgatttt tccccaatac
     1681 tgcatatatt ccccaaatcg acacacggat atcagggcta tctcccacaa tataaaggtg
     1741 cttttaccgt tttccggctg cggtcaattg tgggacatcg cctataattt gcattatcat
     1801 acctgtcatt caatgccgtg cctaacacca ctgacgctat tcggacagga ttaacactag
     1861 cgtagagatg acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt taccgatgat
     1921 ggaacaataa aatcaacgta tgaaaaaacg tatccccact ctcctggcca ccatgattgc
     1981 caccgccctt tatagtcaac agggactggc agccgacctc gcctcacagt gcatgttggg
     2041 cgtgccaagc tatgaccgtc ctctggtaca gggcgatacc aatgacttac ccgtgactat
     2101 caatgctaac cacgcgaaag gggactaccc ggatgacgcc gtgtttactg gcagcgtgga
     2161 tatcatgcag ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc
     2221 accaggacaa ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc attacgacga
     2281 taaccaggtg atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca aagataccaa
     2341 cgtctgggaa ggtgattacc agatggtggg tcgccagggt cgcggtaaag cggacctgat
     2401 gaaacaacgt ggcgaaaacc gctataccat tctggataac ggtagcttta cctcctgtct
     2461 gccgggttct gacacctgga gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca
     2521 agttgcggag atctggaacg cccgctttaa ggtgggtccg gtatcgatct tttatagccc
     2581 ctatttgcag ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc cgaacgccaa
     2641 gtacaccacc accaactact ttgagttcta cctgccatat tactggaaca tcgcgccaaa
     2701 tatggatgcc accatcacgc cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa
     2761 cgaattccgc tacctctccc aggcgggcgc tggcttgatg gaactggact atctgccttc
     2821 agataaagtc tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt ggttattcta
     2881 ctggaaccac tccggggtca tggatcaggt gtggcgtttc aacgtcgact acaccaaggt
     2941 cagcgatcct agctacttca atgatttcga taacaagtac ggttccagta ctgacggcta
     3001 cgcaacgcaa aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca ccgtttcaac
     3061 caagcagttc caggttttca gcgaacagaa caccagtagc tactcggcag agccgcagtt
     3121 agacgttaat tactaccaga atgatgttgg tccgtttgat acgcgtattt acggccaggc
     3181 agtgcacttt gttaacacca gagacgacat gcctgaagca acccgtgttc acctggaacc
     3241 gaccatcaat ttgccgctct ctaataactg gggcagcatc aataccgaag cgaagttgct
     3301 ggcaacccat tatcagcaaa ccaatcttga ctggtataac tccagaaaca cgaccaagct
     3361 ggacgaatcc gttaaccgcg taatgccgca attcaaagtt gacggcaaaa tggtctttga
     3421 acgcgatatg gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta
     3481 tttgtacgtg ccgtatcgcg atcagagcga catctataac tacgactcgt ctctgctgca
     3541 atctgactac tctggcctgt tccgggaccg gacttacggc ggtcttgacc gtattgcctc
     3601 cgctaaccag gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg ccgttgaacg
     3661 ttttaatatt tccgttggtc aaatctacta tttcacggag tctcgcactg gcgatgacaa
     3721 cataacatgg gagaatgacg acaaaacggg ttcactggtg tgggcaggcg atacttactg
     3781 gcgtatctcc gagcgttggg gattgcgtgg cgggattcag tacgatacac gtctggataa
     3841 cgtagcgacc agtaactcca gcattgaata ccgtcgggat gaagaccgtc tggtacagct
     3901 gaattaccgt tacgccagcc cggaatatat tcaggctacg ctgcctaagt actattccac
     3961 tgctgagcaa tataagaatg gtatttcgca ggtaggtgct gtcgccagct ggccaattgc
     4021 cgatcgttgg tccattgttg gggcctacta ctacgacacc aatgcgaaca agcaagccga
     4081 ctctatgtta ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg gttacgagcg
     4141 gaagctgaac ggttgggata acgataaaca acatgcggta tatgacaacg caatcggctt
     4201 taacatcgaa cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag agatgctgcg
     4261 ttcgaacatt ctgccgtatc aaaacacttt gtgatttgtt gatttaccac gtaatccgca
     4321 gtgcggttaa ttgaaatgga aaaagtatga agaactggaa aacgctgctt ctcggtatcg
     4381 ccatgatcgc gaataccagt ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg
     4441 tcaataacgg cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga
     4501 acgctgctca ggcaaggcag caacttcctg atgacgcgac gctgcgccac caaatcatgg
     4561 aacgtttgat catggatcaa atcatcctgc agatggggca gaaaatggga gtgaaaatct
     4621 ccgatgagca gctggatcag gcgattgcta acatcgcgaa acagaacaac atgacgctgg
     4681 atcagatgcg cagccgtctg gcttacgatg gtctgaacta caacacctat cgtaaccaga
     4741 tccgcaaaga gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca
     4801 tcctgccgca ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac gacgccagca
     4861 ctgagctgaa cctgagccac atcctgatcc cgctgccgga aaacccgacc tctgatcagg
     4921 tgaacgaagc ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt
     4981 tcggtaagct ggcgattgct cattctgccg accagcaggc gctgaacggc ggccagatgg
     5041 gctggggccg tattcaggag ttgcccggga tcttcgccca ggcattaagc accgcgaaga
     5101 aaggcgacat tgttggcccg attcgttccg gcgttggctt ccatatactg aaagttaacg
     5161 acctgcgcgg cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc catattctgc
     5221 tgaaaccgtc gccgatcatg actgacgaac aggctcgtgt gaaactggaa cagattgctg
     5281 ctgatatcaa gagtggtaaa acgacttttg ctgccgcagc gaaagagttc tctcaggatc
     5341 caggctctgc taaccagggc ggtgatctcg gctgggctac accagatatt ttcgatccgg
     5401 ccttccgtga cgccctgacc cgcctgaaca aaggtcaaat gagcgcaccg gttcactctt
     5461 cattcggctg gcatttaatc gaactgctgg atacccgtaa tgtcgataaa accgacgctg
     5521 cgcagaaaga tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa
     5581 gctggatgca ggaacaacgt gccagcgcct acgttaaaat cctgagcaac taatggttaa
     5641 aacccaacgt gttgtgatca ctcccggcga gcccgccggg attggcccgg acttagttgt
     5701 ccagcttgca cagcgtgagt ggccggtcga actggttgtt tgtgccgatg ccactctcct
     5761 taccgaccgg gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt cccccaactc
     5821 ccctgcacaa ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc tacgtgaatc
     5881 tgtcactgcg gggcagttag cgattgaaaa tgggcattac gtggtggaga cgctggcgcg
     5941 agcgtgtgat ggctgtctga acggtgaatt tgctgcgctg atcacaggcc ccgtgcataa
     6001 aggcgtcatt aacgacgcag gcattccgtt taccggtcat accgagtttt tcgaagagcg
     6061 ttcgcaggcg aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc
     6121 aacgacgcat ttaccgctgc gcgatatcgc agatgctatc acccctgcac ttttgcacga
     6181 agtgattgct attttgcatc acgatttgcg tacaaaattt ggtattgccg aaccgcgcat
     6241 tctggtctgc gggctgaatc cgcacgcggg cgaaggcggt catatgggta cggaagagat
     6301 agacaccatt attcctgtgc tcgacgagct gcgggcgcag gggatgaaac tcaacgggcc
     6361 gctgcctgcc gataccctgt ttcagccgaa atatctcgat aacgccgacg ccgtgctggc
     6421 gatgtaccac gatcagggtc ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa
     6481 cattacgctg ggcctgccct ttattcgcac atcagtggac cacggcaccg cacttgaact
     6541 ggcgggacgt ggcgaagccg atgtcggcag ttttattacg gcgcttaatc tcgccatcaa
     6601 aatgattgtt aacacccaat gaataatcga gtccaccagg gccacttagc ccgtaaacgc
     6661 ttcgggcaaa actttctcaa cgatcagttc gtgatcgaca gcattgtttc tgccattaac
     6721 ccgcaaaagg gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt gaccgagccg
     6781 gtcggcgaac gtctggacca gctgacggtc atcgaacttg accgcgatct ggcggcacgt
     6841 ctgcaaacgc atccattctt aggcccgaaa ctgacgattt atcagcagga tgcgatgacc
     6901 tttaactttg gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg
     6961 ccttataaca tctccacgcc gttgatgttc catctgttta gctatactga tgccattgcc
     7021 gacatgcact ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg accgaacagc
     7081 aaagcgtatg gtcgattaag cgtcatggcg caatactatt gcaacgtgat cccggtactg
     7141 gaagttccgc cgtcagcctt tacaccacca cccaaagtgg attccgccgt cgtgcgcctg
     7201 gttcctcatg caacgatgcc tcacccggtt aaagatgttc gtgtgttgag ccgcatcacc
     7261 accgaagcct ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc
     7321 gtcgaggtgt taacgggaat ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc
     7381 gcgcaatatt gccagatggc gaactatctg gcggagaacg cgccttttca ggagagttaa
     7441 cgatgatcaa ttcgccccga gtgtgtattc aggttcaaag cgtctacatt gaggcacaat
     7501 cttcacctga taatgaacgt tacgtttttg cttataccgt aaccatacgc aatctggggc
     7561 gagcgccagt gcagttgttg gggcgttact ggctgatcac caatggcaat ggccgtgaaa
     7621 ccgaagtcca gggcgaagga gtggttggcg tccagccact tatcgcgcct ggcgaagagt
     7681 accagtacac cagcggtgca atcattgaaa ccccgctggg caccatgcag ggtcactacg
     7741 aaatgatcga tgaaaatggc gtccctttca gcatcgacat tcccgtattc cgactcgccg
     7801 ttcccacact cattcattaa aagaatatgg cgacatacct tattggcgac gttcatggtt
     7861 gttacgatga actgatcgca ttgctgcata aagtagaatt tacccctggg aaagataccc
     7921 tctggctgac gggcgatctg gtcgcgcgcg gcccgggttc gctggatgtt ctgcgctatg
     7981 tgaaatcctt aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg catctgctgg
     8041 cagtatttgc cgggatcagc cgcaataaac cgaaagatcg cctgacaccg ctgctggaag
     8101 cgccggatgc cgacgagctg cttaactggc tgcgtcgcca gcctctgctg caaatcgacg
     8161 aagagaaaaa gctggtgatg gcccacgcag ggatcacgcc gcagtgggat ctgcagaccg
     8221 ccaaagagtg cgcgcgcgat gtagaagcgg tgctgtcgag tgactcctat cccttctttc
     8281 tcgacgctat gtacggcgat atgccaaata actggtcacc ggaattgcgg gggctgggaa
     8341 gactgcggtt tatcaccaac gcctttaccc gtatgcgttt ttgcttcccg aacggtcaac
     8401 tggatatgta cagcaaagaa tcgccggaag aggcccctgc cccactgaaa ccgtggtttg
     8461 cgattcctgg acctgtcgct gaagaataca gcatcgcctt tggtcactgg gcatcgctgg
     8521 aaggcaaagg tacgccggaa ggtatttacg cgctggatac cggctgctgc tggggtgggt
     8581 cattaacctg cctgcgctgg gaagataaac agtattttgt ccagccgtcg aaccggcata
     8641 aggatttggg tgagggagag gcggtcgcgt cttaaatccc gtcgagcata aaatctaatg
     8701 ctcctttaat caacgttcgg tgcgcgctgg catcgcaaaa tttcgttccc agcgcgttaa
     8761 ctggaacagt tgcagtctca tgcgtcatga gcacatattc tttgccgtct accagtaaaa
     8821 ggattgggtt aagccgttct ggtgggcgaa tacggctaaa tcgttcaata ggtgttaatg
     8881 gaataacaat acggcgatta atcacaccaa taatgtcgct ggtaacatcg ataacaaagg
     8941 gaaacgcggc gttcctgctg cgactgcggt ataccgtaaa ttgcatcgct agaagctccg
     9001 gtactcatca gagaaacatc cggtttcatc agccaattga tttaacgcag cgatagcttc
     9061 agcgttctct tcacgccaac gtgttgctgc atgtttttta agttcagcat tgagcgcaac
     9121 cgtaagggtg gcgctcatat ttaagcctgc atcacgagcg cgattgacta actcacggtc
     9181 gacggtgacg gtcacacttt gtgtggtacg ttttgcagtc atgatatcgt gctcctgtac
     9241 aatttatacg tctagtatac acggatatct gcacgtatca gtgatgccgg aattctaata
     9301 tacaaaatta ccgccgctcc agaatctcaa aacaatagct gtgagagttc tgcgcatcag
     9361 cgtcgtggaa ttcgctgaat accgattccc agtcatccgg ctcgtaatcc ggaaaatggg
     9421 tgtcgccttc cacttctgcg tcgatatgcg tcaggtacag tttttgcgct ttcggcagga
     9481 actgctcata aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg
     9541 cgatggcttc atccaccgac ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc
     9601 tgaggataat atttttgcgt cctggcaacg gacgaccgat ggattccctg gtatggcggc
     9661 ccataattac gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt
     9721 tccacggcat ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac
     9781 tgatcattga gatttcccga taaaaaaaat tgtcgccact atacgtaaag cgtaaaccgt
     9841 cgtcgactgg tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc
     9901 tctctccctg gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt
     9961 gatttacgtc actctttatt aggatgaggg tttcgtttcc ggttcatccg ccatgttgcc
    10021 ggtatgttta ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc
    10081 gcggtcctcg gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg
    10141 ggctttggtg tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg
    10201 gaacacatcg gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag
    10261 acgcccggtt ttcagcgcac cttcaaaggt ttcacgctct ggcttttcaa cgcctgcctg
    10321 acgcaaacaa atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa
    10381 atgttctttc accatctccg tcagttgcag gttggtttgc ggatcgtcaa tggcgttaat
    10441 cagcacttcc gctttcgccg ctcccgcaga ttccagtaaa tccatccgcg tggcatcgcc
    10501 ataaaacact ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag
    10561 caccaccatt ttcaccccgc tggagagcag taaacgtccg gtgatctgcc cgaaacgacc
    10621 gaatccggca atgattaccc gcggctgttc ttcgtcgatc tcatcggctt cacgcgcctc
    10681 ttcagtagaa gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgccgccat
    10741 cgacagcgcc accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat
    10801 ctgcgccgcg ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa
    10861 ccagcgacgc tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat
    10921 tttgatgatg aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt
    10981 gccaaagtct atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa
    11041 gggttcgata tcgctttcca gcgcatgacg gtattccgag cttgccagca gtacgcccgc
    11101 cagaaacgcg cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa
    11161 cacaaggaat aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg
    11221 cagcgccgga cgcgtgacat agcgccccag cagtaccacc agcaccagcg cgcccgccac
    11281 ttttaacgcc gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag
    11341 cgggatcatc gccaccagcg ggattgccgc gatatcctgg aacagcagca ccgcaaaggc
    11401 actgcggccc atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc
    11461 cgtagaggag agcgccagcg tcatgccgat caattccgcg acctgccagc gcagcccaag
    11521 taacatacag aacagcccca gcaggccgcc gcaaatcacc atctgcaatg cgccaccgcc
    11581 aaacactgcc gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa
    11641 cagcatcagc accacgccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag
    11701 tcgcagcccc cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag
    11761 accaagacgt actgcaatgg gtacaatcag cgccgccgaa ccgagataaa tcagcgcctg
    11821 aaccagcgta tggctatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt
    11881 gacgcgcctg cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg
    11941 gtggcagcca gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat
    12001 caaagcccgg atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc
    12061 acagcaaatg tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaga
    12121 agactttatc gatccaaagt ttaaggagcg gaggaatgct gtaccactgc atcgggtgct
    12181 gccagacgat cagatcggcg cgagacagcg cctcctgctc ggcggcaata tcgatattga
    12241 agtcaggata gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt
    12301 caaacatccg tttattcgca tgggaatgat gcggatacgg atgcgcataa attataagaa
    12361 tcataggtag cccgcgtgtt tacgcctttg ttttcccaca aagtgtagtc atatattcac
    12421 gcggcttata gtcaataata ttgattcaga aaagagataa atctgaacaa attaccccat
    12481 tctctgcccg cggatcgtca tattgctggt ctgcgccagt gacatccctc gagtctccgg
    12541 ggcaaacgct acggaaatca acaagccaaa cagcgagata cccgccccca ttagcatcgt
    12601 gttactgata ccgtaattat tgataaagat cggtagtgcc caggtcgaaa caatggtgcc
    12661 aatacgactt aaggacataa tcacgcccac ggcagaggcg cggatatccg tcgggaagag
    12721 ttcattagga tagagccact gcaaattacc cggcccgcca gagaaaaagg catacaccgc
    12781 aaaggccatc actaccagcc agatccccat atccgggatc agccccaaaa ccgccagcgc
    12841 cagcgtcatc atggcaaagc tgccaatcaa caatggacgc cgtccggcag tgtttagcca
    12901 cagcatcggc ggaatacagc cgagcataaa gaacaggcta atcaccacgt tccccagtgc
    12961 cgcgtttttg ccaacaccca atcccaacaa accaacgatt tgcgggccaa aggtgtaaat
    13021 ggcgaacatt gggatcacct ggcaggtcca gatggcggca acaaacagaa caaaaggaaa
    13081 atggcggcga ttaaacagat cgcgaaaacg ggtttgctgc ggctgctctt catcgaaagc
    13141 caccggttcg ccaaacagtt tgatcatcat ctcttcgcac tcttttactc gccctttgcg
    13201 taataaccag cggggagatt caggcagttc gaatcgacca atcaaaatca acaaacaggg
    13261 gatcgccgcg ctacccagca tccagcgcca gccgccttcc acatcataaa gccagtagcc
    13321 gaccagatcg gcacaggtcg cgccgacata ccacatggcg gcgataaagc tgatggaaaa
    13381 cgcccgctga cgggtactgg agaactcagt gatcatcgaa gtggcgatgg gatagtctgc
    13441 accgatgaca atgccgataa gtacccgcat caccaacagt tcgacggggg atgaaacaaa
    13501 catcgtcgcc accgatatca cgccgatggc gatgatatca atgaggaaca ttttgcgccg
    13561 tccgacttta tcggaaatat aaccaaacag cgatgtgcca acgaacagcc cggcgagcgt
    13621 tcccgcgccc agcaagccaa tccagtcagc gtccagtttt agcgccggcg tcagttgctc
    13681 cagcgccacg ccaatcatta ccagtatata accatccaga aacggaccac cgcttcccca
    13741 cagcaaaatg cggcggtgaa tagaggaaaa tttaagatcg tcaaagtttc tggacggttg
    13801 catgggttat tcctgttttt tgcaatgaga gtcagtgcgg cgttacgtat caaaccaaca
    13861 tcagccgtaa cggaactcca caccaaaggt gccgcgcggg tattcccact gttccagcgc
    13921 cgaccccagc cccagaatgc gacaggtgcc gcactccaga catccggcgt aatcgaagcg
    13981 cacactgccg tcatcctgct tcttgtacag acctgcgggg cacgctttca ccagcagctc
    14041 cagcgcctgt ttatcggcat cggctttcac aacaatgtgc ggatgctctt catcgacatt
    14101 gaatttattg acgcccagtt tgacgtccac attgacggga gaagtcataa aacggtcact
    14161 cctttcatgc catccttgat cagattgatg aagcccactt tcttgccgtg gcggaggatt
    14221 ttcttgcgca tcagttccgg cgcactgccg tcaatggtga acaggtcacg cgccacgcct
    14281 accgccagtt ccgggtagcc gctaaacatg cgtgggttat caaggaaggc cggtagtttc
    14341 tggtacatac gcatatcgcg cagcgggcca ctctcaagat gctgacgata ttccgcgagt
    14401 ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca cggtttttgc tgcagcttcc
    14461 ccggcggcaa tcgccagatc cataccgcga atggtaaaac cgaggttcat acacattccg
    14521 gcggcatcac cggcaatcaa tacgccgtca ccgaccagct ccggcagcat gttgatgcct
    14581 gcttccggca ctacgtgagc ggaatattcc accagcttgc cacccgcgat cagcggtgca
    14641 acggccggat gttgtttgaa atcttccagc atttgcggca ccgatttttt cgcgtcatgc
    14701 agatgatgca gaccacaaac cagccccagc gacagggtgt tttcattggt ataaagaaaa
    14761 ccgccgccca tcaggccatc ggtgggtgat cccgcaaata ggcaagccgc cccctgatta
    14821 ccctgcaact gaaaacggtc ttcaataacc gacttcggta actcgatcag ttccttcacg
    14881 ccaaccgcca cattcgtcgg tttaacgcgt tttgccatcc ccagcttttc ggcaaggatg
    14941 gagttcactc catcagcaag gatcaccgtt ttcgcttcaa tcacatcgcc atcggcttct
    15001 acaccgacga ctttgccatc gcgctgtacg aggttatcga cgcggatccc ggtaattaac
    15061 tgcgcgcccg cttcttcggc ctgctccatc agccaggcat caaatttact gcgcaaaacg
    15121 gagtaagaac gctgggatgg cgaggtttcg tcaccgttgc agtagtccat agtcatcgct
    15181 gacttttccg tcataaacgc gagtttttca tgggtgatca ggcgttctac cggggcggat
    15241 tcggcgaaac caggaataat gtgttccagg ctgtgggcat agagacgccc gccggtgacg
    15301 ttcttggcac ctgcggaatt gccacgctcg ataactaaca cttgcgcccc ttcgcgggcg
    15361 agcaccagtg cggcaaccga accggcaagc cctgcaccga cgatgatggc gtcaaagata
    15421 tcttcggaca taagagctcc ctgtcagcgg caggaccaaa atgcagccct gccagagtgg
    15481 atcaacgcgc taaagctgcg gtcagcgccg gaaggatctt cacggcgtcg ccaacaatgc
    15541 cgtaatccgc gtactggaag atcggcgcat ttttatcttt attgatggcg aaaatggttt
    15601 gcgacgcgtt agcgccaacc atgtgctgga tctgcccgga gatccccaca gccaggtaca
    15661 gttcaggttt cagcatcagg ttggagatac cgacatagcg ttcgtgctcc atccattttt
    15721 cgttttctgc caccggacga gaacaggcca actccgcacc tatcgccttg caaagctgtt
    15781 ctgccagcgc aatgttctct ttgctgccaa taccgcgacc gacgctgacc accaggcggg
    15841 ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt tgccgtgcgg gtaatcgcca
    15901 cagccggagc ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg tctggctgag
    15961 ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata cggcgtggca atgcgttctt
    16021 cgccaatcgc cagaccaccg taaaccatat gtttcactgt tgctttaccg tcctgtacgc
    16081 tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg ataacccagt tttgccgcca
    16141 gtaatttgcc gcgacgggtg tttggcaaca gcaccaggcc gtctgcaccg tgctggcgaa
    16201 tagtgtcagc catgacactg gcgtaatctt cgatcatccg atcgtccggt ttgccgttta
    16261 atttccagac atgattagcg ccgagctgga ttgcctgtgc gccgtcggca tcattgagga
    16321 caaaggcatt gatttgatta gctaaagcct gcgcaccgtt catcagttcc ggcagacgag
    16381 aaggggtatc gctgaatacc cagacttgag aaaacgtgtt catagcatcc cctgtaatta
    16441 aatgactttg cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt cgccttcaat
    16501 cacgatgcgc tgacgctcgc gctgtttcgg cgcggcaacc tgttgttctg accatgctgc
    16561 ctctgcgtta aaaccgatat ccgccgccga ccatacctgg actggctttt tcgccgcgcc
    16621 gagaatggct ttcatcgaag gaatttgtgg ggagttgata tcagtggaaa cagcaacaac
    16681 cgcaggcagt ggaatgctta aggtttcggt ttcatcttcc agttcgcgct caacggtgag
    16741 ggtatctgcc gtcagggaga taattttgct gacgccgtta actgccggaa tattgaggat
    16801 ttcgcctacc agcaaaccaa cctgctgggc ataaaggtcg gaagaaccat cgccacagag
    16861 gatcaaatca aagcctgctt tctgggcggc tgcagccagt gcgctcgccg tttgttgcgg
    16921 cagtgcctgc tcgaactggt catcaatcac cacaatcagt tcatccggac cgcgcgatag
    16981 cacatcttta cgccctttgg cgttggtcag ggctttaccg cccacactta gggccgtcac
    17041 ctgcgcctct gctgcctgtt gctttagctg gcaagccgct tcaatagcgt tgagatcgta
    17101 ttggcttatt ttggcatcgg ctttactgaa gtctaacgaa ccatcagcat tattgaccgc
    17161 aatatcctgt tcatcaggca cgcacttata gcaagtaata atcttcattg catctccaga
    17221 aatcatgaag gaaacgtcgt tcatatcggt ggt
//
LOCUS       DAZKFZ010000084        17203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000084 DAZKFZ010000000
VERSION     DAZKFZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            272..1615
                     /locus_tag="ACHOQW_002563"
     CDS             272..1615
                     /locus_tag="ACHOQW_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573626.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1764..3272)
                     /gene="putP"
                     /locus_tag="ACHOQW_002564"
     CDS             complement(1764..3272)
                     /gene="putP"
                     /locus_tag="ACHOQW_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP9573627.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3431..3640)
                     /locus_tag="ACHOQW_002565"
     CDS             complement(3431..3640)
                     /locus_tag="ACHOQW_002565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573628.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3695..7657
                     /gene="putA"
                     /locus_tag="ACHOQW_002566"
     CDS             3695..7657
                     /gene="putA"
                     /locus_tag="ACHOQW_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9573629.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7697..8335)
                     /gene="rutR"
                     /locus_tag="ACHOQW_002567"
     CDS             complement(7697..8335)
                     /gene="rutR"
                     /locus_tag="ACHOQW_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP9573630.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8623..9714
                     /gene="rutA"
                     /locus_tag="ACHOQW_002568"
     CDS             8623..9714
                     /gene="rutA"
                     /locus_tag="ACHOQW_002568"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP9573631.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9714..10406
                     /gene="rutB"
                     /locus_tag="ACHOQW_002569"
     CDS             9714..10406
                     /gene="rutB"
                     /locus_tag="ACHOQW_002569"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP9573632.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10418..10804
                     /gene="rutC"
                     /locus_tag="ACHOQW_002570"
     CDS             10418..10804
                     /gene="rutC"
                     /locus_tag="ACHOQW_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP9573633.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10812..11612
                     /gene="rutD"
                     /locus_tag="ACHOQW_002571"
     CDS             10812..11612
                     /gene="rutD"
                     /locus_tag="ACHOQW_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP9573634.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTSNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11622..12212
                     /gene="rutE"
                     /locus_tag="ACHOQW_002572"
     CDS             11622..12212
                     /gene="rutE"
                     /locus_tag="ACHOQW_002572"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP9573635.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12223..12717
                     /gene="rutF"
                     /locus_tag="ACHOQW_002573"
     CDS             12223..12717
                     /gene="rutF"
                     /locus_tag="ACHOQW_002573"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP9573636.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12738..14066
                     /gene="rutG"
                     /locus_tag="ACHOQW_002574"
     CDS             12738..14066
                     /gene="rutG"
                     /locus_tag="ACHOQW_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP9573637.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14149..14256)
                     /locus_tag="ACHOQW_002575"
     CDS             complement(14149..14256)
                     /locus_tag="ACHOQW_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFP9573638.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            14628..15224
                     /gene="wrbA"
                     /locus_tag="ACHOQW_002576"
     CDS             14628..15224
                     /gene="wrbA"
                     /locus_tag="ACHOQW_002576"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9573639.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15245..15472
                     /gene="yccJ"
                     /locus_tag="ACHOQW_002577"
     CDS             15245..15472
                     /gene="yccJ"
                     /locus_tag="ACHOQW_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP9573640.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15510..16751)
                     /gene="agp"
                     /locus_tag="ACHOQW_002578"
     CDS             complement(15510..16751)
                     /gene="agp"
                     /locus_tag="ACHOQW_002578"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP9573641.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(17042..>17203)
                     /locus_tag="ACHOQW_002579"
     CDS             complement(17042..>17203)
                     /locus_tag="ACHOQW_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573642.1"
                     /translation="ESFSTDEDLEYGYLINTGNHYDVYLPPELFAQAYELNNKERNAQ
                     IGFLTRYAI"
BASE COUNT         4093 a         4578 c         4540 g         3992 t
ORIGIN      
        1 tattatttaa cgcgatgata aaaggcgtaa aaaaaaataa taatcttcat gaaactcacc
       61 ccgatggcag cattgtaaat tcgccaataa ctggcattcc tcctgctttt tacgggtgtt
      121 tccattaaca ccagaatgag cggattgttt actacatcaa gaacgcatcc gaagtgtaca
      181 tcctggtgcg ccgactgaat cgcccggagc catgcggatg gctacgactt aatacgaatc
      241 attaaccctg taaggatacg gagagcatta catgagacca atatccaacc tcaatacgag
      301 cgcagcaacc tatattccac cgcaacaact cccgagtagt tgtctggaaa ctttgacgct
      361 tctggtgcag atgaataatt atatcaaaag ggatgcggac tacagcacgg gcatggctgt
      421 agctccactg gttcctgaag aggttcactt gctggctgct gcgatgacaa ccgaactgca
      481 tcgacatcag ttccaaccgg tggtaagtgc aaatgatctg caacttcctg aaccgttcac
      541 cttcgatgtt gccggtttca ccatcacctt cacgaagacg caagagcatg acaacacggg
      601 caatctcatg aaaattgcag tcagcaagag cggtgtgtgc accagtacaa atattactct
      661 tgagctgttt cactccattg tcacaaccct gatgtcacgc agtcaatatg gaacctttga
      721 tctctggtcc gtacggccaa tactcacgga tgaaagtcaa aacagggtgc atgaagcagc
      781 tcgctactcc ccggcccagc aatacggaag agaagaaaca cactttacca acaatggcat
      841 gcgagaattt ggaaatatgt ctccgctggc gtggcgtaac gatgtggaat tacagcaatc
      901 gtgtaataca agcaacatcc caccaaatgc tgcaaatgac gacataaaca acaccaggca
      961 gaccatcgag gaccagccag aggcggacga gccacagcaa tatgttaaat tgactgtaga
     1021 cgacatgcga aaatgggcgg ctatggacca gcaggcgcga aacgctctac agggagtttc
     1081 aggatggtgt acgcgcaatc attttaatat caaaaatgcc agaaattacc tgaccgatca
     1141 cggcctgaac tacgcgggac aggtaaaggt aaacaggccg catgaatatg ccaaattcac
     1201 ccttgaacat atccgccagt gggcagcctt aaacaagcat gtgcgaaaac cggttggtta
     1261 tttagaaaag tggtgtaagg aaagaaacct cgcgccaact actgccagga attacctgaa
     1321 gaacgacggg cttaccgccc tgggtgagtt gaagctgaca gggccgcaga aatgggtcac
     1381 gttcaccttc ggagacatcg tacaatgggc gaatatgacc caggaggagc gtaacagcgc
     1441 agggggagcc gaaaagtgga gtaaaaaacg cggcttccag tggtctactg ccagatccta
     1501 cctgaaatcg agcggagtga ccaagcaagg tgcgagaaaa ctggcgtggt taaaaaacag
     1561 cgggaatatg tcgaatccat tctatctcgc ccaaccaacc agccgcagga cataagctga
     1621 aggtaccgat agcggcaatt ttgttcatct gtgccgggtg ggcaaatagc aattgccccc
     1681 gaacactccc tgccaggcaa cttataccgc atggcaggga acattcgggt taaaaagcaa
     1741 aagataacta attactcttc agcttagcct tcctgcaacc gtgacggcgg agccgaatga
     1801 tagtgcgcat cggcctcggc aaagcgtttt tgcatcgccg ctgacggtgc tttacccagc
     1861 aaactaaaca ctacaatccc aatactgcca aagataaagc ccggaataat ttcgtacagt
     1921 cccagccagc cgaactgttt ccagacgatc accgtcagcg caccgatgat catccccgcc
     1981 agcgcaccgt tgcgcgtcat gcgtgaccac atcaccgaaa acagcaccac cggaccaaac
     2041 gccgcgccaa agcctgccca cgcgtagctc actaagccca gcacgcggtt ttccgggttc
     2101 gctgccaacg caatcgccac cagcgccacc accagcacca tcacacgccc tacccacacc
     2161 agttctttct ggctggcatg tttacgcaga aacgctttgt acaaatcttc ggtaatcgca
     2221 ctggaacata ccagcagctg gcaacttaac gttgacatta ccgccgccag aatcgccgac
     2281 agcagaatcc cggcaatcca cgggttaaac agaatttgcg ccagttcgat aaacacgcgc
     2341 tcggcgttct ggtttaccgc accagctacc gacgggtgct cgttaaagta agcgatacca
     2401 aagaagccga cagccaccgc ccctgccagg cagaggatca tccaggtcat actgatacga
     2461 cgcgcatgga cgatgctgtg gtgagaatcc gccgccataa aacgcgccag gatgtgcggt
     2521 tgcccgaagt aacccagccc ccaccccatc agcgagataa tggcgacaaa gttcagccct
     2581 ttgagcatat ccacgttttc gatgctcttt tgtttgatca cttccagcga gtcaccaaag
     2641 ccaccgacac tgataatgac gataaccggc gtcaggatca gggcaaaaat catcaggctg
     2701 gcctgtacag tgtcagtcca gctcaccgcg aggaaaccgc caataaaggt gtaaaggatc
     2761 gtcgccgcag ctcccgccca cagagccgtt tcgtagctca tgccaaaggt actttcaaac
     2821 agacgcgcgc ctgccacaat gcccgaagcg caataaatgg tgaagaacag caaaatcacc
     2881 agcgcggaga taatgcgcaa aatgcggctt ttatcttcaa aacgcccggt gaaataatcc
     2941 ggtaatgtta aggcgttatt gttgtattcg gtatgcacac gcaaccgccc ggccaccagc
     3001 ttccagttaa tccacgcgcc taatgtcagg ccaatggcga tccaactttc ggaaatcccg
     3061 gaaagaaaaa ccgcgcccgg caaccccatt aacagccagc cgctcatatc cgatgcaccc
     3121 gccgataatg ccgtcacgaa tggcccaagg ctacgtccgc ccagaatata gtcgtcaaag
     3181 tttttcgttg atcgccaggc gataaaccca atcaatatca tgccaaagat atagacacaa
     3241 aatgtcacca acatcggtgt gctaatagcc atctaaagtc tccaaaaaat tattatcggc
     3301 aatgtcgaaa cttgccgtta tatctgccac cggaacgggg taacagagtt tatgttttac
     3361 cagggcgacc gtatcctgcc ggaagcgctg gttattcaca atcgatttaa cacaccattt
     3421 acattaaatt ttagtgctca gcgacactat ttttcatcag gttgcactct ctcacatttt
     3481 ttgcggttgc acctttcaaa aatgttaact gccgcagaga aaaagtctga gttatttttt
     3541 taatccctgt catatcgatt tcttttatta acatttcatt catttttaag cttgctacgc
     3601 aggtcacatt taacatggtt gcacaaagtt gcaacatcat ggatatttca cgataacgtt
     3661 aagttgcacc tttcagaaca acaggagtaa tggcatggga accaccacca tgggggttaa
     3721 gctggacgac gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg atcgcacgcc
     3781 acactggtta attaagcagg cgattttttc ttatctcgaa caactggaaa acagcgatac
     3841 tctgccagag ctacctgcgc tgctttctgg cgctgccaat gagagcgatg aagcaccgac
     3901 tccggcagag gaaccacacc agccattcct cgactttgcc gagcaaattt tgccccagtc
     3961 ggtttcccgc gctgcgatca ccgcggccta tcgccgcccg gaaaccgaag cggtttctat
     4021 gctgctggaa caagcccgcc tgccgcaacc agttgctgaa caggcacaca aactggcgta
     4081 tcaactggcc gataaactgc gtaatcaaaa aaatgccagt ggccgcgcag gtatggtcca
     4141 ggggttattg caggagtttt cgctgtcatc gcaggaaggc gtggcgctga tgtgtctggc
     4201 agaagcgttg ttgcgtattc ccgacaaagc cacccgtgat gcgttaattc gcgacaaaat
     4261 cagcaacggt aactggcagt cacacattgg tcgtagcccg tcactgtttg ttaatgccgc
     4321 tacctggggg ctgttgttta ctggcaaact ggtttccacc cataacgaag ccagcctctc
     4381 ccgctcgctg aaccgcatta tcggtaagag cggtgaaccg ctgatccgca aaggtgtgga
     4441 tatggcaatg cgcctgatgg gcgagcagtt cgtcacgggc gaaaccatcg cggaagcgtt
     4501 agccaatgcc cgcaagctgg aagagaaagg tttccgttac tcttacgata tgctcggcga
     4561 agctgcactg actgccgcag acgcacaggc gtacatggtt tcctatcagc aggcgattca
     4621 cgccattggt aaagcgtcta acggtcgtgg catctatgaa ggtccgggca tttcaatcaa
     4681 gctgtcggcg ctgcatccgc gttacagccg cgcccagtat gaccgggtaa tggaagagct
     4741 ttacccgcgt ctgaaatcac tcaccctgct ggcgcgtcag tacgatattg gtatcaacat
     4801 tgacgccgaa gaggccgatc gtctggagat ctccctcgat ctgctggaaa aactctgttt
     4861 cgagccagag ctggcaggct ggaacggcat cggttttgtt attcaggctt atcaaaaacg
     4921 ctgcccgctg gtgattgatt acctgattga tctcgccacc cgcagccgtc gccgtcttat
     4981 gattcgcctg gtgaaaggcg cgtactggga tagtgaaatc aagcgcgcgc agatggacgg
     5041 ccttgaaggt tatccggttt atacccgcaa ggtatatacc gacgtttctt atctcgcctg
     5101 tgcgaaaaag ctgctggcgg tgccgaatct aatctacccg cagttcgcaa cgcacaacgc
     5161 ccacacgctg gcggcgattt atcaactggc aggtcagaac tactatccgg gtcagtacga
     5221 gttccagtgc ctgcatggta tgggcgagcc gctgtatgag caggtcaccg ggaaagttgc
     5281 cgacggcaaa cttaaccgtc cgtgtcgtat ttatgccccg gtcggcacac atgaaacgct
     5341 gctggcgtat ctggtgcgcc gcctgctgga aaacggtgct aacacctcgt ttgttaaccg
     5401 tattgccgat acctctttgc cactggatga actggtcgcc gatccggtca ctgctgtaga
     5461 aaaactggca caacaggaag ggcaaactgg attaccgcat ccgaaaattc ccctgccgcg
     5521 cgatctttac ggtcacgggc gcgacaactc ggcagggctg gatctcgcta acgaacaccg
     5581 tctggcatcc ctctcttctg ccctgctcaa tagtgcactg caaaaatggc aggccttgcc
     5641 aatgctggaa caaccggtag cggcaggtga gatgtcgccc gttattaacc cggcggaacc
     5701 gaaagatatt gtgggctatg tacgtgaagc cacgccgcgt gaagtagagc aggcgctgga
     5761 aagtgcggtt aataacgcgc caatctggtt tgccactcct ccggctgaac gcgcggcgat
     5821 tttgcatcgc gctgccgtgc tgatggaaag ccagatgcag caactgattg gtattctggt
     5881 gcgtgaagcc ggaaaaacct tcagtaacgc cattgccgaa gtgcgtgaag cggtcgattt
     5941 tctccactac tacgccggac aggtgcggga tgatttcgct aacgaaaccc atcgtccatt
     6001 agggcctgtg gtatgtatca gcccgtggaa cttcccgctg gcgattttca ccgggcagat
     6061 cgctgccgca ctggcggcag gtaacagcgt actggcaaaa ccggcagaac aaacgccact
     6121 gattgccgcg caagggatcg ccattttgct ggaagcgggc gtaccgccag gcgtggtgca
     6181 attgctgcca ggtcggggtg aaaccgtggg cgcgcaactg acgggtgatg atcgcgtacg
     6241 cggggtgatg tttaccggtt caaccgaagt cgctacgtta ctgcagcgca atatcgccag
     6301 ccgcctcgac gctcagggtc gccctattcc gctcatcgct gaaaccggcg gcatgaacgc
     6361 gatgattgtc gattcttcag cactgaccga acaagtcgta atagatgtgc tggcctcggc
     6421 gttcgacagt gcgggtcagc gttgttcggc gctgcgcgtg ctgtgcctgc aagatgagat
     6481 tgccgaccac acgttgaaaa tgctgcgcgg cgcaatggcc gaatgccgga tgggtaatcc
     6541 gggtcgcctg accaccgata tcggtccggt gattgatagc gaagcgaaag ccaatattga
     6601 gcgccatatt cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg tgcgggaaaa
     6661 cagcgaagat gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc tgatcgaact
     6721 ggatgacttt gccgaattgc aaaaagaggt ctttggtccg gtgctgcatg tggtgcgtta
     6781 taaccgtaat cagctgccgg agctgatcga gcagattaac gcttccggct atggtctgac
     6841 gcttggcgtt catacgcgta ttgatgaaac catcgcccag gtcactggct cagcccatgt
     6901 tggtaacctg tatgttaacc gtaatatggt gggcgcagtg gttggggtgc agccattcgg
     6961 cggcgaaggg ttgtccggta ccgggccgaa agcaggcggt ccgctctatc tctaccgtct
     7021 gctggcgaat cgcccggaaa gtgcgctggc ggtgacactc gcgcgtcagg atgcagagta
     7081 tccggtcgat gcgcagttga aagccgcatt gactcagccg ctaaatgcac tgcgggaatg
     7141 ggcagcaaat cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc tggcgcaggc
     7201 aggaacacaa cgattgctgc cagggccgac gggtgagcgc aacacctgga cgctgctgcc
     7261 gcgtgagcgc gtattgtgta tcgcggatga tgaacaggat gcgctgactc agctcgccgc
     7321 cgtgctggcg gtgggcagcc aggtgctgtg gccagatgac gcgctgcatc gtcagttagt
     7381 gaaggcattg ccatcgactg tcagcgagcg tattcaactg gcgaaagcgg aaaatattac
     7441 cgctcaaccg tttgatgcgg tgatcttcca cggtgattcg gatcagcttc gcgcattgtg
     7501 tgaagcagtt gccgcgcggg atggcgcaat tgtttcggtg cagggttttg cccgtggcga
     7561 aagcaatatc ctgctggaac ggctgtatat cgagcgttcg ctgagtgtga ataccgctgc
     7621 cgctggcggt aacgccagct taatgacgat cggttaaaca ggtagccgga ggatgttaca
     7681 ccctcctccg gcatcattag cgtggtcgaa tcccctcaat aataatccgc tgcacgtttt
     7741 caaccgtttg attgaaaaat acctcatcgc gcaacgttgc gcctgtcacc gcctccacct
     7801 gaggggcgaa atcggcgtaa tgttgagtgg aagcccaaat cataaaaatc aaatgctgcg
     7861 gatcaatcgg cgcgagtttg ccgcttttga cccaaccggc aatcagcgcc gatttctcat
     7921 caattaatga cttcaaatcg cccgtcagtt catccattaa cagcggcgcg cctgccagca
     7981 tctccataca gaacaggcgc gaagcctgcg gataatcgcg tgagacttcc agcttcagac
     8041 ggatgtactc tttgatcgcc gccagcgggg cgaaatcttc acgaaacgct tttaacggtg
     8101 ccagccagat atcgagaatc tgccgcagca cggcaatata cagcgcctct tttgacggaa
     8161 agtaatacag cagattggtt tttgaaacac ccgccaactc tgcaatttgc tccagccgtg
     8221 tgccgtgaaa accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc gcttttttct
     8281 tcgcgcttac tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc gtcatgcgct
     8341 ctcccctctt gtagattggc aacaggatag caaaaccccc gcccggcctc gaccttctgc
     8401 actctcatcg cgcacagatg catgttttat gtgcaactgt tttgaccgtt tagtccactt
     8461 tttaccagac attttagata acgcgatgtt aacacattaa taacactccc tttttaaaaa
     8521 ctggcatccg ctttgcaaac aagccaatac gcggctgaaa aggatatgca gaatgcagcg
     8581 ccccgtctga ctttcacttt acgagacgaa gagaggttga tgatgaaaat tggcgtattc
     8641 gtacctattg gcaacaacgg ctggctcatt tcgacccacg cgccgcagta catgccgacc
     8701 tttgaactga ataaagccat cgtgcaaaaa gcggagcact accatttcga tttcgccctg
     8761 tcgatgatca aactgcgtgg ctttggcggc aaaactgagt tctgggatca caaccttgag
     8821 tcgttcacct tgatggcggg gctggcggcc gtgacctcgc gcattcagat ttacgccacc
     8881 gccgccacct taacgttacc tccggcaatc gtcgcccgta tggccgcaac catcgactcc
     8941 atctctggcg ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc cgagtatgag
     9001 cagatgggta tctggcctgg cgatgactat ttctcccgtc gttacgacta tctcaccgag
     9061 tatgttcagg tgctgcgcga cctgtggggc acgggaaaaa gcgattttaa aggcgatttt
     9121 ttcaccatga atgattgtcg cgtcagtccg caaccgagtg tccccatgaa agtgatctgc
     9181 gccgggcaaa gcgacgctgg catggcgttc tccgctcagt atgccgattt caacttctgt
     9241 ttcggcaaag gcgtaaatac acccacggct ttcgccccga ccgctgcgcg gatgaaacag
     9301 gccgcagagc aaaccgggcg cgacgttggc tcttatgtat tgtttatggt gattgccgat
     9361 gaaaccgacg atgccgctcg cgccaaatgg gaacactaca aagcgggcgc ggatgaagag
     9421 gcgttaagct ggctaaccga acaaagtcag aaagataccc gctccggtac tgacaccaac
     9481 gtccgtcaga tggccgatcc cacttcggcg gtaaacatca atatggggac gttagtcggt
     9541 tcttacgcca gtgtcgcgcg catgttagat gaagtcgcaa gcgtgcctgg tgccgaaggc
     9601 gtgctgttaa ccttcgacga ttttctgtcg ggaatcgaaa ccttcggcga gcgcattcaa
     9661 ccactgatgc agtgccgcgc ccatctccct gtgctgactc aggaggtggc atgatgacga
     9721 cattaaccgc tcgaccggaa gccattacct tcgatccgca gcaaagtgcg ctgatcgtgg
     9781 tggatatgca aaacgcctat gccacgccag gcggctactt agatctcgcc gggtttgatg
     9841 tctcaaccac tcgcccggtc attgccaaca ttcaaaccgc cgtgaccgca gcgcgagcgg
     9901 cagggatgtt gatcatctgg tttcaaaatg gctgggatga acagtatgtc gaggctggcg
     9961 gccctggctc accgaatttt cataaatcga acgccctcaa aaccatgcgt aaacagccgc
    10021 agctgcaggg gaaattgctg gcgaaaggct cctgggatta tcaactggtg gatgaactgg
    10081 tgccgcagcc tggcgatatt gtgctgccga agccgcgcta cagcggtttc ttcaatacgc
    10141 cgctggacag cattttgcgc agccgcggaa tacgccatct ggttttcacc agcatcgcta
    10201 ccaacgtctg cgtcgaatcg acgctacgcg atggcttttt tctggagtat ttcggcgtgg
    10261 tgctggaaga cgcaacacac caggcggggc cggaatttgt acagaaagcc gcgttgttca
    10321 atatcgaaac cttttttggc tgggtcagcg acgtcgaaac gttctgcgac gcgctttctc
    10381 ccacgtcctt tgctcgtatc gcttaaggag tttaacgatg ccaaaatccg taattattcc
    10441 cgctggcagc agcgcaccgc tggccccctt cgttcccggc acgctggctg atggcgtggt
    10501 gtatgtctcc ggtacgctgg cttttgatca acacaataac gtgctgtttg ccgatgaccc
    10561 aaaggcgcaa acccgccacg ttctggaaac tatccgcaag gtaatcgaga cagcgggtgg
    10621 cacgatggcg gatgtgacct tcaacagcat ctttattacc gactggaaaa attacgccgc
    10681 gattaacgaa acctacgccg agttttttcc aggtgataaa ccggcgcgat tctgcattca
    10741 gtgcggactg gtaaaacctg acgcgctggt ggaaatcgcc acaattgcgc atatcgccaa
    10801 gtgaggccgc gatgaaactt tcactctcac ctccccctta tgctgatgcg cccgtagtgg
    10861 tgttgatttc gggtcttggc ggtagcggca gttactggtt accgcaactg gcggtgctgg
    10921 agcaggagta tcaggtagtc tgttacgacc agcgcggcac cagcaataat cccgacacgc
    10981 tggcagaaga ttacagtatc acccagatgg cagcggaact gcatcaggcg ctggtagccg
    11041 cagggattga gcattacgca gtggtcggcc atgcgctcgg tgcgctggtg ggaatgcagc
    11101 tggcgctgga tcatcccgcg tcggtaactg tgctggtctg cgttaacggc tggctacgaa
    11161 taaacgccca tacgcgccgc tgttttcagg ttcgcgaacg attactgtat agcggtggcg
    11221 cgcaggcatg ggtggaagcg cagccgttgt tcctctatcc cgccgactgg atggcggccc
    11281 gcgcacctcg ccttgaggca gaagacgcgc tggcactggc gcattttcag ggcaaaaata
    11341 atttactgcg tcgacttaac gccctcaaac gcgctgactt tagtcaccat gcggatcgca
    11401 tccgctgccc ggtgcaaatc atctgcgcca gtgatgatct gctggtgcca tcagcatgtt
    11461 ccagtgaact tcatgccgcc ctgcccgata gccagaaaat ggtgatgcgc tatggcggac
    11521 acgcctgcaa cgtgaccgat cccgaaacgt ttaatgctct gttactcaac gggcttgcca
    11581 gcctgttaca tcaccgtgaa gccgccctgt aaggaattgc tatgaacgaa gccgttagcc
    11641 caggtgcgct tagcaccctg ttcaccgatg cccgcactca caacggctgg cgggagacac
    11701 ccgtcagcga tgagacgtta cgggagcttt atgccctgat gaaatggggg ccgacatcag
    11761 ctaactgttc tccggcacgt atcgtgttta tccgcactgc agagggaaaa gaacgtctgc
    11821 gcccggcgct ttccagcggc aatctgcaaa aaaccctgac cgcgcccgtc accgctatcg
    11881 tcgcatggga cagtgaattt tatgaacggt taccgcaact gtttcctcac ggcgatgccc
    11941 gcagttggtt tacctccagc ccacaacttg ccgaagaaac agcgtttcgc aacagttcca
    12001 tgcaggcggc ttatctgatc atcgcctgcc gtgcgctggg gctggatacc ggcccgatgt
    12061 cgggttttga tcgtcaacac gtagacgacg ctttttttgc aggcagcacg ctgaagagca
    12121 atctgctgat taatatcggc tatggcgata gcagcaaact ttttgcgcgc ctgccacgtc
    12181 tgtcctttga agaagcctgc gggctgttgt aaggagcaaa ctatgaacat tgtcgatcaa
    12241 caaacttttc gcgatgcgat gtcctgcatg ggcgcggcgg tcaatatcat caccacggac
    12301 ggtccagccg ggcgcgccgg gttcaccgcc agcgccgtct gcagcgtgac cgacacgcct
    12361 cccacattac tggtgtgcct gaatcgtggg gcgtccgtct ggccggtatt caatgaaaat
    12421 cgaacgctgt gtgtaaatac acttagcgcc gggcaggagc cgctttcaaa cctttttggc
    12481 ggcaaaacgc ccatggaaca ccgctttgcc gccgcccgct ggcagactgg tgtgaccgga
    12541 tgcccgcaac tggaagaggc gctggtttcg tttgactgcc gtatcagcca ggtggtgagc
    12601 gtcggcaccc acgacatttt gttttgcgcc atcgaagcga ttcatcgtca cgccaccccc
    12661 tacgggctgg tgtggttcga tcgcagttat cacgcgctga tgcgccctgc ttgttaacct
    12721 ccttaaggag acagctgatg gcaatgttcg gtcttcctca ctggcagtta aaatcgacct
    12781 ctacagagag cggcgtggtc gcgccggatg aacgattgcc gtttgctcag acggcagtaa
    12841 tgggcgttca acacgcggtg gcgatgtttg gcgcgacggt gttaatgccg attctgatgg
    12901 ggctggatcc caatctttcc attttaatgt cagggatcgg cacgttactg tttttcttta
    12961 ttaccggcgg gcgcgtgccc agttatctcg gctccagcgc ggcttttgtc ggcgtggtga
    13021 tcgccgcaac gggctttaac ggtcagggca ttaacccgaa tatcagcatt gcgctgggcg
    13081 ggattatcgc ctgtgggctg gtttataccg tcattggcct ggtggtgatg aaaatcggta
    13141 cgcgctggat tgaacggcta atgccgccgg tggtgacggg tgcggtggtg atggcgattg
    13201 gcctgaactt agcgcctatc gcggtaaaaa gtgtatcggc ttcaggcttt gatagctgga
    13261 tggcggtaat gacggtgctg tgtattgggc tggtggcggt gtttacccgt ggcatgatcc
    13321 agcggttatt gattcttgta ggattaattg tcgcctgtct gctgtatggc gtgatgacca
    13381 acgttttagg gctgggaaaa gccgtcgatt ttactctcgt cagccatgct gcctggttcg
    13441 gtctgccaca tttttccact cctgctttta atggtcaggc aatgatgctg attgcgcccg
    13501 tggcggtgat tctggtggcg gaaaacttag gtcatctcaa ggcggtcgcc gggatgaccg
    13561 gacgcaatat ggacccgtat atggggcgag cattcgtagg cgatggtctg gcaacgatgc
    13621 tttctggttc tgtcggcagt agcggcgtca ccacctatgc tgaaaatatc ggtgtgatgg
    13681 ccgtgacaaa agtctactcg acgctggtgt ttgttgctgc tgccgtcatc gccatgctgt
    13741 tgggcttttc accgaaattt ggcgcgctga ttcataccat tccggccgcg gtgattggtg
    13801 gtgcatcaat tgttgtgttc gggctgattg ccgtcgcagg cgcaaggata tgggtacaaa
    13861 accgtgtcga tttaagccag aacggtaatt tgattatggt cgcagtaacc ttagtgctgg
    13921 gcgcgggtga ttttgctctc acgctgggcg gttttacgtt gggaggaatc ggtacagcaa
    13981 cctttggcgc gattttactc aatgcgttat taagcagaag gttggttgac gttccaccgc
    14041 cagaagttgt tcatcaggag ccataaacag aaaaccgccg gggttaccgg cggtgatggc
    14101 tttttgacga gcttaagaaa aaaatgcatt cgtcattgat tagcccggct agttgtcgct
    14161 tttgccgtga ctgctcttgc cacctttttt acctgcttct gatgctcttt cgcggtcttc
    14221 tgcaaaattg ccggaaccgc ctcgatggtt tgccattact gacctccgtc tgggtataga
    14281 catcatattg ctagaattaa cgcggcgtat ggcggctgcc atattttcgc tacgtgggac
    14341 taagcttagt gaactgacgt gtttctaccc gcgtttggta atatcctgca acagcttctt
    14401 agcgagaata gatatcatct ccaataatta tccataagcc gctcttatga ttaaaaaaac
    14461 gcgatgagat aaaaatgtgt cattttgcga caaaattgcg tgcttgtgaa agttgttata
    14521 aatcaaataa gtggttgtga aatttgcact ctgaaaagga cgtcttatct ttaaataagt
    14581 ggtagcgaat cgctacggaa tagagataac acgaggagtg gttagaaatg gctaaagttc
    14641 tggtgcttta ttattccatg tacggacata ttgaaacgat ggcacgcgca gtcgctgagg
    14701 gtgcaagcaa agtggatggc gctgaagttg tcgttaagcg tgtaccggaa accatgtcgc
    14761 cgcaattatt tgaaaaagca ggcggtaaaa cgcaaactgc accggttgca accccgcaag
    14821 aactggccaa ttacgacgcc attatttttg gtacacctac ccgctttggc aacatgtccg
    14881 gtcaaatgcg taccttcctc gaccagacgg gcggcctgtg ggcttccggc gcactatacg
    14941 gaaaactggc gagcgtcttt agttccaccg gtactggcgg cggtcaggaa caaaccatca
    15001 catccacctg gacgaccctt gcgcatcacg gcatggtgat tgtccccatt ggctacgcag
    15061 cgcaggaatt atttgacgtt tcacaggttc gcggcggtac gccgtacggc gcaaccacca
    15121 tcgcaggcgg tgacggttca cgccagccca gccaggaaga actgtctatt gctcgttatc
    15181 aaggggaata tgtcgcaggt ctggcagtta aacttaatgg ctaatcttca acaggaggat
    15241 acgcatgcca actcaagaag cgaaagctca tcacgtcggt gaatgggcat ctctgcgcaa
    15301 tacgtcgccg gaaatagccg aggccatttt cgaagtcgcc gggtacgacg aaaaaatggc
    15361 ggaaaagatt tgggaagaag gtagcgatga agtgttagtc aaagcctttg ctaagaccga
    15421 caaagattcg cttttttggg gcgaacagac catcgaacgt aaaaacgttt aaccagcgac
    15481 tcccccgtag cgaacggggg agttttctgt tatttcaccg cttcattcaa cacgctatca
    15541 aacttatcca tcgggcagaa accatcagcg tctatcgggc aaccgcttaa ttccagcgtc
    15601 acgcgctgcg caggtgcctg cagggttaac gcatcggcat tacgtaactg ttccgcactc
    15661 tggtacacat attcaatttt catcaaatcg cgattggctt tgctgtcacg ccaacgctgg
    15721 aaaacgattt tgccgccaat cggcgtgcgt tcgttctggt catgcaactg atacggtttg
    15781 aaatccagcg ccgttaacag agaggcaatg ttggagtcat gcccaactaa caccgtgatt
    15841 tttggagcac tggtgcgatc ggtgaccagg gctttgtcga tataactgac cagcggtttc
    15901 gcaacattgc gcgccacttc cggtgaggta aacaggctgt cctggtaacc gtttttcagc
    15961 ttcgacaaca ccttccactg ctggtcagat ttgatttctc cccaggccac ctgatccatc
    16021 ggaaaacctt cgtagtattg caaagtaaac gcatctacca gcgagttgcc gactttcagc
    16081 ggcccggaaa cacctggttc ttgttgatac ttggcgctaa aggtattttt gccatccacc
    16141 agcgaacact gttgtttctc tttacaggca ggggaatctt tatagttaac gattttttcc
    16201 agtagctggt agctgtcggt aagctggagt ttgctgagct ctttctccat tgccgccacc
    16261 gcctgttcac tgaatgcggc ggaatcatcg gtgatcaccg ggttaaaggt tgggtccata
    16321 gtgcccattt tttcctggtg atgcacagga atatcacacc ccgggaatgc gccggtaata
    16381 aagaactgtg cggtcgcaac ggtacgttgc agactattgg cataggcata aacggtgtcc
    16441 ggcggcgggc attcccccga ttttaccatc ccctgctctg ccagccattc acgcatgtaa
    16501 tggcccatat acacttcgag tacgccacct ttggtggtga gttgcccacc ggggacgtcc
    16561 cattcgggcc atttattcgg cgtcgactgc tccagcacac tgccattgtt cgccagcggc
    16621 gcacgtaagt tatggcggct catcatgagc acttgctgta gctgatagcc ttccggtacg
    16681 gtttgtgcct gagcgtttga agcgagtaaa actacccctg ccacagctgc ggcgattagc
    16741 gttttgttca ttcctggcac ctcttttgtt atcaatcact aaagagtgtg acagaaatat
    16801 gacttttttc cgggaacagg ttcgcaaaat ggcttgttga acggctgttt ttatattcat
    16861 ctttaaaaac aggcaaggtt tgacgcaaac tatgcctgat tagcccctgc ctcgcgtacg
    16921 ccgctgatga ttgcacctgt ggctaaacag aatatcggcg gcgttaccaa tacagaggca
    16981 atatttacca ataaaaaagt gtgagactaa taacaatggt ttttagcata tgcctctgtc
    17041 attaaattgc ataacgagtt aaaaaaccaa tttgcgcatt cctttcctta ttgtttaact
    17101 cgtaagcctg agcaaaaagt tcaggaggga ggtaaacgtc ataatgattg ccagtgttta
    17161 ttaaataacc atactcaaga tcttcatctg ttgaaaagga ttc
//
LOCUS       DAZKFZ010000085        17191 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000085 DAZKFZ010000000
VERSION     DAZKFZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(195..1001)
                     /gene="trpA"
                     /locus_tag="ACHOQW_002580"
     CDS             complement(195..1001)
                     /gene="trpA"
                     /locus_tag="ACHOQW_002580"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP9573643.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            complement(1001..2194)
                     /gene="trpB"
                     /locus_tag="ACHOQW_002581"
     CDS             complement(1001..2194)
                     /gene="trpB"
                     /locus_tag="ACHOQW_002581"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP9573644.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2206..3567)
                     /gene="trpCF"
                     /locus_tag="ACHOQW_002582"
     CDS             complement(2206..3567)
                     /gene="trpCF"
                     /locus_tag="ACHOQW_002582"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP9573645.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLNKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3568..5163)
                     /gene="trpD"
                     /locus_tag="ACHOQW_002583"
     CDS             complement(3568..5163)
                     /gene="trpD"
                     /locus_tag="ACHOQW_002583"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP9573646.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5163..6725)
                     /gene="trpE"
                     /locus_tag="ACHOQW_002584"
     CDS             complement(5163..6725)
                     /gene="trpE"
                     /locus_tag="ACHOQW_002584"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP9573647.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6747..6840)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6817..6861)
                     /gene="trpL"
                     /locus_tag="ACHOQW_002585"
     CDS             complement(6817..6861)
                     /gene="trpL"
                     /locus_tag="ACHOQW_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP9573648.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6999..7880
                     /gene="rnm"
                     /locus_tag="ACHOQW_002586"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6999..7880
                     /gene="rnm"
                     /locus_tag="ACHOQW_002586"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP9573649.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7877..8497
                     /gene="yciO"
                     /locus_tag="ACHOQW_002587"
     CDS             7877..8497
                     /gene="yciO"
                     /locus_tag="ACHOQW_002587"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9573650.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8525..10108
                     /locus_tag="ACHOQW_002588"
     CDS             8525..10108
                     /locus_tag="ACHOQW_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFP9573651.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            10321..11193
                     /gene="rluB"
                     /locus_tag="ACHOQW_002589"
     CDS             10321..11193
                     /gene="rluB"
                     /locus_tag="ACHOQW_002589"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP9573652.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11233..11823)
                     /gene="cobO"
                     /locus_tag="ACHOQW_002590"
     CDS             complement(11233..11823)
                     /gene="cobO"
                     /locus_tag="ACHOQW_002590"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9573653.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11820..12578)
                     /gene="yciK"
                     /locus_tag="ACHOQW_002591"
     CDS             complement(11820..12578)
                     /gene="yciK"
                     /locus_tag="ACHOQW_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP9573654.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12798..13847
                     /gene="sohB"
                     /locus_tag="ACHOQW_002592"
     CDS             12798..13847
                     /gene="sohB"
                     /locus_tag="ACHOQW_002592"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP9573655.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13883..14134)
                     /gene="yciN"
                     /locus_tag="ACHOQW_002593"
     CDS             complement(13883..14134)
                     /gene="yciN"
                     /locus_tag="ACHOQW_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP9573656.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14514..17111
                     /gene="topA"
                     /locus_tag="ACHOQW_002594"
     CDS             14514..17111
                     /gene="topA"
                     /locus_tag="ACHOQW_002594"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9573657.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         4150 a         4291 c         4595 g         4155 t
ORIGIN      
        1 tattgatttt actggtgtta tgttgcggga ttaaattgtt tgattatatt actgttgggc
       61 ggaaaaatga cgtaagttga cgttcgacag gggtaagcga aacggtaaaa agataaatat
      121 taaatgaatt taggattttt ccggcttcgt taaagaaagt taaaatgccg ccagcggaac
      181 tggcggctgt gggattaact gcgcgtcgcc gctttcatcg gttgtacaaa agctttcagt
      241 gccgccagca ttttctttgg ctcattaata tgttgctcga tgattttaac aatcgcagaa
      301 ccagaaatcg cgcccgcagc tcctgcatca atcgctgctt ttacctgatc cggggcggaa
      361 ataccaaatc cctgcaatgg aggtgccgcg ttgtactctt tcagcttcgc aaccagatga
      421 ttgaggggta acgcggcgcg gttttctgcg ccggtcacgc ccgctcgtga cagcaaatat
      481 gtgtaaccac gaccgtaaga ggctatctgg cgcagcaggt cgtcatcggc atttggcggg
      541 cagatgaaga taggtgcgac attatgacgc aacgcggcct ggcggaaggg cgcggactct
      601 tcaactggca catcggcaac cagcaccgaa tcgacgccga ctttctcgca ctgggcataa
      661 aactcatcaa tgcctttgtt aaacaccagg ttggcataca tcagcaggcc aatgggaatg
      721 gtcgggtgtt tctggcgaat gagtgccagc atctcaaagc actgcgccgg ggtcacacct
      781 gccgcaaagg cacgcagtgt ggcgttttga atcgtcgggc catctgccag tgggtcggag
      841 aaggggatgc ctaactccag cgtgtcagca ccggcttcaa ttagcgtatc gataattttc
      901 agcgactgct caatgcccgg atcaccgagc gtgacgaaag gaacgaatgc gccttctttg
      961 cgctccttca actgggcaaa cagagattcg tagcgttcca tcagatttcc cctcgtgctt
     1021 tcaaaatatc gtgaacggtg aagatgtctt tatcgccgcg accggaaagg ttaaccacca
     1081 gtagctgctc tttttccggg ttttcgcgca tcattttcag cgcatgggcc agggcgtggg
     1141 aggattccag cgccgggata atcccttcgt gcaggcacag cgttttgaag gcttccagtg
     1201 cttcatcatc ggtaattgac acataatcag cgcgtccagt gctgttaaga tacgcgtgtt
     1261 gtgggccgac ggacggaaaa tcgagtccgg cggagatgga gtaagattct tcaatctgcc
     1321 cgtcttcggt ttgcatcatc ggcgctttca taccgaaata gatacccacg cgaccatgtt
     1381 ttagcggtgc gccgtgctcg ccagtttcga taccgtgacc accaggctcc acaccaatca
     1441 ggccgacgtt ggtttcgttg atgaaatctg caaacatgcc gatggcattc gaaccgccgc
     1501 caacacaggc gataacggca tccggcaggc gaccttctct ttccagaatc tgtgctttgg
     1561 tttcttcgcc aatcatccgc tgaaactcac ggacaatggt cggataagga tgtgggccag
     1621 ctgcggtgcc cagcatatag tgcgcggttt cataactgcc ggaccagtcg cgcagcgcct
     1681 cgttacaggc atctttcagc gtcgcggaac cgctatgcac cgggatcact tccgcaccca
     1741 ttaaacgcat acgaaaaacg ttaggcgact ggcgttcaac gtctttggcc cccatataaa
     1801 tacggcattt caggccgagc agggcgctgg caagggccga cgccacgcca tgctgaccgg
     1861 ccccggtttc ggcgatgatt tcggttttac ccatccgctt cgccagtaaa gcctgaccga
     1921 gcacctggtt agttttatgc gcgccgccgt gcagcaaatc ttcgcgcttc agatacagcg
     1981 tggtgttcgt gccagccgta atgttctggc atttggtcag cgcggttgga cgcccggcat
     2041 agtttttcag caggtcgttg aactgagcct gaaattcagg atctttttgc gcgctgacaa
     2101 aagcttcttc cagctggcgc agagcaggca tcaggatttg tggcacgtac atgccgccaa
     2161 actcaccaaa ataggggtta agtaatgttg tcattgttcc tttccttaat atgcgcgcag
     2221 cgtctggaaa accgaggcca aaagacgtgc gtctttgatg cccggttgcg actctacagc
     2281 agaattaaaa tcaagtccgg cgcagccggt ttgtgccgct tccacgcagt tatctgcgcc
     2341 taagcccccc gccagcagaa cgttgccaag cgattgacca tttaatagtg accagtcgaa
     2401 acgttgcccg cttccaccct ggccgttgtc taaaacatat ttatcaacgt gctgaagctc
     2461 gcgggcgggc agggtttcac cgacgcttaa tgctttccag atggcaacgt gtgctggcag
     2521 agcttcacgc agcgtatcga tatacagctg atcttcatta ccatgcagtt gcactgccac
     2581 cagtgataac accttagctt tgttcagcac atcggcaata tcgtgattgc ggaacacgcc
     2641 aacatactgc aacggtgccg cagccatcac ttcctgcgcc tgttcaacgt tgacgcaacg
     2701 cggtgatgtc gcaacaaaaa tcaacccacc gtaaatcgcg cccgcgtcgt acgctgcttt
     2761 agcatcttgc ccacgcgtca ggccacatac tttattctca cccagcaaca cccgacgcac
     2821 ggcggcgtgc aaatcgtcat gggccatcaa cgccgaacca attagaaaac cgtcagcgaa
     2881 gtggcttaac tcgcgcacct gagcgtaagt attgatgcct gattcgctga ttaccgtcac
     2941 gttgtgcccc agtttcggcg caagctcgcg ggtacggttg agatcaatcg acaaatcacg
     3001 cagatcgcgg ttgttgatgc caacgacctt tgcccccaat gcaatggcgc gctccagttc
     3061 ctcttcatta ctgacttcgg tcagcacacc catcttcaga ctgtgggcga cggcggcaag
     3121 ctggcgatat tgttcgtcat ccagtactga aagcattaat aagcaggcat cggcctggta
     3181 atagcgcgcc agatagatct ggtaagggtc gataatgaag tctttacata aaatcggctg
     3241 cggggcgatt tggctgacga tggggaggaa atcaaagctc ccctgaaaat atttctcatc
     3301 agtcagcacc gaaattgccg aagcgtaatg tttataaatg gcggcaatgc gtgctggatc
     3361 gaaatcatca cagatcacgc cttttgacgg cgacgctttc ttgcactcca gaataaacgc
     3421 cgtgcgcgca ccctgtagcg catcataaaa atgtcgcgtg ctcggctgaa cctcattctg
     3481 aaaactggcc agcggttgct gctgtttgcg ggcttctacc caaatcgcct tgtctgcgac
     3541 gattttcgct aaaacggttt gcatcattta ccctcgtgcc gccagtgcgg tgactctgtc
     3601 gtaagcggaa ccactgcgca gtacctcaag aacggtttgc gcattggctt gcagatcttc
     3661 atggccatgc aggcgcatta acatggcgac attcgccgcg acggctgctt catgggcggc
     3721 gtcgccttta ccttgtaaca agcgtgttaa aatgtcacgg ttttcttccg gtgttccgcc
     3781 tgccagttgc tcctggtggt agggagtcag gccaaaatct tcagcggtca attgatagct
     3841 cttaatttcg ccgtcatgca gctcggcaac gattgtcggc gcgtgtaatg aaacttcatc
     3901 catcccgccg ctgtgcacca ccgccgcgcg ttgatacccc agcacgcgta aggtttcggc
     3961 aatcggcagc accagttccg gactataaac accaattaac gccagcggcg gatgcgccgg
     4021 gttaatcaat ggccccagca cattgaacag ggtgcgggtt tttagttgct ggcgaaccgg
     4081 cattgcatgg cgaaatccgg tgtgatattt cggtgcaaag aggaaacata cacctaactc
     4141 atccagcgcc tggcgcgatt tatcggcgtt catatcaaga ttaataccga acgccgccag
     4201 cagatccgac gagccagatt tactggagac gctacggttg ccgtgtttcg ccactttcag
     4261 cccacaggcc gcggcgacaa acgcactggc ggtggaaata ttgatgctgt tgctgccgtc
     4321 accgccggtg ccgacgatat cggcaaacag ataatccgga cgcgggaacg gcgcggcgtt
     4381 ttccagtagc gcggttgctg ctccggcgat ctcgttcggg tgctcgccgc gaattttcat
     4441 gctcaccagc gccgccgcca ggtgttccgg tttcagttca ccacgtacca ccgctgaaaa
     4501 tagctgatga ctttcctgct ggctaagggt ctgcgcctga tacagttttt ccagaatcgg
     4561 ttgcagcgtg ttggttggct ctagtttctg ctgcgcccag gccagcgttt gttccagcag
     4621 gcgagcgccc tgggtggtga gaatggattc cgggtggaac tggaatccgc aaacgcgatc
     4681 tgcatcgtga cgcaccgcca tcaccatgcc attaaaatgg gcgttgatgg ttaaaccggc
     4741 cggaatatta ctgccaacca gcgagtgata acgcgccacc ggcagcgggt ttgttaatcc
     4801 ggcaaacatc gcctgaccgt catgttcaat gctcgacgct ttaccgtgaa gaatttcgcc
     4861 cgcctgaccg acatagcccc cgtaagcttc gacaatcgcc tgatgaccaa ggcaaatgcc
     4921 aataataggc agcttaccgc gcaggcgggt aagcagttca ggcatacaac cagcttcgct
     4981 cggcacaccg ggtccaggag aaagcatcag caccggattg ctcatcgtcg caaggcgttc
     5041 aattaaggtc tgcgccggaa tatggttgcg gtaaatcacc acgttatgac cattgctgcg
     5101 caactgatct gccaggttgt aagtaaaaga gtcgatatta tcgagcagca gaatgtcagc
     5161 cattagaacg tctcctgtgc atgatgcgcg gtggcaatag cgcgcagtac agcgcgggct
     5221 ttattacggg tttcgtcggc ttccgactgc ggaacagaat caaggactac gccagcaccg
     5281 gcttgcacgg tagcgatacc gttttccacc agcgccgagc ggatcacaat gcaggtgtcg
     5341 agatcgccgt gcgcggtgaa ataacctacc gcgccgccgt agctgccgcg gcgacgacct
     5401 tcagcctcgg caattaactg catagcgcgt actttcggcg caccgcttaa cgtccccata
     5461 ttcatacagg cgcggtaagc gtgcagggcg tcgagatcgt ggcgcagctc accgaccacg
     5521 cgggagacca ggtgcatcac gtaagagtaa cggtcaactt tggtgaggtc ggcgacgtag
     5581 cggctgccgg gggtgcaaat gcgtgccagg tcattacggg cgagatccac caacatcaga
     5641 tgttcagaaa gctctttatg atcggtacgc atttccagtt caatacggct ttcaaggtcc
     5701 ctgtcgagcg agccatcggc gcgacgaccg cgtggacgtg ttccggcaat cgggtagatc
     5761 tcaatctggc ggctggtggc gtcatacttg agcgaacttt ccggcgacgc gccaaacagg
     5821 gtgaaatcat tatcctgcat aaaaaacatg tacgggctgg gattactctt tttcagcacg
     5881 taataggccg ccagcggtga cgggcagggc agagagaaac ggcgagatgg caccacctgg
     5941 aaaatttctc cggcgcgaat cgctttttgc aacaaacgca ctacgccacc gaactcttca
     6001 tcgctctggt tacattcaca acgcatatgc ggcacggaaa ccaccggcag cggtggcgcg
     6061 gtttcggtca gttgctggcg aagatcgttc aggcgagcag tgagacgttg tttttcttct
     6121 tcattcggag caaacaggct ggcctgaatg cgggtgcttt ttttctgatg gtcaatcacc
     6181 atcagagttt cagcgagata aaaacagaaa tcagggcagt tattttccgc tgacagttgc
     6241 ggtaaatctt caaaccccgc cacaaggtca taagagaaca ggccgccgaa gaacatggct
     6301 tctcgttctt ccttcggtac attcaacaga ttctgcaata agcggaaagc gtcaaaaacc
     6361 gaaagggagc ataagcgagc gtcttcatcc agcagtggac taacaggggg gaagcgcagc
     6421 acacggcagt ttggtaattg ttcattttcc acacccgcag gcagggcgtt atccagtagt
     6481 gtcagcaggg cttcgccgtt gccggaaagg gactggattg tgacagtgtc acctaaagct
     6541 gtaatgcgca gcgcactgtc taccagcagc aggcttttta aatcatcttt gctgtcgata
     6601 tctgcggatt ccagcagcag cgttgccgga cgatccccac acaattggtg aaaaagcgcg
     6661 gtgggattgt cgcgataagc gccttcgcag gttagcagtt cgagagtcgg tttttgtgtt
     6721 tgcattgtta ttctctaatt ttgttcaaaa aaaagcccgc tcattaggcg ggctgggtat
     6781 ctgattgctt tacgcatggt gaatacactg cccgtttcag gaagtgcgcc accaaccttt
     6841 cagtacgaaa attgctttca ttgtcgatac cctttttacg tgaacttgcg tactagttaa
     6901 ctagttcgat gattgattgt caacagctca tttcagaata tttgccagaa ccgttatgat
     6961 gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg aattacgcag
     7021 tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg ccagaagcat
     7081 tggtgcaccg tgcagtcgag atgcgcgtcg gcaccctggc gatcaccgac catgacacca
     7141 cagctgcaat cgcgccagca agagaggaaa tttcacgttc gggactggcg ctgaatctta
     7201 ttcccggcgt ggaaatttcc acggtctggg aaaatcatga aattcatatt gtcgggctga
     7261 atattgatat tactcatccg ctaatgtgtg agttcctcgc gcagcagaca gaacggcgca
     7321 atcagcgggc gcagcaaatt gccgaaagac ttgaaaaagc gcatatacct ggggcgctgg
     7381 aaggcgcaca acgactggcg cagggcggcg cagtgacgcg cggtcatttt gcgcgtttcc
     7441 ttgttgagtg cggcaaagcc agttcaatgg cggatgtctt taaaaagtat ctggcgcgcg
     7501 ggaaaaccgg atacgttccg ccacagtggt gtacaataga acaagctatt gatgtcattc
     7561 atcattctgg cggtaaggcg gtgctagctc atcctgggcg gtacaatctt agtgctaaat
     7621 ggctgaaaag attggttgcg cattttgccg aacacgacgg cgacgcgatg gaagtcgcgc
     7681 agtgccagca atcgcccaat gaacgtaccc agctggcgac gcttgcccgc cagcaccatt
     7741 tatgggcatc acaaggatct gattttcatc agccatgccc gtggatcgaa ctgggtcgta
     7801 aactctggct gcccgcaggc gttgaaggcg tctggcagct atgggaacag ccgcagaaca
     7861 ccacagagag ggaattatga gccagttttt ttatattcat cctgataacc cgcagcaacg
     7921 tctaatcaac caggcggtgg agatcgtgcg taaaggcggg gtgattgttt atccaactga
     7981 ttccggctat gcgctcggct gtaaaattga agataaaaac gccatggagc gtatttgtcg
     8041 tattcgccag ctgccggacg gtcacaactt taccctgatg tgtcgcgatc tttctgaact
     8101 ctcgacctat tcatttgtcg ataacgttgc gtttcgttta atgaaaaaca acacgccggg
     8161 caactatacc ttcatcctga aggggacgaa ggaggtgcca cgccgtctgt tgcaggaaaa
     8221 acgcaaaacc atcggtatgc gtgtgccttc taacccgatt gcgcaggcgt tgcttgaagc
     8281 actgggcgaa ccgatgcttt ccacttcgct aatgctgcca ggcagcgaat ttaccgaatc
     8341 cgatcccgaa gaaatcaaag accgtctgga aaaacaggtc gatttgatta ttcacggcgg
     8401 ctatctcggg cagaaaccaa caacggttat tgatcttacc gatgatacgc cggtcgtggt
     8461 gcgtgaaggc gtaggtgatg tgaagccttt cttataacaa attgcgttcc acggatggaa
     8521 gactatggca gggaagtttc gctgcatttt gctgttgata gttgggcttt ttttctcttc
     8581 gttaagttat gcgaaaaaca cggagatgcc ttcttatgaa gaagggatct cgctctttga
     8641 tgttgaagcc actctgcaac cggatggggt gctcgacatc aaagaaaata ttcattttca
     8701 ggcgcgaaat cagcagatta agcacggatt ttatcgtgat ttaccacgac tatggatgca
     8761 gcctgatggg gacgctgcac tgctgaacta tcatattgtt ggcgtcaccc gtgatggtat
     8821 tcctgaaccc tggcatcttg actggcatat cgggttaatg agtattgtcg tgggcgataa
     8881 acaacgtttc ttgcctcaag gcgactatca ttatcaaatt cattatcagg ttaaaaatgc
     8941 tttcctgcgt gagggagatt cagatctgtt aatctggaac gtgactggta accactggcc
     9001 gtttgaaatc tataagaccc gattttcact caagttccct gatatcgcgg gtaatccatt
     9061 tagcgaaatc gatctcttta ctggagaaga gggcgacaca tatcgaaatg gccgcatcct
     9121 tgaggacgga agaattgaat ccagcgatcc gttttatcgt gaagatttca cggtactcta
     9181 ccgttggcct cactcgttac ttagcaatgc cccggctcca caaacgacga atattttcag
     9241 ccatcttctt ttaccctcca cgtcatcgtt gttaatttgg tttccgtgtc tattcctggt
     9301 ttgtggatgg ttatatctct ggaagcgcag gccgcaattt acgtcggtag atgttttacg
     9361 gatgcagaaa ttttatctgc cgcgtaaaaa gtcttcgttt taccggcctg atactttttt
     9421 gcaatggggt ggactggcaa tattggcggt cattctttac ggtaacctga gtcctgtagg
     9481 ctgggcagga atgagtctgg tgggcgatat gtttattatg atctgctggc ttcttccttt
     9541 tttattttgt tcccttgagc ttttgtttgc ccgcgatgat gacaagcctt gcgttaatcg
     9601 tgtaatcatc actttgtttt taccgctgat ttgttcaggc gtggcctttt attctctcta
     9661 tatcaatgtc ggagatgtat tcttttactg gtatatgcca gcgggttatt ttagcgctgt
     9721 tttcctgacc ggttatctca ctggcatggg gtatattttt ctgccaaagt ttacccaaac
     9781 tgggcagcaa cgttatgccc acggtgaagc tatcgttaac tatctcgcgc gtaaagaggc
     9841 agcaacacac agtgggcgtc ggcggaaagg ggaaacacgg aaactggatt acgcgttgct
     9901 aggttgggct gtctcagcaa accttggaag agaatgggta gcacgtatca ccccatcact
     9961 cacagcggct gttcgcgccc cggaaattgc ccgtagtggc gttttgttct cattacagat
    10021 gcacctgagt ctgggggcca ataccagttt attggggcga agttattccg gtggtgctgc
    10081 gggtggcgga ggcggtggtg gctggtaatt aagctgatgt taatcgaaac aagctttaac
    10141 cataaagata gtgtagtatg ttgcgcctca aagccaggcc agttaaaacg tcgagtcgtt
    10201 tacttaaggc ctgaagagtt caaacagact ttactatcag tgagtcagat gtaagttctg
    10261 actcactgtt gcacaaaagc atatcagacg cctgggaagg cgacaccaaa aggaagctct
    10321 atgagcgaaa agctacagaa agtgctggcg cgtgccggcc acggttctcg ccgtgaaatc
    10381 gaatctatta ttgaagccgg tcgtgtgagt gttgatggca aaattgctaa actcggcgat
    10441 cgcgttgaag ttacccctgg attgaaaatc cgtatcgatg gtcacctgat ttcggtacgt
    10501 gagtccgctg aacaaatttg tcgcgttctg gcctattata aaccggaagg tgagttgtgt
    10561 acccgtaacg atccggaagg gcgtccaacg gtgtttgacc gtctgccaaa gctgcgcggt
    10621 gcacgctgga ttgccgtagg tcgtctggac gttaacacct gtggtctgtt gctgttcacc
    10681 accgacggtg aactggctaa ccgtttaatg catccaagcc gtgaagttga acgtgaatat
    10741 gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc gtgatttaag ccgtggtgtg
    10801 cagctggaag atggtccggc agcctttaaa accatcaagt tcagcggcgg cgaagggatc
    10861 aaccagtggt acaacgtgac tctgaccgaa ggccgtaacc gtgaagttcg tcgtctgtgg
    10921 gaagcggttg gcgtgcaggt aagccgcctg atccgtgttc gttacggtga tatcccactg
    10981 ccgaaaggtc tgccacgcgg cggctggacg gagctggatc ttgcccagac taactatctg
    11041 cgcgaactgg tggagttgcc gccagaaacc agctctaaag tcgctgtaga aaaagaccgt
    11101 cgtcgcatga aggcgaatca gattcgtcgt gcggtgaaac gtcacagtca ggtgagcggc
    11161 ggacgtcgtt ctggtgggcg taataacggt taatcaacgt atgccggacg tcatatccgg
    11221 catttttaca gattagtaat caatccctat ctgcgctttt acgccagcat caaacgcatg
    11281 tttcacgggg cgtaactcac ttaccgtatc cgccagttcg agtatatccc gatgacagcc
    11341 ccgacccgtg ataatcaccg tctgctgatg tggacgttca tttaacgcct gcaacacttc
    11401 ttccagtggc aaatagtcat acgccaccat ataagtcagc tcatcaagca aaaccaaatc
    11461 cagtgaggaa tcagcaagca tccgctttgc atgttgccag acttcgcggc aggcggcggt
    11521 atcagactcg cggttttgtg tatcccaggt aaagcccgtt gccatcacct gaaactcaac
    11581 gccatgtggc tccagcagat tgcgttcgcc attaggccag gtgcctttaa taaactgcac
    11641 gacgcccact ttttttccgt gaccaactgc gcgtgttgcc gtaccaaatg ccgcggtggt
    11701 tttgcctttt ccattgccgg taaagacgat gataataccg cgttcatcct gggcctgggc
    11761 catacgagca tctacttttt ctttcactcg ctgctgacgc tgttggtagc gttcatcact
    11821 cattgggaaa ttcctggttt acggcccggt tgggcgtcaa aggtcatgcc ggttttacgg
    11881 cggctgtcat cgcccatcag ccagaggtag agcggcatga tatcagcggg tgttttaagt
    11941 ttctgtggat cttcggtcgg gaaggcgctg gcacgcattg cggtgcgcgt accgcctggg
    12001 ttaatgcagt tgacacgcag gcgctgctga tattcatcgg ccagtacctg catcatccct
    12061 tcggtggcaa atttcgacgc tgcatatgca ccccagttgg ctcgtccctg acgtccaacg
    12121 cttgatgaag taaagaccag tgaaccggcg tccgatttga gtaataaagg aagtagtgcc
    12181 tgggtgagca taaaggtggc attaacgttg acctgtatga cgtcctgcca gacctgtgga
    12241 ttttgttcgc tcattggaca aacatcgccg agtaatccgg cattatgcaa aacaccatcc
    12301 agacgcggat aattaacggc aatgcgctgt gccagttgtt ggcaatcttc ggacctgcag
    12361 gtcagcaaat cgagaataaa ccactgtggc tgacgcccag tttcttcgtt tatgtggctg
    12421 gctacctgac gtaatttttc ttcattacgg cccaacagaa tcactgtcgc accatagcgt
    12481 gcatacgtca tcgcggcttc acgaccaata ccatcactgg ctcccgtcac caggataatg
    12541 cgatcattga gtaaatcttg ttttggctgg taatgcatgg ctactcctca acgacgttgt
    12601 ctgtcgcgct gccagtgtac tttatgactt tggggcttta tgcctgaaac agaagggcat
    12661 ttcaatcagc ctcgggcgat aacaccgcaa aatggatact ttgtcatact ttcgctgcaa
    12721 taacatctct gcgagacggc ttaacatgcc tgttgtaaac tgtgagccaa agcgttgttt
    12781 aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg tttgtttttg gcgaaaatcg
    12841 ttaccgttgt gctagcgatt gcggcgattg ccgccattat tgtcaatgtt gctcaacgta
    12901 ataaacgcca gcgtggcgag ttacgggtca acaatctcag cgaacagtat aaggagatga
    12961 aagaagaact ggccgcggcg ctgatggact cacatcagca aaaacagtgg cacaaagcgc
    13021 agaagaaaaa gcacaagcaa gaagcgaaag cagcaaaagc gaaagccaaa ctgggcgagg
    13081 tgtcaactga cagtaaaccc cgcgtctggg tgctggactt taaaggcagc atggacgccc
    13141 atgaagtgaa ctcgctacgt gaagagataa cggcggtact tgcagcattc aaaccgcagg
    13201 atcaggttgt gctccgtctg gaaagccctg gtggcatggt gcatggttac gggttggcgg
    13261 cttcgcagct gcagcgtctg cgtgataaaa acattccatt aactgttacg gtagacaaag
    13321 ttgctgccag cggtggttac atgatggcct gtgtggcgga caaaattgtt tccgcaccgt
    13381 ttgctattgt gggttccatt ggggtggtgg cgcaaatgcc caactttaac cgcttcctga
    13441 aaagcaaaga tattgatatc gaactgcaca ccgccgggca gtataagcgt acgctgacct
    13501 tgctgggtga aaataccgaa gaagggcggg agaaattccg cgaagagctg aacgaaacgc
    13561 atcagttatt taaagatttt gtgaagcgta tgcgtccgtc tctggatatt gaacaggtgg
    13621 caacgggtga acactggtac ggacaacagg cggtagagaa gggcctggtt gatgaaatca
    13681 acaccagtga tgaagttatt cttagcctga tggaaggccg agaagtggtc aatgtacgct
    13741 atatgcagcg taaacgactc attgaccgat tcaccggcag cgcggcagag agcgccgatc
    13801 gattattgct acgctggtgg cagcgtgggc aaaagccatt gatgtaaaag acaaacgcga
    13861 gggtaagacc tcgcgttttg ctttaatcaa ccagatgata tttttctgaa agcacatggg
    13921 ccaggtgttt gaacatatta aacaccgcgg tgcttttggc tgttggcaat ccttgttcat
    13981 ctaaaaagta gtcgccggta aataccagca cgccattacg ctgtgtgacg ccggtggctt
    14041 caatccctgc gagcgtatcc tcatgctcac gaatgatttt gttggcctct ttcaacagcg
    14101 tttcgcggtc gatgggttgt gtctctttgt tcattattta ctccttaaac aaggacatta
    14161 gtctacgcca ggcatggctt gcagacaaat ataccacgct ggtggcaaga gcgccttact
    14221 ggcaactttg gattttgcat gctaataaag ttgcgtatcg gattttatca ggtacagtgt
    14281 gacgctttcg tcaatctggc aatagatttg cttgacattc gaccaaaatt tcgtcgtgct
    14341 atagcgcctg taggccaaga cctgttaact cagtcacctg aattttcgtg aacagagtca
    14401 cgacaagggg ttgatatccg cagagagcga gtccatatca gtaactcgtt gccagtggaa
    14461 ggtttatcaa cgtgcgacgc attcctggaa gaatcaaatt aggtaaggtg aatatgggta
    14521 aagctcttgt catcgttgag tccccggcaa aagccaaaac gatcaacaag tatctgggta
    14581 gtgactacgt ggtgaaatcc agcgtcggtc acatccgcga tttgccgacc agtggctcag
    14641 ctgccaaaaa gagtgccgac tctacctcca ccaagacggc taaaaagcct aaaaaggatg
    14701 aacgtggcgc tctcgtcaac cgtatggggg ttgacccgtg gcacaattgg gaggcgcact
    14761 atgaagtgtt gcctggtaaa gagaaggtcg tctctgaact gaaacaactg gctgaaaaag
    14821 ccgaccacat ctatctcgca accgaccttg accgcgaagg ggaagccatt gcatggcacc
    14881 tgcgggaagt gattggtggt gatgatgcgc gctatagccg agtggtgttt aacgaaatta
    14941 ctaaaaacgc gatccgccag gcatttaaca aaccgggtga gctgaatatt gatcgtgtta
    15001 atgcccagca ggcgcgtcgc tttatggacc gcgtggtggg gtatatggtt tcgccgctgc
    15061 tatggaaaaa gatcgctcgt ggtctgtctg ccggtcgtgt gcagtcggtg gcagtccgcc
    15121 tggtggtcga gcgtgagcgt gaaattaaag cgttcgtgcc ggaagagttc tgggaagtcg
    15181 atgccagcac gaccacgcca tctggtgaag cgttggcgtt gcaggtgact catcagaacg
    15241 acaaaccgtt ccgtccggtc aacaaagaac aaactcaggc tgcggtaagt ctgctggaaa
    15301 aagcgcgcta cagcgtgctg gaacgtgaag acaaaccgac aaccagtaaa cctggcgctc
    15361 cttttattac ctctacgctg caacaagctg ccagcacccg tcttggattt ggcgtgaaaa
    15421 aaaccatgat gatggcgcag cgtttgtatg aagcaggcta tatcacttac atgcgtaccg
    15481 actccactaa cctgagtcag gacgcggtaa atatggttcg cggttatatc agcgataatt
    15541 ttggtaagaa atatctgccg gaaagtccga atcagtacgc cagcaaagaa aactcacagg
    15601 aagcgcacga agcgattcgt ccttctgacg tcaatgtgat ggcggaatcg ctgaaggata
    15661 tggaagcaga tgcgcagaaa ctgtaccagt taatctggcg tcagttcgtt gcctgccaga
    15721 tgaccccagc gaaatatgac tccacgacgc tgaccgttgg tgcgggcgat ttccgcctga
    15781 aagcacgcgg tcgtattttg cgctttgatg gctggacgaa agtgatgcct gcactgcgta
    15841 aaggcgatga agatcgtatc ttacctgcag tcgataaagg cgatgctctg acgctcgttg
    15901 aactgacacc agcccagcac tttaccaagc cgccagcccg tttcagtgaa gcatcgctgg
    15961 ttaaagaact ggaaaaacgt ggtatcggtc gtccgtctac ctatgcgtcg atcatttcga
    16021 ccattcagga tcgtggctat gtgcgagtag aaaatcgtcg tttctatgcg gaaaaaatgg
    16081 gcgaaatcgt caccgatcgc ctggaagaga atttccgcga gttaatgaac tacgacttca
    16141 ccgcgcagat ggaaaacagc cttgaccagg tggcaaatca cgaagcagag tggaaagctg
    16201 tactggatca cttcttctcg gatttcactc agcagttaga taaagctgaa aaagatccgg
    16261 aagagggggg tatgcgtccg aaccagatgg ttctgaccag catcgactgc ccgacctgtg
    16321 gtcgcaaaat ggggattcgc acagcgagca ccggggtatt ccttggctgt tctggctatg
    16381 cgctgccgcc gaaagagcgt tgcaaaacaa ccattaacct ggtgccggaa aacgaagtgc
    16441 tgaacgtgct ggaaggcgaa gacgctgaaa ccaacgcgct gcgcgcaaaa cgtcgttgcc
    16501 ccaaatgcgg cacggcgatg gacagctatc tcatcgatcc gaaacgtaag ttgcatgtct
    16561 gtggtaataa cccaacctgc gacggttacg agatcgaaga gggcgaattc cgcattaaag
    16621 gttatgacgg cccgatcgtt gagtgtgaaa aatgtggttc tgaaatgcac ctgaaaatgg
    16681 ggcgtttcgg taaatatatg gcctgcacca acgaagagtg taaaaacacg cgtaagattt
    16741 tacgtaacgg cgaagtggct ccaccgaaag aagatccggt accattaccg gagctgccgt
    16801 gcgaaaaatc agatgcctat ttcgtgctgc gtgacggtgc tgccggtgtg ttcctggcgg
    16861 ccaatacctt cccgaaatcg cgtgaaacgc gtgcgccgct ggtggaagag ctgtatcgct
    16921 tccgcgatcg tctgccggaa aaactgcgtt atctggccga tgcgccgcag caggatccgg
    16981 aaggtaataa gactatggtt cgctttagcc gtaaaaccaa acagcaatat gtctcttcgg
    17041 aaaaagacgg aaaggcgact ggctggtcag cattttatgt tgatggcaaa tgggttgaag
    17101 ggaaaaaata acctttaatt ctgtcaggtt tttataaaca aagggtcgcg aaagcggccc
    17161 ttttttattg catattattt tttcttcaca c
//
LOCUS       DAZKFZ010000086        17181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000086 DAZKFZ010000000
VERSION     DAZKFZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHOQW_002595"
     CDS             complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHOQW_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP9573658.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1564..2769)
                     /gene="hemX"
                     /locus_tag="ACHOQW_002596"
     CDS             complement(1564..2769)
                     /gene="hemX"
                     /locus_tag="ACHOQW_002596"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP9573659.1"
                     /translation="MTEQEKTSAVVEETKEAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     TPAAAPQGE"
     gene            complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHOQW_002597"
     CDS             complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHOQW_002597"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP9573660.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHOQW_002598"
     CDS             complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHOQW_002598"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP9573661.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHOQW_002599"
     CDS             4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHOQW_002599"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP9573662.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHOQW_002600"
     CDS             complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHOQW_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP9573663.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(7924..8066)
                     /locus_tag="ACHOQW_002601"
                     /pseudo
     CDS             complement(7924..8066)
                     /locus_tag="ACHOQW_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8225..8428
                     /gene="yifL"
                     /locus_tag="ACHOQW_002602"
     CDS             8225..8428
                     /gene="yifL"
                     /locus_tag="ACHOQW_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9573664.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8465..9289
                     /gene="dapF"
                     /locus_tag="ACHOQW_002603"
     CDS             8465..9289
                     /gene="dapF"
                     /locus_tag="ACHOQW_002603"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9573665.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9286..9993
                     /gene="yigA"
                     /locus_tag="ACHOQW_002604"
     CDS             9286..9993
                     /gene="yigA"
                     /locus_tag="ACHOQW_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFP9573666.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            9990..10886
                     /gene="xerC"
                     /locus_tag="ACHOQW_002605"
     CDS             9990..10886
                     /gene="xerC"
                     /locus_tag="ACHOQW_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9573667.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10886..11602
                     /gene="yigB"
                     /locus_tag="ACHOQW_002606"
     CDS             10886..11602
                     /gene="yigB"
                     /locus_tag="ACHOQW_002606"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP9573668.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11686..13848
                     /gene="uvrD"
                     /locus_tag="ACHOQW_002607"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11686..13848
                     /gene="uvrD"
                     /locus_tag="ACHOQW_002607"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP9573669.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13893..14795)
                     /locus_tag="ACHOQW_002608"
     CDS             complement(13893..14795)
                     /locus_tag="ACHOQW_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFP9573670.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14878..15642)
                     /gene="yigE"
                     /locus_tag="ACHOQW_002609"
     CDS             complement(14878..15642)
                     /gene="yigE"
                     /locus_tag="ACHOQW_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP9573671.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15804..15860
                     /gene="ysgD"
                     /locus_tag="ACHOQW_002610"
     CDS             15804..15860
                     /gene="ysgD"
                     /locus_tag="ACHOQW_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP9573672.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16012..16962
                     /gene="corA"
                     /locus_tag="ACHOQW_002611"
     CDS             16012..16962
                     /gene="corA"
                     /locus_tag="ACHOQW_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP9573673.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17004..>17181)
                     /locus_tag="ACHOQW_002612"
     CDS             complement(17004..>17181)
                     /locus_tag="ACHOQW_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573674.1"
                     /translation="PTIKSNDNCITKNYLMVINEYPYRNTHIGINDFTEYQLTQENKI
                     EQVFNPHNFKKDNF"
BASE COUNT         3913 a         4487 c         4579 g         4202 t
ORIGIN      
        1 aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa ttatctcata
       61 acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacga gtagatgctc
      121 attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt
      181 acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc atggacacaa
      241 cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata
      301 agagcaggcg tttttttatg tgtcaggaag gtcccggagg tgcttgcctc cgggtgagaa
      361 ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga cgcatcgctg
      421 cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg
      481 cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc cattctccgt
      541 gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg acgtttttga
      601 tttgctggcg cagcactttt tccagctgtt ccggattgtt tgttttcagt cgaggaatcg
      661 gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt tgctgggcag
      721 tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac gctacctgat
      781 gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcgcta ccgttatcgg
      841 cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca cgatgttctt
      901 catcaccaac atgggctttc gccattgatg ggataatatc cagcagcgaa ctccatgcac
      961 ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt ggcgtaactt
     1021 ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc agttgcagac
     1081 gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct gcgcgttcca
     1141 gatgttggtt ggcgcgcgct tcatcaccac gttgttgcgc ggcttcggca gccagtagat
     1201 agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt tcaacttgct
     1261 gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt gcacggcgac
     1321 gcttacgccc gacaaaccac ccacgggtgt gcgcgccagt gcggaagatc cgccgcagta
     1381 gccactcaat ggcaaacagc actaccatcg ccagaatcaa tatgatcgcc aggcccgtga
     1441 cgctggtttc gatattgtag ttgtcggtct ggatcagcac ataaccctga tgaccggcaa
     1501 tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac acttttagca
     1561 tggttattct ccttgcggcg cggctgccgg agtatcagct tgcggtgcag gtgcaggtgc
     1621 aggtgcaggt gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt gtgccagcag
     1681 gttacgcacg cgagtctgca tcagtttttc cagcatcgcc tggctttgca gggtttccgg
     1741 aagatccatc gagatatttt gctggcttaa ctggtctacc tcgtcgagga acgctttggt
     1801 ggtggcatca tcagtatcgt agtaagcacg tacccaggtg gagacgttct ccagcgcctg
     1861 gcgataagtc tcttgctggt gacgcggtac agcttgtgct gcgaccagca ggcgagagcg
     1921 aatattttcg cgcagataga tatcctgatt tggcgctaac agcggtacgg cggtgtcatc
     1981 acgacggcga atcgtaatga agttgtccat aaagttctgc cagctttttt gcagattgat
     2041 acgccattcg ctgatggaac tggaaagctc ttcaccgtcg gaatccatcg gcgaaccatc
     2101 gctgtcatta tcggccagac gcaggttatc tacctgattt gaaagctgat taagcttaag
     2161 gatgatgccg tcataatcca cctgcgatac tgcagaaagg ctggcgatat catcggtaat
     2221 tgcccgacga acggtaatca gactcgggtc attcatatcc gccaggctgg cgtctgcact
     2281 tttcagcaac gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc gtccggcgag
     2341 tttcaccaga aaatcggcct gagccagcag ccaggtttta gcatcgctgc cggaaatggt
     2401 ggcgaccttt tgttggactt catccaactg ttttgccagc gtttcttgct gacgattcgc
     2461 ctgcttaagc tgtgcagctt gttgcttaat aatgccttcc agctcgactt tttggctctc
     2521 ctgggctttt tgcaatgccg tcagttggtt agccagggca tcgctggtgg cggtctgatt
     2581 gacggcctgt tgtttacccc agccatacaa accgacgccc gccgccagag caatagcgat
     2641 agccaccgcg ctgagaatca atgcggtatt gttcttactc tttttttctg ttgcgacagg
     2701 ttgtgacgtg gtgtccacgg cctccttggt ctcttcaacc acggcggagg ttttttcttg
     2761 ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt
     2821 gtcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga gtttcgccaa
     2881 acgctcactg acgaccaata gtcgacagtg tagtaaccag tgctcacgat accattgtgg
     2941 gatcagcgac cagagttgct gcaacatttc accgctggta acaacgaccg tcgtcacctc
     3001 gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattgcgc atcgttgata
     3061 acattcacaa aaagtgacct cagcaccgcg cgccgtcagg gtatccccaa ttagctcacg
     3121 accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta attcaggtaa
     3181 ttgtagcaag acttcgctga tttcccgatc ctgcgggtag agaatcttct gtccgcttac
     3241 ggtatgtagt gccagtgcgg tggtgcgtcc aatggcgaaa taattaggta gtcggggcca
     3301 tttacgatct tgctgatgta gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc
     3361 aaacaacaga tcgctctccc ccagcgctgc cagttgatca gcaagttgcg gtaattgtcg
     3421 acccggagaa aactcaatca gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg
     3481 gctcactaac tcttctccag cgggagacgg gcgggtgaca aggatactca tgccggggcg
     3541 tctccgttat agacttcagc gaggatctcg cgcgcgccgt tattcagtag ctcttctgcc
     3601 agcgaaatcc ccatttgttc ggcatcttgc ggcgcaccgc ggcgttcacc gcgaataatc
     3661 tgcgaaccgt ccggcgcgcc gaccagcgca cgcagccaga tttcgccatc aataagctcg
     3721 gcgtagctac caattggcac ctgacatcca ccttcgagac gggtattcat ggcgcgttct
     3781 gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc gcgagtgcgt
     3841 gtatcatcaa ggcggcattc aataccgact gcaccttgtc ctaccgccgg aagagaaatc
     3901 tcgggtggca acgcggcgcg aatgcgggac tccagaccta aacgttttag tccggctacg
     3961 gcaagaatga tggcatcgta ttcgccgtta tccagtttgc tcaggcgagt gccgacgttg
     4021 ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca ctggcgacgt
     4081 aaactggacg tcccgacgat actgcctgcc ggtaacgcat ccagactgtc atagttattg
     4141 gacacaaagg catcgcgagg atcttcacgc tcacaaatag tgaccaggcc cagaccttgc
     4201 gggaattcaa ccggcacatc tttcattgaa tgtacggcga tatcggcgcg attttcgagg
     4261 agcgcgactt ccagctcttt tacaaataag ccttttccgc cgactttcgc cagcggcgta
     4321 tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caacgaccag gcccggatgg
     4381 ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt
     4441 gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat ccgtggtcca
     4501 tcctaacatc cttgccagag tgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa
     4561 ccgtaaaatg aggtctggca gtggatcctg acaggcgttt cacgccgtcg taataaggaa
     4621 tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta cggtcaatca
     4681 gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact aaaaaaacca
     4741 ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg ccgcgtgtta
     4801 ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat acgtcttgta
     4861 cctctatatt gagactctga aacagagact ggatgccata aatcaattgc gtgtggatcg
     4921 cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc cgacattgtt
     4981 gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca aaggcatttg
     5041 cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg
     5101 aatgtcagta caggacccgc cgaaaggtga gcttccaatt actggtgtat acaccatggg
     5161 cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg tctgtcatca
     5221 atcctggctc gatagcgaag agcgccagtt gctacaacgt aaatgcagcc tgctggaaag
     5281 ctgggccgcc tcgctgggtg tggaagttag cttcttcctg attgatgaaa accgttttcg
     5341 ccacaacgag agtggcagtc tggggggcga agattgtggc tccacccagc atatactgct
     5401 gcttgatgaa ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc tgtggaatat
     5461 ggtgccgtgc gacgaagaag agcattacga tgactacgta atgacgctct acgcacaagg
     5521 cgtgctgacg ccaaatgaat ggctggatct cggcggctta agctcgcttt ccgctgaaga
     5581 atacttcggt gccagccttt ggcaactcta caagagtatc gattctccat acaaagcggt
     5641 gctgaagaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac gcctgctggc
     5701 gaaagatatc aaacagcgtt tgcacgacgg cgagattgta tcgtttggcc tcgatcctta
     5761 ctgcatgatg ctggagcgtg ttactgaata cctgacggcg attgaagatt tcacccgtct
     5821 ggatttagta cgtcgctgct tctatttaaa agtgtgcgaa aagctcagcc gtgaacgcgc
     5881 ctgcgtaggc tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg ggtgggacga
     5941 agctcgtctg gcaatgctcg ataaccgcgc taactggaag attgatcagg tgcgtgaggc
     6001 gcacaacgag ttgctcgacg cgatgatgca gagctaccgt aatctgatcc gctttgcgcg
     6061 tcgcaataac cttagcgtct ccgccagtcc gcaggatatc ggcgtgctga cgcgtaagct
     6121 gtatgccgcg tttgaagcat taccaggtaa agtgacgctg gtaaacccgc agatttcacc
     6181 cgatctctcg gaaccgaatc tgacctttat ttatgtgccg ccgggccggg ctaaccgttc
     6241 aggttggtat ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc atcagccgct
     6301 ggaatataac cgttacctga ataaactggt ggcgtgggca tggtttaacg gcctgctgac
     6361 ctcgcgcacc cgcttgtata ttaaaggtaa cggcattgtc gatttgccta agttgcagga
     6421 gatggtcgcc gacgtgtcgc accatttccc gttgcgctta cctgcaccga caccgaaggc
     6481 gctctacagc ccgtgtgaga tccgccatct ggcgattatc gttaacctgg aatatgaccc
     6541 gacagctgcg ttccgcaatc aggtggtgca ttttgatttc cgtaagctgg atgtcttcag
     6601 ctttggcgag aatcaaaatt gcctggtagg tagcgttgac ctgctgtatc gcaactcgtg
     6661 gaatgaagtg cgtacgctgc acttcaacgg cgagcaatcg atgatcgaag ccctgaaaac
     6721 tattctcggc aaaatgcatc aggacgccgc accgccagat agcgtggaag tcttctgtta
     6781 tagccagcat ctgcgcggct taattcgtac tcgcgtgcag caactggttt ctgagtgtat
     6841 tgaattgcgt ctttccagca cccgccagga aaccgggcgt ttcaaggcgc tgcgcgtttc
     6901 tggtcaaacc tggggcttgt tcttcgaacg cctgaatgta tcggtacaga aactggagaa
     6961 cgccatcgag ttttatggcg ctatttcgca taacaaactg cacggcctgt cagtgcaggt
     7021 tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgccagcg aagggatcat
     7081 ccagttcttt ttcgaagaaa cgcaagatga gaatggcttt aatatctaca ttctcgacga
     7141 aagcaatcgg gttgaggtgt atcaccactg cgaaggcagc aaagaggagc tggtacgtga
     7201 cgtcagtcgc ttctactcgt catcgcatga ccgttttacc tacggctcaa gcttcatcaa
     7261 cttcaacctg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac aggtgattcc
     7321 gttccgcaca aaatctatcg gtaacatgcc gcctgccaat caggatcacg atacgccgct
     7381 attacagcag tatttttcgt gatgaacgtg ccggaaagcg aggcttatcc ggcatgcaat
     7441 cttagcggaa actgactgtt tcacccgcct gctgcgtcgc cgcctgttcc agcaaatccc
     7501 ggaaggtttc gccgctgcga tcgcaaatcc actcatcgcc tttcagatca aaatggtagc
     7561 cgccttgttt ggttgccagc catacctggt gcagcggttc ctggcggttg ataatgattt
     7621 tgctgccatt ctcaaaggta atggtcagca cgccgccgtt gatttcgcag tcgatatcgc
     7681 tgtcgccatc ccagtcgtcc agacgctctt caatggtcag ccacagttga tcagccaggc
     7741 gatgaaattc actgtcgttc attgttgtat cctgttttta agtgatggcg gcagtatagc
     7801 ggcatggggt cagggcttca aagtttgcac ctctgcggct gcgttacggc atgattcatc
     7861 cgtcaccgga ataatgatgt ctctgtgtag cgaaagattt gtctcttcat tagggcgcag
     7921 ttacaccacg cctttccctg tttctggtaa acattatgat caggtttacc gagcgagcat
     7981 cctcacgctg acggaactaa aaaagccaac aaacaaacca cattgcgata gtgcataaag
     8041 ccatcctggc gtgaggtgtt gatcacgaaa ctgccagcag aacataaatc ctcacgagta
     8101 agcgttatac tcgcagcatt tcctcacttt tcagacttca taaagagtcg ctaaacgctt
     8161 gcttttacgc cttctcctgc gatgatagaa agcagaaagc gatgaacttt acaggcaatc
     8221 cataatgaaa aacgtgttta aggcactcac tgtattactt actctcttca gcctgacggg
     8281 ctgcggtctg aaaggtccgc tctatttccc gcctgcagat aaaaatgcac cgccgccgac
     8341 caaaccggta gagacgcaaa cgcaatccac ggtgccggat aaaaacgatc gcgccactgg
     8401 cgatggtcca tcccaggtga attactaaaa gtcagtttct gtacccgcgt gattggagta
     8461 aatgatgcag ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg tcgacgcggt
     8521 aacgcagaat gtcttttttt caccggagct gattcgtcgc ctagctgatc ggcacctggg
     8581 ggtagggttt gaccaactgc tggtggttga gccgccgtat gatcctgaac tggattttca
     8641 ctatcgcatt ttcaatgctg atggcagtga agtggcgcag tgcggcaacg gcgcacgctg
     8701 ctttgcccgt tttgtgcgtc tgaaagggct gaccaataag cgcgatatcc gcgtcagcac
     8761 cgccaacggg cggatggttc ttaccgtcac cgatgatgat ctggtccgcg taaatatggg
     8821 cgaacccaac ttcgaacctt ctgccgtgcc gtttcgcgct aacaaagcgg aaaagaccta
     8881 tattatgcgc gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg gaaatccaca
     8941 ttgcgtgatt caggtcgatg atgtcgatac tgcggcggta gaaacactgg ggcctgttct
     9001 ggaaagccac gagcgttttc cggagcgcgc caatatcggt tttatgcaag tggttaagcg
     9061 cgagcatatt cgtttacgcg tttatgagcg tggggcagga gaaacccagg cctgcggcag
     9121 cggcgcatgt gcggcggttg cagtagggat tcagcaaggt ttgctggccg aagaagtacg
     9181 cgtggaactc ccaggcggtc gtcttgatat cgcctggaaa ggtccgggtc acccgttata
     9241 tatgactggc ccggcggtac atgtctacga cggatttatt catctatgaa gcaaccaggg
     9301 gaagaactgc aggaaacact cacggagctt gatgaccggg tggttgtcga ttatctgatt
     9361 aaaaatcctg agttttttat ccgcaatgcg cgcgcagtag aagcgatacg tgtgccgcat
     9421 ccggtacgcg gtaccgtttc gttggtcgag tggcacatgg cccgcgcacg taatcatatt
     9481 catgttctgg aagagaacat ggcgctgttg atggaacagg ctatcgccaa cgaaggcctg
     9541 ttttatcgct tactctacct gcagcgcagt ctcaccgccg ccagcagtct tgatgatatg
     9601 ctgatgcgct ttcaccgctg ggcgcgtgat ctcggcctgg caggtgcgag tctgcgcctg
     9661 tttccggatc gctggcgctt aggtgcgccg tcgaaccaca ctcatctggc attaagccgt
     9721 cagtctttcg aaccgctgcg tattcagcgt ctggggcagg agcagcatta tctggggccg
     9781 cttaacggac cggagctgct ggtggtacta ccggaagcga aagcggtggg atcggtggcg
     9841 atgtcgatgc tgggaagcga tgctgattta ggcgtggtgc tgttcaccag ccgcgatgcc
     9901 agtcactatc aacaagggca gggaacgcag ttacttcatg aaattgcgct gatgttgccg
     9961 gagcttctgg agcgttggat tgaacgcgta tgaccgattt acacaccgat gtagaacgct
    10021 acctacgtta tctgagcgtg gagcggcagc ttagtccgat aactctgctt aactaccagc
    10081 gacagcttga ggcgatcatc aattttgcca gcgaaaacgg cctgcaaaac tggcagcaat
    10141 gcgatgcagc gatggtgcgc aattttgctg tacgcagtcg ccgtaaaggg ctgggcgcgg
    10201 caagcctcgc attacgcctc tctgcgctac gtagcttttt tgactggctg gtcagccaga
    10261 acgaactcaa agctaacccg gcgaaaggtg tttcggcacc gaaaacgcca cgtcatctgc
    10321 cgaaaaatat cgacgtcgat gatataaatc gcctgttgga tattgatatc aacgatcccc
    10381 tcgctgtgcg cgaccgtgca atgctggaag tgatgtacgg cgcgggcctg cgtctttccg
    10441 agctggtggg gctggacatc aaacacctcg acctggagtc cggcgaagtg tgggtgatgg
    10501 ggaaaggcag caaagagcgc cgcctgccga ttggtcgtaa cgctttgtca tggattgagc
    10561 actggcttga tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt ctgtcgaaac
    10621 tgggcaagcg tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg ggcataaaac
    10681 aagggctgaa taatcacgtt cacccgcata aattacgtca ctcgttcgct acgcatatgc
    10741 tggagtcgag cggcgatctt cgtggtgtgc aggagctgct gggtcatgcc aacctctcca
    10801 ccacgcaaat ctatactcat cttgattttc aacaccttgc ctcggtgtac gatgcggcgc
    10861 atccacgcgc caaacggggg aaataatgcg tttttaccgg cctttggggc gcatctcggc
    10921 gctcaccttt gacctggatg atacccttta cgataaccgt ccggtgattt tgcgcaccga
    10981 gcaggaggcg cttacctttg tgcaaaatta ccatccggcg ctgcgcagct tccagaatga
    11041 agatctgcaa cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt atcacgacgt
    11101 gacgcgctgg cgttttcgct caatagaaca agcgatgctt gacgccggat tgagcgcgga
    11161 agaagccagt gcaggcgcac acgcggcaat gatcaacttt gctaaatggc gcagcaggat
    11221 cgacgtcccg cagcaaaccc acgacacctt aaaacagctg gcgaagaaat ggccgctggt
    11281 ggcgatcacc aacggtaacg ctcagccgga actgtttggt ttgggggatt attttgagtt
    11341 tgtgctgcgc gctggcccgc acgggcgctc gaaaccgttc agcgatatgt acttcctggc
    11401 ggcggaaaaa ctcaacgtgc cgatcggcga gatcttacat gtaggtgacg atctcaccac
    11461 tgacgtgggt ggggcaattc gcagcggaat gcaggcctgt tggatcagac cggaaaatgg
    11521 cgatctgatg caaacctggg atagccgttt actcccgcat ctggaaattt cccggttggc
    11581 atctctgacc tcgctgatat aatcagcaaa tctgtatata tacccagctt tttggcggag
    11641 ggcgttgcgc ttctccgccc aacctatttt tacgcggcgg tgccaatgga cgtttcttac
    11701 ctgctcgaca gccttaatga caaacagcgc gaagcggtgg ccgcgccgcg cagcaacctt
    11761 ctggtgctgg cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg tatcgcctgg
    11821 ctgatgagcg tggaaaactg ctcgccatac tcgattatgg ctgtgacgtt taccaacaaa
    11881 gcggcggcgg agatgcgtca tcgtatcggg caactgatgg gcaccagcca gggcggcatg
    11941 tgggtcggca ccttccacgg gctggcgcac cgtttgctgc gtgcgcacca tatggacgcc
    12001 aatctgccgc aggatttcca gatcctcgat agcgaagacc agctacgcct gcttaagcgt
    12061 ctgatcaaag ccatgaacct cgacgagaag cagtggccgc cgcgccaggc aatgtggtac
    12121 atcaacagcc agaaagatga aggcctgcgt ccgcatcata ttcaaagcta cggtaatccg
    12181 gtggagcaga cctggcagaa ggtgtatcag gcgtatcagg aagcgtgcga tcgtgcaggc
    12241 ctggtggact tcgccgagct gctgctgcgc gctcacgagt tgtggcttaa caagccgcat
    12301 atcctgcaac actaccgcga acgttttacc aacatcctgg tggacgaatt ccaggatacc
    12361 aacaacattc agtacgcgtg gatccgcctg ctggcgggcg acaccggcaa agtgatgatc
    12421 gtcggtgatg acgaccagtc aatctacggc tggcgcgggg cgcaggtgga gaatattcag
    12481 cgtttcctca atgatttccc cggtgccgaa actatccgtc tggagcagaa ctatcgttct
    12541 accagtaaca ttctgagcgc cgcgaacgcc ctgattgaaa acaacaacgg gcgtctgggt
    12601 aaaaaactgt ggaccgatgg cgcggacggt gagcctattt ccctctattg cgcttttaac
    12661 gaactcgatg aagcgcgttt tgtcgttaac cgcatcaaaa cctggcagga caacggtggg
    12721 gcgctcgccg agtgcgccat tctctaccgc agcaacgccc agtcgcgggt gctcgaagag
    12781 gcgttattgc aggccagtat gccgtaccgt atttacggcg ggatgcgctt cttcgaacgc
    12841 caggaaatca aagatgcgct ctcgtatctg cgcctgattg ccaaccgcaa cgacgacgcg
    12901 gcctttgagc gtgtggtgaa tacgccaacg cggggtattg gtgaccggac gctggacgtg
    12961 gtacgtcaga catcgcgcga tcgccagtta acactctggc aggcatgtcg tgaactgttg
    13021 caggaaaaag ccctcgccgg acgtgctgcc agcgccttac agcggtttat ggaactgatc
    13081 gacgccttag cgcaggaaac tgccgatatg ccgctgcatg tacagactga ccgggtaatt
    13141 aaagactccg gcctgcgtac catgtacgag caggagaagg gcgaaaaagg tcagacgcgt
    13201 atcgaaaact tagaggaact agtgacggca acgcgccaat tcagctacaa cgaagaagac
    13261 gaagatttaa tgccgctgca ggcgttcctc tcccatgcgg cgctggaagc gggcgagggg
    13321 caggcggata cctggcagga tgcagtgcag ttgatgacgc tgcactcggc gaaaggcctg
    13381 gagttcccgc aggtgtttat cgtcggtatg gaagagggca tgttcccaag ccagatgtcg
    13441 ctggatgaag gcggacgtct ggaagaagaa cgccgtctgg cctacgttgg tgtaacccgc
    13501 gcgatgcaga aactgacgct gacctacgcg gaaactcgcc gtctgtatgg caaagaggtt
    13561 taccatcgcc cgtcgcgctt tatcggtgag ttgccggaag agtgtgtgga agaggtgcgc
    13621 ctgcgcgcca cggtaagccg cccggtcagc catcagcgta tgggtacgcc gatggtcgag
    13681 aacgacagcg gctacaagct cggccagcgt gtacgccacg ctaagtttgg tgaaggcact
    13741 atcgtcaata tggaaggcag cggcgagcat agccgtttgc aggtggcatt ccagggccag
    13801 ggtattaaat ggctggtggc ggcatacgcc cggctggaga cggtataacg ttgcgggatg
    13861 cggtgcgcca caccgcatcc ggcatcagca ccttacttat cacatttctt cacatcaata
    13921 ttccagtcag caccagacgc aacctgatgt ttctgcgcga aattatccat attcagcgcc
    13981 acggaaacat tcaataagct gttcagatac accatcggct gtccttccgg cacatcgccg
    14041 aagcttgcga catacggcat tttccctgcg tatttttgcc gggaaccttc ggaaatggtc
    14101 acacacaacg tgtcgttgag tttgatcccc gcctgattca gcaactcgtc gctgatattg
    14161 ctccagacgt tgccgtactg aatatcgaga atcgggatat tgcccttcac ttcacctttt
    14221 gttgcttttg ctttctgata agaaagttcg acaacctttg caggaagctc aggccccacc
    14281 tgctcgaacg tgatcgcccc ggaagccaga cgtgcaccgg tgtaggcata cacgtcacga
    14341 ccgtggaagg tatatgactt ttcagaacct ttcaggcggt tagctttttc gtcgatttcc
    14401 cggacgctct caatacccag cgattccgcc accaaagtga gcgtaccgtt atccggtgaa
    14461 acgaagtatt gaccgttttt ggttttcagc actatcgatt tacggtcagt gcctacgccc
    14521 ggatcgacca cactgacaaa taccgaacct tgcggccagt aactggcggt ctgatacagg
    14581 cggtaagcgc cttcccagat gttatacggt ggaatttcgt gtgttaaatc aaatatttta
    14641 agattatggt caacaccgaa tgcgacccct ttcatcgccg agacggcacc atctttcagg
    14701 ctaaaatcag tttgtaaaat caaagcgtta tccgcataag cggatgatga tcccagacac
    14761 gttaataaaa ataacgttgc gagcgttttt ttcatttttg tttcccttca tttatctgat
    14821 gattttccag ctgtgtagcc agccccagtt tgtctggcct cctcgagtgt acaatgctta
    14881 acctttccgc tcgacggaaa tcatggtgac aaaaggatag cgttgccacg ggattgcccc
    14941 gcctttcata tacatatgtg aaatcgtgcc atcaagataa agcagctgct caacgttcag
    15001 tttcgctttg gcataacagg caaaatcata aaaatttgtc gcctgctggc tcaacaaaaa
    15061 cacggcattc ccatgtttat taattccaac accgttacga attttacgtg aggcgacgtt
    15121 gggatgaata cgcggattaa ttacactgtt ttccatcaac attggccctg actgcaccgc
    15181 aaactgaatc tctttactgg ttttgaaggc atccagacga acgatgccga ctttatctcc
    15241 cgcgacataa aacacgccgc caggacggat aaagaaattc ccttcacctg aagcgagatt
    15301 taacgccacc ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc
    15361 atagatgccg ccgttcatcg ccatctgcac ctgaccctga ctattcatat ccgccagcag
    15421 agcatgtaaa gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc
    15481 tgtttgagga ttaacggtat acgcctgtac ggtcagcgtt ggatctgaga gtgcgcaatc
    15541 atcagcggca acagcaaaca agggaagtaa ggtgagggcg agaaaaatcc gtttgagatt
    15601 caaggtgatc attcctttac caatgagtag ccgatgcgcc attataggtc ctggatgtgg
    15661 gattttttta tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg
    15721 ttctgtggtg ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg
    15781 atcactctaa gaggacattc gccttggaca cacccagtag atactggctc actatcctgt
    15841 catccaggat caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg
    15901 acctcaattt ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt
    15961 tctgtgtgcc caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc
    16021 gcatttcaac tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt
    16081 gtaaatgcag tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa
    16141 tctgaacttg gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca
    16201 cgtttctttg aagacgatga cggcctgcat attcactcct tcttcttctt tgaagatgcg
    16261 gaagatcacg ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact
    16321 ctgcgtgagc gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg
    16381 atggtagacg gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag
    16441 ttggcagatg aaattgaaaa tatctatagc gacctggagc aattgagccg ggtgattatg
    16501 gaagggcatc agggcgatga gtacgacgag gcgctctcca ctctggcgga actggaagat
    16561 atcggctgga aagtgcgcct gtgtctgatg gatacccagc gcgcgctcaa cttcctggta
    16621 cgtaaagcac gcttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgagatatc
    16681 gaatccctgc tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg
    16741 gcaatgggtt ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc
    16801 gtcgtgttcc tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg
    16861 ccagaactga agtggagttt cggctaccca ggcgcgatta tctttatgat cctcgcgggc
    16921 ctggcaccgt atctgtactt taagcggaag aactggttgt aatatgagtg ccggataaag
    16981 ctattttttt atccggctta tttctaaaaa ttgtcttttt taaagttatg cggattgaaa
    17041 acttgctcta ttttattttc ttgtgttaat tgatactcag taaaatcgtt aataccaata
    17101 tgagtgttac gataagggta ttcattaata accattaagt aatttttcgt gatgcaatta
    17161 tcattgctct taattgtcgg g
//
LOCUS       DAZKFZ010000087        17076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000087 DAZKFZ010000000
VERSION     DAZKFZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            46..1185
                     /gene="arnB"
                     /locus_tag="ACHOQW_002613"
     CDS             46..1185
                     /gene="arnB"
                     /locus_tag="ACHOQW_002613"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP9573675.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1189..2157
                     /gene="arnC"
                     /locus_tag="ACHOQW_002614"
     CDS             1189..2157
                     /gene="arnC"
                     /locus_tag="ACHOQW_002614"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP9573676.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2157..4139
                     /gene="arnA"
                     /locus_tag="ACHOQW_002615"
     CDS             2157..4139
                     /gene="arnA"
                     /locus_tag="ACHOQW_002615"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP9573677.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4136..5026
                     /gene="arnD"
                     /locus_tag="ACHOQW_002616"
     CDS             4136..5026
                     /gene="arnD"
                     /locus_tag="ACHOQW_002616"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP9573678.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5026..6678
                     /gene="arnT"
                     /locus_tag="ACHOQW_002617"
     CDS             5026..6678
                     /gene="arnT"
                     /locus_tag="ACHOQW_002617"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP9573679.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6675..7010
                     /gene="arnE"
                     /locus_tag="ACHOQW_002618"
     CDS             6675..7010
                     /gene="arnE"
                     /locus_tag="ACHOQW_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP9573680.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7010..7396
                     /gene="arnF"
                     /locus_tag="ACHOQW_002619"
     CDS             7010..7396
                     /gene="arnF"
                     /locus_tag="ACHOQW_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP9573681.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7390..7656)
                     /gene="pmrD"
                     /locus_tag="ACHOQW_002620"
     CDS             complement(7390..7656)
                     /gene="pmrD"
                     /locus_tag="ACHOQW_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP9573682.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7766..9121)
                     /gene="menE"
                     /locus_tag="ACHOQW_002621"
     CDS             complement(7766..9121)
                     /gene="menE"
                     /locus_tag="ACHOQW_002621"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP9573683.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9118..10080)
                     /gene="menC"
                     /locus_tag="ACHOQW_002622"
     CDS             complement(9118..10080)
                     /gene="menC"
                     /locus_tag="ACHOQW_002622"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP9573684.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10080..10937)
                     /gene="menB"
                     /locus_tag="ACHOQW_002623"
     CDS             complement(10080..10937)
                     /gene="menB"
                     /locus_tag="ACHOQW_002623"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP9573685.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10952..11710)
                     /gene="menH"
                     /locus_tag="ACHOQW_002624"
     CDS             complement(10952..11710)
                     /gene="menH"
                     /locus_tag="ACHOQW_002624"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP9573686.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11707..13377)
                     /gene="menD"
                     /locus_tag="ACHOQW_002625"
     CDS             complement(11707..13377)
                     /gene="menD"
                     /locus_tag="ACHOQW_002625"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP9573687.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13466..14761)
                     /gene="menF"
                     /locus_tag="ACHOQW_002626"
     CDS             complement(13466..14761)
                     /gene="menF"
                     /locus_tag="ACHOQW_002626"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP9573688.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14840..15145)
                     /gene="elaB"
                     /locus_tag="ACHOQW_002627"
     CDS             complement(14840..15145)
                     /gene="elaB"
                     /locus_tag="ACHOQW_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP9573689.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15200..15661)
                     /locus_tag="ACHOQW_002628"
     CDS             complement(15200..15661)
                     /locus_tag="ACHOQW_002628"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9573690.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15726..16643
                     /gene="rbn"
                     /locus_tag="ACHOQW_002629"
     CDS             15726..16643
                     /gene="rbn"
                     /locus_tag="ACHOQW_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP9573691.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16831..>17076
                     /locus_tag="ACHOQW_002630"
     CDS             16831..>17076
                     /locus_tag="ACHOQW_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFP9573692.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSI"
BASE COUNT         3985 a         4615 c         4355 g         4121 t
ORIGIN      
        1 tagcaaatat aagtggacag gtattcaatg gcggaaggaa aagcaatgtc agaatttttg
       61 cctttttcga gaccagcaat gggcgtggag gaactcgctg cagttaaaga ggttctcgaa
      121 tccggttgga tcacaaccgg tccgaagaat cacgcgcttg aacaagcctt ttgccagttg
      181 acgggaaatc agcatgccat cgcggtcagt tcagccaccg ccggaatgca tatcacgcta
      241 atggcgttgg aaattggcaa gggcgatgaa gtgattacgc cttccctgac ctgggtttca
      301 accctcaata tgatttcctt gcagggtgca acgccggtaa tggtggatgt cgatcgcgat
      361 acgctgatgg tcacgcctga agcgatcgaa tcagccatta cgccacgcac taaagccatc
      421 attccggtgc attatgccgg tgcgccagca gatattaacg ccattcgcgc cattggcgaa
      481 cgttacggca tcgcagttat cgaagatgct gcccatgccg taggtacgta ttacaaaggg
      541 cgacatattg gcgcaaaagg taccgctatt ttttcatttc atgccattaa aaatattacc
      601 tgtgcggaag gaggcctgat tgttactgat aatgaaaata ttgcccgcca gctacggatg
      661 ctgaaatttc acggcctggg tgtcgatgcc tatgacagac aaacctgggg ccgtgcaccg
      721 caggctgaag tcttaacgcc gggctataag tacaatctga ccgatattaa cgccgcgatt
      781 gccctgacac agttagccaa attagagcac ctcaacaccc gtcggcgcga aattgcccag
      841 caatatcagc aagcactggc agctctcccc tttcagccat taagccttcc cgcctggccg
      901 cacgttcacg cctggcatct gtttattatt cgtgtcgatg aacaacgttg tggtatcagt
      961 cgcgatgcgt tgatggaagc gttaaaagaa agaggtattg gtaccgggtt acatttccgc
     1021 gccgctcaca cacaaaaata ttatcgcgag cgttttccct cgctgtcgtt accgaatacc
     1081 gaatggaata gcgaacgcat ctgttctttg ccgctgttcc cggatatgac taccgccgat
     1141 gccgaccgtg tcatcacagc ccttcagcaa ctcgcaggac aataagccat gtttgaaatc
     1201 caccctgtta agaaagtctc ggtggttatt cccgtttata acgagcagga aagcttaccg
     1261 gaattaatca ggcgcaccac cacagcctgt gaatcgttgg ggaaagagta tgagatcctg
     1321 ctgattgatg acggcagtag cgataattcc gcgcatatac tggtcgaagc ctcacaagcg
     1381 gagaacagcc atattgtatc tattttgctt aaccgcaatt acgggcaaca ttcagcgatt
     1441 atggcgggat tcagtcacgt tactggcgac ttaattatta cccttgatgc cgatctccag
     1501 aatccgccag aagaaatccc ccgcctggtg gcaaaagccg atgaaggtta cgacgtggta
     1561 gggactgtac gccagaaccg ccaggacagc tggtttcgta aaacagcttc gaagatgatt
     1621 aaccggctta ttcagcgcac caccggcaaa gcgatgggtg actacggttg tatgctgcgc
     1681 gcctatcgcc gtcatattgt cgatgcgatg ttgcactgcc atgaacgcag cacctttatc
     1741 ccgattctgg cgaatatctt cgcccgccgt gccattgaaa ttccagtgca tcatgccgag
     1801 cgtgagtatg gtgaatccaa atacagcttt atgcgcctga ttaatttgat gtacgaccta
     1861 gtgacctgcc ttaccacaac gccgctacgt atgctgagtc tgctcggcag cattattgcg
     1921 attggaggtt ttagcattgc ggtgctgctg gtgattttac gcctgacctt cggaccacaa
     1981 tgggcggcag aaggcgtctt tatgctattt gccgtgctgt ttacttttat tggcgctcag
     2041 tttatcggca tgggattact cggtgaatat atcggcagga tctacaccga tgtccgcgcc
     2101 cgcccccgct attttgttca gcaagttatc cgtccatcca gcaaggaaaa tgaataatga
     2161 aaaccgtcgt ttttgcctac cacgatatgg gatgcctcgg tattgaagcc ctgctggctg
     2221 ccggttacga aattagcgcc atttttaccc ataccgataa tcccggtgaa aaagcctttt
     2281 atggttcggt ggctcgtctg gcggcggaaa gaggcattcc ggtttatgcg ccggataacg
     2341 ttaatcatcc gctgtgggtg gaacgcattg cccaactctc accagaggtg attttctctt
     2401 tttattatcg ccatcttatt tgcgacgaga ttttgcagct cgctcccgca ggtgcattta
     2461 atctgcacgg ttcgctgtta ccaaaatatc gtggtcgcgc gccgctgaac tgggtgctgg
     2521 tgaacggtga aacagaaact ggcgttacat tgcaccgaat ggtaaaacgt gccgatgccg
     2581 gggccattgt ggcgcaactg cgcgttgcca ttgcgccaga cgatatcgct attacgctgc
     2641 atcataaatt gtgccatgcc gcgcgccagc tactggaaca gacattaccc gccattaaac
     2701 acggtaatat tctggaaatc gcccagcgcg aaaacgaagc cacctgtttt ggtcgcagaa
     2761 cgccggatga cagtttcctc gaatggcata agccggcatc cgtactgcac aacatggtac
     2821 gtgccgttgc cgatccgtgg ccgggtgcct tcagctatgt tggcaatcag aaattcaccg
     2881 tctggtcgtc gcgtgttcat cctcatgcca gcaaagcaca gccggggagc gtgatttctg
     2941 ttgcgccact gctgattgcc tgtggcgatg gcgcgctgga aatcgtcacc ggacaagcgg
     3001 gcgacggcat tactatgcag ggctcgcaat tagcgcagac gctgggcctg gtgcaaggtt
     3061 cacgcttgaa tagccagcct gcctgcaccg cccgacgccg tacccgggta ctcatcctcg
     3121 gggtgaatgg ctttattggc aaccatctga cagaacgcct gctgcgcgaa gatcattatg
     3181 aagtttacgg tctggatatt ggcagcgatg cgataagccg ttttctgaat catccgcatt
     3241 ttcactttgt cgaaggcgat atcagtattc attccgaatg gattgagtat cacgtcaaaa
     3301 aatgtgatgt cgtcttgccg ctggtggcga tagccacgcc gattgaatat acccgcaacc
     3361 cgctgcgcgt atttgaactc gattttgaag agaatctgcg cattatccgc tactgcgtga
     3421 agtaccgtaa gcgaatcatc ttcccgtcaa cttcagaagt ttatgggatg tgtagcgata
     3481 aatacttcga tgaggaccat tctaatttaa tcgtcggccc ggtgaataaa ccacgctgga
     3541 tttattcggt atcaaaacaa ttacttgatc gggtgatctg ggcctatggc gaaaaagagg
     3601 gtttacagtt caccctcttc cgcccgttta actggatggg accacgactg gataacctta
     3661 atgcggcacg aatcggcagc tcccgcgcga ttacgcaact cattctcaat ctggtagaag
     3721 gttcaccgat taagctgatt gatggcggaa aacaaaaacg ctgctttact gatattcgcg
     3781 atggtatcga ggcgttatac cgcattatcg aaaatgcggg aaatcgctgc gacggcgaaa
     3841 ttatcaacat tggcaatcct gagaacgaag cgagcattga ggaactgggc gagatgctgc
     3901 tggcgagctt cgaaaaacat ccgctgcgcc atcatttccc accgtttgcg ggctttcgcg
     3961 ttgtcgaaag tagcagctac tacggcaaag gatatcagga cgtagagcat cgtaaaccga
     4021 gcatccgcaa tgcccaccac tgcctggact gggagccgaa aattgatatg caggaaacca
     4081 tcgacgaaac gctggatttc ttcctgcgca ccgttgatct tacggataaa ccatcatgac
     4141 caaagtaggc ttacgcattg atgtcgatac ctttcgtggc acccgtgaag gcgtgccgcg
     4201 tctgctggaa atcttgagta agcataatat tcaggccagc atttttttca gcgtcggccc
     4261 ggacaatatg ggccgccatc tctggcgact ggtgaagcca cagtttttgt ggaagatgct
     4321 gcgctcaaac gcggcatcgc tttatggctg ggatatttta ctggcaggta cggcctggcc
     4381 aggtaaagag attggtcatg ccaatgccga tatcattcgt gaagcggcta aatatcacga
     4441 agtcggcctg cacgcctggg atcaccatgc ctggcaagcc cgtagcggta actgggatcg
     4501 gcaaacaatg atcgacgata ttgcacgcgg ccttcgcact ctggaagaga ttatcggtca
     4561 accggtaacc tgttctgccg ctgcgggctg gcgtgccgac cagcaggtga tcgaagcaaa
     4621 agaagcgttc catttgcgct acaacagcga ttgtcgtggg gccatgccgt tccgtccact
     4681 gctcgaatca ggaaaccctg gcactgcgca aattccggtg accttaccca cctgggatga
     4741 agtgattggt cgggatgtga aagcagaaga ttttaacggt tggttactca accgcatcct
     4801 gcgagataaa ggcacgccgg tttataccat tcatgcagaa gttgaaggct gcgcttatca
     4861 gcataatttt gtggatctcc tcaaacgcgc agctcaggaa ggcgtgacat tttgcccttt
     4921 aagcgaactg ttatcagaga cgttgccgct cggacaagtt gttcgcggaa atattgccgg
     4981 acgtgaaggc tggctgggtt gccaacaaat tgcgggtagt cgctgatgaa atcggtacgt
     5041 taccttatcg gcctcttcgc gtttattgcc tgctattacc tgttaccgat cagcacgcgt
     5101 ctgctatggc aacccgatga aacgcgttat gcggaaatca gtcgagaaat gctggcatcc
     5161 ggcgactgga ttgtgcccca tctgttaggg ctacgttatt ttgaaaaacc cattgccgga
     5221 tactggatta acagcattgg gcaatggcta tttggcgcga ataactttgg tgtgcgggca
     5281 ggcgttatct ttgcgaccct gttaactgcc gcgctggtga cctggtttac tctgcgctta
     5341 tggcgcgata aacgtctggc tctactcgcc acagtaattt atctctcatt gtttattgtc
     5401 tatgccatcg gcacttatgc cgtgctcgat ccgtttattg ccttctggct ggtggcggga
     5461 atgtgcagct tctggctggc aatgcaggca cagacgtgga aaggcaaaag cgcaggattt
     5521 ttactgctgg gaatcacctg cggcatgggg gtgatgacca aaggttttct cgcccttgcc
     5581 gtgccggtat taagcgtgct gccatgggta gcgacgcaaa aacgctggaa agatctcttt
     5641 atttacggct ggctggcggt catcagttgc gtactgacgg ttctcccctg gggactgacg
     5701 atagcgcagc gggagcctga cttctggcac tattttttct gggttgagca tattcaacgc
     5761 tttgcactgg atgatgccca acatagagct ccgttctggt actacgtgcc ggtcatcatt
     5821 gccggtagtc tgccgtggct gggattactc cccggtgcac tatacacagg ctggaaaaac
     5881 cgcaagcatt ccgcaaccgt ctatttgttg agctggacga taatgccgct gctgtttttc
     5941 tccgttgcta agggtaaatt gcccacctat attctttcct gctttgcacc tttggcaatg
     6001 ctgatggcgc attacgcttt gctggcagca aaaaataatc ctctggcgct gcggattaat
     6061 ggctggatta acatcgcttt tggcgtcact ggcattattg ccacatttgt ggtctccccc
     6121 tggggaccaa tgaacacgcc ggtgtggcaa accttcgaga gctataaagt cttttgtgcc
     6181 tggtcgattt tttcgctatg ggcatttttc ggctggtaca ccttaacaaa cgtcgaaaag
     6241 acctggtctt ttgccgcgct ttgcccgctg gggctggcgt tgctggtagg attttcaatt
     6301 cctgacagag tcatggaagg aaaacatccg caattttttg tcgagatgac acaagaatca
     6361 cttcagccaa gccgctatat tcttactgat agcgtcggtg ttgccgcagg tctggcatgg
     6421 agtctgcaac gcgatgacat catcatgtat cgccagacag gtgagttgaa atacggcctt
     6481 aattatccgg atgcgaaagg gagatttgtc agcggtgatg agttcgcaaa ctggcttaat
     6541 caacatcgtc aggaggggat tattacactc gtgctttcgg ttgaccgcga tgaagatatc
     6601 aacagtctcg ccattccgcc cgcagatgcc atcgatcgtc aggagcgtct ggtgctgatt
     6661 cagtatcgtc ccaaatgatc tggctaacat tagtctttgc cagcttgctt agcgttgccg
     6721 ggcagttgtg tcagaaacag gcaacctgct ttgtggcgat aagcaaacgg cgcaaacata
     6781 tcgtgctgtg gctgggactg gcgctggctt gtcttggtct tgccatggtg ctctggctgc
     6841 tggtcttgca gaacgtaccg gtaggtattg cctacccgat gttaagtctg aattttgtct
     6901 gggtgacgct ggctgcagta aaactgtggc acgaaccggt atcgccgcgt cactggtgtg
     6961 gggtggcgtt cattattggt ggcattgtga tcctcgggag tacggtgtaa tgggcctgat
     7021 atggggatta ttcagcgtca ttattgcctc agttgcgcaa ttaagcctgg gttttgcggc
     7081 gagtcatctg ccgccgatga cgcacttatg ggattttatc gcggcgctgt tagcttttgg
     7141 gctggatgcc cggatactgt tactcgggct gctgggttat ctgctttccg ttttttgttg
     7201 gtataaaaca ctacataaac ttgcgctaag caaagcctat gccttgctca gtatgagtta
     7261 tgtgctggta tggattgctt caatggtact tcccggctgg gaagggactt tttcgctgaa
     7321 agcactactg ggagtagcct gtattatgag cgggttgatg ctgattttcc tgcccacgac
     7381 aaaacaacgt tactgagttt tccctgccac tttacactgc cgttcccact catcggggct
     7441 ataacagctt gcagagagga ctttcaccgt atgcagcttt tcacgattaa tacaataaag
     7501 cgcattctgc aaaggcgaga gtaaatcccc cactttcacg gcgaagtcac tcttcacttc
     7561 ggcgatcatt tttatcgcgc cgccagcatc gcagagcata agtacatgtc tgttatcttg
     7621 tttattgcag cacgattttt tgaccagcca ttccattgta ttatcctgtt tgctaagagt
     7681 tttcatatca atttacagat taacagataa actctttatc aacggcaatg gaatggcaga
     7741 actgagaagt gaaacggagg gttaattatt gctgacgttg cacccactct tttagcgcct
     7801 gacgtgaaat tttaataccg ccgtttttca gctccggcgg caaagtcagc caacgtaccg
     7861 gttgctgaaa acgggccagc ttatctttca cccattcact aagatcaacg ctctcgtggt
     7921 catactccat caccgccacc ggtcgatgac caaactcctt gtcggcaacg gggacgataa
     7981 acacctgcaa aaccgcagga tgtgcagcaa ttacgcgctc gacttcttcc gtctgaatac
     8041 cctctccgcc actgaagaat agattgtcta aacgtccgac aatggtcagc ttgccattat
     8101 gcatctcacc gcgatcgcgc gtagcgtacc agccttcgtc attaaccagt gaaaccagtt
     8161 gcccgttacg ccagtaacct tctgccatac tggcagcccg cagccacact tcattattaa
     8221 cgattttcac ttcccgaccc ggcagcggcg aaccaacgtc tgccaggccg tcggcttctt
     8281 tcgcacacac cgtggaggca aactcggtca gaccatagcc gcaaaagcaa cgaatcccct
     8341 gctcgcgcgc ctgttccgtc aactcgaccg ggatagccgc gccgccaagt aacaccgctt
     8401 tcagggaaac ggaactgcgg ttaaccagca aacgccagag ttgtgttggc accagtgaag
     8461 cgtgagtaca gcctgccagc atttgctcca atggctgttt atcacgcacc gtcatccgcg
     8521 caccagcgta taaccagcgc cacataattc cctgaccgga gacgtgaaac agcggtaaag
     8581 agagcaacca gtcatcgtga tcgccaaacg gaatcagcga taacacccct tcggcactgg
     8641 caagatgggc ctgataagta tggacagcgg ctttcggcaa cccggtagaa cccgaggtca
     8701 acgtcattga gcacagacgc gtcggctgcc acgtagcgac atgtgcgcct tcaacccgct
     8761 gaatgtgcag cgacgttaat gccggaaacg tgttttcccc atccggcacc agcgcaaatt
     8821 gcagcgtcag attgggcagc aattcttcaa gcaacggttg cggcagctga gggttcacgg
     8881 gcaacacccg cgccccgcat tgcagtaacg ccagccaagc gagcagcgtt tgcggcgtat
     8941 tccacgcccg caacatcacg ccgctgtcct caaccacacc ctgcaccgca aatccggagg
     9001 ctaattcatc gacgcgagca caaagctcgc gccagttgag ttgctcgtca ttaagacgta
     9061 aggcgatggc ttctccccgc acttgccgcc agtgacgcca cggccagtca gagaagatca
     9121 taacaaccgc tccagtgcat caacttccac gacaggcagc gtgctacccg gccagcgacg
     9181 tacctgctgc gcctgcatta gatccagcgt gtccagccct ggaatggtgt ccggcgttaa
     9241 ccaggcagca atccgcgcca gttgcgttaa gcctaagctc gattcaatgg aagaactgat
     9301 cactgccgtc agccccagcg cgtgcgccgc ctgtacctgc tcgcgaactt tctccagact
     9361 gcccgtgagc gtgggtttga taactaccgc gcgcacgccc tcttcagcca caaaggcaaa
     9421 atccggctcg cgcaagcttt catcccaggc aatggcaatg ccggtttcac gggcaaacgc
     9481 tcgcgaatca tcgcgggttt tgcacggctc ttcgagaaac gcgatgcggt ggcgataatc
     9541 cgggttaacg tatttggcaa actgctgacc tttcagcggt gtccaggcgc gatttgcgtc
     9601 aagacgcaaa tgcagatccg gaattgcctc cagcaacaga ttcaccacca tgccgtcgcg
     9661 caccgcttcg tacaatccca ctttgacctt cgccactttc tcgcctggca tatctgcaag
     9721 tttgaggatc agatcgtccg gatcgccatt acacagtggt gccgtacggt aattggccgc
     9781 ctgcgggagc gtatctgcca gctccgccaa tgcacaactt acgccaaagg caacggaagg
     9841 catctgcggt aattcgcaat cgcctgccag ccagttattt acccaggcaa gcagcacact
     9901 ttgcgcctct tcccaggttt cctgactgaa gcccggcagt ggggagatct ccccccaccc
     9961 ttcgcgctcg ccctcacgca ggcaaacgta cagcccgtcg cgggttttta accgcctgtc
    10021 gcgcagaacc acccccgcgt ccatggggat ctgccagcgg tatacctgcg cgctacgcat
    10081 tacggattcc gtttgaattt gctgaagtca ggctgacgtt tctggttgaa ggcgttgcga
    10141 ccttcctgac cttcttccgt catgtagaac agcatggtgg cgttgcccgc cagctcctgc
    10201 agacctgcct gaccgtcaca gtcggcgttc agtgcagctt tcaggcagcg cagcgccatc
    10261 ggactgtttt gcagcatttc acggcaccag cgaacggttt ctttttccag atccgccagc
    10321 ggtaccacgg tgttgacaag gcccatatcc agcgcctgtt ttgcgtcgta ctgacggcac
    10381 aggaaccaga tttcacgcgc ttttttctgc ccgacgatac gagccatgta ggaagcgccc
    10441 cagccgccgt cgaaggaacc gactttcggg ccagtctgac cgaagatggc attatctgcc
    10501 gcgatagtca ggtcgcacat catgtgcaga acgtgaccac cgccgatgga atagccagcc
    10561 accatcgcga caaccggttt cggacaggta cggatctgac gctggaagtc cagcacgttc
    10621 aggtgatgta cgccggaatc atctttatag ccgccgtaat caccacgcac tttctggtca
    10681 ccaccggagc agaacgcttt atcgcctgcg ccagtcagaa tgatcacgcc gacgttatcg
    10741 tcataacgcg catctgccag tgcctggatc atctctttta ccgtcagagg acggaaggca
    10801 ttgcgcacct gcggacgatt aatggtgatt tttgcgatac cgtcggtgga tttttcataa
    10861 cgaatgtcct caaagccttc ggagcagtcg tgccattcaa ccggtgcgta aagcattgct
    10921 tcatcaggat aaatcatatt gtgtccttta gtcagaaacg caagatctgc gccagacttg
    10981 cgattacgcc agcgggattt tcccgatgcg cgttatgtcc ggcgcgagga atgacatggc
    11041 agtcggcagc cagttccgcc gccagggcgc ggaatttgct gtcacgttca ccacataaat
    11101 aataaaacgc aaatgtgcgg gcgctaaggt tagcacgtaa atcaggctgg acggcgagag
    11161 aagtcgcctc cagcatggcg gcaagggttg cgccattatt gttgctgcgc agcaccacca
    11221 gctcccggcg ttgatcgtca ttgagcgagg caaaaacagg ctgttgatac cagtcggcaa
    11281 ataccgcggt taatggttcg gaacgaaaac gctgcgccca ttggcgatcg gaacgctgac
    11341 gttccgcgcg ttgttcagca ttttgcagcc ccggatgacc gccttcgaca acaaccccac
    11401 aaagccccgc cagcccctgg caagcggcca tcatcgccac ccgtccgcca agcgagtacc
    11461 ccaccagcca gaagctaagg atgttgtaac taaccagggt tttacgcagt aagtcggtga
    11521 catcatcaaa tccatcgacg ctaatcgccg ccgaaccacc gtgacctggg agatcaacat
    11581 acaaccgtga gtagtcggca aacgcctcgc cgacttcctg ccattcgagg caatcgccgg
    11641 aaaaaccgtg caaaaacacc agccagggta aacctggttt gccgtgtttt gcctgcgcgt
    11701 gcaggatcat aaatggctta cctgcgccag cagctgctgg agcgtttgcg cgccgtcggt
    11761 gtcgttaacc accatttcaa ttaccgtggt ggtcggagta cgccaggcgt cggcaagtac
    11821 cgtttcaagt tcctgccagt tttgcggacg atgatatttc agctcaaaca tcgcggcagc
    11881 gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc tcgcgctcgc ttttcggcgt
    11941 tggcagcagc gagaaaattt gcccgccgtt gttgttcacc acaattaata ccagcggcgc
    12001 ggaaacctgg cgcaataacg ccagcgcatt caggtcgtaa agtgcggaga gatcgcccac
    12061 aatcgccagc gtcggtttgc cgcttgcccg ctgaacgccg gcggcagtcg agagcagccc
    12121 gtcgataccg ctggccccac ggttgctgta caccgggtaa cctgccggaa gttgcgaaag
    12181 cgcatcaatc agacgtacca ccaggctgtt accgacaaac aattgcccct gttcaggcag
    12241 atagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac gcatcgcgac aggcaataac
    12301 cgcctgcatt gcctgttccg ccaggcgcgg gatttcaacg caccagggct ggcgtttttc
    12361 tgccggatgc agttccagcc agtcggcaat attggcaatt aagcggcgtc cacgatggtg
    12421 tgccggatca agccgccctt cgatgtcatc cacaatccag tactcttcgg gttcacagct
    12481 tgcctgccat tgcaggagcc gtttgcccgt caggctgctt cccagttgca ccacaatttg
    12541 cgcctgttgc agttcgctgg tcgctttggc attgcctaac cagagatcgg cacacggcag
    12601 cggctgtccg gtttgcgaca gcacgtcgcc aatcagcggc cagccaagag tttgcgccca
    12661 cagggcaact tttttgccct cttccgcact catgcgcccg gcaaccacca cgccgcgctt
    12721 ttgccgccag aagaaccagt cgcgctgttt ttcgctctca agacgaggcg cttcacgcag
    12781 ccacggctta tcatcctgcc accagtcacc cagacgctgt tgccagctaa gcccagtgtc
    12841 gtccatctcg ccatacagcg gttcagcaaa cgggcagttg atatggaccc caccagcatg
    12901 aagcgtaccg agagcatggt cgatggtaga aaccagccaa cgtgcgggga tatcctgggt
    12961 cgggcgcggc agtgaaatac tgtgcgtggg gtgagaggcg aacattcccg gctgacgaat
    13021 cgcctgattc gcgccgcagt caattagctc cggcgggcga tcggcggtta acaggatcag
    13081 tttttctccg gttaacccag cttcaatcag tgccggatag agatttgcta ccgccgtgcc
    13141 ggaggtcaca atcaccgcca ccggctgctt gctgactttc gccagcccca gcgccagatg
    13201 ccccagtcca cgctcatcga aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc
    13261 taacgttaac ggtgtagaac gcgagccagg ggcgatgcag atgtgtctta cgccgtgacg
    13321 cgttaatgct tccagaatga ccgccgccca gcgtcggtta aatgcgctta ctgacatgag
    13381 tttgtccggt atcaatattg cggctaagta taaggagcta caaaaataga ttattgatat
    13441 gaatcggtaa tgatgcgact cattactatt ccatttgtaa taaagtacgc agccctgccg
    13501 ctttgttgtc gatttcctgc cactcttgct cggggtcgga accacggaca atgcccgcgc
    13561 cagcatatag ccgcacgaca ttgccgctga tttttgctga gcgcagggaa acgcagaatt
    13621 cgctttgttg taatgagaga tagcccgcag aaccggcgta ccattctcgg gtgaacggtt
    13681 cgtgatgggc gataaactgt cgcgccagat cgcgcggtaa gccagcaact gctgccgttg
    13741 gctgcaactg atgtaaacag atcacatcat ccgctttgtt gagtgaagtc cagatacagc
    13801 ggcgaagatg ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca tccagcgtct
    13861 gggtatcggc ctgtaatcgt tggcagatat cttccaccac cagcatgttc tcgcgctggt
    13921 ttttatcatc cgccatcagc cactctccta actgctgcgc ctgcttatca tcaggatgat
    13981 ttgctactgt tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc cgccataacc
    14041 gttccggtga agagccaaga aaagcatttt cgccatcaaa ggccatgtaa aaatggtagc
    14101 aattgagatt cagtcgacga ctggcagcca tcatcgccgc cgcattgacc ggacttgcga
    14161 aatgcaggtc agttgcccga gcaagcacca ctttgtcgag ctcaccttcg gcgatggttt
    14221 tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt tgttctcgcg
    14281 tagtggttaa atgtaaccca ggcaagggct tgatactcac cagtgtggcg ataaattctc
    14341 ttgccttaat cgcatcgtgc tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg
    14401 ctttaccgcc acagcgtcgc cattccaggc gtggtaaaag taaattgccc tgcgacgggt
    14461 caaacgcatt cagcccccaa atgcgtaagt cggcgtgttc cgggtgctgg cgaagaaaac
    14521 gttgtgcctg gtccaacgac gtaaaacggg taatcgcgcc caggacggca gcttcttcat
    14581 caccattacg ttgttgccag tagaattgcg ggtacgtttg ctgactggcc agccagctca
    14641 aggcatcaaa agcgtctttg agagggaaag gaatatcgat aacccgaatg ccgggtgtcg
    14701 ccggaatctc ttgcgacaaa tggcgcagta gattttccag cgccgtagta agtgattgca
    14761 cgcggacctc tccctgttaa aagcctcaca ttatacgggg tactacaaaa aaaatgcagt
    14821 accccggtgt agggaggttt taacggcgtg ccagcaacag tcctagtacc agcccaacgg
    14881 ccgcacccac accaattccc tgccagggtt tttcgtggac gtagtcatca gcacgataaa
    14941 cagcctgctt cgcccgatag taataactgt ctgaagcctg gctaacccgt tttttcacat
    15001 catccagcgc tttttctgca cgcgctttca gctcaacata tttctgatcg gcgggatcgc
    15061 cagaggagcg gagcacctct tccagtgttt cactaagcag cgtcaggtcg tcatcgatac
    15121 gtgtatcacc aaactgatta gacattctcg ttctccattt gcgtaaaacc tgtcactaac
    15181 tatagacaag ggttattgat tacgcctgaa ttacctcgcg tgccatccca atgtgtggga
    15241 taccatcttc ttcatagatc tccgtcacag ggataaaacc gaaactctgg tagaagtttt
    15301 gcagatgcgc ctgcgccccg aggtagacag gcttatcagg ccagtgatgc gtgcaagtgt
    15361 cgagggtttt actcatcagt tgctgcccca ctttttcgcc acgcaacgct tcactgacaa
    15421 tcacccgacc aataacgacc ggctcaagat catcatcact tttcagaatc ctcgcatatg
    15481 ccaccagttc atcatttttc caaccgagaa tatggcgatt atcccccgtc aaatcatcac
    15541 catcgatatc ctgataaggg caattttgct cgacaacaaa caccgcgcaa cgcagttgta
    15601 ataaggcata aagctgggaa accgataatt cagagtgatg cagatcttgc cattcaatca
    15661 taatgctctc cttttagatg tatgccttat actagccgga atattttttg aaacgtgatg
    15721 aactcatgga attaattttt ttaggtactt cggccggtgt gccaacccgc acgcgcaatg
    15781 tcacggcaat attgctaaac ctgcaacatc cgacccagag cggactttgg ttgtttgact
    15841 gcggtgaagg cacccagcat cagctactgc ataccgcctt taaccctgga aaactggaca
    15901 agatttttat cagtcacctt catggcgatc atctttttgg tttacccggc ttgctgtgca
    15961 gtcgttctat gtcaggcatt atccaaccct taacgattta tggtccgcaa ggtatccgtg
    16021 aatttgtgga gaccgcgctg cggattagcg gctcatggac cgattatccg ctggaaattg
    16081 tcgaaattgg cgctggcgaa attctcgatg atggcctgcg caaagtgacc gcttatccaa
    16141 tggaacaccc actggaatgc tatggctatc gtattgaaga acatgataaa ccgggtgcat
    16201 taaatgccca ggcattaaaa gctgctggcg taccgcctag cccactgttt caggaattaa
    16261 aagcgggcaa aacaatcatg ctggaagatg gaaggcagat taacggcgca gattacttag
    16321 ctgctccagt gccaggtaaa gcgctcgcta ttttcggcga taccggcccc tgcgatgccg
    16381 cacttgacct ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg gatataacca
    16441 tggaagccaa agccaatagt cgcggtcata gctctacacg ccaggctgcg acactggccc
    16501 gtgaggctgg agtcggtaag ctaatcatta cccacgtcag ctcgcgctat gatgacaaag
    16561 gttgtcagca cctgttacgt gaatgcaggt caattttccc ggcgactgaa ctagcgaatg
    16621 atttcaccgt gtttaacgtt taactctgct tattattaaa aacagggcga aacttgccct
    16681 gttatcgcaa cccgcgctac atatatccac tcacacgggc atattgagcg atttttgata
    16741 tttattcatc tccttacgca ctatattcag agcaataagt tgcctctttt tatcttcatt
    16801 cagcacaacc tttgcaacta aaaggagcat atgatggtta cagttgtcag caattattgt
    16861 caattatctc aaacccaact cagtcagaca tttgcagaaa aatttactgt gaccgaggaa
    16921 ttactgcagt ctttaaaaaa aacagcgtta tccggagatg aagaaagcat tgagttactg
    16981 cataatattg cgttaggtta tgatgaattt gggaaaaaag ctgaagatat tctttaccat
    17041 attgttagaa acccaacaaa tgatacccta tcgatt
//
LOCUS       DAZKFZ010000088        17021 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000088 DAZKFZ010000000
VERSION     DAZKFZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            22..645
                     /gene="nfeR"
                     /locus_tag="ACHOQW_002631"
                     /gene_synonym="yqjI"
     CDS             22..645
                     /gene="nfeR"
                     /locus_tag="ACHOQW_002631"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP9573693.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(799..2319)
                     /gene="aer"
                     /locus_tag="ACHOQW_002632"
     CDS             complement(799..2319)
                     /gene="aer"
                     /locus_tag="ACHOQW_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP9573694.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2737..4116
                     /gene="ygjG"
                     /locus_tag="ACHOQW_002633"
     CDS             2737..4116
                     /gene="ygjG"
                     /locus_tag="ACHOQW_002633"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP9573695.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4158..4490)
                     /gene="ygjH"
                     /locus_tag="ACHOQW_002634"
     CDS             complement(4158..4490)
                     /gene="ygjH"
                     /locus_tag="ACHOQW_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP9573696.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            4709..5692
                     /gene="ebgR"
                     /locus_tag="ACHOQW_002635"
     CDS             4709..5692
                     /gene="ebgR"
                     /locus_tag="ACHOQW_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP9573697.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5876..8968
                     /gene="ebgA"
                     /locus_tag="ACHOQW_002636"
     CDS             5876..8968
                     /gene="ebgA"
                     /locus_tag="ACHOQW_002636"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP9573698.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            8965..9414
                     /gene="ebgC"
                     /locus_tag="ACHOQW_002637"
     CDS             8965..9414
                     /gene="ebgC"
                     /locus_tag="ACHOQW_002637"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP9573699.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9477..10910
                     /gene="ygjI"
                     /locus_tag="ACHOQW_002638"
     CDS             9477..10910
                     /gene="ygjI"
                     /locus_tag="ACHOQW_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9573700.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            11044..12114
                     /gene="ygjJ"
                     /locus_tag="ACHOQW_002639"
     CDS             11044..12114
                     /gene="ygjJ"
                     /locus_tag="ACHOQW_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP9573701.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            12131..14482
                     /gene="ygjK"
                     /locus_tag="ACHOQW_002640"
     CDS             12131..14482
                     /gene="ygjK"
                     /locus_tag="ACHOQW_002640"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9573702.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            15008..>17021
                     /gene="fadH"
                     /locus_tag="ACHOQW_002641"
     CDS             15008..>17021
                     /gene="fadH"
                     /locus_tag="ACHOQW_002641"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP9573703.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT         4082 a         4567 c         4478 g         3894 t
ORIGIN      
        1 attaagcaaa aggaggctga tatgagccat catcacgaag ggtgttgtaa acatgaaggc
       61 cagccacgcc atgagggctg ctgcaaaggt gagaagtcag aacacgagca ctgcggacac
      121 ggtcaccagc atgaacacgg tcaatgttgc ggtggtcgcc acggtcgcgg cggcggtcgt
      181 cggcaacgtt tctttggtca cggtgaattg cgtctggtga ttctggatat tctctcgcgc
      241 gatgacagcc acggttacga attgattaaa gcgattgaga atctaaccca agggaattac
      301 accccaagcc cgggcgtcat ctacccgacg ctggattttc tgcaggagca gtcgctgatt
      361 accatccgcg aagaggaagg tggtaagaag cagattgcgc tgaccgaaca aggcgcgcag
      421 tggctggaag aaaaccgcga acaggtggag atgattgaag aacgcatcaa agcgcgttgc
      481 gttggcgctg cgctgcgcca gaacccgcaa atgaagcggg cgctagataa ttttaaagcg
      541 gtgctgaatt tacgcgtcaa ccagagtgat atcactgatg cacaaataaa aaagatcatt
      601 gcggtgatcg accgcgccgc ttttgatatt acgcaactgg attaatcgcc gcatccgcca
      661 gtggcgcggt gcaattgccg gatgcgacgc ttgacgcacc ttatccggcc tacacccgct
      721 acacaccccg taggcctgat aagatgcgcc agcatcgcat caggcatcgt gtatcaaata
      781 ccggatccgg cataccgatt aatgcagcac cgtcaccgcg tcttccagtc ggctggcgcg
      841 gtgtttcacc atcgccgaca cctgcgcact ctcttccacc agctcggcat ttttctgggt
      901 gatgaggtta agctcatcca ctgcacgggt caggctggaa agaccatcgg cctgttccag
      961 cgttgaatgg ctaatctggg caatcaactg ggtgacgttt ttcacctgtg ccacaatatc
     1021 ttccatcgta cgtccggcgg cgtgtacctg ctgcgaaccg gattgcacct tatcagcact
     1081 ggcatcaatc agcttgcgaa tatcgttggc agcattggcg ctgcggctgg ctaaatgacg
     1141 cacttccccc gccaccaccg caaaaccttt gccctgttcg ccggcacgcg ccgcttccac
     1201 cgccgcattc agggccagaa tattggtctg aaacgcaata tcgttaatca gcgaagtaat
     1261 ggtgccaatg cgctgggtac tgtcggcgat atcgtccatt gtcttgatca ccgtggtcat
     1321 cgcctccccg ccctgcactg ccgcattact ggcggtgatt gacagtttat cggcagccga
     1381 cgccgtggca gagttctgtt tcaccgacgc cgccatttgg ttcatggtcg ccaccgtttg
     1441 ctgaacgtta tcaactgtct gctgggtatg ttcgttcagt tcatcggtgc ctttcgccag
     1501 cgtctcactg ccgtttctga cactggacac ctggcttgag acatcgttaa ttaaccaacg
     1561 gcacatcagg ccaagctgcc ctaccgcgcg taatgtcagc cccagctcat cgctgcgatt
     1621 cagatgctca acactattac gctctccggt cgccaccttc agtgcctgac gggcgacatt
     1681 ttctatcggg cggacaatct gccattcaaa acaggcgctt gccaacaata ccactaacgc
     1741 acagaggata tacgtcacca ccggggcagc aacaaaccaa agcatggccg ccagcaagat
     1801 aaacatcagg gtcatcactc cacgcgcccg ccagcgaagc ggtaatgaag gcagtttacc
     1861 cagccagcct ttacgcacca ccaggccttt atgaatacgc ttaccggtac gtccggcgtt
     1921 cagcgctttg tacagcggct ccaccgccgc gatctcttca tccgtcgccc gggtacgaat
     1981 cgacatatag ccactgattt ttccctcgcg caccatcggt accgcattgg cccgcaccca
     2041 ataatggtca ccatttttgc ggcgattttt cacgatgccg ctccagggct cccctttttt
     2101 cagggtgaac cacatatccg caaacgccgc ttttggcata tccgggtgac gcaccatgtt
     2161 gtgcggctgc ccttgtaact cttgcaaggt aaagccgctc acctgcacaa aagtgtcatt
     2221 agcatgagtg atatagcttt gcagatcggt agtggacatc agagtggtat cgtccgccag
     2281 cggggtattt tgctgggtga catacggatg agaagacatg ttcgcgtcct gtgcaggtta
     2341 tatggttgtt aacttcttgt cagagtttat gtcggccccg ctgcggttat ctttaaccga
     2401 ttaatttgat ttagatcgca atttgcgatt aaagcacaaa tctaattcgt tgatttaaaa
     2461 tactttcact ctgttactat acgaaaacgt taattatctt gcccaaaaat caggcagtta
     2521 atgccctgaa agcgtgcatt tgcgcagcaa ttatcaaatc catacccgac aaaaaccgtg
     2581 caaaataaca acagatgtta acaaatagca ttaaatattg cgcaaatgat aaccacattt
     2641 gtgtttatcc cgattttcgc gatcgcagcc ggagtggcgc aatccctgca atacttaaat
     2701 cggtatcatg tgatacgcga gcctccggag catattttga acaggttacc ttcgagcgca
     2761 tcggctttag cgtgcagcgc ccacgccctg aatctcattg aaaagcgaac gctggatcat
     2821 gaggagatga aagcacttaa ccgagaggtg attgaatact tcaaagagca tgtcaatccg
     2881 gggtttttag agtatcgcaa atctgttacc gccggcgggg attacggagc cgtagagtgg
     2941 caagcgggag gtttaaatac gcttgtcgac acccagggac aggagtttat cgactgcctg
     3001 ggaggttttg gaattttcaa cgtggggcac cgtaatccag ttgtggtttc cgccgtacag
     3061 aatcaacttg cgaaacaacc actgcacagc caggagctac tcgatccgtt acgggctatg
     3121 ctggcgaaaa cccttgctgc gctaacgcca ggtaaactga aatacagctt cttctgtaat
     3181 agcggcaccg agtccgttga agcggcgctg aagctggcga aggcttatca gtcgccgcgc
     3241 ggcaaattta cttttattgc caccagcggc gcgttccacg gtaaatcact tggcgcgctg
     3301 tcggccacgg cgaaatcgac cttccgcaaa ccgtttatgc cactgctgcc agggttccgt
     3361 catgtgccgt ttggcaatat cgaagccatg cgcacggctc ttaatgagtg caaaaaaacc
     3421 ggtgatgatg tggccgcggt gatcctcgaa ccgattcagg gcgaaggtgg cgtaattctg
     3481 ccgccgccgg gctatctcac cgccgtgcgt aagctgtgcg atgagttcgg cgcactgatg
     3541 atcctcgatg aagtacaaac gggcatgggg cgcacgggca agatgttcgc ctgcgaacat
     3601 gagaacgtac agccggacat cctgtgcctg gcaaaagccc tcggcggcgg tgtgatgccg
     3661 attggcgcaa ccatcgccac tgaagaggtg ttctcagttc tgttcgacaa cccgttcctg
     3721 cataccacca cctttggcgg caacccgctg gcctgtgcgg cggcgctggc gaccatcaat
     3781 gtgttgctgg agcagaactt accggctcag gctgagcaaa aaggcgatat gttgctggac
     3841 ggtttccgtc aactggcgcg ggaatatccc gatctggtac aggaagcgcg tggtaaaggg
     3901 atgttaatgg cgattgagtt tgttgataac gaaatcggct ataactttgc cagcgagatg
     3961 ttccgccagc gcgtactggt ggccggaacg ctcaataacg ccaaaacgat ccgcattgaa
     4021 ccgccactga cactgaccat tgaacagtgt gaactcgtga tcaaagcggc gcgtaaggcg
     4081 ctggcggcca tgcgagtaag tgtcgaagaa gcgtagtatc aaatcggatg gcaatacgac
     4141 gtcgccatcc gatcttttta tacgatacgt acgcccgcag gcatcatccg ctccggcgtt
     4201 aacagcacgc tttcactgcc gtcatcggtt tccgcacaca acagcatgca ttccgacgtt
     4261 tcaccacgca ttttcgcttt ttgcagattg cataagacca ccaccgtttt tcccatcaac
     4321 tcttcttcgc tgtagtacgg caccaggctg gtcacggttt gcagtgtttt ttcccccaca
     4381 tcaacctgta cgatgtacag cttgtcggcg ttttcatggc gtttcacttc cacaatcttt
     4441 ccgacgcgca tttccagacg tgcaaaatca gcgtaagcca cggtttccat tgcttcctcc
     4501 cttcgagtaa aattttacta aactatagca aaagtttttc tcaatcctgt aggctaaaaa
     4561 tggagaatgc aggcgtgatc acattcgtga gccgctgtgt taccgttaca gcgtcaaaga
     4621 aacgcgcttt atttactgaa aacaggtgac ccgataagca cttcctctac aatgggggcg
     4681 cacatcaggg aaagtaaaaa aggtaaacat ggcaacacta aaagacatcg caatcgaagc
     4741 tggcgtatcc ctggcgacag tatccagggt cttaaatgac gatccgacat tgaatgtgaa
     4801 agaagagacg aaacatcgca ttctcgagat cgccgaaaag ctggagtaca agaccagtag
     4861 cgcccgtaaa ctacagacag gcgcagtcaa ccaacaccat attctggcta tctacagcta
     4921 ccagcaggag ctggagatca acgatcctta ctatctggcg atccgccacg gcattgaaac
     4981 tcagtgcgaa aagctggcga tcgaactgac caactgttat gaacacagcg gattaccaga
     5041 cattaaaaac gtcaccggta ttttaattgt cggcaaaccc acgcccgccc tgcgcgccgc
     5101 tgccagcgcg ttgaccgaca atatctgttt tatcgacttt cacgaacccg gcagcggtta
     5161 cgatgcggtg gatatcgatc tggcacgcat cagtaaagaa atcatcgact tctatatcaa
     5221 ccagggcgtt aatcgtattg gttttattgg cggtgaagat gagcctggca aggcggatat
     5281 tcgtgaggtc gcctttgcgg aatatggccg actgaaacaa gtggtacgcg aagaggatat
     5341 ctggcgcggc ggtttttcca gttcgtcggg ttatgaactg gcaaaacaaa tgctggcgcg
     5401 ggaagactat ccgaaggcac tgtttgttgc ttccgattcc attgctatcg gcgtactgcg
     5461 ggcgattcat gaacgtggtc tgaacatccc acaggatatt tcgcttatca gcgttaacga
     5521 tatccccacc gcgcgattta cctttccgcc gctctccacc gtgcgcatcc attccgaaat
     5581 gatgggaagt cagggcgtaa acctggtgta tgaaaaagcc cgcgatggtc gcgcgctgcc
     5641 gctgttagtc ttcgttccca gcaaattaaa actgcgcggc acgacccgtt aaatcccctt
     5701 acacactgtc cggcaatcgt ttttgccggg cagtgctgcc gtttattttc gtgatccagt
     5761 taaagtaaat gcatttaccc gctacttttt agtaaaaatt ttactaaact ccccagcaat
     5821 tacacaaact accatcacca tgaatggttc cgatttctct ctaccgggag gccctatgaa
     5881 tcgctgggaa aacattcagc tcacccacga aaaccgactt gcgccgcgtg cgtacttttt
     5941 ttcatatgat tctgttgcgc aagcgcgtac ctttgcccgc gaaacgagca gcctgtttct
     6001 gcccttaagc ggtcagtgga atttccactt ttttgaccat ccgctgcaag tgccagaagc
     6061 cttcacctct gagttaatgg ctgactgggg gcatattacc gtccccgcca tgtggcaaat
     6121 ggaaggtcac ggcaaactgc aatataccga cgaaggtttt ccgttcccca tcgatgtgcc
     6181 gtttgttccc agcgataacc caaccggtgc ctatcaacgt attttcaccc tcagtgacgg
     6241 ctggcagggt aaacagacgc tgattaaatt tgacggcgtc gaaacctatt ttgaagtcta
     6301 cgttaacggt cagtatgtgg gtttcagcaa gggcagtcgc ctgaccgcag agtttgacat
     6361 cagcgcgatg gttaaaaccg gcgacaacct gttgtgtgtg cgcgtgatgc agtgggcgga
     6421 ctctacctac gtggaagacc aggatatgtg gtggtcagcg gggatcttcc gcgatgttta
     6481 tctggtcgga aaacacctaa cgcatattaa cgatttcact gtgcgtaccg actttgacga
     6541 agcctattgc gatgccacgc tttcctgcga agtggtgctg gaaaatctcg ccgcctcccc
     6601 tgtcgtcacg acgctggaat ataccctgtt tgatggcgaa cgcgtggtgc acagcagcgc
     6661 cattgatcat ttggcaattg aaaaactgac cagcgccagc tttgctttta ctgtcgaaca
     6721 gccgcagcaa tggtcagcag aatcccctta tctttaccat ctggtcatga cgctgaaaga
     6781 cgccaacggc aacgttctgg aagtggtgcc acaacgcgtt ggcttccgtg atatcaaagt
     6841 gcgcgacggt ctgttctgga tcaataaccg ttatgtgatg ctgcacggcg tcaaccgtca
     6901 cgacaacgat catcgcaaag gccgcgccgt tggaatggat cgcgtcgaga aagatctcca
     6961 gttgatgaag cagcacaaca tcaactccgt gcgtaccgct cactacccga acgatccgcg
     7021 tttttacgaa ctgtgtgata tctacggcct gtttgtgatg gcggaaaccg acgtcgaatc
     7081 gcacggcttt gctaatgtcg gcgatatcag ccgtattacc gacgatccgc agtgggaaaa
     7141 agtctacgtc gagcgcattg ttcgccatat tcacgcgcag aaaaaccatc cgtcgatcat
     7201 catctggtcg ctgggcaatg aatccggcta tggctgtaac atccgcgcga tgtaccacgc
     7261 agcgaaggcg ctggatgaca cgcgactggt gcattacgaa gaagatcgcg atgctgaagt
     7321 ggtcgatatt atttccacca tgtacacccg cgtgccgctg atgaatgagt ttggtgaata
     7381 cccgcatccg aagccgcgca tcatctgtga atatgctcat gcgatgggga acggaccggg
     7441 cgggctgacg gagtaccaga acgtcttcta taagcacgat tgtattcagg gacattatgt
     7501 ttgggagtgg tgcgaccacg ggatccaggc gcaggatgac aacggcaatg tctggtataa
     7561 attcggcggc gactacggcg actatcccaa caactataac ttctgtcttg atggtttgat
     7621 ctattccgat cagacgccgg gaccaggcct gaaagagtac aaacaggtta tcgcgccggt
     7681 aaaaatccac gcgctggatc tgactcgcgg cgagctgaaa gtcgaaaata aactgtggtt
     7741 taccacgctg gatgactaca ccctgcacgc agaggtgcgc gccgaaggtg aaacgctcgc
     7801 gacgcaacag attaaactgc gcgacgttgc gccgaacagc gaagccccct tgcagatcac
     7861 gctgccgcag ctggacgccc gcgaagcgtt cctcaacatt acggtgacca aagattcccg
     7921 cacccgctac agcgaagccg ggcattctat cgccacttat cagttcccgc tgaaggaaaa
     7981 caccgcgcag cctgtacctt tcgcaccgaa taacgcccgt ccgctgacgc tggaagacga
     8041 tcgtttgagc tgtactgttc gcggctacaa ctttgcgatc accttctcaa aaacgagtgg
     8101 taaaccgaca tcctggcagg taaatggcga atcgctgctg acccgcgagc caaagatcaa
     8161 cttcttcaag ccaatgatcg acaaccacaa gcaggagtac gaagggttgt ggcagccgaa
     8221 tcatttgcag atcatgcagg aacatctgcg cgactttgcc gtagaacaga gcgatggtga
     8281 agtgttgatc atcagccgca cggttatagc accgccggtg tttgacttcg ggatgcgctg
     8341 tacctacatc tggcgcatcg ctgcagatgg ccaggttaac gtggcgcttt ccggcgagcg
     8401 ttacggcgac tatccgcaca tcattccgtg catcggtttc accatgggga ttaacggcga
     8461 atacgatcag gtggcgtatt acggtcgtgg accgggcgaa aactacgccg acagccagca
     8521 ggctaacatc atcgatatct ggcgcagcac cgtcgatgcc atattcgaga actatccctt
     8581 cccgcagaac aacggcaacc gtcagcatgt ccgctggacg gcactgacta accgccacgg
     8641 caacggtctg ctggtggttc cgcagcgccc aattaacttc agcgcctggc actataccca
     8701 ggaaaacatc cacgctgccc agcactgtaa cgagctgcag cgcagtgatg acatcaccct
     8761 gaatctcgac caccagctgc ttggcctcgg ctccaattcc tggggcagcg aggtgctgga
     8821 ctcctggcgc gtctggttcc gtgacttcag ctacggcttt acgttgctgc cggtttctgg
     8881 cggagaagct accgcgcaaa gcctggcgtc gtatgagttc ggcgcagggt tcttttccac
     8941 aaatttgcac agcgagaata agcaatgagg atcatcgata acttagaaca gttccgccag
     9001 atttacgcct ctggtaagaa gtggcaacgc tgcgttgaag cgattgaaaa tatcgacaac
     9061 attcagcctg gcgtcgccca ctccatcggt gactcattga cttaccgcgt ggagacagac
     9121 tccgcgaccg atgcgctatt taccgggcat cgacgctatt ttgaagtgca ttactacctg
     9181 caagggcagc aaaaaattga atatgcgccg aaagagacat tacaggtcgt ggaatattat
     9241 cgtgatgaaa ctgaccgtga atatttaaaa ggctgcggag aaaccgttga ggtcgacgaa
     9301 gggcaaatcg ttatttgcga tatccatgaa gcatatcggt ttatctgcaa taacgcggtc
     9361 aaaaaagtag ttctcaaagt caccatcgaa gatggttatt tccataacaa ataacaacta
     9421 cggcggcaaa aggagtttgc cgccaccgct accctactca ttttcggaga tgtgttatgt
     9481 ctgataccaa acgtaataca atcggcaaat tcggcttgct ctcgctgact tttgccgccg
     9541 tttacagctt taacaacgtt atcaataata atattgagct tggactggcc tcggcaccga
     9601 tgtttttcct cgcgacgatt ttttatttta ttcccttctg tctgatcatc gcagaatttg
     9661 tttcgttaaa taaaaactca gaagccggtg tctacgcgtg ggtaaaaagt tcgctgggcg
     9721 gacgttgggc atttattact gcctatacct actggttcgt aaacctgttc tttttcacct
     9781 cgctgttgcc gcgcgttatt gcttatgctt cgtatgcctt cctcggctat gaatatatta
     9841 tgacgccggt tgccaccacc attatcagta tggtgctgtt cgccttctcc acctgggttt
     9901 ccaccaacgg ggcgaaaatg ttggggccaa ttacctccgt cacttcaacg ctgatgctgc
     9961 tgttaacgct ctcctacatt ttactggcag gtacggcgct ggttggcggc gtacagcctg
    10021 ctgaccccat caccgttgac gcgatgatcc cgaacttcaa ctgggcgttc ctcggcgtta
    10081 ccacctggat ctttatggcc gcaggtggcg cggagtccgt cgctgtgtac gttaacgacg
    10141 tcaaaggcgg ttcgaaatcg ttcgttaaag tgatcatcct cgccgggatt tttatcggtg
    10201 tactatattc cgtctcctcg gtgctgatta acgtcttcgt cagcagcaaa gagttgaaat
    10261 ttactggcgg atcggtgcag gtattccacg gcatggcggc gtattttggt ctgccggaag
    10321 cgttgatgaa tcgctttgtc ggtctggtgt cctttaccgc aatgttcggt tccctgctga
    10381 tgtggaccgc aacgccggtg aaaattttct tctccgaaat cccggaaggc atctttggta
    10441 agaaaaccgt cgaacttaac gaaaacggcg ttccggcgcg cgcagcgtgg atccagttcc
    10501 tgatcgtcat cccgctgatg attatcccga tgctcggttc caatactgtg caggatctga
    10561 tgaatactat tattaatatg accgccgcag cgtccatgct tccgccgtta ttcatcatgc
    10621 tggcttacct gaatttacgc gccaaattag atcacctgcc acgcgatttc cgtatgggct
    10681 cccgccgcac cggtattatc gttgtttcaa tgctgattgc gatatttgcc gtagggtttg
    10741 tcgcttcgac attcccgact ggcgcgaata ttctgaccat cattttttat aacgtcggcg
    10801 gtattgttat attccttggc tttgcgtggt ggaaatacag taaatatata aagggattaa
    10861 cggctgaaga gcgccatatt gaagcgacgc cagccagcaa tgttgattaa acagactaat
    10921 aaaaaatgag caggcatttt ccctcttcct ggatgaggag agggaaaata gtttctgcct
    10981 ttatattttt ataattacaa cgataaaagg ctgtactttt tctttagctc atggattaac
    11041 acaatgaaat taatcactgc accatgcaga gcattacttg ctctgccgtt ttgctacgcc
    11101 ttttctgcgg caggagaaga agcacgtccg gcagaacatg acgacacaaa aacccccgca
    11161 attacctcga catcttctcc ttcatttcgt ttttacggcg aattaggggt tggtggatat
    11221 atggatttag agggtgagaa taaacataaa tacagcgacg gcacctatat tgaaggtggc
    11281 ctggagatga agtacggctc ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag
    11341 gccgaccacg acggcaatgc ctgggtgcct gaccatagct ggggcggttt cgaaggtggg
    11401 attaaccgtt tctatggcgg ttatcgaacc aatgatggca ccgaaatcat gctcagtctg
    11461 cgtcaggatt cctcgctgga tgacctgcaa tggtggggcg acttcacacc cgatctgggc
    11521 tacgtcattc ccaatacccg cgacattatg accgcgctga aggtgcagaa cttaagcggc
    11581 aactttcgtt atagcgtcac cgccacacct gccggacatc atgacgaaag caaagcatgg
    11641 ctacattttg gcaagtacga tcgctacgac gacaaataca cctatccggc aatgatgaac
    11701 ggttacatcc agtatgacct tgccgaaggc atcacctgga tgaatggtct ggaaattacc
    11761 gacggcacag gacaactcta tctcacggga ctgcttaccc ctaactttgc cgctcgcgcc
    11821 tggcaccata ccggacgtgc cgacgggctg gacgtaccgg gaagtgaaag cgggatgatg
    11881 gtgagcgcca tgtatgaagc gttaaagggc gtttatctct ccaccgctta cacctacgcc
    11941 aaacatcgcc ctgaccacgc tgacgatgaa accacctctt tcatgcagtt tggtatctgg
    12001 tacgaatatg gcggcggacg tttcgcaacg gcttttgata gccgcttcta catgaaaaat
    12061 gcctctaacg atcccagcga ccaaatcttc ctgatgcaat atttctactg gtaataagga
    12121 ctgtaatttc atgaaaatca aaactatttt aacgccagta acctgcgctc tgctgataag
    12181 tttttccgcc catgccacta acgccgacaa ttataaaaac gtgattaacc gtactggtgc
    12241 gccgcagtac atgaaggatt acgattacga cgatcaccag cgttttaatc cgtttttcga
    12301 tctcggagcc tggcatggtc atctgttgcc agacggcccg aacaccatgg gcggctttcc
    12361 gggcgttgcg ctgctgacgg aagaatacat caactttatg gccagcaatt tcgaccgcct
    12421 gaccgtctgg caggacggca agaaagtcaa cttcacgctg gaggcataca atattcccgg
    12481 cgcgctggtg caaaaactga cagcaaaaga tgtgcaggtc gaaatgactc tgcgcttcgc
    12541 cacgccgcgc acgtcactac tggaaactaa aatcatcagc aataaaccgc tggatctggt
    12601 gtgggatggc gaactgctgg aaaaactgga agcgaaagaa gggaaaccgc tttccgataa
    12661 aaccattgct ggcgaatacc ctgactacca gcgcaaaatc agcgccaccc gtgatggcct
    12721 gaaagtcacc tttggcaaag tgcgcgccac ctgggatctg ctgacctccg gtgaatcaga
    12781 atatcaggtg cataaatccc tgccggtgca gactgaaatc aacggcaatc gctttaccag
    12841 taaggcgcat atcaacggtt cgacgacgct ctataccacc tattcccatc tgctgacctc
    12901 tcaggaagtt agcaaagagc aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta
    12961 tctcaccgcc tcgcagcaac gctgggaaga gtatctgaag aaagggttaa ccaatccgga
    13021 tgcgacgccg gaacagacac gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg
    13081 gcgctcgcct gggggtgcgg tgaaatataa caccgtcacg ccgtcggtga ccgggcgctg
    13141 gttctccggc aatcagacct ggccgtggga tacctggaag caggcgtttg cgatggcgca
    13201 tttcaatccg gacatcgcca aagagaatat ccgcgcggtc ttctcctggc agatccagcc
    13261 tggcgacagc gtgcgtccgc aggatgtggg ctttgtcccc gacctgatag cgtggaatct
    13321 tagccccgag cgtggtggcg atggtggcaa ctggaacgaa cgaaatacca aacccagcct
    13381 tgccgcctgg tcggtgatgg aagtgtacaa cgtcacccag gataaaacct gggtggcaga
    13441 gatgtacccg aaactggtgg cctatcacga ctggtggtta cgtaaccgcg atcacaacgg
    13501 caacggcgtg ccggaatatg gcgcgacccg cgacaaagcc cacaacactg agagcggcga
    13561 gatgctgttt acggtgaaga aaggcaacaa agaagagacg cagtctgggc tgaacaacta
    13621 cgcccgcgtg gtggagaaag gccagtacga cagtctggaa attccggcac aggttgctgc
    13681 atcgtgggaa tcggggcgtg atgatgccgc cgtctttggg tttatcgaca aagaacagct
    13741 ggataaatat gtcgcaagcg gcggcaaacg tagcgactgg acagtgaaat tcgccgaaaa
    13801 ccgcagtcag gacggaacgt tgctggggta ctcgctattg caggagtcgg tggatcaggc
    13861 cagctatatg tacagcgata accattatct ggcggagatg gcgacgattc tcggtaaacc
    13921 ggaagaggct aagcgctatc gccagttggc acagcagctc gcggactaca tcaacacctg
    13981 tatgttcgac ccggctacgc agtacttcta tgacgtgcgt attgaagata aaccgctggc
    14041 gaacggctgc gcgggcaaac cgattgttga gcgcggtaaa gggccggaag gctggtcgcc
    14101 gctgtttaac ggtgcggcaa cgcaggccaa tgccgacgcg gtggtgaagg tgatgctcga
    14161 tcctaaagag ttcaatacct ttgttccgct gggaacggcg gcgttaacca atccggcctt
    14221 tggcgctgat atctactggc gcgggcgcgt atgggtggat cagttctggt ttggtctgaa
    14281 agggatggag cgttacggtt atcgcgatga tgccctgaaa ctggcggata cgttcttccg
    14341 gcacgccaaa gggttaaccg ccgatggccc aattcaggaa aattacaacc cgctgacagg
    14401 cgcacagcaa ggcgcaccaa atttctcctg gagtgccgca catttttata tgttgtataa
    14461 cgattttttc cgtaagcaat aatttgcaac ggctggcgga tgcggcgtga acgccttatc
    14521 cgccctacat attcgttccc gtaggctgga taagacgcga caagcgtcgc atccggcatc
    14581 tgcacctcgc caccggcgga tgctgcgtga acgccttatc cgccctacat gttcgttccc
    14641 gtaggctgga taagacgcga caagcgtcgc atccggcatc tgcacctcgc cactggcgga
    14701 tgcggcgtga acgccttatc cgccctacat gttcgttccc gtaggctgga taagacccga
    14761 caagcgtcgc atccggcgtc tgcaccgcgc cactggcgga tgcggcgtga acgccttatc
    14821 cgccctacat gtgcaatccc ccaggtcgga tagggcccgg caagcgtcgc atccggcgtt
    14881 accaccgggc gtattctttt tgaatcccat cacaaacccc gcactcccct tttccctttt
    14941 ctccggcgac ggctaaatta gaactcatcc gaccacataa caattatttt acatactgga
    15001 caattttatg agctacccgt cgctgttcgc cccgctggat ttaggtttta ccacgttaaa
    15061 aaaccgcgtg ttgatgggtt caatgcacac cgggctggag gaatacccgg acggtgctga
    15121 acggctggca gcgttttatg ccgaacgcgc ccgtcacggc gtggcgctga ttgttagcgg
    15181 cggcattgca ccagatttaa caggcgttgg catggaaggc ggcgcaatgc tcaatgacgc
    15241 cagccagatc ccacaccatc gcaccattac cgaagcggta catcaggaag gcggcaaaat
    15301 agccctgcaa attttgcata ccgggcgcta cagctaccaa ccgcatctgg tcgcaccgtc
    15361 cgcattgcag gcccccatca accgtttcgt tcctcatgaa ctcacccatg aagagatcct
    15421 gcaactgatc gacgatttcg cccgctgcgc gcaactggca cgggaggcag gatacgacgg
    15481 tgtagaggta atgggttccg aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa
    15541 tcagcgcagt gaccagtggg gcggcgatta ccacaaccgg atgcggtttg ccgttgaagt
    15601 ggtgagtgcg gtacgcgaac gcgttggcaa cgacttcatt attatctacc gactgtcgat
    15661 gctcgacctg gtcgaaggcg gcgggacttt tgccgaaacg gtagagctgg cgcaggccat
    15721 tgaagcggcg ggcgcgacca ttatcaacac cggcattggc tggcatgaag cgcgtattcc
    15781 gaccatcgcc acgcccgtgc cacgcggcgc atttagctgg gtcacgcgca aactgaaagg
    15841 tcacgtctcg ctgccgctgg tgaccaccaa ccggattaac gatccgcagg ttgccgacga
    15901 tattctctcg cgcggcgatg ccgatatggt atcgatggcg cgaccgtttc ttgcggatgc
    15961 ggaactgctg tcaaaagcgc aatcagggcg agccgatgag atcaacacct gcattggctg
    16021 caaccaggcg tgtctcgatc agatcttcgt tggcaaggtc acctcgtgtc tggtgaatcc
    16081 tcgcgcctgc catgaaacca aaatgccgat ccttcccgcc atgcagaaaa aaaatctggc
    16141 ggtggtcggt gcgggacctg ctgggctggc gtttgccatt aacgcggcgg cgcgtggtca
    16201 tcaggtaacg ttgtttgacg cgcacagtga gatcggcggg cagtttaata tcgccaaaca
    16261 gatccccggc aaagaggagt tttacgaaac gctgcgctat taccgccgga tgatcgaagt
    16321 gacgggcgtg acgctaaaac tcaatcacac cgtgacggcg gatcagttac aggcgttcga
    16381 tgaaacgatc ctcgccagtg ggatcgtgcc gcgcactccg cccatcgacg ggatcgatca
    16441 tccgaaggta ttgagttatc tcgatgtact gcgcgacaaa gcgccggtgg gcaacaaagt
    16501 tgccatcatc ggttgtggcg ggattggttt tgatacggcg atgtatttaa gtcagccggg
    16561 cgaatccacc agccagaata tcgccgggtt ctgtaatgaa tgggggatcg acagcagcct
    16621 acaacaggct ggtggcttaa gcccgcaggg aatgcagatc ccgcgtagcc cacggcagat
    16681 tgtgatgctc cagcgcaaag ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg
    16741 gatccatcgc accaccctgc tctcgcgggg tgtgaaaatg atcccaggcg taagttatca
    16801 gaagattgac gatgacgggc tgcatgtggt gatcaacggc gaaacgcagg tattagcagt
    16861 ggacaatgtg gtgatctgcg cagggcaaga gccaaaccgc gcattggcgc agccgctgat
    16921 tgatagcggg aaaacggtgc atttaattgg cggctgcgat gtggctatgg agctggacgc
    16981 acgacgggca attgcccagg gaacacggct ggcgctggag a
//
LOCUS       DAZKFZ010000089        16980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000089 DAZKFZ010000000
VERSION     DAZKFZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(38..616)
                     /gene="mocA"
                     /locus_tag="ACHOQW_002642"
     CDS             complement(38..616)
                     /gene="mocA"
                     /locus_tag="ACHOQW_002642"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP9573704.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            719..1489
                     /gene="yqeC"
                     /locus_tag="ACHOQW_002643"
     CDS             719..1489
                     /gene="yqeC"
                     /locus_tag="ACHOQW_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP9573705.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1537..3162
                     /gene="yqeB"
                     /locus_tag="ACHOQW_002644"
     CDS             1537..3162
                     /gene="yqeB"
                     /locus_tag="ACHOQW_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP9573706.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3203..4135)
                     /gene="arcC"
                     /locus_tag="ACHOQW_002645"
     CDS             complement(3203..4135)
                     /gene="arcC"
                     /locus_tag="ACHOQW_002645"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9573707.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4183..5568)
                     /gene="hyuA"
                     /locus_tag="ACHOQW_002646"
     CDS             complement(4183..5568)
                     /gene="hyuA"
                     /locus_tag="ACHOQW_002646"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP9573708.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5621..6832)
                     /gene="ygeY"
                     /locus_tag="ACHOQW_002647"
     CDS             complement(5621..6832)
                     /gene="ygeY"
                     /locus_tag="ACHOQW_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP9573709.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6890..8086)
                     /gene="dpaL"
                     /locus_tag="ACHOQW_002648"
     CDS             complement(6890..8086)
                     /gene="dpaL"
                     /locus_tag="ACHOQW_002648"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP9573710.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8144..9331)
                     /gene="ygeW"
                     /locus_tag="ACHOQW_002649"
     CDS             complement(8144..9331)
                     /gene="ygeW"
                     /locus_tag="ACHOQW_002649"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP9573711.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9810..11588
                     /gene="ygeV"
                     /locus_tag="ACHOQW_002650"
     CDS             9810..11588
                     /gene="ygeV"
                     /locus_tag="ACHOQW_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9573712.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLKT"
     gene            complement(11628..12107)
                     /gene="xdhC"
                     /locus_tag="ACHOQW_002651"
     CDS             complement(11628..12107)
                     /gene="xdhC"
                     /locus_tag="ACHOQW_002651"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP9573713.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12104..12982)
                     /gene="xdhB"
                     /locus_tag="ACHOQW_002652"
     CDS             complement(12104..12982)
                     /gene="xdhB"
                     /locus_tag="ACHOQW_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP9573714.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(12993..15290)
                     /gene="xdhA"
                     /locus_tag="ACHOQW_002653"
     CDS             complement(12993..15290)
                     /gene="xdhA"
                     /locus_tag="ACHOQW_002653"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP9573715.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            15706..16461
                     /gene="actS"
                     /locus_tag="ACHOQW_002654"
     CDS             15706..16461
                     /gene="actS"
                     /locus_tag="ACHOQW_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP9573716.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            16540..16613
                     /locus_tag="ACHOQW_002655"
     tRNA            16540..16613
                     /locus_tag="ACHOQW_002655"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16572..16574,aa:Gly,seq:ccc)
BASE COUNT         4100 a         4089 c         4371 g         4420 t
ORIGIN      
        1 gattaaatat ttgaacgcaa tcgtttaact acgtttttta aattttagta tacctttttt
       61 gcgctgtaat aaagtcgtcc ggagtatcaa tatctaaaat tatttctgca ttttctactt
      121 cgacggaata atgagctccc ataaggagtg cctgacgcat attggtgata ttggggcgct
      181 tgattgcctg catcaggcat ggttttgaca ctaaaatcgg atggccgggg atgccattat
      241 ggagtggcag tattgcgcca tcatttcgta acgaccagat ttttctgaaa atatcgatgg
      301 tgagggttgg catgtcaccg tgggtgagaa aacaatgttc tgtttgcact gcgggtactg
      361 cggcctttac tgatgttagt aaaccctgcg cataatctgg gttgtggata atagtgatat
      421 tgctctggtt cgcataacgt tcgtgcagtt cattaccacg atagccggtg actaaaataa
      481 ttcggctaca aaactgcaac gcatttttga tacttgtatc aagaattgtc ccctgttgcc
      541 agggtaacat cattttccat tgccccattc ttgatgataa tccggcagcg gtaattatac
      601 agtcgatggc tgacatagtg gttccattat ttataggggc taataacgcc tgataattgc
      661 gttacttcct gatggattaa gttggggata atttatccca gagaggtcat aaagactcgt
      721 gaaaagtata gttgacccat cggcattatt cattgattta ggtgcgcaga aacgtcccac
      781 tgtgatttct gttgttggtg ctggggggaa aaccagcttg cttttttggc tggcagaact
      841 gctccaggct agcggcaggc gtgtgttaat cacgacgaca acacatatgt ttatgccaac
      901 gtctcactgg cccgtggttt tctgtcgtga tcccgccatg cttcctcatg cgtctcttac
      961 atcccccatt tcattttgtt ttcacagctg gaaagcgaac ctgggaaaag tgcagggatt
     1021 tacgccagaa gcgattgatg cactggtgca acgaccagag tgtgacgtaa ttctcattga
     1081 agcagatggc tcgcgtgcaa tgccgttaaa agcgcctgat gagcacgaac cttgcatacc
     1141 caaaagcagt tgctgcgtga ttgctgtgat gggaggacat attttgggcg cgaaagtgag
     1201 cacggaaaat gtccatcgct ggtcgcagtt tgctgatatt accgggttaa cacctgatgc
     1261 aaccttgcaa ctgagcgatc tcgttgcgct ggttcgccac cctcaggggg cgtttaaaaa
     1321 cgtaccgcaa ggttgtcggc gagtctggtt cattaaccgt ttttctcaat gtgagaatgc
     1381 gattgcgcaa agcgagctcc ttcaaccgct gcaacaacac gacgtagagg caatctggct
     1441 gggcgatata caagaacatc ctgcaatcgc gcgcagattt gtgaattagc ggcccgggta
     1501 ttccgggtta ctgaaaggat atccattgag gttggtatga atattttcac agaggctgca
     1561 aaactcgaag agcaaaattg tccgtttgct atggcgcaaa ttattgatag ccgaggctcg
     1621 actccccgcc attctgcaca aatgttagtg cgcgccgatg gctctatcgt cggtacaatt
     1681 ggtggcggaa tggttgagcg gaaggtgatt gaagagtcgc ttcaggcatt gcaggaacgt
     1741 aagccgcgat tattccatgg acgtatggct cgtaacggtg cggatgctgt cgggtcagat
     1801 tgtggaggtg cgatgtcagt gtttatcagc gtccatggta tgcgtccacg tctggtgttg
     1861 attggcgcgg ggcatgtcaa ccgggcgata gcccagagtg cggcgctatt aggatttgat
     1921 attgccgttg ccgatattta tcgcgaaagc ctcaatcctg aactattccc tccatcaacc
     1981 acgcttctcc atgctgagtc gtttggtgcg gcagtggaag cactggatat tcgccctgat
     2041 aattttgtcc tgattgccac gaataatcag gatcgtgaag ccctcgacaa actcattgaa
     2101 cagcccattg catggttggg gttgctggca agtcgtcgca aggttcagct tttcctgcgt
     2161 caattgcgtg agaaaggcgt ggctgaagaa catattgccc gtttacatgc gcccgttggt
     2221 tacaacatag gtgcggaaac gccgcaggag atcgccatca gcgtgctggc agaaatatta
     2281 caggtgaaaa ataacgcgcc gggtgggctg atgatgaaac cttctcatcc ttccggacac
     2341 cagctggtgg tgattcgcgg tgcgggagat atcgccagtg gtgtggcgct acgtctgtat
     2401 catgcgggtt ttaaagtgat catgctggaa gtggaaaaac cgacagtgat tcgttgtacc
     2461 gtggcgtttg cccaggccgt gttcgatggc gaaatgacgg tcgaaggcgt cactgctcgc
     2521 ctggcaacca gctctgcgga agcgatgaaa cttaccgaac gcggattcat ccctgtgatg
     2581 gtagatcccg cctgttcatt gcttgatgaa ctgaaaccgc tttgcgtggt ggacgctatt
     2641 ctggcgaaac agaatttggg aacacgggca gatatggcac cagtaacaat cgcgcttggg
     2701 ccgggctttg ctgcagggaa ggattgtcat gcggtaattg aaacaaatcg cgggcactgg
     2761 ctcggtcagg tgatttactg tggttgtgcg caggagaata ccggtgttcc tggcaatatt
     2821 atggggcata ccacccgacg ggtgatccgt gctcctgctg caggcattat gcgatccaac
     2881 gtgaaattag gcgatctggt gaaagagggc gatgtgattg cctggattgg tgagcatgaa
     2941 attaaagcac cgttgacggg gatggtgcgt ggcttgttga acgacggcct ggcagtggtt
     3001 ggtggtttta aaattggtga tatcgatcct cgtggtgaaa ccgctgattt caccagcgtt
     3061 tctgataaag cccgggcgat tggcggcggc gtacttgagg cgttaatgat gttgatgcat
     3121 cagggcgtga aagcgacaaa agaagtgctg gaagtggctt aaatgaaaat ggccggatgg
     3181 tattacccac ccggccttaa aattactgac aaatcaccgt tccggttttg ccttcaatcc
     3241 cttctttcgc tttgctcaat acggtaatca gtgcttcacg acctgcgcga gagcgagcaa
     3301 acgaggcggc agcttccact ttaggcagca tagaaccttt cgcaaaatga ccttcttcga
     3361 taaagcgttc agcatcactt aatgacaggc gatcgagcca ctgttcattc tctttaccga
     3421 agttaatggc cactttttct accgcagtca gaatgatcag catatcggca tcaatcattt
     3481 ctgctaaacg ggcgctggcc cagtctttat cgataaccgc gctggcacca cgcagatggt
     3541 taccttcacg aataacagga ataccaccac cgccaacggt gatcaccacc tggcctgctt
     3601 ctaccagcgc ttttaccgtc tctttttcaa tgatatcaac cggttttggc gaggcaacaa
     3661 cacggcgata gccgcgacca gcatcttctt tcagagtgta accctgtttt gtcagttgct
     3721 ctgcttcttg ctcggtaaag aacgaaccga tcggcttggt cgggttgagg aatgccggat
     3781 cgttagcgtc gacttcgacc tgagtaacca gcgttactac aggtttatta atgccccgag
     3841 aaagcagttc ttctcttaag gcgttttgta gatcgtaacc aatataaccc tggcttaagg
     3901 caacacagac agacatcggc agcattggcg aatgcgcttc agtttttgcg gcagcttcaa
     3961 aggcctggtt aatcatgccc acctgcggcc cgttaccatg agtgacgata acttcatgtc
     4021 cctgggcaat taaatcaaca attgcctgag aagtaatttt taccgctttc atttgtccgg
     4081 ccaggtcatc gcccagcgca ttcccgccca gggcgagaac aattttctta ctcatttttc
     4141 ctcactcatc attcttgatt gtgtatagat ccctggcagt ggttagagca cgggagggac
     4201 aaacggtttg cgtcgcagga aacgacctcg cccggctttg cctgtaaagg tgccgtcaca
     4261 gaaaatcgtt tcaccacggg ataaggttct gacaatcgcg ccctgacagg taaaaccctc
     4321 ccatggcgag tagtcggcgt tgtcgtggag atggcgatgc tgaatttgtt ggctctgacg
     4381 tgggtcgata atcaccacgt cgccatcgga accgggcgct aataatcctt tttgcggcca
     4441 caggccaaac agcctggcgg gcattgcgct ggttaattca acaaagcgtt ccggtgttat
     4501 acgtcccgtc atcacgccac tggaaaacag taactgcatg cggttttcca caccgggtaa
     4561 gccatttggg cagcgactga aatcgccttt agaaatttgc aggcgttgag ccatcgagaa
     4621 ggtgcagtga tcggtcgcca ccacgtcaat cgcaccatcg ctgatgccac accacagttt
     4681 gtcctgctcg cgtacgttac gcagcggtgg gctaagaatg aacttcatgc cgtcttctgt
     4741 atcgtaactg cgttcgtcca acaggagata ttgtgggcag gtttcaaccc agactggctg
     4801 gtgattcgca cgggcaagac gcagataatc cagacctaag ccgttagaca ggtgcacgat
     4861 atagagcggg gcgttaccgg caatttgtgc caggttaatc atgcgggcga tggcttccgc
     4921 ttcgcattcc agagggcgac tcaaggcatg atagcgcggc gcggttaacc ctgcggcgat
     4981 aaattccgcc cgcttgctgg cgatagccgc atcattttcc gggtgcacgg tggtcagcgc
     5041 gccggattca tgcagacggc gtaatgcctg caaaacctcg tcatcgttga gtttgtattg
     5101 ataggttaaa tagagtttaa aactgctcag cccttcctcg accatcatcg ggatttcgtc
     5161 gaggatggcg tgattaatgt gctggatcac accgtgaaag ctgtaatcga tgaccgcttt
     5221 atgggcggca taaccacgat aaacctccag ttgatggcgt aaccgacagc cgtttgggcc
     5281 aaatcccata tggtcaataa tggttgttgt accgccacac gcagctgcgc gggtaccggt
     5341 aaaaaaatca tcacaactgc gcgcgatgcc gacatcaata ttgaaatgcg tatggacatc
     5401 cacgccgcca gggaaaacgt aacagccagt ggcatcaatt tcttcatacg ggagctgcgg
     5461 cgaaatattg ttgcccaact ggcgaacaat cccgctttca atcagcaaat cctgtttggc
     5521 ttgtccatct gcgttaacga cagtgccgtt tttgatcaat acgcgcatag caaactccaa
     5581 aaaccctccg gcgaaccgga ggggaataag tgaagagtaa ttattcggtt gccagccagc
     5641 ttaacgggat tgcagcgtac atcgcggcac aggtcaccag gtgagatttc caggtttttt
     5701 cgttaggtgc atgcgcttca ggttctttac ccgggccaaa gccgatcacc ggaatgccgt
     5761 gacggcccat gatagatacg ccgttagttg agaaggtcca cttatcaaca accggcgctt
     5821 tgccaaacag cccttcgtag gcattcacca gtgctttaac ggtgaagtga tcttcttcca
     5881 ctttccaggt cgggaagtag cattcggttg ggtaaaccag gccagtccag gacggacggt
     5941 cgtagttgta catagaaaca accgcgttag ctttctgtac tgcaggcagg gcgcggattt
     6001 cgtccagcgc gccttcccag gtttcgcccc aggtcagacg gcggtcaata gagacggcgc
     6061 agctatctgc tacagcgcaa cggcttgggg atgtgaagaa gatttcagaa acggtgaggg
     6121 tgcctttgcc gaggaattcg tcataaccca gacgttggga gagttcttgt aattcgccaa
     6181 gaatcggacc cattttgaaa atggcgttgt caccgcgttc tggcgcagaa ccgtggcagc
     6241 taacaccctg aacatcaata cgaatttcca tacgaccgcg ctgaccacgg tatacctggc
     6301 agtcggttgg ttcggtactg accacaaatt ccgggcgaat gccggattgt tcaataatgt
     6361 actgccagca cagaccgtcg cagtcttctt cctgcacagt accggtaacc agcagggtat
     6421 attcatcttc cagaccgagg tctttaatga ttttaccggc ataaaccata gatgccatgc
     6481 cgccttcctg gtcggaagta ccgcgaccac cgatcagctc atcagtttcc atgccttcgt
     6541 acggatcgaa gtcccagttt ttgatgttgc caatgccgac ggtatcgata tgagcgtcca
     6601 ttgccaccag acgcgggccg tggccgatat aaccgagaac gttacccatc gggtcgattt
     6661 caactttatc gaagccgact ttttccatct cttctttaat acgatgtact acccgtttct
     6721 cgtcgcagct ttcactggga atagcaacca tgtcgcgcag gaagcgagtc atatccgcct
     6781 ggtaatcttt tgctttttca agaatcagtt tgaatggaat attcttagcc attatttttc
     6841 tccagatttt ttactccccg ttttgcaggg agcagttcca atagggtgat taaggtgcta
     6901 cagcgtgttt gccttcccag acaacttcgc ggtagtgctt cacgtcggtg tcgccttcag
     6961 tgctgataac cagcactacg gcatctttgt tcagcgccag tttttccatc aggctttgac
     7021 gttgcgggtg ataatgaacc gctgcgagaa cgcccaaacc gacagcgccg gattcaccgg
     7081 agatgatgcg cgggtcgttg ccgtacggat tacccagcac gcgcatacct aatgcggcaa
     7141 cgctgtcctg gcaggagatg aattgggtgg cgcagttacg taggatttcc cagcccagcg
     7201 ggttaggttc gccacaggcc aggcctgcca tgatggtggc catatcaccg ccaacgttga
     7261 cgatgtcgcc tttgacgccg gagcgataaa tacagtcagc tttgtcaggt tcaacaataa
     7321 tgctgtgcag attttgcggg ctatagacgt cgaccagata acccagcaca ccaccggcca
     7381 ttgctccgac accggcttgc agcagaacgt gcgtcggggt tacgcccatt tcacgcattt
     7441 gctcgacggc ttcatctgcc agggttgcgt agccttgcat gatccaggtt gggattttgg
     7501 tgtaaccttc ccatgccgtg tcctgtacca cttcccagcc gtgctgctgc gcgtgttgca
     7561 tggtcaggcg aacggtatcg tcatagttca tatctgtgac gatgcactcg gcaccgaggt
     7621 tcagaatggc gtcaacgcgt tcctgagcag aacctttcgg catgtaaatc accgcattct
     7681 gtccgagttg ctgcgctgcc cacgccacac cgcgcccgtg gttgccgtcg gtggtggtcg
     7741 cgaaagtcat tttttcgccg atggcatttt tcaggtgctc aaatgacagc gtttcgatat
     7801 caagatgata tttttcacac aataattgag cgatagcgta cgcaccgccg agcattttga
     7861 aggcgttcag accgaatcgt tttgattcgt ctttgacaag aattttcttc accccaaaaa
     7921 ggtttgcgag atcgtccagc gcacaaagcg gtgttgggcg ataaccagct attttctggt
     7981 ggaactggcg cgccagtttg gcctggcttt gcgaaaagag cggtgatgtt tcgccgttga
     8041 aaaatttgtt atcggcgata tcaatcttca atgagaaaac ggacatatcc tatccttttg
     8101 gcctctggaa atagagggcc gaccagctga tcggcccgag gggttatttc acgcgttctt
     8161 gcgcttcttt cagcagttgc tcgagcagtg cacctggttt ggcgtattta cgggacagga
     8221 tcatcgcggc gatgatataa ggcttccagc tggcttcttt gtaggtagcg atacggtatt
     8281 tttcgaatac gccttcagtc acttcccctt ctttgcagga tacgccgctg atatcagctg
     8341 gcaggcagtg catgtacagg gcttcgccat cacgggtcag ttccatcatc tcttcagtac
     8401 aatgccagtc tttgtgttgc gcgttctgtg ccagacactg tttttccagt gcttttaagc
     8461 cttcgtgatc gttagcgcgc agcaattcgg tacgctgttc catcactttg taaggtgccc
     8521 atgacttcgg ataaacgatg tctgcatctt tgaacgcttc ttccatgctg gtgacctgac
     8581 ggaagctacc gccagatgct ttagcattgt ttttcgcgac ttctactaca tccgggatca
     8641 ggtcgtagcc ttccggatgg gccagggtga catccatacc gaagcgagtc atcagaccga
     8701 tgatgccttg tggtacagag agcggtttgc catagcttgg agagtaggcc caggtcatgg
     8761 cgattttttt acctttcagg ttttccagtg aaccaaagtg ttcacgtaac catgccaggt
     8821 cagccattga ctgagtcggg tggtcaatat cgcattgcag gttcactaaa gccggacgct
     8881 gtggcagtac accctgcttg taaccgtcat caagtgcagc gccaacttca cgcatatagg
     8941 cgttgcctgc gcccagatac atatcgtcgc gaataccaat agcgtcggcg cagaaggaga
     9001 tcatattggc ggtttcacgc acggtttcgc cgtgagcgat ttgtgatttg ccttcatcga
     9061 gatcttgctg tgcaaggccg agcaggttaa gcgcggaagc ataagagaag cgggtacgag
     9121 tggagttgtc gcggaatacg gaaataccta atccactatt aaagactttg gttgagatgt
     9181 tttcagcacg cagtgctttt aatgctgcgg caacgtccag tacttgtttc agttcatctg
     9241 gagtctgttc ccacgttaac aaaaaatctt tctcgtgaag gtgagaggtc agcgaattga
     9301 tatccttaat cagctcatta acagttttca tcataaagtc ccggtagtgg aatgacaggc
     9361 aaagtggttt atttcactgt gacttgccaa agtgattggt gttttcacag ctatacctgg
     9421 gactaacaat aagtgtgcca gaattatcca cggggctgca aaagactaat ttgacggata
     9481 atgtgtgggg ttgatcacaa attgatgcca gcttccggtt gtttgtaaaa tacgaaaacg
     9541 tttggctttt tttatcgcgt tatttatttg ctgctaagta tcagaaataa gataaaggga
     9601 caggctatcc ctcgtcagtt tgataaactc accgccataa ttctcatatt gatatagaaa
     9661 tgtgatatct cttccataat ttcattagaa agtgataaaa atcttatgtt tttatcaaag
     9721 ccaatttcgc ctgagttaat atgatcatga tctgtgaacc atcaacgtct tcaatgatcg
     9781 acttttaagt aagtctggaa ggtaaagcta tggagcttgc tactacgcag tcagtattga
     9841 tgcaaattca accgacaatt cagcgttttg ccagaatgct tgccagcgtt ttgcagcttg
     9901 aggttgagat cgttgatgaa aacttgtatc gcgtcgccgg aacgggcgcg tatgggaagt
     9961 ttcttggtcg ccagttgagc ggcaactcac gcctgctctg ccacgtcctg gaaacgaaaa
    10021 ctgaaaaagt tgtgacacag tctcgcttcg atcccctttg cgaaggttgc gatagtaaag
    10081 aaaattgccg cgaaaaagca tttctgggta cgcctgtcat tttacaggat cgttgtgttg
    10141 gggtgataag tttgattgcc gttacccacg agcaacaaga gcatatcagt gataatttac
    10201 gcgaattttc tgattatgtt cgccatatat ccaccatttt tgtttcgaaa cttctggagg
    10261 atcaggggcc aggagataac atcagtaaaa tattcgcgac catgatcgat aatatggatc
    10321 agggcgtatt agttgttgat gatgaaagtc gggttcagtt tgttaatcag actgccttaa
    10381 aaacacttgg tgttgtgcaa aataatatta ttgggaaacc tatccgtttc agaccattaa
    10441 catttgagag taattttacc catggacata tgcagcatat tgtttcgtgg gacgataaaa
    10501 gtgaattaat cattggtcaa ttgcataaca ttcagggccg acaattattt ttaatggcgt
    10561 ttcaccaatc gcataccagt ttttctgtag caaatgcacc tgatgaaccg catattgaac
    10621 aattggttgg cgagtgccgt gttatgcggc aattaaaacg actcattagc cgtattgcac
    10681 ccagcccatc cagcgttatg gtggttggtg aaagcggcac gggtaaagaa gttgtcgccc
    10741 gtgcaatcca taagttgagc ggaagacgga ataaaccctt tattgctatc aactgtgccg
    10801 cgattccgga gcagcttctg gagagcgaac tgttcggtta tgttaaaggc gcatttactg
    10861 gcgcttctgc caacggtaaa acagggttga ttcaggcggc gaatacgggc acgctgtttc
    10921 tcgatgaaat aggtgatatg ccattaatgt tgcaggctaa attactgcgc gctattgagg
    10981 cacgtgaaat tctgccgatt ggtgccagta gcccaataca agtcgacatt cgcatcattt
    11041 ctgcaactaa ccagaatttg gcccagttca ttgccgaagg taaattccgc gaagatctct
    11101 tctaccgact taatgttatc ccgataactc tgccaccgct gcgtgaacgt caggaagata
    11161 ttgaactact ggtgcattac tttttacatc tgcatacccg tcgtctggga tcggtttatc
    11221 ctggcattgc tcccgatgtc gtcgaaatat tgcgtaagca tcgttggccc ggaaacctgc
    11281 gcgagttaag caatttgatg gaatatctgg ttaacgttgt tccttcaggt gaagttatcg
    11341 acagcacgct attgccgcca aatctgctga ataatggcac aacggagcaa agtgatgtaa
    11401 cagaggttac tgaggcacac ctgtcactcg atgatgcggg cggcacggcg ctggaggaga
    11461 tggaaaagca aatgatccgc gaggcgcttt cacgtcataa cagcaagaag caagttgctg
    11521 atgaactggg catcggcatt gctacgctct atcgcaagat taagaaatat gagttgttaa
    11581 agacataaca tcagtgaatc cggcctgaat tcaggccgga tatcctttta cttcgttttc
    11641 tcgcaatcca gaactgtatt tacaatcatc tgataccccg tgcagcgaca aagatttccc
    11701 gccagtccgc gacgaatttc cgtaatggtt aatggcttct cgcgtggttt cgccagcatt
    11761 gccgtggtag ccataatcag gccaggcgta caaaacccgc actgcactgc gccggatttc
    11821 gcataagcct gctgaacatg agaaagtttt ccgcctttcg cttcaccttc cagcgtgcgg
    11881 atctcttttc cttcagccca ggcggcaagg tataagcaac tgtctattgc tgtgccgtcg
    11941 accagcaccg tacaggcacc acattcaccc acgcagcacc cttgtttgac acttagcagc
    12001 ccttgttcgc ggagtaattc cgagagcggc gtgcctggta cggcatgaag ctgaaaaggc
    12061 atcccgttaa tggtgcattc gatggtaatt gtttcgctgt gattcattgc aattttcccc
    12121 ccgccgcggc gacggcttcg ctaatcactt ttttggtcat cgtctggatg agatgcagac
    12181 gaaactcttt actggcccgc catgaagaac gcggggcgac atcttgcagg acagattcgc
    12241 tgatagcttc cagcgtttgc aggtttaatg gcgcattttg tgcagtctgt tcggcatgtt
    12301 ggcagcgaat cggcgttggc gcggcaacac caaatgccag gcgtaattcg ctgaaattgc
    12361 cgttatccag tcggcaatgt gcggcacagc caatcgttga aatatccatt gcgtcgcgca
    12421 tggcatattt aaaatgcgcg ctgcccacgt gttctttcgg ctggggcgga aaatgaaagg
    12481 cgacaaggat ttcgtcatgc tcaagagaca ctttgcccgg cccggtgtga aagccattaa
    12541 tcgggacgaa acgaacaccg cgtggggagt ggatctccag tttcgcgtca taaatgagcg
    12601 ttggcgtggc agaatctgcg ctggtggcac cgttgcaaat atttccaccg taggtagcga
    12661 cgttacggat ctgcggtcca gcaatggacg aggccgcagc acataacgcc gggagatgac
    12721 gttgagttat agaatcttct attagctggg taaatgtcgt tgcagagccg atacgtagcg
    12781 agccatcttc cgccagcgta attccccgca gctccgccag attatgaata tcaacaatat
    12841 ggcgataacg gtcattgtga tggtggagct gaatcagtac gtcagtgcca ccggcgagca
    12901 gtttggcctg cgggttgtca gccagcaggt tgatggcatc ggcaagggtt gctgcgcgat
    12961 ggtaagaagc aaaatcaaac atgatgttat cctcaaatca atcctgccag atggaactct
    13021 tcatataacc gttttggcgt cagcggcagt gtattgattg caacaccggt agccatcttc
    13081 accgcgttac gaatagcagc ggcaacagga attattggtg gctcacccag tgacttatgt
    13141 ccgtatgcgg attgcggctc attgatttcg acgaacgcgc tttccagttg tggcagatcc
    13201 ggcatggtcg gcattttgta atccagcaga ttggggttac ggaccacgcc gcttttcgca
    13261 tcgatgatca tctcttcaaa tagcgcccag ccaatgccca ttcccattcc gccgtgtacc
    13321 tgaccttctg ccagtagcgg attaagaata tggcccgaat catgaacgtt gaggatgcgg
    13381 ttgatggtga ctttgcacag cgcaatatcg accgtcagat caacaaaggt acagccgaac
    13441 gccggtgggt tagtggtggt tttgatggag ctttcagcag agagctgccc gccgcgttca
    13501 gggtggtaga aagcgtccat cgccaaatct tttaacgaca ttaacggctc ttccggtcgt
    13561 tcaaccagca cgatatggcc ttttatcagg gtcagattca tcgctgactg atgtagcatg
    13621 actgcggcgt gagcgatgat tttctctttt aataacagtg ccgcactgcg cagcgcaggc
    13681 gcggcaacat agctctggcg tgaggcaaat gcgccgggat cgaacggcgt aacgtcggta
    13741 tcttgtgttg aaataacgcg aacgtcgctg accggaaccc ccacggtttc tgccaccatt
    13801 tgcgagaaga cggtgtcggc accctgaccg atttccgtcg cgccgctttg cacgttgatg
    13861 gttccatcct gattcatcag aaggcgcgcg cctgctattt ctacgccgac aggccaggtg
    13921 ttagaggtgt agctaaaaca ggcgacgcca acgccgcggc gtaaattgcc ttgctggttc
    13981 tggcactctg cacggcgttt ttcccattca aagattttcc ggcctttttc aagacactcc
    14041 ggcaaccctg cgctgtaaat acgtttgccc gtgagcggat tagcatctcc ttcgcgtgag
    14101 gcgttgcgta aacgaatttc aacaggatca atacctaacg ctgtcgcggc gtcatcaagc
    14161 atagactcaa cggcaaatac gacttgtggc gcgccataac cacgcatcgc accagccgag
    14221 gggaggttgg tatagcaggt ctttgaactg taagcgtagg cacaacgagg ataaaggtaa
    14281 gcgactttat ttccccctgc agaagcgatg gagtgcccgt gagatacata agcgccggtg
    14341 ttagacagaa catccagact ataacctttc aatgttccgt cgcggttcac gcccatttgc
    14401 ccgtcaatag taaaagcgtg gcgggtacgg gttgcgagga aacactcttc acggctaagg
    14461 gaaactttca ccggaatgcc gccaagcttg ctggtcagga atgccgccat tggctcttcc
    14521 agtacatcct gtttattacc aaaaccgcca ccgacaaatg gtttgatgac tcgtacgcat
    14581 gaccagggaa tatccagcgc ctgaccaacc acgcggcgaa caatgtgcgg gatctgggtg
    14641 ctggaaacga tggtaattcg cgagtcatcc tccatccacg ccagcgatgt tacgctttcc
    14701 atgtgacaat gttgaataac gggggtctga tagtgcccct gtacctggta gtcggcggca
    14761 tcgattgttt gttggacatt gcccgtcgac atcgtgcttt gtttcagtaa attgccgccg
    14821 ttatggattg gtgcagcgtc ctctgccagc gccgcttctg gcgtggtgat aacgggtaat
    14881 tcttcccact caatgctgac caattgcgcc gctttttctg ccgtgagttc atcgcgggcc
    14941 acgacgatgg caacggcgtc accatgatga cgaacatggc gagttagcag tgcgcgatcg
    15001 gcggtatcgc gcttgttttc gtcaagtgtc catgcatgcc cagctgtagc gaatgggata
    15061 tcaggcacat cttcccaggt aaaaatcgcc agtacgcctg gtaaacttct ggcttgttca
    15121 tcattaatac ttacggcata accatgtgcg atagggctac gtacatattt cgcataacac
    15181 atgcccgcca taacataatc gtcagtatat cgtgcccgcc cggtgacctt agcaatggca
    15241 tcaacgcgca tgcatgattc acccgtagcg gttgcttccc gcgcttccat agaaatcccc
    15301 tcgatttgaa catttggttc ttatggcaaa gatttagtct aagcaggcaa gatttacgcc
    15361 agaaacggcg ataagcgtta agggaggaag gaagtgtgat gatgttaaaa aaacacagat
    15421 atgatacgaa acaggaccct gaataggggt agtgatacgt atcaatatga gtggcgataa
    15481 aatcaatgtg ataaattagg attattacgc atcaggatgg gaaatagggt aaaaaagtca
    15541 ggaacttcga tacctctaca gtgaatattt tgctgatgat acttcccttt gccaaatgaa
    15601 attaacctct ttctctatta catcgttttt ccagaagcaa gagattgggt atcaagaggc
    15661 tggctgctat gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
    15721 ctgaataaaa aatctctggg tatcgtgatg ttgttatcgg ttggactgct tttggcgggc
    15781 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
    15841 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg
    15901 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
    15961 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
    16021 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
    16081 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
    16141 aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag
    16201 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
    16261 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
    16321 gtgaaggctg ggcaaaaaat cgccaccatg gggagcacgg atgcggcatc tgttcgcctg
    16381 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgcctcag
    16441 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
    16501 agaagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt tcaatggtag
    16561 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccaatttatc
    16621 ttcacctcat caatctgcca tttttgctgt atctgatttt ctatattatc ttaattcaat
    16681 cgcttcagac ttttcgcaat ctctgtaaca ctcccccatt tttttgtaaa taaaaagcgt
    16741 ttacgcaatg caatcctttt gtaacaaccc aggtaaaata ccttattttc aagttgttaa
    16801 ataagtggtt atctacgctt ttaacaaacg attgatggaa tattttatga aagggaaatc
    16861 tgcattaact cttctgctcg ctggtatatt ttcgtgcggc acatgccagg cgacaggggc
    16921 agaagtgacg agtgaatctg tatttaacat tcttaattcc acgggggcag caacagataa
//
LOCUS       DAZKFZ010000090        16805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000090 DAZKFZ010000000
VERSION     DAZKFZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(60..1022)
                     /gene="pfkA"
                     /locus_tag="ACHOQW_002656"
     CDS             complement(60..1022)
                     /gene="pfkA"
                     /locus_tag="ACHOQW_002656"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9573717.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(1203..2105)
                     /gene="fieF"
                     /locus_tag="ACHOQW_002657"
     CDS             complement(1203..2105)
                     /gene="fieF"
                     /locus_tag="ACHOQW_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP9573718.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(2254..2754)
                     /gene="cpxP"
                     /locus_tag="ACHOQW_002658"
     CDS             complement(2254..2754)
                     /gene="cpxP"
                     /locus_tag="ACHOQW_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP9573719.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            2904..3602
                     /gene="cpxR"
                     /locus_tag="ACHOQW_002659"
     CDS             2904..3602
                     /gene="cpxR"
                     /locus_tag="ACHOQW_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP9573720.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            3599..4972
                     /gene="cpxA"
                     /locus_tag="ACHOQW_002660"
     CDS             3599..4972
                     /gene="cpxA"
                     /locus_tag="ACHOQW_002660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP9573721.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(5078..5752)
                     /gene="yiiM"
                     /locus_tag="ACHOQW_002661"
     CDS             complement(5078..5752)
                     /gene="yiiM"
                     /locus_tag="ACHOQW_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP9573722.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(5901..6884)
                     /gene="kdgT"
                     /locus_tag="ACHOQW_002662"
     CDS             complement(5901..6884)
                     /gene="kdgT"
                     /locus_tag="ACHOQW_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP9573723.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(7144..7764)
                     /gene="sodA"
                     /locus_tag="ACHOQW_002663"
     CDS             complement(7144..7764)
                     /gene="sodA"
                     /locus_tag="ACHOQW_002663"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP9573724.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            8049..9083
                     /gene="rhaT"
                     /locus_tag="ACHOQW_002664"
                     /pseudo
     CDS             8049..9083
                     /gene="rhaT"
                     /locus_tag="ACHOQW_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(9080..9928)
                     /gene="rhaR"
                     /locus_tag="ACHOQW_002665"
     CDS             complement(9080..9928)
                     /gene="rhaR"
                     /locus_tag="ACHOQW_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP9573725.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(10002..10838)
                     /gene="rhaS"
                     /locus_tag="ACHOQW_002666"
     CDS             complement(10002..10838)
                     /gene="rhaS"
                     /locus_tag="ACHOQW_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP9573726.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            11126..12595
                     /gene="rhaB"
                     /locus_tag="ACHOQW_002667"
     CDS             11126..12595
                     /gene="rhaB"
                     /locus_tag="ACHOQW_002667"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9573727.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            12592..13851
                     /gene="rhaA"
                     /locus_tag="ACHOQW_002668"
     CDS             12592..13851
                     /gene="rhaA"
                     /locus_tag="ACHOQW_002668"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9573728.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            14026..14850
                     /gene="rhaD"
                     /locus_tag="ACHOQW_002669"
     CDS             14026..14850
                     /gene="rhaD"
                     /locus_tag="ACHOQW_002669"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9573729.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            14860..15174
                     /gene="rhaM"
                     /locus_tag="ACHOQW_002670"
     CDS             14860..15174
                     /gene="rhaM"
                     /locus_tag="ACHOQW_002670"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9573730.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(15215..16609)
                     /locus_tag="ACHOQW_002671"
     CDS             complement(15215..16609)
                     /locus_tag="ACHOQW_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP9573731.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         4116 a         4390 c         4250 g         4049 t
ORIGIN      
        1 ggcatttttg cttctgtcat cggtttcagg ctaaaggaat ctgccttttt ccgaaatcat
       61 tagtacagtt ttttcgcgca gtccagccag tcgcctttga acggacgctt catgttttcg
      121 atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct gtatacctac gcaacgaccg
      181 ccgtaacctg ccagcagcag atcgatagcg taagcgccca tacgggaagc cagaatacgg
      241 tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca
      301 cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta
      361 atcgccacga tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg
      421 tcttcacggc tgaattcaac ttccggaacc acaacgaatt cacagccacc ggcaatggcc
      481 gcagccagcg tcagatcgcc acaataacgg cccatcactt ccaccacgga aatacgctgg
      541 tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg
      601 aagaaaccga tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcaggccg
      661 atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg
      721 ataaccacca gcgcatcgat cccacgtttt ttcaggtttt cgatagccac ggcgcggatg
      781 ttctcgtcgc ggaattccgg gaaacgcgca gaaccgagga acgtaccgcc acggttgatc
      841 atgtcagaaa cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag
      901 ccgtcataaa tgcccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga
      961 attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc
     1021 atgactacct ctgaactttg gaatgcaaaa taaaatctgt tgccggaagt cttcttgcac
     1081 atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc
     1141 aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact
     1201 gattatgaaa gcatagaccg tttaccctcc ctaggtacga cggaacaggg gtcctgatgg
     1261 ataatcacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc
     1321 atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg
     1381 gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc
     1441 acgatatcaa taatttcttg ccgttcctca tcaggtaatg cgcgatccag taatgactgt
     1501 accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca
     1561 aacagagcat cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgcg
     1621 ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc
     1681 gtccggcgca ccacccaacg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca
     1741 attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatat cagatgttga
     1801 atgcctgtca aaaacaggaa tagtgccgaa ccggagataa acatactttg cgccagcgcc
     1861 gcgagggact cagctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa
     1921 tatcgcacca ccaataaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc
     1981 gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc
     2041 agtgaagcca tcgccgtcgc agcaatcgcc gcccgactga ccagccgtcc ataagattga
     2101 ttcataaata ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg
     2161 cattaagcag caggcaaatt gaggataaaa aaaaccccca catcatgtgg gggaagacag
     2221 ggatggtgtc tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact
     2281 caatagcttc aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg
     2341 ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg
     2401 gttgcggact tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc
     2461 ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg acaagacgat gcattgtctc
     2521 cagttcgcta acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat
     2581 ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg
     2641 ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct
     2701 taatgaactg actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg
     2761 ctcccaaaat ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca
     2821 tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt
     2881 tctgcctcgg aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc
     2941 tgacttccct attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg
     3001 atggggaaca ggcgcttgat cttctggacg acagcattga tttacttttg cttgacgtaa
     3061 tgatgccgaa gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc
     3121 ctgtcattat gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg
     3181 gcgcagatga ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg
     3241 cgatcctgcg ccgttcgcac tggagcgagc aacagcaaaa caacgacaac ggttcaccga
     3301 cactggaagt tgatgcctta gtactgaatc caggccgtca ggaagccagc ttcgacgggc
     3361 aaacgctgga gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg
     3421 gtcaggtggt ttcccgtgaa catttaagcc aggaagtgct gggcaaacgc ctgacgcctt
     3481 tcgaccgcgc tatcgatatg cacatttcca acctgcgtcg taaactgccg gatcgtaaag
     3541 atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta cctgatggtt tctgcttcat
     3601 gataggcagc ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt
     3661 gatgttggtt ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag
     3721 cgaacagcgt cagggtctga tgattgagca gcatgttgaa gcggagctgg cgaacgatcc
     3781 gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc
     3841 aggacagcgt ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga
     3901 aatgcagatc attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa
     3961 aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta
     4021 ccaactttat ctgattcgtc cggccagcag ttctcaatcc gatttcatta acttactgtt
     4081 tgaccgcccg ctattactgc tgattgtcac catgttggtc agtacgccgc tgctgttgtg
     4141 gttggcctgg agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc
     4201 ccagggaaac ttacgccagc acccggaact ggaagcgggg ccacaggaat tccttgccgc
     4261 aggtgccagt tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcagcg
     4321 tctgctttct gatatctctc acgagctgcg caccccgctg acgcgtctgc aactgggtac
     4381 ggcgttactg cgccgtcgta gtggtgaaag caaggaactg gagcgtattg aaaccgaagc
     4441 gcaacgtctg gacagcatga ttaacgacct gttggtgatg tcacgtaatc agcaaaaaaa
     4501 cgcgctggtt agcgagacca tcaaagccaa tcagttgtgg agtgaagtgc tggataacgc
     4561 ggcgttcgaa gccgagcaaa tgggcaagtc gttgacagtt aacttcccgc ctgggccgtg
     4621 gccgctgtac ggcaacccga acgccctgga gagtgcgctg gaaaacattg ttcgtaatgc
     4681 cctgcgttat tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac
     4741 cattacggtg gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg
     4801 tccgttctat cggaccgatg aagcgcgcga tcgtgaatct ggcggtacag gtttgggact
     4861 ggcgattgtt gaaaccgcca ttcagcagca tcgtggctgg gtgaaagcag aagacagccc
     4921 gctgggcggt ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca
     4981 tttgtaggca ggataaggcg tttacgccgc atccggcatt tgagcaggat gcctgatgcg
     5041 acgctgatag cgtcttatca ggcctacact ccgggggtta ttttccccac aaccggcggg
     5101 aaaaatcttc gatcttgccg ctcagtcggc gcttttgcat cgttcgcgtc cagcttttcg
     5161 ataacccggc agcggagagt aaacggtgat actgatcgtc atcaaacggc atatgccatg
     5221 cgatggcagc agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagcggcg
     5281 cgtccgcaga cactttccct ggcgctatca cgctatacaa ccatcccact ttacccgtgt
     5341 tttgcatcaa ctgcgctata tcgctgatat caaaatggta attgagttta tagcaaggcg
     5401 agcgcggctg gctgacctga attaatgcct ctccccagcg gaaaatatcg ccaatataaa
     5461 cattactttc cgtgagaccg tcggttgaga gattttcgcc aaacgcaggc gcaacaaaca
     5521 actccgcctg ttccggaaat tcccgcgccc agtagagata atgctcacga ggataatgac
     5581 acagcgctct gtctggtccg ccgtgaacct ttttctccgc ctgctcgtca ccttccagcc
     5641 ccagctccgt tagcatcaac tcaccatcaa cctggatttt ggcaattgcg ctgggtttgc
     5701 cttcgggata agcctgaatc ttgcctgtgt atacatcaac cggatatcgc atctgttccc
     5761 tctccacgag aaatttcccc attagaccat atttatttta tgtcaggcgt gctgttttcg
     5821 cggtaagtat ccaggcagga actcaatagc aaaccggacg aacgttagtt catccggctg
     5881 tggggattaa ggaagcaggt ttatttcacc gtacctaaaa tttcgatttt cgccgctctg
     5941 gctttgactt tacgtgacca gatagaggta agaattggca ccagaatcga agtcacaatg
     6001 actgccgtcg ctaccagcga agttgctgcc ggagccatcg gtttaaacgc aggcaccatt
     6061 tctgcaatca gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc ggcaattccg
     6121 gcagtgccat cgccaccgcc aatcaattta tcggcgataa tcaacggaat accggtcacg
     6181 ataattactg ccacacccaa caggatcccc agcaaacctg tctgggcaat tacagtcaaa
     6241 tcaatggtat tgcctagtgc gaaggcaaag aatggaatca gcgtctgcac cgctttgctg
     6301 aaaaattctc gtaattcagg gtcaaggttc ccaagggcaa agcccaccag gaacggtaat
     6361 actgcgccga cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc
     6421 atcgtcatga gcggcccgga ctccaacgac atcaacacaa atgccccagc ttcttctttt
     6481 gtgccgtact gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc catcgccgcc
     6541 accagcgcca gcgttgaaag tccggcaaag aatccaactt caacaccatg ttccggaata
     6601 atgcgcgagg caattgccgc aaccacccac gcgacggcaa ttttagttac caccagcgta
     6661 ccggatttac gcagtaccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa
     6721 aaccacaccg ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa
     6781 tatttccccg cccccggcga gaaggtgtgg cacagtgcgc caaggaatag cgggacgagc
     6841 atcatccccc ccgggatttt ctcaatcaag cgttttatct gcatttccat cacctgtcgg
     6901 catattttta agggctgatg cgtaattcct caatggagca tcagtgttga tggaaacgaa
     6961 gatagacatt catttaatca ataaaagtga tcaaaaccac atcaactgaa acgctgtttt
     7021 atttttatcg gatcattgtt tttattttga aattgatcac aaaaaaacca ccgccattgg
     7081 cgatggttca ttttttaagc tgatatgcgg cgtaaacgcc tcactgcagc agacggcaaa
     7141 tgattatttt ttcgccgcga aacgtgccgc tgcttcgtcc cagttcacca cgttccagaa
     7201 ctctttgatg tagtccgggc ggcggttctg gaatttcagg tagtaagcat gctcccacac
     7261 atccaggccc agaatcggga agccggatgc gccagaaata gcttcaccca tcagcgggga
     7321 gtcctggtta gcagtagaaa ccaccgccag tttatcgcct ttcagcacca gccatgccca
     7381 gccggaacca aagcgggaag ctgccgcttt ctcaaattct gctttgaagt tatctacgga
     7441 accgaaatca cgttcgatag ccgctttcag gtcaccctgc agggtggtgc cttttttcag
     7501 acctttccag aacaggctgt ggttagcgtg accgccagcg ttgttgcgca gtacggtttt
     7561 cttgtctgct ggcagctggt ccagtttggt gatcaactct tcaaccggca ggttggcaaa
     7621 ttctggcagg ctttccaacg ccgcgttggc gttgttgacg taggtctgat ggtgtttggt
     7681 gtggtggatt tccatggtct gcttatcgaa gtgcggttcc agggcatcgt aagcatacgg
     7741 cagggatggc agggtatagc tcatattcat ctccagtatt gtcgggcggc cgattgttaa
     7801 tgccgcgtaa gcagttggtt cattatagtt aattaaatga tattgaaaat gattatcaat
     7861 gccgtacttt tcgtaagggt atggttttgc aggaaaatgc ccgagatgtg aagcaaatca
     7921 cccacttaat gccgtgattg ccagtaaatc gacaacggcg gcaacaggcg aaaggttaat
     7981 cgacagcacg atttttacac tcatctcgtc ggagatgtga cgcgacgaaa aatgatgagg
     8041 ataagaagat gagtaacgcg attacgatgg ggatattttg gcatttgatc ggcgcggcca
     8101 gtgcagcctg tttttacgct ccgttcaaaa aagtaaaaaa atggtcatgg gaaaccatgt
     8161 ggtcagtcgg tgggattgtt tcgtggatta ttctgccgta ggccatcagc gccctgttac
     8221 taccgaattt ctgggcgtat tacagctcgt ttagtctctc tacgctgctg cctgtttttc
     8281 tgttcggcgc tatgtggggg atcggtaata tcaactacgg cctgaccatg cgttatctcg
     8341 gcatgtcgat gggaattggc atcgccattg gcattacgtt gattgtcggt acgctgatga
     8401 cgccaattat caacggcaat ttcgatgtgt tgattaacac cgaaggcgga cgcatgacgt
     8461 tgctcggcgt tctggtggcg ctgattggcg tagggattgt aactcgagcc gggcagttga
     8521 aagagcgcaa gatgggcatt aaagccgaag agttcaatct gaaaaaagga ctggtgctgg
     8581 cggtgatgtg cggcattttc tctgccggga tgtcctttgc gatgaacgcc gcaaaaccga
     8641 tgcatgaagc cgctgccgca ctaggcgtcg atccactgta tgtcgctctg ccaagctatg
     8701 ttgtcatcat gggcggcggc gcgatcatca acctcggttt ctgttttatt cgtctggcaa
     8761 aagtgaagga tttatcgcta aaagccgact tctcgctggc aaaaccgctg atcattcaca
     8821 atgtgttact ctcagcactg ggcgggttga tgtggtatct gcaattcttt ttctatgcct
     8881 ggggccacgc ccgcattccg gcgcagtatg actacatcag ttggatgctg catatgagtt
     8941 tctatgtatt gtgcggcggt atcgtcgggc tggtgctgaa agagtggaac aatgcaggac
     9001 gccgtccggt aacggtgttg agcctcggtt gtgtggtgat tattgttgcc gccaacatcg
     9061 ttggcatggg catggcgaat taatctttct gcgaattgag atgacgccac tggctgggcg
     9121 tcatcccggt ttcccgggta aacaccaccg aaaaatagtt actatcttca aagccacatt
     9181 cggtcgaaat atcactgatt aacaggcggc tatgctggag aagatattgc gcatgacaca
     9241 ctctgacctg ccgcagatat tgattgatgg tcattccagt ctgctggcga aattgctgac
     9301 gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt atccagcgca aagggacttt
     9361 tcaggctagc cgccaaccgg gtaatcagct tatccagcaa cgtttcgctg gatgttggcg
     9421 gcaacgaatc actggtgtaa cgatggcgat tcagcaacat caccaactgc ccgaacagca
     9481 actctgccat ttcgttagca aacggcacat gctgactact ttcatgctca agctgaccga
     9541 taacctgccg cgcctgtgcc atccccatgc tacctaagcg ccagtgtggt tgccctgcgc
     9601 tggcgctaaa tcccggaatc gccccctgcc agtcaagatt cagcttcagc cgctccgggc
     9661 aataaataat attctgcaaa accagatcgt taacggaagc gtaggagtgt ttatcgtcag
     9721 catgaatgta aaagagatcg ccacgggtaa tgcgataagg gcgatcgttg agtacatgca
     9781 gaccattacc gcgccagaca atcaccagct cacaaaaatc atgtgtatgt tcagcaaaga
     9841 catcttgcgg ataacggtca gccacagcga ctgcctgctg gtcgctggca aaaaaatcat
     9901 ctttgagaag ttttaactga tgcgccaccg tggctacctc ggccagagaa cgaaattgat
     9961 tattcgcaat atggcgtaca aatacgttga gaagattcgc gttattgcag aaagccatcc
    10021 cgtccctgac gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag cgtcgaaaag
    10081 tggttactgt cgctgaatcc gcaatgatag gcgatgtcag taacgctggc ctcgctgtga
    10141 cgtagcagat gtcgggcttt catcagtcgc aggcgattca ggtatcgctg aggcgtcagt
    10201 cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg aaagagaaaa ttgatccgcc
    10261 acggcatccc aattcacctc atcggcaaaa tggtcctcca gccaggccag aagcaagttg
    10321 agacgtgatg cgctgttttc caggttctcc tgcaaactgc ttttacgcag caagagcagt
    10381 aattgcataa acaagatctc gcgactggcg gtcgagggta aatcattttc cccttcctgc
    10441 tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata cgctgtggtt aacgcgccag
    10501 tgagacggat actgcccatc cagctcttgt ggcagcaact gattcagtcc ggcgagaaac
    10561 tgaaatcgat ccggcgagcg atacagcaca ttagtcagac acagattatc ggtatgttca
    10621 tacagatgcc gatcatggtc gcgcacaaaa cagaccgtgc caccggtgat ggtatagggc
    10681 tgcccattaa acacatgaat acccgtgcca tgttcgacaa tcacaatttc atgaaaatca
    10741 tgatgatgtt caggaaaatc cgcctgcggg agccggggtt ctatcgccac ggacgcgtta
    10801 ccagacggaa aaaaatccac actatgtaat acggtcatac tggcctcctg atgtcgtcaa
    10861 cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac ggaaaaccac
    10921 gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg gtgagcatca
    10981 catcaccaca attcagcaaa ttgtgaacat catcacgttc atctttccct ggttgccaat
    11041 ggcccatttt cctgtcagta acgagaaggt cgcgaattca ggcgcttttt agactggtcg
    11101 taatgaaatt cagcaggatc acattatgac ctttcgcaat tgtgtcgccg tcgatctcgg
    11161 cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt gaatgccgca gcctgacgct
    11221 gcgcgaaatc catcgtttta acaatgggct gcatagtcag aacggctatg tcacctggga
    11281 tgtggatagc cttgaaagtg ccattcgcct tggattaaac aaggtgtgcg aggaagggat
    11341 tcgtatcgat agcattggga ttgatacctg gggcgtggac tttgtgctgc tcgaccaaca
    11401 gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc cgcaccaatg gcctaatggc
    11461 gcaggcacaa caacaactcg gcaaacgcga tatttatcaa cgtagcggca tccagtttct
    11521 gcccttcaat acgctttatc agttgcgtgc gctgacggag caacaacctg aacttattcc
    11581 acacattgct cacgctctgc tgatgccgga ttacttcagt tatcgcctga ccggcaagat
    11641 gaactgggaa tataccaacg ccacgaccac gcaactggtc aatatcaata gcgacgactg
    11701 ggacgagtcg ctactggcgt ggagcggggc caacaaagcc tggtttggtc gcccgacgca
    11761 tccgggtaat gtcataggtc actggatttg cccgcagggt aatgagattc cagtggtcgc
    11821 cgttgccagc catgataccg ccagcgcggt tatcgcctcg ccgttaaacg gttcacgtgc
    11881 tgcttatctc tcttctggca cctggtcatt gatgggcttc gaaagccaga cgccatttac
    11941 caatgacacg gcactggcag ccaacatcac caatgaaggc ggggcggaag gtcgctatcg
    12001 ggtgctgaaa aatattatgg gcttatggct gcttcagcga gtgcttcagg agcggcaaat
    12061 caacgatctt ccggcgctta tctccgcgac acaggcactt ccggcttgcc gcttcattat
    12121 caatcccaat gacgatcgct ttattaatcc tgagacgatg tgcagcgaaa ttcaggctgc
    12181 gtgtcgggaa acggcgcaac cgatcccgga aagtgatgct gaactggcgc gctgcatttt
    12241 cgacagtctg gcgctgctgt atgccgatgt gttgcatgag ctggcgcagc tgcgcggtga
    12301 agatttctcg caactgcata ttgtcggcgg aggctgccag aacacgctgc tcaaccagct
    12361 atgcgccgat gcctgcggta ttcgggtgat cgccgggcct gttgaagcct cgacgctcgg
    12421 caatatcggc atccagttaa tgacgctgga tgaactcaac aatgtggatg atttccgtca
    12481 ggtcgtcagc accaccgcga atctgaccac ctttacccct aatcctgaca gtgaaattgc
    12541 ccactatgtg gcgcggattc actctacacg acagacaaag gagctttgcg catgaccact
    12601 caactggaac aggcctggga gctggcgaaa cagcgtttcg cggcagtagg gattgatgtc
    12661 gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa tgcactgctg gcagggtgat
    12721 gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg gagggattca ggctactggc
    12781 aattatccgg gcaaagcgcg taatgccagt gagctacgtg ccgatctgga acaggctatg
    12841 cggctgattc cggggccaaa acggcttaat ttacatgcca tctatctgga atcagacacg
    12901 ccagtctcgc gcgaccagat caaacctgag cacttcaaaa actgggttga atgggcgaaa
    12961 gccaatcagc tcggtctgga ttttaacccc tcctgtttct cgcatccgct aagcgccgat
    13021 ggctttacgc tttcccatgc cgacgacagc attcgccagt tctggattga tcactgcaag
    13081 gccagccgcc gcgtttcggc ctattttggt gagcaactcg gcacaccgtc ggtgatgaac
    13141 atctggatcc cggatggcat gaaagatatc accgttgacc gtctcgctcc gcgccagcgt
    13201 ctgctggcag ctctggatga ggtgatcagc gagaagctgg atccggcgca ccatatcgac
    13261 gccgttgaga gcaaattgtt tggcattggc gcagagagct acacggttgg ctccaatgag
    13321 ttttacatgg ggtatgccac cagccgccag actgcgctgt gcctggacgc cgggcatttc
    13381 cacccgactg aagtgatttc cgacaagatt tccgccgcca tgctgtatgt gccgcagttg
    13441 ctgctgcacg tcagccgtcc ggttcgctgg gacagcgatc acgtagtgct gctggatgat
    13501 gaaacccagg cgattgccag tgagattgtt cgtcacgatc tgtttgaccg ggtgcatatc
    13561 ggcctcgact tctttgatgc ctctatcaac cgtattgctg cgtggatcat tggtacacgc
    13621 aatatgaaaa aagccctgct gcgtgcgttg ctggaaccta ccgctgagct gcgcaagctg
    13681 gaagcggcgg gcgattacac tgcgcgtctg gcactgctgg aagagcagaa atcgttgccg
    13741 tggcaggcgg tctgggaaat gtattgccaa cgtcacgata cgccagcagg tagcgaatgg
    13801 ctggagagcg tgcgggcata tgagaaagaa actttgagtc gccgcgggta aacactgccg
    13861 gatgcggcac aagtgcctta tccggcctac aggccggcaa tagttgtagg cctgataaga
    13921 cgcgacagcg tcgcatcagg cattgattgc cggatgcggc acgagcgcct tatccggcct
    13981 acgggtcgtg catctgacaa caccgaattt acaggaacac agaacatgca aaacattacc
    14041 cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg atgcctggct gaaaggctgg
    14101 gacgagcgca acggcggcaa cctgacgctg cgcctggatg acgccgatat cgcgctttat
    14161 cacgacaact tccacccaca accgcgctat attccgctca gccaacccat gcctttactg
    14221 gcaaatacgc cgtttattgt caccggctcc ggcaaattct tccgtaacgt ccagctcgat
    14281 cctgcagcta acttaggcgt cgtaaaagtc gacagcgatg gcgcgggcta ccatattctc
    14341 tgggggttaa ccaacgaagc cgttccaact tctgaacttc cggctcactt tctctcccac
    14401 tgcgagcgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg ccacgccacc
    14461 aacctgatcg ccctcaccta tgtacttgaa aacgacaccg cggtcttcac tcgccaactg
    14521 tgggaaggca gcaccgagtg tctggtggta ttcccggatg gcgttggcat tctgccgtgg
    14581 atggtacccg gcacggacga aatcggccag gcaactgccc aagagatgca aaaacattcg
    14641 ctggtgttgt ggcccttcca cggtgtgttc ggttccggac cgacgctgga tgaaaccttc
    14701 ggtttaatcg acaccgcaga aaaatcagcg gaagtgttag tgaaggtgta ttcgatgggc
    14761 ggcatgaaac agactatcag ccgtgaagag ttgatagcgc tcggccagcg tttcggcgtt
    14821 acaccactcg ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc
    14881 atgcaggtaa accccgacgc ccacgaagag tatcagcgtc ggcataatcc catctggccg
    14941 gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa
    15001 gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagaacggtg gaatgcggta
    15061 gccagcactg aaatctgcca acgttggtgg aaatatatga ccgatgttat gcccgctaac
    15121 ccggataaca gcccggtgag tagcgagctg caagaagtgt tttacctgcc ttaatgagtt
    15181 gcggtgccgg aaaatatcca gcaccgcgac ttcatcagaa gaagtattta aagcgaacgc
    15241 gtgcgccgta acggtcatca gtatcatcac cgtgtttttt atcgccgtcg ctatccagaa
    15301 tggagtaata cgtcccgagg taaatattaa agttctccat atccatcacg ttagcgaact
    15361 gataagacgc atgaatggtg tgaatggtgt acgtcccttc atcatcaatc cagcagtcgt
    15421 tatcacaaac tacaccgctg aattcatcaa tcttattgtg cgcgtagata taaccgagct
    15481 cgaaacgttt ccacagcgcg ttaatcccgg cagtgaaatc tttctcatta ttagcatcta
    15541 aataggcggt atttagatta accacaatgc cattgtccgg atccgttttc aggccgttcc
    15601 aggtcatgct cataccatag ccggtacgat cggactgatc gacaaaatta cccttgctat
    15661 cggtataacc ataagcatta tttaccacgt tcgcttccat cgctgcggaa acggtgaatt
    15721 cttccgtcgg cgaccaggca attactgggc gcagataagc aacatttttc tgctgctcca
    15781 tatcgcgtcc atgataatta ccgtcattat ataaagatgt tccgtcttca agtaacgtgt
    15841 ttaattcaaa ataccagtta tcgagttgtt tgctgacgag gaaattaccg ccagcgttag
    15901 agcgcccgcg gccctctttc atcatataga tataaccgct gccatcgtca taaagatcgt
    15961 tcgcagtatt accggaatgt tcaacaaagg tatcctgatt caatgggaac atatcgtagg
    16021 cttcaaaacg gcctactttg accttccagt cgttttcttt gccaaagaag aataccgcat
    16081 catcgatatt tactgaaccg tgcatatcac ccagcggttg tgcggagaac ccggcgaaat
    16141 aaccgttgtc catctttcgc ataccgtcaa aacctaacag aatacgacca ttgagattcc
    16201 actgttcatt agtgggatcg ctattcacat cagcgttggt cattgccagc aggccatgat
    16261 tactttccgc atccatatta aattctacgt cgccgtagat ttttaaatcg ccataacctg
    16321 aaagcaaaag attaggtgag gtggcatttt tctttgcaga ttccgccgtt gcctgtccat
    16381 ttaccggcgt attgccctga gcggccttaa tttcgcggat ctcagcggct tgttgtgtct
    16441 gcagctgttt aacctggctc tctgcttttg ccgcccgcat ttctgccgct tccaggcgtt
    16501 cttccagcaa tgccatgcgt tgttccagca ttaaatgctg tgtttttgcc ataccggtac
    16561 tacatgccaa taataaaccg atggctaaag ataatgtcga ttttttcatt atttacgttc
    16621 cctgaaataa aggagaccct acgctttcca aaaggaatac gtatatagag aaatataagg
    16681 tgtgcacttt cgaattttta atccaacttc agcaggccga tggatagtat cccgccgcat
    16741 aatatgagta aatccgctga tttcctggaa cgtgaggaat ataacaatag tgccgttttt
    16801 aacta
//
LOCUS       DAZKFZ010000091        16609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000091 DAZKFZ010000000
VERSION     DAZKFZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1046
                     /locus_tag="ACHOQW_002672"
     CDS             <1..1046
                     /locus_tag="ACHOQW_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9573732.1"
                     /translation="ELLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQQETEQNDQ
                     KAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQEIF
                     DGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTST
                     SRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDT
                     MKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWGNGANFDNTILRR
                     SYERQGSPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVS
                     AIWQKLIPSQADS"
     gene            1119..1370
                     /locus_tag="ACHOQW_002673"
     CDS             1119..1370
                     /locus_tag="ACHOQW_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9573733.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            1405..1592
                     /locus_tag="ACHOQW_002674"
                     /pseudo
     CDS             1405..1592
                     /locus_tag="ACHOQW_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(2067..2153)
                     /locus_tag="ACHOQW_002675"
                     /pseudo
     CDS             complement(2067..2153)
                     /locus_tag="ACHOQW_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2359..2685)
                     /gene="ynfA"
                     /locus_tag="ACHOQW_002676"
     CDS             complement(2359..2685)
                     /gene="ynfA"
                     /locus_tag="ACHOQW_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP9573734.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            2820..3161
                     /gene="ynfB"
                     /locus_tag="ACHOQW_002677"
     CDS             2820..3161
                     /gene="ynfB"
                     /locus_tag="ACHOQW_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP9573735.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3196..3756
                     /gene="speG"
                     /locus_tag="ACHOQW_002678"
     CDS             3196..3756
                     /gene="speG"
                     /locus_tag="ACHOQW_002678"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP9573736.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3759..4469)
                     /gene="ynfC"
                     /locus_tag="ACHOQW_002679"
     CDS             complement(3759..4469)
                     /gene="ynfC"
                     /locus_tag="ACHOQW_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP9573737.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4577..4882
                     /gene="ynfD"
                     /locus_tag="ACHOQW_002680"
     CDS             4577..4882
                     /gene="ynfD"
                     /locus_tag="ACHOQW_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP9573738.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5081..7507
                     /gene="ynfE"
                     /locus_tag="ACHOQW_002681"
     CDS             5081..7507
                     /gene="ynfE"
                     /locus_tag="ACHOQW_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP9573739.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7568..9991
                     /gene="ynfF"
                     /locus_tag="ACHOQW_002682"
     CDS             7568..9991
                     /gene="ynfF"
                     /locus_tag="ACHOQW_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP9573740.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            10002..10619
                     /gene="ynfG"
                     /locus_tag="ACHOQW_002683"
     CDS             10002..10619
                     /gene="ynfG"
                     /locus_tag="ACHOQW_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP9573741.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10621..11475
                     /gene="ynfH"
                     /locus_tag="ACHOQW_002684"
     CDS             10621..11475
                     /gene="ynfH"
                     /locus_tag="ACHOQW_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP9573742.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11518..12132
                     /gene="dmsD"
                     /locus_tag="ACHOQW_002685"
     CDS             11518..12132
                     /gene="dmsD"
                     /locus_tag="ACHOQW_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP9573743.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12290..13582
                     /gene="clcB"
                     /locus_tag="ACHOQW_002686"
     CDS             12290..13582
                     /gene="clcB"
                     /locus_tag="ACHOQW_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP9573744.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13535..14230)
                     /gene="bioD"
                     /locus_tag="ACHOQW_002687"
     CDS             complement(13535..14230)
                     /gene="bioD"
                     /locus_tag="ACHOQW_002687"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9573745.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(14355..15575)
                     /gene="mlc"
                     /locus_tag="ACHOQW_002688"
                     /gene_synonym="dgsA"
     CDS             complement(14355..15575)
                     /gene="mlc"
                     /locus_tag="ACHOQW_002688"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP9573746.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(15710..16603)
                     /gene="ynfL"
                     /locus_tag="ACHOQW_002689"
     CDS             complement(15710..16603)
                     /gene="ynfL"
                     /locus_tag="ACHOQW_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9573747.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
BASE COUNT         4312 a         4179 c         4098 g         4020 t
ORIGIN      
        1 aggaactgct tgccgtatca cgtggtgaat ttgttgaagg gattagcgac ccgaatgatc
       61 cgaaatgggt taaggggatc cagacccgcg attctgtgaa ccagaaccag caagaaacgg
      121 aacagaacga ccagaaagcg gaacaaaaca gcccaaatac gcaacaaaac gagccagaaa
      181 cgaaacaacc tgaaccagta gtgcaacagg aaccggaaaa gatctgcacc gcctgcggtc
      241 agagcggtgg cggcaactgc cctgattgtg gtgcggtgat gggtgacgca acataccagg
      301 aaatattcga tggagagaat cagcctgaag ttcaggaaaa tgatccggag gaaatggaag
      361 gtactgcaca tcagcacaag gagaacactg gcggcaatca gcatcatgcc agcgatagtg
      421 aaactggcga ggcgtcagat cccttaatta aggcgaacgg tcatcataat ctcacatcca
      481 ccagcagagc ggggattcat ctgatgatcg atcttgaaac catgggaaaa aatcccgatg
      541 ccccgattat ctcaataggt gcaatatttt tcgatccgca aaccggagat atgggaccgg
      601 aatttagtaa gactatcgat ctggatactg ctggcggagt cattgatcgg gacaccatga
      661 aatggtggct taaacaatca cgcgaagcgc aatctgccat tatgaccgat gaaatcccgt
      721 tagatgatgc actgttacaa ttgcgggaat ttatcgacga aaactccggt gaattttttg
      781 ttcatgtctg gggaaatgga gccaacttcg acaacacgat tttgcgccgt tcatacgaac
      841 ggcaggggag cccctgcccg tggcgttact acaacgatcg cgatgtacgc acaatcgttg
      901 agctggggaa agccatagac ttcgatgcca gaacggctat tccattcgaa ggtgagcgcc
      961 ataatgcact tgatgacgcc cgttaccagg caaaatacgt ttcagctatc tggcaaaaac
     1021 tgatcccgag tcaggctgat tcttaatgtt caactgtcgc cggttgtgac tggtattctg
     1081 caaccggcgc tcgtctgatg taagagataa agaaatcgat gagcgaagta atcatgattg
     1141 tctctcccgg caaatgggta tccgaagagc agttaattgc gctgaaagga ataaaaaaag
     1201 gtacgttaaa aaaggcccgg gaaaaatcgt ttatggaagg aagggaatat aagcatgtcg
     1261 ctcatgacgg tatgccatgg gataacagtc catgctttta caacctggaa gaaattgatc
     1321 gctggattga gcgccaggca tcagcaagac caagacgtca tcttacttga ctaaaagcca
     1381 cactaactaa tgagagaagt tgaaatgaaa tatccgacag gcgtggaaaa ccatggaggg
     1441 aaattacgta tctggtttgt ttataaaggc gtaagagtca gggaaaacct ggggttcctg
     1501 acacagcaaa aaacaggcgc gttgcaggtg agctacgcgc ctctgtttgt tacgcaataa
     1561 aaactggtgt tttcgactat gcaaaacagt ttccctcctc acgcaatctg gaaaaatttg
     1621 gtgaggcccg acaagattta accataaaag aactggctga aaaatttctg gcactgaaag
     1681 aaactgaagt cgcaaaaaca tcactcaaca cataccgtgc cgtcatcaaa aatatcctga
     1741 gcataatcgg tgaaaaaaat cttgcctcat cgattaataa agaaaaatta ctggaggttc
     1801 gtaaagagct actgactgga taccagatcc ccaaaagtaa ctatattgtt acacaaccag
     1861 ggagatcggc tgtaactgta aataattaca tgacaaatct taacgccgtg ttccagtttg
     1921 gtgttgataa cggttacctg gcagataacc cgtttaaggg gatctcgcca ttaaaggaat
     1981 caagaaccat tccggatcct ctttcgcggg aggaattcat ccgtcttatc gatgcgtgca
     2041 gaaatcagca agccaaaaat ttatggtgta ataccgtcct cagtggtgat ttttaatgtg
     2101 acgaaattac gccccggaca ggtaacaaaa acttcagcct ttacgatctt catgttcgat
     2161 tccttgcatc gcttgtcgtg atgcatgaaa tctacgcaac tgagctacta ccatataagt
     2221 atgaagatcg aaaaaagccg gagtgatcac aaaaaaaggc gtatatttgc gctgtgaatg
     2281 gttgacaaaa gatgaaatag aatacctttt gtcagctgac acttcctcgt atcttattga
     2341 taaaatggat ttatgttcct acgtgcgccc ccagcccaca acaatgatca acatgccgca
     2401 aagcgcaatc aacggccccg tccagtcata aagagtcagt ttcacgccat ccacaacgcg
     2461 cagccacatc aacgccgtgc agacataaac gccaccataa gccgcgtaaa cacgcccact
     2521 cgccgcagga tgcaacgtta acaaccagac aaacagcgcc agtgaaatcc ccgccggaag
     2581 caacagccag atactggcgt ttcgttttaa ccacaaccag ggcagaaagc atccaataat
     2641 ttcacacagc gcagtagcaa aaaatagtaa cgttgtttta atcatctttg tcacttattg
     2701 acatcatgta tagttatagg gcgacataat atcatcaata taaacaccct cctggtacgt
     2761 tttgcgttcg cagtggatgg tgtagaatca tctttaatca ttcatacagg gaatgaatta
     2821 tgaaaatcac tctcagcaaa cgaatcgacc tgctcgcttt tctgctgcct tgcgcactgg
     2881 cattgagcac aactgttcat gccgaaacta acaaactggt gattgagtct ggcgacagtg
     2941 cacaaagccg ccagcacgcc gctatggaaa aagagcaatg gaatgacacg ggcaatctgc
     3001 gccagaaagt gaataaacgc actgaaaaag agtgggataa agccgacgcc gcttttgata
     3061 accgcgataa atgcgagcaa agcgccaaca tcaatgccta ctgggagcca aatactttgc
     3121 gctgcctgga ccgtcgaact ggccgcgtta ttacccccta acctgttatt gatttaagga
     3181 atgtaaggac acgttatgcc aagcgcccac agtgttaagc tacgcccgct ggagcgtgaa
     3241 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     3301 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcatat tcacgatcag
     3361 agcgaacgac gctttgtggt ggaatgtgac ggcgaaaaag ccggtctggt ggagttggtg
     3421 gaaattaacc atgtacatcg ccgcgcagaa tttcagataa ttatctcccc ggagtatcag
     3481 gggaaaggtc tggcaacccg tgccgccaaa ttagcaatgg actatggctt caccgttctc
     3541 aatctctata agctgtatct gatcgttgat aaagagaatg aaaaagcgat tcacatttac
     3601 cgcaagcttg gcttttcggt tgaaggtgaa ttgatgcacg agttctttat taatggtcaa
     3661 tatcgtaatg ccattcgcat gtgtatattc cagcatcagt atctggcaga gcacaaaaca
     3721 ccgggtcaga ctctcctgaa gccgaccgca caatagcatt aataataatc gatcgtattt
     3781 ttgatggtgt aaacccgttc gacggcgggt tttactcctt catcaacaat gatcagctga
     3841 cagcccaccg gattggcgtg actgtcatat tcacagctct gttttacatt accaactcgt
     3901 tgattattca gtaaagtaac cgctgtgtaa tctaattttt tgatcgggtc cgttgatggc
     3961 gtggcgctga cagataatgt tttgtcgtta cttttcgtgg ttttacccag tggataaccc
     4021 tgatcatcat agcgatattc catctgcatt tgtttgctgc tggctttaat cacgaagcca
     4081 ttatcatcgg tttcccaact caccccggca gaaggtaatt ctgctagctg gcattttccc
     4141 tgtaaacgta ctctcttctc cagcgtctcg gcatcacggt aataattggc gtccagtacc
     4201 agagcgacca cggtattatt ttccagatcc agtaattcga gtgaatcaaa acagccttct
     4261 tccgacaaag tccccgaaac acgtttcgtc acttcacctt gctcatccat taatgtctga
     4321 gtgaaatctt ttaccggacc acgcagcgga tcaaaatcga attcattaga gaaactggcc
     4381 atctcaggag taaatgaaag tgttacctct gtgcggtcac atcctgtgag taatatcgcg
     4441 agtaagcatg gcagtaattt gtatttcaca tcagtcacca gagagtagag atgattctca
     4501 atcatagtag caaatacagt actttacacg tgaaatgcta tgcttaaaga agttatcttc
     4561 gcgttaggag cttatgatga aactctcaac ctgttatgcc gcgcttctgc tcgccctcgc
     4621 ctcacccgcg gtactcgccg cgccgggatc ctgtgagcgc atacaaagcg atatatcaca
     4681 gcgcattatc aataatggcg tacccgaaag cagcttcacg ttaagtattg tacccaatga
     4741 ccaagttgat cagcctgatt cccaggtcgt cgggcattgc gctaatgata cgcataaaat
     4801 tctctacacc cgcaccacca gcggtaacgt ctctgctccc gcgcagtcta cccaggatgg
     4861 cgcgcctgcc gaaccgcagt aatacattat cgactacacg ccggatacga cacatccggc
     4921 gatttggaca tacaacataa tcccaccata ttactcatac ccttctattg atatggatta
     4981 ataattatta accccaaatg ggtagactcc ctctattgtt agcgcgctaa atattcaata
     5041 tataaacttt tatataacga taaagaacag ggagtgagtt atgtccaaaa atgaacgaat
     5101 ggtgggcatc agccgcagaa cactcgttaa atctaccgcg ataggttctc tggcgctggc
     5161 tgcaggcggt ttttctttgc cgttcaccct gcgcagtgca gcggcagcag tacaacaggc
     5221 cagcgaacaa gtggtctggg gtgcctgttc cgtcaactgt ggtagccgct gtgcacttcg
     5281 tctacatgtt aaagataatg aagtggcctg ggtggaaact gacaacaccg gcagcgatga
     5341 gtatggcaac catcaggtac gcgcctgttt gcgcggtcgc tccatccgcc ggcgtattaa
     5401 tcatcccgat cgcttgaatt acccaatgaa acgcgtgggt aaacgcggtg aaggcaaatt
     5461 cgaacggatt agctgggatg aagccctgga tactatcgcc agtagcctga agaaaaccgt
     5521 cgaacaatat ggcaatgagg ctgtatatat tcagtactct tcggggatcg ttggcggcaa
     5581 tataacccgc tcttcgccat cagcctcggc ggtcaaacgc ctgatgaact gctacggcgg
     5641 ttcactcaac cagtatggct cctacagcac tgcgcaaatt tcctgtgcca tgccctacac
     5701 ctacggcagt aatgatggca acagcaccac ggatattgaa aacagcaagc tggtcgtgat
     5761 gtttggtaac aacccggcag aaacccgcat gagcggcggt ggcatcactt atcttcttga
     5821 aaaatcccgc gaaaaatcga acgcaaaaat gattgttatc gatccgcgat ataccgatac
     5881 ggctgctggt cgtgaagatg aatggcttcc tattcgcccg ggcaccgatg ccgcgctggt
     5941 tgcgggtatt gcctgggtat tgattaacga aaatctcgtt gatcaacctt ttctcgataa
     6001 atactgcgtc ggctatgacg aaaaaacctt acccgcagat gcacccaaaa atggtcacta
     6061 taaagcctat attcttggtg aaggtgacga taaaacagcg aaaacgccgc agtgggcttc
     6121 gcaaattacc ggtatcccgg tggaccgtat catcaaactg gcgcgtgaaa ttggcacagc
     6181 aaaacccgct tatatctgcc agggctgggg gccgcaacgc caggcaaacg gcgagctgac
     6241 cgcacgcgct attgctatgt tgcctattct gacgggcaat gtcggcatca gcggcggaaa
     6301 tagtggcgcg cgtgaatcga cctataccat taccatagag cgcctgccgg tgctggataa
     6361 tccggtcaaa acgtcaattt cctgcttcag ctggacagat gctatcgatc atggcccaca
     6421 aatgacggca atccgcgacg gtgtccgggg caaagataaa ctggatgtgc ccattaagtt
     6481 catctggaac tacgcgggaa ataccctggt taatcagcat tctgacatca acaaaacgca
     6541 tgaaattctg caggacgaat cgaaatgcga aatgattgtg gtcatcgaaa actttatgac
     6601 ctcatcggca aaatatgccg acattctgct gccagacctg atgaccgttg agcaggaaga
     6661 tattattcct aacgactacg ccggtaacat gggatatctc attttcctcc agcctgtcac
     6721 cagcgaaaaa ttcgaacgca aaccgattta ctggatcctg agtgaagtcg cgaaacgtct
     6781 tggacctgac gtctatcaaa agttcactga aggtcgcacg caggaacaat ggttacaaca
     6841 tctgtacgcc aaaatgcttg ccaaagatcc ggcgttaccg tcttacgacg aactgaaaaa
     6901 aatgggtatc tataagcgta aagatcccaa tggtcatttt gtcgcctaca aagcatttcg
     6961 tgacgacccc gaggcaaatc cacttaaaac accttccggt aagattgaaa tttattccag
     7021 caagctggcg gaaattgccc gtacctggga actggaaaaa gatgaagtga taagcccact
     7081 gccagtttat gcctcaacct ttgaaggctg ggactcccct gaacgtagta cctttccact
     7141 gcaactgttt ggtttccatt acaaatcccg tactcactcg acctacggca acattgatgt
     7201 cctgaaggct gcctgccgtc aggaggtgtg gatcaaccct atagacgcac agaaacgtgg
     7261 gattgccaac ggcgatatgg tgcgggtgta taaccatcgt ggcgaagttc ggctaccagc
     7321 caaagtgaca ccacgcattc tccctggggt tagcgctatg ggccagggag cctggcacga
     7381 ggccaatatg tctggcgata aaatcgacca tggcggatgt gtgaatacgc taaccactct
     7441 gcgcccttca ccactggcga agggaaaccc gcagcacacg aatctggtcg agatcgaaaa
     7501 aatataaccc actacaacca taaattctgg catgacattt tgtttgaaaa gcaataagtg
     7561 agtaatgatg aaaatccata ccacagaggc gctcatgaag gctgaaatca gccgtagaag
     7621 tctgatgaaa acctccgcac ttggcagtct tgcgctggca agcagtgctt tcactctgcc
     7681 attttcccaa atggtccgag cggcagaggc accggtagaa gagaaagcgg tctggagttc
     7741 ctgcaccgtt aactgcggga gccgctgtct gttacgtttg catgtgaaag atgacaccgt
     7801 gtactgggtg gagtctgata cgacaggtga cgacgtctac ggtaatcatc aggttcgtgc
     7861 gtgtttacgc gggcgctcta tccgccgacg gatgaatcat cctgataggt tgaaatatcc
     7921 catgaagcgc gtcggcaagc gcggtgaagg taaatttgaa cggataagtt gggacgaagc
     7981 cctggatacc atcagtgata atcttcggcg gatcctgaaa gattacggca atgaggctgt
     8041 acatgtcctg tacggaacag gcgtagatgg cggaaacatc accaactcaa acgtcccgta
     8101 ccgtctgatg aactcttgcg gtggttttct cagtcgctat ggcagctaca gtaccgcaca
     8161 gatcagtgcc gcaatgagtt atatgttcgg tgccaatgat ggcaacagcc cggatgatat
     8221 cgccaatacg aaactggtcg ttatgttcgg aaataacccg gcagaaacgc ggatgagcgg
     8281 tggtggtgtc acttactacg tcgagcaagc ccgcgaacgt tcaaacgcac gcatgatcgt
     8341 catcgatcca cgttataacg acacagctgc cgggcgtgaa gatgaatggc tgcccattcg
     8401 ccctggcacc gatggcgcac tggcctgtgc gattgcctgg gtactgatta gtgaaaacat
     8461 ggtcgatcag ccatttctcg acaaatattg tgttggttac gatgaaaaaa cgctgcccgc
     8521 caacgcacca cgtaacgcgc attataaagc ctatattctg ggcgaagggc ctgacggcat
     8581 agctaaaacg ccggaatggg cagcaaaaat caccagcatc ccggcagaaa aaattatcca
     8641 gttggcacga gagatcggtt cagcaaaacc tgcttatatt tgtcagggtt gggggccaca
     8701 acgacattcc aacggcgagc aaacatcccg cgctattgcc atgctttccg ttctcaccgg
     8761 taacgtcggc ataaacggcg gcaactcagg tgtacgcgaa ggtagctggg atctgggggt
     8821 agaatggttc ccgatgctcg agaatcctgt taaaacgcag atttccgtct ttacatggac
     8881 agatgccatc gaccatggta cggaaatgac cgcgacccgt gatggtgttc gtggaaaaga
     8941 aaaactggat gtccccatca agtttttatg gtgctacgcc agtaacacat tgatcaatca
     9001 acatggcgac atcaatcaca ccaatgaggt gcttcaggat gacagcaagt gcgagatgat
     9061 tgttggcatt gaccacttca tgacggcctc ggctaagtat tgcgatatcc tgttgcccga
     9121 cctgatgcca acagagcaag aagaccttat ctctcatgaa tctgcaggga atatgggcta
     9181 tgtgatcctc gcccaacccg caacctcagc aaaatttgaa cgcaaaccca tctactggat
     9241 gctgagtgaa gtcgccaaac gcttaggacc ggacgtttat caaaccttta ctgaaggtcg
     9301 cagtcagcat gaatggatca aatatctcca tgcgaaaacg aaggaacgta accctgagat
     9361 gcccgactac gaggagatga aaacgaccgg gatctttaag aaaaaatgcc cggaagaaca
     9421 ctacgtcgct ttccgcgcat tccgtgaaga tccacaggca aacccgttga aaacaccttc
     9481 ggggaaaatc gaaatttatt ctgaacgact ggcgaagatt gcagatacct gggaattgaa
     9541 aaaagatgaa attattcatc cccttcctgc gtatacccca ggttttgatg gctgggacga
     9601 tcccctgcgg aaaacctatc cactgcagtt aacgggcttc cactacaaag cgcgtaccca
     9661 ctccagctac ggcaatattg atgtgttaca gcaggcctgc ccacaagagg tgtggatcaa
     9721 ccccattgat gctcaggctc gcggtatccg tcatggcgat accgtgcggg tatttaacaa
     9781 taatggagaa atgctgattg ccgcaaaagt gactccgcgt attctgcctg gcgttaccgc
     9841 catcgggcaa ggtgcgtggc ttaaagcgga tatgtttggt gaccgggtcg atcacggcgg
     9901 cagtatcaat attctgacct ctcaccgccc ttcaccgctg gcaaagggaa acccgtcgca
     9961 cagcaatctt gtccagatcg aaaaggttta aggagtagcc catgaccaca caatatggat
    10021 tttttatcga ttccagccgc tgtactggct gtaaaacctg cgaactggcg tgcaaagatt
    10081 ttaaagatct tggcccggaa gtcagtttcc gccgtattta tgaatatgct ggcggcgact
    10141 ggcaggaaga taatggcgtc tggcaccaga acgtgttcgc ttattatctc tccatctcct
    10201 gtaaccattg cgatgacccc gcatgtacaa aagtctgccc cagcggcgca atgcataagc
    10261 gtgaagatgg ctttgtggtg gttgatgaag atgtttgtat cggctgccgc tactgccaca
    10321 tggcttgtcc gtacggcgcg ccacagtaca atgctgaaaa agggcacatg acgaagtgcg
    10381 atggttgtta ttcgcgcgtc gccgagggga aacaacccat atgtgtcgaa tcctgcccgc
    10441 tgcgtgcact tgagttcggt ccaattgaag agctgcgcca gaagcacggc actcttgctg
    10501 ccgtcgcgcc actgccgcgc gcgcatttca caaaacccaa tatcgttatc aaacctaacg
    10561 ccaacagccg cccgaccggc gataccactg gttatctggc taatccggag gaagtgtaaa
    10621 atgggaaatg gatggcatga atggccactg gttatcttta ccgtattggg tcaatgcgta
    10681 gtaggcgcgc tgattgttag cggaattggc tggttcgccg ccaaaactga cgcagaccgt
    10741 cagcacattg ttcgtggcat gtttttcctc tggctgttga tgggcatcgg gtttattgcc
    10801 tctgtcatgc acctgggatc gccactgcgg gcgtttaact cgctgaatcg aatcggagct
    10861 tctggtctga gtaacgagat tgctgccggt tccatatttt ttgccgttgg cggactatgg
    10921 tggctggtgg cggtcatcgg taaaatgccg caagcgttgg ggaaactctg gttacttgtc
    10981 agcatggcgc tcggcgtcat tttcgtctgg atgatgacct gcgtgtatca aatcgacact
    11041 gtgccaacct ggcataacgg ttacactacg ctggctttct tcgtgacggt attgttgagt
    11101 ggtccaatac ttgctgccgc aattctgcgg gcagcccgcg ttacatttaa taccacacca
    11161 tttgccatca ttagcgttct ggcgctgatc gcttgcgccg gggtgattgt gctgcaaggt
    11221 ttgtctctgt cgtctattca ctcatccgtg caacaagcca gcgcactggt accagattac
    11281 gcctccttac aggtctggcg tgtggtattg ttatgcgcag gtctaggatg ctggctttgt
    11341 ccgctcatac gtcgccgtga acctcacgtt gccggactta ttctggggtt aatcctcatt
    11401 cttggcggtg agatgatcgg gcgtgtgctc ttttatggcc tgcacatgac ggtaggaatg
    11461 gctattgcag gttaacaaat tggtgcgggg caacccgcac atttcaggat gttaggaatg
    11521 acccattttt cacagcaaga taatttttct gtcgcggcgc gcgtgttggg cgcgctgttt
    11581 tattacgctc cagagagtgc tgaagccgca cctctggttg cggtactcac cagtgacggc
    11641 tgggaaactc agtggcctct accagaagcg tcattagcgc cgctggtgac ggcttttcag
    11701 acacagtgtg aagagactca cgcccaggcc tggcagcgtt tgtttgtcgg cccgtgggca
    11761 ctgccgtctc cgccgtgggg gtcggtctgg ctggatcgcg aatctgtgct gtttggcgat
    11821 tcaacattgg cacttcgtca gtggatgcgc gagaaaggca ttcagtttga aatgaagcaa
    11881 aacgaaccgg aagatcattt tggttcactg ctgttgatgg ctgcgtggtt ggcggagaat
    11941 ggtcgccaga cagaatgcga agaattgctg gcatggcacc tttttccctg gtcaacacgt
    12001 tttcttgacg tttttatcga aaaagcagaa caccctttct accgtgcact gggtgaactg
    12061 gctcgcttaa cgctggcgca atggcaatca caactgttaa ttcctgtcgc ggttaaacca
    12121 ctctttcgat aggatcaccg taatattgcc ggatggtgac gattacgcgc gattcggcaa
    12181 caatacgtta cagtgtcacg ttttttgtct cttaaagcac gcactgcttt tgcagctggc
    12241 ctcttttgcc gcaaaatagt cgcccgtgtt tcattgccca tttctgctca tgcatcatct
    12301 acacacctat ccggatctgc gcactatgtt ccgccgtctg cttatcgcaa cagtcgtcgg
    12361 tattctcgcg gcctttgccg ttgccgggtt tcgtcatgcg atgctgctac tggagtggtt
    12421 gttcctcaat aatgactccg gcagtctggt caatgcagcg acaaaccttt ccccctggcg
    12481 acggctgcta actccggcgc tcggcggact ggcggcgggt ttgttgttga tgggctggca
    12541 gaaatttacc cagcaacgcc ctcatgcgcc gaccgattac atggaagcgt tgcaaaccga
    12601 tggtcagttt gattacgcag caagcctggt taaatcgctt gcctctctgc tggtagtaac
    12661 cagcggcagt gcaattggtc gcgaaggtgc gatgattctt ttagctgccc ttgccgcctc
    12721 ctgttttgcc caacgtttta cgccacgcca ggagtggaaa ttatggatcg cctgtggggc
    12781 cgcggcggga atggctgcgg cctatcgtgc cccgcttgct ggcagtttat ttatagccga
    12841 agtgctgttt ggcactatga tgttggcctc tctcggcccg gtgattattt ccgccgtcgt
    12901 ggcattgctg gttagcaatc tgattaatca tagcgacgcg ttactctaca gcgtacaact
    12961 ctcagtgacg gttcaggctc gtgactatgc gctgattatc agtacaggtg tgctggcagg
    13021 tctgtgcgga ccactgttgt taacgttaat gaacgcctgt catcgtggat ttgtgagtct
    13081 caaacttgcg ccgccctggc aactggcact aggcgggttg atcgtgggtc tgctttccct
    13141 gttcacacct gcagtgtggg gcaacggcta tagcaccgta caatcctttt taaccgcccc
    13201 gccactgtta atgatcattg ccgggatctt cctttgtaaa ctgtgtgccg tgctggcgag
    13261 tagcggttcc ggcgcacccg gtggtgtctt tacaccgacg ctatttatcg gtcttgccat
    13321 tggcatgttg tatggtcgta gtctgggatt atggttccct gatggcgaag aaattacgct
    13381 tttactcgga ttgaccggga tggcgacact gttggcggca accacgcacg cgccgattat
    13441 gtcgacgttg atgatatgtg aaatgaccgg ggagtatcag ctactccccg gtttattgat
    13501 tgcctgcgta attgcgtcgg taatctcgcg gacgttacac cgtgactcta tctaccgcca
    13561 gcacactgcg cagcatagct aagcggatgt attgccccag ttcacgctgt tcggcgcgcg
    13621 gcagataagg cagttcacca atgagcggtg ccggaagttt tttaccgagc acatcaatga
    13681 tttccgcata atgcgccagt cctgggttga ttcggttagc cacccagcca atgagcggca
    13741 gcccgtcgtt ggcaatcgcc tgagctgtta gcagtgcatg gttaatgcaa ccttcctgaa
    13801 taccgacaac catcaacacc ggcagttgtt cctgcaccac ccattcagag agtggacgca
    13861 aatcattcat cagactacgc cagccgccag tcccttctac cacgacatga tcgacttttt
    13921 cggtcaggtt tgccaggccg tttgaaatga gggtgtaatt gattgggcaa ctgtgcgcca
    13981 cgctactttc ttcttcgctt aacgcgatag gattaactgc ttcataaggc agttcgatgg
    14041 ttgaaacact ctgcaacacc agggcatctt tattacgcag cccttcgggt gtctctttgc
    14101 tccccttcgc tacgggttta tatcccgcaa ccgtttttcc ctgggaggct aacgcttgta
    14161 gcaatgcgcg ggaaaccacc gttttcccta cagaagtgtc tgtaccggta ataaagaaac
    14221 gcttcagcat cactaactcc accgttatgc ttcacaaata taaaccagga aaataattaa
    14281 ccttgaaagt ctaagttatg ctttcctggc ccaaattgag atagcgcaaa ttttggtaga
    14341 acagttaaaa aatgttaacc ctgcaacaga cgaatcaaca aagaaccgtt atacatcgcg
    14401 tcttttacca gcgcagcccc tgccatcgtg ccctggttag aaaactgagt actctcaacg
    14461 ctaatgtgct gactatacgc aggaagggcc tgctgacgga tgctatctga gatgaccggg
    14521 aagaggatat ctgccgcttt acttaacggt gagccaatca gtattttttg tgggttaaat
    14581 aaattcacca tgatggcaag aatgcgccct acatgcgcgc ccaccccggt aatgatgtct
    14641 tttgccagca gatcgccgcg caatgccgcc tgacacaatg agtccacggt taacggttgc
    14701 ccatgtaaca tcgagctcat ggattgattg agacgcagct gtgccagctc aagaatactg
    14761 tccacactgg cgatggtttc gaggcagccg tggttcccgc aataacagcg tttaccatat
    14821 gggtctacct gtgtgtgacc tatttccacg aggctactgc tgcctgcgtg tagcagatga
    14881 ccatcggtaa tgacgcccgc ccccacgttg tgatcgataa ccacctgaat cacatcgcgc
    14941 gccccgcgtg aggcaccaaa caaggcctct gccatcgtcc atgcgctgat atcatgctga
    15001 atataaaccg gaacgccggt atgctgctcc agagcctcgc cgagcggcat ctcttttaca
    15061 tcctcgtaga acggcatgcg atgtacaatg ccattttccg tatcaataat tcccggcagg
    15121 gttatggcaa tcgaagttag acgctcaagt tttttctggt ggcggataaa aaactgatcg
    15181 atatgggaaa tgatacgatc cagcaatggc gagtcatctt ttaacgccag ttcctgcgac
    15241 tcttccacca ccagtttgct gctcagatcg cgcagagcaa ggaaaatctc cccgcgacta
    15301 atgcgcagag aaagatagtg ccaggcttca gtttcaacca ccagccccac cgccggacgg
    15361 ccacggttcc ccgcttcttt gatttccagc tcttgcacca ggtgtgcttc gagcatctca
    15421 cggacaattt tagtgatact ggcaggagcc agctgcgcca gacgggaaag atcgatacgc
    15481 gagactggac caagctgatc aatcaggcga taaaccgcgc ccgcgttggt ctgctttatt
    15541 tgatcaatgt gcccaggctg gttttcagca accaccgcat actccctata ttttcgcgct
    15601 ccgaaataat ctgtaggcta tggtgaagca cttcaatacg tgtcgtcaaa tttttactta
    15661 ggcatgtgat taacagcaca tttttcgggc tttttcgctg aaatttccct cacctgagag
    15721 cattcagcag atgaatacga aagttacgcg cagctggact ttgttcatga tgtttcggcc
    15781 agaccaacca catttcagaa accgcatcct cttcagcaat cggcacccag cgcatttcgt
    15841 tgagctgaac acgtttaaat gacgcaggca aaattgaaac acccagaccg gcggaaacca
    15901 gaccgatgat ggtcattgcc tcgcccacct cctgagtgat gacgggcgtc aaatggtaac
    15961 gtcgcatcag cccgagaata tcgtcataca gccctgtccc gacgtgcgga tcaaaaaaga
    16021 caaagggttc tttcgccagt tcagccagcg ttacattcgg gttatttgcc aggggatgat
    16081 cgtgcgggat catcgccata agcggttcat ggacgatgac tgcgtgttca agcgtctccg
    16141 gtaacgctgt gttacgcagc aatcccatat ccagcgttcc ttcaatgagc ggagcgattt
    16201 gctcgcgagt gttcatttcg cgggtttgta aatggacatc gggataatct cggcgaaaca
    16261 gcgataacgt atcggacacc gcccgaataa aaggagccga cgaagtaaaa ccaatgcgca
    16321 actcccccgc ttcaccctga tgcagtcttt cagcgcgagc ggcagcgtca tccaccatag
    16381 acaggatttg ccgactatct gcaagaaact gttttcctgc tgccgtcagc aatacactgc
    16441 gattggttcg tgccagcagt cgggcaccaa tttgttgctc cagcgcctga atctgctgac
    16501 ttagcggcgg ttgcgaaata ttcaggcggg cagcggcgcg cccgaaatgc agctcttccg
    16561 caacagcaac aaagtaacgc agatgacgaa gttcaatatt catatttaa
//
LOCUS       DAZKFZ010000092        16570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000092 DAZKFZ010000000
VERSION     DAZKFZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(37..189)
                     /locus_tag="ACHOQW_002690"
     CDS             complement(37..189)
                     /locus_tag="ACHOQW_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9573748.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(453..1187)
                     /gene="cysH"
                     /locus_tag="ACHOQW_002691"
     CDS             complement(453..1187)
                     /gene="cysH"
                     /locus_tag="ACHOQW_002691"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9573749.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1261..2973)
                     /gene="cysI"
                     /locus_tag="ACHOQW_002692"
     CDS             complement(1261..2973)
                     /gene="cysI"
                     /locus_tag="ACHOQW_002692"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP9573750.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2973..4772)
                     /gene="cysJ"
                     /locus_tag="ACHOQW_002693"
     CDS             complement(2973..4772)
                     /gene="cysJ"
                     /locus_tag="ACHOQW_002693"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP9573751.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5091..5453
                     /gene="queD"
                     /locus_tag="ACHOQW_002694"
     CDS             5091..5453
                     /gene="queD"
                     /locus_tag="ACHOQW_002694"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP9573752.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5531..6802
                     /gene="ygcN"
                     /locus_tag="ACHOQW_002695"
     CDS             5531..6802
                     /gene="ygcN"
                     /locus_tag="ACHOQW_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9573753.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            6793..7053
                     /gene="ygcO"
                     /locus_tag="ACHOQW_002696"
     CDS             6793..7053
                     /gene="ygcO"
                     /locus_tag="ACHOQW_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9573754.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7070..7645
                     /gene="ygcP"
                     /locus_tag="ACHOQW_002697"
     CDS             7070..7645
                     /gene="ygcP"
                     /locus_tag="ACHOQW_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP9573755.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7793..8653)
                     /gene="ygcQ"
                     /locus_tag="ACHOQW_002698"
                     /pseudo
     CDS             complement(7793..8653)
                     /gene="ygcQ"
                     /locus_tag="ACHOQW_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8650..9429)
                     /gene="ygcR"
                     /locus_tag="ACHOQW_002699"
     CDS             complement(8650..9429)
                     /gene="ygcR"
                     /locus_tag="ACHOQW_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP9573756.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9407..10743)
                     /gene="ygcS"
                     /locus_tag="ACHOQW_002700"
                     /pseudo
     CDS             complement(9407..10743)
                     /gene="ygcS"
                     /locus_tag="ACHOQW_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10837..12291)
                     /gene="ygcU"
                     /locus_tag="ACHOQW_002701"
     CDS             complement(10837..12291)
                     /gene="ygcU"
                     /locus_tag="ACHOQW_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP9573757.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12361..13146)
                     /gene="ygcW"
                     /locus_tag="ACHOQW_002702"
     CDS             complement(12361..13146)
                     /gene="ygcW"
                     /locus_tag="ACHOQW_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9573758.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13465..14742
                     /gene="yqcE"
                     /locus_tag="ACHOQW_002703"
     CDS             13465..14742
                     /gene="yqcE"
                     /locus_tag="ACHOQW_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP9573759.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14769..16247
                     /gene="ygcE"
                     /locus_tag="ACHOQW_002704"
     CDS             14769..16247
                     /gene="ygcE"
                     /locus_tag="ACHOQW_002704"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9573760.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3921 a         4375 c         4177 g         4097 t
ORIGIN      
        1 caattcaata cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgctctct gatactcaac tcacataacg agtcgcctac
      121 catgagcgtg aatcccagta ctgtacaaca cagtactatg attgccacaa gggcatattt
      181 tgttagcatg tcttgcctcc ctgcaaagaa gagactaaca tccgaattgc tacgttcgaa
      241 cgcgagcctc aggttgatag aaatatcgcc tggggctttt gtccatctgg aacctcgcga
      301 atgcttaacg ccagacagcc tcaagcaccc gacgccattc tatacctgat aattcttcca
      361 gcggcgttca cgtttaaaat caggaatgct gctcgcaaaa taaaaacgcc cccatcatta
      421 ctgacagagg cgtttaattt atccggcaat atttaccctt cgtgtaaccc acattccctt
      481 tttaagccaa agaaacgcgt ttcttcttcc gccatgccgg gttcccattt acgggttgta
      541 tgggtatcgc ccaccgataa atatccttcg tcccataatg ggtgatattt caggccattc
      601 ttttgcagat actgatatat cgttcggtta tcccagtcga taatcggcag cactttaaat
      661 acgccacgct gaattgccag caccggtaaa ttggcgcgac tgccggattg ttcacggcgc
      721 aggccagcaa accaggtttg cgcgttaagt tctttcagcg cccggttcat cggttcgact
      781 ttgttgatgt cattgtactt ttcaatgcct tcaacgccct gttcccacag ttttccgtag
      841 cgtgcttcct gccaggctgc gctttcggta gcacggtaca ctttcaggtt gagcttgagt
      901 ttgtccgtta actcgtcaat aaagcggtag gtttccggga acaagtaacc cgtatcggtg
      961 aggatcaccg gaatatccgg gcgaatttga ttcaccagat gcaggctcac cgccgcctga
     1021 atgccaaagc tggaagaaag tacatattca ccgggcagat tatccagcgc ccaggctacg
     1081 cggccttcag cgtccagttt ttccagttgg gcgttagttt ccgccagcgc cagaatgcga
     1141 tctaccttcg gcagttcgtt cagggcgttt agatcgagtt tggacataga ttcctcactg
     1201 tttgccttgc ctgatgcgac gctcacgcgt cttatcaggc ctacaagacc gggctgatgg
     1261 ttaatcccac aaatcgcgcg ccggatcgag caccgggcga atgatgcccg cacgcaccgt
     1321 aaagtcgccg aagccttcac ccgcttcgcg ctctttcgcc cagcgcccta tcagttcatc
     1381 aagcgacgcc aggatttccg gctcggtgat gttttcttta tacatccgtg ggatacgtgt
     1441 cccaatgcgg ttgccgccaa gatgcaggtt gtagcgaccc ggcgctttac ccaccaggcc
     1501 cacttccgcc agcatcgcgc gaccacaacc gttcgggcag cctgttacac gcatcacgat
     1561 atgctcatcg ctgacaccat gtttcgccat taaattatcg atgttgtcga taaaagacgg
     1621 caggaaacgc tccgcttccg ccatcgccag cgggcaagtc gggaacgaga cgcaggccat
     1681 tgagttttca cgctgcggcg tgacggcatt cattaacccg ctctctttgg cgatcttctc
     1741 gatcttcgct ttctcgcttt ccggcacacc ggcgatgatc agattctggt tcgccgtaat
     1801 gcggaaatca cctttgtgga tcttcgcgat ctccagcagg ccggttttca gcggacgccc
     1861 cggataatca aggatgcgac cattttcgat aaacagcgtc aggtgccagt tatcatcaat
     1921 gcccttaacc cagccaatac gatctccgcg tccggtgaac tcatacggac ggatcggttc
     1981 aaatttgatc cccgcgcgac gctccacttc cgctttaaac gtctcaaccc ccacgcgctc
     2041 cagcgtgtat ttggttttgg catttttacg atcggttcgg ttaccccagt cacgctgagt
     2101 tgtcacgacg gcttccgcca ctgccagcgt atgctccagc ggcagatagc caaactcact
     2161 cgccgtgcgg gcgtaggttt tcttgttgcc gtgttcgata gaaagtccac cgcccaccaa
     2221 caggttaaag cccaccagct tgccgttttc ggcgattgcc acgaagttca tgtcgttggc
     2281 gtgcagatcg atatcgttct gtggcgggat cactaccgtg gttttgaatt tacgcggcag
     2341 gtaggtctgg ccgaggatcg gttcttcatc ggtggtggcg actttttcct gatcgagcca
     2401 gatctccgca tacgcgcggg tacgaggcag cagatgttca gagattttct tcgcccactc
     2461 gtacgcttct gcgtgcagct gcgactcgta agggttcgag gtgcagagta cgttacggtt
     2521 catgtcgtta gcggtcgcca gcgcatcaag accgaccgag tgcagcatct ggtgcaccgg
     2581 tttgacgttc tttttcagaa tgccgtggaa ctggaatgtc tggcggttgg tcaggcgaat
     2641 gctgccatag atggtgtttt caccggcaaa tttatcgatc gcctgccact gtttggtggt
     2701 gataactcca cccggcagac ggcagcgcaa cagcatcgca tggcgcggct ccagcttctg
     2761 ttcagcacgt tcggcgcgga tgtcgcggtc atcctgctga tacatgccgt ggaagcggat
     2821 cagcaggaag ttatcgccct taaagccgcc ggtcaggccg tcgtttaaat cttccgcaat
     2881 ggtgccgcgc aggtagttgc tttcaagctt catgcgctcg gcgtctgtca gttttccttc
     2941 gaccactaaa ggccctggat gtttttcgct cattagtaga catctcgctg ataacggcgc
     3001 tctacgcgca gctcacttaa aaattcatcc gccgcttcgg tgtccatgcc accaaattcg
     3061 gcaatcactt ccagaagtgc ctgctcaacg tctttcgcca tgcgattagc gtcgccgcag
     3121 acataaatgt gggcaccgtc attgatccag cgccacagct ccgcgccctg ttcgcgcagt
     3181 ttgtcttgta cgtaaacttt ttctttttga tcgcgcgacc aggcaagatc gatacgcgtc
     3241 agcacgccct ctttgacgta acgctgccac tccacctggt agaggaaatc ttccgtaaag
     3301 tgcgggttgc caaagaacag ccagttttta cccggcgctt cgtcagcggc gcgctgctgc
     3361 ataaaggcgc ggaacggcgc gatgccggtg cctgggccaa tcataatcac cggggtttcc
     3421 gggttagcgg gcaggcggaa gttatcgtta tgttcgataa atacgcgaac ttcgccctct
     3481 tcttccacgc ggtccgcgag gaagctggag gcaccaccgg cacgggcgcg gccttccacg
     3541 tcgtaacgca ccacaccaac ggtgacgtgt acttcgttct cgacttcctc ctgcgaggag
     3601 gcgatggaat acagacgcgg cgtcagcggg cgcagcagat taatcagcgc ttcggcgtcc
     3661 agttgcgccg gagagaagcg caccatgtcg acaatcggcg tagtcgcggc gtaatgctgt
     3721 aacttcgctt tatcgcccac cagcggcaac agcgtttcgc tgcgggtaag cgtggcatag
     3781 ttctcaacaa tattggcggt gttgacggtc agttcgaagt gccactgtag cgcttcgttc
     3841 agaggcaacg ttttgccctc gacggtgaca ggttcatcgc ctttcagcca cagcagttcg
     3901 acaagttctt tcaccagtgc cggatcgttc tgataccaga cacccagcgc gtcacccggc
     3961 tggtaacgca ggcccgagtc acctaagtca atttcgatat ggcgaacgtc tttttcagag
     4021 ttacgcccgg taattttctg gttaaccgaa aggctcgcta ccagcggcgc gtctttgctg
     4081 tacgggctgg tgtggatttc atttaccacg ccagtagcga cggattgcga aggtgccgcg
     4141 acaggcgcac gcgatttaag cgcatcaacc acgcgggcgc gccactcgct ggcagcagcc
     4201 tggtattcaa cgtcggcgtc gacacggtcg agcaggcgtt caccgcccag ttccgccagc
     4261 ttgctgtcga aatctttccc ggactggcag aaaaattcat aagaactatc gccgaggcta
     4321 aacacggcaa aagcggtatt ttccagcttc ggcgcttttt tggagaacag gaacttatgc
     4381 agcgcgacgg cttcttccgg cggttcccct tctccttgcg ttgacgtcac cacgataagc
     4441 agtttttcgc tggcgatttg tttgaattta tagtcgcccg cgttcaccag cttaacgttc
     4501 agttttgccg tcaataaatc atcgcgtaat gcttcagcaa cccggcgcgc attgccggtt
     4561 tgcgaagcag agataatagt tatacccggc atttctgcgg ctggcgctgg cgtcgctgca
     4621 agcgcagcag gctgctgatt gagcacgccc cagaaatagc cagaaaccca ggcaagctgg
     4681 gtgggagtta aatcggtcgt ggccgcctga aggcgtgcca gttgctccgg gttcaacgga
     4741 agcaacgcgg aaggtgggac ctgtgtcgtc atgcgtcgtt atgttccagt aagcaaagct
     4801 gtttctgcgc cctgtcagcg cccataaaac agaagagaag gtaaggttaa cggggtgaac
     4861 ggtgtggatt aaagacggga tagcgataac taataaccaa atcgactaac ctgttttagc
     4921 aatagtcttt aacaacaaaa tagattaacc aacctaatga aaaacaaatg aatttagcca
     4981 atcattaaga taaatcagcg attttgcgca acaaagtcgt tttagataat gcgaaaaaag
     5041 agcctttccg gtactctacg gcggttttat cgtcctgtag agaaattatg atgtccacca
     5101 cgttatttaa agatttcacc ttcgaagccg ctcaccgctt accacacgtc ccggaagggc
     5161 ataaatgtgg tcgcctgcac gggcattcct ttatggtgcg actggaaatt accggggaag
     5221 tcgatccgca tacgggctgg attatcgatt tcgctgaact aaaagcggcg tttaagccaa
     5281 cttatgagcg cctcgatcac cattatctca atgatattcc aggtctggaa aacccaacta
     5341 gcgaagtttt agcaaaatgg atttgggatc aggttaaacc cgttgtgccg ctgttaagtg
     5401 cggtgatggt aaaagaaacc tgcaccgctg gttgtatcta tcgcggcgaa tgataagagt
     5461 gtggcggcgg tcaatttccc ttaagtaacg ctatgttagg gtgttgtgtt caggatatct
     5521 ggggcatgac atggaagacg actgcgacat tattattatt ggtgccggta ttgcaggcac
     5581 cgcttgcgcg ttacgctgcg cgcgagcggg tttatccgtt ttgttactgg aacgcgctga
     5641 aatccccggc agcaaaaatc tttccggcgg gcggttatat acccatgcac tcgcggaact
     5701 cctccctcaa tttcatctga ccgcgcctct tgaacgacgc atcactcacg aaagcctttc
     5761 cctgttaacg ccggattgcg caacgacgtt ttccagctta cagcccggcg gtgaatcctg
     5821 gagtgtatta cgtgcacggt tcgatccgtg gctggttgcc gaagccgaaa aagaaggtgt
     5881 cgaatgcatc cctggtgcga cggtggatgc actgtatgaa gaaaacggca gggtgtgtgg
     5941 tgtcatttgt ggtgacgata ttctccgcgc ccgttatgtg gtgctggcag aaggtgccaa
     6001 cagcgtcctg gctgaacgtc acgggttagt gactcgtcct gctggcgaag cgatggcgtt
     6061 ggggatcaaa gaagtgctgt cgctggaaac atccgctatt gaagaacgtt ttcatctgga
     6121 gaataacgaa ggcgcagcgt tgctgttcag cggcggaatc tgtgatgact tacccggcgg
     6181 cgcatttctt tatactaatc aacaaacgct ctcgttaggg attgtttgcc cactctcttc
     6241 ccttacgcaa agtcgtgttc cggcaagcga gctgcttgct cgctttaaaa cgcatccggc
     6301 agtgcgcccg cttatcaaaa acacggaatc actggagtat ggtgcgcatc tggtgccaga
     6361 aggtggcttg cacagtatgc cggtacaata cgccggtaac ggctggctgc tggtgggcga
     6421 tgcgttgcgc agttgcgtca ataccggaat ttctgtgcgc ggcatggata cggcgctaac
     6481 tggcgcgcag gcggcggcac aaacactgat aagcgcctgc cagcaccgcg agccgcaaaa
     6541 tctgtttccg ctttatcatc acaacgtaga gcgcagcctg ctgtgggatg ttctacagcg
     6601 ttatcagcat gttccggtgc ttttgcaacg cccgggatgg taccgtacgt ggcctgcgtt
     6661 aatgcaggat atttcccgcg atttatggga tcagggtgat aaacctgttc caccgctgcg
     6721 ccagttactc tggcgtcatt tacgtcgtca tggcttgtgg aatctggcgg gcgatgttat
     6781 caggagtgtt cgatgtctgt agcccgtaat ctctggcgcg ctgctgatgc gccgcacatt
     6841 gttccggctg actccgttga gcgtcagatg gtacaacggt tgattaacgc ctgtccggta
     6901 ggtctttttt cgctcacacc ggaaggtgac ttacgcgttg attatcacgg ctgcctggag
     6961 tgtggcacct gccgtttgct gtgcgacgaa tccacactac aacagtggcg ctatccgcct
     7021 tccggattcg gcatcaccta ccgatttgga taaataataa ggataattta tgcccctctt
     7081 acacctgctc cgccagaatc cggtgattgc tgccgttaaa gataatgcca gcctgcaact
     7141 ggccatcgat tctgaatgcc agtttatttc cgtcttgtac ggcaatatct gcaccattag
     7201 tcaaatcgtt aaaaagatta agaacgctgg gaaatatgct tttattcatg ttgatttgct
     7261 ggaaggcgcg tcaaataaag aagtggtgat tcagtttttg aaactggtga ccgaggcgga
     7321 tggaattatc agcaccaaag catcgatgct aaaagcggca agagcggaag gttttttctg
     7381 tattcatcgc ctgtttattg ttgattcgat ttcgtttcac aacattgata agcaagttgc
     7441 gcaatcgaat ccggattgta ttgagatcct gccaggctgc atgcccaaag tgctgggctg
     7501 ggtgacggaa aaaatccgcc aaccgctgat tgctggcggg ctggtatgcg atgaagaaga
     7561 tgcgcgtaat gcgattaacg cgggtgtcgt ggcgctttcc accacgaata ccggagtctg
     7621 gacgttagcg aaaaaattgc tttgacggga taatcgtaac caattgaatt tggtttgatt
     7681 tgtaggccgc acgccacatc cgacattcag cgcctgatgc gacgcttaac acgtcttatc
     7741 aggcctacag gtccccagtc cagaaccgta ggcaggatca tccggcagca cgttattggc
     7801 attctgcgtc aatgtttgtc accagtgcct ccagcatcac tttccagtca tcaaccacgc
     7861 caacatctgc ctgtgaaaat accgctgcgc tggcgtcatg attaatcgcc accacaaact
     7921 tgctattgcg tacgcccgcc attaacgctg ctgcgccaga agcgccaaca acaatacaca
     7981 cctcaggtgc cagcagatgt ccggaaatac caatcacttt ttcagcatca acgccaccgt
     8041 tcatcactcg cgcccgactg tagcccactt cggctcccag cttttatgcc agcatagcga
     8101 tttcctggtt atctgcctct ccgccctgcc ccaccaccaa cacccgtctg gcctcagcta
     8161 atgggtcgcg ggtaacattt ttcaggttct ctatgctaac cagccagtcc ggaagcgcac
     8221 ctgggactat gttcagttgc tgcataccag agggcaacgt ggcattttta tctgctcccg
     8281 cctggcgcgc cagggaaaga catagcgggc gtttttctgt ttgcagcgtc gccgttagcg
     8341 catttcccca gtgggatttg cgcacgctta cgattggaat ctctaacgaa gtcacctggc
     8401 agatgcttgc accatgtaaa cgccaggcca gccgagtcga cagttcatcg ccaaacgttc
     8461 ccggcggaaa gagaacaacg ttcgcaggcg ttcgctgcca ctgttccacc agcgcatcca
     8521 gcacctgttc caccactacg ggctgtggtt ctatttgcca gtgcgccagg gtacaaccgc
     8581 tgaaatcctg cgccgccagc cagcccgcta atgcggcatt ctcctgattt attgtcacaa
     8641 ttgcgatatt catggctgca tcctttggcg taaatagtcc tgccatagct tttgcgcttt
     8701 ttctgctacc gtttgcccat caatcagcgt cgctccgcgt cgttgctctg ctcttgccag
     8761 ttgcagacac tgcatcgctg gcaattcggc agcaacagtt ttacggataa tttccgcttt
     8821 ccccgcagcc attcgctggc gcattcccgg tactggcaag gccacttcac cgcactgacg
     8881 cacggcaatc accgcaggca agcgaatccg acaacagcgc agcccatgtt cagtacgctg
     8941 ttcgagggtg ataaacggcg cgtcgagagt gaaacgctcc acctgggtga agcaaggcca
     9001 gcccagcatt tccgccagca aaaatggcgt ttgcccattc tgcccttcgc tgctttggca
     9061 gccggtaatt atcagatcca gcggattctg acgctgccat tcggcaatat ggcgagcgac
     9121 aaattccgac gcaaagcgca gatctgccgc cgtctccagc aatacagctt cctcaaaccc
     9181 aagagccatg agatagcgca gccagtgcaa cgcccgttca tcccccatgc tcaacgcggt
     9241 taaagacatc ggcgtaccgt tttttctctg cgccaacagc agcgcggcgg cagcctgttc
     9301 atcagcaccg agtaaacttc gcagtagcga aatatccggt ccgctattac catgagccgc
     9361 cgcctgccac tctttttccg ccagcattcc ggcatccggt tcggctttaa acgctaacag
     9421 aatgttcatt cgcacctcct acatttcccg ccgccaccag cgggagtgct ttggtttctg
     9481 gtgcccatag ccaggttaca acaaaaccag ccaacaacac tgccgccagg agcaataagg
     9541 tgacttgcat tccccactgc gccagcaccc acggcagcag gccagtactt accgccgccc
     9601 ccaaccgact catggcggtg gcaaaaccga cgcccagcga gcgaatgtca gtaggaaaac
     9661 tttccgcagg caaaatgccc accagattac tgactgccga aatggtggtg ctgaagagaa
     9721 caaaagcagc agcgttaatg aactgccgga aggcaaacag gccatcacta ccagcgttgc
     9781 cgccagcagc aaaaaacttc ccagcaaaaa tttgcgatgt gccagcaggt gcgtcagaac
     9841 taatcccagc agcgcgccca caattaacaa cgcattaagc atcaggctgg cagtcagcgc
     9901 atcttccagt ccaatagtct gggcgatagt tggcagccag gtataaatca caaaccatgg
     9961 gattacgagg cagacaaaga agacgctgtt aaacgccgtg cgccgccagt agcgcgaaga
    10021 gaacaaggtt ttgatgtgtt tatgggtcgc cgttgccact tcatcgccca gtaaaacatg
    10081 gggaccaaaa tagcgatgca cgatagcgtg agcttctgca aaacgcccct ggcgcagtag
    10141 ccagcgtggc gattctggcg ttccccagcg taataacgta atcaacaacg cgggcagagc
    10201 tgccgatgcc agcagccagc gccaggcctc cgggttttcg gaaataaagt gatgtccggc
    10261 aatacttgcc aacacatagc ctacggtcca caccacgctg aatgcgccca gcaaaacacc
    10321 gcgatggcgg cgcggggaaa attcagccag caaggtgtga cctactgagt aatcgcctcc
    10381 cagaccaatg ccgatcaaaa tgcgcagtcc aataagatgc tctggcgtgg tggcaaaaaa
    10441 ttgcaagaac gaagcgagcg taatcagcaa aaagctgaag gtgaagattt tttgccgacc
    10501 aatatggtcg gagatccacc caagaaccag gctgccaaga aacaaaccaa ggtgcgccga
    10561 gccgccgatc attcccgcca taaacggcgt cagttgcatg gcgggcgtaa gttgaataat
    10621 ggcgtaacca atgacgccga gaacataacc gtcggtcagg tgtgcgccga aagtgagcgc
    10681 agcaattcga cagtgaaaac ggttaagcgg taaatcatcc attcgcaccg gtgaagtgtt
    10741 catctttacc ccttcattgt ccggtaataa gaacggcttc tcgcctgaga agccgaaaag
    10801 gcaacgcccc atcatcgcgg aacgctgcct gacacattat ttttcaatcg gatagatagt
    10861 acccgtattc ataatgccat taggatcgaa ctgctttttc agcccttcca gcaacgccca
    10921 cgcgctgccg tgttccagct tgctccagtg aacgcgatgt ttaccgatac cgtggtggtg
    10981 caccatcgaa ccaccgaggc gaatggtttc ttcgcaaatg attttgttga gcggattgtg
    11041 gtacttgtcg atttcctctt ccggcttaca gttgacaacg ttgtagtcgt agacgaagta
    11101 catgttggtg ccgttctgat agctatgaga ggagtgaccg cccagcatgg tgatgtcgtc
    11161 ggcgtgcggg aactcggtac gaatacggtt aataacgctt tcgtagattt cgtggatgca
    11221 gctccagcag ccggacactt cggtagtaaa gcccatgttg ccggttttga ggatctgcac
    11281 acgttcggca gccactttat ccggtcccca gttcaggttg ttaaaccagg tttcgatcag
    11341 cttgctgtcc acgcgttggc attgcgggta gcgggcaacg atttccgcaa tcccttcgcc
    11401 cgtcgccttc gcaatgcgag ggttaccttc agccataaag atcagcacgc attttccgtc
    11461 ggcaaaatgg gtgaagtgtt gggtgccatc ttcagcgtca tacaaacgag cgatcgacgg
    11521 acgataccct tccaccatga tttcacgcag gatgttgaag ccggttttca tgtcttccag
    11581 gatatagccg tagaagaggt tgttttccgg ggtgaattta aagattttca ctgttacttc
    11641 agtgatatag cacaatgcac cttcgttgcc gatgatgatg tgacgaatgt ccgggccagc
    11701 cgcgcggcgt ggcacgtttt taatgcgtgt gacggtgcca tctgccaaca ctgcctccag
    11761 accaacgacc atatcttcga ttgcgccgta gagtgtggag aactgcccga tactgcgggt
    11821 tgctaccagg ccgcccatct gcgccagcgg ctttgactgc ggagaatgcc ccgtggtgta
    11881 acctttttca cgcaacgcgt tttccagcac ctccagcgga acaccacatt gcgccgtcgc
    11941 ctgcatattc tcaatatcaa tattaatgat ttgattcatg gcggaaccgt cgagcaccac
    12001 cgagttttct acaacagttt ccagcccacc ttcggtggcg gaagcaccgg tacgcggcac
    12061 accgttaatt ttgtgcgcat tcataaaatt cagcacacgg gatacttgct ctgtggaacc
    12121 gagttttacg accgctgccg gaatcggcaa agtataaatg ccatgaatat ccggaaattt
    12181 acgaaaacgg tcaatactgt tcttctttaa tactgtttca tcggtaatta cgcgatctgc
    12241 accaacaatt tcctttagct ggtcgacaat cgctgcgcga gataaagaca tagtaattcc
    12301 ttcctgataa taaaaatgga ataatcatta ataagagaaa acagaaacca ccgttatggg
    12361 ttagcgcact aaataaccgc catcgaccac taataaatgc ccgttgacat aattcgatgc
    12421 ctgacttgcg agaaacacgg ctgcgcccat taaatcctga gtatcgcccc aacggtttgc
    12481 cggaatatga tcaagaacgc gctgattggt ttctggatta ctgcgtgtcg ccagcgtaat
    12541 gtctgttgca taatagccag gggcgatacc atttacctga atattatatt gacctagttc
    12601 atcacaataa gctttggtga atccggcaag agcatgttta gtagcagaat atgcaggtga
    12661 ccattgtcca cctaagtaag agaacaatga acagatatta atgattttac cgcttttctg
    12721 cgggatcata atttttgccg cttcgtagct taactcgaat gcggcggtca ggttcacatc
    12781 aatcatcggg tcccagtcgg cacgaccgaa gtccagcacc ttattcagct tacaaatacc
    12841 ggcattgtta accagaatat caactgtacc gaaacgctca cagcaggcag cgataatctt
    12901 ctgcggcgcg ccttctgcgg tgatatccac ctgcatgaag tccacctcaa caccctgatt
    12961 ttcaatcatt tcctttgttt cgccgttatc tttgacaaaa cttggaataa agacatttgc
    13021 gcctgctttg gccaacgcca tggcaaatgc ctggcctaaa ccgctattcc cgccggtaac
    13081 aattgcggtt ttacctttca gggagaaaaa atccattgag aacgcattga gagattcgat
    13141 tgacatagtt agctccaatt tccgtaaggc aaaaaaaaag aaaggtaact tcccccgcga
    13201 gagggaagtt acctttctca ttatctctgg taactggaga taatttttag cataaggaca
    13261 taactgctaa tgtgatcgta atcacagtgt gatattcgta ttatcaaata gttttaaaaa
    13321 gcagaagcgt gattactctc acacaacacc tctgctttcc atgttattta ttatttcaat
    13381 tactagagac ctgagaaaag taatccacct gaaagggcgg cttacttttc tctttttttt
    13441 gtctattaca aatgggaata cgatatgcaa cacaactcat atcgccgttg gataaccctc
    13501 gcgataatta gttttagcgg cggcgttagt ttcgacctgg cttatttacg ttatatttat
    13561 caaattccca tggcgaaatt tatgggattc agcaataccg agataggttt aataatgagt
    13621 acctttggta ttgcggctat tattctttat gcccccagcg gcgttattgc cgataaattt
    13681 tcacaccgca aaatgattac ttccgcgatg atcattaccg gattactggg tctgttaatg
    13741 gcaacgtatc caccgctgtg ggtaatgctc tgtattcagg tcgcctttgc gataacaacg
    13801 attttaatgc tgtggtcggt gtcgattaaa gccgcatcgt tgcttggcga tcatagcgag
    13861 caagggaaaa ttatgggctg gatggaaggg ctgcgcggcg tcggtgtaat gtcgctggca
    13921 gtgtttacca tgtgggtttt ttctcgcttt gcaccggatg acagcaccag cctgaaaacg
    13981 gtcattatca tctatagtgt ggtttacatc ttgttgggga ttctgtgctg gttttttgtt
    14041 agcgataaca acaacctgcg cagtgccaat aacgaagaaa aacagtcatt ccagcttagc
    14101 gacatccttg ccgttttgcg tatcagcacc acctggtatt gcagcatggt gatttttggc
    14161 gtcttcacca tctacgccat tctgagttac tccaccaact atctgaccga aatgtatggc
    14221 atgtcgctgg tggcggcgag ctacatgggg attgtgatca acaaaatctt ccgcgcgctg
    14281 tgcggcccac ttggcggcat tatcaccacc tacagtaaag tgaaatcccc tacccgcgtg
    14341 atccaaatcc tttccgtact cggcctgctg gcgttaactg ccctgctcgt cacgaactct
    14401 aacccgcaat cggtcgcgat ggggattggc ctgattttac tgctgggatt cacctgttac
    14461 gcctcacgcg ggctgtactg ggcctgccct ggcgaagcga gaacaccgtc ttacattatg
    14521 ggcaccacgg taggtatttg ctcggtgatt ggattcctgc cggatgtctt cgtttaccca
    14581 attatcggcc actggcaaga cacccagccc gcagcagaag cctaccgcaa tatgtggctg
    14641 atgggcatgg cggcgcttgc catggtgatt gtctttacct ttttgctgtt ccaaaaaatt
    14701 cgtactgctg atagcgcccc cgcaatggcc agcagcaagt aagccaggaa aattacagga
    14761 gaaaaatcat gtcgaagaaa tacatcatag ggattgatgg cggaagtcag agcacaaaag
    14821 tggtgatgta cgatctggaa ggtaacgtgg tttgcgaagg caaaggttta ttgcagccga
    14881 tgcacacgcc agatgccgat accgcagaac atcctgacga cgatttatgg gcatcattat
    14941 gttttgccgg tcacgatttg atgagtcagt ttgccgggaa taaagaagat attgtcggta
    15001 ttggtctggg atccatccgt tgctgccgtg cgttattgaa agccgatggc acgcctgctg
    15061 cgccgttgat tagctggcag gatgcacgcg taacgcgtcc ctacgaacac actaatcctg
    15121 acgtggccta tgtcacctct ttttcgggtt atctgacgca tcgcttaacc ggcgagttta
    15181 aagacaatat cgccaactat tttggtcagt ggccggtgga ttataagagc tgggcatgga
    15241 gcgaagatgc tgcggtaatg gagaagttta atatcccccg tcatatgctg tttgatgtgc
    15301 aaatgccagg cactgttctt ggacagatca caccacaagc cgcgctggcg acacatttcc
    15361 cggcagggct gccggttgtt tgtaccacca gtgataaacc ggtagaagct ctgggagccg
    15421 gattactgga tgatgaaacg gcggtgattt cactcggtac ttatatcgcg ctgatgatga
    15481 acggcaaagc cttgccgaaa gatccggtgg cgtactggcc gattatgtct tctattccgc
    15541 aaacattgct gtatgaaggt tacggtattc gcaaaggcat gtggacggtg agctggctgc
    15601 gcgacatgtt aggcgagtcg ttaattcagg atgccagggc gcaggatctt tcaccggaag
    15661 atttactcaa caaaaaagct tcttgtgtgc cacctggctg taatgggctg atgacggtgc
    15721 tggactggct gaccaatccg tgggaaccgt acaaacgcgg gattatgatc ggctttgatt
    15781 ccagcatgga ttacgcatgg atatatcgtt cgatactgga aagcgtggcg ctgacgctga
    15841 agaacaatta cgacaatatg tgtaatgaaa tgaatcactt tgcgaagcat gtgatcatta
    15901 ctggtggcgg ttcgaacagc gatctgttta tgcagatttt tgccgacgtg ttcaaccttc
    15961 cggcacgacg taacgccatt aacggttgtg caagtctggg ggcagcgatc aatacggcgg
    16021 taggtctggg gctatacccg gattacgcaa cggctgtcga caaaatggtt cgcgtgaaag
    16081 atatctttat accgattgag agcaatgcca aacgctacga cgcgatgaat aaaggtattt
    16141 tcaaagccct aaccaaacat actgatgtga tcctgaaaaa atcgtatgaa gtgatgcatg
    16201 gggaattggg gaatgcggat tcgatccaga gctggtcgaa tgcgtaagtg aggaagaccg
    16261 ggcgggaaac tgcccggcct gaacatacct gaatggttat ccccgctggc gcggggaaca
    16321 taagttcgac aacttaaaaa atgataacaa gtccggttta tccccgctga tgcagggaac
    16381 ataataaaaa ctttaactag ttcggtttat ccctgttttt acaaggaata ttaactacac
    16441 tcgatgtctt tagaataaaa gacaatacga atcgcacttt atcaattttc acttaaagta
    16501 tgaactggat atctttattc ataagaacta ttcatcaaca gcgtatagag gcggttatgt
    16561 aaaaccactc
//
LOCUS       DAZKFZ010000093        16501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000093 DAZKFZ010000000
VERSION     DAZKFZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.093"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(152..1435)
                     /gene="yeaH"
                     /locus_tag="ACHOQW_002705"
     CDS             complement(152..1435)
                     /gene="yeaH"
                     /locus_tag="ACHOQW_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP9573761.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1548..3482)
                     /gene="yeaG"
                     /locus_tag="ACHOQW_002706"
     CDS             complement(1548..3482)
                     /gene="yeaG"
                     /locus_tag="ACHOQW_002706"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP9573762.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3918..4664
                     /gene="mipA"
                     /locus_tag="ACHOQW_002707"
     CDS             3918..4664
                     /gene="mipA"
                     /locus_tag="ACHOQW_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP9573763.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            4754..5608
                     /gene="yeaE"
                     /locus_tag="ACHOQW_002708"
     CDS             4754..5608
                     /gene="yeaE"
                     /locus_tag="ACHOQW_002708"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP9573764.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5659..6543)
                     /gene="yeaD"
                     /locus_tag="ACHOQW_002709"
     CDS             complement(5659..6543)
                     /gene="yeaD"
                     /locus_tag="ACHOQW_002709"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP9573765.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6627..7622)
                     /gene="gapA"
                     /locus_tag="ACHOQW_002710"
     CDS             complement(6627..7622)
                     /gene="gapA"
                     /locus_tag="ACHOQW_002710"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9573766.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7964..8377
                     /gene="msrB"
                     /locus_tag="ACHOQW_002711"
     CDS             7964..8377
                     /gene="msrB"
                     /locus_tag="ACHOQW_002711"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9573767.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8419..8691
                     /gene="yeaC"
                     /locus_tag="ACHOQW_002712"
     CDS             8419..8691
                     /gene="yeaC"
                     /locus_tag="ACHOQW_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP9573768.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9061..10137
                     /gene="ydjL"
                     /locus_tag="ACHOQW_002713"
     CDS             9061..10137
                     /gene="ydjL"
                     /locus_tag="ACHOQW_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9573769.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10164..11543
                     /gene="ydjK"
                     /locus_tag="ACHOQW_002714"
     CDS             10164..11543
                     /gene="ydjK"
                     /locus_tag="ACHOQW_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9573770.1"
                     /translation="MDQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGTPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11560..12603
                     /gene="ydjJ"
                     /locus_tag="ACHOQW_002715"
     CDS             11560..12603
                     /gene="ydjJ"
                     /locus_tag="ACHOQW_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFP9573771.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12624..13460
                     /gene="ydjI"
                     /locus_tag="ACHOQW_002716"
     CDS             12624..13460
                     /gene="ydjI"
                     /locus_tag="ACHOQW_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9573772.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13465..14397
                     /gene="ydjH"
                     /locus_tag="ACHOQW_002717"
     CDS             13465..14397
                     /gene="ydjH"
                     /locus_tag="ACHOQW_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9573773.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14407..15387
                     /gene="ydjG"
                     /locus_tag="ACHOQW_002718"
     CDS             14407..15387
                     /gene="ydjG"
                     /locus_tag="ACHOQW_002718"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFP9573774.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            15524..16282
                     /gene="ydjF"
                     /locus_tag="ACHOQW_002719"
     CDS             15524..16282
                     /gene="ydjF"
                     /locus_tag="ACHOQW_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9573775.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16400..>16501
                     /locus_tag="ACHOQW_002720"
     CDS             16400..>16501
                     /locus_tag="ACHOQW_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573776.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLT"
BASE COUNT         4284 a         3712 c         4163 g         4342 t
ORIGIN      
        1 ataatttttc cgaatgattg ttttctggaa ctaaattgtg taaaggttat gctgtcatat
       61 ttaaagcgat tgtaagctaa tgtatgtaat aaatgagata acttacagtg agctaaaaat
      121 cagccaggcg ataatgaccc ggctgatagt tttagtcttt agctgttgca ttttgcttat
      181 gaaacagttc acggaacacc ggataaatat catcctggtc gcggatatgc tgcatcgcaa
      241 agttgtcgaa agtagattgc agatgctcat attctcgcca cagtgtctga tgcgcgcgac
      301 gggtaatttc gatatagctg taataacgaa caacgggtaa taattttttc gccagaattt
      361 catggcaaag cggagagtca tcggcccagt tatcgccgtc cgatgcttgt gcagcgtaaa
      421 tattccactg tgccgggtta taacgctctt tcactacctc atccatcagt ttcagggcgc
      481 tggaaacaat ggtgccgcca gtttcctgcg agtagaaaaa ctcatgttca tcgacttctt
      541 tcgcctgggt atgatggcgg atgtatacga cttccacgtt cttatacgtt ctgctgagga
      601 acagatacag cagaatataa aaacgcttag ccatatcttt agtggattga tccattgaac
      661 cggaaacgtc catcaggcaa aacatcactg cctggctgga gggatcgggc cgcttctcgt
      721 agttcttgta acgtaaatcg aaggtgtcaa taaaagggac gcgttcaatt ttggcacgta
      781 attctgcaat ttctttgcgc agacgttcct cttccagcag ctgcgcaggt tcactgttgc
      841 tgatgatggc caaattctct tccagtgcat gaagttcccg ccgcttgcct gccgtcatgg
      901 ctgtgcgtcg cgccagtgag ttctgcaagg aacgcacaac gctgatattg gccggaacgc
      961 cgttagcggt ataacccgcc cgatgcgttt tatattcggt cagctggcgt tgttggtttt
     1021 gtttcagatt cggtaaggcc aaatcttcaa agagcagatc aagatactca tctttcgaaa
     1081 tctgaaagac aaattcatcc tgaccttcac catcctggct ggcctggccc tgaccactgc
     1141 cggaacctcc gccgccaccc tggggacgtt caattcggtc gttctgaacg aaatggtcat
     1201 tgcccggatg cacgcggtgg cgcagaccgc cacgcccctg atgaaacatt ggttcgctaa
     1261 tatcttccgt gggaatggat acggactcgc cgctgtcgac gtcagtcacc gaacgcttat
     1321 taatggcctc ggagatcgac tgtttaattt gcgctttata acggcgtaaa aaacgctggc
     1381 gattcaccat gcttttgttt ttgccgttca gacgccggtc aataaaccag gtcatatgcc
     1441 ccccgtacta catttgccaa ctgtatgcta cgcctgtgtt gccatttctc tggcccacgg
     1501 ttcgctgtcg ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta agacgattta
     1561 cgtacgcgca aataccattc gcacagtaaa cgcacctgtt tacgggtgta gcctttttcc
     1621 atcatacggt cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt tttggcgtta
     1681 aacgagataa ccggcaacag ctcctcggta ttggagaaca ttttcttctc gataaccgtg
     1741 cgcagttttt catagctggt ccagttcgga ttgcgtccgc tgttattggc tctggcgcgc
     1801 agtacgaagt taacaatctc gttgcggaaa tcttttggat tactgatccc cgccggtttc
     1861 tcgattttct ccagctcggc gttaagagac tcgcggtcaa acagctgccc ggtatccgga
     1921 tcgcgatact cctgatcctg aatccagaaa tccgcgtagg taacataacg gtcgaaaatg
     1981 ttttgcccat attcggaata ggattcaagg taggccgtct ggatctcttt gccaataaac
     2041 tcggcatatt tcgggatcag ataacctttc aggaactcca gatagcgttc tgcctgctct
     2101 tgtgggaact gctcgcgctc gatctgctgt tccaggacgt agaacagatg gaccgggttt
     2161 gccgccactt ctacatgatc gaagttgaac acgcgggaga ggatcttaaa cgcaaaacgc
     2221 gtcgacagac cgttcatacc ttcatcgaca ccggcgtagt cacgatattc ctgatacgac
     2281 ttggctttgg gatcggtgtc tttcagactt tcgccatcat aaacccgcat ctttgaataa
     2341 atgctggagt tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga cagtgtttcg
     2401 agagtgccag gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa tttctcgtag
     2461 attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta aacacgatcg
     2521 aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggactc gttcgagtgg
     2581 gcaagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt gtagttacct
     2641 tcctgagtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa catctcgaca
     2701 aattccatga tcccttggtt ggcgcggcac agcgcaccgg aatagccgta ggcgtccggg
     2761 tcattctgcg cgtaatgttc gagtttacga atatcgactt tcccaaccag cgcggagatg
     2821 tcctggttgt tctcatcacc gggttccgtt ttggcgatag caatttgttg cagaattgac
     2881 ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg caggcgtttt
     2941 gccgcccacg gcgacatgat ggtgccgaga taacggcgag gaatgccata ctctttttcc
     3001 agaatctgcg catcttcctg cggattgaaa agacagaacg gatgatcgtt gaccgggcta
     3061 cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga tttcagtcgc
     3121 tcagcaagcg atgatttacc cccacccaca ggccccagca gatacaggat ttgtttcttc
     3181 tcttccagcc cctgagccgc gtgtttcaga taagagacaa tctgttcaat cgcgtcttcc
     3241 atgccgtaaa actcttcaaa cgccggataa cgtgcaatga cccggttaga aaagagtcga
     3301 gaaagtctgg gttcctgggc tgtatcgacc atgacaggct caccgatagc catcaatagc
     3361 cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag aaactcctgc
     3421 agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc gaatatattc
     3481 atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat gaatagtaga
     3541 ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa ctctcatgcc
     3601 attttatcga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta agcgtagatg
     3661 gcaaatggaa aacttgccta cacggctttc ttaatttcaa tggcatatca acaactcatc
     3721 cataaatttt gcataattaa tgtaaagacc aggctcgcca gtaacgctaa attcatttgg
     3781 ctgtaagcgc ggtgtcatcc gcgtcaggaa aattaaacag ttactttaaa aaatgaaaac
     3841 gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct aaacatttgt
     3901 ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg cttatcgcaa
     3961 cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc gtaggtgtcg
     4021 ttgaacaccc atataaagat tacgataccg atgtttaccc agtaccggta atcaactatg
     4081 aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg aatgacgcaa
     4141 cggataaact ttcaattacc gcttactggt cgccgcttta cttcaaagct aaagacagtg
     4201 gcgatcacca aatgcgtcac ctggatgacc gtaagagcac catgatggct ggtctgtctt
     4261 atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat accctggata
     4321 acagcaacgg catcgtctgg gatatggcct ggttgtatcg ttacaccaac ggtggcctga
     4381 ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa tactattatg
     4441 gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg ctataactcg aacgacagct
     4501 ggagccctta cctggagctg agcgccagct acaacttcct cggcgactgg agtgtttacg
     4561 gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccgatt gtggataaat
     4621 cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc atctttccag
     4681 ggcattttgt gcataaaaac agggcgtcac gccctgtttt gcattacggt ggtgcaatac
     4741 ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct cactgccagc
     4801 catagggcag ggaacatggt atatgggcga agatgccagt cagcgcaaaa cagaagttgc
     4861 tgcactacgc gcgggcattg aactcggttt aaccctcatt gataccgccg aaatgtatgc
     4921 cgatggcggt gccgaaaagg tggttgggga agcattaacc ggtctgcgag agaaggtctt
     4981 tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa atgcatgcga
     5041 agccagttta cgccgtctca atactgatta tctcgatctc tacttattac actggtctgg
     5101 cagtttcgct tttgaagaga ctgttgcagt gatggaaaaa ttgatcgccc agggaaaaat
     5161 ccgccgctgg ggcgtttcga accttgatta tgctgatatg caggaactct ggcagctgcc
     5221 ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac gagggattga
     5281 gtacgatcta ctcccctggt gccagcaaca gcagatgccg gtgatggctt acagtccgtt
     5341 agcccaggcc gggcggttgc gcaatggact gttaaaaaac gcggtagtca acgaaattgc
     5401 acatgctcac aatatcagcg cggcgcaagt attgttggcg tgggtaatca gccatcaggg
     5461 tgtgatggcg attccaaaag cggccacgat tgcccatgtc caacagaatg cggctgtgct
     5521 tgaggtcgaa ctttcgtcag cggaattagc catgctggac aaagcgtatc cggcaccaaa
     5581 aggaaaaact gcgctggata tggtgtgatg ttgatacgtg ccggatgcat cgcacccggc
     5641 acacaacatt gacgcaaatt aacgtttggc aacgcgaatg gattgcgcca gatgtgcagg
     5701 tttttctttg gtcacttttt gcatttctga ggcgtaagct gtttctacac aaacaaaggt
     5761 cttgtagcca tcgtccggca tgtcgcccat gctaactgaa agcgccggtc ccgggttcca
     5821 gcctacaacg ttcagatgat gctggtggcc tacggcgata atacgattca gcgcttcatc
     5881 attaatcacg ctgcaatctt gcggattcag atacacgcga tcggtacggt cagggaaggt
     5941 ctgaataccg tcggtcagta catcttcttt cgcgtcattt actttatcaa tgaagcgatc
     6001 gcccagccca ctgacgctta ccttagcgat atcacccacg ttaaagtagg tatgcagggc
     6061 agaggtggtt tcaaattcgc cgtgtgcttc aagatcgatt tcacagtttt tatccacgcg
     6121 gaaatgcgct aacagagtaa agtcgtgcag ccagaatttt ttcgtctctt cgctttgtgt
     6181 caattcaaaa gtcagcgcta cgccattagc atcttcacga tgtgatttca gcgtccacgg
     6241 caggttgcgg gcaaaaccgt gcgcaggcag gccttgttgt gccgccggac caaaccacgg
     6301 ccagcaaacc ggtacgccac cgcgaatagc aacgccattt ttgaacggtg tgttgttgct
     6361 caaccacaga acttcttctt cacctgcagg tttccacgag agaaggtgtg cgccctgtaa
     6421 tgcaaaagag gcttttacct ggggatgatc gactacaatg aggtccagtt catccagttt
     6481 acgacgggag aggacagggg agatttgttc gatgaccgga agggcaaaaa ttttcttaat
     6541 catgacgcag tcctttaact tcattttatc aggtaaaaaa aagagcgacc gaagtcgctc
     6601 tttttagatc acagtgtcat ctcaacttat ttagagatgt gagcgatcag gtccagaact
     6661 ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa gttgtcgttc
     6721 agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt gaaatcggta
     6781 gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc agcagcagct
     6841 ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac ggtcaggtca
     6901 actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt gccattcagt
     6961 tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat gatgttctgg
     7021 gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt tttctgagta
     7081 gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt atcgttgata
     7141 actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac gatgtcctgg
     7201 ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc tttagacgga
     7261 ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt ttcgtcagtc
     7321 aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca tttcaggtta
     7381 gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag atgaccgtct
     7441 ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag catgtatgcc
     7501 atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga acgtttctga
     7561 gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac tttgatagtc
     7621 atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt acaaaaacct
     7681 tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg tgcctaagca
     7741 ttacgcgact gactcgcctc actcttcctt tgggcttgag accacatggg gtcggcgccc
     7801 cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta ggtgcagcca
     7861 tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt tttgttagaa
     7921 tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta ataaaccttc
     7981 ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc agaatcatgg
     8041 gacagagccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg tatatcactg
     8101 tttgatctgc gatgccccgc tgtttcattc ccaaaccaag tatgattccg gctgtggctg
     8161 gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag acttgtcaca
     8221 tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg ggcatgtctt
     8281 ccctgacggg ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt ctttacgctt
     8341 cactgatggt gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag caacttattc
     8401 cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac gcctgaggta
     8461 taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt tgcgttgacc
     8521 gaggaacaaa aagagaactg cctgcaactg gtgatgctgt ggcaagcccg caataatacc
     8581 gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag caaacagcag
     8641 ttaaaagaag attttggtat ctcagcaaag cctattgcca tgtttaagta agtggttcat
     8701 cccctttttg ccgcggctat gaaggggata gccaacaatg accccacgac tcatctcaat
     8761 tatctcgcca tgacctggct aagtcccagt ccgatctctt gccgtcgcgt tcacgcctga
     8821 atgacttctc ctgaacttaa gtctcagacc tatttggccg gtaatccctc tcaaatttgc
     8881 tccttttgcg ttccgcctta catcaacctg taacagaacc agccattttc tgcgtaattg
     8941 tgatctgagt tgtaaaacgc agattattat ctgtgtttta catgtttaat gttgacctca
     9001 aaaatcatgt gggtaagtat gatgaccatc taagcaaaac aaaacacaga ggatgacgca
     9061 atgaaagcac tggctcggtt tggcaaagcc tttggcggct acaagatgat cgatgtgcca
     9121 caacccattt gtggcccgga agatgtagtg attgaaatta aagccgcggc aatctgcggc
     9181 gcagacatga agcactacaa tgtcgatagc ggttctgatg agtttaactc tatccgcggc
     9241 catgagttcg caggttgtat tgcgcaggtt ggtgaaaaag tcaaagactg gaaagtgggg
     9301 caacgcgtcg tgtcggataa cagcggtcac gtctgcggcg tttgtccggc ctgtgaacaa
     9361 ggtgattttc tgtgttgtac agaaaaagta aaccttggtc tggataacaa tacctggggc
     9421 ggtggttttt ccaaatattg tctggttcct ggtgaaattc tcaaaattca tcgtcatgcg
     9481 ttgtgggaaa tccctgatgg tgttgattat gaggacgcag ccgtacttga ccctatctgt
     9541 aatgcctaca aatccatcgc gcagcaatcg aaattcctcc ctggtcagga tgtggtcgtc
     9601 atcggcactg gcccactcgg gctgttctcc gtacaaatgg cgcgaattat gggggcggta
     9661 aatatcgtcg tcgttggtct gcaagaagat gtggcggtcc gcttcccggt tgcaaaagaa
     9721 ctgggtgcga cggcagtagt aaatggttct accgaagatg tggtggcgcg ctgccagcaa
     9781 atttgtggca aagacaatct gggactggtg attgaatgct ccggtgccaa tatcgcactg
     9841 aaacaagcca tcgaaatgct ccgtccgaac ggggaagtgg tacgcgttgg aatgggcttc
     9901 aaacctcttg atttctcgat taatgacatt accgcctgga acaaaagcat cattgggcat
     9961 atggcctatg actccacctc atggcgtaac gctatcaggc tattagccag cggcgctatc
    10021 aaagtcaaac cgatgatcac gcatcgtatc ggcctgtcgc aatggcgcga agggtttgat
    10081 gcgatggtcg ataaaaccgc aatcaaagtg atcatgactt acgactttga tgaataacca
    10141 ctctcctgaa ggaacgttat cgcatggatc agataacaaa accgcattgt ggtgcacggc
    10201 tggatcgctt accggattgc cgctggcatt catcaatgtt tgccatcgtc gcgtttggct
    10261 tgcttgtctg ctggagtaat gccgttggtg gcttgatcct cgcgcagctg aaagcgttgg
    10321 gctggacaga taattccacc acagccacat tctcagcaat cacgaccgcc ggaatgtttc
    10381 tcggtgcgct ggtgggcggc atcattggcg acaaaaccgg tcgcagaaat gcgttcatcc
    10441 tctatgaggc cattcatatt gcctcgatgg ttgtcggtgc tttctcaccg aatatggatt
    10501 tccttattgc ctgccgtttt gtgatgggag ttgggctggg agctttactg gttacgctgt
    10561 ttgctggttt caccgaatat atgcccggta gaaatcgtgg aacgtggtca agtcgggttt
    10621 cctttattgg caactggtca tatccgctct gttcattgat agcgatggga ctcacgccgc
    10681 tgattagtgc agagtggaac tggcgggtac aactgcttat ccctgcaata ttgtcgctta
    10741 tcgctacggc gctggcctgg cgctactttc ctgaatcccc gcgctggctg gaatcgcgcg
    10801 gacggtatca ggaagccgag aaagtcatgc ggagtataga agaaggcgtc atacgccata
    10861 caggtaaacc tttgccgccc gtggttattg ctgatgacgg taaagcacca caagcggtgc
    10921 catattcagc cttactgaca ggagtattac tgaaacgcgt gatattaggc tcttgtgtgc
    10981 tgattgccat gaacgttgtg cagtacacac taattaactg gttgccaacg atattcatga
    11041 cccaggggat taatttaaaa gactcgattg ttttaaatac catgagtatg tttggcacgc
    11101 catttggcat ttttattgcc atgctggtga tggataaaat tccgcgtaaa acaatgggtg
    11161 tggggctatt aatcctgatt gcggtgctcg gatatatcta ttcactgcaa accagcatgt
    11221 tgctcataac gttgattggt ttcttcctga ttactttcgt ctatatgtac gtttgctatg
    11281 cctcggcagt gtatgtccct gagatctggc cgacagaggc caaactccgt ggctccggtc
    11341 tggcgaatgc ggtagggcga atcagtggta ttgccgcacc ttatgccgtt gcagtgctgc
    11401 tcagtagtta tggcgttacg ggagtcttta ttcttctggg ggcggtttca attattgtcg
    11461 caattgctat cgccaccatt ggaatcgaaa ccaaaggtgt ctccgttgaa agtttaagta
    11521 ttgatgcagt agccaataaa taatacgggt ggattcaaaa tgaaaaattc aaaagcaata
    11581 ttgcaggtgc cgggcacaat gaaaattatt tcagcagaaa taccagtgcc taaagaagat
    11641 gaagttttga ttaaagtaga atatgtcggt atttgtggtt cagatgtaca tggttttgaa
    11701 tcaggtccgt ttattccgcc taaagaccca aatcaagaaa ttggcctggg tcatgaatgc
    11761 gccgggacgg ttgtggctgt gggaagccgt gtgcgcaaat ttaaaccggg ggatcgggta
    11821 aatatcgaac ctggcgttcc ttgcggtcac tgtcgttact gtctggaagg caaatataac
    11881 atctgcccgg acgttgattt tatggcgaca caacccaact accgcggcgc attaacgcac
    11941 tatctgtgtc atccggagag ctttacttac aaactgcccg acaatatgga cacgatggaa
    12001 gggacgctgg tggagcctgc cgcagtcggg atgcatgccg cgatgctggc agatgttaaa
    12061 ccgggtaaga agataattat tctgggagca ggttgtattg gtttgatgac gttgcaagcg
    12121 tgcaaatgcc tgggagcaac ggaaattgcc gtcgttgatg tgctggaaaa acgtctggca
    12181 atggcggaac agcttggtgc gacagtggtt attaacggcg caaaagaaga cactattgca
    12241 cgctgtcagc aatttaccga agacatgggc gcagatattg ttttcgaaac agcgggttct
    12301 gcggtcaccg ttaaacaggc accttatctg gtaatgcgtg gcggtaaaat tatgattgtt
    12361 ggtactgtac ccggcgcttc ggcaatcaat ttcctcaaaa tcaatcgcga agtcactatc
    12421 cagacggtat tccgctatgc caatcgttat ccggtcacga ttgaagcaat ttcttcaggg
    12481 cgattcgatg tgaaatcgat ggtgacgcat atttacgatt atcgggatgt acaacaggca
    12541 tttgaagagt cagttaacaa caaacgcgac attattaaag gcgttattaa gattagcgat
    12601 taattaatac gggagaacag aacatgctcg cagatatcag atattgggaa aacgatgcca
    12661 caaataaacg ttatgctatt gcccatttta atgtatggaa tgcagaaatg ttgatgggcg
    12721 ttatcgacgc agccgaagaa gcgaaatcac cggttattat ttcttttggc acaggttttg
    12781 tcggcaacac ctcatttgaa gatttctctc acatgatggt atctatggca caaaaagcaa
    12841 cggtgccggt aataactcat tgggatcatg gtcggagtat ggagattatt cataacgcct
    12901 ggactcatgg tatgaattca ttaatgcgtg atgcttccgc atttgatttc gaagaaaata
    12961 ttcgtttaac caaagaggct gttgatttct tccatccgct gggtattccg gtagaggcgg
    13021 aattagggca tgtcggtaat gaaaccgttt atgaggaagc gctggcgggc tatcactata
    13081 ccgatcctga ccaggcggct gaatttgttg aacgtacggg ctgtgactca ttggctgtcg
    13141 ccatcggcaa ccagcatggg gtttatacgt cagagccaca attgaacttt gaggtcgtca
    13201 aacgcgtacg cgatgcagtg tctgttccgc tggttttgca tggcgcatcg gggatcagtg
    13261 atgccgacat taaaactgca atttcgttgg gtatcgcgaa aatcaacatc catacggagc
    13321 tctgtcaggc cgcaatggtc gcagtgaaag aaaaccagga tcagcctttc ctgcatttag
    13381 aacgtgaagt acgtaaagct gtaaaagaac gggcattgga aaaaattaaa ctgttcggtt
    13441 ctgatggcaa agcggaataa tgatatggat aatctcgacg ttatttgtat aggtgccgct
    13501 attgttgata ttccattgca accggtcagt aaaaatatct ttgatgtgga ttcttatcct
    13561 cttgaaagaa tcgcaatgac caccgggggt gatgcaataa atgaagcaac aattatttct
    13621 cgtctgggcc atcgtacagc attaatgagc cgtattggta aagatgccgc agggcagttt
    13681 attctcgacc attgccgcaa agagaatatt gatattcaaa gcctgaagca ggacgtcaac
    13741 atagatacct ctattaatgt tggactggtg acggaggatg gcgagcggac gtttgtcaca
    13801 aaccgtaacg gcagtctgtg gaaattgaat attgacgatg ttgattttgc gcggttttcc
    13861 caggcgaaat tattatcact ggccagtatt ttcaacagtc cactattgga tggtaaagcg
    13921 ctaacagaaa tttttacgca agctaaagcc cggcagatga atatctgtgc cgatatgatt
    13981 aaaccgcggt tgaatgaaac gctggatgat atttgcgaag cattgagtta tgtcaattat
    14041 gccgaggcaa aattactcac cgggaaagag acactggatg aaattgctga cagctttctt
    14101 gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag acggctgctt tatcaagcgt
    14161 ggtgacatga cgatgaaggt gccggcggtc gcaggaataa ccgccatcga caccattggc
    14221 gcgggcgata actttgcttc aggttttatt gcggcactgt tagaaggaaa aaatctgcgt
    14281 gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg ttctaagcgt cggtgccacc
    14341 accggcgtaa aaaacagaaa gctggtcgaa caattgctgg aagaatacga aggataatga
    14401 aggcaaatga aaaagatacc tttaggcaca acggatatta cgctttcgcg aatggggttg
    14461 gggacatggg ccattggcgg cggtcctgca tggaatggcg atctcgatcg gcaaatatgt
    14521 attgatacga ttcttgaagc ccatcgttgc ggcattaacc tgattgatac tgcaccagga
    14581 tataactttg gcaatagtga agttatcgtc ggtcaggcgt taaaaaaact gccccgtgaa
    14641 caggttgtag tagaaaccaa atgcggcatt gtctgggaac gaaaaggaag tttattcaac
    14701 aaagttggcg atcggcagtt gtataaaaac ctttccccgg aatctatccg cgaagaggta
    14761 gaagccagct tgcaacgtct gggttttgat tacatcgata tctacatgac gcactggcag
    14821 tcggtgccgc cattttatac gccgatcgct gaaactgtcg cagtgcttaa tgagttaaag
    14881 gctgaaggga aaattcgcgc tataggcgct gctaacgtcg atgctgacca tatccgcgag
    14941 tatctgcaat atggtgaact ggatattatt caggcgaaat acagtatcct cgaccgggca
    15001 atggaaaacg aactgctgcc gctatgtcgt gataatggca ttgtggttca ggtttattcc
    15061 ccgctagagc agggattgtt gaccggcacc atcactcgtg attacgttcc gggcggcgct
    15121 cgggcaaata aagtctggtt ccagcgtgaa aacatgctga aagtgattga tatgcttgaa
    15181 cagtggcagc cactttgtgc tcgttatcag tgcacaattc ccactctggc actggcgtgg
    15241 atattaaaac agagtgattt aatctccatt cttagtgggg ctactgcacc ggaacaggta
    15301 cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg cagacgcaac attgatgagg
    15361 gaaatggcag aggtcttggg gcgttaaata ctcaacacga ttcacctctg tttctgtgaa
    15421 aactgtgatg aaaatctgtg attcaaacag gttattttga aagtaaacat cggttatgcg
    15481 ataatcgcgc taatgtgatg taaaaagata caggggtgta atcgtggcgg caaaagacag
    15541 gattcaggca attaagcaaa tggttgccaa cgataaaaag gtgacagtct caaatttgag
    15601 tgggattttt caggtaaccg aagaaaccat tcgccgcgat cttgagaagc tggaagatga
    15661 aggctttttg accagaacct atggtggtgc tgttttaaat acagcgatgt tgacggagaa
    15721 tatccatttt tataagcgcg cttcatcgtt ttatgaagag aagcagctta ttgcacgcaa
    15781 ggcactaccc tttatcgaca ataaaaccac catggcagcc gattccagta gtaccgtaat
    15841 ggaattgctc aaattgttac aggaccgtag tgacctgacg ttgctaacca actcggcgga
    15901 agcgattcat gttctggctc agtcagaaat taaagtcgtt tcaacgggtg gggaactaaa
    15961 caaaaataca ctttcactgc aaggaagaat tactaaagag atcatcagcc gctatcatgt
    16021 cgatatcatg gtaatgagtt gtaaaggtct tgatattaac agcggcgcgc tggactctaa
    16081 tgaagcggaa gctgaaatca aaaagacaat gatccgtcag gcgacagaag ttgcgttatt
    16141 ggttgatcac tctaagtttg atcgcaaagc ttttgtccag ttagccgact ttagtcatat
    16201 taattacata ataactgata aatcaccggg tgcagagtgg atagcatttt gcaaagacaa
    16261 taatatccag ctggtgtggt aatttaagca cgcgttgtca atattatttg ttcctgcgcc
    16321 tggaaataaa atgatgataa agatgcggca agtgaatttg ccgcaattgc tacctttttt
    16381 agcaaatccg aggcacccaa tggaacaata tgatcaaatt ggcgcaagac tggaccgctt
    16441 gcctttggcc cggtttcatt atcgtatatt tggtattata agctttagtc tgttattaac
    16501 a
//
LOCUS       DAZKFZ010000094        16364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000094 DAZKFZ010000000
VERSION     DAZKFZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.094"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            348..1247
                     /gene="yegS"
                     /locus_tag="ACHOQW_002721"
     CDS             348..1247
                     /gene="yegS"
                     /locus_tag="ACHOQW_002721"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP9573777.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1329..2102)
                     /locus_tag="ACHOQW_002722"
     CDS             complement(1329..2102)
                     /locus_tag="ACHOQW_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9573778.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(2208..3248)
                     /gene="gatD"
                     /locus_tag="ACHOQW_002723"
     CDS             complement(2208..3248)
                     /gene="gatD"
                     /locus_tag="ACHOQW_002723"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9573779.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3296..4651)
                     /locus_tag="ACHOQW_002724"
     CDS             complement(3296..4651)
                     /locus_tag="ACHOQW_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9573780.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4655..4939)
                     /gene="gatB"
                     /locus_tag="ACHOQW_002725"
     CDS             complement(4655..4939)
                     /gene="gatB"
                     /locus_tag="ACHOQW_002725"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP9573781.1"
                     /translation="MKRKIIVACGGEVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4970..5422)
                     /gene="gatA"
                     /locus_tag="ACHOQW_002726"
     CDS             complement(4970..5422)
                     /gene="gatA"
                     /locus_tag="ACHOQW_002726"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP9573782.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5432..6694)
                     /gene="gatZ"
                     /locus_tag="ACHOQW_002727"
     CDS             complement(5432..6694)
                     /gene="gatZ"
                     /locus_tag="ACHOQW_002727"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP9573783.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6723..7577)
                     /gene="gatY"
                     /locus_tag="ACHOQW_002728"
     CDS             complement(6723..7577)
                     /gene="gatY"
                     /locus_tag="ACHOQW_002728"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP9573784.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7876..8928)
                     /gene="fbaB"
                     /locus_tag="ACHOQW_002729"
     CDS             complement(7876..8928)
                     /gene="fbaB"
                     /locus_tag="ACHOQW_002729"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP9573785.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9185..10462
                     /locus_tag="ACHOQW_002730"
     CDS             9185..10462
                     /locus_tag="ACHOQW_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP9573786.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10459..11463
                     /locus_tag="ACHOQW_002731"
     CDS             10459..11463
                     /locus_tag="ACHOQW_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9573787.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            11460..12425
                     /locus_tag="ACHOQW_002732"
     CDS             11460..12425
                     /locus_tag="ACHOQW_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9573788.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12399..13145)
                     /locus_tag="ACHOQW_002733"
     CDS             complement(12399..13145)
                     /locus_tag="ACHOQW_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9573789.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13197..14015)
                     /locus_tag="ACHOQW_002734"
     CDS             complement(13197..14015)
                     /locus_tag="ACHOQW_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP9573790.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(14080..14880)
                     /gene="thiD"
                     /locus_tag="ACHOQW_002735"
     CDS             complement(14080..14880)
                     /gene="thiD"
                     /locus_tag="ACHOQW_002735"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9573791.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14877..15665)
                     /gene="thiM"
                     /locus_tag="ACHOQW_002736"
     CDS             complement(14877..15665)
                     /gene="thiM"
                     /locus_tag="ACHOQW_002736"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP9573792.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15700..15796)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15999..16238
                     /locus_tag="ACHOQW_002737"
     CDS             15999..16238
                     /locus_tag="ACHOQW_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFP9573793.1"
                     /translation="MTETQIYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKE
                     FCEGVGLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT         4085 a         4237 c         3934 g         4108 t
ORIGIN      
        1 atgattatta taattgaata ggattataat aaatgttctg taaaacattt cctacataag
       61 taggaattac ggacatggag gcccttccgg gtaactccat ggaggcttta atatattata
      121 ttgaagaagc cactgtttag ttgaatatta ggtatatgct cttttttgaa atttatcggt
      181 ggctgccgtt agtattcgct gtccccattg caagctcctg gtggtaacca ctgaatcctc
      241 catacttgaa ctgactattt atcctccgac tttcatcctg ttctgactcc accttttctt
      301 ttctgctcta cactatctat agaccaatca taaaggcaca tacgatcatg gcagaatttc
      361 ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg cgcgaagcaa
      421 ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg gagaaaggcg
      481 atgccgcacg atatgtagag gaggcccgga agttgggcgt cgcaacggtg attgccggtg
      541 gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag ggggatgaca
      601 tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc agtgtaggga
      661 ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaacgcc attgcgatag
      721 atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc ggatttggga
      781 cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc gtctcttaca
      841 tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa atccgcggtg
      901 aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg cgtcaggccg
      961 gtggcggtca acaattgtgc ccgaacgcgt taattaacga tggcttgctg caactgcgca
     1021 tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata
     1081 acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca
     1141 cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt ccatattgaa atacttccgg
     1201 cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc
     1261 tcttgaacca gggagaatcc gtaatgccgg atgcggtgta aacaccttat ccggcataca
     1321 gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccag tttatccaca
     1381 tctgacacgg catcatcggt aataataatg tcaatttttt cgattggcag tacctgatta
     1441 aaaccgcgac gattaaattt cgatgaatcg agaacggcaa tcactttatt tgcggcggtg
     1501 accatcgccc cgctaatcga ataaccttca ttaaaggtcg taataccatt aaccgcatca
     1561 ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaatga acgctcggca
     1621 atggaaccat gcatcgagcg cgttttatgg cggaccgtgc caccacagac gaccagagta
     1681 atgtctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat caccgtgata
     1741 tttttggcgg tcattaatcc ttcggcgata agcattgtcg tacttccgct gtcgagaata
     1801 acggtcatcc cttcgtggat cattttgact gccgcctgcg ctattctgtt ttttggcgcg
     1861 ctggcaagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattacca
     1921 gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag
     1981 cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca
     2041 agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc
     2101 atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat
     2161 gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca gggaatgagc
     2221 aacactttgc ccggcatagc attacgagcg atgtcacgca ccgcctgggc gaagctttca
     2281 aagcttccac ggtgagcgat taatggctcc aggcttaact tacgttctgt cagcaaccgg
     2341 ctcgccgttt cccactcctg ccccggccaa gggctggagt agttcatcca actgccgata
     2401 accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga
     2461 tgcaacgtgc ccaccagtgc cagttgggca tgaggaccgg caatctctac cgccagttcg
     2521 acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaagcgcag ttcgcgtaaa
     2581 acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgcaccgaaa
     2641 gattttgcca gtgccagttt ttctgagcta atgtcgatag ccgtcacact ctttgctccc
     2701 agcgcgacag cgcactgaat agccagcagg ccaatggttc cggcaccaat aataataacg
     2761 tttttattct cacaaccttg cgctaaatga aaagcatgca ggccaacggt aatcggctca
     2821 ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt ttttcgctta
     2881 acgacaatat attcggcaaa tccaccatca cgccgtgagc caataaaatc atattttgcg
     2941 cactgggaat aaaacccttt caaacactct ggacaagtaa aacagggtaa taacggcaca
     3001 caggcaaccg catcgccagg gtgtaaatca tcaacaccgg atcctaccgc atcaatatag
     3061 ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg
     3121 ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgatgt
     3181 ttaatttcag gaatgacgct ttctgcaacg cgcacgatac catcagtatc attcaccact
     3241 gatttcataa aaactcctaa ttgttaaggg ggataacctc cccctcaggt aaaaattatt
     3301 ctgcgagaac gactttctct tgtttaataa agccacgcgc tctacgccag gtcatgaaaa
     3361 taccggtaag ataaattgcg ccgataataa tgaaaccggg aatattttgc ggggagaaaa
     3421 cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc accatacccc
     3481 cggcttttaa cgccccagca ttagccgcca gttgggtgtg caaaccaata gtttgcgtcg
     3541 cgatccacag ggtgatgctc ataatgatga cacccgagat taaggtgcgg aacagatttc
     3601 cacgatgcac cgcgaccgcc atcgccacga agaagccgat agtggcaaga tcgccaaacg
     3661 gcagtacctg attaccagga acacaaacag caattaaaat ggtgagcgga ataaaaatca
     3721 ggcttgccga taccaccgcc gtatgcccca gcagtaatgc gggatccagg ccaatcagga
     3781 actcctgacc gccgaacttc gcctgtaaac gactacgggc ctgcttagcg atgggcgtta
     3841 aaccatccat gatgggttta atcacccgtg gcattagcag catcaccgcc gccgttttta
     3901 ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata atcagcccca
     3961 tcacaaaacc cacggtgaca ggctcgccaa acgggccaaa tttgcgctga atatcgtcgg
     4021 cgctaaattt aatacggtta acgcctggga ttttctcgat gatagcatcg accagcaccg
     4081 caatcggccc catatacgcc gacgtaccgt gcggaatagc aatgccttcc agctcaaaga
     4141 aatttcgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta
     4201 ccacacctgc catccctatc atccatgaac cggttgccag atgcagcaac gcgccggtga
     4261 aggtcatatg ccagatattc cagatatcaa catttaccac ccgcgtcata cgggtcagta
     4321 acatcgccac gttaaccaga atcgcaatcg gaatcgccac cagcgcaatt tgcgacgccc
     4381 aggtcattgg tgaagagccc ggccagccga catcgaccac atgcagattc aggtcgaaat
     4441 tttccgccat cgctttcgcc gccgggccaa tggaatccag cattaagcca atcacaaggc
     4501 caatgccaac aaagccaatc ccgatatgca gacccgcttt aaagcaatcg cctgccttca
     4561 tgcctaaaat tttagaaaaa ataatgatga caatcggcaa catcaccgta gggccgagat
     4621 cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata gtcagaattt
     4681 tattttgtaa tgcttcgata ccgacaccag aaacaaaagg catgccgtga actaacggaa
     4741 tatcgccaaa actacgatcc accctggcag tggtgcatat caaatgcacg ccatccatat
     4801 aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta tgactctgac
     4861 acaactcttt aatttcttcc gccgccatcg tagaggtcgc aacctcgcct ccgcaagcga
     4921 caataatctt gcgtttcata aatagtcctt tttattgtta cagagggatt tattcgtctg
     4981 aatctaaaac atactttgtg aagtattcct ttaactgagt ttcaggaaga gtgattagtg
     5041 tctcaacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct
     5101 gctgcggatt ttccacaatc aacgcaataa ccaacgatac cgccacgtca ttatcatcat
     5161 ccgcttgctg aaaatgaact ttatttgttg gccttaacag ataaatggct gacgacttag
     5221 catgaatcgc ctcacaatgc ggtattgcaa tggcgtgctg ctcaagcatt atcccggtag
     5281 ggaattctgc ttctctggca attaatgcct gtggccaggt atcatgaacc acacctttgg
     5341 cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa gaaattccgc
     5401 tacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag cgataggcgc
     5461 gcaaaacatc ataaatttta tccataatca gctgatgcgg tattgctgat aattccccgg
     5521 actgaatgcg ttcaaattgt ttgggaagat actgactaat catgcccagt gggatgtcca
     5581 cgccttgcag attcaccatc atcgtttcaa cgctattttt aatccgacta tgcggccagt
     5641 aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg ttaaaacccg
     5701 tgcgataata ttttttccag tattgcggtt cgtcgagcat cacttcttca attaccgcca
     5761 ggcaaccgct gcgattttcg ggggcgataa gttcctgctc aatttgcgcc agcgcaaata
     5821 tcgcctcacg taaagcaaag gttaatgcgg gaccgacttt caatattgca aagtgatcgc
     5881 ggactaattc ccaataagcc gtccgggtct ggtaatcggt agaatgtgct tcataaacca
     5941 ttcgggtatt ttctatccat tgcgccagcg cctgcgcttc ctgcggctga taatggataa
     6001 tattgctgtg atcaaattcc acacccggct gcaccacgat ggcaatcacg cgtgttaatg
     6061 cctctgtcag cccacgggca ataaaggcct tttgatgcgt acgtaaagta ttggcggcat
     6121 cttcaacatg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc ggaaccggaa
     6181 cttcggtgcc aatgacatag ctcagttgct cacgctggca atctgtcgcc acactttccg
     6241 cagcaaagca aagcacagca gctcgttccg caaccgtttc tggtgctaac ggtatgggat
     6301 cccccgcgca ggacattgac gcatcaagat gaattttact gaagccagca cgaacatatg
     6361 cctttaccag ctcgacggat ttttccatcg ccgcatccac attttcttgc tgccagcagt
     6421 ttggccccag atggtcaccg ccgagaataa tacgctcgcg tgcaaatccg actttatcgg
     6481 caatcgcaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt
     6541 gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg cgatcaaatg
     6601 ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg ccgatatgtt
     6661 caccagcttt atgccgggca attaacgttt tcatgttttt tccttgttaa atggcaggtg
     6721 cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac atcgcgcatt
     6781 gcggatttag ccgactgcaa ataatcccgg ggatcggtcg cttcggggtg ctcggtcagg
     6841 taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt gattttgcat
     6901 atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc gccatgcagc
     6961 accagcggta agttcaccca ctggcgaatg ttctccagtc gagaaaaatc aagcgccggt
     7021 gcgctggcat acataccatg agccgtgccg atggcgaccg ccagggaatc aattccggtt
     7081 gcctcggcaa attcacgcgc ctgagcgggg ttggtataaa aagcatcggc ttcattgact
     7141 tgcacatcat cttcctggcc gccaagttgc cccagctccg cttcgacgct gacatcaaag
     7201 cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc
     7261 gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat
     7321 ttcgtgtgat ggtcgagatg aatcgccagt ggatggtgat aatgcttcgc catcgcgctg
     7381 accagcgcca acagattttc tgtaccagca tgagtaaatg tgccaggcgt tccggcgatg
     7441 atgaccggcg catgcaggtt ggcagcggtt tctaccacca cttgcatcgt ttcgagattg
     7501 tgaatattga atgccggaac cgcgtaaccg ccgcgctgcg cgttgttcag catctgcttt
     7561 gtcgatacca cgtacatttt cacatcatgt cgattgcttt cgatttcaaa acataatgaa
     7621 atcatttgtt tttaaatgtc gagataacga tcacaaaaac gacaatatga aaattaatag
     7681 aggaatgaaa ggcaaaaaaa acggcctccc ggagggaagc cgcaacaaag tgcgcgtggt
     7741 tttatgcagg atgcggcgta aacatatgtg gcagcgctgc gtaggcatga taagacgctt
     7801 cagcgtcgca tcaggcatag gttgccggat gcggcgtaaa cgccttatcc gtcctacggg
     7861 gtagtgccgt tcgtatcagg cgatagtaat tttgctatcg agataaacgt cctgcacggc
     7921 gttaatcagt ttcacgccgt cagccatcga tttcttgaac gccttacgtc caagaatcag
     7981 ccccattccg ccagcgcgtt tgttgataac cgcagtacgc actgcatcac tgaggtcagt
     8041 ttcaccgcct gcagcaccac cggagtttat caacccggcc cggcccatat agcagttagc
     8101 taactgataa cgcaccagat caatcgggtt ttcgctggtt aacttgctgt acacgcgatc
     8161 gtcggtataa ccgtaattaa ttgctttata gccgccgtta ttttccgcca ttttttgttt
     8221 gacgatatct gcacctatgg ttgccgccag atggttcgcc tgaccagtca ggtcggcgga
     8281 gacatggtaa tcaacgccat ctttcttaaa ggcggagtta cgcaaatagg cccacagcac
     8341 tgtcaccatg cccagctcgt gcgcacgttc aaaagccgca gaaatttctt caatctggcg
     8401 acgtgactct tccgagccaa aatagatagt cgcaccaacc gccaccgcgc ccatgttgaa
     8461 ggcctgctcc acgctggcat acagcgtttg atcgtaggta ttcgggtaac ttagcgtctc
     8521 gttgtgatta agttttacga gaaatggaat gcgatgcgca tagcgccgcg ataccgacgc
     8581 caacacgccg taagttgatg ccacacagtt acagcccgct tcgatcgcca gttcaacaat
     8641 gtttttcggg tcaaagtaga gcgggttagc agcaaatgaa gctccggcag agtgctcaac
     8701 gccctggtca accggcagaa tagaaagata tcctgtgcca gccagacgcc cggtgttgta
     8761 caacgtctgc atattacgta acaccgctgg cgggcgatta ttgtcaatca tcacgcggtc
     8821 tacgtagtca tgtccgggga gataaagctg gtcagaagga atagtcatac aacggtgctg
     8881 taaaaggttg tcggcgtctt tgccaagcaa ctgcgcaata tctgtcatta ctatgctccc
     8941 gtaaattccg attggatatc ggctatggat tgtcctggcc cgccttttgc gggcaatcat
     9001 aatcctggtc gttacgggcg agtttttcca tcttttggat gttttttcag cgttttctgt
     9061 tggctcgatt catcagaaaa aatgttagcg cggtcaagtt ttcaccgcaa aggtatttaa
     9121 aaggtattat taagtggtat tgtcatcgcg taccttacat tacctgtcat gaaggaatta
     9181 aaagatgaaa acaacaacaa agctgtcgtt catgatgttt gttgaatggt ttatctgggg
     9241 cgcgtggttt gtgccattgt ggctgtggtt aagtaaaagc ggttttagtg ccggagaaat
     9301 tggctggtcg tatgcctgca cagccattgc ggcgatcctg tcgccaattc tggttggctc
     9361 catcactgac cgctttttct cggcgcagaa agtgctggcg gtattgatgt tcactggtgc
     9421 ggcgctgatg tatttcgctg cgcaacagac cacttttgcc gggttcttcc cgttactgct
     9481 ggcctactcg ctaacctata tgccgaccat tgcgctgact aacagcatcg cttttgccaa
     9541 cgtgccggat gtggagcgtg atttcccgcg cattcgtgtg atgggcacta tcggctggat
     9601 tgcctctggt ctggcatgtg gtttcttgcc gcaaatgctg gggtatgccg atatctcacc
     9661 gactaacatc ccgctgctga ttaccgccgg aagttctgct ctgctcggtg tgtttgcgtt
     9721 tttcctgccc gacacgccgc caaaaagcac cggcaaaatg gacattaaag tcatgctcgg
     9781 cctggatgcg ctgatcctgc tgcgcgataa aaacttcctc gtctttttct tctgttcatt
     9841 cctgtttgcg atgccactgg cgttctatta catctttgcc aacggttatc tgaccgaagt
     9901 tggcatgaaa aacgccaccg gctggatgac gctcggccag ttctctgaaa tattctttat
     9961 gctggcattg ccgtttttca ccaaacgctt tggtatcaaa aaggtattat tgcttggtct
    10021 ggtcaccgct gcgatccgct atggcttctt tatttacggt agtgcggatg aatatttcac
    10081 ctacgcgtta ctgttcctcg gcattttgct gcacggcgta agttacgatt tttactacgt
    10141 taccgcttac atctatgtcg ataaaaaagc ccccgtgcat atgcgtaccg ctgcgcaggg
    10201 actgatcacg ctctgctgcc agggcttcgg cagtttgctc ggctatcgtc ttggcggtgt
    10261 gatgatggaa aagatgttcg cttatcagga accggtaaac ggactgactt tcaactggtc
    10321 cgggatgtgg acttttggcg cggtgatgat tgccattatc gccgtgctgt tcatgatctt
    10381 tttccgcgaa tccgacaacg aaattacggc tatcaaggtc gatgatcgcg atattgcgtt
    10441 gacacaaggg gaagttaaat gaaaacagaa cgtattctcg gtgctcttta tgggcaggcg
    10501 ttaggggatg cgatggggat gccctccgag ctttggccac gcagccgcgt taaagcacac
    10561 tttggctgga ttgaccgttt tctgcctgga ccaaaggaga acaacgcggc ctgttatttt
    10621 aaccgcgccg aattcaccga cgatacctcg atggcgctgt gtctggcgga tgcgttactg
    10681 gaacgtgaag gcaagatcga cccgaatctg attgggcgta atattctcga ctgggcgctg
    10741 cgtttcgacg cctttaacaa aaacgtacta ggtccgacct cgaagattgc gcttaacgcc
    10801 attcgcgacg gtaaacccgt tgctgaactg gaaaacaacg gcgtgaccaa cggcgcagcg
    10861 atgcgcgtct cgccattagg ttgtttgctt ccggcgcgtg atgttgattc ctttattaat
    10921 gatgtggcgc tggcgtcgag cccgacccat aaatccgatc tggcggttgc gggcgcggta
    10981 gtcatcgcat gggcgatttc tcgtgctatt gacggagaaa gctggtcagc gattgttgat
    11041 tcattgcctt caattgcgcg acatgcacag caaaaacgca ccaccacgtt cagcgcctca
    11101 ctggcagcgc ggctggagat tgcgctgaaa attgtgcgca atgccgacgg cactgaatcc
    11161 gccagcgaac agctttacca ggtcgttggc gcaggtacca gcactattga gtccgttccg
    11221 tgcgccattg cgctggttga actggcacaa accgacccga atcgctgcgc cgtcctgtgc
    11281 gctaaccttg gcggcgatac agacaccatc ggtgctatgg cgacggcaat ctgcggcgcg
    11341 ttgcatggca ttaacgctat cgatcctgcg ttaaaggcgg agttggatgc ggtaaatcag
    11401 cttgatttca accgctatgc cacagcgctg gcgaaatatc gtcaacaacg ggaggcggca
    11461 tgagcggcgc tcgattacac acgctgctgc ctgaattaac cacgcgtcag ccggttatgg
    11521 tgattggcgc ggcagtcatt gatgtgatcg ccgacgctta tgccctcccc tggcgtgggt
    11581 gcgatatcga actgaaacag cagagcgtta acgttggcgg ctgcgcactg aatattgccg
    11641 tggcgttaaa gcgcctcggc atcgaagcgg gtaacgcctt gccgctcggt cagggcgtgt
    11701 gggcggagat tattcgcaac cggatggcaa aagagggctt aatcagcctg atcgataacg
    11761 ccgaaggtga taacggctgg tgtctggcgc tggttgagcc ggatggcgaa cgcactttta
    11821 tgtcattcag cggtgtggaa aatcagtgga atcgccagtg gctggcgcga ttaaccgttg
    11881 cgcctggcag cctgctctat ttttccggtt atcaactggc ctcgccctgc ggcgaattat
    11941 tagtggaatg gctggaaaag ctgcaagacg tgacgccgtt tatcgatttt ggcccacgta
    12001 ttggcgatat cccggaggca ttactggcgc ggatcatggc ctgtcgacct ttagtgtcgc
    12061 tcaatcgtca ggaggctgag attgccgccg aacgttttgc tttatccgca gagataacaa
    12121 cacttggcaa gcaatggcag gagaaatttg ccacgccgtt gatcattcgt ctcgataaag
    12181 agggcgcatg gtatttcagc aacgacgctt ctggctgcat tccggcattt cccacgcaag
    12241 ttgtagacac cattggggcg ggcgacagtc atgcaggtgg cgtacttgcc gggttggcct
    12301 ctggtctgcc actggcggat gccgtattac tgggcaatgc agtggcgtcg tgggttgtcg
    12361 ggcatcgggg cggtgattgt gcgccaacgc gcgaggaact actcctcgca cacaaaaacg
    12421 tatagatcgc tgcgacagtg gctaatgctg tactcaatag gccgttgctg ttggtcaagc
    12481 gccacttgct tgatcaccag caccggtatt ttgctatcta actgaatatg tgactgaaac
    12541 tcggcatctg gcatccgggc gctaacgcgg gaacgtgtac gttgtgggta aatgtgctgg
    12601 ctgcggaaat agtcataaag agaaattccg atggcatcaa catcatgaat taaatgcgcc
    12661 ggaacccacg attcctcaat cgataccgct tcttcatcga catagcgaat acgcttaagc
    12721 aaaaagacat cacttcccgc ttcgaccgcc agctgctgcg cgacttcttc gggacatttc
    12781 acgacacgct tgttaaccca tagcgtatcg ggctttttcc cgcgcaatac cacctgctga
    12841 gaaaaacctc gcgcttcttt caacgagtat tcgaagatgt tgttgatctg cgtgccgtaa
    12901 ccgcgcgaac gcgtcaccac accctcttct tccagcgcct gcatcgcctt gcgcaccgta
    12961 atgcgtgaaa cgccggttaa ctgacttaaa tcacgctcac cgggcaaaat attgccatgc
    13021 tccagcaccc cgctgcgcac ggcatttttt accgtttcgg caaacttaat ataaagcggc
    13081 gtgttatccg ccgctaaaat acgctcgttc agttgggcaa tcagctgggt atgcgcttgt
    13141 tccatttgtc tttttcctgc cagggagtct ggtgggagta tatcactgct tcttatttaa
    13201 ggcgtttctt taatcccatc aacgaatgcc tgcaactcct cctctgtgcc attaaacaca
    13261 ttaaaatcca ccgaaccatt aatgccatct acctgcccac ggtcggaatg ctgccagaaa
    13321 cgccaggcca tgccgtcatt gtccgggcga cgttgatagt agtgagccac ccaccacgga
    13381 tattcattga aatagcccgc cagattggtg tgataaaaaa cggctcctga gtaaataacc
    13441 ggcttttttc ccgtactttt ttcgaccatt tttagccact gacttacccg cttgcgtaat
    13501 tcttttgccg ataattttcc ccgttcttct acgtccagca cggcagggaa atcgccttgt
    13561 gagaaatcca ccgtttgcag aaataatcgc gcctgaacag gcgctgctac cgacggggag
    13621 aaataatgat acgccccgcg cagcaggcca ttttcgcggc ttagttgcca gttacgcgaa
    13681 aaataggggt ccaccagctt ttcgccttcc gtcgccttga taaaggcaaa ttgtaagcgg
    13741 ataccgttat cgcgcatttt tgccacccgc tgccagtcga tccgctcctg ccagcgcgaa
    13801 acatctattc cgtgaatggt gtaactggca ggaatatgaa tagcgaaaga tttaaccggg
    13861 cgataaccgt agaagtggat gtagtctgta atctgaactg ctgtcgaata gaaaaaattc
    13921 accgtctgac gcggataaat tgcaacgatg gaaattagcc caagagcgca taatagagcg
    13981 gtgagttttt ttcgactggt aattcttagc tgcattggtt tcatccctga atgtcagtgc
    14041 caaaggctga caataaccaa agcagctata gtacggtgct caccaccagg cgtggaagtg
    14101 gtgaaccgga ccaataccgt gaccaacttc cagcgtgtcg gcctgggcta acgccgatga
    14161 aagccagctt tttgcctcct gtacggtgtc agcccagttt gtatggcgcg ggcgtagtgc
    14221 agccaacgcc gcagagagtg tacaaccagt gccgtgggtg tttttggtca taatgcgcgg
    14281 tgcggtaaac cgttgttcac cctcgcgggt aaacagccag tccgggcttt gctcatcatc
    14341 cagatggcca cctttcatta gcactgcgcc acagcccatc gccaacagcg ctcgcccttg
    14401 ttccagcatt tcctgttcgg tgcgcgcgtg tggcgcgtcg agcaaggcag cagcttcggg
    14461 caagtttggc gttattaatg aaacctgtgg caataatcga ctgcgtagcg tggcaactgc
    14521 cgaaggtgaa agcagtgggt cgccactttt tgccagcata acggtgtcga gtaccacgtt
    14581 ttggatctga taacgttgca accgttctgc caccgcttca acaatatcgg tttccgccaa
    14641 cataccgatt ttagtggtat cgattcgcac atcgctgaac accgaatcga gctgggcggc
    14701 gacaaaatca ggctcaatgc gatacaccga ctgtacgcca cgggtatttt gcgccaccag
    14761 tgcagtaata actgagcaac cataagcgcc aagtgccgag aaggttttaa gatcggcctg
    14821 aatccccgca ccaccactcg gatcagtacc ggcaatcgtc agagcgttaa ttcgtttcat
    14881 gcctgcacct cctgcgtcaa ttgccagagc gcatcaagga aatgtggaac aaaactgcct
    14941 ggcccctcgc ttctggcgac tgcgcgttct ccggcttgtt tcatccagtg acaggcagat
    15001 gcgacatttt ccagcatatc gcctggtaac gcacaacagg cagcgacaac cgccgataat
    15061 gcacagccag ttcctaccac tttggtcatt aacggatcgc caccgtgaat gccaacggcg
    15121 cgatggccat cggtaacata atccacctcg ccagtgacca cgacgattgc gccagtttcc
    15181 cgtgccagtg tttgtgcagc gggtatcgcg ttaactgcgg cgtcagtggt atccactccc
    15241 cgtccaccat tagcaacgcc agctaatgcc atgatttccg aagcattacc acgtatcgct
    15301 gccggtttaa aagataaaag ttcatgacaa aaatggcggc gataatcgag cgcaccaacc
    15361 gctactggat caagcgtcca gggtgtttga gagctttttg cttgctcaac ggcagcacgc
    15421 atcgcctgtg cgcgtggctg cgtcagtgtg ccaacgttaa tcaacaaggc actggcgata
    15481 gccgcaaact gactggcctc ttcggtttcg ataaccatcg ctggcgatgc accgagcgcc
    15541 agcaaggtat tggcagtaaa ggtttgcacc acatcgttgg tcatgcagtg cacaagaggg
    15601 gaatgttggt gaaaaaggtg taacgtgtgc gcagattgcg cggaactcag caggtcgact
    15661 tgcatagttt gctcctgcca taacgtgaag aagcaatgac ctggtggtcc gtgacttccc
    15721 tacgctggca ttatccagat caggtgatac gggtatttct cagccttcac gcagaagggc
    15781 accccgagtc gtttggttga atctctatat tttgtgccag cttaggggaa gaggcttgca
    15841 gatgcaagct cgcctccatt cattacgtct tcactgtgta ccccatatca gtaatgaaaa
    15901 agaaacaatt cacacccatc attcaggtgg taaaaactgt agactatctt aattagctaa
    15961 tttatattat ttatctaaat ttgataggtg agataaaaat gacagaaacc cagatctacg
    16021 agaacattaa gcaagccatt agcagcgcac cacgaaatag ccaaacgatg gaaatgcacc
    16081 tccagatgat caaatacgct gaccatctga aaaacgtcac ggcaaaagag ttttgcgaag
    16141 gtgtaggact taaagccagc tttgctacag agttcagcaa aatgagaaat cttaccgagc
    16201 gactcaaagc cgccggactt gataccacga agttataaca aacgaaatca tcaatatatt
    16261 actttcgttt tccagggttc cctgtatatg tacctatgat cataaaaacc agttaccacc
    16321 acgtagctga taccataata ttttcatttt tactttaaat aaat
//
LOCUS       DAZKFZ010000095        16294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000095 DAZKFZ010000000
VERSION     DAZKFZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            113..2809
                     /gene="stcE"
                     /locus_tag="ACHOQW_002738"
     CDS             113..2809
                     /gene="stcE"
                     /locus_tag="ACHOQW_002738"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFP9573794.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            2896..3771
                     /gene="gspC"
                     /locus_tag="ACHOQW_002739"
     CDS             2896..3771
                     /gene="gspC"
                     /locus_tag="ACHOQW_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9573795.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3772..5739
                     /gene="etpD"
                     /locus_tag="ACHOQW_002740"
     CDS             3772..5739
                     /gene="etpD"
                     /locus_tag="ACHOQW_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFP9573796.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5739..7244
                     /gene="gspE"
                     /locus_tag="ACHOQW_002741"
     CDS             5739..7244
                     /gene="gspE"
                     /locus_tag="ACHOQW_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9573797.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7246..8469
                     /gene="gspF"
                     /locus_tag="ACHOQW_002742"
     CDS             7246..8469
                     /gene="gspF"
                     /locus_tag="ACHOQW_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9573798.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8500..8934
                     /gene="gspG"
                     /locus_tag="ACHOQW_002743"
     CDS             8500..8934
                     /gene="gspG"
                     /locus_tag="ACHOQW_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9573799.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            8931..9485
                     /gene="gspH"
                     /locus_tag="ACHOQW_002744"
     CDS             8931..9485
                     /gene="gspH"
                     /locus_tag="ACHOQW_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9573800.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9482..9847
                     /gene="gspI"
                     /locus_tag="ACHOQW_002745"
     CDS             9482..9847
                     /gene="gspI"
                     /locus_tag="ACHOQW_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9573801.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9844..10443
                     /gene="gspJ"
                     /locus_tag="ACHOQW_002746"
     CDS             9844..10443
                     /gene="gspJ"
                     /locus_tag="ACHOQW_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9573802.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10440..11417
                     /gene="gspK"
                     /locus_tag="ACHOQW_002747"
     CDS             10440..11417
                     /gene="gspK"
                     /locus_tag="ACHOQW_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9573803.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11351..12628
                     /gene="gspL"
                     /locus_tag="ACHOQW_002748"
     CDS             11351..12628
                     /gene="gspL"
                     /locus_tag="ACHOQW_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9573804.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12615..13127
                     /gene="gspM"
                     /locus_tag="ACHOQW_002749"
     CDS             12615..13127
                     /gene="gspM"
                     /locus_tag="ACHOQW_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP9573805.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13248..14018
                     /locus_tag="ACHOQW_002750"
     CDS             13248..14018
                     /locus_tag="ACHOQW_002750"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9573806.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14110..14511
                     /gene="eptO"
                     /locus_tag="ACHOQW_002751"
                     /gene_synonym="gspS"
     CDS             14110..14511
                     /gene="eptO"
                     /locus_tag="ACHOQW_002751"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFP9573807.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            14690..15861
                     /locus_tag="ACHOQW_002752"
     CDS             join(14690..14939,14939..15861)
                     /locus_tag="ACHOQW_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9573808.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
BASE COUNT         4027 a         3587 c         4624 g         4056 t
ORIGIN      
        1 gtcaggatat atttacgaaa caggtgtaaa tatgttataa aaataaccaa cgactagtga
       61 ataagtcgct cctgaaaaaa taaaatatag aaatactgtt atatccggct gcatgaacac
      121 taaaatgaat gagagatgga gaacaccgat gaaattaaag tatctgtcat gtacgatcct
      181 tgcccctctg gcgattgggg tattttctgc aacagctgct gataataatt cagccattta
      241 tttcaatacc tcccagccta taaatgatct gcagggttcg ttggccgcag aggtgaaatt
      301 tgcacaaagc cagattttac ccgcccatcc taaagaaggg gatagtcaac cacatctgac
      361 cagcctgcgg aaaagtctgc tgcttgtccg tccggtgaaa gctgatgata aaacacctgt
      421 tcaggtggaa gcccgcgatg ataataataa aattctcggt acgttaaccc tttatcctcc
      481 ttcatcacta ccggatacaa tctaccatct ggatggtgtt ccggaaggtg gtatcgattt
      541 cacacctcat aatggaacga aaaagatcat taatacggtg gctgaagtaa acaaactcag
      601 tgatgccagc gggagttcta ttcatagcca tctaacaaat aatgcactgg tggagatcca
      661 tactgcaaat ggtcgttggg taagagacat ttatctgccg cagggacccg accttgaagg
      721 taagatggtt cgctttgttt cgtctgcagg ctatagttca acggtttttt atggtgatcg
      781 aaaagtcaca ctctcggtgg gtaacactct tctgttcaaa tatgtaaatg gtcagtggtt
      841 ccgctccggt gaactggaga ataatcgaat cacttatgct cagcatattt ggagtgctga
      901 actgcctgcg cactggatcg tgcctggttt aaacttggtg attaaacagg gcaatctgag
      961 cggtcgccta aatgatatca agattggagc accgggtgag ctgttgttgc atacaattga
     1021 tatcgggatg ttgaccactc cccgggatcg ctttgatttt gccaaagaca aagaagcaca
     1081 tagggaatat ttccagacca ttcctgtaag tcgtatgatt gttaataatt atgcgcctct
     1141 acacctaaag gaagttatgt taccaaccgg agagttattg acagatatgg atccaggaaa
     1201 tggtgggtgg catagtggta caatgcgtca aagaataggt aaagaattgg tttcgcatgg
     1261 cattgataat gctaactatg gtttaaatag taccgcaggc ttaggggaga atagtcatcc
     1321 atatgtagtt gcgcaattag cggcacataa tagccgcggt aattatgcta atggcatcca
     1381 ggttcatggt ggctccggag gtgggggaat tgttacttta gattccacat tggggaatga
     1441 gttcagtcat gaagttggtc ataattatgg tcttggtcat tatgtagatg gtttcaaggg
     1501 ttctgtacat cgtagtgcag aaaataacaa ctcaacttgg ggatgggatg gtgataaaaa
     1561 acggtttatt cctaactttt atccgtctca aacaaatgaa aagagttgtc tgaataatca
     1621 gtgtcaagaa ccgtttgatg gacacaaatt tggttttgac gccatggcgg gaggcagccc
     1681 tttctctgct gcaaaccgtt tcacaatgta tactccgaat tcatcggcta tcatccagcg
     1741 tttttttgaa aataaagctg tgttcgatag ccgttcctcc accggcttca gcaagtggaa
     1801 tgcagatacg caggaaatgg aaccgtatga acacaccatt gaccgtgcgg agcagattac
     1861 ggcttcagtc aatgagctaa gtgaaagcaa aatggctgag ctgatggcag agtacgctgt
     1921 cgtcaaagtg catatgtgga acggtaactg gacaagaaac atctatatcc ctacagcctc
     1981 cgcagataat agaggcagta tcctgaccat caaccatgag gccggttata atagttatct
     2041 gtttataaat ggtgacgaaa aggtcgtttc ccaggggtat aaaaagagct ttgtttccga
     2101 tggtcagttc tggaaagaac gtgatgtggt tgatactcgt gaagcgcgta agccagagca
     2161 gtttggtgtt cctgtgacga ccctggtggg gtattacgat ccggaaggca cgctgtcaag
     2221 ctacatctat cctgcgatgt atggtgccta tggcttcact tattccgatg atagtcagaa
     2281 tctatccgat aacgactgcc agctgcaggt ggatacgaaa gaagggcagt tgcgattcag
     2341 actggctaat caccgggcta acaacactgt aatgaataag ttccatatta acgtgccaac
     2401 agaaagtcag cccacacagg ccacattggt ttgcaataac aagatactgg ataccaaatc
     2461 gctcacacct gcgccagaag gacttaccta tactgtaaat gggcaggcac ttccagcaaa
     2521 agaaaacgag ggatgcatcg tgtccgtgaa ttcaggtaaa cgttactgtt tgccggttgg
     2581 tcaacggtca ggatatagcc ttcctgactg gattgttggg caggaagtct atgtcgacag
     2641 cggggctaaa gcgaaagtgc tgctttctga ctgggataac ctgtcctata acaggattgg
     2701 tgagtttgta ggtaatgtga acccagctga tatgaaaaaa gttaaagcct ggaacggaca
     2761 gtatttggac ttcagtaaac ctaggtcaat gagggttgta tataaataat cctgagacgg
     2821 tgcggccact tcggccgcat tagtaacaca ttcgtcccgt taatcaaaac ataaagtagc
     2881 gtccagcgtt ttattatgtt gttctttcta tcattccggg gtgaccgggg cttatttata
     2941 aaagatatcg tacttaaaat gctgacgcca aaccggctat tgtgcgtaat tttacttatt
     3001 gccggatatc agctggtgtc ggttatccat catttctggc tgactcaggc agcatcggtg
     3061 cccggcttat cccgtgttag cgcgccggaa acagcggtaa ctggtgatca aactgaagaa
     3121 cgttttgttt ttacgttatt cggcagggca tccccactat catcggaggg gagagcgcag
     3181 gaaacaatgc cttccctgtc agatgatctg ctttcagggg aggatcttga cgtgagaggt
     3241 atactttata gttcggttgc agagcattcc gttgccatat ttgcacataa taacagacag
     3301 ttcagtctga gcgtcggcga aaaagtaccc agctacgatg ctacgattag tgctattttt
     3361 agcgatcata ttgttattaa ctatcaggga aaaactgtat cactgcctct gcgatatgat
     3421 aataccgaaa aaaagaatgc atatgacaat aataatttaa cagttggaga cgtgataacc
     3481 caagataatt ttcgggtaga aagtgttttt gatattatga gcttttcagc cgttacggtt
     3541 aataatacat taagcggtta tcgcttgatt ccgggtaagc acagttcgtt attttataat
     3601 gctgggttgc atgataacga tctggccgta tcggttaatg gttcagaatt gcgtgatacc
     3661 agacaggcgc agcagataat gaagcaattg ccagaactta aagaaataaa aataaccgtc
     3721 gagcgtgatg gtcagttata tgatgcattt attgctgtag gagaaaactg attgttcagg
     3781 aaatggttga atgggcgttt gccggtactt gtgttcacta cagtaatttt gggggccatt
     3841 ccagggtggg gggctgaatt ttcggccaac tttaaagata cggatattca ggagttcata
     3901 aatactgtca gtaaaaattt acacaaaacg gtaataatta atcctgacgt gcagggaacc
     3961 atcactgtac gcagctacga tatgctgaac gaggaacaat attatcagtt ctttctcagt
     4021 gtgctggacg tttatggttt tgctgtggtc gatatgcaca acggtatact gaaagtagtg
     4081 cgctcaaaag atgccaaaac gtcggcggtg ccggtagcta gtgatgtcag tcccgggact
     4141 ggtgatgagg ttgttacccg ggtggtcccc gtaagtaacg tggcagccag agatctggcg
     4201 cctttgctgc gtcagctcaa tgataatgct ggcgcaggaa gcgtggtgca ttatgaacct
     4261 tctaatgttt tgttgatgac cggacgtgct gcagtgatga aacggttgat ggagattgtt
     4321 gaacgtgtgg ataaggtggg taatcgcagc gttgccacgg tcccgctcac ctacgcgtcc
     4381 gcaacagacg tagccagact tgttacggaa ctgactaaag aaacagataa gacagctata
     4441 cctgcttgga tgacggcgaa actggttgca gacgagagga caaactcagt gctcgtcagc
     4501 ggagagccaa tctcccaaca gcgtatcatc tccataatta agcaactgga tcgtcaggag
     4561 gatgttcagg gtaatactaa ggtgatttac ctgaaatatg cgaaggcgaa ggatttagtg
     4621 gaagtcctga caggtatcag cagcagtatt gaaaacgact ctaaaaagag tccgtcaacg
     4681 gaagccttgc gcaaaggagt gacgattaaa tcccacgaac aaaccaatgc cctgatcctg
     4741 acgggggccc ctgacgtcat ccgcgacctt gaaaatgtga tttcgcagtt ggatattcgt
     4801 cgtcctcagg tcctggtgga ggccatcatt gctgaaatac aggatgctga cgggctgaac
     4861 cttgggatcc agtgggtgaa taaacatgcc ggtgtggcgc agttcaccag taccggttta
     4921 cctattacca cgatggttca gactcgtcag aacgaaatct tagacagcga tcagagcaat
     4981 gccctgagca tgtttaacgg aattgcagcg gggttttatc agggaaactg ggcgatgctg
     5041 ttgacggcgc tctccacaag tagcaagaat gatatcttgg cgacccccag tattgtcacg
     5101 ctggacaata tggaggccac tttcaatgtt ggtcaggagg tcccggtact ttcgggctca
     5161 cagacaacct ctggggacaa tatttttaac acggtcgagc gcaaaacggt ggggatcaaa
     5221 ctcagggtaa aaccccagat caacgagggt gattccgtgt tactggagat agaacaggag
     5281 gtgtccggtg tggcggacac tgcagtagcc accactactg acttgggagc aaccttcaac
     5341 acccgaacag tgaccaatgc catgctggtc gggaatggcg aaacggtggt ggtcggagga
     5401 ttactggata agtcgatcag ggggagtgag agtaaagtgc cactgctggg ggatatcccg
     5461 gtactggggc atctttttcg cgcaaaaagc gaacagacag ctaagcgtaa tctgatgctg
     5521 ttcattcggc caactattat tcgtgagcgc gacggatttc gtcatgcttc ggccgaaaaa
     5581 taccagtcgt ttaatcagga acaggtgcag tcgcgtggca aagaaacaac ggcgctgacg
     5641 ctgaatgagg aacagctcag gctgtccccc gatcaagacg atacggcttt ccggaaggtg
     5701 aaagcggcga ttgctgcgtt ttatgcgcag gagatgtaat gagcagagta gtacagaatg
     5761 tcagtgaatc acgtcccctt ctgccttttt ccttttcccg tacgcagaga attttgttgc
     5821 tgcgtgaaca ggaggggaat cgagtgttct gcatggagga cacacctgcc agtgccctgt
     5881 tggaggtgcg cagggtggct gaaggtcctc tgaatgtcac aacagtgtcg gcggaagcgt
     5941 ttgaaaaaca gctggtgagc agctatcaac gtgattctga tgaagcgcga caaatgatgg
     6001 ctgagattgg taatgaaatg gatttttata ccgtcgcagg ggagttgcct gaccgtgagg
     6061 acttactgga tgcgaatgac gatgcgccaa ttatccgcct gattaacgca atgctgacag
     6121 aggcaattaa agagaaagcc tcagatattc atattgaaac ttacgaacgc catctgcagg
     6181 ttcgctttcg cattgatggc gttctgcggg agatcctccg tttacatagg aatctggctt
     6241 cgttactgat ttcacgcatt aaagtcatgg cccggttgga tattgccgaa aaacgcgttc
     6301 cccaagatgg ccgcatggta ctgcgtatcg gtggtcgggc ggtggatgtg cgtgtttcaa
     6361 cgttgccttc aaatcatggt gaacgcatcg tgctgcgttt actagacaaa aatagcgtta
     6421 gtctcgatct tgctgcactc ggcatgtcgc agcagaatca gcgacacatt gatgcactga
     6481 tccgccgtcc tcacggcatt attttggtga ccgggcctac agggtcgggt aaaagcacga
     6541 cgctttatgc cgcgttaagc ctgctgaatc cccgagaccg caacattatg acggtcgagg
     6601 atcctgttga atatgaactg gacggtatca gtcagaccca ggtcaacccg aaggtggaca
     6661 tgacctttgc ccggagtctg cgcgctattt tacgtcagga tccggacgtg gtgcttgtgg
     6721 gggagatccg tgacggtgaa acagcccaga ttgctgtgca ggcctcgctg acagggcatt
     6781 tggtgctgtc aacgttacat acgaatagtg cagcaggcgc gctgtcgcgt ctgcaggata
     6841 tgggcatccc tccttttctg ctttccacct cattactggc tgttctggcc cagcgactgg
     6901 ttcgcacgtt gtgtccgcgc tgtcgtcagc cctgtcaggt cagtactgag ttagcgatgg
     6961 acatggacat tccccctgaa accacgatct ggcagcctgc cgggtgtcag cactgcagtt
     7021 tcacgggcta tcacgggcgc accgggatcc acgagctgtt gctgattgac gatcgcattc
     7081 ggacggcaat ctatcagggg gagggggagc tgggtattac ccgcttggca gggagtcgct
     7141 atctgacgct gcgtggcgac gggcgacaaa aggtactagc cggtgagacc agttgggagg
     7201 aggtggttcg cgttactgaa agcagattgc aggaagagga atgatatggc gttgtttcat
     7261 tatcaggcat cagatattca cggtagaaaa cgcagtggca ttctggaggc ggattcggcc
     7321 cgacatgccc gtcagttgct gcgtgagcag gcgcttattc cagtcaggtt ggatgagaaa
     7381 caggtccatc acaagcactc actgcggagt atcctgaggt ttcgtccgcg cggggggagc
     7441 agcgccgaac tcgcgttact gacacggcag ttagccacac tagtggccgc atctctgccg
     7501 cttgaagagg cgctggatgc gttgttgagg cagagtgaaa aaccgcgtca gcggaactta
     7561 atcgctgccg tgcgcaccaa ggttctcgag ggacattcct tggccgcggc gatgggcatg
     7621 tttccaggta cgtttgagcg tctttattgt gcgatggtgg cggcggggga aacatccggt
     7681 cgtttggacg tggtgctcag tcgtctggct gactataccg agcagcgcca gatcatgcga
     7741 aaccgtctac tccaggcatt gctctatcct tgtgttctga cgttggtggc ggttggggtt
     7801 attgccattc tgcttactgc ggttgtgccg aaagtggttg agcagtttat tcatatgaaa
     7861 cagacccttc ccttatctac ccgcgtactg atgggggctg ctgaggtgag ccagacttgg
     7921 ggcccgtggc tgttactggc cgcagcgctg ggcgggatag cggggcgcat gatattacat
     7981 cagccctccc agcgtctggc ttttcatcat ctgctgttgc ggctgccggt cgtggggcgc
     8041 atatcgcgcg gtctgaatac cgcacgttat gcccgcacgc tgagcattct taatgccagt
     8101 gcggtaccgc tgctccaggc gatgcacatc agtggtgacg tactgagtaa tgactgggcc
     8161 cgtcatcagt tagctaccgc ggccgagttg gttagggaag gggtcagcct gcatcaggca
     8221 ctggagcaga cttcgctgtt tccgcctatg atgcggcaca tgattgccag cggtgaaaat
     8281 agtggcgaac tcgacagcat gttggaacgg gccgccgaca atcaggatcg tgagttcagc
     8341 acacagatgc aactggcgct gggattgttt gaaccgctgt tggtggtggg tatggccggg
     8401 gtcgttttgt ttattgttct ggcaattctg cagccgctcc tgcagctcaa caacatgatg
     8461 aatatgtgaa tccttcttta acagtcaacg gagaaaaaca tgcgaaaaca gcatcagcgt
     8521 ggttttacct tgctggaaat aatggtggta attgtgatct tgggagtttt ggccagtctg
     8581 gtggtcccga atctgatggg aaataaggat aaggccgatc ggcagaaagt gatgagcgac
     8641 ttggttgcgc tggaaagcac gctcgatatg taccggttag ataataaccg gtatccgaca
     8701 acggagcagg gactgcgggc actggtcagt aaacccaccg tgcaacctga accccgtaac
     8761 taccggcagg atggttatat ccgccgtttg ccccaggatc cttggggggg ggattatcag
     8821 ctgttaaatc ctggtcagta cagcgacatt gatatctttt ctcctggccc ggatggtgtg
     8881 ccaaacactg aagatgatat tggtaactgg acgctcggta acgcgcagcc atgagtcagc
     8941 gcggtttcac aatactggag atgatgctga tccttttgtt gatgggcatc actgcagggc
     9001 tggtactaat gtcatttcct gactctgctc agaatcatct gcaacaacag cgggagcgtc
     9061 tgcaggcgca gctggattat gccttggata gaagccagca ggacggcttg ctgatgggga
     9121 ttcaggttcg gtttgacgga tggaaattta aggttctgca gcgcggaaca gcggaaagcc
     9181 ccccgacatt agctgaggga ggggatattt ggcaggggta tgtctggcaa acttggcaac
     9241 cgcgacgtgc agccatgggg ggcaaacttc cggatgatgt gcgtcttgag ctgcagtatc
     9301 tcagaggttt gcagtggatg tctgagcatg atgacggggc tgaacccgat attttgctgc
     9361 ttcccggagg tgaggtaacg ccgttcagac ttcttttcag gcaggtcgga gaggaggccg
     9421 ttgtcgggct gcaggttgat gagaacggtt tgatgactct ttttgaaggt gaggtctcat
     9481 tgtgaagggg caagaaggaa tgacattact ggaagtcata gtagctctgg ttgtgtttgc
     9541 gctggcagga atggcgctga tgcaggcctc gacccagcag gctgcgggga tcggacggat
     9601 ggaagagaag gtgctggcag gctggttggc cgataaccag atggtgcaat tgcaactcga
     9661 gaaaacttgg ccggaaaatg gctggggcga gaagacgatc agcttcgctg gcaccgaatg
     9721 gtatttacgc tggggcgggc cggacagcga cgtgccgcag ccgcgctcct tagaggtaga
     9781 ggtacggcgc actaaagagg agacgtccgc cttggtttcc ttgcgtagca gtgtggttcg
     9841 tgaatgagtc agcaacgggt aaaaggtttt acgctactgg aaatgctgtt ggcactggct
     9901 gtttttgcag cgttgagcat cagcgcattt caggtgctgc agggtggaat acgggcgcac
     9961 gagctgtccc gggataaagt acagcgtctg gctgagctgc agagagggat cagccagatg
    10021 gagcgagatc ttacgcagat gctcccaagg cacagccggg ggaatgaagg cctgctgctc
    10081 gctgcacctc atctgctaaa aagtgatgac tgggggattt cttttacgcg aaacagctgg
    10141 ctgaatcccg ccgggatgct gccgcgcccg gagttacagt gggtaggtta tcggttgcga
    10201 cagcaaaagt tagaacgcct gagctacttt catgtcgatc acccttccgg tgtttctccg
    10261 gatgtcaggg tgatgctgga cggcgttcac gcatttcgtc tgcgtttttt tgttaacggt
    10321 gattggcagg cgcgctggga cagtaccggc atcctgccgc aggctgttga agtgacgctg
    10381 gttatggatg attttgctga actgccccgt ctgtttcttg tgagcaagga gaccgctgaa
    10441 tgaaattgcg ggaacagggc gtggcgcttc tggtggtatt gctgatcctg tcgttaatgg
    10501 taacgatagc ggcggtgatt gcggagcgca acggccggac atttttgcgt acagtagcgc
    10561 agttggatca gctgcaggca aagtgggatg gctatacggc agagacgata gcaaaacaaa
    10621 tcttacagag gagcaggcag gagtcgcccc ggaaaacgca tctggcacaa aattgggcgc
    10681 agtcagaacg acagtttgag accagagggg gagatgtgcg ggggcagatc gtggatgcgc
    10741 aggcctgttt taatcttaat gctattaatt atggagtggt ggatctgacc agtatacctt
    10801 atgccgccag aatttttcag cagctattaa tcaacttgca ggtagagctt ttacaggccc
    10861 ggcaagtgac ggcagcccta cgtgactgga tcgatcgtga tgataagccg gtaaggggcg
    10921 gtgctgaaga cgaggtgtat atggggatgg aacctcccta tctggcagcc aaccagccga
    10981 tgcaggacgt cagtgagtta cgccttatcc ggggaataga tgccaggctt taccgcaaac
    11041 ttttgccgta tgtttgtgta ctgcctacgt cagatctgtc tgtgaatgtg aatactctgc
    11101 tggattcaca ggcgccgctg ctggcggcgc tctttctgac gaaaccggac agtcttccgg
    11161 tgacggaatt actgcagcgg cgaccccgta ctggttggga gagcgtggcg gcgtttcttg
    11221 acgcgccacc gcttaaggac attgacacca gtgcggccat gccggtattg gccgtcagca
    11281 gcaactattt tctggttcgg ttacatgtgc gctccgggga acacctgttc agtcagcaga
    11341 ctttaatgca gtggagggag gagcgtttcc ggatcattca gcgacagtat ggattaacga
    11401 tgagggaggt tccctgaaaa aaaataacaa acatgtcgag gctggtcggc tttttctgcg
    11461 actgccggag gatgactcgg agcctttatt gtggaggctg attgcgccag aggagacagg
    11521 agccggcagt gtggcagtcg gagaaacgga tccgcagtta gtcggtttgc tgtcccgtta
    11581 tcccgcctgg gtgctggttc cgtcaggcag aattgccttc catcaggtca cgctgccaca
    11641 ccgcacgtgg cgtcagcgtt tgcaggtgct gccctttctt ctggaagagc agttggcgac
    11701 agacattgag cagctgcact tcgccattct tcatcagtcg ggtgacaggt gtgatgtggc
    11761 ggtggtcgaa aaagcggtta tgcatcgctg gttagcgtac tgtggacaac ttggggttcg
    11821 ggaggtgagg ttgctgcctg acgtactgat gctgccgttg gcaactgaag gatggagtgc
    11881 cgtaaagctt gccgggcagt ggctgtttcg ccgggatcgt tacgccggga tgacggtaga
    11941 gttatcgtgg ctgacacatt tcttgtctct tactgcgccg ccggtgattg agtcctactc
    12001 cgtgccgcca gtaccgtgcc cgggtccgaa gaccactgag tggcgaaatc agcccggacg
    12061 cgatctgctg cagttggtgg ccgaaggtga cgggtatgat ggcgcagatc tgcggcaggg
    12121 agaatttgcc cgccagggaa catggtatgc acgatttcgc ccatggaggt atgtcgcagg
    12181 ggctctgctg gcctgtgttt ttctggccgg gagcaacgcg gggctggcac attatcggct
    12241 ctggcagcag gctcaattct ggcggtatga gagcgtacgt gtctatcagc agctgtttcc
    12301 gagtgaaaag gcggtgaagg atccccgccg gcagatgctg agacatctgc aacagtcgac
    12361 gggtaacaat ataccggaac tcgggagcgt gatgcgccag cttcagcaac tgctgtcaga
    12421 aacctcaacg atacgcttac aggcactcgc ctgggacagc agcagccaga cactgaaaat
    12481 tgatttgcag gcggcttctt ttcaggcgct ggaacacttt cagcagatcg ccggagaaaa
    12541 atacctcatc cagcctggtg agatacgaca gcagccgcag ggggtagaaa gtcgtctgat
    12601 cctgagagtg aacaatgaac gagcttaaaa aacgtttgca gtatgttagc ccccgtgagc
    12661 gcaggctgct tatcggatgt gttgccttac tggttgttgt ttttgtctat tacgtcctct
    12721 ggcagccctg gctgatacga gaggataagt ggcggactgt agttacccgt gaaaaaaaca
    12781 cagtggagtg gatgaaacaa caggtaccca acataaagag acgggaggca agtatgcctg
    12841 aacaggggga gccgttaggt ttatctgcgg ccgtgacacg tacttcagcc gcatacggtg
    12901 tatcggtcac ccggttgcag cctcaagggg agcgtctggc cgtcacctta gccccggttg
    12961 aatttaatgc gctgatgcag tggcttacgc aactggagcg tcggcaccgt attcgtacca
    13021 tggtatttga tgtggccgcg cagggcaacc cacccggaca ggtaacggtt aatcggctgg
    13081 ttttgtccca agatgacgta aacaaggacg cttcgcttag tcgctgatgt cgtaatttac
    13141 agattaatgc ttgatgtaaa aattggactt ataaaggagg atgtgtgagt agtttgccgg
    13201 aatttgcggt tgattatccc ttgttgtggc tcatctgcac cggagggctg gggggcgtta
    13261 ccggcagttt tcttaacgtc gtgattcatc gtctgccgat aatgttggag aggcggtggc
    13321 ggcgtgaagc gctgacgcta ttggcacatt ctgtgcctga gtccgggccg gcctttaacc
    13381 tgatgatacc gcagtctcat tgtccctgct gttttcatcc gctcggcatc agggataaca
    13441 ttcccctgtt aagctaccta tttctgaagg ggaaatcacg ctgttgtggc gagcgtattc
    13501 ccgtttacta cccgctagtc gaaataacga actcagtgtt attcattctg gccgcgagca
    13561 ggtttccacc agggctgaca ttagcggctg cctggctttt tatttccatg ctgttggttc
    13621 tggcggttat tgactgccat acggcgctgc tgccggatgt gctgacgttg ccattgctct
    13681 ggcttggcct gttattcagt ttacagagag gcgttgtcac cttagaggag gcggtagtgg
    13741 gggcggtatc tgggtatctc tgtctgtggg gactgtactg gctttttcgt ttcgctaccg
    13801 ggagggaagg gttaggttac ggtgatttta agctagctgc ggcattggga gcatggatgg
    13861 gttggcaggc attaccctca atacttttgt ttgcctcagt cagtgggtta atcgtaacag
    13921 tactgttgcg tattgtaaca gctacggact ttacccgccc gctgcctttt gggccgtggc
    13981 tcgctttgag cggagtgtgc cattttttgt tgatgtagcg ttcggttacc ggggctagta
    14041 ataaaaacgg ctcagcggat aaaagaactg ttgtaaaccg gtatcaggtt aaacgatacc
    14101 tagtaaaaaa tgatgggtaa tattttgaaa aaactgaatt gtatcgcgtc attactagtc
    14161 ttagtgacta tctctggttg tcatcagtca ccctccatcc ataagcaggc cactgttcct
    14221 ccgagcgaac aactggaaca aatggcgtcg attgtctctg cgacacggta tctgaaaatg
    14281 agatgtaacc gtagtgattt accagatgaa cagtcgatcc taaatgtcgc taaccggatc
    14341 gctatcggga aagggtggca gtcattaact caggaggata tacgtaaaca cagcgatgat
    14401 atctatgtgc gtctgactag ggacagtacg cctgagtata taaaatgcag agaattcaat
    14461 cggcggctgg tcccatttat cggtgaatta ttggcaaggg gtcgtggata atatgaaccg
    14521 ccccggaaat cctggagact aaactccctg agaaagaggt aaacagcatg aataaaaatg
    14581 ggtcttccct tgtagtggtg gctgaaggca tgatgattgt agtggcacac tgaatttggc
    14641 cacctgaaca gaggtgatat gctcacctca gaacaacaca ggtgctccaa tgaaaaaaag
    14701 aaatttcagc gcagagttta aacgcgaatc cgctcaactg gttgttgacc agaactacac
    14761 ggtggcagat gccgccaaag ctatggatat cggcctttcc acaatgacaa gatgggtcaa
    14821 acaactgcgt gatgagcgtc agggcaaaac accaaaagcc tctccgataa caccagaaca
    14881 aatcgaaata cgtgagctga ggaaaaagct acaacgcatt gaaatggaga atgaaatatt
    14941 aaaaaaggct acaaccgcgc tcttgatgtc agactccctg aacagttctc gataatcggg
    15001 aaactcagag cgcattatcc tgtggtcaca ctctgccatg tgttcggggt tcatcgcagc
    15061 agctacagat actggaaaaa ccgtcctgaa aaaccagacg gcagacgggc tgtattacgc
    15121 agtcaggtac ttgagttgca taacatcagc catggttctg ccggggcaag aagcatcgcc
    15181 acaatggcaa ccctgagagg cttcagaatg gggcgctggc ttgccggcag gctcatgaaa
    15241 gaactgggac tggtcagttg ccagcagcct gcgcaccgtt ataaacgagg tggtcgtgaa
    15301 catgtcacta tcccgaatca ccttgggcgg cagttcgcag tgacagagcc aaatcaggta
    15361 tggtgcggcg acgtgacgta catctggacg gggaaacgtt gggcatacct tgccgttgtt
    15421 ctcgacctgt ttgcaaggaa accggtaggt tgggcaatgt cgttctctcc ggacagcaga
    15481 ctgaccatca aagcgctgaa aatggcctgg gaaatccgca gtaaaccagc cggggtaatg
    15541 ttccacagcg atcagggcag ccactataca agcaggcagt tccggcagtt actgtggcgt
    15601 taccagatca aacagagtct gagtcgacga ggaaattgct gggataacag cccgatggag
    15661 cgcttcttca ggagtctgaa aaacgagtgg ataccggtga cgggttacat gaacttcagc
    15721 gatgctgccc atgaaataac ggactatatc gttgggtatt acaacgcgct caggccgcac
    15781 gaatataacg gtgggttgcc accaaatgaa tcggaaaacc gatactggaa aaactctaaa
    15841 gcggtggcca gtttttgttg accactacat cccgtcagcc gatttacggg ccgtaagtaa
    15901 cgaagttgga tgcaaatgtc agatcgtgtg cgcctgttat ggctcggctg gtaagagagc
    15961 cttataggcg catttgaaaa aactccagct ataccggaag agatttatta ttaacaggag
    16021 aggtttgctt ttctgtgtag gtttagattt tgtgtttgca gggtaaacaa aactgattaa
    16081 ctcgtcattt cggtaactga aatagtttgc tgtattctgt ttacaatctc tttaataagg
    16141 catttgtcac gtggctattc atatgaaaat catacgttat cttacaaatc aatcatctga
    16201 gtgttataat ataacttagc tgtgatatgt gtaagaatgt ttaggcaata tattattatc
    16261 gctaatgttg ttttggttgg gagagattct cctt
//
LOCUS       DAZKFZ010000096        16244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000096 DAZKFZ010000000
VERSION     DAZKFZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(110..486)
                     /gene="rnpB"
                     /locus_tag="ACHOQW_002753"
     ncRNA           complement(110..486)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOQW_002753"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(519..1664)
                     /gene="garK"
                     /locus_tag="ACHOQW_002754"
     CDS             complement(519..1664)
                     /gene="garK"
                     /locus_tag="ACHOQW_002754"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP9573809.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1761..2651)
                     /gene="garR"
                     /locus_tag="ACHOQW_002755"
     CDS             complement(1761..2651)
                     /gene="garR"
                     /locus_tag="ACHOQW_002755"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9573810.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2681..3451)
                     /gene="garL"
                     /locus_tag="ACHOQW_002756"
     CDS             complement(2681..3451)
                     /gene="garL"
                     /locus_tag="ACHOQW_002756"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP9573811.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3467..4801)
                     /gene="garP"
                     /locus_tag="ACHOQW_002757"
     CDS             complement(3467..4801)
                     /gene="garP"
                     /locus_tag="ACHOQW_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP9573812.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5176..6747
                     /gene="garD"
                     /locus_tag="ACHOQW_002758"
     CDS             5176..6747
                     /gene="garD"
                     /locus_tag="ACHOQW_002758"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP9573813.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6896..7231
                     /gene="prlF"
                     /locus_tag="ACHOQW_002759"
     CDS             6896..7231
                     /gene="prlF"
                     /locus_tag="ACHOQW_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP9573814.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7231..7695
                     /gene="yhaV"
                     /locus_tag="ACHOQW_002760"
     CDS             7231..7695
                     /gene="yhaV"
                     /locus_tag="ACHOQW_002760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP9573815.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(7750..8559)
                     /gene="agaR"
                     /locus_tag="ACHOQW_002761"
     CDS             complement(7750..8559)
                     /gene="agaR"
                     /locus_tag="ACHOQW_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP9573816.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8808..10088
                     /gene="kbaZ"
                     /locus_tag="ACHOQW_002762"
     CDS             8808..10088
                     /gene="kbaZ"
                     /locus_tag="ACHOQW_002762"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP9573817.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10111..10584
                     /gene="agaV"
                     /locus_tag="ACHOQW_002763"
     CDS             10111..10584
                     /gene="agaV"
                     /locus_tag="ACHOQW_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP9573818.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            10595..11374
                     /gene="agaW"
                     /locus_tag="ACHOQW_002764"
     CDS             10595..11374
                     /gene="agaW"
                     /locus_tag="ACHOQW_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP9573819.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11364..12242
                     /gene="agaE"
                     /locus_tag="ACHOQW_002765"
     CDS             11364..12242
                     /gene="agaE"
                     /locus_tag="ACHOQW_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP9573820.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12260..12694
                     /gene="agaF"
                     /locus_tag="ACHOQW_002766"
     CDS             12260..12694
                     /gene="agaF"
                     /locus_tag="ACHOQW_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP9573821.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12691..13824
                     /gene="nagA"
                     /locus_tag="ACHOQW_002767"
     CDS             12691..13824
                     /gene="nagA"
                     /locus_tag="ACHOQW_002767"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9573822.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14175..15329
                     /locus_tag="ACHOQW_002768"
     CDS             14175..15329
                     /locus_tag="ACHOQW_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP9573823.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15342..16202
                     /gene="kbaY"
                     /locus_tag="ACHOQW_002769"
     CDS             15342..16202
                     /gene="kbaY"
                     /locus_tag="ACHOQW_002769"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP9573824.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         3888 a         4322 c         4173 g         3861 t
ORIGIN      
        1 tagacttaat aacttcttac aaggtctatt attaataccg tagatattta ttcatcttac
       61 cctgtgcacc aaaagcaaaa acccgccgaa gcgggttttt acgtaaatca ggtgaaactg
      121 accgataagc cgggttctgt cgtggacagt cattcatcta ggccagcaat cgctcactgg
      181 ctcaagcagc ctacccgggt tcagtacggg ccgtacctta tgaaccccta tttggccttg
      241 ctccgggtgg agtttaccgt gccacggact gttaccagcc gcgcggtgcg ctcttaccgc
      301 accctttcac ccttacctga tcccgcttgc gcgggccatc ggcggtttgc tctctgttgc
      361 actggtcgtg ggtttccccc ccaggcgtta cctggcaccc tgccctatgg agcccggact
      421 ttcctcccct ccgcccgtct cccccgaaga ggacgacgac gaagcggcga ctgtctggtc
      481 agcttcggcg cgcagtatag agggtttgcg cgcccttgtc accccgcgtt gcgcattcca
      541 atcgccagtg tcgcggcgat attacgtgaa gcacggcaga tattgtcata agccccgcgg
      601 aatgcttcgt ccaacgtacc tatgctggtc aatacgctga agaccgcatc aatgccatgc
      661 tgatgtacaa cgccaacatc atcggtcagg ctacccgcaa tgccaatcac cggtttatgg
      721 tacttcttcg ccacgtttgc gacaccaatc ggtaccttgc cgtgaatact ctggctgtca
      781 atacgccctt caccggtgat caccagcgta caatcgtgaa tatgttcctc cagattcagc
      841 gccgtagtga cgatttcaat accacttttc agttccgcac caagaaacgc cattagcgcc
      901 gcgcccatac cacccgcagc tcctgcgccg gggacatctt tcacatcaac atgcagcgct
      961 tttttaatga cctcggcata gtgagagagg ttattgtcca gctcaacaat catcgcttca
     1021 ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt tatcgcccac cagcggattg
     1081 gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc gcggatcgag gccggaaata
     1141 tcaatatcat tcagagtatt aagactaccg ccgccaaaac caatttcatt gccgttggcg
     1201 tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc cgccatcatt tgtagcgctg
     1261 ccgccaatgc cgataataat gtttgtcgcg ccgctctcca gcgcctgcag gattaactcg
     1321 cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt ccgcaggtac cagctccagc
     1381 ccactggccg ccgccatttc aataaacgcg gttttgccat cgccggagat cccccaactg
     1441 gcattcactt tctcgcccag cggccctgta acccaggcgt gacgttcgga accctgggtg
     1501 gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg caacaggaac agaaacgtac
     1561 tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca
     1621 gataaacttt ctttataaga gtctggggcg attacgattt tcatacctat gcctgttacc
     1681 acatgacgcc ggagggcgtt tctcttattc ggcctggatt ccaggcccgg attgcaatac
     1741 gccatccggg cacgacgtca ttaacgagta acttcgactt tcgccagttt ttcgtagtag
     1801 cacgccaggg cgctatgatc cgccgttcct aaaccatctg cacgcagtgc ctgcatcatc
     1861 tccataaccg cagctgtgag cggcagttgt gcgccgacgc cgtgagaagt atccagcgca
     1921 ttcgccagat ccttaatatg cagatcaata cggaagcccg gcttgaagtt gcggtccatc
     1981 accatcggcg ctttggcatc cagcacggta ctgcccgcca gtccaccgcg aattgcctga
     2041 taaaccaggt ccgggttaac gcccgcttta gttgccagcg ttaacgcttc tgacatcgcg
     2101 gcaatattca gcgccacaat gacctgattt gccagtttgg tgacgttacc tgcaccgatt
     2161 tccccggtat gcaccacgga acccgccatc gctttcatca aatcatagta tttgtcgaaa
     2221 atagccttgt cgccgcctac catcactgac agcgtaccgt cgatggcttt cggttcaccg
     2281 ccgctcaccg gagcatccag catatcaatg cctttcgctt tcagcgcttc gctgatttca
     2341 cggcttgcca gcggtgcgat agaactcata tcgatcaata ccgtacctgg cttcgcgcct
     2401 tcaataatgc cattctcacc cagcgccacc tctttcacat gaggggagtt tggcagcatg
     2461 gttatgatga cgtcgcactg ttcagcgatc gctttagccg tagaagctgt ttctgcacct
     2521 gcagcaatca cgtcagcaat agcttctggg ttacggtcag caaccaccag cgagtaacct
     2581 gctttcagaa ggtttttact cattggttta cccataatcc ccaggccaat aaaaccaact
     2641 ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg ttatttttta aaggtatcag
     2701 ccagtttctg agtggcagag cggaagacgc cgagatcgct gccgacagcc acaaacgtcg
     2761 cgccccattc cagataacga cgcgcatcgg cttcgaccgg cgcgaggata ccgctgggtt
     2821 tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc tttttgtaca tccgggtgtg
     2881 atgcattgcc gagatggcct aatgccgcgg ccagatcgct ggggccgacg aagatgccgt
     2941 ctacgccttc ggtagcggca atagcatcga cgttatctac gccctgctga ctttctatct
     3001 ggaccagaat agtgatgttc ttgttcgact gagcgaaata atccgccacg gtgccaaaca
     3061 tattggcgcg gtgagaaacg gagacgccgc gaatgccttc tggcggataa cgcgtcgatg
     3121 ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg aatcaggaag ttatagaaac
     3181 cgatatccag aagacgctta ataattaccg gctcgttggt cggcactcgc actactggcg
     3241 cgctggcgct gcctttcagg gccattaact gcggaataaa cgttgaaata tcgtttggcg
     3301 catgttcgcc atccagcacc agccagtcaa acccagccaa accaagaact tcagtgctaa
     3361 tcgggttaga gagtgctgac cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt
     3421 tgaatttatt cgggaaaaca tcgttattca tcgcttatac ctttgcttat ttctgcaatt
     3481 ccatacgttt aatgtcgcca actacgaaga ggtagcagac catcgccatt agcgctgaac
     3541 atcccacgaa aaccagtgct gcattgaagg agtgcagttc acttaccagg tagccaatca
     3601 ccagtggagt gacaatggag gcaacattgc caaagacgtt aaagacgccg ccgcagaggc
     3661 caacaatctc tttaggcgcg gtgtcagaaa tcaccggcca gcccagcgca ccaaatcctt
     3721 taccaaagaa agccagcgcc atcagcatga ccaccagcgt ggtgttgttg gtgtagttac
     3781 ataagatgat ggtggaagcc agcaacattc ccagcacaat cggtagctta cgtgccaggg
     3841 tcagggataa accgcgtttg atcagataat ccgagaagac acctcccagc acgccgcccg
     3901 caaaaccaca cagtgctgga atcgaggcga ccagacccac tttcagaatc gacatacctt
     3961 tttcctgcac cagataaatc gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa
     4021 aatattgtcc gaaaaatacg cccaacatca tgcggttaga gagcaattgc ttgatgtaat
     4081 gcagtttggg tccgcttgct gccgcactgc ccggcttttt gtggtccata tcgaccaccg
     4141 cgccattttc agagataaac ttcagctctt ccgcagacat acgtgggtga tctgtcgggt
     4201 tatgaatcaa tttgatccac agcgccgtca gcacaaaacc aatcaccccc ataacggtaa
     4261 agacgtgctc ccagccccag gcgaaagtca gccagccaag cagaggcgaa aagagcgcca
     4321 gcgagaaata ttgcgccgag ttaaagatgg cggaggcagt accacgttct ttcgtcggga
     4381 accaggcggc gacaatacgg gcgttcgccg ggaatgatgg cgcttccgag aagccgagca
     4441 taaagcgcat aaagaacatg gagatccctg cccaggccag cgggaacata tcaacaaagc
     4501 cttgcaggaa ggtgaatagc gaccagaaaa agaggctgta ggtgtaaact tttttcgagc
     4561 caaacttatc aagcagccag ccgccgggga tttgcatcag caagtaggcc cagccaaaag
     4621 cagaaaagat gtaacccatc gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac
     4681 cagcaataga cagcgttgca cgatcggcgt agttaacggc ggtaacaata aaaataatca
     4741 gtaatattaa atagcgggta tgcacgcctt tctttttttc gtcaacggtg tccagaatca
     4801 ttttatttac ctcgggtact tatgctgatt tttattatta tggggaagat gttatttatg
     4861 agtttcattt atgccgtaac gacaatgaac tcgggaatta gtataagcag cgcgagaata
     4921 ataatcattg tgcaaatgct aatttaatta atactattta aatattattt tgagcatatg
     4981 cacataaggt tgcgcgctaa agcacagatt tgcgctttac cttgccgggc ggcattgcaa
     5041 tccctgaaat gattgacatt gatcacattt ctgcgtttaa actcctgaca ttcttatttc
     5101 acccaatgaa gtcatttatt tttaaatgag accaggtcct cattttaata acctctggct
     5161 ggagaatatt gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat
     5221 ataaaagttc acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca
     5281 ggaacgcgtt ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc
     5341 gcattgctcg acattccggc taatggtgaa attattcgtt atggcgaagt gattggttac
     5401 gccgtgcgtg caatcccacg cggaagctgg atcgacgaat caatggttgt actgccgaaa
     5461 gcgccgccgt tacacacgct gccactggca accaaagtcc cggaaccctt accgccgctg
     5521 gaaggataca cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg
     5581 ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg tggactacgt agtaaaaatc
     5641 attgaacgcg atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat
     5701 ttgtacggtt gtggcgtggc gattaacgca ccggcggcag ttgtacctat ccgtaccatt
     5761 cacaatattt cgctgaatcc taactttggc ggcgaagtaa tggtgattgg cctgggttgt
     5821 gaaaagttgc agcctgagcg cctgctgact ggaacggatg atgtgcaagc tattccagta
     5881 gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg tcggttttca gtccatggtc
     5941 gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa
     6001 acctgtccgg cttcagaact ggttgtcggc atgcagtgcg gtggcagcga tgccttttct
     6061 ggcgtaacgg caaacccggc ggttgggtat gcgtctgatc tactggtgcg ctgcggcgca
     6121 acggtgatgt tctcagaagt cacggaagtg cgtgacgcga tccatctgct gacaccacgc
     6181 gcagtgaacg aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat
     6241 ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt
     6301 ctggcaaatg tagtggagaa agcgctcggc tccattgcta aatcgggtaa aagtgcaatt
     6361 gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg
     6421 ccagccagcg attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg
     6481 tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg tacccgtcat taaaatggcg
     6541 acccgcaccg agctggcgaa ccgctggttt gatttaatgg atattaacgc gggcactatc
     6601 gccaccggcg aagaaaccat tgaagaggtg ggctggaagt tgttccactt tattctcgac
     6661 gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg gactgcataa ccagctggcg
     6721 gtgtttaacc cagcaccggt gacctgattt cttttggaac gtccctcgca aaacatggcc
     6781 ttagtgccat gtttttattg tttaaagccc ccacgtccat taataatgca tttgcattac
     6841 cttagttcaa gcttataatt tgagcagaaa acaggctgta aaaggacagt gaatcatgcc
     6901 cgctaatgct cgctctcacg ctgtactgac cactgaatca aaggtcacga tacgcggaca
     6961 aacaactatc cccgcgccag tgcgtgaggc cttaaaactg aagccaggcc aggacagcat
     7021 tcattacgaa attctgcctg gtgggcaagt atttatgtgc cgactgggag atgagcagga
     7081 ggatcatact atgaatgcat ttttgcgttt tctggataca gatatccaga acaacccgca
     7141 aaaaactcgt ccattcaaca ttcaacaagg aaagaaactt gtcgctggca tggacgtcaa
     7201 cattgatgat gagattggcg acgacgaata atggattttc cacaaagggt taatggttgg
     7261 gcgctatatg ctcatccctg ttttcaggaa acctacgacg ctttagttgc cgaagtcgag
     7321 acattaaagg gaaaagatcc tgaaaattat cagagaaaag ctgccacaaa gttattggcg
     7381 gtggtccata aagtgattga ggagcatatc acggtcaatc catcatcacc ggcattccgt
     7441 catggcaagt cgttaggctc tgggaaaaat aaagactggt cacgggtaaa atttggtgct
     7501 ggtcgctatc gtctcttctt tcgttacagt gaaaaagaga aagtcatcat tctgggatgg
     7561 atgaacgatg aaaacacgct gcgcacctac ggtaaaaaaa cagatgccta taccgtattc
     7621 agcaaaatgt taaaaagagg acatcctcct gccgactggg aaaccctcac ccgagagaca
     7681 gaagaaaccc attgatggtg ttcacaatgc ccgccgaacg cattcgtgtt gaccagcggg
     7741 caccctttct cactccccga ccagaatcac ttcaacccca gcctttcgca gcccttccag
     7801 gctatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat
     7861 cttatgtaaa ctggaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc
     7921 aacttcgcac atccggcggt ttaaacgggc ttcatcttca ttatgcgtgc tgacgccgcg
     7981 ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga
     8041 ttgctcagcc tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat
     8101 cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat
     8161 cgcaattacg tcagtgtgct tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga
     8221 atcgaggatc acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg
     8281 tttcattgcg gtgttcagcg cgcttttatc ttccactgat ggctcgactg acggcgttgt
     8341 gctatcgcag atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa
     8401 cgccagatcg ttgcggatcg tcacggtaga tacgccatac aatgccgaca gatcgttaac
     8461 ctgcacactc ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc
     8521 tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact
     8581 ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt
     8641 gcccattgac gcaagctact ctcgtttcag tgactttcat tatgtttctt ttgtgaatca
     8701 gatcagaaaa ctattatctt tcgttttatt tttatctcac catgacgcag tatcaactga
     8761 aacaaaacga aagattaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga
     8821 cagaaatggt gagacagcac aaagcgggca aaacaaatgg aatttatgcc gtttgttccg
     8881 cacatccgct ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac
     8941 tgattgaagc aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg
     9001 ccgattttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgt
     9061 tgattctggg tggtgaccat ctggggccaa accgctggca aaacctgccg gccgctcagg
     9121 caatggccaa tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc
     9181 accttgattg cagcatgtcc tgtcaggacg atccgattcc cttaactgat gacatcgtgg
     9241 ctgaacgcgc cgcccgtctg gcgaaagtgg cggaagaaac ctgtcttgaa cactttggcg
     9301 aagccgatct ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa
     9361 ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc
     9421 gtcacgcctt tgaaaagcaa ggtttgaatg ccatctggcc acgcatcatt gccctggtgg
     9481 ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga
     9541 ccgccttaag ccagatggtc gaaagctacg aaacgctgat tttcgaagcg cactctaccg
     9601 attatcaaac gccgcaatcg ctgcgccagc tggtgattga ccactttgcc attctgaaag
     9661 ttggcccggc gctgaccttc gccctgcgtg aagctctgtt ctctctggca gcgattgaag
     9721 aagaactggt gccagcaaaa gcctgttctg gtctgcgtca ggtgctggaa aacgtgatgc
     9781 tcaaccgccc ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg
     9841 cgcgtggtta tagctactcg gatcgcgtgc gctattactg gccggacagc cagattgatg
     9901 acgctttcgc tcatctggta cgtaatctgg cggattcacc aattccgctg ccgctgatca
     9961 gccagtatct gccgctgcag tacgtgaaag ttcgctccgg cgagctgcag ccaacgccac
    10021 gggaactcat tatcaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag
    10081 gccaataagc aaaacaaaga ggaacacgct atgccaaata ttgttttaag ccggattgat
    10141 gaacgcttga ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg
    10201 gtgctggtcg ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg
    10261 gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagttat cgacaacatt
    10321 catcgcgccg ccgatcgaca gaaaatcctg ctggtttgta aaacacccgc cgatttcctg
    10381 acgctggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc
    10441 aatggcaagc aacaaatcgc caaaacggtt tctgtggatg cgggcgatat cgcagcattt
    10501 aacgacctga aagccgctgg ggtggaatgc ttcgttcagg gcatcccgac agagcctgct
    10561 gtggacctct ttaaattact ttgagggatt catcatggaa atcagtctgt tgcaggcatt
    10621 cgcgttgggc attatcgcct ttatcgctgg cctggatatg tttaacggct taacccatat
    10681 gcaccgccca gtggtcctcg gcccgttagt cgggctggta cttggcgatc tgcataccgg
    10741 tattttaacc ggcggtacgc tggaactggt gtggatgggg ctggccccgc tggcgggcgc
    10801 acagccgcct aatgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg
    10861 cgtgaaaccc gatgtcgcag tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg
    10921 gattaccttc ctgttctcgg tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga
    10981 gaacgccgat actcgcggca ttgaacgcgt aaactatctg gcactgctgg cactcggcac
    11041 tttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat
    11101 cattgatgtc ctgccgcaac gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc
    11161 agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca tcccctactt
    11221 catcctgggc tttgttgccg cagcctggct caagttaccg gtgctggcta ttgctgccgc
    11281 cgcgctggcg atggcgctga tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc
    11341 ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagtg
    11401 aaaacgaaga aacattgctg actggcgtca atgaaaacgt gtatgaagat cagagcattg
    11461 gcgcggagct gacgaaaaaa gatatcaatc gcgtcgcctg gcgttccatg ctgttacaag
    11521 cgtcttttaa ctacgaacgt atgcaggctt ccggctggtt gtacggtctg ctgcctgctc
    11581 tgaaaaagat ccacactaat aaacgcgacc tggcgcgcgc catgaagggg catatgggtt
    11641 tcttcaatac ccatccgttt ctggtgacat ttgttatcgg cattatcctt gcgatggagc
    11701 gttctaagca ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgctcg
    11761 gcgggattgg cgatgcaatg ttctggctaa cgctactgcc gatttgtggc gggattggag
    11821 ccagtctggc attgcaaggc tccattcttg gcgcagtcgt ctttattgtg ctgttcaacg
    11881 tggtccacct ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg
    11941 cgattccact cattaaagct aataccaaaa aagtcggcca tgctgcgtct atcgttggga
    12001 tgacggtaat cggtgcgctg gtggcaacct atgttcgttt aagcaccacg ctggaaatca
    12061 cagcgggcga cgcagtggtt aagttacagg ctgatgttat cgacaaactg atgcctgcct
    12121 tcttaccgtt ggtctacacc ctgaccatgt tttggttggt acgccgcggc tggagtccgc
    12181 tgcgtctgat tgcagttacc gtggttctcg gcatcgttgg taaattctgc catttccttt
    12241 aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg ggtttgccag
    12301 cggcatggaa aaagcaatga agcaaattct cggtgagcaa tcgcagttta tcgccatcga
    12361 ttttccggaa acctcaagca ccgcgctgct tacatcgcag cttgaagagg ccatcgccca
    12421 actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg
    12481 cgtagcttca acgctggcga tgcaaaaacc gggctgtgaa gtgatcaccg gcaccaattt
    12541 gcaactgcta ctggagatgg tgctggagcg cgaagggtta agcggtgaag aattccgtgt
    12601 acaggcgctg gagtgtggac atcgtgggct gaccagtctg gtggacgagt taggccgctg
    12661 ccatgaagaa tgtccggtcg aggaagggat atgacacacg ttctgcgcgc cagaaggctg
    12721 ctgactgaag agggatggct cgatgaccat cagttgcgta ttgctgacgg tgtcatcgca
    12781 gcaatcgaac cgattccagt gagcgtgact gaacgcgatg cggaactgct ctgccccgct
    12841 tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat
    12901 gtactcgaca agctggcaat gcacaaggca cgcgaaggtg tcggcagttg gttgccgact
    12961 accgtaaccg cgccgcttag taccattcat gcggcgctga aacgtattgc tcaacgttgc
    13021 caacgcggcg gacctggtgc gcaagtgctg gggagttatc tcgaaggacc gtacttcacg
    13081 ccgcagaata aaggcgcgca tccgccggag ttgtttcgcg agcttgaaat tgccgagctg
    13141 gatcaattga ttgccgtttc tcagcacacc ttacgcgtgg tagcgctggc accggaaaaa
    13201 gagggggcat tgcaggccat ccgccatctt aaacagcaaa atgtacgagt gatgctgggg
    13261 catagcgcgg cgacctggca acaaactcgc gccgcgtttg atgctggtgc cgacggcctg
    13321 gtgcattgct ataacgggat gacaggttta catcaccgcg aaccgggaat ggttggcgcg
    13381 ggattaacgg acaagcgcgc ctggctggaa ctgatagccg atggtcatca tgtgcatccg
    13441 gcggcaatgt cgctgtgttg ttgctgtgcg aaagagagaa tcgtactgat caccgacgcg
    13501 atgcaggcag ccgggatgcc ggatggtcgc tatacgttat gtggcgaaga agtgcagatg
    13561 cacggtggcg ttgtccgtac cgcgtccggt gggctggcgg gcagtacgct gtctgttgat
    13621 gcggcagtgc gcaacatggt cgagttgacg ggcgtaacgc ctgcggaagc cattcatatg
    13681 gcgtcgctgc atccggcgcg aatgctgggt gttgatggtg ttctgggatc gcttaaaccg
    13741 ggcaaacgcg ccagcgtcgt tgcgctggat agcgggctgc atgtgcaaca aatctggatt
    13801 cagggtcaat tagcttcgtt ttgatggttt gctccattat tgggccttca cttcccccgt
    13861 aaggcctttc tttttctttc gttttgatct gtgcagcggt gtcggatgcg acgctaacgc
    13921 gtcttatccg acctacagtt ggtgaccgca aggccggata aagcgtttgc gccgcatcca
    13981 gcaatccctt ttgcttcctt tatcttttct ttcaacgatc acggatttcg ttttatttcc
    14041 tttttctcca ttgaactttc ggtttctttt ctatagattt taatcaacga aagacatcac
    14101 caagtgaaat gaaacgaaag gcaagtgaaa gcgacaacgc ccgacgtcaa gttcatcaga
    14161 ctaaggattg agttatgcca gaaaattaca cccctgctgc cgccgcaacc ggtacatgga
    14221 ctgaagaaga gatccgccat cagcctcgcg catggatccg ttcgctcacc aacatcgacg
    14281 cgctacattc cgcgcttaat aacttccttg aaccgttact gcgcaaagag aatctgcgga
    14341 tcatcctgac cggagccgga acctcggcat ttatcggtga catcatcgcg ccgtggctcg
    14401 ccagccatac cggtaaaaac ttcagcgccg taccgaccac cgatctggtc actaatccga
    14461 tggactacct gaacccagcc catccgctgc tgttgatctc cttcggtcga tccggcaaca
    14521 gcccggaaag cgtcgcagcc gtggaactgg caaatcaatt tgtaccggaa tgctatcacc
    14581 tgccgatcac ctgcaacgaa gcgggcgctc tttaccaaaa cgcgatcaac agcgataacg
    14641 cgtttgccgt gctgatgccc gcagaaacgc acgatcgcgg ctttgcgatg accagcagca
    14701 ttaccaccat gatggccagc tgcctcgcgg ttttcgcacc tgagacgatc aacagccaaa
    14761 ccttccgcga cgtggcggat cgttgccagg cgatcctgac ctcactgggc gatttcagcg
    14821 aaggtgtgtt tggttacgca ccgtggaaac ggatcgttta tctcggcagc ggtggcttac
    14881 agggcgcagc acgcgagtcg gcgctgaaag tgctggaact gacggcgggt aaactggcgg
    14941 ccttttatga ttctccaacc ggattccgtc atggaccaaa atcgctggtc gataacgaaa
    15001 cactggtggt ggtatttgtc tccagccacc cttacacccg tcagtatgat cttgatctgc
    15061 tggctgaact tcaccgtgac aaccaggcaa tgcgtgtaat cgccatcgcc gcggaaagca
    15121 gcgacatcgt cgctgccggt ccacatatca tcctgccacc gtcacgtcac tttatcgacg
    15181 ttgagcaggc attttgcttc ctgatgtacg cccagacgtt tgcactgatg cagtcgctgc
    15241 acatgggcaa tacgccggat accccatcag ccagtggcac cgttaaccgc gtggtgcaag
    15301 gcgtaatcat tcatccgtgg caggcataag aggatcgcat tatgagcatt atctccacca
    15361 aatatctgtt acaggacgcc caggccaatg gctacgcggt gcctgctttt aacattcata
    15421 acgccgagac gatccaggcg atcctcgaag tgtgcagtga aatgcgatcg ccggtgatcc
    15481 tcgccggaac gccggggacc tttaaacata tcgcgctgga agagatctac gccctgtgca
    15541 gcgcctattc cacaacctac aacatgccac tggcgctgca tctcgaccac cacgaatcgc
    15601 tggatgatat tcgccgtaaa gtccacgcag gtgtgcgcag tgcgatgatc gacggcagcc
    15661 acttcccgtt tgccgagaac gtaaagctgg tgaaatcggt tgtcgacttc tgccactccc
    15721 aggattgcag cgtggaagca gaactgggcc gcctgggcgg tgttgaagat gacatgagcg
    15781 ttgacgccga aagtgcattc ctgaccgatc cacaagaagc taaacgcttt gtcgaactga
    15841 ctggcgtcga cagcttggcg gtagcgattg gtacggcgca cggcttatac agcaaaacgc
    15901 cgaagattga tttccagcgg ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc
    15961 tacatggtgc cagcgatgtt ccggatgaat ttgtccgtcg cactattgaa cttggcgtca
    16021 caaaagtgaa cgttgccaca gaattaaaaa tagccttcgc cggcgcggtt aaagcctggt
    16081 ttgcggaaaa tccgcagggt aatgatcctc gttattatat gcgcgtcgga atggatgcga
    16141 tgaaagaagt tgtcagaaat aaaattaatg tctgtggttc agcgaatcga atttcagcat
    16201 aatcattagt tttttatgat ttatcccaat gtacttcccg atta
//
LOCUS       DAZKFZ010000097        16229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000097 DAZKFZ010000000
VERSION     DAZKFZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(272..697)
                     /gene="arsC"
                     /locus_tag="ACHOQW_002770"
     CDS             complement(272..697)
                     /gene="arsC"
                     /locus_tag="ACHOQW_002770"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP9573825.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(710..1999)
                     /gene="arsB"
                     /locus_tag="ACHOQW_002771"
     CDS             complement(710..1999)
                     /gene="arsB"
                     /locus_tag="ACHOQW_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP9573826.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2053..2406)
                     /gene="arsR"
                     /locus_tag="ACHOQW_002772"
     CDS             complement(2053..2406)
                     /gene="arsR"
                     /locus_tag="ACHOQW_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP9573827.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3150..3233
                     /gene="dinQ"
                     /locus_tag="ACHOQW_002773"
     CDS             3150..3233
                     /gene="dinQ"
                     /locus_tag="ACHOQW_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP9573828.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3287..4639)
                     /gene="gorA"
                     /locus_tag="ACHOQW_002774"
     CDS             complement(3287..4639)
                     /gene="gorA"
                     /locus_tag="ACHOQW_002774"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9573829.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4711..5553)
                     /gene="rlmJ"
                     /locus_tag="ACHOQW_002775"
     CDS             complement(4711..5553)
                     /gene="rlmJ"
                     /locus_tag="ACHOQW_002775"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP9573830.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5756..7798
                     /gene="prlC"
                     /locus_tag="ACHOQW_002776"
     CDS             5756..7798
                     /gene="prlC"
                     /locus_tag="ACHOQW_002776"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP9573831.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7806..8558
                     /gene="rsmJ"
                     /locus_tag="ACHOQW_002777"
     CDS             7806..8558
                     /gene="rsmJ"
                     /locus_tag="ACHOQW_002777"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP9573832.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8607..10076)
                     /gene="dtpB"
                     /locus_tag="ACHOQW_002778"
     CDS             complement(8607..10076)
                     /gene="dtpB"
                     /locus_tag="ACHOQW_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP9573833.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGTFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10242..10295
                     /locus_tag="ACHOQW_002779"
     CDS             10242..10295
                     /locus_tag="ACHOQW_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573834.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10273..10326
                     /locus_tag="ACHOQW_002780"
     CDS             10273..10326
                     /locus_tag="ACHOQW_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573835.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10393..10827)
                     /gene="uspA"
                     /locus_tag="ACHOQW_002781"
     CDS             complement(10393..10827)
                     /gene="uspA"
                     /locus_tag="ACHOQW_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP9573836.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11218..11553
                     /gene="uspB"
                     /locus_tag="ACHOQW_002782"
     CDS             11218..11553
                     /gene="uspB"
                     /locus_tag="ACHOQW_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP9573837.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11624..13123)
                     /gene="pitA"
                     /locus_tag="ACHOQW_002783"
     CDS             complement(11624..13123)
                     /gene="pitA"
                     /locus_tag="ACHOQW_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP9573838.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13355..14557
                     /gene="yhiN"
                     /locus_tag="ACHOQW_002784"
     CDS             13355..14557
                     /gene="yhiN"
                     /locus_tag="ACHOQW_002784"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9573839.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(14875..15927)
                     /gene="yhiM"
                     /locus_tag="ACHOQW_002785"
     CDS             complement(14875..15927)
                     /gene="yhiM"
                     /locus_tag="ACHOQW_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP9573840.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4180 a         4098 c         4212 g         3739 t
ORIGIN      
        1 taatccagta cgtttttaaa ctgctgcact gtatcgctga tcactaagca gctcactgat
       61 acatcatgct ggacgcgata attggaatct cacgcaggga gtaatgtgta aatagcaaaa
      121 tcgcctgatg cgctacgcct gtcaggccta caaataacat acaatttatt gaattttcat
      181 gactttgtag atcggataag acgcgttagc gtcgcatccg acataaataa agcgcacttt
      241 tctaacaacc tgtggggggg atatagccgc tttatttcag gcgcttaccc gcttcatcaa
      301 ccactttctc gccatcttcc ttggtgaatg cgcctttttg cgcatctggc agaatttcca
      361 gcaccacttc tgaagggcgg cacaggcgag ttcccagcgt cgtcaccaca atcgggcgat
      421 taatcagaat cgggtgctga agcataaagt cgattaaccg atcgtcagta aatttatctt
      481 ccgcaaggcc cagctcctca tacggttcga cgtttttacg cagcagcgcg cgtacggaaa
      541 tccccatatc ggcaatgagt ttgaccagtt catcgcgcgt tggcggagtt tccagataat
      601 ggataatggt cggttctgtg ccgctgttgc ggatcatctc cagcgtatta cgcgacgtgc
      661 cgcaggccgg gttgtgataa atggtaatgt tgctcatatc agtatctcat tacaaagtga
      721 aagagagacg tagcgccagc gcagccagcg tcacaaacag cacaggcagg gtcatgataa
      781 tccctgtacg gaagtaatat ccccagctga tagtcatatt cttctgcgaa agtacgtgca
      841 gccagagcag cgttgccagg ctaccaatcg gggtgatttt cggccccaaa tcgcagccaa
      901 tcacgttggc ataaaccatc gcttctttga taacgccaga tgccgtgctg ccatcaatgg
      961 acaacgcgcc aaccagtacc gtcggcatat tgttcataat agaagagagg aaggcggtga
     1021 ggaatccggt gccgagcgtc gcggcccaca ggccgttatc cgccagcacg ttgagtacgc
     1081 cagaaagata ttccgttaat ccggcattgc gcaggccata aaccaccaga tacatgccga
     1141 gcgagaagat gacaatctgc cagggcgcac cgcgcagcac tttacccgta ttaatcgcat
     1201 gaccgcgttt agcgacgaca aataatatca acgcgcccac tgccgcaatg gcgctgaccg
     1261 gaatgccgag cggttccagg acgaaaaatc ccaccagcag aagcagtaaa acaacccagc
     1321 cagttttgaa cgtagcagga tctttaatcg cttctgcggg agatttcagc agcgccatgt
     1381 cgtagttctg cggaatatct ttgcggaaat agagatgcaa catcaccagc gtggcaacaa
     1441 tcgcggcgat atccaccggc accatcaccg aggcatattc gcgaaagccg aggccaaaga
     1501 aatctgcgga aacgatattc accaggttgg agacaataag cggcaggctg gcggtatcgg
     1561 caatgaatcc ggccgccatc acaaacgcca gcgtagtgcc tttactgaac cctaaagcca
     1621 gcagcatggc gatgacaatc ggtgtcaaaa taagcgccgc gccatcattg gcaaacaggg
     1681 cggcaacggc agcaccgagc aggacaatcc aggtaaacag caagcgaccg cgtccattgc
     1741 cccagcgtga aacgtgcagt gccgcccatt caaaaaagcc ggactcatcc agcagcaggc
     1801 tgatgataat aacggcaata aacgcagccg tcgcgttcca gacgatattc cacaccaccg
     1861 gaatatcgcc cggatgtact acgcccgtaa ctaacgccag tactgcgccg agcgttgcac
     1921 tccagccgat gcctaaacct ttcggctgcc agataaccaa tacaatggtc aggacaaaga
     1981 tagcgcctgc cagtaacata atgcctcccg gaaaaacaca tgtgcaaatc catatgtgtt
     2041 tagataaatt ttttaactac aagtgttctt actgtctacg gaacaatttt gtctggccag
     2101 gttgcggaca atcacctgaa ccttttcctg ttcgcatcgc caggcctgct caataatttt
     2161 cgcggcccat gatggaatat gcggtgataa gcggtaatga acccacttac cctgcttgcg
     2221 atccagcaac aaaccgcttt cacgaagaag cgccagatgg cgggagatct tgggctgcga
     2281 ctggtcgaga gcagtgcaga gatcacagac gcataactct cccagctcgc tgagcagtaa
     2341 aacgatgccc agacgggttt catcggcaag aattttgaac agttggatgg gtaacagaaa
     2401 tgacatattg cgctcctgat tgttgctggt agtgtctctc ttcgaagcgg ataagtcaaa
     2461 aacatatatg gtttaacgaa tgtgtatgtg cagaggaagg taataggtgt gaattaagcg
     2521 ttggctattc atttgaaagg aggtctgaat caggacgtaa aaaaaccgac tttgcgtcgg
     2581 ttttttactt tccagcccag agttggtggc tctggctgga gtgagaatgc ccacatctaa
     2641 cggttcaata ctattagcca gtggctaaca ttgcaagcgt tagcgatttc ttcagctgga
     2701 gaagggacag tttgctatac atgttggtga aagaggattt atatactttc atcgggtcgc
     2761 tgccgtacca ggctttgtcc gaatccggat gtaaaaaaac cgacttcgcg tcggtttttt
     2821 tactcttcag ccctgagttg gtggctctgg ctggagtgag aatgccatca tctaacgcat
     2881 caatactaat accatactga aactattgca aggacatgct ggttttataa cctgaatata
     2941 ctgtatgatt atccagttag ctctgaggca ttttcactct ggcaatgcgc ataaacgctt
     3001 tcaaagtcct gttcagtggt acgggtggtg ccgttaactg atgctctggc cggagtgaga
     3061 ataccattat ctaacaatag ggcatgcgcc gtgacaggcg atggatgagt aagcggatgc
     3121 attctcactc catcgtatgg agaacacggg tgattgataa agcaatcatc gttcttgggg
     3181 cgttaattgc gctgctggaa ctgatccgct ttctgcttca gcttctgaac tgatagcgga
     3241 aacgtaatta agggcaaaga gcacaccact ctttgccctt taacatttaa cgcatggtca
     3301 cgaactcttc tgccgccgtt gggtgaatgg cgacggtatt atcgaagtct tttttggttg
     3361 cgcccatctt cagtgccacc gcgaagccct gcaacatttc gtccatacca aaaccaatgc
     3421 cgtgaatacc gacaatcttc tcttctggcc caacgcacac cagcttcatg cggcacggct
     3481 ggcggtgagt ggtgacggcg gtatacatcg cggtgaaaga ggatttatac actttcacct
     3541 gatcgtcgcc gtactgctcg cgcgcctgcg gttccgttaa accaacagta ccaatcggcg
     3601 gatggctgaa gaccacggtc ggaatgttgc tgtaatccag atgctcatcc ggcttgttat
     3661 taaacaggcg ttcagagaga cggcgacccg ctgcaactgc caccggtgtc agttctactg
     3721 cacccgtgtt atcgcccacc gcgtaaatac cttcaacgtt ggtgttttga tatttatcga
     3781 cgacgatata gcctttttcg ttggttttaa cgccagcggc ttcgaggttg atgttgtcat
     3841 tggcaggctc gcggccaatc gcccaaatca ggcaatccac cgtttcactg cgaccatctt
     3901 ccagctccag cgtcaggcta ccatcggcat ttttcactac cgctttcggg atggcgttgg
     3961 tgtgcaactg cgggccttcg gcgttcatca cttcgaccag cgtttcagag atcatcgggt
     4021 cgaagctgcg cagcggcgca tgtttacgca caaacagatg cgttttcgcg ccgaggccgt
     4081 taatcacgcc agccagctca acggcgatgt aacccgcgcc aacaaccgcc acacgctctg
     4141 gcaaagcagg aagggcaaag aaaccatcag aatcaatacc gtattccacg cccggaatat
     4201 ccgggtggct cggacgaccg ccggtggcga tcagaatatg atcggctgtg atggtttcac
     4261 cgttaacttc cagcgttttg gcatcaacga agcgggcaaa gcctttgatt acatcaacgt
     4321 tatttttacc gagcacgttt tcataggaag tatgaatacg gtcgatatag gcggtacggc
     4381 tggcgatcaa cgtttcccag ttgaatttat tgatagtggt atcaaaacca taatccgggc
     4441 cgtacatatg gatcgcttca cggatttgcg ccgcgtgcca catcactttc ttcggtacgc
     4501 agccgacgtt cacgcaggtg ccgcccagct ctttggcttc aatcagcgcg catttctgac
     4561 cgtacatagc cgcgcggttg atggaggcga taccgccgct gccgccgccg atggcgatgt
     4621 aatcatagtg tttagtcatg acagagtgtc cttatcgttg attaccgcga ttgtagcgcg
     4681 agacgtaata ggtgccagca atggctgcaa ttactctggc acgatccagc ttacggtggc
     4741 gtgcccggtg ccagctggaa ccagtttgct gtgcagccac ggcagcacgt tattcatctg
     4801 ctgttccagt ttccacggcg ggttaatcac aatcatgccg gaagcggtca tgccacggcg
     4861 atcgctgtct ggcagtaccg ccagttcaat ttgcagaatt ttgcgaatgc cggtcgcttc
     4921 caggtcgtgg atcatgcgct taatttgctg gcgcagcacc accggatacc acaatgcgta
     4981 agtgccagtg gcgaaacgtt tgtaaccttc tgctatcccg ctgactaccg cctgatagtc
     5041 ggttttcatt tcatacggcg gatcgataag gattaaacca cggcgagaaa ccggcggcag
     5101 tttggcctta agctgctgga aaccgtcgga tttttcgacg cgcgcacggc tgtctttctg
     5161 aaattcagaa cgcagcaacg ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc
     5221 ctgttcacgc agtaactgac gggcaatcaa cggtgaaccg gggtagtaac gcaactgacc
     5281 gctacggttg aagtgtttta ccacattgat gtacggctcc agctccgcag gcaaatcgtc
     5341 ctgctgccag atacgggcga taccttcgag atattcgccg gtacgctcgg catgttcgct
     5401 gcctaactga taacgcccgg cccctgcgtg ggtgtcgaga tagagaaacg gtttatcttt
     5461 ctctttcagc gactcgatga tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt
     5521 gccagcgtga aagctgtggc gataactgag catgggtaaa ggtgttccgg taagtaaaaa
     5581 tcggcccgta ttcggggcgc acaaaagcct atcaggacag tataccgaaa agaggccgcc
     5641 gccgcgaaag cgtaacgttt ctcattgaaa ttcactacac ttaaccccat gctacaaaca
     5701 ttatgtaaag cgcctgttga gcgcttcctt aacctcttta accaggactg cgctaatgac
     5761 gaatccgtta ctgactccct ttgaattgcc tccgttttct aaaattctcc cggaacatgt
     5821 cgttccagcc gtgactaagg cattgaatga ctgccgcgaa aatgtggagc gcgtagtagc
     5881 gcaaggagca ccgtacacct gggaaaatct ctgccagccg ttggcggaag tggacgatgt
     5941 gttggggcgt attttctccc cggtcagcca cctgaactcg gtgaaaaata gcccggaatt
     6001 gcgcgaagcg tacgaacaaa ctctgccgct gctgtcggaa tacagcacct gggtagggca
     6061 acatgaaggg ctgtataaag cgtaccgcga cctgcgcgat ggcgatcatt acgccacgct
     6121 gaacacggct cagaaaaaag cggttgataa cgcactgcgc gattttgaac tctcaggcat
     6181 cggtctgccg aaagagaaac aacagcgtta cggcgaaatt gccactcgtc tttctgaact
     6241 gggcaaccag tacagcaaca acgtccttga tgcgacgatg ggctggacta aactcgttac
     6301 cgacgaagcg gaactggcgg ggatgccaga aagcgcgctg gctgcggcaa aagcccaggc
     6361 cgaagcgaaa gagctggaag gctacctgtt gacgctggat atcccaagct acttgccggt
     6421 aatgacctac tgcgacaacc aggtcttgcg tgaagagatg tatcgtgctt acagcacccg
     6481 cgcttcagat caaggtccaa acgccggtaa atgggataac agtaaggtga tggaagagat
     6541 cctcgcgctg cgtcacgaac tggcgcaact gctgggcttt gaaaactacg cgtttaaatc
     6601 actggctacc aaaatggcgg aaaacccgca gcaggtgctt gatttcttaa ccgatctggc
     6661 aaaacgcgcg cgtccgcagg gcgaaaaaga gctggcgcaa ctgcgtgcct tcgccaaagc
     6721 cgaatttggc gtggatgagt tgcaaccgtg ggatatcgca tactacagcg aaaaacaaaa
     6781 acagcacctc tacagcatca gcgacgaaca actgcgtccg tacttcccgg aaaacaaagc
     6841 ggttaacggc ttgtttgaag tggtaaaacg tatttacggc atcaccgcta aagagcgtaa
     6901 agatgttgat gtctggcacc cggatgtacg tttcttcgaa ctgtatgacg agaacaacga
     6961 actgcgcggc agtttctacc tcgacctgta tgcccgtgaa aacaagcgcg gcggggcgtg
     7021 gatggatgac tgcgtaggcc agatgcgtaa agccgatggt tcgctgcaaa aaccggtcgc
     7081 gtatctgacc tgtaacttca accgcccggt gaatggtaaa ccggcgctgt tcactcacga
     7141 cgaagtgatc actctgttcc acgagttcgg tcacggcctg caccatatgc tgacccgcat
     7201 cgaaaccgcc ggtgtttccg gtatcagcgg tgtgccgtgg gatgcggtcg aactgccgag
     7261 tcagtttatg gaaaactggt gctgggagcc ggaggcgctg gcgtttatct ctggtcacta
     7321 cgaaaccggc gaaccgctgc cgaaagagtt gctggataaa atgctggcgg cgaagaacta
     7381 ccaggcggcg ctgtttattc tgcgccagct ggagttcggc ctgttcgatt tccgcctcca
     7441 tgccgagttc cgcccggatc agggagcgaa aatcctcgaa actctggcag aaatcaagaa
     7501 actggttgcc gtagtaccgt ctccatcctg gggccgtttc ccgcacgctt tcagccatat
     7561 tttcgccggt ggttatgccg caggttacta cagctacctg tgggctgacg tactggcggc
     7621 agatgctttc tcgcgctttg aggaagaggg cattttcaac cgtgaaaccg ggcagtcgtt
     7681 cctcgacaac attctgagcc gtggcggttc agaagagccg atggatctgt tcaaacgctt
     7741 ccgtggtcgt gaaccgcagc tggatgcgat gctggagcat tacggcatta agggctgatc
     7801 attcagtgaa aatctgctta attgatgaaa caggcaccgg agacggtgcc ttatctgttc
     7861 tggcggcccg ctgggggctg gagcacgatg aagacaacct gatggcgctg gtgttaacgc
     7921 cggaacatct ggaattgcgc aagcgtgatg agccaaaact tggcggcatc tttgttgatt
     7981 ttgttggcgg agcgatggcg caccgacgca aattcggcgg tggtcgcggt gaggcggtgg
     8041 cgaaagcggt gggcattaaa ggcgattatt tgccggatgt ggtggacgct accgcagggc
     8101 tggggcgcga tgcctttgtg ctggcttcgg tcggctgccg cgtgcggatg ctggagcgta
     8161 atccagtcgt tgccgcgcta ctcgacgacg gcctggcgcg tggatatgcg gatgcggaaa
     8221 tcggcggctg gttgcaggag cgtttgcagt taattcacgc ctccagcctg acggcgctga
     8281 ccgatattac cccgcgtccg caggtggtct atctcgaccc gatgttcccg cataagcaga
     8341 aaagcgcgct ggtgaaaaaa gagatgcgcg tctttcagtc gctggtggga ccggatcttg
     8401 atgccgatgg attactggag cctgcacgct tgctggcgac caaacgcgtg gtggtaaagc
     8461 gcccggacta cgcgccaccg ctagcgaatg tcgccacgcc aaacgcggtt gtgactaaag
     8521 ggcatcgctt tgatatctat gcaggtacgc cagtgtaaaa gtaaagcagg gtagcgataa
     8581 cgctaccctg ctttctgcaa gaataattaa tggctttccg gcgtcgcaat catgcgtttc
     8641 agccacggca ccatcaacag catcactact gcaacgccca gcgtgaccag accaatctta
     8701 ccaaacacgt tggtatagac aggcaacgtc tcaagcggat cggtaatgtt gtctggtact
     8761 gcggtaaatg ttgccacata gccgcccagc aagaacgcgg cagcctgtgt caggaaccac
     8821 atcccgagaa taaagcccat caaatgctgc ggcaccagag cagcaatcat cgccaggcca
     8881 agggcgctaa taaacagttc acctaagctc tggaataagt acaccagcac gataaaccat
     8941 ggcgatgtca gcccttgtgc atccgcaaac cacattcccg cagctgccgc cgtcaaaaag
     9001 cccagtgagc acataaacat gccgagagta aatttcatcg gcatcgagag gtctttgcct
     9061 ttgttaccca gatgcgtgta aatgcctgcc agtattgggc tggcgagtac cacccagaac
     9121 gggttaagcg cctggaagct gaccgggttg atggaaaaac cgagaatttc atgatgcacg
     9181 ttgttgatgg caaagaagtt cagcgatgtt ggcatctggg cgtagagaat gtaaaacacc
     9241 accgcttcga gcatcaggac aaaggcgaca aacattttat tgcgcccggt tttatccagc
     9301 ttgaatgcct gacgaaagaa gatgatggtg acgacgatgg agagaacaat cagcaccaga
     9361 ttggcgactt ctacgttgtg catcagccag gcgcagacga agatcatcac cacgctgcca
     9421 agtaatacgt acaacagttt gctgaagctc attggccgga agtcgggctc agaaccaatg
     9481 tctttcacca ttccacgaca ggcgatgtaa accagtaatg cgataattaa ccccgcaccg
     9541 cacaggttgt aggtgactga atagccgaat ttatcagcga tcacaggggc cagcgataac
     9601 gctatcaacg agccgatatt gatcgacata tagaacaggg tgaatgtgcc atcaaggcgc
     9661 ggatctttcg gcggatagca cttcgaaagc aagctggctg ggttagcttt aaacaggccg
     9721 ttaccgacag cgatagtccc cagggcgatg aaaatcaggt caggcttaag tagcgacatg
     9781 ccggtcatga agtagccaat cgccagcaca agtgctccaa gaacgatggt gcgtttggtc
     9841 cccagcaggt ggtcgccgac atagccgcca atggaaatga ggccatagac cagtgcagca
     9901 aaagcaccaa aagtgacaaa agcctgctct tgcgagaatc caagctgttt aacgaagaaa
     9961 accgccagta cgccctgcac gccgtagtag ccgaatcgct cccataactc gacaaaaaag
    10021 atcatgaaaa atgggcgagg ttgctgcagc atccccatgg gtgttgttgt attcatattt
    10081 attaaccttc caataccatc ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag
    10141 tttacatctg tcagagattc ttaatattga tgcggtgaat agattaatac actattacaa
    10201 cagaaaataa ccagctgatc acactaatga tggaaaatat gatggtgtta aatattgtat
    10261 tggctgggga ttttgattgc gaacgcagac cgtaggccag ataaggcgtt tacgctgatc
    10321 aggtaacaag gcgcgacaaa aagcaaaacg cccgcatatg cgggcgttca ggaacacgtc
    10381 gtggagggaa gattattctt cttcgtcgcg cagcggaaca atcagcatat caacgtgaac
    10441 ggtgttgatc agctgacgtg cggaagacat cagtttgctc cagaagtcct ggtggtgacc
    10501 acaaaccact agatccatat cgtatttctt gattgcatcg accagaacct ggcccaggtc
    10561 gccgctgccg ctcagggttt cagtgattgg gtagcctgca ttagtggaaa gctcggtcag
    10621 cgcatgatgt gtctcttcag agatgcgttt ctgcatatca ccaagattca catcaataag
    10681 cccggtatat aggtcagagt agtttacatc tacgtggatc agagaaactt tcgcattgta
    10741 tgggcgagcc atagagactg ctttctctac cagaactttg ctttccgggg agagatcgac
    10801 cgcgatgaga atgtgtttat aagccatagt gttactcctt ccataaagtt gtcgatgact
    10861 ggccagctag cgtttcttgt gccgttacgt cacccgcgtc ctgcgaacta cgtgcgcggg
    10921 gctaacccag ccgtaaagat tcagtgttaa gaccatcctt accttatagc gacccggatg
    10981 atccgtcaat ccgccttgct tacccgttag ataaacaaat tttcgacatg aatatattga
    11041 tagtggttaa ccttctggaa aaaaaacaac ctgatctcct acactatcta tagagccgct
    11101 cggatgttgg tcaccacaaa agcggtttct ccaggacctg gtgttaactg attggcagcg
    11161 tatcgggagc ggtagccccg gggtggaact ccgcgggcag gtcgccgggg aggaggtatg
    11221 ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa catggcgcgc
    11281 tatttctcat cactacgcgc gttgttagtg gtactgcgta actgcgatcc attgctctat
    11341 caatatgttg atggaggggg cttttttacc tcacatggcc aacccaacaa acaggtgcgt
    11401 ctcgtttggt atatctatgc ccaacgttat cgcgatcatc acgatgatga gtttattcgc
    11461 cgctgtgagc gggtgcgtcg gcagtttatt ctgaccagcg cattgtgtgg tctggtggtg
    11521 gtcagcctga ttgccttgat gatttggcat taagcacttc agcaataata acgcataaaa
    11581 aaagcgggcc agtaaactga cccgctcgtt ttggtgcgta ctattacagg aactgcaagg
    11641 agagccagta aagcccgccg gaaagcagta ccgcagccgg aagggtaaac acccaggcca
    11701 tcagaatgct ggtcacggtt ttacgctgta agccgccacc atctaccacc atcgtccccg
    11761 cgacagaaga ggagagtacg tgagtagtgg aaaccggcat cccggtataa ctcgccaggc
    11821 cgatagacac tgccgcagtc atctgggcag acatcccctg agcgtaggtc atgcctttct
    11881 taccgatttt ctcaccgata gtcgttgcca cacgacgcca gccaatcatc gtaccgatac
    11941 ctaacgccag cgcgaccgcc atgatgatcc acaccggtgc atactcgatg gtgctaagca
    12001 tgtcggactt cagtttcttc aacaggcgct gatcgtcagc actcacgcca ggcatcttca
    12061 ccactttgtc gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg ctacgttgat
    12121 caagcgacag cttgtcgtag ctttccacat cggtggtcaa catacctttc aggcggttga
    12181 gcgcgttaat ggtattcgac ggatggcagt ggaactccgc aggttgcgtt gcgccagctt
    12241 ccggagccgg tactaactga tcagcaccag tagcttgttt cagcagcgca ggatgctgct
    12301 caaagtaagc ttcgacgttg ttgatggcat cacgggtacg ggtgatttcg tagccagtgg
    12361 cattcatgtt caccacgaag cctgctggtg cgacaccaat caataccaac ataaccagac
    12421 caatgccttt ctgaccatcg tttgcgccgt gcgaaaacgc cacgccgata gcggaaagga
    12481 tcagcgcgat acgcgtccag aacggcggct ttttcttgcc gtctttcttt tcacgctccg
    12541 ctggggtcag gtggatacgg gcgcgtttct tggtgccgct ccagtagcga cgcagcaaga
    12601 aaatcagacc gccagcaaac accaggccga caatagggga aacgatcaga gaaccgaaaa
    12661 tacttaatac tttcgggata ttgagtgcat ccaccactga cgtcccggtc atcaacgcat
    12721 tggttaaacc aatcccgatg atcgcgccaa tcagcgtatg agagctggat gcaggcaaac
    12781 caaagtacca ggtacccagg ttccagataa tcgccgccag caacatagag aacaccatgg
    12841 caaggccatg agacgatccc atattaagca gcagatccgt cggcagcata tgcacaatgg
    12901 cataggcaac actcagacca cccagcaaaa cacccaaaaa gttgaacacc gctgccataa
    12961 ccacggcgag ctgagaacgc atcgcgcggg tatagataac ggttgccacg gcgttggctg
    13021 tgtcatggaa accattgatg gcttcgtaga acagcacaaa agccagtgca agcaataata
    13081 acagcccggt atgcaaatcc aggccagcaa acaaatgtag cataggacgt tacgccattt
    13141 tgaggacatg aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa gtgaaatata
    13201 gacttttttt gatttgcctc ctgtatggat ttcactcaaa aaataattat cttatataat
    13261 tcaggcaaat acttcctttt agtaatattg atgctggtgc gaccactgag gaatctttac
    13321 aattcacgcc cgttttttct aagaggagcg caacgtggaa aggtttgatg ccattattat
    13381 aggcgctggt gcggcgggta tgttctgttc tgcgctggca ggtcaggcag gacgccgggt
    13441 tctgctgatc gataatggta aaaaaccagg gcgcaaaatc cttatgtctg gcggtgggcg
    13501 ctgcaacttt accaaccttt atgtcgaacc gggcgcttat ctgagccaga atccgcattt
    13561 ttgtaagtct gcactcgcgc gttttaccca gtgggatttc attgatctgg tcaataaaca
    13621 cggcatcgcc tggcacgaga aaacgttagg gcaactcttc tgcgatgact ccgcgcagca
    13681 gattgtcgac atgctggtgg atgagtgcga gaagggcaat gtgaccgtca gattgcgtag
    13741 cgaagtgctg agtgtggcga aggatgatac aggcttcacg cttgaactga acggcatgac
    13801 tgtcggttgt gaaaagctgg tcatcgcgac cggcgggctg tcaatgccgg gcctgggcgc
    13861 gtcgccgttt ggttataaga ttgccgaaca atttggcctc aacgtgctgc cgacccgcgc
    13921 gggtctggtg ccattcactc tgcataaacc gttgctcgaa gagttacagg tgctggcggg
    13981 cgtggcggtg ccttccgtga ttaccgctga aaacggcacc gttttccgtg agaacttact
    14041 cttcacccat cgcggcttgt ctggaccggc ggtgttgcaa atttccagct actggcaacc
    14101 gggggaattt gtcagtatca atctgctacc ggatgtggac ctcgaaacct tcctgaatga
    14161 gcagcgtaac gcacatccga atcagagcct gaaaaacaca ctggcggttc atctaccgaa
    14221 gcggttggtt gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc taaaacagct
    14281 caacgtgcgt gaccaacagg cactgattag cacattgacc gactggcgcg tacaacccaa
    14341 cggcactgaa ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca ccaacgaact
    14401 ctcttcacgg acgatggaag cgcgcaaagt gcctgggctg tactttatcg gcgaagtgat
    14461 ggacgttacc ggctggctgg ggggctataa cttccagtgg gcgtggtcga gtgcgtgggc
    14521 ttgtgcgcag gatttgattg cagcgaagtc gtgctgagct atttttccta aagatgaaaa
    14581 ttattagagt cgcattaaaa atgagctaat tttgatagtg gctatcttgt gattattttc
    14641 taatgagccc gtgaactgaa ccccccatgc ttaatataag gtggagggaa aggtgattga
    14701 aaactcaccc agtgggctga tctttataaa atatgaacag gttgaaaaaa aaacagtaac
    14761 tttgctgttt tttatactta accactatgc attaatgctg cgttatttca ttctacctaa
    14821 gaaaaaccag cgttacgcaa atggttaacg ctggttttat ccggtacgtt gcaattattt
    14881 tttagcagaa cccgcttcta atattgaaac gattgagaac aacgtaaagc ataccgctcc
    14941 caaaccgacc agaacccgcg aaggaataaa atatcccata tcggtctgcg ccatttccgc
    15001 aagaaacgat gctaggaaaa ggcaaaacag gcaggtgaag acgggaatca tcggtattct
    15061 gttggctaac gaacaggtac gtctccagac cagtgccagt agccagactt ttgagaatat
    15121 gctgtaacag atcattccca agcaaatgag gataataccg ggagccagtc gggcgcttgc
    15181 atcagaactg actaagacgt atatgccctg cagcaccgtg attgagccga gaaaaataac
    15241 ccaataacac cacagccagt gctctttcgt tgaaaacgta ttacgtgttt gatggacgat
    15301 tgtggcaaca aggccaatta gacaggcaca gattgcggtt aacccgagca atacgtgacc
    15361 ggcgacataa tgcggagtta tgtcggcact acgtaacagc gtaatagacc aaatgaaacc
    15421 gagcagggtg agtaacacgg gaacggcaat gaggcaatta ccgattaatg aagaataagc
    15481 ctgtaccggt gttccgtcgc tcttgctccc cgctgcgttt ttaggaatga gcagaaagtg
    15541 accggatgac gctgccaccg tcgatacaca ggcggcaatc ataccaacgc cgaaaataac
    15601 atggccggcg acaaactcgt ctgccatcac atcattacct gctaacagtg cccaacccca
    15661 tatcatggtg ataattgacc ccgcatagcc aataatgggg aacaatgtat tgtaaaacgt
    15721 attaacgccg cgcgtgagct gcgaaatgat aataaatgcg gtagtgaata atgccaggca
    15781 tatggctgcc agagaaatga gcacatgacc cgcgacgaaa tactcgcttt gccctgaact
    15841 ttccagcaca aagccaccta acgcgataca aatcaagccc ataattaaag ggattaattt
    15901 gaaaagccac ccgatatata tgttcacttt aaactcctca ggtatccctg ctaatattga
    15961 aatgtgcatt aatgataatt tcagcctgtt aatacaatga cgagatatca ttaaagacaa
    16021 atgcatagca taactatagc ggggtttact ttgcagagcg ctacttttca gcgttcaaaa
    16081 ggccatgata aataaatatt tatcctaaga ttaattctta ttctgttgtt ttttacaatt
    16141 atcttccagg gagggattct ttttcattat ttggtaaaag accggatggt ctcggtgctt
    16201 aaaatcagcg agtatatacg catcattgc
//
LOCUS       DAZKFZ010000098        16027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000098 DAZKFZ010000000
VERSION     DAZKFZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..697
                     /locus_tag="ACHOQW_002786"
     CDS             <1..697
                     /locus_tag="ACHOQW_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9573841.1"
                     /translation="EQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWGIN
                     NRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTA
                     MCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMHYFSA
                     YSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQ
                     PECPVTLKDVSDL"
     gene            694..2145
                     /gene="djlC"
                     /locus_tag="ACHOQW_002787"
                     /pseudo
     CDS             694..2145
                     /gene="djlC"
                     /locus_tag="ACHOQW_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(2205..3875)
                     /gene="hscC"
                     /locus_tag="ACHOQW_002788"
     CDS             complement(2205..3875)
                     /gene="hscC"
                     /locus_tag="ACHOQW_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP9573842.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(3959..4894)
                     /gene="rihA"
                     /locus_tag="ACHOQW_002789"
     CDS             complement(3959..4894)
                     /gene="rihA"
                     /locus_tag="ACHOQW_002789"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP9573843.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5012..5737)
                     /gene="gltL"
                     /locus_tag="ACHOQW_002790"
     CDS             complement(5012..5737)
                     /gene="gltL"
                     /locus_tag="ACHOQW_002790"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP9573844.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5737..6411)
                     /gene="gltK"
                     /locus_tag="ACHOQW_002791"
     CDS             complement(5737..6411)
                     /gene="gltK"
                     /locus_tag="ACHOQW_002791"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP9573845.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6411..7151)
                     /gene="gltJ"
                     /locus_tag="ACHOQW_002792"
     CDS             complement(6411..7151)
                     /gene="gltJ"
                     /locus_tag="ACHOQW_002792"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP9573846.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7321..8229)
                     /gene="gltI"
                     /locus_tag="ACHOQW_002793"
     CDS             complement(7321..8229)
                     /gene="gltI"
                     /locus_tag="ACHOQW_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP9573847.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(8626..10164)
                     /gene="lnt"
                     /locus_tag="ACHOQW_002794"
     CDS             complement(8626..10164)
                     /gene="lnt"
                     /locus_tag="ACHOQW_002794"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP9573848.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10189..11067)
                     /gene="corC"
                     /locus_tag="ACHOQW_002795"
     CDS             complement(10189..11067)
                     /gene="corC"
                     /locus_tag="ACHOQW_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP9573849.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11157..11624)
                     /gene="ybeY"
                     /locus_tag="ACHOQW_002796"
     CDS             complement(11157..11624)
                     /gene="ybeY"
                     /locus_tag="ACHOQW_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9573850.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11621..12661)
                     /locus_tag="ACHOQW_002797"
     CDS             complement(11621..12661)
                     /locus_tag="ACHOQW_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9573851.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12814..14238)
                     /gene="miaB"
                     /locus_tag="ACHOQW_002798"
     CDS             complement(12814..14238)
                     /gene="miaB"
                     /locus_tag="ACHOQW_002798"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP9573852.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14384..15559
                     /gene="ubiF"
                     /locus_tag="ACHOQW_002799"
     CDS             14384..15559
                     /gene="ubiF"
                     /locus_tag="ACHOQW_002799"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP9573853.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(15713..15787)
                     /locus_tag="ACHOQW_002800"
     tRNA            complement(15713..15787)
                     /locus_tag="ACHOQW_002800"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15753..15755),aa:Gln,seq:ctg)
     gene            complement(15825..15899)
                     /locus_tag="ACHOQW_002801"
     tRNA            complement(15825..15899)
                     /locus_tag="ACHOQW_002801"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15865..15867),aa:Gln,seq:ctg)
     gene            complement(15948..16024)
                     /locus_tag="ACHOQW_002802"
     tRNA            complement(15948..16024)
                     /locus_tag="ACHOQW_002802"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15988..15990),aa:Met,seq:cat)
BASE COUNT         3881 a         4085 c         4163 g         3898 t
ORIGIN      
        1 agaacaatat cttctttttg cgctttctgc gcccatggaa atacttaatc agggttgcaa
       61 acccgcgcat gactcgccaa aaatgtatac cgggataaaa gaattcgatc tctcatcatc
      121 ctggggaata aataatcgtg atgatttaat tcagactatt tatcagatga ctgatgatgg
      181 tcatgccaat gatttagctg gcttatatct gacatggcat cgctcttccc ctgaagagtg
      241 gaaagcatta attgctggtg gctcggagag agggttgatt tatacccaat tcgttgccca
      301 aaccgctatg tgttgtgggg aagggggaat caaagcttgg gattatgttc gaatggggtt
      361 tctgtcacgg gtcggtgtgc tcaacaagtg gctgacggaa gacgagagtt tatggctgca
      421 atcgcgcgtc tatgaacgag ctcaccatta ctatcacagc tggatgcact atttttccgc
      481 ctattcacta gggcgtctct actggcaatc ttctcaatgc gaagacaacg cttcgctacg
      541 cgaggcgcta accctgtaca aatacgatag cgccgggagt cggatgttcg aagagctggc
      601 tgcgggaagc gatcgtttct atgccacgct tccctggcag ccattaactg tccagcctga
      661 atgcccggta acgcttaagg atgtgagcga cctatgaaaa catgttggca aatcctcgaa
      721 attgaaagca cgacgcaaat agacattatc cgccaggctt atcttgctcg cttaccgtta
      781 tgtcatcccg aaaccgatcc gcaagggttt aaagcattac gccaggccta tgaagaggcc
      841 ctgcgactgg cggtaaatcc tgtcgaggaa gcagatgatg aagaaaaaga tgatgccact
      901 gagcatgaaa tactacgtgt attcagggca ttactggatt cagaaagtga tcgttttcag
      961 ccctccgcct ggcagaaatt tattcagcaa ttaaatacct ggaacatgga ggatgtcgat
     1021 caattacgct ggccgctgtg tgcaatcgct atagaagcgc gatatctttc attaaattgt
     1081 gcttctttgc tggcagagcg tttgaactgg cattcgttta atgacagcga aggaatagat
     1141 gagtaagaaa gggaggcttt tcttgaggcc attcaggctg gtgattgttt cgatttcctt
     1201 ggccttctgg tatatcccgt tgcgttgcag aaccagactg ttgagtatta cttcgcgctg
     1261 gaacgttgct gccgttacca tcctgactat gtcactgcgt ttttggcgat ggaaggtccg
     1321 tggttcattc ctgatgatgc aaagttacat cgcaaactgt tgcgctggta cagctcggtg
     1381 caaacaggta tggcggaact cattcctgta gccgaacagt ggcaaatgga agaaccagaa
     1441 agcgaagatg cccggtatta ccagtgtgca caacgtttgt actgcggcga gggggaaagc
     1501 ctgcttaccg atctctgcgc gtactgggga agttacccat ctacacaagc cgataatttg
     1561 ttgttgcagt ggagcgcaca acattgcccg gattatttcg cgttattagt gatggttatc
     1621 gaagcgcgga gcatggtaga tgcgcaaggt aaaccgttga aatatgttcc tggtgagagc
     1681 gcccggacgc gtctgttatg ggcggaaatt ttacatagcg gaaaactatc gccgttaggt
     1741 caatcgttta ttgagtcgct attcttcaaa cgcaaagcat gggcgtggtg gaaatcgaga
     1801 gtcggtagcg agacagaaga agattcaccg ttactggatt tgtatcgggt agcggaacag
     1861 gtagtacttg aagcgtttcc gaaacaagag atgctggccc gtcttaatac aaggctggaa
     1921 ggcggagatg ctcatccatt agaggccatt atcacccgga tgctgttgac gaaagtaaaa
     1981 ctcgagccgg aggatgaaga tgtcgatgag ccaacacctg aaaaccatga agaaaaaaat
     2041 gatgaggatg aaaaaccaca gagcattacc agcattatca aaatcagctt aacggtgctg
     2101 gtgataggtt atgctctcgg caaaatcgcg atgttgttta gttgacgatg tgttttttgc
     2161 gccgatgccg gatgcagcat ttacgccgca tccggcagcc gtgattatgg gatctcaatg
     2221 gctaaataaa cgctcgcctg ccgcctgacc tcatcgacgc gggccatatc gtttgactgc
     2281 agcgccgcat caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc acccagcgcc
     2341 cgcgcccaca actcttccag tttggcctta aaggtgcggt tgatgagcat atcgcgcgga
     2401 taaattttca atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc gctcaatgtt
     2461 accgggctgt ggttaatcac tctggactta acgctgccgt cttccagaag cacgtcaact
     2521 tcaagcaaac cgttgatatc ataactaaag cgaatatcaa tcgactgata agccccggtt
     2581 ctcttcaacg gcacatcgaa ggattccacc agaatgttgt ttttaacttt gtggttttct
     2641 ccctgataga cgttaaccgt aatagaatcc tgttccgggt gcatggttga ataagtttct
     2701 acacgcgaca cgggcacagt ggtgtttcgt tcaataatcg gcgagaaaat gccggaaacg
     2761 ccctggcggt taacctcaac gcccaacgag taagggcaaa tatcagtgag gatcacttct
     2821 tcaatatctt cactgcgtaa gcggcaggct gcctggattg ctgcgcccag cgcgacaatg
     2881 gtgctcggat cgtaactttg atacggtaat ttgccaaaca gacgcacggc gattcgctgc
     2941 accagcggca tttgtgacgc gccgccaacc agcaccagac tgtcgatttg actcggcttc
     3001 agacgcgcat cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag cagcggcaac
     3061 cacaaatctt ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcatc ctggtactgc
     3121 cagcgaatgt gtattggcga ttgattgcta cattttgccg cttccacaca ggcatacaag
     3181 gctgccagtt cactctcgtt aagcgtggtc ctggcgacat ccgcgcgttt taaaacctca
     3241 tcgaccagca tatgggtaaa atcttcgcca ccgaggaagt tgtcgccagc ggaggcgtga
     3301 acttcaatca ccggcgtggc gtactcaagc accgtgacgt caaacgtgcc gccaccgaga
     3361 tcaaacacca gcgaacgggt attttgttgg gtatgcaggc catacgccat cgcagctgct
     3421 gtgggttcat taattaagcg taccgcattt aaaccggcta actccgctgc taaacgggta
     3481 tgcttgcgtt gttcatcgct gaaataagcc ggaacagaga tcaccacatc tttaatcgga
     3541 cgttgcagaa attcttcggc atcttctttt aatgagcgta ataccaaaga ggacagttct
     3601 ggcgcgttaa atgtgtcgct gcctaaccgc cagttggtat tactgcccat tgcacgttta
     3661 aataacgctg ccgttttatc cggatgcgaa gtacgccgtg atacagccgg ttttccgact
     3721 aaaatatgat tattttcatc catgctaatt atggatggtg ttaaatattc accgaactta
     3781 tttggaatta attgcgcggc accgtctttc cagacggcaa ttaaactatt ggtagtaccg
     3841 agatcgatac caatggcgag ttctgcatta tccattgcat ttatatcttg aaagaaaaag
     3901 agataaacag attaagacca cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt
     3961 aagcgtaaaa tttcagacga tcagccagta aatcaacaaa gccctgacga tcaacatcga
     4021 ccattacagt ggcattcggt ttattgccag tcagataata ataatcaaca accgtcatac
     4081 cctgggtata tttcccctgt gtttccacgc caacccagcg ctcaacagtg gtaaacaact
     4141 ccggttttaa cagccaggcg atggtgcatg ggtcatgtag cggtgcgccg acaaagcccc
     4201 atttttcgtc tttatgatat tcgaggaaga aatccagcag ttcggcaaca atggttgaaa
     4261 cagggttgcc aatcgcgcgg aaacgctcag tgtcttcaac gtggatttgt gctttgtgag
     4321 taacatccag accggccatc accaccggga tccctgactg gaagacaatt tctgccgctt
     4381 ccgggtcaac gtaaatatta aattcagccg caggcgtcca gttacccagt cccatagcgc
     4441 cacccataat cacgatacgg gcaattttgc tatgcagttc cggatggcta ttgagcagca
     4501 aggcaacgtt agtttgcggt ccggtagaca caatcgtgac aggttccgca ctttcacgca
     4561 gcgttttcgc catcagctct accgccgtac agttttgcgg tgcgaacgtc ggttccggta
     4621 atgccgggcc gtcgagaccg ctttcgccgt gcacattgtc cgcgataatc aactcacgca
     4681 ttaacggttt taccgcgccg cctgccaccg gaatatcggt gcgattaagc aaggtcagca
     4741 tacgcagaac attgcgtaag gttttttctg gtgtctggtt tccggcggaa gacgtaattg
     4801 ctttgacatc aagctctggt gaggcgaggg cgagaactat tgcgatagcg tcatcatgac
     4861 ctgggtcgca atctaacaga attggcagtg ccattgttgc tccttgttgt gtgcttcttt
     4921 gcgacaaggg taacgccagg atgtaacaga tacgaggggc gagacgataa agcgtgagat
     4981 ggcgcgcact tgggtatgcg cgccagagtg attaatgcag gattttcgcg aggaagtctt
     5041 ttgcgcggtc cgatttcgga tcatcgaaga acgcatcttt cggcgagtct tcaacaattt
     5101 taccctcatc cataaagatc acccgattcg ccactttacg ggcaaagccc atttcgtggg
     5161 tcaccaccat catggtcatc ccctcgttcg ccagttccac catcacgtcc agtacttcgt
     5221 tgatcatctc cgggtctagt gccgatgtgg gttcgtcgaa cagcatcgca ataggatcca
     5281 tacacaatgc gcgagcaatt gccacacgct gctgctggcc gccggaaagc tgcgccggaa
     5341 atttattggc atgtgcggaa agtcccacgc gctccagcaa tttcaatgct ttttcacgag
     5401 ccggtgcttt atcgcgttta agcactttca cctgcgccag ggtcaggttt tcgataatcg
     5461 acagatgagg gaacagctcg aaatgttgga ataccatccc gacgcgagaa cgcagctttg
     5521 ccagatcggt tttcttgtcg ttaaccacga taccatcgac ggtgatttca ccttgctgca
     5581 ctggttcgag gccgttgacg gttttaatca gcgttgattt gccggaacca gacgggccgc
     5641 aaaccaccac cacttcgcct tttttcactt cggttgagca gtcggtcagc acctgaaagt
     5701 gaccatacca ttttgaaaca tttttcaggg taatcattat gctgtccttc ttttcaagta
     5761 gctgaccaac aacgacgcgc taaggctaat aacgaaataa acaaatccgg caaacaggat
     5821 catctcaacc tgcgtgccat cacgctcacc aatggttgag gcggtacgga agaaatcggc
     5881 cagagataac acatacacca gcgaggtatc ctggaacagt acgatgccct gagtgagcag
     5941 cagcggcacc atcgcgcgga acgcctgcgg cagaataatc agtttcatcg actgccagtg
     6001 agtcattccc aacgccagcg cggcgctcga ctgaccacga gaaatacttt gaataccggc
     6061 acggataatc tctgaatagt aggccgcttc aaacatcgaa aacgccacca tcgccgaaat
     6121 taaacggata tcattttttg gtgataatcc cagcacgttt tgcagaaaac ccggcacgat
     6181 caggtaaaac cacagcaaaa ccataactaa aggaatcgag cggaatacgt taacgtaggc
     6241 tttggcaaac cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc
     6301 ccacaaaata ccaatcacga ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc
     6361 gagcagatat ggcagggaag ggacaatgga actccagtca aactcgtaca ttatttgccc
     6421 cccatgttgc caggcaggcg aactttacgt tcaaccagcg tcatcaccag catgataaaa
     6481 gcgttaataa acacatacgc tagcgtaatg gcggtaaacg actcccaggc gtgagccgag
     6541 taatcgagca atttactcgc ctgcgctgcc atatctacca gaccgatggt cgaggcgatg
     6601 gcggagtttt tcaccaggtt catcatctct gaggtcatcg gcgggacgat aacgcgataa
     6661 gcattaggca gcagtacgta gcggtaagcc tgcggcagcg tcagacccat agccagcgcg
     6721 gcattcttct gcccacgcgg cagcgactga atcgcggcgc gtacctgttc gcaaacacgg
     6781 gcggcggtaa acagccccag gcagagcatg gatgaaagga aaaactgaat attgggatcc
     6841 agctcggcct taaaccacat gccgattttc tccggcagca gctccgggat caccagatac
     6901 caggtaaaga attgcacaat caacggcacg ttgcggaaca gttcgacata caacgtaccc
     6961 agaccggaga gaaaacggtt tggaacggta cgtaaaatac cgaaaaatga accgacgagg
     7021 aaagcgataa tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc actccagatc
     7081 caaccaagat aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc ccagttccag
     7141 tctatagaca taaatctact ccagaaaaaa gagggtagca gcgttaactg ctaccctcga
     7201 agattgttac ccagcgtatt gcggttttca ggcccgatgg ggaacgacca tcaggcgtat
     7261 agtctgtccg tgctacgtaa caatcgagag ggctagaatt tccgcccctg gttcttgtaa
     7321 ttagttcagt gctttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc
     7381 gaaattcatg ttcaggtttt tcggcggaat gggatttttg aaccatttat caaaccattt
     7441 ttccgcttca ccggaggtct gcacctgagc gatagtgtca tccatcagct ttttgaactg
     7501 cggatcgtct ttacgcaaca tgcaaccata ggcttcctga gactgcggct tgccgacgat
     7561 atcccagttg tctggtttct tcgctttcgc acgttcaccg gccagcagag catcatccat
     7621 cataaaggca acggcacgac cgctttccag ggtacggaaa gagtcaccgt gatctttggc
     7681 gctgatgatg cgcatattca ttttttgctc ttcattcagt ttgttgagca aaacttcaga
     7741 ggtagtgccg gaagtgacga ctacggcttt gcctttcagg tcggcaaaat ctttgatatc
     7801 gccgcccttt ttggtcaaca ggcgcgtacc gaccacgaaa atagtgtcag agaaagccgc
     7861 ctgtttttgg cgttcgacgt tgttggtggt agaaccacat tcaaaatcaa aagtgccgtt
     7921 ttgcagcagc ggaatacggt tttgtgaggt aatcggaatc agtttcacct gcaagtccgg
     7981 tttgttgagt ttctttttca ctgcttcaac aatggcgttg gagtaatcct gcgaataacc
     8041 caccactttt tgctgattgt cgtaataaga gaaaggtact gaagattcac ggtgaccgac
     8101 gacaatcacg ccgtttttgg cgattttgtc cagcgtactg cccgctgccg gggcggcgtc
     8161 atctgcctgt gccagtcctg cggaaagcgc cagggccagg attgctgtgg caggtttacg
     8221 taattgcata tccaactcct ttatcttctg cgttaagaac gcatggatac ccgttgtgag
     8281 tgtttgtgtt gttatcatct gcaactttat tgtgtagtgt tgtgcctgtt aggtaacatt
     8341 tagtttggct aaatgtaaag atattgctgt tttattgttt atatttggga ggtgcgccgc
     8401 accattccga agcaaaattc ttaaaatgca ctcttttggt gctaccggtg gattgctgtg
     8461 gtgcaaccgg gttgtactga tgctggttaa agctttgcct tgtataacaa agcaatagat
     8521 gtgccaaagt tggataggag aatattgtta tccggataat gcactgatgc cgcatccggt
     8581 gagcgtgccg aaatatggga tgtattccgg cacgataaga agggattatt tacgtcgctg
     8641 acgcagactc atcaacacag cagcaaaacc aaacagtgcc gtcagcaccc acagcggcca
     8701 gttgccggta cgtgcgtatg gtgtgagtcc ggtggtcggc gtcacgttag tggttaacac
     8761 ctcgcgggtg aactgcggga tcatcgcctg aatctcaccc tgcgggccaa tcaccgccgt
     8821 aatgccgttg ttggtgctgc gcaacagtgg gcgcgccagc tccagcgcac gcattcgcgc
     8881 catctggaag tgctgccatg gaccaataga cttaccaaac cacgcatcgt tggagatagt
     8941 cagcagatag tcggtatccg ggcggaagtt atcgcgcact tgctcgccga gaatgatctc
     9001 gtagcaaata gccgcagtaa gctcaatacc atttgccgac agcggcggct ggatatatgg
     9061 cccacggctg aacgacgaca tcggcagatc aaagaacggt gctaacggac gcagaatcga
     9121 ctccagcggg acaaactcgc caaacggcac cagatggttt ttgttatagc gatcggctga
     9181 ttcgtagctg tacggtgcac ctttacccag tgtgatgatg gtgttgtagg tatcgtaacg
     9241 gttctgctta ttaagacgcg cgtcaacaat cccggtcacc agcgagctac ctttatcacg
     9301 caactcaccg tccagtgctt tgaggaacgg ttgctgatta atttccagat cggttatcgc
     9361 cgactccggc cagataatca acgatgattt gcccatcagc ggtgccgttg cgttgtagta
     9421 aatcttcagc gtattaagaa gctggccttc gtcccatttc agcgattgcg gaatatcgcc
     9481 ctgaaccatc gaaacctgaa tggttttctc cggttgtggg gtaaaccacc ggatgtaacg
     9541 cagcgggaag ggaagggcaa acagcacgac ggccaccacc agcggacgcc agttgcgttt
     9601 gactaacgcc agtgccagca ggccgctaac catcataagc aggaagttaa tggcttccac
     9661 gcccattagc ggtgccagcc cttttaacgg gccatcaatc tggctatagc cgaactgtaa
     9721 ccacgggaag cctgtcagta cccaaccgcg cagaaactcg gtcacttgcc agagagcagg
     9781 ggcggcaatc gccacgcgca gccaggtggt tttcggccac aggcgcgaca gcacgccagc
     9841 aaacagtccg gtatacagcg acaaatacgc cgccagcagt accaccagga agatgttaac
     9901 cgggccaggc attccgccaa aggtcgcgat gctgacatag acccagttaa taccgctgcc
     9961 aaagaggcca aatccccagc aaaagccaat agcggcagac tggagtggac ggcggttaaa
    10021 ggtcaacgcc tgaagcccca tcagcgaaat aatcgccgca ggccagacgt cgtaaggaga
    10081 gaaggccagc gttccgcagg caccgaataa taacgccagc agcaggcgaa tgcgctggcg
    10141 ttcaattaat gaggcaaaag ccatgtagtt atctatccag tttcggtttt attcatccag
    10201 cttcggctgg ggtgagtcat ccgggatttt gacatgaacc tgaataatac gccgactgtc
    10261 ggccatcgcc actttgaact ggtaaccgtc gatgtcgatg gtttcgccac gtgccggaag
    10321 atgcccaaat gcctgcatca ccagaccacc gatagtgtcg acttcttcat cgctaaagtg
    10381 ggtgccgaac gcttcgttga agtcttcaat ggaagccagt gcgcgcacgg tccaggtatg
    10441 acgactcagc tgacggaagt cgatatcatc ttcttcgtca tattcgtctt caatctcacc
    10501 aacaatcagt tccaggatgt cttcaatggt taccagaccg gaaaccccac cgaattcgtc
    10561 aataacgatc gccatgtggt aacgctgaga gcgaaactct ttcagcatcc ggtctacgcg
    10621 cttactttca ggaacgacaa ccgcctgacg taacactttg tccatgctga aggcttcagc
    10681 atcgctgcgc ataaacggca gcaagtcttt cgccatcaga atcccttcaa tgtgatcttt
    10741 gtcttcgcta atcaccggga aacgtgagtg ggcggactcg atgatgacat caaggcattc
    10801 gtccagcgtc tggttgcgtt tcagggtaat catctgggag cgggggatca tgatgtcgcg
    10861 gacgcgttgg tctgcgatgt ccatcacccc ttcgagcata tcgcgcgtat cttcgtcgat
    10921 aaggtcgttc tgcccggaat cacggatcag cgccagcagt tcgtcacggt ttttcggttc
    10981 accgtggaaa agttggctga gtaacaggga gaaaaatccc ttcttgttgc ttatcgtgtc
    11041 actactgtgt gaattgtcgt cgctcatggc gtcgtatggg ttctcatgtt agttaatcaa
    11101 aacgccgtcg ttaatcacca acggcgggga cgtctgccag tcaaatggct ggcaaattat
    11161 tctttctcgg caatgtacgg atcctcatag cccagagcaa gcataatctc tgtttcgagg
    11221 gcttccattt cttctgcttc gtcatcttcg atgtgatcgt aacctaacaa atgcagactg
    11281 ccgtgcacca ccatatgcgc ccagtgcgcc tccagtggtt tgccttgctc ctgagcttcc
    11341 ttctcaacca tctgacggca gataaccaga tcgcccagta gcgacatctc catgccaggc
    11401 ggcacttcaa acgggaagga gagcacgttg gtcggcttat ccttaccgcg ataggtcaga
    11461 ttgagactgt ggctttcggc ggtatcgacc acgcgaatcg tcacttccga ctcttcctga
    11521 aactgcggga tcaccgcatt cagccatgtt tgaaactggc tctcttccgg caatccggag
    11581 ttatcttcac atgccagttg taaatcgagg atcacctgac tcatttttgt tcctgttctt
    11641 cgcgcttgcg ttctgccgcc agcgccgctt tacgtttttg ttcggcttct tcccaggctt
    11701 cataggcgtt aacgatacgc gccaccaccg ggtgacgaac cacgtcttcg ctgtggaaga
    11761 agttaaagct gatctcttcg acatcggcca gcacttcgat ggcatgacgt aagcctgatt
    11821 tagtattacg cggcaggtcg atctgtgtga cgtcgccggt gataaccgct tttgagttaa
    11881 aaccgatacg ggtcaggaac atcttcatct gttcgatggt agtgttctgg ctctcatcga
    11941 gaatgataaa tgcgtcgttc agcgtacgac cacgcatata ggccagcggt gcgacttcaa
    12001 taacgttgcg ctcaatcagt ttctcgactt tctcaaaacc cagcatttca aacagcgcgt
    12061 cgtacagcgg gcgcagatac gggtctactt tctggcttaa atcgccaggc aggaagccca
    12121 gtttctcacc ggcttctact gccggacgag tcagcagaat acggcgaatt tcctgacgct
    12181 ccagggcatc aactgccgca gccactgcca ggtaggtttt acccgtaccc gccgggccaa
    12241 cgccgaaggt aatgtcatgg tcgagaatat tggcgatgta ctgcgcctgg tttggcgtgc
    12301 gcggcttaat tacgccgcgt ttggttttga tattgaccgc tttgccgtac tccggcacgc
    12361 tctccgcgct ttgctccagt acccgtgctt ctttaatcgc aaggtggatc tgttccggtt
    12421 cgatatcctg aatctgaccg cgcatcgggg cagtatcgac atacaggcta cgcagaatgt
    12481 ctgccgcagc ggtgacgcaa atcggacggc cggtcagttt aaagtggtta tcgcggcgat
    12541 tgatctcgat gccgagacgg cgttcgagct gcttgatgtt gtcatcaaac gggccgcaca
    12601 ggctcaacag acgcgcgttg tctgctggct ccagggtgat ttcgcgagtg tctatgttca
    12661 aaccgttcct cttatctgta tgccgccgga agctgaacat tcaccggcct ataaggaaat
    12721 tattcacgcc acaggaaaaa ggcgcaagca ttgcgataaa gatggggata aagagagaaa
    12781 aaacaaggcc caccggaacg gcgggcctga gaattacggc tgataataac ccacgccaag
    12841 gtcgttttct ttgcgagtac gggcaatcac cgattctggt gtttctgcca cgcgcagacc
    12901 catttcatct tcagtacgca ccactttacc gcgcagagag ttcgggtaga cgtcggtaat
    12961 ttctacatcg acgaatttac cgatcatatc cggcgtgcct tcgaagttga ccacgcggtt
    13021 attttccgta cgcccggaaa gctccatgat gctcttacgc gatgtacctt ctaccagaat
    13081 acgctgggtg gtgccgagca tccggcggct ccacgccatc gcttgctgat tgatacgttc
    13141 ttgcagaata tacagacgct gcttcttctc ttcttccgga acatcatcaa ccatatcggc
    13201 ggctggtgta cccggacgtg cagagaagat aaagctgtag ctcatgtcga aattgacgtc
    13261 ggcaatcagc ttcatcgttt tctcgaagtc ttcggtggtt tcgccaggga agccgacgat
    13321 gaagtcagaa ctgatctgaa tatctggacg cgccgcacgc agtttacgga tgatcgcttt
    13381 gtactccagc gccgtatggg tacgtcccat caggttcaga atgcgatcgg aaccgctctg
    13441 taccggcaga tgcaggaagc tcaccagctc cggcgtgtcg cgatacactt cgatgatatc
    13501 gtcggtgaat tcgatcggat ggctggtggt aaagcgaata cgatcgatcc cgtcgatcgc
    13561 agcaaccaga cgcaacagat cggcaaacga tccggtggtg ccgtcgtagt tttcaccacg
    13621 ccaggcgttc acgttctgac cgagcaggtt gacttcacgc acgccctgag ccgcaagctg
    13681 ggcaatctca aacagaatat cgtcagacgg acggcttacc tcttcaccac gggtgtaagg
    13741 caccacgcag taggtgcaat atttattgca gccttccatg atggagacaa acgcggtcgg
    13801 cccttcggcg cgcggttccg gtagacggtc aaacttctcg atttccggga agctgatatc
    13861 tacaaccggg ctgcggtcgc cgcgcacgga gttgatcatc tccggcagac ggtgcagcgt
    13921 ttgcggccca aaaataatat cgacatagtg ggcgcgctgg cgaatgtgct cgccttcttg
    13981 cgatgccacg cagccaccga cgccgataat caggtctgga ttcttctctt ttaacagttt
    14041 ccagcgaccc aactgatgga agactttttc ctgagccttc tcgcggattg agcaggtgtt
    14101 cagcagcagc acatccgctt cttccgccac gtcggtcagt tgatagccgt gggtggcatc
    14161 cagcagatcg gccatcttcg atgaatcgta ctcgttcatc tgacagcccc aggttttaat
    14221 atggagtttt ttggtcatcg acttgctctt gcgaaatagt agccaggaat gcagggcgca
    14281 tagtgtaatg ctttgctgcc gttgtgacca gtatgagcgt tatcagccct tgggggtaaa
    14341 aatcctgtaa acttaaagca tattgctaac aggatgattg accatgacaa atcaaccaac
    14401 ggaaattgcc attgtcggcg gaggaatggt cggcggcgca ctggcgctgg ggctggcaca
    14461 gcacggattt acggtaacgg tgatcgagca cgcagaacct gcaccgtttg tcgctgatag
    14521 ccaaccggac gtgcggatct cggcgatcag cgcggcttcg gtatcattgc ttaaagggtt
    14581 aggggtctgg gatgcagtac aggctatgcg ttgccatcct taccgcagac tggaaacgtg
    14641 ggagtgggaa acggcgcatg tggtatttga cgcagccgaa ttaaagttgc cgttgcttgg
    14701 ctatatggta gaaaacactg tcctgcaaca ggcattgtgg caggcgctgg aagcgcatcc
    14761 gaaagtaacg ttacgtgtgc caacctcgct gattgcatta catcgagata atgatcttca
    14821 ggagctggag ctgaaaggcg gtgaagtgat tcgcgcgaag ctggtgattg gtgccgacgg
    14881 cgcaaattcg caggtgcggc agatggcggg aattggcgtt catgcatggc agtatgcgca
    14941 gtcgtgcatg ttgattagcg tccagtgcga gaacgatccc ggcgatagca cctggcagca
    15001 atttaccccg gatggaccgc gcgcgtttct gccgctgttt gataactggg catcgctggt
    15061 gtggtacgac tctccggcgc gcattcgcca gttgcagaat atgaatatgg cgcagttaca
    15121 ggtggaaatc gcgaagcatt tcccgtcgcg tctgggttac gttacaccgc ttgccgctgg
    15181 tgcgtttccg ctgacacgcc gccatgcgtt gcagtatgtg cagccggggc ttgcgctggt
    15241 gggtgatgcc gcgcacacca tccatccgct ggcggggcag ggggtgaatc ttggttatcg
    15301 tgatgtcgat gccctgattg atgttctggt gaatgcccgc agctacggcg aagcgtgggc
    15361 cagttatcct gtcctcaagc gttaccagat gcggcgcatg gcggataact tcattatgca
    15421 gagcggtatg gatctgtttt atgccggatt cagtaataat ctgccgccgc tgcgttttgt
    15481 gcgtaatctt ggtttgatgg cggcggagcg tgctggcgtg ttgaaacgtc aggcgctgaa
    15541 atatgcgtta gggttgtagc cttacaacat tgccggatgc gtgccaaccg taggtcggat
    15601 aagacgcggc agcgtcgcat ccgacattga aggataagac gtgtcaacat cgcattcgac
    15661 attgagtgaa cgcagaaaag caaaaagctc gccgaagcga gcttttttaa tgtggctggg
    15721 gtacgaggat tcgaacctcg gaatgccgga atcagaatcc ggtgccttac cgcttggcga
    15781 taccccaact gggtgcactt acaaggtaag cgtcttgaat aaattggctg gggtacgagg
    15841 attcgaacct cggaatgccg gaatcagaat ccggtgcctt accgcttggc gataccccaa
    15901 caaattggtt tttgaatttg ccgaacgtat tcgacacatt cagaatttgg tggctacgac
    15961 gggattcgaa cctgtgaccc catcattatg agtgatgtgc tctaaccaac tgagctacgt
    16021 agccaga
//
LOCUS       DAZKFZ010000099        15620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000099 DAZKFZ010000000
VERSION     DAZKFZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(228..881)
                     /gene="ribB"
                     /locus_tag="ACHOQW_002803"
     CDS             complement(228..881)
                     /gene="ribB"
                     /locus_tag="ACHOQW_002803"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP9573854.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(985..1133)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1255..1554
                     /gene="ubiK"
                     /locus_tag="ACHOQW_002804"
     CDS             1255..1554
                     /gene="ubiK"
                     /locus_tag="ACHOQW_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP9573855.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(1589..1789)
                     /gene="glgS"
                     /locus_tag="ACHOQW_002805"
     CDS             complement(1589..1789)
                     /gene="glgS"
                     /locus_tag="ACHOQW_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP9573856.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            2058..2672
                     /gene="yqiJ"
                     /locus_tag="ACHOQW_002806"
     CDS             2058..2672
                     /gene="yqiJ"
                     /locus_tag="ACHOQW_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9573857.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            2714..4375
                     /gene="yqiK"
                     /locus_tag="ACHOQW_002807"
     CDS             2714..4375
                     /gene="yqiK"
                     /locus_tag="ACHOQW_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9573858.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            4614..4673
                     /locus_tag="ACHOQW_002808"
     CDS             4614..4673
                     /locus_tag="ACHOQW_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9573859.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            4990..5049
                     /gene="ibsE"
                     /locus_tag="ACHOQW_002809"
     CDS             4990..5049
                     /gene="ibsE"
                     /locus_tag="ACHOQW_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP9573860.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(5169..6602)
                     /gene="hldE"
                     /locus_tag="ACHOQW_002810"
     CDS             complement(5169..6602)
                     /gene="hldE"
                     /locus_tag="ACHOQW_002810"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP9573861.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6650..9490)
                     /gene="glnE"
                     /locus_tag="ACHOQW_002811"
     CDS             complement(6650..9490)
                     /gene="glnE"
                     /locus_tag="ACHOQW_002811"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP9573862.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(9513..10814)
                     /gene="ygiF"
                     /locus_tag="ACHOQW_002812"
     CDS             complement(9513..10814)
                     /gene="ygiF"
                     /locus_tag="ACHOQW_002812"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP9573863.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            11056..11676
                     /gene="ygiM"
                     /locus_tag="ACHOQW_002813"
     CDS             11056..11676
                     /gene="ygiM"
                     /locus_tag="ACHOQW_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP9573864.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            11740..12978
                     /gene="cca"
                     /locus_tag="ACHOQW_002814"
     CDS             11740..12978
                     /gene="cca"
                     /locus_tag="ACHOQW_002814"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP9573865.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(13159..13980)
                     /gene="bacA"
                     /locus_tag="ACHOQW_002815"
     CDS             complement(13159..13980)
                     /gene="bacA"
                     /locus_tag="ACHOQW_002815"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9573866.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(14071..14439)
                     /gene="folB"
                     /locus_tag="ACHOQW_002816"
     CDS             complement(14071..14439)
                     /gene="folB"
                     /locus_tag="ACHOQW_002816"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP9573867.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            14545..15162
                     /gene="plsY"
                     /locus_tag="ACHOQW_002817"
     CDS             14545..15162
                     /gene="plsY"
                     /locus_tag="ACHOQW_002817"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9573868.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(15175..>15620)
                     /gene="ttdR"
                     /locus_tag="ACHOQW_002818"
                     /gene_synonym="dan"
     CDS             complement(15175..>15620)
                     /gene="ttdR"
                     /locus_tag="ACHOQW_002818"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP9573869.1"
                     /translation="KNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWE
                     LGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEY
                     AQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
BASE COUNT         3862 a         4285 c         3831 g         3642 t
ORIGIN      
        1 ttagcgcata aatttcacat atcctttttc ggatatattc atatggtcgc ataataacgg
       61 aacaattata tgattttgat cataatgaaa aagaatgcct gatattaatg aaattttctc
      121 atagaaagag aagcggaatt aacatcttgc atagtaggga aaaataaaaa ccgaagcctg
      181 ctacaacttc ggtttcttga ttaaggcagt aaattaagca gcggttttca gctggcttta
      241 cgctcatgtg cctgacggta tgccaccagg tcttcaatag tcacgagcgc catgttgtgt
      301 ttattggcaa actcaatgca ctctggtgca cgcgccatcg tgccatcgtc attagtcagc
      361 tcacacagca caccagccgg tttgaagcct gccagcgtca ttagatcaat agttgcttca
      421 gtatgaccgc cacgcgtcag tacaccacca gcctgagcgc ggagtgggaa aacgtggccc
      481 gggcgattca gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata
      541 cggtcagcgg cagaaacacc ggtagtcaca ccttcagccg cttcaatggt cacggtaaaa
      601 ccggtgccat aggcgctggt gttattttct accatcattg gcagatcgag ttgtttacgg
      661 cgatcttcag taatgcacag gcaaacaata ccactaccgt ggcgaatggt cagcgccatc
      721 tgctcaacag tcatggtttc tgccgggaag atcatatcac cttcgttttc acggtcttca
      781 tcatcaagca ccattacacc gcgtccttca cgcagcgcag ccagtgcatt ttcaacacgt
      841 tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa acctcactaa
      901 aattatggtt accagaatca gggcagtctt aggagtggcg gcatatagcc aaaataacgt
      961 gagcgggtcc atgcccgaca gaatcgttac tctctcccat ccggactcta accgtcggcc
     1021 ccggaattac accggatctg ctgtcctttg agttcgcacc caaagcgctc gcgggctttc
     1081 aactgagttg atttaccgcc ggtggggaat ttcgccccgc cctgagaata agcggattca
     1141 ctataacgct aatgattagc ggcagcaacg catagcttca cataattctg gtttatgact
     1201 tacccttatc gcactacaat ggcactcaac acctatcagt acagggaaac tacaatgatt
     1261 gacccgaaaa aaattgagca aatcgctcgc caggttcacg aatcaatgcc taaaggaatc
     1321 agggagttcg gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc gcagctgacg
     1381 cgtctcgatc tggtaagccg cgaagaattc gacgttcaga cgcaagtcct gttgcggact
     1441 cgtgaaaaac tggcgttact ggagcagcgc agcagtgaac tggaagcgcg caataattcg
     1501 gtagcagatc ttcaatctcc tccggctatc ccgcctattg ataaagcaga ataaagcaac
     1561 acaaaaacgg gccagacggc ccgtaatatc agtgttctaa ttcaagttct ctggtttgca
     1621 tcatctgttg gcaataccag gcataacgtg cgcaaaacca ggttctatgt tcttcatcca
     1681 tgttaccagt aacggccaga atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac
     1741 tacgcgctag gaaatcgaaa ttatttaaag aattaagact atgatccata agcacatcct
     1801 ccggttatcg catacctttt aaggccatat gctttttctg atttcagtat tccccaatca
     1861 gtgtagtgcc tttaaacata acgtgcgtaa atatattgtc cccgatcaca ctttttagtg
     1921 aaaaataaaa aaccatatta aaagactttc tcttccagaa cctctctaac atatgttatt
     1981 attaacaaag acaaatgtaa gcaaggaaac tatcacaaag taaatattgc gtcactaaat
     2041 ggacattgga gtgacatatg attttattcg ccgactataa caccccttat cttttcgcta
     2101 tttcctttgt actgttgatc ggccttttag aaatttttgc gcttatctgc ggtcacatgc
     2161 tctccggtgc tcttgatgca catcttgacc attatgattc cataaccaca ggtcatatta
     2221 gccaggcact tcattatctc aatatcggaa gattgccagc tctcgtcgtt ctttgcttac
     2281 tggctggttt ttttggtctt attggcatcc tgttgcagca tacctgcatc atggtctggc
     2341 aatcaccact ctcgaacctg tttgtggttc ctgtcagttt actatttacg attattgcgg
     2401 tgcattatac cggtaaaata gttgcgccct ggattccacg cgatcatagc tcagctatta
     2461 cagaagaaga atatattggt agcatggcgt taattaccgg tcatcaagcc acttcaggta
     2521 atccctgcga aggaaaactc accgatcatt ttggccaaat acattattta ctactggagc
     2581 ctgaagaagg aaaaattttc actaaaggag acaaagtgtt aattatttgc cgactttctg
     2641 caacgcgata tttggcggaa aacaatccct agccccaaat attgtaattt attaatcaaa
     2701 aggaaattta ataatggatg atattgttaa ttccgtgccc tcctggatgt ttaccgcgat
     2761 tattgccgta tgcattctgt ttattattgg aattattttc gccaggctct atcgtcgcgc
     2821 ttcggcagag caagcatttg tccgtaccgg cttagggggg caaaaagtgg taatgagcgg
     2881 tggcgcaatc gtgatgccga tctttcatga aataatcccc atcaatatga atactctgaa
     2941 gctggaagtc agccgctcaa ccattgatag cctgattacg aaagatcgta tgcgcgtcga
     3001 tgtagtagtc gctttctttg tgcgggtaaa accttcagta gaagggattg ccaccgctgc
     3061 ccagacgctg gggcaacgca ccctgtcgcc tgaagactta cgtatgttgg ttgaagataa
     3121 atttgtcgat gccctccgtg caacagctgc gcaaatgacc atgcatgagt tacaggatac
     3181 ccgcgagaac ttcgtgcagg gagtacaaaa taccgtagct gaagatctgt cgaaaaacgg
     3241 cctggaactg gaaagcgttt cacttaccaa ctttaaccag acctcgaaag aacatttcaa
     3301 tcctaacaat gcctttgacg ccgaaggttt aaccaaactg actcaggaga cggagcgccg
     3361 tcgccgcgag cgtaacgaag ttgaacagga tgtagaagtt gcggtgcgtg agaaaaaccg
     3421 tgatgcactt tcgcgcaagt tggagattga acaacaagaa gcgtttatga cgcttgagca
     3481 ggagcagcag gttaaaaccc gtaccgctga gcagaatgcg aaaattgcgg cttttgaagc
     3541 tgaacgtcgt cgtgaagcag agcagacgcg aattctggct gaacgacaga ttcaggaaac
     3601 agaaatcgac cgcgaacagg ccgtccgctc aagaaaagtt gaagctgaac gtgaagttcg
     3661 cattaaagag atcgaacagc agcaggtcac cgaaatcgct aaccagacga aatcgatcgc
     3721 tattgccgcc aaatcggaac aacagtccca ggcagaagcg cgtgctaacc ttgcactcgc
     3781 agaagcagta agcgcccaac aaaacgtaga aaccactcgc cagacagccg aagccgatcg
     3841 tgccaagcag gtggcgctga ttgccgcagc acaggatgca gaaaccaaag cggttgaact
     3901 gaccgtgcgg gcgaaagcag aaaaagaagc cgcagagatg caggcggcgg cgatcgttga
     3961 gttagccgaa gctacgcgta aaaaaggcct ggcggaagca gaagcacaac gtgcgctgaa
     4021 cgatgctatc aacgtacttt ctgacgaaca aaccagcctt aaattcaaac tggccttgtt
     4081 gcaggcgcta cctgcggtaa tagagaaatc cgtggaacca atgaagtcca ttgacggtat
     4141 caagattatt caggtcgatg gtctgaatcg tggtggtact gcgggggatg caaacacggg
     4201 taatgtgggg ggcggaaacc tggcggaaca agcattatca gccgcactct cttaccgcac
     4261 acaggcacca ctgattgact ccttgctcaa tgaaattggc gtttcaggcg gctcactggc
     4321 tgcattgact tcactcttaa ccccaacaac tcccgtcgcc gaaaacgtag aataaatttt
     4381 cagctatgaa catccctggc tcaaaaagcc ggggattttt tatatctgcg ttccgctaaa
     4441 aggtgcaaat gatcaggctg ttgcagcgtt ttgcgtgatt gctcggggaa ggcaaaactg
     4501 cttcagggaa agcgttgcgc ggggtccggc gctcatcaac aatcggggag taagcaaagg
     4561 gctgaaacgg gaaagcccct cccgaggaag gggccttaca gaaggaaagg gttatgatga
     4621 agctcgtcat catactgatt gtgttgttac tcgtaagttt cgcagcttat taacagccaa
     4681 tcagaggggg gagaaatcct ccctcaccct tgttccttta ctttaggttg aaaaaaaatc
     4741 agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg attttttata
     4801 tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc gtgaccgctc
     4861 ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc atcaacaatc
     4921 ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc cttatagaag
     4981 gaaagggtta tgatgaagct cgtcatcata ctggttgtgt tgttactgtt aagtttcccg
     5041 acttactaac aactcatcag aggggggaga aatcctccct tacccttgtt cctttactct
     5101 aggttgaaaa gacaacagcg tcaataggcc tgccatgtac gaagcgagat ctgtgaaccg
     5161 ctttccggtt agcctttttt atcctgttgg atcttcttga taatgttagt ggtcgagcaa
     5221 ccgtcttcaa agttgagcac caacacttcg ccgccgttgg cccagacttc tttactcccg
     5281 gcgatctctt ccggtttgta gtcaccgcct ttcaccagca ggtctggcag gatcccggcg
     5341 atcagacgct gcggcgtgtc ttcttcaaac gacactaccc agtcgaccgc ttccagtgcg
     5401 cccagcacaa tcatacgctg ttcgagtggg tttaccgggc gggaatcccc tttcagccgt
     5461 ttggtggagg catcgctgtt gacggcaaca atcaagcggt cacccagctt gcgggcattt
     5521 gccagataag agacgtgccc ggcatgtagg atatcgaaga caccgttggt cattaccact
     5581 ttttcaccac gtttacgcgc tgccactaca gccagcttca gttcctcttc ggtcatcacg
     5641 ccaaagccgg tatctgcgcg tccacgtaca gcgttttcca gctcgatcgg cgaaaccgtg
     5701 gacgtcccca gtttgccgac caccacacca gccgccgcat tggcaaagaa gcaggcttct
     5761 tccagcgaat tacccgctgc cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca
     5821 ccggtaacgt catacacttc ctgcgcctga gttggcatat gcagcggcgc tttacccggt
     5881 tgcagcagcg acataccctg ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg
     5941 gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg tcttacattt accgacaaca
     6001 gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg
     6061 gtaccttttg gatcaatcag caccggaaca cccgctttac gcgccagttg gatcatctgc
     6121 tgtacgcttg ccagcgcacc tttggcgtag tcagaaagca ccagcgcgcc aatcgaactc
     6181 agcgcctgat taatccgctc gtgcagcggc tgcggatcaa caccttcgaa accttcttca
     6241 aaatccagac ggatcagctg ttggttgcgg gaaagtaccc gtaatttggt aatggtcgga
     6301 tgcgtcggta cagaaacgaa gtcgcatttg acgttgacgt cggccagaga tttactcagc
     6361 gcgcgcgctg catcgtcaat gcccgtcaac ccgaccaggc gtgcattagc accgagagaa
     6421 gcgatattca tcgccacgtt agccgcgccg cccggacgtt cttcgatggt attcacttta
     6481 accacgggca ccggcgcttc cggcgagata cgactggagg ggccgtacca gtaacgatcc
     6541 agcatcacat caccaaccac catcactcct gcacgttcaa actctggcag cgttactttc
     6601 attcctgtct cctgagagat tcaaaatttg cgcgcgataa taccatactt cattcttcca
     6661 ccagccactt ctgccagctt gcccgcacca gttcacgctc tgcggtgaag caatcctccg
     6721 gcacatggcc cggcaattcc tgtaatgcca gatgatgaag ttcatcgcga agcgtagtgt
     6781 aagcacgggt cagcgccatc gcttcctgct cttccataat gtcgttttgc gccagtagtt
     6841 ccagaatgcg cacgttgtct gaccagcgcg ttaacttcgg tttttcatga gcgtagcgca
     6901 acaccagata ttgggtaata aattcgatat cggtaattcc cccttcatca gctttgatat
     6961 caaagcgatc gcgatgttta ttgccgagat gagcgcgcat tttctcgcgc atttcccgca
     7021 cttccgtttg cagagtttta ccttcacgcg gcagcgtcat aatctcgcga cgcactgcgt
     7081 caaagtgcgc ggtgagctgc ggatcgccgt acactacacg cgcacgcacc agcgcctgat
     7141 gctcccacgt ccaggcctcg tttttctgat aatcggcaaa tgcttctgtg gaggtaacca
     7201 gcattcccgc cgccccggac gggcgcagcc gcgcatccac ttcgtacaaa atgccggaag
     7261 aggttcgcgt gctgaacagg tgcataatgc gttgcgccag acgcagataa aactgtcgtc
     7321 cgtcgatttc ccgctcgccg tcagtcatcg catccattgg gcaatcatgg aggaagatca
     7381 ggtcaagatc ggaactgtag cctaactccc agccgcccag cttgccgtag ccgaccactg
     7441 caaaaccacg cccttcgcgt tcgttcaggt gattcggctt accgtagcgg gcaaccattt
     7501 gaacccacgc ctgctgaacg acggcatcta tcatggcttc cgccagccag gttaagtgat
     7561 cgctcacttt catcaccggt agcgtaccgg cgatatccgc tgcggcgatg cgtaacagct
     7621 gcgcctgttt gaactgacgc agcgcctcaa gctgttgctc ttcgtcatct tccggcacgc
     7681 gcagcaaata ctggcgcaac tcatcgcggt aggcatcggt cgccgtcggc tggtaaaggg
     7741 tgtttggatc gagcaattca tccagcaata atggataacg cgccagctgg ctggcaatca
     7801 tcggcgacgc tgcacacaga gaaatcaaat gtttaagcgc cgcggggaat tcactgagca
     7861 attctaaata ggtggtgcgg gtaacaatcc ccgccagcaa ggcggtaatg cgcaacagcg
     7921 taacggcagc gtcttcacgc gcacagacat cacttagcag atgcggcatc agatggtcga
     7981 gcacctgacg cccgcgtggc ccgattgtgc gcttatccag ctctttgcgg aaatcggcaa
     8041 tcagcgtcag cacctgtttg cgatcgtcct cgctaagatg cgccagcact ggtgtagtgt
     8101 catcttcctg caacgcatcc tgccacagct cgcgccactg ttccgacagc gactcttcct
     8161 gagtttcact ttcgtcgtcg ccaatcaatt cattaaacac ccggcgcaca ttggtcatat
     8221 gtgcggtcag cgccccggtc aattgcggcc agtcagcaaa gtccatcgcc cacgccagcc
     8281 gcgcacggtt aagctcatca gaaggaagcg tctgggtttg ttcgtcgtta atgctttgca
     8341 gcaggttttc cagacgccgc aggaacagat acgccactcg caattgttca gcatcgtttt
     8401 cagaaagcag atgcagcgcg gcaatgacgc tgagcgttgg cagtaaagag cgcgactgca
     8461 gcgacggttc gcgcccgccg cgaatgagct ggaacacctg aacgataaat tcaatttcgc
     8521 gaatgccgcc tgcgccgagt ttgatattgt cggtcaaacc acgtcgacgc acttcacggg
     8581 caatcattcc tttcatgttg cgcagcgact gaatcacgct gaaatcgatg taacgacgga
     8641 aaacaaacgg gcgcagcatc gcacgcaact cgttagcata gacgccttcg ctatcgccca
     8701 taatccgcgc cttgaccatc gcgtaacgct cccagtcgcg cccctgctcc tggtaataat
     8761 cttccaacgc ggcaaagctc agcaccagcg ggccactttc gccaaacgga cgcagccgca
     8821 tatccacgcg atagacgaag ccatcctgcg ttggttgatc cagcacttta atcagccgct
     8881 gccccatgcg ggtaaaaaac tgcgcgttat ccagttcccg gcgtccaccc tgcgtacaac
     8941 catgttccgg ccaggcaaaa atcagatcga tatcagagga gaaattcagt tccccgccgc
     9001 ccagcttacc catgcctaaa atcagcagcg gttgcgcttc gccctgcgca ttgcacggcg
     9061 ttccccactc gcggcagcag gcgtcatata gccagtcacg cgccgcaaca atcagcgtct
     9121 ccgccagata gctgagctgc tgcaatatgc tctcttcagt aaccagtgcc agcgtttgcg
     9181 cccaggcgat acgtaccatg atgcgccgac ggaacagccg cagctcgcgc attaacccgg
     9241 cttcgtcact cacattaatg agcgcctcct gcaaccatgc aacgtaatgc tgccattcgt
     9301 cggcctgcgg cggttggctt tccagttccg tcagccactc tggatgcgca cttatgctgt
     9361 cctgcacaaa atcactgaaa gtaagtactg acttcgcctg tgcactaagt gattcctcgg
     9421 ctaaaggctc tggcagccgc tcaacaatgg tctgccagta ctgctgtaac ggtgaagaga
     9481 gcggcttcat aagcgatttt atccttgcct gattaacgtt ttccgctgtg caaccagaac
     9541 ggttcctgat tgtttgcctc attacggaaa tgttcaattt cgatgcgttg cccggtcgca
     9601 atagcgtgat gcagcccctg ccagttctcc agccaggctt gcgcgacgac aggatcatag
     9661 taacccgcca gcaacagtat tgagtcaata tcacgcgtca ggcgtggcaa ctggtcacgg
     9721 tagcgatcgc ctaacggctg gcagaaaacg cttttcagtt cagcggcatg gcgggaaaga
     9781 tggatatcgg caaagcgttt gaaggagtcg ctgattttgc cctgcgcttt ggcatctaaa
     9841 aatggctgcc atgctttgct taccaaccat tcggtcaacg ccagcttcgc cattgccgtt
     9901 tcggtagagt agaccgccgt cacggcagaa accgcagaag caatggtcgc ttcgcattgg
     9961 gtcagcaaat cacgtaagtg agtgctcgct ttacgcggca caataccacc gaacagcatc
    10021 agcgtatgac ggaccaagct aatggctgcc agcacctgtt ctttcgccgc atcgttgccg
    10081 cgtacccaca gttcttcatg atactgccat tgcgctaacg ccagctcgag cgccgcttcc
    10141 agcccctgtt ccacatcggc ttttgccgca acatgcaaaa tggtggtcgg tttgatttca
    10201 cgcgccggat tgccctgcgc cagatgatag ccacgcgccg ctttgctcag gctgccctgg
    10261 cgtaatccgg tttgcgatac cagttggttc gccagtttca gcaccgcgcg cgtgtcgccg
    10321 ctaagcagct ccagttccag ctcacagata ggttcagcaa attcccccgc tttcacctca
    10381 ccctgatcga gagcgatttc aattcggctg ccatcgaccg ccaccagcca tttttcgcga
    10441 taaaaatcgg tgctgaacag cggttgtacg cgggaagcga gatcggcggg caattcgccg
    10501 ttcggccaga cttccgtcgg taactgcgcc aggtcgagcg tcggttcgct caacgccacg
    10561 ttatattccg ggcgctggtg taagccgcct gtcactcttc ctgcaacttt catggtcatc
    10621 tcatagcgac cgttttcgcc acgaatacgt aagcccatat cgtgcccacg cagccagtta
    10681 tccggcgttt cgtagtaaat attcagtaac tgcacggggt catggtgctc gccgcccagc
    10741 gtattgagat ggtcacgcaa cgcctcaacg gcgctgtgat taacaataaa ctttaattcg
    10801 atttcctgag ccatggcctt tacttatcgg ttatgtcaca taagtgacga tgaacggcga
    10861 acttaatgcg atcttttttg tcagtagata gtattttgcg ccaaattgcc atgcaacggg
    10921 caatttgacg ggcgtaaaag tttgaagcag tggcaagacc tgacacagag gattccgatt
    10981 gatgtcgaat gctttgcgta gcgacactga tgccactact atcgttccac tttccatgac
    11041 aataacgaca gcctgatgcc aaaattacgc ctgatcggat taactttact cgcacttagc
    11101 gcgactgccg tctcacacgc tgaagaaacg cgctatgttt ctgacgaact gaatacctgg
    11161 gtccgtagcg gtccgggaga tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa
    11221 gtgaccttat tacaaactga cgccaacacc aattatgccc aggtgaaaga cagctctggc
    11281 cgtaccgcct ggatcccgtt gaaacaactt agcactgagc caagcctgcg ctcccgtgtg
    11341 ccagatctgg aaaatcaggt caaaaccctg accgataaac tcaccaatat cgataacacc
    11401 tggaatcagc gcaccgcaga aatgcagcaa aaagtggcgc agagcgacag cgtgatcaac
    11461 gggttaaaag aagaaaatca gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc
    11521 gatgccgcca gcgtgcagct ggatgacaaa cagcgcacca tcatcatgca atggtttatg
    11581 tatggcggcg gcgtgctggg gcttggcttg ctgctcggtc tggtactgcc gcacctgatc
    11641 ccaagccgca aacgcaaaga tcgctggatg aactaaatcg ccttcttcgt tgcactgtct
    11701 tattattagg aattgtgtat ttgacgaagg gatgatggcg tgaagattta tctggtcggt
    11761 ggtgctgttc gggatgcatt gttagggcta ccggtcaaag acagagattg ggtagtggtc
    11821 ggcagtacgc cacaggagat gctcgacgcg ggctaccagc aggtaggccg cgattttcct
    11881 gtgtttctgc atccgcaaac gcatgaagag tatgcgctgg cacgtaccga acggaaatcc
    11941 ggctccggtt acaccggttt tacctgctat gccgcaccgg atgtcacgct ggaagatgat
    12001 cttaagcgtc gcgatctgac cattaatgcg ctggcccagg acgataacgg cgagattatc
    12061 gacccgtaca acggtctggg cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct
    12121 tttggcgaag atccgttacg cgtactgcgc gtggcgcgtt ttgctgcgcg ttatgcccac
    12181 ctcggttttc gtattgccga tgaaactctg acgctgatgc gcgagatgac ccatgcgggt
    12241 gaactggaac acctgacgcc tgaacgggta tggaaagaga cagaaaacgc cctcaccacc
    12301 cgcaatccac aggtgttctt ccaggtactg cgcgattgcg gcgcactgcg cgttttattc
    12361 ccggaaattg acgccctgtt tggtgttccg gcccctgcca ggtggcatcc ggaaatcgac
    12421 acgggtattc ataccttaat gacgctctca atggcggcga tgctgagtcc gcaggtcgat
    12481 gtccgtttca caactttatg ccacgatctc ggtaaaggac tgacgccgcc agaactctgg
    12541 ccgcgtcatc atggtcatgg cccggcgggt gttaagttag tggaacaact atgccagcgt
    12601 ctgcgcgtgc caaatgaaat tcgcgattta gccagactgg tggctgagtt tcacgatctc
    12661 atccacacct tcccaatgct gaacccaaaa accatcgtca aattatttga ttccatcgat
    12721 gcctggcgta aaccgcagcg tgtcgagcaa ctggcgctga ccagcgaggc tgacgtgcgc
    12781 ggcagaaccg gttttgaatc agcggactac ccgcaaggcc gctggttgcg cgaagcctgg
    12841 gaagtggcgc agtcagtgcc gacaaaagcc gtcgttgaag cgggatttaa aggtgtggag
    12901 attcgcgagg agctgacccg acggcggatt gcggcggtag ccagctggaa ggaacaacgt
    12961 tgcccaaagc ctgagtgagt attgggcatt ttgccggatg cggcgtaaac gccttatccg
    13021 gctacatgtc aacgacagtt gtaggcctga taagacgcgc aagcgtcgca tcaagcgttg
    13081 attgccggac gcggcgtgaa cgccttattc ggcctacgca atcgtggaca acgccaggca
    13141 tccgacacta ttcctcaatt aaaagaacac gacatacacc gcagccgcca caataaagcg
    13201 ataaatggcg aacgggataa acgaaatgcg cttaatcaat tgcaggaagg ttttaatcgc
    13261 tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga tatcgccgct
    13321 tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca tcatcggcac
    13381 cgccagcagg aacgaaaact cggaagcagc gtaacggctc acccccatca gcatcccacc
    13441 tgaaatggtc gccccggaac gggagaaacc cggccacagc gccagacact ggaaacaacc
    13501 aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg gctctttcgg
    13561 cttcaggcat tcggcggcaa tcagcaacaa accgccaacg accagcgcat acatcacatt
    13621 tatcgggtta aacaatgact taatcgtgtc gtggaacaac agccccagta ccaccgccgg
    13681 aatcatcccc agcaaaatgt ggatcagcgt taaacgacct ttgctttctc cttcgtgctg
    13741 caacgggcgg ccaaagtgga tgccaatcag gccaaacaga cgccgccaga acatcactac
    13801 taccgccaga attgatccta actggatcac aacttcaaag gttttcgccg tgtcgccctc
    13861 aaaccccaac aagtgaccga caataatcat atggcccgtg ctggataccg gcagaaattc
    13921 tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca tatcgctcat
    13981 caataaaccc ctaaattata aaaatgtaac gcttgtccga actactgcag tatgaccagg
    14041 ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat tattgccacg
    14101 ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc tgagtttgat
    14161 acgcacccac ggcgagttga agcgtgctaa cagcagctct gccacctctt ccgccacgcg
    14221 ctccaccagc gcaaaacgtg ccccctcgac gtggctgacc accgtttctg caatgtcagc
    14281 gtagctgagg caatccgcca cattatcgct ttttgccgct ttacggttat cccacgccat
    14341 ttcgatatcg aacactaatt tctgttcgat ggtctgttcc cagtcgtaaa caccaatagt
    14401 ggtgattacc gaaagttgct ctataaatac aatatccatc acgacctgct cgctttttgg
    14461 ctaacccgga taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgtttaaca
    14521 cgaattttca aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg
    14581 tacctctgcg gctccatttc cagtgccatt ctggtttgcc gcctgtgtgg gctgcccgat
    14641 ccgcgaacca gcggctccgg taatcctggc gcaactaacg tgttacgtat aggtggcaaa
    14701 ggagcagccg tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc
    14761 gcgtatgaat taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt
    14821 ggacacatct ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt
    14881 ggtgccatcg cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg
    14941 accgtgctat tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg
    15001 ttttatgtct ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg
    15061 atcctgctgc gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc
    15121 tggacgaaat tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat
    15181 gacctgatag ccttcatcgg gtttgcccag ccgttgctgg caccatgccg ccagaaattc
    15241 cacgcagaca cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt
    15301 ggcgctttgt gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa
    15361 cacatcccac tcggaacgca gcataatccc tttgccctcc agcgcccatt gcagcacaat
    15421 ctcgccgcta ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt
    15481 ccccaactcc catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg
    15541 acttaattcc tgtagggatt gtggctgcgg atatttttgc agatactcag gtgctgcgca
    15601 taatattctt ttatttttcg
//
LOCUS       DAZKFZ010000100        15239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000100 DAZKFZ010000000
VERSION     DAZKFZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..351)
                     /locus_tag="ACHOQW_002819"
     CDS             complement(<1..351)
                     /locus_tag="ACHOQW_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573870.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSL"
     gene            complement(582..1556)
                     /gene="yajO"
                     /locus_tag="ACHOQW_002820"
     CDS             complement(582..1556)
                     /gene="yajO"
                     /locus_tag="ACHOQW_002820"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP9573871.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDMVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(1611..3473)
                     /gene="dxs"
                     /locus_tag="ACHOQW_002821"
     CDS             complement(1611..3473)
                     /gene="dxs"
                     /locus_tag="ACHOQW_002821"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9573872.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(3498..4397)
                     /gene="ispA"
                     /locus_tag="ACHOQW_002822"
     CDS             complement(3498..4397)
                     /gene="ispA"
                     /locus_tag="ACHOQW_002822"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP9573873.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(4397..4639)
                     /gene="xseB"
                     /locus_tag="ACHOQW_002823"
     CDS             complement(4397..4639)
                     /gene="xseB"
                     /locus_tag="ACHOQW_002823"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9573874.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4845..6293
                     /gene="thiI"
                     /locus_tag="ACHOQW_002824"
     CDS             4845..6293
                     /gene="thiI"
                     /locus_tag="ACHOQW_002824"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9573875.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(6347..6937)
                     /gene="yajL"
                     /locus_tag="ACHOQW_002825"
     CDS             complement(6347..6937)
                     /gene="yajL"
                     /locus_tag="ACHOQW_002825"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP9573876.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6900..7811)
                     /gene="panE"
                     /locus_tag="ACHOQW_002826"
     CDS             complement(6900..7811)
                     /gene="panE"
                     /locus_tag="ACHOQW_002826"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9573877.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            7979..8470
                     /gene="yajQ"
                     /locus_tag="ACHOQW_002827"
     CDS             7979..8470
                     /gene="yajQ"
                     /locus_tag="ACHOQW_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP9573878.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(8598..9962)
                     /locus_tag="ACHOQW_002828"
     CDS             complement(8598..9962)
                     /locus_tag="ACHOQW_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9573879.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(10111..11001)
                     /gene="cyoE"
                     /locus_tag="ACHOQW_002829"
     CDS             complement(10111..11001)
                     /gene="cyoE"
                     /locus_tag="ACHOQW_002829"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP9573880.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11013..11342)
                     /locus_tag="ACHOQW_002830"
     CDS             complement(11013..11342)
                     /locus_tag="ACHOQW_002830"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP9573881.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(11342..11956)
                     /locus_tag="ACHOQW_002831"
     CDS             complement(11342..11956)
                     /locus_tag="ACHOQW_002831"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP9573882.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(11946..13937)
                     /gene="cyoB"
                     /locus_tag="ACHOQW_002832"
     CDS             complement(11946..13937)
                     /gene="cyoB"
                     /locus_tag="ACHOQW_002832"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP9573883.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(13959..14906)
                     /gene="cyoA"
                     /locus_tag="ACHOQW_002833"
     CDS             complement(13959..14906)
                     /gene="cyoA"
                     /locus_tag="ACHOQW_002833"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP9573884.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         3827 a         3971 c         3885 g         3556 t
ORIGIN      
        1 caatgaggat gcaattttat tccagaaaca acaaagcgtg gtctgttttt gtttgggcga
       61 gaggcgatca aaatccagct ttaaatatga gtctaaatct gacgttgcat ctatattaaa
      121 gttgggatct ggagttaacg ctgaattata atttagaact gacaatgtat tgatgtattt
      181 atgaatcgat ttaaatgcct tttgtttatc actgtccaga aaaaaatcac gaattttgtt
      241 ccacagcctt tcaataagat tttgatttaa atcttgcttc attgagtttc ttaaatctgc
      301 aaccggtaca tcagtaaaca ggatcggttg tttggtgatg gttttcatca tgattgtaat
      361 cctcttacgt tgactaaata agacagtttt aggggatatt acctggagaa actatcaaaa
      421 atcgctcaaa gggatgaaga cgatgaatag ctgcaaactc attcacgaag aatttctctt
      481 gcagaggtgg attatggtta cagaaggtga tgggaggtac gttgtcccca tcatcgcaga
      541 cgatggggaa ttcagcagat aaaatgggtg aaaagcgaaa tttacttaaa tcctacgacc
      601 ggatgcggtt tatacggtgt ttccagttca gcaatctgtt ccggcttcaa agtgatatcc
      661 accgcgttca gcagctcatc aagctgttct tcccgcgaag ttccgataat cggtgcggca
      721 atgcccggtt tactcaacaa ccaggccagc gcaacttgtg ctcgtgttgc accgagttct
      781 tcactgacgc ccgttaaccg ctctgcgatc tgcgcgtcat tttcatcgct ttctttatag
      841 agatttttcc ccacctcatc agacaccagt cgtgcggtag tttctcccca cggacgcgtc
      901 agtcgccccc gcgccagcgg actccacgga atgaccgcca cgccttcctg ataacacagc
      961 ggcagcatct cgcgctcttc ttcgcgataa atcagattgt agtgatcctg catactgaca
     1021 aactgcgccc agccgtgctg tttttgtagt tccagtgcct gagcaaactg cgaagcgtgc
     1081 atggatgacg cgccgatata acgcgctttc ccggctttta ccatgtcgtt gagggcttcc
     1141 agcgtctctt cgatcggcgt gttgtaatcc cagcgatgaa tttgcaggat atcgacataa
     1201 tccatgccga gacgtcgcag gctgtcgtcg atagagcgca aaatttgtgc acgggataat
     1261 ccttccggta aatcaccaac gcgatggaac actttggtgg caacgaccac gtcttcacga
     1321 cgggagaaat cccgcagtgc gcgaccgacg atctcttcgc tgctgccatc ggaatagcta
     1381 ttggcggtat caaagaaatt tatgccgcct tccagtgcgc gtttaatgat gggacggctg
     1441 ctctcttccg gcagtgtcca tgcgtgatta ccacgatctg gctcgccaaa ggtcatacag
     1501 ccgaggcaaa gtcgggaaac gcgaaggtcg gtttttccta aggggttgta ttgcatgctg
     1561 ccactcctgc catgaataat ttcttaagca tagcaggagt ggagtaggga ttatgccagc
     1621 caggccttga ttttggcttc cataccggcg gcatcgaggc cgagttcggc gcgcatttct
     1681 tcctgagttc cttgcggaat aaagaagtcc ggcaggccaa tgttcagcac gggtactggt
     1741 ttacgatggg ccatcagcac ttcgttcact ccgctgcctg caccgcccat aatggcgttt
     1801 tcttctacgg taaccagtgc ttcatgactg gcggccattt ccagaattaa cgtttcatca
     1861 agcggtttca caaaacgcat atcgaccagc gtggcgttca gcgattcggc gactttcgcc
     1921 gcttctggca tcagcgtacc aaagttaagg atcgccagtt tctcgccacg acgcttcaca
     1981 atgcctttgc caattggtag tttttccagc ggcgtcagtt ccacgccgac cgcgttgcca
     2041 cgcgggtagc gcaccgctga cgggccatcg ttatagtgat agccggtata gagcatctgg
     2101 cgacattcgt tttcatcgct cggggtcata atgaccattt ccggtatgca gcgcaggtaa
     2161 gagagatcaa aagcgccctg gtgggtttga ccgtcagcac caacaatgcc cgcgcggtcg
     2221 atggcgaaca ggaccgggag tttttgaatt gccacgtcat gcagcacctg atcataggcg
     2281 cgttgcagga aggtggagta aattgccacg atcggtttgt agccaccaat cgccagaccg
     2341 gcggcaaagg tcaccgcgtg ttgctcggca atcgctacat caaaatagcg atccgggaat
     2401 ttacgtgaaa actcgaccat gccggaacct tcacgcatcg ccggagtaat cgccatcagt
     2461 ttgttatctt tcgctgccgt ttcgcacaac cagtcgccaa agatttttga atagcttggt
     2521 aaaccaccgc tacttttcgg caaacaaccg ctggagggat caaatttagg cacggcgtgg
     2581 aaggtgattg ggtctttttc tgccggttca taaccacgac cttttttggt catgatatgc
     2641 aggaactgcg ggcctttcag gtcgcgcatg ttcttcagcg tggtgataag ccccagcaca
     2701 tcgtgaccgt caaccgggcc gatgtagtta aagcccagct cttcaaacaa cgtgccaggc
     2761 accaccatgc ctttaatatg ttcttcggta cgtttgagca gctctttaat tggcggaacg
     2821 ccagagaaaa cttttttccc gccttcgcgc agtgaagagt aaagcttacc ggaaagcagc
     2881 tgcgccagat ggttgttaag cgcgccaaca ttttcggaaa tcgacatttc attgtcgttg
     2941 aggaccacca gcatatcagg acgaatatcg cccgcgtgat tcatcgcttc aaacgccatg
     3001 cctgcggtaa tcgcgccgtc gccaatcaca cagacggtgc ggcgattttt gccttctttc
     3061 tcggcagcaa ccgcaatacc aattccggca ctgatggagg ttgatgaatg cccgacgctt
     3121 aatacgtcat attcgctttc gccgcgccac gggaacgggt gtagaccgcc tttctgacgg
     3181 atggtgccga ttttgtcgcg gcgtccggtc aaaattttat gcggataagc ctgatgcccc
     3241 acatcccaaa tcaattggtc aaacggggtg ttatagacat agtgcagcgc cacggtcagt
     3301 tcgaccgtgc ccagcccgga ggcgaagtgc ccgctggaac ggctcacgct gtcgagtaaa
     3361 tagcggcgca gttcgtcgca gagtttcggt aaactctctt ttggcaacag tcgtagctcc
     3421 tgggtggagt cgaccagtgc cagggtcggg tatttggcaa tatcaaaact catcaggggc
     3481 ctattaacac ttattgttta tttattacgc tggatgatgt agtccgctag cgcttccagt
     3541 gccgaagtat cgatcgactg ttcagccagt tgtttcagcg actggcgggc atcgtcgatc
     3601 agatcctggg ctttcttccg ggcttgctca agacccagga gtgcagggta ggtacttttg
     3661 ccaagctgct ggtcggcacc ctgacgtttt cccaacgttg cagtatctcc caccacatcc
     3721 aggatgtcat cctgaacctg gaaggcaagg ccgatgctct ctgcgtactt gtcgagtact
     3781 ggtagagcac gacgtccttt atctccggcg cttaatgcac caaggcgaac ggcggcgcga
     3841 attaatgcgc ctgttttatg acgatgaata cgctcaagcg cgtccagagg tacgtgtttg
     3901 ccttccgcgt ctaaatccaa cgcctgacca ccacacattc cggcaatacc gctggcgctc
     3961 gccagttcag aaatcatcga aattctgtcg cgatccgaca cttctggcat atcagcatcg
     4021 cttaaaatcg agaacgccag cgtttgtaaa gcgtcgccag cgagaatcgc gttcgcttcg
     4081 ccaaacttca catggcaggt cggcaagccg cgacgcagat cgtcatcatc catcgccggt
     4141 aaatcatcat gaattaatga gtaagcgtgg atacactcta cggcggcagc gggtgcgtcc
     4201 agcgtgtttg tgctaacgcc gaacatatga ccggtggcat aaaccaggaa aggtcgcagg
     4261 cgcttaccac ctaataatgc gccatactgc atggtttcga ccacgggagt gttctgaaag
     4321 ggcagtgggg cgataaaacg gctcagcgcc tggttggcct gcttaacgca ggcttcgagt
     4381 tgctgcggaa agtccattac tcattgtccg gtgtaaaagg ggttagagag gcgtcttcgt
     4441 tgtcagacag cagaatttgt acgcgctgtt cggcttgttg taatttggcc tgcccctgac
     4501 gtgccagctg cacgccgcgt tcgaactcgt tcagcgcctc ttccagcggc aggtcgccac
     4561 tttccagacg ggttacaatc tgttccagct cgctcagcgc cttttcaaag ctggcgggcg
     4621 cctcattttt cttcggcata atgaatgtct gactctcaat atttttcgcc ccgtcatggt
     4681 aacggactca gggcaaatag caaataacgc gcaatggtaa ggtgatgtgc gcagcaaagc
     4741 gatgttagtg gtatacttcc gcgcctggat gcagccgcag gtgtgggctg ctgtattttt
     4801 ccctatacaa gtcgcttaag gcttgccaac gaaccattgc cgccatgaag tttatcatta
     4861 aattgttccc ggaaatcacc atcaaaagcc aatctgtgcg cttgcgcttt ataaaaatcc
     4921 ttaccgggaa cattcgtaac gttttaaagc actatgatga gacgctcgct gtcgtccgcc
     4981 actgggataa catcgaagtt cgcgcgaaag atgaaaacca gcgtctgact attcgcgacg
     5041 ccctgacccg tattcctggt atccaccata ttctcgaagt cgaagacgtg ccgtttaccg
     5101 acatgcacga tattttcgag aaagcgttgg ttcagtatcg cgatcagctg gatggcaaaa
     5161 ccttctgcgt acgcgtgaag cgccgtggca aacatgattt tagctcgatt gatgtggaac
     5221 gttacgtcgg cggcggttta aatcagcata ttgaatccgc gcgcgtgaag ctgaccaatc
     5281 cggatgtgac tgtccatctg gaagtggaag acgatcgtct cctgctgatt aaaggccgct
     5341 acgaaggtat tggcggtttc ccgatcggca cccaggaaga tgtgctgtcg ctcatttccg
     5401 gtggtttcga ctccggtgtt tccagttata tgttgatgcg tcgcggctgc cgcgtgcatt
     5461 actgcttctt taacctcggc ggcgcagcgc atgaaattgg cgtgcgtcag gtggcacatt
     5521 atctgtggaa ccgctttggc agctcccacc gcgtgcgttt tgtcgctatt aatttcgagc
     5581 cggtagtcgg ggaaattctc gagaaaatcg acgacggtca gatgggcgtt atcctcaaac
     5641 gtatgatggt gcgtgccgcg tctaaagtgg ctgaacgtta cggcgtacag gcgctggtca
     5701 ccggcgaagc gctcggccag gtgtccagtc agacgctgac caacctgcgc ctgattgata
     5761 acgtctctga tacgctgatc ctgcgtccgc tgatctctta cgacaaagag cacatcatca
     5821 acctggctcg ccagattggc accgaagact ttgcccgtac tatgccggaa tactgcggtg
     5881 tgatctccaa aagcccgacg gtgaaagcgg ttaagtcgaa gattgaagcg gaagaagaga
     5941 aattcgactt cagcattctc gataaagtgg ttgaagaagc gaataacgtt gatatccgcg
     6001 aaatcgccca gcagaccggg caggaagtgg tggaagtgga aaccgtcaat gacttcggcc
     6061 cgaacgacgt gattctcgat atccgttctg tcgatgaaca ggaagataag ccactgaaag
     6121 tcgaagggat tgatgtggtt tcactgccgt tctacaagtt gagcaccaaa tttggcgatc
     6181 tcgaccagaa cagaacctgg ctactgtggt gtgagcgcgg ggtaatgagc cgcctgcagg
     6241 cgctctatct acgcgaacag ggctttaaga atgtgaaggt gtatcgtccg taatttgtgg
     6301 tttttacgtc gcatctggtc agatgcgacg tttgccgcat ccgacactac tcgtaataat
     6361 tataaatccc tgccgccatc accagttgtg atgccacttc atgggctttc tcgcgcccaa
     6421 ccagcaggtc gataattttc agaccaaagt cgatagctgt acccggcccc tggctggtca
     6481 gcaattttac ccgtgcatcc cagacgacgc gcttgtcctg ccattgttca gcgggaattt
     6541 tgtctttcag cgtcgggaag ccggtcatat tgccaatcgg aaagatatcg tgcggcacca
     6601 gcacggtggc tggcgcggcg caaatagccg cgacgatacg cccggaacgg tggaactgtt
     6661 taacggtttc aaccagcagg gtactatcgc gaaaacactc cgcgccttta atgccaccag
     6721 gcagcacgat cacgtcatat tcgccatcag ccacttcgac cagcggcgca tccgccagca
     6781 gcttcacgcc gcgcgagcag gtaatcgcca ggttgccatc gctggctacg ctggcagtgg
     6841 tgactttgat accgccgcga atcaagaggt cgatcgtggt gacggcttca gtctcttcac
     6901 taccaggggc gaggcaaacc agtgccgatg cgctcatatt cactctcctt tctttttacc
     6961 atttcaaaca ggcgggtgtt ttccggtacg gcaatcccat gcgcgcgggc gcggcgtaag
     7021 agaaaaccat tgatatagtc gatttcggtg tggcgcagcg tgcggatatc ctgcaacatc
     7081 gacgagatat tttccgctgt ggcatcaatc acctgcatca cgtaatcacg taaatcttcc
     7141 gctgaagtat gatgcccttc gcgttcgatc actgccgcga cttcttcgca tatttgcata
     7201 atttcttgcg gatgatgacg taattcaccg ttcgggcaat tccagatggc agtcagtgga
     7261 ttaatcacac agttgactgc cagcttgcgc cacagctcgg cgcgaatatt gttatgccag
     7321 gcaacgtcag gcaacacggt ttgcaatata tccgccagat aactgtaatc gccgtcctgt
     7381 tgccgtgctg gaccaatatg cgtgataccg tttgccacat gaataatgac attgccgtcg
     7441 cggcaggcgg catgggtggt ggtgcccatc agtaatggct gctgaatgtt ttgcaattct
     7501 tcgatggtgc ccatgccgtt gtgaattaac agtattggcg tagttacagg cagtgtggac
     7561 gcgaggcttt tgacggcatc ggaaacctgc catgctttca gcgtcaccag gagcagatcg
     7621 ctggtggcaa gaaaatcggg atcgttggcg gtcagcgatt cgttaaatat cgaaccatca
     7681 gtctcaacca gattcacact acaataaggt tgcggtacgc gcagccagcc ctgaacttcg
     7741 tgaccctgtt tacaaagtgc tgtaagccat aattgcccta aggcaccgca tcccaatacg
     7801 gtaattttca ttgttcctcc tcacccgcaa ccactccggg tgttcaataa ggctatccct
     7861 taattgtgca tgctgttgcg actatgcaca attaagggat acgtcctggt gcaggactgt
     7921 tggttattta actttgcagg tattatgctt cgcatcaaaa atgaagggag aggaaaagat
     7981 gccatctttc gatattgtct ctgaagttga tcttcaggaa gcacgtaacg cggtcgataa
     8041 cgcgagccgc gaagtggagt cccgttttga cttccgtaac gttgaagcct catttgagct
     8101 gaacgacgcc agcaaaacca tcaaagtgtt gagcgagtcc gacttccagg tcaatcagtt
     8161 gctggatatt ctgcgtgcca agctgctgaa gcgcggcatt gaaggcagtt cactggatgt
     8221 accggaaaat atcgttcata gcggtaaaac ctggtttgtg gaagcgaaac tgaaacaggg
     8281 cattgagagc gccactcaga aaaaaatcgt caagatgatc aaagacagca aactgaaagt
     8341 gcaggcgcaa attcagggcg atgagatccg tgtgacgggc aaatctcgtg atgatttaca
     8401 ggctgtcatg gcgatggtgc gcggtggcga tctcggtcag ccgttccagt tcaaaaactt
     8461 ccgcgattaa tcgcgacaac ctgcgctttg ttcatgtcag atgcggcatg aacgcctggt
     8521 tcggcctaca aagtttttta aatcaggaac ttagtaggcc tgataagcgt agcgcagcag
     8581 gcaatttcgc ctcgctttta tgcctggcga attgcctgtt caacatcaaa gcgattcgtc
     8641 actttgctgt cgattttcac ataagctgaa tgttcttctt ctgcaatcaa cacttctttg
     8701 atgccttcag tttccagcaa acgcactttt aacgcctcgt ttgcggcaat gttcgccggg
     8761 atttcaatgc gcaaactgct gacatacggc ggttctttca tggtactggc gactgccagc
     8821 cacactgcgg ccagcattgc gccagcgaga aataccccct gaccgtcaaa catgccgtca
     8881 atccagccgc ccagcgaacc gccaatcgcc acgccaagaa actggctggt ggagtaaaca
     8941 cccatcgccg tacctttgta acctgctggc gactctttac tgataagtga gggcaggagg
     9001 gcttccatca aattaaacgc cacaaagaaa agctgcacgc cgaccaccag ttgccagaac
     9061 tgcgtttgcg cgttcctcaa cacaatttcc gcaaccacga tcaacccgac gcagaagaca
     9121 aagacttgct tcattttgcg cttaacttca gcgtagataa tgaaaggcac gaccgagcca
     9181 aaggcgatta gcattgtcgc cagatagacc ttccagtgtt cagccgccgg gaaccccgca
     9241 tcagccagtt gtccgggcag ggcaacaaac gtcgacatca gcaaaatatg cagacacata
     9301 atgccaaagt tgagtttcag cagccgcggt tccgccagca ccttactgaa actgcctttc
     9361 accattccgg actcacgatt aagtacgtga gtgctactgt tgggcacaac ccaaatggtc
     9421 aacgcaatgc cggtcgttgc cagaatagcg atcatccaga acagcgcgtg cagcccaagt
     9481 ttgtgagtga tgatcgggcc aagcaccatc gcaatggcaa aggtaatgcc aaagctcacg
     9541 ccgataaacg ccattgcttt ggtgcggttt tgttcgcgcg tgagatcgga aagcagcgcc
     9601 ataacggcag cggcaatcgc accggagcct tgtagcgccc gacccagaat aattccccag
     9661 atggagtcag agagcgcagc gataacgcta ccggcggcaa acactgccag cccaccgaca
     9721 attaatggtt tgcgaccaat gcggtcggaa agcaggccaa acggaatctg aaaaacggcc
     9781 tgagtcagac cataaatacc aatggcaata ccgattaatg cttcgctggc accttgcaga
     9841 gccatgccgt acgtggtcag aaccggcaga accatgaaca tgcccagcat gcgcaacgag
     9901 aatacggtcc ctaaacccca ggtcgcgcgc cgctcacctg gcgtcatttt ataatcgttc
     9961 attaccacct ctgttttaaa ttcgcgacta gtgtaaagcg gcaaacgaat ggggtaaaca
    10021 tgcggatttt ttagtaaata tttcgagagg gatatgtttc taatgctaag aaaaaaggtg
    10081 ccgtagcacc tttttaatag agaggttgtg ttaccacaca gcagccagca gcgtatgcga
    10141 gtccggcacc ataaaatcaa cggacatcat caccgagagg gcagtgatgg cgatgataga
    10201 gaagccgaac agcttgcgcg cccagattct gtcatcagca actttataac cgcgcagagc
    10261 catacctaac caccagacgc taaccgccgc ggcgaccacc agatatttat atccagcgta
    10321 accgccaaga gagagcatca gcgtggcaac ggcaaaggcg atgatataca gcgtgatgtg
    10381 attcttcgcc accgaaatgc cttttaccac tggcaatacc ggaatgtttg ccgcctggta
    10441 atccttaaag cggaaaatgg cgatggcata ggagtgaggc atctgccaca ggctgaagat
    10501 agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt actgcacagt agccaatcac
    10561 cggcggcgca gcgccggaga gcgaaccaat caatgtgccg tagacggagt ggcgtttcat
    10621 gtacaggcta taaacgccga cataaaccac aaagcccatc acccccagcc agcaggccag
    10681 cggattcgcg ccaaaccaca gcagcataaa gccagcaata cccagcaagg tggcgtacac
    10741 cagcgagaca gcaggagaga tcaggccttt aaccagcacc cgattcttcg tcctttccat
    10801 ctttctgtcg atatccctgt cgatgtagtt gttaaacaca caacccgacg ccacaaccag
    10861 tgacacccca accagcgtgt agataaacag gggataatca atgctgccct ttgaggccag
    10921 caggaatccc ccaatcaccg agatcaggtt gccaaagatg atgcctggtt tcgttacttg
    10981 caggtattgc ttaaacatca taaccgccgc tcttagtgca tcatcatgtt gtagttgagg
    11041 ttccacataa tccagatgga gcctacaact aggatagcga tgattagcac ggtgaagaca
    11101 aacgccgtca tgttccagcc ttcatctgat ttggtattca tgtgcaggaa gcacaccaga
    11161 tgcaccagaa cctgtaccac tgccattgcc aggattgttc ccagaattac ggccggagag
    11221 gcagcccctg tcatcaccat ccagaacgga atcaccgtca gaatgatcga caggataaag
    11281 cctgtcatgt aggtttttac gctgccatgg gacgcgccgc tgtgatcggt agaatgactc
    11341 attacatcgc ccccatcaga taaacaacag tgaacacaca gatccaaacc acatccagga
    11401 agtgccagaa caggctcagg cacatgatgc gggtacggtt agtgctggtc aggccgcgac
    11461 gagcgatttg taccatcagc accgccatcc agataagacc ggaagtgacg tgcagaccgt
    11521 gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct gcgatccgga cccatgccgt
    11581 taacaatcag gtgatggaat tcgtagattt ccatcccgat aaatccggcg ccaaacaacc
    11641 aggtcaacgc cagccaggag ataacctggc ttttgttgtt tttgtacatg gcgatagccg
    11701 ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt ttcaaccaga acgaacggca
    11761 gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc gttcaccaga acggcatagg
    11821 tagcaaacaa gatagagaac agaatgcagt cgctcatcag gtagatccaa aatccgaaga
    11881 ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc gtggacagtc gcgtgcgtca
    11941 aagtatcagt tgccattttt cagccctgcc ttagtaatct catcgaaatg ctggttttcc
    12001 agtttttcga tttctgccac cggcacgtag taatccacgt cctcgtcgaa gcttttcacg
    12061 atccaggtga tgatcatgcc tgcgaagcca acaatcgcca gccaccagat atgccagatc
    12121 atggcgaaac cgaagatggt ggagaaagcc gcaatgacga tacctgcacc gctgtttttc
    12181 ggcatatgaa tttcttcata gtggtcaggc tttttatacg cttcgccttt ctctttcatt
    12241 tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg caaagttata gaacggaggc
    12301 ggggaagagg ttgcccactc cagcgtacgg ccaccccacg ggtcgccagt caggtcacgg
    12361 ttctggtcgc ggtcacgaat agaaacgtac atctgaataa cgaggcagag aatacctagc
    12421 gcaatcagta ctgcaccgct ggctgcaatc atcagcatgg tgtggaactg cgggtcaatc
    12481 tgctggctca aacgacgggt catgcccatg aagcccagcg cgtacagtgg cataaaggca
    12541 acgaagaagc cgatgatcca gaaccaaaac gcgcgtttac cccaggtttc gttcagtttg
    12601 aaaccgaacg ctttaggcca ccagtaggtc atccctgcga agcagccgaa gaccacgccg
    12661 ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc tgttatgtag aacgaagtcc
    12721 gcgcccggta ctgccagcag cacgccagtc ataccgccca ccgagaaggt gacgataaaa
    12781 ccgatagtcc acagcatcgc agaatggaac acgatgcggc cctgatacat ggtgaacagc
    12841 cagttgaaga tcttcacccc ggtcgggatg gcgataatca ttgtggtgat accaaagaag
    12901 gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt gcagccaaac gatgaacgac
    12961 agcacggtga tacagacggt tgcccatacc agcgaggtat aaccaaacag acgtttacgc
    13021 gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag gcaggatcag gatgtaaact
    13081 tccgggtggc cccaggccca aatcaggttg atgtacatca tcatgttgcc acccatatcg
    13141 ttggtaaaga aatgggtgcc cagatagcga tccagggtca acaacgcgac ggtaaccgtc
    13201 agaattggga aggaagcaat aatcagtacg ttcgcgcaca gtgatgccca ggtaaatact
    13261 ggcatcttga acatggtcat gcccggtgcg cgcatcttca gaatggtaac gaagaagttg
    13321 ataccggtaa gcgtcgtacc tataccggat agctggagac tccatatcca gtaatcgaca
    13381 ccgactcccg gactgtactc tattcccgat agcggtggat aggccagcca gccggtctgc
    13441 gcaaattcgc ccacgccgag agaaacgtta accagaatca caccaacaac ggtaaaccag
    13501 aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg cgccgatctg cagcggaacc
    13561 accaagttca tcagaccgat aacgaaaggc atcgccacga agaagatcat aatcacgccg
    13621 tgcgcggtga agatctgatc gtagtggtga ggtggcagga agcccgcttc gcccgccgag
    13681 gcaagagcct gctggctacg catcataatg gcgtcagcaa aaccacgcag caacatcaca
    13741 atcgccacga tgatatacat gataccgagg cgtttatggt cgacggaggt cagccactct
    13801 ttccacaggt aggtccactt accgaagtaa gtgatcaggc caacgagcgc cagacctccc
    13861 aaaataatgc cagcgatcgt aaccatgacg ataggttcat ggaacgggac tgcatcaagt
    13921 gataattttc cgaacatctt tattcttcct caaccccttt aatgggcgga ttccgcgtgg
    13981 ctcatgtcca tgccttccat accttcgtgt gcgctgtgct caccttctgg ctgggtcatg
    14041 tccatgctct taccgtgagc cataaacttg ttaattacat cggcaaacaa gtttggtttc
    14101 acgttggaga aatattccac ctggttgtat tcgctaggcg cggccagttt ttcgaacgct
    14161 gccatgtcag acatggagtt cggcgactgt ttcgcttttg cgacccactg gtcgaatgcg
    14221 gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg agaagcccgg gccgctgtag
    14281 ctggcggaga taccgtcata agtgccgggt tcgttggcga tcagatgcag gcgagtctgc
    14341 ataccggcca tggcataaat ctggctaccc agacgcggaa tgaagaagga gttcatcacg
    14401 gagttggagg tcactttgaa gtacaccgga gtgttcgccg ggaaagcgat ttcattcacg
    14461 gtagcaaagc cctgttccgg gtagatgaag aaccatttcc agtccatgga aaccacttcg
    14521 atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag gctcaagagc gtgagtggtt
    14581 ttccaggtca gtaccgcaag gaagatgatg attaagatag gtaccgtcca gaccacagct
    14641 tccactttat tggagtgtga ccagttcggg ctgtacttag catctttatt gctcgcacgg
    14701 tacttccagg cgaaaccaac agccatcaag attgcgggaa taacgacaat caacatcagg
    14761 ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa tctgtccttt gggatctaac
    14821 agcgcagaat tacagccact gagcaataca gtgcctgcaa ataatgacaa ccatcccaaa
    14881 cttttattgt atttcctgag tctcatttaa cgacctcaat tccacgggat ctggtggcgt
    14941 ttaaaatgtg tgggcatttt acgggaaggt tacattactg taaacatgat taaattcctg
    15001 ttacttggat ttggctgtca ggtcacatat gttgcaaaac atatcaggtt gattttaaga
    15061 agctgttgca aaagggcgtt ataacaaaag gggagtgcag aagaagtctg gacgggcaat
    15121 tggtataatc aatgcgaaat aaaacaatta tttaacaaat aattatcatt ggagtgacaa
    15181 cttggtggtg agaaaataaa gactcattag ctgaatcgtg taagaaaaat agaatttat
//
LOCUS       DAZKFZ010000101        15137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000101 DAZKFZ010000000
VERSION     DAZKFZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..508
                     /locus_tag="ACHOQW_002834"
     CDS             <1..508
                     /locus_tag="ACHOQW_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9573885.1"
                     /translation="KKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTV
                     NIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAV
                     GAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQP
                     NTKCPEKS"
     gene            524..1063
                     /locus_tag="ACHOQW_002835"
     CDS             524..1063
                     /locus_tag="ACHOQW_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP9573886.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1156..2733)
                     /gene="guaA"
                     /locus_tag="ACHOQW_002836"
     CDS             complement(1156..2733)
                     /gene="guaA"
                     /locus_tag="ACHOQW_002836"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9573887.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2802..4268)
                     /gene="guaB"
                     /locus_tag="ACHOQW_002837"
     CDS             complement(2802..4268)
                     /gene="guaB"
                     /locus_tag="ACHOQW_002837"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9573888.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4430..5800
                     /gene="xseA"
                     /locus_tag="ACHOQW_002838"
     CDS             4430..5800
                     /gene="xseA"
                     /locus_tag="ACHOQW_002838"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9573889.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5797..6012)
                     /locus_tag="ACHOQW_002839"
     CDS             complement(5797..6012)
                     /locus_tag="ACHOQW_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9573890.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6081..7553)
                     /gene="der"
                     /locus_tag="ACHOQW_002840"
     CDS             complement(6081..7553)
                     /gene="der"
                     /locus_tag="ACHOQW_002840"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9573891.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7671..8849)
                     /gene="bamB"
                     /locus_tag="ACHOQW_002841"
     CDS             complement(7671..8849)
                     /gene="bamB"
                     /locus_tag="ACHOQW_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP9573892.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8860..9480)
                     /gene="yfgM"
                     /locus_tag="ACHOQW_002842"
     CDS             complement(8860..9480)
                     /gene="yfgM"
                     /locus_tag="ACHOQW_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP9573893.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9498..10772)
                     /gene="hisS"
                     /locus_tag="ACHOQW_002843"
     CDS             complement(9498..10772)
                     /gene="hisS"
                     /locus_tag="ACHOQW_002843"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9573894.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10883..12001)
                     /gene="ispG"
                     /locus_tag="ACHOQW_002844"
                     /gene_synonym="gcpE"
     CDS             complement(10883..12001)
                     /gene="ispG"
                     /locus_tag="ACHOQW_002844"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP9573895.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12028..13041)
                     /gene="rodZ"
                     /locus_tag="ACHOQW_002845"
     CDS             complement(12028..13041)
                     /gene="rodZ"
                     /locus_tag="ACHOQW_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP9573896.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13326..14480)
                     /locus_tag="ACHOQW_002846"
     CDS             complement(13326..14480)
                     /locus_tag="ACHOQW_002846"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP9573897.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14630..15061)
                     /gene="ndk"
                     /locus_tag="ACHOQW_002847"
     CDS             complement(14630..15061)
                     /gene="ndk"
                     /locus_tag="ACHOQW_002847"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9573898.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
BASE COUNT         3672 a         4168 c         3751 g         3546 t
ORIGIN      
        1 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
       61 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
      121 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
      181 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
      241 cggccacaac gtcgggtctg gcagcaattc cggaacgacg gcaggtgcag ttggcggcgg
      301 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
      361 tttaacgtat aaggaaggca ccaaagtgta tacctctact caggtgggta aagagtgcca
      421 gtttacgaca ggtttagccg ttgttattac cacgacgtat aacgaaacgc gtattcagcc
      481 aaataccaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
      541 ttgcgccatc ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
      601 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
      661 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
      721 ggctgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
      781 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
      841 cagcttagag attcagaaac aaaaactggc gctggcaaaa gaagaagccc gcgttaagcg
      901 agaaaacgaa tttatcgatc aggagctgaa gcacaaagcc gcgcaaactg atgtcgtgca
      961 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
     1021 gggcaaagca gaagagaata aatcggacag ctggtttaat taatcgatgt tagtaacttc
     1081 aagcctataa ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
     1141 tagtgcaggg tcaaatcatt cccactcaat ggtagctggt ggcttgccgc tgatgtcata
     1201 caccacgcgg gaaataccgt tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa
     1261 atcgtacggc agatgcgccc agtgtgcggt cataaagtcg atggtttcga cagcacgcag
     1321 agagacaacc cagtcatact tacgaccatc gcccattacg ccaacggaac gtactggcag
     1381 gaagacggtg aacgcctggc tgactttgtc gtacaggtcc gctttacgca gttcttcaat
     1441 gaagatggcg tcagcacggc gcagcaggtc acagtactct ttcttcactt cacccagaac
     1501 acgaacgcca aggcctggtc ccgggaacgg gtgacggtac agcatgtcgt acggcaggcc
     1561 cagctccaga ccaatcttac gcacttcgtc tttgaacagc tctttcagcg gttcaaccag
     1621 gcccatcttc atctctttcg gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc
     1681 tttaccggtt gcagacgccg cagattcgat aacgtcaggg tagatggtgc cctgcgccag
     1741 ccacttcacg tcttccagtt tcagcgcttc ttcatcgaat acttcaacga aaacgcgacc
     1801 gatgatttta cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc
     1861 ttctgccggt acgtgaacaa tgttaagacc aaagtgatcg ccaaacatat ccagaacctg
     1921 ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc
     1981 gatagcgcgg tgcagcagca ttgcggttac ggaggaatcc acaccaccag agaggccgag
     2041 gatgacttta tcgtcgccta cctgctcgcg gatgcgagct acagcatcgt cgataatttt
     2101 cgctggcgtc cacagggctt cacactggca gatatcacgc acaaaacgct ccagcatgcg
     2161 cataccctgg cgggtgtgag tcacttccgg gtggaactgt acgccataga agcgtttttc
     2221 ttcgttagcc ataatggcaa acgggcagct ttcggtgctg gctacggtga cgaagtcgga
     2281 cgggatagcg gtaactttat cgccgtggct catccagaca tcgagcagcg gtttaccgtc
     2341 tgcggtcagc gcatcttcga taccgcgaac cagtgcgctg tcgtttacga cttcaacctg
     2401 cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga ccgcccaact gcattgccat
     2461 ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga catactgcgg
     2521 cgcacgcggg ctgttttctt cagtagtact ttccgggccg ccggaaagaa taatgccgct
     2581 tggattgaag tcacgaattt gtgcttctgt cacatcccat gcccacagtt cgcagtaaac
     2641 acccagctca cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga agtccagaat
     2701 gaggatgcga tgcttatgaa tgttttccgt cattgacgct tattccgagg caagtgaaac
     2761 agataatata aatcgcccga catgaagtcg ggcgaagaga atcaggagcc cagacggtag
     2821 ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg
     2881 ctgatacgta caaactccgc tttagtacgc agttcgtcga tagtaccaca gccggtcaaa
     2941 cccatacagg agcgcaggcc gcccatctgc tggtgaatga tctctttcag gcgaccttta
     3001 taagctacgc gaccttcgat accttccggc accagtttgt cggcagcgtt atcgctctgg
     3061 aaataacggt cagaggaacc tttggacatc gcgcccaggg aacccatacc acggtaagat
     3121 ttgtaagaac ggccctggta gagttcgatt tcacccggag attcttcagt acccgccagc
     3181 atggaaccta ccatcaccgc gcttgcgcca gcggcgatag ctttggcgat gtcgccggag
     3241 aagcgaatac caccatctgc gataaccgga ataccggtgc cttccagggc ttcaactgcg
     3301 tcagcaacgg cagtgatctg cggaacacct acgccagtaa cgatacgagt cgtacagata
     3361 gaaccagggc cgataccgac tttaaccgcg ctgcaaccag cttctgccag agcgcgtgca
     3421 cctgcagctg ttgccacgtt gccgccgatg atttgcagat ccggatattt agcacgggtt
     3481 tcacggatac gctgcaaaac gccttctgag tgaccgtggg aggagtcgat cagcagaacg
     3541 tcaacgccag cggcaaccag cgcgtcaaca cgctcttcgt tacccgcacc tgcgccaact
     3601 gcagcaccaa cacgcagacg gccttgctcg tctttacagg cgttcggttt acgttccgct
     3661 ttctggaaat ctttcacggt gatcatgccg atcaggtgga attcgtcatc aaccaccagc
     3721 gctttttcaa cgcgtttttc gtgcattttt gccagcacca cttcacgggc ttcaccttca
     3781 cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg
     3841 gtaacaaaac gcacgtcacg accggtgata atccccacca gttcgttttc ttcggtaacg
     3901 accggatagc ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg
     3961 gttggcagaa cagtctgcgg atcagtcacc acaccagatt cgtgtttttt cacacggcga
     4021 acttcttctg cctggcgttc aatggacatg tttttgtgga taaagccgat accgccttcc
     4081 tgagccagag caatagccag gcgcgcttcc gttacggtat ccattgctgc ggaaagcata
     4141 gggatattca gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt attcggcaga
     4201 acggtagaat gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc tttagcgata
     4261 cgtagcatgg gcaatatctc gaccagagtg gttaataaat attgccgcgg cattatacag
     4321 agcgtaaccg attgcatcta cccctttttg caaaaaatgc ttgctatccc cgaagggcgg
     4381 gttactatcg actgaataac ctgctgattt agaatttgat ctcgctcaca tgttaccttc
     4441 tcaatcccct gcaattttta ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga
     4501 gcatgagatg ggacaggttt ggatcagcgg cgaaatctct aatttcacac aaccggcttc
     4561 cggtcactgg tactttacac tcaaagacga caccgcccag gtacgctgcg cgatgttccg
     4621 caacagcaac cgccgggtga ccttccgccc acagcatggg caacaagttt tagttcgcgc
     4681 caatattacg ctctacgagc cgcgcggcga ctaccagata atcgttgaga gtatgcagcc
     4741 ggccggtgaa gggctgctgc aacagaagta cgaacagctc aaagcgaagt tgcaggctga
     4801 aggattgttc gatctgcaat acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt
     4861 gatcacctca aaaaccggtg ctgcgctaca tgatattttg catgtgttaa aacgtcgcga
     4921 tccttctctg ccggtgatca tctaccccac cgccgttcag ggcgatgacg cgccggggca
     4981 aattgttcgc gccattgaac tggcgaatca gtgcaatgag tgcgacgtgt tgatcgttgg
     5041 gcgcggcggc ggttcgctgg aagatttatg gagttttaac gacgaacgcg tagcgcgggc
     5101 gatttttgcc agccgcattc cggtcgtcag cgccgtcggg catgagacgg atgtgaccat
     5161 tgccgatttt gttgccgatc tgcgtgcacc aacaccgtcg gctgccgccg aagtggtgag
     5221 ccgtaatcag caagagttac tgcgccaggt gcaatcgacc cgtcaacggc tggagatggc
     5281 gatggattat tatctcgcca accgcacacg tcgctttacg caaattcatc accgattaca
     5341 gcaacagcat ccgcagctcc ggctggcacg ccagcaaacc atgcttgaac gcctgcaaaa
     5401 acggatgagc tttgcgctgg aaaatcaact taagcgtgcc gggcaacagc agcagcgatt
     5461 aacacagcgg ctgaatcagc aaaatccaca gccgaagatt catcgcgcgc aaacgcgcat
     5521 tcagcaactg gaatatcgtt tagcagaaac cctgcgcgca cagcttagcg ccacgcgtga
     5581 acgtttcggt aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg
     5641 tggatacagc gttactactg ctactgacgg caaggtactg aaaaaagtga agcaggttaa
     5701 agcgggtgaa atgctaacca cacgtctgga agacggctgg gtagaaagtg aagtaaaaaa
     5761 catccagccg gtaaaaaaat cgcgtaaaaa agtgcattaa gccagcacga attcaacgcg
     5821 ttttttcgat atcaagccat gcccgtgctg acagaagtaa tctaccgcac cgcaggcttt
     5881 taacacctgc aacggttgat ggcagtccgg gcaaagagct ttcatttcaa taactttccc
     5941 gcaggatggg caatacgcat ggccgttatc ctgatcaagc acatgctgac attgtggaca
     6001 atgcagttcc atatgaattc ctccggtagc cacccctcta cattcataga gggaatggca
     6061 gataaaatac ttacggataa ttatttattt ttcttgatgt gcttcatcag acgcttacgt
     6121 ttacgcatct gggttggcgt cagggtgtta cgcttgttcg catacgggtt ttccccttct
     6181 ttgaactgaa tacgaatcgg cgatcccatt acgtccagcg atttgcggaa gtagttcatc
     6241 aagtaacgct tgtaggaatc aggcaggtct ttcacctgat taccgtgaat caccacaatc
     6301 ggcggattat aaccaccggc gtgtgcatat ttcagcttca cacgacgacc gcgtaccagc
     6361 ggcggttggt gatcttcaac agccatcgtc atgatgcgcg tcagcataga ggtccccaca
     6421 cgacgggtgg agctgtcata cgcttcacgt actgattcaa acaagttacc aacaccactg
     6481 ccgtgcaagg cagagataaa gtgcacacga gcaaaatcga taaagcccag acggaagtcc
     6541 agcgtttctt tcacctgctc tttcacttcc tgactcaggc catcccactt attcaccaca
     6601 atgacaagtg agcgcccact attgagaata aagcccagca gcgagagatc ctgatcggaa
     6661 ataccttcgc gcgcatcaat cactaacatc accacgttgg cgtcttcaat ggcctgcaac
     6721 gttttgatta cggagaattt ctctacagca tcggtgattt tgccgcgttt acgtacgcca
     6781 gcggtgtcaa tgagcacata ctcacgtcca tcgcgttcca ttggaatgta gatactgtca
     6841 cgcgtcgtac caggcatgtc ataaacaaca acgcgctctt caccaagaat acggttagtg
     6901 agtgtagact tacctacgtt cggacgaccc acaatcgcca gtttgatcgg cagactttgc
     6961 gggtcgaagt cgtcttcctc ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag
     7021 tattcagcgt cttcgtcgac ttcctcttgc ggtgcgagat cttccatcca cggcagtagc
     7081 acatgctcca gcagacttaa tacgccacga ccgtgagacg cggcgatcgg gtagatttca
     7141 cctaaaccaa gcgagtagaa atcaaccact gcctgatcgg gatccagacc gtcagttttg
     7201 tttgccacca ggaaggtcgg tttttcacgg gagcgcagat gtttggcaat cgcttcatct
     7261 gccggcatca ggcccgcgcg cgcatccacc ataaacagta cgacgtccgc ttcttcaatc
     7321 gccagcagcg actgttccgc catgcgggtt tctacaccgt cttctgtgcc atcaatcccg
     7381 ccggtatcaa tacagataaa ctcacggcct tcaatttccg cacgaccgta cttacggtca
     7441 cgagtcagac ccgggaaatc cgcaaccagc gcatctcggg tgcgagttag acggttaaat
     7501 aacgtggatt ttcctacgtt agggcgcccg acaagcgcga ccacaggtac catgtttaaa
     7561 gcctcatttt tataaatcat cagacaacgc acgctatatt cgcgtcgttg ttaaaaacag
     7621 gaaaacggcc cctgtccagg agccgttttc aaagtgaacg acagagacga ttaacgtgta
     7681 atagagtaca cggttccgtc ttttgcctgg atcagcagtt tgccgtcagc ggcaaccggt
     7741 tcagtctgga aaccggaact atcaactttt tgctgggcaa cgaaacgacc atcttcgacg
     7801 ttaatccagt gcaaataacc ttcactgtca ccgaccacca ggttgccatt atacagcacc
     7861 ggagaagtca gcaggcgatg cagcagatcg ctttgtgtcc acagcgtaac gccgccatca
     7921 atggtcaacg ccatcacccg gtcattttga tcgaccagat agatgcgatt gccgtcgacg
     7981 atgaaatcat tcaccgaacc cagttcgcgt ttccacataa tctgaccact gcgcagatca
     8041 agcgccgtca ggttaccatt ataggccagc gcgaaaacaa cgccgttaac aacggcggga
     8101 gtcgtgtcaa catcgctcag acggtcaatt tcggtagaac cggtcgcttg ggaaatacgc
     8161 tgctgccaaa tcatctggcc ctgttccatc agcactgcgc tgacgcgacc attatcgccc
     8221 cccacgacgg ccgcaccaaa agccgttgcc ggcgcagact cgccacgcaa agagagcgaa
     8281 ggcatatcga ggttaactgt ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac
     8341 tgaccgttac tggtgtggat taacaccaga ccgtcgctga ccaccgggcg cgaaagtgct
     8401 tcacccgcga ctttagtttg ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc
     8461 tgcgcctttt cgctgccaat gtagacatgt ccaccagaca cggtcacacc gccagaaagt
     8521 aatgcaggct ctttagagaa ccagccatct ttctcggcca ggctgacaga ccagatttct
     8581 ttgccatcat ccgcattcag cgcttttact aaaccagcgc ggtccgctgc atagacaacg
     8641 ttgtccgcca gtgccggatg aagattggaa tagaagttgc caatgccgct accaacggaa
     8701 gtgctccacg ccgtggtcgg cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt
     8761 accacatctt cttcgctgtt aaacagcgaa cagccgctta aaagggtaac ggaaagcagt
     8821 cctggcagca gtaatttacg caattgcatc gggtccctct cagatggaca aattattaat
     8881 tttcatctgc atcatttcgc tcagtgccgg agtaacatcg cttttcacgc ctgcttccca
     8941 tgcactacgc gcaccttgct tatcaccttt gctcagcaat gcttcaccac gcaggtcggc
     9001 aacaatggca gcccaccctt cacctttgat ggtatcaagg gttttcagcg cggcatcagc
     9061 ctgcttgagc tgtacctgaa cgcgagcaag acgcagattt atcacggctt tgagattttc
     9121 atcgctcgtg tctgccagcc cctgttgtaa ctgggcggca gctttctcta gttcattttt
     9181 gtcaacaaat tgctgcgcaa gttccaaaga agccagcgca ccataagtat ttttattttc
     9241 agcagcaaat ttttccgccg ccgggatgct atccggtttg ccttcgctca ctgcggtaac
     9301 cgcattttga taggcaagag aagcggagcg tgcagaatca acctgatggc tgttccagta
     9361 gcgccagcca atcagtgcgc caacgcccaa aatcacccca acagccagtg ctttgccatt
     9421 ttcagcaaaa aagcgtttaa tcgcttctac ctggtcgttt tcgttctcgt aaatttccac
     9481 gctgtccttc tccttcctta acccagtaac gtgcgcaaat gcgcggctac gctatcctgc
     9541 gcaactgccg tttgctcacc agagcgcaaa tccttcacta ctgctgtgcc gttagccact
     9601 tcagactcac ccagcaccac agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt
     9661 ttcttaaagt tgccgccgcc gtggttggtc atcaatttca cgcccggtaa ttcatcacgc
     9721 agacgctcag ctaatgccat agccgcagat tgtgtatcag cacctgaagc caccaggtat
     9781 atatcgacaa caggatcggc tttaaattcc ggattaacgg cctgtactaa caatacaaga
     9841 cgttcgaggc ccatcgcaaa accgacagcc ggtgttgcac ggccgcccag ttgttccaca
     9901 agaccgtcat aacgaccgcc tgcacacacg gtgccctggg agccgagact gttagtcacc
     9961 cactcgaaaa cggtacggtt gtagtaatcc agaccacgca ccagacgctg gtttacggtg
    10021 taagcgatcc ccgcgctctc aagcagtttg cacagaccgg caaaatgctc acgagattcc
    10081 tcgtccagat aatcacctaa tgccggagcg tcgttgagaa gcgcctgcac ttccggattt
    10141 tttgaatcca gcacgcgcag cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc
    10201 ttttctttat gctgctcaag gaatgccacc agcgcatcgc ggtaattggc gcgtgcttcc
    10261 agcgaaccga tagaattcag ctcaagagtt acgtgttcgg aaatacccag cgcgcgccac
    10321 cagcgggcgg tgagcataat cagttcagcg tcgatatccg gaccttgcag accgaaaact
    10381 tcgcagccca actgatggaa ctgacgataa cgccctttct gcggacgctc gtgacggaac
    10441 atcggcccga tataccacag acgctgttcc tgattgtaca gaagaccatg ctcgatgccg
    10501 gcgcgtacac agcccgccgt cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc
    10561 tcaaaggtgt acatctcttt ttcaaccacg tcggtgactt caccaatcgc acgtttgaat
    10621 agcggggtct gctctacaat cggcaagcgg atttcactgt aaccgtagct gccgagcacg
    10681 tttttcagtg tgccttcaat gcgctgccag atggccgttt cgccaggcag gtaatcgttc
    10741 atgccgcgaa tggcttgaat gttttttgcc acgtttattc tctttctgaa tataaaaatg
    10801 aaccctcaac gcttccctca atgtttcggg agccatgcgg gttcaatcat acacgggaag
    10861 cgaggcgctt cccatcacgt tattattttt caacctgctg aacgtcaatt cgacgcgctt
    10921 cgtccagctg actggctttc gcacgaatgc gtgcctccag ctggtcgatc atatcgttgt
    10981 tgtccagacg gtctttgcgc acgccatctt catagagacc gcttttcttg ttgccgccgg
    11041 tgacgccgag tgtagaaacc agcgcctcac ccgggccatt caccacgcag ccgataatcg
    11101 aaacgtccat cggagtgatg atatcttcca ggcgttgctc cagcgcgtta actgtaccga
    11161 taacatcaaa ttcctgacgc gaacaggtcg gacaggcgat gaagttgata cctcgcgaac
    11221 ggatacgaag cgatttcaaa atatcgaaac cgactttgat ctcttcgacc ggatcggccg
    11281 ccagtgatac gcgcagcgtg tcgccgatgc cttcagacag cagcagacct aaaccaatgg
    11341 cggattttac tgccccgctg cgcgcgccac ccgcttcggt gatccccaga tgcagcggct
    11401 gatcgatttg ttttgccagc aaacgataag actcaacagc gaggaagacg tcagacgctt
    11461 tcacgctgac tttgaactga tcgaagttca ggcgatcgag atgatcaaca tggcgcatgg
    11521 cggattccag caacgcctgc ggcgtcggtt cgccatactt ttcttgcaga tctttttcca
    11581 gcgatccggc gttaacgcca atacggatcg gaatgttttt atcgcgcgca cagtcaacca
    11641 ccatgcgaat acgctcttca ttaccgatat tgccagggtt aatacgcaga caatcgacgc
    11701 cgtattccgc tactttcagc gcaatgcgat agtcgaagtg gatgtcagcc accagcggca
    11761 cgttaacctg ctgtttgatg agtttgaacg cttctgccgc gtccatcgtc ggtacggaga
    11821 cacggacgat atcagcgcca acgcgttcca gcgccttgat ttgattgacc gttgcttcga
    11881 catctgtcgt acgcgtattg gtcatggact gtacggcgat gggagcacca tcgccaatcg
    11941 gcacattccc aacgtaaata cgtgttgatt ttctacgttg aattggagcc tggttatgca
    12001 tgaaaaatct cccgcgttac ccgtctgtta ctgcgccggt gattgttcgg cattgagggt
    12061 cagacgcgca acctggttag ttctgataaa acgactcaga tcgacaggtt tcccttgata
    12121 ctggatctgt actgcggctg gcgcaccaat tttcagtttg tacggtgcct ggccggttaa
    12181 gttcaaatta ccgtctttac gctgcatacc gctaaacaat tttttaccgg tagcatcagt
    12241 gacctccagc cagcaatcgg cagtaaagtt catcaccagc gcattcggat cagccaccgg
    12301 cgtggtcacg ccagcctgat cggttggcaa cggcgcagca ccatctggcg ttgttgctgc
    12361 cgttggtgcc ggggtcgcgg cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt
    12421 ctgttgcgga tcaacagctg gtgctggcgc agttacgaca ggtgtttgcg tgttggttgc
    12481 ggtagtatcc acagacgctg gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt
    12541 atttaacgga acactctgcc cctgctcgct attactgctc agttccgccg aagattgatc
    12601 ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct
    12661 caggccgata accacaaaca acaccagcca ggtgaaggtc atcagccagc cgtcgcgttt
    12721 tttgcggcgt ttaccgaggg aaaaactctg catcggcgca acttttgcag cccgtagtgg
    12781 agcctgcttt tccagccctg gcagcagttc ttcttctgga atatgtacca gacgcgcata
    12841 agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc
    12901 aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gctgactaag
    12961 tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc
    13021 gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt
    13081 gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta
    13141 taagaccgtc tgtgtgctcg tgacaaagcc cacattcact aaggctaact tactgttgca
    13201 tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc
    13261 aacttaatac ttcattcata aaacattacg ccacggtaca taaagtaacc gtggcgtaat
    13321 ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg cagggtacgt
    13381 ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcgat atcatcacca
    13441 cgcgttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg
    13501 atacggctgt tcgagctgcg tccatacggc gcagccggga acgggttcca cgggatcagg
    13561 ttgatcttac acggcgtatc tttcagcagt tccgccagtt ggtgcgcgtg ttcagtgccg
    13621 tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt ggcgttggat
    13681 ttctccagat aacggcgcac cgcagcaagg aacgtttcga tattgtactt tttgttgatc
    13741 ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc cagcgcaaca
    13801 tcgatcatat cgcccagttt atccagcgct ggaactacac cggaagtgga aagcgtcacg
    13861 cgacgtttag acagaccaaa accgaaatca tcaagcatga tttccatcgc cggaacgacg
    13921 ttgttcaggt tgagcagcgg ctcgcccatg cccatcatca ctacgttagt gattgggcgc
    13981 tgaccggtga cttttgctgc gccgacgatt ttcgccgcac gccacacctg gccaataatt
    14041 tccgataccc gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa tttacactcc
    14101 agcgcacacc ccacctgcga agagacgcag agcgtagcgc ggtcgtcttc cgggatgtac
    14161 accgtttcga cacgctggtc acccacggca atcgcccatt taatggtgcc gtcagatgaa
    14221 cgctgttctt caaccacttc cggtgcgcgg atctccgcca cctctttcag tttgccgcgc
    14281 aacactttgt tgatgtcggt catctcatca aaattgtcgc agcaatagtg atacatccac
    14341 ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc
    14401 tgctgacgat tgagatccag caggttgatt tttccatctt tggtggtgac gttttcaggt
    14461 gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg
    14521 agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac
    14581 aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc
    14641 gcgggcacac ttcgccttcg ccgaagaaat aagcgatttc gcgagcggca gattcgacgg
    14701 aatcagaacc gtgggtaccg ttttcggtca ggctgtcagc gtaatcagcg cgcagagtac
    14761 cggccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt
    14821 tttcaccttc cagcacggaa accacgatcg ggccagaggt catgaattca accagaccat
    14881 caaagaacgg ttttccatcg tgttcagcat aaaagccacg tgcctgttca acggtcaggt
    14941 gcagcatttt ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca aagatattac
    15001 caatgacgtt ttttgctacc gcgttcggtt tgataatgga aaaagtacgt tcaatagcca
    15061 tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt
    15121 ataatgagca acagagc
//
LOCUS       DAZKFZ010000102        14985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000102 DAZKFZ010000000
VERSION     DAZKFZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(187..1218)
                     /gene="rsmC"
                     /locus_tag="ACHOQW_002848"
     CDS             complement(187..1218)
                     /gene="rsmC"
                     /locus_tag="ACHOQW_002848"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP9573899.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1321..1734
                     /gene="holD"
                     /locus_tag="ACHOQW_002849"
     CDS             1321..1734
                     /gene="holD"
                     /locus_tag="ACHOQW_002849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP9573900.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1703..2149
                     /gene="rimI"
                     /locus_tag="ACHOQW_002850"
     CDS             1703..2149
                     /gene="rimI"
                     /locus_tag="ACHOQW_002850"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9573901.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2164..2841
                     /gene="yjjG"
                     /locus_tag="ACHOQW_002851"
     CDS             2164..2841
                     /gene="yjjG"
                     /locus_tag="ACHOQW_002851"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP9573902.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            2932..4521
                     /gene="prfC"
                     /locus_tag="ACHOQW_002852"
     CDS             2932..4521
                     /gene="prfC"
                     /locus_tag="ACHOQW_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9573903.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4914..5519
                     /gene="osmY"
                     /locus_tag="ACHOQW_002853"
     CDS             4914..5519
                     /gene="osmY"
                     /locus_tag="ACHOQW_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP9573904.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5646..5807
                     /locus_tag="ACHOQW_002854"
     CDS             5646..5807
                     /locus_tag="ACHOQW_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP9573905.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            5929..7002
                     /locus_tag="ACHOQW_002855"
     CDS             5929..7002
                     /locus_tag="ACHOQW_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP9573906.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            6999..7781
                     /locus_tag="ACHOQW_002856"
     CDS             6999..7781
                     /locus_tag="ACHOQW_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9573907.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(7995..8858)
                     /locus_tag="ACHOQW_002857"
     CDS             complement(7995..8858)
                     /locus_tag="ACHOQW_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP9573908.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(8830..10379)
                     /locus_tag="ACHOQW_002858"
                     /pseudo
     CDS             complement(8830..10379)
                     /locus_tag="ACHOQW_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            10637..11416
                     /gene="deoC"
                     /locus_tag="ACHOQW_002859"
     CDS             10637..11416
                     /gene="deoC"
                     /locus_tag="ACHOQW_002859"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9573909.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11543..12865
                     /gene="deoA"
                     /locus_tag="ACHOQW_002860"
     CDS             11543..12865
                     /gene="deoA"
                     /locus_tag="ACHOQW_002860"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP9573910.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            12917..14140
                     /gene="deoB"
                     /locus_tag="ACHOQW_002861"
     CDS             12917..14140
                     /gene="deoB"
                     /locus_tag="ACHOQW_002861"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9573911.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14197..14916
                     /gene="deoD"
                     /locus_tag="ACHOQW_002862"
     CDS             14197..14916
                     /gene="deoD"
                     /locus_tag="ACHOQW_002862"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9573912.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3549 a         3919 c         4064 g         3453 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc ctgatgtgta attgctggaa aaatgtccat caggagacta
      121 acgtgcaggt tttgtaagtc ggataaggcg ttcacgccgc atccgacacg gtattcggcg
      181 agataattaa cctttcttcg cctggcgggt cataatggcg cgatacacct tgaagcgccc
      241 tgtttgcgcg atcacttcgt ggaagccaaa tgtctcatcc agcacgtccg gataaggcag
      301 gaaggcgttc gctacaattc gcagctcgcc gccgctatta agatgacgca ccgcaccgcg
      361 aatcagcgtt tgcgccgcat ccaggctggt ttgcatccca tcgtggaacg gcgggttgga
      421 gatgatcata tcaaaacgac ctttcacctc ggaaaagacg ttgctggcaa agacttcacc
      481 ttcaacaccg ttggccgcaa gtgttgcgcg gctggcttct accgctggcg cagagacatc
      541 gcacaaggtg agacgaattt tcggcgaatg gcgcgcaaag gcaaccgaaa gcacgcccgc
      601 gccacagccg acatccagca ctttaccttt cgtgtgcggg gttaacgtcg agagcagcaa
      661 ctggctaccg acatccagac cgtcgcggct aaacacgcca ggcagcgttt tgaccgtcag
      721 gccatcgacg ctgtattcgc cccagaattt atcggcatca aataccggct gtttttccag
      781 acgaccaaaa tagaggccac agcgacgggc gctgtcgact ttattcaacg gcgcataatc
      841 agccagcatc tgctcggcgc tgcgcacgcc gctgcggttc tcgccaacaa caaaaatatc
      901 cgtccccacc ggaagcagag aaagtaaatt catcaactgg aactgggctt ccggtttgtt
      961 cttcggccag tagtaaatca gcgtatcgca atctgcgaca tcatccgccg tggcgaccag
     1021 actaaaacgg gcgttatccc ccatctggcg gcttaacacc tgccaatgct ggaattgctg
     1081 ggtatgagca cggctggccg cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc
     1141 ggcaaacaga atacggcttt gttcgaaatc atcactgtga cgcagcaaga cttcacttgc
     1201 cggggtaaat gcagacatgg aatgctcctc aattgatact ggcggcgatt atagccatat
     1261 gttggcgcgg tatcgacgaa tttgctatat ttgcgcccct gacaacagga gcgattcgct
     1321 atgacatccc gacgagactg gcagttacag caactgggca ttacccagtg gtcgctgcgt
     1381 cgccctggcg cgttgcaggg ggagattgcc attgcgatcc cggcacacgt ccgtctggtg
     1441 atggtggcaa acgatcttcc cgccctgact gatcctttag tgagcgatgt tctgcgcgca
     1501 ttaaccgtca gccccgatca agtgctgcaa ctgacgccag aaaaaatcgc gatgctgccg
     1561 caaggcagtc gctgcaacag ctggcggttg ggtactgacg aaccgctatc actggaaggc
     1621 gctcaggtgg catcaccggc gctcaccgaa ttacgggcaa acccaacggc acgcgccgcg
     1681 ttatggcaac aaatttgcac atatgaacac gatttcttcc ctcgaaacga ctgatttacc
     1741 agcggcttac cacattgaac aacgcgccca cgcctttccg tggagtgaaa aaacctttgc
     1801 cagcaaccag ggcgagcgtt atctcaactt tcagttaacg caaaacggca aaatggcggc
     1861 gtttgcgatt acgcaagtgg tgctggatga agctacattg ttcaatattg cggtcgatcc
     1921 tgactatcag cgtcagggat tgggaagggc gctgctggaa catctgatcg acgaactgga
     1981 aaaacgcggc gtggcgacac tatggctgga agtccgtgcc tcaaatgctg ccgccattgc
     2041 cctgtacgaa agtttaggct ttaacgaggc gacgattcgc cgcaattact accccaccac
     2101 ggacggtcgc gaagacgcca tcatcatggc gttgccaatc agtatgtaag acaaggtgga
     2161 ataatgaagt gggactggat tttctttgat gccgatgaaa cgctgtttac ctttgactca
     2221 ttcaccggcc tgcagcggat gtttcttgat tacagcgtca cctttaccgc tgaagatttt
     2281 caggactatc aggccgttaa caagccactg tgggtggatt atcaaaacgg cgcgatcact
     2341 tcattacagc ttcagcacgg gcggtttgag agctgggccg aacggctgaa cgtcgagcca
     2401 ggtaaactca acgaagcctt tattaatgcg atggcggaaa tctgcacgcc gctgccgggc
     2461 gcggtttctc tgcttaacgc cattcgtggc aacgccaaaa tcggcatcat caccaacggc
     2521 tttagtgcct tgcaacaggt gcgtctggaa cgcacgggcc tgcgtgatta cttcgatttg
     2581 ctggtgattt ccgaagaagt tggcgttgcc aaaccgaata agaaaatttt cgattatgcg
     2641 ctggaacagg cgggcaatcc tgaccgttca cgcgtgctga tggttggcga cactgccgag
     2701 tccgatattc tcggtggcat caacgccggg cttgcgacct gctggctgaa tgcacaccat
     2761 cgcgagcaac cagaaggcat cgcgcccacc tggaccgttt cttcgttgca cgaactggag
     2821 cagctcctgt gtaaacactg attgcctccc ccccgttgat gggtaaaata gccgcaattt
     2881 ttcgttttca acaagcgcgg cgcgatgccg cttactcaag aagaaagaat tatgacgttg
     2941 tctccttatt tgcaagaggt ggcgaagcgc cgcacttttg ccattatttc tcacccggac
     3001 gccggtaaga ctaccatcac cgagaaggtg ctgctgttcg gacaggccat tcagaccgcc
     3061 ggtacagtaa aaggccgtgg ttccaaccag cacgctaagt cggactggat ggagatggaa
     3121 aagcagcgtg ggatctccat tactacgtct gtgatgcagt ttccgtatca cgattgcctg
     3181 gttaacctgc tcgacacccc ggggcacgaa gacttctcgg aagataccta tcgtaccctg
     3241 acggcggtgg actgctgcct gatggttatc gacgccgcaa aaggtgttga agatcgtacc
     3301 cgtaagctga tggaagttac ccgtctgcgc gacacgccga tcctcacctt tatgaacaaa
     3361 cttgaccgtg atatccgcga cccgatggaa ttgctcgatg aagttgagaa cgagctgaaa
     3421 attggctgcg caccgatcac ctggccaatt ggctgcggca agctgtttaa aggcgtttac
     3481 cacctttata aagatgaaac ctatctctat cagagcggta aagggcacac cattcaggaa
     3541 gtccgcattg ttaaagggct gaataacccg gatctcgatg ctgcggttgg tgaagatctg
     3601 gcacagcagc tgcgtgacga actggaactg gtgaaaggcg cgtctaacga gttcgacaaa
     3661 gagctgttcc ttgcgggcga aatcactccg gtattcttcg gtactgcgct gggtaacttc
     3721 ggcgttgatc atatgctgga tggcctggtg gagtgggcgc ctgcgccgat gccgcgtcag
     3781 accgataccc gtaccgtaga agcgagcgaa gataaattta ccggcttcgt atttaaaatt
     3841 caggccaaca tggacccgaa acaccgcgac cgcgtggcgt ttatgcgtgt ggtgtccggt
     3901 aaatatgaaa aaggcatgaa gctgcgccag gtgcgcactg cgaaagatgt ggtgatctcc
     3961 gacgcgctga cctttatggc gggtgaccgt tcgcacgttg aagaagcgta tccgggcgat
     4021 atcctcggcc tgcacaacca cggcaccatt cagatcggcg acacctttac ccagggtgag
     4081 atgatgaagt tcaccggtat tccgaacttc gcaccagaac tgttccgtcg tatccgcctg
     4141 aaagatccgc tgaagcaaaa acagctgctc aaagggctgg tacagctttc cgaagagggc
     4201 gcggtgcagg tgttccgtcc aatctccaac aacgatctga tcgttggtgc ggttggtgtg
     4261 ctgcagtttg atgtggtggt agcgcgcctg aagagcgaat acaacgttga agcagtgtat
     4321 gagtcagtca acgttgccac tgcccgctgg gtagaatgtg cagacgcgaa gaaattcgaa
     4381 gagttcaagc gtaagaacga aagccaactg gcgcttgatg gcggcgataa cctcgcttac
     4441 atcgctacca gcatggtcaa cctgcgcctg gcacaggaac gttatccgga cgttcagttc
     4501 caccagaccc gcgagcatta attcttgcct ccagggcgcg gtagccgctg cgtcctgtca
     4561 atttcccttc cttattagcc gcttacggaa tgttcttaaa acattcactt ttgcttatgt
     4621 tttcgctgat atcccgagcg gtttcaaaat tgtgatctat atttaacaaa gtgatgacat
     4681 ttctgacggc gttaaatacc gttcaatgcg tagatatcag tatctaaagc cgtcgattgt
     4741 cattctaccg atattaataa ctgattcaga ggctgtaatg gtcgttattc atcactcatc
     4801 gcttttgtga tggcgaccat tgacttctgt agagggtgaa gtctctccct attcagcaat
     4861 gcaacctcgt gttgccaggc tcaaattacg agcaaacata caggaataaa tcgatgacta
     4921 tgacaagact gaagatttcg aaaactctgc tggctgtaat gttgacctct gccgtcgcga
     4981 ccggctctgc ctacgcggaa aacaacgcgc agactaccaa tgaaagcgca gggcaaaaag
     5041 tcgatagctc tatgaataaa gtcggtaatt tcatggatga cagcgccatc accgcgaaag
     5101 tgaaggcggc gctggtggat catgacaaca tcaagagcac cgatatctct gtcaaaaccg
     5161 atcaaaaagt cgtgaccctg agcggtttcg ttgaaagcca ggcccaggcc gaagaggcag
     5221 tgaaagtggc gaaaggtgtt gaaggggtga cctctgtcag cgacaaactg cacgttcgcg
     5281 acgctaaaga aggctctgtg aagggctacg cgggtgacac cgccaccacc agtgaaatca
     5341 aagccaaatt gctggcggac gatatcgtcc cttcccgtca tgtgaaagtt gaaaccaccg
     5401 atggcgtggt tcagctctct ggtatcgtag attctcaggc acaaagtgac cgtgctgaaa
     5461 gtatcgccaa agcggtagat ggtgtgaaaa gcgttaaaaa tgatctgaaa actaagtaat
     5521 tcgtcgtaat tcgtcctccc gaactttgtc gggaggcgcg atgtgcacca cactaaaaat
     5581 atcgcattga gtgattttca cgctcatatc aagcggtgac attaactatg gtaaaggaga
     5641 cgcttatgtt tcgttggggc atcatatttc tggttatcgc gttaatcgcc gccgcacttg
     5701 ggtttggtgg tctggccggt accgctgcag gcgcagctaa aattgtcttt gtcgtcggga
     5761 ttattctgtt cctggtgagt ttgttcatgg gccgaaaacg accctagatt tcagaccatt
     5821 aagaaaattt tatccaaagc cagtccagcg gactggcttt tgcggtttta gcgaataata
     5881 aattgcgtta ctttgtcatt ctttcacaac aaaaacagga aggcagaggt ggggcagcga
     5941 atacctgtaa cgcttggtaa tattgcgccg ttgtcgctaa ggccgttcca gcctggacga
     6001 atagctctgg tgtgcgaagg cggcggacag cgtggaattt tcacggctgg cgtgctggat
     6061 gagtttatgc gcgcgcagtt taatcctttc gatctttatc tcggcacatc tgccggggcg
     6121 cagaacctct cggcgtatat ctgcaatcag cccggttacg cgcgcaaagt catcatgcgc
     6181 tataccacaa aacgcgaatt tttcgatcca ttgcgctttg tccgtggagg aaatcttatc
     6241 gatctcgact ggctggtgga ggccaccgca agccagatgc cgttgcaaat ggataccgcc
     6301 gcgcggttgt ttgacagcgg caaatcgttt tatatgtgcg cctgtcgtca ggatgactac
     6361 gcgccgaatt actttttacc aaccaaacaa aactggctgg atgtgattcg cgcctccagt
     6421 gcgatacctg gcttttatcg tagcggagtg tcgctggaag gcattaacta cctggatggc
     6481 gggatcagtg atgcgattcc ggttaaagag gcggcaaggc agggcgctaa aacgttggtc
     6541 gtcatccgca ctgtgccgtc acaaatgtac tacacgccgc agtggttcaa acgcatggaa
     6601 cgctggctgg gtgacagtag cctgcagccg ctggtcaatc tggtgcagca tcatgaaacc
     6661 agctatcgtg acattcagca atttattgag aaaccaccgg gcaagctgcg gatattcgaa
     6721 atttatccgc cgaagccatt acatagtatc gcgcttggca gtcggattcc ggcgctgcgt
     6781 gaagactata aacttgggcg tttatgcggt cgttatttcc tcgccacggt tggcaagcta
     6841 ttaactgaaa aagcgccgct tacccgccat ctggtgccag tggtgacgcc ggaatcgatt
     6901 gtcattccgc ctgcgccagt cgccaacgat acgctggttg ccgaagtgag cgacgctccg
     6961 caggcgaacg acccgacatt taacaatgag gatctggctt gatttgccgt tttatcgaca
     7021 cccactgcca ttttgatttc ccgcctttta ttggcgatga agaggccagc ctgcaacgcg
     7081 cggcacaagc gggcgtaggc aagatcattg ttccggcaac cgaggcggaa aattttgccc
     7141 gcgtgcaggc gttagcggaa aattatcaac cgctgtatgc cgcattgggc ttgcatcctg
     7201 gtatgttgga aaaacatagc gatgtgtctc ttgatcagct acagcaggcg ctggaaaggc
     7261 gtccggcgaa ggtggtggcg gtgggggaga gcggtctgga tctcttcggc gacgatccgc
     7321 aatttgagag gcagcagtgg tttctcgacg aacaactgaa actggcgaaa cgctacgatc
     7381 tgccggtgat cctgcattca cggcgcacac acgacaaact ggcgatgcat cttaaacgcc
     7441 acgatttatc gcgcactggc gtggttcacg gtttttccgg cagcctgcaa caggccgagc
     7501 ggtttgtaca gctgggctac aaaatcggcg taggcggtac tatcacctat ccacgcgcca
     7561 gtaaaacccg cgttgtcatc gcaaaattac cgctggcatc gttgttgctg gaaaccgacg
     7621 cgccggatat gccgctcaac ggttttcagg ggcagcctaa ccgcccggaa caggctgcgc
     7681 gggtgtttga cgtgctttgc gagttgcgcc cggaaccaga agatgagatt gccgaagtgt
     7741 tgcttaataa cacgtatgcg gtgtttaacg ttcgtgggta gtttgccctt cgtaggcagc
     7801 atccggcagt cggcgcataa tgcctgatgc gacgcttaac gcgtcttatc atgcctacag
     7861 gtttgtgccg aaccgtaggc cggataaggc gttcacgccg catccggcag ttggcgcaca
     7921 atgcctgatg cgacgcttga cgcgtcttat caggcctaca agtctgtgcc gaaccgtagg
     7981 tcgtatccgg catgtcacaa atacagcgcc ggaaatatta accggctcac cccgcgctcc
     8041 cgtaacgcat cagccaacgg ctcaacgtct tccggcgtgg cgctcgccca gctttgcgcc
     8101 tcgccataca cgccgtgggc atgaaacgcg ttcaggcgta ccggaacatc gccgactccc
     8161 ttgataaacg ccgccagttc ttcgatgtgt tgcaaataat ccacttggcc agggattacc
     8221 agcaaacgca gttccgccag cttaccgcgc tctgccagca aacagatgct gcgcttaatc
     8281 tgctgattat cgcgtccggt taattgctga tgacattcgc tcccccacgc tttgagatcg
     8341 agcattgcgc cgtcgcacac cgggagcaat ttttcccagc cggtttcgct caacataccg
     8401 ttactgtcca ccaggcaggt gaggtggcgc agttgcggat cgtttttgat agcagtaaac
     8461 agcgccacta caaacggcag ttgggtcgtg gcttcgccgc cactcaccgt tatcccttcg
     8521 ataaacagca ctgctttgcg gacatggcta agcacctcat ccacgctcat ggattgcgcc
     8581 attggcgtgg catgttgcgg acacctcttc aggcaggtat cacattgctc gcagaccaca
     8641 gcgttccaca ccactttgcc gtcaacaatc tgcaacgcct gatgcggaca ctgtggcacg
     8701 cactccccac agtcattgca acgtcccatc gtccacggat tgtgacagtt tttgcagcgc
     8761 agattgcagc cctgcaaaaa cagagccaga cgactgcctg gcccgtcaac gcaggagaag
     8821 gggataatct tactgactaa agcgcatctg ctgttcatgg cttatcacgc gcggctggcg
     8881 ttccagaata cgagtgttgc gtgcggcttc ttcgcccagc caggtggtgt tggtgcgtga
     8941 accttcggcg cgatattttt ctaaatccga caaacgcacc atataaccgg taacgcgaac
     9001 cagatcgtta ccgctgacat tggcggtaaa ttcacgcatt ccggctttaa aggcaccgag
     9061 gcaaagctgt accaacgcct gcgggttacg tttgatggtt tcgtcgagcg tcagaatgtc
     9121 gctgatgcca gaataataat aagcatgatg cgggcgacag tttgcagatg ggtgatcgga
     9181 tctggctcat cgccatacgg caaacgcgcg cccggcgtgg tgccgatatc ggaactgatc
     9241 cccgactgtg cgtgtaacat ggcgcgtttt tgccagccat atttcacggg ggtattggcg
     9301 acaaactccg ccagctgtgc actgatgcga taacctactt catttgcggc ggcttcttta
     9361 ccgtagcgcg cggcaatccc ttctttttca cacagcaagt taaccgcttc cgccagccca
     9421 tacatgccaa acattggcac aaaacgttca gggttaatca gcccttcttt caccaggaag
     9481 ctattctcaa agaagtgtga ttgttgatag aggaattcac accgcgcatc gatgatggcg
     9541 atctgctgct ggcagtagtg cggtagagtg cgcgtaaaga agtcatccag cgattcgctg
     9601 cgctcggcaa tggctttcag gttaaggcgt accagcgtgc tgccaccacc cgccagcggc
     9661 agtgagttgt aacagctcac aatcccgtag cccccttttg tgaaaatttt atcatgcacc
     9721 ggaccgttgg cgatgtgcgg tttgctacat tcacagatgt tcttcgccac ttccagcagc
     9781 aggtcatcag gggtgatttc aggatcgtag ataaaggtca ggttcggtga aacctgcttc
     9841 aaatctgcgt ctgcacgtaa gatcgcgcgg gtaatgggcg aatcagacgg gccgatattg
     9901 gcgtgcataa aggcgtctgg cagggttctg tcgaggtaac gccagaaacg ttttattcga
     9961 acatcgatct cgtcttgtgt tagaattata acatacggtt gcaacaacgc atccagttgc
    10021 cccaggtaga ccggcatcga tgtgaccgac ggtacgtggt ggtaaagaat ggtcagcaga
    10081 gagagtgcgt catcaagatc tttcgcgcct tccagctcca gccattcgga accgttcgcc
    10141 agaaaacggg cgtaatcggg taagacatag cgcggtttgt acggcgcatg accttcaaac
    10201 atatcgcaga ttacaccttc atccagcgcg cggcgggctt cggcaggaag ctgtgggtaa
    10261 ggcagattgt tttctgcttc cagtgccaga aaatggcgct tctgctccgg gctaagcact
    10321 gggctggtga caatttgctg gcaacgttgt tgcagtgcat tttcatgaga agtgggcatc
    10381 ttcttttcct tttatgccga aggtgatgcg ccattgtaag aagtttcgtg atgttcactt
    10441 tgatcctgat gcgtttgcca ccactgacgc attcatttga aagtgaatta tttgaaccag
    10501 atcgcattat agtgatgcaa acttgtaagt agatttcctt aattgtgatg tatatcgaag
    10561 tgtgttgcgg agtagatgtt agaatactaa caaactcgca aggtgaattt tattggcgac
    10621 aagccaggag aatgaaatga ctgatctgaa agcaagcagc ctgcgtgcac tgaaattgat
    10681 ggacctgacc accctgaatg acgacgacac cgacgagaaa gtaattgctc tgtgtcatca
    10741 ggccaaaact ccggtcggca ataccgccgc tatctgtatc tatcctcgct ttatcccgat
    10801 tgctcgcaaa acactgaaag agcagggcac cccggaaatc cgtattgcta cggtaaccaa
    10861 cttcccacac ggtaacgacg acatcgaaat cgcgctggca gaaacccgtg cggcaatcgc
    10921 ctacggtgct gatgaagttg acgttgtgtt cccgtaccgc gcgctgatgg cgggtaacga
    10981 gcaggttggt tttgacctgg tgaaagcctg taaagaggct tgcgcggcag cgaatgtact
    11041 gctgaaagtg atcatcgaaa ccggcgaact gaaagacgaa gcgctgatcc gtaaagcgtc
    11101 tgaaatctcc atcaaagcgg gtgcggactt catcaaaacc tctaccggta aagtggctgt
    11161 gaacgcgacg ccggaaagcg cgcgcatcat gatggaagtg atccgtgata tgggcgtaga
    11221 aaaaaccgtt ggtttcaaac cggcgggcgg cgtgcgtact gcggaagatg cgcagaaata
    11281 tctcgccatc gcagatgaac tgttcggtgc tgactgggct gatgcgcgtc actaccgctt
    11341 tggtgcttcc agcctgctgg caagcctgct gaaagcgctg ggtcacggcg acggtaagag
    11401 cgccagcagc tactaagtaa gatgctttac gcctgatgcg ctgcgcttat caggcctacg
    11461 agacgtatct acccgtaggc cggataaggc gtagacgcat ccggcaaaag ccgcctcata
    11521 ctcttttcct cgggaggtta ccttgtttct cgcacaagaa attattcgta aaaaacgtga
    11581 tggtcatgcg ctgagcgatg aagaaattcg tttctttatc aacggtattc gcgacaacac
    11641 tatctccgaa gggcagattg ccgccctcgc gatgaccatt ttcttccacg atatgacaat
    11701 gcctgagcgt gtctcgctga ccatggcgat gcgagattca ggaaccgttc tcgactggaa
    11761 aagcctgcat ctgaatggcc cgattgttga taaacactcc accggtggcg tcggcgatgt
    11821 gacttcgctg atgttggggc cgatggtcgc agcctgcggc ggctatattc cgatgatctc
    11881 tggtcgcggc ctcggtcata ctggcggtac gctcgacaaa ctggaatcca tccctggctt
    11941 cgacattttc ccggatgaca accgtttccg cgaaattatt aaagacgtcg gcgtggcgat
    12001 tatcggtcag accagctcac tggctccggc ggataaacgt ttctacgcga cccgtgatat
    12061 taccgcaacc gtggactcca tcccgctgat cactgcctcg atcctggcga agaaactggc
    12121 ggaaggtctg gacgcgctgg tgatggacgt gaaagtgggt agcggcgcgt ttatgccgac
    12181 ctacgaactc tctgaagccc ttgccgaagc gattgttggc gtggctaacg gcgcaggcgt
    12241 gcgcaccacc gcactgctca ccgacatgaa tcaggtgctg gcctccagtg ccggtaacgc
    12301 ggttgaagta cgtgaagcgg tgcagttcct gacgggtgaa tatcgtaacc cgcgtctgtt
    12361 tgatgtcacg atggcgctgt gtgtggagat gcttatctcc ggcaaactgg cgaaagatga
    12421 cgccgaagcg cgcgcgaaat tgcaggcggt gctggacaac ggtaaagcgg cagaagtctt
    12481 tggtcgtatg gtagcggcac aaaaaggccc gaccgacttc gttgagaact acgcgaagta
    12541 tctgccgaca gcgatgctga cgaaagcagt ctatgctgat accgaaggtt ttgtcagtga
    12601 aatggatacc cgcgcgctgg ggatggcagt ggttgcaatg ggcggcggac gccgtcaggc
    12661 atctgacacc atcgattaca gcgtcggctt tactgatatg gcacgtctgg gcgaccaggt
    12721 agacggccag cgtccgctgg cggttatcca cgcgaaagac gaaaacagct ggcaggaagc
    12781 ggcgaaagcg gtgaaagcgg caattaaact tgccgataaa gcaccggaaa gcacaccaac
    12841 tgtctatcgc cgtatcagcg aataacggta tactgatctg atcatttaaa tttgaagcac
    12901 tgagtacgga gaacatatga aacgtgcatt tattatggtg ctggactcat tcggcatcgg
    12961 cgctacagaa gatgcagaac gctttggtga cgtcggggct gacaccctgg gtcatatcgc
    13021 agaagcttgt gccaaaggcg aagctgataa cggtcgtaaa ggcccgctca atctgccaaa
    13081 tctgacccgt ctggggctgg cgaaagcaca cgaaggttct accggtttca ttccggcggg
    13141 aatggacggc aacgctgaag ttatcggcgc gtacgcatgg gcgcacgaaa tgtcatccgg
    13201 taaagatacc ccgtctggtc actgggaaat tgccggtgtc ccggttctgt ttgagtgggg
    13261 atatttctcc gatcacgaaa acagcttccc gcaagagctg ctggataaac tggtcgaacg
    13321 cgctaatctg ccgggttacc tcggtaactg ccactcttcc ggtacggtca ttctggatca
    13381 actgggcgaa gagcacatga aaaccggcaa gccgattttc tatacctccg ctgactccgt
    13441 gttccagatt gcctgccatg aagaaacttt cggtctggat aaactctacg aactgtgcga
    13501 aatcgcccgt gaagagctga ccaacggcgg ctacaatatc ggtcgtgtta tcgctcgtcc
    13561 gtttatcggc gacaaagccg gtaacttcca gcgtaccggt aaccgtcacg acctggctgt
    13621 tgagccgcca gcaccgaccg tgctgcagaa actggttgat gaaaaacacg gccaggtggt
    13681 ttcggtcggt aaaattgcag acatctacgc caactgcggt atcaccaaaa aagtgaaagc
    13741 gactggcctg gacgcgctgt ttgacgccac catcaaagag atgaaagaag cgggtgataa
    13801 caccatcgtc ttcaccaact tcgttgactt cgactcttcc tggggccacc gtcgcgacgt
    13861 cgccggttat gctgcgggtc tggaactgtt cgaccgccgt ctgccggagc tgatgtctct
    13921 gctgcgcgat gacgacatcc tgatcctcac cgctgaccac ggctgtgatc cgacctggac
    13981 cggtactgac cacacgcgtg aacacattcc ggtactggtt tacggcccga aagtaaaacc
    14041 gggctcactg ggtcaccgtg aaaccttcgc ggatatcggc cagactctgg caaaatattt
    14101 tggtacttct gatatggaat atggcaaagc catgttctga tggatttggg cggagcgttg
    14161 actccgcctt tgttatgtca caaaaaggat aaaacaatgg ctaccccaca cattaatgca
    14221 gaaatgggcg atttcgctga cgtagttttg atgccaggcg acccgctgcg tgcgaagtat
    14281 attgctgaaa ctttccttga agatgcccgt gaagtgaaca acgttcgcgg tatgctgggc
    14341 ttcaccggta cttacaaagg ccgcaaaatt tccgtaatgg gtcacggtat gggtatcccg
    14401 tcctgctcca tctacaccaa agaactgatc accgatttcg gcgtgaagaa aattatccgc
    14461 gtgggttcct gtggcgcagt tctgccgcac gtaaaactgc gcgacgtcgt tatcggtatg
    14521 ggtgcctgca ccgattccaa agttaaccgc atccgtttta aagaccatga ctttgccgct
    14581 atcgctgact tcgacatggt gcgtaacgca gtagatgcag ctaaagcact gggcattgat
    14641 gctcgcgttg gtaacctgtt ctccgctgac ctgttctact ctccggacgg cgaaatgttc
    14701 gacgtgatgg aaaaatacgg cattctcggc gtggaaatgg aagcggctgg tatctacggc
    14761 gtggctgcag agtttggcgc gaaagccctg accatctgca ccgtgtctga ccacatccgc
    14821 actcacgagc aaaccactgc cgctgagcgt cagaccacct tcaacgacat gatcaaaatc
    14881 gcactggaat ccgttctgct gggcgataaa gagtaattgt gtttcgctga aaggcgattg
    14941 tcgtgttaag ccggagtggg ggactgctcc ggctttttag tatct
//
LOCUS       DAZKFZ010000103        14941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000103 DAZKFZ010000000
VERSION     DAZKFZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            129..371
                     /gene="yjbE"
                     /locus_tag="ACHOQW_002863"
     CDS             129..371
                     /gene="yjbE"
                     /locus_tag="ACHOQW_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP9573913.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            485..1123
                     /gene="yjbF"
                     /locus_tag="ACHOQW_002864"
     CDS             485..1123
                     /gene="yjbF"
                     /locus_tag="ACHOQW_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP9573914.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1120..1857
                     /gene="yjbG"
                     /locus_tag="ACHOQW_002865"
     CDS             1120..1857
                     /gene="yjbG"
                     /locus_tag="ACHOQW_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9573915.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1857..3953
                     /gene="yjbH"
                     /locus_tag="ACHOQW_002866"
     CDS             1857..3953
                     /gene="yjbH"
                     /locus_tag="ACHOQW_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9573916.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4000..4278)
                     /locus_tag="ACHOQW_002867"
     CDS             complement(4000..4278)
                     /locus_tag="ACHOQW_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573917.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4279..4422
                     /locus_tag="ACHOQW_002868"
     CDS             4279..4422
                     /locus_tag="ACHOQW_002868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573918.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4548..4958
                     /gene="psiE"
                     /locus_tag="ACHOQW_002869"
     CDS             4548..4958
                     /gene="psiE"
                     /locus_tag="ACHOQW_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP9573919.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5002..6477)
                     /gene="xylE"
                     /locus_tag="ACHOQW_002870"
     CDS             complement(5002..6477)
                     /gene="xylE"
                     /locus_tag="ACHOQW_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP9573920.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6849..7739)
                     /gene="malG"
                     /locus_tag="ACHOQW_002871"
     CDS             complement(6849..7739)
                     /gene="malG"
                     /locus_tag="ACHOQW_002871"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP9573921.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7754..9298)
                     /gene="malF"
                     /locus_tag="ACHOQW_002872"
     CDS             complement(7754..9298)
                     /gene="malF"
                     /locus_tag="ACHOQW_002872"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP9573922.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9452..10642)
                     /gene="malE"
                     /locus_tag="ACHOQW_002873"
     CDS             complement(9452..10642)
                     /gene="malE"
                     /locus_tag="ACHOQW_002873"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP9573923.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11007..12122
                     /gene="malK"
                     /locus_tag="ACHOQW_002874"
     CDS             11007..12122
                     /gene="malK"
                     /locus_tag="ACHOQW_002874"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP9573924.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12194..13534
                     /gene="lamB"
                     /locus_tag="ACHOQW_002875"
     CDS             12194..13534
                     /gene="lamB"
                     /locus_tag="ACHOQW_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP9573925.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13685..14605
                     /gene="malM"
                     /locus_tag="ACHOQW_002876"
     CDS             13685..14605
                     /gene="malM"
                     /locus_tag="ACHOQW_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP9573926.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14834..>14941
                     /locus_tag="ACHOQW_002877"
     CDS             14834..>14941
                     /locus_tag="ACHOQW_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573927.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNG"
BASE COUNT         3686 a         3840 c         3939 g         3476 t
ORIGIN      
        1 ttccaattat cctaaaaccc catctctaat tccccgcgcc gtaattcgca tgctttagtt
       61 gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt caatgaaggg
      121 aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt gcggcgactt
      181 ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg gtcggcagca acgtcggttt
      241 cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg ggtgtcgcgc
      301 tcgcggcaac cggtggcggt gatggttcta ataccgggac caccacaacc acgaccacca
      361 gtacccagta ataaagtatg tatccccaaa ataattcgag tcattgcatc tgtggctaga
      421 agtatgaagg gatttaccat aaccacactc cggtgtggtt attctgcccc tctggagaag
      481 agtcgtgaag cgacctgcat tcattcttat ctgcctgcta ttacaggcct gttcagccac
      541 gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag gcgtacagct
      601 gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc ttaatggtgg
      661 gccgcagttg tttgtggtgc tggccttcgc tgaagacgga caacaaaaat gggtcactca
      721 ggatcaggcc actctcgtca cacaacatgg tcgtctggtg aagactttgc ttggcggcga
      781 caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg cacaaatcat
      841 tgatggcgca acctggatgc gcacgatggg ctggaccgag taccagcagg tacgctacgc
      901 caccgcacgc tcagtcttca aatgggatgg caccggtacc gttaaggtcg gcagcgatga
      961 aaccgcggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct ggcataaccg
     1021 ctattggatc gacagcggag ggcaaattcg ccagtcggaa cagtatctcg gcgcggatta
     1081 tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca aactattgtc
     1141 gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt caaggtgttc
     1201 agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat cgatctggta
     1261 ggccaaccgc ggctggcaaa cagctggtgg cctggtgcgg tgattagcga agagctggca
     1321 acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga acagggcgca
     1381 gattccagcg ccgacgatgc cgctgcgatt aacgccttac gccagcaaat tcaggcgttg
     1441 aaggtgacgg gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt tgctgaacgc
     1501 gctaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccacc gtccacggtc
     1561 acgttgttcg ggcttatcag ccgtcctggc aatcagccat tcactcccgg tcgcgacgtg
     1621 gcgagctatc tctccgggca aagtctgctt agcggtgcgg atcgtagcta cgcgtgggtg
     1681 gtttacccgg acggacgcac acaaaaagcg ccggtggctt actggaacaa gcgtcacgta
     1741 gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg actccgtctg gagtgagatg
     1801 cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc tcaataatga
     1861 aaaaaagaca tctgcttagc ttactggcgc tgggcattag cacagcttgc tacggcgaaa
     1921 catatcctgc gcccattggt ccgtcgcagt cggatttcgg tggcgtagga ttattacaaa
     1981 cgcccacggc tcgcatggcg cgggaagggg agttgagtct gaactatcgc gataacgatc
     2041 agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca acgctgcgct
     2101 acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc gatcaaacgt
     2161 ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac tggctgccgc
     2221 aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gtttgatgcg gaatatcttg
     2281 ttgccagcaa agcctggggg ccgttcgatt ttacgcttgg cctgggctgg ggatatttgg
     2341 gcacaagcgg taatgtgaaa aatccgctct gttcagccag tgataaatat tgctatcgcg
     2401 ataacagcta caaacaggcg ggatctatcg acggtagcca gatgttccac ggtcctgcct
     2461 cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg aaactggagt
     2521 atgaaggcaa taattatcag caggattttg ccggtaagct ggagcaaaaa agtaagttta
     2581 acgtcggggc gatttatcgc gttaccgatt gggccgacgt taaccttagc tatgaacgtg
     2641 ccaacacctt tatgtttggc gtcacgctgc gcaccaactt taacgatctg cgcccgtctt
     2701 acaacgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt ttgcagcatt
     2761 cggtggtggc gaatcagtta acgctgttga aatacaatgc cggacttgcc gatccgcaga
     2821 tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat cgtgattcgc
     2881 gcgaagggat cgttcgtgct aatcggatcg tgatgaatga tctgccggat gggatcaaga
     2941 cgatccgcgt tacggaaaat cgccttaaca tgccgcaggt gacgacggaa acggatgtcg
     3001 ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgaag ctggcgcaaa
     3061 aacgcgtcga gccagtggtt ccgaagtcca ccgagcaggg ctggtatatc gacaaatcgc
     3121 gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg gaaaactttt
     3181 acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac catctgctga
     3241 ccaccggcag cctgtttgca aatcttgcca acaactacga caagtttaac tacaccaatc
     3301 cgccgcagga ctcgcactta ccgcgcgtgc gtacccatgt gcgcgagtat gtgcagaacg
     3361 atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac ggcttctacg
     3421 gtcaggtcta cggaggttat ctcgaaacca tgtttggcgg tgcgggggca gaagtgttgt
     3481 atcgcccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt aaacagcgcg
     3541 actggcgtag tgcaaaggat atgatgaaat tcaccgacta cagcgtgaaa accggacatc
     3601 tgaccgccta ctggacgcca tctttcgctc aggatgtgtt agttaaagcc agcgtcgggc
     3661 agtatctggc aggggataaa ggcggcacgc tggagatcgc taaacgcttt gacagcggcg
     3721 tggtggtggg cggctatgcc acgatcacca acgtttcgaa agaggagtac ggcgaagggg
     3781 acttcaccaa aggcgtgtat gtctctgtac cgttggatct cttctcgtct ggcccgacac
     3841 gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcag caacttgggc
     3901 gtaagttcca gttgtatgac atgaccagcg accgtagcgt caatttccgc taagtcatgg
     3961 gaaaggtgcc agttttcgca atcactggca cctttattct taatggcctg ttgagccatc
     4021 aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac attctgtctt
     4081 cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca tcattgggct
     4141 ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac cagcataagc
     4201 aggaagagca gagactgata acatcaaagc agcaaaataa ggcttcattt ttaccacctt
     4261 tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg cattgctggt
     4321 ggctgggaat cacctgaatg ggtgattttt gaattaccgg ctttggtgcg gtttgtcttg
     4381 ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc ggctttggtg
     4441 gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga tctccgtcac
     4501 atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg acgtcactct
     4561 ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat cttggcctgc
     4621 tgtgcctcgg ccttattttg gttgtcttcc tcggcaaaga aacggtgcat ctggctgatg
     4681 tgctgttcgc gccagaacaa gccagcaaat atgagctggt agaaggactg gtggtttact
     4741 ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt tttcacttcc
     4801 cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc atcgtcgatc
     4861 ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg gtggtcaccc
     4921 tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agacagcacg ccgaactggc
     4981 gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct tcgtttccgg
     5041 ttcccagagg gcttccagct cctcaagggt tttacctttg gtttccggga caaatttcca
     5101 cataaacagt gctgccagaa cgcccataca accgtaaatc cagtaggaga aaccgttgtg
     5161 gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg agacgaagta
     5221 gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttacctcgaa tagcattcgg
     5281 gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa aggcggcaac
     5341 atagaacagc atcgacagta gcgccacaat acccggtgcc tgagtgtaaa acgcggtacc
     5401 gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg gcttacgacc
     5461 aaatttatcc accgtcataa ttgccagaac ggtgaaggtg aggttgataa ctccgacaat
     5521 aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca cttccggcgc
     5581 gtagtacagc accacattga tgccgacaaa ttgctggaag atggagagca taacgccgat
     5641 gacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc catgatccag
     5701 ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa ttttgcgcag
     5761 gataccttcc gcctgttctt gcttgccgcg tgacatcagc cagcgaggac tttctggcac
     5821 ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa acatataacg
     5881 ccagccgtca gtattcagcc agctggcatc accggaacgc gcaataaaat agtttacgca
     5941 gtaaactaaa agctgcccga aaataatcgc aaactggtta aatgaaacca gtttcccgcg
     6001 aatatgagca ggagcaagtt ccgcaatata cattggtgaa agcattgagg ctaaaccaac
     6061 gccaataccg ccaataatac gataaataac aaattccggg acataacctg ccagataaac
     6121 aggcactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg cagaacctac
     6181 accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacgac cgaagcggtt
     6241 actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc tggccacgca
     6301 aaaccctaac agggagttgg cagcggattc acttaagttt tgtggagcaa caaagacggt
     6361 attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata ataaaccacc
     6421 taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg tattcattca
     6481 gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat tgtcaggcag
     6541 ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt gatcttaatt
     6601 gtgataatta tccaaaattg ataaaaacaa tactattgcc gtgactcaga gcacgaaaga
     6661 gaattatcgt aagtgggaaa acaaataacg taaaaataat aagctctatg atggaaatat
     6721 taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt ggagcgccga
     6781 ataacagtca caagttggga taacgtaagt tgagggtgca gcggcataac attggcagaa
     6841 caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca gcgttgagcc
     6901 agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc aaagtcaccc
     6961 cacaggtagt tttgcggatt gaggtattgc tgcatcccca cggccagggt gtagctgttt
     7021 acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat aaacgacagg
     7081 atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg gaaggcctgc
     7141 cacggtgtcg caccatccag cgcagccgct tcttccagcg aactgtcgat ggtttcgaaa
     7201 tagcctttga tggtccagac gtgcagcgca atcccaccca gatacgcgaa aattacgccg
     7261 ccgtgagtat tcaggccaat gaatggaatg tactcaccca gacgatcaaa caacgcatac
     7321 aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc tttcagcagc
     7381 gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt ggagagcgcc
     7441 acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccacagcca cagcagcacc
     7501 gggaatggcg gtggcgtaat gcgaccatca gcctgttcca cgctaaaacc taatgccagt
     7561 ttccagtgat cccaggagat ttgctccggg atcaggctgc cggtcgcaaa gttcccctgg
     7621 cgcagtgaga tagcgacgac catcagcagc gggaacataa tcgccgcgat aaaaagtagc
     7681 agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac cattgccatt
     7741 tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact atcgccagcg
     7801 cacccaccag caggaagatc agcgtggcaa ttgctgctgc cagaccgaag tcctgacccc
     7861 cgccgccttc aaaagcgatg cggtaggtgt agttaacaag caggtcggta taaccggctg
     7921 gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagttgaatc agcacgaagt
     7981 tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta atcagcagcg
     8041 gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct gaggcttcat
     8101 acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg tacggatagc
     8161 ccagccaggt attgacgata atcagcatcg ttcgggcggt ggtcggatcg ctgaaccagg
     8221 cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ctcaccgaag ctctggttaa
     8281 acaacccttt gaaaatcaag attgaaatga acgatggcac cgcgtagggc agaatcagca
     8341 ggacgcgata gaccgctttg ccgcgcaacg cttcccactg caccagacac gccagaacca
     8401 tgccgactgc caccgttaaa aagacagtga tcagcgagaa caccacggtc cagacgaaaa
     8461 tggcgaggaa cggtttctga atgccttcgt cggtgaagac gcgagtaaag tttttccagc
     8521 cggtggtcac ggtgtaaccg gggcttagct tttcatcacc ccagttgccg tcggcggtaa
     8581 tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc tgattattcg
     8641 tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac tggcgcaggg
     8701 agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc agcgcctgac
     8761 gattctgggt aatcacgcgt agatttgcgc gttcgccttc gggctgggcg gtcgtttctt
     8821 tcagttgcag tttttgctct ctgccaaatt taaaagcgtc ggagaggtaa tttttgccgg
     8881 tttcgccgtc gctgagcgcc agttgccact catcgcccgc cgggtaaaga ccaaagttat
     8941 aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgtgcacgt tcaaaagtca
     9001 gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc agagggaaga
     9061 ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg taggctttac
     9121 gattggcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg gcgaacaggt
     9181 attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg agcagaccta
     9241 acactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg acatccatgg
     9301 ggttcttcct cattccagga cggataaggc tttcatgcct tatccgacaa caactgcctg
     9361 atgcgacgct gacgcgtctt atcaggccta catacgtttc ggttttgtag gccggacaag
     9421 gcgttcacgc cgcatccggc atttcacagc attacttggt gatacgagtc tgcgcgtctt
     9481 tcagggcttc atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg
     9541 cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc tgggcgtttt
     9601 ccatggtggc tgcaatacgt ggatctttcg ccaactcttc ctcgtaagac ttcagcgcta
     9661 cggcacccag cggtttgtct ttattaaccg cttccagacc ttcgtcagtc agcagatagt
     9721 tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata ccggcgctca
     9781 gcacgccaac gaacggttta gatggttgac ccttgaaggt cggcagtacc gttacaccat
     9841 aattcacttt gctggtgtcg atgttggacc atgcccacgg gccgttgata gtcatcgctg
     9901 tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt
     9961 tgtttttaat caggtcaacc aggaaggtca gacccgcttt cgcgccagcg ttatccacgc
    10021 ccacgtcttt aatgtcgtac ttgccgtttt catacttgaa cgcataaccc ccgtcagcag
    10081 caatcagcgg ccaggtgaag tacggttctt gcaggttgaa catcagcgcg ctcttacctt
    10141 tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggc gggttcggta
    10201 gcagatcttt gttataaatc agcgataacg cttcaacagc gatcgggtaa gcaatcagct
    10261 tgccgttgta acgtacggca tcccaggtaa acggatacag cttgtcctgg aacgctttgt
    10321 ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaagcgg tcgtgcgccc
    10381 agaagataat gtcagggcca tcgccagttg ccgcaacctg agggaatttc tcttccagtt
    10441 tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc ttaccgactt
    10501 cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta ccttcttcga
    10561 ttttggcgag agccgaggcg gaaaacatca tcgtcgttaa tgcggataat gcgaggatgc
    10621 gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct cgtgaaaaca
    10681 cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt gcccctacgc
    10741 cccaccgttg ctttgtgtga tctctgttac agaattggca gtaatgtgga gatgcgcaca
    10801 taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac ctcggtttag
    10861 ttcacagaag ccgcgttctc atcctcccgc ctcctccccc ataaaaaagc tagggggtgg
    10921 aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt gctgtcgatg
    10981 acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat gtaacgaaag
    11041 cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa ggtgaattcg
    11101 tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg attgccgggc
    11161 ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat gacactccgc
    11221 cagcagaacg tggcgttggt atggtgtttc agtcctacgc gctctatccc cacctgtcag
    11281 tagcagaaaa catgtcattt ggcctgaaac tggcaggcgc aaaaaaagag gtgattaacc
    11341 aacgcgtcaa ccaggtggcg gaagtgctac aactggcgca tttgctggat cgcaaaccga
    11401 aagcgctctc cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg gtggccgagc
    11461 caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg cgtgtgcaaa
    11521 tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt tacgtcaccc
    11581 acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac gccggtcgcg
    11641 tggcgcaggt tgggaaaccg ctggagctgt accactatcc ggcagaccgt tttgtcgccg
    11701 gatttatcgg ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc accgcaatcg
    11761 atcaagtgca ggtggagctg ccgatgccaa atcgtcagca agtctggctg ccagttgaaa
    11821 gccgtgatgt ccaggttgga gccaatatgt cgctgggtat tcgcccggaa catctactgc
    11881 cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag caactcggca
    11941 acgaaaccca aatccatatc cagatccctt ccattcgtca aaacctggtg taccgccaga
    12001 acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg ccagagcgtt
    12061 gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag ccgggcgttt
    12121 aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga aaagcaatga
    12181 ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg ccgtcgcagc
    12241 gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac gttccggtat
    12301 tggctggaca ggtagcggcg gtgaacaaca gtgtttccag actaccggtg ctcaaagtaa
    12361 ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc aggaagtgtg
    12421 gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg tcgcgcaaca
    12481 gaatgactgg gaagctaccg acccggcctt ccgtgaagca aacgtgcagg gtaaaaacct
    12541 gatcgaatgg ctgccaggct ccaccatctg ggcaggtaag cgcttctacc aacgtcatga
    12601 cgttcatatg atcgacttct actactggga tatttctggt cctggtgccg gtctggaaaa
    12661 catcgatgtt ggcttcggta aactctctct ggcagcaacc cgctcctctg aagcaggtgg
    12721 ttcttcctct ttcgccagca acaatattta tgactatacc aacgaaaccg cgaacgacgt
    12781 tttcgatgtg cgtttagcgc agatggaagt caacccgggc ggcacattag aactgggtgt
    12841 cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg catcgaaaga
    12901 cggctggtta ttcactgctg aacatactca gagtgtcctg aagggcttta acaagtttgt
    12961 tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc agggttctgg
    13021 cgttgcattt gataacgaaa aatttgccta caatatcaac aacaacggtc acatgctgcg
    13081 tatcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt acgtgggtat
    13141 gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg tcggtattcg
    13201 cccgatgtac aagtggacgc caatcatgag caccgtgatg gaaatcggct acgacaacgt
    13261 cgaatcccag cgcaccggcg acaagaacaa tcagtacaaa attaccctcg cacaacaatg
    13321 gcaggctggc gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa cctacgccaa
    13381 gtgggatgag aaatggggtt acgactacaa cggcgatagc aaggttaacc cgaactacgg
    13441 caaagccgtt cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca gcgacgagtg
    13501 gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc ggataaggcg
    13561 tttacgccgc atccggcata aaacaggttg tcattatctg aaaggggcga aagcccctct
    13621 gattatcggg tttagcgcgc tattgcctgg ctaccgctga gctccagatt ttgaggtgaa
    13681 aacaatgaaa atgaataaaa gtctcatcgc cctctgttta tcagcagggt tactggcaag
    13741 cgcgcctgga atcagccttg ccgatgttaa ctacgtaccg caaaacacca gcgacgcgcc
    13801 agccattcca tctgccgcgc tgcaacaact cacctggaca ccggtcgatc aatctaaaac
    13861 ccagaccacc caactggcga ccggcggcca acaactgaac gttcccggca tcagtggtcc
    13921 ggttgctgcg tacagcgtcc cggcaaacat tggcgaactg accctgacgc tgaccagcga
    13981 agtgaacaaa caaaccagcg tttttgcgcc gaacgtgctg attcttgatc agaacatgac
    14041 cccatcagcc ttcttcccca gcagttattt cacctaccag gaaccaggcg tgatgagtgc
    14101 agatcggctg gaaggcgtta tgcgcctgac accggcgttg gggcagcaaa aactttatgt
    14161 tctggtcttt accacggaaa aagatctcca gcagacgacc caactgctcg acccggctaa
    14221 agcctatgcc aaaggcgttg gtaactcgat cccggatatt cccgatccgg ttgctcgtca
    14281 taccaccgat ggcttactga aactgaaagt gaaaacgaac tccagctcca gcgtgttggt
    14341 cgggccttta tttggttctt ccgctccagc tccggttacg gtaggcaaca cggcggcacc
    14401 agctgtggct gcacccgctc cggtgccggt gaagaaaagc gagccgatgc tcaacgacac
    14461 ggaaagttat tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg ttgataaggc
    14521 gttaaaactg cttgatgaag ctgaacgcct gggatcgaca tctgcccgtt ccacctttat
    14581 cagcagtgta aaaggcaagg ggtaattacg ccccacagtg ctgattttgc aacaactggt
    14641 gcgtctcctg gcgcaccttt ttttatgctt cattccaggg atatgagcga ttttttatag
    14701 taactcactt cttcttcact aagaatatcc attatctcaa tgcattatca gagattcttt
    14761 tcctttcgcc ggtagtgtct ggacgctcag gctacttttt ccagttattt catttctgta
    14821 atgcagaggt tttatgttag gtcatatctc aaagtttgac ggcaataact ctttgataaa
    14881 acatggtgtg gtgcaaggaa ataatatagt agattttgat ttactacgta attttaatgg
    14941 g
//
LOCUS       DAZKFZ010000104        14922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000104 DAZKFZ010000000
VERSION     DAZKFZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            78..347
                     /locus_tag="ACHOQW_002878"
     CDS             78..347
                     /locus_tag="ACHOQW_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9573928.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHOQW_002879"
     CDS             316..1434
                     /locus_tag="ACHOQW_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9573929.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1601..2395
                     /gene="potC"
                     /locus_tag="ACHOQW_002880"
     CDS             1601..2395
                     /gene="potC"
                     /locus_tag="ACHOQW_002880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP9573930.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2392..3438
                     /gene="potD"
                     /locus_tag="ACHOQW_002881"
     CDS             2392..3438
                     /gene="potD"
                     /locus_tag="ACHOQW_002881"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP9573931.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHOQW_002882"
     CDS             complement(3594..4415)
                     /gene="cobB"
                     /locus_tag="ACHOQW_002882"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP9573932.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHOQW_002883"
     CDS             complement(4431..5342)
                     /gene="nagK"
                     /locus_tag="ACHOQW_002883"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP9573933.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHOQW_002884"
     CDS             complement(5371..6615)
                     /gene="lolE"
                     /locus_tag="ACHOQW_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP9573934.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHOQW_002885"
     CDS             complement(6615..7316)
                     /gene="lolD"
                     /locus_tag="ACHOQW_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP9573935.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHOQW_002886"
     CDS             complement(7309..8508)
                     /gene="lolC"
                     /locus_tag="ACHOQW_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP9573936.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHOQW_002887"
     CDS             8770..9843
                     /gene="ycfT"
                     /locus_tag="ACHOQW_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9573937.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            9971..13417
                     /gene="mfd"
                     /locus_tag="ACHOQW_002888"
     CDS             9971..13417
                     /gene="mfd"
                     /locus_tag="ACHOQW_002888"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9573938.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDISTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHOQW_002889"
     CDS             13561..14523
                     /gene="ldtC"
                     /locus_tag="ACHOQW_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP9573939.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTLPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHOQW_002890"
                     /gene_synonym="ycfR"
     CDS             complement(14606..14863)
                     /gene="bhsA"
                     /locus_tag="ACHOQW_002890"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP9573940.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         3724 a         3877 c         3809 g         3512 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgaa tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaactcga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagtac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
      901 accatggaag actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctggggta
     1261 attccaccag caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg
     1321 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     1381 accgatcgct ataacgatac tcgaggtcag gaatgggtta agttggttat ttgacaaaca
     1441 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     1501 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     1561 tgaaagtgtc ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg
     1621 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     1681 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     1741 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     1801 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     1861 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     1921 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     1981 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     2041 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     2101 cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc
     2161 aggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtggtttctt cgttcgtcac
     2221 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     2281 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     2341 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     2401 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     2461 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     2521 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     2581 accatgtatg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     2641 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     2701 ttaacgaact tcagcaatct cgatccagac atgctcaata agccgtttga cccgaataac
     2761 gattactcca ttccgtatat ctggggtgcg acggcgattg gcgttaacgg tgatgcggtg
     2821 gatccgaaat ctgtcaccag ctgggccgat ttgtggaagc ctgagtacaa aggcagcctg
     2881 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tacgtaagct gggctactcc
     2941 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     3001 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     3061 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     3121 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     3181 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     3241 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     3301 ttaagtccag aagtggcgaa cgataagacg ctctccccgg atgctgaaac cattaaaaat
     3361 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     3421 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta
     3481 gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt
     3541 gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     3601 cccttcagca acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc
     3661 tcggcaaatt cattaccgac ctgacttggt tcaagattca gctccacggt gtgcgcgcca
     3721 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     3781 gcaatgaaaa tatcggccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     3841 tcgccaaacc atactacgtg tgggcgcaag ggggccggga actggcagca atggcattta
     3901 tcttctgggg taacgtctcc ggtccagtcg agaacctgac cactttgtga acaacgaact
     3961 ttcagcagtt ccccatgcat atgaatcaca ttggtattac ctgcgcgttc atgcaggttg
     4021 tctatattct gcgtcaccag caaaaagcga tcgccgagag catcttgcag tttagccagc
     4081 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
     4141 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     4201 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     4261 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     4321 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     4381 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     4441 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     4501 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     4561 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     4621 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     4681 tgctcatcgc cttgatcata aagcgcaata atttcgggag ccggcaacgg ttgatgataa
     4741 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     4801 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     4861 ggcagacgca tatggccaaa ctcgccggta atgtagcttt tcccggtaat cggtttgccg
     4921 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac caacggatat
     4981 tgcgtaaatt cgtcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     5041 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     5101 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     5161 ccgacagagc ctttacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     5221 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     5281 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     5341 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     5401 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     5461 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     5521 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     5581 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctaccga atagccctgc
     5641 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     5701 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     5761 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5821 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5881 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5941 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     6001 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     6061 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     6121 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     6181 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     6241 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     6301 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacctg gcactttttg
     6361 cacgttatcc agtgcttcct gccagttagt ccacggttga tccaccgcct ctatttcgcc
     6421 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     6481 taagccgacg atcaataccg ccacgccaag ggcaatgcca atggtagaaa tgacggagat
     6541 cagcgacacc atgccgccgc gtcgccgtcc gcggctaaaa cgcaggccaa tcaataacga
     6601 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
     6661 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     6721 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtctgcgtta
     6781 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gccacgggtt attgaccagc
     6841 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6901 gcacgatgct ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
     6961 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcagt aaaatccggc
     7021 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
     7081 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
     7141 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
     7201 gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
     7261 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
     7321 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
     7381 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg
     7441 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
     7501 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
     7561 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
     7621 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
     7681 ggcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa
     7741 cagctcgcct ttacgatcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
     7801 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
     7861 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc
     7921 gacttcactg ttagcggcaa aagtaccaat cacattgaac aggcgctgac ttggaatacg
     7981 ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt
     8041 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
     8101 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
     8161 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
     8221 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
     8281 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
     8341 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
     8401 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
     8461 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
     8521 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
     8581 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
     8641 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
     8701 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
     8761 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
     8821 ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat
     8881 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
     8941 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
     9001 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
     9061 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
     9121 ttaagcaatg cctccaatgc cgcgtatgcc gattccaccg gtgagttcct gcacgggatg
     9181 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
     9241 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
     9301 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
     9361 tacagccttg gtgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
     9421 cgccgccatc tgctgatggc ttctttgctg gctgctctgg cggtcggtgc ctggttgttt
     9481 actatctcgc tgctgttgtc gctggtgtcg attgtggtga tcatgaagtt gttttatcag
     9541 tatgaacaac gttttggcat gcgttccacc agtctgctga atgtgattgg ctccaacact
     9601 attgctatct acactaccca tagaattctg gtagagatat tcagcttaac tctgcttgcg
     9661 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttacccc
     9721 tttgttagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcga
     9781 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ttgccgtcag ttactcccgc
     9841 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
     9901 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
     9961 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    10021 tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg gtagcggaaa ttgccgaacg
    10081 tcacgccgga ccggtggtgc ttattgcgcc agatatgcaa aatgccctgc gtttgcatga
    10141 tgaaattagc cagtttaccg accagatggt gatgaatctg gcggactggg aaaccctccc
    10201 ttacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    10261 gctaccgaca atgcagcgtg gcgtactgat tgttccggtg aatacactta tgcagcgcgt
    10321 ttgcccgcac agttttctcc acggtcatgc gctggtgatg gaaaaaggtc agcgcctgtc
    10381 acgagatgca ttacgaaccc aactggatag cgccggttat cgccatgttg accaggtgat
    10441 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    10501 gttgccttat cgtcttgatt tctttgatga tgaaatcgac agtctgcggg tgtttgacgt
    10561 cgacagtcag cgcacgctgg aggaagtaga agcgatcaac ctgctgcctg cgcacgaatt
    10621 tccgaccgat aaagcggcga ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    10681 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    10741 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    10801 tgccaatacc ttgctggtga atactggcga tctggaaaac agtgccgaac gtttccaggc
    10861 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccac tgttgccacc
    10921 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt
    10981 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    11041 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    11101 cctcgagtct ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggcc gccgtgaagc
    11161 gctgggtgaa ctgctcgcgc gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    11221 agccagcgac cgtgggcgtt atctgatgat tggcgctgca gagcatggtt ttgtcgatac
    11281 ggtacgtaat ctggcgctga tttgcgaaag cgatctgctc ggtgaacgcg ttgcccgtcg
    11341 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc tcgcggaact
    11401 gcatattggt cagccggtgg tccatctgga gcacggcgtc gggcgctatg ccggaatgac
    11461 cacgctggaa gcaggcggca ttaccggcga gtatctgatg ctcacctatg ccaacgacgc
    11521 caaattgtat gttccggtgt cgtcgctgca tctgattagc cgttacgcgg gtggtgcgga
    11581 agaaaatgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    11641 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    11701 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    11761 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    11821 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    11881 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgctggtgcc
    11941 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    12001 ggtacgtatc gaaatgctct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    12061 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    12121 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    12181 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    12241 gccgatccca cgtacgctga atatggcaat gagcggaatg cgtgacctat cgattatcgc
    12301 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    12361 ggtccgcgag gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa
    12421 tgatgtggaa aacattcaga aagccgccga acggctggca gaactggtgc cagaagcgcg
    12481 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    12541 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    12601 ctcgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcaactgca
    12661 ccagttacgc ggtcgcgtcg gacgttcgca tcatcaggca tatgcatggc tgctgacgcc
    12721 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    12781 agatctcggg gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga
    12841 actgcttggc gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    12901 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    12961 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    13021 tcctgacgtg aatacgcgtc tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    13081 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    13141 gcgtaccctg ctggatattg cccgtctacg ccagcaagcg cagaaactgg ggatcaggaa
    13201 gctggaaggt aatgagaaag gcggcgtgat cgaatttgcc gagaagaatc acgttaatcc
    13261 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    13321 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    13381 gtttatgcgt gaactggaag aaaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    13441 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    13501 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    13561 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    13621 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    13681 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    13741 aaaaaatata acgtcggctt tctcgctcta ttacaggcta accccggcgt tgatccttac
    13801 gtaccgcgcg cgggcagcgt gttaacgctc ccgttgcaaa ccctacttcc agacgcgccg
    13861 cgcgaaggca ttgtgatcaa cattgcggag cttcgtctct attactaccc gccgggtaaa
    13921 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    13981 acaatggtga ccaccgtttc tgacaagcgt gcaaacccaa cctggacgcc aacggcaaac
    14041 attcgcgcgc ggtataaggc acaaggaatt gagttgcctg cggtagtgcc ggctggaccg
    14101 gataatccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    14161 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    14221 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    14281 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    14341 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    14401 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    14461 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaacgctg
    14521 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    14581 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    14641 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    14701 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    14761 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    14821 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    14881 gatgaatgac gtcgcaagca attacttaac gatcgataac ta
//
LOCUS       DAZKFZ010000105        14831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000105 DAZKFZ010000000
VERSION     DAZKFZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.105"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(67..348)
                     /locus_tag="ACHOQW_002891"
     CDS             complement(67..348)
                     /locus_tag="ACHOQW_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFP9573941.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(610..1719)
                     /gene="apbC"
                     /locus_tag="ACHOQW_002892"
     CDS             complement(610..1719)
                     /gene="apbC"
                     /locus_tag="ACHOQW_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP9573942.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1851..3884
                     /gene="metG"
                     /locus_tag="ACHOQW_002893"
     CDS             1851..3884
                     /gene="metG"
                     /locus_tag="ACHOQW_002893"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9573943.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4025..4753
                     /locus_tag="ACHOQW_002894"
     CDS             4025..4753
                     /locus_tag="ACHOQW_002894"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFP9573944.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQIKSFAD
                     VAAAEKAKLKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLRKDGEIWKRIADNTRAYDPDNNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIVLFALQL
                     KYKPVKGAVTTIIPPNHEAESLPS"
     gene            6710..7834
                     /locus_tag="ACHOQW_002895"
                     /pseudo
     CDS             6710..7834
                     /locus_tag="ACHOQW_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7847..10832
                     /locus_tag="ACHOQW_002896"
                     /pseudo
     CDS             7847..10832
                     /locus_tag="ACHOQW_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            10842..14471
                     /gene="yehI"
                     /locus_tag="ACHOQW_002897"
     CDS             10842..14471
                     /gene="yehI"
                     /locus_tag="ACHOQW_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP9573945.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            14533..>14831
                     /gene="yehK"
                     /locus_tag="ACHOQW_002898"
     CDS             14533..>14831
                     /gene="yehK"
                     /locus_tag="ACHOQW_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP9573946.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNI"
BASE COUNT         3954 a         3690 c         3614 g         3573 t
ORIGIN      
        1 aaaagccaaa atcattcata ggaatgattt tggcttttta aatatgctta aaacggtgtt
       61 ggcaaattaa tcgtagcgat ttaacacgat tttgcgtttg tcgttattac ccacagctac
      121 cacttctgtc gtaacgcctt ttgcagtaga agaagcacga tagttggttt caactcgttg
      181 taacaactgc ccacaacgtt gctgaagatg attattaaca acatccatac tgcatgtagg
      241 ggaactaatt ctgccattga tggttaatgt tgtagtatca gaagagaacg cgggtgatgc
      301 tagtccataa gcaagaaaaa cagctcctga caaccaatac ttattcattg aacgttatcc
      361 ctgtagtaaa ggttatgccg ggtagattga gtgcataaca cactatagct gtacatccgc
      421 tacacagcca cgagggatga taaagagaca gtggttgcac aatcaatcgc ctttgtacag
      481 tttcggacta agatccactg aatattcgca gtattaaata agtgttcatt aatgcggcaa
      541 aaacaaatac accgcatcaa tataacatct ctgcggtgta tgaagaataa ccagaatggt
      601 taataggcgt tagaccgcgc ggaaggaaat ctcgcctgga atgacttcac cctgccagta
      661 gagctgagct gcaacgcggt cagccagctg gcggtagata gcggtaaatt cgctctccgg
      721 acggctgata accgttggcg tgcctttatc gagatcctca cgtaaggaga tgtgtaacgg
      781 catctgcccc agcagctgtg tatggtattt ttcagccagt ttctctgcac caccggtacc
      841 gaaaatcggt tcatggtgac cacagttact gcaaatatgc acactcatgt tctcgacaat
      901 accgagcacc ggaacctcga ctttctcgaa catcacaatg ccttttttcg catcgatcaa
      961 cgcgatgtcc tgcggggtgg tgaccacgac tgcaccagtt actggaatgt tttgcgccag
     1021 cgtcaattga atgtcaccgg tgcctggcgg catatcaagc accagatagt cgagatccgg
     1081 ccacaaggtt tcctgcaaca tctgcattaa cgccttgctc gccatcggtc cacgccacac
     1141 catcgcattg tcgtcagtga ccagataacc aattgaattg gttgccaggc catgagacat
     1201 aatcggtgcc atatgggtac cgtccggcga agttggacgc tgattttctg cgcccagcat
     1261 ggtgggaatt gatgggccgt agatatcggc gtccagaatg ccgactttcg ccccttccgc
     1321 cgccagtgcc agcgccaggt ttaccgccgt ggaggattta cccacgccgc ctttaccgga
     1381 actgaccgca atgatgttct tcacgccatt aatacccggc tgattcttca cgcgttttaa
     1441 ggtggcgata ttgtgcgaca gtttccagtc gatagctttc gccccggtga tacgcagcag
     1501 ttcggcgcta cattgctctt ttaactcttc aaacgcgcta tgccagacga atggcataac
     1561 cagttccaca tgcaacgtgt cgtccatcca ggcgacatgg tgcaacgctt tcagcgtggt
     1621 gaggttatgc ttcagggttg ggtgctgaaa attggccagt gtcccggcga ccattgcgcg
     1681 caaggcttct ggtgatttgg cctgggattg ttcgttcatc ccgactcctt ttttgtatag
     1741 ataaaccatc agctgatagt ttacctgaag aatatagaga agtacttact taacattttc
     1801 ccatttggta ctatctaacc ccttttcact attaagaagt aatgcctact atgactcaag
     1861 tcgcgaagaa aattctggtg acgtgcgcac tgccgtacgc taacggctca atccacctcg
     1921 gccatatgct ggagcacatc caggctgatg tctgggtccg ttaccagcga atgcgcggcc
     1981 acgaggtcaa cttcatctgc gccgacgatg cccacggtac accgatcatg ctgaaagctc
     2041 agcagcttgg tatcaccccg gagcagatga ttggcgaaat gagtcaggag catcagactg
     2101 atttcgcagg ctttaacatc agctatgaca actatcactc gacgcacagc gaagagaacc
     2161 gccagttgtc tgagcttatc tatactcgct tgaaagaaaa cggttttatt aaaaaccgca
     2221 ccatctctca gctgtacgat ccggaaaaag gcatgttcct gccggaccgt tttgtgaaag
     2281 gcacctgccc gaaatgtaaa tccccggatc aatacggcga taactgcgaa gtctgcggcg
     2341 cgacctacag cccgactgaa ctgatcgagc cgaaatcggt ggtttctggc gctaccccgg
     2401 taatgcgtga ttctgaacac ttcttctttg atctgccctc tttcagcgaa atgttgcagg
     2461 catggacccg cagcggtgcg ttgcaggagc aggtggcaaa taaaatgcag gagtggtttg
     2521 aatccggtct gcaacagtgg gatatctccc gcgacgcgcc gtacttcggt tttgaaattc
     2581 cgaacgcgcc gggtaaatat ttctacgtct ggctggatgc gccgattggc tatatgggtt
     2641 ctttcaagaa tctgtgcgac aagcgcggcg acagcgtaag cttcgatgaa tactggaaga
     2701 aagactccac cgccgagctg taccacttca tcggtaaaga tattgtttac ttccacagtc
     2761 tgttctggcc agcaatgctg gaaggcagca acttccgcaa gccgaccaac ctgtttgttc
     2821 atggctatgt gacggtgaac ggcgcgaaga tgtctaagtc tcgcggtacc tttattaaag
     2881 ccagcacctg gctgaatcat tttgacgccg acagcctgcg ttactactac actgcgaaac
     2941 tctcttcgcg cattgatgat atcgatctca acctggaaga tttcgttcag cgcgtgaatg
     3001 ccgatatcgt taacaaagtg gttaacctgg cctctcgtaa tgcgggcttt atcaacaagc
     3061 gttttgacgg cgtgctggca agcgaactgg ctgacccgca gttgtacaaa accttcactg
     3121 atgccgctga agtgattggt gaagcgtggg aaagccgcga atttggtaaa gcagtgcgcg
     3181 aaatcatggc gctggctgat ctggctaacc gctatgtcga tgaacaggct ccgtgggtgg
     3241 tggcaaaaca ggaaggccgc gatgctgacc tgcaggcgat ttgctcaatg ggcatcaacc
     3301 tgttccgcgt gctgatgact tacctgaagc cggtactgcc gaaactgacc gagcgcgcag
     3361 aagcatttct caatacagaa ctgacctggg atggtatcca gcaaccgctg ctgggccaca
     3421 aagtgaatcc gttcaaggcg ctgtataacc gcatcgatat gaagcaggtt gaagcactgg
     3481 tggaagcgtc taaagaagaa gtgaaagcag ctgccgcgcc agttactggc ccactggcag
     3541 atgatccgat tcaggaaacc atcacctttg acgacttcgc caaagttgac ctgcgcgtgg
     3601 cgctgattga aaacgcagag tttgttgaag gttctgacaa actgctacgc ctgacgctgg
     3661 atctcggcgg tgaaaaacgc aatgtcttct ccggcattcg ttctgcttac ccagatccgc
     3721 aggcactgat tggtcgtcac accattatgg tggctaacct ggcaccacgt aaaatgcgct
     3781 tcggtatctc tgaaggcatg gtgatggctg ccggtcctgg cgggaaagat attttcctgc
     3841 taagcccgga tgccggtgct aaaccgggtc atcaggtgaa ataatccccc ttcaaggcgc
     3901 tgcatcgaca gcgccttttc tttataaatt cctaaagttg ttttcttacg attttgtctc
     3961 tctctaaccc gtataaatac tggtagcatc tgcattcaac tggataaaat tacagggatg
     4021 cagaatgaga cactttatct atcaggacga aaaatcacat aaattctggg cggttgagca
     4081 gcagggaaac gagctgcata tcaactgggg caaagtcggc actaacgggc aaagccagat
     4141 aaaaagtttt gcggatgttg cagcagcgga aaaagcgaaa ctgaagctta tcgcggagaa
     4201 aacgaaaaaa ggctatgtgg aaaatgcttc agcaaacgtg catatccccc ccatcaccaa
     4261 agccactcct gaagttgaaa cttcccctga gagtaaaaac caacgcccgt ggctggctga
     4321 tgatgcagtt attccggtaa ctgacgatat taaccgattt gcttttcctc atcgctctcg
     4381 ccccagagag atcaattatc tgcgcaaaga cggtgagata tggaagcgta ttgcagataa
     4441 caccagggca tatgatcccg acaacaatta ccgctcttat cctgaaaact ggcagcaggc
     4501 ttttgctgag ttacaaaggc gaattctgga taatcaacag acagggagtg cgcaatctga
     4561 tgcagcattg ctctggagtt tttggaactc ttactctgca gatgaactgg tcgatgactt
     4621 agtcattcgc tgtggtctgg aaagcgccgt tgaaatcgtc ctttttgccc ttcaactcaa
     4681 atataaacca gtaaaaggcg cagtaaccac catcattcca cctaatcacg aagcagaatc
     4741 gctacctagt tagcatcaac gtctatgtca tcatctttca ctcgcttcag aagatgagtg
     4801 gcaacgctgt gtagataaag tactcaccgc cattccgacg ctatcccccg cacggcaacc
     4861 ttttgcggcg ttactgctcc ctgaacgccc ggatatcgcc catgctatag atctacgcta
     4921 tgcagaccaa aacgtcccgg cgatgacctg gttaagactg gtgataaccg atgatactgc
     4981 tctgagtaca gtggaaaaat acgacttccc accgttgtat cgtgattttc gtaattttcg
     5041 cgcttatctg gcaatgttgt tggcaaataa tggggtgcgc ggtgtaagcc ggatacttct
     5101 tgagtttacg gaagaccatt ctgacaatcc cacttatctc ttcgaacgca tcagcgagac
     5161 tgaaaatctt gtcaaatggc tatggaaaac gaatcacccg gatgcgattc aaattctgat
     5221 cctcggtgta attggtaaga aaaagcacct ggaatactta agcaaggcca gtcaaaagca
     5281 tcccgctgcg gctattgcgg cttatgctac tttgctggca atacatgaag ataaagagtg
     5341 gcgtaaagcg cttgtcaaac tgattaccgc cacgccagag ttagtatgcg acgtcattcc
     5401 ctgggttaat gctaaagcgg caggcatctt atctgaatgt cgtccgcagt cagtcgctga
     5461 agaatgtgaa tatgccactg tggatatgtt gccggagctg cttgtttcgc cgccctggat
     5521 gaccaaagag aaaaagaaaa atacgcctgt gtttgatctt ccagtgcttc cagttccttc
     5581 tgtttctgat gtcactccag agattaccaa aaaactgacc cgtacttacc ttgtcaccca
     5641 cttccagcaa atagcccaac agcaggcgac aaaacaaaca ttgtttaccg acctgccgcc
     5701 gatcaaaaaa gcaagctggg agaaacactt aataccgctt acaccggagc aacaaatact
     5761 gtggcatctt ggttttgaaa aatggcggga gagcggagaa aaaatatacg aaaaaattcc
     5821 ggcgccacaa agcgccgttg atgccctgct acgcttcgat tttccagcac tcaatgctga
     5881 atttgtgcat tatcataaca atgcatacaa aagctggaat ctcattgccc tctgttacct
     5941 gcctggtcaa caggccattt cttttcttaa ccagatagtc aaagaagata actactctgg
     6001 tgagggaaat attctggcaa tttttggtag cgccgccata cctgcattta tggcctgcct
     6061 gcaacgtgat ccacgacgtc tgtgcttctt cccctttttc ctcggtgtca gtgaactggc
     6121 cttaccgatg gcgcaacaat tacagaaaaa aatgtcttat gaagatgcgc gaaactggct
     6181 aactgattat ccacgccatg ccgccgccgg gctgcttcct gtggccctgg gcaaaaaagg
     6241 aaaagatcgt gattgtgccc gccaggcgct tcgtctgctg gtgaatttaa accagcgaga
     6301 aacgattgag gaaatcgcgc aaggatataa tcaacctgat gttttagctg cgctggcaac
     6361 attgttcgat agcgacccac tggaagaata tcccgctaaa atcgccccac tgcccggctt
     6421 ttatcagttc accttgtggc gcagaccacg gcttaaaagt aacaacctgc ctctgtcaga
     6481 tgacgctatg cgccacctcg gcactatgct gagctttcct cgcgacatta ccgcctacgc
     6541 tgggctggat atcattagag aaatcttcac ccgcgaatca ctggctgaat ttggctggga
     6601 tctgtatccc gcctggacag aagctggcgc acccgcaaaa gaaaactggg catttacttc
     6661 gcttgggatt ttaggcaatg acgacaccgc gcgtaaatta accccgctta tccgcgcctg
     6721 gcctggggaa tcccagcata aacgggcggt gtccgggctg gatgtattag ctgatattgg
     6781 cagcgatgtt gcgctaatgc tgcttaatgg catcgcgcga aaaattaaat tcaaagcatt
     6841 acaggaacat gcccgcgaga aaatcaacat agttgctgaa aaccgtgggc tgactatggc
     6901 tgaactggaa gaccgcctgg ctccagattt agggcttgat agcagtggct cgctgatact
     6961 ggatttcggc ccccgcaaat tcaccgttgg ttttgacgaa accttaaaac ctgtagtgtg
     7021 cgatgcaaac ggcaaagtcc tgaaagattt acctaagcca aaccagagcg atgaaaaaac
     7081 tcaggcaact gacgcggtta atctcttcaa acagttgaaa aaagatgtac gcgccatagc
     7141 cagccagcag attgatcgtc tggaacaggc tatgtgccag cgcagacgct ggacggcaga
     7201 gcagttccgc ctgtttctgg tggagcatcc gctggtacgt cacttaaccc gacggctgtt
     7261 atggggggtt tataacgatg aaaacgccct tatcacctgt tttcgcgtgg cagaagacag
     7321 cacttacagc gatgcgcagg atgagttatt cacgctgcca gcaggaaaca tcggtattcc
     7381 gcatgtgctg gaaatatccc ctgaatccgc cgctgcattc aggcaaattt acgctgatta
     7441 cgaactgctt ccccctttcc aacagctcga gcgaggtagc tatcaccttg ctgataatga
     7501 acgtaatact cacgaactga cacgctggca aggacggctt tgccaggccg gacgcattgt
     7561 cgggctggaa cgcagaggct ggcaacgtct ggaagaaagc ggcagcgttt atgcgatgcg
     7621 caaaacaacc cctcatggcg atctcgaact tgaaacggaa cctttttcat taatttatgg
     7681 tgaaacgggg tatggcgacc agcacccggt tgaaagcgta aaaatcacct cgccagatga
     7741 tcgctacggt aaacaatcct cactcacttt ctccatgctc gacgacatca ccgccagcga
     7801 gctgattaac gacattgaat cactgtttga ttaaaaggat ttaacaatga aaacctatat
     7861 ttatcaggac gaaaaatcac ataaattctg ggcggtggag cagcagggaa acgagctgca
     7921 tattaactgg ggtaaagtcg gtaccaacgg gcaaagtcag gtaaaaagct ttgcagatac
     7981 tgcggcggcg gaaaaagcag cactgaagct gattgcggag aaggtgaaga aggggtatgt
     8041 ggagcaagta gacgctaacg ctcactcaca tgatgcctca gcaaagaaag tcctggtcac
     8101 agaggaaaaa gcctcaataa atcgccaggc agcaactaat ggtttaccct ggctggcaga
     8161 caacgatccc attattcttc ccccgaatat tgcccgccat gccctcagcc accgcctttg
     8221 gccgggagag ccagtaaaaa aaccaaccaa acccgagaaa caaaatttaa tttcccgatt
     8281 ggcaaatcgt acttatcatt attataaaaa tctgactttc gattacagct catgctctga
     8341 tgaatgggct gatgcgatta gccaggcaat cagcttaatt aaggcccaaa agactgagat
     8401 acccgcgcgt actatggcgg tacttacttt tctggaaata tgtttttgtc agggctggaa
     8461 tgatgaaacc tcatccgagt tgctggatga aattgtaagc accttcggta tcgaatacgc
     8521 aactgaaact gtcatcttct ggtggcaaat tgatttcgat tttgactata aagatgaaca
     8581 tttgactttc tttattaatt atgcagatag cagcaaggac agttatagaa ggaataacga
     8641 caaatttagt ttacgtctgc gtaaacattt atcactagca gaagaagaag tatggcaaaa
     8701 ttgcattgca aaattactcg ttgcgttacc agatatatca ctctatcgcc aaccgttaat
     8761 tgccatttta atgccagaaa tacccgaagt ggcccatgag atcgtacatc gtttacacca
     8821 ggtcgcagat gttccacaac ttgaaatgtt gaaactggta gcgacagatc cattcacgct
     8881 cgaaatactg gagaactatc aatacattga tgtatttaac acctatggtg ccagttggtc
     8941 agctacggta ttacgggagc aaggtatagc ggcactggct cgcctgactc cctatgctga
     9001 agaagataat tgcggtgacg tactgaaatg tattaatcat ccgttggcca ttacgaataa
     9061 aaagtgccga aaattttcct tctgcaacga tagtggcatt agccgaagca ttggtaaaaa
     9121 aagatgataa acgcaaacat acattgctgg tttcattact taatgcaaat cctttatttg
     9181 tcgaaaaatt agtcatcgga ttatcgtcgc aagcggcaaa tttattgcaa caatgtcagc
     9241 aattgctgaa tagtcgcaat gaagattttg caaactcaga aattctacca atggtactag
     9301 tctcgccgcc ctgggaaact caaaataaaa ataacgacaa cagtcctgtg ggtaaatacc
     9361 cggcgaaaat ccccccttta ccgttgtttt atcaacctcc attatggact cgcccgaaac
     9421 tcaaaaatag cagcaaggta ctgccagata gcgcactcca gcacctcggt gaaatgctcc
     9481 gcttccctca ggaagaggcc ctgtatccgg gattattgca ggtgaaagat gcctgtaccg
     9541 ccgactcact ggctgaattt gcctgggatc tgtttaccgc ctggctgact gcgggcgcac
     9601 cgtcgaaaga gagttgggcg ttcactgcgt taggcgttct tggtaacgat gacaccgccc
     9661 gcaaactgac gccattaata cgcgcctggc ctggtgaatc ccagcataaa cgcgccaccg
     9721 ttgggttgga tattctcgcc gctatcggta gtgatatcgc cctgatgcag cttaacggta
     9781 ttgcccagaa actgaaattc aaagcattac aggagcgggc aaaagaaaaa attgccggca
     9841 ttgccgaaag ccgcgaactc acggtggcag agcttgaaga tcggttagca ccggatctcg
     9901 gtctggatga taacggttcg ttgctgctgg atttcggctc acgccagttc accgtcagct
     9961 ttgatgaaac cttaaaaccg tttgtgcgtg atgtttctgg cagccgtctg aaagacctgc
    10021 ccaaaccaaa caaaagcgat gatgaatcac aggcgaacga tgctgttaac cgctacaaac
    10081 tgctgaaaaa agatgcgcgt accgtcgccg cccagcaggt agcaaggttg gaatccgcca
    10141 tgtgcctgcg tcgccgctgg tcgccagaaa actttcagct cttcctggtt gaacatccac
    10201 tggtacgcca cttaacccgc cgtctgattt ggggcgttta tagcaccgaa aaccagctac
    10261 tgacgtgctt ccgcgtggcg gaagataaca gctacagcac cgctgacgat gatcttttca
    10321 ccctgccgga aggcgatatc tctgttggca ttcctcacgt tctggaaata tcaccgacgg
    10381 atgctaccgc ctttggtcag ctttttgccg actatgaact gctaccaccg ttccgtcagc
    10441 ttgaccgtaa cagttacgcc ctgacagaag ccgagcgcaa cgccagcgaa ctgacccgct
    10501 gggcaggcag aaaatgcccg agcggtcggg tcatggggct ggcgaataaa ggctggataa
    10561 agggcacccc gcaggatgga ggctggatcg gctggatgat caaacctctg ggccgctggt
    10621 cgttaatcat ggaaatcgat gaaggttttg cggtaggcat gtcgccagcc gaactgagtg
    10681 ccgagcaaat cttaagcaag ctgtggctat gggaaggcaa cgcagaaagc tatggctggg
    10741 ggagtaattc aacacaggaa gcgcagttct ccgtactcga tgccatcacc gccagcgagc
    10801 taattaacga tattgaagcc ctgtttgaat aaggaaagag catggacaag gaattaccgt
    10861 ggctggcgga taacgcccaa ctggaactga aatataaaaa aggcaaaacg ccgctcagtc
    10921 atcgtcgctg gccgggcgaa ccagtgtccg ttatcactgg aagtctcatc cagacattgg
    10981 gagatgaatt actacaacaa gcaggccaga aggaaaatat cacctggaat tatgataaat
    11041 gctcactgga gtggcagtcc gccatccagc aggcaatcaa tttgactggc gagcataaac
    11101 cgtcaatccc agcccttacc atggcggctc tgatctgtat cgcgcaaaat gacagccagc
    11161 agttacttga tgaaatcgtg caacaagagg ggctggaata tgcgacggat gtagtgatat
    11221 cgcgtcagtg cattgcgcgg cgttatgaga gtgactcact ggtcgtcact ttgcagtatc
    11281 aggacgagga ctatggctac ggttatggct ccgcgaccta taacgatttc gatctccgtt
    11341 tgcgtaagca tctgtcgctg gcagaggaaa gttgctggca gcgttgtgcc gataaactca
    11401 ttgccgcact gccaggaata cccaaaattc gccgtccttt tattgcgctg atcctcccgg
    11461 aaaaacctga aatagccaat gaactcgcca gtcttgaaag ctcccggtca agtctccatt
    11521 caaaggagtg gttaaaggtc gttgctactg ataatacggc ggtaaaaaaa ctcgaacgat
    11581 actggggcct ggacgtattt agcgatcgcg aagccagcta catgtcacaa gaaaaccgct
    11641 tcggctacgc ggcctgcgcc tcccttttgc gcgaacaagg actggcagcc gttccgcgcc
    11701 tggcaatgta tgctcataaa gaagattgcg gcagtctgct ggtacaaatt aaccatccgc
    11761 aagtcatccg caccttgctg ttagttgctg ataaaaacaa acccagcctg caacgtgtgg
    11821 ctaaatacag taaaaacttc ccccatgcga cgctcgccgc actggcagaa ctgctggcgt
    11881 taaaagaacc accagcccgc cctggttatc caatcatcga agacaaaaag ctgcctgcac
    11941 agcaaaaagc tcgagaagaa tactggcgta cgctgttaca aacgctgatg gcatcgcagc
    12001 cacaactggc agcagaagtg atgtcgtggt taagtactca agccagggca gtgctgaaga
    12061 gttatttatc ggcactgcca aaaccggtta ttgatggcac cgataacagc aatctgcctg
    12121 aaatcctcgt ttcaccaccg tggcgtagta agaaaaaaat gacagctcca cgtcttgatt
    12181 tggcacggct cgaattaact ccgcaagttt actggcaacc aggcgaacga gagaggcttg
    12241 ccgccactga gtctgcccgt tatttcagca cggaatctct tgcgcaacgc atggaacaaa
    12301 aaagtgggcg agttgtatta caggaactgg gttttgggga tgatgtatgg ctgtttttga
    12361 attatatact ccctggaaaa ctggatgctg cacgcaattc actcattgtt cagtggcatt
    12421 actaccaggg gcgggttgaa gagatcctga atggctggaa ctccccggaa gcacaattag
    12481 cagaacaggc gctccgcagc ggtcacatag aagcgttaat taacatatgg gaaaatgaca
    12541 actactcacg ttatcgtccg gaaaagagtg tctggaacct gtatttattg gcacagttgc
    12601 cgcgtgagat ggctttgact ttctggctgc gtatcaatga gaaaaagcat ctgttcgcgg
    12661 gagaggacta ttttctcagt atcctcggat tggatgcgct accaggtctg ctgttggctt
    12721 tttcacatcg tccaaaagaa acatttccgt taattttaaa tttcggcgca acagaactgg
    12781 ccctgcccgt tgcccgcgtc tggcgacgtt tcgcggcgca gcgtgatctg gctcgccagt
    12841 ggattttaca atggccggaa catacggcta ctgcacttat ccctcttgtc ttcaccaaat
    12901 ctagcgataa aagcgaagct gcattacttg ccctgcgttt actttacgaa catgggcatg
    12961 gcgaattgct acaaactgta gccaatcgtt ggcagcgtac agatgtatgg cctgccctgg
    13021 agcatttact gaaacagggt cccatggaaa tttacccggc acgcattcca aaagcccctg
    13081 atttctggca tccgcaaatg tggtccaggc cgcgccttat cactaataat caacctgtta
    13141 ccgatgacgc tctggaaatt atcggcgaaa tgctgcgctt tacccagggg ggacgttttt
    13201 atagcgggct ggaacaactg aaaacgttct gccagccaca aacgctggca gcttttgcct
    13261 gggatctctt cactgcgtgg caacaagctg gtgcccccgc aaaagacaac tgggcatttc
    13321 tggcgttaag tctctttggt gacgaaagca cggcacggga tctaacaaca cagatcctcg
    13381 cctggccaca aggcaaatct gcccgtgctg tcagcggcct gaacatcctt accctgatga
    13441 ataatgatat ggcgctgata cagctgcatc atatatcgca acgggctaaa tcccgcccct
    13501 tacgtgataa cgcggcggaa tttcttcagg tagtcgcaga aaatcgcggg ctaagccagg
    13561 aagagctagc ggacagatta gtcccaaccc tgggccttga tgatccgcag gcgttgagtt
    13621 ttgattttgg tccccggcag tttaccgttc gcttcgatga aaacctcaac ccggttatct
    13681 ttgatcagca aaacgttcgc cagaaaagcg ttccccggtt gcgcgccgat gacgatcaac
    13741 tgaaagcgcc cgaggcactg gcccgactaa aagggctaaa aaaagatgct actcaggtga
    13801 gcaaaaacct gctcccgcgt cttgaagctg ccctacgtac cacccgacgc tggtcgctgg
    13861 cagattttca ttctctgttt gttaatcatc cctttacccg tctggttacc cagcgattaa
    13921 tatggggggt ttatccggca aatgaaccgc gtcgtttact caacgccttt cgtgtggccg
    13981 cagagggggg gttctgcaat gcgcaagatg agccaattga cctgcctgcg gacgctctga
    14041 ttggcattgc ccacccgtta gaaatgacag cagaaatgcg cagtgaattt gcacagcttt
    14101 ttgccgatta cgaaattatg ccgccttttc gccagttggc gcgccgcacg gtgctgctca
    14161 cacctgatga gtcaaccagt aacagcctga ctcgctggga aggtaaatcc gctaccgttg
    14221 ggcaacttat gggaatgcga tacaaaggct gggagtcagg ttatgaggac gcatttgtct
    14281 atgacctggg tgagtaccgg ctggtcctta agttttcacc cggttttaac cactacaatg
    14341 ttgatagcaa agcgttaatg agcttccgtt ctcttcgagt gtaccgtgac aataaatccg
    14401 tcacttttgc cgaacttgat gtgtttaatt tgagtgaggc gttaagcgca cctgacgtca
    14461 ttttccatta aaaacacagc gggcagtgat gcaactgccc gttatcaaca cgacttacca
    14521 gcggggaaag cgatgatcgt gcaaaaagag ctggttgcta tttacgatta tgaggtccct
    14581 gtacctgaag atccgttttc cttcagactt gagatccata aatgctctga attatttaca
    14641 ggttccgtct atcgactgga gcgattccgg ctacgttcaa catttcatca acgtgatcga
    14701 gaagatgctg acccgctaat aaatgatgcg ttgatttata taagagatga gtgtattgat
    14761 gagcggaaat tacgaggtga atcacctgaa actgtaatag caatttttaa tcgtgaacta
    14821 cagaatatat t
//
LOCUS       DAZKFZ010000106        14458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000106 DAZKFZ010000000
VERSION     DAZKFZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(99..335)
                     /locus_tag="ACHOQW_002899"
     CDS             complement(99..335)
                     /locus_tag="ACHOQW_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP9573947.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(346..1773)
                     /locus_tag="ACHOQW_002900"
     CDS             complement(346..1773)
                     /locus_tag="ACHOQW_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP9573948.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(1773..2366)
                     /locus_tag="ACHOQW_002901"
     CDS             complement(1773..2366)
                     /locus_tag="ACHOQW_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP9573949.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            2513..2920
                     /locus_tag="ACHOQW_002902"
     CDS             2513..2920
                     /locus_tag="ACHOQW_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9573950.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(3040..4032)
                     /gene="rpoS"
                     /locus_tag="ACHOQW_002903"
     CDS             complement(3040..4032)
                     /gene="rpoS"
                     /locus_tag="ACHOQW_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP9573951.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(4095..5189)
                     /gene="nlpD"
                     /locus_tag="ACHOQW_002904"
     CDS             complement(4095..5189)
                     /gene="nlpD"
                     /locus_tag="ACHOQW_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP9573952.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(5374..6000)
                     /gene="pcm"
                     /locus_tag="ACHOQW_002905"
     CDS             complement(5374..6000)
                     /gene="pcm"
                     /locus_tag="ACHOQW_002905"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP9573953.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(5994..6755)
                     /gene="surE"
                     /locus_tag="ACHOQW_002906"
     CDS             complement(5994..6755)
                     /gene="surE"
                     /locus_tag="ACHOQW_002906"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP9573954.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(6736..7785)
                     /gene="truD"
                     /locus_tag="ACHOQW_002907"
     CDS             complement(6736..7785)
                     /gene="truD"
                     /locus_tag="ACHOQW_002907"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP9573955.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(7782..8261)
                     /gene="ispF"
                     /locus_tag="ACHOQW_002908"
     CDS             complement(7782..8261)
                     /gene="ispF"
                     /locus_tag="ACHOQW_002908"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP9573956.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(8261..8971)
                     /gene="ispD"
                     /locus_tag="ACHOQW_002909"
     CDS             complement(8261..8971)
                     /gene="ispD"
                     /locus_tag="ACHOQW_002909"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP9573957.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(8990..9301)
                     /gene="ftsB"
                     /locus_tag="ACHOQW_002910"
     CDS             complement(8990..9301)
                     /gene="ftsB"
                     /locus_tag="ACHOQW_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP9573958.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(9495..9818)
                     /locus_tag="ACHOQW_002911"
     CDS             complement(9495..9818)
                     /locus_tag="ACHOQW_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP9573959.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(9868..10473)
                     /gene="cysC"
                     /locus_tag="ACHOQW_002912"
     CDS             complement(9868..10473)
                     /gene="cysC"
                     /locus_tag="ACHOQW_002912"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP9573960.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(10473..11900)
                     /gene="cysN"
                     /locus_tag="ACHOQW_002913"
     CDS             complement(10473..11900)
                     /gene="cysN"
                     /locus_tag="ACHOQW_002913"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP9573961.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(11902..12810)
                     /gene="cysD"
                     /locus_tag="ACHOQW_002914"
     CDS             complement(11902..12810)
                     /gene="cysD"
                     /locus_tag="ACHOQW_002914"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP9573962.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            13062..14099
                     /gene="iap"
                     /locus_tag="ACHOQW_002915"
     CDS             13062..14099
                     /gene="iap"
                     /locus_tag="ACHOQW_002915"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP9573963.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT         3422 a         3977 c         3649 g         3410 t
ORIGIN      
        1 ttatattgat accggatcgc cgaaaagcgg gcggctgtca ttttataaaa taaagtaatg
       61 aggccgggta aagcaaagcc actacccggc actttttatt agcgcttacc ttccgccagc
      121 agcggcggga tgctcggcac cattggcgcg tcatcaatat ctttctgcgt catgcggaac
      181 gcttcgggat aatgttcgcg gctggtacgg cgcagcggtt cggtatcgcg ccaggtataa
      241 aggcaatgct ggcactgata taccgtccag acatctttca ccggcgattt cgccatcact
      301 tcaatctgtt catcggcaca acgtggacaa atcatcttgt tctccttatt tacgtgcggc
      361 cagcatagcg gtcagttttt cagcccaggc tttggtttcc ggcaaatcca ccaccggctg
      421 gctgtagtga ccacggttgt ccggggcgac aggcgtggtg gcgtcgataa tcagcttgtc
      481 ggtgatcccc gccgggcttg agccagggtc gagttccagc acggacatat tcggcaactg
      541 caccaaatcc cctgccggat ttactttcga ggagagcgcc cacatcacct gcggcaggtt
      601 gaacgggtca acgtcttcat cgaccataat caccatcttt acgtagccca gaccgtgcgg
      661 cgtggtcatc gcacgcaggc ccaccgcgcg ggcaaagccg ccgtagcgtt ttttggtgga
      721 gataatcgcc agcaggccgt gggtgtacat ggcgtttacc gcctgcactt ccgggaactc
      781 ggctttcagt tgttgataca gcggcacaca ggtggctggc cccatcaggt agtcgatttc
      841 ggtccacggc atgccgaggt acagcgattc gaaaatcggc ctggtgcggt aagagacttt
      901 atcgatacgc accacggtca tgttacgccc gccggagtag tgcccggtaa actcaccgaa
      961 cggcccttcg atttcgcgtt tgcggctttc gataacccct tcgaggatca cttctgaacc
     1021 ccacggcaca tcaaaaccag tcaatggcgc ggtggcgatc gggtacgggc tttcgcgcag
     1081 cgcgcctgcc atttcatact cagactgatc gtatttcagc ggcgtggcgc ccataagggt
     1141 gatgatcgga tcgttaccca gggtgatggc aatcggcaga tcttcaccgc gctcttccgc
     1201 tttatgcaga tgcagggcga tatcgtgcat cggcaccggt tgcaggccga gcttacgctt
     1261 gcccttcact tccatgcggt agataccgac gttctgcttg ccgaagttat ccgggtcgag
     1321 cggatcgcgg gaaaccacgc acgctttgtc gagatagaaa ccgccatcac cgtcgtttaa
     1381 acgaaacagc ggcaggatat cgaacagatt aatctcgtca ccatcgacgg tgttctgcgc
     1441 ccaggctgga ttggcgcggc gctccggggc gatcgggaag ttatcccagc ggcggataaa
     1501 ctcatcaatc tgttttttaa ccggggtgtt tggcggcagg cccaaggaaa tcgcgtggtt
     1561 ctgccaggaa ccgatggtat tcatcgccac gcgggcatcg gtaaaaccgc gaatattatc
     1621 aaaccacagc gccggtgcac catcgccgat acgcccggtg gcgttggcag cggcagccag
     1681 atccggttcc gcgttcacct cttcgctgat tttcagtaac tggccgtggt catcaagcgc
     1741 ctgtaaaaag ctgcgtaaat catcaaatgc cattattcat tctcctgaga aaaattccgg
     1801 gcctgcggca atccttgcca gcgcctggcg tagggatgtt caaggccaaa ttgatccagc
     1861 acgcgggcta ccacgtggtg gacaatgtca tctaccgttt cgggatggtt ataaaaggca
     1921 ggcatcggcg gcaccatcgc cacgcccatg cgtgaaagtg cgagcatatt ttcgagatgg
     1981 atggtgctaa gcggcatttc acgcggcacc agcaccagtt tgcggccttc tttaagcacg
     2041 acgtccgccg cgcgccctac caggccatca gcgtaaccag cgcggatacc ggcgagcgtt
     2101 ttcatactgc acggaataac gatcatgccg tctgtacgaa aggaacctga ggagatggtc
     2161 gccgcctgat ccgccgggtt atggctgaag tcagcgaggg cagcaacatc gcgggcgctg
     2221 taaggcgttt ccagttcaat ggtggttttc gcccacttcg acatcaccag atgagtctcg
     2281 acattcggca tctcccgcag cgcttgcagt aatgccacac caagaggcgc accggtagcc
     2341 cctgtcatcc cgacgatcag tttcattgct tactctctta attgttcgta tacgaacatt
     2401 attattaaaa tacgcctctg ttctcattgc ggcaagcact gaaaagtaac aacatcccct
     2461 tcgtcactga aaaaccgcta tgatagcggc agatagtttg cccggagttt acatgacgtt
     2521 acgaaataaa gcgttccatc agttacgaca gctttttcag cagcacacgg ctcgctggca
     2581 gcacgagtta cctgacctga ccaaaccaca gtatgcggtg atgcgcgcca ttgctgataa
     2641 gcctggtatc gaacaggtgg cactgataga agcagcagtc agcaccaaag caacgttggc
     2701 agaaatgttg gcaagaatgg agaatcgtgg cttagtcaga cgtgaacatg atgccgctga
     2761 caagcggcgt cgctttgtct ggctgactgc cgaaggagaa aagatacttg ctgcggcgat
     2821 accgattggt gacagcgtgg atgaggaatt tttggggcgt ttgagtgcag aagagcagga
     2881 gctgtttgtg cagctggtgc gcaagatgat gaacacatag gatgcaaatt gccgggtagg
     2941 acgctgacgt gtcttatcca ggcgacaaaa acacaagctt cccaaacaaa agaaaaaggc
     3001 cagcctcgct tgagactggc ctttctgaca gatgcttact tactcgcgga acagcgcttc
     3061 gatattcagc ccctgcgttt gcaggatttc gcgcaaacgg cgcaggcctt caacctgaat
     3121 ctggcgaaca cgttcacggg tgaggccaat ttcacgacct acatcttcca gtgttgccgc
     3181 ttcgtacccc agcaaaccga atcgacgtgc cagtacttca cgctgtttgg cgttcagctc
     3241 gaacagccat ttgacgatgc tctgcttcat atcgtcatct tgcgtggtat cttccggacc
     3301 gttctctttt tcatcggcca ggatgtccag caacgctttt tcggaatcac cacccagcgg
     3361 ggtgtctacc gaggtaatgc gctcgttaag acgaagcata cggctgacgt catcaactgg
     3421 cttatccagt tgctctgcga tctcttccgc acttggttca tggtccagct tatgggacaa
     3481 ctcacgtgcg gttcgcaggt aaacgttcag ctcctttacg atgtgaatcg gcaaacgaat
     3541 agtacgggtt tggttcataa tcgcccgttc aatcgtctgg cgaatccacc aggttgcgta
     3601 tgttgagaag cggaaaccac gttccgggtc aaacttctct accgcacgga tcagccccag
     3661 gttgccctct tcgataaggt ccagcaacgc cagaccacga ttgccataac ggcgggcaat
     3721 ttttaccacc agacgcaagt tactctcgat catccggcgg cgagaggcga catctccacg
     3781 cagtgcgcga cgcgcaaaat aaacttcttc ttcggccgtt aacagtggtg aataaccaat
     3841 ctcaccaagg taaagctgag tcgcgtccaa cacacgctgt gtggctccct gcgataacag
     3901 ttcctcttcg gccaaatcgt tatcactggg ttcctcttct actaaggcct tttcgtcaaa
     3961 aacctcaact ccgttctcat caaattccgc atcttcattt aaatcatgaa ctttcagcgt
     4021 attctgactc ataaggtggc tcctacccgt gatcccttga cggaacattc aagcaaaagc
     4081 ctggttccgc cgatttatcg ctgcggcaaa taacgcagcg ggtttacgga tttccccttg
     4141 taacgaattt caaaatgcaa gcgtgttgaa ctggttccgg tgctacccat ggttgctatt
     4201 ttttgccccg ccttcacttc ttgttgttcc cggaccagca ttgtgtcgtt atgggcgtag
     4261 gcactcaggt aatcatcatt atgtttgatg ataatcagat taccgtagcc gcgcagcgcg
     4321 ttaccggcat aaacaacacg gccatctgcg gtcgcgataa ttgcctgtcc tttgctgcct
     4381 gcgatatcaa tccccttgtt gcccccctca gaagcgccaa aggtttcgat cactttgccc
     4441 tcagtcggcc agcgccaggt ggagataggc gtactggttg atgtactgct gacagtcggc
     4501 tcggttgtgc ttgctgttgg taccgttaca ggcgctgtga ccgtggtcgc agttggcttg
     4561 ttgttcggca acattttgtt agcactctgt tcacccgaag actcagaata cgtaattgtc
     4621 ggttgcgacg caacagcaac ggtggaattt tgtgcaggct tgatcacaac tccttgctct
     4681 gctgcgtcgg cctgggtaat ggcatttccg ccagtgattg gcgtaccgga agcattaccc
     4741 acctgtaagg tctgaccgac gttcagcgcg tatggtgcct gaatattgtt gcgctgagca
     4801 aggtcacgga aatcgttgcc agtaatccag gcgatataga aaagcgtgtc gccttttttc
     4861 acggtatagg tactgccgct ataactgcct ttcggaatgt tcccatactg acggttatag
     4921 acgatgcgtc cgttttccat ctgtaccggc tgctgagcta ctggctgcac tggctggatt
     4981 tgcggttgtt gagtagcctg aatttgtggc tgctgcaccg gctgaatttg cggttgctgc
     5041 gctgtagacg tcgtccccat tttcggcggc ggcgtaatca acataccaga attggtatgt
     5101 gcaggcgcat tgccattaac ggagctgacc ggtgccggtg gatttgaagt gtcagaacag
     5161 cctgccagcc atagcgaaac cagtgacaaa gccgcaatgc ggcgaacggt gaattttggg
     5221 cttcccgcgc tcatttatcc cccaggaaaa attggttaat aaccagtgac ataattaccg
     5281 tgcaaggcac catactgaac actggaaaag atgttcacga tacgctgacc tgcggcaaaa
     5341 taaccaggaa aaatccaggt atttcctcac gttttaagcc agctcaccct tcactaaagg
     5401 gacaaagcgc acggcctcca cggtatcgat aataaattcg cctccccgac gacgcacccg
     5461 tttcaaatac tggtgctcct cccctacggg taagacgaga atcccgcctt cgtccagctg
     5521 cgtcattagc gcagttggaa tttccggcgg tgccgccgta acaatgatag cgtcaaacgg
     5581 cgcacgtgcc tgccaacctt gccatccatc gccatgacgg gttgaaacat tatgtaaatc
     5641 aagatttttc aggcggcgac gcgcctgcca ctgcaagcct ttaatccgtt caaccgagca
     5701 aacatgctgg acaagatgcg ccaggattgc cgtttgatat cccgaaccgg tgccaatttc
     5761 cagcacccgc gactgcggcg tcagctcgag taattcggtc attcgcgcca ccatatacgg
     5821 ctgcgaaatc gtctgcccct gacctatcgg caaggcgata ttgtcccagg ctttttgttc
     5881 aaacgcttca tcaacgaatt tttcacgcgg cacggcggca agtgcattca gcacctgctc
     5941 atcctgaata ccttgcgcac gtaattgatc cagaagtgct tgtacgcgtc tgcttaccat
     6001 tgcgtgccaa ctcccacgct gtttaaccag tctgaaacca catcttgcgc gctatgcgca
     6061 gttaaatcca catgcagcgg cgtgatggag acatagccct catctaccgc agcaaaatcg
     6121 gtccccggac cggcatcaca tttaccgccc ggcgggccaa tccagtacag cgtattgccg
     6181 cgcggatctt gctgcgggat cacctgatct gccggatgtc gtgtaccgca gcgcgtcacg
     6241 cgaatacctt tgatttgatc caagggtaaa tccggaacat taatattaag aatacgcccg
     6301 gtgcgcagcg gctctttaca cagtgcgcgc aaaattgaac aggttaccgc cgcggcagtg
     6361 tcgtaatgtt tatgcccgtc aagcgagacg gcaagcgccg gaaaacctaa atgacggcct
     6421 tccatcgcgg cggctaccgt accggaataa ataacatcat cccccagatt cggcccggcg
     6481 ttaattccgg acacaacaat gtccgggcgc ggacgcatca gagcattcac gccaagatag
     6541 acgcaatcgg tcggggttcc catttgcaca gcaatatcac cattttcaaa ggtaaacgtg
     6601 cgcagggagg attccagtgt cagagaattt gaagcgccgc tgcggttacg atcgggggcg
     6661 accacctgaa cgtcagcaaa ctcacgcaag gctttcgcca gcgtttgtat accgggtgca
     6721 tgtaccccgt catcattact cagcaatatg cgcataatca cctgttgtgt tgataagttc
     6781 cctgacaacg ctggttgcaa aactacccgc cggaagccag aaacggattt ctacggtgac
     6841 gtcatcccac caattccagc ttaattgttg cggatacagc agcatcgctc tgcgcgcggc
     6901 ttcaactttt tcgcgcacca gtaaagcttg taattcagtt tctgcggcga cagctgcctg
     6961 ttcgaatgcc agcgcttcac gctgagttcc ccattcacca ctgcccggca atgcggcggt
     7021 tatcatcagc tctttatcgt tgacgcgacg ctgtaattcc accagttctt cggtggttgc
     7081 aacaaaccag ctaccacgtc cggctaattg tagcgcatcg ccgtcaacaa cttgattaac
     7141 gtctgctttt ttgaggcgct cagcaacaat ctgattaaac aacgcactgc gggctgccga
     7201 caaccaaaaa ctccgtttat tgcgatcgcg caccggagta ttggtttgcg cccagcgcag
     7261 cgcgccctgc aagttgctac cgccaatccc aaaacgttgg gcaccgaagt agttcggtac
     7321 accttttacg caaatatcga tcagacgttg ttcaacgtca tcgcgattgc tcacttcgcg
     7381 cagaaccagg gtaaaggcgt tacctttcag cgcccctaaa cgcagcttgc gcttgtgccg
     7441 cgcatactcc agcacctggc agccttccaa ttgaaaggcg ctcagatcgg gcatttcctt
     7501 gcccggcacg cgagcgcata accactgttc tgtaacagca tgtttgtctt tttgcccagc
     7561 aaagctgact tcacgggcat gaattttcag gaatttcgcc agtgcatccg ccacaaaacg
     7621 ggtattgcag ccgtttttga ggattctaac cagaatatgc tcaccttcac catcaggctc
     7681 aaagcccaaa tcttccacca ccacaaagtc ttccggattg gctttcagca gcccggtgcc
     7741 ttgcggttta ccgtggaggt aagtgagatt atcaaactca atcattttgt tgccttaatg
     7801 agtagcgcca ccgcttcaca ggcaatccct tccccacgtc cggtaaatcc aagtttttcc
     7861 gtagtagtgg ctttcacgtt aacatcatcc atatggcagc cgagatcttc ggcaataaac
     7921 acgcgcattt gtggaatgtg cggcaacatc ttcggtgcct gagcgatgat agtgacatcg
     7981 acgttgccaa gggtataacc cttcgcctga atacgacgcc aggcttcgcg tagcagctcg
     8041 cggctatcag cacctttaaa tgccggatcg gtatccggga acagtttgcc gatatccccc
     8101 agcgccgccg cgccaagcaa tgcatcggtc aacgcatgga gcgccacgtc gccatcagaa
     8161 tgcgccagca atcctttttc gtaaggaatg cgtacgccac caatgataat tgggccttca
     8221 ccgccaaagg cgtgtacgtc aaaaccgtgt ccaattcgca ttatgtattc tcctgatgga
     8281 tggttcgggt gaggtaaaac tcggccagtg ccaaatcctc cgggcgcgtg actttaatgt
     8341 tatccgcacg gccttcgacc aactgaggat ggaatccgca atattccagc gccgaggctt
     8401 cgtcggtaat agccgcgcct tcatttagag cgcgcgtcag acagtcatgt aacagctcac
     8461 gagggaaaaa ttgcggcgtc agcgcgtgcc ataagccgtt gcgatcaacg gtatgagcaa
     8521 tggcattttt gcccggttcg gcacgtttca tagtatcgcg cactggtgcg gctaggatcc
     8581 ctcccgtgcg gctggtttcg ctcaacgcca acaatcgcgc gaggtcatcc tgatgcagac
     8641 aaggacgagc ggcgtcatgc accaataccc actgcgcgtc gccagcggct ttcaaacctg
     8701 ccagcacgga atcggcacgc tcatcaccgc catctacaac ggtgatttgc ggatgattcg
     8761 ccagaggaag ttgtgcaaaa cggctatcgc caggacttat ggcaatgacg acacgtttca
     8821 cccggggatg cgccagcagc gcatacaccg agtgttcaag aatggtttga ttaccgattg
     8881 agagatattg cttaggacat tccgtttgca ttcgacggcc aaatccggcc gccggaacca
     8941 cggcgcaaac atccaaatga gtggttgcca tgttaattcc cgggctgatt tatcgattgt
     9001 tttgccccgc agactgtgcg cgcttcgacg cgtcaggcac cagacgataa aaagtttcgc
     9061 ccggcctggt catgctgagt tcattacgcg cacgctcttc gagcgcctcc tggccgccat
     9121 tgagatcgtc aatttcggca aaaagttgat cgtttcgcgc tttaagtttc gcgtttgtag
     9181 cttgctgtgc cgccacatca tcattgacgc gggtatagtc atgtataccg ttcttaccga
     9241 accacagcga atactgtagc cagaccagaa tagccagcaa cagcagcgtt agtttaccca
     9301 tcctgccccc tgaaaaacgg catcatcatc ccatgcatcc gaagacgact ctacatcctc
     9361 tgttggggat accgcgacaa cgcgggcaaa tgtaccacat ttgtccattg ttacgtatac
     9421 ccagggcgtg cagaacataa tctcattatt agttacggtt tgaattatga acagaggaga
     9481 caagaaagta caaattagcc cagtagccac ataaacagtg cgccaaacat aatgcctact
     9541 gtcatcaggg tgaaaacaat actgtagcgt agctttccgt ccatcaatga atgcagcgca
     9601 atccccacca ctaccgcaac gggcatcagc gccagaaaga aaggccaggt gtagataaag
     9661 aagaacagcg tgttagagcc ataaatcaac atcggcatcg ccagcgcaaa taaccaggag
     9721 ataaaaccga ccacggcacc aggcagtgac catgtggttt cttcatcctc agtaaggctg
     9781 tcgttatttg ttagtgtaat gttatggcta ttacgcatat ttgatcctgt tactttgacg
     9841 aaccgggcat ggaaacccgg tggtgtctca ggatctgata atatcgttct gtctcaacag
     9901 atctaataat tgctgtacca aatttgttac taattgttca ccattgagat gaatttctgc
     9961 cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag ttgcgcagtt caccggcacg
    10021 cgctttctta tataagcctt ttggatcgcg ggcttcgcaa atcgccagcg gcgtatcgac
    10081 aaacacttcg ataaagcgcc cttctcctac gcgttcgcga accatctggc gttcggcgcg
    10141 gtgtggcgag ataaatgcgg tcagcaccac cagtccggct tcaaccatca aattcgccac
    10201 ttcaccgacg cgacggatat tctctttacg atcggcatcg ctaaaaccga gatcgctgca
    10261 taatccgtgg cgaacattgt cgccatccag cagatacgta ctgacgccga gtttatgtaa
    10321 cgcctcctcc agcgccccgg cgaccgttga tttaccggac ccggagaggc cggtaaacca
    10381 cagcactaca ccacgatgac cgtggtgtag ctcgcgttgt tgcacagtga ccggatggct
    10441 atgccagacg acgttttcgt catgcagcgc cattatttct cccccagcaa atcgcgcgcg
    10501 ccccagtgtg gaaagtggcg gcgaaccagg gcattcaatt ccagttcgaa tgcactaaat
    10561 tcagatggcg cagcagttgc ctggctaact ggctcatgca ccataccggc acctacggtc
    10621 acattgctca ggcgatcgat aaaaatcagc ccaccggtaa ccgggttttg ctgataacga
    10681 tctaacacca gtggctcgtc aaaagtgaga tccaccaggc cgatgccgtt cagcggcagg
    10741 ttttcaactt cgcgttgggt aaggttatta atatcaacct gataacgaat gccatcaaca
    10801 cgagaacgcg tcttcttacc ggcaattttg atgtcgtaac tctggcccgg ggaaagcggc
    10861 tgttccgcca tccataccac atccaccgac gcgctctgca cagctggtaa cgcttcgtct
    10921 gccgccagca gcagatcgcc acggctgatg tcgatctcat ccgtcagcac cagggtgata
    10981 gcttctccgg caaaggcttc ttcgcgatca ccatcaaaag tcacgatccg cgcgacgttt
    11041 gattccacac cagagggcag cacttttaca cgttgcccga cttccacgcg accggatgcc
    11101 agcgttccgg cgtagccacg aaaatcgaga tttgggcggt taacgtactg caccgggaag
    11161 cgcattggct gggcatccac cactcgctga atctccacgg tttccagcac ttcgagcagt
    11221 gtcggaccgc tgtaccacgg catactttca ctttgcgaag ccacgttgtc accttccagt
    11281 gcggagagcg gcacaaagcg gatatccaga ttacccggca gctgcccggc aaaggtcaga
    11341 taatcttcac gaatacgggt gaacgtctct tcactgtaat ccaccagatc cattttgttg
    11401 atcgccacga ccagatgttt gatccccaac agtgtggaga taaaactgtg acgacgggtt
    11461 tgatcgagca cgcctttacg ggcatcgatc agtaagatcg ccagttcaca tgtcgatgcg
    11521 ccagtcgcca tattgcgggt gtactgctcg tgccctggag tgtcggcgat aataaattta
    11581 cgcttctcgg tagagaaata gcggtaggcc acgtcaatgg tgatgccctg ttcacgctca
    11641 gcttgcaggc cgtccaccag cagagccaga tccagctttt cgccctgggt gccgtgacgc
    11701 ttactgtcat tatgcagcga tgagagctga tcttcataga tttggcgggt atcgtgcagc
    11761 agacgaccaa tcagggtact tttgccgtca tcgacgctac cacaggtcag aaaacgcagc
    11821 aggcttttat gttgttgcgc aatcatccag gcttcgacgc cgccttcatt ggcgatttgt
    11881 tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt tttttcagct ccatagaacc
    11941 agcttggtcg cggtcaatca cgcgcccctg acgttcactg gtggtggaaa ccagcatctc
    12001 ttcgatgatc tccggcagcg tttgtgcatt tgactccacc gcaccggtca gcggccagca
    12061 gcccagcgta cggaaacgca ccatccgttt tttaatcact tcgcccggtt gcaggtcgat
    12121 acggttgtca tcaatcatca tcaacatacc gtcgcgttcc agaaccggac gttccgcagc
    12181 gagatacagc ggcacaatgt cgatattttc cagccagatg tattgccaga tatcctgctc
    12241 ggtccagtta gagagcggga aaacgcggat gctttcgcct ttgttaatct gcccgttata
    12301 gttgtgccac agctccgggc gctgattttt cgggtcccag cgatggaagc gatcacggaa
    12361 agagtagata cgctctttag cacgggattt ctcttcgtcg cggcgcgcac caccgaaggc
    12421 ggcatcaaaa ccgtatttat tcagcgcctg cttcaggcct tcggtcttca taatatcggt
    12481 atgtttcgcg ctgccgtgca cgaatggatt aatccccatc gccacgcctt ccgggttttg
    12541 atgcaccagc agctcgcagc cgtaggcttt cgccgtacga tcgcggaact catacatctc
    12601 acggaatttc cagccggtat cgacatgcag caacgggaaa ggcagcgtac ctggataaaa
    12661 cgccttgcgc gccagatgca gcatgacgct ggaatcttta ccgatagagt agagcatcac
    12721 cggatttgag aattctgccg ccacctcgcg aataatgtgg atgctttccg cctccagttg
    12781 ccgcaggtga gtaagtcgta tttgatccat aaccgttcct ttgcaatacc gctattttct
    12841 tgccatcaga tgtttcgact atagggagcg taagagaacg aatgaaatta ccaattagaa
    12901 tgagtagttc cttaacggaa taacgatttg gcaaagctaa tatcaaaaag tgcttaaggc
    12961 accggatttc gggcgtttag gaagatttga aattgtttta gcgcagcggc agtttcatac
    13021 tatggcggta aaaaaatttg catggtattt aaggactcac tatgttttcc gcattgcgcc
    13081 accgtaccgc tgccctggcg ctcggcgtat gctttattct ccccgtacac gcctcgtcac
    13141 ctaaacctgg cgattttgcc aatacacagg cgcgacatat tgccactttc tttccgggac
    13201 gaatgaccgg aacacccgca gaaatgttat ctgccgatta tattcggcaa cagtttcagc
    13261 aaatgggtta ccgcagtgat attcgtacgt ttaatagccg atatatttat accgcccgcg
    13321 ataaccgcaa aaactggcac aacgtgacgg gaagtacggt gattgccgct catgaaggca
    13381 aagcgccgca gcagatcatt attatggcgc atctggatac ctatgccccg cagagcgacg
    13441 cagatgcaga tgccaatctc ggcgggctga cgttacaagg aatggatgat aacgccgcag
    13501 gtttaggtgt catgctggaa ctggcagaac gcctgaaaaa tacgcctacc gagtatggta
    13561 ttcgatttgt ggcgaccagt ggagaagagg aagggaaatt aggcgctgag aatttactca
    13621 agcggatgag tgacaccgaa aagaaaaata cgctgctggt gattaatctc gataacttaa
    13681 ttgttggcga taaattgtat ttcaacagcg gtgtaaaaac ccctgaagca gtaaggaaat
    13741 taacgcgcga cagggcgctg gcaattgcgc gtagtcatgg aattgccgca acgaccaatc
    13801 cgggtttgaa taaaaattat ccgaaaggca ctggatgttg taatgacgca gaaatattcg
    13861 acaaagcggg cattgctgta ctttcggtgg aagcgacaaa ctggaatctt gggaataaag
    13921 atggttatca gcaacgcgca aaaacagccg cattccctgc gggaaatagc tggcatgacg
    13981 taagactgga taatcagcaa catattgata aagcacttcc tggaagaata gaacgtcgct
    14041 gccgtgacgt tatgcggata atgctaccgc tggtgaagga gttggcgaag gcgtcttgat
    14101 gggttggaaa atgggagctg ggtgttctac cgcaggggcg gggaattcta agtgatatcc
    14161 atcatcgcat ccagtgcgcc cggtttatcc ccgctgatgc ggggaacaca gcggcacgct
    14221 ggattgaaca aatccctggg ccggtttatc cccgctggcg cggggaacac aaaccgaaac
    14281 acacgatcaa tccgaatatg agcggtttat ccccgctggc gcggggaaca ctttggtgac
    14341 agtttttgtc actgttttgg tgacggttta tccccgctgg cgcggggaac acactaagca
    14401 tacatatctg tttttaaaca aatttattcc acatcaacaa tctaccaact aaattcaa
//
LOCUS       DAZKFZ010000107        14338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000107 DAZKFZ010000000
VERSION     DAZKFZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2243)
                     /locus_tag="ACHOQW_002916"
     CDS             complement(<1..2243)
                     /locus_tag="ACHOQW_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9573964.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQREDERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQR"
     gene            complement(2215..2544)
                     /locus_tag="ACHOQW_002917"
     CDS             complement(2215..2544)
                     /locus_tag="ACHOQW_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9573965.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(2553..2939)
                     /locus_tag="ACHOQW_002918"
     CDS             complement(2553..2939)
                     /locus_tag="ACHOQW_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9573966.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(3000..3743)
                     /locus_tag="ACHOQW_002919"
     CDS             complement(3000..3743)
                     /locus_tag="ACHOQW_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9573967.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(3754..4155)
                     /locus_tag="ACHOQW_002920"
                     /pseudo
     CDS             complement(3754..4155)
                     /locus_tag="ACHOQW_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
     gene            complement(4152..4730)
                     /locus_tag="ACHOQW_002921"
     CDS             complement(4152..4730)
                     /locus_tag="ACHOQW_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9573968.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(4742..5017)
                     /locus_tag="ACHOQW_002922"
     CDS             complement(4742..5017)
                     /locus_tag="ACHOQW_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9573969.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(5010..5333)
                     /locus_tag="ACHOQW_002923"
     CDS             complement(5010..5333)
                     /locus_tag="ACHOQW_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFP9573970.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(5420..7447)
                     /locus_tag="ACHOQW_002924"
     CDS             complement(5420..7447)
                     /locus_tag="ACHOQW_002924"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9573971.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARISLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(7392..7727)
                     /locus_tag="ACHOQW_002925"
     CDS             complement(7392..7727)
                     /locus_tag="ACHOQW_002925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9573972.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            complement(7849..8973)
                     /locus_tag="ACHOQW_002926"
     CDS             complement(7849..8973)
                     /locus_tag="ACHOQW_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9573973.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVILAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPLSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            complement(8901..9113)
                     /locus_tag="ACHOQW_002927"
     CDS             complement(8901..9113)
                     /locus_tag="ACHOQW_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573974.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(9110..11212)
                     /locus_tag="ACHOQW_002928"
     CDS             complement(9110..11212)
                     /locus_tag="ACHOQW_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9573975.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(11212..11703)
                     /locus_tag="ACHOQW_002929"
     CDS             complement(11212..11703)
                     /locus_tag="ACHOQW_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9573976.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(12378..12530)
                     /locus_tag="ACHOQW_002930"
     CDS             complement(12378..12530)
                     /locus_tag="ACHOQW_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573977.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(12518..12985)
                     /locus_tag="ACHOQW_002931"
     CDS             complement(12518..12985)
                     /locus_tag="ACHOQW_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9573978.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(12982..13479)
                     /gene="rrrD"
                     /locus_tag="ACHOQW_002932"
     CDS             complement(12982..13479)
                     /gene="rrrD"
                     /locus_tag="ACHOQW_002932"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP9573979.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(13479..13694)
                     /locus_tag="ACHOQW_002933"
     CDS             complement(13479..13694)
                     /locus_tag="ACHOQW_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9573980.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            13837..14235
                     /locus_tag="ACHOQW_002934"
     CDS             13837..14235
                     /locus_tag="ACHOQW_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9573981.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            14250..14338
                     /locus_tag="ACHOQW_002935"
                     /pseudo
     CDS             14250..14338
                     /locus_tag="ACHOQW_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3272 a         4237 c         3322 g         3507 t
ORIGIN      
        1 cgctgtcgct gctgatcacc catacccacc gtggcgatat ccaggtcatg ttgctgacgc
       61 tgagcgcgct cttcttcagc cagttgactg gtcagctgaa ttgttttttt cttcagctcg
      121 ttgagtgccg tctgtttctg aagctcctgc tgttttacat ccagcagcgt cagtgcctga
      181 atcagttcat ctttacgggc cagcacactc ttttcatctg ccgtcagttt tttcccgtcc
      241 aggtcgctga tgcgctgctg cagagccaga agctgtttac gcgcttctgt catcctttcc
      301 gtggcaatgc ctgctgactg tctggcagca gcaatctgtc cttccacctg tgcctgttgc
      361 tgactgtact gcagcaataa ccgggtggcc tcatcattac gggtttcgcg tgtttttttc
      421 ttaccggatg ccagggcttt ctcgtaacgt tcatcttcac gttgtatcgc cgcatccctg
      481 acagcctgat cggcgtactg catggcatta atacgcgcta tttcacgctg atgtcgtgct
      541 gcttccgttt cattcatccg gttcagtgca gcattttcag cattacggcg tttctgttgc
      601 tcctgataat tccgctctgc ctgctctttt gcatcctgca aatccttctg gcgttttttc
      661 tcctgaagat cgttaagacg ctgctgatcg tattcaacct gagaagatga tgccgtccag
      721 gggagtcttt tcgcccgcga cactttctcc tgtaaagcgg caatctgttc atccagcgag
      781 tcttcacgac caatattcat ggccgcatcc cagaaacgac tccacaaatc agacagatac
      841 ttcagcgtac tgccaagcgc attgaggtta ttatcaatat ccgcagtacg ccgaccggtt
      901 tcctctgcca gtgcagacat ggctatccgt gcagcatcac tggaccgccc ctgctcccca
      961 aggacgcgta tctgctcaag ctgagtggca gtaagaaaat gcagctcatt gtccagagcc
     1021 ttcgcggcat ttacaggatc atccttcagc cgcttaaact gatttatggt atcgctgacc
     1081 gactggccaa ccgatcgctc catctgtgcg gcagctctcg ccaccatacc gatatcgttt
     1141 ccacgaaatg caccactccc caccacctga gccagcgcac cggctgcagc atgttgcgtg
     1201 ataccattcc cggaaatagc acgactgagc gtccacagct gcccggcagt gactccggca
     1261 taatgccccg tcagcgacag ctggcggtta aattcttccc cctccttctg accgtcatac
     1321 caggctttac ccagaccata gacagccgcg gcaataccgc caataaccgc tcctggcatc
     1381 atgcctttcg gtgacatcag tgtgtctatc cacccggcac ggttagccag cgttattccg
     1441 gatcccctca gcgcacctaa attgccgcgg gccagttcac ctatcagaac gcctatctcc
     1501 tgacgggctg ctgcactttt cagacccagc gaatgcgtgg cttttcctgc ctgctccatt
     1561 ttgcggatat acacttctgc agcactgctt acccccagct gggcagcctt agcacgaagc
     1621 aactcagaag aagaaagatt ctggcgggtt gcctgctctt taagctgacg gataaacgcc
     1681 actttctgtc gggtagcctc ttcctcagcc tgtgtaagaa cacgggtttt cgccgtaacc
     1741 tcagaaatca gcgccagata atcctgctga ccaatcccgc cactgtttct ggcctgtcgg
     1801 atctgttgct gaatacgctg taattcctgc agccccgcac cggcctgttt cacactgtca
     1861 atctgacgat aaaacgcagc agccgcttta tcctgagcct ccgccagagc catggcctgc
     1921 gcctgttcct cgcgcatttt ctggctcagt gcctccatac gctggcgggt ttgctccacc
     1981 tcgcgggcca tgcgttcatg agcctgtgcg ctcttctcca ccgtctgcgc atggacggat
     2041 gcggctgttg cagccgaaga agccgcctgc attgtctgcc gggcggcctg agtctgacgc
     2101 tccataaaac gctgcatacg ggcagaagac cgttctgcat cgctggctgc accattcaga
     2161 aggtttttga tacggggaat ttcattttta aactctgccg catcaatccc caaatcaatg
     2221 accaggttgg ctatctggtc cataacgcac acctccggaa ataccttccc caagatgcat
     2281 cagttcttcg tccgttcgct ccggtatccc gttctcttcc ggtaaaaggc tgaaatcagc
     2341 caccgcagca tcactgctac cggacaccat tctcacgatc aatgccttca gcgaggcaaa
     2401 ctgcgcatcc atccacacat cactgaagct ctgcatccgg aaataatcgc cccactcacc
     2461 aagctcagtg gccgacattt ccgacagcat ccgccgccag tctgcccgcc ggaactcccg
     2521 ggcaagccgc atgacaaact gcatttcccg cgtcaggact tttccggcgt cagcacctca
     2581 tgctccaaat ccccggcatt ctcaatggct cccataccgc tcagcgacag aaccatctcc
     2641 gcccccgctc ccagggcatc atacgaccat gttgtaataa cggatgcgca aagcgtctca
     2701 acatcctgag actgttccgc attccacagt gagcgggaaa ccagccaggc attgatatcc
     2761 atccccatcc gcagaaaagc aatctgtcgt tcagcctccg gcagttctcc ctcttcggca
     2821 tcaaactttg ccgttcgctg ctgaacaaac gccagatatt caattctctg cagcccggac
     2881 agctcactga gcaccacgga ctgcttttca taattaaacg tgccctgttt cagaaacatc
     2941 atgttctcca cctgcaaaaa agccccggat aaccggggca aatgatgagt atcgtcctgt
     3001 taacctgcag cgctgacagt caccgcagcc acagccacaa aattcccgtc agcggtcatg
     3061 cccacaatgc tgacgctgcc ctgcttcacg cctttcaccg tggccacaag tccgttcagg
     3121 gtcacagtgg ctgtctgtgg atctgtcgaa tgcacactga tcgctttgtc actggctccg
     3181 tcaggtttta ctgtaaaggt cagcgtggtg gttgctccca cattgacact ggcggatgcc
     3241 ggtgccaccg tcagcccggt aacgcccaca gtttcagtgc cctcctctgc cagatatgga
     3301 cgccccacac cgctgatttt cactgtgcgg gtcatcacgt cttttgaggc aatggtttta
     3361 cccagtgagc tcagccagcc gcggaaaaca tcaacagtgc cgttgggata tttgatacga
     3421 aacgcgcaga cttcaccgga gtcgaacaac tgaaccagtt ttttctgccc gctgtcaccc
     3481 ggacgccagg ccagcgtcgc cgaagtatca ccaacggatt tctgcccctg ggttgtcgtt
     3541 ttccagtctg catcttcatc atcgagataa gtgtcatctt ctgcatcagc ggtcatttcg
     3601 ccaggttgca gatccttcac catcgcaaga cgcagccagt cagtgtccga caaagggttc
     3661 gcaaacgcat cgccgttgcc ggtatacatc cagaacgtcg tccccgcacc tttcgtcttt
     3721 gccagtggat ttggcgtggt cattaccacc tccttaattc gtgtacgtga tctggtacgt
     3781 gatttccgcc atcgcccagg tggccatctc attatcacgt tgatagttaa aaccgagtgg
     3841 gatcagggtg tcgatgagtc cggaaagtgc cgatacatca ttcagggccg ggaaaatggt
     3901 gctctccatc cacatatcca gctctgaatc cggtgcctgt gcccggatga agacagcaat
     3961 atgcagaaca gcctgccagt catcttcatc cgtcattttt ccggtgtact gagcatcact
     4021 cagccacacc gccacggcag gcagttccta cgcatcaaca aatgccggaa gcccgtcaaa
     4081 aaacgtggcg ctgtctccac actgttcccg aaggcgtgcc agtacgacct ggcggatttg
     4141 tgtatgtcgg ttcatcgggt cagccataac ctcagttgtt gtttcagtgc ataccccagc
     4201 tgtttcggca tttccgcagc aatgatgcgg tcgcgggcat cttcaaatgc ctgtgtcagc
     4261 ggtccggaca gcgggatttt caccacatca atggggtaac gatttttgcc atcaatacgc
     4321 cgcatcacat gccagcgacc attcgccagt tgctgaataa acgcatcccg gaaaagatat
     4381 ttacccacct tcagcacact gccacggtac tgcagttttc caccacgctg ggccagtctg
     4441 acccgggctg tccccagctt aatggcgggc agattgcccc ggttaacgcg gatcctggcc
     4501 gtcatttttc ctgacggact ggctttaaac acccggacac gctgacgaac cagtttcagg
     4561 gggatccctt tcacctggtt atctcccgca acggtattcc cggcaacctg ccgggtggca
     4621 accgagcccg ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata
     4681 cgggtatcaa ggctgttcag attgcggatg gcattctcaa gccccttcat cccacaccgc
     4741 tttactcaat aaagatcatt ggcttaccgt taaagcgttc atgccgtgtg accgtccatt
     4801 gttgtccgtc ataaacaacg cgatccccgc gccgtgggcg gtatcccgaa gaaaacacca
     4861 ccagagagac cgcaggtccg gacagagcat tcagctctgc cagtgtttct cccgggatca
     4921 cagtcatatc gacatcatta atcgaggctg tctttcccat ctttctgacc gtgatagcat
     4981 ccatacgcgc tgccagccgg gaaaagggat cagacattga gttttaccgg cacttcttct
     5041 gcactggttc cggcatctgc ccagacaacc ccgaccagcg gatcagagcc gctgttagtc
     5101 agctgaactt ttcctgactt cagataaacc ttcttacccg ttttcatgtc atccgttttc
     5161 agcttaggca gcataaacac accttcggtc atgccgtcgc ctgtttcacc ctgtggaata
     5221 tcggtcagcg ccaccgcaaa aacatcgccc acctgcacca gatctccgct gctgatggct
     5281 gcactggcaa caatcgccac cgtttttcct tcttctacaa aattctttgc cataactgtc
     5341 tccgcacagc cccgttcagg ggctgatttc aggtacaaaa aaagccctta cgggccatca
     5401 gagttgttgt ctgcgacgtt tacgccgtac atttcaccag accgcggtga tcaactggcg
     5461 cgacaccggc gtcaatacgc actttcgttg tcacgccatc cacactgaag ccctccatct
     5521 gatcaatata tggcgtatcc acaccgttga gataagccac ttcaatcgta tcggagcctt
     5581 ttgacgcagc caggtagaag gtggtctggc tgttatcatc aagacgaggc tctgcaataa
     5641 cggtcgcaaa atctttcacc gggttaataa taccggcgtt aatgtcagcc cccttgacac
     5701 ttgaggagcg aatgacctgg ttagcaacag actccatcgc cgtcggtacc agtacgaacg
     5761 caggacgaat attcagatga cgctccccct ctttctgaac gcgcatcaac tggcgggctt
     5821 tatccagcga tgccacgtcc attgcagcgc tctccagtac gtttgcatgt ttcgctttat
     5881 cgaacagact tacattatct gtggagattt tcgggttaga cgtcagaatg gcataaacca
     5941 gatcggcaat agtggatttc gccgcacggc ccagtttcat cgggacatcg gtcagcatat
     6001 tcagatcatc attgataatg gcctgacggg tgatactgaa cagctcgcca taggtcgcca
     6061 gtgcaatagt ggcctgttta tctccggtgg tgacgtattt atattccgcc ccttcacgca
     6121 cctgacgcag agcactgaag ccccccatac ccacacgatg ggcaatttta aaatcagaca
     6181 actgaccttt ccgcgtccac tgttcatagg tttcaggggc atcttcccag ccctgcagaa
     6241 tggctttgtt cgcaacatcc agcagaatat taccgaagtc agacgtactg tgtgtgaacg
     6301 ccgcaccgac catctgcatc gggttataac tggaaacccc aataccccgt tcagtcagtg
     6361 aaatacgggc atattcacgc agggtcatcc cgttgtagac attatcacgt tcggtttttt
     6421 caaatccggc acgcgccatc agcgcctggc ggatcccatc ccccacaaaa ttaccgtttc
     6481 cggcataaat atgagccggg gtatttttat tagatggcgt ggactcgcgc cccatctcgt
     6541 tcaacagctt ttcgcgggcc tgctccagcg aacattcagg atcggcaaga cactgagcct
     6601 gcagcgtctg ataacgcccg ccaaacatgg caaacagatc attaataccg tttacacgcg
     6661 ctttttgctc tgccagtacc tgcgcacgga tactgttttc atccaccaca ggtgctgctg
     6721 cctgcactgg cgtccgggag gctgcaggtc catcatcctg tacgcgtgga gcactgttgc
     6781 gtggcggagt aatcatgttt cgaatggatt ccggcatctt tttaaattcc tctgtacgtt
     6841 ttgactgaat acatgccatt gccttaacgg ctggcgtcac ctgatcagca aatccatgtg
     6901 ccagacattc ggcaccggac atccaggtct catccgccag catggcagca atttcatcgg
     6961 tggttttccc ggttttctgt gcataagcgg gtaacagaac cgcctcaact ttatcgagca
     7021 ggtcggcata ggtgcgcatg tcctccgcat caccgcccgt aaagccaaat ggtttatgaa
     7081 tcatcatgaa ggagttttcc ggcataatga ccggttttcc caccatcgca atgaccgacg
     7141 ccattgacgc cgccacaccg tcgacataaa cggtaatgga cgcaccatgt gttttcagtg
     7201 cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata ttgagattaa
     7261 tgtgggtgat atcgcccagt gcattcagat cactgataaa ctgcttcgct gtaacacccc
     7321 agaaaccaat ctcgtcataa atataaatat ccgcgtcact ctggtgacca gcctgcatcc
     7381 tgaaccagga attattcttc ggactggtcg tcggtgtgct gcggctcctg tcgtttcgtt
     7441 gcggcactgc tgcctccttt atcactggcc ggatcggtat caaataccag atccagcttg
     7501 cggttttcat caatttcggc cttgcgccga cgtttgacat catccggatt acgaccacct
     7561 gcacgtaccc agtctgattc tgtcgctgct ccaccacgaa tctgaatttt ccaggcctca
     7621 gcctccttaa cagggtcaat ccacggcatc accggtccgg aatacaccgc ggtatacagt
     7681 gaagaacggt caagatcgcg gggtagcctg ataacaccgg atgccacagc ctgtttcagc
     7741 caggcacgat acatcgggcg ggtgacggca ccaataaacc agtcctgcag gatcaggtag
     7801 ccatcagtgg attcaaccag ctcctgacgc tgggcgctgt aagtgccgtt atagttgcgc
     7861 gctgtactgg aaaaaattca gacgactgcc cgccgccacg gcacgcaact gaccattacg
     7921 aaaagtttca aggttaggat tggggcgatc cgacttcacc attccgattt cttcgccggg
     7981 tttcagatcg tcgtaaataa tgcctggctg aatggtaagc tcgcgttcct tatccttgct
     8041 gccattacca tccggttcat agctctgccc atcgcctttc cggatgtaca tccccagagc
     8101 agcggcgatc cttgctgcag tcagctcaga atcctcatac tctttcaggg cactgaggcg
     8161 gatcagcaca ccggacaaca aagacgtccc gcgcatctgg tgcagacggc gaacaaattt
     8221 aagatgcagc attcgctctg catccacttc tttggtttcc atctgccgtc cggataaggg
     8281 acggctttta tacaccagat atttttcggg acgcccccag tcatcaacaa acacgccctg
     8341 attcagcctg ttgctctcat cactggtcat gggaataaag tctggctcga gcgcctccag
     8401 ccagaaatga acaccggcag aaggcgtcag gctgtttatg cgcccggaaa ccatctgggc
     8461 aaacacctca ccatcgcgca gccaggtacg cagcatcaga cgttccagca tcggacgggt
     8521 aaactgcccg gtgacttccg gactgacaga ccattcactc catcgggtgc gtatctccgc
     8581 tgccagatca cgggcaatgg ccccattgcg taataccgga tgtggctcga caataatccc
     8641 gtttttcccc accacccgtt cttccagctt gtcaaataca ccaataacca gatcgtggtt
     8701 gttatcaagg taacgggcct gctcacgtaa cgacacggcc ccgtactggc ttaactggtc
     8761 ggcagttcgg ttttcccgcc gggctttgtg tgtccgcgtc gtttttacgg cctcataagc
     8821 caggatcacc gcacgggaac gcagccttgc cgctttccat cctggtgaaa aaacgccaat
     8881 cacatcatca agaattgcca tcagaacctc gccagccggt acccgggatg cccccgtcgt
     8941 cgtgtaatca gagccgcaag gcggcgctcc cactcctgcc gtccctgccg gatctcagat
     9001 aagttttcca tggtcatctg ctgaccatta aaggtgacgg attttccgtc cagcaccgcc
     9061 atttcagctt ccgtataacg ctgaatcatg gcttcgatat cattctggtt cataaccatc
     9121 ctccggaagt cagccagggg ttaacatcgt cagttactgt tttcttccgt ttttgttttt
     9181 taacaggcgt ggataccggt tccggtgagg gtgacggttc ggtactgtcc gggacactct
     9241 ccagccaggt ttcccggctc gcccaccccg gtgcatccgg ccagcggatc ttttcgtatc
     9301 catgcagaat gaccagagcc tcggcataca ccatcaggtc aaaagcttcg ttggcaccgc
     9361 gacccggctt actccatttc ccgtcactac tccgctcttc atacgtcagt tcgtcgtaaa
     9421 accagctccc cagccagtca gggaaatgca catagccggg acctggagag tcacgccata
     9481 acgcgttatt cacccggtct ttcagtgcat ccgtctgaag aagccagagc ggcacatcac
     9541 ctgcggcctg cgcccgtcgg cccgttcgtc cggtgttatc agggaatgta cgggtgatca
     9601 gttttgcgcg ccggatgctg tcgcccttaa acaggtaaat acgtttacca aggccatcac
     9661 gacggcaacg acgccagaat ttataggcat tatcagtgac cccgtcttca ccgccggagt
     9721 ccaccgccat tgccatcagt cgcatttgtt gagaaggatc ggaggccagc ggccagcttt
     9781 tatgaaaaac atccgtcagc aggacatccc agtcttccgg atagctggcc ggatcaattc
     9841 gctggctctc accgtcgctg tcaccgcgca atgactgcgt gatgttgtaa cgatcaataa
     9901 tccagcgttc gccacggctg ccatagcccg ttacctgaac cacaaaacgg cgatgacgtc
     9961 ccgcctgcac atccactgtc gccacaagga aattaacgcc atccggcaca ctgctggaag
    10021 gaactggctc tgcccgctgc tcaagcagtt cactttttcg ttgctccatg ctggcgcggg
    10081 gaagataagg taatccccag tcggtattga taaccgtctt gagtgtttct tcacttccgg
    10141 ttgtctcgta ttcctgttct gcagtaagca gtttgtaaac gagttgcgag agtgtctggt
    10201 aagcagctgc cggaccctcc atccagaatg acgcaatacg tgagcgtcgg ggatcaccat
    10261 aacgactacc atccgcattg atggattcac catcccgcaa ccagacccca cgtccgttca
    10321 gctcacgttt ttgttcaggc ataatccgtc ctgaacagga aggacactga atataagccg
    10381 cctcacttgc cagcacggga tcggcaatat cacggaaacc agcaaccaca tcgccgcagg
    10441 gctgaaaata ctcaccacag tgtggacagg gccagtacca gcgacggcga tcgccacggt
    10501 tatagagcga cagtatcccc gtggttggtg gagcctcatg cggtgaagtc cgtcgccatt
    10561 tcacatcctt cacatccctg ccgggggaac tctccaccag cgtcatacca ctggacataa
    10621 atgtggtggt acgttttgag gcaagagaga aggcatcccc ctcgccatca atatcttccg
    10681 gaaaacggtc ataatccgtc agcgccacgc atttataatc tgatgaggac atgatattga
    10741 ctgacggcca gccgattttc aggtagttgc cagcaaggaa tgttctgtca taaacgttgt
    10801 tgtcattttt gttcggactc aggcgactga ccacttccgg gctgacgcga aacgttctgg
    10861 cgagtcgttt tttggagtgt tcgcgggctt tttcctccgt catctgaatg atcagcatat
    10921 ccgcaggatc gcaaatcacg ttgtaaatca cccagccgtc aatcaggccg atagtcttgc
    10981 cggttcgtgc cgggccaaca aatatcactg cgtcgtattc acgcgaggcc aggcagttca
    11041 tcggctcaat aacatacggt gccaccagcg gatcccacgg gactgagttc ccggccccca
    11101 tgggcacccg catatactga gcaacggcat cagcaacccg cattcgtctc ggtgcgcgaa
    11161 ggatataacc tgaatcggtt cgtgctgcct ttgcggtttc ctgattcagc attactcctc
    11221 ctgctgtaat tcctcctcat catccgcacc tgcttcggtc acccgcaggg ctatctgatc
    11281 gcgcagatca tcaataatgg actgaacacg gctcacagcg gcaggctgca gaccacagtc
    11341 acgttccaga atatccggta atgtctccag cacctgcacg accgcttttg cccagatggc
    11401 aaactcccgt ctgacatcac tggccggaat gagttgtgcc gtttcctgtt cgaacttaag
    11461 acgctcacgt tcagactgat accaggcttt gcgttcatgt ggatccattt cgccctcagc
    11521 aaccggcggt ggtaacccca taaattcagt cagaatatcg gtcaaccggt atagcttgag
    11581 tttgtcatgt ccaccagcgg gacgaatgtt tttcagtctt gccacgacag tctggcggtg
    11641 cagaccagat aaagccgcca gttgattaat attcagcacc aggtttttca actcatgatc
    11701 catatttcct ccggagagct ttaaacatgc atcgtgcgaa caactttaag aaaacgcgtt
    11761 cgatgtcgaa caaaaaacac tcaattcgac atacaaaaaa caaataacca ttaataatca
    11821 ataagatgca aagatgatgg tggccgataa aaatgcaaaa actagccttt ttccgcgacg
    11881 ctcccgcccc gtggcaggcc accccaccgg gaggagccgt cagcctgaaa gccatgacga
    11941 acgtctgata cagcccttgc atgaatggca tcgggataat ccagaaagga atagcatcga
    12001 cccacaagaa tctgtgtgag tgtcctgttt cttccacccc cgcacaggac tggcgagcat
    12061 gagggacaaa cccgcgaatc attagcgcgg taaaaacccg gtgtgcatcg tttttgatta
    12121 ttcccgcaca ctcgcgcaga aggagttccc cgtcgggcta cggtcatggt taatgcggga
    12181 atacagcgac gatacagcgc atgatgtgtc aggcttgaat acctttatcc gttaaaaggg
    12241 atatcagtta agttatcccg tgtagggtat aagccattat caaagccact ctgtagggag
    12301 tggcttttgt aatggcaata aaaaagcccc gcgaatgcga ggctaaatcc aggtattttt
    12361 aatgactggc tcttatctca acgcagcccc ttaccgcgcg ccagatgctc aatatcaagc
    12421 atcagcaatg agatgtttaa ccatgattca ctccagaagt gagcaccacc ctgtctacag
    12481 agccagatgt gaaggatgat gagtaaaatt atcgctatca tcgaaggcat tgcgtcctga
    12541 tgtattcctg aagcgttctc agtgctgttt ggtcgcggat aattccgtcc cggataccga
    12601 gaacgtttcg tccagcaact ggagagagtt cgacggtggc atcattgccc atgccggagg
    12661 cgctggaggt ttcgtctgag gatggcacag ggcattttcc tttgacgaac acccgaccac
    12721 cattatcaag cttgcgccga agagcatcat ttttagcttt cgcatcagct aactccttcg
    12781 tgtatttagc atcgagtgca tcagcagcac gctggcgctg ctgcatgtca gtaatggtgg
    12841 cggtcgcctg cttcagctca ctgacttttt tatcacgctg ttctttatag gcgatggcgt
    12901 tatcacggta atgattaacc gcccatgact ggcagacgat gatgcagata accagagcgg
    12961 agataatcgc ggttactctg ctcattgttg cccccacaaa cagacttcac gctcaatctc
    13021 gcggcgagtc atcagccctt tccattgctt accgccagcg tatgtccagc gccgtagctg
    13081 atcacatgcg cctttgatat caccctggtt tattttgcga agaagcgtcg atgttctgaa
    13141 attaccagcg cccacgttgt aaacgaacga gtaaagagcg ccgcgcgttg tttccggtat
    13201 atcgactttg atgtacgggt taatttgtct ggcgaccgtg gcaaggtctt tattcaggag
    13261 ggctttgcat tctgcttcgg tatacgtttt accgggaatg atgtcttttc cggtgtgtcc
    13321 gtgacataca gtccatacgc caacgatatc ttcgtatggt atgtagctga caccttccag
    13381 gccatcgtca ccactcggac cagtgatgag cacagacgct atggcagcag ccccaccacc
    13441 aatagcagca gcaacagcct tgcgtaatga tggcgacatt attcacctct cgcagcctta
    13501 cgcttatctt ctttaatctt gaaataaagg tttgtcagat acgtcagcaa gccaaacacc
    13561 aagctaccca atacgcctat tgccacccac tgggatgggg agactttgtc cagcaactgc
    13621 agtagccagt atcccgtccc caccgctgac gtggtgtatg acacacctgt tgttattttt
    13681 tccatctggt acataccccg tctcccgtta tccggaagct gacaacaata aaaagccacc
    13741 agttaattcc tgatggccct gatgcataaa cgtcataata cctgactgtt atgattgaca
    13801 ataatgataa tgtttatata gaaaggttcc cgatgtgtgt tacatatcat ttctccacgg
    13861 ggaatatccc cacgccagcg cagactcttt tacccgttct cttctgcgct ggctcttttt
    13921 tattatgctg ctgcatttac ctctggcacc atgctttcta tctcaacaca atacgtggta
    13981 cttcttgtaa ccaatatcat aacgattaat cgacatagaa tttctcccgt gtacaggaac
    14041 agagttaaaa agccggaacc ggaatcaaat cacaggatga ccatctgcca gtggcaggtc
    14101 ataaaaaaaa ggccgcgcca tgcgcagcca gaactcacaa ggaaaatgat agaaggaaat
    14161 aacattagtg atgtacgcat ggcgcctccc gctaagttct gcaatgatca aacagaactc
    14221 gctacgtgcc cttaaaactc gatcatttag cccctccaag gaggattcac catgcggttg
    14281 attttttaat aaacagtaaa caaaaaagtc aagaattatt cattctgttc tttcatca
//
LOCUS       DAZKFZ010000108        14211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000108 DAZKFZ010000000
VERSION     DAZKFZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            156..1214
                     /gene="citC"
                     /locus_tag="ACHOQW_002936"
     CDS             156..1214
                     /gene="citC"
                     /locus_tag="ACHOQW_002936"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP9573982.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1229..1525
                     /gene="citD"
                     /locus_tag="ACHOQW_002937"
     CDS             1229..1525
                     /gene="citD"
                     /locus_tag="ACHOQW_002937"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP9573983.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1522..2430
                     /gene="citE"
                     /locus_tag="ACHOQW_002938"
     CDS             1522..2430
                     /gene="citE"
                     /locus_tag="ACHOQW_002938"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP9573984.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2441..3973
                     /gene="citF"
                     /locus_tag="ACHOQW_002939"
     CDS             2441..3973
                     /gene="citF"
                     /locus_tag="ACHOQW_002939"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP9573985.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3977..4528
                     /gene="citX"
                     /locus_tag="ACHOQW_002940"
     CDS             3977..4528
                     /gene="citX"
                     /locus_tag="ACHOQW_002940"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP9573986.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4503..5381
                     /gene="citG"
                     /locus_tag="ACHOQW_002941"
     CDS             4503..5381
                     /gene="citG"
                     /locus_tag="ACHOQW_002941"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP9573987.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5432..6895
                     /gene="citT"
                     /locus_tag="ACHOQW_002942"
     CDS             5432..6895
                     /gene="citT"
                     /locus_tag="ACHOQW_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP9573988.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7009..7815
                     /gene="rna"
                     /locus_tag="ACHOQW_002943"
     CDS             7009..7815
                     /gene="rna"
                     /locus_tag="ACHOQW_002943"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP9573989.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8045..8455
                     /gene="rnk"
                     /locus_tag="ACHOQW_002944"
     CDS             8045..8455
                     /gene="rnk"
                     /locus_tag="ACHOQW_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP9573990.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8593..8682
                     /gene="yldA"
                     /locus_tag="ACHOQW_002945"
     CDS             8593..8682
                     /gene="yldA"
                     /locus_tag="ACHOQW_002945"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP9573991.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8686..9924)
                     /gene="ybdR"
                     /locus_tag="ACHOQW_002946"
     CDS             complement(8686..9924)
                     /gene="ybdR"
                     /locus_tag="ACHOQW_002946"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP9573992.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10145..10573
                     /gene="uspG"
                     /locus_tag="ACHOQW_002947"
     CDS             10145..10573
                     /gene="uspG"
                     /locus_tag="ACHOQW_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP9573993.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10694..12259)
                     /gene="ahpF"
                     /locus_tag="ACHOQW_002948"
     CDS             complement(10694..12259)
                     /gene="ahpF"
                     /locus_tag="ACHOQW_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9573994.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12388..12951)
                     /gene="ahpC"
                     /locus_tag="ACHOQW_002949"
     CDS             complement(12388..12951)
                     /gene="ahpC"
                     /locus_tag="ACHOQW_002949"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9573995.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13323..14069
                     /gene="dsbG"
                     /locus_tag="ACHOQW_002950"
     CDS             13323..14069
                     /gene="dsbG"
                     /locus_tag="ACHOQW_002950"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP9573996.1"
                     /translation="MLKKILLLALLPAIAFAEKLPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3390 a         3746 c         3562 g         3513 t
ORIGIN      
        1 gaaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt
       61 tctcccaagt gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat
      121 cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc
      181 gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg
      241 atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta
      301 tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc
      361 gcggtgaagg actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc
      421 acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg
      481 gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac agcgccacgc
      541 gactgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct
      601 gcattgcgat gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg
      661 cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct
      721 atgaagaccg gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc
      781 atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac
      841 agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg
      901 ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg
      961 cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca
     1021 tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg
     1081 tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca
     1141 cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa
     1201 agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac
     1261 ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct
     1321 gcaaatcaat agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga
     1381 cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgata aaggcgcact
     1441 ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc
     1501 tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc
     1561 cgccgcagca tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc
     1621 tacccggctg atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac
     1681 accgcccgcc gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc
     1741 attgtgcgtg taaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt
     1801 cgcggtggtg cggacgttgt acgtctgccg aaaaccgata ccgctcagga tgttctggat
     1861 atcgaaaaag agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc
     1921 ctgctggcgg cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct
     1981 tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca
     2041 gaacgctccc cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg
     2101 cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga
     2161 ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac
     2221 ccgcgtcaga ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac
     2281 gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc
     2341 ctgaacggca agatggtgga cggtccggtt atcgatcgtg cccgtctggt gctctcccgt
     2401 gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca
     2461 atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg atcttgccgc
     2521 tttccagaac tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg
     2581 cgccaacctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga cggtttcctt
     2641 ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa
     2701 gatgggcttt aaaaacctga tcctggcgtc cagctccctg agtgattgcc atgcgccgct
     2761 ggtagaacac attcgccagg gcgtggttac ccgcatttat acctccggcc tgcgtggtcc
     2821 actggcggaa gagatctccc gtggtctgct ggcggaaccg gtgcaaatcc actctcacgg
     2881 cggacgtgtg catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt
     2941 cccgtcctgt gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc
     3001 cctcggctat gcaatggttg atgccgacaa cgcaaaacag gttgtgatgc ttaccgaaga
     3061 actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg atttaatcgt
     3121 caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggtgctaccc gtatgaccac
     3181 taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtca actcaggcta
     3241 cttcaaagaa ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg cggtaaccct
     3301 tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggtggtat
     3361 taccgcgacg atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtaca
     3421 gagctttgac agccacgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag
     3481 cgccaaccag tacgctaact ggggttcgaa aggtgcatcg gttgatcgtc tcgacgtggt
     3541 ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga ccggctctga
     3601 cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc
     3661 catcatcgtc gcgccgctgg tacgcggtcg tattccgact ctggtggata acgtgctgac
     3721 ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa
     3781 cccggcacgt ccggaactgg cagaacgtct gcaggaagcg ggcattaaag tggtttccat
     3841 tgagtggctg cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac
     3901 cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca
     3961 ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat
     4021 tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct ggctcaagcg
     4081 ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagtga
     4141 ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag ccgcaaaaca
     4201 gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg agggtatgtt
     4261 gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca
     4321 tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg
     4381 ccgcgactat tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg
     4441 cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa
     4501 cgatgtcgat gcctgcaacg ttaactaaaa ccacaaagct tgcgacgtca ttaatcgatg
     4561 agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag
     4621 gtctcgtgga tcgcattaac tgcggtgcgc ataaagatat ggcgctggaa gatttccacc
     4681 gcagcgcgct ggcgattcag ggctggctac cccgtttcat tgaatttggt gcctgtagtg
     4741 cggaaatggc accagaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag
     4801 gtgatatgtt ccgcgccacc gcgggcgtaa acacgcataa gggcagcatt ttttctttag
     4861 ggctactgtg tgcggcaatt ggccgtttgc ttcagctcaa ccagttgcta acgccaacaa
     4921 ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca
     4981 ataattcaca actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac
     5041 gcggtgaagc cgaagcgggt tatccactgg tgatcaacca cgccttgccg cattacctca
     5101 ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga
     5161 tggcgatcaa cggcgatacc aacgttgctt cgcgcggtgg cgaggggggc ctgcgctggc
     5221 tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc gcagatctcg
     5281 attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg
     5341 ccgacctgct gatccttacc tggtttttag cacagattta attatttaag aacttgataa
     5401 atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa
     5461 actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat
     5521 gccgccgcag gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct
     5581 cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag
     5641 taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca
     5701 ggcgctgaaa tggggcctgg ctggtttttc cagcactacg gtatggctgg tatttggcgc
     5761 atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct
     5821 ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat
     5881 tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt
     5941 cattaaaaac ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat
     6001 tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt
     6061 caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca
     6121 gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat
     6181 tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc
     6241 aacctgggcg ggtgatgaac taaaaaccat gggtgcgctg acacgcagag agtggacgct
     6301 gattggcctt gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc
     6361 tactgcggtt ggtctgctgg cagtttcgct aatgctggct ctgcacgttg tgccgtggaa
     6421 agacattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt
     6481 gatggctaac ggcctgactc gttctggttt tattgactgg ttcgccggta ccatgagtac
     6541 gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt
     6601 tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct
     6661 ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct
     6721 gtctatcggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta
     6781 cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat
     6841 ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt
     6901 tcgccaaacg ctgaataaat catcacgccc gccaggttag cccacctggc gggctttttt
     6961 atgatttaat agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc
     7021 tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc caacgccgta
     7081 gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa
     7141 accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg
     7201 caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg gccaggattg
     7261 cctaaatcgg ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct
     7321 actcgcccaa tcccgaatct accagaagcg cgcgccagcc ggatgtgttc atcgccggaa
     7381 accggattat cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga
     7441 cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg
     7501 gacgcatact tcggtacgat ggtacgcctg aatcaagaga taaaagagag cgaagccgga
     7561 aaattccttg cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt
     7621 gccaaaagct ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct
     7681 gcgtatttga ctgaaattca ggtctcgatc aaagctgacg ccatcaacgc tccgctttct
     7741 gcaaactcat tcttgccaca accacaccca ggtaactgtg gcaaaacctt tgtgattgat
     7801 aaagcgggtt attaatcctc tgatttacaa aacacgctaa ttcgtctttc ataaaccatg
     7861 acgattaaga attatacata atgcgctgca ttgaggaatt ccctgaatgt gacgcaaatc
     7921 acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg ccgcctgacg
     7981 gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac gacatggagt
     8041 aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca tcgatattct
     8101 gctggagcaa cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg cagagttgga
     8161 tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa tgaacagccg
     8221 ggttaaattc cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg tgtatccggc
     8281 aaaaatgacc gatagcaata ctcagctttc cgttatggct ccggtaggtg ccgcactgct
     8341 gggactgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg caacccacct
     8401 tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc tttaatccca
     8461 tctgtacaag cgataactcg ctttgcccag aggatgcact cgcatcctct ttatgcaaaa
     8521 tcagcaattt ttcctggttc tcactgttca cttttgctgc aaaattattc cgtttcgttt
     8581 gtgggaataa ccatggcaga agcattttac atcctgatag gatttctgat tatggcggcg
     8641 attatcgtca tggctgtcct ttacctcgaa aaccactcct gacgctcata ttgttccccc
     8701 cggcatcgca ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc tttgtgcacc
     8761 gggtaccaga atcaccttac ggcactcctc ttcacgcttt tcaaagatct catatccccg
     8821 ggcggcctct tcaaacggca tatagtgggt aacaatttct tctggtttca gtaatccttt
     8881 ctcaattaac ggtaataatt ctcccagcca tgcgtgaacg tgggtctgtc ccattttaaa
     8941 cgtcaaccct ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc cagcgtagac
     9001 gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactgac gcaacgcttt
     9061 accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc tgcctttcgc
     9121 ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg tttgttcaat
     9181 aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc cgtagcggtc
     9241 agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt ccgcaccgag
     9301 caaccgtgca caggcgattg tcaacaatcc cacaggaccg gcaccataga cagcaacgct
     9361 tgaaccttgt tggatctgcg cattttttgc tgcctgccat gccgttggca gaatatcaga
     9421 aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc ccacattccc
     9481 tttagggaca cggacatact ccgcctgtcc accaggaacg ccaccataca ggtgactata
     9541 accaaacaat gccgcgggag ctggtatctg ttttttattg agcgcagcgc ctttacccgc
     9601 attggtattt tcgcaggcgg catattgctg cagtcgacag aaaaaacagt cgccacaagc
     9661 aatgacgaac ggaattacca cccggtcgcc tttttgcaaa ttttttacat cctttccggt
     9721 ttcaactact tcccccataa attcatgacc aaaaatatcg ccatgtttaa cctggggtat
     9781 tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttgccgtta tacgcagaat
     9841 aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa cgtgatgtgg
     9901 gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa gagtcagtct
     9961 ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg agaattcctg
    10021 gtgttcagtg agaatatatt catcacagaa acagttcgta aggaaaaaga accagacaaa
    10081 tatcagggat aaatattata atcatggcga atgatttgtt catgattaac agggagaaag
    10141 ggttatgtat aagacaatca ttatgccagt tgatgtattt gaaatggaat tgagcgacaa
    10201 agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt
    10261 actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc gtcgttttga
    10321 agagcatctg caacatgaag cagaagaacg tttgcaaacg atggtcagcc acttcaccat
    10381 cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg aagtcaatga
    10441 gttagcagaa gaactggggg ctgatgttgt agttattggt tctcgcaacc catcgatttc
    10501 gacccatctg ttaggttcta acgcctcgag cgtgatccgc cacgccaatc tgcctgtact
    10561 ggtcgttcgc taacagataa aagtatttat gccgccctgc ctggaacatc ccggcagggc
    10621 tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttgcaggtga
    10681 atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg
    10741 ctttggcacc ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc gtacagtcac
    10801 ccgctgcgaa cacgcctttc acgttggttt cgcatttcgc atcaatgata atctcgccca
    10861 tgcggttacg ttcgactgcg ccttcgagcc agttggtgtt cggcagcaga ccaatctgca
    10921 cgaaaatacc ggccagttcg atgttgtgaa tatcgccgct gacacgatct cgatattcca
    10981 gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt
    11041 cgacgttttt caggctgcgc agtttgtcct gcagaacctg gtcggctttc atttctggcg
    11101 caaattccag cagcgttacg tgctcaacga tacccgccag gtcaattgcc gcttccacgc
    11161 cggagttacc gccgccgata accgctacgc gtttaccttt aaacagcggg ccgtcgcagt
    11221 gcgggcagta ggtcacgcct ttggtgcgat actgatcttc gcccggaacg ttcatgttgc
    11281 gccattttgc accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg
    11341 tttcaatctg atgcagacca ccttcaactg ctgctgggat cagtttgctg gcgctctggc
    11401 tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct gccagcttct
    11461 gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac
    11521 caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg
    11581 ccgctgcacc cgccgggccg gaaccgacga ttaatacgtc ataagcatca cgcttgttca
    11641 gctcttctgc cgcacgtttt tccgcgcccg tatcaatttt ggcaacgatt tcagtcaaag
    11701 tcatgcggcc ctgaccaaac tctttcccgt tcacgaacac tgccggaacg cccatcacgt
    11761 tacgatcagt gatctcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg
    11821 ggttcaatac gctcatcagg ttcagcgcct gtaccacgtc cgggcagtta tggcaagaga
    11881 gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag
    11941 actgcgcttc tttcgacgga tgaccaccgg tccacagtaa cgccagtacc agcgaggtga
    12001 actcgtggcc cagcggggag cctgcaaaac gtggcccctg gttagaacct gggttggtga
    12061 tcaggaaaga cggcttacgc accggcaagc tgttatcttc tttaaaggtg actttgtctg
    12121 acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca
    12181 gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt
    12241 tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct
    12301 ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc
    12361 acccgattaa ttacgcgatt gcggaactta gattttacca accaggtcca gagacggagc
    12421 cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt gagaagctac
    12481 gtactgtgct gctttgattt tacgcagcag gtcagacgcg tcacggccaa tgccttcagc
    12541 ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg cacggtcagc
    12601 cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc
    12661 gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt
    12721 gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca gttcttcgta
    12781 gtggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag ccgggtagaa
    12841 gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga attcgccgtt
    12901 tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca tctatacttc
    12961 ctccgtgttt tcgatgagat gtaaggtaac caatttttac cgattcggct aatgcgtttc
    13021 cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg tgaactttgc
    13081 accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg taatctgaac
    13141 taccctttgg tatcttcagc gccagtctgc aggctgcgtt gttgctctta caaatgcaac
    13201 aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct aagctcttac
    13261 ctatgacaat gataggttat gccttttact cgacttttgc actgactgaa aaggacaaat
    13321 taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc gcagagaaac
    13381 ttcctgctcc agtaaaagcg attgaaaaac agggcattac aatcatcaaa acgttcgatg
    13441 cccccggagg aatgaaaggt tatctcggaa agtatcagga tatgggcgtc accatctacc
    13501 tgactccaga tggtaagcac gctatctctg gttacatgta caacgagaaa ggtgaaaacc
    13561 tgagtaacac acttatcgaa aaagaaattt acgcaccagc cggacgcgaa atgtggcaac
    13621 ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc attgtctacg
    13681 tcttcgccga tccgttctgc ccatattgta aacagttctg gcagcaggcg cgcccgtggg
    13741 tagattctgg caaagtacag ttgagaacat tgttggttgg ggttatcaaa ccagaaagtc
    13801 cggcgacagc cgcggcaatt cttgcctcca aagatcccgc aaaaacctgg caagaatatg
    13861 aagcctctgg tggcaagctt aagctaaacg tgcctgcaaa cgtaagtaca gagcaaatga
    13921 aagtgttaag cgacaacgag aaactcatgg acgatctggg ggcaaatgtc actccggcta
    13981 tctattacat gagtaaggaa aatacgctgc aacaggccgt ggggttgccc gatcagaaaa
    14041 cgcttaatat cattatggga aataaataaa aggtaattca tggatggttg aactatatcc
    14101 ctgcttttca accatcctaa aaaagcatga attgctcgct ctgccctaag tggctgtgct
    14161 attttagaaa aacttaatcc tgagttaagc aaaaaaaaca taatcaataa a
//
LOCUS       DAZKFZ010000109        14134 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000109 DAZKFZ010000000
VERSION     DAZKFZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(260..775)
                     /gene="pgpA"
                     /locus_tag="ACHOQW_002951"
     CDS             complement(260..775)
                     /gene="pgpA"
                     /locus_tag="ACHOQW_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9573997.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(753..1730)
                     /gene="thiL"
                     /locus_tag="ACHOQW_002952"
     CDS             complement(753..1730)
                     /gene="thiL"
                     /locus_tag="ACHOQW_002952"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP9573998.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1808..2227)
                     /gene="nusB"
                     /locus_tag="ACHOQW_002953"
     CDS             complement(1808..2227)
                     /gene="nusB"
                     /locus_tag="ACHOQW_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9573999.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2247..2717)
                     /gene="ribE"
                     /locus_tag="ACHOQW_002954"
     CDS             complement(2247..2717)
                     /gene="ribE"
                     /locus_tag="ACHOQW_002954"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP9574000.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2806..3909)
                     /gene="ribD"
                     /locus_tag="ACHOQW_002955"
     CDS             complement(2806..3909)
                     /gene="ribD"
                     /locus_tag="ACHOQW_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP9574001.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3913..4362)
                     /gene="nrdR"
                     /locus_tag="ACHOQW_002956"
     CDS             complement(3913..4362)
                     /gene="nrdR"
                     /locus_tag="ACHOQW_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9574002.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4513..5052
                     /locus_tag="ACHOQW_002957"
     CDS             4513..5052
                     /locus_tag="ACHOQW_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9574003.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5351..6235
                     /gene="tsx"
                     /locus_tag="ACHOQW_002958"
     CDS             5351..6235
                     /gene="tsx"
                     /locus_tag="ACHOQW_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9574004.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6412..6759)
                     /gene="yajD"
                     /locus_tag="ACHOQW_002959"
     CDS             complement(6412..6759)
                     /gene="yajD"
                     /locus_tag="ACHOQW_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP9574005.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6847..7128)
                     /locus_tag="ACHOQW_002960"
     CDS             complement(6847..7128)
                     /locus_tag="ACHOQW_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9574006.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7121..7363)
                     /locus_tag="ACHOQW_002961"
     CDS             complement(7121..7363)
                     /locus_tag="ACHOQW_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9574007.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7515..8486)
                     /gene="secF"
                     /locus_tag="ACHOQW_002962"
     CDS             complement(7515..8486)
                     /gene="secF"
                     /locus_tag="ACHOQW_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP9574008.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8497..10344)
                     /gene="secD"
                     /locus_tag="ACHOQW_002963"
     CDS             complement(8497..10344)
                     /gene="secD"
                     /locus_tag="ACHOQW_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP9574009.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10372..10704)
                     /gene="yajC"
                     /locus_tag="ACHOQW_002964"
     CDS             complement(10372..10704)
                     /gene="yajC"
                     /locus_tag="ACHOQW_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9574010.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10727..11854)
                     /gene="tgt"
                     /locus_tag="ACHOQW_002965"
     CDS             complement(10727..11854)
                     /gene="tgt"
                     /locus_tag="ACHOQW_002965"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9574011.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11909..12979)
                     /gene="queA"
                     /locus_tag="ACHOQW_002966"
     CDS             complement(11909..12979)
                     /gene="queA"
                     /locus_tag="ACHOQW_002966"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9574012.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13072..13653
                     /gene="acpH"
                     /locus_tag="ACHOQW_002967"
     CDS             13072..13653
                     /gene="acpH"
                     /locus_tag="ACHOQW_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP9574013.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13674..>14134)
                     /locus_tag="ACHOQW_002968"
     CDS             complement(13674..>14134)
                     /locus_tag="ACHOQW_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574014.1"
                     /translation="INEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLHNVARNGS
                     PRYAGLLRQIASKSAILQDFDQQLFWRTFTGGVLHRHISVKSEPEKTDVWRFSSQFDF
                     PHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANAKK"
BASE COUNT         3521 a         3795 c         3399 g         3419 t
ORIGIN      
        1 cgaaaccatt gagaataatt ttgatgatat ttttcgtcaa cagtcagaga atatgagcaa
       61 ttattatgag ttcgtcgact gacgatattt cagtgtcatt gaattatgag tgacaaagat
      121 acaccttgaa atatcgccgc atagtatatt cggcgatgtc gggttttact ttaacagagt
      181 gccccaaaat tggggcactg ttcatttata aatcaaaccg ggtggtctgg aatcgcctac
      241 acctcccggt caatggcatt taaagaatac ccagcggcca gtgatgaccg ataaagtaca
      301 ggatgcctgc ggaaatcacc ccggcgacaa tatcatcgat catgatcccc atgccgccat
      361 gtacattgcg atcaaaccag cggatcggcc acggcttcca catatccaga atacggaaaa
      421 tcacaaaccc ggcggcgacc cactgccagt cattggtcgg cagcgccatc agggtgatcc
      481 acatgccaat aaactcatcc catacgatgc taccgtgatc gtgcaccccc atgtctttcg
      541 ccgtttgatg acaaagatag acgccgatac agatccccaa catcaccacc agcgagtaga
      601 gctgccaggg caaaaaggtc atcagatacc agaacggaat cgccgccagc gatcccatcg
      661 tgccaggaac gattgggctt aatccacttc cgaatccgac agcaagcaga tgccacggat
      721 tactcatctt caggcgactt ttcgcgacat ctttatggcg tggcaaaatg gtcatatcct
      781 ttccagtcta acgtgacagg ttcgccgtca cgaataaaac aaagcccttc gatatcggcg
      841 gtcatttgcc cgatacaggt aaacggtacg cccagatgac cgagagccac atccagcgcg
      901 ccacggttca gttccggcac cgtgaaacac aactcgtaat cttcaccgcc agagagcgcc
      961 cagcgcagcg cctgttccgg tacaacatgg cgagaaagcg catcagaaaa cggcagcaat
     1021 gccaggtcaa tacgtgcgcc gcagtcgctg gctttcacga tatgcccgag atcggaaatc
     1081 agaccgtcag agagatcgat agctgaattt gccagatcgc gcagtgcctg cccctgtaaa
     1141 atacgcggcg atggacggag atgacgtttg atcaagtagt ccgcatcttt agcatcggca
     1201 acctgcaaac ggttttgcaa aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc
     1261 acatagatcc agtcacccgg tttcgcccca gagcgcgtta aggctcgtcc catcggaaca
     1321 aagccgtgga tacccaacgt cattgataat ggcccacgcg tggtatcgcc gccaatgagt
     1381 tgcatatcgt aataattgag aagatcaaac aaactgtcgc tgaaggactc aagccacgct
     1441 tcgtctacgt ccggtaaggt taatgccagc gtcagccagg ccggatcggc ccccatcgct
     1501 gccagatcgc ttaggttcac cgccagtgct ttataagcca gatcagcagg atcgatatca
     1561 gggaggaaat ggttgcccgc caccagcgta tcagtgctga tcgccagggt ctgtttctcg
     1621 gggatattga gaagagcgca atcgtcgccg atgcccagtt cgacatcaag acgagaactt
     1681 cttacacggt caaaataacg ggcaatcagg gagaactcgc cacatgccat acgttatgcc
     1741 tcagcaggta aaaaagaaaa ggccggcgat cgcggaacag tcttccgtga atctaccggc
     1801 ctggatatta ctttttgtta gggcgaatca caggtgctgc tttatcgagt acgccgttga
     1861 cgaacttatg gctgtcttct gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg
     1921 ccactttgta tggcacatcg ctacgtttag acagttcgta cagcgcaatg cgcagtactg
     1981 ctttttctac ctgtcccagt tcttccagca ggcgggacag gtatggcttc atcagtccgt
     2041 cgaggtatgc ggtattagtc gccaccccgg ccagcagctc acggaagtac aggacgtcaa
     2101 cgtcttttac atcctgttca gccaggaact ggtattcaac atcagcgatg tcgttctggg
     2161 acaactgcca ggagtagagc gcctggacgg cacactcacg agcgcggcga cgagcagcag
     2221 gtttcacgga tttcccctta ctaatttcag gccttgatgg ctttcaatac attaatcatt
     2281 tcaagcgcgg tcagtgcagc ttctgcacct ttgttgccag ctttggtgcc agcacgttcg
     2341 atcgcttgtt caatgctttc agtggtcaga accccaaaag caaccggaat ttcgctgtcc
     2401 tgggcaacat gcgccaggcc gttgcttgca ccaccagcga catattcaaa atgggcagtg
     2461 ccaccacgaa taaccgtacc cagcgcaatc accgcgtcgt atttaccggt tttagccagt
     2521 gcacccgccg ccagcggcag ctcataggca ccaggcaccc aaacaacggt aatgttttca
     2581 tcttttacct gaccgatacg tttcagtgca tcaattgcac cttccagcag gctgtcattg
     2641 ataaagttgt tgaaacgcgc gatggtgatg gcgacgcgag cgtccggggt agcaacgtta
     2701 gcttcaataa tgttcatttt cttccttcgg gttcgagtat ggccccgcag gggggcggat
     2761 tttagcataa tatttcgtgc gctgcttccc tttcgagccg ggagatcatg cacccactaa
     2821 atgcaggcaa acatccgggc ctacatgacg tatctctttg aatttaaatt ggggggcgtc
     2881 agctaatttc tcaagccctg gcagtgtgca taatccacgg gcgtcgctgc ctaatagttt
     2941 aggtgcgata tagacaatca gctcatcgac taaccccgcc tgcagcaatg cgccagcgag
     3001 cgttggcccc gcttccaccc agatgctgtt aatctgctgt ttacccagtt gcatcatcag
     3061 tacaaccaga tccagatgac ctttatgctc tggaatcagc aaggtacgca ccgtttccgg
     3121 ccactcacga gaatcttcct gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat
     3181 gtgatgttcc ggcgtcacgc gattttggct atcaatcaca atacgtaccg gctggcggag
     3241 atgatgttgc gggtagagcg cctgagtttg tacatccagt tcagaccaac gcaccgttaa
     3301 ggcaggatca tccgccagca ccgtagcgct gctggttaaa atggcatgac tttgtgcgcg
     3361 cagtcgttgt acgtcgcgcc gcgcctgagg cgaagtgatc cactggcttt cgccgctcgc
     3421 catcgccgtg cggccatcaa gcgatgcgcc aagttttaac tgaatataag gaaagccggt
     3481 gcgcatccgc ttgagaaagc ctttattcaa ttgctcggct tcactcatca tcaggccgtg
     3541 gctgacgtca atgccagcct gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt
     3601 cgggtcctgc atcgcagcga ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca
     3661 cggtggcgta cgaccatgat ggctacaggg ttcgagtgtg acataggcgg tcgcaccttt
     3721 ggctttttca cccgccatac gcaacgcgtg tacttcggca tgtggttcac ccgctcgttg
     3781 gtggtaacct tcaccgacaa tttcgccatc tttgacaatg acgcacccga cattcgggtt
     3841 ggggtgggtg gtaaaacgtc ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc
     3901 gtcctgcacg gcttagtcct ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc
     3961 gaaactgcgg tagacagagg caaaacggat ataggcgact ttatcgagct ttttcaattg
     4021 ctccatcacc agattgccaa tcatcttgct cggcacttcg cgctcaccgg tggcgcgcag
     4081 ctgcgattta atatgattga ttgccatttc gacgtcatcg gaactcaccg gacgtttttc
     4141 cagcgcccgc agcattccgc tacgtaattt ttcttcatta aacggttcac gcacgtcgtt
     4201 gctttttaca acacgcggca taaccagctc cgccacttca aaggtggtga aacgttcatt
     4261 acacaccaga cactgccgac ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc
     4321 aattacctta gtgtccacgg cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt
     4381 ggttaacgga aatctatttt accctgaact gacgatacaa caaaggcgca acgcttttga
     4441 gataaaggat cgatgctttc gctgctttta ccctctacca ttaggctaat tgcgacgaga
     4501 aagggacgac ctatgaatat taacgttttt cgactgatcc tgctgggaag cctattcagc
     4561 cttagcgcct gtgtgcagca aagtgaagtg cgacagatga aacacagcgt cagcacgctg
     4621 aaccaggaga tgacgcaact caatcaagaa acagtcaaaa tcactcagca aaacaggctg
     4681 aatgcaaaat ccagcagtgg ggtttacctt ctgcctggag cgaaaacacc ggcaagactg
     4741 gaaagccaga tcggtacttt acgtatgtcg ctggtgaata ttacgcctga tacagatggc
     4801 accaccctga cactacgtat tcagggtgag tcgaatgatc ccttgcccgc attcagcgga
     4861 actgttgaat atgggcaaat tcagggaaca atagacaact ttcaggaaat caatgtacag
     4921 aatcaattga ttaatgcccc tgccagcgtc ctcgccccca gcgatgttga tattccgtta
     4981 cagttaaagg ggatttctgt ggatcagtta ggctttgtac gtatccacga cattcaacct
     5041 gttatgcagt aaacttatag cggggcgatt ttgtgcgtcc cgcaacatcc ttccccatca
     5101 ttttgttact ccgcttacat aacccagatt gatagtaaaa gtttgcaaca agggcgaaag
     5161 tcagtacaat ccccgcccga atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata
     5221 gaactgtgaa acgaaacata tttttgtgag caatgatttt tataataggc tcctctgtat
     5281 acgaaatatt tagaaacgca atttgcgcct ttttcactcc cgcaagggat tttcaaacag
     5341 tggcatacat atgaaaaaaa cattactggc agccggtgcg gtactggcgc tctcttcgtc
     5401 ttttactgtc aacgcagctg aaaacgacaa accgcagtat ctttccgact ggtggcacca
     5461 gagcgttaac gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc gcaacgatac
     5521 ctaccttgag tacgaagcat tcgctaaaaa agactggttc gacttctatg gttatgcgga
     5581 tgcgccggta ttcttcggcg gtaactccga tgcaaaaggt atctggaacc acggttctcc
     5641 tctgtttatg gaaatcgaac cacgtttctc catcgacaag ctgaccaata ctgaccttag
     5701 cttcggtccg ttcaaagagt ggtacttcgc gaacaactac atttacgaca tgggtcgtaa
     5761 taaagatggt cgccagagca cctggtacat gggtctgggt accgatatcg acactggcct
     5821 gccgatgagc ctgtccatga acgtctatgc gaaataccag tggcagaact atggcgcagc
     5881 gaacgaaaac gagtgggacg gttaccgttt caaaattaaa tactttgtgc cgattaccga
     5941 tctgtggggc ggtcagctga gctacatcgg cttcaccaac ttcgactggg gttccgattt
     6001 aggggatgac agcggtaacg caatcaacgg tattaagacc cgtactaata actctatcgc
     6061 ttccagccat attctggctc tgaactacga tcactggcac tactctgtcg tagctcgtta
     6121 ctggcacgac ggtggtcagt ggaacgacga tgcagaactg aacttcggca acggcaactt
     6181 caacgttcgc tctaccggct ggggtggtta cctggtagta ggttacaact tctgattatg
     6241 aaaatgccgg gatttattcc cggcatttca gattgttaac aacgtacgct ttgttcatgc
     6301 cggatgcggc gtaaacgctt tgtccggccc acaaaagcac gtaaattcaa tatattgctg
     6361 aaataacgta ggcctgataa gcgtagcgca tcaggcaatt ttacgtttta atcacttctt
     6421 cttgttcatc atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt
     6481 ctgcgcatct tcccctgcga taacggtcgt accatactga tccgcttcgg tatatttcga
     6541 atgctcatga tcgtggcaat agagacaaag caattcccag ttactgccat cttccgggtt
     6601 attggtatgg tcgtgatcaa tgtggtgaac ggtaagttca cgcaggttgg aataaacaaa
     6661 ctcgcgggaa cagcgaccgc agacccacgg atagattttt aatgcttttt cgcgatagcc
     6721 gctttctaac cgcgcatagt tttttgggat gatagccatg aaaagttacc tggatgaaat
     6781 gtagggttac gcgcagtttc ccaagatgcg gggattttcg caatcctgag tcaagaaatt
     6841 ctcacattac cagtgacggc gaggttccat caactgatga agaatgcgcg caatgagaat
     6901 gtcatcgact cttataacgt aataaacagt gtgaaaccta aatgcgatct tctttacctc
     6961 tggaatcgca tcatagtctt gccctatctc gggcatttga gagagtgtat ccagaaaagt
     7021 gccgagagag tcggtatagc gatccgcctg ctcatcacca aactttccca gcgtgtagtc
     7081 atagatccct ctgaaatctt caacagcttt tccgctaagc ttatacacga tgtttttgct
     7141 tcctttctgc tgcgatatca cgaagcgtca tcgaactttc accgctctca agaccttcaa
     7201 taacagcatt tctgacagct tcatccctgg attcttgttg ttccaataga cgaagtgccg
     7261 aacgcattac ttcactggcc gagccataac gaccagactg gaccatccgg gtaataaaac
     7321 tatctaattg ttcgccaacg cttactgagg taattttgcg cataagcgat ctccacgtat
     7381 taactattaa tacaaagtat atgtcagaac gctttctaca accaaaccgt attcagttgc
     7441 ctgtgaaaca ggcaaaaaaa aaccgcagcg caaatacgcc acggtttttt taacagaacg
     7501 gatttaaaac accattacgg cagaattgac ggctgatccg ccccttcttt ttccactttc
     7561 tgctgcaaca tgtgttcgcg cttcataccc agtttcagag ccaacgcaga tgccacatag
     7621 atggaagatg cagtaccgat ggaaacaccg ataagcatgg tcagcgagaa gccttccagt
     7681 accggaccac cgaagagata cagcatcagg ataaccatca aggtagtacc ggatgtgatc
     7741 aaggtacggt gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga
     7801 cggatcttgc ggaagttttc acgaattcgg tccgatacca cgatactgtc gttaagcgag
     7861 taaccgataa ccgacatcaa cgatgccaca atggtcaggt caatctcgat atggaataac
     7921 gacaaaatac ccagcgtaat gataacgtcg tgcgccagcg caataaccac ccctgccgcc
     7981 agtcgccact caaagcggaa acctacgtac acgaggatag acagcagcgc tgccatcaac
     8041 gccatcgcac cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc
     8101 ttcactgctg cattctgatt ggtggattcg ttaatcacct tcagaacctg gctgcccagc
     8161 acctgaccgc cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta
     8221 ccaaagtttt gcagcatcgg ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg
     8281 tcaatttcag ccggtttttc gagcgtaatt tcgataaccg taccaccggt gaaatccagc
     8341 ccccagttaa agccgcgcac gcccataata acgatagcag cgattaacag cagaccagag
     8401 atgccaaaag cccagtagtc ccagcgcata aagtcataga ctttacggcc gtggtttagt
     8461 tgttcaacag tatattcctg tgccacatcg cactccttag attgacagct tcttgacgcg
     8521 cttgccgcca tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg taaacatcga
     8581 cgtcgccaca ccgataccgg tagtaatcgc gaacccttta attgccccgg tacccactgc
     8641 gtacaggatg atgactttaa tcagcgtggt gatgttcgca tcgaagatag aactgaatgc
     8701 gccacgataa ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca actcttcttt
     8761 aatacgttcg ttgatcagta cgttcgcatc gaccgctacc gcaagggtta agacgatacc
     8821 cgcgatacct ggcatactca gtgttgcgcc tggcaacagc gacataatac cgacgatcaa
     8881 gatcaagttg gcaatcagag cactggtcgc aatcagacca aacttcttat agaagatgat
     8941 catgaacaga atagaaacca gcagaccggc aaggcaagcc tccagcccct gctcaatgtt
     9001 ctgcataccc agggtcgggc caattgtacg ttcttcaaca atctgaatcg gcgcgatcaa
     9061 cgcaccggca cgcagcagca gtgacagctg acgggcttcg ttcgggttgt tgatgccggt
     9121 gatacggaag ctgttaccca gacgagactg gatgttggcg atgttaatca cctcttcctg
     9181 tttcaccaga accgcacgac catttgcatc tttcttaccg ctgtctttgt actccacaaa
     9241 cagggttgcc atcggtttgc cgatgttgtc cttggtgaag ttagacataa tgttgccacc
     9301 agcgctatcg agcgagatgt taacctgcgg ctggttgtat tcgtcctggc tggaagtgga
     9361 gtcggtgata tggtcaccgg taagaattac gcgtttgtac agcacaaccg gctgaccttc
     9421 gcgggtctgt ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag cggcctgatc
     9481 aacgttggtg tttaccagac ggaattccag cgttgccgtc gcacccagaa tctctttcgc
     9541 acgcgcagtg tcctgaatac ctggcagttc aacaacgata cggtcagcac cctgacgctg
     9601 aaccaccggc tccgccacgc caagttggtt tacacggtta cgcaggatat tgatgttctg
     9661 ctgcaccgca tattcacgcg cttcactcag acgagcatcg ctcattaccg cacgcagctg
     9721 gttgctgccc tgactgctaa tcaccaggtc cggatgacgc ttgctcagat acgcaatggc
     9781 ttcatcacga gctttagcat cgcggaaagt gatgctcagg ccgtagttat tttctttacg
     9841 aacggtggta tacgggatgc ctttttcgcg caggtcgctg cgcaggctat cgatattttg
     9901 ttcctggagt ttgccaagcg cggtgtccat atccacttcc atcaggaagt gaacgccgcc
     9961 acgcaggtca aggccgagct tcatcggctc agcgtgaata gctgccagcc agcgcggcgt
    10021 tgccggggca aggttaagcg ccacgacgta tttgtcaccc ataacgccca ttaatgcttc
    10081 acgagcgcgc agctgggtgt cagtggagtc gaagcgcgca agaatagcgc cctcttccag
    10141 tgccacagac ttagcagtta ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt
    10201 ttgctcactg gcggcgactc cgcgcgcacc agtgatctga acagccggat cctcaccaaa
    10261 caggttggga agcgcataca gcagaccgat gacaatcacc acgatcagca tgacgtactt
    10321 ccacaaagga taacggttta acacggcaat tcccttaggg aaaaatttta attacagcgc
    10381 cttcatggtg cctttcggca ggacggcagc tacgaagtca cgtttaataa ctacttcagt
    10441 ggtgtcattc agcgcgatag caatgtagcc gttttccgct actttggtta cgcgaccaac
    10501 caggccaccg ttcgtcagaa cttcatcacc tttggcaatg gagtccatca gctttttgtg
    10561 ttctttggtg cgcttctgct gtggacgcag gatcatgaaa tagaaaatca gaccgaatac
    10621 caccagcatc aaaatcaaag acatcgggct accttgcgcc ggtgcacccg ttgccgctac
    10681 cgcatcagaa ataaaaaagc tcattaaatt tccctcatta ttaatattaa tcaacgttca
    10741 aaggtggtac ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc tctaatttac
    10801 cctcttcaat agccttgcgt aaacccgcca tcaaacgctg gtagtaacga aggttatgaa
    10861 tggtattgag tcgcgcgcct aatatttcgt tgcaacggtc aagatgatgc aagtaagcgc
    10921 gtgaataatt gcgacaggtg tagcaatcac actcaggatc gagtgggcca gtatcgctct
    10981 tatacttcgc attgcggatt ttcaccacgc catcggtcac gaacaaatga ccatttcggg
    11041 cgttgcgggt tggcattacg cagtcaaaca tatcgatacc acgacgtacg ccttcaacca
    11101 ggtcttctgg tttaccaacg cccatcaggt aacgcggttt gtctgccgga atttgcgggc
    11161 atacatgctc cagaatgcgg tgcatatctg ctttcggctc acccacagcc agaccgccga
    11221 cagcgtagcc atcaaaaccg atatctacca gacctttaac agaaatatca cgtaaatctt
    11281 cgtaaacgct gccctgaatg ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac
    11341 gctcacggct acgcttcgcc caacgcagag acatctccat ggagcgtttt gcgtaatccc
    11401 agtcagcagg atacggcgta cactcatcaa agatcatgac gatatccgaa ccaagatcgt
    11461 actgaatctc catcgacttt tccgggtcga ggaaaatcgg gtcgccgttg atcgggttac
    11521 ggaagtgcac gccctgttcg gtgattttac gaatatcgcc aaggctgaag acttggaagc
    11581 cgccggagtc ggtgaggatc ggtcccttcc actgcataaa atcgtgcaga tcgccgtgca
    11641 gtttcatgat ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg aggataattt
    11701 gcgcgccagt ggcttcaact tcttccggcg tcatcccttt tacggtgccg taggtgccaa
    11761 caggcataaa acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg
    11821 cgcgaccgtc ggtggtgtcc agttcaaatt tcattttttc tccactacgt cagaaaaaca
    11881 gtccaacgtt taaaccagcg ccgcggaatt actccccgac gcgctcatta attgcctgcg
    11941 gattgtacgt gataaacatc gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg
    12001 ccgctttata ggcattcatt gtgtgttgat aaccggcaaa ggccgaaacc agcataatca
    12061 gcgtcgattc cggaaggtgg aagtttgtca ccagcgcatc gaccactttg tactggaagc
    12121 ccggatagat aaagatttgg gtatcgtcaa agaacggttc gatgagatcg tttttcgccg
    12181 cctgagccgc gctttccagc gaacgtactg aggtggtgcc aaccgcaatc acccggttac
    12241 cgcgcgcttt cgccgccagt accgcgtcta ccacatcctg cggtacttca gcgtattccg
    12301 agtgcatgat gtgatcttca atggtgtcga cgcgtaccgg ctggaaggtg cccgcaccaa
    12361 cgtgcagggt gacgaacgcc atctccacgc ctttggcgcg taatttttcc agcaaaggtt
    12421 cgtcaaaatg cagaccagcg gtcggggctg caaccgcgcc cggtttttcg ctataaacgg
    12481 tttgataaag ttcgcggtca gcgtcttcgt ccggacggtc gatatacggc ggcagcggca
    12541 tatggccgat gctgttgaga atatccagca ccgagcgttc atcattaaat tcgacttcaa
    12601 acagtgcgcc gtggcgcgcg gtcattgttg cgttaatact ttcgtcatcg cccagcagca
    12661 gttctgcgcc aggttttggc gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt
    12721 cgagcatccg ttcaaccagc acttcaatct tgccgccgct ggctttacgc ccaaacaggc
    12781 gcgccgggat cacccgggta ttattaaaaa ccagaagatc gccggggttg agcttatcga
    12841 gtaaatcggt gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac
    12901 gacagctact gcgttcaggc atcggatagt gggcaatcag ggattcgggc aattcaaagg
    12961 agaaatcggt aacgcgcatg acactgactc agactaaaat aagaggcggg tagtctagtg
    13021 ccggggcgct ctccctgcaa cctttacccc cctttggata gaatataatc gatgaatttt
    13081 ttagctcacc tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa tttgctggct
    13141 gatttcgtac gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc tggcattcat
    13201 atgcatcgac gtatcgacgt attgactgac aatctgccgg aagtccgcga agcacgggag
    13261 tggtttcgta gtgaaactcg ccgcgttgcg cctattacgc tggatgtcat gtgggatcac
    13321 tttctttccc gtcactggtc gcagctgtcg ccggactttc cgctacagga atttgtttgt
    13381 tatgcccgcg agcaagtgat gacaattttg ccggactcac cgccacgctt tatcaacctg
    13441 aacaactact tatggtcaga gcagtggctg gtgcgctatc gcgatatgga tttcatccag
    13501 aacgtgttaa acggcatggc aagccgtcgc ccacgtctgg atgccctgcg tgactcctgg
    13561 tacgatttag acgcccatta tgccgccctc gaaacccgct tctggcagtt ttatccacgg
    13621 atgatggcgc aggcgtcacg caaggcgctc tgaaacttca aactccggcg tcactatttt
    13681 ttagcattcg catccagcgg ggcggcataa caacggtaag aaaaacattc gctggaatga
    13741 ttggcgatat ctttaaagtt gctttcagca accccctcga acatgagtac cgttctggac
    13801 aactctccgt tttcatttat atgtggaaaa tcaaattggc tactgaatcg ccatacatct
    13861 gtcttttctg gctcgctctt cacagagata tgtcgatgga ggacccctcc tgtaaacgtg
    13921 cgccagaaca attgttgatc aaaatcttgt aatatcgcag acttggatgc tatttgacgt
    13981 agtaaacctg cataccgtgg actgccatta cgagcaacat tatgcaaacc ctgactaaaa
    14041 gcatcaaaca aacaatagat gtgatcacta gtgaaaaaag gtcttaattt tttttgttct
    14101 tcatcgctgt aatccaggag catctcattg attt
//
LOCUS       DAZKFZ010000110        13912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000110 DAZKFZ010000000
VERSION     DAZKFZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1907)
                     /locus_tag="ACHOQW_002969"
     CDS             complement(<1..1907)
                     /locus_tag="ACHOQW_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFP9574015.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAV"
     gene            complement(1998..2082)
                     /locus_tag="ACHOQW_002970"
     tRNA            complement(1998..2082)
                     /locus_tag="ACHOQW_002970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2046..2048),aa:Leu,seq:caa)
     gene            2278..3297
                     /gene="ahr"
                     /locus_tag="ACHOQW_002971"
     CDS             2278..3297
                     /gene="ahr"
                     /locus_tag="ACHOQW_002971"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP9574016.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3427..4929
                     /gene="yjgR"
                     /locus_tag="ACHOQW_002972"
     CDS             3427..4929
                     /gene="yjgR"
                     /locus_tag="ACHOQW_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP9574017.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5090..6172)
                     /gene="lptG"
                     /locus_tag="ACHOQW_002973"
     CDS             complement(5090..6172)
                     /gene="lptG"
                     /locus_tag="ACHOQW_002973"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9574018.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6172..7272)
                     /gene="lptF"
                     /locus_tag="ACHOQW_002974"
     CDS             complement(6172..7272)
                     /gene="lptF"
                     /locus_tag="ACHOQW_002974"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9574019.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7335..7385)
                     /gene="ytgA"
                     /locus_tag="ACHOQW_002975"
     CDS             complement(7335..7385)
                     /gene="ytgA"
                     /locus_tag="ACHOQW_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP9574020.1"
                     /translation="MSIIILATISTTQENK"
     gene            7539..9050
                     /gene="pepA"
                     /locus_tag="ACHOQW_002976"
     CDS             7539..9050
                     /gene="pepA"
                     /locus_tag="ACHOQW_002976"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP9574021.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9404..9847
                     /gene="holC"
                     /locus_tag="ACHOQW_002977"
     CDS             9404..9847
                     /gene="holC"
                     /locus_tag="ACHOQW_002977"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP9574022.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9847..12702
                     /gene="valS"
                     /locus_tag="ACHOQW_002978"
     CDS             9847..12702
                     /gene="valS"
                     /locus_tag="ACHOQW_002978"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9574023.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12757..>13912)
                     /gene="yjgN"
                     /locus_tag="ACHOQW_002979"
     CDS             complement(12757..>13912)
                     /gene="yjgN"
                     /locus_tag="ACHOQW_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP9574024.1"
                     /translation="FVFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYANM
                     EVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCVLTLSLLVLLIFMAA
                     KGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTVFFISTKMLHANSSS
                     SVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAIL
                     AFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGI
                     AVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPL
                     LKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         3582 a         3566 c         3603 g         3161 t
ORIGIN      
        1 acagcatcca gcccatcagg aatgttgaat ctcgctatct cgggcaaaga aaaatcaatc
       61 ttttcgacaa cctcaaccat gtgaaccggt tttaaatctt tgctgtatcg cacttcagtt
      121 tctgttttgc ctcgcacatg cccaagtagc tgcccacgca gttccgaact aacacccttc
      181 tctttaagca aagttgaaac agaatgccgg aaagagtgga aagatttgct tctgtctctt
      241 tcaagtttta atcgacccgc aagcaatgat tcattgaacc atgcggaaac cggcctacca
      301 tagcctttgg ttttatgtgg tttaagctcg ggaaaaagac ggtcataccc ggcagctttc
      361 aacgcatcca catactcgat aaaaccgagt tcaatcagcg tagaatgaac cggaatcttg
      421 cggcgggaat tggcgttttt tagtttcttt ccgttttcgc tatcgctttc aaggttcagg
      481 taataaacgc cgtcatctgc cagaacatcg tccagcatta actgtgcaat ctcgttaacc
      541 cttgcccccg tcatcaagcc caacaacggc gcccaatagt gaaacgggca atattgatgg
      601 aatcgccctt gcgcattacg ttcaacagtt ccagcgacaa accacggttg cgaaaaaatt
      661 gccttaattt catgcggttc aaatatgtca gtgtgatcct gctctcgctc acctttgtca
      721 tcgggaggga aaaagttgtc gcatggcgat ttcagaaact taccatcatc aacagcccac
      781 tccattgcac cataaaggcc gttgatatac tttttgacag actcttctgt catcagttta
      841 tcgccacatt ctgccgcttt ggcgattaac tcatccagcg tcttaacccc gtgcttacct
      901 ttcatcaaat tacggttagc aggaattgtg cgcaacttgc tctcataagc ccggaggaaa
      961 tcacggtcga aatcttccaa ctgaatatcc cccatcactt caatcatcaa atcgaagtaa
     1021 tagcgaatat cgcggagcgc cgcttctgat accttgccct tacgtcgatg tttcaaatac
     1081 tcttcacgaa gccatgacaa gttttcattg taatgtttgc ggtgaacata ttcattagcg
     1141 ataacaggag cgacaagagg cacggcaggg tgaatgctaa ctgctggtgg cgtggtctta
     1201 accagccatg taagtcgaag gccttcagca tgaactttgc taatcaacag gctatcagca
     1261 gttatatcta cgccgggtaa atcgaagaac caacctccac tttggttatc accactagtt
     1321 cgaaatgcag cctctccttc ggatactcca gcgctcaaca gacaatctaa agtatgccgg
     1381 ggattgaagg ggcggagata accatttgcg aagctctcga tacccagctc aaacgcgcta
     1441 ttgagaatga tgacttcttt aggttgatcc ttttcatcat cagactgttt ttctcgtttt
     1501 gcacccggtt cccgctctac tgcattaaat tcatcaacat agaaaccgaa ctgaccagtc
     1561 gccagccaga ataccgggag gttacgatta accacctctc gaatcaattg cgatataggc
     1621 aactcgattg actgagcaaa gttagaaagc gagatcatac cctcaccagc aagcagtggg
     1681 gcgcagtcac ttaaagttcg ataatccaat tgttcatact cttgtaaggt ctgataccag
     1741 accgccaaca ggagcgcgga cttctgcttt gcttcatgaa gatcgtgagt gcctgttgaa
     1801 gtgtgaatct cacactgtcc tgcgtatcga cgaaaacgtt ggggaacgct aatgcggaca
     1861 acataaatgc cgctggttcg acgatagagt ttatatcgct gagacatcgc ctcgtatacc
     1921 cttttgtaag aggggctacg cgatttcaag caagattatg aattaaattg ttttaaaaca
     1981 atagtttaga tgagtcttgg tgccgaaggc cggactcgaa ccggcacgta tttctacggt
     2041 tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga ggtatgcgga
     2101 aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg ctcgcacctc
     2161 gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt cacactccag
     2221 gcaccccgcc ctgccatgct ctacacttcc caaacaacac cagagaagga ccaaaaaatg
     2281 tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt ttatgagtac
     2341 gatcccggtg agctgaggcc acaagatgtt gaagtgcagg tggattattg cgggatctgc
     2401 cattccgatc tatcgatgat cgacaatgaa tggggatttt cacaatatcc gctggttgcc
     2461 gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga taaaggtttg
     2521 caggtcggcc agcgtgtcgg gattggctgg acggcgcgta gctgtggtca ctgcgacgcc
     2581 tgtattagcg gtaatcagat caactgcgag caaggtgctg tgccgacgat tatgaatcgc
     2641 ggtggctttg ccgagaagtt gcgtgcggac tggcaatggg tgattccact gccagaaaat
     2701 attgatatcg agtccgccgg gccgctgttg tgcggcggta tcacggtctt taaaccactg
     2761 ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg cgggctgggg
     2821 catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacggcctt tagttctaat
     2881 ccggcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa tagccgcgat
     2941 ccgcaggcac tgaaagccct gtcggggcag tttgatctca ttatcaatac tgtgaacgtc
     3001 agcctcgact ggcagcctta ttttgaggcg ctgacctatg gcggtaattt ccatacggtc
     3061 ggtgcggttc tcacgccgtt gccggttccg gcctttacgc tgattgcggg cgatcgcagt
     3121 gtctctggct ctgctaccgg tacaccttat gagttgcgta agctgatgcg ctttgccgcc
     3181 cgcagcaagg ttgcgccgac taccgaattg ttcccgatgt cgaaaattaa cgatgccatc
     3241 aagcatgtgc gcgacggtaa ggcgcgttac cgcgtggttc tgaaagctga cttctgatct
     3301 ctttgccggg tgcctgtcgc ccggcactta aatctctttg gaaggcgtct cgcaatctgc
     3361 tgaatttagc ggtgaagagt gcgcagccgt tgcctatact ccaggcagga aactggagga
     3421 tatctcatga gcgaacccct gttaattgcc cgcacgccgg acacagaact atttttactg
     3481 ccgggaatgg ctaaccgtca cgggctgatt actggcgcaa cggggacggg taaaactgtc
     3541 acgctgcaaa aactggcgga gtcattgtcg gaaatcggcg tgccggtgtt tatggctgat
     3601 gtgaaaggcg atctgaccgg tatcgcgcag gcaggaacgg cgtcggaaaa actgctcgca
     3661 aggcttaaaa atatcgacgt caatgactgg caaccgcatg ctaatccggt ggtggtgtgg
     3721 gatatctttg gcgagaaagg ccattcggta cgggcgacgg tttcagacct ggggccactg
     3781 ttgctggcgc ggctgttgaa tctcaacgat gtgcaatctg gcgtgctgaa tatcatcttc
     3841 cgcattgctg acgatcaggg gctgttactg ctcgacttta aagatctgcg ggcgattacc
     3901 cagtacatcg gcgataacgc caaatctttc cagaatcagt acggaaatat cagtagcgca
     3961 tcggttggtg ccatccagcg cgggctgttg tcgctggaac agcaaggcgc agcacacttc
     4021 tttggtgagc cgatgctgga tatcaaagac tggatgcgca ccgatgccaa cggtaaaggc
     4081 gttatcaata tcctcagcgc cgagaaactt tatcagatgc cgaaactgta cgccgccagc
     4141 ctgctgtgga tgctctcaga gttgtatgaa caattgccgg aagcgggcga tctggagaaa
     4201 ccaaaactgg tgtttttctt cgacgaagca catctgctgt ttaacgatgc accgcaggta
     4261 ctgctggata agattgagca ggtgataagg cttattcgct caaaaggcgt gggcgtctgg
     4321 ttcgtttcgc aaaacccgtc tgatattccg gataatgtgc tcggccagct cggtaatcgc
     4381 gttcagcacg ctttgcgggc ttttacgccc aaagatcaga aagcagtaaa agctgcggcg
     4441 caaaccatgc gggccaatcc ggcgtttgat accgaaaagg cgattcagga actgggcacc
     4501 ggcgaggcgt tgatctcttt tctggatgcg aaaggaagcc cttctgtggt ggagcgtgcg
     4561 atggtgatcg cgccttgttc gcggatgggg ccggtgacgg aagatgagcg taatggcttg
     4621 attaatcact ctccggtgta tggcaaatat gaggatgagg tggaccggga atccgcctat
     4681 gagatgttgc aaaaaggctt tcaggccagt accgagcagc aaaataatcc ccccgcgaaa
     4741 gggaaagagg tagcggtgga tgacggcatt cttggtggat tgaaggatat tttgttcggc
     4801 actaccggac cacgcggcgg gaagaaagat ggtgtggtgc aaacaatggc caaaagcgcc
     4861 gctcgccaag tgacgaatca gattgtacgc gggatgttgg ggagtttgct gggggggaga
     4921 agaaggtaag tcaggcgaga tttgtctttg cctgatgcga cgctggcgcg tcttatcatg
     4981 cccaccccac tgcaatatat tgaattttaa ttatttttca ggaatgaacg aagcacactt
     5041 tgctaacaat aataaaggga gctttcgctc cctttattcg ttcattcggt tacgattttc
     5101 tcattaacag ccacaggctg attaagaaga agctggcgct tggcaacagt gcgccgatga
     5161 tcggcgggat gccataaacc aacgtcagcg ggccgaagat ctggtccagt acgtagaaga
     5221 caaaaccgaa actgataccg gtgaccacac gcacgcccat cggtacgcta cgcagtgggc
     5281 caaagatgaa cgacagcgcc atcagcatca tcaccgccac ggatagcggc tggaagattt
     5341 tgctccacat gttgagctga taacgtccgg catcctgacc gctcgacttc agatacttca
     5401 catagttgtg caaaccgctg atagagagtg catccgggtc cagcgccacc acgcccagtt
     5461 tgtccggcgt gaggttggtt ttccaggtgc cgctcaccgt ctgcgaaccg gtaatctgtt
     5521 tcggattggt cagatcagat tcatcaacct gcgacaaacg ccagacttta tgttccgggt
     5581 caaacttcgc agtagcggca tagcgtacgg attgcagacg acgattctcg ttaaaggcat
     5641 aaatgctgat gccacctaac tcttcgtcac ctttaacccg ctcaatgtag acgaagttgt
     5701 tgccatcttt cgcccataag ccttgctggg tagagagcaa cgagccgccg tacatcgcct
     5761 gcgcacggta gttacgcgcc atctgctcgc cctgcggcgc gacccattcg ccaatcgcca
     5821 tcgtcagcaa gaccagcgga atggcggttt tcatcaccga cagcgccacc tgcatacggg
     5881 taaaaccaga agcctgcatc accaccagtt cgctgcgctg cgccagcatc ccaagaccaa
     5941 gcaacgcccc aagcagagcc gccatcggga agaatatttg cacatctttc ggcacgctca
     6001 gcaaggtata cattcctgcg cctaacgcgt cgtaactccc ctgcccggct tttttcaact
     6061 gatcgacaaa cttgataatg cccgacagcg acaccagcat gaacagcgtc atcatgatgg
     6121 tggtgaaaat agttttaccg atatagcggt caagtacgcc aaaaggttgc atcacaccgc
     6181 tcctttacgc gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag
     6241 aacaatcgcc aaagccagat aaatcaggtt aacggtccac atccacagcg tcgggtccag
     6301 cttaccttta ccgccgttcg atttcaggga ggtctggatc aggaagaaaa gtagatacag
     6361 cagcatggct ggcagcatcg acagtacgcg tccctgacgt gggttaacca cgctcagcgg
     6421 tacgaccata agtgccatca taaacacggt gaataccaac gtgatacgcc agttcagttc
     6481 tgcgcgagca cgatcggtgt cagtgttcca caatgtgcgc atgtccatct ggtcggtatc
     6541 gttcgggtcg agcgccaccg cctggtgacc aatgatcgcc tgataatcct ggaaatccgt
     6601 aatgcggaaa tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac
     6661 gacctgggag ccgtcgcgca gctgggttaa atgtccggaa tcggccacca ccacagaagg
     6721 acgtgcatta ccttttggtc gaatttgcgc gaggaacaca tctttgaaat cgctgccgtc
     6781 aacgctttcg atgaacagca ccgaactgcc attagtcgct tgctggaatt gcccttgcgc
     6841 cagcgccgcc atgccagggt tcgctttcgc ttctgctaac acttcatcct gatgacgcga
     6901 tgaccacggt cccgcccaca tcacgttaac cgccgcgacg attgccgtga ataccgcaag
     6961 gatcattgcc gctttcacca gaaccgcttt gctcaggccg caggcatgca ttaccgtaat
     7021 ttcactttcg gtatacagtt tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg
     7081 caggataagc tgcgccattt ccggcacgcc caacccgaga agggagagca ccagattcgc
     7141 cggaatatcg ccgtcaaccg ctgcgccgag gatcctcact aacttttgac agaagaagat
     7201 caaaagcaag atgaagagta tcgccagctg gcttttgagc gtctcccgca ccagatatct
     7261 tatgattatc actttaaata cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt
     7321 tcatggctta aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa gataattata
     7381 ctcaacggat tcacctctca gattttgttc tgacgtgcca atgccgtaat aacgttaaga
     7441 ttaacacgaa gtcatcgcaa cagcggacat gagttacgaa agcttgcaat tctatctgta
     7501 gccaccgccg ttgtctttaa gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg
     7561 gtagcccgga gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc
     7621 tttctccgat tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc gccctgctac
     7681 gtcggggcga actggaagga aaaccggggc agacattgtt gctgcaccat gttccgaatg
     7741 tactttccga gcgaattctc cttattggtt gcggcaaaga acgtgagctg gatgagcgtc
     7801 agtacaagca ggttattcag aaaaccatta atacgctgaa tgatactggc tcaatggaag
     7861 cggtctgctt tctgactgaa ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc
     7921 aggctgtcga gacggcaaaa gagacgctct acagtttcga tcagctgaaa acgaacaaga
     7981 gcgaaccgcg tcgtccgctg cgtaagatgg tgttcaacgt gccgacccgc cgtgaactga
     8041 ccagcggtga gcgcgcgatc cagcacggtc tggcgattgc cgccgggatt aaagccgcga
     8101 aagatctcgg taatatgccg ccgaatatct gtaacgccgc ttacctcgct tcacaagcgc
     8161 gccagctggc tgacagctac agcaagaatg ttatcacccg cgttatcggc gaacagcaga
     8221 tgaaagagct ggggatgcat tcctatctgg cggtcggtca gggttcgcaa aacgaatcgc
     8281 tgatgtcggt gattgagtac aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg
     8341 tgggtaaagg tttaaccttc gactccggcg gtatctcgat caagccttca gaaggcatgg
     8401 atgagatgaa gtacgatatg tgcggtgcgg cagcggttta cggcgtgatg cggatggtcg
     8461 cggagctaca actgccgatt aacgttatcg gcgtgttggc aggctgcgaa aacatgcctg
     8521 gcggacgagc ctatcgtccg ggcgatgtgt taaccaccat gtccggtcaa accgttgaag
     8581 tgctgaatac cgacgctgaa ggccgcctgg tactgtgcga cgtgttaact tacgttgagc
     8641 gttttgagcc ggaagcggtg attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc
     8701 tgggtcatca cattaccggt ctgatggcga accataatcc gctggcccat gaactgattg
     8761 ccgcgtctga acaatccggt gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg
     8821 aacaactgga gtccaatttt gccgatatgg cgaacattgg cggtcgtcct ggtggggcga
     8881 ttaccgcagg ttgcttcctg tcacgcttta cccgtaagta caactgggcg cacctggata
     8941 ttgcaggaac cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgt ccggtagcgt
     9001 tgctggcaca gttcctgttg aaccgcgctg ggtttaacgg cgaagagtaa ttgcgtcagg
     9061 caaggctgtt attgccggat gcggcgtgaa cgccttatcc gacctacaca gcactgaact
     9121 cgtaggcctg ataagacgcg caagcgtcgc atcaggcaat gctgttattg tcggatgcgg
     9181 catgaacgcc ttatccgacc tacacagcac cgaactcgta ggcctgataa gacgcggcag
     9241 cgtcgcatca ggcaatgctg ttattgtcgg atgcggcgtg agcgccttat ccgacctaca
     9301 cacagcactg aactcgtagg cctgataaga cacaacagcg tcgcatcagg cgctgcggtg
     9361 tatacctgat gcgtatttaa atccaccaca agaagcccca tatatgaaaa acgcgacgtt
     9421 ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc aactggtgtg
     9481 tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgtg ctcatcgcct gtgaagatga
     9541 aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa gctttgttcc
     9601 gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg cctggccgca
     9661 aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct ttgcagattt
     9721 tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc tgaaacaact
     9781 ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata cggcaacctg
     9841 gaaataatgg aaaagacata taacccacaa gatatcgaac agccgcttta cgagcactgg
     9901 gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt ctgcatcatg
     9961 atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca gcaaaccatc
    10021 atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg gcaggtcggt
    10081 actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc cgcagaagaa
    10141 ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg ggaatggaaa
    10201 gcggaatccg gcggcacgat tacccgtcag atgcgccgtc tcggcaactc cgtcgactgg
    10261 gaacgtgaac gcttcacgat ggacgaaggc ctgtccaatg cggtgaaaga agttttcgtt
    10321 cgtctatata aagaagacct gatttaccgt ggcaaacgcc tggtaaactg ggacccgaaa
    10381 ctgcgcaccg ctatctctga cctggaagtg gaaaaccgcg aatcgaaagg ttcgatgtgg
    10441 cacatccgct atccgctggc tgacggtgcg aaaaccgcag acggtaaaga ttatctggtg
    10501 gtcgcgacta cccgtccgga aaccctgctg ggcgatactg gcgtagccgt taacccggaa
    10561 gatccgcgtt acaaagatct gattggcaaa tatgtcattc tgccgctggt taaccgtcgt
    10621 attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg cgtgaaaatc
    10681 actccggcgc acgactttaa cgactatgaa gtgggtaaac gtcacgcgct gccgatgatc
    10741 aacatcctga cctttgacgg cgatatccgt gaaagcgccc aggtgttcga taccaaaggt
    10801 aacgaatctg acgtttattc cagcgaaatc ccggcagagt tccagaaact cgagcgtttt
    10861 gctgcacgta aagcggtcgt tgctgcggtt gacgcactcg gcctgctgga agaaattaaa
    10921 ccgcacgacc tgacagttcc ttacggcgac cgtggcggcg tagttatcga accaatgctg
    10981 accgaccagt ggtacgtacg tgccgatgta ctggcgaagc cggcagttga agcggttgag
    11041 aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc ctggatgcgc
    11101 gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat cccggcatgg
    11161 tacgacgaag cgggtaacgt ttatgttggc cgcaacgaag aagaagtgcg caaggaaaat
    11221 aacctcggtg ccgacgttgc cctgcgtcag gacgaagacg ttctcgacac ctggttctct
    11281 tctgcgctgt ggaccttctc taccctaggc tggccggaaa acaccgacgc actgcgccag
    11341 ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg gattgcccgc
    11401 atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca ggtgccgttc
    11461 cacaccgttt acatgaccgg tctgattcgt gacgacgaag gccagaagat gtccaaatcc
    11521 aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc agaactgctg
    11581 gaaaaacgta ccggcaatat gatgcagccg cagctggcgg acaaaatccg taagcgcacc
    11641 gagaagcagt tcccgaacgg tattgagccg cacggcaccg atgccctgcg cttcaccctg
    11701 gcggcactgg cgtctaccgg tcgtgacatc aactgggata tgaagcgtct ggaaggctac
    11761 cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa cacggaaggt
    11821 caggattgcg gcttcaacgg cggcgaaatg acgctgtcgc tggcggaccg ctggattctg
    11881 gcggagttca accagaccat caaagcgtac cgcgaagcgc tggacagctt ccgcttcgat
    11941 atcgccgcag gcattctgta tgagtttacc tggaaccagt tctgtgactg gtatctcgag
    12001 ctgaccaagc cggtaatgaa cggtggcacc gaagcagaac tgcgcggtac tcgccatacg
    12061 ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc gttcatcacc
    12121 gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac catcatgctg
    12181 cagccgttcc cgcagtacga tgcatctcag gttgatgaag ccgcactggc cgacaccgaa
    12241 tggctgaaac aggcgatcgt tgcggtacgt aacatccgtg cagaaatgaa catcgcgccg
    12301 ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg tcgcgtaaat
    12361 gaaaaccgtg gcttcctgca aaccctggcg cgtctggaaa gcatcaccgt gctgcctgcc
    12421 gatgacaaag gtccggtttc cgttacgaag atcatcgacg gcgcagagct gctgatcccg
    12481 atggctggcc tcatcaacaa agaagatgag ctggcgcgtc tggcgaaaga agtggcgaag
    12541 atcgaaggcg aaatcagccg tatcgagaac aagctggcga atgaaggctt tgtcgcccgc
    12601 gcaccggaag cggtcatcgc gaaagagcgt gagaagctgg aaggctatgc ggaagcgaaa
    12661 gcgaagctga ttgaacagca ggctgttatc gccgcgctgt aatcaaaaca cagtgatgaa
    12721 aacgaaggcc ggagcatgct ccggcctttt tatctcttac agaaatggta aagaaggcat
    12781 aattccccgt gaaatactgg ctaagaagcc tttatctggt tgttcttctt tattgattaa
    12841 aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt caatcatcca
    12901 tattttcagc agtggataag ccagaccgcc cgtaatcccg gatatcacca ccaacgcaca
    12961 catgcgataa agcataccgt ggtatgttaa agttgagcga aaacgaatac gcccatcatt
    13021 cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac tggtgctaac
    13081 agcaatccca aaataataga ttaactgcgc gattatcatt ttacgttgca tttccataaa
    13141 ttgctgtaaa ttctcaatat catcatttgc atacacagaa ctatcatacg catttaatat
    13201 ttgatcgaag ataatataac cagcaacagc gagaaaaggt aataaagcta aaaatgcgag
    13261 gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat gtataccgaa
    13321 actggtattg ctccagagaa aactcattac caggctatat aaagtaccat taaaaatacc
    13381 aatggaaaca ataccaacta ttgccatcag aaccacagat acaataacac tacttgaact
    13441 attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa tggccattaa
    13501 aatcggcaag aaaaaggtta cccaccagac ccctttcata gagcaattaa aactaaatct
    13561 tacgccgttg agactggtca tcaaggcctg ataacgcagt ccttttgctg ccataaatat
    13621 aagcaaaacc aacagtgaca aagtcaaaac acagccaata agcggcatat ctgctgacac
    13681 tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaaaaa aaacattccc
    13741 accagtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat agagataacg
    13801 cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg ttaacaacac
    13861 attcaccaca caaataagaa aatatctctc acctgtacca tgaaaaacaa ac
//
LOCUS       DAZKFZ010000111        13746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000111 DAZKFZ010000000
VERSION     DAZKFZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            203..1312
                     /gene="livK"
                     /locus_tag="ACHOQW_002980"
     CDS             203..1312
                     /gene="livK"
                     /locus_tag="ACHOQW_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP9574025.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1360..2286
                     /gene="livH"
                     /locus_tag="ACHOQW_002981"
     CDS             1360..2286
                     /gene="livH"
                     /locus_tag="ACHOQW_002981"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP9574026.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2283..3560
                     /gene="livM"
                     /locus_tag="ACHOQW_002982"
     CDS             2283..3560
                     /gene="livM"
                     /locus_tag="ACHOQW_002982"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP9574027.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3557..4324
                     /gene="livG"
                     /locus_tag="ACHOQW_002983"
     CDS             3557..4324
                     /gene="livG"
                     /locus_tag="ACHOQW_002983"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP9574028.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4326..5039
                     /gene="livF"
                     /locus_tag="ACHOQW_002984"
     CDS             4326..5039
                     /gene="livF"
                     /locus_tag="ACHOQW_002984"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP9574029.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5158..5904
                     /locus_tag="ACHOQW_002985"
     CDS             5158..5904
                     /locus_tag="ACHOQW_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9574030.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6264..7580
                     /gene="ugpB"
                     /locus_tag="ACHOQW_002986"
     CDS             6264..7580
                     /gene="ugpB"
                     /locus_tag="ACHOQW_002986"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP9574031.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7678..8565
                     /gene="ugpA"
                     /locus_tag="ACHOQW_002987"
     CDS             7678..8565
                     /gene="ugpA"
                     /locus_tag="ACHOQW_002987"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP9574032.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            8562..9407
                     /gene="ugpE"
                     /locus_tag="ACHOQW_002988"
     CDS             8562..9407
                     /gene="ugpE"
                     /locus_tag="ACHOQW_002988"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP9574033.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9409..10479
                     /gene="ugpC"
                     /locus_tag="ACHOQW_002989"
     CDS             9409..10479
                     /gene="ugpC"
                     /locus_tag="ACHOQW_002989"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP9574034.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10476..11219
                     /gene="ugpQ"
                     /locus_tag="ACHOQW_002990"
     CDS             10476..11219
                     /gene="ugpQ"
                     /locus_tag="ACHOQW_002990"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9574035.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11206..11646)
                     /locus_tag="ACHOQW_002991"
     CDS             complement(11206..11646)
                     /locus_tag="ACHOQW_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP9574036.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            11766..13511
                     /gene="ggt"
                     /locus_tag="ACHOQW_002992"
     CDS             11766..13511
                     /gene="ggt"
                     /locus_tag="ACHOQW_002992"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP9574037.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT         3064 a         3476 c         3919 g         3287 t
ORIGIN      
        1 tctgtttatc tatcaataaa ttcagaatat tatctgttct taatcgactg aaaaataggg
       61 actttaatcg ctattatcac aaaatactgc gctaaaccct taatcagaca ggcaaaaaca
      121 gtgcagtata aaaaaagaac agtctgattt gttaacacat aaaaacaaag caacacaaca
      181 tcacgaatgg ggatttttga ctatgaaacg gaatgcgaaa actatcatcg cagggatgat
      241 tgcactggca atttcacaca ccgctttagc cgacgatatt aaagttgccg ttgtcggcgc
      301 gatgtccggc ccgattgccc agtggggcga tatggaattt aacggcgcgc gtcaggcgat
      361 taaagacatt aatgccaaag ggggaattaa aggcgataag ctggttggcg tagaatatga
      421 cgacgcctgc gacccgaaac aagccgttgc ggtcgccaac aaaatcgtta atgacggcat
      481 taaatacgtt attggtcatc tgtgttcttc ttctacccag cctgcgtcag atatctatga
      541 agacgaaggt attcttatga tctcgccggg agcgaccaac ccggagctga cccaacgcgg
      601 ttatcaacac attatgcgta ctgccgggct ggactcttcc caggggccaa cggcggcaaa
      661 atacattctt gagacggtga agccccagcg catcgccatc attcacgaca aacaacagta
      721 tggcgaaggg ctggcgcgtt cggtgcagga cgggctgaaa gcggctaacg ccaacgtcgt
      781 cttcttcgac ggtattaccg ccggggagaa agatttctcc gcgctgatcg cccgcctgaa
      841 aaaagaaaac atcgacttcg tttactacgg cggttactac ccggaaatgg ggcagatgct
      901 gcgccaggcc cgttccgttg gcctgaaaac ccagtttatg gggccggaag gtgtgggtaa
      961 tgcgtcgttg tcgaacattg ccggtgatgc cgccgaaggc atgttggtca ctatgccaaa
     1021 acgctatgac caggatccgg caaaccaggg catcgttgat gcgctgaaag cagacaagaa
     1081 agatccgtcc gggccttatg tctggatcac ctacgcggcg gtgcaatctc tggcaactgc
     1141 ccttgaacgt accggcagcg atgagccgct ggcgctggtg aaagatttaa aagctaacgg
     1201 tgcaaacacc gtgattgggc cgctgaactg ggatgaaaaa ggcgatctta agggatttga
     1261 ttttggtgtc ttccagtggc acgccgacgg ttcatccacg gcagccaagt gaaaatccca
     1321 ccgcccgtga aatgcgggcg ggtttagaaa ggttacctta tgtctgagca gtttttgtat
     1381 ttcttgcagc agatgtttaa cggcgtcacg ctgggcagta cttacgcgct gattgccatc
     1441 ggctacacca tggtttacgg cattatcggc atgatcaact tcgcccacgg cgaggtttac
     1501 atgatcggca gctacgtctc atttatgatc atcgccgcac tgatgatgat gggcattgat
     1561 accggctggc tgctggtagc ggcgggattc gtcggcgcaa tcgtcattgc cagcgcctac
     1621 ggctggagta tcgaacgggt ggcttaccgc ccggtgcgta actctaagcg cctgattgca
     1681 ctcatctcgg caatcggtat gtccatcttc ctgcaaaact acgtcagcct gactgaaggt
     1741 tcgcgcgacg tggcgctgcc cagcttgttt aatggtcagt gggtagtggg tcatagcgaa
     1801 aacttctctg cctctattac caccatgcag gcggtgatct ggattgtcac cttcctcgcc
     1861 atgctggcgc tgacgatttt cattcgttat tcccgtatgg ggcgcgcctg ccgtgcctgc
     1921 gccgaagatc tgaaaatggc gagcctgctt ggcattaaca ccgaccgggt gattgctttg
     1981 acctttgtga ttggcgcggc gatggcggcg gtggcgggcg tactgctcgg tcagttctac
     2041 ggcgtcatta acccctatat cggctttatg gccgggatga aagcctttac cgctgcggtg
     2101 ctcggtggga ttggcagcat tccgggagcg atgattggcg ggctgattct ggggattgcg
     2161 gaggcgctct cttctgccta cctgagtacg gaatataaag atgtggtctc attcgccctg
     2221 ctgattctgg tgctgctggt gatgccgacc ggtattctgg gtcgcccgga ggtagagaaa
     2281 gtatgaaacc gatgcatatt gcaatggcgt tgctctctgc cgcgatgttt tttgtcctgg
     2341 cgggcgtctt tatgggcgta caactggagc tggatggcac caaactggtg gtcgacacgg
     2401 cttcagacat ccgttggcag tgggtgttta tcggcacggc ggtggtcttt ttcttccagc
     2461 ttttgcgacc ggctttccag aaagggttga aaagcgtttc cggaccgaag tttattctgc
     2521 ccgccattga tggctccacg gtgaagcaaa agctgtttct cgtggcgctg ctggtgcttg
     2581 cggtggcgtg gccgtttatg gtctcacgcg ggacggtgga tatcgccacc ctgaccatga
     2641 tctacattat cctcggactc gggctgaacg tggtggtagg gctttctggt ctgctggtgc
     2701 tggggtacgg cggcttttac gccatcggcg cttatacctt tgcgctgctc aatcactatt
     2761 acggcttggg cttctggacc tgtctgccga tagccgggtt gatggcggcg gcggcgggct
     2821 tcctgctcgg cttcccggtg cttcgcttgc gcggtgacta tctggcgatc gttaccctcg
     2881 gtttcggcga aattgtgcgc atattgctac tcaataacac cgaaattacc ggcggtccga
     2941 acggtatcag tcagatcccg aaaccgactt ttttcggcct tgagttcagc cgtaccgctc
     3001 gtgaaggcgg ctgggacacg ttcagtaatt tctttggcct gaaatacgat ccctccgatc
     3061 gtgtcatttt cctctacctg gtggcgttgc tgctggtggt gctaagcctg tttgtcatta
     3121 accgcctgct gcggatgccg ctgggtcgcg cctgggaagc gttgcgtgaa gatgaaatcg
     3181 cctgccgttc gctgggctta agcccgcgtc gtatcaagct gactgccttt accatcagcg
     3241 ccgcgtttgc cggttttgcc ggaacgctgt ttgctgcgcg tcagggcttt gtcagcccgg
     3301 aatccttcac ctttgccgaa tcggcgtttg tgctggcgat agtggtgctc ggcggtatgg
     3361 gctcgcaatt tgcggtaatt ctggcggcaa ttttgctggt ggtgtcgcgc gagttgatgc
     3421 gtgatttcaa cgaatacagc atgttaatgc tcggtggttt gatggtgctg atgatgatct
     3481 ggcgtccgca gggcttgctg cccatgacgc gcccgcaact gaagctgaaa aacggcgcag
     3541 cgaaaggaga gcaggcatga gtcagccatt attatctgtt aacggcctga tgatgcgctt
     3601 cggcggcctg ctggctgtga ataacgtcaa tcttgaattg tacccgcagg agatcgtctc
     3661 gttaatcggt cctaacggtg ccggaaaaac cacggtcttt aactgcctga ccggattcta
     3721 caaacccacc ggcggcacta ttttactgcg cgatcagcat ctggagggtt tgccggggca
     3781 gcaaatcgcc cgcatgggcg tggtgcgtac cttccagcat gtgcgtctgt tccgcgaaat
     3841 gacggtaatt gaaaacctgc tggtggcgca gcatcagcaa ctgaaaaccg ggctgttctc
     3901 tggcctgttg aaaacgcctt ctttccgtcg cgcccaaagt gaagcgctcg accgcgccgc
     3961 cacctggctt gagcgcattg gtttgctgga acacgccaac cgtcaggcga gtaacctggc
     4021 ctacggtgac cagcgccgtc tggagattgc ccgctgcatg gtgacgcagc cggagatttt
     4081 aatgctcgac gaacctgcgg caggtcttaa cccgaaagaa acgaaagagc tggatgagct
     4141 gattgccgaa ctgcgcaatc atcacaacac cactatcttg ttgattgaac acgatatgaa
     4201 gctggtgatg ggaatttcgg accgaattta cgtggtcaat caggggacgc cgctggcaaa
     4261 cggtacgccg gagcaaatcc gtaataaccc ggacgtgatc cgtgcctatt taggtgaggc
     4321 ataagatgga aaaagtcatg ttgtcctttg acaaagtcag cgcccactac ggcaaaatcc
     4381 aggcgctgca tgaggtgagc ctgcatatca atcagggcga gattgtcacg ctgattggcg
     4441 cgaacggggc ggggaaaacc accttgctcg gcacgttatg cggcgatccg cgcgccacca
     4501 gcgggcgaat tgtgtttgat gataaagaca ttaccgactg gcagacggcg aaaatcatgc
     4561 gcgaagcggt agcgattgtc ccggaagggc gtcgcgtctt ctcgcggatg acggtggaag
     4621 agaacctggc gatgggcggc ttttttgccg aacgtgatca gtttcaggag cgcataaagt
     4681 gggtgtatga gctgtttcca cgtcttcatg agcgtcgtgt tcagcgggca ggcaccatgt
     4741 ccggcggtga acagcagatg ctggcgattg gtcgcgcgct gatgagcaat ccgcgtttgt
     4801 tgttgcttga tgagccatcg ctcggtcttg cgccgattat catccagcaa attttcgaca
     4861 ccatcgagca gctgcgcgag caggggatga ctatcttcct cgtcgagcag aacgccaacc
     4921 aggcgctgaa gctcgctgat cgcggctacg tgctggaaaa cggccatgtt gtgctctccg
     4981 atactggtga tgcgctgctg gcgaacgaag cagtgagaag tgcgtattta ggcgggtaaa
     5041 ttatcgataa cccggcagct aagttctgcc gggttattcg tccgtataca gacttgtttc
     5101 aactgaccgg gaggattaaa accagaccac ttatttgcca cggaaaggtt ccctcccatg
     5161 cattatgccg ttgttgtcat gcttgaactg ctttgctgta ttcacgctta ccgtaccgga
     5221 caagagcggt actggatttt tatcattttc tgttttccgg tgattggctg cgtggcgtat
     5281 ttcgtcatag tgatgctgcc tgaaacgggc gctgaccgtc acgggcatac tttgctgatg
     5341 cgcctgcagg acaaactcaa ccctgagcgc cacctgcgca aattaaccga agaactggcg
     5401 attgccgaga cgaatcaaaa ccattatgcg ttggctaatg aactggcgcg tctggggcgt
     5461 tatcacgaag ctgttcccca ttatcagcag gcgttaagcg ggatattcgc ccatgaagcg
     5521 gcaatgatgc taagcctggc gcaggcacag tttgctattc aggagttcgc cgcctgtcag
     5581 cagacgctgg aagatgtgat gcgttataac cctgattttc agtcggcaga cgggcatttg
     5641 ttatttgcca gaacgttagc cgcacaagaa aaatacgctg atgcggaaag cgagtttgaa
     5701 gtattgattt cttattaccc cggcccgcag gcgcgtattt actatgcgga aatgttggcg
     5761 aaaatgagtc ggttacgtga agccaacgag cagtatgttg ccgtggttga tacggctaaa
     5821 cgctcaagac ctcattatag aaaacaccac cgggaatgga ttaagaccgc caatgagcgg
     5881 ctgaagcaga gtgtggtgca atgaaaatct tctttgccgt tagaccagtg tcgcggcaat
     5941 cttttgttaa aattgaaaaa tgtagctctc atcgggcagc aaaattggac gcagaatttg
     6001 ctgccatttt tgctcttcat ggtaaggagt tctggcgatt agctggccgt taatcgcctc
     6061 ttttcagtgc attctcggga tatccgtgcc ccatctccca ttttccctca cccgtaccgt
     6121 caccgccttg tcatctttct gacaccttac tatcttacaa atgtaacaaa aaagttattt
     6181 ttctgtaatt cgagcatgtc atgttacccc gcgagcataa aacgcgtgaa ttcgcgcatt
     6241 cggtacaaca agagagataa acgatgaaac cgttacatta tacagcttca gcactggcgc
     6301 tcggactggc gttaatgggg aatgcacagg cagtgacgac cattccgttc tggcattcta
     6361 tggaagggga actgggtaaa gaggtggatt ctctggccca acgttttaac gccgaaaacc
     6421 cggattacaa aattgtaccg acctataaag gcaattacga acagaattta agcgcgggga
     6481 ttgccgcatt tcgtaccggc aatgctccgg ctattttgca ggtttatgaa gttggcaccg
     6541 ccaccatgat ggcgtcgaaa gccattaaac cggtttatga cgtgtttaaa gaggcgggga
     6601 ttcaattcga tgagtcgcag tttgtgccga cggtttccgg ttactactcc gacagcaaaa
     6661 cgggccactt actctcccag ccattcaaca gctcgactcc cgttctctat tacaacaaag
     6721 acgccttcaa gaaagcgggg ttagacccgg aacaaccgcc gaaaacctgg caggatctgg
     6781 cggactatgc cgcaaaacta aaagcctccg gcatgaagtg cggctacgcc agcggctggc
     6841 agggctggat ccaactggaa aactttagcg cctggaacgg tctgccgttt gccagcaaaa
     6901 acaacggctt tgacggcacg gatgcggtgc tggagttcaa caagccggag caggtgaaac
     6961 acatcgccat gctcgaagag atgaacaaga agggcgattt cagctacgtc gggcgtaagg
     7021 atgaatccac cgagaagttc tataacggtg attgcgctat gaccaccgcc tcttccggtt
     7081 ctctcgctaa tattcgcgag tacgccaaat tcaactatgg cgtaggcatg atgccttacg
     7141 acgccgatgc gaaagatgca ccgcaaaacg ccattatcgg cggggccagc ctgtgggtga
     7201 tgcagggcaa agataaagaa acgtataccg gcgtggcgaa gttcctcgac ttcctcgcga
     7261 agccagaaaa cgctgccgag tggcatcaga aaaccggtta tctgccaatc accaaagcag
     7321 cgtatgacct gacccgtgag cagggctttt atgagaaaaa cccaggggcg gataccgcga
     7381 cgcgtcagat gctgaataag ccgccgttgc cgttcaccaa agggctgcgt ctgggcaaca
     7441 tgccgcagat ccgcgtgatt gtggatgaag agctggagag cgtgtggacc ggtaagaaga
     7501 caccacagca ggcactggat accgccgttg agcgtggaaa tcagttgctg cgccgctttg
     7561 agaaatcgac gaagtcttaa tcagtgtaat gtcggatgcg tttcgcttat ctgacctggc
     7621 atcgcgtgta ggccggataa gcgaagcgca tccggcacag ttcaggaatt aaccgtaatg
     7681 tcatcatccc gtccggtgtt ccgctcgcgc tggctgcctt atctgctggt cgcgccgcag
     7741 ctcatcatca ccgttatctt ttttatctgg cctgcgggcg aagcgttgtg gtactcgctg
     7801 caaagcgtcg atccgtttgg tttctccagc cggtttgtcg gcctggataa cttcgtcgcg
     7861 ctgtttcatg acagctacta tatcgactcc ttctggacga cgataaaatt cagcaccttt
     7921 gtcaccgtca gcggtttgct ggtatcgctg ttctttgcgg cgctggtgga gtacatcgtg
     7981 cgcggcagtc gtttctatca aaccttgatg ctgctgccgt atgccgtggc tcccgccgtt
     8041 gccgccgtat tgtggatctt cctgtttaac cccggtcgcg ggctgatcac ccattttctc
     8101 gctgagttcg gctacgactg gaaccacgcg caaaacagcg gtcaggcaat gtttctggtg
     8161 gtgtttgcct cagtatggaa gcaaatcagc tacaacttcc tgttcttcta tgccgcgctg
     8221 caatccattc cccgttcgtt gatcgaagcc gcagccatcg acggtgcagg gccgattcgc
     8281 cgcttcttta agattgcgct gccgcttatc gccccggtga gtttcttcct gctggtagtg
     8341 aacctggtgt atgccttctt cgacaccttc ccggtgatcg acgccgccac gtccggcggg
     8401 ccggttcagg ccaccactac gctgatttat aagatctacc gcgaaggctt taccggactg
     8461 gatctggctt cgtctgccgc acagtcggtg gtgttgatgt tcctcgtcat cgtgctgacg
     8521 gtgatgcagt tccgctatgt tgaaagcaag gtgcgttacc aatgattgag aaccgtccgt
     8581 ggctgacgat attcagccat accatgctga tcctcgggat cgcggtgatc ctattcccgc
     8641 tgtacgtggc gtttgtcgcg gcgacgctgg ataaacaggc cgtctacgcc gcgccgatga
     8701 cgctcatccc cggcacgcat ctgctggaaa acatccacaa catctgggta aacggggtag
     8761 gcacaaatag tgcgccgttc tggcggatgt tgcttaacag cttcgtgatg gcgttcagca
     8821 ttacgctcgg caaaattacc gtctcgatgc tctcggcatt cgccattgtc tggtttcgtt
     8881 ttccgctgcg taacctcttt ttctggatga tttttatcac cctgatgttg ccggttgaag
     8941 tacgtatctt cccgacggtg gaagtcatcg ccaacctgaa gatgctcgac agctacgcag
     9001 gtttaacgct gccgctgatg gcctcggcga ccgcgacgtt tcttttccgc cagttcttta
     9061 tgaccctacc ggatgaactg gtggaagccg cgcggatcga cggcgcgtcg ccaatgcgct
     9121 tcttttgcga catcgttttt ccgttgtcca aaactaatct ggcggcgttg tttgtgatca
     9181 cctttattta cggctggaac cagtatttgt ggccgttgtt gattattacc gacgtggatc
     9241 tcggcaccac cgtggcaggg atcaaaggga tgatcgccac tggcgaaggc accacagaat
     9301 ggaactcagt gatggcggcg atgttgttaa ccctgatccc accagtagtg attgttttag
     9361 tgatgcagcg tgccttcgtg cgcggcctgg tcgatagtga gaaataagat ggcaggactg
     9421 aaattacagg cagtaaccaa aagctgggat ggcaaaaccc aggtgattaa accgctgacc
     9481 cttgatgtgg cggatggcga atttatcgtg atggtcgggc cgtcaggctg cgggaaatcg
     9541 acgctgctgc gcatggttgc cgggctggag cgggtgacgg aaggcgatat ctgtatcaac
     9601 gaccagcggg tgaccgaaat ggagccgaaa gatcgcggga ttgcgatggt gttccagaac
     9661 tacgcgcttt atccgcatat gagtgttgaa gaaaacatgg cgtgggggct gaaaattcgc
     9721 ggcatgggca agcagcaaat tgccgagcgc gttaaagagg cggcgcgcat tctggaactg
     9781 gacggtctgc ttaagcgccg cccgcgcgag ctttccggcg gtcagcgtca gcgtgtggcg
     9841 atgggccggg cgattgtgcg cgatccggcg gtgttcctgt ttgatgagcc actctctaac
     9901 ctcgatgcca agctgcgcgt acagatgcgt cttgaactgc aacagctgca ccgtcgcctg
     9961 aaaacgactt cactctacgt tactcacgat caggttgagg cgatgaccct cgcccagcga
    10021 gtaatggtga tgaacggcgg cgttgccgaa cagattggca caccagttga agtctacgaa
    10081 aagcccgcca gcctgtttgt ggcgagtttt attggcagcc cggcgatgaa tctgctgaca
    10141 ggccgcgtga ataacgaagg cacgcacttc gagctggacg gcggcattgc gctgccgcta
    10201 aacggtggct accgtcagta tgcagggcgt aaaatgactc tcggcattcg cccggaacat
    10261 atcgcgctaa gctcgcaggc agaaggcggc gtgccgctgg tgatggacac gctggagatc
    10321 ctcggcgcag ataacctggc gcacggacgc tggggcgaac agaagctggt ggtacggctg
    10381 gcgcatcagg agcgcccgac ggcaggcagc acgctgtggc tgcatctgcc ggaaaatcag
    10441 ctacatcttt ttgatggtga aacaggacaa cgagtatgag taactggcct tatccccgca
    10501 tcgtcgccca ccgtggcggc ggtaaactgg ccccggaaaa caccctggcg gcaatcgacg
    10561 tcggggcaaa atacggtcat aagatgatcg aatttgacgc gaagttgtcg aaagatggcg
    10621 aaatcttcct gctccatgac gacaatctcg aacgtaccag caacggctgg ggcgtcgcgg
    10681 gtgaactgaa ctggcaggat ttactacgtg tggatgcagg cagttggtac ggcaaagcgt
    10741 ttaaaggtga gccgctgccg ttgctttcgc aggtggcgga acgctgtcgc gaacatggga
    10801 tgatggcgaa tatcgaaatc aaacccacca ctggcactgg gccattaacg ggcaaaatgg
    10861 tggcgctggc ggcacgcgag ctgtgggccg gtatgacgcc gccgctactg tcatcgtttg
    10921 agattgatgc tttagaagcc gcacaacagg cagtaccaga actgccgcgc ggtttgttgc
    10981 tggatgagtg gcgcgacgac tggcgagaac tgaccgcgcg gctgggctgc gtctctattc
    11041 atctcaatca taagttgctc gataaagcgc gagtgatgca gttgaaagac gccggattac
    11101 ggattctggt ttataccgtc aataaacccc agcgcgcggc agagttgctg cgctggggcg
    11161 tggattgcat ctgtaccgat gcgattgatg tgattggtcc gaactttacg gcccaatagt
    11221 tttcaacggt atatcaggct gcggcgtgcc gggctgattc agcatgtcgc cgtttctcct
    11281 ctccggcaac atatgctgct gattcaacga actatccgga ttacggttgc tgtttaacat
    11341 cccgccattg gtgttgggca gcatttgctg ccgggcggga tttcgctccc ccggctgtga
    11401 ctgcaacacc cgctgagaat tattgtttat ctggttttct aaatgctgtt gttgcaactg
    11461 agtttgcgtt ttcagttgct gattcagcat ccctttttgc tggatttgct gcgtttgcat
    11521 ctgggtttgc atccgctgct gactggggat ctgatacccc ggctggttag ggttgttcag
    11581 agtattaatg ggctgtgcaa agccgacaaa cggcaggagt gccgtaagaa tcagaagtcg
    11641 tttcatcgcg tatcctcctc tgaagatatt ctttaagttt actcctttcc tgcaatcatc
    11701 aggattaatt cagagttata taccaggctt agctggggtt gccccttaat ctctggagaa
    11761 taacgatgat aaaaccgacg tttttacgcc gggtggccat tgctgctctg ttcacaggaa
    11821 gttgttttag tacggtagcc gcgccacctg cgccgtctcc tgtttcttac ggcgtggagg
    11881 aagatgtctt tcatccggta cgcgcgaaac aggggatggt ggcatctgta gatgctactg
    11941 ccactcaggt gggggtggat atcctcaagg agggcgggaa tgccgttgat gccgccgtgg
    12001 ctgtgggcta cgcgctggcg gtaacgcatc cgcaggcagg gaatctgggc ggtggtggtt
    12061 ttatgctgat ccgctcgaaa aatggcaata ccaccgctat cgatttccgc gaaatggcac
    12121 ccgccaaagc gacccgcgat atgttccttg atgaccaggg caacccggac agcaaaaaat
    12181 cactcacttc gcatctggct tccggcacgc ccggtacggt cgctggtttc tcgctggcgt
    12241 tggataaata cggcacaatg ccgttgaaca aagtggtgca gccagcgttt aaactggcac
    12301 gcgatggttt tatcgttaac gacgagctgg ctgacgatct caaaacctac gggagcgaag
    12361 tgctgccgaa tcacgaaaac agcaaggcta tcttctggaa agagggcgag ccgctgaaaa
    12421 agggcgacaa gctggtgcag gcgaacctgg caaagagcct ggagatgatt gccgaaaacg
    12481 gcccggacga attctacaaa ggcacgatag cggaacagat tgcccaggag atgcagaaaa
    12541 acggtggcct gatcactaaa gaagatttag cggcgtataa agcggtcgaa cgcacaccga
    12601 taagcggcga ttatcgcggg tatcaggttt actccatgcc accgccatcc tccggcggga
    12661 tccatatcgt acaaatcctc aatattctgg aaaacttcga tatgaagaaa tacggttttg
    12721 gcagcgccga cgcgatgcag atcatggcag aagcggagaa atatgcctac gctgaccgct
    12781 cggaatatct tggcgacccg gattttgtga aagtcccgtg gcaggcgctg accaataaag
    12841 cctatgccaa atctattgcc gagcaaatcg atatcaataa agcgaaaccg tccagcgaga
    12901 ttcgccccgg caagcttgcg ccttatgaga gtaatcaaac tacccattat tcggtggtgg
    12961 ataaagacgg taacgcggtg gcggtgacct atacgctgaa caccaccttc ggtacgggta
    13021 ttgtcgcggg cgagagcggt attttgctca ataaccaaat ggatgatttc tctgccaaac
    13081 cgggcgtacc gaacgtttac gggctggtgg gcggtgatgc caacgccgtc gggccgaaca
    13141 aacgcccgct gtcatcgatg tcaccaacca ttgtggtgaa agacggtaaa acctggctgg
    13201 tcaccggtag ccctggcggg agcctgatca tcactacagt gcttcaaatg gtggtgaata
    13261 gcatcgatta tggcatgaac gtcgccgaag cgaccaatgc gccgcgtttc caccatcagt
    13321 ggttgccgga cgagctgcgt gtcgaaaaag ggtttagccc ggatacgctc aagctgctgg
    13381 aagcaaaagg tcagaaagtg gcgctgaaag aggcgatggg cagtacacaa agcattatgg
    13441 ttgggccgga cggtgagttg tacggcgcat ccgacccgcg ctcggtggat gatttaacgg
    13501 cggggtacta aggttagcgg ccctcttcgt gggaagaggg ctgttttctt gaacaagata
    13561 aattagtgtc tggattgctt ttactattgc tccgtaatat ataatcataa ttatcctgaa
    13621 tgcttttaag gggcttaggt tatgctttct tttcaagagt atgcagaata gtctttcatt
    13681 taccgtgaaa ataggacgtg cgataattac ttcctgatat tcaggaaagg aattaactat
    13741 gctatt
//
LOCUS       DAZKFZ010000112        13634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000112 DAZKFZ010000000
VERSION     DAZKFZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(54..878)
                     /gene="dinD"
                     /locus_tag="ACHOQW_002993"
     CDS             complement(54..878)
                     /gene="dinD"
                     /locus_tag="ACHOQW_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP9574038.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(1099..1962)
                     /locus_tag="ACHOQW_002994"
     CDS             complement(1099..1962)
                     /locus_tag="ACHOQW_002994"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9574039.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            2089..2805
                     /gene="rph"
                     /locus_tag="ACHOQW_002995"
     CDS             2089..2805
                     /gene="rph"
                     /locus_tag="ACHOQW_002995"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9574040.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            2871..3512
                     /gene="pyrE"
                     /locus_tag="ACHOQW_002996"
     CDS             2871..3512
                     /gene="pyrE"
                     /locus_tag="ACHOQW_002996"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9574041.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3549..4145)
                     /gene="slmA"
                     /locus_tag="ACHOQW_002997"
     CDS             complement(3549..4145)
                     /gene="slmA"
                     /locus_tag="ACHOQW_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP9574042.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(4252..4710)
                     /gene="dut"
                     /locus_tag="ACHOQW_002998"
     CDS             complement(4252..4710)
                     /gene="dut"
                     /locus_tag="ACHOQW_002998"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9574043.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(4688..5908)
                     /gene="coaBC"
                     /locus_tag="ACHOQW_002999"
     CDS             complement(4688..5908)
                     /gene="coaBC"
                     /locus_tag="ACHOQW_002999"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP9574044.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            6080..6748
                     /gene="radC"
                     /locus_tag="ACHOQW_003000"
     CDS             6080..6748
                     /gene="radC"
                     /locus_tag="ACHOQW_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9574045.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            6965..7201
                     /gene="rpmB"
                     /locus_tag="ACHOQW_003001"
     CDS             6965..7201
                     /gene="rpmB"
                     /locus_tag="ACHOQW_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9574046.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            7222..7389
                     /gene="rpmG"
                     /locus_tag="ACHOQW_003002"
     CDS             7222..7389
                     /gene="rpmG"
                     /locus_tag="ACHOQW_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9574047.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            7487..8296
                     /gene="mutM"
                     /locus_tag="ACHOQW_003003"
     CDS             7487..8296
                     /gene="mutM"
                     /locus_tag="ACHOQW_003003"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP9574048.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(8335..8814)
                     /gene="coaD"
                     /locus_tag="ACHOQW_003004"
     CDS             complement(8335..8814)
                     /gene="coaD"
                     /locus_tag="ACHOQW_003004"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9574049.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(8822..10099)
                     /gene="waaA"
                     /locus_tag="ACHOQW_003005"
     CDS             complement(8822..10099)
                     /gene="waaA"
                     /locus_tag="ACHOQW_003005"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP9574050.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            10512..11570
                     /gene="rfaQ"
                     /locus_tag="ACHOQW_003006"
     CDS             10512..11570
                     /gene="rfaQ"
                     /locus_tag="ACHOQW_003006"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP9574051.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            11567..12691
                     /locus_tag="ACHOQW_003007"
     CDS             11567..12691
                     /locus_tag="ACHOQW_003007"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9574052.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            12684..13481
                     /gene="rfaP"
                     /locus_tag="ACHOQW_003008"
     CDS             12684..13481
                     /gene="rfaP"
                     /locus_tag="ACHOQW_003008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP9574053.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         3357 a         3306 c         3544 g         3427 t
ORIGIN      
        1 ctttggctta cgcatcgggt atctcaatca acaaaaagag tttttagttt ctgctatttc
       61 ttctctgtaa tttttacact gttttccaac tgcttgatgc tgacctgcgg ggtcggcaac
      121 tcctcaggca tggttccgcc aagttcctga atggtttgcc tcaccttgct acccacatca
      181 aagtgtgtgg tattagcttg ctgttttgaa ttcacgccat cccgcttgag tttttcttct
      241 gtttgggtag ctcgaaagag attagccgcc agttctgttg aacccatatg atccaggatc
      301 ttctgactct ttttcagccc cttcagttgg tggatggctt tctgatctaa tccaccatac
      361 agcccctggt aaccatgatt ctgaaatatg gcgaaatcag tagctgtcgc taccgcagct
      421 tgctgtgctg cctcaaccaa ttgtttgtta tgttctttca actcatttct cagaaacaga
      481 cgcttttcgt cttcacgaag ttgcttaaat gcttcatcgt cagcaagctc ctgccgtcgg
      541 gtctggatag caaaataagt ctgccctgcc gcaatgaccg gtttcgcagg gtcgccgttt
      601 tgtactacca ggtaacaggc atagcgggag agatgaacat cttcaagttc tcgttgagcc
      661 cctgaaccta atacaaccat tttggtggtt tcaacgaaat ggtcagatgc agcttgattg
      721 ctggcttcgc acgcttgagt cgcgcgggcc agtacctttt gaaaattgcg ccagtcccga
      781 taatccagta acggtgccaa ttctctagct gaccaaaatt cttgcccttc agtgccataa
      841 tgccgtattt cttcaaaagg ttgatgatgt tcgttcattg cccactccat gtgatatcga
      901 ttgcaaagaa agtaaatctg taactgtatt tatatacagt tgtgagtatc tcacgttctc
      961 agtgtgctgg gcaaaaaaac atcacaaaat ctggagcagg cacgcaaaac agaattgaag
     1021 ttgcggggaa gaaaaatgcc gctctgtttg agtgggcggc atttgtgaag gctgaaaagt
     1081 aagaattggc gttacgagtt attcaatgtt ctgaatctgc tcgcgcatct gctcaatcaa
     1141 cactttcagc tcaatcgcgg agtttgtcac ttcggcattg atagacttcg acgcaagagt
     1201 gttcgactcg cggttgaatt cctgcatcat aaagtccaga cgacgaccaa cggcttcttt
     1261 tttcttcaga atgttgtagg tctctttgac atgcgcttcc aggcggtcca gttcttcggc
     1321 aacgtcaatt cgttgtgcca gcagaaccag ctcctgctcc agacggttgt tttccaactg
     1381 aacctgagca tcttccagct tcgcgaccag acgctcacgc tgccattgca ggatttccgg
     1441 catatgggcg cggactttga ccacttcggc ggtgacgcct tccagacgct gctcgatcaa
     1501 tgctttcagt gcctgacctt cggtttcgcg cgcgacaata aagtcgtcca gcgtaccatc
     1561 cagcgccgcg agaatttcag cggcaatggc gtcaagatcc tgctcctggg ctgccatcac
     1621 gcccggccag cgtagaatat caaccgggtt gatttcccct tcgtcactct gcatttttac
     1681 ccagttcgcg gcagttacca gctgtttagc cagtttttcg ttgaggatca gctcaccttg
     1741 cgcgctaaca tctggctcat agcgcagggt acattccact ttaccgcgcg tcaggcgaga
     1801 acgaatacgc tcgcgaacga caggttcaag gctacggaac tgctccggca gacgaaagta
     1861 agtttccaga taacgctggt ttaccgagcg catttcccag gttgcgctcc cccattcacc
     1921 cttgatttca cgccgggcgt aggcggtcat actgcggatc atagacgttc ctgtttataa
     1981 aaggagaggt ggaaggatta tagccatcga tgccttgtca ggataggaat aaccgccgga
     2041 agtccgtata atgcgcagcc acatttgttt caagccggag atttcaatat gcgtccagca
     2101 ggccgtagca ataatcaggt gcgtcccgtt accctgactc gtaactatac aaaacatgca
     2161 gaaggctcgg tgctggtcga atttggcgat accaaagtgt tgtgtaccgc ctctattgaa
     2221 gaaggcgtgc cgcgcttcct gaaaggtcag ggccagggct ggatcaccgc agagtacggc
     2281 atgctgccac gttctaccca cacccgtaac gctcgtgaag cggcgaaagg taagcagggt
     2341 ggacgcacaa tggaaatcca gcgtctgatc gcccgtgctc ttcgcgcggc agtagatttg
     2401 aaagcgctgg gtgagttcac cattacgctg gactgcgacg tgcttcaggc tgatggtggc
     2461 acgcgtaccg cgtcgattac gggtgcctgc gtggcgctgg cagatgcgct acagaagctg
     2521 gtggaaaacg gcaagctgaa aaccaacccg atgaaaggga tggtagccgc agtttctgtc
     2581 ggaattgtga acggcgaagc ggtttgcgat ctggaatacg ttgaagactc tgccgcagag
     2641 accgacatga acgtagtgat gaccgaagac gggcgcatca ttgaagtgca ggggacggca
     2701 gaaggcgagc cgttcaccca tgaagagcta ctcaccttgt tggctctggc ccgaggggga
     2761 atcgaatcca ttgtagcgac gcagaaggcg gcgctggcaa actgattttt aaggcgactg
     2821 atgagtcgcc ttttttttgt ctgtagaaaa gtaagatgag gagcgaaggc atgaaaccat
     2881 atcagcgcca gtttattgaa tttgcgcttg gcaagcaggt gttaaagttt ggcgagttta
     2941 cgttgaagtc cgggcgcaaa agcccctatt tcttcaacgc cgggctgttt aataccgggc
     3001 gcgatctggc actgttaggc cgtttttacg ctgaagcgtt ggtggattcc ggccttgagt
     3061 tcgatctgct gtttggccct gcttacaaag ggatcccgat tgccaccact accgccgtgg
     3121 cgctggcgga gcatcatgac cttgacctgc cgtactgctt taaccgcaaa gaggcaaaag
     3181 accacggtga aggcggcaat ctggttggta gcgcgttaca aggacgcgta atgctggtag
     3241 atgatgtgat caccgccgga acggcgattc gcgaatcgat ggagattatt caggctaatg
     3301 gcgcgacgct tgctggcgtg ttgatttcgc ttgatcgtca ggaacggggg cgcggcgaga
     3361 tttcggcgat tcaggaagtt gagcgtgatt acaactgcaa agtgatctct atcatcaccc
     3421 tgaaagacct gattgcttac ctggaagaga agccggaaat ggcggaacat ctggcggcgg
     3481 ttaaggccta tcgcgaagag tttggcgttt aaagaaactc gccggatgaa aagtcatccg
     3541 gcgtcatatt actgcaactg tgccgcaatt agcggccagc gggcgtcaaa atcatccgtc
     3601 gggcggtatt taaattcgct gcggacaaaa cgtgacagca taccttcaca gaaggctagg
     3661 atctggcttg ccagcagggt ttcatcggtg gtgtaacctt caccctcacg cattctcttt
     3721 tcacgcaata cctgacgcag ctgcgcttca atacgctcga acagctggtt gatgcgccct
     3781 tgcaggcgat cctgttcaaa cattagcgca tgaccagtga ggatgcgggt caggccagga
     3841 ttacgctcac caaaaccgag aagcagcaac acaatcagac gcaggcgcgc tgtggtgtct
     3901 ttctcatctt tcagaatcag attgatgcga gtaatcaggc tatcttcgat aaactcaatc
     3961 aggctatcga acatgcgggt cttactgggg aagtggcgat acagtgccgc ttcggaaacg
     4021 ccgacagagg cggccagttt tgccgtcgtg atacgttggc ttccatcgct ggattccagc
     4081 atcagcgcca gagactgaag tatttcctcg cgacggttcc ttttcgcagt ttgtttttct
     4141 gccatgttac aaaatacccc tgaaaataag cacttgccag gcggcaccca cgctatgacc
     4201 gcaaacgaaa tgtttacggc tatgttatga cgttattcgg atgcgtatgt gttactgacg
     4261 accagagtga ccaaagccgc cttcaccgcg gtcggtggcg tcgaaatctt ccaccagatt
     4321 aaattcagcc tgtactaccg gaacaaaaat catctgggcg atacgttcgc caggttgaat
     4381 ggtgaagctg tcctgaccac ggttccacac ggaaatcatc aactggccct gatagtcgga
     4441 atcgatcaat cctaccaggt taccaagcac gataccgtgc ttatgtccca atccggagcg
     4501 cggcagcatc attgccgcca gtgaaggatc ggcaatatga atcgccagcc cggtcggaac
     4561 cagcgtagtg tcacccggag ccagttctac ggcgtcgtcg agacaggcac gcaggtcaag
     4621 tccggcagag ccagaggtgg cataagtcgg gagcggaaat tccttcccaa cgcgcgggtc
     4681 cagaatctta acgtcgattt ttttcatcat aacgggtcac gatctcgtcg agtaataatt
     4741 ggccaaggag ctctttgcgc tcaagcggta agactttatc tccgtcctgc cagaaaaggt
     4801 gtaatgcgtt gttgtcgctg ttaaatcctt gagttggctg ggaaacatcg ttcgcgcaga
     4861 tcagatcaag gtttttacgg atacgttttt gccgggcgta ttcttccaca ttatttgttt
     4921 cggcggcaaa tccaacgacg tagggtcgat ggtcttttag tgcggcaacg cctgcgacga
     4981 tatcggggtt tttaaccatt tttattgtta attcatcacc ctgcgtggcc tgctttttga
     5041 ttttctctgg ggccacggta gctgcgcgat aatccgccac ggcggcgcag ccgataaaaa
     5101 tattttgctg ctgtacagaa gcattcacgg cggcttccat ttccagcgcg gtcatcacat
     5161 caacacgttt aacaaacggt ggcgtcggta gtgaaaccgg acctgatacc agcgtgacgt
     5221 tcgcgccacg acgggcagcg gcggcggcga tagcaaaacc catcttgccg gagctgtgat
     5281 tagagatata acgcacggga tcgagcggtt cacgcgtcgg gccggcggta atcataatgt
     5341 tcagatgttt caggtcgttg acgggcgaaa aatgcgctac cgccatatcc acaatggtta
     5401 acggatcgag cattcgccca ggaccgatat caccacaagc ctgactgcca ctgtctggcc
     5461 cccagatgag caaaccacgg gaagcaagca cctctaaatt atgctgcgtg gcagcggcac
     5521 ggtacatctg ctggttcatg gcggggagca cggctacagg cgcaggtgta gccagacaaa
     5581 tcgtcgatac caggtcattc gccattccgg cagcaacacg ggcaatcaaa tctgccgtgg
     5641 caggggcgag aatcactaaa tcagcccatt tacccagctc aatatggccc atagcggctt
     5701 cggctgccgg gtccagcaaa ctgtcggaaa cgggataacc agaaaccgcc tgcaagctaa
     5761 gtggggtgat aaaggctttt gccgcttcgg tcatggctac gcggacgtcg gccccgcgat
     5821 cgcgcaaacg acgcaccagt tcaggggttt tataggcagc aataccgccg ctaacgccga
     5881 gaacgatttt tttaccggcc aggctcatga tgatttttcc tgttgggtga caccagaagt
     5941 tggcgatttt atcacaatac ttttgttgtc gtgccttcac ctgagattca ctttgcgagg
     6001 cgctttccag gattgaaaac tggccgtcga tttaacggaa cggctatgac aggatgcgag
     6061 caccacaaag gaggtgaagg tgaaaaacaa tgcacagctg ttgatgccgc gcgaaaaaat
     6121 gctgaagttt ggtattagcg ccttaactga tgtcgagctg ctggcgctat ttctgcgtac
     6181 cggaacgcgc ggtaaagatg tattaaccct ggcaaaagag atgctggaga atttcggctc
     6241 tctttatggc ttgttaacct ctgaatatga acaatttagt ggtgttcatg gaattggcgt
     6301 agcgaaattt gcccagttaa aggggattgc tgaactggcg cgacgttact acaacgtgcg
     6361 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
     6421 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactccc aacaccgggt
     6481 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
     6541 aattatccgc gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
     6601 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
     6661 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
     6721 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
     6781 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
     6841 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
     6901 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
     6961 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
     7021 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
     7081 ggttgagagc gagaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
     7141 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     7201 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     7261 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     7321 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     7381 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     7441 ctgcttgccc ccatattgac tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     7501 cgaagttgaa accagccgcc gcggcataga accgcatctc gttggtgcaa ccattcttca
     7561 tgcagtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     7621 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     7681 gggctggatt atcattcatt tagggatgtc tggcagcctg cgcatccttc cagaagaact
     7741 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     7801 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcataatgt
     7861 gctggcccat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     7921 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     7981 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     8041 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     8101 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     8161 taaaccgggc tatttcgctc aggaattgca ggtttacggg cgaaaaggtg agccgtgtcg
     8221 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     8281 gcagtgccag aagtaattca tgcgcgccgg atggcatacc atccggcata aacgctacgc
     8341 taacttcgcc atcagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     8401 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     8461 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     8521 atattcaaaa tctgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     8581 ggcgaagttc gccatcaaat cactaaaccc gaccacttcc acgttcccca gatgcgcggt
     8641 tgcctgctgt gccagtgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     8701 ggcggcaatc gccagaataa cgtgatcgaa catctgcgtg gcgcgcgtca cgatatcgat
     8761 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     8821 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     8881 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     8941 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     9001 cgcttgcctg ctccagccgc gcgcaaatgt ctttaaagtt aaaagtatgc ggccccatca
     9061 ataccggaat tgcgtgtgcg gcagcttcca gcggattatg cccaccacgt tcaaccagtg
     9121 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     9181 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     9241 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     9301 ccaggatgag caataaattc gggaattgct gtaataaagc ctgatgtgcg gcaatgacca
     9361 cgctctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     9421 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     9481 ggctaccggt aacggttacc tggttatttt tcgcgcccag cgccacaaaa cgtgcaccat
     9541 cttcttcatt ttgggcggca atcagcgtaa tacgacgcag caagcgacgg acgaatttac
     9601 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     9661 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     9721 acaccagttt agggtcgact ttattcagga aacggttgag tgcatcgggc agatcatacg
     9781 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     9841 gcgtcatggt tgttacggta ataggtaaat caggataacg atgacgcagc gcgcgcacca
     9901 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     9961 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
    10021 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
    10081 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
    10141 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
    10201 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
    10261 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
    10321 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa atcattcggg tcggattgcc
    10381 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
    10441 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
    10501 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
    10561 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
    10621 aaatcgatgt gctgctttat caggacacca tcccgatcct gtctgaaaat ccagagatta
    10681 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
    10741 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
    10801 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
    10861 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttggtg ccgttgcagg
    10921 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
    10981 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
    11041 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
    11101 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
    11161 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
    11221 aaggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
    11281 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
    11341 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
    11401 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
    11461 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
    11521 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
    11581 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
    11641 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
    11701 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
    11761 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
    11821 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
    11881 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
    11941 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
    12001 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
    12061 accaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
    12121 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
    12181 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
    12241 gcgccacaac acattgctat atgttgttgg acaggataaa ccgcgaaaat ttgaggcgct
    12301 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
    12361 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
    12421 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
    12481 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
    12541 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgtc aggcctgggc
    12601 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
    12661 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
    12721 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
    12781 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
    12841 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
    12901 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
    12961 atggggtggc atttggcgaa aaaggcatga atccgctgac cagaacttca tttattatta
    13021 ccgaagatct gacaccaacc ataagtcttg aagattactg tgctgactgg gcgactaacc
    13081 ctccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcacgata
    13141 tgcatgctgc gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
    13201 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
    13261 ttcgcacgcg cgttccacgt cgttggcggg ataaagatct tattgggctt tatttttctt
    13321 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgccg
    13381 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
    13441 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta acccgtaact tatttttgcc
    13501 aaaattttgg atacagaata aatatgtctc aactcaatga tagtgacatc atcctttttg
    13561 agtataattt tcattatcaa aatataagat ctaaaaatac tcttgatata gcatttggta
    13621 ttgacagaaa tttt
//
LOCUS       DAZKFZ010000113        13484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000113 DAZKFZ010000000
VERSION     DAZKFZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            255..1352
                     /gene="mltA"
                     /locus_tag="ACHOQW_003009"
     CDS             255..1352
                     /gene="mltA"
                     /locus_tag="ACHOQW_003009"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP9574054.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1591..2397
                     /gene="tcdA"
                     /locus_tag="ACHOQW_003010"
     CDS             1591..2397
                     /gene="tcdA"
                     /locus_tag="ACHOQW_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP9574055.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2448..2891)
                     /gene="csdE"
                     /locus_tag="ACHOQW_003011"
     CDS             complement(2448..2891)
                     /gene="csdE"
                     /locus_tag="ACHOQW_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP9574056.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2891..4096)
                     /gene="csdA"
                     /locus_tag="ACHOQW_003012"
     CDS             complement(2891..4096)
                     /gene="csdA"
                     /locus_tag="ACHOQW_003012"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP9574057.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4288..4515
                     /gene="ygdI"
                     /locus_tag="ACHOQW_003013"
     CDS             4288..4515
                     /gene="ygdI"
                     /locus_tag="ACHOQW_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP9574058.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4866..5783
                     /gene="gcvA"
                     /locus_tag="ACHOQW_003014"
     CDS             4866..5783
                     /gene="gcvA"
                     /locus_tag="ACHOQW_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP9574059.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5802..6197
                     /gene="ygdD"
                     /locus_tag="ACHOQW_003015"
     CDS             5802..6197
                     /gene="ygdD"
                     /locus_tag="ACHOQW_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP9574060.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6190..7290
                     /gene="rlmM"
                     /locus_tag="ACHOQW_003016"
     CDS             6190..7290
                     /gene="rlmM"
                     /locus_tag="ACHOQW_003016"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP9574061.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPNRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7334..8065)
                     /gene="fucR"
                     /locus_tag="ACHOQW_003017"
     CDS             complement(7334..8065)
                     /gene="fucR"
                     /locus_tag="ACHOQW_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP9574062.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(8123..8545)
                     /gene="fucU"
                     /locus_tag="ACHOQW_003018"
     CDS             complement(8123..8545)
                     /gene="fucU"
                     /locus_tag="ACHOQW_003018"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP9574063.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8547..9965)
                     /gene="fucK"
                     /locus_tag="ACHOQW_003019"
     CDS             complement(8547..9965)
                     /gene="fucK"
                     /locus_tag="ACHOQW_003019"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9574064.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10074..11849)
                     /gene="fucI"
                     /locus_tag="ACHOQW_003020"
     CDS             complement(10074..11849)
                     /gene="fucI"
                     /locus_tag="ACHOQW_003020"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP9574065.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11882..13198)
                     /gene="fucP"
                     /locus_tag="ACHOQW_003021"
     CDS             complement(11882..13198)
                     /gene="fucP"
                     /locus_tag="ACHOQW_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9574066.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3263 a         3339 c         3543 g         3339 t
ORIGIN      
        1 cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgtccg tggatgcgca
       61 ctatactccg ctcgcgtttt catgcaacct tttttttacc ccgatcacgg atctggcatg
      121 attttgaaca aaaacaaatc actaaagcct attttttgtg caaaatttgc gtcagggaga
      181 ggctgctcat ttcattaaac gcggtggttt gttatcttcg ttgcgcctta ttttttaacc
      241 tgaagaagag aacaatgaaa ggacgttggg taaagtacct tcttatgggc acggttgtgg
      301 caatgcttgc cgcctgctct tccaaaccaa ccgatcgcgg acagcaatat aaagacggga
      361 aatttaccca gcctttctct ctggtgaacc agccagatgc cgttggcgcg ccgattaacg
      421 ccggtgattt tgccgagcaa attaaccata tccgtaattc gtcaccgcgt ctgtatggca
      481 accagagtaa tgtttataac gcggtgcaag agtggctgcg cgcaggcggt gatacccgca
      541 atatgcgcca gttcggcatt gatgcctggc agatggaagg tgccgacaac tatggtaacg
      601 tgcagtttac aggctattac acgccggtaa ttcaggcgcg ccatacccgc cagggcgagt
      661 tccagtatcc tatttaccgt atgccgccaa aacgtggtcg tctgccgtct cgtgcggaga
      721 tctacgcggg tgcattgagc gataagtaca tcctcgctta cagcaactct ctgatggata
      781 atttcattat ggatgtgcag ggtagtgggt atatcgactt tggtgatggc agtccgctta
      841 actttttcag ctatgcaggg aaaaacggtc atgcctatcg cagcattggt aaggtgctga
      901 tcgaccgtgg cgaagtgaaa aaagaagata tgtcgatgca ggcgattcgt cattggggcg
      961 aaacacacag tgaagccgag gttcgcgagc tgctggaaca gaacccgtct ttcgttttct
     1021 ttaaaccgca atcttttgca ccggtgaaag gggcaagtgc ggtgccgctg gttggccgtg
     1081 cttctgttgc ctctgatcgt tccattatcc caccaggtac aaccttgctg gcagaagtgc
     1141 cgctgctgga taataacggc aaatttaacg gtcagtacga attgcgtctg atggtggcgt
     1201 tggatgtcgg tggcgcaatc aaaggccaac acttcgatat ttatcaaggg atcgggccgg
     1261 aagccggaca ccgcgcaggt tggtacaacc actatggacg tgtctgggtg ctgaaaaccg
     1321 ccccgggcgc aggtaacgtc tttagcggct gatgtgatat tctgtatgcc gtttttacgg
     1381 atatgacagg gtgaggggta acctcacatc ctgattattg actaagtgcg cgttgttcat
     1441 gccggatgcg gcgtgaacgc cttatccggc ctacaaatca tgcaaatcca aaatattgca
     1501 ggagttatgt aggcctgata agcgtagcgc atcaggcatt tatgcgttta gggtgaggga
     1561 taacctcgcc ctttttaatt ctgaggtgct atgtctgtgg taattagtga tgcatggcgt
     1621 cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact gtttgctgac
     1681 gcgcatattt gtgtggtcgg tattggcggt gtcggttcct gggcggcgga agcgctggcg
     1741 cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt caccaatacc
     1801 aatcggcaaa ttcatgccct gcgcgataac gttgggctgg caaaagcgga agtcatggcg
     1861 gaacgtattc gccagattaa ctcggagtgc tgtgtaacgg tggtggatga tttcgtgacg
     1921 ccggataacg tagcgcagca tatgagcgtg ggttattcgt acgtgattga tgccattgat
     1981 agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat cccgctggtc
     2041 acgaccggtg gcgcgggtgg gcagattgac ccaacgcaga ttcaggttac cgatctggcg
     2101 aaaacgattc aggacccgct ggcggcgaag ttgcgtgagc gcctgaaaag cgattttggc
     2161 gtagtgaaaa acagtaaagg taagctcggt gtggattgcg tgttctctac cgaagcattg
     2221 gtgtatccgc agtcagacgg tacggtgtgt gcgatgaaag ccacggcgga aggaccaaag
     2281 cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac ctttggtttt
     2341 gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca ggggtaaaca
     2401 atcaggtcgg atgcgacgca aacgtcttat ccgacccggt tctgtgctta aacctgcttc
     2461 gtcgcagcga taatcgcctc gcttaacgca tttaacccct ggctgcgtga ggcgctaagt
     2521 tgcgcacgca atcccagctc atcaaacaat gccagcggcg actgtgcctg caattcggcg
     2581 gcggttttcc cctcaacggc agtcaacagc accgccagga ggccgcgcac tatacgtcct
     2641 tcgctgtcgc caaagaagtg cattttaccg ttttcagcca cggtatatcc cagccagacg
     2701 cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg caaagccgga
     2761 agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcgt caacggtgcg
     2821 aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc ggcgaattgc
     2881 gggttcgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc accagcgcat
     2941 ccacatcact ctttgtatta tatggcgcaa aagaggcgcg cagtgtgccg gtcacgccta
     3001 attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata ccgtactccg
     3061 ccagcagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc agcaggctgg
     3121 aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc cagcgcatct tccgccagcg
     3181 ttgctaagct acggctccag ttttcggcct ggttgatatc gtaatctgcc agccattcca
     3241 gcgccgcgct taatcctatg acaccagcca catttggcgt tcctgcttcc agtttccacg
     3301 gcgcagattg agtcgtgaag ccgtcaaaac tcacttcgtg aaccattttg ccgccgccca
     3361 gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg atacccgtcg
     3421 ggccatacag tttgtgacca gagaaagcat agaaatcgat atccagttgc tgaacatccg
     3481 cggggaaatg caccgccccc tgagcaccat caactatcac cactatcccg gctgaatgag
     3541 caaaggtaat cgctcgcgcc agatccgggc aaccgccagt tacgttcgac atctgaccca
     3601 acgccagaat ccgactacgg ggagtaatca gttccggcag caaatcgaca tctggcagtc
     3661 gctgcgcatt aagcggcaat ttgaccactt tggctccagt ttgttgggcg accatcagcc
     3721 aggggacgag gttggcgtgg tgttctgcca cgctgacaat aatctcatcg cccggttgca
     3781 gacgcggacg cgcatagcat tgtgccacca tgttgatgga ttcagtggtg ccgcgcgtcc
     3841 agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct cgtgcagctt
     3901 cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg tttccggcgc
     3961 tcagactgta aaactgtcgg gtggcttcaa ccacggcttc aggtttaagc gcggtcgcgg
     4021 cgctgtcgag atagacgccc gcatcctgta gagcgggaaa ctgggcgcga aactgcgcgg
     4081 gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc aaaattcact
     4141 ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga agccgcgtca
     4201 gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat actattaaga
     4261 tttattaatc gctttaagga gaagatgatg aaaaagactg ccgcaattat ttctgcctgt
     4321 atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca caccaatgac
     4381 ggacgtacca tcgtctctga cggcaaacca cagactgata acgataccgg tatgatttcg
     4441 tataaagacg ctaatggcaa caaacagcag atcaaccgta ccgacgtgaa agagatggtc
     4501 gaactggatc agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt aggcggtgct
     4561 acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc
     4621 taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca caaccgtaag
     4681 ccaaaagttc accagaacac gcattccgat aaaacttttc gttccggctc aggaagtgcc
     4741 gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc tcagattaaa
     4801 aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat caaagtttaa
     4861 aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat gccgcagcac
     4921 gccatttaag tttcactcgc gcagcagaag agctttttgt gacccaggcc gcagtaagtc
     4981 atcaaatcaa gtctcttgag gattttttgg ggctaaaact gttccgccgc cgtaatcgtt
     5041 cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag atattttcgc
     5101 aattaaccga agcgacgcgt aaactccagg cccgtagcgc caagggggcg ttgacggtca
     5161 gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc tttaattcag
     5221 cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat aagctggcgg
     5281 atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta cgggtggaaa
     5341 aactgtacgc cgaatattta ttgccggtgt gttcgccgct actgctgact ggcgaaaaac
     5401 ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg tcacgccgtg
     5461 actggcagac atatactcgt caattggggt taaatcatat caacgttcag caagggccaa
     5521 tttttagcca tagcgccatg gtgctgcaag cggctattca cgggcaggga gtggcgctgg
     5581 caaataacgt gatggcgcaa tctgaaattg aagccggacg tcttgtttgc ccgtttaatg
     5641 atgttctggt cagtaaaaac gctttttatc tggtttgtca tgacagccag gcagaactgg
     5701 gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa caagaaaaat
     5761 tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc cgttttatgc
     5821 tgattttcgc cgccattagc ggcttcattt ttgtggcact gggcgctttt ggcgcgcatg
     5881 tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccagaccggc ctcgaatacc
     5941 aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt cgcatcagta
     6001 tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc agcggcagcc
     6061 tttattgcct ggcactgtcc catctgcgtt tgtgggcgtt tgtcactccg gttggcggcg
     6121 tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta aagcgcaagg
     6181 gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc
     6241 gcagaaatta ccgataaagc cggccagcgg gagattttcg gttttgcccg cgtgaaagag
     6301 aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa gttaatccgt
     6361 gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg ggaactcttg
     6421 cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttgca gggcgtagta
     6481 gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa agagttgctg
     6541 aaattctgcc gtaaattcac cgtgccgcta cgcgctgcct tgcgcgatgc cggggtgctg
     6601 gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc accaggctgc
     6661 tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg cattccgcgc
     6721 ctgaaatttc cggcagatgc gccgaatcgt tccacgctca aactggaaga ggcatttcat
     6781 gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg ggcggtggat
     6841 ttaggcgctt gccctggcgg ctggacctac caactggtga agcgcaacat gtgggtttat
     6901 tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccggacaggt gacgtggctg
     6961 cgggaagacg gtttcaaatt ccgtccgacg cgcagcaata tctcctggat ggtatgcgat
     7021 atggttgaaa aaccggcgaa agttgcggca ttgatggcgc agtggctggt taatggctgg
     7081 tgtcgtgaaa ccattttcaa cctcaaactg ccgatgaaaa aacgctacga agaagtgtca
     7141 cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc tcagattcag
     7201 gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg tatctgggct
     7261 gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg tctccggcct
     7321 gctacccttt ccgtcagcct gttaccaaag aagttgcaac ctggcgttca tcagaaataa
     7381 tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta aatttacttt
     7441 tatcaatcaa taacaacgat tgcgcggcgc gttttaatag cattgatttg taatcagcgt
     7501 tgatagcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa
     7561 acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg taacagccat
     7621 atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt ttaccgagtt
     7681 catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc aactggcgtg
     7741 ccagatacca gcaagttgaa ctggcgtcta aggctatcac catcccttct tcaatccacg
     7801 cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc ctgatgtgaa
     7861 agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca tgattgcgca
     7921 gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct ttactgacct
     7981 ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc aggtcgacta
     8041 tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac cgcccgcgtc
     8101 atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga atattcccgt
     8161 acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg
     8221 caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc gcattacggt
     8281 aacgtcgttc tacttcagga tcgagagtgt caccttctac cgctgccatc atcaccagcg
     8341 gcggtgcata actgtccagc tcaaataacg ggataatcgc ctggagcaag tcgctcacca
     8401 acaggccatc agcgcggatc acctgcggtc ccatcgaatg ggcgggaaag tgagcatcgg
     8461 aaaaaataat ttcatctcca tgtcccatct ctgccagcac tttcaatagt tcgggagaaa
     8521 ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg ttcagtttgc
     8581 gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg gctgttaaat
     8641 tcccctacgc cataccaacc gaacagcgca gctcctgcga cggtcgtttc ggcgtcgtcg
     8701 agaactttta acggaatatc aagcatattg gctttaatct gattccacaa tgtgttgcga
     8761 cttcctccac cgactaacaa taattcagag gccttaaagt gcccgatttt ttccagcatc
     8821 tgtagattgc gctgtaattg cgcagttaac ccttccagcg ccgcgcgata gaaatgcccc
     8881 cgcgtggtat taagcgtcac tccttgccag ccagcgttct gacacgacaa taaatcacac
     8941 tgcattttca cgccatccgc gccaggcgcg atcagacgag cctcttcaat caacatttgc
     9001 cagggtgttt cagccgtcca gaacagtttt ctcacccatt ccagcacacc ggatgccagc
     9061 cattgcatac ctgggttata caaccctgcc tggctatcca gttcgcaggt ggaaccggcg
     9121 tactgactta acagcgaagt atcaacctgg gcgctgcgaa ccattaaaat ttcccatgta
     9181 ccggaagaga gcacgggttc attttgttca gcaccagcgc caaaaagggc gaactgggta
     9241 tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc tgcggcgctg
     9301 ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag tcggcgtgga
     9361 ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc cagcatctgg
     9421 ctggttccgg ccatcgtgat atcagtagtg aattcgccgg ttaaacggtg gttaatcagc
     9481 gacgaaataa agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac
     9541 cacaccaact tatataacgt attgaaacta aaggctccga cgccagaaat agcctgcaac
     9601 cgctgtgcgg agattaaccg ttcaatattg tccataaccg ctgctgttcg cggacatttc
     9661 cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc atccacacca
     9721 aaggtggtga cggcgatacc gcggatgtgg cattcagtca gttcactatt gatttgccga
     9781 cagcaatcag caaagcgttg caaaatggcg tctaaagacc actggtgcca ggtgttgttt
     9841 tccatcgcga tatcgctggc attaggcgtt gaggcgcggg caacaatttt tccctgccga
     9901 ttaaccgcga tggccctgac attggtcgcg ccacagtcga gtaccaggat cacttcttgt
     9961 ttcatgatcg ctcctgcaat atagccggat aacattgctt atccggctaa ccactcttgc
    10021 ttgttcaggc ccggcagccg gagcgaccgg gcatcacatc agggagtaat gtattaacgc
    10081 ttgtacaacg gaccgtagtt ctggcaagcg cggtaatcct ggccttcaat atccatgccg
    10141 tgcgcagccc aggcagaagg acgatacact ttggtctctt caacgttgtg catacatacc
    10201 gggatacgca gcatggaggc gagagtgata aagtctgcgc caacgtggcc gatggtcaga
    10261 accccatggt tagcgcccca gttcgccatt accgagtaca catccgtaaa cggcccttta
    10321 ccggtgaggc gcggtgcaaa ccaggtggtt ggccaggttg agttggtgcg tttgttgagg
    10381 atgtcatgca catccttcgg caattccacg ctccagcctt ccgcgatttg cagtaccggt
    10441 cccaggcctt tgatgatgtt gacacgagtc atggtgaacg ggacgccgcc ttcggtaagg
    10501 aagcgggaag agtaaccgcc gccacggaag tattcgtgga tcgccgggca ccattcggta
    10561 gcggcgaggc aagcgtcagc ctcttgctga gagatttccc agtgcggctt catcgtcggg
    10621 ttaccttcgc tgtcgcgttg tttacaggaa ccgtccagcg cagcagaacc ggagttgatc
    10681 aaatggatga tgccgtgttc tgccagtcca tccagtttat gccccgttac acgctcaatt
    10741 gcttctggtg accagtaggt acgcacatcg gcaaatacct gagcggtgcc ggtgagctgg
    10801 tgacccatta gcattgccac gccgttaaga ctgtcgtttt cggtcgccac gacaaagggt
    10861 tcgcgcacgc cattccagtc aaatgaactg ttgaggatcg cttcggcggt gtcaccattg
    10921 ggatattgat cggtccagtg acgttgcccc tggaagcccg cagcgatggc gttgtagcca
    10981 agtgattctt ccacgcgacc aatatcggcc agtttgctgt tgccttgcat catgtcgcgg
    11041 atacacatcg ccatcagtaa actttcgcgc agaactgcgc ggctttgctc ggcattgcgt
    11101 tgatactgtt tgttattttc atcttcgcca tagcggaagt ttttatcagc ccaggccagt
    11161 gccatttcca gttcggcttc gtcgtaaatc ttctgatcga tacggcgacg cagttcggtc
    11221 atatccaccg cctggacttt cattcccagc caggattcaa agaagttgtg atcaacaata
    11281 gaaccggcga tacccatcga aacgccgccc agcgacagat agcttttacc tttcatgctg
    11341 gcgacggcca aaccggcgcg ggcaaagcgc agcagttttt cttcaacatc ggcaggaatc
    11401 gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg agaatgccgg gatgcctttc
    11461 tggctgtgag ctgccagagc cgctgccagg taaacagcgc cgggacgttc agtgccgtta
    11521 aagccccaaa tggctttcgg gcgggttgga tccatgtcga tggtttcact gccatagcac
    11581 cagcaaggcg ttaccgtaat ggtgaggcct acattctggc tgctgaattt ttcttcgcaa
    11641 gcagcggctt cagccatacc cgcgatacag gtatcggaaa tgacacactc gacggcagct
    11701 ccgcaggcat ggcgcagttt ctcggtcagc agtgcggccg tagctttcgc catattcatt
    11761 gtttgttctt caagcgactc acgaacaccc atgcgacgac cgtcaataac cgggcggata
    11821 ccaattttcg gtaagctgat tttgttcatt acagattcct cactttattc ggaaaatatg
    11881 ttcagttagt tgccgtttga gaacggaaac gggcaaagat aaagatgacc gcgaagcaga
    11941 gtgcggggat cagttcagca gtggggatgt tgcccgccgc gtcactgaca aaacccatga
    12001 ccggagtgac aataccgccg ccaatgatgg tcataacgat gaaggacgaa ccgtatttgg
    12061 tgtcctggcc gagattctta atgcccagcg agaagattgt tgggtactga atcgacataa
    12121 aggcgctgca taaagtcagg gctattaagc ccacatgact gccagcgaag gctgagatca
    12181 ggcacagtgc catagcgatt aatgcgtagg cggccaggac tttgtgtggt gcgaagcgac
    12241 tgatgagcca ggtaccggtg aaacgaccaa taaagaagca caccatggtt ccggttaaat
    12301 agttagcggc aaagcctgca gtcatgcctg gaatttcttc tacagcgtag cgaatcaaat
    12361 agctccagca ggccgtttgt gcgccaacgt agcagaattg cgctaatact gcccagcgcc
    12421 agtggcgaat acgcgccagg cgagaaagcg atgcggagaa cgatccttgt ttggcgtcac
    12481 tgtgattatc actctgcaat gccgggaatt tcgtcagcat gatcagcagg gcgaccagta
    12541 acacgatagc cacgatgatc atataaggtg tctgtaccga taataccagg ctgtgtttat
    12601 acgcactcaa ttgctctgga gacattttat cgagaacgtc ttgcgattga tgtggcacgt
    12661 tagacaaaat aagactttgc ccaaagacaa ccgcgataat tgcgccaaat gagttaaatg
    12721 tttgcgcaag atttaagcgg aagtgaccac tgctttccgg ccctaatacc gtaacaaaag
    12781 ggtttgcggc agtttccaga caacctaatc cggctgcaat aataaatagg ccaactaaaa
    12841 ataaggtgta gttcattatt tctgcggcgg gccagaataa tgcagcaccc aaggcatata
    12901 aaaataatcc ggtaataatt cctgctttat aactgagttt tttcatcaat atcccagcag
    12961 gaattgggat aatgaaataa ccaaagtaaa aggccgattg gatcaggcca gcctggaaat
    13021 ttgtcagtgt aaaagcctgc tggaattgag gtaataaaat gtcgttaagg ttattggcta
    13081 ccgcccaaag aaaaaacagt gagcacagca gcgcgaatgg aataatgtaa cttctgcttt
    13141 gccctgcatc tttatctacc gcacggtaac tctgcgtttg tattgatgtg tttcccatag
    13201 catcctctta ggttcagaag cttaagcacg taatcggtta tgttttcaaa agtgcaaaac
    13261 tagcgagttc gggcttcata tcagtgaata tttgcctgtc tgaaatgaac tcatggattc
    13321 attttcaaat aaaaaacgct ctgtaatatg acggcggtca cacttattag ctggaaggtg
    13381 actttatgtg actaccatca ctttaataat cgaaatttca tgcccgtttc agaaaataaa
    13441 tcataaaaaa cacaaaccag atgaccgtat taattgttta tgcc
//
LOCUS       DAZKFZ010000114        13456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000114 DAZKFZ010000000
VERSION     DAZKFZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            113..1171
                     /locus_tag="ACHOQW_003022"
     CDS             113..1171
                     /locus_tag="ACHOQW_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFP9574067.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1181..2323
                     /locus_tag="ACHOQW_003023"
     CDS             1181..2323
                     /locus_tag="ACHOQW_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFP9574068.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2316..3089
                     /locus_tag="ACHOQW_003024"
     CDS             2316..3089
                     /locus_tag="ACHOQW_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574069.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3091..4170
                     /locus_tag="ACHOQW_003025"
     CDS             3091..4170
                     /locus_tag="ACHOQW_003025"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFP9574070.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4170..5126
                     /locus_tag="ACHOQW_003026"
     CDS             4170..5126
                     /locus_tag="ACHOQW_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFP9574071.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5137..6345
                     /locus_tag="ACHOQW_003027"
     CDS             5137..6345
                     /locus_tag="ACHOQW_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFP9574072.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6363..6830
                     /gene="terW"
                     /locus_tag="ACHOQW_003028"
     CDS             6363..6830
                     /gene="terW"
                     /locus_tag="ACHOQW_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFP9574073.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7091..7420
                     /locus_tag="ACHOQW_003029"
     CDS             7091..7420
                     /locus_tag="ACHOQW_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9574074.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7407..7748
                     /gene="tnpB"
                     /locus_tag="ACHOQW_003030"
     CDS             7407..7748
                     /gene="tnpB"
                     /locus_tag="ACHOQW_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9574075.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7830..8660
                     /locus_tag="ACHOQW_003031"
                     /pseudo
     CDS             7830..8660
                     /locus_tag="ACHOQW_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8691..10304)
                     /locus_tag="ACHOQW_003032"
     CDS             complement(8691..10304)
                     /locus_tag="ACHOQW_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFP9574076.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10335..10685)
                     /gene="tnpB"
                     /locus_tag="ACHOQW_003033"
     CDS             complement(10335..10685)
                     /gene="tnpB"
                     /locus_tag="ACHOQW_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9574077.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10682..11107)
                     /locus_tag="ACHOQW_003034"
     CDS             complement(10682..11107)
                     /locus_tag="ACHOQW_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9574078.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11161..11912
                     /locus_tag="ACHOQW_003035"
                     /pseudo
     CDS             11161..11912
                     /locus_tag="ACHOQW_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            12252..12425
                     /locus_tag="ACHOQW_003036"
     CDS             12252..12425
                     /locus_tag="ACHOQW_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574079.1"
                     /translation="MSSPEQYLSSSEPASDTSLAVAPAQQNKSTIVRLRSFVCIADDA
                     PEKRVNMSSPEYY"
BASE COUNT         3245 a         3541 c         3504 g         3166 t
ORIGIN      
        1 actccataac cacaggagtt attaatcata ttatgattga catgtagctt agacactcat
       61 agactcactt ctcaatcata aatttgcatg gcgttccgga taattaaaaa aaatgaacaa
      121 gaaaatttgg tttatggaag gtttatcctc ccagcgagat attattcagg gggtaaaatc
      181 atttgcacaa aaaaataatt ttgccattac cgtttttgcc tcccaccgta acgaaagaaa
      241 tgaaatcctt tccgttgccg attattcttt gactgaacct gaagatcctc aaaaacgtct
      301 tcagtttatc caggaaacca ttcagaccta cggcatccac catattcata ctggccgtaa
      361 cagccagtgg tttgaagaac accgttcagc cattgaaccg accggtgcca cccttactac
      421 cggtgcaacg ggcgtcgact ggttaactct ggctgacgaa aaagttactt ttgctcagtt
      481 tatggagcaa aagggtctcc cggtcgtacc atcctggcgg gtgaatacgc tggcagaatt
      541 aaagacacac ctcgcggccc cgccgttcac tgacagcccg gtatgcgtga agccggtgac
      601 gggtatctat ggcatgggat tctggcgctt tgatgacagt gcttcgccta tggccgtctt
      661 taatcatccc gaacatcgtc tggtcagtcc gcaacagtat attgcagcag catcagctgc
      721 tgagtcgttt aaaccccttg ttttgatgcc gtacctgcca ggcccggaat tttccgtcga
      781 tatcctcgcg gataagggcg aaatactcgc agccgtggga cgccgtaagg aaggggctat
      841 ccagtatctg gtaaacgaag gaagcgcctg ggaactggcg tgtgactgcg cccgtgttat
      901 gaaggccgac gggctggtga atgttcagac gcgaaacgat gtgaatggca acccggtgct
      961 gcttgaaacc aacatgcgtc cgtcaggggg ggtgggttat acccttcaca gcggtgtgaa
     1021 ccttcctggg ttatttgctg cctttaagct cggtctgatg tctgaagata tggtacgcca
     1081 gagcgctaaa aacacctttt ctccggttgc ggtgagatcc attacggatg taattgcata
     1141 cccggaatca ctctctaacc ttctgaatta aggcgtattt atgtctgata tcctcgaaga
     1201 tatgatttat cgccgtaccc tctcatgcgg tacgattcag gtaacccgtg accagggtga
     1261 agtttcgctc gatgacctgt ttgacatcgc tgaaagacgt aacccgaaac gcgcctttct
     1321 gtttgtcagc aaagtactcg gcaggcacat tcccgtgcca ccctcagtca tgcggcaggc
     1381 ctacagacag cttgccagcc agttcccctc gacactgaca ggacccgtac tgtttatcgg
     1441 tatggctgaa accgccgttg ggcttggggc cggtgtattt gatgaagtgc gccaccagca
     1501 tcgtgaatct gtctatctga cctctacccg tcacccggtt gatggcacgt tgctttgcga
     1561 gttcaaggaa gaacacagcc acgccaccga tcatctgatc tatctgccag atgatgaaga
     1621 gaaaagacgt cgtgttacca acgcacgaac gctggttttg attgatgacg aggcaaccac
     1681 cggtaatacc tttattaacc tgctttctgc cctgcgtaat accggcaagc ttcaacatat
     1741 tgaacaggtt atcgccgtaa cgctgaccga ctggagtggc aatgccctgt cggagcgcac
     1801 ccccttgccc gtcacttcgg tttcgcttgt aagcggtcag tggggatgga ccccattacc
     1861 tgatgcacct gtcccggaca tgccgaaagt caacgtaact tcacgaggag aatgggacat
     1921 ccaggggaaa cagtcatggg gccgactggg gatgctcgca cctgcggccg atctcggcca
     1981 tgaggtctcc gtccacaagg gggaacgtat tctggttctc gggaccgggg aattcgtctg
     2041 ggagccgttc ctgcttgctg aacggctcga agctgccgga gcacaggcat tttatggatc
     2101 gaccacccgc tcccctgtcg ccgttggtta tgccattgag tccgccattt cctttacgga
     2161 taactacggg ctgggcatcc ccaattttgt ctataacgtc gcccaccagc agtttgaccg
     2221 cattcttgtg tgtactgaga cacccgcaga aagtattgac acgcagcttc ttaaagcgct
     2281 ggctgaggtt gcgcccgtcg tggagattgt tacctatgaa taaacccgtt gttctgagcg
     2341 accttgacga cacgctcttt cagacccgtc gtaaaatggt ggacgagctg gccctggagc
     2401 cattccgtac cggtgccgtt gatcgcaccc tgaatccacg aagctttatg acggaagaac
     2461 agtccatgtt ggtggactgg ctcctggagc aggctgaact catacccgtt accgcacggg
     2521 gaactgaaga aatcagccgc gtacggatcc ctttccactc ctgggcaatc acaacgcacg
     2581 gggccgtcat tctcacgcct gaaggcaaac ccgacgagga gtggaaggcc catatgctcg
     2641 gccagctggc tccctaccag gaaaagctga catcgatgca gcgtctgatc actgaaatga
     2701 tggacgcaaa aggaatcaat gcctgggcaa ggctgaactt cgaatacggt gaaacggcgg
     2761 tttatatggt gatgaaacac cgcgacagca cccgccttga cgagctcaat gccattgcag
     2821 atgagataga aacggtgttt ccgaccgagg gcttctacat ccaccgcaac agtaataacg
     2881 tggcctggct tcccaccccg gttgagaaag ggctggcagt cagctggctg cttgaaaaac
     2941 ttcgggctga acgaggagtt ttccccgtaa ttggtttggg cgacagcctg agcgatcatc
     3001 gttttatgaa actgtgcagc tggtttggca tcccgcgtca gagtcagttt gcagatgcca
     3061 tttcacagcg aatttttgga gaaaattaaa atgcagccac atgaaacatt taccggctca
     3121 taccagcccg gtgacgtgga atttctgcta aagccggttg tcattgagat gacgccggtt
     3181 gagaaaaaag aagaactgat tcagtccggg aagaaacatt attcggacat gctcagccag
     3241 gagccagcgc ctacacaatg gcatcttgat ttgtttcacc gggcgctgga tcggggcgca
     3301 gagaggcttg caaaagaagt cacacagctc gctattgctc tggccgaacg cttcggtgat
     3361 gagcccattg tactggccag tcttgtcagg gctggcgttc cgctcggcgt tatgctgcac
     3421 caggccctgc gtgacatggg gaaaacctca tggcattacg gtatcagcat tatccgggat
     3481 cgtggaattg acggtgcagc cctcgacgtc attgaagagc ggcatggtac cagcggtatt
     3541 gtctttgttg acggatggac aggtaaaggc gcaattaccg gagagcttgt acgcgcactg
     3601 aaagatcgcc cgggctatcc tgagcagccg cgactggttg tcctggccga tccctgtggc
     3661 tgctcctggc ttgcagccag tgatgatgac tggctcattc cttttggcat catgggggcg
     3721 ccggtatcag gcctgatctc ccggtcagta tggtcctctg aaggattaca cgggtgcatg
     3781 gtttgcgagc atctcagtga attcgaatgc agccggatgc ttgccgatac cgtcgctcat
     3841 ttccgtaaga agttaacacc gtcatccctc gctcccctga gctggaatac ggaatcagcc
     3901 cgggtcttat ggcagacaag tcgcgacgtt atcgcgttcc tggccgatga atttaaagtg
     3961 gacagcgtca atcgtattaa acccggtatt gctgaagcaa cccgggctgt attacgtcgg
     4021 gtaccggacc atgtatttgt gcgttctatt gacgacccgg acgttgcctt gcttgtaggg
     4081 cttgctcgtg aaaagggaat agttgttaca gaaatggggg gaaccctcgg ccagtatcgg
     4141 gctgtaacca ttatcaagaa ggtactctga tgaaaaatcg tctttctccc tggaatctgg
     4201 gagccacgct atacatgcct gcaacacggg aagatattgc tgatgccgtc ctgcacggga
     4261 aaatcccggg cttgcgctct ctggtgattt gcctggagga tgctgtcagt gaagcagaca
     4321 tccccgtcgc cctcaaaaat ctggagcacc tgctgcacga gctcagtaac agcatgagta
     4381 gcctgggtaa aaatgactgg cctctggtgt tcattcgccc ccggcatgcc gaaatgggca
     4441 gatggttaaa agcgcattat gatctttccg ctgtcgacgg gtttgtgctg cctaaattta
     4501 ccctcagttc gttagctgaa tggtgggata tcatggtcgg gactcacctg tgcatgatgc
     4561 caacgctgga aacagaagat gtctttgacg tggttcagat gcgcgagctg gctacccgtc
     4621 tggaggaaca tccctgccac gaccgcatta ttgcgcttcg aatcggcggc aacgatctta
     4681 tgaacgttgt gtcgcttcgc cgtccccggg acctgacgct gtatgacagc ccgatgggct
     4741 acgtcatcaa aatgctcgtt tcggtcttcg gcccgcgtga ttttgccctg accgcgcctg
     4801 tatgtgagca tattgacgat catgccgtta tggccagaga gctggctctt gatatggcac
     4861 acgggcttgt tgggaaaacg gccattcacc cgggtcagat agaggtcatt caaaacgcgc
     4921 tgatggtcac tcagggtgag cattctgacg ctctgcggat cctgaactct acccaggccg
     4981 tgtttaagtc gcagggagca atgtgtgaac ccgccacaca tcgccgctgg gcggctggca
     5041 ttctggacag ggctcgtttt tatgggttac agaacgagca aagcgctgat ggaatcagat
     5101 tacttaccgt gacccagcat cattaaggaa atcataatgg ggttcaggtt cagaaagtcg
     5161 atcaacatta ttcctggcgt tcgcctcaac ctgagtaacg gtgcaccgag cctgagtgtc
     5221 gggccgagag gtgcttccgt ttcttttggt agccggggga cctatgccaa tctgggcttg
     5281 cccggtaccg ggctgagtta ccgtacccgg cttgaccggg ccgcgcgttc cagaggtgaa
     5341 aaccggacgg caaccgaccc ggggctcaga caggcgcttg agcagaaagc cgctgaactc
     5401 atgtcagcgg taaccgcaat ccgtaatatt cacgagctga cgccggatcc aaaaacaggc
     5461 atcagctggg cagagctgga agcagtatac ctgcataaca gaacgtcgcc ttttcaggtt
     5521 ccggcaccgg tgcgtccaga aaagccagac taccttgtat tgccggaaaa gcctgccgag
     5581 agcgaaggca ttagtttttt gggtaaatgg tttgaatcgg aatcagctaa agctgagcgc
     5641 cacgccgaaa atcttcgccg gtggcagcag gagctgattg atgtggagcg tgagaatacc
     5701 cttcgacagc accggtacca gcaacaacgg acggcctggg ccgaacagta tgcaaactgg
     5761 aagtttgaag ctgaagaaca tgaaaaacgg ctcgccacgg ctcaggcaga tgcccggcag
     5821 cagttccgga cagacgccgc gtttttcgaa tcatacctgg cgggtgtgct ggcagaaact
     5881 gaatggccgc gtgaaacgct tgttgcattt gaagtaaagc cggagctatc agcagtcctg
     5941 ctggacgttg atttagctga gattgaagat ttccctgata agatttacgg cgttaatgcc
     6001 cggggaacgg agctgacgga aaaagccatg acgcaaaaag ccgtacgcga aaactatgcc
     6061 caccacgtcc atggctgctt gttccgcctg gtcggtatcg ttttacatac gctacctttc
     6121 gacaacgtga ttgtgtcagg ctttacgcaa cgggtcagta agcggaccgg ctatctggag
     6181 gatgagtata tcctgtcctg caaatgcact cgcagccaga tgtcgtcagt aaattttgca
     6241 ggcataaaac acattgatcc ggttgaagcg ttaggcgatc aaccggttat tcgaaagatg
     6301 agcagtacct tcatttttca gcctattgaa ccactaaccc tttaagcagg ctaaatctta
     6361 cgatgcaatt aaataccaga caggcccgga tttttaagct tgccaactta ctgggcaccg
     6421 gcaagcccgt ttccgctgcc gacattataa ccagtctgga atgctctgag ccgacattaa
     6481 cccgggccct caaagagcta cgcgagtcat attctgcgga gatcaaatac agcaaggcag
     6541 gtcattccta tcatctcgtt aatccgggcc agttggataa aaagaccctc cgccggatga
     6601 atgaagcgct cgcacagaat gctgaactga aaaccggtga gtccacaggg aaggtcgtac
     6661 tcgataagga taaaaaaaca gccgtgtcat tatcgctacg catgcggatt ctaagaaaaa
     6721 ttgaccgtct ggctgcgctg agtgactcga cccgaagtga agcggtagag aagctggctc
     6781 tgcactccgt tgatgaactg ataaaagaat acagcgctaa aaagtcctga cgctaaatct
     6841 cagaccgggg tgaaaactcc ggttttgtca tatttcacct ctgtattggc gtgatgcaaa
     6901 atggcatctg aatcgatcat ggtttggcat ccatttccga tcactgtttg gcatcacaat
     6961 cgatcacgat ttggcatgct tccgctcatt gattggcatc ctggtgtcgt tacggggaag
     7021 tcatcttcac agggatcttc agtcagttag cattaccgac atccattcac caacttaccg
     7081 gagataatca atgtcaaaac cccgatggac gcttgatcag aaaaaacatc acgttgccgc
     7141 atggcgcgcc agcggcctta cccgtgaaca gtactgtgaa ctttacgaca ttccgtttaa
     7201 atccctgcgc cagtggccgc aggatgtcgc aaaagccgaa aaacgagcca gagcaccaga
     7261 aattattcct gtttcagtat ctggctcttc cggaatgact gatggcagac cattgtcgga
     7321 tgagcctgtc actctcttcc tgcccggtgg tatccggatg tgctgccagc cttctcagtt
     7381 aaccgatgtt ttccgggctc ttcgccatgc tgacgcctga taacgtattc ctggtcgtaa
     7441 aacccgtcga tatgcgccgg ggtatcgaca cactgacaca gtacgtccag aacgaactta
     7501 acgctgcatg gcatgacggt gctgcttttg tcttcaccaa taaagtccgc tcccgcataa
     7561 aagttctccg gtgggacaaa catggcgtat ggctgtgtac ccgacgactg caccggggaa
     7621 gcttccgctg gccccggaaa ggtgatgcca catggcatct cacacaggat gagtttcact
     7681 ggctggtctt tggggttgac tggcaacagg tgaaagggca tgacctggca aaatgggtat
     7741 accagtaaga acccgaagat gctcagagca acgcaataac tcaatgaaat ataatgaata
     7801 tccatgcgtg atgtcatata atccaccgca tggatatctc gcttctcagt accaccagtg
     7861 accctgaaca gcttcgcgct ctggcaattg ccatggtgca aaaagtcatg gctgagaatg
     7921 cagaacttca gaaccgtatc cgcatcctgg aagagcagat gaagctcgca cgccagcagc
     7981 gtttcggtaa aaaatgcgaa tcgctggccg ggatgcagcg ttccctgttc gaagaggatg
     8041 ttgacgcgga tatcgcagaa atctcggcgc atctggacaa actgcttcct cagaccgggg
     8101 acgaagaaaa aacaacaaca cgtcctgttc gtaagccctt gccttcacct cttccccgtg
     8161 ctgaaaaggt tatcccgcca gctgaggaac gttgtcctga ctgtgatgcc cccctgcact
     8221 tcattcgcga tgaagtcagc gagaagctgg aatatattcc ggcacaggtc gtggtcaacc
     8281 gctatatccg cccgcagtac agctgtccct gctgtgagaa agtgttcagc ggaaaaatgc
     8341 cggcacatat ccttccgaaa agtgctgttg agccatcggt catcgcacag gttgtcatca
     8401 gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc cgaatggggg
     8461 tggagctccc cgtcagtaca atggcggata tggtcggtgt tgctggcgct gcactggccc
     8521 cgctggcaaa attgttacgc cacgagcttc tgacacggga tgttattcat gcagatgaaa
     8581 cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc tggctgtggg
     8641 cttacgtcag cggagaaaga gtaagcgtca acggagcacc gtattgacgc ttatttattg
     8701 gtgagtacta cgttccatgg caggagttca tcaactcggt tggaaggcca ttctggcagt
     8761 acgctcagga tatggcgcag atacgcttcc ggatcgatac cgttcagacg gcaggtgccg
     8821 atcagcccgt acaacagtgc tccacgctcg ccgccgtgat cgctgccaaa gaacataaag
     8881 tttttctttc cgagacagac tgcacgaagc gctctttccg cagcattatt atccgcctcc
     8941 gccagaccgt catcactgta ataacagagg gcatcccact gattcagtac atagctgcac
     9001 gcttcgccca gtctggattt tttcgacagc gtaccattct tctccaccat ccattcatgc
     9061 agtgacgtca gtaacacttt gcttcgctgc tgcctgacgg caagacgctc tgactccggt
     9121 aatccccgta tttcatcctc gatggcgtac agttcactga ttcgcttcag ggcttcttct
     9181 gccgtcgcac ttttgctgct gatgtataca tcgtggattt ttcgccgggc atgggcccag
     9241 cacgcaactt ctgtcagtgc accaccttca cgttctgcac tgaacagccg atcgtaaccg
     9301 ctgaacgcat ccgcctgcag gataccccgg aagggacgaa ggtgttgtac cggatgtttt
     9361 ccctgcctgt ctggtgagta ggcgaaccag accgccggtg gctctgatga gcccgcattc
     9421 cggtcatccc ggacatacgt ccagatgcgt cctgtttttg ccttttttct gcccggtgcc
     9481 agcactttta ctggtgtgtc gtcagtgtga accttgcggg tgttcatcac gtaacggtac
     9541 agggtatcat tcagcagagt cattaactga caacatgcgt caacccagtt ggagagtaat
     9601 gcccggctca gttcgatgcc ctggcgggca aagatttcac tctgacgata cagtggcagg
     9661 tgttcgcagt attttcccgt taacacgcgg gcaagtaatc cggagcccgc gatgccgcgc
     9721 tctatcgggc gggacggcgc tggcgcttca actatacagt cacattttgt acaggctttt
     9781 tttacccgaa cagtgcggat cactttcagg gcgctactca ccagttccag ctgctcagca
     9841 ctgacttccc ccagataatc cagctcactg ccacactccg ggcaacaact ttcttcaggc
     9901 tccagacggt gtatttcacg gggaagatgt gccggtaacg gacgacgatg acgtgactgt
     9961 cgcaactggc ggggaacctg cgggtcatcc tcacgcccac tgtaacgatc gctttcctgt
    10021 tcgcgttgtt tcagttgggc ctcagcctgt tcaacctcac gctgcagttt ttcagaacgg
    10081 gtaccgaaca acatccggcg cagtttttct atctgggccc tcagatgttc tatttcccgc
    10141 tcctcctctt cgatcttttc ctcggcacgt gccagtgcag agcgcaggaa ggcctccgtc
    10201 tcttcaacca gactcagttg ctgatctttc tgacggaggg cttcagcctg ctcagagagc
    10261 agcctttcca gctcagtgat acgaatgagg tatttccgac tcatgaccgt ttttataatc
    10321 cggccatgac atttttacaa cattgtcagt gcattaaggc gggatgtttt gggctgacgc
    10381 cagtccagtt tatcgaggag cattgccagc tgcgagcggg taatggatac cttaccgtca
    10441 cgcaccgcag gccagataaa ctggccttcc tccagacgtt tggtgaacag gcacagacca
    10501 tcagcatcag cccacagaat tttaatcgtg tcaccccgtc ggccgcgaaa gataaacagg
    10561 tgaccggaga aggggttctc atccagcaca tgttgtacct gttctcccag accgttgaag
    10621 gatttacgca tatcagtaac gccggcaacc agccagattc gagtgcctga tgggagcgag
    10681 atcatcgtcc cctcccggtc agttcacgga tcaacaccgt gagcagctct ggtgaaggat
    10741 tttccagcgt catgttaccg tggcggaact caactttaca ggaactggca ctgactgtgc
    10801 tttgtgaagg agtggataaa agcggagtaa gagccgccat aggctctttc tgctcatcag
    10861 gcgttatctc aacaggtaat aattcaacgc cagcgccaga agaggttgtt accggaagac
    10921 gccgcgatat acgcctttcg ttctgccaga gcctgagcca tttgaacagg aggttatcat
    10981 tgatatcgtg ttcccgggca atacgggcaa cagaggctcc tggttgtgaa gccagtttaa
    11041 ccatttgaag tttaaactca tttgaaaatg atctgcgggg ttctgaggat aatattttct
    11101 gttccataac aggtgtccac tagttgaaaa ggtgggcacc tacgttacca atactggctt
    11161 aatggctaca tacggcggtc agtttacgct tacgagaaag aagcggtccg ccggtcgtct
    11221 gttttgactc gcagaccgga cgggccctca gatatcctga ggcctggctg cagggctggt
    11281 gtggcggtac gctggtgagt gacggataca gcgtgtacaa atctctggcg gataatgacc
    11341 ccggtattac atctgcctgt tgctggagcc acgcaaggcg cggtttcgca aatctgtata
    11401 aggccagcag ggagccccgt gcagccatgg cgctgagaaa aatcgcgggt ctttatcgca
    11461 ttgagaagtt catccgtgaa cgtcctgtgg aaaaaatacg gcaatggcga caacggtatt
    11521 cccggccgat agtgaatgac ctgtttgcct ggcctgaaga gcaggagccg tgctgtccgc
    11581 ccgacggacc actaaacaaa gcgattaatt atatcctgaa ccggcgcgat gaactgagct
    11641 gcttcctgag tgacggagcc gtgcccctgg acaacaatat ctgcgagcgt gctatccgcc
    11701 cggtggtcat ggggcggaaa gcatggttat tcgccggttc actgatggcc gggaaccgcg
    11761 cggcacagat aatgagcctt ctggaaacgg caaaacgtaa tggtctggag ccgcatgcct
    11821 ggctgacaga cgttctgacg cgtctaccgg agtggccgga agaccgactg gaagagttgc
    11881 tgccccttga gggatttacc ttctccgggt gagtgatacc tgccatcagg tgttcgtgca
    11941 gcgggccata acccgcagtc gggagttgaa cgcctgacga caggaaatga gccagagcag
    12001 cgaccacgcg ggctgacagt gagtcagaac ctgaatcagc ccggagccat tcccggggct
    12061 gtacttcatc acctgccatc agtacccgtg tcagtacttc gcgacctgac gcaattaccc
    12121 gactcagttc ttcagaaccc gcctccgata ccagcctggc ggtcgcccct gtgcaacaaa
    12181 ataaatcaac catcgtcaga cggtgatcaa tcgtatgcgc tgccagtggc tcgcctgaaa
    12241 aatgcgtcaa tatgagttcg ccggaacaat acctcagttc ttcagaaccc gcctccgata
    12301 ccagcctggc ggtcgccccg gcgcaacaga ataaatcaac catcgtcaga ctgcgatcat
    12361 tcgtatgcat cgctgatgat gcgcccgaaa aacgcgtcaa tatgagttcg ccggaatatt
    12421 actgacgaca cggtttgtag ttacgttcct tcggtgagtg agtacctgtg cgtcagggta
    12481 acgcaatcag ttcaggttta gcggggtgtc aagtcggttt ggctaaccga ccgcagaatt
    12541 aactgtacct ttaaaggtcg cccccgatat atctgcctgt cagacatatc gccgattcgg
    12601 tatctcacta cataaaactc cttaaaaaac aatgcgattc tgttggtacg aagctttcag
    12661 taagtcagtc cggagaagcg ccagtacgat acagaatgag gttgaaaaaa acgttcacgt
    12721 ctggtcactt cttcaaatgt taaaaaacat ctcgtaaaga gccagtttat gcgggttcgt
    12781 tgagtagacg tgcgagcgtc actttgtcag ctttaacgct gacgaaagtg acgcccgcac
    12841 agcttcaaac tccacatgca cacgcaagcg accaaatttc aaatggtctt atgccgtgcg
    12901 cacagtcata agaagcgcat gcggaaagtc agaaaatatc ttaagacgca gattttaaag
    12961 gcgtttttat gccagtctgc cgggaataca cagtagtccg gttgtccgga agcaccacgg
    13021 taatccgaag gaaaaatcaa cgtacctcaa tcaccgtagt gatatccgtt cccagaggct
    13081 aaacggatgt accaccataa aggcatcctc tttcaggcta cgaggatatt gcaatcaccc
    13141 gaaggcgttt ctctcagatt gctgaaacat tggctggcag tcacccgaag gcgtccctct
    13201 cagactgctg gaacattggc tggtcgcaaa agcgacagtt aatgcaaata caggttaaca
    13261 aaaagggaaa tctgccgtca acccaaccat atgaacatgt tgatttcatt atgaatgtga
    13321 tgttctaata taaattaatc agtaacttca taaaaagaat ataagaaaca gataatgcat
    13381 gtatttacac acatatcagc acaatgatag attaccaccc cagaaacacg ataaaggtat
    13441 aattatggaa ttttcc
//
LOCUS       DAZKFZ010000115        13456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000115 DAZKFZ010000000
VERSION     DAZKFZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHOQW_003037"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHOQW_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP9574080.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /locus_tag="ACHOQW_003038"
     CDS             516..1217
                     /locus_tag="ACHOQW_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP9574081.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHOQW_003039"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHOQW_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP9574082.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1538..2848)
                     /gene="cyuA"
                     /locus_tag="ACHOQW_003040"
     CDS             complement(1538..2848)
                     /gene="cyuA"
                     /locus_tag="ACHOQW_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP9574083.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2876..4207)
                     /locus_tag="ACHOQW_003041"
     CDS             complement(2876..4207)
                     /locus_tag="ACHOQW_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9574084.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4482..5846)
                     /gene="tdcG"
                     /locus_tag="ACHOQW_003042"
     CDS             complement(4482..5846)
                     /gene="tdcG"
                     /locus_tag="ACHOQW_003042"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9574085.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5918..6307)
                     /locus_tag="ACHOQW_003043"
     CDS             complement(5918..6307)
                     /locus_tag="ACHOQW_003043"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP9574086.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6321..8615)
                     /gene="tdcE"
                     /locus_tag="ACHOQW_003044"
     CDS             complement(6321..8615)
                     /gene="tdcE"
                     /locus_tag="ACHOQW_003044"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP9574087.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8649..9857)
                     /gene="tdcD"
                     /locus_tag="ACHOQW_003045"
     CDS             complement(8649..9857)
                     /gene="tdcD"
                     /locus_tag="ACHOQW_003045"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP9574088.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9883..11214)
                     /gene="tdcC"
                     /locus_tag="ACHOQW_003046"
     CDS             complement(9883..11214)
                     /gene="tdcC"
                     /locus_tag="ACHOQW_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP9574089.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11236..12225)
                     /gene="tdcB"
                     /locus_tag="ACHOQW_003047"
     CDS             complement(11236..12225)
                     /gene="tdcB"
                     /locus_tag="ACHOQW_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP9574090.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12324..13262)
                     /gene="tdcA"
                     /locus_tag="ACHOQW_003048"
     CDS             complement(12324..13262)
                     /gene="tdcA"
                     /locus_tag="ACHOQW_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP9574091.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3520 a         3603 c         3156 g         3177 t
ORIGIN      
        1 cgcccccgcc agcacttctg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg
      121 cgtttcccaa ccgcgcgcga gagcttccgc atcagtagtc agtttatctg cggcttccag
      181 caaaacgcgc cctcgctcca acaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc tgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg
      781 aggcgttgct gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag
      841 acaaaccgtt aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa actggcgctt aatatgggca agcagcattt aatcgcctcg ccagagggtg
      961 cgatgggaag cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcacgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc
     1441 ggcctacaaa atccaacaac attaacgaat taaacaaccc gtaggccgga taagatgcgc
     1501 cagcatcgca tccggcacga tacccaaaac ctggcgttta tctggccttg ctcgccataa
     1561 tctcgataat ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg
     1621 caatcgactg ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat
     1681 ccagcgccat taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc
     1741 agctgttcga cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg
     1801 ccatcgagat ggtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca
     1861 ttgctgcggt cgttgcggca cacagcgcag acaaacgcgg taactggtta tggatgtaaa
     1921 ttgcgctcaa atgagaaagc atcagcgcac gcgccagccg ttcatcatcc gctccgaagt
     1981 gttctgctac caccaccacg ggcattgttg cggtgatccc ctggttaccc gagccggagt
     2041 tactcattgc cggaagcgtg gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa
     2101 tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg
     2161 tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta
     2221 gcttcgcgga atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca
     2281 ggatctcagc cagcgtcgtt ctggaaagca ccgatagcgg agactcttgc tcgccctctg
     2341 ccacacacgc ctgctgggta aacaccacac catcgtgcgt ctcaatatgc acaatgttgg
     2401 tatgcccgcc gacgatagtg acacacgccc acttctcacc gttccagact ttggcgcgtg
     2461 agaagaggat ttcattgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca
     2521 gtgctttggc atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt
     2581 tagcatttcc acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc
     2641 cgggaacggt gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac
     2701 gttcaaccgg accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg
     2761 gttcagtaca tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc
     2821 ataacggatt taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag
     2881 aaaatgccag gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag
     2941 acatcccttt gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc
     3001 accctaccaa gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca
     3061 gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat
     3121 tttcgttaat cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg
     3181 ttgcttcgcg aaaacctaaa tagacgccaa agaatgcagt catgacagca aagatattga
     3241 gaatgacgct gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa
     3301 tcgccagcgc ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca
     3361 gcgtgaacga cacggcgtag aaaaagacga tgataaacaa aatgccaaac gcgatattca
     3421 ttgcccgcaa tgctttatgc cgcgctactt caatcgattt ttcccgcgag cgataagaga
     3481 tcaccatcgg gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa
     3541 taatagcgtt tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc
     3601 acattcccac catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc
     3661 cagtcgaaat tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa
     3721 tcagtaccag accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat
     3781 gcaggtagga agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca
     3841 gcattacgaa atagagtgca cctaacagga tgccccagtt tttacctaaa taaccgctaa
     3901 tgacgctcgg gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct
     3961 gaaacagata cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca
     4021 tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca
     4081 taatcaccca gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac
     4141 tcattccagc acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa
     4201 tttccataat ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca
     4261 aggcgatatg cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc
     4321 gaggtcagag aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt
     4381 ctactaaatt aatcgcaaga gaatattcac tctctatttg cggcagatca caaaaaaaag
     4441 gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta
     4501 atggccagtc cgccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca
     4561 tacatcgtct cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc
     4621 atccgcgcgg cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt
     4681 tgtacctgtc cggcaaccgg atcgcaggtc agtccaaggt tatgctccat cgcgatttcc
     4741 gccgcattgc atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc
     4801 attgaacagg ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg
     4861 ttcattttat acagcgcgcc aatagccccg gctgcaagaa aatagcgggc aattgaccgc
     4921 tcgtttaccg gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac
     4981 gcaccgttag tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc
     5041 agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgctg
     5101 gaaaccagct gacgacgcag cgctacggca cggcgcggca cattgagcgg accaggcagc
     5161 acgccttcag tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca
     5221 aaaccggcgt caatttccgc tttactgcgc agcgctagct cgttgtgcat catcaggcca
     5281 gaaatagaca ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg
     5341 taaggtacgg gtgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac
     5401 ccgccgccga cagagtaata ggttttactt aatagctctt cctgtgcctt ccaggcagtg
     5461 atccgcattc cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct
     5521 acaggaaaat caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact
     5581 aactctataa atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt
     5641 cccatgatga tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac
     5701 agatcgacca caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata
     5761 aaactttttc cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt
     5821 ttgaaaatat cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg
     5881 agcggaacga ccttacgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag
     5941 cttcaatttc cagcttcaca tctttcggca aacgcgcgac ctgtacacag ctccgggtcg
     6001 ggtaggtcgc ctgatgctca tcgaagaatt gcttatagac ttcgttgatg gtggcaaaat
     6061 cattcagatc ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag
     6121 caaccacgat cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg
     6181 ggatctcacc ggtctgtggg caaaccggaa tttgaccgga ggtgaagacc atgctgccta
     6241 aatcaacgcc ttgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct
     6301 ttttcataca tcctccggcg tcagagcgcc tgggtaaagg tacgtgaaat aacatcctgc
     6361 tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg
     6421 ttaggatatt tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg
     6481 acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg
     6541 ccgacaaggt tggttttacg tactggatct tctttgccca gcgccgcagg gacgattgag
     6601 aaggtgtacg agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat
     6661 gaggccacgg cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt
     6721 gttccggcgc gacgaccgtc cggcgtgtta ccggttttct ggccgtacac cacgttagaa
     6781 gtgatagtca gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt
     6841 ttcttcataa aacgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg
     6901 ccgtactgcg gatattcacc gtcgatttca aagtccaccg ccaggccgtt tttgtcacgg
     6961 attggtttca cgcgggcata tttgatcgca gacagggagt ccgttgccac cgacaggccc
     7021 gcgatgccgc acgccatggt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct
     7081 tcgtagctgt acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc
     7141 gccagccagt ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacatcg
     7201 tccatcaacg gtgccgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg
     7261 ttaattgcgt acagcagtgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc
     7321 ttaccaatca ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca
     7381 gtacgcatca gatcgtcgtt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg
     7441 gcatattttt tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc
     7501 ggtgcaggcc ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc
     7561 gtacgaccgt ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag
     7621 aacagcgaat caaattccgg cgtacgcaag aagcgcacca tacggatctt catgatgaag
     7681 tgatcgatca gttcctgtgc ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca
     7741 atgtagatat ccaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc gttctgtgat
     7801 ttcactgccg ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc
     7861 gggcgagaga tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga
     7921 cgatgctctg ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc
     7981 agacgagact ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca
     8041 tacagcgcta cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa
     8101 ccggtcagca cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg
     8161 ccctggttat gggttttacg cagatcggta aacagatatt caaattcact gtccatttca
     8221 cgaccatagg cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtaacgca
     8281 cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat
     8341 cccgcatcat gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg
     8401 gttgcgtttt caatacgaat gccttccatc accttttccc acaattccgt ggtggctggc
     8461 gtcgcttcag cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa
     8521 tcgcggacat taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg
     8581 tacagcttat cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt
     8641 caactaaatt atgcaaattc tgcgggtgcg ttaactttgc ctaaatgaat ggcatccaaa
     8701 gcaatcattt tttcttcgtt ggtcggaatt acggcgcaaa tgacacgcgc attttcactg
     8761 gaaacaattc gctcgccaaa agagttagat cgattattca tttctgtatc aatctctacg
     8821 cctaatacag ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt
     8881 ccgccggtga atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga
     8941 cgggcaattc ggtgaacaaa ggttttaatt gccagctgcg cgcgttcgtg accttcatgc
     9001 caggcttttt ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac
     9061 tctttattca ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag
     9121 gacatcgcac caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc
     9181 gtcattccca ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca
     9241 ttgccaagat gcgccacaac caggccggag tcatcttccg ccagattcag caaagaatgt
     9301 gcgcgcaggg aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac
     9361 tcttcataat atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg
     9421 aaactggtat caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca
     9481 ataccactta aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta
     9541 tcaatgactt catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg
     9601 cggtggccaa ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt
     9661 gccttcaatg caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg
     9721 gataagaatg cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag
     9781 tcgctggcat cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca
     9841 accggaaatt cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttatatac
     9901 gatgttcagg atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg
     9961 gtatttcgcc agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga
    10021 tgcgataatc ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc
    10081 aacaacccag gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga
    10141 tactttagtt ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag
    10201 cgttcccaga tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc
    10261 cgcatattcc agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt
    10321 agccagataa gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga
    10381 cagcgtaaac aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc
    10441 acgagaaatg atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata
    10501 ctcttcacgt ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac
    10561 catgatggaa atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag
    10621 cgaaccgagg tcaacctggt cgataactgc agagttccag taagggatca gcgacaaaga
    10681 aatcagcacc aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag
    10741 atccttacca aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc
    10801 gcgattcagc ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat
    10861 agtaacgcca taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac
    10921 cacgccgcca gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt
    10981 agagccagaa agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc
    11041 caataccaac atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa
    11101 cagcaccccg gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt
    11161 acgccaggac gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta
    11221 tcctcaacga attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc
    11281 gatattgccg ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt
    11341 accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg
    11401 ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc
    11461 gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc
    11521 cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat
    11581 gccgtgaacg ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat
    11641 tgccaccgca ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac
    11701 atcatagaga tcttccataa tttccaggcc aatcgttccc tgaccagcaa tcactttcgg
    11761 atcatcgtaa ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc
    11821 gatagtgtcg ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc
    11881 cgctactttg gatttcggcg cgccttttgg catcaccact ttaccgtcga tacccagcat
    11941 cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt
    12001 gcgtttttcc gcatcagtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga
    12061 acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa
    12121 atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc
    12181 aataatgtca tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg
    12241 cctgaattaa atatgtaggt aaccgtaacc gacacctgca agacagacag gtggattatt
    12301 tatgtttaaa taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca
    12361 ttataagatg aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga
    12421 taatttttcg accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata
    12481 gtaataaatt gattagaacc aaaaggagac gtcatatcac aaggaattac agttaagaaa
    12541 tcagcattga gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca
    12601 atactgatgc catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt
    12661 tgtggcaaca cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt
    12721 cgggacttac tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc
    12781 tgaagcttca tttctgcgct taacgtacca atcgcaaaat caagtcgacc gtcgcggatt
    12841 gccggtaaga acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc
    12901 tctttgaact tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca
    12961 aatgagactt ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc
    13021 atttcacggg taatggattc ggaacgagag agtaacaatt gaccggcagg tgttaatgtt
    13081 actccggtat ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg
    13141 attttactga cggccggttg agttaaccct aattcttttg cagccgagcc gatagaacca
    13201 cttctaatga cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc
    13261 ataacgacct acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca
    13321 ggatatgtgc gaccactcac aaattaactt tcaatacttt ccagggtatc gtcatgtcaa
    13381 ttaaatacct ttaaatatca ataaattaaa gtataaaaat cagcataaac cctgattttt
    13441 gtcaaaaaaa atgacg
//
LOCUS       DAZKFZ010000116        13378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000116 DAZKFZ010000000
VERSION     DAZKFZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(119..823)
                     /locus_tag="ACHOQW_003049"
     CDS             complement(119..823)
                     /locus_tag="ACHOQW_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9574092.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(959..1411)
                     /gene="moaE"
                     /locus_tag="ACHOQW_003050"
     CDS             complement(959..1411)
                     /gene="moaE"
                     /locus_tag="ACHOQW_003050"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP9574093.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1413..1658)
                     /gene="moaD"
                     /locus_tag="ACHOQW_003051"
     CDS             complement(1413..1658)
                     /gene="moaD"
                     /locus_tag="ACHOQW_003051"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP9574094.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1651..2136)
                     /gene="moaC"
                     /locus_tag="ACHOQW_003052"
     CDS             complement(1651..2136)
                     /gene="moaC"
                     /locus_tag="ACHOQW_003052"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP9574095.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEVD
                     D"
     gene            complement(2139..2651)
                     /gene="moaB"
                     /locus_tag="ACHOQW_003053"
     CDS             complement(2139..2651)
                     /gene="moaB"
                     /locus_tag="ACHOQW_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP9574096.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2673..3662)
                     /gene="moaA"
                     /locus_tag="ACHOQW_003054"
     CDS             complement(2673..3662)
                     /gene="moaA"
                     /locus_tag="ACHOQW_003054"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP9574097.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3650..3794)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4059..4967
                     /gene="yvcK"
                     /locus_tag="ACHOQW_003055"
     CDS             4059..4967
                     /gene="yvcK"
                     /locus_tag="ACHOQW_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9574098.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5158..7179)
                     /gene="uvrB"
                     /locus_tag="ACHOQW_003056"
     CDS             complement(5158..7179)
                     /gene="uvrB"
                     /locus_tag="ACHOQW_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9574099.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7758..8435)
                     /gene="bioD"
                     /locus_tag="ACHOQW_003057"
     CDS             complement(7758..8435)
                     /gene="bioD"
                     /locus_tag="ACHOQW_003057"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9574100.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8428..9183)
                     /gene="bioC"
                     /locus_tag="ACHOQW_003058"
     CDS             complement(8428..9183)
                     /gene="bioC"
                     /locus_tag="ACHOQW_003058"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP9574101.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(9170..10324)
                     /gene="bioF"
                     /locus_tag="ACHOQW_003059"
     CDS             complement(9170..10324)
                     /gene="bioF"
                     /locus_tag="ACHOQW_003059"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP9574102.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISAFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10321..11361)
                     /gene="bioB"
                     /locus_tag="ACHOQW_003060"
     CDS             complement(10321..11361)
                     /gene="bioB"
                     /locus_tag="ACHOQW_003060"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP9574103.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11448..12737
                     /gene="bioA"
                     /locus_tag="ACHOQW_003061"
     CDS             11448..12737
                     /gene="bioA"
                     /locus_tag="ACHOQW_003061"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP9574104.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12796..13272
                     /locus_tag="ACHOQW_003062"
     CDS             12796..13272
                     /locus_tag="ACHOQW_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP9574105.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         3084 a         3637 c         3439 g         3218 t
ORIGIN      
        1 cggaaaactc ttccgctcgg ccataaggcg tattttatac ctggtacttg ttaagtgtcg
       61 tttttagtaa gaaaaaaaat cccgacacat ggccgggata ttgatggtga aaaaagaatt
      121 aacgacgatt gccaaaaatt cgcagcaaca tcaggaacag gttgatgaag tccagataca
      181 aggttaatgc gcaaagaatg gaatatttgc gcaggttcga cgcgtcgcgg gtatctatct
      241 gctcacccat atttttcagt ttctgggtgt catacgccgt caatccgaca aagacaatca
      301 cgccgatgta ggtgactgcc cacatcaacg cttcgctttt cagccagaag ttgaccagcg
      361 atgccaacac aatgccgatt aacgccataa acagcatatt gccaaagcca cttaaatcgc
      421 gcttcgtggt gtaaccgtac aggctcattg cgccgaacat cccggcagta acgacgaagg
      481 tactggcgat agaagcagcg gtataaacaa tgaaaatact ggaaagcgta agacccgtca
      541 gcgccgaata aagcataaag agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg
      601 ctgataacac aataaccaat gctaattgcg cgatgatcag accgattaaa aagacacggt
      661 tagtgaacaa cagctccatc acggccgcgg aattagccgc ataccaggca acaaatgcgg
      721 tcagcaataa gccaacggtc atccagccat agacttgcgc catataagtc tgcaagccag
      781 cccggggttg tacaattgaa tcagaacgtg ggaatctgtc catgacgatc tcctgaagat
      841 ataaggaata tcttaaggat actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac
      901 gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc ttatccggct aacaaaaact
      961 accagcgttt tgccgcctgc tgatcgctct cccgagcttc gacccagcgg tcgccctccg
     1021 gcgtggcttc gcgcttccag aacggtgcgc gggttttgag ataatccata ataaactgcc
     1081 cggcttcaaa cgcactgctg cgatgcgcac tggtgacacc gataaaaacg atttcatcgc
     1141 ccggccataa ttccccgatg cggtgaatca ccgtgacgcg ccccagcggc cagcggttac
     1201 gcgcttcatc gacaatttct gccagtgctt tttcagtcat ccccggatag tgttcgaggg
     1261 ttaatgcttt gacgctgtcg cccaggttat ggttgcgcac cttaccggta aaggtgacta
     1321 ccgcaccgtc ttcgtcacgc tccgccagcc acgggtactc ttctcctacg ctgaacggct
     1381 gcggaccaac aacaattttg gtttctgcca tcttaacctc cggttaccgg cgggaagaaa
     1441 gctacttcgt cgccgtcagt cagcggatgg tcaaaactca ccagcgtctg gttgacggca
     1501 gccagtaatt tgccatcttc cagcgccagc gcccagcgat cgctctgcgc agccaggtgc
     1561 tggcgtaacg cttcaacggt tgggaaatcc gcagccactt cggttgcatc tgttcctacc
     1621 aactcgcgca cctgggcgaa aaaaagaact ttaatcatcc acttccacct taaagtcacc
     1681 cgacttgccg ccgctcttcg ccagcaaacg taccggacca atcaccatat ctttttgcac
     1741 cgctttgcac atgtcataaa tggtcagcgc cgccacggag gccgcggtta acgcttccat
     1801 ttcgacaccg gttttcccgg tcaggcggca taaggtttct atacgcaccc gattgtgctc
     1861 cggctcggcc tgtaaattga cttcaacttt gctgagcatc agcggatgac acagcgggat
     1921 cagatcccag gtgcgttttg ccgcctgaat accggcaata cgcgcagtgg caaatacgtc
     1981 gcctttgtgg tggcgaccat caataatcat cgccagcgtc tcgctgcgca tggtgacaaa
     2041 ggcttccgcc cgcgcttcac gcacggtttc cgctttggcg gagacatcca ccatgtgcgc
     2101 ttcgccagcg gcgttgatat gggtcagttg cgacatactt atttcttcaa atgtggatgg
     2161 aaattacacg gacgcgtacg ggcatccagc tgcggcgcga tgatattttc ccatgcggta
     2221 cggcacgctt tggtcgaacc cggcatggcg aaaatcagcg ttttgttagc gacgcccgct
     2281 accgcacgag attgcaacgt ggaagtgcca atctcttcaa acgacaacat acggaacact
     2341 tcaccaaaac cttcaacttc acggtcgaac aacggcagca atgcttcggg agcctgatca
     2401 ccttccgtca ggccagtccc ccccgtaatc aacaccactt gtacatcgtc gctggcgatc
     2461 cacgcagata cctgagcgcg aatagcgtag cggttttctt tcacaatggc tttatcgaca
     2521 acgtgatggc ccgcttcttg cgccgaatcg cgcaaatagt gaccggaggt atcgtcttct
     2581 tcaccgcgac gattagaaac cgtaagaata gcaatacggg tcgggataaa ttcagtgctt
     2641 acctgactca tctgatctct ccttttgacg ttttagccgc caatgtacga taagttttgc
     2701 gtaataccgg tgttgttttg atgcaggaaa tgggtctgtt tcttctcccg cagcgccgct
     2761 gaaatacgcg cttccagcgc ctgttgctgg gtatcgtctt ccagcagatc gcgcaggtta
     2821 acgccgcctt caccaaacag gcagagatgg agtttaccaa tggaggaaac gcgcagacgg
     2881 ttgcaagtgg cgcagaagtc tttttcatac ggcatgataa ggccaatctc tccggcgtaa
     2941 tccggatgac aaaagacttg cgcgggaccg tcgctgcgtt gacgtaattg gtggatccag
     3001 ccgcgacgca gtagctcgtc acgcagaacc tgaccagaga tgtgatgctt acggaaaagc
     3061 tcgctgccct cgcccgtttc catcagttcg atgaaacgca gctggatagg gcgatgctgg
     3121 atccagttca gaaaggtgtc gagctggtga tgattcacat cacgcatcag cacggtattg
     3181 actttgacct tctcaaaacc ggcctcaaat gcagcatcga tccctgccat cacctgattg
     3241 aatttatcct ggccggtaat ggcatgaaac tggcgggcgt ccagactgtc gacgctgacg
     3301 ttaatgccag taagtcccgc atcgcgccag ttcgccacat cgcgttccag acggtaacca
     3361 ttggtggtga ccgctatctg gcgaatagcg tcgttttctc gcacagcggc gatgatatcg
     3421 gtaaagtcgc ggcgtaaaga cggctcaccg cctgtcaggc gcactttttc ggtgcccaga
     3481 ctggcgaagg cgcgcgtaac ccggcgaatt tcatcgacgg tcagaaagcc tttattggtg
     3541 acgccgctcg gtttgtagcc atccggcagg cagtaggtgc aacgaaagtt acacacatcg
     3601 gtaatcgaca ggcgcaagta gtaaaactta cgcgcaaatg catcagtcag ttgtgaagcc
     3661 atgtacacct ttccagatac gggaggcgaa gtcatttctt ccttcgccct ggtggcaatc
     3721 tttacgagca ttgtcacggc caaagcacca tatcagttga cccaggtgca gaggctagag
     3781 tgtttagtgg ttaagccgat actagcgtgt aaatgagatt tttaccatcc acactttcgc
     3841 tatgtaatca tgtatatagc gtcctgatcg tgctatttcg ctacagaata agggaaaatg
     3901 gcggttttac tttgatatac atcatcaggg aggcatagcg tgcatcgtcc tttagaggta
     3961 gaagaaaata gtttgtatcc tcaatattgg caggttaatt gctgtttccc cgcaatttgc
     4021 gctactgtag cgcggataat ctgactccag gaaactatat gcgcaatcgt acgctggctg
     4081 atcttgatcg tgtcgttgct ctcggcggag ggcatggact gggacgcgtt ctctcatcac
     4141 tttcgtcttt gggttctcgt ttaacgggta tcgtcaccac cacggataat ggtggctcga
     4201 cggggcgtat tcgccgttca gaaggcggca ttgcctgggg cgatatgcgc aactgcctta
     4261 accagctgat aactgaaccc agcgtcgctt cagcaatgtt tgaatatcgt ttcggcggta
     4321 atggcgaact ttccgggcac aaccttggaa acctgatgtt gaaggcactg gatcacctta
     4381 gcgtgcgacc tcttgaagcg atcaatctga ttcgaaatct gctgaaagtg gacgcacatt
     4441 tgattccgat gtcggagcat cctgttgatc tgatggcgat tgacgatcag gggcatgaag
     4501 tttacggcga ggtcaatatc gaccagttaa ctacgccgat tcaagagtta ttgttaacgc
     4561 ctaatgtacc cgcaacgcgt gaggcggttc acgctatcaa tgaagcggat ctcatcatta
     4621 ttgggcctgg cagtttttac accagcctga tgccaattct gctgctgaag gaaatcgcgc
     4681 aggcattacg ccgcacgcca gcgccgatgg tctatatcgg caatctgggg cgtgagttga
     4741 gtttacctgc ggccaatttg aagctggaaa gcaagctggc aattatggag cagtatgttg
     4801 gtaaaaaggt cattgatgcg gtcatcgtcg ggccaaaagt ggatgtctcg gcggtgaaag
     4861 agcggattgt gattcaggag gtgctggagg ccagcgatat tccgtatcgt catgaccgcc
     4921 agttgttaca tagcgcactg gaaaaggcgt tacaggcttt aggttaacgg aaatccgaag
     4981 gaaaattccg gcttcctaat gaagacaaag tgcgcgttgt ttatgacgga tgcggcgtga
     5041 acgccttatc cggcctacaa accgcgctaa ttcaatgtat tgcagaatca tgtaggcctg
     5101 ataagcgtag cacatcaggc aattttgctt ttgtcatctg tcttcttcgt tatcctgtca
     5161 cgaagccgcg ataaacaatt cacgcagctg atgcaactgg tcacgaattt gtgccgcttc
     5221 ttcgaactcc agattttgcg cgtgttgcat cattaaccct tccagctcat ggattttctg
     5281 ttgcaacgct ttaggcgaca tatccatcgg cacattatcc ggctcaacaa tcggtcgcga
     5341 ttttcctctg cccttcgctt tggttttagc aatgttctgc cccaacgcca ggatatcgac
     5401 cactttcttg ttcaagcctt gcggcgtaat gccgtgttcc tcgttgtact tctgctgttt
     5461 ctcgcggcga cgttcggttt cgccaatcgc tttcgccatt gacggggtga tcttatcgcc
     5521 gtagagaatc gctttaccgt taacgttacg cgccgcacga ccaatagtct ggatcaacga
     5581 acgttcagaa cgcaggaagc cttctttgtc ggcgtcgagg atcgccacca gcgaaacctc
     5641 cggcatatcc agaccttcgc gcagtaagtt gatccccacc agcacgtcga actcgcccag
     5701 acgcaagtcg cggataatct ccatacgttc gacggtgtcg atatctgagt gaagataacg
     5761 cacgcgctca ccgtgttctt cgagatattc ggtaagatct tccgccattc gcttggtcag
     5821 tgtggtgacc agtacgcgtt cgttaattgc cgctcgctga cgaatctccg aaagaagatc
     5881 atcaacctgt gtcgccactg gccgcacttc gataatcgga tcaagcaatc cggttggacg
     5941 caccacctga tccaccacat cgccgccgga tttttccagc tcataattac ctggcgtcgc
     6001 cgaaacatag atggtttgcg gcgctaatgc ttcgaactct tcaaatttca gcggacggtt
     6061 atccagcgct gatggcaggc ggaagccgta ctccaccagt gtctctttac gcgcccggtc
     6121 accgcgatac atgccgccaa tttgtggaat ggtgacgtga gattcatcga ccaccagcag
     6181 cccatcggca ggcaggtaat caaacagcgt cggcggtggc tcacccggtc cacgaccgga
     6241 gaggaagcgc gagtagtttt caatccccga acagtagccc agctcgttca tcatctccag
     6301 atcaaactgg gtacgctggg tcagccgctg ctcttccagc agtttgttgt tttccaatag
     6361 cactttgcgt ctggcggcca gctcttcttt gatctcctcc atcgcctgta cgatgcgctc
     6421 gcgcggtgtg acgtagtgcg ttttcgggta gatggtaaaa cgtggaatgg tggaaacaat
     6481 ctgcccggtt agcgggtcaa ataacgacaa tcgttccact tcctcgtcaa acagttccac
     6541 gcgaagtgca atgtcatccg attctgccgg gaagatatca atcacctcgc cacgaacacg
     6601 gaaagtacca cgctggaatg cttgatcatt acgagcgtat tgcagctccg ccagtcggcg
     6661 cagaatcgcg cgctgatcga taatcatacc gaccgtgaga tggagcatca tcttgagata
     6721 taaatcagga tcgcccagac cataaatcgc ggaaacagac gcgaccacaa caacatcacg
     6781 ccgctccagc atcgctttgg tggcggacaa acgcatctgt tcgatatgtt cgtttaccga
     6841 ggcatctttc tcaatgaagg tgtcggaact cggtacatag gcttctggct gatagtagtc
     6901 gtagtaggag acgaaatatt ccaccgcgtt ttccgggaag aactctttca tttcgccata
     6961 cagctgggcc gccagcgttt tgttgggcgc aagtaccatg gtagggcgct gaaggtcagc
     7021 aatgacattg gcaatggtga aggttttccc ggaaccagtc acaccaagta acgtctggtg
     7081 cgccaggcca tcttccagcc cctcttcgag acgtcgaatc gcctctggct gatcgccaga
     7141 aggtttaaaa gcggaattca gtttgaacgg tttactcatg agtcgctacc tgaaggagtt
     7201 gggcgggcag gtatgtaatt ttactcgtcg tacttaatta tgccaacaaa ttatactgga
     7261 taaaaaaaca gttcatcacc ataatatttc tgttacagcg taaactccgc tgtcaatcat
     7321 gagtaaaatt tactctgtgg cgagataaaa ctcggacctt accgggttat ccccaaagca
     7381 acggcttttt taacatttgt caagatgagt gatgtcagtt ttatggcaag agatgcctgt
     7441 tcagtgactg ccattgcaat tatctaacca gttaaaaaat aaaagatatt tctttgagcc
     7501 gtctttaacg ccaattcgcg tgcaagccgc gtattctctc gcttgcctcg tgttttctaa
     7561 ctctaataca catggttatc cacaggaata gtggataact gtctgcagcc cctatccccc
     7621 gccgcctggg caattcccac attcgccgca tgatgcggaa gtaaaatttt tttcgtgatt
     7681 attttagaat ttaattggtt aaattgcagt caatcgaaga cgcgatctcg ctcgcaattt
     7741 aaccaaatac aggatggcta caacaaggca aggtttatgt actttccggt tgccgcattt
     7801 tctggatttt ctgcaagcca ggggatctct cccagcagcg gcgcgggaat catgcgggtg
     7861 agcgtggtca tatattcagc gtgacgtttt cccggaggcg tgacatcgtt cgccacccaa
     7921 cccgccagag tcagcccggc gtgttgtatc gcctgtgccg tcaacatcgc gtgattaata
     7981 cagccaagtt tcacgccaac caccagtatt accggcagtt gttcctgtgt tacccaatcc
     8041 gcaaaagtga aagtgtcaga aagcggcgta aaccagccgc cagcgccttc cactaacacc
     8101 cagtcagcct gttgttcaag cgcgcgtaat ccggagctca ttaccgatga ttctatcgac
     8161 ctgccctctt gcgcgctgat gatgtgcggt gaggtgggtt ctgcgaaggt gtaaggattt
     8221 accgttgcgt aatccagctg caggctgctg ttgcgctgta acgccagcgc gtcactatta
     8281 cgtaaaccct ccggggtctt ttcgctgcca gaggcgaccg gtttataacc tgccgtccga
     8341 tagcctgctg cctttgcggc ttgtaaaagt gcacaactgg cgacagtttt ccccacttcg
     8401 gtatccgttc cggtgacaaa ataacgttta ctcacgaaca atcactccca aaaaaagatg
     8461 atacgtcaga ggatatcgcc cctgttgttg cggccaggcc agttgcaatc gctgcaactg
     8521 tgaacgcgtt aatattcgct ggtcgcgccc ttcatgaaga tgcgtggcac cgatgccttt
     8581 cagcgaacgc atggcactga gcgcatcatc aaaccatagt gtgatgggct gaatacgatg
     8641 ttgataatgc acgccgttca gcgactgttc gatttcatct ggcggtaaaa agcgattagc
     8701 atgcgggcgc tcgtccaccg cctgccacgc ctgatgcagt tcgggtaacg atccctgcac
     8761 cagcgtggta aacgcgacca cgcctccggg gcgcaccacc cgatacagct cgcggagtgc
     8821 cgttgataaa ttaccgcacc actgcaccgc gagattgctc catgcaagat cgaacgtcgc
     8881 agtcgctaac ggcagggatt cgatatctcc cgccagataa tggtctgcag catctttctg
     8941 gcgggcttgc gcaagcattg gcggcgagag atctaacgcc gtcacctgcg cgtgacgctc
     9001 tcgccagtgg cggctcatcc agccaggccc acaccccgcg tccagtacgt gggggtattt
     9061 acgctgtggg agcattgcca gtaagacatc agcactctgg cgctgtaact ctgcgtgttg
     9121 ctcatagtgc gcggctgccc gaccaaatgc cgctgcaatg gcttgtttat taaccgttgc
     9181 catgcagcac ctccagcaga cggtcgatat cctgcatttc atgcgcggcg gttagcgtta
     9241 agcgcagtcg cgcagtacca gagggtacgg ttggcgggcg aatcgccgtg acccagcagc
     9301 cttgctggcg cagtttttct gccagttgta acgcacggct gttatcaccg acgatcaatg
     9361 gctggatggc gctgcatgaa tcagcaagcg taaacggcaa atcctgtact ccggcacgaa
     9421 aacgcgtaat gagtgccgcc agtttttcgc gccgtgcatc accctcatca ctgcgaatga
     9481 ccgccagtga cgcacgtaat gcctgcgcct gagcgggcgg catactggtg ctgtagataa
     9541 gatggcgggc gaattgcagc agataatccg ccaccgtact ggagcaaagc accgcagccc
     9601 cgctgacgcc aaatcctttg ccaaaagtca ctaccagcaa ttctggtttt accttttgca
     9661 accagcaggt gccgcgcccc tgctccccga taacgcccgt gccgtgggca tcatcgacca
     9721 tcaaccagcc attgtgctgt tgcgttacct gctggatttc cgccagtggc gcactatcgc
     9781 cgtccatgct gaacacgcct tctgtcacca ccagttgctg ccccggacag ggggaagcaa
     9841 gcagtcgcgc cagatgagtg acatcgttat gaacaaaacg gcgaagctgc gacgggctta
     9901 aactggcagc ctccagcaat gaggcatggc taagccggtc agcgacaatg cggtcctctt
     9961 ttgccatcat cgcggcaatg accgcctgat tagcggcaaa agcagagata aatagcagtg
    10021 cccgcgaata gcccagccac tcggccagtt cttcttccag cgcctgatgc gcaacgctat
    10081 aaccgctgac gtgaccggaa ccgccgctac cgacgccaaa ttgctccgca ccctgcttcc
    10141 aggcacggat aatttgcgga tgatggctta aacctaaata atcgttactg gaaaagttca
    10201 gatactggcg atcgtccgcc accagccagc gtccggctcc ttgcgccact ggataacggc
    10261 gacgcagggc atcggcagca cgccgcgcat cgagcgccgc gttgattttc tcctgccagc
    10321 tcatagtgct gccgcgttgt aatattcgtc agtgtccggg gtcatcagtg cctgctccag
    10381 acgctgctgt tgttcgttat cccctgccag cacggcagtt tgctgcggat ttagccccag
    10441 tttgcgaaac agttgcaggt ctttatcttc ttccggattg ggcgtggtta gcagtttgca
    10501 accgtagaaa atcgagtttg cgcctgccat aaagcacatc gcctgagtct gttcgttcat
    10561 ctgctcgcgt ccggcagaaa ggcgcacgta agaggtcggc atcatgatcc gcgctacggc
    10621 aatggtgcga ataaaatcaa aggcatcgac atcatcgtta tctgccagtg gagtaccttt
    10681 caccttcacc agcatgttga ttggcacgct ttccggcggc gtcggcaggt ttgccagttg
    10741 cagtaataat ccggcgcgat cttttaccgt ttcgcctaaa cccacaatgc cacctgagca
    10801 gactttgatc ccggcatcgc gcactttttc cagcgtatca aggcgttcct gataagtacg
    10861 tgtggtgatg atattgccgt aaaactccgg cgaggtgtcg aggttatggt tgtagtaatc
    10921 cagcccggca ttcgcaaggc gctgcgcctg agattcactc aacgtaccca gcgtcataca
    10981 cgcctccagc cccatcgctt ttactccctg caccatttgt tccaggtatg acatatcgcg
    11041 ttcgtgggga ttcttccacg ccgcgcccat acagaagcgc gtcgatcctg ccgctttcgc
    11101 tttgcgcgcc gactccagca cctgttcaac ttccatcaac cgctcggctt ccagcccggt
    11161 tttgtagcgc gagctttgcg ggcagtattt gcaatcttcc ggacaagctc cggtcttaat
    11221 cgacagcaag gtgctgacct gcacctgacg aggatcgaaa tgctggcgat gcacctgctg
    11281 cgcttcaaac agcagatcca gcaacggttt ttcaaataat tctgtgactt gcgacaatgt
    11341 ccagcgtggg cggtgagcca tggggcttct ccaaaacgtg ttttttgttg ttaattcggt
    11401 gtagacttgt aaacctaaat cttttcaatt tggtttacaa gtcgattatg acaacggacg
    11461 atcttgcctt tgaccaacgc catatctggc acccatacac atccatgacc tccccgctac
    11521 cggtttatcc ggtggcgagc gccgaaggtt gcgagctaat tttgtctgac ggcagacgcc
    11581 tggttgacgg tatgtcgtcc tggtgggcgg cgatccacgg ttacaatcac ccgcagctta
    11641 atgcggcgat gaagtcgcaa attgatgcca tgtcgcatgt gatgtttggc ggtatcaccc
    11701 atgcgccagc cattgagctg tgccgcaaac tggtggcaat gacgccgcaa ccgctggaat
    11761 gcgtttttct cgcggactcc ggttccgtag cggtggaagt ggcgatgaaa atggcgttgc
    11821 agtactggca agccaaaggc gaagcgcgcc agcattttct gaccttccgc aatggttatc
    11881 atggcgatac ctttggcgcg atgtcggtgt gcgatccgga caactcaatg cacagcctgt
    11941 ggaaaggcta tctgccagaa aacttgtttg ctcccgcccc gcaaagccgc atggatggtg
    12001 aatgggatga gcgcgatatg gtgggctttg cccgcctgat ggcggcgcat cgtcatgaaa
    12061 tcgcagcggt gatcattgaa ccgattgtcc agggcgcagg cgggatgcgc atgtaccatc
    12121 cggaatggtt aaaacgaatc cgcaaaatgt gcgatcgcga aggtatcttg ctgattgccg
    12181 acgagatcgc caccggattt ggtcgtaccg gcaaactgtt tgcctgtgaa catgcagaaa
    12241 tcgcgccgga cattttgtgc ctcggtaaag ccttaaccgg cggcacaatg accctttccg
    12301 ccacactcac cacgcgcgag gttgcagaaa ccatcagtaa cggcgaagcc ggctgcttta
    12361 tgcacgggcc aacttttatg ggcaatccgc tggcctgtgc ggctgcaaac gccagcctgg
    12421 cgattctcga atctggcgac tggcagcaac aggtggcggc tattgaagta cagctgcgcg
    12481 agcaacttgc ccccgcccgt gatgccgaaa tggttgccga tgtgcgcgtg ctgggggcca
    12541 ttggcgtagt cgaaacaact cgtccggtga atatggcggc attgcaaaaa ttctttgtcg
    12601 aacagggtgt ctggatccgg ccttttggca aactgattta cctgatgccg ccctatatta
    12661 ttctcccgca acagttgcag cgtctgaccg cagcggttaa ccgcgcggta caggatgaaa
    12721 catttttttg ccaataacgg gaagtcagcg tgagagtttc tggctacact ttctgcaaac
    12781 tagaaaggag ggtttatgaa actcatcagt aacgatctgc gcgatggcga taagttgccg
    12841 catcgtcatg tctttaacgg catgggttac gatggcgata atatttcacc gcatctggcg
    12901 tgggatgatg ttcctgcggg aacgaaaagt tttgttgtca cctgctacga cccggatgcg
    12961 ccaaccggct ccggctggtg gcactgggta gttgttaatt tacccgctga tacccgcgta
    13021 ttaccgcaag ggtttggctc tggtctggta gcaatgccag acggcgtttt gcagacgcgt
    13081 accgattttg gtaaaaccgg atacgatggc gcagcgccgc cgaaaggcga aacccatcgc
    13141 tacattttta ccgttcacgc gctggatgta gagcgtattg atgtcgatga aggagccagc
    13201 ggcgcgatgg tcgggtttaa cgttcatttc cactctctgg caagcgcatc gattaccgcg
    13261 atgtttagtt aatcactctg ccagatggcg caatgctatc tggtatcact taaaggtatt
    13321 aaaaacaact ttttgtcttt ttaccttccc gtttcgctca agttagtata aaaaagct
//
LOCUS       DAZKFZ010000117        13278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000117 DAZKFZ010000000
VERSION     DAZKFZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(57..1415)
                     /gene="plaP"
                     /locus_tag="ACHOQW_003063"
     CDS             complement(57..1415)
                     /gene="plaP"
                     /locus_tag="ACHOQW_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP9574106.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1405..1467)
                     /gene="yoeI"
                     /locus_tag="ACHOQW_003064"
     CDS             complement(1405..1467)
                     /gene="yoeI"
                     /locus_tag="ACHOQW_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP9574107.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1682..2610)
                     /gene="yeeY"
                     /locus_tag="ACHOQW_003065"
                     /pseudo
     CDS             complement(1682..2610)
                     /gene="yeeY"
                     /locus_tag="ACHOQW_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(2656..3480)
                     /gene="yeeZ"
                     /locus_tag="ACHOQW_003066"
     CDS             complement(2656..3480)
                     /gene="yeeZ"
                     /locus_tag="ACHOQW_003066"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9574108.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            3906..3956
                     /gene="hisL"
                     /locus_tag="ACHOQW_003067"
     CDS             3906..3956
                     /gene="hisL"
                     /locus_tag="ACHOQW_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP9574109.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    3932..4055
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4102..5001
                     /gene="hisG"
                     /locus_tag="ACHOQW_003068"
     CDS             4102..5001
                     /gene="hisG"
                     /locus_tag="ACHOQW_003068"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP9574110.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5007..6311
                     /gene="hisD"
                     /locus_tag="ACHOQW_003069"
     CDS             5007..6311
                     /gene="hisD"
                     /locus_tag="ACHOQW_003069"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP9574111.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6308..7378
                     /gene="hisC"
                     /locus_tag="ACHOQW_003070"
     CDS             6308..7378
                     /gene="hisC"
                     /locus_tag="ACHOQW_003070"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP9574112.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7378..8445
                     /gene="hisB"
                     /locus_tag="ACHOQW_003071"
     CDS             7378..8445
                     /gene="hisB"
                     /locus_tag="ACHOQW_003071"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP9574113.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8445..9035
                     /gene="hisH"
                     /locus_tag="ACHOQW_003072"
     CDS             8445..9035
                     /gene="hisH"
                     /locus_tag="ACHOQW_003072"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP9574114.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9035..9772
                     /gene="hisA"
                     /locus_tag="ACHOQW_003073"
     CDS             9035..9772
                     /gene="hisA"
                     /locus_tag="ACHOQW_003073"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP9574115.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            9754..10530
                     /gene="hisF"
                     /locus_tag="ACHOQW_003074"
     CDS             9754..10530
                     /gene="hisF"
                     /locus_tag="ACHOQW_003074"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP9574116.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10524..11135
                     /gene="hisIE"
                     /locus_tag="ACHOQW_003075"
     CDS             10524..11135
                     /gene="hisIE"
                     /locus_tag="ACHOQW_003075"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP9574117.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11232..12209)
                     /gene="wzzB"
                     /locus_tag="ACHOQW_003076"
     CDS             complement(11232..12209)
                     /gene="wzzB"
                     /locus_tag="ACHOQW_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP9574118.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12355..>13278)
                     /locus_tag="ACHOQW_003077"
     CDS             complement(12355..>13278)
                     /locus_tag="ACHOQW_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFP9574119.1"
                     /translation="PTDYDPKTNYFNTSSVESVIKDVVEINPYAVMVIKSTVPVGFTE
                     AMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNI
                     PTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLGLNSRQIIEGVCLDPRIGNH
                     YNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVV
                     GIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFK
                     QQADVIISNRMAEELRDVADKVYTRDLFGSD"
BASE COUNT         3352 a         3459 c         3394 g         3073 t
ORIGIN      
        1 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa tgatgattat
       61 gctacgtctt cgtactgcgg aaccgggtta cggaagctct tggtgacgca agccaggtaa
      121 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
      181 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
      241 ttcagcgtct tattacgctt ctcacggatc cagaactgcg agatgaccga caggttaacg
      301 aaggtgaacg ccaccaacgc accaaagttg atcagcgccg tagccattac caggtcgaag
      361 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
      421 ttcgggtgta cataaccgaa gaaacttttc gggaacacgc cgtcacgacc cattacgtac
      481 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
      541 aagatcagcg caccgacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
      601 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
      661 gcaaagatga agatcatgcc gccgatcagc gcggtcagga aaatcgcacg cgggatcacg
      721 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
      781 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaat
      841 ggacgagtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
      901 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gaagttcgct
      961 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     1021 atccacgatg ggatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     1081 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     1141 gggctaatgg atttctgggc gtaagtatat gcagagccag cagaaggata gcggcgaacc
     1201 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     1261 ggcacatgac catccgtgag gcctgaaacg ataccaaaag tatcaaacag cgtcatcggc
     1321 tgcatatagg caagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     1381 acgcgagagg tgtttggagt aacgttatgc gacatggtca ttctccttta cggtgataac
     1441 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     1501 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     1561 agcaaaaaat aaccgacgct gatgaaacgt cggtttttag tcattttttg acagcggcgc
     1621 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     1681 tttaaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     1741 ctaaaatttc atcccgccac cagccgctgg caagtcctgc ggtctgttca ttccagccaa
     1801 tccagacggg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     1861 cgagaaaacg ttgcgccaga taacggggta aatagccgca gccgaggccg ctaatttgca
     1921 gctccagctt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     1981 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     2041 tgatacgacg gtttaatggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     2101 agacttgttc cagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     2161 gcatcgcgcc aacaataata tccgctcgcc cctgagtcag ggcatcccat gaaccaccga
     2221 gtacgccatt gataaacttc aggcgcgtca cgttatgatg ttgataaaaa gcttcaatga
     2281 gtggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     2341 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     2401 gccccttctc taataacatt ttccggtacg ggtgaattta gcgcggtgac cgctacgatc
     2461 aagcaattga atattaaggt cgctttccag cttgtgaacg gtgtaactca gagcggacgg
     2521 cgtcttgtag agtttagccg acgccgccgc aaaactgcct tctttttcta aggcatcgag
     2581 gatcatcaac acatccagca gtggtttcat acacgccccc ttgcagtgca tgcggcgtcc
     2641 tgctggcgtg atcgtttact ccagcggcat taccagcgga tcgggatact ggtattcaaa
     2701 tcccagttca ttacaaatcc gactgccatc aataatctta cctttgccgc tgtccagact
     2761 atttctgaat tgcggaggtt ccagccccag taaacgggcc atctgcggat agaaaacatt
     2821 acgcgcaggg tgagctggcg cacatatatt atagatgtgt ccgcctttag gtgcctgtaa
     2881 caacagagtg atagcgccaa taacatcttc taaatggact aaattaacac catgttcacc
     2941 atcaggcgcg gtttttccgg caaagaagcg tccgggatga cgccccggtc cgaccaggcc
     3001 cgcaagacgc agaatatcga ccgaagtacc gggtaaattg tgcagccagt cttcgagttc
     3061 ttctaatact cgtccactgt tggtcaccgg attacgcggg gtggtttctt tcaccgtgcc
     3121 ttgcgcgtcg ccatagacag acgttgaact ggtaaaaata attcggggaa tacgatgggc
     3181 cagcgcgcta tccactaact cttgtaccgc ttgtaaatag aactcatcgc cggggccgct
     3241 acgacgggcc ggaagcgtaa tgaccagcgc atcggcatcc atcagggcat ccagatcgtc
     3301 agaatcgcaa actaactcag gctccatgcg aagcagatag ctatcaatgc cactcattcg
     3361 ggccgcttcg acaccatctt gtgtggtttt actcccggtg acttgccagc ctcgcgctga
     3421 aagtgacatc gccagcggca tgcctaacca ccctaacccg acaattgcga cctttttcat
     3481 ccgttatctc ctgatacacc ttagatctat aaggctacgc tagcgtatca aaactgacaa
     3541 ttcattctat gaatgaaatc gaaactgtta gcaaatatac cattgcctga tgcgctccgc
     3601 ttattagtcc tacaaaacat caatatattg aatttaggca acatttgtag accagataag
     3661 gcattcacgc cgcatccggc aggaacaaag cccactttag ttacagaaca caatgttctt
     3721 gagggtttta atgacatatt ttcacgatta cttttttaac ctagtcactc ttagccccct
     3781 cctttccccg ctcattcatt aaacaaatcc atcgccataa aatatataaa aaagcccttg
     3841 ctttctaacg tgaaagtggt ttaggttaaa agacatcagt tgaataaaca ttcacagaga
     3901 cttttatgac acgcgttcaa tttaaacagc accatcatca ccatcatcct gactagtctt
     3961 tcaggcgatg tgtgctggaa gacattcaga tcttccagtg gtgcatgaac gcatgagaaa
     4021 gcccccggaa gatcaccttc cgggggcttt tttattgcgc ggttgataac ggttcagaca
     4081 ggtttaaaga ggaataagaa aatgacagac aacactcgtt tacgcatagc tatgcagaaa
     4141 tccggccgtt taagtgatga ctctcgcgaa ttgctggcgc gctgtggcat taaaatcaat
     4201 cttcacaccc agcgcctgat cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg
     4261 cgtgacgacg acattcccgg tctggtaatg gacggcgtgg tagatcttgg gattatcggc
     4321 gaaaacgtgc tggaagaaga gctgcttaac cgccgcgccc agggtgaaga tccacgctac
     4381 tttaccctgc gtcgtctgga tttcggcggc tgccgtcttt cgctggcaac gccggttgat
     4441 gatgcctggg acggcccgct ctccttaaac ggtaaacgta tcgccacctc ttatcctcac
     4501 ctgctcaagc gttatctcga ccagaaaggc atctctttta aatcctgctt actgaacggt
     4561 tctgttgaag tcgccccgcg tgccggactg gcggatgcga tttgcgatct ggtttccacc
     4621 ggtgccacgc tggaagcaaa cggcctgcgc gaagtcgaag ttatctatcg ctcgaaagcc
     4681 tgcctgatac aacgcgatgg cgaaatggaa gaatccaaac agcaactgat cgacaagctg
     4741 ctgacccgta ttcagggtgt gatccaggcg cgcgaatcaa aatacatcat gatgcacgca
     4801 ccgacccaac gtctggatga agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt
     4861 ctgccgctgg cgggtgatca acagcgcgta gcgatgcaca tggtcagtag cgaaaccctg
     4921 ttctgggaaa cgatggaaaa actgaaagcg ctgggtgcca gttcaatcct ggtcctgccg
     4981 attgagaaga tgatggagtg atcgccatga gctttaacac aatcattgac tggaatagct
     5041 gtactgcaaa gcaacaacgc cagctgttaa tgcgcccggc gatctccgct tctgaaagca
     5101 ttacccgcac tgttaacgat attctcgata gcgtgaaagc acgcggtgat gacgccctgc
     5161 gggaatatag cgcgaagttt gataaaacca cggttaccgc actgaaggtg tctgctgagg
     5221 aaattgccgc cgccagcgaa cgcctgagcg acgagctaaa acaggcgatg gcggtggcag
     5281 taaagaatat tgaaaccttc cacactgcgc aaaaactgcc gccggtagat gtagaaacgc
     5341 agccaggcgt gcgttgccag caagtcacgc gcccggtagc ttcagttggg ttgtatattc
     5401 ctggcggctc cgccccgctc ttctcaacgg tattaatgct ggcaactccg gcgcgtattg
     5461 cgggctgtaa aaaagtggtg ttgtgctcac cgccgccgat tgccgatgag atcctttatg
     5521 cggcgcagct gtgcggtgtg caggacgtgt ttaacgtcgg cggcgcacag gccattgccg
     5581 cgctggcgtt tggtacggaa tctgtgccga aagtggacaa aatcttcggg ccgggtaacg
     5641 cctttgtcac cgaggcaaaa cgtcaggtga gccagcgtct ggacggtgcg gcgatcgata
     5701 tgcccgcagg cccgtcggaa gtgttggtca ttgccgacag cggcgcaacg ccggatttcg
     5761 tggcttctga tttgctttct caggctgaac acggcccgga ctcacaggtg attttactga
     5821 cgcctgacgc tgatatggcg catcaagttg ccgaagccgt cgaacgccag ttagcagaac
     5881 tgccgcgtgc cgaaaccgcc cgccaggcac tgaacgccag ccgcctgatc gtgactaaag
     5941 atttagcgca gtgcgtggag atctccaacc agtacggccc ggagcacctg atcattcaga
     6001 cccgcaacgc ccgcgaactg gtcgatggca tcaccagcgc cggttcggta tttcttggtg
     6061 actggtcacc ggaatcggca ggtgattacg cctccggaac caaccatgtt ctaccgactt
     6121 acggctacac cgccacctgt tccagcctcg gactggcgga tttccagaag cggatgaccg
     6181 tgcaggaact gtcgaaagta ggtttctccg cgctggcttc gaccattgaa acactggccg
     6241 ccgccgagcg cctgaccgcc cacaaaaatg ccgttacttt gcgtgttaac gcccttaagg
     6301 agcaagcatg agcaccgtga ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac
     6361 gccgtatcag tcggcgcgtc gtctgggcgg taatggcgac gtctggctga acgccaacga
     6421 atatcccaca gccgtggagt ttcagcttac tcagcaaacg ctcaaccgct acccggaatg
     6481 ccagccgaaa gcggtgattg aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt
     6541 gctggtcagc cgtggcgcgg acgaaggtat tgaactactg attcgcgctt tttgcgaacc
     6601 gggtaaagac gccatcctct actgcccgcc aacgtacggc atgtacagcg tcagcgctga
     6661 aaccattggc gtcgagtgcc gcacagtgcc gacgctggaa aactggcaac tggacttaca
     6721 gggcatttcc gacaagctgg acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc
     6781 aaccgggcaa ctgatcaatc cgcaggattt tcgcaccctg ctggagttaa cgcgcggtaa
     6841 ggcgattgtg gttgccgatg aagcctatat cgagttttgc ccgcaggcat cgctggctgg
     6901 ctggctggcg gaatatccgc acctggctat tttgcgcaca ctgtcgaaag cttttgctct
     6961 ggcgggcctt cgttgcggat ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa
     7021 agtgattgcc ccctacccgc tctcgacgcc ggttgccgac attgcggccc aggcgttaag
     7081 cccgcaggga attatcgcca tgcgcgaacg ggtggcgcaa attattgcag aacgcgaata
     7141 cctgattgcc gcactgaaag agatcccctg cgtggagcag gtttttgact ctgaaaccaa
     7201 ctacattctg gcgcgcttta aagcctccag tgcagtgttt aaatctttgt gggatcaggg
     7261 cattatctta cgtgatcaga ataaacaacc ctctttaagc ggctgcctgc gaattaccgt
     7321 cggaacccgt gaagaaagcc agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg
     7381 agtcagaagt atctttttat cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat
     7441 tttcaggtgg atcgttttga caaactcgcc tttgaaccgg gcgtgatccc gcaactgctg
     7501 aagctgcaaa aagcgggcta caaactggtg atgatcacta atcaggatgg tctgggaaca
     7561 caaagtttcc cgcaggcgga tttcgatggc ccgcacaacc tgatgatgca gatattcacc
     7621 tcgcaaggcg tacagtttga tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc
     7681 gactgccgta aaccgaaagt aaaactggtg gagcgttatc tcgctgagca agcgatggat
     7741 cgcgccaaca gttatgtgat tggcgatcgc gcgaccgata ttcaactcgc tgaaaacatg
     7801 ggcattaatg gtttacgcta cgaccgcgaa accctgaact ggccaatgat tggcgagcaa
     7861 ctcaccagac gtgaccgtta cgctcacgta gtgcgtaata ccaaagagac gcagattgac
     7921 gttcaggtgt ggctggatcg tgaaggtggc agcaagatta ataccggcgt tggcttcttt
     7981 gatcacatgc tggatcagat cgccacccac ggcggtttcc gtatggaaat caacgtcaaa
     8041 ggcgacctct atatcgacga tcaccacacc gtcgaagata ccggcctggc gctgggcgaa
     8101 gcgttaaaaa ttgccctcgg cgataaacgc ggtatttgcc gctttggttt tgtgctgccg
     8161 atggacgaat gccttgcccg ctgcgcgctg gatatctctg gtcgcccgca cctggaatat
     8221 aaagccgagt ttacctacca acgcgtgggc gatctcagca ccgagatgat cgagcacttc
     8281 ttccgttcgc tctcttacac catgggcgtg acgctccacc tgaaaaccaa aggtaaaaac
     8341 gatcatcacc gtgtagagag cctgttcaaa gcctttggtc ggaccctgcg ccaggccatc
     8401 cgcgtggaag gcgataccct gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc
     8461 ttgatactgg ctgcgccaac ctgaactcgg tgaagtctgc cattgcgcgt cacggttatg
     8521 aacccaaagt cagccgtgac ccggacgtgg tgttgctggc cgataaacta tttttacccg
     8581 gcgttggcac tgcacaagca gcgatggatc aggtgcgtga gcgcgagctg tttgatctca
     8641 tcaaagcctg tacccaaccg gtgctgggca tctgcttagg gatgcaactg ctggggcggc
     8701 gcagtgaaga gagcaacggc gtcgatttgc tgggcatcat cgacgaagac gtgccgaaaa
     8761 tgaccgactt tggtctgccg ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag
     8821 gcaaccgcct gtttcagggg attgaagacg gcgcgtactt ttactttgtt cacagctacg
     8881 caatgccagt caatccgtgg accatcgccc agtgtaatta cggcgaacca ttcaccgcgg
     8941 cggtacaaaa agataacttc tacggcgtgc agttccatcc ggagcgttct ggtgccgctg
     9001 gcgctaagtt gctgaaaaac ttcctggaga tgtgatgatt attccggcat tagatttaat
     9061 cgacggcact gtggtgcgtc tccatcaggg cgattacggc aaacaacgcg attacggtaa
     9121 cgacccgctg ccgcgcttac aggattacgc cgcacaaggt gccgaagtgc tgcatttggt
     9181 ggatctgacc ggggcaaaag atccggcaaa acgtcaaatc ccgctgatta aaaccctggt
     9241 cgctggcgtt aacgttccgg tgcaggttgg cggcggcgtg cgtagcgaag aagacgtggc
     9301 ggcgttactg gaagctgggg ttgcgcgcgt ggtggttggc tccactgcgg tgaaatcacc
     9361 agaaatggtg aaaggctggt ttgaacgttt cggtgccgat gccttagtgc tggcgctgga
     9421 tgtccgtatt gacgagcaag gcaacaagca ggtggcggtc agcggctggc aagagaattc
     9481 gggcgtgtca ctggaacaac tggtggaaac ctatctgccc gtcggcctga aacatgtgct
     9541 gtgtaccgat atctcgcgcg acggcacgct ggcaggctct aacgtctctt tatatgaaga
     9601 agtgtgcgcc agatatccgc aggtggcatt tcagtcatcc ggcggcattg gcgacattaa
     9661 cgatgttgct gccctgcgtg gcactggcgt gcgcggcgta atagttggtc gtgcattact
     9721 ggaaggtaaa ttcaccgtga aggaggccat ctcatgctgg caaaacgcat aattccatgt
     9781 ctcgacgttc gtgatggtca ggtggtaaaa ggcgtacagt ttcgcaacca tgaaatcatt
     9841 ggcgatatcg tgccgctggc aaaacgctac gccgaagaag gtgcagacga actggtgttc
     9901 tacgatatca ccgcttccag cgatggccgt gtggtagata aaagttgggt atctcgcgtg
     9961 gcggaagtga tcgacattcc gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc
    10021 gcgaaaattc tttcctttgg cgcggataaa atttccatca actcccctgc gctggcggac
    10081 ccgacgttaa ttactcgcct ggcggatcga tttggcgtgc agtgtattgt ggtgggtatt
    10141 gatacctggt acgacgctga aaccggtaaa tatcatgtga atcaatatac cggcgatgaa
    10201 agccgcaccc gcgtcactca gtgggaaacg ctcgactggg tacaggaagt gcaaaaacgc
    10261 ggtgccggag aaatcgtcct caacatgatg aatcaggacg gcgtgcgtaa cggttacgac
    10321 ctcgaacaac tgaaaaaagt gcgtgaagtt tgccacgtcc cgctgattgc ttccggtggc
    10381 gcgggcacca tggaacactt cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg
    10441 gcagcttccg tattccacaa acaaataatc aatattggtg aattaaaagc gtacctggca
    10501 acacagggcg tggagatcag gatatgttaa cagaacaaca acgtcgcgaa ctggactggg
    10561 aaaaaaccga cggactgatg ccggtgattg tgcaacacgc ggtatccggc gaagtgttaa
    10621 tgctgggcta tatgaacccg gaagccttag acaaaaccat cgaaagcggc aaagtcacct
    10681 tcttctcgcg cactaaacag cgactgtgga ccaaaggcga aacgtcgggc aatttcctca
    10741 acgtagtaaa tattgcgccg gactgcgaca acgacacgct actggtactg gcgaatccta
    10801 tcggaccaac ctgccacaaa ggcaccagca gctgcttcgg cgacaccgct caccagtggc
    10861 tgttcctgta tcaactggaa caattgctcg ccgaacgcaa atctgccgac ccggaaacct
    10921 cctacaccgc caaactgtat gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag
    10981 aaggcgtgga aaccgcactg gccgcgactg tacatgaccg ctttgagctg accaacgagg
    11041 cgtctgactt gatgtatcac ctgctggtat tgttgcagga tcaggatctg gatttaacaa
    11101 cggtgattga gaacctgcgt aaacgtcatc agtgagttgc ggggtaagcg gatgcgatat
    11161 tgttgtcgca tccggcaaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa
    11221 aaccgaaaag attacttcgc gttgtaatta cgcagagcgt tacgccccag cacaatcccc
    11281 gcgccaacca tgccgcccag cagcaccgcc agaatcaagg tgattgcctt tttcggacta
    11341 tcgcgacgaa taggtaacgt cggtttcatt acatagcggt aagcatgaat atcaagatca
    11401 tcaaccttta agttttcaat atccaacagg ttttgacgtg tctgatagta gtttggtgag
    11461 aacaccaacg gacgggtcgc ctcatgctta atcatcgact ccagcgcttc gctccccaaa
    11521 aggaacaacg tatcttgcgt cacatcttca gtctgctgaa cctgtggctt tgccacctgc
    11581 gcctgattcg catactgcaa cgcttcctga atctgacgga tacgcagctc tttctgctcc
    11641 tgcgcaacta cttcctgcgt tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg
    11701 ttgtctttga gatccttttc tagctcttga ttcactttat catcaacttg ctgaatgtat
    11761 tgggccaact tcatttgtgc tccctctgca gtttgcccaa cataagaaac agtcaatggt
    11821 aattgctggt tcttaacaga aggttcgatg gtaagttttt ctggttcttc ctgattatcc
    11881 agcgtttctg ctaatgctga gaaggcagaa ctgaagcgac caatcaacgt ctcctgcaaa
    11941 tccgatactt tcggtgcagc ctgaccatag ataacattca tggcattgtt atagccagca
    12001 atttgcccca cgtcgggctg agtgataatt gctgttgacg tccatttctc cttcgctact
    12061 gccaaataac caatagccag agcaatagcc acaatgacgg aaatgatgat tgtcatcttg
    12121 ccacgccaca actgcactag taaatcaatc aaatcaatct gttccgggtc atggttttgc
    12181 ccagaaacat tattattttc tactctcatc attaccctaa ctgacggaaa tttccaagtc
    12241 cgcatagttt agcgataatc cctgtgaatg ctataggata agtgataaaa ataatgaatt
    12301 attgcggagg taacaaaaga caatcagggc gtaaatagcc ctgataacaa gatgttagtc
    12361 gctgccaaag agatcgcggg tgtagacctt atctgccaca tccctaagct cttctgccat
    12421 acggttggaa ataatgacgt cggcttgttg tttgaaggtg gcgagatcac gttccaggcg
    12481 agagttgaag aatgagtctt ctttcatcac tggctcgtag atgattactt caacaccttt
    12541 cgccttgata cgcttcataa tcccctgaat agaagacgca cggaagttat ctgaaccgct
    12601 cttcataatc agacgataaa tacctaccac ttgcggcttg cgtgacaaaa tggcatcggc
    12661 aataaaatct ttacgcgtgc ggttagcatc gacaattgcc gagatcaggt tattcggcac
    12721 agactggtag ttcgccagta actgcttggt atctttcggc aaacaataac caccataacc
    12781 aaacgacgga ttgttgtaat ggttgccaat acgtgggtcg agacaaacgc cttcgattat
    12841 ttgacgggaa ttcagaccta aactttctgc gtagctatcc agttcgttaa agtacgccac
    12901 gcgcatcgcg aggtaggtgt ttgcaaaaag tttaatcgct tctgcttcag tggagtcggt
    12961 aaacagggtt gggatatttt gcttaatcgc gccttcctgt aataacgcag caaaacgttc
    13021 tgcgcgttcc gaacgctcac cgattacaat acgtgacgga tgaaggttat cgtaaagagc
    13081 tttaccttcg cggagaaatt ccggagagaa aataatattt tcagtacgat atttcttatg
    13141 catcgcttcg gtaaaaccaa cgggaaccgt tgatttgatg accataaccg cataaggatt
    13201 tatctcaact acgtctttaa ttactgattc tacactggat gtattgaaat aattagtttt
    13261 aggatcatag tcggttgg
//
LOCUS       DAZKFZ010000118        13251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000118 DAZKFZ010000000
VERSION     DAZKFZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..2804
                     /gene="evgS"
                     /locus_tag="ACHOQW_003078"
     CDS             <1..2804
                     /gene="evgS"
                     /locus_tag="ACHOQW_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP9574120.1"
                     /translation="NEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLE
                     NPYSPPYSMTDENGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDIL
                     PGAIYSEDRENNVLFAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKE
                     MYPEVEWIKVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNA
                     SLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVT
                     TLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNV
                     ISHNSAFEHYFTADYYKNAMLPLENSESPFKDVFSNTHEVTAETKENRTIYTQVFEID
                     NGIEKRCINHWHTLCNLPASEHAVYICGWQDITETRDLIHALEVERNKAINATVAKSQ
                     FLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVD
                     KIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFK
                     QVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRY
                     SQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEIIQQVAAVE
                     AKAEQPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYD
                     LLITDVNMPNVDGFELTRKLREQNSSLPIWGLTANAQANEREKGLNCGMNLCLFKPLT
                     LDVLKTHLSQLHQVAHIVPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAA
                     FHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSV
                     KEHIAELDQEIAVFCQQNN"
     gene            complement(2860..4005)
                     /gene="yfdE"
                     /locus_tag="ACHOQW_003079"
     CDS             complement(2860..4005)
                     /gene="yfdE"
                     /locus_tag="ACHOQW_003079"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP9574121.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGLSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQTILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4079..5023)
                     /gene="yfdV"
                     /locus_tag="ACHOQW_003080"
     CDS             complement(4079..5023)
                     /gene="yfdV"
                     /locus_tag="ACHOQW_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP9574122.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5093..6787)
                     /gene="oxc"
                     /locus_tag="ACHOQW_003081"
     CDS             complement(5093..6787)
                     /gene="oxc"
                     /locus_tag="ACHOQW_003081"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP9574123.1"
                     /translation="MSDQLQMTYGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6841..8091)
                     /gene="frc"
                     /locus_tag="ACHOQW_003082"
     CDS             complement(6841..8091)
                     /gene="frc"
                     /locus_tag="ACHOQW_003082"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP9574124.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(8604..9239)
                     /gene="yfdX"
                     /locus_tag="ACHOQW_003083"
     CDS             complement(8604..9239)
                     /gene="yfdX"
                     /locus_tag="ACHOQW_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP9574125.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            9535..9810
                     /gene="ypdI"
                     /locus_tag="ACHOQW_003084"
     CDS             9535..9810
                     /gene="ypdI"
                     /locus_tag="ACHOQW_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP9574126.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSNSGGGSETSYKEPEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(9887..10129)
                     /gene="yfdY"
                     /locus_tag="ACHOQW_003085"
     CDS             complement(9887..10129)
                     /gene="yfdY"
                     /locus_tag="ACHOQW_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP9574127.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            10482..11402
                     /gene="lpxP"
                     /locus_tag="ACHOQW_003086"
     CDS             10482..11402
                     /gene="lpxP"
                     /locus_tag="ACHOQW_003086"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP9574128.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            11758..11829
                     /gene="ypdK"
                     /locus_tag="ACHOQW_003087"
     CDS             11758..11829
                     /gene="ypdK"
                     /locus_tag="ACHOQW_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP9574129.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(11894..13132)
                     /gene="alaC"
                     /locus_tag="ACHOQW_003088"
     CDS             complement(11894..13132)
                     /gene="alaC"
                     /locus_tag="ACHOQW_003088"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP9574130.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         3703 a         3126 c         2860 g         3562 t
ORIGIN      
        1 caaatgaagt tcgctatgaa gtatcacaaa attggcttga tacaggaaac ctggcctttc
       61 tgaacaagcc attagaactc actgaacatg aaaaacagtg gattaagcag catcccgatt
      121 taaaggtgct ggaaaatcct tactcgcccc cctattctat gacggatgaa aatggctcgg
      181 ttcggggcgt tatgggggac attcttaata ttattacctt gcaaacaggt ttaaattttt
      241 ctccgatcac cgtttcacac aatatccatg ctggaacaca gctgaatccc ggcggatggg
      301 atatattacc cggtgctatt tatagtgaag atagagaaaa taatgtttta tttgctgaag
      361 ccttcataac aacgccttac gtttttgtca tgcaaaaagc gcctgacagt gaacaaacat
      421 taaaaaaagg aatgaaagtt gccattccat attattatga gcttcattcg caattaaaag
      481 agatgtatcc ggaggtagag tggataaaag tcgataacgc cagcgctgca tttcacaagg
      541 tcaaggaagg cgaacttgat gctctggtcg cgacacagtt aaattcacgt tacatgatcg
      601 accattacta tcctaatgaa ctgtatcatt ttcttattcc cggcgttccg aatgcatcgc
      661 tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga
      721 atgcaatccc cccaagcgaa gttctgcgcc tgaccgaaaa atggattaaa atgcccaatg
      781 tgaccattga cacatgggac ctctatagcg agcaatttta tattgttacg acattatccg
      841 ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc
      901 gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccggaaagcg ctctcggact
      961 ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cacaatagtg
     1021 catttgaaca ttatttcact gctgattact acaaaaatgc aatgttgcca ttagaaaaca
     1081 gtgaatcacc ctttaaagat gttttttcta atacgcatga agtcacagca gagacgaaag
     1141 aaaaccgaac aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca
     1201 ttaatcactg gcatacatta tgtaatctgc cagccagcga acatgctgtt tatatttgtg
     1261 gttggcaaga tattaccgag acgcgtgatt taattcatgc actcgaagta gagagaaata
     1321 aagcgatcaa tgcaactgtc gcaaaaagtc agtttctggc aacaatgagt cacgaaataa
     1381 gaacaccaat aagctccatt atgggcttcc tggaacttct gtcgggttct ggtcttagca
     1441 aggagcaacg ggtggaggcg atttcactag cctacgccac cggacaatca ctcctcggct
     1501 taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac
     1561 aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg
     1621 caagcaaatc gatcgcatta agttgcagca gtacatttcc tgatcattac ctggttaaga
     1681 tcgaccctca ggcgtttaag caggtcttat caaatttact gagtaatgct ctcaaattta
     1741 ccaccgaggg ggcagtaaaa attacgacct ccctggttca cattgatgac aaccacgctg
     1801 taatcaaaat gacgattatg gattctggaa gtggattatc acaggaagaa caacaacaac
     1861 tgtttaaacg ttatagccaa acaagtgcag gtcgtcagca aacaggttct ggtttaggct
     1921 taatgatctg caaagaatta attaaaaaca tgcagggtga tttgtcatta gaaagtcatc
     1981 caggcatagg aacaacattt acgatcacaa tcccggtaga aattatccaa caagtggcgg
     2041 ctgtcgaggc aaaagcagaa caacccatca cactacctga aaagttgagc atattaatcg
     2101 cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg
     2161 atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg
     2221 atctgcttat tactgacgtt aatatgccga atgtggatgg ttttgagttg actcgcaaac
     2281 tccgtgagca aaattcgtcc ttacccatct gggggcttac agccaacgca caggctaacg
     2341 aacgtgaaaa agggttaaat tgcggcatga acttatgttt gttcaaaccg ttgaccctgg
     2401 atgtactgaa aacacattta agtcagttac accaggttgc gcatattgta cctcagtatc
     2461 gccaccttga tatcgaggcc ctgaaaaata atacggcgaa cgatctacaa ctgatgcagg
     2521 agattctcat gactttccag catgaaacac ataaagattt acccgctgcg tttcatgcac
     2581 tagaagctgg cgataataga actttccatc agtgtattca tcgcatccac ggtgcggcta
     2641 acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata acacctgttt
     2701 cagatgacag taagcctgaa attcttcagt tgctaaactc tgtaaaagaa cacattgcag
     2761 agctagacca ggaaattgct gttttctgtc aacaaaataa ctaaatagtg gctcccacaa
     2821 tgttcaaatg tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg
     2881 aatttgctcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca
     2941 gccactgatt tttatcggat tacccggcat cattattccc ccggcttcaa tcaacatatt
     3001 tctcgcctga gtttgtggca aattaatggc ctcagccaca cttaataacg gcgcgacggg
     3061 cacaccaact tcatgtattc tggctaacca aacttcagct gcctgtgttt ttaacgtccg
     3121 ctcaatatat tgtttaagaa tagtctggtt ttgtacgcgt aaaatattgc tgctaaatct
     3181 gggatcatta accagttccg taagctccag tgcctggcat aacgcagaga aaagcttgtc
     3241 attaccacaa caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa aaggtgccat
     3301 gtaggggtgg cgatttccca ggcgttgtgg tgacttccct gtcgcaatat atgccatcag
     3361 accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct
     3421 ttggctcttt tcgcggccat aaagtgcact cactattccg ctgaataaat aaacaccgcc
     3481 gcatagatcc gcaagagagg taccaacacg cactggcgga gcatcagggt accctgtttc
     3541 catcattatc ccgctcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg
     3601 accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga tttcttgtaa
     3661 cctttcccat gaaagcccca gtttttccat tgtacctggg cgaaaattct cagctaatac
     3721 atcagcttgt ttaagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag
     3781 aaccacactc tctttaccat gattaataaa actgtaatag agtgactgtc catccacata
     3841 gggaccaaat gtgcgggtat catcaccatg acccggcggc tcaactttaa ttacccttgc
     3901 gcccatatta caaagaagtt gagttccgaa aggtccatta aggacatgtg tcatatcgat
     3961 aactaataag ccttcaaacg gccctttgct ttcattattt gtcatttgct tatgccttga
     4021 aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt
     4081 atgaaaccag tcgactgaca taaatccaca agggagccgt gacgacaaaa cccagtacgc
     4141 tcacagccaa tgacgctgta ccagtgcggg tgtagacatt aaaccggctg gcaataatga
     4201 ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaga tgttcgctgt
     4261 tcaaatgaca tgccatacca acaagaagca gcgccagtgg catcagaatc agcttcagga
     4321 aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg
     4381 cagcgaatac cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg
     4441 ctgccggaat ttttacccca accaacacca ggatcgttgc cagaacaggt gcccatacaa
     4501 ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc
     4561 ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta acaataatag
     4621 aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga tcgagaactg
     4681 caaaccccag gaatccaatg gtaggtgaac ctgcaattaa tgcacataca gctgcttctg
     4741 catgggtacg tttaaaaaat ttgtagcaac caaaccagga gaaaaagaaa catccgacaa
     4801 taaccacgag tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc ctgtttgccc
     4861 gggtaataga aacaaatagg gctgcaggaa gcgcgtagtt taataccagt ttattaaatg
     4921 cccgggcttg atcttctgaa aaagtttctc gtctgccgct aaaataaccc aacagcatga
     4981 ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta tttccgataa
     5041 gggtcccctc accatcgcat aatgagttgg caagaggaga cgatgtcagt atttaattac
     5101 cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct gctgcagggt
     5161 cgatgaccac attaataatg gtcggtttgc gcgactgaat accggtggtt aaagcatgac
     5221 gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc attaatttgt
     5281 cataccttgc atggtgcaac agatctgttg gtgatggtgc accagcgcca ctgagatcaa
     5341 caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgttacc ggcaggttat
     5401 atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa agcactatca ccttcaatgg
     5461 cgacaaccgg agaaccagag gtcacgctag caccgatggc atagcccata ccgatgccca
     5521 tgacacccca ggtaccacaa tccagacgac gacgtggttt atacatatca ataatatttc
     5581 gtgcattatc cagggtattt gcaccttcat taactaaata aatatcctgg ttctcacgca
     5641 atacatcgcg cacagcactt aatgcattaa agtaatttaa tggttgggta tctgtactta
     5701 atttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata tcgcgccata
     5761 ccagtggagt cgtaaatgtg ttttgtttca gctctgccag cataccttgc atactggatg
     5821 caatatcgcc aacgactggc acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa
     5881 tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt gccagtaacc
     5941 aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca aacgaacgcg
     6001 cagctgccgc agaaagtgga tgcgtatctt cgaggatccc tttcgccata gacattggca
     6061 ggaatggaat ctgggcactt tcaataaatt cacgaagctg ttcgtcagct tgtgaatacg
     6121 ccgcgccttt gccaaggata attaatggcc gttcagcttt tgctaaaagc gaaattgcgc
     6181 tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact tttacaatcg
     6241 tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc aaatcaagat
     6301 aaactccgcc agggcgaccc gatactgaga cccggatagc gcgtgccaat gcaatgccaa
     6361 gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc gcattcattt
     6421 ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg ctggagccgc
     6481 taatcatgat catcggaaaa ccatttaccg ttgcgttggc caatgcggtc aaaccattga
     6541 ggaatcctgg cgcagacact gtcaggcaga tccctggttt ttgggtaaga aaaccgcttg
     6601 ccgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga atgccttccg
     6661 cctgggcatg gcgcgccata tccgtcacag gaatacctac aacaccataa atagtgtcaa
     6721 tattattctg ttttaatgct tcaacgatga tatgcatacc atatgtcatt tgaagttgat
     6781 ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc cccgttaata
     6841 tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac ccagctcctg
     6901 caataccgca gcggtatgtt cacctaatag aggcgcagct ttaatatccg gcgtaaaggc
     6961 agagaatttc attggacaac caactgtcag atattttcca cgcaatggtt gttccacttc
     7021 gacaacactg ccgctttggc gtaaagaggg atcaagtgaa atttctttca tacttaaaac
     7081 cggtgcacaa ggaatatcaa actgagtcaa ataagccact gcttcatgtt tatcaatagt
     7141 gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc gggcatgggc
     7201 tgtactgtat gccggatcgg taatccaatc tggtttgccg atggctttac aggtgttttc
     7261 ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcagttt cccagccttt
     7321 acatttcagg atccagccag gctgaccgcc accacccgca ttacctccgc ggggaactgc
     7381 atcaccaaat gtgccattcg gatactgcgg gtattcttcc agataaccca atttatcgag
     7441 acgctgctgg tcgcgtaatt tcacgcggca aaggttcaat acggcatcct gcattgacat
     7501 ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat
     7561 cagtaaatgc attccggtgt tgctgtcacc caacgctgca gcgcttacca gcggcggacc
     7621 gtcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt tttcataggc
     7681 ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa aaatcagacg
     7741 tggattgatt tcttgaatat gctcccaggt gaagcccatg tgatcaatgg cccctggatg
     7801 aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct ctttgccttc
     7861 cgctgttttg gtatttaact caatagaacg tttgttactg ttaagcatgg tgaagtaaag
     7921 cgcatcgata tcaggaatat ctcgcagctg gtgacgcgtt acgtcaccaa cgccggggcg
     7981 ttcaatttta atgacgtcag cgccaaacca ggccagcatt tgagtacaag atgggccaga
     8041 ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttgaca tagcaatacc
     8101 tattagttaa ttaaatatgt gttaatttag cgtttattta aggcataacg ccctctataa
     8161 ataatccgca aaatatcgtc atagcggcac tcgatatttt taaattcctg tatcaagtgc
     8221 tgaaaatcat ttcatcgcta cttccaattc ctttctgcat ggtaagtagt gaggacgaaa
     8281 agaagaatat gataaatatt ggttcgtggt aatcagagag agtcgcgaca tttgtgatgt
     8341 tattcttaca actgtaagaa gcgccggtga tttacaagta agggtatgat tacgcttttt
     8401 agagcgtacc ataaaatata acatattaaa tgatttgtga tgacacggga ttattgcggt
     8461 ttactttgag gaatataaaa tgcgagttta tagaacagcg agcgccaaat aattttatga
     8521 ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta
     8581 cctcaatata ctgcctgtca gcattagttc acaaacagtg cttcgctatc gacgatgatt
     8641 ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt
     8701 ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg gcatcagata ttggttttcc
     8761 attacaccaa caccggccag gcgaagctct tccattgcgc cctttttatc ccctttcgcc
     8821 attttttcat tggcaatttt tatcgcggct tctttttcgg gtgtcgctac ataactctca
     8881 gaaattccga cagatgcatt aatgacaata tattggtcat cattgacatt ggtcttttta
     8941 cctggcttag cgaactttgc ccattcggta ctgtcatctg acaacaaagc tgaagcttca
     9001 ttggtcagtt cttttgcctt ttccggatcg ccatgaaaca gagccaggcg cgctacctgc
     9061 acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgcagc tgccgttttc
     9121 tgggtttgct gaacttgctg agtttgctgt tgtgccgcta ccggagcatt atcagcagcc
     9181 catacagttg aagatgccag aatagctgtg accatcgtag ccataattaa acgtttcata
     9241 cttacctcat tctttaacag ttttagaaaa cgccgccatc atattgggtg agcatgaaat
     9301 gagaaatcag agagcaacgg tcattttgaa agggttatct tacagttgta gttattatcg
     9361 caagaagtat tttagtctta caattgtagg aatatgcttc ctgtcacaga gaactattct
     9421 tatgtaccac aaagagatgt catttattat atcgatgtaa atattgatta attatatgta
     9481 aatacagcca taacatcgta cattctcagt gaaccatttt caggataact ttctatgaaa
     9541 gtaaacttaa tactttttag cttattttta ttggtctcta ttatggcatg caatgttttt
     9601 gcattttcta attctggtgg tggtagtgag acgagctata aagagccaga aaaaacgtca
     9661 gcgatgacga caactcactc tacaaaattt cagccatcac aggcgatttt gtttaagatg
     9721 agagaagatg caccaccatt aaacctcaca gaagaaacca cgcccccttt tccgacaaag
     9781 gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac ctggcaggtg aacaatcatg
     9841 tctgatgctg atgtgaacac ctgcccaaat gaaccatcct gacccactat ctcctgccat
     9901 accccaccat aaagcgaatc atcaagccac aagccggggc aagaaacata aatatccccg
     9961 tcaccagttc attaccgcca gttaaccaca tggtgaagta ataaatgagt gcagcgagga
    10021 tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt cagtacctga ccgatgtatc
    10081 cgcttacgca gacaatacac agggcgagaa acatccataa ataaaacata gctcaacctc
    10141 ctgccgcgct aaaaaacctg taacttcagt cactttaata gtaagacaat ccttaacctc
    10201 tggcaatttt tgctaagcgt caatgaaaac agcatgttaa atgcaatact attgcgtgcg
    10261 gaaaattttt tactttgcac gattaataat cagtagctga aagcagtcag ccagtgataa
    10321 agtacagata ccgcattcat gctcctcgtg gttatgtcct gactagtctt tacactcttt
    10381 acaggaacca ttgtcgtaca tgatggccca accaattgag tgtaccgctg tcgacatgga
    10441 atcttctgcg atacaacaat tcgtatctac agaaggtaac tatgtttcca caatgcaaat
    10501 tttcccgcga gtttctacat cctcgctact ggctcacatg gtttgggctt ggtgtactct
    10561 ggctttgggt acagcttcct tatcctgttc tctgctttct cggcacgcgt attggcgcaa
    10621 tggcgcgacc attcctgaaa cggcgtgaat ctatcgcccg taaaaacctg gaactttgct
    10681 tcccgcagca ttctgcggaa gaacgcgaga agatgattgc cgaaaacttt cgttcactcg
    10741 gcatggctct ggtagaaacc ggcatggcgt ggttctggcc cgacagtcgc gtacgtaaat
    10801 ggtttgatgt tgaagggctg gataacctta aacgcgcaca aatgcaaaat cgcggcgtaa
    10861 tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg ccgcgtgatg ggactttgtc
    10921 agccaatgat ggctacctat cgtccacata ataatcagct gatggaatgg gtgcagaccc
    10981 gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa taatctgcgc ggcattgtcg
    11041 gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga tcaggattat ggtcgtaaag
    11101 gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc cacaaccaat ggcacctatg
    11161 ttctctcccg tctctctggc gcagccatgt tgaccgtaac gatggtaaga aaagcggatt
    11221 acagcggata tcgtttgttc atcaccccag agatggaagg ctacccgaca gatgaaaatc
    11281 aagccgctgc ctatatgaac aagattatcg agaaagagat catgcgcgca ccggagcagt
    11341 acctctggat ccaccgtcgc tttaaaacgc gcccggtggg agaatcgtcg ttgtacattt
    11401 aacaaactgc tcttactctt tccctcctca aatcggccat aataacctca tggtttttat
    11461 ggcttttttc ttaagatttt ttccttcgct gaatatctaa ttattaccgc cagaaattaa
    11521 actgtcgccg atatgagaca cctgtaagtt actaaaagta aataagaaaa tgcctcttgt
    11581 ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc agtttagctc agaattctgg
    11641 ggtgatgccc acggtaaaat acaggacttt taaggtcgtt ttgcccggtg tcaactcact
    11701 gtatcaggtg tgatgaagtc attcaggcgt aaccgtaatt acgcggagag atgtaaagtg
    11761 aaatatttct ttatgggcat ttcttttatg gtcatcgttt gggccggtac ttttgccctg
    11821 atgatctaaa agcaaactgt gcaatgaaaa acaggagcca tcaggctcct gtttgcattt
    11881 ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt gctgctggcg ggtagtaaac
    11941 cgtcggcacg gaacatcgct ttaatcccac gaatcgcctg acgaatacgg tcgcggtttt
    12001 caatcagtgc aaagcgaaca tgcgtgtcgc cgtagtcacc aaagccaatc cctggagaga
    12061 cacagacctt cgcttcgtta aggagcttct tggcaaattc cagcgatccc atggccgcat
    12121 atggttccgg gattttcgcc cagacataca tcgaagcctt cggcatttcg accatccagc
    12181 ccgcttcatg cagcccttta accaatacat cgcggcggcg tttgtactgt tcggcaatgt
    12241 cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc cgccacctgc aacggcgtaa
    12301 aagtgccgta atcgtgatag cttttgattc gcgccagggc actgaccagc gttttgttgc
    12361 cgaccataaa gccgatacgc cagcccgcca tgttgtagct tttcgacagc gtaaagaact
    12421 cgactgccac atcgcgtgca cccggtacct gcatgattga cggcgctttc cagccatcgt
    12481 agacgatatc ggcataggcc aggtcatgga ccaccagcac atcgtagcgt ttcgccagcg
    12541 ccactacttt ttcgaagaac tccagttcca cgcattgcgc ggttgggttt gacgggaagc
    12601 cgaggatcat catcttcggt ttcggataac tttcaagaat ggcgcgctcc agttcgttga
    12661 agaaatcgac accttccacc agcggcactg agcgtacctg cgccccggca atcaccgcgc
    12721 cataaatatg gatcgggtag cttggattcg gcaccagcac cgtgtcacca tgatccagcg
    12781 tcgccagcat caggtgtgcc agcccttctt tcgaaccaat ggtgacgatg gcttctgatt
    12841 ccggatcgat ttcaacgtcg tagcgatcct gataccagcg ggaaatggcg cgacgtaacc
    12901 gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtccgg acgctgggcc acagtacata
    12961 atttttcgac gatatgcggc ggagtcgcac cgtccgggtt acccatgctg aaatcgataa
    13021 tatcttcgcc gcgccgacgc gcagccattt tcagttcagc ggtgatgtta aaaacgtagg
    13081 gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg agtgtcagcc ataatttcct
    13141 cagattaacg ttagcgcccg gaccgtccga gcgacgctgc cacgatggtg gctgttttga
    13201 aaatagcctg attaatttct gtctgtccag cgttttagaa catttttatt a
//
LOCUS       DAZKFZ010000119        13128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000119 DAZKFZ010000000
VERSION     DAZKFZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..256)
                     /gene="ydiV"
                     /locus_tag="ACHOQW_003089"
     CDS             complement(<1..256)
                     /gene="ydiV"
                     /locus_tag="ACHOQW_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP9574131.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLN"
     gene            complement(503..967)
                     /gene="nlpC"
                     /locus_tag="ACHOQW_003090"
     CDS             complement(503..967)
                     /gene="nlpC"
                     /locus_tag="ACHOQW_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9574132.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(1045..1794)
                     /gene="btuD"
                     /locus_tag="ACHOQW_003091"
     CDS             complement(1045..1794)
                     /gene="btuD"
                     /locus_tag="ACHOQW_003091"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP9574133.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1794..2345)
                     /gene="btuE"
                     /locus_tag="ACHOQW_003092"
     CDS             complement(1794..2345)
                     /gene="btuE"
                     /locus_tag="ACHOQW_003092"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP9574134.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(2408..3388)
                     /gene="btuC"
                     /locus_tag="ACHOQW_003093"
     CDS             complement(2408..3388)
                     /gene="btuC"
                     /locus_tag="ACHOQW_003093"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP9574135.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(3489..3788)
                     /gene="ihfA"
                     /locus_tag="ACHOQW_003094"
     CDS             complement(3489..3788)
                     /gene="ihfA"
                     /locus_tag="ACHOQW_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP9574136.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3793..6180)
                     /gene="pheT"
                     /locus_tag="ACHOQW_003095"
     CDS             complement(3793..6180)
                     /gene="pheT"
                     /locus_tag="ACHOQW_003095"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9574137.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVETLLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(6195..7178)
                     /gene="pheS"
                     /locus_tag="ACHOQW_003096"
     CDS             complement(6195..7178)
                     /gene="pheS"
                     /locus_tag="ACHOQW_003096"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9574138.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(7387..7511)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(7461..7505)
                     /gene="pheM"
                     /locus_tag="ACHOQW_003097"
     CDS             complement(7461..7505)
                     /gene="pheM"
                     /locus_tag="ACHOQW_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP9574139.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(7628..7984)
                     /gene="rplT"
                     /locus_tag="ACHOQW_003098"
     CDS             complement(7628..7984)
                     /gene="rplT"
                     /locus_tag="ACHOQW_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9574140.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(8037..8234)
                     /gene="rpmI"
                     /locus_tag="ACHOQW_003099"
     CDS             complement(8037..8234)
                     /gene="rpmI"
                     /locus_tag="ACHOQW_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9574141.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(8331..8873)
                     /gene="infC"
                     /locus_tag="ACHOQW_003100"
     CDS             complement(8331..8873)
                     /gene="infC"
                     /locus_tag="ACHOQW_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9574142.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8877..10805)
                     /gene="thrS"
                     /locus_tag="ACHOQW_003101"
     CDS             complement(8877..10805)
                     /gene="thrS"
                     /locus_tag="ACHOQW_003101"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9574143.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFILYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            11328..>13128
                     /gene="espL1"
                     /locus_tag="ACHOQW_003102"
     CDS             11328..>13128
                     /gene="espL1"
                     /locus_tag="ACHOQW_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFP9574144.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNSSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIH"
BASE COUNT         3342 a         3432 c         2978 g         3376 t
ORIGIN      
        1 aattaagcca ggcaaagagt ttatgttgaa taaaaaaatg ttggcatgat tttagtaatt
       61 ccaactgctc tgaaaataat tgccaatgtt gctcctcggt aagttgcgct ataacccgac
      121 tcgtcgggat acgcaccgtg ccatcttcac tggagaaatg ggtaattaac tcaacgccga
      181 caaggacttg ctggttatct cttatcggca ggaaatagca atcagaatga taaagattct
      241 ccaaaaaaat cttcatggta accgtccctc tcgaaggatg tttttcagta tccggctaaa
      301 agggatgagg ccataagaca taacaattac agaaggagta actttcattt gttccatgtt
      361 aaccactttt tcaggggtcc tttttttaga ttatcctgat tataaacgaa taatccattt
      421 acggaatttt tgtctgcaaa atactactgt attctggagt aaatggacgg cgatgtatca
      481 tcgacgtcca attttcgaca tactagattc gtctcgcctg ccagaaattt ttctgccaat
      541 agacattatc aagtgaggaa cgcatcactc ccttgctggt agaggcgtgg ataaattggt
      601 tgttggtatc gtaaatacct acatgcaaac cattttgtcc ggaacccgtt ttgaaaaaga
      661 ccaggtcacc aggcagcaac tcgtctttat caatttgcgt gccgatagag gcttgttctt
      721 tggtttctcg gggcagctgc aaatcgaaac gatcgcgcat cgtcacaacc acaaatcccg
      781 aacagtccac accgcgccgc gtcatgccac cataacgata cggcgtgcca tgccagcttt
      841 gtagctggtc gttcaaaccg gcaataacgg taatcgaatc agaaagtctg gcatttggcg
      901 gcggtgcttt atggtggcta cacccggcca gaaacagtgc tgtgatcaaa ataaggcaga
      961 aacgcattcc gtacggttcc tctgtttttt attcttgcat taatttagcg tcgtaattac
     1021 ccgattttca agatactaat gaaatcagat ggtcgaaatc agcattctgt gaccttcgat
     1081 atccagacgg cgaaaattca tcccataggc ctgcgccaga tttggcggcg tgagcacatc
     1141 ttctctgcgt ccactggcca gcatttttcc accttttagc aaccacgccc gatgcgcatg
     1201 acgcaatgtg tggttgagat cgtgactgct catcacaatc gccagtcctt gctgacacag
     1261 cgcgctcaga attttgtcta acgcactttg ttgcgcaaca tcaagactgt tcatcggctc
     1321 atcaagaagc agcaattggc ctgcgggatt ggcttgtggt gtgatttgca gcaccaccgc
     1381 agcaagacgt acgcgttgcc attcaccgcc ggaaagttga ttggtgctac gaccgagttt
     1441 gtcatcaaga gccagcgccc ctgcgacatc attcagtagt tcggtacgcg ttttatcgtg
     1501 ctgatgcagt gtcaggtagt gccagaccgg cattgcaaac ggcggcgtct gctgttgtga
     1561 aagataggca cgatgcagcg cgagttttgt tgcggaccat gcttccagtg gttgccccgc
     1621 gaactgaatg cttcccttac cgctggtcat tccggccatt cgcgccagta aggtactctt
     1681 acccgcgcca ttcggcccca ccaggtgcag gatctcccca gcccgaacct cgccagaaag
     1741 cggccccagg cgggtagatt ccgcaacatc ctgtaactgc atcacaatag acattatttt
     1801 gccaacgcca gtttaatgct ttccatcaca atgggatctt ccggcgtcat atccggggaa
     1861 aaacgctgga tgaccaatcc gtccctgcca actaaaaatt tctcaaaatt ccataaaata
     1921 tcatcagggt acagcggtgc acgacctttg ctgaccatac gggcatagaa tccgctctct
     1981 tccggcgcga ctgcggtcgg cgctgcggaa atcaattttt gatacagcgg atggcgtcct
     2041 tcgccattaa cttcaatctt actgaacatc gggaacgtca ccccccatgt ggtggtacag
     2101 taagttttaa tctcttcatc gctgcccggt tcttgttcca gaaactggtt gcacgggaat
     2161 cccagcacca caaaacctcg atcggcccag gctttctgaa tattctccaa ctgctcatat
     2221 tgcggcgtta agccacactt tgaggcgaca ttgacaatca acagcacatt accggcgtac
     2281 ttctccagcg tggtcacttc accgtcgata tctttcacta cggtcgtcag aatggaatct
     2341 tgcatcgttt ctcctgggtg tggtcagtaa aaatcttagc ttttaatcat agaccgtctt
     2401 tttgcggcta acgtcctgct tttaacaata accagataaa caccggcgca cccaacgttg
     2461 cggtgaccac gccaataggc agctctgcgg cagctaatgc caggcgcgct acaatatcgg
     2521 ccagcagcaa tgcgctcgcc cctgccagcg cgcagccggg aagtaatgcg cgatgatcgg
     2581 ttaaaccaca caaccgaaga atatgtggga tcaccagacc aataaagccg atagcacccg
     2641 ccagcgctac actgacgcca accatccagc cggtcgctgc caccagcaca ttgcgccaga
     2701 accacagggg taaacccagt tgccgcgccg agatctcgcc aagtgctaac atattcatcg
     2761 gcctggactg acaacagatc cacaacaaca tggggatcaa tgccagcatc agccagcttt
     2821 gccgccagtc tacgccgcca aaaccgccca tcatccagta catcagctga cgtaaatcaa
     2881 cagaggtgga aaagtagata gcccacgtca ttagtgcgct acagataatc cctaatgcaa
     2941 cgccagccag caataaccga ctggtcgaaa gatgacgacg ggcgaaacgt aagagtatta
     3001 aagtgatgat aagcgcccca gcaatcgcac acagccctag cgcccagttg gggagttgcc
     3061 cttgcccaag caataccgcg gcgataagcc ccacgcctgc gccgttagag acgccaagta
     3121 gtccaggttc tgccagagga ttttcaaaca acgcctgcat tacagcgccg gatatagcca
     3181 gcgccgcacc aaccagcaat acagccagcg tacgtggcag gcgaatttgc cagacgaaca
     3241 gttcgccacg aggagaaaac cagtcacctg gcgagatcca ttgttcaccg gcgcaaaggc
     3301 ttaagagaag cgccagcagc atcaaaactg acaggcataa taaccagcga atattttgtc
     3361 gctgttgttg gcgggcaagt gtcagcatgg tatccgttct gctgaagtgt catggcgttg
     3421 attttacggt gactcttcga cagtgaaaag aaaaaaggcc gcagagcggc ctttttagtt
     3481 agatcagatt actcgtcttt gggcgaagcg ttttcgaccc ggctttttaa cttctgcccg
     3541 ggtctgaagg tcaccacgcg ccgtgctgta atgggaatat cctcgcccgt tttcgggtta
     3601 cgtcccgggc gttgattctt atcacgcaga tcgaagttac caaaaccaga gagtttcacc
     3661 tgttcgccgt tttccagagc gcgacggatc tcttcgaaaa acagttcaac cagttctttg
     3721 gcatcccgct tgctaagccc aagcttatca aacagatatt ctgacatttc agcttttgta
     3781 agcgccatag gttcaatccc tcaatgatgc ctggaatcgc tcttttaatg cctctacaca
     3841 tttggcgacg gtagcggcaa tctcctcttc ttcgagtgta cggctggtat cttgcaggat
     3901 caggcttatg gcgaggctct tatacccctc cgcaacaccc ttaccgcggt acacgtcaaa
     3961 taagtttacg ccaactacct gatttacgcc aactttctta cattcggata aaatatccgc
     4021 tgcgggaacg ttttctgcga ccaccaccgc gatgtcacga cggttcgccg ggaagcgaga
     4081 aatctcgcgc gcctgaggca ccacgcggtc tgcgagcttg ttccactcca gttcgaacac
     4141 cagagtgcga ccgttaagat ccagtttacg ttccagttca ggatgaacaa ccccaacaaa
     4201 accaatacgt tcacctttca gataaatcgc tgcggattgc cccggatgca gtgccggatt
     4261 cgcttctgca cggaactcaa cctcattcag tttaccggtc aggtcgagaa cggattcaag
     4321 atcgcctttc aaatcataga aatcaacggt ctcttttgcc aggttccagt gctcttcgta
     4381 acggttaccg caaatcacac cggctaacat cagatcctga cgaatgccca acggtgcctg
     4441 agtatctggt acgaaacgca gaccgctttc gaaaatgcgc acacggttct gctgacggtt
     4501 ctggttgtac accacggttg ccagcaggcc ggtccacaga gaaagacgca ttgctgacat
     4561 ttcaacagag atcgggcttg gcagcagtaa ggtttcaacg cctggatgga tcatctgctg
     4621 cactttcgga tcaacgaagc tataggtgat cacttcctga tagcctttat cgttgagcag
     4681 cgttttcacg cgcttgagcg acaggtcagc ttcacggtga gtacccataa tcaggcttgc
     4741 ctgtaccggc tcatccggga tgttgttgta gccgtaaaca cgcgcgactt cttcgaccag
     4801 atcttcttca atctccatgt cgaaacgcca gctcggcgca actgcctgcc actcgtcttt
     4861 gccttcggtc acttcgcagc cgagacgacg cagaatgtca gttacctgct catccgcaat
     4921 atgatggccg atcaggcgat ccagtttgct acgacgtaaa gtgatggttg cacgcttcgg
     4981 cagcgttgct tcgttggtga tatcaattac cggaccagcc tcaccaccgc agatgtcaat
     5041 cagcagacgg gtcgcacgtt ccatcgcttt gtactgcagt gccggatcaa cgccacgctc
     5101 ataacggtga gacgcatcag tatgcaggcc atgacgacga gcacgaccgg tgatagacag
     5161 cgggctaaag aaagcgcatt ccagcagcac gttttgtgtt tcgtcattca cgccagagtg
     5221 ttcgccaccg aagatgccgc ccatcgccag cgccttgttg tggtcggcga tgaccagagt
     5281 gtcagcattc agcttcgctt cagtaccgtc gagcagcacc agcgtttcgc cctctttcgc
     5341 catccgcacc acaatgccgc cttcaatgcg atctttatcg aaagcgtgca tcggctggcc
     5401 caattcgagc agcacatagt tggtgacgtc aacaactgca tcgatagaac ggatcccgca
     5461 acgacgcagt ttttctttca tccacagcgg agttggcgct ttaacgttaa tgccttttac
     5521 cacacggcca agataacgcg ggcaggcttc cggcgcttcg actgtaatcg gcagcgtgtc
     5581 gtcgatggtc gcaccaaccg gaacgatttc cggttcaacc agcggcagct gattcagcac
     5641 ggcaacgtca cgcgcaacac caatgatacc taaacagtcg gcacggtttg gcgttacgct
     5701 gatttcgatg gtgttgtcat cgagtttcag gtattcgcgg atgtcagtgc caatcggcgc
     5761 atctgcaggc agttcgataa tgccgttatg gtcgtcagaa attcccagct cagaaaagga
     5821 gcacagcatc ccttcagacg gttcgccgcg cagtttcgcc gctttaattt tgaaatcacc
     5881 cggcagaaca gcaccaatgg tcgctaccgc cacacgcagg ccctgacggc agtttggcgc
     5941 accgcagacg atgtccagca ggcgatcgcc gccgacattc acttttgtca cacgcagttt
     6001 gtcagcgttc ggatgctgcg cacactcaac cacttcacca acgaccacgc cgtggaagct
     6061 accggcaacc ggttctacac cgtcaacttc caggcccgcc atagtgattt gattcgccag
     6121 cgcatcgcta tcaatcgccg ggttcaccca ttcgcgtaac cacagttcac tgaatttcat
     6181 aatctattcc tgccttattt aaactgtttg aggaaacgca gatcgttttc gaagaatgaa
     6241 cgcaggtcgg tgacgccgta acgcaacata gtcagacgct ccatccccat cccgaaggcg
     6301 aaaccagagt aaacttccgg gtcgatgcca acgttacgca gcacgttcgg atgcaccatc
     6361 ccgcagccca gtacttccag ccatttaccg tttttaccca tgacatccac ttctgcagaa
     6421 ggttcggtaa acgggaagta ggaaggacgg aagcgaatct gcaaatcttc ctcaaagaag
     6481 ttacgcagga agtcgtgcag cgtgcctttc aggttggtaa agctgatgtt ggtatcaaca
     6541 atcagacctt ccatctgatg gaacatcggc gtgtgagtct ggtcgtagtc gttacgataa
     6601 acacggccag gcgcgatgat acgaatcggt ggctgctggg ctttcatggt gcggatctgt
     6661 acgccagagg tctgggtacg cagcaggcgg gtagcgtcaa accagaaagt gtcgtggtca
     6721 gcgcgcgccg ggtggtgacc aggaatgttc agagcatcga agttatgata atcgtcttcg
     6781 atttccggcc cggttgccac ggtaaagcca agctcaccga agaaactttc gatacggtcg
     6841 atggtacggg taaccggatg cagaccgccg ttttcaatgc gacgacctgg cagagagaca
     6901 tcaatcgttt ccgccgccag acgcgcattc agtgcagcgc tttccagttc cgctttacgc
     6961 gcattcagcg cctgctgaac ctgctctttc gcttcgttga taaccgcacc ggctgccgga
     7021 cgctcttctg gcggcagctc acgcagggtc gtcatctgaa gggttaagtg ccctttttta
     7081 cccaaatatt cgacgcgcac attgtctaac gcggcaacat ctgacgcctg gctaatggcc
     7141 gccttcgcac tggcaaccag ttctgcgaga tgtgacatgg ttttcctcat tgtgtcagtg
     7201 gtgacactgg ttcgttggac ttagagccta tcccatcagg ctattttact tgccattttg
     7261 gtcccgggca gtgctcgaaa tcctcacgta cttaagtacg ctccggtttc tccgcactgg
     7321 ccgtatccag actggctgcg acaattacgc ctgatagact ggctttttat ttttcaaaac
     7381 gcgcatacaa aaaaagcctc cactgggagg ctttcaggcg ctgttttccg tttctcttct
     7441 cacgcgctag cctcctggat tcaggtgcta aagtaaaaaa agaagcggaa aatagcagca
     7501 ttcattgctt gcgttacctt ttggtactct tcaaaagacc tttattgaaa aggctacggc
     7561 gataaaagtc aatgttttga tggcgttgaa acgaaaagag ggagactagc tccctctttc
     7621 aactggctta tgccagagct gctttcgctt tttcaaccag agcggtgaac gctactttgt
     7681 cgaatactgc gatatcagcc aggatcttac ggtcgatttc aacagaggct tttttcaggc
     7741 cattgatgaa tttgctgtaa gaaataccgt tctgacgtgc tgctgcgttg atacgcgcaa
     7801 tccacagttg acggaactga cgcttacgtt gacgacggtc acggtaagca tactgaccag
     7861 ctttgataac agcctggaag gcaacgcggt atacgcgaga acgcgcaccg tagtagcctt
     7921 tagcttgttt caaaattttc ttgtgacgtg cgcgtgcaat aacaccacgt tttacgcgag
     7981 ccatatgtgc tctcctgtat ctatattcta attaaaaagt taaaaacgtt aacggcttat
     8041 gcgtacggca ggcacgcgat taccaggccc agatcgcctt tggaaaccat ggctttcgga
     8101 cgcaggtgac gtttacgttt ggtcgctttt ttggtcagaa tgtgacgcag gttagcgtgc
     8161 ttgtgcttaa aaccaccttt accggttttt ttgaagcgct tagcagcacc gcgtacggtc
     8221 ttaatttttg gcattttaat aacttccact tcgcattgtt aataaacgaa acaaaggcga
     8281 acaaagcctg tgaagcccga aggctccaca gacagtgcta cttgaaggcc ttactgtttc
     8341 ttcttaggag cgagcaccat gatcatctgg cggccttcga tcttcgttgg gaaggattcg
     8401 accactgcca gttcttgcaa atcgtctttc acgcgattaa gcacttccat accgatttgc
     8461 tggtgcgcca tctcacgacc gcggaaacgc agcgtgattt tggctttatc accctcttcg
     8521 agaaagcgaa tcaggctgcg gagttttacc tgatagtcgc cttcatctgt accaggacgg
     8581 aatttaattt ccttaacctg gataactttt tgctttttct tctgttcctt agaagacttg
     8641 ctcttttcat agaggaattt gccgtaatcc attatacgac aaaccggcgg ctcggcgtta
     8701 gggctgatct cgactaagtc tactccggct tcttctgctt tctccagagc ttctctcaga
     8761 ctcacaatac caagctgctc gccttccaga cctgttaagc gaacttcctg ggcgcgaatt
     8821 tcgccattga tacggttagg gcgcgccgtt tgaactcgtt ttccgccttt aataccttat
     8881 tcctccaatt gtttaagact gcggctgcga atctcttgtt gcagcttctc gatcacttca
     8941 tttacgtcca tgcttcccag gtctttacca cggcgggtgc gaacggcaat tttgcctgat
     9001 tccacctctt tatcaccaca gaccagcata tacgggacgc gacgcaaagt gtgctcgcgg
     9061 attttaaagc caatcttctc atttctcaag tctgctttaa cacgaatgcc cgcatttgat
     9121 agtttttgcg tcaattcgtt aacgtattca gactgtgaat cggtaatatt catgataaca
     9181 acctgaactg gcgcaagcca ggtcgggaag aaaccagcga attcttcggt caggataccg
     9241 atgaaacgtt ccatcgaccc cagaattgcg cggtgaatca ttaccggtac cttacgctcg
     9301 ttgtcttcgc ccacatagga ggcgctcaga cgagacggca aggagaagtc cagctgtact
     9361 gtaccgcact gccatgcacg atcgaggcag tcatacagga taaattcaat tttcggaccg
     9421 tagaaagcgc cttcacccag ttgatattca aacgggatgt tgttttcttc cagcgcaacc
     9481 gccaggtccg cctcagcacg atcccacatt tcgtcgctgc caatacgctt ttcaggacga
     9541 gtggagagtt tgacgacgat cttctcgaag ccaaaagtgc tgtacatatc atagactaaa
     9601 cggatacatc cgttaacttc atcgcgaatt tgttcttcag tacagaagat atgcgcgtca
     9661 tcctgggtga atccacgcac gcgcatcagg ccatgcagag aacctgacgg ctcgttacgg
     9721 tggcagctac caaactcggc catacgcagc ggcagatcgc gataagactt cagcccctgg
     9781 ttgaaaattt gtacgtgacc cgggcagttc atcggcttaa tgcagtattc acggttctca
     9841 gaagacgtgg tgaacattgc atctttgtag ttgtcccagt gaccggtttt ttcccacagg
     9901 acacggtcca tcatgaacgg acctttaact tcctgatact ggtactcttt cagtttagaa
     9961 cgaacaaaca cttccagttc acggaagatg gtccagccgt cgttgtgcca gaataccata
    10021 cccggcgctt cttcctgcat atggtacagg tcgagctgtt taccgatttt acggtggtcg
    10081 cgtttcgcgg cttcttccag gcgctgcagg taagcgttaa gcgctttttt gtctgcccac
    10141 gccgtaccgt aaatacgttg caacattttg ttgttgctgt cgccacgcca gtaagcccct
    10201 gccgttttca ttagtttgaa atgatggcag aaacgcatgt tcggtacgtg cggaccgcgg
    10261 cacatatcga catattcttc atggaagtac agacctggct tgtcatcatg ggcgatgttt
    10321 tcgtcaagaa tggagacttt gtagctctcc ccacggttgg cgaaagtttc acgcgcttcg
    10381 tgccagctga ctttcttctt aatgacgtcg tagtttttct cagcaagctc atgcatccgc
    10441 ttctcgagtg cttcgacatc ttcctgggtt aacgtgcggt caagatcaac gtcgtaataa
    10501 aaaccgttgt caataaccgg gccgattgcc attttggtat gcggccaaag ttgtttaatc
    10561 gcgtgcccta acaggtgcgc acaggagtga cgaatgatct ccagaccttc ttcgtctttg
    10621 gcggtaatga tcgacagttg tgcgtcgttt tcaatcagat cgcaagcatc aaccagttcg
    10681 ccattaacgc gccctgcgat acaggctttc gccagacctg gaccaatgtc cagcgcaaca
    10741 tccatggggc ttacagcgtg gtcgtaatgg cgttgactgc catcaggaag agttataaca
    10801 ggcattttat atccttattt gcagtggtga cccacacgaa agatcacata caaagaaaaa
    10861 tttgtttatt aacagttcat tgcgaaacca tctagccaac aaatgctaaa ttggttcgca
    10921 atcaggtaca caaatgaaag acattaacct ctcaccttcc acccggttga gcgatgttaa
    10981 cactaaaaaa agggagattg tacctttccg tttcacattg attcgattcg caattcgttt
    11041 gtagtgagta caccagcaaa acgcttaaag aacaactgat aacatcacgg agcgtctgaa
    11101 aggtttactg cgagcgaatt tacagcagca taaactgaat ccagcaaagt tcgttgcgtg
    11161 atttggcggt actatttaaa cgatcataag attaatgacg aagtggtcat atcacaatga
    11221 taaaagtgac acaattctta tagcaatttt tcgtgcacat ttcgttctgg cgataataat
    11281 taatcatcat actcaaaacc tccagacata tataaggcga atagattatg agtcaaaacg
    11341 acattattat cagaactcat tataagtctc ctcatagaat gcacatcgat agcgacatac
    11401 caacgccttc atcagagcct attaatcaat ttgcgcccca actcatcacc ctacttgata
    11461 cctctgactt aagttcgatg ctgtcatact gtgttactca ggaatttacc gcaaactgtc
    11521 gaaaaatatc acaaaattgt tattccactg ccctttttac cattaacttt gccacttcac
    11581 ccatccatgc agaaaatata ttcattacat tacactataa aaaagaaatc atttccttat
    11641 tactggaaac cacgcctatt aaagctaacc atttgcgaag catactggat tatattgaac
    11701 aggaacagtt aactgccgaa aatcgtaacc attgtatgaa actgtctaaa aaaatccata
    11761 gagaaaaaac tatacaacca acagtaaatc tcaatagtag tgcatttttt tcgcaatctc
    11821 cttctgacgc tattttttgt cgccatctgt cattgcaata cgcacttgat tcattgagaa
    11881 atggaaaagg caaggtcaac ctgattaaac attactcctc cgttgaatcc atacagcagc
    11941 atgtcccctt agtccgggac gcggagttca gagcattact tcgccatcct cctgcaggga
    12001 gtcgcgttat cgcgagtaag gattttggct tcgctttaga tattttcttc tgtcgaatga
    12061 tggcaaacaa tgtcagtcat atgtctgcga ttttatatat agacaatcat actttgtcag
    12121 taaggctacg aataaagcag tcggcgtatg ggcaattaaa ttatgttgtg tccgtttacg
    12181 acccgaacga taccaacgtt gccgtcagag gcacccacag gacagcacgg ggctttctct
    12241 cgcttgataa gttcatcagt tcaggtcccg atgctcagac ctgggctgat aggtatgttc
    12301 gcaactgtgc aattgctatt ctgcccctat tacctgaggg agttccaggg gctattttca
    12361 cgggtatcgc gacacgaatg ccatttgccc ctatacatcc atcggcaatg ttgttaataa
    12421 tggccacagg ccagactcaa cagcttatta cattattcag acagttgccc atactccctg
    12481 aaaaagaaat cattgaaata ataactgcgc agaatagcat tggtacacct gctttatttt
    12541 tggctatgat gaacggacat actgacaacg tgaaaatatt tatgcaagaa attcagtcac
    12601 tggtagataa tcacatcatt catgaagata atctggttaa attgttgcaa actaaaagtg
    12661 ctaacgaaac acctggactt tatatctcca tgttgtatgg attcgatgag ataatcgata
    12721 tctttctgaa tgcattaacc actcctatag cacaagagct tttaaacaaa aaactggtga
    12781 tgagtatttt agccatgaaa atacatgatg gtgagccagg attatacgcc gcaatggaaa
    12841 ataatcaccc tttgtgtgtc acacggttcc tctctaaaat taatggcatc gcctttaaat
    12901 acaagttgag caaagctaac atcatggatt tattaaaagg cgctacagca cagggaaccc
    12961 ctgcattata catcgctatg agcaagggta atgaagacgt cgtgttatct tatatatcga
    13021 cgctgggtgc ttttgcaaaa aaacattctt ttagtcaaca tcagttattt acactgttgg
    13081 ctgctaaaaa tcatgacaac atgtcagctg ttcatatagc cattcatc
//
LOCUS       DAZKFZ010000120        13092 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000120 DAZKFZ010000000
VERSION     DAZKFZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            137..646
                     /locus_tag="ACHOQW_003103"
     CDS             137..646
                     /locus_tag="ACHOQW_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP9574145.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="ACHOQW_003104"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="ACHOQW_003104"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP9574146.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHOQW_003105"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHOQW_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP9574147.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHOQW_003106"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHOQW_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP9574148.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHOQW_003107"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHOQW_003107"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP9574149.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHOQW_003108"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHOQW_003108"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP9574150.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHOQW_003109"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHOQW_003109"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP9574151.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7862
                     /gene="nei"
                     /locus_tag="ACHOQW_003110"
     CDS             7071..7862
                     /gene="nei"
                     /locus_tag="ACHOQW_003110"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP9574152.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKSIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7859..8905)
                     /locus_tag="ACHOQW_003111"
     CDS             complement(7859..8905)
                     /locus_tag="ACHOQW_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9574153.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9054..10115)
                     /locus_tag="ACHOQW_003112"
     CDS             complement(9054..10115)
                     /locus_tag="ACHOQW_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9574154.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10112..10843)
                     /locus_tag="ACHOQW_003113"
     CDS             complement(10112..10843)
                     /locus_tag="ACHOQW_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9574155.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10858..>13092)
                     /locus_tag="ACHOQW_003114"
     CDS             complement(10858..>13092)
                     /locus_tag="ACHOQW_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9574156.1"
                     /translation="QKSGDKIIPCINESLIELFGLKSDFRKKLPAIKECVDFSVFPEI
                     IFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFLMDYNLFASTYRPQSGSS
                     SNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAISRTYLFRPLPQIGSRLTL
                     GETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGIAQTNATVTISHSGRVIY
                     QKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAASTPFLTRQGQVRYKLAA
                     GRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDDYRSGALGIGQNMLWMGA
                     LSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLAAYRFSDRHFHSYANYID
                     HKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWNADTSTTANITVGFNVDI
                     GDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLGYDMQNNSNTTTHRMSWN
                     DTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDLTGTYAANDYTSASASWS
                     GSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDYTNRFGIAVVPFVSSYQP
                     TTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGKDLNVIISDANGHFPPLG
                     ADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKTCAIHLPEHLEDVTKRLI
                     LPCH"
BASE COUNT         3144 a         3256 c         3612 g         3080 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcagcc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaagc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaaagacctc aatgcggcgc aactggatgc actcgcacac gcgttactgg
     7681 atattcctcg gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg
     7741 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca
     7801 tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgtccgggc tgccagcact
     7861 aggccgtacg tttcggcgca taggttgaaa taaaccgcgc aatggcaggc cctgtcagca
     7921 aaatactgaa caggcgcagg gtttgcatcg ccataatgag cgccatatcg gcattgctcc
     7981 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcagtca
     8041 taaaatcaat gtgcataaac cgggtcagtc cccacgccat acccgcacaa atagccagca
     8101 gagcaaaaat cgacagcagg atttgtggta gcgggcgtag tgcccgcagt aagatttgtt
     8161 tatcgaaacc aaggccaatc cgccagccaa ttgccatata cgccatcgcc agtagccatt
     8221 ccggtagttc gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8281 tgagcatcgt gccggaagga agacgcagca ggcatcccac tgtaccggca acgaccgcga
     8341 gcaaaatagt aagcaggaga ttaatgctca ctggcgggaa ccagacgata tgctggttaa
     8401 ccgcttcagc gttatcgccc agcatcattc gcgtgaccag aactgcggcc cccgcgacaa
     8461 acagcactcg cagatactgc ataaacgcca ccaggcggat atctgcgccg taatcctgcg
     8521 ccattgcgac cattgccgcc gccccgccag gcgaggagcc ccaggcacca gtatttccgg
     8581 gcagtgagct atagcgcacc agtagccatc ccacgatagc gctggaaagc aacgtcacca
     8641 gtaaaatagc taacacaatc ggccagttaa cctccagcgt ggtcaggatc gaaccggtca
     8701 agttttgcgc aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggga
     8761 gttgcagggt aaccccgcgc atactgaaga tgatcccggc gatcatcggt ccaagtaaga
     8821 gtgccgccgg gagatgtaat ccgaggaaac caatagaaag gagaagtgat agcacacata
     8881 acattcccca ctgcaaaact ggcatccttt gctccctcat tattttctct ttggtgtatc
     8941 gatacagcat aaagaatgcc gggcatggaa aaaataacgg taatcaataa aagccggaaa
     9001 attggcaatt aaccgaagaa gccctctccg gctggaaagg gcaataggct aaatcagttg
     9061 tagttaattt tgaaaagtac cactgtatca aagggaccaa cgtcgatggg cgtacccggc
     9121 gtggatgaca attctgccgt gtaggttttt tcatacacta acaggtcgct ggtaaaatcg
     9181 gcatactcac tgtatttgtt gaaagtatag ggtgaggcat tttcatccaa caatcgaagc
     9241 ttcaagccat tgccaatgag cagtgattta tcttcgtcga taagggtttc atcggtataa
     9301 aacgaagagc tgactttaaa accgtcggta cactggtcgt tttgtgattt ggttgtagtt
     9361 attttaaatt gctgcgacat cgtatggcga cgaatatcca gcacgttaaa tcggccaaaa
     9421 tcaacgacct gggtttcagg tgagatagca aagttgacgc tgcaatccag cacgcggata
     9481 ttttccagac cggtaatgtg atatttcaga ttcttcgcgg tagggtcttc gttcacgctg
     9541 cctgcgccat caaactgtac gacgatgtaa tcgctgagtt tgctctgata atcatggggc
     9601 ggcatggcgc gaactttaac gtaaagccgc atacgcgcca caaacgtccg cgatttgtgc
     9661 ataacggttg gatcgccgga gcaaagttta ttttgccagc ccatcgcaat gatctgctcc
     9721 ggggtgtaaa tatcaatatt cttactgtcg atgcactgat tcgtgtcgat gcggcttttc
     9781 ccaaggcttg cgtcatagtc cacgccttca taggttacgc ccaattgata atagggatcc
     9841 tgaataccgg ggtaagggtt tacccaggcg tagacgtgct cgctttcaaa attaccgttt
     9901 gtgttgttgt cacagtagac cggaattttt atatcgtcag attcccagat tttatcgccg
     9961 ggtttggaat tggccggaac ggcgaagggc ataatagctt ccgatttttc tacagcgccg
    10021 tttgcggtac caaaatagca gttcagcgcc agcgccgctt ttagcggcaa aaaaatcaga
    10081 cagatggcga gcaacacgcc tttggcgaat ctcatttttt attctccacc ggctgcaggc
    10141 ggcattgccg ctggcaggag agcgtgttca tacgcaatgc gccgtagtcg tccatgtagc
    10201 caagataaaa ctgcttgccg ctatagctgc ccgtgctggt ggtgaccgaa ctgaacggtg
    10261 cgaccatcgt gcttttaaag ccaggcagca cgcctttctc atcccgccca agataggcaa
    10321 tagtcagata gtatccggtc ggggttttca gggtaagctg gttattctgc tggctaacct
    10381 gtagctgttg ctcaacatga tcgcccattt ttttgcgtaa tgcggcgggt cgccagaata
    10441 gcttaatgcg actttggacg gcgacttgca ggatcgcatg gccctcgctg ccttccggga
    10501 caggcggaat ttcacgcagg ttatagtaaa agagagattc gcgatcggtg ggtaatttcg
    10561 cggtggcggc ttgtttcatg atccgaacct gcgaagtcgc ttttggctcc agtcgctgga
    10621 taggcggcag ggcgacgaga aattcatcgc ttttctctcc cttttcattt tctatccatg
    10681 aataggcgag gtaggggagt ttatcgctgc ggttttccac gcgcaggctg gttgctttat
    10741 ctttgctatt aaagatgatg cgcgtgcggt caggctgcac tgcgccgtgg ctgcacatac
    10801 tggcaaccag aagaatcaaa gggaatcctt taacaaatgt catcgtcttt tccttaatta
    10861 atgacagggt aaaatcaggc gcttagtcac atcttccaga tgttccggca gatgaatggc
    10921 acacgttttc tggtcgcccc agtgcacggt aaattgttga ttttcatcga ccccactcag
    10981 ccatgcgtga ccgttttcgc caaccatccc gacgctgaca ccgccttcag cctggcgaac
    11041 atcggcaccg agcggcggga aatgcccgtt agcatcgctg ataatgacgt tgagatcttt
    11101 cccggcgcgg gacgccagcg atttaaagcc gatggcgcct tcggtccagg tttctttcac
    11161 cacgttttcg gaaacggtaa cgccgtcagg caagtcgttc atattgaccg ccaccgtcgt
    11221 aggctggtag ctggaaacaa acggtaccac ggcaatacca aagcggttgg tgtagtcaat
    11281 attgccctga atcggaatat cgcccacgcc gtcggtgctg accatcaggc gtggttcatt
    11341 ggtggaactg cggcgatgga atgccgcgcc atgttgagtc gcggtaaacg agccgctcca
    11401 actggcactg gcggaagtgt aatcattggc ggcataggtt ccggtcagat cccattcccc
    11461 cgccgaactg aggtgttggt aattgccgct gacctgcgca ccgttatccg ggcgatcgga
    11521 ttgtattcca gccgacattc cccaactgtt tcgttcatcc agggtatcgt tccacgacat
    11581 gcggtgggtg gtagtattac tgttgttctg catgtcataa cccagtcgac cactttcccc
    11641 aatcggtaag gagatcgaaa agtaaatctg gttgtcgcga tctttatctt cataatgggt
    11701 ggtgttgaac gaggtcgaga cagaaatatc tttccagtcg ccaatgtcga cgttaaaacc
    11761 gacggtaatg ttggccgtgg tggaggtatc cgcattccac cagctctgat gcaggatgtt
    11821 ggcgtagaga ttaagattca gcagagtaat aggctgaccg aaggagaggc tgattgtttg
    11881 cttttcatcc tgcgcgtcgg cgtcattgta tttgtgatca atgtagttag cgtagctgtg
    11941 aaaatggcga tcggagaagc gataggctgc caatgagatg gtgctgttgg tcgcatcaac
    12001 ctgtttgctg tagttaaagc ggtagctgta accctgctca tcctgttggg tatcaaaatg
    12061 gctgtcggcc caggtcacgt caaacgacag cgcccccatc cagagcatat tttgcccaat
    12121 acccagcgcg ccggaacgat agtcatcgcc tgcgagaagc atgccgccat atagtgaagt
    12181 gttggagagc attccccagg agacttcatg gctaatgaag gtttcatcct cggtgtggtg
    12241 cgacatagag gaacgcgggc gaccggcagc cagtttatag cgcacctggc cctgacgcgt
    12301 caggaacggg gtagaggccg ctgaaacctg gaagttattc acccgtccat cttcttcgct
    12361 cactttgaca tccagtgtac cttgcactga ctgattcagg tcatcaataa taaatgggcc
    12421 tggtggaact tttttctggt aaatcacgcg accagaatgg cttatcgtga ccgtggcgtt
    12481 agtctgtgcg atgccgctga tttgcggcgc gtagccgcgt aactcccagg ggagcattcg
    12541 atcgtcgctg gctaacgccg caccggtata ggagaaacca tcaaaaatgt tagagctaaa
    12601 atcggtttcg cccagggtta atcgggaacc tatttgcggt aacgggcgga acaggtaggt
    12661 ccgtgaaatc gccccagatt gttcacggtt gtcgccgcta tcggactggc taagctgata
    12721 atcgctgcgc agacgccatg ccccggcgtt gaggcccgtg gtgccatagg cgttcaggtt
    12781 gttgctgctg ctgccgcttt gtgggcgata agtgctggca aacaggttgt aatccatcag
    12841 gaagccagga ataccgttat tccacgttga gggtggcgtc cagttttctg aatgccacgc
    12901 cagccatgcc tgcgggattg taatattgag ctgttgattt gcctgatcga aagtaaagat
    12961 gatttcaggg aagacactaa aatcgacaca ttcttttatt gccggtaatt ttttacgaaa
    13021 gtcagattta agtccgaaga gttcgatcag cgattcattg atacaaggaa taattttatc
    13081 gccagatttt tg
//
LOCUS       DAZKFZ010000121        13091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000121 DAZKFZ010000000
VERSION     DAZKFZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            180..893
                     /gene="aphA"
                     /locus_tag="ACHOQW_003115"
     CDS             180..893
                     /gene="aphA"
                     /locus_tag="ACHOQW_003115"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP9574157.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1004..1420
                     /locus_tag="ACHOQW_003116"
     CDS             1004..1420
                     /locus_tag="ACHOQW_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP9574158.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1424..1780
                     /locus_tag="ACHOQW_003117"
     CDS             1424..1780
                     /locus_tag="ACHOQW_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9574159.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1815..4637)
                     /gene="uvrA"
                     /locus_tag="ACHOQW_003118"
     CDS             complement(1815..4637)
                     /gene="uvrA"
                     /locus_tag="ACHOQW_003118"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9574160.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4892..5428
                     /gene="ssb1"
                     /locus_tag="ACHOQW_003119"
     CDS             4892..5428
                     /gene="ssb1"
                     /locus_tag="ACHOQW_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP9574161.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5527..5808)
                     /locus_tag="ACHOQW_003120"
     CDS             complement(5527..5808)
                     /locus_tag="ACHOQW_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP9574162.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6237..7823
                     /gene="pdeC"
                     /locus_tag="ACHOQW_003121"
     CDS             6237..7823
                     /gene="pdeC"
                     /locus_tag="ACHOQW_003121"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP9574163.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7826..8149)
                     /gene="soxS"
                     /locus_tag="ACHOQW_003122"
     CDS             complement(7826..8149)
                     /gene="soxS"
                     /locus_tag="ACHOQW_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP9574164.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8235..8699
                     /gene="soxR"
                     /locus_tag="ACHOQW_003123"
     CDS             8235..8699
                     /gene="soxR"
                     /locus_tag="ACHOQW_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP9574165.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9245..10594
                     /gene="ghxP"
                     /locus_tag="ACHOQW_003124"
                     /gene_synonym="yjcD"
     CDS             9245..10594
                     /gene="ghxP"
                     /locus_tag="ACHOQW_003124"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP9574166.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10745..12394
                     /locus_tag="ACHOQW_003125"
     CDS             10745..12394
                     /locus_tag="ACHOQW_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9574167.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12548..>13091)
                     /gene="espX5"
                     /locus_tag="ACHOQW_003126"
     CDS             complement(12548..>13091)
                     /gene="espX5"
                     /locus_tag="ACHOQW_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFP9574168.1"
                     /translation="MAYMAEVNLYKVIAPYINLFRADLSFSKLDLINFKHADLSRVNL
                     NKAILQNINLIDSKLFFTRLTNTFLEMVICTDSNIANVNFNNANLNNCHFNCSVLTKA
                     WMFNTRLYRVNFDEASVQGMGISILRGEENIPINSDTLVTLQKFFEEDCTSHTGMSQT
                     ENNTHEVAMKITADIMQHAD"
BASE COUNT         3056 a         3208 c         3432 g         3395 t
ORIGIN      
        1 attttttaca aaaaaacgac aggaacgtat ttactaggtt aaatataatc atcctgcttt
       61 tcatcacaaa aaccgcagat aatccttcct ttcccctgta gctggcgtta tggtcagata
      121 gtttttgcaa caaatctcac aataaaaagt ttcaacatac tgactattta gggaaaaata
      181 tgcgcaagat cacacaggca atcagtgccg tttgcttatt gttcgctcta aacagttccg
      241 ctgttgccct ggcctcatct ccttcaccgc ttaaccctgg gactaacgtt gccaggcttg
      301 ctgaacaggc accaattcat tgggtttcgg tcgcacaaat tgaaaatagc ctcgcagggc
      361 gtccgccaat ggcggtgggg tttgatatcg atgacacggt acttttttcc agtccgggct
      421 tctggcgcgg caaaaaaaac ttctcgccag aaagcgaaga ttatctgaaa aaccctgtat
      481 tctgggaaaa aatgaacaac ggctgggatg aattcagcat tccaaaagag gtcgctcgcc
      541 agctgattga tatgcatgta cgccgtggtg acgcgatctt ctttgtgact ggtcgtagcc
      601 cgacgaaaac agaaacggtt tcaaaaacgc tggcggataa ttttcatatt cctgccacca
      661 gcatgaatcc ggtgatcttt gcgggcgata aaccagggca aaatacaaaa tcgcaatggc
      721 tgcaggataa aaatatccga attttttatg gcgattccga taacgatatt accgccgcac
      781 gcgatgtcgg cgctcgtggt atccgcattc tgcgtgcctc caactctacc tacaaaccct
      841 tgccacaagc gggtgcgttt ggtgaagagg tgatcgtcaa ttcagaatac tgacagagcg
      901 ggagagcgtg acactctccc gaatgctgtt tttttaatca cacctttatc ctttcgctgt
      961 cttgctgcaa actgattaag agagttttat caaggagcag cacatgtggt atcaaaagac
     1021 gctcacgctt agcgccaaat ctcgtgggtt tcatctggta acggatgaaa ttctgaatca
     1081 gctggctgat atgccgcgcg ttaacatcgg cttactgcat ctgttgctgc aacatacctc
     1141 cgcctctctg acacttaatg agaactgcga tcccaccgta cgccacgaca tggagcgttt
     1201 tttcctccgc accgttcccg acaacggaaa ttatgagcat gactatgagg gagcagacga
     1261 tatgccttct catatcaaat cctcaatgct gggaacatcg cttgtattgc cggtgcataa
     1321 agggcgtatt cagaccggca cctggcaagg catttggctg ggggaacatc gcatccacgg
     1381 cggatcgcgt cgcatcatcg cgacactaca aggggagtaa aaaatgacca tttcggagtt
     1441 gctacaatat tgcatggcaa aaccaggcgc agaacagagc gtgcataacg actggaaagc
     1501 gacgcagatc aaagtggaag atgtactgtt tgcgatggtg aaagaagtag aaaatcgccc
     1561 agctgtttcg ctgaaaacca gcccggagct ggcggagctg ctacgtcagc agcacagcga
     1621 tgtgcgtcca agccgccatc tgaataaagc gcactggagc accgtgtatc tcgacggttc
     1681 gctgccggat tcgcaaatct attatctggt ggatgcgtct tatcagcagg cggtgaattt
     1741 actgccggaa gaaaaacgta aattgctggt gcaactctga aaggaaaagg ccgctcggaa
     1801 ggcggcctta acgattacag catcggcttg aggaagcgtg ccgtatgcga agcttcgcac
     1861 tccgcgacgg tttctggcgt accggagacg aggatctcgc cgccgccact gccgccttcc
     1921 ggccccaggt cgacaatcca gtcagcggtt ttaatcacgt cgagattgtg ctcaatcacc
     1981 acaatggtgt tgccctgatc gcgcagttta tgcagtacgt cgagcagttg ctgaatatcg
     2041 gcgaagtgca gaccggtggt cggctcgtcg agaatataca gcgtctgccc ggtgccgcgt
     2101 tttgacagct cacgcgccag cttcacgcgc tgggcttcac caccagaaag tgtggttgcg
     2161 gactgcccca agcgaatgta cgtcagacca acgtccatca acgtttgcag cttacgcgcc
     2221 agagctggca ccgcatcaaa gaactcacgc gcctcttcga tggtcatatc cagcacttcg
     2281 tggatggttt tgcctttgta cttaatctcc agcgtttcac ggttatagcg tttacctttg
     2341 cactggtcgc acggcacgta aatgtccggc aggaagtgca tctccacttt gatcacgcca
     2401 tcgccctgac aggcctcgca gcgtccgcca cggacgttaa agctgaaacg tcctggcgta
     2461 taaccacgcg cacgggattc cggtacgccc gcaaacagtt cgcgcacagg cgtaaacacg
     2521 ccggtatagg tcgccgggtt agaacgcgga gtacgaccaa ttgggctttg gtcgatatcg
     2581 attactttgt cgaaatgctc cagtccctga atatcgcgat acggtgccgg ttcggcgatg
     2641 gtcgcaccat tcaactggcg ttgggcaatc gggaacagtg tgtcgttaat cagcgtcgat
     2701 ttaccggaac ctgaaacccc tgtgatgcag gtaaacagac cgactggcag cgttagcgtc
     2761 acgtctttca ggttgttgcc gcgtgcgcca gtcagcttaa gtactttttc cggattcgcc
     2821 ggaacgcgtt tcttcggcac ttcaatcttg cgtttaccgc tcatgtactg tccggtcaac
     2881 gactccggca ccgccataat cgcttccagc ggaccttccg cgaccacttc accgccatgt
     2941 acacctgcac caggaccgat atcgatcaca tgatcagcgg cgcgaatcgc gtcttcgtcg
     3001 tgctccacca caatcacggt attaccgaga tcgcgcagat ggataagcgt acccaacagg
     3061 cgctcgttat cgcgctggtg caggccgata gacggctcat ccagcacgta cataacgcca
     3121 accaggcccg caccaatctg gctcgccaga cggatacgct gggcttcacc gccggaaagc
     3181 gtttctgccg agcgggaaag cgtcaggtaa ttcaggccga cgttaacgag gaatttcaaa
     3241 cgatcgccga tctctttaag aattttttcc gcaatcttcg cccgctgacc tgcgagtttg
     3301 agattgttga agaattccat cgcatgacca atgctcatgt cggagatggc gggcagcggc
     3361 gtattctcga catacacgtg gcgcgcttcc cgacgcagac gcgtcccttc gcagctggcg
     3421 cacgggcgat tgctgatgaa cttggctaat tcttcacgta ccgcactgga ttctgtctct
     3481 ttataacggc gctccatatt gtgcagcacg ccttcgaacg gatgacgacg gatggaggta
     3541 tcgccacgat cgttcatgta tttgaattca atgttttctt tgccagaacc gtacaacacc
     3601 actttatgca cgttcgcgct caggctgccc cacggcgctt cgacgtcgaa cttatagtga
     3661 tctgccagcg atttcagcat ctggaaataa tagaagttgc ggcgatccca gccacggatc
     3721 gcaccacctg ccagcgacag ttccggattc tggatcacgc ggtcaggatc gaaatattgc
     3781 tgtacgccaa ggccgtcgca ggtcgggcag gcacctgccg ggttgttaaa cgaaaacagt
     3841 cgcggctcca gttcacgcat actgtagccg caaattgggc aggcgaagtt ggcggaaaac
     3901 agcagctctt ccgctttcgg gtcgtccata tccgccacta ccgcggtacc accggaaagc
     3961 tccagcgcgg tttcaaacga ctcggcaaga cgttgggtaa gatcgtcacg caccttgaag
     4021 cgatcaacca ccacttcaat agtgtgtttc ttttgcagtt ccagtttcgg cggatcggaa
     4081 agatcgcaga cttcgccatc aatacgagca cggatgtaac cctggcttgc caggttctcc
     4141 agcgttttgg tgtgttcgcc tttgcgctct ttaatgattg gcgcgagcag catcagacgc
     4201 ttgccttccg gctgcgacag cacgttatcc accatctggc tgacggtttg cgccgccagc
     4261 gggacgtcgt ggtccggaca gcgcggttcg ccgacgcggg cgtacaacaa acgcaaatag
     4321 tcgtggattt cggtgattgt ccccaccgta gaacgcgggt tatgagacgt cgatttctgc
     4381 tcaattgaga tggcaggaga aagcccctca atatgatcga cgtccggctt ttccatcagt
     4441 gacagaaact gccgcgcgta ggcggaaagg gattcaacgt aacggcgctg cccttcggca
     4501 tataaggtgt cgaaagcgag cgaggatttg ccagaacccg aaagcccggt cacgacaatg
     4561 agcttgtcgc gggggataac gaggttgatg tttttgagat tatgggtgcg ggcgccccga
     4621 acttcgatct tatccattca cctttcccgg attaaacgct tttttgcccg gtggcatggt
     4681 gctaccggcg atcacaaacg gttaattatg acacaaattg acctgaatga atatacagta
     4741 ttggaatgca ttacccggag tgttgtgtaa caatgtctgg ccaggtttgt ttcccggaac
     4801 cgaggtcaca acatagtaaa agcgctattg gtaatggtac aatcgcgcgt ttacacttat
     4861 tcagaacgat tttttttcag gagacacgaa catggccagc agaggcgtaa acaaggttat
     4921 tctcgttggt aatctgggtc aggacccgga agtacgctac atgccaaatg gtggcgcagt
     4981 tgccaacatt acgctggcta cttccgaatc ctggcgtgat aaagcgaccg gcgagatgaa
     5041 agagcagact gaatggcacc gcgttgtgct gttcggcaaa ctggcagaag tggccagcga
     5101 atatctgcgt aaaggttctc aggtttatat cgaaggtcag ctgcgtaccc gtaaatggac
     5161 cgatcaatcc ggtcaggatc gctacaccac agaagtcgtg gtgaacgttg gcggcaccat
     5221 gcagatgctg ggtggtcgtc agggtggtgg cgctcctgca ggtggcaata tcggtggtgg
     5281 tcagccgcag ggcggttggg gtcagcctca gcagccgcag ggtggcaatc agttcagcgg
     5341 cggcgcgcag tctcgcccgc agcagtccgc tccggcagcg ccgtctaacg agccgccgat
     5401 ggactttgat gacgatattc cgttctgatt tgtcattaaa acaataggtt atattgtttt
     5461 aaggtggatg attaaagcat ctgccagcca taaaaaagaa gcctccgtta tggaggcttc
     5521 tacgtatcag gtcaaaatca ttggccactg tggcggtgtc tgattcatct cttctatcat
     5581 caccgactcg atgttgtgcc agatggcggt gatgtccgcc attgtgatgc caagcaaccc
     5641 cattgcgaac cagcaggcgg aaaccacgcc cagaccgctg ctgatcactg caagcccgac
     5701 ataatcagac cgacgaaaac ggatgttgag cgtgctggcc agaaacatca ttacggcact
     5761 aagaagttgc cagcgaagaa gaaccacgcc agtggtgagg gtagccatca aacaattcct
     5821 ctgaaaagag ccgatgccct ggacagcgcg gcgttgttca cgggaggcgt ggtacactct
     5881 ggctatcgcg gggcttgcag aacacaaaaa tgaaacactc tgtttgtttc attaattttg
     5941 tgaactatat cacaattgat tgtttgttag ccagattagg tcgtgacttt tattgctgta
     6001 cagattatgt ggtttttcag tggcattaag ggtatatctt cccgccgcct ctgtattcct
     6061 gtagaaaatt aattttgaat atcaatgaat tattttcatc caagtgacga ttaaaaaggt
     6121 atcaatttca aatcaggcaa aagtgctatt tataccgtaa gatttatcta aagacgtcgg
     6181 tagccagggt ttcaccttgc aatggccggg tataaacagg caggaaattg atagcaatga
     6241 atcatcgtgc acgacaccaa ttactggcgt tgccgggcat tatcttttta gttctctttc
     6301 ccatcattct ttcgctatgg attgccttcc tttgggcaaa atcagaagtg aataatcagc
     6361 tccgaacctt tgctcaactg gcgttggata aatccgagct ggtcattcgc caggcagatt
     6421 tagtgagcga tgcagctgaa cgctatcagg ggcaagtttg cactccagcc catcaaaagc
     6481 gaatgttgaa tattattcgt ggctatcttt atattaatga attgatctat gcccgtgata
     6541 atcatttttt atgctcatcg ctgatagtgc ctgtaaacgg ctatacgatt gtatcggccg
     6601 attataagcg tgaacctaat gtttctatct attattaccg cgatacgcct tttttctctg
     6661 gctataaaat gacctatatg cagcggggaa attatgtggc ggttatcaac cctctcttct
     6721 ggagtgaagt gatgtctgat gacccgacat tgcaatgggg tgtgtatgat acggtgacga
     6781 aaaccttttt ctcgttaagc aaagaggcct cagcagcaac gttttctccg ctgattcatt
     6841 tgaaggattt aaccgtacca agaaatggct atttatatgc gacagtttat tcgacaaaac
     6901 gcccaattgc agccattgtt gcgacttcat atcaacgtct tatagcccat ttttataatc
     6961 atcttatttt tgcgttgccc gccggtattt tggggagtct tgttctgcta ttactctggc
     7021 tacgtattcg acaaaactat ttgtctccca aacgcaaatt gcaacgcgcc ctcgaaaaac
     7081 atcagctatg cctttattac cagccaataa ttgatatcaa aacagaaaaa tgtatcggcg
     7141 ctgaagcgtt gttacgttgg cctggtgagc aggggcaagt aatgaatccg gcagagttta
     7201 ttcctctggc agaaaaggag gggatgatag aacaggtaac tgattatgtt attgataatg
     7261 tcttccgcga tctgggcgcg taccttgcaa cacatgctga tcgctatgtt tctattaatc
     7321 tgtcggcctc cgattttcat acgtcacggt tgatagcgcg aatcaatcag aaaacagagc
     7381 aatacgcggt gcgtccgcag caaattaaat ttgaagtgac tgagcatgca tttcttgatg
     7441 tcgacaaaat gacgccgatt attctggctt tccgccaggc tggttacgaa gtggcaattg
     7501 atgattttgg tattggctac tctaacttgc ataaccttaa atcattgaat gtcgatattt
     7561 tgaaaatcga caaatcgttt gttgagacgc tgaccaccca taaaaccagt catttgattg
     7621 ctgaacatat catcgagctg gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg
     7681 aaactgagga gcaggttaac tggctgcgca aacgcggcgt gcgctattgc cagggatggt
     7741 tctttgcgaa ggcgatgccg ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc
     7801 gggagttaac gcgcgggcaa taaaactaca ggcggtggcg ataatcgctg ggagtgcgat
     7861 caaactgccg acggaaaacg cgggagaagg tctgctgaga gacataaccc aggtccattg
     7921 cgatatcaaa aatcggacgc tcggtggtgc gcaactcaac ggcggccagt aacagacggc
     7981 gttgacgaat gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatc cgttgcaggt
     8041 accactttga atagcctgat ttttttgcga cgacatcaat gttaagcggc tggtcaatat
     8101 gctcgtcaat ccatgcgata agatcctgaa taattttctg atgggacata aatctgcctc
     8161 ttttcagtgt tcagttcgtt aattcatctg ttggggagta taattcctca agttaacttg
     8221 aggtaaagcg atttatggaa aagaaattac cccgcattaa agcgctgcta acccccggcg
     8281 aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa agcaaagggt
     8341 tgattaccag tatccgtaac agcggcaatc agcggcgtta taaacgtgat gtgttgcgat
     8401 atgtcgcaat tatcaaaatt gctcagcgta ttggcattcc tctcgcgacc attggtgaag
     8461 cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa cagctttcgt
     8521 cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg cgtgacgaac
     8581 tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg
     8641 gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa
     8701 gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta tctcgctggt
     8761 acacaggagg gtttcccccg acgtcaacac acctcattcg agcacgtggt ggaggttccg
     8821 gttggtgttg atgctttaat tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac
     8881 tttttagccg caatattttt gtcatctcag acgatttttt atcgcaatcc tcaacggtat
     8941 acggctcgat aacgctgcaa tcttgcgcat cgacgataac gtttgcgcat caattgcctg
     9001 gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt gaaaaagccg
     9061 ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc gcggagctaa
     9121 acgtttgctt ttttgcgacg cagcaaattg tcgcaaacct ggagcaggaa gataacgttt
     9181 cgctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca ggggatgttc
     9241 tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt ttaaaatttc
     9301 acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt ttctggcgat
     9361 ggtctactcg gttatcgtcg ttccaggcat gttgggtaaa gcgggcttcc cgcctgcggc
     9421 agttttcgtt gcgacctgtc tggttgccgg actcggttct atcgtgatgg gtctgtgggc
     9481 taatctgccg ttggcgattg gttgcgccat ctccctgacg gcgtttaccg cattcagcct
     9541 ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc tgatgggtgt
     9601 gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca acttgccgca
     9661 cggtgtggcg cacggcacgg ggatcggtat cggtctgttc ctgctgctca ttgccgctaa
     9721 cggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc tgggtgattt
     9781 cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg gcctggaaaa
     9841 actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg tcggtttgat
     9901 cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga gcgatgaaaa
     9961 cggcaattca ctgattggca gcctggacat tatgggcgcg ctgaatcctg tagtcctgcc
    10021 aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta tccgcgctgt
    10081 cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggcg ggaaagcact
    10141 gaccactgac tccatgagca gcgttttctc tggcctggtg ggtgcagctc cggcagcggt
    10201 atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga cggctatcac
    10261 cgttggcgtg ctgttcctgc tgattctgtt cctctctccg ctttcttacc tcgttccggg
    10321 gtatgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca acgtggcgaa
    10381 aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggcgg tattcatcgt
    10441 gctgacctgt aacatcgtaa caggcatcat gatcggcttc gcgactctgg tgattggtcg
    10501 tctggtttcc ggtgaatggc gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct
    10561 ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct gaaaacgggt
    10621 ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc gatattctga
    10681 aaaaatgaga attcaggcat aacgtctttc tcagatcata aaaaacatcg caaacaggga
    10741 acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcgc tgtccggggt
    10801 ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg ccatcggtgc
    10861 gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac tctttttagt
    10921 cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc gtgaatttct
    10981 cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca ccgtggtcgg
    11041 cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg cctttgcgct
    11101 ggcggcggtg ctttctccga ccgatgctgt ggcgctctcc gggattgttg gcgaagggcg
    11161 catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg acgcctccgg
    11221 cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct tcaccgtcgg
    11281 cggtgcgacg gtcgaattta tgaaagtagc cattggcggt attctcgccg gttttgtggt
    11341 gagctggctg tacggtcgct cgctgcgatt cctcagccgc tggggcggtg atgaacccgc
    11401 gacgcagatc gtcctgctgt tcctgctgcc attcgcttct tatctgattg ccgaacatat
    11461 tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc gctccggtgt
    11521 gatgcgccgt gcgccgctgg caatgcgcct gcgtgcaaac agcacctggg cgatgctgga
    11581 atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg gtattctgga
    11641 gacgtcgctg atggcagcag aaatcgaccc taacgtcgaa atctggatgc tgtttaccga
    11701 tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt ggacgatgaa
    11761 aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt ggaccacacg
    11821 agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactttgg ccggtgtgct
    11881 ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg agctggtgtt
    11941 tctggcggct ggtgtcattc tcttctcgct gtttgtcggc gtggtgatgt tgcctattct
    12001 gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac gtattgcgcg
    12061 agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc gtctggcggc
    12121 ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc gcgtcattgg
    12181 taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc aggaagagaa
    12241 ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac tgtaccacct
    12301 gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg atctcgattt
    12361 gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa ccaggcccga
    12421 ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg gctaacattc
    12481 atgattctaa aacaaataag ttatctctct tttaaagtca tttcattggc tgttcttcga
    12541 cttaatctca atctgcgtgt tgcataatat ctgcagtaat cttcatagct acttcatgag
    12601 tattattctc agtttgtgac atgccagtat gagaggtaca atcttcttca aagaatttct
    12661 gtagtgttac cagggtatca ctattaatgg gaatattctc ctccccacgg agaatggaaa
    12721 tgcccattcc ctggacgcta gcctcatcga aattaacccg atagagacgg gtattaaaca
    12781 tccaggcttt tgttaaaaca gaacagttga aatggcaatt gtttaaattg gcattattaa
    12841 aattaacatt agctatatta gaatcggtgc atataaccat ttcgaggaac gtatttgtta
    12901 gccgtgtaaa aaagagttta ctatcaatta agtttatatt ctggaggatt gctttgttca
    12961 gattgacgcg agacagatca gcatgtttaa agttaattaa atcaagttta gagaagctaa
    13021 ggtcggctct aaataaatta atatatggcg ctattacttt ataaagattt acctcagcca
    13081 tgtatgccat a
//
LOCUS       DAZKFZ010000122        13001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000122 DAZKFZ010000000
VERSION     DAZKFZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            92..883
                     /gene="nanR"
                     /locus_tag="ACHOQW_003127"
     CDS             92..883
                     /gene="nanR"
                     /locus_tag="ACHOQW_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP9574169.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            1005..1898
                     /gene="nanA"
                     /locus_tag="ACHOQW_003128"
     CDS             1005..1898
                     /gene="nanA"
                     /locus_tag="ACHOQW_003128"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9574170.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            2007..3497
                     /gene="nanT"
                     /locus_tag="ACHOQW_003129"
     CDS             2007..3497
                     /gene="nanT"
                     /locus_tag="ACHOQW_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP9574171.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            3545..4234
                     /gene="nanE"
                     /locus_tag="ACHOQW_003130"
     CDS             3545..4234
                     /gene="nanE"
                     /locus_tag="ACHOQW_003130"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9574172.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            4231..5106
                     /gene="nanK"
                     /locus_tag="ACHOQW_003131"
     CDS             4231..5106
                     /gene="nanK"
                     /locus_tag="ACHOQW_003131"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9574173.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            5103..5567
                     /gene="nanQ"
                     /locus_tag="ACHOQW_003132"
     CDS             5103..5567
                     /gene="nanQ"
                     /locus_tag="ACHOQW_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP9574174.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(5627..6674)
                     /gene="yhcG"
                     /locus_tag="ACHOQW_003133"
                     /pseudo
     CDS             complement(5627..6674)
                     /gene="yhcG"
                     /locus_tag="ACHOQW_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(6858..8276)
                     /gene="gltD"
                     /locus_tag="ACHOQW_003134"
     CDS             complement(6858..8276)
                     /gene="gltD"
                     /locus_tag="ACHOQW_003134"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP9574175.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(8289..12749)
                     /gene="gltB"
                     /locus_tag="ACHOQW_003135"
     CDS             complement(8289..12749)
                     /gene="gltB"
                     /locus_tag="ACHOQW_003135"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP9574176.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
BASE COUNT         2817 a         3518 c         3584 g         3082 t
ORIGIN      
        1 gaggcacaaa gagaaaaccc tgccattttc ccctactttc aatcctgtga taggatgtca
       61 ctgatgatgt taatcacact gaccttacag aatgggcctt atgaacgcat ttgattcgca
      121 aaccgaagat tcttcacctg caattggtcg caacttgcgt agccgcccgc tggcgcgtaa
      181 aaaactctcc gaaatggtgg aagaagagct ggaacagatg atccgccgtc gtgaatttgg
      241 cgaaggtgaa caattaccgt ctgaacgcga actgatggcg ttctttaacg tcgggcgtcc
      301 ttcggtgcgt gaagcgctgg cagcgttaaa acgcaaaggt ctggtgcaaa taaacaacgg
      361 cgaacgtgcc cgcgtctcac gtccttctgc ggacaccatc atcggcgagc tttccggcat
      421 ggcgaaagat ttcctttctc atcctggtgg gattgcccat ttcgaacaat tacgtctgtt
      481 ctttgaatcc agcctggtgc gctatgcggc tgaacacgcc accgatgagc aaatcgattt
      541 gctggcaaaa gcactggaaa tcaacagtca gtcgctggat aacaacgcgg cattcattcg
      601 ctcagacgtt gatttccacc gcgtgctggc ggagatcccc ggtaacccaa tcttcatggc
      661 gatccacgtt gccctgctcg actggctgat cgccgcacgt ccaaccgtgg ctgatcaggc
      721 tctgcacgag cataacaacg ttagctatca acagcatatt gcgatcgttg acgcgatccg
      781 ccgccacgat cctgacgaag ccgatcgtgc gttgcaatcg catctcaaca gcgtctctgc
      841 tacctggcac gccttcggtc agaccaccaa caaaaagaaa taatgccact ttagtgaagc
      901 agatcgcatt ataagctttc tgtatggggt gttgcttaat tgatctggta taacaggtat
      961 aaaggtatat cgtttatcag acaagcatca cttcagaggt atttatggca acgaatttac
     1021 gtggcgtaat ggctgcactc ctgactcctt ttgatcaaca acaagcactg gataaagcga
     1081 gtctgcgccg cctggttcag ttcaatattc agcagggcat tgacggttta tacgtgggtg
     1141 gttcgaccgg cgaagccttt gtacaaagcc tttccgagcg tgaacaggta ctggaaatcg
     1201 tcgccgaaga ggcgaaaggt aagattaaac tcatcgccca cgtcggttgc gtcagcaccg
     1261 ccgaaagcca acaacttgcg gcatcggcta aacgttatgg cttcgatgcc gtctccgccg
     1321 tcacgccgtt ctactatcct ttcagctttg aagaacactg cgatcactat cgggcaatta
     1381 ttgattcggc ggatggtttg ccgatggtgg tgtacaacat tccagccctg agtggggtaa
     1441 aactgaccct ggatcagatc aacacacttg ttacattgcc tggcgtaggt gcgctgaaac
     1501 agacctctgg cgatctctat cagatggagc agatccgtcg tgaacatcct gatctggtgc
     1561 tctataacgg ttacgacgaa atcttcgcct ctggcctgct ggcgggcgct gatggtggta
     1621 tcggtagtac ctacaacatc atgggctggc gctatcaggg gatcgttaag gcgctgaaag
     1681 aaggcgatat ccagaccgcg cagaaactgc aaactgaatg caacaaagtc attgatttac
     1741 tgatcaaaac gggtgtattc cgcggcctga aaactgttct gcattatatg gatgtcgttt
     1801 ctgtgccgct gtgccgcaaa ccgtttggtc cggtagatga aaaatatctg ccagaactga
     1861 aggcgctggc ccagcagttg atgcaagagc gcgggtgagt tgtttcccct cgctcgcccc
     1921 taccgggtga ggggaaataa acgcatctgt accctacaat tttcatacca aagcgtgtgg
     1981 gcatcgccca ccgcgggaga ctcacaatga gtactacaac ccagaatatc ccgtggtatc
     2041 gccatctcaa tcgtgcacaa tggcgggcat tttccgctgc ctggttggga tatctgcttg
     2101 acggttttga tttcgtttta atcgccctgg tactcaccga agtacaaggt gaattcgggc
     2161 tgacgacggt gcaggcggca agtctgatct ccgcagcctt tatctctcgc tggttcggcg
     2221 gcctgatgct cggcgctatg ggtgaccgct acgggcgtcg tctggcaatg gtcaccagca
     2281 tcgttctctt ctcggccggg acgctggcat gcggctttgc gccaggctac atcaccatgt
     2341 ttatcgctcg tctggtcatc ggcatgggga tggcgggtga atacggttcc agcgccacct
     2401 atgtcattga aagctggcca aaacatctgc gtaacaaagc cagtggcttt ctgatttcag
     2461 gcttctctgt aggggccgtc gttgccgctc aggtctacag cctggtggtt ccggtctggg
     2521 gctggcgtgc gctgttcttt atcggcattt tgccaatcat ctttgctctc tggctgcgta
     2581 aaaacatccc ggaagcagaa gactggaaag agaaacacgc aggtaaagca ccagtacgca
     2641 caatggtgga tattctctac cgtggtgaac atcgcattgc caatatcgta atgacactgg
     2701 cggcggctac tgcgctgtgg ttctgcttcg ccggtaacct gcaaaatgcc gcgatcgtcg
     2761 ctgttcttgg gctgttatgc accgcaatct ttatcagctt tatggtgcag agtacgggca
     2821 aacgctggcc aactggcgta atgctgatgg tggtcgtgtt gtttgctttc ctctactcat
     2881 ggccgattca ggcgctgctg ccaacgtatc tgaaaaccga tctggcttat aaccctcata
     2941 ctgtcgccaa tgtgctgttc tttagtggct ttggcgcggc ggtggggtgc tgcgtcggtg
     3001 gcttcctcgg tgactggctg ggaacccgca aagcgtacgt ttgtagtctg ttggcctcgc
     3061 agctgctgat tattccggta tttgctattg gcggtgcaaa cgtctgggtg ctcggtctgt
     3121 tactgttctt ccagcaaatg cttggacaag ggatcgccgg gatcttaccg aaactgattg
     3181 gcggttattt cgataccgac cagcgtgcag cgggcctggg ctttacctat aacgttggcg
     3241 cattgggtgg cgcactggcc ccaatcatcg gcgcgttgat cgctcaacgt ctggatctgg
     3301 gtactgcact ggcatcgctc tcgttcagtc tgacatttgt ggtgatcctg ctgattggtc
     3361 tggatatgcc ttctcgtgtt cagcgttggc tgcgcccgga agcgttgcgt actcatgacg
     3421 ctatcgacgg caaaccattt agcggtgccg tgccgtttgg cagcgccaaa aacgatttag
     3481 tcaaaaccaa aagttaatcc tgttgcccgg tctctgtatc gggcctttcg ctaagggaag
     3541 atgtatgtcg ttacttgcac aactggatca aaaaatcgct gctaacggtg gcctgattgt
     3601 ctcctgccag ccggttccgg acagcccgct cgataaaccc gaaatcgtcg cagccatggc
     3661 attagcggca gagcaggcgg gcgcggttgc cattcgcatc gaaggtgtgg caaatctgca
     3721 agccacgcgt gcggtggtga gcgtgccgat tattggaatt gtgaaacgcg atctggagga
     3781 ttctccggta cgcatcacgg cctatattga agatgtcgat gcgctggcgc aggcgggcgc
     3841 ggacattatc gccattgacg gcaccgaccg cccgcgtccg gtgcctgttg aaacgctgct
     3901 ggcacgtatt catcatcacg gtttactggc gatgaccgac tgctcaacgc cggaagacgg
     3961 cctggcatgc caaaagctgg gagccgaaat tattggcact acgctttctg gctataccac
     4021 gcctgaaacg ccagaagagc cggatctgac gctggtgaaa aggttgagcg acgccggatg
     4081 tcgggtgatt gccgaagggc gttacaacac gcctgctcag gcggctgatg cgatgcgcca
     4141 cggcgcgtgg gcggtgacgg tcggttctgc cattacgcgt cttgaacaca tttgtcagtg
     4201 gtacaacaca gcgatgaaaa aggcggtgct atgaccacac tggcgattga tatcggcggt
     4261 actaaacttg ccgccgcgct gattggcgct gacgggcaga tccgcgatcg tcgtgaactt
     4321 cctacgccag ccagccagac accagaagcc ttgcgtgatg ccttatccgc attagtctct
     4381 ccgttgcaag ctcatgcgca gcgggttgcc atcgcttcaa ccggaattat tcgcgacggc
     4441 agtttgctgg cgctgaatcc gcataatctt ggtggattgc tacactttcc gttagtcaaa
     4501 acgctggggc aacttaccga tttgccgacc attgccatta acgacgccca ggccgcagca
     4561 tgggcagagt atcaggcgct ggaaggcgat ataaccgata tggtgtttat caccgtttcc
     4621 actggcgtcg gcggcggtgt agtgagcggc ggcaaactgc ttaccggccc tggcggtctg
     4681 gcggggcaca tcgggcatac gcttgccgat ccacacggcc cagtctgcgg ctgtggacgc
     4741 acaggttgcg tggaagcgat tgcctctggt cgcggtattg cagcggcagc gcagggggag
     4801 ttggctggcg cggatgcgaa aactattttc atgcgcgccg ggcagggcga cgagcaggcg
     4861 cagcagctga ttcaccgctc cgcacatgtg ctggcaaggc tgatcgctga tattaaagcc
     4921 acaactgatt gtcagtgcgt ggtggtcggt ggcagcgttg gtctggcaga agggtatctg
     4981 gcgctggtgg aaacgtatct ggcgcaggag ccagcggcat ttcatgttga tttactggcg
     5041 gcgcactacc gccatgatgc aggtttactt ggggctgcgc tgttggccca gggagaaaaa
     5101 ttatgatgat gggtgaagta cagtcattac cgtctgctgg gttacatcct gcgttacagg
     5161 acgcgttaac gctggcatta gctgccagac cgcaagaaaa agcgccgggt cgttacgaat
     5221 tacagggcga caatattttt atgaatgtca tgacgtttaa cactcaatcg cccgtcgaga
     5281 aaaaagcgga attgcacgag caatacattg atatccagct gttattaaaa ggtgaggaac
     5341 ggattctgtt tggcacggca ggcactgcgc gccagtgtga agagttccac catgaagatg
     5401 attaccagct ttgcagcgcc attgaaaacg agcaaaccat caacttaaaa ccgggaatgt
     5461 tcgccgtgtt tatgccggga gaaccgcata aaccaggatg cgttgtcggc gaacctggtg
     5521 agattaaaaa ggtggtggtg aaggttaagg ctgatttaat ggcttaaaac ttagttcgtt
     5581 ttaaagattc tgcagacagt gtctgcagaa ttcgaaaccg ctgaaattaa cggagtctat
     5641 agccttcttc tagcaccgcc tgtgttcgaa cgagttcatc cgcgagtcgt ttttcatcag
     5701 gtagttgcat cttatattca ctcgccagaa cggtgttagg caaaccagcc agtgcataat
     5761 gcgcttctcc gactcctttc tctgcacaga gaaccagacc gatgggcgga ttttcatccg
     5821 gtagcgtcca gtgctctttg gcgtagttga gatacatatt catctgtccg gcatcgctat
     5881 agctgaattt gcccactttt agatcgacga ttagcaggca gcgtaaacgg cggtggaaaa
     5941 acagcagatc gacacgaaac cagttgtcat caatgcgtaa ccttcgctgc cgaccaacaa
     6001 aggcaaaatc atccccaagt tccagcatga aatccatcag gtggttgatc agcgcctcct
     6061 caaaatcgga ttctgagtat tcatctttca attccagaaa ttcgagcaca aagggatcgc
     6121 gtatcgcctg ttgcggaaga atatgcgtct cggcaggagc gtgttgttgc agcatggctg
     6181 atttgtcatg tgacagcagt gttcgctcat aaaattgggt cgcaatttgc cgttctagct
     6241 ggcgaacaga ccagccacag cggagcgtct ctttttcata aaagctgcgg gtgtcagcgt
     6301 ttttaacgga aagtaaacgg acgtaagtgg accagggcag gtgttaattc gccagacact
     6361 gtctggcgaa tttcaacatg ttgaaaaaag aggtaaaaaa gcctcatttg gcgtaagttt
     6421 tttgcagaga acccacgctt ataccttaga cttaaatcct ttgatagtcg cttgattagc
     6481 tgcgcaccat acgcagccct ggcctcgcca ccttgttcaa attcgacaat tcgtcggcca
     6541 atttcccagt atgtcgcggt catcagcgcg ttaacgctac gtaccgtttc cgtccgggcg
     6601 ctatcgacca ggtgaataat accgtcgtgg atttgctggt agcctgttga ggttctctct
     6661 gagagcgatt ccattatctt tccctttata tggcgcagtg ttgaataatg gaatcagcta
     6721 tacgccgggc atctgccggt aacaatccag atgtgcgtat tacattggtt ttcttcagat
     6781 tatgcgaggc gctttccaga acgaaaggag aatttcagcg ccgactcttt tctgaggagg
     6841 cggcgctggg acagagctta aacttccagc cagttcataa tgccgtctgc cgccttacga
     6901 ccttcggcaa tagcggtaac gaccagatcg gaaccacgga cgatatcgcc gccagcaaag
     6961 attttcgggt tgctggtctg gaaagcgttg tcgctgcctt ccggggcgat gatgcggcct
     7021 tgcgaatcca gctcgacgct gtgttttgcc agccattcca tgctgtgtgg acggaaacca
     7081 aacgccatga tcaccgcatc tgctggaacg atatgttcgg aacctgccac gatctccgcg
     7141 cggcgacggc ctttggcatc cggttcgccc atttcagtac gcaccatttt tacgccgctg
     7201 actttgccgt taccgttcac ttcaattccc agtggctgga cattgaattt gaactctaca
     7261 ccttcttccc gcgcgttttt cacttcgcgg cgggaacccg gcatgttctc ttcatcacga
     7321 cgataggcac aggtaacgtg ctttgccccc tggcgtacag acgtgcgcac gcagtccatt
     7381 gcggtatcgc caccgccaag tactaccacg cgtttgcctt ccatgctgac gaacggttct
     7441 tcacgagttt cgccaaagcc cattaactgt ttggtgttgg cgataaggaa cggcagcgct
     7501 gcgtacacgc catcggcgtc ttcgttttcc agcccgccgc gcattgactg ataagtcccg
     7561 acgccaagga acacggcatc gtaatcactc agcagatcgt ccagctgtac gtcgcggccc
     7621 acttcggtat tgagtttgaa ttcaataccc atgccggtga agatttcacg gcggcgcgtc
     7681 attacctctt tttccagttt gaaagccgga ataccgaagg tcagcaagcc gccaatttcc
     7741 gggtgacggt cgaagacgac cgcttgtacg ccgttgcgcg tcaggacatc tgcacacgcc
     7801 agacctgccg gacctgcgcc gataatcgcc acttttttac cggtctgttt cacgccggac
     7861 atatccggac gccagcccat ttcgaacgct ttatcattga tataacgctc aatgttgccg
     7921 atggtcaccg cgccaaactc atcgttcagg gtgcaggaac cttcgcacag acggtcttgt
     7981 gggcagacac gtccgcatac ttccggcaga gtgttggtct ggtgcgacag ttctgccgct
     8041 tcaaaaatac gcccctcgtt ggcgagcttc agccagttcg ggatgtagtt gtgtaccggg
     8101 catttccact cgcagtatgg gttgccgcac gacaggcagc ggtcagcctg cgctttggcc
     8161 tggccttcgg aaaacggctc gtaaatttca acaaactcaa ttttgcggat cttcagcggt
     8221 ttctttggcg gatcaacgcg ctgcaggtcg ataaattgat aaacattctg actcattgtt
     8281 gctacccctt actgcgcctg cacgcgcaac tctgctgcgc tacgactacg atgacccagc
     8341 agtgctttga catcactgga cttcggttta accagcgcaa atttagtggc gaaggttgac
     8401 cagttcgcca gaatctcttc accacgctgg gaaccggtat gctgcacatg ctcggtgata
     8461 agaccacgca gatgctcttc atggatcgcc aaatcgtcaa cgcttaatac ttccaccagt
     8521 tccgggttaa cgcgtttgcg gaaatcgccg ctttcatcga gaacgtaagc gaagccgccg
     8581 gtcatacccg caccgaagtt aacgccggtt ttacccagaa tacagacgat accgcccgtc
     8641 atatattcac agccgttgtc gccaatgcct tctaccacgg tgattgcacc ggagttacgc
     8701 acgccgaaac gttcacccgc gcggcctgcg gcatacagac gaccacccgt cgctccatac
     8761 aggcaggtgt tgccgataat acttgcttca tggctgcgga aggcggaacc aaccggagga
     8821 cgaatggcga ttaagccgcc cgccatgcct ttaccgacat agtcgttggc atcaccggtc
     8881 aggtacagtt ccacgccgcc cgcgttccac acgccgaagc tctggcctgc ggtgccgttg
     8941 aagtacgctt taatcggatc ggctgccagc ccctgatcgc cgtgcgtctg ggcgatatag
     9001 cctgaaagcg acgcgccgac agagcggtcg gtgttgcgga tatcgaacca gaaggttttg
     9061 ctctggcgct catcgacaaa cggtttcgcc tgttgcagca actgcgcgtt cagcaggccg
     9121 ttatcaaacg gcgggttggt ttcggtgcag tagagtgcct tacctggatg cggttcggcg
     9181 gtctccagca gcttcgacag cgccagtttc tgctgtttgg cggtgaaacc gtccagctct
     9241 ttcagcaggt cggtgcgacc aatcagatcc accagacgtg ttacgccaag ctgtgccatc
     9301 agctcgcggg tttcacgggc gataaactca aagtaattcg tcaccttgaa cggcaggccg
     9361 tgatagtgat tcttacgcag tttgtcatcc tgagttgcca cacccgttgc gcagttgttc
     9421 aggtggcaaa tacgcagata tttacagccg agcgcgacca ttgggccagt gccgaagccg
     9481 aagctttctg cgccgagaat cgccgctttg atgatatcga cacccgtttt caggccgcca
     9541 tcgacctgca aacggatttt atgacgcaag ccgttggcaa ccagcgcctg ttgtgtttca
     9601 acaagtccca gctcccacgg acagcctgcg tatttcaccg atgaaagcgg acttgcgccg
     9661 gtgccgccgt catagcctgc gatggtgatt aaatcggcat aagctttcgc cacaccggtg
     9721 gcgatggtgc caacgcccgg ttcggaaacc agcttcacgg agatcatcgc tttcgggtta
     9781 acctgcttga ggtcgaaaat gagctgcgct aagtcttcga tagagtagat atcgtggtgc
     9841 ggcggcgggg agatcagcgt cacgcccggc accgaatagc gcagtttggc aatgtaagga
     9901 gtgactttgt cgcccggcaa ctgaccgcct tcgcccggct tcgcgccctg ggcgacttta
     9961 atctgaatga cgtcggcatt gaccagatac gccggagtaa ccccaaagcg accggaagcc
    10021 acctgcttga tgcgcgacac tttgttggtg ccgtagcgcg ccgggtcttc accaccttca
    10081 ccggagttcg agtttccgcc gatgctgttc atcgcttccg ccagcgcctc gtgggcttcc
    10141 gggcttaacg cgccgataga catcgccgcg gtatcgaagc gtttaaacag ttcgcttgcc
    10201 ggttcaacat cggcaatgtt gaccgcgttt tcacccggcg taattgccag cagatcgcgc
    10261 agcgtggttg ccggacgctc attaaccagc ttcgcgtatt cctgatagtc gctatactcg
    10321 ccgctctgta ccgcctgttg cagcgtgcgc accacgtccg ggttgtaggc gtggtattcg
    10381 ccgccgtgga cgtatttcag caggccgccc tggctgatgg gcttgcgcgc cagccaggct
    10441 cgtttcgaga gattcagcag atcctgctgg aagtcttcaa agctcgcccc cccaatgcgg
    10501 ctgaccgccc cctggaagca caggcccact acatcattgt gcagaccgac cgcttcaaac
    10561 agtttcgagc agcggtaaga ggcgatggtg gagatgccca ttttggacat gattttgtac
    10621 aagcctttgt tgatgccgtt acggtagttg agcatcacgg tacgataatc tttggcaatc
    10681 gcgtgggtgt ctaccaggcg gcccagcgtt tcataggcaa ggtacggata aatagccgtc
    10741 gcgccgaaac ccagcaacac ggcgaagtgg tgcggatcgc gggcgctggc ggtttcgacg
    10801 atgatgttgg catcgcaacg caggctttga tcgaccagac gggtctggat cgcgccaacc
    10861 gccattggag ccggaaccgg caggcgatct ttagcgatat tccggtcgga gagcaccagc
    10921 agcacggtgc cgctacgtac cattttttcg gctttgtcgc acagctcttt gaccgtcgct
    10981 tcgagcgtgg ttttggtgac gtcaaaggtg atatccagcg tatctgcgcg gtagtgctcc
    11041 tctttcatcg tcgtgagctg tttgaaatcg gagtagagca gaatcggcga tttaaagctt
    11101 aaacggtgcg cctggccctc tgcttcgcaa aagacgttca tttcacgacc gatactggtg
    11161 gcgagcgaca taacatgcgc ttcacgcagc gggtcgattg gcgggttagt cacctgggca
    11221 aactgctggc ggaagtagtc gtaaataatg cgcggctgac tggagagcac ggcgaatggg
    11281 gtatcatcgc ccatcgaacc gaccgcttcc tgaccgtttt cgcccagtac gcgaattacg
    11341 gagtccagct cttccgcgct gtagttaaac tgtttctggt agctggcaag cgtgtcgtcg
    11401 tccagttcgc ggctacccac ttcttcatcg ggcagatctt caaacggtac cagtcggcgg
    11461 acgtttttct ccatccactc tttataaggg tggcggcttt tcagatcgtc atcggtttct
    11521 gccgagtgca gaatacgccc actgcgagtg tcgattacca tcagttcgcc tggcccgacg
    11581 cggccttttt cgaccacttc gtcaggctgg taatcccaga taccgacttc agaggcgcag
    11641 gtgatgagct tatctttggt gatgacgtag cgcgccggac gcagaccgtt acggtcgagg
    11701 ttacaggcag caaaacgacc gtcagacatc acgatgcccg ccgggccatc ccacggctcc
    11761 atatgcatgg agttaaagtc aaagaaggca cgcagttccg ggtccatatc cgggttgttc
    11821 tgccaggcgg gtggtactaa tagacgcatg gcgcggatga tatccatccc gcctgccagc
    11881 agcagttcca gcatgttatc catcgaactg gagtcagagc cgatttcgtt gacgaacggt
    11941 gcggcgtcgt gcaggtcagg gataagcggt gtctggaatt tataagtacg cgcacgcgcc
    12001 cattggcggt taccggtgat ggtgttgatt tcaccgttat gcgccaggta gcggaacggt
    12061 tgcgccagcg gccagcgcgg tacggtgtta gtggagaagc gctggtggaa caggcaaatg
    12121 gccgattcca gacgcaggtc cgcaagatct agataaaagc gcggcagatc cgccggcata
    12181 cacagacctt tatagatgtt caccagattc gacaggctac agacgtagaa gtctttgtcg
    12241 gcttcgagac gcttttcaat gcggcggcgg gcgataaaca gacggcgctc catatcgcgc
    12301 ggacgccagc ctgccggggc gttcacaaaa atttgctcaa tgcgtggcag agaggagagg
    12361 gcgatttcac ccagcacgcc ttcgttagtg gggacatcac gccagcccac aatcgacaag
    12421 gtttcgcgtt gcagctcttc ttcaacgatg cggcgtgcgg cagcggcgag ttcaggatct
    12481 ttattcagga agagcatccc gacggcgtag tttttcgcta aacgccagcc gcgctcctgc
    12541 gcaacgatgc gaaaaaagcg atccggtttt tgtaacagca agccgcaacc gtcaccggtt
    12601 ttaccatcgg cgagaatcgc gccacggtgc tgcatgcggg ccagtgcgtg tattgcagta
    12661 cgcactacct tgtggctagg ttcgccttct atgtgggcga tcaggccgaa accacagtta
    12721 tccctctcaa gggatttatc gtacaacata tcgtgaacct cccccaggct ctgcgggaac
    12781 cccacttccg aaccgctgcg cacgggcaca gaaagagcgt ggcgacgggg tttgcgtgtc
    12841 atacgcgcgc ctcgcattcg cccccttgtt gtccttttcg catcggttaa tacggtgaag
    12901 aggggatctc aattactgca taaatatgat gagcaggctg ctcatccaga aagcttccag
    12961 cggatttcca acttatcggg aaagctgata caggtcaaat g
//
LOCUS       DAZKFZ010000123        12915 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000123 DAZKFZ010000000
VERSION     DAZKFZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..404
                     /gene="ydiV"
                     /locus_tag="ACHOQW_003136"
     CDS             <1..404
                     /gene="ydiV"
                     /locus_tag="ACHOQW_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP9574177.1"
                     /translation="LKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGN
                     STMKAVFDGLFTRVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEI
                     LAQITPFDFHALQGCLWPAVPINQITTLVQR"
     gene            467..1903
                     /gene="selO"
                     /locus_tag="ACHOQW_003137"
     CDS             467..1903
                     /gene="selO"
                     /locus_tag="ACHOQW_003137"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP9574178.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1907..2098)
                     /gene="hemP"
                     /locus_tag="ACHOQW_003138"
     CDS             complement(1907..2098)
                     /gene="hemP"
                     /locus_tag="ACHOQW_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP9574179.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2230..3276)
                     /gene="aroH"
                     /locus_tag="ACHOQW_003139"
     CDS             complement(2230..3276)
                     /gene="aroH"
                     /locus_tag="ACHOQW_003139"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP9574180.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3433..4266)
                     /gene="ppsR"
                     /locus_tag="ACHOQW_003140"
     CDS             complement(3433..4266)
                     /gene="ppsR"
                     /locus_tag="ACHOQW_003140"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP9574181.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4599..6977
                     /gene="ppsA"
                     /locus_tag="ACHOQW_003141"
     CDS             4599..6977
                     /gene="ppsA"
                     /locus_tag="ACHOQW_003141"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP9574182.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(7034..8680)
                     /gene="fadK"
                     /locus_tag="ACHOQW_003142"
     CDS             complement(7034..8680)
                     /gene="fadK"
                     /locus_tag="ACHOQW_003142"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP9574183.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(8737..9030)
                     /gene="ydiT"
                     /locus_tag="ACHOQW_003143"
     CDS             complement(8737..9030)
                     /gene="ydiT"
                     /locus_tag="ACHOQW_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9574184.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(9027..10316)
                     /gene="ydiS"
                     /locus_tag="ACHOQW_003144"
     CDS             complement(9027..10316)
                     /gene="ydiS"
                     /locus_tag="ACHOQW_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9574185.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10372..11310)
                     /gene="ydiR"
                     /locus_tag="ACHOQW_003145"
     CDS             complement(10372..11310)
                     /gene="ydiR"
                     /locus_tag="ACHOQW_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP9574186.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11330..12094)
                     /locus_tag="ACHOQW_003146"
     CDS             complement(11330..12094)
                     /locus_tag="ACHOQW_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP9574187.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            12410..>12915
                     /locus_tag="ACHOQW_003147"
     CDS             12410..>12915
                     /locus_tag="ACHOQW_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9574188.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         3107 a         3219 c         3352 g         3237 t
ORIGIN      
        1 tattaaaata tccgtttatt gaactgttga ttaatgagaa ttacccgcat ctcaatgaag
       61 gaaaagataa tcgcggtctt ttatccttat cgcaagtgta tccccttgtg ttaggtaatt
      121 tgggagcagg caacagcaca atgaaagcgg tttttgatgg tcttttcacc cgcgtaatgc
      181 tggataaaag ttttattcag caacaaatca cgcatcgctc cttcgaaccg tttattcgtg
      241 cgattcaggc gcaaatttcc ccttgttgca attgcatcat cgcggggggc attgatacgc
      301 cagaaatatt agcacagatt acccccttcg atttccatgc tctccaggga tgcctgtggc
      361 ctgccgttcc cataaatcag ataacgacgt tggttcagcg ataaccctcc tgtttgctgg
      421 tgtttaagac gagagtaacc gtctacacta tcaaacagga ggctctatga ccctgtcttt
      481 tattacccgc tggcgcgatg aattgccaga aacctataca gcactttccc ctacgccttt
      541 aaataatgcc cggctgattt ggcataatac cgaactcgct aacacgctga gtattccatc
      601 gtcgctgttt aaaaatggcg caggtgtctg gggcggcgaa accctactgc ctggtatgtc
      661 accactggcg caggtttaca gtggtcatca gttcggcgtc tgggcgggcc aactgggtga
      721 tgggcgcggc attttacttg gcgaacaact gcttgctgat ggcactacaa tggactggca
      781 tctgaaaggt gctggcctga cgccttattc gcgaatgggt gatggacggg cggtattacg
      841 ttcgacgata cgagaaagtc tcgccagtga ggcgatgcat tatctgggca ttccgacgac
      901 ccgcgcgtta agtatcgtca ccagcgattc gccagtgtat cgggaaacgg tggagccagg
      961 cgcgatgctg atgcgtgtgg caccaagtca tctgcgcttt ggtcatttcg aacattttta
     1021 ctatcgccgc gagccggata aggttcgtca gttggctgac tttgccattc gtcattactg
     1081 gtcacatctt gaagatgatg aggacaaata ccgtctctgg tttaacgatg ttgtcgcacg
     1141 taccgcatcg ttaattgccc aatggcagac ggtcggcttt gcacatgggg tgatgaatac
     1201 cgacaacatg tcgctgctgg gactgacgct tgattacggg ccgtttggtt ttcttaatga
     1261 ttacgaaccc ggttttattt gtaatcactc ggatcatcaa ggacgttaca gctttgacaa
     1321 tcaacctgct gtcgcgttgt ggattttaca gcgtctggcg cagacattgt caccatttgt
     1381 tgccgtagat gccctgaatg aggccctgga cagctatcag caggttttgt tgacgcatta
     1441 tggacaacgg atgcggcaga aactgggctt tatgacggag caaaaagagg ataacgcgct
     1501 actgaatgaa ttattcagtc tgatggcgcg agagcgcagc gattataccc gcacatttcg
     1561 catgctgagt ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgatg agtttattga
     1621 tcgtgcggca tttgatgact ggtttgcccg ttatcgaggg cgtttgcaac aagacgaggt
     1681 tagcgatagt gagcgtcagc aactgatgca aagcgttaac cccgctctgg tattgcgcaa
     1741 ctggttggcg caacgggcga ttgaagcggc agaaaagggt gatatgacgg aattgcaccg
     1801 cctgcatgag gcgttgcgaa atcctttcag cgacagagat gatgactatg tcagtcgtcc
     1861 acctgactgg ggtaaacggc tggaagtcag ctgctcgagt taaaggctac ttggtcaaca
     1921 gcagcttgcc agcctgagtt ttacggagca gatattcttg cccgtcatga tcgataatca
     1981 gtttgccatc cggacctaac agtgtctggc tggaaattcg ccgaataggc tgcggggaag
     2041 cggtcttgtg attggcatcc gtttcaggcg tgagttttct gctatccgta taacgcataa
     2101 aaattaataa taattgatat aacaatgatt cttattatca atattatggc tgaccagagc
     2161 aagcagtaat gtgttgagaa gacaacaagt tgcgcagaaa tgcccatccc atcaggaatg
     2221 ggcacgcatt cagaagcggg tatctaccgc agaggctagt ttttcgacca ggcgttcggt
     2281 atcttcccag cccagacacg ggtcggtaat ggattgaccg taagtgagcg gctgaccgcc
     2341 gacgattttt tgcgttcctt cgcgcaggaa actttccgcc ataattccag caatcgccgt
     2401 agagccattg cggatttgct gacaaatatc ctcacaaact tctaactggc gacggtgctg
     2461 cttctggcag ttaccgtggc tgaaatccac caccagatgt tcaggtaaat caaactcgtg
     2521 cagcgtatcg caggctgcgg cgatatcatc ggcatgataa ttcggttttt tgccgccacg
     2581 cataataatg tggccatacg ggttgccgct ggtctgatag atggtcatct gaccattttt
     2641 gtctggcgag aggaacatat ggctggcgcg ggctgcgcgg atagcatcca cagcaatccg
     2701 cgtattgcca tcggtaccat ttttaaaacc taccggacag gagagtgccg aagccatttc
     2761 acggtggatc tgactttcgg tagtacgtgc gccaatcgcg ccccaactga ttaaatcagc
     2821 aataaactga ccggtcacca tatcgaggaa ctcagtcgcg gttgggacgc ccagctcatt
     2881 tacctgtaaa agtaatttgc gcgccagctc cagaccgtga tttatccgat agctgccgtt
     2941 taaatctgga tcggagatta gtcctttcca gccgacaaca gttcgtggtt tttcaaaata
     3001 ggtgcgcatt acgatttcca gccgtgactg gtactggttg cgcagcgact gcagacgggt
     3061 ggcgtactcc attgcagcgg tgagatcgtg gatcgagcag gggccaataa tgaccaacag
     3121 tcgcttatct tcaccattga gtattttttc aattctgcgg cgggagtcgg tgacatgggt
     3181 ggcgacgcca ggcgttacgg gataccgtag cgcgagttcg gcgggcgtta ccaggctctc
     3241 aatacgcgca gtacggagtt cgtcagttct gttcattaca agtctcagaa atttgttgct
     3301 tctacggcag gactgccggg aagtgatgcg catcacgata aaccaatcgc tgctgatttc
     3361 aagtgcgagg tgtgtcacac ctcgccgggt ggttagcatg ataacaaaaa aataagctaa
     3421 tgcactagtt ctctagtaca ttcggcgact aaggcccatg atatcgagga tcttggtggc
     3481 aatctcttct accgaataat tggtactgtt aatccacggg atctgatttt tacggtacaa
     3541 ggcttccact tccgcgactt ccatcctgca ctgacgaagc gaggcatagc gactgttctc
     3601 ccgacgttcc tcgcgaatcg ccgccagacg ttccgggtcg atagtcaggc cgaacaattt
     3661 atgctgaagc ggtttgagcg acgcgggtag caccagatta tccatatcgt cggcaataaa
     3721 ggggtagttt gccgcgcgga taccaaattg cattgccaga tacagactgg tgggggtttt
     3781 accacagcga gaaacaccga gcaggatcac ctgagcctga tccagattgc gcaacgaaat
     3841 gccgtcatcg tgggcgaggg tgtaatcaat cgccgcaatg cgcgcatcat atttattgag
     3901 attattaggg ttaaggccat gggtacgatg agcaatcggc gttggatcca gtttcatctc
     3961 ttgttgtagc ggggcaacca gcgcctgaac gatatcctgg caaaaacctt cactttgcaa
     4021 gatgatggcg cgaatttccg gcaacacgat ggagtagaag accagcggac gcacgcctgt
     4081 ctggtgataa attgcgtcaa tctgatcctt cactgcccgt gcacggctct cattttcgac
     4141 aaacggcagc gtgatgctgc tgatagtgac gggaaattgt gacattactg cgtgtcctaa
     4201 tacctccgca gttattgccg taccatcaga aatataaaaa acgtggcgat caacagcatt
     4261 atccattttg ttcttcccgt gatgcagaca taattctatg aaagcataaa ttaaaaacgc
     4321 acagaagcgt agaacgttat gtctggttta taaaatgaac cttcaatttt attttttatg
     4381 aaaacagcat ttcattttta tggtttcgtt tataccgatg gtttatgtgg aaattgtcga
     4441 aaagagcaga tttgcgcaac gctgggatca gtcttaacaa gtaaaaaaat atatttgctt
     4501 gaacgattca ccgttttttt catccggtta aatatgcaaa gataaatgcg cagaaatgtg
     4561 tttctcaaac cgttcattta tcacaaaagg attgttcgat gtccaacaat ggctcgtcac
     4621 cgctggtgct ttggtataac caactcggca tgaaggatgt agacagggtt gggggcaaaa
     4681 atgcctccct gggtgaaatg attactaacc tttccggaat gggtgtttcc gttccgaatg
     4741 gtttcgccac aaccgccgac gcgtttaacc agtttctgga ccaaagcggc gtaaaccagc
     4801 gcatttatga actgctggat aaaacggata ttgacgatgt tactcagctt gcgaaagcgg
     4861 gcgcgcaaat ccgccagtgg attatcgaca ctcccttcca gcctgagctg gaaaacgcca
     4921 tccgcgaagc ctatgcacag ctttccgccg atgacgaaaa cgcctctttt gcggtgcgct
     4981 cctccgccac cgcagaagat atgccggacg cttcttttgc cggtcagcag gaaaccttcc
     5041 tcaacgttca gggttttgac gccgttctcg tggcagtgaa acatgtattt gcttctctgt
     5101 ttaacgatcg cgccatctct tatcgtgtgc accagggtta cgatcaccgt ggtgtggcgc
     5161 tctccgccgg tgttcaacgg atggtgcgct ctgacctcgc atcttctggc gtgatgttct
     5221 ccattgatac cgaatccggc tttgaccagg tggtgtttat cacttccgca tggggccttg
     5281 gtgagatggt cgtgcagggt gcggttaacc cggatgagtt ttacgtgcat aaaccgacac
     5341 tggcggcgaa tcgcccggct atcgtgcgcc gcaccatggg gtcgaaaaaa atccgcatgg
     5401 tttacgcgcc gacccaggag catggcaagc aggttaaaat cgaagacgta ccgcaggaac
     5461 agcgtgacat cttctcgctg accaacgaag aagtgcagga actggcaaaa caggccgtac
     5521 aaattgagaa acactacggt cgcccgatgg atattgagtg ggcgaaagat ggccacactg
     5581 gtaaactgtt cattgtgcag gcgcgtccgg aaaccgtgcg ctcacgcggt caggtcatgg
     5641 agcgttatac gctgcattca cagggtaaga ttatcgccga aggccgtgct atcggtcatc
     5701 gcatcggtgc gggtccggtg aaagtcatcc atgatatcag cgaaatgaac cgcatcgaac
     5761 ctggtgacgt gctggtcact gacatgaccg acccggactg ggaaccgatc atgaagaaag
     5821 catctgccat cgtcaccaac cgtggcggtc gtacctgtca cgcggcgatc atcgctcgtg
     5881 aactgggcat tccggcggta gtgggctgtg gtgatgcaac agaacggatg aaagacggtg
     5941 agaacgtcac tgtttcttgt gccgaaggtg ataccggtta cgtctatgcg gagttgctgg
     6001 aatttagcgt gaaaagctcc agcgtagaaa cgatgccgga tctgccgttg aaagtgatga
     6061 tgaacgtcgg taacccggac cgtgctttcg acttcgcctg cctgccgaac gaaggcgtgg
     6121 gccttgcacg tctggaattt atcatcaacc gtatgattgg cgtccaccca cgcgcactgc
     6181 ttgagtttga cgatcaggaa ccgcagttgc aaaacgaaat ccgcgagatg atgaaaggtt
     6241 ttgattctcc gcgtgaattt tacgttggtc gtctgactga agggatcgcg acgctgggtg
     6301 ccgcgtttta tccgaagcgc gtcattgtcc gtctctctga ttttaaatcg aacgaatatg
     6361 ccaacctggt cggtggtgag cgttatgagc cagatgaaga gaacccgatg ctcggcttcc
     6421 gtggcgcggg acgctatgtt tccgacagct tccgcgactg tttcgcgctg gagtgtgaag
     6481 cagtgaaacg tgtgcgcaac gacatggggc tgaccaacgt tgagatcatg atcccgttcg
     6541 tgcgtaccgt agatcaggcg aaagcggtgg ttgaggaact ggcgcgtcag gggctgaagc
     6601 gtggtgagaa cgggctgaaa atcatcatga tgtgtgaaat cccgtccaac gccttgctgg
     6661 ccgagcagtt cctcgaatat ttcgacggct tctcaattgg ctcaaacgac atgacgcagc
     6721 tggcgctcgg tctggatcgt gactccggcg tggtgtctga actgttcgat gagcgcaacg
     6781 atgcggtgaa agcactgctg tcgatggcga ttcgtgccgc gaagaaacag ggcaaatatg
     6841 tcgggatttg cggtcagggt ccgtccgacc acgaagactt tgccgcatgg ttgatggaag
     6901 aggggatcga tagcctgtct ctgaacccgg acaccgtggt gcaaacctgg ttaagcctgg
     6961 ctgaactgaa gaaataaaat aaatccccgg cggcgttttg tcgccgggtt tatgtgatcc
     7021 ccgaagatga aacttattca atctcttcac agacatcctg cgttaatcgc cgcataatat
     7081 ctttccgtaa caaaaacttt tgtattttac ctgaggcagt gcgcggtagt ttttcgatta
     7141 ccacgatatg ttcaggatat ttatattttg cgacccgttt acggctaaaa aaagccacca
     7201 cctcttccag cgataatgaa tgatgcggcg ctttcagcac gacataagcg catgatcgtt
     7261 cacctaaacg ttcatcgggc attgcaacca cacaggcatc gtgaatttta ggatgctgca
     7321 ataaaatatc ttccacttca cggctgctaa tattttcgcc gccgcggaca ataatatctt
     7381 ttttgcgtcc ggtaattttt atatagccag cctcatccat acggcagaga tcgccgctgt
     7441 aataccagcc ttcttcatcc agggcacggg cggttaattc aggttcatca aaatatccca
     7501 taaacacatt ggggccacgc gaggcttctt caccttcgca acctggcggt aaggtcttgc
     7561 gtgcgtcatc gaccacttta atctctacac ctgcggcagc gtaaccatcg gtgtgcataa
     7621 agcgcgacaa aggatcatcg agattcacca ccgcatgcgg cgaactttct gtggaaccat
     7681 aaacacttaa taatttaatg ccgcgctgct ggcattcacg cgccactttt ttggggattg
     7741 tggtaccgcc gcaaagaaag aagcgcagcg ctgaaaggtc tgcgggttgt ttctctagta
     7801 aattcaaaag atcatagaca aacggcgttg cgccgagcat acaggagcaa cgctgctgct
     7861 caagcagcgc gagacacgca tcaggagtga aaatatctaa caacacgctg cgagcgccaa
     7921 ttaagaatgg tgccgttacg ccatgcagaa agcccgttgc gtgaccaagt ggcgcaggca
     7981 tcataaagac atcctgccag gttagattca gccgcgcgca ataagcccgc tcactggcga
     8041 gaatattgtt atgcgtaagc atcacgccct ttggcagacc ctcggttccg gaggtaaaca
     8101 gcaccgcagc taattcatcg ccgtgggtcg ttatcgccgt ggtcagtggg gtattgtcgg
     8161 cgataatctg acttaatgag agggaagagg tggcgggagc cagtttgtcc acgccgacaa
     8221 tttgttgtag ttgtggaagc tgattttgca gcggcaggat taaatctacc ggacgcgttt
     8281 gtttaaacaa cgtcggtgta aagaacattt ttgcctgaca cttattaagc acccatacca
     8341 gttctgcttc ccgccaggaa ggcaacagcg gtacggaaac cgcaccgatt ttcaggcagg
     8401 caagatagat aacggtaaat tcacaccagc ctggcagttg aaatgctatg cgatcgcctg
     8461 attcaatacc cttcgctaac atccagtttg ccagacagct cgcggcgtga tcgagcgcgc
     8521 tataggtgta cgatgctcca tgattatcga ccacggcaat tttgtctggc atcgcacgag
     8581 cggtctgctg ccagtaatcg gccagcgaag catcgcccca tagcccttgc tgacgatacg
     8641 ccgcacgacg ttgttcgtta aacgttaatg tcactttcat tttggactct cgaaacagaa
     8701 catcaggccc gagatgcggg cctgtgggat gcatgattag ccgtagcgaa aatcaatacc
     8761 gaaggtgcct gcgggatatt gccactgttc gagaatagtg ttaccgcaca gcacccgaca
     8821 ggtgccgcac tccagacaac cggcggaatc aaaatgaatg tttcctgcgt catcctgctt
     8881 ataaagtccg gcagggcagg ctttcattaa tttacggaat tcattgatat cgggatttgc
     8941 cgccaaaatg atatgcgggt ggccctcatc aacatggaat ttattgacgc ctaatttgat
     9001 gtcgacgtta accgtagcgt tctggctcat agcgcggttg ctcccttaat gccatctttc
     9061 agcaagttga tcagcccaat tttcttcgcg tgtcccatga tcattttgcg taccggctgg
     9121 ttaggcttac cgtcaatggt gaacatctcg ttcatgatgt cggctaccat tcgtgggtat
     9181 tggctaaaca ggcgcgggtt ttccatcagc gccgggatct tgcgaaaatg ctgcatatca
     9241 cgcatgacgc agctttgttc cagctcacgt ttgtattgcg ccagactgct ggcggagaaa
     9301 tccgcgcgtt ctttggcggc gatcactgtt gtggcggcag cctgagccga tgcaatggct
     9361 aaatccatgc cgcggaccgt aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg
     9421 atcatcacgc catcgttaac cagttgcggt accatcgcca gaccgccttc tggcaccata
     9481 tgcgcggaat attcaagcag tttgccgccg ctaatcagcg ggcgaatggc ggggtgttgt
     9541 ttaaaatctt ccagcatttg cggcacgctt ttttgcgcat gggcgatatc agccaatcca
     9601 caaaccagcc ccaaggatat ggaatccttg ttggtataga gaaatccccc gcccatcagg
     9661 ccgtcagaag gggaaccggc aaacagccag gcggcacctt cctcgcccgt aatattaaag
     9721 cgatcgttga tctgttctgg tgtgaggcca ataacctctt taacaccaac agcgtaatga
     9781 tgcggatcgg aagcgggaac cattcccagc gagcggccaa gcatcgagtt aacgccatcg
     9841 gctaaaatca ccacattggc ttcgagaata tcatccccgg cctggactcc agtgaccttg
     9901 tttccttcac gaaccaacgc atcgacgcga actcccggga taaactgtgc gcccgcctgc
     9961 tcggcttgtt ccatcaacca cgggtccaga cgattacgca atacggtata agatgcgtgt
    10021 tgcggaacat ctggttgctc gcggtgaaaa tcgagggtaa cggcgctctc ttcggttaag
    10081 aaggagattt tctcgcgtgt gaccttgcgt tctaccggcg ctgatgctgc aaagcctgga
    10141 atgattgctt caagtgtgtg ggcataaaga cgcccgccgg tcatgttttt acatccggca
    10201 ctgtcgccgc gttctatcac caggatatcc agcccggctc gcgccatgac cagtgcggca
    10261 acgctaccag caacgcccgc accgaccaca atggcatcaa atttgtcatc cgacatgcgt
    10321 tatgctccct tcccgctgcg tttacagcgg gagatgtggt tgaaagggga attagcggct
    10381 caactggcta atcagggcag ggacgacttt gtagatatcg cccaccagac cgtagtcggc
    10441 atagttgaag attggcgcat ttttatcttt attgatggcg acaatcactt ttgcgccgtt
    10501 gccgccaacc atatgctgga tctgcccgga gatccctagc gtcaggtaga gatcggattt
    10561 cagcaacacg ccggagacac cgatataacg ttcacgctcc atccagttct cgccttcggc
    10621 aattggacgt gaacagccga cttcagcatt cagcaccgcc gccagttcgt ggaccatttt
    10681 caggtcatcc tgcgctgcca gaccacgacc gacgccaacc acacgttttg ctttgctcag
    10741 gtccacgctg cttgcggctt tcgcacggcg ttcgcgacag agaatttcat gacgcggagc
    10801 aacgtaaggt accgtttctg tcgggcactg atgagaggta tcactggtgc acggttcctg
    10861 aacaccgggt gcaagggtaa tgatcgccag cgagctgttg atcttttcct gagcgaacgc
    10921 taacccgcca tacatccggt gttcggcgca aatgtgacca tcgacaatat ccaccgccgt
    10981 ggcatcgttc accagcgccg cattcagttg cacacttaac cgtgctgcca gcgctttacc
    11041 acgtttcgtc gcggccagta gcaacatagc ggggtgttta tctttcagca gagcagcaat
    11101 gctttcggcg taattttcag tgcgttgcag cgcgtcgttt tgctcaagaa cataaataca
    11161 ttttggacca taaggcataa ctgcctgcgc ctggtcggta ttttgtacaa tggcatacac
    11221 ctgttggccc cattgctgag cgccgccaaa cagttcagca taacgttcag gattatcgct
    11281 aaagacccag acgctgttta attgactcat cgtttcttct ccgtaggctt cagttcaggg
    11341 ctttcttcag atgttccgcc agctcggcaa tggcctccgg cgaatcgtta tcgagaatga
    11401 tgtgcttacg ttctgtttgc ggcggtacgc gaatgccggt cagttcagca agtggtgcgc
    11461 tctggctcca gccaatatca cttgcctgcc actgatttac cggtttttta cccgcgccga
    11521 gaatggcttt catcgaagga atgcgtggca cgttaatatc ggaggtgacg cagagcacgg
    11581 ctggaacaga gagttcaata acttcaacgt catcttcaag cgtgcgttca atcaccagtg
    11641 tattgccctg acgctgaata gcactcactg cattaatcac cggaagttgc agaatttctc
    11701 cgaccagcaa gccaacctgc tgggcataaa ggtcgccgga acctttacca aagatcagta
    11761 aatcgaagcc gatcttttca actgctgccg ccagcgcttt tgcggtatcg agaggcagtg
    11821 catgctcaag ttgtgcatcc tgcaccatat acaggctgtg cggcccgcgg gatagcacgt
    11881 ctttgcgcac tttcgagttc tgcaacaatg agccgccaac ggtcagcgcg gctatctcat
    11941 cgtcatctgt tgcaagctgg cttgcagctt caatggcatt gagatcgaac tggctgattt
    12001 tggcgtcggc attgtcgaaa ttcagggtgt attctggagt gacaacaatg tcctgttctt
    12061 caggcaccag cttaaagcag gttattattt tcatgatttc tcctgtgagt cgcatacgga
    12121 accatctccg atggaaggta aaaatggcct gaaaatagtg tgcgatagcg gtgcggggtt
    12181 ttcgacggag caagctgttt tccttgccaa caatcttgca aaaaaaatgc gatcgctaaa
    12241 acaaaagtgc aatttgcggc aatatagtta ttccatgacc gtacaatctt gcattcagtg
    12301 taaataatct tgtttagctc tcgctaaacg cggttgtata accgcaatac acgccgtatt
    12361 ttatccggca tatattgttt ttgcttgctc atggaatagg gaggttgtca tgtatcaacg
    12421 ctgttttgat aatgccagtg aaacgctgtt tgtcgctggt aaaacgccac ggctttcacg
    12481 ttttgcattt agcgatgatc caaaatggga gtctggacat cacgttcatg acaatgaaac
    12541 cgagctgatt tacgtcaaga aaggggtggc aaggtttacc atcgattctt cgttatatgt
    12601 cgcgcatgca gatgatatag tggtgataga acgcggcagg ctgcatgcgg tggcctctga
    12661 cgttaacgat ccggcaacga cgtgtacttg tgcgctgtac ggctttcagt ttcagggggc
    12721 tgaggaaaat catctactgc aaccgcattc ttgtccggta attgccgcgg ggcagggaaa
    12781 agaagtcatt aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa gtaaaaatag
    12841 ccaaacatct tcgttatggg atgcatttgc ctatacatta gcaattcttt actacgaaaa
    12901 ctttaaaaat gctta
//
LOCUS       DAZKFZ010000124        12743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000124 DAZKFZ010000000
VERSION     DAZKFZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            64..1578
                     /gene="caiT"
                     /locus_tag="ACHOQW_003148"
     CDS             64..1578
                     /gene="caiT"
                     /locus_tag="ACHOQW_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP9574189.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1609..2751
                     /gene="caiA"
                     /locus_tag="ACHOQW_003149"
     CDS             1609..2751
                     /gene="caiA"
                     /locus_tag="ACHOQW_003149"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP9574190.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2880..4097
                     /gene="caiB"
                     /locus_tag="ACHOQW_003150"
     CDS             2880..4097
                     /gene="caiB"
                     /locus_tag="ACHOQW_003150"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP9574191.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4171..5724
                     /gene="caiC"
                     /locus_tag="ACHOQW_003151"
     CDS             4171..5724
                     /gene="caiC"
                     /locus_tag="ACHOQW_003151"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP9574192.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5833..6618
                     /gene="caiD"
                     /locus_tag="ACHOQW_003152"
     CDS             5833..6618
                     /gene="caiD"
                     /locus_tag="ACHOQW_003152"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP9574193.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6624..7214
                     /gene="caiE"
                     /locus_tag="ACHOQW_003153"
     CDS             6624..7214
                     /gene="caiE"
                     /locus_tag="ACHOQW_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP9574194.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7333..7728)
                     /gene="caiF"
                     /locus_tag="ACHOQW_003154"
     CDS             complement(7333..7728)
                     /gene="caiF"
                     /locus_tag="ACHOQW_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP9574195.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7989..11210)
                     /gene="carB"
                     /locus_tag="ACHOQW_003155"
     CDS             complement(7989..11210)
                     /gene="carB"
                     /locus_tag="ACHOQW_003155"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9574196.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11228..12376)
                     /gene="carA"
                     /locus_tag="ACHOQW_003156"
     CDS             complement(11228..12376)
                     /gene="carA"
                     /locus_tag="ACHOQW_003156"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP9574197.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3010 a         3302 c         3361 g         3070 t
ORIGIN      
        1 ttaacaaaaa taaaacaaac gggaacgcaa aataaatatt cgttttcaca gtggaattaa
       61 ctcatgaaga atgaaaagag aaaaacggga atagaaccga aggttttctt tccgccatta
      121 ataatcgtcg gcatactttg ttggcttaca gtcagagatc tggatgcggc gaatgtcgtt
      181 attaatgctg tattcagtta cgtcaccaat gtatggggat gggcatttga atggtatatg
      241 gtggtgatgc ttttcggttg gttctggctg gtgtttggcc cgtatgccaa aaagcgttta
      301 ggtaacgaac cacctgaatt tagtaccgcc agttggatct ttatgatgtt cgcctcctgt
      361 acgtctgctg ccgtactgtt ctggggatcg attgagatct actactacat ctccaccccg
      421 ccgtttggct tagaaccgaa ctcgacagga gcgaaagagt tggggctggc ttacagcttg
      481 ttcctctggg gacctctgcc gtgggccact tacagcttcc tttcagtcgc cttcgcttac
      541 ttcttctttg tccgcaaaat ggaagtgatt cgccccagct ccaccctggt gccgctggta
      601 ggtgaaaaac acgccaaagg gttgttcggc actatcgtcg acaacttcta tctcgtcgcc
      661 ttgatcttcg cgatgggtac cagtctgggc cttgccacgc cgctggtgac cgagtgtatg
      721 caatggttgt ttggcattcc gcataccctg caactggacg ctatcatcat tacctgctgg
      781 attatcctca acgccatttg cgtcgcctgc ggtctgcaaa aaggggtacg tatcgccagt
      841 gacgtgcgta gttacctgag cttcctgatg ctgggttggg tgttcatcgt cagcggtgcc
      901 agcttcatca tgaactactt caccgattcg gtggggatgt tgctgatgta tctgccgcgc
      961 atgttgttct ataccgatcc catcgctaaa ggcggcttcc cgcagggctg gaccgtgttc
     1021 tactgggcat ggtgggtgat ttacgccatc cagatgagta tcttcctcgc ccgcatctcc
     1081 cgtggtcgta ctgtgcgtga actgtgcttc ggcatggtga tgggactgac agcatccacc
     1141 tggatcctgt ggactgtact cggtagtaac actctgctgt tgatagataa aaacatcatc
     1201 aacattccaa atctgatcga acagtacggt gtggcgcgcg ccatcatcga aacctgggcc
     1261 gctctgccgc tcagcaccgc caccatgtgg ggcttcttca tcctctgctt tattgccacc
     1321 gttacgctgg ttaacgcctg ctcttatacc ctggcgatgt ccacttgccg cgaagtacgc
     1381 gatggtgaag aaccaccgct gctggtgcgt atcggttggt caattctggt tggcattatc
     1441 ggtattgttt tgctggcgct cggcggcctg aaaccgattc aaactgccat tatcgccgga
     1501 ggatgcccgc tgttcttcgt caacattatg gtgacgctct cctttattaa agacgcgaaa
     1561 cagaactgga aagattaatt aacccccaaa atatcaagag gttgaaagat ggattttaat
     1621 ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg aactgatggc cagcgaaaac
     1681 tgggaggcct attttgccga gtgcgaccgt gacagcgtct acccggaacg ttttgtcaaa
     1741 gcactggcgg atatgggtat cgacagtctg ctgatccctg aagagcacgg tggtctggac
     1801 gcggggtttg ttactctcgc cgccgtgtgg atggagctgg gacgtctggg ggcaccaacc
     1861 tatgtactgt accagttgcc gggcgggttc aataccttcc tgcgcgaagg cacacaagag
     1921 cagatcgaca agattatggc tttccgcggc accggtaagc agatgtggaa ctcagcgatt
     1981 accgaaccgg gcgcgggctc cgacgtgggt agcctgaaaa cgacttatac ccgtagaaat
     2041 ggtaagattt atcttaatgg tagtaagtgt tttattacca gtagcgccta caccccgtac
     2101 atcgtggtga tggcgcgcga cggggcttct ccggacaaac ctgtctacac cgaatggttt
     2161 gttgatatga gcaaaccggg catcaaagtg accaaacttg agaagctcgg tctgcgtatg
     2221 gatagctgct gtgaaatcac ctttgacgat gtggaactgg acgagaaaga catgttcggt
     2281 cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg accatgaacg tttcctggta
     2341 gccctgacca actacggtac ggcgatgtgc gcctttgaag atgcggcgcg ctacgccaac
     2401 cagcgcgtgc agtttggcga ggctattggt cgtttccagt tgattcagga aaaattcgcc
     2461 cacatggcga tcaaattaaa ctccatgaaa aacatgctgt atgaagcagc gtggaaagca
     2521 gacaacggca ccatcacctc tggcgatgca gcgatgtgca aatacttctg cgccaatgcg
     2581 gcatttgaag tggtggatag cgcaatgcag gtgctgggcg gtgtcgggat tgcgggcaac
     2641 caccgcatca gccgcttctg gcgtgacctg cgtgtagacc gcgtttccgg aggatctgac
     2701 gaaatgcaga tcctgacgct gggtcgtgcg gtgctgaagc aataccgcta agtccgtgcg
     2761 ctgcctgatg cgacgctgac gcgtcttatc aggcctacgg gagcacgaat gtaggccgga
     2821 taaggcgttt acgccgcatc cggcagtcat gcgctacata aatttttcag gagctaataa
     2881 tggatcatct acccatgccg aaattcgggc cgttggccgg attgcgcgtt gtcttctccg
     2941 gtatcgaaat tgccgggccg ttcgccgggc aaatgttcgc agaatggggt gcggaagtta
     3001 tctggatcga gaacgtcgcc tgggccgaca ccattcgcgt tcaaccgaac tacccgcaac
     3061 tctcccgccg caatttgcac gcgctgtcgt taaatatttt caaagatgaa ggccgcgaag
     3121 cgtttctgaa attaatggaa accaccgata tcttcatcga agccagtaaa ggtccggcct
     3181 ttgcccgtcg tggcattacc gatgaagtac tgtggcaaca caacccgaaa ctggttatcg
     3241 ctcacctgtc cggttttggt cagtacggca ccgaggagta caccaatctt ccggcctata
     3301 acaccatcgc ccaggccttc agtggttacc tgattcagaa cggtgatgtt gaccagccaa
     3361 tgcctgcctt cccgtatacc gccgattact tttctggcct gaccgccacc acggcggcgc
     3421 tggcagcact gcataaagcg cgtgaaaccg gtaaaggcga aagtatcgac atcgccatgt
     3481 atgaagtgat gctgcgtatg ggccagtact tcatgatgga ttacttcaac ggcggcgaaa
     3541 tgtgcccgcg catgagcaaa ggtaaagatc cctactatgc cggttgcggt ctgtataaat
     3601 gtgccgacgg ctacatcgtg atggaactgg tgggcattac ccaaattgaa gagtgcttta
     3661 aagatattgg cctcgcacat ctactcggta cgccggaaat cccggaaggc actcagctta
     3721 tccaccgtat cgaatgccct tacggcccac tggtagaaga aaaactcgat gcctggctgg
     3781 cggcacatac catcgcagaa gttaaagaac gctttgccga actgaatatc gcctgtgcca
     3841 aagtactgac cgtaccggaa ctggaaagca atccacagta tgttgcccgt gaatcaatca
     3901 ctcagtggca aacgatggat ggtcgcacct gcaaagggcc gaacatcatg ccgaaattca
     3961 aaaataaccc cggacaaatc tggcgcggaa tgccctcaca tggcatggac acggctgcca
     4021 ttttgaaaaa tatcggctac agcgaaaacg acattcagga gttggtcagc aaaggtctgg
     4081 ccaaagttga ggactaaacg cgtggcgtca gtcggcttgc ctgactagcg cccgcccaat
     4141 aacaaacgaa caaaaatgga tagaggtgca atggatatca ttggcggaca acatctacgt
     4201 caaatgtggg acgatctggc ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc
     4261 agcggcggag tcgttaaccg gtatagttat cttgagttaa atcaggagat taaccgcacg
     4321 gcaaacctgt tttatacgct ggggattcgc aaaggcgaca aggttgcact acatctcgac
     4381 aactgcccgg aatttatctt ttgctggttc gggctggcaa aaattggcgc gattatggtg
     4441 ccgattaacg cccgcctgtt acgcgaggaa agtgcgtgga tcctgcaaaa tagccaggcg
     4501 tgcctgctgg tgaccagtgc gcaattctat cctatgtatc aacagattca gcaagaagat
     4561 gccactcaat tgcggcacat ttgcctgaca gatgtggcac ttcccgctga tgatggcgtg
     4621 agttcgttta ctcaactgaa aaatcaacaa cctgccacct tgtgctatgc accgccgcta
     4681 tcgactgacg atacggcgga aattctcttc acctccggca ccacctcccg accgaaaggt
     4741 gtggtgatta cccattacaa cctgcgcttc gctggatatt actccgcctg gcagtgtgca
     4801 ctgcgtgacg atgacgtcta cctgacggta atgcctgcgt ttcatatcga ttgccagtgt
     4861 actgcggcga tggcggcgtt ttctgccggg gccacctttg tgctggtcga gaaatacagc
     4921 gcccgcgcct tctggggaca ggtacagaag taccgcgcca ccattaccga atgtattccg
     4981 atgatgattc gtacgttgat ggtgcagccg ccttcagcga acgatcggca acaccgcctg
     5041 cgggaagtga tgttttatct caacttgtcg gagcaggaaa aagacacatt ttgtgaacgc
     5101 ttcggtgttc gcttgctgac gtcttatggg atgacggaaa ccattgtggg cattatcggc
     5161 gatcgccctg gcgataaacg acgctggccg tcgattggtc gggcggggtt ttgctacgac
     5221 gcggagatcc gcgacgatca caatcgcccg ctcccggcag gtgagatcgg tgaaatctgt
     5281 attaaaggcg taccagggaa aaccatcttc aaagagtatt ttctcaaccc gaaagccact
     5341 gcaaaagtgc tggaagctga tggctggctg cataccggcg ataccggata ccgcgacgaa
     5401 gaaggctttt tttatttcat cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat
     5461 gtctcctgcg tggagctgga aaatatcatc gccacccatc caaaaattca ggatatcgtg
     5521 gttgtgggta ttaaagattc gattcgcgat gaagccatca aagcatttgt ggtgctgaat
     5581 gaaggtgaaa cattgagcga agaggaattt ttccgcttct gcgaacaaaa tatggcgaaa
     5641 tttaaagtgc cctcttatct ggagatcaga aaagatctgc cacgtaattg ctcggggaaa
     5701 ataattagaa agaatctgaa ataaatgaaa cggcagggaa ctaccctgcc agctaataat
     5761 cacgctctaa aacaatatgc attttttgca gggatgttgt cgtccctgaa aaagcaaaaa
     5821 tggagaaaag gaatgagtga atcattacat ctgacccgca atggatcaat tctggaaatt
     5881 actcttgatc gcccaaaagc gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa
     5941 gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg ccattattac cggtgccgga
     6001 gagaaattct tttccgcggg ctgggattta aaagcggcag cagaaggcga agcaccggat
     6061 gctgactttg gcccgggtgg ttttgcggga ttaaccgaaa ttttcaatct cgacaaaccg
     6121 gttatcgcag ctgtgaacgg ctacgccttt ggcggcggtt tcgaactggc gctggcggca
     6181 gattttattg tttgtgccga taacgccagc ttcgccctgc cggaagccaa actgggcatc
     6241 gttcctgaca gcggcggtgt gctgcgtctg ccgaagatcc tgccgcctgc catcgtcaat
     6301 gaaatggtga tgaccggcag acgaatgggc gcagaagagg cgctgcgttg ggggatagtc
     6361 aaccgcgtgg ttagccaggc ggaactgatg gataacgccc gcgaactggc tcagcagctg
     6421 gttaacagcg ccccgctggc gattgcggcg ctgaaagaga tctaccgcac caccagcgaa
     6481 atgccggtag aagaatcgta tcgctatatt cgcagcggcg tgttgaaaca ctatccatcg
     6541 gttctgcatt cggaagatgc cattgaaggg ccgctggcgt ttgccgagaa gcgcgatccg
     6601 gtgtggaaag gacgttaaca accgtgagct attacgcctt tgaggggtta attccggtgg
     6661 ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat tggcgatgtg attgtgggag
     6721 ccggtgtcta catcggccca ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc
     6781 aagcgggagc caatattcag gatggctgca ttatgcatgg ctacaccgac actgacacca
     6841 tcgttgggga aaacggccat atcgggcacg gagcgatcct gcatggttgt gtgattggtc
     6901 gcgatgcatt ggtcgggatg aacagcgtga ttatggatgg cgcggtcatt ggcgaagaga
     6961 gcattgttgc cgccatgagc tttgtcaaag caggctttca cggcgagaaa cgccagttgt
     7021 tgatgggtac gccagcccgc gctgtacgca gtgttagtga cgacgagtta cactggaaac
     7081 ggttgaatac caaagagtat caggatcttg tcgggcgctg tcatgcatcg ttacatgaaa
     7141 cacagccact gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg acggatgtga
     7201 cgcctaaacg gtgatcttgc cggatgcggc gtaaacgcct tatccaactc tgctggagag
     7261 caaccgtagg ccggataaga tgcgccagca tcgcatccgg cgaccaggtc gcacggtatc
     7321 atcgaaatga cattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
     7381 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
     7441 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
     7501 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
     7561 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
     7621 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
     7681 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
     7741 ttcacaaatt aaaagtctgg ctatgactcg ttttgaagca attaaacaag gccatattaa
     7801 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
     7861 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaacggtc
     7921 ctcatcagag aaccgtatta gttattcgat caaattagcg gatgaaaaat atccgccatg
     7981 acacgctatt atttgatctg tgcgtgcatt tcctgcaccg aaattacttt ttcagtcgca
     8041 tcggcattca gcgccatcgc ggtagcgaaa ccaccgttca gggtggtgtc ataatgcact
     8101 ttatattgca gcgcactgcg acggatcacg cgggagtctt caatcgcacg acggcctgag
     8161 gtggtgttga tgatgtaggt atattcgcca ttcttgatac ggtcctgaat gtgcggacgg
     8221 ccttcatgca ccttgtttac cagacgcgga ttgatacccg cttcgcccag cacaatcgcc
     8281 gtgccgtggg tcgcatccag ctcgaagccc tgtttcagca gttttgccgc caggtccacc
     8341 acgcgttctt tatcgccttc gcgcacggaa agcagcgcac gaccgtgttt cttcatggtg
     8401 gagttgctgc ccagctgcgc tttggcaaac gcttcagcga aggtgcggcc cacgcccatg
     8461 acttccccgg tagaacgcat ttctggccct aacagcgggt caacgcccgg gaatttattg
     8521 aacggcagca ccacttcttt caccgagtag tacggcggga taacttcttt ggttacgccc
     8581 tgctcagcca gcgatttgcc agccatcaca cgcgccgcca cttttgccag cggtacgccg
     8641 gtggctttgg agacgaacgg aacggtacgc gccgcacgcg ggttaacttc aatcaggtag
     8701 acttcgttgt ttttcaccgc aaactgcacg ttcatcaggc cgcgcacctg caattcgaag
     8761 gccagtttct gcacctgctg gcgcattaca tcctgaattt cctgacttaa ggtgtacgct
     8821 ggcagcgaac atgcggagtc accggagtgc acgcccgcct gctcgatgtg ctccatgatg
     8881 ccgccaatca gcaccatttc gccgtcgcag atggcatcca cgtcaacttc tactgcgtca
     8941 tcaaggaaat gatccagcaa cactggcgca tcgttagaca cgctaaccgc cgtctggaag
     9001 tagcgacgca ggtcagcttc gtcatagacg atttccatcg cccgaccgcc gagaacgtaa
     9061 gacggacgca ccaccagcgg gtagccaatc tcttttgcct tctcaactgc catttcaata
     9121 gcagtaacgg tggcgttcgc cggttgtttc agtttcagac ggtcaaccgc atgctggaag
     9181 cgttcacggt cttccgcacg gtcgatagca tccgggctgg tgccgataac cggtacgcca
     9241 gcggcttcca gtgcacgcgc cagtttcagc ggggtctgac cgccgtactg aacgataacg
     9301 cctttcggct tctcaatacg cacgatttcc agcacatctt ccagagttac cggctcgaag
     9361 tagaggcgat cggaagtgtc gtagtcggta gagacggttt ccgggttaca gttaaccata
     9421 atggtttcgt aaccgtcttc acgcagcgcc agcgaggcgt gtacgcagca gtagtcgaat
     9481 tcgataccct gaccgatacg gtttggaccg ccgccaagca ccatgatttt ttcacggtcg
     9541 gtagacggat tcgcttcgca ctcttcttca taagtggagt acatgtaagc ggtgtcggtg
     9601 gcaaactctg ccgcacaggt atccacgcgc ttgtagaccg ggtgcaggtc atattggtca
     9661 cgcagcttac ggatttccgc ttcgcgtacg cccgccagtt ttgccaagcg cgcatcggca
     9721 aagcctttgc gtttcagctg gcgcaggaat tcagcgttca ggccagtgat gcccacttcc
     9781 gccactttct cttccagacg caccagttct tcaatctgta ccaggaacca gcggtcaatg
     9841 ttagtcaggt tgaagacgcc gtccacagac aggcccgcgc ggaaagcatc ggcgatgtac
     9901 cagatacgct ctgcgcctgc gtctttcagt tcgcgacgga ttttggttaa cgcttccggg
     9961 tcatccaggc tcactttcgg gtcgaatcca gtcgcaccga cttccaggcc gcgcagcgct
    10021 ttttgcaggg attcctgctg cgtgcgacca atcgccatca cttcgccaac cgatttcatc
    10081 tgagtggtca gacggtcgtt agcaccggcg aatttttcga agttgaagcg aggaattttg
    10141 gtaaccacgt aatcgatgga cggctcgaag gaggccggag tacgtccgcc agtgatgtcg
    10201 ttcatcagtt catcgagggt gtaacccacc gccagtttcg ccgccacttt agcaatcggg
    10261 aaaccggtcg ctttcgacgc cagcgccgaa gaacgggaca cgcgtgggtt catttcgata
    10321 acaatcaggc gaccgttttt cgggttcacc gcaaactgga cattggaacc accggtttca
    10381 acgccgattt cacgcagcac cgccatcgag gcgttacgca tgatttgata ttctttgtcg
    10441 gtcagcgttt gggctggcgc gacagtgatg gagtcaccgg tgtggatgcc catcgcatca
    10501 aagttttcga tagagcagac gatgatgcag ttgtcgtttt tatcgcgcac cacttccatc
    10561 tcgtactctt tccagccgat cagcgactca tcaatcagca actctttggt cggagagaga
    10621 tccagaccgc gggcgcaaat ttcttcaaac tcttcgcggt tataagcgat accgccgccg
    10681 ctaccgccca tggtaaagga tgggcgaata atgcacggga agcccacgtc agcggcaacc
    10741 gccagcgctt cttccatcgt atgtgcgata ccggaacgcg cggtttccag accaattttc
    10801 ttcatcgcta cgtcgaaacg acggcggtct tctgctttat caatcgcatc ggcagtggca
    10861 ccaatcatgg tgacgccgaa ctcttccaac acgccctgac gttccagctc cagcgcgcag
    10921 ttcagcgccg tctgaccgcc catggttggc agcaccgcgt ccgggcgctc tttttcaata
    10981 atcttacgta ccacttccca gtgaatcggc tcgatgtagg tcgcatcggc catttccggg
    11041 tcggtcatga tggtcgccgg gttggagttc accagaatga cgcggtaacc ctcttcgcgc
    11101 agggctttac atgcttgcgc gccagagtag tcaaactcac acgcctgacc gataacaatc
    11161 gggcccgcac ccagaatcag gatacttttt atatctgtac gttttggcat ggctctttta
    11221 ctcctgatta cttagcggtt ttacggtact gctcaattaa ctcgataaag tggtcgaaca
    11281 acggcgcggc gtcgtgtgga ccagggctgg cttcagggtg accctggaag ctgaacgccg
    11341 gtttatcggt gcgatgaatg ccctgtaacg taccgtcgaa cagggattta tgcgtgacac
    11401 gcaggtttgc aggtaatgtt gcttcatcca ccgcaaaacc gtggttctgg gcggtgatca
    11461 tcaccacgtt tttctccaca tctttaaccg gatggttgcc gccgtggtga ccaaatttca
    11521 ttttgacagt cttcgcaccg ctcgccagcg ccagcagctg atgaccgagg cagatgccga
    11581 agaccggaat atcggtttcg aggaatttct ggatggcggt aatggcgtaa tcgcacgggg
    11641 ccgggtcgcc aggaccgttg gagaggaaga tgccgtctgg attcattttc agcacatctt
    11701 ccgcagaagt ttgcgccgga acgatggtca ggcgacagcc tctatccacc agcatccgca
    11761 ggatgttgcg tttggcacca aaatcataag ccacgacgtg gaacggcagc tcgtcttctt
    11821 ttttcgcttc tggcaggccg ccggtcaacg tccagctccc ttgtgtccag ctataggctt
    11881 ctgcggtggt cacttctttt gccagatcca tgccattcag acctgggaac gcgcgggctt
    11941 tttctaacgc cagcgccgca tccgggttat cgcctgcgat aatgcagcca ttctgtgcgc
    12001 ctttctcgcg cagtaaacgc gtcagcttac gggtatcgat atcggcaatc gccacgatgt
    12061 tatggcgctt caggtaagaa gagaggtctt cggtattacg gaagttgctg gcaatcagcg
    12121 gcaggtcgcg aatcaccaga ccttgtgcat gtacctgaga agattcttca tcggcgtcat
    12181 tagtgccgac attgccaata tggggataag taagagtaac gatttgacga gaataggaag
    12241 gatcagtgag gatttcttga taaccggtca ttgaagtatt gaaaacgact tccccaaccg
    12301 ccgaacctgt tgcccctatg gcccgaccgt gaaactgggt tccgtcttcc agaaccaata
    12361 gcgctgactt aatcaaaaca ccctccagag aatattcact cactttattt gcatattaat
    12421 tcacaatgat gacgtaaatc aatgcaaatc tgcttaccga cgaatttctg gcaaacggcg
    12481 gcattctgga gatatgggcg ctaaaagtca acttaatggc aggatttttt atattatttt
    12541 gtggctcatg catgttttac aggcattaag cgacaaaaag atcatctaac ctgttctgga
    12601 aaacgcttgc gcagcaagaa gattgcatga aataaaaacg agcaggcaaa agtggaccaa
    12661 atggtcaaaa tttacattaa gacaacaaaa atagaagaca aaacatgtaa tttttggcag
    12721 ttatagaaaa attaaaagca taa
//
LOCUS       DAZKFZ010000125        12736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000125 DAZKFZ010000000
VERSION     DAZKFZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..734)
                     /locus_tag="ACHOQW_003157"
     CDS             complement(<1..734)
                     /locus_tag="ACHOQW_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9574198.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPAQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVTNTEAITLKIDPSVVLATRKYVDDKVLELR
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPAAGNNTTRIATTEFVQTAITA
                     LINGAPDTLDTLKEIAAAINNDPKFST"
     gene            complement(731..1342)
                     /locus_tag="ACHOQW_003158"
     CDS             complement(731..1342)
                     /locus_tag="ACHOQW_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP9574199.1"
                     /translation="MSDSRLLPTGSSPLEVAAARACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWSFSVDRWDDKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYQELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGAGCYHGDALTVYPYTPEAIIVGGDYFPASAIHLIDNLRVNA"
     gene            complement(1335..2243)
                     /locus_tag="ACHOQW_003159"
     CDS             complement(1335..2243)
                     /locus_tag="ACHOQW_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFP9574200.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYSVVTGGSDE"
     gene            complement(2248..2595)
                     /locus_tag="ACHOQW_003160"
     CDS             complement(2248..2595)
                     /locus_tag="ACHOQW_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP9574201.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLALMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(2592..3227)
                     /locus_tag="ACHOQW_003161"
     CDS             complement(2592..3227)
                     /locus_tag="ACHOQW_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP9574202.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTCRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(3294..3746)
                     /locus_tag="ACHOQW_003162"
     CDS             complement(3294..3746)
                     /locus_tag="ACHOQW_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP9574203.1"
                     /translation="MNEFKRFEDRLTGLTESLSPSGRRRLSAELAKRLRQSQQRRVMT
                     QKAPDGTPYAPRQQQSVRKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(3739..4206)
                     /locus_tag="ACHOQW_003163"
     CDS             complement(3739..4206)
                     /locus_tag="ACHOQW_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9574204.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVVGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(4169..4414)
                     /gene="lysC"
                     /locus_tag="ACHOQW_003164"
     CDS             complement(4169..4414)
                     /gene="lysC"
                     /locus_tag="ACHOQW_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFP9574205.1"
                     /translation="MLCAGCTSAPPAQTPVIVANACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(4314..4739)
                     /gene="lysB"
                     /locus_tag="ACHOQW_003165"
     CDS             complement(4314..4739)
                     /gene="lysB"
                     /locus_tag="ACHOQW_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFP9574206.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRASLDRANNVASGQQ
                     TTIIMLKNQLHVALTRADKNEMAQVALRQELENAAKREAQREKAITRLLNENEDFRRW
                     YGTDLPDAVRRLHQRPACADASDCRQRLPESEPLPDAGQ"
     gene            complement(4727..5152)
                     /locus_tag="ACHOQW_003166"
     CDS             complement(4727..5152)
                     /locus_tag="ACHOQW_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFP9574207.1"
                     /translation="MKKLSLSLMLHVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRFEGREPCQS"
     gene            complement(5167..5664)
                     /locus_tag="ACHOQW_003167"
     CDS             complement(5167..5664)
                     /locus_tag="ACHOQW_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFP9574208.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(5664..5945)
                     /locus_tag="ACHOQW_003168"
     CDS             complement(5664..5945)
                     /locus_tag="ACHOQW_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9574209.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(5949..6152)
                     /locus_tag="ACHOQW_003169"
     CDS             complement(5949..6152)
                     /locus_tag="ACHOQW_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP9574210.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAMLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(6152..6661)
                     /locus_tag="ACHOQW_003170"
     CDS             complement(6152..6661)
                     /locus_tag="ACHOQW_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP9574211.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(6761..7504)
                     /locus_tag="ACHOQW_003171"
     CDS             complement(6761..7504)
                     /locus_tag="ACHOQW_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFP9574212.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLALTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(7508..8581)
                     /locus_tag="ACHOQW_003172"
     CDS             complement(7508..8581)
                     /locus_tag="ACHOQW_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9574213.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRSS
                     NQMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNREQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPTKVTAEPGA"
     gene            complement(8640..9494)
                     /locus_tag="ACHOQW_003173"
     CDS             complement(8640..9494)
                     /locus_tag="ACHOQW_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9574214.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFSRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            9668..11440
                     /locus_tag="ACHOQW_003174"
     CDS             9668..11440
                     /locus_tag="ACHOQW_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFP9574215.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNSAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFLLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            11440..12474
                     /locus_tag="ACHOQW_003175"
     CDS             11440..12474
                     /locus_tag="ACHOQW_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9574216.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
BASE COUNT         2710 a         3739 c         3477 g         2810 t
ORIGIN      
        1 gtgctgaatt tcgggtcatt gttaatggcc gcggcaattt ctttcagtgt gtccagcgtg
       61 tctggcgcgc cgttaatcag agcggtaata gcggtctgaa caaactcagt ggtcgcaatc
      121 cgcgtggtgt tatttcctgc ggcaggcgtt ggcgcttttg gttctccggt aaatgtcgga
      181 ttatgtttct gcgcatactg ggtatgagga tcctgtgcgg caatgtggtt tctcatctgg
      241 tcatccacat acagccttaa ttccaggact ttatcatcca cgtatttacg ggtcgccagt
      301 accaccgacg ggtcgatttt cagcgtgatg gcttcggtat tcgtgacaac cagaatcatg
      361 cggatagtct gggtacgacc gctgccttcc tgtaactgcg gtttgtacgt ttccgggcag
      421 ttcgccaccg caatgagtac gccttcatca tcataaagcc caatctcacg gatccagaat
      481 ccgccctcgt tctcagggat gatttgctcc gcaataatct ggctctggtt gttcgggtca
      541 acactcagaa gattcagcgg cgcgatgcgt ttctggttaa taagttttgt ctgtgccggg
      601 tcaggtgtcg gcaagacacc atttgcatca ccaacggcca tttgcgtcag attcagctta
      661 ctgccgagca tcgtcgcgtt agccagccgt gccgcgccct gattagtcag aatggcgtag
      721 tatttcactg tcatgcgttt actctcaggt tatcaattaa atgaatggcc gaggccggga
      781 aataatcccc tccgacaata atggcctccg gggtgtaggg ataaaccgtc agggcgtcgc
      841 cgtgatagca tcccgcaccg gcaaaaatgt tgccggttgt acttaaactg atagccagtc
      901 ccgtcagatg gcggcttgca ggttttgcat cagcaacgag acgctccagc tcctgataca
      961 tttcctcggt aataccctgc tcaagcacgc caacaacgat gcggaacgtc cccggctcct
     1021 cgttgagctg ccaccactcc ctcacctcaa tcagatagcc gagcggctcc accacacggc
     1081 gaatcgcacc aatagtgccc ttatggcagt gaatgaaata cgcatcgcgg ataacagcgc
     1141 gttttgtcgc ttccggccac ttatcatccc agcggtcaac cgaaaatgac cacgccagcc
     1201 acggcagcag atttgccgga caggtatccg ggttccacag ctcacgaata ctgaccggcg
     1261 ttttttcaat ttccgcacag gctcttgcgg cggcgacttc aagcggtgat gagccggtcg
     1321 gcagcaggcg cgaatcactc atccgagcct ccggtcacga cgctgtattc ggtgcagaaa
     1381 gacgcctgcg tactgttgag cacaatgtca gccagaggtg cagtcagttc gacacgttgc
     1441 acgccttcca catgcaaagc ggcataaatg gcagacagac ggatgtcgcg cccaagccgg
     1501 tgctgtgccg tgatgtacgc ttccagtttt ttcacggcag cagcgcggat gggttcgctt
     1561 tcgggacctg ggtaaaggta aagcgtggcg tttatctgat attcaacaat ggcggcagac
     1621 tgcacggtca cgcggtcggc caccggcctg acgtcctcgc cattaagggc gttacgcacc
     1681 acggccagca ggtcttcgga tgcgacgccg ttattttcac gtgacagcac ggagatagtg
     1741 acgcaggccg gagacggact ggtgacagag atatccgcga cacgcccgtc agcactgcga
     1801 ccatgatact gataggctcc caccgacccg gcgacgctta agccctcaaa cgcctgctga
     1861 atacgcagac gataatcggt gtcagactcc atcactgccg gtgtcggcgg gatagtcgaa
     1921 tcatctgccg gggtgataat caggcgtgtg gtgttgtaat tggcaccaat cacatcaagg
     1981 tcattaccgg cggcacaggc cagcatcacc gcccgtgcgg cctcattcac acgctgacgc
     2041 cagataagct cacgataagc attttcctcc agaagtttga cgagaggctc ggattccagc
     2101 gtcagggtac gggcgaccgc ctcctgctgg tcttccgggt aaagggaaat cagtgtcgcc
     2161 ttgcgttcgg caagaatggt ttcaaagtcc agctcctcga ccacatccgg tgcgggtagc
     2221 tggttcaggt cgataatcgg catggtttca actcacaggg atggttaacg aaagtggctg
     2281 gccggtgtcg ttgtgctggc cggttaacgt gaccgtcatt cgcccgtcaa aactgcgctc
     2341 agtagtgacg gatgacaggg tgacgcgggg ttcccatttc agcaccgcca tgtaacaggc
     2401 gaccttaatc tgcaactcaa gcgccggggt ctgcggctgg tcaatcatta atgccagcaa
     2461 cgagccgtaa tcacgacgca tcacccgtga gccgaccggt gtacgcagga tatcgccgat
     2521 actctggctg atatgctcaa ggtcagtgac agtcaggcca tcactgcgat tcattccgag
     2581 ataacgcgct gtcataaagg actcccggtt gtgccgccgc tgtcgccggg gtgtttatgg
     2641 gtatgcagta ccttaccgtt tgatgagagt tcaccaccgg tgtgttcaat gttgccgcgc
     2701 atcgtcccgc ccttctgtac ttccagcgtg ccggtaatca gcctgttggt gcagaccacc
     2761 tccggtgtgt ccagggtgat gcgggttgac gctttcacca tgaccaccgg caccgtggca
     2821 gtaacagaat cagaagccgt cacgctggcc gttttaattc cgcttaccgt cagtgcactg
     2881 gtttcaggtt catactcaat caccgccccg tcagggaaac ggatatgcag ggcatccgcc
     2941 gacgcagacg gcgcggggtt atcgccggaa taaatccccg gcagaacaaa cgctgtgtca
     3001 agttcaccgc ccacggccag aatcagcacc tgctccccca cggaaggtgc ccaccatgtg
     3061 cgcgaacgcc cggcacgaca agtcagccac tgaagccagt cggtgcacat gccgccggtc
     3121 tgcacacggc agcgaccggc gttaaggtcg gtttcgacga caaggccggt gcgaatcatg
     3181 ttgcgcagtg cgcgcgcgag ttcctggata tttgcgagag tgttcataac gggaaggatg
     3241 ccgccgggtc ataccggcgg caatgtgacg atgaggtgtc aggaatggca caactaacgg
     3301 tcgaggtgag ccaggataat ctcttcaatc atctgcacat cctcaccggt aaagccgagc
     3361 agaggacgcg ccggataatc aattttctta ccgtctttcc ggttttcttc cgacagaccg
     3421 aactgatgca cgctggcgat tttcggtgac ttcccgccgt aaaactccat tgatgcctgt
     3481 tccgggctgg cacggatatg caaaaaacga ctggtaataa gtttcgcaaa catttttcgc
     3541 ttaacgcgac cggtcttttt tctgacgctc tgctgctggc gtggcgcgta gggtgtgccg
     3601 tccggggctt tctgtgtcat cacccgacgc tgctgactct gccgcagacg tttcgccagt
     3661 tcggcgctca gtcgtcgacg ccctgacggt gacagcgatt cagtcagtcc ggtcagccgg
     3721 tcttcaaaac gcttaaactc attcatccca cttgctcacc agttcgccat tgatataaag
     3781 ctccatcggg cgggtgaccg gctccggcgg cggaggttcc gggatattct tcacatgcag
     3841 tgcgccgacc acctcactga ccagcgtgcg ctcggtcagc atcaggctga tgctgatatc
     3901 aaagctgctg tcattgttga tgtctgcata aaacgtgaag ccctttttct ggcctgcgtc
     3961 ggtggtcatg atgtcgggct gattttcccg cagccacgcc agcaccggca cgatgagcag
     4021 gtcaaaatca ccggtaaagt cggtcacaat gacattgagc gtgtaacgct tttcgaatga
     4081 cagcgacgcc gccagtgtgg aggcaatact cccgttatcc acgaatatcc gcagcatatc
     4141 ggggttagtt ttcagcaccg tgacggcatc agtcagcgcc ctgcgcaggc tgtcgggttt
     4201 gagcatcgtt ttcgtcctga cagtgtttaa tcatttttac ctggctggca cagcgtgcca
     4261 gcgcgttctc aagctgccgg atatcagcac ttaaatcgcc gttcgtctgc gggtcactgc
     4321 ccggcatcgg gcaaaggctc actttcgggc aggcgttggc gacaatcact ggcgtctgcg
     4381 caggcggggc gctggtgcaa ccggcgcaca gcatcaggca ggtcagtgcc gtaccagcgg
     4441 cgaaaatctt cgttttcatt gagtaacctc gtgatggctt tctcgcgctg tgcttcacgc
     4501 ttcgcggcgt tctccagctc ctgacgcagt gccacctgcg ccatctcgtt tttgtctgcc
     4561 ctggtgaggg caacatgaag ctgatttttc agcatgatga tggtcgtctg ctgcccactg
     4621 gcgacattgt ttgccctgtc cagcgaggcg cgcaggctgg cgttttcatg cttcgccaga
     4681 aacagaccgg ccaccgccag tgataacaac acaaccagca caatcatcag ctttgacatg
     4741 gttcccgccc ctcaaaacgc tgacggcagg ccgtacgtat cagccggaaa aacagcgacg
     4801 ccacaagata aatcagcgcg gtaaaaatcc acccggcggc gaccagcgag ataaacgtcg
     4861 ccaccatcac caccagagcc gccgcccgtc tgcgccacgg caccggctgc aaaaacagcg
     4921 acgtgacaat cttcacggcc agcgattccg gcggcagctc ccgcccgtag cgttccagta
     4981 catactctgt ggcatacacc ccgacaccac cggcaaccac acagataacc gtcgccagaa
     5041 tcgcccaggc agcgacaaaa ctgacggcca cgctctgcgg ataaatcagg gacagtgcca
     5101 gcatcagcgc cagcgacacg tgcagcatca gtgaaaggga taatttcttc atggtgttta
     5161 ctccgtttaa gccggtacgc cgccagcggt acgccagacg gtgaccagtt tttccagtga
     5221 atgctcacgc tgaccgtaac cggcacccgg cagggacgcc cagatattgc gacagcgtga
     5281 aatggcacgc tcaatgcgtc ccgcccggat gtcatccagt gcaccgcgtt cgcggatcaa
     5341 ctgaatggcg agcctgtcct gtgacaacgg actgaaatcc ggcagggcaa gctgtttgcg
     5401 gtaatgcggc cagaacaggt aaagctgctg atagcgaccg gaggccgtgg atttttcacc
     5461 gcgacggtta aacaccttcg ccggtcggcc atgcgcgaac gggtggtcac tgtagtcggt
     5521 gaaaatttcc ggctttccgt ccagtccggt gactatcacg tcatagcccc ggtttttcgt
     5581 cagcggatga ttcgccgtcc cttcggacac ggccagcatg tcgagaaagg cggcgatatt
     5641 ctgatgcgtg ttaattaccg gcattacggt tttcccctgc ccttaaagcg gcgctgaatg
     5701 gcaatctcaa tcacctgata accggcgata cccagcatgg agccgatacc gcacaccgca
     5761 ggcagtgaca ggtcaggaaa ctgcaccaga acaacaccgg caaccatcga gacaaaacca
     5821 ccgagcaaca tgcgcccgat aaacagacgc ggggtgatgg gttcaccacc ggcaagcacc
     5881 ttgccgacaa caatcagcac tccaatcatg aaaagcgaca ggacgctttt ttcttctgct
     5941 gtcatgcgtt actcccacag attgacagtt tcagccacgg gcgcggtctg aacgtcgggc
     6001 agttcgacgg cggtgccgtg cggcagcatc gcacccagtt cagccagtcc cggatttgcg
     6061 gcgagcacgg tctcaaccac gccctcagtg cgcccgtaat accggacaca aatggcgtcg
     6121 agcgtgtcgc cctgtagcgc aaaggtcttc atcagatttg actcacgatg cagcgcggct
     6181 tgtcctggat acgtgccacc gcccagcgca tatcccgcca cagttcatca atggtgctgt
     6241 caatgctgtc ggccttcttg tcgcccttgg cactggcatc cacgccgcga taacgctcat
     6301 aaagcgatgc ggtcgccatc gcacacacgg cgcgctcgta gtaaaaaact ttgatgcttt
     6361 caccgtcgat gtcgtccgcc gggacgtccg ccagacgcgt aaaaccggca gcaattttct
     6421 gttcgcggta ctcgtacagc tccgcattcg tttccgccat gcctgacttg atggcctcac
     6481 gcagacgggc gggggcgacg gtctgctcaa ggcgcatacg ttcccggacg cgcttcgggt
     6541 cgatatcggg aaaaaagaac gtgtttttaa tcaccggctc gtcgcctgcc ggttgcggga
     6601 tgaccaccgt accctcaccg gacacgggag cctcctttcg cggaataatc agcgtcatca
     6661 tgactacctc tgaaaagtcg ggcggtggac gccggtgcag tgtcaggtga ttcaccctca
     6721 ctgaccggcg tgccgccctg gcgcggggcg cattcggttg ttaactagct ttctttttcg
     6781 ggcgtccacg ttttgccggt gtcacgctcc gggtcttacg cggggcgcgg gtggccgctt
     6841 tgggctgcgg ctccggcttc ggtttcagct cccgctccag tcgttcaatc tcttttttga
     6901 cgcctgcctg acagtcgagc tgtgtcgcac gttgcaggtg agccagcgca ccggcggcat
     6961 caccagcgtc acgcagaaac agaccggtga ttttgtgcag ctttgcacgc acttcatcag
     7021 gcatgtctgc cgtggcggtc agtgcaaggg tctccgtcag caggcgggta tccacagact
     7081 caccggcagc gtgggcgcgc atggccgcga gcgccacctc ctcggtgaac atatacggcg
     7141 gggtgcggcg gtgtttaccc ggcatggtca gaccgtactt cagggcataa cgggcaatct
     7201 ccagcgcacc ggcaatatcg ccggtatcca gacgccacag catgaccgtc atcagaatgt
     7261 catcctgtgc acctttgccc tgctccagca cgccgttcac ccacggcaac cagaacggca
     7321 gcagttcgcg ttttttcgcg gccttcagct cttttgaata aatcgctttc agtgtgcgct
     7381 ggtctgcggc cagcttgacc agcatctgct catagacagt tgcatgtcgc agcggggcgg
     7441 cgtcccgctg cgcggtcatc gctgccgaga cccgcatcat gtggcgctgt gcgggactcg
     7501 tcatcggtta cgctcccggc tctgcggtca ctttagtcgg tgtggagaag tcaccgacct
     7561 taattttttc caccagacaa ccggcggcgt agtcttccac cacgtaatca atgttcattg
     7621 actcgtagtt ctccacgcgg tcgagtttcg ggttttccac aatcacgcgg cgatggctgt
     7681 catccatgta gtagatggac aggttttcca gcttcgtgat gagcatcgca tccgccggga
     7741 agtacgggac gcgtaccgcc ggcaggttac cgatgcgttt ctggctgatg atgacgtcag
     7801 cggccagcat ctcgctgttg tcctgctccc tgttgacgat gggaaaatac ttgtccgcca
     7861 gcagctgacg ccccacaatc accacaaggt cagggtcttc ctgataccac ggctcaatca
     7921 ggttgttggt cgcatccatc accagtgcat cgaggctggc ataatcaccg cccttaccca
     7981 cgcggatgac ctcagaggtc gtgtgacctt cctcgtcagt aaccttgctc atcacgcgcg
     8041 ccggggcttc attgcggtat ttctgcagcc agccgaccgc cacatcctgc agcatctggt
     8101 tactgctgcg gtcagaggtt tcggcacgcc tcacgccgtt aaaaccggcc atgattaaat
     8161 caagggactg gcgtttgata atggcgttac ggacacggag ctggaaatcc tgataacgcg
     8221 cccacaggtc aagcgttttg tagcggatat aaaaatcgaa gttaatctgg tcgcattcgt
     8281 acttgtttga cgccagcttc gagaagtcct tcggctgacg ctcggtgcca ccggcggtgt
     8341 ctgtggtgct ggcgatggag ccggtgacac caataccaat tttttcccct ttcatttcgc
     8401 tgaccggcac aatgttgatg cgggtcagaa agtcagagga ctcctgcatg gtgttcatca
     8461 gggtctgggt gaccgacggt tcaacggtga attttttcga cacatcaccg gcgtcgatgc
     8521 cgttcagttc ggcaacacgg gacaggtagg cattaaattt aaagcgggtt tcctggcgca
     8581 tagtttttcc tgaaattaag ggttaatcgt gaaggttttc ccggactggc tgacgccggt
     8641 cagcagttcg tcatcagggc gtcaccgcca ccgccggtgg ccttgctgcg gcgctgctgg
     8701 gtcagacttt cggtgtggtc gagactgttt ttcaggcggc tgaatgcctg gctggtttca
     8761 tccgccctgt cagtcacctc ctgcttaagt gcggaaaagg cggtttccat ctcagcgagt
     8821 cgctgctcag tggcgctcag cttttcctgc acatgttcag caacagcggt caccgcttca
     8881 tgcacgtcat tcagacgggc gtcatcgctg gcctgtttgc ggccaaaaat ggatttcacc
     8941 ttttcggtca gggcggtgaa cacggtttca ggcaggtctt caaattccag ctcaacaggc
     9001 gttgctactg aaatcaggtt ttcagggctt aatttgaagc ggttcagggg gttgtgtttt
     9061 gccgtgcggc agaattccag gtattccgtg ccgaggcttg ccgggtcatc ggtgacggcc
     9121 agacccacca gataacattt gccggtgttg gcaaagttcg gctgaatttc cattgaggtg
     9181 tagaccttct gcgcggcctt gttcatcgcg ataaggtcat cggtcggggt gattttcgca
     9241 aacagcgccc atttgccttt cagcgccgaa tcgtcatcaa tcttttcggc cttcagttcg
     9301 gccacatcgc cataacgttt aaaaataccg tcaggcagga tgccgcgcag atgttccagg
     9361 ttaatgcggc aaccatagac acgcgggtca aaggtttcgg ccatttcctg aatatcctgc
     9421 gcactgatga cacgcccgtc acaggtgtca ccctcaacgc cgatacgaaa gaattttgag
     9481 actttttttg ccattgtcag gagtcctgaa tagtgattag aagagtcaca tgtcggcatc
     9541 agtttcccga cgatgcgcat cctccgccat cagtcccgga tggcttatca ctgacacaac
     9601 agcaccttag cgaatcgcgg ggcgcgactc agtagccttg ccgtgtattc atcacggcga
     9661 ggtattcatg accatcacca cagacaccac tcttttacac gacccgcgtc gtcaggcggc
     9721 gctgctgtac tggcagggat tttccgtgcc gcagattgcc gccatgttgc agatgaaacg
     9781 cccgacggtg cagagctgga aacagcgcga cggctgggac agcgttgccc ccatcagccg
     9841 tgtcgaaatg agtctggaag cgcggctgac ccagctcatc atcaaaccgc agaaaaccgg
     9901 cggtgacttc aaggaaattg acctgctcgg acgccagatt gaacgactgg cacgggtaaa
     9961 ccgctacagc cagaccggca acgaggcaga ccttaatccg aacatcgcta accgcaacaa
    10021 aggcgggcgg cgcaaaccga aaaagaattt tttcagtgac gaggctatcg aaaagctgga
    10081 gcagattttc tttgagcagt ctttcgaata tcagttgcac tggtatcgcg ccgggcttga
    10141 gcaccgcatc cgcgatatcc tgaaatcccg ccagattggc gcgacgtttt atttttcccg
    10201 cgaggcgctg ctgcgcgccc tgaaaaccgg tcataaccag atttttctgt cggccagtaa
    10261 aacgcaggcg tatgtgttcc gcgaatacat catcgccttt gcccggctgg ttgacgttga
    10321 cctgaccggt gacccgattg tcctgggcaa taacagcgca aaactgattt ttctcggcac
    10381 caactccaac accgcgcaga gccataacgg cgacctgtac gtcgacgaga ttttctggat
    10441 accgaatttt caggtactgc gtaaggtggc atcaggtatg gcctcacaga gtcacctgcg
    10501 ctcgacctat ttctccaccc cgtccacgct ggcgcacgac gcctacccgt tctggtcggg
    10561 tgaactgttc aaccggggac gcgccagcgc cgccgaacgc gtggaaatcg acgtcagtca
    10621 taacgctctt gccggtgggc ttctctgtgc ggacggtcag tggcggcaga ttgtcaccat
    10681 tgaggacgcc ctgaaaggtg gctgcacgct gttcgacatt gagcagctta aacgcgaaaa
    10741 cagcgccgac gattttaaaa acctgttcat gtgtgaattt gttgacgaca aggcgtcggt
    10801 gttcccgttc gaggagctgc aacgctgcat ggtcgacacg ctggaagaat gggaagacta
    10861 tgcgccgttt gccgcgaatc cgttcggctc ccgcccggtc tggattggtt acgacccgtc
    10921 acaccgtggc gacagtgccg gatgcgtggt gctggcaccg ccggtggtgg ccggtggcaa
    10981 attcagaata cttgagcgtc accagtggaa aggcatggac tttgccaccc aggctgaatc
    11041 catccgcaaa ctcaccgaaa aatacaacgt cgaatacatc ggaattgatg ccaccggcct
    11101 cggtgtcggc gtgttcctgc tcgttcgctc gttctatccc gccgcacgcg atatccgcta
    11161 cacgccggaa atgaaaaccg caatggtgct caaggcaaaa gacgttattc gccgtggctg
    11221 tctggaatat gacgtcagcg ccaccgacat caccagctcg tttatggcta tccgcaagac
    11281 catgaccagc agcggacgca gcgccaccta tgaggccagc cgcagcgagg aagccagcca
    11341 cgccgacctc gcctgggcga ccatgcacgc cctgttaaat gagccactca ccgccggtat
    11401 cagcaccccg ctgacatcca ccattctgga gttttactga tgagcaagaa aaaagggaaa
    11461 acaccgcaac ctgcggcaaa aaaaatgacc gccagcgccc cgaaaatgga ggcattcacc
    11521 tttggtgaac cggtgccggt actcgaccgc cgtgacattc tggattacgt cgagtgcatc
    11581 agtaacggca gatggtatga gccaccggtc agctttaccg gtctggcaaa aagcctgcgt
    11641 gctgccgtac atcacagctc accgatttac gtcaaacgta atattctggc ttcaacgttt
    11701 attccgcacc cgtggctttc ccagcaggat ttcagccgct ttgtgctgga ttttctggtg
    11761 ttcggtaatg cgtttctgga aaagcgttac agcaccaccg gtaaggtcat cagactggaa
    11821 acctcaccgg caaaatatac ccgccgtggc gtggaggagg atgtttactg gtgggtgccg
    11881 tccttcaacg agccgacagc cttcgcgccc ggctccgtgt ttcacctgct ggagccggat
    11941 attaatcagg agctgtacgg cctgccggaa tatctcagcg cccttaattc tgcctggctg
    12001 aatgagtcgg ccacgctgtt ccgccgcaag tattacgaaa acggcgctca tgccggatat
    12061 atcatgtacg tcaccgatgc cgtgcaggat cgcaacgata tcgaaatgct tcgcgaaaac
    12121 atggtcaagt cgaaaggccg caacaacttt aaaaatctgt ttctctatgc cccgcagggg
    12181 aaagccgacg gcattaaaat tatcccgctc agtgaagtgg caacgaagga cgattttttt
    12241 aatatcaaaa aagccagcgc cgctgacctg ctggacgcgc accgcatccc ctttcagttg
    12301 atgggcggca agccggagaa cgtcgggtcg ctgggtgata ttgagaaagt ggcaaaggtc
    12361 tttgtccgca atgagcttat cccgctacag gacaggatcc gcgagataaa cggctggctc
    12421 ggtcaggagg tcatccgctt taaaaactac tcactggaca ctgacaacgg ctgaacatcg
    12481 ccgcctgcgg gcggcttttt tacaccccgt catcacgccc tcacacgctc accaccgcac
    12541 aaaacacccc gcagacacac caacgcctca acgggcagac taagcgccat cacgacgcgc
    12601 tcagacgctg aaaaaataaa atcagcacca ccgccagcgc gcagtgcttt ccccgcctcg
    12661 cccgcccgct tcatgtggcg attttaatgc ggttgcattt ttatcgaaag tctcgtctca
    12721 caaatccctc ccttca
//
LOCUS       DAZKFZ010000126        12711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000126 DAZKFZ010000000
VERSION     DAZKFZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(96..278)
                     /locus_tag="ACHOQW_003176"
     CDS             complement(96..278)
                     /locus_tag="ACHOQW_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574217.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            217..2307
                     /gene="iha"
                     /locus_tag="ACHOQW_003177"
     CDS             217..2307
                     /gene="iha"
                     /locus_tag="ACHOQW_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP9574218.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLFSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2525..2675)
                     /locus_tag="ACHOQW_003178"
                     /pseudo
     CDS             complement(2525..2675)
                     /locus_tag="ACHOQW_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2954..3850)
                     /locus_tag="ACHOQW_003179"
                     /pseudo
     CDS             complement(2954..3850)
                     /locus_tag="ACHOQW_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4152..5708
                     /locus_tag="ACHOQW_003180"
     CDS             4152..5708
                     /locus_tag="ACHOQW_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9574219.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYSVLSTLLLTLILGG
                     VFWQTPVMDMGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMDILRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVTIIGGGMKAIRGVGLITL
                     MCGISTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTEPEFLIESSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTIKSAVSQIASSLTFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            5758..6477
                     /locus_tag="ACHOQW_003181"
     CDS             5758..6477
                     /locus_tag="ACHOQW_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9574220.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGAKLNGRAVYHPSCSLSRKLGVTREPLLLLENVQGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLNREGHPVKVMHIAEVLMHR"
     gene            6488..7903
                     /locus_tag="ACHOQW_003182"
     CDS             6488..7903
                     /locus_tag="ACHOQW_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP9574221.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEILGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKDLPYACSLCTACNGVCPVKIPLATLIQKHRQVMAES
                     EITPLTERRITRAFNYINSHPALWKVGMVAGAKTARLFIRKGKTPISVRAIKEWTEAR
                     DLPSPDGESFRSWFKRHKKEG"
     gene            7907..8605
                     /locus_tag="ACHOQW_003183"
     CDS             7907..8605
                     /locus_tag="ACHOQW_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9574222.1"
                     /translation="MENREEFLVAIARKLGRPVRHIPEAMPEPVNTLATTRLTELTSD
                     QRCEAFIKFASEVMLAECVLVSPENAPSTALDICWKFGSGPVIISNDQRLVDTGITPL
                     LQKEMDAALWDPEKGDENIHLAEKAKTGVVFAEYGLTESGGIVLFSAPERGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCD"
     gene            complement(9014..12268)
                     /locus_tag="ACHOQW_003184"
     CDS             complement(9014..12268)
                     /locus_tag="ACHOQW_003184"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9574223.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVLLQQAAELPPSYINPPLNLIKIVNQLKISGKD
                     NQSRIPDGILYINGLPLVVFEFKSAVREQDASIGNAWRQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKARHEVKICCRYPQYYAARKLYYSIKQARKPFGNGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTILPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVFLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVTEVKNHLDLLGQVFYDFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLERINKIKLPATKIQ
                     LLQKLLEKAISDFRKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKKNHMS"
     gene            complement(12363..>12711)
                     /locus_tag="ACHOQW_003185"
     CDS             complement(12363..>12711)
                     /locus_tag="ACHOQW_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9574224.1"
                     /translation="FFLRVNESKSSPYYLIHYFRSEKGQHALLSNASQVGVPSIAQPS
                     THLKNISFLNPPMVLLKEFEKFSTPLFHRFSKNRKCGVSLTALRNTLLPKLISGELSL
                     EDLPDLSTDTEAA"
BASE COUNT         3288 a         2831 c         3114 g         3478 t
ORIGIN      
        1 atgtggtgga aatccgcttc tacagtaacc attttttatt cgcaaaaccg aacacgccat
       61 acggatagct gttaactggc atgccgaggc agtcgttatt tatatttggt tttgtcaata
      121 atctttattt tttgtaaaag gcaaatataa attattctca ttattgtttg tatttgtgta
      181 ttgtcttgcc ggttaacatg atcggagatt agtaatatgc gaataaccac tctggcttcc
      241 gtagtcattc cctgtctcgg attttcagcc agcagcatag ctgctgcaga ggatgtgatg
      301 attgtctcgg catccggcta tgagaaaaag ctgactaacg cagccgccag tgtttctgtg
      361 attagccagg aggaattgca gtccagccag taccacgatc tggcggaggc tctgagatca
      421 gtagagggtg tggatgttga aagtggtacg ggtaaaaccg gtgggctgga aatcagcatc
      481 cgaggaatgc cagccagtta cacgctgata ctgattgatg gtgttcgtca gggcggaagc
      541 agtgacgtga ctcccaacgg tttttctgcc atgaataccg ggttcatgcc ccctctggcc
      601 gccattgagc gtattgaggt tatcaggggg ccgatgtcca cactgtatgg ctctgatgcg
      661 atgggcggtg tggtgaatat cattaccaga aagaatgcag acaaatggct ctcttccgtc
      721 aatgcagggc tgaatctgca ggaaagcaac aaatggggta acagcagcca gtttaatttc
      781 tggagcagtg gtccccttgt ggatgattct gtcagcctgc aggtacgcgg tagcacacaa
      841 cagcgtcagg gttcatcggt cacatcactg agcgatacag cagccacgcg tattccttat
      901 cccacggagt cacagaatta taatcttggt gcacgtcttg actggaaggc gtcggagcag
      961 gatgtgctct ggtttgatat ggataccacc cggcagcgtt atgataaccg ggatgggcaa
     1021 ctggggagtc tgacgggggg atatgaccgg accctgcgct atgagcgaaa caaaatttca
     1081 gctggctatg atcatacttt caccttcgga acatggaaat cgtatctgaa ctggaacgag
     1141 acagaaaata aaggtcgtga gcttgtacgc agtgtactga agcgcgacaa atgggggctt
     1201 gccggtcagc cgcgggagct taaggaatcg aaccttatcc tgaattcatt actgcttacc
     1261 cctctgggag aatctcatct ggttacggtg gggggcgagt ttcagagctc gtccatgaaa
     1321 gacggagttg tccttgccag cacaggtgaa actttccggc agaaaagctg gtcggtattt
     1381 gctgaggatg agtggcatct cacggatgca cttgcgctga ctgcgggcag ccgctatgaa
     1441 catcatgagc aattcggggg acacttcagt ccgcgtgcat atctggtctg ggatgtggca
     1501 gatgcctgga cgctgaaagg cggtgtgacc acgggatata aggcacccag aatggggcag
     1561 ctacataaag ggattagtgg tgtgtccggg cagggaaaaa caaatctact tggtaacccc
     1621 gacctgaagc cggaagagag cgtcagttat gaggctgggg tgtattacga taaccccgcc
     1681 ggtctgaatg ccaatgtcac aggttttatg actgacttct ccaacaagat tgtctcttat
     1741 tccataaatg ataacaccaa tagctatgta aacagcggaa aggcccggtt gcacggtgtg
     1801 gaatttgccg gcacattgcc gctgtggtca gaggatgtca cgctgtcact gaattacacc
     1861 tggacccgaa gtgaacaacg tgatggtgat aacaaaggtg cgccgctgag ttatacccct
     1921 gaacacatgg tgaatgcgaa actgaactgg cagatcaccg aagaggtggc atcatggctg
     1981 ggtgcccgtt atcgcgggaa aacaccacgt ttcacccaga attattcgtc actgagcgct
     2041 gtacagaaga aagtgtatga tgagaaagga gaatacctga aagcctggac ggtggtggat
     2101 gcaggtctgt cgtggaagat gacggatgcc ctgacgctga atgctgcggt gaataacctg
     2161 ctcaacaagg attacagtga cgtgagcctg ttcagtgccg gtaagagtac gctgtatgcc
     2221 ggtgattact tccagacggg atcatcaaca acaggatatg tgatacctga gcgaaattac
     2281 tggatgtcgc tgaactatca gttctgataa taacaaaacg ctatcactga cggtagaata
     2341 cgttgccact gcaactcctg gcggaacagt ggcaacgtat taggttaagt gcatttccga
     2401 tccgctaatg agatttcgtt accaacaact aatatcgtca caggaaatgc acggattatt
     2461 tttaacttat catttacata cttgtccaga gtgtaagcgc accgtgaagg acgaggggta
     2521 aaaattagtt gacagagaga gtgacgttcc agggcaacaa ctctttcatg cggttggcag
     2581 gccaggtgtt gattacactg atcacgtggc gtagccacgt ttccggctcg attccgttaa
     2641 gttttgcagc taccgatcag gctgtacatc actgccgcac tatcgctcgt catctcaaag
     2701 tccggtctcg tcagcaggaa ggtatcattc tctcccgcca tttttccgga agcccggtca
     2761 gataagtccc tttgtctatc gctgactcct ggctcataac ccggttagca gaatgcaggg
     2821 tcaccacctc gccacgaccg aatccggata agtcaattgc accttctcaa tcgccatttt
     2881 gtcacaatta agtttgataa agcaggtcac tgaggtgtac tggcaatagc ggacactacc
     2941 atttgttctt tttttaagca gccatctgat gatatttttc cctgaaggct gccggggaga
     3001 tattccccag acgagagtga cgacgctgac gattgtagaa aatctcaatg tattcccgta
     3061 ttactgagat ggcctcatcc cggctattaa aacgatagtg gctcaggctc tcatttttca
     3121 gcgttcccca gaagctttcc atcggagcgt tgtcgtaaca gttaccttta cgcgacattg
     3181 atgttttcag accaaactgc tcctggatga cccggtaatc gtatgcgcag tactgtgaac
     3241 ctcgatcgga gtggtggatt agcccggcag gtgggcgctg gctcctgagc gccataaaca
     3301 tggctttacc tgtcagctct tttgtcatgc gctctcccat ggcgtagccg acaatttcgc
     3361 acgtataaac atctttgatg ccagcgaggt acaaccatcc ctcctgtgtg gcaacatacg
     3421 tcaggtccgc cacccagacc tgatttggta ctgtaggagc gaacgtctgg ttcagcagat
     3481 ttggcgcaac tggcagattg tggctcgagt tcgtagtcgc tctgaacttg cgtttctgct
     3541 tacagcgtag ccttagctcc ttacgaagac gtgccagtcg gtcacgacca acgatgatgc
     3601 cattctctgc cagctccgtc tggagccgcc gggttccata tgtttcgcga gtgcggatat
     3661 gtgccaccgt aatctccagt tttagccgct catcactttg ttttctgtct gagggttcat
     3721 gctgtacccg gttgtaataa ccgctcctgg atacaccaaa tacctgacac atcgcttcaa
     3781 tgggaaattg ttgtcgccat tgttcgatta acgcgtattt ttcagcgact cctgtgcaaa
     3841 atacgctgtt gtagattcaa tctgtcaatg caacaccagt cttaaggtta tatcgggata
     3901 gcacacaagt gttacacgca taaacaaaaa atacccaaaa agtattattt cataacattt
     3961 attcatggaa gataacaatg aaacgattca gctatatttt ttaaactaaa aaatacttta
     4021 taagaacatc ccaattttgg aggcgatact cattcttccc ctgataaata aacactctcg
     4081 tccgacaggg cgtggaaact atcaataacc acagtaatga tgctgacttt gttttcgttt
     4141 aaggaaatga catgcacgat tacctgcttt ttttactggg acttaccccc cttctggcga
     4201 tgatcatttt gattctgaaa gtcaaaatgc ccatacacta ttctgtgctt agcactctcc
     4261 tgctcacgct aatccttgga ggggttttct ggcagacgcc agttatggat atgggatctg
     4321 ctgtggtata cggcgcaatc aaaggtttgt ggccgattgt gattgtcatc cttgctgcca
     4381 tttacagcta taacctgatg aatgcgacaa aaagcatgga tattctgaga aatgttctgg
     4441 cgagcgttag tgacgataaa cgcatccagg ttcttcttat ctcatggtgt tttggcggat
     4501 ttcttgaggc ggccgccgga tacggtacag cggtagcaat ccccataggt atactcattg
     4561 cactgggctt taacccacta aaagcagcga ttgcatcact ggttgctaat acagtcccta
     4621 cagcgtttgg ggcagtcggt atacctgtct ccgtgcttgc tgaacaagtt aatttacctg
     4681 taaccacact cagcgggacg attattcttc agctggcact gttcaacatt ctgctcccct
     4741 tcgttatcgt cacgattatc ggtggaggta tgaaagctat acgaggcgta gggttaatta
     4801 ccctgatgtg cggtattagc acattgatac ctcagtatct ggtggctgtt aatcttggcg
     4861 cagagctccc ggctttcgca ggaagtcttg tcagtctgat cgttatagtc tttatggcaa
     4921 aaaaccgtaa agaaaaaaca gaacctgaat ttcttattga gagttcagca gatagcgcca
     4981 gcgcactttc atactctcgt ggggcgttgt tacgtgcgtg ctcaatctac attctgatct
     5041 ttctgttcat tctgctttgc tctccactgt tcccgacgat aaaaagtgca gtgtctcaga
     5101 ttgcctcgtc actcaccttc tctctgagta acggcagcaa tctgtcgatg aaaattgaat
     5161 ggatcgcaac ccccggtgtt ctgattatca ttgccagtgt tattggcggc ttgatacaga
     5221 aagcatccct gtcatgcctg ttaaacgtat ttgtcagcac ggtaaaacaa cttaaaaact
     5281 ccattattgc catttctgcg attgttgcca tggcaacggt aatggatgtc tgtgggttga
     5341 ttggcactct ggcacagagc cttgtggatc tgactggtgg ggcctacctc ttcattgctc
     5401 cggttatcgg cgctcttggt acatttgtta ctggtagtga cacaaactcc aacattctgt
     5461 ttggtaaatt gcagactact gcggctgaaa aattgcatgt ggacccgctc tggctggctg
     5521 ccgcaaatac atctggcgca acaggcggca aaatgatttc tcctcagagt attgctatcg
     5581 ctgtttcagc cacccgtatg gatggtcagg gcagcgtaat catgtcagga acactgaagt
     5641 actgctttgc ttatattgtt attcttggac tgaaagtagg ccttgtctac cacctgttca
     5701 tggcctgaga aaaaacaaca aaaggacgtg gaatattaat caacaggaga aatttctgtg
     5761 aatgttaact ttttcgtcac ctgcattggt gatgcgttta aatcccggat ggcacaaaag
     5821 agtgtgctgt tactggagca attgggttgc aatgtccatt tccccgaaaa gcagggatgt
     5881 tgtggtcagc cagcgataaa tggcggttat gttcgtgatg ccatcccggg catgaaaaat
     5941 ctcatatctt cgctggaaga gaatgactgg ccggtagtat ctcctgccgg gtcatgcact
     6001 tacacgataa aaaattacgc tcgctatctc agtgaagaac ctgagtgggc ggcacgagct
     6061 gaaaaagtgg ctgaacgcct gcatgatttg acatcattta tagtaaatca tcttggcatc
     6121 acagatgttg gggcaaaact caatggtcgc gctgtgtacc atccctcctg cagcctttcc
     6181 cgtaagctgg gtgtaaccag agagccgctt ttgctacttg agaatgtaca ggggcttgag
     6241 ctactgccgt ttcacgctca ggaaacgtgc tgcgggtttg gcggcacatt ctctgtaaaa
     6301 atggcggaga tctctggtga aatggtgaaa gagaaagtcg ggcatattat ggatgttaaa
     6361 cctgattttc ttatcggggc tgacgccagt tgcctgctaa acatcggcgg tcggcttaac
     6421 cgtgaagggc atcctgtaaa agtgatgcat attgctgaag tcctgatgca cagatgaggt
     6481 aaacagaatg tctttgaaaa caagtgatat caggtttaaa gagcgtatcc gtattcagat
     6541 tcaggacccg atcatgcgaa aagctgtggc taacgcacag gagcgcatag ggactaatcg
     6601 acagaaaatg gttgatgaac tgggacactg ggaggaatgg cgttcacgag ctgaacagat
     6661 tcgtcggcat gttctggaaa atcttgatgc ttatttgtat caattgtccg aaaaggttac
     6721 tgcgaatggc gggcatgtat attttgcccg taccagagag gatgcaacca gttacatact
     6781 gaaggtggcg aaagaaaaaa acgccaggaa ggttgttaaa tccaaatcaa tggtgactga
     6841 ggaaattggc atgaatgccg ttcttcagaa agccgggata cccgtgattg aaacggacct
     6901 tggggaatat attcttcagc tagcacatga gccaccatcg catgtggttg tgccggcaat
     6961 acataaagat cgccatcaaa tccgcagagt tttacatgaa catctgggat atgacgggcc
     7021 tgaaactcca gaagcaatga cattgttcat ccgtgagaaa atcagacaag attttctgag
     7081 tgctgaagtt ggcgtaacgg ggtgtaactt tgccgttgca gaaacagggt cagtctgcct
     7141 tgttacaaac gaaggcaacg cacggatgtg caccacatta cctaaaacac atattgctgt
     7201 tatgggtatg gagcgtattg ctccgacatt ccaggaggtg gatgttctga ttaccatgct
     7261 tgcaaggagt gctgtcgggg cgagactgac aggttataac acctggctta caggccccag
     7321 agaagaagga catgttgatg gtccggaaga gttccatctc gttattgttg acaatgggcg
     7381 ttcagagatt ctcggctcag aattcagcga tgtattgcga tgtatccgct gcggtgcctg
     7441 cattaatacc tgcccggctt atcgtcagat tggtgggcat ggttatggtt caatctaccc
     7501 ggggccaatc ggcgcggtta tttccccatt gcttggtgga tatgaagact ttaaggattt
     7561 accctatgcc tgctcattgt gcactgcctg caatggggta tgtcctgtaa aaatacctct
     7621 ggcaacgcta atacaaaaac atcgccaggt tatggcagag tctgaaatca caccattaac
     7681 ggaacgccgt ataaccagag cattcaatta cataaacagc catccggcgt tgtggaaggt
     7741 aggtatggtt gctggggcca aaacagcccg tctgttcatc aggaaaggaa aaacgccgat
     7801 tagtgttaga gcgataaaag aatggactga agccagagac ctgccatcac ctgatggaga
     7861 aagcttccgt agttggttca agcgacataa gaaggagggg taatttatgg aaaacagaga
     7921 ggaatttctc gttgcgatcg ccagaaagct tggcagacca gtaagacata ttcccgaagc
     7981 aatgccggag cctgtaaata cgctggctac gacacgttta acagagttga catcagacca
     8041 acgctgtgag gcgtttatta aatttgcctc ggaagtgatg cttgccgagt gtgttctggt
     8101 gtcgcctgag aacgcaccat caacagcctt agacatctgt tggaagttcg gttccgggcc
     8161 agtcattatc agcaacgatc aacgactggt cgatacaggg attacgccat tgctacaaaa
     8221 agaaatggac gctgctctgt gggatccgga gaagggcgat gaaaatatcc atcttgctga
     8281 aaaggcaaaa actggtgtcg ttttcgctga gtatgggctg acagaatcag gaggcattgt
     8341 cttattctcc gcaccagaac gtgggcgctc agtaagcctt ttgcctgaag catcattatt
     8401 tgttttacgt aaaagcacca ttctccccag agtggcgcag cttgcgcaaa aacttcatga
     8461 tatggctcag gaaggacaac ggatgccgtc ctgtataaat ctgattggag gcccaagttc
     8521 tactgcagat atagaactga tcaaagttgt tggcgtacat ggtcctgtac atgcaaccta
     8581 tttgatcatt gatgattgcg attaacgatt gctaataaaa aaatggcctt aaggtgatat
     8641 tgcaatcgtc ttgtgaaaag gcaacaatag actaaaggtt aggcataatt gtgtttcttt
     8701 atgcctccgc agcaatttgt ggaggctttt ttgcttactc caaacagttt ggcttcttga
     8761 aaaagtcata tcccaaacct attgacgctt ataaaaaagt aaagaatggt gagtaagcgt
     8821 aaactgacca ccgtatgtag ccatgatgcc agtattggta acgtaggtgc acccctatct
     8881 atttttgtag aaccggagga aacagaccag atagtttaaa tgagctggtt atgcgtaaca
     8941 taatttatct attttggctc ttacaaaata gacaaagaag gagtatctga tgagatactc
     9001 cattatgaca gactcaactc atgtgatttt tcttaaagtt ctctgcttgc gccagtaccc
     9061 ccatataaac atcatcattc gctactggcg ggaacttatg cttgtgtaga agcagaataa
     9121 gttcaacttt cagtttcgct ttaatatcat cgcgtttact ccagtcagga tatttcgatg
     9181 tgttgtcaac cacgcttttc atctcttttg ccagcgacag cattttttca tcgtcatagg
     9241 tgaactgata tttatcgcgc atatgagcaa gaatgtcgaa gaacgctttt tcttcaatat
     9301 caatacctaa atcggcccag gtgcccattt cggttttaat atcatagata atatcggtca
     9361 tttcctgact gaatgtatcg aattcttcac cgttgagtac atcatcttct cgacgctcat
     9421 tataacgatc tataatagcc tggaagcggc gggtgaagtt aatcccttgc aactggttca
     9481 ctttcctgaa gtcgctgatc gctttttcca gtaatttttg taatagctgg atcttcgttg
     9541 ccggaagttt gatcttgtta attcgctcca gataatcttc gtcaaaaata tcgatggatt
     9601 ccgctttttt atcgccaaga aaataaattt cttctacgcc atcagctttt agcgcttctg
     9661 caatcatttc acgaacgcgt gcattcatct gggtaacatc cggtgcgtca cctttcgtca
     9721 gtttgaaaac aattgaacga acagcaagat aatagtgaat atgatcacgt tctgtctgtg
     9781 atagtgcttc actgccgcag cagacgtcgt aggcggcttt catgcgttta accagtccca
     9841 taaaacgacg ttcaactttc tgggtacgca gaacgaattc cgccgcgcgg ttgaggcagg
     9901 ataattgcgc ttgtggctca ccactaaaat aatcccgact gtcaaagtcg taaaagactt
     9961 gccccaacaa atcgagatgg tttttaactt cagtcaccga ttgctgaata tcttcaaagt
    10021 tggtggcatc aatgcgggaa tacattgcca gtgcctggtt catctgactt ttaatgccga
    10081 tgtagtccac cactaaccct ttgcttttgc cttccagttt acggttaacg cgagaaatag
    10141 tctggataag gttatgtttt tgtaagggct tatcaatata gatggtatcc agttcaggaa
    10201 catcaaaacc ggtcagccac atatcaacaa caatggcaat tttgaaattc gatttagcgt
    10261 ttttgaactg cttatccagc tctttgcgat attcttttgt gcccagtaaa tcataaagcg
    10321 cctcgtcgtc atctttaccg cgcgtcatga ccatcttcac catttcagaa ggcggtaact
    10381 ctttttgctc ctgttctgtc aggaagacgc catcgggggc ttgcttcact tcaaaccagg
    10441 cagggcgaat agctttaagc tggcggtaga aatcccaggc aatttcacgg ctggcacaaa
    10501 caaacatggc tttgcctttt acggtggaac cttcggctac gcgtttttca taatgtttgg
    10561 caaaatcttc cgcgagggct tttaatcgat cttcatcacc cagaaccgca ttcatggttg
    10621 cggtggcttt tttgctttcg tcgatttgcc actcattggt gcctgcgttt gcgcactctt
    10681 cgtaatactt ttcgacttct tccagtttgc tggagtccag gatcacttta gccgcacggc
    10741 cttcgtacac gatgcgtaca gtgatttcat cctgaacgga ttcggtcatg gtgtaactgt
    10801 cgatcacctc accgaagaca tcgagcgtcg cgtcaatcgg tgtgccggta aagccaacat
    10861 aggtggcgtt tggcagtgaa tcgtgcaggt atttcgcaaa gccatacgtt ttgcgcactt
    10921 ttccgctttc tttatcgacg atgactttct ggtcgaggtt aacctggctg cgatgtgctt
    10981 cgtccgagat gcaaatgata ttgctgcgtt ccgaaaggag ttcggtgtct tcggtgaatt
    11041 tatggatcgt tgtcaggaat acaccgccac tattgcgtcc cgccagttga ttacgcaaat
    11101 cttcacggct ggtaacggga aggatggtgt cgtcaccaat gtaatttttg gcgttgcaca
    11161 tttgcgcaga aagctgatcg tccagatcgg tgcggtcggt gatcaaaaca atggtcgggc
    11221 tggcaaactc tacgctcttc atcaaaagac gcgttaaaaa ttgcatggtg taacttttgc
    11281 cacagcccgt tgcgccaaag taagtgccgc ctttaccgtt accgaaaggt ttacgcgctt
    11341 gcttgatgct gtaatagagt ttgcgggcgg catagtactg cggatatcgg cagcaaattt
    11401 ttacttcgtg cctggcttta tccgggaagc agataaagtt tttaattaca tccagcagac
    11461 gtaccggatg aaacagcccc tgaatcattg agtgcaatga tggaattcca tcctgttcac
    11521 ggttttcatt accggtgact tttcgccatg agtaaaaata ttcatagggc gcaaacaggt
    11581 tgcccatccg gttattaact ccatcgctaa taatgcagag cgcgttgtag ataaacagtt
    11641 gcggaatatc ccggcgatag cgtttgcaga gttgtctcca ggcattgcca atactagcat
    11701 cctgctcgcg caccgcactt ttaaattcaa agacgaccag tggcagaccg ttgatataga
    11761 gaatgccgtc aggaatacga ctctgattat ctttgccgga gattttaagc tgattaacaa
    11821 tcttaatcag gttaagcggc ggattaatat aggagggcgg gagttccgca gcctgttgca
    11881 acaggacatc ttcggcgtca aatatctggc gcagtgcggc aggtagatgc cgggtgtcga
    11941 gcaattcaat gtagagatct ttttgttgcc gatcgtcgcg tttgaacaga aaaccatttg
    12001 ccagccagcg acaaaatgtt ttgttgcttt cataaagatc ggaagccgga agcgtggtga
    12061 actgtttgat cagtcgctga atctcttctt cagtaatgcc atcaggctgg tagcgtgccg
    12121 ctaaataatg acggagatcg tcttcgataa tgacctgatc gagactcgaa cgtgggacat
    12181 tatcgccgat cagatgttga tacccctgat cctgtaacag ttcaatgatc gcttgttcta
    12241 attttgcttc actgaaagag agactcatat cgcgctccaa ccctggctaa tttctggtaa
    12301 gcattcctgt atcagtagaa aaccttacca gaaagcccct gattttacag gggcaaaatg
    12361 cgttatgcgg cttctgtatc ggtgctgaga tccggaagat cttccaggga tagctcaccg
    12421 gagataagtt tcgggagcag ggtgttgcgg agggctgtta gtgagactcc acattttcta
    12481 tttttactaa agcgatggaa taaaggggtg ctaaattttt caaactcttt aagcaaaacc
    12541 attgggggat ttaggaatga tatatttttc aaatgtgttg aaggctgagc aattgaagga
    12601 acaccaacct gagaggcgtt agaaagcaga gcatgttgtc ctttttctga cctaaaataa
    12661 tgaatcaaat agtacggcga tgattttgat tcatttacgc gtaagaaaaa t
//
LOCUS       DAZKFZ010000127        12582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000127 DAZKFZ010000000
VERSION     DAZKFZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            185..1843
                     /gene="ybjD"
                     /locus_tag="ACHOQW_003186"
     CDS             185..1843
                     /gene="ybjD"
                     /locus_tag="ACHOQW_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP9574225.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1840..2796)
                     /gene="ybjX"
                     /locus_tag="ACHOQW_003187"
     CDS             complement(1840..2796)
                     /gene="ybjX"
                     /locus_tag="ACHOQW_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP9574226.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2947..4062
                     /gene="macA"
                     /locus_tag="ACHOQW_003188"
     CDS             2947..4062
                     /gene="macA"
                     /locus_tag="ACHOQW_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP9574227.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4059..6005
                     /gene="macB"
                     /locus_tag="ACHOQW_003189"
     CDS             4059..6005
                     /gene="macB"
                     /locus_tag="ACHOQW_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP9574228.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6078..6302)
                     /gene="cspD"
                     /locus_tag="ACHOQW_003190"
     CDS             complement(6078..6302)
                     /gene="cspD"
                     /locus_tag="ACHOQW_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP9574229.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6452..6526
                     /gene="yljB"
                     /locus_tag="ACHOQW_003191"
     CDS             6452..6526
                     /gene="yljB"
                     /locus_tag="ACHOQW_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP9574230.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7945
                     /locus_tag="ACHOQW_003192"
     CDS             6707..7945
                     /locus_tag="ACHOQW_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9574231.1"
                     /translation="MSRAIHRLSDTLLRKLSGSPTTKSTFFNDGGNLSVRHSTNGLLT
                     WYFTYRSGTGRQISPERLRLGNYPDLSLKAAREKASQCRAWLAEGKNPRYELNHAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGAMPLEKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVEPHVVEQLTAHQLPGMQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDKKIVVMTPRIYSQNS"
     gene            8356..8565
                     /locus_tag="ACHOQW_003193"
     CDS             8356..8565
                     /locus_tag="ACHOQW_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9574232.1"
                     /translation="MREINEDRVIREKECRELTGVCRTTRYEMEKQGRFPSRISLGGR
                     SVGWVKSEVMEWVKSRNRINSGKAA"
     gene            complement(8700..8879)
                     /locus_tag="ACHOQW_003194"
     CDS             complement(8700..8879)
                     /locus_tag="ACHOQW_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9574233.1"
                     /translation="MATGPKNAKSQSVTARIAHEIIEGMENVKEEGESTGQFINAAMR
                     GEIKRRQRRKAKEQE"
     gene            9079..9279
                     /locus_tag="ACHOQW_003195"
     CDS             9079..9279
                     /locus_tag="ACHOQW_003195"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9574234.1"
                     /translation="MRNHITHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPDGCVMVFAARIRQGEIL"
     gene            9279..9506
                     /locus_tag="ACHOQW_003196"
     CDS             9279..9506
                     /locus_tag="ACHOQW_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574235.1"
                     /translation="MHKIPFEVLIHSENALIKAREMNALLIKLIEVPEGGEESGVLMF
                     AAIQTLLSPVIDELDKAMAIHENNNVPHTGE"
     gene            9512..9811
                     /locus_tag="ACHOQW_003197"
     CDS             9512..9811
                     /locus_tag="ACHOQW_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9574236.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSREKARANIQEIKKMLNVFRGKK"
     gene            9808..11556
                     /locus_tag="ACHOQW_003198"
     CDS             9808..11556
                     /locus_tag="ACHOQW_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFP9574237.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAEQWQEQVGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQMLAGRFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPTGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTQPAPGENIRDNAPNFH
                     KWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            11845..12123
                     /locus_tag="ACHOQW_003199"
     CDS             11845..12123
                     /locus_tag="ACHOQW_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574238.1"
                     /translation="MYTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKNVEVES"
     gene            12120..12542
                     /locus_tag="ACHOQW_003200"
     CDS             12120..12542
                     /locus_tag="ACHOQW_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9574239.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLTCSQADDGT
                     ATMWLSVLAFGRQADALAKHRKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT         3291 a         2905 c         3378 g         3008 t
ORIGIN      
        1 ttatcatgct aattatttga ttttgttgtt tttgcagact tatcagcaag agggagtata
       61 acgcgattat tcgctcattt ttcagacatt tgccatgctt aaatgtgatg tcatcacgta
      121 ttagcaaggc ctttcccgtt atactgccag cgtaaaggat aagtcacata tttctggagg
      181 ggatatgatt cttgagcgcg ttgaaattgt gggttttcgc ggtatcaacc gtttgtcgtt
      241 gatgctggaa caaaacaacg tcctgattgg ggagaacgcg tggggtaaat ccagcttgct
      301 ggacgcctta accctgctgc tatcgccaga atcagatctc taccattttg agcgcgacga
      361 tttctggttc ccgccgggag atatcaacgg gcgagaacat catctgcata ttattttgac
      421 cttccgcgaa tcgctgccag gtcgacatcg ggttcgccgt tatcggccgc tggaagcgtg
      481 ctggacgcca tgcaccgatg gctatcaccg tattttttat cgtctggaag gggagagtgc
      541 ggaagacggc agcgtgatga cactgcgcag ttttctcgat aaagacggac atccgattga
      601 tgttgaggat attaacgatc aggcacacca tctggtgcgt ttaatgccgg tgctgcgctt
      661 gcgtgatgcc cgttttatgc gccgtattcg taacggcacg gtgccaaatg tccccaatgt
      721 ggaagtcacc gcgcgccagc tcgatttcct cgcccgtgag ttgtcctcac atccgcaaaa
      781 tctctctgat ggacagattc gtcagggact ttccgcaatg gtgcagttgc ttgagcatta
      841 tttctctgag cagggggctg gacaggcgcg atatcgttta atgcggcggc gggccagcaa
      901 tgagcaacga agctggcgct atctggacat catcaaccgg atgattgacc gacctggagg
      961 gcgctcgtat cgggttattt tgctcggcct gtttgctact ttattgcagg caaaaggcac
     1021 attgcgactg gataaagacg cccgtccatt gttgctgatc gaagatccag aaacgcgttt
     1081 acaccccatt atgctttcag ttgcctggca tctgttgaat cttttgcctt tacaacgtat
     1141 tgctaccacc aactctggcg agctgctttc gttaactcca gtggaacatg tttgccgcct
     1201 ggtgcgtgaa tcttctcgtg ttgccgcctg gcgtctgggg ccaagtggct tgagcaccga
     1261 agacagccgc cgcatctctt ttcacattcg ttttaatcgt ccgtcatcgc tgtttgctcg
     1321 ctgctggttg ctggtggaag gggaaacgga aacctgggtt atcaatgaac tggcgcgtca
     1381 gtgcggacat cattttgatg ccgaagggat caaggtcatt gagtttgccc agtccgggct
     1441 aaagccactg gttaaatttg cccgccgaat ggggattgaa tggcatgtac tggtcgatgg
     1501 cgatgaagca gggaagaaat atgccgctac ggtacgcagc ctgttgaata atgatcggga
     1561 agccgaacga gaacatttaa cggcgttacc ggcgctggat atggaacatt ttatgtatcg
     1621 ccagggattt tccgatgtgt tccaccgcgt ggcgcaaatc ccggaaaatg tcccgatgaa
     1681 tctgcgcaaa attatctcga aagcgatcca tcgctcttcc aaacccgatc ttgccattga
     1741 agtggcaatg gaggccggac gtcgtggtgt ggactccgta ccgacgctgc tgaaaaaaat
     1801 gttctcacgc gtgctgtggc tggcgcgcgg tcgcgcggat taaccgcgaa acatcgtggc
     1861 catttgtggc tgaatagcgt cgagcatctc atagcgccga cggtattcag cccgtttttt
     1921 actggcgatt tcggcaatct cttttcgtgc tatctgtgct ggaaggcggt aatggcgttc
     1981 agcatcacat acgccgccaa ccgattccca gaaagcgttg taatcggcat ggatcttgcc
     2041 ttctttatcg cgataacgca ggctgcggta aatatgcgtt tcattgctga cggcaataat
     2101 ctgctctacc tgcaaacgtt gggcaaacag acaggccgct tccatcacga ggcgtttggg
     2161 aaatagcccg tggcaggctt tcgtcgcatt ctggatttcc tgatgtggaa tttcccattt
     2221 tgcgccttgc agtccaccaa taaacatcgt tcttttcccc tgatattcac acagggtaaa
     2281 cgtgatttct gccagaggaa taccttcgct gttgcggaac aggattgtgc tgtcaccttc
     2341 tttatccatt gagatcatca tggtcagctc aagcgtgaac tgctcgccgt ttttgccttc
     2401 cagcttcgcc agttgcagcc cgggggtatt caaatataag ctgaattctt ccgccgacat
     2461 acatccacgg agtaacgcat aatggtaacg taacgcctcc agcaattgct tacggctaag
     2521 attcgccgca aggtaagggc gatgcagacg cacaggcagt cgcggctggc gcgttaacaa
     2581 tacattgaga ttaggccagt gggaaagttc gttcatccac tcaacgctta aacgcggcat
     2641 aatcaacgag cgcagcaaaa atttctggcg aaaactacgg cgatgccaga atttacccgg
     2701 ccgacactgt ccacgtgcca gactaagaaa aagtgacagg ctgctgagag attcagatga
     2761 cgtaaaggtc cgttcggtta gctgcgacat attcatgaaa tcaatggttg tacatgacgt
     2821 cgatttcacc attgcgtatc ttaaccaaac atcaatagtg tgattactaa cgtaaatttt
     2881 agggttttgt tgatatttcg ttgaagttaa tgacccggat tggcatatgg agtattcaga
     2941 atatttatga aaaagcggaa aaccgtgaag aagcgttacg ttattgcgct ggtgatagtc
     3001 atcgccggac tgattacgtt atggagaatt cttaacgcac ccgtgccgac ttatcagaca
     3061 ctgattgtgc gccccggtga tttacagcaa agcgtgctgg cgaccggaaa gctggacgcg
     3121 ctgcgtaagg ttgacgtggg cgcgcaggtc agcggtcagt tgaaaacgct gtcggtggcg
     3181 attggcgata aagtaaaaaa agaccagctt ttaggggtta ttgatcctga acaggctgaa
     3241 aaccagatca aggaggtcga agcaacgctg atggagctac gcgcgcagcg gcagcaggcg
     3301 gaagcggagc tgaaactggc gcgggtgacg tattcccgtc agcaacgtct ggcacaaacg
     3361 caggcggttt cactgcagga tctcgacacc gccgcgacgg agatggctgt gaaacaggcg
     3421 caaattggca ccattgacgc gcaaatcaag cgcaatcagg cttctctcga tacggctaaa
     3481 accaatctcg attacactcg catcgttgcc ccgatggccg gggaagtcac gcaaatcacc
     3541 actctgcaag gccagacggt gattgccgca caacaagcac cgaacattct gacgctggca
     3601 gatatgagca ccatgctggt aaaagcgcag gtttctgaag cggatgtaat ccacctgaag
     3661 ccggggcaaa aagcctggtt tacggtactt ggcgatccac tgacgcgcta cgaggggcaa
     3721 atcaaggatg tactaccgac gccggaaaag gttaacgacg ctattttcta ttacgcccgt
     3781 tttgaagtcc ccaaccccaa tggtttgctg cggctggata tgactgcgca agtgcatatt
     3841 cagctcaccg atgtgaaaaa tgtgctgacg atccctctgt cggcgttagg cgatccggtt
     3901 ggcgataatc gttataaagt caaattgttg cgtaatggtg aaacacgcga gcgtgaagtg
     3961 acgattggcg cacgtaacga taccgatgtt gagattgtca aagggctgga agcgggcgat
     4021 gaagtggtga ttggtgaggc caaaccagga gctgcacaat gacgcctttg ctcgaattaa
     4081 aggatattcg tcgcagctat cctgccggtg atgagcaggt tgaggtgctg aagggcatca
     4141 ccctcgatat ttatgccggt gagatggtag cgattgttgg cgcttcgggt tccggtaaat
     4201 cgaccctgat gaatattctc ggctgtctgg ataaggccac cagcggcacc tatcgcgtcg
     4261 ccggtcagga tgttgccacg ctggacgccg atgcgctggc gcaactgcgc cgcgagcatt
     4321 tcggctttat tttccagcgt taccatttgc tttcgcattt aaccgccgag cagaacgttg
     4381 aagtaccagc cgtctatgct ggtcttgagc ggaaacagcg gctgcttcgc gcccaggagt
     4441 tgctgcaacg actggggctg gaagaccgta cagagtatta tccggcacag ctttcgggtg
     4501 gtcagcaaca gcgcgttagc atcgcgcggg cattgatgaa cggcggtcag gtaattcttg
     4561 ccgatgaacc gaccggcgca ctggacagcc attcaggcga agaggtgatg gcgatcctgc
     4621 atcagctgcg cgatcgcggg catacggtga ttatcgtcac tcacgatccg caggtcgctg
     4681 ctcaggccga gcgggtgatc gaaattcgcg acggcgaaat tgtgcgcaat cctcccgcca
     4741 ttgaaaaagt gaatgttgct ggcgggacgg aacctgttgt caacacggcg tctggctggc
     4801 ggcagtttgt cagcggtttt aatgaggcgc tgacgatggc atggcgggcg ctggcagcga
     4861 ataaaatgcg tactttactg accatgctgg ggattataat cggtattgcg tcggtggttt
     4921 ccattgtcgt ggtgggcgac gccgccaaac aaatggtgct ggcggatatt cgttctattg
     4981 gtacgaatac tattgatgtc tatcccggga aagattttgg cgatgacgat ccgcaatatc
     5041 aacaggcgct gaagtacgat gacttaatcg ccatccaaaa acaaccgtgg gtcgcttcag
     5101 ccacacctgc cgtctcgcaa aacctgcgcc tgcgttataa caatgttgat gttgctgcca
     5161 gtgccaatgg cgtgagcggc gattatttta atgtctatgg catgaccttc agtgaaggaa
     5221 acacctttaa ccaggaacag ctgaacggtc gtgcgcaggt cgtggttctc gacagtaata
     5281 ctcgccgcca gcttttcccc cataaagcag atgtggttgg cgaggtgatt ctggtcggca
     5341 atatgcccgc cagagtcatt ggtgtggcgg aagaaaaaca gtcgatgttt ggtagcagta
     5401 aagtgctgcg tgtctggcta ccttacagca cgatgtccgg gcgagttatg ggccagtcgt
     5461 ggctgaactc cattactgtc agggtgaaag aaggatttga cagcgccgag gcggaacagc
     5521 aactcacgcg tttactttca ctgcgccacg gtaagaagga tttctttacc tggaacatgg
     5581 acggcgtctt gaaaaccgtt gaaaagacca cacgtacttt acaactgttt atgacgctgg
     5641 tggcggtgat ttcgctggta gtgggcggta ttggtgtaat gaatattatg ctggtgtcag
     5701 tgaccgagcg gacgcgggaa attggcattc gcatggctgt aggtgcgcga gcaagcgatg
     5761 ttttgcaaca gttcctgatc gaagccgtac tggtttgcct ggtcggtggc gcgttgggaa
     5821 taacactgtc actgttaatt gctttcacct tgcagctttt cttacccggc tgggagattg
     5881 gtttttcacc gttggcactg ctgctggcgt ttctctgctc gacggtcacc gggattttat
     5941 ttggctggtt acccgcacga aatgcggcac gactggatcc agtagatgct ctggcacgag
     6001 agtaattttt gagataaaaa tgccagccga tcgggctggc attttgcctt taggatgtac
     6061 acaatgagac agaagagcta tgcgactgcc gcttctactt cgacgggcac aataacactg
     6121 gcgtgattgc cttttggccc ctggtggaca tcaaactgaa cggattgtcc agcttttagc
     6181 gttctgtaac catccatctg aatggtggaa taatgagcga aaatatcttc gccgccgcct
     6241 tcagggcaga tgaaaccaaa ccctttggca ttgttgaacc acttaacagt acccttttcc
     6301 atgcttcgac atccttcgca aatcttatac aagtaagatg gaataaaccg gggtcagaga
     6361 gggggctgtt caaaacctcg ccaactctag aaatacaatt tagagaatta gggcgagccg
     6421 tcaagcattt gacaggggac agggggcagg tatgaatcaa aaatttgaag cagttaacgc
     6481 tattgacagg aatgtgacag atgtcgctga tgccaacgat agatgatagt tatttatcag
     6541 ttagtgttcg tgggtgttcg tcatggtgtg ggcatctaat ttatttctat gattataaag
     6601 gataatttcg tttttcttgt tcgcccttgt tcgtcacagt tcgcccttgt acgatgcacg
     6661 gtgtagtcaa tggtgtagtt aattttgatg accggaaaca cgaaacatga gcagggcaat
     6721 tcatagactt agcgacactc ttttacgaaa attaagcgga tcaccaacca caaaaagcac
     6781 cttttttaat gatggtggca accttagcgt aaggcattca accaatggcc tgttaacctg
     6841 gtatttcact tacaggtccg gaactggtag gcagatatca cctgaacgtc tgaggctggg
     6901 aaattatcca gatttgagcc tgaaagcagc cagggaaaaa gcgtcgcaat gtcgcgcatg
     6961 gctggctgag gggaaaaatc cgcgctatga acttaatcat gctgtacagg acgcgttagc
     7021 ccccgtgacg gttaaggaag cgctcactta ctggcttgaa tcgtacgcaa aggaaaagcg
     7081 cactgactac gaatcactga aaagccggat caataaacac ataattagcc agattggagc
     7141 tatgccactg gaaaaatgcg agctacgcca ctggctggcc tgttttgacc agcttgctaa
     7201 gcgtaatccg gtatcagccg gatttttgct acaggtatgc aaacaggcgc ttaagtactg
     7261 ccgaaaacga cgatacgcaa tcagcaacgt tcttgatgat atggttgtcg gtgatgttgg
     7321 gaaaaaagca gaaataagcg agcgcgtact aacaaacaag gagcttgggg aattactacg
     7381 cgcccttgat gaaaaaattt acccaccgta ctacagcgcc ctgattcgcc tcctgattgt
     7441 cttcggatgt cgtaccactg agctaagacg ctctgaggtc caggaatggg attttaaaga
     7501 aatgctctgg accgtgccga aagaacacag caaaacgaag gtagcaatat tcaggccaat
     7561 accggaagcg atcttgccgt ttgtcatgaa gctggtggag cagaacaggc acacgggctt
     7621 attgctcgga gaactgaaag ggcaatcatc cgtatcagag tacggaagaa cggcacacag
     7681 acgcattaat caagccccct ggacgttgca cgacatccgg cacacgttta caacgatgct
     7741 gaacgattta ggcgtggaac cgcacgttgt ggagcagctt accgcgcacc agttgccagg
     7801 aatgcaacga gtctataatc actcccgtta tcttgatgca aaacgtgacg ctcttaatct
     7861 atgggttgag cgtctcgagc ttctccagaa caatgataaa aaaatcgttg ttatgacccc
     7921 gcgaatttac tcccaaaatt cttgacaaat tatggctgtt tttcttgcga aaaatggccc
     7981 tctaaaataa atcgtcgaag ttcgaccaaa gttcgtcgtt tttttatttg tcaatcatcg
     8041 aaaaaattga aattcactct cctgaatcct tattctctgt ttcttgattg ttccaacacc
     8101 tgatggttta tctatttgtt tttaatgaga taatttaagt gtaataccaa atgacgacat
     8161 ttttttgttg cgtcgcttaa aaactcgtga cacgatttta tgaacagcag caaactctag
     8221 cgaactttga cgaacataaa gttcagaaag gttacgtatt caatttaaga ggtaagaaaa
     8281 gctaagaagt aatatctttc cgtatatcca cagcgaaaca aaaatttcta tacgtaattt
     8341 tatgtaggac tttttatgcg cgaaataaat gaagatcgtg taatccgcga aaaagaatgc
     8401 agagagctaa caggcgtttg ccgcacaact cgttacgaga tggaaaagca gggccgcttc
     8461 ccctcacgta tcagtctcgg cggtcgttct gttggctggg ttaagtctga ggttatggaa
     8521 tgggttaaaa gcagaaatcg tattaattca ggtaaggcag cgtaggggga acatatgaca
     8581 cataaaacaa aggcgacctg tggcggtcgc caatgggaaa acactaaaca taagccagaa
     8641 catcatagcg atttgctgtc tggtgggcaa tgtgctcagt gtgctggctt gctggtgggc
     8701 tattcctgct ctttggcctt gcgacgctgg cggcgtttga tttcgccgcg catggctgcg
     8761 tttataaact gccccgtgct ttcaccttct tctttcacgt tctccatgcc ttcaataatt
     8821 tcatgggcaa tacgtgcagt tacagattgt gattttgcat tctttggacc cgttgccatg
     8881 tttaacccct catgtttttg gtgtaagaca atatacacga aaaaaaaata ttttgaaggg
     8941 ttgacgtgta agacaccgtt attttacatt gtgtcttaca ccttgttgat gcaaggtaca
     9001 gaaaagacga aacccggcag tgcgtcaaca ctaaccgggc ttctaaccac caacgatagc
     9061 gaaagtatcg aggtagctat gagaaatcat atcacacacc cgcaagggcg ggactcgcac
     9121 aacctgaata aatacatctg gcgttttatc gccctgagca ctgcacaacc gcgcgtgatt
     9181 accattgagg ccaccagcga acaggaagca cgccagcaat ctcctgatgg ctgcgtgatg
     9241 gtattcgctg cccgtattcg ccagggagaa attttgtgat gcataaaatc ccttttgaag
     9301 tattaatcca ctctgaaaac gcattaatta aagcgcgcga aatgaatgcg ttgttaatta
     9361 aattaattga agtgccggaa ggtggtgagg aatcaggtgt attaatgttt gcagccattc
     9421 agacattact atcgcctgtt attgatgaac tggataaggc aatggcaatt cacgaaaata
     9481 ataacgtgcc ccacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
     9541 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
     9601 cacggtaatg attaagggcg tggcgggacg gtgcgtaact taccgccgtg aaggttacga
     9661 atatgattgc gtgatgcctg tttatcagtt ccggcgtgat ttttctctgg tacagaccgc
     9721 gccgcataac gtgcccacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
     9781 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
     9841 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtca
     9901 cacgcggaac aatggcagga gcaggttggc cccgcatccg gcgataatgt gccgcctgtg
     9961 tggcttgggc caaatcagct tgccgaactt gatgcgctga aaattgttcc ggatgggaaa
    10021 aagcgcgtaa ggctgtacca ggccggagaa ctggatttag tggagaccaa aaagattggt
    10081 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
    10141 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
    10201 cttaccatgc cggagcagaa aaatacgcaa ttggcacaaa tggcagacag tgagcgcgca
    10261 cagatgcttg ctggtcgatt tgatggcgtt tgtgtgcatc cggaaagtga aatcgttcac
    10321 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
    10381 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
    10441 gcgttaaaag ttattgccga accaatgggc gagccgaccg gcgatttgct gccgttcgcc
    10501 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
    10561 accacgcaca acggtattga gtacacgcaa ccagcaccag gggagaacat ccgcgataac
    10621 gcgccaaact ttcataaatg gcttgagcac gcagccggaa aagacccgcg caagatgatg
    10681 cggatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
    10741 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
    10801 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
    10861 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
    10921 ggaacgggca tcaagaaaat cacgggcggt gaccccgtgg aaattaaccc gaaatatgaa
    10981 aagcgtttta cggcggtaat cagggcggtg gtgctggcga ccaacaacaa cccgatgata
    11041 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
    11101 gtcagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
    11161 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
    11221 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
    11281 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
    11341 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
    11401 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagcctgcc
    11461 gcaaaagttt acggacatga atatattacg cggaaagtta agggggtaac gcagactaac
    11521 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
    11581 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agacagcctt
    11641 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
    11701 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc cgtgccatta
    11761 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
    11821 ttaaacgagg gggtaaaaat aaatatgtac acttcaggga aatttaataa atcactcaaa
    11881 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
    11941 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
    12001 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
    12061 gagattgcgg aagctgaaca ggtgacaacc atcaacgtta aaaacgtaga ggtggaatca
    12121 tgacagcaca gatagcggct tacgggcggc tggtggctga cccacagtta aagaccacca
    12181 gcaagggcac acaaatgacg atggcgagta tggcggttcc cctgacatgc agccaggctg
    12241 atgacggaac ggcgacgatg tggttatccg tcctggcgtt tggcagacag gccgacgcac
    12301 tggcaaaaca ccgcaaaggc gagctggtga gcgtggcggg taacatgcag gtaagccagt
    12361 ggacaggcca gaacggcgaa acgcggcagg gctggcaggt tatcgcagac agcgtaatca
    12421 gtgcgagaac ggcgcgaccg ggcggcaaaa aaggccagca ggggcaggct actgacgcac
    12481 tgaacagggc aaaacaacag gcggggaatg atgatccgta cggggataac ataccgtttt
    12541 aagcaacgag tggcagaagc cggagcaatc cggctttttt gt
//
LOCUS       DAZKFZ010000128        12572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000128 DAZKFZ010000000
VERSION     DAZKFZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.128"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(129..902)
                     /gene="uxuR"
                     /locus_tag="ACHOQW_003201"
     CDS             complement(129..902)
                     /gene="uxuR"
                     /locus_tag="ACHOQW_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP9574240.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1117..2577)
                     /locus_tag="ACHOQW_003202"
     CDS             complement(1117..2577)
                     /locus_tag="ACHOQW_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP9574241.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2658..3842)
                     /gene="uxuA"
                     /locus_tag="ACHOQW_003203"
     CDS             complement(2658..3842)
                     /gene="uxuA"
                     /locus_tag="ACHOQW_003203"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9574242.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            4182..5525
                     /gene="gntP"
                     /locus_tag="ACHOQW_003204"
     CDS             4182..5525
                     /gene="gntP"
                     /locus_tag="ACHOQW_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP9574243.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5699..6601)
                     /gene="fimH"
                     /locus_tag="ACHOQW_003205"
     CDS             complement(5699..6601)
                     /gene="fimH"
                     /locus_tag="ACHOQW_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9574244.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNQGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6621..7124)
                     /gene="fimG"
                     /locus_tag="ACHOQW_003206"
     CDS             complement(6621..7124)
                     /gene="fimG"
                     /locus_tag="ACHOQW_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFP9574245.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7137..7667)
                     /gene="fimF"
                     /locus_tag="ACHOQW_003207"
     CDS             complement(7137..7667)
                     /gene="fimF"
                     /locus_tag="ACHOQW_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP9574246.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7677..10313)
                     /gene="fimD"
                     /locus_tag="ACHOQW_003208"
     CDS             complement(7677..10313)
                     /gene="fimD"
                     /locus_tag="ACHOQW_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP9574247.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLARNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10380..11105)
                     /gene="fimC"
                     /locus_tag="ACHOQW_003209"
     CDS             complement(10380..11105)
                     /gene="fimC"
                     /locus_tag="ACHOQW_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9574248.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11142..11681)
                     /locus_tag="ACHOQW_003210"
     CDS             complement(11142..11681)
                     /locus_tag="ACHOQW_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9574249.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11746..12294)
                     /gene="fimA"
                     /locus_tag="ACHOQW_003211"
     CDS             complement(11746..12294)
                     /gene="fimA"
                     /locus_tag="ACHOQW_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9574250.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         2969 a         3292 c         3084 g         3227 t
ORIGIN      
        1 atgagtacta acattcaggt tatatagata tttaaacaat accctgcaat ttacaaagta
       61 atactcattg cgacttctta atttaagaaa gcgcaaggaa cgtttaccct tgcgcttatt
      121 ataataagtc aggcgtcgac tttatccagc ggccatgaat caaacagata gccgtcaaaa
      181 taaatatcgt caacgttcga gaattccagc agacgttgct taacgttttc cagatgctgc
      241 cacattgcca gcttcgccgc tcgggcatct tttttgatta acgcggcgag gatctgtttg
      301 tgatcgccca accactcttt gcgatacagg ctgtcatcca gatggctgtg caactggatc
      361 cacattgggt tgttttcccg ccactgccag gactgacgga acagctccac cagcatgctg
      421 ttatgcgttg cttcggcaat agcgagatgg aactgcatgt caccgctttc gctgctgccc
      481 ggcgcactgg aagccagttc acgctcttcc agttgcaatg cctgacgcat tttgacgata
      541 tcttcgcggg tagcctgcag ggcggcaaac tcggcgatgt tactttccag taactgacgc
      601 gcctgtaaca gctcaaaagg acccgcatca ttgcagacgt tggcatccgg actgtctgtg
      661 ttgtggctgc ctgagctatc aagaacatag ataccggcac cccggcgcac ttccaccagc
      721 cctttgatct ccagcatgat cagcgcttca cgtaccaccg tccgcgtgac atcaagcatt
      781 tctgcaattt cacgctccgg cggtagccgt tcaccaggat tgtacggcgt ctttatgatc
      841 agatcgcgga tcatcgcccc gacttcctgg taaggtcttt gcgcagaggt ggcagatttc
      901 atggtgttgt gtccgttagg tgcggttaat caaatttttg ccggatgcgg cgtgaacgcc
      961 ttatccggcc tacaacgggc aattttacgg cccccgttaa tctcaaacta taccattagc
     1021 actggaagtt ggcacgtaca ggtgggcaaa aaaacgaaaa ctggtcaacc aaatatcagt
     1081 ttgattgacc agtaagtctg tagggtaatc agttagttac agcgcagcca cacactcgcg
     1141 cgcaccgcgt tcgcacagct gctgatatgc cgcagtcacc gcgccaacaa agtcggcatt
     1201 ctgcggcaga tcatcggcaa aaataccgct caggcccagc agcgctttca cgcggtctgc
     1261 gccctgatac tgcgcgttaa tcttctggaa ctcggccagc atcgggtcga ccacgtcaat
     1321 ggcattaccc tgctcatcca cgccctgggt gtaacgcatc cagccagcca cgcccagcgc
     1381 caggtgacgc cagctgccgc cgttttgcag gtgcagacgc accgggtcca gcagacgctg
     1441 cggtaacttc tggctgccgt ccatcgcaat ctgccaggta cggtgacgca gagacgggtt
     1501 gctgaaacgc tcgatcagca gcgtcgcata ggcgttcagg tccgtacctt ccggcatcga
     1561 cagcgttggc gcttgttcct gcatcatcag ggcaaaggcc gctttgcgat aatccgggtt
     1621 agtcatggtg tcggcaatag tttcatagcc gccgaggtaa ccgaggtacg ccaggaaaga
     1681 gtggctgccg ttcagcatac gcagtttcat catttcgaac ggcacaacgt ctgcaacgaa
     1741 ctgtgcgccc actttatccc agtccggacg accattaacg aagttatctt caataaccca
     1801 ctgacggaac ggttcgcagg caatggcgca cgggtcgtaa acacccagct ggtcagcaat
     1861 ttcctgtaag gtttctggcg tcgccgccgg aacgatgcgg tcaaccatgg tgcacgggaa
     1921 ggtgacgttc tcttcaatcc atgccgctag ctgcgggtca cgcgcctgag ccagccccag
     1981 taccgcgacc ttcgccacat gaccgttttc acgcacgtta tcgcaggaca tcaccgtaaa
     2041 tgctttcagc cctttttcac gacgcagacg caaggcttcg acgatgtaac caatcgcgga
     2101 cttcggcgca gtcgggtttt ccagatcgtg cttgatcagc gggttattga gatccagttg
     2161 accgcttgcc gcatcagcgc agtagccttt ttccgtgacc gtcagggaga caatcgccgt
     2221 ttgcggacgc gccatcgcgt tgagaatacc ttcgcagccg tcgatttccg ggtgcagcgc
     2281 ttctttcatc gaaccgataa ttttcagctc ggtgctttcc gcgccttttt ccgccacggt
     2341 gtacagcagt tgctgttttt tcaggttttc gatcagcacg cggtcgttgc ctggcatcag
     2401 gttaacttcg cagatacccc agtcgctgtc ggtgctttcc agcagatgat gggtgtacag
     2461 cgcctggtgc gcacggtgaa acgccccgca accgagatgc acaatgcgtg attccagacg
     2521 agaatgatcc catgacgggc gggcaaccgg cagattgctg tcaacaatag tagtcatcgt
     2581 aaactccaga atccgtcttc agagagtgac ggcaaggaag aaggagccgg cacggcgcgc
     2641 agccatgccg gtgggtatta atggctaaag aaagcgcgct ggatcgccag ttcgacaccg
     2701 cgaacttcgg ccaggccttt cagacgacca attgcggagt aaccagggtt ggttttcttc
     2761 ttcaggtcgt ccagcatctg atgaccgtgg tccggacgca tcgggatcag gtcttctttg
     2821 ccttccgctt tacgacggtg ttcttcttca acaatcgctt tcaccacttc gtacatatca
     2881 acgtcaccgt tcaggtgcgc cgcttcgtgg aaggttttcg ggttatcttc acgcatggtg
     2941 gagcgcagat gggtgaagta aatacgcgga ccgaactgct tgatcatatc aaccaaatcg
     3001 ttgtcagcac gcacgccgta ggaaccggtg cacatggtga aaccgttcgc catgctgttt
     3061 acggtatcaa ccatccactg catatcttca atggtggaaa caatgcgcgg caggccgagg
     3121 atcgggcgcg gcggatcgtc cgggtgaaca gccatacgga cgccaacttc ttcggcaacc
     3181 ggaataatcg ctttcaggaa gacagcaaag ttttcgcgca gtttggcttt gtcgatatct
     3241 ttgtacagct ccaggtgctt acggaactgg tcgagggtgt acccttcttc cgcacctggc
     3301 agaccagcga tgatgttacg ggtcagacgc gctttgtctt catcgctcat agtggcgaag
     3361 cgttcagctg cctgagcaat ttcttcttcg gtgtaatccg cttccgcgcc tggacgtttc
     3421 aggatatgca tttcgaatgc agcgaattcg atctggtcga agcgcagagc tttggagccg
     3481 tctggcagca cgtattcgag gtcagtacgg gtccagtcga gcaccggcat gaagttgtag
     3541 cacacggtgc gaatgccgca ctgcgccagg ttgcgcagag tctgctgata gttagcgatc
     3601 cactgctcat agttgccagt gtgggttttg atatcttcgt gaattggtac gctttctacg
     3661 acagaccaca ccaggcctgc gtcttcaacg atcgccttgc gtttgaggat ctcttctacg
     3721 gaccatactt cgccgttcgg gatatggtgc agcgcggtaa ccacgccagt tgcgcccgcc
     3781 tgacggacat cagctaaaga aaccggatcg tttgggccgt accagcgcca ggtctgttcc
     3841 atgatttttc ctctttcaca tagtgggttc cacagcaaag agacgcggaa ccctggagtt
     3901 gtttaggtat ccggatgaac aaccagaccg cagccgtggc gttgcatcgg atgagatggc
     3961 gtataagttc taccttttgg taagacatgt caacccgaat tctgaaattg gttaaccaca
     4021 tcacaagaat ttcactctca tggttgttgc tgcaaattgc agcaaagggt aatcgatggt
     4081 tattgatcag aggatgtgac attcatcgca acaatggttg accaatttac ataacatgtc
     4141 gcccaaaata acaacggttc aaccaggtaa ggtaaaacgt tatgcatgtg cttaacattc
     4201 tctgggtggt attcggcatt ggtctgatgc tggtactgaa tttgaagttc aaaatcaatt
     4261 caatggtggc tttgttggtg gcggcgctgt ccgtcgggat gctggcgggc atggatttga
     4321 tgtcgctgct gcacaccatg aaagcgggct tcggcaacac gctgggggaa ctggctatca
     4381 tcgtggtgtt cggtgcggtc atcggtaaat tgatggtcga ctccggcgcg gctcaccaga
     4441 tagcgcatac gctgctggcg cgtctcggtc tgcgctatgt acagctgtcg gtgattatca
     4501 tcggcctgat ttttggtctg gcgatgttct atgaagtggc ctttatcatg ttagcgccgc
     4561 tggttattgt cattgccgcc gaagctaaaa ttccgttcct gaaactggcg atcccggcag
     4621 tagcagctgc cactaccgca cattcactgt tcccaccgca gccgggtccg gtggcgctgg
     4681 tgaatgctta tggcgcagat atggggatgg tttatatcta tggcgtactg gtgacgatcc
     4741 caagtgtaat ctgcgcaggt ctgatcctgc cgaagttcct cggcaatctt gagcgcccaa
     4801 cgccatcatt cctgaaagca gatcaaccgg tagatatgaa caatctgccc tctttcggcg
     4861 tttcgattct ggtgccgctg atcccggcga tcattatgat ctctaccacc atcgccaata
     4921 tctggctggt aaaagatacc cctgcctggg aagtggttaa ctttatcggt tcctcgccga
     4981 ttgcgatgtt tattgcgatg gtggttgcat tcgtactctt tggcaccgcg cgtggtcatg
     5041 acatgcagtg ggtgatgaac gcttttgaaa gcgcggtgaa gagtattgca atggtgattc
     5101 tgatcatcgg tgcgggtggc gtgctgaagc aaactatcat cgacaccggc attggcgaca
     5161 ccatcggcat gttgatgtcc cacggcaata tctcgcccta cattatggca tggctgatca
     5221 ctgtgctaat tcgtctggcg acgggtcagg gtgtcgtttc ggcgatgacc gccgccggga
     5281 ttatcagtgc tgcaatcctt gatccagcaa ctggtcagct ggttggcgtg aatccggcgc
     5341 tgctggtgct ggcgacggct gcgggttcca acaccctcac ccacattaac gatgcatctt
     5401 tctggttgtt caaaggttac tttgacctgt cggtaaaaga cacgttgaaa acctggggtc
     5461 tgctggagct ggtcaactcc gtggttgggc tgattattgt gctgattatt agcatggtag
     5521 cgtaaaagaa caccagagcc tgccaatggc aggctcagtt cggattgtcg gcaaagtgcg
     5581 ctttgtttat gccagatgcg acgctgacgc gtcttatctg gcctacaaag ggctaacgtg
     5641 caggttttta gcttcaggta atattgcgta cctgcattag caatgccctg tgatttcttt
     5701 attgataaac aaaagtcacg ccaataatcg attgcacatt ccctgcagtc acctgcccgc
     5761 cggtacgtgc gtaatttgcc gttaatccca gacttaccgc cgaagttcct actgctccta
     5821 acgataccgt gttattcgct ggaataatcg taccgttgcg cgtcaactgt acgccgacgc
     5881 cctgcgctgg tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca tctgcggttg
     5941 tgccggagag gtaatacccc tggttttggc ttttcgcaca ataaacggta agaggaattg
     6001 gcactgaacc agggtagtcc ggcagagtaa cggtgacatc acgagcagaa acatcgcagc
     6061 cgccagtagg cactaccaca tcattattgg cgtaaatatt ccacacaaac tggaaatcat
     6121 cgctgttata gttgttggtc tgtcgcaaaa taagcacggc aattaatgag ccagctttaa
     6181 tcgccacccc gcccgcactg ctcacaggcg tcaaataaag cgccaccggc cacggcttat
     6241 ccgttctcga attataaaca acccgcggcg tttcgctggt ggtcggaaat ggatagctac
     6301 tgccactata ttttacggtc ccggaaaaat tagataacac gccgccataa gccgagcctc
     6361 gttgcagtgt gacatagtct gtaatggttt ccggataatc gttatggcaa aagatttgcg
     6421 tcgaaagatc tacgaccagg ttttgcccca cattcacggc aggcgcaagg tttacataaa
     6481 cattagcgct gccaccgcca atagggatag cggtaccatt ggcggtttta caggcgaatg
     6541 accaggcatt taccgaccag cccatcagca gtacagcaaa cagggtaata actcgtttca
     6601 ttacaattat ctcttcgggt tcagctgtag gtataggtga tgctaatcac tgcctgaatg
     6661 gttccctgag tggctccgcc atttaccgtc aatgctctga cctgtaacgg gaagtgcgct
     6721 gattgtgagg aatcatccac ctgaactgtt ttggttgcgc cagtattcaa tgtgttgcca
     6781 ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc cctggttttt ataatatccg
     6841 gtactgtcgg ctgccccgct gaagctggca gtgacccttg acgttcccac cggacaatta
     6901 gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc cggcagacat cagactgaaa
     6961 gaataaagat cgccgagatc aaccgtggca ttggtggtgg aaactgtgca cggtttggcg
     7021 acgaccttac cgttcaccgt gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc
     7081 gccaatattg ccgccaataa atacccacgt ttgcgccatt tcatgagcat ctccagttac
     7141 tgatattcaa gagtgaaggt agccgtggca ttgatatgcc ccgcagtgac aggcacctgt
     7201 gtcgccatta gccgggcgta aaaattcaac gtatttggtt tacccggcgt cagggtcgtc
     7261 cacgaaatcg cggacgatgg agcattaagg ggtatctgat tttgctgctc attcagaagc
     7321 tgtattccca gtcccgaagc cgctgacacc gtattttcaa gtgcaagcag gttggcattg
     7381 tggctatctg caacgccggt aaacccaact tttacggcag aaacggcgtt accacagggt
     7441 gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc caatgttgtt aaattgcttc
     7501 gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt cagcggccac actacagccg
     7561 ttatctctga catagccgcg gatagtaatc gtgctatccg cagccaaagc gtgactcact
     7621 gccagccaca aaaaagcgca cagaagataa aaaggtttgt ttctcatcac gcccccttaa
     7681 cgacattcag ctgatagctg ggttaataac tgctgctgac tctctggtgg cagttgataa
     7741 ttggcgacac aatgagcatt ttcctcttct ccccatttca cctgaacttt tcccgctaga
     7801 ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa tgccgctact ctggctactc
     7861 tctgatgtca ccatcgcccc aaacggcagc ggcttattat tgtgggttag cgtcatgagc
     7921 agttttatcc caacgcgcgc tttaaactct gctcgcacga tcgccccacg agtgggaaca
     7981 acgttagcga ccgcgttatc taaatcgacg ttatcagcca gggtattggt atccagcgcc
     8041 actctatttt cccgatattc agtggcataa ggcagcacgg cataaccgcg ccagtcggta
     8101 cgcacccccg tctggttttc gacttttgca tcttttgcgc caggcgcttt aacaagcacc
     8161 accgtatcgt ttaacggctg ccccagcgtt acgccattgg catgagccag taccccaccg
     8221 ctgactccgt aatagagctg cttaatatca tcgctatggc tgtaaccgat attggcattg
     8281 ccgtaaccac cgcgataatt cagcgtggcg tagcctgtgc ttccgctatt accatcgcct
     8341 cccccggcat agccggtttg cacgctatag ctgaggttgt tgtcttccag caacgtaccg
     8401 tatacaccag ccagattggt catccgaccg ttgagatcgt gtgacatgct gtagctggca
     8461 ctggcatgtc gccactgaga tttactgtca gaacgcagcc agtggctgaa aggaatattg
     8521 acgttacgcg ctaacatctg atcacgtcct ttttgccagg cgtttttcgt caggctatag
     8581 ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc cagcctggaa ttgctcatcg
     8641 acattactcg ttccccaata agtttgatgg ctaccactca aatacagtgt tgatgagcgc
     8701 ccgagttgct gagtaacggt gagttgtaat ttcccgcgtt tgttataagc gaggttgtaa
     8761 tagtcggtga atttcggctt aacctgaata actccgtcct gtgtttcgat gttgtagcca
     8821 ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga ataacggtaa
     8881 cccactaact gaatattcgt gcctgactca ttgagcgatt tgttatagag aaaacgcacc
     8941 gattgtccgt catgctgact gtcatcggga agtgtggaat tagcctgagt catatccaca
     9001 gacagagcgc ccagtgcccc catatttttc ccgataccaa aattaaaagc acgataacga
     9061 tctgccagtt gcgttccacc atatattgtc cagccagctg gaaggccgtg gagtaatgta
     9121 ctttggaaaa agcggggttt ttcctgttgc gcatttccac tacggtattc tcctgccgta
     9181 atggaataac gagtatgccc ttcacgttgc aaaagcggga ctgacgaata gggtacggta
     9241 aaaatctgcg tgctgccgtc agcctcttta atcgttacct gcaagtcacc actattacct
     9301 gcggcataga tatcgttgat ggtaaaaggc cccggcggca ccgtactatt ataaatgtca
     9361 tacccatttt gtttaatagt gacctgtgca gtaccacgag caataccgtg gatcaccggg
     9421 gcaaatcctc tttggctatc gggtaacata ttgtcatctg aggccaattg tgcgccgcga
     9481 aagttaatac catcgaaaat atcaccctga gtataaccat cacccagcgt cagccgggaa
     9541 cgtaacggta ttatgtctcg ctcaagccag gtattgatat gctgccattt atttttgcta
     9601 cctgatgatc tgtcgctact gttataactc caggtggtat tgtcgcgtaa acgccacgca
     9661 ccaatattta acccactctg taggtttaaa tatgcataat ggctgttacc cccaatccga
     9721 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
     9781 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
     9841 aggttcagtc gctgctgacc aacatctaaa tgcgcagtag cgtcctggac cattgtggtt
     9901 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
     9961 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
    10021 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
    10081 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
    10141 ggatcatccg ctaaaaagcg cggattaaaa tagagttcgg cagatgacaa aggtgcctgt
    10201 acggcaaaag cacaggcgac aaagagccgg acaaaaaaac cagccaaacg atgcttacga
    10261 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
    10321 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
    10381 tattccatta cgcccgtcat tttgggggta agtgcaccat aatcatttat tgttcggtaa
    10441 gtaatattgc ttcccgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
    10501 aaggcatttt caagaacccg ggctccggca ttcaactctg ttaccgtcag gtaatagggt
    10561 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
    10621 tgatcgggtg gcaacgctaa tttagccgga cgatagtaca gtttaatgcg gctgataatt
    10681 gcgagctgta gcgtattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
    10741 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
    10801 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
    10861 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
    10921 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tatgcgagtc
    10981 gcacctaagg ccactcccgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
    11041 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
    11101 ctcactgtcc tgttcctgtt atcacattat ctgctgaaca attactgata ggttaaagag
    11161 aaccaggcct gtgcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
    11221 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
    11281 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta cgccaatatt ggtggctatc
    11341 cctggcccct ctcccacgga aagcacatcc ggatttttac catccgcgac accgtgaaac
    11401 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
    11461 accggtgcgc tatcttcccc taccgcatga aaccggttac tgctgatttg ccccatattg
    11521 accgtcattt gtttatcacc ggcttcaatc aggcaagttt ccgcaataat gacgccctga
    11581 aattgcatat ttccgccggg cggcgtggta ttccatttat ttccggccag agcaaacatt
    11641 ggcagcaacg acgctaatag aaataatctc ttccttttca tcgttatttt tatcgcacaa
    11701 gggtgggcat ccctgcccgt aatgacgtcc ctgaacctgg gtaggttatt gatactgaac
    11761 cttgaaggtc gcatccgcat tagcagcacc cggggttgcc tcgccgattg cataataacg
    11821 cgcctggaac ggaatggtgt tagtaccgtt attcagggtt gtttgctcac tgaatgtcgc
    11881 accatccagc gtcagcgcag cacccgttct gtccaggatc tgcacaccaa cgtttgttgc
    11941 gctacccgca gctgaactct gcagagccag aacgttggta tgacccgcat caatcgccgt
    12001 acctaaaaag gcaacagcgg ctttagatgc aacattggta tcgcaatcat tcagctgaat
    12061 gttaaaaccg acagcagaac tggttgctcc gtcctgtgcc agcgatgcgg tacgaacctg
    12121 tcctaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
    12181 ttccccttta aagtgaacgg tcccaccatt aaccgtcgtg gcagcggcca gagccgctgt
    12241 agaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
    12301 ttcctttcaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
    12361 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
    12421 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgctggatta
    12481 atattctatt gttatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
    12541 aagaatgagg aagatgcgtc gagccacaga aa
//
LOCUS       DAZKFZ010000129        12181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000129 DAZKFZ010000000
VERSION     DAZKFZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..868
                     /gene="yjeJ"
                     /locus_tag="ACHOQW_003212"
     CDS             <1..868
                     /gene="yjeJ"
                     /locus_tag="ACHOQW_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9574251.1"
                     /translation="AISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRD
                     LLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTL
                     ELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSPL
                     DFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTR
                     EATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHLR
                     VQSTSKTAPQAK"
     gene            complement(1060..1413)
                     /gene="yjeI"
                     /locus_tag="ACHOQW_003213"
     CDS             complement(1060..1413)
                     /gene="yjeI"
                     /locus_tag="ACHOQW_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9574252.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1551..3197)
                     /gene="groL"
                     /locus_tag="ACHOQW_003214"
     CDS             complement(1551..3197)
                     /gene="groL"
                     /locus_tag="ACHOQW_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9574253.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3241..3534)
                     /gene="groS"
                     /locus_tag="ACHOQW_003215"
     CDS             complement(3241..3534)
                     /gene="groS"
                     /locus_tag="ACHOQW_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9574254.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3810..5066
                     /gene="yjeH"
                     /locus_tag="ACHOQW_003216"
     CDS             3810..5066
                     /gene="yjeH"
                     /locus_tag="ACHOQW_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9574255.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5082..5558)
                     /gene="fxsA"
                     /locus_tag="ACHOQW_003217"
     CDS             complement(5082..5558)
                     /gene="fxsA"
                     /locus_tag="ACHOQW_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9574256.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5895..7331
                     /gene="aspA"
                     /locus_tag="ACHOQW_003218"
     CDS             5895..7331
                     /gene="aspA"
                     /locus_tag="ACHOQW_003218"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9574257.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7449..8750
                     /gene="dcuA"
                     /locus_tag="ACHOQW_003219"
     CDS             7449..8750
                     /gene="dcuA"
                     /locus_tag="ACHOQW_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP9574258.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8866..9204
                     /gene="cutA"
                     /locus_tag="ACHOQW_003220"
     CDS             8866..9204
                     /gene="cutA"
                     /locus_tag="ACHOQW_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP9574259.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9180..10877
                     /gene="dsbD"
                     /locus_tag="ACHOQW_003221"
     CDS             9180..10877
                     /gene="dsbD"
                     /locus_tag="ACHOQW_003221"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP9574260.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10914..11489
                     /gene="yjdC"
                     /locus_tag="ACHOQW_003222"
     CDS             10914..11489
                     /gene="yjdC"
                     /locus_tag="ACHOQW_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9574261.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11596..11671
                     /locus_tag="ACHOQW_003223"
     tRNA            11596..11671
                     /locus_tag="ACHOQW_003223"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11629..11631,aa:Phe,seq:gaa)
     gene            11869..12028
                     /locus_tag="ACHOQW_003224"
                     /pseudo
     CDS             11869..12028
                     /locus_tag="ACHOQW_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2932 a         3146 c         2995 g         3108 t
ORIGIN      
        1 ggccataagc atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc
       61 actggcgctg aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgtcat
      121 ggaactgcgc gacctgctga ttgcgctgga aagtcgtctg catcaaaagc ataaactgga
      181 tgctgctgca cgccttcagt atgagcaagc tcgcgataaa gtcattaaga aaatggcaga
      241 aaacatccca gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa
      301 cacgctggaa cttaccgata atcaaggcga aaatcttacc ttcgtattaa cgctacatga
      361 cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat
      421 cattcatgca attaataatg cggaaatgcg tgaactggca ttacgtatta cctcaccgtt
      481 agatttcctg ccgttatatg acgttgactg ccagcaaaat ggcaatctgg aatatgacac
      541 ctattcccaa ccggaatgga aacataactt gttcgaccat tatctggcgg tgctttaccg
      601 ctttaaagat gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc
      661 aacgccgggt aaagaaatag aggcgatcac tcgccgaatg cttgatttca gcccacgact
      721 gaaaaaactc gtgggtgtgc catgtcaggt ctatgttcgc accgtagcgg cgaataacgc
      781 ccaaccgcta acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac
      841 cagcaaaact gcaccacagg cgaaataatc tcatctttgc taatgcgagg ctggagccat
      901 ccagcctgaa atgactgata acgtgatcgt tggtcatgcc ggatgcggcg taaacgcctt
      961 atccggccta caaattctca agcattcaat cagttgcaat gccttatgta ggcctgatag
     1021 cgaagcgcat ctggcaatta tacgtttgtc accatcacat cagttcgggc acttatatac
     1081 ctggccgata atctggctat ccgtcgggac aaaactggac aacattccct gcgacgggct
     1141 actgatgcca taaatcacgt taccgcccat tgcagccgcc tggttgcgca gatcgtttgc
     1201 tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg gaaagccagt tgctttgctt
     1261 acctgtcgca gtaccaatca gctggcactc tgcgcctggc tgctcgtcca caatgcgtac
     1321 actctgaccg gcagcactca attcgttgct ggagctacaa cccgccatca gtagcgcggc
     1381 accgacaatc cctgctaagt attttacgtg catgttattc cccataatca atgagctgga
     1441 cgccactcgc gtcgtccgtg tctgaatctt atactagaaa gatgaccaaa agaaaaaccc
     1501 ccagacattt ctgcccgggg gtttgtttat ttctgcgagg tgcagggcaa ttacatcatg
     1561 ccgcccatgc cacccatgcc acccatgccg ccagcagcgc ctaagtcagc tgcatcgttt
     1621 ttcggcaggt cggtaaccat gcattcggtg gtgatcatca ggccagccac agaagccgcg
     1681 tactgcagag cagaacgggt tactttggtt gggtccagga tacccatgtc gatcatgttg
     1741 ccgtattctt cggttgctgc gttgtaaccg tagttgccgt cgccgccttt aacggtgtta
     1801 gcaacaacag acggttcttc gccgcagttc aggacgatct gacgcagcgg agcttccatt
     1861 gcacgcagtg caactttgat acccacgttc tggtcttcgt tctgaccacg caggtcagcc
     1921 agtttagacg ctacgcggat cagcgcaaca ccaccaccag caaccacgcc ttcttctacc
     1981 gcagcacggg tcgcgtgcag ggcgtcttca acgcgtgctt ttttctcttt catttcaact
     2041 tcggtagcag cacctacttt gataactgca acgccgcctg ccagtttcgc tacgcgctcc
     2101 tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa tctgctgacg gatctgagca
     2161 acacggccct ggattgcagc ttcttcgccc acgccatcga tgatggtggt ggtgtctttg
     2221 ttgatcacaa cgcgtttagc ctgacccagg tcttccaggg ttgctttttc cagctccata
     2281 ccgatctctt cagagattac ggtaccgcca gtcagggttg cgatatcctg cagcatagct
     2341 ttacgacgat cgccgaagcc cggagcttta actgcagcaa ctttcacgat gccacgcatg
     2401 gtgttaacaa ccagagttgc cagcgcttcg ccttctacat cttcagcgat gatcagcagc
     2461 ggtttgcctg ctttggcaac ggcttccaga accggcagca tttcgcggat gttggagatt
     2521 ttcttgtcag ccagcaggat gaacgggctt tccagttcta ctgcgccagt ttccggcttg
     2581 ttgatgaagt aaggagacag gtagccacgg tcgaactgca taccttcaac cacgtccagt
     2641 tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc cttctttacc gactttgtcc
     2701 atcgcttcag cgatcagttt acctacggtt tcgtcggagt tagcggagat agtaccaacc
     2761 tgagcaatcg ctttagagtc agagcacggt acggacagcg ctttcagttc ttcaactgca
     2821 gcggtaacag ctttgtcgat accacgtttc aggtccatcg ggttcatgcc cgcagcaaca
     2881 gctttcagac cttcagtgat gatagcctga gccagtacgg ttgcagtggt ggtaccgtcg
     2941 cctgcagcgt cgttcgcttt agaggcaact tctttcacca tctgcgcacc catgttttcg
     3001 aacttgtctt ccagttcgat ttcacgagca acggaaacac catctttggt gatggtcggt
     3061 gcaccgaaag atttatccag aactacgtta cggcctttcg gaccgagggt aactttcact
     3121 gcatctgcca gtacgtttac gccgcgcagc attttcacac gagcgtcgtt accgaatttt
     3181 acgtctttag ctgccattat ctttattcct taaattcgta tgttcagtgt cgtgcgagga
     3241 ttacgcttca acaattgcca gaatgtcgct ttcggacatg atcaacactt cttcattgtc
     3301 gatcttctcg gatttcacac cgtagccatc gttgaaaata acgatgtcgc caactttcac
     3361 atccagcggc ttcacttcgc cattttcaag gatacggcca ttgccgacag ccagcacttc
     3421 gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg atgccgccag cagatttagt
     3481 ttcaacttct ttacgcttga cgatcacgcg atcatgcaat ggacgaatat tcattgataa
     3541 ctctcctttg agaaagtccg tatctgttat gggtgacgcc ggagcttacg tggtttcccg
     3601 gctggtgacc agagaaatgg ggatgaggct tcgccccttc aagggggaaa aagaaaaaaa
     3661 attctgatca gcacaaaatc gggtgaaaac cctgattcac ctcacatttc atcgcaattt
     3721 cttcatcgcc gtcataagcg aatctgattg tgctaccatc gaaaatctac gcagttgccc
     3781 aaaatttggg cgcaatcgga catcaaccca tgagtggact caaacaagaa ctggggctgg
     3841 cccagggcat tggcctgcta tcgacgtcat tattaggcac tggcgtgttt gccgttcctg
     3901 cgttagctgc actggtagcg ggcaataaca gcctgtgggc gtggcccatt ttgattatct
     3961 tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg ccactatccc agcgcaggcg
     4021 gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct tgagcgagtc accggctggc
     4081 tgtttttatc ggtcattccc gtgggtttgc ctgccgcgct acaaattgct gccggattcg
     4141 gccaggcaat gtttggctgg catagctggc aactgttgtt ggcagaactc ggtacgctgg
     4201 cgttggtgtg gtatatcggt actcgcggtg ccagttccag tgcaaatcta caaacagtta
     4261 ttgccggact tatcgtcgca ctgattgtcg ctatctggtg ggcgggcgat atcaaacctg
     4321 cgagtatccc cttccccgcg ccaggaaata tcgaacttac cgggttattt gctgcgttat
     4381 cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc ccatcttgcc tcggaattta
     4441 aaaatccaga acgtgatttt cctcgtgctt tgatgattgg tctgctgctg gcaggattag
     4501 tctactgggg ctgtacggta gtcgtcttac acttcgacgc ctatggtgaa caaatggccg
     4561 cggcagcatc gcttccaaaa attgtagtac aactgttcgg tgtaggagcg ttatggattg
     4621 cctgcgtaat tggctatctg gcctgctttg ccagtctcaa catttatata cagagcttcg
     4681 cccgcctggt ctggtcgcag gcgcaacata atcctgacca ctacctggca cgcctctctt
     4741 ctcgccatat cccgaataat gccctcaatg cggtgctcgg ctgctgcgtg gtgagcacgt
     4801 tggtgattca tgctttagag atcaatctgg acgctcttat tatttatgcc aatggcatct
     4861 ttattatgat ttatctgtta tgcatgctgg caggctgtaa attattgcaa ggtcgttatc
     4921 gactactggc ggtggttggc gggctgttat gcgttctgtt actggcaatg gtcggctgga
     4981 aaagtctcta tgcgctgatc atgctggcgg ggttatggct gtttctgcca aaacgaaaaa
     5041 cgccggaaaa tggcataacc acataatccg gcgtttcgac attaatcctg gcgatcgtct
     5101 ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctggt actcaccatc aaaagtatta
     5161 ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc gagaaaagcg caaatgcggc
     5221 atcaacttca ctgtcagatg cttttgcacc ggcggcaata aaagcagaag accgaggaag
     5281 tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa tgatcagcga aacactttta
     5341 atcatctccg ccgctgggtt ttcacccgcc gccatttttt gctgcatcag cacgaaattc
     5401 ttaaagccct ggttacgtac cagtgacata ccgataactg acgtaaatat aaccagcacg
     5461 agagtcagca atacccccaa tacatgggca acctgaataa agattgaaat ctcaatatag
     5521 acataaagga aaatggcaat aaaaggtaac cagcgcaaag gtttctcctg taatagcagc
     5581 cggttaaacc cggctacctg aatgggttgc gaatcgcgtt cagcttatat tgtggtcatt
     5641 agcaaaattt caagatattt gcgcaactat ttttggtagt aatcccaaag cggtgatcta
     5701 tttcacaaat taataattaa ggggtaaaaa ccgacactta aagtgatcca gattacggta
     5761 gaaatcctca agcagcatat gatctcggat attcggtcga tgcaggggat aatcgtcggt
     5821 cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa gcaaatcatt ggcagcttga
     5881 aaaagaaggt tcacatgtca aacaacattc gtatcgaaga agatctgttg ggtaccaggg
     5941 aagttccagc tgatgcctac tatggtgttc acactctgag agcgattgaa aacttctata
     6001 tcagcaacaa caaaatcagt gatattcctg aatttgttcg cggtatggta atggttaaaa
     6061 aagccgcagc tatggcaaac aaagagctgc aaaccattcc taaaagtgta gcgaatgcca
     6121 tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg catggatcag ttcccggtag
     6181 acgtctacca gggcggcgca ggtacttccg taaacatgaa caccaacgaa gtgctggcca
     6241 atatcggtct ggaactgatg ggtcaccaga aaggtgaata tcagtacctg aacccgaacg
     6301 accatgttaa caaatgtcag tccactaacg acgcctaccc gaccggtttc cgtatcgcag
     6361 tttactcttc cctgattaag ctggtagatg cgattaacca attgcgtgaa ggctttgaac
     6421 gtaaagcggt cgaattccag gacatcctga aaatgggtcg tacccagctg caggacgcag
     6481 taccgatgac cctcggtcag gaattccgcg ctttcagcat cctgctgaaa gaagaagtga
     6541 aaaacatcca acgtaccgct gaactgctgc tggaagttaa ccttggcgca acagcaatcg
     6601 gtactggtct gaacacgccg aaagagtact ctccgctggc agtgaaaaaa ctggctgaag
     6661 ttactggctt cccatgcgta ccggctgaag acctgatcga agcgacctct gactgcggcg
     6721 cttatgttat ggttcacgga gcgctgaaac gcctggctgt gaagatgtcc aaaatctgta
     6781 acgacctgcg cttgctctct tctggtccac gtgccggcct gaacgagatc aacctgccgg
     6841 aactgcaggc gggctcttcc atcatgccag ctaaagtaaa cccggttgtt ccggaagtgg
     6901 ttaaccaggt atgcttcaaa gtcatcggta acgacaccac tgttaccatg gcagcagaag
     6961 caggtcagct gcagttgaac gttatggagc cggtcattgg ccaggctatg ttcgaatccg
     7021 ttcacattct gaccaacgct tgctacaacc tgctggaaaa atgcattaac ggcatcactg
     7081 ctaacaaaga agtgtgcgaa ggttacgttt acaactctat cggtatcgtt acttacctga
     7141 acccgttcat cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc gaaaccggta
     7201 agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa cttgacgata
     7261 ttttctccgt acagaatctg atgcacccgg cttacaaagc aaaacgctat actgatgaaa
     7321 gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc acgtcagatg acgtgccttt
     7381 tttcttgtga gcagtaactt aaaaataaca atctaatatc aacttgttaa aaaacaagga
     7441 aggctaatat gctagttgta gaactcatca tagttttgct ggcgatcttc ttgggcgcca
     7501 gattgggggg aataggtatt ggttttgcag gcggattggg ggtgctggtt cttgccgcta
     7561 ttggcgttaa acccggtaac atcccgttcg atgtcatttc cattatcatg gcggttatcg
     7621 ccgctatttc tgccatgcag gttgctggcg gtctggacta tctggttcat cagacagaaa
     7681 agctgctgcg ccgtaacccg aaatacatca cgatcctcgc accgatcgtg acctatttcc
     7741 tgactatctt tgctggtact ggcaacatct ctctggcgac actgccagtt atcgctgaag
     7801 ttgcgaagga acaaggcgtt aaaccttgcc gtccgctgtc tactgcagtg gtatccgcgc
     7861 agattgcgat caccgcatcg ccaatctcag cggcagtggt ttacatgtct tccgtgatgg
     7921 aaggtcatgg catcagctac ctccatctgc tctccgtggt catcccgtcc accctgctgg
     7981 cggttctggt gatgtccttc ctggtcacta tgctgttcaa ctccaaactc tctgacgatc
     8041 cgatttatcg caagcgtctg gaagagggcc tggttgaact gcgcggtgaa aagcagattg
     8101 aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct gctgggcgta gttggcgtgg
     8161 ttatctatgc aatcatcaac agcccaagca tgggtctggt tgaaaaaccg ctgatgaaca
     8221 ccaccaacgc aatcctgatc atcatgctca gtgttgcaac tctgaccacc gttatctgta
     8281 aagtcgatac cgacaacatc ctcaactcca gcaccttcaa agcaggtatg agcgcctgta
     8341 tttgtatcct gggtgttgcg tggctgggcg atactttcgt ttccaacaac atcgactgga
     8401 tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg gctgctggcc gtcatcttct
     8461 tctttgcttc tgctctgctg tactctcagg ctgcaaccgc aaaagcactg atgccgatgg
     8521 ctctggcact gaacgtttct ccgctgaccg ctgttgcttc tttcgctgcg gtgtctggtc
     8581 tgttcattct gccgacctac ccgacgctgg ttgctgcggt acagatggat gacacgggta
     8641 ctacccgtat cggtaaattc gtcttcaacc atccgttctt catcccgggt actctgggtg
     8701 ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt catgctgtaa tgaccaatcg
     8761 cggggcgttc acgccccgct ttctttctcg ccgactaaca tcctttcccc gtccgttgta
     8821 tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg tgtttatgct tgatgaaaaa
     8881 agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac cggatgaagc gacagcccag
     8941 gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct gcgcgacctt gatccccggc
     9001 gctacctctc tctattactg ggaaggtaag ctggagcaag aatacgaagt gcagatgatt
     9061 ttaaaaacta ccgtatctca ccagcaggca ctgctggaat gcctgaagtc tcatcatcca
     9121 tatcaaaccc cggaacttct ggttttacct gttacacacg gagacacaga ttacctctca
     9181 tggctcaacg catctttacg ctgatcctgc tactttgcag cacttccgtt tttgccggat
     9241 tattcgacgc gccgggacgt tcacaatttg tccccgtgga tcaagccttt gcttttgatt
     9301 ttcagcaaaa ccaacatgac cttaatctga cctggcagat caaagacggt tactacctct
     9361 accgtaaaca gatccgcatt acgccggaac acgcgaaaat tgccgacgtg cagctgccgc
     9421 aaggcgtctg gcatgaagat gagttttacg gcaaaagcga gatttaccgc gatcggctga
     9481 cgcttcccgt aaccatcaac caggcgagtg cgggagcgac gttaactgtc acctaccagg
     9541 gctgtgctga tgccggtttc tgttatccgc cagaaaccaa aaccgttccg ttaagcgaag
     9601 tggtcgccaa caacgcagcg tcacagcctg tgtctgttcc gcagcaagag cagcccaccg
     9661 cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg tattggtatc gcctttacgc
     9721 catgcgtgct gccaatgtac ccactgattt ctggcatcgt gctgggtggt aaacagcggc
     9781 tctccactgc cagagcattg ttgctgacct ttatttatgt gcaggggatg gcactgactt
     9841 acacggcgct gggtctggta gttgccgccg cagggttaca gttccaggcg gcgctacagc
     9901 acccatacgt gctcattggc ctcgccatcg tctttacctt gctggcgatg tcaatgtttg
     9961 gcttgcttac tctacaactc ccctcctcgc tgcaaacgcg cctcacgctg atgagcaatc
    10021 gccaacaggg cggctcaccc ggcggagtgt ttattatggg gacgattgcc ggactgatct
    10081 gttcaccatg caccaccgca ccgcttagcg cgattctgct gtatatcgcc caaagcggga
    10141 acatgtggct gggcggcggc acgctttatc tctatgcgtt gggcatgggc ctgccgctga
    10201 tgctaattac cgtctttggt aaccgcttgc tgccgaaaag cggcccgtgg atggaacaag
    10261 tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt cttcctgctg gagcgagtga
    10321 ttggtgatgt atggggatta cgcttgtggt cggcgctggg tgtcgcattc tttggctggg
    10381 cctttatcac cagcctacag gccaaacgcg gctggatgcg cgtggtgcaa ataatcctgc
    10441 ttgcagcggc attggttagc gtgcgcccac ttcaggattg ggcatttggt gcgacgcata
    10501 ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa aacggtagat gagttaaatc
    10561 aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga tctttatgcc gactggtgcg
    10621 tcgcctgtaa agagtttgag aaatacacct tcagcgaccc gcaggtgcaa aaagcgttag
    10681 cagacacggt attacttcag gccaacgtca ctgccaacga cgcacaagat gtggcgctgt
    10741 taaagcatct taatgtcctt ggcctaccga caattctctt ttttgacgga caaggccagg
    10801 agcatccaca agcacgcgtc acgggcttta tggatgctga aaccttcagc gcacatttgc
    10861 gcgatcgcca accgtgaacg acacttgcag tgggaaagac ggaggaaaat agcgtgcaac
    10921 gtgaagatgt actgggagaa gccctgaaat tattagaatt acaagggatt gccaacacca
    10981 cgctggagat ggttgctgaa cgtgtggatt atccactgga cgagctacgc cgcttctggc
    11041 cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct tagccaacag atagatgtct
    11101 ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga acaaaagctg ctggcacgtt
    11161 atcaggcgtt atcggagtgc gttaaaaaca accgctatcc gggctgtctg tttatcgctg
    11221 cctgtacgtt ttatcccgat cctggccacc ctattcatca actggccgat cagcaaaaaa
    11281 gcgcggccta cgatttcacc cacgaactgt taaccacgct ggaagttgac gatccggcga
    11341 tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt aagccgaatg ctggtgaatc
    11401 gtagccaggc ggatgtcgac accgcacatc ggctggcgga agatatcctg cgcttcgccc
    11461 gctgccgcca gggtggtgca ctgacctgac agaaacacag aaaagaagcg atttgccgca
    11521 atcttaagca gttgaatcgc ttttactgaa attaggttga cgagatgtgc agattacggt
    11581 ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc aggggattga aaatccccgt
    11641 gtccttggtt caattccgag tccgggcacc aaattcatat aaacggacct ccacggaggt
    11701 ccgtttttcg tttcaggacg ccacgattta agtgttctgc ctccagatca attctaccga
    11761 actcaacccg attctcccca catcaccagc aatttgcggg catatcccaa ttcgggaaaa
    11821 tttgttcctg agctatagca ctaactaacg tgaaatgtcg tgcggccccg tgatgctgtt
    11881 gaacgtcaaa tgacgtcatc aggagcgtaa cgcatccata aagcgcaaca tcggtctgaa
    11941 cgccgactga tgagattttc tgaatgagaa caaagagaaa tgtatcagtc cgcttgctca
    12001 tgcaaagact aacaatccat taaaatagta agcgctccgg acaattttcc atggattatt
    12061 ttctgaacat ttttctttgg caaagatgat gaattttgat ggtaaggaat attacttctg
    12121 gttctcagta aaatcctttc gtaataccat gtaatcaaga agtttatggc tggtaaaaat
    12181 a
//
LOCUS       DAZKFZ010000130        12062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000130 DAZKFZ010000000
VERSION     DAZKFZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(177..551)
                     /locus_tag="ACHOQW_003225"
     CDS             complement(177..551)
                     /locus_tag="ACHOQW_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574262.1"
                     /translation="MTLFDECREALSADFNIVEGLAQQEALGILNKYPLAKGSVTWAE
                     IWHSDYESFDELLCANSVKNDDMFVFADDASIPVFRSNLRLIAENIYDVTALSPKLFI
                     FNDEVIIQPLFPTDMFRLGIKK"
     gene            complement(557..7783)
                     /locus_tag="ACHOQW_003226"
     CDS             complement(557..7783)
                     /locus_tag="ACHOQW_003226"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFP9574263.1"
                     /translation="MESAGRLDISAGSLNNHQGTVVSDGLSVTLDGALDNTSGRLLSQ
                     KTLSVSGSELVSDDGLIQSGSDMTLDVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNN
                     QQGFMVGQKDMTLNAGTLDNRQGVLGSQASLQISSGTLMNQKGALKAGTDMLLSGGDV
                     SNQEGTLAAGRDLNAHLNVLENQQGTVVSNGNSRLDVTRSDNQGGRLVAQQSLTLSST
                     DIINDASGLIQSGASLNLRADTLSNRNSGDRGGVISQGSMTLNAGTLDSTAGVLLSGD
                     ALSLTAGVVNNTSGQVVANGLLGWNSQALNNQSGLIQGKGISINTAGQTLDNRGGTLN
                     SLQELTVSTGAMDNRSGTVGAKTTADLSTTSLDNREGGRLVSEGELRLHTGGLQNSHG
                     QIQSVGDILFDSVRGVVDNVSGLIRSGSAITLNALQFINRHTQNTGQGLEAQTIHITT
                     QDLDNQEGSILADRALTVMADRTLSNNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQS
                     VSVDAGQLGGDGKLLSLGDMTLKSNTTFSNSGQTIANGNLTLSVNGDVSNTGSLLAGS
                     RLDLNSIRLENTEKGEISAGQTWLNVTDTLLNRGLIDGKYTHLQANTLTNSGTGRIYG
                     DAVGVSAATFNNLDENGVAAVLAGRERVDLGVQTLNNRTHSLIYSAGDMHTGGMLDAN
                     GAATGKAGVLNNHSATIEAAGYLVLSAGQINNVNDHFTTERVVVSTEKVTEYQLSGSD
                     KRWSAGEPGVYVDNDSSNSLKKLHTPEGARDKFTQYDYTRTVEETRVKESDPGKILSG
                     AGMTIVADKLFNDKSQVVAGGKLTIPSGNVENVSVSGEQHVTDKGTSTYYYRIKKKGT
                     DKQGESISPYEPPTAIQTITLKPGELTSHGQVQGSQVTLSPLKPQGTDVQTGLTGNVD
                     ATVAGTDRIPLRPVVSAGEPVILLPGQQFEVSAPQGSIHVAGPDTRLPDSSLFKTNPA
                     VNVPYLVETDPRFTNQKTWLGSDYMQKAFSQNGDNMLKRLGDGFYEQRLIREQVVALT
                     GQRYLDGYSNDEEQFKALMDAGIAFGKQYNLTPGVALTAEQMALLTGDIVWLVNTTVT
                     LPDGSTQTVQVPQVYARVKPGDVNSAGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLS
                     AENIHNQAGTIQGANVSLTARTDINSTGGLLQATDSLLAMAGRDINLTTTTRTAQSDA
                     GQNHFERTSIDSVAGVYVQNDQGRLVLQAGRDMNLTAATVVNQGKDSLTQLSAGRDMT
                     LSTVTTSAQDNITWDKNNRLSQGVTQSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKG
                     LALMAGHDVTLTGAQNTSSLDEYHKVTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDT
                     VSIGAGHNLNVTGSSVAGDNRVSLAAGNNLNIGTLTESNRETHLKQEKKSGLMSSGGV
                     GFSVGSQSLKVTDTATDTTQKGSTVGSVHGDVSLQAGNRLTVNGSDLIAGRDMALSGK
                     EVSITAATDQHVQTHTVEQKTSGLTLALSGTAGSALNTTVETVQAAKSAGNSRLEALQ
                     GVKAALSGAQAVQAGRLADAQGADAGNNNTVGISLSYGSQSSKSEQQSEQTVAKGSTL
                     TAGNNLSIQATGSGVKGVDGDLTIQGSQIKAGNNVLLQANRDVNLVSAENTSKLEGKN
                     TSSGGSVGVGVGVGSGGWGISVSASANQGKGSEKGNGTTHTETTVDAGKQLTIISGRD
                     TTLTGAQAGGETVKVDAGRHLTLTSEQDSDRYDSKQQNASAGGSFTFGSMSGSASVSL
                     SRDKMHSNYDSVQEQTGIFAGRGGFDVTTGQHTQLNGAVIASTATADKNRLDTGTLGF
                     SDTENRADFKTEHQSAGLSTGGSVAGNFLGNMANNLLVGANHEGHADSTTQSAVSAGN
                     ITIRDTQSQKQDVADLNRDAAHANQTLSPIFDREKEQQRLQQAQLIGEIGNQVADIAR
                     TEGQIAGEKAKRDPAALNQARAELEAAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQ
                     AIQAATAAVQGLAGGNLSAALAGGAAPYLAEVVKTMTTDPVTGEVNKAANVTAHAVVN
                     AALAVAQGNNALAGAAGAATGEVVGMIATQMYGKPVSELSEAEKQTVSTLATVAAGLA
                     GGLVGDSGGAAVAGAQSGKTTVENNYLSSKQIDAWSAEMKSCQAKGGDCGGIIKKYEE
                     LSTAQQKQLISDCAASPATCQQKYGDVLTDSLAVKQAIDRALGEDIPIKMVYDLTATF
                     AQQMQAEGVVATNKVSEALQEEYGLDEVQAGIVASAAASAFGGISKVKGNSQPKIEQI
                     LKPEKNWETARNKALDLVGNLGADSKPVIGRLEVSAGNGKVIGRQSSDGKVGWRVDYD
                     PEKGTHINIWDYSQGKGPGKAVKQVIPFEGNEKSFETILKQLNR"
     gene            complement(7928..8332)
                     /locus_tag="ACHOQW_003227"
                     /pseudo
     CDS             complement(7928..8332)
                     /locus_tag="ACHOQW_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8390..9622)
                     /locus_tag="ACHOQW_003228"
                     /pseudo
     CDS             complement(8390..9622)
                     /locus_tag="ACHOQW_003228"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
     gene            complement(9638..10165)
                     /locus_tag="ACHOQW_003229"
     CDS             complement(9638..10165)
                     /locus_tag="ACHOQW_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9574264.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            complement(10175..11254)
                     /locus_tag="ACHOQW_003230"
     CDS             complement(10175..11254)
                     /locus_tag="ACHOQW_003230"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9574265.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSFDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQAILDAGVSWQRGTRWFGAQPAPEEIFGEATALSKILQLNA
                     SFNLPFTLAGQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQALYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRGQLFSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFRGF"
     gene            complement(11252..11827)
                     /locus_tag="ACHOQW_003231"
                     /pseudo
     CDS             complement(11252..11827)
                     /locus_tag="ACHOQW_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
BASE COUNT         2478 a         3714 c         2890 g         2980 t
ORIGIN      
        1 ccggcgcaac agaataaatc aaccatcgtc agactgcgat cattcgtatg catcgctgat
       61 gatgcgcccg aaaaacgtgt caatatgagt tcgccggaat attaccttac gaaaaaacgt
      121 gtaaagctgt gacagatgac gcgcaaaaaa aatcccggca tttgcgagct ttcccactat
      181 ttcttgattc ctaaacggaa catatcagtt gggaataaag gttgtattat cacttcatca
      241 ttaaaaatga ataatttggg cgataaagct gttacgtcat agatattttc agcgattaat
      301 cttagatttg acctaaaaac tggaatactt gcatcatctg caaagacaaa catgtcatcg
      361 ttttttacag agtttgcaca caatagctca tcaaaactct cataatcaga atgccagatc
      421 tctgcccaag taacacttcc tttcgcgaga gggtacttat ttaaaatgcc cagagcctcc
      481 tgctgagcca gcccctctac tatattaaaa tcagcactca gtgcttcccg acactcatca
      541 aataaagtca tttgctctac ctgtttagct gtttcaatat tgtttcaaaa gacttttcat
      601 tgccttcaaa aggtatgact tgcttcaccg cttttccagg gcctttaccc tgcgagtaat
      661 cccatatatt gatgtgtgtg cctttttcag gatcataatc aacacgccaa ccaacttttc
      721 cgtcgctgga ttgccgccct atgactttcc cattaccagc gctgacttcc aaacgtccga
      781 taactggctt agagtccgct cctaaattac ctacaagatc caatgccttg tttcgagcag
      841 tttcccagtt tttctctggc ttgaggattt gttcaatttt aggctgggaa ttccctttaa
      901 ccttactaat tcccccaaat gctgacgccg cagcgctcgc gactattccc gcctgaactt
      961 catccaaacc gtattcttct tggagtgctt ccgagacttt gttggtcgcg acaacccctt
     1021 cagcctgcat ttgctgcgca aaagtcgccg tcagatcgta gaccattttg atcgggatat
     1081 cttcacccag agcacgatct attgcctgct tcaccgccag actgtcagtc agtacatcac
     1141 cgtatttctg ctggcaggtt gccgggctgg ctgcacagtc actgatcagt tgcttttgct
     1201 gtgccgtact caactcttcg tactttttga taataccgcc gcagtcaccg cctttcgcct
     1261 gacaggactt catttcagcg gaccaagcat ctatctgctt actgctcaga tagttattct
     1321 caacagtcgt cttacctgac tgtgcgccgg ctacagcagc cccccctgag tcgcccacca
     1381 ggccaccggc caagccggca gcaaccgttg ccagtgtcga gaccgtctgc ttctctgcct
     1441 cgctcagttc actcaccggc ttaccgtaca tctgtgtggc tatcattccc accacctcgc
     1501 cggtggctgc acctgcagca ccggcaagag cattatttcc ctgtgccacc gccagtgcgg
     1561 cattcaccac ggcatgggcg gtcacatttg cagccttatt cacctcacct gtaaccgggt
     1621 ctgtggtcat ggttttcact acttcagcca gatacggtgc tgcaccacct gccagggctg
     1681 cactcagatt accgccggcc agcccctgta ctgctgccgt tgcggcctgt atcgcctgct
     1741 ggtatttgcc tcccgttcca aaacctgagg ccgccatacc gttattgtag gcccgctgtg
     1801 ccacatcctg ttccgtgaac ggttttcctg ctgcctcaag ctctgcgcgg gcctgattca
     1861 gtgctgccgg gtcccgcttc gctttctcac cggctatctg tccttccgtt ctggcaatat
     1921 ctgccacctg gttaccaatc tcaccaatga gttgcgcctg ctgcagtcgc tgctgctctt
     1981 tttccctgtc aaagatgggg gacagcgtct gatttgcatg tgcagcgtca cggttcaggt
     2041 ctgccacatc ctgtttctgg ctctgcgtat cccggatggt gatattcccg gcagacactg
     2101 ccgactgcgt ggtactgtcc gcatggcctt catgatttgc accgaccagc agattgtttg
     2161 ccatgttccc caggaaattt ccggcaacac tgccaccggt actgagccct gcactctgat
     2221 gttccgtttt aaagtcagcc cggttttcag tgtcactgaa gcccagtgtg ccggtatcca
     2281 gccggttttt atctgccgtt gccgtggacg caatcacggc accgttcagt tgtgtatgct
     2341 gtccggtggt gacatcaaac ccgcccctgc cggcaaaaat ccccgtctgc tcctgcacac
     2401 tgtcgtagtt actgtgcatt ttatcccggc tgaggctcac actcgccgaa ccgctcatgg
     2461 acccgaaagt gaagctgcct ccggcgctgg cattctgctg tttactgtca taacggtcac
     2521 tgtcctgctc actggtcagt gtcagatggc gaccggcatc cactttcacc gtttcaccgc
     2581 cagcctgcgc gccggtaagc gtggtgtccc gcccgctgat aatggtcagc tgttttcccg
     2641 catccaccgt ggtttctgta tgggtggtac cgttgccttt ttcactgccc ttaccctggt
     2701 ttgcgctggc tgaaacactg ataccccagc ctccggagcc cacaccaaca ccgacaccca
     2761 cgctgccacc actggatgtg ttctttcctt ccagctttga cgtgttttct gctgatacca
     2821 gattcacgtc acggtttgcc tgcagcagaa cgttgttccc ggcctttatc tgactgccct
     2881 gtatcgtcag gtcaccatcc acgcccttca cgccggagcc ggtggcctga atactgaggt
     2941 tattacctgc ggtcagtgta ctgcccttcg ccaccgtctg ctctgactgc tgttctgatt
     3001 tcgaagactg gctgccgtaa gagaggctga tgcccaccgt gttattattt ccggcatcgg
     3061 cgccctgcgc gtctgccagt cgtccagcct gcacggcctg agcccccgag agcgcggcct
     3121 tcaccccctg cagggcttca agccggctgt ttccggcaga tttcgccgcc tgtacggtct
     3181 ccaccgtcgt attcagggcg ctgcccgctg tgccggacag ggcaagcgtc agaccggagg
     3241 ttttctgctc caccgtatgt gtctgtacat gctggtctgt ggcggcggta atgctgactt
     3301 ccttaccact gagtgccatg tccctgccgg caatcaggtc agagccgttg acggtcaacc
     3361 ggttacctgc ctgaagggaa acatcaccgt gaacactccc caccgtgctg cctttctgtg
     3421 tggtgtctgt tgcggtgtcc gtgactttca gactctggct gcccacgctg aatcccacac
     3481 ctccggagct catcagcccg gattttttct cctgtttcag gtgtgtttca cggttgcttt
     3541 ctgtcagcgt tccgatattc aggttatttc cggcagccag ggagacacgg ttatccccgg
     3601 ccacactgct gcccgtgacg ttcagattat gtccggcacc gatactgacc gtatccccgt
     3661 tcagctcact gcctgtcatg gtccggcggt cagtgacatc atgcgtggta gtggtggttt
     3721 tcgataacat cccgctgctg ccggtcactt tatggtattc gtccagcgaa ctggtattct
     3781 gtgcaccggt cagcgtcacg tcatgacccg ccatcagagc caggcctttc tgagctgaca
     3841 gcgatgccgc ctgtgacgtc atatcccgtc cggcggtcag cgtgacatca ccgttaccgg
     3901 caagcgtgct gcccgtactc tgtgtgacgc cctgcgacag acgattattt ttgtcccagg
     3961 taatattgtc ctgcgcggat gtggtcactg tggaaagcgt catatccctg cccgcactga
     4021 gttgcgtcag gctgtcctta ccctgattca ccaccgttgc agccgtcaga ttcatatccc
     4081 gtccggcctg cagcaccagt cttccctggt cattctgtac atacacgccc gccacactgt
     4141 cgatgctggt gcgttcaaaa tggttctgtc cggcgtcact ttgtgccgtg cgggtggtgg
     4201 tggtcagatt aatatcccgc ccggccatcg ccagcaggct gtccgtggcc tgcagcagcc
     4261 cgccggtact gttaatatcc gtccgggccg tcaggctgac atttgctccc tgaatggtgc
     4321 cggcctggtt gtgaatgttt tctgcactga gctgcacggt ctgcttcccg gccagtttac
     4381 cgctgttaaa caggtcacca tccagcttca tcaccatgtc cctgccggca atcagcgcac
     4441 cggcgctgtt cacatcaccg ggttttacac gggcatacac ctgaggtacc tgaaccgtct
     4501 gcgtgctgcc gtccggcagt gtcaccgtgg tgttaaccag ccagacaata tcaccggtca
     4561 gtaacgccat ctgctcagca gtcagcgcca cacctggtgt caggttgtat tgcttaccaa
     4621 atgcaatacc cgcatccatt aacgccttaa actgctcttc atcattactg tatccgtcca
     4681 ggtaccgttg cccggtcaga gcgacaacct gctcacgaat cagacgctgt tcgtaaaaac
     4741 catcccccag gcgtttgagc atgttatccc cgttctgaga aaacgccttc tgcatgtaat
     4801 cactgcccag ccacgtcttc tggttcgtaa aacgcgggtc cgtttcaacc agatatggca
     4861 cattcactgc cggatttgtt ttaaacaggc tgctgtccgg cagacgggta tccggcccgg
     4921 caacatggat actgccctga ggtgcgctga cttcaaactg ctgccccggc aacagaatga
     4981 ccggttcacc ggcagacacc accgggcgaa gcggtatccg gtcagtaccg gctactgtcg
     5041 catccacatt tccggtcagt cctgtctgta catccgttcc ctgtggcttc aggggagaaa
     5101 gcgtgacctg gctgccctga acctgcccgt ggctggtgag ctctcccggc ttcagcgtga
     5161 tggtctgaat ggcagtgggc ggctcgtaag gggaaatgct ttccccctgt ttgtccgtcc
     5221 ctttcttttt gatgcggtag taatatgttg atgtgccttt gtcagtaaca tgttgctctc
     5281 cgctgacact gacgttttcc acgtttcctg acggaatggt cagtttaccg cctgcaacaa
     5341 cctgactctt atcattaaac agtttgtctg ccacaatcgt catgccggca ccggacagaa
     5401 tttttcccgg gtcggattct tttacccggg tttcttccac ggtacgggtg taatcgtact
     5461 gcgtgaattt atctctggcg ccttccggtg tatgcagttt tttcagtgag ttactggaat
     5521 cgttatccac ataaacgccc ggttcaccgg cgctccagcg tttatctgag ccgctgagct
     5581 gatactcagt caccttttcc gtggaaacca ccacgcgttc cgtggtgaaa tggtcattga
     5641 cgttattgat ttgtccggca ctcagtacca gatatccggc ggcttcaata gtggcactgt
     5701 gattattcag tacaccggct ttccccgtag cggcaccatt cgcatccagc atgccaccag
     5761 tatgcatatc acctgcactg taaatcaggc tgtgcgtccg gttattcaga gtctgtaccc
     5821 ctaagtccac acgctcacgc ccggccagca ccgccgccac gccattttca tccagattat
     5881 taaatgtggc agcgctgaca ccaacagcat cgccgtagat tcgcccggta ccggaattcg
     5941 tcagggtgtt cgcctgaaga tgcgtatatt tcccgtcaat cagcccacgg ttcagcagtg
     6001 tgtccgtcac gttaagccag gtctgcccgg cactgatttc acctttctct gtgttttcaa
     6061 ggcggatgct gttcaggtca agccggctgc ctgccagcag gctgccggta ttagagacat
     6121 cgccattaac ggaaagcgta aggtttccgt tcgcaatagt ctgaccactg ttgctgaacg
     6181 ttgtgttact tttcagcgtc atatcgccca gcgaaagcag tttcccgtca ccaccgagct
     6241 gaccggcatc cacagatact gactgcccgg ccttaaccac gccgtcacgg ttcgaaaacg
     6301 ccagctgacg gccactaaca gacaatgtcg cgccggaaga gagcacgcca tcattattac
     6361 tgagcgtcct gtctgccata accgtaagcg ccctgtctgc cagaatactg ccctcctggt
     6421 tatcaaggtc ctgcgtcgtg atgtgtattg tctgcgcttc cagcccctgg ccggtattct
     6481 gcgtgtggcg attgataaac tgcagggcgt tcagcgtaat ggcagaacca ctgcgaatca
     6541 gaccagagac gttgtccaca acgccccgta cactgtcgaa cagaatgtcg cccacagact
     6601 gtatctgtcc gtggctgttc tgcaggcctc ccgtatgcag acggagttca ccttcactga
     6661 ccagccggcc accctcacgg ttatccagcg aggtggtgct caggtcagct gtcgttttcg
     6721 cccccacggt cccgctccgg ttatccattg ctccggtact gacagtcagt tcctgcaggc
     6781 tgttcagcgt accgcctctg ttatccagcg tctggcctgc ggtattaatg ctgatgcctt
     6841 tcccctgaat aagaccggac tgattgttca gcgcctggct gttccagccg agcagaccgt
     6901 ttgccaccac ctgaccggat gtattgttaa ccacgcctgc agtcagggac agagcatctc
     6961 ccgacaaaag cacaccggca gtgctgtcca gtgtgccggc attcagcgtc atcgaaccct
     7021 gactgataac cccgccccgg tcaccactgt tacggttact cagtgtgtca gcacggagat
     7081 tcagcgatgc accgctctgt atcaggccgc tggcatcgtt gatgatatcc gtcgaggaaa
     7141 gggtcagtga ctgctgtgcc accagtctgc cgccctgatt atcagagcgt gtgacatcca
     7201 gcctgctgtt accgttgctg accaccgtcc cctgctgatt ttccagcaca ttcaggtgcg
     7261 cattcaggtc tctgccggcg gcaagcgtcc cttcctgatt actgacgtca ccaccactca
     7321 gcaacatatc cgtcccggcc ttcagtgcgc ctttctggtt catcagcgta cctgacgata
     7381 tctgtaatga cgcctgactc cccagcacgc cctgacggtt atcaagcgtt ccggcgttca
     7441 gggtcatatc cttctgaccc accataaatc cctgctggtt attcagcata ccagcccgca
     7501 ctgtcagggt accggcgctg ctgataccac cccgtgtttt cgtgttccgg ttactaagta
     7561 ccccgtcctg tacatccagc gtcatgtcac tgccggactg aatcagacca tcatcagaga
     7621 ccagttcgct gcctgacaca gacagcgttt tctgtgaaag cagtctgccg gacgtattat
     7681 ccagcgcacc atccagcgtc acggacagcc cgtcagacac caccgtaccc tggtggttat
     7741 taagactccc tgccgaaata tccagcctgc cggcggactc cattcgtccc tccgtattgc
     7801 tgacagactg ctgtgttttt atcctgcccg ttccggcagc agctgaaatc agtccgttgt
     7861 cgttattcag cgcctgtgta ttaatcagga tgtcactgct ggcaagcaga cgtccctgct
     7921 ggttatccac ggcaccggtt gtcttcacct gcagactgcc tgactgcgcc gcctgcactt
     7981 tccccttgcg attatcaagg ctcagtgcat ccagacgcac cgcgccattt gccgcaagca
     8041 gcccttcccg gttatccacg cttcccggca gactcagcga aaaatcccct gtccccgtct
     8101 gaatcagact gcctttacga tttgacaggc tttgtgccga tatctgcagt gtgtcggcat
     8161 ttatcgtacc gccattattg ctgaactgcc cggcagtccg ggcggaaagc gtaccggcac
     8221 tcagttgcgc attctgtgta gacagatttc cactggttgc attcagctca gtatgttgtc
     8281 cctgtatccg gctgttgctt aaatccagcc cctgtccctt cagttgcatg ttcccaccgg
     8341 acagaacctg tccgtgtgcc gccagttcgc cggtggtact gacagtcagg ttgcccgctg
     8401 ccgccagacg tccgtcatct ttcaccccgg cggccagcag actccgctca ccgccgatca
     8461 gacgcgctgt ctgaatccgg gtgttgcgac gtgatgccac agagcctgta tgggtgaata
     8521 caccatcact ctgtatctgc gtatcctgcc ccgcataaac agaaccactg ttatgcagct
     8581 cccgggttgt cgccagatgc acatccgttt tcgcactgat actgccactg ttttcaattc
     8641 gtccgtcagc cgtcagggta acctcccctg cctgtgcacc aatatgaccg gcgttacgaa
     8701 cacccacccc cgtttccgta ccgacaaggc gaatttttcc ggcatacatt ccaccaaggc
     8761 tggacacatc cagcgccacc tgtggctgcg aagaggcatc agcggctttt ttctcagtct
     8821 gcccgtgtgc aatatccaca ttgttacgtc cggtggtcac tttcaggtcc tgcgcccaga
     8881 taccggcatt aatcttcaca gaccgggcaa tgatatcggt ataatccgtg cgcgaggcat
     8941 ccatcccttt gcccgtaatc tgaatctcac cacgttcaac actgaatccg gtcagactgc
     9001 cgtttttcat ctgcggctgc cccgtggtca gcgtggcccg gttcgcatta ataaaaccgc
     9061 acccgtcgca actgatgccg gccgggttcg caatcaccac ctgcgccttt ttcccggcaa
     9121 cctcaacaaa accgttcaac tggctgggat tgcgactgtt cacttcatta agaatgattc
     9181 tggcctctcc cctggccagc cacgggttcc cggccaccat gccacccaac tgagtctgca
     9241 cattgttatg ggcattattc agaatgacgc cctggttacc gacgtcgaac tggctgtatg
     9301 tattatgtga aaccccgccg gcagaaggcg tctgaatatt cacctgcgga atgccgctgg
     9361 ccgtctgcgt aatcgtgggc tgctgatgcc ccggtgcgcc atggtctgcg acaatgcctg
     9421 cagcactgac agaatgcacc atccccgacg ccagccacaa agagaatgca agcggcgtga
     9481 cacggcagat acgttgcctg tgtggatatc ctgtgcggga ggacagtgat gtaccggcgc
     9541 gaccggaacg ggcaatatct gccaccacca tcagcattcc ccgggctttg ttaaaaataa
     9601 tgcggtaaca atttctgttc attctgataa acctgactta tctctccggc acatccacag
     9661 caagcgggtg ctgcacacgc cactggcgcg cctcttcacg actgacctgt gcaccccgga
     9721 aattcatcac ccgcaacccg gtctgctgcc cacggtggta cagcgaacgc cagcacagac
     9781 ggggaaaaat atcctgccgg acaagacggc tcatatccgg agtgtgtcca aaaggggcaa
     9841 cccagtcaca gacccacatc cggtcgccgc tgttccagtc cgactgaggc atgagaattg
     9901 ccggctgcgt aagataacgc gcctctgcct ccgggctgag ccaggcctgg ctcatgaaaa
     9961 aaaccggtcg tccctgctct gttgccacca catactgtcg gtgcttaatc accggcagca
    10021 gcaggtccgg cagggtatgc aatggcgcat cacgatgcag gggggagtgc atccacagcc
    10081 agacggccgc ccccagaact tccgcctcat tgaccggctc accggaatac aggggactca
    10141 gaacgtcata tttcccgtta cgcatgaaat tttctcagaa accccggaac agattaaaac
    10201 cggctgtcac atcactcgtc ataaaacccg ccggttttga aaacggacga ccgatgaaaa
    10261 cgtcataatc cgtactgaac agttgccccc tcagccccac aacaccaccg gccagatgac
    10321 gtcccaccag gtattcactg ccggctcccc ccacctcgcc gtaatccatt cccagatata
    10381 acgcctgctc cggcagtggc gtacgccagg ccaggtcgtt gcggatatac cagccgttac
    10441 tggcattcag cgttcgctcc ccgtcaaaac cacgaaccgt ccagcggtta ccgatggcaa
    10501 actgttcctg tggagtcagc ggggtattgc tcagctggcg ctgataccgg aaggaataac
    10561 tgaagggctg ccctgcaagg gtaaagggca gattaaagga tgcattcaac tgcaggatct
    10621 tactgagcgc cgttgcctca ccgaagattt cctccggtgc cggctgtgca ccaaaccagc
    10681 gggttccccg ttgccagctg acgccggcat ccagtatggc ctgcgaaata taatgacggt
    10741 gctgcagtcc aagtcgccat gccgccgtct gccggcgctg gacctggatt tccgtatcat
    10801 taatgtagtt acgcgacctg cgggcctgaa catcgtaagt cagcaccgtt ttctgcgtcc
    10861 cgttacggtg cagtacccgg ctcagctgca tgcccagatt ctgactctcg ccactgtact
    10921 ggtaatcctc aaccgcacca gctaccgtct gatggtaatc gtaatcactg gcggtgacgg
    10981 aaaactgcca gtagccgaaa ggcactgagt aatgggccgt gtagttttta ctgcccttac
    11041 ccccgtaatg tggaaaatta tgggtggcag aaacgtacag caggtcgctc agggaaaaag
    11101 gattatccag agaaaatgtt ataccaccga gataacgccc tgtgctgcgg gtacctgaat
    11161 cgtcaaacga aaaccccagt cgccagtatt tgtcctgttt gcgggtaata agaatgtcac
    11221 tttcccctgg ctgctcaccg ggtacaattt ccatatcagc ctgcacaccc ggcagtcgtt
    11281 gcatattctc cagcccctgt tcaatgtccc gtaaatccag cagagagcct tcgtgtgccg
    11341 gaaatgagga atacaactga atatagtcat cactgcccgg tgtcagacgc acatgtcgta
    11401 caacaccggg aataataacc agccggagga taccgctttt taaatcctgc gaaggtgcca
    11461 gaacccgggt ggtgacataa ccatgatcaa ccagacggtt ctgcaatgta ctcatcagca
    11521 gattaattcc tttcgccccc aggcaatgcc cgaccgcccc gttggcgatt ttttgtaaag
    11581 gcagccagtg tggtaacgca tcagccccct tcagttccgt ctgtttaatc tgaaaacagg
    11641 gcgtttcaac aggaaaatta atcttatggg caaaagagcc aggtgcagaa agacgaacat
    11701 caggggctga aggcgtcagt tgttgctcaa gggcttcctg acgctgctgc tgaatgataa
    11761 atgtatcctc tccactacgc cggatatccg cggcctgaag agataaaggc gataataaaa
    11821 cagacatcag tgtcagtgac agcaaggacg gacagcgccg gaataagctt tgttcctcag
    11881 ccctctcgtt cctgcaacat aattttccag tcaaacgaaa aaccttttat tcatgttata
    11941 ccaacaatca ccagcctcct gacgatacct cccaccatgt tttgcgatgg cacgctaccg
    12001 cccttccggt atttacccgc atcaaccggt accagaatgc tgaataataa atctgccaat
    12061 tt
//
LOCUS       DAZKFZ010000131        11995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000131 DAZKFZ010000000
VERSION     DAZKFZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..582
                     /gene="yebB"
                     /locus_tag="ACHOQW_003232"
     CDS             <1..582
                     /gene="yebB"
                     /locus_tag="ACHOQW_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP9574266.1"
                     /translation="EYEIGDKVFTCIGDALFGQISAASNCWSNHVGIIIGHNGEDFLV
                     AESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHTGF
                     KYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWER
                     KTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(584..1105)
                     /gene="ruvC"
                     /locus_tag="ACHOQW_003233"
     CDS             complement(584..1105)
                     /gene="ruvC"
                     /locus_tag="ACHOQW_003233"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP9574267.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1140..1880)
                     /gene="yebC"
                     /locus_tag="ACHOQW_003234"
     CDS             complement(1140..1880)
                     /gene="yebC"
                     /locus_tag="ACHOQW_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9574268.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1909..2361)
                     /gene="nudB"
                     /locus_tag="ACHOQW_003235"
     CDS             complement(1909..2361)
                     /gene="nudB"
                     /locus_tag="ACHOQW_003235"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP9574269.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2479..4251)
                     /gene="aspS"
                     /locus_tag="ACHOQW_003236"
     CDS             complement(2479..4251)
                     /gene="aspS"
                     /locus_tag="ACHOQW_003236"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9574270.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            4561..5127
                     /gene="yecD"
                     /locus_tag="ACHOQW_003237"
     CDS             4561..5127
                     /gene="yecD"
                     /locus_tag="ACHOQW_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9574271.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5124..5942
                     /gene="yecE"
                     /locus_tag="ACHOQW_003238"
     CDS             5124..5942
                     /gene="yecE"
                     /locus_tag="ACHOQW_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9574272.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5995..6390
                     /gene="yecN"
                     /locus_tag="ACHOQW_003239"
     CDS             5995..6390
                     /gene="yecN"
                     /locus_tag="ACHOQW_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP9574273.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6431..7174
                     /gene="cmoA"
                     /locus_tag="ACHOQW_003240"
     CDS             6431..7174
                     /gene="cmoA"
                     /locus_tag="ACHOQW_003240"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP9574274.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7171..8142
                     /gene="cmoB"
                     /locus_tag="ACHOQW_003241"
     CDS             7171..8142
                     /gene="cmoB"
                     /locus_tag="ACHOQW_003241"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP9574275.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8307..10736)
                     /gene="torZ"
                     /locus_tag="ACHOQW_003242"
     CDS             complement(8307..10736)
                     /gene="torZ"
                     /locus_tag="ACHOQW_003242"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP9574276.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10761..11861)
                     /gene="torY"
                     /locus_tag="ACHOQW_003243"
     CDS             complement(10761..11861)
                     /gene="torY"
                     /locus_tag="ACHOQW_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP9574277.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2742 a         3182 c         2928 g         3143 t
ORIGIN      
        1 gaatatgaaa ttggagataa agtctttaca tgtataggtg atgccttatt tggtcaaata
       61 tcagctgcat caaattgctg gagtaatcac gtcgggatca ttatcggtca taacggtgaa
      121 gacttcctgg ttgcagaaag ccgggttccc ctttcaacca tcaccacgct atctcgtttt
      181 attaaacgtt cgtctaatca acgctatgct ataaagcgat tagacgctgg gctgacagaa
      241 caacaaaaac aacgaattgt tgaacaggtt ccctcccggc tacgcaaact ttaccacacc
      301 ggttttaaat acgaatcttc gcgccagttc tgttcaaaat ttgtttttga tatttataaa
      361 gaagcgctat gtattccggt gggtgaaata gagacgtttg gagaattgtt aaatagcaat
      421 ccgaatgcaa aactcacttt ctggaaattc tggttcctgg gttctattcc gtgggagcgt
      481 aaaaccgtca cgccagccag tttgtggcat catccaggtt tggtgttgat tcatgcggag
      541 ggagtggaaa cgcctcagcc ggaactgacc gaggcggtat aacttaacgc agtcgccctc
      601 tcgccaggtt cagccgcgat tcgctcatct gcatcgcatt ctgactaacg tggcagtggg
      661 tgatggcaat cgccagcgca tcggcggcat ccgcctgtgg attagcgggc agtttcagca
      721 aggtgcggac catatgctgc acctggcttt tttcggcact accaatacct accaccgttt
      781 gctttacctg acgtgccgcg tattcaaata ctggcaactc ctgattcacc gccgccacaa
      841 tcgccacgcc gcgcgcctgg cccagtttca gggctgagtc agcgttcttt gccataaaga
      901 cttgttcaat ggcgaaataa tcgggctgga actgggtgat gatttccgtc acgcccgcat
      961 agatgagttt cagacgagac ggtaaatcat ccactttggt gcgtatgcat ccgctaccca
     1021 ggtaggacag ttgcctacct acctggcgga tgacgccgta gccggtcacg cgcgaacccg
     1081 gatcaatgcc gagaataata gccatcacgc gtctccgttt tgctgtttag caggcctcat
     1141 cagagagtcg ctgcgacctc atcagagatt tcaccgttat ggtaaacttc ctgcacgtcg
     1201 tcgcaatctt ccagcatatc gatcagacgc atcagtttcg gtgcggtttc tgcatccata
     1261 tcagctttgg tagacgggat catggaaact tccgcgctgt ctgctttcag acctgccgct
     1321 tccagagcgt cgcgcacttt acccatttct tcccatgcag tgtagacatc aatcgcgccg
     1381 tcatcatagg tcacaacgtc ttcagcacct gcttccagtg ctgcttccat gatggtgtct
     1441 tcatcgcctt tctcgaagga gatgacgcct tttttgctga acagataggc tacggaacca
     1501 tcagtaccga ggttaccgcc acatttgctg aatgcatgac gcacttcagc aacggtacgg
     1561 ttgcggttgt cagacagaca ttcaatcatg attgccgtgc cgccaggacc gtaaccttcg
     1621 tagatgatgg tttccatgtt cgcatcatca tcaccgccca caccacgtgc aattgcgcgg
     1681 ttcagagtgt cacgggtcat gttgttagac agtgctttat caattgctgc acgcagacgc
     1741 gggttagcgt ccggatcgcc accgcccagc ttagccgcgg ttaccagctc acgaatgatt
     1801 ttagtgaaga ttttaccgcg cttagcatcc tgcgcagctt tacgatgtct ggtgttggcc
     1861 catttactat gacctgccat aaaaatatct ccagatagcc ctgcctgttc aggcagcgtt
     1921 aattacaaac tgttcaatcg cctgccggtt gctccaggac ttagtgagcg ccgccgcagc
     1981 aggcgcatca agccacttgt aagccagatg ttcagtgaaa acgatctgcc gctcatgcgg
     2041 aagcgcaaga cagaaccatg attccgtatt acgcgtcacg cccggcgcat agcgatgacg
     2101 taaatgtgaa aaaatttcaa actctaccgt gcgctgacag tcaattaagg tcagttgttc
     2161 agcgacaaca tcaatggtga cctcttcctt tacttcgcgc atggcagctt gcggcgcggt
     2221 ttcaccctct tccacgctgc cggttaccga ctgccagaaa tcggggtcat cacgccgctg
     2281 caacatcagc acccgtttcg tatcttgtgc gtagatgacc actaagatcg aaacgggacg
     2341 cttatacact ttatccttca cgctgcctct tcgttgactg ccttcgctca tcccattcac
     2401 atagttatct atgctcatgg gagttcactc agttgccgcc tcgatgcaac gcgaatgatt
     2461 tcgtgtattt gagtcatatc agttattctc agccttcttc acaacctgaa tgctcagctc
     2521 agccagtgca gccgggttag caaagctcgg tgcttcagtc atcagacacg ctgccgccgt
     2581 ggttttcggg aaggcgataa cgtcacggat attgtcggtg ccggtcagca gcatggtcag
     2641 acggtcaaga ccgaatgcca gacctgcgtg cggcggagta ccgtatttca gagcgtcgag
     2701 caggaagccg aatttctcgc gctgttcctc ttcgttgata cccagaatac caaacaccgt
     2761 ctgctgcata tcaccattat ggatacgtac tgaaccaccg cccacttcgt aaccattgat
     2821 gaccatatcg taagcgttcg ccaccgcatt ttccggtgca gctttcagct ctgcagccgt
     2881 catgtctttc ggtgaggtga acggatggtg cattgccgtc aggccgcctt caccgtcgtc
     2941 ttcaaacatc gggaagtcga taacccacag cggtgcccat ttgctttcgt cggtcagacc
     3001 aaggtcttta cccactttca ggcgcagtgc gcccatcgcg tcggcaacaa ttttcttgtt
     3061 gtcggcaccg aagaaaatca tatcgccatc ttgcgcggca gtacgatcca ggatggcttc
     3121 gatgatttct gcattaagga acttcgctac cgggctgttg ataccttcca gacctttcgc
     3181 gcgttcgtta actttgatgt aagccagacc tttcgcgccg tagattttaa cgaagttacc
     3241 gtattcgtcg atctgcttac gggtcagcga tgcgccaccc ggaacacgca gagcggcaac
     3301 gcgacctttc ggatcgttcg ccggacctgc aaatactgca aactcaacag atctcagcag
     3361 atcagcaacg tcggtcagtt ccatcgggtt acgcagatcc ggtttatcag aaccataacg
     3421 gcgttcggct tccgcaaagg tcattactgg gaaatcgccc agatccacgc ccttcacttc
     3481 cagccacaga tggcgcacca gcgcttccat cacttcacgc acttgcggcg cggtcatgaa
     3541 agaagtttcc acatcgatct gagtaaattc aggctgacga tcagcgcgca ggtcttcgtc
     3601 acggaagcat ttaacgatct gatagtagcg gtcaaagccg gacatcatca gcagctgttt
     3661 gaacaactgt ggggattgcg gcagtgcgta gaatttacct ttgtgcacac gagaaggcac
     3721 caggtagtca cgcgcgcctt ccggcgtggc tttggtcagc atcggagttt cgatgtcgag
     3781 gaaaccgtgg tcatccataa aacggcgcac caggctggtg attttagcgc gggttttcag
     3841 gcgctgagcc atttccggac gacgcaggtc gaggtagcgg tatttcagac gcgcttcttc
     3901 ggtgttgacg tggttagagt caagcggcag aacatctgcg cggttgatga tagtcagcga
     3961 ggacgccagc acttcgattt cgccggtcgc catatcgcgg ttaatatttt tttcgtcacg
     4021 cgcacgtacg gtgcccgtga cctgaatgca gaactcatta cgcagttcag aggccagctt
     4081 taacgcgtcc gcacgatccg gatcgaaaaa tacctgcacg ataccttcgc ggtcgcgcat
     4141 atcgatgaag atcaggctac caagatcacg acgacggttg acccaaccac acagagtcac
     4201 ctgctgcccc acgtgggaca aacggagctg tccacaatat tctgtacgca tgagatatcc
     4261 cttaacttag ctgccggcgg atgccccctg ctgcgcaggt gaccaagtcg cagcgttagc
     4321 tgtatgtcac aactgaatga aaaaaggcgg ctattatact ggaaattctg ccgcaccgta
     4381 agagcctggc ccgcgctgga acgcctcgtt accactttat atcgggcctg aaatcagact
     4441 ctacgccagt ttgctataaa ggtgttgccc gaactcatac aaattaacaa aatttgtcgt
     4501 tccgccatcg gctaatcgca ttaaggtgtg aggcacgatt ttgttttttc aggagtcatc
     4561 atgcttgaac ttaatgctaa aaccactgca ctggtggtga tcgatttaca agaaggcatc
     4621 ttaccttttg ccggtggtcc acatactgcc gatgaggtag ttaatcgcgc cgggaagctg
     4681 gcggcgaaat ttcgcgccag cggtcagccc gtgtttctgg tgcgcgttgg ctggtctgcc
     4741 gattacgccg aagcattaaa acagccggtt gatgcccctt cccccgctaa agtgttgccc
     4801 gaaaactggt ggcaacatcc agctgcatta ggtgcaactg acagtgatat cgaaataatc
     4861 aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt tgcaattacg ccgccgaggt
     4921 atcgatacaa tagtgttatg tgggatctcg accaatatcg gagttgaatc caccgcccgc
     4981 aatgcctggg aacttggctt taatctggtg attgccgaag atgcctgtag tgccgctagc
     5041 gccgagcagc acaataacag tatcaaccat atctacccgc gcatcgcccg tgtgcgtagc
     5101 gtggaagaga tcctcaacgc gttatgattt acatcggtct accgcaatgg tcgcatccta
     5161 aatgggtgcg gttggggatc accagccttg aagagtatgc ccgtcacttt aactgcgtgg
     5221 agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac
     5281 agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag
     5341 cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
     5401 cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
     5461 cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc
     5521 atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc
     5581 gcggcgttaa tcaggtgatt ttagacagcc gcccggttca tgcagcacgt ccacacagtg
     5641 aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg
     5701 cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
     5761 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
     5821 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
     5881 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
     5941 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
     6001 agcgcgctgt atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
     6061 cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc
     6121 gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg
     6181 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
     6241 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgttccgctg gcgacgttcc
     6301 ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
     6361 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
     6421 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
     6481 ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc
     6541 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
     6601 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
     6661 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
     6721 ctgccgtcgt catattgacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga
     6781 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
     6841 cctggaacct tccgagcgcc aggcgttact ggataaaatt tatcaagggc tgaaccccgg
     6901 cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt
     6961 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
     7021 gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
     7081 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
     7141 actggtggca ttaaaagcag aggacgctgc atgatcgact ttggtaactt ttattctctg
     7201 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
     7261 cagcgcgagc agcagcacgg gttgtttaag cagtggtcaa atgcggtgga gtttctacct
     7321 gaaattaaac cgtatcgtct tgatttattg catagcgtta cggcagaaag cgaagagcca
     7381 ctgagcgcag ggcaaattaa acgcattgaa acgctgatgc gcaacctgat gccgtggcgc
     7441 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa
     7501 tgggatcgtg ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc
     7561 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
     7621 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaat
     7681 gatcagcgcg cgcatttgtt accgttaggt attgaacaac ttccggcact gaaagccttt
     7741 gataccgtct tttcgatggg cgtgctttat catcgtcgtt caccgctgga gcatctctgg
     7801 cagttgaaag atcaactggt caatgaaggt gaactggtgc ttgaaacgct ggttattgat
     7861 ggcgatgaga acacggtgct ggtgccgggc gatcgttacg cgcaaatgcg taatgtctat
     7921 ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc
     7981 cgcattgcgg atgtgagcgt taccactaca gaagagcagc gacgcactga atggatggtt
     8041 accgagtctc tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat
     8101 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc
     8161 ggatgcggcg tgaacgcctt atccagccta caaaaccttg ctaattcaat atattgcagg
     8221 gactatgtag gcctgataag catagcgcat caggcagctt tatgtttgca taacctcagc
     8281 gcccgtttcc gggcgctttt cacgtcttat gcctgaacag ctggctgatc aaacgccgtt
     8341 aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag
     8401 gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
     8461 cacaagccat tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
     8521 atcccgtcag tcaccacagc gcctgtcaaa atctgaccac gtttgttcca gacacggacc
     8581 agatcgccat tcgcaatacc aaagcgagca gcatcttcgg tgtgaatagt gattggttca
     8641 cgatcggcaa ccgcatattt tttacgcagt tgcgcatagt taagctgact gtgtaaacgg
     8701 tgtgccggat gtgcggtcag aagctgtaac tgcttctcgt cggcggtacc tttccactca
     8761 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     8821 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
     8881 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     8941 ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcacct
     9001 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
     9061 atctcttttc cgcctggttt cagtaattcc gcaagttcgg caaacacgtc aaaatcgttt
     9121 cgcgcttcaa attgcggagc gacagcctgc ttcatcggta caatatgctg gttgctgtaa
     9181 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     9241 gcatgtttag cagctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     9301 catgctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
     9361 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
     9421 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgtcattcc gccatcatca
     9481 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
     9541 cgcgtcgggt tgccaccgtt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
     9601 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     9661 tgctggcgct gaattcccca gcctgccatc agcattgtgc gattcgccgc catcagttca
     9721 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgtcca ggccgcgctc
     9781 ttcggagtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtggtg
     9841 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     9901 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atagccaaag
     9961 aactcgatgg tttcagagcg gataggatca atggcaatca ctggtttgcc agattttttc
    10021 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
    10081 ttcagcgggt tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag
    10141 gaggtctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct
    10201 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
    10261 tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca
    10321 aaaatggccg atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt
    10381 aatgcctgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc
    10441 agtggattgt cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
    10501 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
    10561 gaagaaacaa tcttgccgtc cttcacttct acgttcatcg ctccccaacg tcccgcggtg
    10621 aggattttac cgcccttctc ttctgcccat gcgggtagcg gtgctgccga tgtcaccacc
    10681 agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgtcataact
    10741 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa
    10801 taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg
    10861 gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg caataggggc atgacaacca
    10921 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcagtttacg gtccgctaat
    10981 gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca
    11041 gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc
    11101 gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
    11161 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
    11221 ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
    11281 gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
    11341 tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt
    11401 gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
    11461 gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg
    11521 gccatttcct ggcgatgagc ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
    11581 tcatgataaa tatctttaga tgcttttaat ttagcgaata aataatccat ccctgacttt
    11641 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
    11701 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
    11761 gacgttttat gtaaggcttt ttgcgccatc aagaacccgc caccaccaac cacaaccgct
    11821 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taattaaatt
    11881 ccagaacacc gaaatcattg tataaataaa tacacaacga attgactact tgcttaattc
    11941 ggcacaacac tgcctgaaat aatcgctaaa gaatatgatt tattacaatg taatc
//
LOCUS       DAZKFZ010000132        11967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000132 DAZKFZ010000000
VERSION     DAZKFZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..2085
                     /locus_tag="ACHOQW_003244"
                     /pseudo
     CDS             1..2085
                     /locus_tag="ACHOQW_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            2422..3399
                     /gene="glaH"
                     /locus_tag="ACHOQW_003245"
     CDS             2422..3399
                     /gene="glaH"
                     /locus_tag="ACHOQW_003245"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP9574278.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3419..4687
                     /gene="lhgO"
                     /locus_tag="ACHOQW_003246"
     CDS             3419..4687
                     /gene="lhgO"
                     /locus_tag="ACHOQW_003246"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP9574279.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4710..6158
                     /gene="gabD"
                     /locus_tag="ACHOQW_003247"
     CDS             4710..6158
                     /gene="gabD"
                     /locus_tag="ACHOQW_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9574280.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6172..7452
                     /gene="gabT"
                     /locus_tag="ACHOQW_003248"
     CDS             6172..7452
                     /gene="gabT"
                     /locus_tag="ACHOQW_003248"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP9574281.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7690..9090
                     /gene="gabP"
                     /locus_tag="ACHOQW_003249"
     CDS             7690..9090
                     /gene="gabP"
                     /locus_tag="ACHOQW_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP9574282.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLWMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9111..9773
                     /gene="csiR"
                     /locus_tag="ACHOQW_003250"
     CDS             9111..9773
                     /gene="csiR"
                     /locus_tag="ACHOQW_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP9574283.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9774..10223)
                     /gene="kbp"
                     /locus_tag="ACHOQW_003251"
     CDS             complement(9774..10223)
                     /gene="kbp"
                     /locus_tag="ACHOQW_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP9574284.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10307..10465)
                     /gene="yqaE"
                     /locus_tag="ACHOQW_003252"
     CDS             complement(10307..10465)
                     /gene="yqaE"
                     /locus_tag="ACHOQW_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9574285.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10648..10947
                     /gene="ygaV"
                     /locus_tag="ACHOQW_003253"
     CDS             10648..10947
                     /gene="ygaV"
                     /locus_tag="ACHOQW_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9574286.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10957..11475
                     /gene="ygaP"
                     /locus_tag="ACHOQW_003254"
     CDS             10957..11475
                     /gene="ygaP"
                     /locus_tag="ACHOQW_003254"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP9574287.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(11528..11932)
                     /gene="stpA"
                     /locus_tag="ACHOQW_003255"
     CDS             complement(11528..11932)
                     /gene="stpA"
                     /locus_tag="ACHOQW_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP9574288.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2980 a         2947 c         3104 g         2936 t
ORIGIN      
        1 gacagagaaa ttatatcaac ttttcacgca gaaaaagtca ttaatgatct gttaaaaaac
       61 gattataaaa tttccctttc tccagataca acttaccgag agctgaataa agctgcacag
      121 cgtagcatta cagcgccaga caggatagga gaagggaaaa tatgggttta tcaacgagat
      181 acaatggttg aaagaggtga taacagcggt gttcatcagt atggtccagc tgaacatttc
      241 actcacatta tatctgacaa accgtcccca aaagataagt atgttgcata tgctattaac
      301 attcctgact atgagctagc agccgatgta tataatatta acgtgacgtc accttccgga
      361 cagcaagaaa catttaaaat gttaatcaat ccagaacatc tacggcaaac acttgagcgt
      421 aaatccctta ctgctgttca gaaatcacaa tgtgaaatca tcacccccaa aaaacctggc
      481 gaagcgattc ttcatgcttt taatgccacc taccagcaaa tcagggaaaa tatgcctgag
      541 tttgcacgtt gccattatgg gtatatacaa atccctccag tgacaacttt ccgcgccgac
      601 ggaccagaaa ctcccgaaga agaaaaaggt tactggtttc acgcttatca acccgaagat
      661 ctttgtacca tccataatcc aatgggagat ttgcaggatt ttatcgcatt ggttaaagat
      721 gctaaaaaat ttggtatcga tatcattcct gattatacct ttaactttat gggaatcggg
      781 ggtagtggta aaaatgacct ggattatccc tctgctgata tacgagcgaa gatcagtaaa
      841 gatatagaag gtggtatccc tggctattgg caaggtcagg ttttgattcc atttactata
      901 gatccagtaa caaaagaacg taaacaaatc catccagaag atatacatct cacagcaaaa
      961 gacttcgaag caagtaaaga taatatttct aaggatgaat gggaaaacct ccatgcatta
     1021 aaagaaaagc gtttaaatgg aatgcctaaa acccccccca aaagtgacta ggttattatg
     1081 ttgcaaaatc aatacgttcg tgaaatgcga aaatatggcg tacgaggttt acgttacgat
     1141 gcagcaaaac actcaaaaca tgaacaaata gaaagatcaa taaccccacc gcttaaaaat
     1201 tataatgagc gattacacaa tactaactta tttaacccaa aatatcatga aaaagccgtt
     1261 atgaattaca tggaatatct ggtaacttgc cagttggatg aagaacaaat gtcatcgctg
     1321 ctttatgaaa gagatgattt aagcgccatt gatttttcat tgctcatgaa gacgataaaa
     1381 gctttttcat ttggtggcga tctccaaacc cttgcatcaa aaccgagttc cacaatctca
     1441 agcatcccat caaaaagacg gatattgatt aacattaacc acgattttcc taacaatggc
     1501 aatcttttca atgactttct atttaaccat caacaagatg aacaattagc aatggcatat
     1561 atggccgctc tcccgttcag caggccttta gtttactggg atggccaagt attaaaatca
     1621 acgactgaaa ttaaaaatta tgatgggtcg acgcgtgtcg gcggtgaggc gtggcttaat
     1681 aaaggttgct ctacctatca gcagctctac aatgaattcc acgcattata tatagataaa
     1741 gcaggaatat ggagcgcatt tgagggtgta tttgcaacta aaaacgttct ggcctttagt
     1801 cgtggggatt cggtgaacat taatcactct cctcatgatg gactagttat aataaataaa
     1861 ggaaacgaag aagttgaagg tgcctggcct aacaaattgc aacctggaaa atataaaaac
     1921 atggggagta atagcgttaa cattattatt aataataccc gaaaaattat cccccctggt
     1981 aaagcattta tgcttagagg cggaactcta aatatcaata ttcctgggcg tagcgctctt
     2041 cttttaggga aaacaggaga accgctgaac tatctctatt tgtaatttaa tgttatatct
     2101 gccccgctaa aacggggcag ataatatatt tagtttacta gcggtcattt tgcagtgaag
     2161 ccatttaccg ttttttatcg accagataat ctgttctctg atggtaatcc ccccctaacc
     2221 tgttgcttta gttattcatt tcctgtctca ctttgcctta ataccctacg ttaaatgtta
     2281 ccaatttgtt gctttttatc acaataagaa aacaatatgg cgcatttgtg cgcatttttc
     2341 agaaatgtag atatttttag attatggcta cgaaatgagc atcgccatgt caccctacat
     2401 ctcataagag gatcgcttct gatgaatgca ctgaccgccg tacaaaataa cgctgtcgat
     2461 tcagaccagg actatagcgg attcactctc atcccgtcgg cgcaatcccc gcgtctgctg
     2521 gaactcacct tcaccgaaca gacgaccaaa cagtttctcg aacaggttgc cgaatggccc
     2581 gtgcaggcgc tggagtacaa atcgtttctg cgttttcggg taggtaaaat tcttgacgat
     2641 ctgtgtgcga accagctgca accattgctg ttgaagaccc tgctaaaccg tgctgaaggt
     2701 gcgctgttga tcaatgcggt gggtgtcgat gatgtgaagc aagcggatga gatggtgaag
     2761 ctggcgacgg cggtggcgca tctgattggc cgttccaatt tcgatgcgat gagcggtcag
     2821 tattacgcgc gttttgtagt gaaaaatgtc gataactcag acagctatct gcgtcagccg
     2881 caccgcgtaa tggagctgca caacgacggc acctacgtcg aagagatcac tgactatgtg
     2941 ctgatgatga aaatcgacga gcagaacatg cagggtggaa attcgttgct gctgcatctc
     3001 gatgactggg aacatctgga ccactatttc cgccatccaa tggcgcgtcg cccgatgcgc
     3061 tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt tccatccagt gttcgacgtt
     3121 gatcaacagg gtcgcccggt gatgcgctat atcgaccagt tcgtccagcc aaaagacttc
     3181 gaagaaggcg tgtggttgag cgagctttcg gacgccattg aaatcagtaa aggcattctt
     3241 tctgtacccg ttcccgttgg caaattcctg ttgattaaca acctgttctg gctgcacggt
     3301 cgcgaccgct ttactccgca cccggatctg cgccgtgaac tgatgcgtca gcgtggctat
     3361 ttcgcttacg ccactcacca ctatcagacg catcagtaag cgcaaaggaa ttgagcggat
     3421 gtatgatttt gtgattattg gcggcggcat catcggcatg tcgaccgcca tgcaactgat
     3481 tgatgtctat ccggatgccc gcattgcgtt gctggaaaaa gagtccggcc cggcctgtca
     3541 tcagacgggc cacaacagcg gcgtgatcca tgccggggtt tattacacgc ccggtagcct
     3601 gaaggcgcag ttttgcctgg cgggaaaccg cgccactaaa gccttttgcg atcaaaacgg
     3661 cattcgctat gacaactgcg gcaagatgct ggtcgccacc tctgaactcg aaatggaacg
     3721 gatgcgcgcg ttgtgggaac gcacggcggc aaacggtatc gagcgcgagt ggttaaacgc
     3781 cgatgaactg cgcgagcgcg aaccgaatat caccgggctt ggcggtattt ttgtgccgtc
     3841 cagcggcatt gtcagctacc gcgaagtcac ggcggcgatg gcaaaaattt tccaggccag
     3901 aggcggcgag attatctata acgccgaagt cagcggcctt agtgagcata aaagcggcgt
     3961 ggtgatacgt acccgtcagg gcagcgacta tgaagcatcg acgctgatta gctgttccgg
     4021 gctgatggct gaccggctgg tgaaaatgct cggcctcgaa ccgggcttta ttatctgccc
     4081 gttccgcggc gagtatttcc gcctggcgcc ggagcataac cagattgtta accatctgat
     4141 ttaccccatt cccgacccgg caatgccgtt tttgggcgtg catctcaccc gcatgatcga
     4201 cggcagcgtc accgtcgggc caaacgcggt gctggctttc aaacgcgaag gctatcgcaa
     4261 gcgcgacttc tcatttagcg acacgctgga gattttgggc tcgtcgggga ttcgccgggt
     4321 gctgcaaaac catctacgct caggactggg cgagatgaaa aactcgctgt gcaaaagcgg
     4381 ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg ttaagcgatc tccagccctg
     4441 gcccgccggt gtgcgggcgc aggcggtatc gccggacggc aagctgattg acgattttct
     4501 gtttgtcacc accccgcgca cgatccacac ctgcaatgcg ccctccccgg cagcgacatc
     4561 agcaattcct attggtgcgc atattgtcag caaggtacaa acgctgttgg caagccagag
     4621 taaccccgga cgcacgctgc gagcggcacg tagtgtggat gccttacacg ccgcgtttaa
     4681 tcaataacct ttgaaaacag gatgtagcga tgaaactgaa cgacagtaac ttattccgcc
     4741 agcaggcgtt gattaacggg gaatggctgg acgccaacaa tggtgaagtc atcgacgtca
     4801 ccaatccggc gaacggcgac aagctgggta gcgtgccgaa aatgggcgct gatgaaactc
     4861 gcgccgctat cgacgccgcc aaccgcgccc tgcccgtctg gcgtgcgctc accgccaaag
     4921 aacgcgccaa cattctgcgc aactggttca atttgatgat ggagcatcag gacgatttag
     4981 cgcgcctgat gaccctcgaa cagggtaaac cgctggctga agcgaaaggc gaaatcagct
     5041 acgccgcctc ttttattgag tggtttgccg aagaaggcaa acgcatttat ggcgacacca
     5101 ttcccggtca tcaggccgat aagcgcctga ttgtgatcaa gcagccgatt ggcgttacag
     5161 cggccatcac gccgtggaac tttccggcgg caatgattac ccgcaaagcc ggtccggcgc
     5221 tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca gacgccgttc tctgcgctgg
     5281 cgctggcgga gctggcgatc cgcgcgggca ttccggctgg cgtgtttaac gtggtcaccg
     5341 gttcggcggg cgcggtgggt aatgaactga ccagcaaccc gctggtacgc aaactgtcgt
     5401 ttaccggctc gaccgaaatt ggccgccagt taatggaaca gtgcgcgaaa gacatcaaaa
     5461 aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt ctttgacgat gccgatctcg
     5521 ataaagccgt ggaaggcgca ctggcctcga aattccgcaa cgccgggcaa acctgcgtct
     5581 gcgccaaccg tttatacgtg caggacggcg tgtatgaccg ctttgccgaa aaattgcagc
     5641 aggcggtgag caaactgcat atcggcgacg ggctggataa aggcgtcacc atcgggccgc
     5701 tgatcgatga aaaagcggta gcaaaagtgg aagagcatat tgccgatgcg ctggaaaaag
     5761 gcgcgcgcgt agtttgcggc ggtaaagcac atgaacgcgg cggcaacttc ttccagccca
     5821 ccattctggt ggacgtgccg gccaacgcca aagtgtcgaa agaagagacc ttcggccccc
     5881 tcgccccgct gttccgtttt aaagatgaag ccgatgtgat cgcgcaagcc aatgacaccg
     5941 agtttggcct tgccgcctat ttctatgccc gtgatttaag tcgcgtcttc cgcgtgggcg
     6001 aggcgctgga gtacggcatc gtcggcatca ataccgggat tatttccaat gaagtggccc
     6061 cgttcggcgg cattaaagcc tcgggtctgg gtcgtgaagg ttcgaagtat ggcatcgaag
     6121 attacttaga aatcaaatat atgtgcatcg gtctttaact ggagaacgcg aatgagcagc
     6181 aataaagagt taatgcagcg ccgcagtcag gcaattcctc gtggcgttgg gcaaattcac
     6241 cccattttcg ctgatcgcgc ggaaaactgc cgggtgtggg acgttgaagg ccgtgagtat
     6301 cttgatttcg cgggcggcat tgcggtgctc aataccgggc acctgcatcc gaaagtggtt
     6361 gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct gcttccaggt gctggcttac
     6421 gagccgtatc tggagctgtg cgagattatg aatcagaagg tgccgggcga ttttgccaag
     6481 aaaacgctgc tggttacgac cggttccgaa gcggtggaaa acgcggtgaa aatcgcccgc
     6541 gctgccacca aacgtagcgg caccatcgct tttagcggcg cgtatcacgg gcgcacgcat
     6601 tacacgctgg cgctgaccgg caaggtgaat ccgtactctg cgggcatggg cctgatgcca
     6661 gggcacgttt atcgcgcgct ttatccttgc ccactgcacg gcatcagtga agatgatgct
     6721 atcgccagta tccaccgaat ttttaaaaat gatgctgcgc cggaagatat cgccgccatc
     6781 gtgattgagc cggttcaggg cgaaggcggt ttctacgccg cgacgcctgc gtttatgcag
     6841 cgtttacgcg cgctgtgtga cgagcacggg atcatgctga ttgccgatga agtgcagagc
     6901 ggcgcggggc gtaccggcac gctgtttgcg atggagcaaa tgggcgtggc accagatctc
     6961 accacctttg cgaaatcgat cgcaggcggc ttcccactgg cgggcgtcac cgggcgcgcc
     7021 gaagtgatgg atgccgtcgc tccaggcggg ctgggtggca cctatgccgg taatccgatt
     7081 gcctgcgtgg cggcgctgga agtgttgaag gtgttcgagc aggaaaatct gctgcagaaa
     7141 gccaacgatc tggggcagaa gttgaaagat ggattgttgg cgatcgccga aaaacaccct
     7201 gagatcggcg acgtacgcgg gctgggggcg atgatcgcca tcgagctgtt tgaagacggc
     7261 gatcacaaca agccggacgc caaactcacc gccgagatcg tggctcgcgc ccgcgataaa
     7321 ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct tgtaccgctc
     7381 accattgaag acgctcagat ccgtcagggt ctggagatca tcagccagtg ttttgctgag
     7441 gcaaagcagt agcgccgctc ctatgccgga ggcgacgctg cgcgtcttgt ccggcctacg
     7501 gggatccagg tcggataagg cgtttacgcc gcatccgaca atctgtacgt gaacaggaat
     7561 aaatctatgt tggccgggta aggcggagcc gctctccggc aaaaagaatc aataacaatt
     7621 atacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga
     7681 ggattcagga tggggcaatc atcgcaacca catgagttag gcggcgggct gaagtcacgc
     7741 catgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc
     7801 gtcgccatcg ccgaagcggg cccggcggta ttactggcct atctgttcgc cgggctactg
     7861 gtggttatga ttatgcggat gttggcggaa atggcggttg ccacgcccga taccggttcg
     7921 ttttccacct atgccgataa agctattggt cgttgggcgg gttataccat cggctggttg
     7981 tactggtggt tttgggtact ggttatcccg ctggaagcca acatcgccgc catgatcctg
     8041 cactcatggg ttccaggcat tcccatctgg ttattttccc tcgtcattac ccttgcctta
     8101 actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg
     8161 tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt
     8221 tacccgtatg ccgacgtgag cgggatctca agattgtggg atagcggcgg ctttatgccc
     8281 aacggcttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca
     8341 gaaattgtca ccattgccgc cgcggaatcc gacacgccgg aaaaacatat tgtccgcgct
     8401 accaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtagtg
     8461 gcgttaatac cgtggaatat gccggggctg aaagccgttg gatcttatcg ctcggttctg
     8521 gaattgctca atatacccca tgcgaaatta atcatggact gcgtgatatt actttccgta
     8581 accagttgcc tgaactcggc gctgtatacc gcgtcaagga tgctctactc cttaagccgt
     8641 cgcggtgatg cgcccgcggt aatgggcaaa atcaaccgca gtaaaacccc gtatgtggcg
     8701 gtgttactct ccaccggagc ggcattttta acggtcgtgg tgaactatta cgcacctgcg
     8761 aaagtgttta aattcctgat cgacagctcc ggtgctatcg ccctgctggt ttatttagtc
     8821 atcgccgttt cacagttgtg gatgcgtaaa attctgcgag cagaaggaag cgaaattcgc
     8881 ttgcgcatgt ggctttaccc gtggctcacc tggctggtaa tcggctttat tacctttgtg
     8941 ttggtggtga tgttattccg cccggcgcaa cagttagaag tgatctctac cggcttatta
     9001 gcgataggga ttatctgtac cgtgccgatt atggcgcgct ggaaaaagct ggtattgtgg
     9061 caaaaaacac ccgttcataa tacgcgctga taatgatcag gagtcacacc atgaccatta
     9121 cgtctctgga tggctatcgc tggctgaaga acgatattat tcgcggtaat tttcaaccgg
     9181 atgaaaaatt acgaatgagt ttgctgacat cgcgttatgc acttggcgtt gggccgttac
     9241 gggaagctct ttcgcaactg gtggcggaac ggctggtcac ggtggtgaat caaaaagggt
     9301 atcgggtggc gtctatgtca gagcaggagc tgctcgatat tttcgacgcc cgcgccaata
     9361 tggaagcgat gttagtgagt ctggcgattg cccgcggtgg cgatgagtgg gaggcagacg
     9421 ttctcgcaaa agcgcatctg ctgagtaagc ttgaggcctg tgacgccagc gagaaaatgc
     9481 ttgatgagtg ggatctgcgt catcaggcgt ttcatactgc aattgtggcg ggctgtggtt
     9541 ctcactattt gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgt tatcgattta
     9601 tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga ggataaacac gatcagcacc
     9661 agaccctgac tgcggcggta ctggcgcgag ataccgcgcg cgccagtgag ttaatgcgcc
     9721 agcatttact gacgccaatt cccattatcc agcaggcgat ggctggcaat taactactct
     9781 tccggaatac gcaacacttg ccccggataa attttatccg ggctttttag catcggttta
     9841 ttcgcttcga agattttatt gtacagatta gcgttaccgt agacctgttt ggaaatggca
     9901 ctcagagtgt cgccagactt aacggtataa aactggctgg cagtggctgg tgtcgccgtt
     9961 ttcacctgat catcaacact ggcaataccg gaaatattcc ccaccgcaac aaggattttc
    10021 tccttcgcct cctgactcag gccgtcacca gtgaccgtcg ctttgccatc ggcaatttga
    10081 atgttcactt tatcggcatc aggtataccg gttttgctca gatgctcctg caccttcttc
    10141 gcctgatcat ctttatcgtg ctgacctgta accgcgtccc agagtttttc tccggcatct
    10201 ttcacaaaat tgaacagacc catagctacc tcattcgtta acggaaacac tcagaaagtg
    10261 tagtagagga acgcggcgcg ttaacgccgc ggggatgagc acaggtttaa tcgcgggttt
    10321 gcacccagaa cgcgtgaatc aaaccgggga tatacccaag cagtgtcaac agaatattaa
    10381 taatgaacgc ccaaccgaac cctttaccga gcagcacgcc gagcggcggc agaatgatgg
    10441 tgatgacgat tctccagaaa cccatatgta ctccctataa gaaaattact tattgtttaa
    10501 aaagagattt tatctcttaa gcgtagtaat tttagcggag gctgccagtt tcagcttacg
    10561 cttttactct catttacgct gctgaggctg gtatatgttg catttttagt ttagagttta
    10621 cttaatttag aaaatactta aataactatg accgaactca cgcaattaca ggccagtgct
    10681 gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct gattctgtgc
    10741 atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac cggactgagc
    10801 gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat caacagccaa
    10861 cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc cattatcgcc
    10921 accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac catttcgccg
    10981 catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg tgacgctgat
    11041 gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt gctggaacag
    11101 tcaggtctcc cggctaaatt acgtcgcgat caaattatat tccactgcca ggcaggtaaa
    11161 cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga aatcttttta
    11221 ctcgaagatg ggattgatgg ctggaaaaaa gcaggattac cagtagcggt aaataaatct
    11281 caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat attaatcggc
    11341 gttatactgg gttataccgt aaatagcggt ttcttcttat taagcggctt tgttggtgcc
    11401 gggttactgt ttgcaggaat tagcggtttt tgtgggatgg caaggttgtt agataagatg
    11461 ccgtggaacc aatgagcttg agaagcgacg ccggacgcgc cctagcagcg acatccggcc
    11521 tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc tgagcgattg
    11581 gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca tcggtgaatt
    11641 tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca gcagagctat
    11701 tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc caggtgctaa
    11761 ttttttcctg gcgctctgcc agttcacgct gctgctgttc ttcttcttca cgtctttctt
    11821 tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag aattcgcgag
    11881 ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac ataataaaga
    11941 aaacctttta acgccaaaac aaaaaag
//
LOCUS       DAZKFZ010000133        11906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000133 DAZKFZ010000000
VERSION     DAZKFZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1151..2239
                     /gene="yehL"
                     /locus_tag="ACHOQW_003256"
     CDS             1151..2239
                     /gene="yehL"
                     /locus_tag="ACHOQW_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP9574289.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            2250..4529
                     /gene="yehM"
                     /locus_tag="ACHOQW_003257"
                     /pseudo
     CDS             2250..4529
                     /gene="yehM"
                     /locus_tag="ACHOQW_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            4522..5658
                     /gene="yehP"
                     /locus_tag="ACHOQW_003258"
     CDS             4522..5658
                     /gene="yehP"
                     /locus_tag="ACHOQW_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9574290.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            5655..7658
                     /gene="yehQ"
                     /locus_tag="ACHOQW_003259"
     CDS             5655..7658
                     /gene="yehQ"
                     /locus_tag="ACHOQW_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9574291.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            7783..8244
                     /gene="yehR"
                     /locus_tag="ACHOQW_003260"
     CDS             7783..8244
                     /gene="yehR"
                     /locus_tag="ACHOQW_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP9574292.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(8286..8756)
                     /gene="yehS"
                     /locus_tag="ACHOQW_003261"
     CDS             complement(8286..8756)
                     /gene="yehS"
                     /locus_tag="ACHOQW_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9574293.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(8803..9522)
                     /gene="btsR"
                     /locus_tag="ACHOQW_003262"
                     /gene_synonym="yehT"
     CDS             complement(8803..9522)
                     /gene="btsR"
                     /locus_tag="ACHOQW_003262"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP9574294.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(9519..11204)
                     /gene="btsS"
                     /locus_tag="ACHOQW_003263"
     CDS             complement(9519..11204)
                     /gene="btsS"
                     /locus_tag="ACHOQW_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP9574295.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            11426..11491
                     /locus_tag="ACHOQW_003264"
                     /pseudo
     CDS             11426..11491
                     /locus_tag="ACHOQW_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11719..>11906
                     /locus_tag="ACHOQW_003265"
     CDS             11719..>11906
                     /locus_tag="ACHOQW_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9574296.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKT"
BASE COUNT         2929 a         3121 c         3002 g         2854 t
ORIGIN      
        1 atgcgtttca atttgcccgt tacttcaaca aatcaatcaa ccgtaacaga gccactaata
       61 actccagtac ggcaatgagc acggttaact tttgtgtcag cgtgtccatc cgctctcctt
      121 attaaggagc gcaacaccag caccttaccc ttacactgcc tgtcgatact ggtttacgta
      181 tgttggtacg aaacgttgct ctccggatca gggcaccgcc tgcggcacca cccgtccggc
      241 agccaggact taaaattggt ccgatccggc acaacctccc aacgggcagg attataaatc
      301 tcactcactg aattgcaatg gagtgtggat atggttataa tttattcgtt ggtacgaaat
      361 gttgcacaat cagcaagtta gcgtcactgt agtaaataaa agaacctgcc tcaccagcag
      421 gtttttttat tttctgcgat ctactttcca gatatttttt tgccaatagc ccaccataaa
      481 tattcacatc ccggtcaaat ggtgcgttga atataaaatc gatgaattta taaataaaaa
      541 tgagttatat gatgaattaa taaaaacagt aatcatttta ttaaaggggg aagcggtttt
      601 cccccttaga tgcgtttcaa tttgcccgtt acttcaacaa atcaatcaac cgtaacagag
      661 ctactaatag ctccagtaca gcaatgagca cggttaactt ttgtgtcagc gagtccatcc
      721 gctctcctta ttaaggagcg caacaccagc accttaccct tacactgcct gtcgatactg
      781 gtttacgtat gttggaccac gatgttgcac tccggatcag ggcaccacct gcagcacccc
      841 ccgtccggta gccaggactt gaaataggtt cgatccggcg caacgtccca acagtcagga
      901 ttataaatct cattatctta attgcaacgg ggtccagccg tggttataat tcgttcgttg
      961 gaacacgatg ttgcacaggc tgtggtgtta ggcctgaaaa tagtaaataa aagaacctgc
     1021 ctcaccagca ggttttttta tttactgtga tctgcttccc agatattttt cgctcaaaca
     1081 actaatgcgc caaacattta ttgcgcgtaa aatatcgttt atttcattaa tacatttcag
     1141 ggatgaatat atgtcaccgc agaataatca tctgcagcgt ccgcctgctg ctgtgttata
     1201 cgccgatgaa ctggcaaaat taaaacaaaa tgataacgca ccttgcccgc ccggttggca
     1261 gttaagtttg cctgcggccc gtgcttttat ccttggcgat agcgcgcaaa atattagccg
     1321 taaagtggtg attagtccct ccgctgtcga acgtatgtta gtcactcttg ccaccggacg
     1381 cggtttgatg ttggtggggg aacctggcac cgcaaaatct ctcctgtctg aattactggc
     1441 aacctctatt agcggcgatg ccgggttaac catccagggc ggcgcatcta ctaccgaaga
     1501 tcaaatcaag tacggctgga actacgccct gcttatcaat catggtccat caacggaagc
     1561 gctggtccct gccccgcttt accagggaat gcgcgacggc aaaatcgtcc gttttgaaga
     1621 gataacgcgt acaccgctgg aagtgcaaga ctgtctgtta ggtatgcttt ctgacagggt
     1681 gatgacggtg ccggaactca ctggtgaagc cagtcagctc tatgcgcgag aaggctttaa
     1741 tattattgcc actgccaata cccgcgatcg cggcgtcaat gaaatgagtg ctgcgctaaa
     1801 gcgtcgcttc gattttgaaa ctgtctttcc gattatggat ttcgcccagg agctggagct
     1861 tgtcgccagt gcttcggcgc gtttgctggc ccatagcggt attccgcata aagtgcctga
     1921 cgcggtactg gagttactgg tccgcacctt ccgcgatctg cgtgccaacg gcgaaaagaa
     1981 aacgtcaatg gatacactaa cagcgattat gtccaccgct gaagccgtga acgtggccca
     2041 cgctgtgggc gtccgcgcct ggtttttagc gaatcgcgcg ggggagcctg ccgacctggt
     2101 ggactgtatt gccggaacca tcgtcaaaga taacgaagaa gatcgcgctc gtctgcgccg
     2161 ttacttcgaa cagcgtgtcg ccacacataa agaagctcac tggcaggctt attatcaagc
     2221 ccgccaccgc ctgccgtgag gaaagatgca tgagcgagcc gttaattgtc ggcatccggc
     2281 atcatagtcc ggcctgcgcc cgactggtga aatcgttaat cgaaagccag cgaccacgat
     2341 acgtgttgat tgaaggcccg gctgatttta atgaccgggt agatgaactg tttttagccc
     2401 accagcttcc ggtggctatt tacagttatt gccagtatca ggacggtgct gctcctgggc
     2461 gcggcgcctg gacgccattt gccgaatttt cgccggagtg gcaggcgcta caagccgcac
     2521 gtcatattca ggcacaaact tactttatcg atttgccttg ctgggcgcag agtgaagaag
     2581 aggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt gccacccgca
     2641 tggataacag cgataccctg tgggatcact tgttcgaaga tgaaagccag caaactgcat
     2701 taccctctgc actggcgcac tattttgccc aactgcgggg cgatttcccc ggcgatgcac
     2761 tcaatcgtca gcgcgaagcc tttatggctc gctggattgc atgggcagtg cagcaaaata
     2821 atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc ggcactggca aaaatgtggc
     2881 gcgaatgccc gcaggacatt aacaagccag aattgccctc gctggcagat gccgttacag
     2941 gttgttatct cacaccctac agtgaaaagc gccttgatgt gctggcagga tacctttcag
     3001 gaatgcctgc cccggtctgg caaagctggt gctggcagtg gggcttacag caggcgggag
     3061 aacaactgct aaaaacggtt cttacccgtt tgcgccaaca ccacttgcct gcttcgaccg
     3121 cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc ggatataaac
     3181 taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa gaagccctga
     3241 acgcgccgtt gccgtggagc tatcgcggcg ttattcatcc cgataccgat ccgattctgc
     3301 taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct tctacgccac
     3361 aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc tctctttcgg
     3421 cggagcttac cttaaatcgc tttaacccca atggactagc gcaaagtcag gtgttacatc
     3481 ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca ctgacacttg
     3541 caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat gcggcattga
     3601 ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa ttagaagccg
     3661 atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag gcggcgttag
     3721 cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta atcgcctagg
     3781 aaaatcaatt tgccgaaatg ggcccggcgc tggaagtgct atatgcctta tggcggctgg
     3841 atgaaattag cggtatgcaa ggtgcgcaga tatcacaaac gacgttatgc gcggctatcg
     3901 atcgcacgct gtggctgtgt gaatctaacg gcagaccgga tgaaaaggag tttcacgctc
     3961 acctgcatag ctggcaagcg ctttgccata ttctgcgcga tctacatagc ggcgttaatt
     4021 tacccggcgt ttctctttct gcggcggtag ccttactgga gcgacgcagt caggcaatcc
     4081 atgccccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg gaacatccca
     4141 acgctagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca caatccggcg
     4201 aagcgctgca cggtttgctg gcgctggccc gccatcaact ggcctgtcag ccggcattta
     4261 tcgccggttt cagcagtcat ttaaatcaac caagtgatgc cgattttatc aatgccctgc
     4321 ccgatttacg cgcggcgatg gcctggctac cgccacgaga acgcgggaca ctggcgcatc
     4381 aggtgcttga acattatcaa ctggcgcaac ttcccgtttc ggcgctgcaa atgccgttgc
     4441 attgcccacc acaagccatt gcacatcatc aacaactcga acagcaggca ctggcatcgc
     4501 tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac ccgtgagcta
     4561 caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg gctggatgac
     4621 aacgcccggc agatagacca cgcgctggag tggctgtatg ggcgcgatcc tgaacggctc
     4681 cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccacccc tgagtggatc
     4741 aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag cgatgccgta
     4801 ctgcgctacg gcattgaaga tgtggtgacg aatctcgacg tgctggaacg tatgcagcct
     4861 tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaaccc cgaagtactg
     4921 gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg actggcaaag
     4981 gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatc tattccactg
     5041 gcgcgagact ttgatttcaa aagtactctt cgcgccaatc tgcaacactg gcacccgcaa
     5101 cacggcaagt tgtatatcga atccccccgc tttaacagcc gaattaagcg ccacagtgaa
     5161 caatggcaac tggtcttact ggttgatcta agcggatcga tggtcgattc ggtgatccac
     5221 tctgcggtaa tggcggcctg tttgtggcag ttacccggca ttcgtaccca tctggtggcg
     5281 tttgacacca gtgtcgttga tctcacggca gacgttgccg atccggtaga gttattaatg
     5341 aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg gcggcaactt
     5401 attgaacaac cagcaaaaag cgtcattatc ctcgtgagtg atttttatga agggggttca
     5461 tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa agtgctgggg
     5521 ctggcagcgc tcgacagcac cgcaacgcct tgctatgacc gcgatatggc ccaggcgctg
     5581 gttaatgttg gcgcacaaat agccgcaatg acaccgggtg aactggctac ctggcttgcg
     5641 gagaatttgc agtcatgaat tcactacgtc cggaattatt agaactcacg ccgcaggccc
     5701 tgacggcgtt aagcaatgcc ggttttgtta agcgcagcct taaggaactg gaaaatggca
     5761 acgtcccgga aatcagccat gagaacggcg ctttaatcgc caccttcagt gacggtgtcc
     5821 gtacccagct ggcgaacggc caggcactga aagaggctca gtgcacctgc ggggctagcg
     5881 gcatgtgccg tcatcgcgtg atgctggtgt taagttatca gcgactttgt gccaccaccc
     5941 ggcccacgga aaaagaagaa gagtggcagc ctgcaatctg gctggaagaa ctggctaccc
     6001 tgcccgatgc cacccgcaaa cgtgcacagg tgctggtcgc taaaggcatc accattgagt
     6061 tgttctgtgc gcctggcgaa attccctcag cccgcttacc gatgagtgat gtgcgttttt
     6121 attcccgcag cagcattcgc ttcgcccgct gtgattgtat tgaaggcaca ctttgcgaac
     6181 atgtcgtact ggctgtgcag gccttcgtcg aggccaaaac tcagcaagcg gaatttactc
     6241 atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgacccg tttgccagcg
     6301 aagaaggcaa cgcgtgtcgt caatatgttc agcaattaag ccaggcatta tggcttggcg
     6361 gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag caagcggcgg
     6421 aacgctgcaa ctggcgctgg gtgagtgaat cgctacggca actgcgcgcc agcgttgatg
     6481 ccttccacgc ccgcgccagc cactatcatg caggagaatg cttacgtcag cttgcggcat
     6541 taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtgtt ggtgaagttc
     6601 ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca aagcttgatc
     6661 atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat tatggtttac
     6721 gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc agttggccgc
     6781 gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt caggctggcg
     6841 cactggcggg tgggcaaatt gtttcacaag cagcaaaacg cagtgccgat ggcgagctgc
     6901 tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat gcctggcgaa
     6961 tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat ttacgccagc
     7021 gtcccccctc ctgcatacgg cctcttaatc aggtcgataa cttatttatt ctgccggtcg
     7081 ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg caggtaatca
     7141 gcggcgaagg tgaagataat ctgctgacgt tatcactgcc agcctcagcc agcgcgcctt
     7201 atgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg tgtctggttt
     7261 ctggctttgt cagttttgtt gatgggcaat tgacactgga accacaggta atgatgacaa
     7321 agactcgtgc ctgggcgctg gacgcagaaa ctgcgcctgt ggtggcatca ctaccttctg
     7381 ccagcgttct gcctgtgccg tctactgctc atcagttgct gatgcgctgc caggcattac
     7441 ttattcaact gctccataac ggctggcgct atcaggaaca gagtgctatt ggtcaggcag
     7501 agttgctggc gaatgacctc accgcggtgg gtttttatcg gctggcacat gtattgggac
     7561 aatttcgtaa tacagaaagc gaggcacggg tagaagcaat gaataacggt gttttgctgt
     7621 gcgaacaatt attccccctg cttcagcaac aaggatgaaa tagtgctttt tactaagagt
     7681 tctactccag ttccggactg ctcacgccac ggtattaggc atatcctata tagcccctgg
     7741 tgagagtcac cagttccttg attaaataaa atggagtttt acatgaaggc tttcaataag
     7801 ctgttttccc ttgttgttgc atctgttctg gttttttctc ttgctggctg cggtgacaaa
     7861 gaagaatcga agaaattcag cgccaatctg aacggcactg aaattgccat tacctatgtc
     7921 tacaaaggtg acaaggtgct taagcaatct tctgaaacca aaattcaatt tgcctccatt
     7981 ggtgctacca ccaaagaaga tgctgccaag acacttgagc cgttaagcgc caaatacaaa
     8041 aacatcgcgg gtgttgaaga aaaattaacc tataccgata cctacgcgca ggaaaacgtg
     8101 actatcgata tggaaaaagt ggattttaaa gccctgcagg gtatttcagg aatcaacgtt
     8161 tctgctgaag atgccaaaaa aggtatcact atggcgcaaa tggaactggt gatgaaagcc
     8221 gctggtttta aagaagtgaa ataatctgtc ggcggccatg tttgcgcatg gccgccatac
     8281 ccgtcttagc ttttcttcac atgctggcgc gctgccagtc cacgcagaaa ataacgtaaa
     8341 aattgatcgc cgcattcgcg gaagttttta tgatccggtg cacgcatcat cgccgtaatt
     8401 tccggcatcg aaacgcggaa ctgctgttcg gtgaggatcg ccagaatgtc atcggttttc
     8461 agcgaaaacg cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg acgttccggc
     8521 tccagtgccg gagcagactc atccttgccg cgtttttcat aaatcaggcc attgaggaat
     8581 gacgacaaaa caatgtccgg acaacgctga aaaccctctt cgtcttcttt acgtagccag
     8641 acggcgatct gttccgcggt ggcttcgaca ttacccagcg ccagtatacg caccaggtca
     8701 ttattattgg ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact tagcatgagg
     8761 ccttcaggtg ttgatgaggc aaaaagccat tttagcagtc ttttacaggc caatcgcctc
     8821 ttttaagctt ttcagataac ggcggctgac cggcacggtt aagccattac gcaaaatcaa
     8881 ctcggcctga ccgttatctt ccagacgaat ctcctgtaaa tgcgcgaggt taaccagata
     8941 ctgacgatgg cagcgcagta gtggtgtacg actttccagg gtacgtaatg tcaattcggt
     9001 aaagccctct ttcccttcgt ggctggtaac gtagacaccg ctcatccgac tgctgacaaa
     9061 tgccacatct ttcatttgca gcaaataaat ccgactatgc cccgtacaag ggataaattt
     9121 cagcgcctgt tgattttccg gtaacagcga aacatcctgc ttgctgcgct cctgacgcaa
     9181 tcgcgccagc gttttctcca gtcgcgcttc atcaattggc ttcagcagat agtcaaaggc
     9241 atgttcttca aaggctttaa tagcgtattc gtcaaacgcg gtgagaaaaa caatatacgg
     9301 acgatgttcc gggtcgagca tccccaccat ttccagacca ctgatgcgcg gcatctggat
     9361 atcgagaaac agcacatccg ggcgcagttt atgcaccgcg ccgatccctt ccacggcgtt
     9421 tgaacactca ccaacgattt caatatcgct ctgctcctgc aaaaatacgc gcaggttctc
     9481 ccgtgctaac ggttcatcat cgacaattaa gactttaatc atgcctcgtc cctccatggt
     9541 agtcgtaacg ttattcgggt gtaactatca ggctcacagg cgacgcttat cccatagtca
     9601 tcgccaaacc gttcacgtaa acgcttatcc accagattca tccccagccc actggcattg
     9661 gttaccggtt gatacaaacc ggcattgtct tcgatctcca gcatcaaatg ttgcccctca
     9721 cgtcggacgc tgattgccac tcgccctgta tccagcagtt gtgatgtccc atgtttaatg
     9781 gcgttttcca ctatcggttg cagggtaaac gcgggcaatt gctgctggga taattcttgc
     9841 ggaatagcaa tgttgacctg caaccgcgac tggaagcgcg ccttttcaat ttgcagataa
     9901 gcattcacat gttcaatttc gtcggcgaga gtaacaaact ccgaaggccg ctttaagttt
     9961 ttgcggaaaa aagtggaaag atactgcacc agctggctgg cctgttcgct gtcgcgacgg
    10021 atcaccgctt taatggtgtt aagcgcatta aacaaaaaat gggggttcac ctgggcgtga
    10081 agcagtttga tctctgactg ggtgagcatc gctttttgcc gctcatattg cccggcgagg
    10141 atctgcgccg aaagcagttg cgcaatcccc tcgcccagcg tgcggttgat tgaactaaat
    10201 aaacggtttt tggcttcata caatttgatg gtgcccatca cccgctgatt ttcaccacgc
    10261 aacggaatta ccagcgtcga ccccagtttg cattgcggat gcaaagagca acgataaggt
    10321 acttcgttgc catcagcgta gaccacttca ccggtttcaa tcgcttttaa agtataagtt
    10381 gaagaaatcg gtttgccggg taaatggtgg tcgtcaccaa ttccggtaaa ggccagcaat
    10441 ttctctcgat cggtaatcgc gactgcacca atatccagct ccagatacag cacctgagcc
    10501 actttcatgc tgttcacttc gttaaacccc tggcgcaaaa tgccttccgt cgaggctgcc
    10561 actttcagcg cagtggcaga aaaagccgaa gtgtattttt caaacatcgc gcgtttatcg
    10621 agcaatatac gcataaacag cgccgcgccg acggtattgg tgaccatcat tggcgcagca
    10681 atattactca ccagacgcac cgcatcttca taaggtcggg cgatcgccag gatgatcagc
    10741 atttgcacca tttcagcgac gaacgtgacg gcaccagcgg taatggggtt aaagacttta
    10801 tcagtgcgcc cgcgacggat caggatgctg tgtaccaggc cgccgagcaa tccttcaacg
    10861 atggtcgaga tcatgcaact tagcgcggtc atgcccccca tcgaatatcg atgtaacccg
    10921 ccggtcaggc caaccagccc accgacgacc ggaccgccga gtaagccgcc cattaccgcg
    10981 cctatcgcac gggtattggc aatagaatcg tcaatgtgca acccaaacca ggtgcccatg
    11041 atgcagaaga tggaaaagac gatgtagcag agaaatttat gcggcagacg aaccgtgacc
    11101 tgcattaacg gtatgaataa tggcgtttta ctcattaacc acgcaatgac taaaaaaacg
    11161 cacatctgct gaagcagcag caacaccaga ttaaaatcgt acatacccgc aaaccacaca
    11221 tccctttaaa acgcgtaaca tacattgcct gcgtttaact ttctttgaac tcttgcagaa
    11281 aaatgagaat tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa atcaccctat
    11341 aaatgcacaa aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa agttaaaccg
    11401 ggacctcgcg agcaagggtg agacgatggc gctttacaca attggtgaag tggcgttgct
    11461 ttgtgatatt aaccctgtca cgttacgcgc gtgacgcaac atttttaaaa atcgacgaag
    11521 atcgtccagg agcgccgttt ttcaagggtt ggatagatga ttcgtttacc atgtcgatga
    11581 acttcgacga atttcgccga taaaaagtgt cccatacatg ccccattgat gccccataaa
    11641 caatatcagg aattgacact ggttatccat acagataaaa ataacacctg tacataacta
    11701 cagttacagg agggtgttat gcgtgttgag atctgtatcg ctaaagaaaa aatcactaaa
    11761 atgccaaacg gtgctgtgga tgcgttaaag gaagaattaa cccgacgcat cagtaaacgt
    11821 tatgacgatg tagaggtgat cgtaaaagcc accagcaacg atggcctttc tgttacgcgc
    11881 accgccgata aagattcagc taaaac
//
LOCUS       DAZKFZ010000134        11852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000134 DAZKFZ010000000
VERSION     DAZKFZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            81..1232
                     /gene="yiaY"
                     /locus_tag="ACHOQW_003266"
     CDS             81..1232
                     /gene="yiaY"
                     /locus_tag="ACHOQW_003266"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP9574297.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1362..2657
                     /locus_tag="ACHOQW_003267"
     CDS             1362..2657
                     /locus_tag="ACHOQW_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP9574298.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            2765..4303
                     /gene="aldB"
                     /locus_tag="ACHOQW_003268"
     CDS             2765..4303
                     /gene="aldB"
                     /locus_tag="ACHOQW_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP9574299.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4522..4665)
                     /locus_tag="ACHOQW_003269"
     CDS             complement(4522..4665)
                     /locus_tag="ACHOQW_003269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574300.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            4848..5171
                     /locus_tag="ACHOQW_003270"
     CDS             4848..5171
                     /locus_tag="ACHOQW_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9574301.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            5177..6313
                     /locus_tag="ACHOQW_003271"
     CDS             5177..6313
                     /locus_tag="ACHOQW_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9574302.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(6310..7284)
                     /locus_tag="ACHOQW_003272"
     CDS             complement(6310..7284)
                     /locus_tag="ACHOQW_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9574303.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            7408..8148
                     /locus_tag="ACHOQW_003273"
     CDS             7408..8148
                     /locus_tag="ACHOQW_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP9574304.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(8278..10248)
                     /locus_tag="ACHOQW_003274"
     CDS             complement(8278..10248)
                     /locus_tag="ACHOQW_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFP9574305.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(10259..11659)
                     /locus_tag="ACHOQW_003275"
     CDS             complement(10259..11659)
                     /locus_tag="ACHOQW_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9574306.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
BASE COUNT         2935 a         2894 c         3108 g         2915 t
ORIGIN      
        1 aattaataat aatgatcatg ttttcgaatg aaaatcctca gtaagctgcc cacccttttt
       61 tacactttca ggagtgtgtt atggcatctt caactttctt tattccttct gtgaatgtca
      121 tcggcgctga ttcattgact gatgcaatga atatgatggc agattatgga tttacccgta
      181 ccttaattgt cactgacagt atgttaacga aattaggtat ggcgggcgat gtgcaaaaag
      241 cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct aaccccacca
      301 cggaaaacgt cgccgcaggt ttgaaattac ttaaagagaa taattgcgat agcgtgatct
      361 ccttaggcgg tggttctcca cacgactgcg caaaaggtat tgcgctggtg gcagccaatg
      421 gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag ctgccgatga
      481 tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc atcatcactg
      541 acgaagcgcg tcatatcaaa atggcgattg ttgataaaca tgtcactccg ctgctttctg
      601 tcaatgactc ctctctgatg attggtatgc cgaagtcact gaccgccgca acgggtatgg
      661 atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg atcactgacg
      721 cttgtgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc gttgaagatg
      781 gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca gttcctcgcc ggtatggcgt
      841 tcaataatgc ttctctgggt tatgttcatg cgatggcgca ccagctgggc ggtttctaca
      901 acctgccaca cggtgtatgt aacgccgttc tgctgccgca tgttcaggta ttcaacagca
      961 aagtcgccgc tgcacgtctg cgtgactgtg ccgctgcaat gggcgtgaac gtgacaggta
     1021 aaaacgacgc ggaaggtgct gaagcctgca ttaacgccat ccgtgaactg gcgaagaaag
     1081 tggatatccc ggcaggccta cgcgacctga acgtgaaaga agaagatttc gcggttctgg
     1141 cggctaatgc cctgaaagat gcctgtggct ttactaaccc gatccaggca actcacgaag
     1201 aaattgtggc gatttatcgc gcagcgatgt aatcatcatt tccacaacgg ctggcaaatt
     1261 gtcagccatt ttttcaacta tctcaaaact tgcttttttt gagatagttt ctctttcctg
     1321 ctatacacta tctcaatatt gcgctttttt gagataagca aatggctatc aaatcacctc
     1381 ccacgctgat tcctttatca cacttatcgg gtgaggaact ccaggcccac ctgcgcttta
     1441 accgggttac cgatgaaaaa ggacgttatc tgccttttga tgagttgcag taccgcatca
     1501 aaaaaggaga aaacgtcgat gtggcgtgga cgcttacccg tctggcgcgc aacgcggcga
     1561 tccaacgaat taattattgc aatgaagcgg gtgaacaggc cggattcaac attaccccgg
     1621 tgattgcaga agcgtgcgag cttgtcgaca aacgtgctac cgcgctggcg ctgaaagatc
     1681 agactgaacg gttacgcggg gccggagccg agttaagcca gttacgcctg gaagagccga
     1741 tcaccagctc ccagctggaa ggtgcgaata ctacaacgct ggtggcgaga aaaatgctgg
     1801 aaacaggacg gtcgccacgc actgaagacg agcatatgat tgcgggaaat gcccggctga
     1861 tggcggaaat tccgcactta ctcgcagaac cgctgacacc tgcgcttatt cgccagttac
     1921 atgccattgg tatgggtgga atcaatgacg cgaaatatcg tccgggagaa tttcgtgaaa
     1981 cagatgatgt tgtgatcgcc gactatgatg gcaatatagt gcatcaaccg cccgctgcgg
     2041 cattgttacc ggaacggctg gaaaaagttt gccagtggtt aaacagccat gaagggtata
     2101 ttcatccgtt ggtccgtgcc tgcattctgc attttatgct ggcccatgaa catccgtttc
     2161 gggatggcaa cgggcgcacc agtcgggcgc tgttttactg gtatatgctg aaatcgggct
     2221 acgatgtctt taaatatatt tccatcagcc gtttgcttca tgctgcaccg gtcaaatatg
     2281 ccgccagcta ccagtacact gaatcagatg gtatggatct gacttatttc cttgaatatc
     2341 aggcgggtgt cattaaacgt gctctgcaaa actggcagca gcatatcgat gaaattacac
     2401 aacgaagcgc aaaactcgat agtgtgctgt ttagctctgg tgtgcttaaa cgcctgaatc
     2461 cgcgccaggt aacgctcctg aatgtgatgc tcgccaatcc ggggaaagag tacacagttg
     2521 ctgaaattag cgcgagcctg ggcgtctctg ataatacggt tcgagctgat ttacggacaa
     2581 ttgttaaaga agggtttgcg caagagaaga aaattaatga ccagcaagcc gtgtacttcg
     2641 cgcattatcc gctgtaaccc ttgcccgtaa attcgtgata gccgtcgaaa agctgttacc
     2701 gactggcgaa gatttcgcca gccacgtcta cccttgttat acctcacacc gcaaggagac
     2761 gatcatgacc aataatcccc cttcagcaca gattaagccc ggcgagtatg gtttccccct
     2821 caagttaaaa aaccgctatg acaactttat tggcggcgaa tgggtagccc ctgccgatgg
     2881 tgagtattac cagaacctga cgccggtgac cgggcagctg ctgtgcgaag tggcgtcttc
     2941 gggcaaacga gacatcgatc tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc
     3001 gcacacctcg gtgcaggatc gcgcggcgat tctgtttaag attgccgatc gaatggaaca
     3061 aaacctcgag ctgttagcga cagctgaagc ctgggataac ggcaaaccca ttcgcgaaac
     3121 cagtgctgcg gatgtaccgc tggcgattga ccatttccgc tatttcgcct cgtgtattcg
     3181 ggcgcaggaa ggtgggatca gtgaagttga tagcgaaacc gtggcctatc atttccatga
     3241 accgttaggc gtggtggggc agattatccc gtggaacttc ccgctgctga tggcgagctg
     3301 gaaaatggct cccgcgctgg cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac
     3361 cccgctttct gtactgctgc taatggaaat cgtcggtgat ttactgccgc cgggcgtggt
     3421 gaacgtggtc aacggtgcag gtggggaaat tggcgaatat ctggcgacct cgaaacgcat
     3481 cgccaaagtg gcgtttaccg gctcaacgga agtgggtcaa caaattatgc aatacgccac
     3541 gcaaaacatt attccggtga cgctggagct gggcggtaag tcgccaaata tcttctttgc
     3601 tgatgtgatg gatgaagaag atgccttttt cgataaagcg ctggaaggct ttgcactgtt
     3661 tgcctttaac cagggcgaag tttgcacctg tccgagtcgt gccttagtgc aggagtcaat
     3721 ctacgaacgc tttatggaac gcgccatccg ccgtgtcgaa agcattcgca gcggtaaccc
     3781 actcgacagc gtgacgcaaa tgggtgcgca ggtttctcac gggcaactgg aaaccatcct
     3841 caactacatt gatatcggta aaaaagaggg cgctgatgtg ctcaccggcg ggcggcgcaa
     3901 gctgctggaa ggtgaactga aagacggcta ctacctcgaa ccgacgattc tgtttggtca
     3961 gaacaatatg cgcgtgttcc aggaggagat ttttggcccg gtgctggcgg tgactacctt
     4021 caaaacgatg gaagaagcgc tggagctggc gaacgatacg caatatggcc tgggcgcggg
     4081 cgtctggagc cgcaacggta atctggccta taagatgggg cgcggcatac aggctgggcg
     4141 cgtgtggacc aactgttatc atgcttaccc ggcacatgcg gcgtttggtg gctacaaaca
     4201 atcaggtatc ggtcgcgaaa cccacaagat gatgctggag cattaccagc aaaccaagtg
     4261 cctgctagtg agctactcag ataaaccgtt ggggctgttc tgatgtaaga agctggtcgc
     4321 atcgggtatt aattgcctga tgcgacgctt acgcgtctta tcatgcctat ggggacctga
     4381 ccgtaggccg gataaggcgt acacgccgca tccggcataa acaaagcgca cgttgttaca
     4441 atttacgttt taaacctacg tgtgcaataa cccagactac gccaacaaaa gtgcgtttct
     4501 ggggcgtgtt ttgccaggag tttaatctcg cgtttcgatg gtgagcgggc aaaccagacc
     4561 gtaaatcacc attggtattc cgaggacaat cagcaataaa cggtagatta ttgaaggttt
     4621 aatacgctta atctgcattg tggatgtttt actgaagtta ttcattagtt tggctgatag
     4681 ttccattatt tcaatatgat aagcctcgac ctgttaaaaa gccacgcagc acacttcaga
     4741 aagtatataa aaaacaatat gtcgttatat aaataaggaa attgcattgc atcatggcat
     4801 ttcataatat gcgttaattg ttctttttta tattctctga gggaaatatg ttcctggact
     4861 attttgcact gggagtgctt atttttgtat ttctggtgat attctatggg attattattt
     4921 tacatgatat tccctacctg attgctaaaa aacgtaatca tcctcatgcc gacgccattc
     4981 atgttgctgg ttgggtgagt ctttttacgc tgcatgttat ctggccattt ttgtggattt
     5041 gggccacgct ttaccgcccg gagcggggat ggggaatgca aagccatgat tcatccgtta
     5101 tgcaactgca acagcgcatt gccgggctgg agaaacagct cgccgacatc aaatcctctt
     5161 ctgccgagta atacttatgg atttactgat tattttgacc tatgtggctt ttgcatgggc
     5221 aatgtttaag atcttcaaaa ttcccgtaaa taaatggacc attcccacag cggccctggg
     5281 tggaatattt attgtcagcg gtctaattct gttaatgaac tataaccatc cgtatacctt
     5341 taaagcgcaa aaagcggtta tttctattcc tgttgtccca caggtgacag gcgtggtgat
     5401 agaagtgacg gataagaaaa atacgctgat taaaaaaggt gaggtgctat ttcgactgga
     5461 cccgacgcgt tatcaggcgc gggtggatcg gctgatggcg gatatcgtta ccgcagaaca
     5521 taaacagcgg gcgttgggcg cagagttaga tgagatggcg gcgaatactc agaaggcaaa
     5581 ggccacgcgg gataaattcg ctaaggagta ccagcgttac gcacgcggca gccaggcgaa
     5641 agtaaacccg ttttcagaac gcgatattga tgtggcacgg caaaattatc tggcgcagga
     5701 agcctccgta aagtcatcgg cggcggaaca aaaacagatc cagagccagc tggatagcct
     5761 ggtgttgggt gaacattctc aaatcgccag cctgaaagcg cagctcgcgg aagcaaaata
     5821 taaccttgag cagacgatag tgcgtgcgcc gagcgatggt tatgtgaccc aagtgctgat
     5881 tcgtccgggt acctatgccg cgtcgctgcc gctacgtccg gtgatggtgt ttatacccga
     5941 tcagaaacga caaatcgtgg cgcagttccg tcagaactcc ttgctgcgcc tggctcccgg
     6001 cgatgatgcg gaagtggtat ttaacgctct gccaggcaaa gtgttcagcg gtaagctggc
     6061 agccattagt ccagctgttc ccggcggagc ttatcagtcg accggcacct tacagacgtt
     6121 aaacacagcg ccgggttcag atggcgttat cgcgaccatt gaactgaatg agcacactga
     6181 tttgagcgct ttaccagacg gtatttacgc tcaggtggcg gtctactctg atcatttcag
     6241 ccatgtctcg gtgatgcgca aagtgttatt acgcatgacc agctgggtgc attaccttta
     6301 tctcgatcat taatcttcgc tttccgccgc ttgttcaggc cgcagttgct gcgactgagc
     6361 aagctggtgg cgaatttcac gcagcatttc atccagcaca tactggaccc gccagggctg
     6421 gtattcccgt ttacggaaga tagccaccag atccagccac cattcatacg ggtgctgaaa
     6481 gcagggcacc agcgtgccgt cttgcagatc gctctgcaca cttttgtctg gcgcaaacac
     6541 aatccccagg tgatttcgtg ccagctccag cgccgattgc gtgttatcgc aaacgtaatt
     6601 tcccttcacg cgataatccc gcacctcttt actcccggcg acgttgaagc gccagatatt
     6661 cgcgtcgtcg atcatcatcg agtcgatcaa aatacaggag tgatgttcga gttcgtcagg
     6721 acggctaata ggatgtttct caagatagcg ctggctggca taagcggtta cggcatattg
     6781 ggtaataaaa ctggcaacca gcgattcatc ttttggtggg gcatagctga ttaaaacatc
     6841 acaatcatca ggaaattcga cgccttcata aaaggcatta cgctcaagat tgcaggtttt
     6901 tagcgataag gtgatatcac cgatatcttt aattttatcg attacatgtt tggataaata
     6961 ggtaattatg ccggttgggg cgtagatggt gacccgacca cgtttctcat gcttataatc
     7021 tgcaataaaa ttattaagct gctcgtttct gtccagcatg tcgttgatgt acggtaatag
     7081 cgcggtgcca aaaagtgtga gcatcagttg tcgggtggtt cggtcaaaga cttttaaacc
     7141 cacttttgat tcaaaatcag caagatattt gctgacgttg gcctgtgcga tgccaagtac
     7201 agtcgcggca tggctgatat tttcactggc agcgattacc gagataattt ttaactcccg
     7261 gtacttaagt tgtaattttg tcatcgcaac agcccaatta tatataattt tatatattac
     7321 tatataaata tgaatcttgc accggagaaa ttgtaagcat tactatgccg actctttttt
     7381 atcttatttt aaaatggaat accggacatg ttaattaacc gcaatattgt ggcgttattt
     7441 gcgttgcctt ttatggcaag cgcaactgct tctgaattat ccattggtgc tggtgcggct
     7501 tataatgaat cgccttatcg cggttataat gaaaatacaa aggcaattcc gctgattagt
     7561 tatgaaggtg attcttttta tgttcgtcag accacgttag gttttattct gtcgcaaagt
     7621 gaaaaaaatg aacttagcct gaccgcatcc tggatgccgc tggaatttga ccctgccgat
     7681 aatgacgatt atgccatgca acagcttgat aagcgtgata gtacggctat ggcgggggtt
     7741 gcctggtatc accacgagcg ttggggaacc gtgaaagcct ctgcagctgc ggacgttctg
     7801 gataacagca acggctgggt gggggagcta tcggtattcc acaaaatgca gataggtcgt
     7861 ctgtcgctga cacctgcgct gggcgttctc tattatgacg agaatttcag tgactattac
     7921 tatggcattt cagagagtga gtcccgtcgt agcggtctgg caagttattc cgcgcaggat
     7981 gcctgggtgc cctatgtcag cctgacggca aaatacccga taggagagca cgtcgtattg
     8041 atggcgagcg caggatacag cgagctgccg gaagagatta ccgacagccc gatgattgat
     8101 cgtaatgaga gttttacctt tgtcaccggc gtgagctggc gtttttaatt caccggtgga
     8161 tgtcggtgcg gctggcctac gctctcgcac cgacttgtat gcctgataag acgtgttaag
     8221 cgtcgcatca ggcaatgtgc tccttacgtt ggatgcggcg cgagtgcctt atccaaccta
     8281 cggatggcaa tgcttttcct cattcaccca gatccgcatt tcgccttcac cccggttggc
     8341 ccagctaaac cacgggataa aggtcagagt ttgcgtctgg cgtttggctg gcgcgctgtc
     8401 gtaatgccac agcggttgct gctctggatt gctctgttca taccggtaac ccggtgcctg
     8461 gattaagatc ttatggctaa acaatccctt gccttcaaat gtcgtaaatg gcgcatcggt
     8521 gggcagccac agattatgca gtgactcgcc gttgtcggcc ttttccaggc aatacaccag
     8581 cgggccgcgc tgaatcgcca cttttccggc gacgtgacgc accagcgggt taccgtaaac
     8641 gcggcgtacc ggcatcggca aagtcagatt cagcgtatcg ccctcctgcc attcgcgggt
     8701 aatgtgcaaa taccctttac gaatatcctg ctcgacctct tccccattca atatgatctg
     8761 cggctgtgtg caccagtccg gcagacgtaa agccagcgta tgacgtaccg gctggggcga
     8821 ttcaaccgca atcgtcacct gctcctgcca cggatagttc ccgctaaccc gcaggcgcag
     8881 cgtgccattt tctaccggca cttccatgct gtttcctgcg tagatgttga tatacaacgc
     8941 atcttcacgc ggcgtgtaga gataatgacc aatcgaggtc agcacgcggg cgatatttgg
     9001 cggacaacaa gcgcagccaa accaacgctg gcggatcggt ttaacgtgat cgtagatatg
     9061 gttgaatttc agcgattttg gatgtacttc cagtggattc acatagaaga aatgtttgcc
     9121 atccaatgcc atgccgccga gcacagtgtt gtacagtgcg cgctccatca catcggcata
     9181 ttgactgtcg ccttccattt ccagcattcg tcgggcgaac atcatcaggc cgatggaagc
     9241 acaactttcg gcgtaaaccg tgtcattcgg cagatcgtaa tcgctgctga acgcttcgcc
     9301 gctgctttgt gagccgatgc cgccggtaat atataactga cgctgggcca tattgttcca
     9361 cagccgcagg cagtcctgac gcttgctgtc atcgtgactt aaacgcgcga gatgcgcgac
     9421 gccggtcatc agatagacaa aacgtaccgc gtgaccgatg gcggtttgct gttgcgcaag
     9481 gggcaaatgt gcctggctgt aggctttgtc tttcaccatc cacgccgggc cgtaggtgtg
     9541 ccagtgcgat gtctgcccgc gcttttcata ttcctggtcg taatagtgcg gttgcgcacc
     9601 acgctgttcg acaaaatagt tcgtcagcgc caggtagcgc ggctcttcag tcacttcata
     9661 caggcgcatt agtgccagtt caatttccgg gtgaccagga taaccgtgta acttactttc
     9721 atctggacca aatacgctgt cgatatgatc ggccaggcgg caaacgaccc ccagcaagcg
     9781 ccgttttccc gtggcctgga agaaggcgac tccggcttca atcagatgac ctgcgcagta
     9841 aagttcatga cactccgcca gattgctcca gcgttcttcg ggtgctttta ccgtaaagta
     9901 agtattgaga tagccgtctt cgcattgggc ggaggcgatc agttcgatta cctcgtcggc
     9961 ggttttttcc agttcggcgt ccggcttctg gcacagcgac caggctaccg cttccagcca
    10021 tttggcgacg tcgctgtcct gaaacaccat cccgtaaaat tcaccctcct gaagtccggc
    10081 ggcaatgcga aagttttcaa tcgcatggct gggttccgct tctgggatac gatcgttcaa
    10141 ggcatcccac tgataaggaa tcaccacgtc gcggaccagt tgttggtact gaccgaggaa
    10201 cggatcgctg accgtcagtt tatgcagatc gacttccgaa atgttcatct tgtgctcctt
    10261 aaggctgtac atgacgctga cgcaggtcgg tagctatgcg tgacatcata ggattattga
    10321 gtttgcaaaa gcggatcgcc cctgccagca gcaggtggaa tagcgcaggc agtaatgttt
    10381 ccatcgccgc gatcccgcca agcgaactgg cggtctggtt ttccacgcca ggctggtagg
    10441 cgacaaaaat aaacagcagg ctgatgatcc cggcgctgga ggcccaggcc agcttgatga
    10501 aaaacagatt gaaggcgaag ttcatgccgg aagaacgtac gtgggttttc cactcgccgt
    10561 agtcatcggc aaaggccatc aatgagaagt gcagtggcaa tgtgaagcca agaatcacgc
    10621 cattaccgag gatcaccgcc agccacagcg tttgataagc cgggccggag ggcaggaacc
    10681 acatcaacac cgccagtgca gcgaggagca ggttggtgta gtaataaatt ttgacggtat
    10741 cgaaacgccg cgttaacggg ctgacaatca ccgagccgat aatggaggcg aaggtgacca
    10801 tggtgaagaa cagcgacgtg tagcccgtgc tgccttgtaa gacgtaggta atgaagtaca
    10861 tatacccacc gccgcgaatg ttaaagacgt taatcagcag gaaagacatg accagcatca
    10921 gcagcagttg gtcgttgttc cgcagcccgg caagatgttc gcgcagggta aatttcccca
    10981 ttgtggagag cggcacccgt tcacgaaccc agaagaaaca gcacaggaac atcaccacgg
    11041 caatggcgca caataccccg acgcccagtt gatagccgcg tgcagcgttg ccctgaccga
    11101 agagagctac catccacggt aagcctaccg aaaccagaaa tcccgctacg ccgcagagaa
    11161 cgaatcgcca ggactggcag gagatcactt cattgtggcg ggtggtcatg gtgttgatcc
    11221 gcgcgcagta cggtacgttg atggcggtat aaccgactga aaggagcagg taagtaccga
    11281 acgcccaggc gatttttact cccatgctgg catctggcac ggtgaacgtc aggatgccga
    11341 taatgccgat gggaatggca atccatagct gccacggacg aaagcgcccc catcggcttt
    11401 gcgtccggtc ggcaatgacg cccatcaccg ggtcggagat ggcatcaaac acgcgcagtg
    11461 caataaacag cgtgccaacc agcgccggag ttaatccaaa aacgtcggtg tagaaaaagg
    11521 tgagaaaatt catgatcagg caagttatta cggtgccgcc tgcgtcaccc aggccgtagc
    11581 ctattttttc ccgtacagat aacgcatcgt caattttacc tttagcgata tcagcggtag
    11641 taatcggtgt agaggtcata gatattcctc gtgaataggg agcaattttt agttttattg
    11701 aaatgacctg attaattctg ccgtgaacgg gaatatcaac aaggcgagaa aagagtaaaa
    11761 aaaggtgacg attccgacct gaatttaaaa gtgtgaaatg gatcaggtga aattaatgtt
    11821 tttgattaat aatcaaaagg taatgtgttt aa
//
LOCUS       DAZKFZ010000135        11810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000135 DAZKFZ010000000
VERSION     DAZKFZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            139..432
                     /locus_tag="ACHOQW_003276"
                     /pseudo
     CDS             139..432
                     /locus_tag="ACHOQW_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(490..921)
                     /gene="tnpA"
                     /locus_tag="ACHOQW_003277"
     CDS             complement(490..921)
                     /gene="tnpA"
                     /locus_tag="ACHOQW_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFP9574307.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            929..2137
                     /locus_tag="ACHOQW_003278"
     CDS             929..2137
                     /locus_tag="ACHOQW_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9574308.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2181..2288
                     /locus_tag="ACHOQW_003279"
                     /pseudo
     CDS             2181..2288
                     /locus_tag="ACHOQW_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2272..2910)
                     /gene="ytfB"
                     /locus_tag="ACHOQW_003280"
     CDS             complement(2272..2910)
                     /gene="ytfB"
                     /locus_tag="ACHOQW_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP9574309.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3128..3748
                     /gene="fklB"
                     /locus_tag="ACHOQW_003281"
     CDS             3128..3748
                     /gene="fklB"
                     /locus_tag="ACHOQW_003281"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9574310.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4057..5469
                     /gene="cycA"
                     /locus_tag="ACHOQW_003282"
                     /gene_synonym="dagA"
     CDS             4057..5469
                     /gene="cycA"
                     /locus_tag="ACHOQW_003282"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP9574311.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5514..6176)
                     /gene="ytfE"
                     /locus_tag="ACHOQW_003283"
     CDS             complement(5514..6176)
                     /gene="ytfE"
                     /locus_tag="ACHOQW_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP9574312.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6284..7249)
                     /gene="ytfF"
                     /locus_tag="ACHOQW_003284"
     CDS             complement(6284..7249)
                     /gene="ytfF"
                     /locus_tag="ACHOQW_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9574313.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7357..8217)
                     /gene="qorB"
                     /locus_tag="ACHOQW_003285"
     CDS             complement(7357..8217)
                     /gene="qorB"
                     /locus_tag="ACHOQW_003285"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9574314.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8306..8686
                     /gene="ytfH"
                     /locus_tag="ACHOQW_003286"
     CDS             8306..8686
                     /gene="ytfH"
                     /locus_tag="ACHOQW_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9574315.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8804..10747)
                     /gene="cpdB"
                     /locus_tag="ACHOQW_003287"
     CDS             complement(8804..10747)
                     /gene="cpdB"
                     /locus_tag="ACHOQW_003287"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP9574316.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10937..11677
                     /gene="cysQ"
                     /locus_tag="ACHOQW_003288"
     CDS             10937..11677
                     /gene="cysQ"
                     /locus_tag="ACHOQW_003288"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP9574317.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIERNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2797 a         2949 c         3071 g         2993 t
ORIGIN      
        1 aatcgctcat tatggatata acgatgcacc cggggagaaa atgacttcca cgccttacac
       61 cgtacattct tgttgtgcgg tatgtaatta ttctacgctg tcggtatcgg tgtattcgtt
      121 aatcagcagg ttaaccagcc acaaaaagcc attgagaaaa ttattgattt tacatgggat
      181 tattatattg ctaacccctg gtttttaaaa attgtgcata gtgaaaatca aagtaaaggt
      241 gtgcattatg caaaatcaca gcgtctctta gaaatcaacc atgcgcactt gcagttaatg
      301 gaatccttat tggatgaagg gaagaaatac aatattttta agccagatat cgacccattg
      361 caggtgaata ttaatattgc tgcgcttggg ggatattatt tgatcaacca gcatacgctt
      421 ggcctggttt atccggtgcg ccgtaaaacc ccgtccttca gggcggggat atacggcgcg
      481 gttttccacc taaccaggtg tttgctgttg ttcaatgtat tgacggatgg cggatattgg
      541 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
      601 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
      661 gtttaccaga ctggatatcg ccagtttggg aggatagtta attaacaaat ggacgtgatc
      721 tggttcgcca tccatttcaa ccagttcagc ttcaaaatca gcacatacct ttgaaaagta
      781 agtgcgtagt ttttctgtcg cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa
      841 gaccaggtga acatgcatca ggaaaacaca atgccttcca cgccggatat cggtttcttt
      901 tttcacagac caaagtatca tttggcctgt gaaacgacta caggcattta aattccagtt
      961 aagacccggt ggtcaacagg agcgtgaaat gaggcgcttc gcaggcgcat gtcgtttcgt
     1021 tttcaatcgt gctctggcgc ttcagaatga gaatcatgag gccggtaata aatacatccc
     1081 ttacgggaaa atggcttcct ggctggttga gtggaaaaat gccactgaaa cgcaatggct
     1141 taaagatgcc ccgtcacagc cattgcaaca gtcactgaaa gaccttgagc gggcttacaa
     1201 aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggggac agaatgatgc
     1261 attccgctac ccgcagggcg ttaagctcga tcaggaaaac agccgtattt ttctgccgaa
     1321 acttggctgg atgcgctacc ggaatagccg tcaggtcacg ggtgttgtga aaaatgtcac
     1381 tgccagccag tcctgcggta agtggtacat cagtattcag actgaaaatg aagtatcaac
     1441 tccggttcac ccttcagcat taatggtcgg actggatgct ggcgtggcta aactcgccac
     1501 gctgtcagat ggcacagtct ttggacctgt aaacagtttt cagaaaaacc agaagacgct
     1561 ggcgagactt cagcgacagt taagccgcaa ggtcaaattc agcaacaact ggcagaagca
     1621 gaaacgcaaa atacagcgac tgcattcctg tatcgcaaat atccgcaggg actaccttca
     1681 caaagtcaca acgaccgtca gcaaaaacca cgcaatgata gtcattgagg atttgaaggt
     1741 cagcaacatg tcaaagtcag cagcgggtac ggtcagccag ccggggcgca atgtccgggc
     1801 aaaatcaggt ttaaaccgtt cgatactgga tcagggctgg tatgaaatgc gccgccagct
     1861 tgagtacaag cagctctggc gtggcggtca ggtgcttgct gttccgccag cgtacacaag
     1921 ccagcgttgc gcgtgctgtg gtcatacagc gaaagaaaat cgcctgtcac aaagtaaatt
     1981 cagatgccag gcatgtggat atacagcgaa cgctgatgta aatggcgctc gtaacatttt
     2041 agcggcgggg cacgccgttc ttgcctgtgg ggagatggtg cagtcaggcc gcccgttgaa
     2101 gcaggaaccc accgaaatga ttcaggcgac agcctgaacg tagcagggat ccacgtcctt
     2161 cagggcttgg aggatgtcaa cacatcagta tggtttcacc ccaggcactg gaagccagac
     2221 gtaaggtcat caaagagaca atccttagct ggcttttggt tgacccttca tctaccgcgc
     2281 acgaataaaa ctgccatccg gctggcgggt gaacaggacc tgttgattat tccccgtatc
     2341 aatggttaag cccgtcacca cgccgctggc gttctggcgg attttcacca tctgcccgtt
     2401 ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc gccattgcat agacatcggt
     2461 agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt ttaccgggtt cgacacggta
     2521 agagcgccat tgattatcaa ttccgctatc tggctggaat ggttgcgttt gggtagtttt
     2581 cggctgttct tctggctggc cttcctgtat aggttctgga gcgaccggag ccacctgatc
     2641 aggataattt tgtggggtga ctaattgcgc ccgcagctgt tcttctgtcg gcggttgtga
     2701 ttgcgactga atatctaact gcgcttcacg cgtcaccacc ggcgcgttgg gcgtatcatc
     2761 agatggaagc agaaatccga ccaccaatac aatggcggca ataatgatcc cccgacgatg
     2821 catcggcggc agcgggtcca tgaagcgaaa attgtccggt gcgtgccaga ctttctccag
     2881 ggttggtttt agttcaaagc gcccgggcat ggtttcctcc tgctccgcgt cttgttcctc
     2941 aatcatagcc tatgaataag ctaacgctat gatgtccgtg gtaaacccgc ctttattata
     3001 ttcatacggg atattgctat tgtttctttt tccctgggat ttgtcatcat tcccgcgaca
     3061 aagttttacc caaagaagtg tggctgatat gctgcccgct actttatacc ctaagaaagg
     3121 aaatacgatg accaccccaa cttttgacac catcgaagcg caagcaagct acggcattgg
     3181 tttgcaggta gggcaacaac tgagtgaatc tggcctggaa gggctgctgc cagaagcact
     3241 ggttgcaggt attgccgatg cgctggaagg caaacatccg gctgttccgg ttgatgtggt
     3301 gcatcgcgcg ctgcgtgaaa tccacgagcg cgccgatgcc gttcgtcgtc agcgtttcca
     3361 ggcgatggct gctgaaggtg tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa
     3421 tagcaccgaa tctggcctgc aattccgcgt gatcaaccag ggtgaaggcg caattccggc
     3481 acgtaccgac cgcgttcgtg ttcattacac cggtaaactg atcgacggca ccgtgtttga
     3541 cagctccgtt gctcgtggtg aacccgctga atttccggtt aatggcgtga tccctggctg
     3601 gattgaagca ctgactctga tgccggtagg ttctaaatgg gaactgacta tcccgcagga
     3661 actggcatat ggcgagcgcg gcgcaggcgc atccatccct ccgttcagca ccctggtgtt
     3721 tgaagtcgaa ctgctggaaa tcctctaagc agcgcattct gttcccctcg aaccagaggg
     3781 gagcaggcat tcagcaataa acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa
     3841 gaccccgtaa gcgtgtattt ttgtgagctg tttcgcgtta tcaccgtgat atgacactca
     3901 ctttaaacat aaaattaaca ttagatctaa atcttagtat tcatcccgcg tattgttacc
     3961 taatatcgat gagtcccgat acagattcgt cgtatcatag actgactaaa ggccgtagag
     4021 cctgaacaac acagacaggt acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt
     4081 gccgatgatc aggctccggc tgaacagtcg ctacggcgca atctcacaaa ccgacatatt
     4141 cagcttattg ccattggtgg tgccattggt acggggttgt ttatggggtc tggcaaaacg
     4201 attagccttg ccgggccgtc gatcattttc gtttatatga tcattggttt tatgctcttt
     4261 ttcgtgatgc gggcaatggg ggaattgctg ctttcgaatc tggaatacaa atcttttagt
     4321 gacttcgctt ccgatttact cgggccgtgg gcaggatatt tcaccggctg gacttactgg
     4381 ttctgctggg ttgtaaccgg tatggcagac gtggtggcga tcacggctta tgctcagttc
     4441 tggttccccg atctctccga ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg
     4501 cttaacctcg ccactgtgaa aatgttcggt gagatggagt tctggtttgc gatgatcaaa
     4561 atcgtcgcca ttgtgtcgct gattgtcgtc ggcctggtca tggtggcgat gcactttcag
     4621 tcaccgactg gtgtggaagc gtcattcgcg catttgtgga atgacggcgg ctggttcccg
     4681 aaaggtttaa gtggcttctt tgccggattc cagatagcgg ttttcgcttt cgtggggatt
     4741 gagctggtag gtacaacagc tgcggaaacc aaagatccag agaaatcact gccacgcgcg
     4801 attaactcca ttccgatccg tatcattatg ttctacgtct tcgcgctgat tgttattatg
     4861 tccgtgacgc cgtggagttc ggtagtcccg gagaaaagcc cgtttgttga actgtttgtg
     4921 ttggtaggtt tgcctgcggc tgccagcgtg atcaactttg tggtgctgac ctctgcggcg
     4981 tcttccgcta acagtggcgt cttctctacc agccgtatgc tgtttggtct ggcgcaggaa
     5041 ggtgtggcac cgaaagcgtt cgctaaactt tctaagcgcg cagtacccgc gaaagggttg
     5101 actttctcgt gtatctgtct gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg
     5161 attggcgcgt tcacgatgat tacaaccgtt tccgcgattc tgtttatgtt cgtctggacg
     5221 attatccttt gctcgtacct tgtgtatcgc aaacagcgtc ctcatctaca tgagaagtcg
     5281 atctacaaga tgccgctcgg caagctgatg tgctgggtat gtatggcgtt ctttgtgttc
     5341 gtggtcgtgt tgctgacact ggaagatgac actcgccagg cgctgctggt taccccgctg
     5401 tggtttatcg cgctggggct gggctggttg tttattggta agaagcgggc tgctgaactg
     5461 cggaaataac cgcattatca tgctggatgg cgcaatgcca tccagctttt agatcactca
     5521 cccgccagcg cccttgggaa cagtacattg ttttccaggc tgatgtgatc catcaggtca
     5581 tcaatcagtt cattaatgcc gttatacatc gctttccagg tggtgcaggc ttccggcggc
     5641 ggtgtgacgt tattggtatt gtgtttgatc acttccagca gttcacccgc ttcatcgtgc
     5701 tcgctttcca ttacgctgat tggccccatt gcctggctgc ccatgccttg tttgatcatc
     5761 gggaagagga tctgctcttc tttcatcatg tggctggaaa gctcttcatg cagcatggtc
     5821 aggtattttg tcagcccttt tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta
     5881 gtcgcttgca gaatcagctc cggcagttgc tcgcggtgac gagcgtggta gcgcacgatg
     5941 atatggtcga tgatttctgc cagcggggcg ctacgccagt ctttctcaat cggttgttca
     6001 gcaagctttg ccagctcagc ttcaatgacc tcaacatcca gttctttacg tgccgccgca
     6061 cgcgccagcg tttgcttacc gccgcagcag taatccatat catatttacg aaacagagcc
     6121 gaagcgcgag gaatagagag cgccagttca cctaaaggtt ggtcgcgata agccatagct
     6181 gatacctcat tcttaataag ataagatgta ttttaaatgc atctttaagg caaaagctat
     6241 aacccttacg tagcagaggc ttatttaact cactgccagc gtgtcaactt tctgagacag
     6301 attcagttaa aggtttttct ggttttgctc taaccgcgat gaccacgcca atcactaaca
     6361 gcgcgatacc gctcagcgtc attagcggcg gcatttgctg gcggagtaaa aaggtgtaca
     6421 gcaaacctgc cagcgtttcg aaaacaatca gcggcccgag aatcactgtc ggtaatcgct
     6481 ggctggcgac gttccagcag agtgcgccaa cccatgagca aagcacggct atcgcaatca
     6541 tcagactaat aaacaccagc ggacgggggc caaagggtag ggagaagtcc ggcgtttgcg
     6601 tattcagcca gtaacaagcg acgagatagc cgataagtga aaccggcagt gtgaccagcg
     6661 cctgcgctgt tgcccacatc atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt
     6721 tgcgcagggc ataccacgcc cagcagacca cggaaactaa cgccagcacg atgccagagg
     6781 tataacgtgc ccagtcaaaa ttggggagtc cgtggtttaa ctcagcaata ttcacacacg
     6841 ccaggccgat gccaatacaa atcagtgccg gggcgagttt tccccacgcg agtttgccgt
     6901 cgcgctggct ataaagcaga ttggcgaaga cgggaatcac caccggcagg gtgccgataa
     6961 tcatcgtgga aacaggcgca ccagtacgtt gaatggcact ggcaaggcag aagtaataga
     7021 tgaggttgcc catcatagtg agcatcaagg cggtaagcca gtcgcgacgc gccaactgac
     7081 gcagacgcac gcgtcccagc caggcaatgg gcagcgcaat taaccctaac gccagataac
     7141 gccccatcga ctgcaacatc gccgggtatt ccggcacgat caacggcccg acaaaaataa
     7201 gcccccacat caaccctgct aacagggcgt acagcacgcc gctaatcatt actggcatcc
     7261 attgatctgt cagaagaaag ttcaggaagc agacatgatt gctcccggca tcccgtcatt
     7321 atcagagagg atgcgggagg atgccacttt aattaactag ttattaacat taaaaagatg
     7381 gcttacgctt tcggctaacg ttgtcgttgg gtggccaatc aatttgctaa gtgttttgct
     7441 gtcatcaaac agaccgcctt tcgatgcgcc aacgtcagaa tccgccagca tatccgccag
     7501 tccgtcgggc agtccgacgc ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg
     7561 ataggtaacc tgtttgccgc tctgtttggt cagctctgcc gctaactgtg tcaacgtcca
     7621 ggcactatcg cccgccagtt cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg
     7681 tgccgcagct gccgcataat ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc
     7741 accgataaat acgccgtgtt ccagtgctgc cggggcgctg gcgaggtagt tttcggtgta
     7801 ccagccgttg cgcagtaggg tgtaaacgat gccagaatca gccagcattt tctccgtctc
     7861 gatgtgctca tcggcgaggc cgagcggaga tttatccgcg tgaagcaggc tggtataagc
     7921 gataaatttc acgccagccg tctttgcggc attaataaca ttacgatgct gcggggcacg
     7981 ttgacccact tcgctggaag agatcagcag tagtttttcc actccctgaa gtgcagatgt
     8041 cagtgcggct tcatcgccgt agtcagcctg acgcacggta atgccttgtg ctgtcagtgc
     8101 ctgggctttt gccggattac gaacgatagc cactatttgg ctggcaggaa ccgttttcat
     8161 caaggattca ataacatact gaccaagttg gccagtggca ccagtaatag cgatcatggg
     8221 aagtctccat cgttttgtct tgtgtttaaa tacgctaaca cctaaactta cttttagtaa
     8281 gtacgcacaa aaaggtaagt atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga
     8341 gggtaatctc tttgcggaac agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg
     8401 ttggggggtg ttgattctgg tggcgctacg cgaaggtact catcgcttta gcgacctgcg
     8461 gcgcaaaatc ggcggggtga gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca
     8521 ggatggtttt cttaaccgta tcgcgtatcc ggtggtgccg ccgcatgttg aatacagcct
     8581 cacgccgctg ggcgagcagg tgagcgaaaa ggttgctgca ctggcggact ggattgagtt
     8641 gaatttgccc gaggtgttgg cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg
     8701 gcctacggtg tatgacttgt agggcggata agatgcgcca gcatcgcatc cgccaataaa
     8761 tgccggatgc gattgatgat ttataggccg aaaaagaagt gttttactta ctcaaatcca
     8821 cctgataaat cgcaaacccg atatcatcgg tcgcgacttt attcatcgga tactgccctt
     8881 tctctttaat aaacgctgcg gctttatctg acggggaagt ttcgaaacgg atatccagtt
     8941 tcttatcggc agctatcggt gctaaacgcc agttgttatc tgccgccggg tgaatttccc
     9001 ccgcacgttt cgactcatca gcaatccacg ctgccagcac cgagcggttc tcatccggtg
     9061 aagcaaaagc gatatggctg tcgcccgtcc cggcaaattt gccgccgtaa gcgcgatagt
     9121 tattggtggc aacgaggaac atagcgttcg gatcaatcgg cttgccatta aaggtcaggt
     9181 tcttaatcct ttccgcattg gcattaatca tctggcactc gccgtcataa cgggcgggct
     9241 gggtaacatc aatctgataa ttcacaccat caataacatc aaagttataa gtgcggaaac
     9301 catcccagtt gatgagtgac tgtggtttcg tgctgttggg atcaatctgg ttaaactgtc
     9361 cggcggagca ctccagccac tctttcacct ctttaccgct ggctttcacc acaatcagcg
     9421 tattgggata gagataaaga tcggcggcat tacggaaggt caactggcct ttttccacct
     9481 ccacatagct tgccgggtca tttttgcgac caccgacttt aaacggtgcg gcagctgaaa
     9541 gcaccggcag ttttgccaga tccggatcgc cctgaatgta atgctcgaca tacgcttttt
     9601 gcgcgttgtt cactacttgc acggtcggat cgtcctgcac cagcgccaga tagctataca
     9661 tattgtcggc agatttaccg attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg
     9721 ctttgagtgt ttctaccagc ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt
     9781 cgtaaatcgg ccgtgcttcc gctttcgcct gcgtcacctg ccatttaccg ctgtcattac
     9841 tgagttgtaa gtcgaccacc ccaagatgat cgccccacat gcccggcatt accgccggaa
     9901 caccattcag cgtgcctttg gtgatatcag ccccttcgat atcagcaaaa tctttacccg
     9961 ggaaaacggc gtgagcatgg ccaaacataa tggcgttaac gcccggaatt tcactgaggt
    10021 aataaactga gttttccgcc attactttat acggatcggc agacagcccg gaatgcgcca
    10081 gaacgacaac gacatcggca cctttctcgc gcatttcagg ctcgtatttg cgcacggttt
    10141 cggtaatatc attcaccgtc acttttccgg ataaattagc tttatcccag cccatgattt
    10201 gcggcggcac gacgccaata tagccaatct tcagcgtctg tttttttccg tctttatcga
    10261 ccacttcggt gtctttaatt aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa
    10321 tgacgttggc atttacataa gggaatttcg ctcctgccag cgcatttttc aggtaatcca
    10381 gaccgtagtt aaactcgtgg ttgccaagcg ttccgacggt atagtccagc gtatttaatg
    10441 ccttatagac cgggtgaata tcacctgctt ttaatccttt cgccgacatg taatcggcca
    10501 gcggactccc ctgaatcaaa tcgccgttat cgaccagtac gctgtttttc acttcattgc
    10561 gggcattgtt aatcaggctt gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt
    10621 tgtaataatc gaaatccatc atgttgctat gcagatcagt ggtttccatg atacgcagat
    10681 cgaccgtcgc tgcattcaca ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact
    10741 taatcatcag ggacatcctt ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc
    10801 ttacttatag aacagtgaag aatgccacaa ttttacgctt tgaaaatgat gacactatca
    10861 cagttggcgc attcattaat gatagggtat aagtaaaaca ataagttaac accgctcaca
    10921 gagacgaggt ggagaaatgt tagatcaagt atgccagctt gcacggaatg caggcgatgc
    10981 cattatgcag gtctacgacg gggcgaaacc gatggacgtc gtcagcaaag cggacaattc
    11041 tccggtaacg gcagcggata ttgccgctca caccgttatc atggacggtt tacgtacgct
    11101 gacaccggat attccggtcc tttctgaaga agatcctccc ggttgggaag tccgtcagca
    11161 ctggcagcgt tactggctgg tagacccgct ggatggtacg aaagagttta ttgaacgtaa
    11221 tggcgaattc accgttaaca ttgcgctcat tgaccatggc aaaccgattt taggtgtggt
    11281 gtatgcgccg gtaatgaacg taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga
    11341 gtgcggcgtg cgcaagcaga ttcaggtccg cgatgcgcgc ccgccgctgg tggtgatcag
    11401 ccgctctcat gcggatgcgg agctgaaaga gtatctgcaa cagcttggcg aacatcagac
    11461 cacgtccatc ggctcttcgc tgaagttctg cctggtggcg gaaggacagg cgcagctgta
    11521 cccgcgtttc ggaccaacga atatttggga caccgccgct ggacatgctg tggctgcagc
    11581 tgccggagcg cacgttcacg actggcaggg taaaccgctg gattacactc cgcgcgagtc
    11641 gttcctgaat ccggggttca gagtgtctat ttactaaatt cagatggcag aaacagtgta
    11701 tttcctgatt ctgccatcct gatttctccc aacctaaaaa gttataaata aaaagagatt
    11761 gtatttaaag tgcaaaaatt caattgctaa taagttacat tttaataatg
//
LOCUS       DAZKFZ010000136        11640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000136 DAZKFZ010000000
VERSION     DAZKFZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            121..4104
                     /gene="ehaA"
                     /locus_tag="ACHOQW_003289"
     CDS             121..4104
                     /gene="ehaA"
                     /locus_tag="ACHOQW_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFP9574318.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4246..5334
                     /locus_tag="ACHOQW_003290"
     CDS             4246..5334
                     /locus_tag="ACHOQW_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9574319.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5376..6307)
                     /locus_tag="ACHOQW_003291"
                     /pseudo
     CDS             complement(5376..6307)
                     /locus_tag="ACHOQW_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6399..6906)
                     /locus_tag="ACHOQW_003292"
                     /pseudo
     CDS             complement(6399..6906)
                     /locus_tag="ACHOQW_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7164..7769
                     /locus_tag="ACHOQW_003293"
     CDS             7164..7769
                     /locus_tag="ACHOQW_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9574320.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7809..8672
                     /locus_tag="ACHOQW_003294"
     CDS             7809..8672
                     /locus_tag="ACHOQW_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9574321.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8662..10209
                     /gene="fdrA"
                     /locus_tag="ACHOQW_003295"
     CDS             8662..10209
                     /gene="fdrA"
                     /locus_tag="ACHOQW_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9574322.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10209..11627
                     /locus_tag="ACHOQW_003296"
     CDS             10209..11627
                     /locus_tag="ACHOQW_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9574323.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         3070 a         2715 c         3155 g         2700 t
ORIGIN      
        1 tgccaatgct atgcaaaatt tttgaatttt tatatgcgat gaacagtaac cggaatggca
       61 tttaatgcgt tgctgtttat gcaatcttgg ttttatttgg atgtattgct ggagattgtg
      121 atgaataaaa tatatcggct aaagtggaac aggtcccgta actgttggag cgtctgctcg
      181 gagctgggga gcagagtaaa aggaaaaaag tcccgggctg ttttaattag cgcgataagt
      241 ttatattcat ctctggtatt cgccgatgat gtcatcgtaa accaggataa aactattgat
      301 tttggcaaag agaaccagag catcgattac cgtattacgg tgacagacaa tgccaatctg
      361 gtaatcaatg cgacagatac ttcccgtccg cgtctgactc tcgcttctgg tggtgggttg
      421 gatattaccg gaggaaaggt aacaatcaat ggcccactta actttttgct gaaaggtacg
      481 gggttcctga atgtctccaa tgctggcagc gagttatatg ctgatgattt gtatgaatca
      541 aactcaggca tgagacacga tcgcggctat tttaatgtct ccaacggcgg caaaatccat
      601 gttaagggca ccagccgtct gacctatttg cagggaaatg tcagtggtga aggtagccag
      661 gtaaattccg aaaccttctt tatgggcgtt tacggcagtt acggtggtaa tcagtacctg
      721 tcagttaata acggcggtga agttaatgcc aggaagcaaa ttagcctggg ctattatgat
      781 caagtctccg atacaacact tgctgtttcg gaaggtggta aaatttctgc gcctactatt
      841 agtttaagca ccaactctga gttagcgtta ggggcacagg aaggaagcgc agcgaaggca
      901 gcagggatta ttgatgccga aaaaattgag tttgtgtggg caaagacatc cgagaagaaa
      961 atcaccttaa accacacgga taaagacgcg actatttccg cggatattgt cagtggcagc
     1021 gagggcctgg gctatatcaa tgcgctcaat ggcacgactt acttaaccgg tgataactct
     1081 gcctttagtg gtaaagtcaa aattgagcaa aatggcgctt tagggatcac ccaaaatata
     1141 ggtacagcag agatcaacaa ccgcgggaaa ttacacctga aggctgacga tagcatgacc
     1201 tttgccaata agatctctgg caacggtaca ataagtatcg acagtgggac ggtggagttg
     1261 accggcaata actatgcatt cagcggatat attgatgttg cttctggtgc tgtcgctgtt
     1321 atttctgaag acaagaatat cggtcgtgca gagctggatg tcgatggcaa attgcaaatt
     1381 aatgccaaca aagattgggt atttgataac gatcttgaag gtagaggcat tgttgaaata
     1441 aacatgggga atcacgaatt ctccttcgat gagtttgctt atacagactg gttccagggt
     1501 tcactggcgt tccagaacac gacatttaat ctggaaaaga atgctgagtt tctgcagaaa
     1561 ggcgggatca ctgcgggtca gggaagcctg gtaacagtgg gtaagggcgc tcactccatt
     1621 agcactttgg gattctccgg cggaaccgtt gattttggtg ccctgacagc aggtgcacag
     1681 atgacagaag ggacggtcaa cgttagtaaa acgctggatt tgcgcggcga gggtgtgatt
     1741 caggtttctg acagtgacgt tgtccgctca gtatctcgtg atattgactc tgcgttatcg
     1801 ctcactgaag tcgatgatgg taacagcacc attaagttgg ttgatgcgca aggtgcggaa
     1861 gttctgggcg atgcgggcaa tctgcaattg caggataaaa atgggcaaat cctctccagc
     1921 agcgcccaac gtgatattca gcagaatggg caaaaagcgg ccgtcggcac ttacgactat
     1981 cgtctgacga gtggggtaaa caatgacggt ctgtatattg gttacggcct gacccagctt
     2041 gatttacacg ctaccgacag cgatgctctg gtgctgagct ctaacggtaa aagcgagaat
     2101 gccgccgatc tcagcgcaaa gattaccggc agtggtgacc tggcattcag cagccagaag
     2161 ggtcagaccg tatcgctttc taacaaagac aacgactata ccggtgttac cgatctgcgc
     2221 agtgggacgc ttttgttgaa taacgataac gtgttgggta atacccatga actgcgtctg
     2281 gcggcagaga ctgaactgga catgaatggt cacagccaga ccgtgggcac gctcaatggc
     2341 agcgccgatt cactgctgag cttaaatggc ggcagtctga cggttaccaa cgggggcact
     2401 tcaaccggtt cgttaacggg gagcggagag ctgaatattc agggcggcac gctggacatc
     2461 gcgggcgata acagcaacct gacggcgaat gtgaacattg ctaattcggc taatgtcctg
     2521 gtaagtcatg cgcagggatt gggtagcgca aacgttgaga acaacggtac cctggcgttg
     2581 aataatagcg ctgaaaaaag agcggctgcg tctgtgaatt acgccctggg cggcaatctg
     2641 accaacaacg gtacgctgat gaccggaatg tcaggacagc aagctggcaa tgtgttagtg
     2701 gtgaagggga actaccacgg taataacggt caactagtaa tgaatacggt actgaatggc
     2761 gatgactcag taaccgataa attggttgtc gagggcgata ctagcggcac gactgccgtt
     2821 acggtgaata acgctggcgg tacaggtgcg aaaaccctta acggtatcga acttatccat
     2881 gtagacggta agtctgaggg cgaatttgtt caggctgggc gtatcgttgc gggggcgtat
     2941 gactacactc tcgcgcgtgg acaaggggca aatagtggta actggtatct gaccagcggc
     3001 agtgattctc ctgaactgca gccggagcca gacccgatgc cgaatccaga gccaaacccg
     3061 aatccagagc cgaaccctaa cccgacacct acgccgggtc cggatctgaa tgtggataat
     3121 gacctgcgac cggaggcggg tagctacatt gcgaaccttg cagcagcgaa taccatgttc
     3181 accacgcgtc tgcatgagcg tctgggtaat acgtactata ccgacatggt gacgggtgag
     3241 cagaaacaaa ccactatgtg gatgcgccat gaaggtggtc ataataaatg gcgtgatggc
     3301 agcggccagc tgaaaaccca aagcaatcgc tatgttctgc aactgggagg cgatgtcgcg
     3361 cagtggagcc aaaacggcag cgaccgctgg catgttgggg tcatggcggg atatggcaac
     3421 agcgacagca aaaccatttc ctcgcgaacc ggttatcgtg caaaagcgag tgtgaacgga
     3481 tatagcacag gcctctatgc cacctggtat gccgatgacg agtcgcgtaa tggcgcgtat
     3541 ctcgacagtt gggcgcagta cagctggttt gataacacag tgaaagggga tgacttacaa
     3601 agtgaatcct ataaatcaaa aggatttacc gcttcactgg aagctggata caaacacaaa
     3661 ttagctgaat ttaatggcag ccagggaacg cgtaatgaat ggtatgttca gccgcaagca
     3721 caggttacct ggatgggagt caaagccgat aagcaccgcg aaagcaacgg aaccctcgtt
     3781 catagcaacg gtgatggcaa tgttcaaacc cgacttggcg taaaaacctg gctgaagagc
     3841 caccataaaa tggatgacgg taaatcccgc gagttccagc cgtttgtaga agtgaactgg
     3901 ctacataaca gtaaggattt cagcaccagt atggatggcg tgtctgtcac tcaggatgga
     3961 gcccgaaata ttgctgagat aaaaaccggg gtggaaggac agctaaatgc caacctgaat
     4021 gtctggggga atgtgggcgt tcaggttgcc gataggggat ataatgacac ctctgcaatg
     4081 gttggcatta agtggcaatt ctgatctgaa taatgagtaa aaggagagag agccgaacgg
     4141 gagataattc ggctctctgt gtcattttct tgcagccagc tttatccgtc agagtgaaga
     4201 ctcgcagatt tagctggcgc acaagattta atcaaggtgt gttcaatggc ttcgcatgat
     4261 ttgagtgttt ttttggaaga gttcggggca acggtgaatt tgacgttgcc tggtatcgtg
     4321 tccgaaaaag aacggctttt actcaagctg ctaatggagg gaatgtcggt tacagaaatc
     4381 tcacagtaca gaaatcgcag tgcaaagaca atttcacatc aaaagaagca gctgtatgaa
     4441 aaactaggca tccagagtga tattaccttc tggcgggata tcttttttca atatcatccg
     4501 caagtcatta gtggcacggg aaataaaaat aatttttata tccctgataa ccgttgtcac
     4561 catatcgtca cgcctgaagc catcagtctg gcgttggaaa accatgaatt taaaccgtgg
     4621 atccaaccgg ttttctgcgc gcagactggg gtactgacgg gctgtgaagt gcttgtccgc
     4681 tgggaacatc cacaaacggg aatcatcccg ccggatcagt ttattcctct ggcggagtca
     4741 tccggtctta ttgtcataat gacccgccaa ctgatgaaac agactgcgga tattctgatg
     4801 ccggtaaaac atttgctgcc ggataatttc catattggta tcaacgtctc ggcgggttgt
     4861 tttttggccg cgggatttga aaaagaatgc ctgaacctgg tcaagaaatt aggaaacgat
     4921 aaaatcaaac tggttctcga gctaacggaa cgtaacccta ttccggtaac gccagaagcc
     4981 agagcgatat ttgacagcct tcatcagcat aacattacct ttgcgctgga tgactttggt
     5041 acgggttatg cgacctatcg ttatctgcag gcgtttccgg tcgattttat taagatcgat
     5101 aagtcatttg tgcaaatggc gggtgtcgac gaaatatccg gtcatattgt ggacaatatt
     5161 gtcgaactgg cgcgtaagcc tggtctgagt atcgtggcgg aaggggtaga aacccaggag
     5221 caggcggatt taatgatcgg taaaggcgtt cactttttgc agggctattt gtactctccg
     5281 ccagtaccgg gtaataaatt tatctctgaa tgggtaatga aagcaggtgg ttgatgtaaa
     5341 ccgctattca cagcgcatcg ggaggttggc agcgattaat tctcccgatg cagtaaatcc
     5401 tgatagatcc ccgttaatat gccatttggc gcaaattgtt ttttaatcca ctgcgtcacc
     5461 tgacccgtag cagaatgctg agtcgccagt aacatgcgtg aatcctggcg cgggttatgg
     5521 atttgtcggg taaccagcag ggattgcgcc atcgcctcac ggaccatgta atctggcaaa
     5581 aaaacaatcc cttcacctaa aatctggcac tgacatttgg tgttgaaatc tggcaccaga
     5641 attgactcct gcccgtgcag caaccagccc acttttttat taatcgtatg cgcggtgtcc
     5701 tccaccataa tattagggta gagacgcagt tggctttcgg cgatggttcc ggcacaaagg
     5761 ctaacgggtg atccggggcg atagcaaaag cccagcgaat cgcgccaatt tcagtgtaat
     5821 caataccgcc gccgtccagc agcgtgtcgg gtgcgccgat agcgatattt gcctgattat
     5881 taataatcgc atcccagacg ccgttataca cttcggtggt gacggtaatc tggcaggtag
     5941 gaaactgctt tttcagcacc tgtaataagc gtgcggtgtg tttgggcgta taaagcaact
     6001 gattaataca aatgcgcaca cgcgcttcta tgccctgcga aatagtatcg atgctgcgtt
     6061 tgatggcgtg aaaatcattc agcaggtcgg tagctttgcg gaaaaaataa cgcccggact
     6121 ccgttaactc aatgctgcgg gtactgcgag tgaacagcac cacatccagc cctgtttcca
     6181 ttcgcttgat ggtgtagcta atggcggaag tggttaaacc caactcttct gcggctttac
     6241 tgaaactgcc aaaacgcgca gcggtagtaa atgccaacag attctcttcg gtaaaaattg
     6301 agttcattaa accattcccg ccattatcta atatgaattt ctccaagcat aactgtagag
     6361 gtatcaatta gagatgggta atcatttttg aataactttt aatacccgtc gcgtttagtc
     6421 gtcgcttcgc ttaatttttg tccgccatat tctgaaatta tccccacgca gttacccgcc
     6481 agaaaaccca gtaacgtcac cgtccagttc atcccgctgg caaaaaacag cgtcatgccg
     6541 agaaagccac cgggaataaa tgacagtaac cagaaacgcc cttgccagac caccaccgcc
     6601 gagaagggta cggtagtaat tacgctcgcc cagaacgcgg acagtccact tacagaggcc
     6661 agccaaccgg cagccagcgc ggcgaaaaac gcccacacca tcccggaata attaaccgac
     6721 agacttctga taaaccccga tttaccgccg cctgcggcat aaaaactgca aaaggcgacg
     6781 aagccaatgg ttcctaataa ttcctaataa ttcccatccc tgagagagcc cgacatgtga
     6841 agacactaac tgccaaagcc ctgcacaaat accgacggtg acacccgttg ccgtgagtcc
     6901 gttcatcttg ttcccctctg gttagacatg gacgaaagct actcttacat gtgcagacta
     6961 tagagaaata aaacctgatt gagtgaaagg gtatcgggtc aaagaaacaa atattgcact
     7021 accgcacact gcgaaaagat tgttgaacat cattcaacaa aaaggcgaaa tacggcgaat
     7081 taaatttaag cgggttcttt tatcagatta tcgttgctat tctcaattca tcgaaaacag
     7141 ccacaaaaac caggggaatg tgaatgagta taaaaaatct acctgccgac tatttattgg
     7201 ctgcacaaca gggtgatatc gataaagtaa aaacctgcct tgcgctgggt gtcgatatta
     7261 atacctgcga tcgtcagggg aaaacggcaa ttacgctggc aagtttatat cagcaatatg
     7321 cttgcgttca ggcattaatt gatgccggag cggatattaa taagcaagat catacctgtt
     7381 taaatccttt tttaattagc tgtctgaacg gtgatttaac gctgctacga attattttac
     7441 cggctaaacc agaccttaat tgcgtcaccc gttttggcgg tgtcggcctg acgcctgcct
     7501 gtgaaaaagg ccatttaagt attgtaaaag agcttttggc gcatacggaa attaacgtta
     7561 accagaccaa ccatgttggc tggacgccac tactggaagc gattgtgctt aacgatggcg
     7621 ggattaaaca gcaggcgatt gtgcagttat tgctggaaca cggtgccagc ccgcatctga
     7681 ccgataaata tggcaaaacg ccactggaac tggcgcggga acggggcttt gaagagattg
     7741 cgcagttact gattgccgca ggtgcataaa ccgggaggct tgctatcaac acaccagaaa
     7801 aacggtgtgt gtgggcgcta actgcggatg cggattttct ggcgcagcga gggcaaggac
     7861 aggttgaaca ggtctttgcc agagcggtaa atatcgcact cccggctcgc cagcagttgc
     7921 tgacgctgct ttgtgaagag tacgacaatg cgccaaacag ttgtcggttg gcactcactc
     7981 actttgatga tctgttccgg catggtgata aggttcagtt tgacgatcaa ggtattacgg
     8041 ttggtcaaca tcttcatata gagatgagtc gttgtcggcg ttggctgtcc ccaaccttgc
     8101 aaatgaccgc tgtgaatttt cgccttatcg cctggcaaca gtggcacgac atcattcatc
     8161 agcacctggg ggaaaatgaa accctgttta attatcgcgg cgataatccg ttttatcagg
     8221 cgttaaataa agaattacat attaaacgac gggcagttat acaggcagta atcgataaac
     8281 aaaatatcgc ctcagcggtc gccagtatga tgggcttagg gattggcctt acgccatcag
     8341 ccgatgatta tttaacaggt ctggcactta ttttatttat ttccgggcat ccggcgggaa
     8401 aatacaaaga ggaattttat ctcggcttgc aacgcggcag aaataatacc acgttattaa
     8461 gtgccataac gctggaagcc gcattacaac aacgctgccg ggaaaatatt catcgtttta
     8521 ttcacaacat tatttatgac atccctggga acgcaactca ggcaatagaa aaaattaaac
     8581 atattggctc cagttccggc tgcgacatgc tgtatggcat ggccgatggt tgtgcgctga
     8641 gccaaaccta cggagggaat tatgtcagtt aaaatagtca ttaaaccgaa tacctatttt
     8701 gattctgtct cgctgatgtc tatctccacg cgtgcaaata aactcgacgg cgtcgagcag
     8761 gcatttgtgg cgatggcgac cgaaatgaac aaaggtgtgc tgaaaaattt aggactgctg
     8821 acgccggagc tggagcaggc gaaaaacggc gacctgatga ttgtcatcaa tggtaaatcg
     8881 ggggcggaca acgagcagtt gctggtggag attgaagaac tgttcaacac caaagcgcaa
     8941 agcggctcgc acgaggcgcg ttacgccact attgccagcg ccaaaaagca tatcccggaa
     9001 agtaacctgg cggtgatttc ggtcaacggt ctgtttgccg ctcgcgaagc gcgtcaggcg
     9061 ctgcaaaacg atctcaacgt gatgctgttt tccgataacg tctcagttga agatgaactg
     9121 gcgctcaagc aactggccca cgaaaaaggg ctgctgatga tggggccaga ctgcggcacg
     9181 gcgattatca acggtgcggc gctctgcttt ggtaacgccg tgcgtcgcgg caacatcggt
     9241 attgttggcg catccggcac cggcagtcag gagttgagcg tccgcattca tgaatttggc
     9301 ggcggcgttt cgcaactgat tggcaccggc gggcgcgacc tgagcgagaa aatcggcggc
     9361 ctgatgatgc tcgacgccat cgggatgctg gaaaacgatc cgcaaactga aatcattgcg
     9421 cttatctcca aaccgcctgc gcctgcggtg gcccgcaaag tgctggaacg tgcgcgcgcc
     9481 tgccgcaagc cggtggtcgt ttgcttcctc ggtcgtggcg aaacgccagt ggatgagcag
     9541 gggctacagt ttgcccgcgg caccaaagag gcagcgctaa aagcggtgat gctctccggc
     9601 gtgaaacaag aacatctcga cctgcatacc ctgaaccagc cgttgattac tgacgtgcgc
     9661 gctcgcctga agccgcagca gaaatacatt cgtggcctgt tctgcggtgg cacgctgtgc
     9721 gatgaaacga tgttcgcggt gatggaaaaa cacagcgatg tctacagcaa catccagccc
     9781 gatccggaat tccgcctgaa ggatatcaac cgcagcatca aacacacctt cctcgacttt
     9841 ggcgatgacg acttcaccaa cggcaagccg cacccgatga ttgaccctac caaccgcatc
     9901 agccgcttga tcgaagaggc gcgcgatcca gaagtggcgg tgatcgtgat ggattttgtg
     9961 ctcggatttg gatcgcatga agatccggtc ggttccacca tcgaggcgat caaagaagcg
    10021 aaagcgatcg ccgctgccga aggacgcgag ttgatcattc tcgcctatgt gctgggtacc
    10081 gatcttgata cgccatcgtt agaacaacaa agccagatgc tgcttgatgc cggcgtgatt
    10141 ctggcgagca gcagcaccaa taccggattg ctggcgcgtg aatttatctg caaaggggag
    10201 gaagcctgat gagccagtca ctgtttagcc aaccattgaa cgttattaac gtcggcatcg
    10261 ccatgtttag cgatgacctg aaaaagcagc atgtagaagt gactcaattc gactggacgc
    10321 cgccggggca gggcaatatg caggtggtgc aggcgctgga taacattgcc gattcgccgc
    10381 tggcagacaa aatcgccgcc gctaaccagc aggcgctgga gcgcattatc cagtcgcatc
    10441 cggtgctgat tggttttgat caggcgatta acgtggtgcc gggcatgacg ccgaaaacca
    10501 ttcttcacgc cgggccgccg atcacctggg aaaaaatgtg cggcgcgatg aaaggcgcgg
    10561 tcaccggagc gctggtgttc gaaggactgg cgaaagatct cgacgaggcg gctgaactgg
    10621 cggcttccgg ggagatcacc ttctcgccgt gtcacgagca cgactgcgtg ggatcgatgg
    10681 cgggtgtgac ctcggcctcg atgtttatgc acatcgtaaa aaacaaaacc tacggcaaca
    10741 tcgcttatac caacatgagc gagcagatgg cgaagatttt gcgtatgggc gctaacgacc
    10801 agagcgtgat tgaccgcctg aactggatgc gtgatgtgca gggaccaata ctgcgcgacg
    10861 cgatgaaaat tatcggcgaa atcgatctgc gcttaatgct ggcgcaggcg ctgcatatgg
    10921 gcgatgagtg ccataaccgc aataacgccg ggacgacgct gctgattcag gcgctgacgc
    10981 cggggattat ccaggcgggc tattccgtcg cgcaacagcg cgaagtgttt gagtttgttg
    11041 ccagtagcga ttacttctcc ggcccgacgt ggatggcgat gtgtaaagcg gcgatggatg
    11101 cggcgcacgg catcgaatac agcaccgtcg ttaccaccat ggcgcgtaac ggcgtcgagt
    11161 tcggcctgcg ggtgagcggc ctgccggggc agtggtttac cggcccagcg cagcaggtca
    11221 tcggcccgat gtttgctggt tataagccgg aagattcggg gctggatatc ggcgacagcg
    11281 ccatcacaga aacctacggt attggcgggt ttgccatggc gaccgcgcct gcaatcgtcg
    11341 cgctggtggg gggcacggtg gaagaggcta tcgatttctc ccgtcagatg cgcgaaatca
    11401 cccttggcga aaaccccaac gtcaccattc cgctgctcgg ttttatgggc gtgccgtcgg
    11461 cagtcgacat cacccgcgtg ggtagcagcg gcattctgcc ggtgatcaac accgccatcg
    11521 cccataaaga tgcgggcgtc ggcatgattg gcgcgggcat tgtgcatcca ccattcgcct
    11581 gcttcgagaa agccattctt ggctggtgcg aacgttacgg cgtttgacct acctgttcat
//
LOCUS       DAZKFZ010000137        11620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000137 DAZKFZ010000000
VERSION     DAZKFZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..624
                     /locus_tag="ACHOQW_003297"
     CDS             <1..624
                     /locus_tag="ACHOQW_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574324.1"
                     /translation="HFRYKFLLSGNAGEMILYFANVFFNPLALSFIIATIICITIKKR
                     SSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVKQGP
                     TLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQEGVQTM
                     SDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            747..1373
                     /locus_tag="ACHOQW_003298"
     CDS             747..1373
                     /locus_tag="ACHOQW_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574325.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1412..1657)
                     /gene="zapB"
                     /locus_tag="ACHOQW_003299"
     CDS             complement(1412..1657)
                     /gene="zapB"
                     /locus_tag="ACHOQW_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP9574326.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2083..2928
                     /gene="glpF"
                     /locus_tag="ACHOQW_003300"
     CDS             2083..2928
                     /gene="glpF"
                     /locus_tag="ACHOQW_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP9574327.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2951..4459
                     /gene="glpK"
                     /locus_tag="ACHOQW_003301"
     CDS             2951..4459
                     /gene="glpK"
                     /locus_tag="ACHOQW_003301"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9574328.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4689..5699
                     /gene="glpX"
                     /locus_tag="ACHOQW_003302"
     CDS             4689..5699
                     /gene="glpX"
                     /locus_tag="ACHOQW_003302"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9574329.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5796..6542
                     /gene="fpr"
                     /locus_tag="ACHOQW_003303"
     CDS             5796..6542
                     /gene="fpr"
                     /locus_tag="ACHOQW_003303"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP9574330.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6547..6975)
                     /gene="uspD"
                     /locus_tag="ACHOQW_003304"
     CDS             complement(6547..6975)
                     /gene="uspD"
                     /locus_tag="ACHOQW_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP9574331.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(7002..7301)
                     /locus_tag="ACHOQW_003305"
     CDS             complement(7002..7301)
                     /locus_tag="ACHOQW_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP9574332.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7513..7953)
                     /locus_tag="ACHOQW_003306"
     CDS             complement(7513..7953)
                     /locus_tag="ACHOQW_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9574333.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8054..8653
                     /locus_tag="ACHOQW_003307"
     CDS             8054..8653
                     /locus_tag="ACHOQW_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP9574334.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8761..9528
                     /gene="tpiA"
                     /locus_tag="ACHOQW_003308"
     CDS             8761..9528
                     /gene="tpiA"
                     /locus_tag="ACHOQW_003308"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9574335.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9583..10338)
                     /gene="cdh"
                     /locus_tag="ACHOQW_003309"
     CDS             complement(9583..10338)
                     /gene="cdh"
                     /locus_tag="ACHOQW_003309"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP9574336.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10445..11434)
                     /gene="sbp"
                     /locus_tag="ACHOQW_003310"
     CDS             complement(10445..11434)
                     /gene="sbp"
                     /locus_tag="ACHOQW_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP9574337.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11431..>11620)
                     /locus_tag="ACHOQW_003311"
     CDS             complement(11431..>11620)
                     /locus_tag="ACHOQW_003311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574338.1"
                     /translation="GVRAASGIDKAHFVSNMRRISSAFLTPNIYPQVIANLFLFFNVW
                     LTFWHALLITTQHKRVGR"
BASE COUNT         2950 a         2891 c         2850 g         2929 t
ORIGIN      
        1 catttccgct acaaatttct tttatctggc aacgcgggtg aaatgatttt atattttgct
       61 aacgtatttt ttaacccact tgccttatca tttattatcg ccacaatcat ctgtataacg
      121 ataaaaaaaa gaagcagccg cagctttatt cgcggcactt gttggttaat gggattattt
      181 cttatctata gcagctatac ggtgtgggaa cgacacagca tatgggatta tactttcccg
      241 gagccaggta tcaccgtcac ggttccgagc aaacagtggg ttgccaatat agtaaaacaa
      301 ggaccaaccc tggtgacccg ggatgcccat gctattttag ctattgttgc cttctcacaa
      361 aatgaactcg gtgtgcattc aatcgaggaa cttaccgcga ctcaaaacgg aactgcagaa
      421 atgcatgttt gtgatgttca gggatttgcc tgcgcttatc aggaaggagt gcaaacaatg
      481 agtgatggca aagtaagaca tgctattttc atgactctac tggataacac taatttgata
      541 caactggtcg cagctatcga cccagattat ttggacgaat atcaagaaga agtaatgaaa
      601 atgatgcttt ctgctcgtca atgatctgat gtgcatgtta ataataatgg attgatatga
      661 gatacgttat cttattaata ctatggataa taactgtttt tcttagtcat cttcagcaca
      721 gtaacttgct tactggagat ccagggatgg ttggcgagta tatcggttca gtacttcttg
      781 gaccgttatt acttcccgtg cttgtgtcag gtattctgtg cacattcact aaaaaaacgc
      841 gtaacttcgc cagttttact cgcgggtgct gttgggtatt gggagtcctg cttttatcca
      901 acgtcggcaa tacgtttcgt ctgttcaccc cgtggcacta tacgtttgag aaggcagcta
      961 tttccgtgac tgtcccaaac cgacactgga atacggtttc tattagtact gataaaacca
     1021 ttgatataag aagtgaagat aatagcgtct ttatttctgc tttccgcctg cccgcaggac
     1081 gtagcgccga tgactcgctg gaagagttaa agaagatgca gcgagacaat ctcaaggatc
     1141 aatacaatga agaaaccttc cagtttcatg attgtaatgc caaacatttc acctgtaagt
     1201 atcaggatgt gttgataaat tttgatggtc agcagaaaag aaccatcagt gtttatcttg
     1261 aagacacccc cagggctgtg gggatcattg cattaatgga gccagatacg gcagataaat
     1321 atcgccaaca ggcgatggag attatgttgt ctgctaaaaa tacagtgaag taaaaaataa
     1381 ggcgccttgc ggcgcctctg cgtggaagag attaaacctc ttccatgcga cccagcaggg
     1441 cctgcagacg ttcctgccag ccgttctgct gttctttcag atggttgttc tcacgctcca
     1501 gctcttcgcg ctgatgctgg gcattttgaa cttcctgcga caatgagttg tttttttctt
     1561 tcagctcttc gatttccatc tgcaacagag tgatggtatc aatcgcctgc tgtacttttg
     1621 cttccagttt ctcaaacact tctaatgaca ttgtcatacc tctcctgaat tgcaaggcgt
     1681 tgatggataa aaaatcctcg tcccgattac cggtgacgcc ttaataaata cgagcgcact
     1741 ttagttagct ccgattgtat gaagccgcgc catcgctgtc cagcggcacg ccttgcagat
     1801 tacggtttgc cacacttttc atccttctcc tggtgacata atccacacca atcgaaaatg
     1861 ttaataaatt tgttgcgcga atgttctaac aaacatgcat catgtacaat cagatggaat
     1921 aaatggcgcg ataacgctca ttttatgacg aagcacacac attttaagtt cgatatttct
     1981 cgtttttgct cgttaacgat aagtttacag catgcctaca agcatcgtgg aggtccgtga
     2041 ctttcacgca tacaacaaac attaactctt caggatccga ttatgagtca aacatcaacc
     2101 ttgaaaggcc agtgcattgc tgaattcctc ggtaccgggt tgttgatttt tttcggtgtg
     2161 ggttgcgttg cagcactaaa agtcgctggt gcgtcttttg gtcagtggga aatcagtgtc
     2221 atttggggac tgggggtggc aatggccatc tacctgaccg caggggtttc cggcgcgcat
     2281 cttaatcccg ctgttaccat tgcattgtgg ctgtttgcct gtttcgacaa gcgcaaagtt
     2341 attcctttta tcgtttcaca agttgccggc gctttctgtg ctgcggcttt agtttacggg
     2401 ctttactaca atttattttt cgacttcgag cagactcatc acattgttcg cggcagcgtt
     2461 gaaagtgttg atctggcagg cactttctct acttacccta atcctcatat caattttgtg
     2521 caggctttcg cagttgagat ggtgattacc gctattctga tggggctgat cctggcgtta
     2581 acggacgatg gcaacggtgt accacgcggc cctttggctc ccttgctgat tggtctactg
     2641 attgcggtca ttggcgcatc tatgggccca ttgacgggtt ttgccatgaa cccagcgcgt
     2701 gacttcggtc cgaaagtctt tgcctggctg gcgggctggg gcaatgtcgc ctttaccggc
     2761 ggcagagaca ttccttactt cctggtgccg ctttttggcc ctatcgttgg cgcgattgta
     2821 ggtgcatttg cctaccgcaa actgattggt cgccatttgc cttgcgatat ctgtgttgtg
     2881 gaagaaaagg agaccacaac tccttcagaa caaaaagctt cgctgtaata tgactacggg
     2941 acaattaaac atgactgaaa aaaaatatat cgttgcgctc gaccagggca ccaccagctc
     3001 ccgcgcggtc gtaatggatc acgatgccaa tatcattagc gtgtcgcagc gcgaatttga
     3061 gcaaatctac ccaaaaccag gctgggtaga acacgaccca atggaaatct gggccaccca
     3121 aagctccacg ctggtagaag tgctggcgaa agccgatatc agttccgatc aaattgcagc
     3181 tatcggtatt acgaaccagc gtgaaaccac tattgtctgg gaaaaagaaa ccggcaagcc
     3241 tatctataac gccattgtct ggcagtgccg tcgtaccgca gaaatctgcg agcatttaaa
     3301 acgtgacggt ttagaagatt atatccgcag caataccggt ctggtgattg acccgtactt
     3361 ttctggcacc aaagtgaagt ggatcctcga ccatgtggaa ggctctcgcg agcgtgcgcg
     3421 tcgtggtgaa ttgctgtttg gtacggttga tacgtggctt atctggaaaa tgactcaggg
     3481 ccgtgtccat gtgaccgatt acaccaacgc ctctcgtacc atgttgttca acatccatac
     3541 cctggactgg gacgacaaaa tgctggaagt gctggatatt ccgcgcgaga tgctgccaga
     3601 agtgcgtcgt tcttccgaag tgtacggtca gactaacatt ggcggcaaag gcggcacgcg
     3661 tattccaatc tccgggatcg ccggtgacca gcaggccgcg ctgtttggtc agttgtgcgt
     3721 gaaagaaggg atggcgaaga acacctatgg cactggctgc tttatgctga tgaacactgg
     3781 cgagaaagcg gtgaaatcag aaaacggcct actgaccacc atcgcctgcg gcccgactgg
     3841 cgaagtgaac tatgcgttgg aaggtgcggt gtttatggca ggcgcatcca ttcagtggct
     3901 gcgcgatgag atgaagttga ttaacgacgc ctacgattcc gaatatttcg ccaccaaagt
     3961 gcaaaacacc aatggtgtgt atgtggttcc ggcatttacc gggctgggtg cgccgtactg
     4021 ggacccgtat gcgcgcgggg cgattttcgg tctgactcgt ggggtgaacg ctaaccacat
     4081 tatacgcgcg acgctggagt ctattgctta tcagacgcgt gacgtgctgg aagcgatgca
     4141 ggccgactct ggtatccgtc tgcacgccct gcgcgtggat ggtggcgcag tagcaaacaa
     4201 tttcctgatg cagttccagt ctgatattct cggcacccgc gttgagcgcc cggaagttcg
     4261 cgaagtcacc gcattgggtg cggcctatct cgcaggcctg gcggttggct tctggcagaa
     4321 cctcgacgag ctgcaagaga aagcggtgat tgagcgcgag ttccgtccag gcatcgaaac
     4381 cactgagcgt aattaccgtt acgcaggctg gaaaaaagcg gtcaaacgcg cgatggcgtg
     4441 ggaagaacac gacgagtaat gtcaggcacc tgaatcgatt gcctgatgct gatcgcctga
     4501 tgcgacgctt acgcgtctta tcaggcctac gagccaatgc cattttgtgt aggcgggata
     4561 aggcgttcac gccgcatccg gcatcacgca gactctgaaa ccgtgcgggg gcaaccccgc
     4621 acacatcaat aatcccaccc ttcccctgtg ctacacttcg cgccattcct tactgcttag
     4681 agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa tctgcggcgc
     4741 tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc gcggcggtaa
     4801 acgccatgcg tattatgctc aaccaggtca acattgacgg caccatcgtc attggtgaag
     4861 gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact ggtcgcggcg
     4921 acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg atgggtcagg
     4981 ctaactcgct ggcggtgctg gcagtaggcg ataaaggctg tttcctcaac gcgccagata
     5041 tgtatatgga gaagctgatc gtcggaccgg gagccaaagg caccattgat ctgaacctgc
     5101 cgctggcgga taacctgcgc aatgtcgcgg cggcgctcgg caaaccgttg agcgaactga
     5161 cggtaacgat tctggctaaa ccacgccacg atgccgttat cgctgaaatg cagcaactcg
     5221 gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc acctgtatgc
     5281 cagacagcga agttgacgtg ctgtacggaa ttggtggcgc gccggaaggc gtggtttctg
     5341 cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg cgtcatgacg
     5401 tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca cgctgcaaag
     5461 cgatgggcat cgaatccggt aaagtattgc gtctgggcga tatggcgcgc agcgataacg
     5521 tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt agccgcaaag
     5581 gcaatatcgc gactaccgaa acgctgctga tccgcggcaa atcacgcacc attcgccgca
     5641 ttcagtctat ccactatctg gatcgcaaag acccggaaat gcaggtgcac atcctctgat
     5701 tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt tcggagaacg
     5761 aagataaggc aattcaatca aaacaggaga aaaacatggc tgattgggta acaggcaaag
     5821 tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac gcccctgtgc
     5881 atccgtttac cgccgggcaa tttaccaagc ttggccttga aatcgacggc gaacgcgtcc
     5941 aacgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt tacctggtca
     6001 ccgtccccga tggcaaatta agcccacgat tggcggcact gaaaccgggc gatgaagtgc
     6061 aggtggttag cgaagcagct ggcttctttg ttctggatga agtaccagat tgcgaaacgc
     6121 tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg caactaggta
     6181 aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat gccgccgact
     6241 taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa ctgcgcattc
     6301 agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata ccggcattaa
     6361 ttgaaagtgg tgaactggaa agcgcgattg gcctgccgat gaataaagaa accagccatg
     6421 tgatgctgtg cggcaatcca cagatggtgc gcgatacgca acagttgctg aaagagaccc
     6481 ggcagatgac gaaacattta cgtcgccgac cgggtcacat gacagcggag cattactggt
     6541 aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcaact tattgatcat
     6601 cccgcgatat gccggcatca aacggttgat aaatgagtgg tgatgaccac agacaaggag
     6661 gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc cgcgttcaat
     6721 acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct tgttatccga
     6781 cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac ccggatacaa
     6841 ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat gtctggcgag
     6901 ctccaaggct ttattcacca gtaaggcatc ttcttcattc ccggaaattg ccacgccaat
     6961 gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa cgaacgcagt
     7021 gataactcaa aaataatcat ctcggcctgg catgagaagg tgaaggccgc atcaagctca
     7081 acttgcccct ccagatcctt aatcttatag gccacgctgg ccccttcatc tgcagaggat
     7141 gcagcggcgg caagctcttt cagtttggtc ataaaggact ctgcttcttc gcgggtggca
     7201 aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc gatatcaatc
     7261 gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct ctgtatattc
     7321 gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac aaacaagtaa
     7381 tgatcttatg gattaccatt ttttttgata ttgcgtaagg tgaagagttc agaaggattt
     7441 tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata gcagtaagag
     7501 atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat cctgagtatc
     7561 tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga taagcatcat
     7621 caccagaatc aacgtcggga caaaacgccc taccgcccat tgccagacac ccggtaaaat
     7681 cgcccagtta cccgccagta gcatccacgc cacaatcatc agaaatgccc atgcgccgga
     7741 acgcccgcga tcatgcaagc gcttaacggt tactgccgct gttggccaga gcaagcacac
     7801 aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg agaaaagcac
     7861 cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc caatacgccc
     7921 tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt tgtcttttcc
     7981 aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc agtcataaca
     8041 aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt ctgcattaac
     8101 cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc cttatctgct
     8161 ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact ttaacagcca
     8221 gaatcccagc ctgccactga acgaatttcg tgccatcgac agcagtcccg acaaagccaa
     8281 tctcactcgt gctgccagta aaattaacga gaacttgtat gcttctacag cgctggagcg
     8341 cggtacctta aaaatcaaaa gcattcaaat gacatggcta cccatccagg ggccagagca
     8401 aaaagccgcg aaagcgaaag ctcaggaata catggcagcg gtgatccgca cactcacccc
     8461 attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctactaacgg cggggaaaaa
     8521 caaacgttat tacaccgaga cagaaggtgc actgcgctat gttgtcgcgg acaacggcga
     8581 aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat cgcttgaagg
     8641 tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac tgtttcacag
     8701 acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg gagaattaaa
     8761 atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca catggttcac
     8821 gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc ggttgcaatc
     8881 gcaccaccgg aaatgtacat cgatatggcg aagcgtgaag ctgaaggcag ccacatcatg
     8941 ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga aacctctgct
     9001 gctatgctga aagacatcgg cgcacagtac atcatcatcg gtcactctga acgtcgtact
     9061 taccacaaag aatctgacga actgatcgcg aaaaaattcg cggtgctgaa agagcagggc
     9121 ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg caaaactgaa
     9181 gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc ggcattcgaa
     9241 ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc tgcaactccg
     9301 gctcaggcac aggctgttca caaattcatt cgtgaccaca tcgctaaagt tgacgctaac
     9361 atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa cgctgcagaa
     9421 ctgtttgctc agccagacat cgacggcgcg ctggttggcg gtgcgtctct gaaagctgac
     9481 gctttcgcag taatcgttaa agctgcagaa gcggctaaac aggcttaagt ctgacaggtg
     9541 ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca aaatctcaca
     9601 ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat ttcgttgtgt
     9661 cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt agcttcccat
     9721 atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc gttgtaccag
     9781 ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac ctggcagtgg
     9841 caaccagcgg ctgctgatgt tcgccagatt gttatccagc tgttcgcgca catcaggacg
     9901 aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg agttgatcgc
     9961 caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt ttgctcataa aatcacgcgc
    10021 ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag gactttcagt
    10081 accgttaata cgatacgttg gcatcaacag atattgcagt gggccattaa gatcttttaa
    10141 aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt gattttgctg
    10201 ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact cttcaccggt
    10261 taatttccag taaccaatac cggcagccac aacggcgata actatcatca ccaaaaaaag
    10321 aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag ggtaacgcaa
    10381 aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa ttatcctggg
    10441 gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc tctttttgcg
    10501 ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt ggaaacgcat
    10561 tttcgtactt tttcgccacc tcagcgtcgc gcggacggta gtagtttttc gcggcaattt
    10621 cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct ttagtacctt
    10681 ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata gactcactcg
    10741 gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga gcttcgtttt
    10801 cccaggaaat cagtacatcg ccaattccgc gctcgacaaa agtgttagtg gagccgcgcg
    10861 cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc tgtgcttttg
    10921 cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc tgccaggtag ttccagcgcg
    10981 cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccagttta atcagatcgt
    11041 tccagtcatg gatctgcttc ggatttccct tacgtaccag gaaaacaatg gtggaagtgt
    11101 acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc ccgcgttccg
    11161 caatcgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca ataccgttga
    11221 ttaccgacgt tgcttgtttg cctgaaccac cgtgtgactg acgaatcacc acgttatcgc
    11281 cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac aattcgcgcg
    11341 ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg gttgccgcca
    11401 gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat gttgtgttgt
    11461 gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa gattggctat
    11521 aacttgcggg tatatgttgg gggttaaaaa ggcggaagaa atccgcctca tattgctgac
    11581 aaagtgcgct ttatcgatgc cggatgcggc gcgaacgcct
//
LOCUS       DAZKFZ010000138        11605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000138 DAZKFZ010000000
VERSION     DAZKFZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(146..322)
                     /locus_tag="ACHOQW_003312"
     CDS             complement(146..322)
                     /locus_tag="ACHOQW_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574339.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            625..1251
                     /locus_tag="ACHOQW_003313"
     CDS             625..1251
                     /locus_tag="ACHOQW_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9574340.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSK"
     gene            1602..1769
                     /locus_tag="ACHOQW_003314"
     CDS             1602..1769
                     /locus_tag="ACHOQW_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9574341.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(1849..3096)
                     /locus_tag="ACHOQW_003315"
     CDS             complement(1849..3096)
                     /locus_tag="ACHOQW_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP9574342.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3168..4082)
                     /locus_tag="ACHOQW_003316"
     CDS             complement(3168..4082)
                     /locus_tag="ACHOQW_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP9574343.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4298..5731
                     /gene="cscA"
                     /locus_tag="ACHOQW_003317"
     CDS             4298..5731
                     /gene="cscA"
                     /locus_tag="ACHOQW_003317"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9574344.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(5739..6734)
                     /locus_tag="ACHOQW_003318"
     CDS             complement(5739..6734)
                     /locus_tag="ACHOQW_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9574345.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            6977..7195
                     /locus_tag="ACHOQW_003319"
                     /pseudo
     CDS             6977..7195
                     /locus_tag="ACHOQW_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7213..8541
                     /gene="dsdA"
                     /locus_tag="ACHOQW_003320"
     CDS             7213..8541
                     /gene="dsdA"
                     /locus_tag="ACHOQW_003320"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP9574346.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(8649..10187)
                     /gene="emrY"
                     /locus_tag="ACHOQW_003321"
     CDS             complement(8649..10187)
                     /gene="emrY"
                     /locus_tag="ACHOQW_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP9574347.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10187..11350)
                     /gene="emrK"
                     /locus_tag="ACHOQW_003322"
     CDS             complement(10187..11350)
                     /gene="emrK"
                     /locus_tag="ACHOQW_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP9574348.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3340 a         2753 c         2610 g         2902 t
ORIGIN      
        1 caattaggtt taaaaattgt gatacttatg acagatttga gtgacatttt atcaatctac
       61 tgattttgat ggtgaaattt attagaaggt atgcgtagtg ctacgggaga aatgagaaac
      121 gcgcccagca atggcgcatc ctttcttaag caagttggct agtatctata gcatccatcc
      181 aatcaggagc aattcccaaa cgttccgcga actcttcatt ggtcacgtct tccattgaga
      241 gactagccag acaatcgtta atttcagaat agacggggga aagtaatagg tattcccgca
      301 actctttcag gtctttttcc atcttaacac tccttgttaa tagaccgtcg aacaatggta
      361 cagatttgta ccattccaag tactggcaag caaccccacc agcacaacaa attatctcat
      421 acggtaaaaa cggtgtcggg ctgtttcttt cggctagctc aatatatcac cttaaacacc
      481 agccgttatc attgtctcaa ttgggtatac cgtaatgaca aggtgataca aagtctcaat
      541 agggttgtat aggaacattt tgactacaca aactgcatta tgtatcattt gcacttatat
      601 tcacaaccct tttgagacaa tgcaatgaac gttagaattt actgccgagc atcaactgag
      661 gggcaacacg ctgatcgagc acttaccagc ctacgtgaat tttcaaaatc aaaaggctgg
      721 cagattgcag gagagtatat agaaaatgct agcggggcga agcttgagcg cgtagagctt
      781 atgcggctat tgtctgaagc ccagtccggc gatctgttgc tagtggaagc tatagatcgt
      841 ctgagtcgcc tagaacatag tgcatgggtc gaactgaaag atacgctcaa ccgaaaaggg
      901 ttaatcatcg tatcaatgga tttaccaacg agctggcaga tggtcgagat ggcaggcaat
      961 gatctaacca gcggcatcct gagagccgta aatgccatgt tgattgatat ccttgccaca
     1021 atggcacggc aggattacga aacacgccgt aaacgccaac agcaaggcat tgaacgagct
     1081 aaaagcgaag gcatatacat tgggagggcg aaaaatcagg aagcccgaga gatcgttaga
     1141 gagatgttgg agcaaggagt aaaacctgaa ctcatcatga aggcagcagg aatcagccga
     1201 gcgacttact accggataaa aaacgagcta ttgatcgtaa aatcaaaata aaatttgaaa
     1261 ggtcgtcagg cttgtttcat caggtaaaaa ccagcatttc tgatcacaac gcttgggggc
     1321 acctattggg gcactcacca aaatcgaaag agaaataaac tacaattcaa attgttagct
     1381 aatttttcga ttcctgcagg gacaccagat acccttcaaa ccaaatctac cttcatcccg
     1441 taaacgatgg gtttggcagc acacttgccc tatatctact catttttact gcaacaggtt
     1501 gaaatctcag cactgtcaga aagcgctgat gactaaacag cccagggccg ggcgatgtaa
     1561 ccatcacaca gaatcctgat agcgaaatac ggcgtgactc gatacttcac tccgcaatgc
     1621 attccttgat gaattcgcag gcccgtgata cacgggacag gtcgcagaat gacgacaatg
     1681 tcctggaaat cagcgaaccg tgtatccgga gtacatttga gcgactgtac cagaacatga
     1741 atgaggtgtt tggattaggc gattattagc agggctaagc attttagtat tattattttc
     1801 cggttgaagg atatggagat atcgacaaca accggaaaaa gtttacgtct atattgctga
     1861 aggtacaggc gtttccataa ctatttgctc gcgtttttta ctcaggaaga aaatgccaaa
     1921 tagcaacatc aggcagacaa tacccgaaat tgcgaagaaa actgtctggt agcctgcgtg
     1981 gtcaaagagt atcccagtcg gcgttgaaag cagcacaatc ccaagcgaac tggcaatttg
     2041 aaaaccaatc agaaagatcg tcgacgacag gcgcttatca aagtttgcca cgctgtattt
     2101 gaagacggat atgacacaaa gtggaacctc aatggcatgt aacagcttca ctaatgaaat
     2161 aatccagggg ttaacgaaca gcgcgcagga aaggatacgc aacgccataa tcacaacacc
     2221 gataagtaat gcattttttg gccctacccg attcacaaag aacggaataa tcgccatgca
     2281 cagcgcttcg agtaccacct ggaatgagtt gagataacca tacaggcgcg ttcctacatc
     2341 gtgtgattcg aataaacctg cataaaagac aggaaaaagt tgttgatcaa aaatgttata
     2401 gaaagaccac gtcccaacaa taaatatgac gaaaacccag aagtttcgat ccttgaaaac
     2461 tgcgataaaa tcctcttttt ttacccctcc cgcatccgcc gctacgcact ggtgatcctt
     2521 atctttaaaa cacatgttga tcatcataaa tacagcgcca aatagcgaga ccaaccagaa
     2581 gttgatatgg ggactgatac taaaaaatat gccggcaaag aacgcgccaa tagcatagcc
     2641 aaaagatccc caggcgcgcg ctgttccata ttcgaaatga aaatttcgcg ccattttttc
     2701 ggtgaagctg tcaagcaaac cgcatcccgc cagatacccc aggccaaaaa agagcgcccc
     2761 cagaattaga cctacagaaa aattgctttg cagtaacggt tcataaacgt aaatcataaa
     2821 cggtccggtc aagaccagga tgaaactcat acaccagatg agcggtttct tcagaccgag
     2881 tttatcctga acgatgccgt agaacatcat aaatagaatg ctggtaaact ggttgaccga
     2941 ataaagtgta cctaattccg tccctgtcaa ccctagatgt cctttcagcc aaatagcgta
     3001 taacgaccac cacagcgacc aggaaataaa aaagagaaat gagtaactgg atgcaaaacg
     3061 atagtacgca tttctgaatg gaatattcag tgccataatt acctgcctgt cgttaaaaaa
     3121 ttcacgtcct atttagagat aagagcgact tcgccgttta cttctcacta ttccagttct
     3181 tgtcgacatg gcagcgctgt cattgcccct ttcgccgtta ctgcaagcgc tccgcaacgt
     3241 tgagcgagat cgataattcg tcgcatttct ctctcatctg tagataatcc cgtagaggac
     3301 agacctgtga gtaacccggc aacgaacgca tctcccgccc ccgtgctatc gacacaatcc
     3361 acagacattc cagcaaaatg gtgaacttgt cctcgataac agaccaccac cccttctgca
     3421 cctttagtca ccaacagcat ggcgatctca tactcttttg ccagggcgca tatatcccga
     3481 tcgttctgtg tttttccact gataagtcgc cattcttctt ccgagagctt gacgacatcc
     3541 gccagttgta gcgcctgccg caaacacaag cggagcaaat gctcgtcttg ccatagatct
     3601 tcacgaatat tgggatcgaa gctgacaaaa cctccggcat gccggatctc cgtcatcgca
     3661 gtaaatgcgc tggtacgcga aggctcggca gacaacgcaa ttgaacagag atgtaaccat
     3721 tcgccatgtc gccagcaggg caagtctgtc gtctctaaaa aaagatcggc actggggcgg
     3781 accataaacg taaatgaacg ttccccttga tcgttcagat cgacaagcac cgtggatgtc
     3841 cggtgccatt catcttgctt cagatacgtg atatcgactc cctcagttag cagcgttctt
     3901 tgcattaatg caccaaaagg atcatccccc acccgaccta taaacccact tgttccgcct
     3961 aatctggcga ttcccaccgc aacgttagct ggcgcgccgc caggacaagg cagtagccgc
     4021 ccgtctgatt ctggcaagag atctacgacc gcatccccta aaacccatac tttggctgac
     4081 atttttttcc cttaaattca tctgacttac gcatagtgat aaacctcttt ttcgcaaaat
     4141 cgtcatggat ttactaaaac atgcatattc gatcacaaaa tgtcatagtt aacgttaaca
     4201 tttgtgatat tcatcgcatt tatgaaagta agggacttta tttttataaa agttaacgtt
     4261 aacaattcac caaatttgct taaccaggat gataaaaatg acgcaatctc gattgcatgc
     4321 ggcgcaaaac gcactagcaa aacttcacga gcgccgaggt aacactttct atccccattt
     4381 tcacctcgcg cctcctgccg ggtggatgaa cgatccaaac ggcctgatct ggtttaacga
     4441 tcgttatcac gcgttttatc aacatcaccc aatgagtgaa cactgggggc caatgcactg
     4501 gggacatgcc accagcgacg atatgatcca ctggcagcat gagcctattg cgctagcgcc
     4561 aggagacgag aatgacaaag atgggtgttt ttcaggtagt gctgtcgatg acaatggtgt
     4621 cctctcactt atctacaccg gacacgtctg gctcgatggt gcaggtaatg acgatgcaat
     4681 tcgcgaagta caatgtctgg ctaccagtcg ggatggtatt catttcgaga aacagggtgt
     4741 gatcctcact ccaccagaag gaatcatgca cttccgcgat cctaaagtgt ggcgtgaagc
     4801 cgacacatgg tggatggtag tcggggcgaa agacccaggc aacacggggc agatcctgct
     4861 ttatcgcggc agttcattac gtgaatggac tttcgatcgc gtactggccc acgctgatgc
     4921 gggtgaaagc tatatgtggg aatgtccgga ctttttcagc cttggcgatc agcattatct
     4981 gatgttttcc ccgcagggaa tgaatgccga gggatacagt taccgaaatc gctttcaaag
     5041 tggcgtaata cccggaatgt ggtcgccagg acgacttttt gcacaatccg ggcattttac
     5101 tgaacttgat aacgggcatg acttttatgc accacaaagc tttgtagcga aggatggtcg
     5161 gcgtattgtt atcggctgga tggatatgtg ggaatcgcca atgccctcaa aacgtgaagg
     5221 ctgggcaggc tgcatgacgc tggcgcgcga gctatcagag agcaatggca aacttctaca
     5281 acgcccggta cacgaagctg agtcgttacg ccagcagcat caatctatct ctccccgcac
     5341 aatcagcaat aaatatgttt tgcaggaaaa cgcgcaagca gttgagattc agttgcagtg
     5401 ggcgctgaag aacagtgatg ccgaacatta cggattacag ctcggcactg gaatgcggct
     5461 gtatattgat aaccaatctg agcgacttgt tttgtggcgg tattacccac acgagaattt
     5521 agacggctac cgtagtattc ccctcccgca gcgtgacacg ctcgccctaa ggatatttat
     5581 cgatacatca tccgtggaag tatttattaa cgacggggaa gcggtgatga gtagtcgaat
     5641 ctatccgcag ccagaagaac gggaactgtc gctttatgcc tcccacggag tggctgtggt
     5701 gcaacatgga gcactctggc aactgggtta acataatatc aggtggaaca acggatcaac
     5761 agcgggcaag ggatccgcgt cacacttccc ccttcacgac cttcaataat atgcaatgca
     5821 gcttcccgcc cgataatgtc atgtggaagc tgaattgtgg tcagcggcgg taaaaacaga
     5881 tgcccgacgc caaccagatt atcaaagccc attacggcga catcctgcgg gatacgtacc
     5941 cccttagcca ggagaacctg ataagccaca aaggctgcgc gatcgttacc acatatcaga
     6001 acatcaaaat ctggtttgcc cgatttgaag tggtcattga gtaaacttgc gagatcggtg
     6061 tagtgatcat cacctgttgc catgtgaaat tgtttcacct cagccagatc tcgtccagca
     6121 tcacgccagg cctgctcaaa tccctgccga cgataccctg ttgccaacgc actttccggt
     6181 agccagaagc ataacggttg acgatagccc gccgcgagca aatgctgtgt tgattcatat
     6241 tgtgcagtgt aatcatcagg gatataactg ggtaacgctg ggtcatccgc cacacagttc
     6301 gccaatacaa tattttcacc atacagagac tcaggtagcg tgatatgtcg cagccccatt
     6361 gtagtataga taatgccatc cggacggtgg gcaagcagct gacgtgccgc gcgggcagcg
     6421 tcatcttcag aaaaaatatt gattaaaaaa ctattccagc cgaactcgct ggcggtttgc
     6481 tcaatggcaa gcagaatatc aacagagaaa ggagtggtag cagtgtcctg cgccagcacg
     6541 gcgagagtcg acggcttacg tccttgagcg cgcatcttac gggcggaaag atcaggaaca
     6601 taattcaggg tctggattgc ctgcaatacg cggtcacgcg ttgcaggacg cacagattct
     6661 gcattatgca tcacccggga gactgtcatc atcgacactc ccgccaggcg tgcgacatcc
     6721 tttaatgaag ccatacccaa gccgtttgcc gtaaaacggg cactgtagca gaaacagacg
     6781 tcactggcga gatccaacga aattctcgac gccctatcac ccgacacggc aatacaataa
     6841 aaaataacaa taattgccgg ataattgtac ccaattccgc ctctgttctc gccaacgagt
     6901 gaccatatag gcaagtaaga aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     6961 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     7021 tgctatcggt tcaggcgcga ttggctgcac gatcgttacg gactcgcttt tctggctggt
     7081 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     7141 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     7201 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     7261 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     7321 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     7381 tcccgttttg caccctatct ggcaaaagca tttcctgaaa ctgctgccac tggggggatt
     7441 attgaatcag aactggttgc cattccggct atgcaaaaac ggctggaaaa ggaatatcaa
     7501 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     7561 ataaaagcac gcggcgggat ttatgaagtc ctggcacatg cagaaaaact ggctctggaa
     7621 gcggggttgc tgacgcttga agatgactac agcaaactgc tttctccgga gtttaaacag
     7681 ttctttagcc agtacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     7741 attatgagcg cccgcattgg ctttaaggtg acagtgcata tgtctgctga tgcccgggca
     7801 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     7861 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     7921 gatgacgaga attcccgcac gttgttcctt gggtattccg tcgcaggcca gcgtcttaaa
     7981 gcgcaatttg ccgagcaagg tcgtattgtc gatgctgata accctctgtt tgtctatctg
     8041 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg gacttaagct ggcgtttggc
     8101 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     8161 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     8221 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     8281 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     8341 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     8401 gtgtgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     8461 gccactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatggagcaa
     8521 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     8581 agcgatgctg ccgatggcgc agacttaaga tccccagtct tacccgctat aaccccctgc
     8641 ttttaccatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     8701 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     8761 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accatagtat
     8821 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggtttctgtc
     8881 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     8941 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     9001 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     9061 ccctgaaaaa actgcggcaa aatgatacct gtaaaatcaa tcgttggcat aaatgtcaca
     9121 gaacgccagt aatagcaaac cgcatacatc aaaaaactga atgtcaccaa cacccgcatg
     9181 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     9241 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     9301 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     9361 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     9421 tcccaaatga ctaaagagat cagaaaaata actgatacta ctgttaatat tattattgta
     9481 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     9541 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     9601 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     9661 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     9721 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt
     9781 ggataattcc ttaataataa actctgtgac agtggaatta acggccctgc cattaacccc
     9841 tgaacgactc taaaaaatat cagcacatca agattgatcg ataaactaca cattaaggaa
     9901 gacagcgaaa aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc
     9961 agcctgccgg taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag
    10021 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
    10081 atagtggaat ccaacatttg cataaacgtc gctaatgaca atgcaatagt gacgcaccat
    10141 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
    10201 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
    10261 tactggtata agccggcatg gacgtcacgg ttgaagccag ctcaggcatc tcagcaatgt
    10321 cttcgttctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
    10381 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
    10441 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
    10501 ctgtcacccg gccatgaaac acaacattct caccataaag atcgctgata atattgaccg
    10561 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttagcatta acccacattt
    10621 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
    10681 cacttctctg ggcaatatag ccggtaaccg gactcttaat atccgtacgt ttaagcgcca
    10741 accaggcttc tttggttgca tccgcagctt caacgacttg tggctgacgg tttaatggtg
    10801 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc aatgccgctt
    10861 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
    10921 tcgctaaagg cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg
    10981 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
    11041 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
    11101 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaacg acagtgacac
    11161 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
    11221 taatcatgtc ttctaattcc attgaccaat aggcataagc acctgaaaac gcaataaata
    11281 aaactatcgc caataaagaa aagtattttc ttctgttaga atgtttttta tttgaattaa
    11341 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
    11401 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatccctgg aacgttggag
    11461 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
    11521 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
    11581 catcggtata atccctgatt ttatt
//
LOCUS       DAZKFZ010000139        11547 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000139 DAZKFZ010000000
VERSION     DAZKFZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(99..1466)
                     /gene="rhlE"
                     /locus_tag="ACHOQW_003323"
     CDS             complement(99..1466)
                     /gene="rhlE"
                     /locus_tag="ACHOQW_003323"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP9574349.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            1695..2366
                     /gene="cecR"
                     /locus_tag="ACHOQW_003324"
     CDS             1695..2366
                     /gene="cecR"
                     /locus_tag="ACHOQW_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP9574350.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     YRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2369..3364
                     /gene="hlyD"
                     /locus_tag="ACHOQW_003325"
     CDS             2369..3364
                     /gene="hlyD"
                     /locus_tag="ACHOQW_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP9574351.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3357..5093
                     /gene="ybhF"
                     /locus_tag="ACHOQW_003326"
     CDS             3357..5093
                     /gene="ybhF"
                     /locus_tag="ACHOQW_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9574352.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5086..6219
                     /gene="ybhS"
                     /locus_tag="ACHOQW_003327"
     CDS             5086..6219
                     /gene="ybhS"
                     /locus_tag="ACHOQW_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9574353.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6230..7336
                     /gene="ybhR"
                     /locus_tag="ACHOQW_003328"
     CDS             6230..7336
                     /gene="ybhR"
                     /locus_tag="ACHOQW_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9574354.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7298..7708)
                     /gene="ybhQ"
                     /locus_tag="ACHOQW_003329"
     CDS             complement(7298..7708)
                     /gene="ybhQ"
                     /locus_tag="ACHOQW_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP9574355.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7841..8602
                     /gene="ybhP"
                     /locus_tag="ACHOQW_003330"
     CDS             7841..8602
                     /gene="ybhP"
                     /locus_tag="ACHOQW_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9574356.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8599..9840
                     /gene="clsB"
                     /locus_tag="ACHOQW_003331"
     CDS             8599..9840
                     /gene="clsB"
                     /locus_tag="ACHOQW_003331"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP9574357.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9840..10796
                     /gene="ybhN"
                     /locus_tag="ACHOQW_003332"
     CDS             9840..10796
                     /gene="ybhN"
                     /locus_tag="ACHOQW_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP9574358.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10832..11545)
                     /gene="ybhM"
                     /locus_tag="ACHOQW_003333"
     CDS             complement(10832..11545)
                     /gene="ybhM"
                     /locus_tag="ACHOQW_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9574359.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         2667 a         3074 c         3122 g         2684 t
ORIGIN      
        1 tttattaaaa caataaaatc agacatgtca tgcatgtctg accgtgttac ctgaataatg
       61 ggcaaaagcc tgtgatactc ttttgccctt acatggtatt actgcgcagc ggcaggttta
      121 cgcggacggc gacggcgttg ttgttcgcct gccggtttgg catcgccaag acgacgagat
      181 ggtttttctg caggcttcgc gctagctgat ttcgcaccac cttctccacg gcgtggttgc
      241 tgttgaccgc gaccaccacc accttgccca cgaccgccgc cgccacgttg ctggcgaccg
      301 ttctggatcg gttcggcttt gattgacggg tccggctcat aacccggaat cgcaatgcgc
      361 gggatctctt ttttcagcag tttttcgata tcacgcagca gtttgtgttc atcaacacac
      421 accagcgaca atgcttcgcc agttgccgcc gcacggccag tacgcccgat acggtggaca
      481 taatcttccg gtacgtttgg cagttcatag ttgacgacgt gcggcaactc ttcgatatcc
      541 agaccgcgcg cagcgatgtc agttgccacc agtacacgaa tatcgcccga tttaaaatcg
      601 gccagcgcac gggtacgcgc accttgcgat ttattaccgt ggatcgccgc actacggatg
      661 ccatctttat taagctgttc agcgagatgg ttagcgccat gtttggtacg ggtaaacacc
      721 agcacctgct gccagttccc tttcccaatc atgtgcgaca gcaattcgcg cttgcgtttc
      781 ttatcaacaa agtgaacatg ctgggtcacc tgatcagacg cggtattgcg gcgtgccact
      841 tctatttcca gcgggttgtg caacagtttt tccgccaggg ctttaatatc gtcagagaag
      901 gtcgcggaga acaacaggtt ctggcgcttc gcaggtagtt ttgttaacac gcgacggata
      961 tcgtggataa agcccatgtc gagcatgcgg tcagcttcat cgaggacgag gatttcaacc
     1021 tgatccagct tcactgcatt ctgatgttcc aggtccagca aacgtcccgg cgttgccacc
     1081 agcacatcaa cgccgccgcg caacttcatc atttgtgggt taatgctgac accgccaaac
     1141 accaccagcg aacgaatgtt caggtatttg ctgtaatcac ggacgttttc gccaatctgc
     1201 gccgccagtt cacgggtcgg ggtaagaatg agcgcacgta ccggacgacg ccctttggcg
     1261 tgcggctggc gagtgatcag gtgttgcaac agcggcagcg taaagcccgc tgttttgccg
     1321 gtgccggtct gggcgctagc catcaggtcg cggccttcca gcaccgcagg gatcgcctgc
     1381 tgctgaatag gggtgggttc acggtaaccc tgctcggcaa cggcgcgcag gatatcaggg
     1441 cttaaaccca aagaatcgaa agacataact actccgaacc gccccgaccg ttaccggtgt
     1501 agttttcagg gagatactga aaaggaaaat gacaaaaacc acaatgtcat gagggagcgg
     1561 agtgtagcag tttttgtgac gcagcgcata aattatccct gtgactggcg tagctaaaat
     1621 tcttagccat actggacaac tcccatatgg agtcataatc ttaatcaatc atttgattaa
     1681 gaatggactg tgcgatgaat aatcctgcca tgacaatcaa gggtgaacag gcgaaaaaac
     1741 agctgattgc tgccgcactg gcgcagtttg gtgaatatgg aatgaacgcc accacccgcg
     1801 agatagccgc ccaggccggg cagaatatcg ctgccatcac ctactacttc ggttcgaaag
     1861 aagatttgta cctcgcctgc gcccagtgga ttgccgattt tattggcgag cagttccgtc
     1921 cgcatgccga ggaagccgaa cgcttgttcg cccaaccaca gcctgatcgg gctgccatcc
     1981 gtgaactgat ccttcgcgcc tacaggaaca tgattaagct gctcacccag gatgacaccg
     2041 tcaacctcag caagtttatc tcccgtgagc agctctctcc cacggcagcc taccacctgg
     2101 tgcacgaaca ggtgattagt ccgctacaca gccacctgac acgcctgatt gccgcctgga
     2161 ccggctgcga cgccaatgac acccgcatga tccttcatac ccatgcgttg attggtgaga
     2221 ttctggcgtt ccgtcttggc aaagaaacaa ttctgttacg taccggctgg accgcgttcg
     2281 atgaagaaaa gaccgaactg atcaaccaga cggtgacctg tcatatcgac ctgattttgc
     2341 aaggattatc gcaaaggagt ttgtagtgat gaaaaaacct gtcgtgatcg gactggcggt
     2401 agtggtactt gccgccgtgg ttgccggagg ctactggtgg tatcaaagcc gccaggataa
     2461 cggcctgacg ctgtatggca acgtggatat tcgtacggtg aatcttagtt tccgtgttgg
     2521 ggggcgcgtt gaatcgctgg cggtggacga aggtgatgct atcaaagcgg gccaggtgct
     2581 gggcgaactg gatcacaagc cgtatgagat tgccctgatg caggcgaaag cgggcgtctc
     2641 ggtggcacag gcgcagtatg acctgatgct tgccgggtat cgcgatgaag aaatcgctca
     2701 ggccgccgca gcggtgaaac aggcgcaagc cgcctatgac tatgcgcaga acttctataa
     2761 ccgccagcaa ggactgtgga aaagccgcac catttcggca aatgacctgg aaaatgcccg
     2821 ctcctcgcgc gaccaggcac aggcaacgct gaaatcagca caggataaat tgcgtcagta
     2881 ccgttccggt aaccgtgaac aggacatcgc tcaggcgaaa gccagcctcg aacaggcgca
     2941 ggcgcaactg gcgcaggcgg agctgaattt acaggactca acgttgatag caccgtctga
     3001 tggcacgctg ttaacgcgcg cggtggagcc aggcacggtt ctcaatgaag gtggcacggt
     3061 gtttaccgtt tcactaacgc gtccggtgtg ggtgcgtgct tatgttgatg aacggaatct
     3121 tgaccaggcc cagccggggc gcaaagtgct gctttatacc gatggtcgcc cggacaagcc
     3181 ctatcacggg cagattggtt tcgtttcgcc gactgctgaa tttaccccga aaaccgtcga
     3241 aacaccggat ctgcgtaccg acctcgtcta tcgcctgcgt attgtggtga ccgacgccga
     3301 tgatgcgtta cgccagggaa tgccagtgac ggtacaattc ggtgacgagg caggacatga
     3361 atgatgccgt tatcacgctg aacggcctgg aaaaacgctt tccgggcatg gacaagcccg
     3421 ccgtcgcgcc gctcgattgc accattcatg ccggttatgt gacggggctg gtagggccag
     3481 acggtgcggg taaaaccacg ctgatgcgga tgctggcggg attactgaaa cccgacagcg
     3541 gcaatgccac ggtgattggc tttgatccga tcaaaaacga cggcgcgctg cacgccgtgc
     3601 tcggctatat gccgcagaaa ttcggcctgt atgaagatct gacggtgatg gagaacctca
     3661 atctgtacgc ggatttgcgc agcgtcaccg gcgaggcacg gaagcagact tttgctcgcc
     3721 tgctggagtt tacgtctctt ggcccgttta ccggacgcct ggcgggcaag ctctccggtg
     3781 ggatgaaaca aaaactcggc ctggcctgta ccctggtggg cgaaccgaaa gtgttgctgc
     3841 tcgatgaacc cggcgtcggc gttgacccta tctcacggcg cgaactgtgg cagatggtgc
     3901 atgagctggc gggcgaaggg atgttaatcc tctggagtac ctcgtatctc gacgaagccg
     3961 agcagtgccg tgacgtgtta ctgatgaacg aaggcgagtt gctgtatcag ggagaaccaa
     4021 aagccctgac acaaaccatg gccggacgca gctttctgat gaccagtcca cacgagggca
     4081 accgcaaact gttgcaacgc gccttgaaac tgccgcaggt cagcgacggc atgattcagg
     4141 ggaaatcggt acgtctgatc ctcaaaaaag aggccacacc agacgatatt cgccatgccg
     4201 acgggatgcc ggaaatcaac atcaacgaaa ctacgccgcg ttttgaagat gcgtttattg
     4261 atttgctggg cggtgccgga acctcagaat cgccgctggg tgcaatattg catacggtag
     4321 aaggcacacc cggcgagacg gtgatcgaag cgaaagaact gaccaagaaa tttggtgatt
     4381 ttgccgccac cgatcacgtc aactttgccg ttaaacgcgg ggagattttt ggtttgctgg
     4441 ggccaaacgg cgcgggcaaa tcgaccacct ttaagatgat gtgcggtttg ctggtgccga
     4501 cttccggcca ggcgctggtg ctggggatgg atctgaaaga gagttccggt aaagcgcgcc
     4561 agcatctcgg ctatatggcg caaaaatttt cgctctacgg caacctgacg gtcgaacaga
     4621 atttacgctt tttctctggt gtgtatggct tacgcggtcg ggcgcagaac gaaaaaatct
     4681 cccgtatgag cgaggcattt ggcctgaaaa gtattgcctc acacgccacc gatgaactgc
     4741 cattaggttt taaacagcgg ctggcgctgg cctgttcgct gatgcatgaa ccggatattc
     4801 tgtttctcga cgaaccgact tccggcgttg accccctcac ccgccgtgaa ttctggctgc
     4861 acatcaacag catggtagag aaaggcgtca cggtgatggt caccacccac tttatggatg
     4921 aagcggaata ttgcgaccgc atcggcctgg tgtaccgcgg gaaattaatc gccagcggca
     4981 cgccggacga tttgaaagca cagtcggcta acgatgagca acccgatccc accatggagc
     5041 aagcctttat tcagttgatc cacgactggg ataaggagca tagcaatgag taacccgatc
     5101 ctttcctggc gtcgcgtgcg ggcgctgtgc gtcaaagaga cgcggcagat cgttcgcgat
     5161 ccgagtagct ggctgattgc ggtagtgatc ccgctgttgc tgctgtttat ttttggttat
     5221 ggcattaacc tcgactccag caagctgcgg gtcgggattt tactggaaca gcgtagcgaa
     5281 gcagcgctgg atttcaccca caccatgacc ggttcgccct acatcgacgc caccatcagc
     5341 gataaccgtc aggaactgat cgccaaaatg caggcgggga aaattcgcgg tctggtggtt
     5401 attcccgtgg atttcgccga acagatggag cgcgccaacg ctaccgcacc gattcaggtg
     5461 atcaccgacg gcagtgagcc gaataccgct aactttgtgc agggatatgt cgaagggatc
     5521 tggcagatct ggcaaatgca gcgggcggag gataacgggc agacctttga gccgcttatt
     5581 gatgtacaaa cccgctactg gtttaacccg gcggcgatta gccagcactt tattatcccc
     5641 ggtgcggtga ccatcatcat gacggtcatc ggcgcgattc tcacctcgct ggtggtggcg
     5701 cgagaatggg aacgcggcac tatggaggct ctgctctcta cggagattac ccgcacggaa
     5761 ctgctgctgt gtaagctgat cccttattac tttctcggga tgctggcgat gttgctgtgt
     5821 atgctggtgt cagtgtttat tctcggcgtg ccgtatcgcg gttcgctgct gattctgttt
     5881 tttatctcca gcctgttttt actcagtacc ctggggatgg ggctgctgat ttccacgatt
     5941 acccgcaacc agttcaatgc cgctcaggtc gccctgaacg ccgcgttcct gccgtcgatt
     6001 atgctttccg gctttatttt tcagatcgac agtatgcccg cggtgatccg cgcggtgacg
     6061 tacattattc ccgcacgtta tttcgtcagc accctgcaaa gcctgttcct cgccgggaat
     6121 attccagtgg tgctggtggt gaacgtactg tttttgatcg cttcggcggt gatgtttatc
     6181 ggcctgacgt ggctgaaaac caaacgtcgg ctggattagg gagaagagca tgtttcatcg
     6241 cttatggacg ttaatccgca aagagttgca gtcgttgctg cgcgaaccac aaacccgcgc
     6301 gattctgatt ttaccggtgc tgattcaggt gattctgttc ccgttcgccg ccacgctgga
     6361 agtgaccaac gccaccatcg ccatctacga tgaagataac ggcgagcatt cggtggagct
     6421 gacccaacgt tttgcccgcg ccagcgcctt tacacatgtg ctgctgctga aaagcccaca
     6481 ggagatccgc ccgaccatcg acacacaaaa ggcgttacta ctggtgcgtt tcccggctga
     6541 cttctcgcgc aaactggata ccttccagac cgcgccgttg cagttgatcc tcgacgggcg
     6601 taactccaac agtgcgcaaa ttgccgccaa ctacctgcaa cagatcgtca aaaattatca
     6661 gcaggagctg ctggaaggaa aaccgaaacc taacaacagc gagctggtgg tacgcaactg
     6721 gtataacccg aatctcgact acaaatggtt tgtggtgccg tcgctgatcg ccatgatcac
     6781 cactattggc gtaatgatcg tcacttcact ttccgtcgcc cgcgaacgtg aacaaggtac
     6841 gctcgatcag ctactggttt cgccgctcac cacctggcag atcttcatcg gcaaagccgt
     6901 accggcgtta attgtcgcca ccttccaggc caccattgtg ctggcgattg gtatctgggc
     6961 gtatcaaatc cccttcgccg gatcgctggc gctgttctac tttacgatgg tgatttatgg
     7021 tttatcgctg gtgggattcg gtctgttgat ttcatcactc tgttcaacac aacagcaggc
     7081 gtttatcggc gtgtttgtct ttatgatgcc cgccattctc ctttccggtt acgtttcgcc
     7141 ggtggaaaac atgccagtat ggctgcaaaa cctgacgtgg attaatccta ttcgccactt
     7201 tacggacatt accaagcaga tttatttgaa ggatgcgagt ctggatattg tgtggaatag
     7261 tttgtggccg ctactggtga taacggccac gacagggtca gcggcgtacg cgatgtttag
     7321 acgtaaggtg atgtaacttc ttatctttcg ccagcaaaga cactaccgcc gggccggcaa
     7381 ggattgccag ccctgcaatt gccagcaaaa agttgttttc cagtacgcgg gacaacagcc
     7441 acagcacaat cagcgctgcg ccaaaatacc aggtcgtggt cagctcttcc agcacgtctg
     7501 ccacctcacg ccagcgttgc tcggggtggc gctgaaacac cagcatcatc actaccgtga
     7561 cacaatacag tgcgcataat cccacgccga cgtgaaccag agtcttactc atccagccca
     7621 ccaccagcag cgccactacc agtaaatgca acataatctg ccagcaactt agacccgttg
     7681 cgacacgaac acgttgttgc cacttcatgg cttctctcct gaagaattta tctctactta
     7741 ctcagagtac cgaactttac ggaaaattcc gtaagaccgc acctttttgt gacgccgact
     7801 acactatttc aatgagataa gaggcaaaga caggaaaatt atgcccgatc aaacacaaca
     7861 attttcgttc aaggtgctca ccatcaatat tcacaaaggc tttaccgcgt ttaaccgacg
     7921 cttcattttg ccggaacttc gcgatgccgt gcgtaccgtc agcgccgata ttgtttgcct
     7981 gcaggaagtg atgggcgcgc acgaagttca tccgctgcat gtggaaaact ggcccgatac
     8041 ctcgcactac gagtttctcg ccgacactat gtggagcgat tttgcctacg gtcgcaatgc
     8101 cgtatacccg gaagggcatc acggcaacgc cgtactgtcg cgttatccca ttgaacatta
     8161 tgagaatcgc gatgtttcgg tcgatggtgc ggaaaagcgc ggcgtgctct actgccgcat
     8221 tgtgccgccg atgaccggaa aagcgattca tgtgatgtgc gtacatctgg gcctgcgtga
     8281 ggcgcaccgt caggcgcagc ttgcgatgct cgccgaatgg gtgaatgagc taccggacgg
     8341 cgaaccggta ttggtggcgg gtgatttcaa tgactggcgg caaaaagcta atcatccgtt
     8401 aaaagtgcag gccggactgg atgagatttt tacccgcgcc cacggacgcc cggcgcgcac
     8461 gtttccggtg caatttcctc tactacgact ggacaggatc tacgtcaaaa atgccagcgc
     8521 cagcgcgcca accgcgttgc cgctgcggac atggcgacac ctttctgatc atgccccttt
     8581 aagtgcggag attcatttat gaaatgtagc tggcgcgaag gcaataagat ccagttgctg
     8641 gaaaacggcg agcaatatta tcccgcggtg tttaaggcga ttggcgaggc acaagaacgc
     8701 atcattcttg aaacgtttat ctggtttgag gatgacgtcg gcaaacaact gcatgcggca
     8761 ctactggcag cagcgcaacg cggggttaaa gcggaagtct tgctggatgg ctacggttcg
     8821 ccggatctca gcgatgagtt tgtcaatgaa ctgacggcag ctggcgtagt gttccgctac
     8881 tacgatcccc gccctcgcct ttttggtatg cgcaccaatg tgtttcgccg gatgcatcgc
     8941 aaaattgtgg taatcgacgc gcgtatcgcc tttattggcg ggctgaatta ctccgccgag
     9001 catatgtcca gctacggtcc agaggctaaa caggattacg cggtacgcct tgaagggccg
     9061 attgtcgaag atattctcca gtttgagctg gaaaacctgc ctggacagag cgcggcacga
     9121 cgctggtggc gacgtcatca caaagcggaa gagaaccgcc agccgggaga agcgcaggta
     9181 ttgctggtct ggcgcgataa cgaagaacat cgcgatgata ttgaacgtca ctatctgaaa
     9241 atgctcactc aggcgcggcg agaagtcatt atcgccaacg cctacttctt ccccggctat
     9301 cgatttttac acgccttgcg taaagcggca cggcgcgggg tgcggatcaa actgatcatt
     9361 cagggcgaac cggatatgcc gattgtcaga gtcggtgcgc gcttgctgta taactatctg
     9421 gttaaaggcg gcgttcaggt gtttgagtac cgccgccgcc cgctccacgg caaagtggca
     9481 ttgatggacg atcactgggc gacagtaggg tccagtaatc tcgatccgct cagtttgtca
     9541 ctgaatctcg aagcaaatgt catcatccac gatcgtcatt ttaaccagac gctgcgcgat
     9601 aatctgaacg gcattattgc cgcagattgt cagcaggtcg atgagaccat gctgccgaaa
     9661 cgcacctggt ggaacctaac caaaagcgtg ctggcgttcc actttttacg ccacttcccg
     9721 gcgctggttg gctggcttcc ggcacacacg ccacgtctgg cgcaggttga tcctcccgca
     9781 caaccgacaa tggaaacgca ggatcgggta gaaactgaaa acacgggggt aaaaccctga
     9841 tgagtaaatc acacccgcgc tggcgcttag caaagaagat cctcacctgg ctgtttttta
     9901 tcgcggtgat tgtgttgctg gtggtctacg ccaaaaaagt ggactgggaa gaggtctgga
     9961 aggtcatccg cgactacaat cgcgttgcgc tgcttagtgc ggtcgggctg gtggtcgtca
    10021 gctatctgat ttacggctgc tatgacctgc tcgcccgctt ttactgcggt cacaaactgg
    10081 cgaagcgcca ggtgatgctg gtgtcgttta tctgctacgc cttcaacctg acgctcagta
    10141 cctgggtcgg cggcattggt atgcgctatc gtttgtactc ccgtctggga ttgccgggca
    10201 gcactattac gcggattttc tcgctcagta ttaccaccaa ctggctgggc tatattttac
    10261 tggcagggat tatctttacc gcaggcgtgg tggagttgcc ggaccactgg tatgtcgatc
    10321 aaactacgct gcgtattctc ggcattggct tactgatgat tatcgcggtt tatttgtggt
    10381 tttgcgcttt cgcgaagcac cgccatatga ccatcaaagg gcaaaagctg gtgctgcctt
    10441 cgtggaaatt cgccctcgcc cagatgctga tttccagcgt taactggatg gtcatggggg
    10501 cgattatctg gctgttactt ggtcaaagcg tgaactattt ctttgtactg ggcgtgttgc
    10561 tggttagcag tattgccggc gtcatcgtgc atgttccggc ggggattggt gtgctggaag
    10621 cggtgtttat cgcgctactg gctggggagc atacatccaa aggcacaatt atcgccgccc
    10681 tactcgctta ccgtgtgctg tattacttta tcccgctgct gctggcgctg atctgttacc
    10741 tgttgctgga aagccaggcg aagaagctac gggcgaaaaa tgaagcggcg atgtgaagtt
    10801 tagttaagtt ctttagtatg tgcatttacg actaatgaaa aaaacgcgta tgcctttgcc
    10861 agacaagcgt tatagctatc gcggcaagta tcccaaagaa gccgataaaa tagtaataga
    10921 gtgttaatgc acctgtaaaa gcgcaacgaa ccggactcca caactcttca ctatggcatt
    10981 tccccgccaa ttcaaggagc ttatttcgtc catgcaagat aatgcctgac cataacacaa
    11041 tcattaagca acttattacc caaatgggcc gttcactcat taatgcggca ttcacgatga
    11101 ttaccagggc caaccctgtc aacgtcatca taatcataaa acggtgagaa ccaggatcaa
    11161 tattaaataa taaaccggcc agcatactga ttaaaaacat cgcgcccgca gtgctgaaca
    11221 cgacgataat ggcatataca ggaactaaac ttgcaacgac aatcgccacc aaagagagta
    11281 aaaacagata tattacgcct gcgatggcgg catttatctt gttgatatcc ttaattaagc
    11341 catacataca aagtaataat aaccctatgg cgacatacat tggtggtgaa tcatatttgg
    11401 ttgccgtaat ttcgagatac ttagcccccc aggccagccc tgcggttgcc accaaaccta
    11461 aagcggtaat gagccatata ccgtttaata tcctggtctc gtgctgaaaa tctaatttat
    11521 tgctgttttg caagtatgat tccattc
//
LOCUS       DAZKFZ010000140        11515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000140 DAZKFZ010000000
VERSION     DAZKFZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            206..976
                     /gene="yjjP"
                     /locus_tag="ACHOQW_003334"
     CDS             206..976
                     /gene="yjjP"
                     /locus_tag="ACHOQW_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP9574360.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            967..1440
                     /gene="yjjB"
                     /locus_tag="ACHOQW_003335"
     CDS             967..1440
                     /gene="yjjB"
                     /locus_tag="ACHOQW_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP9574361.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1547..2086
                     /gene="dnaT"
                     /locus_tag="ACHOQW_003336"
     CDS             1547..2086
                     /gene="dnaT"
                     /locus_tag="ACHOQW_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP9574362.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2089..2826
                     /gene="dnaC"
                     /locus_tag="ACHOQW_003337"
     CDS             2089..2826
                     /gene="dnaC"
                     /locus_tag="ACHOQW_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP9574363.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2875..3369
                     /gene="yjjA"
                     /locus_tag="ACHOQW_003338"
     CDS             2875..3369
                     /gene="yjjA"
                     /locus_tag="ACHOQW_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP9574364.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3623..5914
                     /gene="opgB"
                     /locus_tag="ACHOQW_003339"
     CDS             3623..5914
                     /gene="opgB"
                     /locus_tag="ACHOQW_003339"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP9574365.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6051..7073)
                     /gene="lgoD"
                     /locus_tag="ACHOQW_003340"
     CDS             complement(6051..7073)
                     /gene="lgoD"
                     /locus_tag="ACHOQW_003340"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP9574366.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7212..8126
                     /gene="lgoR"
                     /locus_tag="ACHOQW_003341"
     CDS             7212..8126
                     /gene="lgoR"
                     /locus_tag="ACHOQW_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9574367.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8341..9702
                     /gene="lgoT"
                     /locus_tag="ACHOQW_003342"
     CDS             8341..9702
                     /gene="lgoT"
                     /locus_tag="ACHOQW_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9574368.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9751..11415)
                     /gene="tsr"
                     /locus_tag="ACHOQW_003343"
     CDS             complement(9751..11415)
                     /gene="tsr"
                     /locus_tag="ACHOQW_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP9574369.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
BASE COUNT         2789 a         3045 c         2804 g         2877 t
ORIGIN      
        1 taaattcatt atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc
       61 tttttatcac cgagcttgtc ccgataacca ggattaatca gcgcaatttt gatcttaacc
      121 taccgcaaaa caaccttgct tcatgataca aatggattct cattatctga acaatacgca
      181 gcacgtctat gacaaagggc gagttatgca aactgagcaa cagcgagccg taacacggct
      241 ttgtatccag tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga
      301 gctttcctca cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc
      361 gaacgccata gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa
      421 tcacgatcgc ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc
      481 ggagcatcat ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcagccatt
      541 acgttaccca agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa
      601 actcaataac ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat
      661 gtatatccgc cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac
      721 cgctttcgcc gccaccacca tttccggatt gcttttgcaa caccccactt tcagcaatac
      781 ccccaccatt gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa
      841 tgccgtcgcc gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc
      901 cagtctgctg acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct
      961 acgcggatgg gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc
     1021 attcctgcgg tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt
     1081 gcgctgcttg gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat
     1141 attgagtggt caacctttat ggcctccatg ctggtcggca ccattggtat tcaatggtcg
     1201 cgctggtatc tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg
     1261 ggcatatcgg cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc
     1321 gaaccgttga tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg
     1381 ttatccatcg gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa
     1441 aattgcctcg cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata
     1501 aaagttatct cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt
     1561 tttgaccccg gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc
     1621 aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt
     1681 cacgcctgca cgcctggctg aactgctggc gctggaagaa aagctggcgc gtccgggaag
     1741 cgatgtcgat ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc
     1801 catggggaaa ttcgccatgt atccggactg gcaacccgat gccgacttta tccgcctggc
     1861 ggctctatgg ggcgtggcgc taagagagcc ggtaaccacc gaagaactgg cctcattcat
     1921 tgcctactgg caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc
     1981 gcgcagcctg caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac
     2041 ggtcagtgaa cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt
     2101 ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa
     2161 acgggtgaag agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc
     2221 gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgccca
     2281 ctgcatcaga actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg
     2341 ttaagcaaag cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt
     2401 tctggtaagc caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg
     2461 ctacgcggta aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat
     2521 accttcagga atagcggtac cagtgaagaa caactgctta acgatctgag caacgttgat
     2581 ctgctggtga tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc
     2641 aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt
     2701 aatatggaag agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt
     2761 aacagtttgt gggtgatctt caactgggat agctaccgta gccgtgtaac aggtaaagag
     2821 tattaatccc cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg
     2881 aaaactgtta aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg
     2941 gtgcatgctg cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa
     3001 aacagcacga cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc
     3061 ggaaaccagg ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc
     3121 gcgaagcaaa agctggcttc ggtaaccgat gtcgaaaaca tcaagaacca ggtgctggaa
     3181 aagctgggcc tgaacagtga agagcaaaaa gaagacacca actatctgga cggcattcag
     3241 ggtttgctga aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacaactccg
     3301 ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc
     3361 atttcctgaa ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct
     3421 gcccttttcc ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa
     3481 aaaagtctgt ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca
     3541 gcaatatggc gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc
     3601 ccggtttagt gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc
     3661 tgtgctgatt tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacattaac
     3721 ggtgctgggg ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg
     3781 cgatggtatt aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt
     3841 cagcaaatac attttgccgg gtatcggcat tgtgctgggg ctgacagcgg tgttcggtgc
     3901 gctgggctgg atcctgcgcc gtcgtcgcca tcatccgcac cattttggtt acagcctgct
     3961 ggcgctctta ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga
     4021 actggtgaaa tcccagtcac gcgacggcga cccggacttt gcggcttatt ataaagagcc
     4081 gtcgaaaact atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga
     4141 gcggacctat tttgataacg aggctttccc ggacctcacg cctgaactgg gcgcgttgaa
     4201 aaatgaaggc ctggatttca gccacacgca gcagctgccg ggaacagatt acacgattgc
     4261 gggcatggtg gcttctcagt gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc
     4321 cgcttctgtt tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc
     4381 gggttatcag aactatttcg tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt
     4441 cctgaagtcg catggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc
     4501 cgacccgcac tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg
     4561 gaaaaagttt gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt
     4621 cgatacccat cacccggatg gttttatctc tcgtacctgt aaccgcaaaa aatatgattt
     4681 tgacggtaaa ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac
     4741 gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc
     4801 tgaccattta gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa
     4861 cctgtttttt gtcattcgtg gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa
     4921 cacgatggat aacggagcga cagtgctgga cattctcggt ggcgataact atctcggact
     4981 tggtcgtagc agtttatccg ggcagtcaat gtcggaaatc ttcctcaata tcaaagagaa
     5041 aacattggcg tggaagccgg atatcatccg tctgtggaaa ttccctaaag agatgaaaga
     5101 gttcaccatc gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc
     5161 gctgctgttg cgggtttcag acaaacgcgt ggaaccgctg ccagaaagcg aatactcagc
     5221 accgctgcgt ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgaccg
     5281 ttgctacaag atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt
     5341 ctcgcagggg caacttggcg gtcagcaaat tgttcagcat gttgacaaaa caacatggaa
     5401 gagcaagacg gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga
     5461 tacgctgaag attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt
     5521 cgccggtgcg ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg
     5581 ccgctggtcc aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc
     5641 gaagaaattt gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat
     5701 tccggtacgc gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac
     5761 cacactgcat ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc
     5821 tgtctcaacc aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat
     5881 ggtggaaatt aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata
     5941 gggtgcgacc tacacacctt ctccggctta caacacatta ttaagaaaca aaaaaaacag
     6001 cgcccaacac ttccccggac gctgcatggc aactttaata atctcgcctt tcagaaagta
     6061 attacgcctt taattaactc acgattgtta atcacatcgc gctcgtaggt ttctgccagc
     6121 gtggcgaacg gatagcgatg ggttaacatc atgccagcag tgatttttcc ttccgccatc
     6181 agtcgaccga ctttagcaaa atcttccggc gtggcgttgc ggctgcccat catcgtcgtt
     6241 tcttttttat ggaattccgg atcggagaac tgcaactcac ctttaaacag gccgacaaat
     6301 accaccgtgc cgccgtgacg aatcagattc acggtgttat tcatcgcatg ttgattacct
     6361 gtcgcgtcga tcactttctg cgccagcgaa ccaccaaact gcgcccgcag ctgcgcgtca
     6421 aaatcttcgg ctgacgggtc cagcacgggc aactccagac gcgttgccac atgttcacgg
     6481 cgcgccggac tggtatccgc caccaccacc tgtgcgccat cggctttagc gattgccgcc
     6541 gcgcccagac cgattggccc ggccccgacc accagcacct gctcgccggg agcaatggct
     6601 gcgcgacgca ccgcatgagc gctaatagcg aaaggttcaa tcaatgcggc cgcctgcggg
     6661 tcaataccgt ctgcgggcaa aatgttcgcc accggcacgc tcaaatactc actaaaaccg
     6721 ccatcctgat gcacgccaat gactgaaatt ttttcacagc aattggtacg cccgctttta
     6781 cacgccgggc attgctgaca ggcaacataa gggatcacag caacttgctg accattttta
     6841 agatcagcaa tatttttacc cagcccaaca atctccccac atatttcatg gcctaaaacc
     6901 cgtggataac taaaaaatgg ttgatttcca ccccaggcat gaatatcggt accgcaaatc
     6961 ccgacagact taatttttat taatgcttca ttgtcacccg gaataggtat ctcgcgttgt
     7021 ttccagacta attctttcgg ctgctggcaa attaaaacat tcatcgtaga catgattcgg
     7081 ctctccattt tttgttgcct tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa
     7141 atctgttttg tgaaaacaca cgcataaaaa tgttttaaat cgggttttaa tggactcaca
     7201 aggaggaaaa tatgagtcgt tcacaaaatt tacgccacaa tgtgattaac caggtcattg
     7261 atgatatggc gcggggtcat atcccttccc cacttccatc gcaaagtgcg ctggcggaaa
     7321 tgtacaacat cagccgcact acagtgcgtc atattctcag ccacttacgc gaatgcggcg
     7381 tcttaacgca ggttggaaac gactatgtta ttgcgcgtaa acctgaccat gatgacggtt
     7441 tcgcctgtac taccgcttca atgagtgaac aaaacaaagt gtttgagcag gcttttttca
     7501 cgatgataaa ccagcgccag ttacgcccag gggaaacttt ttctgaactg caactggctc
     7561 gggccgcggg tgtcagcccg gtggttgtaa gagaatatct tttaaaattc ggccgttata
     7621 atcttattca aagcgaaaag cgcggccagt ggagcatgaa gcaattcgat caatcctacg
     7681 ctgagcagct gtttgagcta cgcgaaatgc tcgaaacaca ttcacttcag cactttctca
     7741 acctgcctga tcatgatccc cgctggctgc aggcgaaaac catgctcgaa cgtcatcggc
     7801 tactgcgtga caacatcggc aatagctttc gcatgttctc gcaacttgac agagattttc
     7861 actccttact gctttcagct gccgacaata tcttttttga tcaatcgctt gagatcatct
     7921 cagtcatctt tcattttcac tatcaatggg acgaaagcga tcttaaacag cgcaatatca
     7981 ttgccgtcga tgagcatatg accattctca gcgctctgat ttgccgcagt gaccttgatg
     8041 ccacccttgc gctccgtaac catttgaatt cagcgaaaca atcaatgatt cgatccatta
     8101 atgagaacac gcgttatgct cattaagtac ggattaaaaa cagcaaacta ttagcgggtg
     8161 cacggtttta tcaaaagata aataccggga aattcccgct tacctatgct cacaatcagt
     8221 cgactaaaaa tgtttttgtg agcggtagta aagtcctaaa actttaacct gccgttaatg
     8281 tcaaccttgc gtaatgcaca attacgtaaa caattaaaat tataaataca ggagtccggc
     8341 gtggaaaaag aaaatatcac cctcgatccg cgttcttcat ttactccatc ttcgtcggca
     8401 gatattcccg tgccaccaga tggattagtt caacgcagta cccgaattaa acgcattcaa
     8461 accaccgcca tgttgttatt attttttgcg gcggtaatca attatctcga ccgcagttcg
     8521 ctgtcggtag caaatttaac gattcgtgaa gaattgggat taagtgccac cgaaatcggc
     8581 gctttgctct ccgtgttttc actcgcttac ggcattgcgc aacttccttg cggcccacta
     8641 ttggatcgta aaggcccacg cctgatgctg ggactgggga tgttcttctg gtcactgttc
     8701 caggccatgt ctggcatggt gcacagcttt acgcagttcg tgttggtgcg tatcggtatg
     8761 gggattggtg aagcgccgat gaacccatgc ggtgtaaaag tcattaacga ctggttcaac
     8821 atcaaagagc gcggacgccc gatgggcttc ttcaacgcag cttctaccat tggcgttgcc
     8881 gtaagcccac cgattctggc ggcgatgatg ctggtgatgg gctggcgcgg gatgtttatt
     8941 accattggtg tactggggat ttttctcgcc atcggctggt atatgctcta tcgcaaccgc
     9001 gagcacgtag aactgactgc cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc
     9061 cgccgagatc cgctcagttt tgccgaatgg cgcagcctgt tccgtaaccg cacaatgtgg
     9121 ggaatgatgc tcggattcag tggcatcaac tacactgcgt ggctgtatct ggcctggctt
     9181 cctggttacc tgcaaacagc ctataacctg gatttaaaaa gcacagggtt gatggcggct
     9241 atccctttcc tgtttggtgc tgccgggatg ctggtcaacg gttacgttac tgactggctg
     9301 gtcaaagggg gaatggctcc gattaaaagc cgtaagatct gcattattgc cgggatgttc
     9361 tgttctgccg cctttacgct gatagtacca caagcgacaa catccatgat ggcggttctg
     9421 ctgattggca tggcactgtt ctgtattcac tttgccggaa catcctgctg gggcttgatc
     9481 cacgtcgcag ttgcttctcg catgactgcg tcggtgggca gtatccagaa ctttgccagc
     9541 ttcatctgcg cctctttcgc gccgatcatt actggtttta ttgttgatac aacccactca
     9601 ttccgtctgg cactaatcat ttgcggttgc gtcaccgcag cgggagcact ggcgtacatc
     9661 ttcctggttc gtcagccgat caacgacccg cgtaaagatt aaactcccta tgcccgataa
     9721 cattttgctt atcgggcatt tttatggcga ttaaaacgtt tcccagttct ccccgctatc
     9781 tgccactgcc attttacgcg gcgtagctgg cgtcacggtt tttaccacag ccgatgtttc
     9841 acgctgctgt tgctgctgtt gctgaatccg gaacactgcc acagcttcgg tcaggcgact
     9901 ggcctgctct tccaacgcgg cggcggcagc ggcagactct tccaccagcg cagcgttctg
     9961 ttgagttacc cggtccatct cagcaaccgc taagccaacc tgatcgatac cacggctctg
    10021 ctcatcagaa gcagaagcta tttcgcccat aatgtccgtc acgcgggtca cagcactgac
    10081 aatctccgcc attgtttccc cggcgctttc gaccagcgta gagccaacat ccactttccc
    10141 caccgagtct tcaatcaggc ttttaatttc acgagccgcc tgggcgctac gctgggccag
    10201 attacgcact tctcccgcaa ccaccgcaaa accacgtcct tgctcacccg cacgcgctgc
    10261 ttcaaccgcc gcgttcaaag ccagaatatt ggtctggaag gcaatgccgt caattacgct
    10321 gataatatcg gcgattttct gcgaactggt ggagatatca cgcatagtct gcaccacgtt
    10381 atctaccact ttgccgccgc gttgcgccgt ttcagaagca cttaacgcca ggtggctggc
    10441 ctggcgcgca ttctcggcgt tctgtttcac cgttgcggtg agttgctcca tgctggctgc
    10501 cgtctcttcc agcgaagcgg cctgttgctc ggtgcgcgaa gagagatcgt tattgccggt
    10561 agcgatttcg ctggcaccgc tatagatggc attggccccg ttgcgcacat caccgacggt
    10621 acgcatcagc tctccctgca tatggcgcaa actctctgcc agttgcccca tctcattaga
    10681 gccatccacc tcaatcggtt tcaccagatc gccgcctgca atatgacgaa tgctgtcaat
    10741 caggcgattc attggcgcta ccagcgaggc tttaataccg aaccagacgg cgaagatgac
    10801 cgccagtacg acgatcatca cgcccaccag aatccacatc gcctggctgt aggaggcatt
    10861 gttatcgctg acggcgatat catagagccg atcgttttgc tccatgtaag ccacatactg
    10921 cttctcgaaa ccgtcctgat atccctgggt cggctgatca aagaactcgt tgattttgcc
    10981 tgcacctaac agttggatca gctccgccag cgcattgtga taaatatcgt aattacgttt
    11041 gatctctgcc gctgcggcgg tgctctgacg cgggtcacgc ggcaacgctt cgtaatctgc
    11101 ccagtttttt tccgcctgtt tcaacgaaat actggcactc tgcatcagct cagcaacggt
    11161 tgaaccgcta ccaatattat tctgatccat catgtagcgg atacccgcgc ggttgagggt
    11221 gttacgcgtc tgcaacaacg cgacccagct gccattcagc gtggattgct gctggcgaat
    11281 ggtttgtaaa acagtgaaat tttctttgtc attctttaag gcattaaaga acagaccgcc
    11341 tgatgtcagt tgtaaaaggc caaaaacggc caaaaccagc agtaagctgg tcacgatctt
    11401 gatacgtttt aacatggttt ctctttcctg tggaccgatg caaaattttc ggcctgaaat
    11461 gagaaaactt tataggtagc gacttgaaac tggttaattt tacctcactt ttttt
//
LOCUS       DAZKFZ010000141        11513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000141 DAZKFZ010000000
VERSION     DAZKFZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..642)
                     /locus_tag="ACHOQW_003344"
     CDS             complement(<1..642)
                     /locus_tag="ACHOQW_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574370.1"
                     /translation="MYNNLYALTESLFWVDRLLEQEYENIGLNELLVELAEYNKRIDS
                     FIIEQTNSVQESRLNVYMAGNRNIERYKSGLFSMDRVILDDVLYSLSLRYRVNLSENV
                     VEKFIESDSKEKVEHLRKEIARFIRFAKENFDLIRGEFIVFVRRESSIKDSERANGIL
                     EDNSDTNFLFRYLPPNIARLYESRLSVQNIERLGNTNKIRLLNRNVLTNEIMLK"
     gene            1655..1831
                     /locus_tag="ACHOQW_003345"
     CDS             1655..1831
                     /locus_tag="ACHOQW_003345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574371.1"
                     /translation="MNMNEDEIYRHIRQALSSAPRNQYTVELHLQMIKYADELEHITA
                     KAFCEGTGLNTDLL"
     gene            1871..4309
                     /locus_tag="ACHOQW_003346"
     CDS             1871..4309
                     /locus_tag="ACHOQW_003346"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP9574372.1"
                     /translation="MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDK
                     YKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKIIHKLAEENGLKGVIDEADFNDE
                     DKLGKGKEMIDRLSKLVGIFEGLDLSSNRVEGDDLLGDAYEYLMRHFATESGKSKGQF
                     YTPAEVSRILAKVIGITPDTPRDATVYDPTCGSGSLLLKVNDEARRGLSIFGQEMDNA
                     TSALARMNMILHNNATAKIWQGNTLSDPQWKEANGKLKAFDFAVANPPFSNKNWTNGL
                     TPKKDPFERFGWGIPPEKNGDYAFLLHIIKSLKSTGKGAVILPHGVLFRGNAEANIRE
                     NLIKQGYIKGVIGLPANLFYGTGIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKN
                     RLRSQDIHRIVDVFNHQRTVPGYSRMVPSSEIAGNDYNLNIPRYIESGEPEDLHDLTA
                     HLQGGIPVRDVDALKDYWRVFPALRNVLFADDHPGYCRAQVDAQQVKPTILAHQEFKD
                     FATRSLLPFKSWVKEAGLEEIRKGDKPKALIHDISEMLLAQYANSELLNKYSVYQILM
                     DYWADVMQDDVYVIMQDGWKAAAQIRELQPVKGKDGKNIWKETHDFEFTKRRYKADVL
                     PRSLVEIRCFPELLDALNRAQERSEEASRQLVEFIEEQAGEEDLLAEVKNDKDKVTQK
                     LVNARLAQLKPPPADPDELAVLNRCLALIKAEASAKKALKAAQEALDRAVFKHYPTLD
                     EAAIKTLVVQDKWLATLQAGIKAEIERITQQLASRVKELEERYAEPLPALEAAVEALS
                     EKVAGHLRAMGLEW"
     gene            4309..5658
                     /locus_tag="ACHOQW_003347"
     CDS             4309..5658
                     /locus_tag="ACHOQW_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9574373.1"
                     /translation="MVDLMVNEQRSGTAGKDVPAGYKLTEVGVIPEDWVVISIDDCTT
                     KVGSGKTPTGGASVYIDSGRPFVRSQNVDWGRLDLADVVHITDETHNTFSGSEIQQDD
                     VLLNITGASIGRCAKADFRVIGGNVNQHVCIIRTEAKKLSPSLLVELINSNYGQNQID
                     SYQAGGNREGLNFSQVKNLKFAITPNSFEQTEIVRTLNDINAFTLALENLIAKKQAIK
                     AAAMQQLLTGKTRLPQFALRKDGSAKGYKKSELGEIPEDWNCINLGELGNCIIGLTYK
                     PEDTSDYGTLVLRSSNIQNNHLAYDDNVFVSIEPPERTIVKEGDILVCVRNGSRQLIG
                     KCALIDKNAQGSAFGAFMSVFRTDIYGFTFHQFQSDIIQKQIAEVMGATINQITNKDM
                     LGFKIPLPENTEEQAAIAAILSDMDKEIQILQQRLDKTRQLKQGMMQELLTGKIRLI"
     gene            5671..6327
                     /gene="darT"
                     /locus_tag="ACHOQW_003348"
     CDS             5671..6327
                     /gene="darT"
                     /locus_tag="ACHOQW_003348"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system toxin DNA ADP-ribosyl
                     transferase DarT"
                     /protein_id="HFP9574374.1"
                     /translation="MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAEN
                     WINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTPFSPMLMNIHSGRGGIKQRPNEE
                     IVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
                     PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS
                     "
     gene            6324..7391
                     /gene="darG"
                     /locus_tag="ACHOQW_003349"
     CDS             6324..7391
                     /gene="darG"
                     /locus_tag="ACHOQW_003349"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin DNA ADP-ribosyl
                     glycohydrolase DarG"
                     /protein_id="HFP9574375.1"
                     /translation="MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMK
                     VYALACKQKQVITGKMFITETGELMGPRWIVNFPTKQHWRADSRMEWIEDGLQDLRRF
                     LIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
                     GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAH
                     YYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWL
                     PALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEGLHQWPAGERWASRK
                     LRLFDNNNLQFAINRVMEFHC"
     gene            7410..10505
                     /locus_tag="ACHOQW_003350"
     CDS             7410..10505
                     /locus_tag="ACHOQW_003350"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9574376.1"
                     /translation="MSTVGQRERATQQRLIQFFVEDLGYRHLSHRTGYTGNSNIDTNI
                     LSAWLKRRGVNDTLITRALRQLDSAAALGEGKKLYYANKEVYRLLRYGVKDKEGAGEQ
                     NQTVWLIDWANPQANDFAIADEVTVKGEYCKRPDLVLYVNGIALGVIELKRSSVHISE
                     GIRQNLDNQKKTFIRDFFTTMQLVMAGNDTQGLRYGTIETPEKYYLEWKEDNPGDYTH
                     KLDFHLSRLCSKSRLLDIVHNYIAFDVGVKKLCRHNQFFGVEAAKQHIARREGGIIWH
                     TQGSGKSLTMVWLAKWIRENVPNSRVLIVTDRTELDEQIESVFMGVNEDIYRTSSGND
                     LIATLNHPNPWLICSLVHKFGRRSEAEDNAATDAFITELQQSLTKTFRAKGDLFVFVD
                     ECHRTQSGKLHNAMTAILPDALFIGFTGTPLMKKDKKKSVEVFGPYIHTYKFDEAVAD
                     GVVLDLRYEARDIDQYLTSEKKVDDWFEAKTRGLSNLARTQLKQKWGSMQKLLSSKSR
                     LEQIVNDILLDMDTRPRLMDGRGNAMLVCSSIYQACKVYEMFSQTELAGKVAIVTSYR
                     PDAASIKGEETGEGLTEKLSKHATYRKMLADYFEQSEEKAAARITEFEREVKQRFIEE
                     PGQMRLLIVVDKLLTGFDAPSATYLYIDKTMTDHTLFQAICRVNRLDGEDKEYGYIID
                     YKDLFRSLDKAITDYTTGAFDDYDRDDVAGLLKDRLEQAQLDLDSALEMVRSLCEPVK
                     TPRSLQDYQHYFCGESGENQTSLNEKEALRLTFYQCVARLLRAYASIANEMPEAGYTA
                     EEVESIRAEVAEFEKLRYEIKLTSGDLLDMKRFEPAMRHLLDMYVRADDSEVLMDFEE
                     LSLIELIVEKGLAATVALPDDIRNNQDAMAETIENNVRKTIVDENPVNPKYYGRMSQL
                     LDELITLRRQNALNYQQYLERIRDLCKQVIRPEQTAGACYPATMDTQAKRAFYDNFGH
                     DEVLATRIDTTIRYTKRAEWIGDRFKEREIANALREETASYNIDIDEVIALARQQKEY
                     H"
     gene            10505..11221
                     /locus_tag="ACHOQW_003351"
     CDS             10505..11221
                     /locus_tag="ACHOQW_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9574377.1"
                     /translation="MPIMQIGSLHLLVNRKAIKNLHISVLPPDGKVRVSAPEHMTETA
                     IRMAVASRFRWIRKQQQDFARQPRQSEREMVSGECHYLWGRRYRLLVIERHGRHEVKV
                     AGNNKLQLYVQPGTRDENKALALSEFYRHELKQQVAKLLPEWQDRIGVPLTFWGIKKM
                     KTFWGTCNTTTKRIWLNLELIKKPPECLEYILVHELVHLLERHHNARFRLHMDRFIPD
                     WRERRDLLNNMPLAFEAWEY"
BASE COUNT         3179 a         2675 c         2938 g         2721 t
ORIGIN      
        1 tttcaacata atttcattcg ttaaaacatt tctgtttaac aaccgaattt tatttgtgtt
       61 gcccagacgt tctatattct gtacggatag tctactttca tacagacggg caatattagg
      121 ggggagatat cgaaatagga aattagtatc agagttatct tctaaaattc cattagctct
      181 ctccgaatct ttaatagagg attctcttcg tacaaaaacg ataaactctc cacggataag
      241 atcaaagttt tctttagcaa acctgataaa cctagcaatt tccttcctga gatgttcaac
      301 cttctctttg ctatcgcttt caataaattt ttcaactacg ttttctgata gatttaccct
      361 gtatcgtagg ctcaatgaat atagaacgtc atcaagaatg actctgtcca ttgaaaacaa
      421 accactctta tacctttcaa tattacgatt ccccgccatg taaacattca gccgggactc
      481 ctgtacagaa tttgtttgtt cgataatgaa tgaatctatc cttttattat attcagctaa
      541 ttcaactagc aattcgttca atccgatatt ttcatattcc tgttctaaaa gtcgatcaac
      601 ccaaaaaagg ctttctgtga gcgcatagag gttgttgtac atactagctc ctttttacca
      661 agcttcagta ctgtgttctc aagattactc agtggttttc acacagagtg cactctcatt
      721 ttacatttaa atgtacttcc tagcatcctg ctgttagtgc cggattacag cacttatcaa
      781 ctgtatattt agaaaacaaa ctgcgcataa tcctagtgag ttgttatgtc cgctcctcgc
      841 tcaaagcaga ccattcgctc agttaacgca tcagtgtcgt gctaaaagta gacgtggtta
      901 acaacaatgt gagttaatca acggagagca ggtcaactct aacctgtatc aaaaaaacaa
      961 atgtgtcaca aagatgccgc atgccttaat ttgagtataa ttgagacgga agtagacggt
     1021 ttatggctaa atattgttga tatatagcta tttgaagagt tgtagcgcaa gtgtatagcc
     1081 caaactaata acgctacccc ttacctttga cgcctcttat caaagagctg cgatgatgca
     1141 cagcccgcac gaggcaatat tcgcacaaga atcaggttca gaaataacct gctctggcat
     1201 ggtggggaga gtatcactga tcaggctttc gttaagatgc ggcgggatga ggccgtgttg
     1261 cgtgagttgc agcagtacaa gatcgcccgc agagggtaag ctacagcgcg agatcctagg
     1321 tttctatcag ggattcggca aagcgggcgc agttgtcgac caaggcggtg aaatctatag
     1381 tggcattata agggaacgaa caatagatcg agtaaatcgc agtcagggac aaggattgta
     1441 gtctatagag aagtctcctg taacggtttt caggaggcta attccgacgc ctgattttgc
     1501 aggcgcggag ggagaggata ctgcgaccat taatccgcca caaagcattt acgtgcataa
     1561 atcacgcagt cagatccgac tctcactgta gttcttgaac ctgataccac atttagctaa
     1621 tttagctaac ttatccagta taccagtggt tgccatgaat atgaatgaag acgagattta
     1681 tcgtcatatc cggcaggcac tttccagtgc gccgcgtaac cagtacaccg tcgagctgca
     1741 cctgcaaatg attaaatatg ccgacgaact ggaacacatt acggcaaaag ccttttgcga
     1801 aggcaccggt ctgaataccg atctgctgta accgacttac ggcacattat cgcgcaaaac
     1861 gagatattta atggctatca aaaaaaccga actctattct tccctgtggg caagctgcga
     1921 cgaactgcgt ggcgggatgg acgccagcca gtacaaagac tatgtactga ccctgctgtt
     1981 tatgaagtac gtttccgata agtataaagg cgatccttac gggatgatcg tgatacccaa
     2041 aggagccagc tttgacgata tggttgcgct gaaaaatgac aaagagatcg gcgataaaat
     2101 caacaaaatc atccacaagc tggcggaaga gaacggcctg aagggcgtca tcgacgaagc
     2161 cgactttaac gacgaagata aactcggcaa aggcaaggaa atgattgacc gcctgagcaa
     2221 gcttgtcggt atctttgaag ggttagatct ctccagcaac cgggtcgaag gcgacgatct
     2281 gctgggcgat gcctatgaat atctgatgcg tcacttcgca accgaatccg gcaagagtaa
     2341 aggacagttt tacactccgg cggaggtgtc acgtattctg gcaaaggtga ttggtattac
     2401 tcccgatacg ccgcgagatg caacggttta tgacccaacc tgtggctctg gttcgctgct
     2461 gttgaaagtg aatgatgaag cccggcgcgg actgtcgatt ttcggccaag agatggataa
     2521 cgctaccagt gcgctggcgc ggatgaacat gattctgcat aacaatgcca ccgcgaaaat
     2581 ctggcagggc aatacgctga gtgatccgca gtggaaagag gcgaacggca aactgaaagc
     2641 cttcgatttt gccgtggcga acccgccttt ttccaataaa aactggacca acgggctgac
     2701 gccgaaaaaa gatccgtttg aacgcttcgg ctggggtatt ccgccggaaa aaaacggcga
     2761 ttatgctttt ttgttgcata tcattaaaag cctgaaaagt accggcaaag gcgcggtgat
     2821 cctgccccac ggcgtactgt ttcgcggtaa cgccgaagcg aatatccgtg aaaacctaat
     2881 caagcagggc tatatcaaag gcgttattgg tttgccagcc aacctgtttt acggcaccgg
     2941 catcccggcg tgcatcattg tgattgataa agagcacgcc cacagccgca aaggcatttt
     3001 tatgatcgac gccagtcggg gctttatcaa agatggcaac aagaaccgcc tgcgtagcca
     3061 agatattcac cgtattgtgg acgtgttcaa ccatcagcga acggtgccag gctacagccg
     3121 gatggtgccc tccagcgaga tcgccgggaa tgattacaac ctgaatattc ctcgttatat
     3181 cgagagtgga gagccggaag atttgcacga tctgaccgcg cacttacaag gcggtattcc
     3241 agtgcgcgat gtagatgctt tgaaggatta ctggcgggta ttccctgccc tgcgtaatgt
     3301 gctgtttgct gacgaccacc ccggttactg ccgggcgcaa gtggacgcgc agcaggtgaa
     3361 accgaccatt ctggctcatc aggagtttaa ggattttgcc acccgcagcc tgctaccgtt
     3421 taagtcgtgg gtaaaagagg caggtctgga agagatccgc aaaggagata aacccaaagc
     3481 gctaatccac gatatcagcg aaatgctatt ggcgcagtac gccaacagtg agttgctgaa
     3541 caaatacagc gtgtaccaga tcctgatgga ttactgggct gatgtcatgc aggacgatgt
     3601 gtatgtgatt atgcaggatg gctggaaagc cgccgcgcaa atccgcgagc ttcagccggt
     3661 gaaaggcaaa gatggcaaaa acatctggaa agaaacgcac gatttcgaat ttaccaaacg
     3721 gcgctacaag gccgatgtgc tgccccgttc gctggtggaa attcgctgtt tcccggaact
     3781 gctggatgcg ctgaaccgcg cgcaggagcg cagtgaagaa gccagtcgtc agctagtgga
     3841 attcatcgag gagcaggcag gggaagagga tctgctggct gaggtgaaaa acgataaaga
     3901 taaggtgacg caaaagctgg tgaatgcccg tctggcacag ttgaagcccc cccctgccga
     3961 cccggatgag ctggcagtgc tgaaccgctg tctggcgctg attaaggccg aggccagtgc
     4021 gaaaaaagcg ctgaaagccg cacaggaagc gttggatcga gcggtattta aacattatcc
     4081 cacgctggat gaagcagcga ttaaaacgct ggtagtgcag gataagtggc tggcaacgtt
     4141 gcaggcaggg atcaaagcgg agatagagcg tattacccag caactggcgt cgcgcgtaaa
     4201 ggagctggaa gagcgttatg ctgaaccgtt gccagcactt gaagccgccg ttgaggcgtt
     4261 gagcgagaaa gtggccgggc atttgcgggc gatggggctg gagtggtgat ggtggattta
     4321 atggtgaatg agcaacggtc gggtacggcg gggaaagatg tgcctgcggg gtataagctg
     4381 actgaggtgg gggtgattcc tgaggattgg gtagtaatct ctattgatga ctgtacaacc
     4441 aaagttggta gtggaaaaac accaactggt ggagcatctg tttatattga tagcggacgc
     4501 ccttttgtcc gtagccaaaa tgttgattgg ggaagattag atctcgccga tgtcgtacat
     4561 attacagatg aaacccataa cacattctct ggttctgaaa ttcagcaaga tgatgtttta
     4621 ctcaacataa cgggagcatc tatcggccga tgcgctaaag ctgattttag ggttataggt
     4681 ggtaatgtta accaacatgt ttgcatcatc agaacagagg caaaaaaact ctccccatcc
     4741 ttactggttg aattaatcaa ttcaaattat ggacaaaacc agattgatag ttatcaagcc
     4801 ggaggaaaca gagaaggact gaatttttct caagttaaaa atcttaagtt tgctattaca
     4861 ccaaactcct ttgaacaaac ggaaattgtc agaacattaa atgatattaa tgcatttact
     4921 ctcgctctgg aaaacttaat tgctaaaaaa caggccataa aagccgctgc tatgcaacaa
     4981 ctacttaccg gtaaaacccg tttgccacag tttgcgttgc gcaaagatgg ctctgctaag
     5041 ggatataaaa agagcgagtt gggggagatt cctgaagatt ggaattgtat taatcttggt
     5101 gagctaggta attgcattat tgggttaact tacaaacctg aagatacatc tgattatggt
     5161 acattggtat tacgctcatc caacattcaa aataatcact tagcttatga tgataacgtc
     5221 ttcgtcagca tagaaccacc tgagcgcaca attgtgaaag aaggtgatat cttggtatgt
     5281 gtaagaaatg gcagtcgcca acttattggc aaatgcgcat taattgacaa aaatgcgcag
     5341 ggctcagcat tcggtgcatt tatgtcagta ttccgaactg atatatatgg atttacattt
     5401 caccaattcc agtctgatat tattcaaaag caaatcgctg aagttatggg tgccactatc
     5461 aatcagataa ctaacaaaga tatgttgggc tttaaaatcc cattacctga aaatacagaa
     5521 gaacaagccg ccatcgccgc catcctctcc gatatggaca aggaaatcca gatcctgcaa
     5581 cagcgtctgg acaaaacccg ccagcttaag caagggatga tgcaggaact gttaaccggg
     5641 aaaatccgct taatatagaa ggaaaagcac atggcttatg attacagcgc cagcctcaac
     5701 ccgcaaaaag ctctgatctg gcgtatcgtc catcgggaca atatcccgtg gattctggat
     5761 aacgggctgc actgcggcaa cagtctggtg caggcggaaa actggatcaa tatcggcaac
     5821 ccggagctga tcggcaagcg tgccgggcat ccggtacctg tcggaacggg cgggacactg
     5881 catgactatg tgccgtttta ttttaccccc ttctcgccaa tgctgatgaa catccacagc
     5941 gggcgcggcg gcattaagca acgccccaat gaggagatcg ttattctggt cagcaacttg
     6001 cgaaacgtgg cagcacacga cgtacccttc gtgtttaccg acagccatgc ttactataac
     6061 tggaccaact actacaccag tctggatagt ctggaccaga tcgactggcc cattttgcag
     6121 gcacgggact ttcgccgcga tccagacgat ccggctaaat ttgaacgcta tcaggcagag
     6181 gcgctaatct ggcaacactg cccgatctct ctgctggatg ggataatttg ttacagcgaa
     6241 gaggtcaggt tacaattaga acaatggctg tttcagcgta atctaacaat gtccgttcac
     6301 acacgggctg gatggtactt ctcatgatca catatacaca aggcaattta ctggacgcac
     6361 cggtggaagc tctggtgaat acggttaata ccgtcggtgt catggggaaa ggcatcgcgt
     6421 tgatgtttaa agagcgcttc cccgaaaata tgaaagtcta tgccctcgcc tgcaaacaaa
     6481 agcaggtgat caccggcaaa atgttcatta ccgaaaccgg ggaactgatg ggaccgcgct
     6541 ggattgttaa ttttccgacc aaacagcact ggcgcgctga ttcccgcatg gagtggattg
     6601 aagatggctt gcaggactta cgccgctttt tgattgaaga gaacgtgcag tccatcgcca
     6661 ttccaccact gggcgcaggc aacggtggtt tgaactggcc ggacgttcgg gcgcagattg
     6721 aatctgccct tggcgacctt caggacgttg atatcctgat ttaccaaccg actgaaaaat
     6781 accagaacgt tgccaaaagc actggcgtaa aaaagctcac tcccgccaga gccgccatcg
     6841 ctgaactggt gcgtcgctac tgggtattgg ggatggagtg cagcctactg gaaatccaga
     6901 aactggcctg gctgttacaa cgcgccattg agcagcatca acaggacgat atcctcaaac
     6961 tgcggtttga agctcactat tatggcccct atgcgcccaa tctgaaccat ctgcttaatg
     7021 cgctggacgg cacctatctt aaagccgaga aacgcatccc ggacagccaa ccgctggatg
     7081 tgatctggtt taacgatcag aaaaaagaac acgttaatgc gtatctgaac aacgaagcca
     7141 gagagtggct acctgcgctg gagcaggtca gccagttaat tgacggcttt gaatcgccgt
     7201 ttggtctgga gttgctggca accgtagact ggttgctgag ccgtggcgaa tgtcaaccaa
     7261 cgctggattc cgttaaggaa gggctgcacc agtggcctgc gggcgaacgc tgggccagcc
     7321 gtaagttgag gctgtttgat aataataatc tgcaatttgc cattaaccgc gtaatggagt
     7381 tccattgctg agggtttaag gagcgccttt tgagtaccgt gggccagcgc gaacgcgcca
     7441 cacagcagcg tctgattcag ttttttgttg aagatttagg ttaccgccat ctgagtcacc
     7501 gcaccgggta caccgggaac agcaacatcg acactaatat actgagcgcg tggctaaaac
     7561 ggcgcggcgt taatgatacg ttgatcaccc gggcgttgcg gcaactggat tccgccgccg
     7621 ctctgggcga aggcaaaaaa ctctattacg ccaataaaga ggtctatcgc ctactgcgtt
     7681 acggcgtgaa agacaaagaa ggcgcaggcg agcagaacca aaccgtgtgg ctgattgact
     7741 gggccaaccc acaagccaat gatttcgcca tcgccgatga ggtgacggtc aaaggcgagt
     7801 actgcaagcg cccggatctg gtgctgtacg ttaacggcat tgcgctgggc gtgattgaac
     7861 ttaagcgttc gtcagtgcac atcagcgaag gcatccggca gaatctcgat aatcaaaaga
     7921 aaacctttat ccgcgatttc ttcaccacta tgcagttagt gatggcgggc aacgatacgc
     7981 agggcctgcg ctacggcacc atcgaaaccc cggaaaaata ctatctggag tggaaagaag
     8041 ataatccagg cgactataca cataaactcg actttcattt aagtcgtctg tgtagcaaat
     8101 ccaggctgtt ggatatcgtc cataattaca ttgcctttga tgtcggggtg aaaaaactct
     8161 gccgccataa tcagttcttc ggcgtcgagg cggctaaaca acatattgcc cgtcgtgaag
     8221 gcggtattat ctggcacaca cagggatcgg gtaaaagcct gacgatggtc tggctggcga
     8281 agtggatccg tgaaaatgtg ccaaactccc gcgtgttgat tgtgactgac cgcaccgaac
     8341 tggatgaaca gatcgaaagc gtgtttatgg gcgtgaatga agatatttat cgcacctcca
     8401 gcggcaacga tcttatcgca acgctcaatc atcctaaccc gtggctcatt tgctcgctgg
     8461 tgcataaatt tggtcgtcgc agcgaagctg aagacaatgc ggctaccgac gcctttatca
     8521 ccgaattaca gcagtccctg acgaaaacat tccgcgccaa aggcgatctg tttgtttttg
     8581 tggacgagtg ccaccgcaca caatccggca agctgcataa cgccatgact gccattctgc
     8641 cggacgccct gtttatcggt tttaccggca caccgctgat gaagaaagat aagaagaagt
     8701 cggttgaggt gttcggccct tatatccata cctataaatt tgatgaagcg gtggccgacg
     8761 gtgtggtgct ggatctgcgc tacgaggcac gcgatattga ccagtatctg acatcagaga
     8821 aaaaggtcga tgactggttt gaagccaaga ctcgcggttt gtcgaattta gccaggaccc
     8881 agcttaagca aaagtgggga tcaatgcaga agctgctctc cagcaagtcg cggctggagc
     8941 agatcgttaa cgacattttg ctggatatgg atacccgtcc acgcctgatg gacgggcgcg
     9001 gcaacgccat gctggtatgc agcagtatct atcaagcctg caaggtttac gagatgttca
     9061 gccagaccga gctggcagga aaagtagcga ttgtgaccag ctatcgcccc gatgccgcca
     9121 gcatcaaagg cgaagaaacg ggcgaagggt taacggagaa actctctaaa cacgccacct
     9181 atcgtaaaat gttggctgac tattttgaac aaagtgaaga gaaggccgcc gcacgcatta
     9241 ccgagttcga gcgggaagtg aaacagcgtt ttattgaaga gccggggcaa atgcgcctgc
     9301 ttatcgtggt ggataagctg ctgactggct ttgacgcgcc gtccgccacc tatctctaca
     9361 ttgataaaac catgaccgac catacgctgt ttcaggcgat ttgtcgggtg aaccggctgg
     9421 acggtgagga taaagagtat ggctatatta tcgattataa agatctgttc cgttcgctgg
     9481 ataaagcgat taccgattac accacgggcg cgtttgatga ttacgatcgg gacgatgtgg
     9541 ccgggttgct gaaagacagg ctggagcagg cgcaactgga tctcgacagc gcgctggaga
     9601 tggttcgcag cttatgtgaa cccgtcaaaa ccccgcgcag cctgcaggac tatcagcact
     9661 atttttgcgg tgaatcagga gagaatcaga cttcgttaaa cgaaaaagag gcgctgcgcc
     9721 tgacatttta ccagtgcgtc gcccgtctgc tacgcgctta tgccagcatc gccaatgaaa
     9781 tgccggaagc aggctacact gccgaagagg tagagagcat tcgcgccgaa gtcgctgaat
     9841 tcgaaaaact gcgctatgag attaagctga ccagcggcga tcttctggat atgaagcgct
     9901 ttgaacccgc tatgcgtcat ttactggata tgtatgttcg cgccgatgat agcgaagtgc
     9961 tgatggattt tgaagagtta agtctgattg aactgattgt cgagaaaggc ttggcggcga
    10021 ccgtcgccct gccggatgat atccgtaata atcaggatgc gatggcggaa accatcgaaa
    10081 acaacgtgcg caaaaccatc gtggatgaaa acccggttaa cccaaaatac tatggacgca
    10141 tgtcgcagct gctggatgaa ctgattaccc ttcgccgaca aaatgcgctc aattatcagc
    10201 agtatctgga acgtatccgc gatttgtgta aacaagtcat ccgcccggag caaaccgccg
    10261 gagcgtgcta ccctgccacc atggatacac aagcaaagcg cgccttctat gataacttcg
    10321 gccacgacga ggtactggcg acccgcatag atacgacgat tcgctatacc aaaagggcag
    10381 agtggattgg cgatcgtttt aaagaacggg aaattgccaa cgcattacgt gaagagaccg
    10441 ccagctataa tattgatatt gatgaggtga tagcgcttgc ccgtcagcag aaggaatatc
    10501 actgatgcca atcatgcaaa taggctcgtt acacttgctg gtcaaccgta aggcgattaa
    10561 gaatctgcat atcagcgtgc tgccgcctga tggcaaagtc cgcgtttcag cgccggaaca
    10621 tatgacggag acggcgattc gtatggcggt tgcttcacgc tttcgctgga tcagaaaaca
    10681 gcagcaggat ttcgccagac agcctcgtca atcagagagg gaaatggtca gcggagagtg
    10741 ccactatctt tggggccgaa gatatcgcct acttgtgatt gagcgtcacg ggcgtcacga
    10801 agtaaaagtt gcgggaaata ataagctcca gctttatgtt caaccgggaa cccgtgatga
    10861 aaataaggca ttggcgttaa gtgaatttta tcgacatgaa ctaaaacagc aggtggctaa
    10921 gcttctacct gaatggcagg accgaatcgg tgttccccta acattctggg gcatcaaaaa
    10981 gatgaaaacc ttttggggca cctgtaacac tacaacaaaa cgaatctggc tcaatctgga
    11041 gctaattaag aagccgccgg agtgtctgga gtatattctg gtgcatgaac tggttcatct
    11101 tctggaacgt caccataatg ctcgcttccg gttgcatatg gatcggttta ttcctgactg
    11161 gcgtgagcgt cgggatttgt taaataatat gccgttagcg tttgaggcat gggagtattg
    11221 attgagcctg taactgtttt ttgtccataa attaaccttc atttgatgct ggattgaaca
    11281 tatcaaaatc agacaattac acaaatctat gtacaggctc aactcctgcc atggaacgta
    11341 gtactcacca ataagtaagc atcaatacgg cgctccgtta acgcttacta gcatggttat
    11401 ctccttaaaa aattgaggcg acaactatgc tgacacaggg ggttactcac cgatctcctt
    11461 cacggtaata cgcagttctt cagaacccgc ctccgatacc agcctggcgg tcg
//
LOCUS       DAZKFZ010000142        11510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000142 DAZKFZ010000000
VERSION     DAZKFZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            290..589
                     /gene="yoaC"
                     /locus_tag="ACHOQW_003352"
     CDS             290..589
                     /gene="yoaC"
                     /locus_tag="ACHOQW_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP9574378.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(709..888)
                     /gene="yoaH"
                     /locus_tag="ACHOQW_003353"
     CDS             complement(709..888)
                     /gene="yoaH"
                     /locus_tag="ACHOQW_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP9574379.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            962..2323
                     /gene="pabB"
                     /locus_tag="ACHOQW_003354"
     CDS             962..2323
                     /gene="pabB"
                     /locus_tag="ACHOQW_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP9574380.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2327..2905
                     /gene="nudL"
                     /locus_tag="ACHOQW_003355"
     CDS             2327..2905
                     /gene="nudL"
                     /locus_tag="ACHOQW_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP9574381.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3089..4453
                     /gene="sdaA"
                     /locus_tag="ACHOQW_003356"
     CDS             3089..4453
                     /gene="sdaA"
                     /locus_tag="ACHOQW_003356"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9574382.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4584..6182
                     /gene="pdeD"
                     /locus_tag="ACHOQW_003357"
     CDS             4584..6182
                     /gene="pdeD"
                     /locus_tag="ACHOQW_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9574383.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6186..7742)
                     /gene="yoaE"
                     /locus_tag="ACHOQW_003358"
     CDS             complement(6186..7742)
                     /gene="yoaE"
                     /locus_tag="ACHOQW_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP9574384.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7730..7888)
                     /gene="yoaL"
                     /locus_tag="ACHOQW_003359"
     CDS             complement(7730..7888)
                     /gene="yoaL"
                     /locus_tag="ACHOQW_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP9574385.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8205..9176
                     /gene="manX"
                     /locus_tag="ACHOQW_003360"
     CDS             8205..9176
                     /gene="manX"
                     /locus_tag="ACHOQW_003360"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP9574386.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9239..10039
                     /gene="manY"
                     /locus_tag="ACHOQW_003361"
     CDS             9239..10039
                     /gene="manY"
                     /locus_tag="ACHOQW_003361"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP9574387.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10052..10903
                     /gene="manZ"
                     /locus_tag="ACHOQW_003362"
     CDS             10052..10903
                     /gene="manZ"
                     /locus_tag="ACHOQW_003362"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP9574388.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10958..11416
                     /gene="yobD"
                     /locus_tag="ACHOQW_003363"
     CDS             10958..11416
                     /gene="yobD"
                     /locus_tag="ACHOQW_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP9574389.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
BASE COUNT         2835 a         2892 c         2872 g         2911 t
ORIGIN      
        1 tttatctata aaactaacta tataaataaa cataaccatg ccgttgtgtt cgccctttga
       61 ttagcaactc tggttttctt tcttagctga acaatctggt tatagcgtgc ggactgaccg
      121 ttaattttct ccatcttact ggataacaca ccatgagctg ctacctttaa aacacctgaa
      181 tatcaacagg ttaactcgtg ccatatcgtt ataatccctt tgatattaga tgcaaattaa
      241 ggtcatatat agccttatcg ataacatggt taattttaag gataagaata tgcctgctgt
      301 aatagataaa gccctggatt tcattggtgc catggatgta tcagcgccaa caccaagttc
      361 gatgaatgaa agcacggcga agggaatctt taaatattta aaagaactgg gagtacccgc
      421 gagtgccgct gatattacgg cgcgagctga ccaggaaggc tggaaccctg ggttcacgga
      481 aaaaatggtt ggatgggcaa aaaaaatgga gacaggtgaa cgctctgtga ttaaaaatcc
      541 tgagtacttt tcaacatata tgcaggaaga actaaaagca ctggtctgag ttaaatttat
      601 atcagcataa atgggtcagg acgcttttaa tcacatataa aaagcgtcct ttttctcaca
      661 atcaaataat catcagcttc tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc
      721 atcctcaaaa cgcgccacga ttcgctcgcc ggagtggttg gcacgcagct cttctgccac
      781 cagcgcgatt gcctggccgc tgctcatccc ttgtgccatc agttcctgga tccgctcgac
      841 agctttttgc tgctgttcat gggtgagtga aggtaaacct gcaaacattg ttaactcctg
      901 ctaaattgtt ggcgctaatt atttcatgct acccggcaca tagccagtag agtcaggact
      961 gatgaagacg ttatctcccg ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt
     1021 ttatttctcc cgcttaagcc acctgccgtg ggcgatgctt ttacactccg gctatgccga
     1081 tcatccgtat agccgctttg atattgtggt cgccgatccg atttgcactt taaccacttt
     1141 aggtaaagaa accgttgtta gtgaaagcga aaaacgcaca acgaccactg atgacccgct
     1201 acaggtgctc cagcaggtgc tggatcgcgc agacattcgc ccaacgcata acgaagattt
     1261 gccatttcag ggcggcgcac tggggttgtt tggctacgat ctgggccgcc gttttgagtc
     1321 actgccagaa attgcggaac aagatatcgt tctgccggat atggcagtgg gtatctacga
     1381 ttgggcgctc attgtcgacc accagcgtca tacagtttct ttgctgagtc ataatgatgt
     1441 caatgcccgt cgggcctggc tggaaagcca gcaattctcg ccgcaggaag atttcacgct
     1501 cacttccgac tggcaatcca atatgacccg cgagcagtac ggcgaaaaat ttcgccgggt
     1561 acaggaatat ctgcacagcg gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc
     1621 gacctattct ggcgatgaat ggcaggcatt ccttcagctt aatcaggcca accgcgcgcc
     1681 atttagcgct tttttacgtc ttgaacaggg tgcaatttta agcctttcgc cagagcggtt
     1741 tattctttgt gataatagtg aaatccagac ccgcccgatt aaaggcacgc taccacgcct
     1801 gcccgctcct caggaagata gcaaacaagc agaaaaactg gcgaactcag cgaaagatcg
     1861 tgccgaaaat ctgatgattg tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc
     1921 aggttcggta aaagtaccag agctgttcgt ggtggaaccc ttccctgccg tgcatcatct
     1981 ggtcagcacc ataacggcgc aactaccaga acagttacac gccagcgatc tgctgcgcgc
     2041 agcttttcct ggtggctcaa taaccggggc tccgaaagta cgggctatgg aaattatcga
     2101 cgaactggaa ccgcagcgac gtaatgcctg gtgcggcagc attggctatt tgagcttttg
     2161 cggcaacatg gataccagca ttactatccg cacgctgact gccattaacg gacaaatata
     2221 ctgctctgcg ggaggtggaa ttgtcgccga tagccaggaa gaagcggaat atcaggaaac
     2281 ttttgataaa gttaataaga tattacgcca actggagaag taagacgtgg aataccgtag
     2341 cctgacgctt gatgattttt tatcgcgctt tcaacttttg cgcccgcaaa ttaaccggga
     2401 aaccctaaat catcgtcagg ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg
     2461 gttgttgctg actcagcgtt caattcatct gcgtaaacac gctggacaag tggcattccc
     2521 tggaggtgca gtcgatgaca cggacgcatc agttatcgcc gccgcgctgc gcgaagctga
     2581 agaagaggtt gctataccgc cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga
     2641 tagcgtaact ggctaccagg taaccccagt ggtcggcatt atcccgcccg atctgccgta
     2701 tcgcgccagt gaagatgaag tttccgctgt gtttgaaatg ccgctcgccc aggcattaca
     2761 tctgggtcgt tatcaccctt tagatatcta ccgccgcggc gattcacatc gggtatggct
     2821 atcctggtac gaacagtatt ttgtatgggg aatgaccgca ggcataattc gtgagctggc
     2881 gctgcaaatt ggtgtgaaac cctgactata cttatcttta catctacaaa acactacttg
     2941 agacaatcat cgcaatatta gttaaatcgc ggtttttgat tagtttaatt catgtgaata
     3001 gttaagccag tcgccgcgtt ccctcttaca ctatgcgctg ttattagttc gttactggaa
     3061 gtccagtcac cttgtcagga gtattatcgt gattagtcta ttcgacatgt ttaaggtggg
     3121 gattggtccc tcatcttccc ataccgtagg gcctatgaag gcgggtaaac agttcgtcga
     3181 tgatctggtc gaaaaaggct tactggatag cgttactcgc gttgccgtgg acgtttatgg
     3241 ttcactgtcg ctgacgggta aaggccacca caccgatatc gccattatta tgggtcttgc
     3301 aggtaacgaa cctgccaccg tggatatcga cagtattccc ggttttattc gcgacgtaga
     3361 agagcgcgaa cgtctgctgc tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa
     3421 cgggatgcgt tttcataacg gcaacctgcc gctgcatgaa aacggtatgc aaatccacgc
     3481 ctataacggc gatgaagtcg tctacagcaa aacttattat tccatcggcg gcggttttat
     3541 cgtcgatgaa gaacactttg gtcaggatac tgccaacgaa gtgagcgtgc cgtatccgtt
     3601 caaatctgcc accgaactgc tcgcgtactg taatgaaacc ggctattcgc tgtctggtct
     3661 cgctatgcag aacgaactgg cgctgcacag caagaaagag atcgacgagt atttcgcgca
     3721 tgtctggcaa accatgcagg catgtatcga tcgcgggatg aacaccgaag gtgtactgcc
     3781 aggcccgctg cgcgtgccac gtcgtgcgtc tgccctgcgc cggatgctgg tttccagcga
     3841 taaactgtct aacgatccga tgaatgtcat tgactgggta aacatgtttg cgctggcagt
     3901 taacgaagaa aacgccgccg gtggtcgtgt ggtaactgcg ccaaccaacg gtgcctgcgg
     3961 tatcgttccg gcagtgctgg cttactatga ccactttatt gaatcggtca gcccggacat
     4021 ctatacccgt tactttatgg cagcgggcgc gattggtgca ttgtataaaa tgaacgcctc
     4081 tatttccggt gcggaagttg gttgccaggg cgaagtgggt gttgcctgtt caatggctgc
     4141 tgcgggtctt gcagaactgc tgggcggtag cccggaacag gtttgcgtgg cggcggaaat
     4201 tggcatggaa cacaaccttg gtttaacctg cgacccggtt gcagggcagg ttcaggtgcc
     4261 gtgcattgag cgtaatgcca ttgcctctgt gaaggcgatt aacgccgcac ggatggctct
     4321 gcgccgcacc agtgcaccgc gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac
     4381 cggtaaggac atgaacgcca aataccgcga aacctcacgc ggtggtctgg caatcaaagt
     4441 ccagtgtgac taatacttct tactcgccca tctgcaacgg atgggcgaat ttatccccgc
     4501 tttctcgtct gctgtaatat tccccactac acttccactg ttgcgtcagg cgtttgtcgc
     4561 catacgctta cagagtggcc cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc
     4621 gtaatcgaat gattgtttgt acgatttgcg cactcgttac gctcgcttcg accctgagcg
     4681 tgcgatttat ttcacagcgt aacttaaatc aacaacgggt agtacaattc gccaatcacg
     4741 ctgtagagga attagataaa gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc
     4801 cgctgattgg tctgccctgc tctgtcgccc atttgccatt acgtaaacag gcggcaaaac
     4861 tccaaactgt gcgatccatt ggcctggtgc aagacggcac actttattgc tccagcattt
     4921 ttggttatcg caatgtgccc gtcgtggaca ttctggctga acttcctgca ccgcaaccac
     4981 ttttacgcct gacgaccgac cgtgtcctga ttaaaggcag tccggttttg attcaatgga
     5041 cgcctgcagc gagcagtagc aatgctgggg tcatggagat gattaacatc gacttactga
     5101 cggcaatgct gcttgagcca caactgccgc aaatcagtag cgccagcctg acggtggaca
     5161 aacggcattt gctctatggt aatgggctgg tagattccct tccacaacct gaagacaatg
     5221 aaaactatca ggtttcttcg caacgctttt cttttaccat taacgttaat ggtccggggg
     5281 ctccggcgct ggcatggcac tatcttccaa cacaattacc gctggcggta ctgctaagtt
     5341 tactggtggg ctacatcgcc tggctggcga ccgcttaccg aatgagcttt tcccgcgaaa
     5401 tcaatctggg cctggcgcaa catgagttcg aattgttctg tcagcctttg cttaatgcgc
     5461 gcagccagca atgtattggt gtagagattt tgctacgctg gaacaatccg cgtcagggct
     5521 ggatttcacc ggatgtgttt attcctatcg cggaagaaca tcatttaatt gtgccactga
     5581 cccgctatgt gatggcagaa accattcgtc agcgccatgt tttcccgatg agtagtcagt
     5641 ttcatgttgg cattaacgtc gcacccagcc attttcgccg tggtgtgctg ataaaagatc
     5701 tcaatcagta ctggtttagc gctcacccga ttcagcaact gatcctcgaa atcaccgaac
     5761 gcgatgcctt actggatgtt gattatcgga ttgcccgcga gctacatcgt aaaaacgtca
     5821 aactggcgat tgatgacttc ggcaccggca acagttcatt ttcctggctt gaaacattac
     5881 gtcctgacgt gctgaaaatt gataagtcat ttaccgcagc tataggttct gacgcggtta
     5941 actcgacggt gaccgatatc atcatcgcgc tggggcaaag actgaatatt gaactggtgg
     6001 cggagggcgt ggaaacgcaa gaacaggcga agtatttgcg ccgtcatggc gtgcatattt
     6061 tgcaagggta tttgtacgca cagccgatgc cgctacgtga ttttcccaaa tggctggcgg
     6121 gcagccaacc gccgcccgcc cggcataatg gacatatcac gcccattatg ccgttacgtt
     6181 aacgcttact catcttcatc gtgcgccggt tgctctttaa caatgcgaac cagatcaaca
     6241 cgataatcat tggcttcaat gatggtgata tgcagtggcc ctacatcaat cacatcgccc
     6301 acacggggaa tgtgaccatt tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg
     6361 tcatcggcaa ggtgctcaac atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct
     6421 tttaccagcc agccgtcacc atcagtaatg atttccggcg tttcgtcagc gtccgggaat
     6481 tcacccgcaa tggcttccag cacatccagc ggcgtgacca agccttgtac cacaccaaac
     6541 tcgttggtca cgataacaaa gctcccgcga gcacgacgca gcacgcccag caggttgatc
     6601 ggatcgaggg tttcagggac gataatcgcc ggagacgccg aagcaatcgc cgccacatca
     6661 acgccctctt ccagcgccac cagcagttct ttagcacgta cgattccgat gatttcatcc
     6721 agttcaccgc gacataccgg gaacaggctg tgcggtgagg agagcagctg ctcgcggatt
     6781 tcatcgaccc cgagattagc gtcaacccag cttatttcac cgcgcggcgt catgatcccg
     6841 cgcagagaac gcgacgccag cgtcagtacg ccgttaatca tgtaacgttc ttcttcggca
     6901 aatgcacctt ccgggatcgg catcggcatc gggttatcgg catcgtgctg aacattggcc
     6961 tgacgtttcc cgcccatcaa acgcaggatg gcatcggcag tacgcgctcg cagcggcaaa
     7021 gtcgactggt gacgaataaa gttgcgacgc gcaatctggt taaacacttc gatgatgatc
     7081 gagaagccaa tcgcggcata caggtaacct ttcggaatgt ggaaaccgaa accttctgcc
     7141 accagactca gaccaatcat taacaggaag ctcagacaga gcaccaccac cgtggggtgc
     7201 tggttaacga atcgcgtcag cggtttggat gccagcaaca taaccgccat cgcaatcact
     7261 accgccgcca tcatcaccgg cagatggtta accatcccta ctgcagtaat taccgcatcc
     7321 aacgagaaga cggcgtcaag gatgacaatc tgtgtgacga ccacccagaa actggcgtag
     7381 cctttaccgt ggccggaatc atgatcgcgg ttttccagcc gttcatgcag ttcggttgtt
     7441 gctttgaaca gcaagaatat ccccccgaac aacataatca ggtcgcgtcc ggagaaggag
     7501 aaatccatga cggtaaatag cggtttggtc agcgtgacca tccatgaaat cagcgacagc
     7561 agccccagac gcataatcag cgccagtgat aaccccagca aacgcgcttt atcgcgttgt
     7621 tttggcggca gtttgtcagc aagaatggcg atgaagacca ggttatcgat acccagcaca
     7681 atttcgagaa caacaagcgt gagtagcccc gcccaaattg aggggtccat taagaattcc
     7741 atgacaagct cctgcttaag gaatagctat tcgacgccag aaataatgca ggcgtaacga
     7801 caaaatgcaa acgaaaggtg cggcatagag tgccagaaag gcaggcgtta aaaggcctga
     7861 tgctgaaatg acgtcggtga cgatccatac tgcgggctac tgccctatac tccatgattg
     7921 ttaaacggga gttaaacata tcagagacgc ctctgatttg gcaaagattt accttccttt
     7981 gcaaacgaat gtgacaagga tattttacct ttcgaaattt ctgctaatcg aaagttaaat
     8041 tacggatctt catcacataa aataattttt tcgatatcta aaataaatcg cgaaacgcag
     8101 gggtttttgg ttgtagccct tatctgaatc gattcgattg tggacgacga ttcaaaaata
     8161 catctggcac gttgaggtgt taacgataat aaaggaggta gcaagtgacc attgctattg
     8221 ttataggcac acatggttgg gctgcagagc agttgcttaa aacggcagaa atgctgttag
     8281 gcgagcagga aaacgtcggc tggatcgatt tcgttccagg tgaaaatgcc gaaacgctga
     8341 ttgaaaagta caacgctcag ttggcaaaac tcgacaccac taaaggcgtg ctgtttctcg
     8401 ttgatacatg gggaggcagc ccgttcaatg ctgccagccg cattgtcgtc gacaaagagc
     8461 attatgaagt cattgcaggc gttaacattc caatgctcgt ggaaacgtta atggctcgtg
     8521 atgatgaccc aagctttgat gaactggttg cgctggcagt agaaacaggc cgtgaaggcg
     8581 tgaaagcact gaaagccaaa ccggttgaaa aagccgcgcc agcacccgct gccgcagcac
     8641 ccaaagcggc tccaactccg gcaaaaccaa tggggccaaa cgactacatg gttattggcc
     8701 ttgcgcgtat cgacgaccgt ctgattcacg gtcaggtcgc cacccgctgg accaaagaaa
     8761 ccaatgtctc ccgtattatt gttgttagtg atgaagtggc tgcggatact gttcgtaaga
     8821 cactgctcac ccaggttgca cctccgggcg taacagcaca cgtagttgat gttgccaaaa
     8881 tgattcgcgt ctacaacaac ccgaaatatg ctggcgaacg tgtaatgctg ttatttacca
     8941 acccaacaga tgtagagcgt ctcgttgaag gcggcgtgaa aatcacctct gttaacgtcg
     9001 gtggtatggc attccgtcag ggtaaaaccc aagtgaataa cgcggtttcg gttgatgaaa
     9061 aagatatcga ggcgttcaaa aaactgaatg cacgcggtat tgagctggaa gtccgtaagg
     9121 tttccaccga tccgaaactg aaaatgatgg atctgatcag caaaatcgat aagtaacgta
     9181 ttgtgttgat tatcagtcag ttttcacact ttagtcatac gtaaacagga gaagtacaat
     9241 ggagattacc actcttcaaa ttgtgctggt atttatcgta gcctgtatcg caggtatggg
     9301 atcaatcctc gatgaatttc agtttcaccg tccgctaatc gcgtgtaccc tggtgggtat
     9361 cgttcttggg gatatgaaaa ccggtattat tatcggtggt acgctggaaa tgatcgcgct
     9421 gggctggatg aacatcggtg ctgcagttgc gcctgacgcc gctctggcat ctatcatttc
     9481 taccattctg gttatcgcag gtcatcagag cattggtgca ggtattgcac tggcaatccc
     9541 tctggccgct gcgggccagg tactgaccat catcgttcgt actattaccg ttgctttcca
     9601 gcacgctgcg gataaggctg ctgataacgg caacctgaca gcgatttcct ggatccacgt
     9661 ttcttctctg ttcctgcaag caatgcgtgt ggctattccg gccgtcatcg ttgcgctgtc
     9721 tgtaggtacc agcgaagtac agaacatgct gaatgcgatt ccggaagtgg tgaccaatgg
     9781 tctgaatatc gccggtggca tgatcgtggt ggttggttat gcgatggtta tcaacatgat
     9841 gcgtgctggc tacctgatgc cgttcttcta cctcggcttc gtaaccgcag cattcaccaa
     9901 ctttaacctg gttgctctgg gtgtgattgg tactgttatg gcagtgctct acatccaact
     9961 tagcccgaaa tacaaccgcg tagccggtgc gcctgctcag gcagctggta acaacgatct
    10021 cgataacgaa ctggactaac aggtgagcga aatggttgat acaactcaaa ctaccaccga
    10081 gaaaaaactc actcaaagtg atattcgtgg cgtcttcctg cgttctaacc tcttccaggg
    10141 ttcatggaac ttcgaacgta tgcaggcact gggtttctgc ttctctatgg taccggcaat
    10201 tcgtcgcctc taccctgaga acaacgaagc tcgtaaacaa gctattcgcc gtcacctgga
    10261 gttctttaac acccagccgt tcgtggctgc gccgattctc ggcgtaaccc tggcgctgga
    10321 agaacagcgt gctaatggcg cagagatcga cgacggtgct atcaacggta tcaaagtcgg
    10381 tttgatgggg ccgctggctg gtgtaggcga cccgatcttc tggggaaccg tacgtccggt
    10441 atttgcagca ctgggtgccg gtatcgcgat gagcggcagc ctgttaggtc cgctgctgtt
    10501 cttcatcctg tttaacctgg tgcgtctggc aacccgttac tacggcgtag cgtatggtta
    10561 ctccaaaggt atcgatatcg ttaaagatat gggtggtggc ttcctgcaaa aactgacgga
    10621 aggggcgtct atcctcggcc tgtttgtcat gggggcattg gttaacaagt ggacacatgt
    10681 caacatcccg ctggttgtct ctcgcattac tgaccagacg ggcaaagaac acgttactac
    10741 tgtccagact attctggacc agttaatgcc aggcctggta ccactgctgc tgacctttgc
    10801 ttgtatgtgg ctactgcgca aaaaagttaa cccgctgtgg atcatcgttg gcttcttcgt
    10861 catcggtatc gctggttacg cttgcggcct gctgggactg taagactgtt gtacactacc
    10921 ggggcctttt ggccccgttt ttttatctgg aggattaatg acaatcacgg acctggtact
    10981 gattcttttc atcgccgcac tcctggcctt cgcgatctac gatcagttca tcatgccccg
    11041 ccgtaacggc cccaccctgc tggcaattcc tttgctccgg cgtggtcgca tcgatagcgt
    11101 tatcttcgtc ggattgattg tcattcttat ctataacaac gtcacgaatc atggtgcgtt
    11161 aataacgaca tggttattaa gcgcactggc tctgatgggt ttttatatat tctggatccg
    11221 cgttccgaag atcatcttta aacaaaaagg ttttttcttc gccaatgtct ggattgaata
    11281 tagccgaatc aaagcgatga acttgtcgga agatggcgtg ttggtgatgc aattagaaca
    11341 gcgtcggctg ttaatccgcg ttcgaaatat cgacgatctg gaaagaattt ataaacttct
    11401 cgtttcaact caatgagtta ttaatttagc caaagctatg tttagtgtat ttttaataat
    11461 cagacatagc ttaggctata ttacctcttc ccttatttgt tatttattat
//
LOCUS       DAZKFZ010000143        11487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000143 DAZKFZ010000000
VERSION     DAZKFZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            358..1224
                     /gene="folD"
                     /locus_tag="ACHOQW_003364"
     CDS             358..1224
                     /gene="folD"
                     /locus_tag="ACHOQW_003364"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP9574390.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1226..1438
                     /gene="ybcJ"
                     /locus_tag="ACHOQW_003365"
     CDS             1226..1438
                     /gene="ybcJ"
                     /locus_tag="ACHOQW_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP9574391.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1546..2067
                     /locus_tag="ACHOQW_003366"
     CDS             1546..2067
                     /locus_tag="ACHOQW_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9574392.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2103..3488)
                     /gene="cysS"
                     /locus_tag="ACHOQW_003367"
     CDS             complement(2103..3488)
                     /gene="cysS"
                     /locus_tag="ACHOQW_003367"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9574393.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3662..4156
                     /gene="ppiB"
                     /locus_tag="ACHOQW_003368"
     CDS             3662..4156
                     /gene="ppiB"
                     /locus_tag="ACHOQW_003368"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP9574394.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4159..4881
                     /gene="lpxH"
                     /locus_tag="ACHOQW_003369"
     CDS             4159..4881
                     /gene="lpxH"
                     /locus_tag="ACHOQW_003369"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP9574395.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4999..5508
                     /gene="purE"
                     /locus_tag="ACHOQW_003370"
     CDS             4999..5508
                     /gene="purE"
                     /locus_tag="ACHOQW_003370"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9574396.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5505..6572
                     /gene="purK"
                     /locus_tag="ACHOQW_003371"
     CDS             5505..6572
                     /gene="purK"
                     /locus_tag="ACHOQW_003371"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9574397.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6711..7604)
                     /locus_tag="ACHOQW_003372"
     CDS             complement(6711..7604)
                     /locus_tag="ACHOQW_003372"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9574398.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7601..8416)
                     /locus_tag="ACHOQW_003373"
     CDS             complement(7601..8416)
                     /locus_tag="ACHOQW_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9574399.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8427..9686)
                     /locus_tag="ACHOQW_003374"
     CDS             complement(8427..9686)
                     /locus_tag="ACHOQW_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9574400.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9696..11324)
                     /locus_tag="ACHOQW_003375"
     CDS             complement(9696..11324)
                     /locus_tag="ACHOQW_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9574401.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLANQALDRNLNVMMFSDNVTLEDEIQLKTRAREKGLLVMGP
                     DCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGEGITHAIGLGGRDL
                     SREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMKATGKPTVALFLGY
                     TPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFICGLYTGGTLAAEAA
                     GLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMIDPTLRNQLITDLG
                     AKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYAIATVTGTERDPQC
                     RSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLLENVAVINIGLRSF
                     ALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2586 a         3253 c         3013 g         2635 t
ORIGIN      
        1 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa
       61 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
      121 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
      181 ccatcatgga cgaaaaatga agctttcaat ctcagcgacc tcagcgacag tgcgcctaac
      241 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
      301 tctgacaaaa taggcgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
      361 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
      421 aaagttcagg cgcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
      481 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gtaaggcttg tgaagaagtc
      541 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
      601 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
      661 ccggcgggta ttgataacgt caaagtgctg gaacgtatac acccggacaa agacgtagac
      721 ggtttccatc cttataacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
      781 accccgcgcg gtattgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
      841 gccgtggtga ttggcgcatc gaatatcgtt ggccgcccga tgagcatgga actgttgctc
      901 gcaggttgca ccaccaccgt gactcaccgt ttcactaaaa atctgcgtca ccacgtagaa
      961 aatgccgatc tgttgatcgt tgccgtcggc aagccgggct ttattcctgg tgactggatc
     1021 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     1081 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttccgggc
     1141 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     1201 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     1261 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     1321 cgcgattgcc gaaggccagg tgaaagtcga cggcgcggtt gaaacgcgca aacgctgcaa
     1381 aatcgtcgcc gggcagacag tgagttttgc aggtcacagc gtacaggttg ttgcctgatc
     1441 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     1501 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     1561 acgcacgccg ccgttcccct atgcattggt ttagggttgg gttcgaaagt catccctccg
     1621 cgcctgttat ttgccggaat catcctggcg atgctgcccg atgccgacgt cttgtcgttt
     1681 aattttggcg ttgcttacgg caatgttttt ggtcatcgcg ggtttaccca ttcgctggtg
     1741 tttgcgtttg ttgtcccgct gttatgtgtg ctgattggac gtcgatggtt cagggctggg
     1801 ctgattcgct gctggctgtt tttaaccgtc tcgttgctat cgcacagctt gctggattcg
     1861 gtaaccactg gcggtaaagg ggttggctgg ctgtggccgt ggtcagatga acgctttttc
     1921 gctccctggc aggtgattaa agtcgcgccg tttgcattgt ctcgttacac cacagcgtac
     1981 gggcatcagg tgattatttc tgaattgatg tgggtgtggt tgccggggat gctactgatg
     2041 ggaatgttgt ggtggcgcag acgataaccg gatgcgaaaa ctcgcatccg gcaatagcgc
     2101 aattacttac gacgccaggt ggtcccttgc gggccatctt ccagcacgat ccccatctca
     2161 ttaagacggt cacgcgccgc atctgccgcc gcccagtctt tcgctttacg ggcatccaga
     2221 cgctgttgaa ttaacgcttc aatctcagcc acttcgctgt cgtctgcctg cgcgccgctt
     2281 tgcaggaacg cttccggttc ttgctccagc aggcccaata cggcggaaag tttacgcagg
     2341 tgagatgcca ttgcattcgc cgctgccata tcttctactt tcagacggtt tacttcacgc
     2401 gccatatcaa acagcacgga ataggcttcc ggggtgttga aatcgtcgtc catcgcttca
     2461 ataaagcgcg cttcaaacgc ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc
     2521 agcgcagtgt agagacgctc cagcgccgca cgcgcctgct tcaggttctc ttcgctatag
     2581 ttcagctggc tgcgatagtg gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag
     2641 tatttcagca catcgcgcac ggtaaagaag ttacccagcg atttggacat cttctcgcgg
     2701 tcaaccatca ccatccccga gtgcatccag tagttcacat actgaccatc atgggcacag
     2761 gtggactgcg cgatttcgtt ttcgtggtgc gggaacatca ggtctgaacc gccgccgtgg
     2821 atatcaaagt ggttacccag ctgcttgcag ttcattgccg aacattcaat gtgccagcca
     2881 ggacgacccg cgccccacgg agacggccag ctcggttcgc cctctttcga catcttccac
     2941 agaacgaagt ccattgggtt gcgtttgtcg tcgaccacgt caacgcgcgc gcctgcctgc
     3001 agctggtcga gatcctgacg cgacagcacg ccataagttg gatcggtcgg gacgtcgaac
     3061 atcacgtcgc cgttgtccgc cacataagcg tgacctttgg cgatcagttg ttcagtgagt
     3121 tcaataattt ctgcgatatg gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc
     3181 aacgcatcaa aatctttgtg catttcggcg atcatgcgat ccaccagcgc cacaaagctt
     3241 tcgccatttt cattggcgcg tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca
     3301 tacttcagct tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag
     3361 gtacgcccgt gaccgatatg acagagatcg taaacggtga ttccacacac gtacatgccg
     3421 acttccccgg cgtgaatagg cttaaattcc tctttttggc gtgtcagagt attgaagatt
     3481 tttagcatcg aagattccgt ttagacatgt gtgggtaatt gagttgcgta taatacccat
     3541 atttcccgcg cgaatcagca tacattgcgt gatgatccga tcgtgcggtt atgctataac
     3601 accaccctat atatgacccg aactgggttg aagcaccaat caaacggaac aggatgcaaa
     3661 aatggttact ttccacacca atcacggcga tattgtcatc aaaacttttg acgataaagc
     3721 acctgaaaca gttaaaaact tcctggacta ctgccgcgaa ggtttttaca acaacaccat
     3781 tttccaccgt gttatcaacg gctttatgat tcagggcggc ggttttgaac cgggcatgaa
     3841 acaaaaagcc accaaagaac cgatcaaaaa cgaagccaac aacggcctga aaaatacccg
     3901 tggtacgctg gcaatggccc gtactcaggc tccgcactct gcaactgcac agttcttcat
     3961 caacgtggtt gataacgact tcctgaactt ctctggcgaa agcctgcaag gttggggcta
     4021 ctgcgtgttt gctgaagtgg ttgaaggcat ggacgtggta gacaaaatca aaggtgttgc
     4081 aaccggtcgt agcggtatgc accaggacgt gccaaaagaa gacgttatca ttgaaagcgt
     4141 gaccgttagc gagtaatcgt ggcgacactc tttattgcag atcttcatct ctgcgtggaa
     4201 gaaccggcga tcaccgccgg ttttctgcgt tttttagcgg gagaagcccg caaggccgac
     4261 gcgctgtata ttcttggcga tctgtttgaa gcatggattg gcgacgacga tcccaaccca
     4321 ctccatcgcc agatggcggc agcgatcaaa gcggtgtccg attccggcgt tccctgttat
     4381 ttcattcatg gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga aagtggcatg
     4441 acgttattgc cggaagaaaa agtgctcgaa ctttatggtc gtcgggtgct gattatgcat
     4501 ggcgacacgc tgtgcaccga tgacgcgggt tatcaggctt ttcgcaccaa agtccacaaa
     4561 ccctggctgc aaacgctatt cctcgctctg ccgttgtttg tgcgcaaacg cattgccgtg
     4621 cgaatgcgcg cgaacagtaa agaagccaac agcagtaaat cgctggcgat catggacgtt
     4681 aaccaaaacg cggtagtcag tgcgatggaa aaacatcagg tgcaatggct gatccacggg
     4741 catacccatc gcccggcggt gcatgaactt atcgccaatc agcaacctgc ttttcgcgtg
     4801 gtactgggtg cctggcatac ggaaggttcg atggtgaaag tcacggcgga tgacgttgag
     4861 ctgattcatt ttccgtttta aaaaacccgc aactttgctg atttcacagc cacgcaaccg
     4921 ttttccttgc tctctttccg tgctattctc tgtgccctct aaagccgaga gttgtgcacc
     4981 acaggagttt taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg
     5041 gggtccaaaa gcgactgggc taccatgcag ttcgccgccg aaatcttcga aatcctgaat
     5101 gtcccgcacc acgttgaagt ggtttctgct caccgcaccc ccgataaact attcagcttc
     5161 gccgaaagtg ccgaagagaa cggttatcag gtgattattg cgggcgcagg cggcgcagcg
     5221 cacctgccag gcatgattgc cgccaaaacg ctggtgccag tgctgggcgt gccagtacag
     5281 agcgccgcac tgagcggtgt cgatagcctc tactccatcg tacaaatgcc acgcggcatt
     5341 ccggtgggta cgctggcgat tggtaaagct ggcgcggcaa acgcggcatt actggcagca
     5401 caaattcttg cgactcatga taaagaactg caccagcgtc tgaatgactg gcgcaaagca
     5461 caaactgatg aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg
     5521 tcctcggtaa cgggcagtta ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg
     5581 ctgtctggcc ggtcgggctg gacgctgaac cggcggcggt gccttttcaa caaagcgtga
     5641 ttaccgctga gatcgaacgc tggccggaaa cagcattaac ccgcgagctg gcgcgccatc
     5701 cggcctttgt gaaccgcgat gtgttcccga ttattgctga ccgtctgact cagaagcagc
     5761 ttttcgataa gctccacctg ccgactgcac cgtggcagtt acttgccgaa cgcagcgagt
     5821 ggcctgcggt gtttgagcgt ttaggtgagc tggcgattgt taagcgtcgc actggtggct
     5881 atgacggacg cggtcaatgg cgtttacgcg ccgatgaaac cgagcagtta ccggctgaat
     5941 gctacggcga atgtattgtc gagcagggca taaacttctc tggtgaagtg tcgctggttg
     6001 gcgcgcgctg ctttgatggc agcaccgtgt tttatccgct gacgcataac ctgcatcagg
     6061 acggtatttt gcgcaccagc gtcgcttttc cgcaggccaa tgcgcagcag caagcgcaag
     6121 ccgaagagat gctgtcggcg attatgcagg agctgggcta tgtgggcgtg atggcgatgg
     6181 agtgttttgt caccccgcaa ggtctgctga tcaacgaact ggcaccgcgt gtgcataaca
     6241 gcggtcactg gacacaaaac ggtgccagca tcagccagtt tgagctacat ctgcgggcga
     6301 ttaccgatct gccgttaccg caaccagtgg tgaataatcc gtcggtgatg atcaatctga
     6361 ttggtagcga tgtgaattat gactggctga aactgccgct ggtgcatctg cactggtacg
     6421 acaaagaagt ccgtccgggc cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat
     6481 cgcgtctgtc cgcgacgctg gaagccttga tcccgctgct gccgccggaa tatgccagcg
     6541 gcgtgatgtg ggcgcagagt aagttctgtt aacctggtgc tcatttgatt gccggatgcg
     6601 gcgtgaacgc cttatccggc ctacaccgat ccggtaccca ttgtaggcct gataagacgc
     6661 gtcaagcgtc gcatcaggca tggtgcgccg attgccggat gctctcatca ctacagcgaa
     6721 atacaggtcc ccgcttcgcc cgccagcgtc tcttcaattc gcgataacgc cccaatccac
     6781 gcgggtttac cacggcttct gacataacca ctcaccgccg ttaccttcgg ccccatcgaa
     6841 ccatcggctt tggcaaatgg cgctaactca tccggtgtgg catggcgaat ggcacgttgc
     6901 tgcggcattc cccagttttc atataccgca tcagcatcgg tgaggatcac cagtccatcg
     6961 gcgttaattt gctcggcgag caacgcagcg gcgagatctt tatcaatcac tgcttcactc
     7021 cctgctccgt catccgtcac aggcacaccg ccgccgccac tgcaaatcac cacatgcccc
     7081 tctttgagca acaactcgat ggcttcgctg tcgagaattt tacgcggttg cggagacgcc
     7141 accacccggc gcaaatattt accatcacgt ttcatctgcc agccgtaagc cgcttccagt
     7201 gcctcttgtt cttctggctg ataaaccgga ccaataaatt tctctggctg caaaaacgcc
     7261 ggatcatcag gcgaaacttc aatgcgcgtc cgcaccgtcg tgacaggcgg catctgcggc
     7321 tgtgcgctca aactctgcgc cagcatatag ccaatcatcc cctgactttc tgccaccagc
     7381 acatccagcg gatacggttc tacctctttc cacgccagat tctgcaatgc cagcagcccc
     7441 acctgcggcc cgttgccgtg aacaatcgcc agccgataag aacgggccag gcgcgccagc
     7501 gcgggtacag cactggcgat attgcgatat tgattttctg ccgtcagcgc ctcaccgcgc
     7561 tggagtaagg cgttgccccc aagagctaca accagtgttt tcatggtttt ccttgtaata
     7621 attgttggcc aagccagaac cccagcagtg tatctgcccc tgacgtatgc cccagcgcca
     7681 gcagcgaatc aatcgcaaca gcggtacgtt tcgggcaact cagagcatga acaaagtgca
     7741 ggagtggcga ggcgaaatat ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg
     7801 tggtaacgag ttgcagattg tcggaacagg caaaaaacgg acgaccggag cgcgcatcta
     7861 aagcaccata ataccaggcc gccagcagca ttccgctcag cgtgtcatca tgacttggcg
     7921 ttaatccggg gcctttaccc agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg
     7981 cctgaaaaca gtgacgaaac tggcgtaact cagcaggcag cggatcgctt gccgccaacg
     8041 ccagtggccc gaaaagcccg gtttcctccg cgcgttgcat ccatgcagct gcaagtggtt
     8101 gaggatgcgc aggcggcgta atacgcagca aacaataacg ctgtggctgt ttaaccgtga
     8161 aacgcccgag gcgaatccct tgagccacaa cctgtggtcg ttcattgccg cataatccac
     8221 cgcataacgc atcgaattgc gcacggcgaa gcacccatcc tccggggccg aaaccactac
     8281 cctgacgatg cagcgttaac agttcgccgc tttccgtcat caggttaatc gcccgccgcc
     8341 acactcccgc taaccgccag gactggcgat aattcggtgc gctactactg gcaagcagag
     8401 gatggatgat cgtcattacg cgttcctcaa ccaatcccca tgctttcagc cagtgcttcc
     8461 agcgcctgtt caaagcacgc cagcggtgcc cgcacggtgc ctgcgccaat ctgcccgatc
     8521 cccgcctctt tatgggcgat accggtattg atgagtggcg taatgccggt ttctaccacg
     8581 cgacgaatgt ccagtcccag gcacgcgccc tgaaaatccc agcttgggat ctgcaactgc
     8641 atattgcgtt caaggtaaat ttccgccatc tcttcagata ccgccctggc cgcttccatg
     8701 ccactcgcac cgacaaagcg cgttacgcca ggcgctgcga tcattgccgc acctccgata
     8761 ccaaaggttt cggtaatcgc gctatcgccc atatccgggt tcgcctgctc ctgcgagaag
     8821 ccggtgaaaa acagaccttg cggagtgttt acaggcgcag taaaccagcg ttcgcccagc
     8881 ccgcttaccc gaatcccgaa catattgccg ttgcgggtca ttgccgtgac gatgctgcct
     8941 gcgcggatca tcgcgccagc atccatcgcc gccttgcagt aagccatcgc gaggttgagg
     9001 aagaactgat cggtcacgct gaggaaatcc atcacttccg cgatgtgctg tttatcatga
     9061 tcgaggcgag caatttgtgg ggccagcgca cgcattaaca gtgctgagga agcaatattg
     9121 cgttgatgga actcatcgcc catcgtaatg ccctgcgcca tcatcgcagt aagatcgata
     9181 ccgcgctcca tgcgccccag cgccgcgctt aatactggca ttaacacatc gcgcatccag
     9241 cgatggcgag tcaggacatc ttcgccgtaa gcgccaaaac gcatcacttt gccgatacct
     9301 tcgttgaggt tgcagtacgc ccggttgccg tcggtcacgt tctcgaccac cagcatcggc
     9361 atactggcag aagtaatacc gcccattggc ccgacggcat tcacatggtg acaaggaatg
     9421 aagttcactt ccccctgctc cagtattgcc agcgcctgcg cttcatcttt cgcccaacct
     9481 tcgaacagac atgcgcccac gcacgcccct ttcatgggtc cggtcatctc ctgccagcgc
     9541 attggcggcc cggcgtgaag cagtgttttg ccctcgttta gttcgctgat aagtgaagaa
     9601 gccggttgca catccagcca gtgtggacga gcgcgacgaa tttgttcgat aaccgcagca
     9661 ttggcttgcg ccactgatgt aaacatgaga accccttatt gcaaacgttc taataaacga
     9721 gccagttttt tattgccgcc ggcgactggc gaccattggt aatgcacaac cggtttgctg
     9781 gcgctttgta gctccagcgc aaaactgcgt aatccgatgt taatcacggc gacgttttcc
     9841 agtaatgacg gtgtgtgttg ctgtgcggca ggcgagagcg gatgaattaa cgctgccgcc
     9901 agcaaggtgg cttccggtag cgaactcacg accgcaatcc ccgcatcttc cagcgtggcg
     9961 atttgctgcg agcggcattg cgggtcacgt tcagtgcctg tcaccgtggc aatggcatac
    10021 agtggttgat tgtctgaacg cgcagcacag gctttttgcc aggcgcttac cagcgaggcg
    10081 gcaggatcgg cggtcgcacc gaagccaatc acgacatcaa gcagcagcac acgcacttgc
    10141 ggtttagcgc cgagatcggt aattaactgg ttgcgtaagg ttgggtcgat catcggatgg
    10201 ggacgcccga cggtgtagaa atcatcgccg aggtcaataa tctggtggcc atcggcgtcc
    10261 agcatcatgc cgtgatgatg ggcatcatcg gcttccacgc caaggtgtcc ggcaagtaat
    10321 cccgccgctt cggcagccag cgtaccgccg gtatacaaac cgcaaataaa tccgctgctg
    10381 gcaggcatta tcgcgttacg tcgcgccgta acgcgtgaaa gcaggcaagc caggcgtgcg
    10441 gcctcatcca gcgaggaggc aaaccagaca ttctcgtcgc gggccaccgc cggggtataa
    10501 cctaaaaaca gcgccaccgt cggtttgccg gttgctttca tggcattaac aattttcaga
    10561 cgcaccgctt cggcaggtgg ttttgaaaca aatgccagca cttcgctttt ctcgtctgca
    10621 ctgagcattt ccagcgccgt cagcgcactg atgccgccca cttcacggct gaggtcgcgg
    10681 ccgccaaggc caatcgcgtg agtaattccc tcccctgcca gcgcaatctg cgagcacagc
    10741 tcctgaatcc cggtaccgga agcgccaatg acgccaatgt tgccttccgg catcacgtta
    10801 gcaaaagcca gcggcgtgcc ggcaatcatc gacgtaccgc agtccggccc catcaccagc
    10861 aagccttttt cccgcgcgcg ggttttaagt tggatttcat cttccagcgt gacgttatcg
    10921 gagaacatca tcacgttgag gttgcgatcc agcgcctgat tcgccagatt ggcatcgggt
    10981 aatttctgac aggcactgtc ccagcgacgc acctgcgtca acgcctggct gctgcctgac
    11041 ccctgcgcca gttgttttag cgcctcttca agctgctgca taatcgcctg cgcgatcccc
    11101 gcatccgccg cttcgctacg aattgccacg caaatatcgt tcggcgtggc gttattaaaa
    11161 tcgtcatgcc agaaacctgt ggtatctaat aacgctttat tggcgggcgt acccatcatt
    11221 acggaaacat catcaacatt ttctgattcg ctgagttttc gtgaaataat cattaaactg
    11281 accgaatcct gaaaacaccc ttttttaata aaggcgtgga tcataacaac tccttaaaaa
    11341 ttgcacccat ggcaattgca cgagcagtgt taatgtggcg attacggtat cgatgacaac
    11401 gagtaaataa ggtgatgaat atcacacagg gattattaac aaaagaattc aaataaccgc
    11461 catgctatta cgtgttattt tttaata
//
LOCUS       DAZKFZ010000144        11479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000144 DAZKFZ010000000
VERSION     DAZKFZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(84..836)
                     /gene="tcyN"
                     /locus_tag="ACHOQW_003376"
     CDS             complement(84..836)
                     /gene="tcyN"
                     /locus_tag="ACHOQW_003376"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP9574402.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(833..1501)
                     /gene="tcyL"
                     /locus_tag="ACHOQW_003377"
     CDS             complement(833..1501)
                     /gene="tcyL"
                     /locus_tag="ACHOQW_003377"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP9574403.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1516..2502)
                     /gene="dcyD"
                     /locus_tag="ACHOQW_003378"
     CDS             complement(1516..2502)
                     /gene="dcyD"
                     /locus_tag="ACHOQW_003378"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP9574404.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2607..3407)
                     /gene="tcyJ"
                     /locus_tag="ACHOQW_003379"
     CDS             complement(2607..3407)
                     /gene="tcyJ"
                     /locus_tag="ACHOQW_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9574405.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3495..4046)
                     /gene="fliZ"
                     /locus_tag="ACHOQW_003380"
     CDS             complement(3495..4046)
                     /gene="fliZ"
                     /locus_tag="ACHOQW_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP9574406.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRISLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4092..4811)
                     /gene="fliA"
                     /locus_tag="ACHOQW_003381"
     CDS             complement(4092..4811)
                     /gene="fliA"
                     /locus_tag="ACHOQW_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP9574407.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5131..6888)
                     /locus_tag="ACHOQW_003382"
     CDS             complement(5131..6888)
                     /locus_tag="ACHOQW_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFP9574408.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            7154..8551
                     /gene="fliD"
                     /locus_tag="ACHOQW_003383"
     CDS             7154..8551
                     /gene="fliD"
                     /locus_tag="ACHOQW_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP9574409.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            8576..8986
                     /gene="fliS"
                     /locus_tag="ACHOQW_003384"
     CDS             8576..8986
                     /gene="fliS"
                     /locus_tag="ACHOQW_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP9574410.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            8986..9351
                     /gene="fliT"
                     /locus_tag="ACHOQW_003385"
     CDS             8986..9351
                     /gene="fliT"
                     /locus_tag="ACHOQW_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP9574411.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9429..10916
                     /gene="amyA"
                     /locus_tag="ACHOQW_003386"
     CDS             9429..10916
                     /gene="amyA"
                     /locus_tag="ACHOQW_003386"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9574412.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(10950..11363)
                     /gene="yedD"
                     /locus_tag="ACHOQW_003387"
     CDS             complement(10950..11363)
                     /gene="yedD"
                     /locus_tag="ACHOQW_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP9574413.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         2849 a         2969 c         2822 g         2839 t
ORIGIN      
        1 atgctgattt tttaagcagt tttatcaggc ttgaaatggc gttccgcccc gactgtcgaa
       61 tcgtcggggc tgattttttc ttattattgc agcagaaact tctcgaggaa ctggcgggtg
      121 cgaggctgct cgggatcggc aaataacgct tttgcggccc cctgctcgac tatccgcccc
      181 tggtccataa aaatcgcccg gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca
      241 atcaccatcg tgcgcttttc ctgcgccagc tgacggatgg tgttcaggac ttcgcccacc
      301 agctctggat ccagcgctga cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc
      361 agcgcacgcg caatcgcaac acgctgctgt tgaccgccag acaaacgacg tggatagctg
      421 gtttctttac cggccagccc aactttcgcc aacagcttgc gagcgcgcgc cgtggcctct
      481 tctttcggtt cgcctttgac gatcaccggc ccttcaataa tgttctccag caccgtacga
      541 tgcggaaaca aattaaagtt ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc
      601 agcgattttt gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc
      661 gttcccgctt ccggttgttc cagcagattt atgctacgta gcaatgtggt tttgccggaa
      721 ccactcggac cgataattgc caccacttcg ccaggcttta cctcgaggtc gataccgtgc
      781 agcaccgtct gaccgtggaa ttttttcact aggttcttaa cttcaatggc actcatttgg
      841 gttctctctc ctggcgatta agttgattct caaaatggtt ctgcaacgtc gataacacag
      901 tcgccatgat ccagtagatg agcgaagccg ccagatacat ggtgaatacc tccagcgtac
      961 gcgaggtaat caactgcgcc tgacggaaca gctccggcac ctggattgtc gcggccagcg
     1021 acgtatcttt taccagactg ataaagctgt tcgagagcgg cggcaacgcc acccgcgccg
     1081 cctgtggcaa aatcgcgcga cgcatagtct gccatggcgt cataccaata ctggcagctg
     1141 cttcccactg acctttatca atagaagaaa tggcggcgcg aagcgtttcg gcagtatagg
     1201 cggcagtgtt gagcgacaga ccaatcattg ctgacggaat gggatctaat tcgataccaa
     1261 actgcggcag gccgtaatag atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa
     1321 tagagatata aaaacgcgcc agccagcgca cgggccagat tggcgacagg cgcatcagcg
     1381 cgagaataaa ccctagcagt aaaccaaaaa acatgccgcc gatactgagt tgcagcgtat
     1441 acccagcccc tttcagcagg aacggcaagg aatcaataac cagttgtata ctttcttgca
     1501 tgagcgttcc cggagctaaa cgtggggatg ataggcgaac agcgcaggcg cgccgccggt
     1561 atgaataaac agaatcggcc cttcatcttt gaagcgtttc tgactgatac cgtcaatcag
     1621 acccgccatc gcttttccgg tatacacagg atcaagcaga atgccttcca gccgcgccag
     1681 caatttcact gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc
     1741 atcccagagt aaaatttcca ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg
     1801 taggttaacc actttcggca attgatcggc aacggaacgc gacacggtca cgccaatcag
     1861 ttcgctttca ggcatcaggt gttccagccc aacagccagt ccggcgtgag ttccggcact
     1921 gcccgatgcg actaccaccg atgaaatatt aaccgcccct tcacactgtt gcgcgatttc
     1981 cagcgcactc tccacataac ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac
     2041 atacgggcga aagccttgtg cttcgactcg cgtcgccagc tcttccagtt gggcattggg
     2101 atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac agatccagca acaagcgatt
     2161 gccgttggtt aaatagtttt ctgcggttgt gccaatagga ttttccagca gcgccacgca
     2221 gtgcagaccg agtttggcag cgactgcggc agtctgccgc acatggttag actggatcgc
     2281 cccggcagta atcagcgtat cggcaccttc acgcagagca tctgccgcga gaaattctag
     2341 cttacgtaat ttattgccgc ccattgccat gggggtgacg tcatcccgtt tgatgaaaat
     2401 ttcccgtcct agataatcag aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat
     2461 aaactccagc cgtggaaaac gggttaaatt atgcagtggc ataacagcct ccgatgtgtg
     2521 ttgttgtgat tttctcatta tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa
     2581 agcgcccttt tttgtcatta tgctgattat ttggtcacat cagcaccaaa ccatttttcg
     2641 gaaagggctt gcaaagtgcc gtctttttgc atttccgcaa ttgcatcatt cactgctttc
     2701 agcaggtctt catttccttt acgcagcgcc acgccagact cctgacggga gaatgcttca
     2761 ccggttactg ccagcgtatc gttggttttc ttcaccagat ccagcgccgc cagacgatca
     2821 acgaggatcg catcgatacg ccctacgcgc agatcctgat atttggtcgg gtcatcatca
     2881 taggtacgca catcgacgcc ctgaacattc tgccgcagcc actcttcata gttggtgccc
     2941 agaccgaccc ccactttttt gcctttcaga tcatcggctg ttttaatggt gccttcgtta
     3001 ccttttttca ccagcgcctg aataccagaa atggtgtacg gggttgagaa atcgtatttt
     3061 ttcttgcgct catcagaaat ggtgacctga ttaatcacca catcaatacg tttagagtcc
     3121 agcgacgcca gcataccgtc ccatttggtc ggttttagtg acgcctcaac gccaagatgt
     3181 tttgccagct gttgggcaaa ttccacttca aaaccggtta atttgccgtc atccccctga
     3241 aaactgaacg gcggataagt accttccagc cctaccagca gcgtgccgcg ctctttaact
     3301 ttattaagca gaccttcatc tgcaaaactt ttaacgctca tgcccgcaat cagcgctacg
     3361 gccatcacac ccatcaatgc ctgacgtccc agatgtgcta atttcatatt caccccgaat
     3421 gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac aactccgcta
     3481 catcttattc ttatttaata tatatcagaa gcaggcaggc tggaggattt ctgcacaagt
     3541 ccggtacagg tttggcgctg atagtgttga tacttccgta acgcgatgcg gtaattgttg
     3601 gtgctggtgg tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga
     3661 tcgagggaaa tattttgctc gtgtagatga ttccccaaac ggcgcagacg aacgacatat
     3721 tcacgcaccg taccagggct catttcagtt tgctcaaaaa gaaattgctt aaagccgata
     3781 atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc
     3841 gcccatgatt tttgttccgt ttgccagcca ttttcatcaa gcagcgtgtc caggcgggaa
     3901 atctcgattt tattcactat tttgccgtcg cgaactaagc taatgcgatc gagcaattta
     3961 cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg gcttaatggc
     4021 cgtcttttca ggtgctgcac catcattaag aactcctggt agtcaaagtt aaagtgcggc
     4081 atttactgac gttataactt acccagttta gtgcgtaacc gtttaatagc ctggctgtgt
     4141 aactgactga cccgcgattc cccgacctcc agcactgcac caatctcttt gagattcagc
     4201 tcttcctggt aatagagggt taataccagt ttttcgcgct ccggcaacgt ttcgatagct
     4261 tccatcaccc gctggcgcag attactgtcc agtagttgtt gtagcgggtt ttctcgttga
     4321 tgatcatcag taaccaattc gatgctatcg ccgtgctctt cgcgccactc atcgtaggag
     4381 aagagctggc tgttattggt gtcgagcaac atttggcgat aatcggcaat atcgatccct
     4441 aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc cagttgccct
     4501 attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg
     4561 cgaagttcat ccagcatagc gccacggata cgctgcactg cgtaagttgt aaatgccgtt
     4621 ccttgtaggg cgtcatagcg ttcgacggca ttaagtaacc caatgccgcc cgcctgtagc
     4681 agatcgtcaa gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc
     4741 accagcggga cataacgctg ccacagcgag tgtttatcca ttacaccttc agcggtatag
     4801 agtgaattca cgataaacag ccctgcgtta tatgagttat cggcatgatt atccgtttct
     4861 gcagggtttt taatcggacg attagtgggt gaaatgaggg gttatttggg ggttacaggt
     4921 aaattccagg cagaaaaaac cccgccggtg gcggggaagc acgttgctga caaattgcgc
     4981 tttatgttgc cggatgcggc gtaaacgcct tatccggcct acaagaatgt gcaaattcaa
     5041 taaattgcaa ttcaacttgt aggcctgata agcgcagcgc atcaggcaat ttggcgttgc
     5101 cgtcagtctc agttaatcag gttacaacga ttaaccctgc agcagagaca gaacctgctg
     5161 cggtacctgg ttagcttttg ccagcacgga gttaccggcc tgctgaatga tctgcgcttt
     5221 cgacatgttg gacacttcgg tcgcatagtc ggcgtcctga atacgggact gcgcttcgga
     5281 caggttggta gtggtgttgt tcaggttggt gactgcggaa tccagacggt tctggatagc
     5341 acccagggaa gaacggaatt tgtcgatgga gctgattgcg tcgtccaggg cagcaagcgg
     5401 gttggtcgtt gcagaaccag cactggtagt ctcagtagtg attttacccg cggagttcac
     5461 atttacagca gtaccaattg ctggagcata atctttattg gtatcagtcg ctgaagcata
     5521 cccggcaaca gtcacagagc cgttatcctt gttaacgctg taagagactg tatagtcggc
     5581 aacgttagtg ataccacctt tatcatccac ataagacttc gcagcatcac tggattcacc
     5641 cgcggtaaac ccaatagttt tgctcagtac accggaatta aaggtaattg aagatgtcgc
     5701 ggcagccgct ttggtttcgc tcaatactac atctttactc actgtagcct gataagtaat
     5761 cccaccaggg attaacggag ctactggagt ggttgtcgca ggagttgctt ttgcgatggt
     5821 atattctgtg ccattgaatg tcagagaggc accgtcacta ccttcgctcg ctgctttgag
     5881 cagcgctttc atatcagctt tagctgcgct accagcgttg ttagtcgtca agttgcctgc
     5941 atcgtctacg tatgcctggc ttccaccgat ggtgatatta cctgctgaat ccgtttcgaa
     6001 agaaacagta ccatcttttg tggtgtaaga accattaaca gatttaccca catcagcatg
     6061 aagggtggca gctaacgcat cacgttttga agctgaatca gcagcctgcg ccgcggcgtc
     6121 tacacccgta ccagcagtag atttagtggt agtaaaatca ccagatttag cgttgtaagt
     6181 ataatctacg ccgccaactg tgactttatc gccattccct aatttatcga atgcagcatc
     6241 ggtagttaac aaggtatttt cggttttcag atcataaaga cctgtcgtgg tgtttaactt
     6301 cgcgccagca gaagttaaat cacttaccgt tgcagctttg ttggtaatag tacctttacc
     6361 atttacgtta aagccattca gacccagagt atcagaatcg atttttttca ggtcgatcgt
     6421 gatggtttcg ccgtcattcg caccaacctg aattttcatt gaaccgtctt tcgccagcac
     6481 gttcacgccg ttgaactggg tctggccgga tacgcggtca atttcatcaa gacgagattt
     6541 gatttcgtcc tggatggagt ccaggtcaga atcggagtta gtccctgtag tggcctgaac
     6601 cgtcagttca cgaatacgct gtaagttgtt gttgatttcg gacagcgcgc cttcggtggt
     6661 ctgcgcaaca gaaataccgt cgttggcgtt acgggccgcc tgagtcaggc ctttaatgtt
     6721 agaagtaaaa cggttagcaa tcgcctgacc tgcggcgtca tccttcgcgc tgttaatacg
     6781 caagccagaa gacagacgct cgatagaact cgacagcgca gactggttct tgttgatatt
     6841 attttgagtg atcagcgaga ggctgttggt attaatgact tgtgccatga ttcgttatcc
     6901 tatattgcaa gtcgttgatt acgtattggg tttccacccg tcggctcaat cgccgtcaaa
     6961 cctgttatcg tctgtcgtaa aacaaccttt agaatttttt tcacaaacag ccattttttg
     7021 ttagtcgccg aaatactctt ttctctgccc cttattcccg ctattaaaaa aaacaattaa
     7081 acgtaaactt tgcgcaattc aggccgataa ccccggtatt cgttttacgt gtcgaatgat
     7141 aaaaggaaat cgcatggcaa gtatttcatc gctgggagtc gggtcaggtc tggatttaag
     7201 ttcaatcctt gatagcctca ccgccgcgca aaaagcgacg ctaaccccca tttcaaatca
     7261 gcaatcgtcg tttaccgcta aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac
     7321 tttccagacc gccaatactg cattgtctaa agccgatctt ttttccgcca ccagcagcac
     7381 caccgcgttc agtgccacca ccgcgggtaa cgccatcgcc gggaaataca ccatcagcgt
     7441 cacccatctg gcgcaggcgc aaaccctgac cacgcgcacc accagagacg atacgaaaac
     7501 ggcgatcgcc accagcgaca gtaaactcac cattcaacaa ggcggcgaca aagatccgat
     7561 taccattgat atcagcgcgg ctaactcgtc gttaagcggg atccgtgatg ccatcaacaa
     7621 cgcaaaagca ggtgttagcg cgagcattat taacgtgggt aacggtgatt atcgtctgtc
     7681 agtcacatca aatgacaccg gccttgacaa tgcgatgacg ctctcggtta gcggtgatga
     7741 tgcgctacaa agttttatgg gctatgacgc cagtgccagc agcaacggca tggaggtctc
     7801 ggtcgccgcc cagaatgcgc agctgacggt caacaacgtc gccattgaga acagcagcaa
     7861 caccatcagc gacgcgctgg aaaacatcac cctgaacctg aacgatgtca ccacgggcaa
     7921 ccagacgcta accatcactc aggacacctc caaagcgcaa acggcgatta aagattgggt
     7981 gaatgcctat aactcgctaa tagatacctt cagcagcctg accaaataca ccgccgtaga
     8041 tgcgggagct gatagccaga gttctagcaa tggcgcactg ctcggcgact ccacgctgcg
     8101 gacgattcag acgcagttga agtcgatgct gagtaatacc gtcagttctt ccagctataa
     8161 aacgttggcg cagattggta tcacgaccga tcccagcgat ggcaaactgg aactggatgc
     8221 cgacaaactc accgctgcac tgaaaaaaga tgccagcggc gtaggtgcat tgattgttgg
     8281 cgatggtaaa aaaaccggca tcacgaccac catcggcagc aacctgacca gttggctttc
     8341 gacaacgggc attattaaag ccgctaccga tggcgttagt aagaccctga ataaattaac
     8401 taaagactac aacgccgcca gcgatcgcat tgacgcgcag gtcgcgcgct acaaagaaca
     8461 atttacccaa ctggacgttt taatgacctc gttaaacagc acaagcagct acttaacgca
     8521 gcagttcgaa aacaacagta attccaagta atcattattc atcgggagac aggtcatgta
     8581 cgcggcaaaa ggcacccaag cctatgcaca aattggcgtc gaaagcgccg taatgagcgc
     8641 cagccagcag cagctggtca ccatgctatt tgatggagtg ctgagcgcac tggttagagc
     8701 gcgcctgttt atgcaggaca acaatcagca aggcaaaggc gtctctttgt caaaagcgat
     8761 caacatcatt gagaacggac tgcgggtgag tcttgatgaa gagagcaaag acgaactaac
     8821 ccaaaacttg attgctcttt atagctatat ggtcaggcgt ttgctgcaag ccaatttacg
     8881 caacgatgtc tccgcagtcg aagaagtgga aacattaatg cgcaatattg ccgatgcctg
     8941 gaaagagtcg ttactctccc cttctttgat tcaggaccca gtctgatgaa ccatgcaccg
     9001 catttatatt tcgcctggca acaactcgtc gaaaaaagcc agctcatgtt acgcctggca
     9061 acggaagaac aatgggacga actcatcgcc agcgaaatgg cgtatgttaa tgcggtgcag
     9121 gagattgcac atttgactga agaggttgac ccgtccacca cgatgcagga gcagctccgc
     9181 ccgatgctgc gcctgattct cgacaacgaa agcaaggtaa agcagttatt acagattcgg
     9241 atggatgaac tggcgaaact ggtcggtcag tcatcggtgc aaaaatcggt gttgagtgcc
     9301 tatggcgatc agggcggctt tgtgctggct ccgcaggata acctcttttg aatctgaatg
     9361 agtcgatggc tcgcgaataa tccgattacg gctacgcttc taatgttccc cttgaatgga
     9421 gtcgaagaat gcgtaatccc acgctgttac aatgttttca ctggtattac ccggaaggcg
     9481 gtaagctctg gcctgaattg gccgagcgcg ccgacggttt taatgatatt ggtatcaata
     9541 tggtctggtt gccgcccgcc tataaaggcg catcgggcgg gtattcggtc ggctacgact
     9601 cctatgattt atttgattta ggtgagtttg atcagaaagg cagcatccct actaaatatg
     9661 gcgataaagt acaactgctg gccgccattg acgccctgaa acgtaatgac attgcggtgc
     9721 tgttggacgt ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc
     9781 agcgcgttaa tgctgatgac cgtacgcaaa ttgatgaaga aatcattgaa tgcgaaggct
     9841 ggacgcgtta caccttcccc gctcgtgccg ggcaatactc acagtttatc tgggatttca
     9901 aatgttttag cggtatcgac catatcgaaa accccaatga agatggcatt tttaaaattg
     9961 ttaacgacta caccggcgaa ggctggaacg atcaggttga tgatgaactc ggtaatttcg
    10021 attatctgat gggcgagaat atcgattttc gcaatcatgc cgtgacggaa gagattaaat
    10081 actgggcgcg ctgggtgatg gaacaaacgc aatgcgacgg ttttcgtctt gatgcggtca
    10141 aacatattcc agcctggttt tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa
    10201 agccgctgtt tattgtggcg gagtactggt cgcatgaagt tgataagctg caaacgtata
    10261 ttgatcaggt ggaaggcaaa accatgctgt ttgatgcgcc gctgcagatg aaattccatg
    10321 aagcatcgcg catgggacgc aactacgaca tgacgcagat tttcactggg acgctggtgg
    10381 aagccgatcc ttttcacgcc gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag
    10441 ccctcgaagc accggtcgaa ccgtggttta aaccgctggc gtatgctcta attttgttgc
    10501 gggaaaatgg cgttccttcg gtgttctatc cggacctcta cggtgcgcat tacgaagatg
    10561 tcggtggtga cgggcaaacc tatccgatag atatgccaat aatcgaacag cttgatgagt
    10621 taattctcgc ccgtcagcgt ttcgcccacg gtgtacagac gttatttttc gaccatccga
    10681 actgcattgc ctttagccgc agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt
    10741 cgaacggaga tgatggcgaa aaaaccattc atttaggaga gaattacggc aataaaacct
    10801 ggcgtgattt tcttgggaac cggcaagaga gtgtagtaac cgacgaaaac ggcgaagcga
    10861 ccttcttttg caacggcggc agcgttagcg tgtgggttat cgaagaggtg ctttaaattc
    10921 atccccggcg gcaagccggg gagatttcat tacggcaatg gcgtcggtaa cggcgcttta
    10981 tccagcgcag cggcacactc tgccgtcggg cgatccacac gctccatcgt cataccatcg
    11041 tactcaatgg tgttaccgtc acgctcaacc tcgtacagct cacgttttac ggtaacgtta
    11101 gtgagatcat cagacatcaa tgtcaacttg cccggcacag caatcacccg ctgccactga
    11161 cggcaatcta gggtatcgcc ctctttagtg acaatcaggc tgcctatcgc ttccgggctg
    11221 accagcgcac gttgcggccc tttggtctgc cagtagcccg ccagccagtc cggcgcgggg
    11281 gttttcacga cattgttata gttttcgact tcggcacagc ccgccagcaa catgagcgcg
    11341 cctgcaattg cgagtttttt catcattcgt cctgcatgag aagaaaagag gattgtggca
    11401 ttaaagccct gatgtcgcca gcctttccag gttcagagag aaatttgatc taactattat
    11461 ttttgtgaat tattacccc
//
LOCUS       DAZKFZ010000145        11324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000145 DAZKFZ010000000
VERSION     DAZKFZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            231..1619
                     /gene="narU"
                     /locus_tag="ACHOQW_003388"
     CDS             231..1619
                     /gene="narU"
                     /locus_tag="ACHOQW_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP9574414.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1701..5441
                     /gene="narZ"
                     /locus_tag="ACHOQW_003389"
     CDS             1701..5441
                     /gene="narZ"
                     /locus_tag="ACHOQW_003389"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP9574415.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5438..6982
                     /gene="narH"
                     /locus_tag="ACHOQW_003390"
     CDS             5438..6982
                     /gene="narH"
                     /locus_tag="ACHOQW_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9574416.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6982..7677
                     /gene="narW"
                     /locus_tag="ACHOQW_003391"
     CDS             6982..7677
                     /gene="narW"
                     /locus_tag="ACHOQW_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9574417.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7674..8354
                     /gene="narI"
                     /locus_tag="ACHOQW_003392"
     CDS             7674..8354
                     /gene="narI"
                     /locus_tag="ACHOQW_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9574418.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8433..9326
                     /gene="yddE"
                     /locus_tag="ACHOQW_003393"
     CDS             8433..9326
                     /gene="yddE"
                     /locus_tag="ACHOQW_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP9574419.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9422..10266)
                     /gene="nhoA"
                     /locus_tag="ACHOQW_003394"
                     /pseudo
     CDS             complement(9422..10266)
                     /gene="nhoA"
                     /locus_tag="ACHOQW_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10565..11008
                     /locus_tag="ACHOQW_003395"
     CDS             10565..11008
                     /locus_tag="ACHOQW_003395"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9574420.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11012..11239)
                     /gene="pptA"
                     /locus_tag="ACHOQW_003396"
     CDS             complement(11012..11239)
                     /gene="pptA"
                     /locus_tag="ACHOQW_003396"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFP9574421.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2663 a         2948 c         2992 g         2721 t
ORIGIN      
        1 catgacatgc gtacatagct taaccccgtt taatggttag cttgttagtt acatttagta
       61 acacataact ggctgcttaa tccccattct gcccccgcgg ttacgctttc ttatctattc
      121 tttcaatcag cacttcaccc atttcatcct taattctaat aactggctat tgcgccttac
      181 ctcctgtttt gtcttttttt cattaccaat gtgtacatgt gaggaacaat atggcactgc
      241 aaaatgagaa aaatagtcgt tatcttttac gcgactggaa accagaaaat ccggccttct
      301 ggaaaaataa aggaaaacat attgctcgaa gaaacctctg gatatcagtc agttgtctac
      361 ttcttgcctt ctgtgtctgg atgctattta gcgcagttac cgttaatctc aataaaatcg
      421 gttttaattt cactaccgat caactctttt tattaaccgc attaccctcc gtttctggcg
      481 cattattacg tgtcccctac tcctttatgg tgcctatatt cggtggacgt cgatggacgg
      541 tttttagtac tgcaatcctg attattcctt gcgtctggct cggaattgcc gtgcagaatc
      601 cgaatactcc ttttgggata tttatcgtta tcgctttgct atgcggtttt gcaggtgcga
      661 actttgcttc gagcatgggc aatatcagtt tcttctttcc aaaagccagg caaggtagcg
      721 ctcttgggat taatggcgga ttaggaaact taggtgtaag tgtaatgcag ctggttgcac
      781 cgctggtcat ttttgtaccc gtatttgcct ttctcggcgt caatggagta ccgcaggccg
      841 acggttcggt gatgtcgctg gcgaatgccg catggatttg ggtaccgcta ctagcgattg
      901 ccacgatcgc cgcctggtca gggatgaatg atatcgccag ttcacgcgcg tcaattgccg
      961 accagctccc tgtcttacaa cgcctgcatc tctggctgct gagcctgctt taccttgcca
     1021 ccttcggttc gtttatcggt ttttctgcgg gtttcgccat gctggcaaaa acccagttcc
     1081 cggatgtgaa tattctgcgc ctggcgttct ttggcccatt tatcggtgcc atcgcgcggt
     1141 cggtgggtgg tgctatttcc gataaattcg gcggcgtgcg ggtgacgttg atcaacttta
     1201 tttttatggc gattttcagc gccctgctgt tccttacctt accgggcaca ggctccggta
     1261 acttcatcgc cttttacgcc gtatttatgg ggctgtttct gaccgcgggt ctgggaagtg
     1321 gttctacttt ccagatgatc gccgtcatct ttcgccagat aaccatttat cggatgaaga
     1381 tgaaaggcgg tagtgatgag caagctcaga gagaagccgt caccgaaacg gcggcggctc
     1441 tgggctttat ctcagccatc ggcgcagtgg gcggcttttt tattccgcag gcatttggca
     1501 tgtcactcaa tatgaccggc tctccagtgg gcgcgatgaa agtgttttta atcttctaca
     1561 tcgtttgtgt gctactgacc tggctggttt atggtcggcg gaagttcagc caaaaataag
     1621 agatcgatgc ctgatgtaca accgtgtcgc atccgacaag gcatcagatc ccaattcatt
     1681 cctggagcag gagtcatgtc atgagtaaac ttttggatcg ctttcgctac ttcaaacaaa
     1741 agggcgatac ctttgccgat ggtcacggac aggtgatgca tagcaaccgc gactgggagg
     1801 acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg ttccacccac ggtgttaact
     1861 gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg tctggtgacc tgggaaatcc
     1921 aacagaccga ctacccgcgc actcgccctg acctgcccaa tcatgaacct cgcggctgcc
     1981 cgcgtggcgc aagttactcc tggtatcttt acagcgctaa ccgcctgaaa tacccgctca
     2041 ttcgtaaacg actgattgaa ctgtggcgcg aagccctcaa acaacacagc gatccggtac
     2101 tggcgtgggc atcgattatg aacgatccgc aaaagagcct gagctacaaa caagtgcgtg
     2161 ggcgcggcgg gtttatccgc tccaactggc cggaactaaa ccagctgatt gccgccgcta
     2221 acgtctggac tatcaaaacc tacggcccgg atcgcgttgc cggtttctcg ccgatcccgg
     2281 cgatgtcgat ggtttcttac gccgccggaa cacgttatct gtcactatta ggtggcacct
     2341 gtttaagctt ctacgactgg tattgcgacc tgccgcccgc ctcgccgatg acctggggcg
     2401 agcaaaccga cgtaccggaa tctgccgact ggtataactc cagttacatc atcgcgtggg
     2461 ggtctaacgt accgcagaca cgtacgccgg acgcccattt ctttaccgaa gtacgctaca
     2521 aaggcactaa aaccatcgcc attacccctg actactctga agtggccaaa ttgtgcgacc
     2581 agtggctggc accaaaacaa ggcactgata gcgccctggc gatggcaatg ggccatgtga
     2641 ttttaaaaga gtttcatctc gataatccca gcgactactt tatcaactac tgccgccgct
     2701 atagcgacat gccgatgctg gtaatgctgg aacctcgcga cgatggtagc tacgtacccg
     2761 ggcgcatgat ccgcgcgtct gacctggtag atggactagg cgaaagcaat aatccgcagt
     2821 ggaaaaccgt ggcggttaat accgcaggtg aattggtagt gccgaatggt tcgattggtt
     2881 tccgctgggg agaaaaaggc aaatggaatc tggaatccat tgccgccggt aaggaaaccg
     2941 aattgtcgtt aaccctgctc ggtcaacatg acgccgttgc aggcgtggct ttcccctact
     3001 ttggcggcat cgaaaatccg cattttcgca gcgtcaaaca caatcctgtc ctggtgcgtc
     3061 aattacccgt taaaaacctg acattagccg gtggcagcac ctgtccagtg gtcagcgttt
     3121 atgatttggt actggcgaat tacggcctcg atcgcgggct ggaagatgaa aacagcgcga
     3181 aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg tgagcaaatt accggcgtgc
     3241 cgcgccagta tattgaaacc atcgctcgtg aatttgccga tactgcccat aaaacgcatg
     3301 ggcgctcgat gattatcctc ggcgcaggtg ttaaccactg gtatcacatg gacatgaact
     3361 accggggaat gatcaatatg ctgatcttct gcggttgtgt cggacaaagc ggtggcggct
     3421 gggcacacta tgtcggtcag gaaaaactgc gcccgcaaac cggctggttg ccgctggcct
     3481 ttgcgctcga ctggaaccga ccaccgcgcc agatgaacag cacctcgttt ttctacaatc
     3541 attccagcca atggcgctat gaaaaagtct ctgcgcagga gttactttca ccgcttgccg
     3601 atgccagtaa gtacagcggt catctgattg attttaacgt tcgcgccgaa cgcatgggct
     3661 ggctaccttc tgcgccgcag ttggggcgta acccgctcgg gattaaagct gaagccgaca
     3721 aggccggatt atcccccaca gaatttaccg cccaggcgct gaaatcgggc gatttacgta
     3781 tggcctgcga acaaccagat agcggcagca atcatccgcg taatttgttt gtctggcgtt
     3841 ctaacctgct tggctcctcc ggcaaaggcc acgagtatat gcagaagtat ctgctgggga
     3901 ccgaaagcgg gattcagggc gaggaactcg gtgccagcga cgggatcaaa ccggaagaag
     3961 tcgagtggca aactgcggcg attgaaggca agctcgacct gctggtgacg ctcgacttcc
     4021 gcatgtccag tacctgcctg ttctccgata tcgttctgcc caccgccacc tggtatgaaa
     4081 aagacgatat gaacacctcg gatatgcatc catttattca tccgctttct gcggcggtcg
     4141 atccggcctg ggaatcacgc agcgactggg aaatctacaa aggtattgcc aaagcatttt
     4201 cgcaagtgtg cgtgggccat cttggcaaag aaaccgacgt ggtattacaa ccactgctgc
     4261 atgactctcc ggcagagctc tcacagccgt gtgaagtgct cgactggcgc aaaggcgaat
     4321 gcgatctgag cccgggtaaa accgcgccga atattgtggc ggtggagcgc gactaccctg
     4381 ctacgtatga acgctttacc tcgctcgggc cattgatgga caaacttggc aacggcggta
     4441 aagggatttc gtggaatacg caggatgaaa tcgatttcct cggtaaactc aattacacca
     4501 agcgtgatgg cccagcgcag gggcgtccgc tgattgacac cgccattgac gcttcagaag
     4561 tgattctggc actggcacca gaaaccaacg gtcatgttgc agttaaagcg tggcaggcgc
     4621 tgggcgagat caccggacgc gaacataccc atctggcgct gcacaaagag gacgagaaga
     4681 ttcgctttcg cgatattcag gcgcagccgc gtaaaattat ctccagcccc acatggtctg
     4741 gtctggaaag cgatcacgtc tcctataatg cgggatacac caacgttcat gagttaattc
     4801 cgtggcgcac gctgtcggga cgccagcagc tctatcagga tcatccgtgg atgcgtgctt
     4861 ttggtgaaag cctggtggca tatcgcccgc ctatcgacac ccgtagcgtc agtgagatgc
     4921 gccagatccc gccaaacggc ttcccggaaa aagcacttaa cttcctgacg ccgcaccaga
     4981 aatggggcat tcactcaacc tacagtgaaa acctgctaat gctgacgctc tctcgcggtg
     5041 gaccgattgt ctggatcagc gaaaccgatg cccgtgaact aaccattgtc gataacgact
     5101 gggtggaagt gtttaacgcc aatggcgcgc tgacggcccg cgcggtggtc agccaacgtg
     5161 taccgccggg tatgaccatg atgtatcacg ctcaggaacg cattatgaat attcctggtt
     5221 cggaagtaac tggcatgcgc ggcggtattc ataactcggt cacccgcatt tgcccgaaac
     5281 caacgcatat gattggcggt tacgcgcagc tggcctgggg ctttaactac tacggcaccg
     5341 tcggctcgaa ccgcgacgaa ttcatcatga tccgcaagat gaagaacgtt aactggctgg
     5401 atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg aaaatccgtt cgcaagtcgg
     5461 catggtgctt aatctcgata aatgtatcgg ctgccatacc tgttcggtga cctgtaaaaa
     5521 cgtctggacc ggacgcgaag gcatggagta cgcatggttt aacaacgtcg aaaccaaacc
     5581 gggcattggt tatccgaaaa actgggaaga tcaggaagag tggcaaggcg gctgggtccg
     5641 cgatgtgaat ggcaagatac gcccgcgtct gggcagcaag atgggtgtaa taaccaaaat
     5701 cttcgccaac ccggtggtgc cgcagattga tgattactac gaacctttca ccttcgacta
     5761 cgaacatttg catagcgcac cggaaggcaa acatatccct actgctcgcc cgcgttcgct
     5821 gattgacggt aagcggatgg acaaagtgat ctgggggcca aactgggaag aactgctggg
     5881 cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag gccatgcaaa aggagatgta
     5941 cgggcagttt gaaaacacct tcatgatgta cctgccgcgc ctgtgcgaac actgcctcaa
     6001 tcccagctgc gtggcgacct gcccaagcgg cgctatctac aaacgcgaag aagacggcat
     6061 tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg tgcattagcg gttgtccgta
     6121 caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa aaatgtatct tctgttaccc
     6181 acgaattgag tccggtcaac cgaccgtgtg ctcagaaacc tgcgtgggtc gcatccgata
     6241 tctgggcgtg ctgctttacg acgctgaccg catcgaggaa gcggcgagca ccgagcgcga
     6301 ggttgacctc tatgaacgcc agtgcgaagt gttcctcgat ccgcacgatc cctcagtgat
     6361 cgaggaagcc ctgaaacaag gtattccaca aaatgtgatt gacgctgccc agcgttcgcc
     6421 agtctacaaa atggcgatgg actggaaact ggcactaccg ctgcaccctg aataccgcac
     6481 cctgccgatg gtctggtacg ttcctccgct gtcaccgatt cagtcctacg cagatgcggg
     6541 cggtttgccg aaaagcgaag gcgtgctgcc cgccatcgaa agcctgcgta ttccggtgca
     6601 atatctcgcc aatatgttga gtgccggcga taccggtccg gtactgcggg cgctgaaacg
     6661 gatgatggcg atgcgccact atatgcgttc acaaaccgtg gaaggcgtta ctgatactcg
     6721 tgccatcgac gaagtaggcc tgagcatcgc ccaggtcgaa gaaatgtatc gctatctcgc
     6781 cattgccaac tatgaagatc gttttgttat cccgaccagc catcgggaaa tggcgggcga
     6841 tgccttcgca gaacgcaatg gctgcggttt tacctttggt gacggttgtc acggctcgga
     6901 cagcaaattc aacctgttca acagtagccg tatcgatgcc atcaacatca ccgaagtgcg
     6961 cgacaaagcg gagggcgaat aatgcagatc ctcaaagtga tcggcctgtt gatggagtat
     7021 ccggacgaga tgttgtggga atgcaaggag gacgcgctgg cgttgatccg ccgcgatgcg
     7081 ccgatgctta cggatttcac ccgcaacctg cttaacgcgc cgctgctgga taaacaggcc
     7141 gaatggtgcg aagtgtttga ccgcgggcgc accacgtcgc tgctgctgtt cgaacatgtt
     7201 catgccgagt cccgcgatcg cggccaggca atggtggacc tgctggcgga gtatgaaaag
     7261 gtcggcctgc agctggattg tcgggaactg cccgattatc taccgctgta tctggagtat
     7321 ttaagcgtac tgcctgacga tcaggcgaaa gaaggattgc tcaacgttgc gccgatcctc
     7381 gccctgcttg gcggtcgctt aaaacaacgc gaggcaccgt ggtacgcgtt gtttgatgct
     7441 ctgctgcaac tggcgggaag tacactttca agtgacagtg tcacaaaaca agtaaacagc
     7501 gaagagcgcg atgacacccg ccaggcgctt gatgcagtgt gggaagagga acaggtgaag
     7561 tttattgaag ataacgccac ggcgtgtgac agctcgccgc ttaatcaata tcagcgacgc
     7621 tttagtcagg atgtcgcgcc gcaatatgtc gacatcagtg cgggaggtgg gaaatgattc
     7681 agtatctgaa cgtctttttt tacgatatct acccgtacat ttgtgcgacg gtgtttttcc
     7741 tcggcagctg gctgcgctac gactacgggc agtacacctg gcgcgcctcc tcaagtcaga
     7801 tgctcgataa acgcgggatg gtgatatggt cgaatctgtt ccatatcggc attttgggga
     7861 tcttcttcgg gcacctgttc ggcatgttaa cgccacactg gatgtacgcg tggtttttgc
     7921 cagtggcagc gaaacaacta atggcgatgg tgctcggtgg tatttgcggt gtgttgacgt
     7981 tgattggcgg cgcaggactg ctgtggcgca ggctgacaaa tcagcgcgtg cgtgccactt
     8041 ccactacgcc ggatatcatc atcatgagca ttttgctgat ccagtgtctg ttggggctaa
     8101 gtaccatacc gttctcagca cagtatcctg atggtagcga aatgatgaag ctggtgggtt
     8161 gggcgcaaag cattgtcact ttccgtggtg gatcgtcaga gatgcttaac ggtgtagcgt
     8221 tcgtcttccg cctgcatctg gtgttgggaa tgaccatctt cttgctcttc ccattcaccc
     8281 gactggtgca tgtgtggagc gccccgtttg agtactttac tcgtcggtat cagattgtgc
     8341 gctcgcggcg ataatttcac cctggccgga tagcccttcg ctctccggcc acttgcaggc
     8401 tatgatagtt ctccagtcac aggagaacgt taatgaaacc gcaggtttac cacgtcgatg
     8461 cttttacctc tcaaccattt cgcggcaatt ctgccggtgt tgttttcccc gccgataatc
     8521 ttagcgaagc gcaaatgcag cttatcgccc gcgaattagg tcattcggaa accgcttttc
     8581 tgctgcacag cgacgacagc gatgtgcgca tccgctactt tacgccaaca gttgaagtgc
     8641 ctatttgcgg tcacgcgaca gtagctgcgc actatgtacg tgcgaaggtg ttaggtttag
     8701 gaaattgcac ggtctggcaa acatcgctgg caggaaaaca tcgcgtgact atcgaaaagc
     8761 accacgatgg ttatcgtatt tcgctggaac aaggtacgcc aggctttgag ccaccgctgg
     8821 aaggtgaaac acgtgcggcg attatcaacg cgcttcatct cactgaggac gatattctgc
     8881 caggcttgcc aattcaggtg gcaaccacag gacactcaaa agtgatgatc ccactgaaac
     8941 cggaagtgga tatcgacgcc ctttcgcctg accttaatgc gctgaccgct atcagcaaac
     9001 agattggctg caatggtttc ttcccgttcc agatccgccc aggcaaaaac gaaaccgatg
     9061 gtcgcatgtt ctcgcctgcg attggtattg tggaagatcc ggtgactggc aatgccaatg
     9121 gcccgatggg tgcatggttg gtacatcaca acgtattgcc ccacgatggc aacgtgttgc
     9181 gtgttaaagg ccatcagggg cgcgcattgg ggcgtgacgg tatgattgaa gtgacggtga
     9241 caattcgtga taaccaaccg gaaaaagtca ccatttctgg cgcggcggtg attttgtttc
     9301 atgccgaatg ggcaattaaa ctctgaaacc gttgcctgat gcgcttcgcc tgtcaggtct
     9361 acaaccgggc taatgtatgc cggataaggc gtttacgccg catccggcaa cctgacataa
     9421 attattttcc cgcctccggg tgcgtatcaa acgccgccat caccagcgct aactcatcca
     9481 cggtaaagcc atgtttcgca tcatccacgc ccagaccaaa ctgttcttgc atcacagcat
     9541 ataacgacgc cacatccggt agatttcgct gctccaccgc gtgcccattt tcataatggg
     9601 aaaatgaaaa ttggtcagtg tcagcttgcc gccgtccggc aaatggcggc acatcagcaa
     9661 atgatggcga aaatgcgact gcggccagtg cgccgaccag aaattaccca tcacataatc
     9721 gctttgttgc tgctcgcaga gatcaaaacg gtacatcgac tgccagtgct gatgatgatt
     9781 aaactgcaac acccaatcat caccctcctg caacaaccga tactctccgt gtggcgtggt
     9841 ctgcacaaga tcgggaacta aacgaatcgg cgcggttagc gtctgcccac cgaaaccgac
     9901 atcagcaatc catttttcct cttccagttc caccaacagc aaacgatggg tgcgcggcgg
     9961 taatgctggc ggatttgata acactacccg ccctaacaag ctgcgaacgt taaaccccag
    10021 ctcgcgtaac acccgctcaa aaacgccatt ctgctcaaaa cagtaaccgc cacgacgggc
    10081 tatcaccagt ttctcttcca gcgattgatc atcaagctgc atttccctcg gcagcaaaac
    10141 gtcgaggttt tcaaacggaa tggtgcaatt gtgtttcagg tgcaatgcac gaagcgtatc
    10201 aatattgacc gcagcagctc ccgaccagtt aatacgggca aaatagtgat tcagaatggg
    10261 cgtcatcagg cgtttccctt agtggttttc tcaacttata acctggaata gacgccgcac
    10321 cggctatttt gtgcgatcgc ggacatgcat cacgttcgca aatcgccaat cccgttaatt
    10381 tcgttactta ttgcgttacg gactacgctt aaaaaaacct tcttcgcgag gcgataccat
    10441 gagccgattc atccccatag aattacacca tgccagccgg ctgttaaatc atggcccaac
    10501 tgtcatgatc accagtttcg atgagcaatc ccagcggcgt aacattatgg ctgcagcctg
    10561 atcaatgccg gtggagtttg aacctccacg cgtgacgatt gtggtagata aatcgacatg
    10621 gaccagggag ttgattgaac gtaacggtaa atttggcatc gttatcccgg gcgttgcagc
    10681 aactaactgg acgtgggcag tgggaagtgt gtcgggtcgt gaagaagata aatttaattg
    10741 ctatggcatt ccggttgtga gaggcccggt atttggtttg cctctggtcg aggaaaaatg
    10801 tctggcgtgg atggagtgtc gattgctacc tgcgacttct gcgcaagaag aatacgacac
    10861 gctgtttggc gaagtagtat cagcagcggc agacgcacgg gtatttgtcg aaggccgctg
    10921 gcagtttgat gatgataagc tcaatacgtt gcatcattta ggtgctggga cgtttgttac
    10981 cagcggcaag cgtgttacgg cgggttaagc attacatgct gtaaccgggt ttctttatca
    11041 aagcctccat ttggggcgcg atttcggcat cccagatagc ttgccaagat tctggttgaa
    11101 tctgttgtag agcaatgctt atcgaactgt ctttactgtt cagatggcga ataataacgt
    11161 cggtaatatc tgcagcaagt gctgcttttt gttgttcgtc cagttcacgc ggaaaacatt
    11221 taatgtctat gtgcggcatg attacgctcc ttttgaaaga tgtcagtaaa attaacacac
    11281 attgtgaatg tcgtgtgtcc ggtaaagcgt ttttacgaca ataa
//
LOCUS       DAZKFZ010000146        11302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000146 DAZKFZ010000000
VERSION     DAZKFZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..126
                     /locus_tag="ACHOQW_003397"
     CDS             <1..126
                     /locus_tag="ACHOQW_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9574422.1"
                     /translation="YLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            105..209
                     /gene="speFL"
                     /locus_tag="ACHOQW_003398"
     CDS             105..209
                     /gene="speFL"
                     /locus_tag="ACHOQW_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP9574423.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            520..2718
                     /gene="speF"
                     /locus_tag="ACHOQW_003399"
     CDS             520..2718
                     /gene="speF"
                     /locus_tag="ACHOQW_003399"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP9574424.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2715..4034
                     /gene="potE"
                     /locus_tag="ACHOQW_003400"
     CDS             2715..4034
                     /gene="potE"
                     /locus_tag="ACHOQW_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP9574425.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4091..5731)
                     /gene="pgm"
                     /locus_tag="ACHOQW_003401"
     CDS             complement(4091..5731)
                     /gene="pgm"
                     /locus_tag="ACHOQW_003401"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP9574426.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5757..6302)
                     /gene="seqA"
                     /locus_tag="ACHOQW_003402"
     CDS             complement(5757..6302)
                     /gene="seqA"
                     /locus_tag="ACHOQW_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP9574427.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6487..7251
                     /gene="ybfF"
                     /locus_tag="ACHOQW_003403"
     CDS             6487..7251
                     /gene="ybfF"
                     /locus_tag="ACHOQW_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9574428.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7391..7684
                     /gene="ybfE"
                     /locus_tag="ACHOQW_003404"
     CDS             7391..7684
                     /gene="ybfE"
                     /locus_tag="ACHOQW_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP9574429.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7824..8354
                     /gene="fldA"
                     /locus_tag="ACHOQW_003405"
     CDS             7824..8354
                     /gene="fldA"
                     /locus_tag="ACHOQW_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP9574430.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8564..8650
                     /gene="uof"
                     /locus_tag="ACHOQW_003406"
     CDS             8564..8650
                     /gene="uof"
                     /locus_tag="ACHOQW_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP9574431.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8643..9089
                     /gene="fur"
                     /locus_tag="ACHOQW_003407"
     CDS             8643..9089
                     /gene="fur"
                     /locus_tag="ACHOQW_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP9574432.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9173..9499)
                     /gene="chiQ"
                     /locus_tag="ACHOQW_003408"
     CDS             complement(9173..9499)
                     /gene="chiQ"
                     /locus_tag="ACHOQW_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP9574433.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9549..10954)
                     /gene="chiP"
                     /locus_tag="ACHOQW_003409"
                     /pseudo
     CDS             complement(9549..10954)
                     /gene="chiP"
                     /locus_tag="ACHOQW_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2756 a         2779 c         2812 g         2955 t
ORIGIN      
        1 tatctggggt atgatcgttt tttgttgatc ttcttcacag attatagcca tttcatggat
       61 agaataactc taccttcaac tgacacagca agaggtaaag gtaaatggaa aataataacc
      121 gcttaatgcc tcatataagg cggacaaccc atatcatgaa gtttgcccat cgtaatagtt
      181 ttgactttca tttctttaat gcccgctagt cttctgacta aagggcaccc cagcgtacag
      241 gtctccctga ctttaagcat tacaggttaa tacctgtatc cctcggtgct catatactgc
      301 taaccctttt aaactctaca taattcgagt cgcagcatat gcaacttgaa gtatgacgag
      361 tatagccagt taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt
      421 atttatcagc ggtggagctt tgcttttttt ccggcgtgat cgatttctcc tttgagaaat
      481 tgaggacctg ctattacctg aaataaagag atgaacaaaa tgtcagaatt aaaaattgcc
      541 gttagtcgtt cttgcccgga ttgtttttcc actcatcgtg catgcgtgaa tatagacgaa
      601 agtaattata ttgacgttgc cgccattatt ttatcagtca gtgatgttga acgtggaaaa
      661 ctcgatgaaa tagacgctac tggctatgac attcctgttt ttattgcaac ggaaaatgaa
      721 gaacgtatac cagcagaata tctttcacgt atttctggtg tcttcgaaca tggagaagca
      781 cgcaaagaat tttatggtcg tcagttagaa accgctgcca gccattatga aactcaactg
      841 cgcccacctt tctttcgcgc actggtcgat tatgtcaatc agggcaacag cgcatttgac
      901 tgccccggtc atcagggcgg cgaatttttc cgtcgccatc cggcggggaa tcagtttgtg
      961 gaatactttg gtgaggcgct gttccgtgcc gacttgtgca acgccgacgt agcgatgggc
     1021 gatctgctga ttcacgaagg cgcgccatgc attgcacagc aacatgcagc aaaagtgttt
     1081 aatgccgata aaacctactt cgttttaaat ggcacttcat cttctaacaa agtggtttta
     1141 aacgccctgc tgacaccggg tgatctggtg ctatttgacc gcaataacca taagtctaac
     1201 caccacgggg ccttgctaca ggctggtgca acaccggttt atctggaaac agcacgtaac
     1261 ccgtatggtt ttatcggtgg tattgatgca cactgcttcg aagaaagtta tctgcgtgaa
     1321 ctgattacag aagttgcacc gcaacgggca aaagaggcgc gtccgttccg cctcgctgtg
     1381 attcagctag gcacctacga cggtacgatt tataacgccc gccaggtggt ggataaaatt
     1441 ggtcatctgt gtgactacat cctgtttgat tccgcatggg tcggctatga acagtttatt
     1501 ccgatgatgg ctgactgttc gccgctgttg ctggatctta atgagaacga tccgggtatt
     1561 ctggttacgc aatccgtaca taaacagcag gctggttttt ctcaaacttc acaaatccat
     1621 aaaaaagaca gccacatcaa agggcaacag cgttatgtac cgcacaaacg catgaacaac
     1681 gcctttatga tgcatgcctc caccagcccg ttctatccgc tgtttgccgc actggatatc
     1741 aacgccaaaa tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat
     1801 ggcattaatg cccgcaaact gatcctcgat aactgtcagc atattcgtcc gttcatacct
     1861 gaactggtgg atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg
     1921 cgcttcttcc agtttgtacc aggtgagcac tggcattctt ttgaaggcta tgcagagaat
     1981 caatactttg ttgatccgtg caaactgttg ctgacaaccc caggtattga tgcacgtaac
     2041 ggcgaatatg aagcgttcgg tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat
     2101 ggcgtagtgc cggaaaaatg cgatcttaac tccatcctct tcctgctgac tccggcagaa
     2161 gatatggcca aacttcagca acttgttgcc ctgctggtac gcttcgaaaa actgctggaa
     2221 tctgatgcgc cgctggcgga agtgctacct tccatctaca aacagcatga agagcgctac
     2281 gccggttata cccttcgtca gttgtgccag gaaatgcatg atttgtatgc ccgccacaac
     2341 gtgaaacaac tgcaaaaaga gatgttccgt aaggaacact tcccacgcgt cagcatgaat
     2401 ccgcaagaag ctaactacgc ctatttacgc ggtgaagtgg agctggttcg tctgccggat
     2461 gcagaaggcc gtatcgctgc cgaaggtgcg cttccttatc ctccgggtgt gctgtgtgtt
     2521 gttccgggtg aaatctgggg tggtgctgtt ttgcgttact tcagcgctct ggaagaaggg
     2581 atcaacctgc tgccaggttt tgcaccggag ctgcagggtg tctatatcga agaacatgat
     2641 ggtcgtaagc aagtttggtg ctatgtcatc aagcctcgtg atgcgcaaag caccctgctg
     2701 aaaggggaaa aattatgagt caggctaaat cgaacaaaat gggcgtcgtt cagttaacca
     2761 tactgacgat ggtcaacatg atgggctccg gtatcatcat gctgccgaca aagcttgccg
     2821 aagttgggac aatctcaatt atctcctggc tggtgacagc cgtaggctca atggcactgg
     2881 catgggcatt cgcaaaatgc ggtatgttca gccgtaaatc aggcggtatg ggcggttatg
     2941 ccgagtatgc tttcggtaaa tccggtaact ttatggcgaa ctatacctac ggcgtctcac
     3001 tgctgattgc taacgtcgcg attgctattt cggcggttgg ttacggcacc gaactgctcg
     3061 gcgcgagttt gtcgccagtg cagattggtc ttgcgaccat cggggtgctg tggatttgta
     3121 ccgtggctaa ctttggtggt gcgcgcatta ccgggcaaat cagtagcatt actgtgtggg
     3181 gagtcattat tccggtcgtc ggtctgtgca ttattggctg gttctggttt agcccgacgc
     3241 tgtacgttga ttcctggaat ccgcatcatg caccgttctt cagtgcggta ggttcttcca
     3301 tcgctatgac gctgtgggct tttcttggtc tggagtctgc gtgtgcaaat actgatgtag
     3361 tggaaaaccc ggaacgtaat gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg
     3421 tgatttatat cgtctccacc aacgtgattg ccgggattgt gccaaatatg gagctggcaa
     3481 attcaacggc accatttggt ctggccttcg cgcagatgtt cacgccggaa gtgggtaaag
     3541 tcattatggc gctgatggtg atgtcctgct gcggttcgct gcttggctgg cagttcacca
     3601 ttgcccaggt gtttaaatct tcatctgatg aaggctactt ccctaaaatt ttctcccatg
     3661 taaccaaagt ggatgcaccg gtgcagggaa tgttgaccat tgtgattatc cagagcgggt
     3721 tggcgctgat gacgattagc ccgtcgctaa acagtcagtt caacgtgctg gttaacctgg
     3781 ccgtggtgac caatatcatt ccgtatattc tgtcaatggc ggcattagtc attattcaga
     3841 aggtcgctaa tgtgcccccc tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg
     3901 cgatgtacag cttttatgcg ctgtactcat ccggggaaga agccatgctg tacggttcca
     3961 tcgtgacctt ccttggttgg acactgtatg gtctggtatc accacgcttt gaactgaaaa
     4021 ataaacacgg ttaaaaaagc aaagtaaaaa agggcgatct tgcgaccgcc ctttttctta
     4081 tttaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct tctttctcaa
     4141 tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat gcgtcttccg
     4201 tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga ccgccaatag
     4261 aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacca gccagggtgc
     4321 tggcactcac catttccgga gacagcttag acagcgccgc tttttgtgcg gaagtcgcag
     4381 ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg ttgtagtgtt
     4441 cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac atgatgatgc
     4501 cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc cctgcgctct
     4561 cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat ttgaaaccta
     4621 ccgggacttc taccagttta cggcccaagt cgttgaccac acggtcgatc atcgcagatg
     4681 aaaccagcgt tttaccgacg gcaacagctt tgccccactg cggacgatgc tggaacaggt
     4741 agttgattgc caccgccagg tagtggttcg gattcatcaa acctgccgga gtgacgatac
     4801 cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca cgcagtgcca
     4861 gcaggcccgc catcgcacac tcggaggagc agtccatacg gatcgcgccg tctttatcaa
     4921 ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg ttgaggttgt
     4981 aatactcgcc aatacgcttc cagtattcga taccggaacc gcccagcgga tcaacgccaa
     5041 gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc ccttccacaa
     5101 acggctgcac cagatcctgc tctttcacat gaccggatgc catcgcttcg tcgagggaga
     5161 tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggctctgtct tccaccactt
     5221 tagtgacgtt ggtatcagcc gggccaccat ttggcggatt gtacttgata ccaccatctt
     5281 ccggcgggtt atgggacggt gtaataacga taccgtctgc cagcgggcca ccttttttat
     5341 tgtgaaccag gatggcattg gaaacggcag gcgtcggggt gaagccattg ttttcctgca
     5401 caataacatc tacgccgttc gctgccagca cttccagaac ggaaataaat gcaggttcgg
     5461 acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt ttcgcacgtt
     5521 cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg tggcgcgctg
     5581 cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc cctgcttctg
     5641 gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca ctctgttgtg
     5701 caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc aacgttttag
     5761 atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg catgatgtgt
     5821 tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata cggcgtgcct
     5881 ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc atctgccgca
     5941 aagtaaacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc ctgggcgtca
     6001 agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt ttgctctgca
     6061 tattcatccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat cgttttgatc
     6121 ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac cggagcagca
     6181 ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc gctctcgccg
     6241 atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc aatcgttttc
     6301 atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caggagtata tgaataaatc
     6361 ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac cttatttgtt
     6421 gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga aaaaaagaac
     6481 tttgccatga aattgaatat ccgcgcgcaa actgcacaaa accagcacaa taattctccc
     6541 atcgttcttg tccatggtct gtttggcagc ctcgacaacc ttggcgtact ggctcgcgat
     6601 ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct ttcaccgaga
     6661 gatccggtaa tgaattaccc ggcgatggcg caggatcttg ttgatactct ggatgcacag
     6721 cagatcgaca aagcaacatt tatcgctcac tccatgggcg gtaaagcggt aatggcactt
     6781 actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc accggtcgac
     6841 tatcacgtac gccgtcatga tgagattttc tcggctatca acgcggtcag tgaatcggac
     6901 gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga agaaggggtg
     6961 attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt gccggtattg
     7021 tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga tcaccctgcc
     7081 ctgtttatcc ctggtggtaa ttctccgtat gttagcgagc agtaccgtga tgatttactg
     7141 gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg ggtccatgct
     7201 gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta acgcaactga
     7261 ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac tcatcgcgct
     7321 gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt cccccgaagt
     7381 caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt tcgcgcacga
     7441 gcgtcgaccg ggacgaccga aaacaaatcc gctttcgcgc gatgaacagc tgcgtattaa
     7501 taaacgcaac caactaaaac gcgacaaagt acgtggccta aagcgtgtcg aactgaagct
     7561 gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgcaata tgagccgtag
     7621 cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc agggcatcgt
     7681 ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg attccgcaag
     7741 actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc catttcaata
     7801 agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc agcgacaccg
     7861 gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac gttgccgatg
     7921 tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttatgacatt ctgctgctgg
     7981 gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc ttcccgactc
     8041 tcgaagagat tgatttcaat ggcaaactgg ttgcgctgtt tggttgtggt gaccaggaag
     8101 attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt gaaccgcgcg
     8161 gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca tcaaaaggtc
     8221 tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag ccggaactga
     8281 ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat ctcgacgaaa
     8341 ttctcaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta atagataatg
     8401 ccaatcaaaa taattgctac aaatttgtaa cttttgccgt tgtacctgta caatgtcccg
     8461 gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat taacaatatt
     8521 tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac gcattatctc
     8581 aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag gacagattcc
     8641 gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg cttcctcgtt
     8701 taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg gaagatttat
     8761 acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat cgcgtactga
     8821 accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt aaatccgtat
     8881 ttgaactgac acagcaacat caccacgatc acctgatctg cctcgactgc ggcaaggtta
     8941 tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca aaacatggca
     9001 ttcgcctgac taaccacagt ctctatcttt acggtcactg cgccgaaggc gattgccgcg
     9061 aagatgagca cgcgcacgaa ggcaaataag ccagtctgaa cgagaaaagc caacctgcgg
     9121 gttggctttt ttatgcaagg gaaataagaa aagcgagagt tacagctctc acttatttgt
     9181 tattactgcg aatttcctgc cagaccttat cacaatcggc tttcaccgcc tgatcatttc
     9241 cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcggacactt tcctggtcgg
     9301 caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg caggcttcaa
     9361 ttttttccgg cgaaccttgt gcggtgttga tacaggcgct gtatgcctct ttcagacggc
     9421 tgtcttcctg tggcgcggtt gattgcgcac aagctaccag ccccgatgcc atcatggcga
     9481 tgagaatcag ttttttcata gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc
     9541 gtcgggcatc agaagatggt gaatggtgcg attaccataa attttacgtc acgctcatcc
     9601 tggaagatgt tgccgtaacc gccgccccag cttgggatgt cggagtggtt gtcgtattcg
     9661 gtgaagtgca gtttgaacat cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca
     9721 tccaggctgt atgcagactc ttcaatagtc cggtttttgt cgtagtacgc atccggattg
     9781 ctctgccagg tcgcaggttt agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag
     9841 cctggaagat tccagttttt caggtcatac atcgcaccga agaagaccgc tttttcgccg
     9901 ttggcgttga agtcagaacg gttatcccac cagatatcca ggcgaccgtt cgaggaagcg
     9961 taggttggag tcatacgttg caggaagtat ccctgctgac cgtcagcttt aacccaggtg
    10021 ccttcgaggc gcaaatccac tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc
    10081 caggcggtgc cgtcataaag atcgttgacg ctgcgatcgt caactttatc gcgcgtaccg
    10141 tagaactggt agctggtggt taacgggcta ccggcgatat caaatttgta gctggctttg
    10201 gcaaagtatt gatcgatata cccttccgcc tgaccaaatg ccgcttccag tacgaagtta
    10261 tttttgaagt cgtatttcgc cccaagggag tgcagataat caactttggt ggttttatcg
    10321 ttctgataaa actcatccat ttccagatgc cacggtgctt tgtattcgtt ggtccacatg
    10381 taggagaaac tcaacgcacc agcatcgccg taatcaaaat tcgccccggc ctccgcaccc
    10441 tgataagtac ccggcataaa gctccagtgc ggcgctaaca gcgtttgacc agttggctga
    10501 atataacctg ccctcgccac accggaccgt atttaaattt ggccgctgct ttatacaggc
    10561 ttataccgct tttatcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg
    10621 cgatttcgtt cgggtgagag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcgg
    10681 caatatcaag gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcgttcc
    10741 aggtggagtg agaaaggttg gttttgtatt tatcgccgtc ggtaacatct ttacggtcgc
    10801 gttcacgctg ccagtaatag ataccgccgg ttaaggttga atcgtcaatg aatccttcgg
    10861 cgcgagcctc cggcattgcc ataaagcccg acattgctgt aacaccggcg atagccagcg
    10921 ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat
    10981 tgctgcacca caggtgaaca gcgaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag
    11041 tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta
    11101 accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta
    11161 agtttgcggt tgaaaggctt gcagaagaat aaaagcgtga aattccagca agatgaaagc
    11221 ggttacattt tatctaatta tgttttgtgt ttttaaattt attttatgga taaattgcga
    11281 agcggaatta attcgcgaag aa
//
LOCUS       DAZKFZ010000147        11205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000147 DAZKFZ010000000
VERSION     DAZKFZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(252..2081)
                     /gene="glmS"
                     /locus_tag="ACHOQW_003410"
     CDS             complement(252..2081)
                     /gene="glmS"
                     /locus_tag="ACHOQW_003410"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9574434.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2243..3613)
                     /gene="glmU"
                     /locus_tag="ACHOQW_003411"
     CDS             complement(2243..3613)
                     /gene="glmU"
                     /locus_tag="ACHOQW_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9574435.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3965..4384)
                     /gene="atpC"
                     /locus_tag="ACHOQW_003412"
     CDS             complement(3965..4384)
                     /gene="atpC"
                     /locus_tag="ACHOQW_003412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9574436.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4405..5787)
                     /gene="atpD"
                     /locus_tag="ACHOQW_003413"
     CDS             complement(4405..5787)
                     /gene="atpD"
                     /locus_tag="ACHOQW_003413"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9574437.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5814..6677)
                     /gene="atpG"
                     /locus_tag="ACHOQW_003414"
     CDS             complement(5814..6677)
                     /gene="atpG"
                     /locus_tag="ACHOQW_003414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9574438.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6728..8269)
                     /gene="atpA"
                     /locus_tag="ACHOQW_003415"
     CDS             complement(6728..8269)
                     /gene="atpA"
                     /locus_tag="ACHOQW_003415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9574439.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8282..8815)
                     /gene="atpH"
                     /locus_tag="ACHOQW_003416"
     CDS             complement(8282..8815)
                     /gene="atpH"
                     /locus_tag="ACHOQW_003416"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9574440.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8830..9300)
                     /gene="atpF"
                     /locus_tag="ACHOQW_003417"
     CDS             complement(8830..9300)
                     /gene="atpF"
                     /locus_tag="ACHOQW_003417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9574441.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9362..9601)
                     /gene="atpE"
                     /locus_tag="ACHOQW_003418"
     CDS             complement(9362..9601)
                     /gene="atpE"
                     /locus_tag="ACHOQW_003418"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP9574442.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9648..10463)
                     /gene="atpB"
                     /locus_tag="ACHOQW_003419"
     CDS             complement(9648..10463)
                     /gene="atpB"
                     /locus_tag="ACHOQW_003419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9574443.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10472..10852)
                     /gene="atpI"
                     /locus_tag="ACHOQW_003420"
     CDS             complement(10472..10852)
                     /gene="atpI"
                     /locus_tag="ACHOQW_003420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP9574444.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2692 a         3036 c         2770 g         2707 t
ORIGIN      
        1 ttacatattg atgattgttt tacaagcgat tcttgaatga acaaaacgtg ctattcatta
       61 caaagtacat caattatggc aattgtttgt caatgattcg aggtatttaa ttactactac
      121 ggttaaaaca agtgttcaac ggtgccccaa ataatagctg aagttaatta tatggaatga
      181 ggttagatta ttaatgctgg taagtaaaag ggccgccaaa aggcagccct tcgttcagca
      241 aatgaataat attactcaac cgttaccgat tttgccaggt tacgcggctg gtcaacgtcg
      301 gtgcctttga tcagcgcgac gtgataagcc agtaactgca gcggaacggt gtagaagatc
      361 ggtgcaatca cctcttccac atgcggcatc tcgatgatgt gcatgttatc gctacttaca
      421 aaacccgcat cctgctcggc gaagacatac aattggccgc cacgcgcgcg aacttcttca
      481 atgttggatt tcagtttttc cagcaattcg ttgttcggcg caacgacgat aaccggcata
      541 tcggcgtcaa tcagcgccag cggaccgtgt ttcagttcgc ctgcagcgta ggcttcagcg
      601 tgaatataag agatctcttt cagcttcaat gcgccttcca gcgcgattgg gtactgatcg
      661 ccacggccca ggaacagcgc gtgatgtttg tcagagaaat cttctgccag agcttcaatg
      721 cgtttgtcct gagacagcat ctgctcaata cggctcggca gcgcctgcag accatgcaca
      781 atgtcatgtt caatggaggc atccagacct ttcaggcgag acagcttcgc caccagcatc
      841 aacagcacag ttaactgagt ggtgaatgct ttagtggatg ccacgccgat ttccgtaccc
      901 gcgttggtca tcagcgccag atcggattcg cgcaccagag aagaacccgg aacgttacag
      961 attgccagcg aaccaaggta acccagctct ttcgacagac gcaggcccgc cagggtatcc
     1021 gcggtttcgc cagactgtga caaggtgatc atcaggctgt tacgacgcac ggcagattta
     1081 cgatagcgga attcagaggc gatttcgacg tcgcacggaa tacctgccag cgattcaaac
     1141 cagtaacggg aaaccatacc ggagttataa gaagtaccac aggcgaggat ctgaatatgc
     1201 tcaaccttcg acagcagttc gtcggcgttc ggtcccagct cgcttaaatc aacctgaccg
     1261 tggctgatgc gcccggtaag ggtgtttttg atcgcgttcg gctgttcgta gatctctttc
     1321 tgcatgtagt gacggtaaat acctttatcg cccgcgtcat attgcagatt ggattcgata
     1381 tcctgacgtt ttacttccgc gccagtttta tcgaagatgt ttaccgaacg gcgagtgatt
     1441 tccgcaatat cgccctcttc aaggaagata aagcgacggg tcaccggcag cagcgccagc
     1501 tggtcagaag cgataaagtt ttcacccata cccagaccaa tcaccagcgg actaccagaa
     1561 cgtgctgcca gcagggtatc cgggtgacgg gagtccatga tcactgtacc gtacgcacca
     1621 cgcagttgcg ggatagcacg cagaacagcc tcacgcagag taccgccctg tttcagctcc
     1681 cagttcacca gatgggcaat cacttcggtg tcggtttcag aaacgaaggt atagccacgc
     1741 gcttttaact cttcgcgcag cggttcatgg ttttcgatga tgccgttatg caccaccaca
     1801 atgtgttcag aaacatgcgg atgcgcattc acttctgaag gttcaccgtg ggtcgcccag
     1861 cgagtgtgag caataccggt gccgccatgc agaggatgtt cttccgctgc ctgagccagc
     1921 atctggactt taccgaggcg acgcaggcgg gtcatatgac cttccgcatc aacaacggcc
     1981 agaccggcag agtcatatcc gcggtattcc agacgacgta aaccttcaag aaggatttct
     2041 gctacatcac gttgcgcgat cgcgccaaca attccacaca tagtttttga ttccgattta
     2101 tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg ccccgagcct tgtagagagt
     2161 ggggttattt ttatagttac tgcttgtggg agggagatta tgttatctcc tcatcccatg
     2221 tgaccggatt acccggccag aatcactttt tctttaccgg acgacgccag ccttctttct
     2281 gagtctgcgg cacacggctg atagctaatg cattttcgcc gacattacgc gtcacagttg
     2341 tacccgcagc aatggtcgcg cctttgccta ctgttaccgg ggccaccagc tgagtgtcgg
     2401 aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt attcgcacca tcgtagttgc
     2461 aggtaatggt tcccgcgccg atgttaacgt tatcgccaat ttccgcatcg ccaaggtaag
     2521 tcagatgacc agctttcgag cctttaccca gacgtgcttt tttcatctca acaaagttac
     2581 cgacgtgtgc accttccaac aactcagcac caggacgcaa acgggcaaac gggccaatgg
     2641 tacaggccgc cgccagattc gcgtcttcca cgacggtata tgggctaatt tcgcaatcat
     2701 cgccaatcac gctgttttta atcacgcaac cggtgccgat tttcacgcga tggccgagag
     2761 tcacgttgcc ctcgatgata acgttagtat caatttcaac atcgcgcccg tgagtaagcg
     2821 taccgcgcag atcaaaacgc gccggatcgc gcagcataac gcctgctaac agcagttttt
     2881 cagcctgttc ggactggtaa acgcgctcca gacgggagag ttgcaggcgg ttattcacgc
     2941 cttctacttc gcttaaacgt tgcggatgaa cggcgacgat ttcacgccct tcctgatacg
     3001 ccagcgcaat aatgtcggtg atgtagtatt cgccctgcgc attattgttg gtcagcttcg
     3061 ccagccagcg tttcatatct gcgccgttag cgatcagaat gccggtgttg atctcctgaa
     3121 tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat gccggtaact ttgccgtttt
     3181 cacgggtgat acgtccataa ccggtcggat catccagttt caccgtcagc agaccaatgc
     3241 caccctgcgg tttagcatca cgcagacgct ggagtgtttc gacagagatc agcggcacgt
     3301 cgccgtagag cattaaaatg tcttcatcat cggcaaagaa aggtgcggcc tgctgcatgg
     3361 catgacccgt acccagctgc tctgcctgaa gcacccagtt caggttgtcg tctttcagcg
     3421 cctgttttag cagatcgcca ccgtgaccgt acaccaggtg aacgtgcgct gcgcctaatt
     3481 cattcgcagc atcaatgaca tgctgaacca tcgctttccc ggcaagggta tgcagcactt
     3541 tcggaagatc ggaatacatg cgcgtgcctt tacctgcggc aaggatcact acgctcatag
     3601 cattattcaa catacgcgtc ctgactgtaa tttgagaacg aatttaaacc gcttcacctt
     3661 gaaaaaacta catttttttc atcgtgaaat ggacagagga taaattgttc aataacggat
     3721 tatccccgct gacgaaagcg ccattttcga ccatcgtcac gccttttgtc tgcagaaaat
     3781 taaggcagta ccttcattta agcgtcaggt ggatgttttt gctcttattt cgatcaatga
     3841 ataaacggga aaacaggggg ttttatatca gcaggatcta tgtgaacgct attcaggacg
     3901 ggtcacacgc gcaaaaaaaa gccagcctgt ttccagactg gcttttgtgc ttttcaagcc
     3961 ggtgttacat cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc gctttggcca
     4021 gttccgcaga cgcctgagcg taatctacgt cgccgtgaga gctgctaatg tgctcttcag
     4081 ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt
     4141 cggccagaac ggtcacgttg ccaggctgca cttcaagaat gccgccagac agatagataa
     4201 actcttcgtg accgtgctgt ttcacgatgc gaatcatacc aggcttaatg gcggtgagca
     4261 gcggtgcgtg gccagggtag atccccagtt caccttcgct acccgttacc tggattttct
     4321 cgaccagacc agagaacatt tgttgctctg cgctgacgac gtccaggtgg taagtcattg
     4381 ccatatcacc ctccgattaa ggcgttaaag ttttttggct ttttccacag cttcttcgat
     4441 ggaaccgacc atgtagaacg cctgctccgg caggtgatcg tattcgcctt ccatgatgcc
     4501 tttaaagcca cggatggtgt ctttcaggga gacgtattta cccggagaac cggtgaatac
     4561 ttctgccacg aagaacggct gggacaggaa gcgctggatc ttacgagcac gcgctaccac
     4621 cagtttgtct tcttcagaca gttcatccat acccaggatg gcgatgatgt ctttcagttc
     4681 ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac
     4741 caccagcggg tccagctgac ggctggtgga gtccagcggg tcaacggccg ggtagatacc
     4801 cagagacgcg atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc
     4861 cggagacggg tcagtcaagt catccgcagg tacgtatact gcctgtacgg aggtgataga
     4921 accggttttg gtggaggtga tacgttcctg cagaacgccc atctcttccg ccagggtcgg
     4981 ctgataacct accgctgaag gcatacggcc cagcagtgcg gatacttccg taccggccag
     5041 ggtgtaacga tagatgttgt caacgaacag cagaacgtca cgaccttcgt cacggaattt
     5101 ctcagccatg gtcagaccgg tcagagctac gcgcagacgg tttcccggcg gctcgttcat
     5161 ctggccatac accagggata ctttatcgat aacgttggag tcggtcattt cgtggtagaa
     5221 gtcgttcccc tcacgagtac gttcacctac gcccgcaaac acagagtaac cggagtgctc
     5281 gatcgcgatg ttacgaatga gctccatcat gtttacggtt ttacctacac ccgcaccacc
     5341 gaacagacca actttaccgc ccttagcgaa cggacacatc aggtcgataa ctttgatacc
     5401 ggtttccagc agttcctgag agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat
     5461 cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg accggttcac ccagtacgtt
     5521 catgatacgg cccagagtcg ctttacctac cgggacttcg atcgggtgtt cgaggtcttt
     5581 tacatccaga ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac gcacgatacc
     5641 gccgccgagc tgctgctgaa cttccagcac cagacgctca ttaccatttt gcacctcaag
     5701 agcatcgtac acgcgcggta cggcatcctg agggaattcg acgtcaacta cggcgccgat
     5761 tacctggaca atctttccag tagccatctt aaatcctcta cgaaataacc tgtttaaacc
     5821 gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg acgagctttg
     5881 ttgtatacca actgcagctc tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc
     5941 gccaccatac gggcggcctg ctcgctggcc aggttttcaa ccacgccctg ataaacctga
     6001 gattcgacat aacgacgcag cagggtatcc agcaacgcct tcggatcggg ttcgtacagg
     6061 taatcccagg atttatgttt cagatcatca tcatctgatg ccggtaacgg cagcagctgg
     6121 ctgatggtcg gaacctgaga catggtgtta ataaatttgt tgctgacaat gtaaagcttg
     6181 tccagacggc cttcgtcgta ggcctgcaac atcactttta ccggaccgat cagttcggac
     6241 agggaagggt tatcccccat gccggtgacc tgggcaacaa cattgccgcc cacggagttg
     6301 aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc attgaacgcc tttgtcggtc
     6361 caggtcttca tttccgccag cagttttttg aacaggttaa tgttcaaacc accgcacaaa
     6421 ccacggtcgg tcgacaccac caggtagccc acgcgtttaa cgtcgcggtc ttccaggtaa
     6481 gggtgcttat attccagatt accgtgtgca aggtgaccaa tcactttgcg catggtttct
     6541 gcataaggac ggctggccgc catgcgatcc tgcgatttac gcattttgga agcggcgacc
     6601 atctccatcg ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt actacgtatc
     6661 tcttttgcgc cggccatgag cttctcctca atgccttgcg gcctgcccta aggcaagccg
     6721 ccagacgtta ccaggattgg gttgctttga aggaatcgag gatgcctttc agcttgcctt
     6781 cgatttcgtc gttgtagcca ccggtctggt tgatctcttg catcaacgga gcgtgatcac
     6841 ggtcgacgta agccagcaga gcggcttcga agctgccgat tttcgacagt tcaacatccg
     6901 ccaggtaacc acgttctgct gcgaacagaa ccagagactg ctgcgcaacg gacatcggcg
     6961 catactgttt ctgtttcagc agttcggtca ctttttgacc gtggtcaagc tgcttacgtg
     7021 ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc cagttcacga tactgtgcca
     7081 gagcggtacg gataccaccg gacagttttt tcatgatctt ggtctgtgct gcaccaccaa
     7141 cacgggatac ggaaataccc gggttaaccg caggacgaat accggcgttg aacaggttgg
     7201 tttccaggaa gatctgacca tcggtaatgg agattacgtt ggtcggaacg aacgcagaaa
     7261 cgtcacccgc ttgagtttcg ataatcggca gcgcggtcag tgaaccggtt ttccctttca
     7321 cttcaccttt ggtgaaagct tcaacgtatt cggcgttaac acgcgcagca cgctccagca
     7381 gacgagagtg gaggtagaat acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga
     7441 gcagcagcga gatctgacgg taagcaacag cctgtttaga cagatcatcg taaatgatca
     7501 gcgcatcttc accgcggtca cggaagtatt cgcccattgc gcaaccggca tacggtgcca
     7561 ggtattgcag tgcagcggat tcagacgcgg ttgctaccac aacgatggtg ttagccagtg
     7621 cgccgtgctc ttccagttta cgtaccacgt tagaaatggt ggacgctttc tggccgatag
     7681 cgacatagat acatttgata ccggaatcgc gctggttgat gatggcatcg atagccagtg
     7741 cggttttacc ggtctgacgg tcaccgatga tcaattcacg ctgaccacga ccgattggga
     7801 tcatggagtc aacggcttta taaccggtct gtaccggctg atctacggac tgacgttcga
     7861 taacgcccgg agcgattgct tctacagcag agaagccgtc gtgatccagc ggacctttac
     7921 cgtcgattgg tgcacccaga gtgttaacca cacggcccag caggccacgg ccaaccggaa
     7981 cttccaggat acggccagta cacttaactt tcatgccttc ggcaaggtca gcatacggac
     8041 ccataacaac cgcaccaaca gagtcgcgct cgaggttcag tgcgatagcg taacggttac
     8101 ccggcaggga gatcatttca ccctgcatac aatcggccag gccgtgaatg cggataacac
     8161 cgtcacttac agaaacaata gtaccttcgt tgtgagcttc actcacaaca ttgaactgag
     8221 caatgcgctg cttgatcagt tcgctgattt cggtggaatt cagttgcatg ctccagtccc
     8281 cttaagactg caagacgtct gcaaggcgct caagacgacc gcgtacgctg ccatcaatga
     8341 ccatatcacc cgctcggatg ataacgcctg ccattacaga cttatcgatt ttgcaattca
     8401 gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg agctgttgtt
     8461 cactcagtgc ggcagcggaa atgacgtcta cctcagcggt agcctcactc acggcacgaa
     8521 ggtgaataaa ctgctccaaa acatccggga gcgcgttaag acgaccattt tcagccatta
     8581 cccgaataag gttctgaccg ttttcgtcca gttgctcacc acaaactgcg ataaacgact
     8641 cggcaagcgt ttctggcgca agcgcgccag agagaagctc tgccatttgt tcgtttttgg
     8701 ttacctcggc ggcaaacgcc agcatgtcct gccagcgttc tacactttgg tgttcgacgg
     8761 caaagtcaaa agctgctttg gcgtaggggc gagctaccgt aataaattca gacatcagcc
     8821 cctccctcct tacagttcag cgacaagttt atccacgatg tcgctgttag cagcttcatc
     8881 cacggaacgt tcgatgatct tctcggcgcc agcaacagcc aggatagcaa cttgcttacg
     8941 cagctcttca cgggcacgtt tacgctcggc ttcaatttcc gcctgcgcct gggccacgat
     9001 tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt
     9061 gttcgcctgc tcgatgatta cctgggcttc cgctttcgct tttttcagct ggtcggtcgc
     9121 gctggccttt gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc
     9181 aatttctttt tgacgttttt cgatggctgc cattaatggc ggccatacgt acttcatgca
     9241 gaacagaacg aacaggacaa acgcgatggc ctggccgagg attgttgcgt taagattcac
     9301 agcacaatgc ctctatttag ttaacgttct gatattgctc tttaaataaa agcaacgctt
     9361 actacgcgac agcgaacatc acgtacagac ccagacctac agcgatcatc gggatagcat
     9421 ccaccagacc cataacgata aagaactgag tacgcagcag aggaatcaga tcaggttgac
     9481 gcgctgcgcc ttccaggaat ttacccccga ggatgccgat accgatcgca gcaccgattg
     9541 ccgccagacc catcatcaca gcggcagcca tgtacagcag atccatattc aggttttcca
     9601 tgacagtctc cagtttgttt cagttaaaac gtagtagtgt tggtaaatta atgttcttca
     9661 gacgccatcg acagatagac gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg
     9721 atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc actgtgacca ccacggcaac
     9781 agaccagcaa tcagaatgaa aatcagctca ccggcataca tgttaccgaa cagtcgcaaa
     9841 ccgagtgaaa ctggtttgga cagcaggctt accccttcaa ggattaagtt gacaggaatg
     9901 aacgcccagt gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc
     9961 attttgatgc tgtagaacag aatcaggata aatacgccca gtgccataga cagcgttacg
    10021 ttcacgtccg cagacggaac cacacgcagt gcaggcagac ccagtacatg ttcagcaatg
    10081 tacggcagca ggtcgatagg cagtaaatcc atcaggttca tcaggaatac ccagacgaag
    10141 atcgtcaggg ccagcggagc aatcagcttg cttttgccat ggtacatgtc tttcacgcta
    10201 ccattaacaa agccgatcac cagctcaatc gcggtctgaa acttacctgg cacaccgctg
    10261 gtcgcctttt tggctacgct acggaataaa accaggaaca acagacccag caccaccgag
    10321 aagaacatgg agtcaatatt gattgtccag aaggtggctg gggggttttg tggatccacc
    10381 agcgagaatg tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc
    10441 ggcgtcatat tttctgaagc catgatgcct tttacccttt gttgttaatt acagccggtg
    10501 ccagtatctg aaccaccagc accaaaaccc acgtaacgat cagcggcaag aataccgcct
    10561 ttaaaaccgc caacgccacc accagtaaca ccaacatcgc cagaactttg aaagcttcac
    10621 caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt atgcgcctgg tgacgccagg
    10681 caaatatcat aaacaaaacg ttaggcagaa agactgccag gcccccgctt attgcagaga
    10741 cgccccagaa ggggtctttg aggctgaaca gcaatccact tgctatcacc accagtaact
    10801 gaacgagcag aagcttccga gcaacgtttc gactcacgag cgacacagac atcacgtttt
    10861 tcactcctgc tcccttcgag gtatgccgcg tgtcgtataa aactttcttt aaggcttaga
    10921 gtcaagcatc aaaaagcggt caaattatac ggtgcgcccc cgtgatttca aacaataagt
    10981 agccaaaagg tgaataaatg tttaaatatt tttccagtgc atacaattgc gacttttcta
    11041 ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta aacactggtg ataaagcgtg
    11101 cttcagatca catattgcgc atgttcgcgc acagcatatt tatttacttg gcaaatgatg
    11161 cctttgcaag tttatgatat ttcagtctaa aaacagatac tgttt
//
LOCUS       DAZKFZ010000148        11153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000148 DAZKFZ010000000
VERSION     DAZKFZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            9..914
                     /locus_tag="ACHOQW_003421"
     CDS             9..914
                     /locus_tag="ACHOQW_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9574445.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(948..1550)
                     /gene="cobO"
                     /locus_tag="ACHOQW_003422"
     CDS             complement(948..1550)
                     /gene="cobO"
                     /locus_tag="ACHOQW_003422"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9574446.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1560..3212)
                     /locus_tag="ACHOQW_003423"
     CDS             complement(1560..3212)
                     /locus_tag="ACHOQW_003423"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9574447.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3320..4600)
                     /locus_tag="ACHOQW_003424"
     CDS             complement(3320..4600)
                     /locus_tag="ACHOQW_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFP9574448.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4761..5078)
                     /locus_tag="ACHOQW_003425"
     CDS             complement(4761..5078)
                     /locus_tag="ACHOQW_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFP9574449.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5075..6445)
                     /locus_tag="ACHOQW_003426"
     CDS             complement(5075..6445)
                     /locus_tag="ACHOQW_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFP9574450.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6449..7690)
                     /locus_tag="ACHOQW_003427"
     CDS             complement(6449..7690)
                     /locus_tag="ACHOQW_003427"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFP9574451.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7690..9135)
                     /locus_tag="ACHOQW_003428"
     CDS             complement(7690..9135)
                     /locus_tag="ACHOQW_003428"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFP9574452.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9154..10542)
                     /gene="glmL"
                     /locus_tag="ACHOQW_003429"
     CDS             complement(9154..10542)
                     /gene="glmL"
                     /locus_tag="ACHOQW_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFP9574453.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLSSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10542..10991)
                     /gene="glmS"
                     /locus_tag="ACHOQW_003430"
     CDS             complement(10542..10991)
                     /gene="glmS"
                     /locus_tag="ACHOQW_003430"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFP9574454.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2643 a         2814 c         2812 g         2884 t
ORIGIN      
        1 acggcattat gcgagggaaa atacctaaaa cggaactgct ggtgactttt gaggtcgtcg
       61 cacgtcatga aagttatacc agagcggcag aggagttagc gttaacccaa agtgcagtat
      121 tcagacaagt aagtgcgctg gaggaatttt tacgtactcc gttattttca cactctaaaa
      181 aacgcatttt tcttaatgat acagggaagt attacctggg gatagtaaaa gaaacattaa
      241 ataagctgga acgcgatacc aataccatta tgacctggca gcctacggtt caggttattg
      301 aacttgccgt taatcctact tttagtaccc actggttaat cccaaattta catgaattca
      361 caaagttaca tcccgatatt attgttaata ttcattcttt agctaataac ggcgattttc
      421 ttaatcggga gtacgatgct gtcattatgc gcgaaaattt ttgcgcgccc tgggcggaag
      481 tagaatatct ctttgaagag gagattttgc cggtatgcag cggcagtttg cttgcaatgt
      541 ccgatcaaaa actgtcagtg gcagaactgc ttactgagtt accacttctg catcaaagca
      601 cccgtattac tggctgggaa gagtggtttg ctttatctgg cgttagcagt ccgctggtca
      661 acaacggacc acgtttcgat ttgctttcaa tgctgattgc cgccgtgcgc tctaatctcg
      721 gcgtggcgct gttaccacgt ttcgctattc aacatgattt ggatagtggc gatatggtga
      781 ttccctgtga tgtgcctatt cgtaccggta atcgttttat tatgacgtgg caggaagaga
      841 agtctgactc accgcacttg cagcaatttc gtgaatggtt gctggcgaaa tcggtcgtac
      901 cgcaggagat gtaaaacctg cgcctggcgg gatgccaggc gcagttatta ccagtcgata
      961 cccggctggg cctgaatgcc gttatcaaaa gcatgtttga cggggcggat ttcgctgacc
     1021 gtatctgcca tttttaatat ctgactgtgg caaccgcgtc cggtgacaat gacactttgt
     1081 tgtgccgggc ggttttgcag agaagcgatc acttcttcgg tatccagata atggtaagcc
     1141 agcatatagg tcagctcatc aagcaccact aagtcgtaac gcttgtcagc cagcatccgt
     1201 ttgctttccg accagacctc tttcgccgcg tcgatatccg cttgccggtt ctgtgtctcc
     1261 caggtaaaac cggttcccat aatatgaaac tcaaccccca gcggctggag aaggttgtat
     1321 tcgccgttgt cccattgccc cttgatgtac tgcgcaaccc ccaccgtttt gccatgaccc
     1381 accgcgcgcg tcacagtacc aaacgcggca gtggatttcc ctttgccgtt gccggtgaaa
     1441 acaatcaaga tccctttttt ctctatcgcc gccgcgacgc gcgtatcgac ctgttccttc
     1501 aatttctgtt ggcgctgcac atgccgttca gttgaaattc gcgcttccat tcattttcct
     1561 taatgtcccg cagggcaact ggcgcagcgg tgttgctgct caaactgact aaagaaatta
     1621 ttgcctttat catctaccag gatgaatgcg ggcagatttt ccacttccat catccacacc
     1681 gcttccatac ccagctccgg gtattccagg caacgcagac ttttgacata ttcttgcgcc
     1741 agtaacgcag ctgcaccgcc aatacttccc agattgaagc cgccatgttt gtgacaggca
     1801 tcggtgacct gctggctgcg gttacctttc gataacatca ctagactgcc gccagccgcc
     1861 tggaaggtat cgatataacc gtccattcga ccgcccgtgg ttggacccag tgaaccgcag
     1921 gccatgttct ccggtgtttt cgccgggcca gcgtaataga caatatgatg cttcaggtac
     1981 tccggcatcg gttcgccgct atccagccgc gcttttattt tggcgtgggc gatatcgcgc
     2041 gccactacga tgggaccact taacgacacg cgggtgccta ctggtaatct ggcgagatcc
     2101 tgcatcacgt cacgcaacgg acggtttaga tccagttgta catgttgcgc gtgattttct
     2161 tcccgcagtg aggctggaat atattgcccc ggattgtgtt cgagtttttc cagccagata
     2221 ccgtgtttgt taatttttgc tttgatattg cgatcggcag aacaggagag cgccatagct
     2281 atcgggcaag agccaccgtg acgcggcaga cgaatgacgc gaatatcatg agcgaaatat
     2341 ttacccccaa attgcgcacc aataccaaat tgctgactgg cctcgagcaa cactttttcc
     2401 agctcaatat cacggaatgc ttgtccctgc tcgtttccgc tggtgggcaa attgtcgtaa
     2461 tatttcgttg aggccagctt ggcaattttt aaggtttgat cggctgaaag ccctccgacg
     2521 acaaaggcga tgtggtaagg cgggcaggca gcggtgccca gcgatttcat tttttcgatt
     2581 aaaaatgcgg tcagtttttc tggttgcaat aaggatttgg tttcctgata aagcgcggct
     2641 ttattggcgg agccgccccc tttattaaca cagagaaaat ggtactcatc accagcgaca
     2701 gcgctgatat caatctgcgc cgggagattg gtttgcgtat tgacttcggt atacatatcc
     2761 agcggcgcat tttgtgaaaa gcgcagatta ttttcctgga aagtactgta aatgccttta
     2821 cttaaggctt cggcgtcgtt acctccggtc catatctgtt gccctttact ggcaacaata
     2881 gttgcggtgc cggtatcctg acagttgggt aatacccctt ttgctgagac ttcagcatta
     2941 cgtaataact gcaacgcaac gtatttatca ttactgctgg cctgctgatc gttaagaatg
     3001 ctggcgacct gttgcaaatg agcggaacgt aagaaaaaag aggcttcata aaatgcctgc
     3061 cgggcaagca gcgttaatgc ttcaggagcg atttttatca cttcttcgcc gtctaattcc
     3121 gttactgtaa tatgttgatc gctaattaat gtatattctg taccgtcttt attttgtaga
     3181 aacggttctt gccagataaa tggtttggac atatttcact tttcacctta aacgttagtg
     3241 ttacttaatt gccgtctaac ttcatgatcc ggcttataaa aatgcgtagt cattggcttc
     3301 tgattgctta agatccccct cagggcagag caacgttcga ttcattgggt tttaatgctt
     3361 taccccgaca gccctcgccg aaatgttcgc cttcccaccg actcaccatc gcagcgccca
     3421 ttgcgttgct catgacgttt gtcgcggaac gccccatatc aaggaacgga tcaacgccca
     3481 tcagcaagat cagccccgct tccggaatat tgaactggtt gagcgtggcg gcgataacca
     3541 ccattgaggc gcgtggtacg ccagccattc ctttcgaggt caacatcagg atcaacagca
     3601 tggtgatttg ctcaccgatg gataaatgga tattgcaggc ctgggcgatg aaaactgtgg
     3661 cgaaggagca gtaggccatt gatccaacga gattaaatga gtagccaatg ggtaagacaa
     3721 agctggcaat tttgggggaa acgccaaatt gctccagttt ttcaagcgtt cccggaaaag
     3781 ccgcttcaga actggatgtg gtaaatgcca gcagggcggg ttccgaaagg gcgcgggtca
     3841 ggcgtctgat gcaggggccg acataaacga tggccagacc gataagcagt acccaaagta
     3901 ataacatggt gaaataaaat tcacccataa agatcccggc gctcaccata actgccagtc
     3961 ctcgttcagc aatcaatgct gaaatagcag cgaatacggt caggggagcg aagagcatga
     4021 cgtagccagt gagctttaac atggcatgtg ccagcgaatc taaggcgtga acgatggcgc
     4081 tgcctttctc gccaatcgcc gtcaggctac agccgaggaa aattgagaac accacgattt
     4141 gcaaaatttc attgtgcgcc atggcatcga caatgctggt ggggaaagca tgcgaaataa
     4201 atacttttag cgtaaagggt tctgccgcga ccactccggt ggtttcggca ccgtgtgcaa
     4261 caaaattaat tcctgtacct ggcatgaaga aatttaccgt tatcaagcct aaggcaattg
     4321 acagcaatga ggcgcaaata aataaaaaga gtgttttaga aaaaatacga ccaagggctt
     4381 tggcatcgcc cattttagca atacctacca ccagggtaga gaccactaac ggagcgataa
     4441 tcatttttat cagtcgtaag aaaatcgtcg tgaatatcga tatttcttgc gcatagagtt
     4501 ttgcggtttc cggtgaagca gtgttattga gcactacgcc gataatcatt ccaagtacca
     4561 gcgccaaaag aatcatcgtg gttaaactta ttttcttcat tttttcctcc atcaggtgca
     4621 cggcgataaa tcagaatcag gagaagtaga tgcctgaata gagattcagg catctacctg
     4681 ctattacaac cttacaaata gctgaagtgt tgaactgcaa taagtgacgg gatgtcagtt
     4741 ggctaaaagt attcggattt ttacaaatcg aaactcatat ttaataaagg tgcatgttgc
     4801 tgtgcaccat agacatcatt gtcacctaca ttcccggaga taatatctcg tggcatgacg
     4861 atttttactg cgttagaagg atcaaaccag acaatacgat gaataaagtc aggttcaaca
     4921 tgatataagc gtgcaatcag ttctggggtt aattgccctg atgatttaac tcgctcatag
     4981 ttttctttcg ttttaaataa aatatctaaa accagttcgt aaggtccggc atttttcgaa
     5041 cgaatgactt gcgccagtga gacaattgac tgtttcatgc ctgaactcct tctggcgtca
     5101 cctgttcaat atggaaatca aaacgcagag cgtcgttggc ttcaatcagg tgatatatgg
     5161 aaaattcgta taccggcccg ccctggatat cagaagggga gaacgggaac gccagattac
     5221 ctgcggtagc gatgcggttt tcatagccgt agtgcagcat ggtagagcgc accagcgagc
     5281 aaacgctgtt ggcaatttcc tgggtcggtg caactacatc aaggacaatc cccagctcat
     5341 gcccggcagt ttgcatcggt tcatggtctc ccatcacccc gtttttgccg tacaggtgga
     5401 aattgatgcg aatgctgtca tcgtcgagcg acaggttacg cgaaacgctg gttttgactt
     5461 catcgatgat tttatcgatc ccggcgatca taatcgggtc gcgcgttccg gcgatggtca
     5521 gacagcggaa gcccacccgt cgtgcacctt ccagtttcag ggcatacggc gtttcttcat
     5581 gcttagaacc gctgacgtag acttcgccgt catttactgc tttaaatgtg cagcctttca
     5641 ggttcaacac gccgccaggt cctggcagga agtagggatc ggatttctca tacagtgtgt
     5701 gtgcagctgc tgacgtttcg gtaaatttac gcttcggatt aaatgttttc agcgtaaagc
     5761 cgttgtcatc aatgatgccc atcgcacagt cagagcctga gccgggcgtt gcggcgattg
     5821 ccgcgcattc aaggattttc ccacaatgca gcgccagacc ttcatcaaag ccttgcatga
     5881 tcgggagcgc agcaaagcag gccgggtcgt aagcgcgccc gcccagcacc acttgcgcgc
     5941 gttctttcag cgcccgctgg aagggttcga tgcccatttg cgcgacgata tacgtacttt
     6001 cgtcaatcgc gtcgtgggtt aacggcggga caaaatccag cgccgtgatt ttgccgttat
     6061 ccagcgcctg gtggacgatg gctttgtcaa cgtctgccgg gatcaacgcc atcgagaacg
     6121 agaggtgctc ttcccgggca atctcatgaa ttatttctcg gcaccactcc aggtgtggtg
     6181 cggcaccaga accgccagcc gttccgatca ccaccggtat attattttgc acgcctgcgg
     6241 tgatcatata gcgcagatcg cgcttgaccc ctgcgcgatc ggtaaacggt ttgcctgccc
     6301 cgaggtagtg ggggcctgga tcggaagagc ctgcatcaac ggcgatcaga tcgggcgatt
     6361 ctgccatcgc tttgcggaag ctctcttccg ggaatccata cccgaggatt gccgttggcg
     6421 ataagacttt aaaagaacga gccatttgtt aatccttagc ctgcaacagc gcgatagtac
     6481 gattcatttc gttgaagacg atatcgaggc cttcgtcaaa gcccatccct ggttttacca
     6541 gcatacgcat ggggcgagcg gcaagggcga cgtggacaca ggtgcgggca ctgacatcag
     6601 tttcattgca ggtgccgccc tggtacgctt ccatgctgtg gctgttgcag taaagaaccg
     6661 catcgacgat gttgtgaata ctgcccagat ccggggtttt gatttgcacc atgtgacaac
     6721 tggcagcatc agtgaaatca acaatatcct ggtaggtgtt acaccattca tcggccacaa
     6781 ttttcacgcc ggaaccgagg cgcgtcagct ctttggtaat cgcggtcagc aggcgaattt
     6841 gatcgggctt gttaccggca tcgaccggcc cttcgatgta gagcggcagg ccttgcgctt
     6901 ctttttccag gctggcgatg tattgcgcac agcgaagcgg atccatatcg aagatcagac
     6961 cgatagtgcc gtatacatcg atgtgcaggg tagggtggta gcgtgcgctg gtgcgcttgc
     7021 ttagaatgcg atccgacaac cagcggacat attcgcgcag tttttcacct ttaaagccca
     7081 gcttctcttc gacgttatta atcagcgcat ggggcagcac gtcgatgcct ttaaggatca
     7141 tcttatcgac ggcgatatat cgatcgtcgc cgctctgacc aaataatgga atggattccg
     7201 ccacgcgtgg caactgccat tcatcacaga ccacttcagt tttcagacgg ccggttgcca
     7261 gcgcggtagc atcaagcagc gcctgtgata atccgtagcg cacggcggta tgcagcaagt
     7321 tgccgtcaat acgcaatttg tcgaagaaac gggcattcgg caggaaagca tccacatcgc
     7381 ggcctaccag taatggcttg atatggtcgt tgaggaacgg aataaagtgc tctgcgagga
     7441 acagtggatc gcgaccgcct gccccggaat actgtacggc agtacagtca cctacggcga
     7501 ccgcgccgtt ttccagaatc aactgtaccg atacgcactc cccggcctga cgtacggcgt
     7561 taaacccctg tgttactggc tccccggtat aaaaaaagcc gtcatgaccc gctccgtttt
     7621 ttatcgcctg ctggtcatcg aaatagaatg aggagtagcc agcggtgaac agagcctgtt
     7681 ttattttcat tatggtcttc ctattaattt actgtgggat acagcattga tgtcatctac
     7741 aaccatctgg aatgtcggtt ggcgtccttc acagcgtgcg cgttccgcaa cgtaatcgtg
     7801 gtggaggtcg agaatatctt ttggcaatgg aacggctccg gcttccagta cgcgtatcgc
     7861 gccggtgttg tcacggaccg gaagaatttt gcccgcgttg caggcggctg gcgcaaacgg
     7921 cacatccagt acgcccgctt caaatgccag caccgttccg cgcgcaatat caccattacc
     7981 cagttcgaac actttattca gtaccgcgcg aacctcgctc ttaatcagtt caatttccag
     8041 ttcaaccgca gggcacggcg ggaatttctg ctcattgacc atgttgagca tttggcggga
     8101 ggctttcagc ccctgaatgt tggcagcggc agtcgggata ccccaggctt cgtgcggact
     8161 tttggtgatg actttggtgg caccagacat ccccgcgacg gctgcgcccc aggagataat
     8221 agcgaacgct ttcgattcgt cttccggaaa gccgcccatc cactggtgaa acacggtact
     8281 caattcatag tcggtatagc catagctctg aaaatattcg tgtgcaagtt cacgcagaga
     8341 ctggatcgcc gcaacatcct gcgtcaggct gccgacctgg ccataaccaa cggtgataga
     8401 tttcacaccc tgttccagcg ccagcaagcc ttcgattatc gcgatagagt gcgagataaa
     8461 cggcgggatc agcgtacccg ttagtgggcc aaacggttcg cggttgatgc ggatcccgtg
     8521 ttcttcatac atccccatta aacggtcgca gtactgccag tcgcggatcg atttttccag
     8581 cgttacgcgt ttggcatacg gaatgttgta ggaaatgcca ccgccttcat aactggtaaa
     8641 gccgctggcc attgagattt cagccagcag acgcgcatct ggcgtgccgt ggcggatctg
     8701 tagcggtttt tgcagggttt cggtgaggcg acggcaagct gcgacaccat ggttaactac
     8761 cggtaaaccg ttcagtttag aggttccggc ttcgatggat tttttaatcc ctacagccgc
     8821 ttcttcgtac cgattcagac gggtatatgc atcgatggtg cttggcagca gatcgcactc
     8881 ttcttgtagt gttttcagca gttcgatgtg ttcgtccatc agcgccacac ctgcgcgcgg
     8941 ttggctaagg gttttacctt ccttgtctgc tttcagcagc gcaagagaga aacgtttatg
     9001 ctcagggatg gtttgttggt atttaacgcc atcttcaaag ttttcaacat ccttgccggt
     9061 ttcccaggtt ttcagtacct gttgccgctc ggtcatgaat tcgtcatggg ttaatttctt
     9121 atttcgaagt tccattgatt gcagccttaa ttgttatagg gttaagcatt gaatactggt
     9181 gcgcgcggcg gccagcggat cgactctggc gacgtttgcc agcagtggta atagcccccg
     9241 ggaatcgcgg taatattcaa attcggtcgg taagagaatg cgtttaccgt cgtcgtttaa
     9301 ctcgcggtat ttcaaccagt gatgcatatc gaactgactg gcacgcgaga gccatccgcc
     9361 ggatccgacg actttacgca cggttgtcag gtcacgtccc atctgtaaat cgacattgcc
     9421 gacgcaggtg cagacctctt ttttggtgcc cgcatgtctt tcggtggcat aacccacaca
     9481 cagtccggcc aggacggtat cgaaatattt ctcttctgca tttgcgggta agtagtcggg
     9541 atgagcgact agatgacgga ggtaatgata aaaagcgtcc agctgtgcgg gttgctgagc
     9601 aaaattcaca ttgactaact ctttcgcact ttcaccaacc accactgccg agacgcgcat
     9661 tcccagatcg ccttccaccg tccgttttac gaagggttcc ggtacgccgt gtaaaacggt
     9721 gtcgggcgag agcgtgttgg cacaggcgga ataaacatcg gtggttgcgc cgcccatgtc
     9781 gatcagcatg aactctttcc agctgtgatc gacgttactg attgccttga ccagttcaaa
     9841 gaccgtccat ggtgtcggca ttggttcttc gcctgtttta tcaacaatga catccagccc
     9901 tttcccttta acgatgcgtt taaggaatat gtcgcaaata gcctggcgtg cagctacagg
     9961 gttcggatgg tcaagatcgg gcagaacgtt gtcgacaatg gtcaggtctt tatgcccgag
    10021 gatctcctgc acctcgtcct gaatatcccg gtttccggca taaataatgg cgcagtcgag
    10081 tttggattct gccagcacac gggcgttatt cagtccgtag ctttcttctc cgccgtcggt
    10141 gccgccggtg aacaagagaa tatcgggctg cgtttcttcc agtgcctgga tatcacggcg
    10201 gttcagcttg taggcgtaat attgcgcaat tttagcaccc gcagagtgag cggttacttt
    10261 cgcggtttcc agggtgatag aaggcaccaa tcccattgcg gcaaccgcca gcccaccttt
    10321 ggcagaggag gaatacttca atgcaacctc accgctgctt aacaggggga gggcattatc
    10381 tacgtttaac acctggttca ggctggagaa aaaccccttc gccagatgat gggtggtggt
    10441 tggggtcagt acgtggttaa ccaacgttaa cgcgtccccc tcccgggtaa agagggctgc
    10501 tttggtccac gtcgatccaa tatcgacaga gacgatttgc atcagatagc ctcttcaaga
    10561 atacgagtat caacgtcatg acgttggttg atatcgtggg ccatcagctg gcaaacatct
    10621 tctaagtcat gacttggggc aaagacgcga tcaaatccca tctctttgaa tttggtttcc
    10681 acgtcagcga agtcatgttt tccgaccacc aggttaccgc ccacatagag caggatgtcc
    10741 cccagtccac gctcgatgca gcgttcgcgc atgcccagac agtcgatatc gccatgccca
    10801 tagatagagg agacgacaat cgcgtcagcg ccagtttcaa tggcagcatc aatatattcg
    10861 tcctggctga ccattacgcc cagattaatg actctgaagt cgtggttgct aaaaacgcgg
    10921 tccagaactt tattgcctac tgcatggcag tccgcgccaa taacgccaat cacaagtgtt
    10981 gctttcttca tgggtaatcc tttgtaagac agagagtaaa ataagttaca cacgtcgtat
    11041 ttatggccgt tcataacggc acaggtttaa tatcacagga gtgtctttat ctgaccgtaa
    11101 tgttgcaatt gatctatttt caaaatgtct ttcgggaaat gattgtttga agt
//
LOCUS       DAZKFZ010000149        11090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000149 DAZKFZ010000000
VERSION     DAZKFZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(70..666)
                     /gene="nrfG"
                     /locus_tag="ACHOQW_003431"
     CDS             complement(70..666)
                     /gene="nrfG"
                     /locus_tag="ACHOQW_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP9574455.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(663..1046)
                     /gene="nrfF"
                     /locus_tag="ACHOQW_003432"
     CDS             complement(663..1046)
                     /gene="nrfF"
                     /locus_tag="ACHOQW_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP9574456.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(1039..2757)
                     /gene="nrfE"
                     /locus_tag="ACHOQW_003433"
     CDS             complement(1039..2757)
                     /gene="nrfE"
                     /locus_tag="ACHOQW_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP9574457.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            complement(2777..3733)
                     /gene="nrfD"
                     /locus_tag="ACHOQW_003434"
     CDS             complement(2777..3733)
                     /gene="nrfD"
                     /locus_tag="ACHOQW_003434"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP9574458.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3730..4401)
                     /gene="nrfC"
                     /locus_tag="ACHOQW_003435"
     CDS             complement(3730..4401)
                     /gene="nrfC"
                     /locus_tag="ACHOQW_003435"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP9574459.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4398..4964)
                     /gene="nrfB"
                     /locus_tag="ACHOQW_003436"
     CDS             complement(4398..4964)
                     /gene="nrfB"
                     /locus_tag="ACHOQW_003436"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP9574460.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(5009..6445)
                     /gene="nrfA"
                     /locus_tag="ACHOQW_003437"
     CDS             complement(5009..6445)
                     /gene="nrfA"
                     /locus_tag="ACHOQW_003437"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP9574461.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6838..8796
                     /gene="acs"
                     /locus_tag="ACHOQW_003438"
     CDS             6838..8796
                     /gene="acs"
                     /locus_tag="ACHOQW_003438"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP9574462.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            8997..9311
                     /gene="yjcH"
                     /locus_tag="ACHOQW_003439"
     CDS             8997..9311
                     /gene="yjcH"
                     /locus_tag="ACHOQW_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP9574463.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9308..10957
                     /gene="actP"
                     /locus_tag="ACHOQW_003440"
     CDS             9308..10957
                     /gene="actP"
                     /locus_tag="ACHOQW_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP9574464.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         2587 a         3098 c         2922 g         2483 t
ORIGIN      
        1 tatttttggc atatgttcat tcgattattt tttatgcagc tcaataacat aatcttcatc
       61 accagatgat taatccgatc tccgctgcaa caatttcgcc atattaatcg actcgaccag
      121 ttgtgtccgg ttgatccggg gtgagttgag atccatcact ttttgccata gttcgatggc
      181 ttgcgcgtaa ttcgcctgca taaacgcatc ggaagccagc agcatcagag cggtgatttc
      241 gttagagtcc agcgcgaggg ctttgtcgat cattgcgcga gtctgggcgg tcatatgctg
      301 gctggcctgg taataaagca ccgtcgccag cgccgcatac agttcagcgt tctcaccacg
      361 tagttgcaac gcctgacggt acgccagcag cgaattgctg taatcgtttt gccacagata
      421 gtactcgccc agtaacgccc actgttcgct gttttgtgga ttagcgcgga ttttatcctg
      481 caatgcctga agctgcgctt ccgggttttg ctggctggca aactgatgta gcggatcgcg
      541 ctgacgctga tactccgcac gtaccgcctg ccattttggg cttaacagat aactaccgat
      601 acagaggaat accatcaata tcgtgagtgt tgtcagcatt ttcaccggta tttgaggctg
      661 tttcatcgct tcgccctcac tcgccagagg atcagtgcca tcagcagtaa caacaccact
      721 ggcagcgccc acaacaccag cgtctgaccc gttaacggtg ggttatagcg aacaaaatct
      781 ccgtagcgtt cggtcatcca gccaatgatt tcgacttcac ttttcccctc cgccaccatg
      841 ctgtaaacct gatggcgcat actgactgcc accggcgcgt tggattccag taagttttga
      901 ttctggcact gcggacaacg taactggctg gcaatattta acgcctgttg ctgttgttgc
      961 ggattggcga attgccaggt gtctacaacc tgagcgcgcg caaaacaggt aaataacagc
     1021 agtagcgtga gaagcccttt atacatcgcg cttcttcccc cgccatccgc ttaacaatgc
     1081 gcccgcaatc atcaacaatc ctccccccca gatccagcgc acaccgcttt gtacatacaa
     1141 gcgaaaagcg taacgatccg ctccagtttt ctcccccatg accgcatacc agtcatggat
     1201 gccgttccag cgaattgacg gttccatcat ttgctgacga cgagcttcgt aaaaacgccg
     1261 ctccggcgtt aattcaccaa tgcgttgctg atggtcaaac agcgccacta tcgctttttc
     1321 gctggtgtaa ttgcctttgg cttgcagatc gaggcgctca aaacggaagg tgtatcctgc
     1381 cagcgtcacc tgctgacccg gctgtaaatt caggctgatc tcctgacggc tgacgctgga
     1441 gacaacgatc cctgcggcaa ataacagtat gcccgcatgc gccagcagcg ccggaagctg
     1501 cgcgcgtttg ccagagacaa acgtcgccag cacaatcacc accaacatca acagaccaaa
     1561 cggtaacgtc gcgcggttaa aatacggtgc gccaacggag agacgtcccc agcccagcag
     1621 gccgtaaatc atcggataaa gcgttcccac cagcacgatc agtagcactg cgcaaaacag
     1681 caacagcgta gcgaggatta acatttcccg cgataacccc gaaaaacgca ccgccgggcc
     1741 accgtcccgc gctcgccagc catacagagc cagagacgca aggctaatca gtgcaaacag
     1801 gctgaacaac ggcacagcgc gaacgttatc cagagcgaac gcatgaaccg aaaccagaat
     1861 gccagaacgg acaattaagg tgcccagcag cgacagcatc agagtaacta tcgccaacaa
     1921 cagcgaccag tggcgaaaaa tcccccgctg gcgtgtcagg gataaactgt gcagcagcgc
     1981 agtggcagaa agccagggta ataaagaggc gttttccacc ggatcccaga accaccagcc
     2041 gccccagccg agttcgcagt aggcccacca ggaaccgagg atgatccccg cagttaatgc
     2101 actccagcca ggtaacgccc agcgccagca aattcgggcg caggcaccat caaactcgcc
     2161 gcgcagtaga ctcgccagcg ccacgctcgc cgctaccatc aaaccgccat agccaaggta
     2221 aagcagcggt ggatgaaaga taagaccggg atgttgcagc atcggattga gatcgcggcc
     2281 ttcgattgct ggtggaaata tccgcacgaa gggatcggac cacagcacta caaacagtag
     2341 cagtgcggcg agcatgagag ataaaacggc tagcgtcagc ggaaatagcg gatcggtttg
     2401 ctgccgatat tgccaggcaa acagcgcgct ccaggcggaa agcagcagca cccaaagcag
     2461 cagcgaacct tcatgaccgc cccacaccgc cgccagtttg agtttccacg acagcaagct
     2521 gtagctatgt tgggcgacat aaatgaccga gaaatcgctg atgagaaaac aatacgtcag
     2581 tacgccaaag gcgagcagca ggagcgcgaa ctgtgccaga atgccgatgc aagtgagtcg
     2641 catcatggca ggccagcgca accgcactcc cgcaaaggcc gtcaacgggg tcaacacgtt
     2701 gaccccgaga cttaacaaca gcgccagaaa accgacttca ggaaggaata cgtccagaaa
     2761 ccacctctgt tctggcttac gccaccgtta actgcccggc gtagagaatg aaaaatcgca
     2821 acatcaacac gccgaccaga ctggccccac acgccgccag cacggcagga atgccggaac
     2881 tgcgattgac ccacggtttg aacaacattg gcacaatcag ccccagcccg gcgacaccaa
     2941 gccagaacca ccacgtccag aaaccgccgc ccaatgccgc caccagcgca cgcactttac
     3001 cgtcatcgcc cagcgccaga ccgacaaaaa acgccgccag caggaagatt tcaccccata
     3061 ccacggggat ttccatacgg tgtacaaact gcgcttccgt ggaatgcggg ttactgcgtt
     3121 ggcgtatcgc catggcgatc agtgccaccg ccgcaccgga cgagatgccg gagaagagga
     3181 acagcaccgg caggatcggg ttattaagga acggatacga tttcagcgcc gacagcagaa
     3241 aaccggtata agcccccaac aacaccgcca gcaccagcat caatgtttcc agtccgcgat
     3301 gaacgggcgt cagcaggctc agaacctttt gcacgatccc cagcttcggc aaccagcgtt
     3361 gttgcagggc aagcaaatca tgttcaaaga ttttcgccag ccacagcacc agcaccacca
     3421 tgtaaagctg aaacagcatc acccccatcg acatcaccga agtaaaactg tagtggaaca
     3481 tcagcttcca gaaggtccac ggtcttgtca ggtggaagac gaggctcaac agaccgagga
     3541 tcaccgcgcc aggtccgaca atcagcgtgg tgcgcagcaa cgtgctgtct gcaccgcccg
     3601 cctgcgggta gaagcgacgt aacagcacgg ccagcgtcac cagaccggca gaaataccaa
     3661 tcaaaaacag gtagatggca atcggccagt cccacaccag cgattcaaaa tgaaatgcgg
     3721 aagtctgcgt cattggctca cctccccgta tttaaacggt acgcggtaca gcttcggttt
     3781 ggttcccagc gccagcttgt agcggtaagt gggcttctgg cgcagcagtt gcgaaatctc
     3841 actgttggga tcgtccagat tgccaaacgt cagcgccttc gtcgggcaag cctcaacgca
     3901 cgcgggaagc ttaccggcct gcaaattggt cttacggcag aaatcgcatt tgtccgctgt
     3961 cttcgtgacc ggatgaataa agcgcacgcg gtacggacag gcggcgatgc agtactgaca
     4021 accgacgcag agatccggat taacatcgac aatcccactg gcagcatcgc gaaaagatgc
     4081 accggtcggg cagacgtcaa cgcacggcgc atgatcgcag tgctggcaag acttacggaa
     4141 gaagcgatat ttcacatcag gaaattcgcc ctgcggctcg ctacgaataa tcgtcaggcg
     4201 cgagacgcct tccggcacct tgttcacttc ccgacaagca tccatacagg cggtacagcc
     4261 gatgcataac gactcgtcat gcaccatgcc gtaacgcacc ccattgatat tcaacgtttt
     4321 cgccaccacg cgccctgcgg tgccgctgac ggctgccagc acgccgacgc cggtgagaaa
     4381 ctggcgacga gaccaggtca tggctgctcc ttaagcaacg gaacagacgc cgggttaaag
     4441 ttcggattgg tgcgctgatc gctgtggcaa tcgacgcaaa tcttaatccg tcctttgtca
     4501 cttaacgtct gcatcgtatc ttgctgtgga tgcagggaat ggcagctggc gcacgccact
     4561 ttggttacgt ggacatcgtg cggccagaac gctttttgca actgttccgg cagatggcag
     4621 gacatacaga cgctgttctg ctctccaacc ttgtacatcg gctcgttaaa gcgcatcaca
     4681 tctttcaccc cttcgcggtg ttgcggtgat ggctggccgt gacagttggt gcaggtgacc
     4741 ggcagtttgt tattcgggtt gataacggaa gcatgtttgc catgcatacc ttcggtatct
     4801 ggcttatgac agtccagaca ggcggcatcc gggttacgct gctgggtaac ttcgtagcga
     4861 tcatcagatg cctgcgccgc aggggtagcg gtaattccgt tcaggctcca caacaggccc
     4921 gacgccagca ccccggcagt taataacgaa cgtaatacgc tcatattcac tccattgtgt
     4981 tttccgcccc ttgcgaggcg gaacggggtt attggcttaa cagaccgttt ttacgtgcct
     5041 gctcttccca ctgcgggatc accgttttga tgaagtcctg cttctcggcc ttgatttgtt
     5101 ccatgttcag gccaatggcc tgctgggctt tctctttggt cgagatatcc gggatctgga
     5161 tttcatgggt gatgccttta gtcgccagca ggcgcgccag tttggtgcgt gcatccgccg
     5221 ctttatccat cgccgtaccg agcatcctca gaccttcttc cggtgcgtgc atatgaatgc
     5281 cgtgggaagc gatcgccaga tcccaacgcc actgggcatg acggatatcg tcctgaatcg
     5341 gcttcatttc ggcttccgtc gcacctgcgt ccagcgccgc tttcgcttcg aagtgagcgt
     5401 gaaccagttg atcttcaacc ttgattttca ggtcgttaat cgactgctta cgttccgcga
     5461 ccactttttg caaggcagct ttgtcctggg tatggcagtt cgcacaagtc tgggcgaagt
     5521 tatcaaacgg attaccaatt ttatggtcgg tgtagagttt gccttcggcg ttctgcactt
     5581 ttggcatatg gcagtcgata caggtcacgt tgtttttacc gtgaatgccc gctgtccagg
     5641 tttcatattc cgggtgctgc gctttcagca ttggcgtttt cgacagggag ttagtccagt
     5701 cagagaaggc aattttgtcg taatactgct ccatattttc gactttcatg ccgtcatccc
     5761 acgggaattt aaccgctttg tttttgccgt cgaagtaata ctccacatgg cactgaccgc
     5821 aaaccatcga ttgctggtcg aaacgtccgg ctttctcaaa aggtttacca atggcttcca
     5881 tcgcgcgagc cgcatacgga cgggaaaggg ttaactccgg tttgcctttg gcgaactctg
     5941 gagaggcggt gttatggcaa tcggcacaac ctaagttgtt gacgatttcc gggccgccgc
     6001 gcgcccattt accgtggaag tagccatctt cgccgtcttt ctggatcaga cgcgccacat
     6061 ccgggctttt acaactccag cacgccatcg gtagcggacc atcttcagcg tttttcggcg
     6121 caccggtacg cagggtttca cgcacatcgg tcacagcaaa ggcatgtcca cgcggcttgt
     6181 tgtaatcgcg cgagaaggga taccccgccc acaggatcac cagccgtgga tcttccgcca
     6241 gggcgtcaac acgctctgac tgttccgagg tggctttcca ggagagatat tgatcgggat
     6301 gctgcggggc aaaggtttca ttcttcgctt ccacagttac aggttttgcg ggagcagccg
     6361 tttgttcagc gtgaacagaa gtgaaaaaga aaaaaggaat caataagctg aagatacggc
     6421 gtgcgtttat ttttatcctt gtcatagggg cttcatccga attgcgccat tgttgcaatg
     6481 gcggttttta ttgtttttca cgacagtaac cgcacctaca ctgtcatgac attgctcgcc
     6541 cctaagtgta acaaataacc acactgtgaa tgtcgtcttt aatcaattgt aagtgcatgt
     6601 aaaataccac tttagagtta gtcagtatct tcctcttttt caacagcatg cataactgca
     6661 tgttcctcaa agaattaatc aacttttgtt gctgaccttc aaagattacc ctgccgttta
     6721 tttgcacaat tctacttttg cgtgatctgt cgcccaaata ctaaacaaaa ctgccaatac
     6781 ccctacattt aacgcttatg ccacatatta ttaacatcct acaaggagaa caaaagcatg
     6841 agtcaaattc acaaacacac cattcctgcc aacatcgcag accgttgcct gataaaccct
     6901 cagcagtacg aggcgatgta tcaacaatct attaacgcac ctgatacctt ctggggcgaa
     6961 cagggaaaaa ttctcgactg gatcaaaccg taccagaagg tgaaaaacac ctcctttgcc
     7021 cccggtaatg tgtccattaa atggtacgag gacggcacgc tgaatctggc ggcaaactgc
     7081 cttgaccgcc atctgcaaga aaacggcgat cgtaccgcca tcatctggga aggcgacgac
     7141 gccagccaga gcaaacatat cagctataaa gagctgcacc gcgacgtctg ccgcttcgcc
     7201 aataccctgc tcaagctggg cattaaaaaa ggtgatgtgg tggcgattta tatgccgatg
     7261 gtgccggaag ccgcggttgc gatgctggcc tgcgcccgta ttggcgcggt gcattcggta
     7321 attttcggtg gcttctcgcc ggaagcggtt gccgggcgca ttatcgattc caactcacga
     7381 ctggtgatca cttccgacga aggcgtgcgc gccgggcgta gtattccgct gaagaaaaac
     7441 gttgatgacg cactaaaaaa cccgaacgtc accagcgtag agcatgtggt ggtactgaag
     7501 cgtactggcg ggaaaattga ctggcaggaa gggcgcgacc tgtggtggca cgaccaggtt
     7561 gagcaagcca gcgatcagca ccaggcggaa gagatgaacg ccgaagatcc gctgtttatt
     7621 ctctatacct ccggttctac cggaaaacca aaaggcgtac tgcacactac cggcggttat
     7681 ctggtgtacg cggcgctgac ctttaaatat gtctttgatt atcatccggg cgatatctac
     7741 tggtgcaccg ccgatgtggg ctgggtgacc ggacacagtt atttgctgta cggcccgctg
     7801 gcctgcggcg cgaccacgct gatgtttgaa ggcgtaccga actggccgac gcctgcccgt
     7861 atggcacagg tggtggacaa gcatcaggtc aatattctct ataccgcgcc cacggcgatt
     7921 cgcgcgctga tggcggaagg cgataaagcg atcgaaggca ccgaccgttc gtcgctgcgc
     7981 attctcggtt ccgtgggcga gccaattaac ccggaagcgt gggagtggta ctggaaaaaa
     8041 atcggcaacg agaaatgtcc ggtggtcgat acctggtggc agaccgaaac cggcggtttc
     8101 atgatcaccc cgctgcctgg cgctaccgag ctgaaagccg gttcggcaac acgtccgttc
     8161 ttcggcgtgc aaccggcgct ggtcgataac gaaggtaacc cgctggaagg ggctaccgaa
     8221 ggtagcctgg tgatcaccga ctcctggccg ggtcaggcgc gtacgctgtt tggcgatcac
     8281 gaacgttttg agcagaccta tttttccacc ttcaaaaata tgtatttcag cggcgacggc
     8341 gcgcgtcgtg atgaagatag ctattactgg atcaccgggc gtgtggacga tgtgctgaac
     8401 gtctccggtc accgtctggg aacggcggag attgagtcgg cgctggtggc gcatccgaaa
     8461 atcgccgaag ccgctgtcgt cggtattccg cacaatatta aaggtcaggc gatctacgcc
     8521 tacgtcacgc ttaatcacgg ggaggaaccg tcaccagaac tgtacgcaga agtccgcaac
     8581 tgggtgcgta aagagattgg cccgctggcg acgccagacg tgctgcactg gaccgactcc
     8641 ctgcctaaaa cccgctccgg caaaattatg cgccgtattc tgcgcaaaat tgcggcgggc
     8701 gataccagca acctgggcga tacctcgacg cttgccgatc ctggcgtagt cgagaagctg
     8761 cttgaagaga agcaggctat cgcgatgcca tcgtaaccca caattgccgg atgcgagtcg
     8821 gtaacggttt gtaggcctga taagacgcga cagcgtcgca tcaggcattg attgccggat
     8881 gcggcgtata acgccttatc cggcctacat tcggcaaggg ttacccgagc gttaaccttc
     8941 tcccataagg gagcgggaat taaaacaatc cctacattac ctctggagaa tctgtgatga
     9001 atggcactat ttatcagcgg atagaagaca atgcgcattt cagggagtta gtcgaaaaac
     9061 ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc agtttatatc ggctttattt
     9121 tactgatcgc cttcgcgccc ggctggctgg gcaccccgct gaatccgaac accagcgtca
     9181 cacgcggtat tccagtcggt gttggggtaa tagtgatctc ctttgttctc actggtatct
     9241 acatctggcg ggcgaacggc gaattcgacc gtcttaataa tgaagtcctg catgaggtac
     9301 aagcatcatg aaaagagttc tgacggcgct tgccgccaca ctccctttcg cagctaacgc
     9361 cgcggatgct attagcgggg ccgtagagcg ccagccaacg aactggcagg cgattattat
     9421 gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac tgggcgtcaa aacgcgtacg
     9481 ttcgcgtaat gactattaca ccgcaggcgg caatatcacc ggttttcaga acgggctggc
     9541 gattgccggt gactatatgt ccgccgcctc atttttgggg atctccgcac tggtgtttac
     9601 ctccggctat gacggcttaa tttactcgct gggcttcctg gtgggctggc cgatcatttt
     9661 gttcctgatt gccgaacgtc tgcgtaacct ggggcgttac acctttgccg atgtagcctc
     9721 ttatcgtctg aaacaagggc cgattcgtat tctttcggcc tgtggttctc tggtggtggt
     9781 ggcgctttac cttatcgccc agatggtggg tgcaggtaaa ctgatcgagc tgctgtttgg
     9841 ccttaactat cacattgcag tggtgctggt cggcgtgctg atgatgatgt acgtcctgtt
     9901 cggcggcatg ctggcgacca cctgggtaca gattatcaaa gctgtgctgt tgctgttcgg
     9961 tgccagcttt atggccttta tggtgatgaa acacgtcggc tttagcttca acaatctgtt
    10021 cagcgaagcg atggcggtac acccgaaagg tgtcgacatc atgaaaccgg gcggactggt
    10081 gaaagatccg atctccgcac tctctctggg gctaggactg atgtttggta cggctggctt
    10141 gccgcacatt ctgatgcgct tctttacggt cagcgatgcc cgcgaagcgc gtaaaagcgt
    10201 gttctacgct accggattta tgggctactt ctatatcctg acctttatta tcggcttcgg
    10261 cgcgatcatg ctggtcggtg cgaatccgga atataaagac gcggcgggcc atctgattgg
    10321 tggtaacaac atggcagcag ttcacctggc caatgcggtg ggcggcaacc tgttcctcgg
    10381 ctttatttca gcggttgctt tcgccactat cctcgcggtg gttgcgggtc tgacgctggc
    10441 gggcgcatca gcggtttcgc atgacctgta cgctaacgtc ttcaaaaaag gcgcgaccga
    10501 acgtgaagag ctgcgggtat cgaaaatcac cgtcctgatt ttgggtgtga ttgcgattat
    10561 cctcggcgtg ctgtttgaga atcagaacat cgcctttatg gtggggctgg cgtttgccat
    10621 cgcggcgagc tgtaacttcc cgatcattct gctttctatg tactggtcga aactgaccac
    10681 gcgtggcgcg atgatgggtg gctggctggg gctgattacc gcagtggtac tgatgatcct
    10741 cggcccgacg atttgggtac agatcctcgg tcacgaaaaa gccatcttcc cgtatgaata
    10801 cccggcgctg ttctctatca ccgtggcatt cctcggcatc tggttcttct cggcaaccga
    10861 taactcagcg gaaggcgcgc gtgagcgtga actgttccgc gcgcagttta tccgctccca
    10921 gaccggcttt ggcgttgagc aaggccgcgc gcattaatct ctccccttcc ccggtcgtct
    10981 gaccggggaa tactcttcct ctcccaaatg catcaccttt tcccataata ttaaacaaat
    11041 aaactcatta aaaaaaaatg agcgattttt gacagtcgta gaaaatgata
//
LOCUS       DAZKFZ010000150        11052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000150 DAZKFZ010000000
VERSION     DAZKFZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(124..390)
                     /locus_tag="ACHOQW_003441"
     CDS             complement(124..390)
                     /locus_tag="ACHOQW_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP9574465.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(387..1154)
                     /gene="yjiL"
                     /locus_tag="ACHOQW_003442"
     CDS             complement(387..1154)
                     /gene="yjiL"
                     /locus_tag="ACHOQW_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP9574466.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            complement(1164..2315)
                     /locus_tag="ACHOQW_003443"
     CDS             complement(1164..2315)
                     /locus_tag="ACHOQW_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP9574467.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2431..3702)
                     /locus_tag="ACHOQW_003444"
     CDS             complement(2431..3702)
                     /locus_tag="ACHOQW_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP9574468.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3743..4975)
                     /gene="mdtM"
                     /locus_tag="ACHOQW_003445"
     CDS             complement(3743..4975)
                     /gene="mdtM"
                     /locus_tag="ACHOQW_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP9574469.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5442..6224
                     /locus_tag="ACHOQW_003446"
     CDS             5442..6224
                     /locus_tag="ACHOQW_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9574470.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEERFNEFISELTSRMPQHRERIMTIAERIHNDDCRANS"
     gene            6199..6399
                     /locus_tag="ACHOQW_003447"
     CDS             6199..6399
                     /locus_tag="ACHOQW_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574471.1"
                     /translation="MTIAERIHNDGWLLGREEGEQRILRLLLQNGADPEWIQKITGLS
                     AEQMQALRQPLPERERYSWLKS"
     gene            complement(6642..8054)
                     /locus_tag="ACHOQW_003448"
     CDS             complement(6642..8054)
                     /locus_tag="ACHOQW_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9574472.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            8231..8395
                     /locus_tag="ACHOQW_003449"
     CDS             8231..8395
                     /locus_tag="ACHOQW_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP9574473.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            8492..9373
                     /locus_tag="ACHOQW_003450"
     CDS             8492..9373
                     /locus_tag="ACHOQW_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9574474.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            9421..10584
                     /locus_tag="ACHOQW_003451"
     CDS             9421..10584
                     /locus_tag="ACHOQW_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFP9574475.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(10631..10972)
                     /gene="symE"
                     /locus_tag="ACHOQW_003452"
     CDS             complement(10631..10972)
                     /gene="symE"
                     /locus_tag="ACHOQW_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFP9574476.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
BASE COUNT         2871 a         2775 c         2802 g         2604 t
ORIGIN      
        1 gtgctatcaa aaatcgctca ttttgtggat gggaaatatt tcacggcagg cgagatattt
       61 actacaactt ccttcatgca ggattatgcg cagccaatta tccggttggt atatttaaag
      121 atcctattgc ctggttgacg cttctgcatc gtaatgcacc acgcactgcc agtcgctgcc
      181 ctgctggcaa tatatcgctt cggtttgccc agggatcacc cattgtattt cctcgccggg
      241 gggacaggtc agccaaatgc ataacccgca gccgccgcgt aaatcacggg gaatatcgct
      301 gacgcgaaaa gtcatgcccg ctgcctgcaa cgccttgcgg gtttgtatga cgccgaccgt
      361 ggagtgaaat aaaaataaaa actctttcat cggcgtgtcc tcactcgttg accaattacc
      421 gccgcgccaa ttgtgccagc aaattgcgca tcaggatggg tatttaccgg cattcgcagg
      481 tgagattcca gcatccgggc aaacgtctgg caatgactaa cgccaccagt aaacagaatc
      541 ggcgcttcac aggagagacg agcaatgaaa ttggcactcc gccgcgccat cgcgttaatc
      601 actccggcga gaatcgcttc tggcgcgacg cccgctgagc gcaggctgat cacttctgat
      661 tcagcaaaca ctgtgcacat actcgtgatg gcgtgcggcg tgacattttc ggtaatgcag
      721 tcgagttgct cgacgctggt gccaagcgtg cgcgagatca cctccaggaa acgcccggtg
      781 cccgccgcgc atttgtcatt catcaggaaa tcgcacaggt taccgtcatc atcaagctga
      841 atcactttgc tgtcctgacc accgatgtcg attaccgcgc gcgttgctgg cgcaagaaac
      901 cgtgcgccca gcccgtgaca ggagatttcc gtcacttgtt tatcggcaaa atccaccagt
      961 tgccgcccgt agccggtgag cgtcagaaac ggcgttgtct ctaacccttc gcgcagagtt
     1021 tcccaggctt cagtaattgc tgttgccggt cgaaagggtg ttggaacgag gaaacggcgc
     1081 gtaatcacgc cgtctgcgag taagatccct ttggtggcgg tagaaccaga atcaatgcca
     1141 atcgaatatg tcactgctac tccttacagc atctcaataa aggccgcgac acgggtactg
     1201 agctgcccaa catccgaggt ggagtagtct gtttcaatag cgatataagg aatgttgtgc
     1261 tgctgacgca catgacgttt aattgccagc gattccaccg cgtaggtatg gcacgcctgc
     1321 aaaatcacat caactacgcc atcgacctga tattcttcca ccatctggct gagcattttc
     1381 aggcgctgat cgttcggcga aacacaggag cagccaatcg ccagatattt atccgccagc
     1441 gcgtcgtaga catcgcccgt ttctgccacg cattgctcgg tcgctttcgc cccggtgcag
     1501 ttttcataac cgacaaccca gccgccattc tcttcaatcg cgcgcaccac tttctctgct
     1561 gcgccgccaa tcgggcagcc ggtgattaaa atgcgcggac gcgggtccag tcgctggcct
     1621 tcttcccact gctgacgaat gcgggcggtc atcgcgtcca gttcattgat caacgcctct
     1681 ttatcgaacc ggaaggttgc gccgtaaacc actttcagaa tgtcgctgcc gctaagcgcc
     1741 ggaggattga actgcccaag atgataaaaa ttggccagtg cgcgacgttc gcggtttttc
     1801 agcgcaatgg catcgcgcag agcatcttcg ctaatttcgt gcccaaaacg ttcttccacc
     1861 gctttttgta agcgcagcat ctcggctttc cataacgcac gcgaggcatc gtctttaacg
     1921 ctgtttggca gctgcatcac atgaacgggc ttaaactccg ccatgtattc atacattttc
     1981 tttttgccgt cgcaggtggt ttcaccgacc accagatccg aaaagtagaa gtaggggcat
     2041 ttatcggttt tgccgaagcc gtagctactt ttaatcagcg ggcagaggtt gcgcggcaga
     2101 tctttctccg cttcttcaat ggtttcatca gaggtggaac agagcgaaac cacaaccgca
     2161 ccggctgcca tcgggatctc ttgcggcata aaggtgcagt aagtgccaac cagcggaatg
     2221 ccgcgcacct tgagatccat gacggtgaga aagcctttct ggcgagcttc agagaactga
     2281 tcgaaaatgg cgggtagatc ggtgacaagt gacatgattt tccttccccg taccacggga
     2341 gataatgaaa agaggccaca ttattaccat tcttttagta tggtttattt gatctctctc
     2401 gcggttagcc acttagtttt tcatggattt ctaaaaattg ccgaggggga acaaggccgg
     2461 gagctgtgac agcaaatata aaattagccc aatacaaccg ccaaccagcg taccgttgac
     2521 acggataaac tgcagatctt tgccgatatt taactctatt tgccgcgaca tatcccgggc
     2581 atcccagctt tttaccgtat cgctgatatg gcgcgtcagg aatgcagaaa actcaggcgc
     2641 gacgcggtgc gcggcttgtt ccaggtgacc atttaacgac gcccgcaagg catcatcggc
     2701 aattaacgtt tcgccaaacc attgacctgc ccgcgcgata cgttctttca cacgagaatc
     2761 ttcactgttg atatccgctt tcagccattc ccgtaaatcc ccccacaatt cactgagaaa
     2821 ggcttcatcc tctttcagat agctttttac ggcatcagct cgcgctgtca tttcaggatc
     2881 gtttttcaac ttgtcgatca gggcaaaagt ggcgcgatca aatgcatggc ggatctgatg
     2941 cgcacgatcg cggctgatat catcaagcaa agaattcacc gcgtcagaaa ccaactccgc
     3001 gctatgttcg cccagccatt cggtggggag aattttggct ttcagtggat gctcactctc
     3061 cagccagcga acaatctgct gggcgataaa cttgcgcgat ttatcgcgct ggagaagggc
     3121 gatcaactgt gcgatcagcg tatccagcag cacctgatga cgatcgtttt tggtcatact
     3181 ctccagcatc aacgcactgg tgccggaaag atcgacctta tcaatcgccc gatggaccgc
     3241 gcgcttaagc aggcgctgaa tacgtgcatc atcggtcagt tcaagaaaac cactcatgat
     3301 ctgcaacaga tgctgaccaa cgcggcgggc gttttctggc tggctaaacc agttgccaat
     3361 cagcaacgcc ggttcgtgac gtcgaatcaa tgccaccagc gattgggtat cgagaaattt
     3421 tttctgcacg aactgaccga gattttcgcc aatccggtct ttattacgcg ggataatcgc
     3481 cgtatgacga gaaatgatcg gaatcggcac gcggcgaaac agcgccacca ccgcaaacca
     3541 gtccgccagc gcgccgacca tcgccgcttc agcaatcgcc ttcacgccgc tcacccaaaa
     3601 attgggcggc aaaaacagcg taacgacaaa ggtagcagcg gcgataagca gtaaagagag
     3661 cgccaacatt ttggcgcgtc tgagttcaat gagtttattc ataacgttaa gaatagagcc
     3721 tgacggcagg atcgcgcaaa aatcactgct cctctgccag ctcggcagcc tgatgttggc
     3781 gcacgcgatt aagcaatccg gccagggtga aaacgacgat aacgcccgcc acaacggcta
     3841 acagatgaaa cggcaagcga ccgccgttaa accacagcca gcggccaatt tcgaccgaga
     3901 cagacatcac catcaggatc accatattca gcgatgcgga gacggtccct ttcggtaagt
     3961 tattggaaaa cagcgtaaag cggaataagg tcgggaagat caaaccaatc ccgaaagcat
     4021 acagactggt gcccagcacc gaccacagcc agacgtgcgg tgacagcaga ttgccgataa
     4081 tcaacagcgc gaggccgacc agttgaatgg gtacggcacg ccagataaag cgcggttcgg
     4141 tcggatcttt aacgaaacgc gccacgatgg cattcgcgac aatcaccgcg ccgaacaccg
     4201 gaacctgtgt ccaggcgaac tgcgaggtcg ttaagccgcc tgcgtcaatc aggatcaccg
     4261 gcgagacagc cacccaactc atcatgggga tatagcttaa agagatggtt gctgcgccaa
     4321 agaggaacag ccgattgcaa aagacattac gaaaatcgcg caagacgctt ttggcgctaa
     4381 atggaaccgc gccgcgcttc accgtctctg gcatcgccag cagtaagcca acaaatgaga
     4441 taaaacccat aaccgcaatg atggcaaaaa ggactttcca gtgcacaaag tgcatcagag
     4501 ctgcgccgga aagcgggccg ataatcggcg caatcagtac gatggaggtg ataatcgcca
     4561 tcaacttgat cccttttgtc tggccgaacg cctcctgcac cgtgacataa ccaacggtgg
     4621 caataaaaca gatactggtg ccctgaattg cacgcgcgat aagaaactgt gtcatagacg
     4681 ttgtgaacat tgtcgcagcg caggcaaggg taaatattag cgccccggta atcagcactg
     4741 gcttgcggcc aatcctgtcg gaaagcggcc ccagcagcca ctgcaacgcc atgcctccag
     4801 cgagatagag actgacggca gccggagcca gactgacatc ggcattaaaa tcacggacca
     4861 cattaatgat cccaggctgg atcaggtccg tcgaaagata ggcggcaaag tcatacaaaa
     4921 tcaacgccat cgggaaaaac agcgtggcgg catggcgggc aaaaaaacgt ggcataacaa
     4981 acaactcctt gtccggagaa ccccaacggg gaaaacgaaa ttcaataccg tcggtaatga
     5041 cgaaaggtag gcgagaagga caaatgtact gaatttcatg taatcaacca cactgcctgt
     5101 gaacacacag acagagaaat accttctgac ttcgggtcag aatgtataac gattacaaat
     5161 agtcatcagc acattacacc tcttgaattg atgggttgca tagaaaatat acaccttaag
     5221 tgtaattaaa atttgcgatt aatcaaaaga gcgcggtgga agggggaata tctgccgggt
     5281 acaggacatg taaaacgcga gtcagtttcc ggaaaaatgt atcgtttgca gacgaacaaa
     5341 aatagcctgg aaagcgcctc gggaaacgag aaattgccgg gtgagaatgg ttttgttagt
     5401 cgctaaagtc gggccatctt atcaacaggt gacggatcgc catgacaaac ttcacgacca
     5461 gcacgccgca tgacgcatta tttaaatctt ttctcatgca ccctgacacc gcgcgggatt
     5521 ttatggagat ccacttaccc aaagatttac gtgaactgtg cgatctcgac agcttaaaac
     5581 tggaatccgc cagctttgtc gatgaaaaat tgcgggcgct acactccgat attctgtggt
     5641 cggtaaagac ccgggaaggc gatggctata tctatgtggt gattgaacat cagagccgtg
     5701 aggatattca tatggccttt cgcctgatgc gctattccat ggcagtgatg cagcgccata
     5761 tagagcatga taaacgccgg ccactaccgc tggtcatccc gatgctgttt tatcacggta
     5821 gccgtagtcc ttatccctgg tctttgtgct ggttggacga atttgccgac ccgactactg
     5881 cacggaagct ttataacgcc gcgttcccgc tggtggatat cactgtcgtg ccggacgacg
     5941 agattgtgca gcaccgcaga gtcgccctgt tggagttgat ccaaaagcat attcgccagc
     6001 gcgatctgat ggggcttatc gatcaactgg tagtattact ggttacagag tgtgctaatg
     6061 acagccagat aactgcgctg ttaaattaca ttttactgac tggcgatgaa gagcgtttta
     6121 atgagtttat cagtgaacta accagtcgaa tgcctcaaca cagagagcga ataatgacga
     6181 ttgcagagcg aattcataat gacgattgca gagcgaattc ataatgatgg atggctgttg
     6241 ggaagggaag aaggggaaca gcgcattctt cgattgttgt tgcagaatgg ggcagatcct
     6301 gaatggatac agaagattac cggactttcg gcagagcaaa tgcaggcatt aaggcagccc
     6361 ttgcctgagc gtgagcgcta ttcatggctc aagagctaac cagagacgga tgacaaacgc
     6421 aaagcagcct gatgtgctac gcttatcagg tctacaaagc ccattcaata tattgaactt
     6481 taaagatttt gtagacctgg taaggcgttc acatggcatc cggcatgaac aaagcgcact
     6541 ttacctaagc catgccggaa cgtggctatg atcctgcaaa gataaaacga gtcacaggcg
     6601 attcgtgaga ataaatcagg tgcttcgcca ctaattctaa tttattccat tgcccgatac
     6661 acggcctcgc caatttgctt cagcgcttcg cgataagttt cgctgagtgg caaagcacag
     6721 ttgatgcgca ggcagttacg gtatttgccg gaagctgaga aaatcgagcc tgccgccacc
     6781 tggattttca tgcggcacag ctgccgcgtg acgcagacca tatcgacctg ttcaggcaat
     6841 tctatccaca gtaaaaatcc gcctttcggg cgcgtaatac agatttcgca gggaaaatat
     6901 tcccgtatcc agcaggtata aagcgccaaa ttgcgctgat agatctgccg catccgccgg
     6961 atatggcgat gatagtgacc ttccagcaca aacgttgccg ccgccatttg cgtggacggc
     7021 acattaaagc tgctgatggc gtatttcata tgcatcagtt tatcgtgata acgccccggt
     7081 gcgacccaac ccacgcgcag gcctggagca atacttttac tgaacgagct gcacaacagc
     7141 actcgcccgt cgatatccca ggaatgaatg gtccgcgggc gcggatactc cgttgccagt
     7201 tcgccgtaga catcatcttc aaaaatcaca atatcatgac gctgagcgag agagagaacg
     7261 gcccgtttgc gcgcgtccgg cataataaat cccagcggat tattacagtt tggcaccaga
     7321 atgatgcctt taatcggcca ctgttccagc gccagttcca gcgcttcaac gctgatgcct
     7381 gtttctggat cggttgggat ttcaatcact ttcacgccca tgccgcgcag catctgcatt
     7441 gaaccgtaat aacaggggga ttcgaccgcg acaatatcgc ccggtttaca caccgccatt
     7501 aacgctagcg acatcgagtt atggcagccg ctggtgatga tgatgtcatc ggcagtgacc
     7561 accgagccgc tgtcgagcat caggcgggca acctgctcgc gcaatacccg ctgaccggct
     7621 agcaagtcat aaccgagaac ggtttgcaga ttatgctgca ccacccggct tagctcccgc
     7681 cacagcggtt tcaggctggg cgtttcgaca tccggcgtgc ttttgcttaa cggaacaatg
     7741 gaactgtcgc tatgcgccac cagcatatcc agcaccagat cccactgggt aatttccact
     7801 gggcgctgca ccggacgcgt catcggcggc actggcggct gggctttacg ttgtgcgaca
     7861 aaataacccg aacgcggctg cggagtgatg agcttcatcg tctccagcgt ctggtacgcc
     7921 tgctgcacgg tgctgatgct gacgccgtgc tcctgactta agctgcgcac cgacggcaat
     7981 ttctccccgt gacgatacag cccttgctca atccgctcgg caagtagagt cgccagatgt
     8041 tgataacgcg tcatgctgta ttccttattt ggaccataca gaaagaaaaa ccggtacaga
     8101 tgaggctaaa aaaccggatg cagatgctgt tttaacctgt ctgtatgtta aacaaaacta
     8161 atatttgcat ctgtattgtg aactgccaga agacggatga tgaagcctct gaaatatgag
     8221 gaggcagcag atggaatttc acgaaaacaa agccaaaacg ccgtttatca gacttgtgca
     8281 actctggcag gcagtgaggc gttggcggcg gcaaatgcag gcccgacggg tgttacagca
     8341 gatgagtgat gaacggttga gggatattgg gttacgcagg gaggatgtgg agtgaggggt
     8401 atgttcgtcg cggataaaca ggatttctct ataacctcca taaggaagtg gttattattg
     8461 cgaaatcatc gtttcacgga aagacgtcag aatgaaagct acagaagctc gcctactcga
     8521 ttttttaaaa cgttcgcagc agtttgttat tcccatttat caacggactt attcatggac
     8581 agaacaacaa tgtcggcaac tttgggacga catcattcgt gcaggaaagc gtgacgatat
     8641 atcagcgcat tttatcggtt cggttgttta tatagagcag ggattgtatc aggtttctgg
     8701 tatttctccg ttgctggtca ttgatggtca gcagcggctg acgaccgcaa tgttgctgat
     8761 tgaggcttta tcgcgccatc ttggcgaaga cgaagttttt gatggctttt cggcaatgaa
     8821 attgcgtaat tattatttgc tcaatcctta tgagtccggc gagaaaggtt ttaaattact
     8881 gctgaccgag actgataaag acagtttact ggcgttaata aaacaaagac caatgccaga
     8941 aaactattcc catcgaataa tggaaaactt tactttcttt gatgaacaaa ttgccaaact
     9001 cggtgatgac ttgatcccct tatgtcgtgg gttagcaaag ttattaattg tcgatgtggc
     9061 gcttaatcgt ggtcaggata atccgcaact gatttttgaa agtatgaact ccaccggtaa
     9121 ggcgttaagt caggccgatc tggtgcgcaa ttttattctg atgggcctcg aaccagagca
     9181 tcaaacccgg ttgtatgaag atcactggcg tccaatggaa gtcgcttgtg gtcagcaagg
     9241 ttacagcgaa tattttgaca gttttatgcg tcattatctg acggtaaaaa cggggagatc
     9301 cctcggacag atgaagtcta tgaggcattt aaactccatg cccgcagcca gagtgttgct
     9361 gaaaaaggcg tagatcggct ggttgaagat attcatatct acgcggagta ttactgtgca
     9421 atggcattgg gaaaagaaag tgacaaatcg cttgctacgg cttttcagga tttgcgcgag
     9481 ttaaaggttg atgtggcgta tcctttctta ctggcgcttt atcatgacta taaaaatgac
     9541 gatttgtctc acgaagattt cctgagcata attcgtttaa ttgaatctta tgttttccgc
     9601 cgtgcagtat gtgcgattcc gacaaattct cttaacaaga cgtttgctac tttttataag
     9661 gtcattaata aagaaaaata tctggaaagt attcaggtac attttttgaa tctaccttca
     9721 tatcgtcgtt tccccaacga tgatgaattt aaacgggaat taaaagttcg cgatctctat
     9781 aacttccgta gtcgtagcta ctggttacga cgactggaaa acgataaacg cagagagcgc
     9841 gtggaagagt ttacgattga acatattatg ccgcagaacg aaaatttgtc ggctaaatgg
     9901 cgcgaagagc tgggaagtga ctggcagcgt attcataaag aactgttgca tacgttgggg
     9961 aatctcactt taacgcgcta taactcccgt tacagtgaca gacctttcgc agaaaaacgc
    10021 gatattgaag acggctttaa gcatagcccg ctctatttga atatcggtct tggacagtgc
    10081 gaaaaatggg atgaagctgc cattcacgcc cgagccgatc gtctggcgga gcttgcggtt
    10141 caggtctggc aagcgccttc tcttcctgaa gaggttttag ctgtttatcg gggacagcct
    10201 gagaacaaaa ccagttacag cctgagtgat tatccttttc ttgctgatgg ttcgcatagc
    10261 cgggtgttat tcgatcatct tcgcgatgaa attatgcgcc tggacgcagg gatcacgcag
    10321 gaagtattga agctctatat tgcgtttaaa gcggaaacga attttgttga tgttgtgccg
    10381 caaaaaagcc gactgcgatt gtcgcttaat atgcagtttc atgaactggt cgatccgaaa
    10441 ggtattgcca aagatgtgac aaatgttggg cgatggggca atggtgatgt ggaaattggt
    10501 ttcagtgacc tcgcacaact tccttacatt atggggttaa ttcgtcaggc atttgaaaaa
    10561 cagatggaga gcgcgttggt ataacgcagc gaaaaatgcc cggtagtcct ggcgctaccg
    10621 ggtcaatgta tcaggcaact ttctgtttac ccgcaatcac cccaataaac tcctgcacct
    10681 gcctttgttt acgcgccgac agcttgcaca cctggcgcag cgactgcatc agttcgctct
    10741 cggcggcggc gggtggttgg gcggtgagga caatacagcc ttccatcact ttgacatcta
    10801 ccaccgtgcc ggtggcaaaa ccggcggctt ccagccactg acctttcagg gtgatggcgg
    10861 gaatacggct gtaatccggg taacgactcg cataactcac ggttaattga cggttatttg
    10921 ccggggagac ttctgcttcg aacggttgtg caatagaatg cgtatcagtc ataactgcta
    10981 ttccctataa atagtgattg tgattagcgg tgcgggtgtg ttggcgcaca tccgcaccgc
    11041 gctaaatacc tg
//
LOCUS       DAZKFZ010000151        11035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000151 DAZKFZ010000000
VERSION     DAZKFZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..939
                     /locus_tag="ACHOQW_003453"
     CDS             <1..939
                     /locus_tag="ACHOQW_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFP9574477.1"
                     /translation="NPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAV
                     AGIRYQYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSS
                     IASYIGDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAG
                     RVRSRGVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYD
                     IHNMPGNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNV
                     KNLFDKTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(1003..2895)
                     /gene="parE"
                     /locus_tag="ACHOQW_003454"
     CDS             complement(1003..2895)
                     /gene="parE"
                     /locus_tag="ACHOQW_003454"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9574478.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(2924..3505)
                     /gene="yqiA"
                     /locus_tag="ACHOQW_003455"
     CDS             complement(2924..3505)
                     /gene="yqiA"
                     /locus_tag="ACHOQW_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP9574479.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3505..4332)
                     /gene="cpdA"
                     /locus_tag="ACHOQW_003456"
     CDS             complement(3505..4332)
                     /gene="cpdA"
                     /locus_tag="ACHOQW_003456"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP9574480.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4357..4779)
                     /gene="yqiB"
                     /locus_tag="ACHOQW_003457"
     CDS             complement(4357..4779)
                     /gene="yqiB"
                     /locus_tag="ACHOQW_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP9574481.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4780..5409)
                     /gene="nudF"
                     /locus_tag="ACHOQW_003458"
     CDS             complement(4780..5409)
                     /gene="nudF"
                     /locus_tag="ACHOQW_003458"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP9574482.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5614..7095
                     /gene="tolC"
                     /locus_tag="ACHOQW_003459"
     CDS             5614..7095
                     /gene="tolC"
                     /locus_tag="ACHOQW_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP9574483.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7243..7914
                     /gene="ygiB"
                     /locus_tag="ACHOQW_003460"
     CDS             7243..7914
                     /gene="ygiB"
                     /locus_tag="ACHOQW_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP9574484.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            7920..9080
                     /gene="ygiC"
                     /locus_tag="ACHOQW_003461"
     CDS             7920..9080
                     /gene="ygiC"
                     /locus_tag="ACHOQW_003461"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9574485.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9118..9906)
                     /gene="ygiD"
                     /locus_tag="ACHOQW_003462"
     CDS             complement(9118..9906)
                     /gene="ygiD"
                     /locus_tag="ACHOQW_003462"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP9574486.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10049..10822
                     /gene="zupT"
                     /locus_tag="ACHOQW_003463"
     CDS             10049..10822
                     /gene="zupT"
                     /locus_tag="ACHOQW_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP9574487.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(10880..>11035)
                     /gene="yqiD"
                     /locus_tag="ACHOQW_003464"
     CDS             complement(10880..>11035)
                     /gene="yqiD"
                     /locus_tag="ACHOQW_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP9574488.1"
                     /translation="YWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNSTRE
                     ETAEKVE"
BASE COUNT         2717 a         2972 c         2747 g         2599 t
ORIGIN      
        1 aaccccgttt atggcaatac cagcaaatgc acaacggttt cggcgtcgga cagtgatcag
       61 acgatcaaac aggagagcta ctcagcttat gcacaggatg cgctctatct gaccgataac
      121 tggattgccg tcgccgggat ccgctatcag tattacacgc agtatgcggg taaaggccgt
      181 ccttttaatg tcaatactga cagccgcgat gaacaatgga cgcccaaact ggggttagtc
      241 tacaaactga cgccatcggt atccttattt gctaattatt cgcaaacatt tatgccgcaa
      301 tcgtcaattg ccagctacat cggagatctt ccaccggaat catctaatgc ttacgaagtc
      361 ggggcaaaat tcgagctgtt cgatggtata accgcagata ttgcgctgtt tgatatccat
      421 aaacgtaacg tgttgtatac cgaaagtatt ggtgatgaaa ccatcgccaa aacggcaggc
      481 cgcgttcgtt caagaggggt agaagtcgac cttgcgggag cattaactga aaacattaat
      541 atcattgcca gctacggcta taccgatgct aaggttctgg aagatcctga ttatgcaggg
      601 aaaccattgc cgaatgttcc tcgtcatacc ggttcgctat tcctgaccta tgacattcat
      661 aacatgccag gcaataacac actgacgttt ggcggtggtg gacatggcgt aagccgtcgt
      721 tcggcaacca atggggctga ctattatctg ccaggctatt tcgttgccga tgccttcgcc
      781 gcatacaaaa tgaaattgca gtatccggtc acactgcaat taaacgtcaa aaacctgttt
      841 gataaaacgt attacacctc ttccatcgcc acaaataatc tggggaatca gattggcgat
      901 ccgcgtgaag tgcaattcac ggtgaaaatg gaattttgat caaaaaagcc cggtatacac
      961 ttgtataccg ggctatgttt tattcttctg gcgggaggat tatcagacct caatctccgc
     1021 catgtcgcct ttctcttgca accagttgcg gcgatcttcc gagcgtttct tcgccagcag
     1081 catatccatc atcgcgtcag tacgctgatc gtcttcatca tcgatagtca gctgcaccag
     1141 acggcgagtg ttcggatcaa gcgtggtttc gcgcaattgc atcgggttca tttcccccag
     1201 acctttaaaa cgctggacgt tcggcttgcc tttcttgcgt tttaattgct caagtacgcc
     1261 ctctttctct tcttccgtca gcgcgtaata aacctctttc ccgagatcaa tacggtagag
     1321 cggtggcagt gcgacgtaaa cgtgaccgtg tttcaccaac gcgcggaagt gttttacgaa
     1381 caaagcgcag agcagtgtag caatgtgcaa accatcagag tccgcatccg cgaggataca
     1441 gattttgcca taacgaagct ggctcagatc gtcgctgtca ggatcgatac cgatcgctac
     1501 cgaaatatca tgcacttcct gcgaggccag cacttcgtcg gaagaaactt cccaggtgtt
     1561 aaggatctta cctttcagcg gcatgatcgc ctgatattcg cgatcgcgcg cctgcttggc
     1621 agatccgcct gcggagtcac cttccacgag gaacagttcg gtacggttaa ggtcctgcgc
     1681 ggtacaatca gccagtttgc caggcagcgc cgggccgctg gtcagctttt tacgcaccac
     1741 ttttttagcc gcacgcatac gacgctgggc gctggaaatc gccatctctg ccagcaactc
     1801 cgccgcctga acgttctggt tcagccacag gatgaaggca tctttcacca cgcccgaaac
     1861 gaatgccgca cactgacgcg aagagagacg ctctttggtc tgcccggcaa actgtggatc
     1921 ctgcattttt actgacagca cataggcgca gcgatcccag atatcttccg ccgacaactt
     1981 tacaccgcgc ggcaaaatgt tgcggtattc acagaactca cgcatcgcgt ccagcaggcc
     2041 ctgacgcagg ccattaacat gggtaccgcc ctgcatcgtt gggatcaggt tgacgtagct
     2101 ttcggtcagc agttcaccgc cttccggcag ccacagtagc gcccagtcca ccgcttcagt
     2161 atcgccagcg aaattaccga taaacggttt ttctggcagc gtcggtaaac cgtttaccgc
     2221 ttccgccagg taatcattca gaccgtcctg atagcaccag cgctgttcgg tgttgttgat
     2281 ctcatcttta aaggtaatct caacgccggg gcacagtact gctttggctt tcagcacatg
     2341 cgtcaggcgt gaaacagaaa aacgcgggct atcgaagaag gtttcatccg gccagaaatg
     2401 gacgctggtg ccggtattgc gtttaccgca attgccgaca acctgtaaat cctgcacctt
     2461 ttcgccattt tcaaaggcga tgttataaac ctgaccatcg cggcgcacgt taacttctac
     2521 gcgcttcgac agggcgttaa ccaccgaaat ccccacgcca tgcaggccgc cagagaactg
     2581 gtaatttttg ttagagaatt taccgcctgc atgcagacgg caaagaatca gttcaaccgc
     2641 cggtacaccc tcttccgggt gaatatccac cggcatcccg cgcccatcgt caataacttc
     2701 taacgactgg tcagcatgta aaataacgtc cacgcgtttt gcgtgacccg ccagtgcttc
     2761 atccacactg ttatcaatga cttcttgccc caaatggtta gggcgagtgg tatcggtata
     2821 catccccgga cggcggcgaa ccggctcaag cccggtgagt acctcaatgg catcagcgtt
     2881 ataagtttgc gtcatggttt aagttagcaa ttcgagttga tcgtcagaga tggtgcagac
     2941 caagaaaatc gacgatcggg ttgaaataat cttcgaagcc cgtgaatgcg tggttgccgc
     3001 cttctatgac agtctggcgg caggaagcgt agtacgccac cgcctggcgg taatccagca
     3061 cttcatctcc cgtctgttgc agcagccaga tcaaatccgg cgcttccagc gggtcaatct
     3121 gcatgacttt aagatcgtaa atatggcgtg actctagcac atattgctgc ccggtgtagg
     3181 ggttctcgtt ctgaccgaga tagtccgtca gcagttcaaa cgggcgcacc gccgggttta
     3241 ccaccactgc gggcagcata aaacattgtg acaaccaggt ggcgtaatat ccccccagtg
     3301 acgaaccgac aatacccagc gaatcgccgc catgttccag gacaatggat tccagcagct
     3361 ctgccgcgtc ggaaggatac ggcggcaact gcggaatgat catctcaacg tcagggtgat
     3421 gttccgccag ccagtttttt aacaagctcg cttttgcaga gcgcggcgag ctgttgaaac
     3481 cgtgtaaata aagaagcgta gacatcagta gccttctgaa gcggtatcag gttggaaacg
     3541 tgtgtccgcc aggcgatgca cctcggtggt cagcgtgcca tcagcatgta actcgagagt
     3601 acgccagccg ggcgcgatag tatccagcgt aaagttggaa cagtgcggct taaactgcac
     3661 acaggtcgac ggtgttgcca gcaggcggcg accgttccag tcgagatcca gctcctgatg
     3721 aatatgaccg cacagcaagt atttgacgtg cggaaacttc gccagcacgg tatccagttc
     3781 gcccgcgtta cgcaggctgt gttgatcaag ccaactacaa cccgcaggta gcggatgatg
     3841 atgcagcagc agcaacgtat ggcgctctgg cgcatcagcc agtttacgtt ccagccactc
     3901 aagctgaaac tcgcttagct caccgtgcgg cacgccaaac acctggctat ccagcaacag
     3961 gatttgccat tgctcaccaa taaacacccg cttcgccggg gagatacccg catcctgtaa
     4021 cgcgctgtac atcgcgggct ggaaatcgtg gttgcccggc agccagacgc agggcgcacg
     4081 aaaacttgcg atgccttcag cgaaatgctg ataggccgca gaggattgat cctgcgctaa
     4141 atcacctgtc gcgacaatca ggtcgaattc gtgctggtgt ggacgaatcg cctccagcac
     4201 cgcctggtag ctttcccagg tgtttacccc taagagggct tcgtgctttt gtgcaaacag
     4261 gtgagtatcg gtaatttgta aaatcctgac tctggcctca ccagccagag gaagggttaa
     4321 caggctttcc aaatggtgtc cttaggtttc acgacgctaa taaaccggaa tcgccatcgc
     4381 tccatgtgct aaacagtatc gcaaccagtc cgctaaaaac tggttaattt gatgcttttc
     4441 gtcgcgttga tgcaactttt tattaggata atcataccgc gctttgaagc gaaaaatctg
     4501 ctggcttgaa cacacttcag ccaccatcgc gtcatgatac agacgcaccg tcattgacgg
     4561 aaggctccag taactgatcg cgggcgcagt ctgttctatt gtcaccaggg tagtgtatcg
     4621 ggtcgattcc acaatcgtca gccgatattg tgcgtttgcc acctgatagc ttacagtttc
     4681 gccgggtgcg tcattgcgcg gtaacaaacg gcgcaattgt gaaaagttca tctcgcacag
     4741 gcgcatcatt tcaggaaagt caggtgtgta acgcttcatt tatgcccact cattttttaa
     4801 cgcttgatga tgcagctgca gccattgcaa agcgatgacc gacgctgcgt tgtcgatttt
     4861 cccctcttct acccactggt atgcctgttc ccggcttacc acatgaacgc gaatatcttc
     4921 gttttcatca gccagaccgt gaataccgct tgcggtcgtg gcgtccactt cgcccaccat
     4981 aattgacgaa cgctcactgg tgccccccgg gcttgccagg aaacttaaca ccggtttggt
     5041 ccgtttgact atcagccccg cctcttcaat cgcttcgcga cgggcaacat cttccacact
     5101 ttcaccctct tcaatcattc cggcaaccat ctccagtagc caaggggttt cgctagtgtc
     5161 gtatgcggca attcgaatct gctcaatcag cacaacttca tcacgcactg ggtcaaaggg
     5221 tagcaagact gcggcgtgac cgcgctcaaa aatttcccgc cgtacctcat gactcatttg
     5281 cccgttgaat agacgatgac gaaatctata aagatctaat gaaaaaaagc cgcgataaag
     5341 tgtttctcgt gcaataattt ctacatcgtt tttgccaaat gtaacgggca ggttgtctgg
     5401 cttaagcatt gttaatgtcc tggcactaat agtgaattaa atgtgaattt cagcgacgtt
     5461 tgactgccgt ttgagcagtc atgtgttaaa ttgaggcaca ttaacgccct atggcacgta
     5521 acgccaacct tttgcggtag cggcttctgc tagaatccgc aataatttta cagtttgatc
     5581 gcgctaaata ctgcttcacc acaaggaatg caaatgaaga aattgctccc cattcttatc
     5641 ggcctgagcc tttctgggtt cagttcgttg agccaggccg agaacctgat gcaagtttat
     5701 cagcaagcac gccttagtaa cccggaattg cgtaagtctg ccgccgatcg tgatgctgcc
     5761 tttgaaaaaa ttaatgaagc gcgcagtcca ttactgccac agctaggttt aggtgcagat
     5821 tacacctata gcaacggcta ccgcgacgcg aacggcatca actctaacgc gaccagtgcg
     5881 tccctgcagt taactcaatc catttttgat atgtcgaaat ggcgtgcgtt aacgctgcag
     5941 gaaaaagcag cagggattca ggacgtcacg tatcagaccg atcagcaaac cttgatcctc
     6001 aacaccgcga ccgcttattt caacgtgttg aatgctattg acgttctttc ctatacacag
     6061 gcgcaaaaag aagcgatcta ccgtcaatta gatcaaacca cccaacgttt taacgtgggc
     6121 ctggtagcga tcaccgacgt gcagaacgcc cgcgcgcagt acgataccgt gctggcgaac
     6181 gaagtgaccg cacgtaataa ccttgataac gcggtagagc agctgcgcca gatcaccggt
     6241 aactactatc cggaactggc ggcgctgaat gtcgaaaact ttaaaaccga caaaccacag
     6301 ccggttaacg cgctgctgaa agaagccgaa aaacgcaacc tgtcgctgtt acaggcacgc
     6361 ttgagccagg acctggcgcg cgagcaaatt cgccaggcgc aggatggtca cttaccgact
     6421 ctggatttaa cggcttctag cgggatttct gacacctctt atagcggttc gaaaacccgt
     6481 ggtgccgctg gtacccagta tgacgatagc aatatgggcc agaacaaagt tggcctgagc
     6541 ttctcgctgc cgatttatca gggcggaatg gttaactcgc aggtgaaaca ggcacagtac
     6601 aactttgttg gtgccagcga gcaactggaa agcgcgcatc gtagcgtcgt gcagaccgta
     6661 cgttcctctt tcaacaacat taatgcttct atcagtagta ttaacgccta caaacaagcc
     6721 gtagtttccg ctcaaagctc attagacgcg atggaagcgg gctactcggt cggtacgcgt
     6781 accattgttg atgtgttgga tgcgaccacc acgctgtaca acgccaagca agagctggcg
     6841 aatgcgcgtt ataactacct gattaatcag ctgaatatta agtcagccct gggtacgttg
     6901 aacgagcagg atctgctggc actgaacaat gcgctgagca aaccggtttc cactaatccg
     6961 gaaaacgttg ccccgcaaac gccggaacag aatgctattg ctgatggtta tgcgcctgat
     7021 agcccggcac ccgtcgttca gcaaacatcc gcacgcacta ccaccagtaa cggtcataac
     7081 cctttccgta actgatgacg acgacggggc ttcggccccg tctgaacgta aggcaacgta
     7141 aagatacggg ttatctgccg cattcttccc ccttctcgct tcaatttcga ccagccatcc
     7201 tctattctga tgggtattta ccacaggtcc cggaagacaa aaatgaaacg gacaaaatcc
     7261 atacgccacg catcgttccg caaaaactgg agcgcacgcc atctgacacc agtcgctctc
     7321 gcggttgcca ctgtttttat gctggctggc tgtgaaaaga gtgatgaaac agtgtctctc
     7381 tatcaaaatg ctgacgactg ttcagctgca aacccaggca aaagcgccga atgtaccacc
     7441 gcgtacaaca atgcgctgaa agaagccgaa cgtactgcgc cgaaatacgc cacccgtgaa
     7501 gactgtgttg ctgaatttgg tgaaggtcag tgccagcagg caccagccca ggctggcatg
     7561 gcaccagaaa accaggcgca ggcccagcaa tccagcggga gtttctggat gccgctgatg
     7621 gccggttaca tgatgggtcg tctgatgggc ggcggtgcgg gatttgcaca gcagccgctg
     7681 ttctcctcga aaaacccagc cagcccggct tacggtaaat ataccgacgc gacgggtaaa
     7741 aactatggcg cagcccagcc aggccgcacc atgaccgtac cgaagacagc catggcacca
     7801 aaaccggcga ccaccactac cgttacccgt ggcggttttg gtgaatctgt tgccaaacaa
     7861 agcactatgc agcgtagcgc aaccggtacc tcttctcgtt caatgggtgg ctgataccga
     7921 tggaaagagt cagtattacc gagcgcccgg actggcgtga gaaagcccac gaatacggtt
     7981 tcaattttca caccatgtac ggcgagccgt actggtgtga agatgcttac tacaagttga
     8041 ccctcgccca ggttgaaaag ctggaagaag tcaccgccga actgcaccag atgtgcctga
     8101 aagtggtgga aaaagtgatc gccagcgatg agctgatgac caaattccgc attccaaaac
     8161 acacctggag ttttgtgcgc cagtcatggc tgacgcacca gccatcgctt tattcgcgtc
     8221 ttgatctggc gtgggatggc actggcgaac ctaaacttct ggaaaataac gccgatacgc
     8281 caacgtcact atacgaggcg gcgttctttc agtggatctg gctggaagat cagcttaacg
     8341 ccggtaactt gccggagggc agcgaccagt ttaacagtct gcaagaaaaa ctgatcgatc
     8401 gcttcgttga gctgcgtgaa cagtatggct tccagttgct gcatctcacc tgctgtcgcg
     8461 acacggtgga agatcgcggt accattcagt atttgcagga ctgcgcgacg gaagctgaaa
     8521 tcgctaccga gttcctctac attgacgata tcgggttagg tgaaaaaggt cagttcacgg
     8581 atttacagga tcaggtaatt tccaacctgt tcaagctgta tccgtgggaa tttatgttgc
     8641 gtgagatgtt ttccaccaag ctggaggatg caggcgtacg ctggctggaa ccggcgtgga
     8701 agagcattat ctccaacaag gcacttctac cgctactgtg ggagatgttc ccgaatcacc
     8761 cgaacctgct gcccgcttat tttgcggaag atgatcatcc gcaaatggaa aaatatgtgg
     8821 ttaaaccgat cttctcccgt gaaggcgcaa acgtgtcgat cattgagaac ggcaaaacca
     8881 ttgaagcagc ggaaggtccg tatggcgaag aagggatgat tgttcagcaa ttccacccgt
     8941 taccgaaatt cggcgacagc tatatgctga ttggtagctg gctggtgaac gatcaacccg
     9001 ccggaattgg cattcgtgaa gaccgtgcat tgatcaccca ggatatgtct cggttttatc
     9061 cacatatttt tgttgaataa tcaatgatgc cggatggcat tcgccatccg gtaattgtta
     9121 acctatctgc accgacagca tacttaaact gcccatttct ataccatcga ccggaatggt
     9181 aattggctcc tgcccatccc acgcacctaa gacatacagc aacggcagat agtgctctgg
     9241 cgttgggttc gataacgcgc caccttcatg gtcgagataa ttcaccagag gatgctgctc
     9301 taccggccct tgccacgtca gattcgcttt cacatactca ttaaacgaca tcgcccaagg
     9361 atacggtgaa ctatcaccgt gccacttcac tgtacgcagg ttatgcacca cgttgccgct
     9421 ggcgaccaac attattcctt catcacgcag cgccgcgagt tttcgcccca tttcgaaatg
     9481 ccaggcggca ggtttgctac tgtcgatact caactgcacc atcgggatat ccgcgtcagg
     9541 atacatctta atcagcacgc cccaggaacc gtggtcaaag ccccaggctt ctttatccag
     9601 cgtcaccggg atcggcgcta acagctcaac cagacgctgt gccagcgcag gcgaacccgg
     9661 agcaggataa tgcgtatcgt acagcgcctg cgggaagcca ccaaagtcat gaatcgtggg
     9721 cggcgtctcc atcgcggtca ctcctgttcc acgggtaaac cagtgagccg aaactaccac
     9781 aatcgcttgc gggcgtggca atgtcatccc caacgtctgc cagctgcggg tatacaaatt
     9841 atcttccagc acgttcatcg gactaccgtg acctaaaaac aatgctggca tacgtgttga
     9901 agacatgatg atatccttaa ctaaaggtgt cattttgata tcctcacgat acgcttgttc
     9961 ggcggagtaa gaacccggat aacaatgatg atgatcatca gttattttga cgatctgcct
    10021 gaaggtgaag atttataagg agttgtcgat gtcagtacct ctcattctga ccatactggc
    10081 gggggcagcc acgtttattg gcgcgtttct cggcgttctc gggcaaaaac cctcgaaccg
    10141 cttactggcg ttttcgctag gatttgcggc ggggatcatg ttgctcatct cattaatgga
    10201 aatgcttcct gccgcactag cagctgaagg aatgtcgcct gtgttgggtt atggaatgtt
    10261 tatcttcggt ctgcttggct attttggcct ggaccgcatg ttgccgcatg cgcatccgca
    10321 ggatttaatg caaaaatcgg tgcagccgtt gccaaaatcg atcaagcgca cagccattct
    10381 gctcactctc ggcatcagtc tgcataactt cccggaaggg attgccacct ttgtcacggc
    10441 gagcagcaac ctggagctgg gatttggcat cgcactggcc gtcgcgttgc acaatattcc
    10501 tgaaggtctg gcggttgcag gcccggttta tgcagcaacg ggttctaaac gcacggcaat
    10561 tctgtgggcg gggatttctg gactggcaga aattcttggt ggtgtgctgg cgtggttaat
    10621 cctcggtagc atgatttccc cggtggtcat ggcagcaatc atggcggcgg tcgcaggaat
    10681 tatggtggcg ctctcggttg atgaattaat gccgctcgcc aaagagattg accctaataa
    10741 taaccccagc tatggcgtac tatgtggaat gtcagtgatg ggattcagtt tggtgctgct
    10801 acaaacggcg ggaattggtt aaaagcaaca tcagaagttg tccagcgcag ggacaacttc
    10861 tgctagtttc actaaagcct tattctacct tttcggctgt ctcttctctc gtactgttta
    10921 agtatttgtt ccgcgcttca agcagtgcgt ctctgtcctg gcgaaatgcc cgacaataac
    10981 gtttcaaatg caggaaataa cccaccacaa ccagaacaat caatacaatc cagta
//
LOCUS       DAZKFZ010000152        10923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000152 DAZKFZ010000000
VERSION     DAZKFZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            161..811
                     /gene="csgD"
                     /locus_tag="ACHOQW_003465"
     CDS             161..811
                     /gene="csgD"
                     /locus_tag="ACHOQW_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP9574489.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            816..1205
                     /gene="csgE"
                     /locus_tag="ACHOQW_003466"
     CDS             816..1205
                     /gene="csgE"
                     /locus_tag="ACHOQW_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP9574490.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1230..1646
                     /gene="csgF"
                     /locus_tag="ACHOQW_003467"
     CDS             1230..1646
                     /gene="csgF"
                     /locus_tag="ACHOQW_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP9574491.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1673..2506
                     /gene="csgG"
                     /locus_tag="ACHOQW_003468"
     CDS             1673..2506
                     /gene="csgG"
                     /locus_tag="ACHOQW_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP9574492.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2570..3061)
                     /gene="ycdZ"
                     /locus_tag="ACHOQW_003469"
     CDS             complement(2570..3061)
                     /gene="ycdZ"
                     /locus_tag="ACHOQW_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9574493.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3163..3717)
                     /gene="ycdY"
                     /locus_tag="ACHOQW_003470"
     CDS             complement(3163..3717)
                     /gene="ycdY"
                     /locus_tag="ACHOQW_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP9574494.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3741..4478)
                     /gene="ycdX"
                     /locus_tag="ACHOQW_003471"
     CDS             complement(3741..4478)
                     /gene="ycdX"
                     /locus_tag="ACHOQW_003471"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP9574495.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4533..5471)
                     /gene="ghrA"
                     /locus_tag="ACHOQW_003472"
     CDS             complement(4533..5471)
                     /gene="ghrA"
                     /locus_tag="ACHOQW_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP9574496.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5705..5792
                     /locus_tag="ACHOQW_003473"
     tRNA            5705..5792
                     /locus_tag="ACHOQW_003473"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5739..5741,aa:Ser,seq:gga)
     gene            complement(5942..6784)
                     /locus_tag="ACHOQW_003474"
     CDS             complement(5942..6784)
                     /locus_tag="ACHOQW_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9574497.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6869..7066)
                     /locus_tag="ACHOQW_003475"
     CDS             complement(6869..7066)
                     /locus_tag="ACHOQW_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9574498.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7078..7569)
                     /locus_tag="ACHOQW_003476"
     CDS             complement(7078..7569)
                     /locus_tag="ACHOQW_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9574499.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVGDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7566..7940)
                     /locus_tag="ACHOQW_003477"
     CDS             complement(7566..7940)
                     /locus_tag="ACHOQW_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9574500.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7987..8361)
                     /locus_tag="ACHOQW_003478"
     CDS             complement(7987..8361)
                     /locus_tag="ACHOQW_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9574501.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8524..8745)
                     /locus_tag="ACHOQW_003479"
     CDS             complement(8524..8745)
                     /locus_tag="ACHOQW_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9574502.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8808..9284)
                     /locus_tag="ACHOQW_003480"
     CDS             complement(8808..9284)
                     /locus_tag="ACHOQW_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9574503.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9300..9785)
                     /locus_tag="ACHOQW_003481"
     CDS             complement(9300..9785)
                     /locus_tag="ACHOQW_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9574504.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9876..10694)
                     /locus_tag="ACHOQW_003482"
     CDS             complement(9876..10694)
                     /locus_tag="ACHOQW_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9574505.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         2752 a         2729 c         2726 g         2716 t
ORIGIN      
        1 ctagattgaa atcagatgta atccattagt tttatatttt acccatttag ggctgattta
       61 ttactacaca cagcagtgca acatctgtca gtacttctgg tgcttctatt ttagaggcag
      121 ctgtcaggtg tgcgatcaat aaaaaaagcg gggtttcatc atgtttaatg aagtccatag
      181 tattcatggt catacattat tgttgatcac taaaccttct ttgcaggcga cagctctctt
      241 gcagcacctt aaacaatcgc tggcaattac aggaaaatta cataatattc aacgttctct
      301 ggacgatatc tcttcaggct ctattattct tctggatatg atggaagcgg ataaaaagct
      361 tatccattat tggcaggata ctttgagcag gaaaaacaac aatatcaaaa tattgttgct
      421 aaatacgcct gaagattacc cgtaccgcga cattgaaaac tggcctcata tcaacggcgt
      481 tttttatgcc atggaggatc aagaacgcgt tgtcaatgga ttgcaaggcg tcctgcgcgg
      541 cgaatgctac tttacgcaaa agcttgccag ttacctgatt acacattctg gtaactatcg
      601 ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc tgaataagct
      661 gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg aaaatacggt
      721 taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga cacaagcggt
      781 ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta cgctggattg
      841 tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta gaagtcccgg
      901 gattgttaac tgaccatact gtttcatcta ttggccatga tttttaccga gcctttagtg
      961 ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc agtgcacgat
     1021 ggggaagctg gatcacaata acggtcaatc aggacgttat tttccagact tttttattcc
     1081 cgttgaaaag agacttcgaa aaaactgtcg tctttgcact gattcaaact gaagaagcac
     1141 taaatcgtcg ccagataaat caggcgttat taagtacggg cgatttggcg catgatgaat
     1201 tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta gttctactca
     1261 tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt aatccaaact
     1321 ttggtggtaa cccaaataat ggcgcttttt tattaaatag cgctcaggcc caaaactctt
     1381 ataaagatcc gagctataac gatgactttg gtattgaaac accctcagcg ttagataact
     1441 ttactcaggc catccagtca caaattttag gtgggctact gtcgaatatt aataccggta
     1501 aaccgggccg catggtgacc aacgattata ttgttgatat tgctaaccgc gatggtcaat
     1561 tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag gtttcgggtt
     1621 tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa tcatgcagcg
     1681 cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc cgcctaaaga
     1741 agccgccaga ccgacattaa tgcctcgtgc tcagagctac aaagatttga cccatctgcc
     1801 agcgccgacg ggtaagatct ttgtttcggt atacaacatt caggacgaaa ccgggcaatt
     1861 taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca cggcgatgct
     1921 ggtcacggca ttgaaagatt ctcgctggtt tataccgctg gagcgccagg gcttacaaaa
     1981 cctgcttaat gagcgcaaga ttattcgtgc ggcacaagaa aacggcacgg ttgccattaa
     2041 taaccgaatc ccgctgcaat ctttaactgc ggcaaatatc atggttgaag gttcgattat
     2101 cggttatgaa agcaatgtca aatctggcgg ggttggggca agatattttg ggatcggtgc
     2161 cgacacgcaa taccagctcg atcagattgc cgtgaacctg cgcgtcgtca atgtgagtac
     2221 cggtgagatc ctctcctcgg tgaacaccag taagacgata ctttcctatg aagttcaggc
     2281 cggggttttc cgctttattg actaccagcg cttgctggaa ggggaagtgg gttacacctc
     2341 gaacgaaccc gttatgctgt gcctgatgtc ggctatcgaa acaggggtca ttttcctgat
     2401 taatgatggt attgaccgtg gtctgtggga tttgcagaac aaagcagaac ggcagaatga
     2461 cattctggtg aaataccgcc atatgtcggt tccaccggaa tcctgacaga taaaaatgcc
     2521 ggaattaata atccggcatt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2581 ttttgatttc ctgctcacgg agagccgtct ttgcactacg cgccgccagc cacaggccgc
     2641 tgtttttcat tgcgtaacca aatatcagcc caagcgccag cgaaggtaac accagtttcc
     2701 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2761 gcagcagctg tttggcctga atacacatca gaaacgcgac aataccggtt atgacataac
     2821 tgagaatttc cagatgtggc gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2881 cgccactaag cagagttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2941 caaagtaggc cgtacagccc aggaagcccg cccagcttag taagcccagg gaaacagcca
     3001 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     3061 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     3121 cagcaaatta tctgttgcag ttatgagtga cgcatatcac atttactctt cagaatcttc
     3181 ttccagctcg tcccacattg cactaatggc atcgcgggtt agcggtgcca tggtgcgcca
     3241 gaaaggcgtg gttgcatggg cctccacttt gccaaggaac gcaccacacc agggtaacag
     3301 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3361 ccaggaagcc gcaagcagca atgtgccgat gtgatcggca ggcgtatccg ctaatggcat
     3421 ccctcgttca gaaagaaaag cgcgcacttc cgcttccgtt gcgtcctcaa cccatgcgct
     3481 acgatatggc ggcacggcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3541 ttgcgtcata tcacagcttt tctgcaaacg tgtcagcaac tcatcctgct ccagtggcca
     3601 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3661 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3721 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3781 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3841 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3901 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3961 cagttgtctt cactgccctt acgtgagtgt aaaaatgagg aattattgat ttccagcgcc
     4021 acctgatgtt tcgcggctgc ttcagcaacg gctttaacat ctatctcata tttcggattt
     4081 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     4141 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     4201 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     4261 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4321 aagtgccagt gatgcggcgc atcttccatg tccgggccat gatcggtaat cgcaaaaagt
     4381 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4441 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4501 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4561 ccgcagaccc tctccccttt ttcgagctgg gcaatagtgc gagaaatgta ctccacagct
     4621 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4681 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4741 ttgccgctat ccagcgccgc gagcaggtca tcttccacga catgaacacc acgcgccagg
     4801 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4861 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4921 agttcttccc atccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4981 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     5041 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     5101 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     5161 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     5221 gaaggcttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     5281 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5341 gctaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5401 ggaatagctt tgcgcagtgc ctctatccac cattgggtat cgaacgttgg gtgataaaag
     5461 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5521 gatctacaaa gaccaccagc ataacaaatc cgtggtcggt ggccaaaaaa gcagattttg
     5581 cttattaaaa ctgcatattg attgaagttt gaataaacgc gcgatttttt aaaaaagttt
     5641 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5701 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5761 tatcgggggt tcgaatcccc ccctcaccgc cagattataa gcgcatgtta ttgtttaaca
     5821 tgcgcttttt tattttccct tccagacagt atgccatcgg gtatgccatc tgaaaccggt
     5881 ttatatgccc cgcgatcata tcttatcgga caatttccga tctttgagca tactggcaaa
     5941 gctatactgc cagcatatca ggatagtgtt ttgcgataat atcattcagt ctgtcaacca
     6001 gttctggttt tctgaacgtg atatgcgcgg agcccttctt aaagtatctg atgctgaaca
     6061 tctcatcttc atagctctct ttaccctgaa cagactgaat gtgatcatcc agacgaaggg
     6121 tgatgtttcc ccggttgtcg gggattggtt taccgctgaa caggtgcaac attctttcca
     6181 gatcggcgag tcggtcagcc tgttgtccgg tgataagatg aaatccccac cggtcccatc
     6241 tcaccagatt gttgacgata attttactgc caaatttaca ggggcaattg gttttgtaat
     6301 tccagttcag ccctctgaag acgttgatca cccctcgctc aaacacctca tccttattct
     6361 ggtgcagttg ttcaaatgtg ctcagaatgt tcgcttcgct gatttccgga aaattatcgt
     6421 actccagtga ccggtaccat gtatcacggg cctgcgtatc cattaatgac agcatcccgg
     6481 aacgttgcat caggtcacgc cagatttcgc gatcaagatt ccgggtaatg gctttcattg
     6541 ctgtttcggg tttctccatc agccagcagc cgcagcgaaa atcctgtttc atggcccagt
     6601 ccggggcagt tttacctccg atgctttccg tcagcaccga gatttcagcc agttgtcgga
     6661 taagtgtgtc gatttgcaac agtgtactct ttcgtacagc cagaatgtgg cgaatgctgg
     6721 ttgagcagat aacgtccgta tgcccgttga ccacttccgg acgggagatt gtgatatctg
     6781 acataatcga tattcctttt tgcagataaa tgaaaacgct agccctgtgc aggctggcgt
     6841 cagaggaatg aagtctggtg agtgagcttc accggttctg gtgcagaagc tgcagatgac
     6901 gcagggtacg gacatcctcc cgggcctgtt cgatacaggg caacagtgct gctgaatccg
     6961 ttttatcctc atcgttgtca aagataattc ccgatccgca gtcgatattg tcctgcagcc
     7021 acgcaatcag aatatccagc gctgtttccg tggttaatga tttcatgttg ttcctggtta
     7081 ccagtcgaaa gtgggtaaac ccggcaggca ttcagcgctg gcatccagat gaatgagact
     7141 gacgccataa cgccggatga gtgtgacgac cagacgacgg aacgtttttg acagacccag
     7201 gcgtttaagc cgcagaaccg ggtacgacca cgcatcggta cgcaacagat agccgttacc
     7261 ggtaaaatga atccattctg attcaccgaa gtcactggtc tggtgtgaca gcgagtacag
     7321 ccaggcgttg tccgtttccg tgatatgtgc ggtactgcag cgtatgccgg taagggtagc
     7381 aaatggtggc gtgcattctg ctgaggtctg gtcagattca tcaacaacac gaagggagta
     7441 gccgttatcg ccgaccatgt taatcagttc agcgagctca ataccatcga cgtcaacgac
     7501 aatgcgcccc atgttcagtg cctgtacgtt aacgctgtcg gcttccagtg tcagggccag
     7561 tttcatcatt tcgcctccgg atacttaccc agggtaatgt tatttaccgt tctgtaattg
     7621 tcgcgggtca tcaggccggt cgcccggcga gcccggagga tatcaatact gtttattaac
     7681 tgagagcggg gacaggcgct gaatcccggc tggtcggtac gcaccagcgc gtatttttcc
     7741 acgagaaaat taactgcatc acacagtgaa atgcctgcct caatatgctg ctcaatcaca
     7801 cgttcatcgg caaacggtgt gtcattcagt gtgaggccat aatgctggtc cagcagtcgg
     7861 atgagcagtg tctgccagat ggtgacggga gacgggcagc gagatacctc ccgtacatgt
     7921 gtgtcggata atgttttcat cttaagttct ctgtaactgg tagtgctaaa ggtgagagct
     7981 tcggggttat gaggtggttg cgggtgctgg tgtcggataa acagccagat aaacgtaacc
     8041 acaggagccg agggtgtcgg cttcgcaggt cagccctttt gcattcagcg tgacggtatg
     8101 ctgatatcgg ggattcagtt caccgctggt gagcatgagt tccagttgtt tcatcagcag
     8161 cggaaaggcc tggtccagat ggtatgcatc tgcgtcgctg aagcggcctc tgataccggc
     8221 gcggtcggca aggtaatgca ggcggttacc ctcttgcacc agacgagcgc caaaacaggg
     8281 cctcactgtg cagggaagcc cccaccaggg gcggtcgtga ttgtcatcgg gatgagttat
     8341 cccggagagt ttgtctgaca cgataaaatc ctctctgaaa agtggttaag aatggtttaa
     8401 cgatgacagg tcagccggcg cgcagaccgg tttcggtgaa gatgtatgca ttgatgagaa
     8461 tggcctcatc cacagcggcg tctgaggtca tctggtcgta ctcgtttccc gggtgacgga
     8521 acatcagttc agggtgacac tcatcagccg gacatacggg ccaaaactgt ccttacggcg
     8581 ttctgcaaac acggcaagaa cgccgggaat atcctgcact tcacgaccag tatacgcttc
     8641 agcactgccg tgccagcgat acttaccggt acagaacgga aaaagacggg atgcaggatg
     8701 ctgttggtga atacgcatgg cttcaccacg ggtgatgatt ctcatgatgt gatacctctg
     8761 aagacagaag ataaaagtga aaacaggtgt gatgtggttg tgacgggtta aagcagacca
     8821 tgttcggcaa aggagaaaac ctggttgcca ccgactatca gatggtccgg cacccggata
     8881 tccaccaggc ccagtgcctg taccagacgt tcggtgataa gccggtctgc cttactgggt
     8941 gtgacttcac cggacgggtg attgtgcgcc agcaccacgg cagcggcatt gtggtacagg
     9001 gcgcgtttaa tcacttcccg gggatgaact tccgtacggt tgatggtgcc ggtgaagagg
     9061 gtttcaccgg caatcagctg attctggttg ttcagataca gcacccggaa ctcttcccgc
     9121 tccagtcccg ccatgttcag aatcagccat tcccgtgccg cacgggtgga ggtgaaggcc
     9181 acgccgggtt catgaagatg gcggtccagg gtttgcaggg cccgcagaat gagactgcgc
     9241 tcgccgggcg tcatctctcc gggcagaaag gaaagctgct gcattgtccc tgcctccatt
     9301 cagtcaatga tgcgcataat ggcgctgcat tccggatgct gcagggcgta atcccgcagc
     9361 cggtaatagt ggaccgtcat ggcataacac tccgtacgac aggcatgatg actgtacgtc
     9421 atcagacagg cagcaatacc ggcggcttcc gggctcattt cagcgcggtt gccgttcatg
     9481 gcattgaaca gtacccatgt ttcgtcatca tcgtcatccg gttcgggtgc cataaatgcc
     9541 ccgccgttgt tcagggtgta cagattccag ataccaccgc agtagtcttc gcaaagacgg
     9601 tccatccagc cgaagacacg gggctccagg gtcacccact gtggaatgag gccaaaatgc
     9661 tgcggccaga agctgatgcg ctgttcatca gggacgggag tggcaaccag ctgaggtggg
     9721 ttattccctg atgcagtggt tacggaaaca gaaggtgtgg tggaattatg cgaaacggtt
     9781 gtcatgaggt tattccttat aaaaagtaaa tgaatggaag aagccccggg gcaggaacag
     9841 atgtgagtca ggactgcact ttcagtgaaa cggcatcagc gcatgctctc cagcagcgtt
     9901 tctgccatca cccacaatgc gcgattgagc ttaatgtcgg tatcaatgct gtgaatggcc
     9961 cgggtatgga tacgttttcc ttttgcactg cgaccggaaa tcccgccttt cagcatattc
    10021 tcctggatgg tctgataagc actccacagg tccttaccgt aatcctcccg gcgtcgtggt
    10081 gtcagaatgt cggcggtggt gacgggctga tgttcgtcac cataacggta agtcagtgcc
    10141 gcctgtgcca gcgcctggcg tgccggtggc ggcaggacca gcgactgcat ggcatcacgc
    10201 ttctcctcta tccggtcaaa aacgcccacc acctcgtaag ccccttcaat gactctgtcc
    10261 accacatttc cccggtgtgg aacacgcaat tctcccagag actgaccgca gaccagtcca
    10321 ttggtacaaa tggcacgaaa atatccgggt aacatctggt agctggatga accgtcatgg
    10381 gagttgagca gaataatttc agggacatgc tgaccggtta tctgtccggc ccgccgcaga
    10441 cgcagcatgt gttttgtgta ttcccggcgg ctctggtcgc gcacgcgggt ctggcaggca
    10501 aagaacggct gaaagccttc ctgctgcaga ttttccagga cggtgatagt gggaatgtac
    10561 gcatagtgtt cactgcgaga ggtatgtcgg ttttccccaa aaatactcgg tacatggcgc
    10621 atcagttctt cgtgtgtcag cggacgatca cgacgtatct ggttcgcata accaaaacga
    10681 ctggctaatc tcatgatttg ctccttatga atgattaagc gatataaatg aaaaagccgc
    10741 gtctcccgga gaagacgcgg cctgatgggt gaaatgaatg aggtttattg tctgagaagc
    10801 ccttcactgg cgaaccacgt attaagctgt attccggcat cgccagtgca actgaccttc
    10861 agcattgcgg ataacccgcc ggaaatatgt tcactggttg tttttgtatc ttcccccaaa
    10921 att
//
LOCUS       DAZKFZ010000153        10896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000153 DAZKFZ010000000
VERSION     DAZKFZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            62..823
                     /gene="adiY"
                     /locus_tag="ACHOQW_003483"
     CDS             62..823
                     /gene="adiY"
                     /locus_tag="ACHOQW_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP9574506.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            960..2297
                     /gene="adiC"
                     /locus_tag="ACHOQW_003484"
     CDS             960..2297
                     /gene="adiC"
                     /locus_tag="ACHOQW_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP9574507.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2401..4044
                     /gene="eptA"
                     /locus_tag="ACHOQW_003485"
     CDS             2401..4044
                     /gene="eptA"
                     /locus_tag="ACHOQW_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP9574508.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4041..4709
                     /gene="basR"
                     /locus_tag="ACHOQW_003486"
     CDS             4041..4709
                     /gene="basR"
                     /locus_tag="ACHOQW_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP9574509.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4710..5810
                     /gene="pmrB"
                     /locus_tag="ACHOQW_003487"
     CDS             4710..5810
                     /gene="pmrB"
                     /locus_tag="ACHOQW_003487"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP9574510.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5786..5875)
                     /gene="pmrR"
                     /locus_tag="ACHOQW_003488"
     CDS             complement(5786..5875)
                     /gene="pmrR"
                     /locus_tag="ACHOQW_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP9574511.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5987..7489)
                     /gene="proP"
                     /locus_tag="ACHOQW_003489"
     CDS             complement(5987..7489)
                     /gene="proP"
                     /locus_tag="ACHOQW_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP9574512.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7753..8631)
                     /gene="rdcB"
                     /locus_tag="ACHOQW_003490"
     CDS             complement(7753..8631)
                     /gene="rdcB"
                     /locus_tag="ACHOQW_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP9574513.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMREEIFSVNLV"
     gene            complement(8628..10856)
                     /gene="crfC"
                     /locus_tag="ACHOQW_003491"
     CDS             complement(8628..10856)
                     /gene="crfC"
                     /locus_tag="ACHOQW_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP9574514.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2720 a         2859 c         2688 g         2629 t
ORIGIN      
        1 ttcccggtat cgctttgctt tagcgagaac tggtctttta ttcgcactca ggagtacatg
       61 tatgaggatt tgcagcgacc aaccttgtat tgttttattg actgaaaaag atgtctggat
      121 aagggttaat gggaaagaac ctattagcct taaagctaac catatggcgt tattaaattg
      181 tgaaaataat attatcgacg tctcctctct taacaacact ttggttgctc atattagtca
      241 cgacatcatc aaagattacc tccggtttct gaataaagat ctctcgcaaa taccagtatg
      301 gcaacgtagc gctacgccca ttctcaccct gccatgcctg acaccagacg tctttcgcgt
      361 tgccgcgcaa cacagcatga tgcccgcaga aactgagtca gaaaaggaac gaacacgcgc
      421 attattattc acggtgctat cccgttttct cgacagtaaa aaattccttt cattaatgat
      481 gtatatgtta cgtaattgtg taagtgacag cgtttatcaa attattgaaa gcgatattca
      541 caaagactgg aatcttagta tggtagccag ttgtttatgt cttagcccaa gtctgttaaa
      601 gaaaaagctg aaaagcgaaa acaccagtta tagccaaata atcaccacct gccgcatgcg
      661 ttatgccgta aatgaattaa tgatggacgg taaaaatatc tctcaggtat cacagtcctg
      721 cggctacaac agtacgtcgt actttatttc tgtctttaaa gacttctacg gtatgacacc
      781 gctgcattat gttagtcagc acagagaacg cactgtcgcc tgatttttaa ccttaacgaa
      841 gagctatatt aataacggca tcagcgataa cccggtcgat aataattcaa ctatcgaatg
      901 caggcgtatg atatgacgta atttattgtc acgaagctcg ccttcgcagg agtttaatta
      961 tgtcttcgga tgctgatgct cacaaagtgg gcttaatccc cgtcaccctg atggtgtcgg
     1021 ggaatattat ggggtcaggt gtttttctgt tacctgcaaa cctggcctcc accggcggga
     1081 ttgccattta tggatggttg gtgacgatta tcggtgcgct ggggctctcg atggtatacg
     1141 ccaaaatgtc gttcctcgac ccaagtcctg gtggttctta cgcttacgcc cgccgctgct
     1201 ttggcccgtt tctcggttat caaaccaacg tcctctactg gctggcctgc tggatcggca
     1261 atatcgccat ggtggtcatt ggcgtaggat atttaagtta cttcttcccg attctgaaag
     1321 atccattggt attaaccatc acctgcgtcg tggtgctgtg gatcttcgtc ctgctgaaca
     1381 ttgtcggtcc gaaaatgatc acccgtgtgc aggccgttgc caccgtgctg gcgctgatcc
     1441 ccatcgtcgg gattgccgta tttggctggt tctggtttcg tggtgaaacc tatatggcgg
     1501 catggaacgt cagcggcctg ggcaccttcg gtgcaattca aagtaccctt aacgttacgc
     1561 tgtggtcgtt catcggtgtg gaaagtgcct ccgttgccgc aggtgtggtg aaaaacccga
     1621 aacgcaacgt ccctatagcc accattggtg gggtattgat tgccgccgtt tgctatgtgc
     1681 tctccaccac cgcgattatg gggatgatcc ctaatgccgc actgcgcgtt tctgcctcgc
     1741 cattcggtga tgccgcacgg atggcgttgg gtgacaccgc cggggccatc gtttccttct
     1801 gcgcagctgc gggttgctta ggttcactgg gcggctggac attgctggcg ggtcaaacgg
     1861 cgaaagcggc tgccgatgac ggactgttcc caccgatttt tgcccgtgta aataaagcgg
     1921 gtacgccagt ggcggggctg attatcgtcg gtattttgat gaccatcttc cagctcagca
     1981 gcatttcacc aaacgcgacc aaagaattcg gtctggtttc ttccgtgtcg gtcatcttta
     2041 cactggtgcc atatctttac acctgtgcgg cgttactgct gctcggacac ggtcactttg
     2101 gtaaagcacg cccggcatat ctggcggtta ctaccattgc cttcctctac tgcatctggg
     2161 ccgtggtggg gtccggggcg aaagaggtta tgtggtcatt tgtcaccctg atggtcatca
     2221 ccgccatgta tgcgctgaat tacaaccggc tgcataaaaa cccgtatccc ttagatgcac
     2281 caataagcaa agattaattc cccttaatcc agcaaagata aaagccaacc ttaagaactt
     2341 aaggttggct taattttgct ttgcgagcat atgcgcactt tgttcgatgg aaacaccgtg
     2401 atgttgaagc gcctactaaa aagaccctct ttgaatttac tcgcctggct attgttggcc
     2461 gctttttata tctctatctg cctgaatatt gcctttttta aacaggtgtt gcaggcgctg
     2521 ccgctggact cgctgcataa cgtactggtt ttcttgtcga tgccggtcgt agccttcagc
     2581 gtgattaata ttgtcctgac actaagctct ttcttatggc ttaatcgacc gctggcctgc
     2641 ctgtttattc tggttggcgc ggctgcacaa tatttcataa tgacttacgg catcgtcatc
     2701 gaccgctcga tgattgccaa tattattgat accactccgg cagaaagcta tgcgctgatg
     2761 acaccgcaaa tgttattaac gctgggattc agcggcgtgc ttgctgcgct gattgcctgc
     2821 tggattaaaa tcaaacctgc cacctcgcgt ctgcgcagtg ttcttttccg tggagccaat
     2881 attctggttt ctgtactgct gattttgctg gtcgccgcac tattttataa agactacgcc
     2941 tcgttgttcc gcaacaacaa agagctggtg aagtccttaa gcccctctaa cagcattgtt
     3001 gccagctggt catggtactc ccatcagcga ctggcaaatc tgccgctggt gcgaattggt
     3061 gaagacgcgc accgcaaccc attaatgcag aacgaaaaac gtaaaaattt gaccatcctg
     3121 attgtcggcg aaacctcgcg ggcggagaac ttctccctca acggctaccc gcgtgaaact
     3181 aacccgcggt tggcgaaaga taacgtggtc tatttcccta ataccgcatc ttgcggcacg
     3241 gcaacggcag tttcagtacc gtgcatgttc tcggatatgc cgcgagagca ctacaaagaa
     3301 gagctggcac agcaccagga aggcgtgctg gatatcattc agcgagcggg catcaacgtg
     3361 ctgtggaatg acaacgatgg cggctgtaaa ggtgcctgcg accgcgtgcc tcaccagaac
     3421 gtcaccgcgc tgaatctgcc tgatcagtgc atcaacggcg aatgctatga cgaagtgctg
     3481 ttccacgggc tggaagagta catcaataac ctgcagggtg atggcgtgat tgtcttacac
     3541 accatcggca gccacggtcc gacctattac aaccgctatc cgccgcagtt caggaaattt
     3601 accccaacct gcgacaccaa tgagatccag acctgtacca aagagcaact ggtgaacact
     3661 tacgacaaca cgctggttta cgtcgactat attgttgata aagcgattaa tctgctgaaa
     3721 gaacatcagg ataaatttac caccagcctg gtttatcttt ctgaccacgg tgaatcgtta
     3781 ggtgaaaatg gcatctatct gcacggtctg ccttatgcca tcgccccgga tagccaaaaa
     3841 caggtgccga tgctgctgtg gctgtcggag gattatcaaa aacggtatca ggttgaccag
     3901 aactgcctgc aaaaacaggc gcaaacgcaa cactattcac aagacaattt attctccacg
     3961 ctattgggat taactggcgt tgagacgaag tattaccagg ctgcggatga tattctgcaa
     4021 acttgcagga gagtgagtga atgaaaattc tgattgttga agacgatacg ctgttattgc
     4081 agggactgat tctggcggcg caaaccgaag gctacgcgtg cgatggcgtg acaaccgcgc
     4141 ggatggcgga acaaagcctt gaggcaggtc attacagcct ggtggtactg gatttagggt
     4201 tacccgacga agatggactg cattttctcg cccgtatccg gcagaaaaaa tataccctgc
     4261 cggtactgat cctcaccgct cgcgatacgc tgaccgacaa aatcgccggg ctggatgtcg
     4321 gtgccgacga ctatctggtg aagccttttg cgctggaaga gttacatgcc cgtatccgcg
     4381 ccctgctacg acgccataat aatcagggcg aaagtgagct gattgttggc aatctgacgc
     4441 tgaacatggg tcgccgtcag gtatggatgg gcggtgaaga gttgattctg acgcccaaag
     4501 aatatgctct gctgtcacgg ttaatgctca aagcaggcag tccggtgcat cgggaaattc
     4561 tctacaacga catctataac tgggacaatg aaccctcgac caacaccctg gaagtgcata
     4621 tccacaatct gcgcgacaaa gtgggcaaag cccgtatccg caccgtgcgc ggctttggct
     4681 atatgcttgt cgcgaatgag gaaaactaat tgaatctgat gcgttttctg cgccgaccaa
     4741 tatcgctgcg ccaacggctg atattgacca tcggggccat tttgttggtg tttgagctga
     4801 tcagcgtctt ctggctatgg catgaaagta ccgagcagat tcagctgttt gagcaggcgc
     4861 tacgcgacaa tcgcaacaac gatcgccaca tcatgcgtga gatccgcgag gcggtcgcca
     4921 gcctgattgt ccccggcgtc tttatggtca gcctgacgct atttatctgc tatcaggcgg
     4981 tacgccgcat cacccgcccg ctggcggagc tgcaaaaaga gctggaagcg cgcaccgccg
     5041 acaacttaac gcccattgcc attcacagcg ccaccctcga aatcgaagcg gtggtttcgg
     5101 cgttgaacga tctggtcagt cgcctgacca gcacgctgga taacgaaagg ttatttaccg
     5161 ctgacgtcgc gcacgaactg cgaacgccac tggcgggggt gcgtttgcat ctggaactgc
     5221 tggcgaaaac gcatcacatt gatgtagcac cgttagtggc acggcttgat cagatgatgg
     5281 agagcgtctc ccagctgctg caactggcgc gtgccggaca gtcattttct tccggtaatt
     5341 atcaacatgt aaaactgctg gaagatgtca ttctcccctc gtatgacgaa ctcagtacca
     5401 tgctcgacca gcgacagcaa accctgctat tgccagagag cgccgccgac atcaccgttc
     5461 agggtgatgc aaccctgctg cggatgttat tgcgaaacct ggtagaaaac gcccatcgtt
     5521 acagcccgca aggcagcaac attatgatta agctgcaaga agatggcgga gcggtcatgg
     5581 ccgttgaaga tgaaggaccg ggtattgatg agagtaaatg cggggagttg agtaaagcgt
     5641 ttgtacgtat ggacagccgt tatggcggga ttggtctggg gttaagtatt gtcagccgca
     5701 ttacacagtt gcatcacggg cagtttttcc tgcaaaaccg gcaagagact tccggcacgc
     5761 gggcctgggt acggctgaag aaagatcaga acgtggcaaa ccagatataa agaaagctgc
     5821 tgaccaccag cacgctgaac acggtagtta aactttcata aacacggttt ttcattgcac
     5881 tctcctccgt taacctggag gagagtatgc gcgtcagaga ttaagtgaac cttaagagtt
     5941 caattggcct gataagacag cgttacatca ggccatccgt ttcagcttat tcatcaattc
     6001 gcggatgttg ctgcaccagg cgggtacgtt tcgcctgcaa atcggcaatc tcgtggtcaa
     6061 tatcatcgat tttctgctcg atattatcgt aatgctcgac gagaatttcc ttcgcttcct
     6121 gtatatctga cgccgccggt gttgcacctt tcaacggacg atttgccgtc tctttcatgg
     6181 ttacgccggt gattaaacca accaccgcca ctaccatcag gtaataggct ggcatcatca
     6241 gattctgcga gctttcgacc agccaggccg ccagtgttgg cgtcagaccg gcaaccagca
     6301 ccgaaatatt aaatgccgcc gccagcgcgc tgtaacggat atgcgtcggg aacatcgctg
     6361 gcaaggtaga agccataacg cccgtaaagc agttaaggat caccgccagc atcagtaacc
     6421 cggcaaaaat caggccgatg acgttactgt taatcagaat aaacgccggg atcgccaaca
     6481 caaacagtgc aacactacca agtagcacaa acggacgacg gccaaaacgg tcactcagca
     6541 agcccatcac cggctggaca aacagcatac cgatcataat ggcgataata atcagcaccc
     6601 cgtggtcttc ggagtaatgc aggttatgcg acaaataact cggcatatag gtcagcaaca
     6661 tgtagtaagt cacgttggtg gcaattacca gaccaataca tgtcaacagg ctgcgccagt
     6721 atttagtggc aatctcttta aacgagactt tcgggccatc ctgcaaacct tcgcggtcgc
     6781 cctgttccag tttatcaaca tgctgctgga acgccggagt ttcttccagc gcatggcgca
     6841 ggtaaagccc gataatccct aacggcagag caataaagaa cggaatacgc cagccccagt
     6901 cgaggaagtt cgcttcgccg acaatggtcg aaattaacac caccacgccc gcacccagca
     6961 caaacccggc aatagaaccg aagtccagcc agctgcccat aaagccacgt ttacggtcag
     7021 gggagtattc cgcaacaaat atcgacgccc cggtatattc accgccgacc gagaaacctt
     7081 gtgccatctt acagatcaac agcagaatcg gtgcccaaat accaatcgtg tcgtaggacg
     7141 gtattaagcc aatacagaac gtactgatcg acataatcac aatagtgata gcgaggatct
     7201 tctggcgacc atatttatcg cccaacatac caaagaagag tccgccaagc ggtcgaatca
     7261 gaaagggaac ggagaaagtg gcaagtgcag caaccatctg cacgctgggg tcagcccccg
     7321 ggaaaaaaac tttacctaat gcgtaagcaa caaaaccata aacaccaaaa tcaaaccatt
     7381 ccattgcatt acccagtgat gctgcggtaa tggctttacg cagtttaccg tcatcaataa
     7441 tggtgacatc acgaagggta atcggtttta cttttttcct tttcagcata gctttcctcg
     7501 cagagttggc ccatttccgc aaacgccaga aaaatcagcg cgacggccgc aaaaggggag
     7561 cgggaattgc aggatgcaat ctctgtaaca agcgtagcgg gtttgcttac accctccggt
     7621 taaggaatgt acaatcaaac cagttgacgc ctgaattggg cagttaatga cctttttacc
     7681 ctaccagcgt ttaaatttgt gatcaacttc acacatggat ttcgtttagg actcattgat
     7741 gtaactgatt atttacacca aatttacaga gaagatttcc tcgcgcattg cccccgccag
     7801 acgctgcgct gaacagagtc gtggcatccc cagcgcgaca gtttgccagc gttgggtcac
     7861 tgaccagcaa acgggttgtt tatctgccgt ggtccacttt cccagccaac ttaaaaaatc
     7921 agcatgatcc ccgcccgtcg gaacggtcga agaggcaaac cactgatggt aatagtgacg
     7981 cgtggtcggg gccgcattga tactctctgc cagatgttgt gtcgctttca cgcgcaaatc
     8041 acccagcaac aacgccaccg cttgcgggct tcccagacga caggcatcgc caaatgcccc
     8101 ccaacgcaac tcttcgagaa cgataaaaca gcgcccaggc aacgaccagg cgttatacgc
     8161 cccagcacgc cagcgggtaa aaatttgctc gctctgttca cccgcctgca ccagtaggcc
     8221 tcgttgcgtt aacgcctttt ccttccagcg acaacgggct gaaaaccact catacaaggc
     8281 ggcaatttgc tgggggagct ccgggaaagc agagtgctgg cggtgatcca ttttttgcag
     8341 cagcgccaga tgctgcgcga tctgcgtcag tgccagatga ccgggatcca gcatcgacgc
     8401 cagtttttcc accaatgtca gacgcagact aagcggcgca tttaacatcc cgccgttcgt
     8461 ccatgtctga agctgcacgc gcgcaagaag ttcctgcatc agtcgttcac gaaactgctg
     8521 ctgatgggct tgtggaaggc gggcatcatc gccctgcgcc agatccacta aaaagcgggg
     8581 ataaaccgac tccagcacgc gaccggggcc gtccagtaac gtcttggtca atatcgttct
     8641 gccgtgaaaa gtgtttgaat atcatcgcgt aacagctgag cgtcggtgta aatccagccg
     8701 tgagtcatca cagtctgctg caattgctgt tgcatcagcc tgaccaccga ttcattttgt
     8761 tgacgcagag ccagactttc gcgtaaacgc gtctgtaatt ccgtcaaaca tgaagcgaac
     8821 tcagcgaaaa aagtattcat gcctgccgta acagattcat cgacctgctc tgccagaact
     8881 ttagccattt gttggcaata aagatcgact tctgcgctta atgctcgttg caacacactg
     8941 taatcaaccg tttctgtcgg ggatttctca tttccccgtc cccagtcagg ctgattcaac
     9001 cagcgcgaaa aagtctcacg cacaacgcct aatcgcgtgc tctgctcgtc cgttgcttcc
     9061 tggcgcgaaa tgacttcact gaacagctgg cgagtgttga agtggggaac tacgccgtga
     9121 aaaacaggaa aatgaaaccc aggacgaaac cctgactcgc tcaattccat tttgacttgt
     9181 tgctcaatgg ggcgaataac atcggttaac acccggcaaa gggtggattc aagctcggca
     9241 aaacgcagcg taaagtcgcg actgatggtg ttctgcgccg tctgtaacag tgtctcacag
     9301 cgggtacgaa tctcgcttaa cggctccgaa tcatcctgaa acaaggcggc taactgcacc
     9361 ttcagcgcat cttgctgttg acgcagaaag tggttggcgg aggtcagggc cagctcgatt
     9421 tcatgtttaa tctcgccgct cacctgcgcc tgattgagtt gcaatagctg caaactttct
     9481 tcgatctggt ggatattttg ccgcaattgt tcacaagcga cgtttaaccc gtgcgcacga
     9541 aaatccaggt attcccgcgc ctgctgcgcg taattcaaca gtttatgcgc ggcagatcgc
     9601 agagcataca acgaggcgtt agcgtaagcg gcatgaagca acatctgaat tggctgggcg
     9661 aacagcgaat cttcccacaa ctgatcggca gcatgacgaa tatgttcgag gtccgccaga
     9721 tcggcatgac gccagcgcct gccgagcgcg gcatgggcaa aatcttccac ccagcgttgt
     9781 tgctctggcg gtggtaactt accgttgttg gctaactcat ggcgcgcccg atttgccagg
     9841 tagccccaca tcgacgacac cggaaatatc tgctgtggcg taatacagcc tttcatcagc
     9901 gtcccggaaa tcagtgcccg cacctggtcg gcgtcgtcac tgttacgatc ctgttgatcg
     9961 aacttattga ccagcacata cagcggcacc gattgcccca ccgccaaaat cgcctcacgg
    10021 acctcttcat cggagatcga tttcagttgc gtataatcca gcaccgccag taccgccgag
    10081 gcgcgtgcca gctgctggtt aagcattttt tgcagatgcg gttgcccggc ttcatttggc
    10141 ccgggggtat ccagtaacgt caattgaccg ggataacttt ccagccccgc cagatggaca
    10201 aactccactt caatcacggg aatatgctca atggcggcgt aagcagaaaa aggaaaatcg
    10261 acgtccagcg ccttcgccag tcgcaccaaa tcattcaaac ttttcagaca atgaaaaata
    10321 ggctgggcac ccagataata tttttcgaaa gcgacgccat tttcgatccg ctgcataagc
    10381 gcacgcatat ctttatctat ttccagcaca tcggtcagat gcttaatatc gcaatcacgc
    10441 aggcgctgtt gtaatttttg aattaaacaa tcgattggcg cgacatgtga aaaatgcagt
    10501 accggttcct tttggccggg cgtatggcga ataagcgtcg gcagcgcagt cattgggcga
    10561 ttgcgattag gcagtacctc cgtaccaaca atggcattaa tggtggttga tttccctgct
    10621 ttcatggtac cgacaatcgc cagcaccatt tccagtcggg aaattttacg caactcatta
    10681 ttcagcatcg cgtgacgctc ggcgatatta ggctgactcc agggtaaagc cagttgtggc
    10741 gcatcgtctc cgggtacaga gagaggcatt ttttccagta actgcaactg ttggcgagaa
    10801 agctgtaaca gccgttcagc ctcctgactt aactcataca gggtctgtgt gtacatagaa
    10861 aattcttcct taaagcaaat tttattactt ttattt
//
LOCUS       DAZKFZ010000154        10888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000154 DAZKFZ010000000
VERSION     DAZKFZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..442)
                     /gene="ttdR"
                     /locus_tag="ACHOQW_003492"
                     /gene_synonym="dan"
     CDS             complement(<1..442)
                     /gene="ttdR"
                     /locus_tag="ACHOQW_003492"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP9574515.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDI"
     gene            649..1560
                     /gene="ttdA"
                     /locus_tag="ACHOQW_003493"
     CDS             649..1560
                     /gene="ttdA"
                     /locus_tag="ACHOQW_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP9574516.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1557..2162
                     /gene="ttdB"
                     /locus_tag="ACHOQW_003494"
     CDS             1557..2162
                     /gene="ttdB"
                     /locus_tag="ACHOQW_003494"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP9574517.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            2211..3674
                     /gene="ttdT"
                     /locus_tag="ACHOQW_003495"
     CDS             2211..3674
                     /gene="ttdT"
                     /locus_tag="ACHOQW_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP9574518.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3717..4730)
                     /gene="tsaD"
                     /locus_tag="ACHOQW_003496"
     CDS             complement(3717..4730)
                     /gene="tsaD"
                     /locus_tag="ACHOQW_003496"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9574519.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4968..5183
                     /gene="rpsU"
                     /locus_tag="ACHOQW_003497"
     CDS             4968..5183
                     /gene="rpsU"
                     /locus_tag="ACHOQW_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9574520.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            5294..7039
                     /gene="dnaG"
                     /locus_tag="ACHOQW_003498"
     CDS             5294..7039
                     /gene="dnaG"
                     /locus_tag="ACHOQW_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9574521.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            7234..9075
                     /gene="rpoD"
                     /locus_tag="ACHOQW_003499"
     CDS             7234..9075
                     /gene="rpoD"
                     /locus_tag="ACHOQW_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9574522.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(9154..9660)
                     /gene="mug"
                     /locus_tag="ACHOQW_003500"
     CDS             complement(9154..9660)
                     /gene="mug"
                     /locus_tag="ACHOQW_003500"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP9574523.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            9785..9860
                     /locus_tag="ACHOQW_003501"
     tRNA            9785..9860
                     /locus_tag="ACHOQW_003501"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9818..9820,aa:Ile,seq:cat)
     gene            complement(9914..10678)
                     /gene="nfeF"
                     /locus_tag="ACHOQW_003502"
                     /gene_synonym="yqjH"
     CDS             complement(9914..10678)
                     /gene="nfeF"
                     /locus_tag="ACHOQW_003502"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP9574524.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         2625 a         2908 c         2867 g         2488 t
ORIGIN      
        1 gaatatccag atcaatatta tcctgcacta aatcaatttg ccgatcgaac aattcaaaat
       61 acacctgtaa ctcaggataa ttgcgcataa gttcggtaat ggctggcgca atatggctgc
      121 gcccaaaacc aaaactgcaa ccaatacgaa tcatcccctc cgggcgcgtt ttgatttgcg
      181 tgacgtcatc gaccagtcgc tgatattggg tgaggatttc cagcgcatgt tcgtaacaac
      241 gttggccact ttcagtcagc gccacgccgc gagcggagcg gttcagcagc gttgttgcca
      301 gcgtattttc aaggatttgg atccgcttgg tgacaaatgc aggcgtttgc ccaagcgtcg
      361 ccgctgcagc actaaaactc ccgctgtgga cgatttctac cagtacctgc aagtctttgg
      421 ctaaaggcca gctattcagc atcgttaatt atccgcagtt gtgataagcg cagtgtattt
      481 cgcaaaacat tgccattccc tgctctggcg caatattcat tcttattttc gcgttgattc
      541 acgaactgtt aattacatat tcacggtggc aaaaaatata aaaccacatt tttagtggta
      601 gtttgtggcg gtgaattttt ccagacaaat acaaaaactg gagttgccat gatgagcgaa
      661 agtaataagc aacaggcagt gaataagttg acggagattg tcgctaactt taccgccatg
      721 atttctaccc gaatgcctga tgacgtggtg gataaactaa aacagctaaa ggatgccgaa
      781 acgtcgtcga tggggaaaat tatctaccat acgatgttcg acaacatgca aaaagcgatt
      841 gacctgaatc gtcctgcctg tcaggacacc ggggagatta tgttcttcgt taaagtcggt
      901 tcccgcttcc cactgcttgg cgagctgcaa agcatactca aacaagccgt ggaagaggcg
      961 accatcaaag cgccgctgcg tcacaatgcg gtagaaattt ttgacgaagt aaacaccggc
     1021 aaaaataccg gtagcggcgt accgtgggtc acctgggata tcgtccccga cggtgacgat
     1081 gcggaaatcg aagtttacat ggcaggcggc ggctgcacgc taccaggccg ctcgaaagtg
     1141 ttaatgccgt cagaaggcta cgaaggcgtg gtgaaattcg tcttcgaaaa tatctccacc
     1201 ctcgcagtaa acgcctgtcc accggtactg gtgggcgtgg gcatcgccac ctcggtggaa
     1261 accgccgccg tactctcgcg taaagccatt ttgcgcccga ttggcagccg ccaccccaat
     1321 ccaaaagcgg cagagctgga gctacgcctg gaagaaggac tcaaccgtct ggggattggt
     1381 ccacaagggc tgactggcaa cagttcagtg atgggcgtgc atatcgaatc tgccgcccgc
     1441 catccgtcaa ccatcggcgt tgctgtttct accggttgct gggcgcatcg tcgcggcaca
     1501 ctgctggttc atgccgatct cacctttgaa aatctgtctc acacccggag cgcgttatga
     1561 aaaagatcct gacaaccccg atcaaagctg aagatctgca agatattcac gtcggcgatg
     1621 tgatctatct gaccggtacg ctggtgacct gccgtgacgt ttgtcaccgc cgattgatcg
     1681 aactgaaacg tccgctccct tacgatctca acggcaaagc gattttccac gctggcccca
     1741 tcgtgcgcaa aaacggcgac aaatgggaga tggtctccgt cggtccgaca accagtatgc
     1801 gtatggaaag ttttgaacgt gaatttatcg agcagaccgg cgtgaaactg gtggtcggca
     1861 aaggcggaat ggggccgctg accgaagaag gctgtcagaa attcaaggcg ctacatgtaa
     1921 ttttcccggc aggctgcgcg gtgctggcgg caacccaggt ggaagagatt gaagaagtgc
     1981 actggacaga actcgggatg ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc
     2041 cgctgattgt ctctattgat acccacggca acaacctgat agccgaaaac aaaaagctgt
     2101 tcgccgaacg ccgcgatccc atcgtggaag agatctgcga gcacgtccat tacatcaaat
     2161 aaccctcccg gagaggctcg ccccctctcc tttttcgcag gcataacacg atgaaacctt
     2221 ccactgaatg gtggcgatac cttgcgccgc tggcggtcat cgccattatt gctctaattc
     2281 cggttcccgc agggctggag agtcatacct ggctctactt tgccgttttt actggcgtaa
     2341 tcgttggact gatcctcgaa cccgtgccgg gtgccgtggt ggcgatggtg ggtatctcca
     2401 tcatcgccat actctctccc tggctgctgt tcagcccgga gcagctcgct cagccaggct
     2461 ttaaattcac tgcaaaatcc ctctcgtggg ccgtttccgg tttttctaat tcggttatct
     2521 ggctgatttt cgccgccttt atgtttggca caggctatga aaaaaccggg ctggggcgac
     2581 gtatcgcgct gattctggtg aaaaagatgg ggcatcgcac gctgtttctt ggctatgcgg
     2641 tgatgttctc cgagctgatc ctggcacctg tcacaccgtc caactcggcg cgtggtgcgg
     2701 ggattatcta tcccatcatc cgtaacctgc cgccgctcta tcaatcacaa ccaaacgaca
     2761 gcagttcgcg cagtattggc tcgtacatca tgtggatggg gattgttgcc gactgcgtga
     2821 ccagcgccat tttcctgacg gcgatggctc ctaacttgct gttaattgga ctgatgaaaa
     2881 gcgcatctca cgccacgctg agttggggcg actggttcct cgggatgttg ccgctcagta
     2941 ttttactggt cctgctggtt ccctggctgg cttacgtgct gtacccgcct gtactgaagt
     3001 caggcgatca ggtgccgcgc tgggcagaga cggaactgca ggcaatgggc ccgctctgtt
     3061 cgcgtgaaaa acggatgctg gggctgatgg taggcgcgct ggtgctgtgg attttcggcg
     3121 gtgattatat cgatgccgcg atggtcggtt acagcgtggt ggcgctgatg ctgcttctgc
     3181 gcattatcag ttgggacgat attgtcagta ataaagcggc gtggaacgtt ttcttctggc
     3241 tggcctcgct tatcaccctc gcgaccggac tcaacaacac cggttttatt agctggtttg
     3301 gcaaactgtt agcaggcagc ttaagcggtt attcgccaac gatggtgatg gtggcgttga
     3361 ttgtggtgtt ttatctactg cgctactttt ttgccagcgc cacggcgtat acctccgctc
     3421 tcgcaccgat gatgattgcc gctgcgctgg cgatgccgga aatcccgctg ccggtgttct
     3481 gcctgatggt tggtgcggca attggtctgg ggagcattct tacaccatac gccaccggcc
     3541 ccagtccgat ttactacggc agtggttatc tgccaacggc ggattactgg cgactggggg
     3601 cgatttttgg gctgatattc ctcgtattgc tggtgattac cggcttactg tggatgcccg
     3661 tggtgttgct ttaagtaagg acggtgccgg aagtaactct ccggcacccg atagttttac
     3721 gcagccggta actccgccag cggccagcgc ggacgcacgc taacgccgag atccgccgtc
     3781 gcgcctgctt taaaccgcac cattccggca taggcgatca tcgcgccgtt atcagtacaa
     3841 aattccggac gcgcgtagaa cacttcgccg cggcgttttt tcatcatttc agccagcttc
     3901 gcccgtaacg tgcggttagc actcacgccg cccgccatga ccagtcgctt aaagcccgtc
     3961 tgatccagcg cacgcttaca cttaatcatc aacgtatcga ccaccgcatc ttcaaaggcg
     4021 cgggcgatgt cagcacgcgt ctggtcgtcg gtgccgttgt cacgaatggt atttgccgcg
     4081 aaggttttca ggccggagaa gctgaaatcc agccccggac ggtcggtcat cggacgcggg
     4141 aaaacaaagc gcccggcagt accctgcgcc gccattttcg acagtaacgg tccgccagga
     4201 taatccagcc ccagcagctt cgcggtttta tcaaacgctt caccggcggc atcatcgata
     4261 gactcgccga gcagctcgta ctgaccaatg ccagtcacgc tgattaactg cgtatgaccg
     4321 ccggaaacca gcagcgcaac aaacggaaat tccggcgggt tatcttccag catcggcgct
     4381 aacagatgcc cttccatatg gtgtacaggg atcgccggaa cgttccaggc aaacgccaga
     4441 gaacgcccca cggtcgcgcc aaccagcagt gcgccgacta atccagggcc tgcggtatag
     4501 gccacagcat caatgtcttt tgccgttaaa ccagactcct ttagcgccgc ctggatcaac
     4561 ggtacggttt tacgcacatg atcgcgggag gccagttcag gcacaacgcc cccgtagtca
     4621 gcgtgcaatt tcacctgact atacaattgg ttggctaaca aacctttttc atcgtcgtaa
     4681 atggcgatgc cggtttcatc gcaggaagtt tcaataccca gtacacgcat gacttgtttt
     4741 acctcgcttt attaccgcgc agtgtaggac caatgcgggt tgatgtaaaa ctttgttcgc
     4801 ccctggagaa agcctcgtgt atactcctca cccttataaa agtccctttc aaaaaaggcc
     4861 gcggtgcttt acaaagcagc agcaattgca gtaaaattcc gcaccatttt gaaataagct
     4921 ggcgttgatg ccagcggcaa accgaattaa tcaaaggtga gaggcacatg ccggtaatta
     4981 aagtacgtga aaacgagccg ttcgacgtag ctctgcgtcg cttcaagcgt tcctgcgaaa
     5041 aagcaggtgt tctggcggaa gttcgtcgtc gtgagttcta tgaaaaaccg actaccgaac
     5101 gtaagcgcgc taaagcttct gcagtgaaac gtcacgcgaa gaaactggct cgcgaaaacg
     5161 cacgccgcac tcgtctgtac taattccccg agagcgttgc tctccgatca gaccgagttg
     5221 tagttgtaag gccgtgcttc cgaaaggaat gcgcggctta ttttcgttta tgaattgcta
     5281 aaaatcgggg cctatggctg gacgaatccc acgcgtattc attaatgatc tgctggcacg
     5341 cactgacatc gtcgatctga tcgatgcccg tgtgaagctg aaaaagcagg gcaagaattt
     5401 ccacgcgtgt tgtccattcc acaacgagaa aaccccgtcc ttcaccgtta acggtgagaa
     5461 acagttttac cactgctttg gatgtggcgc gcacggcaac gcgatcgact tcctgatgaa
     5521 ctacgacaag cttgagttcg tcgaaacggt cgaagagctg gcggcaatgc acaatcttga
     5581 agtgccattt gaagcaggta gcggccccag ccagatcgag cgccatcagc ggcaaacgct
     5641 ttatcagttg atggacggtc tgaatacgtt ttaccaacaa tctttacaac aacctgttgc
     5701 cacgtctgcg cgccagtatc tggaaaaacg cggattaagc cacgaggtta tcgctcgctt
     5761 tgcgattggt tttgcgcctc ccggctggga caacgtcctg aagcggtttg gcggcaatcc
     5821 agaaaatcgc cagtcattga ttgatgcggg gatgttggtc actaacgatc agggacgcag
     5881 ttacgatcgt ttccgcgagc gggtgatgtt ccccattcgc gataaacgcg gtcgggtgat
     5941 tggttttggc gggcgtgtgc tgggcaacga tacccccaaa tacctgaact cgccggaaac
     6001 agacattttc cataaaggcc gccagcttta cggtctttat gaagcgcagc aggataacgc
     6061 tgaacccaat cgtctgcttg tggtcgaagg ctatatggac gtggtggcgc tggcgcaata
     6121 cggcattaat tacgccgttg cgtcgttagg tacgtcaacc accgccgatc acatacaact
     6181 gttgttccgc gcgaccaaca atgtcatttg ctgttatgac ggcgaccgtg caggccgtga
     6241 tgccgcatgg cgagcgctgg aaacggcgct gccttacatg acagacggcc gtcagctacg
     6301 ctttatgttt ttgcctgatg gcgaagaccc tgacacgctg gtacgaaaag aaggtaaaga
     6361 agcgtttgaa gcgcggatgg agcaggcgat gccgctttcc gcatttctgt ttaacagcct
     6421 gatgccgcaa gttgatctga gtacccctga cgggcgcgca cgtttgagta cgctggcact
     6481 gccattgata tcgcaagtgc cgggcgaaac gctgcgaata tatcttcgtc aggaattagg
     6541 caacaaatta ggcatacttg atgacagcca gcttgaacga ttaatgccaa aagcggcaga
     6601 gagcggcgtt tctcgccctg ttccgcagct aaaacgcacg accatgcgta tacttatagg
     6661 gttgctggtg caaaatccag aattagcgac gttggtcccg ccgcttgaga atctggatga
     6721 aaataagctc cctggacttg gcttattcag agaactggtc aacacttgtc tctcccagcc
     6781 aggtctgacc accgggcaac ttttagagca ctatcgtggt acaaataatg ctgccaccct
     6841 tgaaaaactg tcgatgtggg acgatatagc agataagaat attgctgagc aaaccttcac
     6901 cgactcactc aaccatatgt ttgattcgct gcttgaactg cgccaggaag agttaatcgc
     6961 tcgtgagcgc acgcatggtt taagcaacga agaacgcctg gagctctgga cattaaacca
     7021 ggagctggcg aaaaagtgat ttaacggctt aagtgccgaa gagcgatcgg gaagcccccg
     7081 acagccgcac tgagaggcag cggcaaatat ataagtacgc cctcgtaatt atcgttggcg
     7141 gtaaacaacc gttggatttc agcgttaacg gctgaaggac atcgggtcaa tcgcccaaca
     7201 ccaacctcat gaaataagtg tggataccgt cttatggagc aaaacccgca gtcacagctg
     7261 aaacttcttg tcacccgtgg taaggagcaa ggctatctga cctatgccga ggtcaatgac
     7321 catctgccgg aagatatcgt cgattcagat cagatcgaag acatcatcca aatgatcaac
     7381 gacatgggca ttcaggtgat ggaagaagca ccggatgccg atgatctgat gctggctgaa
     7441 aacaccgcgg acgaagatgc tgccgaagcc gccgcgcagg tgctttccag cgtggaatct
     7501 gaaatcgggc gcacgactga cccggtacgc atgtacatgc gtgaaatggg caccgttgaa
     7561 ctgttgaccc gcgaaggcga aattgacatc gctaagcgta ttgaagacgg gatcaaccag
     7621 gttcaatgct ccgttgctga atatccggaa gcgatcacct atctgctgga acagtacgat
     7681 cgtgttgaag cagaagaagc gcgtctgtcc gatctgatca ccggctttgt tgacccgaac
     7741 gcagaagaag atctggcacc taccgccact cacgtcggtt ctgagctttc ccaggaagat
     7801 ctggacgatg acgaagatga agacgaagaa gatggcgatg acgacagcgc cgatgatgac
     7861 aacagcatcg acccggaact ggctcgcgaa aaatttgcgg aactgcgcgc tcagtacgtt
     7921 gtaacgcgtg acaccatcaa agcgaaaggt cgcagtcacg ctgccgctca ggaagagatc
     7981 ctgaaactgt ctgaagtatt caaacagttc cgcctggtgc cgaagcagtt tgactacctg
     8041 gtcaacagta tgcgcgtcat gatggaccgc gttcgtacgc aagaacgtct gatcatgaag
     8101 ctctgcgttg agcagtgcaa aatgccgaag aaaaacttca ttactctgtt taccggcaac
     8161 gaaaccagcg atacctggtt caacgcggca attgcgatga acaagccgtg gtcggaaaaa
     8221 ctgcacgatg tctctgaaga agtgcatcgc gccctgcaga aactgcagca gattgaagaa
     8281 gaaaccggcc tgaccatcga gcaggttaaa gatatcaacc gtcgtatgtc catcggtgaa
     8341 gcgaaagccc gccgtgcgaa gaaagagatg gttgaagcga acttacgtct ggttatttct
     8401 atcgccaaga aatacaccaa ccgtggcttg cagttccttg acctgattca ggaaggcaac
     8461 atcggtctga tgaaagcggt tgataaattc gaataccgcc gtggttacaa gttctccacc
     8521 tacgcaacct ggtggatccg tcaggcgatc acccgctcta tcgcggatca ggcgcgcacc
     8581 atccgtattc cggtgcatat gattgagact atcaacaaac tcaaccgtat ttctcgccag
     8641 atgctgcaag agatgggccg tgaaccgacg ccggaagaac tggctgaacg tatgctgatg
     8701 ccggaagaca agatccgcaa agtgctgaag atcgccaaag agccaatctc catggaaacg
     8761 ccgatcggtg atgatgaaga ttcgcatctg ggggatttca tcgaggatac caccctcgag
     8821 ctgccgctgg attctgcgac caccgaaagc ctgcgtgcgg caacacacga cgtgctggct
     8881 ggcctgaccg cgcgtgaagc gaaagttctg cgtatgcgtt tcggtatcga tatgaacacc
     8941 gatcacacgc tggaagaagt gggtaaacag ttcgacgtta cccgtgaacg tatccgtcag
     9001 atcgaagcga aggcgctgcg caaactgcgt cacccgagcc gttctgaagt gctgcgtagc
     9061 ttcctggacg attaatcggt aggccggatc aggcgttacg ccgcacccgg cacctgtccc
     9121 tttgcacaaa cgccaccttt tcggtggcgt tttttatcgc ccacgcacca ccagcgcctg
     9181 gtccagctcg cgatacgctt caaccagttt ctccagtgaa acgcgactta aaccgctggg
     9241 atttggcagc acccaaatct gcgtcgaacc aatgctgagc gtttgtttcc cccactgtgc
     9301 accacgctgg ctgaatccct gttcatatgc ttgtttgccc agaatcgcca acgcttgcgg
     9361 ctgataatct tcaatttttt caatcagctt acgcccacct gcgtgtagct cttgctttga
     9421 aatttcattg gcttgcaccg ttggcctgtc taccagtttg gtgacgccac aacgataatc
     9481 cagcagatgc tgtgcctcct gcggcttcaa ctgatggtcg gtaaacccgg cctgatatat
     9541 caccttccag aagcgatttg ccggatgagc aaagggaaaa ccagtcccgg cggatgaaag
     9601 cccagggttg ataccgcaaa acacgacccg taaccctgga gccaaaatat cctcaaccat
     9661 atttactcct ttctaacatc accaggaaaa gtataacgga ttgaaaatac attgtttata
     9721 aaaacagcag gcgcgcggta atggctggat tgcgacacgg agttacttta taatccgcta
     9781 ccatggcccc ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc
     9841 aagtccagca ggggccacca gatatagcaa aggctgacga gaaatcgtca gcctttttct
     9901 ttttatatat cagttacttt gcgtgccagt aggccgctgc acgtacccgc tgtgggtcat
     9961 actgttccgc ttcaaagcgg cggcttaaat tcttaacgac tttaccttcg ccggttatcc
    10021 agatgaagta atcatcggca gggatttgca tctgcgccag acgcgcatct accgcctgct
    10081 catcatgtgc cagccattcg atattaaaac catcaaggtg cgcgagataa tcctgacagg
    10141 cgttatcccg cacgctaacc agcgcactaa cttgcggttt aacggcaagt ttgctcaacg
    10201 tttccaggcg gcggcgcaat gcgggcattc cggattcatc gcagacatac agctgatacg
    10261 cgtaatcttc cggcaccacc agcgaaccgc gcggacctgc caccgtaagt ttatcgcccg
    10321 gttgcgcctg catcgcccag ccgctggcga ccccaccgtc gtgaataaag aaatcgatcg
    10381 ccagttcatg gcgtagttcg tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc
    10441 cttccggcca gacgatgccc tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt
    10501 gaggaaagaa gagtttgctg tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct
    10561 cgccgccaag gacaatgcgc tgaaaaccgg cgctgatacg ctcaacgcgt agcacagtca
    10621 attcacggaa gcgcagatca ttgcgaacgc gctgcgggta gcggggggag ttattcattg
    10681 ttatcgcctt cgtgatggta atcagatata tctaaataat tttcgcaaat gataatgatt
    10741 gttaatcatg ataaatgcaa gcgatttgta gaactgatat gtctatagtc tgacaagacg
    10801 aaccgcctct tctcaggcat cattactcaa cgccggatgc ggcgtgaacg ccttatccgg
    10861 cctacgtgtg agatgagtcc acatttaa
//
LOCUS       DAZKFZ010000155        10881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000155 DAZKFZ010000000
VERSION     DAZKFZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            257..1597
                     /gene="dcuB"
                     /locus_tag="ACHOQW_003503"
     CDS             257..1597
                     /gene="dcuB"
                     /locus_tag="ACHOQW_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP9574525.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1675..3321
                     /gene="fumB"
                     /locus_tag="ACHOQW_003504"
     CDS             1675..3321
                     /gene="fumB"
                     /locus_tag="ACHOQW_003504"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9574526.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            3444..4073
                     /gene="yjdF"
                     /locus_tag="ACHOQW_003505"
     CDS             3444..4073
                     /gene="yjdF"
                     /locus_tag="ACHOQW_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP9574527.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4212..5633)
                     /gene="melB"
                     /locus_tag="ACHOQW_003506"
     CDS             complement(4212..5633)
                     /gene="melB"
                     /locus_tag="ACHOQW_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP9574528.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(5736..7091)
                     /gene="melA"
                     /locus_tag="ACHOQW_003507"
     CDS             complement(5736..7091)
                     /gene="melA"
                     /locus_tag="ACHOQW_003507"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9574529.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7374..8282
                     /gene="melR"
                     /locus_tag="ACHOQW_003508"
     CDS             7374..8282
                     /gene="melR"
                     /locus_tag="ACHOQW_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP9574530.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            8481..10748
                     /gene="adiA"
                     /locus_tag="ACHOQW_003509"
     CDS             8481..10748
                     /gene="adiA"
                     /locus_tag="ACHOQW_003509"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP9574531.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2774 a         2894 c         2671 g         2542 t
ORIGIN      
        1 aacaaaacat taacatctgc gcagtacaaa ctttaaaccc atcgccagag agtttttctc
       61 tctgaaaaag ccgcttatca cagtgcataa atttgccgct gctttaatca gccaatattc
      121 actgtgaggt atttgctaaa gccggtaacg accaaacgga tatttagtca ggctctgaaa
      181 acagttcata caaaacagaa cgtgactgtg atctattcag caaaaattta aataggatta
      241 tcgcgagggt tcacacatgt tatttactat ccaacttatc ataatactga tatgtctgtt
      301 ttatggtgcc agaaagggtg gtatcgcgct gggtttatta ggcggtatcg gtctggtcat
      361 tctggtcttc gtcttccacc ttcagccagg taaaccacca gttgatgtca tgctggttat
      421 cattgcggtg gtggcggcat cggcgacctt gcaagcttcg ggcggtcttg atgtcatgct
      481 gcaaattgcc gagaagctgc tgcgccgcaa cccgaaatat gtctcaattg tcgcgccgtt
      541 tgtgacctgt acgctgacca ttctttgcgg tacgggtcat gtggtttaca ccattctgcc
      601 gatcatctac gacgtcgcca ttaagaacaa catccgtccg gaacgtccga tggcggcaag
      661 ttctatcggt gcacagatgg ggattatcgc cagtccggtg tcggttgcgg tcgtgtctct
      721 ggttgcaatg ctgggtaatg tcacctttga tggtcgccat cttgagttcc tcgacctgct
      781 ggcaatcacc attccatcga cgttaatcgg tatcctggcg atcggtatct tcagctggtt
      841 ccgcggtaaa gatctggata aagacgaaga gttccagaaa ttcatctccg taccggaaaa
      901 ccgtgagtat gtttacggtg ataccgcgac gctgctggat aaaaaactgc cgaaaagcaa
      961 ctggctggca atgtggattt tcctcggggc aatcgctgta gtcgcacttc ttggtgctga
     1021 ttcggacctg cgtccatcct tcggcggcaa accgctgtcg atggtactgg ttattcagat
     1081 gtttatgctg ctgaccgggg cgctgattat tatcctgacc aaaaccaatc ccgcgtctat
     1141 ctcaaaaaac gaagtcttcc gttccggtat gatcgccatc gtggcggtgt acggtatcgc
     1201 atggatggca gaaaccatgt tcggtgcgca tatgtctgaa attcagggcg tactgggtga
     1261 aatggtgaaa gagtatccgt gggcctatgc cattgttctg ctgctggttt ccaagtttgt
     1321 aaactctcag gctgcggcgc tggcggcgat tgttccggtc gcgctagcga tcggcgttga
     1381 tccggcatac atcgtggctt cagcaccggc ttgctacggt tattacatcc tgccgactta
     1441 tccgagcgat ctggcagcga ttcagtttga ccgttccggc accacccaca tcggtcgctt
     1501 cgtcatcaac cacagcttta ttctgccggg gttgattggt gtgagcgtat cgtgcgtctt
     1561 cggctggatc ttcgccgcga tgtacgggtt cttataaatg cactttgcgt gccgcccgtt
     1621 actacgcggc acgccatttt cgaataacaa atacagagtt acaggctgga agctatgtca
     1681 aacaaaccct ttatctacca ggcacctttc ccgatgggga aagacaatac cgaatactat
     1741 ctactcactt ccgattacgt tagcgttgcc gacttcgacg gcgaaaccat cctgaaagtg
     1801 gaaccagaag ccctgaccct gctggcgcag caagccttcc acgacgcttc tttcatgctc
     1861 cgtccggcac accagaaaca ggttgcggct attcttcacg atccagaagc cagcgaaaac
     1921 gacaagtacg tggcactgca attcttaaga aactccgaaa tcgccgccaa aggcgtgctg
     1981 ccgacctgcc aggataccgg caccgcgatc atcgtcggta aaaaaggcca gcgcgtgtgg
     2041 accggtggcg gtgatgaaga agcgctgtcg aaaggcgtct ataacaccta tatcgaagat
     2101 aacctgcgct actcgcaaaa cgcggcgctg gacatgtaca aagaggtcaa caccggcact
     2161 aacctgcctg cgcaaatcga cctgtacgcg gtagatggcg atgagtacaa attcctttgc
     2221 gttgcgaaag gcggcggctc tgccaacaaa acctatctct accaggaaac caaagccctg
     2281 ctgactcccg gcaaactgaa aaacttcctc gtcgagaaaa tgcgtaccct cggtactgca
     2341 gcctgcccgc cgtaccatat cgcgtttgtg attggcggta cgtctgtgga aaccaacctg
     2401 aaaaccgtta agttagcaag cgctcactat tacgatgaac tgccgacgga agggaacgaa
     2461 catggtcagg cgttccgcga tgtccagctg gaacaggaac tgctggaaga ggcccagaaa
     2521 ctcggtcttg gcgcgcagtt tggcggtaaa tacttcgcgc acgacattcg cgttatccgt
     2581 ctgccacgtc acggcgcatc ctgcccggta ggcatgggcg tctcctgctc cgctgaccgt
     2641 aacattaaag cgaaaatcaa ccgcgaaggt atctggatcg aaaaactgga acaccatcca
     2701 ggtcagtaca ttccacaaga actgcgccag gccggtgaag gcgaagcggt gaaggtcgac
     2761 cttaaccgcc cgatgaaaga gatcctcgcc cagctttcgc aatatccggt ttccactcgt
     2821 ttgtcgctca ccggcaccat tatcgtgggc cgagatattg cacacgccaa gctgaaagag
     2881 ctgattgacg ccggtaaaga acttccgcag tacatcaaag atcacccgat ctactacgcg
     2941 ggtccggcga aaacccctgc cggttatcca tcaggctcac ttggcccaac caccgcaggt
     3001 cgtatggact cctatgtgga tctgctgcaa tcccacggcg gcagcatgat catgctagcg
     3061 aaaggtaacc gcagtcagca ggttaccgac gcgtgtcata aacacggcgg cttctacctc
     3121 ggtagcatcg gcggtccggc ggcggtactg gcgcagcaga gcatcaagca tctggaatgc
     3181 gtcgcttatc cggagctggg tatggaagct atctggaaaa tcgaagtaga agatttcccg
     3241 gcgtttatcc tggtcgatga caaaggtaac gacttcttcc agcaaatcat caacaaacag
     3301 tgcgcgaact gcactaagta acctcttcgg cccagcgcct ggcagcatgc tgccaggtga
     3361 tccccctggg ccacctcttt tgcgattgta atttcacgct tgctggtgaa tagtcagtat
     3421 tttccctgac ttgcgaactc accatgaccc gcacactcaa gccgttaatt cttaacaccg
     3481 gcgcactggc gctaacgtta atcctgattt ataccggcat ttcggcccat gacaaactca
     3541 cctggctgat ggaagtgaca ccggtgatta ttgtcgtgcc gctactgctt gccaccgcta
     3601 aacgttatcc gttaacgccg ctgctctata cgctcatttt ctttcacgcc atcatcctga
     3661 tggtcggcgg gcaatacacc tacgcgaaag tccccatcgg ttttgaggtg caggaatggc
     3721 tggggttgag ccgtaatccg tatgacaagc tggggcattt tttccagggg ctggtgcctg
     3781 cactggtggc acgagagatc cttgtgcgcg ggatgtacgt tcgcggacgt aaaatggtgg
     3841 cgtttctggt ctgttgcgtg gcgctggcga taagcgcgat gtatgaatta atcgagtggt
     3901 gggcggcgct ggcaatgggc cagggagcgg atgattttct cggcacccag ggcgaccagt
     3961 gggacacgca atccgatatg ttctgcgcgc tgcttggcgc attaaccacg gtgatattcc
     4021 tcgctcgctt tcattgccgt cagctacggc gctttggctt gataaccgga taacaacgtg
     4081 cactttgttg atgccggatg cggcgtgaat tccttatccg gtctacaaaa tttgcaaatc
     4141 caaagcattc cattaagacg tggcctgata aacatatcgc atcaggccat tttcgttagc
     4201 tattgtccac gtcaggcttt cacatcactc actgcaccga ccggaatatc agcatgaaca
     4261 ggctcgggcg gtattttgcg atatttatcc agcagatgaa tctggatcct gcgcagcgtg
     4321 tcgccattga ggcgatagaa acggaagtag agaatcagcg ttaccatgaa aaacagagtc
     4381 ggtagagcaa tcataataaa ctgcatacct aatagggctt gcgtagactg ttcaacattc
     4441 ggtacatagc caatcatccc taacacaacc gcaatgaaaa aagccgcaaa ggctgaaccg
     4501 cccttcacca ccatagtctg cacggagtaa gcgatgcttt cacagcgtac gtgcagttta
     4561 tattcaccgt aatcaacggt atctgccacc atgatgacct gtaataccca gaaaagcgcc
     4621 gttcccacat tcagcaaaat acccgcaatc acaatgagga cgacgttgtg atagctcatt
     4681 aatgccatta acaggagaac accacagctt aacaccggaa gaatagatgc tccggcccat
     4741 aaaatgcgtc gggataatga tttaactaag cgtgggaaga atactaacgt caccaggtta
     4801 gcagctcccg catacgacag ataatagggg aacaaatccg catcaccgat aacatatgag
     4861 aaataataga tagcaaagcc ggtaataatg ttgctggcta cattataagc aagagccata
     4921 cccaagaggc atgaaagctg atcgttttta taaataagcg caacgatggc tttaagtgtc
     4981 agatggcttc cttcagcaga cggttgattg tctgacgaaa agacttcatg cacattgcgc
     5041 agagtgatga tggttgaaac aataaaaaag gcgatcagta ccagtgtgaa catctgaaag
     5101 ccaaatcccc gatcgccacc gccgacataa ttaacaaatg gtagcgtcac acctgccgta
     5161 acaaagcccg ccagactggc aaaaaaacgc ggataaggaa ccagttgttc acgctcacgt
     5221 ttatcgagcg tgatggttgg aaccagcgac cagaagggaa tatccataat ggtgtaagtc
     5281 atgccccaga ggatgtaggt cacgcaaaca aaggcaatct gagtagtacc ttcaaacaga
     5341 tgcgcactaa agaggagaaa taagattaca gagtttgcca acgtaccgat caggatccag
     5401 ggtttgaact taccccatcg cgatcgcgta gcatttacaa tccatcccat aatcggatcg
     5461 ttaatagcat cccagatcct cgccaccaga aacaaagtac cgaccaaacc cacagacagc
     5521 ccgacgacat cggtgtagta atacatgagg tacatataca caatgccgat cgcaaaatcc
     5581 ttcccgaacg ctccaaatcc ataactgagt tttgtagtca ttgaaatgct catagggtat
     5641 cgggtcgctg ttaccaacgc ccgccttctg ttattggtat cgtcagaggt gtgcgccgga
     5701 ataaatcccc cggcgcagta gcgtttagtc gcgttttaac ggtgcaacca gcctggcagc
     5761 cagtcgccgt gggcggcaat caggtcgtca acaagagcat atatttcgtc aatgcccagc
     5821 acggcggcag tatgcgggtc catcatcgca gcgtggtaaa cacggtcgcg attttccgta
     5881 agaatagctt cggtcagcag cgtctgtacg ttgatgttgg tttgcatcag ggcggccaga
     5941 tgcgaaggta gcgtaccgac tttggtcggc tgaatgccat tagcatcaac cagacaggcc
     6001 acttccacgc aacatccttg tggcaggtta tcaatcaaac catcgttacg gacgttgccg
     6061 taaatcacac tcggctcgcc cgtccagata gcgttcatga ttgtgctggc atattcccgt
     6121 gacggtttaa tatcaatccg ggaggcgttt ttatactcct ccagctcttt atgccagttc
     6181 gccagctgct cgacgcagcg tttcgggtac tcatccagcg gtactttata acgctcaatc
     6241 aaatcctcac gacctggctt aataaaccac ggtgtgtact cagcaaaatg ttctgacgat
     6301 tccgtgacga aatagcccag ctttttgaac atttcatagc gcacaatatt ctggcagcga
     6361 gtattgccgt gaatattcgg cttcggtgcc tgccctgcgt cataagccgc cagcagttcc
     6421 gggtagagat tcacgtaact gccgtcggcg gttttgcgct ccagctccag gtaaaacgcc
     6481 atatggttga tacctgcgca acggtaacgc agcgtagccg ggtcgatatt gaggtcacgc
     6541 gccagctctt ccgccgttcc ctgcaccgaa tggcacagcc cgacctgttt gatatgcgga
     6601 tagcgggcat acatcgccca ggtattcatc gccattgggt taacatagtt gagcatggtg
     6661 gcatcggggc agacttccgt catgtcctcg caaatttgcc acagatgcgg aatggtacgt
     6721 agcgcgcgca taataccgcc cggccccaac gtatcggcaa tggtttgttc cagaccatgc
     6781 cgcttacaga cctcgaaatc agtcaccgtg caaggtgcat aaccgccaat ctgaaatgcc
     6841 accacgacaa aatcggcatc ctgtaaggct tctttctgtt gggtgtggca ggtgattttg
     6901 ccgctggccc ctgctgaatc catcagctta cgcaccacaa tatgcgactc ttccaggcgg
     6961 gtggggtcaa tgtccatcag ggcaatatgc gccgttttca gcgcctcgcg atggaacaca
     7021 tcaccaagaa tatttttaac gaaaatcgtc gaaccagcgc cgataaatgt aattttgggt
     7081 gcagacatca tgcagatctc ctggcttgct tgaataactt catcatggca ggcgaatctg
     7141 cagaaaacct ccggcttcca ggttgagcat tcccataaac tcagatcctg cgtgaagcag
     7201 cagtaaatct gagtttatgg gaataattta cctgaaaagc agagttttcg gcctcatgat
     7261 gctcggacac tcccctcgcc cctctgggaa gagggtgaaa accgtgctcc cactcgcagt
     7321 catcctccct cactcctgcc ataattctga tattccagga aagagagcca tccatgaata
     7381 cagatacgtt tatgtgcagc agcgacgaaa aacagacccg cagcccgctg tcgctgtatt
     7441 cggaatacca gcggatggag attgagtttc gcgcaccgca tatcatgccc accagccact
     7501 ggcatggtca ggtcgaagtg aatgtgcctt tcgatggcga tgtggaatac ctgatcaaca
     7561 atgaaaaagt gaatatcaat cagggacata tcacgctgtt ctgggcctgt ataccgcacc
     7621 aactaacaga taccggaacc tgtcagagca tggcgatttt taatctgccg atgcatctgt
     7681 ttctctcctg gccgctggat aaagacctga ttaaccacgt cactcacggc atggtgatca
     7741 aatcacttgc tacacagcaa cttagcccgt ttgaagtgcg ccgctggcag caggaattaa
     7801 acagtccgaa tgagcaaatt cgccagctcg ccattgatga aattggcctg atgctcaagc
     7861 gatttagcct ctctggctgg gaaccgattc tggtcaataa aacctcgcgc acacacaaaa
     7921 acagcgtctc gcgccatgcg caattttatg ttagccagat gctgggcttt attgccgaaa
     7981 actatgacca ggcgctgacc atcaacgatg tggctgagca cgtcaaactt aacgccaact
     8041 atgcaatggg gatattccag cgggtcatgc aattgacgat gaaacagtac attaccgcga
     8101 tgcgcatcaa ccacgttcgc gcgttactga gcgataccga taaaagtatt ctcgatattg
     8161 ccctgacggc aggctttcgt tcaagtagtc gtttttacag cacgttcggc aaatatgtcg
     8221 gcatgtcgcc gcaacaatac cgcaaactta gccaacagcg ccgccagacg tttcccggct
     8281 aaagacgata tcagtatcag ccaaaaaaat agtttagccg tcgataataa gcagtgaaaa
     8341 taatttaaaa taatcacata acatattgtt tataaaaaca aatatttcaa aaatgtttgt
     8401 ttttcacgcg ctttacagcc cgaaaaggcc ggaagatact tgcccgcaac gaagattcct
     8461 tcataaccgg gtaagcaatg atgaaagtat taattgttga aagcgagttt ctccatcaag
     8521 acacctgggt cggtaacgct gttgagcgtc tggcagatgc tttaagccag caaaatgtta
     8581 ccgtgattaa atccacctcc tttgatgatg gttttgccat tctctcttca aacgaagcca
     8641 ttgactgcct gatgttcagc tatcaaatgg aacatccgga cgaacatcaa aacgtcagac
     8701 aattgatcgg taagcttcat gagcgccaac aaaacgtgcc ggtcttcctg ttgggcgatc
     8761 gggaaaaagc cctcgccgca atggaccggg atctgctgga gcttgtcgat gaattcgcct
     8821 ggattctgga agataccgcc gactttatcg ccggacgcgc cgttgccgcg atgacccgct
     8881 accgccagca gctgttgccg ccactgttca gcgcgctgat gaaatatagc gacatccatg
     8941 aatattcctg ggcagcgcca ggccaccagg gcggcgttgg ttttaccaaa acacccgccg
     9001 gacgtttcta ccatgactac tacggtgaaa atctgttccg caccgacatg ggcatcgaac
     9061 gaacttccct cggttctttg cttgaccata ctggcgcatt tggcgaaagc gaaaaatatg
     9121 ccgcacgcgt atttggtgcc gatcgctcct ggtcggtagt cgtcggtact tccggctcta
     9181 accgcaccat catgcaggct tgcatgaccg ataacgatgt cgtggtcgtt gaccgtaact
     9241 gccataaatc catcgaacaa ggtttgatgc tgacaggcgc gaaaccggtc tacatggtgc
     9301 caagccgcaa ccgctacggc attatcgggc caatctatcc gcaggaaatg caacctgaaa
     9361 ccttgcagaa gaaaatcagt gaaagcccgc tgactaaaga caaagccggg caaaaaccgt
     9421 cttactgcgt ggtgaccaac tgcacctatg acggcgtgtg ttataacgct aaagaagcgc
     9481 aggatctgct ggaaaaaacc tccgatcgtc tgcactttga cgaagcctgg tacggctatg
     9541 cacgtttcaa cccgatctat gccgatcact atgccatgcg cggcgaacct ggcgatcaca
     9601 acggtcctac cgttttcgcc acccactcca cccacaaact gctgaatgcg ctttcacagg
     9661 cctcttatat tcatgtacgt gaaggtcgtg gggcgattaa cttctcccgc ttcaaccagg
     9721 cctacatgat gcatgccacc acctccccgc tgtatgccat ctgcgcatcc aacgacgtgg
     9781 cggtgtcgat gatggacggc aacagcggcc tgtcactgac gcaggaagtg atcgacgaag
     9841 cggttgattt ccgtcaggcg atggcgcggc tgtataaaga gttcgccgct gacggtagct
     9901 ggttcttcaa accgtggaac aaagaagtcg tcaccgaccc acaaaccggc aaaacctatg
     9961 actttgctga cgcaccaacc aaactgctga ccaccgttca ggactgctgg gtaatgcatc
    10021 cgggcgaaag ctggcacggc ttcaaagata ttccggataa ctggagtatg ctcgacccga
    10081 ttaaagtcag catccttgct ccgggaatgg gtgaagatgg tgaactggaa gaaaccggtg
    10141 ttccggcggc gctggtcact gcctggcttg gtcgccacgg cattgtgcct acccgcacca
    10201 ctgacttcca aattatgttc ctgttctcta tgggcgtaac ccgtgggaaa tgggggactc
    10261 tggttaacac cctttgctcc ttcaaacgcc actatgacgc caacacaccg ctggcgcagg
    10321 tgatgccgga acttgttgaa caatatcctg acacttacgc gaacatgggg attcacgatc
    10381 tgggtgacac catgtttgcc tggctgaaag aaaacaaccc tggcgcacgg ttgaacgaag
    10441 cctattccgg cctgccggtg gcggaaatca ccccgcgtga agcgtacaac gcgattgtcg
    10501 acaacaatgt cgaactggta tccattgaaa atctgccagg acgtatcgcg gcaaactcag
    10561 ttatcccgta tccgccagga atcccgatgc tgctgtctgg tgaaaacttc ggcgataaaa
    10621 acagtccgca agtaagttat ttacgctcgc tgcaatcctg ggaccatcat ttccctggat
    10681 ttgaacacga aactgaaggg actgaaatta ttgacggtat ttaccacgtt atgtgtgtga
    10741 aagcgtaacc actattccgc tgaaggcgta attgtttaaa taacattacg ccgcctggcc
    10801 ttaggccttt tgagtatggc aacgttttca taaaaattgc tgcaaacaaa aatgtcatac
    10861 tttttgcgcg gccccacccc g
//
LOCUS       DAZKFZ010000156        10877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000156 DAZKFZ010000000
VERSION     DAZKFZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(166..1830)
                     /gene="glnS"
                     /locus_tag="ACHOQW_003510"
     CDS             complement(166..1830)
                     /gene="glnS"
                     /locus_tag="ACHOQW_003510"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP9574532.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2033..3979)
                     /gene="nagE"
                     /locus_tag="ACHOQW_003511"
     CDS             complement(2033..3979)
                     /gene="nagE"
                     /locus_tag="ACHOQW_003511"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP9574533.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4312..5112
                     /gene="nagB"
                     /locus_tag="ACHOQW_003512"
     CDS             4312..5112
                     /gene="nagB"
                     /locus_tag="ACHOQW_003512"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9574534.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5172..6320
                     /gene="nagA"
                     /locus_tag="ACHOQW_003513"
     CDS             5172..6320
                     /gene="nagA"
                     /locus_tag="ACHOQW_003513"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9574535.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6329..7549
                     /gene="nagC"
                     /locus_tag="ACHOQW_003514"
                     /gene_synonym="nagR"
     CDS             6329..7549
                     /gene="nagC"
                     /locus_tag="ACHOQW_003514"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP9574536.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7597..8349
                     /gene="nagD"
                     /locus_tag="ACHOQW_003515"
     CDS             7597..8349
                     /gene="nagD"
                     /locus_tag="ACHOQW_003515"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP9574537.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8606..10270
                     /gene="asnB"
                     /locus_tag="ACHOQW_003516"
     CDS             8606..10270
                     /gene="asnB"
                     /locus_tag="ACHOQW_003516"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP9574538.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10650..10726
                     /locus_tag="ACHOQW_003517"
     tRNA            10650..10726
                     /locus_tag="ACHOQW_003517"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10684..10686,aa:Met,seq:cat)
     gene            10737..10821
                     /locus_tag="ACHOQW_003518"
     tRNA            10737..10821
                     /locus_tag="ACHOQW_003518"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10771..10773,aa:Leu,seq:tag)
BASE COUNT         2707 a         2821 c         2756 g         2593 t
ORIGIN      
        1 gtgaacgcct tatccggcct acaaaagcac gcaaactcaa tatattgcag agatcatgta
       61 ggcctgataa gcgtagcgca tcaggcaatt tagcgtttga aactgtttgt ctgttccttg
      121 ccggatgcga cgtaaacgcc ccatccggca tagcgaaact taaaattact cgcctacttt
      181 cgcccaggta tcacgcagcc caacggtgcg gttaaatacc ggtttttccg ccgtagaatg
      241 gcggctatcg aggcagaagt aaccttcacg ctcaaactgg aatgctttac ccgcaaccgc
      301 atctttcagc gacggttcag caaagccctg tttgatcacc agcgattccg ggttaatcac
      361 cgacaggaaa tcatccgcag cacctgggtt cggcacgctg aacagacggt catacaaacg
      421 gatttcaacc ggcagcgcat gtgccgcgct cacccagtga ataacacctt tgactttacg
      481 accatctgcc ggatctttgc ttaaggtatc ggcgtcataa gtacagaaga tggtggtgat
      541 attaccttcg gcatctttct cgacgcgttc tgccttaatc acataagcat tacgcagacg
      601 cacttcttta cccagcacca gacgtttgta ctgcttgtta gcttcttcgc ggaaatcgga
      661 gcgatcaatc caaatctcac cgctaaacgg cacctgacgg ctccccattt ccggtttgtt
      721 cggatggttc ggcatggtaa ccatttcgcc ttcgccctga tagttttcga taaccagttt
      781 caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg ttgagatctt cacggatgca
      841 ggattccagc gacgccatct caatggtgtt gtcctgcttg gtcacgccga tgcgtttgca
      901 gaactcacga atagaagccg cagtgtaacc acgacgacgc agaccggaaa tggtcggcat
      961 acgcgggtca tcccagcctt caacgtgctt gtcggtcacc agcaagttca gcttacgctt
     1021 ggacatcaca gtgtattcca gattcaggcg cgagaattcg tactggcgcg ggtgaacagg
     1081 aatggtgatg ttgtccagta cccagtcgta cagacgacgg ttatcctgga actcaagcgt
     1141 acacagagag tgcgtaatac cttccagggc atcgctgatg cagtgggtga agtcgtacat
     1201 cgggtagatg caccacttgt tgccagtctg gtggtgttca gcaaatttaa tgcggtacag
     1261 caccggatcg cgcatcacga taaacggtga agccatgtcg attttcgcac gcaggcaggc
     1321 tttaccttct tcaaaaccgc cggtacgcat tttctcgaac agcgccaggt tctcttcaac
     1381 gctacggtcg cggtacgggc tgtttttacc aggctgcgtc agggtgccgc ggtattcgcg
     1441 gatctgttcc ggcgtcagtt catcaacgta cgccaggcct ttattgatca gttcgatcgc
     1501 ataggcgtgg agctgatcaa aataatcgga ggagtaacgg acgttaccag accagtgaaa
     1561 acctaaccac tcaacgtcgt ttttgatcga atcgacatac tcgatatctt cttttaccgg
     1621 gttagtgtcg tcgaaacgca ggttgcactg gcctttatag tcctgggcga tcccgaagtt
     1681 caggcagata gatttcgcat ggccaatatg cagatagcca ttcggctccg gcgggaaacg
     1741 ggtgtgtact gtggtgtgct taccactggc cagatcttca tcgatgatct gacggataaa
     1801 gttagtcggg cgggcttctg cctcactcat cgtggattcc tcaaagcgta aacaacgtat
     1861 aacggcgtat gatcttataa gcgggacagg ctgacaactg ttagttagtg aaaatcgaaa
     1921 atctcactga gattatgggg atgattgatg aaaaaaaaag cggcagagaa gatctccacc
     1981 gcttgtcatt gttggatgcg acgctcaagc gtcgcatcag gcataaagca gattactttt
     2041 tgatttcata cagcggtgtt tgacccgcca caacatggcc ctgagcttta atgatcaagc
     2101 cactgaaatc gtcgatattg ctgcaaacca ccgggctaat catcgaacgg gcgttagcgt
     2161 tcaggtaatc cagatccatt tccagaatcg gttgccctgc gcttacctgc gcgccctctt
     2221 ccaccagacg tttaaagcct ttaccttcca gcgctacggt atcgataccc atgtgaacga
     2281 caatctccgc gcctttttcg gtttccaggc agaacgcgtg gttggtgttg aagattttca
     2341 cgattgttcc tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt
     2401 cacccaccgc tttgctggcg aatgcttcgt caggaacctg atccagtgcc acgacatcac
     2461 cggtaatcgg cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg
     2521 ctacaggagc ggcagttgcc ggagttgctt cagcagaggc agctgctacc gggccacggg
     2581 caacgacttt cttcatcgca tcgccgatgg attctgcttt cgcgccaaca atcacctgaa
     2641 tagtctgttt gttcagtttt actaccccag aagcccccag acgtttacac atcgtatcgt
     2701 taacgcgggc agagtcagcc acggtaaggc gcagacgggt gatacaggcg tcaattgctt
     2761 tcaggttgtc agtgccgcca accgcagcaa tatagttggt cgccagttga ttgaggcctt
     2821 cttcagtgtt gctgttggct tcttcagtaa cgatctcgtc ttctttatct tcacgacccg
     2881 gcgttttcag gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga
     2941 agacaacacc cataaccagc agcatccaga cgttctggct ggcggccggc aggttataca
     3001 tcaacgcgta gtcgatagcc cccgcagaga aagagaagcc ggcatggata cccagcagcg
     3061 ttgccacaaa caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggagcaa
     3121 ggaacatgaa caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa
     3181 cagaaagcag catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg
     3241 ccagcgccgc acccggcagg ccgaacatca tgatcgggaa gaagccggac atgaacatcc
     3301 ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc gtacccgccg
     3361 cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg
     3421 ttgggatcaa cagacggttg atgaaaccaa agataccgga acccagcgcg cccgcagaaa
     3481 cgatccactc gccgcctgca tggatagcgt gctgtaccgg cggccagacg taaccaaaaa
     3541 tggccgccag caccagacag aagaagccgg tggcgatcgg cacaaagcgt ttgccgccga
     3601 agaagctcag gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa
     3661 ccagaccggt aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca
     3721 tcgctttggt taacacaaag taacctaccg cccccgccag cgccgccgca cctgcgctgt
     3781 ctttcgacca gctggatgcc acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg
     3841 caccgcccgc ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca
     3901 acagtgccgc caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga
     3961 aaaaacctaa aatattcatc ttattccccc tacgagaacc ctatttggct cgtttaaagc
     4021 cgtattttta ttgtgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca
     4081 tctgccacag agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgagt gtcttttgtg
     4141 agttttgtca ccaaatatcg ttatcatcac tcccttttac tggctaaacc agaaaactta
     4201 ttttatcatt caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct
     4261 taaagatgcc taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg
     4321 atccccctga ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt
     4381 atcaatgcgt tcaaaccgac tgctgatcgt ccgtttgttc tgggcctgcc aactggcggc
     4441 acgccaatga ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt
     4501 aagcacgttg tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga gcatccggaa
     4561 agctactaca gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac
     4621 atcaaccttc tcaacggcaa cgccccggat atcgacgccg agtgccgcca gtatgaagaa
     4681 aaaatccgtt cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat
     4741 attgcattta acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact
     4801 catgacactc gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa
     4861 tatgccctga ctgtcggtgt tggtacactg ctggatgccg aagaagtgat gattctggtg
     4921 ctgggtagcc agaaagcact ggcgctgcag gccgcagttg aaggttgcgt gaaccatatg
     4981 tggaccatca gctgtctgca actgcatccg aaagccatca tggtgtgcga tgagccttcc
     5041 accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc
     5101 aaaggtctgt aattgttatc cctgccctgg ctccttgctc agggcaatat tttttaaaat
     5161 cgggggtcag aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg
     5221 atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac
     5281 tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg
     5341 atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg
     5401 tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc
     5461 cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt
     5521 acctggcaaa acatccgaat caggcgttag gtctgcatct ggaaggcccg tggctgaatc
     5581 tggtgaaaaa aggtacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg
     5641 atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg gaaatggttc
     5701 ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca
     5761 acgcgacgtt gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc
     5821 tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg
     5881 atgaagctga catttattgc ggtattatcg ctgatggcct gcatgttgat tacgccaaca
     5941 ttcgtaatgc taaacgcctt aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc
     6001 cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaacg
     6061 gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg
     6121 tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt atggcgacgc
     6181 tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag
     6241 tagccaacct gaccgcattc acacctgatt ttaaaatcac caagaccatc gttaacggta
     6301 acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa
     6361 tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg
     6421 gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca gcgtaaccaa
     6481 aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac
     6541 cgggggccgc cgcgctatct ctatcgtcac cgaaacccgc aatttccacg caatcggcgt
     6601 acggcttggt cgtcacgacg ccaccatcac cctgtttgat ctcagcagca aagtgctggc
     6661 agaagaacat tacccgctgc cggaacgtac ccagcagacg ctggaacatg ccctgctgaa
     6721 tgccattgct cagtttattg atagctacca gcgcaaactg cgcgagctga tcgccatttc
     6781 cgttatcctg ccaggacttg ttgacccgga cagcggcaaa attcattaca tgccgcatat
     6841 tcaggtagaa aactgggggc tggtagaagc actggaagag cgttttaaag tgacctgttt
     6901 cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg caagtcagga
     6961 ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa
     7021 cgggcgcatt tttattggcc gcaacggcaa cgtcggtgaa attggccata ttcaggtcga
     7081 accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgctgccaa
     7141 cgccgctatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc
     7201 gctggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagcctggc
     7261 ctcggaagtg attgagtatg tcggtcgtca tctgggcaaa accatcgcca ttgctatcaa
     7321 cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg ataaagtgct
     7381 gctccctgct attgaaagct gcattaatac tcaggcgctg aaggcgtttc gtactaatct
     7441 gccggtggta cgttctgagc tagaccaccg ctcggcaatc ggtgcttttg cgctggtaaa
     7501 acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat
     7561 agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc
     7621 gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg
     7681 cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc
     7741 gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtacctga cagcgtgttt
     7801 tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa gaaagcgtat
     7861 gttgtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat
     7921 gtgaaccctg attttgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat
     7981 aaagcggcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac
     8041 gggcgcggtt tttatccggc ctgtggcgcg ttgtgtgcag ggattgagaa aatctccggg
     8101 cgcaaaccgt tctatgttgg taagccgagt ccgtggatta tccgcgcggc attaaacaaa
     8161 atgcaggcac attcggaaga aacggtgatt gtcggcgata acctgcgtac cgatattctg
     8221 gccggcttcc aggcaggtct ggagacgatt ctggtgcttt ctggtgtttc gtcgctcgac
     8281 gatatcgaca gtatgccttt ccgccccagc tggatttacc cgtcagtcgc tgaaatcgac
     8341 gttatctgaa tagcaaccac gtctcaggac gtggtttctt atttttaccc atcattaaaa
     8401 tcatcccatt caataaaacc cgccaaaaat tatcgatcca tcaataaata catcaccgca
     8461 ccctcccctt tttcacaaat cagcaatcac cattacgttt tttatttttt caccgccaaa
     8521 tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac aacaagcaac
     8581 aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg atatcaaaac
     8641 agacgcagtt gagctgcgta agaaagcact cgagctgtca cgcctgatgc gtcatcgtgg
     8701 cccggactgg tccggtattt atgccagcga taaagccatt ctcgctcacg aacgtctgtc
     8761 aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa cccacgtact
     8821 ggcggtaaac ggtgaaatct acaatcacca ggcattgcgc gctgaatatg gcgatcgtta
     8881 tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc
     8941 ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca gcgaaaaaga
     9001 tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg gctatgacga
     9061 acacggtcag ctgtatgtgg cctcagaaat gaaagccctg gtgccagttt gccgcacgat
     9121 taaagagttc ccggcgggga gctatttgtg gagtcaggac ggcgaaattc gttcttatta
     9181 tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca aaaacgagct
     9241 gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc cttacggtgt
     9301 gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga aatacgcagc
     9361 ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccgcagttac actcctttgc
     9421 tgtaggtctg ccgggttcac cggatctgaa agcagcccag gaagtggcaa accatctggg
     9481 cactgtgcat catgaaattc acttcactgt acaggaaggt ctggacgcca tccgcgacgt
     9541 gatttaccac atcgaaacct atgatgtgac cactattcgc gcttcaacac cgatgtattt
     9601 aatgtcgcgt aagatcaaag cgatgggcat taaaatggtg ctgtccggtg aaggttctga
     9661 tgaagtgttc ggcggttatc tttacttcca caaagcaccg aatgccaaag aactgcatga
     9721 agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgtg ccaacaaagc
     9781 gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaagaaat tcctcgacgt
     9841 ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaagatgg aaaaacacat
     9901 cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc agaaagagca
     9961 gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg cggcgcagca
    10021 ggtttctgat caacagctgg aaactgcccg cttccgcttc ccgtacaaca caccgacctc
    10081 aaaagaagcg tatctgtacc gggagatctt tgaagaacta ttcccgcttc cgagcgccgc
    10141 tgagtgcgtg ccgggcggtc cttccgtcgc gtgttcttcc gctaaagcga tcgaatggga
    10201 tgaagcgttc aagaaaatgg acgatccgtc tggtcgcgcg gttggtgttc accagtcggc
    10261 gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca gttattgagg
    10321 tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat cactgtttga
    10381 caactcatca ctgtaggcca gtatttcata gattaatttc ccaccactat tgttaaattc
    10441 cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg ggaaaaaaca caaagtaaac
    10501 aataattgac gaatatagcg ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc
    10561 aattttcctt gaaaaagagg ttgacgctgc aaggctctat acgcataatg cgccccgcaa
    10621 cgccgataag gtatcgcgaa aaaaaagatg gctacgtagc tcagttggtt agagcacatc
    10681 actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt ttttttgcgg
    10741 gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt
    10801 caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc
    10861 ggtaaggcac cggtttt
//
LOCUS       DAZKFZ010000157        10650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000157 DAZKFZ010000000
VERSION     DAZKFZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..193
                     /locus_tag="ACHOQW_003519"
     CDS             <1..193
                     /locus_tag="ACHOQW_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574539.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
     gene            310..1608
                     /gene="kgtP"
                     /locus_tag="ACHOQW_003520"
     CDS             310..1608
                     /gene="kgtP"
                     /locus_tag="ACHOQW_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP9574540.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHOQW_003521"
     CDS             complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHOQW_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP9574541.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHOQW_003522"
     CDS             complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHOQW_003522"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP9574542.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHOQW_003523"
     CDS             complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHOQW_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP9574543.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHOQW_003524"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHOQW_003524"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP9574544.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHOQW_003525"
     CDS             complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHOQW_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP9574545.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHOQW_003526"
     CDS             7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHOQW_003526"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9574546.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHOQW_003527"
     CDS             complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHOQW_003527"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9574547.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            9607..9990
                     /gene="grcA"
                     /locus_tag="ACHOQW_003528"
     CDS             9607..9990
                     /gene="grcA"
                     /locus_tag="ACHOQW_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP9574548.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="ACHOQW_003529"
     CDS             complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="ACHOQW_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP9574549.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
BASE COUNT         2625 a         2711 c         2748 g         2566 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgcctg atgcgacgct
       61 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggtttc cccaacttgc
      121 ctacttccat acgtgtcctc cttaccagaa atttatcctt aagctcctca ataaccattt
      181 tcctgctaac taaattcatg gttaaggttg cataatgata cgtaacaaat gtataacatt
      241 ttctttgcca aaagaaataa acaaaagcga ccgacaaaag catcggatta cggcaggaga
      301 cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgatact
      361 cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga gtggttcgat
      421 ttctatgtct actcgttctg ttcactctac tttgcccaca tcttcttccc ttccgggaac
      481 acgacgactc aactactaca aacagcaggt gtttttgctg cgggattcct gatgcgccca
      541 ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgctg
      601 ctatcggtgt gtatgatgtg tttcggctca ctggtaatcg cctgtttgcc tggttatgaa
      661 acaatcggta cgtgggctcc ggcattattg cttctcgcgc gtttatttca gggattatcc
      721 gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc
      781 aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcggacaact gctagcccta
      841 ctggttgtcg tggttttaca acacaccatg gaagactctg cactcagaga gtggggatgg
      901 cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcag
      961 ttagatgaaa cttcgcaaca agaaacgcgc gctttaaaag aagctggatc tctgaaagga
     1021 ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt
     1081 tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc gggaatgcat
     1141 gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca
     1201 ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg tttcggttcg
     1261 ctggcagcca tttttaccgt tcctattctc tcagcattgc aaaacgtttc ctcgccttat
     1321 gccgcttttg gtctggtgat gtgtgccctg ctgatagtga gtttttatac atcaatcagt
     1381 ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt tggtctgtca
     1441 tatgcggtcg ctaatgctat atttggtggt tcggcggagt acgtagcgtt gtcgctgaaa
     1501 tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcgttt
     1561 ctggtttctt tgatgctaca tcgcaaaggg aaggggatgc gtctttagtg acgggtcagt
     1621 tgccagacgg tatagccggt gcttgcaccg gcgacatccc aggccaaatc cttccagctc
     1681 cagccgctcc cttcggggcg gctatcccaa aactcttttg acgcccccaa actgacagag
     1741 aacatcaaac caaacatggc actgcgatcc cggctcatcc cctgatgctg tgaatattca
     1801 tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc
     1861 cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca gacaaaaaga
     1921 atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg tgattacagg
     1981 atgcggctaa ttaatcggtc gatgcggata cggcgcaaac ggcggatgag tttacgaacc
     2041 ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat ggtggtatgc
     2101 tcgcggatca gctccagttc tttctctcgc tgcggcgcaa gctccagttg tggatcgtgg
     2161 atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt attaccggtg
     2221 atcaacatcc acttatcatc aacccacatc ccttttaggt gataggtgtt gtcgtcatct
     2281 ttccataacc gaaccactag ctggtcagta ttgacgtaat actgcaaacg gctcaggaaa
     2341 cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa aggttcatct
     2401 tccggaatgt agaagtcgtt cgcggtttta tcaccaacaa taatttcgac ctttttccct
     2461 tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa gtatggcgta
     2521 cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt gttcaacaga
     2581 ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc ggcatcgccc
     2641 tggaaatgat aagcggcatc acgcagctcc tggcggaaca gacgaatatc gttcttgatt
     2701 tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc attcataata
     2761 ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat aagatgataa
     2821 cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct ggcaccgcta
     2881 taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag ggcttcacgg
     2941 gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg cgccatgcga
     3001 cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg ttgtgcacga
     3061 tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc ttcatacaac
     3121 gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac aatgcaaatg
     3181 cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc gggagcgtaa
     3241 aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag gtgttgttga
     3301 tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt tcattgaagg
     3361 gtcctctgtg caatgcagac gacataagcg tgcaataata acaccagtcc cgattaagtg
     3421 gtcaacattt ccagtacctt actcatgatt cctcgcgttg ggcaagattt agcgtaagcc
     3481 caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg gccagcgcca
     3541 ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg tgatctcgcg
     3601 tataggtaat caacttttcc attaagcgtc gacccaagcc taaccctttg agatccgagc
     3661 gaaccagcac ggcaaattcg gcatcgatgt tatcagggtc ggagatcgca cgcgtgacgc
     3721 cgaggatctc ttccgtttga tcaattcgtc gtaccgctac aaacgccatt tcccgatcgt
     3781 agtcgatctg cgtcatgttg gctaaatctt catgggtaaa ttcgttgatc tcgctaaagt
     3841 agcggtaata aagatcttct ttggtgaccc gcgaaatgaa ttgctgaagt tgtggctcat
     3901 cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct acccattctt
     3961 ccagctgatg cggataaggg cgcactgcca gccgactctc gttatcgcct tcaaacggcg
     4021 cgatatccag agtgacatcc agcgcggtaa attcactgcc agaagccagc aaaggatgaa
     4081 tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc accagaagct
     4141 ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg cgcacgaatc tttttacttt
     4201 tgatcccctg aataaccaga tagcgggcca ggttcatatt cagcggcggc agtgcgacga
     4261 cggcttgatc ttcaggacgc cactccacac cgccttcacc cagcatgatc aacggcccaa
     4321 aaaccggatc gtgctcaacc acaacccgca actcctgagc gccagcacgg ttagccatac
     4381 tttgcaccaa caggccgtgg acccgcgcct gcggccaggt catttttacg cgatcgaaaa
     4441 tagcgtttgc cgcttgctgg acttcattgg ctgtacgcag gtacagcatg acgccctgaa
     4501 cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga taaccaatct
     4561 gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtaggg agcgtgttca
     4621 tcccatacga ttgcaggatg ggctgaactt catgggtatc gagcgacgta gccccttcgg
     4681 caatcgcctg ttgcaacaga agatgcgctt ttgcggtatt ggaagtcaga ttgctgggca
     4741 acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata tgcataaaag
     4801 cagtgatggt tccttccggg gtacggtagg tcggcagccc ggcttcgctg aataaacgtc
     4861 gtgcctcttg cgaggagtgc tcgccgcacc agttcgtcag cagagaaaca tacttgctgc
     4921 ggggatgatg ctttaccgct tcaattaata cttgcgcgat ttctgttgcg ggagcagcgg
     4981 cgctgggcga atgaataacc atcagcgcat caaaatcctg gctgtggagc agaatgtcca
     5041 gcgttttgac ataatgctcg ctgctggcgt catcgcgcag atcgagcggg ttagagattg
     5101 ccacatgtcc tggcagtgca tcgcgcagtt tctggcaggt ttcttcgctt agcgttgcca
     5161 gcttgccatt gcgtgaccat aaggcatcca gcgccagcgc ggcaggcgca gcaccgttgc
     5221 tgataatcat caaccggtcg ccacgcagcg ggcgcatatg gctgagggtt tccaccgccg
     5281 aaaacagctc gtgggtatcc tgcacccgca acaaaccggc acgctgaatc gccgcatccc
     5341 acgccggatc cattcctgcc gtcgtgttga gcagtcgctg tgctgccggg ctacgtccgc
     5401 ttttaatcac cagaatcggt ttattacgcg aggcactacg ggccgccgaa acaaagcgtc
     5461 gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg tcgcgtgcca
     5521 gatagtcaag caattcatca acgtcgatat ctaggctgtc gccgagcgca ataaagtagg
     5581 aaaagcccat ctcacgctgt tgcgcccagt cgaggatggt gttggagacg gcagccgact
     5641 gtgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg gcattcagac
     5701 cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg ttatggcgca
     5761 gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata atgcaggttt
     5821 tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca ttggtacata
     5881 aaaccgcaag gtcgggtgta aagggcaagc tggcaatatc cggccaggcc aacacaccca
     5941 acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct cccgccagca
     6001 ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgacgcgcca attaccgcta
     6061 tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc tcccgcagaa
     6121 gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc cagatgttgc
     6181 gtttttcctg ccagatagcg cgttttaaag cgggtgaagt gctcgcctaa ccctgctgcc
     6241 gccccggtat cgccggccat atctaacagt gcgatggcta cctcggcagt acaatattgg
     6301 ccttcagcct gggcttcacg caggcgatag gcagaaagcc gggaaagatc gacggaaatg
     6361 acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc cggccaggta
     6421 ccatcgagca tgataaacag cggtggctta ccggcaggtg gtgtgaagat cacttcccgt
     6481 tgctcatcag catacgaggc gggaaagacc accattggct gataatccgg gttttgtacc
     6541 agatccagca aatcctgcga gggttcggta cgcgaccatt gaaacgcaac ggtatcaggc
     6601 aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt gtcgaacatc
     6661 agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag acataatttc
     6721 tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag aaaaggacgt
     6781 gttgcgcgcg caatacgctc ggcgcgtaac tggagaacag cgttttcggt cataagagag
     6841 cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg ccccggtaag
     6901 attaaagaga ttcgttcagc cagctatcga acggcgcttt cggtactgcg ccattaagca
     6961 tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga attccaaagc
     7021 gactgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact ttaccgctac
     7081 gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg caccacggtg
     7141 cccagaagtc gatcaccact ggtagatcat ctttcagcaa tttgtcgagc gtttcaccgg
     7201 tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat tttgccgcat
     7261 cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa acggtattca
     7321 taactaacct cgggatgagt aaggagactt atcggcaaca acgccggtat gtttctaata
     7381 tgttacatat tatcggggac attgttccag acaacaatct gttttattca ataggtatga
     7441 tagtcgcagg ctttggtacg aaatcatggc gaaccgcgcg gacttcgggt aatctgcgcg
     7501 cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa aagcggcaag
     7561 gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca caacccgcgt
     7621 accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg tcgccccgcc
     7681 cgcgatgaca aacagagtca gccccgtgac cgcaagtggg aagattcgcc gtggcgcacg
     7741 gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg catcagtggt
     7801 aaaagtttta ttgatccgga agtgttgcgt cgtcagcgtg cggaagaaac ccgcgtctac
     7861 ggcgaaaacg cctgtcaggc actgttccag agccgtccgg aagcgattgt tcgcgcctgg
     7921 tttatccaga gtgtaacgcc gcgttttaaa gaagccttgc gctggatggc agcaaaccgc
     7981 aaagcgtacc atgtggtaga tgaagcggaa ttgacaaaag cgtcaggcac ggaacatcac
     8041 ggtggcgttt gcttcctgat caaaaagcgt aatggcacaa ccgtgcagca gtgggtaagc
     8101 caggcaggcg cgcaggattg tgttctggca ctggaaaacg aatctaaccc gcataacctg
     8161 ggtggcatga tgcgcagctg cgcgcacttt ggcgtgaaag gtgttgtggt gcaggatgcg
     8221 gcactgctgg agtcgggggc ggctatccgt accgcagaag gcggcgcaga gcacgttcag
     8281 ccgattactg gcgacaacat tgttaacgtg ctggatgatt tccgtcaggc gggttacacc
     8341 gtagtgacaa cttccagcga gcagggtaaa ccgctgttca aaaccagtct gccagcgaaa
     8401 atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg cgatccgaac
     8461 gatctgcgcg tgaagattga tggtactggc aacgttgccg ggctgaatat ctctgtcgca
     8521 accggcgttc ttcttggcga atggtggcgt cagaataaag cctgataaat cagccgggtg
     8581 gcaactctgc catccggcat tttcccgcaa atttactcac tctctgccgg taatactggc
     8641 atccagtcaa tcggcgtctc gccatgttgt tccagccact gatttgccag cacaaaatgg
     8701 ttgcagccaa agaatccacg atgagccgaa agcggcgacg gatgcggtgc tttcagtaca
     8761 tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgcgatcc ccacaacaaa
     8821 aacaccacgc cttcgcgatg ctggttaatc aggctgatca ctttatcggt gaaggtttcc
     8881 cagccgaggc tggcgtggga atgcgcctga cctgcgcgta ccgtcaacac agtattgagt
     8941 agcagaacgc cctgacgcgc ccagctttca agataaccat gattagggcg ggtgaagccc
     9001 ggaatagtat tttccagctc tttatacata ttcagcagtg acggaggagt ggcgattccg
     9061 gggcgaacgg aaaatgccag cccatgcgcc tgtcccggtc cgtgataagg atcctggccg
     9121 agaatcacca ctttaacgtc acccaactcg gtaaagcgga acgcgttaaa gacatctttt
     9181 tgtggtggat agatagtgac gccggactgc cgctcgctgg cgacggtctg aagggtatta
     9241 agaaaatagg gttgctgctt ctcttcagcc agcacgtcat gccaggttaa ttcgttagcc
     9301 atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc tacagaacaa
     9361 aatcatgctc attttttaga cttaaacgag tgcaaaaaaa tgtaaattgg taaaatttca
     9421 cacccgcata agttgatgta aaacaataaa atctatttgc gaccacactt gatgtgtggt
     9481 ttttattgat ttaaatcaaa gattcaaggg tgtttgagga gtatatatac actcaagcaa
     9541 caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt gccggggagg
     9601 catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct gctgaactct
     9661 ttctggctgc tggacagcga aaaaggcgaa gcgcgttgca tcgttgcgaa agcaggttat
     9721 gcagaagacg aagtggttgc agtaagcaaa ctgggtgaca ttgagtaccg tgaagttcca
     9781 gtagaagtga aaccagaagt tcgcgttgaa ggtggtcagc acctgaacgt taacgttctg
     9841 cgtcgtgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca gctgaccatc
     9901 cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca gcgcgacgtt
     9961 atcgctcgta cctttactga aagcctgtaa ggcttaagta gcatgttact aagcggaaga
    10021 gggcgacctc ttccgctttt tttcattaat agaaaatgcg taccgcgcaa tagatcagca
    10081 gcagcgccag cacgatattt aactggcgac catactggcg aaacaatcgc tgaaacagat
    10141 gccccgccag cgcccagcac acattaccaa acgtcccaat cattgccagc aaaacgctga
    10201 cgccaactat ccagcttaac gcctgtgttt gcggcagaac aaatgtcgac agtgccgtaa
    10261 caccgtacaa aatgattttg acgttcacaa actgcaaagc aaagctggcc caaaaactga
    10321 ttggttttgt ctgaagcccg tcttcctttg ttggactggt agcgattttc cacgccagcc
    10381 agacaatata tgccgccccc gcccaactca acagatgcac agctgccggg tcgataacag
    10441 ccagtgaaaa cgaaatgccc gcacacagca acatgacgat taaaaatccc agactcatcc
    10501 ctgccagcac gcgggtactc tgatgaaatc cgtgcgttgt ggctgagcta agggcgagaa
    10561 tattgttcgg tcctggcgtc atggcggtaa tcagggtgta agtccaaaaa gcacttaaaa
    10621 gggtcggtgt cacttgttat ctcctctgtt
//
LOCUS       DAZKFZ010000158        10630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000158 DAZKFZ010000000
VERSION     DAZKFZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            126..557
                     /gene="osmC"
                     /locus_tag="ACHOQW_003530"
     CDS             126..557
                     /gene="osmC"
                     /locus_tag="ACHOQW_003530"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP9574550.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(613..1539)
                     /locus_tag="ACHOQW_003531"
     CDS             complement(613..1539)
                     /locus_tag="ACHOQW_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9574551.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(1532..2518)
                     /locus_tag="ACHOQW_003532"
     CDS             complement(1532..2518)
                     /locus_tag="ACHOQW_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9574552.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2515..3411)
                     /gene="ddpC"
                     /locus_tag="ACHOQW_003533"
     CDS             complement(2515..3411)
                     /gene="ddpC"
                     /locus_tag="ACHOQW_003533"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP9574553.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3408..4430)
                     /locus_tag="ACHOQW_003534"
     CDS             complement(3408..4430)
                     /locus_tag="ACHOQW_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9574554.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPRGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4432..5982)
                     /locus_tag="ACHOQW_003535"
     CDS             complement(4432..5982)
                     /locus_tag="ACHOQW_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9574555.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(5996..6577)
                     /gene="ddpX"
                     /locus_tag="ACHOQW_003536"
     CDS             complement(5996..6577)
                     /gene="ddpX"
                     /locus_tag="ACHOQW_003536"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP9574556.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(6835..9224)
                     /gene="dosP"
                     /locus_tag="ACHOQW_003537"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             complement(6835..9224)
                     /gene="dosP"
                     /locus_tag="ACHOQW_003537"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            complement(9249..>10630)
                     /gene="dosC"
                     /locus_tag="ACHOQW_003538"
     CDS             complement(9249..>10630)
                     /gene="dosC"
                     /locus_tag="ACHOQW_003538"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP9574557.1"
                     /translation="EMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYRI
                     VRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIGI
                     PVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDSS
                     ASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFGL
                     WFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVS
                     QIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVD
                     KFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRT
                     AERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRN
                     RVELWKASL"
BASE COUNT         2613 a         2759 c         2666 g         2592 t
ORIGIN      
        1 gtgggaattt aatttaagtg cagaagtaat atttttgccg tattttattc ggaatatcct
       61 gcttatcctc gtgcggtttc tcacgtagtc tataatttcc tttttaagcc cacaggagag
      121 caacaatgac aatccataag aaaggtcagg cacactggga aagcgatatc aaacgcggga
      181 agggaacagt atccaccgag agtggcgtgc tgaaccaaca gccgtatgga tttaacacgc
      241 gttttgaagg cgaaaaagga accaaccctg aagaactgat tggcgcagcg catgccgcat
      301 gtttctcaat ggcgctttca ttaatgctgg gggaagcggg attcacgcca acatcgattg
      361 ataccaccgc cgatgtgtcg ctggataaag tggatgccgg ttttgcgatt acgaaaatcg
      421 cactgaagag tgaagttgcg gtgccgggta ttgatgcctc tacctttgac ggcatcatcc
      481 agaaagcaaa agcaggatgc ccggtctctc aggtactgaa agcggaaatt acgctggatt
      541 accagttgaa atcgtaaagc attgccggat gacgcgtcag gcgcatgaat gcctgatgcg
      601 ttgttagcat ctctacagcg cccgccagca acggatctca cgtccgtcag cccgcgtcgt
      661 taatgattgc cggacttcgc atccgctggt tgcaaggggg caacgttcgc ggaaaaaaca
      721 gccctgtggc aacgtgcggt ttcctggcag atccgtttta cgtaatgccc attcttcctc
      781 cagtggttta tcaatggcgg ggagggaatc cagcaataat cgggtgtatg gatgtgccgg
      841 agccgttagc acctgttgcg cttcccctaa ttccacaatc tgcccgagat acatcaccgc
      901 cacccgatcg ctcatatgac gtatcaccga gacattgtgt gaaatcagca cataagtcag
      961 cccgtgattt tcctgtagcg ttaccagtaa attgaggatc tgcgcctgca cggagatatc
     1021 cagcgcagag gttggctcat caagcacaat cacgtcaggc tgcgaagaga gtgctctggc
     1081 aatggcgatg cgttgccgct gcccgccgga gaacgcatga ggcaggcggt cgagatactc
     1141 cggacgaata cccacctgca cagccagctc ctctgccaac gctcgccgct gttgttcact
     1201 actatgctta gctatccaga gcggttctgt gatgatgcgc cacaccggta agcgcggatt
     1261 aagcgaagag agcgggtcct gaaacatcat ctgcataatg cgttgtgagc cagaacggat
     1321 gtactgcccg tggctcggtt gcagcatacc cattaaaagc tgtgcgaggg tgcttttgcc
     1381 gcagcctgac tcgccgacga tccctaaggt ttcaccacgg cggatctgta aatcaatacc
     1441 attaatggca tgaacatgtt ccgtagtttt accaagccag tttttacgcg ccgggaaatt
     1501 gatatggacg tcgcgtaacg ttaataacgt gtcagacact tatgacctcc tgctgcggat
     1561 accaacaggc gcagcgctgg ttgttgtcac cacacgctgt cagcgccggg acgttttcac
     1621 actgtgcacc tgccgcatag caacgatcgc gaaaagcgca gccgtcaggc aaatgggtga
     1681 gatttggtac cgtccctgga atggcgggta atggctggcg tggtactcca tgttctggtg
     1741 cgcattgcag caaaccaatg gtatacggat gccggggatg atggataacg tctgccgtca
     1801 cgccgctctc aatcacgctt ccggcataca tcacgtaaac gctatcgcac agttgcgaca
     1861 ccacggccat atcatggctg atgaacagta ccgcagttcc actgtcgcga gctttatgtt
     1921 taagcaaacg cagtacctgc aactgtaccg tgacgtccag cgccgtagtc ggttcgtcgg
     1981 caataatcaa ttgcggttcg caggagaatg ccagcgcaat cattacccgc tggcgcatac
     2041 cacctgaaag ctcaaacgga tagcgcgaca taacttccac ggtatccggg atttgcatct
     2101 cttccagcag ggcaatcgct ttagctctgg cttcccgacg acttattggt tgatgatggc
     2161 ggatcacgtc catcatctga agacctattc gacgtgtcgg attgagggcg gtcatcggtt
     2221 cctgaaagat catcgccact cgtgcgccgc gccactgacg aagctgtttt tcccgcgcgt
     2281 taagaacatc gtctcccagc agtgaaatct gcccccgatg tacgcaataa ctgcccgtcg
     2341 gtagcagacg cataatcagc attgcggtga ctgatttacc tgagccggat tctcccacca
     2401 gaccgacaat ttcaccgcgg ttaatctgca aggacacatt gttgagcgcg tgaacgtcgc
     2461 cgttaaaacc ggggaaactc aaatgcagtt gttgaatgtc cagaacgggt tgggtcatga
     2521 ctgctttcct cctgctttcg ggtcaagcag atcgcgaata ccatcaccaa agagattaaa
     2581 cccgacggcg gtaagcaaaa tcgctgcccc cggaaatgcg caataccacc actgatcgag
     2641 tacatagttg cgaccatttg ctaccatcgc gccccattca gcactcggtt gttgagcacc
     2701 caggccaata aatcccaacg tggcggccat taaaatcgcg ctaccgatat ccagcgatgc
     2761 ctgcacgatc agcggcggta gggagttacg taaaatatgc cagttgatca gatgccaacg
     2821 agacgcgcca aaggttttcg ccgcctgaac ataggtatac tggcgtacaa ctaatgcttg
     2881 cccccgcgcc aggcgcacat aaaagggaat tcgcacaata gcaatagcca gcatggcgtt
     2941 aaacaaactc ggcccgagag cggcagccag tgccattgtc agtaccaacg aaggaatcga
     3001 cagcataatg tccatgatgc gcatgatgat ggcgtctgcg cgtccgccaa gcacaccgga
     3061 tagacatccg agtagcgaac caatcatacc cgcaatggca accacgacta atccggcgag
     3121 aattgattgc tgactgccga ccagtacgcg gctgaacaga tcgcgtccca cttcatcggt
     3181 gccaaaccag tgcgccgcag aaggcggcaa aaggcgggcg gttaaatcaa tggcgttggg
     3241 atcatgcgtc gccagccacg gtgaaaaaat catcatcagt agcattaata caatgatgac
     3301 tgcgccggtc acggtgagcg gactgccttt cagcatccat accagttttg caccgttaaa
     3361 tcgcgtttgt ttttgtgggc gtacggcgga cgtttcctcg cttagcatca ttcaccacct
     3421 ccacgtccga tacgcggatc aatccacaaa tagagcaaat ccaccaccag gttgaccagc
     3481 acataagcaa atgaaaccac gacggcaaag cccatcactg ccgggaagtc gagcgcctgt
     3541 attgatgtca ctacccatgc ccccattccg ggccaggcaa aaacggtttc ggtgagcact
     3601 gcgccataca acaaatcgcc cagcgccagc cccaatacgg taatcgaagg gatcaacgca
     3661 ttgggtagcg cataacatag aacgatatac cagccgggca agccgctggc ccgggcggta
     3721 cgaatgtagt cttcactcaa ttgttccagc atcgctgagc ggatctggcg ggcgacaatc
     3781 cccaggtgaa caaacgccag cgttaatgcc ggtaagatga gatgttgcaa cgcattgaag
     3841 aagacttcac cgttgccttc aagcaacgca tcgagcagat aaaagccggt aacgtgcgtt
     3901 ggtggatcca gccagtcatc aagccttccg ccgccgggaa gtatttgcag atgaccataa
     3961 aacagcacga tgacgcccag cccaagccag aacgcaggtg tggagattcc ggtaatcgcc
     4021 attattcgca ccagatgatc cagccagcga tttcgccaga ctgcagagag tatgccccgc
     4081 gggatgccaa tcaagagtgc cagcagcagg gcacaaaaag ccagctccag cgtcgccggg
     4141 aaaaatatac gcaactcttc cagcaccgga cgcccagtac gaatggatgt tcccaggtca
     4201 ccatgaaaca ggtcgctgac gtagcggtaa aactgtacat acagtggctg gtccagtccc
     4261 agttgctggc gaatattttc cacgatagca tcgctggcgc ggtcacccgc cagtaaccgc
     4321 gccggatcgc cggggatcag atgcgagata ataaacgtaa tcacgcagac gcccgccacc
     4381 acgagcacca gcccccagca gcgttggcgt aaaatactcc agaaggtcac gttatttact
     4441 catggtattg atattgaaga cctgttccag catgggattg aacacaaagc ctttcacctc
     4501 tttgttcatc gccagttggt agtttttctg gaacaggtac acataagcag cgtcatcaat
     4561 gacgattttc tgtgcctgct ggtagtctcg ggtacgctgc gtctggtcgg tggtcgcaag
     4621 cgcattgcgc agtaacttat cgacctcact gttttcatag aacgagcggt tacccggcag
     4681 accttttttg tctgactcaa accagtaatt cataaacata tacgggtcgg caaaatccgg
     4741 actccagttg ccaatcgcaa tgtcgtaatc acctttaccc actctgtcgc gcatggtggc
     4801 gttcgccagc ttttccagct tcacattgat gcccagcttg ttgaggctgg attgtgttgc
     4861 cagagcgatc ggttcccagt tcggatcgtt atcggagtag agaaacgtca ggctggtggg
     4921 tttgctcgtc actttatccc attcggcttt ggctttcgtt tcgtcatggt tgtattgcat
     4981 tgccgtcgca tcgtagcccc acatgccttc cggaatcggg ccgcgcattt gttttccgtt
     5041 accactcaga atgccgttaa ccatgccctg ataatcggta gaccaggaaa tggcccgccg
     5101 cagatccgcc tgattaagtg gcgctttgct gttattcaga tacagatagg tgacgcgcag
     5161 tgacggatac tctgccacat tgactttgtt ttcctgcttc agggcgttga gttgatccac
     5221 cggcagcgca tcggcaatgt caatgtcgcc acgggagagc tgcaggcgac gggaggcact
     5281 ttcaccgata atttttaccg atactcgctt aaagttcggt ttattgccgg ggtaatgcgg
     5341 atttggcacc agaactaatt gctgaccttt ttgccagctt ttcagcataa atggtccgga
     5401 accggcggta ttttgcgcga ggaagccgcg ggcatcatcc gccgcatgtt cctttaatac
     5461 cgccggattg ataatggatg caccgtcatt cgccagcgtg tagaggaacg gtgcaaatgg
     5521 ctggctaagg gtaaacttca ccgtatgttc gtcgggagca tcaatcttta aatctttggg
     5581 aaatgcttct gctggcccct ggccgatttt tagtaaccgc tcaaaagaga gttttactgc
     5641 ttcggcagtg acaggtgtgc catcggcaaa tttagcatta tctttcaggg tgaacgtcca
     5701 ctctttttga tcgttagacg ctttccagct acttgccaga tcgccttcaa cgtcggttga
     5761 gcctttatca ccgtccgttt tgtactgaac cagtcgctga taagacgggt aggtcactgt
     5821 ccagtcgtta ttatctattg ttaccgccgg gtcgagggtt tgtggatcgg cggccttacc
     5881 aatcaccagc atatctttcg gtacggcggc gtgcgcaacc gggaaagttg tggcaaggac
     5941 gagcgcgagc aatgtgggac gaaacgatat cgatctcttc atggcagttt cctgattaac
     6001 tgacgtgctg tgtgccaggg gatataaaac aggtgaattg atcagcgagc agagggtaac
     6061 tcgctgcctg gggtaattcg aagtgccacc attcgctgga gataccgaca aaaccgccac
     6121 cagtcattat cgcattcagc aacaggcgat tgcgctgagc ggcgggcggg acggaagggt
     6181 gataggcatg ggagcgctcg tgcatttcat cgaaccctgc gcccatatcc aggatgttcc
     6241 cgtgctcatc acgaagcgtc aggtcgatcg ccgtgccacg gctgtgatta gaaccgaccg
     6301 tcacatcaac aacatattgc gggtctgggc aggcttgcca caacatcgcc tgtgcttgtt
     6361 gtgggcgata cgcatcgtaa atcaccagtt gtaagcctga tagctgggcg atactgatac
     6421 ttttcgccag cgcggtaatc gcatccttgt gtaacagaca acgcgcttgc tggtaaatag
     6481 ctttacctgt gatgttatca gcgcaggcgt atttcaattc gatctccaga tcagggaaga
     6541 tcacggctaa atcaaccagt tcggtggtat ccgacataaa cggttcctgt ataagacaaa
     6601 aattgctgcg ctttccgctt atgcagatct catgccatgc cgggataagc gccagaatgc
     6661 tggcttaaag ttatattttt taacttttga tcaacatttg tgcagcgtag tgcagttttg
     6721 gtgcaagagg ggaagttaag gaaggaatct cccggaatcg tagctgaaat cacagtattt
     6781 aagtgacagt gtcacgttaa atgaaaaccc gcgagtgcgg gagagaggaa tttgtcagat
     6841 tttcagcggt aacacgctgc tcatccagcc tggaatttct tcggcgggta gggggcggga
     6901 aaagaaatat ccctgaataa cgcgacagtg gatctttcgt agcatctcaa attgctcttt
     6961 ggtttcgacg ccttccgcca cgacggttaa attgaggctt tgcccaatgc tggtaatggc
     7021 ttcaagtaag gccaggatgc gtttttccgt cagacaacga tcgacaaaac ttttgtcaat
     7081 tttgatttcc gttaccggaa gactgactaa gcgggataat ccggaaaagc ccgtgccaaa
     7141 atcatctacc gataaaccca cgcccatatc acgcaggatc tgaatgcgct taaagatttc
     7201 ggtatcgtgt tccatcatca tgctttccgt gatttctacc gtcagctggt ggccgtcaat
     7261 accccaggct tgcattgcat cagacacctg attaggcagc tgattactgc gaaagtgcag
     7321 cgccgacaag ttcacggata acgccgggat atgaatattc tggctacgcc attctgctaa
     7381 ctgacggcaa gcttccgcga tgacccagcg cccaatattt tcgatttcac caatctcttc
     7441 tgcgagagga ataaaccgtg aagggggcac atgaccatgc tggggatcgt gccagcgagc
     7501 aagggcttcg atgccgtaga gttcacccgt ttctgcgaag atttgcggct ggtaaaccag
     7561 tttcagttgg ttattgctaa tcgcttcttt cagcgctgcg cctaaaacca aacgctcttt
     7621 taccatttcg ttcatcgccg ggctgaagaa ctgccagccg ttaccgccat tcttgcgaat
     7681 ataatccatc gcgttatggg cagtggagag caggtaatcg cgatttttac ccacgtcgta
     7741 gctgatgcca atactcaagg taagcgggaa ggggttatcg tcaatcatta tcggcttgct
     7801 gaccacattc cgtagctcat cggcgatctg ggtaatgtta ctgacgtcgt tttccaggct
     7861 cacgaggaca aactgcgtac cttcgatacg acagagatac tgatccggtt tgagtttttc
     7921 acgaaagcga ttgaccactt ccagcaatgc ctgatcggcc cacgcatagc caaggctatc
     7981 aatcacatcc tgaatatggt caacaccgat actggagaga cggctttgtc gaccaggtca
     8041 tcgaggtaat tgtgcaggtt attgcgattt ggcagaccgg tcatcggatc aaattggatg
     8101 agttgttcaa tatgctgacg gcttttttcc tgttccagcg ccagcgcggc catatgctgg
     8161 ctgatatctg ccacgcgttc gataaaggcg ctggtttctg ctcctgacga ggttttaatt
     8221 tgcaggatcc ccgcaggcgc gccatcacgc tgacgaatgg tcgctgacca gctttgcgca
     8281 ttttgaactt ctgcaccgtg cgaagatgac gcccagtgta tcggcatccc gttgcgcagt
     8341 gcgaacagcg aaacatgcga ttcgttgaga acagattcga tgttacgaca aatgatttcc
     8401 cccatttcat gaaatggcgg gctgctgcac atggcggcga gaatattgcc ttcaagctgg
     8461 cggatttgcc gttcttcggt gatatccgag aaggtcatta ccaggttctg cagatgcgcg
     8521 agcacgtcat aaaccgggct gatagaagct ttaatccaga ttttttcacc ggtgcgcgtc
     8581 aacagcagaa attcgtcctg atcgcgggcg gttttccaaa gcaactgttg taaacgaatg
     8641 cggttatcgg cagggaattc aggaatgttc aggagtgtat caggctgcat accgctggct
     8701 tcgctaatgc agtaaccaaa catttcggta aatgcgcgat tgcactgcac aatatggcgt
     8761 tccggatcga ggacaatcac cggtcggtcg agatggtcaa cggcaataat caattgcctg
     8821 gtctgttctt tttgcgccat ttctacgctg gcatcccgta ccagcgccag gtaataaact
     8881 ttcccctcgg cgctcacttt cgatagcgca aaacgggtcc agattttact gccgtctttt
     8941 ttctccagct gcagctcccg actcatcccc tcgacgcgcg ctttaccgcc ttcacggttg
     9001 tgacgaatgt attcaggatg cgcaggacgc aaatcccgcg gaatcagcat atcaatgtta
     9061 ttgccaatga cttcttcacg tttgtacccc cagagcttct ctgcggcggg gttgaaaaac
     9121 atcacttcat cattttcgtt aattaacacc gcacccatca tattttgctc aagggcgggg
     9181 aaaaaaatgc catcggcggc attatccgca tcggttagct tcatgattac ctctgcatcc
     9241 tggcgcatct aaagactggc tttccagagt tcaacacggt ttctacctcg tcttttggcg
     9301 atatacagag cttcatcggc tatttgaatg aggcgctcat agtcaggatg accattaaac
     9361 atggcggcac cgatggaaag tgagagggca atatcttcgc cgtttgcggc tttcagtttg
     9421 gttttctcca cccgactgcg aatacgttct gcggtacgta acgtttcgtt ttcagaagct
     9481 tcagtcaaaa caatgataaa ttcatcgccc ccgtagcgga aaacataatc actactgcgg
     9541 acgttgtcat aaaaggcctg agagacttta cgcagaattt catcgccagt gttatggccc
     9601 cacgtatcgt tgatctcttt gaatttatca acgtcaataa tcagcactga cagcggtgta
     9661 ccggtccggt tggcatgggc aatttcgcgt ttgaagatag tcggcaggaa acggcggtta
     9721 agtaatttcg tcagtacatc cataccgact tcgtggcgcg atacttcttc aaacaattca
     9781 cgcagcaagg taataatttg cgatacggta tttcttatct gtaataaaaa tttcacccgc
     9841 agacttctgt tattcaaatt cctggtgtta cgcatggttt gattgaaaat accgtcgaaa
     9901 tcctgaatca gacgagagat atggcccact tcagcaatgc cgctgaaata atgtcgacct
     9961 ttatggttaa accacaggcc aaaatcagcc tggcttaaag gcaaactact gcctaaatca
    10021 gaatccagca ggattttata gataatatct atttcccatg aaagtattga ggctatttgc
    10081 cgttcttttt cttcttcggc gttttccagt aacgagaaga tacgatagtt ttcatcttct
    10141 tttgaggcac tactgtcact aaaggtaaac gcgcgggtca tcacttccat agcgatatca
    10201 atactgttaa tcgagaaatg gtagacctga agtttttctg cggcagaata atccgaagag
    10261 aagattaccg gatagaggat ctttttcagc acccgaaacc ccatctcgac aatttctacc
    10321 ggaattccta tgcgggcatg cacttccgcg acggtatgct ggatttgtat tagcctttcg
    10381 acatcgtcaa cctgggcaga aagcacgtta ataatccagc gttccatcgc acttttcaac
    10441 tgccgctcaa cttgttcatt actcaagaat tcttcggcat gcgggtcgat gcggacaatt
    10501 cgataaaact cgatactcag ataatgagca tgcgcaacgg caatttccgc ggctttagca
    10561 cgaatgggcg gatctgcctg ctcgacaagt ccggtccact catctttcat tcttttaaaa
    10621 tacatctcca
//
LOCUS       DAZKFZ010000159        10558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000159 DAZKFZ010000000
VERSION     DAZKFZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            131..1487
                     /gene="dcuC"
                     /locus_tag="ACHOQW_003539"
                     /pseudo
     CDS             131..1487
                     /gene="dcuC"
                     /locus_tag="ACHOQW_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(1530..2027)
                     /gene="sspB"
                     /locus_tag="ACHOQW_003540"
     CDS             complement(1530..2027)
                     /gene="sspB"
                     /locus_tag="ACHOQW_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP9574558.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2033..2671)
                     /gene="sspA"
                     /locus_tag="ACHOQW_003541"
     CDS             complement(2033..2671)
                     /gene="sspA"
                     /locus_tag="ACHOQW_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP9574559.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3066..3458)
                     /gene="rpsI"
                     /locus_tag="ACHOQW_003542"
     CDS             complement(3066..3458)
                     /gene="rpsI"
                     /locus_tag="ACHOQW_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9574560.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3474..3902)
                     /gene="rplM"
                     /locus_tag="ACHOQW_003543"
     CDS             complement(3474..3902)
                     /gene="rplM"
                     /locus_tag="ACHOQW_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9574561.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4121..5248)
                     /gene="zapE"
                     /locus_tag="ACHOQW_003544"
     CDS             complement(4121..5248)
                     /gene="zapE"
                     /locus_tag="ACHOQW_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP9574562.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5442..5840
                     /gene="zapG"
                     /locus_tag="ACHOQW_003545"
     CDS             5442..5840
                     /gene="zapG"
                     /locus_tag="ACHOQW_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP9574563.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5994..7361
                     /gene="degQ"
                     /locus_tag="ACHOQW_003546"
     CDS             5994..7361
                     /gene="degQ"
                     /locus_tag="ACHOQW_003546"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP9574564.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7451..8518
                     /gene="degS"
                     /locus_tag="ACHOQW_003547"
     CDS             7451..8518
                     /gene="degS"
                     /locus_tag="ACHOQW_003547"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP9574565.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8582..9520)
                     /gene="mdh"
                     /locus_tag="ACHOQW_003548"
     CDS             complement(8582..9520)
                     /gene="mdh"
                     /locus_tag="ACHOQW_003548"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9574566.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9955..10425
                     /gene="argR"
                     /locus_tag="ACHOQW_003549"
     CDS             9955..10425
                     /gene="argR"
                     /locus_tag="ACHOQW_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP9574567.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2604 a         2689 c         2628 g         2637 t
ORIGIN      
        1 aaaaatcacc tcacctacaa ttaaaaacac gacatccgca ccataaatag acttgcaaaa
       61 aatataacat cattgttttc aatctgccgt ttatgggatt gacccttttc ttttgacacg
      121 gagttcaaca atgttcggca taattatatc tgtcatcgta ttaattacga tgggctattt
      181 gatcctgaaa aactacaaac ctcaggtggt gctggctgca ggtatcttcc tgatgatgtg
      241 cggtgtctgg ttagggttag gtggtgtact cgatcccgcc aaaagcagcg gctacttgat
      301 cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac cgcattgccg gattggggct
      361 gtcgattatg gcggtgggcg gttatgcccg ctacatggag cgcacaggag ccagtcgcgc
      421 gatggtgagc ttgttaagcc gcccgttaaa actcattcgc tcgccgtata ttattctgtc
      481 ggcaacttac gtcatcggcc aaatcatggc gcagtttatt accagcgcct ccggcctggg
      541 tatgttgctg atggtcacct tatttccgac actggtgagt ctgggagtaa gccgtctctc
      601 tgcggtggcg gttatcgcaa ccacaatgtc cattgagtgg gggattctgg aaacgaactc
      661 catttttgca gcccaggtag cgggaatgaa aattgccact tacttcttcc actaccagct
      721 tccggtcgcc tcttgcgtca ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc
      781 ttttgacaaa aaagataaaa atatcaatca cgaacaggca gagcaaaaag ctctcgataa
      841 tgtcccgccg ctctattacg ccattttacc tgtgatgccg ttaatcctga tgctcggctc
      901 gctattcctt gcccacatcg ggctgatgca gtcagaactg catctggtgg tggtgatgtt
      961 actgagtttg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
     1021 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gccaacgtgg taacgctggt
     1081 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgtcg atgcggttat
     1141 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctggt
     1201 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1261 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1321 gcattttgcc acgacgctag cgcgcggttt cgccgattac tgcggtggtg gtcgttacgt
     1381 caggaattgc aggcgtttcg ccttttgcgg tggtgaagcg gacagcgatc cccatggcag
     1441 tcggtttcgt ggtgaatatg attgccacaa tcacgctatt ttattaagtc attaaaagac
     1501 aaaacaggcc gccagggcct gtttttgtat tacttcacaa cgcgtaatgc cggtcgacca
     1561 ccgcgtggtg gctgcggtgg ttcatcgtca ggatgagtgt catcatcgtg atctggcttg
     1621 tcgccatcaa taaccgacat aacggtttcg ttgtctgccg atgcctcttc atcattcatg
     1681 atgctggtat cttcatcgta ggcagcttca ggctcaaaca tcgtgcctgc gccattttca
     1741 cgggcgtaga tagccagcac ggcagccagc ggcacagaaa cctgacgcgg aatgccacca
     1801 aagcgcgcgt taaagcgcac ctcatcattc gccagttcca gattgccgac agcacgcggc
     1861 gcaatgttga gtacgatttg cccgtcacgc gcatattcca taggaacctg cacgccaggg
     1921 agcgtcacat ccaccaccag gtgcggcgtg agctggttat ccagcaacca ctcatagaat
     1981 gcacgcagca gatagggacg acgtggtgtt agctgtgaca aatccataca gattaactcc
     2041 ggcccagacg catttcacgt tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa
     2101 agacgcgggt catatagcct ttcagctctt tcgcacccgg gccgctgaac tcgataccca
     2161 gttgcggcag acgccacagc agcggagcaa ggtagcaatc gaccaggctg aactcatcgc
     2221 tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt
     2281 gcttacgtgc ggcatctgct tcagaagctg aaccgttgat gatggtgttc atcagcgtgt
     2341 accagtcttt ttcgatgcga tgcatgtaca aacggctttc accgcgagct accgggtaaa
     2401 caggcatcag tggcggatgc gggaaacgct catccagata ttccataatg atgcgagatt
     2461 cccacagggt cagctcacga tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa
     2521 tcagatcctg aggcggattg tccttttcca cgtgttcgat ctcgaaactt acacctttct
     2581 cagccagcac aatgcggacc tgatggctat agatgtcagt aggaccggaa aacagcgtca
     2641 ttaccgaacg tttgttggca gcgacagcca tgaaaacctc caggtatagt cagaattttt
     2701 actgctacca gccaccaggt ggccagtcag aagttgtgtt acccagtaag gaacgactct
     2761 ctttgttcga aaatcaaaca aaaaatgagc aatacccgac atttgggcag aaaattggat
     2821 gatagtttac cagattttgc gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga
     2881 gatgcgcttt agtctgaaat agttgactta gtcccttatt ggcgatgtgg tttttgtttt
     2941 gcctgtctgt cagacggcag caaaaagcaa ctttccattt tttacgctga ttcagaattt
     3001 aaccataaaa aaacccgccg aagcgggttt tttcgcaaat tgttttctgc cggagcagaa
     3061 gccaattaac gtttggagaa ctgcggacga cgacgtgctt tacgcagacc gactttctta
     3121 cgttcaacct gacgagcgtc acgagtaacg aagccagctt tacgcagttc agaacgcagg
     3181 gactcgtcgt attccatcag agcgcgggtg ataccgtgac ggatcgcacc agcctgacca
     3241 gagataccac cacctttaac ggtgatgtac aggtccagtt tctcaaccat atcgaccagt
     3301 tccagcggct gacgaactac catgcgagca gtttcacgac cgaagtactg ttccagagaa
     3361 cgttggttga ttacgatttt accgttgccc ggtttgatga aaacgcgagc tgcggaactt
     3421 ttgcggcgac cagtgccgta gtattgattt tcagccattg cctataatcc cgattagatg
     3481 tcaagaactt gcggttgctg tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt
     3541 ttacggaaca tagcacgacc cagcgggcct tttggcaaca tgcctttaac cgcgatttca
     3601 atcacacgct caggacggcg agcaatcatc tcttcaaagg tcgcttgttt gataccaccg
     3661 atgtggccgg tgtggtgata gtacactttg tcagtacgct tgttgccggt tacagcaact
     3721 ttgtcagcgt tcagaacgat gatgtaatca ccggtatcta cgtgcggagt gtattccgct
     3781 ttgtgcttac cgcgcaggcg acgagccagt tcagtagcca gacggcccag agttttaccg
     3841 gtcgcgtcaa caacatacca gtcgcgtttt acggtttctg gtttagctgt aaaagttttc
     3901 attaaaagct tacccaataa atagttacac gttggtgaac acccaaacgt cttcaattgt
     3961 tgaggttcac acgacaaagt ccggcaaacc taccccttcg aatagcctat gccagcacac
     4021 aaaaagtttt gggaaaaaaa ctttcttgta acgtggggtc gcaggattat agagaagtcg
     4081 gggtcaaaga tcgacccctt tttgtgattt gtggcaggtt ttaacccgct aaatgctcgc
     4141 gcttcagata ctcttcgctt tgcatctctt gcagacgtga caggcaacgc tggaactcaa
     4201 acttcagccg atcgccctga taaatttcat acagcggcac ttctgcactc accactaatt
     4261 tgacatggcg ctcgtaaaac tcatccacca gcgcaataaa gcgccgcgct tcgctctcca
     4321 tcaaccgcgt cataactggt acatcaaaca acatgaccgt atgaaagaga cgtgagagcg
     4381 caatatagtc atgctgactg cgggcgtcga cgcacagcgt agtaaaagag accgccagcg
     4441 tctggttctc gacgcccatt gtcgctaatg gccgatggtt gatttctaac gtcggtgaat
     4501 tttctcgttt cccccccgcc agcgccaacc atagtttatc catttgcgcc cgggtttcat
     4561 cgtggagtgg cgaaagccac agatgcgcct gagtgagtgt acgcagacga taatcaacac
     4621 cagcgtccac gttcattaca tcacaatgct gtttaatggc atcgattgca ggcagaaaac
     4681 gcgcacgttg caagccattt cgataaagtt cgtccggcgg aatatttgac gtcgctacca
     4741 gggtaatacc gcgggcgaac agggctttca tcaggccgcc aagtagcatg gcatcggtaa
     4801 tatcagagac aaaaaattcg tcaaaacaga gcacgtcagt ttctgcttta aaacgatcag
     4861 caataatttc cagcggatcg gtctgcccct gtaaggcagt tagctcttca tgcacccgca
     4921 gcataaaacg gtgaaagtgc aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa
     4981 ggtccatcag ccaggttttc ccgcgtccta caccgcccca catatataag ccacgcactg
     5041 gcgtatgctt tgtgtcttcc cgtttacccc acagcttacc gacccgcgcc attagcccac
     5101 tcgtcctggg ggctggtggc gtgctattga tgagttcctg ataaataatt tccaagcggc
     5161 tgacggcctc tttttgaacg tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt
     5221 attgcgatgt tggagtaacg ctttgcatga tcttattgtt attccttgaa taatcggtgc
     5281 gccgttgttc acggttgacg aaaaaaaggc cgttctacac tacgcgatat gcagtcggga
     5341 ttccacttct gtggaattaa cggttatagt ggcataatca gccgcaggca tggagcctga
     5401 agccaacacc ctacggaaac aaaagacaac gggagatgtt catgacctgg gaatatgcgc
     5461 taattgggtt agtcgtcggc atcattattg gtgctgtggc catgcgtttt ggtaatcgta
     5521 aactacgcca gcaacaggcg ttgcagtacg aactggaaaa gaataaagct gaactggacg
     5581 agtatcgcga agagctggtt agccactttg cccgcagcgc ggaattactg gataccatgg
     5641 cgcacgacta tcgccagctg tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg
     5701 aactgtctgc tgaagcaaac ccgttccgta atcgtctggc agagtctgaa gccagcaacg
     5761 atcaggcacc ggtgcagatg cctcgcgact attctgaagg cgcatccggc ctgctgcgta
     5821 ctggcgcgaa gcgcgactaa tttatttttc gggcgcagcc attgcgccct cctcttctct
     5881 ccctccccga ctatcattta atctggtgtc tcattgttag ccgtctgaaa attcaataac
     5941 atcaaactgt tttgaatctc ttttcttatc attcaggtac gagagcagga ataatgaaaa
     6001 aacaaaccca gctgttgagt gcattagcgt taagtgtcgg gttaactctc tcggcgtcat
     6061 ttcaggccgt tgcgtcgatc ccaggccagg ttgccgatca ggcccccctc cccagtctgg
     6121 ctccaatgct ggaaaaagtg cttccggcag tggtgagcgt acgggtggaa ggaacggcca
     6181 gtcagggaca gaaaatcccg gaagaattca aaaagttttt tggtgatgat ttaccggatc
     6241 aacctgcaca acccttcgaa ggtttaggct ccggtgtcat catcaacgcc agtaaaggct
     6301 atgtgctgac caacaaccat gtgattaatc aggcacagaa aatcagtatt cagctcaatg
     6361 atgggcgcga gtttgatgca aaactgattg gtagcgatga ccagagcgat atcgccctgt
     6421 tgcaaattca aaacccgagc aaattaacgc aaatcgctat tgccgactcc gataaattgc
     6481 gcgtcggtga ttttgccgtg gcggtcggta acccatttgg ccttgggcaa accgccacct
     6541 ctggcattgt ttccgcatta ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta
     6601 tccagacaga tgcttccatt aaccgcggta actccggcgg tgcactgtta aaccttaacg
     6661 gtgagttaat tggcatcaac actgcaatcc ttgcgcctgg cggcgggagc gtcggaattg
     6721 gatttgccat ccccagtaat atggcgcgaa cactggcgca gcagcttatc gactttggtg
     6781 aaatcaaacg cggtttgtta ggcatcaaag gcaccgagat gagtgccgat atcgccaaag
     6841 ccttcaacct tgacgtgcag cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct
     6901 cggcgaaagc gggcgtcaaa acgggcgata ttattaccag cctcaacggc aaaccgctga
     6961 atagctttgc tgagttgcgc tctcgtatcg cgaccaccga gccgggcacg aaagtgaagc
     7021 ttggcctgct gcgtaacggc aaaccactgg aagtagaagt gacgctcgat accagcacct
     7081 cttcgtcggc cagcgctgaa atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg
     7141 gtcagctaaa agatggcggc aaaggtatta aaatcgatga agttgtcaaa ggaagcccag
     7201 ctgctcaggc tggcttgcaa aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga
     7261 actcgattgc tgaaatgcgt aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa
     7321 ttgtacgcgg caatgaaagc atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca
     7381 ggctgacgtg tgatgtccgg ttaactcgtg gtatgctgct gccgttccct tttttaatga
     7441 cgcctccatc atgtttgtga agctcttacg ttccgttgcg attggattaa ttgtcggcgc
     7501 tattctgctg gttgccatgc cttcgctgcg cagccttaac ccgctttcca ctccgcaatt
     7561 tgacagtacc gatgagacgc ctgccagtta taatctggcg gttcgccgcg ccgcgccagc
     7621 ggtggttaac gtttacaacc gtggtttgaa caccaactct cacaaccagc ttgagatccg
     7681 caccctggga tccggtgtaa tcatggatca acgcggttat atcatcacca ataaacacgt
     7741 catcaacgac gccgatcaga tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt
     7801 gctggtggga tctgactctc taaccgatct ggcggtactt aaaattaatg ccactggcgg
     7861 tttacctacc attccaatta atgcacgtcg cgtaccgcac attggcgacg tggtactggc
     7921 gatcggtaac ccgtacaacc tcgggcagac cattacccag gggattatta gtgctacggg
     7981 tcgaatcggt ctgaacccga ccgggcggca aaacttccta caaaccgatg cttccattaa
     8041 ccacggtaac tctggcggcg cgctggtgaa ctcgctgggc gaactgatgg gcattaacac
     8101 gctgtcgttt gataagagta acgatggcga aacgccggaa ggtatcggct ttgcgattcc
     8161 tttccagtta gcaaccaaaa ttatggataa gctgatccgc gatggtcgcg tgatccgcgg
     8221 ctacattggt atcggcggac gcgagatcgc accactgcac gcgcagggcg gtggtataga
     8281 tcaactgcaa gggatcgtgg ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg
     8341 tattcaggtc aacgatctga ttatttcggt ggataacaaa ccggccatct ctgctctgga
     8401 gacgatggat caggtggcag aaattcgccc tggttcggtg atcccggttg tagtgatgcg
     8461 tgatgataag cagttaacgc tgcaggtcac cattcaggaa tatccggcaa ccaattaagg
     8521 ccagcgtgaa agacaaaaaa accggagtct gtgctccggt tttttattat ccgctaatca
     8581 attacttatt aacgaactct tcgcccaggg cgatatcttt cttgagcgta tccagcatac
     8641 cttccagcgc gttctgttca aatgcgctca gggtaccgat agatttacgc tcttccacgc
     8701 cgtttttacc cagcagaagc ggctgagaga agaaacgggc gtactgaccg tcgccttcaa
     8761 cgtaggcaca ttcgacaacg ccttgttcgc cctgcagtgc gcgaaccaga gacaggccaa
     8821 aacgtgcagc agcctggccc atagacaggg ttgccgaccc gccaccggct ttcgcttcaa
     8881 ccacctcagt acccgcgttc tggatacgtt tggtcagatc agccacttcc tgctcggtaa
     8941 aactaacgcc aggaacctgt gacaacagcg gcagaatggt aacaccagag tgaccgccaa
     9001 taaccggcac ttcaacttcg cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg
     9061 aacgaatgat atccagcgtg gtaacgccga acagtttgtt tttgtcataa acaccggctt
     9121 ttttcagcac ttcagcagca atcgcaactg tggtgttaac cgggttagtg ataataccaa
     9181 tgcacgcttt cgggcaggtt ttcgcaactt gctgtaccag gtttttcacg atgccggcgt
     9241 taacattaaa caggtcggaa cgatccatac ccggtttacg cgctacacct gcagagataa
     9301 gaacgacatc tgcgccttcc agcgccggag tcgcatcttc accagaaaaa cctttgattt
     9361 tcacagcagt agggatatgg ctcagatcga cggccacacc gggagtcact ggagcgatat
     9421 catacagaga gagttctgaa cctgaaggca gttgggtttt taacagtagt gcaagcgcct
     9481 ggccaatacc gccagcagcg ccgaggactg cgactttcat cctaaactcc ttattatatt
     9541 gataaactaa gatatgttgc tccgctgccg cgaccttaat ccacaaaatt gccgtttaca
     9601 atgaccacat ctcaagaatg tgtagtcacg caagtttagc gtttatgcat ttaattgccg
     9661 taatcaggaa cctaagttac gtaattaaca atcgcttatg cattagcgca acattccaac
     9721 aggtggtgac aatataccct accgttcagc caaaacaaca tcaatttgat aacaattaat
     9781 ttacttttaa gcagaatttg catgccgtga cgcaggcata tttctcaata acgaaatttg
     9841 ataaaatccc gctctttcat aacattattt cagccttctt cagggctgac tgtttgcata
     9901 aaaattcatc tgtatgcaca ataatgttgt atcaaccacc atatcgggtg acttatgcga
     9961 agctcggcta agcaagaaga actagttaaa gcatttaaag cattacttaa agaagagaaa
    10021 tttagctccc agggcgaaat cgtcgccgcg ttgcaggagc aaggctttga caatattaat
    10081 cagtctaaag tctcgcggat gttgaccaag tttggtgctg tacgtacacg caatgccaaa
    10141 atggaaatgg tttactgcct gccagctgaa ctgggtgtac caaccacctc cagtccattg
    10201 aaaaatctgg tgctggatat cgactacaac gatgcagttg tcgtgattca taccagccct
    10261 ggcgcggcgc agttaattgc tcgcctgctg gactcactgg gcaaagcaga aggtattctg
    10321 ggcaccatcg ctggcgatga caccatcttt accacccctg ctaacggttt caccgtcaaa
    10381 gacctgtatg aagcgatttt agagctgttc gaccaggagc tttaatctct gccccgtcgc
    10441 ttctggcggc ggggaaaatg ttgcttatcc ctctcaaccc cctgctttcc cctgcgatta
    10501 atttaacgaa tagtgcgttt tactgcgaca tgtcattcac acaatgaata cataaggt
//
LOCUS       DAZKFZ010000160        10511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000160 DAZKFZ010000000
VERSION     DAZKFZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.160"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(29..1030)
                     /locus_tag="ACHOQW_003550"
                     /pseudo
     CDS             complement(29..1030)
                     /locus_tag="ACHOQW_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1036..1383)
                     /locus_tag="ACHOQW_003551"
     CDS             complement(1036..1383)
                     /locus_tag="ACHOQW_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFP9574568.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(1413..2063)
                     /locus_tag="ACHOQW_003552"
     CDS             complement(1413..2063)
                     /locus_tag="ACHOQW_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574569.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(2079..2483)
                     /locus_tag="ACHOQW_003553"
     CDS             complement(2079..2483)
                     /locus_tag="ACHOQW_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9574570.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(2573..2710)
                     /locus_tag="ACHOQW_003554"
     CDS             complement(2573..2710)
                     /locus_tag="ACHOQW_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574571.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            2782..2985
                     /locus_tag="ACHOQW_003555"
     CDS             2782..2985
                     /locus_tag="ACHOQW_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9574572.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            3007..3357
                     /locus_tag="ACHOQW_003556"
     CDS             3007..3357
                     /locus_tag="ACHOQW_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP9574573.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            3368..3646
                     /locus_tag="ACHOQW_003557"
     CDS             3368..3646
                     /locus_tag="ACHOQW_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574574.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            3658..3900
                     /locus_tag="ACHOQW_003558"
     CDS             3658..3900
                     /locus_tag="ACHOQW_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP9574575.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            3897..4010
                     /locus_tag="ACHOQW_003559"
     CDS             3897..4010
                     /locus_tag="ACHOQW_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574576.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
     gene            4103..4519
                     /locus_tag="ACHOQW_003560"
     CDS             4103..4519
                     /locus_tag="ACHOQW_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574577.1"
                     /translation="MNAKEKNIINTLKIVSAEQDKLSRAAQKDNQHMAALYALTIAIA
                     TPEAAKVIEEQSKEIDTLKTQSTVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYL
                     ITPYHTKESELLTNLRLIERSQAVFIDDAQRAVFNA"
     gene            4543..4746
                     /locus_tag="ACHOQW_003561"
     CDS             4543..4746
                     /locus_tag="ACHOQW_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574578.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            4743..5009
                     /locus_tag="ACHOQW_003562"
     CDS             4743..5009
                     /locus_tag="ACHOQW_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9574579.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            5006..5305
                     /locus_tag="ACHOQW_003563"
     CDS             5006..5305
                     /locus_tag="ACHOQW_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574580.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            5628..5858
                     /locus_tag="ACHOQW_003564"
     CDS             5628..5858
                     /locus_tag="ACHOQW_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP9574581.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            5931..6296
                     /locus_tag="ACHOQW_003565"
     CDS             5931..6296
                     /locus_tag="ACHOQW_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574582.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            6303..9125
                     /locus_tag="ACHOQW_003566"
     CDS             6303..9125
                     /locus_tag="ACHOQW_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9574583.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            9202..10161
                     /locus_tag="ACHOQW_003567"
     CDS             9202..10161
                     /locus_tag="ACHOQW_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP9574584.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            10166..10480
                     /locus_tag="ACHOQW_003568"
     CDS             10166..10480
                     /locus_tag="ACHOQW_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP9574585.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         2922 a         2819 c         2427 g         2343 t
ORIGIN      
        1 atctattgtc actttttatc gccacttttt accctctatt gtcaaaaggg ttcaattcaa
       61 cagcagcctc taaatgacct ggagcaaaat gcgcataacg catagtcatt tttatatcgc
      121 tatgccccag tattttttgc aacacaagaa tatttccacc ccgcatcata aaatgactgg
      181 caaacgtgtg acgtagcaca tgagttaatt gcccatcagg aagctcgatc ttcgctctct
      241 taattgcagc gtcaaaagcc tcataacatg gtgaaaatag cgctcctcgt tttttaggaa
      301 gcatagcttg caattgaggt gaaatcggta cagtgcggtt cttctttcct ttagttttaa
      361 caaatgtgat tcgaccgggc agtacttgag attgctttaa tccttctgct tcactccacc
      421 gagcaccagt cgcaagccca atacggacaa caacccccaa atctttattc cgtgactcat
      481 cacacgcaat cagaaggcgt tcaatctcat ctacatacag aaacgccagt tccttttctt
      541 cctcacgaaa cttgcgaata ccagtcaggg ggttttcacc agaccactcc ccaaggcgct
      601 tcagttcggc aaaaacagca tgtagatatg actgctcgcg attaacggtt gcttcactaa
      661 gttttttctt ccccttggga ttccattctc ctgatagcct tctttcccga taagtagcaa
      721 acatattttt gtcaaactga gaagcaaatg gatctcccag cctggaacaa atcgcctcaa
      781 gtttgacttt gcgctctgca ccagaggaca aggttttacc gtacatctca aaccaacgag
      841 caatcaactc agaaagacga ggaccagaac catcttgaaa ctcgtctcca actctactat
      901 tcattaaacg gcgctcataa gagagcgcct cactttttgt cgcaaactgt ttacgaatgc
      961 gttttcccga tgccccgtag ggataacatt cacaaagcca tttaccttat ggaatcttac
     1021 gaaccgacat tttagttact tatcacataa atcaaatgca gccttagtga catcccccag
     1081 actctttttt aaccctgggg cggcatcatt atctagccaa aatggattat tgttatctaa
     1141 cggtaacgca ccaaatgttt taccttttat tcgagccaaa cctgtaagtg catataactt
     1201 attatcgtca aaattcatca cataaggatt accatcaaga cactgtaatt gaacctcatc
     1261 agtattaaat ggccataccc cattgaaact ctcacgttca atagttttaa aaggcattgc
     1321 gacggcggaa aaagaaaaca tagataaaaa agtaactaat agttgaacct tttttacttt
     1381 catatcatta cctcaattta gctcaagtaa gattacaaat taaaaaacgc cctagaaatg
     1441 acaccgccta ccaaaacccc tacgcagata aagaatatta tttcttttgg ataaagtcgg
     1501 attaattctg aagcacgaag tcggacttct ggtaaggtcg aactctctat gtggcttgat
     1561 gccgattgtt gctctaacca cgacaatgca gactgtaact gagaacgagt aagatcgttt
     1621 aaacgtcctg taccgaaatt gatatggcaa taccgcagaa gtttttgtcg aagtccacag
     1681 tcttcactgt tacgtagtaa taaacttaca agagccttac aggcatcatg atctttacat
     1741 cgctcaagca ttgcatgcag aaaactctcc gctgttttat attgatttac tgtcatatca
     1801 tcaataccag ctacaccaat ctccgcatgt actttttgcc aaataataaa cgcttcagta
     1861 ttgctagctt ctgcaatagc agcaaccaag ctatttagct ccttacgctg agccttaagc
     1921 aaagggcgat cgtcatcatc attattcgaa gggattgcga tattgacggt atgagaacca
     1981 tcatatcgct ctatctgaat attcttttcg tgaaaatcac gcccagcaac gcgattgttt
     2041 gaaccgtttg agttgacggc catgtcacct ccctactatc acctaccctt agtttcgtta
     2101 tagtcacgac cagcgatacg gttattacca ccagaaatat ttaactcacg tcctgatggc
     2161 tgagtttcct tttcactgat cgcacctttt aaagccccaa tcaccgcgtt tttcacatct
     2221 aacgaagctg ctcgaaagcg agtaatcaat tcctgctcat catcgttata agtttcaggt
     2281 gagtgaattc ccaacacaac atactgaaca tcaaggccaa aacgagacag cgctgccaaa
     2341 tacgcagcat caggaaagct atctcctttc tcatatctaa gctgagttag ctttttgact
     2401 ccaccaatgt cgctcatggc aacttgacta agtcccaatc tttccctttc ctcacgcaac
     2461 cgccgaccaa tatcattttt catacaaaaa ccttgacagg tatctttttt gataccaaaa
     2521 tgatttcacg agctattaga tgatcacaat ataccactat gaaacaagtt cttcacgata
     2581 ccagatcacg cattccgcgt aacaccgcca caggtccaag actggcactt cggctgtccc
     2641 tcgaggagcg agccgtcatt gatgaaatgg cagctaaaga acaacgctca tcctctaaca
     2701 tggcgcgcat gatctccctt cgcggcctag agctaaccca gaaagaacaa aacaaatctt
     2761 cctgatcagg aggctagtgg gatgtcaggt ataaccatca atatcaatgt gaatgccccc
     2821 tatgtatccc tgcagaaata tgcagagata acaggtatcc ctcttaatac atgcaaaaag
     2881 atgttggctg acggtcgaat tattatccgc cccaaacgcg ccaaaatgga aaagcctgaa
     2941 gtaaaccttg tggcgatgtt aaaagacgct ttggctaaca gctaaaacaa tgaacagagc
     3001 accatcatga aaaaaaacgc taataatcca tactccaaat ttcgtaatgg cgtagaacgc
     3061 catgtacacc acgtcgctac cagtgcatca cgtagtaaca gtcgctataa cctgaacgag
     3121 acgcatgcaa caccggatgg ccacgctgta aaacaaatcg gcgaacatgc ctggctgatt
     3181 gagaaagctg gaatcgtggt ccacaaatgc ccacgcaatc cgtttaccgg gaaccgcatt
     3241 tttgcattga actgcggcga caatcacttc gggcaggatt tcacattata cgaagcactt
     3301 cgcacggttg atcgtctgct tcgcgggcaa agttttatta aacaggctga tttataacag
     3361 gtgctttatg accaaagagc atgcacaagg tgtatttatc cgttttattg attttcgcgg
     3421 tgaactgtta ttacgcgcat cagccattga cggaattgtt ccatccgaaa aaaacgcagc
     3481 tacttacgtt tatctgaacg gtacgcgcct gactgtggag cttccgtacc agaccgtacg
     3541 agaaatcatt agcgaagctg aaaaggctcg tcaggttaat ggcgatgaac cctatatcga
     3601 aattatttgt atggattcag aagctgaaat tcagaaagca gattaaaggg cgttgcgatg
     3661 ggcaaagaat ataaaactct cattaacaaa gcacctgaac gattttattt tcgcttaagt
     3721 gcatcaggcg ctcatgctga acgtgcagcc cgtgactcat tgaccagggc aatccggagt
     3781 ttgtatgacg ttgcttttta cgctgatgat ctggatgcac ttaacgaact ttccgagctg
     3841 atctgtgccg cagaatgcgg ggaacacatt gaaccatata agctggggaa tatcgcatga
     3901 gtatatttat ctcatggctt gttctgatta tttcggtggc ctgcgccatt gggattatgc
     3961 gaattattca ttcagtaaaa aagattgaac gctttttcac tgacaaataa cagcttaaac
     4021 agaacaccag attaaacccg aaaacctgaa aactatccgc attcgcggag gtattcgcac
     4081 acgtaaataa cggagatata aaatgaacgc aaaagaaaaa aatattatca atacattaaa
     4141 aatagtgtca gcagagcaag acaagctgtc cagggcagcc cagaaagata atcaacatat
     4201 ggcagcgctt tacgcactga ccatcgcaat agccacccct gaagcagcca aagttattga
     4261 ggaacagagc aaagaaatcg acactcttaa aacacagtca acagttgcag ccatgaatcc
     4321 gtccagcatt ggacgctgca tttatattct tggttcagca atgatgctgc aatacaccat
     4381 tattgccgaa ctgcacggca aatacctgat aacgccttac cacacaaaag agtcagagct
     4441 tctgacaaat ctccgcctga tagaacgctc tcaagctgta ttcattgatg acgcgcaacg
     4501 tgccgtattt aacgcatagg gttactggac aaagggggcg caatggcaat taagcatttt
     4561 cccgtcgttc gctttacctc cagagggcgc gaatacgagg tcgacgaacg cctgattacc
     4621 actatcgaca aacatcgttc ggaaaaggat gcacaccaca tctacctcac tgacggcact
     4681 tacttctgcg ccaccaacgt ggcgcgagta aatcttatcc gacaggtaca ggagccacgc
     4741 agatgaccat tctggactac atcgctactc atccggggtg tagcggcgga gagatcgccg
     4801 cagcactgaa taccccaacc acagccatta atgctgagtt acgccgactt tggcgcggcg
     4861 gcttagtcat cagaacaaac cgcagcacag gtggtcgcgc tcgcaaaaca ggaggccagg
     4921 cttcttacca cgtaaacccg atgccgttcg ggtgtagcaa tccacttact cacatgttta
     4981 accagctact gaaggaagcc agagcatgag caccatcaac caccagaagc tacgcgaact
     5041 ggcatttgcc ctgcaacgaa tggcaacgcc tcaaaaatta ctggcatttc gcgcaatgct
     5101 ctcgccgtct gctgtgctgg cactgctgga tcagctggag cacgccagaa ccacggctcc
     5161 tgccattcgc ctcacgctcc atcatgaaat cgctgatttc tgcgcaacgc tgggggcacc
     5221 tggcgaaccg gaaacgccgg aagcaatgca gcaagagctg ctgcaacgca tcgataacgt
     5281 ttttgatttt tttctgaacc agtaagaaac cagaacatgc acacacaaaa aaaccgcttg
     5341 ccatgccgca atcggtcagg ttacatttcc gctgcacctc ataaaacggg tgccgggatt
     5401 ctcaacccga tacagagcaa agcgcataac cgcgctagcg gtttttttgt gcgtactgta
     5461 ttgccacgtc tttttcgcgt cagaattatg gcggggcgta cggggccgac ttcggtcggg
     5521 ccggattctt tgctctccgg tgttgagaac cctgtacgtc tcgccacccc gagattctca
     5581 actctggatg gtgagttatt tctatcaccg agcaaagagg ccacaccatg gcagaccgca
     5641 aacagcaccg cgctatcgcg gagcgtcgtc acatccagac tgaaatcaac cgcagacttt
     5701 cccgcgcatc acgcgtcgcg caaatcatgc acatcaatat gctgcatgag cgcagccacg
     5761 cactatcaaa catttattcc gcctctgttt tcagctatct ggcggatgat ctgcacgagt
     5821 ttcaacagct catccagcag caaaacaaac tccattaatt cctgttccgg gcctttcctg
     5881 caccttgcgg cgggaggcct tcgcacatct gtaacaagag gattgccgca atgattctcg
     5941 ccaacgactt tcttgaatac ctgctcaaca cagaacgtga tcttgccgct cgcgtgcgtg
     6001 atcgttatga catgtacctg aaatccctgc ctgtaccgca gctcgctgac ggaaagattg
     6061 ttattgatgg tcgctacatg attgacagcc acgagggaaa ttacaggctt taccgcattg
     6121 aaggtggcac cccgtccgtt attggcattt accagcgccc atcctctgca atcgtcgatg
     6181 tgattgccga cagcatccgc atcacacatc gccatgccga cacagaagac accgtgctgg
     6241 aaattcagcg gctggctaca gcctgccgcg acaccctgaa tggcatgacg aagtaaatca
     6301 ctatgacggc agagtacatc agggactggc aacaaccgcg ccacgcagtg gggcgtgaag
     6361 gaacggggat ccccgctcct gaatccgcgc tttcctcctg gctggatgcc taccgggtag
     6421 agaacgagcg ccgccaggaa atggctgatg cggcgttctc cgcaacgccg ctgggcaacc
     6481 tgattaataa aagcctggac gcacaggaaa aacaggacaa aaccatcaca ctggcaggag
     6541 acgccagaaa acaggcacgc ggtgcggtgg atgaagccat ggcctcgctg cgcctgctgc
     6601 cgtcctatct gcgcgatccg cttattcgcc acctctcctt cctgcgcaaa aaacaggaag
     6661 ccgatcgtca gaaaggcaaa aagagctggc aggctgaacg ctacgcgcgc ggaaacctgc
     6721 gcaaaatatt cgaacgtctg gagcgcaccg atcaccgctg gctgacacag ggttatcgct
     6781 cccttgccgg acgcgaacgc ctggacgatt tgctttacct gccgcagctc aacaaacacc
     6841 agatacagac gctggccacc atgacggcgg cgatgttcag cagcaccttc gaaaaactct
     6901 gcgatggctt tggcgcgacc gatggcgaac tgaccatgga tgtaacgctg aaggcgtatc
     6961 agatgctggc ccgcatggcg ttacacctgc acgccatgcc tccacattat gacgcactga
     7021 caacagacaa agaccggagg aacgaaccgg acacggagct gctgccgggc gcaatccttc
     7081 gcctgacctg tgcggaatgg tggaaacgca aactgtggct gttacgttgc gagtggagag
     7141 aagaacaact ccgcgccgcc tgtctggttt ccagaaaaac atcgccctat ctgagccagg
     7201 acgcgttaag cgagtttcgc gcacagcgcg agaaaacacg cgatttcctg aaaagtttca
     7261 tgctggaaaa cgaagacggg ttcacgattg atctcgagac agtgtattac gcgggagtaa
     7321 gtaacccggt tcaccgtaag gcagaaatga tggccaccat gaaggggctg gaacttctgg
     7381 ccgaagcccg tggcgacaaa gcggtgtttc tgactgtcac ctgcccgtca aaataccacg
     7441 ctacaacaga gaacggtcat ccgaatccca aatggaacgg ggccaccatg cgcgactcca
     7501 gcgattacct ggttaacacg ttttttgcgg cggtccgcaa gaaactgaac cgcgacggcc
     7561 tgcgctggta tggcatccgc acggtggagc ctcaccatga cggcaccgtg cactggcata
     7621 tgatggtctt tgctcatccg gaagaaatcg acaccattgt gtcccacacc cgcgatattg
     7681 ccattcagga agatcgtcac gagctgggcg atgatattac tccgcgcttt aaggcggagt
     7741 atgtcgacgg ctcaaaaggc acgccaacca gctacatcgc cacctacatc ggaaaaaacc
     7801 tggacagccg cgccgtggat ggcatcgacc cgaaaacagg caaaccacgc gttgaccacg
     7861 aaaccggaaa atcaatgacc gagagcgtgg aacgcgccat tggctgggcg cgccttcacc
     7921 gggtccgcca gttccagttc tttggcatcc cctcccgtca ggtgtggcgt gaactgcgtc
     7981 gccttgccag ccagatggca cgcaacccgg aaggcccgca acggctgaag gatgacgcaa
     8041 tggatgcggt tcttgctgcc gctgatgccg gatgttttac cacctacatt gagaaacagg
     8101 gaggcgtact tgttccacgc aaggactacc tgattcgcac cgcctacgac ctcgcagatg
     8161 agctgaacga ttacggcgaa cagagcgtac agatttacgg gatctggtca ccactcatcg
     8221 gggaatcctc ccgtgtgtgc acgcacccgg ataactggaa gctggtaaga cgtaaaccgg
     8281 gagtagaaga cagcgcccgc gaaaatggtt ttgaccttca gggcggccct gccgcccctt
     8341 ggactcgtgg caataactgt ccccgtgtac aggaaacgga caacaacggg acagaacagc
     8401 cggaagaacg gccagcaccg tggccgcagc ttcctgacgg cgttgacgtg aacgaatgga
     8461 tgcgctcact gaaacggcac gaacgccggg cgctgatgcg ttcgcttcgt gacaaacagg
     8521 caaaaaacag cagtgatgaa atgcagagct ggacacagag ccgcaaacag cagcggcctt
     8581 tgcctgataa ccacgaatta ctcgctaaag aatggcggga gtctgctgaa tctctcggcc
     8641 tgcatatcgg tgaacaacag atgcagcacc tgttacgggg cggcagtctg tacgttgacg
     8701 gcagcatcat tgcaccgcag ggatttgaaa ttgtacgcaa accggatacc cgcccggaca
     8761 gccgaatcac gcagctctgg cagcgcctga gccgtaatca tggcgtaagc agcacggaga
     8821 tccgccataa cccggtcgcc agctatctgg cacagctggg ggcatcagac cctgaagccg
     8881 ccgcacgcct ggcatccaca cttcagcagg accagaacac catgaaaaca cccgttaccg
     8941 tgctttctga catgctgcgc gccatccgcg acgcagagca cgcacagaga atcagtgaaa
     9001 ccactgaacg cgccagccgc aaagcagacc tgctgcgggg tggcctgacc agtggaaaca
     9061 aaaaacagac agaaacggga ctcacaaatc ccgtaaatga gcaaaaaacg cgccgcgata
     9121 tatgaagcgc gcacaaaaca ggcaaaagcg ggatttaaaa atcctgtaac cgattaatta
     9181 atcaacataa ggaaaagcga catgaaaatt tgtatcgacg acggctccac caacatcaag
     9241 ctggcatgga ctgagaacgg cgaacgccgc aacgccatca gcccgaacag cttcaagtcg
     9301 gaatggtctg cgccattcgg tggcacgcag cccgcgaact acatgcttga tggcgtgcgc
     9361 tatggttttg atccggtcag cgatcgcttt gtccagacga ccgacacgca ataccagtac
     9421 agcgatgtga atgtaattgc cattcatcac gcgctggtca aatcaggcat cacaccacag
     9481 gaagtggatg tggttgtcac cctgccactg agcgagtatt tcgatacaaa cgcacagccg
     9541 gacatggcca acatcaaccg caaaaaagcg aacgtcatgc gcccggtgga gtaccagaac
     9601 ggcgaagcat tcactatccg taacgtacgg gttatgcctg aatccattcc tgctggcttt
     9661 aaggcactgg ctgacatgag tccgtttgaa tccctgctga ttgtggattt gggcggaacc
     9721 acgctggatg tggcaaaggt tcaggggcaa ctggcaggta tcagccaggt gttttgcgat
     9781 ccacacgtag gcgtttctct gatggccgat gccgtactgt cggtgatggc cactaacggt
     9841 atgcgcacca gtcaccacat cgccaatacc attatcgaac atcgccatga tgaagcctgg
     9901 ctgcgccagc acatccacaa tgacgcgcat tacgccagcc tgatggcggt tattcgtgaa
     9961 aaggaagaaa cactgaaaca acgcgtgatc cgcgcgctgg cggttttttc gggttacggg
    10021 cgggtgatgg ttgtcggtgg cggggcggag attgtggcaa ccgctatccg cgaagcctgc
    10081 ggagttaatg cgactttcat cgcggacggg gtgccacagt ttgctctggt taatgggttg
    10141 tacgcaatga acaaggagta aaccaatgac gacaccaacc agacggataa gtttctatct
    10201 gaagcccgcc gccgtcaaga gtgaacagga ggcgtgcaat tacctcgata gcctgccagc
    10261 ctccgaacgc agccgcgcgc aacgcgcggc ctttctggct gggctagcac tcataaagcg
    10321 caacccagca tttgcctact ggatggccga atggccagag gacaacctcc ccttcaatac
    10381 cgttagcatc aaggaaacat accaggctaa tcagcctagc ggagttgaat gcaattttta
    10441 taaaataaaa aagaatattc agaccttatt cccagaataa tctgataatt gtatgtgaac
    10501 taataaaata c
//
LOCUS       DAZKFZ010000161        10506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000161 DAZKFZ010000000
VERSION     DAZKFZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            36..362
                     /locus_tag="ACHOQW_003569"
     CDS             36..362
                     /locus_tag="ACHOQW_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9574586.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            515..664
                     /locus_tag="ACHOQW_003570"
     CDS             515..664
                     /locus_tag="ACHOQW_003570"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9574587.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            686..3220
                     /locus_tag="ACHOQW_003571"
     CDS             686..3220
                     /locus_tag="ACHOQW_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9574588.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            3232..4302
                     /locus_tag="ACHOQW_003572"
     CDS             3232..4302
                     /locus_tag="ACHOQW_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9574589.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            4330..5412
                     /locus_tag="ACHOQW_003573"
     CDS             4330..5412
                     /locus_tag="ACHOQW_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9574590.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            5624..6664
                     /gene="pstS"
                     /locus_tag="ACHOQW_003574"
     CDS             5624..6664
                     /gene="pstS"
                     /locus_tag="ACHOQW_003574"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9574591.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6751..7710
                     /gene="pstC"
                     /locus_tag="ACHOQW_003575"
     CDS             6751..7710
                     /gene="pstC"
                     /locus_tag="ACHOQW_003575"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP9574592.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7710..8600
                     /gene="pstA"
                     /locus_tag="ACHOQW_003576"
     CDS             7710..8600
                     /gene="pstA"
                     /locus_tag="ACHOQW_003576"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9574593.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8783..9556
                     /gene="pstB"
                     /locus_tag="ACHOQW_003577"
     CDS             8783..9556
                     /gene="pstB"
                     /locus_tag="ACHOQW_003577"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9574594.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9571..10296
                     /gene="phoU"
                     /locus_tag="ACHOQW_003578"
     CDS             9571..10296
                     /gene="phoU"
                     /locus_tag="ACHOQW_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9574595.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         2679 a         2496 c         2653 g         2678 t
ORIGIN      
        1 tgttttttgc tgttactttg attacgcaga tgtcgatggc tacggctggt attgttgttg
       61 ggggaactcg cgttattttt tatggtgaca aacctgatac cacaatctct atttttaata
      121 aagaaacgaa ccttccttat ctattgcaga catgggttga tccatttaat aaagaggata
      181 aaagcaaacc gccattttct gtgatacctc ccgtttctcg cattgaacca cagcaagaga
      241 aaatattacg cattttaaaa acggctggcg agttaccaaa agatcgggag tccgtattct
      301 ggttgaacgt gaaaaacatc cctccttcaa ttgataaaaa aataccaatg ttcttgagat
      361 agccataaaa acaagaatta agttattctg gcgaccagcc gcggtaaaga taatccctga
      421 gcaggctatt aaaaatgtta aatggtcgcg gcagggtaat aatctcatcg tcgaaaatcc
      481 aaatccgatc catattaatg taatgagtgt gacggtggat gataaagacg tgtcgctaaa
      541 tatgatccgc ccttttgaaa tattgacatt accaattccg gctggcgtgg caggaaaatc
      601 gctcgtttgg cgctttatta atgattacgg tgcaattagt caaccactaa agaaaaacct
      661 ttaagcgccg gataaggata tatcgatggc ctcttcgcat cttttcatta cgcttgcatc
      721 gggcatatgt ctgctctgtt ccatatctgc ttttgcccgg gatagcttgt tcaaccccag
      781 attactggaa ctggatcatc ctgcggataa tattgatatt caccagttca accgttcgaa
      841 taccttacct gcgggaacat acaaagttga tgtgatgatc aacggcatgc tcttcgaacg
      901 ccaggaagtt aaattcgccc aggataaccc tgatgctgaa ctccacccat gctacgtggc
      961 gataaaaaac gtgctggcga cctatggtat aaaagttgat gcgataaaat ctctggcgaa
     1021 tgttgatgac aaaacatgcg taaatccagt tccgctgatc gacggggcta cctggttact
     1081 ggacgccagt aaacttgcat tgaatattac tattccgcaa atttatctca acaatgcagt
     1141 taatggttat atcagccctt cccgttggga tcaggggatc aatgccatga tgatgaatta
     1201 tgatttttcg gcatcgcata ccatccggtc aaattatgac gacgacgatg acagttatta
     1261 tctgaatttg cgtaatggta ttaatttagg cgcatggcgt tttcgtaatt acagcaccct
     1321 gaattcttat gacggtaatg tggactacca ttccgtcagt aattacattc agcgcgacat
     1381 catggcatta cgtagccaga ttatgattgg cgatacctgg acggcaagcg atgtatttga
     1441 tagtacacag gtgcgtggcg tgcggctgta taccgatgac gatatgttgc cctccagcca
     1501 gaacggcttt gcgccagtgg tacatgggat tgcgaaaact aacgccacgg tgatcatcaa
     1561 acaaaacggc tacgttattt atcaatcagc cgtaccacag ggcgcatttg ccctcaccga
     1621 cttaaacacg accagtagcg gcggcgatct cgatgtcact atcaaagaag aagatggcag
     1681 cgagcagcac tttattcagc catttacttc actggccatt ctcaagcgtg aaggtcagac
     1741 cgatgtagac cttagcattg gagaagtgcg cgacgaaagc ggctttacgc ctgaggtctt
     1801 gcagttacaa gcaatgcacg gtttcccttt gggaataact ttgtatggcg gaacacaatt
     1861 ggcaaatgat tacgcttctg ccgcgctggg tattggtaaa gatatggggg cgctgggcgc
     1921 gatttctttt gacgtgactc atgcccgctc gcagtttgac tacgacgata atgagagtgg
     1981 tcaatcgtat cgttttctct attccaaacg ttttgaagac accaatacca cctttcgtct
     2041 ggtgggttat cgctactcta tggaggggtt ctacaccctc aatgaatggg tgtcgcgaca
     2101 ggataatgat tctgatttct gggtaacggg caaccgtcgc agccgcttcg aaggcacctg
     2161 gacgcaatct ttcacgccag gctggggcaa tatttattta acattcagtc gacaggaata
     2221 ctggcagacc gatgaggtcg aacgtttatt acagttcggc tataacaaca actggcgaaa
     2281 catctcctgg aacgtttcct ggaactatac ggactcgatc aagcgctcat tgggcaacca
     2341 tcatgatgat aacaatgatg atttcggcaa agaacagatt ttcatgttct caatgtcgat
     2401 accgctatcg tgctggatgg aagacagcta cgtcaactat tcgttaacgc aaaacaacca
     2461 ccatgaaagc acgatgcagg tcggtctgaa cggaacgatg ctggaagggc gtaacctgtc
     2521 ttataacgta caggaatcgt ggatgcactc tcctgatgac tcctacagcg gcaatgccgg
     2581 aatgacctat gacgggactt atggctcggt caatggtagc tattcctgga gccgtgactc
     2641 ccaacatttt gattatggcg ccagaggcgg cgtgctggtg catagtgacg gagtgacctt
     2701 ctcgcaggaa ctgggcgaaa cggtggcatt ggtcaaagcg ccgggcgcag aaggcctgtc
     2761 cattgaaaac gccaccggga tttctaccga ctggcgtggt tataccgtaa aaacgcagct
     2821 tagcccgtat gacgaaaacc gcgtggcatt gaacagcgac tatttctcca aagccaatat
     2881 tgaactggaa aacaccgtca tcaacctggt accaacgcgc ggtgcggtgg tgaaagccga
     2941 atttgtcacc catgtcggtt atcgcgtgct atttaacgtc cgccaggtca acggtaaacc
     3001 aataatgttt ggcgcgatgg caaccgcctc tctcgaaacg ggcacagtca ccgggattgt
     3061 cggtgataac ggcgaactgt atctctccgg gatgcctgaa aaaggcgagt ttttattgag
     3121 ttggggacaa gctgcggatg aaaaatgtaa ggcggcctat cacatcaccc ataaacctga
     3181 tgataccagc ctggttcaaa tggatgcgat ttgtcgctaa aggaagcatg aatgaaaaaa
     3241 atattatcag ggttgattct gctgctttgc tgtccttatg gtttcgccgc taatggtgat
     3301 ggcgcaacgc acatgtcaaa tttatcattt ggtccgctga cggtggcagc ggcgaataac
     3361 cactccggat acaatatttt cgaggcactg agcaacacga ctggaacata cccggtgcgc
     3421 tgtcactgtg atgacacgca tggcggaccg ggccaacaaa cagcattttt tcctatcttc
     3481 tacacgggag atgccgcacc ggggcttgtg cttgagcgca ctcttaatgg gttaaattac
     3541 tatgctctga atgattattt atcggtcggc gtgacgattt ttattattaa taaccaatat
     3601 gcggccattc cttttgaaca cttatccaac caatccacct caccgcaaca tacctgtgga
     3661 gcaggtaata atgggagcac tgtaaatctg gattcagggc gctcggcaaa attatctttc
     3721 tatgttcggc attctattac tggcacggtg acaataccca caacggaagt cgcctggttg
     3781 tacgcgggca tgtccgatca ttttcccaaa acgacccccg tttctaaagt gacaattcgc
     3841 ggacaactaa cggccccgca gaactgtgag ttaacgccaa atcagagcat cgatgtcgat
     3901 tttcaaaaaa ttaatagcgc tgagttctcc tcaacggcgg gttcaattat tgcagaaaga
     3961 aagattaaaa ccgaagtcac agtatcctgt accgggatgg aagacgtaag gtccacggag
     4021 gtggtgagtg cgtcgatgat tgcggcaaac agaagtgccg atgccaccat gatcgtgacg
     4081 agtaatccgg atgtgggaat taagattttt gataagaacg accgtccagt gaatgtggat
     4141 gggggcaact tacccgctga tatgggtgct attagtcgat taggaaaaac cgatggtagc
     4201 gtaacgtttt attcagcgcc cgccagtctg acgggcgcaa aaccagcgcc tgataatgga
     4261 tttaccgcta cagccacgct ggttattgaa tttactaact aatcttgtgt tagtaaaagg
     4321 aataatttca tgaaaaaatg gcattatata ttttgcataa ttctctttca tttagggtta
     4381 ccgtgcgggt atgcggcaaa tgatggcacg tgtgcaacaa gaggcggcac acatacatta
     4441 agccttaatt ttcctctgac aacggtcagt gcagcaaaca atgtgcctgg aaatacatta
     4501 atagatattg ctaatgcaac atcttctgaa aattatagcg ttctgtgtaa ctgtgattca
     4561 aaacatagca atggcgctta tcacgaaata tattataccg cagaccctgc tcccggtatg
     4621 gtttatagca ccaccgcaag tggtcttgct ttttactatc ttaacgaata tgtcgatgtg
     4681 ggaacaaaaa tatctgtgct aaatgcgggg tatacggcag ttccttttga acatgtttcc
     4741 aaccaggcaa ctacaacaga tcacacttgt cagggaaaca aaactacagc ggttggcgtg
     4801 agcctgaaaa ctggagcaga tgcgaagatt tcatttcgta ttaaacgttc aataaatgga
     4861 acggtagtaa tacctatcac cgatattgca ttgctgtatg ccaacatatc cagcaccacg
     4921 acccgtggtg aggcgattgc aaaagttcga atttcaggca gtttgaccgc accacagtct
     4981 tgtcagataa atgcaggaca ggtgatttat tttgattttg atactattcc tgcgtccgaa
     5041 ttttcatcta ccgccgggca agccattact tcacgaaaaa tcactaaaac agtgagtatt
     5101 gagtgtacgg ggatggggta tgagcgtacg cagaaagtcg atgcttcttt tacggggacg
     5161 aaccgaagca gtgacgatac gatggtggcg acagacaatg ctgatgtcgg gatcaaaatt
     5221 tacaataaat cgaatgctga agttagcgtc aacaacggca agttacccgc agacatgggc
     5281 aacacgacca tttttggtcg taaaaatggt tcggtaactt tttcggcagc acctgccagc
     5341 tttaccggtg cccggcctca gcccggcgtt tttaacgcta ccgcgacctt aaccattgaa
     5401 tttgtaaact aaccttttgc ggctgtttta tgacagccgt tcatatttcc tctcctttca
     5461 tgaaaaaatg ctgatatttt tccccttcaa tatcaataag taataacttt cctttgggtg
     5521 tcatcaaact gtcacattcc ttacatataa ctgtcacctg tttgtcctat tttgcttctc
     5581 gtagccaaca aacaatgctt tatgaatcct cccaggagac attatgaaag ttatgcgtac
     5641 caccgtcgca actgttgtcg ccgcgacctt atcgatgagt gctttctctg tgtttgcaga
     5701 agcaagcctg acaggtgcag gtgcaacctt ccctgcgccg gtgtatgcca aatgggctga
     5761 cacttaccag aaagaaaccg gtaataaagt taattaccag ggtatcggtt cttccggtgg
     5821 cgtaaaacag attatcgcca ataccgttga ttttggtgct tctgacgcgc cgctgtctga
     5881 cgaaaaactg gctcaggaag gtctgttcca gttcccgacc gtgattggcg gcgtggtgct
     5941 ggcggttaac attccaggtc tgaagtctgg tgagctggtg ctggatggta aaaccctcgg
     6001 cgatatctac ctgggcaaaa tcaagaagtg ggatgatgaa gccatcgcca aattgaatcc
     6061 gggtctgaaa ctgccttcac agaacattgc tgtagtgcgc cgcgcagatg gctccgggac
     6121 ttccttcgtc ttcaccagct acctggcgaa agtgaatgaa gagtggaaaa acaacgttgg
     6181 tactggctct accgtaaaat ggccgatcgg tctgggtggt aaaggtaacg acggtatcgc
     6241 tgcgttcgtt cagcgtctgc cgggtgcaat tggttacgtt gaatatgctt atgcgaagca
     6301 aaacaacctg gcgtacacca aactgatctc cgctgatggt aaacctgttt tgccgaccga
     6361 agaaaacttc gctaatgcag caaaaggtgc agactggagc aaaaccttcg ctcaggatct
     6421 gaccaaccag aaaggcgaag atgcgtggcc tattacctct accacgttca ttctgatcca
     6481 caaagatcag aagaaaccag aacaaggcac agaagtgctg aaattcttcg actgggcgta
     6541 caaaaccggg gctaaacagg cgaacgacct ggattacgcc agcctgccgg atagcgtagt
     6601 tgaacaggtt cgcgctgcgt ggaagaccaa tattaaagac agtagcggca agccgctgta
     6661 ctaataaaac tccaggccgg ataaggcatt aaacggctta tccggcatta caaaatgact
     6721 ttgtaaacgc gtttaactga agagtaactt atggctgcaa ccaagcctgc ttttaaccca
     6781 ccgggtaaaa agggcgacat aattttcagc gtgctggtaa aactggcggc gctgattgtg
     6841 ctattgatgt tgggtggcat tattgtctct ctgatcatct cctcatggcc gagcattcag
     6901 aaatttggtc tggctttcct gtggaccaaa gagtgggatg cgccgaacga tatctacggg
     6961 gcgctggtgc cgatctacgg tacgttggtg acttcgttta tcgcgctgct gatcgccgtc
     7021 ccggtgagtt tcggtatcgc cctgttcctg actgagcttg cgcctggctg gctgaaacgc
     7081 ccgctgggta tcgccattga gctgctggca gccattccaa gtatcgttta cggcatgtgg
     7141 ggcctgttta tctttgcgcc gctgttcgcc gtttacttcc aggagccggt cggcaatatc
     7201 atgtcgaata tcccgattgt cggcgcgctg ttctctggcc cggcatttgg tatcggtatc
     7261 ctcgcggcag gcgtgatcct cgccatcatg attattccgt acattgcggc ggtaatgcgc
     7321 gatgtgttcg aacaaacccc ggtgatgatg aaagagtcgg cctacggtat tggctgcacc
     7381 acctgggagg ttatctggcg tatcgttctt ccgttcacca aaaatggtgt tatcggcggc
     7441 attatgctgg ggctgggccg cgcgcttggt gaaaccatgg cggtgacctt tattatcggt
     7501 aacacctacc agctcgacag cgcctcgctg tacatgccgg gcaacagtat cacctctgcg
     7561 ctggcgaacg aatttgccga agcggaatcc ggtctgcacg ttgccgcact gatggaactg
     7621 ggcctgatcc tgtttgtgat taccttcatc gtcctcgccg catcgaagtt tatgattatg
     7681 cgcctggcta agaatgaggg ggctcgctaa tggctatggt tgaaatgcaa accactgcgg
     7741 cgctggcgga atcccgccgc aaaatgcagg cgcgtcgccg cctcaaaaac cgtattgcac
     7801 tgacgctctc aatggcgacg atggccttcg gtctgttctg gctgatctgg attttaatgt
     7861 ccaccatcac ccgcggtatc gacggtatgt cgctggcgct gttcactgaa atgacgccgc
     7921 cgcccaatac ggaaggtggt ggtctggcga acgctctggc aggtagcggg ctgttaattt
     7981 tgtgggccac ggtattcggt acgccgctgg gcattatggc ggggatttat ctggctgaat
     8041 atggtcgtaa atcctggctg gcagaagtga ttcgcttcat taacgacatt ctgctctctg
     8101 cgccgtcgat tgtggttggt ctgtttgttt acaccatcgt ggtggcgcag atggagcact
     8161 tctccggttg ggcgggtgtg attgcgctgg cgttgttgca ggtgccgatt gttatccgca
     8221 ccaccgagaa catgctgaaa ctggtgccgg acagcctgcg tgaagcggct tatgcgctgg
     8281 gtacaccgaa gtggaagatg atctccgcga ttacgctgaa agcgtcggtg tccgggatca
     8341 tgaccggtat cctgctggcg attgcccgta ttgctggtga aaccgcgccg ctgctgttta
     8401 ccgcgctctc caaccagttc tggagcacgg acatgatgca gccgatcgcc aacctgccgg
     8461 tgacgatctt taagtttgcg atgagcccgt ttgcggaatg gcagcaactg gcctgggccg
     8521 gggtattgat cattaccctg tgcgtactgc tgctgaacat tctggcgcgc gttgttttcg
     8581 cgaagaataa acacggttga tattgctgac acggttttcc cctcacccta accctctccc
     8641 cagaggggcg aggggaccga ccgagcgcct ttttgactct gtacacggtt aacactttgc
     8701 cggatgcggc gtgaacgcct gatccggcct acggtaagcc tgattcgcga agtgcatcag
     8761 gcacgatgag gaaaagattg caatgagtat ggttgaaact gccccgagta aaattcaggt
     8821 tcgtaatttg aacttctact acggcaaatt ccatgccctg aaaaacatca acctggatat
     8881 cgccaaaaac caggtaacgg cgtttatcgg gccgtccggc tgcggtaaat cgacgctgct
     8941 gcgtaccttc aacaaaatgt ttgaactgta cccggaacag cgtgcggaag gtgaaattct
     9001 gctcgatggc gacaacatcc tgaccaactc tcaggatatc gccctgctgc gtgcgaaagt
     9061 gggcatggtg ttccagaaac cgacgccgtt cccgatgtcc atctacgaca acatcgcctt
     9121 tggcgttcgt ctgtttgaga agctctctcg tgccgacatg gacgagcgcg tgcagtgggc
     9181 attgaccaaa gccgcattgt ggaacgaaac caaagataaa ttgcatcaga gcggttactc
     9241 tctctctggt ggtcagcaac agcgtctgtg cattgcgcgt ggtatcgcca ttcgcccgga
     9301 agtgctgctg ctcgacgaac catgttcggc gctcgacccc atctccaccg ggcgtattga
     9361 agagctgatc accgaactga agcaggatta caccgtggtg atcgtcaccc acaacatgca
     9421 gcaggctgcg cgttgttccg accacacggc gtttatgtac ctgggcgaat tgattgagtt
     9481 cagcaacacg gacgatctgt tcaccaagcc agcgaagaaa caaacagaag actacatcac
     9541 cggtcgttac ggttgattca ggagtgcgtt atggacagtc tcaatcttaa taaacatatt
     9601 tccggccagt tcaacgccga actggaaagt atccgcacgc aggtgatgac catgggcggc
     9661 atggtggagc agcagctttc tgatgcaatc accgcgatgc ataaccagga cagcgatctg
     9721 gcgaagcgcg tgatcgaagg cgacaagaac gtcaacatga tggaagtggc gatcgatgaa
     9781 gcctgcgtgc gcattattgc caaacgccag ccgacggcga gcgacctgcg attggttatg
     9841 gtgatcagta aaaccattgc cgaactggag cgtattggcg acgtggcgga caaaatctgc
     9901 cgtactgcgc tggagaaatt ctcccagcag catcagccgt tgctggtaag tttggagtcg
     9961 ctgggccgtc atactatcca gatgctgcac gacgtgctgg acgcgttcgc gcggatggac
    10021 attgacgaag cggtacgtat ttatcgtgaa gataaaaaag tcgatcagga gtacgaaggt
    10081 attgttcgtc aactgatgac ctacatgatg gaagattcgc gcaccattcc gagtgtactt
    10141 accgcgctgt tctgtgcgcg ttctatcgaa cgtattggcg accgctgcca gaatatttgt
    10201 gagtttatct tctactacgt gaagggtcag gatttccgtc atgtaggcgg tgatgagctg
    10261 gataaactgc tggcgggaaa agaccccaaa gagtagtgct aactgtaggc cagataaagc
    10321 tttttgccgc atctggcaat cagcccggtc atacatagtt accgggctga tacactttct
    10381 cactgaaata ttgccacaca aactcacctc gaaactctcc ccagtacatt gaatatacat
    10441 tatttcattt gaattatttt acttttaatg tagtgcctcg ctataaaaac atatttgtta
    10501 ttcttt
//
LOCUS       DAZKFZ010000162        10431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000162 DAZKFZ010000000
VERSION     DAZKFZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            50..5291
                     /gene="iatD"
                     /locus_tag="ACHOQW_003579"
                     /pseudo
     CDS             50..5291
                     /gene="iatD"
                     /locus_tag="ACHOQW_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(5448..6043)
                     /gene="kptA"
                     /locus_tag="ACHOQW_003580"
                     /pseudo
     CDS             complement(5448..6043)
                     /gene="kptA"
                     /locus_tag="ACHOQW_003580"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            6248..6931
                     /gene="yjiH"
                     /locus_tag="ACHOQW_003581"
     CDS             6248..6931
                     /gene="yjiH"
                     /locus_tag="ACHOQW_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP9574596.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            6928..7389
                     /gene="yjiG"
                     /locus_tag="ACHOQW_003582"
     CDS             6928..7389
                     /gene="yjiG"
                     /locus_tag="ACHOQW_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP9574597.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            7402..8574
                     /gene="iadA"
                     /locus_tag="ACHOQW_003583"
     CDS             7402..8574
                     /gene="iadA"
                     /locus_tag="ACHOQW_003583"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9574598.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            8696..9550
                     /gene="hypT"
                     /locus_tag="ACHOQW_003584"
                     /gene_synonym="qseD"
     CDS             8696..9550
                     /gene="hypT"
                     /locus_tag="ACHOQW_003584"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP9574599.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(9543..9935)
                     /gene="iraD"
                     /locus_tag="ACHOQW_003585"
     CDS             complement(9543..9935)
                     /gene="iraD"
                     /locus_tag="ACHOQW_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP9574600.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(9932..10015)
                     /gene="idlP"
                     /locus_tag="ACHOQW_003586"
     CDS             complement(9932..10015)
                     /gene="idlP"
                     /locus_tag="ACHOQW_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP9574601.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(10131..10274)
                     /gene="ytiD"
                     /locus_tag="ACHOQW_003587"
     CDS             complement(10131..10274)
                     /gene="ytiD"
                     /locus_tag="ACHOQW_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFP9574602.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         2765 a         2617 c         2546 g         2503 t
ORIGIN      
        1 ccgtgctaga aaaaacaagt ttaattctgt tttttcacgg aaggtcacta tggcttcgac
       61 aaatgcgcat caaataaaaa ataatgatca aaactcttta tgtggtctgg gtgacaaaat
      121 acgccgcctg accgccggag tttgtttatt tacacaattt tttttccccg tcatggcgac
      181 ggcacaaaat gtggtacatg ctaaacctca gacgacagtt tcatctgccc ccccctctac
      241 tagaaaataa tactgtgcct tacacgcttg gtgcgctgga atcagcgcaa agcgttgcta
      301 atcgcttcgg tatttcactg gaggagcttc gtcgtcttaa tcagttccgt acttttgctc
      361 gcggctttga taacgtgcgc cagggtgaag aactggatgt tccggcaaca acctcgcaga
      421 aaagccatga gcaacaaaat gccgtaccgc ctgcgaatgg cgaaaacacg ctggagaatc
      481 aaatagccag cacctcgcag cgagttggca ctctgctttc acaagatatg aatagtgagc
      541 aggccagcgg catggcgcgt ggttgggcgt cttcagaagc ctcaggcgcg atgactgatt
      601 ggttaaataa ctttggtact gcgagaatct ctctgggtgt ggatgaagat tttagcctga
      661 aaaattcgca attcgacttc ctgcatccgt ggtatgacac acctgattat ctgctcttca
      721 gccagcatac ccttcaccga acagacgatc gtacccagat caacaccggt ttgggctggc
      781 gtcatttcac ctccagctgg atgtcaggca tcaacctttt ttttgaccac gacctgagcc
      841 gctatcactc ccgcgcaggg cttggcgcag aatactggcg tgattatctg aagttgagca
      901 gcaacgctta tatcggcctg accggctggc gtagcgcacc agaattggat aacgacttcg
      961 aagcccgccc ggccaacggc tgggatttac gcgcggaagg ctggttacct gcctggccac
     1021 aactgggggg aaaactggtc tatgaacaat actatggcga tgaagtggcg ctgtttgaca
     1081 agaatgatcg tcaaagtaac ccccatgcta ttaccgcagg cctcaactat acccccttcc
     1141 cgcttctgac tctcagtgcg gaacagcgtc aggggaagca aggtgaaaat gacacacgtt
     1201 ttgccgttga tctgacctgg caacccagca gttcaatgca gaaacagctt aatccggacg
     1261 aagtggccgg acggcgcagt ctggccggta gtcgttatga cctgattgat cgcaacaaca
     1321 acatcgttct ggaataccgc aagaaagagc tgattcgcct gagtctgctg gatccggtga
     1381 aagggaagtc tggagaaata aaaccgctgg tttcctcgct acagaccaaa tatgccctta
     1441 aaggctataa catcgaagcc gctgcgctgg aagctgccgg aggtaaagtc agcacgtctg
     1501 gaaaagatat cacggtcacg ctgccaggtt accgcttcac taacacccca gaaaccgata
     1561 atacatggtc gatagacgtt accgccgagg atgtaaaagg taacctgtca cggcatgaac
     1621 aaagcatggt agttattcag gctccgacat taagccagaa agattctctg ttatccgtca
     1681 atccgctaac cgtggctgca gataaaaaat cgacgaccac attgaccgtt actgcgcacg
     1741 attccgacgg aactccggtg ccggggctgg cgctgcaaac ccgcagtgaa ggcgttcagg
     1801 atatcaccct gtctgactgg acagataacg gtgatggtag ttacacacag atactgaccg
     1861 ccggaacgac atcaggttca gtaacactga cgccgcaaat taacggtgag agtgcggtaa
     1921 aagaatccat cgtcgttaat atcgtccctg ttgtctcatc ccgcgaccat tcatcaataa
     1981 caattgataa cgtatcgtat tatgccggag acgacatcaa ggttagggtg gaactgaaag
     2041 acgatagcaa tcaaccggtt gcatatcaaa aagaggaatt ggtaaaagcc gttactgtcg
     2101 aaaacagcaa acctggcgcc acgattgtct ggcacgaaga gcagccgggg gtttatgccg
     2161 cgaattatcc ggcctataag caagggactg cactaagggc acaacttagc cttcacaact
     2221 ggaatgctcc actgcaatcg catatttata acattgaggc aaaccagaat aaggctcgcg
     2281 ttgccacatt atcagcgaca aataatgacg tttacgccga taaaaagaca tttaataccc
     2341 tcacgatcaa cgtcactgat gagagtgata atcccctgac aaatcatcag gtcaccttta
     2401 agaatgaaaa aggaagcgcg gagtttgtcg aaccgccgca gcaaaatacg gatgcatatg
     2461 gtgttgccac aataaacatg gtaagtcagg ttgcggaaga aaatacgatt agcgccacgc
     2521 tgccaaatgg tttttcacaa cggataattg cgaaattcgt tagcgattcg agtacgccaa
     2581 aattcaaaca actggttgcc gatccagata ccattattgc tggcaacagc cagggcagta
     2641 ctctgaccgc catcatcaca gactttcata acaacccgtt aaaagatatg aaagtgaatt
     2701 ttgtggcacc tggtggctcg caactggaca acacgaccgc cacaacagac cagtccggta
     2761 ttgtgcgggt gcacctgacc agttcaaaag ctggtagcta ttccgtcgat gcctcgcttg
     2821 aggtggataa aaatattcac cagtcggtca cgatcaccgt ggtcccaaac agggaacaat
     2881 cggtaatgac cttgaatgcc gggtcgggca gtgcgatcgc taacaataca aatatcgtta
     2941 ccctgactgc cagtgtgaaa gatgtttatg gacacccgtt gccggatgag gatgtgaaat
     3001 ttaccttgcc agcctccatg accgggaact tcacgctaag tagtgaaacc gcccgcaccg
     3061 atgcaaacgg tgatgccgtg gtcacattgc gaggcacaaa agcgggtgag tttacagtta
     3121 cggcgacgct gaccagaaat aataccgttg cttatcagca agtcactttt attggggata
     3181 caaacagtgc gcagctccag ccgctgactg cctcattaaa ttccattgtt gcgggtaaca
     3241 gtacggggag taccctgacg gcaacgatcc tggacgctta ccaaaatccg cttaaagacc
     3301 agttggtcac tttccagagt aacgatgtca ctctaagcga aacagaagtc accaccaata
     3361 cgctgggtca ggcgacggta acaatgacca gcaatattgc cggacaacat aacgtcgtgg
     3421 tgagccggaa agcgcaagct tccgataata aaacgtttag tttatcagtg ctaccggatg
     3481 aaagttcggc gaaggtaata agtataaccg gagccgaaaa aacgataacg gtgggcgaaa
     3541 acatcacgct acggatactc gtccaggacg cgtttaacaa tgtaatcgcg ggtcaacgcg
     3601 tcagattaag tgcgcagcca acaactaaca ttacgatagg cgatacggct tacaccgata
     3661 ataacggtta tgcgtacgtt aaccttctca gcacccaacc tggggtttat caggtgacgg
     3721 caacgctgga caataacagt agtagtaagg ttgacgtgaa tgtggcaaat ggcaaactcg
     3781 agttaacatc atcgaaacca gaaactacgg tccataatag tgagggtatt acgctgaccg
     3841 caacggcgag aaatgcgcgg ggtgaattga tgccagggca aattatcacc tttagcgtaa
     3901 cgcctgaagg tgcaacgcta agcaatacag gggaagtcct tactgaccag tcaggtcagg
     3961 ccaaagtgac gctgaccagt gacaaagtga atgtctatac cgttacggcc ataatgggca
     4021 aagatgttcc cgttcagagc caggtaacgg ttgcggttaa ggcagatgct aaaacggcac
     4081 atgttgtgag cgtcgtggct tctcctgaca ccatcaccgc cgacggcatc gatagcagca
     4141 ccatcacttc acgagtagaa gatgattacg gattcccggt tgaaggtgtc gatattagtc
     4201 atggcttaga caccaaaggc agcccggtag ttaatattcc aactacgcgt accgatcagt
     4261 ccgggcaagt cacggcgaca ataaccagta cattggcaga aaccttaaca gtcaatgtgc
     4321 aagttcctgg cacagccaac caatccgcaa ccattacatt ggttgccggc acggccgatg
     4381 aaagtaagtc aattttgaaa tccgatgttg acactctgaa ggctgactac cagcagagcg
     4441 caaaacttac gctaacattg caagacaagt acggtaaccc gatagtgacg tctgatcatc
     4501 tggaatttgt ccagtcaggc cccttcgtga actttctcaa gttgagcgat attgattaca
     4561 gccaaagaaa ttatggcgag tacaccgtga ctgtcactgg cggaaaagag ggaacagcga
     4621 cactcattcc catgctgaac ggggttcatc aggcaaactt aagcatatcg ctgaatctca
     4681 tccaatcgat aaaagaaatg tccggtcatg tcactgcaaa caaccatacc ttctccacgg
     4741 ctaaattccc gagcgaaggc tttgcaggag cgtattacac actcaacaat gataactttg
     4801 aagcgggtaa aaccgttgat gattatatgt tttcaagttc acagggttgg gtgtctgtcg
     4861 atgcttcggg taaagtttct ttcgcaaata tcggcgatca aacgtcagtc acaataagcg
     4921 ctgttccccg acaaggaggt acaacctacc agaccttaat taagctgaaa ggctggtggg
     4981 tgaataatgg aaatcatacc aatatctggc tagctgccaa tgcgctctgt catgctaaaa
     5041 atgatggata taatcttcct ggcatcacac atttgacgtc tggcgaaaac aaacgcacgc
     5101 agggatcact gtatggtgaa tgggggaacg ttggagcgtt ttccagtaat tcgcaattta
     5161 caccgggagc ttactggaca agtgaatctg atgattacag tcggcactac tatgtgcaga
     5221 tgctaaccgg tatgaccgga agcgacgctg attccagccc ccaactgacc gcctgccgta
     5281 aatcacttta atttcagcag ttgttattaa aggataccct tttacgaggg tatcctttca
     5341 gactgataac aaacgctaca ttgccggatg tgctgcgctt atcaggccta tataacccct
     5401 gcaatatatt gaatttattg tttttgtacc ccagccggca taaacgacta ctcaagaaac
     5461 tcaacagcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa agggatgcct
     5521 cgtttcgcca tttcctgtgc tttgacggtt aagacaaccg gcgatccgtg tcttgctccc
     5581 actttgcgtg ccgttgcttc atcagcagag agatgcacgt aatgacgatc ccctgcaatc
     5641 agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta gagaaactgt
     5701 ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc ctgaaccgcg
     5761 cggatgcacc taccatcact ggaataacta aaacgctttt atcgctggtt gcgaccacgg
     5821 tatccagcaa tgcacgggtt aatcgcttgc ccgctttttg ggcacaaagt atgagcttat
     5881 cgatattcgc ccatccttca cggtccagta cgataccaat cgcctccggt ttgtgtcgga
     5941 ggacaaaact taaaaacttg ctcgtatccg ccagctcttt ttcgttgtac tttgccatcg
     6001 cgttttcgtc cctggatttt ctgtttttat ttaccaatgt cattgtcact gggtttatga
     6061 attttctgca actcaatcct gcgcagaacc ttttccacac agcgtaattt ttcccctatg
     6121 caaaaaacgc atagccagaa acatacttgc attttcccca tacccacatg gctgataagg
     6181 tttttaagtt ccaggaattt tcctggctca agcaacacac aaacacaaaa aaacaatggg
     6241 gattgttatg acacaacaag gggatgctgt agccggggag ctggcgacag aaaaagtggg
     6301 cattaaaggg tatctggctt tttttctcac cataatcttt ttttccggtg ttttttccgg
     6361 aacagacagc tggtggcgcg tttttgattt ttccgttctg aacggatcgt ttgggcaact
     6421 gcctggtgcg aatggagcca ccacctcatt tcgcggcgcg ggtggcgcgg gggctaaaga
     6481 tggctttctc tttgcgctgg agctggcacc gtcagtcatt ctctctctgg ggattatttc
     6541 aattaccgat ggtcttggtg gattacgcgc cgcgcagcag ttaatgacac ctgtgctaaa
     6601 accgttgttg ggcattccag ggatctgctc gctggcgctg atcgctaact tgcaaaacac
     6661 tgatgccgcc gccggtatga ccaaagagct ggcgcaggaa ggtgaaatta ccgaacgcga
     6721 taaagtgatt ttcgccgcat atcaaaccag cggtagcgcc atcatcacca actacttctc
     6781 ttccggcgtc gcggtgttcg cctttctggg cacgtccgta atcgtacctt tagccgtcat
     6841 cctggtgttt aaatttgtcg gagccaacat tttgcgcgtc tggcttaact tcgaagaacg
     6901 tcgcaaccca acgcaaggag cgcaagcatg acaactcagg tccgcaaaaa cgtcatggat
     6961 atgtttatcg atggcgcgcg ccgtggcttt actatcgcca cgaccaacct gctgccaaac
     7021 gtggtgatgg cgtttgtcat cattcaggcg ctgaaaatta ccggcctgct cgactgggtg
     7081 gggcatatct gtgaacccgt aatggcgctg tggggattac ccggtgaagc ggcaaccgtc
     7141 cttttagccg cattaatgag tatgggcggc gcagtcggtg ttgccgccag tctggcaacc
     7201 gctggcgcat taaccggtca cgatgtcacc gttttactgc ccgcgatgta tttaatgggc
     7261 aacccggtgc aaaatgtcgg tcgctgcctg gggacagccg aagtgaatgc caaatattac
     7321 ccgcatatca ttacggtctg cgtgattaac gcattgctgt cgatctgggt tatgcagctt
     7381 attgtttaac aaggagttac catgattgat tataccgcag ccggttttac cctgctgcag
     7441 ggagcgcatt tgtatgcgcc ggaagatcgg ggaatttgcg atgtcctcgt cgctaacggc
     7501 aaaattatcg ccgttgccag taatatccct tctgacattg taccggactg cacggttgtc
     7561 gatctcagcg ggcagatcct ctgcccaggt tttattgatc aacacgtcca tttgattggc
     7621 ggtggcggcg aagcaggtcc cacgacgcgc acgccggaag tggcgctaag tcgcctgacg
     7681 gaagcgggcg tcacgtcagt ggttggtctg ctgggcaccg actctatctc tcgccacccg
     7741 gaatccctgc tcgccaagac ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg
     7801 accggcgctt atcatgtccc ttcccgcacc attacgggtt ccgtggaaaa agacgtggcg
     7861 attatcgatc gtgtgattgg cgtgaaatgt gccatctctg atcaccgttc tgccgcaccg
     7921 gacgtttatc acctggctaa tatggcggca gaatcccgcg ttggcggttt gctcggcggt
     7981 aaacctggcg tcaccgtgtt ccacatgggc gacagtaaaa aggcattaca gcctatttat
     8041 gacctgctgg aaaactgcga tgtgccgatc agcaagctgc tgccgaccca cgttaaccgc
     8101 aacgtaccgt tgtttgagca ggcgctggag ttcgcgcgca aaggcggcac catcgatatc
     8161 accagcagca ttgacgaacc ggtcgcccct gccgaaggta ttgtccgcgc cgttcaggcg
     8221 ggtattccgc tggcacgcgt caccctcagc tccgacggca acggtagcca gccgttcttc
     8281 gatgatgaag ggaatttaac ccatatcggc gttgcaggtt ttgaaacgtt gctggaaacc
     8341 gtgcaggtgc tggttaaaga ctatgatttc agtatcagcg atgccctgcg cccgctcacc
     8401 agtagcgtag ccggtttcct taacctgacc gggaaaggcg aaattctgcc aggcaatgac
     8461 gcagacttac tggtcatgac gccagaattg cgcattgagc aggtatacgc tcgcggcaaa
     8521 ctgatggtca aagacggcaa agcctgcgtg aaaggaacgt ttgaaacggc ttaaacggtt
     8581 gtcagccgcc ctaatggacg tagaatgccc ccaagggcgg ctgacagagt aaaacgtaat
     8641 ggatgactgt ggtgcggttt tgcataatat tgaaaccaaa tggctttaag attttctgac
     8701 cctggaaaaa tgccgcaatt tttcccaggc ggcagtcagt cgtaacgtct cgcaaccggc
     8761 attcagccgc cgcatccgcg cgctggaaca ggcgattggc gttgaattgt ttaaccgcca
     8821 ggtgacgccg ctgcaactct cagaacaagg gaaaatcttc cattcgcaga tccgccatct
     8881 gttgcaacag ttagaaagca accttgcaga gctgcgtggc ggcagcgatt acgcgcaacg
     8941 taaaatcaag atagccgctg cacactctct ttccctcggt ctgttaccgt ccattatcag
     9001 ccagatgccg ccgctcttta cctgggcgat tgaagccatt gatgtcgatg aagcggtcga
     9061 taaactgcgt gaaggacaaa gtgactgtat tttttccttt cacgacgaag atttgctgga
     9121 agcgccgttt gaccacattc gcttatttga atctcaattg ttccctgtct gcgccagtga
     9181 cgaacatgga gaagcacttt ttgacctcgt gcagccacac tttccgttac tgaattacag
     9241 ccgcaactcc tacatggggc gattgattaa tcgcaccctg acgcgccaca gtgagttaag
     9301 tttcagcacc ttttttgtct cttcgatgag cgagctttta aagcaggttg ccctcgacgg
     9361 ctgtgggatt gcctggctgc cggagtatgc catccaacaa gaaattcgca gcgggcagct
     9421 cgttgtgctg aatcgggacg aactggtgat cccgattcag gcctacgcat accggatgaa
     9481 tacccgcatg aatccagttg ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattgt
     9541 gcttagctga cattctcaag cgtcgcactg cgccaaccgc cattatccca gaccagatca
     9601 aacgttagcg gttcatcgtc gtggtacagc attatctcaa ggctgatcgc cagagtgtgg
     9661 cagtacgctt ctttcaaatt aacctgcaac gccgcaatcc gtggctcatg aaataacagt
     9721 gcagaacgca gctgacggca ataccagctc tggagatgtt tgtcgctcgg gttatgcact
     9781 tgccactcgg gcatgcctga aacaagtaat gacggtaatg tcgaatgagg agaattaaaa
     9841 agagtgagga gatcgctgca taccgattta cgcagcaatg acgttttatt gcctgaaatt
     9901 tcaggtaaaa cagcctgaag tgattggcgc atcattttgc gcactcctga cgtttagcaa
     9961 catatgcggc ttgccctgaa caccgggcac cactgtattg atgctcattt tccatagcca
    10021 ttcccttttg ttcattacat ccatgtcagt ctgagacagg cctgaatcct gtcagcaaag
    10081 tataaaacct tacttgccta tgaatatcta ctgacttttc aggaaaaaag tcatgatttg
    10141 ataaaaatca tcaaactcaa aaaaaaaccg ctagtatatt ctgaatattc cgaatagtta
    10201 cgtagatgaa aatacttgtc accgctgcta cttaattccg gtgggaaatt atttatttcc
    10261 gcataatcag ccatgtatta tttgaaacaa ttagtttgta aataatataa attgataaca
    10321 tatcaaatac atcaaaaaca ccattcacag gcattattaa tatagacctc aatatagcaa
    10381 catcaaattc caattagtaa taagatatga ttttaaattg catgcaaata a
//
LOCUS       DAZKFZ010000163        10357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000163 DAZKFZ010000000
VERSION     DAZKFZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            43..672
                     /locus_tag="ACHOQW_003588"
     CDS             43..672
                     /locus_tag="ACHOQW_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9574603.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     CDDVNVAVIDIAMPDENGLSLVKRLRAQKPQFRAIILSIYDTPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGMGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALNHHLLAGH"
     gene            672..2213
                     /locus_tag="ACHOQW_003589"
     CDS             672..2213
                     /locus_tag="ACHOQW_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9574604.1"
                     /translation="MSRNLRHWVISLFIVLAWGSGWLMLWTLSFYLTNNGQQAVLFLP
                     HGVYLAFLILHSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLLPAIIAQNV
                     WHRFPLYWQRLTLLLASVTAASLLNTAMLTPFMSGSIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLRLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKSLARELHDEIGQNITAI
                     QIQSQLVTRARDPAQVHSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFTYQLTTTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEILHLEVSDNGIGIASGKMTGFGIQGMRERVSALGGDLTLEKHNGTRVIVNLPTFL
                     QQIAG"
     gene            2286..3602
                     /gene="uhpC"
                     /locus_tag="ACHOQW_003590"
     CDS             2286..3602
                     /gene="uhpC"
                     /locus_tag="ACHOQW_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9574605.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGIGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKSTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            3599..4630
                     /locus_tag="ACHOQW_003591"
     CDS             3599..4630
                     /locus_tag="ACHOQW_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9574606.1"
                     /translation="MKMPLTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCETET
                     KEFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTERLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDNYGSTDVRKALINK
                     WVSEVKMGK"
     gene            4699..6777
                     /locus_tag="ACHOQW_003592"
     CDS             4699..6777
                     /locus_tag="ACHOQW_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9574607.1"
                     /translation="MSQTLALHPAKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYSWSHFNISWLWYLLPSLLLVRPFNEAKREQRSRHYFDAGWALLCIAFMVIS
                     ATIVGRGLGYAAIVLFIELSAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFANDAGEFAPLAFMNVLSQAHIIQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAVIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFSGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVCSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            6789..7835
                     /gene="fbpC"
                     /locus_tag="ACHOQW_003593"
     CDS             6789..7835
                     /gene="fbpC"
                     /locus_tag="ACHOQW_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFP9574608.1"
                     /translation="MSQKNFVELRNVTKRFGSNMVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRNEVKQRVKDALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQELYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAYFPVQGRGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDTGEHYYL
                     EIHPYGMFVLADSA"
     gene            8093..8331
                     /locus_tag="ACHOQW_003594"
                     /pseudo
     CDS             8093..8331
                     /locus_tag="ACHOQW_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(8521..8826)
                     /locus_tag="ACHOQW_003595"
     CDS             complement(8521..8826)
                     /locus_tag="ACHOQW_003595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574609.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVFIVATCVIIQVSLERPV"
     gene            9146..9340
                     /locus_tag="ACHOQW_003596"
     CDS             9146..9340
                     /locus_tag="ACHOQW_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574610.1"
                     /translation="MNENRIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRPDKKHFLECNEDK"
     gene            9701..9829
                     /locus_tag="ACHOQW_003597"
     CDS             9701..9829
                     /locus_tag="ACHOQW_003597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574611.1"
                     /translation="MGADITEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(10140..>10357)
                     /locus_tag="ACHOQW_003598"
     CDS             complement(10140..>10357)
                     /locus_tag="ACHOQW_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574612.1"
                     /translation="NVEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSY
                     AFSIVTTNRNKNLAGANIKRIKRCDTV"
BASE COUNT         2458 a         2618 c         2627 g         2654 t
ORIGIN      
        1 gtgatgcatg ataggagaat gagataataa gagaagaaaa gtatgattcg ggttgtgctg
       61 gtggatgacc atgtggtggt gcgctctggt ttcgcacagt tactctctct ggaagacgat
      121 ctggaggtca ttggtcagta cagtagtgcc gcacaggcat ggtcggcatt aatctgtgat
      181 gacgtcaatg ttgccgtcat tgatattgct atgccggacg aaaacggcct cagtttggtt
      241 aagcgcctgc gtgcacaaaa accgcaattc cgcgccatta ttcttagcat ttatgacacg
      301 ccaacctttg tacaaagcgc tctggatgct ggtgccagtg gctatttgac caaacgctgt
      361 gggccggaag agttagtgca ggcggtgcgc tctgtcggta tgggagggca ttatctctgc
      421 gccgatgcaa ttcgggcttt acgcggcggc gggcaacccg cccaggcact ggaaattctc
      481 accccacgag aacgtgaagt gtttgattta ttagtcaaag gcgacagtgt aaaagagatt
      541 gcctttaagc tcgaactcag ccataaaacg gtacatgtac atcgcgccaa cgtgctggga
      601 aaactgaact gtcatagcac cattgagctg gtgcattttg cactcaacca ccatctgctg
      661 gcggggcatt aatgtcgcgt aaccttcgtc attgggtgat ttcgctgttt attgtgctgg
      721 cctggggaag cggctggttg atgttgtgga cgctcagctt ttatctgacc aataacggtc
      781 aacaggcagt gttatttctg ccgcacggag tttacctcgc ttttcttatt ttgcactctc
      841 gccgttactg gcctgcgttg gtgctccccc ctttgctgat gatgttctgg ctgcacagcg
      901 agcaactgtt gaacggctat ctcatgctgg caacgccggt tattagcctg cttcctgcca
      961 tcattgcaca aaacgtctgg caccgttttc cactttactg gcagcgtctg acgttgctgt
     1021 tagcctcagt aacagcagct tcattgctga atacagccat gctaacccct tttatgtcag
     1081 gctcaataat gctgcccggt ctgacctcat ttaccggcgg cgtattgttg acgccgtttg
     1141 tctatcttat ctttgagttt ctgcgccagc agcatcgcta tcagcttctg agactcgata
     1201 cgcataatcc ccccttgcga acctctttaa tcatctggtg tagcctattt tttattattg
     1261 gtatcggcac ccagatggtg ttgtcgcctg aaatcgaacg cctgctgctg atcgtggtat
     1321 ttctacccaa tgtagtcatg gcctggaaat ttggctggca agggggcgta ctctcggggc
     1381 ttttgggcag catgatgatc actattgccc gccagattgg tgtgggcttt agtaacctgg
     1441 tggaactgga gatttttctc gcaacccagg cattgctggg aattggcctg ggcattgcca
     1501 ttagtcggca gcaacatctg gcgctaaacc tgcatcatta ccgtcagcga ctggaggcag
     1561 aactggcagc acggcgggca ttggctgaaa agctgatcca taccgaggaa gacacgcgta
     1621 aaagcctggc tcgcgagctg cacgacgaaa tcggtcagaa cattaccgcc attcagatcc
     1681 agtcgcaact ggtgacgcgg gcacgcgatc cggcgcaagt tcattccgca gccagccaaa
     1741 tcaacgaact cgcccgccgc attcatctct ccacccgcca gttgctgcgc cagttgcggc
     1801 cccccgcgct tgatgaactg acattccgcg aagcattact tcatctgatt aatgaatttg
     1861 cctttagtga acgcggcatt cattgccagt ttacctatca actaaccacc acgccagaaa
     1921 acgaaacggt tcgctttaca ctgtatcggt tattgcagga gttactcaat aacgtctgta
     1981 aacacgctga agccagcgaa gtgacgatta ttctgcgcca gcagggagag atattgcacc
     2041 tggaggtcag cgacaacggt ataggaatcg ccagcgggaa aatgaccgga tttggcattc
     2101 aggggatgcg tgaacgcgtc agtgccctgg gcggcgactt aacgctcgaa aaacataacg
     2161 gtacgcgggt aattgttaac ctgcccacat ttttgcaaca aatcgccgga taaccaggaa
     2221 aaagtcttag tcatcccgga ttttctctca tcctctttca ctttcatttt catatagtca
     2281 gcaacatgga gacgaccatg cacgcgcgtt cagcgagaga gattaatcaa tgctaccgga
     2341 cattacgtcc acgattgctg atgtgcatga ttattggcta cgccgcattt tatctgacgc
     2401 gaaaaagcgt gaattacgtc cttccggcgc tgcaaaccga cctcggactg gataaaggcg
     2461 acattggcct gctgggttca ctgttttatc tcagctacgg attgtcgaaa tttaccgccg
     2521 gactgtggca tgacagccac ggacaacgcg gttttatggg tatcggcttg ttcgccactg
     2581 ggctattgaa cgtggtgttt gcctttggcg aatcacttac gcttctgctt gtggtctgga
     2641 cactgaacgg attttttcaa ggctgggggt ggccaccgtg tgcccgctta ctgacccatt
     2701 ggtattcccg taatgaacgt ggtttctggt ggggctgctg gaatatgtcg atcaacattg
     2761 gcggagcgat tattccgctg attagcgcct ttgccgccca ctggtggggc tggcaggccg
     2821 cgatgctgac accggggata atcagcatgg cattagggat ctggttaacg ctacaactca
     2881 aaagcacgcc ccaggaagag ggattaccga cagttggtca ctggcgtcac gatccgctgg
     2941 agttacgtca ggaacaacaa agtccgccga tggggctgtg gcaaatgttg cgcaccacca
     3001 tgctgcaaaa tccgctgatc tggctactgg gtgtctctta tgtacttgtt tatgtgatcc
     3061 ggattgcgct aaacgactgg ggcaatatct ggctgacgga aagccacggc gtcaacctgc
     3121 tcagcgccaa cgccacggta atgctgtttg aagtaggcgg cttactcggc gcgttatttg
     3181 ccggatgggg ttcggatttg ctgtttagcg ggcagcgagc ccccatgatt ttgctgttta
     3241 cccttgggtt gatggtttcg gtcgctgcac tctggttggc accggttcat cactatgccc
     3301 ttctggctgt gtgctttttt actgtgggct tttttgtctt tggcccgcaa atgctgattg
     3361 gacttgcggc agtggaatgt ggacacaaag ctgcggcggg ttcaatcacc ggatttctcg
     3421 gcctgttcgc ctatctcggt gcggcgctgg caggctggcc gttgtcgctg gttatcgaac
     3481 gctacggctg gtcgggaatg ttcagtttac tctcggtcgc cgcagtactt atgggtttac
     3541 tgctgatgcc gttactgatg gcgggcatca ccaccactca cgctcgaagg ataaaacaat
     3601 gaaaatgccc cttacctcta ctctgattgc ttctgccgtt gcgctggcaa ccttaacggg
     3661 tgccgcgcag gcaaaaggcc ggctggtggt ctattgcagc gccactaatg aaatgtgtga
     3721 gaccgaaacc aaagagtttg gcgagaaata tgatgtgaaa acctcgttta tccgcaacgg
     3781 ctccggtagc accctggcaa aagtcgatgc tgaaaagaaa aacccacagg ctgacgtctg
     3841 gtacggcggc actttagacc cgcagtcgca ggccggcgag atgggcctgt tacaggcata
     3901 taaatcgcct aaccttgaac agatcatgga aaaattccgc gatccggcaa aagtgaaagg
     3961 aaacctttcc tccgccgttt atgtggggat cctcgggttt ggcgtcaaca cagagcgtct
     4021 gaaagagaaa aacctgcctg tgccgaagtg ctggaaagat ctcaccaaac ctgaatataa
     4081 aggcgaaatt cagattgccg atccacaaag ttccggtacc gcctacaccg cgctggctac
     4141 ctttgcgcaa ctgtgggggg aagatcaggc ctttgattat ctaaaacaac ttaacgccaa
     4201 cgtctcccag tacaccaaat caggcattgc cccggcacgt aacgccgccc gcggcgaaac
     4261 ggcgattggt attggtttcc tgcatgacta ctcgctggaa aaagaacagg gcgcaccact
     4321 ggagttgatt tccccgtgtg aaggtacggg ctacgaaata ggcggcgtca gcattctgaa
     4381 aggtgcgcgc aacctcgaca acgccaaact gttcgtggac tgggtattat caaaagaagc
     4441 tcaggaactg gcgtggaaga aagggaaatc ttatcagatc ctgaccaata ccaccgccga
     4501 aacgtcgcca aattcgctga agctcgacga cctgaaatta atcaactacg acatggataa
     4561 ctacggttca actgatgttc gtaaggcatt aattaataaa tgggtcagcg aagtgaagat
     4621 gggtaaataa acccgtattg tgtgcacttg ccgggtgcag cacagacgcc tgccccggcc
     4681 aaccataccg gaactgctat gtcacagaca cttgcactcc atcccgcgaa aaagcgggat
     4741 gcgattttcc tttgggtttt gctgggctgg ctggcattta tcctgttgcc gagctggagc
     4801 ctcgattatg gtctgctgga gtccacgagt gatgagatcc tcgaggcgta cagctggtcg
     4861 cattttaata tcagctggct gtggtattta ctcccgagtt tgctgctggt tcgcccgttt
     4921 aatgaggcca aacgcgagca gcgtagccgt cattattttg atgccggctg ggcattgctt
     4981 tgtatagcgt ttatggtcat cagtgccacc atcgttggcc gcggtctagg ctacgccgcc
     5041 atcgtgctgt ttatcgaatt aagcgcgata atgacgctgg ccttaacccg cctcgaatgg
     5101 ctgggtggcg accgctttgt gatcggctcg ctgattgcga ttgtggcgct gattggcatt
     5161 tttatcgtct ggccgagcat cgccattttt atcccgatgt tcgccaacga cgcgggagag
     5221 ttcgccccac tggcgtttat gaatgtgctc tcgcaggccc atatcattca ggtgatcgtc
     5281 aactctatcg tcttatcgat agcggtgggt attggctgca ctttcttcgg tctggtgctg
     5341 gcgatatata ccacgcgcat cgctaaacgc agcgccgtaa tcggccgcat cttttccatt
     5401 ctgccaattg tcacgccacc gtttgtggtc ggcttaggcg ttacgctcat gatggggcgc
     5461 tccggctaca taacggaatt tatggtggag tggtttgggc tgaccaacac caactggctg
     5521 tatggtttta ccgggatctg gctggcacag gtcctggcat ttacgccaat ggcttttatg
     5581 atcctcgatg gggccattaa aaccattcat ccctcgctgg aagaagcctc ctacaccctg
     5641 cgcgccagcc gctggcaaac ctttagcggt gtgtttgtgc cgttgttaaa gcccgcgctt
     5701 gccaatgcct ttttaatcgt cgtggtgcag tcactggctg acttcagtaa cccgctggtg
     5761 cttggcggca acttcgacgt actggcgacg caaatttact tctacattac cggctcgcag
     5821 ctggattatc aggccgccag cacccttggc gctttcctgc tgctgttctc gctactggtg
     5881 ttctgcgtcc agtatatgtg gattggtaaa cgctcttacg tcacggtttc cggaaagtcc
     5941 taccgcggcg atgtccagcc actaccggtc accctggtgt gtagcgtggt cgccatactt
     6001 gcgatatgga ttgcctttaa cgccctgctc tatggcagca ttttctacgg cagcttcacc
     6061 gtgaactggg gcgtggacta caccctaacg ctggataact ttatcaagtt atttggtcag
     6121 gggatgagcg acggcgcgtg gccttcactg ttagatacct tgctctacgc cgggattgcc
     6181 gcacccatta ccgctatttt tggcctgctc atcgcctggg tggtggtgcg ccaacaattc
     6241 aaaggcaaaa agaccatcga gttcaccacc atgttgtgct ttgccgtacc gggtacagtg
     6301 gcgggtgtct cgtatattct tgcctttaat agcgccccag tctatctcac cggaacggca
     6361 gcaattgtga ttatctcgat ggtgatgcgt aacgttccgg tgggtattcg cgccgggatt
     6421 gcaggtctgg ggcagatcga caaatcgctg gatgaagcct cacttagcct gcgtgcaggt
     6481 tccctgcgaa ccattacgca cattttactg ccgttattac gcccagctat tctctcggcc
     6541 ctgatttaca gctttgtgcg ggctatcacg acggtcagcg ccatcgtctt tctcgtcacg
     6601 ccggacaccc gcgtggcaac cgcctacatc cttaaccgcg tagaagacgg cgaatatggc
     6661 gtagccatcg cctacggttc gattctgatc gtggtgatgc tggcgattat tttcctcttt
     6721 gactggctga tcggtgaagc acgtatttcc cgttctaaag ccaaaaacca ggcgtaatgg
     6781 agcgcactat gagtcagaaa aattttgtgg aactgcgcaa cgtcactaaa cgattcggca
     6841 gtaatatggt tatcgacaat atcaatctca ccatcccaca agggcaaatg gtgacgctgc
     6901 tcggtccttc cggatgcggc aaaaccacca ttctgcgtct ggttgccggg ctggaaaaac
     6961 cgagcgaagg gcaaattttt atagatggcg aagatgtcac gcatcgttcc attcagcaac
     7021 gcgatatctg catggtgttt cagtcatacg ctctgttccc acatatgtcg ctgggcgaaa
     7081 acgttggcta tgggctgaaa atgcttggcg tgtcgcgtaa tgaagtgaag cagcgggtga
     7141 aagatgcgct ggcaatggtg gatctggaag gattcgaaga ccgctatgtc gatcagattt
     7201 ccggcggtca gcaacagcgt gtggctctgg cccgtgcgtt gatcctcaag ccaaaggtgc
     7261 tgctgtttga tgagccgtta agtaacctcg atgccaacct gcgccgcagt atgcgcgata
     7321 agatccgcga gctgcaaaag cagtttaata tcacgtcgct ctacgtgact cacgatcaaa
     7381 gtgaagcttt cgcggtgtcc gacactgtac tggtgatgaa taaaggtcac atcatgcaga
     7441 tcggttcacc gcaggagctc tatcgtcagc ccgcctcccg ctttatggca agttttatgg
     7501 gcgacgccaa cctgttcccg gcgaacttta gcgaagagta tgtcgatatc tacggttatc
     7561 gcctgccgcg cgcggcgtat ttcccggtgc aaggtagagg caccgtcggc gttcgcccgg
     7621 aagccatcac cttaagcaat cacggcgaag agagtcagcg ttgtgttatt cgccatgtcg
     7681 cttacatggg gccgcagtac gaagtgaccg tagaatggca tggtcaggag attttattgc
     7741 aagtcaacgc cactcgttta cagcccgata ctggtgagca ctattacctc gaaatccatc
     7801 cttacgggat gtttgtttta gcggattcgg cataaaaata gcgcataaaa ggtcccctcc
     7861 agctcagctg tctcttatac acaaataacc agccggagat ttgtgatgtc tgtcagcctg
     7921 ctttaggggg gactctttcc atccggtcac ccgcagccat tttttgaact tttcttcacc
     7981 cgttgctctt ctcttatatt tcattttctt gtaagtgccc catcagcgac gtcttgtgaa
     8041 aattgtcctg tctggcaaca atcgcgccca tctatattga tgggctcgaa cgatgaactc
     8101 tcaaacaaca ctagatatca ccggctttcg cacttatttt ccagacgcat tacgtctcag
     8161 atttgacgag caattatctg tccgggcaat cgcacagcag ttaagactca gttattcccc
     8221 cattcacaat ctctttcaac gaattaacgc ctcaggtatt gtctgactat gacccgagtc
     8281 cctttcattc gcccaaccga taccatactt tatgccaaca gaaagaatga atgaaccgcg
     8341 cctaaaatca gtgaagacac atggcacaaa gcgcgacatg tctcacattt aacccctgag
     8401 gggggcaaca agtataaaac cgttttttga ttaagtggtc ggatatgaaa aagggaaggt
     8461 ttatgtgaaa aatacctgtg ttttctttga aaacacacaa cagtatcagg ttatttatgt
     8521 ttatacaggg cgttccaaac tgacctgaat aattacgcaa gttgcaacaa taaaaacatt
     8581 tttgccgtag tgtgaggtac tccggtaaat tgctttccag gtgcagacaa tgactaacga
     8641 agaactacag gcaatcgcat tatccatgct tgagcgccag cgcgaaaaag tacaccagca
     8701 acgagagtct ttgcttaacc catctccatt caccaggcca aaacgattgg agcttgaagc
     8761 attcttggac aaatacccac ggcggcttaa atcaggcaaa accagaccac caggctgata
     8821 gctcacagtt tgttctccta ttcaataaag cggataagaa ccatcagcgc ggtagcgtaa
     8881 ggcattactg cgcattgctt tacgagtaat aacaatggat ttatcaccag aagacgcaaa
     8941 gtttttacga gaactgcaac accgtatgct ctgagaagcc attatcgaac agcaaacggg
     9001 catgagcgcc atagacccat tgcgataaaa acgggcctca tctcctttag ttgacgacaa
     9061 ttccaatgcg gataaaacac gtccaccaca ctgattacat ttttcattag cacagtggtg
     9121 taatgcagca tacgaggaaa accggatgaa tgagaacaga ataaaaaggc tggagcaact
     9181 gttacaggca cgccaacggg agtttgccac taaatgggga cgtgccgcgt tgccacatga
     9241 acgcctaacc tattttccac ttgaagacct cattaaaaat aatggcaaaa aaatcaggcc
     9301 tgataagaaa cactttttag aatgcaatga agacaaataa caacaatctg catattgcca
     9361 ccaaccacag atgtggcatc gttatcagcg cgcttgattt tctttatatc cactgacgag
     9421 cttaccgggg cacctttgtt tgccggtcat tctggtatca tgcagatatt tacggcgtac
     9481 aagcccacta gcatgacatt gtaataaggc ctgagcctaa cgctttaatg tgtgtcatac
     9541 actcagtggc gtaatcgttg acaatatggt gcatgtaatc gtaagtgtcc agcgaaatcc
     9601 atctggtcat aatctgaagc atccgacaaa gtggtgtcca ccaaataagt gatctgcgcc
     9661 caatatcctg agccggaact ttgacgaagg ccaacgaaaa atgggggcgg atattacaga
     9721 gttctcggta caggggaaaa gtggtacctg tcgccagttc tccgatctgt ttaaccggga
     9781 aattatctcc tacagcttgt cagaaaggtc ggtgatggag catcgttaat acgattctgt
     9841 acgatgcatt cacagtgccc ggccagagga tgccccgtcg ctgcatacgg atcagggttg
     9901 gcaatatcga atcgcaggct ataggcaaag ttaaaagccc catggagtag cacaaaatat
     9961 gccgcacaaa ggaaacggtc tgaataacgc agtgatggag aacttcttcg gcacacagct
    10021 aaagcgcata gtgatcgagc gctgattctg gcgaacaact gaactaacac atcagaatct
    10081 gcattatgtt aaataaatat gaaaagatgg tttaaatacc ccgttacttt tgacttacac
    10141 tatacggtat cgcatcgttt aatacgttta atattcgcac cggccagatt tttatttcta
    10201 ttagttgtca caatactgaa tgcgtacgac cacagtattc tggctcctgt gtggttatgc
    10261 tttaattctg cgttccgggc agataagcag ttgcttgcag gaatccttct tgtgttaatg
    10321 tcagttcccc ttttaccagt gctgatttcc acattcc
//
LOCUS       DAZKFZ010000164        10343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000164 DAZKFZ010000000
VERSION     DAZKFZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            270..674
                     /gene="sdhC"
                     /locus_tag="ACHOQW_003599"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             270..674
                     /gene="sdhC"
                     /locus_tag="ACHOQW_003599"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9574613.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            668..1015
                     /gene="sdhD"
                     /locus_tag="ACHOQW_003600"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             668..1015
                     /gene="sdhD"
                     /locus_tag="ACHOQW_003600"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP9574614.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1015..2781
                     /gene="sdhA"
                     /locus_tag="ACHOQW_003601"
     CDS             1015..2781
                     /gene="sdhA"
                     /locus_tag="ACHOQW_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP9574615.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2797..3513
                     /gene="sdhB"
                     /locus_tag="ACHOQW_003602"
     CDS             2797..3513
                     /gene="sdhB"
                     /locus_tag="ACHOQW_003602"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP9574616.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3814..6615
                     /gene="sucA"
                     /locus_tag="ACHOQW_003603"
     CDS             3814..6615
                     /gene="sucA"
                     /locus_tag="ACHOQW_003603"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP9574617.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6630..7847
                     /gene="odhB"
                     /locus_tag="ACHOQW_003604"
     CDS             6630..7847
                     /gene="odhB"
                     /locus_tag="ACHOQW_003604"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP9574618.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7941..9107
                     /gene="sucC"
                     /locus_tag="ACHOQW_003605"
     CDS             7941..9107
                     /gene="sucC"
                     /locus_tag="ACHOQW_003605"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP9574619.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9107..9976
                     /gene="sucD"
                     /locus_tag="ACHOQW_003606"
     CDS             9107..9976
                     /gene="sucD"
                     /locus_tag="ACHOQW_003606"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP9574620.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         2435 a         2697 c         2900 g         2311 t
ORIGIN      
        1 aaacccgaca aactatatgt aggttaattg taatgatttt gtgaacggcc tatactgccg
       61 ccaggtctcc ggaacaccct gcaatcccga gccacccagc gttgtaacgt gtcgttttcg
      121 catctggaag cagtgttttg catgacgcgc agttatagaa aggacgctgt ctgacccgca
      181 agcagaccgg aggaaggaaa tcccgacgtc tccaggtaac agaaagttaa cctctgtgcc
      241 cgtagtcccc agggaataat aagaacagca tgtgggcgtt attcatgata agaaatgtga
      301 aaaaacaaag acctgttaat ctggacctac agaccatccg gttccccatc acggcgatag
      361 cgtccattct ccatcgcgtt tccggtgtga tcacctttgt tgcagtgggt atcctgctgt
      421 ggcttctggg taccagcctc tcttcccctg aaggtttcga gcaagcttcc gcgattatgg
      481 gcagcttctt cgtcaaattt atcatgtggg gcatccttac cgctctggcg tatcacgtcg
      541 tcgtaggtat tcgccacatg atgatggatt ttggctatct ggaagaaaca ttcgaagcgg
      601 gtaaacgctc cgccaaaatc tcctttgtta ttactgtcgt gctttcactt ctcgcaggag
      661 tcctcgtatg gtaagcaacg cctccgcatt aggacgcaat ggcgtacatg atttcatcct
      721 cgttcgcgct accgctatcg tcctgacgct ctacatcatt tatatggtcg gttttttcgc
      781 caccagtggc gagctgacat atgaagtctg gatcggtttc tgcgcctctg cgttcaccaa
      841 agtgttcacc ctgctggcgc tgttttctat cttgatccat gcctggatcg gcatgtggca
      901 ggtgttgacc gactacgtta aaccgctggc tttgcgcctg atgctgcaac tggtgattgt
      961 cgttgcactg gtggtttacg tgatttatgg attcgttgtg gtgtggggtg tgtgatgaaa
     1021 ttgccagtca gagaatttga tgcagttgtg attggtgccg gcggcgcagg tatgcgcgcg
     1081 gcgctgcaaa tttcccagag cggccagacc tgtgcgctgc tctctaaagt cttcccgacc
     1141 cgttcccata ccgtttctgc gcaaggcggc attaccgttg cgctgggtaa tacccatgaa
     1201 gataactggg aatggcatat gtacgacacc gtgaaagggt cggactatat cggtgaccag
     1261 gacgcgattg aatatatgtg taaaaccggg ccggaagcga ttctggaact cgaacacatg
     1321 ggcctgccgt tctcgcgtct cgatgatggt cgtatctatc aacgtccgtt tggcggtcag
     1381 tcgaaaaact tcggcggcga gcaggcggca cgcactgcgg cagcggctga ccgtaccggt
     1441 cacgcactgt tgcacacgct ttatcagcag aacctgaaaa accacaccac cattttctcc
     1501 gagtggtatg cgctggatct ggtgaaaaac caggatggcg cggtggtggg ttgtaccgca
     1561 ctgtgcatcg aaaccggtga agtggtttat tttaaagccc gcgctaccgt gctggcgact
     1621 ggcggagcag ggcgtattta tcagtctacc actaacgccc acattaacac cggcgacggc
     1681 gtcggcatgg ctatccgcgc aggcgtaccg gtgcaggata tggaaatgtg gcaattccac
     1741 ccgaccggca ttgccggtgc gggcgtactg gtcaccgaag gttgccgtgg tgaaggcggt
     1801 tatctgctga acaaacatgg cgaacgcttt atggagcgtt atgcgccgaa cgccaaagac
     1861 ctggcgggcc gtgatgtggt ggcgcgttca atcatgattg aaatccgtga aggtcgcggc
     1921 tgtgatggtc cgtgggggcc acacgcgaaa ctgaaactcg atcacctggg taaagaagtt
     1981 ctcgaatccc gtctgccggg tatcctggag ctttcccgta ccttcgctca cgttgatccg
     2041 gtgaaagagc cgattccggt tatcccaacc tgtcactaca tgatgggcgg tattccgacc
     2101 aaagttaccg gtcaggcgct gactgtgaat gagaaaggcg aagatgtggt tgttccgggg
     2161 ctgttcgcgg taggtgaaat tgcctgcgta tcggtacacg gtgctaaccg tctgggcggc
     2221 aactcgctgc tggacctggt ggtctttggt cgcgcagcgg gtctgcatct gcaagagtct
     2281 atcgccgagc agggcgcatt gcgcgatgcc agcgagtctg atgttgaagc atctttggat
     2341 cgtctgaacc gctggaacaa taatcgtaac ggtgaagatc cggtggcgat tcgtaaagcg
     2401 ctgcaagaat gtatgcagca taacttctcg gtcttccgtg aaggtgatgc gatggctaaa
     2461 gggcttgagc agttgaaagt gatccgcgag cgtctgaaaa atgcccgtct ggatgacact
     2521 tccagcgagt tcaacaccca gcgcgttgag tgcctggaac tggataacct gatggaaacg
     2581 gcgtatgcaa cggctgtttc tgccaacttc cgtaccgaaa gccgtggcgc acatagccgc
     2641 ttcgacttcc cggatcgtga tgatgaaaac tggctgtgcc actccctgta tctgccagag
     2701 tcggaatcca tgacgcgccg aagcgtcaac atggaaccga aactgcgccc ggcattcccg
     2761 ccgaagattc gtacttacta atgcggagac aggaaaatga gactcgagtt ttcaatttat
     2821 cgctataacc cggatgttga tgatgctccg cgtatgcagg attacaccct ggaagcggaa
     2881 gaaggtcgcg acatgatgct gctggatgcg cttatccagc tgaaagagaa agatcccagc
     2941 ctgtcgttcc gccgctcctg ccgtgaaggt gtgtgcggtt ccgacggtct gaacatgaac
     3001 ggcaagaatg gtctggcctg tattaccccg atttcggcac tcaaccagcc gggcaagaag
     3061 attgtgattc gcccgctgcc aggtttaccg gtgatccgcg atttggtggt agacatggga
     3121 caattctatg cgcaatatga gaaaattaag ccttacctgt tgaataatgg acaaaatccg
     3181 ccagctcgcg agcatttaca gatgccagag cagcgcgaaa aactcgacgg gctgtatgaa
     3241 tgtattctct gcgcatgttg ttcaacctct tgtccgtctt tctggtggaa tcccgataag
     3301 tttatcggcc cggcaggctt gttagcggca tatcgtttcc tgatcgatag ccgtgatacc
     3361 gagactgaca gccgcctcga cggtttgagc gatgcattca gtgtattccg ctgtcatagc
     3421 atcatgaact gcgtcagtgt atgtccgaag gggctgaacc cgacgcgcgc catcggccat
     3481 atcaagtcga tgttgttgca acgtaatgcg taaaccgtag gcctgataag acgcgcaagc
     3541 gtcgcatcag gcaaccagtg ccggatgcgg cgtgaacgcc ttatccggcc tacaagtcat
     3601 tacccgtagg cctgataagc gcagcgcatc aggcgtaaca aagaaatgca ggaaatcttt
     3661 aaaaactgcc cctgacacta agacagtttt taaaggttcc ttcgcgagcc actacgtaga
     3721 caagagctcg caagtgaacc ccggcacgca catcactgtg cgtggtagta tccacggcga
     3781 agtaagcata aaaaagatgc ttaagggatc acgatgcaga acagcgcttt gaaagcctgg
     3841 ttggactctt cttacctctc tggcgcaaac cagagctgga tagaacagct ctatgaagac
     3901 ttcttaaccg atcctgactc ggttgacgct aactggcgtt cgacgttcca gcagttacct
     3961 ggtacgggag tcaaaccgga tcaattccac tctcaaacgc gtgaatattt ccgccgcctg
     4021 gcgaaagacg cttcacgtta ctcttcaacg atctccgacc ctgacaccaa tgtgaagcag
     4081 gtaaaagtcc tgcagctcat taacgcatac cgcttccgtg gtcaccagca tgcgaatctc
     4141 gatccgctgg gactgtggca gcaagataaa gtggccgatc tggatccgtc tttccacgat
     4201 ctgaccgaag cagacttcca ggagaccttc aacgtcggtt catttgccag cggcaaagaa
     4261 accatgaaac tcggcgagct gctggaagcc ctcaagcaaa cctactgcgg cccgattggt
     4321 gccgagtata tgcacattac cagcaccgaa gaaaaacgct ggatccaaca gcgtatcgag
     4381 tctggtcgcg cgactttcaa tagcgaagag aaaaaacgct tcctaagcga actgaccgcc
     4441 gctgaaggcc ttgaacgtta cctcggtgca aaattccctg gcgcaaaacg cttctcgctg
     4501 gaaggcggtg acgcgttaat cccgatgctt aaagagatga ttcgccacgc tggcaacagc
     4561 ggcacccgcg aagtggttct cgggatggcg caccgtggtc gtctgaacgt gctggtgaac
     4621 gtgctgggta aaaaaccgca agacttgttc gacgagtttg ccggtaaaca taaagaacac
     4681 ctcggcacgg gtgacgtgaa ataccacatg ggcttctcgt ctgacttcca gaccgatggc
     4741 ggcctggtac atctggcgct ggcgtttaac ccgtctcacc ttgagattgt cagcccggtc
     4801 gttatcggtt ctgttcgtgc ccgtctggac agacttgatg agccgagcag caacaaagtg
     4861 ctgccaatca ccattcacgg tgacgccgca gtgaccgggc agggcgtggt tcaggaaact
     4921 ctgaacatgt cgaaagcgcg tggttatgaa gttggcggta cggtacgtat cgttatcaac
     4981 aaccaggttg gcttcaccac ctctaacccg ctggatgccc gttcaacgcc gtactgtact
     5041 gatatcggta agatggttca ggcaccgatt tttcacgtta acgcggatga tccggaagcc
     5101 gttgcctttg tgacccgtct ggcgctcgat ttccgtaaca cctttaaacg tgatgtcttc
     5161 atcgacctgg tgtgctaccg ccgtcacggc cacaacgaag ccgacgagcc gagcgcaacc
     5221 cagccgctga tgtatcagaa aatcaaaaaa catccgacgc cgcgcaaaat ctatgctgac
     5281 aagctggagc aggaaaaagt ggcgacgctg gaagatgcca ccgagatggt taacctgtac
     5341 cgcgatgcgc tggatgctgg cgattgcgta gtggcagagt ggcgtccgat gaacatgcac
     5401 tctttcacct ggtcgccgta cctcaaccac gaatgggacg aagagtaccc gaataaagtt
     5461 gagatgaagc gcctgcagga gctggcgaaa cgcatcagca cggtgccgga agcggttgaa
     5521 atgcagtctc gcgttgccaa aatttatggc gatcgccagg cgatggctgc cggtgagaaa
     5581 ctgttcgact ggggcggcgc ggaaaacctc gcttacgcca cgctggttga cgaaggcatt
     5641 ccggttcgcc tgtcgggtga agactccggt cgcggtacct tcttccaccg ccacgcggtg
     5701 atccacaacc agtctaacgg ttccacttac acgccgctgc aacacatcca taacggccag
     5761 ggcgcgttcc gtgtctggga ctccgtactt tctgaagaag cagtgctggc gtttgaatac
     5821 ggttatgcca ccgcagaacc acgcactctg actatctggg aagctcagtt cggtgacttc
     5881 gccaacggtg cgcaggtggt tatcgaccag ttcatctcct ctggcgaaca gaaatggggc
     5941 cggatgtgtg gtctggtgat gttgctgccg cacggttacg aagggcaggg gccggagcac
     6001 tcctccgcgc gtctggaacg ttatctgcaa ctttgtgctg agcaaaacat gcaggtgtgc
     6061 gtaccgtcta ccccggcaca ggtttaccac atgctgcgtc gtcaggcgct gcgcgggatg
     6121 cgtcgtccgc tggtcgtgat gtcgccgaaa tccctgctgc gtcatccgct ggcggtttcc
     6181 agcctcgaag aactggcgaa cggcaccttc ctgccagcca tcggtgaaat cgacgagctt
     6241 gatccgaagg gcgtgaagcg cgtagtgatg tgttctggta aggtttatta cgacctgctg
     6301 gaacagcgtc gtaagaacaa tcaacacgat gtcgccattg tgcgtatcga gcaactctac
     6361 ccgttcccgc ataaagcgat gcaggaagtg ttgcagcagt ttgctcacgt caaggatttt
     6421 gtctggtgcc aggaagagcc gctcaaccag ggcgcatggt actgcagcca gcatcatttc
     6481 cgtgaagtga ttccgtttgg ggcttctctg cgttatgcag gccgcccggc ctccgcctct
     6541 ccggcggtag ggtatatgtc cgttcaccag aaacagcaac aagatctggt taatgacgcg
     6601 ctgaacgtcg aataaataaa ggatacacaa tgagtagcgt agatattctg gtccctgacc
     6661 tgcctgaatc cgtagccgat gccaccgtcg caacctggca taaaaaaccc ggtgacgcag
     6721 tcgtacgtga tgaagtgctg gtagaaatcg aaactgacaa agtggtactg gaagtaccgg
     6781 catcagcaga cggcattctg gatgcggttc tggaagatga aggtacaacg gtaacgtctc
     6841 gtcagatcct tggtcgcctg cgtgaaggca acagcgccgg taaagaaacc agcgccaaat
     6901 ctgaagagaa agcgtccact ccggcgcaac gccagcaggc gtctctggaa gagcaaaaca
     6961 acgatgcgtt aagcccggcg atccgtcgcc tgctggctga acataacctc gacgccagcg
     7021 ccattaaagg caccggtgtg ggtggtcgtc tgacccgtga agatgtggaa aaacatctgg
     7081 cgaaagctcc ggcgaaagag tctgcaccgg cagcggctgc tccggcggcg caaccggctc
     7141 tggctgcacg tagcgaaaaa cgtgtcccga tgactcgcct gcgtaagcgt gtggcagagc
     7201 gtctgctgga agcgaaaaac tccaccgcca tgctgaccac gttcaacgaa gtcaacatga
     7261 agccgattat ggatctgcgt aagcagtacg gtgaagcgtt tgaaaaacgc cacggcatcc
     7321 gtctgggctt tatgtccttc tacgtgaaag cggtggttga agccctgaaa cgttacccgg
     7381 aagtgaatgc ttctatcgac ggcgatgacg tggtttacca caactatttc gacgtcagca
     7441 tggcggtttc tacgccgcgc ggcctggtga cgccggtact gcgtgatgtc gataccctcg
     7501 gcatggcaga catcgagaag aaaatcaaag agctggcagt taaaggccgt gacggcaagc
     7561 tgacggttga agatctgacc ggtggtaact tcaccatcac caacggtggt gtgttcggtt
     7621 ccctgatgtc tacgccgatc atcaacccgc cgcagagcgc aattctgggt atgcacgcta
     7681 tcaaagatcg tccgatggct gtgaatggtc aggttgagat cctgccgatg atgtacctgg
     7741 cgctgtccta cgatcaccgt ctgatcgatg gtcgcgaatc cgtgggcttc ctggtaacga
     7801 tcaaagagtt gctggaagat ccgacgcgtc tgctgctgga cgtgtagtag tttaagtttc
     7861 acctgcactg tagaccgacg tggatagggt gtcggtctac ggtttaaaag ataacgatta
     7921 ctgaaggatg gacagaacac atgaacttac atgaatatca ggcaaaacaa ctgtttgccc
     7981 gctatggctt accagcaccg gtgggttatg cctgtactac tccgcgcgaa gcagaagaag
     8041 ccgcttcaaa aatcggtgcc ggtccgtggg tagtgaaatg tcaggttcac gctggtggcc
     8101 gcggtaaagc gggcggtgtg aaagttgtaa acagcaaaga agacatccgt gcttttgcag
     8161 aaaactggct gggcaagcgt ctggtaacgt atcaaacaga tgccaatggc caaccggtta
     8221 accagattct ggttgaagca gcgaccgata tcgctaaaga gctgtatctc ggtgccgttg
     8281 tagaccgtag ttcacgtcgc gtggtcttta tggcctccac cgaaggcggc gtggaaatcg
     8341 aaaaagtggc ggaagaaact ccgcacctga tccacaaagt tgcgcttgat ccgctgactg
     8401 gcccgatgcc gtatcaggga cgcgagctgg cgttcaaact gggtctggaa ggtaaactgg
     8461 ttcagcagtt caccaaaatc ttcatgggcc tggcgaccat tttcctggag cgcgacctgg
     8521 cgctgatcga aatcaacccg ctggtcatca ccaaacaggg cgatctgatt tgcctcgacg
     8581 gcaaactggg cgctgatggc aacgccctgt tccgccagcc tgatctgcgc gaaatgcgtg
     8641 accagtcgca ggaagatccg cgtgaagcac aggctgcaca gtgggaactg aactacgttg
     8701 cgctggacgg taacatcggt tgtatggtta acggcgcagg cctggcgatg ggtacgatgg
     8761 acatcgttaa actgcacggc ggcgaaccgg ctaacttcct ggacgttggc ggcggcgcaa
     8821 ccaaagaacg tgtaaccgaa gcgttcaaaa tcatcctctc tgacgacaaa gtgaaagccg
     8881 ttctggttaa catcttcggc ggtatcgttc gttgcgacct gatcgctgac ggtatcatcg
     8941 gtgcggtagc ggaagtgggt gttaacgtac cggtcgtggt acgtctggaa ggtaacaacg
     9001 ccgaactcgg cgcgaagaaa ctggctgaca gcggcctgaa tattattgca gcaaaaggtc
     9061 tgacggatgc agctcagcag gttgttgccg cagtggaggg gaaataatgt ccattttaat
     9121 tgataaaaac accaaggtta tctgccaggg ctttaccggt agccagggga ctttccactc
     9181 agaacaggcc attgcatacg gcactaaaat ggttggcggc gtaaccccag gtaaaggcgg
     9241 tactacccac ctcggcctgc cggtgttcaa caccgtgcgt gaagctgttg ctgctactgg
     9301 cgctaccgct tctgttatct acgtaccagc accgttctgc aaagactcca ttctggaagc
     9361 catcgacgca ggcatcaaac tgattatcac catcactgaa ggcatcccga cgctggatat
     9421 gctgaccgtg aaagtgaagc tggatgaagc aggcgttcgt atgatcggcc cgaactgccc
     9481 aggcgttatc actccgggtg aatgcaaaat cggtattcag cctggtcaca ttcacaaacc
     9541 gggtaaagtg ggtatcgttt cccgttccgg tacactgacc tatgaagcgg ttaaacagac
     9601 cacggattac ggtttcggtc agtcgacctg tgtcggtatc ggcggtgacc cgatccctgg
     9661 ctctaacttt atcgacattc tcgaaatgtt cgaaaaagat ccgcagaccg aagcgatcgt
     9721 gatgatcggt gagatcggcg gtagcgctga agaagaagca gctgcgtaca tcaaagagca
     9781 cgttaccaag ccagttgtgg gttacatcgc tggtgtgact gcgccgaaag gcaaacgtat
     9841 gggccacgcg ggtgccatca ttgccggtgg gaaagggact gcggatgaga aattcgctgc
     9901 tctggaagcc gcaggcgtga aaaccgttcg cagcctggcg gatatcggtg aagcactgaa
     9961 aactgttctg aaataaatat ctgtaataag aaatagccct cgccgcttcc ctctacagga
    10021 atggcgaagg gctgtcggtt tcgacatggt tggccatcgt atgatggcct tttttatgcc
    10081 ttaaacggtt aaccacggtc gaaatctgac tgcaggcttg actgcatgac cacagccact
    10141 gtggattgcc gcaaccgacc gcaagccacg aataagaacg acggcgaatt tcgaatcgtt
    10201 acctgatgca ctggcttccc agcgtttgac cgtagcgtta agtaaatcaa caaaagcgat
    10261 gttcaccgct tttcgtcaag tcgtgccatt tgtcagccag gatcgggtct ttaccccgga
    10321 tattgcagca tcgcaaaaat tct
//
LOCUS       DAZKFZ010000165        10235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000165 DAZKFZ010000000
VERSION     DAZKFZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            93..986
                     /locus_tag="ACHOQW_003607"
     CDS             93..986
                     /locus_tag="ACHOQW_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574621.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            993..1907
                     /locus_tag="ACHOQW_003608"
     CDS             993..1907
                     /locus_tag="ACHOQW_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9574622.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2601..2819
                     /gene="yiiE"
                     /locus_tag="ACHOQW_003609"
     CDS             2601..2819
                     /gene="yiiE"
                     /locus_tag="ACHOQW_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9574623.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            3036..3278
                     /locus_tag="ACHOQW_003610"
     CDS             3036..3278
                     /locus_tag="ACHOQW_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9574624.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3608..4537)
                     /gene="fdhE"
                     /locus_tag="ACHOQW_003611"
     CDS             complement(3608..4537)
                     /gene="fdhE"
                     /locus_tag="ACHOQW_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP9574625.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4534..5169)
                     /gene="fdoI"
                     /locus_tag="ACHOQW_003612"
     CDS             complement(4534..5169)
                     /gene="fdoI"
                     /locus_tag="ACHOQW_003612"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9574626.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5166..6068)
                     /gene="fdoH"
                     /locus_tag="ACHOQW_003613"
     CDS             complement(5166..6068)
                     /gene="fdoH"
                     /locus_tag="ACHOQW_003613"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP9574627.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(6081..9131)
                     /gene="fdnG"
                     /locus_tag="ACHOQW_003614"
     CDS             complement(6081..9131)
                     /gene="fdnG"
                     /locus_tag="ACHOQW_003614"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8544..8546),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9574628.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQGNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9325..10158
                     /gene="fdhD"
                     /locus_tag="ACHOQW_003615"
     CDS             9325..10158
                     /gene="fdhD"
                     /locus_tag="ACHOQW_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP9574629.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2598 a         2444 c         2597 g         2596 t
ORIGIN      
        1 ggcatcattg tcagtatcat tcatttactg ctctggatgt tataaataat tatgtagcat
       61 gtttgctctt aaaccgtggg aaggaggctg taatggttgc caatattaat ctcattaaac
      121 aagaaagtta ttctgttgtt aatttagaaa aacaactaat gagtaatgaa tcaccgaagc
      181 aatcgctatt aaatgatttt tcacatcgcc ttaaaatgga aggtgaggaa caactgttcc
      241 attgtgttaa cgaactgaag tatggtttgt cgagtaataa tgaatatgac ctgtttaaaa
      301 atagagaaac ctcactgtgg aaacgcaaag taccagaaag cgcagcgctg gcaatgacgg
      361 gaacagtata taaattattt ggtagtcgct tactcaacgc ccgcaatcag ttagataaaa
      421 atctcctcga aatggctatg caaatggttt atggcaaaga tatggtgaca aagtccgggg
      481 atatattgat tgatatacaa ttaaataagg atggcagcct ccagagtaag cactacacat
      541 ttgatgtagg tgaagttata aacagttata atattgacct ctttgtgaat gcttcgagta
      601 ttaatcaaac ttacttacat gataaaaatc ctggtgatgt tattagcctg tatggcactt
      661 tagataacat tcctcttgta gttaaggaat gcacgggtaa aaatcatcaa attaacgtta
      721 aagccaaatt gccacaagaa cgaagtgaaa aagttgagga tatgtgtgaa agaatgagag
      781 gtggcatctc tatgtttaat cacaccacaa aaacagctgg taatatagag cataatttgc
      841 agctaagttt tctcgtggat tcacggccgt cgatcaccaa taaatactca gccaaagatg
      901 tagcgccaga catacttgta ttgcccgtca acgtatatca tgcggatttt aaaattaaaa
      961 tcaataatgc agtagagaag acttagtcaa ttgtgttatg tgctgtgcaa aaaaatctcc
     1021 atgcagcaag gaaggttctt agcgggataa taggtaatgt accaaaaaat aataaaccaa
     1081 aaacaatcat ttttgattta cgttttctta gtgcaattaa gcagaaagtt tttttgggca
     1141 gtgaacaacc gttgtttgta aagcataccg caatgatgag ggaggcatgt aaggaattac
     1201 cgcagctagt ggagtatgtt cctttagcct gtgaagcgca tagccatcgc agcgtcgccc
     1261 ttgccaaaac cgtggctgca cccatcatgg cgacaggtgt gggcgcggct ttaatgtatg
     1321 caacatcatc aggaccgtat tatgcgcaaa gtctttctgg ttcgccggat atcgagatga
     1381 taaaaaaacg catggctgaa ttgtttaacc agtcggatac ttctgtgcgc aacaaatatc
     1441 gcccggcttt tgataaatgg aataaactgg tagataaatt aaatcacgaa agaaatacag
     1501 ttccgtttgc gtgtttgacc accattatag cgacagcgat aaatgaaact cctgaggggg
     1561 atacgaatgt tgccgttgtg atggggtgta aatctgcgaa agatcgcacg atatccattg
     1621 tccttggcaa ctcgatgttg caaacactct ttgaaaaacg cctcgccgat gatggagaaa
     1681 tagaaaacct ttttgatcaa cagggatatt ttagttgcga ttcattaaca gccgaagaag
     1741 taatgatgtt aaaagacctt tttgatattc gagttcttca tctctctaac aaatttaatg
     1801 ttgggttgca aggtaatatc aacacggatg ttttacaaga ttcgtttttt aaaaatgtcg
     1861 atttcattca tgaatctaac tcgtataccg tagggatggg agtctgaaga gtaaaactca
     1921 ttaggattat cagaattcgg ccaatgcatt gcaaaaatga aatacaaaga gagatgtgtg
     1981 caggcgctta gccgcgaggg gcatggcgaa gccatacaga aagcaccggc attcagaact
     2041 cctgtgaaag cggcatagtg ccgctgctca ggagttttgt tatgacccgt atcgtgctga
     2101 acgttgccac cctatgatgc gattaaaaaa actcaccgtc gagccgatga ttgacgtcaa
     2161 tgctatcgga tgatgccggg ggtgttccgg aaggaattcc ttgtttcccg aactgttacc
     2221 aacaaaatta taacgatgag caagtaaaga ccggttggtg gcagtactga ccggtctgtg
     2281 atggaagatt aatttcacct taaatcctgg tgctgttagg atgctgacgt ttttcgggta
     2341 caccattcag attatgcaaa taaagattca atgaaaaaat aaatatccct gaatataata
     2401 tattcgtagc acccataact catcctgggc gataactaaa ccatttttga gtaacaaatg
     2461 aaacaactgc aacgctctta ttatttctgg aaaacgtctc gcaaaaacaa aacaacatgc
     2521 aaaaatccgc tttcgttaat taatttttcg gcgtaatttt cgcacataca ttaatgagta
     2581 atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta tcagaagagg
     2641 caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct gcaattttac
     2701 gcgaagcggt agagcaatat ctggaacgtc agcaattccc ggtgcgtaaa gcaaagggag
     2761 gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat aaggaatgac
     2821 aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat taatagaagg
     2881 ctgttttact tgaacttata ataactgcaa ctgttacatc atatctggaa aacgcctcgc
     2941 aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcgacaagg gtacttttct
     3001 ctccatagat atcaaatgat attggtcaaa tatttatgaa ctctctggca ggtattgata
     3061 tgggcagaat tatactcgat ttatcgaatg aggtgattaa gcaacttgat gatcttgagg
     3121 tgcagcgtaa tcttcctcgt acggagctat tacgggaagc ggtagatcaa tacttggtaa
     3181 atcaatcgca aacagtaaga accagtgctt ttggcatatg gcaagggtgt gaggaagatg
     3241 gtgtcgaata ccagcgtaag ctgcgcgagg aatggtaatg ggaaaaaatg atatcaatca
     3301 gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc gtgaacgcct tacccggcct
     3361 acaaagtcgt ataaattcaa tgtattgcag tttattggta ggcctaataa gcgcggcgca
     3421 tcaggcaatc ttacgcttat catcagtctc atacccataa ccttattgat gtgggggtgg
     3481 gtaaattgag gcaaaacagg cactggcggc atagcttgag gtggtaacgc ggaatacgag
     3541 agatggtgtc gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg
     3601 tcagagatta ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc
     3661 cttcttgctc cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa
     3721 ctttcggttc tttttcctga tagagaattt tcaggtaagt gccgcagtca tcgcagcttt
     3781 cggctttaat cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct
     3841 cgcagttgct gcattttacg cgcacaacgt gccattcagt ttcacacagg ttgcagtgca
     3901 ggtaacgtag cccctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac
     3961 aaaccgggca atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat
     4021 tggccatctg cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc
     4081 tgacggacga gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg
     4141 atgccttctc cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct
     4201 cagcaatcag cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat
     4261 ccagcggggg cttgccttgt gcgctggctt ctttaatgcg cgcagtcaga tccatctcca
     4321 gcggatggtc gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt
     4381 aatcacccag cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata
     4441 aattcttgag ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg
     4501 aacccagctc atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt
     4561 gcggacctca cggtaccagc gcgggtgatg tttcttcgcc caagcgctgg taacccatcc
     4621 ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat
     4681 aaccacaatt aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg
     4741 gattgagaaa gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag
     4801 aaccaggaaa ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt
     4861 gtcacctact tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc
     4921 ccgattgatt aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat
     4981 aaccacgccg acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat
     5041 caaccagttg aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca
     5101 gaaggcggtg atccagtggt tgatacgttc cggcgcggtg tagcgcacga tggtgtcacg
     5161 tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca
     5221 tggttcggac cgacacccac gtagtggaag atactggctg cgaaggtagc cgcaaagcca
     5281 acagctgcga gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg
     5341 ttctccggca agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacataacg
     5401 tgtgtaccac cgacgcctgc cggatcgtac aggcccgcat tgtcgtaacc gcgggttttc
     5461 agctccgcca cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc
     5521 gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg
     5581 cacagcgtac atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga
     5641 cagcccgcaa tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg
     5701 ttggcatact gaataattgc accttccgcc gggcacgctt tcagacagcc tggatcggaa
     5761 cagtgcatac agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc
     5821 gagaagcgca tcaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca
     5881 atgttattgc cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct
     5941 ttacagccga tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc
     6001 gcctgaggcg cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga
     6061 taagccatat gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc
     6121 ggcgtctgcg tgttcgcatc accgacgaat ggcgtcaacg tattggcaat aaagcctttt
     6181 ttcgcaacac cttcatagcc ccagtgaata ggaataccga tggtatcgat atctttgccg
     6241 ttcgctttca gcgtgcgaat acgtttggtc accaccgctt tggctttgat atagccacgg
     6301 ttggaggaga ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc
     6361 ccgatttcca caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag
     6421 tagtggaagt gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta
     6481 cccaatgctt cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga
     6541 tgcagcgggt tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct
     6601 tccgccatct tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc
     6661 ccgacgccgc tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta
     6721 gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg
     6781 gaggcgcggt tatacagaat gcggcggtta agcggccatg cccatgccca acccagcgtg
     6841 ttaccgaggc cagacggatc ggcgttatca cggcgtgcca tctggttgcc ttccggcgtc
     6901 cagctaccgg cgaaaatcca gcaaccacag gaagttgtac cgtcatcgcg cagttgggca
     6961 aacgagctaa gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata
     7021 tcggccagcg ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga
     7081 atggcgtagt tccaggtcat gttcagcacc tggtccgggt tcgcgccacc ctgttcggca
     7141 tacatcttgc gcaagcgcag gaagataccg gagaggatct cgccatcagt cagcgcaatc
     7201 cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa
     7261 ccgttctctt ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac
     7321 gagtcaactt cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc
     7381 gggtcgatgg tgacgaggaa cttcagtttc gacagacaac tgatcacttt gtttttgttc
     7441 gggaatgagg caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc
     7501 atcatctcga agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag
     7561 ccccagctat tttctgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag
     7621 aatttcgggt agttgcccca gtagtttccc tggccttcca gcagcggttt tggcgtgttg
     7681 gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc
     7741 aggctctgcg acagcagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg
     7801 gcgttaacgc cgccgccagc catccccatg ttaccgagca gcagctggat catcgccatc
     7861 gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag
     7921 gcagttttat cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct
     7981 tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc
     8041 agcaagttcc acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt
     8101 tcgtccagtt cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg
     8161 gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga tcaggctggc gttggtatag
     8221 gcttcagtgt attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac
     8281 aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt agtcagccac cgcagccgta
     8341 cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccat tgtgaatttt ggcttccatc
     8401 gcccagcgga acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg
     8461 ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca
     8521 agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc
     8581 agcgcgcggg agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac
     8641 agcatcccgg tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag
     8701 ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg
     8761 atttgctgcc atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg
     8821 gagtggatga aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg
     8881 tgatccggat cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg
     8941 aggctgtaca tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg
     9001 cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg ctacgctggg tgcaaaaccc
     9061 agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg
     9121 ctgacctgca tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc
     9181 tgcgtgtgca gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat
     9241 tggctcaatt tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt
     9301 gtgttcttat ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg
     9361 acaaatatca caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca
     9421 cggttagatg aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat
     9481 gtggtgatga tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc
     9541 gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat
     9601 ggtcttgaag tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc
     9661 cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc
     9721 ggaaaaccgg tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat
     9781 gcattacgtc atctcaacga ttttcagcca gtgggacaac tgaccggttg tactcacgcc
     9841 gccgcctgga tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat
     9901 gtggcgctgg acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt
     9961 gcggtactgg tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc
    10021 gtagagattt tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgc
    10081 tgtaatctga cactggtagg tttttgtaaa ccgggtcggg caacggttta tacccatccg
    10141 cagcgtttga gcaattaatt aaaataagtc ctgcgatata tattaaattg caggacttat
    10201 tcatttcgtg aattttatta ttttatttat aaaca
//
LOCUS       DAZKFZ010000166        10209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000166 DAZKFZ010000000
VERSION     DAZKFZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            243..4496
                     /gene="fdeC"
                     /locus_tag="ACHOQW_003616"
     CDS             243..4496
                     /gene="fdeC"
                     /locus_tag="ACHOQW_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP9574630.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            complement(4617..5474)
                     /locus_tag="ACHOQW_003617"
     CDS             complement(4617..5474)
                     /locus_tag="ACHOQW_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9574631.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5723..6592
                     /locus_tag="ACHOQW_003618"
     CDS             5723..6592
                     /locus_tag="ACHOQW_003618"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9574632.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6752..7345)
                     /gene="rclC"
                     /locus_tag="ACHOQW_003619"
     CDS             complement(6752..7345)
                     /gene="rclC"
                     /locus_tag="ACHOQW_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP9574633.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(7357..7593)
                     /gene="rclB"
                     /locus_tag="ACHOQW_003620"
     CDS             complement(7357..7593)
                     /gene="rclB"
                     /locus_tag="ACHOQW_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP9574634.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(7702..9027)
                     /gene="rclA"
                     /locus_tag="ACHOQW_003621"
     CDS             complement(7702..9027)
                     /gene="rclA"
                     /locus_tag="ACHOQW_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP9574635.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            9253..10107
                     /gene="rclR"
                     /locus_tag="ACHOQW_003622"
     CDS             9253..10107
                     /gene="rclR"
                     /locus_tag="ACHOQW_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP9574636.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         2760 a         2289 c         2530 g         2630 t
ORIGIN      
        1 atttaatgac ttgcacaaaa agctaaattt tcacccataa ataaaaatat aatcccgcgc
       61 ccaaccacct gatgagtggc tacaggcact ggatatatta ggtggcggtg tactttctta
      121 cataaaggta tttccttttc tgcggaaaag gaaaccggga aatccccggt ttttctgaca
      181 agcagacgcc attatttgtg tctgcctatg ttcgttaatt cgttcatcag gaaattatct
      241 caatgtcacg ttataaaaca ggtcataaac aaccacgatt tcgttattca gttctggccc
      301 gctgcgtggc gtgggcaaat atctctgttc aggttctttt tccactcgct gtcaccttta
      361 ccccagtaat ggcggcacgt gcgcagcatg cggttcagcc acggttgagc atgggaaata
      421 ctacggtaac tgctgataat aacgtggaga aaaatgtcgc gtcgtttgcc gcaaatgccg
      481 ggacattttt aagcagtcag ccagatagcg atgcgacacg taattttatt accggaatgg
      541 ccaccgctaa agctaaccag gaaatacagg agtggctcgg gaaatatggt acagcgcgcg
      601 tcaaactgaa tgtcgataaa gatttctcgc tgaaggattc ttcgctggaa atgctttatc
      661 cgatttatga tacgccgaca aatatgttgt tcactcaggg ggcaatacat cgtacagacg
      721 atcgtactca gtcaaatatt ggttttggct ggcgtcattt ttcaggaaat gactggatgg
      781 cgggggtgaa cacctttatc gaccatgatt tatcccgtag tcatacccgc attggtgttg
      841 gtgcggaata ctggcgcgat tatctgaaac tgagcgccaa tggttatatt cgggcttctg
      901 gctggaaaaa atcgccggat attgaggatt atcaggaacg cccggcgaat ggttgggata
      961 tccgcgcaga gggctattta cctgcctggc cgcagcttgg cgcaagcctg atgtatgaac
     1021 agtattatgg cgatgaagtc gggctgtttg gtaaagataa gcgccagaaa gacccgcatg
     1081 ctatttctgc cgaggtgacc tatacgccag tgcctcttct gacactgagc gccgggcata
     1141 agcagggcaa gagcggtgag aatgacactc gctttggcct ggaagttaac taccgaattg
     1201 gcgaaccttt ggcgaaacaa ctcgatacgg atagcattcg cgagcgtcgg gtactggcag
     1261 gcagccgcta tgacctggtt gagcgtaata acaacatcgt tcttgagtac cgcaaatctg
     1321 aagtgatccg tattgctctg cctgagcgta ttgaaggtaa gggcggtcag acactttccc
     1381 tggggcttgt ggtcagcaaa gcaactcacg gactgaaaaa tgtgcagtgg gaagcgccgt
     1441 cattactggc tgaaggtggc aaaattaccg gtcagggtag tcagtggcaa gtaacgctcc
     1501 cggcttatcg tccaggcaaa gacaattatt atgcgatttc agcagttgcc tacgataaca
     1561 aaggcaatac ctcaaaacgc gtgcagacag aggtggtcat taccggagct ggtatgagcg
     1621 ccgatcgcac ggcgttaacg cttgacggtc agagccgtat tcaaatgctt gctaacggta
     1681 atgagcaaaa accgctggtg ctgtctctgc gcgacgccga gggccagcca gtcacgggca
     1741 tgaaagatca gatcaagact gaactaactt tcaaaccggc tggaaatatt gtgactcgtt
     1801 ccctgaaggc cactaaatca caggcaaagc caacactggg tgagttcacc gaaactgaag
     1861 caggggtgta tcagtctgtc tttactaccg gaacgcagtc aggtgaggca acgattactg
     1921 ttagcgttga tggcatgagc aaaaccgtca ctgcagaact gcgggccacg atgatggatg
     1981 tggcaaactc caccctgagc gctaacgagc cgtcaggtga cgtggttgct gatggtcagc
     2041 aagcctatac gttgacgttg actgcggtgg actccgaggg taatccggtg acgggagaag
     2101 ccagccgctt gcgatttgtt ccgcaagaca ctaatggtgt aaccgttggt gccatttcgg
     2161 aaataaaacc aggcgtttac agcgccgcgg tttcttcgac ccgtgccgga aacgttgttg
     2221 tgcgtgcttt cagcgagcag tatcagctgg gcacattaca acaaacgctg aagtttgttg
     2281 ccgggccgct tgatgcagca cattcgtcca tcaccctgaa tcctgataaa ccggtggttg
     2341 gggggacagt tacggcaatc tggacggtaa aagatgccta tgacaaccct gtgaccagcc
     2401 tcacgccgga agcgccgtca ttagcgggtg ccgctgctga aggttctacg gcatcgggct
     2461 ggacaaataa tggtgatggg acgtggactg cgcagattac tctcggctct acggcgggtg
     2521 aattagaagt tatgccgaag ctaaatggac agaatgcggc agcaaatgcg gcaaaagtaa
     2581 ccgtggtggc tgatgcgtta tcttcaaacc agtcgaaagt ctctgtcgca gaagatcacg
     2641 taaaagccgg cgaaagcaca accgtgacgc tggtggcgaa agatgcgcat ggcaacgcta
     2701 tcagtggtct tgcgttgtcg gcaagtttga cggggaccgc ctctgaaggg gcgaccgttt
     2761 ccagttggac cgaaaaaggt aacggttcct atgttgctac gttgactaca ggtggaaaga
     2821 cgggcgagct tcgcgtcatg cctctcttca acggccagcc agcagccacc gaagccgcgc
     2881 agttgacggt catcgccgga gagatgtcat cagcgaactc tacgcttgtt gcggacaata
     2941 aggctccgac cgtcaaaacg acgacggaac tcaccttcac cgtgaaggat gcgtacggga
     3001 acccggtcac cgggctgaag ccagatgcac cagtgtttag cggtgccgcc agcacgggga
     3061 gtgagcgtcc ttcagcagga aactggacag agaaaggtaa tggggtctac gtgtcgacct
     3121 taacgctggg atctgccgcg ggtcagttgt ctgtgatgcc gcgagtgaac ggccaaaatg
     3181 ccgttgctca gccactggtg ctgaacgttg caggtgacgc atctaaggct gagattcgtg
     3241 atatgacagt gaaggttaat aaccaactgg ctaatggaca gtctgctaac cagataaccc
     3301 tgaccgttgt ggacacctat ggtaacccgt tgcaggggca ggaagttacg ctgactttac
     3361 cgcagggtgt gaccagcaag acggggaata cagtaacaac taatgcggca ggtaaagcgg
     3421 acattgagct tatgtcaacg gttgcgggag aacacaatat ttccgcttcg gtgaatggtg
     3481 ctcagaagac ggtcacggtg aaattcaacg cggatgccag caccggtcag gcaaacctgc
     3541 aggtagacgc cgctgctcaa aaagtggcaa acggcaaaga tgcctttacg ctgacggcga
     3601 acgttgagga taaaaatggt aaccctgttc cagggagcct ggtgaccttt aatctgcccc
     3661 ggggtgtcaa gccgcttaca ggcgataatg tctgggtgaa agccaacgat gaggggaaag
     3721 cagagttgca ggtggtttca gtgactgccg gaacgtatga gatcacggca tcggcaggga
     3781 atagccagcc ttcgaatacg cagactataa cgtttgtagc cgataaggct accgcaaccg
     3841 tctccggtat tgaggtgatt ggcaactatg cactggcgga cggcaatgcc aaacagacgt
     3901 ataaagttac ggtgactgat gccaataaca acctgttgaa agatagcgaa gtgacgctga
     3961 ctgccagccc ggcaaattta gttctgactc ccaatgggac ggcgaaaact aatgagcaag
     4021 gacaggctat tttcaccgcc acgaccactg tcgcagcgaa atatacactc acggcgaaag
     4081 tgagtcaggc cgacggtcag gaatcgacga aaactgccga atctaaattc gtcgcggatg
     4141 ataaaaatgc agtactcacc gcatcatctg atgtgacttc tctggtggcg gatgggatat
     4201 cgactgcgaa gctggaggtg acactgatgt cggcaaataa ccccgttggg gggaatatgt
     4261 gggtcgacat taagacgcca gaaggggtga cggagaagga ttatcagttc ctgccgtcga
     4321 aaaatgacca tttcgtgagc ggaaaaatca cgcgtacatt tagtaccagc aagcctggtg
     4381 tctatacgtt cacatttaac gccctgacgt atggcgggta cgaaatgaag ccagtgacgg
     4441 tgaccattac cgcggtggat gccgatacgg caaagggcga ggaggcgatg aactaatatt
     4501 aagaatatgg aagtaacatt ctaataaaca ataatggcgt tgacatcttc aacgccatta
     4561 tttattgaat tgcctgacag tagtctcgtt ataagttcat ttagatatag cgacagctaa
     4621 accggacgaa cagggataaa tatttcgcaa acaacatcat tgctatcctt ttctacgaaa
     4681 tcttcattgt aataaaaccg ctcaatatca ctgccgttca ctctggctaa ccccctggcg
     4741 ggtaattcca tcagatagag gtttcgcgcc cattcagggt agtgctccca ctcaccttca
     4801 tagcggaaag aggcatacag tcctcggtta attgtccact ctttcggcat ctctgaatcg
     4861 gtttgtttgc ataccgtacc ggcaaaagtt tcaactgtga gtaaatcgac cgattttgtt
     4921 gacggttcaa acgtggagac gaaatagata tccgtcttaa aattcttcat acatctggct
     4981 aaatgatgac gtcgcagttt gacttcttca tccggtgatt tattcgacac ttcaatatca
     5041 tatttaaatg aattgccaaa aataggtgtc tcaggaaagt tgactaatct acactccgtc
     5101 ttttgctgtt gacgtattaa aaaagaaggg aagatatttg cgagatccca ataatcacgg
     5161 tggcggtata cacggggaga gcagccgaat aactttttaa attcgcggct gaatgactgc
     5221 tgtgaatcaa aatgcaaaga gattgcaata tcaagcatag atttcgcggt gagccggaca
     5281 agaatggcgg cacgacaaag ccttcgtttg cgaatgtatt ctcccaaagg cacatgcatg
     5341 aaatttcgga acagaagctg gatgttgcgt ctgctgtagc cagatttctg tgcgatatct
     5401 tcgattgaaa tagggtgctc aagattgcac tcaatccact ccaggagctg ctgtagaatc
     5461 ttctgcctga tcatcacatt ctccgttaat tctttgtcat atcgcacatt ttcgttatgc
     5521 cctgggccct aagtgtatca aaaacagcaa gggcgaaggc aaaagcactg gtagcgtctg
     5581 cttcgttgtc cccgaaaata gagaggtttc agtcctacat tattaatgaa ttttttgcat
     5641 aagtgatatc aaaatccaca tactaatttg aggttacgtt ttaacgtaga ctcattgttc
     5701 atgcctaatg gagggactga cagtggaatt ttccgtatta agtaacaatc tgaaaatgcc
     5761 gatgatggga tttggtgttt ttcaggttac cgataaaaac gtgtgcaaac agtcagtgtt
     5821 gaacgctatc cgcacggggt atcgactcat cgacactgca gcggtatacg gtaatgaaga
     5881 tgccgtcggt gaagccgttc gtgaagctat atccgaaggt ttatgtaccc gagaagagtt
     5941 atttataacg tctaaattgt gggtgcagga tatgttgaat caggatacgg cagcagcagg
     6001 tattgaagca tcattaaaaa aatcgggact agaatacttc gacctctatt tattgcatca
     6061 ggctatgcgc gattatttca gtgcgtggcg tgcactcgaa gatgcctatg aagagggcaa
     6121 attaaaagcg attggggttt ccaatttcta tcctcatgtt ctggcgaact tttgtgaaac
     6181 ggtaagagtt aaaccgatgg tcaaccaggt cgagttgcat ccctattttg cccaaccaga
     6241 ggcgctggca accatgaagt attataacgt gcagcctgaa gcatgggctc cgttaggtgg
     6301 tggacgacat aaaccctttg aaaataatct gcttcagagt attgcagatg cccatcaaaa
     6361 atcgatttct caagtcattc tgcgttggaa tattcaacgg ggagtggtcg ttattccgaa
     6421 atcgacacat caacagcgta tcgaagaaaa ttttgctatc tgggatttct cactgacaga
     6481 gaaagaaatg gcacaaatta gttcgcttga tttgggttat gttggggagt cggtaaaaca
     6541 ttttaatcct gaatttgttc gtggttgtct tgctgtaaaa atacatgatt gatattaatc
     6601 atgtatttta cctgagacga caagaatctt ttaacagggg agtgatattg atcttcactc
     6661 tgtcatatct ccggtaatat ggcgtcaggc ttcatcatca gaaagggggc ctgacgcctg
     6721 aaaaagtgaa caacagacag tgttcggatt atcaatattc agtttttaac gttgaactgg
     6781 attcattact gccttgttta agaatttccc gcgccgaatc tgccattatc atgacagcac
     6841 ctgccagcat cagagtatct ttcaatacca gacgaccagc accggataaa taagggaaac
     6901 catgatgagc gtcacccaat gcgggtaccc atgcctccgg ggtggtgatt aaaaatgaga
     6961 gtgttaccag cggtgtggta aatgccatca gccctcccaa taaacctaac cagcgattga
     7021 caggattagc caaaaccagc agagcaataa tcacctccac gacgccaaga ccgttggaaa
     7081 aaccataggt attattggcc gattgccatg cccttgcttc tggtttgtat tcgccttcgt
     7141 gagtcagata ctgtttatag tcttccgggt gttcataaaa gaacgacatt agtggactgt
     7201 ttgcgacgaa tggtgtaatg ctgtctgcct cgtaagggac aaactttaat aacccaatcc
     7261 acataaaaac aatcgcaata ctcagacgaa tcaatgccag gccaatttta tctccccgac
     7321 ttaacaggtg caggtatttt tccattgtga atatccttat ttatacaata ctgctgttgc
     7381 ggttgaatta ttatcaattc gcatcgatgt gactttccag gatgaagcat tatattttct
     7441 ggctatatca gcaatggcat cctccagaac tgtcggtgat gctgatactg acgagacgct
     7501 gatatgtttc agaattattt tatcatctgc attggtggaa aatgccataa cgccagataa
     7561 tagtgttgca aataaaacgg attttttaaa catataaaat accatcaatg aagtgattaa
     7621 gaagttatag agtaacaggg aattaataag attcttttct ctgagacgcc agaatatttg
     7681 ttctggcgtc tgattttgag tttatttgac taatgaaaat agatcattga gtgattcgct
     7741 catcgacgga tgagtaaata tctgatcgcg taatatgcta taaggcagcc cggcatccat
     7801 caccattttc actatattaa tcatctcgtg ggagtcaaca cacagcagtg atgcccctaa
     7861 tatacgttgg gttttattat caacaatcgc ttttaatacc ccacgggtat cgttcatcac
     7921 tctggcgcgc ggaattgcag ctacaggcaa tgtcaccacc tgaatatcag caccactctc
     7981 tctggcttgt tcttctgtca taccaaccct ggacaggggc ggtgtcataa atacggaata
     8041 aggcacattt ttccgatcat cagtactacg tttgccttca cccagtaact catcacgtac
     8101 aatgcggtaa tcatccagtg atatgtaagt aaattgcagc ccgccggtaa catctcccat
     8161 cgcccaaata ttgtctgcgg tggtatgtaa tcgcttgtca acgacaattg ccccgcgctc
     8221 gtttactgcg ataccggcat tttctggatg taacgaagcg gtagccggtt gacgaccgga
     8281 agctattaac agtgcatcca ccgccagttg ggcgtgctcg ctatgcactt gcacttgatt
     8341 ttcatggtga ctgattcgct ccacatgggc attgaggata atatcgacgc cctgatcgcg
     8401 taaaatcgtc gcgatattat cagcaatatc ccgttcttcc cgaggcaaaa acagcgaagc
     8461 tgcttctaaa atggttacct tgctgccaaa attagcgaac atagaggcga actcaacgcc
     8521 aatatatccg ccgcccaaaa tacctaaatg cccaggcaat tcttttagat taagtaatcc
     8581 ggtgctgtca tatactcctg gcgtggtggt aattccagga attggcggaa ccacggtttg
     8641 tgcaccggta ttaataaaaa ttttctcgcc atgaatttcc agatttccct caggccgatg
     8701 aacacgcagg ctatgattat tgataaactc cgcctggccg ttgatcacgt cgatattggg
     8761 catatctgca agattatgaa aattcttatt acgtaaaaaa ttaaccactt catttttacg
     8821 ctgtatggct cggacaaaat ctgtgtgctg ctgtgcgtca tgaaccaatg ttttggttgg
     8881 gatgcagccg atattaatac aggtcccgcc atacattgca tttgattgtt cgatgagagc
     8941 cactcgccaa cctgcttttg ccagcgtgac ggctaatgtt tttccagcct tgccaaaacc
     9001 aataatcact gcctgatatt tattcatgat gatttcctgt cgaaacgcgt tcatttgaca
     9061 tcatgactat agacgcaaaa gagcaaataa catttcttca caacgcttga tattttgtct
     9121 tatcgtctgg tattttcttt ttcttaatca gaatcacaaa acaaaaatca tgattaacta
     9181 tttgatggta ttagattagt tgtttactaa gattgttggt gtttgtaatc aaaaaccact
     9241 caggagtctg atatggatgc ccttagccgt ttgttgatgc ttaacgctcc acaaggaacg
     9301 atcgataaga attgcgtgtt aggaagtgac tggcagcttc cacatggtgc cggggaatta
     9361 tcagttattc gttggcatgc gttaacgcaa ggagcggcga agctggaaat gccgacggga
     9421 gagattttta cattacgccc gggaaatgtg gtcctgctac cacaaaattc agctcatcgc
     9481 ctgagtcatg tcgataatga atcgacctgt attgtctgtg gtactcttcg gctgcaacat
     9541 tcggcacgtt attttttaac gtctttgccg gaaacgctgt ttttagcacc ggttaaccac
     9601 agcgtggaat ataactggct gagggaggcg atcccgtttt tacaacagga atccagatcg
     9661 gcaatgccgg gagtggatgc actgtgtagc cagatctgcg ctacattctt taccctcgcg
     9721 gtgcgtgagt ggattgcaca ggttaatact gagaaaaaca ttctcagttt gcttctgcat
     9781 ccacgtcttg gtgcggtaat acagcaaatg ctggaaatgc caggacacgc ctggagcgtc
     9841 gaatcgctgg ccagcatcgc ccacatgtcc cgggcaagtt ttgcccagct tttccgtgat
     9901 gtttccggaa ccacgccgct ggctgtatta acaaagttgc gtctacaaat agcggcccag
     9961 atgttttccc gggaaatgct ccctgttgtg gtgatcgctg agtcagtagg ctatgccagt
    10021 gaatcatctt ttcacaaggc gtttgtccgc gagtttggtt gtactccggg agaatatcgg
    10081 gaaagggtca gacagcttgc accctgaata aaaccgccag aaatcagggc aaagataatc
    10141 cgcattccgg gagttgtgag cctttcaact atttctattt ccaacggtgg tttgggcttt
    10201 atatatttt
//
LOCUS       DAZKFZ010000167        10206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000167 DAZKFZ010000000
VERSION     DAZKFZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..686
                     /locus_tag="ACHOQW_003623"
     CDS             3..686
                     /locus_tag="ACHOQW_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574637.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            702..1112
                     /locus_tag="ACHOQW_003624"
     CDS             702..1112
                     /locus_tag="ACHOQW_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574638.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1333..2154
                     /locus_tag="ACHOQW_003625"
     CDS             1333..2154
                     /locus_tag="ACHOQW_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9574639.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2236..2715
                     /locus_tag="ACHOQW_003626"
     CDS             2236..2715
                     /locus_tag="ACHOQW_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9574640.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            2730..3206
                     /gene="yeeS"
                     /locus_tag="ACHOQW_003627"
     CDS             2730..3206
                     /gene="yeeS"
                     /locus_tag="ACHOQW_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9574641.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3269..3490
                     /gene="yeeT"
                     /locus_tag="ACHOQW_003628"
     CDS             3269..3490
                     /gene="yeeT"
                     /locus_tag="ACHOQW_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9574642.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3564..3932
                     /gene="cbeA"
                     /locus_tag="ACHOQW_003629"
     CDS             3564..3932
                     /gene="cbeA"
                     /locus_tag="ACHOQW_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFP9574643.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4021..4394
                     /gene="cbtA"
                     /locus_tag="ACHOQW_003630"
                     /pseudo
     CDS             4021..4394
                     /gene="cbtA"
                     /locus_tag="ACHOQW_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4391..4585
                     /gene="yeeW"
                     /locus_tag="ACHOQW_003631"
     CDS             4391..4585
                     /gene="yeeW"
                     /locus_tag="ACHOQW_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9574644.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4631..4711
                     /locus_tag="ACHOQW_003632"
                     /pseudo
     CDS             4631..4711
                     /locus_tag="ACHOQW_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5000..5144)
                     /locus_tag="ACHOQW_003633"
                     /pseudo
     CDS             complement(5000..5144)
                     /locus_tag="ACHOQW_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5198..5380
                     /locus_tag="ACHOQW_003634"
     CDS             5198..5380
                     /locus_tag="ACHOQW_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP9574645.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5481..5810)
                     /gene="tmaR"
                     /locus_tag="ACHOQW_003635"
     CDS             complement(5481..5810)
                     /gene="tmaR"
                     /locus_tag="ACHOQW_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP9574646.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5982..7040)
                     /gene="yeeA"
                     /locus_tag="ACHOQW_003636"
     CDS             complement(5982..7040)
                     /gene="yeeA"
                     /locus_tag="ACHOQW_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9574647.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7239..7712)
                     /gene="sbmC"
                     /locus_tag="ACHOQW_003637"
     CDS             complement(7239..7712)
                     /gene="sbmC"
                     /locus_tag="ACHOQW_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP9574648.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7831..8997)
                     /gene="dacD"
                     /locus_tag="ACHOQW_003638"
     CDS             complement(7831..8997)
                     /gene="dacD"
                     /locus_tag="ACHOQW_003638"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP9574649.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            complement(9837..9994)
                     /locus_tag="ACHOQW_003639"
                     /pseudo
     CDS             complement(9837..9994)
                     /locus_tag="ACHOQW_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
BASE COUNT         2682 a         2591 c         2381 g         2552 t
ORIGIN      
        1 ttatgaaatt acctgtaaag ttattaatga gccttatatc tctggtccgc gttattgcac
       61 gtgccgggga atataaaaat tacagccggg atgaaataaa atactggcga tatacatcat
      121 acaagggggg gaaacttccg gaaggtttca ctgatgagaa attttccagc gccatttaca
      181 acggaagaat atttacaatg aaacgtttac atacccttat gttatttctg gcggttctgt
      241 tcacgggctt taactttaac gcagaagcgg ctaccgtaaa acaagctctt agttgtaacc
      301 cggaagcctg ggctgaacaa cctggagcgt gtccatcaac gtacgagttg tacgaaggtg
      361 acgctaccta caaagccgct atcgacaaag cattaaaacc ggtcggactg agcggcatgt
      421 tcggtaaagg cggctatatg gatggccctg gtggaggtat cacgccagta aacattaacg
      481 gtacagtctg gttccagggc gacggttgca aagccaatac ctgcggctgg gactttatcg
      541 taacactcta taacccaaaa acccatgaag ttgttggcta ccgttacttt ggtttagatg
      601 acccggccta cctggtttgg ttcggtgaaa ttggcgtgca tgaattcgcg tatctggtga
      661 aaaactacgt agctgcggtt aactaattta aggagtctat catgaaaact tttatcaaaa
      721 ctttactcgt tgctgtaact attctgttct ctgtcttcgc tactgcgaaa caagtaaaac
      781 tgccaaataa catcaaatac gttaatacta cagaagcgtt ttcctgtacc gaaattgacg
      841 gtatgaattg ccagacaaag aatcagttca actataaaga taacagctat gttttcgtgc
      901 ttgaacgtgg tggtgcctgg tgctacgact acactgtctc ggtagttaac ctgaaaaccg
      961 ggaaagcaca aatgattgaa tacggggaca atcaactgtg ctcaggtagc aacaaaccgt
     1021 tcttcgaaat caaaaatggc gtaccgacgg taggagtcat cgacacatcc ggaaaacctg
     1081 tcggtgtagc ccaggacaaa cttaaaatct aaggagtaaa ccatgcgttt ctctgcacat
     1141 tattaatatc aaaggtaaat acagccccag ccattgttac cggaaagccg cttaatgcgt
     1201 ggctcgccag tgaaaggcct ctcagacaat aaaccttatt catttcaccc gtcaggccgc
     1261 gtcttctccg ggagacgcgg cttttttcat ttattgcacc agtaaatctt aaccaccgat
     1321 aaggagcaaa gtatgcgatt agccagtcgt tttggttatg ctgcaaacca gatacgccgt
     1381 gaccgtccgc tgacacatga agaactgata cgccatgtac ccagtatttt tggggaagac
     1441 cggcacacct cccgcagtga acggtatgcg tacattccca ccattaccgt cctggaaaat
     1501 ctgcagcggg aaggctttca gccgttcttc gcctgccaga cccgtgtgcg cgacccgggc
     1561 cgccggggat acacaaaaca catgctgcgt ctgcggcggg ccggagagat aaacggagaa
     1621 catgtccctg aaattattct gctcaactct catgacggta cctccagcta ccagatgctg
     1681 ccgggttact tcaggttcgt ctgccagaac gggtgcgtct gtggccagtc tctgggggaa
     1741 gtgcgtgttc cacaccgggg aaatgtagtg gagaaagtta tcgaaggggc ttacgaggtg
     1801 gtgggcgtgt ttgaccggat agaggagaag cgtgatgcca tgcagtcgct ggtcctgccg
     1861 caaccggcac gccaggcgct ggcacaggcg gcactgactt accgttatgg tgacgaacat
     1921 cagcccgtca ccaccgccga cattctgacg ccgcgacgcc gggaggatta cggtaaggac
     1981 ctgtggagcg catatcagac catccaggag aatatgctga aaggcgggat ttccggtcgc
     2041 agtgcaaaag gaaaacgtat ccatacccgg gccattcaca gcattgatac cgacattaag
     2101 ctcaatcgcg cattgtgggt gatggcagaa acgatgctgg agagcctgcg ctgatgccgt
     2161 ttccctgagt gagggaatca ttcagctttc gttcccgaaa aacacacatt tatttgttat
     2221 ttaaggagtt atctcatgaa aaccgtttct cagaataccc ccacaattta ttcagctaca
     2281 acaccagaga ataatccgcc tcagttggtt gccagcctcg tccctgatga acagcgcatc
     2341 agcttctggc cgcagcattt tggcctcatt ccacagtggg tcaccctgga gccccgtgtc
     2401 ttcggctgga tggaccgtct gtgcgaagac tactgcggtg gtatctggaa tctgtacacc
     2461 ctgaacaacg gcggggcatt tatggccccc gaaccggatg atgatgatga cgaaacatgg
     2521 gtactgttca atgccatgaa cggtaaccgc gctgaaatga gcccggaagc cgccggtatt
     2581 gccgcctgtc tgatgacgta cagccatcat gcctgtcgta cggagaatta tgccatgacg
     2641 gtccattatt accggttgcg ggattacgcc ctgcagcatc cggaaggcag cgccattatg
     2701 cgcatcattg actgaaaggg gccggaataa tgcaacagat ttcctttctg cccggagaaa
     2761 tgacgcccgg cgagcgcagc ctcattcaac gggccctgaa aaccctggac cgccatcttc
     2821 atgaacccgg tgtggccttc acctccaccc gtgcagcacg ggaatggctg attctgaaca
     2881 tggcgggact ggagcgtgaa gagttccggg tgctgtatct gaacaaccag aatcagctga
     2941 ttgccggtga aaccctcttc accggcacca tcaaccgcac ggaagtccat ccccgggaag
     3001 tgattaaacg cgccctgtac cacaatgccg ctgccgtggt actggcacac aatcacccgt
     3061 ccggtgaagt cacacccagt aaggcagacc ggcttatcac cgaacgtctg gtacaggcac
     3121 tgggcctggt ggatatccgg gtgccggacc atctgatagt cggtggcaac caggttttct
     3181 cctttgccga acatggtctg ctttaacccg tcacaaccac atcacacctg ttttcacttt
     3241 tatcttctgt cttcagaggt atcccattat gaaaattatc acccgtggtg aagccatgcg
     3301 tattcaccaa caacatccga catcccgtct ttttccgttc tgtaccggta aataccgctg
     3361 gcacggcagt gctgaagcgt ataccggtcg tgaagtgcag gatattcccg gtgtgctggc
     3421 cgtgtttgct gaacgccgta aggacagttt tggtccgtat gtccggctga tgagcgtcac
     3481 cctgaactga gtgggaattc tgatgagcag aattatcgcc actaccggac cattcttagc
     3541 cgattttctg taaggatttt atcgtgtcag acacactccc cgggacaaca cttcccgacg
     3601 acaatcacga ccgcccctgg tgggggctgc cctgcaccgt gacgccctgt ttcggggcac
     3661 gtctggtgca ggagggtaac cggttgcatt accttgccga ccgcgccggt atcagaggcc
     3721 tgttcagcga tgcagatgcg taccacccgg accaggcctt tccgctgctg atgaaacaac
     3781 tggaactcat gctcaccagc ggtgaactga atccccgcca tcagcatacc gtcacgctgt
     3841 atgcaaaagg gctgacctgc aaagccgata ccctcagcag ttgtggttac gtttatctgg
     3901 ctgtttatcc gacgcccgaa atgaaaaatt aactctccag aatagccttc tgctacggcc
     3961 tggtgttttc accacgccac ttttccattt ttatatctgc atatcaggaa aatcttcagt
     4021 atgaaaacat tacctgtatt acccgggcag gcggccagtt ctcgcccgtc tcctgttgaa
     4081 atctggcaga tactgctgtc ccgactgctg gaccagcact atggcctcac actgaatgac
     4141 acaccttttg ccgatgaacg tgtgattgag cagcatattg aggcaggcat ttcactgtgt
     4201 gatgcggtga actttctcgt ggaaaaatac gcgctggtgc taccgaccag ccgggattca
     4261 gcgcctgtac ccgctctcag ttaataaaca gcatcgatat cctccgggct cgcagggcga
     4321 ccggcctgat gacccgcgac aattacagaa cggtaaataa cattaccctg ggtaagtatc
     4381 cggaggcgaa atgatgacac tggaagccga cagcgttaac gtacaggcgc tggatatggg
     4441 gcacattgtc gttgacattg atggtgttaa tatcactgaa ctgattaata aggccgctga
     4501 aaacggttat tcactccgcg tggtggatga ccgtgactct accgaaacac cggcaactta
     4561 tgccagccct caccagttgc tgtaagacaa tgcagtgatg cagggtatag tgccattaac
     4621 cacagaaaca gcactgttgt cctgtattaa gcaggcaaga gaagatgccc ttaccctgcg
     4681 ccatctgcag cttctgcacc agaaccggtg aatctcactc aacaggcttc atttcccctg
     4741 acgccagcct gaatacagct ggcgttttca tttataaaca gaaaggaaaa ccgataataa
     4801 tggaatggac gccccgacca tgaagcaacg gtaggatatt actgataaac cttccagtgg
     4861 aggtgcgtta tggccgtttt aacgattagc attgacctga caaaaaatga attccaaatc
     4921 cacggtctgg gtaggaacag aaaaatctaa gctcagaaaa cggattaaag taagcgtcaa
     4981 cggagcaccg tattgacgct tatttattgg tgagaactac gttccatggc aggagttcgt
     5041 caacacggtt ggagggccat tccggcagta tgctcagaat atggcgcaga tacgcttccg
     5101 gttcaaccgt tcagacggca ggtgcctgct tttccggcaa caagcaggaa tctcagcgct
     5161 gaagcaggaa ttagaggtaa aaacgcccta ccgggccatg aatcacccgg tcattggagt
     5221 agtaaccaaa gcagatttag ccagcatgga acaaatatct ttggtgaaat gttggttacg
     5281 ggaagctgga gcgcacaacg tgttagtaac cagtgcggtt aataacaatg gagttacaga
     5341 actttttgcc ttgctgcata cagaagaagg ctgccgctaa tttcgatagg ttctcagatg
     5401 agaatgatga cggtgataca cagataagaa tcaatgagca atgagggttg ccgggcaacc
     5461 ctcattgaat aaaacaggaa ttacttcgct tcgccgtttt tcatttcgcc catagctttc
     5521 agctttttgg agatatcgcg gcgttctttg gagagctcgg catttttgat gatgtaatcg
     5581 tcaacgcgat cttcatagtc acctttcatg ctggcgatga tgccctggat tgcttcaacg
     5641 ctcatccctg gcttgatgta atcgctcagg ttgtccagca acaggacgcg cttctggttg
     5701 tcacggatct ttttctcaac gtcctgaatt tcacgttgca gtttgttctt acgacggaac
     5761 agacgaacaa attccagtac gtcctggaat gaaggcttgg tagtttccat ttttataccc
     5821 ctgataatgt gagagtcgga ttcgtttaag caaccgctat tcgttagggc caacattact
     5881 catagctgcc gcagatgaca atgcttttat cccttatcac tatcataccc tttatccttg
     5941 ctgaatcgaa gcagcagcaa gatgattctg aagttcagga attatttgcg caaggcccgg
     6001 caaatcagac tcgatagcaa ctcaagctga tgtgccgttt ccatgttcag ccacacataa
     6061 ccatagattg gtgtttccac aaccttcagg tcatggtggt tattcagcaa ctgacgcagc
     6121 tcttccacag catcgttcaa tttttccgta ttggcaaaaa ccggctgcgg attaccttcg
     6181 tacagcgcat gaacaaggct cagcagtatt tgctgcatca tgtgctgggt atcacgaagt
     6241 ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg cccagtatgc attgatttgc
     6301 aactccagca tacaaaccag attgcggtta atggtctgga taccttcata tatcgatttt
     6361 ggaatacgag tttctttgct ggcgggcgca atcagcccac gcattttcac ggcatcggtc
     6421 aggagttttt gtagatggct ttccagacgt gggcgttcga gtaagttcgg tgagaatgca
     6481 gattgataga cccgattata ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg
     6541 aacgcccgtt gtggccagat accggtaaac aacattgcca gtaaagagcc gaggatcaca
     6601 tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg gggaacccac aacaattgcc
     6661 agcgtcaccc caatcaataa tccttgatac ggtttcttgc ccagcgccag ccaaccgcaa
     6721 agaaacatcg ccgccgcgca ccagactaac atcagcggta acgaaattaa ctccagttgc
     6781 agagcgataa gacctaaaat cgaccccaac accgtaccgc caatacgttc gaaggcacga
     6841 gggacaacgt tcccccagaa cgagattggc cccataatca ccaccatggt gaccagcggc
     6901 caggtgcctt ccgggatagt aaacaggcgg ataatgagaa aagtgagcag gaatgccagc
     6961 gcgacccgag taccatgcac aatgcggtaa tggcggtata cccggatttc aaacgggctt
     7021 aatgacttat tggcacgcac gcagaaactc cgttaatgaa gattaaaagc gctcataaca
     7081 catctgtcgg tcagaaggcg cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta
     7141 atttcccggc aggagcgaaa aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa
     7201 agagtggtct tcgcgtcaac acaccgccct gagatgaatt agtgatgttt tggctgcacc
     7261 gcaacataca tttcgatatc ccagtaccca tcttccgcgc cattgttcaa gtaaacctcg
     7321 aagcagggct ttggtaacat ttcataagca ctgtcctgca agaggctatt aaagaactga
     7381 taccagggtt tagcaaaatc atcaccgact acacgagcca ccgccaccgc atattgacca
     7441 cctgaaattt ctgtcagaat gacgccctca ctgttttcgg ggagcgtaaa gttattcggt
     7501 accgtcacga cggtgtcgca gcgtaatttt tcggcgggtg tttcatccgg attgtcgtaa
     7561 tagacagcaa cccactcctt cggcacaata tttttgctat ctacccacat catcaactgc
     7621 tcaaaacctt tctttaccgt ctgttcccac gggccaacga gatggaaacc tgcaacggta
     7681 cgtttatctt cctgcttaat ctcgtagttc atgacgcctc cattattact gtttttatat
     7741 acagtatagt tgcaatatta aaaccacaag gaatgagtgt tgattatgcg agcagactcg
     7801 cactcctgcc agtctgctgc aaaagaaagg tcaggcctta tggtggaaat aatcactcag
     7861 gcgagaaaac atgctgcctt ccccgacaga ttccagggta accagcggcc agtgcgccac
     7921 ctgtttatca cggtcgtaaa gttcaatttc ccctacccgc tgatgggcgc taattggcgc
     7981 ggtgagctct ttaccatcaa gggtatattt ggctttgata tgtggaattt cggctttcgg
     8041 tagcaccatc cagaactctt gttccgttcc cagggcgata ttttctttat cgccatacca
     8101 gatgcgttcc gtaccgacct ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg
     8161 ttgcccccaa cgcagtaatt ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc
     8221 cattaccact gcaatgagac gacgctgccc atctacagcc gaagcaatga gattaaaccc
     8281 ggcaccagaa gtatgacccg ttttcaggcc gtcaacattc atggttttat cccacaataa
     8341 cccgttacgg ttttgctggg tgataccgtt ccaggtgaga cttttctcac tgtacatatg
     8401 ataaaactcg ggctcgccgt ggatgatagc gcgagaaagc acagctaaat cataagccga
     8461 gctatgctga cctggtgcat ccagaccatg cactgtttca aaatgcgtat ccttgagatg
     8521 cagcttctcg gcatagttgt tcatcatttc aacaaactgc cgttgcccac cggcaatata
     8581 gtcagccagt gcaacacaag cgtcatttcc ggaatccaca attaaaccac ggcttaaatc
     8641 acgtaccgat acgcgatcgc cctctttcaa aaacatcagt gaagaaccga caaacactgg
     8701 attatctttc gcccacgcat cgcgccccac ggtgacaata tcgtctggcg taatgcgatg
     8761 actatcgata gcgcgatcca cgacatagcc cgtcatcagc tttgtcaggc tggcgggatt
     8821 gcgctgttga tgctcattac ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac
     8881 ccaggagccg gcatgaatct ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa
     8941 accagacgat aagttaaaaa tgaacaaaga agcagcaata ataagacggc gtttcaacag
     9001 caaaccctca ggagtttcaa atagctgttc tttttacgga aatacttatg aactggctgg
     9061 aataaagtgc aagaaaatgt gactaccctc tcatttttat ctgacatgat ctgttgccac
     9121 tcgctgtcaa attgtcgcgc taaagctgat tagcacggtg atatttgata ctctggcaga
     9181 cagcagaaac aacggattta acctaatgat gaatgacggt aagcaacaat ctaccttttt
     9241 gttttcacga ttacgcgcaa cacccctgag agccgccatg aacaagggtt tcataatttg
     9301 tgattttagt ttggtacgca atttggtaca caacacaatt ttcacttcag tgggtagtca
     9361 tcaaactcac cataacgagc atcgttgatg atgtatgtta taaccacaaa catcacatct
     9421 ggactagttc cgttttcatg agcgggtatt tgtttacgcc tccctttctg caaatcctaa
     9481 agatgagatt ttggggtttt gggatatctt ttatcctcag ttctctatcc attatgcaaa
     9541 ccagtaatga accatcacag tgtgatagtg aggcgtctgc cacgataagc gcagagaaat
     9601 gatgcaatgg tgggcggact ggcttgatga aaaggtagag tgagcgacct taaactatcg
     9661 aatagaacaa agtcttgcaa tccagtgcaa agctttgtgc gcctcagttt tgtctaagcg
     9721 ttctactgaa aacatagtaa aatcggtaac ggctggaaat cattcaatac tcgcactatc
     9781 gaaagttcgc cagccagccg tggcacgttc ttgcatacga cgtgccgcgg atcctattat
     9841 tctagtatga aatccctaac cgatttcaaa ttctccagac tgctcagatt ctcttttatc
     9901 ccacgctggc gctgataaat ctcgtcattg caatcgacct gcgctatcgc tgccgcagtt
     9961 cttccagttt ctgcatcgat aactccatct tctgagcaat aaacctcacc tatgcaatat
    10021 tttatgtcaa ttatttcaat tgcattaata tcttgaatgg attacataga gttaatgtat
    10081 ccagtactcc ctattctctt gcatataata catgttgcaa cttacatctc agcgctatga
    10141 aaaaacacca cctctctctt tatgaaattc tggatttacc aagcgctaat ttatcatttc
    10201 aatcta
//
LOCUS       DAZKFZ010000168        10189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000168 DAZKFZ010000000
VERSION     DAZKFZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            64..639
                     /gene="ssuE"
                     /locus_tag="ACHOQW_003640"
     CDS             64..639
                     /gene="ssuE"
                     /locus_tag="ACHOQW_003640"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9574650.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            632..1591
                     /gene="ssuA"
                     /locus_tag="ACHOQW_003641"
     CDS             632..1591
                     /gene="ssuA"
                     /locus_tag="ACHOQW_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP9574651.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1588..2733
                     /gene="ssuD"
                     /locus_tag="ACHOQW_003642"
     CDS             1588..2733
                     /gene="ssuD"
                     /locus_tag="ACHOQW_003642"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP9574652.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2745..3536
                     /gene="ssuC"
                     /locus_tag="ACHOQW_003643"
     CDS             2745..3536
                     /gene="ssuC"
                     /locus_tag="ACHOQW_003643"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP9574653.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3533..4300
                     /gene="ssuB"
                     /locus_tag="ACHOQW_003644"
     CDS             3533..4300
                     /gene="ssuB"
                     /locus_tag="ACHOQW_003644"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9574654.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4343..6955)
                     /gene="pepN"
                     /locus_tag="ACHOQW_003645"
     CDS             complement(4343..6955)
                     /gene="pepN"
                     /locus_tag="ACHOQW_003645"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP9574655.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7221..8423
                     /gene="pncB"
                     /locus_tag="ACHOQW_003646"
     CDS             7221..8423
                     /gene="pncB"
                     /locus_tag="ACHOQW_003646"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9574656.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8592..9992
                     /gene="asnS"
                     /locus_tag="ACHOQW_003647"
     CDS             8592..9992
                     /gene="asnS"
                     /locus_tag="ACHOQW_003647"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9574657.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2279 a         2620 c         2770 g         2520 t
ORIGIN      
        1 acagactgag cgaattgtta tcaatgttaa caaaacaaga acaattggtt ataaggagag
       61 agtatgcgtg tcatcaccct ggcgggtagt cctcgctttc cttctcgctc cagctccttg
      121 ctggaatatg cgcgggaaaa actaaatggc ctggatgttg aggtttatca ctggaatctg
      181 caaaacttcg ccgcggaaga tctgctttat gctcgtttcg atagtccggc actccatacc
      241 ttcaccgaac agttgcaaca ggctgacggg ctgattgtcg ccacgcctgt gtataaagcc
      301 gcttattccg gtgcgctgaa aaccctgctc gacctgctgc cagaacgtgc gttgcaaggc
      361 aaagtggtgc taccgctggc gacgggcggt acggtggccc atctgctggc ggtcgattat
      421 gcccttaagc cagttttaag tgcgctgaaa gcccaggaga tcctgcacgg cgtgtttgcc
      481 gatgactcac aagtaattga ttaccatcac aaaccccagt tcacgccgaa tttacaaacc
      541 cgtcttgata ccgcgctaga aactttctgg caggcattgc accgccgcga tgttcaggtt
      601 cctgatcttc attctctgcg aggtaatgcc catgcgtaac atcattaaac tggcgctggt
      661 gggattgctt agcgtctcta cgattgtggt tgctgcagaa tcctcgcctg aagcgttacg
      721 tataggctat cagaaaggca gtattggtat ggtattggca aaaagccacc agttactgga
      781 aaaacgctat ccgcaatcaa aaatctcgtg ggtggagttc cccgctggcc cgcaaatgct
      841 tgaggcgtta aacgttggca gtattgatct cggcagtacc ggggatattc cgccaatctt
      901 cgcccaggct gccggggctg atttgctgta cgtgggcgtc gagccgccga agccaaaagc
      961 cgaggtgatt ctggtggcag aaaacagccc gatcaaaacc gcagccgatc ttaaaggcca
     1021 caaagttgcg ttccagaaag gttccagttc acacaatctt ttactgcgcg cactacgcca
     1081 ggccggactt aagtttactg acatccagcc cacttatctg acgccagctg atgcccgcgc
     1141 cgcgttccag caaggtaacg ttgacgcctg ggctatctgg gatccctact actccgctgc
     1201 attattacag ggcggcgtgc gggtgttgaa agacggcacc gatctcaatc aaaccggatc
     1261 gttttatctg gcagctcgcc cctatgcaga aaaaaacggc gcttttattc agggtgtact
     1321 ggcaaccttt agtgaggccg atgcgttaac ccgcagccag cgcgagcaaa gtatcgcttt
     1381 actggcaaaa acgatgggct taccggcacc ggtgattgcc tcttacttag atcatcgccc
     1441 tcctaccacc atcaaaccgg ttaacgccga ggttgccgcc ttacagcagc aaacggcaga
     1501 tctgttttat gaaaatcgtc tggtgccgaa aaaagtcgat attcgccaac gcatctggca
     1561 gcccactcaa ctggaaggaa aacaattatg agtctgaata tgttctggtt tttaccgacc
     1621 catggtgacg ggcattatct gggaacggaa gaaggttcac gcccggttga tcacggttat
     1681 ctgcaacaaa ttgcgcaagc ggcggatcgt cttggctata ccggtgtgct gatcccaacg
     1741 gggcgctcct gcgaagatgc gtggctggtt gccgcatcga tgatcccggt gacgcagcgg
     1801 ctgaagtttc ttgtcgccct gcgtcccagc gtaacctcac ctaccgttgc cgcccgccag
     1861 gccgccacgc ttgaccgtct ctcaaatgga cgtgcgttgt ttaacctggt cacaggcagc
     1921 gatccacaag aactggcagg cgacggcgtg ttccttgatc atagtgagcg ctacgaagcc
     1981 tcggcggaat ttacccaggt ctggcggcgt ttattgctgg gggaaaccgt cgatttcaac
     2041 ggtaaacata ttcatgtgcg gggagccaaa ctgctcttcc cgccgattca acagccgtat
     2101 cctccgcttt actttggcgg atcgtcagac gtcgcccagg agctggcggc agaacaggtt
     2161 gatctctacc tcacctgggg cgaaccgccg gaactggtta aagagaaaat cgaacaagtg
     2221 cgggcgaaag ctgccgcgca tggacgcaaa attcgtttcg gcattcgtct gcatgtgatt
     2281 gttcgtgaaa ctaacgacga agcgtggcag gccgccgagc ggttaatctc gcatcttgat
     2341 gatgagacta tcgccaaagc acaagccgca ttcgcccgca cggattccgt ggggcaacag
     2401 cgaatggcag cgctacataa cggcaagcgc gacaatctgg agatcagccc caatttatgg
     2461 gcgggcgttg gcttagtgcg cggcggtgcc gggacggcgc tggtgggcga tggtcctacg
     2521 gtcgctgcgc gaatcaacga atatgccgcg cttggcatcg acagctttgt gctttcgggc
     2581 tatccgcatc tggaagaagc gtatcgggtc ggcgagttgc tgttcccaca tctggatgtc
     2641 gccattccgg aaattcccca gccgcagccg ctgaatccgc aaggcgaagc ggtggagaat
     2701 gattttatcc ctcgcagagt cgcgcaaagc taaggaagaa gataatggca acgccagtga
     2761 agaagtggtt attgcgcgtt gccccctggt ttttaccggt gggcatcgtg acggtgtggc
     2821 aactggcctc ctcggttggc tggttgtcga cgcgtatttt gccctcaccg gaaggggtgg
     2881 tgatggcgtt ctggacgctc tcagccagcg gcgaattgtg gcagcatctg gcgatcagct
     2941 cctggcgggc gctgattggt ttttcaattg gcggctcgct ggggttgatt ttgggactga
     3001 ttagcggact gtcgcgctgg ggagaaaggc tgctggatac ctcaattcag atgttgcgca
     3061 acgtgccgca tctggcgctg atcccgctgg tgattttgtg gtttggcatt gatgaatccg
     3121 caaaaatctt tctggtggcg ctcggtacgc tattccccat ttatatcaac acctggcatg
     3181 gcatccgtaa tatcgatcgc gggttggtgg agatggcgcg tagctatgga ttatccggca
     3241 taccgctgtt tatccatgtg atcctgcctg gtgccctgcc ctcaattatg gtcggcgtgc
     3301 gttttgcgtt gggcctgatg tggctgacgc tgattgttgc cgaaaccatt tctgccaatt
     3361 caggcattgg ttatctggcg atgaatgcgc gggagttttt gcaaacggac gtggtggtag
     3421 tcgctattat tctttacgcc ctgctcggca aacttgctga cgtcagcgcg cagctgttag
     3481 agcgcctctg gttgcgctgg aacccggctt atcatttgaa ggaggccacg gtatgaatac
     3541 tgctcgtctg aaccagggca caccattgtt gctcaatgca gtaagcaaac attacgcgga
     3601 aaatatcgtc ctgaaccaac tggatttaca tattccggca ggtcagtttg tggcggtggt
     3661 gggccgcagc ggtggtggca aaagtaccct gctgcgcctg ctggcaggtc tggaaacgcc
     3721 aaccgctggc gatgtgttag cgggcaccac accgctggct gaaattcagg aagacacgcg
     3781 aatgatgttt caggatgcgc gtctgctacc gtggaaatcg gtgattgata acgttgggtt
     3841 aggccttaaa ggccagtggc gcgatgccgc gcgtcgggcg ttggctgcgg taggactgga
     3901 gaatcgcgcc ggggaatggc ctgccgcact ttctggcggg cagaaacagc gagtggcgct
     3961 ggcaagagcg ttgattcatc gaccgggttt attgttgctt gatgaaccgc tcggtgcgct
     4021 ggatgcctta acgcgactcg agatgcagga tttgattgtg tcactttggc aggagcacgg
     4081 ttttaccgta ctgttggtga cgcatgatgt gagcgaagcg gtagcaatgg ctgatcgggt
     4141 gttgttaatt gaagagggaa aaattggtct ggatttgacg gtggatattc cccgtccgcg
     4201 ccggttaggg tcggtgcgat tagcggagct ggaagcggaa gtgttgcaac gggtgatgca
     4261 gcgtggtgaa tctgaaactc gcctgagaaa acaggggtaa aatccccgaa tggcggcgct
     4321 attgccgcca ttcggggatt tattaagcca gtgctttggt gatcttctcg tacagatcgc
     4381 cagatagatt ttccagccct ttcaactgtt ccagcgccgc gcgcattttc tcctgacgtt
     4441 tggcatcata acgtttcagg cgaatcagcg gttcaatcag gcgtgaagct acctgcgggt
     4501 tacggctgtt caggtcggta agcatttcca ccaggaactg gtaaccgctc ccatcttcgg
     4561 catggaacgc tgccgggttg ctgcccgcaa acgcaccaat caacgaacga atgcggttcg
     4621 ggttgctcat ggtaaatgag cgatgctgca acaggccgcg caccgtctcc agcacattcg
     4681 ccgccgggct ggtggcttgc aggataaacc atttatccat caccagacca tcctgatgcc
     4741 acttgtcgtc gtactcctgc atcagcgcgt cacggcaagg cagctgtgca gcaaccgccg
     4801 cagaaagcgc tgccagcgca tcggtcatat tgtttgcttc atggtattgc ttgctcacca
     4861 gcacgtcagc cagatgcgtt tcaccaaaag caaggaagcg caggcaggca ttacgcagag
     4921 tgcgttttgc aatatcttca tgctcaacac ggtactcgct ctggtaattc gcgttgtaaa
     4981 tagccagtag ctcatccgcc agttcagtcg ccagagtacg agtgagtgct tcgcgtactt
     5041 cggcaatagc aatcgggtcg atgatatcga acagttcagc catttcattg acagaaggca
     5101 gcgtcaggat ttctgccgcc agcgccggat caatcttctc atcgagcagc accgcgcgga
     5161 aagcgtcagc cacatgtacc ggcagagaca gcggctgccc ttgctgatga cgggcgacgt
     5221 tcagcttgat gtaggttgcc agcaaacttt gcgccgcatc ccaacgggaa aaatcattac
     5281 gcgcatgacg catcaggaag gtcagttgct gatcgctcca cttatattcc agtttcactg
     5341 gcgcagagaa ttcgcacagc agcgcaggca ccggctggaa gtagacatta tcaaaaacaa
     5401 aggtctgttc cgcctgggtg acgttcagca cggaattcac cggatgaccg cctttctgca
     5461 acgggatcac tttgccttcg ttgtcataca attcgatggc aaacggaata tgcagcggct
     5521 gtttttctgc ttgatccggc gtggctggcg tgcgctggct gatggtcagg gtgtactgct
     5581 cggtttccgg attgtagtcg tctttgacgg tcacaaccgg cgtaccggac tggctgtacc
     5641 aacggcggaa atgggagaga tcgacattcg acgcatcttc catcgcctga acgaaatcgt
     5701 cgcaggtcgc tgcactgcca tcatggcgct cgaaatagag ctgcatccct ttctggaagt
     5761 tttcttcgcc aagcagagtg tggatcatac gaatcacttc cgcgcccttc tcgtaaacgg
     5821 tcagggtgta gaaattgttc atctcaatga ccatgtccgg gcggattggg tgcgccatcg
     5881 ggctggcgtc ttccgcaaac tgcaatccgc gcatggtgcg cacattattg atacggttta
     5941 ccgcgcggga accaaggtca gagctgaatt cctgatcgcg gaagacggtt aaaccttctt
     6001 tcaggctgag ctggaaccag tcgcggcagg tcactcggtt accggtccag ttatggaaat
     6061 attcatggcc gataacgcgt tcaatatcga ggtaatcttt gtcggtggcg gtatcggtgc
     6121 gggccagcac atatttggag ttaaagatat tcagaccctt attctccatt gcgcccatat
     6181 tgaagaaatc caccgcgacg atcatataga tgtcgaggtc atactccagg ccaaagcgtt
     6241 cttcatccca tttcatggag tttttcagcg aggtcatcgc ccacggcgcg cgatcaaggt
     6301 tgccgcgatc gacgtacagc tccagtgcta cttcgcgacc agaacgcgtg gtaaaggtat
     6361 cgcgcagtac atcaaagtcg cctgccacca gcgcaaacag gtagcacggt ttcgggaacg
     6421 ggtcctgcca ctgtacccaa tggcgtccgt tttccagttc gccttgcgca acgcggttac
     6481 cgttggaaag caggaaggga tatttggttt tatcggcaat aattttggtg gtaaaacgcg
     6541 ccagcacgtc cgggcggtcg agataatacg taatatggcg gaaaccttcg gcttcacact
     6601 gggtgcaaag cgcatcgcct gactgataaa gcccttccag cgccgtattc gccgccgggc
     6661 ttatttcatt aatgatctta agcgtaaaac gctccggcaa attactgatg accagtgcgc
     6721 cctcttcttc tttccaggcg gtccacggct catcattaat atgaacagaa accagtttga
     6781 ggtcttcgcc gttgagacaa agcggagcat ctgatgcacc atgacggaca gcctggctga
     6841 ccgcggtaac gaccgtcttt tgcgcgtcga ggtcaaaggt caagtcaata tcagtaatct
     6901 ggtaatccgg cgcacgataa tcgtgacggt atttggcttg tggctgttga gtcataaata
     6961 acctttagca tcttttatag agtctggtgt tcagtctatt cctgttgcgt aaatcgcgct
     7021 atgcagaatc ttcatctttt caggtacaaa cgcctttatt gctacatttt tataacatac
     7081 accgcgtaat gccatcgacc agaaaggtgg catatggtgt gatcggggtt caataaattg
     7141 cgaaacaagg tatactccag cagttcctga agatgtttat tgtactaaac gctcctgtac
     7201 gaggacgcta ctgcgcacct atgacacaat tcgcttctcc tgttctgcac tcgttgctgg
     7261 atacagatgc ttataagttg catatgcagc aagccgtgtt tcatcattat tacgatgtgc
     7321 atgtcgcggc ggagtttcgt tgccgaggtg acgatctgct gggtatttat gccgatgcta
     7381 ttcgtgaaca ggttcaggcg atgcagcacc tgcgcctgca ggatgatgaa tatcagtggc
     7441 tttctgccct gcctttcttt aaggccgact atcttaactg gttacgcgag ttccgcttta
     7501 acccggaaca agtcaccgtg tccaacgata atggcaagct ggatattcgt ttaagcggcc
     7561 cgtggcgtga agtcatcctc tgggaggttc ctttgctggc ggttatcagt gaaatggtac
     7621 atcgctatcg ctcaccgcag gccgacgttg cgcaagccct cgacacgctg gaaagcaaat
     7681 tagtcgactt ctcggcgtta accgctggtc ttgatatgtc gcgcttccat ctgatggatt
     7741 ttggcacccg tcgccgtttt tctcgcgaag tacaagaaac catcgttaag cgtctgcaac
     7801 aggaatcctg gttcgtgggc accagcaact acgatctggc gcgtcggctt tccctcacgc
     7861 cgatgggaac acaggcacac gaatggttcc aggcacatca gcaaatcagc ccggatctag
     7921 ccaacagcca gcgagctgca cttgctgcct ggctggaaga gtatcccgac caacttggca
     7981 ttgcattaac cgactgcatc actatggatg ctttcctgcg tgatttcggt gtcgagttcg
     8041 ctagtcggta tcagggcctg cgtcatgact ctggcgaccc ggttgaatgg ggtgaaaaag
     8101 ccattgcaca ttatgaaaag ctggaaattg atccacagag taaaacgctg gttttctctg
     8161 acaatctgga tttacgcaaa gcggttgagc tataccgcca cttctcttcc cgcgtgcaat
     8221 taagttttgg tattgggact cgcctgacct gcgatatccc ccaggtaaaa cccctgaata
     8281 ttgtgattaa gttggtagag tgtaacggta aaccggtggc gaaactttct gacagccctg
     8341 gcaaaactat ctgccatgat aaagcgtttg ttcgggcgct gcgcaaagcg ttcgaccttc
     8401 cgcatattaa aaaagccagt taatatcatc agggagctaa tcggctccct ttttttacct
     8461 ttaattccga aatctttcgc tgcatttgcg aattctgctt gtctgattgc agatgccagg
     8521 taacataggt atccccccat ttacgggtgg acaagtgttt attttttccg actattaaca
     8581 gagagaatat tatgagcgtt gtgcctgtag ccgacgtact ccagggccgc gtagccgttg
     8641 acagcgaagt caccgtacgc ggatgggtac gtacccgccg agattcaaaa gctggcatct
     8701 ccttcctcgc cgtttatgac ggttcctgct ttgatcctgt acaggctgtc atcaataatt
     8761 ctctgcccaa ttacaatgaa gacgtcctgc gtctgaccac cggctgctcg gtcattgtga
     8821 cgggtaaagt cgtggcgtcg ccgggccagg ggcaacaatt tgaaattcag accagcaagg
     8881 ttgaagttgc tggttgggtt gaagatccag acacttaccc gatggcggca aaacgccaca
     8941 gcattgagta tctgcgtgaa gtcgctcacc tgcgtccgcg cacaaacctg attggtgccg
     9001 tcgcgcgcgt tcgccatacg ctggcgcagg cgctgcatcg cttctttaac gagcagggat
     9061 tcttctgggt ttcaacgcca ctgattactg cgtctgatac cgaaggggct ggcgaaatgt
     9121 tccgcgtttc tacgctggat ctggaaaatc tgccgcgtaa cgatcagggc aaagtggatt
     9181 tcgacaaaga cttctttggt aaagagtctt tcctgaccgt atctggccag ttgaacggcg
     9241 aaacctacgc ttgcgcatta tccaaaattt ataccttcgg cccgactttc cgtgctgaaa
     9301 actccaacac cagccgtcac ctggcggaat tctggatgct ggagccggaa gtggcgtttg
     9361 ctaacctgaa cgatatcgcg ggtctggctg aagccatgct gaaatatgtc ttcaaagcgg
     9421 ttctcgaaga acgcgctgac gacatgaaat tcttcgctga acgcgtagat aaagatgccg
     9481 tttcacgtct ggaacgcttt attgaggccg atttcgcgca ggtggattac accgaagcag
     9541 taaccattct cgaaaactgc ggcaggaagt ttgaaaaccc ggtttactgg ggcgtcgatc
     9601 tctcttctga gcatgagcgt tatctggcgg aagaacactt taaagcaccg gtagtggtta
     9661 aaaactatcc gaaagatatt aaagcgttct atatgcgcct caacgaagac ggtaaaaccg
     9721 ttgcggctat ggatgttctg gctccgggca tcggtgagat cattggtggc tcccagcgtg
     9781 aagagcgtct ggacgtgctg gacgagcgta tgctggaaat gggcctgaac aaagaagatt
     9841 actggtggta tcgcgatctg cgtcgctacg gtactgttcc gcattccggt ttcggtcttg
     9901 gttttgaacg cctgattgct tacgtaactg gtgtgcaaaa cgtgcgtgat gtgattccgt
     9961 tcccacgaac tccgcgtaac gccagcttct aatttagcgt ctacaagagc cagcacccgc
    10021 tggctctttt ttatcctttc tgtcaagtta tctgtttgtt aagtcaatca atctatttgc
    10081 aaccccgcca taacgatcat cctgttacgg aatattacat tgcaacattt acgcacaaaa
    10141 aataacccgc attcttattg cggattagtt ttttcttagc tgatagcac
//
LOCUS       DAZKFZ010000169        10155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000169 DAZKFZ010000000
VERSION     DAZKFZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            58..261
                     /locus_tag="ACHOQW_003648"
                     /pseudo
     CDS             58..261
                     /locus_tag="ACHOQW_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            260..781
                     /gene="prfH"
                     /locus_tag="ACHOQW_003649"
                     /pseudo
     CDS             260..781
                     /gene="prfH"
                     /locus_tag="ACHOQW_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(838..2295)
                     /gene="pepD"
                     /locus_tag="ACHOQW_003650"
     CDS             complement(838..2295)
                     /gene="pepD"
                     /locus_tag="ACHOQW_003650"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9574658.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2556..3014
                     /gene="gpt"
                     /locus_tag="ACHOQW_003651"
     CDS             2556..3014
                     /gene="gpt"
                     /locus_tag="ACHOQW_003651"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9574659.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3106..4350
                     /gene="frsA"
                     /locus_tag="ACHOQW_003652"
     CDS             3106..4350
                     /gene="frsA"
                     /locus_tag="ACHOQW_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9574660.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4408..4809
                     /gene="crl"
                     /locus_tag="ACHOQW_003653"
     CDS             4408..4809
                     /gene="crl"
                     /locus_tag="ACHOQW_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9574661.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4848..5903)
                     /gene="phoE"
                     /locus_tag="ACHOQW_003654"
     CDS             complement(4848..5903)
                     /gene="phoE"
                     /locus_tag="ACHOQW_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9574662.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6191..7294
                     /gene="proB"
                     /locus_tag="ACHOQW_003655"
     CDS             6191..7294
                     /gene="proB"
                     /locus_tag="ACHOQW_003655"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9574663.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7306..8559
                     /gene="proA"
                     /locus_tag="ACHOQW_003656"
     CDS             7306..8559
                     /gene="proA"
                     /locus_tag="ACHOQW_003656"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9574664.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            8674..8749
                     /locus_tag="ACHOQW_003657"
     tRNA            8674..8749
                     /locus_tag="ACHOQW_003657"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8707..8709,aa:Thr,seq:cgt)
     gene            complement(8764..9684)
                     /locus_tag="ACHOQW_003658"
                     /pseudo
     CDS             complement(8764..9684)
                     /locus_tag="ACHOQW_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(9629..9874)
                     /locus_tag="ACHOQW_003659"
     CDS             complement(9629..9874)
                     /locus_tag="ACHOQW_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP9574665.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
BASE COUNT         2407 a         2511 c         2621 g         2616 t
ORIGIN      
        1 cgaacgcagt cgcagtacat gcaacttgaa gtatgacgag tataaatgga atggtacatg
       61 ggcaaatata ttcgtccctt atccgatgcg gtatttacca tcgcatctga tgacctgtgg
      121 atcgagagtt tagcgatcca acaattacac accacggcaa atttacccaa catgcagcgc
      181 gtagtgggga tgccagattt acaccccgga cgcggctacc cgattggcgc agcgttcttc
      241 tctgttggtc gtttttaccc gacaagacgt cgcggtaacg gtgctggaaa cagaaacggg
      301 ccgctactct gacacgctgc gttcggcgct gatttctctg gatggcgaca acgcatgggc
      361 gttaagcgaa agttggtgcg gcactattca gtggatttgt ctgagtccgt atcggcctca
      421 tcatgggcgc aaaaactggt ttctgggcat tgggcgtttt accgctgatg agcaggaaca
      481 atcggatgca atccgttatg agacgctgcg tccgtcgggg ccgggcggtc aacatgtcaa
      541 taaaaccgac tcggcggtac gcgccacgca tctggcaacc gggattagcg tgaaggttca
      601 gtcagaacgc agccagcatg ctaacaaacg gctggcgcga ttgctgattg cctggaagct
      661 ggagcagcag caacaggaaa atagcgcagt gctgaaatcg cagcggcgaa tgttccatca
      721 ccagattgaa cgtggcaacc cgcgacgaac gttcacaggg atggctttta tcgaaggata
      781 atgaaggatg aaactgccgg aaggcgatta aacgccatcc ggcagcaaat caaataatta
      841 cttcgccgga atttctttca gcagttcagt cagcagtgtc cagtaatgac ctacgctttc
      901 gatgtgaact tgctcatccg gagagtgtgg accggtgatt gttggcccga tagaaaccat
      961 gtccatttcc ggatacggtt ttttgaacag accacattcc aggcccgcgt ggataatctg
     1021 gatgttcggc gtcttgttga acaggcgctg ataggtttca cgtaccagat gcatcaccgg
     1081 agaattagcg tccggctgcc agccaggata tgcgcctttc gcttcggttt tcgcgccagc
     1141 cagtttaccc agcgaatcca gcatgctcac cacgtagtct ttaccgctgt cgatcagaga
     1201 acggatcaga cagtgaattt ctacgttatt gtcagtcatg gtcaccacac cgacgttcag
     1261 ggaggtttcg accacacctt ttgccacgtc ggagttgcgg atcacaccgt tcggggtggc
     1321 gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc
     1381 tacagagtcc agcaacaagg ccagattttt ctctttctct gccagctcgt ttttcaggat
     1441 ctcctgatag gtattcacca gagatttcag ggcgtcgact ttatcagctg cgacagcaat
     1501 ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga aatcaataag
     1561 acgcaggtcc agttcttctg catgacccgc caggaagcgc accagcagtt tgttggcatt
     1621 acccaggcca acgtggattt ccccgccgga gtgaccgcct ttcagacctt ttaaggttaa
     1681 cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa
     1741 gtcgataccc cccgcgcaac ccatgtagat ttcaccttct tcttcggagt cggtgttaat
     1801 cagaatatca gcctgcaacc agttgctctg taagccgaac gcaccgtcca taccggcttc
     1861 ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc acgttttcgt cagccagaac
     1921 cgccagtgca gaggccatac caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt
     1981 aacccattcg ccatcaatat atggctggat aggatctttc gtgaagtcat gcacggtgtc
     2041 gttatttttc tgcggcacca tatcgaggtg ggcctgcaag acgaccggtt tacgattttc
     2101 catacctgcg gtagcaggtt tacgaatcag gatattacct acctgatcgc gttcgacatg
     2161 gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt catgatagga
     2221 cgggtgagga atagaacaga ttttggcaaa aatatcccac agcggctgtg gagataattg
     2281 agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta ttgcaggtca
     2341 cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt ttgagaatac
     2401 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     2461 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     2521 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     2581 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     2641 ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg
     2701 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     2761 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     2821 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     2881 ttgtcaccat ctttgcaaaa ccggctggtc gtccgctggt tgatgattat gttgttgata
     2941 tcccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     3001 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     3061 ggcgagttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     3121 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     3181 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     3241 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     3301 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     3361 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cacccaggcg
     3421 atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc aactcagtgg tcgtcactgg
     3481 ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg
     3541 gccgagcaag cgcaggcttt gtcaaaccgc gcctatgaag aggccgctca gcgtctaccg
     3601 ggcacgatgc gacagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg
     3661 catatgccga aaggcgatgg cccgttcccg acggtattaa tgtgtggtgg tctggatgcg
     3721 atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg
     3781 ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc
     3841 tgcctgttgc atcagcgcgt cttaaaggct ctgcctaacg taccgtgggt ggatcacact
     3901 cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatatctt
     3961 gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctgttgagt
     4021 gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc gagtcgtttg
     4081 gggatgcatg atgcttccga cgaagcgttg cgcgtggagc tgaatcgcta ttcattaaaa
     4141 gtgcaaggat tgctgggacg tcgatgccca acgccaatgt tatcaggcta ctggaagaac
     4201 gatccgttca gcccggaaga ggactcacgc ttaatcacct catcatctgc tgacggtaaa
     4261 ttattagaga tcccatttaa cccggtgtat cggaattttg acaaaggtct tcaggaaatc
     4321 accgactgga tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca atttggtaaa
     4381 acagttgcat cacaacagga gatagcaatg acgttaccga gtggacaccc gaagagcaga
     4441 ttgatcaaaa aatttaccgt actaggcccg tatattcgtg aaggtaagtg cgaagataat
     4501 cgattctttt tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt
     4561 gagttctggg gctggtggat ggagcttgaa gcgcaggaat cccgttttac ctacagttac
     4621 cagtttggtc tgttcgataa agcaggcgac tggaagagtg ttccggtaaa agatactgaa
     4681 gtggttgaac gactggagca caccctgcgt gagtttcacg agaagctgcg tgaactgctg
     4741 acgacgctga atctgaagtt ggaaccggcg gatgattttc gtgacgagcc ggtgaagtta
     4801 acggcgtgag tgaaatgtgc cggatgcatc acatccggca ataatcatta aaactgatag
     4861 gtcatgccaa ccgcgacaat atcatcatta ttaatattca atttgttatc gctatccagt
     4921 tggttgattt tataatcaac aaacgctgac atatttttgt tgaaataata tgtagccccg
     4981 acatcgatat aattgaccag atcttcatca ccgatacctt caatatcttt ccctttcgat
     5041 aagacataac ccagcgatgg acgcagacca aagtcaaact ggtattgagc gaccgcttca
     5101 aagttctgtg tcttattggc aaagccgcca gttattggcg tcattttgcg tgtttcagaa
     5161 taaaaagttg ccagataaat attattggca tcgtatttca gacctgtagc ccaagcttct
     5221 gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt tggtgcgatc tgagttggta
     5281 taggccccac taatggcgaa atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag
     5341 ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt
     5401 aagttcaggc catcgataac gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg
     5461 gcgcgtttgg tcataaagtt gtcggtctgc gccgaggagt cgccaccaaa ttccgggaac
     5521 atatcggtcc aggcttccac gtcatacaac gcgcccaggt tacggccata gtcgaaagaa
     5581 cccaaatctt tatacttcaa tccggcaaaa gcgagacgcg ttttttgctg tgcagtatca
     5641 ctctccgctt tatttccggc aaactccgct tcccaacgac cataaccagt cagttgatcg
     5701 ttaatttgtg tttcgccttt aaaaccaaaa cggatataac tctggtcgcc atctttactg
     5761 tcgttatcac tcatataatg catggcttta actttgccat agacatccag tttattaccg
     5821 tctttgttat atatttctgc ggcctgtacg gatgcagatg ccacaatgcc catcaccact
     5881 aatgccagag tgctcttttt cattttcatt cctgatttta attaacgcgc gaatattctg
     5941 cgggagagcc ccgttgaaaa caggaaagtt tttaacctga gattgttaaa gatatattac
     6001 agattaatga tattcttaaa atgtggtaat ttattaaatc tgtaataaaa gcgtaaacaa
     6061 ctgccgctag gcttgctgat cccgcgcaac aaaacgccat gctttgctcg cagatggttg
     6121 gcaaccgacg acagtcctgc taaaacgttc gtttgatatc atttttccta aaattgaatg
     6181 gcagagaatc atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac
     6241 aggcggatcg cgccgcctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca
     6301 gttacatgcc gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg
     6361 tgagcacctg ggttacccgg aactgccagc gactatcgcc tcgaaacaac tgctggcggc
     6421 ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt atggcattca
     6481 cgtcgggcaa atgctgctga ctcgtgctga tatggaagac cgtgaacgct tcctgaacgc
     6541 ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga
     6601 tgctgtcgct acggcagaga ttaaagtcgg cgataacgac aacctttctg cactggcggc
     6661 gattctggcg ggtgccgata aactgttgtt actgaccgat caaaaaggtt tgtataccgc
     6721 tgacccgcgc agcaatccgc aggcagaact gattaaagat gtttacggca ttgatgacgc
     6781 actgcgcgcg attgctggtg acagcgtttc aggcctcgga actggcggca tgagtaccaa
     6841 attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag
     6901 caagccgggc gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc
     6961 ccaggcgact ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc ctgcgggtga
     7021 aatcacggta gatgaagggg caaccgccgc cattcttgaa cgcggcagct ccctgttgcc
     7081 gaaaggcatt aaaagcgtga ctggcaactt ctcgcgtggt gaagtcatcc gcatttgtaa
     7141 cctcgaaggt cgcgatatcg cccacggcgt cagtcgttac aacagcgatg cattacgccg
     7201 tattgccgga caccactcgc aagaaattga tgcaatactg ggatatgaat acggcccggt
     7261 tgccgttcac cgtgatgaca tgatcacccg ttaaggagca ggctgatgct ggaacaaatg
     7321 ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat
     7381 cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat cctcaacgct
     7441 aacgcccagg atgttgctga cgcgcgagcc aatggcctta gcgaagcgat gcttgaccgt
     7501 ctggcactga cgcccgcacg gctgaaaggc attgccgacg atgtacgtca ggtgtgcaac
     7561 ctcgccgatc cagtggggca ggtaatcgat ggcggcgtac tggacagcgg cctgcgtctt
     7621 gagcgtcgtc gcgtaccgct gggggttatt ggcgtgattt atgaagcgcg cccgaacgtg
     7681 acggttgatg tcgcttcgct gtgcctgaaa accggtaacg cggtgatcct gcgtggtggc
     7741 aaagaaacct gtcgcactaa cgcggcaacg gtggtggtga ttcaggacgc cctgaaatcc
     7801 tgtggcttac cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt
     7861 gaaatgctgc gtatggataa atacatcgac atgctgatcc cgcgcggcgg ggctggtttg
     7921 cataaactgt gccgcgagca gtcgacgatc ccggtgatca caggtggtat aggcgtatgc
     7981 catatttatg ttgatgaaag tgcagagatt gctgaagccc tgaaagtaat cgtcaatgcg
     8041 aaaactcagc gtccgagcac atgtaatacg gtagaaacgt tgctggtgaa taaaaacatc
     8101 gcctatagct tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt gacgttacac
     8161 gcagatgcat ctgcgctggc gcagttgcag acaggccctg cgaaggtggt ggcggttaaa
     8221 gccgaagagt atgacgatga gtttctgtca ttagatttga acgtcaaaat cgtcagcgat
     8281 cttgacgatg ccatcgccca tattcgtgaa cacggcacgc aacactccga tgcgatcctg
     8341 acccgcgata tgcgcaacgc ccagcgtttt gttaacgaag tggattcgtc cgctgtttac
     8401 gttaacgcct ctacgcgttt taccgacggc ggccagtttg gactgggtgc ggaagtggcg
     8461 gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc tggaagcact gaccacttac
     8521 aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa
     8581 gtagccgttt gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac
     8641 cgtgattcac gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag
     8701 cgcattcgta atgcgaaggt cgtaggttcg actcctatta tcggcaccat ttaaatcaat
     8761 aagttacaca tcattagtac cttccttatt ttttgactgg gacaaatttg ggaccgatgg
     8821 gttcaggatc gagtctattt gccgtgcgtg ttcggtaagg tgattaggtg caaggtgagc
     8881 atatcgacga accatttcga tagactccca gcctcccatt tcctgtaaca ctgacaacgg
     8941 gactccggct tgaaccagcc aacttgccca ggtgtgtctc aagtcgtgaa atctgaaatc
     9001 atcaatacca gcccgtctca gcgccgcttt ccaggctgtg tttgcgtcat accgcatctt
     9061 ccttactgtt ggcgctttcg ttccgtctgg tttggtacag ctttccttgt acacaaatac
     9121 ccaacggtga tgattaccga tttgtttttt caatacgcga catgcagtat cattcagcgc
     9181 aacgccaatt gcgcggtttg atttactctc ttccgggttt atccatgcca cccggcgctg
     9241 catatctatt tgttgccatt caaggttgat gatgttcgag cgtcttaagc ctgttgccag
     9301 tgcaaattca acaacagact ttaatggctc cggacattca tcaatcagcc tttgtgcttc
     9361 atggggctcc agccagcgga tccgtttatt ctttggttga ggcactttaa taattggtgc
     9421 cttatcgagc attttccatt cacgctctgc ggctcttagc agggccttta taaatgaaag
     9481 atgcgtagcc ttcgttgcaa cggacgctgg ttttggcgtg tattctggaa caggtttccc
     9541 tttttttctg catgcttctg ccctgagttt ccagttttcc tcatgacgcc ggttcgtcat
     9601 tttctgcatt gctgaataaa tttttgattc agtaatgtct cttagttgca ttcctgcgaa
     9661 atgttgaagc cagaatccga tctgctttcc attgagcctg tttctctgcg cgacgttcgc
     9721 ggcggcgtgt ttgtgcatcc atctggattc tcctgttagt tagctttggt tgtgtgtggg
     9781 agtcgtagtc ctgaacgaaa acaccccgca atggcacatt ggctgctaat ccggattcgc
     9841 acttccggcc aatgcttcgt ttcgtatcac acacaccaaa gccttctgct ttgaatgctg
     9901 cccttcttca gggcttaatt tttaagagca tcaccttcat ggtggttagt gcgtcctgct
     9961 gatgtgcttg gtatcaccgc tagtggtatt tgtgtcaaca tcgccagaga taatttatca
    10021 ccgcagatgg ttatctgtat gttttttata tagatttatt ttttgcaggg ttgtgtggct
    10081 tgggaggtga tcgagagatc agaattgcgt tgtttagtga gttgcaccta ttaattttaa
    10141 aataaataca attgg
//
LOCUS       DAZKFZ010000170        10076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000170 DAZKFZ010000000
VERSION     DAZKFZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            6..81
                     /locus_tag="ACHOQW_003660"
     tRNA            6..81
                     /locus_tag="ACHOQW_003660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHOQW_003661"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHOQW_003661"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHOQW_003662"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHOQW_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9574666.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHOQW_003663"
     CDS             complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHOQW_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9574667.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHOQW_003664"
     CDS             complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHOQW_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9574668.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="ACHOQW_003665"
     CDS             complement(3595..4620)
                     /locus_tag="ACHOQW_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9574669.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="ACHOQW_003666"
     CDS             complement(5051..5272)
                     /locus_tag="ACHOQW_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574670.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHOQW_003667"
                     /pseudo
     CDS             complement(5525..8674)
                     /gene="acrF"
                     /locus_tag="ACHOQW_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHOQW_003668"
     CDS             complement(8686..9843)
                     /gene="acrE"
                     /locus_tag="ACHOQW_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP9574671.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2683 a         2593 c         2433 g         2367 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcttgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc acctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaaa
     1681 ggcagcatga ccgaagacat aaacagaacg gtaatcagcg gcacgccgcg ccagaattcg
     1741 ataaaaatga ccgataagat gcgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcactgcc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatgc caagttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc cgggcgcgag ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttccacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc ccgcgcggat aatctcggcg
     2701 ataaatgcag aggtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagccttgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccgatgatca acactgcggc gatgggccag
     2881 gtgcggcgca gctgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcgcg aaccgtaggg
     3481 ttggcaaaag agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg
     3601 cacgggcggt gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt
     3841 ctgggaattg atgcccatct cttcagcatt tagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac
     3961 ctccggtaag acaatccatt cagccgggtt gcttaatttg atgcgcaggg catacagttg
     4021 ggattgatcc gaagccagcg tatcgcagcg accggattcc agtgccttcg ctgattcgtc
     4081 agaacgatcg aaagtcactg gtgtgtactt catattgttt gccttgaaat agtcggcgac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 agtaaatgcc attcccatcc cggcatcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccact tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa catcaacatc
     4441 aatacctgaa aacttaccgt ctgcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttgcctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc catattttgg cgcaaaacat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgggaa gtgcgaaagt taaattgatg cgttatttcg
     4921 aaagcagcga aataacacga tataacatgc taaatcagca gagaattagc cagcagaata
     4981 tagcaatatt ttatcttatg taaagatact gtatcgttaa acagagcgcg aaactccgcg
     5041 ccctgttcag ttatttcgac agagcgatga tccccatcgt caggccagca acagccgctg
     5101 ccccgccagc aatagcccca gccgtttccc agttatcctg agtcgtgcct ttcatacagg
     5161 tattggtgtg aaccagacga ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg cttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa atcttgcgga atgaataatg acaaattatg tgaattgtaa
     5401 aaatagtgaa gggagcggga gctatagaaa gcttttaaag agtgagcaaa agaatatctt
     5461 attaattttt atgggtttca ctggaaaaca taaaggcacc cgaaagcgcc tttatgtttc
     5521 agatttatcc tttaaagcaa cggcggatca ccacaaagaa caccggtacg aagaagattg
     5581 ccagcaacgt tgcagagacc attcctccca ttaccccgat acccactgcg ttctgcgcgc
     5641 cactgccggc accgttactg atagccagcg gtaatacgcc gagaataaag gcgagagagg
     5701 tcatcaggat aggacgcaga cgcatacgta ctgccattag tgtcgcttca acaacacctt
     5761 taccctcttt ctccatgaga tctttagcga actcaacaat caaaatagcg tttttggccg
     5821 acaagccaat tgtcgttagc aagcctacca taaagtagac gtcatttttt tgattaaaga
     5881 gtgtcgccgc cagcagcacg ccgacaatcc ctaacggcac gaccaacata accgagacag
     5941 gaattgacca gctttcatag agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg
     6001 ctaccagagc gggagcctgg tttcccgata agcgttcctg atacgacata cccgtccagt
     6061 cataaccaat gcccgcaggt aattttgacg ctaggttttc catcaacgcc atggcatcgc
     6121 cggaactggt tcctggcgcg gcttccccct gaatctccat tgacggcaga ccgttgtagc
     6181 gttccagtcg cggagagcca tacacccaat gtgaagtggt aaaggcagag aatggcacca
     6241 tttcgccgtt ggtgctgcgg acataaagtt tatcgacatc ttctggcagc atacggaatt
     6301 tggcatccgc ctgaacatac acctttttca cgcgaccacg gtcgatgaag tcgttaacgt
     6361 aagtcccacc caacgccgtt gaaatggtct gattgatgtc agaaagtgaa acacctaatg
     6421 cctgcgcctt ttcctggtca acttccagtt taaactgcgc ggtgtcttcc aggccattgg
     6481 gacgcacgct gactaagctg gcaggatgtt gcgccgccat accaagcaac tggttacggg
     6541 cctgggttag ggcatcgtga cccagcccag ccttatcaat taactcaaag tcgaaacccg
     6601 ttgccgtgcc cagttcaaca atggctggca tattgaatgg aatgacaaaa ccgtcgcgga
     6661 tcttgcccaa ctccattttg gcacgatgga ttaccgcttc cgcactgttt tcatcaccat
     6721 tacgctcttc ccacggtttc agactgacga aggccatacc tgtagattca attggtcaac
     6781 gcaacagtta tgtgaaaaca tggggttgcg gaggtgcctg gccgctgaag ctaaagccgt
     6841 taaccgtaaa gacactttca acgtttgctt tctcgttctt cagatagtaa tccgtcactt
     6901 gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc gggtaactga atcatggtca
     6961 gaaagacacc ctgatcctct tcaggtaaga aggaagacgg aagacgtaaa aacaacacca
     7021 ccattcctgc aacaatcagc gcatagatca gtaaatatcg tcctgtggaa ccgaggattt
     7081 tgccgacgct gttggtgtag tggttaacgc tatgatcgaa ggtggtatta aaccagccga
     7141 agaaaccacc cttattttcg tgatgctcag ctgaggtggg tttgagcagt gttgcacata
     7201 acgcaggggt aagaatcaat gccaccagaa cagaaagcgc cattgccgaa acgatggtga
     7261 tagagaactg acgataaatt gccccagtag aaccgccgaa gaatgccatc ggaataaata
     7321 ccgctgacag caccatcgcg atacccacca gtgcgccctg tatttgcgac attgattttt
     7381 ccgtcgcttc ttttggcggg agcttatcct ccatcatcac gcgctcgacg ttctccacca
     7441 ccactatcgc atcatcgacg agcagcccga tggcaagcac catcccgaac atcgttagtg
     7501 tgttgatgga gtaaccaaaa gcggcgagga tggcaaacgt ccctaacaac accacgggta
     7561 ccgcaatggt ggggatcagc gttgctcgca tattctgcaa gaacagatac atcaccagga
     7621 acaccagcat aatagcttcg aacagtgttt ttaccacttc gtgaatagaa agctggacga
     7681 atggcgtggt gtcataaggg tagagaacct tcattccctg cgggaagaat ggctgtaatt
     7741 ccgccagttt tgccttaatg gctttcgcgg tatcgagagc attcgcgccg gttgccagct
     7801 taatacccag gcccgccgcc ggttttccgt tgatgcgagc gataacgtta tagttttcac
     7861 cgccaagttc aacccgtgcg acatctttca gacgcaccac cgagccgtca ctgtttacgc
     7921 gcagggtcac tttgccgaat tcttccggat tttttaaccg cgtctgagca ataatcgagg
     7981 cattcaactg ttgccctggt aacgctggcg ttccgcccaa ctgtccggca gcgatctgag
     8041 cgttctgtac cttcaactgg ttaatcacat caaccggtgt cagtttatat ttgtttagca
     8101 gatcggcgtc cagccagata cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga
     8161 cgctattcag acggctaagc gtatctttaa cgttagaggc cacatagtcc gagatatcgt
     8221 cctgtgtggt gtctgggtta tcagagacaa agcctgccac catcaaatag ctgctactgg
     8281 acttttcaac actgatcccc tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct
     8341 ggagtttgtt ctgcacctgc acttgcgcga tatcaggatc ggtcccggac tggaaggtaa
     8401 gggtaattgt cacgctaccg gcggaatcgc tggtggagga catatacatc aggttatcga
     8461 taccgttcat attctgttcg ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag
     8521 cgcccggata gtttgctgaa acagaaaccg caggcggtgc aatggttgga tactgagcga
     8581 cgggcaattg taggatcgcc agtgcgcccg ccatcatcag aataatggcc agcacccatg
     8641 caaatatcgg tcgtcgaata aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt
     8701 atctgcgggg gtatcggtag tggctttaac ctgctctccc ggacgcgctt tttgcaggcc
     8761 gctgacaatg acttgatcgc cagatttcag accttcgcta atcaaccatt tatcgccaat
     8821 cgcctgactg gcaacgacag ggcgcacttc cacctgactt ttatcgttaa caatcagcac
     8881 ggttgcatca ccacgcggtg tgcggctaac gccttgttgc gggatgagaa tggcgtcagg
     8941 ttggacgcct tcatcaatcc gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg
     9001 gttagggaag acagcacgta gggttatgga gccggtgctt tcatcaacgg tcacatcgga
     9061 gaattgcagc gtacctttca ggggataggt ttgaccgttt tccatgacca actctacgtt
     9121 gctggtggcg ttttccttat gcaaatttcc ttgctctacg gactgcttca gcctcataaa
     9181 atcgttgctg gattgggtca catcaacata gataggatcg agctgctgga cagttgccag
     9241 ttcagtcgtt tgcccattag tgacaagagc gccttcggtc acagtcgatt tgccgatacg
     9301 tccgctaatt ggcgcagtca ctttggtata agcaagattg atgcgagcgc tttcgactgt
     9361 ggcttttgcg gcaatcacgg cggcatcggc ctggcgagca tcagcaatgg cctggtcgta
     9421 ctcctgctgg ctgatgtatt tcgtacccac gagcggaacg taacgtttta ccgtcaaatg
     9481 cgcgatggcg gcggcggctt cacttttcgc cagttcgcct ttcgcgctgt cataatttgc
     9541 ctgataggtc gcgggatcga tctggtacag ggactggcct gcttgcacat cgctgccttc
     9601 agtgaaattg cgattcagta cgatcccgct aacctgtggg cgaacttcgg ctatacgata
     9661 agcattggtg cggcctggta attcagtctt aacttctaac ggggccgttt ttacaatatg
     9721 aacggtaacc tgcggttcac cgacgtgagc tttctcttct cccttatcgt tacaaccggc
     9781 gattaaagcc gcggagatca gaataaagga gggcaggagg aaaaacctgg catgtttcgt
     9841 cattactatt cctcaaaaaa ccaaaagcgc gttatttacc caaaaaggca acgcgttaac
     9901 tcgcagaaag aaaaatacag ttcgctatcc tacaaattat cattcgtcga tgtaaggaat
     9961 agttatgaat acaggcgtct caaggcacat aaacacaaaa aaagattaat attctactgt
    10021 tttattttga cgcgggttga aagaggcaga attaaaaaat cgtaaattga agtata
//
LOCUS       DAZKFZ010000171        10075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000171 DAZKFZ010000000
VERSION     DAZKFZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            177..392
                     /gene="bdm"
                     /locus_tag="ACHOQW_003669"
     CDS             177..392
                     /gene="bdm"
                     /locus_tag="ACHOQW_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP9574672.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            494..631
                     /gene="sra"
                     /locus_tag="ACHOQW_003670"
     CDS             494..631
                     /gene="sra"
                     /locus_tag="ACHOQW_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP9574673.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            788..2485
                     /gene="maeA"
                     /locus_tag="ACHOQW_003671"
     CDS             788..2485
                     /gene="maeA"
                     /locus_tag="ACHOQW_003671"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9574674.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2619..3629
                     /gene="adhP"
                     /locus_tag="ACHOQW_003672"
     CDS             2619..3629
                     /gene="adhP"
                     /locus_tag="ACHOQW_003672"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP9574675.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3775..4059
                     /locus_tag="ACHOQW_003673"
     CDS             3775..4059
                     /locus_tag="ACHOQW_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9574676.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(4287..4940)
                     /gene="fdnI"
                     /locus_tag="ACHOQW_003674"
     CDS             complement(4287..4940)
                     /gene="fdnI"
                     /locus_tag="ACHOQW_003674"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP9574677.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4933..5817)
                     /gene="fdnH"
                     /locus_tag="ACHOQW_003675"
     CDS             complement(4933..5817)
                     /gene="fdnH"
                     /locus_tag="ACHOQW_003675"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP9574678.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5830..8877)
                     /gene="fdnG"
                     /locus_tag="ACHOQW_003676"
     CDS             complement(5830..8877)
                     /gene="fdnG"
                     /locus_tag="ACHOQW_003676"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8290..8292),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9574679.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9109..9990
                     /gene="yddG"
                     /locus_tag="ACHOQW_003677"
     CDS             9109..9990
                     /gene="yddG"
                     /locus_tag="ACHOQW_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP9574680.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2457 a         2647 c         2537 g         2434 t
ORIGIN      
        1 tattaatcaa ctcatcacaa taataatacc tatcaatatc atggggttaa aatataaaaa
       61 tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc tgaatgtgct
      121 ttataaatct gcgatccgta gcagacacta taaatacaca gacacggaga atcactatgt
      181 ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat gccattgagc
      241 aaggccttcg ggcagagcac ggtgttgtca ctgaagatga cattctcatg gagctgacca
      301 aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa acgatcaatt
      361 atgtggtcag tggccagcac cctacgcttt aagatgctat gcttgatcgg caacctaatt
      421 taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact agcaacagga
      481 ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac tggaccataa
      541 aatatctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg taaataaccc
      601 aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc aatgacgatt
      661 aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact tggggccgac
      721 gccctggcgg taaagcaaag acgataaaag ccccccaggg atggatattc aaaaaagagt
      781 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      841 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      901 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      961 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
     1021 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
     1081 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
     1141 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
     1201 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
     1261 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
     1321 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
     1381 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
     1441 cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
     1501 gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
     1561 ccgctatcgc aatgaaattt gttcctttaa cgatgacatt cagggcaccg cggcggtaac
     1621 agtcggcacc ctgatcgcag caagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1681 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1741 ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg tcgatcgctt
     1801 tggcctgctg accgacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1861 gcgcgaaaac ctcagtgaca gggataccga cagcgatgtg ctgtcactgc tggatgtggt
     1921 gcgcaatgta aaaccagata ttctgatcgg cgtctcagga cagaccgggc tgtttacgga
     1981 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtccaa
     2041 cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggactg aaggtaacgc
     2101 gctggtcgcc acgggcagtc cgtttaatcc agtggtatgg aaagataaaa tctaccctat
     2161 cgcccagtgt aacaacgcct ttattttccc gggcatcggg ctgggtgtta ttgcttccgg
     2221 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg cccagtattc
     2281 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctaaaagata ttcagaaagt
     2341 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtgaa
     2401 aacgtctgcc gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     2461 cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac cgggctattt
     2521 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     2581 cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca gttgttacga
     2641 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2701 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2761 ttggtgacaa aaccggcgta attctgggcc atgaagggat tggtgtggtg gcagaagtgg
     2821 gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttctacgaag
     2881 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2941 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     3001 cggtaaaagt gccagatggt ctggactcgg cggcggccag cagcattacc tgtgcggggg
     3061 tcaccaccta caaagccgtt aagctgtcaa aaattcgtcc cgggcagtgg attgctatct
     3121 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc gaagaatgtc tttaacgcga
     3181 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atgggtgcag
     3241 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     3301 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     3361 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     3421 atatcccacg tcttgtgctg gatggcattg aggtggtcgg ttcgctggtc ggcacgcgcc
     3481 aggatctaac tgaagccttc cagtttgccg ccgaaggtaa agtggtgccg aaagtcgccc
     3541 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     3601 gccgtatggt gattgatttc cgccgctaag aggcctttgc tgcgactccc atgttcgggt
     3661 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3721 aacaataatg atagtgttcc cagagaagga acgctactga gagtaaagac actaatgaaa
     3781 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3841 agcctgcgcg agtttgccag agcaatggaa attgctccct caacggcaag ccgattgctg
     3901 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3961 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc tgcaaaaacg
     4021 gtggatgtgt cgcgattacg ccgtctggtt gcgcaataaa aaatggcgcc ttttacagcg
     4081 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     4141 cgttcactgc aatctattgg atttgaacgg tttgtaggcc ggataaggcg ttcacgccgc
     4201 atccggcatg aacaaagcct acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     4261 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     4321 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     4381 ttcccttcga tcatcccttt aatcgatcct ttcacccaga atgccatata catatggatc
     4441 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     4501 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     4561 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     4621 ccgacatccg ccactttatg ctcattgcct ttcaatactt cgacaatgtt taacagccac
     4681 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4741 gcgacgaaaa tcgcaatgcc gaagaatggg tgcaaaatgc gtcccatctg cggcgtaccg
     4801 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4861 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4921 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4981 aataccgatg tagtggaaaa tcaaccctgc aaaagtggca ataaagccag ccgctgccag
     5041 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     5101 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     5161 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     5221 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     5281 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     5341 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     5401 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     5461 aatgattgca ccggcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     5521 gccatcttta cggatcagcc actccagctt gccgctctgt tcggtttcgc taaagcgcat
     5581 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     5641 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5701 acaggtggaa acgtcgataa gttttgcgac ttctgctttg taatcacgca cctgagaagg
     5761 cggcgtgatg gagttagttg cggacctttt gataatgtcc tgcgtttcca tagccatctg
     5821 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5881 gtttgcatca ccgacattcg gcgtcagagt gttagctata taacctttac gcgcgacacc
     5941 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccat ttacattcag
     6001 cggtttcaga cgacgcgtta ccacagccac cgcgcggata aagccacgct tgcttgagac
     6061 agtgacacga tcgccattat taatgccttt cgccgccgcc agcgtttcgc tgatttccac
     6121 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     6181 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     6241 cgcgtcttgt tcatacagac gaaccaccgg gttagaaacc acgttcggat gcagcgggtt
     6301 agttcccagc ggcgtttcaa ttggctcgta gtgttccggg aacggacctt ccgccatttt
     6361 gttgatggca aacaggcgtc ccatcccttc cggctgcatg ataaacggcc cggttggcgt
     6421 acccggtgcg gcattgccga agtcagggat atcgttaccc gtccacttgc tgccgttcca
     6481 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatctgccg aagcacggtt
     6541 gtagagtacg cgacggttaa gcggccacgc ccaggcccat cccagcgtat tccccagacc
     6601 ggacgggtcg gagttatcgc ggttagccat ctggttgccc tgctctgtcc agctaccggt
     6661 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6721 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccagcgcata
     6781 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6841 gctcatcttc atcagcggtt ctacgccttt accaccttca gcctggtaca gctcgcgcag
     6901 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6961 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cttcttctgc
     7021 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     7081 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     7141 catgtacttc agcttgctca ggcagctcac cactttgttt ttgtccggga aggacgcaac
     7201 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     7261 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     7321 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     7381 gctccagtag ttcacctgac cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     7441 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     7501 cagacctaag tcagtcagac cctgaatgtt ggagtgacca cgcaatgcat tcacgccgcc
     7561 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     7621 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7681 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7741 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc tctttcagca ggttccacac
     7801 acagcgcgga tgagtcagtg tttcatcgcg ttttgcatag ccgttttcat cgaactgata
     7861 gttccaggac gacttatcgt actggcgttt ttcagcgtcg tagccgctga acagaccgtc
     7921 ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgct taacgtattc
     7981 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     8041 cgtaccggaa cgaataggcg cgtagatatc cgccacagaa gcggtacgcg taaaacgggg
     8101 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     8161 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     8221 atccacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     8281 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     8341 tttctgggtc agcatcccgg tttcgttgct ggcaccggaa gcacacagca taccggtaga
     8401 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     8461 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     8521 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcgctgt tgacgtaatc
     8581 cagcaaaccg gcccctttcg ggcacagcgc cccacggctt accggatgat ccgggtcacc
     8641 ttcaatgtga taaatcgctt ctctggcgtt tttcgcgcca tcacccaggc tatacatcaa
     8701 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8761 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctactgt
     8821 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8881 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8941 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     9001 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     9061 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     9121 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     9181 cgcggtgtca gtgaggggct cggcccggtc ggtggcgcag ctgctatcta ttcattaagc
     9241 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcaaatccc gaaaggctat
     9301 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     9361 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     9421 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     9481 ggattattat tagccctcgt cggcgtctgc tgggtgttag gcggtgacaa tgggttacat
     9541 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgttcatt
     9601 ggtgcgttta tctgggcagc ctactgcaca gtaacgaata aatacgcacg cggatttaac
     9661 ggaattaccg tttttgtcct gctaacggga gcaagtctgt gggtttacta ttttctcacg
     9721 ccacaaccag aaatggtatt tagcacgcca gtcatgatta aactcatctc tgcggcattt
     9781 accttaggat ttgcttatgc tgcatggaat gtcggtatac tgcatggcaa cgtcaccata
     9841 atggcggtag gttcgtattt tacgcccgta ctttcctcag cgcttgcagc cgtgctgctc
     9901 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctga tggtctgcgg cggttccctg
     9961 ctctgctggc tggcgacacg tcgtggctaa aataattgcc gggtctacgg cccggcaatt
    10021 aatcagataa accccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcac
//
LOCUS       DAZKFZ010000172        10042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000172 DAZKFZ010000000
VERSION     DAZKFZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.172"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            101..1150
                     /gene="allD"
                     /locus_tag="ACHOQW_003678"
     CDS             101..1150
                     /gene="allD"
                     /locus_tag="ACHOQW_003678"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP9574681.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1172..2407
                     /gene="allC"
                     /locus_tag="ACHOQW_003679"
     CDS             1172..2407
                     /gene="allC"
                     /locus_tag="ACHOQW_003679"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP9574682.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2418..3203
                     /gene="allE"
                     /locus_tag="ACHOQW_003680"
     CDS             2418..3203
                     /gene="allE"
                     /locus_tag="ACHOQW_003680"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP9574683.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3431..4576)
                     /gene="glxK"
                     /locus_tag="ACHOQW_003681"
     CDS             complement(3431..4576)
                     /gene="glxK"
                     /locus_tag="ACHOQW_003681"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP9574684.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4598..5723)
                     /locus_tag="ACHOQW_003682"
                     /pseudo
     CDS             complement(4598..5723)
                     /locus_tag="ACHOQW_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(5780..7141)
                     /gene="allB"
                     /locus_tag="ACHOQW_003683"
                     /pseudo
     CDS             complement(5780..7141)
                     /gene="allB"
                     /locus_tag="ACHOQW_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(7178..8569)
                     /locus_tag="ACHOQW_003684"
     CDS             complement(7178..8569)
                     /locus_tag="ACHOQW_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP9574685.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(8738..9616)
                     /gene="glxR"
                     /locus_tag="ACHOQW_003685"
     CDS             complement(8738..9616)
                     /gene="glxR"
                     /locus_tag="ACHOQW_003685"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9574686.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9717..>10042)
                     /locus_tag="ACHOQW_003686"
     CDS             complement(9717..>10042)
                     /locus_tag="ACHOQW_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9574687.1"
                     /translation="GFHLTGTRQALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQ
                     WADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRW
                     MDPYR"
BASE COUNT         2657 a         2654 c         2377 g         2354 t
ORIGIN      
        1 ctaactatat ttttttgcct gtctggatca cataatccag atattttccc tgtatgttaa
       61 ttgcagtcat gcttcacacc gtcgttaaaa aggaagacag atgaaaatca gtcgggaaac
      121 actccaccag ctaattgaga ataaactctg ccaggctggg ttaaaacgtg agcacgctgc
      181 aaccgtggct gaagtattgg tttatgccga tgccagaggg atccactctc atggcgcggt
      241 gcgcgtggaa tactacgcgg aacgcatttc aaaaggcggc accaaccgcg aaccggagtt
      301 tcgtcttgag gaaaccgggc cgtgctcggc aattttacat gccgacaatg ccgccggaca
      361 ggtcgcggcg aaaatgggta tggaacatgc catcaaaacc gcccagcaaa atggcgttgc
      421 ggtggtcggt atcagccgga tgggtcacag cggcgcaatc tcttattttg tgcagcaggc
      481 agcccgcgcc ggattaattg gcatttcgat gtgccagtcc gatccgatgg tggtgccgtt
      541 tggcggcgcg gaaatttact acggtactaa cccactggcc tttgccgcgc cgggagaagg
      601 cgacgagatc cttacctttg atatggcgac taccgtacag gcatggggaa aagtcctcga
      661 cgcccgctcg cgtaatatgt ctatcccgga tacctgggcg gtcgataaaa acggtgcacc
      721 aacaaccgat ccgttcgcgg tacatgctct gctccccgca gccgggccga aagggtatgg
      781 cctgatgatg atgattgacg tcctctcagg cgtcttactc ggcttaccgt tcgggcgaca
      841 ggttagttcg atgtatgacg atttacacgc cgggcgtaat ttggggcaat tacatgtagt
      901 tattaacccg aactttttct cctccagcga attattccgt caacatctta gccagaccat
      961 gcgcgaatta aatgccatta cccccgcgcc cggttttaat caggtttatt atcccggaca
     1021 ggatcaggat attaaacaac gccaagccgc cgtcgaaggc atcgaaattg ttgatgatat
     1081 ttaccagtat ttaatttccg acgcgcttta taacacgtca tacgaaacga aaaatccctt
     1141 tgcgcaataa ttattgagac aggactttct tatgattaca cattttcgtc aaaccataga
     1201 agaagcgctg ccctggcttt cctcttttgg cgctgaccca gcgggtggga tgacccgttt
     1261 actttattcg ccggaatggc tggaaaccca gcagcaatta aaaaaaagaa tggccgcaag
     1321 tgggctggaa acacgtttcg atgaagtggg gaatttatac ggtcgcctga gtggcaccga
     1381 atatccacag gaagtgattc tgagcggttc gcatatcgat accgtggtta acggcggtaa
     1441 cctcgacggg caattcggcg cgctggcggc gcggctggca attgactggc tgaaaactca
     1501 atacggcgca ccgctgcgta cggttgaagt ggtggcgatg gcggaagaag aaggtagccg
     1561 cttcccgtat gttttctggg gcagtaaaaa tatctttggg ctggcgaatc ctgacgacgt
     1621 gcggaatatc tgtgatgcca aaggaaatag ttttgtcgat gcgatgaagg cttgcggatt
     1681 tactctgccg gacgccccgc taactccgcg tcaggatatt aaagcctttg tcgaactgca
     1741 tatcgaacag ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg tgaatgcaat
     1801 tgtcgggcaa cgtcgctata cggtgacgct gaacggcgaa tcaaaccatg caggcaccac
     1861 gccgatgggt tatcgtcgtg atacggttta cgctttcagt cgcatttgcc atcagtcgat
     1921 cgaaaaagcg aaaaagatgg gcgatccgct ggttctgacc tttgggaaag tagagccgcg
     1981 cccgaatacg gtgaatgtgg tgccgggtaa aaccacgttc accattgatt gtcgtcatac
     2041 cgacgccgcc gtgctgcgtg atttcaccca acagttagaa aacgacatgc gggcgatttg
     2101 cgatgaaatg gacattcgta ttgatatcga tttatggatg gacgaagaac ccgtgccgat
     2161 gaataaggac ctggtcgcca ccctgacaga attgtgtgaa agtgaaaaac tgaattaccg
     2221 ggtgatgcac agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg taccaacctg
     2281 catgattttt atccccagca tcaacgggat cagccataac ccggcggaac gcaccaatat
     2341 taccgacctt gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac ttgcctggca
     2401 gaaataagga gtcataaatg ggatatttaa ataacgtcac cggttaccgc gatgatttac
     2461 tggctaaccg tgcgattgtg aaacacggta atttcgcact gttaacacca gacggtctgg
     2521 taaaaaatat tattccgggc tttgaaaatt gtgacgcgac aatcctctcc acgccaaagc
     2581 tgggtgcctc ttttgttgat tatctggtca cactgcatca aaacggcggc aaccaacagg
     2641 gcttcggtgg cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat atcactgcca
     2701 aagccgaagg caaaacattt tccttaagcg aaggtggcta tctttattgc ccgcctggct
     2761 ccttaatgac gtttgttaac gcccaggccg aagacagcca aatcttttta tataagcgcc
     2821 gctatgttcc ggtagaaggc tatgcaccgt ggctggtttc tggcaatgcc agcgaactgg
     2881 aacgcattca ctatgaaggt atggatgatg tgatcctgct ggatttcctg cccaaagagt
     2941 taggttttga tatgaacatg catatccttt cttttgcacc aggtgccagc cacggttata
     3001 tcgaaacaca tgttcaggaa cacggtgcct atattctttc cggtcagggg gtttataacc
     3061 tcgacaataa ctggatcccg gtgaaaaaag gcgattacat ctttatgggc gcttattctt
     3121 tacaggctgg ttatggcgta gggcgtggtg aagcgttcag ctatatttac tcgaaagatt
     3181 gtaaccgcga cgtagagatt taagtcatac cactcgcctg attattttta gctatgttgg
     3241 attttgccac ggtttatacc gtggcttttt ttgcaacata gcctgatgcg cttcgcttat
     3301 caggcctaca tgatctctgc aatatataga atttgcgcga ttttcaggaa ggatatggta
     3361 ctcatgccgc atccggcatg agtactgcac tcttatgcga tataaatcgc atccgcttta
     3421 aaggtaaggg tcagttttta attccctgac ctattttaat ggcgcaggca atatttcgcg
     3481 cgctgttgaa gagattggtt tcaccgctgg cgagcacttc ggctaaaggt gccagacgtg
     3541 gcaaaatgct gaataccgca tcaatgccgt actggtgcac cacttccacg ccatcaccca
     3601 atacgccagc aatcccaatc accggtacat taaactgctt cgccaccgac gcaacgccca
     3661 gcggcgcttt accgcctgcc gtttgcgagt caatgcgccc ttccccggtg atcaccagtg
     3721 ctgccccctg caccgcctgc tccaggttga ccgcccgcag tacaatctca atacccggtt
     3781 taatatccgc attgaggaat accgccgccg caatccccat accgcccgca gcgccaccgc
     3841 ccgccatctg gcagacatta atttcagttt gctgttgtaa aacacgggcg taattttgca
     3901 gcccctgttc aagttcttcg accatctccg gcgttgcccc tttttgcggg ccaaacaccg
     3961 ccgccgcgcc gcgtgcccct accagcgggt tatcaacgtc acatgctact tcaatatggc
     4021 agttcgccag acgcggatcg cattcctcca tctcaatgct tgccacgcgc gctaaattgc
     4081 cgccgttagc caccagcacc tgaccgtcgg catcaaggaa acgcacaccg agcgcctgcg
     4141 ccatgcccat accgccgtcg accgtcgcac tgccgccaat gccgagaata atatggcgaa
     4201 tgccgttatc cagcgcatga cgaattaact cccccgtacc aaaactggag gccagcaacg
     4261 gattacgctt ttcaggcgcg accagcatca ggccacttgc tgccgccatc tcaatcaccg
     4321 ccgttttccc gtcgccggta atgccataaa aagcattcac tttttcgccc atcggcccgc
     4381 agacttcaag cgtcacgatg ttgccgcccg tcgcggcgac catcgcatcc accgtccctt
     4441 cgccgccatc cgctatcggc aaacagatat agttcgcatc gggaaagagg gtcgaaaacc
     4501 cggctttaat tgcctgacaa catttttctg cactcaagct ctctttaaaa gagtctggcg
     4561 caatgacaat cttcattatc aatccttttt caacaaatta ttcgatgcgt tcccacggaa
     4621 taaggttatc cataagaaca gccagtaaaa tcccgaccaa taagccgtta ctgagcagag
     4681 gacgaagcgt taatggaagg tcttgcagat acacaggcgg taatgccatt aaaaatatgc
     4741 cgacaaataa cgctgccata aataaacgaa cgccgcgtgt aatcgccagt ctgggttaat
     4801 aaaccaatgg atgaaacaaa cggtgaaaat ggaattactg ccagcggcac ggttatcagt
     4861 gtcataaatc cggtcgccac aaagctacga cgataacgcg tattccccgc gccctgctgc
     4921 ggataaaaaa catccgtgcc ccgaatcgca ccgtaggtat tgctgatatt caccagacct
     4981 gtaatcaccg ccgtcagaat aatccccggc gacaaagcac cgccactgcc gagcgggaac
     5041 cactgccagt gcaactcacc ggagagcgaa tgcgaagaag gaaagcaaaa gtaccacaac
     5101 aaccagccgg ttatggtgcc gaccagcagg ccataacggg caaaacgttg cggcagaaag
     5161 ataatcatcg ccagtaccag gcacatcacc gccaccgaga gcgcgaacgg cggtaactga
     5221 attttaaaat tcgggtcggc tatgccaaac ggcagcccga gcataccttt gaaaaagata
     5281 gtggtcagct gcgcgcccag catcaacata aacaagacca tcaccgacgg cgtaaataac
     5341 cgcgccaggc gatgacctaa tccgctaaaa ccaatcaaca tcgtcagcac gccggagagt
     5401 gcaatcccca ccgccaggct ggtagcgata tcgttgatcg gtgtcccgcg cgatgcttcg
     5461 ccaagggtga tagttaggat ggttccccac cacaggcccc ccggcccttc cataatagcg
     5521 cgacgatgac cgcaaaacgc ctgagcgaag caggccagtg cggtagcaag aaaagcatat
     5581 tgcgtgagcg taagcaggct actttgcggc aactgaaaag cagaaagtag cgtaggagga
     5641 accacaaccg tgttgcaaaa aataaagaaa aaccactgaa atcctgataa caggctttcg
     5701 cggctaactg caaaattgaa cataaacaca ccttaaccgg agaatgcccg ccgcaaggac
     5761 gggcattgca ggggccagat tactgctgat gtttaaggat aaattgacct ttcggcgcaa
     5821 cagggaagcc ctgttcgatg tcgtaaatca catcaccacg taagatggtt ttcgtgatac
     5881 gcgcgccaat ggtacggcca acatacgggc tgactttgtg gcgatattcc agatcgtcat
     5941 tggtaagaac atagctgcta ttcggctgaa tgaagacgaa gtcggcatct tttcctgggg
     6001 cgatacggcc tttttgctgc agaccaaaaa tatctgctgc gttagtcgcc attaatttgc
     6061 cgaacattgg cagagacatt ccgcgtttct gtaccgcttc atcgaacatc acgtccatac
     6121 agttttgcag accggcaata ccgccccatg cttccatgat gttgccggct ttcatttccg
     6181 gagggcatgg tgagtggtcg gaaaccaggc ggtcgatttc accgttaaac agtttttccc
     6241 acatgccttt ctagttttcc agatcgcgga tcggcggtga acacttcgcc agagtaccga
     6301 tttcttcgaa ctgatcggta tccagtacga agtaatgcgg gcaggattca caagtaacgt
     6361 cctgaccttc ctgacgtgca cgagtcactt cttcaacgcc ttccgggctg ctgatatggc
     6421 aaacgtgcag acggcaaccg gcaactttcg ccagatacag cacgcggcga atcgcttcca
     6481 cttcggtaaa taccggacgc gaagccacat agtcatgggc agttacgcga ccttcacgtt
     6541 tcgcttcttc gcccagtgcg tcacagatca gcgcgttttc gcagtgcacc agcaccggct
     6601 gccccagttc gcccagcttc tgcgcacctt tgaagaactg ccagtcgttt acatcacgga
     6661 agtcgttgtc gataccgcga tcgccacagg tcgcaacgaa gcatttgaag ccgacaacgc
     6721 ccacttcatc cagctcatgc agacggtcga tgttgtaaga caccaggcca ccgagttgtg
     6781 ccgcatcgat agtcagcttg cctttagcgg catcgaactt cagttcaatt gaagcgcggt
     6841 caaccgttgc aggcagctgg ttgagcggca tttcgatcat ggtggtgata ccaccttttg
     6901 ccgctgcgcg agtaccggtt tcataacctt cccagtggct acgacccggt tcagaaatat
     6961 gggtgtgcgc atcaaccatg cccggcgaaa ccaccagacc agacgcatcc ataacttctt
     7021 ttgcatcgcc cagatcctga ccgatagcag caattttgcc gcctttaacg gcgatatcta
     7081 caacgcgagc ttcgttttct aaaataacgg tgccgttttt aatgattaaa tcaaaagaca
     7141 taacaaactc cttgtaaaat acagattggt ttttaactca agcaaaggcg acgatgacgc
     7201 cgacaaacca tgaaacacgc gataacggtt ccattaaggg aataaactta ccgccaagag
     7261 ataaaataac ggccaccaga gttactgaaa acgcggtgag gttaaaaccg ttatcgtaat
     7321 atttataatc gccaggtgcg gtgtacagtt catcaagatt aatttgccca cgcatcacca
     7381 caaaataatg cgccatcatg acaccaatta ccggaccaag cattccgccg ataatatcga
     7441 ggaacagata aatactgtcc tgattttcca ttaatttcca cgggcagatc agcaggctga
     7501 taatactggc aatcagtacg ccgtttttat aggtcagttt tgtcggtgca atggcagcaa
     7561 tctgataacc agccggaata atattaccag tggcgttagt ggagatagtc gtcatcagaa
     7621 taaccagtac cgcaaagaac gaggcaaaca ggctgtccca acgctgaaca atatccagca
     7681 cgttccaggt atcagcgccg taatgaatac tggctccggc aataatacat acccccgcga
     7741 ccgcaaacag aatataggcc acaactaaac ccagcgtttg ccccagcgct tgctcacgaa
     7801 acgagtgggc gttttgcgta aagtcggatg cgctcaccgc cggtgccgcc cagaccgcaa
     7861 ctaccgcgtt aatcaccacc aggaacaaga atccactgtt ttctgctttc tgaataccgc
     7921 tcggaatgta gtcaaagatt ggaccgagcc cgaccagcga aatcgcccaa atcgccatac
     7981 cgccgaaaac gatatagatg cacgggttaa gaatggcagt gaatttattt aaaactttgc
     8041 caccgccaaa acctatacca acgttgacca gccagaagag taagaaagta attaagcccg
     8101 gtagagaaag gcctaacaga gtgaaatcac caccgagagt caaaaatccc ggccagattt
     8161 tgccaatcag aatcaagcag gccagtgacc ccgcgtaaca ttgcaggcca aaccacataa
     8221 tggcagcaat accgcctctt aataatccgg gaaacagtgc gccacgtacg ccgtaagaag
     8281 cacgcaggat catggcaaac ggtacgccgt atttactgcc cgcagcgccg tttaatacca
     8341 ttaccgcggc aatgaaaaag gcgctgagga taattgccag cataatacta aaggtagaca
     8401 agccgagaat aaaaaagccg ccgaccatca cataattggg aacgttatga accgaaccca
     8461 tccataaggt aaaatagtta aatgttttcc aggtccgctg gctttgcgtt ttcggcaata
     8521 gatcttcgct atagccgcgt tgctggaata gttttctctg atgttccata aatccctctt
     8581 aatttcacgt atcgaataac gttgttttaa tctagctatg aggaaaaaat aaaataaaaa
     8641 tattattttt aatactgaag caacatcttt ggctgctttc ttttttaata ttgctggccg
     8701 attcattttt tgatatcggc catttttatt gcgggtatca ggccagttta tggttagcca
     8761 ttaattccag cgcctgcact aacgcagagt gatccaactg gctgccaccg tttgccgcgc
     8821 aggtattaaa taactcctgg caggtcgcag tgtttggcag gttcagagcc agtgccttcg
     8881 cactttgcag tgccaggttg agatctttct ggtgcagcgc aattttgaaa cctggattaa
     8941 aggtgcgttt aatcatgcgc tcaccgtgaa cttccagaat acgtgaggaa gcaaagccgc
     9001 ccatcagcgc ctggcgcaca cgtaccgggt ctgcaccggc ttttgaagca aacagcagag
     9061 cttcagagac cgcttcaata ttgagcgcta cgataatctg atttgccact ttgcaggttt
     9121 gaccgtcgcc gttaccgccc acaagggtga tatttttacc gagcagttca aacagcggtt
     9181 taacacgttc aaataccgct tcgtcaccgc caaccataat tgacaacgtc ccttcacgcg
     9241 cgccgatttc gccgccggag actggcgcat cgagataatc accacccagt tcattcacct
     9301 gacgagcgaa acgtttagtt tcaatcggtg aaatggagct catatcaaca atggttttgc
     9361 ccttcagcga ggctttggta caaccatttt caccaaacag aacttcttca acctgaggtg
     9421 tgtccggcac cataataaaa atgatgtccg ctgcttccgt tacctggcgc gcggtgtcga
     9481 cattaacagc accgagtgac agtaattcat cagcaaccgg tccaatggtc gtgacatgta
     9541 attgatgacc ggcacgcgcc agattaatgg ccatcggagt acccataatg cctaagccaa
     9601 taaatcccag tttcataatt aacctctttt taaatgtcgc tattcctgaa ttcagacaac
     9661 acgatcccct gccaacttag cagcctaaaa aagcattgtc tgaatagcgt tacgttttaa
     9721 cggtacggat ccatccagcg taaaccggct tccgtggtgg tttggggttt atattcacac
     9781 ccaacccaac cgttgtagtc agaattttcg attaccttaa agagataatc ataattaatt
     9841 tctccggtcc ccggttcgcc gcgatgcgga ttatcggcaa tttgcaggtg accaatttta
     9901 tcagcccatt gcgtcatagt gttggttaat tcaccttcca tccgctgcat gtgataaatg
     9961 tcatactgaa ttttcaaatt gcagcaacca acatcatcaa tcaatttcag cgcctgccga
    10021 gttccggtga gatggaaacc ag
//
LOCUS       DAZKFZ010000173         9893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000173 DAZKFZ010000000
VERSION     DAZKFZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..169)
                     /locus_tag="ACHOQW_003687"
     CDS             complement(<1..169)
                     /locus_tag="ACHOQW_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574688.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKR"
     gene            247..1341
                     /gene="ddlA"
                     /locus_tag="ACHOQW_003688"
     CDS             247..1341
                     /gene="ddlA"
                     /locus_tag="ACHOQW_003688"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9574689.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1365..1577)
                     /gene="yaiZ"
                     /locus_tag="ACHOQW_003689"
     CDS             complement(1365..1577)
                     /gene="yaiZ"
                     /locus_tag="ACHOQW_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP9574690.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1837..2145
                     /gene="yaiY"
                     /locus_tag="ACHOQW_003690"
     CDS             1837..2145
                     /gene="yaiY"
                     /locus_tag="ACHOQW_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP9574691.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2204..3298)
                     /gene="yaiW"
                     /locus_tag="ACHOQW_003691"
     CDS             complement(2204..3298)
                     /gene="yaiW"
                     /locus_tag="ACHOQW_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP9574692.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3311..4531)
                     /gene="sbmA"
                     /locus_tag="ACHOQW_003692"
     CDS             complement(3311..4531)
                     /gene="sbmA"
                     /locus_tag="ACHOQW_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP9574693.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4883..6040
                     /gene="ampH"
                     /locus_tag="ACHOQW_003693"
     CDS             4883..6040
                     /gene="ampH"
                     /locus_tag="ACHOQW_003693"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP9574694.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6041..6664)
                     /gene="iprA"
                     /locus_tag="ACHOQW_003694"
     CDS             complement(6041..6664)
                     /gene="iprA"
                     /locus_tag="ACHOQW_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP9574695.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6752..9694)
                     /gene="ehaB"
                     /locus_tag="ACHOQW_003695"
     CDS             complement(6752..9694)
                     /gene="ehaB"
                     /locus_tag="ACHOQW_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP9574696.1"
                     /translation="MHSWKKKLIVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKYTW"
BASE COUNT         2554 a         2451 c         2340 g         2548 t
ORIGIN      
        1 ttctcttcgt taacttataa ttaagagaaa aaaactcacc gcatggccat ttaagccagg
       61 aataaattag cttcccgttc atcatttttg tgatcttgcg cacatctttg agataacgca
      121 ctttcttcac cagtacgttg cacgctttgt cagcagaaga ttgcaacaat ctttcagaag
      181 gccgcgaatc ccccgttttg tcgtggtaat gtatgcactt tgtgttagac gggataggtt
      241 tttaagatgg aaaaactgcg ggtaggaatc gtttttggtg gtaaatcagc ggaacatgaa
      301 gtgtctctgc aatcggcaaa aaacattgtc gatgccattg ataaaagtcg cttcgacgtt
      361 gtgctgctgg gcattgataa acaagggcaa tggcacgtca gcgatgccag caattatctg
      421 ctaaatgcag acgatcctgc ccatattgcg ttgcgccctt cggcgaccag cctcgcccag
      481 gtgccaggta aacatgagca tcagcttatc gacgcgcaaa acggtcagcc gttgccgacg
      541 gtggatgtca ttttcccgat tgtccacggt acgctgggcg aagatggttc cttgcaggga
      601 atgctgcggg tcgccaattt accgtttgta ggttctgatg ttctggcttc agcagcctgt
      661 atggataaag atgtcaccaa acgtctgcta cgcgatgccg ggctgaacat tgcgccattt
      721 attaccctga cgcgcgctaa tcgtcacaac atcagttttg ccgaagtgga gtctaaactg
      781 gggttaccac tgtttgtaaa accggctaat cagggctctt ctgttggtgt cagcaaagta
      841 accagtgaag aacaatacgc aattgccgtc gatctggcgt tcgagttcga tcataaagtg
      901 atcgttgagc aagggatcaa aggtcgtgag atcgaatgcg cagttctggg caacgacaat
      961 ccgcaagcca gcacctgtgg cgagatcgta ctcaccagcg atttctatgc ctacgacacc
     1021 aagtacattg acgaagatgg cgcgaaagtg gtagttccgg cagccattgc gccagaaatc
     1081 aacgataaga tccgggcgat tgccgttcag gcttatcaga cgttgggatg cgcaggcatg
     1141 gcgcgtgtag acgtgttttt aaccccagag aacgaagtgg tgatcaacga gatcaacaca
     1201 ctgcctggct tcaccaacat cagtatgtat ccgaagctgt ggcaagccag cggtctgggt
     1261 tacaccgatc tgatcacacg tttgattgag ctggcgctgg agcgtcacgc tgcggataac
     1321 gcattgaaaa ccacaatgta atattttaac gcccgataat attcttattg ggcgttttca
     1381 gtttcttcat cgcgacgacg aataatcaac cccgccagcc agaaactaat cacccacgtc
     1441 accagaccga cggcataggt ctgccaacct tttgcttcaa atcccagtaa ccccaccacg
     1501 ccattaagaa tgaatataag gccaatggcg aacgcatagt agtgccagtc acggcggatt
     1561 tttacaggca gattcatggc tgctccgtta acgaaaaaca acatccttgc atattctgca
     1621 acaaccgtct gtggtgtaga caacattttc gcaattgtta ataaatttaa cttaaagttg
     1681 ctattttgtg actcgcaacg aagattcacg cttcgggaat attcccaaca tcaatttacc
     1741 atgattctga tattgacaat ctaaatgacc tgtcagactt gccataagcc gctggtatca
     1801 caccagaaga gaagcagatg acttccggag gtttttatgg ctgatttcac cctgtcaaaa
     1861 tcgctgttta gcggaaaata tcgcaatgcc tcttcaacgc ctggcaacat tgcctatgcg
     1921 ttgtttgtgc tgttttgctt ttgggctggg gcgcaattgc tgaacctgtt agtgcatgcg
     1981 cccggcgtct atgagcgttt aatgcaggtc caggaaacag gtcgcccacg ggtggaaatt
     2041 ggtttaggtg tcggcaccat tttcgggctg atcccgtttt tagtaggctg cctcattttt
     2101 gcagtggtgg cgctatggct gcactggcga catcgccgcc agtaagattt acgttgccag
     2161 atacggccta aaacgcctta tctggcatga cattcctgtt tgttcactgt ttcatacaac
     2221 gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt agtcaggttg cgggtgattt
     2281 tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg gagtgattta cccgttttcg
     2341 cttcggcaag ttgataaact ttcttgtaca gcgccgtctc ctcaaaagag aagctgtcac
     2401 ctttctctag ctgacgacga atttcgctgt cgctcatccc cagttttgcc gccagtttgc
     2461 gcgttgccag ttcggttttc ccgggttctt tactgtcgta acgaattaaa tcaccgtcca
     2521 gtgccagctt cacgccgctg gccttactga ccgcgttctg aaacgcggca ttacggctgg
     2581 cgtaccagcc agcgttaaaa tcagcaaaac ggtatatcgg tgcgctataa ctggcgggat
     2641 aattcagtaa atggtaagta ccaaaccaca acccgccgcg gcgactaaag acttcctgac
     2701 ggactgtacc gtccattttc cacggatacc ctttggtatg ctgttcggca aaagcaatgc
     2761 tgacctgcat cggcccaccg gtgcgcaccg gattaagcga accaaacaac gtctgcccca
     2821 ttggcaccat gctgatcaga tcgtcaaaaa tcccgctcaa ttgcttttct gtacgcaccg
     2881 aatctaaccg ttcgctataa cttttgccgt ttggcgattt gatttttagc gcagtatgaa
     2941 ccagaaaagc aggaatgtgc atccgttcag cacgacggtc aatctcttgc caggcgatct
     3001 tgcttaagcc cggaacggcc ggatctgcct gataatttga ctcctgctgc gccaccgcca
     3061 gcaccgagca gacgttttcc agcgtcggtg ccagcccctg actttcaaag gtggtggcga
     3121 gatctttcgc ccaggcatcg cggtctttca cgctcgcagg catcttctgc cgcaccacgc
     3181 tagccacatc tatcgctttt tcgccctttt tcaacggctg tggcgcctga ctgctacacc
     3241 ccgctaaaac cagaaccgcc aaaaatgaaa gcgaagacag atgattgact cgcgacatta
     3301 cttctccttt ttagctcaag gtatgggtta cttcctgaat tttgtcacca tccagctcat
     3361 gttcaaagct gcgcagacgt ttgtagatag acatcaactc aaccagtgtg gtccatgagt
     3421 taatcaggta ctggaaagca ccgcgaacct gaccaaaaac gttggtaatc tgcgtcatca
     3481 ggccgagcgt aatcgtaccg gcaacaattg acggaaacag caagaacaaa ccgaaaacgt
     3541 tatcgacctg caaatagaga atgcgagcga tgttgaaata catatagtga aaatagaggc
     3601 ggaaatagtt tttccgtacg gcgctaaaca gctcgcgtac cgttggcggc gtcgcgcgcg
     3661 tggcatcgtc ttcaccataa accagctctt tacggtaggc agcctctaca cgctggtttt
     3721 taaactccag ccccggcagt ttgatcccta ccactgccag caatccggtc cccatcagcg
     3781 accagacgat agcggcaatc accagaccat acggaatgtg cccgataatc ggcagctccg
     3841 gcacatgcgc ggagagcgtt accaacaccg gcaggaaggc gatcaacgtc atgatggcgt
     3901 tgataaaact gacacccata ttctccagcg ttgaagcaaa acgcatggtg tcttcctgca
     3961 cacgctgtgc ggcgccttcg atatgacgca gttgttgcca gttcgccatg taatattcgt
     4021 tcatcgccgt acgccagcgg aacacgtagt gactgacaaa gaagttattc agcacactga
     4081 tcactaccgc gatcagcgca atccccagaa agacgcccac ttcgcggtaa aattgttcga
     4141 tagtgacttt atgcggcgaa cttagcgcgg tttgaatcag atcatagaac ggtgcatacc
     4201 aggcgttgac ggcaacccca acttccacca aaaaccaggt gacgaagatg atcagtgcag
     4261 taccgagtat tgaccagtat tgccaacggt gcgggctgta gataaaccag aacaatgcaa
     4321 aaagtcctac gcaaacaatg tagtaagcgt aaaaaatcag gaaatccaac gaccagaaac
     4381 gcgcggcgct aatcgggatc tgcccggaag cgccggtgat acgcgccacc cagtcccccc
     4441 caccggcttg ccagaagata acggcaatca atgcccaaac aaaggccgag agaaaaaacg
     4501 ttcccggctt tgggaaaaaa gacttaaaca tatgcactcc tgctaacttc ttatcgtttt
     4561 aacaactgct gtgtattaac cgcatgaccg ctacaaagcg tcagccaaat gaaaccgccc
     4621 attgtgactc atagcaaggc gcttagttcg ccagcctggc cataagaatt gtttcgacgg
     4681 atttcacaaa ccaaagattg tttacataag cggattaaca cgcgcaggat cacaaatgca
     4741 ggcattggtg agcaggaaaa agatcgtgtt tacgcgccgt tacgttttta acactattat
     4801 tgcactaatt cgccctttgc aatccatcaa tgagtagtat aaatacgctc agttaccttc
     4861 attcaattta tggacaccac cgttgaaacg tagtctgctt ttttctgccg tgctgtgtgc
     4921 ggcgtcattg acctccgtcc atgcggcgca gccgatcact gaaccggagt ttgcctctga
     4981 tattgtcgat cgttatgccg atcatatttt ttacggcagc ggtgccacgg ggatggcgct
     5041 ggttgttatc gacggtaacc agcgcgtctt tcgcagttat ggcgaaacgc gacctggtaa
     5101 taacgttcgc ccgcagctgg attcggtcgt gcgtattgct tccctcacca agctaatgac
     5161 cagtgagatg ctggtgaaat tgctcgacca ggggaccgtg aagttaaacg atccgctaag
     5221 taaatatgct ccgccaggcg cacgcgtgcc aacctacaat gggacgccca ttacgctggt
     5281 caatctggca acccatacca gcgccctgcc ccgtgaacag cccggtggcg cggcacatcg
     5341 tccggtgttt gtctggccaa cgcgcgagca acgctggaaa tacctttcta cggcgaagct
     5401 gaaagccgcg ccaggcagtc aggcggcgta ctctaacctt gcgttcgacc tgctggcaga
     5461 tgcactggcg aatgcgtccg gcaagcctta tacccagttg tttgaagagc agattacccg
     5521 tccattgggg atgaaagaca ccacctacac tccctcaccg gatcagtgcc gccgtttaat
     5581 ggttgctgag cgcggtgcca gtccgtgcaa taacacgctg gcggcaattg gcagcggcgg
     5641 cgtctattcc acgcctggcg atatgatgcg ctggatgcag cagtatttgt catctgattt
     5701 ttatcagcgc agtaaccagg cagatcgcat gcagacgttg atttatcagc gcgcgcagtt
     5761 tacgaaagtg attggcatgg atgtccccgg caaagcggat gcgcttggtt taggctgggt
     5821 atatatggcc ccgaaagagg gtcgtccggg gattattcag aagacaggtg gtggtggcgg
     5881 tttcattacc tatatggcga tgatcccgca gaagaatatt ggcgcgtttg tggtggtcac
     5941 tcgttcgccg ttgacgcgct ttaaaaatat gagcgatggc atcaatgatc tggtgaccga
     6001 gctaagcggg aataaaccgt tggttatccc cgcatcctga ttaatactct gaaggcaaac
     6061 ggttgatagc gaccagtttg cctttattca tttcgatata gccgccttta cgcaaagcag
     6121 cgagaacttc tgcgacgaca gaacgcgata tgcgtgtacg ttgatggata taattcatca
     6181 caccaatacg tgatcgcaat tcttcattcc agtcaatcat tgataataat gttgcgcgga
     6241 tttgttcgta ggaattatgc ccaatgagct gcacgtcgcg taattccaga attctatttt
     6301 gccaggctaa ccagtaaaaa gcgtctcgcc agagttgatt ttgttcaata agcgtaatgg
     6361 tttgtttggc tggtagatga tatcccgtac aatttccttc tgatattaat ttgtatggta
     6421 tatcgttttt cattaaacca tcagccagcc ccataatata aggagcctgg gtaataccga
     6481 taagtacgtt ttcttctcta cgcagagaga taactccctc taaaataacg aaagtatctt
     6541 cgttattgac atcactggaa aatataactt gcttttcatt attaaactcg aagcgcgtac
     6601 cgtatctgga caaacattta tcgagcttac caaattcctg aagaggttta actacagata
     6661 acatttgcgc gtcctttgca gtaatgcccg tcaaatcctt gacgggcatt atttagatta
     6721 aattaccagt atttcttcgg agtgaagaat attaccaggt atatttaaca cccacgttcg
     6781 cggaccagtc ttgatctacg tcaccaccac cgaggtagtt agcatcggta taggcgctga
     6841 agttcttggt gaagctgaac tgagtaccca gaccaacacg taccgcagac ccttcagtac
     6901 cgttatcgat ggaatcaccg ttcacatcgt tatcgttgtt agagtcgtcg tagacgtaag
     6961 ccagtttgaa gtacggagtc agagcctgat cttcgctgta ggtgaaggta taacctgcat
     7021 ctacacccag ttcataacgc atgctgtcgt aagactgacc gtcaactttc atgtcgttgc
     7081 tcagctggta gtcatcacca gactggaaca gaccagaaat gctgccgtaa ggagtcacgt
     7141 aaccagcatc acccagtttg aagtcgtaac cggctttcaa accgaagccc caagcgtcgg
     7201 agttggtgct accgtcaacg taagtaccgt tgctcatggt tgcagacagg tcgttgttga
     7261 agtgagagta gctcaagcta ccatcaacaa agacgttgtt cgcgaagtga gcagaagagt
     7321 agatgtaggc agtctggctg tcttgatcca cctgaccaga acggtcattc atgtcacctt
     7381 tagcgaagcc tgcagccgca ccgacgatcc acttagcgtt gttaccgtca attttggtat
     7441 caacaccgac catgatgccg ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt
     7501 tgaagttacc accgaagtag cttacccatg cgccgccgtt atcagccagg ccatgacgag
     7561 agttggtcaa acgagtacca acggtgtctt gttccaggtt ccagatattg gtgttcgcag
     7621 atgggatgct cagcgccatg ttagcgtagt cggtcaactc catctgttgc agaacaacgg
     7681 tgttaccgcg ctgttcagcc tgataggtgt atgcacccag gtcagctttg ttagcagcag
     7741 agaaggtcgc gttgctgttt ttgtcgttga cgtagatcag ctcgttgccg ttgtagtcag
     7801 cgatagaacc agcaccagta gcgttgtcga tacgaacttt gtagttaccg gtcgcggctg
     7861 caacatggtc accgttgtta tcaacttcgt tctgaccagc ttcgatagaa gcagtgttgt
     7921 cgttatcgcc gttaccgtta atagtcaggt gaccatcaga gttcattgcg attaagccat
     7981 aaccgtagtt tgacttggta gtatcgttgg tacgatcgtt gaccagatcg gtatccatta
     8041 cgtaatcact gctatggata tcgaatacac cagcgtgaat gttgccgtta gtatcagtgg
     8101 tactggtcag attcagggta tcggcctgga acgggttagc atcgaactga tcaacgttaa
     8161 tatccaacag accgtcgtta gttacggtga tggtatttgc gtacagtgca gcatcttcta
     8221 aaccccagcc cgcagaaaca tcatcagaca ttttaacggt actattgtcg atggtcaggg
     8281 tattagtcgc tacatgaccg tcttcaccga tgttcaggtt agaaccgcca gtcagagaga
     8341 cagtgtcaga gatcagacga gagtctgcaa cattaacttg ggaaccgctg ttaatactta
     8401 aagtatcaat cagagaagat tttgttgtat cccactctga accaccgttc aaagtcacat
     8461 taaacaaacc gctctggtaa acttcgtttc ctacgatatg gccattttcg tcatattgag
     8521 tattgatgtt atcaacaccg acagttgatg aaggccacag gctgttagct gcaatatcga
     8581 gcagagttgc agttgcttca gtaccaacag catatccgtc gtaagaacca tcaccatctt
     8641 catcaaccat atgaacagac attgcagcgc caacccactt gctgccgttg ttcagagtca
     8701 catccatacg gtctgtgcca tcccaaccgt tggtatctac atcaccatca gcatcgcgat
     8761 agctgttagc accttgcggg aagaagtttt catcgaaatt actggagaaa acaacatcac
     8821 ccatcagtgt tgagttgtct aaagttactg tagtctgcat cgcattatca gcatacggat
     8881 ttgcgattgc ggcaattgca acatcgtctg cagtcagcgt gttgctatag ttgctggcat
     8941 taccagtatg gccaaaccaa ccagtagtac cttcatcagt ccatgaacca gaggtcacag
     9001 tagaatcttt aacagtaata gtgttattaa acacttcgct gctgtttacg ccagtgctga
     9061 ctgtgttgtc gtcaatatct tcccactcat aaccctgagt cagcgtaata cctgctacat
     9121 gagagttatt agtgatggac agatcaactt cctgatccag tgtaatagca gtacccatat
     9181 cgtaaacatc tacaacatga gtgtcagcgg cattattata ggtaccgttg taagtataat
     9241 gctcgtagtt gtcatcgatc gttgaattat caacgctcag tgtcagacga tcataaacat
     9301 aaccagtagc acggtcatca gcacaatcag tagtcatgca ctcagaggta atcataccgt
     9361 gaatagtgct gtttttgatt gtcagactat tggcgttggt attggtagaa ataccgtcgt
     9421 ccagatagta ggtagaaatc acgccgttaa cggtagcgtt attaatgacc gggtagatat
     9481 caccgttgta atagctatca gcggcatagt tgttccagcc tacataacca tcatagtaaa
     9541 cgtcatcagc ataggtggcg tcgttgtagt gatggaaagt attgtaagtt tgaccagaaa
     9601 tatcgttggt cgctgcatta gcctgagagg tgatagccag agtgcaagcc aatgctaatt
     9661 gtgatactat aagtttcttt ttccaggagt gcatttgtca tccctcctca gggacgtaat
     9721 aagttgatcc atcaattatc aatgcataga aaacatctta tgtttcgcgt tgacgattat
     9781 gcggcgttat agaggaatgg ttcaattatt ctttccccta agtccgattg cggacatata
     9841 taaaaatatc tatacgcaca aagaataagt tgacaaaaac actgcatttc aac
//
LOCUS       DAZKFZ010000174         9829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000174 DAZKFZ010000000
VERSION     DAZKFZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(160..2121)
                     /gene="topB"
                     /locus_tag="ACHOQW_003696"
     CDS             complement(160..2121)
                     /gene="topB"
                     /locus_tag="ACHOQW_003696"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9574697.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2126..3169)
                     /gene="selD"
                     /locus_tag="ACHOQW_003697"
     CDS             complement(2126..3169)
                     /gene="selD"
                     /locus_tag="ACHOQW_003697"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP9574698.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3286..3837)
                     /gene="ydjA"
                     /locus_tag="ACHOQW_003698"
     CDS             complement(3286..3837)
                     /gene="ydjA"
                     /locus_tag="ACHOQW_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP9574699.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3998..5854
                     /gene="sppA"
                     /locus_tag="ACHOQW_003699"
     CDS             3998..5854
                     /gene="sppA"
                     /locus_tag="ACHOQW_003699"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9574700.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5970..6680
                     /locus_tag="ACHOQW_003700"
     CDS             5970..6680
                     /locus_tag="ACHOQW_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFP9574701.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            6812..7828
                     /gene="ansA"
                     /locus_tag="ACHOQW_003701"
     CDS             6812..7828
                     /gene="ansA"
                     /locus_tag="ACHOQW_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9574702.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7839..8480
                     /gene="pncA"
                     /locus_tag="ACHOQW_003702"
     CDS             7839..8480
                     /gene="pncA"
                     /locus_tag="ACHOQW_003702"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP9574703.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(8573..>9829)
                     /gene="ydjE"
                     /locus_tag="ACHOQW_003703"
     CDS             complement(8573..>9829)
                     /gene="ydjE"
                     /locus_tag="ACHOQW_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9574704.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT         2436 a         2614 c         2444 g         2335 t
ORIGIN      
        1 ccaggatggt tttgtaataa aacttttttc atcacattcc ctgttattac atactttaga
       61 ggcgataatt ttatccatgc aaaaaaaata tccaacgaat tttcttgatc cgctggatat
      121 ttcagaatat gactcgaata gcacgaaaga ttcgctcgct tacgctatcg ccccgcttcc
      181 ggcttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag cctttttctt
      241 atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca ccggagtacg
      301 tttggcttga tcaataagct gatatagcgt ccccaccagc ggctgcataa agtcctgata
      361 gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg tcatgtccgg
      421 tcgcgtagcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg tggagtggat
      481 atagcgccct tttttgatga ggaaaccacg cttgaacaac agttcaataa tcccggcacg
      541 cgttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat ctttgtcctg
      601 cacaaagcgc gcgatcccgg tcatcgccga aagcagtgtt gcatcggtaa aatggcgcgg
      661 cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat cgcctttcgc
      721 caccacaggc aatggcgtac cgtcgttttc ttcatcgcgc tctttgctgc ctaacagcgt
      781 gcgccagcct gcttcagcaa gaaaacgcgc tttagcgaca aatttgcctt tggcaatgtc
      841 cagttcgata acacacttgc ggaacaccgc atccgggcag aactgcatca gatactgacg
      901 ggaaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag aactccgtgc
      961 ggtcggaatg atggcgtggt gcgcatcgac ctttttgtca tcccaacagc ggttgcgtat
     1021 atctggatct accactggct gcggcaacag atctggtgca tgaacgctga tggcattcat
     1081 caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg aacgcggata
     1141 agtgattagc ttgtgcgttt cgtacagctt ctggcagata tcaagcacgt tctgcgcgct
     1201 cagaccaaag cgttttgccg cttcaatctg caacgccgaa agcgaaaacg gcagcggcgc
     1261 ggattctgat tcccgtttat cgttatagct ggtgacaata gccggttgac cactaatgcg
     1321 gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat cctggtacgg
     1381 ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag gtgtcacgat
     1441 atgtgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt catcgcggcg
     1501 caccaccagc ccaagcacgg gcgtctgcac gcgtcccacg gaaagcacgc cctgataacc
     1561 ggcattgcga ccgagaatgg tatacgcacg ggtcatattg atgccgtaca gccagtcggc
     1621 acgcgctcgc gccagcgcag aaacgcacag cggtacaaac tcactgttgg aacgaagacg
     1681 gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac gctgcacctg
     1741 ctggcgcttt tccggtgcca gttgtagata gtccagcact tcatccacca gcagttgccc
     1801 ttcgcgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcaggt accgtttgat
     1861 gacgttaagt tgtttggtca cggagggtcg gggctgtaat tgccactttt ccgggacaat
     1921 cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg gctgcgcctg
     1981 ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact cgataaagcc
     2041 atcgcctttc cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca gactcggttt
     2101 ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga ccgccgcgtg
     2161 ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct gtagctttga
     2221 cctcattttc ggcttccggc atgaccgcca gcagcaaacc gccagaagtt tgcggatcgc
     2281 acagcaaatc gcgcacttca cgcggcattt cacccatcag atgaccgtag ctggcaaagt
     2341 tacgttcggt gccgccaggc actgcaccca acttaatgta ctcttcaacc cccggcaatt
     2401 tcgggatcgc gtcatagtca acgcgcgcct gcacacctgc gccctgacac atttcgctca
     2461 aatggcccag caggccaaag cccgttacgt cggtcatcgc ttttacgcct tcaatgttgg
     2521 caaaggacgc gcctgcgatg ttcatccggc acatcacttc cgtcgccagt ccctgatgtt
     2581 ctggtttcag cagtgatttt ttctcagccg tggtaagaac gccgatcccc agcggtttcg
     2641 tcaggaacag tttacatccg gcttgtgcgg tactgttttt cttcacccgc tcggtcggta
     2701 cgatccccgt taccgccaga ccaaaaatcg gctccggcgc atcgatggag tgaccgccag
     2761 ccagcgcaat acccgcctga cggcatgcat agcgtccacc ttcggttact tcgcgggcaa
     2821 tttccgggga aagtttgtta atcggccagc cgaggatcgc aattgccata atcggtttgc
     2881 cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca aaatcgaaag
     2941 gattatcaac gatcggcata aagaagtcgg tggtactgat aacgctggtg ccattgccca
     3001 gatcgtacac cgccgcatcg tcgcgggttt cattacccac aagcaaattc ggatcaacaa
     3061 acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt ttacagccgc
     3121 aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg gacatctcct
     3181 gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag tgataagtga gagtgtctga
     3241 attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga aataagtgac
     3301 aaacggcgtc gggtccggga cgtttatcga cgtagatgct ttcagctgcg gcgtgccgag
     3361 gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatgcttcac gcactaccgg
     3421 actttcagtt aatgcgccac tgcgccagat gccgccaaac ccctgggcaa cggctgccat
     3481 ttgcatcgcc atgaccgcgc atccggcaga catttcctgt tcccatcgcg ggactttatg
     3541 attttcttcg catttcgcca ccaccgtgat gatgagcggt gcgcggaacg gcgcattacg
     3601 ggctttgtcg atagctttgt catcactacc ggcagcaatc gccccctgtt ccagtacggc
     3661 gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa tgccacggtt gcatggactt
     3721 atggtccggc gcgcgcatac ccgcacgcag gatgttttgc agttgttcac ccgttggtgc
     3781 gggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg catccatttg
     3841 attaactcct gtcgtgatat ttattcacaa aattaacacg agagtggatt ttgttacagc
     3901 acagtccgca attcctgctg acaagtgccg gttgggtcat tacgataacc acatctattg
     3961 cgcctgtgac aggtgtgacc ttaagttggg agaatacatg cgaacccttt ggcgatttat
     4021 tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa tggtacttaa
     4081 cctgttcttt atttttctcg tgctggttgg tgtggggatc tggatgcagg tcagtggtgg
     4141 tgattcgaaa gaaacggcca gtcgtggtgc actgctgctg gacatttctg gtgtgatcgt
     4201 cgataaaccc gacagttctc agcggtttag taaattaagc cgccaactac ttggtgccag
     4261 ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aataccattc gccaggcgaa
     4321 ggatgaccgc aatatcaccg gtatcgtgat ggatctgaaa aacttcgcag gcggcgacca
     4381 accgtctatg cagtacatcg gcaaagccct gaaagagttt cgtgacagcg ggaaaccggt
     4441 ttatgccgtt ggcgagaact acagccaggg gcaatattat ctcgccagtt tcgccaataa
     4501 aatttggctg tcaccgcaag gcgtggtgga tttgcacggt tttgctacca acggtctgta
     4561 ctacaaatcg ttgctggata agctgaaagt ttccacccat gtgttccgcg tgggtacgta
     4621 taaatctgcc gttgaaccgt ttattcgtga tgacatgtca ccggcagccc gcgaagctga
     4681 cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg ctaaccggca
     4741 gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgagggtt taaccaaaac
     4801 cggtggcgat accgcgaaat atgcactgga aaacaagctg gtcgatgcac tggcatcgag
     4861 tgcggaaatc gaaaaagcac tgaccaaaga gttcggctgg agtaaggctg ataaaaatta
     4921 tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg gtgacagcat
     4981 cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag gaaactcagg ggaatgttgg
     5041 cggtgatacc actgcggcac aaatccgcga cgctcgcctt gacccgaaag tgaaagcgat
     5101 tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg tctgaagtga ttcgcgctga
     5161 actggcagca gcccgggcag cgggtaagcc tgtggttgta tcgatgggcg gcatggcggc
     5221 atctggtggt tactggattt ccacgccagc taattacatt gtggctaacc ccagcaccct
     5281 gaccggttct attggtatct tcggcgtgat caccaccgta gaaaatagtc tggattcgat
     5341 tggtgttcat actgatggtg tctcaacttc accgctggcg gatgtttcta tcaccagggc
     5401 actgccgccg gaagcgcagc agatgatgca gttaagcatt gagaatggct ataaacgctt
     5461 tatcacgctg gttgctgatg cgcgtcattc gacgccggag cagattgata aaatcgccca
     5521 gggccacgtc tggaccggtc aggacgcaaa agctaacggg ctggtcgata gtctcgggga
     5581 tttcgatgat gcggtcgcca aagcagcaga gctggcaaaa gcgaaacagt ggcatctgga
     5641 atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaacatgt ctggttctgt
     5701 ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgctgg cctcggtagc
     5761 ctctaccgtt aaaagtgaaa gcgacaagct ggcggcgttt aacgacccac aaaaccgtta
     5821 tgcgttttgc ctgacctgcg ccaacgtgcg ttaaatcctg caaaaatgtg gccgatatgc
     5881 tgtcggccac actcctgaat cggactgtct ttcactccct ctctccacta cattcccttg
     5941 ctcatttagc atctgatatg gagtcatcag tgaacaaggc cccctcttta attgccgcta
     6001 tcgtcctcgg actggggatt agcgcctgtg gttattttgt tggcgatggt gttaaacatt
     6061 taaaaaccaa caaccgttat gtcaatgtgc gcgggctttc tgaaaaagaa gttcgcgcag
     6121 atacggcgga attaacaatt gcgattaact ttaagggtaa tgttcccggc gaactgttcc
     6181 cgaaactgga agaagcgcag aaaaaaattg ttgccgaact caatgctcag gggattaacg
     6241 agaaagagat aattctgggc caatggacaa gcaagcgaac agattccttt tatctgaaag
     6301 atgatcccac aatgccacgc tataacgcag acgggtcagt gacgataaaa acccataatg
     6361 ttgctgctgt cgaaaaagtc gtcgcgaaat taaacgaact gcaagtcgct acggatggcg
     6421 ctatcgctga gagcaaagtc gcctatcgct ttaatggtat tggcgcactg cgcgctgaga
     6481 tgattgctgc cgccaccaaa gatgcacgca acgccgcact acaatttgca acggactcag
     6541 gtagccaggt ggggtcaatt agcgatgcct cacaaggcgt attccagatc tttgccagtg
     6601 gcagtgatga agatgatcct acggcgatta ataaaacggt acgcgtagtc acgaccgtaa
     6661 cctatgcgtt gcaggattaa ccacttttct ccggcgggac actaatccct ccgtcctgcc
     6721 ggacttttct catatccaca gagtatccag gtgcgctcgc tgcccctcac gtatatactt
     6781 ttgctctttc gatatcattc atatcaatat catgcaaaag aaatcaattt acgttgccta
     6841 cacgggcggg accatcggga tgcagcgttc cgagcagggt tatataccgg tgtcaggtca
     6901 tctacaacgc caactggcgc tgatgccgga attccatcgc ccggagatgc cagatttcac
     6961 cattcatgaa tatacgccgc tgatggattc ttcagatatg acgccggaag actggcagca
     7021 tattgctgaa gatattaaag cgcactatga cgactatgat ggttttgtca ttctgcacgg
     7081 caccgacacg atggcgtata ccgcctctgc gctgtcgttc atgctcgaaa atctcggtaa
     7141 accggtcatt gtgacagggt cacaaatccc gctggctgag ttacgctctg acggacaaat
     7201 taatctgctg aatgcgttgt acgttgcggc gaattatccg atcaacgaag taacactctt
     7261 ttttaataac cgactgtatc gcggcaaccg cactaccaaa gcccatgccg atggttttga
     7321 tgcgtttgcc tctccaaacc ttcctccgtt actggaagca ggtatccata ttcgtcgttt
     7381 gaatacgcca cccgccccgc acggtgaagg ggaattgatc gtacatccaa tcaccccaca
     7441 accaattggc gtagtgacga tttatccagg gatttctgct gacgtcgtgc gcaattttct
     7501 gcgccaaccg gtgaaagcat tgattctgcg ctcctatggc gtgggtaatg cgccacaaaa
     7561 caaagccttc ctgcaggaat tacaagaagc cagcgatcgc ggtattgtgg tggtcaacct
     7621 gacacaatgt atgtccggta aagtgaatat gggtggttat gccaccggta acgccctcgc
     7681 ccatgctggc gttattggcg gtgcagatat gactgtagaa gccacgctaa ccaaactgca
     7741 ttacctgctt agccaggaac tggatactga aaccattcgc aaggccatga gccaaaacct
     7801 gcgcggcgaa ctgacgccgg atgattaagg agactgcaat gccccctcgc gccctgttac
     7861 tggtcgattt acaaaatgat ttctgtgctg gtggcgcgct cgccgtgccg gaaggtgaca
     7921 gtacggtgga tgtcgctaac cgcctgattg actggtgcca gtcgcgcggt gaagcggtta
     7981 tcgccagtca ggactggcac ccggcgaatc acggcagttt tgccagccag cacggtgtag
     8041 agccttatac gccaggccaa ctcgacggtt tgccacaaac cttctggcca gatcactgtg
     8101 tgcagaacag tgaaggcgca caattacatc cgttactgaa ccaaaaagcg atcgcagcgg
     8161 tgttccataa aggcgaaaat cctttagttg acagttacag tgcctttttt gataacggcc
     8221 gtcggcagaa aacctctctc gatgactggt tacgcgatca tgaaatcgat gaattgatcg
     8281 ttatgggcct ggctactgac tattgcgtga agtttaccgt gctggacgcg ttacagttag
     8341 gttataaggt aaacgtgatt accgatggtt gtcgtggcgt gaatatccag ccccaggaca
     8401 gtgcgcacgc gtttatggag atgtcagcag ctggggcaac gctatatacg ctggcagact
     8461 gggaagagac gcaggggtaa ttttacgctg gcctacaatt ctgtactggc attgtaggcc
     8521 aaataaaaca cgtcagtggc acatctggca attgatgcca tcaacgaaag atttaattta
     8581 cctcagaaat ctcttccaac gacaccttcc gcgtttcgat gccaaaaatg gagagaacca
     8641 gcgcacagag caataacatg acaccaagta ccataaacac cgtgatcgac ccataatgtg
     8701 ttaataatgc cgcaacgcca tagggcgtga agactgcgac gatccgtccg acggcattaa
     8761 cgaaacccga accgcgcagg cgtaaatgcg ttggccaaag ctccgggata taaaccgccg
     8821 acgcgaagca aacatacatg tataaaaaga agatcatcac caggccatag attaaaatcg
     8881 cccactctgt agtctgaatt gaatagatat agcctaacac tgcaataata ataagtaagg
     8941 tggagccaaa taaccgacga ggaaaatgat caataattaa tgccgcaata aatattccta
     9001 ccggagcgcc aatcataata acagcggtca ttaatattga tttatcgaca tcaatgccgg
     9061 agttaacaaa tatggtcggt atccatacgg tgatggtata aagcgaaatg ttcattgcaa
     9121 ttaaaacagt aatcgcgact aatgtacgtc gtaacatttc acctttaaac aggagccaga
     9181 aagtaccctt gattacttta accttgctgt tgctctgata cgaagtaagc gggggtaaac
     9241 gaatactctt ctctctttca atttgctgct ctacttcacg aagttggcat tctgcacctg
     9301 cgatttgccc tttccctgcc agccatcgtg gcgactcaat aaagtattta cctgagagaa
     9361 accaggctaa cagtatgcca ataccaccca acagaaacat tatccgccaa ctaaaaaaag
     9421 cgataaccac cacacctatc gccgcagaca gcatgggcga ccagttacca acaaatgaga
     9481 gccgcgcgga ccattttcca cgcaccgtcg cggggataaa ctccgtaaat gaggcatagc
     9541 caaccataat cagcgccccc attcctgttc ccatcaggaa gcgaaagaag ataagccagt
     9601 acatatcagg gacaaaagcg gcccctgttg cagcaatacc gacgatgaga agatttatgc
     9661 gaaacgccct gcgccgccca aagtaatcac cgatgaaccc accggtaaga gagccgatga
     9721 aataaccaaa cattaatgcc gaggtaaagg cggcattgag gaaattattt gaccatccat
     9781 tgcttaccag ctttgctaag acgacattac cggagtaact caaaaaccc
//
LOCUS       DAZKFZ010000175         9732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000175 DAZKFZ010000000
VERSION     DAZKFZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            196..1509
                     /gene="gltP"
                     /locus_tag="ACHOQW_003704"
     CDS             196..1509
                     /gene="gltP"
                     /locus_tag="ACHOQW_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP9574705.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1687..2376)
                     /gene="yjcO"
                     /locus_tag="ACHOQW_003705"
     CDS             complement(1687..2376)
                     /gene="yjcO"
                     /locus_tag="ACHOQW_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP9574706.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2470..4617)
                     /locus_tag="ACHOQW_003706"
     CDS             complement(2470..4617)
                     /locus_tag="ACHOQW_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4198..4200),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFP9574707.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4815..6281)
                     /gene="mdtP"
                     /locus_tag="ACHOQW_003707"
     CDS             complement(4815..6281)
                     /gene="mdtP"
                     /locus_tag="ACHOQW_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP9574708.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            complement(6278..8329)
                     /gene="mdtO"
                     /locus_tag="ACHOQW_003708"
     CDS             complement(6278..8329)
                     /gene="mdtO"
                     /locus_tag="ACHOQW_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP9574709.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIHQGRDLLHAWDATWNSAQALDNALQPDKAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(8329..9360)
                     /gene="mdtN"
                     /locus_tag="ACHOQW_003709"
     CDS             complement(8329..9360)
                     /gene="mdtN"
                     /locus_tag="ACHOQW_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP9574710.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(9379..9654)
                     /locus_tag="ACHOQW_003710"
     CDS             complement(9379..9654)
                     /locus_tag="ACHOQW_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP9574711.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         2199 a         2582 c         2606 g         2345 t
ORIGIN      
        1 aaactcgcgt tattatgcat taatgcagcg aaaagctctg ttgttaaagg gttgcgcaac
       61 ataccgcgca aatgatactg atcataagcg ttaaaaaaat ctacaaacca acgcaacaca
      121 attcatgccc tggcagtatg tcacgttctc gcgtttctga acggggaacg gcgctccatt
      181 gaggaagtca ttcatatgaa aaatataaaa ttcagcctgg cctggcagat tctgtttgct
      241 atggtgctgg gcattctcct gggaagctac ctgcactacc atagcgacag ccgcgactgg
      301 ctggtcgtca atttgctctc tccggcgggt gatatcttca tccatctgat caaaatgatt
      361 gttgtgccga ttgtgatctc cacgctggtg gtgggtatcg cgggtgttgg tgatgccaaa
      421 cagctcgggc gtattggcgc gaaaaccatt atctacttcg aggtgatcac caccgttgcc
      481 atcattttgg ggatcactct ggcgaacgtc ttccagcccg gtgccggggt ggatatgtcg
      541 cagctggcga ccgtcgatat ctcgaaatat cagagcacta cggaagcggt acaaagcagt
      601 tcccacggca ttatgggcac gattttgtcg ctggtgccga cgaatattgt ggcgtcgatg
      661 gcgaaaggcg aaatgttgcc gatcatcttc ttctcggtgc tgtttggtct ggggctttcc
      721 tccctgcccg cgacgcatcg tgaaccgctg gtgaccgtgt tccgctccat ctctgaaacc
      781 atgtttaaag tgactcacat ggtgatgcgt tatgcgccgg tgggtgtgtt tgcgctgatt
      841 gcagtgacgg tggctaactt tggtttctct tctctgtggc cactggcgaa actggtgctg
      901 ctggtgcatt tcgccattct gttcttcgcg ctggtagtgc tgggaattgt ggcgcgccta
      961 tgcgggttaa gcgtctggat cctgattcgt attctgaaag atgagctgat tctggcgtac
     1021 tccactgcca gctctgaaag cgtactgccg cgaattattg agaagatgga agcctacgga
     1081 gcaccggcgt cgatcaccag cttcgtggtg ccgaccggtt actcttttaa ccttgatggt
     1141 tcgacgctgt atcaaagtat tgccgctatt ttcatcgctc agctgtatgg cattgacctg
     1201 tccatctggc aggaaatcat tctggtgctg acgctgatgg tgacctcgaa agggattgct
     1261 ggcgtgccgg gcgtgtcgtt tgtggtgttg ctggcaacgc tgggtagcgt aggtattccg
     1321 ctggaaggtc tggcgtttat tgctggtgtt gaccgtatcc tcgacatggc gcgtactgca
     1381 ctgaacgtgg tgggtaatgc gctggcggtg ctggtgattg ccaagtggga acacaaattt
     1441 gaccgcaaaa aggcgctggc ttatgagcgt gaagtgctgg gcaaatttga taaaactgcg
     1501 gatcaataat tgaagattgc cggggatatc ccccggcaat gtgtggatgc ctgatgcgac
     1561 gctggtgcgt cttatcaggc ctacgtcaga cagcgctagc gtgattttgt aggtctgata
     1621 aggcgtttac gctgcatccg acatcaacgc ctgatgcgac gcttaacgcg tcttatcagg
     1681 cctaccttaa ccgttagtta atttttcaaa ctcttcacac ccggtatcaa acccttccat
     1741 acagctcagg ttcaaccagt gcagcgcctt ttgcttgttc ttctcgataa aaccttcttc
     1801 accgtttaag aacatcattc ccgcccagta ctcggaataa ccggttcggg aaattgcaga
     1861 gctgcgtttg aaataccagg ttgccttgtc atcgtcggcc ttaatgccca cgccgttggc
     1921 gtaaatcaaa ccaagcagca tttgggcatc gacggcagag tcgttctcca gatcttccga
     1981 ggcgttttct aacagcgaaa tcgcctttgg ataatccggt ttacccgctt gagtatttac
     2041 cagaatatgc gccagcgtta cttcaccttc tttactgccc gcttgcgccg ctttctctgc
     2101 aagaactttt gcttgtggat agtccagact gaccggattg gtgattttga tctgcgccaa
     2161 cagcgcgcag gcatcagcat caccgctgtc ggcggctttc tgtgcccaat attcggcttt
     2221 gctcaaatcg ccggagctaa accagctgtc ggcaagaaaa tattgtgcgc gtcggtcccc
     2281 ggcctctgct gcctttaaat actggctgcc aggctcggag tcgttggcgt gggcaaagaa
     2341 tgtcaaaaac aacatcaatg cgataatttt tttcattttg ataatacgat tgggtaggga
     2401 gtaaccagta taaagagttc gaatggataa aaaaacagcc tccgaaagga ggctgtagaa
     2461 aggactgtat tacgccagtg ccgcttcgcg caggcgagtt ttcaacttgt tgtactcgtc
     2521 aatcacgtac tgctcggcgg cgcgctgatc ggcgatcggc tcgacgcgca cggcgcagta
     2581 tttgtattcc ggcgttttcg taatcgggct taagttttcg gtaaccagct cgttacaggc
     2641 accaatccac cactggtagg tcatgtaaat cgcccctttg ttcggacgat cgctgacctg
     2701 cgcacgggtg ataattttgc ctttacgcga gtgcacccaa accaatgcct catcttcaat
     2761 actcagacgt ttggcgtctt cggtattgat ttgtgcgtag ccaggttcat cagccagcgc
     2821 cgccagtgcc gcacagttac cggtcatcga acggcaagag tagtgaccaa cttcacgcac
     2881 cgttgacagt accatcgggt actcgtcggt gagtttgtcg attggcgcta cccagtcgca
     2941 ggtgaagaac tgcgccagac cgttcggggt gtcaaacttc tctttaaaca gataagaagt
     3001 cccctgatcg gcatctgaag tatcgcggca aggccactga atgaagccca gttcgcccat
     3061 tttctcgtaa gtcgcaccgt agaaatccgg gcacagatga cgcaactcat cccagatctc
     3121 ctgggtgttg ttgtagtgca tcggataacc catacgggtg gcgatttcac tgatgatttg
     3181 ccagtccgtt ttcagatccc atttcggttc aactgctttg aagaaacgct ggaagccacg
     3241 gtcagccgca gtaaacacgc cttcatgctc gccccacgac gttgacggta aaataacatc
     3301 cgccgccgac gcggttttgg tcataaagat gtcctgaacg ataaccagtt ccagatcttc
     3361 aaaggcttta cgtactgccg acagctccgc gtcagtttgt agcggatctt cgcccataat
     3421 gtacgcggca cgcacttcgc catgcgctgc gcggtgcggc agctcgctga tgcgataacc
     3481 ggtatgagca ggcaggcttt ccacgcccca ggctttggcg aatttctcgc ggttagccgg
     3541 atcttttacg tactggtatc ccggatacgt atccggcagc gcgcccatat cgcaggcacc
     3601 ctgcacgttg ttctgaccac gaaccgggtt aacacccgca tgtggcttac cgaggttacc
     3661 ggtcagcatc gcgaggctgg tcagagaacg cacggtttcc acgccctggt agaactgggt
     3721 tacacccatg ccccacagga tggcggcgct tttcgcctgg gcatacatcc gtgccgcctg
     3781 acgaatctca ctggcgctga cgccggtgat atcttcaacc gactccggcg tgtagccttc
     3841 aacgatttta cgatactctt caaagccttc tgtacgggaa gcgacgaatg ctttgtcgta
     3901 cagattttct tcaataatga catggcccat cgcattcaac agcgcgatgt tcgagccgtt
     3961 tttcagtgca atgtgcatgt cagcaatgcg cgcggtttca attttgcgcg gatcgcagac
     4021 gataattttc gccccgttac gtttagcgtt aattacgtga ttcgccacga ttgggtggga
     4081 atccgccggg ttgtacccga aaacgaacac taaatcggta ttatcaattt cgttaatagc
     4141 attgctcatt gcgccattac cgaccgattg gtgcagacct gcaaccgatg ggccgtgtca
     4201 gacacgagcg cagcagtcaa cgttattggt accaataacg gcgcgcgcaa atttttgcat
     4261 tacatagttg gtttcgttac ccgtaccacg cgaggagccg gtcgtctgga tggcatccgg
     4321 accgtacttc tctttgatgg cgctcaggcg ctcggcaacg taattcagtg cctcatccca
     4381 ggaaacaggt tcgagtttgc cgccacgctg gcgacggatc atgggggttt tcaggcgcgg
     4441 ggtcaggatc tgggtatcgt taatgaagtc ccagccataa taacccttca gacacagggt
     4501 accctggttg gttttcccct gcgctgcctc cgcccggacg attttgccgt tatcgacgac
     4561 caggttgatt ttgcaacctg atgcgcaata ggggcaaacc gtgacgactt ttttcatcgg
     4621 tctcgctcca gttaatcaaa tcacgcatac gcgctctcga ctacagtatg catcttttat
     4681 gccacatttt atttggggtc attccctgat attacgggca ctatttattc aaaactctga
     4741 cgaaaaacag gctgtcgtca gttttgacgt gacgaaacga aataccgcgt gacagccatc
     4801 acacggcaga cattttattt tttctcgacg acgggagccg cctgataccc gctgcccagc
     4861 gatttcatca actgaatgct ttggatcacc cggcggctgt ccagcatcag taatgacatc
     4921 tcttcggcaa gcaccggcaa ccgggcttcg gtagcctgta agcggctggt taagccgcgc
     4981 tgataggcgg cctcggcagc gcgctgggtg aagcgcgttg cttccacgcg ttcagcctgc
     5041 atttctcgct cgtcgttgag cgtttgcaga cgcgtgccgt tgacggcaac gtcacgcacc
     5101 gcgttcagta ctgactggtt gtaacgttca atcatcatgt tgctggcggc gcgagtgcct
     5161 tcgagattgg cattcaaccg tccaccgtca aacagcggca atttcagacc cgggatgaag
     5221 ttgaactggc gactggtttt tttgaataag gtatccaggt ggatggagtc cagaccgaaa
     5281 aacgctttga tatcaaagct tgggtagaac agcgcccgcg cggaatccac ctgatctaat
     5341 gacgcctgaa cataccagcg catggcttgc agatccgggc gtctggcgag caactcataa
     5401 gagagtgtcg ccggaatacc ggtctggact cgtggtaatg ccactggttt gatctccggc
     5461 atatcgctgg ctcccgcgcc aatcaatgca cgcagagatt ctcgcgtttc ggtgatttgc
     5521 cctttaacgg cagcaatttg tttatcgacc gccagaatct gtgcccgcgc gccgtggaaa
     5581 ggcacttgcg cttccagacc gtgcgccact ttactttggt gcgctttcac cgcgtaatca
     5641 atcacatcgc gagtttgttc taacagatcg agcatctgat agctggcctg catactgtaa
     5701 taaagctgcg ctacgcccgt ggtcagcgat agctctactg ctgcggtttc tgccagcgcg
     5761 gcattatgcg cgccaatggc ggcggtaacc gctgagcgat gcacacccca caaatcaaga
     5821 tccagtccgg caaacaaacc gactgtggct tccgtatagt acggcccgtc catacccagt
     5881 gctggcgcat ccatcgcata agggcttaaa aagccgttcg ccgagacgcg ttggcggttg
     5941 agcatcccta acgctgcgac ctgtaattgt gaaccggcat ctaacaaatc ggcctgggac
     6001 tgcgcttttt cttcccgcag tttcgcttcg gcgagggtgt gtgaaccact tagcgtccgt
     6061 tggatcagcg catccagctg cgggtcattg agttgtttcc accactgcgc ctgcggccag
     6121 ccggagctgg caagatgaat atcatcggcc agtttgattt gttccggttt gagctgttga
     6181 tgaggcgcag aatccttgcg taccagggca cagccggaaa tcagcgtcgt gctgccgaga
     6241 atgctgcaca acagcagacg tgaaagttga cgattgatca ttgcgtggct ccttgtgcct
     6301 gttccgtggc gggcgttaat gccggggctg tccagtctgg caagcgggcg aaaagctggc
     6361 agacgtgttg ctcctgtttt aataaggtgg gcaggatggc ctgcgaggcg ggtgtctctt
     6421 caagcgttat ttgaggagaa cggctgagtg cggttgccag accggcagcg tatttttcca
     6481 gggcgtcggc aaactgacct gctttgtccg gctgtagtgc gttatccagc gcctgcgccg
     6541 agttccaggt ggcatcccag gcgtgaagaa gatcgcggcc ctgatgaata accgtttgcg
     6601 aacgttcaat cagtaaggcg cgttcttcgc tgtccagttg acgctccagc gccacgcgtt
     6661 ggcacatctc ttcacaggca ttaaacgccg catgcagacc tatacgaatt tgcagataag
     6721 tgcgcagagc cgtgacttcc tgctggcgtg ggatccgcat tactttactt agcataccca
     6781 gcgcgccagc cagtttttgc ggcagcgtgc gcgcttcact ttcaggccag acaaaggtgt
     6841 aaatcaccgc agacaccacg gtaccaatga tgatacccag ggcgcgatcg cgaatttcca
     6901 ccaggtcgta caccggacca aaaacgtttt cgagcgtggc gagcgcgaag gtgaccacca
     6961 tctgtgtgcc gatataagaa gagcgttcag agctggtggc aatccatgcg cccaacagga
     7021 aaatcggtgc cagcacaaac agcaattcga caatattgtc cagccagggc atgaccagta
     7081 gcgtgaataa cagcgccaga atcgcgccgc aaaaggcccc gccaaaacgc agcaccatct
     7141 tctggtacga tgaaccgaca ttcggattag cgacgatcac gcaggtcagc atacaggtgt
     7201 gaatgccttc ccagtccacg ccgctgtaga aggtgtaaca gatcaaacag gcgagcagcg
     7261 tttttaccgc gtagcgcata tagtctggat tggtaaaagc atcagcgacc attgatggcg
     7321 gtttggcggc gggcgttggc ggcgtattcg ggtccatctg acccagttgt aacaacgtct
     7381 ggcagatatt ctccaggtta cattcccgtg ccgccatcgc ttcactttca ctgatccgcc
     7441 agtcgctttg ccagcactga ccttcggtaa tggcatgctg cagcttgttg atttctgaag
     7501 ctaatttttg tcggaattca ataattgcct gagaatcagc aaaagaggtg ggatcgtagc
     7561 gattcagcgt cgagtaaatg taggttaccg ttgccacgca gctttgccac catgcgcttt
     7621 gagttcgcca gttggcatcg tccgcgaggc aaaagacatt gagtttttgc agcgccagcg
     7681 cctctctttc aatccgcgtt tcgggtagcg gtgcgaggct gtccgtcagg tggctaatgg
     7741 catcatcaag ccgatcatta agcgcttgat gcatttgcga aatggcacga ctgggaaacc
     7801 acagtacgcc gattaacgtc attaataacg tcgggtagag gccaacaacg atacaccaca
     7861 gcgttaagcg cacgaccact tccggatagt cgagcatggc ggggaaggtt tgcccataaa
     7921 tggcgacaat ggcgacagcg aaaaagacca gtcccaaccg atgggtgcgc atcaaaaaca
     7981 tgcagcccat caggatcggc ccggcgatga tcaagcggat caacggttcg ccgtatgacc
     8041 atttgtagat caaaaacagg ctgccgatct ccagcaccgt ggcaaccaca aacaagatcg
     8101 cgacaaattt ggtgtaaaac gcgttcgact gaataccgta aaacagcact gccagcgata
     8161 acgccacaaa agggatctca aaagtcatcg agatcagaat caccagcagg cagcctaccc
     8221 agagttgcac ggtctgcggc acgcgacctg gtcgccgctc gcttaactct tcatgaaaga
     8281 acgccagcag cctgaccacc ggtaatggca gggagttgag cgcgctcatt attgcggctc
     8341 aagattagcg actgccgaag cgccgatgcg gaacatttcc gggtcaggtt tatcaaccat
     8401 gattttgacc ggaaaacgct gggcaacacg gacccagtta atagaacgag aaactttcgg
     8461 caggccgccc agcaccaggc cgccgtcatc cggtagcacg ccgtagccaa ttgaatccac
     8521 tttaccctcg aaagttttgc cgctgtcact catcaggcga atcgttgcag gtgtacctga
     8581 gcgaatattt ttcagatcgg tttcgcggaa gttggcgatc acgtaccagt gacgagtgtc
     8641 gattagggta aaaatagggc gcatggcaga agcaaattgc ccgacggagg ttttgaggga
     8701 aatgacccgg ccatcaaacg gtgcgcgaac ggtcgccatt tccagatgca gtttggtcag
     8761 ggcaatatcc gcttcgaccg ccgcacgctg ggcaactaat gcatccacgc cgctgacggc
     8821 gctggcggct gactgcgcct gcaacaatac agcattaaga tcggcttctg cagcgcgctg
     8881 cgcggtcctc gcacggtcaa cgtcttccgc tgagacaaaa ccttctttca gtaatggctc
     8941 ggtgcggcgt aatgtatctg tggcctgttt agcggcggca cgggcttttt ctaccgtggc
     9001 gttaaccgag tcggcaccaa actgttgcgc gtcaacgcta cgctgggtga gcataatttg
     9061 cttatccagt gccgcgaggg aggcttcaga tttcgccaga ttggcttcgt acgggcgcgg
     9121 gtcaatgcgg aacagcaaat cgccctgttt gactgcctgg ttgtcggtga ccgccagttc
     9181 tacaatgcgg ccgctgactt ccggcaccac atcaatggta tctgctgaca cgtaagcgtc
     9241 attagttgat ggcgcactgt ctacgcgcca gataacgaaa acaagggcaa ccagcgccaa
     9301 cgcaaccact aacagagcag ggaatttact gcgaggagct tttttcggcg tactttccat
     9361 gagcgcgtct caaatagatt agaaaaatgc cagccagaac agcatggcgt agagagcaaa
     9421 aagggcggta taaataattc cgacaaatgc cagcttgata tttgcccgct gaataactcg
     9481 cctcgtgatg agcgtcaata taaggctggc aatggcgcag aaaaaccagc tgggataata
     9541 agcgccgatc accgggattg ccggagataa tgagcatccc gaaaccatga agacgggaat
     9601 tatccaggct gtttttttga gttgcatcgc catgcgagag agaactgttg gcatattaaa
     9661 tacatcctgt ctgctgcgaa caatatctgt agtgaatatc aatatccatt gaatgcgtca
     9721 gtatggctat tt
//
LOCUS       DAZKFZ010000176         9731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000176 DAZKFZ010000000
VERSION     DAZKFZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            234..653
                     /locus_tag="ACHOQW_003711"
     CDS             234..653
                     /locus_tag="ACHOQW_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9574712.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            653..1921
                     /gene="umuC"
                     /locus_tag="ACHOQW_003712"
     CDS             653..1921
                     /gene="umuC"
                     /locus_tag="ACHOQW_003712"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP9574713.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1967..2497)
                     /gene="dsbB"
                     /locus_tag="ACHOQW_003713"
     CDS             complement(1967..2497)
                     /gene="dsbB"
                     /locus_tag="ACHOQW_003713"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP9574714.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2643..4184)
                     /gene="nhaB"
                     /locus_tag="ACHOQW_003714"
     CDS             complement(2643..4184)
                     /gene="nhaB"
                     /locus_tag="ACHOQW_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP9574715.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4406..5125
                     /gene="fadR"
                     /locus_tag="ACHOQW_003715"
     CDS             4406..5125
                     /gene="fadR"
                     /locus_tag="ACHOQW_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP9574716.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5177..6709)
                     /locus_tag="ACHOQW_003716"
     CDS             complement(5177..6709)
                     /locus_tag="ACHOQW_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP9574717.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7072..8370
                     /gene="dadA"
                     /locus_tag="ACHOQW_003717"
     CDS             7072..8370
                     /gene="dadA"
                     /locus_tag="ACHOQW_003717"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP9574718.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8380..9450
                     /gene="dadX"
                     /locus_tag="ACHOQW_003718"
     CDS             8380..9450
                     /gene="dadX"
                     /locus_tag="ACHOQW_003718"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP9574719.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
BASE COUNT         2424 a         2398 c         2605 g         2304 t
ORIGIN      
        1 aacaatgttt aatgtcatta tggcgaatgc ttctattcta ttttttagcc gggtgatatt
       61 tttcatttct gctggatgag cgtcgtcgcc agaaggccgc gtgagcacag gataagagaa
      121 cgaaaaatca gcagtctatg cagcgacaaa tattgatagc ctgaatcagt attgatctgc
      181 tggcaagaac agactactgt atataaaaac agtataactt caggcagatt attatgttgt
      241 ttatcaagcc tgcggatctc cgcgaaattg tgacttttcc gctatttagc gatcttgttc
      301 agtgtggctt tccttcaccg gccgcagatt acgttgaaca gcgcatcgat ctgaatcaac
      361 tgttgatcca gcatcccagc gcgacttact tcgtcaaagc aagtggtgat tctatgattg
      421 atggtggaat tagtgacggt gatttactga ttgtcgatag cgctattacc gccagccatg
      481 gtgatattgt catcgctgct gttgacggcg agtttacggt gaaaaaattg caactacgcc
      541 cgacggtaca gcttattccc atgaacagcg cgtactcgcc cattaccatc agtagtgaag
      601 atacgctgga tgtctttggt gtggtgatcc acgtcgttaa ggcgatgcgc tgatgtttgc
      661 cctctgtgat gtaaacgcgt tttatgccag ctgtgagacg gtgtttcgcc ctgagttatg
      721 gggtaaaccg gtggttgtgc tatcgaataa tgacggttgc gttatcgccc gaaacgctga
      781 ggcaaaggcg cttggcgtta aaatgggcga tccctggttc aaacaaaaag atctgtttcg
      841 tcgctgtggc gtggtttgct ttagcagcaa ttatgagctt tacgcagaca tgagcaatcg
      901 ggtgatgtcg acgctggaag aactatcgcc ccgcgtcgag atttacagta ttgatgaggc
      961 attctgcgat ctgacaggtg tgcgtaattg tcgcgatctg actgactttg gcagagaaat
     1021 tcgcgcaacg gtgctacaac gtacccatct tactgttggt gtggggatcg cccagaccaa
     1081 aacgctggct aagcttgcca atcatgcggc aaaaaaatgg cagcggcaga cgggtggggt
     1141 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
     1201 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
     1261 tctcgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
     1321 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
     1381 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgc atcacggatt atacgtcgat
     1441 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
     1501 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
     1561 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
     1621 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
     1681 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
     1741 caacgctccg cgccccggga gtgagcaatt gatggcggta atggatacac tgaatgctaa
     1801 agagggcaga ggaacactct attttgccgg gcaggggatc cagcaacaat ggcagatgaa
     1861 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
     1921 aacacagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
     1981 ggtcacgttt tttcgcttta aacggctggg aaatcaccac cagcaccgcg acaatcaggt
     2041 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
     2101 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     2161 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     2221 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     2281 ctacataacg cagtggcgtt tttggggcga tcgcgccaat cagcgcagca cccagaacgc
     2341 cgaataatgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     2401 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     2461 cccggccttg tgaacattgg ttcaaaaatc gtaacataat catttccctg caatatgcat
     2521 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     2581 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     2641 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     2701 aactcgacgc agagcagtcc gacgagtgtc aggacaaggg tgtaaggcag ggccatccac
     2761 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaacagg
     2821 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     2881 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     2941 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     3001 aaaatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     3061 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     3121 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgttcatc ggtgacaccg
     3181 gtcaatgatg ttgccagaat aatgactgac aaaccaatca agccaacttc cgccagatgc
     3241 aacgccagcg cagtcaccag ccagacgcca ataatcgcct ggacaatcag acggatttta
     3301 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gttgcaacac ttcgcggact
     3361 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctctaccag caggcaggtt
     3421 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     3481 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatt
     3541 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     3601 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggtg
     3661 tcttcggtac gggaagaggc tacgcgatga taaataccat aaaaaccgac ggcaacgctg
     3721 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc tgccgctacg
     3781 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     3841 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     3901 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     3961 accgcttcga tagccaacag gccaccgggg agcagcgggt agcatttcag ggccatcgcc
     4021 agagtgaaaa taaattccgc taccagcaac cagccagcga cgaaagggct gatgaggaaa
     4081 attaacggat ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     4141 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     4201 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     4261 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     4321 ttcttctttt tgtctgctat cagcgtagtt agccctctgg tatgatgagt ccaactttgt
     4381 tttgctgtgt tatggaaatc tcactatggt cattaaggcg caaagcccgg cgggtttcgc
     4441 ggaagagtac attattgaaa gtatctggaa taaccgcttc cctcccggga ctattttgcc
     4501 cgcagaacgt gaactttcag aattaattgg cgtaacgcgt actacgttac gtgaagtgtt
     4561 acagcgtctg gcacgagatg gctggttgac cattcaacat ggcaagccga cgaaggtgaa
     4621 taatttctgg gaaacctctg gtttaaatat ccttgaaaca ctggcgcgac tggatcacga
     4681 aagtgtgccg cagcttattg ataatttgct gtcggtgcgt accaatattt caactatttt
     4741 tattcgcacc gcgtttcgtc agcatcccga taaagcgcag gaagtgctgg ctaccgctaa
     4801 tgaagtggcc gatcacgccg atgcctttgc cgagctggat tacaacatat tccgcggcct
     4861 ggcgtttgct tccggcaacc cgatttacgg tctgatcctt aacgggatga agggactgta
     4921 tacgcgcatt ggtcgtcact atttcgccaa tccggaagcg cgcagtctgg cgctgggctt
     4981 ctaccacaaa ctgtcggcgt tgtgcagtga aggcgcgcac gatcaggtgt acgaaacagt
     5041 gcgtcgctat gggcatgaga gtggcgagat ttggcaccgg atgcagaaaa atctgccggg
     5101 tgatttagcc attcaggggc gataatccct tccgtttaaa aaacaaaccc ctcaaacgag
     5161 gggttttttg ttgtttttac agatttccca ttctcggcgg gcaacgttcc agcaactcga
     5221 cgctgccgtc ttcgttttgc tgttccagca tcacatcaaa tccccacagg cgatgcacat
     5281 gcttcagtac ttctttgcgc ccccgatcca gcggtgcgcg attatgtgga atgtaacgca
     5341 gcgtcagcga acggtcgccg cgcaaatcca cgttccagat ctgaatattc ggctccagat
     5401 tacttaagtt atattgcgac gatagccggt tacggatctc ccgataacct tcttcattat
     5461 gaatagcgga aatctccaga taattatgcc gatcatcatc cagcacggtg aagaagcgga
     5521 aatcacgcat cactttcggc gacaggaact ggctgataaa actctcatct ttgaaatcac
     5581 gcatcgcgaa atgcagcgtt tccagccagt cggaaccggc gatatccggg aaccagtatt
     5641 tgtcttcttc cgttggcgac tgacaaatcc gtttaatatc ctggaacatg gcgaacccga
     5701 gggcatacgg gttgatgccg ctgtaccacg ggctgttata ggggggctgg aagaccacat
     5761 tggtgtggct gtgcaaaaac tccagcataa aacgttccgt tactttccct tcatcataca
     5821 gatggttaag gatggtgtag tgccagaagg tggcccagcc ttcgttcatc acctgagtct
     5881 gtttttgcgg ataaaaatac tggctcacct tacgcacaat acgcaggatt tcacgctgcc
     5941 atgattccag cagtggcgca tttttctcca taaaatagag caggttttct tgtggttcgg
     6001 acggataacg gcgcgcttca gcaaccgttt tctcttcctc gcgcttcggc agggtacgcc
     6061 agagcatatt gacctgactt tgcaaatact cttcgcgact tttctgccgg gctttctctt
     6121 cttgcagcga gattttttgc gggcgtttgt accggtccac gccgtagttc atcagcgcgt
     6181 ggcacgagtc cagaagccgt tctacttcat caacgccata acgttcttcg cactcggtaa
     6241 tatatttacg ggcaaaaatc agataatcga caatcgaact ggcgtcggtc cagctacgga
     6301 ataagtaatt gtttttgaag aaagagttat gcccatagca ggcatgagcc atcaccagcg
     6361 cttgcatggt aatggtgttc tcttccatca ggtaagcgat acacgggtta gagttaatga
     6421 cgatttcata ggccagtcct tgctgaccgt gcttatacag ccgttcagtc tcgataaact
     6481 ttttaccgaa tgaccagtgc ggatagttaa ttggcatgcc gacgctggag taggcatcca
     6541 tcatctgttc tgaggttatc acttcaatct ggtgcgggta ggtatccagc cggtagagtt
     6601 tcgccacccg gtctatctct gccagataaa catccagcag gtcgaacgtc cagtcgggtc
     6661 catcgctcaa acgtgtggtg tccttattca tagaatcgat cgtcgccata cgcgcacctc
     6721 attgttgtcg gcgctctctg tgtggagcac ctcattgttg tcggcgctct ctgtgtggag
     6781 cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt
     6841 tgcgatttct tattatcaga gtgccactaa tccgtttctg aacggaattt tatgctggat
     6901 aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg
     6961 tgagccagct caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag
     7021 atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt
     7081 gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga
     7141 catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat
     7201 gccgggcaaa tctcccccgg atatgctgca ccgtgggcgg caccaggtgt gcctttaaaa
     7261 gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag
     7321 ttccagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa
     7381 aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc
     7441 gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc
     7501 gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg
     7561 tatcagctgc tggaatccag ccgcctggcg gaagtggagc ccgcgctggc agaagtagcg
     7621 cacaaactga cgggtggtct gcaattgccc aatgatgaaa ccggagactg ccagctgttt
     7681 acccagaatc tggcgcggat ggcggagcag gcgggggtta aattccgctt taatacgccc
     7741 gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg cgatgaagtg
     7801 attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc
     7861 attgttgata ttccggtgta tccgctgaaa ggctactcgc tgaccattcc aattgcgcag
     7921 gaagatggtg cgccggtatc caccattctt gatgaaacct acaaaatcgc cattacccgt
     7981 ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa taccgagctg
     8041 ttgcaaccgc gtcgtgaaac gctggagatg gtggttcgcg atctctatcc acgcggcggt
     8101 catgtcgagc aggcgacttt ctggacgggt ctgcgcccga tgacgccaga cggcacgccg
     8161 gttgtcgggc gtacacgctt taaaaatctg tggctaaata ccggtcacgg cacgctcggc
     8221 tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca
     8281 gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt
     8341 ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc
     8401 agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac
     8461 gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc acggtattga gcgtatctgg
     8521 agcgcgctcg gggctaccga tggctttgca ttacttaacc tggaagaagc aataacgtta
     8581 cgtgagcgcg gctggaaagg accgatcctg atgctggaag gatttttcca tgctcaggat
     8641 ctggaaatgt atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa
     8701 gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg
     8761 atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg tctggcagca gttgcgggcg
     8821 atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ccgaggcgga acatcctgat
     8881 ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aagggctgga gtgtcggcgt
     8941 tcgttgtcca attcggcggc gactctgtgg cacccagaag cgcattttga ctgggttcgg
     9001 cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatact
     9061 ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg
     9121 ggcgagcgtg tgggctacgg cggtcgttat actgcgcgcg atgagcagcg aatcggcatt
     9181 gttgccgcag gatacgccga cggttatccg cgccacgcgc ctaccggtac ccctgtttta
     9241 gtggacggcg tgcgcaccat gacggtgggg acagtctcga tggatatgct ggcggtcgat
     9301 ttaacgcctt gcccgcaggc gggtattggt acgccagttg agctgtgggg caaggagatc
     9361 aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcgctg
     9421 gcgttacgcg tcccggttgt gacggtgtaa cttgttgtaa gtcggatcgg aggcaacgtc
     9481 ttctggttgc aaaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac
     9541 agagccattg cccatgatag tgcccattaa aaggatggac actatttccc cggaacctga
     9601 actcaccgca caggcgttct acataaaacg cttacgcttc attgttgact cgaactcgac
     9661 ttcagataaa tcacgcttca cccttgatgc agattcgggt tcatgttcag aatgttgatt
     9721 aacaggctgc g
//
LOCUS       DAZKFZ010000177         9693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000177 DAZKFZ010000000
VERSION     DAZKFZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..773
                     /locus_tag="ACHOQW_003719"
     CDS             <1..773
                     /locus_tag="ACHOQW_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9574720.1"
                     /translation="GWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLIN
                     QLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGF
                     TASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIDIGLSGLLSPVLAIV
                     ALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLF
                     ASDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            799..1593
                     /gene="yfaU"
                     /locus_tag="ACHOQW_003720"
     CDS             799..1593
                     /gene="yfaU"
                     /locus_tag="ACHOQW_003720"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFP9574721.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHOQW_003721"
     CDS             complement(1634..2560)
                     /gene="rpnE"
                     /locus_tag="ACHOQW_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP9574722.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHOQW_003722"
     CDS             complement(2753..3943)
                     /gene="glpC"
                     /locus_tag="ACHOQW_003722"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP9574723.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHOQW_003723"
     CDS             complement(3940..5199)
                     /gene="glpB"
                     /locus_tag="ACHOQW_003723"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP9574724.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHOQW_003724"
     CDS             complement(5189..6817)
                     /gene="glpA"
                     /locus_tag="ACHOQW_003724"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP9574725.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7090..8448
                     /gene="glpT"
                     /locus_tag="ACHOQW_003725"
     CDS             7090..8448
                     /gene="glpT"
                     /locus_tag="ACHOQW_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP9574726.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHOQW_003726"
     CDS             8453..9529
                     /gene="glpQ"
                     /locus_tag="ACHOQW_003726"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9574727.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2210 a         2402 c         2681 g         2400 t
ORIGIN      
        1 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca
       61 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa ctagaaaaaa
      121 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
      181 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
      241 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
      301 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
      361 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
      421 tactggcggc gggcattgat attggtctgt ccgggctgct ttctccagta ctggcgatcg
      481 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
      541 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgcttg
      601 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
      661 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
      721 tcactctgcg tgtaaatcgc accgttgcgc agaccgacgt ggcacatcat taaataggtt
      781 aaggaacacg atgaacgcat tattaaccat ccctttaaag aacgtttacg caagggcgaa
      841 gtgcaaattg gtctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
      901 tccggttatg actggttgct gattgacggg gagcatgcgc caaacaccat tcaggatctt
      961 tatcaccagc tacaggcggt agccccctat gccagccaac ccgtgatccg tccggtggaa
     1021 ggcagtaaac cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     1081 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     1141 ggtgaacgtg gtgttggggc cagtgtagca cgggctgcgc gctggggacg cattgagaat
     1201 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     1261 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     1321 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     1381 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     1441 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     1501 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     1561 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     1621 gggactgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     1681 cgagcaccag gtcacgatca aagccctgtt ccagcatgcg caaggcaatt ttaatagctt
     1741 gttcatgcat gccttcctgc aaaccttcta atttaccttc ctgccaacca atttgatgtc
     1801 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     1861 cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     1921 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     1981 tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tcaagcagag
     2041 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtaatatcc accaacggaa
     2101 aagcttcggt gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga
     2161 gtgagtacgg gtagggcgag gtttcgccat gataaaacag caacggcacc accagcggga
     2221 ctctgtcata gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     2281 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     2341 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     2401 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     2461 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     2521 atactgcatc atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat
     2581 ttatgatgac gcgacattac ggttatccca ggcctttgac atctgccagt ttcagccttt
     2641 gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgc atacattggg cacggaatcg
     2701 tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca tcagaaagga gtttaagcca
     2761 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct
     2821 gccatttaca ggtttcgcag tcggtgacca ccagatctgc gccgctttct tctatctggc
     2881 ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     2941 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggatgttac
     3001 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     3061 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     3121 acagccagcg ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     3181 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     3241 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     3301 ctttatcggt aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca
     3361 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     3421 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct
     3481 gagccgccac gctgcgatac cagcgacgaa acgtgccgaa ggagtatttc ggtagcgtgc
     3541 ggcgatgatc gatttttaac gccgcatcaa gcaactgccg caccggtttc agcgaggtag
     3601 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatggctta
     3661 aaacaaaatt gcgcagcgac gggcgcgtgg tgtcatattt cgcccgtgcg cgctggatga
     3721 tatcgccaat cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat
     3781 atttcagtgc ctcgtcatac agtgcgccat cttttaaacg cagacgctcg ccatccggcc
     3841 cggcttgttt tggccctgga taaccgggat tcacccggct cactgggcag gcggtggtgc
     3901 agacggtgca cttaatgcag ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg
     3961 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     4021 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gtaaacaggt tttcgatggt
     4081 ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt acaccgaact gctgccacgg
     4141 ttgcggcgca aaaaaatctc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     4201 aaggccgaga atcggctctc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     4261 actgccgctg gcgagaaccg cgaaacgtgg acgcagcggg atatcggcgt gattgcgggt
     4321 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     4381 ccacacgcca ccctgacgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     4441 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     4501 acgccacagt ttgtcatcag ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     4561 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     4621 acgggcgata ttcaccgcgc ggaactcggt ggcgttattg cgcaggacat ccagttccgg
     4681 cagctctatt tctgcggttt caacggcaag gtcgagttca cgcaacgaag ctgccgcaag
     4741 gtgcgcctga aaatccatca gtccgctaat tcccactaca catattttct tcgcgggcag
     4801 cggccagacg gggacttctg gcgaacttag ccaggtagag cgcagagtgc cgagcggcgt
     4861 aatccgctgg tgaggcagtt ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa
     4921 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc ccgagaagag agtaaggatg
     4981 tgcaggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca cagcttgacc
     5041 atctggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     5101 acgcgtgaca atggcacagc gcaggccgtg tttttgcagc tgcaggccac agagtaatcc
     5161 ggcgaggccg ccgcccataa tgatagtatc aaagcgcatc tttctgctcc ttctccagac
     5221 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgca tctccccagg
     5281 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     5341 gcgcggacgt cgtgacgtta aaacgttgca gcagtccagc agcgcggcag gcgcagagtt
     5401 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     5461 cgtttaaatt ttctaccgcg tactgcactt cacccgcagt taccgcttcg cactcacata
     5521 ccaggctacg gtgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     5581 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     5641 cgggttcctg tgaacccgga agtgccagat cggcggtcgt acaggggcgc gtgttgccca
     5701 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     5761 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     5821 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     5881 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     5941 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     6001 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     6061 cggaaggttt acggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca
     6121 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     6181 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     6241 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     6301 catgagcggt aaggataaca gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     6361 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     6421 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     6481 cttcgcaggc gcgaataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa
     6541 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     6601 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     6661 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     6721 gcagcccgcg cagggcacag tcgcgggcaa ttccggctcc cgttgcgccg ccgccaatga
     6781 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     6841 tttaccattt agccatagta aaaacatgag ttgtttgatt tcgcgcatat tcgctcataa
     6901 ttcgaaagtg agacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     6961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     7021 aaacggcagg ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg
     7081 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg
     7141 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     7201 tatgctgctt actatttggt tcgtaagaac tttgcgctcg ctatgcctta cctggtggag
     7261 cagggattct cacgcggtga tttaggtttt gcgctttctg ggatctcgat cgcctatgga
     7321 ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc
     7381 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     7441 tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg
     7501 tggccgccat gcggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     7561 gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg
     7621 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     7681 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     7741 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
     7801 gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact gttgtggtat
     7861 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
     7921 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcttc
     7981 tatgagtatg cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc
     8041 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
     8101 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
     8161 ggcttcctga tctacggccc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
     8221 aaaaaagcgg caggaacggc agcgggcttt accggtctgt ttggttacct gggcggttcg
     8281 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
     8341 atggtaatga ttggcggcag cattctagcg gttatcttgt tgattgttgt gatgattggc
     8401 gaaaaacgtc gccatcagga attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
     8461 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
     8521 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
     8581 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
     8641 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attacctcga
     8701 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
     8761 cgcgatagat ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag gtttcgatat
     8821 tgaaaacggt aaaaaagtgc agacttatcc agggcgtttc ccaatgggta agtccgactt
     8881 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
     8941 gaaaaatatc ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
     9001 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
     9061 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
     9121 acccaaaatg ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga
     9181 aacgcagcag aaacagccgg acggaagctg ggttaattac aactacgact ggatgtttaa
     9241 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
     9301 gttgattgag gagacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
     9361 tcagcagaac aagctggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata
     9421 cacaactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggtt
     9481 gtttactgat ttccctgata aagcggttaa attccttaat aaagagtaat gctgctactg
     9541 caggatggcg ttcatgcgcc atcctctgct aaagcgaaat aatgctacta ccggttattc
     9601 ttttcattaa ccggtgcgtc ctgtaaaaaa gatgccaaca taattttcgt aaaactcatt
     9661 cgttaaaaaa gctaacactg ggcattgtta ttc
//
LOCUS       DAZKFZ010000178         9408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000178 DAZKFZ010000000
VERSION     DAZKFZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..290
                     /gene="ghoS"
                     /locus_tag="ACHOQW_003727"
     CDS             <1..290
                     /gene="ghoS"
                     /locus_tag="ACHOQW_003727"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9574728.1"
                     /translation="KNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIP
                     RQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            318..491
                     /gene="ghoT"
                     /locus_tag="ACHOQW_003728"
     CDS             318..491
                     /gene="ghoT"
                     /locus_tag="ACHOQW_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP9574729.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(610..2127)
                     /gene="lysS"
                     /locus_tag="ACHOQW_003729"
     CDS             complement(610..2127)
                     /gene="lysS"
                     /locus_tag="ACHOQW_003729"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9574730.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2364..3821)
                     /gene="dtpC"
                     /locus_tag="ACHOQW_003730"
     CDS             complement(2364..3821)
                     /gene="dtpC"
                     /locus_tag="ACHOQW_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP9574731.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(3880..6027)
                     /gene="cadA"
                     /locus_tag="ACHOQW_003731"
     CDS             complement(3880..6027)
                     /gene="cadA"
                     /locus_tag="ACHOQW_003731"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP9574732.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(6107..7441)
                     /gene="cadB"
                     /locus_tag="ACHOQW_003732"
     CDS             complement(6107..7441)
                     /gene="cadB"
                     /locus_tag="ACHOQW_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP9574733.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7807..9345)
                     /gene="cadC"
                     /locus_tag="ACHOQW_003733"
     CDS             complement(7807..9345)
                     /gene="cadC"
                     /locus_tag="ACHOQW_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP9574734.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         2653 a         2193 c         2129 g         2433 t
ORIGIN      
        1 gtaaaaacaa gttcaatact tatgttgttt cttttgatta tccatcatct tattcctcag
       61 tgttcttaag gttaagatca ttgatgtatg atatgaattt ctcttctatc gtggctgatg
      121 aatatgggat accacgacaa ttgaatgaaa attccttcgc aataacgaca tcgttagccg
      181 caagtgaaat cgaagattta atcaggctta aatgcttaga cttaccggat attgactttg
      241 acctcaacat tatgacagtt gatgactatt tccgtcagtt ttacaagtag ccagtaagga
      301 taagaagaac ataaaaaatg gcactattct ctaaaatatt aattttttat gtgattggtg
      361 tgaacatatc ctttgtcatt atctggttta tctcacatga gaaaacacat attcgtttac
      421 ttagtgcatt cctggtcgga ataacctggc caatgagtct gcctgtggca ttactttttt
      481 ctctctttta ggagctgtag ttgagtggac ccgctggtta tcgtgaaacg aagcgtctct
      541 cctcaaaact ggatttgcgg aacgatgaac gaagcagtcg cctgactgct tcgttcatta
      601 aagtgaaatt tatttctgtg ggcgcatcgc cgggaagaga ataacgtcgc gaatagtatg
      661 gctgttagta aacagcataa tcattcggtc gataccaata cccagaccag cggttggcgg
      721 cagaccatat tccagcgcag tcacgtaatc ttcgtcatag aacatggctt cgtcgtcacc
      781 tgcggcttta gcgttaacct gttcctggaa acgttcagcc tgatcttctg cgtcgtttaa
      841 ttcgctaaaa ccattaccga tttcacgacc accgatgaag aattcaaaac ggtcggtgat
      901 ttccgggtta acatcattac ggcgtgccag cggtgacact tctgccggat attccgtaat
      961 aaaggttggc tgaatcagat gtgcttctgc cacttcatca aagatctccg tgacaatacg
     1021 tcccagcccc cagcttttct ctaccgtaat accgatagat tcagctaatg ctttagcagc
     1081 atcaaaatta tccaggtcgg ccatatcggt ttctggacga tattttttga ttgcttcgcg
     1141 catggtgagt ttttcaaacg gtttgccgaa atcaaacaca tgctcgccat aagtgacttt
     1201 agtggtaccc agaacctctt gtgccagggt gcggaacagg gactctgtca gttcaatcaa
     1261 atcgtggtaa tccgcatacg ccatgtagag ttccatcatt gtgaactcag gattatggcg
     1321 aacagaaata ccttcattac ggaagttacg gttgatttcg aatacccgtt caaaaccgcc
     1381 tacaaccaga cgtttcagat acagctccgg cgcgatacgc agatacatat ctaaatccag
     1441 agcattatga tgggtaataa acgggcgagc agatgcccca cctggaatta cctgcatcat
     1501 cggggtttct acttccataa agccgcgcgc gaccatgaac tggcggatag cggccagaat
     1561 ttttgaacgg acaacaaacg tttgacggga tttatcgtta gcgatgaggt ccagataacg
     1621 ttgacgataa cggacttcct gatcctgcag accatggaat ttatctggta aaggacgtag
     1681 tgctttagtc agcaggcgca actcagtaca gtgaatggaa agctcacccg tttgcgtctt
     1741 aaacagcgta ccgcgggcac cgataatgtc acccagatcc cattttttaa actgatcgtt
     1801 ataaacacct tctggcaggc tatctcgtgc gacgtacagt tgaatacggc caccgacatc
     1861 ctgcaacgtt acaaaggagg ctttccccat gatacgacgg gtcatcattc ggccagcaac
     1921 cgagacttca atgtttaagg attccagttc ctggttatcc ttcgcatcaa actcttcgtg
     1981 caactggtca gaggtatggt cgcggcgaaa atcattggga aacgccacac cttgctgacg
     2041 tagtgccgcc agtttttcgc ggcgatttct cagttcatcg ttaaaatcaa tagcctcatt
     2101 ggctccccgt gtttcttgtt cagacatttt ggttcctcta aatccagctt tcaatttagc
     2161 gtagataaag agacagatcg gtacactgta ttatctgcct cgactattaa taactcagtt
     2221 tatcttatcg aaactaacgc ttacctcaag ttgatatcac taataaataa ccatcaaaat
     2281 ggtttaaatg acttattaat ctcgatttac tagtgtgata aactgaaagt taatttattc
     2341 ataaccaatt gtttttacga cagttaatcg ttgctctcct gtaccatatt cgtcggcgtg
     2401 ctaaacagga aacgggtagc aaaggccaat accacgatga tcgcgacaca agccaacgtc
     2461 cactctccca tctgagaaaa gaatcgctgg taagctgcaa ttgccatacc gctaatttgc
     2521 gactctgtcg tctgctgtgc cactacgcct gccagccagt tggcgaccgc gcctgttgcc
     2581 agcatataaa tcccggttaa tacgccagac attttcagac gcgtgatttg cgcaatcgcc
     2641 accgggtcaa taaagagttc ggcaaagccc attagcgcca gcccggatac catcacgccc
     2701 attgacgctt gaccgtcagc tgctgcatgt cgggcatcaa atgccagcaa cataaagcca
     2761 caagccatca gcagtaagcc aaaggcaaac ttcagccaga cgcgcaatgt tgagttgccg
     2821 tggctttctg gcgacgccag ccaggccaga acaaccccag cgagcatcac cgcaattgca
     2881 ttcaccgact ggaatagtgc agtaggtact tcaatattga atgcctgacg attcacaaag
     2941 cgatcgataa acaagctgat ggtactgccg ccctgttgtg ccagtaccca gaacaatgtc
     3001 ccgacaaaca tcaacaatac aatttgccaa agagcacggc ggtgttcggg gaatttgatc
     3061 atcatgcggg caatgatttg tgcggcaatg aggcaaacga tcgccagcaa atatccggac
     3121 cagtcgttct ccagcagcag ggtaaaaaat actggggcta aacagagcat caccactaac
     3181 cagctccata ccggtaaggc aaatttgacg ctagtgagcg cttttttatc catactacgc
     3241 gtggactgga aatgacgatg cccgcttaag aaaatcaaca aaccgataaa catgccgcca
     3301 cccgcaaggg caaagccaac atgccatcca taccactgag cagccaggcc gcaggcgatt
     3361 ggggcggcga tagaaccgat attgcccgca gcatacagca gcgaaaaacc gccatcacgt
     3421 cgatgatcgt tctcgtcgta gagctcgcca agcaaacagc tgatgtttga tttgaataaa
     3481 ccgtagccac aaataatgat tgccagcgcc agatacaggc taaaggttga atttgtatca
     3541 atacccagca ccacatggcc aagggtcatt aacagcgcgc cggcaatcac tgcagtgcgg
     3601 ttgccgagca ggcggtcggc aagccagccg ccgagaatag gggtaacgta aaccagagaa
     3661 gcatatgcgc tgaacaggct gatggcatgg ttatcatcaa aaccaagctg atgggtgaga
     3721 tagagaatga gtaaggcacg catgccgtaa aaactgaagt actcccagat ttggatcgcc
     3781 acgatatagt atatcgcgcg cggctgtgag ggtgttttca tgtgttctcc ttatgagcaa
     3841 aaaagggaag tggcaagcca cttcccttgt acgagctaat tattttttgc tttcttcttt
     3901 caatacctta acggtatagc ggccatcagc ctgacggtat gcaccgtgaa tatcggtttc
     3961 aaagcccgga tagtgagcgc cgatttcaca cagcatctgc aggaactcca gaaccggacg
     4021 gctttcttcg gtgatcattt cacccggcat taccagagga actcccggcg gatacggaag
     4081 gatcatattg gcgttaatac gaccgaccat ttcgtcgagg taaacttctt cggtcatacc
     4141 gtgcagctct ttctggaatg cagcatacgg agtcattacc atcgtcggca gcacttcaaa
     4201 tgcgcgatac atcagatccg gcagattgtg gtgaacaatc agtttgtgga tattctgagc
     4261 cagttcctga atacgcatgt tttcatagaa ttcaggatct tcacgataca gagacggcag
     4321 catgtttttc acacgcaggt tcaggtcgaa cgcacgtttg aagtcagtca gagcacgcag
     4381 caggctcagt gctttggtct tatcgatacc gatgctgaac aggaacagca ggttatacgg
     4441 accggttttc tcaacaacga tgccatgttc gtcgaggtat ttcgccacga tgctggccgg
     4501 aataccaaag tcgctcatgg tgccgtcttt ttccatcccc ggagtcagca gggtgacttt
     4561 gatcgggtca agatacatgt gctcgttatc gatgtttttg aagccgtgcc aggtgctgtc
     4621 agaacgcagc ggccagcatt cagtcgtatc gatatgatcc ggctgccata catcaaagaa
     4681 ccagccatca gattccgttc tcagacgttt gatctcttta cggaatttga tcgcacgttc
     4741 aatggaaccg ttgatcagac gcttacctgc attgcctttc atcatcgccg cagcggtttc
     4801 agtggacgcc acgataccgt agtgcggaga agtggtggtg tgcatcatgt aggcttcgtt
     4861 aaaggtttct tcgtttacgt cacctttaac gtggatcatg gaagcctgag agaacgccgc
     4921 cagcagtttg tgagtggact gggtttcgta aatcactttc ccttctacac ggccaccgct
     4981 cataccgcat ttaccttcgt aaatcggtga gaagttggtg taaggcaccc acgcggagtc
     5041 aaagtggatg gatttcacat ccagtgtttt cttgatgaag tcggtgttgt acagcagacc
     5101 atcataggta gagttggtaa ttacagcatg taccggccag gttgcgtttg gtgtttcttt
     5161 cacgcgctta gcaatggtag cgtgctggaa ttcactctgt gggataccac caagaatacc
     5221 gtaagcgtta cgggtcgggc ggaaatagat tggcgtaaca tcgctcatca tcatcaggtg
     5281 ggtcagcgat ttgtggcagt tacggtcaat cagaatggtg ctgcctgccg gagcagagta
     5341 cataccaaca attttgttcg cagtggaagt accgttggtc accatgtagc tgcggtctgc
     5401 gttaaagacg cgagcgatat actgttctgc ttctttgtgt ggaccactgt gatccagcag
     5461 agaacccagt tcagatactg aaatggaaat atcagatttc atggtattcg gaccaaagaa
     5521 atcatagaac aggctaccta ccgggctttt ctggaatgca gtaccaccca tgtgaccagg
     5581 agtacagaaa gtatatttac cttcacgaac atatttaaac agtgctttag tcagcggagg
     5641 cagaatagtg ttgatatatt cgtcagtggt ctgcttgatc ttgttagcaa tatcttcagc
     5701 agcacccagc gcatattcaa agaagctaat ctgtaaacgc aggtcattca ggcttacatc
     5761 gagagtggaa tacgtattag cgaacgcgta caacggcagg ttctcgttca ttttgctaat
     5821 ttcttcgcac agctcgagat tatatttatc ccagtcaaaa ataacgccgc acagacgcgc
     5881 attgttttcg atcagtttta ataagtcgtc acggtcgttc gggtaaacaa tctggaagtt
     5941 cagacgttca agcgcgcgat gaagttcacg gatgggttct tctttaaaat aaacccccat
     6001 gtgattcaat attgcaataa cgttcatagt catatctcca ggtaaaaaag gcccctccca
     6061 acacatggga caaaatgaaa ggaggagcct cggaaaatac ttttaattaa tgtgcgttag
     6121 acgcggtgtg gttatccatt gagtggctct ggcgctcgtg cattttgcga gcgtagaaca
     6181 tcaggataat caggctgacg atgaaggtac ctgccagctc gaaggagctt gcgcccatca
     6241 gcgcgatgaa gcagaacacg caacccagta cagagcagat caggctgaca aagttgcgga
     6301 tgttaacgcc ttcaaaacga atcaggtcaa cgcaagagta gaagtacggc agcatagtca
     6361 gcagtactgc gataccggtc agttcaccga acaggtcaga tgctttacca ccggcagagt
     6421 tcatcagagt gatgaggatc atcagggcag tcattttcac tgcagccagc agcagacctt
     6481 ttttcggaat accgttgctg tcgacttcac cataaacttt cgggaagtta ccgtcgttag
     6541 cggcacgtac acctgcctgg cctaccaaca tcatccagga gcccagagaa gtcaggcacg
     6601 caaaggcggt gaatgcagaa accagcggcg cagcccagtt accgaggata gttgaagcac
     6661 tgattgcaaa cggagcaccg gaagccgcca ttacagaaga cggatacata ccggaaagca
     6721 cctgagtcgc agcgatgtaa acaatacctg ctaaaccagt acccagcatg gttgccagcg
     6781 gaacggtacg tttcgggttt ttaaccatac cagtacttac agctgcggat tcaacaccca
     6841 cgaaggccca caggcagagc agaatacttt taatgatcgc atgaccatca gtggtatccg
     6901 cagtattcca gttagctgca taagttgccg catcaaacca atgccagcca acaatagcag
     6961 tcatcaccac aggaataaga accagcacca gaccaatagt ggttaaacgg cttacccagg
     7021 taccgccgag catatttaca aaggtaaata cccagacgat agcaatacag gcgatacccg
     7081 ccggaacagg atcatttaat actgggaaga aggtggaaag ataagataca gcggtaatac
     7141 caatcgccag gttaccaatc cagttagcat ggtaataaag aacacctgtc tgaaaaccaa
     7201 atgcaggaga aatttctccg gcataagcaa ttgggccacc ttgttgcggg ttttttgttg
     7261 ccagtcgggc atatacatac gccagcgaca ttgcaccaat aatagagata atccaacccc
     7321 agatagcaat accaccgata cttgctaggt tcgcaggtaa taatgcaata ccgctcccca
     7381 tcatattacc ggcaacaaca ccggtacagg caaatagccc gatcttcttg gcagaactca
     7441 tgctcttctc ctaatttcat ttttgaattt ggagtccggg tcatgatgta taactatttc
     7501 ctgaccagac caaactggcg ctaagattac tcacgaaaaa aggattaatc ctaaagatta
     7561 ggtgaaataa cacaaaagtt tctgtaagtg agaacttgag gttttttatt aacacatcag
     7621 gatcgcaagt tgatatcatg aaaagataaa cattcaatgt ttacaataga ttgcgtgacg
     7681 tcttctggtt aaatttatgt aataaaaatt atgcggcaaa taaattgccg caacatatta
     7741 taccaacagg aacatacaaa aactcaacaa caaatattcc cgagcatgaa tcaacccggg
     7801 ggttacttat tctgaagcga gaaatttgtc gagataaggt acaacataag gaacagaagt
     7861 ctggaatata ccattttcaa tccagtaaag ggtgtttgcc cctgggcgta aattaaaggc
     7921 ggtgagatat gcatcagccg cttcccggtt catccccttc atttcataaa ccttgccaag
     7981 caatacataa tttagccagg acatttcaag atcaatgcca gtatttatcg cctggtaaga
     8041 ctcatctgtt ttacctttta ccagagcact gaccgctttt atttgatata taatggacag
     8101 gttattcaat tccggtagtg taacaatgtt atctatttct gtgttcagtg ctgctaattg
     8161 tttttcatct aaaggatgtt gagaatggcg cacgatatca actaatgctt tttctgctct
     8221 cgcgtaggta aattctgggg atgattgaac aatctcacct aataattcac tggcacggtt
     8281 caatgattta tcatcgccat gcagtaaata atcatgtgcc tgataaaaat tagttaataa
     8341 cgcaccacga tgaggcaaaa tttgctggag cgtctcctgc attcgttgtg gccacggttg
     8401 gtttaacgct tttgataaac tctccagtaa atcattttga atcgccagct gattaccgtt
     8461 agtgatgaca taacgtttat ccagcatggt tgaaccatct gcattgtcta ccaattttat
     8521 cgacataaag cattgttgag cacggtattg gcgctgatta acaaacgcaa tagataatgt
     8581 tttaccggaa ctgctcggtt catcaatgtt gtagttgatt ttgtcatgca ccataaaggt
     8641 ggagaaggtg ttaagtgatg tcgccaccaa atcacccacg cctatcgcgt aagagagctg
     8701 atacggggaa ctccagctgt tacaactttt atttaccata ttaatgtcaa tatcgcgtgg
     8761 attgagcaaa atacgcgatt tgctcatcgg aagacgtgta tcaagacttg aaaacgctac
     8821 cagtgctaca cagataccta aggacaacag gaaaaaaaac catacccaaa aggtagtgaa
     8881 tcgtttgctt ttaactgggg attgttcagg tggcgttgcg gtgttttgaa tgttaagact
     8941 gtgggaggga gaatctgtgg caggaaccgc ctctggtata gggggaggcg aagatagcat
     9001 tatttcctct ccctcttctt cgctgtacca gataaccggc accattaatt tatagccgcg
     9061 ctttggtaca gtagcgatat agacaggact atcttcatca ttatctttta atgacttacg
     9121 tagttctgag atactctgcg tcacaacgtg attggtgaca atacttctct tccagacatt
     9181 atcgataagt tcatccctgc taagtacttc gccactgtgt tgagcaaaga aaaccagaag
     9241 gtcgattaat ctcggctcaa gggtaagttg acgcccattg cggctaattt ggtttatgga
     9301 cggagtaaca agccattcgc caacgcgaac tacaggttgt tgcataatag aaactcattc
     9361 gaaaagggaa tgatgcaatg ataattgcca caacctattt ttaccatc
//
LOCUS       DAZKFZ010000179         9402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000179 DAZKFZ010000000
VERSION     DAZKFZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(148..2139)
                     /gene="tkt"
                     /locus_tag="ACHOQW_003734"
     CDS             complement(148..2139)
                     /gene="tkt"
                     /locus_tag="ACHOQW_003734"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9574735.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2417..3175
                     /gene="loiP"
                     /locus_tag="ACHOQW_003735"
                     /gene_synonym="yggG"
     CDS             2417..3175
                     /gene="loiP"
                     /locus_tag="ACHOQW_003735"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP9574736.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3381..4301)
                     /gene="speB"
                     /locus_tag="ACHOQW_003736"
     CDS             complement(3381..4301)
                     /gene="speB"
                     /locus_tag="ACHOQW_003736"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP9574737.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4437..5168)
                     /locus_tag="ACHOQW_003737"
     CDS             complement(4437..5168)
                     /locus_tag="ACHOQW_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9574738.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5314..7290)
                     /gene="speA"
                     /locus_tag="ACHOQW_003738"
     CDS             complement(5314..7290)
                     /gene="speA"
                     /locus_tag="ACHOQW_003738"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP9574739.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7299..7430)
                     /gene="yqgB"
                     /locus_tag="ACHOQW_003739"
     CDS             complement(7299..7430)
                     /gene="yqgB"
                     /locus_tag="ACHOQW_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP9574740.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(7524..7667)
                     /gene="yqgG"
                     /locus_tag="ACHOQW_003740"
     CDS             complement(7524..7667)
                     /gene="yqgG"
                     /locus_tag="ACHOQW_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP9574741.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(7778..8029)
                     /gene="yqgD"
                     /locus_tag="ACHOQW_003741"
     CDS             complement(7778..8029)
                     /gene="yqgD"
                     /locus_tag="ACHOQW_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP9574742.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8085..9239
                     /gene="metK"
                     /locus_tag="ACHOQW_003742"
     CDS             8085..9239
                     /gene="metK"
                     /locus_tag="ACHOQW_003742"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9574743.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2325 a         2526 c         2383 g         2168 t
ORIGIN      
        1 atatcgtgat gcatattaca taattgatat ttattgcata taaatatttg cgatctacaa
       61 cacgctttat ctattgcttg tccgcaaacg gacatatcaa ggtaataaaa aaggtcgccg
      121 aagcgacctt tttacccgaa atgctagtta cagcagttct tttgctttcg caacgacgtt
      181 gtcgacggtg aagccgaact cttcgaacag caactctgcc ggagctgact caccgaaggt
      241 ggtcataccg atgatagcgc cattcaggcc aacatacttg taccagtagt cagcaatacc
      301 cgcttctacc gcaacgcgtg cagtaaccgc tttcggcagt acggattcac ggtaagccgc
      361 atcctgcttg tcgaatgcat cggtagacgg catggaaacc acgcgcgcct tcacgccttc
      421 ggcagtcagt ttttcgtagg cggcaacagc cagttcaact tccgaaccgg tcgcgatgaa
      481 gatcagttcc ggctgaccag cacagtcttt cagcacataa ccaccgcgcg cgatgtttgc
      541 cagttgctct tcagtacgtt cctgctgcgc caggttctga cgggagagga taagcgcggt
      601 cggaccgtcc tgacgctcaa caccgtattt ccacgcgacc gcggattcaa cctggtcaca
      661 cggacgccat gtagacatgt tcggggtcac gcgcagagaa gcaacctgct caaccggctg
      721 gtgagtcggg ccatcttcgc ccagaccgat ggagtcgtgg gtgtaaacca taacctgacg
      781 ctgtttcatc agcgcagcca tacgtacggc gttacgtgcg tattcgacaa acatcaggaa
      841 ggtggaggtg tacggcagga agccaccgtg cagggagata ccgttagcaa tcgcggtcat
      901 accgaactcg cgaacaccgt agtggatgta gttacccgca gcatcttcgt tgattgcttt
      961 agaaccagac cacagggtca ggttagacgg tgccaggtca gcggagccgc cgaggaattc
     1021 cggcaacagc gggccgaacg cttcgatagc attctgagac gctttacggc tggcgatttt
     1081 cgccggatta gcctgcagtt tagcgataaa ctctttcgct ttagcgtcga agtcagacgg
     1141 catttcgcct ttcatacggc gggtaaattc agccgcttcc tgcggataag ctttcgcgta
     1201 agccgcaaac ttctcattcc atgcagattc tttcgcctgg cctgcttctt tcgcatccca
     1261 ctgcgcatag atttcagacg ggatttcgaa cggtgcgtat ttccagccca gctgttcgcg
     1321 ggtcagagcg atttctgcat cacccagcgg cgcaccgtgg gagtcgtgag taccggcttt
     1381 gttcggggaa ccgaaaccga tgatggtttt gcacatcagc agggacggtt tgtcagtcac
     1441 tgcgcgcgct tcttctactg cgcgtttgat ggatgctgcg tcatgaccgt cgatgtcgcg
     1501 aataacgtgc cagccgtaag cttcgaaacg cattgcggtg tcgtcggtga accagccttc
     1561 aacgtgacca tcgatagaaa taccgttgtc atcgtagaac gcaatcagtt tacccagctt
     1621 cagcgtaccc gccagagagc aaacttcgtg ggagatgcct tccatcatgc agccgtcgcc
     1681 catgaaggcg taggtgtagt ggtcgacaat gtcgtggccc ggacggttaa actgcgccgc
     1741 cagcgttttt tctgcaatcg ccatacctac tgcgttggca ataccctgcc ccagcggacc
     1801 ggtggtggtt tccacaccag cggtgtaacc cacttccggg tggcccggag ttttagagtg
     1861 cagctgacgg aagtttttca gttcttccat cggcagatcg taaccggtga ggtgcagcag
     1921 gctgtagatc agcatggagc cgtggccgtt ggacagcacg aagcggtcac ggtcagccca
     1981 ggacggattc tgcgggttat gtttcaggaa atcacgccac aggacttcgg caatgtcagc
     2041 catacccata ggggcacccg ggtgaccgga tttggctttc tgtactgcgt ccatgctcag
     2101 cgcacgaata gcattggcaa gctctttacg tgaggacatt ttgactccag atcggatgat
     2161 gaagggcacg cccttaacga cttgacgaca gcgcgttttg ggctacgccg gaaaatttgc
     2221 caacaattta ccgcaagccg cgcgtcatgt acatggaaca tccttttgcc gcttcagaaa
     2281 tctctggatc atgctcgcat gttgcgcaat ctactcgccc gtccgctgcg cttttcctta
     2341 tactgagact gagcgtcggt tcacctgcaa acggcgcatt tttagaataa tcctgacctt
     2401 gtgcggaaga aaaaacatga aaattcgcgc cttattggta gcaatgagcg tggcaacggt
     2461 actgaccggt tgccagaata tggactccaa cggactgctc tcatcaggag cggaagcttt
     2521 tcaggcttac agtttgagtg atgcgcaggt gaaaaccctg agcgatcagg catgtcagga
     2581 gatggacagc aaggcgacga ttgcgccagc caatagcgaa tacgctaaac gtctgacaac
     2641 tattgccaat gcgctaggca acaatatcaa cggtcagccg gtaaattaca aagtgtatat
     2701 ggcgaaggat gtgaacgcct ttgcaatggc taacggctgt atccgtgtct atagcgggct
     2761 gatggatatg atgacggata acgaagtcga agcggtgatc ggtcacgaaa tggggcacgt
     2821 ggcattaggc catgtgaaaa aaggaatgca ggtggcactt ggtacaaatg ccgtacgagt
     2881 agctgcggcc tcggcgggcg ggattgtcgg cagtttatct caatcacaac tcggtgatct
     2941 gggcgagaaa ttagttaatt cgcaattctc ccagcgccag gaagcagaag ccgatgatta
     3001 ttcttacgat cttctgcgcc aacgcggcat cagcccggca ggtcttgcca ccagctttga
     3061 aaaactggca aaactggaag aaggtcgcca aagctcaatg tttgacgacc atcctgcatc
     3121 cgccgaacgc gcccagcata ttcgcgatcg catgagcgcg gatgggatta agtaaagcct
     3181 ggtggtgtcg aacgtaggtc agataaggcg ttcacgccgc atctgacatg aatggcacat
     3241 ttgttacctt gtgcgcattg ccggatgcga tgctggcgca tcttatccgg cctacgggtg
     3301 ccgaacgtag gtcggataag gcgttcacgc cgcatccgac attaatggca cgttttaccc
     3361 gtgcgcatcg catctggtgc ttactcgccc tttttcgccg cctgaatata cagcatttcc
     3421 agcgccagcg ttgccgctgc cagagcggtg atttccgact gatcgtatgc cggagccact
     3481 tccactacgt ccatcccaac gatgttgaga tctttcaggc cgcgtaccag tttaatagcg
     3541 cgatcggagg tcagaccgcc aattaccggc gtaccggttc ccggcgcaaa tgcaggatcc
     3601 aggcagtcga tatcaaaggt caggtaaacc ggcatatcac ccacaatttg tttcacctgg
     3661 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     3721 ttgtctttat caaattcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     3781 ccttctttcg gcgcggtata gaacatggtg ccgtggtcaa attcacaacc gttcgcgtag
     3841 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     3901 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg agagcatacg cttaccggca
     3961 gccagcagct tctcggcgtg cgcctgcagc ttctcgctca tctcacgggc atcgccgaaa
     4021 gcatatacca gatcaccaca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
     4081 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatagc tgccggaccg
     4141 tggcgtcccc ccgcacgacc agaagtggcc atatcgaacg gcacaccagt aatcacccag
     4201 tcagcatcgc tgtcatacgg ctggaagttc atcggcaggc gcaaaaaacc aaaggcgttg
     4261 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     4321 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgcg
     4381 aaacacaagt ttgtgctaat tatcgcctta aatgggcgag gcatcaagcg aggttgctat
     4441 ttccgacacg gtggcggcgc atagccattg ttatctgccg gaaaggcttg tccagagcac
     4501 ggtggcagcg cgttttgcgc ctggccttgt aattcaaagg cggcaaagcc gattacaccg
     4561 gtattacgca catcgccttg cgccgaattg gctgcgtagg aatcagcaac gttggcgaac
     4621 tgaaaagccg cttcggtatg tttatctttg cggaatcctt taatcgctaa cgaatctccg
     4681 gcattcacaa tatagccgtt gttattcagc gaaccttgct tgccgttcag cacatccagg
     4741 ccatcaaccg tagcaacgat ctcgtagttg gtgttacgac tgtaattacg gacatataat
     4801 tgataattca ttccgactct ggcatggaca atatagcgtg gttcattatt agatgaatca
     4861 taaacacgcg taataggtag agaattaaaa ttaccatcgc gaatagcata ttccagttcg
     4921 ttaatacgaa tgctataaac gcgatcgtag ccggaaggaa cattagtcga ataactaata
     4981 acgatcatct ccgccggatc gcgccaggct cgctcagcag atacgccctg aaccgttgag
     5041 tgcacatccc caccccaggc ggtgccgaca ttgcccgatg cactgctatt atactgcgca
     5101 ttactggaac aaccggccag cagcacgatt aatgccacca acgcgctgcg tactttccat
     5161 tttttcataa ccgttccctc tgcatttaat taatgcagcg attgtaggga gattatttaa
     5221 taagaaaagc agctaaatta tttttaagaa tatataaaaa gaaagcggac cgatttacgt
     5281 aaataaaccg acccgccagc agaataatct taattattca tcttcaagat aggtataacc
     5341 gtacaaacct gcctcgaact cttcaaggaa ctgttgttgc agttcagcat caagatcggt
     5401 tttcttcact tgatcacgga actgagttaa cagcgttttc ggatcgagct gtacatattg
     5461 cagcatgtcc gccacggtat cgccttcgtc agacagttcc acttctacac taccgtcagg
     5521 gaagacgaac acgtcaaccg cttcggtatc accgaacagg ttgtgcatgt tgccgaggat
     5581 ctcctgatat gcgccgacca taaagaaacc gagcatcggc ggattctctg gatcgtactc
     5641 cggcataggc attgtcgtgg caataccatc accatcgata tagtggtcga tagcaccgtc
     5701 agagtcacag gtaatatcca gcagcacagc gcggcgttcc ggcacttgat ccagcccttc
     5761 cagcggcaga accgggaaca actggtcgat cccccacgca tccggcatcg actggaacag
     5821 cgagaagttg acgtacattt tgtccgccat acgttcctgc agctcgtcga taatcggacg
     5881 atgagcacgg ttttgcggat ccagctgctt ttgcacttca tggcacatgc tcaaataaag
     5941 ctgctcagcc catgcacgtt cttgcaggct aaaggtgccg gaagagtagc cgatatgaat
     6001 gtcgtgcaga tccatctgac tgtcgtgtaa ccattcacgc agagaacggc gagttcccgg
     6061 ttcgtgcatc tcctgccagg tttcccacat gctttgcagc gcgcgcggcg catcttctgc
     6121 aggcgcggtc ggcaccgtgt attcgttacg ttccacgccg atgatattag acaccagcac
     6181 ggtgtgatgc gcagtcactg cacgacccga ttcggtgatt accgtcggat gcggcagacc
     6241 gttttcttca cacgcatcac caatcgccca gatgatgttg ttggcatatt cattgaggcc
     6301 gtagttcacc gaacagtcgg actgcgaacg agtaccttca taatccacgc ccagaccgcc
     6361 gccgacgtcg aagcactgaa tattgacgcc cagcttgtgc agctcaacat agaaacgcgc
     6421 ggattcacga acgcctgtcg cgatatcgcg aatattcgcc atctgcgaac cgaggtggaa
     6481 gtgcagtagt tgcaggctgt cgagacgccc ggcttcacgt agggtttcaa ccagttgcag
     6541 tacctgagtc gccgccaggc cgaacttcga tttttccccg ccggaggact gccatttacc
     6601 ggagccctgc gaagccagac gtgcacgcac gcccagacga ggaacgacat tcagacgttc
     6661 tgcttcatcc agcacaatgg cgatttctga catcttctca atgaccagat agaccttgtg
     6721 ccccatcttc tcgccaatta atgccaggcg gatatattcg cggtctttat aaccattgca
     6781 gacgatgacg ctacgggtca tgccagcatg tgccagcact gccatcaact cggctttgga
     6841 accggcttcc agacccagcg gttcgcccga atgaatcagg gactcaatca cgcgacggtg
     6901 ctggttaact ttgatcggat aaacaaggaa gtaatcgccg ttatagccgt aggattcccg
     6961 cgcacgtttg aacgcggcgt taatggaacg caaacggtgc tgcaggatct gtgggaaaca
     7021 gaacagtgca ggcagacgct gaccctgtgc ttcacgagtt ttcactaact gcgcgagatc
     7081 gacgcgagct tccgggacgt ccgggtccgg gcacacgctg atgtggccca gctcgttaac
     7141 gtcatagtag ttattgcccc accaggcaat attgtaagta cgcagcatct tgctggcttc
     7201 ctgggagctc attgcaacct cctgcatgga gcgtagtaca ccgtgttcgc ccgctgacga
     7261 aggcaaaccc atagacatgt cgtcagacat agcgaacctc aaattatttt attaagtgta
     7321 aaacagttaa cgactatcgc agcctggaac tgcgataaca acccataagc acatggattt
     7381 tccagcagtg aatgctgacg ctccaactgc gcaaccggtt tctttttcat aacattatta
     7441 agcacataac cgaacgtaag tgtgacagtt cggcgaaacc acgagaaaac tcttgttttt
     7501 acaagagcgc ccttgttcag tcctcagtaa ctgtaaccag ctcttgaatc ctgagaagcg
     7561 ccgagatggg tataacatcg gcaggtatgc aaagcagaga tgcagagtgc ggggaacgaa
     7621 tcttcaccag aacggtgaga caggttaagc agcagacaac ggttcattat ttcgtatcac
     7681 ctccacggcc gcctgttaag acgaacccac aagccaaaac tctgatttca acccggctgg
     7741 aagtggcaac acgaaagaaa cgtcgtgtgc tttttattta agccgcgcgc cgcgttttat
     7801 accccacaat ggcagaaaat tgcaaaagat aaatacgcag aatgccggca ttgtcaggaa
     7861 aaatttccag ccacgttttt aacagaatga gacacgattc aaaaaaaagt ggaaatcggg
     7921 tgaagaattg acctaaaata gccatccaga tgttaatcca tccataccga ttaacactca
     7981 gactgccagt gtttttaacc tgcagagtcg tggtaggatc cgctaccaca gaaaatccac
     8041 acaacagttt gagctaacca aattctcttt aggtgatatt aaatatggca aaacaccttt
     8101 ttacgtccga gtccgtctct gaagggcatc ctgacaaaat tgctgaccaa atctctgatg
     8161 ccgttttaga cgcgatcctc gaacaggatc cgaaagcacg cgttgcttgc gaaacctacg
     8221 taaaaaccgg catggtttta gttggcggcg aaatcaccac cagcgcctgg gtagacatcg
     8281 aagagatcac ccgtaacacc gttcgcgaaa ttggctatgt gcattccgac atgggctttg
     8341 acgctaactc ctgtgcagtt ctgagcgcta tcggcaaaca gtctcctgac atcaaccagg
     8401 gcgttgaccg tgccgatccg ctggaacagg gcgcgggtga ccagggtctg atgtttggct
     8461 acgcaactaa tgaaaccgac gtgctgatgc cagcacctat cacctatgca caccgtctgg
     8521 tacagcgtca ggctgaagtg cgtaaaaacg gcactctgcc gtggctgcgc ccggacgcga
     8581 aaagccaggt gactttccag tatgacgacg gcaaaatcgt tggtatcgat gctgtcgtgc
     8641 tttccactca gcactctgaa gagatcgacc agaaatcgct gcaagaagcg gtaatggaag
     8701 agatcatcaa gccgattctg cccgctgaat ggctgacttc tgccaccaaa ttcttcatca
     8761 acccgaccgg tcgttttgtt atcggtggcc cgatgggtga ctgcggtctg actggtcgta
     8821 aaattatcgt tgatacctac ggcggcatgg cgcgtcacgg tggcggtgca ttctctggta
     8881 aagatccatc aaaagtggac cgttccgcag cctacgcagc acgttatgtc gcgaaaaaca
     8941 tcgttgctgc tggcctggcc gatcgttgtg aaattcaggt ttcctacgca atcggcgtgg
     9001 ctgaaccgac ttccatcatg gtagaaactt tcggtactga gaaagtgcct tctgaacaac
     9061 tgactctgct ggtacgtgag ttcttcgacc tgcgcccata cggtctgatt cagatgctgg
     9121 atctgctgca cccgatctac aaagaaaccg cagcatacgg tcactttggt cgtgaacatt
     9181 tcccgtggga aaaaaccgac aaagcgcagc tgctgcgcga tgctgccggt ctgaagtaat
     9241 ctttcttcac ctgctttcaa aggccagcct cgcgctggcc tttttctttt acctcaccgc
     9301 ggtggatagg cgttcacgcc gcatccggca aaaaaaccgc ctgcacaata acatcattct
     9361 tcctgatcac gtttcaccgc aaattatcat cacaactgaa ac
//
LOCUS       DAZKFZ010000180         9365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000180 DAZKFZ010000000
VERSION     DAZKFZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..63
                     /gene="tnaC"
                     /locus_tag="ACHOQW_003743"
     CDS             <1..63
                     /gene="tnaC"
                     /locus_tag="ACHOQW_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP9574744.1"
                     /translation="HICVTSKWFNIDNKIVDHRP"
     gene            284..1699
                     /gene="tnaA"
                     /locus_tag="ACHOQW_003744"
     CDS             284..1699
                     /gene="tnaA"
                     /locus_tag="ACHOQW_003744"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP9574745.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1790..3037
                     /gene="tnaB"
                     /locus_tag="ACHOQW_003745"
     CDS             1790..3037
                     /gene="tnaB"
                     /locus_tag="ACHOQW_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP9574746.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3169..4344
                     /gene="mdtL"
                     /locus_tag="ACHOQW_003746"
     CDS             3169..4344
                     /gene="mdtL"
                     /locus_tag="ACHOQW_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP9574747.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4319..5278
                     /gene="yidZ"
                     /locus_tag="ACHOQW_003747"
     CDS             4319..5278
                     /gene="yidZ"
                     /locus_tag="ACHOQW_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP9574748.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5435..6184
                     /gene="yieE"
                     /locus_tag="ACHOQW_003748"
     CDS             5435..6184
                     /gene="yieE"
                     /locus_tag="ACHOQW_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP9574749.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6206..6772
                     /gene="yieF"
                     /locus_tag="ACHOQW_003749"
                     /gene_synonym="chrR"
     CDS             6206..6772
                     /gene="yieF"
                     /locus_tag="ACHOQW_003749"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP9574750.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6826..8163)
                     /gene="adeP"
                     /locus_tag="ACHOQW_003750"
     CDS             complement(6826..8163)
                     /gene="adeP"
                     /locus_tag="ACHOQW_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP9574751.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8329..8994
                     /gene="yieH"
                     /locus_tag="ACHOQW_003751"
     CDS             8329..8994
                     /gene="yieH"
                     /locus_tag="ACHOQW_003751"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP9574752.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9131..>9365)
                     /locus_tag="ACHOQW_003752"
     CDS             complement(9131..>9365)
                     /locus_tag="ACHOQW_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574753.1"
                     /translation="FDNKRDNWNSIEVQDEVNALNAKIVDCDDKIRSKLTIVRNNRLE
                     NQYKDDKNISDAMRNILDWFERYPDIVQNITQA"
BASE COUNT         2304 a         2337 c         2317 g         2407 t
ORIGIN      
        1 catatatgtg tgacctcaaa atggttcaat attgacaaca agattgtcga tcaccgccct
       61 tgatttgccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatctatt
      121 tgtttgctat atcttaaatt tgccttttgc aaaggccatc tctcgtttat ttacttgtgt
      181 tagtaaatga tggtgcttgc atatatacat ggcgaattaa tggatattgc agatgcaata
      241 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
      301 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacca ctcgcgctta
      361 tcgtgaagag gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
      421 gtttatcgat ttactgaccg acagcggcac cggggcggtg acgcagagta tgcaggccgc
      481 gatgatgcgc ggcgacgaag cttacagcgg cagccgcagc tactatgcgt tagccgagtc
      541 agtgaaaaat atctttggtt atcaatacac cattccgact caccagggcc gtggtgcaga
      601 gcaaatctat attccagtac tgattaaaaa gcgcgagcag gaaaaaggcc tggatcgcag
      661 caaaatggtg gcgttctcta actatttctt tgataccacg cagggccata gccagattaa
      721 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
      781 ctttaaaggc aactttgacc ttgagggatt agaacgcggt attgaagaag ttggtccgaa
      841 taacgtgccg tatatcgttg caaccatcac cagtaactcc gcaggcggtc agccggtttc
      901 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tatgatattc cggtggtaat
      961 ggactccgcg cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
     1021 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
     1081 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
     1141 tttctttgat gtgtacaccg agtgcagaac cctttgcgta gttcaggaag gcttcccgac
     1201 gtatggcggc ctggaaggcg gcgcgatgga gcgtctggcc gtaggtctgt atgacggcat
     1261 gaatctggac tggctggctt atcgtattgc gcaggtgcag tatctggtcg atggtctgga
     1321 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
     1381 actgcttccg catatcccgg cagatcagtt cccggcacag gcgctggcct gcgagctgta
     1441 taaagtcgcc ggtatccgcg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
     1501 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
     1561 atatactcaa acacatatgg acttcattat tgaagccttc aaacatgtga aagagaacgc
     1621 gtcgaatatt aaagggttaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
     1681 aaaactgaaa gaagtttaat taatactact gagtggctat aaggatgtta gccactctct
     1741 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
     1801 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaatcgg
     1861 tggaggtatg tttgctttac ctgtggatct tgccggtgcc tggtttttct ggggtgcctt
     1921 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
     1981 tttaaattat cccgtcggct ccagttttaa caccatcacc aaagatttaa tcggtaacac
     2041 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
     2101 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gttatcacgc
     2161 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
     2221 ctcgttagcc gccagtcgta ttacctcatt gttcctcggg ctgaagatta tctcctttgt
     2281 gatcgtgttt ggttctttct tcttcctggt cgattactcc attctgcgcg atgccaccag
     2341 ctccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
     2401 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
     2461 taagctaatc aaaagcgtgg tcttcggttc gctgctggcg ctggtgattt atctcttctg
     2521 gctctattgc accatgggga atattccgcg agaaagcttt aaggcgatta tttcctcagg
     2581 cggcaacgtt gattcactgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
     2641 gttttgcctg ctggtgttct ccaacttagc tgtcgccagc tcgttttttg gtgtcacgct
     2701 gggattgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
     2761 caaaaccgtg ctgttaacct tcctgccacc cgcgttgttg tatctgatct tcccgaacgg
     2821 ctttatttac gggatcggcg gtgccggact gtgcgccact atttgggcgg tcattattcc
     2881 cgcagtgctg gcaatcaaag ctcgcaaaaa gtttcccaat cagatgttca cggtctgggg
     2941 cggcaatctt attccggcga ttgtcattct ctttggtata accgtaattt tgtgctggtt
     3001 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagtcattc
     3061 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc ctgatcctcc
     3121 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
     3181 ttgatttgta gttttgccct ggttttactt tatcccgctg ggattgatat gtacctcgtt
     3241 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttaca tattgcgttc
     3301 tccgtttatc tggcaggtat ggcagctgcg atgttatttg ccggtaaagt ggccgatcgt
     3361 tcagggagaa aaccggtcgc catccccggc gcggcgctat ttattatcac ttcggtgttc
     3421 tgttcactgg ctgaaaccag cacgttattt cttgcagggc gctttctaca ggggttgggc
     3481 gcgggctgtt gttacgtggt ggcattcgcc attttgcgcg acacgctgga tgatcgacgt
     3541 cgggcaaaag tgctgtcgtt actcaacggc attacctgca tcattccggt gttagcgccg
     3601 gtgctcggtc atctgattat gcttaaattc ccgtggcaga gtctgttctg gacgatggca
     3661 ataatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgccctgcc
     3721 gcccccgcag cttcggacaa atcacgagaa aatagcgagt cgctgcttaa tcggtttttc
     3781 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacctt cgtcaataca
     3841 tcgccggtat tgctgatgga aatcatgggt tttgagcgcg gagaatacgc caccattatg
     3901 gcgttgaccg ctggcgtcag catgaccgtt tcattttcca cgccatttgc gctgggaatt
     3961 tttaagccac gtacgttgat gatcacctca caggtgttat tcctggcggc gggcatcact
     4021 cttaccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
     4081 ttctcggtag gttttggtgt ggcgatgagt caggcgttag ggccgttttc attacgcgcg
     4141 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
     4201 ctggcagcgg tggttggtat cagcgcatgg aatatgctga tcgggattct gattgcctgt
     4261 agcatagtga gcctgttgct gattatgttc gtcgcgcctg gacgcccggt taccgctcat
     4321 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcagc tgctgatgca
     4381 ggagcgcagc gtaaccaaag cggcgaagcg gatgaacgtg acaccttcgg cggtgagtaa
     4441 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgttggg
     4501 gctgtcgccc acaccgctaa tggtcagcat ggagcaaaat ctggcggagt ggatgcaaat
     4561 gagcaacctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagct
     4621 ggcggcagaa tcaccgctaa tgatgatcat gcttaatgcg ctgtcgaaac agatctacca
     4681 acgttacccg caggcgacca tcaaattacg taactgggat tacgattcct tagatgccat
     4741 tactcgtggt gaagtggata tcggtttttc cggtcgcgaa agtcatcccc gttcgcggga
     4801 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ttgtttagtg atgtgccctg
     4861 cgtctggtta cgcaaagatc atccggcact gcatgaaaag tgggatctcg ataccttttt
     4921 acgttatccg catatcagca tttgctggga acagagcgat acctgggcgc tggacaatgt
     4981 gttacaggag ctggggcgcg aacgtacgat tgcaatgagc ctgccggaat tcgagcagtc
     5041 actgtttatg gcagcgcagc ccgacaatct gctcctggcg accgcgccgc gctactgtca
     5101 gtactacaat caactccacc aactgccgtt ggttgctctt cctctcccgt ttgacgaaag
     5161 ccagcaaaaa aagctggaag tcccttttac cctgctgtgg cataaacgga acagccataa
     5221 tccgaagatc gtctggttac gggataccat taaaaatctt tacgcgtcga tggcataacc
     5281 gaatcgtatg aaacgcgacc cgatttcaca ataaaatgta aaaaagttgt aataagcttg
     5341 tctgaatcga acttttagcc gctttagtct gtccatcatt ccagtaaatg attactcttg
     5401 tattcataat ggaccattaa gcatggagcg aaaaatggcg actcactttg cccgagggat
     5461 tttaacggaa ggacatctga tttctgttcg tctcccctcc cagtgtcatc aagaagcccg
     5521 aaacattccc cctcatcgtc aaagccgttt tctggcgtcc agaggtttac tcgcagaact
     5581 gatgttcatg ctgtatggca ttggcgaatt gccggaaatc gtcaccctgc cgaaaggtaa
     5641 accggttttc agtgataaaa atttgccttc gttttctatt tcctatgccg ggaatatggt
     5701 tggcgtggcg ttaacaaccg aaggtgaatg tggcctcgat atggaactgc agcgtgcgac
     5761 gcgcgggttt catagcccac acgcgcccga taaccacaca ttttccagca atgaatcgct
     5821 atgggtcagt aaacaaaatg atccaaacga agcgcgggcg cagctcatca cgctgcgccg
     5881 aagcgtgcta aaactaaccg gtgatgtttt gaatgacgat ccgcgcgatc tgcaactgct
     5941 gcccattgcc ggacgcctga aatgtgctca tgtaaatcat gtagaagcgt tatgcgacgc
     6001 ggaggacgtg ctggtgtggt ccgtggcggt cacgcccacg attgaaaagc tcagtgtctg
     6061 ggagttagat ggcaaacacg gctggaaaag cctgccggat attcacagcc gcgccaacaa
     6121 tcctaccagc cggatgatgc gttttgccca gctctctacc atgaaggctt tttcgccaaa
     6181 ttgatagaca accacaggag tcattatgtc tgaaaaattg caggtggtta cgttactggg
     6241 gagcctgcgc aaaggctcat ttaatggcat ggttgcacgt accctaccga aaattgctcc
     6301 ggcgagcatg gaagtcaatg cgttaccttc cattgccgac attcccctgt atgacgctga
     6361 cgtacagcag gaagaaggtt ttccagcaac ggttgaagct ctggcggaac agatccgtca
     6421 ggctgacggt gtggtgatcg tcacgccgga atataactat tcggtaccgg gtgggctgaa
     6481 aaatgccatc gactggcttt cccgcctgcc ggatcaaccg ctggccggta aaccggtgtt
     6541 gattcagacc agctcaatgg gcgtgattgg cggcgcgcgc tgtcagtatc acctgcgcca
     6601 gattctggtt ttcctcgatg caatggtgat gaacaagccg gaatttatgg gcggcgtgat
     6661 tcagaacaaa gttgatccgc aaaccggaga agtgattgat cagggtacgc tggaccacct
     6721 gaccgggcaa ttgaccgcat ttggcgagtt tattcagcga gttaagatct aaataaaaaa
     6781 cccgccagca atcatgcatg gcgggttttt aacgcgctat cgattttagt gagcgtcgat
     6841 aaatacaatc ttcaggataa acagcagcgc aacgatgatt acgcacgggc taaggtcacg
     6901 aatacgcccg gtaccaatct tcatcacgca gtaggagata aagcccagcg caataccttc
     6961 ggtaatcgag aagctgaacg gcatcatcac agcggtaata aacgccggaa cagattcagt
     7021 aagatcctgc cagttcacgc gagcaagact tgaggtcatc aacacgccaa cgtaaatcag
     7081 cgcgccagct gcagcgtagc ctggcaccat tcccgccagc ggcgacagaa agataaccag
     7141 caggaacagc agaccaacaa ccactgccgt cagaccggta cgaccgccaa ccgatacgcc
     7201 ggaagaggac tcaatataag ccgtaacgga agaagtaccg ataaacgaac cggtcacgga
     7261 agagatactg tcgacataca gcgcctgctt catgcgcggg aatttcccct tctcatctgc
     7321 cagaccggct ttatcggtca cgccaatcaa cgtgccggag gagtcaaaca ggttgaccag
     7381 catgaaagag aaaatcaccc ctgccagccc gaggttaaac gacccggcta aatctacatg
     7441 acccacgaca gtcattacgc tcggcggcgc agaaacgatg ccattgtagt ggacatcacc
     7501 cagcatccag cccagcagcg ttgtcaccac gatagaaacc agcaccgctg cgtgaatgtt
     7561 gcgtgaggcc agaatagcaa tgatgaagaa gccgaggatc cccagcagca cgctgtgaga
     7621 agtcagatta ccgatgctga ccagcgtttc cgggttagcg acaatcacac ctgcgttttt
     7681 cagccccatc atgccaatga acagaccgat accgctggta atgcccacac gcagactcac
     7741 cggaatgttg gcaatcatcc agtagcgaac gcggaaaatc gtcagtaaca gcagaccaat
     7801 cgcgccccag aagattgcgc ccatcccgac ttgccacggc aagcccatcg cctgcacaac
     7861 gacaaaagcg aagaacgcat tcaggcccat agcgggtgcc agtgcaactg gcaggttagc
     7921 aaacagcccc atcataatac tgccgaatgc agcaatcaga caggtagtga cgaagacggc
     7981 gctggtatcc atgccagcaa cgccaagaat ttgcgggtta acaaaaacga tgtaaaccat
     8041 cgtcaggaag gtggtaaaac cggcgatcac ttcggtccgt gccgtcgtgc catgttcgcg
     8101 cagtttaaac acgcgttcca gcatcccctg gccagaagcc tgggttgtgt gttgttgact
     8161 cattatctat ttccgaacaa ggagggaaaa tccgtcgcta tcgtatacca aaatgagaca
     8221 atatggacgg ctgaagataa cttttttcat tgtatttgct tatacggcaa cgattgcgtc
     8281 atgcgaattg gcatgacgaa aacgttaaac taacgataaa ggaaagacat gtcccagata
     8341 gaagcggtat ttttcgactg cgacggtacg ctggtcgata gcgaggtcat ttgctctcgc
     8401 gcctatgtca cgatgttcag ggaatttggc attcacgttg acctgaaaga gatttttacc
     8461 cgttttaaag gcgtaaaact ctacgaaatc atcgatatca tcagtaaaga acagggcgtg
     8521 acgatggtcc aggccgatgc cgagcacgtt taccgcgcag aagtagcacg tctgtttgat
     8581 accgaacttg aggacatcgc aggagcgaac gaattgctgg cgagcattaa cgtgccgatg
     8641 tgcgtcgtct ctaacggtcc ggtcagcaaa atgcagcatt cgcttggtaa gctcaacatg
     8701 ctgcactact tcccggaaaa actgttcagc ggatatgaca ttcagcgttg gaagcccgat
     8761 ccggcattaa tgttccatgc ggcaaaagcg atgaatgtga acgttgaaaa ctgcattctg
     8821 gtcgatgact ccagcgctgg cgctcaatca gggatagatg ccggaatgga agtgttttac
     8881 ttctgcgctg acccgcacaa taagccgatc gttcacccga aagtcaccac ctttaccgag
     8941 ctgtcgcagc tgcctgcgct ctggaaggca cgcggctgga atataacgcg ataatcgcct
     9001 ccttacttca acacttacac atcagtcacg ctggtttgag atacagaaca ccaggtgtct
     9061 gatgagtaag cgtctattcc ctatgaataa tttatgtcgt tattcgtcct gtcatccacc
     9121 ctctgtatgg ttaagcctgg gttatatttt gtacaatatc gggataccgt tcgaaccaat
     9181 ccagaatatt acgcatcgcg tcactgatgt ttttatcgtc tttatattga ttctcaagtc
     9241 tattatttcg aacaatagta agcttagaac gtattttatc atcacaatcg acaattttcg
     9301 cattcaatgc atttacttca tcctgcactt ctatactatt ccaattgtcc cttttgttat
     9361 caaat
//
LOCUS       DAZKFZ010000181         9353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000181 DAZKFZ010000000
VERSION     DAZKFZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1100..3289
                     /gene="zorA1"
                     /locus_tag="ACHOQW_003753"
     CDS             1100..3289
                     /gene="zorA1"
                     /locus_tag="ACHOQW_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorA1"
                     /protein_id="HFP9574754.1"
                     /translation="MSWLNSILVTLTSVEPYKVPVTVIVTVTFAFVCFIFFYLLRSIR
                     IIYGLKKYTRSINSIEKSAPEVQLEHLKSLFQRSELKHAWNEFEESLHSQYELENGEE
                     KIVRIRATAPSASFFSEQQLVDIPLNTEFFKHLPGILTGMGIIGTFYGLMIGLNHFDP
                     STPEQVSSSVNNLLRDVLYAFLGSAFAIFASILVTWLEKLSIAKSYKYLEKFTAALDS
                     LYDSGVGEEYLASLVKSSNESATQARHLKESLVTDLRDMLLHLAESQKIENERLANTL
                     SATYRESGSQFADQVSGAIENSLKSPLDKIAGAVQTASGDQSGMVQNMLQNVLTAFMA
                     KLDTTFGQQFTNLNEMMGQTVGAIQTMQTGFSALLQDMRQVSDDSRQGSAQLIEQLLS
                     EMKSGQQALQAGMNDMLTSLQVSVAKIGAEGEGAGERIARQLEKMFADSEAREKAQAE
                     HMAAFVEAIQNSVQQGQSATMEKMAASVGALGEQLGSLFGQIDKGQQQISATQQANQQ
                     SLHEQTQRVMSEVDDQIKQLVETVASQHQGTTETLRLLAEQTNRQIQDMQAGADKMRL
                     AAERFEHAGERVSEANHLTADVLNKAQSAGSSLSLATSELTSVVADYRNNREAVSKSI
                     AMLELLAANTQSEQTTRNQFIADLKQHGERLQSYNREAQVFMENVSDVLGKGFEDFSE
                     GVSRSLDKTLGKLDVEMAKASNLLAGSVEQLGESVSELDDVLSRVRT"
     gene            3303..4043
                     /gene="zorB1"
                     /locus_tag="ACHOQW_003754"
     CDS             3303..4043
                     /gene="zorB1"
                     /locus_tag="ACHOQW_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorB1"
                     /protein_id="HFP9574755.1"
                     /translation="MFGNAFGVKKRRSDEAEKPFWISYADLMTAMMVLFLVVMVASLS
                     SVTQRIQRAEQGEKARGQDISRLCERLELHARNVNKNIVVDCHDNRISFGEAGRFAHN
                     QFFLNAEGQKALQDVVPLVLEASNSEEGKKWFKQIVIEGFTDTDGSYLYNLHLSLQRS
                     EWVMCSLLDSRSPLQKNISAEQQLQIRKLFLAGGVSFNNAKESKEASRRVELRMQFFG
                     LKDKRDKADEVDFPPVVNKEVCQLVMPL"
     gene            4040..5722
                     /gene="zorC"
                     /locus_tag="ACHOQW_003755"
     CDS             4040..5722
                     /gene="zorC"
                     /locus_tag="ACHOQW_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorC"
                     /protein_id="HFP9574756.1"
                     /translation="MIPALNSLSQRIAARLSSSQRDDHYLHNDFHALTAATLDMEKRF
                     DRAERIPSPPQEMRLEALRRFRLAQELTEREWRMVFYGLADNDPLYPDQPILLEDDTF
                     FPRVNSAIKKRIETKTLKRRDWAALCSSYFAYQHQSPETNPHWCVLRGHIAQGYLVVK
                     AAIRREKSWMKTIEFYHDIFTPQAGGVISRQLLAGENNSLSSLEKIAQIPDSSWLWKR
                     IFTVLLAQLDTLDDPQFLSKISWLLGLAAQWVRFRDDIMTATLTRYYHSGYRDQSHPA
                     LKQAALEYWDNPQLKSQQNKWHQYVSESVAAMVRGWLAKQDLTHFFELLRGNGDVDQA
                     RLHYWLRFANQMGFTRIIMGSDAWQDRGSDFVKFREENKGRLSYLRGGRNFDNAMVMQ
                     INDYLFVEFSGTGNAMYAYQIGHAPFNPESRTLDINIHLKDQGRCALRLPHAPRAEGY
                     NKVRITGWMLKYDDELRKLGIRWMAEEPVRFVDKKVPPPVAMSDIKIINPLRDTAIQH
                     LVKCSSCIVSDNRHKGGILSVQLITPDDTVEKELLRLGFAPVAKEPHRYWIK"
     gene            5722..8964
                     /gene="zorD"
                     /locus_tag="ACHOQW_003756"
     CDS             5722..8964
                     /gene="zorD"
                     /locus_tag="ACHOQW_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I Zorya anti-phage system protein ZorD"
                     /protein_id="HFP9574757.1"
                     /translation="MLKRLLSKLTGNRQQIEHHLKNQYQVEENGLSFPLSLVDDSQLW
                     ALASWLEQLAEEDYLISLTDRWLLSWEALYRLLEDEEHASSLPLIGVPDILPLRASLS
                     SRGALSDSDFRVWIAEWATFPARKPIRFSRTGAILTHDNQQYLLSRENWALLQATEQL
                     SAQQIQTPGETTNQLGWAAIRKCAKLAAAKFDDYLEKTHVIKPTSLSLRLRKATVADT
                     AVIEIEPHFEDQPANWLGSFDKNLQVHDSYRIPGENGELSHVIIPPEVKEVLNSIHSI
                     PGRRVAGSEALSFVRNPYTFLGEDAASVIAPEEHEQALFDARIFFHHFRLIPQLNTEN
                     KITEVTLILEPVSPVPQPEITFVFSAPWELDKFIQQLGISVAAQMPAGSWQGYELELS
                     QFTEQQWHDCQALLTRWQQEIEGKEFSDVLDIAKYGDRVIGIGEFEKISSPWLTKAQS
                     ENWLPDGIDFSAFSIETLSGWQPENPLHFDELQEIITQAEAAGETYITTPWNDSQLPL
                     DAAKTFSKNWEKQQITANEYQGNVADKTARAVLKIEQNIEETAYIKQRRNSLLNARHA
                     EPEIPLSLKEHIRLKDHQREGVAWLQQLFLRSPEETAGCLLADDMGLGKTLQILSFLV
                     WFIEKFPQEPPNLIVAPVSLLDNWERELNNFFYTAGIPVLKLYGETIKAVKYPKQAIP
                     AHLQSKGIKNLLKPGWQGEAKIILTTYETLRDQEFSLARQPWSIMVCDEAQKIKNPAA
                     LITHAANAVQARFKVACTGTPVENTLVDLWSLFDFAQPGLLGALNEFGKQYVRPIENE
                     DGRDTERLESLRALIEPQTLRRTKEEVARDLPQKIEVESCKQLTLSGVQKQLYLSSVA
                     NWQQQQALREGMQQAGTGMLGLLHRLKLICAHPAIVNPEPRFRDNSPKLNWLLKILAE
                     IKHTSKDKVIIFTELRDLQRELQHAIHQNFGFRPVIINGDSSTKSQSQNSRQRLIDDF
                     QAQPGFGVIILSTVAVGFGVNVQKANHVIHFTRCWNPAKEDQATDRAYRIGQTKNVYV
                     YYPTVRDTEITTFEETLDDLLQRRRALARDMLCATPDLNCADFETILKGA"
     gene            complement(9124..9290)
                     /locus_tag="ACHOQW_003757"
                     /pseudo
     CDS             complement(9124..9290)
                     /locus_tag="ACHOQW_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         2672 a         2202 c         2195 g         2284 t
ORIGIN      
        1 gcagatttat cgatcgatat aacacaaatg aacagttctt tattctgcta tatatcgcaa
       61 aaacagcaca ttgcaaaatt tataaccata attaatggaa aagcgcagaa aattattcca
      121 ctgaatgctt ttaatacagt tggttagctt aaaaaatagc aacttttgat ttcaatcatt
      181 gaaaattgca atgcgtgacg attcaacagc atttaagtga gtttcaggat atgggaagga
      241 catgaaagga ggagcgtaat aaaacacacc agaagaagga ggagaaaatg cttccctgta
      301 tatttatttt gtcaggccat ggcctttctc cagcaaccga tcaacgccac atacctgcag
      361 cgacactcaa cagaagtgag atcccatgca atatcatcat cacagggcca gcggaaggtc
      421 ccttgtacgg gcggtaggta cacagccata tgccatgttt atcctggcac aggactttta
      481 tgcaagtata ggctttgttg atgcagaacc atcataccat gcaaagctaa gttcgttctg
      541 gacatactgt atcaggatgc caatccccga cttccatcga caggtttacg atcaggaata
      601 cattatcaac cgtcagtatt gcgaaactct cagaagacat ctgaaaacat atttcagaag
      661 agaatccctg ttctacatac ttttagtcgg tagcctcatg caggctcgca ctttaccggc
      721 agcattccat aatttgtgat cctactcatt aagaaatcgt tatcaggtat tgttcatgaa
      781 aatcctcagg atggatcaca acggaagagt aatcatatct tatcgcagat tcagatttag
      841 aatgcggcac gtaaaatatt gtccagggga cgctgaccac gacacaacat caagaaattt
      901 gcttttgcat ggtaaaaaag aggaaactaa gaaaatgtct cacctgatta ttctgtttct
      961 atcgttcatt tattgaacag taagagtatt caggtgtaac cgcacgacat agtgacaacc
     1021 aatgcaacat aatcaattat gttgactatg ttgctgatga ctaattttag tatggataat
     1081 atattaaacg agtaaaacaa tgtcctggct taattccatt ttagtaacac ttacatctgt
     1141 agaaccctat aaggtacctg tcactgttat cgtcactgtt acctttgcct ttgtctgctt
     1201 tattttcttt tatttactga gatccatcag aattatttat ggattaaaaa aatacacgcg
     1261 ttcaattaac agcattgaaa aaagtgcgcc agaggttcag cttgaacacc ttaagagcct
     1321 gtttcagcgg tctgaactca agcatgcgtg gaatgagttc gaagaatcgt tgcactcaca
     1381 atatgaactg gagaacggcg aagagaaaat cgtccgtatt cgggctactg cgccgagcgc
     1441 cagcttcttc tcggagcaac aactggtcga tattccgctg aacactgaat ttttcaaaca
     1501 cctgcccggt attctgaccg gtatgggaat tatcggtacc ttctacggtc tgatgattgg
     1561 tctcaaccac ttcgacccca gcacaccaga gcaggtctcc agtagcgtca ataatctact
     1621 acgcgatgta ctgtacgcct tcctgggttc tgcatttgct atttttgctt ccattctggt
     1681 cacctggctt gaaaaattat ccatcgctaa aagctacaaa tatctggaaa aattcaccgc
     1741 agccctcgac tccctgtatg acagcggcgt tggcgaggag taccttgcct cactggtgaa
     1801 gtccagtaat gaaagcgcga cccaggcacg ccatctgaaa gagagcctgg ttacagattt
     1861 acgcgatatg ttactacatc tggccgaaag tcagaaaatc gaaaatgaac gcctcgcaaa
     1921 tacgttaagc gcaacttacc gcgaatcagg ttcacaattt gccgaccagg tcagcggtgc
     1981 gattgaaaac agcttgaagt caccattgga taaaattgcc ggtgcggttc agaccgccag
     2041 cggcgatcaa agcggcatgg tacaaaacat gctgcagaat gtactgaccg cgtttatggc
     2101 gaaactcgac accacctttg gtcagcagtt tacaaacctc aacgaaatga tggggcaaac
     2161 cgttggtgca attcagacca tgcaaaccgg tttctccgcg ttattacagg acatgcgtca
     2221 ggtgagcgac gattcccgcc agggcagcgc gcagcttatc gagcaactgt tatcagagat
     2281 gaaatctggc cagcaagctc tacaggctgg catgaatgat atgctcacca gccttcaagt
     2341 ctcagtggcg aaaattggcg cagaaggtga aggggccggt gaacgtatag ctcgccaact
     2401 ggaaaaaatg ttcgctgata gcgaggcgcg agaaaaagcc caggcagaac atatggctgc
     2461 ttttgttgaa gccattcaga attcagtaca acagggacaa agcgccacaa tggaaaaaat
     2521 ggcagcctct gtcggggcgc ttggtgaaca actgggtagc ctgtttggac agatcgataa
     2581 gggtcagcaa caaatttcag cgactcagca agcgaatcag cagtccctgc acgaacagac
     2641 tcaacgggta atgagtgagg tagacgatca gattaaacaa ttggtcgaaa ctgttgccag
     2701 ccagcatcag ggaacaacag aaactcttcg tttgctcgca gaacaaacta accggcagat
     2761 tcaggatatg caagctggcg cggacaagat gcgtctggct gctgaacgct ttgaacatgc
     2821 cggtgagcgg gtgtcggaag caaaccacct taccgctgat gtgctgaata aagcgcaatc
     2881 ggcaggttca tcactttccc ttgccaccag cgagctcacc tccgttgtgg ctgattatcg
     2941 taacaatcgg gaagcggtta gcaaatccat tgccatgctg gagttgctcg ccgcaaatac
     3001 gcagtcagaa caaaccacgc gcaaccaatt catcgcagat cttaagcaac acggtgagcg
     3061 tctacagagc tataaccggg aagcacaggt ctttatggaa aatgtaagtg acgtgctcgg
     3121 gaaaggattt gaagacttct ctgaaggtgt ttcacgcagc ctggataaaa cgctgggtaa
     3181 actggacgtc gaaatggcga aagcctctaa tctgctcgcg ggatctgtcg aacagctcgg
     3241 agaaagcgtc agcgaacttg atgatgttct gtcacgcgta cgaacctaag gggtgatggc
     3301 tgatgtttgg aaacgcattt ggcgttaaaa aacgccgcag cgatgaagca gaaaaaccat
     3361 tctggatctc ctatgccgat ctgatgactg caatgatggt gttatttctg gtcgtcatgg
     3421 ttgcgtcact gagttccgtt acacagcgta ttcaacgtgc ggagcaaggc gaaaaagcaa
     3481 gggggcagga tatttccaga ttgtgtgaac gcctggaact gcatgcccgc aatgtgaaca
     3541 aaaacatcgt agtggattgc cacgataacc gtatcagctt tggcgaagcc gggcgtttcg
     3601 ctcataacca atttttcctg aacgccgagg ggcaaaaagc gttgcaggat gtagttcctc
     3661 ttgtactgga agcgtccaac agtgaagagg gtaagaagtg gtttaagcag atcgttattg
     3721 aagggtttac cgacaccgac ggttcgtact tatacaacct acatctctcc ctgcaacgct
     3781 ctgaatgggt aatgtgtagt ctgctcgata gccgcagccc tctgcaaaaa aatatctccg
     3841 cagaacagca gctccaaatc cgtaagcttt tcctcgcggg tggcgtctcc ttcaataatg
     3901 ctaaggaaag caaggaagcc agccgccggg tcgagttgcg catgcagttc ttcgggctta
     3961 aagataaacg tgataaggcc gatgaggtgg attttccccc cgtcgttaat aaagaagttt
     4021 gccagttggt tatgccacta tgatacccgc cctgaattct ctttctcaac gcattgcagc
     4081 gaggctctct tccagccaaa gggatgacca ttatctgcat aatgatttcc acgcgttgac
     4141 cgcggctact cttgacatgg aaaagcggtt tgatagggca gaaagaatcc cctcaccgcc
     4201 acaagaaatg cggctggagg ctctacgtcg ctttcgcctg gcacaagaac tcacggaacg
     4261 agagtggcgg atggtgtttt atggcctcgc cgataatgat ccattatatc ctgaccagcc
     4321 gatcctgctg gaggatgata catttttccc cagagtaaac agtgcaatca aaaaacgtat
     4381 tgaaaccaaa acgttgaaac gccgggattg ggcagcactt tgttcaagct attttgccta
     4441 ccaacaccaa tcacccgaga caaaccctca ctggtgcgta ctgcgggggc atatcgctca
     4501 gggttacctg gtagtgaaag ccgctatacg acgagaaaaa tcgtggatga aaaccattga
     4561 gttttatcac gacattttta ccccacaagc gggaggagtg atctccaggc agttacttgc
     4621 aggtgagaac aattcactct catcattaga gaaaatcgcg caaatccctg atagcagttg
     4681 gttatggaag cgcattttca ctgtgctttt agctcaactg gacacgctcg acgatcccca
     4741 gtttctgagc aaaattagct ggttgttagg cttagctgcg cagtgggtgc gtttccgtga
     4801 tgacatcatg actgcaaccc tgactcgcta ttaccattct ggctatcgcg atcagtcaca
     4861 tccagcgttg aagcaggccg cgctggaata ctgggataac ccccagttga aaagccagca
     4921 aaataaatgg catcagtatg tctccgagtc ggttgccgca atggtccggg gctggttggc
     4981 aaaacaggac ctgacacatt tctttgaact gctgcgcggt aatggcgatg tcgatcaggc
     5041 tcgactgcac tactggctgc gtttcgctaa tcagatggga tttactcgta tcatcatggg
     5101 gtccgatgcc tggcaagatc gtggtagcga ttttgtgaaa ttccgcgagg agaataaggg
     5161 acgattgagt tacttacgag gtgggcgtaa ctttgataac gccatggtta tgcaaatcaa
     5221 cgattatctg tttgtcgagt tttccgggac aggaaatgcg atgtatgcct atcaaatagg
     5281 tcacgcgcca tttaaccctg aatcacgcac gcttgatatc aacattcatc taaaagatca
     5341 ggggcgatgc gcactcagac tcccccatgc acctcgtgct gaggggtata acaaagtccg
     5401 gataaccggc tggatgttga aatacgacga tgagctacgt aagttgggca ttcggtggat
     5461 ggctgaagaa cccgtcaggt ttgtcgataa gaaagtgccc cctcccgtcg ctatgtctga
     5521 catcaaaatc atcaacccgt tgcgtgatac ggctatacaa cacctggtta aatgtagttc
     5581 atgtatcgtc agcgataatc gacataaagg tggcatactg tccgtacaac tcattacacc
     5641 tgacgacacc gtagaaaaag agttgttacg actaggcttt gcacctgtag ctaaagaacc
     5701 gcatcgttac tggatcaaat aatgctgaaa cgtctgttaa gtaaactgac gggaaaccgt
     5761 cagcagatag aacaccacct aaagaatcag tatcaggttg aggaaaacgg tctcagcttt
     5821 ccgctatcgc tggttgatga ttcacagctt tgggcactgg catcctggct tgaacaactt
     5881 gctgaagaag actatctgat atcacttaca gatcggtggt tattgagttg ggaggcacta
     5941 tatcgcctgc tggaggatga ggaacatgcc agcagcctgc ctcttatcgg tgtacctgac
     6001 atattgccct tacgcgcaag cctgagttca cgaggagcat taagcgatag cgattttcgc
     6061 gtctggattg ctgaatgggc tacatttcca gcccgcaagc cgattcgttt cagtcgtacc
     6121 ggtgccattt taacgcatga taatcagcaa tacctgttat cgcgggaaaa ctgggcgctc
     6181 ttacaggcaa cagaacaact cagtgcacag caaattcaga ctcccggcga aaccactaac
     6241 cagctgggat gggccgctat ccgcaaatgt gcgaaactcg ccgccgccaa atttgatgat
     6301 tatttagaaa aaacgcacgt catcaaaccg acttcattgt cattacgtct gcgtaaagca
     6361 acagttgccg ataccgcagt tatcgaaatt gaacctcatt tcgaggacca gcctgctaac
     6421 tggctgggca gttttgacaa gaacttacag gttcacgata gctaccgcat cccaggagag
     6481 aatggcgaac tcagtcacgt catcatccct cccgaagtga aagaagtcct gaattcaata
     6541 cactcaatcc cagggcgtcg agtggccgga agtgaagcgc tttcctttgt tcgcaatcct
     6601 tataccttcc tgggtgagga tgccgccagt gttattgccc ctgaagaaca tgaacaggca
     6661 ctgtttgatg ccaggatttt ctttcatcat ttcaggttga taccgcaact gaatacggaa
     6721 aataagatca cagaagttac gctgatactt gagcctgttt cacctgttcc ccagccagaa
     6781 atcacttttg tgttctcagc gccctgggag ctggataaat tcatacagca actcggtatt
     6841 agcgtcgctg cacagatgcc tgccggttca tggcaaggtt acgagctgga attaagtcag
     6901 ttcaccgaac agcagtggca cgattgccag gcactgctaa ctcgctggca acaagaaatt
     6961 gaaggaaaag aatttagcga tgtgctggat attgcgaagt atggagatag agtgattggc
     7021 attggtgaat ttgaaaaaat atcctctccc tggctgacca aagcgcagag tgaaaactgg
     7081 cttcctgatg gcatcgattt ctccgcgttt tcaatcgaaa cactatctgg ctggcaacct
     7141 gaaaatcctc tccattttga tgaactacag gagataatta ctcaggcaga agccgctggt
     7201 gaaacgtata tcaccacacc atggaatgac agccaattac cgttggatgc cgctaaaacc
     7261 ttcagtaaga actgggaaaa acagcaaatc actgctaatg aatatcaagg caacgttgca
     7321 gataaaacgg cccgagccgt actcaaaatt gagcagaata ttgaagaaac ggcctacatc
     7381 aaacagcgcc gcaattcact cctcaatgcg cgtcatgccg aaccagaaat tcctctgagc
     7441 cttaaagagc atatccgact taaagaccat caacgtgaag gcgtagcctg gctccagcaa
     7501 ctcttccttc gttcacctga ggaaacagca ggctgcctgc tggcggatga tatggggctt
     7561 ggaaaaacgc tgcaaattct gagcttcctg gtatggttca ttgaaaaatt cccgcaagaa
     7621 ccgccaaatc tcattgttgc tcctgtctct cttttagata actgggaacg tgagctgaac
     7681 aatttcttct ataccgcagg aataccagta ttgaaattgt atggcgagac cattaaagcg
     7741 gtgaaatatc ccaaacaggc catccctgct catctgcagt ctaaggggat caaaaacctg
     7801 cttaaacctg gctggcaagg tgaggcaaaa atcatcctga cgacctacga aacgcttcgc
     7861 gaccaggagt tttctctggc acgccagcca tggtccatta tggtgtgcga cgaggcacaa
     7921 aaaataaaaa acccagctgc gttaatcaca catgcggcca acgcggtaca agccaggttt
     7981 aaagtggcat gcacaggaac gcctgtcgaa aacacacttg tcgatctgtg gagcttattt
     8041 gattttgccc agcctggatt gttaggggca ctaaatgaat ttggtaaaca gtatgttcgc
     8101 cccatcgaga acgaagatgg ccgcgataca gaacggctgg aaagcctgcg ggcgctgata
     8161 gaacctcaga ctttacgccg aaccaaagaa gaggttgcac gtgatttacc tcagaaaatt
     8221 gaagtagaaa gctgcaagca actgacgttg tctggcgtgc aaaaacagct ctacctttcg
     8281 tctgttgcaa attggcaaca acagcaagca ctgcgcgaag ggatgcagca ggccggaacc
     8341 ggtatgttag gtttgttaca ccgactgaaa ctcatctgtg cacaccctgc aatagttaat
     8401 ccagagccac gttttcgtga taactcaccc aagcttaact ggctgctgaa aatactcgca
     8461 gaaataaaac atacctcgaa agataaggtc attattttta cggagcttcg agatttacag
     8521 cgcgagcttc aacatgcaat ccaccaaaat tttggtttcc gccctgtcat tatcaatggt
     8581 gatagcagca ctaaaagtca aagccaaaac agccgccaac gcctgattga tgattttcag
     8641 gctcagcctg ggtttggggt gattattctc tctacggttg ctgtcgggtt tggcgttaac
     8701 gtccagaaag ccaatcatgt tattcatttt actcgctgct ggaatccagc caaagaagat
     8761 caggctacgg atcgggcgta tcgcattggg caaactaaaa atgtgtatgt ctattaccca
     8821 actgtgagag atactgaaat cacaacattt gaagaaacac tggatgatct cttacaacgc
     8881 cgacgagcat tggccagaga tatgctctgc gctactcccg atttgaactg tgcggatttt
     8941 gagaccattt tgaagggagc ctaaccgata tgagcggcaa tactatcagt tgccaaacct
     9001 gaaagaacac ttagctgtga tgattcgcta ttacctaccg atagcttcgg aaaaacataa
     9061 attaaaaatg cccagaggag tactattatc gttagtatta ttatccgaac gctgtattaa
     9121 cggttattta ttggtgagta ctacgttcca tggcaagagt tcatcaacag gttggaagac
     9181 cattccggca aaactctcag tatatggcgc agataccatt cagacagcag gtcccgctca
     9241 gcccgtacag tagagcgccg ttcactaccg taatcgctac cgaagaacac attgattgcc
     9301 cctcaataat cctgctgtta acatcaaaat tgttggtcgg gttcgattta ttt
//
LOCUS       DAZKFZ010000182         9310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000182 DAZKFZ010000000
VERSION     DAZKFZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            287..649
                     /locus_tag="ACHOQW_003758"
     CDS             287..649
                     /locus_tag="ACHOQW_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574758.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(828..1286)
                     /gene="ybbJ"
                     /locus_tag="ACHOQW_003759"
     CDS             complement(828..1286)
                     /gene="ybbJ"
                     /locus_tag="ACHOQW_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9574759.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1283..2200)
                     /gene="qmcA"
                     /locus_tag="ACHOQW_003760"
     CDS             complement(1283..2200)
                     /gene="qmcA"
                     /locus_tag="ACHOQW_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9574760.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2346..3023
                     /gene="fetA"
                     /locus_tag="ACHOQW_003761"
     CDS             2346..3023
                     /gene="fetA"
                     /locus_tag="ACHOQW_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP9574761.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3010..3789
                     /gene="fetB"
                     /locus_tag="ACHOQW_003762"
     CDS             3010..3789
                     /gene="fetB"
                     /locus_tag="ACHOQW_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP9574762.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3852..4706)
                     /gene="cnoX"
                     /locus_tag="ACHOQW_003763"
     CDS             complement(3852..4706)
                     /gene="cnoX"
                     /locus_tag="ACHOQW_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP9574763.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4767..5576)
                     /gene="ybbO"
                     /locus_tag="ACHOQW_003764"
     CDS             complement(4767..5576)
                     /gene="ybbO"
                     /locus_tag="ACHOQW_003764"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9574764.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5566..6189)
                     /gene="tesA"
                     /locus_tag="ACHOQW_003765"
     CDS             complement(5566..6189)
                     /gene="tesA"
                     /locus_tag="ACHOQW_003765"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP9574765.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6160..6846
                     /gene="ybbA"
                     /locus_tag="ACHOQW_003766"
     CDS             6160..6846
                     /gene="ybbA"
                     /locus_tag="ACHOQW_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP9574766.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6843..9257
                     /gene="ybbP"
                     /locus_tag="ACHOQW_003767"
     CDS             6843..9257
                     /gene="ybbP"
                     /locus_tag="ACHOQW_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP9574767.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2139 a         2386 c         2554 g         2231 t
ORIGIN      
        1 tgcaatttaa tcgaaatcta cgccgccatt tgtgtgatgt aaagcgcatt tttgtctgtt
       61 gtaagcgtaa acaccatcaa taagaaacaa aaatggtttc ttatataaat aaaaaaggaa
      121 atcatttcgt gctctttcaa tgtaaaaaag agtaaagact tgttttgata agcgaaataa
      181 attctgtttc tggactaaac gtgaagcggt tcctgatgtg tgctaaaatg cgtttctttg
      241 ataaatctca atttttaaga ggtttacgcc aggcaggaaa atttaaatga atgaatttga
      301 gaagattttt aatgaaatga atctggaccg ggcattgcta ccgatccttt ttcgttcgaa
      361 ccggtcgaca gtgtggaagt atttaagcgg cgatagtacg gctcctgcct ctgccatgtc
      421 gctgattatg ctgttgcagt tgatacagaa acgaaacccc gatctgttag ccgaatggtt
      481 aacgctttct gatttcacca ttccgccaga agtttacctg gaccagcctg attactggaa
      541 agggtgggtg tatacgcaac acaaggtcaa taaaaacgtc ctggaatatc taaaaaaaca
      601 ctatccggat gaagaccaaa aaagtatgag taaaggccgg gaagagtaaa ttgccaatgc
      661 cagttaacat ttaacatggc ggaggcggcc tacggttttg gcgtgatatg acgcgcccgc
      721 gtcgcatcag gcgtcggtgc acgactgccg gatgcggcgt aaacgcctta tccggcctac
      781 gattcgggcg cagacttccg ggcatgataa gacgcgtcag cgtcgcatca ggcgatgacc
      841 gcacggatga tcagcgttat cccttctatc gcaatgactt caacatgcgt acctgcgccg
      901 agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg accgcgcccg
      961 ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc gcgctggttt
     1021 aaatgactgt cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt ccaccacagc
     1081 caggcggcga gcagcgtcag gacggcaaac atcacccctt gccactccca acccagcggc
     1141 accagccaga ccaccaggcc agtaatcact gctgccacgc cactccacaa caaataacca
     1201 tttccgccca gcatctcggc tgccagcagc aaaccgccga gactgagcca gaaaatatgt
     1261 ggatgaacga ctattaactc catcatggct gagtccgctt gttggcgctg tctttcacca
     1321 gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc atcatcacta
     1381 ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttc tgcgctacga
     1441 agtagttcac cgcctgaata tcaccggagg cgatggcttc agacaccatt ttggtggcgc
     1501 gggcttctgc ttcagcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt
     1561 cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct
     1621 gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct ttcatctgcg
     1681 cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga
     1741 ctttaatccc ccacgggttg gtggcctcat cgacaatacg cagcaggcgt gagttgatgc
     1801 tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta cggatgttag
     1861 tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataagcc gcgcgtggcg
     1921 cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta tctttcgaga
     1981 taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc
     2041 gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa
     2101 aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga
     2161 ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta
     2221 ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga
     2281 gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa
     2341 cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat ctggcgggtg
     2401 atgcgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta
     2461 ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc
     2521 caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaag ccagaaatct
     2581 accgtcagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat acggtatacg
     2641 ataatctgat ctttccctgg cagatccgta accagcagcc tgacccggcc atttttctcg
     2701 attttcttga acgcttcgcc ttgccggaca gcattttgac gaagaatatc gccgagctat
     2761 ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg ccgaaggttt
     2821 tattgctgga tgaaatcacc agtgcgctgg atgaaagtaa taaacataac gttaatgaga
     2881 tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag
     2941 acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg
     3001 aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc attaatgctg
     3061 gtagtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa agatattctc
     3121 tggagcgtcg ggcgcgcgat aattcagctg attattgttg gctatgtgct gaagtatatt
     3181 ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg ctttaatgcg
     3241 gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc atcgtttatt
     3301 gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg gtcgattgaa
     3361 tttatcccga tgcaggtgat ccctatcgcc gggatgattg ccggtaacgc catggtagcg
     3421 gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca acagattcag
     3481 gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat tcgcgacagt
     3541 attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt agtgagttta
     3601 ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc gattaaatat
     3661 cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat aattgcctgc
     3721 tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac gcaattgaag
     3781 aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt catgtgccga
     3841 tcaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga cgccagtgca
     3901 tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg accgtctgcg
     3961 gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc attgcgccca
     4021 acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg attctccgcc
     4081 acctgctgtt gcaactgttg aatttccggc gtatcagccg cctgcttcag cagttcgatt
     4141 tgcgccacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt tttcagcacc
     4201 gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag caggccgatc
     4261 tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc atcggtgtaa
     4321 ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc gcgcggcagc
     4381 actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa gccatctacc
     4441 ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca
     4501 atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt gtactgcgcc
     4561 gcaaggcttt ccagaattgg ggttaactgc aaacagtgct ggctacgttc agaccaaaaa
     4621 tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt agattcgtta
     4681 atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat tttctttgac
     4741 atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat cacgcgcccc
     4801 ggcagcaggc gcttaagcac cattaccgcc caggtcacca gcgttaccgg atagcgcatc
     4861 ttcggcttct cgctaataaa agcatggcgt actttgtcca ccaccgcttc cggtcccaac
     4921 gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt ctggttgacg
     4981 ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt aattccgctg
     5041 cggcgcagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt actggccgcg
     5101 taagcgccac gacccggtgt ggagattaat cccatcaccg atgaggtcat cacaatacgc
     5161 ccttcaccgt gcggcaacat cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa
     5221 aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag ggggccatac
     5281 atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag ggcgatcacc
     5341 tcgtcggctg cgcgatcaac actttctggt gaatccagat cgatcaacac gccggtaaat
     5401 cccatgctgt tcatgcgctc aacatcatcc ggtttccggc aacctgccag cacatgaaaa
     5461 ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca tccggtaatt
     5521 aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg agtcatgatt
     5581 tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg cgtcgcggtt
     5641 gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt ccattaaaaa
     5701 gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc taaaggcttc
     5761 attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg gttcagcgtt
     5821 ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct gtggctgaaa
     5881 accacgcaaa ccgtcattgc cgcccagttc aaccagcacc caacgcggct gatgctgttt
     5941 cagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga tgctggcatt
     6001 gactaccgac gttttactct gccacttatc attcaacaag gcaggccagg ccgcgctggc
     6061 agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt ccgctgcggc
     6121 agcacggaag gttaacagga ccagaaacag gaagggcaaa tgccagcgga aaacattgtt
     6181 gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc catcctcacc
     6241 ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga gtcgggatcg
     6301 ggtaagtcaa ccttgctggc gatcctcgcc gggctggatg acggcagcag tggcgaagtg
     6361 agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg
     6421 aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa cgcgctggaa
     6481 aacgtcgagc ttccggcgct gctgcgcggt gagagtagcg cggaaagtcg taacggagcg
     6541 aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc ggcacagctt
     6601 tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaacggtcg acctgatgtg
     6661 ctgtttgccg acgaacccac cggtaacctt gaccgccaga cgggcgataa aattgccgac
     6721 ctgctgtttt ccctcaaccg tgagcatggc accacgttga ttatggtgac ccacgacctg
     6781 caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt gcaggaggaa
     6841 gcatgattgc acgctggttc tggcgcgaat ggcgctcgcc gtcgctatta attgtctggc
     6901 tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg
     6961 agaagggctt aagccaacaa agccgggagt ttatggcggg cgatcgggcg ttgcgcagtt
     7021 cgcgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca
     7081 agcagctgac tttcgccacc atgacatttg caggcgacac accgcagctg gcgaacgtta
     7141 aagcggtgga tgatatctac ccgatgtatg gcgatctgca aaccaatccc cctggcctga
     7201 aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt aacctgaaaa
     7261 cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa gtgattcagg
     7321 aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg aatctggcgg
     7381 atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgc tataaattcg
     7441 gcggcagcga gaaccagctc gacagctatg agaaatggtt gttacctcag cttaaacccg
     7501 aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg atggaacgct
     7561 cgcaacagtt cctgctgctt tcggcacttc tgaccttgct gctggcagtg gcagcagtgg
     7621 cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc ctcaaaacgc
     7681 tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg atggtgctga
     7741 cgctttcagc cgttaccggt ggggcgatag gcctgttgtt cgaaaacgtg ttgatggtgc
     7801 tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggcca tggctatggg
     7861 cgctgggcac catgacggtc atctcgctgc tggtggggct acgaccatac cgcttgttgc
     7921 tggcaacgca gcctttacgc gtattacgta atgatgtggt tgcgaacgtc tggccgctga
     7981 agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg
     8041 gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg
     8101 tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg ctgcctctgc
     8161 gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag ctttcggcat
     8221 tttcgctctc ctttatgctg ctggcactgc tactggtgct gcgtggcgat ctgctcgacc
     8281 gctggcagca gcagttaccg ccagaaagcc cgaactactt tttaatcaac atcgccacag
     8341 aacaggttac gccgctaaaa gcgttcctcg cggaacatca gatagtcccg gaatcgtttt
     8401 atccggtggt gcgagcgcgg ctgacggcga ttaacggcaa gccgacagaa ggcaatgaag
     8461 atgaggcgct caaccgcgaa ctcaatctta cctggcaaaa tacgcggccc gatcataatc
     8521 cgattgtcgc cggtaactgg ccgccaaaag ctgatgaagt ttcgatggaa gaggggctgg
     8581 cgaaacgctt aaacgttacc ctcggcgata ccgtgacttt tatgggcgat acccaggagt
     8641 tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg cctaatttct
     8701 attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt accagtttcc
     8761 gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttccct accattagcc
     8821 tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag gtaagtcggg
     8881 cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg ctggcacagg
     8941 tgcaggtggg aatgcgtcag cgtcatcagg agctggtggt gtggcgcaca ctcggtgcgg
     9001 ggaaaaaact gctgcgtacc acgttgtggt gtgagttcgc catgcttggg tttgtttccg
     9061 gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg aaagtgtttg
     9121 attttccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc ggtgcgctgc
     9181 tgctgtcgct tttcggcggc tggctgggcg cgagactggt taagggtaag gcgctgttca
     9241 ggcagtttgc ggggtgatga gagtaaataa ggggcgttct ggttgaatgg aacgccttgt
     9301 ttaaattaat
//
LOCUS       DAZKFZ010000183         9203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000183 DAZKFZ010000000
VERSION     DAZKFZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(138..461)
                     /locus_tag="ACHOQW_003768"
     CDS             complement(138..461)
                     /locus_tag="ACHOQW_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP9574768.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(469..654)
                     /gene="ynbE"
                     /locus_tag="ACHOQW_003769"
     CDS             complement(469..654)
                     /gene="ynbE"
                     /locus_tag="ACHOQW_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP9574769.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(651..3290)
                     /gene="ydbH"
                     /locus_tag="ACHOQW_003770"
     CDS             complement(651..3290)
                     /gene="ydbH"
                     /locus_tag="ACHOQW_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP9574770.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            3498..4487
                     /gene="ldhA"
                     /locus_tag="ACHOQW_003771"
     CDS             3498..4487
                     /gene="ldhA"
                     /locus_tag="ACHOQW_003771"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9574771.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4598..5020
                     /gene="hslJ"
                     /locus_tag="ACHOQW_003772"
     CDS             4598..5020
                     /gene="hslJ"
                     /locus_tag="ACHOQW_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP9574772.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5017..5283)
                     /gene="ydbJ"
                     /locus_tag="ACHOQW_003773"
     CDS             complement(5017..5283)
                     /gene="ydbJ"
                     /locus_tag="ACHOQW_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP9574773.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5557..9081
                     /gene="nifJ"
                     /locus_tag="ACHOQW_003774"
     CDS             5557..9081
                     /gene="nifJ"
                     /locus_tag="ACHOQW_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9574774.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2288 a         2405 c         2265 g         2245 t
ORIGIN      
        1 ggtaatgatt tgttatttaa gggaaggata tataaggttt ttgtagaaat acccacggta
       61 attaagcatg ctaacgtcag tttaaatact gtaaaagatc attacattaa tagatacatt
      121 ttgtagccaa ctataattta tttgcgcaac catttaccat taactccctg aacatattgc
      181 cccgacttcg cgcgagcaac aagcttttgt ccagccagtt tggctatctc tttagtggat
      241 acattatttt gctttgccag ttgttggtaa ctatggttac gagcttcatt aatatctttt
      301 actaatgcct gtgtttcggc atccgtttgt agcgccacca ggtaaccgtt aagcgtttca
      361 cctacccgcc cctgacttct ggcttcgttc agggttaaag ccattgcact gtcgctcatc
      421 aggccaatgg cccatacagc tatgagtaat gttcttttca tcatcacctc aaaatagatc
      481 gctacgggtt ttgagcaggt cttcaacgtc cttatccgct ttaatgatga tttcatgctc
      541 aattttaacg ttcatattaa tggtgattgg ctctttcggc gcagccactt caatgcgagg
      601 cgtgcaacca accaacataa aagaggacgc aaacgcggct atcactattt tcattgtttt
      661 tcctcacact cttttcctga tgggcagcca gtttccggta gctgggcatt ctgctccagc
      721 catacctgga gattatcgcc aaagcgtaaa ctgcgccata acgtaaaaat attctcttca
      781 tggtgataat taaggtttac cgtaccgctt ttaccatcga cgcgactggt gcccgaaatg
      841 ttggcctgca tggttaacac accgagatta tctaaattaa tccttgttga tgagcgactg
      901 atttccatat aacgcaacca gttgatggca gttccagcgg tcatattgtc tttggccacc
      961 gcatctgcag tgtctttatc cagacgcagc gtcatcggcc ccgcgtttgc caaccagcca
     1021 tctttgacaa tccatttttc attgttcagc catagtggca acgccccact aaatgcccca
     1081 gacatagcaa actgtttcgg attaaccgcg ctaaccagtt cgctggatga gagattattc
     1141 agccgcaaca gcgccgggtc atgttgcggc atacgtaatt gtttcatcag cacgttaccg
     1201 cctaacacat cgacgctaac atcagtcaac agcaatggtt cttcttcagt ccacggataa
     1261 cgcccttgca aatcagccgt aatatttttc gctgtcacca gattaatcac ttcggcaatt
     1321 cgcaacgtaa cggggccgcg agtccccaga tgccaggctc catcggcaaa acggaaaggc
     1381 aggacaaaat cgacaccgtt aacctgatta tctggcatcc aggcactacc gcctttcaac
     1441 acgccgtgcc ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc aacctgagca
     1501 tacagttcac catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg ctggaataca
     1561 gtcagtgatt gtttaggcca ccaggcgtta ccgcgcagac gaataccgtc ccagcgacca
     1621 tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag gaaatacgtc
     1681 ggatcgcgcc catcgacgct aaattttaag gttgatggcg gtaacacact gccgccagtg
     1741 aacagcgttt gcccggcgtc cagtgacagc gcgccgctga aggagggatg ctgtgcgtca
     1801 cgtacccaga cgacgggctt gtcgagaatt aatcgcggct tttctaccgt catcgtaccg
     1861 tattgtaact ggtcgaaacc ggtggagaga tcggtcagcg taatcgtgct gtcatgccac
     1921 tcaccttttc ctgcgacatc ccaggtggca ttcatcggtg taaaactccc ttttccccag
     1981 tagcgccact gccagcggcc agcgtcaggg agaaaatcat tcgccagccc atccatatgc
     2041 agcacgaaat cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa acgtccgtca
     2101 acaccacgtt gggtgacttt tacacccgct aaaggccagc ggatttcatc gatgtccagc
     2161 gaatcgatga ctcttccctt tgaacgaagt aacgcgcctg gctcaaaggt cagcgttggg
     2221 tcagacagac ttccacttag ctgcgcaggt aaacgggcat ataaaatgag gtccgcctgt
     2281 ttcgcttcac cggtcagctg cagaggcagc tggctgttat ccatgcttaa ttttcctggg
     2341 ccaaaattaa gcaccgcgtt gcccttaccc gcttgcccct gggtcagcac actcagtcgt
     2401 ccgctgacca gagcgttctc aagccctgcc tgccagttgt cgactttgac acccagtcgg
     2461 ccactcaaag gaaaacctgc atacggccag ctccagcgac catcgcttac ggtcaattgt
     2521 tgacgagtaa tttgccacgg caaatcgagc aacggatcgc cgttatcccg tgccagcacg
     2581 atcaattgcc cgctattttc ctgccagtcc agctcggcat ccaccagtga cggttcctgc
     2641 ggcaagttta acgtcgcagt agcatgccca cttacaggaa gtccatccgg catgagcggc
     2701 atagtaaatt cccccaccag tttaaccgac ggctgatttt caaacgcgac gacatccagt
     2761 tcgctgactg taagttgttg ccctttcagc tggccttgaa atttaacttt ttcgccctga
     2821 taacgcagtt gctggatatc agaggttaat gcgagagaga gttttccctg ccattcctgc
     2881 cagggagaaa aaatcagttt atcaatattg atccaggtgt taggcagcat ggactgccat
     2941 tgcgcgaggg tttttggagc agctggcgat tgctccgtct gcggcaattt cgccagacaa
     3001 gcagaatcaa gttctaccgt gccgacgttc aataaccagc ggctgggatg tgaaaggctg
     3061 gcgttggtga tatgcgcaag ctgacaatct cccaccagat aacggagatc gggaatgatt
     3121 aaacctttac gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc gagcggtagc
     3181 cagatgcccg ccagcgtggg aacccacagc ccgagcgtca tcagcagcgt caacggcaca
     3241 agaataatca gtaataacag cgcgagaacg gctttatatt tacccagcat gggtagttaa
     3301 tatcctgatt tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg tttaaccgtt
     3361 cagttgaagg ttgcgcctac actaagcata gttgttgatg aatttttcaa tatcgccata
     3421 gctttcaatt aaatttgaaa ttttgtaaaa tatttttagt aacttaaatg tgattcaaca
     3481 tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt
     3541 acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga
     3601 cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc gtaaacgatg
     3661 acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccctgc
     3721 gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag
     3781 tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt atgatgatga
     3841 cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac ttctctctgg
     3901 aaggtctgac cggctttact atgtatggca aaacggcagg cgttatcggt accggtaaaa
     3961 tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc
     4021 cgtatccaag tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt
     4081 tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac taccatctgt
     4141 tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg
     4201 gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa attggttcgt
     4261 tgggtatgga cgtgtatgag aacgaacgcg atctgttctt tgaagataaa tccaacgacg
     4321 tgatccagga tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc
     4381 accaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg ctgcaaaact
     4441 taagcaatct ggaaaaaggt gaaacctgcc cgaacgaact ggtttaatct tgccgctccc
     4501 ctgcaatcca ggggagctga ttcagataat ccccaatgac ctttcattct ctattcttaa
     4561 aatagtcctg agtcagaaac tgtaattgag aaccacaatg aagaaagtag ccgcgtttgt
     4621 tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg ttacgccaga
     4681 acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgacctgcga
     4741 taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca gcatgtgtaa
     4801 ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag ggctggcaat
     4861 gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat
     4921 gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa
     4981 acagacatta acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc
     5041 ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt
     5101 accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc
     5161 actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac
     5221 aggctcacta ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg
     5281 catctcttct tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca
     5341 gagggcgacg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat
     5401 ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga
     5461 aaatgcagaa gaaagccatt tgctaaaatt gaaagattac tactgggcgc gcagcaattt
     5521 cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc
     5581 gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc tattaccccc
     5641 agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttgaa gaacgtttgg
     5701 ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat
     5761 ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg tttgctgctg
     5821 atgatcccga cgctgtacaa actggcaggc gaactcacac cgtttgtcct gcatgtagcg
     5881 gcacgtaccg ttgccacaca tgcactctct atttttggcg atcattccga cgtcatggcg
     5941 gtgcgccaga cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc gcaagacttt
     6001 gctctcattt cgcacatcgc gacgctgaaa agccgcgtgc catttattca tttctttgat
     6061 ggtttccgta cgtcccacga aatcaataaa attgtcccgc tggccgatga cactattctt
     6121 gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcgctcaa cccggaacat
     6181 cccgtgatcc gcggtacgtc agccaatcct gacacttatt tccagtctcg cgaagccacc
     6241 aacccatggt acaacgcggt ctatgaccat gttgaacagg cgatgaatga tttctctgcc
     6301 gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc ggaacgggtg
     6361 attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc
     6421 cgtggcgaaa aagttggcgt gctgaaagtt cgcctgtacc gtcccttctc cgccaaacac
     6481 ttgctgcaag ctctgccggg atccgtacgc agcgtggcgg tactggacag aaccaaagaa
     6541 cccggtgccc aggcagaacc gctctatctg gatgtaatga ccgcactggc agaagccttt
     6601 aataatggcg agcgcgaaac tctgccccgt gtcattggtg ggcgctatgg tctttcatcc
     6661 aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg
     6721 aaagcgcgct ttacggttgg tatttacgat gatgtgacca atctgtcact gccgttgccg
     6781 gaaaacaccc tgccaaactc ggcgaaactg gaagccttgt tttatggcct tggtagtgat
     6841 ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac
     6901 gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt ttctcacctt
     6961 cgcgtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc
     7021 tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa acctggcggc
     7081 attttcctgc tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaggaa
     7141 gttcaggccg tgctaaatca gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc
     7201 gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat
     7261 ctgacacaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa
     7321 agttacagta gcaaaggtca ggatctggtg gaacgcaact ggcaggctct ggcgctggcg
     7381 cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg
     7441 ccagtggttt ccgatgccgc ccctgctttc gtgaaaaccg taaccgctgc gatgctcgcc
     7501 gggcttggtg atgccctccc cgtttcggcg ctgccgccag acggcacctg gccgatgggc
     7561 accactcgct gggaaaaacg caatatcgcc gaagagatcc ccatctggaa agaggaactc
     7621 tgtacccaat gtaaccactg cgtcgccgct tgcccacact cagctattcg cgcaaaagtg
     7681 gtaccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg
     7741 cgtgatatgc gcgggcagaa atatgtcttg caggtggctc cggaagattg caccggttgt
     7801 aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc
     7861 aatatgatgt cgcgcctgga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc
     7921 aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgcac atcgcagctg
     7981 attacaccgc tgtttgaata ttccggtgct tgctccggtt gtggcgagac gccgtatatt
     8041 aaattgctga ctcagctcta tggtgaccgg atgttgatcg ctaacgccac cggctgttct
     8101 tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa cggtcgtggg
     8161 ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg tttccgcctg
     8221 acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc
     8281 ccggcggaat tactgacggc gttgaaatca gacgccacgc cagaggttcg tcgtgaacag
     8341 gttgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact gctgcgtgac
     8401 gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gcgacggctg ggcttacgat
     8461 atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg
     8521 ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac accgctgggt
     8581 gcagtaacta aatttggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt
     8641 atgatgatgt acggtcatgt ttatgtggct cagatttctc tcggcgcgca gctgaaccag
     8701 acggtgaaag cgattcagga agcggaagcg tatccgggac catcgctgat cattgcttat
     8761 agcccgtgtg aagagcatgg ttacgatctg gcactcagcc atgaccagat gcgccaactc
     8821 acagctaccg gcttctggcc gctatatcgc ttcgatccgc gtcgtgccga tgaaggcaaa
     8881 ctgccgctgg cgctggattc acgcccgccg tcagaagcac tggaagaaac gttacttcac
     8941 gagcaacgtt tccgtcgtct gaattcgcag cagccagaag tggcagaaca gttatggaaa
     9001 gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg
     9061 gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta aaaaaaagcc
     9121 cggacgactg ttcgggcttg tctttttata tgttggaaaa tcagtggcaa tgcaatggcc
     9181 caacagaaaa taaaatctgt gac
//
LOCUS       DAZKFZ010000184         9195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000184 DAZKFZ010000000
VERSION     DAZKFZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..269
                     /locus_tag="ACHOQW_003775"
     CDS             <1..269
                     /locus_tag="ACHOQW_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574775.1"
                     /translation="RNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYK
                     SRHEMLPVNLTPETLFGSKSYDKHALFPIFTASWRANRIKNKGI"
     gene            492..989
                     /gene="ubiC"
                     /locus_tag="ACHOQW_003776"
     CDS             492..989
                     /gene="ubiC"
                     /locus_tag="ACHOQW_003776"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP9574776.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1002..1874
                     /gene="ubiA"
                     /locus_tag="ACHOQW_003777"
     CDS             1002..1874
                     /gene="ubiA"
                     /locus_tag="ACHOQW_003777"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP9574777.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2029..4452)
                     /gene="plsB"
                     /locus_tag="ACHOQW_003778"
     CDS             complement(2029..4452)
                     /gene="plsB"
                     /locus_tag="ACHOQW_003778"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP9574778.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4623..4991
                     /gene="dgkA"
                     /locus_tag="ACHOQW_003779"
     CDS             4623..4991
                     /gene="dgkA"
                     /locus_tag="ACHOQW_003779"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9574779.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5101..5709
                     /gene="lexA"
                     /locus_tag="ACHOQW_003780"
     CDS             5101..5709
                     /gene="lexA"
                     /locus_tag="ACHOQW_003780"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9574780.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5782..7107
                     /gene="dinF"
                     /locus_tag="ACHOQW_003781"
     CDS             5782..7107
                     /gene="dinF"
                     /locus_tag="ACHOQW_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP9574781.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7223..7432
                     /gene="yjbJ"
                     /locus_tag="ACHOQW_003782"
     CDS             7223..7432
                     /gene="yjbJ"
                     /locus_tag="ACHOQW_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9574782.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7474..7989)
                     /gene="zur"
                     /locus_tag="ACHOQW_003783"
     CDS             complement(7474..7989)
                     /gene="zur"
                     /locus_tag="ACHOQW_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP9574783.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8308..>9195
                     /locus_tag="ACHOQW_003784"
     CDS             8308..>9195
                     /locus_tag="ACHOQW_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP9574784.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKGKVNSIYGKSIDYSTM
                     RHRDIIIAKIDLFIQRITHNLWTAREKNVTLIKQINDLKMCVNEYIGDCTDEELNDRE
                     FIASVVDRAIFHFAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEESKDNE
                     PLANIHLQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNINEYN
                     DNYLKAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKM
                     RDAAPKLIDFAKSFDPNSKE"
BASE COUNT         2202 a         2156 c         2438 g         2399 t
ORIGIN      
        1 cacgaaacga taatatcttt atcttgttct cctctgtcga tgatgggcca gttatggtgg
       61 taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca
      121 cgtatatcta taaatccaga catgagatgt tgcctgttaa tcttactccg gaaacacttt
      181 tcggctccaa atcttatgat aaacatgcgc ttttccccat ttttactgcg agttggcgag
      241 ctaatcgtat aaagaataaa ggtatttaag taaaggaaaa catcaccgct cttggcatca
      301 tgggcggtga tgcccctatg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt
      361 caacagcgtt ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa
      421 tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg
      481 gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattgtaaag
      541 agatccctgc cctggagccg caactgctcg actggctgtt gctggaagat tccatgacaa
      601 aacgttttga acagcaggga aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg
      661 agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac
      721 gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg
      781 tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac
      841 gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg
      901 gactgtgggg gcgacgttcc cgcttgcgat taagcggtaa accgctgttg ctaacagaac
      961 tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc
     1021 agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac
     1081 tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt ccccagctct
     1141 ggatcctggc ggtgtttgtc gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga
     1201 atgattatgc tgaccgcaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc
     1261 ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt
     1321 cgtttttact ggtgctgacg ctgaatacga tgaccattct gttgtcgatt gccgcgctag
     1381 cgctggcgtg ggtgtacccg tttatgaagc ggtataccca tctaccgcaa gtggtgctgg
     1441 gcgcggcgtt tggctggtcg attccaatgg cttttgctgc tgtgagtgag tcggtgccat
     1501 tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt
     1561 atgcgatggt tgaccgtgat gatgatgtga agattggcat taaatccacc gcaatcctgt
     1621 tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg gcgctgatgg
     1681 cgattatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt ctggtggctg
     1741 gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgcgaa gcctgcttta
     1801 aagcatttat gaataataac tatgttggtc tggtactgtt tttagggctg gcaatgagtt
     1861 actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc
     1921 tgatgcgctg cgcttctcag gcctacacaa cagatcgcaa tttattgaat ttgcagatta
     1981 tggaaggccg gataaggcgt ttgcgccgca tccggcaatt ctctctgatt acccttcgcc
     2041 ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg
     2101 ataaaccttc atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc
     2161 acgcagcgta agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat
     2221 gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg
     2281 gttgatcgac gggttggcgc tcaacagcca gaaggtgatg gcataacgtt gcagcgtttc
     2341 gcgcgcgcct gcggcgagca gctgtagcgt gcgagaatgc accgggttga tatgcaactc
     2401 atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac
     2461 gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag
     2521 cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc
     2581 cgccatcagc gaaggtagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag
     2641 caccgcttgc tcgcgcggca gaatgatgat gtcgccgata gtgtctttct caacttcaaa
     2701 cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga
     2761 gtccgtggag taaggcacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa
     2821 ctgctcgcgg gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat
     2881 ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac
     2941 cgtcggcgtt aaccatgccg gacgcactgc ttcgatggga tcgatagatt cacgccagtc
     3001 tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaaat tgacgtaacc
     3061 ctggccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc
     3121 tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc
     3181 gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt
     3241 catcgacagc gtaccggttt tcggatccag caaacgcccc gtacgggaac gaccgccttc
     3301 tacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac
     3361 ggtggaatag agcttattgc ctttaaaagt acggcgaata aagaacgcac ccagacggcg
     3421 gaaaatcggc ccggcaggcc agaaattcag gttgatcccg gcggcgatat gcggcggcac
     3481 tagcccctgg tggtacagca cgtaagaaag cagcaggtag tccatgtgac tgcggtggca
     3541 aggcacatat accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg
     3601 gacgttgatg ccctggtaaa ggcggttcca ggtaaagccc agaatacggt cagtcaggcg
     3661 gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg
     3721 cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc
     3781 gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg gtcctacggc
     3841 agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gcgcgatagt
     3901 tttatccgtg ccgtgttcat ccgccatacg gcgcagcgaa actgacggcg agaaacgcac
     3961 aaaactgtcg cgaccgagcc acagtacagc gaaaaatttc tgtacgccgt taagcatacg
     4021 cagcggcggg ttcacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgacac
     4081 tggcaccatc tgcacatcca gatttgggtt gctacggtgc aaatcgagat agtcgtggaa
     4141 cagcttaata gactcttctt tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat
     4201 gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa
     4261 gtcatgtgcc agacactggg cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa
     4321 aacgtacata attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat
     4381 agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg
     4441 ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata
     4501 gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac
     4561 ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt
     4621 caatggccaa taataccact ggattcaccc gaataatcaa agctgctggc tattcctgga
     4681 aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat
     4741 tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc gattacccgc gtgctgctta
     4801 tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg
     4861 ttgaccgaat tggctctgaa taccatgagc tttccggacg cgctaaagat atgggatccg
     4921 ccgcggtgct gattgcgatt atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc
     4981 attttggata acccttccag aattcgataa atctctggtt tattgtgcag tttatggttc
     5041 caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga
     5101 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     5161 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     5221 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgtaattga aattgtttcc
     5281 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc actggtaggt
     5341 cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc
     5401 gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     5461 aaagatatcg gcattatgga tggcgacttg ctggcagtgc ataaaactca ggatgtacgt
     5521 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa
     5581 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac
     5641 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gcgttattcg caacggcgac
     5701 tggctgtaac atatctctga gaccgcgatg ccgcttggcg tcgcggtttg tttttcatct
     5761 ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc actttggcat
     5821 ctcgccttac ccatgatttt ctccaatatc actgttccgt tgctgggact ggtcgatacg
     5881 gcggttattg gtcatcttga tagtccggtt tatttgggcg gcgtggcggt tggtgcaacg
     5941 gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact
     6001 gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaacctttg
     6061 ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc gtacgccgat tatcgatctg
     6121 gcgctgcata ttgttggcgg tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa
     6181 atccgctggt taagcgcacc ggcgtcgctg gcgaatctgg tattacttgg ttggttactc
     6241 ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt
     6301 gtgctggatg tctggctggt gatgggactg catatgaacg tgcggggcgc ggcgctggcg
     6361 acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc
     6421 aaactacgcg gaatctccgg cgaaatgctg aaaacttcct ggcgaggaaa cttccgtcgc
     6481 ttgctggcac ttaaccgcga tatcatgctg cgctcgctgt tgttgcaact ctgtttcggc
     6541 gcgatcaccg tacttggcgc gcgactgggg agtgacatta tcgctgttaa cgcggttctg
     6601 atgacgctac tcacctttac cgcctatgcg ctggacggtt ttgcctacgc ggttgaagca
     6661 cattccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca
     6721 gcatgccgcc agtcggggat cgtagcgtta ctgttttcgg tggtttattt gctggcaggg
     6781 gaacacatca ttgcgttact ggcgtcgtta acccagattc agcagctggc cgaccgctat
     6841 cttatctggc aggtgatttt gccgttggtc ggcgtctggt gttatctgct ggacggcatg
     6901 tttataggcg caacgcgtgc tgctgaaatg cgtaacagta tggcggtggc cgccgcaggt
     6961 tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gttggcgtta
     7021 accgtctttc tggcgttgcg cgggctttct ctggcagcta tctggcggcg tcactggcgc
     7081 aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata atcctaagcc
     7141 aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca
     7201 acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt
     7261 taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga
     7321 aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca
     7381 ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc
     7441 tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac
     7501 agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc
     7561 acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag
     7621 cgtatgcatg atgtcttcca cgccttcagc acattcttct ttcaccgcgc cgcagcgatc
     7681 gcaaataaac atagctgacg tatgggtggg ctggtcgaac agatggcaga gcacataact
     7741 gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata
     7801 aaccgttggc ggcttggctt gcggttcagc ttcgcgcagc aaatcaagca gatcataagc
     7861 gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag
     7921 gcgcacatta cgctgcgcgc agattttgtc agcctgcgct aataactcct gcgttgtggt
     7981 cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag
     8041 gaaaaacacc gagaaccctt attgttgccg taatattgct tttctgtttt gtaggtaaga
     8101 tgttgtgttg cctggcagta ccattatcaa cacgataata attaataatt catttttaaa
     8161 taaattgctt caagttaact cgctatttat ttaaccgtgg ctaatcgtat acccgaattt
     8221 cagaaaatta tgagcgattt ttgatagtta ccggttccaa aaacacttat aaggaggaca
     8281 cggtacgaca aaaaaggtag atgaatcatg ttaaagataa tacccggtgc aacaggatac
     8341 tttaataaaa cactcaattc aaatcaattc gataatgaag atgcaatcaa aggtaaagta
     8401 aatagtatat atggtaaatc tattgattat tcaacgatgc gtcatcggga tataattatt
     8461 gcaaaaatag atttatttat tcaaagaatc actcataatt tatggactgc acgtgaaaaa
     8521 aatgtgacgt taattaagca aataaacgat ctcaaaatgt gtgtcaatga gtatattgga
     8581 gattgtactg atgaggaatt aaacgatcgt gaatttattg catcagttgt tgacagggct
     8641 atttttcatt tcgcgattaa tagtatatgt aatcccgagg ataataaaga tgcgacactc
     8701 atagaacgat gtacttttga tgtagaaacc aagaatggtc ttccctccac ggttcagcta
     8761 ttttatgagg aatctaaaga taatgaacct ttagcgaata tacatttgca agcaataggt
     8821 tctggtttct taacgtttgt taatgcctgc caggaacatg atgacaacag cttaaaatta
     8881 tttgcttcgc tgttaatttc cctttcatat tctagtgtct actcagattt tgcaggaaga
     8941 gtgaatatta atgaatataa tgataactac ctgaaagctc agtttgaaga attatctcaa
     9001 cgtgatatga agaagtacct gggagagatg aagcgtctgg cagatggggg agaaatgaat
     9061 tttgatggct atctggataa gatgtcacat ctggtgaatg aaggaacgct cgcccctgat
     9121 attttaagca aaatgcgaga tgctgcaccg aaattaattg actttgctaa gtcgtttgac
     9181 ccaaactcaa aggaa
//
LOCUS       DAZKFZ010000185         9180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000185 DAZKFZ010000000
VERSION     DAZKFZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..253
                     /locus_tag="ACHOQW_003785"
     CDS             <1..253
                     /locus_tag="ACHOQW_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9574785.1"
                     /translation="KNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDL
                     TPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            225..2154
                     /locus_tag="ACHOQW_003786"
                     /pseudo
     CDS             225..2154
                     /locus_tag="ACHOQW_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2138..2344
                     /locus_tag="ACHOQW_003787"
     CDS             2138..2344
                     /locus_tag="ACHOQW_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9574786.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2341..3933
                     /locus_tag="ACHOQW_003788"
     CDS             2341..3933
                     /locus_tag="ACHOQW_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9574787.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3923..5428
                     /locus_tag="ACHOQW_003789"
     CDS             3923..5428
                     /locus_tag="ACHOQW_003789"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9574788.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5465..5812
                     /locus_tag="ACHOQW_003790"
     CDS             5465..5812
                     /locus_tag="ACHOQW_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9574789.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5870..6121
                     /locus_tag="ACHOQW_003791"
                     /pseudo
     CDS             5870..6121
                     /locus_tag="ACHOQW_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            6118..6858
                     /locus_tag="ACHOQW_003792"
     CDS             6118..6858
                     /locus_tag="ACHOQW_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9574790.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6872..7303
                     /locus_tag="ACHOQW_003793"
     CDS             6872..7303
                     /locus_tag="ACHOQW_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9574791.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            7330..7743
                     /locus_tag="ACHOQW_003794"
     CDS             7330..7743
                     /locus_tag="ACHOQW_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9574792.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7724..>9180
                     /locus_tag="ACHOQW_003795"
     CDS             7724..>9180
                     /locus_tag="ACHOQW_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9574793.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALET"
BASE COUNT         2105 a         2244 c         2901 g         1930 t
ORIGIN      
        1 aaaaaatgag gtgtcaaaag gaaaactgat tgacaccggg ttctgtattt ttgccctcag
       61 taagctggca atggcgttat ccagtacgct tgattccatc cctttatcca tgcagcgaca
      121 gtttcctgat ttaacaccgc gccatcttga ccatctgaaa acccttattg ctaagggggc
      181 aaatcagtgt gcgcgggcag gggataaatt accggattta cttgatgaat atatcagagc
      241 aacaactgaa taatatgatg agcgctgtca caactgcatt acagcccctg ataagggcat
      301 tgccggtgac gccagttgaa tgggctgatc aaaattatta tctgcctaaa gaatcttcat
      361 atggtgaggg cgaatggaaa acgctgccat tccagatcgc catcatgaac agtatgggga
      421 atgatcagat ccgcactgtt aatctgatta aatctgcccg tgttggctat acaaagatgt
      481 tgctgggggg ggggcgggta ttttattgag cataaatccc gaaacagtct gctttttcag
      541 cccacggatt ctgccgctga agattttatg aagtctcacg tggaggcgac gattcggaac
      601 gtgccatgcc tgaaagacct ttccccatgg ctgggtcgta aacatcgtga caatactctc
      661 acgctgaaac gcttttcatc gggcgtcggt ttctggtgcc tgggcggcgc tgccgccaaa
      721 aactaccgtg aaaaatccgt tgacgtggtc tgctatgacg aactttcctc gttcgagccg
      781 gatgtcgaaa aagagggctc gccaaccctg ctgggggata agcgtattga ggggtcggtg
      841 tggccaaaat ccattcgcgg ctcgacgcct aaaatcaaag gcacctgcca gatcgaaaaa
      901 gccgctaacg agtcggcgca ttttatgcgt ttttatgtgc cctgcccgca ctgtggggag
      961 gcgcagtatc tgaaatttgg cgatgagtcc acgccttttg ggcttaaatg ggagaaggac
     1021 agccctgaaa gtgttttcta cctctgtgaa catcatggct gcgtgatcca tcagtctgaa
     1081 ctggaccaga gcaacgggcg gtggatctgt gaaaacacgg gcatgtggac ccgtgacggt
     1141 ctgacgtttt tcagcgcccg gggtgatgaa attccgccgc cgcgctccat catgttccat
     1201 atctggacgg cgtacagtcc gttcaccacc tgggtacaga ttgtctatga ctggctggat
     1261 gcactgaaag atcccaacgg cctgaaaacc tttgtgaaca ccacgctggg cgagacctgg
     1321 gaagaggccg tgggcgaaaa actcgatcac caggtactga tggataaggt ggtgcgttac
     1381 acggcggcgg tgcctgcccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac
     1441 cgttttgaga tgtatgtctg gggatgggct ccgggagagg aagcctttct ggtggataaa
     1501 atcatcatta tggggcgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc
     1561 aacaaaaaat accgccatgc ggatggcacc gaaatgacta tttcccgtgt ctgctgggac
     1621 accgggggga tcgatggtga aattgtttat cagagatcaa aaaaacacgg tgttttccgg
     1681 gtgctgccgg taaaaggcgc atctgtctat ggcaagccgg tgatcaccat gccaaaaacc
     1741 cgcaatcagc ggggcgtgta tctgtgtgaa gtgggaacgg acaccgcaaa agaaattctc
     1801 tatgcccgta tgaaagccga tccctcgcct gcggatgaag ccacgtcgta tgccatccgt
     1861 tttcctgatg atccggagat tttttcgcag acagaggcgc agcaactggt ggcggaagag
     1921 ctggtggaga agtgggaaaa aggaaagatg cgtctgctgt gggataacaa aaagcggcgt
     1981 aacgaagcgc tggactgcct ggtgtatgcc tacgcggcat tacgtgtgtc cgtgcaacgc
     2041 tggcagcttg atctggctgt actggcaaaa tcccgggaag aagagacgac ccggccaacc
     2101 ctgaaagaac tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca
     2161 ggcgctgcgt caggcgcgcc ttgatttatt aaccggtaaa cgggtggtgt ctgtccagaa
     2221 agatggtcgc agaattgaat atacggcggc ttctctggat gagcttaacc gggcgatcaa
     2281 tgatgcggag tcggtactgg ggacaacccg gcgtcgccgt cgtccgctgg gagtgaggtt
     2341 atgaaacgaa cgcctgtcct gattgatgtg aacggcgttc cgcttcgtga gagtctcagc
     2401 tacaacgggg gcggtgcagg atttggcggg caaatggcgg agtggttgcc accggcgcag
     2461 agtgccgatg cggccctgct gcccgcgttg cgtctgggga atgcccgggc agatgatctg
     2521 gtgcgcaata acggaatagc ggccaatgcg gtggcactgc ataaggatca cattgtcggg
     2581 catatgtttc ttatcagcta ccgtccgaac tggcgctggc tggggatgcg ggagaccgca
     2641 gcaaaaagct ttgtcgatga ggtggaggcg gcctggtcgg aatacgccga agggatgtct
     2701 ggcgagatcg acgtggaagg aaaacgcacg ttcacggaat ttatccgtga aggtgtgggc
     2761 gttcatgcgt ttaacggcga aatctttgtg cagccggtct gggatacgga aaccacgcag
     2821 ttattccgta cgcgttttaa agccgtgagt ccgaaacggg tggacacgcc aggacacggt
     2881 atggggaacc gttttctgcg ggccggggtg gaggtcgatc gatatggccg tgccgttgcg
     2941 taccatatct gtgaggatga ttttcctcgc tccgggagtg gacgatggga acggatcccg
     3001 cgtgaacttc ccaccgggcg tccggccatg ctgcatattt tcgagccggt ggaggacggg
     3061 cagacccgtg gggccaacca gttttacagc gtcatggaac ggctgaagat gctcgattcc
     3121 ctgcaggcaa cacagcttca gtcggccatt gtgaaagcca tgtatgcagc gacgattgaa
     3181 agtgaccttg ataccgaaaa ggcctttgaa tatatcgccg gtgcgccgca ggggcagaag
     3241 gataatccgc ttattaatat tctggagaag ttctccagct ggtatgacac gaataacgtg
     3301 acgctgggtg gtgtcaaaat tccgcacctt ttccccgggg atgatctgaa actacagact
     3361 gcgcaggatt cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatatcgcc
     3421 gccggtcttg gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttattca
     3481 agtgccaggg cctctgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt
     3541 gcggcccggc tggccacgca gatgttttcc tactggctgg aagaggcact tcttcggggg
     3601 attatccgtc cgccacgggc gcgttttgat ttttatcagg cgcgatcagc ctggtcacgg
     3661 gcagagtgga ttggtgccgg aagaatggcc attgacgggc tcaaggaggt tcaggaatcg
     3721 gtgatgcgca ttgaggccgg actgagcacg tatgagaaag agccggcgct gatgggcgag
     3781 gattatcagg acattttccg ccagcaggtc agggaatctg cagagcggca aaaagccgga
     3841 ctctcacgtc cggtgtggat agcgcaggcg tatcagcagc agatagcgga gagtcgcagg
     3901 ccggaagagg agacaacacc ccgtgagacg taatctttca cacattattg ccgcagcatt
     3961 caatgaaccg ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg
     4021 cgagatgggg gcagcaagtc tttcggtacc acagcagcag gtacagcttg atgctcccgg
     4081 aatgctggct gaaacggacg agtacatggc cggaggtaaa cgaccggccc gtgtttaccg
     4141 ggtggtgaac ggtattgctg tactgccggt gaccggcacg ctggtgcacc ggctgggggg
     4201 tatgcggcca ttttccggaa tgacaggcta tgacggcatt gtcgcctgtc ttcagcaggc
     4261 aatggcggat agccaggtgc ggggcgtact gctggacatt gacagtccgg gcgggcaggc
     4321 cgccggcgcg tttgactgcg ctgacatgat ttaccgcctc cgtcagcaga agccggtctg
     4381 ggcactgtgc aatgacacgg cctgttctgc agccatgctg ctggcgtcgg cctgctcccg
     4441 acggctggtt acccagacat cccgtatcgg ctccattggc gtgatgatga gccatgtcag
     4501 ctatgccggt catctggcgc aggccggtgt ggatatcacg ctgatttact caggggcgca
     4561 caaggtggat ggcaatcagt ttgaagcctt accggcagag gttcgccaga acatgcagca
     4621 gcgcattgat gcggcgcgcc ggatgtttgc cgaaaaagtg gccatgttta ccggtctgtc
     4681 tgttgatgcc gtcacgggaa cagaggccgc cgtttttgaa ggtcagtccg gcattgatgc
     4741 cgggctggcg gatgaattag tcaatgcgtc ggatgccatc agtgtgatgg ccacggcgct
     4801 gaacagtaat gtcagaggag gcactatgcc gcaattaact gcaacggaag ccgccgcgca
     4861 ggagaaccag cgagtgatgg ggatcctgac atgccaggaa gcgaaaggac gtgaacagct
     4921 tgccacgatg ctggcaggac aacagggcat gagcgttgaa caggcccggg cgattctggc
     4981 cgcggcggca ccgcagcagc cggtggcatc cacgcagagt gaagccgatc gcattatggc
     5041 gtgtgaagaa gcgaacggtc gtgaacaact ggcggcaacg ctggcggcga tgccggagat
     5101 gacggtggaa aaagcccgcc cgatcctggc tgcttcaccg caggcggatg ccggaccctc
     5161 actccgtgat cagatcatgg cactggatga ggcaaaaggg gctgaggcgc aggctgaaca
     5221 gctggctgcc tgcccgggaa tgactgtgga gagcgcccgg gctgtgctgg ctgcgggatc
     5281 aggtaaggca gaaccggtct ctgcatccac aaccgccctg tttgaacgca tcatggcgaa
     5341 ccattcaccg gctgcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga
     5401 cgtgaaaatg ctcatggcca tgccatgaag tcagtgctga ccatcaacag gaggttttta
     5461 caatatggta acgaaaaaca tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa
     5521 tgatccggct cataccgcca caggcagcag cgggatttcc tcggcaacac cggcactgac
     5581 gcccctgatg ctggatgaag ccagcgggaa actggtggtc tgggacggac agaaagccgg
     5641 tagtgcagtt ggcatactgg tactgccgct tgaaggcaca gagacggtac tgacctatta
     5701 caagtcgggg acctttgcga cggaggcaat ccgctggcct gaaagtgtgg atgaacacaa
     5761 aaaggcaaat gcctttgccg gcagtgccct gagtcacgcg gcgctgccgt aacacgttat
     5821 caggccaccg cggtggcctg actgatttct gaatgaaagg aactgattta tgggattgtt
     5881 tacgacccgc cagttactcg gttataccga acaaaaagtg aaatttcgtg cgctgtttct
     5941 ggagctgttt ttccgccgta cggtgaattt ccataccgaa gaggtgatgc tggacaaaat
     6001 taccggaaaa acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aagtgctgcg
     6061 tcatcgcggt ggtgaaaccc gcgtgttacg tccgggctac gtcaagccga aacacgaatt
     6121 cccctggagc cggtaaaagg agccggtacc accctgtggg tttataacgg tcagggtgac
     6181 gcctatgcaa acccgttgtc agacgatgac tggcagcgac tggctaaggt gaaggatctg
     6241 acgccgggcg agatgacggc agaatcctac gatgataact acctggatga tgaagacgcg
     6301 gactggaccg cgaccgggca ggggcagaaa tctgcaggtg ataccagttt tacgctggcc
     6361 tggaaaccgg gagaggaagg ccagaaaggg cttataggct ggtttgaaag cggcgatgtc
     6421 cgggcctata aaatccgttt tccgaatggc acggtggatg tgtttcgtgg ctgggtcagc
     6481 agtatcggta aggccgtgac ggcgaaagaa gtgatcaccc gcacggtgaa agtcactaac
     6541 gtgggtaaac cttctgtagc ggaagaacgc agcaaaatta cgccggtcag tgcgattaag
     6601 gtgacgccga catccggtac ggtggcaaaa gggaaaacaa ccaccctgac ggtttctttt
     6661 gagccggaaa gtgcaacaga caagacgttc agagcggttt ccgccgatcc gtcgaaagcc
     6721 accattagtg tgaaagatat gacaattacg gtaaacggcg tggcgacagg taaggtgcag
     6781 atccctgtgg tgagcggaaa tggtcagttc gccgcagtgg ctgaagtcac cgttactgaa
     6841 gcgggcgctg cagggtaaac ggaggtcata catgtttctg aaaacagaac aatttgaata
     6901 taacggtgtg tctgtcacgc tttccgaatt gtctgcgctg cagcgtatcg agcatcttgc
     6961 cctcctgaaa cggcgtgcag aacaggcaga atccagcggc aacctgcagg taagcgtgga
     7021 agatctcgtc agaaccggcg cgtttctggt ggcgatgtcc ctgtggcata accatccaca
     7081 gaaaacgcag tcaccgtcaa tgaatgaggc cgtgatgaag atagagcagg aagtgctcac
     7141 cacctggcct gccgatgcca ttgcccgggc ggaagacgtg gtgttgtgcc tgtccgggat
     7201 gagcggggct gttcgtccgg atactgatat tactgaagtg gcgaaaaata acacgctgac
     7261 tgatgatgat ttttctgcgg gaaagtcttc gacggcgagc tgaactttgc cctcagactg
     7321 gcgcgtgaga tggggagacc cgactggcgc gccatgcttg ccgggatgac atccaccgaa
     7381 tatgccgact ggcaccgttt ttaccgcacg cattattttc aggataccca gctggatatg
     7441 catttttccg ggctgacgta cgctgtactc agcctgtttt tttgcgatcc ggatatgcat
     7501 ccctctgatt tcagtctgct tgtcccccgg catgaggaag agcaggtgga gaggccggat
     7561 gaggacaaaa tgctgatgca gaaagcggca ggacttgccg gaggcgtccg gttcggtggg
     7621 gacggagggc gcgatatttt atcgtctgcg gatgtggcgg atgtcatggt ggatgatgcc
     7681 gcattaatga tggcttcagc ggggattccg ggaggtgtga gatatgtccc agccggttgg
     7741 tgatcttgtt attgacctga gtctggatgc tgtccgtttc gatgagcaga tgagccgggt
     7801 aaggcgtcat ttttcaggtc tggataccga cgtcagaaaa accgccagtg ctgttgaaca
     7861 gggcctgagc cgccaggcgc tggctgcaca aaaagccggg atttccgtcg ggcagtataa
     7921 agcggccatg cgaaccctgc ccgcacagtt tacggatatc gccacgcagc ttgccggtgg
     7981 tcagaatccc tggctgatcc tgctgcaaca gggcggtcag gtgaaggact ccttcggcgg
     8041 gatgatcccc atgttcaggg ggcttgccgg tgcgatcacc ctgccgatgg tcggggtcac
     8101 ctcgctggcg gtggcgacag gtgcgctggt gtacgcctgg taccagggag attccacgct
     8161 ttcagcgttt aataaaaccc tggttctttc cggtaatcag tccggactga ctgccgatcg
     8221 catgctgacg ctctcaagag ccgggcaggc agcagggctg acgtttaacc aggcgagaga
     8281 gtcactggca gccctggtga atgccggtgt gcgtggtggt gaacagtttg atgccatcaa
     8341 ccagagtgtc gcgcgttttg cttctgcatc cggtgtggag gtggacaagg ttgcagaggc
     8401 tttcggaaaa ctgaccaccg accctacgtc ggggctgatt gcgatggtgc gccagttccg
     8461 taacgtgacg gcagagcaga ttgcgtatgt tgcgcagctg cagcgttccg gtgatgaggc
     8521 cggggcctta caggcggcga acgatatcgc cacgaaaggc tttgatgagc agacccgtcg
     8581 cctgaaagaa aacatgggga cactggagac ctgggcggat aaaaccggga aggcattcaa
     8641 atcgatgtgg gatgccattc tggatatcgg tcgtcctgag tcctcagcgg atatgctcgc
     8701 cagtgcacag aaggcatttg atgaggcgga taaaaaatgg cagtggtacc agagccggag
     8761 ccagcgccgg ggaaaaaccg cctctttccg ggccaacctt cagggcgcat ggaatgaccg
     8821 ggaaaatgcc cgtctggggc tggcagcggc cacgctgcag tcggatatgg aaaaagccgg
     8881 tgaactggcc gccagggacc gggccgaacg ggacgcatca cagctgaagt ataccggaga
     8941 ggcgcagaag gcgtatgagc gtctgctgac gccgctggag aaatataccg cccgtcagga
     9001 agaactgaat aaggccctga aagacgggaa aatcctgcgg gcggattaca acacgctgat
     9061 ggcggcggcg aaaaaggatt atgaatcgac gctgaaaaag ccgaagtcgt caggagtcaa
     9121 agtgtcagcc ggtgagcgtc aggaagacca ggcgcatgct gccctgctgg cgcttgaaac
//
LOCUS       DAZKFZ010000186         9158 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000186 DAZKFZ010000000
VERSION     DAZKFZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..362
                     /gene="pgaA"
                     /locus_tag="ACHOQW_003796"
                     /gene_synonym="hmsH"
                     /gene_synonym="ycdS"
     CDS             <1..362
                     /gene="pgaA"
                     /locus_tag="ACHOQW_003796"
                     /gene_synonym="hmsH"
                     /gene_synonym="ycdS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="outer membrane protein essential for the synthesis
                     of poly-beta-1,6-N-acetyl-D-glucosamine (PGA); adhesin
                     required for biofilm formation; PgaA (HmsH) seems to
                     translocate and/or dock PGA to the cell surface; member of
                     the hmsHFRS (ycdSRQP or pgaABCD in Escherichia coli)
                     operon in Yersinia; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP9574794.1"
                     /translation="SSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLL
                     WRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYD
                     GDREHNLYVEFDMTFRF"
     gene            371..2389
                     /gene="pgaB"
                     /locus_tag="ACHOQW_003797"
     CDS             371..2389
                     /gene="pgaB"
                     /locus_tag="ACHOQW_003797"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP9574795.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            2382..3707
                     /gene="pgaC"
                     /locus_tag="ACHOQW_003798"
     CDS             2382..3707
                     /gene="pgaC"
                     /locus_tag="ACHOQW_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP9574796.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            3709..4122
                     /gene="pgaD"
                     /locus_tag="ACHOQW_003799"
     CDS             3709..4122
                     /gene="pgaD"
                     /locus_tag="ACHOQW_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP9574797.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(4172..4960)
                     /gene="phoH"
                     /locus_tag="ACHOQW_003800"
     CDS             complement(4172..4960)
                     /gene="phoH"
                     /locus_tag="ACHOQW_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP9574798.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(5580..6851)
                     /gene="efeB"
                     /locus_tag="ACHOQW_003801"
     CDS             complement(5580..6851)
                     /gene="efeB"
                     /locus_tag="ACHOQW_003801"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP9574799.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(6857..7984)
                     /gene="efeO"
                     /locus_tag="ACHOQW_003802"
     CDS             complement(6857..7984)
                     /gene="efeO"
                     /locus_tag="ACHOQW_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP9574800.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(8042..8872)
                     /gene="efeU"
                     /locus_tag="ACHOQW_003803"
     CDS             complement(8042..8872)
                     /gene="efeU"
                     /locus_tag="ACHOQW_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP9574801.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         2379 a         2289 c         2155 g         2335 t
ORIGIN      
        1 ggtcttcacc atatttgatt gtcgatttcc tacccagtct gtattacgaa caaaatacag
       61 aacacgatac cccatactac aaccctataa aaacgttcga tattgttccg gcatttgagg
      121 caagccattt gttatggcga agctatgaaa atagctggga gcaaatattc agcgcaggtg
      181 ttggtgcctc ctggcaaaaa cattatggca cggatgtcgt cacccaactc ggctacgggc
      241 aacgcattag ctggaatgac gtgattgatg ctggcgcaac gctacgctgg gaaaaacgac
      301 cttatgacgg tgacagagaa cacaacttat acgttgaatt cgatatgaca ttcagatttt
      361 aaggataaat atgttacgta atggaaataa atatctcctg atgctggtga gtataattat
      421 gctcaccgcg tgcattagcc agtcaagaac atcatttata ccgccacagg atcgcaaatc
      481 tttactcgcc gagcaaccgt ggccgcataa tggttttgta gcgatttcat ggcataacgt
      541 tgaagacgaa gctgccgacc agcgttttat gtcagtgcgg acatcagcac tgcgtgaaca
      601 atttgcctgg ctgcgcgaga acggttatca accggtcagt attgctcaaa ttcgtgaagc
      661 acatcgagga ggaaaaccgc taccggaaaa agctgtagtg ctgacttttg atgacggcta
      721 ccagagtttt tatacccgcg tcttcccaat tcttcaggcc ttccagtggc ctgctgtatg
      781 ggcccccgtc ggcagttggg tcgatacgcc agcggataaa caagtaaaat ttggcgatga
      841 gttggtcgat cgagaatatt ttgccacgtg gcaacaagtg cgagaagttg cgcgttcccg
      901 gctcgttgag ctcgcttctc atacatggaa ttctcactac ggtattcagg ctaatgccac
      961 cggcagctta ttgcctgtat atgtaaatcg tgcatatttt actgaccacg cacggtatga
     1021 aaccgcagca gaataccggg aaagaattcg tctggatgct gtaaaaatga cggaatacct
     1081 gcgtacaaag gttgaggtaa atccacacgt ttttatttgg ccttatggcg aagcgaatgg
     1141 catagcgata gaggaattaa aaaaactcgg ttatgacatg ttcttcaccc ttgaatcagg
     1201 tttggcaaat gcgtcgcaat tggattccat tccgcgggta ttaatcgcca ataatccctc
     1261 attaaaagag tttgcccagc aaattattac cgtacaggaa aaatcaccac aacggataat
     1321 gcatatcgat cttgattacg tttatgacga aaacctccag caaatggatc gcaatattga
     1381 tgtgctaatt cagcgggtga aagatatgca aatatcaacc gtgtatttgc aggcatttgc
     1441 tgatcccgat ggtgatgggc tggtcaaaga ggtctggttt ccaaatcgtt tgctaccaat
     1501 gaaagcagat atttttagtc gggttgcctg gcaattacgt acccgctcag gtgtaaacat
     1561 ctatgcgtgg atgccggtat taagctggga tttagatccc acattaacgc gagtaaaata
     1621 cttaccaaca ggggagaaaa aagcacaaat tcatcctgaa caatatcacc gtctctctcc
     1681 tttcgatgac agagtcagag cacaagttgg catgttatat gaagatcttg ccggacatgc
     1741 tgcttttgat ggcatattgt tccacgatga tgctttgctt tcagattatg aagatgccag
     1801 tgcaccggct atcacggctt atcagcaagc aggctttagc gggagtctga gcgaaattcg
     1861 acaaaacccg gagcaattta aacagtgggc ccgctttaaa agtcgtgcgt taactgactt
     1921 cactttagaa cttagtgcgc gcgtaaaagc cattcgcggt ccacatatta aaactgcacg
     1981 aaatattttt gcacttccgg taatacaacc tgaaagtgaa gcctggtttg cacagaatta
     2041 tgctgatttc ctaaaaagct atgactggac cgctattatg gctatgcctt atctggaagg
     2101 tgtcgcagaa aaatcggctg accaatggtt aatacaattg accaatcaaa ttaaaaacat
     2161 ccctcaggct aaagacaaat ctattttaga attacaggca caaaactggc agaaaaatgg
     2221 tcagcatcag gctatttctt cgcaacaact cgctcactgg atgagcctat tacaactgaa
     2281 tggagtgaaa aactatggtt attatcccga caattttctg cataaccaac ctgaaataga
     2341 ccttattcgt cctgagtttt caacagcctg gtatccgaaa aatgattaat cgcatcgtat
     2401 cgttttttat attatgtctg gtgttatgca tacccctatg cgtagcgtac tttcactctg
     2461 gtgaactgat gatgaggttc gttttcttct ggccgttttt tatgtccatt atgtggattg
     2521 ttggcggcgt ctatttctgg gtctatcgtg aacgccgctg gccgtgggga gaaaacgcac
     2581 cagctcccca gttgaaagat aatccgtcta tctccattat cattccctgt tttaatgagg
     2641 agaaaaacgt tgaggaaacc atacacgccg ctttagcaca gcgttatgag aacattgaag
     2701 ttattgccgt aaatgacggt tcaacagata aaacccgtgc catcctggat cgcatggctg
     2761 cacaaattcc ccatttgcgg gtcattcatc tggcgcaaaa ccaggggaaa gccattgcgc
     2821 ttaaaaccgg agctgccgcg gcgaaaagtg aatatctggt gtgcattgat ggcgatgcgt
     2881 tattagaccg cgatgcggcg gcatatattg tggaaccgat gttgtacaac ccgcgtgtgg
     2941 gtgccgtaac cggtaatcct cgtattcgaa cacgttctac cctggtgggt aaaattcagg
     3001 ttggcgagta ttcctcaatt attggtttga tcaagcgaac ccagcgtatc tatggaaacg
     3061 tatttaccgt ttccggtgtt attgccgcat ttcgtcgcag cgccctggca gaagtgggtt
     3121 actggagtga cgatatgatc accgaagata ttgatattag ctggaagctg cagttgaatc
     3181 agtggacgat tttttacgag ccacgggcac tgtgctggat attaatgcct gaaacgttaa
     3241 aagggctgtg gaaacagcgc ctgcgctggg ctcagggcgg tgcagaagta ttcctcaaaa
     3301 atatgacaag gttgtggcgc aaagaaaact ttcgaatgtg gccgctgttt tttgaataca
     3361 gcctgacgac aatatgggcc ttcacctgcc tggtcggttt cattatttac gcagtccaac
     3421 ttgccggtgt accgttaaat attgaattga cacatatcgc tgcgacacat actgccggaa
     3481 tattattgtg tacgttatgt ttactgcaat ttattgtcag cctgatgatc gagaatcgct
     3541 atgagcataa tctgacttca tcgcttttct ggattatttg gttcccggtt attttctgga
     3601 tgctgagcct ggcaacgaca ttggtatcat ttacacgagt catgttgatg cctaaaaagc
     3661 aacgcgcccg ttgggtaagt cccgatcgcg ggattctgag aggttaatat gaacaattta
     3721 attattacga cccgacaatc accagtacgt ttactggttg attatgttgc cacaaccatc
     3781 ttgtggacat tatttgcgtt gttcatattc ttattcgcca tggatctgct gacgggttat
     3841 tactggcaaa gcgaggccag aagccgactt cagttctatt ttttgctggc agtggcgaat
     3901 gccgtcgtgt taattgtctg ggcgctgtac aataagctgc gttttcaaaa acagcagcat
     3961 catgcagcct accaatatac gccgcaagaa tatgcagaga gcttagcaat acctgatgag
     4021 ctctatcagc aactacaaaa aagccacagg atgagcgtac acttcaccag ccaggggcaa
     4081 ataaaaatgg ttgtttcaga aaaagcgcta gtcagggcat aaacacccaa aacaaagccc
     4141 ggttcgcccg ggctctgcac cgataataca cttaactgta tgcatgcagc gtacgttggc
     4201 aaagtgccga acgtacgcaa tcctcttttc cgaaccggac gatcccaacc atttcatctt
     4261 cttcgaaacg ttccagcgcg tcacttaatc cggagcacac gccgcgaggc aaatcgcatt
     4321 gcgtgatatc accgttgacg ataaccgtca cgttctcccc gaggcgggtt aaaaacattt
     4381 tcatttgcgc ggcagtcaca ttctgcgcct cgtcaagaat gacgactgca ttttcaaagg
     4441 tacgtccacg catataggcg aacggcgcaa tttccacctt ccctatttcc ggtcgcaggc
     4501 agtactgcat aaaggaagcc cctaagcgcc ggaccagcac gtcgtagacc gggcgaaaat
     4561 agggagcaaa cttttctgcg atatctccag gtaagaagcc aagatcttca tcggcttgca
     4621 gaactggacg ggtgacgata atcctgtcga catccttatg tatcagggcc tctgccgctt
     4681 ttgctgcgct gatccaggtt tttccgcacc cggcttcgcc cgtggcgaat atcagctgct
     4741 tactctcaat agccttcaga tagtgcaatt gcgcttcatt tcgcgcgagg atgggcgaag
     4801 tatcgcgact gtcgcgggcc ataccaatgg cttctacgcc gcccatctgc acaagcgagg
     4861 tgaccgattc ttcttcacgc tgcttatggc tgcgcgaatc ccgtctcagc acacgttttg
     4921 cctcgcgacg agctttgatc actgcttttt gtcttcccat ggagagcacc ttgagttgtt
     4981 tgtattcatc acacgcgccg ttggcagcgc gattatgcgc acgaacatca gagggttggc
     5041 ttccttgtaa gccatagttt gcttttggat aaaatgccga aaaacggcta cgcgcaccgt
     5101 ttacggcgtc ggtaacacat gaaaagaaag gatgaggttg aaaatgcaaa gtgacgagat
     5161 gactaccgga ggagaaaact ccgcgagtgg tggcgcgttg attatctaaa acatgtccag
     5221 tacaggacgt taccatccgc gatctccata gtgactgact atcactgccg ggaacttccg
     5281 ctgctactta ataagtacaa cagttctcgc atttattgca acaatatatt tacttatatt
     5341 taactataaa acaccatttc agtgacatta gtttctactg gaaagatgac agagtgatga
     5401 cagatatgaa aaaagctgtg tgctttcagc aggatttgca tcaggcatca gtgcacgatc
     5461 gccgtatgcg gcgtgaacgc cttatccgcc taccgaaccc gcaccgattg taggcataag
     5521 aagacgcgca agcatcgcat caggcatctg cgcaagaacg ccttatccgt ctaaaaacat
     5581 taaacccgca ataacgcgct tccgagataa tcgttcgcgt ccttcactcc cggcagcgca
     5641 aaaaaataac cgccgccgat aggtttaacg tattcctcca gcgcttcgcc attgagcctt
     5701 ttttgtactg tcaggaagcc tttttccaga tcgtgttggt aacagacaaa cagtagcccc
     5761 atatccagtt gcccggagtt ggtgacgccc agtgaataac tgtagccacg acgcagcatc
     5821 aggctggact cactctccgc cgtgcgggga ttcgccagcc ggatatggct gtccagcgca
     5881 atccccttcc cttccgggtc gctggcgtaa tcaggcacat catgctcatg ctgcattccc
     5941 agcggcgcac cggtttgctt atcacggcca aaaatcgtct gctgttcttt cagcggcgtt
     6001 ctgtcccaaa attccactcg aaactgaatc aagcgtactg cctgatagct gccaccgatt
     6061 gtccacgcag gctcctgctg atctgccgtt acccacacaa ctttttgcat caacttatca
     6121 ttctggctat cgggattggc agtgccgtct ttgaaaccca gcaaattaat cggcgtctct
     6181 ttgcctttac tacgcgccgc gtgatcggaa ataaaccctt cccgcttcca gcgcacgctg
     6241 agcaaatccg gcgtatgttt gatgatatcg cgcagtgcat ggatcaccgt gtcctgggtg
     6301 ttggcgcaaa tttgtagcaa cacatcacca tgacataacg ccgcatccag cgagtcgttg
     6361 ggaaaacgcg tcatcttctg caactttttc ggcatctgtg gcgcaaggcc aaagcgctca
     6421 tcaaacaatg agtgacccac cgataacgtg atggtgagat tatcgggcgc aatgtagccg
     6481 ccaagaatgc cggaatcgag tggtggcagg cgcggatttg gcgtttctgg tgccgctccg
     6541 ccctgagtca gaaaagcaaa acgctgagtc aacaagcgaa acaaccgttc aagatcggct
     6601 ttatcgctgg caagcacatc aaacgccacc agcatcattg cggcctgttg tggcgtcagg
     6661 atccctgcct gatgctcacc ataaaacggc tgtttctcat tgcgtgcaac cggtgaaagc
     6721 gtacccggcg cactttgcgt tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc
     6781 agcgcaccaa tgcctttcag taaacgtcgg cgtgacggtt cattcacgcc gtttttatct
     6841 tcatactgca taatgcttaa tccaatccca gcacaccgcg aagttgcgcc agatcttccg
     6901 ccagcgcagt aatcggtcct ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg
     6961 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     7021 catcgacttt tgccaacagt tccgggttag ctttttgcag ttgtggacgc agcagatcga
     7081 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     7141 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcaccgc
     7201 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     7261 cggtataaag ctggtcagcg tactgatcca tgcctttggt ggtgttgtcg ccaaacaatg
     7321 ctttttccag acggtggaaa ccggtgaatt ttggatcagc ggctttttgc tcgtaatcat
     7381 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatgc
     7441 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     7501 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg tgtggtttcc gccatgacat
     7561 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
     7621 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
     7681 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaag ccaggggcga
     7741 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccattcc agcgccttct
     7801 ggctgtggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
     7861 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
     7921 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
     7981 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
     8041 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
     8101 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggcgct tcctgatagc
     8161 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagagcacc gcactcatat
     8221 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
     8281 cagctgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
     8341 caagattgag gcgaataccg ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa
     8401 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
     8461 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
     8521 cccagccatg atgatttcca cgctgcaatg cgctatcgac agcctgctcc agttgtactt
     8581 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
     8641 tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg ccggtggttt
     8701 cgttaatgaa gatccccaag cccaggcaca acgcagcggc aagcaacacg ccaatccaca
     8761 tcacaccaat ccatcggcct cgctgggtac gcttaagata gctggcaatc aaactgacaa
     8821 tcagcgcggc ttcaagtcct tcgcgcaaca taatgagaaa cggaacaaac atgccaccca
     8881 cccttaaacg tttttctgag tcaatctatg caaagaaaag taaatcgcag tgatagcgat
     8941 tatcattacg ctaaagaaaa atacaagcgg aatatttata aaatgatgtt ggaatgtaaa
     9001 aatgaggaga tcaaatccgg agaaaaaagc gattatttta aggtatttag ctgacgtttc
     9061 gcccggcaga tgaagcttgt acgaatgttg gtggcgacaa actcgctgca gcgtgcccga
     9121 agggttgaat tttcagttag tcgtttcaca aaagaacg
//
LOCUS       DAZKFZ010000187         9105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000187 DAZKFZ010000000
VERSION     DAZKFZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(344..1537)
                     /gene="tyrB"
                     /locus_tag="ACHOQW_003804"
     CDS             complement(344..1537)
                     /gene="tyrB"
                     /locus_tag="ACHOQW_003804"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP9574802.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1790..2869)
                     /gene="alr"
                     /locus_tag="ACHOQW_003805"
     CDS             complement(1790..2869)
                     /gene="alr"
                     /locus_tag="ACHOQW_003805"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9574803.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2922..4337)
                     /gene="dnaB"
                     /locus_tag="ACHOQW_003806"
     CDS             complement(2922..4337)
                     /gene="dnaB"
                     /locus_tag="ACHOQW_003806"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9574804.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4420..5403
                     /gene="qorA"
                     /locus_tag="ACHOQW_003807"
     CDS             4420..5403
                     /gene="qorA"
                     /locus_tag="ACHOQW_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP9574805.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5569..5811)
                     /gene="pspG"
                     /locus_tag="ACHOQW_003808"
     CDS             complement(5569..5811)
                     /gene="pspG"
                     /locus_tag="ACHOQW_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP9574806.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5945..6982)
                     /gene="dusA"
                     /locus_tag="ACHOQW_003809"
     CDS             complement(5945..6982)
                     /gene="dusA"
                     /locus_tag="ACHOQW_003809"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP9574807.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            7071..8168
                     /locus_tag="ACHOQW_003810"
     CDS             7071..8168
                     /locus_tag="ACHOQW_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9574808.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            8230..8478
                     /locus_tag="ACHOQW_003811"
     CDS             8230..8478
                     /locus_tag="ACHOQW_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9574809.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(8489..8608)
                     /locus_tag="ACHOQW_003812"
                     /pseudo
     CDS             complement(8489..8608)
                     /locus_tag="ACHOQW_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(8639..>9105)
                     /locus_tag="ACHOQW_003813"
     CDS             complement(8639..>9105)
                     /locus_tag="ACHOQW_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9574810.1"
                     /translation="LNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEML
                     ENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMNS
                     NVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
BASE COUNT         2464 a         2293 c         2140 g         2208 t
ORIGIN      
        1 tttaacttta gaatttaatg atactgaaat tgaatgtcag aaagtcacag gcaataaggc
       61 aaagcatatt cttgaggata tagaaaaatt ctatcgtaac aaacccgata cttattatta
      121 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaaa cagggaaagg
      181 aattccagat aattgggata aacttaacaa taaaagaact aacaacgttt attatctctc
      241 acgtagaacg atggtatcaa aaaaatcatc tataaagaaa gagtgaaagc gacgacagtt
      301 taatttcact gcaggctggg taattccagc ctgctttcct gcattacatc accgcagcaa
      361 acgcctttgc cacacgttgc acatttgccg tatttaaccc ggcgacacac atgcgaccgc
      421 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
      481 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
      541 ctgtgcttaa taccttcacc agttcctgac gcattgccag aatgcgagta cgcatctctt
      601 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
      661 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
      721 gtacgcggcc tgcagcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
      781 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
      841 caatggcgcg aatggcgtag gcatcctctt ccataccggc accaaatcct tgataggcaa
      901 tatcaaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactggt
      961 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     1021 gggcaggtaa tgttttcagc atcgccaaca ggtcattaaa gcgcacgccg ttagtcgctt
     1081 cgtcatacca ggggtaagtg cttacttcga atccagcccc ggcgaatatt gctacgtggt
     1141 tttcccaggt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     1201 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     1261 gttgcagtac cggatggtcg gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     1321 taagcccttc catcggtaaa taaagcgaag cgccatgagg caccgcattc aggcgcgctt
     1381 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     1441 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     1501 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     1561 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggggc
     1621 gaacggcaat tagcaacaga gtgagactca tgacaaacgt acatccgcca gaggcacgac
     1681 ctttataaga gcatgaaaga atatcaactt attgaattgg taggatttta ttggccggat
     1741 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     1801 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     1861 tttcagcgat acgttctacg ggcaaacctt caccccacaa aatgaccggg tccccggctt
     1921 tatcctgtgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     1981 tcggcacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     2041 catcgccata gcccatcgca actacaccaa gacgagtatc acgttcgctt atccaggttc
     2101 caccataacc gacaggctct ccgactttat gctcacgcac ggcaatcagg ctggaggtta
     2161 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     2221 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     2281 tgccacccga cgcggcaatg gaacgttgac ctggtttgcc ttcgcaaaag gtattaaaga
     2341 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     2401 cgatattcac cggctgccga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     2461 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     2521 ccggctcgtc caggctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     2581 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     2641 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     2701 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     2761 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     2821 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     2881 gataagtgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     2941 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     3001 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     3061 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     3121 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     3181 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     3241 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gtgccggtac gcgcatcagt
     3301 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     3361 cgggaacgca cttccgttgg cgtcaggccg gaggagtcat cgatatagat attgcgtttt
     3421 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     3481 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     3541 tctgatggca tctccagcga gaaaataagt accggtttat cctgcaacat cgccgcgttt
     3601 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     3661 aaatccgacg gctgcaagcc agcggttttt ttgttgagat catcataacc ggtgtttacc
     3721 ccggtaacgc catcgtgtgg ctgctgaaat aactgctcaa tacgcgccac ggttgcgtcg
     3781 agcacatcgg cgatgttctt cggcccttcg tctttgttcg cacgactttc ggcaatttta
     3841 aagacgcggg attcagccag atccagcaga tcttcgctgg tacgcccctg cggatcaaaa
     3901 ccagcttcgg caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
     3961 acgatgtccg cataggaact gatgttcgca gcacttggcg tattttttga caactctgcc
     4021 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccagcga ttccgcaagg
     4081 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
     4141 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
     4201 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
     4261 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
     4321 ggtttatttc ctgccatagt gaatggagtt accgagataa agaatgggtc gaaactttac
     4381 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
     4441 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
     4501 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
     4561 cgcagcggcc tttacccgcc gccatcacta cccagcggat taggtaccga agcggcaggc
     4621 atcgtgagta aagtcggcag tggtgtaaag catattaagg caggcgatcg tgtagtctat
     4681 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
     4741 attctgcctg cggcaatttc ttttgagcaa gctgcagcat ctttcctgaa aggcttaacg
     4801 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
     4861 gcagcagctg gcggcgttgg cttgattgcc tgccagtggg caaaagccct gggcgcgaaa
     4921 cttatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc gggcgcgtgg
     4981 caggttatta actatcgtga agagaatctg gtcgagcggt taaaagagat caccggtggt
     5041 aaaaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
     5101 tgcctgcaac gccgcggctt aatggtgagt tttggcaact catcaggtgc ggttaccggt
     5161 gtgaacttag gcattctcaa tcagaaaggc tcgttgtatg tgacacgccc ttccctgcaa
     5221 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
     5281 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
     5341 cagcgtgcgc atgagattct ggaaagccga gcgacgcaag gttccagcct gttgattcca
     5401 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
     5461 ggtacagcac gatgaatctg ttagaggcgc aatagtgaca gatttgatta tcaattccta
     5521 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
     5581 gcggtcataa cgctgatatt tcggcacttt tggtgcttta atcgccttaa taacccacac
     5641 caccgcaatc gccaggagta accacggcag caacttaatc atcaatgcca gcataccgcc
     5701 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
     5761 ggtgaccatc aacatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
     5821 ccaaatatga aatctcttgc aggcattaca agaatcatgc caaaaataat ctattgattt
     5881 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
     5941 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtctgcac
     6001 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
     6061 ggaacaagcc caacatatgc cgggtaatat ggccaagata cgtcccctgg ctgagttcac
     6121 gctcaatgta cggatacatg gcgcgcacta ccgccaccgg atcggcatcg gtatccgagg
     6181 aaccaaagat ctctcggtct accgccgcca gaatacccgg attctgatac gcttcgcgcc
     6241 cgaccatcac gccatccata tgttgcaaat gcgctttagc ctcttccagc gacttgatac
     6301 caccgttaat cgacatcgtc agatgcggaa agtcacgctt cagttgatac acacgcggat
     6361 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
     6421 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtgttg atgaaatcgc
     6481 agagaaattc atagctgtcc tggtcatcga tgccaatacg cgttttcacc gtcaccggaa
     6541 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
     6601 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
     6661 tctcatcata tccacgcgct tctgccagct tcgcacactg cgccagcgcc gccggatcgc
     6721 taccgccgag ttgcaacgct accggatgtt cttcttcact gtacgccagg taatcacctt
     6781 taccgtgaat aatcgcccct gtagtcacca tttcggtata cagcaacgta ttgcgggaaa
     6841 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacgctaa
     6901 accgagaatt ccagtaaaca ccagtttttt caggcatcac gctggtttga ttaatttttt
     6961 gtgcttcatg attatcgtgc atttttgaac atttcaggct atttttctcg cgttaggttc
     7021 ccgcacaggt tcccacgttt tatgggaacc cgaaataacg aggtcgtgta atggcgtact
     7081 ataacataga gaaacgacta aaatccgatg gcacaccacg ctatcgctgt aatgtgatta
     7141 tcaaagaaaa aggtgttatc acttacaggg aaagcaaaac attccctaaa catgctcatg
     7201 ccaaaacatg gggcacacag aaagtgatgg aattagatct atatggcatt ccatcatcaa
     7261 atgcagttga cggacttaca gtccgtgact tactacacaa atatttaaat gacccaaatg
     7321 ccggaggtaa agcaggccgt actaaaagat atgtgctgga actgcttatg gatagtgaca
     7381 tatccgcgat caaactatct gaactgacag aaaatgacgt aattgaacat tgcaggctaa
     7441 gaaacaacgc tggtgcaggc ccagcaacag tcagccacga tgttagttat cttggcagtg
     7501 ttctggatgc ggcaaaacct gtatacggaa tcaattacac atcaaacccg gcgaaaagcg
     7561 ctcgtccata tctacttaaa ctcggtttga ttggtaaatc aaaccgtcgt aatcgtagac
     7621 cagcatctga tgaacttgac atgctcattg aaggccttca acaacgatct actcataaat
     7681 gctcaaaaat tccgttcgtt gatatcctca aattttctgt gtggtcctgt atgcgaatcg
     7741 gagaagtatg ccggttacga tgggaagatc tcgaccaaga acaaaaatct atactagtaa
     7801 gagataggaa agatccacgt aaaaaggaag gtaaccatat gaaagttgcc ttgcttgggg
     7861 aagcctggga tatcgtccag cgacaaccaa aaaaatcaga attcattttt ccatataaca
     7921 gcacttctgt taccgcagga tttcagaggg taagaagcaa attaggtatt aaagatctgc
     7981 gatatcatga tttgcgtaga gaaggggcaa gtcgcttatt tgaggctggt tttagtattg
     8041 aggaagtcgc tcaagttaca gggcatcgtt cattaaacgt gctatggcag gtatataccg
     8101 aactgtatcc gaaatcttta cataatcgtt ttgaagaact ccaaaagagc agaaacaaga
     8161 cctcttgaca ctgtttattt atacagataa aaataacact gtatacaaac acagtataga
     8221 gggattttta tgcgtattga aatctgcata gccaaagaga aaatgactaa aatgccaaac
     8281 ggtgctgtgg atgcgttaaa ggaagaatta actcggcgca tcagtaagcg ttatgacgat
     8341 gtagaagtga tcgtaaaagc caccagcaat gatgggcttt ctgttacacg cactgcagat
     8401 aaggattctg caaaaacatt tgttcaggag actctgaaag atacctggaa gtctgctgac
     8461 gagtggtttg ttcactaatt agcacgtaaa tcggtaacgg ctggaaatca tccaagacta
     8521 gcactatcga aagttcacca gccaaccgca gtacgttctt gcatacgagg tgccgcgttt
     8581 tacatgccgt accgagaatt aatttcattt tacgaaaagt taacccgcca ttgctacctt
     8641 aattttttaa aacgaagtta cctctgtcag ggtcaaaagc acaattatct cttcctacaa
     8701 tcattgaaac tgcgattgat tccctgctca aaggatgagc cgcccttgtt ttaacaagat
     8761 gtattaatgc cattttatca tacaacatac aaacatttga attcattgaa ttcctcataa
     8821 ataccccatc ttctggttca cataatgtaa cagggcatgt tcgtaagctc tctggcactt
     8881 gcaattgtgc atgctccaca ttaaatgcac atacttcaat tttatctaac aactcctcta
     8941 actggtagtt agaaagcaaa tccatatttt cgagcatctc aatttcacgc agtactgtaa
     9001 cttcacggct tgtcaaacta ccatgattaa gtgtacgttc aatatgttct atatattgaa
     9061 ggccaatctg gatattatca ttcgataaac catcctggtt aagac
//
LOCUS       DAZKFZ010000188         8808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000188 DAZKFZ010000000
VERSION     DAZKFZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1386
                     /locus_tag="ACHOQW_003814"
     CDS             <1..1386
                     /locus_tag="ACHOQW_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFP9574811.1"
                     /translation="RIAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYPPDTIRV
                     GTGDRMRFTKSDRGRGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPAQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGRTEQA
                     VREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVR
                     DLQKEKTLGGD"
     gene            1406..2152
                     /gene="traX"
                     /locus_tag="ACHOQW_003815"
     CDS             1406..2152
                     /gene="traX"
                     /locus_tag="ACHOQW_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP9574812.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            2211..3071
                     /locus_tag="ACHOQW_003816"
     CDS             2211..3071
                     /locus_tag="ACHOQW_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9574813.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            3174..3734
                     /locus_tag="ACHOQW_003817"
     CDS             3174..3734
                     /locus_tag="ACHOQW_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP9574814.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3867..4079
                     /locus_tag="ACHOQW_003818"
     CDS             3867..4079
                     /locus_tag="ACHOQW_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP9574815.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            4324..4785
                     /locus_tag="ACHOQW_003819"
     CDS             4324..4785
                     /locus_tag="ACHOQW_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP9574816.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            4831..5040
                     /gene="hha"
                     /locus_tag="ACHOQW_003820"
     CDS             4831..5040
                     /gene="hha"
                     /locus_tag="ACHOQW_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP9574817.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            5078..5416
                     /locus_tag="ACHOQW_003821"
     CDS             5078..5416
                     /locus_tag="ACHOQW_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574818.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            5656..5910
                     /locus_tag="ACHOQW_003822"
     CDS             5656..5910
                     /locus_tag="ACHOQW_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP9574819.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            6146..6220
                     /gene="tap"
                     /locus_tag="ACHOQW_003823"
     CDS             6146..6220
                     /gene="tap"
                     /locus_tag="ACHOQW_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP9574820.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            6213..7070
                     /gene="repA"
                     /locus_tag="ACHOQW_003824"
     CDS             6213..7070
                     /gene="repA"
                     /locus_tag="ACHOQW_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP9574821.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            7983..8267
                     /locus_tag="ACHOQW_003825"
     CDS             7983..8267
                     /locus_tag="ACHOQW_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP9574822.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            8267..8542
                     /locus_tag="ACHOQW_003826"
     CDS             8267..8542
                     /locus_tag="ACHOQW_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9574823.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            8637..>8808
                     /locus_tag="ACHOQW_003827"
     CDS             8637..>8808
                     /locus_tag="ACHOQW_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574824.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHK"
BASE COUNT         2285 a         2056 c         2476 g         1991 t
ORIGIN      
        1 cggattgccg gtatcagcag ggatgacggg ctgataactc tgcaggatgc ggaaggtaac
       61 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacactgta ccccccggac
      121 accatcaggg tggggaccgg tgaccggatg cgcttcacga agagtgaccg ggggcgtggt
      181 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacgctgtcg
      241 ggcggacagc agacccgggt gattcgcccc gcccaggagc gggcagagca acatattgac
      301 ctggcctatg ccatcaccgc tcacggtgcg cagggggcaa gtgaaacctt tgccatcgcg
      361 cttgaaggta cggaaggcag ccggaaactg atggccggct ttgagtcagc ctacgtggcc
      421 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
      481 gccattaaca atgccgtaca gaaaggaaca gcccacgatg tgtttgaacc gaaaccggac
      541 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
      601 gcagggcgtg ccgttcttcg tcaggcgggg cttgccgggg gagacagtcc tgcacggttt
      661 attgctccgg gacgtaaata tccacagccg tatgtggcac tgccggcgtt tgaccgtaac
      721 ggcaagtctg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
      781 ggattcagtg gtgaaggacg ggtgaaaggc agcggggatg cgcagttcgt ggccctgcag
      841 ggcagccgta acggagagag cctgctggct gataatatgc aggatggtgt ccggattgcc
      901 cgtgataatc ctgacagcgg tgtggtggtg agaatcgccg gtgaaggtcg tccgtggaat
      961 cccggtgcca tcaccggtgg tcgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     1021 ggagccggaa atggcgagcc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     1081 gccatccgcc gtgaaacgga acgccgcgca gatgaaattg tccgtaaaat ggcagagaac
     1141 aaacctgacc tgccggacgg cagaacagag caggctgtca gggagattgc cgggcaggag
     1201 cgtgaacggg ctgtcacttc tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     1261 ccacaacggg agcgggaggc ggtccgtgag gttgtccggg aaaacctgct gcaggagcga
     1321 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cctgggcgga
     1381 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     1441 ttcactggct gcccggaccg atacctggtt gcagtcgttg ctggtctggt cacccggaca
     1501 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccacg caaaccgcat
     1561 tctgcatctg gaccagacgt ggatgtttct ggccgggcgt ggagcctttc cgttgtttgc
     1621 tctggtgtgg gggctgaatc tgtcccgcca tgcgcatatc aggcagtcgg ccattaaccg
     1681 gctgtgggga tggggaatta ttgcccagtt cgcgtattat ctggcaggct ttccgtggta
     1741 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     1801 gcgcagtggg tggcgtacag cagcggccat tctcctgatg gcactgtggg ggcctttgtc
     1861 cggcaccagt tacggcattg ccgggctgct gatgctggca gtcagccacc ggctgtaccg
     1921 ggcggaagac agaacggaac gtctggtgct ggttgcatgc ctgctggccg tgattccggc
     1981 acttaacctt gcctccagtg atgcggcggc tgtagctggt cttgtgatga cggtgctgac
     2041 cgttggtctg gtgtcctgtg cagggaaatc attaccccgt ttctggcccg gagatttttt
     2101 cccgacgttc tatggctgtc atctggctgt gctgggcatt ctggcgttgt gactggtatg
     2161 atactgacgc tgattctgct tattaataaa caacataaca caggatattt atgaaaatta
     2221 cggatcacaa actctctgaa ggtattgccc tgacatttcg tgttcctgaa ggaaatataa
     2281 aacatccttt gattatctta tgccatgggt tttgcggtat caggaatgtt ctgctccctt
     2341 gctttgcgaa tgccttcaca gaagcaggat tcgcaacgat tacttttgat tatcgaggat
     2401 tcggtgagag tgatggagaa cgagggcgtc tggttcctgc aatgcagacc gaagatatta
     2461 tttcagttat caattgggca gaaaagcagg aatgtattga taatcagcgt attgggttat
     2521 ggggaacatc gctgggaggg gggcatgtct tcagtgcagc tgcgcaggac caacgcgtaa
     2581 aatgtattgt cagccagctg gcttttgctg acggtgatgt tctggttacc ggagagatga
     2641 atgaatcgga gagagcgtct tttctatcca cgctgaataa aatggcagag aagaagaaaa
     2701 acaccgggaa agagatgttt gtgggggtta cacgggtact aagtgatgat gaatcgaagg
     2761 tattcttcga gaaaatcaaa gcccggcatc ccgaaatgga tataaaaata cctttcctta
     2821 cggtcatgga aacgttgcaa tataaaccgg cagagagtgc agccagcgtc cagtgcccag
     2881 ttttggttgt tattgccggc caggatagcg ttaatccacc ggaacaggga agagcattat
     2941 atgatgctgt tgcttctggt actaaggaat tatacgagga agctgatgca tgccattatg
     3001 atatttatga gggcgcgttt tttgaacgtg tagtagctgt gcagacacaa tggtttaaac
     3061 aatatcttta attgtcagta tttatacgtt aaaggtcgtt gaaattaact tcctgttacg
     3121 atgagccgtt tcaacggcct gcagggcata ttttcacgaa ggtcagagta agcatggcag
     3181 agcaaaaacg accggtactg acactgaagc ggaaaacgga aggagagacg cctgtccgca
     3241 gccggaaaac catcatcaat gtcaccacgc caccaaaatg gaaggtgaaa aagcagaagc
     3301 tggccgagaa agcagcccgg gaagcggagc tggcggcaaa aaaagcgcag gccagacagg
     3361 cgctgtccat ttatctgaac ctgcccacgc aggatgaggc cgtgaacacc ctgaaaccct
     3421 ggtggccggg attatttgac ggtgacacgc cccggcttct ggcctgcggt atccgggacg
     3481 tgttactgga agacgtggcg cagcggaata tcccgctctc gcataaaaag ctgcgcaggg
     3541 cgctgaaggc catcacccgt tcagaaagct atctgtgtgc catgaaagcc ggtgcctgcc
     3601 ggtatgacac ggaagggtat gtgacggagc atatttctca ggaggaggaa gcgtatgcgg
     3661 cagcgcgtct ggataaaatc cgccgccaga accggataaa ggcagaactt caggccgtgc
     3721 ttgatgagaa ataaaaaaag cctccctcag agagaggagg cagggaaata aggcttaaaa
     3781 ggaaataact tccgtacccg aaaggaagcc aattcagtag attatttaac accataatac
     3841 tgacgggtta aagaggagtg ataaagatga atggattcag aaacagttca cggaacggtc
     3901 aggtctggcg ttaccagcgt gccggaagcc gggccgttat tctggaagtc agtggacgct
     3961 ggatggaagc ggcagaagca tggcgacggg ctgccggtgt tgcaccccgg actgactggc
     4021 agcagtttgc ccgaaaaagg gctgaacact gccaccggcg ctgccgagga agggggtaac
     4081 gaaaaaagca gccttcatta ctccagggat tcagaaggct gaataatgca tgagatttgc
     4141 tttttttaat atccggtcgg gcagtatgac cggccagttc tgagtagcac agagtttttt
     4201 ccgtattaag tcgtgatttt atattttgtg attaatttca caaaataagg tgttattaag
     4261 tgtgtgctgc aatattcagg atgcctgaaa tacaggtgct atttatctga tacggagaat
     4321 aacatgagga aatatattcc actggtatta tttatctttt catggccggt attgtgtgca
     4381 gatattcatg gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca
     4441 cggaaggcgg tgcggatcag gctggtaaat attgatgcgc cggaaaagaa acaggattat
     4501 ggccggtggt cgacagacat gatgaaatca ctggttgcag gaaaaacggt taccgtcaca
     4561 tattttcagc gagatcgtta tggtcgtatg ctcgggcagg tttatgcacc agacggaatg
     4621 aatgttaatc agtttatggt ccgggccggg gccgcctggg tatatgagca gtacaacaca
     4681 gatcccgtgc tgcctgtcct tcagaacgaa gccagacaac aaaaacgtgg gctctggtca
     4741 gatgctgatc cggttccccc ctggatatgg agacaccgta aataaagcga tggcaatctt
     4801 atcattcatc tgttctcagc gggaataaaa atggaaaaaa caaaacagga atggctgtat
     4861 caattacggc gatgttcatc agtcaatacg ctggaaaaaa ttattcataa aaacagagac
     4921 agtttatcga ccagtgaacg tgaatcattt aattcagcag cagaccaccg gttagcagaa
     4981 ttaatcaccg gaaagttata tgacaggata ccaaaagaaa tatggaaata tgtaaggtaa
     5041 tagttcagca taattaatta ccaggagcag ataaacaatg aagctgatta tttttattct
     5101 cattgttctg attattgcag cgttacttat ccggattatc ctcagaagtg taaatcagca
     5161 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg accggtgatg cagagcgaat
     5221 actgtccggc ggtgaatatg atgccagcca tttacgacct gaacgcagac gccgggatat
     5281 tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc acgatgccag
     5341 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgctc agcagtcacc
     5401 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
     5461 gcaagttttc cccgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
     5521 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgtcacaa
     5581 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttaag
     5641 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatc tggcgcaaaa
     5701 cgagcataca gaaaggggaa tccgctttct gatgcagaga aacaaagatt atcagtggcc
     5761 cgtaaaagag cttcgttcaa ggaagtaaaa gtatttcttg aaccaaagta taaggccatg
     5821 ctcatgcaaa tgtgtcatga agatggcctg actcaggctg aagttctgac cgcactgata
     5881 aaaagtgaag cgcaaaaacg atgtgtgtga tgatgggctt acattcttga gtgttcagaa
     5941 gattagtgct agattactga tcgtttaagg aattttgtgg ctggccacgc cgtaaggtgg
     6001 cagggaactg gttctgatgt ggatttacag gagccagaaa agcaaaaacc ccgataatct
     6061 tcagaaagtt tggcgactga gaagattacc ggggcccact taaaccgtat agccaacaat
     6121 tcagctatgc ggggagtata gttatatgct cggaaaagtt caagacttct ttctgtgctc
     6181 gctccttctg cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acgtattacc
     6241 gccaggtaaa gaacccgaat ccggtgttca caccccgtga aggtgccgga acgctgaagt
     6301 tctgcgaaaa actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc
     6361 atgtggcaca tgcccgttcg aagggactgc gtcggcgcat gccaccggta ctgcgtcgcc
     6421 gggctattga tgcgctgctg caggggctgt gttttcacta tgacccgctg gccaaccgtg
     6481 tccagtgctc catcaccaca ctggccattg agtgcggact ggcgacggag tctgctgccg
     6541 gaaaactctc catcacccgt gccacccggg ccctgacgtt cctgtcagag ctgggactga
     6601 ttacctacca gacggaatat gacccgctta tcgggtgcta cattccgacc gacatcacgt
     6661 tcacaccggc tctgtttgct gcccttgatg tgtctgagga tgcagtggca gctgcgcgcc
     6721 gcagccgtgt tgaatgggaa aacaggctgc gcaaaaagca ggggctggat accctgggta
     6781 tggatgaact gatagcgaaa gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga
     6841 cagagcttaa gtcacgggga ataaagcgtg cccgtgcgcg tcgtgatgcg ggcagggaac
     6901 gtcaggatat cgtcaccctg gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct
     6961 tcactgctaa tcgtgaggcg gtaaaacgcg aagtggagcg tcgtgtgaag gagcgcatga
     7021 ttctgtcacg taaccgcaat tacagccggc tggccacagc ttccccctga aagtgacctc
     7081 ctcagaataa tccggcacgc gccggaggca ttcgcacgcc tgaagcccgc cggtgcacaa
     7141 aaaatcagca ccacatccca aaaacaacct catcatctac cttctggtgc atccggtccc
     7201 ccctgttttg atacaaaaca cgtctcacag acgggcaatt ttgtttatcc acattaaact
     7261 gcaagggatt tctctgtaag attacaaccg ttcatgtcat aaagcaccag ccgccaggct
     7321 tacagggtgc aatgtatctt ttaaacaccc gtttatatct cctttaaact acttaattac
     7381 attcatttaa aaaaaagact tattcactgc ctgtcctgtg gacagacaga tatgcacctc
     7441 ccaccgcaag cggcgggccc caaccggagc cactttagtt acaacacaca aaaacaacct
     7501 ccagaaaaac cccggtcccg ctcagaactg aaaccacaaa gcccctccct cataactgaa
     7561 aaacggcccc gccccggccc aaagggccgg aacagagtcg cttttaatta tgaatgttgt
     7621 aactaagcat catcatcgct gtcagtcttc tggctgaacg ttctcagtac acgctcgtaa
     7681 gcggccctga gggctcgcta acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg
     7741 gaccactccg accgcgcaca taaactctct catggctgaa agcgggtatg gtctggcagg
     7801 gctggggggg gggtaaggtg aaatccatca atcagtaccg gcttacgccg ggcttcggcg
     7861 gttttacacc tgtgctacat gtaacctccg tataccgcct tacatgcgct gacgcgcatt
     7921 atttgccttg ttccgtctga aagtaatcac tatgattaaa tatgattaac gactaatcgg
     7981 atatgcaaat gaaaaacaat accgcacaag caacaaaagt cattaccgcg catgtgccat
     8041 tacctatggc tgataaagtc gaccagatgg ccgccagact ggaacgctcc cggggttggg
     8101 ttatcaaaca ggcgctttct gcatggcttg cccaggagga ggagcgtaat cgcctgacgc
     8161 tggaagccct ggacgatgtg acatccggac aggttattga ccatcaggct gtacaggcct
     8221 gggcggacag cctcagtact gacaatccgt taccggtgcc acgctgatgg aactgaagtg
     8281 gagcagtaag gcgctttctg atttggcgcg gttatatgat tttctggtgc tagccagtaa
     8341 acctgctgcc gccagaacgg tacagtccct gacacaggca ccggtcattc tgttaactca
     8401 tccacgtatg ggggaacagt tatttcagtt tgaacccagg gaggtcagac ggatttttgc
     8461 tggcgagtac gaaatccgtt acgaacttaa tggccagact atttatgtat tgcgcctgtg
     8521 gcacacacga gaaaacaggt agattgtgtg tggggacaac ttaacatagc ctgcagatat
     8581 taattctgga acattattat caggccagat atttaaataa tcttgtagga gataaaatga
     8641 attacgatga aataacgaaa attaccgctg aacgtattag cgactacatg actgaagcag
     8701 ttaatacaga ctctatagcc gttgcggaaa tgtttcataa cgcagcatgg ggagtgcgaa
     8761 cattgtggtt tgaactcgtc acaaaaatag atatagatat acataaaa
//
LOCUS       DAZKFZ010000189         8699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000189 DAZKFZ010000000
VERSION     DAZKFZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(325..588)
                     /gene="rpsT"
                     /locus_tag="ACHOQW_003828"
     CDS             complement(325..588)
                     /gene="rpsT"
                     /locus_tag="ACHOQW_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9574825.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            691..915
                     /locus_tag="ACHOQW_003829"
     CDS             691..915
                     /locus_tag="ACHOQW_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP9574826.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            923..1864
                     /gene="ribF"
                     /locus_tag="ACHOQW_003830"
     CDS             923..1864
                     /gene="ribF"
                     /locus_tag="ACHOQW_003830"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP9574827.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1907..4723
                     /gene="ileS"
                     /locus_tag="ACHOQW_003831"
     CDS             1907..4723
                     /gene="ileS"
                     /locus_tag="ACHOQW_003831"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9574828.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4723..5217
                     /gene="lspA"
                     /locus_tag="ACHOQW_003832"
     CDS             4723..5217
                     /gene="lspA"
                     /locus_tag="ACHOQW_003832"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9574829.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5305..5754
                     /gene="fkpB"
                     /locus_tag="ACHOQW_003833"
     CDS             5305..5754
                     /gene="fkpB"
                     /locus_tag="ACHOQW_003833"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9574830.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5756..6706
                     /gene="ispH"
                     /locus_tag="ACHOQW_003834"
                     /gene_synonym="lytB"
     CDS             5756..6706
                     /gene="ispH"
                     /locus_tag="ACHOQW_003834"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP9574831.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6772..7686
                     /gene="rihC"
                     /locus_tag="ACHOQW_003835"
     CDS             6772..7686
                     /gene="rihC"
                     /locus_tag="ACHOQW_003835"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP9574832.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7853..8674
                     /gene="dapB"
                     /locus_tag="ACHOQW_003836"
     CDS             7853..8674
                     /gene="dapB"
                     /locus_tag="ACHOQW_003836"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9574833.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
BASE COUNT         1995 a         2169 c         2461 g         2074 t
ORIGIN      
        1 taatgaggag atatttatga atggttaaag tatataggaa tttaattcaa aaatagagtg
       61 gtcttaagtt taatacctca atgtgtatca caatatccac attctttgta ggggagtctg
      121 gagattgagt agatattctt gttcagaatg tatcagccga tggttctacg attcttaagc
      181 cacgaagagt tcagatagtg caacggcatg tctcttttga ctatctggca accggcagtg
      241 tgttctctca cgcatcacaa aagcagcagg cataaaaaaa cccgcttgcg cgggcttttt
      301 cacaaagctt cagcaaattg gcaattaagc cagtttgttg atctgtgcag tcaggttagc
      361 cttatgacgt gcagctttgt ttttgtggat cagaccttta gcagcctgac ggtccacgat
      421 cggttgcatt tcgttaaatg ctttctgtgc agcagctttg tcgccagctt cgatagctgc
      481 gtatactttc ttgatgaaag tacgcatcat agagcgacgg cttgcgttgt gcttacgagc
      541 cttttcagac tgaatggcgc gcttcttagc tgatttgata ttagccaagg tccaactccc
      601 aaatgtgttc tatatggaca attcaaaggc cgaggaatat gcccttttag ccttcttttg
      661 tcaatggatt tgtgcaaata agcgccgtta atgtgccggc actcgttacg tagtgatggc
      721 gcaggattct accagcttgc ggggtgtgaa tacagctttt ccgcgataaa aattgcagca
      781 ggcagtcagt ttcttcccgt gatttgcgcc atgaccatga caatgaaaag ccacttcttt
      841 ctgatttcgg tactcaatcg ccggttaacc ttgaccgctg tacaaggtat actcggacga
      901 ttttcactgt tttgagccag acatgaagct gatacgcggc atacataatc tcagccaggc
      961 cccgcaagaa gggtgtgtgc tgactattgg taatttcgac ggcgtgcatc gcggtcatcg
     1021 cgcgctgtta cagggcttgc aggaagaagg gcgcaagcgc aacttaccgg tgatggtgat
     1081 gctttttgaa cctcaaccac tggaactgtt tgctaccgat aaagccccgg cacggctgac
     1141 ccgactgcgg gaaaaactac gttaccttgc agagtgtggc gttgattacg tgctgtgcgt
     1201 acgtttcgac aggcgtttcg cggcgttaac cgcgcaaaat ttcatcagcg atcttctggt
     1261 gaagcatttg cgggtaaaat tcctcgccgt gggggatgat ttccgctttg gcgctggtcg
     1321 ggaaggcgat ttcttgttat tacagaaagc tggcatggaa tacggcttcg atatcaccag
     1381 tacgcaaact ttttgcgaag gtggcgtgcg tatcagcagc accgccgtgc gtcaggccct
     1441 tgcggatgac aatttggctc tggcagaaag tttactgggg cacccgtttg ccatctccgg
     1501 gcgtgtagtc cacggtgatg aattagggcg cactataggt ttcccgacgg cgaatgtacc
     1561 gctacgccgt caggtttccc cggtgaaagg ggtttatgcg gtagaagtgc tgggcctcgg
     1621 tgaaaagccg ttacccggcg tggcaaacat cggaacacgc ccaacggttg ccggtattcg
     1681 ccagcagctg gaagtgcatt tgttagatgt tgcaatggac ctttacggtc gccatataca
     1741 agtagtgctg cgtaaaaaaa tacgcaatga gcagcgattt gcttcgctgg acgaactgaa
     1801 agcgcagatt gcgcgtgatg aattaaccgc ccgcgaattt tttgggctaa caaaaccggc
     1861 ttaagcctgt tatgtaatca aaccgaaaca cggaaccgag aatctgatga gtgactataa
     1921 atcaaccctg aatttgccgg aaacagggtt cccgatgcgt ggcgatctcg ccaagcgcga
     1981 accgggaatg ctggcgcgtt ggactgatga tgatctgtac ggcatcattc gtgcggctaa
     2041 aaaaggcaaa aaaaccttca ttctgcatga tggccctcct tatgcgaatg gcagcattca
     2101 tattggtcac tcggttaaca agattctgaa agacattatc gtgaagtcca aagggctttc
     2161 cggttatgac tcgccgtatg tgcctggctg ggactgccac ggtctgccga tcgagctgaa
     2221 agtagagcaa gaatacggta agccggatga gaaattcacc gccgccgagt tccgcgccaa
     2281 gtgccgcgaa tacgcggcga cccaggttga cggtcaacgc aaagacttta tccgtctggg
     2341 cgtgctgggc gactggtcgc acccgtacct gaccatggac ttcaaaactg aagccaatat
     2401 catccgcgcg ctgggcaaaa tcatcggcaa tggtcacctg cacaaaggcg cgaagccggt
     2461 gcactggtgc gtagactgcc gttctgcact ggcagaagcg gaagttgagt attacgacaa
     2521 aacttctccg tccattgacg tcgctttcca ggcggtcgat caggatgcgc tgaaagcgaa
     2581 atttggcgta agcaacgtta acggcccaat ctcgctggtg atctggacca ctacgccgtg
     2641 gactctgcct gcgaaccgcg caatctctat tgcacctgat ttcgactatg cgctggtgca
     2701 gatcgacggt caggccgtga ttctggcgaa agatctggtt gaaagcgtaa tgcagcgtat
     2761 cggcgtgacc gattacacca ttctcggcac ggtaaaaggt gcggagcttg agctgctgcg
     2821 ctttgcccat ccgtttatgg gcttcgacgt cccggcaatc ctcggcgatc acgttaccct
     2881 ggatgccggt accggtgccg ttcacaccgc gcctggccac ggcccggacg actatgtgat
     2941 cggtcagaaa tacggcctgg aaaccgctaa cccggttggc ccggacggca cttatctgcc
     3001 gggcacttat ccgacgctgg atggcgtgaa cgtcttcaaa gcgaacgaca tcgtcgttgc
     3061 gctgctgcag gaaaaaggcg cgctgctgca cgttgagaaa atgcagcaca gctatccgtg
     3121 ctgctggcgt cacaaaacgc cgatcatctt ccgcgcaacg ccgcagtggt tcgtcagtat
     3181 ggatcagaaa ggtctgcgcg cgcagtctct gaaagagatc aaaggtgtgc agtggatccc
     3241 ggactggggc caggcgcgta tcgagtcgat ggtcgctaac cgtcctgact ggtgtatctc
     3301 ccgtcagcgt acctggggcg taccgatgtc tctgttcgtg cacaaagaca cggaagagct
     3361 gcatccgcgt accctcgaac tgatggaaga agtggctaaa cgcgttgaag ttgatggcat
     3421 ccaggcgtgg tgggatcttg atgcgaaaga gatcctcggc gatgaagctg atcagtatgt
     3481 gaaagtgccg gatacgctgg atgtatggtt tgactccggc tctactcact cttctgttgt
     3541 tgacgtgcgc ccggaatttg ccggtcacgc tgcggacatg tatctggaag gttctgacca
     3601 acaccgtggc tggttcatgt cttctctgat gatctccacc gcgatgaagg gcaaagcgcc
     3661 gtatcgtcag gtgctgaccc acggctttac cgtagatggt cagggccgca agatgtctaa
     3721 atccatcggc aacaccgtgt cgccgcagga tgtgatgaac aaactgggcg cggatattct
     3781 gcgtctgtgg gtggcatcaa ccgactacac cggtgaaatg gccgtttctg acgagatcct
     3841 gaaacgtgct gccgacagct atcgtcgtat ccgtaacacc gcgcgcttcc tgctggcaaa
     3901 cctgaacggt tttgatccgg caaaagatat ggtgaaaccg gaagagatgg tagtactgga
     3961 tcgctgggcc gtaggttgtg cgaaagcggc acaggaagac atcctcaagg catacgaagc
     4021 atacgatttc cacgaagtgg tacagcgtct gatgcgcttc tgctccgttg agatgggttc
     4081 cttctacctc gacatcatca aagaccgtca gtacaccgcc aaagcggaca gcgtggcgcg
     4141 tcgtagctgc cagactgcgc tgtatcacat cgcagaagcg ctggttcgct ggatggcacc
     4201 aatcctctcc ttcaccgctg atgaagtgtg gggctacctg ccgggcgaac gtgaaaaata
     4261 cgtcttcacc ggcgagtggt acgaaggtct gtttggtctg gcagacagtg aagcaatgaa
     4321 cgatgcgttc tgggacgagc tgttgaaagt gcgtggcgaa gtgaacaaag tcattgagca
     4381 ggcgcgtgcc gacaagaaag tgggtggctc gctggaagcg gcagtaacct tgtatacaga
     4441 accggaactg gcggcgaaac tgaccgcatt gggcgatgaa ttacgatttg tcctgttgac
     4501 ctccggcgct accgttgcag actataacga cgcacctgct gatgctcagc agagcgaagt
     4561 gctcaaaggg ctgaaagtcg cgttgagtaa agccgaaggt gagaagtgcc cacgctgctg
     4621 gcactacacc caggatgtcg gcaaggtggc ggaacacgca gaaatctgcg gccgctgtgt
     4681 cagcaacgtc gccggtgacg gtgaaaaacg taagtttgcc tgatgagtca atcgatctgt
     4741 tcaacagggc tacgctggct gtggctggtg gtagtcgtgc tgattatcga tctgggcagc
     4801 aaatacctga tcctccagaa ctttgctctg ggggatacgg tcccgctgtt cccgtcgctt
     4861 aatctgcatt atgcgcgtaa ctatggcgcg gcgtttagtt tccttgccga tagcggcggc
     4921 tggcagcgtt ggttctttgc cggtattgcg attggtatta gcgttaccct ggttgtgatg
     4981 atgtatcgct cgaaggccac gcagaagcta aacaatatcg cctacgcgct gattattggc
     5041 ggcgcgctgg gcaacctgtt cgaccgcctg tggcacggct tcgttgtcga tatgatcgac
     5101 ttctacgtcg gcgactggca cttcgccacc ttcaacctcg ccgatactgc catctgtgtt
     5161 ggtgcggcac tgattgtgct ggaaggtttt ttgccttcta aagcgaaaaa acaataataa
     5221 accctgccgg ataaggccta cagattgctg cgaaatcgta ggccgcatcc ggcaaaaatc
     5281 ctcaaaatat aagagcaaac ctgcatgtct gaatctgtac agagcaatag cgccgtcctg
     5341 gtgcacttca cgctaaaact cgacgatggc accaccgccg agtctacccg caacaacggt
     5401 aaaccggcgc tgttccgcct gggcgatgct tctctttccg aagggctgga gcaacacctg
     5461 ctggggctga aagtgggcga taaaaccact ttctcgctgg agccagatgc ggcgtttggc
     5521 gtgccgtcac cggacctgat tcagtacttc tcccgccgtg agtttatgga tgcaggcgag
     5581 ccagaaattg gcgcaataat gctttttacc gcaatggatg gcagtgagat gcctggcgtg
     5641 atccgcgaaa ttaacggcga ctccattacc gtcgatttca accatccgct ggccgggcag
     5701 accgttcatt ttgatattga agtgctggaa atcgatccgg cactggaggc gtaacatgca
     5761 gatcctgttg gccaacccac gtggtttttg tgccggggta gaccgcgcta tcagcattgt
     5821 tgaaaacgcg cttgccattt acggcgcacc gatatatgtc cgtcacgaag tggtgcataa
     5881 ccgctacgtg gtcgatagcc tgcgcgagcg tggagctatc tttattgagc agatcagcga
     5941 agtgccggac ggcgcgatcc tgatcttctc cgcacatggt gtttctcagg cggtacgtaa
     6001 cgaagcgaaa agccgtgatt tgacggtatt cgacgccacc tgtccgctgg tgaccaaagt
     6061 gcatatggaa gtcgcccgcg ccagccgtcg tggcgaagag tctattctca tcggtcacgc
     6121 cgggcacccg gaagtggaag ggacgatggg gcagtacagc aaccctgaag ggggaatgta
     6181 tctggtcgaa tcgcctgacg atgtgtggaa actgacggtc aaaaacgaag agaagctctc
     6241 ctttatgacc caaaccacgc tgtcggtaga tgacacgtct gatgtgatcg acgcgctgcg
     6301 taaacgcttc ccgaaaattg tcggtccgcg caaagatgac atctgctacg ccacgactaa
     6361 ccgtcaggaa gcggtacgcg ccctggcaga acaggcggaa gttgtgttgg tggtcggttc
     6421 gaaaaactcc tccaactcca accgtctggc ggagctggcc cagcgtatgg gcaaacgcgc
     6481 gtttttgatt gacgatgcga aagatatcca ggaagagtgg gtgaaagagg ttaaatgcgt
     6541 cggcgtgact gcgggcgcat cggctccgga tattctggtg cagaatgtgg tggcacgttt
     6601 gcagcagctg ggtggtggtg aagccattcc gctggaaggc cgtgaagaaa atattgtttt
     6661 cgaagtgccg aaagagctgc gtgtcgatat tcgtgaagtc gattaagtca ttagcagcct
     6721 aagttatgcg aaaatgccgg tcttgttatc ggcatttttt atggagaaaa catgcgctta
     6781 cctatcttcc tcgatactga ccccggcatt gacgatgccg tcgccattgc cgccgcgatt
     6841 tttgcccccg aactcgacct gcaactgatg accaccgtcg cgggtaatgt ctcggttgag
     6901 aaaactaccc gtaacgcctt gcaactgctg catttctgga atgcggagat tccgctcgcc
     6961 cagggagccg ctgtgccgct ggtacgcgca ccgcgtgatg cagcatctgt gcacggcgaa
     7021 tcgggaatgg ctggctacga ctttgttgag cacaaccgaa agccactcgg gataccggcg
     7081 tttctggcga ttcgggatgc cctgatgcgt gcaccagagc ccgttaccct ggtggccatc
     7141 ggcccgttaa ccaatattgc gctgttactt tcacaatgcc cggaatgtaa accgtatatt
     7201 cgccgtctgg tgatcatggg tggttctgcc ggacgcggca actgtacgcc aaacgccgag
     7261 tttaatattg ctgccgatcc agaagctgct gcctgtgtct tccgcagtgg tattgaaatc
     7321 gtcatgtgcg gtttggatgt caccaatcag gcaatattaa ctcctgacta tctctctaca
     7381 ctgccgcagt taaaccgtac cgggaaaatg cttcacgccc tgtttagcca ctaccgtagc
     7441 ggcagtatgc aaagcggctt gcgaatgcac gatctctgcg ccatcgcctg gctggtgcgc
     7501 ccggacctgt tcactctcaa accctgtttt gtggcagtgg aaactcaagg cgaatttacc
     7561 tcaggcacga cggtggttga tatcgacggt tgcctgggca aaccagccaa tgtacaggtg
     7621 gcattggatc tgaatgtaaa aggcttccaa cagtgggtgg ctgaggtgct ggctctggtg
     7681 ccgtaacctg tcacatgtta tcggcatgca gtcattcatc gactcatgcc tttcactgat
     7741 atccctccct gtttatcatt aatttctaat tatcagcgtt tttggctggc ggcgtagcga
     7801 tgcgctggtt actctgaaaa cggtctatgc aaattaacaa aagagaatag ctatgcatga
     7861 tgcaaacatc cgcgttgcca tcgcgggagc cggggggcgt atgggccgcc agttgattca
     7921 ggcggcactg gcattagaag gcgtgcagct gggcgcggcg ctggagcgcg aaggatcttc
     7981 tttactgggc agcgacgccg gtgagctggc tggtgcaggg aaaacaggcg ttaccgtgca
     8041 aagcagtctt gatgcgataa aagatgattt tgatgtgttt atcgatttta cccgtccgga
     8101 aggtacgctg aaccatctcg ctttttgtcg ccagcatggc aaagggatgg tgatcggcac
     8161 tacggggttt gacgaagccg gaaaacaggc aattcgtgac gccgccgccg atattgcgat
     8221 tgtctttgcc gccaatttta gcgttggcgt taacgtcatg cttaagctgc tggagaaagc
     8281 agccaaagtg atgggtgact acaccgatat tgaaattatt gaagcacatc atagacataa
     8341 agttgatgcg ccgtcaggca ccgcactggc aatgggagag gcgatcgccc acgcccttga
     8401 taaagatctg aaagattgcg cggtctacag tcgtgaaggc cataccggtg aacgtgtgcc
     8461 tggtaccatt ggttttgcca ccgtgcgtgc aggtgacatc gttggcgaac ataccgcgat
     8521 gtttgccgat attggcgagc gtctggagat cacccataag gcgtccagcc gcatgacatt
     8581 tgctaacggt gcggtaagat cggctttgtg gttgagtggt aaggaaaatg gtctttttga
     8641 tatgcgagat gtgcttgatc tcaataattt gtaaccacaa aatgtttatg atgatgcaa
//
LOCUS       DAZKFZ010000190         8659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000190 DAZKFZ010000000
VERSION     DAZKFZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..1226
                     /gene="lacY"
                     /locus_tag="ACHOQW_003837"
     CDS             3..1226
                     /gene="lacY"
                     /locus_tag="ACHOQW_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP9574834.1"
                     /translation="MFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISL
                     FSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGI
                     YLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVF
                     WLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLY
                     VIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRI
                     GGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVR
                     FSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSG
                     PGPLSLLRRQVNEVA"
     gene            1292..1903
                     /gene="lacA"
                     /locus_tag="ACHOQW_003838"
     CDS             1292..1903
                     /gene="lacA"
                     /locus_tag="ACHOQW_003838"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP9574835.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(2006..3187)
                     /gene="cynX"
                     /locus_tag="ACHOQW_003839"
     CDS             complement(2006..3187)
                     /gene="cynX"
                     /locus_tag="ACHOQW_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP9574836.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(3193..3663)
                     /gene="cynS"
                     /locus_tag="ACHOQW_003840"
     CDS             complement(3193..3663)
                     /gene="cynS"
                     /locus_tag="ACHOQW_003840"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP9574837.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKALSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(3694..4353)
                     /gene="cynT"
                     /locus_tag="ACHOQW_003841"
     CDS             complement(3694..4353)
                     /gene="cynT"
                     /locus_tag="ACHOQW_003841"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP9574838.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4463..5362
                     /gene="cynR"
                     /locus_tag="ACHOQW_003842"
     CDS             4463..5362
                     /gene="cynR"
                     /locus_tag="ACHOQW_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP9574839.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(5495..6778)
                     /gene="codA"
                     /locus_tag="ACHOQW_003843"
     CDS             complement(5495..6778)
                     /gene="codA"
                     /locus_tag="ACHOQW_003843"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP9574840.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(6768..8027)
                     /gene="codB"
                     /locus_tag="ACHOQW_003844"
     CDS             complement(6768..8027)
                     /gene="codB"
                     /locus_tag="ACHOQW_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP9574841.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         2106 a         2310 c         2247 g         1996 t
ORIGIN      
        1 ggatgttcgg tttattcttt ttcttttact tttttatcat gggagcctac ttcccgtttt
       61 tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacgggt attatttttg
      121 ctgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt tctgacaaac
      181 tcgggctgcg caaatacctg ctgtggatta ttaccggcat gttagtgatg tttgcgccgt
      241 tctttatttt tatcttcggg ccactgttac aatacaacat tttagtagga tcgattgttg
      301 gtggtattta tcttggcttt tgttttaacg ccggtgcgcc cgcagtagag gcatttatcg
      361 agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc gcggatgttt ggctgtgttg
      421 gctgggcgct gtgtgcctcg attgtcggca tcatgttcac catcaataat cagttcgttt
      481 tctggctggg ttctggctgt gcactcatcc tcgccatttt actctttttc gccaaaacgg
      541 atgcgccctc ttccgccacg gttgccaatg cggtaggtgc caaccattcg gcatttagcc
      601 ttaaactggc gctggaactg ttcagacagc caaaactgtg gtttttgtca ctgtatgtta
      661 ttggcgtttc ctgcacctac gatgtttttg accaacagtt tgctaatttc tttacttctt
      721 tctttgccac cggtgaacag ggtacgcggg tatttggcta cgtaacgaca atgggcgaat
      781 tacttaacgc ctcaattatg ttctttgcgc cactgatcat taatcgcatc ggtgggaaaa
      841 atgccctgct gctggctggc actattatgt ctgtacgtat tattggctca tcgttcgcca
      901 cctcagcgct ggaagtggtt attctgaaaa cgctgcatat gtttgaagta ccgttcctgc
      961 tggtgggctg ctttaaatat attaccagcc agtttgaagt gcgtttttca gcgacgattt
     1021 atctggtctg tttctgcttc tttaagcaac tggcgatgat ttttatgtct gtactagcgg
     1081 gtaatatgta tgaaagcatc ggtttccagg gcgcttatct ggtgctgggt ctggtggcgc
     1141 tgggcttcac cttaatttcc gtgttcacgc ttagcggccc cggcccgctt tctctactgc
     1201 gtcgtcaggt gaatgaagtc gcttaagcaa tcaatgtcgg atgcggcgcg agcgccttat
     1261 ccgaccaaca tatcataacg gaatgatcgc aatgaacatg tcgatgacag aaagaataaa
     1321 agcaggcaag ctatttaccg atatgtgcga aggcttacct gaaaaaagac ttcgtagaaa
     1381 aacgttaatg tatgagttta atcactcgca tccatcagaa gttgataaaa gagaaagcct
     1441 gattaaagaa atgtttgcca cggtagggga aaacgcctgg gtagaacccc ccgtctattt
     1501 ctcttacggt tccaacatcc atataggccg caatttttat gcaaatttca atttaaccat
     1561 tgtcgatgac tacacggtaa caatcggtga taacgtattg attgcaccca acgttactct
     1621 ttccgttacg ggacaccctg tacaccatga attgagaaaa aacggtgaga tgtactcttt
     1681 tccgataacg attggcaata acgtctggat cggaagtcat gtggttatta atccaggcgt
     1741 caccattggg gataattctg ttattggcgc gggtagtatc gtcacaaaag acattccacc
     1801 aaacgtcgtg gcggctggcg ttccttgtcg ggttattcgc gaaataaacg accgggataa
     1861 gcaatattat ttcagagatt acaaagttga atcgtcagtt taaattataa aaattgcctg
     1921 atacgcttcg cttatcaggc ctacaaattc agtgatctat gtaggccgca tccggcgtga
     1981 acaaagcgca gaaacaagcg tcgcatcatg cctctttgac ccacagctgc ggaaaacgtg
     2041 ctggtgcaaa acgcagggtt ataatcatca gccctacgac gcacagcgca tgaaacgccc
     2101 agtccatcaa gtaattgccg ctgatactac gcagcacgcc agaaaaccac ggggcaagcc
     2161 cggcgatgat aaaaccgatt ccctgcataa acgccaccag cttgccagca atagccggtt
     2221 gcacagagtg atcgagcgcc agcagcaaac agagcggaaa tgcgccgccc agacctaaac
     2281 cacacaccat cgcccacaat accggcaatt gcagcggcag ccagataaag ccgcagaaac
     2341 ccaccagttg taacgccagc gccagcatta acagtttgcg ccgatcctga tggcgagcca
     2401 tagcaggcat cagcaaagct cctgcggctt gcccaagcgt catcaatgcc agcaaggaac
     2461 cgctgtactg cgcgctggca ccaatctcaa tatagaaggc gggtaaccag gcaatcaggc
     2521 tggcgtaacc accgttaatc agaccgaagt aaacacccag cgtccacgcg cgaggagtga
     2581 aaaccacgcg aaccggaatg gttgttgtct tgtgggaaga ggcgacctcg cgggtgcttt
     2641 gccaccacca ggcaaagagc gcaacaacgg caggcagcgc ccaccaggcg agtgtttgat
     2701 accaggtttc gctatgttga actaaccagg gtgtcagggc ggcaccaagc ccaccgccgc
     2761 ccatcagagc cgcggaccac agccccatca ccagtggcgt gcgctgctga aaccgccgtt
     2821 taatcaccga aggcatcacc gcctgaatga tgccgatccc caccccaccg agcagtgcgc
     2881 tgctaagcag cagcgcactt tgagggtaaa gctcacgcat caatgcaccg acggcaatca
     2941 gtaacaaact gatggcgacg ctgcgacgtt cgctgacatg ctgatgaagc cagcttccgg
     3001 ccagcgccag cccgcccatg gtaaccaccg gcagagcggt caacagggca gccacgctaa
     3061 agctcattcc gcttgcctgg cgcaattgcg gtagcagtgg cccgacggag gtgagcagtg
     3121 gtcgcatatt aagaccaatc agcaccagaa ccagcagcat cgtgaacgtc ttttgatggt
     3181 tacgcatggc tgtcagaacg gtttggtcgg cagatattta ccatctaagg tgatgaccgc
     3241 acgttcgcca ccttccgggt ccgccacttt cttaacgtcg agtttgaagt taatcgcgct
     3301 gataatgcca tcgccaaatt tctcatgaac caacgctttc agggttgtgc cgtacacctg
     3361 caacatttca tagaaacgat acatcgttgg gtcggttgga atacggtcat caatgcagcc
     3421 acgcagtggg atcatctgta acagtagaat ggcgtcttcg tcgagatcca gcttcgcccc
     3481 gacctggcgg gcggcgtcgg caggaagcgc ctgctgaccc agcaaagccg cggtcacaaa
     3541 ggcttctgcc agcccggtgc cgtcggcaat ctcggcaaat gagagagctt ttttagcttt
     3601 gctgagcaaa atggcatcgg caagatcaag acgaatattg cggttaattt gcgactgaat
     3661 catggtgcat ctcctgatgg tttaaaaata aggttacgct gcggtcggtt ggcgtagcgg
     3721 tatggcacaa acgcgaggat tagctgccag tggcacaaac tggcgggttg cgccgtcaaa
     3781 cgctgcgatg ctgccgcttt caatgtcgta gacccagccg tgcagggcga tccgcccctc
     3841 ttcgagcgcc aggcgcaccg atggatgagt ttgcaaatta gccaactgag caatgacgtt
     3901 ttcacgtacc atcgccgcag cttttgacgg taaatcggaa tgcgggcgcg cctcattaac
     3961 gacgcgggct gaatcggcat aacgcagcca gtgggagacg gcaggcatat ggtccatgca
     4021 ctgacagctg gcaatggcgg tcatcgcgcc acagttggaa tgaccgcaaa tcacaatgtc
     4081 agatacccga agcgcagcga cggcatactc caccgaagca gaaacgccac cgggttccgg
     4141 cccgtaggaa gggacgatat tgcccgcatt gcgaataacg aaaagatcgc caggctcacg
     4201 ttgcgtcacc agctcaggga ccagacggct gtcggagcag gagataaaaa gtgtgcgcgg
     4261 gctttgctgt gtcgccaact gtttaaataa ggcttcccgc ttcggaaatg cctcgcgctg
     4321 gaatttaagg aatccatcaa taatctcttt caccgttaac ctctatctgt ctctggaatg
     4381 agaggcagac tactcgccga ttgtcataag gtaaaagtct catttatgat gagttctatt
     4441 gaatttactt ataggttggc gaatgctctc tcgacatatc aattattttc ttgccgtggc
     4501 tgaacatggc agcttcaccc gtgccgccag tgcgttgcac gtctctcagc ctgcgctttc
     4561 ccagcagatt cgccagttag aggagagttt aggcgtgccg ctgtttgacc gtagcgggcg
     4621 aacgattcat ctcactgatg caggagaagt ctggcgacag tacgccagcc gggcgttaca
     4681 ggaactggga gcgggtaaac gggcgattca tgatgttgcc gatctgacga gaggatcgct
     4741 gcgtatcgcc gtcaccccca cctttacgag ctactttatc ggccccttaa tggcggattt
     4801 ctatgcgcgc taccccggca tcacgctcca gctacaggaa atgtcgcagg agaaaatcga
     4861 agatctgctt tgtcgcgacg agctggacgt tgggattgcc ttcgcgcctg tgcatttgcc
     4921 ggagctggag gcaattcctt tactgacaga aagtttagcg ttagtcgtgg cgcaacatca
     4981 tccgctggct gcctgtgaac aggtggcgtt gagtcgcttg catgatgaaa aactggtcct
     5041 gctcagcgcg gaatttgcca ccagagagca gattgaccac tactgcgaga aagcggggct
     5101 gcatccacag gtggtcattg aggcgaactc aattagcgcg gttctggagc tgattcgccg
     5161 cacttccctt tccacattgt taccagcagc gattgccaca caacatgacg ggcttaaagc
     5221 gattcctctt gccccgccac tactggagag aacggcggtt ttgttgcggc ggaaaaatag
     5281 ctggcagaca gccgccgcga aggcattttt gcacatggcg ttggaggaat gtgcggatgt
     5341 tggcgagaat gaatcgcggt aaccactgac acaattgcct gatgcgttac gcttatcagg
     5401 cctacgagtt cagtgctcta cgtaggcctg ataaggcgtt caagccgcat ccggcataaa
     5461 ctacgctcgc tttgtcaaca cacagcccag ccgatcaccg tttgtaatcg atggcttccg
     5521 gctgctccag atatacggtg gtttgtgccg gttgtgtgct ggcaatcacc ttgccgccac
     5581 gtaccgaata acgtaccgga acctgacggc gcagcgcatc aaatccattt tcagccggca
     5641 ggataatcag gttggcgctg tttccggcgg caatgctgta atcctgcaaa ttcaacgtcc
     5701 tggcgctgtg gtgggtgatt aaattcaggc catcgttaat ctgcccatag cccatcagct
     5761 ggcaaacatg cagccccata tgcagcactt gcagcatatt cgccgttccc agcggatacc
     5821 acggatcgaa gacatcatcg tgaccaaagc agacgttaat gccggactcc agcatctctt
     5881 taacgcgcgt gatgccgcga cgttttggat acgtatcgaa tcgtccttgc agatgaatat
     5941 tgaccagcgg gttggcgaca aagttaatac cggacatttt cagcaagcgg aacagacgtg
     6001 aggtatacgc cccgttataa gagtgcattg ccgtggtgtg gctggcggtg actcgcgcgc
     6061 ccatgccttc acggtgcgcc agggcagcaa cggtttcgac aaagcgcgac tgctcgtcat
     6121 cgatctcatc acagtgaacg tcgatgagac ggtcgtattt ttgcgccagg gcgaaggttt
     6181 tatgcagcga ctccacgccg tattcacggg taaattcaaa atgcggaatc gcccccacta
     6241 catctgcccc taagcgtaac gcctcttcca gcaacgcttc accgttggga tacgacaaaa
     6301 tcccttcctg agggaaggcg acgatttgca gatcaatcca cggcgcgact tccagcttca
     6361 cttccagcat tgctttcagc gcagttagcg ttgcatccga aacatcgaca tgggtacgca
     6421 catgctgaat gccgttggca atctgccatt tcagcgtttg ccatgcgcgt tgtttcacat
     6481 cgtcatgggt taataacgct ttgcgctcgg cccagcgttc aatgccttca aacagcgtgc
     6541 cggactgatt ccagttcggt tgtccggcgg tttgcgtggt gtccaggtga atatgtggct
     6601 ccacaaacgg cggtataact aaaccttgtt cggcatccag gctgttttca gttatgggca
     6661 tcacgccgga ttgcgcatca atggcgctga tttttccgtc ctgcagatga atctgccaca
     6721 gcccctcttt gcctggcaac cgggcgttaa taattgtttg taaagcgtta ttcgacactg
     6781 ttagcctcca catgcgtcat tgctgctgtc gttttacgat tcaaaatcgg gttaaggatc
     6841 agatagctca gcgcgccacc taataccgcg ttgaccggaa caattcccgg taaccagtgg
     6901 cctgcagcaa tccccagggc gaccgccaga atcgccaccc aattgacact catcatacgc
     6961 gtggtcgcaa agtgctcata gcggcgacgg ttcatcagat agtcggcaat gatcacgcca
     7021 cccactggag gaatagctgc cgaaaggaag gtcaaccagc cgacaaaatt gttatacagc
     7081 cataatgcac agaccgtacc gataataccg ttgattaccg aaagggtttt gctcgacatc
     7141 ccggtaatgt tggcgaaacc taaacccgac gcatagagcg cgttatcgtt ggtggtccag
     7201 atatttagcc ccagcaccac aatcgcaggc aacagcaggc cctgagcaat catcacatca
     7261 gagatatccg ccatccccag tgccgcagcg cccgctgcac cgaaaataaa catcaacgag
     7321 ttgccgagga aaaaggccac catcgccacc agcaccgcca gtttggcatt gcgaccaaag
     7381 cggacaaagt cagcggtgag cgtgcccgca ctgataaatg accccacaac cagcgccagc
     7441 gcgacattga aatctaacgg ttgtgcggga acgaccgctt ttaaggcgtc caggccgccc
     7501 atgccgttaa cggccagcca cacggaataa ccgccaagac aggcgatagc cggaaccgca
     7561 atcaacgaaa gaaccgtcag cgccgaaatg ccaaagaaga cggtgacggt catcagtaaa
     7621 ccggaaacgg caatcagcaa attaatatcc agcccggttg ccttgcccac cggaatagca
     7681 aacatcgcca caccgacgcc aaaccagcca acctgggtgc cgcccagcag cagtgaaggc
     7741 agccatgagc ctttaacacc aaacgagaag cgagcaagaa gatgagtggt caggccggtt
     7801 tttgcgccaa tgtaaccgag aaatgaagtg taaataccga ggagaagatt accgatgaga
     7861 actgcgagga agaaatcatg atagctaaga ccggttccga gagtgccgcc ggtccacata
     7921 ctggcggaaa agaaggttaa tcccagcatg acgaacgtca atgccaatac ccctttccgc
     7981 gccgactgcg ggaccggccc ctggctaaag ttgttatctt gcgacacgaa attccccctt
     8041 tttgctgttt gaaacccaaa aaatccgccg cattctattc atctgaagat aaaaagcaat
     8101 cgttttcgtg gggaaatata ttttttatat gaatgagtgc aaaaaggtgg ggggaaaatt
     8161 atcggtgtaa aaacagtggg taggcatgat aagacgcgtc agcgtcgcat caggcaacta
     8221 cgcacggtgt cggatgcggc gcgagcgcct tatccgacct acggttcagg cctgtaggca
     8281 tgataagacg cgcctgcgtc gtatcaggca tctgcgcacg gtgtcggatg cggcgcgagc
     8341 gccttatccg acctacggtt caggcctgta ggcatgataa gacgcatcag cgtcgcatca
     8401 ggcactgagc acggtgtcgg atgcggtgcg agcgccttat ccgacctacg gttcaggcct
     8461 gtaggcatga taagacgcat cagcgtcgca tcaggcatct gcgcatggtg tcggatgcgg
     8521 cgcgagcgcc ttatccgacc tacggttcag gcctgtaggc atgataagac gcgcctgcgt
     8581 cgcatcaggc atcggcgcat ggtgtcgaat gcggcgcgag cgccttatcc gacctacggt
     8641 tcaggcctgt aggcatgat
//
LOCUS       DAZKFZ010000191         8599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000191 DAZKFZ010000000
VERSION     DAZKFZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(177..389)
                     /gene="rpmE"
                     /locus_tag="ACHOQW_003845"
     CDS             complement(177..389)
                     /gene="rpmE"
                     /locus_tag="ACHOQW_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP9574842.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            592..2790
                     /gene="priA"
                     /locus_tag="ACHOQW_003846"
     CDS             592..2790
                     /gene="priA"
                     /locus_tag="ACHOQW_003846"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9574843.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2946..3971
                     /gene="cytR"
                     /locus_tag="ACHOQW_003847"
     CDS             2946..3971
                     /gene="cytR"
                     /locus_tag="ACHOQW_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP9574844.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4063..5022
                     /gene="ftsN"
                     /locus_tag="ACHOQW_003848"
     CDS             4063..5022
                     /gene="ftsN"
                     /locus_tag="ACHOQW_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP9574845.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5115..5645
                     /gene="hslV"
                     /locus_tag="ACHOQW_003849"
     CDS             5115..5645
                     /gene="hslV"
                     /locus_tag="ACHOQW_003849"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP9574846.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5655..6986
                     /gene="hslU"
                     /locus_tag="ACHOQW_003850"
     CDS             5655..6986
                     /gene="hslU"
                     /locus_tag="ACHOQW_003850"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP9574847.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7053..7979
                     /gene="menA"
                     /locus_tag="ACHOQW_003851"
     CDS             7053..7979
                     /gene="menA"
                     /locus_tag="ACHOQW_003851"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9574848.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8072..8557
                     /gene="rraA"
                     /locus_tag="ACHOQW_003852"
     CDS             8072..8557
                     /gene="rraA"
                     /locus_tag="ACHOQW_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP9574849.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2089 a         2229 c         2349 g         1932 t
ORIGIN      
        1 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
       61 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      121 ggcaaattac aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      181 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      241 tcacgctgtt tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      301 tgaccaacgg tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      361 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      421 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      481 cgtacgaagg cgcgaaatca tacagaaatt aaccagcata tgcaaactga tccgcactct
      541 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      601 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctcct gccagaaggc
      661 atggcggtta aagccgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      721 ggggttgtgg tgtcagttag cgacgtcagt gagctgccgc ttaacgagct aaaagcggta
      781 gtcgaagtgc tggatagtga gccggtgttt actcactccg tctggcgatt gctgctctgg
      841 gcggcggatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      901 ctgcgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
      961 ggccatgcgg tggatctgaa cagcctgaaa cgttcgccaa agcaacaaca ggcactggcg
     1021 gcattgcggc aaggcaaaat ctggcgcgac caggttgcca cgcttgaatt taatgatgcc
     1081 gcgttacagg cgctgcgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1141 agcgactggc gaacgaacta tgccgtttct ggtgaacggt tgcgattgaa taccgaacag
     1201 gccaccgccg ttggcgcaat tcatagcgcg gcggatacct tctctgcttg gctgctggcg
     1261 ggcgttacag gttccggtaa aacagaggtt tatttgagcg tactggaaaa cgtgctcgct
     1321 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1381 cgttttcgtg aacgttttaa tgcccccgta gaagttctgc attccggcct gaacgacagc
     1441 gagcgtcttt cggcgtggtt gaaagcgaaa aatggtgagg cggcgattgt catcggcacc
     1501 cgctccgcgc tgtttacgcc gtttaaaaat ctcggtgtga ttgtcattga tgaagagcac
     1561 gacagttcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtttat
     1621 cgtgcgcaca gcgagcaaat cccgattatt cttggctccg caacacccgc actggaaacg
     1681 ttatgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacgcgtcg ggcggggaat
     1741 gcgcgtccgg caattcaaca tgtgctggat ttaaaaggtc agaaggtgca ggcggggctg
     1801 gctccggcgt taatcacccg tatgcgccag catttacagg ccgacaacca ggttatcctc
     1861 tttcttaacc gccgtggctt tgcgcctgcc ttgctgtgcc acgactgcgg ctggattgcc
     1921 gaatgtccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     1981 caccactgtg acagccaacg cccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
     2041 ctggtacccg tggggctggg caccgaacag cttgaacaga cgctcgcgcc gttattccct
     2101 ggtgtgccca tttctcgtat cgaccgcgat accaccagcc gcaaaggagc gctggaacaa
     2161 caactggcgg aagtgcatcg cggcggcgcg aggatattga ttggcacgca aatgctggcg
     2221 aaaggtcacc actttccgga tgtgacgctg gtagcgttac tggatgtgga cggcgcgctg
     2281 ttttctgccg attttcgctc ggcagagcgt tttgcccagc tatacaccca ggtagcaggc
     2341 cgtgccgggc gtgcgggtaa acagggcgaa gtggtgctgc aaacgcatca tccggaacac
     2401 ccgctattgc aaacgttgct ctataaaggc tacgacgctt ttgccgaaca ggcactggct
     2461 gagcggcgaa tgatgcaact gccgccgtgg accagccatg tgattgtgcg tgcggaagat
     2521 cataacaatc agcacgcgcc attgttcctg caacaactgc gtaatctgat cctctccagc
     2581 ccgctggcag acgacaaatt gtgggttctc ggtccggttc cggcactggc acctaaacgt
     2641 ggcggtcgct ggcgctggca gatattgttg cagcaccctt cccgcgtgcg cttgcaacac
     2701 atcattaacg gtacgctggc actcatcaat acaataccgg attcccgtaa ggtgaaatgg
     2761 gtgctggatg ttgatccgat tgagggttaa accgctcacg atgcgaggcg gatcgaaaaa
     2821 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2881 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag
     2941 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3001 caaaagtctc tacagcgacc gtctcccgag cattaatgaa tcccgataaa gtctcccaag
     3061 ccacccgtaa tcgggttgaa aaagcggccc gtgaagtggg ttatttaccg cagcctatgg
     3121 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3181 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3241 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3301 tcatcaccaa gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca
     3361 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac
     3421 tggagctgcc taccgttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatt
     3481 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccgctgt
     3541 gtcactaccg cctgcaaggt tacgttcagg cgctgcgtcg ctgcggcatt atggttgatc
     3601 cgcaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagcg atgcagcagc
     3661 tgcttgatct tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg
     3721 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3781 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3841 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3901 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     3961 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4021 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4081 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4141 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4201 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4261 agccagaaag tgaccggaaa cggactacca ccaaaaccag aagaacgctg gcgctacatc
     4321 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt
     4381 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4441 gctgatatgc gccagcagcc aacgcagctg gttgaagttc cgtggaatga acagacgccg
     4501 gaacagcgtc agcaaacgct acagcgccaa cgtcaggcgc agcaattagc ggaacagcaa
     4561 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg
     4621 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagccg
     4681 taccaggatc tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct
     4741 gcgccggtta ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4801 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4861 cagctggcgt tcgaaggctt tgactcaaaa attaccacca acaatggctg gaatcgtgtg
     4921 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     4981 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5041 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5101 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5161 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5221 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5281 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gtcaaagccg
     5341 ccgttgagct ggcaaaagac tggcgtaccg atcgcatgtt gcgcaaactt gaagcactgc
     5401 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5461 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgtg
     5521 cactgttaga aaatactgaa cttagtgctc gtgaaattgc tgaaaaggcg ttggatattg
     5581 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5641 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5701 acatcatcgg ccaggacaac gccaagcgtt ccgtggcgat tgctctgcgt aaccgctggc
     5761 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5821 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5881 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctacgtc ggtaaggaag
     5941 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6001 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6061 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6121 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6181 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6241 ccagccagct gcagtccatg ttccagaacc tgggcggtca gaagcaaaaa gcgcgtaagc
     6301 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6361 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6421 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6481 gcgttcagcg agacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6541 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6601 ctgacctgat cccggaactg caaggtcgac tgccaatccg cgttgaactg caggcactga
     6661 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6721 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaaacgca
     6781 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6841 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6901 aaaatatcac tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     6961 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7021 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7081 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
     7141 tcgggacggc gctggcatgg tggcaaggtc attttgatcc gctggtcgcc ctgctggcac
     7201 tgattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
     7261 taaaaggcag cgataaacct gaccgtatcg gaccgctacg cggcatgcaa aaaggggtca
     7321 ttacccagca agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
     7381 ggctggcact ggttgcagtg gcgtgccata cgctggccga ttttgtcggt ttcctgattc
     7441 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
     7501 atatcggtct gggtgatatt tccgtactgg ttttctttgg ctggttaagc gtcatgggga
     7561 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcatgcg
     7621 gcctgctggc aacggcagta ctgaacatta ataacctgcg tgatatcaat agcgaccgcg
     7681 aaaacggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
     7741 cctgcctgct gatgggctct ctggtatgtc tggcgctgtt taatctcttt tcgctgcata
     7801 gcctgtgggg ctggctgttc ctgctggcgg caccgttgct ggtgaagcaa gcccgttatg
     7861 tgatgcggga aatggacccg gtggcgatgc gaccgatgct ggaacgcact gtcaagggag
     7921 cgttactgac taacctgctg tttgttgtag ggatattcct aagccagtgg gcagcataac
     7981 tgacaaatat caattaacaa ttgatgattt tgccaacaga ccacatagcg cgatatactg
     8041 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
     8101 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
     8161 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
     8221 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
     8281 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
     8341 ggtcatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
     8401 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
     8461 cgtcaatttt ggcggtgtta ccttcttctc cggcgaccat ctttatgccg acaataccgg
     8521 gattattctt tcagaagatc cgctggatat tgaataagta tctgccatgc gttaaaaggg
     8581 gaattaaatt ccccttttt
//
LOCUS       DAZKFZ010000192         8556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000192 DAZKFZ010000000
VERSION     DAZKFZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            122..556
                     /gene="slyA"
                     /locus_tag="ACHOQW_003853"
     CDS             122..556
                     /gene="slyA"
                     /locus_tag="ACHOQW_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP9574850.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(603..1070)
                     /gene="slyB"
                     /locus_tag="ACHOQW_003854"
     CDS             complement(603..1070)
                     /gene="slyB"
                     /locus_tag="ACHOQW_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP9574851.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1343..2452
                     /gene="anmK"
                     /locus_tag="ACHOQW_003855"
     CDS             1343..2452
                     /gene="anmK"
                     /locus_tag="ACHOQW_003855"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP9574852.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            2550..2879
                     /gene="mliC"
                     /locus_tag="ACHOQW_003856"
     CDS             2550..2879
                     /gene="mliC"
                     /locus_tag="ACHOQW_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP9574853.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2938..3594
                     /gene="pdxH"
                     /locus_tag="ACHOQW_003857"
     CDS             2938..3594
                     /gene="pdxH"
                     /locus_tag="ACHOQW_003857"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP9574854.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3723..4997
                     /gene="tyrS"
                     /locus_tag="ACHOQW_003858"
     CDS             3723..4997
                     /gene="tyrS"
                     /locus_tag="ACHOQW_003858"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9574855.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5059..5919
                     /gene="pdxY"
                     /locus_tag="ACHOQW_003859"
     CDS             5059..5919
                     /gene="pdxY"
                     /locus_tag="ACHOQW_003859"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFP9574856.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(5963..6568)
                     /gene="gstA"
                     /locus_tag="ACHOQW_003860"
     CDS             complement(5963..6568)
                     /gene="gstA"
                     /locus_tag="ACHOQW_003860"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP9574857.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6674..8176)
                     /gene="dtpA"
                     /locus_tag="ACHOQW_003861"
     CDS             complement(6674..8176)
                     /gene="dtpA"
                     /locus_tag="ACHOQW_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP9574858.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         2224 a         2097 c         2160 g         2075 t
ORIGIN      
        1 tctgaaaaat atgtctgacg gtaaccaaat gcagcaatac atttgtttta gcaatacaat
       61 tgctgcacac tattctaaaa gccgcataat atcttagcaa gctaattata aggagatgaa
      121 attggaatcg ccactaggtt ctgatctggc acggttggtg cgcatatggc gtgctctgat
      181 agaccatcgc ctgaaaccgc tggagttaac acaaacccat tgggttacgt tacacaatat
      241 ccatcagtta cctccagacc agtcgcaaat tcaactggca aaagcgattg gcatcgagca
      301 gccatcactg gtccgtactc tggaccaact ggaagaaaaa gggttaattt cgcgtcaaac
      361 ttgtgccagc gatcgtcggg ctaaacgtat taaactgacg gaaaaggcag agccgctgat
      421 cagcgaaatg gaagctgtta ttaacaaaac ccgcgcggaa attttacatg gcatctccgc
      481 agaggaactg gaacaactga ttacgctcat cgcaaaactt gagcataata tcattgagtt
      541 acaggccaaa gggtgaaatg aagggggcgt gtggtcagta acctgaccac acgcaaactt
      601 atttagcgcg gagaaacggt cacctgactg ccattgctgg ccagtacgac acgttggccc
      661 ggagagaaac gagtgttgcc ttgtttctgt accaccatga tggtattacc atcgtcttta
      721 cgaatttcca gctcgacacc ttgcgttttg ttcattgcac tctgtacgcc ctgaccagct
      781 acgccacctg caacagcgcc tgctgcagta gccagagaac gcccggttcc gccaccaaca
      841 gtatttccca ggaaaccacc aagaacagca ccgccaattg caccgataac gttggaatca
      901 tcaccgccct gaatctgtac cggacgtacg ttaacgatgg ttccatagct gacattctgt
      961 acttgtttcg cttcagaagc ggtataaaca tcccctgaca gggtgtcgtt attaacacaa
     1021 ccgacaagag acagacctac cattgaaaca accaatacgc gtttaatcat tgaaaaatct
     1081 cctgttcacc atgaaacgcc acgcaagcat ccctcatggt caaagtatat ggcatatgtg
     1141 aatccatggt tacatgttct accaaatatg agcaaatcat aatgaaagta ggatcaacca
     1201 attataaaca aaacattcat gatgattatt gatttgaaga taatgtttta ctaccaacgg
     1261 taaaaatcgg tcattccgca tggtattcac gaaaagttta tgtttgagtg tgcgcaaacg
     1321 tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac
     1381 cagccttgat ggtgttgatg ttgtgttggc gacaattgat gaacaccggg tcgcacagct
     1441 ggcaagtttg agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca
     1501 gggccagcag cttacacttt cgcagtttgg acagcttgat actcaactcg ggcgactttt
     1561 tgctgatgcg gtcaaggcct tgcttaagga acaaaacctg caggcgagag atatagttgc
     1621 gatcggctgt cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac cacacacttt
     1681 acagattggc gataacaatc aaattgtggc acgcaccgga attacggttg tcggtgattt
     1741 tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg ctggtacctg cgttccatca
     1801 tgccttgctg gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgccaa
     1861 tctgtcactg ctcattcctg ggcagccggt tggcggctac gataccggtc ctggtaacat
     1921 gctgatggat gcctggatct ggcgtcaggc cggtaaacct tacgataaag atgccgagtg
     1981 ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccatattt
     2041 ctcgcaacct gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg
     2101 ccatttacgc cattttccgg gtgttgatcc ccgtgatgtt caggccacac tggcagaact
     2161 caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt
     2221 atgtggtgga ggtagtcgta atccgctact catggcgcgt ctggcggcat tactgccagg
     2281 cacagaagtc accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc
     2341 tttcgcttgg cttgcctggc ggacgctggc gggattacca ggaaatctgc cttccgtcac
     2401 tggcgcaagc caggagacgg ttctgggggc tattttcccc gccaactcgt gatatcagaa
     2461 ttaactgaat tttttcttat cgtgtctgcc gttacacttg gaagattaag gaggatattt
     2521 gccctccgga ccaggaaagt cttcgggata tgactatgaa aaaactgtta attatcattt
     2581 tgccggtcct tctctccggc tgtagcgcct ttaatcaact ggttgagcga atgcaaaccg
     2641 atacgctgga ataccagtgt gatgaaaaac cgttgacggt caaactgaat aatccgcgcc
     2701 aggaagtcag ttttgtttac gataatcaac tactgcatct caaacagggc ctttcagcct
     2761 ctggcgcgcg ttactccgac gggatctatg ttttctggtc gaaaggtgag gaagcgactg
     2821 tctataaacg cgaccgcatc gtcttgaata actgtcagtt acaaaatcca cagcgttgag
     2881 atttttccag gggcggcgca caatagcgtc acccacagac aatccgtaaa gaaaaccatg
     2941 tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcggg
     3001 ttacgccgcc gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg gctctctcag
     3061 gcttgtgaag ccaaactggc ggaccctacc gcgatggtgg tcgctaccgt ggatgaacat
     3121 ggtcagcctt atcagcgcat cgttttactc aaacattacg acgaaaaagg tatggtgttt
     3181 tacaccaacc tcggtagccg taaagcacat caaattgaaa ataatccgcg cgttagcctg
     3241 ctgttcccgt ggcataccct tgaacgccag gtgatggtga tcggtaaagc agaacgactt
     3301 tcgactctcg aagtgatgaa atattttcat agccgcccgc gtgatagcca gattggttca
     3361 tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg
     3421 gagctgaagc agaagtttca acaaggcgaa gtgccattgc cgagcttttg gggcggtttt
     3481 cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc
     3541 tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat
     3601 gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt
     3661 tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt
     3721 tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga
     3781 cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct
     3841 tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac
     3901 gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg ggtctgattg
     3961 gcgacccgag cttcaaagct gccgagcgta agctgaacac cgaagaaact gttcaggagt
     4021 gggtggacaa aatccgcaag caggttgccc cgttcctcga tttcgactgt ggagaaaact
     4081 ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc
     4141 gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc
     4201 gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg
     4261 gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt ggtggttctg
     4321 atcagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg
     4381 tgtttggcct gaccgttccg ctgatcacca aagcagatgg caccaaattt ggtaaaactg
     4441 aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct
     4501 ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc tttatgagca
     4561 ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc
     4621 agtatgtact ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt ttacaggcgg
     4681 caaaacgtat taccgaatgc ctgttcagcg gttctttgag tgcgctgagt gaagcggact
     4741 tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc gcagacctga
     4801 tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg
     4861 cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag
     4921 aagaagatcg tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc
     4981 tgatttgctg gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt
     5041 ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt
     5101 catgcgggta acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg
     5161 ctgaacaccg ttcaattttc taatcacacc caatacggca aatggactgg ctgcgtgatg
     5221 ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc
     5281 tgtgatgccg tattaagtgg ctatctggga tcggcggagc agggtgaaca tatcctcggt
     5341 atcgtccgtc aggtgaaagc agcgaatccg caggcgaaat atttttgcga tccggtaatg
     5401 ggtcatccag aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac
     5461 ggtttgcctg ccagcgatat cattgcgccg aatcttgttg agctggaaat actctgtgag
     5521 catccggtaa ataacgtcga agaagcggtt ctggcagcgc gcgaactcat tgcgcaaggg
     5581 ccacaaattg tattggttaa acacctggcg cgagctggct acagccgtga ccgttttgaa
     5641 atgttgctgg tcaccgccga tgaagcctgg catatcagcc gtccactggt ggattttggt
     5701 atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt
     5761 cagggggcaa cgctgcagga ggccctggaa catgtgaccg ctgcagtcta cgaaatcatg
     5821 gtgaccacca aagcaatgca ggaatatgag ttgcaagtgg ttgctgctca ggatcgtatt
     5881 gccaaaccag aacattactt cagcgcaaca aagctctgaa atatctcatt tcggcccaca
     5941 acgaactgtg ggccgacggc tattacttta agccttccgc tgacagcgcg tcttgtactt
     6001 ccggacgttc agccatacgt tgcataaatg ccgcaatgtg ctctaaccct tccagattca
     6061 gtttcaccgc gtatgcccag cgcagtaccg taaacagata ggcatcagca attgtaaatc
     6121 tttgcccgca gatccagtgc tcatccttca gtgcctcgtt cacatattgc agtttcttct
     6181 ccagctgcgc gcgaactgtc ggtttgtact cttccggtgt atcagggcga aacagaggtg
     6241 tgaaaccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg
     6301 aaatactgtt taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca
     6361 taatcgctac accttccgtc agcaaagtac cgtcatccag cagcaatgca ggtacctgtc
     6421 ccttagggtt aacggcaaag taatcgtcac cgttttcgag acgttttttc attaaatcca
     6481 cactgacgag cgtaaaatcc tttccgctct cacgcagggt gatatgggaa gcgagagagc
     6541 aggcgcccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat
     6601 ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga
     6661 gtttccctga attttacgct acggctgctt tcgccgcttt gtctgcagcg tcatcctgcg
     6721 tcatgcggtg cagtttcggc gcggtcagca gcatcagtac tgcaataacg gcagtagcga
     6781 caccaatctg caagaatacg cgaccataga cttccagtga catcagcgga tcggtaacgt
     6841 tatccggcac agccatcata cccgcaacat aaccaccaat caggtttgca ccggcagtgg
     6901 tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca
     6961 ttgccagacc cagaccagag atcatcagtt ccccgatact ctgcaggcca tagctcgcga
     7021 ccagccagct tacagacacg atacctgcgt cagacgcgaa tttcgcaccc agcggcagaa
     7081 tcaggaacgc accagaacac atcaccatac cgattgcaaa cttggttggc atcggcaggg
     7141 tatcgcccat cttgttatag atagcggcca gaatcgggct accaatgatg atccagaacg
     7201 ggttcagtgc ctgatactgc tcaggttcta cggccaggcc cagaatggta tgttcaacgt
     7261 tacgaatcgc aaagaagttc agtgacgttg gcatctggct gtacagcacg aagaagataa
     7321 tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg
     7381 cgaaggcttc tttaccgaag ataaccacga taccgaaggc aacaacgccc agcgccatac
     7441 gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa
     7501 taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact
     7561 gtttaaccca gcgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc
     7621 tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggggtg gcgatcatag
     7681 agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgcg
     7741 ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcttta aacaggccgt
     7801 taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga ccagaccagg
     7861 caaccagagc ataaccaatc gccagcacaa tagcgccgag cataattacg cgtttagtac
     7921 ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa
     7981 aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa
     8041 cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga
     8101 tgagatagaa cgccttcggt tgtttgaaag cgttcaaact gacgctttca gttggttttt
     8161 ggtttgcagt ggacactttt acctcttttt ttacatccca tattaacggg ggtgctttct
     8221 cgcgttatgt ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa
     8281 tgttcactat cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag
     8341 gcggggaatt caaccattcc gggaaatgtt atccagcgtt aaatttttca aaaaacgtaa
     8401 cggcagattt tttcactgca ttgagagggg ggtgatggct tgacggcgat atgttctgct
     8461 atttagcgtt tttcctagtg agatagtcca gtttcttaaa aatagccagt gtaatgtttt
     8521 gtaggtcaat atttgttgcg tgtgtaaatt taaatg
//
LOCUS       DAZKFZ010000193         8550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000193 DAZKFZ010000000
VERSION     DAZKFZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..72
                     /locus_tag="ACHOQW_003862"
     CDS             <1..72
                     /locus_tag="ACHOQW_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574859.1"
                     /translation="WVPEGKLYFALMNVLQMFPMKNG"
     gene            197..574
                     /locus_tag="ACHOQW_003863"
     CDS             197..574
                     /locus_tag="ACHOQW_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574860.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            690..1490
                     /gene="ydcF"
                     /locus_tag="ACHOQW_003864"
     CDS             690..1490
                     /gene="ydcF"
                     /locus_tag="ACHOQW_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP9574861.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            1687..3126
                     /gene="aldA"
                     /locus_tag="ACHOQW_003865"
     CDS             1687..3126
                     /gene="aldA"
                     /locus_tag="ACHOQW_003865"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP9574862.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3168..4169)
                     /gene="gap"
                     /locus_tag="ACHOQW_003866"
     CDS             complement(3168..4169)
                     /gene="gap"
                     /locus_tag="ACHOQW_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9574863.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            4358..4888
                     /gene="cybB"
                     /locus_tag="ACHOQW_003867"
     CDS             4358..4888
                     /gene="cybB"
                     /locus_tag="ACHOQW_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP9574864.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            5133..5306
                     /locus_tag="ACHOQW_003868"
     CDS             5133..5306
                     /locus_tag="ACHOQW_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574865.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            5332..5400
                     /gene="yncP"
                     /locus_tag="ACHOQW_003869"
     CDS             5332..5400
                     /gene="yncP"
                     /locus_tag="ACHOQW_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP9574866.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(5378..5527)
                     /gene="hokB"
                     /locus_tag="ACHOQW_003870"
     CDS             complement(5378..5527)
                     /gene="hokB"
                     /locus_tag="ACHOQW_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP9574867.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            5926..7566
                     /gene="trg"
                     /locus_tag="ACHOQW_003871"
     CDS             5926..7566
                     /gene="trg"
                     /locus_tag="ACHOQW_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP9574868.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            complement(7604..8527)
                     /gene="ydcI"
                     /locus_tag="ACHOQW_003872"
     CDS             complement(7604..8527)
                     /gene="ydcI"
                     /locus_tag="ACHOQW_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9574869.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         2239 a         1952 c         2153 g         2206 t
ORIGIN      
        1 tgggtgcctg aggggaaatt gtatttcgca ttaatgaatg tattgcagat gtttcccatg
       61 aaaaatggtt aataactgga caagataatt ttaaaattca gcaacggtta tcgattatac
      121 atggtactta aacagactca caccattttt gtagaaggtt aaattgagta cagtctcata
      181 tattaagggt gttaacatgg atgaacgttt tataaatatc acgccgactt cgttgtcttt
      241 taaacacgcc gatctgtcag gtttaagcta taagaatgcc aggcttgaac tggccctgat
      301 atgcccggcg atcaagtcag aagttaagat atcatttgac tggatacact catttcgtgt
      361 tactgacgaa ggtgatttgt taggtatgtc aggaaagcag ggaattaacc aaacggggat
      421 ctatagagta gccaattcca cttatcttac atggtttact atgcaaagtt gtgaaattca
      481 taaaaataaa atgatcgaac attatgttat tgccacgtca aatgatgtta ttgatgtttt
      541 atcaacagtt tctccgcaaa tagtgtatga ctaaaaccag tgtctggttc gcgatggcga
      601 gatagaaaaa gcattatctt cctttcagag tgctccgctt ctcactatta tctcacgcag
      661 tattcttaag ggaacgataa ggaggaacca tgaacattac cccgtttccg acgctttcga
      721 cggcaactat agatgccata aatgttatcg gacagtggct ggcgcaggat gatttctccg
      781 gtgaggtgcc gtatcaggct gattgcgtca tacttgcagg aaatgcggtt atgccgacta
      841 tcgatgcggc atgtaagatt gctcgcgatc agcaaattcc tttactgatt agtggtggta
      901 tcggtcactc gaccactttt ttgtatagcg ccatcgcaca gcatccgcac tacaacacta
      961 tccgcaccac tggcagagca gaagcgacca tcctggcgga tatcgctcat cagttctggc
     1021 atattccgca tgaaaaaatc tggattgaag accagtcaac aaactgcggt gaaaacgcac
     1081 gctttagcat cgcgctattg aatcaggccg tagaacgagt tcacacagct atcgttgtac
     1141 aggaccccac catgcagcgg cgcacgatgg cgacgttccg ccgtatgacc ggggacaatc
     1201 ccgatgtacc acgctggtta agttatcccg gattcgttcc tcagttagga aataacgcag
     1261 acagtgtaat ctttattaat cagttacaag gattatggcc ggttgagcgt tatctctcac
     1321 tactcactgg cgagctgccg cgtttacgcg atgatagcga tggctacggt ccccgcgggc
     1381 gagattttat cgttcacgtt gattttccgg cagaagtcat ccatgcatgg caaacgctga
     1441 aacacgatgc ggtgctcatc gaggcgatgg aaagtcgctc gttacgttaa aaattgcccg
     1501 tttgtgaacc acttgtttgc aaacgggcat aactcctgcc ttttatttct gcctcttatt
     1561 ccttttacac ttgtttttat gaagcccttc acagaattgt cctttcatga tgccgtctct
     1621 atgatgattg atgttaatta acaatgtatt taccaaaaac aaacatataa atcacaggag
     1681 tcgcccatgt cagtacccgt tcaacatcct atgtatattg atggacagtt tgttacctgg
     1741 cgtggagacg catggattga tgtggtaaac cctgctacag aggctgtcat ttcccgcatt
     1801 cccgatggtc aggccgagga tgcccgtaag gcaatcgatg cagcagaacg tgcacaacca
     1861 gaatgggaag cgttgcctgc aattgaacgc gccagttggt tgcgcaaaat ctccgccggg
     1921 atccgcgaac gcgccagtga aatcagtgcg ctgattgttg aagaaggggg caagatccag
     1981 cagctggctg aagtcgaagt ggcttttact gccgactata tcgattacat ggcggagtgg
     2041 gcacggcgtt acgagggcga gattattcaa agcgatcgtc caggagaaaa tattcttctg
     2101 tttaaacgtg cgcttggtgt gactaccggc attctgccgt ggaacttccc gttcttcctc
     2161 attgcccgca aaatggctcc cgctcttttg actggtaata ccattgtcat taaacccagt
     2221 gaatttacgc caaacaatgc gattgcattc gccaaaatcg tcgatgaaat aggccttccg
     2281 cgcggcgtgt ttaaccttgt tctggggcgt ggtgaaaccg ttgggcaaga actggcgggt
     2341 aacccaaagg tcgcaatggt cagtatgaca ggcagcgtct ctgcaggtga gaagatcatg
     2401 gcgactgcgg cgaaaaacat caccaaagtg tgcctggaac tggggggtaa agcaccagct
     2461 atcgtaatgg acgatgccga tcttgaactg gcagtcaaag ccatcgttga ttcacgcgtc
     2521 attaatagtg ggcaagtgtg taactgtgca gaacgtgttt atgtacagaa aggcatttat
     2581 gatcagttcg tcaatcggct gggtgaagcg atgcaggcgg ttcaatttgg taaccccgct
     2641 gaacgcaacg acattgcgat ggggccgttg attaacgccg cggcgctgga aagggtcgag
     2701 caaaaagtgg cgcgcgcagt agaagaaggg gcgagagtgg cgttgggtgg caaagcggta
     2761 gaggggaaag gatattatta tccgccgaca ttgctgctgg atgttcgcca ggaaatgtcg
     2821 attatgcatg aggaaacctt tggcccggtt ctgccggtag tcgcatttga cacgctggaa
     2881 gatgctatct caatggctaa tgacagtgat tacggcctga cctcatcaat ctatacccaa
     2941 aatctgaacg tcgcgatgaa agccattaaa gggctgaagt ttggtgaaac ttacatcaac
     3001 cgtgaaaact tcgaagctat gcaaggcttc cacgccggat ggcgtaaatc cggtattggc
     3061 ggcgcagatg gtaaacatgg cctgcatgaa tatctgcaga cccaggtggt ttatttacag
     3121 tcttaatgag tgaaagaggc ggaggttttt tcctccgcct gtgcgcgtca gagtttagcg
     3181 aatttttcga gggtgcgaat aagctgcgtg acgaagccat attcgttgtc gtaccaggcg
     3241 accgttttca ccagttgtaa atcgcccacg gcggtaattt ccgtttgcgt ggcatcaaac
     3301 accgaaccga aatggctgcc aatgatatcg gaagagacta tttcttcatc ggtataacca
     3361 aatgactcgt tattggtggt tgcttgttta agtgcgttat tcacctcttc ggcagtcact
     3421 ttttttccga gaatcgatac cagttcagtg accgaacctg ttttcaccgg cacgcgttgc
     3481 gcatgacctt tcagtttgcc gctcagttcc gggatcacca gaccaatggc ttttgccgcg
     3541 cccgtagtgt ggggaatgat attttctgcc gcggcgcgtg aagcacgtaa atctttacca
     3601 cgcgggccat ccaccagtga ctgggtgcca gtataggcat gaatggtcgt catcgtgccg
     3661 acttctatcc cgaaactgtc atgcaaggct ttggccatcg gcgcaagaca gttagtagtg
     3721 catgatgcca cggaaacaat ggtgtcgttg ccatccagag tgtcgtcatt gactttataa
     3781 acgatagttt tcatttcacc tgcaggggcg gaaatcaaca ccttcttcgc gccagcatca
     3841 agatgcgcct gcgatttctc ggcggaggta taaaagccag tacattcgac aatgatttct
     3901 gcacctttcg ctttccacgg aatattttta gcctcttttt cggcgtaaac cgcgatactt
     3961 ttcccatcaa cgataagtga atcttccgta aaatcaacgc tccaggggaa cggaccatag
     4021 tttgaatcat gtttcagcag ataggcgaga atttttgggg aagtaagatc attaatagcg
     4081 acaacgtcta tgttgctttt gacttcaagt aatcgacgca acaccagtcg accgatacga
     4141 ccaaaaccgt taataccaac tttactcatg gttttctcct gtcaggaacg ttcggatgaa
     4201 aattgatcct ttccaggctt agaccaggat ggcgggatgg gcaatctcca ttcttacagt
     4261 gaaacgtaac gtactgaaaa agggtgaaca atatttaatg aaattttgag aaaagcccgt
     4321 tatgttaacg gaaaattatg ttaaagcagg aaatgttatg gaaaataaat actcaaggtt
     4381 acaaatcagc attcactggc tggtcttttt gctggttatc gcagcgtatt gcgcaatgga
     4441 gtttcggggt ttcttcccac gtagcgatcg gccactcatc aacatgattc atgtttcctg
     4501 cggcatatca attctcgtgc tgatggtcgt tcgtcttttg ttaaggctga aatacccaac
     4561 cccgccgatt atacctaagc caaaaccgat gatgacggga ctggcgcatt tgggacattt
     4621 ggtgatttat ctcctgttta ttgcgctgcc agtgattggt ttggtgatga tgtataaccg
     4681 aggcaacccg tggtttgcgt ttggtttgac gatgccttac gcttcagagg ccaatttcga
     4741 acgggtagat agcttaaagt cgtggcatga aacgctggcg aatctgggat attttgtcat
     4801 cggtttgcac gctgcggctg cactggcaca ccactatttc tggaaggaca acacacttct
     4861 acgcatgatg ccgcgtaaac gttcctgaag gatatttaaa gaaaacgcct gtactaaaac
     4921 cgacccgtgg tacaggcgaa gaatacgggt ctacatcgga agcgcctatt atatttattt
     4981 gtatgacaaa taaaaccata atccttgccc atacgtccat ctggcttatt tttaatcaat
     5041 tcacccgatc tttgatctca tcaacggtat caaaataaac cagcgtataa cgttcaaatc
     5101 gaacataaga ttcgataaac catggaggtt atatgaaaaa actggcactt attttgttta
     5161 tgggagcgct tgtttccttt tatgccgatg ccgggcgcaa accctgttct ggttcgaaag
     5221 gggggatctc acactgtacg gcaggcggca aatttgtctg taatgatggt tctattagtg
     5281 catcgaaaaa aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatctg
     5341 ctagtcagat gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga
     5401 gcgcagcaac ctccttatca ctgtcccgga accgcagttc gtagagcgtt tgtcgggtca
     5461 ggagtgtgaa tgtcagaatc gtaatgcaga taatgagcag acacaccacc agagggttgc
     5521 gcttcatagc ctttctcctt gccggatggc gggtaagagg ctaacatctg aattgcttgg
     5581 ttcattcgtt ggcctcggtt gatagaaata tcggtcgggg ccttcgtctt tctgattccc
     5641 ggttagcctg aaaacagaaa gtctcaggca cccgtgagca tcctatgtgg tttccttagg
     5701 gacgaaaata atcacctgac gaaattgcgt gctgttttcc agaatttttc gtcattcggg
     5761 ttagccagtt taaccattcg ttactctctt cattccaatg gcattaatct tctctgcaat
     5821 aattgttgta aaaatgtgac gcaaagaggc ttttagtcgt aagtaattac cgtcaaatgc
     5881 cgatgacttt ctatcaggag taaacctgga cgagagacaa cggtaatgaa tacaactccc
     5941 tcacagcgat taggtttttt gcatcacatc aggttggttc cgttatttgc ctgcattcta
     6001 ggcggtatct tagttctatt cgcattaagt tcagccctgg ctggctattt cctctggcag
     6061 gccgatcgcg atcagcgtga tgttactgcg gagattgaga ttcggaccgg gttagcgaac
     6121 agttcagatt ttttgcgttc agcccggatc aatatgattc aggccggggc tgcgagtcgt
     6181 attgcggaaa tggaagcaat gaagcgaaat attgcgcaag ccgaatcgga gattaaacag
     6241 tcgcagcaag gttatcgtgc ttatcagaat cggccggtga aaacacctgc tgatgaagcc
     6301 ctcgacactg aattaaatca acgctttcag gcttatatca cgggtatgca acctatgttg
     6361 aaatatgcca aaaatggcat gtttgaagcg attatcaatc atgaaagtga gcagatccga
     6421 acgctggata atgcttatac cgatattttg aacaaagccg ttaagatacg tagcaccaga
     6481 gccaaccaac tggcggaact ggcccatcag cgcacccgcc tgggcgggat gttcatgatt
     6541 ggtgcgtttg tgcttgccct ggtcatgacg ctgataacat ttatggtgct acgtcggatc
     6601 gtcattcgtc cactgcaaca tgccgcacaa cggattgaaa aaatcgctag tggcgatctg
     6661 acgatgaagg atgaaccggc gggtcgtaat gaaatcggtc gcttaagtcg tcatttacag
     6721 caaatgcagc attcactggg gatgaccgta gggactgttc gacagggtgc ggaagagatt
     6781 tatcgtggca ccagcgaaat ttcagctggc aatgcggacc tgtcatctcg caccgaagaa
     6841 caagcggcgg ctatcgaaca aactgccgcc agcatggagc aactcactgc gacggtgaaa
     6901 cagaatgcgg ataacgcgca ttatgccagc aaactggcgc aagaggcttc tattaaagcc
     6961 agcgatggcg ggcagacggt ttccggtgta gtaaaaacga tgggcgctat ctccacgagt
     7021 tcgaagaaaa tttctgagat caccgccgtc atcaacagta ttgctttcca gacgaatatt
     7081 ctggcactga atgctgccgt tgaagctgcg cgagcgggtg agcaagggcg tggatttgcc
     7141 gttgtcgcca gcgaagtacg gacactcgca agccgcagcg cccaggcggc aaaagaaatt
     7201 gaaggcttga tcagtgaatc agtcaggtta attgacctgg gatcggatga ggtggcaacg
     7261 gccgggaaaa ccatgagcac tattgttgat gccgtcgcga gtgtcacaca tatcatgcag
     7321 gaaattgccg ccgcctcgga tgagcaaagt aggggcataa cgcaggttag ccaggcgatt
     7381 tctgaaatgg ataaggtgac gcaacagaat gcttctctgg tagaagaggc ctcagcggcg
     7441 gcggtgtccc ttgaagaaca ggcggcacga ttaactgagg cggtggacgt attccgtctg
     7501 aacaaacaat ctgtgttggc agaacctcgc ggagcgggtg aaccagttag tttcgctccg
     7561 gtgtgaaaat gttaaaggag ggatcgacag atcccttcac ctttcagaac ggcattgatt
     7621 ttcgaatagc gttaatcatc aactggcaac cagaagagaa cgtcgcatct acgcgggtca
     7681 gtattccaat cggttcgcct gcaccatgtc ccggaacagg cagggccacc agcgtggcat
     7741 gacgcaggtc gtcttttacc gcgccagaag ggacaaacca cacgtagtcg tattcaaccg
     7801 taagttgacg agatagcgaa gcagacagcg tttcgataca acccgaagga attttacatc
     7861 cctggctctg tactaatgca tctgaatgct ggcgtggcgc agtgccttct ggtgatacaa
     7921 cgaccggcca ttccagcacc cggcttagcg ttacgttctc ctgaagtagc ggatgattag
     7981 ggcggacaac cagcttcagc gattcaagaa acagcagttc gtaattaagc ccggtcatca
     8041 gttcaggatc tgacatccga ccaatgccga tatcgatttc cccggttttc aaacccgcca
     8101 gaatcatagg gttactcatt gtcgcaactt gcaaggtcgt ctctttttgt tgctgatgaa
     8161 actgacctat aaccgaaggt aatatcccca gtgccgcagt aggtagtgca ccaaccctga
     8221 cgacatcatt attaggacct tctttacgat gaagcgactg tccggcagtg ttgatggcgt
     8281 caagtactct gactgcatgc gttaaaaatt gttcgccggg taaggtaagt tgcgccccct
     8341 gacgaccacg ctcaaacaag cgagcgcccg tcagctgctc cagttcattt aatgtcttag
     8401 agagcgcagg ttgactcaaa ttaagggttt cagccgcgcg ccccaaagtt ccttgttgtg
     8461 cgacagctac gaatgtatga aggtggcgca aacggatgcg ctgactaaac agactatttt
     8521 tttccataag cgatgttaaa aacgaagcgg
//
LOCUS       DAZKFZ010000194         8496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000194 DAZKFZ010000000
VERSION     DAZKFZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..281)
                     /gene="cas2e"
                     /locus_tag="ACHOQW_003873"
     CDS             complement(<1..281)
                     /gene="cas2e"
                     /locus_tag="ACHOQW_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP9574870.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVAFLPV"
     gene            complement(278..1201)
                     /gene="cas1e"
                     /locus_tag="ACHOQW_003874"
     CDS             complement(278..1201)
                     /gene="cas1e"
                     /locus_tag="ACHOQW_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP9574871.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(1198..1848)
                     /gene="cas6e"
                     /locus_tag="ACHOQW_003875"
     CDS             complement(1198..1848)
                     /gene="cas6e"
                     /locus_tag="ACHOQW_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP9574872.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(1830..2576)
                     /gene="cas5e"
                     /locus_tag="ACHOQW_003876"
     CDS             complement(1830..2576)
                     /gene="cas5e"
                     /locus_tag="ACHOQW_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP9574873.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(2587..3642)
                     /gene="cas7e"
                     /locus_tag="ACHOQW_003877"
     CDS             complement(2587..3642)
                     /gene="cas7e"
                     /locus_tag="ACHOQW_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP9574874.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(3654..4190)
                     /gene="casB"
                     /locus_tag="ACHOQW_003878"
                     /gene_synonym="cse2"
     CDS             complement(3654..4190)
                     /gene="casB"
                     /locus_tag="ACHOQW_003878"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP9574875.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(4187..5749)
                     /gene="casA"
                     /locus_tag="ACHOQW_003879"
                     /gene_synonym="cse1"
     CDS             complement(4187..5749)
                     /gene="casA"
                     /locus_tag="ACHOQW_003879"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP9574876.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(5847..>8496)
                     /gene="cas3"
                     /locus_tag="ACHOQW_003880"
     CDS             complement(5847..>8496)
                     /gene="cas3"
                     /locus_tag="ACHOQW_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP9574877.1"
                     /translation="FDFWGKTRRGEKEGGDGYHLLCWHSLDVAAMGYLMVKRNCFGLA
                     DYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPELKIPEGSRKNYEKISH
                     STLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGHHGRPPDRIDELDNFL
                     PEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYISATVVLADWTGSSTR
                     FFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFPFIEHPTPLQQKV
                     LDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGLFFGLPTMATANAMYD
                     RLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAW
                     FANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCI
                     LEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHV
                     TKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQGKCIAWIRNSVDDAIKV
                     HRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVLE
                     QSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVW
                     DDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVV
                     MPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLPEKLSTRLAEESVSLW
                     LATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRQWCIEQRQDPE
                     MANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT         2026 a         2362 c         2032 g         2076 t
ORIGIN      
        1 acaggaagaa aagcaaccaa acgtaaccca tccaaatcca ccggaatacg tctgttttct
       61 ccccaggtct gaaattcaaa acccgactcg gtattggtcg cccaggccat caccacattt
      121 ccgcaaccag ccagttgggt aatttgctgc cagatcatct cccgaatacg ttttgatgta
      181 tcaccaacat acacaccggc acgcacttcc agtagccaga ttgcgagccg tccacgtaag
      241 cgcggaggga cattttctgt aacaaccacg accatgctca tccgccgcgc ccccggtgac
      301 cactatcacc cagcgtttca ggttcaggga tggcaggcgg taacatatcc ggcgcgggtt
      361 gtggtggttc aatttcacct gcagcaagga ctttctcaat taacggtatt aatttgcccg
      421 ttaacttagt gctacggaaa atatcgcgac aggctaatct gacttcttta tcaggttctg
      481 cgggttgcct cgctgctatt tcaaatgcct ttggcacaac cgaatcaaat ttaatgatat
      541 cggctatgtc ataaacaaat gaaagcggtt tgccactatg aataaatcca atagcgggcg
      601 catatcccgc ggctaatact gccgcttcag aaataccgta cagacatgat gtggcagcac
      661 tgatgcagcg attcacaaca tcgccttttt cccagtcttt aggatcgtat ttgcgaccat
      721 tccatttcac accatattgt ttcgccagta atgcataggt ctggcgaacg cgggatccct
      781 caattccccg tagctgatcc actgaacggc gagctggcgg tggctcacga aaacgtaatt
      841 catacatttt gcgcaccacc ttcaggcgta gatcttccgt taaagccagc tttgcctggt
      901 agagtaattt atctgcccgc gcccctccgg gttgtccgga agagtaaacg cgaacgcccg
      961 cttcaccgac ccagaccagc agtgttccca ccgtggcggc cagatgcacc gccgcgtggg
     1021 aaactctcgt tcccggttcg agcataatgc aggcgaccga tcccaccgga atgtgcgtgc
     1081 ggatcccggt tttgtcgatc agcacgaaag cgccgtccag tacgtcgatt tgaccgtact
     1141 ggaggaagat catagaggtg cgatctttta acgggatcgg actcagtggt acaaacgtca
     1201 cacctctgct ccgggtttga tcagcatcag accacagccg aacgcgcggc ttttgccata
     1261 cccctgactg agacgttgca gaaataaccc gggatcggtg accgtaagca tccccgtata
     1321 atccacgcta ctgaactgga tcagttgccg ggagttttcc cgccgcagtt gctgttgtcg
     1381 gtaggcatca accgaggtat ccagcagtgt aaaaccgctt ctctccccct gcgctgccag
     1441 ccagtccagc gccgcctgtt gttgatgcaa ccagacatca cttccttccg cctgccccct
     1501 cacctgccgt ttcgcctcca tcagcagatc gtggcgcttg cccgctttac agattgttgg
     1561 attcgcccgc aggttgaaac aaagttgttg tccggtacgc agctcgggca caaatgaccg
     1621 gcattcgatg gtgaacgttt cactttccgc cgggcgttcc tgcgacagta caaaaaagcg
     1681 aaacgcgccc tggagttctt cccgacgata aagaaattgc ctttctttgc cgccagggaa
     1741 gagatcccac agccactgat gcatcacata ttccccgcga tccaccaaat gcagcaactg
     1801 cgcaggcgaa agctggccgg tatgtaaggt tattcttgag aggtacatgg ttcctccttg
     1861 ctgagccacg gcccctgatt gatggtgcgt tccccaaaca gccactgctg acgatttaaa
     1921 ggaacatccc ggcgacgtaa tattttgctc gcgaccaggc cgtcgtgttc cccttcccac
     1981 cagcattcat cctgaagttt cgggagtgag actttcagtt cgcggaaact atcctgatac
     2041 tgttggtatg cgttacgtaa gacatcagac gcgttgcctt cgagcagtaa cggcgcaagt
     2101 ggtaacgcca gcggatgact ttttcgcccc agataaagcg gaaaaaccgg atgacgtaaa
     2161 ccgtcctgca actgttcaag gctgtaaggc gcatcggggg ttgttgccac cgccaccatc
     2221 caccaggcat cggtgtagta gtcgcgccgg gagataatcg cgctcagaag atcaggggcg
     2281 ctcaactctt cgcgacggct gaaataacgc gctttacgca cctcttttgg catctggacc
     2341 gtgtgataat cccgtgccca gcgcgggtta cggctggcgc aaaccaccag tgaatagtgg
     2401 cggttaaacg cgtttaatcg ttcggtatca tcacgccgaa tccctacccc ggcagccagc
     2461 agccccagca atgctgagcg cgaaggcagt tcatgggtat gacgcacttc gccgggggca
     2521 tcgacgcccc aggatgccat tggcccatga agctgaaaaa tcaaatattg gctcattagc
     2581 cgccccttac gcgcagataa agtccagcac gtccttcatg cttccctgtt tgttcatcac
     2641 gtcaaagctt gcgcattcgg tcttctgttc atagaccgta ttcatatttt cgcgaagcgt
     2701 tgtaatacgc tgcaccgcca catctaactg ccgggtgcca ttaatgggtt catagaaagc
     2761 cgccgccaga gaacgtggtt gttcggtgcc tttttctgcc agcgcccagg aggcgtaggc
     2821 acggctggca aagctgttct gtttgccggt tggggagact ttaagtgcgg cttccgtaaa
     2881 ggcgcgcaag gtctgattag ctaacgcttc gtcaccgccg aggttttcga ccagcagatc
     2941 tttatcgatg cagatatagg tgtagaacag cgcagaaccg aatccggttt caccaagatg
     3001 cccggcaccg gcatcttcag aagcctggcg caaatcatca acggcggtga aaaaatcatc
     3061 ttcgacaatc gtttcactga caccaaatgc atgcgcgacc tggcaggcgg cttcaacatt
     3121 aaattcgggt ttattcgcca gcatacgacc aaacatagcg atatctactg ccatgcgatc
     3181 tttacgtaac aaagcgagat cttcctcttt tggcgcgcgc ttttcttcgg ccagttgatg
     3241 ggccagcgct tttacggcgt caaattctgc cgggctgata tggactaatt gttcagtttc
     3301 ggcgttagtg agcggatctt ttggtttttt gtcgttttta gctttcccaa gataatccgc
     3361 aatttttgcc gcccattcga tggctttttt ctcttcgatg cctttctcaa tcaggatagt
     3421 tgccgcctca cgcgcaatac gcccactgcg aataccaata tggcccgcca gtgcctgttc
     3481 aaaaagtgca gaagtgcgcc acgcacgttt cagactttgc gaggaaacgc gcagtcgcgt
     3541 tgctccaccc aggaccacgg ttttcggcgc tccggtgtca tcacggttaa ggttggctgc
     3601 agggtaagcg gttaacaaat gaagctgaat aaatgtcgtc atgagatagt cctttattga
     3661 ttttgttcgt tatcgacgtc gccagcctgg taatattcca gcgcccagcg gatacggata
     3721 aattcggttg gccgctgatg acggcggtga tgattgagca gatcgtcgct ctcctggcac
     3781 cagcggaaga caccctctgc cagagagtca agattaacgg aaccgtttaa taatctgact
     3841 acgcggcgta actggcgcag taattcatcc ggtgttttta ctgccgacag gcgagtaaaa
     3901 cgcccctttg acattaccgc cgccagctgc gcagcaaaag gcagccgttc atcaatggct
     3961 ttaacattag cgctaagtgc ggctatcagc gccagtgcgg taatacgcca ttcaggttca
     4021 tcctgccact ttatttgtct gttcttcaga aacaggcgaa atccatccgt cagacaaaca
     4081 tcattaaccg tcgtactacg ccgcagactg gcacgttcgc cgcgtttctc ctgcaattcc
     4141 tcatgccatt tgcgcagcgt ggctttgtgc tcctctttta caatactcat tcagcagcct
     4201 cctgcttttt ctccctggcg gctttagcac tttgcttctc cgccgatgtt gtaaaatatt
     4261 tcttgcgcgc ggtcatgacg cgttccaaat caacgggctc ataaggattg gtgaatacac
     4321 gctcgtcaaa atcctgacgt gcgaataacc aaatttcctt ttgccatttg ccgagtaatt
     4381 catccgcatc ctgaccttct tcaatttggc gcactaacct caggaagcga tgctgagttt
     4441 tgttccagaa gtcgatatcc acaaaactga aatcacccct tgcacctttt ggatcggaga
     4501 accatgcttc tttcaatgca ctccgtaaca gactcagaat ccgtgaagcc gtttgcgcag
     4561 ccagccgcag cttcggtatc tggccttctt ttttattgag cagcagcggg aaatggtgtt
     4621 cgtaccaaca gcgcgctttc atgttgtcga aatcataacc aaatccccac aggcccactt
     4681 ttgcctgttt cagactgctg gcattaaaga gtttcaccac cagcgcggga agttccgtat
     4741 tgttttctga cttacccgtt tcgataaggc ctaaccagtc gcgccagatt aaaccgcccg
     4801 gttgtggttt aacggagtaa aactcaccgc cctctttaag tggtacacgg taaggcgtta
     4861 agggatgctg ccacatggca taattcgcac cgtaattttt ggtagtcatc aaactcagaa
     4921 gcgcgtcact ctgctcaccg caaatatcgc agttgccgac tgtcgtggta ttaaaatcaa
     4981 tacgaatacg ccgcggcatt ccccagtacg cctggagttt attgacctga tcatcggtta
     5041 ccaccgcacc ggccagttcg ctggtacgcg tcgggccaag ccaggggaaa accagatcgt
     5101 caaatttttt gggtagcggt aagtcggctt catcctgcgg catcacgttg agccacagtt
     5161 tgcgccacaa gggggcttgt tgattgccct gatactcctg caattcaatc agagtcgtca
     5221 tcggcccacc gccgcgtaaa ccggtgcgat agcctttgcc acctgacggc gcatttaact
     5281 gtagggagaa cagagctaac gcagaacaat gagagcatac gtgttcagtc acgccacgct
     5341 taataaagtg gtctttatta aacttcgttg tttgagcgcc gggaatctca ggcagtagcg
     5401 aagcgacctg aactttatcg cccatgagca cctcgaaatc ctgcataaat gaaggtgaat
     5461 ctgggccaaa ctggaaaggg tgttctaatg acagcaatgc ttcccgtagc ttttcagctt
     5521 ccagcccgtc ttcccagata tcatcccaac gacgataatc ttttggcgcg aaactgcttt
     5581 gtagtaaccc cagcaaaaac tgccatgccg ccccctggag atctgcccgc ggcgcagcga
     5641 tatcgacaac attttcatcc gccagatcga ctggcgccag cttgcctgtt gttccgtctt
     5701 taaaacgaac gggcaaccac ggggttgtca gaagtgaaaa cgagttcata tgtatcctct
     5761 cgtaaaaaag accatccctg gtcggttgtc catcattcca tccttttcgg tgtatcaact
     5821 attcagagag tcctgtcgca ggtatctcaa tcaaccttgc caatcaatcc ctccctggcc
     5881 gaatacccgc aactttcgtc atcagtgact aaaatcacgt ttgccatttc cggatcttgc
     5941 cgctgttcaa tgcaccactg cctgaacgct tccccttcca gtaaagaaaa ctcatcccga
     6001 tgttttttcc accagcttcg acgcactctg acaacgctca tttcccatgc gtgagcaccg
     6061 gtggcataag gcttcaccac accggcaata caggtagcca gccacaggga aacagattcc
     6121 tcagccagac gtgtcgacag cttttccgga aggtaatcgt tgatattggc ggcatagcca
     6181 ggcttgaagt tcaggacaaa ctttttagcc attgcgcgat cgcagtaata tttgcccact
     6241 tgctcctgct cgctgcgggc aaatccttcc ggcattacca cgtcctcacc gtagactgat
     6301 tcaataagaa ggcgggctgc gtgtggcatt tgaatagcgc cttgctcacg cagtacacgc
     6361 tgcgtcagcc agattcgtcc atgatcggga tagacatatg cactgttacg catggcactg
     6421 ccgaaccatt cgtcaccagg agcgtcgtcc cagacggggg ccagaatcag caattcagga
     6481 ggggaacgct cgtcttttcc gtcacgcttt aactgaccat taatatcgcg gatatgccgc
     6541 tgtaatcgcc ccgctcgctg aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg
     6601 tccaggtcac aatcaacgct ctgctctaag acctgagtac aaatgaggac ttttccggca
     6661 cgctgtgaac cgtcttcttt accaaagcgt gccagcgtct ccatttcaat tcgctggcga
     6721 tcgctaaaag caaagcggct atgaaagagt gaaaggctgg aagcgggaat gacgccgcgg
     6781 gcaagcagct gacgatgaac cttaatagcg tcatcgacag aattccggat ccaggcgatg
     6841 cattttccct gacttaccgc cgattcgata cgcgcaatac tctcttgttc actatgaagc
     6901 caacccacgc tgacgctacg ctcaacgtct ttgcgcgtcg ctacccggtg tgagttcaca
     6961 tcggatttcg tgacatgcgt cagccagggg taatcatcct tttcaaggaa cggagcttct
     7021 tgctggccct ctgtgccacg cgcaaaggcg gcgacgagtt tgtcgcgctg ctgttgggat
     7081 aacgtagcag aaagcaaaat gacgctgttt ccgccacgcg cctgccgctc gatcagccct
     7141 tcaagaatgc acgacatgta agcatcacag gcatggatct catcagccag caggattttg
     7201 ttactcaacc ccagaagccg cagattatta tgtttaaacg gcatcactgc catcatcgcc
     7261 tgatccagcg tgccgacgcc aatttcagcc agtagcgcct tcttgttact gttggcaaac
     7321 caggccgcac atccctgact gaatgtttgt tcatccggtt cttctgaccc gactaaatca
     7381 ccggaccaga gtgattcatt gaagcggtcc attaatgtgc gggcactgtg tgccagcacc
     7441 aagctggggc gggactctgg cgaatagaaa gcaagccagg ttttgaccag ccgatcgtac
     7501 atggcattgg ccgttgccat tgttggcagg ccaaaaaaca aaccctgtgc tttcctcgca
     7561 gccatcaacc tgtgcgccag gataagcgcc gcttctgttt tacctgcgcc agtcacgtct
     7621 tccagaataa ataactgtgg ccctggctgg ctgatatcca gatccagtac cttttgctgt
     7681 aatggtgtcg ggtgctcaat aaaaggaaac agcgtattaa ttccggtgaa aggtgcggtt
     7741 tctgcttttg gaggaaagac ggttaaggcg ttttgagcct gaactaaagt tttctgccag
     7801 taatctttaa tatccattgg gtgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc
     7861 cagtctgcga gtacgactgt tgcagagata taccaggaaa gttgttttaa aagttcaacg
     7921 ccctcgtcat catcccagaa tgtgggaatc tctatgagcg gaaacagtgc cttgatttca
     7981 aggagaaaat ctcgcgcggc agctttgtct tcaggcagaa aattatccag ctcatcaata
     8041 cggtcaggtg gtcgaccatg atgcccggta gttatggaca tccacatctc tattacacgt
     8101 gtaagtttac gagaagagag tgaagatgaa ggaagcaact cctcacattc acttaaataa
     8161 taattccaca gccagtaacc cagcgttgaa tgagagatct tttcgtaatt ctttctggaa
     8221 ccttccggaa tcttgagttc aggggccagg taaagttgct gaaaagagcg ggcaaatttt
     8281 ccaatatcgt gccagcacag caaccaagcg aaaaattgag ccgcctgttc cttgtcagaa
     8341 atccctaatt gacgaaagta atcagccagc ccgaagcaat ttcttttaac cattaaatag
     8401 cccattgcgg ccacatccag cgaatgccag caaagaaggt gatagccgtc gccaccctct
     8461 ttctcgccac gtcgggtttt tccccagaaa tcaaag
//
LOCUS       DAZKFZ010000195         8468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000195 DAZKFZ010000000
VERSION     DAZKFZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            55..315
                     /gene="dinJ"
                     /locus_tag="ACHOQW_003881"
     CDS             55..315
                     /gene="dinJ"
                     /locus_tag="ACHOQW_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP9574878.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            318..594
                     /gene="yafQ"
                     /locus_tag="ACHOQW_003882"
                     /pseudo
     CDS             318..594
                     /gene="yafQ"
                     /locus_tag="ACHOQW_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            750..1490
                     /gene="dpaA"
                     /locus_tag="ACHOQW_003883"
     CDS             750..1490
                     /gene="dpaA"
                     /locus_tag="ACHOQW_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP9574879.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1461..2228)
                     /gene="yafJ"
                     /locus_tag="ACHOQW_003884"
     CDS             complement(1461..2228)
                     /gene="yafJ"
                     /locus_tag="ACHOQW_003884"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP9574880.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2333..2911)
                     /gene="lpcA"
                     /locus_tag="ACHOQW_003885"
     CDS             complement(2333..2911)
                     /gene="lpcA"
                     /locus_tag="ACHOQW_003885"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP9574881.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3151..5595
                     /gene="fadE"
                     /locus_tag="ACHOQW_003886"
     CDS             3151..5595
                     /gene="fadE"
                     /locus_tag="ACHOQW_003886"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP9574882.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5638..6084)
                     /gene="ivy"
                     /locus_tag="ACHOQW_003887"
     CDS             complement(5638..6084)
                     /gene="ivy"
                     /locus_tag="ACHOQW_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP9574883.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6265..7035
                     /gene="yafV"
                     /locus_tag="ACHOQW_003888"
     CDS             6265..7035
                     /gene="yafV"
                     /locus_tag="ACHOQW_003888"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP9574884.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALITGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(7153..8325)
                     /locus_tag="ACHOQW_003889"
     CDS             complement(7153..8325)
                     /locus_tag="ACHOQW_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFP9574885.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNY
                     KGDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAE
                     GVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISG
                     IKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
     gene            8406..>8468
                     /locus_tag="ACHOQW_003890"
     CDS             8406..>8468
                     /locus_tag="ACHOQW_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574886.1"
                     /translation="MIYITIFMILPCPVPCSHVFL"
BASE COUNT         2061 a         2120 c         2080 g         2207 t
ORIGIN      
        1 ctcatttgag ctacaattca agctgaataa atatacagca taggagatat cccaatggct
       61 gctaacgcat ttgttcgcgc ccgaatcgat gaagatctga agaatcaggc ggcggacgta
      121 ctggccggga tggggctgac catctctgac ctggttcgca taaccctcac aaaggtcgcg
      181 cgtgaaaagg cattgccgtt tgatttacgc gagcctaatc aattaaccat tcaatcaatc
      241 aaaaacagcg aagctggcgt tgatgttcat aaggccaaag acgccgatga tttatttgat
      301 aaattaggag tttaaatatg attcaaaggg atattgaact cgggacaatt ttcaaaggat
      361 gtaaaacttg cacaaaagcg tcataaggat atgaataaat tgaaatatct tatgacgctt
      421 cttatcaata atactttacc gcttccagct gtttataaag accacccgct gcaaggttca
      481 tggaaaggtt atcgcgatgc tcatgtcgaa ccggactgga tcctgattta caaacttacc
      541 gataaacttt tacgatttga gagaactgga actcacgcgg cgctctttgg gtaaagcccg
      601 ctgccgatga aatttgtaaa ccctgcgccg acaggcttgc gtaaaactct tttggtgata
      661 atgtgagcct tcccaatcca gactattccg atttgggatc cattttgccg tcctggcgtg
      721 tgtaaccgtt ttatcaagga ataagcagta tgcgtaaaat cgcattaatt cttgcgatgt
      781 tgttgatccc gtgcgtttcg tttgccggtt tgctgggcag cagtagctcg accacgccag
      841 tgagcaaaga gtataagcag cagttgatgg gatcccctgt ctacatccag atcttcaagg
      901 aagaacgtac gctcgatctc tacgtcaaaa tgggcgagca atatcaactg ctcgacagct
      961 ataaaatctg taaatattcc ggcggcttag ggccaaaaca gcgtcagggc gatttcaaaa
     1021 gcccggaagg gttttatagc gtccagcgta atcagttaaa accagacagc cgttactaca
     1081 aagcgattaa tattggtttc cccaatgcct atgaccgtgc gcatggttat gaagggaaat
     1141 acctgatgat ccacggcgat tgtgtttcca tcggctgcta cgcgatgacc aatcagggca
     1201 ttgacgagat tttccagttc gttactggcg cactggtatt tggtcagccg agcgtgcaag
     1261 tgagcattta cccgttccgc atgaccgacg ccaatatgaa gcgccataaa tattccaact
     1321 ttaaggactt ctgggagcaa ctgaagccag gctacgacta ctttgagcaa acccgtaagc
     1381 caccgaccgt ttctgtggtc aatggccgtt acgtggtcag caagccgtta agccacgaag
     1441 ttgtgcaacc acaactggca tcaaactaca cgctccccga ggcaaaataa gcgccattcg
     1501 cctggcataa tcttttgcca ggtttcattg cccgtcagcg gctgtgtcgc aatcaccgtg
     1561 accacatcat tcggtgtggt ctgcgagctg aagtcgattt ccacatcctg atccagcaac
     1621 gttgccacgc caaacggcgc gcggcgggtg atccagtgca gattggtcga gcaatacgcc
     1681 attacatagc gcccatccga aagcagcatg ttgaaaacgc ccttctgccg cagttcatcc
     1741 gccagtgagg cgatatattt aaataccgcc gccatattgc ccggcgtacg cggataacgc
     1801 tgcgttaatt tatacagcag ccagcaaaag gctttttcgc tgtcggtttt acctaccggg
     1861 cggaagttgc cggtttccag tgatttatag cccgtcagtt gtccgttatg ggcataagtc
     1921 cagttacgtc cccataactc gcgggtaaac gggtgagtat tttccaacgc cacctcgccc
     1981 cgatttgcct ggcgaatatg agccacaacc gaacaggatt ttatcgggta gtcctgaaca
     2041 agtttggcaa tgggggaatt aaagctgggt tgtggatctt taaatgtgcg acagccttta
     2101 ccttcgtaaa aggtaatgcc ccagccatct ttatgtggcc cggttccacc accacgctgc
     2161 accagcccgg tgaaactaaa gcaaatatcg gtagggacgt tggcgctcat cccgagcaat
     2221 tcgcacatcg gtaaccccca caacagcggg agcgcatggc ccccggcgaa gattgactta
     2281 agaattttca ggccggatgc ggcgtaaacg cattattcgg cctacgccag acttacttaa
     2341 ccatctcttt ttcaattaac tggatcagga tatggatcac tttaatgtga atttcctgaa
     2401 tgcggtcggc ataaccaaaa tgtggaacgc gaatttcaat atccgccgta ccagccattt
     2461 tgccgccatc tttgccggtc agggtaatca ctttcattcc cttctcacgc gccgccgcga
     2521 tcgctttgat cacgtttgca gagttaccag acgtggagat ccccagcagt acatcgcctt
     2581 cgcggcctac cgcttcaaca tagcgggaga aaatatcatt gaaaccaaaa tcgttaccga
     2641 cgcaggaaat atggctgacg tcagagatgg caatcgccgg gtagcccgga cggttttcac
     2701 ggtagcgacc ggtcagctct tcggcaaagt gcatggcgtc gcaatgggag ccgccgttgc
     2761 cgcaggaaag caccttgcca ccggctttaa agctgtctgc taacaggacc gccgctcgct
     2821 gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc gccgcttcgt
     2881 tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata aatgtaatag
     2941 acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt gctatgacca
     3001 ggactttttg ccctgaagtg tagataaaaa cagcaacaat gtgagcttcg ttgtaattat
     3061 attgtaaaca cattgctaaa tgtttttaca tccactacaa ccatatcatc acaagtggtc
     3121 agacctccta caagtaaggg gcttttcgtt atgatgattt tgagtattct cgctacagtt
     3181 gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag tctgattttg
     3241 ctcgcctgga cagctgccct cggcgtcgct ggtctgtggt cggcgtgggt actggtgcct
     3301 ctggccatta tcctcgtgcc gtttaacttt gcacctatgc gtaagtcgat gatttccgcg
     3361 ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg
     3421 attgatgcgg gtaccacctg gtgggaaggc gacttgttcc agggcaagcc ggactggaaa
     3481 aagctgcata actatccgca gccgcgcctg accgcagaag agcaagcgtt tctcgacggc
     3541 ccggtagaag aagcctgccg gatggcgaat gatttccaga tcacccatga actggcggat
     3601 ctgccgccgg agttgtgggc gtatctgaaa gagcatcgct tcttcgcgat gatcatcaaa
     3661 aaagagtacg gcggcctgga gttttcagct tatgcccagt ctcgtgtgct gcaaaaactc
     3721 tccggcgtca gcgggatcct ggcgattacc gtcggcgtgc cgaactcatt aggccccggc
     3781 gagctgctgc aacattacgg cactgacgag cagaaagatc actatctgcc gcgtctggcg
     3841 cgtggtcagg agatcccttg ctttgcactg accagcccgg aagcgggttc cgatgcgggc
     3901 gcgattccgg acaccgggat tgtctgcatg ggcgaatggc agggccagca ggtgctgggg
     3961 atgcgtctga cctggaacaa acgctacatt acgctggcac cgattgcgac tgtgcttggg
     4021 ctggcgttta aactctccga cccggagaaa ttactcggcg gtgcagaaga tttaggtatt
     4081 acctgtgcgc tgatcccgac caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg
     4141 ctgaatgtgc cgttccagaa cggaccgacg ctcggtaaag atgtcttcgt accgatcgac
     4201 tacattatcg gcgggccgaa aatggccggg caaggctggc ggatgctggt ggagtgcctc
     4261 tcggtaggcc gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa atcggtagcg
     4321 ctggcaacgg gcgcgtatgc tcacattcgc cgtcagttca aaatctctat cggtaagatg
     4381 gaagggattg aagagccgct ggcgcgtatt gccgggaacg cttatgtaat ggacgctgcg
     4441 gcatcgctga ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc
     4501 gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat ggatattacc
     4561 ggcggtaaag gcattatgct cgggcaaagc aacttcctgg cgcgtgctta ccagggcgca
     4621 ccgattgcca tcaccgttga aggggctaac attctgaccc gcagcatgat gatcttcgga
     4681 caaggagcga ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc gaagaacaat
     4741 gacgtcaacg tgttcgataa actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa
     4801 gttcgcagct tctggctggg cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat
     4861 gccactaaac gctactatca gcacctgaac cgcctgagcg ccaacctcgc cctgctttct
     4921 gatgtctcga tggcagtgct gggcggcagc ctgaaacgtc gtgagcgtat ctcggcacgt
     4981 ctgggtgata ttttaagcca gctttacctc gcctctgccg ttctgaagcg ttatgacgac
     5041 gaaggccgta atgaagccga tctgccgctg gtgcactggg gcgtacagga tgcgctgtat
     5101 caggcagaac aggcgatgga cgatttactg caaaacttcc cgaaccgcgt ggttgccggg
     5161 ctgctgaatg tggtgatctt cccgaccgga cgtcattatc tggcaccgtc tgacaagctg
     5221 gatcataaag tggcgaagat tttacaagtg ccgaacgcca ctcgctcacg tattggtcgc
     5281 ggtcagtacc tgacgccaag cgagcataac ccggttggtt tactggaaga ggcgttggtg
     5341 gatgtgattg ccgccgaccc gattcatcag cggatctgta aagagctcgg taaaaatctg
     5401 ccatttaccc gtctggatga actggcgcac aacgcgctgg cgaaggggct gattgataaa
     5461 gatgaagccg ctattctggt gaaagcggaa gaaagccgtc tgcgcagtat taacgttgat
     5521 gactttgatc cggaagagct ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa
     5581 gttgaagccg cgtaattgaa taacggagcc gaaaggctcc gtttctttat ccgctaatta
     5641 tttaaaatta aagccatccg gatggttttc caggctgccg gtcaacgccg cgaacagcac
     5701 cgttttacca tcaatcgaaa gcgcatcgtt cacattcaac caggtcagtt tctcttgcga
     5761 cgttttctca tcaatagtcg agaacagccc cgtcatctga ttagatttct cggaccacat
     5821 cacagcgata cgttgcgagc cacagtcatg cggtttgcac gcgctcatca cctgatacgt
     5881 ctcatctccc aacgttacgg tttgtgcagg ggtataagta ccgcctttca tcacccaggc
     5941 aggcagctta tgcccttgca ccatctgatt aaatgcagct ttggtggttt cgccctttgc
     6001 aaggctgcta atggttaaat catcctgcgc cattgcactg gtggcgatga ccagtgcggc
     6061 gactgtcgtt attgccttaa acatcattcc tcccgagctt atcctgccca tgttattaac
     6121 ctcctaaagc ctggcgataa cacgataatt tgcaaatcag atatttccaa tatcagcatt
     6181 tgttatcaat ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc
     6241 cttcgcataa aggagttgta caccgtgcct ggtttgaaga ttacgctttt gcagcaacca
     6301 ctggtgtgga tggatggtcc tgccaacctg cgtcattttg atcgtcaact ggaaggtatt
     6361 accgggcgcg atgtgatcgt tctaccggag atgtttacca gcggctttgc catggaagcg
     6421 gctgcttcgt cgctagcaca agatgacgta gtgaactgga tgacagccaa agcgcagcag
     6481 tgcaatgcgc tgattacagg cagtgttgca ttacaaacgg agtctggttc ggttaaccgc
     6541 tttttgctgg ttgagccggg cggcacggta catttttatg ataagcgtca tctgttccgc
     6601 atggcagatg agcatctgca ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc
     6661 ggctggcgta ttttgccgtt ggtgtgctac gacttacgtt ttccggtgtg gtcgcgcaat
     6721 ctcaacgatt atgacctcgc catctacgtt gccaactggc ctgctccgcg ctctctgcac
     6781 tggcaggcat tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat
     6841 cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg acagccgggt gattaatcca
     6901 caaggcgaga ttatcgctac tgccgacgcg catcaggcaa cgcgcattga tgcggagctg
     6961 tcgatggtgg cattgcggga atatagagaa aagtttccgg catggcggga tgcggatgag
     7021 tttaggttgc ggtgagtggg ggctgtattt agggcgggga attcagaaag ggatgtttag
     7081 tgtgctgagc gagagttaaa taataaaaaa ctgggaagat ttaataatcc ccccgcaggg
     7141 caagagcatg aattaaatct gtgttaaatt tttcattttg tcagttcgaa acccaagctc
     7201 tgagcaggat gcaagcactt cttctatata acatgtgttt aacgcaacct tctttctttt
     7261 cctcttcacc ccacccttaa tgtctttgca atctcttaat aaattcagtg ccatcttctt
     7321 tattccagat attatttcgg ctgcgtttcc tcttcttatc cggctggcat cttcattcat
     7381 ttttacatct aacacccaat gaagactgtg ctcgatcagc cagtgcgctc tgatagcatg
     7441 tgcaaattct ttagcatcca tatcctttga tgaaatataa tatcggatgc ttacaccttc
     7501 tgctgattta tcttctttct tctgcctgaa tgacaatgct acacaaagct tttttaatcc
     7561 cttccattcg aattcaaaat cacaaaattc aggcgttacg ttactgacaa tatgcaaacg
     7621 tgtttctttt cttccatgac ttatctcctg cgtactaaac gaatcgcctt tataattaga
     7681 aaacacattt acaggaaatt tttcctcgaa tgcatgatgt aatttcccct gattgccttt
     7741 tactgccaga agataatctg cttttttatc tttgatcttc gaagcgatat ctttctgaca
     7801 gcccatagca tcaatggtta tcaaattttt ctttaaatcc agtaggttaa gcaactctgg
     7861 aatggctgta atctcattac ttttggcttc cgtttttacc tgccccagta caacaccatt
     7921 ttcgttcgag aatgcactca ccatatggat tgctcctttt ctttttccct tatcaaagga
     7981 gcctcttatg gtctttccat ctattgctat aatttcacca tcagtgattt catggcactc
     8041 ctgcatccat tcaataaata tcttttcaaa ggccaaactg tcaatgttac tcacaacgcg
     8101 tgcaatggtg tcatcgaccg gaatgccatt atcaaaatca ccatatttct ttagccattc
     8161 aagtctttca tgtccaaaat cttcaatttc ctgccactca tcggcacctg caattactgc
     8221 acatacggtg agaaacaaaa tatcagataa tttatgttta acctttcctt gttgtcgtat
     8281 atcagggatc actgaaatat aatcaagcaa actttgaata ctcacctggc gtccttttta
     8341 tctttttagt tattccaaag gttaaatttt gatcacctgt aggcgttaaa gccaggatga
     8401 aaatcgtgat ttacatcacg attttcatga tcttgccctg ccccgtccct tgctctcatg
     8461 tctttctg
//
LOCUS       DAZKFZ010000196         8342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000196 DAZKFZ010000000
VERSION     DAZKFZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(339..414)
                     /locus_tag="ACHOQW_003891"
     tRNA            complement(339..414)
                     /locus_tag="ACHOQW_003891"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(379..381),aa:Asn,seq:gtt)
     gene            complement(515..2002)
                     /gene="yeeO"
                     /locus_tag="ACHOQW_003892"
     CDS             complement(515..2002)
                     /gene="yeeO"
                     /locus_tag="ACHOQW_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9574887.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            2163..2238
                     /locus_tag="ACHOQW_003893"
     tRNA            2163..2238
                     /locus_tag="ACHOQW_003893"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2196..2198,aa:Asn,seq:gtt)
     gene            complement(2276..3226)
                     /gene="cbl"
                     /locus_tag="ACHOQW_003894"
     CDS             complement(2276..3226)
                     /gene="cbl"
                     /locus_tag="ACHOQW_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP9574888.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3328..4245)
                     /gene="nac"
                     /locus_tag="ACHOQW_003895"
     CDS             complement(3328..4245)
                     /gene="nac"
                     /locus_tag="ACHOQW_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP9574889.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4571..4646
                     /locus_tag="ACHOQW_003896"
     tRNA            4571..4646
                     /locus_tag="ACHOQW_003896"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4604..4606,aa:Asn,seq:gtt)
     gene            complement(4702..5637)
                     /gene="ldtA"
                     /locus_tag="ACHOQW_003897"
     CDS             complement(4702..5637)
                     /gene="ldtA"
                     /locus_tag="ACHOQW_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9574890.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5699..6778)
                     /gene="cobT"
                     /locus_tag="ACHOQW_003898"
     CDS             complement(5699..6778)
                     /gene="cobT"
                     /locus_tag="ACHOQW_003898"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP9574891.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(6790..7533)
                     /gene="cobS"
                     /locus_tag="ACHOQW_003899"
     CDS             complement(6790..7533)
                     /gene="cobS"
                     /locus_tag="ACHOQW_003899"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP9574892.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7530..8075)
                     /gene="cobU"
                     /locus_tag="ACHOQW_003900"
     CDS             complement(7530..8075)
                     /gene="cobU"
                     /locus_tag="ACHOQW_003900"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP9574893.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8227..>8342)
                     /locus_tag="ACHOQW_003901"
     CDS             complement(8227..>8342)
                     /locus_tag="ACHOQW_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP9574894.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         2313 a         2246 c         1800 g         1983 t
ORIGIN      
        1 ggagaaataa tatttattaa aattccaggt cttctttttc tgattaccga agacaaagtg
       61 gcaattacgt atagtttccc gataaagacg cgacagcgac atcccgcata aggcattttt
      121 tctttatctt tgtacggtac ttcatggaac agagtttttg accttgcgaa tcgtgatgtc
      181 tgttgggggc aatttactca ctgaagcgtg agactcgatt aagcgcacga aacacagaaa
      241 tcaaaaaacc cggtcacttt ttacaaggca accgggtaaa aataattttt attttttaac
      301 tgccctgaga ctcataaaga tgcctcaaaa ctaaaatttg gctcctctga ctggactcga
      361 accagtgaca tacggattaa cagtccgccg ttctaccgac tgaactacag aggaatcgtg
      421 agaacgaggc gaatattagc gatgcccacc cacaatgtca aagcctgttt tttaaatttg
      481 aaatcgtttg ctgaaataat ctgcattttg tcgtttattc cgacacagct ggcttttttt
      541 cacacttttg cggttcgggt cgagggtatt tccacagcca acgtccagta accattcgcc
      601 agtaaaacag cacggcccgc acagcccagt cggcaaacat tcccatccag acaccaacca
      661 caccccagcc aagcatgatg cccagcacat aaccgacgac aacccgacaa ccccacatgc
      721 tcaacataga aacccacata gcgtaacggg cgtcacgagc ccccttaaat ccagcgggta
      781 gcacccatga agcggaccaa ataggcataa ataaagcatt tagccaaatc agaatcacaa
      841 cgacatgttt aacctgtgga tcctgggtgt aaaacgatgc cataaccccg gcaaagggag
      901 ccgttagcca ggcgatggcc gttaatccaa gagtggtcag ccaaaacaca tgccgcaact
      961 gaatctctgc ttgcgctatc tgccctaccc ccaaccttcg gcctgtaatg atcgtagaag
     1021 cagaaccgag cgcacttccg ggtaagttga taagagccgc aattgaaaac gcgataaaat
     1081 ttccggcaat aacactggtc cccattccgg caacgaacat ttgggttaat aaccgaccac
     1141 tggtaaataa cactgattcg acactcgctg gaataccaat ccccatgact tcccagataa
     1201 tgctaaaatt cagcggttta aaatagctct ttaacgaaat ccttagcgca ggattaaaac
     1261 caatcgccag cacccacaaa attgcaactg cgccaatata acgagaaatg gttaaaccca
     1321 acccagcccc gacaaatccc agtcccggcc aggagaaaag gccgtaaatc aatatgccgc
     1381 taataataat attaagaata ttcaggctac cgttaatcag tagcggtatt ttcgtattcc
     1441 ctgcaccacg aagtgccccg ctaccaatta gagtgatggc agctgctgga taactgagta
     1501 ccgtcagctc cagataagtc aacgccagtg ctttaacttc tgtcgtggca tcacccgcga
     1561 cgaaatcaat aatttgctcg ccaaaatgat gaataagcgt tgccaacagt acggcaaaca
     1621 acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc tcgtcgtcga tcccgcttac
     1681 cgagactaaa tgccacaacg acagtagtac caagatcgat agcagcaaaa aaagccataa
     1741 tgaccatatt gaagctgtcc gccaatccca cgccggccat cgcatctttt cccagccagc
     1801 tgaccagaaa agtgctcaga acccccatca acaggacaca ggcattctcc atgaagatag
     1861 gaacagcaaa cggggttatc tcgcgccaga acaacacttt gtagctcttg cgtttagcgt
     1921 gccagcgagt gccgtgaaca acctggcgta aagcagagga gatattcaaa gccgacctta
     1981 attgcagaaa gtgaaaccac atttcaaata atgagggaga atcagcaaag ctgcaaagat
     2041 tttcgccatc aaattgtctg caaatgcaac aaactgttga tagaaacggc aaacagttgg
     2101 gaaaattaaa aatcgggttt gacaaaagat ttttcgccgt taagatgtgc ctcaacagcg
     2161 attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga
     2221 gtccagtcag aggagccaaa tttctgaaaa gcccgctttt atagcgggct ttttgctata
     2281 tctgataatc aatttcctct tcactgtttt ccatcacctg ccgcttgata tcctcaactg
     2341 aaagtcctgc attgcaaagt tccagaaagc gccagacata gttacgctga agttgtcctc
     2401 gcttcagtcc caaccagaca gtattagcat cgaaaagatg ccgcgtatcc aggcggatta
     2461 aattcttttc ctcttgttca ccgctggatt gttcggcaac taatccgatc ccaagcccaa
     2521 gagcaacata ggttttaatg acatcagaat cctgcgcact taatataata tctgccagca
     2581 aacctttgcg ggcaaatgcg tcatcaatac gtgagcgccc cgtaatcccc tgtcggtaag
     2641 tgattaacgg ccacttcgct attgattcca gcgtcaatgg tgtaatttgc gtcaagggat
     2701 gatcgtgtgg aacaagcaaa ctatggtgcc aacgaaacca cgggaaggcg acgagctgcg
     2761 ggtcattact caaacgctcg ctggcgatac caatatcagc ttcaccatta tgcaacaacg
     2821 tcgcaatttc ctgtggcgtc ccctggatta gctcgagccg aacctccggg aaaagttcgc
     2881 gaaaagcttt aatgacctct ggcaagctat aacgtgcctg agtatgcgtc gttgcaatag
     2941 tgagaacgcc agacgtatcg ttggtaaaca ggtctgcaag ccgacgaaca ttactggctt
     3001 cattgagaat acgttctgca atgaccagta atgctttgcc cggttcagtc atgcccagca
     3061 gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc atcctccagt tcccgaatat
     3121 gacggctgac gcctgactgt gaggtaaaaa gcatattcgc aacctctgtc aggttgtaat
     3181 cctgacgtgc agcttcgcgg attatcttta gttgttggaa attcacggta aactccgggc
     3241 agttcagata tcccgttatt gttaaagtct aacgcccggc ataacaaata ataaaaaccc
     3301 gcatcttatt ccatcccgat ataacgctta gctcaccaat tgccactgcc ttttttccat
     3361 cactggagaa ctaatcactg acattaacaa ctctttcact gcctgtgcct gtggcgataa
     3421 gttcgctctg gcgggtaaat ttaatgacaa agagagactc atggaaggcg tggtaatgcg
     3481 tgacatccac ccatttactg cgccacataa cgaacgcgcg gccgattcgg gtaatactgc
     3541 aacgcccatg ccgctggcaa tcgctgcggt aagcgtagca atagactcaa tttcaccaat
     3601 gacttttgcc gtgagccgcc gtagggaaaa cgcctcatca acacgaagtc taacagcgct
     3661 gtaatcactg gggagaaaaa ggttcatttg tgcaatagca ttaacatcaa cgctttgccc
     3721 cgggcaatct tgagttccta ccaggaaaag atcttctttc agcaaagcct gactggatac
     3781 accagccaca ggggaatgct cataaatcac cgccatatcg agttggtgat ttatcagttt
     3841 ttcgttaagc actgcaccgc tattttcatg aagatagata acgacctccg ggaattcagc
     3901 gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca gcggttcctg gcgcaaagcc
     3961 aatcgagact tgccccgata atgcctgacc aacgttatgc accgccagtt gggcctgctc
     4021 acactgacgt aaaatggccc gcgcatgggt atagagaatt tttccggcgt ctgttggcgt
     4081 aacgccccgt tttgtgcgaa tcaaaagttg ttgatttaac tcaccttcga gtgtggcaac
     4141 ctgctggctg agcgctggtt gtgcgatatg caatacttca gcagcctgag tcaggctacc
     4201 aatatctaca atttttacga agtatttcag gcgtctgaag ttcatgttgc ctccggtttt
     4261 tgagaatcgg cccaagtgcc gccattactt acaaccagat tgcaagatgc ttgccagttt
     4321 tattttggcg ttgatgtaca agctaaccaa ctgtcaaata agagattatg atagattcgt
     4381 cattggctct tttcaccagc agtcgcgttc ccctgcccta taaaaggagg gtatgcacca
     4441 cgatggttca ttaccaataa gattgaaatc tcatcacttt gttgaaattg acagcaaaca
     4501 aacaaaaaaa tgcatttcac cctttgacat caccatgcac tgccattaat atgcgccccg
     4561 ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
     4621 tggttcgagt ccagtcagag gagccaaatt caaaaaagcc tgctttctag caggcttttt
     4681 gctttctaat taccaacgct cttaaaacat ctgtcttgaa ccagaactaa tttgcacagg
     4741 cattcccgat cgacgttgca acgcagcatt tgcgcgattc acatcaactt cttgcccgtt
     4801 gataaacgcc cgcaaagatg gggttaccgg caatggcact tttcggtcag actcatattc
     4861 agcacgattg cgcgacagcg gctcatgaac ttccagccac tttgaaccat ccggttccgt
     4921 tgtgtatttc actggctgat caataatttg cacacgagta cctacaggaa cattatcaaa
     4981 cagatatttg atatcgtcat tgcgcagacg aatacagccc tgacttaccc ggagcccaat
     5041 accaaaattg gcattggtac cgtggatggc atatagcctg ccaatataaa tcgcgtacag
     5101 ccccatggga ttatcgggcc ccgcaggaac aaatgcgggc aaactctccc ctcgtttcgc
     5161 atattcgcgc cgggtgttcg gcgttggcgt ccaggttggt gcttcttgtt tacgttcaac
     5221 ggtagtcacc cagttacgcg gggtttctcg cccagcctgg ccgataccaa taggaaagac
     5281 ttccacagta ttactgtctg gtgggtagta ataaagacgc atctcagcga cgttaaccac
     5341 aatcccttta cgaacagtga cgggcaaaat cagttgctgc ggaatggtga gttgcgaacc
     5401 tgacttcggt aagaagacat cagcgcccgg attcgcttcc agcatgttac ttaacccttg
     5461 cccgtattgt gcggcaaaag tctccagcgg ctgggtattg tgatcaggaa cagttacagt
     5521 aagcgactgc cccactaaac ggctaccctc tggaggtaat ggataagtta ccgccaggct
     5581 agtatggctg gcaaaaagca gagcaaatga gcaaagaata tttacacgac gcatcatgtc
     5641 cctttcctaa gtcgcgaaag ctatcggtta agtatagctt ttatcagact tttcgttttt
     5701 aactgttcaa atcagaagtc gtattccccg gtagaacaat tttactggca gcaagttcgc
     5761 ccatgttgtt gtatatcgca caagcagctt cgatgatggg catcgccaga gctgcgccac
     5821 tcccctcacc taaacgcata tccatattga gaaaaggctc cagccccaaa tgcgacagcg
     5881 ctatacgcgc ccctttttct gccgacaggt gagaaggaat gagatacggt ttgattgcag
     5941 gagacatctg gcaggctgcg agcgcagcag cataagaaag aaatccatcc agcaacacgg
     6001 gtaaaccgca ggaagcagcg cctaacatca cgccagccat tccgaccaaa tcaaatccac
     6061 ccacttttgc cagaacatta acgccatcct gcggatttgg ctgattcaac gtaatcgccc
     6121 ggcgcacaac atcaatttta ttagccagtt tatctgtcgg caggtttgcg ccaatcccaa
     6181 ccacttcttc aggatcccgg ccagtgattg tgctgactat tgctgctgcc ggtgtcgtgt
     6241 ttgccatccc cagttcacct acaccaaaca gcgtgacacc gttttttgcc agctcccgcg
     6301 tataacatat gacgtccaaa agtaactttt cagcctgacg gcgactcatt gccggagctg
     6361 aagcaatatt gccgctacct cgtgcgacac gcatgttgat aagcccgggg ataggctcag
     6421 cagtatcaat accaacatca attacgtgga cgttagcgcc cgcttgtgct gccagcacac
     6481 acacgccggt tgttccgcgg gtcatatttt cagcctgaat ggctgtcact tcttttgggg
     6541 aaatagcgac cccttcctcc cagacgccgt gatcggcaca cataaccagt accgcttttt
     6601 tgcccacatg cggtatgcca ttcaaccccg gcattcccgc cagttgtacg ccaagcgcct
     6661 ccagccttcc caggctacca acaggtttga gtaacccgtc aatatgccgt tgtgcacgcg
     6721 acatagcggc aggatcgatg gcagggatcg tattcagtaa attggcaagt gtttgcatgt
     6781 cacgtctctt tatagcagtg ccagcaggaa gaccagttca ccaagctcta tggctgctcc
     6841 cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga agctgaccga ggatgaaaat
     6901 cgccaccatc gtcaccacca tagcagccac accatgcata cccggcaata atacggcagc
     6961 aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc ccatcaattt tgccaatgaa
     7021 cacattgcca agcccttctt cccgggcata acgatgacga tacatcaata atgcggcagt
     7081 cccgcggctg accgcacatg ctgccgccag cgatgcaagg atcggttcgc cacgcagagc
     7141 caactcgctt aacaccaaaa tctttgccag tacaacaaaa atcaatgcca gaccgccgtg
     7201 ggtacctaaa cggctatcac gcatgatttc caacatgcga tcgcggctac gtgcagaaaa
     7261 tacgccatca caggtatcag cgaggccgtc cagatggaat cccccggtca tcagcaccag
     7321 cacaagtacg ctaaacagtg ccgccagtgg tgcgccacac catgcctgca gcaccatgaa
     7381 gaccagcccg ctaatcgcgc caagcaataa tccaatcaaa ggaaaagtaa taataccgcg
     7441 agaataatgc tcgaaatcca gtccctggga ccagcgacgc ggtacaggca gacgcgtaat
     7501 aaatgagagc atcgcccaaa ataatttact catttgattt ttactccaat acccgaaacc
     7561 accagccata cttcatttgc cgcagcggcc aactgctgat ttacccgccc ggcaatatca
     7621 cgaaaatgtc gtgccagacg actctccggc acaatcccca ttcccacttc gttagtcact
     7681 aatacaacct ttgcggggca acgttggcag gcagcaatca acgactgaat ctcagcatta
     7741 atcgcctgtt ccatcgcctg ataatcccat tcatcagggt ctttatcgcc gccataatca
     7801 aacaacagat tagtcaccat tgtggtaacg cattcaagca acacaacctc attcgggtta
     7861 atgtctgcat gaattaattc atcaagatgt tgccagcgct ccactgtgcg ccagtgctcc
     7921 gggcggcctt gccgatgatg ttctatccgt gcagccatct catcatcaag gatttgcgag
     7981 gtagcgatat acagaacctg tgaagagtcc ccaataagcg cctctgcgtg gcgactcttc
     8041 ccgctccgtg cgccccccgt caccaaaatc atcataccga ctcctgattc aaatgtatga
     8101 tggtattcat tgaacagaac aatggatccc ccaccgttgc tgaccgcaaa acatttacgc
     8161 gattaatact gcgcgtaata taattaaaaa tccagcattc tcaatccatt acgaaataag
     8221 atggcattac ggaatagcca gactttctcg tgttaacgtc cgacaaaatg cgtcattgac
     8281 agcggtttct gtggattgac acatttcatt cagataatga attaatgcta ctgccggaac
     8341 aa
//
LOCUS       DAZKFZ010000197         8329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000197 DAZKFZ010000000
VERSION     DAZKFZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(8..2854)
                     /locus_tag="ACHOQW_003902"
     CDS             complement(8..2854)
                     /locus_tag="ACHOQW_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFP9574895.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAIALSLAA
                     VTSVPALAADTVVQAGETVSGGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAVEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLESHSAQNTLVDDGGILAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGN
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGRGWLGSLETGLPFSITDNLMLEPRL
                     QYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSINGSSAEGYNSQATLNVTF"
     gene            complement(3226..4098)
                     /locus_tag="ACHOQW_003903"
     CDS             complement(3226..4098)
                     /locus_tag="ACHOQW_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9574896.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            5439..6929
                     /locus_tag="ACHOQW_003904"
     CDS             5439..6929
                     /locus_tag="ACHOQW_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9574897.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTSWKNKEEILKAQKKALLSKLNKELRKGADESETLRQLEALQKNREEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLDKLNACYSQKLIRWHEQSFGL"
     gene            complement(7051..7623)
                     /locus_tag="ACHOQW_003905"
     CDS             complement(7051..7623)
                     /locus_tag="ACHOQW_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574898.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(7709..7915)
                     /locus_tag="ACHOQW_003906"
     CDS             complement(7709..7915)
                     /locus_tag="ACHOQW_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9574899.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
BASE COUNT         1973 a         2076 c         2154 g         2126 t
ORIGIN      
        1 agaattatca gaaggtcaca ttcagcgtgg cctgactgtt ataaccttca gcgctgctgc
       61 cgttgatgct gtgggcataa ccggcctgaa cgcccagggt gatattttcc cggacacggg
      121 cttccagtcc ggcctgcaga tccagtgaag tgccattctg tgagggagag aacgtcatcc
      181 cgctgcctgc ggtggaggtc cccacacgca tatcaccccg ggagctgaag gtgcggataa
      241 cggaaggctg tacccaccag ttcaccggca gttcacgcac actgtgtttt gcactgtcgc
      301 gcagggtgtc acgggatgag gtgccttcac caaaggtcat atcgttgtgg ctgcccaggc
      361 ggaaaccggc acgcacatgt tgtgcactgc catgcccgaa cttcacataa ccggcgttgt
      421 ccttaccgtc atccagggag agcccctgcc aggtgtactg cagtcgtggc tccagcatca
      481 ggttgtcagt gatactgaag ggcagaccgg tttccagtga acccagccag ccccggcccc
      541 gggcgcggaa gtcgttattg tccgatgatg ctttcatgct gtggcgggtt ccctgtgcca
      601 caatgtcagc ccacaggccg gaggatgtat gtgtcagatt caggtatccg cccaggctgc
      661 cggcatcatc ccggaccgtg ccggcgcggg agccgtcatc atccttaaca tcaacggaag
      721 aatggcctgc agcaccatac actccagtcg tcagtgacat accggcgacc tctgttctga
      781 gcaggtcacc ctccagacgg acgaagccat agctgccgct gctttccggc gtggctccac
      841 gggcaatacc gccgttgtta tcgtgaccga gatgaccgcc ctgaatgctg agacggacgc
      901 tgttattttc accgtttaca ccggtctgat ggctgcggga gcctgccaga atccggtcat
      961 agtccattgc ctgtgtcagc atggatgtat acagggggac ttcagcacga taagcatttt
     1021 cactgcgcag gtaccagtct tcatcgctgt cacggttcag ggtgtagtta aaggcgccgg
     1081 cctgaagcgg gcgactcagg gcaaacgcac cttcttctgt ggtggcgcca ttctgtgcat
     1141 ccacaacccg gataccctgt ccggaggttg ccaccccgag gttactgttt ccgacatttg
     1201 taaacgcaag ccaggttttg ccggttgcct gaccaccatt aatcaccagc tggtcagagg
     1261 tattgctgcc atcaaggcga acacgcatat tgatggtgcc accctgaccg gtgaggttac
     1321 tggtggtcag tttatggaac gttaccgggg agtcgccttt tgcaacagca cggctcagcg
     1381 cggcatccgg tgtcgtctga ttatcaaagc gaatctcccc tgcgttaaca atatcgccgg
     1441 tactgaccac atcaccattc agtaccatgc tggcaccttt actgagctgt gttctgccac
     1501 tcagatttcc cccggcagcc agcgtcagtg ttccacgatg tccgacagtg gtgttgccag
     1561 cctgtcctcc ggcattaacc gtaaagctgc cgccattttc cagcagcagg ccgctggcct
     1621 gaccggagat accatcaata ctgaacttac cgctggcatt ggtcccctca acagtggcac
     1681 cactgtctgc tatcagggca ccaccggatg tcatggtgac atctgttgcc ttaccaccgg
     1741 cagacactgc caggataccg ccgtcatcca ccagggtatt ctgtgctgaa tggctctcca
     1801 gtacatccag acgaccgccg gattccagaa caacaccgtc agccttaccg ttttccacag
     1861 tgaaattgcc cagacggttg ctgccggtga cggttgccgc cgtactggtg accagtgcgc
     1921 ctccctgctt cagggtgaca ttcgtggctg taccaccggc gttcacctgc agtttgcctt
     1981 tctggttaac ggtggtgaaa tccgccagac caccttcctt gacaatctgc cagccgtcac
     2041 tgtttacaac cgtgtcagag gctgtgcctc cgttgtgcac atactggtaa ccgccattca
     2101 gtgtggtatc cagcgcatgc ccgtgtaccg tctggtcgcc gccggcataa accacagtgg
     2161 tatttgctgt tccttcaaca gccacaatct gacgaccatt tttattgatg gtggtacgta
     2221 cggcatttcc gcgaacaaac tgcccggtat caccattttc tgcatctggt cccccttccg
     2281 cgccggtatt cacaaccgtg tcggttgcca ttgcgccgga tttgacggcc tgccagccct
     2341 tctcattaat gacggtaccc gttgcaatcc cgccttcatg tacccactgc tcaccgccgt
     2401 tcagagtggt attcactgcc tgcccctgaa ggctctgtcc gcctccggca ctgataaccg
     2461 tgtctgaaac gcttcctccg gcattcactc tctgaagacc accaccggtg acagtagtgt
     2521 tgttggcgat accgccattt tgtatccatt gcccgccggt attggcctcg ttatccggcc
     2581 catactccag accggtactg atggtcattc cgttggccgt accgagaaca atctggttgt
     2641 catgatttgt cagtgttccg ccgctcacgg tttctcccgc ctgtacaacc gtgtcagcag
     2701 ccagtgccgg gactgatgtg acagcagcaa gagacagcgc aatcgccaca ccggcgcgtt
     2761 ttccccgtga gcgggccagt tcggaggcca ccaccagggt acccgtaatg tgattccata
     2821 ccagcctgta gctggtgttc agatgtcgtt tcatcagctt ttccttacag agggtgaata
     2881 aaaaagccgg tacccacaaa tgtggatacc ggcaggtaca aaacagcatg gtcagataaa
     2941 gtggtttttc ccgggcataa atgcttcccc tctctcatcc gccagagaag tatcagtccg
     3001 ttgtgtgcaa ttccgttctg catgatgaag agattcagct gccttttcgc agaccattaa
     3061 ccaccataaa cgatcgatag taacgatcga tacctgtttt atcgatcgtt ttattctatt
     3121 aacgacaagg cggtcacgca agagaaacag gaggagtatg agggggttgt gtcatgtttt
     3181 cagttggtta acgtaatgtt atcaggcatt tccctggcag actgttcaga agaaaatcca
     3241 gttccataca gcgcgggcaa cagagaccac cgtgtcacgg acggcacgca gaaccgtacg
     3301 ggcaacagag gcaacacaga cgctctccgc cgtgtcaaat atccggtcca ccgcaccggt
     3361 aaactgttca cgggcctgag cgcggacaga ttccgtgcgc agctcgtcct gcaggcgggt
     3421 catcagggga ctggcggcat ggtcgggaag tgctgtcatt agcgcactga ccagcgtatc
     3481 cagttcccag ccggtgcggg ccgatacggc aacaaccgga tgtacgggcc ggaacagacg
     3541 gaataccgcc tccgttttct cccgaatgtt ctgtgcctgt gcgggagaag gctgaatgcc
     3601 ggccatatcc cattcatggc agggctccgt tttgtcggcc tgcgtcacca caaacagcac
     3661 ctgctgatgt ccccggtgca ggatgtgtcg ccagaaatac tcatccacag acagggcacg
     3721 gtcatcggct ttaatcagcc acagtaccag gtccagttca ggcagaatgt cacggtacag
     3781 ggcttcatac tctgcatccc tgtcccggct ctcgcccacc ccgggcaggt cagtgataac
     3841 catgctgtga ccatggccac tcagacggaa gcgctgcact tcccgggtgc cggcgtgaac
     3901 atcactgacc ggtgtgacct ccccctgaaa cagggcatta cagagtgagg atttaccggc
     3961 cccgctttta cccataatgc caatcacggg ttcgtgactg gtgagtttac gcagatgttc
     4021 caggatgtga cgtgaaagcg agtaaggcag ggaggaaagc ggtttttcaa ttgcctcaat
     4081 ggcatcagac ggattcatac atgtattccc ggtgaaaaaa gacaaaaccc cgcgcaccgg
     4141 aaaggtggcg ggggatccac ggagataata tctgtctgaa attgttttga atcgatggga
     4201 ttgttgcgta ttaacatgaa ggagatcagg acttatccgt tactgggtga tctccttcca
     4261 tgaaaatacg cggttattgt taaacagcag gctgattact ccgtaaagga gaaaaatatt
     4321 catcttgcag cctgattatc aggtgtgttt atgtttactg aggtgagtat ttttcagtat
     4381 attgtcgggg cagtcagtgg gagatattaa atttacgttt agagttatct tgcttctgct
     4441 gtgtattttt ataagagtgc gcacgtaatg atatctgttg ataccatgtt gttaagtgtt
     4501 tcccttccgg gtcatgcgta cttgcaaaat ggcatctgaa tcgatcacgg tttggccaaa
     4561 ccgtgatcga atagagagga atacgcatgt aatgattaaa tgcttttgtt acatcattct
     4621 tccggcatga atatttatcc agttcccttt tttatttgtg tagtgatact gtcggaatag
     4681 cgttcagtaa tgtaaagtgt cctgttaaaa atctttaata aatgtgtcac cctggtcagg
     4741 aaatatctgc tgagatatta ttagttggtg gtgtttttac tgagtgatag ctggcttttt
     4801 attcctgaac tgaaatactg tcgctcgtca gggtttaatt aaaactggat gatgatgtga
     4861 aaatgaaggc tggtttttgt gtttgtcgag gaagtgttgt ttttatccat tctcatcatt
     4921 ggctggctgc gattatacag aagtatttga agaaaaaatg gtgcatttta tctgtgccgg
     4981 cagggagggg gattttcctg atatgtcagt attaaatggt ttttgttatt tataaaaatg
     5041 tcatcctgtc catagaatat tgtactgagt actcagcatt cagcgcagga actgtcatct
     5101 tctttatttt attctccttc aggagggcag ttttctgaaa gcgttactcc ggggaaactg
     5161 tttatgttta ttcccggcag gaaacccgta cctgcaatac cctggatgat gctttttgct
     5221 ggtacgttgt tgtgtgaaaa ttaatcaaca tgtgtgctcc ggagagcgcc cgtagacgca
     5281 cagattcatt ctgacattct ttagcgacaa aataccgagt ggttattatt taacctgaaa
     5341 atcgacagaa atttttatcc tgtattgatt ctgctgctat ttttgctatt agtagcaata
     5401 gttgcggtac tttatgttct gtgtgttgtg aggtcattat gccatacttt cagtatcctg
     5461 atgagtttcc cctgagctcg ctgcctccac tcattcgtga tgccgttatt gaagctcagc
     5521 aaatcacgca ggctcccctg gggcttgtgg cagcatctgc cctgggcgct gtttcacttg
     5581 tctgccagaa tctgattgat gtctgcagac tgaatacact acgggggccg gtttctcttt
     5641 tcttactgac gcttgctgag tccggagaac gaaaaaccgc agtggacaaa ttattaatgg
     5701 agcccctgta ccagcaggag atgctgttat attccagaca caaaaatgaa ctgacgtcat
     5761 ggaaaaataa ggaagagatt ttaaaggcgc agaaaaaagc attgttgtca aaactgaata
     5821 aagaactccg caaaggcgcg gatgagtcag aaacgctcag acaactggaa gcgcttcaga
     5881 aaaacagaga ggaaaaacct gtaagatata aattcatttt taatgatgcc acgacagcag
     5941 caattaaaga tcagctttgt ggtcagtggc gttcagtggg tatcatgtct gatgaagccg
     6001 gtataatatt tgatgggtat accctgtctg agctgccttt tattaacaaa atgtgggatg
     6061 gctccgtatt gtcggtggac agaaagaatg aacccgaaca gatgattgaa aatgccagaa
     6121 tgacattatc cctcatggta caacccggtc tttttgaccg atatatggag cgaaaaggtt
     6181 ctgttgccag ggacagtggt tttcttgccc gttgtctaat cagtaaacct gccaccacgc
     6241 aggggaaacg ctttattaac ggagctgtga ccccgggagg atcattaacg gcatttcatg
     6301 aacgattaat ggaactggcc agaggcagta ttgaaaaatc cagtaaggat gaacgttatt
     6361 gtctgcattt ttctcctgaa gcgcaaaaaa tatttattga tcactataat gttctggagc
     6421 aggatttgtc tccttccgga cccttaagcc agtttcgggg gcatgtttca aaaaaaacag
     6481 aaaacattgc gagaattgct gcgttattgc agtatttcag tcagggtgaa gggaaaatct
     6541 ctgccgatat catgacgtct gctgtcgtaa taagttcgtg gtacacagat gaatataaaa
     6601 aattatttgc gttacctgat gaatctgaac tgcagctgca ggacgcccgg gagctgctcg
     6661 actggctcat tgaggagtgc cgcggagaat gcccgccccg tgtcaggaaa aattatattc
     6721 tccagtgtgg cccgggccgg ttcaggaaca ggaaaaagct gaacgtcctg ctgaacattc
     6781 ttgcatctca actcaggctc tctgtcgtgc aggaaggaaa aaccacctat gtcctgcttt
     6841 ccgaaataaa caatatcacg ctggataaac tgaatgcgtg ctacagtcag aaactgataa
     6901 gatggcatga gcaatccttt ggcttataat gttccctgtt gctgagacgg attattcagt
     6961 ggtggtatcg cagtatgccc gtcacccccc gcttctgcat gagggtgacg gaggaaaagt
     7021 aagtgtgcgg aggcccggga gcgttatgaa tcaacaggcg tataacaggt actcgtcaat
     7081 catcagttcc agctcgcgct tttcccgttt gcgcgccaca gcccagtttt caagctctgc
     7141 atgtgattta tgctttaact cttcagtcca gggctgcatg gagtgggcac acatcagatc
     7201 ataccagcac agccatatca gcgtgtggta gagatcacga tcgtcgaaac gccggagcag
     7261 gctgttcagc ctgtttgcca gccggttttc acccagacga tgtagctcac tgagtaaaac
     7321 gccgcccaga ttaaaatgtg taagcagcat gatttcatgg gtctgaatgc tggtcattaa
     7381 cccttcctga atggcaggaa tgaacggtgg tgttcccggg ttgacgacct gatagagtcc
     7441 ctggggggcc accgaaatga tggagccttt ttccggcaga aaaaagtgtg ccagggtcag
     7501 acggtgataa ccatgaagat gcggagaccc cattccatga gcgacgataa gcctcgtcac
     7561 gcttgtcatc catttctgat gctggatgag cagcagctca caccggtttt caagtttttg
     7621 cataatattc tctcctttag cgcgcagcaa aaggcctccg ggggccggat catgcctttg
     7681 ctgcggttga acggataacg gtcaggaatt acggacggga ctgtgcaata cgcgcctgca
     7741 gccaggtttc cacttcactt ttcagccagc gggaactgcg gcccagtttg atgggggcag
     7801 gaaaggcacc atccttgatg agcttgtaaa accatttatc ggtcaggccg gtcagttgag
     7861 tgataaatgc catgtcgacc atctggtcat ccatcagtga aactggggta ttcatgaaaa
     7921 tgttcctccg gatgttgtta atctcaaccg gcgttggtga tgaaaacaac gcaccacctt
     7981 caccggcata acaaccgggg agaaacgtaa aaaataatcg ggaatgtgac cgtcaggaga
     8041 taaacggggg gattgcggtg gctgagccgt accggtatgt cgggtgattg ttgcgaaacg
     8101 atgcttccgc acgcgcacgg gaggactttc gtgagacaag gggaaaactg gcgttctgat
     8161 aattgcagag ttgtttttga aggcggttgt ggtgcatcag cctgtgacct gtgggcaggc
     8221 gacaggtatc actggtggga taagactgtc tgattggtat tataaatata taagagagct
     8281 caaactgtag tggtcaagta atactggcca cggttttaca gtaaaaatg
//
LOCUS       DAZKFZ010000198         8320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000198 DAZKFZ010000000
VERSION     DAZKFZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            261..667
                     /gene="ipbA"
                     /locus_tag="ACHOQW_003907"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             261..667
                     /gene="ipbA"
                     /locus_tag="ACHOQW_003907"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
     gene            1093..1887
                     /gene="hyxR"
                     /locus_tag="ACHOQW_003908"
                     /pseudo
     CDS             1093..1887
                     /gene="hyxR"
                     /locus_tag="ACHOQW_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            2041..2802
                     /gene="hyxA"
                     /locus_tag="ACHOQW_003909"
     CDS             2041..2802
                     /gene="hyxA"
                     /locus_tag="ACHOQW_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFP9574900.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            2844..3926
                     /locus_tag="ACHOQW_003910"
                     /pseudo
     CDS             2844..3926
                     /locus_tag="ACHOQW_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            3916..4104
                     /locus_tag="ACHOQW_003911"
     CDS             3916..4104
                     /locus_tag="ACHOQW_003911"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9574901.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
     gene            4286..4954
                     /gene="ykgH"
                     /locus_tag="ACHOQW_003912"
                     /pseudo
     CDS             4286..4954
                     /gene="ykgH"
                     /locus_tag="ACHOQW_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(5197..5892)
                     /gene="ykgG"
                     /locus_tag="ACHOQW_003913"
     CDS             complement(5197..5892)
                     /gene="ykgG"
                     /locus_tag="ACHOQW_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9574902.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5885..7312)
                     /gene="ykgF"
                     /locus_tag="ACHOQW_003914"
     CDS             complement(5885..7312)
                     /gene="ykgF"
                     /locus_tag="ACHOQW_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP9574903.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7323..8042)
                     /gene="ykgE"
                     /locus_tag="ACHOQW_003915"
     CDS             complement(7323..8042)
                     /gene="ykgE"
                     /locus_tag="ACHOQW_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9574904.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2306 a         1938 c         1833 g         2243 t
ORIGIN      
        1 catcaatgta gataaaaaca cgcattaaaa atggcttgta gaaaagcagc acaataaata
       61 gttcatattt agtctattgc gtagaactac tattcaaaaa atcttattaa tgagataatg
      121 agattacatc gataacgttc tgattgcagg catacttatc tgggtgtgcc tctctttttt
      181 acaactgtac acggtggcgt atgacgagaa aatatctcac acaggatgaa gtctacaggc
      241 tgatggatgc agcccagagc atgtcctttc ctgaaagaaa tcgctgtcta attatgatgg
      301 ctttcattca cggttttagg gccagtgaac ttcttgattt acgtttatcc gatattgatg
      361 catcaggaaa acaacttaat attcggcgga ttaaaaatgg tttctcgaca acacatcctc
      421 ttcttcctga tgaatataat ttgatcaagt tgtggcttaa gcagaggaag ttaattgaaa
      481 acggagttga aggagactgg ctttttctct cccggaaacg ccgccctatc agcagacaac
      541 atttttttct atcattcgtg aggctggaag acgtgcagga ttagctgtaa aagcacatcc
      601 tcatatgtta cgtcatgcct gtggttttgc tctggctgac aatggtgtcg atacccgact
      661 acttcaggat tatctgggtc atcgaaatat ccagcatact gtcagataca cagccagtaa
      721 tgctgctcga tttaaagggg tgtggaaaaa aaagcctcgt taaaacagta ccttctgatt
      781 caaaatgtca gattcctacg ttttcctcaa ttcttccata aaaaaacagc ccattcaatt
      841 caataaaaca cataacatgt tgatatttct cgctaaagta aaataacccc atgatttgaa
      901 atgattaacc gccaaggtca taccagtgat taactttcac tcatgttgtt aaatgcaacc
      961 aaattaaaaa caataaaaat caaacaaatt agatttatat tccaagaaaa ttaaatattc
     1021 aggcaattaa acaaaacctg acattttgaa tcaaaatgtc aggttttgta gctatggcgt
     1081 ggacgagtct gaatgaagaa gcctctggtt attatcagtg cctgtcagtt taccaggctg
     1141 gcactggagt ctctcatccc agcggacagg tatatcgtgc gggtgtacag caacgtcact
     1201 acagaggttg aattcgtttt aaagacctcc tgtggctatc tgttggctga tatcccttag
     1261 cttccttcgg gggagttagt tcttctggct tgtcttgtcc gcttgcggag tgatgccggg
     1321 caatggcagg tctgcctgac cggtgactcc gactggttcg ataatgctcc tgctgcaagt
     1381 ctgtataacg gcttcccacg catcggcacc atgctgacag cgagtcgtta tcagaatcaa
     1441 attatccgct ggttactacg tcctttgccc ggcgatatga gcgatttctg gctcctgact
     1501 gcacgggagt tgagcgtgct taagattctg atgcagggca tgagtttcag tgagattgcc
     1561 cgttatgaac aacgcagttc aaagaccctg catgcgattg ccacacgggc attaatgaaa
     1621 cttggactgg gaactctgag cgatttcagg ctgctctata cgggatgcgg tgacagcaga
     1681 gtgaaacgga atatacggct acatgcaaga tatgtcggac aacattccgc tcatcgactc
     1741 actcagtatg tgcagaacat ggtatccgga attctttatc cagagaaagt tgccgttggt
     1801 gaatcaataa attctgacgc catttccgga aaagtacttc ccggacaaag aaaaaatatt
     1861 tctaaaaaaa gaccaggatt gttatgacac aacagcgttg ttactacccc ccactgcggc
     1921 gcgtacggac tcgctacagt gtaggggcca aaatatttct actgctctgg gctgcaggat
     1981 tgggaggcac gctgtacagc ctgtacagcg tgctggcgta ctgacatctg tgagggaagg
     2041 atgcgtttcg ttcttttctg tccctcagga attgtaccag cgcagttcgc agctctatca
     2101 accggttctg tcggcaatgt tacctgcatc ggaacagaag aggagttacg caataaattg
     2161 cggcacaggc cacagagtgt cgtgatatcg gcagggcgac ctgccgaatg cgctgaaatg
     2221 tggttccgat tttatcggga tcactcattc gtggtggtgt tgtgcgtcgc gccatttttt
     2281 ctgccaccgg atgtgagtat ttctggcgtt ctcaaaaacc tgaggttact gaaaccagga
     2341 atgtcagtag agcatgtcat cagtattgca aattcctctg gtgggttatc agggctgaaa
     2401 catgcagaaa atttgccagt tatggacagt tactctgtct ttatgaaaga ggtgaataac
     2461 cgcacaaaga caatcgtcat gtcagaacgc ttcccggaaa agcaaaagaa ggtgttgtcg
     2521 ttgcttcttg ccggacacag ctgggaatac agtgcgcagt ttctcaaaac ggggataaga
     2581 cagatatggc ttgccgagca gtcgctaaaa aagcgttggg gaataccgga cagcatgtca
     2641 ttgcgggaag ccctgctgct gagcagcaat aaatttcacg gtgatggaaa tgcattggaa
     2701 aaaacgaatg ccatgacatt gcgtgaaaac ggaaatacca acaggtattc cgtggtggta
     2761 aatgcaggga cgcaggtttt atctctgcat aaatataaat aagtgggaaa ttgaaattag
     2821 ttaccagaat ggagaatttc ttcatgaaaa acagtaaggc attttaccgc agcgcattag
     2881 cgacagctat tgttatggct ctttctgcac cagcattcgc tgctgataac gcggtatcaa
     2941 ctgattcggt tacgctgaat aaagataaga cgactctgga tcaagatgtt gttattagca
     3001 atacagcaga caaacagatt acagccgtaa caattaatgc ggcagatgaa gaccttaatg
     3061 ttgcttttgc tggtcatgat attactgctg aatcaacagc agacaaaaaa ttcgttgaag
     3121 gtgtaaaagt tagtggtaac aaaaatgttg tgattaatgc tacagactcc accatcacag
     3181 ctcaaggtga aggcacctat gtccggactg caatggtcat tgattcaact ggcgatgttg
     3241 ttgttaatgg cggtaatttc gttgcaaaaa atgaaaaagg tagtgcgaca gggatatctc
     3301 tggaaggggc caagggaaat aatgtcacgc ttaatggcac aaccataaat gctcaaggta
     3361 ataagagttc cagcaacgcc tccacggcaa tttttgctca aaagggtagc ttattgaata
     3421 gttttgaagg tgatgcaacc gacaacatta cccttgctgg ctcaaatatt attaatggtc
     3481 ggattgaaac aatagttact gccgggaata agacgggaat tcatacagtc aacctgaata
     3541 ttaaggatgg ctcagtaatt ggggcggcta ataataaaca aacaatttat gcctctgctt
     3601 cggcacaagg cacaggttca gcaacgcaaa atttaaattt gtctgttgct gattcaacca
     3661 tctactctga tgtcctggcc ctttctgaaa gcgagaattc agccgctacc acaacaaatg
     3721 taaacatgaa cgttgcccgc tcttactggg aaggtaatgc ttatactttc aatagcggcg
     3781 ataaagcggg tagtaatctg gatataaatc tttccgatag ttcagtctgg aaaggcaaag
     3841 tttcaggggc aggaaatgcc agtgtatctc tgcaaaacga gtctgtctgg aatgttacgg
     3901 gatcctcaac tgttgatgct ctggctccgt ctgggtgatg agtctgtcaa tgccagcatt
     3961 gacggtgcag cattccgcgt gggtgcaggt gtacaggctg atatcaccaa aaacatggga
     4021 gcatatgcaa gccttgacta caccaaaggt gacgacattg agaatccgct acagggtgta
     4081 gttggtatca atgtgacctg gtaagaataa agcagtcggt tcccctgtcg actgttatac
     4141 cccctgcagg cagcagtatg ctgcctgcag gctataaata tccagtattc atgatgctgg
     4201 ctttttgctg atatttaacc cgaacattcc tgatatagac tattaattca tttatctgcg
     4261 gcagatttta ttttctggat atattatgag tgaacaaatc aaacaggata tcgatctgat
     4321 tgagatttta ttttatctga agaaaaagat tcttgttatc ctttttatta tagctatctg
     4381 tatggcaatg gtgcttttat ttctgtatat caataaagat aatataaaag tgagttacag
     4441 cctaaaaata aatcagacaa cacccggcat acttgttaac tgtgatagca ataataactt
     4501 tgcctgtcag acaacaatga cggaagatgt tattcagcga attactacat tttttcacac
     4561 cagcccagat gtcaagaaca gagaaataaa gctggaatgg tcaggagata agagagattt
     4621 accaactgct gaagcggaaa tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc
     4681 agaataccat aatggcaggt aagttctcga tgagatacaa acgccttcag caattaacag
     4741 tgagctttat acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga
     4801 tggctgtgta actataagct caccagaaat aaaaaataaa taccctgctg ccatttgcct
     4861 ggctctggga ttttttctaa gcattgtaat ttctgtaatg ttttgtcttg tcaaaaaaat
     4921 ggtagatgaa taccaacaaa actctgggca gtaataaaca gcattctgat agagagtgcc
     4981 tgtataaagc tgaagtgaca atgaccggga agggaacccg ttttcaggaa tggatgcctg
     5041 tctttttcac tgtaaatatc ctggcattag taatgccagg atatttacag aagaggagca
     5101 taaccatgac ggaacggggt taagtcaggt tgtccttaac gctattttca acctcgtatg
     5161 ccttcttcag gtttatgtcc agacttcata cctctctcag caatcctcaa taatcagata
     5221 caccgctttc accggaccat gaaccccgac gactttgata agctcaatgt ccgccgttga
     5281 gctgggacca ctgatgatgt taatacatga cggcatgcgt tcaccggcct gcgctttctg
     5341 atgcaatctt tctgcgagtt gcgctacacg cggcaggata gtgcttttac gcaggataaa
     5401 aagagatgat tccgggagca ggctcaatga acgcccacgc tcggcggcgg aaaaaagaac
     5461 cacgcctccc gattcggtta aaccatattc agcatacacg acacccactt tagcctgctc
     5521 tgcctgcgag atattcttgg cacctttcgc cggatcccaa acaacggcat tgcattcctg
     5581 ctgcaaacgt tcgctaatcc ctaattcctc cagcctcgtg tcaccgctaa tcacgaccga
     5641 ctgatctccc agctctttac acagacgtat tgcagcttct gccgccttcg cctcgctggt
     5701 cagctcacag cgcgtcaaca taacatcgct ggcaaactga ataaacgcgt cacagcgctg
     5761 ctgttggtta agttgggtaa gccgctcgtt agcatagtta ttaagcggcg catcttctgc
     5821 ttgcggttca agtcgcagcg ggcgacccag tgcctgagca acgttattca aaaattcgcc
     5881 tcgattatcc attctttttc tcctgcgcct gatgtttctt aaaccaacta cggaaactct
     5941 ctccgtcagc ttcaggaaga tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga
     6001 gtggtgtttt gccgccattg ataaaccagc ttgccgcatg agcaccggcc atcatcccga
     6061 ctttccacaa tcctggatga ctattggcat aagcgaacat ttttatcgcc cgttgctctg
     6121 cttttgcggt gatccctttt tcagccatca cccgacgatg acgcaaaatc agttttgaca
     6181 gcggaatacg caccggacac acgttgtcac aagctgtgca taaagagcag gcgtagggta
     6241 aatctttaaa atctttatag ccgccaagta gcggagaaat caccgcacca attggccctg
     6301 gataaataga gccatatcca tgaccgccaa tatggcgata tgccggacaa gtattcatac
     6361 aagccccgca gcgaatacag cgcagcacat cccgaaattc agaggccagc acctcagaac
     6421 gcccgttatc gacaataacc agatgaaact cttcaggacc atcaacgtgc cccgcttcgc
     6481 gaggtcctgt cagccaggtg ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca
     6541 gcatggtgat caatacatct acctcggcaa acgtgggggc aatacgctcc attcccatca
     6601 ctgcaatatg cgttttaggc agcgtggtac acattcgcgc attaccttca ttggtcacca
     6661 ggcataccga acctgtctct gccaccgcga aattacagcc cgtaatacct atttcagcac
     6721 tgaggaaatc ttcgcggatt ttttgccgga tgaataaggt catcgcttca ggcgtttccg
     6781 gcccctcata gcccagacgt tcgtgtagca ctcgacggat ctgatggcga tctttatgaa
     6841 ttgccgggac cacaacatga gagggcggat cttgatccag ctggagaata tattcaccca
     6901 aatcggtttc aatcacctga atgccagcat cctgcaacac atgattgaca ccaatctctt
     6961 cggtcaccat cgatttagat ttcaccacct tccgggcatt tttgcgttgg gcaacctgta
     7021 aaatgtagcg ggtagcgtct tctttggttt ttgcaaaata gacgtgaccg ccgttttgcg
     7081 tcactttttc tgagagctgg tacagataag cgtcgagatt actcagaaca tgatcacgta
     7141 tctgggcggc ccgatcgcgc cactcctccc agagccccaa ttcatcgacc attttttgcc
     7201 gatttgcccc aatacgctgc tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa
     7261 tttgctgacg gatgcgtgtc ttaaaatctg tattactggt tttgatcgac atctttatat
     7321 cctcagcggc tcatcaacac ttcagcaata tgcatcactt tgactttctg cccttcccgt
     7381 tgtaatcgcc cactgatgtt cagcaggcaa ctcacgtcag caccaattaa atactcaggg
     7441 cggacttcca tcaggtgcgc aaccttttct ttcaccatct cgccggatat ttcggccatt
     7501 ttgaccgaga acgtgccgcc aaatccgcag caggtatcct gttcagcaaa ggtcaacagc
     7561 tccagtccac gcacattttt cagcagcgta agtggctcgt ccttcacccc cagcttacgg
     7621 gccaggctac aagatgggtg atacaccgct ctcccttgca aactggcacc tacatcgact
     7681 acccctaatt tattaacaat aaaagaggtg agatcctgca tacgcgcggc aaccttttcg
     7741 gctcgtgatg cccattcagg ttcatccgcc agatacgtcg ggtaactttt tacggcatag
     7801 gtgcaagagc cagccggtga aataatggga tcgtcgttat cctccagtgc ggcgatcaga
     7861 tttttcatcc ctggaatcgc ttctttgata taaccactat tgatcgcagg ctgaccgcag
     7921 catccctgtt tctccgggaa atttacgcga cagccgagtt tttccagtag cagcacggag
     7981 tctcgtgcca ttcttgattt cagggcgtca ccaatacagg tgacaaagaa attgacattc
     8041 acaactaata ctccattact tcatgcccat ttattcccga tgtttccccc cacccgggga
     8101 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc accccttatt
     8161 tgtatgacaa atgatagtct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     8221 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     8281 taaaaatggc tacatccctt atacctattc agagctactt
//
LOCUS       DAZKFZ010000199         8279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000199 DAZKFZ010000000
VERSION     DAZKFZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            9..746
                     /gene="ygaZ"
                     /locus_tag="ACHOQW_003916"
     CDS             9..746
                     /gene="ygaZ"
                     /locus_tag="ACHOQW_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP9574905.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            736..1071
                     /gene="ygaH"
                     /locus_tag="ACHOQW_003917"
     CDS             736..1071
                     /gene="ygaH"
                     /locus_tag="ACHOQW_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP9574906.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            1162..1692
                     /gene="emrR"
                     /locus_tag="ACHOQW_003918"
     CDS             1162..1692
                     /gene="emrR"
                     /locus_tag="ACHOQW_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP9574907.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            1819..2991
                     /gene="emrA"
                     /locus_tag="ACHOQW_003919"
     CDS             1819..2991
                     /gene="emrA"
                     /locus_tag="ACHOQW_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP9574908.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            3008..4546
                     /gene="emrB"
                     /locus_tag="ACHOQW_003920"
     CDS             3008..4546
                     /gene="emrB"
                     /locus_tag="ACHOQW_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP9574909.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(4610..5125)
                     /gene="luxS"
                     /locus_tag="ACHOQW_003921"
     CDS             complement(4610..5125)
                     /gene="luxS"
                     /locus_tag="ACHOQW_003921"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9574910.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(5275..6831)
                     /gene="gshA"
                     /locus_tag="ACHOQW_003922"
     CDS             complement(5275..6831)
                     /gene="gshA"
                     /locus_tag="ACHOQW_003922"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP9574911.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(6904..7332)
                     /gene="yqaA"
                     /locus_tag="ACHOQW_003923"
     CDS             complement(6904..7332)
                     /gene="yqaA"
                     /locus_tag="ACHOQW_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP9574912.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(7329..7895)
                     /gene="yqaB"
                     /locus_tag="ACHOQW_003924"
     CDS             complement(7329..7895)
                     /gene="yqaB"
                     /locus_tag="ACHOQW_003924"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP9574913.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(8176..8252)
                     /locus_tag="ACHOQW_003925"
     tRNA            complement(8176..8252)
                     /locus_tag="ACHOQW_003925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8216..8218),aa:Arg,seq:acg)
BASE COUNT         1939 a         2201 c         2089 g         2050 t
ORIGIN      
        1 tacactctat ggaaagccct actccacagc ctgctcctgg ttcggcgacc ttcatggaag
       61 gatgcaaaga cagtttaccg attgttatta gttatatccc ggtggccttt gcgttcggtc
      121 tgaatgcgac ccgtctggga ttctctcctc tcgaaagcgt ttttttctcc tgcatcattt
      181 atgcaggcgc gagccagttc gtcattaccg cgatgctggc agcagggagt agtttgtggg
      241 ttgctgcact gaccgtcatg gcaatggatg ttcgccatgt tttgtatggc ccgtcactgc
      301 gtagccgtat tattcagcgt ctgcaaaaat cgaaaaccgc cctatgggcg tttggcctga
      361 cggatgaggt ttttgccgcc gccaccgcaa aactggtacg caataatcgc cgctggagcg
      421 agaactggat gatcggcatt gccttcagtt catggtcatc gtgggtattt ggtacggtaa
      481 taggggcatt ctccggcagc ggcttgctgc aaggttatcc cgccgttgaa gctgcattag
      541 gttttatgct tccggcactc tttatgagtt tcctgctcgc ctcttttcag cgcaaacaat
      601 ctctttgcgt taccgcagcg ttagttggtg cccttgcagg cgtaacgcta ttttctattc
      661 ccgtcgccat tctggcaggc attgtctgtg gctgcctcac tgcgttaatc caggcattct
      721 ggcaaggagc gcccgatgag ctatgaggtt ctgctgcttg ggttactggt tggcgcggcg
      781 aattattgct tccgctattt gccgctgcgc ctgcgtgtgg gtaatgcccg cccaaccaaa
      841 cgtggcgcgg taggtatttt gctcgacacc attggcatcg cctcgatatg cgctctgctg
      901 gttgtctcta ccgcaccaga agtgatgcac gatacacgcc gtttcgtgcc cacgctggtc
      961 ggcttcgcgg tactgggtgc cagtttctat aaaacacgca gcattatcat cccaacactg
     1021 cttagtgcgc tggcctatgg gctcgcctgg aaagtgatgg cgattatata acccacaaga
     1081 atcatttttc taaaacaata catttacttt atttgtcact gtcgttacta tatcggctga
     1141 aattaatgag gtcataccca aatggatagt tcgtttacgc ccattgaaca aatgctaaaa
     1201 tttcgcgcca gccgccacga agattttcct tatcaggaga tccttctgac tcgtctttgc
     1261 atgcatatgc aaagcaagct gctggagaac cgcaataaaa tgctgaaggc tcaggggatt
     1321 aacgagacgt tgtttatggc gttgattacg ctggagtctc aggaaaacca cagtattcag
     1381 ccttctgaat taagttgtgc tcttggatca tcccgtacca acgcgacgcg tattgccgat
     1441 gaactggaaa aacgcggttg gatcgaacgt cgtgaaagcg ataacgatcg ccgctgcctg
     1501 catctgcaat taacggaaaa aggtcacgag tttttgcgcg aggttttacc accgcagcat
     1561 aactgcctgc atcaactctg gtccgcgctc agcacaacag aaaaagacca gctcgagcaa
     1621 atcacccgca aattgctctc ccgtctcgac cagatggaac aagacggtgt ggttctcgaa
     1681 gcgatgagct aacgcgtcat ctcgctcaaa aatccagatt tataaaagaa aaaatgaccg
     1741 gccagcatcg caacatgctg gcctttttgg caagcaggtc ggctcagccg atgagttaag
     1801 aagatcgtgg agaacaatat gagcgcaaat gcggagactc aaaccccgca gcaaccggta
     1861 aagaagagcg gcaaacgtaa gcgtctgctc ctccttctca ccttgctctt tataattatt
     1921 gccgtagcga tagggattta ttggtttttg gtactgcgtc acttcgaaga aaccgatgac
     1981 gcatacgtgg cagggaatca aattcaaatt atgtctcagg tgtctggcag cgtgacgaaa
     2041 gtctgggccg ataacaccga ttttgtaaaa gaaggcgacg tgctggtcac tctcgacccg
     2101 acagatgctc gccaggcgtt tgaaaaagcc aaaactgcac tggcttccag cgttcgccaa
     2161 acccaccagc agatgattaa cagcaagcag ttgcaggcga atattgaggt gcaaaaaatc
     2221 gccctcgcga aagcacaaag cgactacaac cgccgtgtgc cgctgggcaa tgccaacctg
     2281 attggtcgcg aagagctgca acacgcccgc gacgccgtta ccagtgccca ggcgcaactg
     2341 gacgtcgcga ttcaacaata caatgccaat caggcgatga ttctggggac taaactggaa
     2401 gatcagcctg ccgtgcaaca ggctgccacc gaagtacgta acgcctggct ggcgctggag
     2461 cgtactcgta ttgtcagtcc gatgaccggt tatgtctccc gccgcgcggt acagcctggg
     2521 gcgcaaatta gcccaacgac gccgctgatg gcggtcgttc cagccaccaa tatgtgggtg
     2581 gatgccaact ttaaagagac gcagattgcc aatatgcgta tcggtcagcc ggtcactatc
     2641 accaccgata tttacggcga tgatgtgaaa tacaccggta aagtggttgg tctggatatg
     2701 ggcacaggta gcgcgttctc actgcttccg gcgcaaaatg cgaccggtaa ctggatcaaa
     2761 gtcgttcagc gtctgcctgt gcgtatcgaa ctggaccaga aacagctgga gcaatacccg
     2821 ctgcgtatcg gtttatccac gctggtgagc gtcaatacca ctaaccgtga cggtcaggta
     2881 ctggcaaata aagtacgttc cactccggta gcggtaagca ccgcgcgtga aatcagcctg
     2941 gcacctgtca ataaactgat cgacgatatc gtaaaagcta acgctggcta atccagaggt
     3001 gcgtgtgatg caacagcaaa aaccgctgga aggcgcgcaa ctggtcatta tgacgattgc
     3061 gctgtcacta gcgacattca tgcaggtgct ggactccacc attgctaacg tggcgatccc
     3121 cactatcgcc gggaatcttg gctcatcgct cagccaggga acgtgggtaa tcacttcttt
     3181 cggggtggcg aatgccatct cgatcccgct taccggctgg ctggcaaagc gcgtcgggga
     3241 agtgaaactg ttcctctggt caaccatcgc ctttgctatt gcgtcgtggg cgtgtggtgt
     3301 ctccagcagc ctgaatatgc tgatcttctt ccgcgtgatt caggggattg tcgccgggcc
     3361 gttgatcccg ctttcgcaaa gtctattgct gaataattac ccgccagcca aacgctcaat
     3421 cgcgctggcg ttgtggtcga tgacggtgat tgtcgcgcca atttgcggcc caatcctcgg
     3481 cggatatatc agcgataatt accactgggg ctggatattc ttcatcaacg tgccgattgg
     3541 cgtggcggtg gtgttgatga cactgcaaac tctgcgcgga cgtgaaaccc gcaccgaacg
     3601 gcggcggatt gatgccgtgg ggctggcact gctggttatt ggtatcggca gcctgcagat
     3661 tatgctcgac cgcggtaaag agctggactg gttttcatca caggaaatta tcatccttac
     3721 cgtggtggcg gtggtggcta tctgcttcct gatcgtctgg gagctgaccg acgataaccc
     3781 gatagtcgat ctgtcgttgt ttaagtcgcg caacttcacc atcggctgtt tgtgtatcag
     3841 cctcgcgtat atgctctact tcggcgcgat tgttctgctg ccgcagttgt tgcaggaggt
     3901 ctacggttac acggcgacct gggcaggttt ggcctctgcg ccggtaggga ttattccagt
     3961 gatcctgtcg ccgattatcg gccgcttcgc gcataaactg gatatgcgcc gactggtaac
     4021 cttcagcttt attatgtatg ccgtctgctt ctactggcgt gcctatacct ttgaaccagg
     4081 tatggatttt ggcgcgtcgg cctggccgca gtttatccag gggtttgcgg tggcctgctt
     4141 ctttatgccg ctgaccacca ttacgctgtc tggtttgcca ccggaacgac tggcggcggc
     4201 atcgagcctc tccaacttta cgcgtacgct ggcggggtct atcggcacgt cgataaccac
     4261 aaccatgtgg accaaccgcg agtcgatgca ccatgcgcag ttgactgagt cggtaaaccc
     4321 gttcaacccg aatgcccagg cgatgtacag tcaactggaa gggcttggga tgacacaaca
     4381 gcaggcatca ggctggattg cccagcagat caccaatcag gggctgatta tttccgccaa
     4441 tgagatcttc tggatgtcgg ccgggatatt cctcgtcttg ctggggctgg tgtggtttgc
     4501 taaaccgccc tttggcgcag gtggcggcgg aggcggtgcg cactaagtac aactaagcca
     4561 gttcatttga actggctttt ttcaattaat tgtgaagata gtttactgac taaatgtgca
     4621 gttcctgcaa cttctctttc ggcagcgcca gttcttcgtt gctgttgatg cgcacgtcac
     4681 gttccagaat gctacgcgca atatcctgcg cttcctgcaa cgagtgcatc tggtaagtgc
     4741 cacactggta gacgttcagc tccggaatct gattctgatc ctgcactttc agcacgtctt
     4801 ccattgccgc tttccagaca tcagcaacac gctgctcatc tggcgtacca atcagactca
     4861 tataaaaacc ggtgcggcag cccattggcg agatatcgat aatctctaca ccattaccgt
     4921 taagatggtt acgcataaaa ccagcaaaca ggtgctccag ggtatggatc cctctttctg
     4981 gcatcacttc tttgttcggc acgcagaagc gcagatcgaa cacggtgatt gcgtcgccat
     5041 gcggggtgtt cattgttttc gccacccgaa ctgcaggcgc ttccatccgg gtatgatcga
     5101 ctgtgaagct atctaacaac ggcatttagc cacctccggt aattttttta aaaattttct
     5161 gaactctttc ttcccaggcg agtctgagta tatgaaagac gcgcatttgt tatcatcatc
     5221 cctgaattca gagatgaaat tttggccact catgagtggc ctttttcttt tctgtcaggc
     5281 gtgtttttcc agccacaccg caaacggttc ggtatctgcg gcttccattt cctgctgacg
     5341 gcgttcagat gcctcgcgct cggctacaaa atcctcttcg cgcagaattt ccagcggctc
     5401 ttcacgcagc agattacggt acgcttcagc aaacgctttg cctgttccgc caataccagt
     5461 atcaatcata gaccttaaga tacgggcaga gaaagtcaga tcgggattat cgaagcaggc
     5521 aaccagttca tcacacactt tctgatacgc ttcgccgccg ttaatgctat ccagcgtttg
     5581 cgcgacgcgt ttcagatcgc ggaacagatc tttacccacc tgcggtaacg ggaactgtgc
     5641 ggtttcgcag ccgataccca gcgtcagacc cggtttgcga ccttcgagga tcacccggtt
     5701 ccagttaacg cgtgtacagg caagttcgct actgctcatt tccggtgcat cagccagcgc
     5761 acaccagacc ataaacaggt cgaggaatcg cacctgctgt tcatctacac caatcggcga
     5821 gaacgggttg atgtccagcg aacgcacttc aatatattca atgccgccac gtaacagcgc
     5881 atcagaaggc gactcgccgc tgcgggtaac gcgttttgga cgaatcggcg cgtacagttc
     5941 gttttcaatc tgcagcacgt tgctgttgat ttgcagcctc ttaccgtctt tatcaatacc
     6001 aatcttcgcg tactcttccg atggcgtttt gattgcctgt ttcaggcccg ctacgtactc
     6061 gtaaagatcg ttgaaggtaa taccaagatt gctttgcgat ttattggtat agccgagatc
     6121 gctcaaacga agagaggtcg catacggcag gtaatacata ccgcactcgg ttttctcaaa
     6181 cggcagcgac gttggtttac cttgcaggaa tgaagagcat attgccggag acgcgccaaa
     6241 cagataagga atgacccaac cgaagcgata gtagttgcga ataacgcgga aatagcccgc
     6301 agagattttc tctttagcat cagcgcccga aatatcgcca cacttcgctt gccagaatgc
     6361 cataggcaaa gagaagttgt aatgcacgcc ggagatggtt tgcatcagcg cgccgtagcg
     6421 atttttcagc ccttcacggt acagcgtttt aaagcgtccg gtgttagaag tgccgtactg
     6481 cgccagttcg atgtcctgac cttctgcgat gtagcatggc atacttaacg gccacatccg
     6541 ctcatcgccc atattgcgcg ccgtataacg atgcagatcg cgcataaagg tcagcatatg
     6601 ttcaatatca ccatccactg gtgtaatgaa ttccagcaat gcttccgcaa aatcggtagt
     6661 aatccattta tgcgtcagtg cggaacctaa tgcttcagga tgacctgttg ttgccagtgt
     6721 gccatcagca ttaacacgca aagtttcgcg ctccagccca cgctgtatcc cctttaacgc
     6781 ctgaggatgt ttttccagcc aggccagcgc ctgtgatacg tccgggatca aattgacctc
     6841 ccgcctgtca aaatcgtttt aattagcata actgtaatgg tgaccatatg tgcaggccta
     6901 caattagtgc caccacatca tgccctgaac ggtcgctgct gcaactgcaa catagcgtaa
     6961 cgctttacca aggcacaaaa aaaagattac cggcccccag gagatgcgca tccatcccgc
     7021 taacagacac agtaaatcgc caaccacggg catccagctt aataatagcg tgactgcacc
     7081 atagcgtttc agccagccgg tagctttctc ttgccagcgc gatgttttac gcaatggaca
     7141 gaaacgccca aggataacgt tagttaaccc tccaaggcta ttacccattg ttgctgttaa
     7201 aactaaaacc cagggatgac tgatcccaga aagcaacatt gccaccagca cgacttcgga
     7261 gttgccgggt aatagtgtag cgctgagaaa actactggca aacaacgaga aaagcgataa
     7321 cgcttcactc acagcaagcg aacatccaca gcgtccatgc ctgctgcacg ggccgcctga
     7381 ataccgaaat cggcatcttc aaagaccaca cactgcgtcg gttgcacgcc catacgctgc
     7441 gcgcacaaca aaaatgtgtc tggcgcgggt ttatggtgtt tgacgtgatc ggcagcgacg
     7501 acggcgtcaa aataacggcg taatcccagg tgagccagca atgcctcagc gatggcgctt
     7561 tcactccccg ttcctacagc cattgggcga cgaccatgcc aacttttcac cacttcaaca
     7621 agaggaagcg gctcgacact atccagcagc atacttctta ctgcttctgt tttttcacgc
     7681 gctaacgcat gcgggtcgag atcggcctga ttcagctcaa taattgcctg agcaatacgc
     7741 caggtgggcg atccattaag cgcaatcatc gcctgaacat cgtactgaag accgtagtgc
     7801 cctaatactt cgcgccacgc tttacggtgc gtaggctccg tatccaggat tgtgccatcc
     7861 atatcaaaaa ttaaacctgc ataacgctcg tacatggtct tctcgcaagt cgaaaaatat
     7921 gacgctactt tagcgtaatt gcttgatttt gtcgctgatg gcgaaggaga agaaaattca
     7981 ggaaatggat aaagtagtta tttagaaatg agatattttt gaaggaaatt ttttggagaa
     8041 gatggtgcat ccgggaggat gactcactgg cgttcgccct tcgggccgtt gctaaagcaa
     8101 cgttatcctt cctggtgcct gcggttaatt ccgctacctc ttcgatatct tcattgctga
     8161 agatttcgga gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
     8221 ccgagtactc tatccagctg agctacggat gcatcgggaa aatttatttt actgctgat
//
LOCUS       DAZKFZ010000200         8272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000200 DAZKFZ010000000
VERSION     DAZKFZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            298..1086
                     /gene="fabI"
                     /locus_tag="ACHOQW_003926"
     CDS             298..1086
                     /gene="fabI"
                     /locus_tag="ACHOQW_003926"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9574914.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1230..2357
                     /gene="yciW"
                     /locus_tag="ACHOQW_003927"
     CDS             1230..2357
                     /gene="yciW"
                     /locus_tag="ACHOQW_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP9574915.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            2425..4359
                     /gene="rnb"
                     /locus_tag="ACHOQW_003928"
     CDS             2425..4359
                     /gene="rnb"
                     /locus_tag="ACHOQW_003928"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP9574916.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(4478..4540)
                     /locus_tag="ACHOQW_003929"
                     /pseudo
     CDS             complement(4478..4540)
                     /locus_tag="ACHOQW_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4594..6579
                     /gene="pdeR"
                     /locus_tag="ACHOQW_003930"
     CDS             4594..6579
                     /gene="pdeR"
                     /locus_tag="ACHOQW_003930"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9574917.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(6561..6689)
                     /gene="ymiD"
                     /locus_tag="ACHOQW_003931"
     CDS             complement(6561..6689)
                     /gene="ymiD"
                     /locus_tag="ACHOQW_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP9574918.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            6727..6900
                     /gene="yciZ"
                     /locus_tag="ACHOQW_003932"
     CDS             6727..6900
                     /gene="yciZ"
                     /locus_tag="ACHOQW_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP9574919.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            6990..7739
                     /gene="yciT"
                     /locus_tag="ACHOQW_003933"
     CDS             6990..7739
                     /gene="yciT"
                     /locus_tag="ACHOQW_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP9574920.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            8008..8226
                     /gene="osmB"
                     /locus_tag="ACHOQW_003934"
     CDS             8008..8226
                     /gene="osmB"
                     /locus_tag="ACHOQW_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP9574921.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         2072 a         2069 c         2001 g         2130 t
ORIGIN      
        1 aaggctgcta cttgttgtcg ccagttctgt tttcatcatc aaacttatcg gagctaatac
       61 gaatagtttc atagtcagat tctataagat atttccccaa cctacccctt gcaggcgtca
      121 agagtagata acgcaaaatg ctctgtaccg cagcttctct ccggtacaga aagcgcaact
      181 atagccaccc acagccaggt tgattataat aaccgtttat ctgttcgtac tgtttactaa
      241 aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat taaagctatg
      301 ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact gtccatcgcc
      361 tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac ctaccagaac
      421 gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttctga catcgttctg
      481 caatgcgatg ttgccgaaga tgccagcatc gacaccatgt tcgctgaact ggggaaagtt
      541 tggccgaaat ttgacggttt cgtacactct attggttttg cacctggcga tcagctggat
      601 ggtgactatg ttaacgccgt tactcgtgaa ggcttcaaaa ttgcccacga catcagctcc
      661 tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg ttctgccctg
      721 ctgacccttt cctaccttgg cgctgagcgc gctatcccga actacaacgt tatgggtctg
      781 gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg tccggaaggt
      841 gtgcgtgtta acgccatctc tgctggtccg atccgtactc tggcggcctc cggtatcaaa
      901 gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg taccgttact
      961 attgaagatg tgggtaactc tgcggcattc ctgtgctccg atctctctgc cggtatctcc
     1021 ggtgaagtgg tccacgttga cggcggtttc agcattgctg caatgaacga actcgaactg
     1081 aaataatcgt tctgttggta aagatgggcg gcgttctgcc gcccgttatc tctgttatac
     1141 ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg cgtcaggata
     1201 acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc ggcaaaagcc
     1261 actggtatca tgaaacgcaa tccagtacta cggagtatga cgttctgcct ctggtcccgg
     1321 aagccgcaaa ggtcagcgat ccctttctac tcgacgtgat ccttgaaaaa gaaacgctgg
     1381 cccccttcct ttcatggctg gaccctgcgc gtgttcttgc agtggagttg ttccctgacc
     1441 agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtcg acggccctga
     1501 cggttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg cgacttacgc
     1561 cgctccccgg gcctgattcc accagggaaa gtaatcatcg gttggcacaa atcacgcaat
     1621 atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcgc cagcatctga
     1681 atgacgtcgg tcttactgcc tgggactgtg tgatcattaa ccaaatcatt ggttttattg
     1741 gctttcaggc gcggacaatt gcgacatttc aggcttatct cgggcatccg gtacgctggt
     1801 tacccgggct ggagatacaa aactacgccg acgcgtcact gtttgctgat gaatcattac
     1861 gctggcgaag cagctatgag gtggaaaaac tacctgaaga acacacaaaa agttcaactg
     1921 cagaactttg ccaactggcc gaaatactct ctctccaccc tatttcactt tcccttctcg
     1981 aaaggttgtt aaacagcaca cgggttaata cacagccgga taatcagctt gcggcgttgt
     2041 tatgcgcgcg gataaatggc agtcctgctt gttttgccgc ctgtatggat tcatcaaatg
     2101 aatataaaaa aatcagcccc cttctgcgca agggcgaaaa tgaaattaac caatgggctg
     2161 accgtcattc tgttgagcgc gctaccgttc aggcgataca atggctgacc cgagcacccg
     2221 atcgctttag cgccgcccag ttcagccctt tactcgaaca cgaaaaatca tcaacgcaga
     2281 ttattaatct gctggtatgg agcgggctgt gtggctggat aaatcgctta aaaatcgcgt
     2341 tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg tcagccgctc
     2401 taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc gcagcttaaa
     2461 cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac agaaaaaggt
     2521 tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc gcagatgaaa
     2581 aaagtcatgc atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga acgtgaatcc
     2641 gcagagccag aagaactggt tgaaccgttt ctgactcgtt tcgtgggtaa agttcagggc
     2701 aaaaatgacc gtctggccat cgttcctgat catccactct taaaagacgc cattccttgc
     2761 cgcgcagccc gtggtctgaa ccacgagttt aaagaaggcg actgggcagt tgccgaaatg
     2821 cgccgtcatc cgctgaaagg cgatcgttct ttctatgctg aactgacaca atacatcact
     2881 tttggtgacg accactttgt accgtggtgg gttacccttg cgcgccataa tctggaaaaa
     2941 gaagcaccag acggcgtcgc taccgaaatg ctcgatgaag gtctggttcg taaagatctg
     3001 accgcgctgg attttgtcac catcgacagt gccagcacag aagatatgga tgacgccctt
     3061 ttcgctaagg cgttgccgga tgacaaactt cagctgattg tggcgattgc cgatccaacc
     3121 gcgtggattg ctgaaggcag caagctggac aaagccgcga aaattcgcgc attcaccaac
     3181 tatctgcctg gcttcaacat ccctatgctg cctcgcgagc tttctgacga tctctgctca
     3241 ctgcgcgcca atgaagtccg cccggtactg gcatgccgca tgacgctctc cgctgatggc
     3301 accattgaag ataatatcga attctttgcc gccaccatcg aatccaaagc gaagctggtg
     3361 tatgaccagg tttctgactg gctggagaat accggtgact ggcagcctga aagtgaagca
     3421 attgccgaac aagtccgttt gctagcgcaa atttgccaac gccgcggcga gtggcgtcat
     3481 aaccacgcac tggtgtttaa agatcgcccg gattaccgct ttattctcgg tgaaaaaggt
     3541 gaagtgctgg atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt cgaagaagcg
     3601 atgattgccg ctaacatttg tgcagctcgc gtactgcgcg ataagctcgg ttttggtatc
     3661 tataacgtgc atatgggctt tgatccggcg aatgccgacg cgctggcagc gttgctgaaa
     3721 acgcacggtc tgcatgtcga tgccgaagaa gtgctcacgc tggacggttt ctgcaaactg
     3781 cgtcgtgaac tggatgcgca accaactggt ttcctcgaca gccgcattcg tcgcttccag
     3841 tcatttgctg aaattagcac tgaacccggt cctcactttg gcctcggtct ggaagcatac
     3901 gccacctgga cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg tctgctgaaa
     3961 gcggttatca aaggcgaaac tgcgacgcgt ccacaggatg aaatcactgt ccaaatggcc
     4021 gagcgtcgcc gtctcaatcg gatggcagaa cgtgatgttg gtgactggtt atacgcacgc
     4081 ttcctgaaag acaaagccgg gaccgacacc cgtttcgcag cggaaattgt cgatatcagc
     4141 cgtggcggca tgcgtgttcg tttggttgat aacggcgcta tcgcctttat tcctgcacct
     4201 ttcttacacg ctgtgcgcga tgaactggtt tgcagccagg aaaacggcac cgtacaaatt
     4261 aaaggtgaaa cggtttataa agtaactgac gttattgacg tcaccattgc cgaagtccgc
     4321 atggaaaccc gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac ggcccattcc
     4381 ttatgaatgg gccgtttatt tccccgctct accttcatca tatcccggca gttttttaac
     4441 tgactttcgt ttgaaaactg gcgtttttcc aaaactagat tacaaataaa tatataaaaa
     4501 aaacaatgtg tttagccgcc gttaatttca tgtaagtcat gttgttcctt ttatttaacc
     4561 gcggactccg ctgttaaccg gaggatatgc atcatgaaaa ccgttaggga gtccacaacg
     4621 ttgtacaact ttctcggatc gcacaatcca tactggcggt tgacggaaag cagcgatgtt
     4681 ttgcgctttt ctaccaccga aaccacagaa cctgaacgta cgttgcagtt atctgccgaa
     4741 caggctgctc gcatcaggga aatgacggtc atcacctcca gcctgatgat gagtctgacc
     4801 gtcgatgaga gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa acgggaatgg
     4861 gctggcaacg cgtctgcatg gcatgacaca ccagcagttg ctcgtgattt atcacacgga
     4921 ctttcctttg ctgagcaggt agtttctgaa gcacattccg caatagtgat tctcgacagc
     4981 cgggggaata tccaacgctt caatcggtta tgtgaagatt acacagggtt gaacgaacac
     5041 gacgtcattg gccaaagcgt gtttaaactg tttatgagcc gtcgtgaagc tgcggcatcc
     5101 aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga actgtggata
     5161 ccaacacgta aaggccagcg gctgtttctg tttcgcaata aatttgtcca cagcggcagt
     5221 ggcaaaaacg agattttttt aatctgttcc ggcaccgaca ttaccgaaga gcgccgcgct
     5281 caggaacgac tgcgtattct ggcaaatacc gacagtatca ccggactgcc gaatcgtaac
     5341 gcaatgcagg agttaatcga tcacgctatt aatcaggcag ataacaataa agttggggtc
     5401 gtgtatcttg atttggataa ttttaaaaag gtcaacgacg cctatgggca tttatttggt
     5461 gaccagttat tacgcgacgt gtcattggct attttaagct gtctcgaaca tgaccaggtg
     5521 ttggcgcgtc caggtggcga tgagtttctg gtactggcat ccaacacctc acaaagcgcg
     5581 ctggaagcaa tggcatcacg aattttgacc cgcttacggc tcccctttcg cattggttta
     5641 attgaagttt ataccagctg ttcagtaggt attgcactct ctcccgaaca tggttcggac
     5701 agcgcggcta ttattcgtca cgccgacaca gcaatgtaca cagcaaagga aggcggacga
     5761 ggacaatttt gcgtttttac cccagaaatg aatcaacggg tatttgaata tctctggctg
     5821 gataccaact tgcgtaaagc actggaaaac gatcagttgg ttattcacta tcaacccaaa
     5881 atcacctggc gtggcgaagt gcgcagtctg gaagcactag tacgttggca gtcacctgaa
     5941 cgtgggttga ttccaccgtt ggacttcatt tcctacgcag aagagtcagg gctaattgtg
     6001 cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcgaagtg gcgggataaa
     6061 ggcattaacc tgcgagtggc ggtaaacatt tctgcacgtc agctcgccga tcaaaccatt
     6121 ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc tattgatgtt
     6181 gaattgacag agagttgtct gattgagaat gatgaactgg cactgtctgt tattcaacaa
     6241 tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccgccta ctcttcactt
     6301 tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt tgttcgagat
     6361 attcacaaac aacctgtctc gcagtcactg gttcgggcaa tcgtcgctgt ggcccaggca
     6421 ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc ttttttaacc
     6481 aagaatggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc cgccgtcgcc
     6541 ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata tatatactta
     6601 gcgctatatt ccgtcattat atatcgtatt cccggatgat aattagccgt tgctgttaaa
     6661 cacttctctt ttagcggcaa tttttgcatt attcattttc ccccttttgc tataaggatg
     6721 tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat tgtgttagat
     6781 atccttgtgg cagacgatta ccactctgcg atccataatc tcgagatact taaagcagaa
     6841 ctcttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg ggagatttaa
     6901 caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat ttttttattt
     6961 agtattataa cgttataaga attacagcga tgaattcccg acaacaaact attctacaga
     7021 tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact ggagtttctg
     7081 aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc cgccgtgcac
     7141 atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg agcaactata
     7201 cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc gaaaccatct
     7261 ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag cagaagaaaa
     7321 atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac gcgccttgtg
     7381 aagttatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt ggcccgttga
     7441 cacgccagtg catccaacag gtacatttca gcaaagcatt tattggtatt gatggctggc
     7501 aacctgaaac tggattcacc ggtcgcgata tgatgcgtac cgatgtggtg aatgccgtgc
     7561 tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt gctgtacatt
     7621 catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg aaaatacgcg
     7681 ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt gacatttaac
     7741 catttccctt ctctacggat gatttgcagt ttgacaaatc atccgctcta agatgattcc
     7801 tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa aattattatc
     7861 agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc gagcggattt
     7921 cacggaataa tttcaccagg cttattctta gctattatag ttatagagag cttacttccg
     7981 tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat gaccgcggct
     8041 gttctggcaa ttaccctggc aatgtctctg agtgcctgtt ctaactggtc taaacgggac
     8101 cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact gaccgatggc
     8161 agtacgttgg gtacattagg tggcgcagct gtcggtggtg ttattggtca ccaggtcggt
     8221 aaataatcgt aaataaatgt cgattatcga ctcgtaatta taatacgctt ga
//
LOCUS       DAZKFZ010000201         8257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000201 DAZKFZ010000000
VERSION     DAZKFZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.201"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            39..173
                     /locus_tag="ACHOQW_003935"
     CDS             39..173
                     /locus_tag="ACHOQW_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFP9574922.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLAGELSIVRVLNDFQPLLILLVN
                     "
     gene            complement(170..412)
                     /locus_tag="ACHOQW_003936"
     CDS             complement(170..412)
                     /locus_tag="ACHOQW_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9574923.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            544..1827
                     /gene="ydfJ"
                     /locus_tag="ACHOQW_003937"
                     /pseudo
     CDS             544..1827
                     /gene="ydfJ"
                     /locus_tag="ACHOQW_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1916..3376
                     /gene="ydfI"
                     /locus_tag="ACHOQW_003938"
     CDS             1916..3376
                     /gene="ydfI"
                     /locus_tag="ACHOQW_003938"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9574924.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3412..3615)
                     /gene="ydfZ"
                     /locus_tag="ACHOQW_003939"
     CDS             complement(3412..3615)
                     /gene="ydfZ"
                     /locus_tag="ACHOQW_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP9574925.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3792..4478)
                     /gene="rspR"
                     /locus_tag="ACHOQW_003940"
                     /gene_synonym="ydfH"
     CDS             complement(3792..4478)
                     /gene="rspR"
                     /locus_tag="ACHOQW_003940"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP9574926.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4567..5313)
                     /gene="ydfG"
                     /locus_tag="ACHOQW_003941"
     CDS             complement(4567..5313)
                     /gene="ydfG"
                     /locus_tag="ACHOQW_003941"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP9574927.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5450..7495
                     /gene="dcp"
                     /locus_tag="ACHOQW_003942"
     CDS             5450..7495
                     /gene="dcp"
                     /locus_tag="ACHOQW_003942"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP9574928.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7540..8058)
                     /gene="ydeJ"
                     /locus_tag="ACHOQW_003943"
     CDS             complement(7540..8058)
                     /gene="ydeJ"
                     /locus_tag="ACHOQW_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP9574929.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2084 a         1907 c         2065 g         2201 t
ORIGIN      
        1 aaaattaaga tagcaatggc gggttaactt ttcgtaaaat gaaattaatt ctcggtacag
       61 catgtaaaac gcggcacctc gtatgcaaga acgtgctgcg gttggctggt gaactttcga
      121 tagtgcgagt attgaatgat ttccagccgt tactgatttt actcgttaat tagcgaacaa
      181 accactcgtc agcagactcc caggtatctt tcagcgtctc ctgaacaaaa gtttttgcag
      241 aatctttatc ggcggtgcga gtaactgaaa ggccatcgtt gctggtggat ttgatgatca
      301 cttctacatc gtcataacgt ttactgacac gtcgcagcat ttcttgttgc agggcaggaa
      361 cagaaccttt tggcattttg ttgattttgg ctgcggggta tctgtggggc atatatggga
      421 cataaaaagg cctcaaataa acagaaaaat cgagattagg gatttttaga aaaaatacaa
      481 ccatctgaaa aaacttagaa aacacccaaa aacccacaga atagtaaaaa acatctatga
      541 attatggatt tccagttata ttcgctcggc gcagcgttag tgtttcatga aatatttttt
      601 cctgaatcat caacggcaat ggcgttaatt ctggcaatgg gaacctacgg tgcaggttat
      661 gtggcgcgta ttgtcggagc atttattttc ggcaaaatgg gcgacagaat agggcgtaaa
      721 aaagtgctct ttattaccat caccatgatg gggatctgta ccaccttaat tggagtgtta
      781 ccgacctatg cacagattgg tgtttttgcc cccatcttgc tggtgacgct gcgtattatt
      841 cagggattgg gtgcaggtgc ggaaatttcc ggtgccggta cgatgctggc ggaatatgcg
      901 ccaaaaggta agcgcggaat tatctcctca tttgtggcta tgggaactaa ctgcggaacc
      961 ttgagcgcaa cggcaatctg ggcctttatg ttcttcattc tcagtaaaga ggaactgctg
     1021 gcgtggggat ggcgtatacc gttcctggcg agcgttgtcg tgatggtctt tgctatctgg
     1081 ttgcgtatga atctgaaaga aagcccggtc tttgagaagg ttaacgacag caaccaaccg
     1141 acagcaaaac ctgcacctgc tggtagcata ttccagagca aatccttctg gctggcaaca
     1201 gggctgcgtt ttggtcaggc aggtaactcc gggttaattc agactttcct tgcaggctat
     1261 ttagtacaga cgttattgtt taacaaagca attccaacag atgcattgat gatcagttcg
     1321 attcccggct ttatgaccat tccgttcctt ggttggttat ccgataaaat tggtcgccgg
     1381 atcccgtata ttattatgaa tacctccgcg attgtgctgg catggccaat gctttctatc
     1441 atcgtagata aaagctatgc cccgagcacc attatggttg cactgattgt gattcataac
     1501 tgtgcggtgc tgggattatt tgctctggaa aacattacca tggcagaaat gttcggctgt
     1561 aaaaaccgct ttacccggat ggctatttct aaagaaattg gtggtcttat cgcttccggt
     1621 tttggtccta tcctggcggg tattttctgc accatgacgg aatcctggta tccgatcgcc
     1681 attatgatca tggcatattc agtgattggt ttaatctctg cgctgaaaat gccagaagtg
     1741 aaagaccgtg atttaagtgc gctggaagac gctgcggaag atcaaccgcg tgttgtaaga
     1801 gctgcgcaac cttccagaag tctgtaaatc ctcaatccct tctcttaccg gagaggggat
     1861 ttttattcat ataacaaaac atgtagcttg ccatatttaa atttaaggaa ttgttatggg
     1921 aaataatttg ttatcagcaa aagcgacact ccctgtttat gatcgtaata acctggcccc
     1981 aagaattgtt catttaggct ttggtgcatt tcaccgtgcg catcagggcg tgtatgccga
     2041 tattcttgct acagaacatt tcagtgactg gggatattat gaggtcaact taatcggcgg
     2101 cgaacagcaa attgccgatt tacatcagca agataatctt tataccgttg cggaaatgtc
     2161 ggccgatgcg tggacggctc gcgtcgttgg cgtcgttaaa aaagccttgc acgtacagat
     2221 agatggctta gaaaccgtgt tggctgcgat gtgtgaaccg caaatcgcga ttgtctctct
     2281 gacaatcacc gaaaaagggt atttccactc tccggcgacc ggacagttaa tgctcgatca
     2341 cccgatggtc gctgccgacg tgcaaaatcc ccaccagccg aaaacagcaa caggggtgat
     2401 tgttgaggcg ctggctcgcc gtaaagcggc aggacttccc gcatttaccg tcatgtcatg
     2461 tgacaacatg ccagaaaacg gtcatgttat gcgtgacgtt gtcacttcct acgcacaagc
     2521 cgttgatgta aaactagcac aatggatcga agataacgtg actttcccat caacaatggt
     2581 ggaccgtatt gtgcccgcag tgacagagga tacgctggcg aaaatcgaac agcttaccgg
     2641 tgtgcgcgat cctgctggcg ttgcctgtga acctttccgc cagtgggtaa tcgaagataa
     2701 ctttgttgcc ggacgtccgg aatgggaaaa agcgggagcc gaactggtta gtgatgtgct
     2761 gccttatgaa gagatgaagt tgcgcatgct caacggcagt cattcattcc tggcgtatct
     2821 gggttatctt gccggatatc agcacattaa tgactgtatg gaagatgaac attatcgtca
     2881 tgcggcgtat gccttgatgt tgcaggaaca agcgccgacg ctgaaagtgc agggcgttga
     2941 tttgcaagat tacgctaacc gattaattgc acgctatagc aacccggcgt tacgtcatcg
     3001 aacctggcag attgcgatgg atggcagcca gaaattgcca cagcggatgt tggattctgt
     3061 tcgctggcat ctggcgcatg acagcaagtt cgatctgctg gcgctgggcg tcgcgggttg
     3121 gatgcgttat gtcggtggtg ttgatgaaca gggaaatccg atagaaatca gtgacccact
     3181 gttacctgtt attcagaagg ctgtacaaag tagtgccgaa gggaaagcgc gcgtccagtc
     3241 attgctggcg attaaggcga tttttggtgg tgatttgcca gacaatagct tgtttactgc
     3301 aaaagtgacg gaagcgtact tgtctttatt agcgcatggt gcgaaagcga ccgtggcgaa
     3361 atattccgtg aagtaaaaaa agcaatgccg ccatcggcgg cattcacacg cttagttcat
     3421 gccgagacga atcaggtcca gtggtgccag tttctcttca caaccttcaa caactacggt
     3481 ttttccgcgg cggatttgct cagcggtcag accttcagta tcaatcgaca ggatcttgcc
     3541 agtgtgaccg gtgccgctaa ccataacacg gctgccagtg gtgattgcgt tacggttacg
     3601 atcgtaggtc gtcataggtg ttttctcctt tctgatttac agtcgaagcc cacgtaacgg
     3661 gcgcaatata aatactcctg tcgagtgaaa tattttttgt ttttgatcaa gctcacatct
     3721 tttttgatgc atgcatgagc cataaggcgg gggaaaagac tctcaccccg gatgggatga
     3781 gaggaaatga attactcttc gctaaaccag tcgctatttt cctggcggat ctggcgcacg
     3841 gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt
     3901 ttttgcaggg cagagaatat atcaagatgc tggcgtaaca gcatttctgg tggagaaaca
     3961 tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc aatggtatcc
     4021 cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc atcaagttca
     4081 aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc
     4141 agttggtagc actggctttc ggtaatcata ctcgccgccc gacgcgccac cgcgcactca
     4201 atggcctgac ggataaaact gccgttgcgc acctgggcca tggaaatttt gttgacgtag
     4261 ctgccacgct gcggacgaat ttgaatcagg ccgttttccg ccagtttaat aaaggcttca
     4321 cgaaccggct ggcgtgacac attgaaacga acagaaactt ctttttccga caacggtgtg
     4381 cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa tacgataaat ctgctgattg
     4441 acaggctgtg tgggattaag ttgcgtttcg acggtcattc gttcttgctt gtgagtgagt
     4501 taactgcatg agtctaccac ttttttgtga caagcaataa cccggcagtt acaccgggta
     4561 taaaaattac tgacggtgga cattcagccc ggcatagctt tgggtaaccg gcatcatttc
     4621 cagggtattg atattgacgt gagcaggcag cgttgacacc caccagacgg cttcgctgac
     4681 atcttctggc gttaatgcaa cggtattttg ataggttttt tccgctttac cgtcatcgcc
     4741 tttaaagcgg acattggaaa actcggtgcc acccaccaga cccggttcga tgtcggtgac
     4801 gcgcaccgcc gtaccatgca ggtccgtacg cagattcagg ctaaactgac gaacaaacgc
     4861 tttcgtcgca ccgtaaacgt taccaccggc atacggccag ctacctgccg ttgagccaat
     4921 gttaataata tgaccatgat tacgttcaac catacccggt aagacggcgc gcgtcatata
     4981 taccaggcct ttgttgttgg tatcaatcat cgtttcccag tcttcaatgc tggctttatg
     5041 cgcaggctcc atgcccaacg ccagaccggc attatttacc aggatatcaa tattgcacca
     5101 ctcggcagga agcgatgcca gcatctcttc aatagcggcg cggttgcgaa cgtccagttg
     5161 ggcgatgtac agattatctc ccagttcgtc ttttaactcc tgcaaccgtt cctggcgacg
     5221 gccagtggcg ataactttat gcccttgttg aataaaacga cgagtaatgc attcaccaaa
     5281 acctgccgtt gctccagtta ctaaaacgat catctcactg ttcctcaacg cttttgtgtg
     5341 tcaataacat agcacgtgat gcttaagagc gggtaatact ggtttgcaag attgcactcc
     5401 gttatcagta agcctacgct atggaacacc tcgcgttcag gagaagaaaa tgacaacaat
     5461 taatcctttc cttgtgcaaa gcacactgcc gtatctggct cctcattttg atcaaattgc
     5521 caatcatcac tatcgcccgg cattcgatga gggaatacag caaaagcggg cagaaattgc
     5581 tgccatcgcg cttaacccgc aaacacctga tttcaacaat actattctgg cactggaaca
     5641 aagcggagaa ttacttaccc gcgttaccag cgtctttttt gcgatgactg cggcgcatac
     5701 caatgatgaa ttacagcgtc ttgacgagca gttttccgct gaactggcgg aactggctaa
     5761 tgatatctat ctgaacggtg aattattcgc gcgggtagat gctgtctggc aacgccgtga
     5821 atctctgggg cttgatagtg aatccatccg cctggtggag gtgattcatc aacgttttgt
     5881 cctagccgga gccaaacttg cacaagctga taaagcaaaa ttaaaagtac tgaatacaga
     5941 agctgcgaca ctgaccagtc agtttaacca gcggttactg gcagcaaata aatccggcgg
     6001 tctggttgtg aacgatatcg cgcagctggc aggaatgagt gagcaagaga ttgcgctggc
     6061 ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg ctgattccgc tgctgaatac
     6121 cacccaacaa ccggcgcttg ccgaaatgcg cgatcgtgcg acgcgtgaaa aactgtttat
     6181 tgcgggctgg acgcgagcgg aaaaaaatga tgccaatgat acccgcgcta tcattcaacg
     6241 tctggtggag atccgtgcac aacaggctac attactcggt tttcctcatt atgccgcatg
     6301 gaaaatcgcc gatcagatgg caaaaacacc tgaagcagca ctcaacttta tgcgggaaat
     6361 tgttccagcg gcgcgtcaac gtgcgagcga tgaattagcc tccatacagg cggttattga
     6421 taagcagcag ggcgggttta gcgcgcagcc gtgggactgg gcattttatg ccgaacaggt
     6481 ccggcgagag aaatttgatc ttgatgaggc gcagctcaag ccatattttg aattaaacac
     6541 ggtgttgaat gaaggtgtat tctggaccgc gaatcagctc ttcggtatta agtttgtcga
     6601 acgttttgat attcctgtct accatcctga cgttcgggtg tgggaaattt ttgatcataa
     6661 tggcgtgggg ctggcgttat tttacggtga tttcttcgcc cgtgattcaa aaagcggcgg
     6721 tgcatggatg ggcaattttg ttgagcaatc aacgcttaat gaaacgcatc cggtaattta
     6781 taacgtctgc aattatcaga aacccgctgc cggtgagcct gcgttgttac tctgggatga
     6841 tgtcataacc ttattccatg aatttggtca tacgctgcac ggcctttttg cccgccagcg
     6901 ttatgccacg ctctccggca ccaacacgcc gcgtgatttt gtcgaatttc cgtcgcaaat
     6961 caacgaacac tgggcaacgc atccgcaggt attcgctcgc tacgcccggc attatcagag
     7021 cggggcagca atgcctgacg aactgcaaca gaaaatgcgt aatgccagcc tgttcaacaa
     7081 agggtatgag atgagcgaac tgcttagcgc cgcacttctc gatatgcgct ggcattgcct
     7141 ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa ttgcgggcgc tggtggcaga
     7201 aaatatggat cttcctgcta taccgccacg ctatcgcagc agttattttg cccatatttt
     7261 tggtggcgga tatgccgcag gttattacgc ttatctgtgg acgcaaatgt tggccgatga
     7321 tggttaccag tggtttgttg agcagggcgg attaacgcgt gaaaatgggc tgcgttttcg
     7381 cgaggcgatc ctttccagag gtaacagcga agatctggaa cgcctgtatc gacaatggcg
     7441 cggtaaggct cctcagatta tgccgatgct gcaacatcgt ggcttgaaca tataaacacc
     7501 accaatggct gtcctggaga tatcgccagg ccagcctggt tatagcagta actgcagcag
     7561 ctgggcgagg gcaaacctca ccgctaaagc taataccgtt tcgcagtcgc cagcaaaatg
     7621 cataaccgca gtgtagttct ggcctttaat atgccacgcg aaccagacgg tacccgctgg
     7681 cgtaccatct tcaccgccct ccggtccgcc gtagccggta atggcaatac tgacatcagc
     7741 atccgcacgc tctatggcac cggttgccat ttctgctgcc actttctcac tcaccgcaga
     7801 atatcgttca agagattgct ggcttacgct gaggattttc atctttgcct gatcggtgaa
     7861 agtaacaaag cctgcaccgt aaaatttggg tgtatcttca gctgcacaca gggcgctagc
     7921 caacttaccg ccggtgcatg attcggcggt agttaagcgt aattgatccg cgataagtct
     7981 ttgactcaac gcggatgtca ggttttcaat agtgtcggta tctgcaagtt gtacaatttt
     8041 atttctatta atgttcactg ttttttgtcc acgcttttat atgtaaaggt gatttgcttt
     8101 ttctgtatgc tcgtccaata taataatttt caaacatata aatccgatta ttctgaaaat
     8161 atgtttaaga ctcttcaatc ttcttaactt tagtgagtta aattctctga atatttgaca
     8221 gctagttggc tgattttaaa tgccttataa caattgg
//
LOCUS       DAZKFZ010000202         8247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000202 DAZKFZ010000000
VERSION     DAZKFZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.202"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..106
                     /locus_tag="ACHOQW_003944"
     CDS             <1..106
                     /locus_tag="ACHOQW_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574930.1"
                     /translation="TNDKVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            372..2609
                     /gene="nrfB"
                     /locus_tag="ACHOQW_003945"
     CDS             372..2609
                     /gene="nrfB"
                     /locus_tag="ACHOQW_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP9574931.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2596..5568
                     /gene="nfrA"
                     /locus_tag="ACHOQW_003946"
     CDS             2596..5568
                     /gene="nfrA"
                     /locus_tag="ACHOQW_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP9574932.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5569..6459
                     /gene="ybcH"
                     /locus_tag="ACHOQW_003947"
     CDS             5569..6459
                     /gene="ybcH"
                     /locus_tag="ACHOQW_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP9574933.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6642..7403
                     /gene="envY"
                     /locus_tag="ACHOQW_003948"
     CDS             6642..7403
                     /gene="envY"
                     /locus_tag="ACHOQW_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP9574934.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7449..7525)
                     /locus_tag="ACHOQW_003949"
     tRNA            complement(7449..7525)
                     /locus_tag="ACHOQW_003949"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7489..7491),aa:Arg,seq:tct)
     gene            7768..>8247
                     /gene="fimZ"
                     /locus_tag="ACHOQW_003950"
     CDS             7768..>8247
                     /gene="fimZ"
                     /locus_tag="ACHOQW_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP9574935.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     "
BASE COUNT         2051 a         2128 c         2108 g         1960 t
ORIGIN      
        1 gacgaatgat aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga
       61 cagaaactat ctggcgtcag tctttgcggg gagcgggctt tcgtaatctt gccctgcctg
      121 ctcacctcca ggcacttcca ctacatcatg acctgatcgg tgccaccact cctcttcact
      181 atgacgttaa cctcccctcc ttgacaccac gcttttgtct acattctacg caaacggcaa
      241 cttttataac cagtgtaaaa ataacgtgcc gcaatagtca tacctattaa tggtaaaaag
      301 ctgtcacaat tcataaaaaa ccttaatata cgccacccta aacataacca gcgttaatgt
      361 aaggtttttg tgtggactgg cttcttgatg tttttgctac ctggctctat ggcttaaaag
      421 taatcgcgat aacgttagcg gtcatcatgt tcatcagcgg gctggacgat ttttttattg
      481 atgtcgtcta ctgggtacgc cgcattaaac gcaagttgag tgtttatcgc cgctacccgc
      541 gaatgagtta ccgcgaactg tataaaccag atgaaaaacc gttagcgatt atggttccgg
      601 cgtggaatga aacgggcgtc atcggcaata tggccgagct ggcggcgacc acgctcgact
      661 acgaaaacta tcatatcttt gttggcacct accccaacga cccggacact cagcgtgatg
      721 ttgacgaagt gtgcgctcgc ttcccgaatg tgcataaggt agtctgcgcg cgtcctggcc
      781 ccaccagcaa agccgactgt ctgaacaacg tgctggacgc catcacccag tttgagcgta
      841 gcgccaattt cgcttttgct ggttttattc tgcatgacgc cgaagatgtg atttcaccga
      901 tggaattgcg tctgttcaac tatctggtcg agcgtaaaga tctgattcag atcccggtgt
      961 atccgtttga acgcgaatgg acgcacttca ccagcatgac ttacattgat gagttttcag
     1021 agctgcatgg caaagatgtt ccggtgcgtg aagccctcgc cggacaagtg cccagcgcag
     1081 gcgtcggcac ctgtttcagc cgccgcgccg tgaccgctct gttagctgac ggtgacggta
     1141 ttgctttcga cgtgcagagt ctgactgaag attacgacat tggcttccgc ctgaaagaaa
     1201 aaggtatgac ggaaattttt gtccgttttc cggtggtgga cgaagccaaa gaacgcgagc
     1261 agcgtaaatt tttacagcac gcacggacgt caaacatgat ctgcgtgcgc gaatatttcc
     1321 ccgatacctt ttcgactgcg gttcgacaaa aatctcgctg gatcatcggc attgttttcc
     1381 agggctttaa aacccataaa tggacctcca gcctgacgct gaactacttt ctctggcgcg
     1441 accgcaaagg ggcaatcagt aactttgtca gcttcctcgc aatgctggtg atgatccagc
     1501 ttttgctgtt gctggcgtat gaaagtttgt ggcccgatgc ctggcatttc ctttctattt
     1561 ttagcggcag cgcatggtta atgaccctgc tgtggctaaa ctttggcttg atggttaacc
     1621 gcatcgtgca gcgggtgatt ttcgtcactg gctactacgg cctgacgcag gggctactat
     1681 ctgtcctgcg tcttttctgg ggcaacctga ttaactttat ggccaactgg cgcgcgttaa
     1741 aacaggtact tcaacacggc gatccacgtc gcgtcgcgtg ggataaaaca acgcatgact
     1801 tccccagcgt cactggcgat acccgctcgt tgcgcccgtt aggtcaaatt ctgctggaaa
     1861 atcaggtcat cactgaagaa cagctcgata cagcactgcg taatcgcgtc gaaggtctac
     1921 gcctgggcgg ttcaatgctg atgcaggggc tgattagcgc cgagcagctg gcacaggcgc
     1981 tggcagagca aaacggcgtg gcgtgggaat ccatcgatgc ctggcagatc ccgtcctcgc
     2041 tgattgccga aatgccggcc tccgtggcgc tgcattatgc ggtactgccg ctgcgtctgg
     2101 aaaatgatga gttaattgtc ggcagtgaag atggtattga cccggtttcg ctggcggccc
     2161 tgacgcgtaa agtcggacgc aaagtgcgtt atgtcattgt tctgcgggga caaattgtca
     2221 cggggttacg tcactggtat gcacgccgac gcggtcacga tccgcgggca atgttgtaca
     2281 atgcggttca gcatcagtgg ctcacggaac agcaggccgg tgaaatctgg cggcaatatg
     2341 tgccgcatca gttcctgttc gccgaaatac tgaccacgct cggtcatatt aatcgttcag
     2401 caattaacgt gttgttattg cgccatgaac gcagttctct gccgctcggc aagtttttgg
     2461 tcaccgaagg cgttatcagc caggaaacgt tggatcgcgt cctgacaatt caacgcgaat
     2521 tacaagtttc gatgcaatca ctattactca aagcaggttt aaacacagaa caggttgcgc
     2581 aactggagtc cgaaaatgaa ggagaataac cttaatcgcg tcatcggatg gtctggttta
     2641 ctgctgacgt ctttattgag taccagcgca ctcgcagaca atatcggcac cagcgcagaa
     2701 gagctggggc tgagcgatta tcgccatttt gttatttatc cccgtctcga taaggcgctg
     2761 aaggcacaga aaaataacga cgaagcaacc gccatccgcg aatttgaata tatacaccag
     2821 caggtgccgg ataatattcc gctgacttta taccttgcgg aagcctatcg ccattttggt
     2881 catgatgacc gggcacggct gttgcttgag gatcaactga aacgtcaccc aggagatgcc
     2941 cgacttgagc gcagtctggc ggctattccg gttgaagtga aaagcattac aactgttgaa
     3001 gaactgcttg cccagcaaaa agcgtgcgat gctgcgccga ccctgcgttg tcgcagtgaa
     3061 gtcgggcaga atgccctgcg gctggcacag ttacctgtcg ccagagcgca actgaacgac
     3121 gcgacgtttg ctgcatcgcc ggaaggaaaa acgctgcgaa ccgatctgct gcaacgggca
     3181 atctacctga aacaatggtc ccaggcagat acgctataca atgaagcacg ccagcagaac
     3241 acattaagcg cggcagaacg ccgtcagtgg tttgacgtgc ttcttgccgg gcagctggac
     3301 gatcggatcc tggcactgca atcacagggg atcttcaccg atcctcagtc atatattact
     3361 tacgcgaccg cgctggctta tcgtggcgaa aaagcacgcc tccagcatta tctcattgaa
     3421 aataagccgc tgtttaccac ggacgcacaa gagaaaagtt ggctctatct gttatctaaa
     3481 tacagcgcca accccgttca ggcattggcg aattatacgg tgcagtttgc cgataaccgc
     3541 cagtatgttg ttggcgcgac gctaccggtg ctgttaaaag aaggtcagta cgacgcagcg
     3601 caaaaactgc tcgccaccct ccccgccaat gaaatgcttg aggagcgtta tgctgtcagc
     3661 gtggcgaccc gtaacaaggc tgaagctctg cgtctggcac gcttgctgta tcagcaagaa
     3721 tcggcaaatc ttacccgcct ggatcaacta acctggcaac tgatgcagaa cgagcagtca
     3781 cgggaagctg ccgatttatt gctgcaacgc tatcctttcc agggcgatgc gcgtatcagc
     3841 cagactttaa tggcgcgact ggcgtctctg ctggaaagtc atccttacct ggcaacgccg
     3901 gcgaaggtgg cgattttatc gaaaccctta ccgctggcgg agcaacgtca gtggcaaagt
     3961 cagttgccgg gtattgcaga taattgcccg gcaatagttc gcttgctggg cgatatgtcg
     4021 ccttcctacg atgccgccgc ctggaaccgt ctggcaaagt gttatcggga cacgctaccc
     4081 ggtgtggcgt tgtatgcatg gcttcaggcc gaacaacgac aaccgaacgc ctggcaacat
     4141 cgtgcggtag cctatcaggc gtatcaggtt gaggactacg ccaccgcact ggcggccagg
     4201 cagaaaatca gtcttcacga catgagcaat gaggatctgc ttgctgctgc caataccgcc
     4261 caggcggcag gaaatggtgc ggctcgcgat cgctggctac aacaggcaga acaacgtgga
     4321 ctgggaagca atgccctcta ctggtggctg catgcgcaac gttacattcc tggtcagccg
     4381 gaactcgcac tgaacgatct cacgcgctca atcaatattg cgccttctgc caacgcttac
     4441 gttgcgcggg cgacaattta tcgccaacgt cataatgtcc cggccgcggt gagtgatttg
     4501 cgcgccgcgc tggaactgga accgaataat agcaacaccc aggcagcgct tggttacgcc
     4561 ttgtgggata gcggtgatat cgcacagtcg cgggaaatgc tcgaacaggc gcataaaggg
     4621 ctaccggacg atccggcact gatccgacaa ctggcctatg tgaaccagcg tctggatgac
     4681 atgcctgcga cgcagcacta cgcccggctg gtgattgatg acattgataa tcaggcgctg
     4741 ataaccccac tgaccccaga gcaaaatcag caacgcttca atttccgccg tctgcatgag
     4801 gaggtcggtc gccgctggac gttcagtttc gattcttcca tcggcttgcg ttccggggca
     4861 atgagtaccg ctaacaataa tgttggcggc gcagcgccag ggaaaagcta tcgtagctac
     4921 gggcaactgg aagccgaata ccgcatcgga cgcaatatgc tgctggaagg cgacctgctc
     4981 tcagtttata gccgcttctt tgccgatacc ggagaaaacg gggtgatgat gccggtgaaa
     5041 aatccgatgt ccggcaccgg tctgcgctgg aagccgctgc gcgatcagat ctttttcctc
     5101 gccgtcgaac agcagttgcc gctgaacggg caaaatggcg catccgatac catgctgcgc
     5161 gccagcgcct cattctttaa tggcggcaaa tacagcgacg aatggcaccc gaacggttca
     5221 ggctggtttg cccaaaacct gtacctcgat gcggcgcaat atatccgcca ggatattcag
     5281 gcgtggacgg cagattatcg cgtcagctgg catcagaagg tagctaacgg acagactatt
     5341 gagccttacg ctcacgttca ggacaacggc tatcgtgata aaggcactca gggcgcgcag
     5401 cttggcggtg tcggggtccg ctggaatatc tggaccggcg agacgcacta cgacgcctgg
     5461 ccgcacaaag tcagtctcgg cgtcgagtat caacatacct ttaaggcgat taatcaacgt
     5521 aacggagagc gcaacaacgc gtttctcacc attggagtgc actggtaaat gcgtaagttc
     5581 attttcgtat tgctgacact gcttttggtc agcccttttt cctttgcgat gaaaggtatt
     5641 atctggcaac cacaaaaccg tgatagtcag gttaccgata cccagtggca ggggctgatg
     5701 agtcagttac gtttgcaagg cttcgatacc ctggttttgc aatggacccg ttacggcgat
     5761 gcattcaccc agccagaaca gcgcgcgtta ttgtttaagc gggccgcagc tgcgcaacag
     5821 gctggcctga agctgattgt cgggctgaac gccgatccgg aattttttat gcaccagaaa
     5881 cagtcatccg cggcgctgga aagctatctt aatcgcctgc tggctgccga tctccagcaa
     5941 gccagattat ggagtgccgt acctggcgta acgccggatg gctggtactt cagcgctgaa
     6001 attgacgacc tgaactggcg cagcgaagcc gcccgtcagc ctttgctaac atggttaaac
     6061 aacgcgcagc ggctgattag cgatgtttca gcaaaaccgg tttatatcag tagttttttc
     6121 gccggaaaca tgtcgccaga tggctatcgc caactgctgg aacacgttaa agcaaccggc
     6181 gttaatgtct gggtacagga tggcagcggc gtggataaac ttaccgctga acagcgtgaa
     6241 cgttatctac aggccagcgc cgattgccaa agctccgccc cagcaagcgg cattgtttat
     6301 gaactgtttg tcgccggcaa aggcaaaacc tttacagcga aaccgaaacc ggacgcagaa
     6361 attgcctcgc tgttagcgaa acgttcctct tgcggtaaag acactctcta tttctctctg
     6421 cgctatttgc ccgtcgcgca cggcattctc gagtattaaa tctcctccag gtaagtcggg
     6481 tacgacctgg cttacctctt tcgctctcgt cagaattcca tcaagattaa ttgctaaaaa
     6541 gcgtgctaaa gaaagaagat cgttatcggc gaacgggccg atcactcatg caggaagcat
     6601 agatcctttg agcggagttt ctgatatctg aggagtgcga aatgcaattg agcagcagtg
     6661 aaccttgcgt ggtgatcctg accgaaaaag aggtagaggt aagcgtcaat aaccatgcta
     6721 cgtttaccct tccgaaaaac tacctggccg ccttcgcgtg caacaataac gtcattgaac
     6781 tctcaacgtt aaatcacgta ttaatcaccc acatcaaccg taacatcatc aacgattatc
     6841 tgttgttttt aaataagaac ttaacctgtg taaagccctg gtcgcggctg gctaccccgg
     6901 ttatcgcttg tcatagccgt acaccggaag tgttccggct agccgccaac cacagcaagc
     6961 agcaacccag caaaccctgc gaagcggagt tgacgcgcgc attgcttttt accgtattgt
     7021 ctaactttct tgagcaatcg cggtttattg ccctactgat gtatatctta cgcagcagcg
     7081 tccgcgacag cgtctgccgc attattcaaa gcgatattca gcattactgg aatctgcgaa
     7141 ttgtcgccag ttcgctatgt ttaagcccca gcctgctcaa aaagaaatta aaaaacgaaa
     7201 ataccagcta tagccagatt gtcacagagt gtcgtatgcg ttacgccgta cagatgttat
     7261 tgatggataa caaaaatatc actcaggtgg cgcaattatg tggctatagc agcacgtcgt
     7321 actttatctc tgtttttaag gcgttttacg gcctgacacc gttgaattat ctcgccaaac
     7381 agcgacaaaa agtgatgtgg tgaagggcaa agcggaaacg gataagacgg gcataaataa
     7441 agaagaaatg gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg
     7501 ctctatccaa ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta
     7561 tacggtcaac ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc
     7621 gcgtctgatt ttctgattta tttcgctata gcggcaaaca aacgcacacc gctgcgcgtc
     7681 tgaatcaaga aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa
     7741 ttgtccgctc ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact
     7801 catcctatca tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt
     7861 gtcctgaaaa cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat
     7921 ttaatcatta tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc
     7981 aaacaaatcc agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat
     8041 gctggcagag cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat
     8101 gatatttttc atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg
     8161 cttaactata taaaaagcaa taaatgtagt acgaatagtt caacggtcac tgtgctatct
     8221 aatcgtgaag tgaccatatt acgttat
//
LOCUS       DAZKFZ010000203         8240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000203 DAZKFZ010000000
VERSION     DAZKFZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(65..580)
                     /gene="ogt"
                     /locus_tag="ACHOQW_003951"
     CDS             complement(65..580)
                     /gene="ogt"
                     /locus_tag="ACHOQW_003951"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9574936.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(591..2117)
                     /gene="abgT"
                     /locus_tag="ACHOQW_003952"
     CDS             complement(591..2117)
                     /gene="abgT"
                     /locus_tag="ACHOQW_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP9574937.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(2154..3599)
                     /gene="abgB"
                     /locus_tag="ACHOQW_003953"
     CDS             complement(2154..3599)
                     /gene="abgB"
                     /locus_tag="ACHOQW_003953"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP9574938.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(3599..4909)
                     /gene="abgA"
                     /locus_tag="ACHOQW_003954"
     CDS             complement(3599..4909)
                     /gene="abgA"
                     /locus_tag="ACHOQW_003954"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP9574939.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            5085..5993
                     /locus_tag="ACHOQW_003955"
     CDS             5085..5993
                     /locus_tag="ACHOQW_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9574940.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            6323..6886
                     /gene="smrA"
                     /locus_tag="ACHOQW_003956"
     CDS             6323..6886
                     /gene="smrA"
                     /locus_tag="ACHOQW_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP9574941.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6907..8139)
                     /gene="dgcM"
                     /locus_tag="ACHOQW_003957"
     CDS             complement(6907..8139)
                     /gene="dgcM"
                     /locus_tag="ACHOQW_003957"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP9574942.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
BASE COUNT         2106 a         2080 c         2061 g         1993 t
ORIGIN      
        1 ccttaagttg ttaagggtaa ctttacataa aagtgtgaac aagctggcac aaattgttta
       61 atgtttacag caaaagataa ccttcgtggc gcaataacca ctcttttcgc tgaactccgc
      121 ctgcatatcc ggtcatggtg ccgtttcggc caataacccg atggcaaggt acgacgatgc
      181 tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc cgcgccagga cggcccaatt
      241 gctcagccag ttcgccgtaa tgcattacct gcccgcaggg gatagtccgt agtgttttcc
      301 agacttcgcg ctgaaatggc gtcccccccg tagcagtggg aagcgtatca ataatgctaa
      361 gattaccggc aaaatattca cgaagcttgt cgcttaaacc gcctggattg gtggcagaaa
      421 tgcgctcata gccttctttg cgataatgga tgtccagcag ctgcaccatg cgttcgctgt
      481 actcttccca ttcaaccgcc cgcaggcgaa attgctcatc gcaaatcacc cacagtggac
      541 ccagtggtgt ggcaattttt tcttcaagta atctcagcat ccgttctctc ttaagacaaa
      601 cgtgggtaaa tacccggacc tatcggcagg cccacaagat accacgccag caacatcagc
      661 agccatacca ccaaaaagat aagcggatag ggcaagacta acgaatagta agtacccagt
      721 ttcgcgtctg gtttgtagcg ttgcaggaat ccaagaaaca gtggaacaaa aggagatact
      781 ggcgctaaag gcaatacgga tgagtcggca atacgaaaga ggatttgcgc aaatgccggg
      841 tgaaagccaa gtagcataaa cattggtacg aaaatggggg ccagaatcga ccagattgcg
      901 gaaccgctgg cgataaacat gcataagaaa gaggaaagca acgccagacc gacaaacgcc
      961 gggatgccgc taagccctga actttccagg atatcggtca gccccacggc gatgaatttc
     1021 cccatgttgc tccagttaaa catggcgaca aactgggcga ggggaaaaac catcacgata
     1081 aatcccgcca tctctttcat cggttcaatc attaaatgcg gtaaatccgc ctgacgtcga
     1141 attgtgcggg tagcgatgcc ataagccagc gagacaacaa agaaaaaaag aatgatcagt
     1201 ggcacgatac ctttaataaa gggtgatggc atcacggtgt gattaatcgg atcgcgcaat
     1261 atcccgtttt ccgggatcac catcagcgca atcgcagcaa taaaaagtag cgatacgaca
     1321 cctgctatgc gtaaaccaaa acgctgactt tcggtcaatg tctgcagttt ctcatcgctg
     1381 tttccctgcc attgacctaa ccgtggctcg atgattttgt cggttatcag gccgccaaca
     1441 atcgtcagta cgactacgga gctggccata aaataccagt tatcaattac actgacgtgc
     1501 atttgcggat tgaacgcagc tgccgcttcc gtgctgatcc ccgacagcaa cacgtcggtt
     1561 gtgacaatca gtaaattagc cgtaaagccg caacctacgc ctgcaatggc agccagtaaa
     1621 cctgcaactg gatgcctgcc caccgccaga aaaatcagcg cacccatcgg tggcatgatc
     1681 actaacgccg catcggaaga aatgtggctg aaaaaagcaa taaacagcac catataactg
     1741 gcgtagcggg cattaacatg cgatgccatt ttaaccatta gtgctggcag taagccgacg
     1801 cgctccgcca gaccggcacc taaaaccagc gccaggatcg caccaagtgg agcaaaaccg
     1861 ctaaagtttt taataacatt gggtaaaaac cagtgtaatc cttccacact gagcaggttt
     1921 ttcaccacca ccggcgtacc atcggtcggg tttttcgcac tgacgccaaa ggccgacaaa
     1981 attgccgtcg tcaccatgag tacgataatc aaatagataa agagcaaaaa aggatgggga
     2041 accttgttac caattctttc gacccagcca tagagcttcc cggattggga ggacgacggt
     2101 atggatgaca tactcatggg cattcctcgg ttgttgtgtt tgcgttgttg ttgttatttt
     2161 aaaggtgacg gtgtcacgtt tttcgggata gggcagtgat acggttgcgt gtccgtaact
     2221 tgctgatgct cttgttggca ttcttgcaat agccctgaat caatgaagag attgagtgtg
     2281 gttgctgcca tagttttcgc cgccagcagc attcctttat gagcaataga tgttcgcccc
     2341 tggctaacca gttgccacgt atgtaggggt gtaccgacgg caaaacaggg gctgaaacac
     2401 tgggcaacag gcagtttcca actgacgtcg ccgacatcag tcgatgccgc aagcacgtta
     2461 tcggtggcgg catatggagc gacttcattc gccagtaccg tttcacgatg acgtagtgca
     2521 aaaaccttgc cgttttcgcc accggttgcg gcgatattat tcagactgtt ttgccgatcg
     2581 ttggaggtaa gcgtagcctg aatttgtttc gcaaaagcca gttcttcgga gttccattcc
     2641 ggggtaccaa aatgggatag ggcctggtac atggcatttt ctaaggtgcg attcgggaga
     2701 taactggaac aggctttgtc gaagcggcat tcaaccgtgg tttcggtcat caatgccgca
     2761 ccttcggcga ttttggcgac ccgatcataa atatgctgca cgtcggtcat ttcgggggcg
     2821 cggataagat aaagcacttc tgcctgcgcc tggaccacgt tgggcgagat cccgccacta
     2881 tttgtgatgg catagtgtac gcgcgctttt tcaataatat gttcgttgag gaagttggtg
     2941 ccagtggtca tcaacgttac ggcatcaagg gcgctgcgtc ccaaatgagg ggaattcgcg
     3001 gcatgtgctg cgatcccttt aaagcgccat gatgcctgaa tgtttgccag cgtacgggta
     3061 ttgaacatac cggcaaaggc ttccgggtgc caggtgagtg ccgcatccac atcatcaaat
     3121 accccctcgc ggaccatgaa cgttttaccc gagccgcctt cttcgccagg acaaccataa
     3181 aagcgcactg tgccgccttg cccatattgt tccagccatt tcttgatggc tattgcagcg
     3241 gcaaaggcgg cggttcccag caaattgtgt ccgcaaccgt gaccattttc accgggcgtc
     3301 acggatgtag gttgcgcgca acctgcttgc tgacttaaac ctgccagggc gtcatattct
     3361 cccagcaggg cgataaccgg tttgccttga ccaaacgaag caataaaggc atttgggata
     3421 ttgcctacgt tgcgggtaac ggtgaagcct gcagattcca gcgccgaagc cagatgctcc
     3481 gctgaccaga actcttcaaa acgtgtttct ggatgatccc agatttgatc ggcaatatcg
     3541 gtataacgtt ggcgatcggc ttcaatcgca tcgtcgataa aacgatagat ttcctgcatc
     3601 agatacctcg cgtccaggga aaattgagcg cggtgcgcgc cagcgtttcg acggcaatag
     3661 cgagaacctg ctcgtcaaaa tcgaattttt cgttgtgatg acctgccgcc agctgtgtgc
     3721 caaacaccac gtaggaggct tgcccttgat gttgctgcac gcgggccatc attaatgtgg
     3781 catcttcgga acccgcaggc gcttcaacac gttcaatggc ctgattgacc cccgcgacct
     3841 gagccgcctg actttgcaac catgcgaccc attgcggcga aggagaactg gcggtagctg
     3901 cacccatcag acgagtttca acgccgacac catacatggt tgctgcgccc tgaatcgctt
     3961 gctgagcacg gtcaaaaaca tattgattaa tgacgtcgct ggccccgcgt gtttccactt
     4021 tcagcaacgc cgaggcagga acaacgttac gaccgcttcc tgcctgcata acgcccacgt
     4081 ttactctgga agctccttcg ctgtgcgggg cgattgcatg cagtgcaaga gtggcttgtg
     4141 ctgccgccaa caaggcattg taaccgtctt ctggttttgc gcctgcgtga gcggcggtac
     4201 cggtgaagtg cgcgtcaaat ttggtggttg ccataaaatt atcactgccg cacaccacgg
     4261 tgcccgcagg tacgccagtg ccaatgtgca cggcagtaaa ataatcaaca tcatctacga
     4321 cacctgcatc gaccatcgcc cgcgcgccac gcgtaccttc ctctgcaggc tgaaaaatca
     4381 gtttgatgac gccatgtagt ccggactcga attgtttaag ggtatgcgcc agcccaagcc
     4441 caatggtggt atgcccatca tgaccacagg catgcatcat tccggcgtta catgacgcaa
     4501 aaccgtcgcg gtaggggcga tggctgacat cctgctcttc actgagatcc agcgcgtcca
     4561 tatcgacacg gaaagccatc accggaccgg ggcgaccggt atccagggtg gcgacgatac
     4621 ccgtgaaacc accttcaaaa gccgcaatcc attgtgctag cgcaccctgt tgacgagcgc
     4681 gctcgaattc gcgttgtaga gtgagttcat caggtagtcc catccggcta ttttcattaa
     4741 ctacttcgcg gcccagcgcc agtgagtagc cgagctgctg caattcttcc gcaacaaggg
     4801 tggcagtgcg gaattccacc cagccagact ctgcatagtg atgaaaatca cgtcgccagt
     4861 gcgataattt tggggcaagc gaattaacaa attgattcaa agactccata acctttcccg
     4921 tcatcagtaa aaagtgtgac ccggttcacg tagcgatagt ttttacttat cactaactga
     4981 tttttcacag ttttaaccgt tcataaatta ccctgacaca atcatctgca ttaaagtaga
     5041 tgccagtttc tttggtctga taaataacgg ttatcggtgg cgtcatggct tttcaggtaa
     5101 aaattcatca aattcgggct tttgttgaag tggctcgtca gggcagcatt cgcggagcga
     5161 gccgaatgtt gaatatgtcg caaccggcac tgagtaaatc tattcaggag ctggaagaag
     5221 ggttagcggc gcaactcttt tttcgccgta gtaaaggcgt gacgttaact gatgccggtg
     5281 aaagttttta tcagcacgcc agtcttattc ttgaagaact gcgcgcagcc caagaggata
     5341 ttcgccaacg acaagggcaa ctggcagggc agattaatat cggcatgggg gccagtattt
     5401 cccgcagtct gatgccagct gtaatatctc gttttcatca gcagcatccg caggtaaaag
     5461 tacgcattat ggaagggcaa ctggtgtcga tgattaatga attgcgtcag ggagaattgg
     5521 atttcaccat caatacctat tatcagggac cgtacgacca cgaatttact tttgagaaat
     5581 tactggaaaa gcaattcgcg atcttttgcc gcccgggaca ccccgccatt ggtgcccgtt
     5641 cgatcaaaca gttactggat tacagctgga caatgccgac gccacacggc agctactaca
     5701 aacagttgag tgaattgctt gacgatcagg cgcaaacgcc acaggtcggt gtagtctgcg
     5761 agacgttctc agcctgtatc agtctggtgg caaaaagcga ttttctcagc atactgcctg
     5821 aagaaatggg ctgcgatccc ttgcacggac aggggctggt gatgttgccg gttagcgaaa
     5881 ttttaccgaa agcggcctat tatttgattc agcggcgtga tagtcgccag acaccactga
     5941 ccgcgtcatt aatcacgcaa ttccggcgag aatgcggcta tctgcaaagt taaaactgca
     6001 taaaaaaata gagtctgtcg acatccgcca gactctacag tacacacagc agtgcatccg
     6061 cgtcttaaat ccggcattgt ctcctctgcg ccggtgactg tgttataacg gttttaacct
     6121 ttagttgcca attttcttca gtgacagatt tcacgaaaat ataagttaca ttattaatat
     6181 cgtgaatgaa taatcatgca taagcatttt gcttaaaata tcggcaatat ttggaactta
     6241 ttactggaat tttgggtaat acgttgttgg accgacccgg tctggttatc atatcgcgct
     6301 cttaattgcg ggaggatgta acatgaacct tgacgacaaa tcgctgtttc ttgacgccat
     6361 ggaagatgtc cagccgctga aacgtgctac cgatgtccac tggcatccaa cgcgtaacca
     6421 acgtgcgccg cagcgtatcg acacgctgca acttgataat ttcctcacca ccggatttct
     6481 cgacatcatc ccgctaagtc agccgctgga gtttcggcgg gaagggttgc aacatggggt
     6541 gctggataag ctgcgcagtg gtaaatatcc gcaacaggcg agcctgaatc ttttgcgcca
     6601 gccggtggaa gagtgccgca aaatgatgtt cagtttcatt caacaagcca tggcggatgg
     6661 tttgcgtaac gtgctgatta ttcatggtaa agggcgggat gataaatcgc atgccaatat
     6721 tgtccgcagc tatgtggcgc gctggttgac cgaatttgat gatgtccagg catattgtac
     6781 cgcgctaccg catcatggcg gcagcggggc gtgttacgtc gcactacgta aaacggcaca
     6841 ggcaaagcaa gaaaactggg agcgccacgc taagcgcagt cgttgatctc gagacgcatc
     6901 cgtggcttat gccgccagca cgcggttgcg tccatcattt ttcgcccgat acaaagcatc
     6961 atcaacgcgt ttaaacagtt catcgatgct ttcatttcct tcgtgatgcg ccacaccaat
     7021 gctgacggta aagcgtggta agcccgaaat actcactttt gccacgctta cgcggatagt
     7081 ttcagccagc gaaagcgcgg tatccagtgg ggttcttggt agtaataaga caaactcttc
     7141 gcctccccaa cgaaacacca aatcgccttt gcgagcgcaa ctttcgagag tgcgggcgag
     7201 ggcgcataac acctcatcac ctttagaatg cccatagaga tcgttaatgt gtttaaaacg
     7261 atctgtgtcg atgagcaata agctgtaatc ctgagcgatg gcgaggtgct gcatttggcc
     7321 tggttccgta atgtgataaa actgtcgccg attcagtaat ccggtcatcg cgtcatggtg
     7381 agcagcatgt tccagctgct cctccagccg tttttgctca gtaatatcat gcacaataca
     7441 taacatgagc ttgtcgccat aaatttcaat cggtccggca taggtctgca catgacgagt
     7501 cgaaccatcc gccagcttat gaataaaatt cagaggttta tgaccaccgg gtaaatgcga
     7561 gatttcatgc atgataggca tgacgcgacg cccgagcata tttatttccc aggtatgttt
     7621 ctggcacatc gtttcatggt tataaccata gaaattgagc gcggcgaggt tagcatcgac
     7681 gatttgtcca tctcgtgacg ggtcaatcaa caacattggt gcagagttag tcagaaaaaa
     7741 gcgcgcataa aaaccttgtt ttttgcgctg ataatttgcc gagcgactgg cttttaaacc
     7801 cagcgttgcc ggcgcttcga taccttcgaa aataatcacc ggttctgttt ctgtcagctc
     7861 tcgcaaaaca agccgacagc ttaatgctgt ttcctcttct ttacgctgaa cagtgaggat
     7921 ttcgataata tcgtgttggt tttgcagatc ggagaggtat ttcggcagtt ctttttgtga
     7981 ggagacggaa tagggaccgg ttcgtagctg actaaacgtg aggtcttgca tcaacagtcg
     8041 cgccgcgctg ttggcataaa ttaactgttc ctcaaagggc gaaacgatcc agacaggact
     8101 ggtgagtaag tccagagtat tgaagttgtg cgtaatcatt gagatcccat tatttttatc
     8161 aatttttgtt gctatccgat cgcaaaaaag ccacgtcata tgatcagata attctgataa
     8221 tgatagacgc tatttaacac
//
LOCUS       DAZKFZ010000204         8092 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000204 DAZKFZ010000000
VERSION     DAZKFZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHOQW_003958"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHOQW_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP9574943.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="ACHOQW_003959"
     CDS             complement(399..1022)
                     /locus_tag="ACHOQW_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9574944.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="ACHOQW_003960"
     CDS             complement(1025..1306)
                     /locus_tag="ACHOQW_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9574945.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="ACHOQW_003961"
     CDS             complement(1299..1625)
                     /locus_tag="ACHOQW_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFP9574946.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..3737)
                     /locus_tag="ACHOQW_003962"
     CDS             complement(1713..3737)
                     /locus_tag="ACHOQW_003962"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9574947.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(3682..5184)
                     /locus_tag="ACHOQW_003963"
     CDS             complement(3682..5184)
                     /locus_tag="ACHOQW_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9574948.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGSKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(5184..5396)
                     /locus_tag="ACHOQW_003964"
     CDS             complement(5184..5396)
                     /locus_tag="ACHOQW_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574949.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(5393..7516)
                     /locus_tag="ACHOQW_003965"
     CDS             complement(5393..7516)
                     /locus_tag="ACHOQW_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9574950.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLWTERAEDNPWITSGGWL"
     gene            complement(7513..7989)
                     /locus_tag="ACHOQW_003966"
     CDS             complement(7513..7989)
                     /locus_tag="ACHOQW_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9574951.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         1752 a         2538 c         1924 g         1878 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccacg cggttcagtg tcgtggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgccc
     2821 atttcattca gcagtcgttc acgggccatt tccagcgaac agtcaggatc agccacgcac
     2881 tgtgcctgaa gcgtctgata gcgaccaccg aacatggcaa acagatcgtt aatgcctgac
     2941 atgcgggctt tctgttctgc cataacgcgg gcgcgaatgg tcgcctcatc agacactgcc
     3001 ggtaccggtg atggttctgt taccgccggt gcagggattg tcactgtggt atcacgcggg
     3061 gcactgttgc gtggcggagt aatcatgttt cggatggatt ccggcatctt tttaaattcc
     3121 tctgtacgtt ttgactgaat acatgccatt gcctcaacag cgggtgtcac ctggtcagca
     3181 aatccgtgtg ccagacattc ggcaccggac atccaggttt catccgccag catggcggca
     3241 atttcatcgg tggtttttcc ggttttctgc gcataggctg gcaacagtac cgattcgact
     3301 ttatccagca aatcggcata actgcgcata tcctcagcat ccccgccact gaatccccat
     3361 ggcttatgga tcatcatgaa ggcattttcc ggcataatga ccgtatcacc ggccatcgca
     3421 atcacagatg ccatcgaggc ggcaacgcca tccacataca cggtaatggt cgccccctga
     3481 tttttcaggg cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata
     3541 tggagattaa tgtgggtgat atcacccagt gcattcagtt cgctgacaaa ctgcttcgcg
     3601 gtaactcccc agaaaccaat ctcgtcataa atataaatat ccgcgtcacc cggcccccca
     3661 gcctgcatcc tgaaccagga tttattcttc atgctggctt tcggtgtcgc gctgatactg
     3721 tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg gggtcagtat caaagaccag
     3781 ccccagtctg ctgttttcat caatttcagc cttgcggcga cgtttgacct catccggatt
     3841 gcgcccacca gcacgcaccc agtcagattc tgtcgctgca ccaccccgga tctgaattct
     3901 ccaggcttca gcttccttaa ccgggtcgat ccacggcatc accggaccgg aatacgtcgc
     3961 gttatatagc gttttcatct ccacatccgc cggaattttc agcagacctg ccgcaaccac
     4021 catattcagc catgtccggt acaccgggcg ggttaccgcg ccaataaaac agtcctgcag
     4081 gatcaggtaa ccatccgtgg actcgaccaa ctcctgccgc tgggcgctgt aggtgccgtt
     4141 atagttacgc gccgcactgg aaaaactcag acgactgcct gctgccactg cacgcaactg
     4201 gccgttgcgg aaagtttcaa ggttgggatt gggacggtca gatttgacca tgccgatatc
     4261 ctcgcccttg cgcaaatcgt cataaataat acccggggtg atatggactt cccgctcggt
     4321 ctctttgctc cccggatctt catagtcctg tccgtcacct ttacggatat acagtcccag
     4381 cgccgcagca atacgcgccg ctgtcagttc cgcatcctca tactccttaa gggcactgat
     4441 ccgcatcagc acccccgata acatggatga gcctcgcgtc tgatgcagac gacgagtgaa
     4501 cttcaggtgg atcatttttc cggcagcgat ttctttcgta tcactctgcc ggccgctgac
     4561 cggataattt ttataaacca gatatttttt cggtcttccc cactcatcaa gaaaaacccc
     4621 ctgattcagt ccggcggatt catcagtgcg catgggaaca aaatccggct ccatcgcctc
     4681 aagccagaat ggcactcccg ccgtccgttc cagaccgttt cccgcaccac tgaccatctg
     4741 cgcaaacact tcaccatccc gcagccaggt ccgcagcagt aaacgttcaa gcacaggacg
     4801 ggtatactgc cctgtcacat ccggactcac ggaccattca gcccacaaac ggcggatatc
     4861 cgcagccagc tcagccgcca tttccccgtt ttttcgtaat ggctgaggct ccacaataat
     4921 tcccctggca ccaatcaccc gctcttccag cttgtcaaac acaccaatca ccaggtcatg
     4981 attgatatcc agaaaacggg cctgctcccg cagggaaacc gcaccgtatt tactgagctg
     5041 atcagcagag cgattttccc gccgggcttt atgtgtccgg gtcggtttca ccgcctcata
     5101 ggccatgatt aacgcccttg aacgcagtct ggctgctttc cacccggggg aaaacacgcc
     5161 gatcacatca tcaataattg ccattaaaac ctcgccagtt taaatcccgg ttttccccgc
     5221 ctgcggctca ccatcgcggc aagcctgcgt tcccactcct gacgtccggc gcggatctga
     5281 gaaaggcttt ccagcgtcag ttgctgcccg ttgaaggtga cagactttcc ctccagtacg
     5341 gccatttccg cttcacggta ccgctgtatc atttctctgg cttcttctgt gctcacaacc
     5401 agcctcctga tgttatccat ggattatctt ccgcacgctc cgtccacagt tttttcttcc
     5461 ggcgacggcg tttttctgcc ccggccgtca gttccgggga taccgtttca ccagaacgct
     5521 cctgcgggaa gacgagccac gtttcccgct gtgcccagtc cggtgcggag ggccagcgga
     5581 tcttttcgta accatgcaga acggcaagcg catccgcata aaccagcagg tcaaacgctt
     5641 cgttagcgcc cctgcccggt tttcgccatt ttccgtcact gccgcgctct tcataggtca
     5701 gctcatcgta aaaccaccgc cccagccagt cgggaaagtg gatatagttc ggccctggtg
     5761 tgtcacgcca cagggcatta tttacacgat ccttaaacgc atccgtctga accagccaca
     5821 gcgcgacatc gccactggct ctggcacggc gggcacttct gccggtatta tccgggaagg
     5881 ttcggttaat cagcctgtca cggcgaagtc catccccctt gaacagaaac accctgttgc
     5941 ccagtccgtc actccggcaa cgacgccaga aacgataggc gttatctgtc accccggctt
     6001 cccctcccgt atccaccgcc atggccatca gacgcatgcg cacatccgga tcagaagcca
     6061 gcggccatgt tttatggaac acatccgtca gcaacaaatc ccagtcctcc ggatatgccg
     6121 ccggatcaac cggcagactt tcaccgttgg gactgcagcg cagtgaatgc cggatgttgt
     6181 agcgatcaac aatccagcgt tccccctgct ctccgtatcc ggtgatctgc acaacaaaac
     6241 ggcgattttt accgccctgt acgtcaaccg ttgcctcaat aaaacgcaca ccatccggca
     6301 cagatcgccg gggaaacggc tcggcacgct gttcaagcag ttcactttta cgctgttccg
     6361 tggctgaacg gggcagatag ggtcgtccga tatcggtgtt caccaccgct ttcagggtct
     6421 cttcactgcc ggttcgctca tactcttctt ctgccgccag cagtttaaaa atcagttgtt
     6481 cccaggtctg aaacgccgca gctggcccct ccatccaaaa tgacgcaatc cgggagtttc
     6541 gtggcgttcc ggtgatactg ccgtccgccg ccgcccgttc accttcacga agccagatcc
     6601 cctggttatt cagttcgcgt ttctgctcag gggcaatcag cccgcgacaa tgcggacaca
     6661 tcagacgggc agcctgaccg gcagccacaa aatccgggtt attccggtat ccggtcatgt
     6721 tatccatcac cggctgaaaa tattccccgc agtgcggaca cggccagtac caccggcggc
     6781 ggtctccccg gttatacagt gacaggatcc ccgttgttgg cggtgcctca tgtgcgccac
     6841 cacaacgcca tttggtatcg gtgatatccc gccccggtga actctcgacc agggtcatcc
     6901 ccgaggacat aaaggtggtg gtacgctttg aggccagcgt gaaggcatcc ccttccccgt
     6961 ccacgttttc agggaaacgg tcataatccg tcagcgccac acgacggtaa tccgaagagg
     7021 aaaagacggt gatcgacggc cagccaatct tcaggaagga gccgtcaaga aacattttat
     7081 cgtggacgtt gttgtcatta cgggaaggac tgaggcgctt gctgacctcc ggactgtggc
     7141 gaaacgtcct ggaaagacgc gttctggaat gctcacgcgc cttcgtctca gtcatctgca
     7201 ccaccagcat atccgccgga tcacagatga tgccgtacac aatccagcca tcaatcagcc
     7261 cttcggtttt cccggttcgc gcaggtccca caaacaccac cgcgtcatat tcacgggctg
     7321 ataatgtatt aatggggtca atcatatagg gcgtcagcga tgactcccac ggaccggaag
     7381 tattggctcc ccgtggaacc cgcatataac gcctgatggc ttccgctact ggtaaccggc
     7441 tgggtgggcg aaacagcgag gccacttcgc gccagatatc ggatgcgcgg ctatggctct
     7501 cgttcacctg attcacatat cggcctcatc acaacagtca atgactgcct tttccagtgt
     7561 gtcgcggatc tcatcaacca caatctgtac ttcattcagt tgtgatgcgg tccaccccct
     7621 gtccctctcc agccggtcag gccaggtttc cagtacctga actatcgctt tcaccacgac
     7681 agaaaaggac cgcctgacat cactgactgg cacaagctga ccagtttcct gctcaaattt
     7741 cagtcggtca cgttctgact ggtaccatgc tttacgcgca tgaggatcca tttcctcgtt
     7801 atctacaggc agaggagctt tcatcagctc ggcaaggata tctgtcagtc ggtacagttt
     7861 gagattgctc tcatgaccac cggctgggct tatgtttttt acccgagccg caacagtctg
     7921 tcgatgagca ccggataatg cggccagttg ggaaatattc agatgcagat tttttaattc
     7981 acgatccata actccccctg aaaattatgt aaacacacac cagtgatgaa caaaaaaaca
     8041 accagattcg acactaaaaa tttttatttt tctatatatc aacaacttac ac
//
LOCUS       DAZKFZ010000205         8091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000205 DAZKFZ010000000
VERSION     DAZKFZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(64..251)
                     /locus_tag="ACHOQW_003967"
                     /pseudo
     CDS             complement(64..251)
                     /locus_tag="ACHOQW_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(371..1153)
                     /locus_tag="ACHOQW_003968"
                     /pseudo
     CDS             complement(371..1153)
                     /locus_tag="ACHOQW_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1869..2327)
                     /locus_tag="ACHOQW_003969"
     CDS             complement(1869..2327)
                     /locus_tag="ACHOQW_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9574952.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            2450..3223
                     /locus_tag="ACHOQW_003970"
     CDS             2450..3223
                     /locus_tag="ACHOQW_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574953.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIGSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            3375..4691
                     /locus_tag="ACHOQW_003971"
     CDS             3375..4691
                     /locus_tag="ACHOQW_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574954.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(4890..5459)
                     /locus_tag="ACHOQW_003972"
     CDS             complement(4890..5459)
                     /locus_tag="ACHOQW_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9574955.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            5652..6119
                     /locus_tag="ACHOQW_003973"
     CDS             5652..6119
                     /locus_tag="ACHOQW_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFP9574956.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            7123..7455
                     /locus_tag="ACHOQW_003974"
     CDS             7123..7455
                     /locus_tag="ACHOQW_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFP9574957.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            7577..7954
                     /locus_tag="ACHOQW_003975"
     CDS             7577..7954
                     /locus_tag="ACHOQW_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HFP9574958.1"
                     /translation="MNLDEEFYELQKLFAQKDLLTEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
BASE COUNT         2353 a         1852 c         1792 g         2094 t
ORIGIN      
        1 acccgtttta gttccataca tctttaaaaa tcccgaccaa ataatgagat cagcaaacat
       61 ctatttgaaa cgttggttct catcctcaag atccttgatt attttaatat cagaaaattc
      121 cataccgccg tatctggact tgcagttgta gtaagtggct tcagataagc cacatcttta
      181 acagttcgcc cgtattcaag cgatttaatc acggcgataa tctgatgctc agtaaaaggg
      241 gctttacgaa tagcgatctc cttcattggc agattgatta tgccggagga tctctaaatg
      301 tgaatggcac gattatgcgg gatatttact gtaccagtcc aaccctctgc ttactgcaat
      361 accaatcaag agggatggtc cacattgacg tttcaaaatc gatttctgac cagcgagcaa
      421 aatgcagctt actggaccga ataaagataa gcagagtgag ttctgtcgta cagtgggtta
      481 gcggtctgcc ggtgtagctg tctattttgt caagcaactc agggatacgc tttaactcca
      541 gcgccggacg atgctgtcga ttactggaag caaccgcccc cgacatctct tgcgccggat
      601 cgtaatcaat taacccgctt tgcactgcat aacgcatgat ggccgtggtg cgctgttgaa
      661 ggcgggaagc cacttcaaga cgcccggatt tctccacggc tttaatagga attaacagat
      721 cacgggtgcc tagttcagca atgttgcgag taccaattgt cgggaagata ttgtcctcca
      781 ggctcttttt tacacgatta gcatgatctt ccgaccattt ttggttggtt acgagccact
      841 ctctggcaac cttctcgaaa gtaataatct ctttcgcctg ctcttctttc acagcacgct
      901 tatgctcacg aggatcgata ccagcagcta ccagctttct ggcctcttcc cttttcttcc
      961 gcgcatccgc cagtgaaaca tccgggtaaa cgccgaacgc catcaaatgt tgtttaccgc
     1021 caaaacggaa acgaaatcgc caatactttg aaccgccagg ttttacaagt aaggacatgc
     1081 cgtcgccgtc aacaagtgaa tactctttct cctcaggttt cgcagagcga actttagtat
     1141 ccgttagggc cataatggtc ttccttccat tggtatctcg tgagtataca agcaatatcg
     1201 aatcggcaaa tatactcatt agtatactca caagcctgtt gatgtgggtt ggttcaggtt
     1261 gacttcagtt gaggagaaaa gctggaaaag ccttgcgtgg cgcggatttc aggcataaaa
     1321 aaagacctca gttgaggtct atttacatac ttttggtgcc gaaggccgga ctcgaaccta
     1381 agtctccata acctgctgta atcacgttcc tattagcctt taaaagaaca atcgtaggac
     1441 ctttttgttc taggggctac atcttaagtt tgatccgctg gacggaatca aaccgagtgt
     1501 tcgtggcggc acaaatcatt aacgtgccat acctgccttt tgatgtttat ggaggtctag
     1561 tgggtcggga ggaaagaaca tttgtcagct atgtggatgt taagtaaacg ccactgcgtt
     1621 cggattttga aaccagttaa accccgtcag accttgttca gaataactat ttaactcaat
     1681 gttatttata tgaattaagc cattcagcgt caaagtaaaa cgccaccgta acgcctacgt
     1741 aacgcacccg cattttgctg ttttattcac tcttatagaa acaaagagct gatgaaacgt
     1801 gaggctatca gcatctaatg aacactgttc atccatacag catgaaaatt cataaactta
     1861 cacttgagct actttctcag cttgtcccac cgaaccgcag atacaatacc ccagttgccg
     1921 ctaggattag gcctacgcca ttttgctgtc tccatgctac gaagatactc aagagcttca
     1981 gaaaacgtgc caaaccgctt ctgagccgtt ttatcaccta cgccaaacga gccatcgcga
     2041 gtgcttgcca gcccgggatg aaaacacgag ccatcagctg cataaggaac cagtaattcc
     2101 gggtcactat cttcagatga atcagacacc aatagtcgcc acggctcaac actcagagca
     2161 ttagccagca cctcaatcgc atccaaagac gcattagagc caccgcgttc aatgcggctc
     2221 atatagctac gagcaaaacc acaccgatcg gcaaaggctt cttggctcat tccggtggca
     2281 atgcgcaact cttttactcg ttgtccaaac agttttctca gggtcatttt catgacctga
     2341 atttgcgcag atgtccacta aagagccacg ctccaatagt gacattttga atcaatggtc
     2401 tatgatgcga taactgacac cgctggggtg gattctgagg gataacaaca tgtcaaaaac
     2461 acggcttagt aagcccggtt tttcagccaa gtctgtgctg accattatgt taatcgctgc
     2521 gtcatctgca tcttatgcag cagaaagcat cgatcaactg gcgacgcgca tcggtatgga
     2581 tcacttccgg gaagcgatca aaaagggtga acgcagcgcc ttcaaaacgc tgccggcaaa
     2641 aggctataaa aacggtctta ccgggctgtt aatcaaaact aacacttacg ttaaccccga
     2701 atacagcttt gctgattgcg atacggacac cgttattatc tcggtgcaag aacacaaaac
     2761 gcttcccggt tgccgtatat ctttaaagct agcgacaaaa gacgcagatg gcagttatca
     2821 cgacaatgga attcgcgttg aatacggtat tgccatcggc tccaaaaagt ttatcgctaa
     2881 caattcagcg tgggcgcaac atgccatcag cggtgataaa gtgttggaag gcgctctcca
     2941 cccggataaa acgaataaca ataaagatca aatgaataac aataaagatc aaatgtgcat
     3001 gggtatggct caatttaccg caaacgtaac cgaaggccgt aacagtggga tgacgaaaag
     3061 agaatattat cagcgacttg ataacatgaa cggtaaggcc gacgtcaccc agcagatgat
     3121 aacgctctat aagacttttg ttgactacgt atatgaacat ccaaagcaag acgcaaagat
     3181 ttcagggacg tatatttata ctaactgtat gaacaacttc tagaaacgta ggtgcctgaa
     3241 catgatagaa ctcagctgcc agcatagagt ggaaagcgga ggcttaccat cgtatattca
     3301 tcgcgttatc tttccgtaac tataatctgt ctcagttgat ctagtgcctt tcagggctga
     3361 actaaggaat aaagatgaat gacggtgtta tgctagattc tgacgttatt gctggtcttg
     3421 agtggttagc atcaactcaa gagaacaaac aacactttta ccagcgctta gcaaaagcac
     3481 agcaatttta catagctaca acccaaaaag ctgccaattt cgggaaacag tttgacccag
     3541 aatggtatgg ttccgacgtg gttgctggtt atttcgcaca agcaaaatcg cttatcgata
     3601 atagaaggtc ttacgaagtt actaatgctt caaatataat accatgggtt aaacaattag
     3661 gagtgtgtgc taaatcgctc gatagaatta ctggcgctag agatcgcgca atcagaatgc
     3721 ttaagaatac aacagtatta ccagacacag ccttattaga gttggttctt gctggaaact
     3781 atgcatcaga aggttatgaa gttgagttca ttccagaaca aaaagggata gcaaaaaccc
     3841 cggagtttcg ttgccgcctt ggagatggcg attatttctt tgtcgaatgc aaaaggcttc
     3901 agaaaggtcc ttacgtaaag caggaaatat tacagcatca tatacgctca catcttgccg
     3961 aacttcatat caaatccaaa aagatgaacg tatggactga tgtcacatat ttatgtgaag
     4021 ttaaagacgc gcctgaaaac tatatcatta gccacttaaa aaactttaaa ggtgtatttt
     4081 atgaatggaa tgatgattat ggaaaaggaa cgattagacc ggccaactta aatcttatca
     4141 gggaagatat cactgaaaac ggctcattac tagtcaacac taaacttgct cgtcttatca
     4201 aaggctcccc tctggaagat gaaaattacc aagttgttgc tatgggacga ccagatgagc
     4261 gcgactcacg tttcataacc aaagttaaac ttgcttcttt gataacatgg cgttgtatca
     4321 atgaaaaatc tttcgatgca cgttcgcgac atgtaacaaa aacattagca gagatagata
     4381 accagctttt ggattatggt ctaggcgtag ggcatatagc tttggatgta gatattcaaa
     4441 aggatgttgc cgataaacga agggaaaaaa actatgctgc catagtaaat tttgagcaaa
     4501 aatctaaaat agtccgcctg aatgttcatt atctcgtgcc acgcatagat gagaactctg
     4561 cgtggatggt agatgaaact gcggatgact tttttaccca tgatctggtc aaatatgtta
     4621 tcccattgac taaaatattc cccgaggctg aggtatttga gaatgaccaa cctggctggc
     4681 atcaaaatta atttattttt gatagtatga acgagggtag catttctata caaatcagtg
     4741 ttcggctgat agcgagttaa acagtttact gaaatgttct cagcaattag ctgttgccag
     4801 gacctcaaaa atccgattct ggcggaaagt gtgctgtttt accaagtatt gctcgtatct
     4861 gcaaagtgtt agagcatgtt ttaagtatat cagtaaccct tattcagtaa tgcagcaagc
     4921 gtcgctcttg aaacaccgta ttcacgcgcc aatgcgctaa cactaacccc agcggccctt
     4981 ttgtctaaaa tcgctccacg agcttccggt gcgattttag caggcctgcc caatttcctg
     5041 ccttcagctt ttgcccgtaa cagccccgca ttagtccgtt caatcagcag gtcacgctcc
     5101 atctccgcaa ccgctgctag catcgacaac aggagcttac cggcagcaga ggtgagatca
     5161 gtggtgccaa gctgatggac aataaccttg atgtttcggt cagccaacgt cctgacagtt
     5221 tgcagaacgt caattgcatc acgcccgagg cggtccagct ttgcaactac caacgtttcc
     5281 ccatcgcgga ttttgctgag catttcaaaa aacgctttgc gttgcaccgc aggaaccttt
     5341 ccgctgacta catcagaaaa ccaaaagtcg aacgtccacc cagcttgttc aagttccatg
     5401 cgctggttct cagtatcctg ctgggatgtc gaaacacgcc cgtagccaaa aatagccata
     5461 gtcatctcct catccggccc ctgccagaaa acggtgatga aaataggatg gcctgccgga
     5521 aaagccaagg ctaattttcc aacagtgtga aagcgtgcgg ttggaggatg ccggaaaacg
     5581 gtcgtttgtt ggcgcttaaa acgggtgaag acatgttagg ctgaaaccat cacttcataa
     5641 ggacaaccga aatgattaaa gacgcgattt tttcaccgtg cggtaagtat cgctactcgt
     5701 tatcacgagt ctgggacgaa tcaaaaccgt ataccctttt catagggcta aacccttcat
     5761 acgccgatgc cgagaaggat gatcgcacct tgagccgctg catatcgttt gccaaaagct
     5821 ggggctacgg tggcgtatac atggccaacc tgttcgcatt cgttcacacc cagcgacatg
     5881 aaatgatgaa agcgtctgat cccattggca aagataacga tagccacttg atacgtcttg
     5941 ttagcggtgc cggactggtt gtcgccgcat ggggaaatga aggaagacac ctcaaacgct
     6001 caacgaccgt ccgacagcta ttgccagaga gcacaatgtg cttcgtctta aacgctacag
     6061 gtgagccgaa gcaccctttg tatatgaaaa acgacagtgt tctcatacct ttaggttaac
     6121 atcgataact gcgttcagaa tatgaacgca gccatatgat atatatgact tttctttaaa
     6181 atgatggtgt gctagcttct ctcctgacac ctacctcatg cggtaaaccg gtccgggtag
     6241 gtggagatca ccgctgttta gcggaagacg tcaggatggg aactctgcgt aacgttgatt
     6301 gtggtaggta gggcgcgcaa gcgtttgatg cagccaagca gagggtgaaa gtagctatcg
     6361 gcggattctt aagtagcttc ttttgcgcat aatgcatctt tcaagaaagg tcgtcacacc
     6421 acgaggaaga cttgatcttc atctggcaag gggaaccgag caaacctaca ggctatcttc
     6481 tcagatggcc ttagggttga tatgtccggc ttcccccccg aggaagggtt tacatcttaa
     6541 agaacaagtt atgttttaac atttttaaca attacacttc tctgctgttt ggtgtcttat
     6601 ggcgttcccg tcaaaagtaa agctgccgtt tttacctcac atgcgtaaca accaaacacg
     6661 aaccagaagc tgctaacgtt caacgtgttc gcggtgtaaa tgcagccgga taagttggga
     6721 gcgaaaaagt ggatgtccta ggtttggaag cgcccctctt aagcattgca tccgataatt
     6781 tccgtggcgg tgagacagtc cttatagagg caatcagcct ccaaagttaa gcgacgcggt
     6841 aaaactaaag ctcgcccaga tttctcattg attgctcgaa ctccgctacg cttgtttcaa
     6901 ctggcttcac cagtcactct atgagagcgc cgcgaattct gcaccatgat ttcgactacc
     6961 ttctttaaaa cactcgccag agagccttac agcgcgtttg aaccgcatat cgcagcttct
     7021 gcttacggat gactgtctga ttagcagtag cttcatggcc cagtccggtt tactttattg
     7081 ctatcaacta ggagccgccc aagggcaaca cggagataac gcttgagaac cgtcgaaata
     7141 ctgcaataca ccttacgcaa aggcagcggt gccgcttttc acgccataat gcaggaaatc
     7201 agtgttcccc tacaccaaag ccacgggatc gatgttgttt cgtttggcaa ctcgcttgat
     7261 gacctggatt gctactactt gattcgcgcc tttgacagcg ctaaaagcat gactgcggtt
     7321 ctcgatgctt tttacgcaag cgcagactgg cgcagcggcc cacgcgaaga catcattggc
     7381 agcatagaga actgcattaa aacagtgatt agcctaccat cagaaagcgt gaaagggctg
     7441 cggatgcaat cgtaagtaat caagtgtatt agcttagatt tgacctaaac agctttgtgc
     7501 caaaagtgga ctaggaaaag cctagcgata aagagttgag tttaataact actaactgaa
     7561 atttaatggg tgatatatga atttagatga agaattttac gaattacaaa aactctttgc
     7621 tcagaaggat ctactgactg agaaaagacg ctctagtggc ggcggattca tggaaattct
     7681 cttggtgaag agacagaaca tgaagataaa aatatatcaa gaaaaaggac atcaacttcc
     7741 ccatattcat attgactatg gaaaacaagc gcatgctgcc agttatgcca tacaatcagg
     7801 agaccgcata gagggagatt tacctaagaa atatgatagt gatgtttcga gttggttggg
     7861 gagaaatcgt gataaaattt tagaaatctg gaatgcacta caagctggcg caccttatga
     7921 accactaatc gcagaattga ctggcggagt gtgactaact aaataatttt cccctaaaaa
     7981 aattcgatgt aggaaaatat atataagtaa gaatcatgag tgtccgctcc tcgctcaagg
     8041 cggactgtta gacttgattg tatgctgtca gagaaaaccg tcagatcaat t
//
LOCUS       DAZKFZ010000206         7977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000206 DAZKFZ010000000
VERSION     DAZKFZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.206"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(178..1035)
                     /gene="murI"
                     /locus_tag="ACHOQW_003976"
     CDS             complement(178..1035)
                     /gene="murI"
                     /locus_tag="ACHOQW_003976"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9574959.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(980..2824)
                     /gene="btuB"
                     /locus_tag="ACHOQW_003977"
     CDS             complement(980..2824)
                     /gene="btuB"
                     /locus_tag="ACHOQW_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP9574960.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2889..3079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3193..4293
                     /gene="trmA"
                     /locus_tag="ACHOQW_003978"
     CDS             3193..4293
                     /gene="trmA"
                     /locus_tag="ACHOQW_003978"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP9574961.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4333..4692)
                     /gene="yijD"
                     /locus_tag="ACHOQW_003979"
     CDS             complement(4333..4692)
                     /gene="yijD"
                     /locus_tag="ACHOQW_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP9574962.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4692..5342)
                     /gene="fabR"
                     /locus_tag="ACHOQW_003980"
     CDS             complement(4692..5342)
                     /gene="fabR"
                     /locus_tag="ACHOQW_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP9574963.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5446..6762)
                     /locus_tag="ACHOQW_003981"
     CDS             complement(5446..6762)
                     /locus_tag="ACHOQW_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFP9574964.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6805..7971)
                     /locus_tag="ACHOQW_003982"
     CDS             complement(6805..7971)
                     /locus_tag="ACHOQW_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFP9574965.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2084 a         2172 c         1896 g         1825 t
ORIGIN      
        1 gctacagagt caagcattta tttttgcttt ctctgtcgga attctcagga gaaccccgct
       61 gacccggcgg cgtgtttgcc gttgttccgt gtcagtggtg gcgcattata gggagttatt
      121 ccggcctgac aagaggaaat ttaaaataat tttccgaccg cgcaacattc aaccaaatca
      181 gcctaaaact gccagttttt cgagcgtttc gaagccgtaa cgctgtaaaa cgggcaataa
      241 ttgttcagct tctggcgtca ttgccataca aaaggcaata ttcgcatcgg cagattttgc
      301 atccggggct tcatgttcta acaaccaggc cgttcggcga gcaatcgctg cgccagaatc
      361 caccagccgg gttccctctg gcagcacttg taacagttct tcttgtagta gagggaaatg
      421 ggtgcaaccc aatacaacgg tatctggcgg ctctttcatt cttaaccacg ggcgtaggat
      481 acgttttagt gcatccagag aaacatcttc gccatgcagc ttcgcttcag ccaactcaac
      541 catctctgcc gagcccagca tttctatctg gcattcatta gcgaaacgcg cgatcagctc
      601 atgagtataa gaacgtttaa ctgttccgcg ggttgccagt aatccgacaa tgccatttgc
      661 cgtcagacgt gcagcaggtt taatcgccgg cacgacacca acaaccggga agtcgaactt
      721 ttcgcgtaat gcaggaagtg aaacggtact ggcagtgttg caagcgacca cagccagcgc
      781 aaggggataa cgttcttgca ccgcggtgac aattgccacc actcgctcaa caataaacgc
      841 ttcgcttttt tcgccatacg ggaaagcgac gttatcgaaa gcataaatgt aatggagatc
      901 cggtaagaga tgccggatct cgtcatagac cgacaaccca ccgacgccgg agtcaaacac
      961 cagcacggtg ggacgtggtt cagaaggtgt agctgccaga caaggtgtat tcccgtcctg
     1021 cagtttggta gccatagact gtctcataat ctttgtcgaa caggttggct attttaccac
     1081 gaactgtcag gtgagaggtg accggatacg caaccgcaag atcccacaag ctcacaccgc
     1141 ccattttaac ggtttgataa ggataagatg agtaatcctt atcatagcga gtgcctaaat
     1201 actgataagt aataccccag tcgaagtcat acaactgcca gtcgagctgg tatttcacct
     1261 gctgtttagc acggcgtaac aacggcgtgt cggtaattgc attgcgcgca tcgacataat
     1321 cataactcac agtatgcgtc agtggtccgg tatcaaaatt ggcggtcgcc tcgacgccct
     1381 taatccgcgc tttcccttcg ttgtaatatt tcagggtgtg atcatcataa tcgatcaagt
     1441 cactgacatc gttacgatat ccggaaatac gccagttcac cccagcggtt aagccttcaa
     1501 acgcgccttc ccactgtttg cttttctccg ggtccagatt cggatttccg tagaagccat
     1561 acagttgccc cagatttggt gccttataag atgtcccgta ggaagcaatg aagcgataac
     1621 cttcgatgaa ttcccaaccg gcgctggttt gccaggttcc atgacgacca aactgtgagt
     1681 tatcgtcgct gcgtgctgcg ccttcaaagg taaaatcgcc gacttgttgc agcccggtca
     1741 gatagatgcc ggtattacgt tgatcatatc catccttaac ataagctgtg cccggtgccg
     1801 tgctctgctt ctgccagtca acacccgcac caacattacc gtggccaatg atgatgttgt
     1861 ttgcccactg gacggtgtat tgcttcatct catcgagcgt cgccgacgaa tcataacgac
     1921 cataatgggg atcgtagttg taatctttgc tatggctata gctggtaatg agttgtgatt
     1981 taatcagttc gccgttatat cgcagcccgg cgtcccaact ttgactatag agtttacggg
     2041 tatcgaccaa tggtgaaccc ggagaataat acgcgtcata attggtacgg ttatcatagc
     2101 catagccgcg cacaaagccg ctccaggcat cagtaaagtt atgctccagc gcgccataaa
     2161 gcgttttact taaaaaacca tcgttatctg gctgcgcttg cgttccggta ttaccatagg
     2221 caacaacatc ataaccatga gtatgggcat aatcgcccaa caacgttact cgtgtcttat
     2281 cccccagttg ttgctgtgta gagacatcat agttttgata actattgctt ccccaccctg
     2341 ctgaaatttc cgttccgggt tcatcgcgcg tcgtgatgat attcaccacc ccacctattg
     2401 catcggaacc ataaacggcg gagcgtggcc cacggatata ttcaacacgc tggacaagcg
     2461 caatagggaa ctggctaagg tcggcagaac cactcccccc cgccagattc aggcgtacgc
     2521 catcaattaa caccaacaca tgactggcat ttgtaccgcg aataaaaata gatgagagct
     2581 gacctgaacc gccgttttgg gtgatatcga cgcccggaag acggcgcagc acatcattaa
     2641 ccgaggtcga ctgccagcgg tcgatatcct gacgcgtcac aacggtggtt ggtgcaagca
     2701 cagtgctgcg cggctgttca aaacggttag cagtaacgac gagagtatcc gggctggtat
     2761 cctgtgccca agcggaaaag gctgtgacag aacacgccgt cagcagcgaa gcttttttaa
     2821 tcattgtaaa gcatccacaa tagaagaagg atgccgcagg tttcatcaat attacgcgat
     2881 gatgagaacc agatgcgacg ttggccggca ggtcttcggg cttggagggg tatctaagat
     2941 actaagagat gatgacttcc caccgaatgg cagtgtccgc ataacgcaat catcgcacct
     3001 ttccttaccg ctgcgcgtca gctccagatt cgcactggat tccctattaa ctcacaggac
     3061 cggcaagtgg atgctacagg ttgtaacaag ttactgtcca gacgtagctc acaaatagga
     3121 attcatcaag atctggacat ctgatgagca atccctacaa tcgccgcgta ctttaatttt
     3181 tcaggataca tcatgacccc cgaacacctt ccaacagaac agtatgaagc gcagttagcc
     3241 gaaaaagtgg tacgtttgca aagtatgatg gcaccgtttt ctgacctggt tccggaagtg
     3301 tttcgctcgc cggtcagtca ttaccggatg cgtgcggagt tccgcatctg gcacgatggc
     3361 gatgacctgt accacatcat tttcgatcaa caaaccaaaa gccgtatccg ggtggatagc
     3421 ttccccgccg ccagtgaact catcaaccag ttgatgacgg cgatgattgc gggtgtgcgt
     3481 aataatcccg ttctgcgcca caagttgttc cagattgatt acctcaccac gctgagtaat
     3541 caggcggtgg tttccctgct gtaccataaa aagctggatg atgagtggcg tcaggaagcg
     3601 gaagccctgc gcgatgcact gcgcgcgcag aatctgaatg tgcatctgat tggtcgggca
     3661 acgaaaacca aaatcgcgct ggatcaagat tacatcgacg agcgtctgcc ggtcgcaggg
     3721 aaagagatga tctatcgtca ggtagaaaac agctttaccc agccgaacgc ggcgatgaat
     3781 attcagatgc tggaatgggc gctggacgta accaaaggct caaaaggcga tttactggag
     3841 ctgtactgcg gcaacggtaa cttctcatta gcgctggcgc gcaattttga tcgggtatta
     3901 gccaccgaaa tcgccaagcc atcagtcgct gccgcgcaat acaacattgc cgctaaccat
     3961 attgataacg tacaaattat tcgtatggcg gcagaagaat ttactcaggc gatgaatggc
     4021 gtgcgcgagt ttaaccgcct gcaagggatc gacttaaaga gttatcagtg cgaaaccatt
     4081 tttgtcgacc cacctcgcag cggtctggac agtgaaaccg agaaaatggt gcaggcgtat
     4141 ccacgtattc tgtatatctc ctgcaatccg gaaacgttat gcaaaaatct ggaaacatta
     4201 agccagacgc acaaggtcga acgtctggct ctgtttgatc agttccccta cacgcaccat
     4261 atggagtgcg gcgtattact gaccgcgaag taaaacccat gccggatgcg ccagcatccg
     4321 gcatgatacc gattactcag caacttcctg cttacggtta cgcatcttcg cgccaatcca
     4381 gaacaccata atgacggaga gtactgccgg gaagaagtta gagccgatat ccggatattc
     4441 cgcacgaact accgtgctgt acagcagtac gccgagaata aaacaggcag ctgccagacc
     4501 cggcaagcct accggcatgg tgcgattaag ataacgttga tgcaggcagt aaaccgtcag
     4561 caccagggaa ataatcggga atacagaaaa tggcacaatg gagctaaaca gcgctgcgaa
     4621 agtaccatta atcgataagc cagcaaccaa cgccagcagc agcgtacctc tatcttgatt
     4681 tgcttgtttc attactcgtc cttcacattt cccggaataa ttgcggtttt ctcttgttca
     4741 cggcgatacc agtaataagc ccctttcgaa atcattcgca gttgcagtac cagtcgctct
     4801 tctaattgcc gacgttgttc aacgccgacg tccaacgcct cggcacccgc actgaagaca
     4861 attgtcacca ttgcttcggc ttgcgcttca gtaaacgcac gcggcatatg gttttcgagt
     4921 tccagatagt ccgcaagttc cgcaatgaag tgctgaattt cacgcgcaac ggcggcacga
     4981 aacgcagcgg aggtgccgga gcgttcccgc aataataacc ggaaggcgtt aggattatta
     5041 ccgatgaact ccataaatgt ggagaccgag gtgcggatca cactcccgcc tttggcgata
     5101 cgctgacgcg cctggcgcat gagttggcgt agcattaaac cgctctcatc aaccatggtc
     5161 agacccagtt cgtctacgtc tcggaaatgc cgataaaaag aggtgggagc aatgcccgct
     5221 tcacgcgcca cttcacgcaa actcaggctg gcgaagctgc gttcagcact taattggcta
     5281 aatgcggctt ccaccagcga acggcgggtt ttttcttttt gttgcgctct tacgcccatc
     5341 acgatgtctg aatccttgcc aaaagtactg ctggcactat accagagaat gaacatactg
     5401 gattgctcca gtattgtgaa tgaacggtaa cgcaataaaa catatttact gcaattcttt
     5461 ggccggttct tttacgtaca acaacgccaa catcgacagc aacgtgcctc cacccagata
     5521 ccacgccgga gccgtcaaac tatccagcaa cacgatggat tgcgtagcga aatattgagc
     5581 aaaaccgcca aagattgcca caccaatgct ataaaccagc gcaaaaccca atgcccgaat
     5641 ccgtttcggc aacagttcag agatcaacaa catcactggc acaccgccaa gcgtggtaaa
     5701 gctgaccatc acgaaaacca ccgtcagcaa catgcctggg cttgggtaat tcaccaacag
     5761 ccagaatgac ggccagctac agatcagcac catcacacgg gagattaaaa tcagcttttt
     5821 acgcccgacc gaatcgcaca acatccccac cagcagcgca cccacaaacg taataacgcc
     5881 cgccagtaac atcgctgcat gagaatagtt ttggttcatt cctaaatact tcgccgccca
     5941 tgtaccgtaa taaaagaggg agatataggt tgcgactgta ctaccaatag cgagcaagat
     6001 accgttagcg atagtcgctt tatgttgcat aagtaaggag aaggcgcttt cgctggctgc
     6061 ggccttctta ttatgtgctg gctcaggaac atcattttcc agactcaaac gcaaccaaca
     6121 gccgacaggc gctaataaca cgccaataaa aaacggcaca cgccagcccc attcagccat
     6181 gacggtttct gaaccggtcg caaagggcag ccatgcactt aaacctaaag cgacaacacc
     6241 tccaaaagtt gttgccaggc cttgagtcgc cagtgaccaa ctgctgtaaa aaccgcgacg
     6301 atttgccgga gctgattcaa ccaacaaaga cattgaggca ccgacttcgc ctcctgccgc
     6361 aacgccctga attagacgcg ctagcaccag cgtcgcggtt ccccaatagc ccgccgtttc
     6421 atacgtcggt gcgatgccga tcatcagtgt accaagactc atcagcgcaa tggtgaacac
     6481 catcgcgggt ttgcgtccga aacgatcggc ccaggccccc acaataatgc cgccgagcgg
     6541 gcgcataaaa aagctcacgc cgaaagtggc aaacgccagc agtagcgcga cgtttttatc
     6601 tgcactgggc ggaaaaaata gcgtgctgat atagaccaca aaaaagttgt aaacgccaaa
     6661 atcaaaaaat tcgagcgcat tgccaaacgc cgaggcaaaa atcacccgcc tgccgctggg
     6721 ctttttcttc acactcacgg tttcatttac gatcgccgtc attccatgtt ccttgccaaa
     6781 aagtgttgtt gtgtgttgtt ttatttattg cagccaggct tctaccagtg cgccccagta
     6841 gctgcttgcc gggaccagac ttgcatcgtt gaagtcgtaa cccgggttat gcaccatgca
     6901 ggaaccttcc ccgacatcgc cattaccgaa taacaggaaa ccgcctttgg gttgggcttc
     6961 cagcatgaaa gagaaatctt cactgcccat caggggcttg atgccaaact cggcccgatc
     7021 ttcaccaaac gttttacacg ccacctgcca ggcgaagcgc gccatctcct cgtcatttac
     7081 taatactggt gtaccgttaa catgcgtaac ctcagccgta gcgccaaagc tggcagcctg
     7141 ggcttgtgcc agagcaggaa tacgggtcag aagaagttgt cgggtatcgc ggctaagaga
     7201 gcggacgctg attttcatct cagcactgtc aggaatgacg ttagccgcct cacccgcaac
     7261 aatgctgccg acggtaatta ccgccgcttc cagcggatcg acattgcgtg acacaatgct
     7321 ttgtaatgcg gtggtgatat gtgcggcaac tagcaccgga tcgatggctt tgtgcgggat
     7381 cgcaccgtgt ccgccacaac cacgaactgt aatatggaac tgatccattg acgccatcag
     7441 tgcccctggc tggaaataaa acttacccac cggcagaccg ggcatgttat gcatgccgaa
     7501 aatgacatcg caggggaaat gatcaaaaag cccctcttta accatgatct caccgccatt
     7561 aatcatctct tccgcaggct ggaagataag acgtaacgtg ccgttaaaac ggcgggtttc
     7621 ggcaaaataa cgcgcggccc ccagcagtat ggtggtatgc ccgtcatgcc cgcaggcgtg
     7681 cattaaacca ggatgtttac tggcccaggg cttgccacta ttttcataaa tcggcagcgc
     7741 atccatatcc gcccgcaggc caatactttt ctctccgtcg cccactttta acgtggccac
     7801 tatccccgtc gtcgccagtc cttgctctac ttcatagccc cactgacgca gcttttccgc
     7861 aaccaaagca ctggttttaa attcttgtaa tcccagctcc gggttttcgt ggatctgatg
     7921 gcggatctca cgcagttctt cctcaaaccc tcgaatatag tgttcgatca tcggcta
//
LOCUS       DAZKFZ010000207         7962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000207 DAZKFZ010000000
VERSION     DAZKFZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(547..687)
                     /locus_tag="ACHOQW_003983"
     CDS             complement(547..687)
                     /locus_tag="ACHOQW_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9574966.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(989..1252)
                     /locus_tag="ACHOQW_003984"
     CDS             complement(989..1252)
                     /locus_tag="ACHOQW_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9574967.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            1677..1802
                     /locus_tag="ACHOQW_003985"
                     /pseudo
     CDS             1677..1802
                     /locus_tag="ACHOQW_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            1867..2127
                     /locus_tag="ACHOQW_003986"
                     /pseudo
     CDS             1867..2127
                     /locus_tag="ACHOQW_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2177..2416
                     /locus_tag="ACHOQW_003987"
                     /pseudo
     CDS             2177..2416
                     /locus_tag="ACHOQW_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2464..3081)
                     /gene="ureG"
                     /locus_tag="ACHOQW_003988"
     CDS             complement(2464..3081)
                     /gene="ureG"
                     /locus_tag="ACHOQW_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFP9574968.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3093..3767)
                     /locus_tag="ACHOQW_003989"
     CDS             complement(3093..3767)
                     /locus_tag="ACHOQW_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFP9574969.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(3768..4232)
                     /gene="ureE"
                     /locus_tag="ACHOQW_003990"
     CDS             complement(3768..4232)
                     /gene="ureE"
                     /locus_tag="ACHOQW_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFP9574970.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4242..5945)
                     /gene="ureC"
                     /locus_tag="ACHOQW_003991"
     CDS             complement(4242..5945)
                     /gene="ureC"
                     /locus_tag="ACHOQW_003991"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFP9574971.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(5938..6258)
                     /locus_tag="ACHOQW_003992"
     CDS             complement(5938..6258)
                     /locus_tag="ACHOQW_003992"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFP9574972.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6267..6569)
                     /locus_tag="ACHOQW_003993"
     CDS             complement(6267..6569)
                     /locus_tag="ACHOQW_003993"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFP9574973.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(6660..7358)
                     /locus_tag="ACHOQW_003994"
     CDS             complement(6660..7358)
                     /locus_tag="ACHOQW_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFP9574974.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            7739..>7962
                     /locus_tag="ACHOQW_003995"
     CDS             7739..>7962
                     /locus_tag="ACHOQW_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9574975.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGG"
BASE COUNT         1916 a         2040 c         2021 g         1985 t
ORIGIN      
        1 cttctgcaat tatcgtttta ttctttcact tttatcaaaa catcaccaga aactggaagt
       61 gacaaccgca ataatgaaca taaaaaggca aatcagaatg gcagtaaatc aataaagtac
      121 catttcatat gaatcatcag aaaaacggtg gacgcgacat ccagcacgag gcaaaacaca
      181 gcaacaaaat aagccggata tcgcgccctg tacttttcta actcaactcc cggcattgtg
      241 caagttttaa tcgcaggtgg attcagagcc acgcccccat tgattcagcc tgaagacacg
      301 tatatccttg gagaaagcag gaatctcatt gctgtgaaat tccttacaga atcttcagcc
      361 ccattttatg gggctttttt tggtgccccc acgctgttac cacactttcg ggatgcgaat
      421 aaaaaagccc acagttttta cactgcggac ttttgttttt tctttgtgat ttgtgtgcaa
      481 gggtgcgact ttcgaccgaa cctttggcaa ccggatagcg gggactgttg tccccgctgc
      541 ggtttcttac ggtttacact gcaagaacgc cgcaatttcc gaccacatga agcttaccct
      601 gaattcacac ggtgatgcgt gtaacaccca gacggtcagc aacatggtca ggcataacat
      661 gcccacaatg atcgtttttt gcggcatagc acttgctcct tttacagaga ggcgctaacc
      721 ttccacttgc tgaaggtaga tggtcagggc ctcgggttaa cgtttaagtt gactcggggc
      781 ctttccacat ccggccttca ggtaatcctt ccagccgtca gccgaaaggc acccgcgcgt
      841 aatgtactgg ttttcagaga ggtccgcaat cttccggtga cgagatcatc tgcacacgcc
      901 ccggtgggac gggctaacgg tatattcagg tgcaggatct ctctgtctgg tgcgttttta
      961 aattgtcaaa cgcaccctgg cggcaaaatc agccaccttt tttcgcatca acaaaacgcc
     1021 caaaacgctg ggtaaagcgt gcaacattac cttctgaagc gacagttctc agtttacctg
     1081 tataaaacgg atgcgactta gaagaaacgt caattgtaac atatgggtaa gttacaccat
     1141 ccagttcaat tacccggtca gttttgatcg tcgaaccgat cctgaagtac tcatcaatac
     1201 tggtatcgtg aaatactact gtacggtatt ccggatggat attcggtttc acaacagttt
     1261 tcccccttta agtgaaaatt ccgcataaag gtacatattc agacggtcag attcaactgt
     1321 ctgttcttat aacctcactc tctttcagta ttacaatatg ttatataatt ataaactgga
     1381 cttcccgaaa aaatccatta aataagaagc gagccgttgc ggaggcttgc tctctccttt
     1441 aacctcatgt gcaatatccg taacgcgctc acataaatca tcatctgata atataacaac
     1501 acccattatt ttattctgac ctaaaaactt cttatgaaat aaacgtttac ccgacgtatc
     1561 tgatcccgct gtttttacct gtatccttta acatacttga gaagcccgct gaaaaaatat
     1621 accgtaaagg cggtttctct gtcttccata aacctgtcgg ataagaaggg cattaaatga
     1681 aaaccaaaaa agttatgatg attgcactgg tcagctctgc gctggctctt tcaggatgtg
     1741 gagccatggg cacagcgatc aaaaaacgaa atctggaagt aaaaacccag atgagtgaaa
     1801 cctgaggtag cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag
     1861 ctaataatga gcagaaaaac ccaacgttac tctaaagagt tcaaagccga agctgtcaga
     1921 acggttcttg aaaatcaact ttcgatcagt gaaggcgctt cccgattatc tcttcctgaa
     1981 ggcactttag gacaatgggt taccgccgcc agaaaagggc tcggtactcc tggttcccgc
     2041 acggtggctg aactggaatc tgaaattctg caactgcgta aggcgttaaa tgaagctcgc
     2101 cttgagcgag atatattaaa aaaagcagct gtagattcaa tctgtcaatg caacacccct
     2161 ttcaattatc tctttcactg cccgcgacgg tatcggttat cagtttgacc tgctggctaa
     2221 cggtgaaacc gtactgcgtg atgcatccgg cgaaaaacgc atcaagggtg aaaaagacgg
     2281 tcgtatgctc atgaatgttg gcctgaatgc ggaaattcgc gacaacatcc gcttcggtct
     2341 ggagtttgaa aaatcggcat ctggtaagta caacgtggat aacgcggtga acgccaactt
     2401 ccgttattcc ttctgataaa aacaaggggc cgcctgccgg ccctgataca gaggatgttg
     2461 tgattactta cccagcatgc cctgctcttc gataaaggcg atgatgtttt gcagaccttc
     2521 accacttttc aggttagtga acccccatgg gcgttctccg cgcatacgta aagtatcacg
     2581 ctccattacc tcgagggagg cgcccacgta tggtgcaagg tcagttttat tgatcacgag
     2641 gaagtcggat ttggtgatcc ccggaccgcc tttacgcggg atcttttctc cttctgccac
     2701 atctatgaca tatatggtaa ggtccgccag ttcggggctg aaggtcgcac tcaggttgtc
     2761 gccgccgctt tccacaaaga tcagctcgag attaccaaat ttctcactca gtgcttccac
     2821 ggctgccaga ttcattgatg cgtcttcacg aatggctgta tgagggcagc cccccgtttc
     2881 gacaccgaca atgcgttccg cttccagtgc tccggcctca gtgagaatgc gttgatcttc
     2941 tctggtgtag atgtcgttgg tgactacggc taattgccag gtgtcacgca tggatttgca
     3001 gagggcttcc agaagcgcgg ttttacctga gccgaccgga ccgccgacgc cgacacgcag
     3061 cggttgcttg attatgttca ttttattttc tcctaagaac ggaataatcg ggaatactgg
     3121 gtttcatgtc gcgcggaagc gatagatgca agtggcgttg ctgaaccaat tgcgtgatcc
     3181 ggggttgcaa gggcactgtc catgttcgcc gcatagcggg cacacaggcg cataattaac
     3241 tgctgggcag ccagctggcc gtagggtacc agcctgacgc ccgccatcac tgcgctttca
     3301 atccagctgt agccaaggct cagggccagg tctgaaacgg atatttgcca gcgtacgccg
     3361 agccatgcca taccggttaa ctggctttgc tggcatagtt tgcgccattc ggccgggcag
     3421 tcgggctgcc agtcgaccag aagtctggtg aaggctgcac cgcggttacg ttcttcatcg
     3481 cgtagctcac gggtttccct gcaggccagc agataagcgg tccagcgccc ggcactgtca
     3541 ggatcgcccg cttcacaggc ccggtatagc cgggcaagca gtggcaggtc gacggcaaaa
     3601 aaactctgtt ccatctgcaa ctgctgccag tgttcaaagg cggcgctatc ttctacccag
     3661 cctgcttcca ctgcccactc cagtccctgg gaccagctgt agcctccaac agggaggctg
     3721 ttactggcaa gctgcatcag ccgcagacgt ttttccggag tcggcattca gtggctgtgc
     3781 tcctgatcgt gattatgggc gtgcgacttg ctggcgtaag cgccggcttc cggctcaaac
     3841 ggaagatgtg caaaatcaac atccagccca aactggcgca gcatatcatc gaggacgtga
     3901 tcgtgatggt agcgcagctc tccgggcatt atttgcagcg gaacgtggcg attgccgaga
     3961 tgccagcagg ctttcgcgag cataaaagga tctgcgcagc gcactacaga aacggcttca
     4021 tcagcggcga tcaccttaat gaattcatca ccgttttcgt tactgagaat atcaccatcg
     4081 cgcagcagca gaccgcgtgg cagcaacagt ccagcctgac gtccatcgtt aagggtgact
     4141 ttaatacggc tttttacccg catgtcaaca gggagggtaa cgctggcagt tgtctgagcc
     4201 ggggtctcca cccgccgggt caaataaagc atttgtgctc cttaaaacag gaaataacgt
     4261 tgcgccattg gcagaatgtc cgccggttca ctggttatca gttcgccgtt gatacgcact
     4321 tcgtaggttt gtgaatccac ggttatatca ggcaacaggc tgttgtggcg catatcggct
     4381 ttttgtactg tgcggcagcc ttttaccaca gcagttgtgc tgtgcaggtt aagctgttca
     4441 gcgacgccat ttgctgctgc tgcctgcgac aggaaagtca cacgacagcg gtgacgggca
     4501 ctgcccaatg cagcgaacat tgggcgatag tgcaccggct gcggtgtggg gatagagccg
     4561 ttgatatcac ccatcggcgc catggctatc attccgcctt tgacgatagt cgccggtttt
     4621 acgccaaaga acgccgggga ccagagcacc agatccgcca gttttcccac ttcaatcgag
     4681 ccgacttcat gagcaatacc gtgggttaat gccggattaa tggtgtattt agcgatatag
     4741 cgcttcacgc ggacgttgtc gttatcacca ctttcttccg gtaacgggcc gcgctgaact
     4801 ttcatccggt gtgccacctg ccaggttcgt aacactactt ctccgacgcg tcccatggcc
     4861 tgcgaatcgg acgaggtgag ggagaacgcg ccaaggtcgt gcaggacgtc ttccgcggca
     4921 atggtttcct ggcgaatgcg cgattcggca aaggctacgt cctcggcgat atccgggtca
     4981 agatgatggc aaaccatcag catgtccaga tgctcatcaa tagtgttgac ggtatagggt
     5041 agcgtcggat tggttgagga aggcagaata ttggggtgcg cgcaggcggt gataatatcc
     5101 ggagcatgac caccacctgc accttctgta tggaaggtat ggatagtgcg cccgccgatg
     5161 gcagtcagag tatcctcaac gaatcctgat tcgttaagcg tatcactgtg tagcgcaacc
     5221 tgaacgtcca tttcgtcggc tacagtcagt gcgcagttga ttaccgcagg tgtagctccc
     5281 cagtcttcgt gaattttgag gccgataacc ccggccgcga cctgctcacg aagcgcatcc
     5341 ggattggagc aattgccttt acccagcaac ccgatattga ccggcaggct gtctgcagcc
     5401 tgcagcattt gataaatgta ccatggtcct ggggtacagg ttgtggcgtt agaacccgct
     5461 gtcgggccag taccgccacc gatcatggtg gtaatgccgg atgtcagcgc ttcttcagcc
     5521 tgctgtgggc agatccagtg aatatgcgta tcgacgccac ctgcggtaac gatcctgcct
     5581 tctgcggcaa taatttccgt ggatacgccg attgggatag tgacgttggg ttgtatatca
     5641 ggattaccgg ctttgccgat agcaaatatc cttccatctt tgacgccgat atcggcttta
     5701 acgatccccc agtaatcgat gatcagggca ttggtcagca ccagatcagc gcagccggcg
     5761 gagagcattt gcccctgtcc cataccgtcg cggattactt taccgccgcc gaatttgacc
     5821 tcttcgccgt aggtagttaa atcatcttcg acctcgatcc acagctcagt gtctgccaga
     5881 cgaattttat caccggtggt agggccgaac atgtcagcat aggcctggcg tgaaatatta
     5941 ctcatcattt gcctccagct cacccattat atctccgcgg aaaccgtgca cgatacgggc
     6001 accgctcagc gccaccagtt caacctcacg cttttggcct ggttcgaagc gcactgcagt
     6061 tccggcggcg atattgagac gatagcctct tgccttctgg cgatcgaact tcagtgccgg
     6121 gttaacctcc gcgaagtgat aatgggaacc gacctgaatt ggtcggtctc cgtgattttc
     6181 aacgataata ctgcaggtag cccggccgat gttaagctct atatagccag gtttaacctt
     6241 atattctcca gggatcatct gttacctcag attatcggat tatggacggt aaccagcttg
     6301 gagccgtccg gaaaggtggc ttcgacctgg atatcgggga tcatttctgg tatgccttcc
     6361 atgacctgct cgcgactcag gacatgccgt ccttcttcca tcagcgcagc gacgcttttg
     6421 ccgtcgcgag cgccctccat tataaaagcg ctaatcaggg ccacggattc gggatagtta
     6481 agtttcaggc cgcgggccag acgacgctct gccagcagtg cagcggtaaa cagtaatagt
     6541 ttgtcttttt ctcttggagt cagttccata gcgttctctt aagtcagcca gatccgggga
     6601 agtaccggtg atttgtcagt cagttgcggc cgcataaatt gccagatatc acgcattacc
     6661 tactggcaac gcagattatc atcacttaaa aagcgaactg tcagtagacc atcggtgagc
     6721 gtggctccgg cgtagttttc cagtggtgtg agtttttcgc gtatgtcttc cagttgcgtt
     6781 tcattcccca ggtagaacag catcgttccc acccatggtt tccgggcaac gcaggtaagt
     6841 tctccattgg ccacgtgcag acgctcaatc aacagcggag aaccgtcgac ccatatctcc
     6901 agacggttag cgagtgttcc gtggctgaag gtttctccga tgaccggacg tccaaggcac
     6961 agaagatccc aggccagcag tgtactggag gacgacagat gaaacacggt atgcaacgtg
     7021 gcgtgagcgc cgggaaaaaa aatcgaatcc tgtggcaacc actccagcgt tgcgttattg
     7081 cacagcgtca ggttttgctg aaggtgcgcc tgcgcgccgc tgctgcggta aaatttactg
     7141 gcaccaggca tggtgatcag cgtatggcaa tcagagtcaa ttattgcgtt gatgatgagt
     7201 tcgtcaccgc cgacgatacc accgggtgga tgaagcagac agaggtggca ggtttcttct
     7261 tcgggataaa aaggacgctg aacagtgagc agaccgacat gatgacgggt ggtgagcgtg
     7321 gttttcccgt gcagacgctg aaaacggaga tccagcatgg cctgccagcc ttttttattt
     7381 tgtggtaacg cggtactgag caatcttacg cccctgataa ataaagataa aacgacagta
     7441 aagagccgga tttccttgtt atataaaaga tgatgccttt ccagtgagtt ttcgtctgaa
     7501 taatacgaat gtttgttttc gtcatgaagc gttccggctc ccggggctat acatcgccag
     7561 aaaagcaatg catattgtcg ggattatcca gagaatcagg cagaaagatc cgacaatggg
     7621 ctgtgattat gccatctggc tcagaatgat atcaaggtgt tatggggata acttgctgat
     7681 acaatagcag tatgacagat tcattaatgc ggaaactgct cactgaaaca gagtgttcat
     7741 gactgagcac tggtcgggtc atgcagatga taatttcccc ttttcttggg gaagcgacag
     7801 tattttcatg catgcctgtc ttcagtcata cggggagagc atctgtgata cagtccatac
     7861 atttgttgcg gacgtcgctc ctgagtcact gactggtctg caaacagttt atctgtacac
     7921 ggcagggctt ctgtgtgata tcgtttttca tatcgagggg gg
//
LOCUS       DAZKFZ010000208         7938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000208 DAZKFZ010000000
VERSION     DAZKFZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.208"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            389..838
                     /gene="alaE"
                     /locus_tag="ACHOQW_003996"
     CDS             389..838
                     /gene="alaE"
                     /locus_tag="ACHOQW_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP9574976.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(875..1219)
                     /locus_tag="ACHOQW_003997"
     CDS             complement(875..1219)
                     /locus_tag="ACHOQW_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP9574977.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1371..1700
                     /locus_tag="ACHOQW_003998"
     CDS             1371..1700
                     /locus_tag="ACHOQW_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP9574978.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1850..3184)
                     /locus_tag="ACHOQW_003999"
     CDS             complement(1850..3184)
                     /locus_tag="ACHOQW_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9574979.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3272..3703
                     /locus_tag="ACHOQW_004000"
     CDS             3272..3703
                     /locus_tag="ACHOQW_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9574980.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3912..4157
                     /gene="nrdH"
                     /locus_tag="ACHOQW_004001"
     CDS             3912..4157
                     /gene="nrdH"
                     /locus_tag="ACHOQW_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9574981.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4154..4564
                     /gene="nrdI"
                     /locus_tag="ACHOQW_004002"
     CDS             4154..4564
                     /gene="nrdI"
                     /locus_tag="ACHOQW_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9574982.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4537..6681
                     /gene="nrdE"
                     /locus_tag="ACHOQW_004003"
     CDS             4537..6681
                     /gene="nrdE"
                     /locus_tag="ACHOQW_004003"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9574983.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6691..7650
                     /gene="nrdF"
                     /locus_tag="ACHOQW_004004"
     CDS             6691..7650
                     /gene="nrdF"
                     /locus_tag="ACHOQW_004004"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9574984.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
BASE COUNT         1993 a         2028 c         1945 g         1972 t
ORIGIN      
        1 taaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca taaaaaatgc
       61 tgtaaatgac ccggtcacga catacaccga attctctacg cttatagcgt tacctcaccc
      121 ccaaactttc attagtgaaa ttaaaggaaa ggtcatatcc aattgattta attgaaaata
      181 aatcatatcg gcatgtaaaa atccacaatg cacaaaaaac gagccgttac ggaatatttt
      241 atctacaaaa actgactaaa taaaaatttt tcactaattg attagtcata gccagcgata
      301 tacgctatgc gaaaatgcag atggcaatga gatccactgc tttcatctcc attaacatcc
      361 cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct tgcgtcatgc
      421 agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt gtattgaagt
      481 tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag cgattccggt
      541 gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc gcgcggcacg
      601 caaagttagc ccgtcgggct ggataaaaaa tctggcggat atcctggctt atgtgacgtt
      661 ccagtcaccg gtgtatgtgg cgatcttgtt agtggtgggc gcagactggc atcagattat
      721 ggcggcggtc agttcaaaca tcgttgtttc gatgttgatg ggggcggttt atggctactt
      781 cctcgattat tgccgccgac tgtttaaagt cagccgttac cagcaggtaa aagcctgact
      841 cttcttttcg cgactggcat cgccagtcgc gtctttaact ggcttcgcca aataaaccat
      901 tcaaataacg ttcaagcgca acacgcgaac taaagccatg cgggataccg taatgctcgt
      961 gtcgctcgcc tgctaaatag agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg
     1021 aagcgggttc agccagtgtc gaactgcgtt cttttaatgt tggatgaatt accagagcgg
     1081 tacgtcccat tcgcgcttcg cgattaacat agacataatt ttccccacgg cggtaaccat
     1141 acgctttctg ggttaccaca tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct
     1201 cgtctggtcg taaatacata tttgatcctc gttattatca accgcgggcc taccttacct
     1261 gattgcgcat tcacaacgct ttcagaaaag tccataaagc cgcgatgaac ctcagttaag
     1321 aaaattatgg tctacactga aaattacatc gaattctaat gggggatcat atgtttaacc
     1381 gaccgaaccg caacgacgtt gatgatggcg tgcaggatat ccagaatgat gtcaatcaat
     1441 tagctgacag cctcgaatct gtattgaagt cctggggcag cgacgccaaa ggggaagctg
     1501 aagccgcacg cagtaaagct caggcattgc tgaaagaaac cagggcgcga atgcatggtc
     1561 gtactcgcgt ccagcaagcc gcgcgcgatg ccgtaggctg cgcggattct tttgttcgtg
     1621 aaagaccctg gtgtagtgtg ggaactgctg ctgcggtagg tatttttatc ggtgcgttgt
     1681 tgagcatgcg taaatcgtag tgagacgatg acaagcagta gtctgacttc tgaaaagtac
     1741 tcagtgcgtc aacgccttgt ccgcctacag gcctgaaaaa catagcgcat caggggattt
     1801 tgccctggtc accagtctta taggaccccg aagcctggct tcggggttat taatttaaag
     1861 cactgcgcaa ttgctgtgct acctgtcgcg ccatactttc gctaacgata ttcgtaaacg
     1921 agagcaataa tcctccgtcc tcgcttgagc ggatacgcca gtcgctaagt gcctgtacgg
     1981 caagaccagc agcattggct ttatgcacga atgtcacgtc attacctgct agtttgatca
     2041 ccatctggat accgcctttt tgcggcacaa cctgaaatcc ctgctgcgta agtgcctgct
     2101 caatccactg ccgtcgctgg gcataatgct ggcgcatttt cttcagatgc cgccagaaat
     2161 gcccctcacg gatgaaatct gccagtgtgt tctgccacag tacgggtaca gcacatggtt
     2221 ccagtgacgc ctgctgccgg aattgcgaaa tctgctccac cggcaccacc agccacgcac
     2281 agcgcaatgc aggaaatagc gctttgctga atgttccggc ataaatcacc cgctgcggcg
     2341 cgtcgagact ttttagtgcc ggtaacggct taccgtgata gcgaaactca ctgtcgtaat
     2401 catcttcaat aatccatgcc tgactacgct ctgcccattc cagtatctga tacctacgcg
     2461 ctaaagagag tgccacaccc agcgggcttt ggtgtgccgg agtaatcagg gcaaaacgcg
     2521 catcaggata attttgtatt ccgcttgtga catccagtcc gttgtcatca accggcacag
     2581 gtaaaatttc cacaccgtgg cgagtgacaa tcgggcgaat cagcggaaag ccgggatctt
     2641 ccatccacat cccgtctccc ggttgcgcca gagcgtgcag aattaaagcc attgaggccg
     2701 cataaccgtg agtgataaag acctgctctg gctgacaatc aatcccacgt gaaacgcgta
     2761 aataatcaac aatagcctcg cgcaaggccg cctcgccgca gacatcgcct aatgccaaat
     2821 caaagcgcgt ctgggtacgc agacggcggc ccatcacccg cgcccacaat tcacgcggaa
     2881 aaagatcaag tgcaggtagc cccatctgaa aaggcagcgg ctgctgactt tcaccagcaa
     2941 aagccacgac atccacagca gactgttgcg gatgtatctg ctcactgaca aaggtacccg
     3001 cctgtccccg cctgaccagc catccctgcg ccaccagctc agcataagca ttttcgacag
     3061 tggatcgaga gacacccagc tcctgcgacc aggtacggct cgaaggtagc cttgcgccag
     3121 gtttcagttt tccttgctcg ataaccgttt ttaactgacg ggcgatatcc tgataacgtg
     3181 gcatatggtc tgctttttta ggtaatttat ggctctcttt agcagtccat tatagtgcta
     3241 cattgcgggc gttaataaga ggagaaactg tatgactaca ttacgtcagc cttactacga
     3301 acttagcccg gcagtgtata acgcactggt gcaggccaaa acggcactgg aaaatagcac
     3361 gctggatacc acgttgatgg agttaattta tttgcgcgtc tcgcaaatca acggctgcgc
     3421 attttgtctg gagatgcaca gcaaagcatt gcgcaaatcc ggtgtgccac aacacaaact
     3481 ggacgccctg gcaggctggc gcgtaagcca tcattttgat gaacgcgagc gagcggcgct
     3541 ggcatgggcg gaatctgtga ccgatattgc cagaacccat gcggaagacg aggtttatca
     3601 gcctttgctt gagcatttca gcgcagcaga aatcagcgac ttaacgtttg ccatcgggct
     3661 gatgaattgt tttaaccgtc tggccgtttc catgcggatg taatttgctg ttgccgggta
     3721 tgcgcccggc aaaaaaacac aaaatcccct accccgttga gccattcacc ggtggcgtaa
     3781 tcaatctaaa attaccatat cttgtgtggt tgaatctttt ttcaactaca tctagtatct
     3841 ctgtatcaac agagagacaa cccgacgcgt atcatcgcgc cgtatcttca ttttaaacgg
     3901 aaatacgaat catgcgcatt actatttaca ctcgtaacga ttgcgttcag tgccacgcca
     3961 ccaaacgggc gatggaaaac cggggctttg attttgaaat gattaatgtc gatcgcgttc
     4021 ctgaagcggc agaagcgttg cgtgctcagg gctttcgtca gttgccggta gtgattgctg
     4081 gcgatcttag ctggtctggt ttccgtccgg acatgattaa ccgtctgcat ccagcgccac
     4141 acgcggccag tgcatgagcc agctcgtcta cttctccagc agctccgaaa acacgcagcg
     4201 ttttatcgaa cgtttaggtc tgcccgcggt gcgcatcccg ctcaatgagc gggaacggat
     4261 tcaggtagac gagccttaca tcctgatcgt gccctcttac ggcggcggcg gtacggctgg
     4321 cgcggtgcca cgacaggtaa ttcgcttttt aaacgacgag cacaaccggg cgttgcttcg
     4381 cggcgttatt gcttcgggta atcgcaactt tggtgaggcg tatggccgcg ccggagatgt
     4441 gattgcccgg aaatgcggcg tgccgtggct gtaccgtttt gaactcatgg gtacgcaaag
     4501 cgatatcgaa aacgttcgta aaggagtaac cgaattttgg caacgacaac cgcagaatgc
     4561 ctgacgcaga aaacgatgga ttaccacgcg ctgaatgcga tgcttaacct ctacgatagc
     4621 gcaggtcgca ttcagttcga taaagaccgc caggccgttg acgcctttat ggcgacgcat
     4681 gtgcgtccga acagtgtgac cttcagtagc cagcagcagc gcctgaactg gctggtcaac
     4741 gaaggttact atgatgaaag cgttcttaat cgctactctc gcgactttgt cattacgctg
     4801 tttgcccacg cacacaccag cggttttcgt ttccagacat tcctcggggc atggaagttt
     4861 tacaccagct atacgttgaa gacattcgac ggtaaacgtt atctggaaga ttttgccgat
     4921 cgagtcacga tggtggcgct gacgctggca caaggcgatg agacgctggc gttgcaactg
     4981 acagatgaaa tgctgtcagg acgctttcag ccagccacgc caacattcct caactgcggt
     5041 aagcagcagc gcggcgaact ggtttcctgt tttttgctgc gtattgaaga caatatggag
     5101 tcgattggtc gggcggtaaa ttccgcactg caactgtcga aacgcggcgg cggcgtagca
     5161 tttttgctgt cgaatctgcg ggaagcgggc gcgccaatta aacgtattga aaatcaatct
     5221 tctggcgtaa ttccggtgat gaaaatgctg gaagacgcat tttcctatgc caaccaactc
     5281 ggcgctcgtc agggggctgg tgcagtctat ttacatgctc atcatcccga tattctgcgt
     5341 tttctcgaca cgaaacggga aaatgccgac gaaaaaatcc gcattaaaac actgtcgctt
     5401 ggcgtggtga tcccggatat cactttccat ctggcaaaag agaatgcgca gatggcgctg
     5461 ttttcgcctt atgacgtaga gcgagtttat ggcaagccgt ttgccgatgt ggccatcagc
     5521 caacactatg acgaactggt tgccgatgaa cgcattcgca aaaaatacct caacgcccgt
     5581 gatttcttcc agcgactggc agaaatccag tttgagtccg gctatcccta catcatgtat
     5641 gaagacacgg taaaccgtgc taaccctgtc gccgggcgca taaatatgag taatctctgc
     5701 tcagaaattt tgcaggttaa cagcgcctca gagtatgacg agaatctcga ctatgcccgc
     5761 acaggccatg atatctcctg caatttaggt tcgttgaata ttgcgcacac catggattcc
     5821 cccgattttg cccgcacggt agagactgcc gtgcgcggtt taacggcagt atcagatatg
     5881 agtcatatcc gcagcgtgcc gtccatcgaa gccggaaatg ccgcctcgca cgccatcgga
     5941 ctggggcaga tgaatttaca cggctatctg gcgcgagaag gcatcgctta tggttcgccg
     6001 gaagcactgg atttcaccaa tctctatttc tatgccatca cctggcacgc actgcgtacc
     6061 tcgatgttgc tggcacgcga acgcggtgaa accttcgccg ggttcaaaca gtcacgctat
     6121 gccagtggtg aatattttag ccaatatctg caagggaact ggcagccgaa aacggggaaa
     6181 gttggcgaac tgtttgcccg tagcggtatt acgttaccta cccgtgagat gtgggcgcaa
     6241 ctgcgcgacg acgtgatgcg ctacggcatt tacaaccaga atcttcaggc ggtgccgcca
     6301 acaggttcta tctcttatat caaccatgct acgtcgagta ttcatccgat tgtggcgaaa
     6361 gtagagatac gcaaagaggg taaaacagga cgcgtttact accctgcccc gtttatgact
     6421 aacgagaatc tggcgctgta tcaggacgct tacgaaattg gcgcagaaaa gatcatcgac
     6481 acctacgcgg aagcgactcg ccatgtcgat caggggctgt cgctgacgct gtttttcccc
     6541 gataccgcca ccactcgcga tatcaacaaa gcgcagattt acgcctggcg caaaggtatc
     6601 aaaacgctct attacatccg gctgcgtcag atggcgctgg aaggcactga aattgaaggc
     6661 tgcgtctcct gtgcacttta aggaatatct atgaaactct cacgtatcag cgccatcaac
     6721 tggaacaaga tatctgacga taaagatctg gaggtgtgga atcgcctgac cagcaatttc
     6781 tggctaccag aaaaggtgcc gctgtcgaac gatattcctg cctggcagac attaactgtc
     6841 gtagaacaac aactgacgat gcgcgttttt actggcctga cgctgctcga cacgctgcaa
     6901 aatgttatcg gcgcgccttc gctgatgccc gatgcactca cgcctcatga agaagcggta
     6961 ttatcgaata tcagctttat ggaagcggtt catgcccgct cttacagttc gattttctcg
     7021 acgctgtgcc agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga aaacgcgccg
     7081 ttgcagcgaa aagcacagat tattcagcaa cattaccgcg gtgatgatcc gctgaaaaag
     7141 aaaatcgcca gtgtgtttct tgaatctttt ttgttctatt ccggtttctg gctgccgatg
     7201 tatttttcca gccgcggaaa gctgaccaat accgcggacc tgatccgtct gattatccgc
     7261 gatgaagcag tccacggtta ctacataggc tataaatatc agaaaaacat ggaaaagata
     7321 tctctgggac aacgtgaaga gttgaagagt ttcgccttcg atttgttgct ggaactctac
     7381 gacaacgagt tgcaatacac cgatgagctg tacgccgaaa ccccgtgggc tgacgatgtg
     7441 aaagcgtttc tctgttacaa cgccaataag gctttgatga atctgggcta cgaaccgtta
     7501 tttcccgcag aaatggcgga agtgaatccg gcaatcctcg ccgcgctttc gccgaatgcc
     7561 gatgaaaatc acgatttctt ttccggttca ggctcctctt atgtgatggg gaaagcggtt
     7621 gaaacagaat atgaagactg gaatttctga gggtgttatt ttcaaaaata tcactacccg
     7681 cgacagggaa ataattcccg ccaaatagct ttttatcacg caaatatttt gtggtgatct
     7741 acactgatac tctgttgcat tattcgcctg aaaccacaat attcaggcgt tttttcgcta
     7801 tctttgacaa aaaatatcaa cttttctcga tttgctctca gcccttatat cacgggaaat
     7861 tccggcgatt tgctcgcatc aatattcatg ccacatttgc catcaggggt tgcctcagat
     7921 tctcagtatg ttagggta
//
LOCUS       DAZKFZ010000209         7837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000209 DAZKFZ010000000
VERSION     DAZKFZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            105..194
                     /gene="kdpF"
                     /locus_tag="ACHOQW_004005"
     CDS             105..194
                     /gene="kdpF"
                     /locus_tag="ACHOQW_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP9574985.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            194..1867
                     /gene="kdpA"
                     /locus_tag="ACHOQW_004006"
     CDS             194..1867
                     /gene="kdpA"
                     /locus_tag="ACHOQW_004006"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP9574986.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1890..3938
                     /gene="kdpB"
                     /locus_tag="ACHOQW_004007"
     CDS             1890..3938
                     /gene="kdpB"
                     /locus_tag="ACHOQW_004007"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP9574987.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3947..4519
                     /gene="kdpC"
                     /locus_tag="ACHOQW_004008"
     CDS             3947..4519
                     /gene="kdpC"
                     /locus_tag="ACHOQW_004008"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP9574988.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4512..7196
                     /gene="kdpD"
                     /locus_tag="ACHOQW_004009"
     CDS             4512..7196
                     /gene="kdpD"
                     /locus_tag="ACHOQW_004009"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP9574989.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVEPPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7193..>7837
                     /gene="kdpE"
                     /locus_tag="ACHOQW_004010"
     CDS             7193..>7837
                     /gene="kdpE"
                     /locus_tag="ACHOQW_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP9574990.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFIT"
BASE COUNT         1598 a         2067 c         2340 g         1832 t
ORIGIN      
        1 ccatttttat acttttttta caccccgtcc gcagattttt gcgaaatctt tgcagccaga
       61 attctaccct tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga
      121 tagccggcgt gttgctggtg tttttattac tgggttattt ggtttatgcc ctgatccatg
      181 cggaggcatt ctgatggctg cgcaagggtt cttactgatt gccacgtttt tactggtatt
      241 aatggtgctg gcgcgtccct taggtagcgg gctagcgtgg ctgattaatg atattcctct
      301 tcctggtacg acgggcgttg aacgcgtact ctttagcgcg cttggcgtct ctaaccatga
      361 gatgaactgg aagcagtatc tctgtgccat tctcggcctg aacatgctgg ggctggctgt
      421 gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccggg
      481 gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata ccaactggca
      541 atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcgggct taacggtgca
      601 aaactttctt tctgccgcca gcgggattgc ggtgattttt gccctcattc gtgcatttac
      661 ccgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg
      721 ggtgctcgtc cctgtggcgt tggtgatcgc actgtttttt attcaacaag gtgcgctgca
      781 aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc tgttaccaat
      841 gggtccggtg gcttctcagg aagcgatcaa gatgctcggc actaacggcg gtggtttctt
      901 taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat
      961 gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag tgacgggcga
     1021 tcgccgtcag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg
     1081 cgtggtgatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag
     1141 cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc
     1201 ggtcgtgact acggcggcgt cctgtggcgc ggtgattgca atgcatgatt cgtttaccgc
     1261 tctcggtggc atggtgccga tgtggctgat gcaaattggt gaagtggtat tcggcggtgt
     1321 cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct
     1381 gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgtg agatgaaact
     1441 gactgcactg gcgattctgg tcaccccgac gctggtgctg atgggcgcgg cgctggcaat
     1501 gatgaccgac gccggacgta gcgccatgct caaccctggc ccgcatggtt ttagcgaagt
     1561 gctgtacgcc gtgtcatccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc
     1621 caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc gcttcggggt
     1681 gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag
     1741 ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt
     1801 gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggaatatct
     1861 ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg ctattcgaac
     1921 caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat
     1981 ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca
     2041 tcgcgatggc aagcggcgcg atgcctggca atgcgctgtt tagcgtggcc attagcggct
     2101 ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta
     2161 aagcgcaggc caacagcctg aaaggggtga aaaaaacggc ctttgcccgc aagctgcgtg
     2221 agccgaaata tggcgctgcg gcggacaaag ttcccgccga tcaactgcgt aaaggcgata
     2281 tcgtactggt agaagcaggc gatattatcc cctgcgatgg tgaagttatt gaaggcggcg
     2341 catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt gaatccggcg
     2401 gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg gtgattgagt
     2461 gtagcgttaa ccccggcgag acatttctgg atcggatgat cgcgatggtg gaaggcgcac
     2521 agcgacgcaa aacgccgaac gagattgccc tgaccatcct gctgattgcc ctgactatcg
     2581 tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgcagtca
     2641 gcgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt ggcggcctgt
     2701 tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg gttgccacca
     2761 gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctgctggat aaaaccggca
     2821 ccatcacact cggtaaccgt caggcatcgg agtttatccc tgcgcagggc gtggatgaaa
     2881 aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg gaaggccgca
     2941 gtattgtgat cctcgccaag cagcgtttta acctgcgcga gcgcgatgtg cagtcgctcc
     3001 atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaat atcgacaacc
     3061 gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tgttgaggct aacggtggtc
     3121 acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcagggg gccacaccgc
     3181 tggtagtagt agaaggttct cgtgtgctgg gcgttattgc gttgaaagat atcgtcaaag
     3241 gcggcattaa agaacgcttc gcccaactgc gcaaaatggg tattaaaacg gtgatgatta
     3301 ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggcgtc gatgattttc
     3361 tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag gcggaaggcc
     3421 gtttggtagc aatgaccggc gacggcacca acgatgctcc ggcgctggcg caggcagatg
     3481 tcgcggtggc gatgaactcc ggtactcagg cggcgaaaga agcgggcaat atggtggatc
     3541 tcgactctaa cccgaccaag ttgatcgagg tggtacatat tggcaaacag atgctgatga
     3601 cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta
     3661 ttccggcggc attcgcggta acgtatccac agttaaatgc gctgaacatc atgcgcctgc
     3721 attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt
     3781 tgattcccct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc
     3841 gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag
     3901 tcattgattt actactgacc gtttgcggtc tggtgtgagg tttaccatgc gtggattacg
     3961 tccggcatta tcgacattta tctttctgtt attgattact ggcggtgttt acccgctgct
     4021 gaccaccgcg ctggggcaat ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga
     4081 aggtgatacg gtgcggggtt cggcattaat cgggcagaat tttaccgaca acggctattt
     4141 tcatggtcgc ccgtcggcaa cggcagaaat gccttataat ccacaggctt ctggcgggag
     4201 caatctggcg gtcagtaacc ctgagctgga taaactaata gccgcacgcg ttgctgcatt
     4261 acgggccgct aacccgaatg ccagcacgag cgttccggtt gaactggtga cggcgtcggc
     4321 aagcgggctg gacaataaca tcaccccgca agcggcggcc tggcagatcc cacgcgtagc
     4381 gaaagcgcgt aatctcagcg ttgaacagct cacgcaactg atcgcaaaat acagccaaca
     4441 accactggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca atctggcact
     4501 ggataaactt gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa
     4561 ctgccgcgcc gcatcggggg aagctgaaag ttttcttcgg tgcctgcgca ggcgtcggga
     4621 agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg gatattgtgg
     4681 ttggcgtggt agaaacccac gggcggaaag ataccgccgc catgctggaa gggctggctg
     4741 ttctgccgcc aaaacgcctg gcgtaccgtg ggcggcacat cagcgagttt gatctcgatg
     4801 ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac agtaatgcgc
     4861 caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa gctggcattg
     4921 atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg
     4981 tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg
     5041 tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct
     5101 atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc aatctgatcg
     5161 ccctgcgcaa actggcactg cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct
     5221 ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc cttttatgca
     5281 tcggccataa caccggcagc gaaaaactgg tccgcgcggc ggcgcggctg gcgtcacggc
     5341 tggggagcgt ctggcacgcg gtgtatgttg aaccccctgc cctgcatcgc ttaccggaga
     5401 aaaaacgtcg ggcaattctc agcgccttac gtctggcgca ggagctgggc gcggagacgg
     5461 caacactttc tgatccagcg gaagagaaag cggtagtgcg ttatgcccgt gaacataatc
     5521 tcggcaagat tattctcggt cgcccagcct cgcgccgctg gtggcgtcgg gaaacgtttg
     5581 ctgaccgact ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac
     5641 cacccgcccg cacgattaac aacgcgccgg atagccgctc ttttaaagac aagtggcggg
     5701 tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc ttaattgcca
     5761 tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatcta cttggcgtgg
     5821 tggtggtggc gctattttat ggacgctggc cttcggtggt tgctaccgtc attaatgtgg
     5881 tgagtttcga tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat
     5941 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgcgg
     6001 gtgtgcgtta tcaggcgcgg gtagcccgtt accgcgagca acgcacacgg cacttatatg
     6061 aaatgtcgaa agctctggcg gtgggccgca gtccgcagga catcgccgcc accagcgaac
     6121 aatttattgc ctccacgttt catgcccgca gtcaggtgtt gttgcccgat gacaacggta
     6181 aattgcagcc gttaacacat ccgcaaggaa tgacgccgtg ggacgatgcc atcgcgcagt
     6241 ggagttttga taaaggcctg cctgcggggg cgggcaccga cacgttaccc ggcgtgccgt
     6301 accagatttt gccgttaaaa agcggtgaga aaacctacgg gctggtggtg gtggaaccgg
     6361 ggaatctgcg tcagttgatg atcccggaac agcagcgcct gctggagacg tttacgctgt
     6421 tagtcgccaa tgcgcttgag cggctgacgc tgaccgccag cgaagaacag gcgcggatgg
     6481 caagcgaacg tgaacagatc cgcaacgccc tgctggcggc gctttcgcat gatttacgca
     6541 ctccgcttac ggtgctgttt ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag
     6601 gatcacccca cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac actacccgac
     6661 tggtgaataa tctactggat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag
     6721 agtggttaac gctggaagaa gtagtcggca gcgcgctgca aatgctggaa ccgggtttat
     6781 cgtcgcccat caatctttct ctgccagaac cgctgacctt aatccacgtt gacgggccac
     6841 tctttgaacg ggtgctgatt aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg
     6901 ccgaaattgg tatcgatgcc cacgttgagg gcgaaaatct acaactggat gtctgggata
     6961 acggccccgg tcttccgcca ggccaggagc agacgatatt tgataagttt gctcgcggga
     7021 ataaagagtc ggcggtaccg ggggtagggc ttggactggc aatttgtcgg gcgatagtgg
     7081 atgtacacgg gggcactatt accgcgttca accggccgga aggtggtgcc tgttttcgtg
     7141 ttacacttcc ccagcaaact gcccctgaac ttgaagaatt tcatgaggat atgtgacaaa
     7201 cgttctgatt gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg
     7261 cgacgggatg cgcgtctatg aggccgaaac gctgcaacgc ggcttgctgg aagcggctac
     7321 ccgtaagcca gatttgatta ttctcgatct cggcctgccc gatggtgatg ggattgagtt
     7381 tatccgcgac ctgcgccagt ggagcgcggt gccggtgatt gtgctttccg cacgcagcga
     7441 agagagcgac aaaatcgccg cgctggatgc cggagcggat gattatctaa gtaagccgtt
     7501 tggcattggc gaattgcagg cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc
     7561 cgcgcccgat ccgctggtga aattttccga tgttaccgtc gatttagccg cccgcgtgat
     7621 tcaccggggt gaggaagagg tgcatctcac cccgattgag tttcgcctgc tggcagtgct
     7681 gctcaacaac gccggaaaag tgctcaccca gcgccaactc cttaaccagg tgtgggggcc
     7741 aaacgcggtc gaacacagtc attatttgcg tatttatatg ggacatctgc gacaaaaact
     7801 ggaacaggat cccgctcgcc ccagccattt cattact
//
LOCUS       DAZKFZ010000210         7808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000210 DAZKFZ010000000
VERSION     DAZKFZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..4772)
                     /locus_tag="ACHOQW_004011"
     CDS             complement(<1..4772)
                     /locus_tag="ACHOQW_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9574991.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWT
                     SGTHYTGGSKRLDAGIIDITSPTNQYGLHPVIEGFDITL"
     gene            complement(4792..5253)
                     /locus_tag="ACHOQW_004012"
     CDS             complement(4792..5253)
                     /locus_tag="ACHOQW_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP9574992.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5281..7182)
                     /locus_tag="ACHOQW_004013"
     CDS             complement(5281..7182)
                     /locus_tag="ACHOQW_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9574993.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENHTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1529 a         2325 c         2049 g         1905 t
ORIGIN      
        1 agtgttatat caaatccttc aataactgga tgtaatccat attggtttgt aggagatgtg
       61 atatcaataa taccggcatc tagacgtttg gaaccacctg tataatgagt gccggatgtc
      121 cacttaccat ttactttaaa tggtccattc gttgtcgcag gaactcgctc tccatttttt
      181 atggctggtt gaatctcaac tctaaattta ctacctgtta attctagtct tttatttatc
      241 atgtttatgt atttattgaa tgcagcctct gattttatcc aatcaccatt agcatattta
      301 agtgctctat tccctaatga gttcaaaaca tcagatatgt tgctttgctc actcccccat
      361 ttcgtccagc gggctaactg acatatagcc agccccaaag gatcgatata cccacaagga
      421 ttcggcacat accgataagt ctgctcccct ccctgcaacc ccagcggatc actcaccagg
      481 tacatcccgg tttccggctc gtagtaccgg aaccggttat agcacagccc cgattctgca
      541 tcctgccact gtcccgcata cagcaggccg gggtccgctt ccgggtcccg ctccccgcgc
      601 gggtccgggt agtctgtgtc tgcgggcagg ctgagtgcca gcccccacag gctggtctgc
      661 cccggccgcc agacggtttt accttcactg ttaaagagca tcagcgggcg gcccgtcagg
      721 tcgctcaccg catgattcgt tcgcgtcacc cactgcgggg ccacggacgg atgcggctgt
      781 cgtacccggc tgaactgctg gcttatcagc tcaaagccgt taaacaccag gtgccttacg
      841 ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa tactgtcgcc gtcccacagg
      901 tacgtaaaac ggattttctt ccggtcgcag cgtttttccg tccgtctgcc gctggcgtcg
      961 tggcggtatt cccactgctc cccctgtgcg ctgcgatacc cggtcagctg gtcccggctg
     1021 tcccaccgga accgctctgt ttctgggcgg tagccgtcac ggtgttttgt gcggctgacc
     1081 atccggcctg cggcgtcata gtcatactgt gtgtttccgg cctgtttcag ccggtggcct
     1141 gcggcatact ggtcagtctc tccgcttatc cggtgtctgc ccgcagactg cgccttcagg
     1201 tagccgcagc tgtcatagcc ataagtctcg tcgccttcgc catagcccag cccggtgcct
     1261 gaggctgtca ccgccgtgac ctggccgttc cggtcaagcc ggtactcccg gtgtgtttcc
     1321 cgtccgtaat cttcccggct ggtgacgcct gatacgttac ccgcgctgtc gtaacggtat
     1381 tcccgtagca gtgaagggag cagtccgccc catgtggctt ccgccgggaa aagtccggcg
     1441 cgctggctga caggctgacc ggctgcattc cagccctgtt cacaggcaaa accccggtta
     1501 ctgtgccgga gcacttcacg tcccgcctca tcacggctga acgtcagcgt ctcatccccc
     1561 ggcaggataa cctccgtcag ttctccggcg gcgttgtagc ggtatgtggt tttcagcagg
     1621 gatccgtcgg ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg
     1681 tgttcgtcgg ggtagtggta ttcaatggcc cgtcggccct gagtggtgac attcacccgc
     1741 ccgcgttcat catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc
     1801 ctgccggatt tgtcatacgc atactgcgtt ttgccttccg gagtgatgac ttctgccagg
     1861 cggcctgcgg catcccggct gtagaggatg gtttcctcca ggccgttgac cctggcggta
     1921 cacaggccat cagcgtcata acggtaacga cagaccacgc cgccccagtc acgttcttcc
     1981 gtcacccggc cacagttgtc cagaatataa agataagact cgccctgcgg gttaatgact
     2041 tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc agcggtggcc ttcaccgtcc
     2101 gttcgcgcca ccggcaaatc aaacgggccg tactccattg tccagacaac gccgttaccg
     2161 tccgtgtacg ccagcaggtt gccgtggcgg tcgtagcgga atccttccgc gccggttcct
     2221 gcccgctcaa ggccactcag tttatggtgc tgctgccact gcaaccgcac tgactgaccg
     2281 ccggggccgc gcatcttcac cggcaggtcg tgaccgctgt attcggtgtg gtgttcgcgg
     2341 cccatcacgt cggtgagtgt ttccggccag ttgtggtggt cgtactctat gcccagacgc
     2401 cgttcatcct ggcggaatat ccccgacagc agcccctgag gggtgtacgc cagccgggtc
     2461 tcttcgccct gtggcgatac cagactcacc ggcaggccgc gttcatcaca ctgctgcgtc
     2521 cacgtctgcc cggcaggggc ggtgatgttg ctgagcaggc cgcgctcatc atagcagtac
     2581 tgccaatccg cgccgtctgc cgccgtgtaa cggcttaact gaccgtgtgc ggcgttgtat
     2641 tcaaacttct ccacccgccc ggcaggggag tggcgggcca gcaggtgatg gaattcatcc
     2701 cattcatacg tggtggtgct gccgtccggc gcttcttccc gcagaatatt gccgtcagga
     2761 tcatagtaat aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc gtcaccatag
     2821 tccagcgtgg cgttgtaata gccctccgcc ccggtgacgg tcacgcaccg gccctgtgca
     2881 tcgtaggtga aacggctcca cgtctggtcg ttatcggacc agcggatgat ccggtccgca
     2941 gcgtcgtact cataaaacag gtggtagtcc agccgcgcat cctgttcgta ggtcgccagc
     3001 gtctgtatgc cgttatccgt gcggtgaatg gctttcaggt agccgctggc gtggtacgtc
     3061 agtttaagcc ggatcccgtc ggtgtgactg acacccgtga tccggtactg ggtgtcgcgc
     3121 tcaaacgtga tgcggttgct ggcggtgtcg ctcatcccgc ccagcatgta catccggtct
     3181 gcggggctgt gtgccaggcg gtcatcgctg gtatcgtcag tcaccgggtg gcgcagacgc
     3241 attcccggaa aggcgtcata aaagaagcgg ctgctcagtg catccctgtc gaacagctcc
     3301 agataatcat cacggcggcg cagaatgtac tgggcgtgat acgggttaac cgccgtatca
     3361 ctttcaccgt cgaaggcgaa gttcagcgtg gctcccaggc tgatgacatc cacccggttt
     3421 ccctgttcac gcacccaggc gtattcgctc caggagtcgc tccagcccac gcccgtcagg
     3481 ccatgcagcg gcagtacaga actgtggaaa cgggtgaagg agagggggag tgtctggccc
     3541 agcgtaaagt cggtacgttc gtcgcagtat gcgccggtta ccggatcgac aggatctgtt
     3601 acccatctga tgactttatt ggcaatactt tttaacgaat tacctgtttt ccccagcaac
     3661 gcgccgagct ttgcgcccag cagcttaccg cttttcccca gcaggtttcc cagtttgccc
     3721 agtttcgcca gattccccag ttttcccagc agtcctgctg gcggcagaag atagctggct
     3781 atcgttattc ctcccaggat cattctctgc catggcggga actccggctg gatatccaga
     3841 taggtgacct gctcaccccc aataaagacg tccggcgaac cttccacgat tgccgcgctg
     3901 cactccagtt tgtcgccctt gcgcgcggca ggctgattgt tgataaagac gttgctgctg
     3961 ccctgggcta tcctcggtgg tttgttctcc ttgtcgcaga gcgccgtgtc ctcaccggct
     4021 tccgggaaat ccacatcttt tttgatttcg ccacgcccgc ccaccatggt ggcaaactcc
     4081 attaataccg gaccggcggg aaacagaagc gatgcgccgg cgctgacgcg atccataaaa
     4141 ccggcattgc tgtccgtcag ggtgataaca gcgttccccc aggccttcgc cagtggtact
     4201 gctttttccg ctgccgattc aagcaggtca gtggcaatat caccccatga gggttccccc
     4261 tgtgggactt caggtattgc tccaggtggg ggaggagggg gaagggtgac ggctgcacgg
     4321 gctgctggct ttccgttaat ttttacattt ttagaaccgg tattgatggc gccgtccgtg
     4381 tcacaaaagc tgtttatcca tgattcaagc tggttctgtt tctcctccac ataatcactc
     4441 gccttatagc tggcatacat cagcgcaggc accagaacca gcgcataagg gccggtggca
     4501 caggcggcca taatcaggca accttctgct atggtcacta tcgcccccag cacggcccct
     4561 gccagcgccc ccaaaaaact cttgtgctga atttcatcgc ccagcctggc ggcggggggg
     4621 ccagactttt ttgatgccaa catctgtgca atggctttgt cgttggctgc ccgtttcatc
     4681 tggctggcgg ccatcgcccg ctgcgacagc atccgcattg ccagcgtacc gcctgcggtc
     4741 gctccctgtg gcccgcctgg tccttcactc atgttttatc cctcgtttat gtcagcgcgg
     4801 ctggtccttt tggatgtcat tctccggtgg caacggggtg aagctcttta tcacctccag
     4861 cagcatcgtc ttttgactgt tcttcaggga taaggaggtg ctgaaagtga aggtcagcag
     4921 cgtatctcca cgccgctgga atacggctat ctggctaatc cgcccctgat tcccttcatc
     4981 ttccggaccg ttccaggaat aatccagcag ccaggccggg gcctctgcca gagtgatttc
     5041 ccggcaggca tgttcggtgt acccatcaag tttgccctgg tacagcgcca gcgtttcctg
     5101 gtacagctct ggtgtcaggg ttccctgtga aacgggttcg cgggtaatga ccaggttgat
     5161 gccactgtta tccgtggata ccaggacctg catcgactga tcctgccatt gcgatggcag
     5221 ttgaatatga ccttcattaa aagtgaactg cataaaatac tccctgttaa tctgagtgaa
     5281 tcagttgatg aaaacagaac cgcccacgat ggtgatgcca tccttcgaca gcctgattac
     5341 cgttgattcc ccgacctgta gctgaatgcc atccggcgtc agcgcaattt tgctttcctc
     5401 cacggccagc ataatttcct taattgccgt ataagcctgt ataccgttgc cttcgccacc
     5461 attgtttaca gcgtcaacat gcaccgaacc ggcaatcgtg cctctgtcct gaaagacatc
     5521 ctcagataaa gaccctttca gtactccggt gatcttgtgg cctttttcca ccgtaagaag
     5581 gcggttacct tcctggatcg tgaccgtctg gtcatttttt acggtttcgc gatgatcgtg
     5641 ttttacggtg gtggtacggt cgttgagcac ttcggtatcc atgttcttct gcgcgtggat
     5701 gtagacctgt tctctccctg tcgcatcatc aaacttcagc tcattaaacc cgctgcccat
     5761 atacgttttg gaacggatgg tcatctgcgt cttcgtcccc ggcaggctgc cgggggtgtg
     5821 gttttcctgg tggtaggtgc gccccataat gatcggctgg tccggatcgc cgttgaggaa
     5881 gtccactatc acctcctgac ccacacgcgg tatcgccagg ttgccaaaac cggtgcctgc
     5941 ccacgcctgt gccacacgga tccagcacga gctgtcctgg tctgccgggt tataacggtc
     6001 ccagttgaat ttcacccgca ctcggccatg ttcatcacag aagatttcct caccctccgg
     6061 ccccgtcacg acggcgctct gcggaccgtc caccagcggt tttagccgcg gctggggtcg
     6121 ccatgtccgg tctgccggga tcaccgcaaa atggttctcc agcgccgtcc ctgctccccg
     6181 ccttcccggc accgcctgcg gctgctcgcc gtgcagttca cttgccacca cctgccattc
     6241 ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt cgccgtcccg gccatatctc
     6301 cggcgagcga ctcattcccc gcgcggtttc tgcattgttt cgccagccct ccatctgcca
     6361 gcgggcaaag ttctgcccgt gggcgccctt gaaacgtccg gggtagtcat acacctcata
     6421 ctgcgtgcgc tggtaatcct ggtgctggcc ttcctgatca aaacgtcctg cccagccggg
     6481 gcgtttaaag gtgtagtctt tggtcaccac ggaagaaggg cggatttgtg cgctgtagcg
     6541 aaactggctg atgcagaggg tgctcacctc ggtacgggtg ttcgggttcc aggggatttc
     6601 aaaagattcg ggcagatggc ggaccgtgtc gcacagcacc aggctctggt cggtactttt
     6661 gtaagcatgc tcctcataaa agaagatgcc ttcctccgcc gccatccggc acaggaaatc
     6721 gtaatcagtc tcaccgtact ggacacaaaa ctcacgggaa ggatgcggtt cactgaacag
     6781 cggactccac tcggtcaccc cgttttcctg caatatcgtg ccgaggatgc ttttgatgtc
     6841 ctcgttctgg aagatacgga agttctggcg cagaccggca cgccacagcg gcgggtgcac
     6901 cttcatgctg tacagcatct ggtttttgtc gttctccccc agctcaaacc aggtcaccac
     6961 acctttcacc cggcgctgta catcatcacc ctgccatatt gtcaggtagg ccattttgtc
     7021 cagcacctgc gcaaattcaa gggagagaaa ctgctggctg accaaggaga gatcgaggga
     7081 aaagagcgaa gagagtgact gggtgagatg aaaggaaacc accgcaaaag cgtccggtgg
     7141 caggccgtcc acttccagtg tgaaacgtaa tccggttgac atagcaacct ccggtaaaat
     7201 gaataataat ccgcaccgta caactgcgga taatgagata agcgggacga cttaagacgt
     7261 tattagggaa ggaacaaaaa atgctatgat tatgtgagga ttttgtttaa atattacttt
     7321 acagaaacgg gtgtctgttc agtcaaaaaa ataatcacaa gctcaactta ttgtttcctg
     7381 acgttattca ggacaggtgc gcacatcaca gcgagtaaca aaaacgcaaa gttaaaccaa
     7441 tattcataat atgtttttat tcacaactaa ataacccaat taacatcatt aaaatgaatg
     7501 attgcttaaa aaatatatgc taccgctcca ccctgataac atgatctgca cattgccatc
     7561 ttaacgtcgt taaacactgg cttaatagtg gatatttcaa attaggaatt tattaaattc
     7621 acctgatgaa tatcataagc aaatagcata gaaaataatg acaactcccc cgaggggacc
     7681 atttccaaag cactgcacca ccacctggtg cccggatgat cttgcttcag tggtaagagt
     7741 acctgattca ttgttcccaa aatggtgttt tcccctgccc ctccaataat gtgcagctct
     7801 cacgtgat
//
LOCUS       DAZKFZ010000211         7672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000211 DAZKFZ010000000
VERSION     DAZKFZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..589)
                     /locus_tag="ACHOQW_004014"
     CDS             complement(<1..589)
                     /locus_tag="ACHOQW_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9574994.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWIGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKV"
     gene            790..1044
                     /locus_tag="ACHOQW_004015"
     CDS             790..1044
                     /locus_tag="ACHOQW_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9574995.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(1094..3064)
                     /locus_tag="ACHOQW_004016"
     CDS             complement(1094..3064)
                     /locus_tag="ACHOQW_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9574996.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLTFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRKRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFVEEGRNFWAGVEYTF"
     gene            complement(3090..3944)
                     /gene="modD"
                     /locus_tag="ACHOQW_004017"
     CDS             complement(3090..3944)
                     /gene="modD"
                     /locus_tag="ACHOQW_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFP9574997.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(3941..4753)
                     /locus_tag="ACHOQW_004018"
                     /pseudo
     CDS             complement(3941..4753)
                     /locus_tag="ACHOQW_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(4763..5521)
                     /locus_tag="ACHOQW_004019"
     CDS             complement(4763..5521)
                     /locus_tag="ACHOQW_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9574998.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            complement(5518..6498)
                     /locus_tag="ACHOQW_004020"
     CDS             complement(5518..6498)
                     /locus_tag="ACHOQW_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9574999.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(6498..7520)
                     /locus_tag="ACHOQW_004021"
     CDS             complement(6498..7520)
                     /locus_tag="ACHOQW_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFP9575000.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLSEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRQGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAT"
BASE COUNT         1837 a         1942 c         2020 g         1873 t
ORIGIN      
        1 tcactttgcg ctcgctgatg gagattaact gggcgtcaaa gtgaaaaaca ccccattgcc
       61 ccatgttgac ttcaatctga gcgaagcgca taccttcgtg caacccggca ggttttgctg
      121 tttccagtaa tgcgcccatg ccgcctaacg acaaatcata caggcggaaa cgtaacgtac
      181 tgttatccgc cagtttggtc tggcaaaaat aaggcggatg gagtggggcg gagatgcgga
      241 aatatcggcg tcgttgtaca aaccataagg tgggaggcgg tacggtaata aatgccggaa
      301 gctgcaagta ttcactctgc tgtagttgtt caacagtaaa ctcgactttc gcaccctgag
      361 tttcggcggt aatggtaatg tgctgtgcct ttagcacggc gatgttgtct tcggcttgac
      421 tgccgaaatc cagcaccagt ttatccgggg ttattaccag tattttgctg atcagctgcc
      481 cgccaatcca actgagacgc aaaggaattg cggctttgtg caaatcgcgt aacacgccca
      541 ggacggctaa cggattttgt ttcaggaact gctcatggta atgactcacg cggaaaaact
      601 cctgactgac aaactgtctt tggtttatcg gtcacagtta acaaaactta atacaaatgc
      661 gtgaatattt tttacatgtt gttcttaaat cagccgacat acgcccagca tcgatcccgc
      721 ccttgcctat acttagagcg ttgacgtaag catttcttgc gtccgattca tcgaaacgag
      781 ggcctgaaca tgggaattat tgcctggatt atttttggcc tgatagccgg cattatcgcc
      841 aagctaatca tgccggggcg tgatggtggt ggatttttcc tgacctgtat tctcgggata
      901 gtcggtgcgg tggtcggcgg ctggctggcg accatgtttg gcattggcgg ctccatcagc
      961 ggttttaatt tgcacagctt cctggtggcg gtggtgggag ctattctcgt tctgggcgta
     1021 ttccgcctcc tgcgaagaga ataagatttt cataaggcgg atagcgatac agatgccgct
     1081 atccgctttc acatcagaac gtatactcga cacctgccca gaaattgcga ccttcttcaa
     1141 caaacccttc actgtaggca tattgggtat caaacaggtt attgaccgac gcattgacgc
     1201 tgaagccatg acctaaggtg taatcggctc gaatgtgggt caccgcaaaa ccggcggctt
     1261 tttgtgaacc gtcactgtta ctgtagctgg aggaacgcgc ctcttccgac agcgttacgc
     1321 ttaacggctc ccacggtttg agagtcatcc atgcggtcat tgtctgcgtt ggcagatcgg
     1381 ttatcttgcc gatgtcttta cgtttggcgt cagcgtggat cagggcgtag ctcaatccta
     1441 catccagtat attgctgatt ttccccttaa taccggcatc cagaccgctg taatccaccg
     1501 tgccgctgtt ctgattttga atggtatcgg catcgatatt gtgcgagagg atggcatcac
     1561 tcacccggtt atagtaaacg ctgacctcaa atccccagtc gtgcgtgaag gcaccattcc
     1621 aggttaaatc caccccgcga gcgcgttccg gcttgagctg cgggttaaca atcgctatct
     1681 ggttatacgc aggtttggat gtggtatagc gttctttcag cgtcggaaag cgtttgcggt
     1741 catagtacga aagcgccagc gtgtcttcat tgacaaagtg gtatttcccc atcacctgcc
     1801 agttaaaagc tgactgattg ttgtcgtcat aatgggtgat gctgccatct ttctcgtgtt
     1861 ttttcgcttc tacgctatcg cgccagtcat agctgattcc agccacaaca tcgacattat
     1921 cggcagcagc ccattgatat tcactggcga gcgaccaggt acgatcttca tagcgatcgt
     1981 aagcggcgtg cggcgcacct ttttcccggt gtacgtcatc tttccagtta acggcaaacg
     2041 tcagcagatc gttttcacgc acatcggctg ccagttgtaa tccggcaccg tcgctgtaat
     2101 cggaataatg gctgtagctg ccttttttat ttttcaaatc agccagcgag ttgtacatca
     2161 tcagcgtatt ttcaaaggtg tcgcgataca gccgactttt cagggtaaaa cgatcgttta
     2221 gttgggtcgt tccctgataa taaaaacttt ctttgtcata ctctggccac tgccagtagc
     2281 gtgatttttg accactattt ccgctgtatg gcgggttatc tttttcacca tcctgcttaa
     2341 tgtaagtcag tgtgtattca tcgttttcac gtggtgtaaa tccgagcttc acaatgccgc
     2401 gtttatcatc agccgatgaa ttaatcatct tgccgtgttt gcctgcaata tcattattta
     2461 caccatgcgg caggccgaga aaatcctgct ttagctggct accgctgact tgcaaatacc
     2521 ccagttcgct gctggcggca aatgaagcat gcatatcata ggcattgtcc tggctacggc
     2581 tccatccctg gcgatatccc agacttgctt ccagaggttt tgttggcttc tgggtggtga
     2641 tattaatggc tccgcccatc tgattaggtc cctgaagcag cgacgaatac cctttggaaa
     2701 cttcgactgc ccccagattg ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg
     2761 gaacataaat gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa
     2821 cttttacctg ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac
     2881 ttaatgcctg agcgacattc tgtttatcca gggccttcat ggtgggttga tcgagaacgg
     2941 tggtcgtcgt cgatgatacc ggactggacc atacggtcag cgagtcgctc tcctgagccg
     3001 ctacggcctg ctgggtaaag gcaagtgata atgctgtgca gagataatgt tttttgagtc
     3061 tcatagaata aattccgttt aacaaagaat taaatagagg ctgcgggttg cagactgact
     3121 ttaatgtcag ccggtgccgc gtaataaggc gcggaggtaa taaagaggcg aatgccgcag
     3181 tcgaggtaat ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtgg
     3241 ggagccagcg acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg
     3301 agctgaagta cgtctggttg cgcgcgtaac gcggcgattg cctctttcgg cgtgtcggct
     3361 tcaacaacaa ttttcttctc gggtgcgtgg cgacgtaact gattgattgc gcctgaccag
     3421 tcctgattgt catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat
     3481 ccggcacgat gaatcagtcc tccggcagcc agaattgcct gcgaggccag taagcgggtg
     3541 cccggaattg cttttcgggt gcaggcgata ttgccatcag gataacgttc acgaagtaac
     3601 gccagcattt gatcgagata atcagaaaca ccgcaactcc actccagcac attctggatt
     3661 gccttccatc cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgctgaccc
     3721 gcgttcgctt gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac
     3781 atcttacacg cgacagaaac gccgctgacg cagccgccct gacggagaaa aaactctata
     3841 tagccatgct ggtgtccaat atttaatgct ctcgtggtca ggtcaccgcc ctggatatct
     3901 tccagaagta acgcatcgat ttgtgcctga gaaaggaaaa tcatcgcagt gcctcctgcg
     3961 gtacgctatc ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat
     4021 cttgttgttg ataccagcga tataaccgct cacgctcatc gtcgttaagc gcgccaagac
     4081 tccagctgac gttctgctca aaacgttcct aggtgctgtt atcctgttgg caattttgtc
     4141 cacggataaa atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga
     4201 tatagttggg taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga
     4261 acgaggtgct gaccagatga gtcgtataga cgcgaagccg tgcctgatta ttcagtttgc
     4321 tcattgcctg gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc
     4381 gtggcaggtc actccagtcc tcttcccagg cgcgctggat caagtggaca ttgtctgcct
     4441 tcagcgcggc ggcgcgacgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat
     4501 aaatcgtcgt gagtttgtca gccagcgcca gcgatacggt acccggtccg cagcccatat
     4561 cgaataaggt ctgagcgcct tgtaggtcaa ttttagcgat cagttgttgc aggtagctgt
     4621 ccgtaggcga ggcgcaattt tccgccattt tttccgcccg ttgatcccag tgatccggcg
     4681 ttttttctgt gcggtgggcc agcttgagtt gctgcagata aaggtctgca aaatcaatat
     4741 catcaatgag catgattttt ccttagtggc taattgcaga gaggtgatga tgaatctggt
     4801 cggcagaaac ccgatacagg gcagccagtt tgtttgttgt taactgttcg gtcggtaagc
     4861 cttgtgttac tcgtccatct ggctctacct ggataatgct gtcggcaatg gcgttggcat
     4921 ggagcggatg atgagtcgac attaacattg tcatgccgtt atttttgagc tgtgccagcg
     4981 tgtccagtag ttggatctga tgaccgaaat cgaggctgga ggcaggctca tccagtaaca
     5041 acaagcgcgg ttgctggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc
     5101 taagcgtatt ccagcgtcgt gccgccagat gcaaaatgcc cagcttttcc agttgctccg
     5161 tggctttcag gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggctaggc
     5221 ccatcaacac catatccagc acactaaaag caaaaatacc gtcgtgcgcc tgcggcaccc
     5281 aggcaatagc ctgcgcgcgc tggcgatggc tgagtgtctg cacagggagg ctatccagta
     5341 agacctcgcc ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc
     5401 cgcaaccatt ggctcctaac agacaacaaa ttgttccttg cgacagggtg aaggacacgt
     5461 cgcgtagcac cggatggcgg gagtaaccca gagacgcatg acggacggca agcaaagtca
     5521 ctggcgacct ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag
     5581 aatgccaagt gggatttctg ttgttccaat gctgcgtgcc agcgtatcgg taagcaggag
     5641 cagaatcgcg ccagtacaca ttgccatggg caacagctgc tgatggttat gaccggtcaa
     5701 caagcgggca atgtgcggaa cgaccaggcc gacccaacca attatgcccg ctattgctac
     5761 cgtactggcg gtgattagtg tggcgcagac aatcaataca aagcgcagac gcgtcacgtt
     5821 cagtcccagc gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag
     5881 aaaaaacagg ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa
     5941 ggtcacggtg gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg
     6001 atcggccagc gttttaatta gcgatatccc cgctccgcac aacgttccta acgcgatgcc
     6061 taccagcacc agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac
     6121 gcccgcgacc accatcagcc cgccacaaaa tgcgtacagc tggatgtaaa caatggataa
     6181 tccccacaga attgccgtgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc
     6241 cggtgaaact aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc
     6301 cgcgcccagt aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg
     6361 ttcctgcaca ggaacatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag
     6421 atgataagcc ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca
     6481 tccgtttacg attctcatta ggtcgccacc aatttttgat agtcggcgtc actgagtgaa
     6541 caatgccaga acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg
     6601 ggatccagcc aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggtggggca
     6661 tccagccagc caaagggcaa gccacttaaa aataagatgc gttgttctgc aacggctttc
     6721 acgccatgcc agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga
     6781 ataatatccg gctgccagcg caggagattt tccatggaaa cctgtgtcag gccttggcga
     6841 tcggctattt gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa
     6901 ccctttaagc ccgtctccag ccctcgaggg ccgcgcgcag cataaaagcg gaggttagcg
     6961 gcgggtgagg cggcgaatgc ctgagcttca ccaacgaagc ggctggcgag attggcttgt
     7021 tctgcggcgc gatgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat
     7081 tgcgccagtt tcccgttaag caataaccag ggtatttgtg tctgttcgct aacctgccgt
     7141 gcctgggaga tccaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac
     7201 gccataagcc cttccagcga gagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt
     7261 aactggcgaa tgtgctctga caatggaatt aatgcctgac gggcaaaatc aaaagaggaa
     7321 aaaccgacca ttttttctgg cgcgacagcc agcaatagca aatcggcggg cgcacctgcg
     7381 ctgacgatgc gtgtaatgtt ttgggcttcg ggaagcgtca gtgaggccca gaatttgact
     7441 gattgtgcaa aagaaggcag atgatgcagg aacaacgtcg acgcaagaat ctgtgtaaac
     7501 ctccgtctgg ttagtgacat aagttatatc ctgataatta aaaccgctat atatttacat
     7561 atatatcgaa aaaaatcatg tttgatgtca aaatttgtct aaagagacag aatgatttgc
     7621 ttgttgctta aataacactt tgttccacaa gaaaattgtc agggagaatg gc
//
LOCUS       DAZKFZ010000212         7611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000212 DAZKFZ010000000
VERSION     DAZKFZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(179..322)
                     /gene="ypaB"
                     /locus_tag="ACHOQW_004022"
     CDS             complement(179..322)
                     /gene="ypaB"
                     /locus_tag="ACHOQW_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP9575001.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            450..2735
                     /gene="nrdA"
                     /locus_tag="ACHOQW_004023"
     CDS             450..2735
                     /gene="nrdA"
                     /locus_tag="ACHOQW_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9575002.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2881..4011
                     /gene="nrdB"
                     /locus_tag="ACHOQW_004024"
     CDS             2881..4011
                     /gene="nrdB"
                     /locus_tag="ACHOQW_004024"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9575003.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4011..4265
                     /gene="yfaE"
                     /locus_tag="ACHOQW_004025"
     CDS             4011..4265
                     /gene="yfaE"
                     /locus_tag="ACHOQW_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP9575004.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4319..4969)
                     /gene="inaA"
                     /locus_tag="ACHOQW_004026"
     CDS             complement(4319..4969)
                     /gene="inaA"
                     /locus_tag="ACHOQW_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP9575005.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5050..6240)
                     /locus_tag="ACHOQW_004027"
     CDS             complement(5050..6240)
                     /locus_tag="ACHOQW_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9575006.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6392..7270
                     /locus_tag="ACHOQW_004028"
     CDS             6392..7270
                     /locus_tag="ACHOQW_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9575007.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT         2040 a         1973 c         1790 g         1808 t
ORIGIN      
        1 acaataaccg aatagaaaac aaccatttcg acatcaacaa tcgcttacat tcgcttatat
       61 attgaccaca actgatacat cagattatgt gatgactcgt gcttagatca atttttgcaa
      121 tcattagcaa aaagattaat aaaccatcta tatcaattta tccaacctat tctgccgttc
      181 aagaaatcgc cgaacagtta tttataacaa atttttctct tcccattgac tttcccggac
      241 accttgtctg acctaaggtg cgcgaaagcc actttttcct tcctgagtta tccacaaagt
      301 tatgcacttg caagagggcc attttcacac tatcttgcag tgaatcccaa acataccccc
      361 tatatatagt gttctaagca gcttcccgta ctacaggtag tctgcacgaa actattgcgg
      421 aaagaattcc aaaaacaggt acgacataca tgaatcagaa tctgctggtg acaaagcgcg
      481 acggtagcac agagcgcatc aatctcgaca aaatccatcg cgttctggat tgggcggcag
      541 aaggactgca taacgtttcg atttcccagg tcgagctgcg ctcccacatt cagttttatg
      601 acggtatcaa gacctctgac atccacgaaa ccattatcaa ggctgccgca gacctgatct
      661 cccgtgatgc gccggattat cagtatctcg ccgcgcgcct ggcgatcttc cacctgcgta
      721 aaaaagccta cggccagttt gagccgcctg cgctgtacga ccacgtggtg aaaatggtcg
      781 agatgggcaa atacgataat catctgctgg aagactacac ggaagaagag ttcaagcaga
      841 tggacacgtt tatcgatcac gaccgtgata tgaccttctc ttatgctgcc gttaagcaac
      901 tggaaggcaa atatctggta cagaaccgtg tgaccggcga aatctacgaa agcgcccagt
      961 tcctttatat tctggttgct gcgtgcctgt tctcgaacta cccgcgtgaa acgcgcctgc
     1021 aatatgtgaa gcgtttttac gacgcggttt ccacatttaa aatttcgctg ccgacgccaa
     1081 tcatgtccgg cgtgcgtacc ccgactcgtc agttcagctc ctgcgtactg atcgagtgcg
     1141 gcgacagcct ggattccatc aacgccacct ccagcgcgat tgtgaaatat gtttcccagc
     1201 gtgccgggat cggcatcaac gccgggcgta ttcgtgcgct gggtagcccg attcgcggtg
     1261 gcgaagcgtt ccataccggc tgcattccgt tctacaaaca tttccagact gcggtgaaat
     1321 cctgctctca gggcggcgtg cgcggcggtg cggcaacgct gttctacccg atgtggcatc
     1381 tggaagtgga aagcctgctg gtgttgaaaa acaaccgtgg tgtggaaggc aaccgcgtgc
     1441 gtcatatgga ctacggggta caaatcaaca aactgatgta tacccgcctg ctgaaaggtg
     1501 aagatatcac cctgttcagc ccgtccgacg taccggggct gtacgacgca ttcttcgccg
     1561 atcaggaaga gtttgaacgc ctgtatacca aatatgagaa agacgacagc atccgcaagc
     1621 agcgtgtgaa agccgttgag ctgttctcgc tgatgatgca ggaacgtgcg tctaccggtc
     1681 gtatctatat tcagaacgtt gaccactgca atacccatag cccgtttgat ccggccatcg
     1741 cgccagtgcg tcagtctaac ctgtgcctgg agatagccct gccgaccaaa ccgctgaacg
     1801 acgtcaacga cgagaacggt gaaatcgcgc tgtgtacgct gtctgctttc aacctgggcg
     1861 caattaatag cctggatgaa ctggaagagc tggcaattct ggcggttcgt gcacttgacg
     1921 cgctgctgga ttatcaggat tacccgatcc cggccgccaa acgtggagcg atgggtcgtc
     1981 gtacgctggg tattggtgtg atcaacttcg cttactacct ggcgaagcat ggtaaacgct
     2041 actccgacgg cagcgccaac aacctgacgc ataaaacctt cgaagcgatt cagtattacc
     2101 tgctgaaagc gtctaatgag ctggcgaaag agcaaggcgc gtgcccgtgg tttaacgaaa
     2161 ccacttacgc gaaagggatc ctgccgatcg atacctataa gaaagatctg gatgccatcg
     2221 ctaatgaacc gctgcattac gactgggaag ctctgcgtga gtcaatcaaa acacacggcc
     2281 tgcgtaactc cacgctttct gctctgatgc cgtccgagac ttcttcgcag atctctaacg
     2341 ccactaacgg tattgaaccg ccgcgcggtt acgtcagcat caaagcgtcg aaagacggta
     2401 ttctgcgcca ggtggtgccg gactacgagc atctgcacga cgcctatgag ctgctgtggg
     2461 aaatgccggg taacgatggc tacctgcaac tggtgggtat catgcagaaa tttatcgatc
     2521 agtcgatctc tgctaacacc aactacgatc cgtcacgctt cccgtcagga aaagtgccga
     2581 tgcagcagtt gctgaaagac ctgctcaccg cctacaaatt cggggtcaaa acgctgtatt
     2641 atcagaacac ccgtgacggc gctgaagacg cgcaggacga tctggtgccg tcaatccagg
     2701 acgatggctg cgaaagcggc gcatgtaaga tctgatattg agatgccgga tgcggcgtaa
     2761 acgccttatc cggcctacgg ctcggtttgt aggcctgata agacgcgcca gcgtcgcatc
     2821 aggcacagga tgcggcgtaa aatgccttat ccggcattaa actcccaaca ggacacactc
     2881 atggcatata ccaccttttc acagacgaaa aatgatcagc tcaaagaacc gatgttcttt
     2941 ggtcagccgg tcaacgtggc ccgttacgat cagcaaaaat atgacatctt cgaaaagctg
     3001 atcgaaaagc agctctcttt cttctggcgt ccggaagaag ttgacgtttc tcgtgaccgt
     3061 atcgattacc aggcgctgcc tgaacacgaa aaacacatct ttatcagcaa cctgaaatat
     3121 cagacgctgc tggactccat tcagggtcgt agcccgaacg tggcactgct gccgctgatc
     3181 tcgatcccgg aactggaaac ctgggtagaa acctgggcgt tctcagaaac gattcattcc
     3241 cgttcctata ctcatatcat tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat
     3301 atcgtcacca acgagcagat ccagaaacgt gcggaaggga tctccagcta ctacgatgag
     3361 ctgatcgaaa tgaccagcta ctggcacctg ctgggcgaag gcacccacac tgttaacggt
     3421 aaaaccgtga ccgttagcct gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc
     3481 gttaacgcgc tggaagcgat ccgcttctac gtcagctttg cttgttcctt tgcctttgca
     3541 gaacgcgaac tgatggaagg taacgccaaa attattcgcc tgattgcccg cgacgaagcc
     3601 ctgcacctga ccggtactca gcatatgctg aatctgctgc gcagcggtgc ggatgatccg
     3661 gagatggcgg aaattgccga agaatgtaag caggagtgct atgacctgtt tgtccaggcg
     3721 gcacagcaag agaaagactg ggcggattat ctgttccgtg acggttcgat gattggtctg
     3781 aataaagaca ttctctgcca gtacgttgaa tacatcacca atatccgcat gcaggcagtc
     3841 ggtttggatc tgccgttcca gacgcgctcc aacccgatcc cgtggatcaa cacctggctg
     3901 gtgtctgata acgtgcaggt tgctccgcag gaagtggaag tcagttctta tctggtcggg
     3961 cagattgact cggaagtgga caccgacgat ttgagtaact tccagctctg atggctcgcg
     4021 ttaccctgcg catcactggc acacaactgc tgtgccagga tgaacaccct tcccttctgg
     4081 cggcgctgga atcccacaat gtggcggttg agtaccagtg ccgcgaaggt tactgcggct
     4141 cctgtcgcac acgcctggtt gcaggccagg tggactggat tgccgaaccg ttagccttta
     4201 ttcagccggg ggaaattttg ccctgttgtt gccgagcaaa aggcgatatt gaaatcgagt
     4261 tgtgatcggg tactagcgcc agatacgacg cttacgcgta ttatctggcc tacaacgatt
     4321 acatggcgta gtaatacgct tttacctgct cccagtccgc tttggggatc ggctccagat
     4381 atttttccag ctggcggaag tcatgattaa tcgctttatc gcgacgcaag cggcgacgac
     4441 ttttctccag atcaagaaaa ccagcttctg ccttaccttc tgttttcaca tagatatggc
     4501 gaacataaca acagccatgc tgacgattaa cgctatgcat tttcttaaac gccagcgcca
     4561 ctgctttcaa catggcttgc cgtacttcgt cagaataggg cgttactgca tgctgggcat
     4621 accagtcagc aatgctgatg aaccccgcca tatcttcagt caccagcaac gcgcgccatt
     4681 ccccctcaat tttcaccgct tcaccaaaaa cgatcttcgg cacgatgaca ccggcccgtt
     4741 ccagttcttt aattaccgca acctcacgga caatcgttgg tcggccgaaa ggataacgta
     4801 cggaatgaaa cagatgatgc gtcatgcgct ttacatatag ctttttgccg ttgcgctcga
     4861 cgcattgcac cccgctcata ccattacggc gatagttagg ctcttcaacc cagtcgccct
     4921 ctgttgccca ccagtgatta aattcgtcgt gctttgctga aactgccata ccctatcgcc
     4981 tgtcattttt tattaacgac aatgactata ggtggttacc tgcgaaaaat cttaatgaaa
     5041 cgtgtcgtat taatgaattt cttttgccac tactagcgcg acaccgcttt ttcccttcat
     5101 ttgcagcgca tagactccac cagccactac cactaatagt gcgcccagca gataaatcat
     5161 tcggtagcca gtatgtgcaa ccatcatccc catcacacag gctcccagcg ccaggcctgc
     5221 atccatcgca ttgaagaaga gggagtttgc gacaccgatt ttatgcggtt ccactgaact
     5281 gataatctgc gtctgaaaaa ctggcgtcac ggaaccatac ccgataccaa tcaacgcgcc
     5341 agaaacgacc atcattacgc tgccctgggt gtagccgaga accaccagcc caaccgtaaa
     5401 gaacagcagg caggggtaaa ccacatattt cgggcctttt ttgtcgcaaa cattgccggt
     5461 gaaggtacgg cagatcatca ggaagatggc gtagcagagc aggaaattac tggccgccga
     5521 catcagatta agttcgcggg cgtaaagcgc cagaaaggca gaaaccccgg catacgaaaa
     5581 ggtcataaag aatgtaacca tggcaaacgg caatgccgcg cgatcgaaca tggcggcgaa
     5641 gcccatcttt ggctttgaac cgtcggcatg gctgataacc ggcggcacgg tcataatcag
     5701 cgacaggata atccccaccg ccgcaacgcc agtacataac cagaaagcca tattaaaggc
     5761 ctcccagcgc gccatattca ggccaatcca cggcccgacc accattgcca gccccattgc
     5821 cagagagaaa aaactgatgc cctccccacg tcgggaggcc gggatcagcc gcgcggaaat
     5881 cgttcccttt accgttgtga taaccccgaa ggtaatacca tgaagcaccc taataaaaag
     5941 cagtgattct attgagtggc aaaaaggata cagcgcagtg accaccaaaa aggccagaga
     6001 agagagcacc agaatagttt tattcgaata tttacccacc cattgcccgg caaagggtcg
     6061 aataacaatc gccgcaatta aaaataaagt caccaacaaa cctgctttat caggagaggc
     6121 atgcaagcgg tcagagatat aaattggcaa caatgttagt aaaacataaa agacgaagaa
     6181 aataataaag ctgatgatgg ttattgccca aaaatccttc gtccataact tctctttcat
     6241 actctaccgc ctgtcaggat gacgcacgca gacaacactc ccgttactgc ggttgcgtca
     6301 aattgtgatg tactttgcag aacagaagta taaatggcgg catgctatta gagaaattaa
     6361 tcgttttaat attattgatt agcaaactct aatgactctt acccaaataa attctttact
     6421 cgccgtgctg gattacggtg gctttactga agccagcaag cgattgttca tgacgcagtc
     6481 ggcggtaagt caggcaattg ctgccctcga acaagaactt ggtgtgaata tactgttacg
     6541 cgatcgccgt aaagctatac aactgaccac agcaggtcac cgcattgtgc aacatctgcg
     6601 cgccattaat cgggaagtta acgcagtaaa agagatagcc gagcaggaaa agcagaaccc
     6661 tcaaagaacg ctgagacttg gttgtttccc cagcgtctgc gcctgtatct taccggttgt
     6721 gattcgctac tttgaaattc atcatccgaa tataaaaatt attccttttg aggaaaatag
     6781 cacagcgatt attgattcgc ttcagaatga atccattgat gccggttttg ttcattttcc
     6841 ggtgagcgga atgtactccg tgcccattta tcaggataaa ttcaccgtag tgttaccgcc
     6901 caaccatgct ctggcaaaaa acagcactat tacagtggaa gaattaatgg gtgaaccatt
     6961 aatcattagt aaaggacgct atgaattaag cattatggca ctatttaaag aaaagaatat
     7021 taccccacag ataaaatatg aatttaatca cccggataca gcgatcagtt ttattcgaca
     7081 gggattaggc atcgccttat taccagagtt aacgctgaaa accattgcag atgaattgtg
     7141 ttccgttcct ctcgaaccaa ccttctatcg acagatttcg ttgctggcta aagaaaagcc
     7201 ggtagaaggc agtccactgt ttttactaca aatgtgtacg gaacaactag tggtgagtgg
     7261 aaaaatttga tgtaagacat gagattgatg acaaatgcaa gactgcctga ttcgccacac
     7321 ttagttgtac tatagccttt accaggcgta ataattcgtt ttcacctgat tttggcccac
     7381 tttgtgaatt ggcgatatat ttatccagtt gggagaagtg acaatgactc caggtagaaa
     7441 ccggtatgaa gcgcccgact cactgtcaaa accgattgtt gagagtaatg acactccggc
     7501 gaacagtaca cagtttactc tggagccatt ctcaatcttc aagtgggata cctaataact
     7561 tgtcagggaa gttatcaata tctgtttgtt acttattacc gtatttatta a
//
LOCUS       DAZKFZ010000213         7519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000213 DAZKFZ010000000
VERSION     DAZKFZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            82..177
                     /gene="ymiC"
                     /locus_tag="ACHOQW_004029"
     CDS             82..177
                     /gene="ymiC"
                     /locus_tag="ACHOQW_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP9575008.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            341..3016
                     /gene="acnA"
                     /locus_tag="ACHOQW_004030"
     CDS             341..3016
                     /gene="acnA"
                     /locus_tag="ACHOQW_004030"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP9575009.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3080..3670)
                     /gene="ribA"
                     /locus_tag="ACHOQW_004031"
     CDS             complement(3080..3670)
                     /gene="ribA"
                     /locus_tag="ACHOQW_004031"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP9575010.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            3840..4604
                     /gene="pgpB"
                     /locus_tag="ACHOQW_004032"
     CDS             3840..4604
                     /gene="pgpB"
                     /locus_tag="ACHOQW_004032"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP9575011.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            4753..5061
                     /gene="lapA"
                     /locus_tag="ACHOQW_004033"
     CDS             4753..5061
                     /gene="lapA"
                     /locus_tag="ACHOQW_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP9575012.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            5068..6237
                     /gene="lapB"
                     /locus_tag="ACHOQW_004034"
     CDS             5068..6237
                     /gene="lapB"
                     /locus_tag="ACHOQW_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP9575013.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6430..7167
                     /gene="pyrF"
                     /locus_tag="ACHOQW_004035"
     CDS             6430..7167
                     /gene="pyrF"
                     /locus_tag="ACHOQW_004035"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9575014.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7167..7493
                     /gene="yciH"
                     /locus_tag="ACHOQW_004036"
     CDS             7167..7493
                     /gene="yciH"
                     /locus_tag="ACHOQW_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP9575015.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         1825 a         1777 c         2042 g         1875 t
ORIGIN      
        1 attaaagggc ttttaataca ccgcagcaat aacaggttga gttatctcaa cacaaagtaa
       61 taaccgttaa gggtgtagcc tatgatcaac acaaatatga aatattggtc ctggatgggt
      121 gcattttctc tgtcgatgct cttctgggcc gaactcctct ggatcattac tcactgatcc
      181 ttgaccccgc tgcggcgggg ttgtcatttg ctttgccaca aggtttctcc tcttttatca
      241 atttgggttg ttatcaaatc gttacgcgat gtttgtgtta tctttaatat tcaccctgaa
      301 gagaatcagg gcttcgcaac cctgtcatta aggaggagct atgtcgtcaa ccctacgaga
      361 agccagtaag gacgcgttgc aggccaaaga taaaacttac cactactaca gcctgccgct
      421 tgctgccaaa tcactgggcg atatcacccg tctacccaag tcactcaaag ttttgctcga
      481 aaatctgctg cgctggcagg atggtaactc ggttaccgaa gaggatatcc acgcgctggc
      541 gggatggctg aaaaatgccc atgctgaccg tgaaattgcc taccgcccgg caagggtgct
      601 gatgcaggac tttaccggcg tacctgccgt tgttgatctg gcggcaatgc gcgaagcggt
      661 taaacgcctc ggtggcgata ctgcaaaggt taacccgctc tcaccggtcg acctggtcat
      721 tgaccactcg gtgaccgtcg atcgttttgg tgatgatgag gcatttgaag aaaacgtacg
      781 cctggaaatg gagcgcaacc acgaacgtta tgtgttcctg aaatggggaa agcaagcgtt
      841 cagtcggttt agcgtcgtgc cgccaggcac aggcatttgc catcaggtta acctcgaata
      901 tctcggcaaa gcagtgtgga gcgaattgca ggacggtgaa tggattgctt atccggatac
      961 actcgttggt actgactcgc acaccactat gatcaacggc cttggcgtgc tggggtgggg
     1021 cgtgggtggc atcgaagcag aagccgcaat gttaggccag ccggtttcca tgcttatccc
     1081 ggacgtagtg ggctttaaac ttaccggaaa attacgtgaa ggtattaccg ccacagacct
     1141 ggttctcact gttacccaaa tgctgcgcaa acatggcgtg gtggggaaat tcgtcgaatt
     1201 ttatggtgat ggtctggatt cactaccgtt ggcggatcgc gccaccattg ccaatatgtc
     1261 gccagaatat ggtgccacct gtggcttctt cccaatcgat gctgtaaccc tcgattacat
     1321 gcgtttaagc gggcgcagcg aagatcaggt cgagttggtc gaaaaatatg ccaaagcgca
     1381 gggcatgtgg cgtaacccgg gcgatgaacc gatttttacc agtacgttag atctggatat
     1441 gaatgacgtt gaagcgagcc tggcagggcc taaacgccca caggatcgcg ttgcactgcc
     1501 cgatgtacca aaagcatttg ccgccagtaa cgaactggaa gtgaatgcca cgcataaaga
     1561 tcgccagccg gtcgattatg ttatgaacgg acatcagtat cagttacctg atggcgctgt
     1621 ggtcattgct gcgataacct cgtgcaccaa cacttctaac ccaagtgtgc tgatggccgc
     1681 aggcttgctg gcgaaaaaag ccgtaactct tggcctcaag cggcaaccgt gggtcaaagc
     1741 gtcgctggca ccgggttcga aagtcgtttc tgactatctg gcaaaagcga aactgacacc
     1801 gtatctcgac gaactggggt ttaaccttgt gggatacggt tgtaccacct gtattggtaa
     1861 ctctgggccg ctgcccgatc ctatcgaaac ggcaatcaaa aaaggcgatt taaccgtcgg
     1921 tgcagtgctg tccggcaacc gtaactttga aggccgtatc catccgctgg ttaaaactaa
     1981 ctggctggcc tcgccgccgc tggtggttgc ctatgcgctg gcgggaaata tgaatatcaa
     2041 cctggcttct gagcctattg gtcatgatcg taaaggcgat ccaatatatc tgaaagatat
     2101 ctggccatcg gcacaagaaa ttgcccgtgc ggtagaacaa gtctccacag aaatgttccg
     2161 caaagagtac gcagaagtct ttgaaggcac tgcagagtgg aaagaaatta acgtcgcacg
     2221 atccgatacc tacggatggc aggaggattc aacctatatt cgtttatcgc ctttctttga
     2281 tgaaatgcag gcaacgccag cacctgtgga agatattcac ggtgcgcgga tcctcgcaat
     2341 gctgggggat tcagtcacca ctgaccatat ctctccggcg ggcagtatta agcccgacag
     2401 cccagcgggt cgatacctac aaggtcgggg tgttgagcga aaagacttta actcctacgg
     2461 ttcgcggcgt ggtaaccatg aagtgatgat gcgcggcacc ttcgccaata ttcgcatccg
     2521 taatgaaatg gtgcctggcg ttgaaggggg gatgacgcgg catttacctg acagcgacgt
     2581 agtctctatt tatgatgctg cgatgcgcta taagcaggag caaacgccgc tggcggtgat
     2641 tgccgggaaa gagtatggat caggctccag ccgtgactgg gcggcaaaag gtccgcgtct
     2701 gcttggtatt cgtgtggtga ttgccgaatc gtttgaacga attcaccgtt cgaatttaat
     2761 tggcatgggc atcctgccgc tggaatttcc gcaaggcgta acgcgtaaaa cgttagggct
     2821 aaccggggaa gagaagattg atattggcga tctgcaaaat ctacaacccg gcgcgacggt
     2881 tccggtgacg cttacgcgcg cggatggtag ccaggaagtc gtaccctgcc gttgtcgtat
     2941 cgacaccgcg acggagttga cttactacca gaacgacggc attttgcatt atgtcattcg
     3001 taatatgttg aagtaacaag tatttgcttg ccggttattt tgcttccggc aagcaaatta
     3061 attacacaat gcaagagggt tatttgttca gcaaatggcc cattttctcg gctttggtat
     3121 cgagataatg ttcgttattg gggttacgac ctacaatcaa tggtacgcgt tcaacaatat
     3181 taatccccgc ttcggtcaga atttcgactt ttttcgggtt attggttaac aagcggactt
     3241 cattgacgcc aaggagtttg aacatatcag cgcaaagagt gaagtcgcgc tcatcagcgg
     3301 cgaagcctaa ctggtggtta gcctctacag tatcgtaacc ttgatcctgc agtgcgtaag
     3361 cgcggatttt attcagcaga ccaatgttac gaccttcctg acggtgatac agcaaaatac
     3421 cacggccttc ctcggcaatt tgcgtcaatg ccgcttcgag ctggaagcca caatcgcagc
     3481 gcaagctgaa cagggcgtca ccggtcagac attcggaatg gacgcgcgca agtaccgggg
     3541 tatgcccgga aatatcgcca tagactagcg cgacatgatc gtgtccggtt gccagttctt
     3601 caaatcccac catcaggaaa tcgccccatg gggttggcag tttggcttct gccacacgtt
     3661 taagctgcat gaaattctcc agataatgct ggttctgtat tggcttattt tgccataacg
     3721 agaaggggtt cacctaatca cgacgcgtcg atcgttcacg gaatggcaca aatctgtcaa
     3781 tttttcctgg aactggcatt ttcagttatg attgtgggac ttatcaaaaa ggagaggcca
     3841 tgcgttcgat tgcaagacgt accgcagtgg gagctgcact attgcttgtt atgccagtag
     3901 ccgtatggat ttctggctgg cgttggcaac ctggagaaca aagttggcta ctaaaagcgg
     3961 ctttttgggt tactgaaact gtcacccagc cctggggcgt cattacacat ttgattttat
     4021 tcggctggtt tctctggtgt ctgcgttttc gcattaaggc tgcctttgta ttatttgcca
     4081 ttctggcggc cgcaatcctt gtgggacaag gcgttaaatc ctggatcaaa gacaaagtcc
     4141 aggaaccacg accttttgtt atctggctgg aaaaaacaca tcatattccg gttgatgagt
     4201 tctacacttt aaagcgagca gaacgcggaa atctagtgaa agaacagttg gctgaagaga
     4261 aaaatattcc acaatatttg cgttcacact ggcagaaaga gacggggttt gcctttcctt
     4321 ccggtcacac gatgtttgct gccagttggg cactgctggc cgttggtttg ctgtggccgc
     4381 gtcggcgaac gttaaccatt gctatcttgc tggtctgggc aacgggagtc atgggaagcc
     4441 gcctgctgct cgggatgcat tggccacgcg atctggtggt agctacgttg atttcgtggg
     4501 cactggtggc ggtggcaacg tggcttgcgc aacgaatttg cgggccatta acaccacctg
     4561 cggaagaaaa tcgcgaaata gcgcaacgag aacaagaaag ttaacgctgg ttgattttcc
     4621 gaatttagcc cttaaatcat caacaatgcg cgtggatgcc atttcgcaga cggcgcgaaa
     4681 atggtacttt aaagggctat tgcggtaagt tgaccataat ttattcgctc taaccacata
     4741 acgggaagta atgtgaaata tttactcatt ttcttactgg tgttagcgat cttcgtgatt
     4801 tcggtcacgt tgggtgcgca gaacgatcaa caggtgacgt ttaattatct gttagcgcaa
     4861 ggggagtacc gtatttccac attgctggcg gtattgtttg ctgcgggttt tgctatcggt
     4921 tggttgattt gtggcctgtt ctggctgcga gttcgtgttt ccctggtgcg cgctgaacgt
     4981 aaaataaagc gactggaaaa ccagctttca cccgcgactg acgtggctgt agtgcagcac
     5041 tcgtcagcgg cgaaggaata actttctatg ctggagttgt tgtttctgct tttgcctgta
     5101 gccgctgcct atggctggta tatgggccgc agaagtgcgc aacaaaacaa gcaagatgaa
     5161 gccaaccgct tgtcgcgtga ttacgtagcg ggggttaact tcctgcttag taatcaacag
     5221 gataaagcgg tagacctgtt tctcgatatg cttaaagagg atacaggcac cgttgaagcc
     5281 caccttacgc tcggaaacct gttccgttcg cgtggcgaag ttgatcgcgc tattcgcatc
     5341 catcagaccc taatggaaag cgcctcgctg acctatgaac agcgtctgtt ggcgattcaa
     5401 caactggggc gtgattacat ggccgccggg ttatatgacc gcgcggaaga catgttcaat
     5461 cagctgaccg atgaaactga cttccgcatt ggcgcgctgc aacagttgct acaaatctac
     5521 caggctacca gtgagtggca gaaagcaatt gatgttgccg aacgcctggt gaagctgggt
     5581 aaagataaac agcgcgtcga aattgcccat ttctactgtg agttagccct gcagcatatg
     5641 gccagcgacg atctcgatcg tgccatgacc ttgctaaaaa aaggggcggc ggcagataaa
     5701 aacagcgccc gcgtatccat catgatggga cgcgtgttta tggcgaaagg agaatacgcc
     5761 aaagccgtcg aaagtttgca acgcgtgata tcccaggaca gagaactggt cagcgaaacg
     5821 ctggaaatgc tgcaaacctg ctatcagcag ttgggtaaaa ctgccgaatg ggcagaattc
     5881 ctgcagcgcg cggtggaaga gaacaccggt gccgatgctg aattgatgct tgctgatatc
     5941 atcgaagcgc gcgacggtag tgaggccgca caggtctata ttacgcgcca gcttcagcgt
     6001 catccgacca tgcgtgtgtt ccataagtta atggattacc acttaaatga agcggaagaa
     6061 gggcgtgcca aagagagcct gatggtgctg cgtgacatgg ttggcgagaa ggtacgtagt
     6121 aagcctcgtt atcgctgcca gaaatgtggt tttaccgcat acaccctcta ctggcattgt
     6181 ccgtcttgtc gtgcctggtc aactattaaa ccgattcgcg gtcttgatgg cctgtaattt
     6241 tttaaaaaaa ccgactttag ttacaacata ctagttattt atgtcccatt gtgctccggc
     6301 aacggcggcg cagaaaaagc ctgccagggg agaaatcgca actgttaatt ttttatttcc
     6361 acgggtagaa tgctcgccgt ttacctgttt cgcgccactt ccggtgccca tcatcaagaa
     6421 ggtctggtca tgacgttaac tgcttcatct tcttcccgcg ctgttacgaa ttctcctgtg
     6481 gttgttgccc ttgattatca taatcgtgat gccgcgctgg cctttgtcga caagatcgac
     6541 ccacgcgatt gtcgtctgaa ggtcggcaaa gagatgttta cattgtttgg gccacagttt
     6601 gtgcgcgaac ttcaacagcg tggttttgat atctttcttg acctgaaatt ccacgatatc
     6661 cccaacactg cagcgcacgc tgtcgctgct gcagctgact taggcgtgtg gatggtgaat
     6721 gttcatgcct ctggtggggc gcgtatgatg accgcagcgc gtgaggcact ggttccgttt
     6781 ggcaaagatg caccgctttt gattgctgtg acagtgttga ccagcatgga agccagcgac
     6841 ctggccgatc ttggcgtgac actgtcacct gcagattatg cagaacgtct ggcagcactg
     6901 acgcaaaaat gtggccttga tggtgtggtg tgttctgctc aggaagctgt gcgctttaaa
     6961 caggtattcg gtcaggagtt caaactggtt acgccgggca ttcgtccgca ggggagtgaa
     7021 gctggtgacc agcgccgcat tatgacgcca gaacaggcgt tggcggctgg tgttgattat
     7081 atggtgattg gtcgcccggt aacgcaatcg gtagatccag cgcagacgct gaaagcgatc
     7141 aacgcctctt tacagcggag tgcatgatga gtgattccaa cagccgtctg gtctactcaa
     7201 cagaaaccgg gcgtattgat gagcccaaag cggccccagt acgctctaaa ggcgatggtg
     7261 tggtgcgtat tcagcgtcag accagtggac gtaaaggtaa gggcgtttgc ctgattaccg
     7321 gtatcgatct cgatgatgcc gaactgacaa aacttgcagc ggaactgaag aaaaaatgcg
     7381 gctgcggcgg agcagttaaa gatggagtta ttgaaatcca gggcgataag cgtgatttat
     7441 taaaatcact acttgaagcg aaagggatga aggtaaaact cgcaggcggt taacataaaa
     7501 agccacggat atatccgtg
//
LOCUS       DAZKFZ010000214         7451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000214 DAZKFZ010000000
VERSION     DAZKFZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..690)
                     /locus_tag="ACHOQW_004037"
     CDS             complement(<1..690)
                     /locus_tag="ACHOQW_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9575016.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRR"
     gene            complement(701..1702)
                     /gene="ltaE"
                     /locus_tag="ACHOQW_004038"
     CDS             complement(701..1702)
                     /gene="ltaE"
                     /locus_tag="ACHOQW_004038"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP9575017.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1739..3457)
                     /gene="poxB"
                     /locus_tag="ACHOQW_004039"
     CDS             complement(1739..3457)
                     /gene="poxB"
                     /locus_tag="ACHOQW_004039"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP9575018.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3590..4558)
                     /gene="hcr"
                     /locus_tag="ACHOQW_004040"
     CDS             complement(3590..4558)
                     /gene="hcr"
                     /locus_tag="ACHOQW_004040"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP9575019.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4570..6222)
                     /gene="hcp"
                     /locus_tag="ACHOQW_004041"
                     /gene_synonym="priS"
     CDS             complement(4570..6222)
                     /gene="hcp"
                     /locus_tag="ACHOQW_004041"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP9575020.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6366..7265)
                     /gene="lysO"
                     /locus_tag="ACHOQW_004042"
     CDS             complement(6366..7265)
                     /gene="lysO"
                     /locus_tag="ACHOQW_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP9575021.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1791 a         2017 c         1969 g         1674 t
ORIGIN      
        1 gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac tgagcacctc
       61 tggtccggcg gcttcgaaga tgcggtgttc gttggctgga tggtctaaca gcgccaccag
      121 atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc agcgtggcgg
      181 cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgctg ctgaacctgc
      241 accaacgatt attccggcac gcaattcggt cactggtaca ttcgcttcac gaagaatgtc
      301 cgccgtagcc tgacgcgcgc gcagatggtc cgactgctca tgtggcgggg cctgcaacga
      361 actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga agttgagagc
      421 cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctatgca ccagaaaata
      481 gaccgtatcg atatcctgta acagggccgg aaggttatcc ggccagctga gatcgacttt
      541 atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac gtgccgccgc
      601 caggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa tgtagccact
      661 ggcaccaaga actaaaatgc gttgcggcac gtctccctcc ttaacgcgcc aggaatgcgc
      721 gccagtgggc ggcgacttct gccagttgtt cgcgcgagac gtcaagatgg gtcaccaggc
      781 ggacaatcgg cgaggcgtta atcagtacgt ttctcgcttt catgtattcg cctaacgcgg
      841 cagcattttc ttcgccgacg cgaacaaaca gcatattagt gtcctgacgc atcacatccg
      901 cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct tcctgcaacc
      961 gtgcgacgtt atttttcagc gcatacatcc cggcggcagc cagaatgccg gactggcgca
     1021 tcccgccacc tgtcattttc cgccagcgaa tggcacgttt aatgtaatca cgattaccga
     1081 cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg aacgaatcac
     1141 aatattgcgt gagctctttc agttcgctgc cgtaagccac cacggcatta aagatgcgcg
     1201 caccgtcaac atgcagcgcc agattgcgct cgcgggtaaa ttcccatgca tctttaaggt
     1261 attcacgcgg caacactttg ccgttgtggg tgttttccag actgagtaat ttggtgcggg
     1321 cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt agcgtgccgt
     1381 cggcagccgc gtcgatgggt tgcggttgaa tactgcccag caccgccgcg ccaccggctt
     1441 caaacagata gttatgcgcg gcctgaccga caatatactc ttcgccgcgt tcgcagtgac
     1501 tgagcagagc gaccaggttg gcctgagtgc cggtaggcag aaaaatggcg gcttctttac
     1561 cggaaagctc tgctgcatag tcctgcagag cattaacggt agggtcgtct ccgtaaacgt
     1621 cgtccccaac cggggcggcc atcatcgctt cgagcatggc gcggctcggt cgggttacgg
     1681 tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc cacccttttt
     1741 accttagcca gttcgttttc gccagttcga tcacttcatc accgcgtccg ctgatgattg
     1801 cgcgcagcat atacaggctg aaacctttgg cctgttcgag tttgatctgc ggtggaatgg
     1861 ctaactcttc tttggcgacc accacatcca ccaataccgg accgtcgatg gagaaggcgc
     1921 gttgcagggc ttcatcgatt tcagaggctt tttctacacg aatacccgta attccgcacg
     1981 cttcggcaat gcgggcaaag ttggtgtcgt gcagctcagt accgtctgtc aggtagcctc
     2041 cggctttcat ctccatcgcc acaaagccca gcacgctgtt attaaagacg ataattttca
     2101 ccggcagttt catctgcact accgagagga aatcgcccat caacatgcta aaaccgccat
     2161 cgccgcacat ggcgaccacc tgacgttccg gctcggttgc ctgcgcgccc agcgcctgcg
     2221 gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc ttgccgttca
     2281 ttttcagata acgcgccgcc cacaccgttg gcgtgccgac gtcacaggta aagatggcgt
     2341 catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg gctttctcgc
     2401 tcggtttagc taaatcgtcc agccctttgc gggcatcgcg gtaatcttcc agcgctttat
     2461 ccagaaactt gcgatcggct ttttcttcca ccaatggcag caatgcacgc agggtcgact
     2521 tgatatcgcc gaccagcgcc atatccacct tgctgtgtgc gccgatgctg gctgggttga
     2581 tatcaatctg aataattttg gcatcggtcg ggtagaacgc acggtagggg aactgagtac
     2641 cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag aatccgatta
     2701 acccggtcat tccaacatca tacggattat cgtattcgac atgctcttta ccgcgtaggg
     2761 catgaacaat aggtgcttta attttcccgg caaactcaac taactcttta tgcgcccccg
     2821 cgcagccgct gccacacatc agagcgatat tgctggaata acgcagcagt tgcgccagtt
     2881 tgcgtaactc ttcttcttcc ggcgtcacga ctggctgtgg cgcatgatac cagtgggtag
     2941 ttgccccttc tggcgcaggt tttaacgcca cgtcgcccgg taacacgaca acggaaacgc
     3001 cacggttaag caccgctttg cgcatggcaa tcgccagcac ttgtgggatc tgctccgggc
     3061 tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg tgggtttcct
     3121 ggaaatagcc gctgccaatt tcgctggaag gaatatgagc ggcaatcgcc agtaccggaa
     3181 cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg gggccgcacg
     3241 atccagcaca gaccgccagt tctcctgaaa gttgtgcttc agcgccagcg gcaaacgccg
     3301 ccacttcttc atggcgggtg gacatccact cgatggtgcc catgcgatta agactgtcgc
     3361 taagaccgtt cagagagtcg cccgtgactc cccagatgcg tttcacgcct gccgattcga
     3421 gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc ctgaatgtga
     3481 taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag aaaggaggca
     3541 ctaacggtta aatagcccga tgaaaggaat atcatcgggc ataaggcgat tatgcgagaa
     3601 ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg atttcggcgt
     3661 cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttc gtcttacagc
     3721 agccgcaaac acccgcgcgg caggcggcga caaccggaac gttattgctt tccagcgcct
     3781 ccagcagcgt ggtgccaaca ggagcgtaaa attcttgcgc cggttgcagt ttggtgaatt
     3841 tcagaccgct ggtcgccgct tccgctactg gggtgaagaa tttctcttta aagaaacgcg
     3901 tcacgccgag tgctttcact tcctgctcta cccaatccat atacggagcc gggccgcagg
     3961 tcatcacggt acgtgaagct aagtcaggaa cgcgagtcag gagttcgcga gtgagacgac
     4021 cagcgataaa gccttcggta acgttatttt ccgccaccag cgttaccgga tagttacgcc
     4081 actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc cgcacatcgg
     4141 cctgtggacg gttcttcgcc agccagcgac gcatcgacat aatcggcgtg acgccgcagc
     4201 ctgcagccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat tcccccatcg
     4261 cgtctgaaag ccagagataa tcaccgcgtt ttacatcgcg ggtcagccac tgggagccga
     4321 caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct ggcgtggagg
     4381 aaatggtgta agcacgcagc gtttccgctg agttacgcac gctgaccagt gcatattgcc
     4441 cggcgcggta tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca tccggcgttt
     4501 cttgcttaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc atcgtcatcg
     4561 acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg tgatagaacg
     4621 caggccgaat ttctcgttca gcaccgccag cagatctggt gtcaggaaac caggtgcagt
     4681 cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa tgacaatcgc
     4741 tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttgacac cgcagcccag
     4801 tttttctgcc agcgtaaccg ccagaataat tgctgagtaa gcatcgttac actgaccggc
     4861 atccaccaga cgaggcagac cttcgatatc gccaaactca agtttgttaa agcgatattt
     4921 accacaggcg agagtcagga tcaggcagtc atctggcacg ctggtggcga aatcggtgaa
     4981 gtagtgacgt tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat gacgcagttt
     5041 ttcacggctc accagatcaa ttagcgtatc cgctgcgcca agcagcgtct ggcggccaaa
     5101 gcccacggtg atcaggtgcg gaatttcgct gtatgggaag cctgccattt gttgcgcctg
     5161 ggcaatgacc gcagagaaat cttcgccatc cagatgacgc acgccaggcc agccaacgat
     5221 gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga tgcagttcga
     5281 ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct gccagccgct
     5341 gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat gcgcaggcag
     5401 catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca gcaggttgta
     5461 gagatctttg agatcgtgac cggaaatcag gatgcattta cccgccgtcg ctttgacgtt
     5521 gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca gaatgctcat
     5581 cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt tcatatcggc
     5641 aggccaggtc cccagccacg ccatgatttt atgatactgg gcataaatat cgttgtcgta
     5701 ttgaccgaga acgtgtgcgt gttccatata ggccgccgca cctttcaggc catacaggca
     5761 cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt taggggtaaa
     5821 ttctgctgcc tgacgttgca gttcgccgag atcgtcgctc accagttgca ggtcagccat
     5881 cgggttatcg acgcgggcgt tggcatctac agccaggcat tgtgctttca gcgcctcgcg
     5941 caaggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt taacgttggt
     6001 cagggttgag aaaaaggcac gtggcgcgaa gctgtcgaca tcgtggttga tgatgccgta
     6061 ttcacgcgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga gtaaatcctg
     6121 aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc agccgtttcc
     6181 tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac cttttaaagt
     6241 tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa gggatttttc
     6301 atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggatct accgccagag
     6361 aggtattacg cggagaaaaa ggcgatgagg atcggcacta acaggctaag aataaaaccg
     6421 tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac gggcagggtg
     6481 aaatccatcg acgtcgcacc gcataagccc agtgcggtag agcggctgcg gcgaatcagc
     6541 ccagggatca acataattgc aatcagctca cgggccagat cattaaaaaa cgccgcgctc
     6601 ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata ccagccgaaa
     6661 ccagaggcca ttgccagcgc ggtattgatg ggtagatcaa gaataaaggc gttaattaaa
     6721 ccaccaatta atgaactggc aaccaccacc acggcgacaa tcattccccg gcgattaagg
     6781 acaatctgct ttaaggtcat gccattattg cgcaactgaa taccgacgag gaaaagtagc
     6841 agaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag tccactcagg
     6901 ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactcaag cgccatcgca
     6961 atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcagacc gcgctccagc
     7021 cacataaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc agaataatgc
     7081 agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat acccataaaa
     7141 aagagaataa ggtaaaccat ccagcttaat agctgattaa taacttttaa cgcagcttgt
     7201 tggcgaagcg gaatgaggta acccacaatc aggggaacca gaatgattaa caacccagaa
     7261 aacatgaaaa accagccctt gcaaagaaag aagtcgaaat gcgcgatgac acactactga
     7321 aagcggaagg acgagtaaag ttgcaattaa aaggaaatgt tatgcatcag agggggattt
     7381 ttaattctat atgagtgttt agcattgttg cgctcctttc atgtgcaatg ccatataaag
     7441 ccactggcgc a
//
LOCUS       DAZKFZ010000215         7407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000215 DAZKFZ010000000
VERSION     DAZKFZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.215"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1704
                     /gene="adeD"
                     /locus_tag="ACHOQW_004043"
     CDS             <1..1704
                     /gene="adeD"
                     /locus_tag="ACHOQW_004043"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9575022.1"
                     /translation="AVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVGAE
                     YTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHEIV
                     NVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQVT
                     GLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIENCHESYQ
                     LEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEGHI
                     DALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVTVQ
                     QVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVIDV
                     IHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRLAPACGLLGGFGLNEGALAATV
                     SHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTAQS
                     LAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLEVT
                     E"
     gene            complement(1750..3141)
                     /gene="uhpT"
                     /locus_tag="ACHOQW_004044"
     CDS             complement(1750..3141)
                     /gene="uhpT"
                     /locus_tag="ACHOQW_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP9575023.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3279..4598)
                     /gene="uhpC"
                     /locus_tag="ACHOQW_004045"
     CDS             complement(3279..4598)
                     /gene="uhpC"
                     /locus_tag="ACHOQW_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9575024.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4608..6110)
                     /gene="uhpB"
                     /locus_tag="ACHOQW_004046"
     CDS             complement(4608..6110)
                     /gene="uhpB"
                     /locus_tag="ACHOQW_004046"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP9575025.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6110..6700)
                     /gene="uhpA"
                     /locus_tag="ACHOQW_004047"
     CDS             complement(6110..6700)
                     /gene="uhpA"
                     /locus_tag="ACHOQW_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP9575026.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6862..>7407)
                     /locus_tag="ACHOQW_004048"
     CDS             complement(6862..>7407)
                     /locus_tag="ACHOQW_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575027.1"
                     /translation="EIFKKVVFTPGECDLVRDSLVQDGIKPEKIAKIISCLSSRTGIR
                     NLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNYHQHVDFSEYGFT
                     QTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSDESYRHKDDYWAR
                     DNDIRNLRRNFSGNITIATYK"
BASE COUNT         1888 a         2091 c         1893 g         1535 t
ORIGIN      
        1 gccgtttccc gtggcgacgc tgttgccgat tatattattg ataatgtctc tattctcgac
       61 ctgattaatg gcggagaaat ttccggccca attgtgatta aagggcgtta cattgccggt
      121 gttggcgcag aatacactga tgctccggct ttgcagcgga ttgatgccca cggcgcaacg
      181 gcggtgccag ggtttattga tgctcacctg catattgaat ccagcatgat gacgccggtc
      241 acctttgaaa ccgccaccct gcctcgcggc ctgacaactg ttatttgcga tcctcatgaa
      301 atcgtcaacg tgatgggcga agccggtttc gcctggtttg cccgctgtgc cgaacaggca
      361 aggcaaaacc agtacttaca ggtcagctct tgcgtacccg ctctggaagg ctgcgatgtt
      421 aacggtgcca gttttaccct tgaacagatg ctcgcctggc gggaccatcc gcaggttacc
      481 ggccttgcag aaatgatgga ctaccctggc gtaattagcg ggcagaatgc gctgctcgat
      541 aaactggatg catttcgcca cctgacgctg gacggtcact gcccgggttt gggtggtaaa
      601 gaacttaacg cctatattgc tgcgggtatt gaaaactgcc acgaaagtta tcagctggaa
      661 gaaggacgcc ggaaattaca actcggcatg tcgttgatga tccgcgaagg gtccgctgcc
      721 cgcaatctca acgcactggc accgttgatc aacgaattta acagcccgca atgcatgctc
      781 tgtactgatg accgtaaccc gtgggagatc gcccatgaag gacacatcga tgccttaatt
      841 cgccgcctga tcgaacaaca caatgtgccg ctgcatgtgg catatcgcgt cgccagctgg
      901 tcgacggcgc gccattttgg tctgaatcac ctcggcttac tggcacctgg taagcaggct
      961 gatatcgtcc tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact ggtgaaaggc
     1021 gaaccgattg acgcgcaaac cttacaggcg gaagagtcgg cgaaactggc acaatccgct
     1081 ccgccatatg gcaacaccat tgcccgccag ccagtttccg ccagcgactt tgccctgcaa
     1141 tttacgcccg gaaaacgcta tcgggtcatt gacgtcatcc ataacgaatt gattacgcac
     1201 tcccactcca gcgtctacag cgaaaatggt tttgagcgca atgatgtgtg ctttattgcc
     1261 gtacttgagc gttacgggca acgtctggct ccggcttgtg gtttgctcgg cggctttggt
     1321 ctgaatgaag gcgcgctggc tgcgacggtc agccatgaca gccataatat tgtggtgatc
     1381 ggtcgcagtg ccgaagagat ggcgctagcg gtcaatcagg tgattcagga tggcggcggg
     1441 ttgtgcgtgg tacgtaacgg ccaggtccaa agccatctgc cgttacccat tgccgggctg
     1501 atgagcaccg acacggcgca gtcgctggcg gagcaaattg acgccttgaa agccgccgcc
     1561 cgtgaatgcg gtccgttacc cgatgagccg tttattcaga tggcgtttct ttccctgcca
     1621 gtgatccccg cgctgaaact aaccagccag gggctgtttg atggcgagaa gtttgccttc
     1681 accacgctgg aagtcactga ataataaaaa aagcccggcg tcatgccggg caaaagtcac
     1741 cagttacgtt tatgccactg tcaactgctg aatttttttc tcgcggcgga ttttgcgttc
     1801 ttccatcacc gccactatcg ccatcaggca gatacaacca atcgcggcga tatccagcgc
     1861 agcgaaggtg cctgcccagc cggtaaggcc gaatactggc gtcccatcgg caatcattcc
     1921 cagacctaac ttggcaaagc tgtcaccaat caggtaagcg aaggtgcctt taataccatc
     1981 ggcagcgcca atcgcttttt taggtacaaa gccaacagca gccacaccaa tcaacaattg
     2041 cgggccaaag accaggaaac ccaacgcaaa gagagaagcc agatagatat attcgttgct
     2101 ggcatgttgg tacacaccga gcgtggcgat aatcagcgcc agcgcgatgc aggccaccag
     2161 gccacggcga ccgttcgcta ggtcagagag ccagccccac agcagcgtac cgaccagcgc
     2221 accagcttca aacagcgtaa agccctgaat cgccaccgct ttagagagtt tcagttcctg
     2281 gaacgcgtac acagttgacc actggtcgat accaatacgc accacataga ggaaaatgtt
     2341 ggcgaagcac agcagccaga tcactttgtt tttcagcaca tactcaacaa agatctgcca
     2401 cttggtcata tcggtagatt ctgtctcttt gtcctcttcg ctgatctcct cgccgaacag
     2461 ctcttcagct ttgccgaggc cataagattc cggggagtcg ctgccgtaac gcaggccgat
     2521 aaaaccgaca atcagcgcga taatcgacgg gaagataaac atgccgatga catggccatc
     2581 gaacaggtaa tttgcgccga acagtgccac accagctgcg cctgcaccgc caaggttgtg
     2641 agaaatgttc cagaaaccga ggaacgtccc gcgtttacga cgcggcgtcc atttggtgat
     2701 ggtggagtaa ctgcacgaac ccccggtact ctggaaaaag ccacttaagg cgtagaaggc
     2761 aatcatcagg aacaggctaa ccgagccgct gcccatactg gcgctaaagc ccagcataca
     2821 aatggcagac aggatcagca tgaacggcag gaattgtttg gtgtttttgc cgtcggcgta
     2881 gtaggaaacc agcgttttac ccacgccata agtgatggag aaacccaggc cgatcatccc
     2941 cagctgcgtc atgctcaacc cgtaggtcga aatcatatcg ttctgcgcga tgttaaagtt
     3001 cttgcgaatc aggtacatcg tcaggtagcc gataaagacc accaggtagg attgcatgaa
     3061 cggtttgaac cacattttgc gccgcacttc gagcggaagg tccagggtcg gcttgcggac
     3121 ctggtttaag aaagccagca tgggttactc ctgaaatgaa tacctgcgtg aggcatgcat
     3181 tgtaaaaatc agcagcaaat caggcctgag atagcgtcca ggcaaaacct ggaaaatttc
     3241 ttagttttgc cggatatgaa gtgaaaaggt gagatgcatc acgcttcgcg cggtgtctgg
     3301 gcgttcaaaa agggcagtaa cagcagtgcg gaaatcccgg cggcaatggc gataaccaca
     3361 aaaaatccgc tccagtgcca ggtatcgagc actttcgcca gcggccaacc agcaagcgac
     3421 gcccccagat aagcaaacaa gccgacaaac cccgtcgccg cccctgccgc ctctttgtgg
     3481 gaacattctg ccgccgccat gccgattaac atctgcgggc caaagacaaa aaaaccaatg
     3541 gtgaagaagc aggttgcctg catcacgtag ctggcaaatg gcatcagcca cagcgagccg
     3601 actgaaagca aaattccggc agcgaaaatc aaattcatcg gcccgcggtt gccgttaaac
     3661 agtttgtccg agccccaacc cgctaccagc gcaccgataa atccgcccag ttcaaacatc
     3721 gtcactgccg tattcgccgt gaccagatcg acgcccagcg tctcggacat atacaaattg
     3781 ccccagtcgt tgatcgccgc ccgaaccaca tagaccagca catagcaaaa cgaaagcagc
     3841 cagatatacg gattcaacaa cacatacttg gtgaggatct ctttacgcgt cagccctgcc
     3901 ccttcttgtt gttgagcaat ctccagcgcg tcgtgtcgcc attcaccgac cgctggtaaa
     3961 cctaacgcct gcgggcgatc gcgcagccgc cagcagagaa aaatccccac gacgatcgcc
     4021 atacaaccag caatcatcat cccggcacgc cagccgtaat gcagcgcagc tgctgccatc
     4081 acaatgggaa tgagcgcacc gccaacgtta tgcgccgtgt tccataatgc ccaccaacca
     4141 ccgcgttcgg tacgagaata ccaggccgtt aacagacgcg cacacaccgg tgaaccccag
     4201 ccctggaaaa aggcgttcag cacccagagc acggcaaacg cccatagcga cgtcgagaag
     4261 ccaaacagaa tgttgatgat gcccgtggcg ataagcccta tccccataaa ataacgggca
     4321 tttgagcggt cgctgacaat gccggagaca aacttcgaca cgccgtaggt aatgtaaaac
     4381 agggtcgcta acaggccgat atcgctacgg ctgagcacgc cattagcaag gatttctggt
     4441 acggcggcgt taaaactttt acgcgtgaag taaaacagcg cgtaacccag ccagatggtc
     4501 agcagaatat gccgacgcca gtagcgataa cgggcatcaa tttcatgttt atcagtcatt
     4561 aatggcgcat cggcaggcgc tttcagaaac ggcaacatca tcaaacctta gacatagcgt
     4621 tgaggtagag aaacgctgac acgcgtgccg tgcagacagg aaatggtcaa ggtgccgccc
     4681 agcgccgtta cgcgctcgcg cattccggtg aggccaaaac cttgttgccc ggaacccggc
     4741 ggtaaaccgc tgccatcgtc ttcaatcacc agcatcaacc gttcatcctg cagccagcct
     4801 tgcagggtga ccgcgctggc atcagcatgt ttcacaatgt tattcagccc ttcctggcag
     4861 acacgaaaca gcgtcacgcg ctggttttcg cttaacgctg actcttcgat tcgccattcg
     4921 agatggctga caatgccgcg cccttccagc tccatttccc gcatcagtga gcggatggcc
     4981 tgctctaggg tgagatcatc caactggcgc ggacgtaacc gccctaacaa acggcgcacc
     5041 gcgtcgtaca cgcccagcga cagttgttcg atgagctgcc cgctctgctt cacactggcg
     5101 ttatctgccg ccagtcgctg gacaatgccc gcttgagtac gaatggcagt gatggtctgg
     5161 ccgatatcat catgcagctc acgcgccaca tcacggcgca cgctctcttc agtttctaac
     5221 aaacgttcag cgagatgctg attgcgcgcc agttcctttt gcagcgactg gttaagttca
     5281 cgcaaccgct ggatgccagc gcccagtaac aaccctgtca gactttgcac cagcagcgag
     5341 agcaataaat ccacaggatg atcgcgccag gtttgactgg cgatcagcgc gatggcgttc
     5401 atcaatgtcg ctatcagcgc cccttgccag ccgtagtgcc aggccagcgc gataatcggc
     5461 agcgccaggc agaatggcgt aaagcgcgac agttcgtccg gcaagcccaa ctggagccag
     5521 agactgataa caaacagcag caagtaccag accagatgcc gcccgcgcca gttgatcggt
     5581 tgagaaacta acgacggccc gagcggcagc caggtgttat tggcgagata atgccagaac
     5641 accagacata tcggggccag cgtcaggccg ccagttaaag ttaacagcag cgcattccac
     5701 gactctttgc cgtgccaaag ccagggcagc gactgcaaca acgccgccgc cgttaacgcc
     5761 gcaccctgta acagcaaggt gcgccagtca cgttgatggc gatagcgcga gatcagcgcc
     5821 acgggcagta acgtcagtaa actaccgatc atcaataagg agaaatgggt taaaccgacc
     5881 gcctgcgtta gccagtaaat cagcagccac tccgctccca gcagcaccgg ccagtatccg
     5941 cgcgggcatt gcagcattag ccctagccgc agaccaaacg gaaataacag caccgccata
     6001 tcagggcgct caaccaggtg caggctgata ctccacaggc aaaaccatgc ggcagagaag
     6061 ataaaaaagc aggcaataac ggtaattaag cgggagaaca acgtcttcat caccagccat
     6121 caaacatacg gcgcgccagt tcaacgtcgt tactgacgcc cagtttttcc atcagattgg
     6181 cgcgatggac gtgtaccgtt ttcggtgaca agcccagttc ggcggcaatc tctttcaccg
     6241 ccattccttg cgccagtttt tccgccacct gccgttcacg tttggttagt gggtcctggc
     6301 gaccggatgc cagtttaatg gcaatatccg gcgtcagata acagccgcct gtggcaaccg
     6361 tatgcaccgc agcaatcagt tcgtcagggc tacaacgctt ggagagaaag ccacgcgccc
     6421 ccgcgttaag cgcctgctca accagcgccg gactgtcatg aacggagagc ataattgtcg
     6481 ccataccttt cggcagctgg cttagcagct ccagaccgga gatatcgggc atggagatat
     6541 cgcaaataca cacctgcaca ccgcgccccg gcagccccgc cagcgcctcg cgccccgaac
     6601 caaactcggc aactacttgc aaatcaggtt ccagccccag caactgcgca aagccggagc
     6661 ggacgatgag gtgatcgtct ataagggcaa cggtgatcat gattgggtcc tggcggataa
     6721 aaaacgcgca taccttaacg ataagcgcga tgttgttcaa gttggatatg gggtatgtat
     6781 tggcttaatg atgttgagct tatcgtcaaa acgcttacct gagaggtaag cgtttggtgc
     6841 gtgttattga gaaaaagcga attatttgta ggttgcaatc gtaatattgc ctgagaaatt
     6901 tctccttaga ttgcggatat cattatccct tgcccaataa tcatctttgt gtctataact
     6961 ctcatcggaa tgtacatagg gagaatcatc ttcagcgtcg ttatggataa tattttcgcc
     7021 tttagtggta tagatataat tagcaacatc aaaggtgtca ttcgcgttat ttacgactgc
     7081 gcctaagacg caaagggttt gagtgaatcc gtattctgag aaatcgacat gctgatggta
     7141 atttctacca ttcaaagcaa caaaactcgg acaaacatct attgtatcgc caacagacgt
     7201 agcggcgcga cgcacatcaa gtgcagcgtt atcgttagca acatgaaatg cttgtgaaca
     7261 ggcataaaac aaatttctaa tcccggtacg actggataaa caagaaataa tttttgctat
     7321 tttttctggc tttataccat cctgtactaa agaatctctc accagatcac attcaccagg
     7381 tgtgaaaaca actttcttga atatttc
//
LOCUS       DAZKFZ010000216         7379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000216 DAZKFZ010000000
VERSION     DAZKFZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.216"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(142..426)
                     /gene="ppnP"
                     /locus_tag="ACHOQW_004049"
     CDS             complement(142..426)
                     /gene="ppnP"
                     /locus_tag="ACHOQW_004049"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP9575028.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(498..1175)
                     /locus_tag="ACHOQW_004050"
     CDS             complement(498..1175)
                     /locus_tag="ACHOQW_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP9575029.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1433..1624)
                     /gene="yaiA"
                     /locus_tag="ACHOQW_004051"
     CDS             complement(1433..1624)
                     /gene="yaiA"
                     /locus_tag="ACHOQW_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP9575030.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1674..2198)
                     /gene="aroL"
                     /locus_tag="ACHOQW_004052"
     CDS             complement(1674..2198)
                     /gene="aroL"
                     /locus_tag="ACHOQW_004052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP9575031.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2381..2839)
                     /locus_tag="ACHOQW_004053"
     CDS             complement(2381..2839)
                     /locus_tag="ACHOQW_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9575032.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2959..3768
                     /gene="proC"
                     /locus_tag="ACHOQW_004054"
     CDS             2959..3768
                     /gene="proC"
                     /locus_tag="ACHOQW_004054"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9575033.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(3785..4900)
                     /gene="adrA"
                     /locus_tag="ACHOQW_004055"
                     /gene_synonym="dgcC"
     CDS             complement(3785..4900)
                     /gene="adrA"
                     /locus_tag="ACHOQW_004055"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP9575034.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGIWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(5002..5322)
                     /gene="psiF"
                     /locus_tag="ACHOQW_004056"
     CDS             complement(5002..5322)
                     /gene="psiF"
                     /locus_tag="ACHOQW_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP9575035.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5441..6856)
                     /gene="phoA"
                     /locus_tag="ACHOQW_004057"
     CDS             complement(5441..6856)
                     /gene="phoA"
                     /locus_tag="ACHOQW_004057"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP9575036.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(6957..7217)
                     /gene="iraP"
                     /locus_tag="ACHOQW_004058"
     CDS             complement(6957..7217)
                     /gene="iraP"
                     /locus_tag="ACHOQW_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP9575037.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1800 a         1926 c         1755 g         1898 t
ORIGIN      
        1 tcttcaatga tatttatatc aaccttgatg aattatgaat atatcggaaa ttgaaaaaat
       61 cgcagcaaag gaaatattat tatggggtta tgagaggaca tttcagaaaa atgccctccc
      121 gttcaagcgg aaggcgagga attacagata gcggcacaga taagaggtgg gttcggcaac
      181 ttgcagatga aactcactgt gaccgggaac attaaacacc gaaccggctt catacacttg
      241 ccagtcggtc gcgtcaggca gtaacacatt cagcgcgcca ctgattaccg tcatctcttc
      301 cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacgc tggcgcgacc
      361 agtgctgctg ctggaaaagc cgatcgattt cactttgccg gaaaagtact cattactttg
      421 aagcataaac tggccctgtc tggtagttga atatctactt cactataggg gccagatgcg
      481 ctggctgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat caatacgtta
      541 gagagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg ctggttaaat
      601 cccaaacaat ccagcatgat gacatctgcg cctttcgcca gtaattcttt cccggcatca
      661 atgatttttt gttctgaatc atgaatgggg ttacccaatg aaaatacagg cggtttctgc
      721 aaaatttgcc atttttgcgc ctgaacggtc agcaactcct caaccggtac gataaccccc
      781 acctgatgat cttcaacaat agaggaaacc agcggtggca atatccgcga cggctcaaga
      841 aagatcgtat tacgcgcagt catactacta atgtttgccg tactcattaa tataatgacg
      901 tcgtagccct gattatcgag cacttcaacc acaccttgca ggtcacgctc tactttgcga
      961 cgcgaaacat gggccagatg gttgtcattt aataatgtga gaattgtgtc ttcgcctgct
     1021 tctggcgcgt actccgccat cacttcttcg cggcttaact tccccagcag gctatgatgg
     1081 gaaatattat cttcgtcaat gtattctgtc aggagcggca aaacttcctg cataggtaca
     1141 atgccgatgg ttagaatcgc caacgacgca ctcattgttc ttccacctta cttttatttc
     1201 gtcgttaatt accgggcaag tgttaagcac cactgctgac acttacttgt gctacaaatg
     1261 acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat gattccagta
     1321 atggattgtt attgtttaat attctaatta tttgaaaaac atgaattatg aaaaatgtga
     1381 cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc ggtcactctt
     1441 tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagcggtc ggatgttcac
     1501 tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt accgtctgtc
     1561 ctggctttcc tttttcaatt gtcactatat atgcttcacg aggataaggc ggtttcgttg
     1621 gcatcgttct tccttatttc acgggatgaa cgttaagtat aggcgctcga aaatcaacaa
     1681 ttgatcgtct gcgccagggc gctgcgaatt tcagaaatca cctggctggg ttcgtttgtt
     1741 gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc cagcacttcc
     1801 tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc ttccggtgca
     1861 gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac gatcccgtta
     1921 ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt agcgataacg
     1981 gtggatggcg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa tcccgcccac
     2041 tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa ccactgatcg
     2101 gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt tgttttacca
     2161 cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc ccaataggtc
     2221 gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat accatcaaac tagtacaatt
     2281 tcgattgtaa agaaaaaatt ccacttaaag tgaaaatctc aatacatccc ttactatacc
     2341 aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga ctacgttgca
     2401 cttccagcca ccacttctcc agctccgcgg caaaggcctg gcggtcacgt tgtgaaaggc
     2461 tatctggtcc gccggtctgg atcccactgg cacgtaaggt atccataaaa tcgcgcatcg
     2521 tcaggcgctc acgaatggtc gctggcgtgt aacgttcacc gcgcggatta agcgccgcag
     2581 cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc aaatctcccg
     2641 cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg acgcgcagcg
     2701 tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt accagcggca
     2761 tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga cacgcgtcgg
     2821 catccaccca aattgtcata aagtcatcct ttgttgggta atcctctatt gtgtcgcgct
     2881 tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa caatgaattt
     2941 cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg caatatggga
     3001 aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca aatctgggta
     3061 tataccccct ccccggataa agtcgccgcc ctgcatgaca agttcggcat caacgccgca
     3121 gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgccgccgt taaacctggc
     3181 atcatgatta aagtgcttag cgaaatcacc tccagcctca ataaagactc tctggtcgtt
     3241 tctattgctg caggtgtcac gctcgaccag cttgcccgcg cgctgggcca tgaccggaaa
     3301 attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac ctccgtaacg
     3361 ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt ccgctgcttt
     3421 ggcgaagcgg aagtaattgc tgagccgatg atccacccgg tggtcggtgt gagcggttct
     3481 tcgccagcct acgtctttat gtttatcgaa gcgatggccg acgccgccgt gctgggcggg
     3541 atgccacgcg cccaggcgta taaatttgcc gctcaggcgg taatgggttc cgcaaaaatg
     3601 gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc accgggaggc
     3661 accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc agtgatcgaa
     3721 gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg actttcgccg
     3781 gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg tagagcgcca
     3841 aatctgccga tttcaaccac tcgcgatagt gactcatttg tgggttcagc ggcgcaaccc
     3901 ccacactaat ccgtaaattt acctgtggcg tattcggcaa acgtaatgta tttagccctt
     3961 catgcacccg taacatggcg gtaatggcgc tctcagctgg cgtaccggac atgattaccg
     4021 caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta atttgtaact
     4081 gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg gccccaggta tcgttgatgc
     4141 tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgc cgccgacagt
     4201 tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg cccgtcatgc
     4261 cgtcgcgggt actcatgacc tgcaacctgc gtttatgttc cgccagtttg gttgccgtct
     4321 ggtagctgac ccagccaaac agcagaggat aaatgacaat aatgggaagg gagagccacc
     4381 attccagcgg cgcactattg aacgacacgg taatgcccgt cagctcgagg gtgacaaggc
     4441 aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct gcccccatca
     4501 aattcagaca cataatcatc aacatcgcgg tggaaggcag cacgtttacg cccattacgc
     4561 ctacccatat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc cggctaagcg
     4621 gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac gcccagccga
     4681 ccaacaccag ccaccaccag cccggcggcg ggtgtgaaac cagcgttgaa gcaatcggta
     4741 agaacatgcc agccaggcca accgcacggg gtagtctgac gcgacgggcg aagcgcagcc
     4801 cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccgtgc gccgccgctt
     4861 ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa ttttcccaaa
     4921 ttatagagac ggggctaaag gcagaagtat gaaatttcgg gtgagccatt ttagtagctc
     4981 acccgttagc actatcaggt atcaggcggc tttcttgagg caggcgctca taaacttatt
     5041 acgatcatca cctttcagtg attgttgcgt tgcttgatta ttacattcgc gcatcttttg
     5101 ctgctgtggc gtcaaacttt tttcaccagg cgcagacttg ctgttcttca ggcaatcact
     5161 catgtaggtc ttacgagcat cccctttcaa cgcctgtgcc gtcgcctgct gattacagga
     5221 ggtcatacgc tgttgttgtg gggttaaggt tctctcggca gcgccgacgg tggttaaaaa
     5281 aaccagaccg aaaagcaagg taaccagtaa tgtaattttc atagcaccat ccctcttcat
     5341 gttttaacca tgagcgtatg cgcccgtgat ctgacattaa gtctggttgc taacagcaaa
     5401 aaaaccaccc ggcagcgaaa attcactgcc gggagcggtt ttatttcagc cccagagcgg
     5461 ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacgacattg gcggcatgcg
     5521 ggccatacgc cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc tcttcggagt
     5581 tcccgtaact catcaccatc actgcgccat ctttggtatt tagcgcctga gtgaggcccg
     5641 gagctttggt atccggcgca acaatctggc tggcgtgggc gtgatcagcg gtgactatga
     5701 ccagcgtgtt accatccttt ttggcgaatt ccagcgcccg ttgtacggct tcatcgagat
     5761 cgaccgtctc gccaatttgc ccacaaggat tcgcagcatg atcctgttta tcgattgacg
     5821 caccttcaac ttgcaggaaa aagcctttct catttttact caacaattca atggctttgt
     5881 cggtcatctg tgccagggtc ggtacactgt cattacgttg cggattaggc gtacaggtga
     5941 ctgcgggctt atcgatatta ccgtggtacg ttgctttcgg tcctagccag cgcactggca
     6001 tattgccgtc agcaaacagg ccaagcaggg gtttttgctg attcgcttcc gtcaccgaat
     6061 tcagtgaggc agcatcgctc accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg
     6121 tttttccctg ccattcacca gcggttgccg tttcagcaaa ggtttttgcg ccgccgccaa
     6181 gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt
     6241 ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgagg
     6301 tcacatgcgc caccagcgcg gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac
     6361 cggtcgccag acctgcggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa
     6421 tatcgacgcc cagcgcgccg ttataggttt tgacaccggt tgaccaggcg gttgctgatg
     6481 cagccgagtc ggtgacgtag tccggttttc cggttttttt attcagcgca tagtgagtgt
     6541 attgcccggt aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat
     6601 aattacgtgc ggcagtaatt tccgaatccc ccatcccatc gccaatcagc aaaataatat
     6661 tttttgcagg tttatcgcta agagaatcac gcagagcggc ggtctgatca gccgttaagc
     6721 ggcgagcacc gccgggtgca gtaatatcgc cctgagcagc ccggttttcc agaacaggca
     6781 tttctggtgt ccgggctttt gtcacagggg taaacagtaa cggtaagagt gccagtgcaa
     6841 tagtgctttg tttcacttta ttttctccat gtacaaatac attaaaaaat aaaaacaaag
     6901 cgactataag tctcggccgt gacaacttta tgacagctgt tgaaaagatt aactttttac
     6961 tgacgaggat gcttcaataa cttctttacg taatcgcgca gcagctccgt atcgtcgtca
     7021 ggaatgctgg catcgggctt tacctcgtac agcgccccct ctacctgatc aatcaaccgc
     7081 tgttggtcat tttgcgccat attgcgaagc attgcagtga cgataatctc caaagcttct
     7141 acctgcgcac acagttcttt cgactcttct tctttttggg caagcttaaa taacaactca
     7201 gcaatgagat ttttcatgtc tgtatttcct tatccaaagt atggagaagt taacattatg
     7261 tttcacaact tcatagtgtc tggaggtatt atttttatgg caaaggaaat actttgcgca
     7321 aatatttttt gtttgattac cagcgaaacg tttctcttat ttaaataatt acccacaca
//
LOCUS       DAZKFZ010000217         7357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000217 DAZKFZ010000000
VERSION     DAZKFZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..873
                     /locus_tag="ACHOQW_004059"
     CDS             <1..873
                     /locus_tag="ACHOQW_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9575038.1"
                     /translation="VDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMISKVERGTTDA
                     GAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRGGHGSVDSYSLGGYA
                     SWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLGGHIETGMRFTDGNWN
                     LTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLK
                     AAVRKEFVDDNRVKVNSDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLGVGYSNGAGM
                     ESPWNAVAGVNWSF"
     gene            complement(1245..1511)
                     /gene="minE"
                     /locus_tag="ACHOQW_004060"
     CDS             complement(1245..1511)
                     /gene="minE"
                     /locus_tag="ACHOQW_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP9575039.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1515..2327)
                     /gene="minD"
                     /locus_tag="ACHOQW_004061"
     CDS             complement(1515..2327)
                     /gene="minD"
                     /locus_tag="ACHOQW_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP9575040.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2351..3046)
                     /gene="minC"
                     /locus_tag="ACHOQW_004062"
     CDS             complement(2351..3046)
                     /gene="minC"
                     /locus_tag="ACHOQW_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP9575041.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3566..3934
                     /locus_tag="ACHOQW_004063"
     CDS             3566..3934
                     /locus_tag="ACHOQW_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP9575042.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4037..4438)
                     /gene="pliG"
                     /locus_tag="ACHOQW_004064"
     CDS             complement(4037..4438)
                     /gene="pliG"
                     /locus_tag="ACHOQW_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP9575043.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4509..4667
                     /locus_tag="ACHOQW_004065"
     CDS             4509..4667
                     /locus_tag="ACHOQW_004065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575044.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4679..4972
                     /locus_tag="ACHOQW_004066"
     CDS             4679..4972
                     /locus_tag="ACHOQW_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP9575045.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5044..5703
                     /locus_tag="ACHOQW_004067"
     CDS             5044..5703
                     /locus_tag="ACHOQW_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9575046.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5795..6241
                     /locus_tag="ACHOQW_004068"
     CDS             5795..6241
                     /locus_tag="ACHOQW_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP9575047.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6448..7357)
                     /gene="hlyE"
                     /locus_tag="ACHOQW_004069"
                     /pseudo
     CDS             complement(6448..7357)
                     /gene="hlyE"
                     /locus_tag="ACHOQW_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         1888 a         1626 c         1779 g         2064 t
ORIGIN      
        1 gtcgatagcg atattgcacg tttgttactc aagctcagaa agtctcgaaa cttgactgtt
       61 acagagcttg cccaacgatc tggtgtcagt caggcgatga tcagcaaagt tgaacgaggc
      121 accactgatg cgggtgccgg gtttgagcag acgctgaccg gaatgacagt ggggatcgac
      181 agccgtaatg atattcctga ggggattgcg acgctgggcg cttttatggg ttattcccat
      241 tcacatatcg gttttgatcg tggaggacat ggcagtgtgg acagttattc tctgggcggc
      301 tatgccagtt gggaacatga aagtggtttc tatctggacg gtgtcgtgaa gctgaaccgt
      361 tttgaaagta acgtagccgg taaaatgagc agcggtggag ccgccaatgg cagttaccat
      421 agcaacgggc tgggcggtca cattgaaacc gggatgcgat ttaccgatgg taactggaac
      481 ctgacgccgt atgcctcgtt aacggggttc accgctgata accccgaata tcatttatcc
      541 aatggcatgg aatcgaaatc agtcgatacc cgcagtatat atcgtgaact gggtgcaacg
      601 ctgagttaca acatgcgtct ggggaacggt atggaagttg agccgtggct gaaggcggct
      661 gtgcgcaaag aatttgtcga tgataaccgg gtgaaagtga atagtgacgg taatttcgtc
      721 aatgatttgt cgggcagacg tggaatatac caggcaggta ttaaagcctc attcagcagt
      781 acgttaagcg ggcatcttgg ggtggggtat agcaacggtg ctggtatgga atccccgtgg
      841 aacgcggtgg ctggtgtgaa ctggtcgttc tgaccatcaa cgattaaact gcgcttcggc
      901 gcagttttcg tttacaggat gttaaaagag aaaattctgg ggtaaaaaaa gcccgccagt
      961 tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg gggttatcat
     1021 ctcccagtat atcaacacta acaataaggt tatttactca accaggcata aatattttgt
     1081 tttgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca atgaggagta
     1141 tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa aactcaaggc
     1201 agagataact ctgccttgaa gataaatgcg cttttacagc gggattattt cagctcttct
     1261 gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt ttgctcaagc
     1321 tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc aagaatatct
     1381 ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc agcaacaata
     1441 atctgcagcc gttcttttgc aatgttggct gtatttttct tccgcgagag aaagaaatcg
     1501 agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt ctcttcttca
     1561 atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc gtaggcttta
     1621 cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc gcgcaatact
     1681 gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag cacatcttcc
     1741 atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc
     1801 tctttaatag gctcttcgcc attttctgcg cggcgtgatt ttgacgccag aatgcctaaa
     1861 atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat ggcttcgtct
     1921 gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc acaaacgata
     1981 aattcaaaat ccatcgcttt cagatcatca agaactttag cgaccccttc acgggtgagg
     2041 gcatctttat cgcgtgtttg cgatgccggc agaatataga gattttcagt acgcttatct
     2101 ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg
     2161 acccggcgtt cacaacccat aatcaggtcg agattacgca ggccgatatc aaaatctatc
     2221 acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc
     2281 ttaccaacac cccctttgcc cgaagtaaca acaataatgc gtgccataga aattccttgt
     2341 taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg
     2401 ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca cctgcgatgg
     2461 acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc
     2521 ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg gcaatcaatt
     2581 cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca taaatacgct
     2641 gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtt gtattttgcg
     2701 ctggagcctg cggtgcggga gctggacgtg gcgctttttc ctttccttcc gtcaggatag
     2761 gcagccccat tttttcaatt tcggctttaa gttgcgcatc tttgcagcca cttacgccaa
     2821 taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt accgggtctt
     2881 ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg gcctgagcga
     2941 ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga tgaaccacag
     3001 ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg gccttactca
     3061 attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag gcatgttata
     3121 gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag tctattctgt
     3181 gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc attgccgtcg
     3241 tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg acagcatctc
     3301 cagacctgtt taaatatcca gtattgaggc aagtgtattt tttcaccacg ccaggctttc
     3361 ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct taatatccgc
     3421 cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt caggtcatcg
     3481 taactcgtcc aggtcagcgg ttttgattac ggattgatga tgaaatgagc ataagtgttg
     3541 tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attttttata tcggattgtc
     3601 aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg gacttcaatc
     3661 tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg gcatttcccc
     3721 agagttaaca aggaaatatc tcagcgaaaa agccgctgaa aacttacaat cattacaagg
     3781 ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt
     3841 atgccgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa tcaatcaaac
     3901 caccacaaag atgttattta tgtgtcgtga gtgatgtctt gtttacatta tgttctattc
     3961 gtcattaacc tcttgagcga cagagaggag tggggcgtca acgtttatga cggggagact
     4021 ccccgtcggt tgacatttac ttaatttgaa tatcgacgtt atatttttta gttttgtttt
     4081 tacgggcgtc gtttcgggtt tgcagcactc gcagctcata tttgccagaa gcagggagtg
     4141 agtattgtcc gtgactatcg agttcagggg aatatctgga taaatcaaca gaatcatcga
     4201 ttcctggccc gaagaggtaa gtatcagcgc cttcattaga gatactcaca tgcactttct
     4261 gacctttttt ggcatagaag gtatatgtat cgtaatcgta tccctttatt tcgccggagt
     4321 actgagcgct gctatggcct tttctgaact caacattgac attcttaccc gcagcaaagc
     4381 tcgtggaggt tagcagtgca agcagcaata cagccttact gatgctcttg attttcattg
     4441 gtagtcttct gttcagaatg agtacgatga actgttataa tataacaatc ccttacggga
     4501 gaattccagt ggctttcctg cgttcggtaa aatcccgtcg cggcattttt gccgggggga
     4561 ttacttataa aggggcatgg actcaacaaa ccgtagaaat ttacagctgg ctggcttgtg
     4621 gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga gtaaaagtat
     4681 gttttgtgtg atttatcgaa gcagcaagcg tgaccagacc tatttatatg tcgaaaaaaa
     4741 agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc ctcagttagc
     4801 gatgattctg ccgctggatg ggcggaagaa actggtcaat gccgatattg aaaaggttaa
     4861 acaggcatta accgagcaag gttactattt gcagttaccg ccaccacccg aagatttgct
     4921 gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat aaccgatatc
     4981 cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg tgaggggaac
     5041 gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt gagtaaagta
     5101 gtttgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc agtgcccgaa
     5161 gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca gccattggcg
     5221 atcccatccg atttcggttc agttcatcat gaagtcgaac tagcggtgtt gattggcgcg
     5281 acgctgcgtc aggctacgga agagcatgtc cgcaaagcca ttgccggtta cggcgtggcg
     5341 ctcgatctga cgttgcgtga tgttcaggga aaaatgaaga aagccgggca gccgtgggaa
     5401 aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttattcccgc ggcggaattc
     5461 accggcgatc cgcaaaatac aacgctgggc ctgagcgtaa acggcgaaca acgccagcaa
     5521 ggtacgactg cggacatgat ccataaaatc gttccgctga tcgcttatat gagcaagttt
     5581 tttaccctca aggccggtga cgttgtgctg acaggcacgc ctgatggcgt cggcccgttg
     5641 caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac tcgcgttttg
     5701 taatactttt tgccgcctga aagcggcggc aaaacttgca tcgctgtgcc agactggtta
     5761 taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt tctggcaaag
     5821 taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg gttgcggtca
     5881 gtgttgcctg cataaactga tggatgaaga caccgacgaa atctacttca ctaacgtcgc
     5941 ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc gtttcgagtt
     6001 tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat ggctaccaat
     6061 gacctgcgct tatcgtttgc tggcggaagg taaagattta cctgcgtggc atccgctgct
     6121 tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata tcgcagtgaa
     6181 agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact gggcgcagtg
     6241 ataaatttaa tagatgttga tgggggcttt cttaatatac gtctggatgc tcgccatttc
     6301 gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc ttattatcaa
     6361 caggtaactc tcaatcttta aaaaacgtct gtatttttaa atgtgataaa ctttatacct
     6421 tgagtacatg gagagaataa ttgctgatca gacttcaggt acctcaaaga gtgtcttctt
     6481 accgtgtctt ttctgatact cattacaggt gttaatcatt ttattggccg cttcttttag
     6541 caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag tttccgtttt
     6601 tatctccccg atggcgctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta
     6661 ttcgcttgtt taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg
     6721 ttcttcaatt ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg
     6781 attaaaccaa atggaccggc gacgacaccg gctgcggcac cggcatacgc ttccttcctg
     6841 attttatcta cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg
     6901 ctatctaacg ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg
     6961 gatttttgcg cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gagaatgtct
     7021 ttctgggcgg atgctttctt ctcattatac tcatcaaata gcaaaatata cgctgcgagc
     7081 aattgcgtcg caacaccaca ccattcatac actgtttggg ttgcttcaaa atacttatcc
     7141 tggctatcca ttagtaaggt tttaatattg ccaactaaaa cggaggctgc ctgtgaatac
     7201 tcctgtttaa agcgacttaa ctcttttatg gtttcatcaa aggtctgcca ggggatgacc
     7261 tgatcgagat atttattata aagatccaat gctccatctg cggtttcgat tgcgttttta
     7321 actacctcta ccgttttatc tgcaacgatt tcagtca
//
LOCUS       DAZKFZ010000218         7345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000218 DAZKFZ010000000
VERSION     DAZKFZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.218"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..402)
                     /locus_tag="ACHOQW_004070"
     CDS             complement(<1..402)
                     /locus_tag="ACHOQW_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9575048.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLM"
     gene            complement(415..2196)
                     /gene="gcl"
                     /locus_tag="ACHOQW_004071"
     CDS             complement(415..2196)
                     /gene="gcl"
                     /locus_tag="ACHOQW_004071"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP9575049.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2286..3101)
                     /gene="allR"
                     /locus_tag="ACHOQW_004072"
     CDS             complement(2286..3101)
                     /gene="allR"
                     /locus_tag="ACHOQW_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP9575050.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3179..3661)
                     /gene="allA"
                     /locus_tag="ACHOQW_004073"
     CDS             complement(3179..3661)
                     /gene="allA"
                     /locus_tag="ACHOQW_004073"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP9575051.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3891..4817
                     /gene="allS"
                     /locus_tag="ACHOQW_004074"
     CDS             3891..4817
                     /gene="allS"
                     /locus_tag="ACHOQW_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP9575052.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            4886..5980
                     /gene="mnmH"
                     /locus_tag="ACHOQW_004075"
     CDS             4886..5980
                     /gene="mnmH"
                     /locus_tag="ACHOQW_004075"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP9575053.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            6096..6461
                     /gene="ylbG"
                     /locus_tag="ACHOQW_004076"
                     /pseudo
     CDS             6096..6461
                     /gene="ylbG"
                     /locus_tag="ACHOQW_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7211..>7345)
                     /locus_tag="ACHOQW_004077"
     CDS             complement(7211..>7345)
                     /locus_tag="ACHOQW_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575054.1"
                     /translation="YINYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFLIFP
                     "
BASE COUNT         1876 a         1914 c         1757 g         1798 t
ORIGIN      
        1 catcagcata ttcgcggcat aacgcaggtt ttctacaagc gttgcgtgaa tctgttcact
       61 gctgaaacca gccggcgttt taccgaccag acagttaata tttttattac ccagcgcacg
      121 ggcataacga atcgctgctg ctacgccatc ccgaaactct tcttcacggc caggaataca
      181 ggcaataccg cgctcccccg ccgcccagtc acccgccggt aaattgtgca gcgtatgttc
      241 gagtttatta ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat aaggaaacat
      301 aaattcaacg ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata
      361 ttcaccaaat aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc
      421 atagtgcatg aagcaggttt cagtcggtgc atccgctgcg ttatcggcga tatcttcaaa
      481 ttccatgacg ttatccagtt cgctgcccat cgaaatattt gtcacacgct cgagaataac
      541 ttccacgact accggtaccc gatattgcgc cattaaggct ttcgcctgtt caaaggccgg
      601 tgcaatatct tccggtttga agacgcgaat agctttacaa cctaaacctt ccgctacttt
      661 tacgtggtcg acgccgtaac cgttcacttc gctggagttg atattctcga aagcgagttg
      721 cacgcagtag tccatgtcaa aggcgcgttg tgactgacga atcaggccga gataggcgtt
      781 attcaccagc acatggatgt acggaatgtt gaactgcgcg ccaacggcta actcttcaat
      841 caggaactgg aagtcgaagt caccggaaat cgccaccaca ttgcgtttcg gatcagcggc
      901 acaaaccccc agcgccgccg gaatcgtcca gcctaacgga ccagcctgac cacagttgat
      961 ccagtggcgg tctttaaaga catgcagcat ttgtgccgca gcgatttgtg acaggccaat
     1021 ggtggtgaca taacaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg
     1081 cggtttcacc ggcacgttgt cgaaatgggt tttgcgcagc aaagtgcgtt tgcgctgctg
     1141 gcaatcggcg acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc
     1201 cacttcaacc agcagtgtca gcgccgcttt agcatcagag acaatgccga gatccggaca
     1261 cagcacgcgg ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt
     1321 gtatttctct accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat
     1381 gtcggacgcc agcaacgttg cgttaccgta acgatgcgcg gtttgcagac cgaccatccc
     1441 ggccatcagt tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg
     1501 aacgctggtc agttcagcaa actgttgtaa cagtgcagct gcgtcagcat tgattacccc
     1561 gcccccggca acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat
     1621 ctgcatacgg ctggcagcag gtttgtagac cggcagcggt tcgtacatgt caggatcaaa
     1681 ctcgatttcc gcaacctgaa cgtcgaacgg taaatccacc agtaccggac ccggacgacc
     1741 agaacgcatc aggtgaaatg cctgttgcag cacgcgaggc accagcgccg cttcacgaac
     1801 tgtaaccgcc attttgctga ccggtttagc aattgcttca atatctacgg cctgaaaatc
     1861 ttctttatgc agacgggcgc gcggtgcctg gccggtaatg cacagaatag gaatggaatc
     1921 agcagaagcg gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag
     1981 acatacgccg atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc
     2041 ttccacatga cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa
     2101 cggattgatt gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac
     2161 atacattgcc gcgtcaacgg ctctcatttt tgccatttta ttcctacctc tattaattgg
     2221 aaaaattttc caactttcaa attttgtcaa aaaaacccgc tccgcagagc gggatgcgac
     2281 agacactatg gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg
     2341 accagctcac cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg
     2401 gagatagcgg caacaacgct acctacatca tcgtaaattg ctgaagctat gcaattcaga
     2461 cctacaacat gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc
     2521 ttcagcaggg tgggcatatc cacaagcgta gttggcgtaa actgctgcaa accggtttgc
     2581 agaatgatgc tcatcaactc ctcttccgcc agcggataaa gcagcgcttt gcccgcaccg
     2641 gaagcatgca gtggcagacg actgcccagt ggcgcgcaca tcctgaccat cgatttacac
     2701 tctaactgac caattaatac cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg
     2761 ccggaaagta acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta
     2821 tggatgtacg ccgcaccgac gttaaagaca cctaatccta tatgccacca gcctaactga
     2881 ctgtcctgat agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag
     2941 agcggcaaat ccagattgag agaaatatcg ctaaccgacg aacttccccc acttttttcc
     3001 agatattgca gaatcgcaat tccccgctct aatgcctgtg cgcccttctg tgccacaggc
     3061 tccgcctgtc ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc
     3121 tacaagtgag cataaacaat gaacaattag tttatcttat gcaagtcggt tgcaggcgtc
     3181 acgcaaaaca gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc
     3241 gatcgatggt cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt
     3301 tacgatggta attcactccc tgttcgccgt tggtaataaa cgcccgcagg gttgacaggt
     3361 ctggtttgtc gtcacctaac gctacgacca ccacaaacac ctcacctttc atcgggataa
     3421 aggcctgagt acccagcgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg
     3481 gttgcgctcg gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca
     3541 aatcgtggta acgctccacc aggccattgt taatatggaa aaaatcccgt tgctgcgttt
     3601 cgattacgtc gccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca
     3661 tctttttgtc cttgtaacat aattccgttc aaccagcgcc gaccggtcag cttggtttca
     3721 ataacaggca atctaaaacc ttaagagaac aacatcaaca caaaacttgg aatatttttc
     3781 caaaactaga tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca
     3841 gaggataaga aaatcacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc
     3901 cagaaacctt gcggactttc attgcggttg ctgaaacagg aagtttttca aaagcggcag
     3961 aacgattatg taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata
     4021 ccggagtggc gctgtttttt cgcacgactc gtagcgtaac gttgacagcg gctggcgagc
     4081 atctactttc ccaggccaga gactggctga gctggctgga aagtatgccc agcgagctgc
     4141 aacaggtgaa tgatggcgtg gaacgccagg tgaatattgt catcaacaac ctgctctaca
     4201 acccccaggc cgtcgcccag ttgctggcgt ggctgaatga acgttacccc tttacccagt
     4261 ttcacatctc ccgacaaatc tatatgggcg tctgggactc gctattgtac gaaggttttt
     4321 cactggctat cggcgtcacg ggaaccgaag cgctggcaaa tacctttagt cttgatccct
     4381 taggatcggt gcaatggcga tttgtcatgg cggcggatca tccgctggcg aacgttgaag
     4441 agccgctaac agaagcgcag ttgcggcgct ttccggcggt caatattgaa gacagcgccc
     4501 gcaccttaac caaacgcgtc gcctggcgat tgccagggca aaaagagatt attgttcctg
     4561 atatggaaac gaaaatcgcc gcccatctgg cgggcgttgg cattggtttt ttgccaaaat
     4621 cgctttgcca gtcaatgatc gataatcaac aactggtcag ccgggtaatc ccaacgatgc
     4681 gccctccttc gccattgagt ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag
     4741 atattgtgac cttgtttacc cagcgcaggc cggaaatcag cggattttta gaaattttcg
     4801 gcaacccacg cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa
     4861 gctgatttga ggttcacttt taactatgca agagagacac acggaacagg actatcgtgc
     4921 cctgctgatt gctgatacgc ccattattga tgttcgcgcc cctatcgagt ttgagcaagg
     4981 cgcaatgccc gccgctatca atctgccgtt aatgaataac gatgaacgcg ccgccgttgg
     5041 cacctgctat aaacagcaag gctcagacgc agcgctggcg ctgggacata aactggttgc
     5101 gggtgaaatt cgtcagcagc gcatggacgc ctggcgggca gcctgcctgc aaaatccgca
     5161 aggtattctc tgctgcgccc gtggcggtca gcgctcacat attgtgcaaa gctggttgca
     5221 tgcagcgggg attgattatc cgctggtgga aggcggttat aaggcactgc gccagaccgc
     5281 gattcaggcg actattgaac tggcacaaaa accgatagtg ctgattggcg gttgtaccgg
     5341 cagcggtaaa acgctgttag tgcagcaaca gccgaacggt gttgatctgg aagggttggc
     5401 gcgtcatcgc ggttcggcgt ttggtcgcac gttacaacca caacttagcc aggcgagttt
     5461 tgaaaacctg ctggctgccg aaatgctaaa aaccgacgcc cgtcagaatt tgcgcctgtg
     5521 ggtgctggaa gacgaaagcc ggatgatcgg ttcgaatcac ctgccggaat gcctgcgcga
     5581 gcgaatgact caggcggcga ttgcggtggt agaagatccg tttgagatcc gtcttgagcg
     5641 cctgaacgaa gagtatttct tgcgtatgca tcatgatttt acccacgcgt acggcgacga
     5701 acagggctgg caggaatatt gcgaatacct gcatcacgga ctttcggcta ttaagcgtcg
     5761 gctggggcta cagcgatata acgaactggc tgcaaggctg gatgcagcac tgacaacgca
     5821 actcaccacc ggcagcaccg acggtcatct ggcctggctg gtgccgttac ttgaagaata
     5881 ttacgacccg atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg
     5941 tgagtgggcg gaagtggcgg aatgggttaa ggcgcagtaa tgagggacgt atttgtatta
     6001 gtcgcgactt gatctgacac atggccttga aaggttgaga gttaccggtt ttgatatgag
     6061 tgtcgaatcc ttatccaaaa caagaggtaa ctctcatgct tcatactgcc aatcccgtca
     6121 tcaaacacaa agccggtttg ctcaatctgg ctgaagaact cagcaacgta tcaaaagcct
     6181 gtaaaatcat gggcgtttcg cgtgatacgt tttatcgtta ccgcgaactg gtcgctgaag
     6241 gcggtgtgga tgcgcagatt aatcgtagtc gccgcgctcc taaccttaag aaccgtaccg
     6301 atgaggcaac tgaacaggct gttgttgatt acgccgtcgc tttcccgaca catggtcagc
     6361 accgggccag caacgaactg cgtaaacagg gggtttttat ctccgacagt ggtgtccgtt
     6421 ctgtctggtt actccataac cttgagaatc tcaaaagacg atactgaaca aattttatca
     6481 ggtgacgttc cgtaaaaagt tgtatggagt tctcgatgca gtcggcgcga ctatcagtac
     6541 ccccgctgaa ccctggacta tcgggccgcc gtactggggc atgtcaccct ggcgtgccgc
     6601 tggttttccg ctaatggttt atttcctctt tctttcccgg caagaattta ttttaatccg
     6661 gaaggattat ttattttcag atagggactc acattaaaca cgtaacattt taattaatgc
     6721 atggctgaat atgtattaca caatgtcaga tatcgtaaaa gaccagtgca attctatgta
     6781 aactctctga ctgttcattt tatttgttgt ttcagggtcg gaactcaccc tcaataaaca
     6841 actgaatacg agtagatatt catcgtctga gctatatggc tttacacaat agccgacatg
     6901 tcttttctca gtcaggaatt ttacttttcc tcactacgaa gttgacgact tcgatatggg
     6961 atagactctt aattcaagca attactctgc acaagcatca ggcaatccag ctccaatatt
     7021 tgttataaaa taatgcgtaa cattaatttc accaacaaaa aaaggtttac accatcccct
     7081 tgccctggta tcttaccata tttacaccac actttttttg tatctggatc attctttgat
     7141 acgattttcg ccacttgttc cctggccctc actgtcaatg ttttcttaaa cctcccccca
     7201 tcatcatttg ttagggaaaa ataagaaaaa aataatcatc ttctacattt gtgtatattg
     7261 atatatccac cgctacgtgc ggaaaaaaat ctgggagcca tgtaaaaact ccatcatttt
     7321 ctggtattgc catatagttt atgta
//
LOCUS       DAZKFZ010000219         7287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000219 DAZKFZ010000000
VERSION     DAZKFZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..562
                     /locus_tag="ACHOQW_004078"
     CDS             <1..562
                     /locus_tag="ACHOQW_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575055.1"
                     /translation="FSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSA
                     NQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSN
                     NQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGAN
                     NWTPTTSISDLLVVVMKKSTAGISIS"
     gene            629..1228
                     /locus_tag="ACHOQW_004079"
     CDS             629..1228
                     /locus_tag="ACHOQW_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9575056.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1293..4208
                     /locus_tag="ACHOQW_004080"
     CDS             1293..4208
                     /locus_tag="ACHOQW_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9575057.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAVSQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHS
                     VSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            4208..4789
                     /locus_tag="ACHOQW_004081"
     CDS             4208..4789
                     /locus_tag="ACHOQW_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9575058.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(4909..5799)
                     /locus_tag="ACHOQW_004082"
     CDS             complement(4909..5799)
                     /locus_tag="ACHOQW_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9575059.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(5818..6324)
                     /locus_tag="ACHOQW_004083"
     CDS             complement(5818..6324)
                     /locus_tag="ACHOQW_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9575060.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(6361..6861)
                     /locus_tag="ACHOQW_004084"
     CDS             complement(6361..6861)
                     /locus_tag="ACHOQW_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9575061.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            complement(6940..7122)
                     /locus_tag="ACHOQW_004085"
     CDS             complement(6940..7122)
                     /locus_tag="ACHOQW_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9575062.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
BASE COUNT         1942 a         1727 c         1809 g         1809 t
ORIGIN      
        1 attttccctg acaccggacg gaaagctgac tgctaaaaat gcggatatca gtggcagtgt
       61 gaatgcgaac tccgggacgc tcaacaacgt cacgattaat gagaactgtc agattaaggg
      121 gaaactgtca gccaatcaga ttgaaggcga tattgtcaaa acggtcagca agtctttccc
      181 ccgcacgagc acttatgcca gtggcaccat cacggtaaga atcagtgatg atcagaaatt
      241 tgaccggcag gtcatgatac cgccagtgtt attccgcggt ggtaagcatg agaatttcaa
      301 cagtaataac caacagtcat actggtattc aacctgccgg ttaagagtga cccgcaatgg
      361 ccaggagatt tttaatcagt ccacgacgga tgctcagggc gtattttcct cagttataga
      421 tatgcctgcc ggacagggga cgctgacact gacattcacc gtatcttcat caggagcgaa
      481 taactggaca ccaacaacca gtatcagcga tctgctggtt gtggtgatga aaaaatccac
      541 agcaggtatc agtatcagct gaattttata acccagaacg ggcgtcagaa atgacgcctt
      601 ttttattgca gaaaagcgag aggtaattat gcgtaaactt tatgccgcca ttttgtccgc
      661 agccatttgt ctggccgtat ccggtgcgcc tgcatgggca tctgaacatc agtccacgct
      721 gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc tgaacgggat
      781 taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt cattcagcta
      841 tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc atgaagattc
      901 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
      961 cagcgcgtat gcgatggcgg gtatggctta cagccgtgtt tcgacttttt ccggggatta
     1021 ccttcgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatgacgg
     1081 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     1141 cgtggccatt gatattgctt atgaaggttc tggcagtggt gactggcgca ctgacgggtt
     1201 catcgtgggt gttggttata aattctgatt agccaggtaa cacagtgtta tgacagcccg
     1261 ccggttcagg cgggcttttt tgtggggtga atatggcagt aaagatttca ggtgtactga
     1321 aagacggcac aggaaaaccg gtacagaact gcacaatcca gctgaaagca aaacgtaaca
     1381 gtaccacggt ggtggtgaac acgctggcct cagaaaatcc ggatgaagcc gggcgttaca
     1441 gcatggacgt tgagtacggt cagtacagcg ttattctgtt ggtggaaggg ttcccgccgt
     1501 cacatgccgg gaccatcacc gtgtatgaag attctcaacc cggtacgctg aatgattttc
     1561 ttggtgccat gactgaggat gatgtccgtc cggaggcact gcgccgtttt gagctgatgg
     1621 tggaagaggt ggcgcgtaac gcgtccgcgg tggcacagaa cacggcagcc gcgaagaagt
     1681 cagccagcga tgccagcaca tcagcccgtg aggcggcaac ccatgcgact gatgctgcgg
     1741 actcagcacg cgcagccagc acgtcagccg gacaggccgc gtcgtcggct cagtcagcgt
     1801 cttccagcgc aggaacggca tcaacaaagg ctactgaagc atcaaaaagt gctgccgctg
     1861 cagagtcctc aaaaagcgcg gcggctacca gtgccggtgc ggcgaaaacg tcagaaacga
     1921 atgcggcagt gtcacaacaa tcagccgcca cttctgcatc caccgcgacc acgaaagcgt
     1981 cagaagctgc ctcctcagcc agggatgcgt cggcttcaaa agaggcggca aaatcatcag
     2041 aaacgagcgc agcctcgagc gccagtagtg cagcctcctc ggcaacggcg gcaggcaatt
     2101 ccgcgaaggc ggccaaaacg tctgagacaa acgctaagtc ctctgaaacg gcagcagaac
     2161 agagtgcctc cgcagcagca ggctcaaaaa cagcggctgc attatctgcc agtgccgcgt
     2221 caacaagtgc cgggcaggcc tcagccagtg ccaccgccgc cggaaaatcg gcagaaagtg
     2281 ccgcatcgtc tgcttcaaca gccacaacga aggctggcga agccactgaa caggccagcg
     2341 cagcagcgag ttctgcttcc gcagcgaaga catccgaaac gaacgcgaaa gcgtcggaaa
     2401 ccagcgcaga atcctcaaaa acggctgccg catcgtcagc cagttcggcg gcgtcatcgg
     2461 catcatctgc gtctgcttca aaagatgagg cgaccagaca agcgtcagca gcgaagagca
     2521 gcgccacgac ggcatccacg aaggcgacag aggcagctgg tagtgcgacg gcagcagctc
     2581 agagcaaaag tacggcggaa tctgcagcaa cgcgcgctga gacagcggca aaacgggcag
     2641 aggatattgc atccgccgtg gcgcttgagg atgcgagcac gacgaaaaag gggatagtac
     2701 agctcagcag tgcgactaac agcacttccg agtcactggc ggcaacgcca aaagccgtta
     2761 aggccgcgta tgagctggct aacgggaaat acaccgcaca ggatgcaacg acagcacaga
     2821 aagggatagt tcagcttagc aacgcgacca acagcacatc tgaaatgctg gcggcaacgc
     2881 caaagtcggt aaaggcagcc tatgaccttg ctaacgggaa atatactgct caggacgcta
     2941 cgacagcaca aaaaggaatt gtccagctca gtagtgcaac caacagcgca tctgaaacgc
     3001 ttgccgcgac accgaaagca gtgaaagcag ctaatgataa tgcgaatggt cgggtacctt
     3061 ctgcccgtaa ggtgaatggt aaggcgcttt catcggatat aacactgacg ccgaaagata
     3121 ttggtacgct taactcaaca acaatgtcat tcagcggtgg tgctggttgg ttcaaattag
     3181 caacggtaac catgccacag gcgagttctg ttgtttcaat tacgttgatt ggtggcgcgg
     3241 gatttaacgt ggggtcacct caacaggcag gtatatctga acttgttttg cgtgcaggta
     3301 atggtaatcc gaaggggatt actggtgctt tatggcagcg cacatcgaca gggtttacaa
     3361 attttgcctg ggtcaataca tctggtgata cttacgatat ttacgttgca atcggaaatt
     3421 atgcgactgg tgtaaatatt caatgggatt ataccagtaa tgccagcgtg acgattcata
     3481 cgtcaccagc atattctgct aataagccgg aagggttaac ggacggtaca gtttattcac
     3541 tctatacgcc atcagagcag ttttatccgc ctggcgcacc aatcccgtgg ccatcagata
     3601 ccgttccgtc tggctatgcc ctgatgcagg ggcagacttt tgacaaatct gcatacccga
     3661 aacttgcagc cgcttatccg tcaggcgtga tccctgatat gcgtggctgg acgattaagg
     3721 gcaaacctgc cagtggtcgg gccgtattgt ctcaggaaca ggacggcatt aaatcgcaca
     3781 cccacagcgc cagcgcatcc agtacggatt tggggacgaa aaccacatcg tcgtttgatt
     3841 acggcactaa atccacgaat aacaccgggg cgcacacgca cagtgtgagc ggtacagccg
     3901 caagtgccgg aaaccatact catagtgtca caggcgcatc agcagtcagc cagtggtcac
     3961 aaaatgggtc agtacataag gtagtgtctg cggccagtgt gaatacaagt gctgcaggag
     4021 cgcacactca tagtgtcagc ggcacagctg catctgcagg tgctcacgca catactgtcg
     4081 gtattggtgc tcatacgcac tctgttgcga ttggctcaca tggacacacc atcaccgtta
     4141 acgctgcggg taacgcggaa aacactgtca aaaacatcgc atttaactac attgtgaggc
     4201 ttgcataatg gcattcagaa tgagtgaaca accacggacc ataaaaattt ataatctgct
     4261 gaccggaact aatgaattta ttggtgaagg tgacgcatat attccgcctc atacaggtct
     4321 gccagcaaac agtacctata ttgcaccgcc agatattccg gctggcttcg tggctgtttt
     4381 caacagtgat gagtcatcgt ggcatctcgt tgaagatcat cggggtaaaa cggtttatga
     4441 cgtggcttcc ggcgacgcgt tatttatttc tgaactcggc tcattaccgg aaaatgttac
     4501 ctggttatcg ccggaagggg agtttcagaa gtggaacggc acagcctggg tgaaagatgc
     4561 agaagcagaa aaactgttcc ggatccggga ggcggaagaa acaaaaaaca gcctgatgca
     4621 ggtagccagt gagcatattg cgccacttca ggatgctgta gatctggaaa tcgcaacgga
     4681 ggaagaaacc tcattgctgg aagcctggaa aaagtatcgg gtgttgctga accgtgttga
     4741 tacatcaact gcacctgata ttgagtggcc tgtaatacct gaagtttaaa ataaaagccc
     4801 gcttaaaata tcgcgggctt cgaaatataa atgtattttc agaggctaga gcttaggata
     4861 tctcagcggc aattaactta tgagggatag ctaaatacca gaagataatt agtttttact
     4921 atttatcttt gtttgtggtt ctccttcagc aagctcagcg ccagtgacag gattgatgtc
     4981 ttcatgggat ttcaaccttg ttgccatagc ttttatcata gcttcttgct tggtcgaaag
     5041 atttatcgag gccttcccat ccccaccgtc aaccgctgaa actggatcgg aaacatagtc
     5101 aaagttttca tcactgttcc aactacctct gacgtcttca ccttctgaca tgttgtaata
     5161 tacgtttttg tatttctcaa gtggtggtaa ttttcctggt ggaaaggtgt tacgaattga
     5221 atgtaatgct ttttcaaatg aaagcatgtg tgctgcttcc cgggtcatta aaaatgccag
     5281 agtgtctttt actccaggat catcagtaac attaatgaga cgttcgtaaa tgatctttgc
     5341 ccgagcttca gctgcaatat ttgaacgaag atcggccgtg acttcgccaa tagtatcaac
     5401 ataagctgca gtccagggta ctccagctga attagttaat gctgggcctc ctccgtagag
     5461 gagagaagtt atatggctat cattgccatt ttgagttaag gaacggtaaa gctcagcttc
     5521 attctcagtt ccttcagcaa gtgctccttt tgcacctttg ttgagcatac caacaagaga
     5581 accaataatt tcgagatgac ttagttcttc tgttgctatg tccatcagca tatcccttcg
     5641 gcctgcatct tcatcactta agccttgagt gaagtatcgg catgctgctg caagctcacc
     5701 ctgtggccca ccgaattgtt ctaagagtag attagccaag cctgggtttg gctcacttac
     5761 acgtactgta tattgaagtt ttttcacgtg tctaaacata atgcctcctc aaaagaatta
     5821 ttttttggct tcaactccat caatttcaga tctgaccatg aattgctcag tggtgtcagg
     5881 aatatggtta agaagccatt cagccatttg tttttcttca ttaagaattg cttcaataat
     5941 gggtactgaa gctgtatcac ccgcattttt ggctgctgcg agaagagatg tgtaacacgc
     6001 aatttcaaat tgttcgaaca catagccact gatcgaacct ttgacaattt catcagatgg
     6061 aaaaattcca ccaatggatt gtccaaacgc ggccatttta ctcatagagt ctttaatgac
     6121 agagcgcgaa atattattac gatcaagtat gctttcaagc tgtgaaagct ggttctttgt
     6181 ttcgctaatg tgctgctcaa ttctggaacg taactcagga tagttttcta tccggctggc
     6241 catagactca agcatttttt cagcttgttt ttccatcgca tgtgcatcac gaagccagtc
     6301 atggtaatgt tcaattctat tcataatctg tttatcctaa aagaaattga agtctatagt
     6361 tcaggagtta tcctgagctt ttttattaac gttattaaat gccaggtctg ttaatttgac
     6421 atcagtagct ttttcctctt ccagtgtttc tttcaggagt ttagccgctt ttttatatcc
     6481 taattgctca gcgagtgtta ccaaagtgcc ataactggca atttcatagt gctctacttt
     6541 ttgtgctgca gcaatcaatg cagcatcacg tacttcattt ttatcagtgc tttcaattac
     6601 ctcattcgcc tcttcgataa gtccttccat tgcggcacac ttaattcttt tcaatttaag
     6661 accatcttca gaatcaacaa cctgatcgat gcgttcaatt tgaccatgtg tctcatcaag
     6721 atgtgactga aaagcagcgg ttaatttatc actatatgca gagcgactaa gtttagacag
     6781 tgcctttgtt agttgtttct cagcgctgta tgtatccgat aataagtgga tgaagatatc
     6841 ttcaacagat ttaatttgca taatcactcc agagttttca ctgcgagccc gtaggctcgc
     6901 agttagcttc agttattcac gaatgtaggg ggtgataaat taagagtcag atttacggcc
     6961 gccaccatgg ctattctgac cacctttttt ccctgcttct gatgctcttt gtggatcgtt
     7021 tttaaaatta ccgccactat gttggccgcc tttacgacca gcgtcagaag ctttttgtcg
     7081 atcttcagcg aaattacctg caccgccacg atgttcggtc atatcatttc tcctctgatt
     7141 ttcaaattgt aaataacatg tgccttgtca gcacccaatt aactctagta acaaataaga
     7201 aagtgtcaaa taagagattg gtatgcgcat cttaatgtga gaaatagcaa ttgtaaaaat
     7261 gtgtactcag tgagtataat ctgttga
//
LOCUS       DAZKFZ010000220         7274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000220 DAZKFZ010000000
VERSION     DAZKFZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..95
                     /locus_tag="ACHOQW_004086"
     CDS             <1..95
                     /locus_tag="ACHOQW_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575063.1"
                     /translation="RSSLKENTETVEMNSKVWGRLKKIITFFKK"
     gene            complement(171..473)
                     /gene="bamE"
                     /locus_tag="ACHOQW_004087"
     CDS             complement(171..473)
                     /gene="bamE"
                     /locus_tag="ACHOQW_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9575064.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
     gene            complement(524..1249)
                     /locus_tag="ACHOQW_004088"
                     /pseudo
     CDS             complement(524..1249)
                     /locus_tag="ACHOQW_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            complement(1191..1385)
                     /locus_tag="ACHOQW_004089"
     CDS             complement(1191..1385)
                     /locus_tag="ACHOQW_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575065.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRFGTMEERNRLQ"
     gene            complement(1406..1687)
                     /locus_tag="ACHOQW_004090"
     CDS             complement(1406..1687)
                     /locus_tag="ACHOQW_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575066.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(2226..2312)
                     /locus_tag="ACHOQW_004091"
     tRNA            complement(2226..2312)
                     /locus_tag="ACHOQW_004091"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2276..2278),aa:Leu,seq:taa)
     gene            complement(2325..2398)
                     /locus_tag="ACHOQW_004092"
     tRNA            complement(2325..2398)
                     /locus_tag="ACHOQW_004092"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2364..2366),aa:Cys,seq:gca)
     gene            complement(2452..2527)
                     /locus_tag="ACHOQW_004093"
     tRNA            complement(2452..2527)
                     /locus_tag="ACHOQW_004093"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2492..2494),aa:Gly,seq:gcc)
     gene            complement(2679..3227)
                     /gene="pgsA"
                     /locus_tag="ACHOQW_004094"
     CDS             complement(2679..3227)
                     /gene="pgsA"
                     /locus_tag="ACHOQW_004094"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9575067.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(3284..5116)
                     /gene="uvrC"
                     /locus_tag="ACHOQW_004095"
     CDS             complement(3284..5116)
                     /gene="uvrC"
                     /locus_tag="ACHOQW_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9575068.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(5113..5769)
                     /gene="uvrY"
                     /locus_tag="ACHOQW_004096"
     CDS             complement(5113..5769)
                     /gene="uvrY"
                     /locus_tag="ACHOQW_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP9575069.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            5911..6027
                     /locus_tag="ACHOQW_004097"
     CDS             5911..6027
                     /locus_tag="ACHOQW_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9575070.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            6065..6241
                     /gene="yecU"
                     /locus_tag="ACHOQW_004098"
     CDS             6065..6241
                     /gene="yecU"
                     /locus_tag="ACHOQW_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP9575071.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            6228..6452
                     /gene="yecF"
                     /locus_tag="ACHOQW_004099"
     CDS             6228..6452
                     /gene="yecF"
                     /locus_tag="ACHOQW_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP9575072.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(6520..7242)
                     /gene="sdiA"
                     /locus_tag="ACHOQW_004100"
     CDS             complement(6520..7242)
                     /gene="sdiA"
                     /locus_tag="ACHOQW_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP9575073.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         1918 a         1830 c         1612 g         1914 t
ORIGIN      
        1 taagatcctc tttaaaggaa aatactgaga ctgtagaaat gaacagtaaa gtttggggga
       61 ggctgaaaaa gataatcact ttctttaaaa agtgatttat gtttctgtaa aaagcccgta
      121 ggtatcaact gcgggctaaa ttttcccttg atgctggttt tgtcttttat ctaaaaaatg
      181 ccagtcttca cgttgcttgt tccagcatta aagatgtatg tgctgacttt ttcgcctttg
      241 aaagaaactg tcagagattt agtttgtgag tctgcgccac ctgcaagcag tcccacaacg
      301 gggatgaaag aggttgcttt ggactggctg ttatacatag tgtatgacca ttgttcttca
      361 ccatcgatca aagaacggct gtcaggttca ccgaacgatg ctaacacgtc ctgttttgta
      421 gttttgcctt taacaatttt ggttttcaca ctttcctgcg tctcattttt caatgactgg
      481 ttgcctactg aagaacaacc tgccaaggtc agaaaaacga atctatatga gagttttttg
      541 tacctgagac taaatcgccc tcccaatgcc ctagagagcg tctgttatcg atatttcggg
      601 aacgttcgtg aattggtgtt ccgttcacta tgttaatcgt acctctttcg cctttgcggg
      661 tatgacgcct gccatggcga aggctatgcg accgtcgcag atgctgtata ttcaggtggt
      721 gtagcgcttc acggctacga aagtacagcg ttttataaat tgtctcaggt gatattcgca
      781 gcgttttttg acgtggtttt gttcgcctta accatcctga tatttgctct ggagaccatt
      841 tcatctccag cttttccaga acaagctttc gcaatggtaa attttgatcc agtaagcacg
      901 gttttggcct tttcgccatt ctgttggctc ggttattagc atcaacagct ttgtaatagc
      961 gtctgccccg attacgctga acttcacgtg agatcgtcga aggactgcga ttcagcgcag
     1021 tagctatcgc acgaatgctc attttggctg acaaaccagc tcgtatctcc tcgcgctcag
     1081 acagtgtcag gtgagctaca gcccgcttac gctcatgggg ttttatgccg ccagtatccc
     1141 ttaacatagt gaagatcgtt ccgggttttg aacccaggat attcgctatt tcactgaagc
     1201 ctgttccgtt cttccatagt tccaaacctc cgcaacccca tgttttcaca taactgttgc
     1261 gttgaccaat tgaatctaca cttacattgt tgatgaaatc gctcacgaag gaccaccgga
     1321 caggctttca gccagaagcg cagattttcg ggatgtgaac cgttaacggc acataatgct
     1381 cgcacaacaa acctgttaaa agagattact cagcattctc ctgcttccat tgccggatca
     1441 tttcatcggt aacatcaccc tcataacata ccacgtcata ccctccatta cggctatatg
     1501 cactgcgtcc accacatcta ctaccattcc ttgcatgatt ataaggacac gcacaattgc
     1561 ctggataaga ttcaatggat tctttaatta tttctttttt gatctgagca tcagactttc
     1621 ctgttgctgc atatccacta aaagatacta aacaaaggca gattgccatt aataaaccac
     1681 atcgcatacc gatacactca attactaacc aatcatagcc cacatcataa aatcgagatg
     1741 aggagcacaa tacctttaac aagaaggtgt cataacgata atatgtcgtg caattcatca
     1801 aaaaaaagat ctatatcaaa gagatagaac tcttatcagt gcttcagaca tcggcattcg
     1861 aggcagcaga aaaacagtaa atctgacgaa tttgcctttt cccaagatgt tgtcagatta
     1921 aaaagataga ttagcgcagg tacctgttgg tggattgcag gaggaaaaat aaagtcgagt
     1981 ggaaaaatcc cagaaaaatt tttgtttaac agaggtcctg gttcactatg cgaaatacgg
     2041 aggagctgca gaagaattct gcttcatcct tagagtttaa acagcacgtg cactaccatt
     2101 gtttctgcat atcgccacac catcgccact ctaccgccat tgcacaaaat acaaatacga
     2161 aaaaaccacc cgaaggtggt ttcacgacac tgcttattgc tttgatttta ttcttagctt
     2221 tcccatggta cccggagcgg gacttgaacc cgcacagcgc gaacgccgag ggattttaaa
     2281 tcccttgtgt ctaccgattc caccatccgg gctcgggaag aaagtggagg cgcgttccgg
     2341 agtcgaaccg gactagacgg atttgcaatc cgctacataa ccgctttgtt aacgcgccaa
     2401 attcttcagg cctttcagcc agacatccgc tgacgccgat gtcttttaaa ctggagcggg
     2461 aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta
     2521 ttcccgcatt catcaagcaa tcagttaatc acttgatttt attatcgtct ggcaatcagt
     2581 gccgccgttc gatgcgttgc attctactta cctggcgcga tgagtcaacg atatttttca
     2641 ccacttttga tcgtttgctg aaaattgcgc cgaaacgatc actgatcaag caaatctgca
     2701 cgcgcagcgc tcaaatattg caacattgac cacagagtca gtaccgcagc cacaaagaaa
     2761 agtgcaatac cggcgtactc aacccaaatg ttcggacgcc acagcagcca tgccaacgcc
     2821 accatctggg cagtggtttt cactttccca atccaggaga cagccacgct actgcgttta
     2881 cccaactccg ccatccattc gcgtagcgca gaaataataa tttcacgggc gatcatcgtt
     2941 gccgccggta aggtcaccca ccagctgtgg taatgctcgg ttaccagcac catggcgata
     3001 gccacgagaa ctttatctgc tacagggtca aggaaagcac caaaccgggt actctggttc
     3061 cagcggcgtg ccagaaaacc atcgaaccag tcagtcaccg ccgcgacgca gaaaatgagc
     3121 gcggcggcaa acggcgacca agtgacaggc agataaaaga ccaatacaaa gaatgggata
     3181 aggatgacac ggaacagtgt aagcaacgta gggatattaa attgcataat gacgggtaac
     3241 tatctgttgt cagtaagatt acccctatgt tgctacagag acatcaatgt ttcaacgacc
     3301 agaagatctt ttctgccaga ccttgcgaaa tacccggcac ttttgcaatt tcctcgacgc
     3361 tggcgttacg taaaccttgc aaaccgccca tatatttcaa caacatttgc cggcgttttg
     3421 gcccgacgcc ttcaatggtt tccagggaac tggtattttt gaccttcgcc cgttttttac
     3481 ggtgcccgcc aatcgcgtga tcgtgtgatt catcgcgaat atgctggata acatgcagcg
     3541 cgggagaatc tggcggcaaa ctgaatccct caccttccgg ctcaaagaac agcgtttcca
     3601 gcccagcctt acgatctgct cctttggcaa caccaagtag cagcggatga tttttatccc
     3661 atgagacatc cagttcggcg aagacatttt tcgcctgcgc aagctggcct ttgccgccgt
     3721 cgataagtat cacatccggg atcttactgt cgtcgatggc tttaccataa cgccgacgca
     3781 gcacctgatt catcgccgca taatcatcgc ccggcgtgat gccagtaatg ttatagcgcc
     3841 gatactccgc acgcagcggg ccgttagcat caaacaccac acaggaagcg acggtttgtt
     3901 cacccatggt atggctgatg tcaaagcact ccatccgctt cacttccggc aatttcaaca
     3961 cgctggcaag tgctgtaagc cgctggtgaa cggtagattg ctgcgaaagt ttgctggtta
     4021 aggccgtcgc cgcattggtg cgcgcgagtt tcaggtaacg cgccctatcg ccacgaggtt
     4081 tggtttgaac attaatcttg cgtcccgcca gttctgaaag ggaatcggcg agcagcgttt
     4141 tatcgctaag attaaaatcg agcaggatct cacccggtaa ggtgcgcatc tggctgcctt
     4201 gtaaatagaa ctggcctacg aaggtttcta ccacctcgct cagttccgta ccgccaggca
     4261 ctttcgggaa atagctgcgg ctgccgagca ctttgccctg acgaatgaac aatacgtgga
     4321 cacaagccat acccgcatcg aacgccacac caataacgtc gaggtcgtcg ccggtattgg
     4381 aaacgaattg tttttcggtg acgcgtcgca ccgcctgaat ttggtcgcga atacgtgccg
     4441 cttcttcaaa ctccagattc tgactggcag tttccatacg gctaatgagt tgcgtaagca
     4501 cctgatcatc tttgccagac aaaaacaggc gcacatactc gacctgctga gcgtattctt
     4561 cttcactcac cagtccttca acgcacggtc ccagacagcg ccctatctga tattgcagac
     4621 acggacgcga gcgattgcga taaacactat tttcgcactg gcgaatgggg aaaatctttt
     4681 gcagtagcgc cagtgtttca cgtacggcat agccattcgg gaacgggccg aaatattcac
     4741 ctttggcatg ctttgcacca cgatgcatcg ccagacgcgg gtgggtatcg ccactcagga
     4801 agataaaagg atatgattta tcatcgcgta gcaaaacgtt gtaacgcggc tgatagagtt
     4861 tgatgtagtt gtgttccagc aacagcgctt cggtttctgt atgagtaacc gttacatcaa
     4921 tttgctggat ctgggcgacc agcgcttcgg ttttgcgcga agcgaggttg ctacggaaat
     4981 agctggaaag ccgttttttc aggtctttcg ctttgccgac atagataacc gtaccaccag
     5041 catcgtacat gcgataaacg ccaggctggc tggttacggt ttttaaaaac gcttttgcgt
     5101 caaactgatc actcactgac ttgataatgt ctccgcatta cacagaccat ggcgaattgc
     5161 caggtgagtc agctcaacat cgccatgaat gtttagttta ctgaacatac gatagcggta
     5221 gctgttcacc gttttcggac tgagattgag ctgttctgag atctcattga ccttctggcc
     5281 cttcgtgatc atcagcataa tctgcaattc acgttcagac aaactggcaa atgggctttc
     5341 tgttttttct ggttcgatct ggcttaatgc catttgttga gcgatgtcag aagcaatgta
     5401 acgctgccca gaatagacag aacggatcgc actcacgact tcctgcggag ccgcgccttt
     5461 gctgaggtag cccgcagcac cggcctgcat gactttcgct ggtaaagggt tttctgtatg
     5521 gacggtaagc atgatgattt ttacatcagc tgtggaacgc gcgattttac gcgtcgcctc
     5581 aagaccgcca atgcccggca tactcatgtc cattagcacc acgtcaacgg catttgcccg
     5641 gcaccactta acggcgtctt caccgcacga tgcctcaccg acgactttta taccctttat
     5701 atcttccaga atgcgtcgta tccctgcgcg caccagttcg tggtcatcaa caagtagaac
     5761 gttgatcaaa ggaatatctc cagaaatagg gataacgcta ctgatagtta gtcattcgta
     5821 tattagcggt ttttattgca actttgaaac gttaaaaatg gtgggtattc gatttttctc
     5881 tcgtttttgt caattcgatt gtccacagtt atggtcacgc ccgtcaccaa cggcttaccg
     5941 gatcgtttca cagtaagccc tgcgacgaaa tgtgacaaaa attaccttta ttcagcaaaa
     6001 atgaaaatca gcccgctgaa tatgtaacat taattaaccc gaacaatacg ggcaaaaaac
     6061 attcatgata aaaatattta ttggtcatta tattaacgca tttaatagca ccgttgacgg
     6121 cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact ccgccgcctt
     6181 aacatttttc accgcaaatt ttcattgcaa catgacgagg aaaataaatg agtacgcctg
     6241 atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc ctgaaagcga
     6301 tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg atgttaaaga
     6361 tggaacaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt tccaaaacgg
     6421 ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgatag cgtgcctttc
     6481 agccggtttt tgcatctggc acgcaggaca gaaaagagat caaattaagc cagtagcggc
     6541 cgcgtaacag gcaacctggg tcttatttgg tgcattaatt tttttctgca tgtttttctg
     6601 gtggaaattg accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt
     6661 cccttccgcc gtccacctca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt
     6721 catcactatt tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg
     6781 catttttaat tgcagttcat cactaagaat gggtatttcg cgcgcgctgc aacgggaaaa
     6841 ggacaaaaag cccagcgccc ggtttggcag cattaaatac tgagtgacac cgcggcgtaa
     6901 accatgtgcg cgcgcggctt cccataacgg ctgtgcttcg ttgaataagt catcattcca
     6961 cattagatgg ccctgactaa agttttcagg gttcagcacc ggatcaattg cgagaaagtt
     7021 ttttgcctga taataactaa cccacgcctc agggtaattg gtgtagaaag ccactttagg
     7081 tcgagtgaat ggtaccgggt ggcggacaca taacgaatag taatcgtact ccagctgctg
     7141 agcctgaagc tcaatttcat ggtagacctc ttctgcggtc tccatcctct gaaaacgcaa
     7201 cagcatcgtg cgacgccagc tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc
     7261 tgagagtgag tatc
//
LOCUS       DAZKFZ010000221         7268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000221 DAZKFZ010000000
VERSION     DAZKFZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            126..1459
                     /gene="adeQ"
                     /locus_tag="ACHOQW_004101"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             126..1459
                     /gene="adeQ"
                     /locus_tag="ACHOQW_004101"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1512..1964
                     /gene="yicN"
                     /locus_tag="ACHOQW_004102"
     CDS             1512..1964
                     /gene="yicN"
                     /locus_tag="ACHOQW_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP9575074.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2080..2403)
                     /locus_tag="ACHOQW_004103"
     CDS             complement(2080..2403)
                     /locus_tag="ACHOQW_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575075.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2387..2740)
                     /locus_tag="ACHOQW_004104"
     CDS             complement(2387..2740)
                     /locus_tag="ACHOQW_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9575076.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2974..4164
                     /gene="nepI"
                     /locus_tag="ACHOQW_004105"
     CDS             2974..4164
                     /gene="nepI"
                     /locus_tag="ACHOQW_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP9575077.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4205..4498)
                     /gene="yicS"
                     /locus_tag="ACHOQW_004106"
     CDS             complement(4205..4498)
                     /gene="yicS"
                     /locus_tag="ACHOQW_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP9575078.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4720..5538
                     /gene="nlpA"
                     /locus_tag="ACHOQW_004107"
     CDS             4720..5538
                     /gene="nlpA"
                     /locus_tag="ACHOQW_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP9575079.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5542..6465)
                     /gene="yicL"
                     /locus_tag="ACHOQW_004108"
     CDS             complement(5542..6465)
                     /gene="yicL"
                     /locus_tag="ACHOQW_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP9575080.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6576..6749)
                     /locus_tag="ACHOQW_004109"
                     /pseudo
     CDS             complement(6576..6749)
                     /locus_tag="ACHOQW_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6929..7034
                     /locus_tag="ACHOQW_004110"
                     /pseudo
     CDS             6929..7034
                     /locus_tag="ACHOQW_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            7045..7110
                     /locus_tag="ACHOQW_004111"
                     /pseudo
     CDS             7045..7110
                     /locus_tag="ACHOQW_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
BASE COUNT         1785 a         1608 c         1813 g         2062 t
ORIGIN      
        1 aatgtcgcaa gattattaaa gaatatccat taatgtgcaa ttgaaatgtg ataattatca
       61 ctgaaccgtt gttgtcattt gttttacaaa agcaagggat taaatctcct ccaatggaca
      121 aaaaaatgaa taatgacaat accgattacg tgagtaatga atcagggacg ctttcgcgat
      181 tatttaaact acctcagcat gggaccaccg tccgcacaga attgattgcg gggatgacca
      241 cttttttaac catggtgtac atcgtttttg tgaacccgca aatcctcggc gcggcacaaa
      301 tggacccgaa agtggtgttt gttaccacct gtttgattgc cggtatcggc agtattgcga
      361 tggggatatt tgctaactta cccgtggcgc tggctccggc aatggggctg aacgccttct
      421 ttgcgttcgt ggtcgtgggg gcgatgggta tctcctggca gaccgggatg ggcgcgatat
      481 tctggggcgc aattgggcta tttttgctca cgctatttcg tatccggtac tggatgatct
      541 ccaacattcc attaagttta cgtattggta tcaccagcgg aatcggatta tttatcgcct
      601 taatgggatt aaaaaatact ggcgttattg tcgccaataa agacactctg gtgatgattg
      661 gcgatttaag ttctcacggc gtgttgttag gtattttagg gttttttatt ataaccgtgt
      721 tgtcatcacg tcattttcat gccgcggtgc ttgtttctat tgtggtgacg tcttgctgtg
      781 gattattttt cggtgatgtt cattttagcg gcgtctattc cattccgcct gatattagcg
      841 gcgtcattgg tgaagtagat ttgagcggcg cgttaacact tgaactcgcc ggtatcattt
      901 tctcctttat gctgatcaac ctatttgatt catcaggaac attaattggt gtaactgata
      961 aagctggctt aatagatagt aacggtaaat tccccaatat gaataaggcg ctatatgttg
     1021 atagcgtagt tcggtggcgg gcgcgtttat cggcacctcg tctgttaccg cctatattga
     1081 aagtacttct ggtgtggcag tcggtggtcg cacggggctg actgcggttg tggtcggcgt
     1141 tatgttcctg ttggttatgt tcttctcacc gctggtggcg atggttcctc cttacgcaac
     1201 cgccggagcg ttaatctttg ttggcgtgtt gatgacctca agtctggcgc gcgttaactg
     1261 ggatgatttt accgaatcgg tgcctgcgtt tgttaccacg gtgatgatgc cctttacttt
     1321 ctcgatcacc gaagggattg cacttggctt tatgtcgtac tgcatcatga aagtgtgcac
     1381 cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc gcagctctgt ttgcactgaa
     1441 gattattctg gtggattagt aactggcgag ggtttccaga tatcatgagt tctgattacg
     1501 caggagaact catgatctgg ataatgctcg ccacgctggc ggtagtgttt gtggttggtt
     1561 ttcgggtgct gacatccggg gccagaaaag cgattcgccg tctcagcgat cggctgaata
     1621 tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg aaagtcagcc ggtgacgaat
     1681 ttttacgtta tttgcatcgt ccggatgagt cgcacctgca aaacgccgcg caggtgttgc
     1741 tcatctggca aattgtcatt gtcgatggta gcgaacagaa cctgctgcaa tggcatcgga
     1801 ttttacaaaa agcccgcctt gccgctccca ttactgatgc tcaggtcagg ctggcgctgg
     1861 gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa tgcttttcag atgcgctata
     1921 acgcgttctt tcagcctgcc gagggcgttc actggctgca ttaaaaccgc ttcgtcttat
     1981 cagcctacag accgcgcctg acctgtaggg tggataaggc gttcacgccg catccggtat
     2041 cggaactgac agtaggtgaa cgcattatct acgttctatt tacagcagca cctccaaccc
     2101 gtgtttttgc acaatgctga gcagttttaa tgaaatgcca ttggggcgtt tagcgccact
     2161 ttcccatttt tgtacagttg aaacgctggt attgagatag tgagcgaaga cgggctgact
     2221 cacttttaat ttttcccgca gtgcttttat ctcaacaggt tgtaagtctt gcacgctggt
     2281 caggcaggct ttgtcaaaag tacgcatcgt ttcagcagaa atagcattga cgctggacaa
     2341 tccagccgct gcgctgtgaa tagcctcaaa tgcaggactt ttaaatttag tttttgcagt
     2401 catagcagat ctctgtcagt tctttagtgt taatcattgc tgttagttgg gctttcgtca
     2461 ggaaggcata atgctttgca atttcacgaa acccagccag ttcctgacta ttgatgttcg
     2521 acatatcctg ttttgcatat aaaaacgtgt aaaaccaatt gcttacccct ttcgccagaa
     2581 taattgcacg ctcacgattt tggtgaaggc gctttttaaa aacgccaccg ccaagtgcat
     2641 cagctttacc ttgttctgtt tctaaaatgg cccggcacaa ctcttcatct gttattgcat
     2701 gagacctggc ttcccttgca aaccatttgg ttttaaacac gcgtatatcg ttgaggtcct
     2761 gttattaaag tatatcacca agtgcaatac tttatccctt gttcccaaca caatcgcgaa
     2821 aatacatatc accttgtcct atgattttta tttcttcgag cagcctcgag cttttcccac
     2881 aacaccgtaa aagttgcaaa aacgttaaat gcgtcacaca tttcaatgtt aaagtggtcg
     2941 gcttttcccc tgaaacatgc cacgggtaac accatgagtg aatttattgc cgaaaaccgc
     3001 ggcgcggatg ccatcacccg accgaactgg tcagccgttt tctcggtggc gttttgcgtc
     3061 gcctgtctga ttatcgttga gtttttgcct gtcagtttgt tgacgccaat ggcccaggat
     3121 ttaggcattt cggaaggggt tgccgggcaa tcggtgaccg tgaccgcctt tgtggcaatg
     3181 tttgccagtt tgtttattac ccagacaatt caggccactg accgccgcta cgttgttatt
     3241 ttgtttgccg ttttgctgac gctctcctgc ttgctggttt cctttgctaa ctcattcagt
     3301 ttgcttttaa tcggtcgtgc ctgtctgggg ctggcgctgg gcgggttctg ggcgatgtcg
     3361 gcgtcgctga ccatgcgtct ggtgccgccg cgtacggtgc cgaaggcgct gtcggtgatc
     3421 ttcggcgcgg tttctattgc gctggtgatt gccgcgccgt tgggctgttt tttaggcgag
     3481 cttatcggtt ggcgcaatgt ctttaatgcg gcggcggcga tgggtgtgct gtgtattttc
     3541 tggattatca aatcactgcc ttcgctgcca ggtgaacctt cgcatcagaa acaaaatact
     3601 ttccgcttat tacaacgtcc gggagtgatg gccgggatga tcgccatctt tatgtccttt
     3661 gccggacagt ttgctttttt cacttatatt cgcccagtgt atatgactct ggcgggattc
     3721 ggtgttgatg gcttaaccct ggtgctgctg agttttggta tcgccagctt tgtcgggaca
     3781 tcgctttcgt cgtttattct caaacgttcg gtgaaactgg ccttagcggg cgcaccgttt
     3841 gtgctcgccc tgagcgcgct ggtactgacg ctgtggggaa gcgataaaat tgttgctact
     3901 ggtgtggcga ttatctgggg gctgaccttt gctttaattc ccgttggttg gtcaacgtgg
     3961 atcactcgct cattggccga tcaggcagaa aaggctggat ctattcaggt tgccgttatt
     4021 cagcttgcca atacctgcgg tgcggcaatc ggcggttatg cgttggataa cattggtctg
     4081 acttcgccgc tgatgttgtc cggcacattg atgttgctga ctgcattgtt ggtgactgca
     4141 aaggtgaaaa tgaagaaatc ctgatatcag gtaaagaagc gttggtcagt ggttgttgcc
     4201 gttcttacat atccgggcat tctaccttac ccaacgcttc ccaataattg ctctgattat
     4261 tacgttccat cgccacgatg gcctcgcgta tatgttgttt attgttttca tctgacaaca
     4321 ttaacttttc ccacgcttca tctgataatg acgcctgtgg cgtgcaaatt ttacgtaaat
     4381 cttgcaatac cgtggttttc tcttttgccg actgtagaga actgaatggc gactcggcct
     4441 gagaaagggc tggaaaaatc aggaatacgg taatcatgag tagcatcgtt ggcttcatag
     4501 cggcaacctt ctgcaaatgt gcatcgaatg aagcataagt gtagtttgct tttgccgctc
     4561 tcatcatgct gtacgaaagg tgtttgcgtg gtcagtaaga agtgccagac tttatattcc
     4621 acttttattc ctttttattc ttatcgatag cgttttgttt tttaaactgc agcgacctta
     4681 ccgctatagt ccggtaatca ttaataaaag gataaaaaaa tgaaactgac aacacatcat
     4741 ctacgggcgg gggccgcatt attactggca ggagttctgc tggcaggttg cgaccagagc
     4801 agcagcgatg aaaagcatat taaagttggc gttattaatg gcgcagaaca agatgtcgca
     4861 gaagtcgcta aaaaggtagc aaaagagaaa tatggcctcg atgttgaact ggtaggattt
     4921 agcggttcgt tgctaccgaa cgatgcgaca aataatggcg agcttgatgc gaacgtcttc
     4981 cagcatcgac cctttcttga acaagataat cagacgcatg gttataaact ggtagcggtg
     5041 ggaaatacgt ttgtcttccc tatggcgggc tattccaaaa aaatcaaaac cgtggcgcaa
     5101 ataaaagaag gtgcgacagt ggcgatcccc aacgacccga ctaaccttgg tcgcgcgctt
     5161 ttactgctgc aaaaagagaa gctaattact ctgaaagagg gaaaagggtt attacctacc
     5221 gcgttggata taaccgataa tccacgccat ttgcagataa tggagctgga aggggcacag
     5281 ttgccacgtg tactggacga cccgaaagtg gatgtagcga ttatcagcac cacttacatc
     5341 gagcaaacag gactttctcc ggttaacgac agcgtattta ttgaagataa aaattcgccg
     5401 tatgtgaata ttctggtggc acgggaagat aataagaatg cagaaaacgt gaaggaattt
     5461 ctgcaatctt atcaatcacc cgaagtcgct aaagcggcag aaaccatctt taacggtggt
     5521 gcggtgcctg gctggtaatt ctcactcatg ccgcgccgga cgatttattt ttctggcgcg
     5581 gcggcgggaa tccattgaaa tcaaaatcac cgatgacaga atcagcagcg ttcccagcca
     5641 gtccggcaat gtgaaggtga tccccagcaa cagcaaagag agcagcgcgc tacttaatgg
     5701 ttcagcacag cttaaaatgc tggcttttgg accgccaatt aattgtgctc ctttcaggta
     5761 cagactaaat gtcagggacg taccaatgac caccaaataa aaaaacgcca gaatcaaact
     5821 tccgttaacg ataaagtttg tcccttgtcc ggcataaaag ggcagcagga tcagaccgcc
     5881 aatgagcata ctccagccga cgaccggtaa cgtgccatag cgggcaatca gcgttgaggg
     5941 ataggtggta tagaatgcag cagtaaatgc cgaggcaatg ccccagaata acgcggcagg
     6001 agagatcgat aatgacgtcg gattaccgtg tgtcaccaat aaaaaagtcc cgatcagcga
     6061 tgtcaaaata gcgcagaaaa ccagaatgcc cgggcgggat ttacgcacca gtgagaacca
     6121 ggcgacgata atagtcggtg agaggaattg cagcaccgtt gccgtggctg cgttcgattt
     6181 ttcgatggtt agcaaaaaag tgagctgtac ggttagcgcg ccaaccacgg aaaaaatcag
     6241 taggctaatg gcatctttgt gattgttaat aatagaaaag attttatcac catgaacaaa
     6301 tgatagcgtc agcagaatca aaccggcgaa tatcaaacgc gtcatagtca aaaactgcga
     6361 cgacatctgg ctttgctcca tgatgtattg cgcgcagacc cctgaacttc cccacaacac
     6421 ggcggcaatc agaacgttca gcatcccctt tctggtggaa cccatttttc cctctgcata
     6481 ctgttttgtc gtttatgtat gcaggcatga tagcaaaaat ggcgaggatg gctcagtttg
     6541 cgccacgccg atttttctta gcccagactg gtgagctaaa tatctttaat aaacagcaag
     6601 caaatcattg caatacccaa catccctatc gccagccaga acaacgtgtg gtagctccag
     6661 atttcaacca taatttcgtc aacagagccg gcgataatct agccgaagcg tgaggtattt
     6721 gcatataacg ttgttgcgga acctattctt atttatcgga gtgccttgcc agtaatttac
     6781 tgacgaggat cctcataata gcgttaccca tctgttgcat tgagacttta caggccgtta
     6841 ttctaaatga ccaaaattaa atcagtgcat aaggggaagt attctgtagt gatgtctccc
     6901 tgagatgcag gttccactgg agaaaattat gagcagtttg tttcagaatg aaataaagga
     6961 taaatttaaa actggacttg catatgtgcg ggggccagaa aaaagcggat cttccgctca
     7021 aaatgcaggt agcgaaggtg gtaaaaaatg cgccgggaga agcagaagat tgtcccgcga
     7081 tgtccgtttg agaaacacga gtttgattaa acgcgtgagc atgatgatat tagtagctga
     7141 ccagagaaat tatatccgtc gtaagcaata aaaatcagaa gtgatgttat ggatattttt
     7201 aacctgaata actccgtaag tacggggtta ttcaggtttt tgctagcctg tgcttttcta
     7261 taccagat
//
LOCUS       DAZKFZ010000222         7247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000222 DAZKFZ010000000
VERSION     DAZKFZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.222"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            11..712
                     /gene="elfD"
                     /locus_tag="ACHOQW_004112"
     CDS             11..712
                     /gene="elfD"
                     /locus_tag="ACHOQW_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9575081.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            737..3336
                     /gene="elfC"
                     /locus_tag="ACHOQW_004113"
                     /pseudo
     CDS             737..3336
                     /gene="elfC"
                     /locus_tag="ACHOQW_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3327..4397
                     /gene="elfG"
                     /locus_tag="ACHOQW_004114"
     CDS             3327..4397
                     /gene="elfG"
                     /locus_tag="ACHOQW_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575082.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTTGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4425..4952
                     /gene="ycbU"
                     /locus_tag="ACHOQW_004115"
     CDS             4425..4952
                     /gene="ycbU"
                     /locus_tag="ACHOQW_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575083.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            4960..5475
                     /gene="ycbV"
                     /locus_tag="ACHOQW_004116"
     CDS             4960..5475
                     /gene="ycbV"
                     /locus_tag="ACHOQW_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575084.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5441..6177
                     /gene="ycbF"
                     /locus_tag="ACHOQW_004117"
                     /pseudo
     CDS             5441..6177
                     /gene="ycbF"
                     /locus_tag="ACHOQW_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6288..>7247
                     /gene="pyrD"
                     /locus_tag="ACHOQW_004118"
     CDS             6288..>7247
                     /gene="pyrD"
                     /locus_tag="ACHOQW_004118"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP9575085.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSG"
BASE COUNT         1943 a         1620 c         1858 g         1826 t
ORIGIN      
        1 gtgactattt atgaaaactt gcataacaaa gggaattgtg accgtaagtt taacggcaat
       61 attactcagc tgttcgtcca catgggcggc aggtaaaggc ggggttggac ttgcagcaac
      121 acgtcttgtt tattcggaag gtgaagagca aatttcactg ggtgtacgta acaccagtcc
      181 ggatgttcct tatctgattc agtcatgggt gatgacccca gataataaaa aatcagcgga
      241 ctttattatt acaccaccgc tatttgtgct gaatccggca aacgagaatc tgttacgcat
      301 tatgtacatt ggcgcgccgt tggcgaaaga cagagaaact cttttcttca ctagcgtacg
      361 ggcagtccct tcaacaacga agcgggaaga aggcaatacc ctgaagattg ccacacaaag
      421 cgtcatcaaa cttttctggc gaccaaaagg tttagcgtat cccttaggcg aggctccggc
      481 gaaactgcgt tgcacttcgt cagctgacat ggttacggtc agtaacccaa caccttattt
      541 cattaccctg acagacctga aaataggtgg aaagttagtt aaaaatcaaa tgatttcccc
      601 ctttgataaa taccaattct ctctgccaaa gggtgccaaa aatagcagcg taacgtatcg
      661 aaccatcaat gactacgggg cggaaacgcc gcaactcaac tgtaaatcgt aattcgtctt
      721 cagttaagag agcgagatgt atagaactca ccgacaacac agcctgttaa gctctggtgg
      781 agtgccatcg tttattggtg ggctggtggt gtttgtgtcg gcagcgttca atgcacaagc
      841 tgaaacctgg ttcgatccag cctttttcaa agatgatccc tcaatggtgg ccgatttgtc
      901 tcgtttcgaa aaaggacaaa aaataacgcc aggggtttat cgtgtcgata ttgttctgaa
      961 tcagacaatt gtagatacgc gcaacgtcaa ttttgttgag ataacgccag agaaggggat
     1021 tgccgcctgt ttgacgactg aaagcctgga tgcaatgggc gtgaatactg atgcatttcc
     1081 ggcttttaaa caactggaca aacaagcgtg tgcgccattg gcggagatta ttccggatgc
     1141 cagcgtaact tttaatgtga ataaactccg tctggaaatt tcagtaccgc aaattgccat
     1201 caaaagtaat gctcgtggtt atgtccccct gaacgttggg atgaagggat caacgcgcta
     1261 ttactgggat attcatttag cggggctaac agtattcata gcagcgcaga cagtgattct
     1321 ggcgacagtt attttctgaa tttaaacagt ggcgttaatt taggcccatg gagattgcgc
     1381 aacaattcaa catggagtcg tagtagtggc caaaccgcag aatggaagaa tctcagcagc
     1441 tatttgcaac gggcggttat tcctctgaaa ggcgaactga ccgtaggtga tgattatact
     1501 gcaggcgatt ttttcgatag tgtcagcttt cgtggtgtgc agctggcgtc agatgacaac
     1561 atgctgccag acagcctgaa aggggttgcg cctgtggtgc gtggtatcgc caaaagcaat
     1621 gcccagataa cgattaagca aaatggttac accatttacc aaacttatgt atcgcctggt
     1681 gcttttgaaa ttagtgatat ctattccacg tcgtcgagcg gtgatttgtt agttgaaatc
     1741 aaagaagcgg acggcagcgt caatagctac agcgtaccct tttccagcgt gccattactc
     1801 cagcgtcagg ggcgaatcaa atacgcggtg acactggcga aatacagaac caatagtaat
     1861 gaacagcagg agagcaaatt tgcccaggcc acgttgcagt ggggcggacc gtggggaacg
     1921 acctggtatg gtggtggaca atatgctgaa tattaccgtg ccgccatgtt tggtctggga
     1981 tttaacctcg gcgatttcgg agcaatttcg ttcgatgtga cccaggcgaa gagtacgctg
     2041 gcagaccaaa gcgaacataa aggtcagtca tatcgttttc tgtatgccaa aacgctcaac
     2101 caattgggta ctaactttca attgatgggc tatcgctatt cgacgtcggg tttctacacc
     2161 ctttccgaca ccatgtataa acatatggat ggctacgaat ttaatgacgg tgatgatgaa
     2221 gatacgccga tgtggtcgcg ttattacaat ttgttttaca ccaaacgtgg caaactgcag
     2281 gtcaatatct cccagcaatt aagcgagtac ggttcgtttt atttaagtgg tagccagcaa
     2341 acttactggc ataccgatca acaggatcgg ctattacagt ttggctataa cacgcaaatt
     2401 aaagatctct cgctggggat ctcctggaac tacagtaagt cccgtggcca acctgatgct
     2461 gatcaggtgt ttgcacttaa tttttccctg ccactcaatc tgttgctctc cagaagtaat
     2521 gatagctata ccagcaaaaa aaattacgcc tggatgacct ctaacaccag tatcgataac
     2581 gaagggcaca ctacacaaaa cctgggttta acggagacac tactcgatga cggtaacctg
     2641 agctacagcg tgcaacaggg atataacagc gaggggaaaa cggctaatgg cagcgccagc
     2701 atggactaca aaggggtgtt tgcagatgcc cgagtgggct acaactacag cgataacggc
     2761 agtcaacaac aactgaacta cgctctttca ggcagtttag ttgcccattc acagggcatt
     2821 accctggggc aatcactggg ggaaactaac gttctgattg cagcaccagg cgcagagaat
     2881 actcgtgtgg cgaacagcac cgggctgaaa actgactggc gcggatatac cgttgttcct
     2941 tatgccactt cttatcgtga aaatcgaatc gcacttgatg cggcgtcgtt aaaacgtaac
     3001 gtggatcttg aaaatgcagt agtcaacgtg gttcccacca aaggggcgtt ggttctggcg
     3061 gagttcaatg cccatgcggg agcaagggta ttaatgaaaa catcaaagca gggtatatcg
     3121 ctgcgttttg gcgcgatagc gacgctggac ggcgtacaga ctaatagcgg cataattgat
     3181 gatgatggct cgctctatat ggcgggttta ccggcgaagg gaacaataac cgtgcgctgg
     3241 ggcgaagctc ccgatcaaat ttgtcatatc agttacgagc ttaccgaaca acaaattaac
     3301 tctgcgatta cgcgaatgga tgccatatgc agataatctt tggagaaaaa tgcgtgtcat
     3361 tactacgact attttttgcc gccgtcttaa tgctatggtg cgctcaaacc gctgcttata
     3421 gcgggcagtg tcataccact caggggaatc cgtatattgg cgtcaatttt ggcgttaaaa
     3481 ccctggagga agaagaaaat acgactgggg tagtaaaaga caaattttat cagtggaacg
     3541 aatcgaatga ttattatgtt tcctgtgatt gcgataaaga caatgtcaga agtggccgat
     3601 gggcattcgc cgcggattca ccgttagtct atttaggcga caactggtac aaaattaatg
     3661 actatcttgc cgccaaagtt ttattgcagg ttaaaggcag ttctcctaca gcggttcctt
     3721 tcgaaaacgt ggggactggg gcagataccc ggtggcatat ttgtgacccc ggcggtcaac
     3781 gtttaggcgg ccagggagct agcggtaata gcggtagctt ttccctgaaa atattgcagc
     3841 cgttcgttgg ttcggtcgtc attcctccta tggcgctggc gcgattattt gaatgctaca
     3901 acatacccgc aggtgattcc tgcacgacta caggcacacc ggttttagtg tattacctgt
     3961 ctggtactat caattcactt ggctcatgtt ccgtcaatgc cggagaaaca atcgaggtcg
     4021 atctgggcga cgtatttgcg gctaactttc gtgttgtagg gcataagcct cttggggcca
     4081 gaacggcaga acttgcaatt ccagtcaggt gtaacacggg aaacgcgggg ttagttaacg
     4141 tcaacctgag tctgacggca accacagacc ccagctatcc ccaggcgatt aagacgtcac
     4201 gtcctggcgt gggcgtggtg gtgaccgata gccagaacaa cattatttcc cctgctggtg
     4261 gaacattacc gctctctatt cctgatgatg cagacagtat cgcgcgaatg aatgtctatc
     4321 cagtcagcac gacaggtgta ccaccagaaa ccgggcgatt tgaagccacg gcaacggtga
     4381 gaataaattt tgattaacgc gtgaacgtat gaagaaaaaa aacgatattt cagtgcgtta
     4441 tcttgttctt tagcattctt aacatccatg tcgggatggc tgggcctgaa caagttagta
     4501 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4561 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4621 ctgctgctga tttgaatatc gatatcaccg gttgtgccgc tggtattact ggcgcggacg
     4681 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4741 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4801 aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4861 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4921 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4981 attctgttct tactgggatt aacggggggg tgtgagctat ttgcccatga tggcacggtc
     5041 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     5101 aatgtgcagt taggcgatgt ctcactgaca cgtttttctc atgggaatta tggcccggag
     5161 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgacgtcacc
     5221 ttttcaggaa cccccgacgg agtgcagagc gaaatgttgt ccattgaaag tggtacggat
     5281 gcagccagcg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5341 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5401 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5461 cttcattatg actaatacgt ggaacagatt ggcgctcttg attttcgccg ttttatcgtt
     5521 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5581 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cctggcttat
     5641 taacagtaaa atcaaccgcc caacgcgttg ggccgggggt gaagcgtcga cagtgccagc
     5701 accattactg gccgctccgc cacttattct cctgaagccc ggtacgacag gcacgttgcg
     5761 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5821 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5881 ggtatttaaa ctgttctggc gacccgaagg ttgccgggcg acccgctgga agcttatcaa
     5941 caattacgct ggacacggaa gtcgcagggt gtacaactca ctaacccaac gccttattac
     6001 attaacctga ttcaggtgag cgtaaatggt aaagcgttaa gtaatgtggg agttgtgccg
     6061 cctaaaagcc agcgtcagac aagctggtgt caggcaatag caccgtgtca tgttgcctgg
     6121 cgggcgataa atgattacgg cgggttgagt gcaaagaagg agcaaaatct gccctgaaac
     6181 aggttcggaa aacgtttgcg ttttttttgc cgcaggtcaa ttcccttttg gtccgaactc
     6241 gcacataata cgcccccggt ttgcacaccg ggaatccagg agagttcatg tactacccct
     6301 tcgttcgtaa agcccttttc cagctcgatc cagagcgcgc tcatgagttt acttttcagc
     6361 aattacgccg cattaccgga acaccgtttg aagcactggt gcggcagaaa gtgcctgcaa
     6421 aacctgttaa ctgcatgggc ctgacgttta aaaatccgct tggtctggca gccggtcttg
     6481 acaaagacgg ggagtgcatt gatgcgttag gcgcgatggg atttggatcg atagagatcg
     6541 gtaccgtcac gccacgtcca cagccaggta atgacaagcc gcgtctcttt cgtctggtag
     6601 atgccgaagg tttgatcaac cgtatgggct ttaataatct tggcgttgat aacctcgtag
     6661 agaacgtaaa aaaagcccat tatgacggcg tcctgggtat taacatcggc aaaaataaag
     6721 atacgccagt agagcagggc aaagatgact atctgatttg tatggaaaaa atctatgcct
     6781 atgcgggata tatcgccatc aatatttcat cgccaaatac cccaggatta cgcacactgc
     6841 aatatggtga agcgctggat gatctcttaa ctgcgattaa aaataaacaa aatgatttgc
     6901 aagcgatgca ccataaatat gtgccgatcg cagtgaagat cgcgccggat ctttctgaag
     6961 aagaattgat ccaggttgcc gatagtttag ttcgccataa tattgatggc gttattgcaa
     7021 ccaataccac actcgatcgt tctctggttc agggaatgaa aaattgcgat caaaccggtg
     7081 gcttaagtgg tcgtccgctt cagttaaaaa gcaccgaaat tattcgccgc ttgtcactgg
     7141 aattaaacgg tcgcttaccg atcatcggtg ttggcggcat cgactcggtt atcgctgcgc
     7201 gtgaaaagat tgctgcgggt gcctcactgg tgcaaattta ttctggt
//
LOCUS       DAZKFZ010000223         7227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000223 DAZKFZ010000000
VERSION     DAZKFZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(245..838)
                     /gene="tehB"
                     /locus_tag="ACHOQW_004119"
     CDS             complement(245..838)
                     /gene="tehB"
                     /locus_tag="ACHOQW_004119"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP9575086.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(835..1827)
                     /gene="tehA"
                     /locus_tag="ACHOQW_004120"
     CDS             complement(835..1827)
                     /gene="tehA"
                     /locus_tag="ACHOQW_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP9575087.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            1951..2931
                     /gene="ydcK"
                     /locus_tag="ACHOQW_004121"
     CDS             1951..2931
                     /gene="ydcK"
                     /locus_tag="ACHOQW_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP9575088.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2926..3462)
                     /gene="rimL"
                     /locus_tag="ACHOQW_004122"
                     /pseudo
     CDS             complement(2926..3462)
                     /gene="rimL"
                     /locus_tag="ACHOQW_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(3525..3749)
                     /locus_tag="ACHOQW_004123"
     CDS             complement(3525..3749)
                     /locus_tag="ACHOQW_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP9575089.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(3889..5544)
                     /gene="opgD"
                     /locus_tag="ACHOQW_004124"
                     /gene_synonym="mdoD"
     CDS             complement(3889..5544)
                     /gene="opgD"
                     /locus_tag="ACHOQW_004124"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP9575090.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5769..7112)
                     /locus_tag="ACHOQW_004125"
     CDS             complement(5769..7112)
                     /locus_tag="ACHOQW_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9575091.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         1901 a         1818 c         1703 g         1805 t
ORIGIN      
        1 tgttgtggca aaaaaagtgt tggtgaaaat aaattttttt agcccctttt cagacgacat
       61 agtcaatgcc gcaaaagggg ctaagtcaat catcagtagt taaatttcag taagttaagc
      121 tgctaaaaaa cttcgactac tgtgattaat ttcacatccc ttcgataaaa aatatgctta
      181 ccgaaaagaa ggacagtaaa ccatctcagc gtggggaatt ttcacgcgct ttgttcttac
      241 cgggtcattt tttacgtgcc agcatcgtgg cgaaacgcag tttaatacga ttaccgttgg
      301 cgtcggtgcg gtgcagctcg ccgacgtctt cattgtattt caccatctcc cagccttcgt
      361 aatatcgacg taattctccc tctttgaagg caaacgggaa gccgacggta catggataat
      421 cagcggtatc catcgccgcc acaatcaggt tgtaaccgcc aggtttagtg caacgttgca
      481 tattggcaat cagccctggg atggttttag cctcaaggaa catcagcacc acagtcgaaa
      541 gaataaaatc gtactctcca tcaaatgtga ggttattcag atcaacgacc cgggtgtgta
      601 aattatccag attttcaatg gatttaatgc gctcgacgtt ggcgatactc atggcatttt
      661 tatcccatgc gtcaacatca taaccattgg ctgccaggta aagactgtta cgaccattgc
      721 cacagcccag atccagcgtt ttacccggtt taaccacttt caccgcttcc agtacttcag
      781 agtgtgtgcg ggttaattca tatttatcag taaaataatt ttcgtcacga atgatcattc
      841 tttgtcctct gctttcatta aaacggcgcg ctcggttctg actaacaatt ttccctgcat
      901 cagaagcgcc aaagtacgga tgagcagtat tgcaataata aaattggtaa agataaacag
      961 cggcaccgcc agcgtatgga aaaatccatt atcgctgcca ctccccagat gcaaaccggt
     1021 ggttgccagt gcagatacgc cgaacgagaa actccagaat gaagcattaa atggctggga
     1081 gagataccat ggcatcagac gtagcataaa cagcagttgc agcagtccat aaccgaaaag
     1141 cattttcgcc agcgtgtcac cctcgccgcc gttgacgctc agccaggcac tacaggccac
     1201 cagcgcagga gcgagctgaa tgccgagtga tgtccgcagt gccgtgggta attctcccga
     1261 actacgcaga cgctgcaaga tcaccggttc aaggcttagc catgagaaaa cgcctgcgcc
     1321 taaaaacacc agaccggcgt cggtgtagcc caacgcacca caggccattg cgctgataaa
     1381 gttgttggca actgtcggca gatacagtcc aggcgtagta gcttcttcag ggtgagatcc
     1441 gcgccataat cccgcagttt gccaggcggc ataagccaac tgaaccacga caccaaaact
     1501 gaacaggcac accgccagtg ggcgaaacca cggaacaaaa ccaatcgcca ccagcatcgt
     1561 cgttgcagga aacaaactca caaaactgct cagcactgga tggcgaactt ccgccagcac
     1621 gctatgcgga aagcgtatga gtcgggtaat aaatgcgcta gtcaataatc cccagatgat
     1681 catcgccaga atcaccagcc catcccctaa ccagtggctg acctgccaaa cctggctggc
     1741 atagcgccag gcaaatccca tccctatcgt ccccaacaca ataccaaagt agcctgccgg
     1801 caaattgagc actttatcgc tctgcatctt cttactcatt ttgtttaatt tataaagtat
     1861 atttgaaatg cattttatgg aattttctcg agcatagcca gagccgcaga atttgctacg
     1921 gttatagtaa gtcacctgtg ggaaaacgct atgcgcaaat atcgtctaag tgaagaacag
     1981 cgagccttta gttatcagga agatggcact aaaaaaaatg tgttattacg gcagattatc
     2041 gccattagcg attttaacga tgtgatagca ggaaccgctg gcggctggat cgatcgcgaa
     2101 acggtgttag cacaggaagg aaattgctgg atttacgacc agaatgctat tgcgttcggc
     2161 ggaacggtga tttccggtaa tacgcgcatc accggcacca gcgtactgtg gggagaggtt
     2221 tacgcaacgg ataatgtctg gatcgataat agtgaaatca accagggtgc atatataagt
     2281 gacagtgtca ccattcatga ctcactggtt tgcgggcaat gtagaatatt tggtcacgcc
     2341 ttaattgatc aacattctat gatcgtcgcc gcacaaggct taacgcccga ccatcaactc
     2401 ctgctccaaa tttatgatcg cgcaagggtt agcgcctcgc gtattgtcca ccaggcacaa
     2461 atttatggcg atgccgtcat ccggtatgct tttattgaac atcgcgccga agtttttgat
     2521 tttgccagta ttgaaggtaa tgaagagaac aacgtctggc tatgcgattg cgctaaagtc
     2581 tacggtcacg cgcaggtgaa agctggcata gaagaagatg ccattccaac gattcattac
     2641 agttcgcagg tggcggaata tgccattgtg gaaggtaatt gtgtgttaaa acatcatgtc
     2701 ttgattggcg gaaatgccgt ggtacgtggc ggaccgattc tgctcgacga gcacgttgta
     2761 atacaaggtg agagccgtat tacaggtgcg gtgataattg aaaaccatgt ggagttaacc
     2821 gaccacgccg tcgttgaagc ctttgatggc gacacggttc atgtccgtgg tccaaaagtc
     2881 attaacggcg aggagcgcat tacgcgaacg ccgctggcag ggttattatg aatcgataat
     2941 acgcgcgtat aagttcacat catcataggc atcattcagg aactcagcct gtttcaggca
     3001 accttcaagg ataaaaccat tgcgcaaagc gacctggttg ctttgcggat tgtccacccg
     3061 acatttgatc acgaagcgtc taagttcacc agactgagcg taatgatgaa tcagtgcctg
     3121 cagcgcctga gaaatgatcc cctgcccctg atgagattcg tccagtcagt agccgatttc
     3181 agcggtttta ttcagtggtt caatacgatt aaacgagata acgccgataa gttcatcttc
     3241 tttgaaaatc atgaacattt tggcatagcc gcgttgatgc aacatcacat taccctgcac
     3301 cgtttttcgc gtgtcctctt caatctgaac gaactgcggc cagtttagcg actgctgtaa
     3361 ccaggtttta tttttacaga ttaactgata aagaggagtg acgtgatttt ctgcaacagc
     3421 atgtaattca agtgattcgc ttacttttat cgtttcagtc atcttatctc cagtcaatcc
     3481 atgcagttgt taaaaaagcc gcctggctgg cggcttagga aagtttacac ctctttgacg
     3541 ctctcatgct gcaggatttt gagcatctca tcttttaact gtagcttttg ttttttcatg
     3601 cggaccactt ccgcattgta ccctcgatcg tcggaacctt cctttctggc aatttcatga
     3661 tcaagtttat tgtgtttatc gaacaaggac ataaagcgag gattttcgtt tttcagacgg
     3721 gatattaaat ctcgatattc tggaaacata cgcactccct gttagtggct gacaaggtgt
     3781 atctgaaata cacaccatca gattactcat ttaacttagg gaaaaatgcg agcgggatcg
     3841 tgtttaacca gacggcacct cccgaagaag gtgccgaaga aaaaacgatt aactcatcac
     3901 gcggtcgtca acatactgac gtttatccgg cgctggcggg aaatactgat acagccatgt
     3961 ttcactgata gcgtcgccct gacaacgcag atacatccgc atatcgaccg gatcagtgga
     4021 gtccgaagtc ggataccagt caaactgaat acgataacca tcaatgggtt caatatagag
     4081 aatttcgatt tgcttcgctt ccccactgga aagcgtaatc accggctcaa tgccttttgg
     4141 tgcggcagct ttcagatcac caccaacgaa atcgacggca aaacgacgcg cccatttttc
     4201 gggatagtgt tcacctggtg cccaaccttc cgggaaaccg cccatgccgg tacgcgtcgc
     4261 cataacgcgc gctaatgggc aatgaacagg cggttgcgca ctccagtaca gacgatactg
     4321 gaatgcaaac tcatcacccg cctttacagc tttttctggc tgccagaagc agacaatgtt
     4381 atccagcgtt tcgcccgttg ttgggatttc catcaggccg atggtgccct taccccactt
     4441 gttacgcggt tccacccaca gacttgggcg tttgttatac cagcccataa tgtcctgata
     4501 atgggagaaa tcacgatcca gttgcaataa accaaaccct ttcgggttgt tgtcggtgta
     4561 agcattgaac tgcaattttt gcggattatt cagcggacgg caaatccact cgccgttgcc
     4621 ccgccacatg gacagacgat cagagtcatg aatttgcgga tgaatggtgt cgcacatccg
     4681 acgttcatta gtaccgcagc tgaacatact ggtcatcggc gcaatgccca gctgtttaat
     4741 gtctttgcgc gcatacaggt gattttccac atccataatc acctgacttt tctcacaatg
     4801 gatagtgaac ttataggcac cagtaatgct ggcgctatcg agcaacgcat aaacggtaaa
     4861 ggtagttgcc cccggtttta ccgtatcaaa ccagaaggcg gtaaagtcgg ggaactcttc
     4921 tttactgtcg gtgtaagtgt caatcgccag gccgcgggcc gacaaaccgt attgatatgt
     4981 gtcatcaacg gcgcggaaat aactcgctcc gaggaatgat actacatcac ggcgcgccag
     5041 ttcgggggct ttaaacacgc gaaaaccggc aaagccgaga tcgctttgcc cttctaattg
     5101 tttggtatca acacctgcat cgttgtattt gaacaactcc gggcgaaagt gaatttcacg
     5161 cgccagatgt gttgctggat ctacagaaaa catacgaacg cggcgacgga atcccattcc
     5221 catatggaag aactgagcgt ccagttgacg gttctcaacg ttatgccaga gcgatttttc
     5281 ggcgtcgtat tgaatactgt tataagcctg cggcgtcatt gtcgccagcg tgtcaggtaa
     5341 cggacgaggc gcaccacgcc acgctgtttg cgctaagtcg tgcgccattg actgtagaat
     5401 ggagaagtca aaacgctggg tttgcccgtc ggcaatatca gaatctgccg cgaatgccgc
     5461 ctgagaaaaa agagaagcaa tgccgctggt accgcacacg gcggccatag ccattgaacc
     5521 tttaataaat cgtctacgat ccatacctga aagtgagtcc ttctggtgtt gtgaatggtc
     5581 ccgcgctggt cgttttcagc ggcaagaaag tgcttattta tgaccgctca ctctagacaa
     5641 tatttattaa taatccaatt gttgaactga gagaatatgc ataaatcggc aattttttta
     5701 tgtcgatggg atgagcctga ttcgtttgta aaatcaggca aatttttgca aaggcggatg
     5761 gtcagaattt aaagcaaacc acagcgacgt ttactgcgtt cttccgcttc ctgataaagc
     5821 tggaactcat ccaaaaccgg acaacccaac gcctgctcaa atgcttcgcg actggcatta
     5881 ccgtggctgc gtgcctgcgt ttcattaccc agatttgact ggaagatccc cgccgcgcta
     5941 acgggcaaga aatcttcata ggtaatgggt tgcgccgcga cccaaccacg ttcaattaag
     6001 ggctgtggat cgtctccggg atgaatcgcc tgacgatgcg cctcacctga aggcgtcaga
     6061 cggtaccgga accatgccag cccttgctga cgcattaaaa actcactgtc aggaaaagtg
     6121 cggaaggttt cctgtaaatg catttggtga gtgagattat cctgcccggt tccagcgtta
     6181 cgcagaagat catcatacag ttgtcgacct ttcggcgtta atgccacgcc acgctgctca
     6241 atttcaccaa agcgcgcagt atgcgtgccc tgtttctgcc ccgcaaacaa caccgtctct
     6301 tccagtgctt taaagctggt ctggcgtagt aaaatcggta cctcgcggcg cggcggccct
     6361 tcgatcagaa tttttggttc aattccgcat tcaggcatca tcgactgcac ccggtcaata
     6421 tccagcgtac gtggcgtcag atggttgata tggcatccag gaaaacagac cacatcagca
     6481 atcaaccgat gttcgttgtg caaggcgcga taggtttctt catctaccgt tgctgactgg
     6541 tgccaacgga acgtttccag ggcctcctgc acaaactcct gtgcctgtgt ttcgttaaaa
     6601 ccgccccgct ggtcatactc ctctaacagt tgtcgacaac gtggggtgaa gatatcgcgc
     6661 tgacgcagaa tctccgccgc tttctggcgc aaaatttcgt tctcgataag ctcaaggcgg
     6721 agtaaggagg taaaaacgcg gaagggatta cgcgccagag aagcatcatc aatgggccga
     6781 aatgctgtcg aatgtaccgg cacccctgcc tgcgagagat cgtaatagct caccgggtac
     6841 atccccataa tggcaaacat ccgccgaaga gtagcaagct cttgtgcagt cccaacgcga
     6901 atcgccccat gacgttcaac attcagtcgc gccagctcgt ctgcatttac cattttttcg
     6961 tgcagttgag gattgttttc cagcacagcc agattcacat cagctaccag ttccagcagc
     7021 gtgccatatt gcggaacttc ttgctggtac atggctgaca ttgcctgcga aaactgttcc
     7081 cgaatctcat ccgccgtgat gctgttcgcc atagtgtctg cctccagtgg tcagtaatct
     7141 ggagtgtagg taaccgcatt cactctggcg ggaagaattt acaaactgtg atctcgccgc
     7201 gaaaacatca atattatcca ttttgct
//
LOCUS       DAZKFZ010000224         7216 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000224 DAZKFZ010000000
VERSION     DAZKFZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1661)
                     /locus_tag="ACHOQW_004126"
     CDS             complement(<1..1661)
                     /locus_tag="ACHOQW_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9575092.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
     gene            complement(1636..2181)
                     /gene="nohD"
                     /locus_tag="ACHOQW_004127"
     CDS             complement(1636..2181)
                     /gene="nohD"
                     /locus_tag="ACHOQW_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP9575093.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2570..2764
                     /gene="ylcI"
                     /locus_tag="ACHOQW_004128"
     CDS             2570..2764
                     /gene="ylcI"
                     /locus_tag="ACHOQW_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9575094.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            complement(2929..3135)
                     /gene="ybcW"
                     /locus_tag="ACHOQW_004129"
     CDS             complement(2929..3135)
                     /gene="ybcW"
                     /locus_tag="ACHOQW_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFP9575095.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            3421..3831
                     /gene="ybcV"
                     /locus_tag="ACHOQW_004130"
     CDS             3421..3831
                     /gene="ybcV"
                     /locus_tag="ACHOQW_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP9575096.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            4123..4416
                     /gene="bor"
                     /locus_tag="ACHOQW_004131"
     CDS             4123..4416
                     /gene="bor"
                     /locus_tag="ACHOQW_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFP9575097.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(4507..4689)
                     /gene="rzoD"
                     /locus_tag="ACHOQW_004132"
     CDS             complement(4507..4689)
                     /gene="rzoD"
                     /locus_tag="ACHOQW_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP9575098.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(4906..5382)
                     /locus_tag="ACHOQW_004133"
     CDS             complement(4906..5382)
                     /locus_tag="ACHOQW_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9575099.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(5369..5674)
                     /locus_tag="ACHOQW_004134"
     CDS             complement(5369..5674)
                     /locus_tag="ACHOQW_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9575100.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(5996..6685)
                     /locus_tag="ACHOQW_004135"
     CDS             complement(5996..6685)
                     /locus_tag="ACHOQW_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9575101.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(6682..6822)
                     /locus_tag="ACHOQW_004136"
     CDS             complement(6682..6822)
                     /locus_tag="ACHOQW_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9575102.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(6819..7181)
                     /gene="rusA"
                     /locus_tag="ACHOQW_004137"
     CDS             complement(6819..7181)
                     /gene="rusA"
                     /locus_tag="ACHOQW_004137"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9575103.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
BASE COUNT         1884 a         1865 c         1599 g         1868 t
ORIGIN      
        1 agatcaaaaa tatccgggtt attcgggaag tgaacggcac cgggaagcgg ttcatcccct
       61 tccggcgtca gtgtgaagcg gttataaatc tgctctttcg cggtatccgt accgatttcg
      121 gtaaggtaaa ccccgttttt gtttcgctta cgtggcatgc tggccaccgg ctttccgtag
      181 acggatgccc ctttaatggg gatcacccgg aacagcccat gctttttcga gcgttcatac
      241 acaatagtcg ggtcaatccc gccagtatcc cagcagatac gggataccga catttctgca
      301 ccattccggc gggtataggt tttattgatg gcctcatcca cacgcagcag cgtctgttca
      361 tcgtcgtggc ggcccataat aatctgccga tcaatcagcc agctttcctc acccggcccc
      421 catccccata cgcgcatttc gtagcgatcc agctgggagt cgataccggc ggtcaggtaa
      481 gccacacggt caggaacgga cgctgaataa tgctctttcc gctctgccat cacttcagca
      541 tccggacgtt cgccgatctt cgcttcccat gtctcaccga gcgtggtgtt cacgaaggtt
      601 ttacgttttc ccgtatcccc tttcgtcttc atccagtctt tgacaatctg aacccaggtg
      661 gtgaacgggc tgtacgccgt ccagatgtga aaggtcacac tgtcaggcgg ttcaatctct
      721 tcaccggatg acgaaaacca gagaatgcca tcacgggtcc agatcccggt cttttcgcag
      781 atataacggg catcagtaaa gtccagctcc tgctgacgga tgacgcaggc attatgctcg
      841 cagagataaa acacgctgga gggatcatcc ggcgtccatt tgaggccaaa cggcgtctct
      901 ttatcgccaa atttaaggta ctgctcctcc ccgcagtgcg ggcaggcaac atgaaaacgc
      961 ataaaatgcg gggattcact ggctgcacgc tcaatctggc aggtgcctct cactttgggc
     1021 gtggagccac ggatggactt tggccagacc gagccttcaa tacgcttatc gcccaggaac
     1081 gtcggagagc cttcctgttc aatatcctca tcaaaggcag caagttcatc ataacccgcc
     1141 acatccactg acttttcacg gtagtttttt gccgctttac cgcccaggca ccagaagcca
     1201 cgcccattgg tgaaacgctt catggtgagc gtgttatccc ggtgcttttt gccataccac
     1261 ggggccagcg ccagcagcga cggaatatca cgaatagtcg gctcaacgtg ggttttcata
     1321 aagttctcgg catcaccatc cgtcggcaac cagataaggg tgttgcgctg cttatgctct
     1381 atgaagtagg cataaacacc cagcagcatt ttggaataac caacacgggc agacttcacc
     1441 acattcacct cgcggatgta gtcactgccc atcgcattca tgatggcccg ctgaaagggc
     1501 agtgtttccc agcgcccttc ctggtatgcg gattcttttg ggagatagta actggcatcc
     1561 gcccattcaa cggcggtctg tggctccggc ctgaacagtg agcgaagccc ggcgcggaca
     1621 aaatgccgca gcctgttaac ctgactgttc gatatattca ctcagcaacc ccggtatcag
     1681 ttcatccagc gcggctgctt tgttcatggc tttgatgata tcccgtttca ggaaatcaac
     1741 atgtcggttt tccagttccg gaaaacgccg ctgcaccgac agggggatcc cgtcgagaat
     1801 actggcaatt tcacctgcga tccgcgacag cacgaaagta cagaatgcgg tttccaccac
     1861 ttcagctgag tctctggcat tcttcagttc ctgtgcgtcg gcctgcgcac gcgtaagtcg
     1921 atggcgttcg tactcaatag tccctggctg gagatctgtc tcgctgtcct gcctcagttc
     1981 ttcaacttcc cggcgcagct tttcgttctc aatttcagca tccctttcgg cataccattt
     2041 tatggcggcg gcagagtcat aaagtacctc attacccttg ccaccacccc gcagaacggg
     2101 cattccctgc tcctgccagt tctgaatggt acggatactc gcgccgaaaa tgtcagccag
     2161 ctgctttttg ttgacttcca ttgttcattc cacggacaaa aacagagaaa ggaaacgaca
     2221 gaggccaaaa agcccgtttt cagcacctgt cgtttccttt cttttcaggg ggtgttttaa
     2281 ataaaaacat taggttacgg cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat
     2341 aaatagtgaa aaaccgcgag gtcgccgccc cgtaacctgt cggatcgccg gaaaggaccc
     2401 gcaaaatgat aataattatc atctacatgt cacaacgtgc atctacgcca tcaaaccacg
     2461 tcaaataatc aattatgacg caggtatcgt attaattgat ccgcatcaac ttaacgtaaa
     2521 aacaacttca gacaatacaa atcagcgaca ctgaatacgg ggcaacctca tgtcaacgaa
     2581 gaacagaacc cgcagaacaa caacccgcaa catccgcttt cctaaccaaa tgattgaaca
     2641 aattaacatc gctcttgatc aaaaagggtc cggaaatttc tcagcctggg tcattgaagc
     2701 ctgccgtcgg aggctaacgt cagaaaagag agcatataca tcaattaaaa gtgatgaaga
     2761 atgaacatcc cgcgttcttc cctccgaaca ggacgatatt gtaaattcac ttaattacga
     2821 gggcattgca gtaattgagt tgcagtttta ccactttcct gacagtgaca gactgcgtgt
     2881 tggctctgtc acaggttaag tagtttgaat gattagcagt tatggtgatc agtcaaccac
     2941 cagggaataa tccttcatat tattatcgtg cttcaccaac gctgcctcaa ttgccctgaa
     3001 tgcttccaga gacaccttat gttctataca tgcaattaca acatcagggt aactcataga
     3061 aatggtgcta ttaagcatat tttttacacg aatcagatcc acggagggat catcagcaga
     3121 ttgttcttta ttcattttgt cgctccatgc gcttgctctt catctagcgg ttaaaatatt
     3181 acttcaaatc tttctgtatg aagatttgag cacgttggcc ttacatacat ctgtcggttg
     3241 tatttccctc cagaatgcca gcaggaccgc actttgttac gcaaccaata ctattaattg
     3301 aaaacattcc taatatttga cataaatcat caacaaaaca caaagaggtc agaccagatt
     3361 gaaacgataa aaacgataat gcaaactacg cgccctcgta tcacatggaa ggttttacca
     3421 atggctcagg ttgccatttt taaagaaata ttcgatcaag tgcgaaaaga tttaaactgt
     3481 gaattgtttt attctgaact aaaacgtcac aatgtctcac attatattta ctatctagcc
     3541 acagataata ttcacattgt gttagaaaac gataacactg tgttaataaa aggacttaaa
     3601 aaggttgtga atgttaaatt ctcaagaaat acgcatctta tagaaacgtc ctatgatagg
     3661 ttgaaatcaa gagaaatcac atttcagcaa tacagggaaa atcttgctaa agcaggagtt
     3721 ttccgatggg ttacaaatat ccacgaacat aaaagatatt actatgcctt tgataattca
     3781 ttactattta ctgagagcat tcagaacact acacaaatct ttccacgcta aatcatcacg
     3841 tccggtttct tccgtgtcag caccggggcg ttggcataat gcaatacgtg tacgcgctaa
     3901 accctgtgtg catcgttatt aattattccc ggacactccc gcagaggagt ttccctgtca
     3961 gggctgcgga catagttaat ccgggaatac agtgacgatc cttcgcatct gacatacatt
     4021 aataaatatt aacaatatga gatttcaact cattgtttag ggtttgttta attttctaca
     4081 catacgattc tgcgaacttt aaaaagcatc gggaataaca ccatgaaaaa aatgctactc
     4141 gctactgcgc tggccctgct tattacagga tgtgcccaac agacgtttac tgttcaaaac
     4201 aaaccggcag cagtaacacc aaaggaaacc atcacccacc atttcttcgt ctctggaatt
     4261 gggcagaaga aaactgtcga tgcagctaaa atttgtggcg gcgcagaaaa tgttgttaaa
     4321 acagaaaccc agcaaacatt cgtaaatgga ttgctcggtt ttattacttt aggcatttat
     4381 actccgctgg aagcgcgtgt gtattgctca caataattgc atgagctgcc catcgatatg
     4441 ggcagctcta tctgcactgc tcattaatat acttctgggt tccttccagt tgtttttgca
     4501 tagtgatcag cctctctctg agggtgaaat aatcccgttc agcggtgtct gccagtcggg
     4561 gggaggctgc attatccacg ccggaggcgg tggtggcttc acgcactgac tgacagactg
     4621 ctttgatgtg caaccgacga cgaccagcgg caacatcatc atgcagagca tcattttcag
     4681 ctttcgcatc agctaactcc ttcgtgtatt ttgcatcgag cgcagcaaca tcacgctgac
     4741 gcatctgcat gtcagtaatt gccacgttcg ccagcttcag ttctctggca tttttgtcgc
     4801 gctgggcttt gtaggtaatg gcgttatcac ggtaatgatt aacagcccat gacaggcaga
     4861 cgatgatgca gatgagcaga gcggagataa tcgcggttat tctgctcata cctcactctc
     4921 tctgaccgtt ccgccagctt ctttgaattt tgcaatcagg ctgtcagcct tatgctcgaa
     4981 ctgaccataa ccagcgcccg gcagtgaagc ccagatatta ctgcaacggt cgattgcctg
     5041 acggatatca ccgcgatcaa tcataggtaa agcaccacgc tctttaatct gctgcaatgc
     5101 cactgcgtcc tggcttttgg gggagaagtc tttcaaacca agctgtttac ggtaagcatc
     5161 ccaccagcgt gaaagaagct gataacgtcc ggcggctgtt gatttgagtt tggggtgtag
     5221 cgtgacaagt ttgcgagggt gatcggagta atcagtaaac agttcgccgc caacaataac
     5281 atcataaccg tggttacgtg tcggttgtcg cccgttatcc gttccttctg accacgccaa
     5341 catatcgagg aaggctttac gctgaggatt aagattttgc atttttcacc cctgtcagtc
     5401 gttcccagaa gtacgtcagt gcaaccgaac ccatcgcacc actaatcccc gctgtcgcga
     5461 gaatcatgta aatactgaat ccactttcga tgctgatcag gccaccaata acaccggtga
     5521 atcctgatac cactatttga gccagagcat ttatccaact ccacgttgct ttactctgct
     5581 tcacatctat caggtagcgg accagaccgc cccaacctgc gatgatcagc aaaacgagcc
     5641 agaacgctcc ggcaaggctc tctttttcgt gcatatgaat agccaatgtt tcgccgccga
     5701 caaaaggccg ggacgttaaa tgtcagaaat caggctcacg gggtaattta acgacaaagc
     5761 acggagttga tgctccccac aagcctggaa taaaaaagcc agcatgtagc tggcaacaga
     5821 gggctaagca atatcaactc aacagctgaa gaccccctgg ctggggtagg ttggaaggct
     5881 actcaccgtt cagaaacaga aaagcccaag gctttaaacc tcgggcttga atttggatta
     5941 ctgccagtgc gtacaacatt ggcaaaatat cagatttaca taaaatatat gatttttaat
     6001 ccagttttgc aatatcttgc tgtgaaaatg tggtcttttg ttttgaacgt gttttcgtta
     6061 aaagcaataa agcttggcta tcaagctgta gaaaaatgtg tttcattgca acccagcgtt
     6121 cagtaaatgt ctcagaccag ttttttgatg tcactcccac cagtgatgcc agctcctggt
     6181 attcataggt cttacgccct gccagctcgt tcttcacatc ctgtgccgcc agccagatca
     6241 acttcttcaa acgttccagt gtcttacctg caattttcct ggtacccaac agagtcttaa
     6301 actcgctcca tgcccactgc gttatggtga cctgatgttc ccagcgaaca ctttcgctgt
     6361 aactccacag caaccacgct ttctgatgtt cttcgagaga cagaaccgcg cggcgccatg
     6421 acgaggttga gaactcaacc tggctgacca gtgcaatgga tgaacctttt gcgtacgact
     6481 gcttaccgga aatcggcgga ttatccagcg taatcatcct gccagttacc tcatccagaa
     6541 tgcgcggctt ctttcgtttg tatgtaccag tatcaaattg tgcatgctcc agccaggctt
     6601 caagctgtcc tttcgttgct ccgctcaaat cagcggtagc cacaatgagt tgctcgcgga
     6661 catactgtaa atattgggta ttcatgcggc agctcctttc agtgttttgg cgtaattctt
     6721 cagtatccgg taatcggtca aaacagaacc aggaaaacga tataagcgca ggcgcatcca
     6781 gcggtggcga agacgttctg ccatataaga ctcaaacatc attcattccc cagttcggtg
     6841 atggtcagct ccagcttccc acctttggta acgggcatct tcacaacacg gtaatcaacg
     6901 acctgagcat catccagcca gaaacctgct ttggtgagtg cgtcaaaagc ggctttttgc
     6961 agattatcca ggtcacggcg acggcgatcc ggcatgtggc actcaatacg gattttcact
     7021 ggcatagcca ggccgatatc cagcatggag cctttgatga ttcgggcgac gttatcacgg
     7081 tatgcctgcc cttctgcgct gatgtgcgtg cgcccgcgat tatgccggta gtagcgatta
     7141 ttgctcggag gccagggtaa tgtgatgctg taggtattca cgccttaata accccctctt
     7201 tcagccagat aacctg
//
LOCUS       DAZKFZ010000225         7200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000225 DAZKFZ010000000
VERSION     DAZKFZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(190..396)
                     /locus_tag="ACHOQW_004138"
     CDS             complement(190..396)
                     /locus_tag="ACHOQW_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9575104.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(496..1347)
                     /locus_tag="ACHOQW_004139"
     CDS             complement(496..1347)
                     /locus_tag="ACHOQW_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFP9575105.1"
                     /translation="MKNWSVEHTREVKKWLDIDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1774..6360)
                     /gene="ypjA"
                     /locus_tag="ACHOQW_004140"
     CDS             complement(1774..6360)
                     /gene="ypjA"
                     /locus_tag="ACHOQW_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFP9575106.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSH
                     IGFDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYH
                     SNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELG
                     ATLSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKA
                     SFSSTLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(6425..6544)
                     /locus_tag="ACHOQW_004141"
     CDS             complement(6425..6544)
                     /locus_tag="ACHOQW_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9575107.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(6768..6926)
                     /locus_tag="ACHOQW_004142"
                     /pseudo
     CDS             complement(6768..6926)
                     /locus_tag="ACHOQW_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1776 a         1806 c         1567 g         2051 t
ORIGIN      
        1 cgtcaaataa acaggtacgg ctagtgggct gtatccatac ccgatattaa gaaaaccatc
       61 tctaagcctt atagcaaaag ctctgccaga aaaaacgtaa agaattacgt taaatataga
      121 tttttttaag atttaaactt tacaaatctg agaattaatc ctcataaata agaagactgt
      181 cattttattt caggcaacat gctttcttga attttcgata gttaattgaa tccattcgtc
      241 tacttcactt tcaatgaaag caacagagcg cgcaccgatt ttaacggggg aaggaaaacg
      301 attttggttg atgaggtgat agatccaagc tttaccgtat ccagttcgat gcagaacctc
      361 tggtaaacga ataaggcggg tgttgtgctg attcatagtc gtttcctctg tgccgttttg
      421 atggaaatga ctatacaaat gaatcagcag gaaaaagacc gaagctggga acgccagagc
      481 taatggaggt tggtcttagt cagaatcagc gattttcatc acgtctgaaa ctctgatctt
      541 tttcaaatgg ctgttcttgt tgatgaacag attaaactgg cgaataaaag gtatactcgc
      601 cgccttcaaa gcaagaggtt tgtctgtatc cctgatttgg tggttcaggc gagtgttgat
      661 ctcatcatcg tcatctgtat acaacaggcg agaaaggcgg tcttctgata tacgtacttt
      721 atataatttt gaccatacca gaatgtcgat catgggaagg agtctgtaat taatgatttt
      781 cttaatcgtt ccataaccaa agcgaatagc ttccgttaca tctggttcta tgccacgcac
      841 tttacgccat tgaggaagtg cagcctttag ggattcagcg atctcttcat ctgtcccgtt
      901 tgctaggtcg atttcgacca tgacagtatc cctgaacaat tttggcaaaa cttccgatac
      961 agggctatcg aggaattctc tgttaacaga atactcatcg cttccatccc aaaaaaacag
     1021 tgagttccgt aatccaacta tgctgagttg agccagtctt tcggttgtcg tcaaaaagaa
     1081 gtgcgggggc tggtaaagag tatcctctct tgtgagatag ccgagatgct tttctgtgat
     1141 gagaaagggc tttccagtga agatcctgtc gatgtagagc cttacggctt ctgcttcaaa
     1201 ttcttcatga tatggtttga agaacaatgt ccttgccagc aattcatggt agaggtgtat
     1261 aagcggaagc tcttcgaatc cgcgatatgt atctatatcc agccacttct tcacctctct
     1321 cgtatgttca actgaccagt ttttcataac gatctccaac aggcattaga cttaatgtat
     1381 gtagcaaacg taatcgacat gttaaaggaa gaccaatgaa atgagttcag atgccatcag
     1441 gaagttgaac caaaaggttt gatatttttt gatacagttc gctaacggac tattttcagg
     1501 ggtttatgcc gttacgattt atggttcctt tatagaacca aaggtaaagt cagaaacagc
     1561 agttatctag aaaattcaat caaatagata tctagttaca gggacgccag ccaatcatga
     1621 ttggacggtg taaggacaac accaacaaaa acaggaagtt agaagtctca gcaggacacc
     1681 gaccagacgg tgaggagaca taaaaggata cgcaaaggag ccgcggctcc tggtgacatg
     1741 aaagcccaca gatgtgggct ttttcgttga tggtcagaac gaccagttca caccagctac
     1801 cgcgttccac ggggattcca caccggcacc atggctatac cccaccccaa gatgcccgct
     1861 taacgtactg ctgaatgagg ctttaatacc tgcctggtat attccacgtc tgcccgacaa
     1921 atcattgacg aaattaccgt cattattcac cttcacccgg ttatcatcga caaattcttt
     1981 gcgcacagcc gccttcagcc acggctcaat ttccataccg ttccccagac gcatgttgta
     2041 actcagcgtt gcgcccagtt cacgatatat actgcgggta tcgactgatt tcgattccat
     2101 gccattggat aaatgatatt cggggttatc agcggtgaac cccgttaacg atgcatacgg
     2161 cgtcaggttc cagttaccat cggtaaatcg catcccggtt tcaatgtgac cgcccagccc
     2221 gttgctgtgg taactgccat tggcggctcc accgctgctc attttaccgg ctacgttact
     2281 ttcaaaacgg ttcagcttca cgacaccgtc cagatagaaa ccactttcat gttcccaact
     2341 ggcatagccg cccagagaat aactgcccac actgccatgt cctccgcgat caaaaccgat
     2401 atgtgaatgg gaataaccca taaaagcgcc cagcgtcgca atcccctcag gaatatcatt
     2461 agggctgtcg atccccactg tcattccggt cagcgtctgc tcaaacccgg cccccgcatc
     2521 ggtggtgaca ttattacggg tgttatacgt cgccccccag acattattgt tgtgtggact
     2581 cgctttcatt atgttcaacc gctcgcgaat actgtttagc tcagcatcaa ataccaacgg
     2641 taatgttgct gccatattga gtacggctgc ggtagaaggc gtgatgcgtt tctccggaac
     2701 gggtgtcggc gttggctcgg gagtcgggtc tggtttcgga tccggttttg ggtctggttt
     2761 tggatccggc ttcggatttg ggttgggatt tgggttggga tccgggttgg gtttgacatc
     2821 attggtcagg ttccagttgc tgttgccatc gcttttcagg acatactcat aggtcccaag
     2881 atcaacgaaa ccgccagtat tgcccagcga aaacgaagca tcccctcccc ctgttttcac
     2941 cagcgtcatc gcgtcgtcag actgaggact gacgccggta tcctgaacaa agattttaaa
     3001 attaccagtg gcgttgttgt tgacgaccag ttgatcgccc cgagagcctg caacgttggt
     3061 atgcaggtag aaattaccgc taccggaaag ttcattggtt gtcagcgtat tgtatatacc
     3121 gttagttgtg gcaggcgctg ctgtttgtgc tgataaataa acattaccgt cgttaagtag
     3181 caaactgttt actctatact cgcagttgct ggtgcctgca caggaattat tgctgttaag
     3241 ccagacactg ccccggctgg cagccgtcag gtcggcaagc gtcgtatcaa cgccattgcc
     3301 gatagttaat gtcgcgctat cggtaatccg gatcgcccct tcgagtttaa ctggcgtaac
     3361 attatcccgt ggcgtcatta acgacaggct tcctgtcgcg ccactgaccg atgcatccgc
     3421 cagcgtacct gcgtagacga ttgctgtccc gccagcaacc tggagatctt ctgcccgggc
     3481 cagagcctga aactcatctt ttgaccgccc aagggtatat tgcccaccag agttaacctt
     3541 tgtggcggag tcctgaccct ggttttgcat tgcccccccc ttgccaaccg tggagtcgcg
     3601 agcttcggta cctgctaata ccaataaatt accgccattt tccagcaaca tattggtcgc
     3661 taaattgccg gaaatggaaa aagtgccgta ctggtgagta ccgctgattt caataccgtt
     3721 agccgtgctc gtctggagag cggcaccgct gttctggacg atatctgtcg ctttgccatt
     3781 atcgttgact gtcagcgtac cgccttcatt gatcgttgtt tttattgcct ctccgttagc
     3841 tgaaactgtt tgtattccgc cgtcgttaat tgttgtctca ttcgccacac cctcgacaat
     3901 ttgttcaccg ccggtgagcg tcgtgcctgt cgcagtggct tttgttttga cgatctcccg
     3961 tccgcccgta ttgacctgtg ttttgtcaga agaggtgtct gactccacgg ttaacactcc
     4021 gccatttgcc agcaggatat tgttcgccgc accctgctcg atgctgaacg cgacgccatc
     4081 cgcgcgtgtt cctgtgaccc gcgtcgccct ggtggttgca accaacgcgc cctggctact
     4141 ctgctgtatc cccgttgcgc tgcctttctc ccgcacatcg agtgtgccgc catcattaag
     4201 caccgagttt tcagccagac cgccctcatt aactatctgt gaaccgccat taataatgga
     4261 accttccgct gtcccgtttg ccataatttg ttgtaggcca gagacgatat cggtattgat
     4321 cgccttacca taattctgaa cggtttgcgt gccaccattg atgtgtgttt tctctgttga
     4381 cccaccatca acaatttgtt caccactttc gatatttgct tcagtggcta aaccatatac
     4441 cgtttgcttg ccacctttga tatttgcttt atcagaagtg gcactggcat atattgtttg
     4501 ggtgccagca ctattgagta cagtgccaac atcttttcca taaacatcca ttttgccgtt
     4561 ggcattaata atcgtgtcaa ctgcccggga accagtgacg actgttaatg agccagcgtt
     4621 ttccagcact acatttttag cttctgaatt cctgatgtag aaagcatcac cataactgtt
     4681 ggttccttcg ataagtgttc cggaggtcgt ggaagcaatt aatgcgccgc cggattgttg
     4741 ctcaacatgc ttagcctcac caccgtcctg aacctccaga acgccgccat tattaagtct
     4801 ggtgctatct gttttagcct ccttctggac aatcagctta ccgccagcat caatggtagt
     4861 atttttcgcc gaggttttag ccactaccgt cagttcgccg gtattttcca gcacaacata
     4921 attagcctcc cctccggtaa tagtgaagtg agagagcttg ttgtatcctt cgatatcagt
     4981 ccctgcaccc gtgttggcaa ctaaagcact gcccgtctcc tggttaaccc catgtgcaat
     5041 accgccggta tagacaatca gcgagcctcc ggcgctaata ttgctgccaa ttgccgtacc
     5101 atctttctca acaacctgcc ggctcccgga ggatataatt gttgtgtcag ctttcccgcc
     5161 gcttttgata ttttgcgttc cgctgttgat attggtgcct gtggctatgc cataattatt
     5221 aatattctgt gtgccaccat taattatggt atttgtcgcg tttcctgcaa catccataac
     5281 cccgccatta tctattcggg tcgcatcagc tttagcattg gttaaaactg acattgttcc
     5341 tttatcttta ataatcgtct tgtttgccga accatatgcg tttatgtcta aatgaccacc
     5401 gttttccagc aacacattgt ctgccacgtg attgtggatg gagaatgcac cttcactatt
     5461 cgtaccgctc accgtcgtac cgttagtgtt agtttttaaa attgcaccat cgtgctgggt
     5521 aacatttgtt gccgtaccac cactaacatc aagcacgcca ccggaataaa cttcaataac
     5581 atccgaggag ctggtgttat caataatttg cgtaccacca gaatagatct gagtattttt
     5641 tgccgttgac ttactattca gagactgagt tccgccttca atcgtcgtgt ccagcgcacg
     5701 gctctcatat actctttgct caccgccatt tttgatggtg gttgtttcta ctgtgctctg
     5761 ttcaacatac tgtcgaccac catttatggt tgtgttcgtt gccagacttc cttgtactac
     5821 gtcctgagag ccagacttat ttatcgttgt accatcagca tgcccttgaa cttttactat
     5881 ctggctacca ccatcaatga gtgttccatt cgcactccct ccctctacgc gtgaagcacc
     5941 gcccttaatt gtcgttccat tgctgatacc ccctttataa acgtcctgat tgccactctc
     6001 gattatcgta cctgtggaaa tacccccgtc atgaattgac tgtctgcccc cgttaatggt
     6061 ggtattatta gactgcccca cataattgtt atgccttcct acatcttgat atccaccaga
     6121 ttcaataaga cttccattag ataccccgcc ataaacattc tgctggccat ggttgataat
     6181 atgagtgtta tttgttgtac ctcgttcatc tactcgttgg ctgccatcta cagtctcatc
     6241 gtttaccaca ccaataacat caggagtgaa ggcagccatc ccgggcgggg catatatcaa
     6301 ggcagatatc aataaggaaa gtactgagcg acgacaataa tagggactgg tcctgttcat
     6361 aaatttcatc ctctgaaaag tgaatactga gtagcgttta agcgacctta gctttgctgc
     6421 aacatcagcc cacaggcacc agaccagggg attcatcctg aagagacagc gcaagtgtat
     6481 tgtgttcacc gctcatcaaa gacatcatga tgaaatgatg atattccgca taagaatgag
     6541 gcatttttta aacgcagtgc gctgaagtgt ggttggataa aaaagtcaat ccattcagga
     6601 aatacgggcg tattcttttc tttcgacaat gaggccgttg gcaaaataaa atgatttaca
     6661 taatcgtttc tgatgaatat cttctgctca cataaaaatc acacaataac tttgagatcg
     6721 cagattgttt tacttttaca gcattcgtcc ccccattgtt gggcaaatat agattgggcc
     6781 agagcacgaa agttaatacc acgttcgcac agctcctcca acagcacgac aagatgccac
     6841 atactgcgcc ccagtcggtt cagtttacag actagcagtg ctttccctgc cgatgatgtc
     6901 ctgagcagtt ttttcagtcc ggacctcctg ccacggtttt acgttgaagc ggtaaccctg
     6961 agcacgcagt tcttcagtca ggcgtggtgc accgtaacgc tgttattgct gggtgagatc
     7021 aaaaaacttt caggcagcta aggaaagttg aaccagacaa tagatgaaat atttcaacca
     7081 attacagcac caattcagtc actgccagcc caccaaataa atcaaggggt tacatgaaaa
     7141 cgtagcccct ttttctttgg tagtgacact aaaatggatg tagtgtgaag aataatcccg
//
LOCUS       DAZKFZ010000226         7157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000226 DAZKFZ010000000
VERSION     DAZKFZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(121..1011)
                     /gene="metF"
                     /locus_tag="ACHOQW_004143"
     CDS             complement(121..1011)
                     /gene="metF"
                     /locus_tag="ACHOQW_004143"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP9575108.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            1087..1221
                     /locus_tag="ACHOQW_004144"
     CDS             1087..1221
                     /locus_tag="ACHOQW_004144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575109.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(1360..3792)
                     /gene="metL"
                     /locus_tag="ACHOQW_004145"
     CDS             complement(1360..3792)
                     /gene="metL"
                     /locus_tag="ACHOQW_004145"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP9575110.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3795..4955)
                     /gene="metB"
                     /locus_tag="ACHOQW_004146"
     CDS             complement(3795..4955)
                     /gene="metB"
                     /locus_tag="ACHOQW_004146"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9575111.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            5232..5549
                     /gene="metJ"
                     /locus_tag="ACHOQW_004147"
     CDS             5232..5549
                     /gene="metJ"
                     /locus_tag="ACHOQW_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP9575112.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5609..6217
                     /gene="yiiX"
                     /locus_tag="ACHOQW_004148"
     CDS             5609..6217
                     /gene="yiiX"
                     /locus_tag="ACHOQW_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP9575113.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            6362..7037
                     /locus_tag="ACHOQW_004149"
                     /pseudo
     CDS             6362..7037
                     /locus_tag="ACHOQW_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1749 a         1981 c         1799 g         1628 t
ORIGIN      
        1 aagtaatgag ggagaaaaca aggctgataa ccttattttc ccgccctcat ttcgaggcag
       61 cattttgtgc tctgtttaaa atttgtgatc actgtgtgat tttcataaaa gccacactat
      121 ttataaacca ggtcgaaccc ccagcgtatg gcaaatcgcg taactcattt cagcgcggtt
      181 aagcgtatag aagtggaaat ctttcactcc ttcacggctt aaaatcttca ccatatccat
      241 ggcaatattc gcgcccacca gcttgcgggt ttcggcatcg tcatccagcc cgtcaaacat
      301 ttgcgccatc cacgccggaa tacgcacgtt ggtcatgtcg gcaaatttct tcgcctgttt
      361 aaagttagat accggcaata tgcccggaat gatttccaca tcaatgcccg ccgacacaca
      421 gcggtcacga aaacgcaggt agctttcgac atcaaagaag aactgagtaa tcgcgcggtt
      481 ggctcctgca tcgactttgc gtttcaggtt aagcaaatcc gcctgggcgc tttttgcttc
      541 cgggtgaact tccggatacg ccgccacgga gatatcgaaa tctgccactt cttttaatag
      601 cgtcaccagg tcagaagcat acatttctgg cttaccactt cccggcggca gatcgccacg
      661 cagcgccacg atatgacgaa taccgttatt ccagtagtcg cgtgcaatgg tgcgcaactc
      721 gtcgggcgtc gcatcaatgc aagtaagatg cggtgccgct tccagaccag tgcgatcttt
      781 aatgccttta ataatgctgt gcgtacggtc gcgctcgccg gagttcgcgc cataggtcac
      841 cgatacaaac ttcggtttca ggctgctaag gcgatcgatg gagttccaca gggtctgctc
      901 catttcactg gtacgcggcg ggaaaaactc gaacgaaacg ttaatctgcc cctggacttc
      961 tgccaggctc tgattcaggg catcccgctg gctggcgtga aaaaagttca taccttacct
     1021 catcaatcgc ttgtagttat atgttgtgtt gtgcacatcc atccgtttag acgtccagat
     1081 gtaaagatga aggaaaagcc gaagggcgtc aaccgaaaaa tgaccaatga tggtgaggaa
     1141 aaatcaggga agatgaaaaa acttcaggat ggtaagaaaa agaaaatccg gtatgcaaag
     1201 caaaccggac tgcaaatata aactaaccat gtacttattg cttcttgccg gattcgtcgt
     1261 aaacgcttca tccggcctaa tcctatgata attcaatata tttcattaga aaccataaac
     1321 ccgaaaacat gagtaccggg cattattaaa tttctgaaat tacaacaact gtgccagccg
     1381 gttgatatcc gactgaatcg ccccggcggt gacgtcgcgc cccgcgccag gcccacggat
     1441 caccagcggg ttgtcgcgat accagcggct ttcgatggca aagacgttat cgcacggtag
     1501 cagtgatgcc agcggatggt cttcacgcac cgcctccacg cccacacgcg ctttgccgtt
     1561 ggcatcgaaa cgcgccacgt agcgcagcac cagccccatt tcgcgggccg cttccagccg
     1621 ttgcaccatc tgctcgttaa gttcatcgcc attttcaaag aaatggtcga tgctgccgcc
     1681 ttcgcaatga gcaggcacca gcgattccac acgtacctgg tccggttcaa tgttgtaacc
     1741 cgcttcacgc gccagaatca ccagcttgcg cattacgtct ttgccggaga ggtcgtcacg
     1801 cggatcaggc tcggttaagc cctgctgcca cgcctgatcc accagctcgg taaacggcac
     1861 gctaccgtcg aattgcagga acagccagga gagcgtgccg gagaagatcc cactgatcga
     1921 caaaatagta tcgccgctgt cgatcagatc gcgcacggta tggttgatcg gcaagcccgc
     1981 accgacggtg gcgttgtata gccagtgacg cccggttttt tcgaaggcgt cgtggatctg
     2041 gcgatattta ttgctgtcgc ttgctcccgc cagtttgttg gcactgatga cgtggaaacc
     2101 gtggctggcg aaatcaagat actggtcagc cagctgctgg ctggcggtaa cgtccagcac
     2161 cactaaatca tcatacggat gggcgcgcat ccacaggaac aacgactctt catcctgctc
     2221 aaccgcttca tcgttgaaga aggctaacgc gcggctggcg tccagcccgt cataactcaa
     2281 cagactgcgg cggctgtcca ccacgcctgc cagcacaaat tcaaagccgg tacgcgccga
     2341 aagcgtgctc tgctcgcggg cgaacagttc cagccagcgg gaaccgatat tgcctttgcc
     2401 gaacaatacc aggccgatgc gtttttctgc gcggaagacg gactgatgca ggccctgaat
     2461 caggctttcg gtcgggccgg tgcgcagtac tgccaccagg ctgatgccgt catcggactg
     2521 ccaggtaaac tcgaccggct ggcctttcag ttgctgccag aagcggtggc aatgcagcgg
     2581 gttacgggtg acacccgccc cgaccatcgc caccagcgcc agcccctgac gcaggcgcag
     2641 ttcgccaggt aatcccgctt cgtcgaggat tttcagcgca ctgtcggcca cttctgaggt
     2701 gtagcaaaat tgcagcaact ggcgatcgtt atgcacgcca accgccagtg ggcgcacctg
     2761 agcacgtttc aggatctggt cgatctcttt atgcgccagt ttgaaatcct ggctggtggg
     2821 cacctgaaac tcaatcaagc agacatcatc gtggctggtg acaatacgcg caccagtacc
     2881 ggaggccagc acgcgttcaa tgcgcgtgga gccttgatcc ggcgtgtagc tacagcgcag
     2941 ctgcaggtcg atttcgctac cggatactgg ctgtaaagta cgggcgtgaa gaacgggagc
     3001 cgccagacgc gccagttcgc tggcttcatc cagacgcagc aatggcagca gacaggcatc
     3061 tttcacttta cgcggatcgg cactgtatac cccggcgacg tcgctccaga tggttacacg
     3121 agaaacaccc gccagcgcac cgatttgtgt cgcggaatag tcggaaccgt tacgccccag
     3181 cagcaccgtt tcaccggcgt tgttacggct gataaacccg gtcaccacca gacgtttacc
     3241 cggatgttgt accagtagct gttgcagcaa cgggtaagaa agcccttcat caacctgcgg
     3301 ttgcgcagcg cgttcagcgc gtaaaaactc gcgggcatca agccaggccg ctggcagccc
     3361 ttgttgatta agcaccgcag acatcaggcg cgccgaccac acttcgccgt ggcccaccac
     3421 ttccgcatac actgcgtcgt taataccgct gtcgagcagc gccgccaggc gctcaagatc
     3481 gctgacaaaa gcgctaatga ggctatcggc ttcttcggcg ggtagcagac cgctaatcag
     3541 atcgcactga tagcgacgca gcgtttgttg aacctgatgc gcagagagac gatcggtctg
     3601 gcttagtttc aaccagttaa tcaactggtt agtggtgcta ccagcggcgg aaaccaccat
     3661 catatcgtca ggctgagagt actccgccat aatgcccgcg acacgcaaat aacacttcac
     3721 atcagccaga ctactgccac caaatttatg cagctgacga cctttcgccc ctgcctgcgc
     3781 aatcacactc atttttaccc cttgtttgca gcccggaagc cattttccag gtcggcaatt
     3841 aaatcttcgc catcttcaat accggtggag atacgcagca gcgtctcgga gatcccggcg
     3901 gcagcacgcg cttctggtgc catgcctgca tgtgtcatgg ttgcggcgtg agagattaaa
     3961 ctttccactc cccctaatga ttccgccagc gtaaataacg acagcccgcc caggaaacgg
     4021 cgcagcgtct gctcatcgcc atccagttca aaactcaaca ttgcgccaaa gcctttttgc
     4081 tgacgcgcgg caatttcatg cccctgattt tccggcagcg acgggtgata cagttttttc
     4141 accaacggct gggtttgcag atatttcaca atcgccttcg cgttgcgctg cgccagctcc
     4201 atacgcggca ccagcgttcg caacccgcgt agcagcagat agctgtcaaa cgcgccgccc
     4261 gtcacgccaa tattgtttgc ccaccaggca agttcagtga caacgtccgg gtctttagca
     4321 atcaccacgc cagccaccac gtcagagtga ccgttcagat atttcgtaca tgaatgcaac
     4381 accagatcgg cacctaatgc cagcggattt tgtaatgccg ggcttaagaa ggtgttatcc
     4441 accacgctca ccgccccgac ttcccttgcc agatggcaga ttttcgcaat atccacgacg
     4501 cgtaacaatg gattacttgg gctttctacc agtaccagtt tggggttttc tgccagcgct
     4561 gcccgtaatg cctgttcatc gccttgatca acaaacaaca cgcgatagca accgcgtttc
     4621 gccaggctgt cgaacaggcg atagctaccg ccgtagcagt cgtgcggcgc aaccagcaga
     4681 tcgccaggtt tcaaaaagac ggtcgttacc agatgaatcg cggacatgcc ggtattagta
     4741 agtactgcac cagcaccgcc ttccagttct gccagcgcac gctgaaccac atcgcgcgtt
     4801 gggttgccgc gacgcgagta atcatgcgcg cgcggttcat taaatccggt aaagttatag
     4861 gtgctggaaa gatggatggg agggacaacg caaccatact gttcgtcgtc atttaacccg
     4921 ctacgcactg cgatggtggc ctgtttacgc gtcatgtgat gaagttccct gggcttggtc
     4981 ggtgaaatgt caggcaccag agtaaacact gtgttaatgg acgtcaatac atctggacat
     5041 ctaaacttct ttgcgtatag attgagcaaa tcccaaatag ccgttaaaat tatatgcatt
     5101 atcacgccga caggtgcatt acacgatgtc acggtaacgc ctgtacggta aactatgcgg
     5161 gtttacggtc agtacccaca taaattgtgt ggtctggtct caatttattg acgaagagga
     5221 ttaagtatct catggctgaa tggagcggcg aatatatcag cccatacgct gagcacggca
     5281 agaagagtga acaagtcaaa aagattacgg tttccattcc tcttaaggtg ttaaaaatcc
     5341 tcaccgatga acgcacgcgt cgtcaggtga acaacctgcg tcacgctacc aacagcgagc
     5401 tgctgtgcga agcgtttctg catgccttta ccgggcaacc tttgccggat gatgccgatc
     5461 tgcgtaaaga gcgcagcgac gaaatcccgg aagcggcaaa agagatcatg cgtgagatgg
     5521 ggattaaccc ggagacgtgg gaatactaaa gcggaaaaga gcggcgcgga gttaaccagc
     5581 cgcttttttc atacaaggac gtttaacgat gaaaatcagg ctactgatcc tcagcctgct
     5641 ggtttctgtg cctgcctttg cctggcagcc gcaaactggc gacatcatct ttcaaatctc
     5701 tcgctcatcg caaagtaaag cgatccaact ggcgacccat tccgattaca gccacaccgg
     5761 tatgctggtg atgcgtaaca aaaagcccta cgtttttgaa gcggtcggcc cggtgaaata
     5821 caccccgctc aagcagtgga ttgcccatgg tgaaaagggc aaatacgttg ttcgccgcgt
     5881 tgaaggcgga ctgagtgttg aacaacagca aaaactggcg caaacggcga aacgttatct
     5941 cggcaaaccg tacgatttca gcttctcatg gagcgacgat cgccagtact gttcggaagt
     6001 ggtgtggaag gtttatcaga atgcgctggg aatgcgtgtg ggcgaacagc agaagttgaa
     6061 agagtttgat ctcagcaacc cgctggtcca ggcgaagctc aaagaacgct acggcaaaaa
     6121 tattccgctt gaggaaacgg tggtctcacc acaagccgtt ttcgatgcgc cacaactcac
     6181 cacggttgcc aaagaatggc cgctgttttc gtggtaatgg atgatgaaaa aaataattcc
     6241 cgccgtagtc tgtttgttgc tttgtagttg cacgaccttt ccgcaagctg tcgctccggt
     6301 aaacggtgat gcctccaatt agttgaatta aggatataat actcgctttt aaggatgttt
     6361 catggtcaca gttaaccttc attgtccccg ttgccagtcc gttcaggttt accgtcgcgg
     6421 gcagaacccc aaaagacatg atcgattccg cggccgtgac tgtcaccggg tatttcagct
     6481 cagttactgt tacgagcccc gtaaaccggg agttaaagac caaatcgctg aaatggcttt
     6541 caacggtgcc ggggttcgct atactgcccg tacgctgaaa atcggcatta atacagtcat
     6601 ccgaacttta aaaaactctc gccacgaaga ataacgtcat ccccggtcgc tcatgctgat
     6661 gtggcactca tctgcaaact tagtcagcaa tggagctttg tcggcagtaa agcgcgacag
     6721 cactggctct ggtacgtgta caacacgaaa actggtggtg ttctggccta cacttttggc
     6781 ccccggactg atgaaacctg tcgtgaattg ctggcgctgc ttaccctttt accatcggca
     6841 tgttgaccac tgacgaatgg ggtagttacg ctagagagtt accaaaggag aagcatctga
     6901 ctggtaagat ttttactcag cacattgagc gtaacaatct gaccctgaga acccgaatca
     6961 aacgtctggc ccgcagaacc atctgtttct cgagttctgt agaacttcat gaaaaagtca
     7021 tcggtgcttt tatcgaaaat atatgttcta ctaattggag tcatcaccct tctgatgagc
     7081 tttccttgga tgattaccag actgaataca cagccaaaga tttttacctc attctgctct
     7141 tttttctcaa atgacat
//
LOCUS       DAZKFZ010000227         7144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000227 DAZKFZ010000000
VERSION     DAZKFZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(334..1470)
                     /locus_tag="ACHOQW_004150"
     CDS             complement(334..1470)
                     /locus_tag="ACHOQW_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9575114.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1473..1835)
                     /locus_tag="ACHOQW_004151"
     CDS             complement(1473..1835)
                     /locus_tag="ACHOQW_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9575115.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2372..4285
                     /gene="mtlA"
                     /locus_tag="ACHOQW_004152"
     CDS             2372..4285
                     /gene="mtlA"
                     /locus_tag="ACHOQW_004152"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP9575116.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4380..5528
                     /gene="mtlD"
                     /locus_tag="ACHOQW_004153"
     CDS             4380..5528
                     /gene="mtlD"
                     /locus_tag="ACHOQW_004153"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9575117.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5528..6115
                     /gene="mtlR"
                     /locus_tag="ACHOQW_004154"
     CDS             5528..6115
                     /gene="mtlR"
                     /locus_tag="ACHOQW_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP9575118.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6127..6336)
                     /gene="yibT"
                     /locus_tag="ACHOQW_004155"
     CDS             complement(6127..6336)
                     /gene="yibT"
                     /locus_tag="ACHOQW_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP9575119.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6621..6983
                     /locus_tag="ACHOQW_004156"
     CDS             6621..6983
                     /locus_tag="ACHOQW_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP9575120.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1823 a         1789 c         1806 g         1726 t
ORIGIN      
        1 ctacattgtg atctatagat taaatatttt ttaaatgtga ctgtcatcct tcagataata
       61 aagccatcat cgcaatagat ggaaaagcac tcctgctctt ttttgattag agccgtcgca
      121 ggaggaacaa ctcatatcat cattgcgatc tcgacgatgt atagtctcat cctgcggcag
      181 aacaagacgg ataaaaaatc caatgagagc acactcatcc agaacttatt aacataatga
      241 atattaaaga aaaatcatcg cagctaatga agtaatatgc aaaaataatt agtccttaca
      301 aggagcaaag ttccataatc tcccccctcc ccctcaatga tccaaataaa gataatgcat
      361 ccagctggtc attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga
      421 gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca tcgatatcat cgttagggtc
      481 cagttcaatg gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac
      541 cccctgcgcc tgataagaac cgcctggcac gacaggtaaa atactagtca gtttgccgtg
      601 aaacacctgc ccaggtagcg cgttaaacac cacttccgca tcatcgccgg gtttcagacg
      661 taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaagac
      721 catcaccgga cgcagcggca aggcagctgc gtatgtacct gggcggatca gtacctgagt
      781 aacgtagcca ttgctcggcg cgcgaatgac agtctgctca aggttatatt ttgcttcagt
      841 aagttgcgct cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg
      901 gctctggatc tgcgcctgct ccgccaccga gcctttcacc agcgcatcct gcgcgaggaa
      961 attttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc
     1021 tttcaagtaa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt
     1081 gttggcctgc gcttcagtga gctgcgcacg cagcgtcttt atattatgcg tcgccgtcat
     1141 caggtcagcc tgaagtctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag
     1201 cacctcgccc ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt
     1261 cacctgtggc gtgataggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg
     1321 gttgtagttc atcaataaaa tcaaaccact caccagaaac acgcctccca gcgccgccgt
     1381 cgccagcgtc cactgattta ccggaatgcg gaagatttta aagaccgccc acgccagcgc
     1441 cacgtaagtt aaaacaatca atagatccat aattagatct ccgggaacgt ggaatagtca
     1501 gtctttttct cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgaggcg
     1561 atttccgggt cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc
     1621 tccggttgat agagcgtcgc ccagatccac agaaacggcc agataacatg cagagtaaac
     1681 aggctcaccc agcccgccgt atgaatagcg tcggcatggg ggtgattgcg ctttttggca
     1741 atcagataag ggatgtcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag
     1801 atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca
     1861 gtgttgtttt tgggctggca gccagaaggg agtcaggctg atattttgac aataatccgg
     1921 gttcgcgatt ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt
     1981 acataacgat tgtatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta
     2041 agaaaatatc ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga
     2101 cactactcac atttaaatgc catttttagc gaaaatcgcc gccttgttgc ttttttacac
     2161 aagcgttttg tgatgaacgt cacgtcaatt acctctctac cccctatatt tatgtgattg
     2221 atatcacaca aaaggccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta
     2281 tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg
     2341 cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca
     2401 aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg
     2461 gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaga cgctggcgaa
     2521 gctggtcggg ccgatgatca cttatctcct gccgctgctg atcggttata ccggtggtaa
     2581 gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatc accaccatgg gcgttatcgt
     2641 cggcgcagac atgccgatgt tcctcggttc tatgattgca ggtccgctgg gcggctggtg
     2701 cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt
     2761 gaataacttc tccgcaggca tcatcgggat gatcctcgct attctggcat tcctcggcat
     2821 tggcccgatt gttgaaaccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt
     2881 ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa
     2941 caacgccatt aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg
     3001 taaatcaatc ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg tgctgctggc
     3061 gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg caatcatcca
     3121 cttcctgggt ggtatccacg aaatctactt cccgtatgtg ctgatgaatc cgcgtctgat
     3181 cctcgcagtc atcctcggcg gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg
     3241 tctggtttct ccggcatctc cgggttctat ccttgctgta ctggcgatga caccaaaagg
     3301 tgcttatttc gctaacatcg cgggtgtgtg tgcggcgatg gctgtctcct tcgttgtctc
     3361 tgctattttg ctgaaaacca gcaaagtgaa agaagaagat gatattgaag cagcaactcg
     3421 tcgtatgcag gacatgaaag ctgagtctaa aggtgcatct ccgctgtctg cgggcgatgt
     3481 gactaacgac ctgagccacg tacgtaaaat catcgttgcc tgtgacgccg gtatgggttc
     3541 cagtgcgatg ggcgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc tgtcacagat
     3601 ttcggtcact aacagcgcga tcaacaacct gccgccagat gtggacctcg tcatcactca
     3661 ccgtgacctg accgaacgcg ctatgcgcca ggttcctcag gcacagcata tttcgctaac
     3721 caacttcctc gacagcggcc tgtacaccag cctgactgaa cgtctggttg ctgcccagcg
     3781 acatactgaa aacgaagtga aagtgaaaga cagtctgaaa gacagctttg acgattccag
     3841 tgctaacctg ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag cagcaaccaa
     3901 agaagaagcg attcgttttg ctggcgagca gctggtgaaa ggcggttacg ttgagccgga
     3961 gtacgttcag gcgatgctgg atcgtgaaaa actgaccccg acttatctgg gtgagtctat
     4021 cgcggtgcca cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg gcgtggtgtt
     4081 ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg ctcgtctggt
     4141 gattggtatt gctgcccgta acaacgagca cattcaggtt atcaccagcc tgaccaacgc
     4201 actggatgat gagtccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct
     4261 ggaactgctg gcaggtcgta agtaatccaa tcctaccctc tcctcacgga gagggtttgg
     4321 gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta
     4381 tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg
     4441 cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga
     4501 atgcccgtca tagctatcag gttcatgtgg tcggtgaaac cgagcaggta gataccgttt
     4561 ccggcgtcga tgctgtcagc agcattggtg atgatgtcgt tgatctgatt gctcaggtcg
     4621 atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg gctatcgcca
     4681 aagggctggt gaaacgtaaa gaacaaggta atgaatcccc gctgaacatc atcgcctgtg
     4741 aaaacatggt acgcggtacc acgcagctga aaggtcatgt gatgaacgcc ctaccagaag
     4801 acgccaaagc gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt gaccgcatcg
     4861 taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa actttcagcg
     4921 aatggattgt cgataaaacg cagttcaaag gcgcactgcc gaacatccca ggcatggagt
     4981 taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg
     5041 ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgac gctattctcg
     5101 acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgcg gtactgatca
     5161 agcgctacgg ctttgacgca gacaaacatg cggcgtacat ccagaaaatc ctcggtcgtt
     5221 ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg ctgcgtaaac
     5281 tgagtgctgg cgaccgtctg atcaagccat tgctcggtac gctggaatac agccttccgc
     5341 acaagaatct gattcagggg attgctggtg caatgcactt ccgcagtgaa gacgatccgc
     5401 aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg ctggcacaga
     5461 tttccgatct tgatgccaac agcgaggttg tatccgaggc ggtaaccgct tataaagcaa
     5521 tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat
     5581 gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc gtctgaatgc
     5641 tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg aggcggtaaa
     5701 tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg ctgtagaacc
     5761 gttactcgac ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg
     5821 gttgggcgtc attaaccgcc aggaatacga agatgcggag ctgctgatgg cattgcgtga
     5881 agaactaaat cacgacggca acgagtacgc ctttaccgac gacgagatcc ttggaccctt
     5941 tggggaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc
     6001 cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct
     6061 ttcactgact gagctgattt ccagaatcag cttaaaaaaa gcctttcaaa agtaagcaac
     6121 gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat actccagacg
     6181 ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg
     6241 aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc
     6301 gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc
     6361 gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga cattaaggaa
     6421 aacgtgctga atcctcaata atcatccgct gctgtttatt atcttttttc tatagatttc
     6481 ttaattaatc aataaattgc gtagttttta agcatgttaa tgattacgtt acagaactat
     6541 tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta
     6601 tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg
     6661 atcgtctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa gccgcagaca
     6721 aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg
     6781 aagttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgtttcagc
     6841 gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac
     6901 tggtggttgt gcacccaatg accgcgctgg gccgtgaaat gggcctgcag gagatgactg
     6961 gattctctaa gactgcgttt taagcgcctc agacgactgg ttcgatcata tcaacccgca
     7021 tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa
     7081 caattcactc cctttacgtc gatttaccgc tggttgatat tctgaaaatg catttttacc
     7141 gaaa
//
LOCUS       DAZKFZ010000228         7076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000228 DAZKFZ010000000
VERSION     DAZKFZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            410..1399
                     /gene="araF"
                     /locus_tag="ACHOQW_004157"
     CDS             410..1399
                     /gene="araF"
                     /locus_tag="ACHOQW_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP9575121.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1469..2983
                     /gene="araG"
                     /locus_tag="ACHOQW_004158"
     CDS             1469..2983
                     /gene="araG"
                     /locus_tag="ACHOQW_004158"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP9575122.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            2998..3984
                     /gene="araH"
                     /locus_tag="ACHOQW_004159"
     CDS             2998..3984
                     /gene="araH"
                     /locus_tag="ACHOQW_004159"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP9575123.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4151..4951
                     /gene="otsB"
                     /locus_tag="ACHOQW_004160"
     CDS             4151..4951
                     /gene="otsB"
                     /locus_tag="ACHOQW_004160"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP9575124.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4926..6350
                     /gene="otsA"
                     /locus_tag="ACHOQW_004161"
     CDS             4926..6350
                     /gene="otsA"
                     /locus_tag="ACHOQW_004161"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP9575125.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6357..6785)
                     /gene="uspC"
                     /locus_tag="ACHOQW_004162"
     CDS             complement(6357..6785)
                     /gene="uspC"
                     /locus_tag="ACHOQW_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP9575126.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1785 a         1643 c         1830 g         1818 t
ORIGIN      
        1 tgaatgacgt gcattttcca catctttgag ttgcggttat tacaccattt caaaaactca
       61 acgccaggta atgcggccta ttgactggtg aaaaagaaga catcccgcat gggtaccaaa
      121 gacaacaagg atttccaggc taatcttatg gattaatctg ctgtgcattc aacaatttgt
      181 ctgacaaatt ggcttttcct tatgtctttt cccgctaaat ttatgcacgt tctcactgta
      241 attctgcgat gtgatattgc tctcctatgg agaattaatt tctcgctaaa actatgtcaa
      301 cacagtcact tatcttttag ttaaaaggta atgctttgtt ttccgattaa tttaacgaat
      361 gtcattcgtt tttgccctgc acaaaacgac actaaagctg gagagaacca tgcacaaatt
      421 tactaaagcc ctggcagcca ttggtctggc agccgttatg tcacaatccg ctatggcgga
      481 gaacctgaag ctcggtttcc tggtgaagca accggaagag ccgtggttcc agaccgaatg
      541 gaagtttgcc gataaagccg ggaaggattt agggtttgag gttattaaga ttgccgtgcc
      601 ggatggcgaa aaaacattga acgcgatcga tagcctggcc gccagtggcg caaaaggttt
      661 cgttatttgt actccggacc cgaaactcgg ctctgccatc gtggcgaaag cgcgtggcta
      721 cgatatgaaa gtcattgccg tggatgacca gtttgttaac gccaaaggta agccaatgga
      781 taccgttcct ctggtgatga tggcggcgac caaaattggc gaacgtcagg gccaggaact
      841 gtataaagag atgcagaaac gtggctggga tgtcaaagaa agcgcggtga tggcgattac
      901 ctccaacgaa ctggatacgg ctcgtcgccg tactacgggt tctatggatg cgctgaaagc
      961 tgcaggattc ccggaaaaac aaatttatca ggtacctacc aaatctaacg acatccccgg
     1021 ggcatttgac gctgccaact caatgctggt tcaacatccg gaagttaaac attggctgat
     1081 cgtcggtatg aacgacagca ccgtgctggg cggcgtacgc gcgacggaag gtcagggctt
     1141 taaagcggcc gatatcatcg gtatcggtat taacggtgtg gatgcggtga gcgaactgtc
     1201 taaggcacag gcaaccggct tctacggttc cctgttgcca agcccggacg tacatggtta
     1261 taagtccagc gaaatgcttt acaactgggt agcaaaaggt gttgaaccga ccaaattcac
     1321 cgaagttacc gacgtggtgc tgatcacgcg tgacaacttt aaagaagaac tggagaaaaa
     1381 aggtttaggc ggtaagtaat ttgccggaaa aattcccctt tgcatgatgc agagggggtg
     1441 tgaacgacca gtgattcacg gagacgttat gcaacagtct accccgtatc tctcatttcg
     1501 cggcatcggt aaaacgtttc ccggcgttaa ggcgctgacg gatattagtt ttgactgcta
     1561 tgccggtcag gttcatgcgt tgatgggtga aaatggcgca ggaaaatcaa ctctcttaaa
     1621 aatcctcagc ggcaactatg cgccaaccac gggttctgta gtgattaatg ggcaggaaat
     1681 gtccttttcc gacacgaccg cagcacttaa cgcgggtgtg gcgattattt accaggaact
     1741 gcatctcgtg ccggaaatga ccgtcgcgga aaacatctat ctcggccagc tgccgcataa
     1801 aggcggcatt gtgaatcgct cattgctgaa ttatgaggcg ggtttacaac ttaaacatct
     1861 tggtatggat attgacccgg acacgccgct gaaatatctc tccattggtc agtggcagat
     1921 ggttgaaatc gccaaggcgc tggcgcgtaa cgctaaaatt atcgcctttg atgagccaac
     1981 cagctccctt tctgcccgcg aaatcgacaa tcttttccgc gttattcgtg aactgcgaaa
     2041 agaggggcgg gtgatcttat acgtttctca ccgtatggaa gaaatatttg ccctcagcga
     2101 tgccatcact gtctttaaag atggacgtta tgtcaaaacc tttaccgata tgcagcaggt
     2161 tgaccacgac gcgctggtgc aggcgatggt cgggcgcgac attggcgata tctacggctg
     2221 gcaaccgcgt agttatggcg aggagcgcct gcgtcttgat gctgtgaaag caccaggcgt
     2281 gcgtacgcca ataagtctgg cggttcgcag tggtgaaatt gttgggctgt ttggtctggt
     2341 aggggcgggg cgtagcgaat taatgaaagg catgtttggc gggacgcaaa tcaccgccgg
     2401 tcaggtttat atcgaccaac agccgatcga tattcgtaaa ccgagccacg ccattgccgc
     2461 aggcatgatg ctctgcccgg aagatcgcaa agcggaaggc attattcctg tgcactccgt
     2521 tcgcgacaat atcaacatca gtgccagacg taaacatgtg ctcggcggtt gtgtaatcaa
     2581 caacggttgg gaagaaaaca atgccgatca ccacattcgt tcgctcaaca tcaaaacgcc
     2641 gggcgctgag caactgatta tgaatctctc aggtggaaat cagcaaaaag ccattctggg
     2701 ccgctggtta tcggaagaga tgaaggtcat tttgctggat gaacctacgc gcggcattga
     2761 tgttggtgct aagcacgaaa tatataacgt gatttatgcg ctggcggcgc agggtgtggc
     2821 ggtgctgttt gcctccagcg acttacctga agtcctcggc gttgccgacc ggattgtggt
     2881 gatgcgcgaa ggtgaaatcg ccggtgaatt gttacacgag caggcagatg agcgtcaggc
     2941 actgagcctt gcgatgccta aagtcagcca ggcagttgcc tgagtaagga gagtatgatg
     3001 tcttctgttt ctacatcggg ttctggcgca cctaagtcgt cattcagctt cgggcgtatc
     3061 tgggatcagt acggcatgct ggtggtgttt gcggtgcttt ttatcgcctg tgccattttt
     3121 gtcccgaact ttgccacctt tattaatatg aaagggttgg gcctggcaat ttccatgtcg
     3181 gggatggtgg cgtgtggcat gttgttctgc cttgcttccg gtgactttga cctttctgtc
     3241 gcctccgtaa ttgcctgtgc gggtgtcacc acggcggtgg ttatcaatct gactgaaagc
     3301 ctgtggattg gcgtggcagc ggggttgctg cttggcattc tctgtggcct ggtcaatggc
     3361 tttgttatcg ccaaactgaa aataaatgct ctgatcacga cactggcaac gatgcagatt
     3421 gttcgaggtc tggcgtacat catttccgac ggtaaagcgg tcggtatcga agatgaaagc
     3481 ttctttgccc ttggttacgc caactggttc ggtctgcctg cgccaatctg gctcaccgtc
     3541 gcgtgtctga ttatctttgg tttgctgctg aataaaacta cctttggtcg taataccctg
     3601 gcgattggcg ggaacgaaga ggctgcgcgt ctggcgggtg taccggttgc tcgcaccaaa
     3661 attattatct ttgttctctc tggcctggta tctgcgatag ccggaattat tctggcttca
     3721 cgtatgacca gtgggcagcc aatgacgtcg attggttatg agcttattgt tatctccgcc
     3781 tgcgttttag gtggcgtttc tctgaaaggt ggcatcggaa aaatctcata tgtggtggcg
     3841 ggtatcttaa ttttaggcac cgtggaaaac gccatgaacc tgcttaatat ttctcctttc
     3901 gcgcagtacg tggttcgcgg cttaatcctg ctggcagcgg tgatcttcga ccgttacaag
     3961 caaaaagcga aacgcactgt ctgatgcttt tttccacaac aatttaacgt tttttcccac
     4021 cacagccagc cgccacaacg gttggctgtt cttcgttgca aatggcgacc cccgtcacac
     4081 tgtctatact tacatgtctg taaagcgcgt tctgcgcaac acaataagaa aagagaagga
     4141 ggagaaccgg gtgacagaac cgttaaccga aacccctgaa ctatccgcga aatatgcctg
     4201 gttttttgat ctcgatggaa cgctggcgga aatcaaaccg catcccgatc aggtcgtcgt
     4261 acctgacaat attctgcaag gacttcagct actggcaacc gcaagtgatg gtgcattggc
     4321 attgatatca gggcgctcaa tggtggagct ggacgcactg gcaaaacctt atcgcttccc
     4381 gttagcgggc gtgcatgggg cggagcgccg tgacatcaat ggtaaaacgc atatcgttca
     4441 tctgccggat gcgattgcac gtgatattaa cgtgcaactg catacggtca tcgcccagta
     4501 tcccggtgcg gagctggaga cgaaagggat ggcttttgcg ctgcattatc gtcaggctcc
     4561 gcagcatgaa gacgcattaa tgacattagc gcaacgtatt actcagatct ggccacaaat
     4621 ggcgttacag cagggaaagt gtgttgtcga gatcaaaccg agaggtacca gtaaaggtga
     4681 ggcgattgct gcctttatgc aggaagctcc ctttatcggg cgaacgcccg tatttctggg
     4741 cgatgattta accgatgaat ctggcttcgc agtcgttaac cgactgggcg gaatgtcagt
     4801 aaaaattggc acaggcgcaa ctcaggcatc atggcgactg gcgggcgtac cggatgtctg
     4861 gagctggctt gaaatgataa ccaccgcatt acaacaaaaa agagaaaata acaggagtga
     4921 tgactatgag tcgtttagtc gtagtatcta accggattgc accaccagat gagcacgccg
     4981 ccagtgccgg tggccttgcc gttggcatac tgggggcact gaaagccgca ggcggactgt
     5041 ggtttggctg gagtggtgaa acagggaatg aggatcagcc gctaaaaaag gtgaaaaagg
     5101 gtaacattac gtgggcctct tttaacctca gcgaacagga ccttgacgaa tactacaacc
     5161 aattctccaa tgccgttctc tggcctgctt ttcattatcg cctcgatctg gtgcaatttc
     5221 agcgtcctgc ctgggacggc tatctacgcg taaatgcgtt gctggcagat aaattactgc
     5281 cgctgttgca agacgatgac attatctgga tccacgatta tcacctgttg ccatttgcgc
     5341 atgaattacg caaacgggga gtgaataatc gcattggttt ctttctgcat attcctttcc
     5401 cgacaccgga aatcttcaac gcgctgccga catatgacac cttgcttgaa cagctttgtg
     5461 attatgattt gctgggtttc cagacagaaa acgatcgtct ggcgttcctg gattgccttt
     5521 ctaacctgac ccgcgtcacg acacgtagcg caaaaagcca tacagcctgg ggcaaagcgt
     5581 ttcgaacaga agtttacccg attggcattg agccaaaaga aatagccaaa caggctgccg
     5641 ggccactgcc gccaaaactg gcgcaactta aagcggaact gaaaaacgta caaaatatct
     5701 tttctgtcga acggctggat tattccaaag gtttgccaga gcgttttctc gcctatgaag
     5761 cgttgctgga aaaatatccg cagcatcatg gtaaaattcg ttatacccag attgcaccaa
     5821 cgtcgcgtgg tgatgtgcaa gcctatcagg atattcgtca tcagctcgaa aatgaagccg
     5881 gacgaattaa tggtaaatac gggcaattag gctggacgcc gctttattat ttgaatcagc
     5941 attttgaccg taaattactg atgaaaatat tccgctactc tgacgtgggc ttagtgacgc
     6001 cactgcgtga cgggatgaac ctggtagcaa aagagtatgt tgccgctcag gacccagcca
     6061 acccgggcgt tcttgttctt tcgcaatttg cgggagcggc aaacgagtta acgtcggcgt
     6121 taattgttaa tccctacgat cgtgacgaag ttgcagctgc gctggatcgt gcattgacta
     6181 tgtcgctggc ggaacgtatt tcccgtcatg cagaaatgct ggacgttatc gtgaaaaacg
     6241 atattaacca ctggcaggag tgcttcatta gcgacctaaa gcagatagtt ccgcgaagcg
     6301 cggaaagcca gcagcgcgat aaagttgcta cctttcctaa gcttgcgtag gagcggttaa
     6361 tctcccgtaa gtggaactaa cagcacatcg acctcacttg aggcaatcac tcttttcgcc
     6421 gagcaggatg ctcgcgaaaa gaaactgtga ttgtgattac cgcaaatcac caaatcgaaa
     6481 tgatatttgc gacaaacttc cagaatatgt tcgcttaact caccgtaggc aataaacgtc
     6541 ttgtcaacgg gataccccgc atcctgaatt aacttatcaa gaaagctttg cgtttcttcc
     6601 tgcatcacgc tgcgtaaatc ttcaagcatc ggcgcagcta attgattgta catttccggg
     6661 tcggaagcga gagtaattaa actgatgtgt ccctttactg gcctggcgat agataccgct
     6721 tttgccagca gttgctgact ttccggtgta accgcaacag cgacaagaat attgctatag
     6781 ctcataacct gttccttatt ctgtgaactt caggtgacat taaagcttta ctcctaatgg
     6841 agaaacgacg caatcccaac tcagtcaaac aggtaaatat tagtctgatt attagtaaag
     6901 acaattaatc aatcactctc ggtgatcata tgatttcaac tgtaattata agaaaataat
     6961 gtactgattc cccgcattaa acaagtggtt aatatatgca aaacatttta ttaacattaa
     7021 gttgattgtt gcctttcttt gtatttaatt agtttgttgt gcggtaagtg tctgtt
//
LOCUS       DAZKFZ010000229         6940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000229 DAZKFZ010000000
VERSION     DAZKFZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2775)
                     /gene="pqqL"
                     /locus_tag="ACHOQW_004163"
     CDS             complement(<1..2775)
                     /gene="pqqL"
                     /locus_tag="ACHOQW_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9575127.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLL"
     gene            complement(2832..5204)
                     /gene="yddB"
                     /locus_tag="ACHOQW_004164"
     CDS             complement(2832..5204)
                     /gene="yddB"
                     /locus_tag="ACHOQW_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9575128.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5242..6927)
                     /gene="yddA"
                     /locus_tag="ACHOQW_004165"
     CDS             complement(5242..6927)
                     /gene="yddA"
                     /locus_tag="ACHOQW_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9575129.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1790 a         1594 c         1566 g         1990 t
ORIGIN      
        1 caataatact ccggtataag tatttaccgg atgagaaaga tattgtttaa cggcagtgtt
       61 aacattctct accgtcattt gtttcaacaa ttgctcctgc tcagtccatg ctgcaggatc
      121 gtcatattga ataagactat ttacaatagt gttcgctaat tgttgaacgc tacgctgttg
      181 gatatcgagg ctgcgctgaa cgttttgctg gtattcgttc agttcttgct cactgatccc
      241 tttagtcaaa cgcttaacca tcacttcatt cgctaacgtt aacagttcat catgtcgttc
      301 tggttgacaa gtaaaagcca gcaaatgact gatatcttta gcctgaggat caaccgagag
      361 gcgagaagaa acgctgtatg ccccagatgc ctgttcacga atattaacgc gtagatcttt
      421 tgccagtgcg acgttaaaag catcgagcgc catacgcgtc gccagattaa caggtgtccg
      481 ggaatcataa cgcttccact gtgaaacctg tgccacaggt tcattttgtt cctttacagt
      541 aaccgatgcg ttgtccgtcg cgcgagttaa tggtttacct gcggctaatg gcgaatcaga
      601 gtgtttgatt gatcctaagt aacgcgtaat taacgccacg agtttgtctt ctgcgacatt
      661 accgacaatg acaaacgtga tatccgctgg agatgaaaac aactggcgat cggcagccag
      721 cgcatctgcg gcagtaaact gtgcaatctg attttcttgc agtaatttcg tgcggtcatc
      781 agcatagcgc gtctcataca tctgctgggc gaatttctcc gctggacgct ggtcgagcgt
      841 tttcaatgcc tgaatttgag cattttgtag cgatgcccaa atattgtcgt taatcgtgct
      901 gtgggtgatt cgctggttaa tcaactgaaa accaggttca gggttattag tccgcgcgct
      961 aacggagagc aacgtattca tgccactgac tttactgctc atggttaccg agttttccgc
     1021 actccagcgt ttcaggctgg aagaggagag ttcgccaacg ccgcttccgc taactgcttt
     1081 atttgccagc gcgataagtg atttttgctg cgcagggaaa cttaaatcgc ctttattcga
     1141 tacggcaata atttgtagct tttgctcttc acccgcggat tttgccagaa taacccttgc
     1201 accattggaa agtgttaatg atgtcagatt ctcagccagc gtttctttgc tgctaatttc
     1261 cgcctgtggg tcagcgtcta ctgttaacga taaatttctg cctgggaaga tgtaagccgc
     1321 cagctttttg ttggcgtact ccttttccag cgccaggata gccgcaggag acaatgcttt
     1381 tttggcggca acctcattgt ttaccatttg ctcccaaaat gcgtcctggt tctttcttaa
     1441 ctgctgccat ttttccgcca gactttgcac ggtaatttgc tgccacagac gtttcgaaag
     1501 ttgatatgtc tcttccggcg acaagaacgg cgtattattt aatgagctgg atgccaggcg
     1561 actggtcagc atacgtaaat cacgttcagc ttgctgatca accgcatttt tcagccaggt
     1621 gaggcgggta gatttgacat catcgagttc ttcagcagaa aaaccatgct gatcaatggt
     1681 tgccaactct tccattaatg cattcgcagc atcctgcata ttatcgtctc gtgcatttac
     1741 acggaaaaac agcgactgat aatcgggtgc aattttaacg ctgcgcgcag tgccgccaga
     1801 aatagtcttc aactcgcccg actgtatacg ttcctgcaga cgttgattga acagctgaac
     1861 taacatgctc cattcagctt gttcgataaa gctttgctca tcgttcactt gtaccattgg
     1921 caggcgataa tagagtgcga tgccgttcac ccggttttct ttatcattaa tgatattaaa
     1981 gcgcaggtgg ttttcggctt ttgtcggcca gacgcgattt tcagctgctt tgttagccgg
     2041 aagcttactt aaattatcct ttatcagcgc cagcgcttct ttactgtcga tatcgccaac
     2101 cacgataaag gtcatattat ttggttgata ccagcgttga taaaattgga gcaattgtgc
     2161 cggtgtgacc gtggcgacag tatccatcag gccgataggt tcacggtcta aattacgggt
     2221 atttgccagc aggaaagggc ggcgcgcctg agaggtgcgc catttcgcat cctgatgggc
     2281 acgccattcc tcagtaatta cgccgcgttc agcgtctact tcgagttttt caaaggttgc
     2341 ggcattactc cattcactga agattgccat cacttgttgc agattttgtt tctgcgtagt
     2401 tggcaaactc acctgataca ccgtttcgtc atagctggta taggcattaa catcgcgacc
     2461 aaaacgcagg cccattgact caaatgtttc gatgacttta ttacccggcc atgtttttgt
     2521 gccgttaaac atcatatgtt ctacaaaatg agccacgccg cgctcattgt cttcttcctg
     2581 caatgaaccg gtatgaattt gcagccataa atttacctga tcctttggat gagcatgcgg
     2641 ataaatcata tatcgcaagc cattgtccag ttgcccggta attaactttt catcctgcgg
     2701 taaggcggcg gcaatcagcc gcccggggag caacagagtt gccactaacg tcagtaagaa
     2761 acagaggttt ctcataatta tctccatgcg aaaaccgggc gaatttaccc ggttaagtaa
     2821 aatccgaact attaaaattt catgctgaca tcaagccaga aagtacggcc actggcgtag
     2881 gtcgccacac cggtattcgt tgtatcaacc gcggttttgc tatccagtac attgagaata
     2941 tcggcactga tcgtcaggtt ttgttgtttc agaaattgtg gcgtccagga caagcgggtg
     3001 tcccaggtca ggctgctatc cagtttttcg tcaacatact gcttgtaatc tgaatattcg
     3061 ctgatgtatt gcgcatttgt cttacccagg ataatgcgag ctttacgcgc ttcttgccag
     3121 gccagcgtat tcgcccacac caacccgctc ggttgatgcg tgaaatccat gtttaaggag
     3181 atctttaatg ggttattaaa atctgccact ggaacgctat cgtaagagac aagattaccg
     3241 ttataaacca cctggttatc accggtattg ctctcctcat aaccattgtt taacgacaag
     3301 ttgcccttgc tcttgatata gctaaagaca atttgcgggt taatatctac ctggctgata
     3361 tgcaggggtt cggccagttc aaaactgagg ttgaacgaat gcgttttggt tttgccgtcg
     3421 ttgttatatt cagtaatggt ggttttagtc gcgctgtcgg tacgactgct tttgctgatt
     3481 tgatcatgcg cttcacggta aacatagttt gcgcgcgcaa taacgttctt accgattttt
     3541 tgctgcaatc ccattgccag ttcatcgtta taaggcgttt tcaaatcctg ataacgcgtc
     3601 agggttttat tacctgatac cgattccgtc cagctattgc ggatatcacg taatcccata
     3661 tcaagaatat tcccgccata gtaacggtta taacctgctg taatcattga ggtttgatca
     3721 gcaaaaatat cccattccgt cataaagcgc ggggagatat tgtggtttga cagatagttg
     3781 tcatagtcat accgcacgcc gggcattaat gacacattac gccagctaat gcgatccgcc
     3841 atatacagtg tgtagttgtc aattcccagg ctgcctttac ctttatggta aatggtatgg
     3901 ttagttttct ttccggcagc attaatcaca taggattcag actggttatg gcgttcagtc
     3961 cacgcatcgg aatagatgta ttccgcgccg aagtagggtt gatgcgaaac attacccacg
     4021 gcgaattttt gccagtccag gcgtgttttg aaggtgtaat tatctacagc ctgggaaatg
     4081 tgtcctaatc cgccacgggt gcaacgccct gtaatatcac catatgtaca tgaaagttcg
     4141 gtgtaccaga tgtcatgatc gtgacgggta taatcactaa tatgatccca accaacggtg
     4201 gtacgtagtt tggcccatgc gagctgcgta tccatatccc atgccatacc ataggattta
     4261 ttacccattt cgcgatcaga ctgcgggaag gtgctggtat tataatcacg gctggagccg
     4321 gtatatttta aggttaaatc gtgggtaaag cggtcgctgg caaaccaggt aaatttgctc
     4381 aatgcagtat cgataacgtt tttatactgt gcccgaccgg cgacaatgcc gtcattcgaa
     4441 acataatccg cgcgggtgat atcagactgg cggcgcgata aaccggcggt aacgccaaag
     4501 ttatcagcga gttcctgatt aaacgacaag gtataaaagt tctttttaaa atctggggag
     4561 taataagtac ttcctgaaga gccttgatta aatgcgctct tgttattctc attgatatgc
     4621 gatgttaacc agtccgaacg cgtagtgcga taacccagtt tcacgctgct atcatcagcg
     4681 ttgaagcgtt tgaactttgc atcaattacc ccgccattga agcgaccgaa ttcaaccggc
     4741 acaaaactgt cataaagcgt cacattgtcc agtaagctga catcaagata atacccctgt
     4801 gacatcccgc taatattggt tgcactactg gcatcggact cattcgctgg gttcaggtta
     4861 ttagtggcac taataccgtc aatcaaatag gcattctgat agggcgacgc accgtgaata
     4921 gagattttct ccgggcgaat atctccctgg ttcaacgagg tactttgcgt tgaatccatg
     4981 cgtaccgcag ggttggttct cagcagatcg ctgatattgc cattcccggt cggtaaacgc
     5041 tcgatgcttt cactggtgta atgcgtgttg ccgtttacgg gtacagggac aggagcatag
     5101 accgtcatct cttcaaaaaa atgcatgtac atgtttttgt catcgacggc ctgcgccaca
     5161 tcagcgccac ataaaatgac gccaggaata agaactcgct tcatttattt atatctccgt
     5221 acagcagggc ttttattgtt tttataaaac cgcgctaatg tcacaaatat catcggccag
     5281 gttccagaca ccgggttgat gtgtaaccat aatgacgccg cttgtgggta gtttttcacg
     5341 cactagacgc agtaagcgga tagcctcttg ttcctcaaga tgagaggtag tttcgtcaag
     5401 aaatatccat ttcggacgtc gtaaaattaa tcgtgccagg gcgatacgtt gtttttcgcc
     5461 gctggaaaga atatctcccc agcgatcgtg gtcatgaata cgcgcagcca atttcccaag
     5521 accaacctga tgcagtactt cgctcaatga tttatcgtct acgggcaggg gaagtgcttt
     5581 acaaataatc tctttcagta agccggtttt gattaacggt gtttgtgaca cataccaact
     5641 gtcggcagga gaagaaatat cacctttaaa ccacggccag cagtgggata atgttttaag
     5701 cagtgtggtt tttcccgcac cagagtaacc tttcagtaat agccatttgc ctggcgaaac
     5761 atgaaagttc aggttctcta atatgatctt attatcagga gtacgaatac tcgcgtcagc
     5821 cacttgtacc gcatgttggc aatttttagg cttattcgta gggcgctgtt cggtgagttg
     5881 atggaactca tacaagcgat cgataaccgc agccagttca gcaagttcat catatttata
     5941 aataaaccag cttagattgt tcgataccag cataaatgcc tggcgcgatt tcatcagtcc
     6001 gcccagattg atctgcccgc taataaactg cggcaataac agaaagtagg gaagaacgct
     6061 aagcgagcgc gaataaatat tctgccagta atcaagccac cgctgacgat tcattaaacg
     6121 atgccagttc tctttaatgg tatgaaaatt gtcgctcaac tcctggcgtt gtagagactc
     6181 agcattgctt agcgcaatca gttctgcctg cttattatgc tgcacaagat tagttcgaaa
     6241 tgtcgcttcg ctacgttgtt tttccacatt aagcggacga atacgtttac ccaccttatg
     6301 ggtaaataaa gttccaccga tcacaatgag cacaacggta tagaccatat acccctggat
     6361 attccattct gttccaccaa cagtgaatga gagcataccc gcgctttgcc aaagaataac
     6421 ggtaaaggtg atcagcatgc taagtgactg gatgaagcca aatgaaagac tgagcgtttt
     6481 gctgatcagt aagagaatgt cttcagcgat acgttggtca gggttatctg tatttttatg
     6541 ctcgccgtag atctgcgtga agtagtagtt tttatctgca aaccagcgat taaggtagta
     6601 atctgttaac cattcgcgcc agcgaatagt cagtagttta attaaccatg ttttgtttac
     6661 ggatattaat acaaagatcc ccagcagggc agggaaccaa aggacaagct gccaaagctt
     6721 atcggtttct ttctggctta gcgcattgaa aaaatcatta ttccagtcgt ttaaccagac
     6781 ctgaatttta accacgccaa ggatcatggc gaggataata atgattaaca acactgaagt
     6841 tttattattc ttacgcagcc aaaagggctt cagcagacag agatactttg ctatcaacat
     6901 acaaaacgta atgggaatgg ttatcattag cgaaaattga
//
LOCUS       DAZKFZ010000230         6917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000230 DAZKFZ010000000
VERSION     DAZKFZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(173..658)
                     /gene="sixA"
                     /locus_tag="ACHOQW_004166"
     CDS             complement(173..658)
                     /gene="sixA"
                     /locus_tag="ACHOQW_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP9575130.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(861..3005)
                     /gene="fadJ"
                     /locus_tag="ACHOQW_004167"
     CDS             complement(861..3005)
                     /gene="fadJ"
                     /locus_tag="ACHOQW_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP9575131.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3005..4315)
                     /gene="fadI"
                     /locus_tag="ACHOQW_004168"
     CDS             complement(3005..4315)
                     /gene="fadI"
                     /locus_tag="ACHOQW_004168"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP9575132.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4495..4779)
                     /gene="yfcZ"
                     /locus_tag="ACHOQW_004169"
     CDS             complement(4495..4779)
                     /gene="yfcZ"
                     /locus_tag="ACHOQW_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP9575133.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5151..6491
                     /gene="fadL"
                     /locus_tag="ACHOQW_004170"
     CDS             5151..6491
                     /gene="fadL"
                     /locus_tag="ACHOQW_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP9575134.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6856..>6917
                     /locus_tag="ACHOQW_004171"
     CDS             6856..>6917
                     /locus_tag="ACHOQW_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575135.1"
                     /translation="MLSSISINNTSAAYPESINE"
BASE COUNT         1745 a         1859 c         1620 g         1693 t
ORIGIN      
        1 cattacaact tagtctcatt tcgtcagaaa ttaataggga agcagaggaa aaagtgcgat
       61 gaattctgta tcgaagcgta tcaactccgt gaagggtttt gagtgaattt ctactattgc
      121 aaaaagacaa atgcccgatg tacagcttac atcgggcatt ttttcaaacg aatcagatag
      181 ctttcgccat cttcaaatta catgggctca tctgccagtt gaactcgcct tttccgcttt
      241 catcaagcgt tacgctggca atggccgagg tggtaaacat aggcggcgtt tcgcccggac
      301 ataactcggc gaccagatac ccaactaacg gtaagtggga gataaccaac accgaagcaa
      361 caccttcatt ggtcaacgcc tgcaaatagg cactgaccag accaacatcg ccgcagggcg
      421 ttaactccgg cagaacttct gcactggaag gcaggttcag acaatcccca acttcctcca
      481 gtgtttgctc ggctcgcagg aacgggctca ccagaacgcg ttcgatttcc actttttgac
      541 ctttcagcca gttcgccatc aggcgagatt cgtcacaacc gttagtggtc agaggacgaa
      601 ccgaatcact ggcggcatcg agggctgcgt cgccgtgacg catgataaaa acttgcatat
      661 tgcaccgctt ttgttaacca gattcgcccg ttttctttca tctgaaacca gagaaagaaa
      721 acggtggccg gcattgtgcc ttatccattc accgaatgaa acgctgtttt ttacctcaat
      781 ggcgtaagta tagtcaatat gcgtttacat tttgaccaac aatccgccat tcagcgcgga
      841 ttcatatagc tttgaccttc ttattgcagg tcagttgcag ttgttttcca aaaactttcc
      901 ccacgcgcac ccatctcgac taaacgctcg caaggggtaa aacgtgaacc atactgcgtg
      961 gccagtcgtt gcattattgc aaccacttcg cccgcgccga gagaatcgat atagcgaaac
     1021 ggtccaccga gaaatggcgg aaaaccaatg ccaaataccg caccaatatc cccgtcacgc
     1081 acgctacgga taacctgctc atcaacacaa cgtacagctt cattcagcat caacatcaca
     1141 caccgttcag caacctgcgg tgcggagagt cgcccctgcc cttgtgcgcc aatgagcggg
     1201 taaatggcag gatcgacctg ttttttgctt ttacgccctt tctgaccata aagatagaaa
     1261 cctcgaccat tttttctgcc tttgcgatcg tcgttcaaaa ttgaagaaac aacatttgca
     1321 ggcgcgctaa aacgttctcc ataagcggct tccagtacag gaataatttt agtcccggtg
     1381 tcgattccta cctcatccaa aagttggatt ggacctaccg gaaaaccaaa tttcactagc
     1441 gcggcatcaa tgtgcgctac ccgttcacct tcggtcagca tgcggatagc ttcattaatg
     1501 taaggcgcta agatgcgatt gacgtaaaac ccggctttgt cacgcacgac aatgggcgtt
     1561 ttgccctgtt ttttcgccag ttttaccgta gtggcgatgg tttgtgctga tgtacccgca
     1621 tgaggaataa tctccaccag cggcattttt tccaccggac tgaagaaatg caggccgata
     1681 acttgctcag gtcgcgtggc gtgagcggcg atatcaccaa tcggtaaaga tgacgtattc
     1741 gaagcaaaga tggtatgagc ggcgcaattt tgctcaactt ccgccaccat ctgttgtttc
     1801 aattcgagat tttcaaacac cgcttcaata atcagatcgc gatgggcaaa gccgcgatag
     1861 tccgtcgttc cggagattaa tgccagctgt ttgtcacgtt cgctggcttt gagatgacga
     1921 cggcgaactt tgccctccag ctgatcccaa ctgtacttca gcgcatgatt tatgccctgc
     1981 gggttgatat ctttaattct gaccggaatc cccgctttac aagcagtgac ataagcaata
     2041 ccgccgccca tcaagccacc acctaaaatc cccacgctgt ttaatggcgc aggcggcgca
     2101 tcactgccgg gatctttctt cacttccgta ctggcaaaaa agatactacg caaagcttgc
     2161 gattgtggcg tcatcgccag ttcgccaaac gcccgtgctt cagcgtcata gccactgctg
     2221 gtgccctgcg ctaatcccgt ttcaacaacc tccaggatgc gttctgtcgc cggataattg
     2281 ccttgagttt tgtgttctgt tttcttgccg accattttga acagcagcgc acgacctaac
     2341 ggccccgcca gaatacgctc gcgtacaggt agagggcggg aagatgggcg atccttcttt
     2401 gccagctcaa cagcggcttc cagcagaatg gagtgcggaa caacatcatc caccagcccc
     2461 agctttaatg cctgtttcgc ccgaagttgt tttccggtga ggatcatctc taatgctgtg
     2521 ctgacgccta tcagacgcgg taaacgctgg gtgccgcctg aaccgggtaa caatccaagt
     2581 tgtacttcag gcaaaccgag caccgtttta ggatcgtcag tacaaacgcg accgtggcac
     2641 gccagcgcca actccagccc accacccagg caagcgccat gaatagccgc gataaccgga
     2701 atgggcaaag catgaatctc cgccatcaac tgttggccct gccgcgccag agcttccgct
     2761 tcttgcgccg ttttgcaatt gccgatcatg ttgatgtctg cgccagcaat gaagttgtcc
     2821 ggtttagcgg agacaaacac cacgcctcgc aactctttgt tttcacggag ttgcttaata
     2881 atggcgcgca cctgcgaggc aaactccgcc ttcagggtat tcattttctc acccggtacg
     2941 tcgatggtga tgatagcaat gttgtccaga cgaacattaa gagtaaacgc tgatgtcatt
     3001 tccattattc cgcctccaga accattgccg cgccaagccc accggcagca caggcggtaa
     3061 ctaaaccaaa tccaccgccg cgacggcgaa gttcatgcaa tgtctgggta atcatccgcg
     3121 cgccggtcgc cgcgaaggga tgcccgtaag caatcgaacc gccaagcacg ttaaatttgc
     3181 tatcgtccac ttcgccagtg gcatgtgcac gccccagcgc ttcacgagca aaacgttcac
     3241 tacccagtag ctgaatattg gccaacgtct gggcggcaaa ggcttcgtgc atatcaatca
     3301 atgtcagatc tgccattgtc aaaccggcac gctccagcgc cagcggtgtt gaccaggctg
     3361 gaccgagcaa catgtcctgc cagacatcga tagcggtaaa tgcgtagctg cgcagatatc
     3421 ccagcggtac cagccctaat tctttcgccc gggattcagt catcaggatc accgccgctg
     3481 cgccatcggt cagcggcgta ctgttcgccg ccgttaccgt tccgtgttta cgatcaaacg
     3541 ccggacgcag ctttgcgaaa tcagcaagcg aggaattacc gcgaatattg ttgtcttccg
     3601 cgagcagttg tttataagga gggataaagg cagtcatcac ctcttctttg agttttccgt
     3661 ctgaccatgc ctgagcggca cgctgatgcg aacggtgcgc taatgcatct tgctgttctc
     3721 gggtgatgcc gtaggttttc gccatttgct ctgcagtgtc gcccatccgc aagccggtag
     3781 aatattctgc taccgcaggt ggtacgggca ttaagtcgcg caaacgcagg cgagagaaga
     3841 gtttcagacg ctggctcatt gtgcgggctt tgttgacatc aaccagcacg cgcgccagtt
     3901 ttttactgac gccaattggc aataccgaag aggaatctgc cccaccggca atccccgctc
     3961 ggatagtccc cgccatcagg ctttctacga cgtttgcaac cgcctggaaa ctggtagcgc
     4021 aagcgcggct gacgctgtaa gcatcggtat gcacattcat tccagtaccg agaacaattt
     4081 cacgcgcaat gttgggagct tcaggcattt gtacgacctg accaaagacc agttgttcaa
     4141 tcacttcggc ggggatctcg ctgcgtgcca gcagttcgcc taccaccatc ttccctaaat
     4201 caaccgcggg aatgccatga aaagccgtcg cctggcgggc aaaaggcgta cgtaaaccgc
     4261 taacaatggc gatacgatcg ccctggcggg taaccagcgg taaaacctgc cccataacac
     4321 tcccctgtaa aaaaataaat aaagtggtct gacctgatca tagtcttaac cattttttta
     4381 catttagcca agcggagaaa agggaaattg ggagctatga cacagtgaag acaggagaaa
     4441 gaaaagaaaa cgcccctacc tgttgctggc aggagcgtac ttatccgaga ggaattaacg
     4501 cagacccagc tggaagatca gcatttcagc ttcgcaggca aaagtgaaat cgatatccag
     4561 gcgcacacca tcagactctt cagtgaaagt cggggagatt ttgcacggtt cggattccac
     4621 gctacgggct ttctcagtca gcgccgccag cgtttgttct gcttctgcgc ggtttgcaaa
     4681 cacgcggctg taagacgctg tgcagtcgga gttgtccata atggtgccaa catccataca
     4741 gcagcaaacc ggggtttcat cagcactaca tttactcatc gttgatttcc tctgtatgtg
     4801 cacccaaggt gccagataaa cgttgtggat attttacgct tccggaaagt gctgctccag
     4861 ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca cttaaaaatg atctaaaaca
     4921 aaattcaccc gaatccatga gtgcgccacc tccaaatttt gccagctgga tcgcgtttct
     4981 tagatcatat ttgaaaaaag atagaaacat acttgcaaca ttccagctgg tccgacctat
     5041 actctcgcca ctggtctgat ttctaagatg tacctcagac cctacacttc gcgctcctgt
     5101 tacagcacgt aacatagttt gtataaaaat aaatcattga ggttatggtc atgagccaga
     5161 aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc acttatctcc acccaggcct
     5221 ggtcggcagg ctttcagtta aacgaatttt cttcctctgg cctgggccgg gcttattcag
     5281 gggaaggcgc aattgccgat gatgcaggta acgtcagccg taaccccgca ttgattacca
     5341 tgtttgaccg cccgacattt tctgcgggtg cggtttatat tgacccggat gtaaatatca
     5401 gcggaacgtc tccatctggt cgtagcctga aagccgataa catcgcgcct acggcatggg
     5461 ttccgaacat gcactttgtt gcaccgatta acgaccaatt tggttggggc gcttctatta
     5521 cctctaacta tggcctggca acagagttta acgatactta tgcaggcggc tctgtcgggg
     5581 gtacaaccga ccttgaaacc atgaacctga acttaagcgg tgcgtatcgc ttaaataatg
     5641 catggagctt tggtcttggt ttcaacgccg tctacgctcg cgcgaaaatt gaacgtttcg
     5701 caggcgatct ggggcagctg gttgctggtc agattatgca atctcctgcc gggaagactc
     5761 ctcaagggca agcattggca gctaccgcca acggtatcga cagtaatacc aaaatcgctc
     5821 atctgaacgg caaccagtgg ggctttggat ggaacgccgg tatcctgtat gaactggata
     5881 aaaataaccg ctatgcactg acctaccgtt ctgaagtgaa aattgacttc aaaggtaact
     5941 acagcagcga tcttaatcgt gtgtttaata actacggttt gccaattcct accgccacag
     6001 gtggcgcaac gcaatcgggt tatctgacgc tgaacctgcc tgaaatgtgg gaagtgtcgg
     6061 gttataaccg tgttgatccg cagtgggcga ttcactatag cctggcttac accagctgga
     6121 gtcagttcca gcagctgaaa gcgacctcaa ccagtggcga cacgctgttc cagaaacatg
     6181 aaggctttaa agatgcttac cgcatcgcgt tgggtaccac ttattactac gatgataact
     6241 ggaccttccg taccggtatc gcctttgatg acagcccagt tccggcacag aatcgttcta
     6301 tctccattcc ggaccaggac cgtttctggc tgagtgcagg tacgacttac gcgtttaata
     6361 aagatgcttc agtcgacgtt ggtgtttctt atatgcacgg tcagagcgtg aaaattaacg
     6421 aaggcccata ccagttcgag tctgaaggta aagcctggct gttcggtact aactttaact
     6481 acgcgttctg ataacgcgtt cgcctggata aagtcacctg ctatgcaggt gactttaact
     6541 cccccacttc accgaaagta gtttcctcat tgcttgcctc ctgagttttg caaacagcct
     6601 gatggcagct agccctttca aatcagtaag tggtatccac agacaaacct gaagggaaac
     6661 gcatattttc aggcgttctg ctcgtccttc tcaaagagtt tacttttctg aatttccagg
     6721 atactcaacc ccctggctat tgtgcgctca tacacgcaaa ttaaagatag gttctaaata
     6781 aatgagcgtt ttttgatagt ctatttcatt aggtaatata tatttgtaac aaatcaatca
     6841 acatggaata aaatcatgct atcatctatt tcaatcaaca ataccagcgc agcttaccca
     6901 gaatccatca atgaaaa
//
LOCUS       DAZKFZ010000231         6914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000231 DAZKFZ010000000
VERSION     DAZKFZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(47..1423)
                     /locus_tag="ACHOQW_004172"
     CDS             complement(47..1423)
                     /locus_tag="ACHOQW_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9575136.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            complement(1420..2241)
                     /locus_tag="ACHOQW_004173"
     CDS             complement(1420..2241)
                     /locus_tag="ACHOQW_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9575137.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEITDSLLMADLT
                     VRQLKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAKSANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GDGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2228..2389)
                     /locus_tag="ACHOQW_004174"
     CDS             complement(2228..2389)
                     /locus_tag="ACHOQW_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575138.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(2422..2718)
                     /locus_tag="ACHOQW_004175"
     CDS             complement(2422..2718)
                     /locus_tag="ACHOQW_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9575139.1"
                     /translation="MERTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(2860..3075)
                     /locus_tag="ACHOQW_004176"
     CDS             complement(2860..3075)
                     /locus_tag="ACHOQW_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9575140.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            3150..3845
                     /locus_tag="ACHOQW_004177"
     CDS             3150..3845
                     /locus_tag="ACHOQW_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9575141.1"
                     /translation="MDGSSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLSDIGVVFKYLGIDNISFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERLVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     gene            4347..4868
                     /locus_tag="ACHOQW_004178"
     CDS             4347..4868
                     /locus_tag="ACHOQW_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575142.1"
                     /translation="MAKSNVSVQAFKDFLEELMSLNIMKEATARNLKNSSARLLTVVQ
                     EEEMGDVTQLDVNELAERYINATEPKPSDSSITAYKSRMESAIKKFVAFQSGEEIPYT
                     PIDKESSEEKDLTGEPTKVEGKANALHTYDLPVVLRPESGVTVTIKGIPNDITNEEAE
                     RISSILKVYVRPQ"
     gene            5437..5619
                     /locus_tag="ACHOQW_004179"
     CDS             5437..5619
                     /locus_tag="ACHOQW_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575143.1"
                     /translation="MEQTGRLFKQRRLSTTWLKSQITQPHKLWDAMPKQPSQEELRDC
                     IAKVYSGGIYVQKNRI"
     gene            5597..5869
                     /locus_tag="ACHOQW_004180"
     CDS             5597..5869
                     /locus_tag="ACHOQW_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP9575144.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVALYAAGYRKSKQLTAR"
     gene            5928..6179
                     /locus_tag="ACHOQW_004181"
     CDS             5928..6179
                     /locus_tag="ACHOQW_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575145.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            6362..6730
                     /locus_tag="ACHOQW_004182"
     CDS             6362..6730
                     /locus_tag="ACHOQW_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFP9575146.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            6803..>6914
                     /locus_tag="ACHOQW_004183"
     CDS             6803..>6914
                     /locus_tag="ACHOQW_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP9575147.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDIL"
BASE COUNT         1887 a         1629 c         1559 g         1839 t
ORIGIN      
        1 accattagta acgataaccc tgctattagc tcagtgatgt agatggtcat acgtcagccc
       61 cttgtgcata tcgtctgcca cgcgcagcag gtgcatttga tgctgtgcaa atctgtctgg
      121 cttcatcctg gtcacatgca acaaagtgtc cgttacagaa ccgctggtaa actgtaccaa
      181 gcgagccaaa acggtttttc gtcacgatga tttcagcaaa tggcgcggcg ctactgttct
      241 catcatatac cgcttcccga tagagcatga tgattgagtc tgcgtcctgt tcaatgcttc
      301 ctgaatcacg caaatctgcg tttgtcgggc gtttgtttgg tcgcttctca acatcgcgcg
      361 aaagctgact cagggagata accggtgttt tcaggtcttt cgccatcgcc ttcaggcttc
      421 cggagatgtg agcaattgcg aggtcgttgc ggtctgcttt cggcttctca atcaggccaa
      481 gataatccgc catgatgagt gacaggtttg gattttcctg cttatgccgt tctgcgattg
      541 agcgtatttc ttcgaccgat aaccgcgaag catcgactac ccatacatcc aaatctgcaa
      601 gctgactcat gccgttagca acacgtgccc agccctcgtc atccatcgat gcaggatttc
      661 gcagtacgct aaccgacatc ctcccggcgt tggcaatgct tcgctctgca atctgcaatg
      721 cgctcatttc cattgagaaa atcaataccc cgcgccggac gtcagaacca ggaataacgc
      781 ggcttgccac accttcggca atcttcagcg ccagttcggt tttccccata ccaggacgag
      841 cggcgataat caccaggtct tctgcgttca tccctccagt gatagcgtca agctcttcga
      901 ttccggtctt cagggtatct gactcttctc cgttcctcag acgcctgtca agcgtgtcag
      961 tgtagtcagt gatgatttcc cctaaccgta caggtttaac ctcgtcacgg ggctttctga
     1021 tggctgaaag acgctttaca agttcatcca tcgcctgact cgatgcgtcg atggttccgc
     1081 tttggattgg ttcacgcatt tcatccatga tttccagcac cagacggcgg tgatagttat
     1141 ccgcgaccat tccggcatat cccttcaggt ttgcggcact cgggcagttt ttgctggtca
     1201 tcaggattga cgtgaaatgc tcctctccgc acgcctcggc aaccatcagc gcatcgatta
     1261 ggtttctgtt tctcgcctgc ttgcgaataa cctcgaaggc tttccggtag agcggaattg
     1321 aaaacgcttc cggctccagc gttgccagaa cgtcactggc ggttggagtt aatccaccaa
     1381 tcagcaggcc accgataacg ctagcttcga tatcctgtct catgcaatcc ccctgtctgc
     1441 aaacttccct tcccgaactc ccgttaacga atcttccctc agcaggtaat caaaatctgc
     1501 cgtccagccc gtgtcgttgt ctccgaagta aaacggcttg gcctgatgca caaacgccct
     1561 gacatacgct ctgaaaccgt ccacgtttgg cgttttcagt tgcgggataa ttttcttcag
     1621 gcggcgtttt cgtttctcgt tgaccgcaac agcgtgtgga agtctgtcac cgacttcggt
     1681 gttgtaggcg ttcaggaagg attcgtagtc gattcgttct gccttgcgac gttcaggttt
     1741 aacctgccca tcgccatccc cgttaggggg taagggggta tttgtattta ttgtcttttg
     1801 tatattgtct tttgtgttta gctgacttgg cttataccca ttagccgact tggctaatgt
     1861 tttattagct gttttagcta atgttaagct gtcctggcta atccactgag aaaccacctt
     1921 gttcactccg attttcacgc catcagcaat gaggaattta cgctcaataa gctggcgctt
     1981 ggcagcgcaa acatgagtgt gatgaatacc tgtcatggct gctatctgcg tgtttgtgag
     2041 tcgatccatc ggcttattga atccgtatgt cttgcgcatg atagcgagca tcaccttcaa
     2101 ctgccggacg gttaaatcag ccatcagcag actgtcggta atctcgttag caacgcgcat
     2161 gaaaccatct tcggtatctg ccacgcgatg ctccacgacc tccagttgag gcctgtaatc
     2221 agctaactta acgacgccca tgtttcactc ctgctttggc tagtctgtaa acaccaacaa
     2281 ggcgctctgc gaacgccctg ttatttgctg cggctaccac taatccctca ggtgaatcag
     2341 ggtgtcgaat ctcttctttt tcctggtatt tcttacgacg ttttgtcata attactcctg
     2401 tggattgatc cagtaattcc ctcagaattg catatcaatt tgcttaaaat cctcggtggc
     2461 agccggggat tttttctttg tgatttcatc aagcgcatac ttaaaagccc tgctaatcgg
     2521 actgatgtct gatgccattc cgaaagcaca caagaccgaa gcaataaacc gccagtccgt
     2581 tctgcttatc ttcgattcat gacagccaat catctttgcc agaccgcgct gtgtaagcgt
     2641 tgacaggttg atgagtaaat ctgtttctgc gcgatcaacg tcgcgctgtg atagtttgct
     2701 gtaacttgtt cgttccattt cttaagattt ccaatagtga atagttagtt gaaaggtatg
     2761 cgtggaaacg catatggcct tagttggtca gatatcttgg gactcgcttt tcagcgacgt
     2821 aggacgaatg tccgttgtta caaagagcgg ctccgcttat taagcggctt tgtgctccgg
     2881 cgggaacacg tcatcaagac ttacttttgc gcctaacttg tttagacacg caacaagagc
     2941 acggcatgtt ttaaggtctg ggaagcgacg accagattcc caatgtccga tagctccctg
     3001 tgtgcatcca actgccttag caagtgttgt ttgagagata ttcagtgact ctcgatattt
     3061 tcgtaggttg ctcatatgcc ctccatagta accacgaata aaaaatacaa tatgtacttc
     3121 tcaaatacaa gtaaaaatac acattgtgca tggatggttc cagtacagag cgtaataata
     3181 aggacatgaa aatgaaatgg tatgaactgg ctagatccag aatgaaagag ctcggcataa
     3241 ctcaagagaa gttagccgaa gagctaggta tgacgcaggg tggaattgga cactggttgc
     3301 gcggatctcg tcatccatct cttagtgata ttggtgtggt gtttaaatac cttggtattg
     3361 ataacatatc attcaaccac gacgggacat tttcacctgt tggcgaatac tcatcggccc
     3421 cagttaaaaa acaatatgag taccctgttt tttctcatgt tcaggctggg atgttctctc
     3481 cagaactcag aacctttacc aaaggtgatg cggagagatt ggtaagcaca accaaaaaag
     3541 ccagtgactc tgcattctgg cttgaggttg aaggtaactc aatgaccgca ccaacaggtt
     3601 ccaaacctag ttttcctgac gggatgttaa ttctggttga ccctgagcaa gctgttgagc
     3661 ccggcgattt ctgcatagcc agacttggtg gtgatgaatt taccttcaag aaactgatca
     3721 gggatagcgg tcaggtgttt ctacaaccac taaacccgca atatccaatg atcccatgca
     3781 atgagagttg ttccgttgtg gggaaagtta tcgccagcca atggcctgaa gagacgtttg
     3841 ggtaaagagg atagatggag tttaatggcc atgagtatca agcggtcaaa aagaagttca
     3901 tctattcatt gaaagcataa ggccatctga gtatatccgt aatgatctgg atattgtcta
     3961 taacatcaat gaccggacga tagatatcgg tgagcagcgt cctgtatggc agggtgaggc
     4021 aggtgaaaaa acgtcctgcc atcaacaaga atcaagtgca tccgctctct ggacagatgg
     4081 aaaatttatt ggatgcaaaa agatatgaaa taacacctgt atagcacgga gctttctctg
     4141 accgatgcac ttgaagttgt tagggctgac ccagcctgct gtttctttag gtaagagaaa
     4201 agagatttag gagatgaaag gtcgcagagg tgcggccttt tttattgaga gtggatcttg
     4261 aacggaaatt ctcaagactt gagtcttgca tgcaaatcaa ttcctggata aactcgatct
     4321 gagtcaataa cttagagaga gagcaaatgg caaagtcaaa cgttagcgtg caggcattca
     4381 aggacttcct tgaagagctt atgtcgctga acataatgaa ggaggccacc gctcgaaatt
     4441 taaaaaactc atccgctcgc ctcttaacgg tagtccaaga agaggaaatg ggtgatgtta
     4501 ctcagcttga tgtgaatgag cttgccgagc gatacatcaa cgcaactgag ccgaagccta
     4561 gcgacagcag cattactgca tataaaagcc gcatggaaag tgcaatcaaa aagtttgtag
     4621 ctttccagtc tggtgaagaa atcccataca ctccgattga caaagaatcc agtgaggaaa
     4681 aagatttgac tggcgaacca acaaaagtcg aaggcaaggc taatgcactt catacctatg
     4741 atcttccagt agttcttcga cctgaatcag gggttacagt aacgattaaa ggcattccta
     4801 acgatatcac aaacgaagaa gccgaacgca tctcttcaat tctgaaggtt tacgttcggc
     4861 ctcaataatg caaaagcatt cagcacaatg tccaactccc cagtccgaca ttgatgctgt
     4921 ttaaccagag cctccaaaag gagcccttgt taaggtacac aatatttgcg atgtaacctt
     4981 agcgcgtctg gtacattttt tcaagcggtt gtagggctgc cgccaatatg agaaaacaca
     5041 gatgtctaca tacaatttga atgaccaatt cgatcgagaa gttcatgtga atgcctatga
     5101 acggataagg catggaaatc ttgaacatgt gtgtgagcat tatcgctcaa ggccacaccg
     5161 ctaatcatca acccggcctc catgccgggt tttcttttcc tctcgcccaa aaaaacacat
     5221 aaccaattgt atttattgga aaataaatag atacaactca ctaaacaacg caattctgat
     5281 ctctccttac atcgccgagg caatacaccc acgctaaaaa acaatactat taaatacaaa
     5341 gcgttgtaaa aaaacgcccc gttttagaac aaattgtatt gaccaagtga agtacatatc
     5401 gtactattta accatcagca ggacgctgga agccaaatgg aacagactgg caggctcttt
     5461 aaacaacgtc gactctcgac tacgtggctg aaaagccaga tcacccaacc acataagctg
     5521 tgggatgcaa tgccgaagca accgtctcag gaggagcttc gagattgcat cgccaaagtt
     5581 tattcgggag gaatctatgt ccagaaaaac agaatttaaa ggcaccgcag cttctcgccg
     5641 tagagctcgt cgcgcaaacc tgcaaagtca ggaggcgatt agctccgaca aactacacag
     5701 gccaacccca tcacgagtgg tcttacaatg caaacgcaaa ccagcaatga gagcagaagt
     5761 aataacactg acaacgttga ccagaaaata tgaaggctca acttgtcttc caaatgtagc
     5821 tctttacgcg gcaggctacc gtaaatccaa acaactgaca gcaagatgac ttgtgttggt
     5881 cgccagaaaa tgaaattagg cagcaaacca cttatttgag gtgatatatg gaatttcatg
     5941 aaagtgcgat ttgtgatttt cgcgctaacg caaattcagt aaaaccacag ccaattgcag
     6001 ttctttttaa aacaatgggt gcgtgggctg ttttatgctt cgccgctgac gacactgacg
     6061 caagaatggc aataggacaa gagatggaga tggacccgac aaacgatgaa ttcataattt
     6121 atggcgctcc atctaattac ttacttgata cctgcaacat ttacaacaag gctgcctgat
     6181 ggtggccttt atttttggca taaacaacag aataaacaca gcactgtgta ttcattccaa
     6241 cgagtgaata cacggagcaa tgtcgctcgt aactaaacag gagccgactt gttctgatta
     6301 ttggaaatct tctttgccct ccaatgtgag ggcgattttt tatctgtgag gatatgaaca
     6361 gatgtcaaac atcaaaaaat acatcattga ttacgactgg aaagcatcaa tagaaattga
     6421 aatcgaccat gacgtaatga cagaggaaaa acttcaccag attaataatt tctggtcaga
     6481 ctctgaatac cgactcaata aacacggctc tttattaaat gctgtattaa tcatgctggc
     6541 gcaacatgct ctgcttatag caatttcaag cgacttaaat gcatatggtg ttgtgtgtga
     6601 gttcgactgg aatgatggaa atggtcagga aggatggcct ccaatggatg gtagcgaagg
     6661 aataagaatt accgatatcg atacatcagg aatatttgat tcagatgata tgactatcaa
     6721 agccgcctga gcgcggcgtt accgcatacc aataacgctt cactcgaggc gtttttcgtt
     6781 atgtataaat aaggagcaca ccatgcaata tgccattgca gggtggcctg ttgctggctg
     6841 cccttccgaa tctttacttg aacgaatcac ccgtaaatta cgtgacggat ggaaacgcct
     6901 tatcgacata ctta
//
LOCUS       DAZKFZ010000232         6782 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000232 DAZKFZ010000000
VERSION     DAZKFZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(678..1484)
                     /locus_tag="ACHOQW_004184"
     CDS             complement(678..1484)
                     /locus_tag="ACHOQW_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9575148.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1485..1790)
                     /gene="ccdB"
                     /locus_tag="ACHOQW_004185"
     CDS             complement(1485..1790)
                     /gene="ccdB"
                     /locus_tag="ACHOQW_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFP9575149.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1792..2010)
                     /gene="ccdA"
                     /locus_tag="ACHOQW_004186"
     CDS             complement(1792..2010)
                     /gene="ccdA"
                     /locus_tag="ACHOQW_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFP9575150.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2553..3152
                     /locus_tag="ACHOQW_004187"
     CDS             2553..3152
                     /locus_tag="ACHOQW_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9575151.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            3149..3769
                     /locus_tag="ACHOQW_004188"
     CDS             3149..3769
                     /locus_tag="ACHOQW_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575152.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            3766..3966
                     /locus_tag="ACHOQW_004189"
     CDS             3766..3966
                     /locus_tag="ACHOQW_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575153.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            4374..5351
                     /locus_tag="ACHOQW_004190"
     CDS             4374..5351
                     /locus_tag="ACHOQW_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9575154.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5636..6376)
                     /locus_tag="ACHOQW_004191"
     CDS             complement(5636..6376)
                     /locus_tag="ACHOQW_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9575155.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6497..6727)
                     /locus_tag="ACHOQW_004192"
     CDS             complement(6497..6727)
                     /locus_tag="ACHOQW_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFP9575156.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         1686 a         1784 c         1735 g         1577 t
ORIGIN      
        1 acaatttttt agattgtcac actaaataaa aaagagtcaa taagcaggga taactttgtg
       61 aaaaaacagc ttcttctgag ggcaatttgt cacaacacct tctctagaac cagcatggat
      121 aaaggcctac aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa taattataaa
      181 aatatccccg tggataagtg gataacccca agggaagttt tttcaggcat cgtgtgtaag
      241 cagaatatat aagtgctgtt ccctggtgct tcctcgctca ctcgagggct tcgccctgtc
      301 gctcgactgc ggcgagcact actggctgta aaaggacaga ccaaacatca tggttctgtg
      361 ttcattaggt tgttctgtcc attgctgaca taatccgctc cacttcaacg taacaccgca
      421 cgaagatttc tattgttcct gaaggcatat tcaaatcgtt ttcgttaccg cttgcaggca
      481 tcatgacaga acactacttc ctataaacgc tacacaggct cctgagatta ataatgcgga
      541 tctctacgat aatgggagat tttcccgact gtctcgttcg cttctcagtg gataacagcc
      601 agcttctctg tttaacagac aaaaacagca tatccactca gttccacatt tccatataaa
      661 ggccaaagca tttattctca ggataattgt ttcagcatcg caaccgcatc agactccggc
      721 atcgcaaact gcacccggtg ccgggcagcc acatccagcg caaaaacctt cgtgtagact
      781 tcctttgaac tgatggactt atgtcccatc aggctttgca gaactttcag cggtatacca
      841 gcatacagca tgtgcatcgc ataggaatgg cggaacgtat gtggtgtgac cggaacagag
      901 aacgtcacac cgtcagcagc agcggcggca accgcctccc caatccaggt cctgaccgtt
      961 ctgtccgtca cttcccagat ccgcgctttc tctgtccttc ctgtgcgacg gttacgccgc
     1021 tccatgggta ttttcagtgt tgccaccatc gtctgcagct ggctgacgta ccaggagtca
     1081 gagagcggaa ccagccggtg agtctgctga ccggcgggca ttctccccgc cgtcctggca
     1141 gctttttcgg tccgttgttt cagggtcgca agctgcacaa acggatacgg aggcgcaagc
     1201 gaaaaatccc cccgcgtcag cgccagtgct tcattaatgc gtgctccggt gttccacagt
     1261 gtggccagca gcatcttgcg gtgcagattc gggacgtaat ggagcagggc actcacttcc
     1321 ggagccagca gatattttgg cagttcatca tggaccatcg acatctggcg aagtgccaga
     1381 gctgccggat aatcaatggc aacaggcagc gatgcaggct gtccggcaga atacactgcc
     1441 ggtaccatga ctgcagactg gctgtgtata acggagcctg acatttatat tccccagaac
     1501 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
     1561 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
     1621 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
     1681 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
     1741 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagtc
     1801 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
     1861 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     1921 tgtgcttacc agaccggaga tattgacatc atatgccttg agcaactgat agctgtcgct
     1981 gtcaactgtc actgtaatac gctgcttcat agcacacctc tttttgacat acttcgggta
     2041 tacatatcag tatatattct tatgccgcaa aaatcagcgc gcaaatacgc atactattat
     2101 ctggctttta gtaagcctta tgtattttac ctttcgttat gttaaccaat aaaaattaaa
     2161 atctgcctta taaaaacaaa gcgtaattac cgcattcccg tttcgtatgg atccgacttt
     2221 atttgaataa acgactttcc ggaagtgtac cacctgagaa aatcatcata tcgctgtttt
     2281 ccgcccctgc gcggcgcatc acagtccaga gatacaacac aacatatagt gccatgggca
     2341 actgaatgag gcactgcatg ctgtgctgac gggatgtaac gctgtgcgga gggagaggaa
     2401 gatccgtaac caccagaaac cggatctgac caggaaaaga gaaagagtgt atgtaagttc
     2461 tgtactgtat gtatgaattg tattgtatgt attgtgtatt tttcataccg ggcatacgat
     2521 ggcgccattc cctgagaaac cggagccaga ccatgacatc cctctccccg gacaccgtcc
     2581 gccgcattga agacgccgcc gccgccctga tcgccgccgg caccccgaac ccgaccaacg
     2641 agcaggtccg tcagcacctc ggcggcggct ccctgtccca catctccccg gtgatgcgcg
     2701 cgttccgcgc ccgccagcgc gagcaggcca cgcccctgcc gccggagctg gcgcagctgc
     2761 tgaccggcca gctggggctg ctgtggcagg cggccgtgaa acaggctgaa gccggggccc
     2821 tggcggcgcg cgagcaggcc gacgacgaca ttgcccgggc cgacaaggag cgggatgagg
     2881 cgctggcgaa cgtggccgcg ctggaaagcg agctggcggt gctgcgcgag gtggtggccg
     2941 aacgtgaccg cctgctgcag gaggtacgcg agctacgcgc ggaggcgctg ccgctgcgcg
     3001 agcaggtggc ccgcctgacc gccaccggcg aacacctggc ggcgcagctg caggacacga
     3061 aggcggagct gaaggaagcc cgggaggacg gccgccagct tcagaccgaa ctgctggctc
     3121 tggcccgcca ggacgggaag gtgaagaaat gagacaggaa agagtattac gtataaaggc
     3181 atttcgacgg gcactggagc ttggtggcgg tgaacgggct gacgggtgtc gtcattttcc
     3241 ccgctggaaa acggaactga ggaatttccc gcacagcagc tgcgatttag ccagcaatac
     3301 tctggcgtac tacctcaaag aacgagagag atgccatccg tgcatcattt ttatggaagg
     3361 gaacgctggc tttcatgaag cggaaaactc aacagttcac gggcatgtca tcgtattgct
     3421 ggacggagag tacatcgatt tgacgctgga tcaattcgac gaatatccgg actacattcc
     3481 ttacgaacct gtcgaatctg acgggcagat tggtaagctg ttgcgtaata tcatgaagca
     3541 tgaggacccg gtaaaaaccc gccagattga tctcgatggt ggcgaagagc tgtatgcctg
     3601 gttacgcgat accgccgacg aagtgctggc agcagatccg gaatggcagg cccgggaacg
     3661 gtcgatagaa gaagcaaggg aagccgctat tacggtgttc ccgtttctgt ctgacgtgca
     3721 aaaaactgag tgcgaacagg gcccggacag tcaggaggct gccagatgaa gatttatcat
     3781 tacactgacc tgaacggcct gaagggcatc atcgaatcag gctcactgtg ggcgacgcac
     3841 ttttcctttc tcaatgacag taatgagctg actcacggca tgaactgcct tgagaatgcc
     3901 ctgcagtacc tgagggacga ctttaacccg aaaaccctta agttcatcga acaggcaccc
     3961 ttataagcgg ttgtgtatgc tgtttccctg gaatgattta tccacatatc cggaggctcg
     4021 atccagtaaa cagatccatg aatgatcaac aaaggatcca ttaaagatcc ccataccgct
     4081 gcaaaccttg tcactcatgg gccgggacca cgatcacata agcagtggca tgttactgat
     4141 aaactgtaac atgctaatga taagctgtat tcagtaatcc atatactgaa gtaagttaat
     4201 gacataaact atggtcagta cgccagactc agctgttaaa tacaggctgc aggtttttct
     4261 tcagtcagtt agcggggctc tgacacacga tttgctgttt attcttttac tgtccacagg
     4321 caggaggctt tctggaaaac gaaaattcag acatcaaaaa actgttcggc gaggtggata
     4381 agtcgtccgg tgagctggtg acactgacac caaacaataa caacaccgta caacctgtgg
     4441 cgctgatgcg tctgggcgtt tttgtaccga cccttaaatc actgaagaac agtaaaaaaa
     4501 atacactgtc acgtactgat gccacggaag agctgacacg tctttccctg gcccgtgctg
     4561 agggattcga taaggttgag atcaccggcc cccgcctgga tatggataac gatttcaaga
     4621 cctgggtggg gatcattcat tcctttgccc gccataacgt aattggtgac aaagttgaac
     4681 tgccttttgt cgagtttgca aaactgtgtg gtataccttc aagccagtca tcacgcaggc
     4741 tgcgtgagcg catcagccct tccctgaaac gcattgccgg taccgtgatc tccttttccc
     4801 gcaccgatga gaagcacacc cgggaataca tcacccatct ggtacagtca gcctactacg
     4861 atactgaacg ggatattgtt cagttacagg ctgatccccg cctttttgaa ctgtaccagt
     4921 ttgacagaaa ggtccttctc cagcttaagg cgattaatgc cctgaagcga cgggagtccg
     4981 cccaggcact ctacaccttt atagagagcc tgccccggga tccggcaccg gtatcgctgg
     5041 cgcggctgcg tgcacgcctc aatctgaagt ctcctgtatt ttcccagaac cagacggtca
     5101 gacgggcaat ggagcagctg cgcgagattg gatatcttga ttacacggag atccagcggg
     5161 ggcggacaaa actcttctgc attcactacc ggcgtccccg gttaaaagca ccgaatgatg
     5221 agagtaagga aaatccgttg ccaccttcac ctgcggaaaa agtcagtccg gagatggcgg
     5281 agaagcttgc cctgcttgag aaactgggca tcacgctgga tgacctggaa aaactcttca
     5341 aatcccgctg aacataaact gtagtcagtg aagagtgttc ctttactgac tacagcttat
     5401 attatcaggt gcagtgagtg gtctgctcac tgcagtttat attcagtttc ctgcagtgct
     5461 gcctgtagct gagctgtcat ctgccggtcc cttacgtgag tcaccccgta acctgatgct
     5521 gaggcattgc tcccttcata aaacatgact tactcactac agcttatata catgctccag
     5581 cttatgttat gtctgttctg ctgaccacag cttatataag gaagcgcgta tgccgtcacc
     5641 tcaggggagg cagtgtacgc aggatctccg cagcatcccg cccgtcacct gtgaaaggca
     5701 ctgccagcgt ggcagccata tccagcgcaa acactctggt ataaacctcc atcgaacgtg
     5761 gatccctgtg acctgccagg gcctggatga ctttccgggg ctggcggtga tagagcatat
     5821 gcatgatata gctgtgccgg aaggtgtgtg gtgtgaccgg aatcgaaaag tgtactccgt
     5881 cagcttcggc ccgtctgaca gcctgcttca gccagttgcg catggtttcg tcggtcacgg
     5941 cccataatgg ttcacgacga cggggccggg tggtgatcat ccagctttcc atctgcctga
     6001 catagcttat atccgtcagc ggaaccaggc ggacttcatc ttttggcggg cgtccgcgtc
     6061 gcgcacgcac tttttcggac aggatccgca caaacggcct tgctccatcc aggtcaaatg
     6121 attccggcgt cagcatccgg gcttcgccaa tacgcattcc ggtattccag agggtggcga
     6181 acagcatatg gtgacgctga tccggcatat agaaaagaag ggcactcact tccggggcca
     6241 gcaggtaggc cggtgtggcc cctgtggaag tggccattct tctcagggag agcgctgttg
     6301 caaaatccac ccccggcgca atgggtaaca ggaagacgcg ttctggtgaa ttctgcaggg
     6361 gaatgacatt gttcatgagt tatttttcac gtagcacacg cactgtttat ggatacaggt
     6421 attctgacct gaacgcccgg atccttccac gacaataaat cgcagcgtgc ggatcctttt
     6481 gtttgcttat cacgcattat tgcgggtaaa accaaatacg taaaggtacg ttttgcagca
     6541 gggatcccgt attaagccag tcgtggcgcg ggctgagggc tggtttgccg tttttgggag
     6601 aattggggta atgccaggac gctaatgatc tatcaatgga tggatgtaag tgacaattga
     6661 aaactgaaaa atgcgctatg tagattctga cagatttatg cacctgaaat acgttcaaaa
     6721 tccacatagc gtaactctga ttttccttac cagaaaaaag agggggaata accagtatct
     6781 at
//
LOCUS       DAZKFZ010000233         6770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000233 DAZKFZ010000000
VERSION     DAZKFZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1881
                     /gene="espL4"
                     /locus_tag="ACHOQW_004193"
     CDS             <1..1881
                     /gene="espL4"
                     /locus_tag="ACHOQW_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFP9575157.1"
                     /translation="FEEYLMEILPQRSDALDKKELNLNSDRKEKEFPRVKLNGQCYFP
                     GRPQNRIVCRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSR
                     PEGRCVAACGDFGLVMKAYFDKMESNDLSVMAAILLVDNHALTVRLRIKNTTEGCIHY
                     VVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPAL
                     PKEQLLMLKGTVDEITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEMLTAY
                     NSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFL
                     AISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADML
                     KIVLDALSLLIRTCHLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSL
                     AQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKN
                     MKPLLLANNSNEYPGLFSAIQHKQQNVVEMVYLALSDHARLFGFTAEDIMDFWQHKAP
                     QKYSAFELACELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHT
                     VR"
     gene            complement(1850..3586)
                     /gene="aceK"
                     /locus_tag="ACHOQW_004194"
     CDS             complement(1850..3586)
                     /gene="aceK"
                     /locus_tag="ACHOQW_004194"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP9575158.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3770..5074)
                     /gene="aceA"
                     /locus_tag="ACHOQW_004195"
     CDS             complement(3770..5074)
                     /gene="aceA"
                     /locus_tag="ACHOQW_004195"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP9575159.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5104..6705)
                     /gene="aceB"
                     /locus_tag="ACHOQW_004196"
     CDS             complement(5104..6705)
                     /gene="aceB"
                     /locus_tag="ACHOQW_004196"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP9575160.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1718 a         1695 c         1630 g         1727 t
ORIGIN      
        1 tttgaagaat acctaatgga aattttacct caacgctcag acgctcttga taaaaaagaa
       61 ttaaatctaa actcagatag aaaagaaaaa gaattcccaa gagttaagct taatggtcaa
      121 tgttattttc cggggcgccc ccaaaaccgt atagtatgcc gacacattgc tgcacaatat
      181 attaatgata tttatcagaa tgttgactac aaaccccatc aagatgatta ctcttcagct
      241 gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc agactttggc gttgatttcc
      301 agccgtcctg aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt tatgaaggca
      361 tattttgaca agatggaatc aaatgacctc agtgttatgg ctgccatatt attggtagat
      421 aaccatgctt tgacggtccg gctaagaata aagaacacaa ctgaaggatg tatccattac
      481 gtggtttcgg tttatgatcc taatgtaact aacgataaaa taagaatcat gagcgaaagt
      541 aaagaggata ttaaacacta ttcactgatg gattttatga atgtagatta tagcctcctg
      601 aaatggtcaa atgatcatgt tattaaccaa tctgttgcaa taattccagc acttccaaaa
      661 gaacagctat tgatgctaaa aggaactgtg gatgaaataa cccctccatt atcacctgca
      721 acgatgaatt tgctaatggc aattggtcag aatcaccaac ttaagcaact gatgattcaa
      781 ctccagaaaa tgccagaact acatagaaca gaaatgttga ctgcctataa tagtattaac
      841 ctccccggtt tatatttggc tataaattat ggtaatgcgg atatcgttga gactattttt
      901 aattcattgt cagagccagg atatgaagga ttactctcga aaaaaaatct catgcatatt
      961 ctggaggcaa aagataaaaa tggtttttct ggattatttt tagcgatatc acgtaaggat
     1021 aaaaatgttg taacctcgat tctgaacgcc ttacctaaac tggccgcaac acatcattta
     1081 gataacgaac aagtgtataa attcctgagt gccaaaaata gtacgtccag ccatgtttta
     1141 taccatgtta tggcgaatgg tgatgccgac atgctgaaaa ttgttttgga cgcgttatct
     1201 ttgttaattc gcacatgtca tttgactaaa gaacaggtac tcgatctcct gaaggcaaag
     1261 gatttttatg gttgcccagg actatacctg gcgatgcaaa atggacatag cgatatcgtg
     1321 aaagttattc ttgaagcatt gcccagcctg gcccaggaaa ttaacatttc agcttccgat
     1381 attgtagatc ttctgaccgc taaaagtctt gcgcgcgaca cgggtttgtt tatggccatg
     1441 cagcgcggac atatgaacgt tattaatact atttttaacg cattacccac tctgtttaat
     1501 acgtttaaat tcgataaaaa aaatatgaag cccctcctcc tggcaaataa ttctaatgaa
     1561 tacccaggtt tgttttcagc gatacagcat aaacagcaaa acgttgtaga gatggtttat
     1621 cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatggatttt
     1681 tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttgtga attgggtcac
     1741 cgggttattg ctgaattaat ctttaataca ttaaataaga tggctgaaag ctttggcttt
     1801 acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca
     1861 tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca tcttccacat
     1921 gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt
     1981 gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccaatgg cgaaactctt
     2041 ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg
     2101 ggtagcgcgg cggcgggata tcgcggaaat tcacttccgt catgtagcaa atttcatcgt
     2161 aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc
     2221 ccgggaaaat gttagcagcg gcaagctggc gaatggcatt gccgtactct tcaatggcgt
     2281 cgcgcagctg ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct
     2341 caatataaag atggcgaatc acaatttgtt cgccgagatc ggtgattttt tccgctgctt
     2401 cctggagcag taattccatt aatgccgggg aaatatgccg cttctccagc acaaagtttt
     2461 caaactcctg ggtatccgcc attcggccca cgcgatcgtg ctctttcacc agttgatagc
     2521 aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct ttgatgactt
     2581 taaatacacg gtcaaagccc ggcagcgtga acaccaacat caccatgcca cgaatacccg
     2641 gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg cggtagcttt
     2701 cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcggtg gttttacctg
     2761 gcagaatttc ccgtaaccac tcgaccagcg ctgcgggcag cggcgcataa accataaaat
     2821 aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcaga caggtatcaa
     2881 taaacagttc gccgtcatcc gtctggtgga tcggcaataa aaatggcaat gtgccggaag
     2941 gcgttattag tttgcccacc agccaggcgg ctttattacg gtaaaacagt tcgttcgcca
     3001 cctgtaaatg acttttggag aggttttctg gccccaacgt ttccgtcaga tggcgaataa
     3061 tgtaatggat gtcacggctt ttattctgcc agtgcagacg caacggcagg tcgctgataa
     3121 cgcgcatcag cagagattcc cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg
     3181 gaatggtacg aaagcggcgc tctggctggg agctaaagat aaaaagccgc tcgggagtaa
     3241 gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga
     3301 agcgcgggta atccggcaac agccgggtgt aatgctcttt gacgcgcagt aaaaattccg
     3361 cgtccgtgct ttggccgttg gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat
     3421 gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag tcggcttgtt
     3481 cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc
     3541 cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc
     3601 aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg
     3661 ttgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg
     3721 cggcgtatat gccttatccg gcctacagtc agcaacgttt gtaggttgct tagaactgcg
     3781 attcttcggt ggagccagtc agcgcggtaa ccgacgacgt gccgccctga ataatggtcg
     3841 tcactttatc gaagtaacct gtacccactt cctgctggtg agatacgaag gtgtagccat
     3901 ctttcgcggc ggcgaattcc ggctgctgca ctttctcaac atagtgcttc ataccctcgc
     3961 cctgggcata ggcgtttgcc agatcaaaca tgttgaacca catgctgtgg atacctgcca
     4021 gggtgatgaa ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag
     4081 tgttgtcgtc gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt
     4141 tgcccggata tttcgcgtgg atagcttccg caaagcgacg cgccagttcc agatccggcg
     4201 tggaggtttc acaccatacc aggtcagcat atggcgcata cgccaggcca cggctgatcg
     4261 cttgctcaat gcccgcatga gtacggaaga agccttcact ggtacgctcg ccggtaataa
     4321 attcgctgtc atacgggtcg caatcggagg tgatcagatc cgccgcatca gcatcggtac
     4381 gtgcaaccag cagggttgga acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt
     4441 tctgaatagc ttcctgagtt ggcaccaaaa ctttgccgcc catgtgaccg catttcttca
     4501 ctgacgccag ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc atcgctttca
     4561 tcagttcaaa ggcattcagg acaccgccaa aaccggcttc cgcgtcggca acgatcggca
     4621 ggaagtaatc gacatagcgc ggatcgcccg gctcaatgcc cgcggaccat tggatctgat
     4681 ccgcacggcg gaaagtgttg ttgatccgct ccaccacagc tggcaccgag tttgccggat
     4741 agagcgactg atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc
     4801 ccgacagata gaccgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca
     4861 gtgcaccgag gctgttgatg tagccttttt tcgactcgcc atgcagcaga cgccacattt
     4921 tcgccgcgcc cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt aatttcacca
     4981 catcttccgc gctgtatggg cgagtaattc cttcccaacg cggttgagtc cactctttct
     5041 gtaattcttc aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt
     5101 ggtttacgct aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt
     5161 ggtgatctgt tccatcaagc gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc
     5221 gcccagttcg ctggcaatga ctttcatctc ttcgcccagc atctggcgga acaaggcttt
     5281 agttaccggt ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agattgaggt
     5341 acgggaaatt tcagccgtcg ccgcatcttc catcaggcca taaatcggca cgcagccgtt
     5401 gccggagatc catgcttcga tgtactgcac agccacgcga atgttggcgc gcataccttc
     5461 ttcggtgcgt tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg
     5521 ttcgcgcatc acttcaagct gatttttacg ggagccgaga atgtcgttga ataccgccat
     5581 tgccgtgtcc gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc
     5641 cagcgattta tccgctttta ctttgttgag cacctggtta ttgtgctctt catctttgct
     5701 cggaataaac gccgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat
     5761 caacaggcgt gagtaagcat tcaggaatgg tttatccatc gttactgcct gtctgtctgg
     5821 caggacgcga tcgggatagt ttttcaacgt tttgatatag ctgaagatgt aatcccaacg
     5881 accgcagttc agaccaacaa tatggtcacg cagcgcgtga aggatttcat ccatctggaa
     5941 cacggcgggc agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa
     6001 gcgatcttct gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt
     6061 tttcggcaga tagaaatagg gaccactgcc ctttgccaac agtgcctgat agttgtggaa
     6121 gaaatagagc gcaaaatcaa acaggctgcc ggggattgcc tcaccacgcc aggtgacatg
     6181 tttttccggc aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag
     6241 ctggtaaatt ttgcctgctt cattggtgta actgatggtg ccgttaaccg catcacgcag
     6301 gttaatttgc ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc
     6361 cataaagact ttcacattgg cgttgagcgc gttgatcacc atcttgcgct ctacggatcc
     6421 ggtaatctct acgcggcgat cttctaagtc cgcaggaatc ccgcgaattt tccagtcagc
     6481 atcacgaatg gaagctgttt ccgaaataaa atcaggcaac gttccgttat caatatcttg
     6541 ctgctgctga atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag
     6601 ctcagtcaga aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg
     6661 ccttatgaaa gccagttcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt
     6721 catggatccg aaaacttccc cactgtgaac gaaggatcgt tgtgcgcttt
//
LOCUS       DAZKFZ010000234         6758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000234 DAZKFZ010000000
VERSION     DAZKFZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(370..642)
                     /locus_tag="ACHOQW_004197"
     CDS             complement(370..642)
                     /locus_tag="ACHOQW_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9575161.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(648..1199)
                     /locus_tag="ACHOQW_004198"
     CDS             complement(648..1199)
                     /locus_tag="ACHOQW_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFP9575162.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1196..1948)
                     /locus_tag="ACHOQW_004199"
     CDS             complement(1196..1948)
                     /locus_tag="ACHOQW_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFP9575163.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
     gene            2882..3142
                     /locus_tag="ACHOQW_004200"
     CDS             2882..3142
                     /locus_tag="ACHOQW_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9575164.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
     gene            3139..3696
                     /locus_tag="ACHOQW_004201"
     CDS             3139..3696
                     /locus_tag="ACHOQW_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9575165.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            3693..3914
                     /locus_tag="ACHOQW_004202"
     CDS             3693..3914
                     /locus_tag="ACHOQW_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575166.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAALQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            3914..4237
                     /locus_tag="ACHOQW_004203"
     CDS             3914..4237
                     /locus_tag="ACHOQW_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFP9575167.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            4251..6584
                     /locus_tag="ACHOQW_004204"
     CDS             4251..6584
                     /locus_tag="ACHOQW_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP9575168.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
BASE COUNT         1586 a         1634 c         1717 g         1821 t
ORIGIN      
        1 atatttcgtt tcgagcgctc agttcatttc taaataattt gcaaaattat tttgggtact
       61 ttcgcctcta cacttccttc gggtggttcc acaataatgg atctcaccgg cgtttgatat
      121 tttataatct aaccccttag cgcgcgcagc cccccacgcc tgctcgcttc gcttaacaga
      181 ctggttttca tgcattccgc aaatcgtctc agaagccacc acacaagggc tttcgcgtca
      241 aaaatggtgc atgagaatca tgcgttttca tgcgctatag acatgcactc atgcgctctc
      301 aggccagcca gggaaaaggc gtaaaaaatc ccagtactgg accgggatta cgtgggcttt
      361 ttttgctaat cagacaggaa tttgctgacg gcttgacagc tttgacgcgg agccatagcg
      421 attgagagtt tgctgttgtt tttccggcat ttgtgctttc tctgattcct gttcattgcg
      481 ccggggcttc atgattatcg tatccacact ttccagtgcg gtaaatgtgt aggaacattc
      541 aagattctgg cactgatacc atgagcgttt aaccgatggt gcttcatagg cggaggttct
      601 ggcgtgtgcc gtcgtaccgc attcgggaca tttaagtgcc attaatatta ctcctgcatg
      661 ccgttaatgt aatccagacg ttgctgaaag cgcagttgtt gtgcaggggt gtaagagctc
      721 tggcaatcac gaaccatcat ggggtcaggc atcacaccgg attcatccag aatcatacga
      781 taatccccgg caatttcagg cggtgttatt gccagctggc gaaccagagc attgcgtaat
      841 atacgggacg caacgtcaac ccctccgcgc cccttcagga acggagccag tgcagatgtc
      901 agggctgctc cattcgcctg cataaaattg ctgagagcgt cttctgtgca ggcctccatc
      961 agttgatgct gcgagtaaat gcattcgcgc gcagcacagt taatttgcca tttcagcaca
     1021 tccagatgtt cccgctgtaa ttcgagctgt cgagcataat ctgctggttt ttccatggca
     1081 gtgaggagtg attctatctg ttgttcaacg gcttttcggc tctcactatg tccagcccac
     1141 agaatacggg catttctaaa actgataagc gcttggtctg gagtgactcg ggccatcata
     1201 ctgctttctc cttctcagca cgttcctgac gaaccttcat tttatgcata gctgctggag
     1261 agagtttatt actgaaatcc agacgggccg cataatccgg ggcaactccc gccagtttaa
     1321 acacggggtc ctgttcgggc atcgcatcgt tagtcagagc cggtctggtg atatgtttat
     1381 gaataaaatc tttcagcagt gtgtccgggt cattaactga atgcaccaca ccgacaactg
     1441 cgctggcttc cctgcccatt gttgatttga gcaggcttag tgtctggatt agtgcttttc
     1501 catgcgactg cataaaatct tcccagattt gttttgcgcg aatacccaca agggtgtcgt
     1561 gcgcgtggat atactttccg gctaattccg cagcctcctg cggcaaaagt gcattttcgc
     1621 tttcctgagc cgccagcagc tcatcaaaat cctcaagcgt ttctcgtccc agcgcaattt
     1681 ccgtgcgcag ttttttcatt tcctttgaga ctacgccctg actttcacga aatagcgtgc
     1741 gccactcctc attcagggcg ttcgtcgtgg cttccatttc agcgcggcgc tggcggattg
     1801 tcgcaatatt atccgctgcc gctttttgct gacgacgtgc ttcaagccat gcagatttgg
     1861 tggcattcac tttctcaagc gcctgttgcg tcgaaacagg gatagaaatt tcaacgatcg
     1921 ggttattgtt ctgttctggg attgtcatgt cagcacctag tggtttgtcg tggtgtcaat
     1981 tgtgctgtga tgcatacaaa gctgccattg aacgccattg tgcgagcgac aagacaaaga
     2041 ctgaaccgcc ccggacctct tttggctagc cagaaaaggc ctcgtttaac taaggctgtt
     2101 tcagacttac cttaactatt cactactatt cacttaggta aaaaaaatag gtaatacaga
     2161 aggttataag gtgaagggtt ggcatggcag tattcatcga ctgttcataa ctgttcacat
     2221 gggaataaaa ttatttatgg tattttttca taatgggtgg tttttttatt cctcgtagct
     2281 atttgttttt tgctcctcac cactattcac ccttattcag gagtattcaa caggcacata
     2341 aatagactgc atgtttttgc cggggaataa cattttcgcc tctggccatg gtgttgcatt
     2401 tcatactgat ttttataaaa ctagatctgt ccggttcttt atggattcat tcggatatat
     2461 tttacgattt aaggtgatgt tatttaatat caaggttttt atgcaggccc aatgtttata
     2521 agttggatgc gattcaaaaa tatgcccgga aattggggcc tgcaatgcaa ggcctcgtgt
     2581 agaagttgta ctgccttgct gtattggcgg tggggttagc actatcaggt gcaatgaccc
     2641 ataggatagt aaaccctggg tgaatggtag ttcctattaa tgctcctgta tttatagtcc
     2701 gcaggcatgg tatttattat gtcattttat atggtttaat cgggctttaa attccataaa
     2761 atcaatatgt gacgctaaat aaaaaatgcg tgcgcaggtg gtttatatac acataataag
     2821 gaactacctg aacccggata aattcatccg gactgttgtg gaaattaaag agggtagata
     2881 aatgcgtaat atttctgtgt ctgcgccagc tcctgctgcg ccattatttc ctgtatcgaa
     2941 ccatcatgag cgatttttac gcctgcctga ggtgatgcac ttatgcgggt tgtcccgttc
     3001 gaccgtttac gacctgatca gccgcgatgc gtttccgaag cagatcccgc ttggcggtaa
     3061 aaatgtcgcc tgggcacagt ctgaggtcag cgcatggatg gcggaccgca tcagcgcccg
     3121 tgggcgggga tgtgatgcat gatgatacct gcatacatta aataccttta ttctggcttg
     3181 cttactgtcg tcatttccag gtatagtttt tccgctgtcg caaaatcggc agccggaatt
     3241 ggcgttccgt ataacttatt ggcgacaatt gacgcccctt gcgtcttttt ttacgtcgta
     3301 gctcaggcac aaccattttc agggctgtgg tgtttatgct tacaccatgg ttctatcgag
     3361 ataatggtag tccgggcggg gcagccttcg ggctggccgg tttccaataa ggccggttac
     3421 gccaaccccg ttcgggctgc caccagtgaa attggcgttt ccggtggtag caataaccgc
     3481 tacttattgg aggctgccat catggctaca atcctcaccc cgtcacaccc gcaatatgtc
     3541 tttgtgtttg ccgcaatccg ccgtgctgac actcaccccc gcatctgtat gcttcgcact
     3601 gtctcctgcg atgagcgttc cgcgcgccgt ttgctagtcc gagattatgt actctccctt
     3661 tctgcacggc tgcctgccgg ggaggtgacg ctatgaacca gtttgagatt tcctacgacg
     3721 atgttgtaag gctgaaacat ttacgcaatg tgggtgagta tgtaactggc atggcagccc
     3781 tgcaggactg ttatgaaaag ccagcaggtg cccaatgcga acaactggtt tccctcattt
     3841 atctgatgac agagcaactg gatggagtgg tgcaacgctg ccatgacgac ctgatgaatg
     3901 cggaggtggc ctgatgatat gctgtgaatc tcttctggca cttcgtgccg ctctgtatcg
     3961 ccgtgccgtt gcctgtgcct ggctggctct aagcaatcat caggaacgtt attccggcct
     4021 gacgctcgct gaacttgaag atgccatagc ccgcgagctg gaagggttct atctgcgcca
     4081 gcatgggcag caaagagggc tggaaattgc ctgcgcgttg ctttcggatc tgatggagtc
     4141 ggggccgctt aaagcctgtc cggttctctc actgctcgga atgacggtca tggatgaact
     4201 ttgttcccgt cacctcaaca aaccagcgct gcactaagga gggccgcaca atgtcaggaa
     4261 tgaaagttag ccaggctgag aaagcagctc ggggtcactg gtcaagaatt ttacctgcgc
     4321 tgggcgtaaa tgtactgaaa aatcggcacc agccctgccc ggtctgtgcc gggaaagacc
     4381 gctttcgatt tgatgaccag gaagggcggg gaacgtggtt ctgtaaccag tgcggggcag
     4441 gtgatggcct ggcgcttgta agtaaagtac tggatgtagg cattagtgaa gcggcagaca
     4501 gaataaacgg cattatcgga aacctgctgc cagtatctca gggaatgctt gaatctggtt
     4561 ctcctgaaaa agaggacggg agaaaagctg cagcagtgct ggctgcccgt ttgtttgata
     4621 agtcccgcca gaccactggc aatgcctatc tgacgagtaa agggtttcct gcactgcctt
     4681 gccgggaatt aaccgctatg cataaagtcg gtggtgtggc atttcgcgcg ggagatcttg
     4741 tcgttccatt gtatgcagat ggagagctgg taaatctgca gttaatcaac gctaatgggg
     4801 gcaaatgctt ccttaaaggc ggtcaggtta agaatgcctt ttacctggtt gaaggtactg
     4861 ccaaagcagc caaacggctc tggatagcgg aaggatatgc caccgcactt actatcaact
     4921 atctgactgg cgatgctgtc atggtggcct tttcgtccgt caatttcctt tccctggcga
     4981 gcattgcctg cagtgagtac ccaacgcacc agataattat tgctgctgac cgcgatctca
     5041 acggtgcggg gcaaacaagg ggcgcagctg ttaccggggc ctgcaattgc acaatggcgc
     5101 tcccgcctgt gtttggtgac tggaacgatg cattcacgca aaacggcgaa gaagccaccc
     5161 ggcatgcaat tcatgaagta ataaaaccag ctgttgccag ccccttcgac acaatgagcg
     5221 aagctgaatt taccgcgctg agcgtcagcg aaaaagcgca gagggtagtg gatcactata
     5281 aaaattcact ggcagtagac ccgaacgggc agctcctttc acgctatgag gcgggggcct
     5341 ggaaagttat ctattacgcc gattttgccc gtgatgtcgc tgcgctgttt cagcgcctcg
     5401 acgcaccttt ttcatccgcg aaaattgcgt ctctcgtgga aaccctcaaa ctgatcgttc
     5461 cgcaacagca gaatccggcg cggcaactta tcggatttcg caacggtgtg ctcgataccc
     5521 ggacaggatt gttcagcccg cacgataaga agcactggtt acgtacgctg tgcgaggtgg
     5581 attacacgca gcccgttgac ggtgagtcac tggaaaccca tgccccggca ttctggcgct
     5641 ggctggatcg tgccgcaggt tttaatcctg aaaaacggga cattattctg gctgcattgt
     5701 ttatggtgct ggctaaccgt tatgactggc agctgtttct ggaggtcact ggccctggcg
     5761 gaagtggaaa gagtattctt gctgaaatag caaccatgct ggcgggtgaa gataacgcga
     5821 cctcggcaac cattgaaatg cttgagtcgc caagagaacg agctgcgtta ataggttttt
     5881 cactgattcg acttcccgac caggaaaagt ggagcggtga cggggccgga ctaaaagcca
     5941 tcactggcgg cgatgcggta tccgttgatc ccaaatatca gaacgcctat tcaacccaca
     6001 tcccggcggt catcctggct gtgaacaata atccgatgcg cttcactgat cgtagtggtg
     6061 gagtttcacg ccgaagggtg atcctgcatt tccccgacca gatagccccg gaggaacgcg
     6121 atacccagct caaagagaaa attgccagtg agctagcggt gattgttcgc cagcttatgc
     6181 agcgtttcag cgacccaatg agtgccagga cattgcttca gtcacagcag aactccgatg
     6241 aagcgctcac catcaaacgt gatgctgatt cagcttttga tttttgcggc taccttgagg
     6301 tcctacctga caccacgggc atgtttatgg ggaacgctaa tattgttcca cgtcagcctc
     6361 gaacttacct ctaccatgcc tatctggtct acatggaggc taacggctat aaaaatacgc
     6421 tcagtctgac catgtttggc aaggggctac cgttaatgct caaggaatat gggctgcagt
     6481 atgagaaacg acggaccaat caaggaatgc agactaatct ggccctaaga gaggaaagca
     6541 atgctgactg gttgccaaaa tgcgatgagt ttgcagcgaa ataactgacc taagaccggc
     6601 tgtgccggtc ttaaatccgc tacaggaaat tatctcaaaa tatatatcga taaaatttaa
     6661 aagttatatg ctaaggcgtc agcggaaaat gcatctaatt cattgttaga tgatgtttat
     6721 ttttgaattg atttaaacat cccatcgagt tgtaattt
//
LOCUS       DAZKFZ010000235         6654 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000235 DAZKFZ010000000
VERSION     DAZKFZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(316..2349)
                     /gene="betT"
                     /locus_tag="ACHOQW_004205"
     CDS             complement(316..2349)
                     /gene="betT"
                     /locus_tag="ACHOQW_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP9575169.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2478..3065
                     /gene="betI"
                     /locus_tag="ACHOQW_004206"
     CDS             2478..3065
                     /gene="betI"
                     /locus_tag="ACHOQW_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP9575170.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            3079..4551
                     /gene="betB"
                     /locus_tag="ACHOQW_004207"
     CDS             3079..4551
                     /gene="betB"
                     /locus_tag="ACHOQW_004207"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP9575171.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4565..6253
                     /gene="betA"
                     /locus_tag="ACHOQW_004208"
     CDS             4565..6253
                     /gene="betA"
                     /locus_tag="ACHOQW_004208"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP9575172.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1673 a         1815 c         1789 g         1377 t
ORIGIN      
        1 aactcaaatc aactcaaaat ataaacaaaa gcaggagaaa tacatttaat atacagccgg
       61 taaaagtggt aactcttatt aggtatccaa atataagaat cgtcttatta ataaatatat
      121 aaccctatag gactgaatcc attcagctaa tacaaaataa aatcgttaat taataaaata
      181 taaaatcatg aagtgataca agaggaattt caggatatga catgacaatc atatataata
      241 tatgcataat tttttacagc gagtcaatga ttcccgcgcc atatgcagac atggcgcggt
      301 tttatgcaat aacaatcacg cgtccgggaa catcacgcta tggcccggcg cttcacgatg
      361 gagatgaata aagttaaggt gccgctcgta ctggtcaaga atatcggtga tcacctgctc
      421 tttgctgtag tccatcaggt cgttgccctg gctgccttct aacaggaagg tttccagccg
      481 gtagtaggtc gatttcccgc tacgtgcgcg gtaggtaaag cccggcaccg agtattgctg
      541 cggccaaatc tgatagacaa agttttgctc ttcgcccata tgcaccaaca aatccagatg
      601 ccccaactgt tgtccctctt ccggcggcag gctttttagc tccacgtacg cgccgcgcaa
      661 ccgcaactcc tgcgccactt cttccattgc cgggtaacag accgtctcca tcatctgttt
      721 agtgtaacgc gtgcccggat aattcatcag gcgcgagaga cgttttttcc agctcaggcg
      781 atcctgaagc cccagcggtc gcggtgcggt atcgcggttg gcactttcac ggcggtaatc
      841 ttctaccttc agagatttat acaaccccgc catcacgaag aagatcacaa agctgaacgg
      901 cagccccata atcaccgtgg tgttttgcag cgcggatatc ccgttggtca tcagcatgcc
      961 gagcgtcagc aggccaatcg ccaccgacca gaagacgcgc agccagccgg gggcgtcgct
     1021 gttgatatct ttaagctgcg aggtgaaatt ccccagcacc agcgccccgg agtccgccga
     1081 ggtcacataa aacagcaggc cagtaatggt ggcgacggag gcgctaaagg taaacgccgg
     1141 atactgcgcc agcaggctgt agaagccgcg ctccggatgg accatcgctt cctcggcaaa
     1201 tgccgcgccg ccgtggatga tttcatacag cgcgctattg ccgaacaccg agagccataa
     1261 cagcgtgaag gtaaacggaa taatcaacgt gcccagcacg aactggcgaa tggtacgccc
     1321 acgcgagata cgcgccagga acaagccgac aaacggcgac catgccaccc accatgccca
     1381 gaagaagagc gtccagttat tcatccactc aaccgggcgg tcgaaggcaa aactgttgag
     1441 cgtcatgccc ataaagcgat tcacatagtc gccaacattc agcaccagtg cattaagcag
     1501 gaacgaagtg tcgcccataa acaacacgaa caggatcaat cccagcgcca gcgcgacatt
     1561 aagctccgat aacacgcgaa tgcccttatc gacaccggat gtcaccgaga tagtggcgat
     1621 tatcaccgac aaggcgatca gtgccgcttt tgccgccatc gaatcgggaa tatcaaacag
     1681 tacgctcaag ccatagttaa gctgcaccac accgataccg agcgtagtgg caataccgaa
     1741 gatagtgccg atcaccgctg caatatccac tgagtgacct atcggcccgt taatccgttt
     1801 accgaagatc gggtacagcg ccgagcggat ggtgagcggc aaattataac gatagctaaa
     1861 gtatccgagc gccatgccca tcagcgcata catcgaccag ccggttaagc cgtagtgaaa
     1921 cagcgtccag accatcgcct gacgcgcggc ctcaatcgtc tgtcccgcgc cttccggcgg
     1981 ctgcatatac tgcgttaccg gttcggctac ggaaaagaac atcagatcga taccgatccc
     2041 ggcagcaaac agcatcgccg cccaactcag caggctgaat tccggtttgg attgttctgg
     2101 cccgagcttc accgaaccaa aacgcgaaca agcgatacag accacaaaga caatatagag
     2161 cgttgccgcc agcagatagt accaaccgaa ggttttagaa acccagtcca gcgtgcggcc
     2221 aatccacagg gccgagaagt cgcgaaacag gatcgttgtc agggaaaaca acaaaatcag
     2281 tccggcggag gtgtaaaaca ccaccggatt gattttgtcc ttttccctgc tgtgtgaaag
     2341 gtctgtcatc cagtatcccc actgttattg ttaccattta aaatcaaatt cgtaacaatt
     2401 aagacacatt ttatattgaa cgtccaatca ataaccgctt taatagataa acaacgctga
     2461 tgaatggagt ggcaaaaatg cccaaattgg ggatgcagtc gatccggcgc agacaactga
     2521 tcgacgccac actggaagca ataaatgaag tgggcatgca cgatgcaacg atcgcgcaga
     2581 tcgcccgccg tgcaggcgtt tctacgggga tcatcagcca ctatttcagg gacaaaaatg
     2641 gtctgctgga agcaaccatg cgcgatatca ccagtcagct gcgtgacgcg gttttgaatc
     2701 gattacatgc acttccgcag ggcagtgcag agcagcgatt acaggcgatt gttggcggaa
     2761 acttcgatga aacgcaggtg agcagtgcgg cgatgaaagc ctggctggcg ttctgggcca
     2821 gcagtatgca tcagccgatg ctctatcgtt tacagcaggt cagcagccgc cgcttgctgt
     2881 cgaatctggt gagcgagttt cgccgcgaat tgccgcgcca ccaggcacag gaagcgggct
     2941 acggccttgc cgcgctgatt gacggattat ggctgcgcgc ggcactgagc ggcaaaccgc
     3001 tggataaacc tctcgcacac tcgctgaccc gtcactttat tactcagcat ttacccaccg
     3061 attaaccgag gagacgtgat gtcccgaatg gcagaacagc agctttatat acatggtggt
     3121 tatacctccg ccaccagcgg tcgcaccttc gagaccatta acccggccaa cggtaacgtg
     3181 ctggcgaccg tgcaggccgc cgggcgcgag gatgtcgatc gcgccgtgaa gagtgcccaa
     3241 caggggcaaa aaatctgggc ggcgatgacc gctatggaac gctcgcgtat tctgcgtcgg
     3301 gccgtcgata ttctgcgcga gcgcaatgat gaactcgcca aactggaaac cctcgacacc
     3361 ggaaaagcgt attcggaaac ctcaaccgtc gatatcgtta ccggtgcgga cgtgctggag
     3421 tactacgccg ggctgatccc gtcgctggaa ggcagccaga tcccgttgcg tgagacatca
     3481 tttgtctaca ctcgccgcga accgctgggc gtggtggcag ggattggcgc atggaactac
     3541 ccgatccaga ttgccctgtg gaaatccgcc ccggcgctgg cggcgggtaa tgcgatgatt
     3601 ttcaaaccga gcgaagtcac tccgcttacc gcgttaaagc tggctgaaat ttacagcgaa
     3661 gcgggcctgc cggacggtgt atttaacgtg ttgccgggcg tgggcgcgga gaccgggcaa
     3721 tatctgaccg agcatccggg cattgccaaa gtgtcgttta ccggcggcgt cgccagcggc
     3781 aaaaaagtga tggctaactc ggcagcctct tccctgaaag aagtgaccat ggaactgggc
     3841 ggtaaatcac cgctgatcgt tttcgacgat gcggatctcg atctcgccgc cgatatcgcc
     3901 atgatggcaa acttcttcag ctccggtcag gtgtgtacca atggcacccg cgtcttcgtt
     3961 ccggcgaaat gcaacgccgc atttgagcag aaaattctgg cgcgcgttga gcgcattcgc
     4021 gcgggcgacg ttttcgatcc gcaaactaac ttcggcccgc tggtcagctt cccgcatcgc
     4081 gataacgtgc tgcgctatat cgccaaaggc aaagaggaag gcgcgcgcgt actgtgcggc
     4141 ggcgatgtac tgaaaggcga tggcctcgat aacggcgcat gggttgcacc gaccgtgttc
     4201 accgattgca gcgacgagat gaccatcgtg cgtgaagaga tcttcgggcc ggtgatgtcc
     4261 attctgacct acgagtcgga agacgaagtc attcgccgcg ccaatgatac cgactacggc
     4321 ctggcggcgg gtatcgtgac ggcggacctg aaccgcgcgc atcgcgtcat tcatcagctg
     4381 gaagcgggta tttgctggat caacacctgg ggcgaatccc cggcagagat gcccgttggc
     4441 ggctacaaac actccggtat tggccgcgag aacggcgtga tgacgctcca gagttacacc
     4501 caggtgaagt ccatccaggt tgagatggct aaattccagt ccatattcta accaggaggt
     4561 ttatttgcaa tttgactaca tcattattgg tgccggctca gccggcaacg ttctcgctac
     4621 ccgtctgact gaagatccga atacctccgt gctgctgctt gaagcgggcg gcccggacta
     4681 tcgctttgac ttccgcaccc agatgcccgc tgccctggca ttcccgctac agggtaaacg
     4741 ctacaactgg gcttacgaga cggaacctga accgtttatg aataaccgtc gcatggagtg
     4801 cggacgcggt aaaggcctgg gtggatcgtc gctgatcaac ggcatgtgct atatccgtgg
     4861 caacgcgatg gatctcgaca actgggcaaa agaacccggt ctggagaact ggagttatct
     4921 cgattgcctg ccctactacc gcaaggccga gacccgcgac gtgggcgaga acgactacca
     4981 cggcggcgac ggcccggtga gcgtcaccac ctccaaaccc ggcgtcaatc cgctgtttga
     5041 agcgatgatt gaagcgggcg tgcaggcggg ctacccgcgc acggacgatc tcaacggtta
     5101 tcagcaggaa ggtttcggcc cgatggatcg caccgtcacg ccgcagggcc gccgcgccag
     5161 caccgcgcgc ggttatctcg atcaggccaa atcgcgccca aacctaacca ttcgtactca
     5221 cgccatgacc gatcacatta tttttgactg taaacgcgcg gtgggcgtcg agtggctgga
     5281 aggcgacagc accattccga cccgcgcgac ggcgaacaaa gaagtgctgt tatgtgcagg
     5341 cgcgattgcc tcaccgcaga tcctgcaacg ctctggcgtc ggcaacgctg aactgttggc
     5401 cgagtttgat attccgctgg tgcatgattt acccggcgtc ggtgaaaatc ttcaggatca
     5461 tctggagatg tatctgcaat atgagtgcaa agaaccggtt tccctctacc ctgccctgca
     5521 gtggtggaat cagccgaaaa tcggtgcgga gtggctgttt ggcggcaccg gcgttggtgc
     5581 cagcaaccac tttgaagcag gcggatttat tcgcagccga gaggaatttg cgtggccgaa
     5641 tattcagtat cacttcctgc cggtagcgat taactataac ggctcgaatg cagtgaaaga
     5701 gcacggcttc cagtgccacg tcggctcgat gcgctcgcca agccgtgggc atgtgcggat
     5761 taaatcccgc gacccgcacc agcatccggc aattctgttt aactacatgt cgcacgaaca
     5821 ggactggcag gaattccgcg acgcaattcg catcacccgc gagatcatgc atcaacccgc
     5881 gctggatcag tatcgtggcc gcgaaatcag ccccggcacg gaatgtcaga cggatgagca
     5941 gctcgatgag tttgtgcgta accacgccga aaccgccttc catccgtgcg gtacctgcaa
     6001 aatgggctac gacgagatgt ccgtggttga cggcgaaggc cgcgtgcatg ggctggaagg
     6061 cctgcgcgtg gtggatgcgt caattatgcc gcagattatc accgggaatt tgaacgccac
     6121 gacgattatg attggcgaga aaatggcgga tatgattcgc gggaaggaag cgttgccgag
     6181 gagcacggcg ggatattttg tggcaaatgg gatgccagta agagcgaaaa aaatgagtcg
     6241 tgatttgaac tgactcagga accgatcaga ttgagagtta ccgttccagg tatggggaca
     6301 gaatcgttat ataaaacata ggggtaactc tcatgcttca tagtgacaac ctcgccagca
     6361 aattctaagc tgactcgttc aatctcgctg atgaaggtac tatgaatgca ctgataaacc
     6421 gcaggtgaaa tgccactcag cttaagaatt acgccgctag tgttcctggc tatggtgact
     6481 tgttcactcc cgctcacggt tagcatatac gaaaagggtt cttgtaacga ggacaaaaat
     6541 aagtcagacc aaaacaacgt ggagctttaa acacaattcg tataatttat tagcagtgat
     6601 ttatttttac tcttctttaa ttaatcatca tgcttaaata ttttttagta caag
//
LOCUS       DAZKFZ010000236         6620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000236 DAZKFZ010000000
VERSION     DAZKFZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.236"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            317..1231
                     /gene="shuT"
                     /locus_tag="ACHOQW_004209"
     CDS             317..1231
                     /gene="shuT"
                     /locus_tag="ACHOQW_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFP9575173.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            1251..2588
                     /gene="hutW"
                     /locus_tag="ACHOQW_004210"
     CDS             1251..2588
                     /gene="hutW"
                     /locus_tag="ACHOQW_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFP9575174.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            2601..3095
                     /gene="hutX"
                     /locus_tag="ACHOQW_004211"
     CDS             2601..3095
                     /gene="hutX"
                     /locus_tag="ACHOQW_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFP9575175.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            3095..3718
                     /gene="chuY"
                     /locus_tag="ACHOQW_004212"
     CDS             3095..3718
                     /gene="chuY"
                     /locus_tag="ACHOQW_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFP9575176.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3767..4759
                     /gene="shuU"
                     /locus_tag="ACHOQW_004213"
                     /gene_synonym="chuU"
     CDS             3767..4759
                     /gene="shuU"
                     /locus_tag="ACHOQW_004213"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFP9575177.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCAIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4756..5526
                     /locus_tag="ACHOQW_004214"
     CDS             4756..5526
                     /locus_tag="ACHOQW_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFP9575178.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5578..6225)
                     /gene="yhiD"
                     /locus_tag="ACHOQW_004215"
     CDS             complement(5578..6225)
                     /gene="yhiD"
                     /locus_tag="ACHOQW_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9575179.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6289..6615)
                     /gene="hdeB"
                     /locus_tag="ACHOQW_004216"
     CDS             complement(6289..6615)
                     /gene="hdeB"
                     /locus_tag="ACHOQW_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP9575180.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
BASE COUNT         1654 a         1684 c         1614 g         1668 t
ORIGIN      
        1 ttatgaaacc tatcctgcgc cctgtaaacc accttgtttt tatggttatc cccccatgcc
       61 tcatcactaa attttgtcgt taacagtgat aattttttct tctctttgct ctgcaagcct
      121 cgatatctct gggttctcag ctttttcccg cgatgaaatt tagtcggaaa caagaacgtt
      181 agctgacgaa gcatcttgat aaaaatcaaa aataaatacg cttgtaacgc cttgcgtatt
      241 gaatcgacgg ttgtatttca tatataactc aaatgataat catgatcatt ctcaatttga
      301 taagagttct catattatgc caaggatcat caccaggccg tttctttttt ccccactgac
      361 gttatgtatt tcagccgtgg cctctgccgc gaagaccgca gtaaaacgta aaaaattgtt
      421 tacggcagta ctggctctta gctgggcttt tagcgtaacg gctgccgaac gtatcgtggt
      481 cgcaggagga tcgctgacgg agctaatcta cgcgatgggc gctggcgaac gcgtggttgg
      541 tgtcgatgaa acgacatctt atccaccaga aaccgccaaa ctgccgcata ttggctactg
      601 gaaacaactc agcagcgaag gcattttgtc acttcgcccg gatagcgtaa ttacctggca
      661 ggatgccggg ccacaaattg tgctcgacca actacgagcg cagaaagtca acgtcgtcac
      721 cctgccgcgt gtgcctgcca cccttcagca gatgtacgcc aacattcgcc aactggcaaa
      781 aacgctacag gttcctgaac aaggcgaggc gctggtgaca caaatcagcc aacgcctgga
      841 gcgagtacag caaaacgtgg cgaccaaaaa agccccggtt aaagccatgt ttattctctc
      901 cgcaggcgga agtgcgccac aagttgccgg taaaggcagc gtcgcggacg ccattctttc
      961 gcttgccgga gcagaaaacg tcgctaccca ccagcagtac aaaagctaca gtgcagaatc
     1021 actgattgcg gctaatccag aagtgattgt cgtaacctca caaatggtcg atggcgatat
     1081 taatcggcta cgttctattg ctggaattac ccacactgcc gcatggaaaa accagcgcat
     1141 tatcaccgtt gatcaaaacc tgattctggg aatgggtccg cgtattgctg atgtcgttga
     1201 gtctttacac cagcagcttt ggccgcaata aatggattta aatcagacga atgaacgcaa
     1261 ataacaccct ggatctcacg ccacattttg ctttagacgg tgatcagcca tttaaagaca
     1321 ggcgcgccat gatgccgttt cgtggagcca ttccggttgc caaagagcaa ctggcacaga
     1381 cctggcaaga gatgatcaat caaacggcat cacctcgtaa gcgactggtt tacctacaca
     1441 ttcctttttg tgcgacacac tgcacgtttt gtggttttta tcagaaccgt tttaatgaag
     1501 atgcgtgtgc tcattatacc gacgccctga ttcgtgaaat agaaatggaa gcggacagcg
     1561 tattgcatca gtctgccccc atccacgcgg tctatttcgg tggcggtacg ccttccgcac
     1621 tttcggcaca cgatttggcc agaatcatca ccacattacg agaaaagctg ccactggccc
     1681 ctgattgtga aatcactatc gaaggacggg tattgaattt tgacgctgag cgaatcgatg
     1741 cttgccttga tgcaggagca aaccgcttct caattggtat ccagtcgttt aacagcaaaa
     1801 tccgcaagaa aatggcccgc acctcggatg gcccaaccgc tattgcgttt atggaaagcc
     1861 tggtcaaacg tgaccgtgct gcggtggtct gtgacctgct gtttggcctg ccgggtcagg
     1921 atgcgcaaac ctggggagaa gatctggcta ttgcccgcga tatcggtctc gacggcgtcg
     1981 atctctatgc gctcaatgtc ctgtccaata caccgctggg caaggccgtg gaaaatgggc
     2041 gtactaccgt accctcaccg gcagaacgtc gcgatcttta tctgcaagga tgtgatttta
     2101 tggacgatgc cggttggcgc tgcatcagta acagccactg gggccgtacc acacgcgaac
     2161 gcaatctcta taacctgctg attaaacaag gtgccgattg tctggccttt ggttccggag
     2221 ccggcggttc gattaatggt tactcctgga tgaacgaacg caatcttcag acctggcatg
     2281 aatccgtcgc ggcaggcaaa aaaccgctga tgttgatcat gcgtaacgcc gaacgtaatg
     2341 cgcaatggcg tcataccttg cagtcaggtg ttgaaacagc gcgcgtaccg ctggatgaac
     2401 ttacgccaca tgccgaaaaa ctcgcgccgt tactggctca atggcaccaa aaaggcttaa
     2461 gtcgcgatgc ttcaacttgc ctgcggctga ctaatgaagg tcgtttctgg gcaagcaata
     2521 ttttgcagtc tcttaatgaa ctaattcagg tacttaatgc gccagcgatt atgcgtgaaa
     2581 aaccataaca ggagtgattt atgagccatg tctcgttaca agaatttttg aaaacggagc
     2641 cggacggtac gctggaggtg gtagccgaac aatacaacac cacactgctg gaggtagtga
     2701 gaaatctacc ctcctctacc gtagtgccag gcgataaatt cgatactgtc tgggataccg
     2761 tctgcgaatg gggcaacgtc accacgttag tacatactgc cgatgtaatc ctcgaattta
     2821 gcggcgaact gccttcgggt ttccatcgcc acggctattt caacctacgc ggaaagcacg
     2881 gcatgtccgg gcatatcaaa gcagaaaact gcacgcatat tgccttaatc gaacgtaaat
     2941 ttatgggcat ggacaccgcg tccattctct ttttcaacaa agaaggcagc gccatgctga
     3001 aaatcttcct cggcagggat gaccatcgtc agctcctgag cgaacaagtc agcgccttcc
     3061 atactctggc ggcatcgtta aaggaacacg cctgatgacg ccgtggctac tctttggcgc
     3121 aggcggaaaa ggtgtgggtg ccagaacgct tgaactggcg ctggcggaac aacgtccggt
     3181 cgttgctgtc attcgtcatg ccgatgccgc cacgaaactg gcgcaacaag gcgtacaggt
     3241 ttttacaggc gacgcctgtg atgccagcgt agtcgcggct gcttgccgcg cagctggccc
     3301 ggatgcgctt atcatctcaa caatgggagg agctcaggat tatctggcgc acagaacagt
     3361 gattgatgag gccgaaaagg ccggcatcag tcgcatgatt ctggtgacct ctttgggatg
     3421 cggcgatagc tggccgtttt tatcggagcg ggcaaaggct gcgtttggtc aggcggtacg
     3481 ggaaaaaacg ctggcggaaa gctggctgca aaccagccag ctagactacg ccattctccg
     3541 ccctggtggt ctgcttgacg gcgcagcaac aggcaaagca caacggatac aaaatcagga
     3601 gtgtcatggt tttgtccgcc gtgccgatgt cgccgcacat atccatgaac tggcaaatgc
     3661 gccagcactt aaccagcagg tctacagtct gattgaaccg gacctgaaac cggcgtgata
     3721 ccccggcgcg gcatgtccgc gccgtaatgt cgtaacggag ttttgcgtgc tcaaggattc
     3781 attttcatcc gccagggtct ttatggggct atcactatta ttgcttgccc tggtgctgtt
     3841 cggtgctagt cagggagcgt taaagatcag ttttgatgcc ctttttgatg aggaataccg
     3901 cgatatctgg ctcaatattc gtctaccacg ggttttgctg gcggtgctgg taggtgcagc
     3961 gttggcaacc gcaggggtaa ttatgcaggg gctttttcgc aacccaatgg ctgacccagg
     4021 attactcggc gtcagtagcg gttctgcatt aatggttggc gttgcaattg tgctgccctt
     4081 ctccttccca gtggtgctgg tgctctatga gcaaatggtg tttgccattg ccggaagttt
     4141 agtagtctgc gccatcattt ttctcatcac gcagcgccat cgcgatggca gcatgatgca
     4201 attattactc gccggtatcg ccatcaatgc cctgtgcggc gcagcgatcg gcatcctgag
     4261 ctatattggc gatgagcagc agctacgaca actcacattg tggatgatgg gcaatcttgg
     4321 acaggcgcaa tggccgacgt tattggttgc cagttcattc atccttccgg ccattatcgc
     4381 aacaacttgt ctcgccggaa cgctgaattt actgcagctc ggtgatgaag aagcccatta
     4441 cctcggcgtg aacgttaagc gtaaacgcca gcaattacta ttagtgagct cactgctcgt
     4501 tggtgctgcc gtatcggtaa gcggcattat cggctttatt ggcctggtga tcccgcatct
     4561 gattcgcatg actaccggag caaatcaccg ctggctcatc ccttgttccg ccctcgccgg
     4621 agcctgttta ttgctgatgg cagacacgct tgcccgcacg ctggtacagc cagcagaaat
     4681 gcccgtggga ttattaacca gcctgcttgg tggcccttat tttatgtggt tgattctgcg
     4741 aaatcggagg atcacatgat cagcgcccag aatctggttt atagccttca ggggcggcgt
     4801 ctgactgata atgtctcgct gacttttccc ggtggggaaa ttgttgccat tctcggccct
     4861 aatggtgcgg gaaaatctac gctgcttcgc cagttaaccg gctatcttca acctgatagc
     4921 ggcgaatgtc ggttgtttaa taagccatta aatgaatggt cgattactga actggcgaaa
     4981 caccgggccg tgatgcgtca aaatagccac atggcgtttc cattcagcgt acaagaagtt
     5041 atccagatgg gtcggcatcc gcatcgtact ggtaatcagg ataatgaaac agcacaaata
     5101 atggcgttat gtgactgtca ggcgttagca aaccgcgact atcggcaatt atccggtggt
     5161 gaacaacagc gcgtgcaatt agcgcgcctg ctggtgcaac tctgggaacc aactccctca
     5221 ccgaaatggt tatttcttga tgaaccaact tcggcgctgg atattcacca tcagcagcac
     5281 ttgtttcgtt tattacgcca gttagttcac gagcggcagt tcaatgtctg ctgcatattg
     5341 cacgatctaa atcttgccgc gcattatgca gaccgtgttg tattaatgca aaaaggaaag
     5401 gttatcgcaa atggtaaacc acaggacgta ttgacacagc aggcgctgac aatgctctac
     5461 ggcgcggata ttaccgtact taaagatcct gccaaccatt caccactgat cgtcctcgat
     5521 cattaaaagc aacaggagta ccgcactggt actcctgcga ataaataact atacagatta
     5581 gctaattgaa acgcctttca cccctgccat accttttaat aatcgcaaca gatcttctat
     5641 cgacgttgtg gcatgaagct ggatatcaat ggcgaccact tcatgatctt cattttcctg
     5701 caacgaaact aaatcagttt ttattttttg ctgcttaaac cagtcgagca ttgataccgt
     5761 attaccgttc actaatgtca gctgtaaatg gtaatttttg ttcatcaggc ggaaggttaa
     5821 ttgatggaag acttccagca ccagcaaggt catcactgaa ccataaatac ccagttcgta
     5881 cataccactg ccaataacca tacctatggc ggcggtcacc cagatatccg ctgccgtcgt
     5941 caaacctaca atattttggt tacgaacgag aatgttacct gcaccgataa acccgacgcc
     6001 cgtcaccacc tgagcagcaa tacggctggg gtccagtccg acgtgatcta aagacagcac
     6061 gtcagcaaaa ccatatttcg aaacaatcat aaacagtgcg cttcccatgc caattaatac
     6121 atgagtgcgt aaccctgctc ctttgccgcg catttgcctt tccatgccaa tagcgccaca
     6181 ggcaattgcc gcgagtataa gacgtataat aaattctgct gtcatataaa aatgcaaaaa
     6241 ggagcagcaa gatggctcaa cttgccactc ctttttactt gtacctcatt aattcggcaa
     6301 gtcattagat gcttgatttt tgaaggtata caaatttttc tgcgggtttt tcttacagta
     6361 ttcgatcact ttaggaattt gagtgagatc ggtttcattt aaagtaactg tatcgccacc
     6421 tttatatact gtttcttcat gcagcatcca ccatgcaacc ggggtcattg cttttggatt
     6481 cagatcaata aattcctggc aggtcatatc tttagcggat tcattggctg ccagcgcaga
     6541 ttgtgcgttc accagtgaca aagccgctac agcgcccata aaaataaacg ctttacggag
     6601 agatgaaata tttattttgt
//
LOCUS       DAZKFZ010000237         6530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000237 DAZKFZ010000000
VERSION     DAZKFZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.237"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(119..571)
                     /gene="yafP"
                     /locus_tag="ACHOQW_004217"
     CDS             complement(119..571)
                     /gene="yafP"
                     /locus_tag="ACHOQW_004217"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9575181.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(581..979)
                     /gene="yafO"
                     /locus_tag="ACHOQW_004218"
     CDS             complement(581..979)
                     /gene="yafO"
                     /locus_tag="ACHOQW_004218"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFP9575182.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(982..1275)
                     /gene="yafN"
                     /locus_tag="ACHOQW_004219"
     CDS             complement(982..1275)
                     /gene="yafN"
                     /locus_tag="ACHOQW_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFP9575183.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(1327..2382)
                     /gene="dinB"
                     /locus_tag="ACHOQW_004220"
     CDS             complement(1327..2382)
                     /gene="dinB"
                     /locus_tag="ACHOQW_004220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9575184.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2453..3238)
                     /gene="lafU"
                     /locus_tag="ACHOQW_004221"
     CDS             complement(2453..3238)
                     /gene="lafU"
                     /locus_tag="ACHOQW_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP9575185.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3210..4922
                     /locus_tag="ACHOQW_004222"
                     /pseudo
     CDS             3210..4922
                     /locus_tag="ACHOQW_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(5067..5564)
                     /gene="rayT"
                     /locus_tag="ACHOQW_004223"
                     /pseudo
     CDS             complement(5067..5564)
                     /gene="rayT"
                     /locus_tag="ACHOQW_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(5740..6459)
                     /gene="yafL"
                     /locus_tag="ACHOQW_004224"
     CDS             complement(5740..6459)
                     /gene="yafL"
                     /locus_tag="ACHOQW_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9575186.1"
                     /translation="MSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQT
                     HLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSS
                     DHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEM
                     YHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLA
                     EPFWQDHFLGARRILTEETIL"
BASE COUNT         1553 a         1692 c         1686 g         1599 t
ORIGIN      
        1 tggaaaggtc tgcaaagagt cattgataaa ggatttgtca tgatttacct ccttgcagtt
       61 tgtggaataa gaaaacgcgc acataccagg cgggcgttat tttcattgca agctggattt
      121 aatgttgcgg tttatatcgc atagaaaaat tagtaaacca cgctccccgg cattcaacgc
      181 gctgctgctt aactgtctga aaaccataac gttcaaaaaa gggttttgcg gttatgctgg
      241 cgtccaccgt aagttcggat tcagacttaa tccaaggttt taacaaagcg ctggcaaccc
      301 cacggcgggt gtattcaggg tcaacaaata acatatcgat ataatgttca atgcgggaaa
      361 taaaaccaac cggttgtgca ttaatgactg caacccgcac ttgtgatttc gcgagtttct
      421 ccttccagcg agattcgtca atctgcgccc aggcggcaat ttgttgtggt gaataatgct
      481 gactggcggt catcataacc gctctaatga aaatagcgca tagttgctga aaatcgccag
      541 gctgatagtt tcttatttgt atgttattca taatataaat tcaaaaacgc atgcgaaacg
      601 cttctgccat tttcccaatt ttatgcattt ggttgttatc tcgagccagt ttatgaggtt
      661 caggtttcag aatggcaatg agcaaccatg cttgctcatc aaacgcaccc tgacaatata
      721 ccaaatgcga ttcgtcattc gttctgctga attggcgcaa ctgtggcgga aatggattat
      781 tcacatttgc cagatgaata tgagcaactc gctcaaattt gattaatggc caagtaaacg
      841 agtcgtcgta gagtgcatcg cgaccaaata tatctggcaa aacaccgtca cgcttatagg
      901 aaataaaatc cgccgttaac gcatcaagct cctctgctgt aagttgcagg cgaataagtt
      961 ttgttttgaa tacccgcatc cttattcctt aaagtcattg aaatcatcat ccgtcatatc
     1021 attaagatat ttttcagctc ggcgtgtaat ttcctctaat cttgcagcac ttggacggaa
     1081 gcgcgccttt atgggctttt tctcctcttg ttcagcaagc atgtccatta acgcttcgaa
     1141 tgcgctggca cttaagagat atcctgcggg gcgattatta gaaagaaccg caaccggttg
     1201 atcaataaag tatttagctg ggtttttacg taattcagtg atattgaccg atttttcagc
     1261 gagaattcga tgcatacagt gatcccctca taataatgca caccagaata tacataatag
     1321 tatacatcat aatcccagca ccagttgtct ttccatttga gggtcaagca acgtcacatg
     1381 cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt tacgcgcggt
     1441 ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgggtgg tttgctgaaa
     1501 atcgtcgaac tttaatttca ccccctggcg agcaatcagt aaatcaggtt ttacctttgc
     1561 cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt cagaccagtg
     1621 atgaatatct tccgccatcg tgcgttccac gccgacggat tttcgtaacc gttcgctgtt
     1681 aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa atttgccaaa
     1741 gcgtttaagc agaatcacca gatcacactt ttgtacatca ccgcaggtcc gcagccccat
     1801 cgcttccagc tttgccgctg agactttgcc aacaccggga atttttgcca gcggtaaggt
     1861 ttgcagaaat gccgaaactt ctgccggcgt aatcacaaac tggccgttgg gtttattcat
     1921 gtcggaggcg attttggcga gaaactttac tggtgccacg cccgcagacg ccgtcagttg
     1981 cagctcgctg aaaattgtct ggcggatttc ctgggcgatg agggtcgcag aaccgtggca
     2041 atggacgcta tcagtgacat cgaggtaggc ttcatccagt gacaacggtt caatacgcga
     2101 ggtgtagcgc gagaagattt cacggatatg atttgaggct tctttgtagg cgtcaaagcg
     2161 ccccggaagc aaggtgagat gtgggcataa tttgagcgcc atccctgtcg gcatagcgct
     2221 gcgtacgcca aatttacgcg cgggataatt ggcggtgctg atcacccccc gacgttcgcg
     2281 gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca tctccactgc
     2341 ggcgaaaaag cagtccatat ccacatgaat gattttacgc attactcacc tctcaacact
     2401 ggtaaagtat acagtgattt cagggtttga gaaatgcgta aagattcagc atttagcgcg
     2461 ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc accactttat
     2521 ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc agcaccataa
     2581 tctcaatgcg tcggttgccc gcgctttgcg gatttttcgc gtccagcagc atctggtccg
     2641 ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc agcacccgac
     2701 gagctgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg tttttgtagg
     2761 ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg aacactggcg
     2821 cgagctccac cagcaaagtt ttaaagaacg gcataatctg cgcgctgccg cgttcgaaca
     2881 tattgcgatt ctggtcgtct ttaatcagca cgcgtaatcc ctgcgggaca atctccatct
     2941 ccagattcgc ttccatatgc gcatcacggg caatggtgtt gatactggtt gccagctcgc
     3001 caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattcaca tcacgcgctt
     3061 ttttctccgt ctcttccggc accgccacgg tggcaggttt tggcggatga gacgggctga
     3121 ttttatttaa cggcgacagc ccaccaccgt taaaaatcga ctgcccgtgc agcgcggcga
     3181 taatgctttc acgctcggat ttactgacgc tgttgacaat caactttatc gtcgtcacca
     3241 aaggggccga gcgtatttcc gaggtttctg cccgctttac cctggatgcg atgcccggca
     3301 aacagatggc gattgacgcc gatcttaacg ccggattgat caaccaggca caggcgcaaa
     3361 cccggcgtaa agatgttgcc agcgaggccg atttctacgg cgcgatggac ggggcatcga
     3421 agtttgtgcg cggggatgcc atcgccggga tgatgattct ggcgattaac ctgatcggcg
     3481 gcgtctgtat cgggatattt aaatacaacc tgagcgccga cgccgccttc cagcaatatg
     3541 tgctgatgac catcggcgat gggctggtgg cgcagatccc ttccctcctg ctctccaccg
     3601 cagcggcgat tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat gacgtgcgcc
     3661 accagctgct ggcaagcccg tcggtgctct acaccgccac cgggattatg tttgtgctgg
     3721 cggtggtgcc gggaatgccg cacctgccgt ttttgctgtt cagcgccctg ctgggtttta
     3781 ccggctggcg gatgagcaaa cagccgcagg cggcggaggc ggaagagaaa agcctcgaaa
     3841 cgctgacccg caccatcact gaaaccagcg agcaacaggt cagttgggaa accattccgc
     3901 ttatcgagcc tatcagttta agcctcggct acaagctggt ggcgctggtc gataaagcgc
     3961 agggcaaccc gctcacccag cgtattcgcg gcgtacggca ggtgatatcc gacggtaacg
     4021 gcgtgctgct gccggagatc cgcattcggg aaaacttccg cctcaagccc agccagtacg
     4081 ccattttcat caacggcatt aaggccgatg aagcggatat tccggcggat aaactgatgg
     4141 cgctgccctc cagcgaaacc tacggcgaga ttgacggcgt gctggggaac gacccggcgt
     4201 acgggatgcc ggtgacctgg atccagcccg cgcagaaggc gaaggcgctg aatatggggt
     4261 atcaggtgat cgacagcgcc agcgtgattg ccacgcatgt gaacaagatt gtgcgcagct
     4321 atattcctga tttgtttaat tatgatgaca tcacgcagct gcataaccgc ctgtcgtcga
     4381 tggccccgcg cctggcggaa gatttgagcg cggcgctgaa ttacagccag ttgctgaaag
     4441 tgtaccgtgc gctgctgacc gaaggcgttt ccctgcgcga tatcgtcacc atcgccaccg
     4501 tgctggtcgc cagtagcgcg gtgactaaag atcatattct gctggcggcc gatgtgcgcc
     4561 tggcgctgcg gcgcagcatt acccatccgt tcgttcgcaa gcaggagctg acggtgtata
     4621 cgctgaataa tgagctggaa aatctgctga ctaacgtagt gaatcaggcg caacagggcg
     4681 gtaaagtgat gctcgacagc gtgccagtgg acccgaatat gctcaaccag ttccagagca
     4741 cgatgccgca ggtgaaagag cagatgaaag cggcggggaa agacccggtg ctgctggtgc
     4801 cgccgcagct gcgccctttg ctggcgcgct atgcaaggtt gtttgcgccg gggctgcatg
     4861 tgctgtcgta taacgaagtg ccggatgagc tggagttgaa gataatgggg gcgttgagtt
     4921 gattggcctt taaggggcgc atccggcatt ggtgcacaat tgccggatgc ggcgtgaacg
     4981 ccttatccgg cctacggttg agtgcagttt tgtaggcatg ataagacgcg ccagcgtcgc
     5041 atcaggcatc cggcaccagc gcacaattat gaaatgatac gctccccggc actaaaatcc
     5101 gttatgtctc ccgcccaatc aatgggatat aaccctcgcg ccacatcgcg atggaacgtt
     5161 gagaatggcc aatcactcac ttgctttacc cagccatgtt ttactggatt tatataaata
     5221 taatcaacat gatggcgata gtctttggta ttgcggatgg tgtgctccta aaaacgtggt
     5281 tgccagatat ttttcaatcc acaagcatgg gtgaactgct ttttaatctc ccgccagcgg
     5341 gaggaaaaat catcatcgct ttcgggtaat gtccatatgc aatgcatatg ctccggcaaa
     5401 acgacccagg cgttaatttc aaaaggtctg tctcgcttaa cgttaataat ggcgttacgg
     5461 agcatctgga actgagcggt cagaaggtgg cttcgacgat ttcgtaggtt aaccgtgaaa
     5521 aaccaggttc cccctttgat gtaataccga cgatattcag acatggcaac tcctttttct
     5581 caacggtaag aagaaaagga aaggtcggca atgaggtgat ggaataacgg gaaagcggct
     5641 cgcaaaaacg cacagatttg taggcatgat aagacgcgtc agcgtcgtat caggcatctg
     5701 cgcacaactg ccggatgcgg cgtgaatgcc ttatccgtcc tacaaaatcg tctcttccgt
     5761 caaaatccgg cgcgcgccta aaaagtgatc ctgccagaaa ggttcagcta accgacttac
     5821 ccgaatggtt tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc
     5881 catatggtcg gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag
     5941 gtcgttattc gccacaatcg ttgcccgatg gtagtgatac atctcattag ccgtacgcgg
     6001 gagcttcgcc tcgaggatct tgttgtaggc ataaaaaacc aacccgctac agtcaaagcc
     6061 tttatcaggc cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccaggcggtg
     6121 aatcgccact tcggtaatgt tatgcagatg gtcgctgctt aaaaagtggt tgttttccgc
     6181 cagcgcctgc cagcgtctgt cactcgcctt gagcggtgca ggaaaccatt ctggatgctg
     6241 ttttatctgt tcgcggttgt gctggcgtag cgcccttctg ctttcggcat tgccttccac
     6301 aatatagctg acctgctttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt
     6361 ttgcatccgc tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgctggcaaa
     6421 tgaagaaaac ggcaaacaaa tcaacagtaa tcccgacaat acaaaggatg gaatcgacgg
     6481 caaggacata ctcaataatg gctggtcatg cattgatgtt acagtgtatc
//
LOCUS       DAZKFZ010000238         6522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000238 DAZKFZ010000000
VERSION     DAZKFZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..409
                     /locus_tag="ACHOQW_004225"
     CDS             <1..409
                     /locus_tag="ACHOQW_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9575187.1"
                     /translation="TLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTW
                     DRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHE
                     LDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(417..683)
                     /locus_tag="ACHOQW_004226"
                     /pseudo
     CDS             complement(417..683)
                     /locus_tag="ACHOQW_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            841..2025
                     /locus_tag="ACHOQW_004227"
     CDS             841..2025
                     /locus_tag="ACHOQW_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP9575188.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2025..2537
                     /locus_tag="ACHOQW_004228"
     CDS             2025..2537
                     /locus_tag="ACHOQW_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP9575189.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2592..2957
                     /locus_tag="ACHOQW_004229"
     CDS             2592..2957
                     /locus_tag="ACHOQW_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575190.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            2966..3121
                     /locus_tag="ACHOQW_004230"
     CDS             2966..3121
                     /locus_tag="ACHOQW_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP9575191.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3108..5911
                     /locus_tag="ACHOQW_004231"
                     /pseudo
     CDS             3108..5911
                     /locus_tag="ACHOQW_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5924..6412
                     /locus_tag="ACHOQW_004232"
     CDS             5924..6412
                     /locus_tag="ACHOQW_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575192.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
BASE COUNT         1561 a         1580 c         1893 g         1488 t
ORIGIN      
        1 cacacttgta gaccgaaaat cacgttatac gatcatcctc agactcaggg gcaaagattc
       61 tgtctcagta aatcaggctc ttaccgacaa attcctgagt ttaccgtcag aactcagaaa
      121 atcactgaca tgggacagag gaatggaact ggccagacat ctagaattta ctgtcagcac
      181 cggcgttaaa gtttacttct gcgatcctca gagtccttgg cagcggggaa caaatgagaa
      241 cacaaatggg ctaattcggc agtactttcc taaaaagaca tgtcttgccc aatatactca
      301 acatgaacta gatctggttg ctgctcagct aaacaacaga ccgagaaaga cactgaagtt
      361 caaaacaccg aaagagataa ttgaaagggg tgttgcattg acagattgaa tctacaagtg
      421 cctttttctg tcagcggttc tcctttccag ccaatgagaa ccgtcaggcg cgcgccccgt
      481 ggcggtagct gcaactgacc atctgcatca tccagcgtga tggtgagctg gtccgcctca
      541 aatccccggt tgtcggtcag tgacaggctc atcaggcgct ctgccacgcc tgacagcgtt
      601 ttaccctccg cgagaatatc aaaatccggc actttcacgt ggtctgtgcc ctgactgagc
      661 aattgcatgg tggtgtcggt catctgttcc ctccctgtgc ggcatggtcg catgtgcgtg
      721 cggagggggt tactgctttt tgttgtcgcc gtggcgggag aacggcgcag gggtgagatt
      781 acgcgcgtgg tgggtgatga ttgttgccga atcatttaac ggatacaagg ggctgaagct
      841 atgagtgaaa ctcgttttca tggtgcccgt gttacggaaa gtaccgacct ggtaacagcg
      901 attaatgatg ttgattccag tgttatcggt atcgtggcaa cggcagatga tgcggacgcg
      961 gagctgttcc cgctgaacaa gcccacactg ctgacccgcg tcaatgacgt gctgggaaaa
     1021 tgcgggacta cggggacgct ttatcgtgcg cttaaggcta tcgcagacca ggtgagcaca
     1081 aaagtgatcg tcgttcgcgt ggctgaacac aaagaagaag gcggtaagac gcaggatcaa
     1141 ctggttatcg gtggttctga atctgacggc agctatacgg ggatgtatgc gctgcttgtt
     1201 gcagagcagg atgaaagcat cggataccgt ccgcgtattc tggccgcgcc ggagctggac
     1261 acggaggcgg taacaaaatc cctgtgcgtg attgcgggta aactgcgcgc gtttgtgtat
     1321 gccacatgtc atggttgtaa cacgatggct gaggcgatta cctaccgcca gaaattcaac
     1381 gaacgtgagg tgatgctctt atggcctgac ttcatcgcct acaacctgaa aagtggcaaa
     1441 aacgaaacgt tccccgcgcc tgcttatgcg tgcggccttc gtgcgtacat tgaccatgag
     1501 cagggctggc acaaatcgct gtccaacgtt ccggttaaaa atgtgcttgg gatgtcgagg
     1561 catgtgttct ggtcgttgca ggccgaagac agtgatgcca acagcctcaa caacaaagaa
     1621 atcacgacca ttattcgtcg caacgggttc cgcttctggg gcaaccgcac accggaaacg
     1681 aacgcctaca tctttgaggt gtatacccga accgcacagg tgctggctga ttcaattgcg
     1741 gaagcgcagt ttgaaaccat cgacagtcca ctgacacctg cgaacgtgaa ggatgttatc
     1801 agtgccatca gggcaaaact ggattcactg gttactgccg ggaaactgat tggcgcggag
     1861 tgctggtatg acatcgagga taacagcacc acgaatttac gtcaggggcg tgtgcgtatt
     1921 cgctacaaat atacgcccgt tcctccgctg gaagacatgg agctttacca gtcgtttact
     1981 gatgaattct ttggtcccgc atttgcggtg ctgggaggtg cctgatggct gtaccaaaac
     2041 atcttcgctt ttttacgctg tttgtggatg gtgaaaacga agtgggtaag gtgacgtccg
     2101 tcaccctgcc taaactgacg cgcaaaaccg acagctaccg gggtggtggc atgatgggtg
     2161 cggtaagtat tgacctcggc ctggacgact ccgcgcttga tgcgagcttt gtcatggggg
     2221 gcgcagttcg tgagctgttc ctgaagtatg gcggcacgat tgacggcacg ctactgcgtt
     2281 ttgcggggga atactacacc gatgatgaaa gcgacctgta tgaagtcgaa atgcgcggac
     2341 gtgtgacgga aattgatatg ggggaagcca aacagggcga agccacatca cacacttacg
     2401 ccattaaaaa cacctactac aagctgagtg ttaacgatcg cccgttgtgg gagattgacc
     2461 tgctgaactt catttaccgg aaggacggca aggacattgt gcccgatcgc atccgttccg
     2521 cgcttgggct tggctgataa gtaatatgca ggcggcgcag tgcgtcgcct ctgactgaaa
     2581 ggagtttcct gatgaaagag acgaaaaaca tcgataccga aaacacggta gttactgaca
     2641 ctgtgaaaga aaccagtgag cgtggtgtaa aacttaccca accaattgag cgaggcggcg
     2701 aaaaaatcac gtatgtggag atcaccgggg ctattgagca ggctggatct ctgcgagatt
     2761 tgtcgctgtc tgatgtgctg aatctgaaag cggaatccat gtttacgctg ctgtcacgcg
     2821 tgacatcacc gcgactggat gaagtgacga tcaaaaaaat ggcatcccgt gactttattc
     2881 agttatgtgt ggttgccgta aattttttga gcggtgcgga ctctggcggg aagaacgaac
     2941 aggcgacgga agcctgatca cggttgtgtg ctttgagcac atagaagact ttgtggcaga
     3001 tattgccgtt atttttaact ggtcgcccgc cgaaatcttc atgatgacgc ccggcgaagt
     3061 ggttagctgg cgtgagcggg cggcacttcg cagcgggaat gcagacaatg aagactcttg
     3121 atatccgggt cgctttcagc gccgttgaca ggctgacccg acctgccgaa aacgcacgcc
     3181 gcctgatggg gcagtttggt gactccatcc agcgaacgca gggggcgatc aaaaatctcg
     3241 agcgtcaggc gcgatcattt gagcgcgccc gcgacgctgt cagtaaagcg gatgctggca
     3301 tcgtgaaagc acgacgccag cttaacgccc ttaatcagtt acaacgcacg ggtacagtgc
     3361 tcagcgaaaa acaacaaaag ctgatgcagc agttaagcac ccggcttgaa cgcctgaatg
     3421 aatcgcgcac acgggaaatt cagaaaatga gggaacttgg cggagagctg aaacgccacg
     3481 gcatttccct gacaggcagc gataacacca ttcagcaggc catcagacgc accgaacaat
     3541 acaacaacca gcttgaacgc gaacggcagg cgcttgcgcg tgtaacgcag gcgcgtgagc
     3601 ggtattcgca cgcgcaggaa accgcgggaa aactgaaaac aggtggtgcg ctggcaattg
     3661 gtgcggcagc ggcgggcggc tatgctgccg ggcgtttttt gcagcctgcg atcgggttcg
     3721 gcaaagagat gtcccgcgtt caggcactga cgcgaatcga caaaaacagc ccgcagttta
     3781 aggcgctgcg tgagcaggcg ttaaaacttg ctgaaacaca gtttactgcg agtgatgccg
     3841 ccagtgggca gagctttctg gcaatggctg gttttactcc gcaggccatt caggccgcat
     3901 tgcccggtgt tcttaatatg gcgctggcag gtggcgtcga actcggcgag acggctgata
     3961 taggctccaa tatcctcaca cagttcaacc tgacagccga tcaaatggac cgggtgggcg
     4021 atacgctgac agcagcattt acccggacca atactgattt acgcgcgctg ggcgaaacca
     4081 tgaagtatac cggtccggtt gccgcaaaac ttggtatcag tcttgaagaa gcggcggcca
     4141 tggccgggat gcttgccaat aatggtcttc gcggaagcga tgctggcacg gccatgcgcg
     4201 caagtctgtc ccgccttgca tcaccgccaa aagctgcggc tgatgcactg aaagagctgg
     4261 gggtgtcagt tgctgacgcc agaggcaaaa tgcgcccgat ggaggatgtg ctgcttgatc
     4321 tctataaggc gacacaaaaa tacggacagg tggaccaggt ctccttcttc aaggacatcg
     4381 ccggagaaga ggcgttcgtt ggtttgcaga cgcttgttgc ggcggctggt tcaggagagc
     4441 tgcaaaaact gaccagagaa ttgcaggggg caaggggaga ggccgatcgc gtcgcaaaag
     4501 taatggctga taatcttgat ggggacctga aaaatctcga cagcgcatgg gaaggtcttc
     4561 gtattcgcat cagtgatctg gttgacggtc cgctgcgttc tgtcacgcag tggctcacgc
     4621 gggtgcttga aaaaatcacc tcgctggcgc aggcccatcc ggtactgacg cgccagctac
     4681 tgatagcagg cggtgcgttg ctggcaatga ctgcaacgat tggctcgttg tcgctggtta
     4741 ttggggtgct ttacgggaag ctggccaccc tgcgtcttgg ttttgacatt cttacccggt
     4801 caatgaatgt cgtcagggtg ttacctgcgc tatggggaat ggtgacgggt tccgtttctt
     4861 tactgggagg cgttatcggg gcgttgttca gtccggttgg tcttatcgtg gctgcgcttg
     4921 ccggagctgc cgttcttatc tggaaatact gggatcctat cagggctttc tttgccgggg
     4981 tgttcagcgg gattatggaa aggctgaccc cgttgcgcga cacctttgaa cggtttggcc
     5041 ctgtttttga cgtaatcgga agcggttcag ccaggtgttt aactggttta aatcgctgct
     5101 gtcaccgatg gagtccagca aggaaacgct ggataaatgt accagtgctg gcgaggtatt
     5161 cggtaacgtt cttggtggtg cgttacagct tgttctgacg cctgcaaaaa tgttgctgga
     5221 tacgctggcg tggatacttg aaaaactcgg tgtgcttccg gatgaagcgg aaagggcgcg
     5281 caagaaaatc gaagacgcac aacgtgcggc cattcttcag gacaaggttg ctctgtttca
     5341 gggagacatt gcgaaaatca atccgccgaa gtctgcggga aatagcaatg gcaccggaga
     5401 tgataaaccc aaagacaaca aacctctcac agacagcaat accggcacgc tacgcagact
     5461 cagcaaaatt gctgataaca caggtaagct ggttgatgag acaaaaaaac gcattggccc
     5521 cggcgatatt gtctttaaga acctgccccg cgcacttgct gttcgtgggg agtggcagga
     5581 gcggaagatt gcgcaggtca gtaagcctgc ccccgcaatt aatatcacac ccgtggtccc
     5641 ggctccgctg cctccggcgc tggtccctgt tgttgcggcc agctcccgcc cggtggtgga
     5701 ggccatacga tcgccagtgg catcagttcc tgcaacttcc cgtaaccggg agcctgttgc
     5761 ctccggattt ggcggtgaaa ttcatgttca tctgcataac gttgttacgc agaatccccg
     5821 cgaactggcg aaactggtcg gcgaaatggt cagggcagaa atggaacggc gcgcccgtgc
     5881 caggcgtggc agtttttacg ataaagattg aggagtcatg gccatgatga tgatctacgg
     5941 catgtttgtt tttgagctgc gcacgttgcc gcatcagcag ttacagcaaa acaaaagctg
     6001 gcgacatgtg aaaaatgaac gcgttaaccg ttcagcaagc tggcagtata tcggtgcagg
     6061 tgatgatcgc atcgttcttt ctggtgttct ttatcctgag attactggtg gcgaagtgtc
     6121 gctgtcgttg ttgaccacgc aggcatatac aggacgtcct tggcctttga ttgatggcgt
     6181 cgggcagatt tacggcatgt atgtcctgac cggaacgaat acgacccgct ccgagtttga
     6241 tcgctacggt aaggcgaaaa agatagaatt ttcactgact cttgaacgct gtgatgagga
     6301 tttgcgggag cgcctgcaat cctcatcgtt cagcgatatg ctgtccggct tcaaagataa
     6361 ggtgacatca tcccttaata gcgcggccag ctcagttaag gggctgctct gatttaacgt
     6421 atgtcgccaa tttcctgatg aaggtgactg gcgacttgct gttgtatgtc cttctcagaa
     6481 aattgttttt gaataacaaa taacaggatt ttataatctc tt
//
LOCUS       DAZKFZ010000239         6478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000239 DAZKFZ010000000
VERSION     DAZKFZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(20..2302)
                     /locus_tag="ACHOQW_004233"
     CDS             complement(20..2302)
                     /locus_tag="ACHOQW_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9575193.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDHIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYGELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKIYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPTEHAAAVLNLIDEGILSWTEPGIMKVLRDLLSNELKCSNRS
                     QQSFTPFNGRSYFPAPSARLTRQERKTIPKIKVLLAQSDIQASYWELEALARGAVLDF
                     GHKRFKFDNDIDFFDRQREW"
     gene            complement(2292..2567)
                     /locus_tag="ACHOQW_004234"
     CDS             complement(2292..2567)
                     /locus_tag="ACHOQW_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP9575194.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2564..2788)
                     /locus_tag="ACHOQW_004235"
     CDS             complement(2564..2788)
                     /locus_tag="ACHOQW_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFP9575195.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKRYRRTA"
     gene            complement(2791..3090)
                     /locus_tag="ACHOQW_004236"
     CDS             complement(2791..3090)
                     /locus_tag="ACHOQW_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFP9575196.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3090..3314)
                     /locus_tag="ACHOQW_004237"
     CDS             complement(3090..3314)
                     /locus_tag="ACHOQW_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFP9575197.1"
                     /translation="MHTVSENKCGKYALLLQQARAEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3378..3878)
                     /locus_tag="ACHOQW_004238"
     CDS             complement(3378..3878)
                     /locus_tag="ACHOQW_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFP9575198.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(3875..4045)
                     /locus_tag="ACHOQW_004239"
     CDS             complement(3875..4045)
                     /locus_tag="ACHOQW_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575199.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4056..4331)
                     /locus_tag="ACHOQW_004240"
     CDS             complement(4056..4331)
                     /locus_tag="ACHOQW_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFP9575200.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4453..4752
                     /locus_tag="ACHOQW_004241"
     CDS             4453..4752
                     /locus_tag="ACHOQW_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575201.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            4868..5881
                     /locus_tag="ACHOQW_004242"
     CDS             4868..5881
                     /locus_tag="ACHOQW_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9575202.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6146..6463)
                     /gene="yegR"
                     /locus_tag="ACHOQW_004243"
     CDS             complement(6146..6463)
                     /gene="yegR"
                     /locus_tag="ACHOQW_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP9575203.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         1749 a         1637 c         1422 g         1670 t
ORIGIN      
        1 gtttgaccgt ttttttcaac taccactcac gctgtctgtc aaaaaagtcg atatcgttat
       61 caaatttaaa acgcttatgc ccaaaatcca aaacagctcc gcgagcgaga gcttcaagct
      121 cccaataact agcctgaatg tcactttgtg caagaagaac cttaattttc gggatagtct
      181 ttctctcctg ccttgtcaac cgggcggatg gggcaggaaa gtagcttcga ccattgaaag
      241 gggtaaagct ctgctgtgaa cgattactgc atttcagttc attactcaat aagtctctaa
      301 gtaccttcat aatgcctggc tcggtccaac ttagaatccc ttcatctatt aaatttaaca
      361 ccgctgcagc atgctcagtc ggtgtgggag ccggtaacga agtatcacca ccggtgagtt
      421 ttccacagtt attgacagga ctccgaggcg cggcgatgcc gctttttaaa gtcaaaggct
      481 caacgaccgg aactttcggc acaatgcgcc agtccgtcgt tctggtgata tgaatatgac
      541 gcgcgccgag atgcggcgcg taaatgccga ccactctctc gacttcttcc tcgtactcgt
      601 taacgtcatc cgacgggcta cgggcgaccc tgacagtctg acaatcgcgc gggacatttg
      661 ccccaccctg cgcgctgata tacaacgcaa aatcaccact gtctgcggca gcgcgagcag
      721 cctcgacgcg ttcgtcaaac tcatcagcaa tactgacgcc gcgaggcaat ttgcgtagtt
      781 cacggtaagc ccccattgtc ggcagtccaa ccgttttaaa ttgcggaatg cgccacgttg
      841 acgcccatgc ggtaacagcc gcggcagtat ctttaagcgg cttaccggta tcgttatcga
      901 gctgaccatc cagtgcatag ccgtcgatgt tttttgaaat gtatttcgcg atatatcccg
      961 cagcaccgcc ccggttaagg tgttttgcct gaaaacggtt tcgcgcagct cctctttcgt
     1021 ctccatcctc tttgagcgcg tagcgacgca tgatttcgat aatctggtta cgctggcgtg
     1081 gattacaaaa aagcatcata tgccagtgcg gcgttccgtc gtggtgtggc tcgacgacac
     1141 gcaaaccgta gacctgtaaa tcattatcct tgaatgccgt gcgcatcagg ctccagatgc
     1201 ggcagagata acgctgcgca tcctttggat taaatgcctc atcgttccag ccgtgattaa
     1261 gctgaacggt tttactttcg ccttttctga cctgacgtgt cgggtgatac tttgacggcg
     1321 cggtcagcgt gataaacatc cccacatcac cctctgcggc ggcgtaacgc tcaataccgg
     1381 caatggtgtt catcagctcc atccggcgaa tttcaggatt agaaatactg cccatcacct
     1441 tactgataag gtcgatgcgc tcgccggttt ccctgttttc aaggtcacac gatttaagaa
     1501 attccagatt tgcctggcgg cgcgcacgca catcacgaat ggcgtgttta ctggcataag
     1561 gagaacggtc tttattgacc tccccgacag ctatcagtaa cgcttcatgc cagcgcatac
     1621 gctggccttt aagctgatga gtccaccact catcgttaaa cagacgggca atggcagaat
     1681 atgcctgcct cgtggtcatc tgccctttac ggtatatttt ccagtagaga ggggaaatat
     1741 tgaaagcacg tgcagcgcca gcaacatgac catagaggtg agcctgcgcc tcatccgtaa
     1801 acagcgattc tttttcgcca tgcgcatcca cccaggcatc actgagttcc ccatacatca
     1861 tgaaaagctg cgatgagata cgggcagcaa actttttcag ctccttgtca ttcattcccg
     1921 gcaggcgcgc atagtggtca cgctctgcca gaaacagtaa cgacgcgtcg gtgttcattt
     1981 catggcgctg attcacacgc tcaatgcgcg gccataaacg acgctgaaaa gtggatgtga
     2041 ggaaataaaa cccgtgcacc gggcttttat tgcgccggat gtggtcatag cgtgaagtaa
     2101 acagcgagcg caaaaagtaa ggcaggcggt taatcgtgga taaaacacct tgcacctgac
     2161 gcatctcgtc acgtgtaagg ggtctttcgc gcccgacggc ctcgcgtggc gcgttccatg
     2221 cataagcacc ggtaaacgcc ttaccggtgc ctgcagcaaa tgctgacgga gggacaaaac
     2281 gcccggaggc tttaacggcc atatgagcca aaagcctctg agcaacgctt gctgagttgc
     2341 tcaacctgcg cgtttaaatc agcaaaagac tttgcgcttc cggtcagaat atcgtgatgc
     2401 atcaggccgg aaacgagctg gcttaatttc gggtaataac caaccaccgc cagccattcc
     2461 tgaccggcgt ttttaccgct ttccgctctc tttttctcgt ggagaataaa ctgaaagctg
     2521 tcactggtaa cgacataacg ttcgccaatt tcaatacgaa tactcatgcc gttctccggt
     2581 aacgtttgtt ttttgcttca aagactgact gacaggaaac acaacgcgtg gctgacgggt
     2641 aagccgcacg acgggcagca ggtattggcg cgtcacactc ttcgcaaacc agcgcagaag
     2701 caccgcaatg ttttaccctt gccgcgttaa tctggcgctc cagtaattca gcctgttgtt
     2761 cctgaataaa atctacgttg tccggcatta tcagctcctt ttatcgttaa gtttcctgga
     2821 tacatcagcg caataactgg caagttctgt cgttaatttt gtcagttcat ccactgagga
     2881 aatttgcttg tggaatacag cgcgtttaac aagtaaattg accacatcag acaggaggtt
     2941 taattcattc tgataaatcg cgataacaga ttcagttatg tcgcgttttt ctttatcaag
     3001 gcaaagttga ataagagaca aatcgccatt tttcataacg gcgattttta aggcgttatt
     3061 cagtaataca actgaatgag aacaggacat caaagcacct ccccgcgaga caatccgata
     3121 ttgtgaaatt tttccgactc ctgactgagc agctcgacta tctccacgcg ggataactcc
     3181 gcctttgtga tatggcgaat catggcgtca agatgagaag aaaagcgtgt cgctgcgtcg
     3241 gcctgtgctt cggctctggc ctgttgcagc agtaatgcgt atttaccgca cttgttttca
     3301 gaaactgtat gcatgacttt ctccaggcaa aaagaagccc cgcacgatta agtgcgttaa
     3361 aaactctggt taattattta atgcagatat tgctctggtt ttaccgacgt cagaattgtc
     3421 ggtgcatact caaacaggct gaataattca cgtaatgcac ggaataaagc atcacgccag
     3481 taacatgatt cttcattaat tcgccagtat ggctggttga attctttttc agtcaatccg
     3541 gcatgcataa ataaagtacg acgctgactg actgttaaaa aactaatata tgcatactca
     3601 cttgcgccaa cctgacggcg ttttgagaat gccccacgca attcatcaat tgcacaaacc
     3661 agccgttcac gttcgacgtc gttcatttct tcaaaacgca tcgttgcgtg acgctgtttt
     3721 aactgcgcat ggaagcaaac cgttagccgt tcgcgttcca tcatctgatt ataataatca
     3781 catgtatcct gccagcgagg gacggcaaga tgcttgccaa ttatccggcg catagctgct
     3841 ggctgttttt caacgagatt gagcgtcatc actgtcattt ccagaccctc cggcttttca
     3901 gaaaggtcag agcctttttt aacggactct gttttttggt gcggataatg attcccttac
     3961 gccccttacc gtgggtgatg gtgaagtcaa tcgccctggg gctttcgtta cgcaataact
     4021 gagcaataca acgaggctca ttcatacggt tctccttaac gtggttcacc gagacctaac
     4081 cacatcaacc agccgtcacg aatctcttta ggacggcttt cataagccag ttttagtccg
     4141 ttattccatg ccggaaggta tacccaatat tcaccagcac gccccgatac tgactgaggg
     4201 tcagtaatct caataactgg taatttccct ttctcaatca tgccccttac agctcttgga
     4261 gttttaccaa tgagttttgc aaactcctga taaggcacgg catcagtcac gcttacaagc
     4321 tgtctattca tctgctacga ttctccctta gtgcttctaa tggctcctaa tggctaatta
     4381 ttgcctaaaa ggataactcc agaagcgcaa catttcacac tatcagcaag aaattacgca
     4441 atcggagtaa ttatgtcaat agacgtttcg gagaagttga agctaatccg tgaatctgaa
     4501 aggttaaacc gtaaagaatt cagtgaatta actggtgtag cctacagctc actttcgagc
     4561 tatgagagcc ggtcaaaaaa cgctggagtt gaagccataa tgaaggtctt acaacatcct
     4621 agatttacta aatatacttt gtggttcatg actgatcagg tagctccaga agccgggcaa
     4681 attgcgcccg ctctcgcaca ctttgggcaa aacgaaacaa cgtcgcccca ctccggtcaa
     4741 aagactggtt aacaatttat cgtgaatata ttcatcacaa gtgcctacta ttggtggcta
     4801 aatttcagcc accacgaaaa aagcgattag tagtagcaaa aaaaagtacc actcggaggg
     4861 ttttctgatg gcaatcaaaa aactcgatga tggtcgatat gaagtggaca tccgccctac
     4921 tggacgtaac ggaaaacgca tccgtaggaa gtttgataag aaaagcgaag ctgtcgcttt
     4981 cgaaaaatac acgttgtaca accaccacaa taaagaatgg ctatcaaaac caacagacaa
     5041 acgacgtctg tcggaactga cacagatctg gtgggattta aagggtaaac acgaagagca
     5101 tgggaaatct aatcttggaa aaattgaaat cttcacaaaa ataacgaatg acccatgcgc
     5161 atttcaaatc acgaaatccc ttatcagcca gtactgcgcc acccgaagaa gtcagggtat
     5221 taaaccttcg agtatcaatc gtgatttaac atgtattagc ggcatgttta cagccctgat
     5281 tgaagcggag ttattctttg gtgagcatcc tatcagaggg acaaagaggc ttaaggagga
     5341 aaaaccagaa acaggctatc tcacacagga agaaatagcc ttactgcttg cagcacttga
     5401 cggcgacaat aaaaagattg cgattctttg cctaagtaca ggagcacgtt ggggagaagc
     5461 agctcgtttg aaagcagaaa atatcatcca taaccgcgtc acgtttgtta aaacgaaaac
     5521 aaacaaacca cgcaccgtcc cgatctcaga ggctgttgcc aaaatgatcg cggataacaa
     5581 acgaggtttt ttattccctg atgctgatta ccctcgcttc agacgaacaa tgaaagcaat
     5641 aaaaccggat ttgccaatgg ggcaagccac acatgcacta aggcacagct ttgccactca
     5701 tttcatgatt aatggaggaa gtattatcac gctacaacgg atactaggtc acacgcggat
     5761 tgagcaaact atggtttacg ctcattttgc gccagagtac cttcaggacg ccatttctct
     5821 taatccgcta agaggtggta ctgaggccga gagtgtccac acagtgtcca cagtagagta
     5881 acgtttaagg gctttcagtg gtaatttatg ccgctcaaac ccgcattgta ccgttgaaag
     5941 cccctactgg tgacacccta aatctccctt acacgggctt attttttatg cataagccct
     6001 atccctgata accgtcttcc attgaccaca tcgatagaat ctcccttcat agcacgatgc
     6061 ctttcacgta acggcatcgt gctcgcacag gttccggcta agcacaacca gaacgcgcat
     6121 gtttgacgct taccaaaaaa tattctcact ctccacattt gaatgtcaga cgagcgacgc
     6181 catgtaatcc tgcaccttct gtcttcaggt caactatctg catttttttg ccctgagtaa
     6241 cacagaaatg ggctgcatca ttttttacta tattttctgc accagatatt ctgcccctgg
     6301 ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag tttacgctga atattacttt
     6361 tatatgcaag accaaattta ccgatacttg tctcatcatt atgcacagca caaccagaca
     6421 taagaaaaac actaattaat gatatagcag ctatcttttt catctcacct tcccccat
//
LOCUS       DAZKFZ010000240         6405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000240 DAZKFZ010000000
VERSION     DAZKFZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..728
                     /gene="gadW"
                     /locus_tag="ACHOQW_004244"
     CDS             <1..728
                     /gene="gadW"
                     /locus_tag="ACHOQW_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP9575204.1"
                     /translation="THVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCS
                     PDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESGV
                     NSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAER
                     MYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFI
                     NTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1091..4204)
                     /gene="mdtF"
                     /locus_tag="ACHOQW_004245"
     CDS             complement(1091..4204)
                     /gene="mdtF"
                     /locus_tag="ACHOQW_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP9575205.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4229..5338)
                     /gene="mdtE"
                     /locus_tag="ACHOQW_004246"
     CDS             complement(4229..5338)
                     /gene="mdtE"
                     /locus_tag="ACHOQW_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP9575206.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5446..5724
                     /locus_tag="ACHOQW_004247"
     CDS             5446..5724
                     /locus_tag="ACHOQW_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575207.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5725..6252)
                     /gene="gadE"
                     /locus_tag="ACHOQW_004248"
     CDS             complement(5725..6252)
                     /gene="gadE"
                     /locus_tag="ACHOQW_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9575208.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1589 a         1573 c         1570 g         1673 t
ORIGIN      
        1 tgactcatgt ctgctcggtg atcctcattc gtcgttcatt cgatatttat catgaacagc
       61 aaaaaatatc gctgcataac gagagtatcc tgctgctgga gaaaaatttg gcagacgatt
      121 ttgcgttttg ttcaccggat acgcgacgac tggatatcga tgagctgaca gtttgccatt
      181 acttacaaaa tattcgtcag ctaccacgca atttagggtt acatagcaaa gaccgtctgt
      241 taattaacca gtcacccccc atgccgctgg tgacggcgat ttttgatagc ttcaatgaat
      301 ccggggtaaa ttcaccgata ctgagcaata tgctctacct ttcctgttta tcgatgtttt
      361 ctcataagaa agaactgatc cccttacttt tcaatagcat cagcactgtt tcaggaaaag
      421 ttgaacgcct tattagcttt gatatcgcca aacgttggta tctgcgcgat atcgcggaaa
      481 gaatgtatac cagcgagagt ctcatcaaaa aaaagttgca ggatgaaaac acctgtttca
      541 gtaaaatatt actcgcctcc aggatgtcga tggccagacg attactcgag ttacgtcaaa
      601 ttcctctgca tactattgcg gaaaaatgtg gctatagcag tacgtcgtac tttataaaca
      661 catttcgaca atattatggt gtaacgccac atcagtttgc gcaacattcg ccaggtacct
      721 tttcctgaca tattttgcat ttgaatattg gtcaggatct cacacctgct tcatgtgaaa
      781 ctcttccctg atgatttctg ccgggctacc ggctagttct ctttcgcagt ttgccaggcg
      841 gtataggtat tttcaggagg aaaagactcg gcatgtttgg gattattaag ctgacaattc
      901 atacgattac gggaatgtgg gtcagtattg tgctgttcaa attgatgacg aatggctgga
      961 gtggtttcta ctttcagtgc tgtgtactaa gcctggtatt cctgacggtt agctggttgt
     1021 tgagtggtga atggttagca ggaaagagta aggctgaacc ttcacgttca accttactct
     1081 catttacacg ttacgctttt ttaaagcggg caaagagatg ttccactaca acgaaaaaga
     1141 ccggaacaaa gtaaattgcc agcactgttg cggcaaacat cccgcccatc acgccggtac
     1201 ctaccgcgtt ttgcgcgcca gaaccggcac catgactgat aaccagcggc agcacgccga
     1261 gaataaaggc cagagaggtc atcaggattg ggcgtaaacg catccgcgcc gcttcgatga
     1321 ttgcctctat cggcgttttc ccttctttct gcatcatctc aacggcaaat tcgacaatca
     1381 ggatggcgtt cttggcggaa agcccgatgg tggtcagcaa accaacctgg aagtagacgt
     1441 cattacttaa gccgcgcaga tcggtggcca gtaatgcgcc aacgacgcct aacggaacaa
     1501 ccaacatcac cgagaacgga attgaccagc tctcataaag tgcggcgagg gcgaggaaca
     1561 ccacgaccag tgaaatcgca tacagcgcag gagcctgatt tgaggataac gcttcctgat
     1621 acgacagtcc ggtccatgag tagccgacgc ctgccggaag tttagcgacc aggtctgcca
     1681 taaatttcat ggcgtcaccg gtacttttcc cggccgccgc ttcacctaaa atctccattg
     1741 acgggatgcc gttgtagcgt tccagtcgcg gtgaaccata ggtccattcg gtagacgagt
     1801 aggcagaaag cggtgccatc gtgccagagg cgttgcgtac ataccattgg ttgatgttat
     1861 ccggcaacat acggaacggc gtgcctgcct ggacatacac ttttttcacc cgcccctggt
     1921 tgaggaagtc gttcacgtag ctgctgccga aggcggtgga aattgtctgg ttgatatcag
     1981 acagcgccac acccattgcc tcagctttcg cggcgttgac gttcactttg aacatcggcg
     2041 tatcttccag gccgttcggg cgtaccccgg tgacctgatt cggtgattgc gctgccagtg
     2101 ataacagctc gtttcgtgcc tgggttagtt tttcgtgccc caggttaccg ttgtccagca
     2161 gttccatatc aaaacctgac gcggtaccca gttcagccac cgcgggtaag ttgaacggga
     2221 agacgacggc tttattgata ctgcttagcg caatcattgc ccgctgaatg atcgcggtaa
     2281 ccgagttttc ctcaccgaca cgttcagacc acggcttgag actgataaac gccagaccgt
     2341 tgttttgccc ctggccgctg aagccaaagc cgccaacggt aaacaccgac tgaacattat
     2401 ctttctcttt tgtaagataa taatccgtca cctgttgcag cactttcgtg gtgttaacca
     2461 tggtggcacc ggaaggtaac tgcgcggtgg tcataaatac cccctgatcc tcttctggta
     2521 agaaagaggt cggcgtgcgc aggaacagca ccgccatccc ggcgcaaatc agcaggtaga
     2581 tcaccatgta gcgaccggta caacgcaaca gtgagcgggt gctatcggta tagtgctgag
     2641 ttgatttttc aaacagcgtg ttgaagcgtg cgaacagggc attaggtttg tgaccgcctt
     2701 ccggcgcggc tttcagaatg gtggcgcaca gggcaggggt caggctcatt gccacaaata
     2761 ctgaaagcag catggaggag atcagcgtga tggagaactg gcggtagatc tccccggttg
     2821 caccgctcat aaaggccatc ggcataaaca ctgcggaaag aacaacggca ataccgacca
     2881 gcgcacgttg gatctgcccc atcgatttat gcgtcgcttc cttcggtggt agcttatctt
     2941 ccgcaatgac acgctcgacg ttctccacca ccacgatggc gtcatccacc agtaacccta
     3001 tcgccagcac catcccgaac atagtcaacg tgttgatggt gaaaccgacc gccgacaaga
     3061 tcgcaaacgt cccgagaata accaccggta cggcaatcgt cgggatgatt gtggcacgga
     3121 aattctgcaa aaacagatac atgaccagga agacgaggat gatagcctca accagtgttt
     3181 tgaaaacttc ctgaatagaa atttcgataa acggcgtggt gtcgtaagga taaaccgtct
     3241 tcagacttgc cgggaaatag gctgataagc ggttcagttc ctctttgact gcccgcgagg
     3301 tatccagggc gtttgctccg gtagccagtt tgatggcgat cccggcagca ggtttgccgt
     3361 tatagcgcgc cacggtggaa taatcttccg ccccaagttc gacgcgggcg acatcacgca
     3421 gcagcacttg cgaaccatct tgctgaactt tcaacaggat tttgccaaat tcttccggcg
     3481 tttgcagacg cgtctgcaca atgatcgagg cgtttagttg ctggtctgcc gcctgtggca
     3541 tgccacccag ttgaccacca gaaatctggt tgttttgcac cttaatctgg gaaataacat
     3601 cggaaggtac cagattgtat ttattgagtt tttgcgggtc cagccagata cgcatggcgt
     3661 actcggaacc aaagagctgt acgctaccaa cgcccgcggt acggcttagc gggtctttga
     3721 tattagacgc tacatagtcc gcgatatcgt actggttgag gctgccgtta tcagaaataa
     3781 acgccgctac catcaggata ttactgctcg atttatcgac gctaatcccc tgctgctgca
     3841 ctgcttcagg taatgaaggc atagcgagtt gcagtttatt ttgcacctga acctgtgcga
     3901 tatcaggaga tgtcccagtc tcgaaggtca gagtgataga ggcattaccc gccgcatcac
     3961 tggttgaaga catgtacatc aggccatcaa gcccattcat attttgctca atcacctgag
     4021 tgaccgagtc ttctaccgtt tgcgcatcgg cacccggata ggtagcgctg acggtaatgg
     4081 tcggtggcgc aatctgcgga tactgcgcaa ccggtaagtt catgatcgcc agaccacctg
     4141 caagcatcat aataatggca agtacccagg caaaaaccgg gcgatcaata aaatagttag
     4201 ccatgaaagt ccccttaagc ctgcaacgtt attgtttcga ttcggtgctg gcgttttcct
     4261 ggctggagga aattgctcgt gctttgatac ccggacgaat gcgttgcaaa ccggaaacga
     4321 tcacccgatc gccagcctgc aagccagagg tgacaaccca ctggtcgccg atggctttgc
     4381 tggcttcaat ttcgcgtagc ttcacgacat cgtctttatc cagaatgagc gccgttgctt
     4441 taccttgggc gttgtgggtg acgccttcct gcggcaccag taatacattc tggcggctac
     4501 cttcatccac taatgccgtg acatacatgc caggcagcaa gtcaccattt gggttgggga
     4561 aaatcgcccg taacgtcacg gagcccgtgg tttcatcaac cgtcgggtcg gagaatttca
     4621 gcgtgccggt ctggctgtaa cgtttaccat tttccagatt gagctgtact ggcgtactgc
     4681 cctgaacctg tttgatttgc ccactggcga cctcttcttt catgcgtaag aaatcttgca
     4741 ccgactgcgt gagatcgaca taaatcgggt ccagacgttg tacggtaacc agcgaatctg
     4801 cctgattagc ggtaacgagt gcgccgacgg tcaccgacga tttcccgctg acgcccgtaa
     4861 tcggcgaggt gacattcgcg tattgcagat tgatcgtcgc ctgttcaaca gccgctttgg
     4921 cgacggtgac attggcttct gcttcattca actgggtgcg cgcggtgtcg taatcctgac
     4981 ggctaacgta gttggtcttc agcaacgatg cctggcggtt aaaggtgatg cgggcattgc
     5041 tggcggtaga gagcgctttc gccagcgagc ctttggcgga gtttagctcg gcctgtaaag
     5101 gtgcaggatc aatctgatac agcgaatcgc cctggttcac tttatcgcct tcgataaagt
     5161 tgcgtttaat gataataccg cccacctggg gacgtatctc ggcaacttca taaggaacgg
     5221 ttctaccggg caattcgctc aacacattga ccgacccagg ggagagtgtg acgacaccga
     5281 cctcaggcgt catggcggcg gtgttttccg ccgatttgtc atcgcaggcg gtgagcatcg
     5341 cgccgcagaa taacaacggt attaacagct ttcttcttct gttcatttta gtccctgaaa
     5401 attcttgaga aataacggtt ctttaaattg cgcgcgagga gagatatgag ggatttacag
     5461 accagcggca tagtggggtt gtcagccagt aaggtgggat acaggcatag tgatcgacat
     5521 ggtgaggtca acgacaaaaa aaatgttgcc gttctgccaa cagttcctgc cagcattcgg
     5581 gcaacgaagg gttctactgg tggatacaca taccagggga ataaagtcgt tttagcctct
     5641 cctttagtaa tcaccggggg taatgaaatc agcatctgca tccctcgtca tgccagccat
     5701 caatttcagt tgcttatgtc ctgactaaaa ataagatgtg atacccaggg tgacgatgtc
     5761 gctcatacgt cttagtttca gcttgtacat aatgttgaat ttctgaatct ttaatgtttg
     5821 ttgcgaaata ttgagcgtcg acgtgatatt gctttcattt ttatttttca gaatcagttg
     5881 cataattttg aactgattat gtgaaaaaca taattgtgaa ttcttatggg gcaagtgttt
     5941 accataagta atctcaagaa gatttttgat actttctttg cggctaacaa aataaatagg
     6001 cgctttagct tttagtttac tgatgtgata aggtgccaaa acaataattc tctcggcatc
     6061 taatttctcc agaaatttaa tcgcttcttc atcaaggata tgattgtgat aggtatcaat
     6121 gataaccttg aagggtttat tgcctacttt tttgatctct gacagggaat tgatttttat
     6181 catttcgtga ttatctttca actgccaaaa gccctgtaaa agaaaagaat ctttcgtcat
     6241 gagaaaaatc ataacttgct ccttagccgt tatcgtttcg aacatgtcat ccttttcatt
     6301 atttacatcc ttgtccgaat cgttgttcaa tatagtaaac gcctataaga actttgccct
     6361 tcgtgaacta atacgtctat taacactaat caatacttca gattt
//
LOCUS       DAZKFZ010000241         6202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000241 DAZKFZ010000000
VERSION     DAZKFZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..197
                     /locus_tag="ACHOQW_004249"
                     /pseudo
     CDS             1..197
                     /locus_tag="ACHOQW_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(201..1592)
                     /gene="tcyP"
                     /locus_tag="ACHOQW_004250"
     CDS             complement(201..1592)
                     /gene="tcyP"
                     /locus_tag="ACHOQW_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP9575209.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1725..2315)
                     /gene="ydjM"
                     /locus_tag="ACHOQW_004251"
     CDS             complement(1725..2315)
                     /gene="ydjM"
                     /locus_tag="ACHOQW_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9575210.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2478..3146)
                     /gene="hxpB"
                     /locus_tag="ACHOQW_004252"
     CDS             complement(2478..3146)
                     /gene="hxpB"
                     /locus_tag="ACHOQW_004252"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP9575211.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3293..3829
                     /gene="yniB"
                     /locus_tag="ACHOQW_004253"
     CDS             3293..3829
                     /gene="yniB"
                     /locus_tag="ACHOQW_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP9575212.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3870..4730)
                     /gene="yniA"
                     /locus_tag="ACHOQW_004254"
     CDS             complement(3870..4730)
                     /gene="yniA"
                     /locus_tag="ACHOQW_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9575213.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4836..5126)
                     /gene="ghoS"
                     /locus_tag="ACHOQW_004255"
     CDS             complement(4836..5126)
                     /gene="ghoS"
                     /locus_tag="ACHOQW_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9575214.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5227..6156)
                     /gene="pfkB"
                     /locus_tag="ACHOQW_004256"
     CDS             complement(5227..6156)
                     /gene="pfkB"
                     /locus_tag="ACHOQW_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP9575215.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1589 a         1563 c         1534 g         1516 t
ORIGIN      
        1 tcacttagaa aacgtctcgg gtatgaggtt ttaagcttct cgctacaggc acattcattg
       61 agccaggaat gtattgataa gttggacatt ttttcgctga cgatctgttt aaatatgagt
      121 cagtatgcat cgccgcgatg gaacatttaa aaagtaaggc aacggcccct ttacaaaacg
      181 gaccgttgcc agcataagaa ttaatggtgt gccagttcgg cgtcgtcttc gctatccaga
      241 atggctttat cggtttgctt cagccactgg ctggtcagcg tgccagctgt catcgagcca
      301 ctaacgttta gcgccgtacg gcccatgtcg ataagcggtt caacggagat taacagcgcc
      361 accagggtta ctggcaggcc catcgcaggc agcacaatca gtgcggcgaa agttgcacca
      421 ccaccgacac cggcaacgcc tgcggagcta acggtaacaa taccgaccag cgtcgcaatc
      481 cacatcgggt ccagcgggtt aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc
      541 ggatacaaac cggcgcagcc gttctgacca atggtcgcac cgaaagaggc ggcgaaactg
      601 gcgatggatt caggaacacc gagtcgacgc gtttgtgctt ccacattcag tgggatagac
      661 gcagcactgg aacggctggt aaaggcaaac gtcagcacag gccatacctt acggaagtac
      721 ttcagcggac tcacgccatt aatgcccagc agaatgccat gcactgcaaa cataatcagc
      781 agaccgaggt aggacgcgac aacgaaactt cccagtttga tgatgtcttg caggttagaa
      841 cctgcaacca ctttggtcat tagtgccaga acgccgtaag gggtcaactg catgaccagg
      901 cgaactagtt tcatcaccca gctttgcagg gtatcgatag cggttaagac gcgttcacct
      961 ttcggcgcat catccttcag cagtttcagc gcagctacac cgaggaatgc ggcaaaaatt
     1021 accacgctga taattgacgt cggattggct ccggtcagat cggcaaacgg gtttttcggg
     1081 ataaaggaca agaccagctg cggaacgctc aggtcagaga ctttaccaac atagttactt
     1141 tcgatggcgt tcagacgtgc agtttctgca ccaccctgaa ccagaccttc agccgtcaaa
     1201 ccaaacaggt tggttaccag cacaccgacc agcgccgcaa tcagcgtggt aaacaaaagc
     1261 gtaccaatgg tcagaaaact gattttgcct aactgagatg cgttatgcag acgggcaacc
     1321 gcgctcagaa tagaggcgaa gactaacggc ataacgatca tttgcagcag ttgaacatag
     1381 ccgttaccaa caatgttaaa ccactgtaca gaatctttaa gtacctggct gtcagaacca
     1441 taaatggtat gcagggcaag gccaaaaacc acacccatca ccagacccac cagcactttt
     1501 ttcgccagac tccactgttt atggcgggtc tgagccagcg caaacagcag tacaacgaac
     1561 accacgatgt tcgcaattaa tggaaagttc atccccgttc tcctgatctt attatttcta
     1621 tcactgcgta gcagtcatag tgttggcgca agattaacag aactgtgagc tggctcttat
     1681 attcaaatgg aattgcttat tacagaaagg cgatttttgc ctttttagta ttcaacctga
     1741 tgcttaataa gccgatgaaa ctggatctgc aaggtattga tcatttgcga tgaccaacga
     1801 acagcgctgt tttcgggtaa tgaatgaggc atccataccg accagacaaa taatgccatg
     1861 cagataaaac gttccagttg gttacccttt tggggaacca ggataggcaa gcggaaacgc
     1921 cagcggcatg gccagagcag gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg
     1981 cttaaataac ccagcaccat tccttgtagc gcatcagccg gaatgaacca gctttcagga
     2041 accttaaggt aaaaggttgc cagcagcgca aataccgcca gcagactgtg ggtaaaacca
     2101 cgatgcccaa aagcgcgggc gatcggtttt gatatccact ttaatcgctg cccaagaaac
     2161 gactttgggt gatcgatgtc cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc
     2221 caccagtcac cctgtgccag cacgggcgtc agctcggcat ttttggcaaa taccgcacaa
     2281 gcaatagaaa agagaaggtg accttccgcc gtcatgataa aactcactaa actgtcgata
     2341 cgtacagtat aggattttta tacagtgcac ggaagtggtg acggtgtaac tctttctctt
     2401 aaattgacga ttaatccgtc agttgaatgc aaattcagat aaagaaccag gggcagcgtg
     2461 atcactgccc ctggtcatta accgagaagg tcttttgcgg tgagttctgt cagcgatgag
     2521 agtttgacgt ttgctaatac aaaacgtgga tcattttgcg cttctggcgc aggaacgacg
     2581 atggaacgca tgcgggctgc tttagaggcg atcatgccat ttaccgaatc ctccagcgct
     2641 acgcaggtca gtgggtcaac gcccagtttt gctgcgcagt cgagatatac ttgcggatgc
     2701 ggcttgctgt aaggcagttt ttcggccgag gcgagggcat cgaaactgtc gcgtaagtca
     2761 aacatggtca acactttttc cagcatatgt agtggtgacg cggaggccag tcccaccaat
     2821 aaaccttgtt ctttgcataa cgcaacggct tcgcgcacgc ctggtaataa tggacgtgtc
     2881 tcttcaacca gtgaaatggc acgggcaata acccgttcta ctacttcctg acggcttggc
     2941 ccattccacg gttgccgggc gtaccaaaga tcgaccacca tatcgatgcg taaacctaag
     3001 gtgtccggca gctcgttacg acgggagata tccaccccca ggcttgccat cacatccagt
     3061 tcggctcgat cccataaagg ttctgagtcg ataagtaatc catccatatc aaaaattgca
     3121 gcaagaatct gacgcggggt tgacattaca acctctcctt tagagcatta tgtgtaaggt
     3181 attaagccgt gcttatgatt aagcgcctat catatcgcct ttaaagaatg ggcgaaaacg
     3241 gatatctaca ggtaaactaa gtgaaacgac gcgtcttaat caaggagaac tcatgacgta
     3301 tcaacaagct ggacgcattg ctgttttgaa aaggattatg ggttgggtga tttttatacc
     3361 tgcattgatc tctacattga tttctttgct gaaatttatg aatacccggc aggaaaacca
     3421 ggaaggcatt aatgcggtca tgctcgactt tactcatgtc atgatcgata tgatgcaggc
     3481 gaatacgcct tttttgaatc tgttctggta taactccccg acgcccaatt ttaatggtgg
     3541 cgtgaacgtt atgttctggg tgattttcat cctgattttt gttggactgg cgttgcagga
     3601 ttccggtacc cggatgagtc gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact
     3661 cattctggaa aaagccaaag gggaagaggg gctaacgcgt gaacaaattg agtcacgtat
     3721 tgttgttccg caccatacta ttttcctgca gtttttctca ctgtatatcc tgccggttat
     3781 ttgtattgct gcaggttatg tgttcttttc tctgcttggg tttatttaat caaaaggccg
     3841 cacggagcgg ccattatctt caacccatat cacgcagcta ataatctatc cagtgactgc
     3901 tgagcaataa ccaaatgctg accgccaaat aatcttgcac gatttaacag cgtgtagagt
     3961 tggtaaaccg gttgacgttc aaggaaatcg gcaggtagcg gggatactga ctgatagccg
     4021 tcatagattt gtggcggctg ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg
     4081 tcaccccagt agcaggccgg gtcgaaaatg tacgggccat ccggacccag cgcacagttg
     4141 ccggaccata aatcgccgtg caacagagaa ggctgcggtt gatgtgaggc gagacgctgc
     4201 tgaatatgct cgacgatggc atcgatattg ccgaaagcga tccctttttc tgctgccagt
     4261 tccaactgcc agccaatccg ttgttcagca aaaaacgttg accagcgacg ttgccaggtg
     4321 ttgggctgtg gagttgtgga gagcgagtta tcaaaatcga gtccaaattg tggttggtcg
     4381 ctccattgat gtaaacgcgc aatttgctga ccaagaataa atgcgctatg cgcatccagc
     4441 ggacgaggtg ggagataatc catcaccaga aaactgtagt cacggtcagc gccaactgcc
     4501 caaaccttag gcacggtgac ggttttacta cgcgacagta actccagttg gtcggcttcg
     4561 gcggtaaaac cgggaagcag ttccctttca tcacatttga cgaaaaagtc atgtcctgca
     4621 tagcgcaaat gccatgcggc atggacttct ccgccaggca gttcattacg cagttcgatt
     4681 tcgccttcac ctaactgctc gctcaaaaga cgactgattg cctgccacat gatgtctctc
     4741 ccatgttgtc tgccagatca taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa
     4801 cgcacaccac agactgatta ttgcccaaaa accatttatt ttctgttgct attccattcc
     4861 tcccaggtcg tgatggtgat ttcaggatct ttgccggttg cactttgggt aagccccgaa
     4921 accagttctc taatttcttc ttcactttgg gtactgataa gtccaaaagt gttagtcccc
     4981 agctcatgga tatttccctc atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg
     5041 taattattca actcgttaat ttcagtcaat gtatcctcat gcaacatgac ggttatgaca
     5101 taacggacaa gatcgccact ggccatagtt cacccctttg ttatcacatg acttttgagc
     5161 atagtcggag aaacgcgttg ccgacaggtt ggtgatgatt cccccaatgc tgggggaatg
     5221 tttttgttag cgggaaaggt aagcgtaaat tttttgcgta tcgtcatggg agcacagacg
     5281 tgttccctga ttgagtgtgg ctgcactccc cgcagctacg ccaaaacgaa ccatctcttc
     5341 aagagaggca ttttctgcca gtttcagtgt catcgcgccg accatgctat caccagcgcc
     5401 aacggtactc tggcttttca ccggtggtgg caccacctga atgcagtttt cactatcaac
     5461 acccagcgct ccttgtggac ccagggaaac gacaacccgt ttggccttgc cgctattaac
     5521 gatttcctgc gcggctttgc ggacatcgtc tggctgggtg agttcgcgat tcaccagcgc
     5581 actgagttct ttttggttag gcttaaccaa ctcgatgtta ccaatcgcca gtgctgcact
     5641 taacgcttcg ccagaactgt cgatgatgca gcggatccct tggttttgcg cagcggaaat
     5701 cagttgggtt aatttttcca gcttcacacc tggcggcagg cttccgctta tgaccaggat
     5761 ggccccggat tcaatttcca gaacttgctc ttcaagctgg cgaaactcat cttcatttaa
     5821 tgccgcgcct ggcataacaa aacgatactg ctcaccgctt gcttccacat gtacgtgtaa
     5881 attctgccgg gtccagtctt tggcttctac agtagcgacg gggacatttt catccgccaa
     5941 cagtgaaacc aggtgttcgc cggtcgcgcc acccgccggg aagatcgctg tggcactgcc
     6001 tccaagatgg gcaatggcgc gggcgacgtt gatgccgccg cccccgggtt cgaacaccgg
     6061 tgcggtacag cgcagttttc cttcgggata aatttgcggg gtaattgttg cgctatcgag
     6121 agagggcgca agtgtcaacg tatagatacg taccatcatt tcctcctata ggctgatttc
     6181 agtctggcac cgaatcagcg ga
//
LOCUS       DAZKFZ010000242         6149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000242 DAZKFZ010000000
VERSION     DAZKFZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            247..1119
                     /locus_tag="ACHOQW_004257"
     CDS             247..1119
                     /locus_tag="ACHOQW_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9575216.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLNGHGHSMVITDLPGVGESRDRDA
                     EYETLYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCIASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1492..4352
                     /gene="cah"
                     /locus_tag="ACHOQW_004258"
                     /pseudo
     CDS             1492..4352
                     /gene="cah"
                     /locus_tag="ACHOQW_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
     gene            4463..>6149
                     /locus_tag="ACHOQW_004259"
     CDS             4463..>6149
                     /locus_tag="ACHOQW_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFP9575217.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1519 a         1569 c         1771 g         1290 t
ORIGIN      
        1 ttagatactt taggatttta tttggacaac cccgccccag aagacaattc taccttagtg
       61 tatataagga ccgcttatga catagagcgg actgtcagat gaggtttagt tctgtactaa
      121 tgagccgata caaattagct gcatatacct gcattccaaa taatttcaga cagatattat
      181 cgttgtgaat cccccgccac ctttccggtg cgcggggttt tgtctttttt caccgggaat
      241 acatgtatga atccgtctga tgccattgag gcaattgaaa aaccgctctc ctccctgcct
      301 tactcacttt cccgtcacat cctggaacat ctgcgcaaac tcaccagtca cgaacccgtg
      361 cctggcatta tgggtaaaag cggggccggt aaatcctcac tctgtaatgc gctgtttcag
      421 ggggaggtca ccccggtcag tgatgttcac gccggcaccc gggaagtgca gcgcttccgt
      481 ctgaatggcc atggtcacag catggttatc actgacctgc ccggggtggg cgagagccgg
      541 gacagggatg cagagtatga gaccctgtac cgtgacattc tgcctgaact ggacctggtg
      601 ctgtggctga ttaaagccga tgaccgggcc ctgtctgtgg atgagtattt ctggcgacac
      661 atcctgcacc ggggacatca gcaggtgctg tttgtggtga cgcaggccga caaaacggag
      721 ccctgccatg aatgggatat ggccggcatt cagccttctc ccgcacaggc acagaacatt
      781 cgcgaaaaaa cagatgcggt attccgtctg ttccggcccg tacatccggt tgttgccgta
      841 tcggcccgca ccggctggga gctggatacg ctggtcagtg cgctcatgac agcgcttccc
      901 gaccatgccg ccagtcccct gatgacccgc ctgcaggacg agctgtgcac ggaatctgtc
      961 cgggggcagg cccgtgaaca gtttaccggt gcggtggacc ggatatttga cacggtggag
     1021 agcgtctgta ttgcctctgt tgcacgcacg gttctgcgtg ccgtccgtga cacggtggtc
     1081 tctgttgccc gcgctgtatg gaactggatt ttcttctgaa cagtctacca ggggaaatgc
     1141 ctgattacat tgcgttaacc aactgaaaac atgatacaaa cacctcatgc ccctcctgtt
     1201 tctcttgcgt gactgctctg ctgttaatag aataaaacga tcgataaaac aggtatcgat
     1261 cgttactatc gatcgtttat agtggttaat ggcctgcgaa aaggcagctg aatctcttca
     1321 tcatgcagaa cggaattgca cacaacggac tgatacttct ctggcggatg agagagggga
     1381 agcatttatg tccgggaaaa accactttat ctgaccatgc tgttttgtac ctgccggtat
     1441 ccacatttgt gggtaccggc ttttttattc accctctgta aggaaaagct gatgaaacga
     1501 catctgaaca ccagctacag gctggtatgg aatcacatta cgggcaccct ggtggtggcc
     1561 tccgaactgg cccgctcacg gggaaaacgc accggtgtgg cggttgcact gtctcttgcg
     1621 gctgtcacgt cagttccggc actggctgct gacacggttg tacaggcagg agaaaccgtg
     1681 aacggcggaa cactggcaaa tcacgacaac cagattgtct tcggttcgac caacggaatg
     1741 accatcagta ccgggctgga actggggccg gacagtgaag aaaacaccgg tgggcaatgg
     1801 atacagaatg gcgggatagc cggaaatacc actgtcacca caaatggtcg tcaggtcgtg
     1861 ctggaggggg gaacagccag tgatacggtt attcgtgacg gcgggggaca gagcctgaac
     1921 gggctggcgg tgaacaccac gctgaataac agaggcgagc agtgggttca tgagggcggg
     1981 gttgccaccg gtacaattat caaccgcgac ggttaccaga gcgttaaaag tggcgggctg
     2041 gcaacaggaa ccatcatcaa caccggcgca gaaggcggcc cggattctga aaatgtatcc
     2101 agcggtcaga tggtcggggg aacagcagaa tccaccacca tcaacaaaaa tgggcggcag
     2161 gttatctggt cttcaggggt ggcccgggat accctcattt acgcaggtgg tgaccagacg
     2221 gtacacgggc atgcgctgaa taccacactg aatggcggtt accagtatgt gcacaaggac
     2281 ggacttgcgc tgaacacggt aattaacgag gggggctggc aggttgttaa ggcaggtggt
     2341 gctgtcggta acaccaccat aaatcagaac ggtgaactga gggtacatgc cggcggggaa
     2401 gccactgcag tcacccagaa cacgggcggt gcactggtta ccagtactgc tgcaactgtc
     2461 accggcacaa accgcctggg gcatttctcc gtgggaaatg gcatggcaga taacgtcgtt
     2521 ctggaaaatg gcggtcgcct ggatgtactg gaaggccatt cagcgcagaa taccctggtg
     2581 gatgacggcg gtaccctggc agtgtctgcc ggtggtaagg caacagatgt caccatgaca
     2641 tccggtggtg ccctgatagc agacagtggt gccactgttg aggggaccaa tgccagcggt
     2701 aagttcagta ttgatggcat atccggtcag gccagcggcc tgctactgga aaatggcggc
     2761 agctttacgg ttaatgccgg gggacaggca ggcaacacca ctgtcggaca tcgtggaaca
     2821 ctgacgctgg ctgccggggg aagtctgagt ggcagaacac agctcagtaa aggcgccagt
     2881 atggtactga atggtgatgt ggtcagtacc ggcgatattg ttaacgcagg agagattcac
     2941 tttgataatc agacgacaca ggatgccgtg ctgagccgtg ctgttgcaaa aggcgactcc
     3001 ccggtaacgt tccataaact gaccaccacc aacctcactg gtcagggcgg caccatcaat
     3061 atgcgtgttc gccttgatgg cagtaatacc tcagaccagc tggtgattaa tggtggtcag
     3121 gcaaccggca aaacctggct tgcgttcaca aatgtcggaa acagtaacct cggggtggca
     3181 accaccggac agggtatccg ggttgtggat gcacagaatg gtgccaccac agaagaaggt
     3241 gcgtttgccc tgagccgccc gcttcaggcc ggcgccttta actacaccct gaaccgtgac
     3301 agcgatgaag actggtacct gcgcagtgaa aatgcttatc gtgctgaagt ccccctgtat
     3361 gcctccatgc tgacacaggc aatggactat gaccggattc tggctggctc ccgcagccat
     3421 cagaccggtg taaacggtga aaataacagc gtccgtctca gcattcaggg cggtcatctc
     3481 gggcacgata acaacggcgg tattgcccgt ggggccacgc cggaaagcag cggcagctat
     3541 ggcttcgtcc gtctggaggg tgacctgctc agaacagagg ttgccggtat gtctctgacg
     3601 acaggggtgt atggtgctgc aggccattct tccgttgatg ttaaggatga tgacggctcc
     3661 cgcgccggca cggtccggga tgatgccggc agtctgggcg gatacctgaa tctggtacac
     3721 acatcctccg gcctgtgggc tgacattgtg gcacagggaa cccgccacag catgaaagca
     3781 tcatcggaca ataacgactt ccgcgcccgg ggctggggct ggctgggctc actggaaacc
     3841 ggtctgccct tcagtatcac tgacaatctg atgctggagc cacaactgca gtacacctgg
     3901 cagggactct ccctggatga cggccaggat aacgccggtt atgtgaagtt cgggcatggc
     3961 agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc acaacgatat gacctttggt
     4021 gaaggcacct catcccgtga caccctgcgc gacagtgcaa aacacagtgt gcgtgaactg
     4081 ccggtgaact gccggtgaac tggtgggtac agccttctgc tatccgcacc ttcagctccc
     4141 ggggtgacat gagcatgggg acagccgcag ccggcagtaa catgacgttc tcaccgtccc
     4201 ggaatggcac gtcactggac ctgcaggccg gactggaagc ccgtatccgg gaaaatatca
     4261 ccctgggcgt tcaggccggt tatgcccaca gcgtcagcgg cagcagcgct gaaggctata
     4321 acggtcaggc tacgctgaat atgactttct gattatacgg tatcccggaa atacgatttc
     4381 aggaaaattc atgcagtaat gggaaaggac gctcattgct gttcaccgca gttctgaatt
     4441 attgaattta taaaggtgaa tgatgcacga aaagaacatc gccctgcttt gtgatgaagc
     4501 cgaccggctt ttgcaactga atatcaatct gctccggcaa atggttgatg agccggatgt
     4561 gttatctgac agtaagaacg aaaacgggct gctttttgat aaacggaaag cgctgaaaag
     4621 aattgaggag ctggagggcg aacaaatcaa aaccgcccgc agggagatgg tgctggctgt
     4681 ggtcggcacg atgaaggcgg gcaaatcaac caccatcaac gccattgtgg ggcaggaaat
     4741 tctgcccaac cgtaaccgcc ccatgacctc ggtaccgaca ttgatccgcc acgttcccgg
     4801 aaaaacggaa ccggtcctcc atctggaaca tattcagccc gtccgcaatt tattaatcac
     4861 actgcaggaa aaactcgcca ccccggcagg gcagcaagtc gcacagtccc tgcagcaaac
     4921 cggggatacc cgcgaactac tggatattct ggcggatgat gtctggctta aaaatgaata
     4981 ccacggggaa gacgaaatct ttaccggact ggcatcgtta aatgacctgg ttcgtcttgc
     5041 tgcggcaatg gggactgaat ttccttttga cgaatacgca gaagtgcaga aactaccggt
     5101 gatcgacgtg gcgttcagcc acctggtggg aatggatgca tgtcagggaa cactcacact
     5161 gctggatacc cccggcccta atgaagccgg acaaccacag atggaaatga tgatgcggga
     5221 tcaactacag aaagcctctg cggtgctggc ggtgatggat tacacccaga tgaactcgaa
     5281 agccgatgag gaggttcgta aagagcttaa tgccattgct gacgtctcag ccggtcgtct
     5341 gtttgtgctg gtcaataaat tcgatgaaaa agatcgcaat ggtgatggtg cggatgccgt
     5401 acgccagaag gtgccagcaa tgctgaacag cgatgtgctg cccgcctccc gcgtttatcc
     5461 cggctcatca cgtcaggcgt acctggctaa ccgtgcgctt catgagttac gaaaaaacgg
     5521 tgcgcttccg gttgatgaag cctgggtcga tgattttatc agggaagctt tcggacctat
     5581 ggtggaagag gacgactgga aagacagcac taaggtaaat aaaaaggcag aaaaactctg
     5641 gaatatatct ctgatcgatc aactgataac ggaagtcata cagagctccc attccagagc
     5701 ggcggcactg gcggttgact ctgccgccgc aaaactgatg cagaatgcag aaaatgtcag
     5761 cgaatatctg tcattacgtc atcaggggct gcagcaaagc attcagtcac tacaggcgca
     5821 tattaccagc ctgctggcgg atatccagga gattgaggaa tgtcagaatc aggtgaccgg
     5881 tgatgtcaga atggccatgg aggacatcaa taccaaaacc ggggaattac tgacgaaggt
     5941 ctgtgcctca ctggaggagg agctgaacga ctatttcaga agcggtaaac gcaaagaaca
     6001 gcaaatgctg gaggaagaaa attcagcgca gccccgggag agaaacgcat tcgctttctt
     6061 tcacgatatt ttcggaaccg gaaatcagca tgacaggatg cgggactttg atccggacag
     6121 ccccgaaata aaattcagtg atcgcaggg
//
LOCUS       DAZKFZ010000243         6097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000243 DAZKFZ010000000
VERSION     DAZKFZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            246..1349
                     /gene="ompC"
                     /locus_tag="ACHOQW_004260"
     CDS             246..1349
                     /gene="ompC"
                     /locus_tag="ACHOQW_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9575218.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1461..2516
                     /gene="apbE"
                     /locus_tag="ACHOQW_004261"
     CDS             1461..2516
                     /gene="apbE"
                     /locus_tag="ACHOQW_004261"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP9575219.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2590..3654
                     /gene="ada"
                     /locus_tag="ACHOQW_004262"
     CDS             2590..3654
                     /gene="ada"
                     /locus_tag="ACHOQW_004262"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP9575220.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3654..4304
                     /gene="alkB"
                     /locus_tag="ACHOQW_004263"
     CDS             3654..4304
                     /gene="alkB"
                     /locus_tag="ACHOQW_004263"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP9575221.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4380..6023
                     /gene="yojI"
                     /locus_tag="ACHOQW_004264"
     CDS             4380..6023
                     /gene="yojI"
                     /locus_tag="ACHOQW_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP9575222.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1451 a         1537 c         1642 g         1467 t
ORIGIN      
        1 ttattattgc ttgatgttag gtgcttattt cgccattccg caataatctt aaaaagttcc
       61 ctcgaattta cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta
      121 tcatattcgt gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat
      181 gagggttaat cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta
      241 ataacatgaa agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag
      301 caaacgctgc tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag
      361 acggcctgca ctatttctct gacgacaagt ctgtagatgg cgaccagacc tacatgcgtc
      421 ttggcttcaa aggtgaaact caggttactg accagctgac cggttacggc cagtgggaat
      481 atcagatcca gggcaacagc gctgaaaacg aaaacaactc ctggacccgt gtggcattcg
      541 caggtctgaa attccaggat gtaggttctt tcgactacgg tcgtaactac ggcgtagttt
      601 acgacgtaac ttcctggacc gacgttctgc cagaattcgg tggtgacacc tacggttctg
      661 acaacttcat gcagcagcgt ggtaacggtt tcgcgaccta ccgtaacact gacttcttcg
      721 gtctggttga cggcctgaac tttgctgttc agtaccaggg caaaaacggt agcgtaagcg
      781 gcgaaggcat gactaacaac ggtcgtgaag cactgcgtca gaacggcgac ggcgttggcg
      841 gatctatcac ttatgattac gaaggcttcg gtatcggtgc tgcagtttcc agctccaaac
      901 gtactgatga tcagaacagc ccgctgtaca tcggtaacgg cgaccgtgct gaaacctaca
      961 ctggtggtct gaaatacgac gctaacaaca tctatctggc tgctcagtac acccagacct
     1021 acaacgcaac tcgcgtaggt tccctgggtt gggcgaacaa agcacagaac ttcgaagctg
     1081 ttgctcagta ccagttcgac ttcggtctgc gtccgtccct ggcttacctg cagtctaaag
     1141 gtaaaaacct gggtgtcatc aatggtcgta actacgacga cgaagatatc ctgaaatatg
     1201 ttgatgttgg cgcgacctac tacttcaaca aaaacatgtc cacctatgtt gactacaaaa
     1261 tcaacctgct ggacgacaac cagttcactc gtgacgctgg catcaacact gataacatcg
     1321 tagctctggg tctggtttac cagttctaat ctcgattgat atcgaacaaa gggcctgcgg
     1381 gccctttttt cattgttttc agcgtacaaa ctcagttttt tggtgtactc ttgcgaccgt
     1441 tcgcatgagg ataatcacgt atggaaataa gctttacccg cgtggcactg ctggctgccg
     1501 cgctcttctt tgttggttgc gatcaaaaac cacaacccgc caaaacccac gctactgaag
     1561 ttaccgttct tgaaggcaaa actatgggta ccttctggcg tgccagcatc ccgggcattg
     1621 acgccaaacg cagcgccgaa cttaaagaaa agattcagac ccagctggac gccgacgatc
     1681 agctgctttc gacctataaa aaagattccg cgctgatgcg ctttaacgac tcgcaaagtt
     1741 tatcgccgtg gccggtaagt gaagcgatgg ccgatatcgt caccacctcg ctacgcattg
     1801 gcgcgaagac cgatggcgcg atggatataa ccgtcgggcc gctggtgaat ctgtggggtt
     1861 tcggtccgga acaacagccg gttcaaattc cgagccagga acagatcgac gcgatgaaag
     1921 ccaaaactgg tttacagcac cttacggtca ttaatcagtc gcatcagcaa tatctgcaaa
     1981 aagacctgcc ggatttatat gtcgatctct ccaccgtcgg cgaaggttat gcggcggatc
     2041 acctggcacg cttgatggag caggaaggga tttcccgcta tctggtgtcg gtgggcggcg
     2101 cgctgaacag ccgtggtatg aacggtgaag gcctgccgtg gcgggtagcg attcaaaaac
     2161 caaccgataa agaaaacgcg gttcaggccg tggtggatat caacggtcat gggattagca
     2221 cctctggcag ctaccgtaac tattacgaac tggacggcaa acgtctttcc catgttatcg
     2281 atccgcaaac cgggcgtccc atcgaacaca atctggtatc cgtgacggtg attgcaccga
     2341 cggcgctgga agccgacgcc tgggatacag gtttgatggt actcgggccg gagaaagcca
     2401 aagaagttgt tcgccgggaa gggctggcgg tttatatgat caccaaagaa ggcgatagct
     2461 ttaaaacctg gatgtcacca cagtttaaaa gcttccttgt cagcgaaaaa aattaaagcg
     2521 caagattgtt ggtttttgcg tgatggtggc cgggcagact aaaggctatc cttaaccagg
     2581 gagctgatta tgaaaaacgc cacatgctta actgacgatc aacgctggca atctgtctta
     2641 gcccgcgacc cgaatgccga cggcgaattc gttttcgccg tgcgcaccac aggcatcttt
     2701 tgccgtccgt cttgccgcgc cagacatgct ttgcgggaaa acgtctcctt ctacgcaaat
     2761 gccagcgagg cactcgccgc cggatttcgc ccctgcaaac gctgccaacc tgataaagcc
     2821 aatgcccagc aacatcggtt ggataaaatc acccacgcgt gtcgactgct ggaacaggaa
     2881 acgcctgtaa cgttggaagc cttagccgac caggtggcga tgagtccatt tcatctacat
     2941 cggttgttta aagcgactac cggaatgacg cctaaagcct ggcaacaggc ttggcgcgct
     3001 cgccgtttgc gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat tcttaacgcc
     3061 ggattccccg acagcagcag ttactaccac aaagccgatg aaacgctggg catgacggct
     3121 aaacaattcc gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgag
     3181 ctgggtcgtt gcctggtggc agaaagcgag cgggggattt gcgcgatatt gctgggcgat
     3241 gatgacgcga cattaatcag cgagttgcag cagatgtttc ccgctgccga caacgcgcct
     3301 gccgatctga cgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat
     3361 actccgctga cgttaccgct ggatattcgc ggcactgctt ttcagcaaca agtctggcag
     3421 gcactgcgca cgataccttg cggtgaaacc gtcagttatc agcaactggc taacgccatc
     3481 ggcaaaccga aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa gctggctatc
     3541 gtaattccct gtcatcgagt ggtccgtggt gatggcacac tttccggtta ccgctggggc
     3601 gtgtcgcgta aagcgcaact gctgcgccgc gaagctgaaa atgaggagag gtaatgttgg
     3661 atctgtttgc cgatgctgaa ccgtggcaag agccactggc ggctggtgcg gtaattttac
     3721 ggcgttttgc ttttaacgct gcagagcaac taatccgcga tattaatgac gttgccagcc
     3781 agtcgccgtt tcgccagatg gtcacccccg ggggatatac catgtcggtg gcgatgacca
     3841 actgtgggca tctgggctgg acgacccatc ggcaaggtta tctctattcg cccattgatc
     3901 cgcaaacaaa taaaccgtgg cccgccatgc cgcagagttt tcataattta tgtcaacgtg
     3961 cggctacggc ggcgggctat ccagatttcc agccagatgc ttgtcttatc aaccgctacg
     4021 ctcctggcgc gaaactgtcg ctgcatcagg ataaagacga accggatctg cgcgcgccaa
     4081 ttgtttctgt ttctctgggc ttacccgcga tttttcaatt tggtggcctg aaacgtaatg
     4141 atccgctcaa acgtttgttg ttggaacatg gcgatgtggt ggtatggggc ggtgaatcgc
     4201 ggctgtttta tcacggtatt caaccgttga aagcggggtt tcatccactc accaccgact
     4261 gccgctacaa cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa gaattattat
     4321 tgctgtgcgc gcgaagatgt ttaaactgcg ggctgtaatt cattgtccgg gttttctgca
     4381 tggaacttct tgtacttgtc tggcggcagt atcgctggcc atttatcagt gtgatggcgc
     4441 taagcctcgc cagtgcggct ttaggcattg gcttaattgc ttttatcaat cagcgcctta
     4501 tcgaaacggc ggataccagt ctgctggtgt tgccggagtt tctgggatta ttgctgctgt
     4561 tgatggcagt cactctcgga tcgcaactgg cgctcaccac tttggggcat cacttcgttt
     4621 accgactgcg tagtgaattt atcaagcgga ttctggatac tcacgtcgag cgcattgaac
     4681 aactcggtag cgcctcgttg ctggcgggat taaccagcga tgtgcgcaat atcaccattg
     4741 cttttgtgcg tctgccggaa ctggtgcagg ggatcattct cactatcggt tcagcggcgt
     4801 atctgtggat gctgtcgggc aaaatgttgc tggtaacggc tatctggatg gcagtcacca
     4861 tctggggcgg ttttgtgctg gtggcgcggg tgtacaaaca tatggcgacc ctgcgtgaaa
     4921 ccgaagacaa gctgtacacg gattttcaaa ctgtacttga agggcgcaaa gagctgactc
     4981 tgaaccggga acgcgccgag tatgtgttta acaacctcta cattcctgat gcgcaagagt
     5041 atcgccatca tattattcgc gcagacacct tccatcttag tgccgtgaac tggtcaaaca
     5101 tcatgatgct gggcgctatc ggcctggtgt tctggatggc gaacagtctc ggttgggctg
     5161 ataccaacgt tgccgcgacc tattcgttga cgcttttatt cctgcgtacg ccgctgcttt
     5221 cggcggttgg cgcattgccg acgctgctga cggcgcaggt ggcgtttaac aagctgaaca
     5281 aattcgcgct cgcgcctttc aaagcagagt ttccgcgccc gcaggcgttt cccagctggc
     5341 aaacgctgga gctgcgtaac gtgacgtttg cttatcagga taacgcgttt tccgttggtc
     5401 cgattaatct caccatcaaa cgtggcgagc tgctgtttct gattggcggc aacggtagcg
     5461 gaaaatcgac gctggcgatg ttgttgacgg gcttgtatca gccacaaagc ggcgaaatct
     5521 tgctggatgg caaacctgtc agcggcgaac aaccggaaga ttatcgcaaa ctgttttcgg
     5581 cagtgtttac cgatgtctgg ctgtttgatc aactgctggg gccggagggt aaacccgcta
     5641 acccgcaact ggttgagaag tggctggcgc agctgaaaat ggctcataag cttgagttaa
     5701 gaaacgggcg tattgttaac ctgaagttat caaaagggca gaaaaaacgc gtggcgctgt
     5761 tgctggcgct ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg gcggatcagg
     5821 atccacactt ccgtcgtgag ttttatcagg tgttgctgcc gctgatgcag gagatgggta
     5881 aaactatttt cgccatcagt catgatgatc attactttat ccacgccgac cgcctgctgg
     5941 aaatgcgcaa cgggcaactt agcgagctga cgggcgaaga gcgcgatgcc gcttcgcgtg
     6001 atgccgttgc ccggacggca taatattacg acgctgacct ggcggactgc tgccgccagg
     6061 tcaatatgct aaaaatccct tcatttctaa taaccct
//
LOCUS       DAZKFZ010000244         6071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000244 DAZKFZ010000000
VERSION     DAZKFZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..253
                     /locus_tag="ACHOQW_004265"
     CDS             <1..253
                     /locus_tag="ACHOQW_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9575223.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTIGEENFWIDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            265..842
                     /locus_tag="ACHOQW_004266"
                     /pseudo
     CDS             265..842
                     /locus_tag="ACHOQW_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            839..1234
                     /locus_tag="ACHOQW_004267"
     CDS             839..1234
                     /locus_tag="ACHOQW_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9575224.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVLDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1242..1982
                     /locus_tag="ACHOQW_004268"
     CDS             1242..1982
                     /locus_tag="ACHOQW_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575225.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1998..2420
                     /locus_tag="ACHOQW_004269"
     CDS             1998..2420
                     /locus_tag="ACHOQW_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9575226.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2402..2836
                     /locus_tag="ACHOQW_004270"
     CDS             2402..2836
                     /locus_tag="ACHOQW_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9575227.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2829..5378
                     /locus_tag="ACHOQW_004271"
                     /pseudo
     CDS             2829..5378
                     /locus_tag="ACHOQW_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5375..5704
                     /locus_tag="ACHOQW_004272"
     CDS             5375..5704
                     /locus_tag="ACHOQW_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575228.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5704..>6071
                     /locus_tag="ACHOQW_004273"
     CDS             5704..>6071
                     /locus_tag="ACHOQW_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9575229.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1409 a         1486 c         1988 g         1188 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgt gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggagacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc
      181 gacggatgat ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa
      241 ccgtcgtcgc tgaagggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac
      301 ctcagtcgta tcagcaaaac ggcggtgcct ggtgcgtcag caatggccat taaccgcgtt
      361 gcttcatccg cgatatcgca gtcggcgtca caggttgccc gtgagacaag ggtacgccgg
      421 aaactggtaa aggaaagggc caggctgaaa agggccacgg ttaaaaatcc gcaggcagaa
      481 tcaaggttaa ccggggggat ttgcccgtaa tcaagctggg taacgcgcgg gttgtcctgt
      541 cccgacgcag gcgtcgtaaa aaggggcagc gttcatccct gaaaggtggc ggcagcgtgc
      601 ttgtggtggg aaaccgtcgt attcccggcg cgtttattca gcaactgaaa aatggccggt
      661 ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg gtgaaaatcc
      721 cgatggcggt gccgctgacc acggcgttta aacagaatat tgagcggata cggcgtgaac
      781 gtcttccgaa agagctgggc tatgcgctgc agcatcaact gagaatggta ataaagcgat
      841 gaaacatact gaactccgtg cagccgtact ggatgcactg gagaagcatg acaccggggc
      901 gacgtttttt gatggtcgcc ccgctgtttt ggatgaggcg gattttccgg cagttgccgt
      961 ttatctcacc ggcgctgaat acacgggcga agagctggac agcgatacct ggcaggcgga
     1021 gctgcatatc gaagttttcc tgcctgctca ggtgccggat tcagagctgg atgcgtggat
     1081 ggagtcccgg atttatccgg tgatgagcga tatcccggca ctatcagatt tgatcaccag
     1141 tatggtggcc agtggctatg actaccggcg cgacgatgat gcgggcctgt ggagttcagc
     1201 cgatctgact tatgtcatta cctatgaaat gtgaggacga tatgccaaca ccaaatcctc
     1261 tggcaccggt gaaaggggcc gggaccacac tgtgggttta taacgggaac ggcgacccat
     1321 atgcaaaccc gctttcagac aatgactggt cgcgtctggc aaaggttaaa gacctgacgc
     1381 ccggcgaact gaccgctgag tcctatgacg acagttatct cgatgatgaa gatgcggact
     1441 gggccgcgac cggacagggg cagaaatccg ctggagatac cagcttcacg ctggcgtgga
     1501 tgcccggaga gcaggggcag caggcgctgc tggcgtggtt taatgaaggt gatacccgtg
     1561 cctataaaat ccgcttcccg aacggcacgg tcgatgtgtt ccgcggctgg gtcagcagta
     1621 tcggtaaggc ggtgacggcg aaggaagtga tcacccgcac ggtgaaagtc accaacgtgg
     1681 gacgtccgtc gatggcagaa gatcgcagca cggtaacagc ggcaaccggc atgaccgtga
     1741 cgcctgccag cacctcggtg gtgaaagggc agagcacgac gctgaccgtg gcattccagc
     1801 cggaaggcgc aaccgacaag agcttccgtg cggtgtctgc ggataaaaca aaagccaccg
     1861 tgtcggtcag tggtatgacc atcaccgtga aaggtgttgc tgcaggcaag gtcaacattc
     1921 cggtcgtatc cggtaatggt gagtttgctg cggttgcaga aatcaacgtc accgccagtt
     1981 aatccggaga atcagcgatg ttcctgaaaa ccgaatcatt tgaacataac ggcgtgaccg
     2041 tcacgctttc tgaactgtca gccctgcagc gtattgagca tctcgccctg atgaaacggc
     2101 aggcagaaca ggcggagtca gacagcaacc ggaagtttac tgtggaagac gccatcagaa
     2161 ccggcgcttt tgtggtggcg atgtccctgt ggcataacca tccgaagaag acgcagatgc
     2221 cgtccatgaa tgaagccgtt aaacagattg agcaggaagt gcttaccacc tggcccgcgg
     2281 aggcaatttc tcatgctgaa aacgtggtgt accggctgtc cggtatgtat gagtttgtgg
     2341 tgaatgatgc ccctgaacag gcagaggacg ccgggcctgc agagcctgtt tctgcgggaa
     2401 agtgttcgac ggtgagctga gttttgccct gaaactggcg cgcgagatgg ggcgacccga
     2461 ctggcgtgcc atgcttgccg ggatgtcatc cacggagtat gccgactggc accgctttta
     2521 cagtacccat tattttcatg atgttctgct ggatatgcac ttttccgggc tgacgtacac
     2581 cgtgctcagc ctgtttttca gcgatccgga tatgcatccg ctggatttca gtctgctgaa
     2641 ccggcgtgag gctgacgaag agcctgaaga tgatgtgctg atgcagaaag cggcagggct
     2701 tgctggaggc gttcgttttg gcccggacgg gaatgaagtt atccccgctt ccccggatgt
     2761 ggcggacatg acggaggatg acgtaatgct gatgacagta tcagaaggga tcgcaggagg
     2821 agtccggtat ggctgaaccg gtaggcgatc tggtcgttga tttaagtctg gatgcggcca
     2881 gatttgacga gcagatggcc agagtcaggc gtcatttttc cggtacggaa agtgatgcga
     2941 aaaaaacagc ggcagtcgtt gaacagtcga tgagccggca ggcgctggct gcacagaaag
     3001 cgggaatttc cgtcgggcag tataaagccg ccatgcgtat gctgcctgcg cagttcactg
     3061 acgtggccac gcagcttgca ggtgggcaaa gtccgtggct gatcctgctg caacaggggg
     3121 ggtaggtgaa ggactccttc ggcgggatga tccccatgtt ccgggggctt gccggtgcga
     3181 tcaccctgcc gatggtgggg gccacctcgc tggcggtggc gaccggtgcg ctggcgtatg
     3241 cctggtatca gggcaactca accctgtccg atttcaacaa aacgctggtc ctttccggca
     3301 atcaggccgg gctgacggca gatcgtatgc tggtcctgtc cagagccggg caggcggcag
     3361 ggctgacgtt taaccagacc agcgagtcac tcagcgcact ggttaaggcg ggggtaagcg
     3421 gtgaggctca gattgcgtcc atcagccaga gtgtggcgcg tttctcctct gcatcaggcg
     3481 tggaggtgga caaggtcgct gaagccttcg ggaagctgac cactgacccg acgtcggggc
     3541 tgacagcgat ggcgcgccag ttccataacg tgacggcgga gcagattgcg tatgttgctc
     3601 agttgcagcg ttccggcgaa gaagccgggg cattgcaggc ggcgaacgag gccgcgacga
     3661 aagggtttga tgaccagacc cgccgcctga aagagaacat gggcacgctg gaaacctggg
     3721 cagacaggac agcacgggca ttcaaatcca tgtgggatgc ggtgctggat attggtcgtc
     3781 ctgataccgc gcaggagatg ctgattaagg cagaggccgc gtttaagaaa gcggacgata
     3841 tctggaatct gcgcaaggat gattattttg ttaacgatga agcgcgggcg cgttactggg
     3901 atgatcgtga aaaggcccgt cttgcgcttg aagccgcccg aaagaaggct gagcagcaga
     3961 gtcaacagga caaaaatgcg cagcaacaga gcgataccga agcgtcacgg ctgaaatata
     4021 ccgaagaggc gcagaaggct tacgaacgcc tgcagacgcc gctggagaaa tataccgccc
     4081 gtcaggaaga actgaataag gcactgaaag acgggaaaat cctgcaggca gattacaaca
     4141 cgctgatggc ggcggcgaaa aaggactatg aagcgacgct gaaaaagccg aaacagtccg
     4201 gcgtgaaggt gtctgcgggc gatcgtcagg aagacagtgc tcatgctgcc ctgctgacgc
     4261 ttcaggcaga actccggatg ctggagaagc atgccggagc gaatgagaaa atcagccagc
     4321 agcgccggga tttgtggaag gcagaaagtc agttcgcggt actggaggag gcggcacaac
     4381 gtcgccagct gtccgcacag gagaaatccc tgctggcgca taaagacgag acgctggagt
     4441 acaaacgcca gctggctgta cttggcgaca aggtcaccta tcaggagcac ctgaacgcgc
     4501 tggcacagca ggcggataaa ttcgcacagc aacaacgggc aaaacgggcc gccattgatg
     4561 caaaaaaccg ggggctgact gaccggcagg cagcgcggga agccacggaa cagcgcctga
     4621 aggaacagta tggcgataat cctctggcgc tgaataacgt catgtcagag cagaaaaaga
     4681 cctgggcagc tgaagaccag cttcgcggga gctggatggc aggcctcagg tcaggctgga
     4741 gtgagtggga agagagcgcc acggacagta tgtcgcaggt taaaagtgct gccacgcaga
     4801 cctttgatgg tattgcacag aatatggcgg cgatgctgac cggcagtgaa cagaactggc
     4861 gcagcttcac ccgttccgtg ctgtccatga tgacagaaat tctgcttaag caggcaatgg
     4921 tggggattgt cggaagtata ggcagcgcca ttggcggcgg cgcatcagcg tcaggcggta
     4981 cagccattca ggcagctgcg gcgaaattcc attttgcggc cggagggttt acgggaaccg
     5041 gcggcaaata tgagccagcg gggattgttc accgtggtga atttgtcttc acgaaggagg
     5101 caaccagccg gattggcgta ggaaatcttt accggctgat gcgcggctat gccaccggcg
     5161 gttatgtcgg tacaccgggc agcatggcgg acagccggtc gcaggcgtcc gggacgtttg
     5221 agcagaataa ccatgtggtg attaacaacg acggcacgaa cggtcagata gggccacagg
     5281 cactgaaggc ggtttatgac gtagcccgta aggcggcaat ggatgttgtg accgggcaga
     5341 tgcgcgatgg tggtctgttc tccggaggtg gacgatgaaa accttccgct ggaaagtgaa
     5401 acccggtatg gatgtggctt cggccccttc cgtaagaaag gtgcgctttg gtgatggcta
     5461 ttcccagcga gcgcctgccg ggctgaatgc cgacctgaaa acgtacagcg tgacgctttc
     5521 tgttccccgt tgggaggcca cggcgcttga gtcgtttctg gctgagcacg ggggctggaa
     5581 ggcctttctg tggacgccgc cttatgagtg gcggcagata aaggtgacct gcgcaaaatg
     5641 gtcgtcgcgg gtcagtatgc tgcgtgttga gttcagcgca gagtttgaac aggtggtgaa
     5701 ctgatgcagg atatccggca ggaaacactg aatgaatgca cccgtgcgga gcagtcggcc
     5761 agcgtggtgc tctgggaaat cgatctgaca gaggtcggtg gagaacgtta ttttttctgt
     5821 aatgagcaga acgaaaaagg tgagccggtc acctggcagg ggcgacagta tcagccgtat
     5881 cccattcagg ggagtggttt tgaactgaat ggcaaaggca ccagtacgcg ccccacgctg
     5941 acggtttcta acctgtacgg tatggtcacc gggatggcgg aagatctgca gagtctggtc
     6001 ggcggaacgg tggtccggcg taaggtttac gcccgttttc tggatgcggt gaacttcgtc
     6061 aacggaaaca g
//
LOCUS       DAZKFZ010000245         6049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000245 DAZKFZ010000000
VERSION     DAZKFZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.245"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2390)
                     /locus_tag="ACHOQW_004274"
     CDS             complement(<1..2390)
                     /locus_tag="ACHOQW_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9575230.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTALGELKT
                     LTEMQKQELLRNATLIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERA"
     gene            complement(2438..2647)
                     /locus_tag="ACHOQW_004275"
     CDS             complement(2438..2647)
                     /locus_tag="ACHOQW_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP9575231.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(2743..3117)
                     /locus_tag="ACHOQW_004276"
     CDS             complement(2743..3117)
                     /locus_tag="ACHOQW_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9575232.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(3132..3848)
                     /locus_tag="ACHOQW_004277"
     CDS             complement(3132..3848)
                     /locus_tag="ACHOQW_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9575233.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(3914..4258)
                     /locus_tag="ACHOQW_004278"
     CDS             complement(3914..4258)
                     /locus_tag="ACHOQW_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP9575234.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(4255..4701)
                     /locus_tag="ACHOQW_004279"
     CDS             complement(4255..4701)
                     /locus_tag="ACHOQW_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9575235.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4698..5048)
                     /locus_tag="ACHOQW_004280"
     CDS             complement(4698..5048)
                     /locus_tag="ACHOQW_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9575236.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(5058..5384)
                     /locus_tag="ACHOQW_004281"
     CDS             complement(5058..5384)
                     /locus_tag="ACHOQW_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9575237.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5387..>6049)
                     /locus_tag="ACHOQW_004282"
     CDS             complement(5387..>6049)
                     /locus_tag="ACHOQW_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9575238.1"
                     /translation="DGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GG"
BASE COUNT         1217 a         2027 c         1447 g         1358 t
ORIGIN      
        1 ctggctcgct ccccctgccc gtaccccagc agttcagcat cattcgacgc acgcgcagcc
       61 gcattctcat tcttcagtgt ctcttcccgg gatcgcaact gttcccggat tttttgctgg
      121 tcaatcaggg tcgcgttacg cagcagttcc tgcttctgca tctccgtcag ggttttcagt
      181 tcacccagcg ctgtctggta tttcagcttc gccagctctg tattctgccc caccagtgcc
      241 agttgctctt tctgctgctt cagcagccgg gaaaaactgt cttccgcttt ttccgtctct
      301 gattttccac cccgggattt gggtttattc gcctcgttat tgcgccaggc ttccagggca
      361 ttactgatat aacgttgtct cgcctcctga tacggatcac ccacaaaacc gaggtcatcc
      421 gccgcatacc ccagtcggac acgctctttt tcttcccctt tcagtctgga cagggccagc
      481 tcacgctctg tttttgtcag ggcactctgc tgtttatcat ccagggtggc ctgcggcagc
      541 cgtaacggta cattcaccag tccctgacgc tgctgaagca gttcattccc cagccccagc
      601 agacggttga attccgtatg ctgaccgttc ataaccagca tggactggta caccttattc
      661 tgctctgccg cctgctgacg aattaacgcc acacgacggt cttccagccc ggcaagcaca
      721 tcctgaatgg actgcgcttt ttcctgcatc tgtgccagac gggactgctc aacggcaagc
      781 tgctctgttg cctgagaaag cccttccgtt acggtcttca ccgatgtcag atggtttatc
      841 atgaatccgt caccggtcgt ccagcccggg ttcgccagaa catactgata tcctgcgatt
      901 ttttcctgca gggatttcac ccgactggcc tgttcatcaa tcagccggtt ctgctctgtc
      961 agcgccgccc gtgttcgtcc ttcattatct gaggcttcag gcaaagacat tgacggcgtt
     1021 ttatgcgcga tttcatctat cgtcagtgca tactggcgcg cagactccct ggcctgctcc
     1081 tgattctggt acagcgtgta ccatgctgca gcccccagca tcaccagtcc gggtacgcca
     1141 ccaaccagcc ccagcgcacc gctcatcaga cgtgagccca ccgccgttgt actgttcagc
     1201 gcattctggg cggcgcttct ggcagcaata tttctgttca ggcgttcctg tgtggccgcc
     1261 agacgggcct ctgcagcaat ctgcatctcc gtcccgcggg ctgccgccac ggcctgctga
     1321 gcacggtaca cggctgccct tgcccgcgcc gtggcaatct gcgttcccct gaactgtgct
     1381 tccgccagtg caacttcatt acgtgcagcc gtcacaagtc ctgccgtggc agacatcgct
     1441 ccggaggcca tattgccaaa gtaccgggca accccgacgg caaccagcgc gcccacggct
     1501 gttgccacat tatcaatctg tccggcaaca ccgttcagca tgccggagag cgtttttgtc
     1561 accccgctgg cctcattcgc accgcccacc caggccataa aggcgttttc cacctttgtg
     1621 atactactgg aaaccgtttc cggcatggcc gcatattcat cacgtaatat ccccagctgg
     1681 ctgattaacg cggggaccac tttatccgct gtcagttttc cgtcatccgc cattgccttc
     1741 agatctttac gggccacgcc catacccgca gccagtgcac gtacgatccg gtcaccactt
     1801 tcattgaccg aattaaactc ctcaccacgc aatacaccct gcgccagcgc ctgactgaac
     1861 tgggtgatca ccgagcccgc ctctgccgta ctggcaccgg agattttcag ccctgtcgaa
     1921 atggcctccg tcaccttcag cacatcatca gcactgtaac catattcacg catcgaggca
     1981 gccgaacggg caaacagggc cgcattatcc gaaaatgcgg tgcctgtccg ctggctgata
     2041 tccatcagca ctttctgtga tgacgcaaat tcatccgatg actgcgacgc ctgtttcaga
     2101 cgggcattca cggagctcca ttcatccgcc agcgaaatca ggtgtccggt ggcaaaggca
     2161 ccggcaaacg caccggtcat tccgacagcc gaagcgcgga tttccgtcaa ctggctgtgc
     2221 agctcagcca gagcccggcg ctgctccctg gctgccgcag cagcctgacg tccgccattc
     2281 tgcagggtcc ggtaatattc actgcccata cgggacgccc gctggatctc cgactggaat
     2341 gactgtgaat ttgccgaaat tttgataatc agttcacgta acgtcgccat tcacctttct
     2401 ccgggcgtaa aaaaaccgcc tcagcggttc tcatcattca tgactgtgct gcaaggctca
     2461 gcgcgtcttc cagcgccgca aacggatcca cctccggctt atcctcatcc tcgccccagc
     2521 agagcatggc gtccttcagt gcaacattca tcccctgtgc cccgaaaacc gctttcacga
     2581 tctgtgcatt acggatatcc ccgcgctcat cacccagcgg ggatatcctg tcgaactcca
     2641 tccacatcat cgcctcgctc gcactcaggc tgtggcgcag ttcggataag gtgcgcccca
     2701 gacggagcgc aagtcgcatc agaaagcgaa tttccgggcg ggctactttt ttctggccga
     2761 ctctgcatca gcgatcagtt ccagtgcctg acgcagcaac cgggcatgta ccggaccata
     2821 gacggccagc acctgctcac ggtcgtccgg agtgaacacc cgctgcaggt ccgtatcaca
     2881 caggacatcg cagaacagcg tcacatccgc ttccaggtta cggcgggttt tcgccaccac
     2941 cgaaagggta tcgtcatcct ctccatcacc attgagcact tcctgccaca gataccaggc
     3001 ctctgccgaa ggctcccgca gcaccacgct gacattaccc cattccggca ccttcaccgt
     3061 tttatgacga aaccctgaca gtctggccag cgccagcgtt ttcagatcct ttttcatgat
     3121 gacccatccc cttatccggc ggctgcgctc actgtcacgg tgcattcaac agacgtcaca
     3181 ctctgtgctt tctctgccga atcggtcacc acacaggtat atttccccgc atcagcggac
     3241 tgcgcacctg gcttactgaa ggtgtctgtc gtctgcccgt caaccggctg accatccttc
     3301 ttccaggcgt atttatacgg cggcgttccc ccgttggcac tgactgacat tgtcagcagc
     3361 gcaccggtat tcacggtaag tgtcttatcc agatttttca caaacgccag cggtaccaca
     3421 aaggacaccg gtttgccttt cagacgcagt gagaacgttg cagccaccac gccgttggta
     3481 ccggatgacc aggtgtgctg gcgcacttcc gccaggaact taaagccctt accggacgga
     3541 aacagcacct taaacgcata caacgcgtca ttgtcatagg catcacgcag ggcgttctgg
     3601 gcctgattca gataaaaatt gcccgacatg gaaatctcag acgacgcccc cagaccgttg
     3661 atgttctcct gctctgtgga gcagagcgtg gtcacatcaa tatcctgttt ctgcccggcg
     3721 gtgaactgca cttccttgat ggtgcagtcc aggcgcagat attccgcctt atccatagtt
     3781 tcagcagtcg ccggggcaga tgaaatcatc acctgcgtca gctgtgagcg ttcatacaaa
     3841 gcagacattc tgcctcctga taataaaaaa cccgcacgcg gcggggtatg ggttttgtag
     3901 aaaaaagaaa aagtcacacc gtgacctgaa actccagggt tgcacggtaa cagcggtttt
     3961 ccggaatata gtcctgcatt tcactgacgg atcccggggc cagcagcatt atggcttcac
     4021 gggcgtcctg acgtatctga cgcgcctgcg tcacagtccc ggcataaacg tctatctgca
     4081 ccgacactga ggactccgcc tgcccgccca tcacgtccgc tgacaccgat gaaatcaggc
     4141 tgaaaaccac ccacggaagc gccaccgacg gcctgccatc cagcaggggg accacatacg
     4201 ggtacacctg cccgccggca agatgcgcca gatgaggata caaatccgcc tccgtcatcg
     4261 tctcagtacc tcatcaatgg cccggttcat ccgcgcaatc gccacctgtg ccgcctgttc
     4321 actgcgcaca tcaaatgccg ggcgcacaaa cgggtgcggt ggcatattca cggtccccat
     4381 ttccacaaac cgccagtaga aagcgttgcg cgggttatcc gccttcatgg tgttatcgct
     4441 gttaccggtg tccggattaa caccccggat atgcacaccg gattccatcc cgccatcgcg
     4501 ggagcgccgg gaaaggacca ccacattgcg gcgcagtttt cccctgcgta ccggtgcccg
     4561 tgacaccact tcttctttca gcacattcgc acccgcacgg gttgcctcac gcagcacccg
     4621 gttattttct gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa
     4681 atccagcagg gtttcgatca tttttcccct cccagccgac agagaatttc cagacgtccg
     4741 ccggtcgcat ccggcacggg cagcccgaca acgttcagga tccggtcacg ccagggacca
     4801 ctcagcacat gaagtcgtga cgctgccgtg atttcccgac cggactgacc gcgcacccag
     4861 atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg gctgctcctg
     4921 ccacggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat ttctccggca
     4981 gcattacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg acctgcagat
     5041 attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat cagcacggaa
     5101 acagaaaacg gcagctcccc ctgaatcagt tcttcccgct ccgcaagatc cggattccgg
     5161 tacagcatcc cggtcagtcg catggcagcc cccttcatcc gggttaatgc ctcgcccggg
     5221 atcagttcac cgtcctcacg aatcacctta tcccggctgc cctgaatgta ggccagcagc
     5281 acggcggtag cctgacgaac cttgtccatc agcatgtcat catccgcgtc atggtcgaca
     5341 cgcagatgtg ccttgatctc ttccagtgtc agtaatgccg tcattttcag cctcctgcat
     5401 cccgcccacg ttttgcagcc agggtccagg ctgatgaatg agcttctccg ggtttatctt
     5461 cggtcatact gttgcagtgc cacagcgagc ccccccacgt caccgtatcg ccggggtggt
     5521 aggtttcacc ggctctgaac acaccgcggt agagcatcac cggcagggaa aatgtttttt
     5581 ccgtacgctg gccactgctc tgccggacca ccacagagaa caaccgctca cccgtcatgc
     5641 tgacgtcaat atccgccacc ccgtcaacca ggcattccca tccccgcatc ccgtgcgttt
     5701 tttcatacgc ccgccagagt ccgccctggt gtgtggcata cgtgccccgg ggaaaggatt
     5761 tttgatcgtc aatggcgggg agtatttcca gagccgtggc atcacgcccg tcctgcggag
     5821 ccggcagggc actcaccgca tccagaaccg ccttctgcag aacatccgga tcgtagtcac
     5881 gaccatcacg cggaacagga atatggctta ccgcctcctt caccatctgt tcaagcatcg
     5941 gacgcacatc atccggggtg agacttttac cgtctgccgg ctgcggaata tttgcgaccg
     6001 catcattcac cgccttctgc agtacttccg gatcatagtc acgaccgtc
//
LOCUS       DAZKFZ010000246         6028 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000246 DAZKFZ010000000
VERSION     DAZKFZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(135..1316)
                     /locus_tag="ACHOQW_004283"
     CDS             complement(135..1316)
                     /locus_tag="ACHOQW_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9575239.1"
                     /translation="MPLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRF
                     AGKEKKLVIGVYPGISLALARQKRDEARKLLAEGKDPSYEKQASKHALRESIACTFCT
                     LAEELFTHMACRWTAGYLADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGK
                     LEALRKTRQLCSQIFDYAIATGRATINPASTLNSVVLHPEKQQRRMLSEEQLGAFMYQ
                     MKVSGNFMSLVTQILVLTAVRTGELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLS
                     HQTQMLLKEIKIITAPRNSELVIPGVYRDNQPRGRGCINEFLQQVGWHTFTTAHGFRH
                     TFSTILHDHDFSSAWIELQLAHVDKNIMRGTYNHAQYLDGRREMMQWYADYIDSLSEQ
                     A"
     gene            1491..2306
                     /gene="ulaE"
                     /locus_tag="ACHOQW_004284"
     CDS             1491..2306
                     /gene="ulaE"
                     /locus_tag="ACHOQW_004284"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP9575240.1"
                     /translation="MLPAGECWLERLQLAKTLGFDFVEMSVDETDERLSRLDWSREQR
                     LALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKAIQFAQDVGIRVIQ
                     LAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYPLMNSISKALGYAH
                     YLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPGVFKNVPFGEGVVD
                     FERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMAKAGMVEAA"
     gene            2306..2992
                     /gene="ulaF"
                     /locus_tag="ACHOQW_004285"
     CDS             2306..2992
                     /gene="ulaF"
                     /locus_tag="ACHOQW_004285"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP9575241.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3121..3396)
                     /gene="yjfY"
                     /locus_tag="ACHOQW_004286"
     CDS             complement(3121..3396)
                     /gene="yjfY"
                     /locus_tag="ACHOQW_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP9575242.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            3723..4118
                     /gene="rpsF"
                     /locus_tag="ACHOQW_004287"
     CDS             3723..4118
                     /gene="rpsF"
                     /locus_tag="ACHOQW_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9575243.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4125..4439
                     /gene="priB"
                     /locus_tag="ACHOQW_004288"
     CDS             4125..4439
                     /gene="priB"
                     /locus_tag="ACHOQW_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP9575244.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            4444..4671
                     /gene="rpsR"
                     /locus_tag="ACHOQW_004289"
     CDS             4444..4671
                     /gene="rpsR"
                     /locus_tag="ACHOQW_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9575245.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            4713..5162
                     /gene="rplI"
                     /locus_tag="ACHOQW_004290"
     CDS             4713..5162
                     /gene="rplI"
                     /locus_tag="ACHOQW_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9575246.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(5233..6027)
                     /gene="yjfZ"
                     /locus_tag="ACHOQW_004291"
     CDS             complement(5233..6027)
                     /gene="yjfZ"
                     /locus_tag="ACHOQW_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9575247.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1561 a         1387 c         1546 g         1534 t
ORIGIN      
        1 ctggtgttca gattattgtc tgcaggggca gttcagaaga tgtgagcaaa taaaaaagaa
       61 aacataaata aaaccatccg gcctccaaag ccggatggtt attcattatt tctgcatcac
      121 tgaactttca ttcatcaggc ctgctcagaa agtgaatcaa tgtagtcagc gtaccattgc
      181 atcatttcac gacgaccatc gagatactgt gcgtggttgt aggtgccgcg catgatgttt
      241 ttatcaacat gggcaagctg gagttcaatc caggcagagc tgaaatcatg gtcatggaga
      301 atggtactga aagtatgccg aaaaccatga gctgtagtga atgtatgcca gccaacttgt
      361 tgaagaaact cgttgatgca accgcgacca cgaggttgat tatctctgta tacaccagga
      421 ataaccagct cggagttacg gggagctgtg attattttta tctctttcaa taacatttga
      481 gtctgatgag aaagtggaac aacatgcggc cttcgcattt tcattctctc ttttgggatt
      541 acccataagg cattggtaaa atcaatttca tcccaagtag caaggcgtaa ttctcccgtt
      601 ctaacagcag ttagaaccag tatttgcgtt accagagaca taaaattgcc tgaaactttc
      661 atttggtaca tgaaagcgcc taactgttct tcagatagca tccgtctctg ctgtttttct
      721 ggatgaagaa caacactgtt gagagtcgat gctgggttaa tagtggcgcg ccccgtcgca
      781 atggcgtaat caaatatttg agaacagagc tgacgagtct tacgtaacgc ttcaagtttg
      841 ccattctttt ctataaccct cagtgcattc aatatttcca aggggcgaat atcagccaca
      901 ggacgtttcc ctagcatggg attaatatga ttctcgaatc ttgcacgctg gtccgctaaa
      961 taacccgcag tccaacgaca agccatatgt gtgaacaact cttctgcaag agtacaaaat
     1021 gtgcatgcaa tactttctct tagtgcatgt ttactcgcct gtttttcata tgaaggatct
     1081 tttccttcag cgaggagttt acgtgcttca tcccgtttct gacgcgccag agccagtgat
     1141 atacctggat agacaccaat caccaatttc ttttctttac ccgcaaatcg atacttcaga
     1201 ttccagtaac gagcgccaga tttcttgata agaaggtaaa gtccttgacc atcagaaagt
     1261 ttgtaatcct tgtcagcagg tttggccgac tcaatttgcc gaacagtaag tggcatgatg
     1321 ttaactccga aaagtagccc ccagagtagc ccccaaaatt atattgatga tcatggagga
     1381 tggtttacgt ccagagtaaa agaatttatg ataatacctt attttaaagt gagttaaagg
     1441 gggtggagag agttctgttg aaatcagtgg aaataaacag ggcttaataa atcctccccg
     1501 ccggggagtg ctggctggaa cgcctgcaac tggcaaaaac gttgggcttc gattttgtcg
     1561 agatgtcggt agatgaaacg gacgagcgcc tgtcgcgcct cgactggagc cgcgagcagc
     1621 gtctggcgct ggtgaatgcg attgttgaaa ccggcgtgcg cgtgccgtcc atgtgccttt
     1681 ctgctcatcg tcgtttcccg ctgggcagtg aagatgacgc ggtgcgggcg caggggctgg
     1741 agattatgcg taaagctatc cagttcgccc aggacgttgg tattcgcgtg atccaactgg
     1801 cgggctatga cgtttactat caggaagcca ataacgaaac gcgtcgtcgt ttccgtgacg
     1861 gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt gacgctggcg atggagatca
     1921 tggattatcc gttgatgaac tccatcagca aggcgctggg ctacgcgcac tatctcaaca
     1981 atccgtggtt ccagctctac ccggatatcg gcaacctgtc ggcgtgggac aacgatgtgc
     2041 agatggagtt gcaggccgga atcgggcata tcgtcgcggt acatgtgaaa gacaccaaac
     2101 cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt ggatttcgaa cgttgcttcg
     2161 aaacgctcaa acagagtggc tattgcgggc cgtacctgat tgagatgtgg agcgaaacgg
     2221 cggaagaccc ggcggcagaa gtggcgaaag cgcgtgattg ggtgaaagcg cgcatggcga
     2281 aagcgggcat ggtggaggcg gcataatgca aaagctaaaa cagcaggtgt ttgaagccaa
     2341 catggatctg ccgcgctatg ggctggtgac ctttacctgg ggcaacgtca gcgctatcga
     2401 ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtc gcctacgaaa ccatgaaagc
     2461 agacgatatg gtggtggtcg atatgagcgg taaggtggtg gaaggggaat atcgtccgtc
     2521 ttccgacacc gcgacgcatc tcgaactcta ccgtcgttat ccgtcgcttg gcggcattgt
     2581 ccatacccac tccactcatg ctaccgcatg ggcgcaggcg gggctggcga tcccggcgtt
     2641 aggcaccacg cacgctgact acttctttgg tgatatcccg tgtacgcgtg gattaagcga
     2701 agaagaggtg cagggtgagt atgaactgaa cactggcaaa gtgattatcg aaacgctggg
     2761 caacgccgag ccgctgcata cgccgggaat tgtggtgtat cagcacggac cgttcgcctg
     2821 ggggaaagat gcccacgacg cggtgcataa cgcggtagtg atggaagaag tggcgaaaat
     2881 ggcgtggatt gcgcgcagta ttaatccaca actcaatcac atcgacagct tcctgatgaa
     2941 taaacacttc atgcgtaagc acggccccaa cgcttattac gggcagaagt agaacacgcg
     3001 ctgcggaaat ttccttcctc gggagataac tggtctaatt ccgcagccgt ttttcaaaaa
     3061 aagccccctg cgaagggggc aaagcaaact atggcaatgt ttcgttggtt atacctggtg
     3121 ctagcgataa atatccgcgc tggcgtgcat attgccgtta ctccccggtt cccgcatcag
     3181 aattacgtgg tagtacgttg cgccttgcgc atctgtttct tcatttaatg cctgacgtgc
     3241 ttcgctttca gtggcgaaat tatgattgat ataaatcacg cctaagcttt gcacatcgtc
     3301 catatttctg gcctggtggt tattaatttc aatggctgcc catgtatttg cacttagcaa
     3361 aagcacagcc agaagggcta aaactcgact gaacatagat acctcctcga cggctgactt
     3421 tgtgtgctct cctctgtgat gatcttctga tttaatttta atcaatgata aagaagttga
     3481 tggtgaccat ttctgatgca gttgttcgaa aaaacaccat gatgaagtgt gatgaacttc
     3541 aaatcagcgt gttagtccac tttggtgaaa gatgagattt atttgtcggc tggccttgag
     3601 tttagcgggg tatgtcagta ctataacgcg tcatttttca gccgaccttt aacacgttcc
     3661 ttgcctcccc gggattcggc tgacccagac aggaggctga ataatccgta aggagcaatt
     3721 cgatgcgtca ttacgaaatc gtttttatgg tccatcctga ccagagcgaa caggttccgg
     3781 gcatgatcga gcgctacact gctgccatca ctggtgcaga aggcaagatc caccgtctgg
     3841 aagactgggg ccgccgtcag ctggcttacc cgatcaacaa actgcacaaa gctcactacg
     3901 ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga gctggaaact accttccgct
     3961 tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa gcacgctgtt accgaagcat
     4021 ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg cgatgatttc gcaaacgaaa
     4081 ccgctgatga tgctgaagct ggggattctg aagagtaatt tctgatgacc aaccgtctgg
     4141 tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt cagtccatca ggaattcctc
     4201 actgccagtt cgtgcttgag catcgttctg tgcaggagga agccggcttt caccggcagg
     4261 catggtgtca aatgcccgtt attgttagcg gacacgaaaa ccaggccatt actcacagta
     4321 taacggtcgg cagtcgcata accgttcagg ggttcatttc atgccacaag gcaaagaacg
     4381 gactgagcaa aatggttttg catgccgagc agattgaatt gatagattct ggagactagc
     4441 catatggcac gttatttccg tcgtcgcaag ttctgccgtt tcaccgcgga aggcgttcaa
     4501 gagatcgact ataaagatat cgctacgctg aaaaactaca tcaccgaaag cggtaagatt
     4561 gtcccaagcc gtatcaccgg tacccgtgca aaataccagc gtcagctggc tcgcgctatc
     4621 aaacgcgctc gctacctgtc cctgctgccg tacactgatc gccatcagta atcggtcacg
     4681 gtccattaat acgactttga gaggataagg taatgcaagt tattctgctt gataaagtag
     4741 caaacctggg tagcctgggt gatcaggtaa acgttaaagc gggctatgct cgtaacttcc
     4801 tggtaccaca gggtaaagct gttccagcta ccaagaaaaa cattgaattc ttcgaagcac
     4861 gtcgcgctga actggaagct aaactggctg aagttctggc agctgctaat gctcgcgctg
     4921 agaaaatcaa tgcactggaa actgttacca tcgcgtctaa agctggcgac gaaggtaaac
     4981 tgttcggttc catcggtact cgcgacatcg ctgacgctgt aactgcagct ggcgttgaag
     5041 tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg taccactggc gaacacgaag
     5101 tgagcttcca ggttcacagc gaagtattcg cgaaagtgat cgtaaacgta gtagctgaat
     5161 aattcgttat tcaacgagac gtaaaaacgc cgaccactgg tcggcgtttt gctttctatt
     5221 tttcgtcagg tattagtttc gcaagtagat ccattcacca ttctcttgtg tccataagtc
     5281 aggatggttt gctggcgtta gatacatacg agcgcaactg atgtctcgct cctgcggcac
     5341 tcgctcgaaa cccaaacccg agtagattgc ggctgaactt gataaggaac gcagaccaat
     5401 tttcaaatca tggggtaaat cagaatacgc gtttagcaat gcagcaatga tgaatccgag
     5461 ggcattagaa acgcctgtaa cagtgcgaag ttggtcgata taaagatcga ttgttggtag
     5521 ttcagatgac cagcaattag gcatttgggc aggaagggtt acaacgtatc ctatcgtttg
     5581 gccggtatag tctggagtac cataaccagg tcccaaaacc acttcgtgta ttgtgtattt
     5641 actgttgggt tcgaaggatt cgacatcttg caataaatat ttcagttctg cggttaacct
     5701 cgcacccgcg taagctttat tgtatttctg ggcgtgaata ttatctaatg tagcttcatg
     5761 gttccatcca aaagaattat caccattcat tgttactcca tcgtaagcat tacacttcgt
     5821 tgctaactta ttgaatgctt gccagtctga gagatctagt ttgtggatct caacagtacg
     5881 tctgccatcg caattatgat ctcttccacg atttgaaagt aatgtagcgc gtgcgtattg
     5941 atgaaatttt aattttactg gtttgtcggt ggacgaaaaa cgagataaat atgcaggaaa
     6001 tgaataaata gtggttggta atgtcatt
//
LOCUS       DAZKFZ010000247         6023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000247 DAZKFZ010000000
VERSION     DAZKFZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            219..665
                     /locus_tag="ACHOQW_004292"
     CDS             219..665
                     /locus_tag="ACHOQW_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP9575248.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            676..2115
                     /locus_tag="ACHOQW_004293"
     CDS             676..2115
                     /locus_tag="ACHOQW_004293"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP9575249.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2105..3175
                     /locus_tag="ACHOQW_004294"
     CDS             2105..3175
                     /locus_tag="ACHOQW_004294"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9575250.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3175..4923
                     /locus_tag="ACHOQW_004295"
     CDS             3175..4923
                     /locus_tag="ACHOQW_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP9575251.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4973..>6023)
                     /locus_tag="ACHOQW_004296"
     CDS             complement(4973..>6023)
                     /locus_tag="ACHOQW_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP9575252.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAPEA
                     KPTEAPVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLK
                     DFKQGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVAL
                     GNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQL
                     AELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKE
                     ADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRA
                     LREYNEMVDDYNSLR"
BASE COUNT         1422 a         1525 c         1501 g         1575 t
ORIGIN      
        1 ttagacaaat tatctgagac atttaaacat tcacaaaatc attaaataat aactctttat
       61 tcacaagatt tattttacct actaatatgt gccttacgcc gcaaaattta tccttcactc
      121 tgctttatat tgtcacgact tcacgacata tccgcgctca tcacaaaccc gccccgctat
      181 tacaattaac tgaaatattt tttcatggag tccgattaat ggcagctctt actgcaagct
      241 gtattgacct gaatattcag ggcaatggcg cttattccgt tctgaagcag ttggcgacaa
      301 tggcgttaca aaacggtttt atcaccgact cacaccagtt cctgcaaacc ctgctgctgc
      361 gcgaaaaaat gcactccacc ggttttggtt ctggtgtcgc cgtgccgcac ggtaaaagca
      421 cctgcgttaa acaaccgttc gtgttattcg cccgcaaagc gcaggctatt gactggaaag
      481 ccagcgacgg cgaagacgtc aattgctgga tctgcctcgg cgtaccgcaa agtggcgaag
      541 aggatcaggt caaaatcatc ggcacactgt gtcgcaaaat tattcaccag gaatttattc
      601 atcaactgca acagggcgat accgaccagg tgcttgcctt gttaaatcaa accctcagct
      661 cataaggaag tggcgatgga gtcatcctta cgtattgtcg cgatcaccaa ctgccccgcc
      721 gggatcgctc acacctacat ggtggcggaa gccctggaac agaaagcccg ttctctcggc
      781 cataccatta aagtggaaac tcaagggtcc agcggcgttg aaaaccgctt atccagcgaa
      841 gagattgccg ctgccgatta cgtgattctc gccaccgggc gtggcctgag cggtgacgat
      901 cgcgcacggt ttgccgggaa aaaagtttat gagattgcca tctcccaggc gctgaaaaat
      961 atcgatcaga ttttcagtga attaccgaca aattcgcagc tttttgccgc agatagcggc
     1021 gtgaagctag gcaaacagga agtacaaagc ggcagcgtga tgagccacct gatggcgggc
     1081 gtctctgcgg cgctgccgtt tgtcatcggc ggtggtatcc tggtggcgct tgccaacatg
     1141 ctggtgcagt tcggtttgcc gtataccgat atgtcgaaag gcgcgccgtc gtttacctgg
     1201 gtagttgaat ccatcggtta ccttggcttc acctttatga tccccatcat gggcgcttat
     1261 attgcctcgt cgattgccga taaaccggct ttcgctccgg cgtttctggt ttgctatctg
     1321 gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg gattcctcgg cgcggtggtg
     1381 ctggggctgg cgattggcta tttcgtcttc tggttccgta aagtccgtct tggcaaagca
     1441 ttacaaccgc tgctcggctc catgcttatc ccattcgtta ctctgctggt ttttggcgtg
     1501 ctcacttact acgttatcgg gccggtgatg tccgacctca tgggcgggct actgcacttc
     1561 ctgaatacca ttcctccatc aatgaagttt gcggcagcgt ttctggtcgg tgcgatgctg
     1621 gcgttcgata tgggcggccc catcaataaa accgcctggt tcttctgctt ctcactgctg
     1681 gaaaaacaca tttatgactg gtacgccatc gtcggcgttg tccccgtcgc ggcgggtctt
     1741 gcgacgttca ttgcgcccaa actgtttacc cgacaggaaa aagaggctgc cagtagtgcc
     1801 attgtggtcg gtgccacagt cgcaaccgaa ccggctattc cttacgctct ggcagctccg
     1861 ttgccgatga ttaccgccaa tacgctggcg ggcggcatta ccggcgtgct ggtgatcgct
     1921 ttcggaatca aacgtctggc acctggtttg ggtatctttg acccgctgat cggcctgatg
     1981 tcgccggtag gttcgtttta tctggtgctg gcgattggcc tcgcgctgaa catttcattc
     2041 attatcgtcc tgaaaggatt gtggttacgc cgcaaagcga aagccgcgca acaggagctt
     2101 gtccatgaac attgagttac tgcaacagtt gtgcgaagcc agcgccgtca gcggcgatga
     2161 acaggaagtt cgcgacattc tgataaacac gctggaacct tgcgttaatg agatcacctt
     2221 tgatggtctg ggcagctttg ttgcccgtaa gggaaataaa ggtccaaaag ttgccgttgt
     2281 cgggcatatg gatgaagtcg gctttatggt cacccacatc gacgagagcg gttttctgcg
     2341 ctttaccacc attggcggct ggtggaatca gtcgatgctc aaccaccggg taacaatacg
     2401 cacacacaag ggagtgaaaa tccctggtgt gattggttcc gtagcgcctc atgcgttaac
     2461 ggaaaagcaa aagcaacaac cgctgtcatt tgatgagatg ttcattgata ttggcgcgaa
     2521 cagtcgcgaa gaagcgaaaa aacgcggtgc ggaaattggc gattttatta gcccggaagc
     2581 caattttgcc tgctggggcg aagataaaat agtcggcaag gcgctggata atcgcatcgg
     2641 ctgcgcgatg atggctgagc tactacagac agtaaataac ccaggaatta cgctgtacgg
     2701 cgtcggcagc gtggaagaag aagttgggct acgcggggca caaacctcgg ctgaacacac
     2761 taaaccggat gtggtgatcg tgctggatac cgccgtcgcg ggcgatgttc cgggcattga
     2821 taacattaaa tacccgctga aactgggcaa cgggccgggg ctgatgctgt ttgacaagcg
     2881 ctacttaccc aaccagaaac tggtggcggc gttaaaaaac tgtgccgcac ataacggttt
     2941 accgctgcaa ttttccacca tgaaaaccgg agcgacggat ggcgggcgct acaacgtaat
     3001 gggcggaggg cgtccggttg tcgcgctgtg tctgccaact cgttatctgc acgctaacag
     3061 cggtatgatt tcaaaagccg attatgatgc tctgctcacg ctgatacggg attttctgac
     3121 gaccttaact gcggagaaag tcaacgcgtt tagccagttc cgtcaggtgg attaatgcta
     3181 aacgaacgcc agttaaagat tgtcgatctg ctggagcaac agccgcgcac gcctggcgag
     3241 ctggcgcaac agactggcgt ttcaggcagg accatcctgc gtgatattga ctatctcaac
     3301 tttaccctta acggcaaagc tcgtatctct gccagtggca gtgcgggcta tcagttggaa
     3361 atcttcgagc gccgcagctt ttttcagttg ctgcaaaagc acgataacga cgatcggctg
     3421 ctggcgctgt tattactgaa tacctttact ccccgtgcgc aactcgcctc ggcgcttaat
     3481 ttgccagaaa cttgggtcgc agagcgtctg ccccggttaa aacagcgtta tgaacgcact
     3541 tgttgcctgg ccagtcgtcc cggtttgggc catttcattg atgaaacaga agagaaacgc
     3601 gttatcttgc tggcgaactt gctacgcaaa gatccgtttt taataccttt ggctggcgta
     3661 acacgagaca accttcagca tttatccacg gcctgcgaca accaacaccg ctggccgctc
     3721 atgcaggggg attatctctc cagcctgatt ctggcgattt acgccctgcg taatcaactg
     3781 accgatgagt ggccgcaata tcccggtgac gagataaaac aaatcgttga acataacggt
     3841 ctgtttcttg gtgataacgc tgtaagaacc ctgacaggtt tgatagagaa acagcatcag
     3901 caagcgcagg taatttctgc cgatcatgtg ctggggttgc tgcaaagggt gccaggtctc
     3961 gcgtcattga atattattga tacgcagctg gttgagaaca ttaccggtca tttattacgt
     4021 tgccttgccg caccagtgtg gattgctgag catcgccaaa gcagcatgaa taacctgaaa
     4081 gccgcctggc ctgcggcctt tgatatgagt ctgcacttta ttacgctact gcgtgaacag
     4141 ctcgatattc cccttttcga cagcgatctg atcggtttgt attttgcctg tgcgctggaa
     4201 cgacatcaaa acgaacgcca gccgatcatt ttgctctcgg accagaacgc gattgccact
     4261 attaatcagc tcgccattga gcgcgatgtt ttacattgtc gggtgattat tgcccgtagc
     4321 ttaagcgaac ttgttgccat tcgcgaagag attgagccgt tattgatcat taacaacagc
     4381 cattatttac tggatgacgc ggtaaataat tacatcaccg taaaaaatat cattacggct
     4441 gccggtatcg aacaaataaa acattttctg gcgacggcat ttattcgcca gcaaccggag
     4501 cgttttttct ctgcccccgg aagttttcat tattcgaatg tacgcggtga aagctggcaa
     4561 catattaccc ggcaaatttg tgcgcaatta gtcgcacaac accatattac cgccgatgaa
     4621 gcgcaacgga tcatcgcccg cgaaggcgaa ggtgaaaacc tgattgttaa tcgcctcgcc
     4681 atcccacatt gctggagcga acaggagcga cgttttcgtg gattttttat taccctcgcc
     4741 caaccagttg aggtgaataa cgaagtcatt aaccatgtct tgatcgcctg cgccgccgcc
     4801 gatgcgcgtc atgagctaaa aatatttagc tatctggcaa gcgtattgtg tcagcatccg
     4861 gcagaagtta ttgccgggct aacaggatat gaggcattta tggagttact tcacaagggg
     4921 tgaactgtgt cggatgcgcc gcatccgaca ctgcatttat catcaaaaac gtttaacgca
     4981 ggctattata gtcatcaacc atttcgttgt actcgcgtaa cgctcgtgga aatgagtctt
     5041 cgaccatcca gcttgtgttt gcatcctgaa gatgctcttt atcccagtca gagtacggga
     5101 ctttatctct gacgcggcga acatattttt tagtctcgag ttgatactgg tctgccgaat
     5161 taatgaacga gaaattcatg ccgcctttat ccgcttcttt ggcctgcgcc accagcgttt
     5221 ccagttcaga cactttcttc attgccgcat caacatcaaa cttctcttgc gatatcaggt
     5281 tattaatttg tttcgctgaa atcatgactg ccagagaata gtagtggaat gttttccctt
     5341 ctctttcttc aatatttttc agttccgcaa gctggcgctt atcattaatt tcctgaatcg
     5401 ccgcatgata agattctgct acaggttcaa aggcttccag attttttaag aatgtctggt
     5461 gcagcgtttt ccctttcgca aacgcatcgt ctttataatt ttcctgggtg taatatttat
     5521 ccatttcgtt aatggtatta cccaacgcca cggcagcatc gatataactc accgccacgc
     5581 catcaattgg ttgcagcgca ggcgttaacg ccacagcact ttttacgcct ttttcacact
     5641 cagcgaggtt ggattcacta atgccgtaga tgccataaac agtactctct ttaccggtag
     5701 gcccctgttt aaaatctttc agccagtcag cataacgcgc cagactgcgc tgtaccggga
     5761 tctgtaactt gttgtagcac ttgatataga catccatttt ttcgtcgaaa accgcttgtt
     5821 catcgaccag cggttgcgca ggtgtttcag gtttagcatc tgcttttgca acgggagctt
     5881 cagtaggttt cgcttcagga gctggtgcgg caggttgact attggcaggc aggggcgttt
     5941 ctgtttcggc ttttttatca tcacaacccg tcagaccgag ggccatgatg gcgactataa
     6001 tagcggaaga taataaattc ctt
//
LOCUS       DAZKFZ010000248         6012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000248 DAZKFZ010000000
VERSION     DAZKFZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            100..234
                     /locus_tag="ACHOQW_004297"
     CDS             100..234
                     /locus_tag="ACHOQW_004297"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575253.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            836..1657
                     /locus_tag="ACHOQW_004298"
     CDS             836..1657
                     /locus_tag="ACHOQW_004298"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9575254.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1657..2763
                     /locus_tag="ACHOQW_004299"
     CDS             1657..2763
                     /locus_tag="ACHOQW_004299"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9575255.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2853..4574
                     /gene="cptA"
                     /locus_tag="ACHOQW_004300"
     CDS             2853..4574
                     /gene="cptA"
                     /locus_tag="ACHOQW_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9575256.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4648..5646
                     /gene="lpxM"
                     /locus_tag="ACHOQW_004301"
                     /gene_synonym="msbB"
     CDS             4648..5646
                     /gene="lpxM"
                     /locus_tag="ACHOQW_004301"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9575257.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5699..5887
                     /locus_tag="ACHOQW_004302"
                     /pseudo
     CDS             5699..5887
                     /locus_tag="ACHOQW_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1541 a         1315 c         1518 g         1638 t
ORIGIN      
        1 taaactaata tcgactttca ataaataagg acaggtaatg gacaacaaaa atatctgaac
       61 ccgggtgaga ttcataagtt gcacagtgct gtcttcaaag tgccacaccc ggaacgtaat
      121 cactgcctgc tccttatggg gtatcttcat ggtgtccaag catcagagtt attaggtata
      181 aaactgtctg atattgattt gcaggccggt aacctgaata tccggcggct ctaaacggtt
      241 tgtcaactca gcccctcggc tgaaacagga ggtacagtcc attcgtcgct ggctgaaact
      301 tcgggatgaa tgggtagaag agggatatga atggttattt aacctctatg gcaggtctgc
      361 tacaggtatt actgttgttc tggggggcac tggtgtggtt aatatctacg atgcaagaga
      421 ttctcacgat ggcccggtcc atcagtgcat gcggcgcgat attgtgcgag tatttttcag
      481 ggggagtggt atccggatgc agaaaattcc gctactcgac tggttttatg caataaatca
      541 ggatgataat cttctgattt tttactgagg cggatatctc tcgctgtgtt tactgaaatt
      601 gcggtattct gttttttctg aaaaacagaa aatctgatta aaaagagcat atgcgttaag
      661 ggaaatttaa gttctggttg attaactgat aaaaatatta aaaaatccct gttgtcggta
      721 atctccggac cattccggga ttacacaata atatcaccat gtgtgatatc tatggatcct
      781 cactgttgtt tacgcgagga tattttcaga atgcttaaca gacgattatt tttttatgtt
      841 gaatgcaagg catcttcccg tactgatgta tcatcatgtc agtcgttgtc cggggcttgt
      901 gacactctca cctgaaacat tttgtgaaca gatgaagtgg ctcgcagata ataactggcg
      961 gacggtaaca tctgctgagc ttgaatattt ttatcagggg ggaacgcttc cacgaaagag
     1021 tgtcatgctc acattcgatg atggttatct tgataactgg ctcagagcat ggccggtcct
     1081 gaaggaatat aaccttcatg cacatatttt tctcattacg gggctgattg gtaagggaaa
     1141 cgtacgtagc aggcaggagc aggaatattc tcaccgcgac tgtgaacggt tgattgccga
     1201 taaccgttca gatgaggtca tgcttcgctg gtctgaagtc cgggaaatgc gtgattccgg
     1261 actggttgaa tttcatgtgc acacccattc acataaacgc tgggacaggt tgtctgtatc
     1321 acgtgctgaa cagtgccggc tcatgaaaga agatattctg gtgggaaaac aatgtctgac
     1381 ggaaaagctg gggttctgta gttcccatct gtgctggcct gaaggatatt ataatcggga
     1441 ttatattaat ctcgccggta agcttggttt ctcttattta tatacaaccg aaagaagaat
     1501 gaactgtcct gaaaatggtt cattacgcat agggcgtatc agcaccaaag agcgggaaca
     1561 ttccggctgg ctgaaacgac gcctttttta ttatacaacg ccactttttt cttcagtact
     1621 ggctcttcat aaggggccaa gattaccaga taattaatga tgaaaatttt attcactgaa
     1681 tcttcgtcag atattggggg ccaggagctg caggcactgg cccagatgac tgctcttcaa
     1741 aagcagggac attccgtttt acttgcctgc agagaaaaaa gtaaaattgc tccggaagcc
     1801 agaaaaagag ggcatgatgt cacttttatt cctttcagga acagccttca ccttccttcc
     1861 atcctcagac ttcgacggat tatcggggaa tttaaacctg atctggtcat ctgccatagt
     1921 ggccatgaca gtaatatagc aggactgagc cgtctgatat gctgtcaccg cttcagtatt
     1981 gtcagacaaa aaacctacat cacccgtaag accagaacgt tttccctgaa ttatctgtgt
     2041 gatttcattg ttgtgcctag ctcagccatg atggcgcatc tgatggcgga aggtgtcagg
     2101 acaccggtaa cggttattcc accgggtttc gactggcccg cattgcataa tgaagccatg
     2161 cgaccgttac ccctgcatat ccacgcctgg gctgcatcag cagacaatgt tccgcttatc
     2221 gttcaggtgg ggatgctacg tcccgaaaaa ggacatgaat ttatgctgcg tgtattgtat
     2281 cagctgaaga tggaagggaa atcatttcgc tggctcgtgg tgggcgccgg gcgtgaagaa
     2341 tacgaagcac gtctgcgtca gcagacagaa cacctgggaa tgagcggaga tgtgttaatg
     2401 gcaggtgccc ttttcccagc gttaccggtc taccggattg ccagtgtggt ggtcatgcct
     2461 tcggaaaatg aagcgtttgg tatggtactg gctgaagcgt cagtctccgg cgtgcccgtt
     2521 atcgccagtg agacgggcgg gatccctgat gtcattcaga agaatgtgac agggacgctg
     2581 ttacccgtgg gggatgtgtc tgcatggacc ggcgcactgc gcgatttttt atcccgaccg
     2641 gaacgcttcc ggatgatggc agccagcgcc agagaagata ttgagtaccg gtttgatatt
     2701 aacagaacgg cacagatcat tgtgtctctg gctagtcagg ctaaggggaa atgcaacaga
     2761 taacggcgga tcctcttttc agcccgaatt ttgttcagcg gttttccggt aacggagtaa
     2821 tttatcacat tgtttaaata cggggacggt caatgcattt aaataccgga cagaacaggc
     2881 ctactttcag ctggagcgcg ctgggatggg caatttttta ttttggcttt ttttccactc
     2941 tcctgcaggt catcatcttc agcagtgggt acagcggaac gaatggaata cgggactcac
     3001 tgttattcag ttgtctgtgg ttgatcccgg tgtttctcta tcctgatcgg ataaaaataa
     3061 ttgcagctgt tgtcggtttc attctctggg gcacgtcgct ggcagcactt tgttattatt
     3121 ttctctatgg tcatgaattc tctcaaagtg ttcttttcgt tatgtttgaa acgaatgcca
     3181 gagaggctgg tgaatatttc agccagtatt tcagccttaa actgttgctt atctcgctgg
     3241 tatatactgc ggtgtccgtt tttctgtgga cacgtctgcg tcctgtatat attcctttgc
     3301 catggcggag aattgtctct ttcctgctgc tttatgctct gcttctgcat ccggttgttc
     3361 tgaaatcgtt aatcagacag gagccgctga atgatactct aggcaaactg gcatcccgaa
     3421 tggagcctgc tgccccctgg cagtttgtat ccagttatta tcagtaccat cagcaactga
     3481 atgcactgac aaccttcctg aatgaaaata gcgcactgcc accactgggt aatctcaggg
     3541 atgaatcagg ggagagacca cgcacactgg tcctggtgat tggtgagtcg acacagcgcg
     3601 aacgcatgag cttatacggg tatctacgtg aaacgacgcc ggagctggat gcactgcgta
     3661 aaaccgatcc gggtcttact gtgtttaata atgtggtggc atcgcgtccg tacaccattg
     3721 aagcattgca acaggccctt actttcgcca acgaaaagaa tcctgatctg tatctgacgc
     3781 agccgtcgct gatgaacatg atgaagcagg caggctataa aacattctgg attaccaacc
     3841 agcagacaat cacagcccgt aacaccatgc tcactgtatt ttcgcgccag acggacaggc
     3901 agtactacat gaatcagcag cgaacacaaa gtgcgcgtga atatgacact aacgtgctga
     3961 agccgttccg ggaagtgctg aatgaccctg caccaaagaa actgatcatc gtgcatctgc
     4021 tgggtacgca cattaagtat aaataccgtt atccggaagg tcaggggcgg tttgatggca
     4081 ttacagggca tattcccact ggattaaatg cgaaagagct ggaagtgtat aacgattacg
     4141 ataatgccaa tctgtttaac gatcatgtgg tggccagtct gataaaggac ttcagagcaa
     4201 ctgcgccgga cggctttctg ctttattttt cagaccatgg tgaagaagta tatgatactc
     4261 cgccatataa aacgcaggga cggaatgaag ataaccccac acgtcccatg tacactgttc
     4321 cgttcctgct gtggacctcg gaaaagtggc atgctgcaca tccgcgagat ttttcgcagt
     4381 atgttgaccg caaatacagt ctggctgaac tgatccacac ctggtcagat ttggcgggac
     4441 tgacatacga tggttacgat ccgacccgtt ctctggtgaa tccgcaattc agggaaacca
     4501 cccgctggat tggaaatccg tataaaaaga atgggctcac tgatttcgac actcttccgt
     4561 atggtgagcc atagtatttt cttatcttcg gttccgttct gaaggcagga ggatgtgttt
     4621 tcctctgctg tccggtggcc tccagggatg gtaccacctt ctgctgtact ctgttatcat
     4681 aatgagataa gcaggcagat accggtaaat atgaaaaata tcagaactga gtttattccc
     4741 cgctttaacc ttacattatg cttcccccgt tactggatga cgtggacagg tatcggtatt
     4801 atctgtgtgt ttgcaatggt cccccccgca ctgagagatc cattgttggg aaaattgggc
     4861 atgctggttg ggcgtctggg gaaaagcgca cgtcaacggg ccttgattaa tttatcgctt
     4921 tgttttccgg agtacagtga taaagaaaag gagaatatcg ttgatgccat gtttgcaaca
     4981 gcctccatgg ccgttgtgct tatggctgaa ttagcactgt ccgggcctga taaaatttcg
     5041 catcgtatac gctggaatgg tctggagatt gtggagaaga tggcacagaa caatgaaaag
     5101 gtcatttttc ttgtgccaca tgcctgggga gtggatattc ccgcaatgct gatggctgcg
     5161 tcgggcagaa aaatggcagc gatgtttcat aaccagagaa atccggtggt ggattatgtc
     5221 tggaacagtg ttcgtcgtcg tttcggagga aaactgcacg cacgtaatga cggtattgcc
     5281 tcctttgtcc gctcagtccg gcagggatac tggggatatt atcttcctga tcaggatcat
     5341 ggcccggaat tcagtgagtt tgcagatttt tttgcgacgt ataaagcaac tttaccggtt
     5401 atcggacggc tcagcaggat cagtggtgca cggataatac cgcttttccc tgtttatgac
     5461 ggaaagaccc atcatctgac tattcatgtc agcccgccac ttgctatacg acagaagagt
     5521 gatgctcata ttgcgcgaca gataaacgaa gtggttgaaa atttcgttcg tccacatcct
     5581 gagcagtata cgtggatcct caagttgctg aaaacgcgta aagaagggga agaggatcct
     5641 tactgaggca gctttttcag gataatgagc ttacccagca atagtggaca cacgctaagt
     5701 gagtaaactc tcagtcagag gtggctcata tgacaaaaaa acagtaccaa ccagtaaaaa
     5761 accccgtaaa tagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg ctgaacgcat
     5821 cggtgttgct gtcgcagctc gtgtgctcag tctgtatgaa tcacagcttg taagcggctc
     5881 gtcagaactg tattgatatt tactgagcgg tcagttcaac gtaagcgagt gttatgcgcc
     5941 atggaagctt acccaggaat atgttatcag gcctcagcat ggcgaagtgt tgcaacgctg
     6001 gaagcagaat ga
//
LOCUS       DAZKFZ010000249         5994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000249 DAZKFZ010000000
VERSION     DAZKFZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            165..683
                     /locus_tag="ACHOQW_004303"
     CDS             165..683
                     /locus_tag="ACHOQW_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9575258.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            893..3034
                     /locus_tag="ACHOQW_004304"
     CDS             893..3034
                     /locus_tag="ACHOQW_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9575259.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3110..>5994
                     /locus_tag="ACHOQW_004305"
     CDS             3110..>5994
                     /locus_tag="ACHOQW_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9575260.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDGSRSEI
                     QYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
BASE COUNT         1370 a         1597 c         1930 g         1097 t
ORIGIN      
        1 attttaaaaa tagtttttac gacaaccgta aattctagct cggtttgtat gtaaaaaata
       61 ctgaaaaatg atgcggttcc gtgaagtgag ctgcatctgc ctgtctggga ttaacggcag
      121 gtcattgtaa gtagtaatta accacgttaa caaggagttt acaaatgcca acaccgtgtt
      181 atatctccat cactggtcag acccagggaa atattactgc aggtgcattt accgctgatt
      241 ctgtcggcaa catctatgtg caggggcacg aagacgagat gctggtgcag gaattcctgc
      301 ataacgttac tgtcccgacc gatcctcagt ccggtcagcc tgcgggtcag cgtgctcaca
      361 agcctttcat ttttaccgtg gcgctgaaca aagcggttcc gctgatgtac aacgcactgg
      421 cctccggcga aatgctgcca accactgaac ttcactggtg gcgtacctct gtggaaggca
      481 aacaggagca ttatttcact acccgcctga ctgattcgac gattgtcgat atgaaactgc
      541 atatgccgca ttgccaggac ccggcgaaac gcgagttcac ccagttgctc gaagtttcac
      601 tggcctaccg caaaattgaa tgggaacacg tcaaatctgg cacctccggt gccgacgact
      661 ggcgcgcacc gctggaagca taagttaagc caacagcatc cggctatcag gccggatgct
      721 gtttttgtat tacgccgtag aaagaaaggt tgtcagtaag gggttacgtt cacgggagat
      781 ggcggctgcc ggaaacttcg gcggtagcgt gcctgaacgc aagcgtagca ctgtactgtc
      841 acagccgctt taacagcgaa ctgtctgttt ttccttaacc ccggaggttg ctatgtcaac
      901 cggattacgt ttcacgctgg aagtggacgg cctgccaccg gatgcttttg cggtggtctc
      961 ctttcatctg aaccagtcac tctcttcgct tttttccctc gatctctctc tggtcagcca
     1021 gcagtttctc tcccttgaat tccagcagat cctcgacaaa atggcctacc tgacgatatg
     1081 gcagggcgat gacgtacagc gccgggtgaa aggtgtggtg acctggtttg aactggggga
     1141 gaacgacaaa aaccagaagc tgtacagcat gaaggtatgc ccgccgctgt ggcgcacagg
     1201 gctgcgccag aacttccgta tcttccagaa tgaggacatc gcaagcatcc tcggcacgat
     1261 attgcaggaa aacggggtga ccgagtggag cccgctgttc agcgagccac atccttcccg
     1321 tgagttttgt gtccagtacg gtgagactga ttacgatttc ctgtgccgga tggcggcgga
     1381 ggaaggcatc ttcttttatg aggagcacgc gcaaaaaagt accgaccaga gcctggtcct
     1441 gtgcgatacc gtgctttatc tgccggagtc ctttgagatc ccctggaatc cgaacacccg
     1501 taccgaggta agcaccctct gcatcagcca gtttcgctac agcgcacaaa tccgcccttc
     1561 ttccgtggtg accaaagact acacctttaa acgccccggc tgggccggac gttttgacca
     1621 ggaaggccag caccaggatt accagcgcac acagtatgaa gtgtatgact atcccggacg
     1681 tttcaagggc gcccacgggc agaactttgc ccgctggcag atggacggct ggcgaaacaa
     1741 cgcagaagtg gcgcgcggaa caagccgttc gccggagata tggccgggac ggcgaattgt
     1801 gctgacgggg catccgcagg cgaacctgaa ccgggaatgg caggtggtgg caagtgaact
     1861 gcacggtgag cagccgcagg cggtgccggg acgcaggggt tcaggcacca cgctggataa
     1921 ccattttgcg gtaataccgg cagacagaac atggcgacca cagccgttgc tgaaaccgct
     1981 ggtggacggc ccgcagagcg ccgtcgtgac gggaccggca ggcgaggaaa tcttctgtga
     2041 tgaacatggc cgcgtgcggg tgaaatttaa ctgggaccgc tataacccgt caaaccagga
     2101 cagttcatgc tggatccgtg tggcacaggc gtgggcaggc accgggtttg gtaacctggc
     2161 gataccgcgt gtgggtcagg aggtcattgt ggacttcctc aacggcgatc cggaccagcc
     2221 gatcattatg gggcgtacct accaccagga aaaccgcaca cccggtagcc tgccggggac
     2281 gaagacgcag atgaccattc gttcgaaaac ctataagggc agcgggttta atgaactgaa
     2341 gtttgacgat gcgacaggga aagaacaggt ctacatccac gcgcagaaga acatgaacac
     2401 cgaggtgctg aataaccgca ccacggatgt gataaacaac catgccgaaa aaataggtaa
     2461 caaccaggcg atcaccgtta ccaataacca gatcctgaac attggcgtta atcagataca
     2521 gacggttggt gtcaaccagg tggaaacggt aggcagtaac cagattatca aagtgggatc
     2581 aaaccaggtt gaaaaggtgg ggatcattcg tgcgctgacg gtgggtgtgg cgtatcagac
     2641 gacggtaggc ggcattatga atacctcggt ggcgttgttg cagtcctcac aggtagggct
     2701 gcataaatca ctgatggtgg gaatgggcta cagcgtcaat gtagggaata acgtcacctt
     2761 ctcggtgggc aagacgatga aggaaaacac cggacaaaca gcagtttatt ctgccggtga
     2821 gcatcttgaa ctctgctgtg gtaaggcaag gctggtgctg acgaaggacg gaagcatatt
     2881 tcttaatggt acgcacattc atctggaagg ggagtcggat gtgaacggtg atgcgccggt
     2941 gattaactgg aactgtggtg ccacacaacc tgtaccggat gcgcctgtgc cgaaagattt
     3001 accccccgga atgccggata tgcggcaatt ttgagtcagg tgcaaaaaat caaaacggaa
     3061 ctggtgcagt gaccagataa accatattaa aagcagaagg aattgattca tgggaggaaa
     3121 accggcggcg cggcagggtg acatgacccg caagggactg gatattgtgc agggttcagc
     3181 aggggtgctg ataggtgcgc cgacgggcgt ggcctgctcg gtgtgtccgg gagggattac
     3241 ctatgctaac ccggtgaacc cggtgctggg tgcgaaggtg ctgccgggcg agacggacct
     3301 tgcgctgccc ggcccgctgc cgtttattct ttcccgcgcc tacagcagct accggaccag
     3361 aacacccgcg ccggtggggg tgtttggtcc cggctggaaa gcgcccttcg atatccgctt
     3421 acagatacgc gatgaaggcc tgatactcaa cgacaacggc ggcaggagca ttcactttga
     3481 gccgctgttt cccggcgaga taagctacag ccgcagcgag tcgttctggc tggcgcgggg
     3541 cggggtggcg gagcagcaca gttcgcagcc gctaagcgcg ctctggcagg tgctgccgga
     3601 agatgttcgc ctgagtccgc atatgtacct ggcgacaaac agccttcagg ggccgtggtg
     3661 gatactcaac tggccggagc gggtgccggg ggcggacgag gtgctgccgc cggagccgcc
     3721 cgcataccgg gtgctgacgg gcgtggtgga tggcttcggg cggacgctgg cctttcaccg
     3781 ggcggcggag ggtgatgtgg cgggcgcggt gacgggggtg acggacggcg cgggccgccg
     3841 ttttcacctg gtgctgacca cacaggcaca gcgggcggaa gtattccgta aacagcgcgc
     3901 cacgtcttta tcttcccctg ccggtccccg ttctgcttcc tcttctctgg ttttccccga
     3961 cacgctgccc gccggtacag aatacggtgc cgataacggt atccggctgg aggcggtatg
     4021 gctgacacac gacccggcat acccggatga actgcccgcc gcgccgctgg cgcgctacac
     4081 gtacacggcc agcggagaac tgcgggcggt gtatgaccgc agcgggacgc aggtgcgcgg
     4141 gtttgcttat gatgcggagc acgccgggcg gatggtggcg caccattatg cgggtaggcc
     4201 ggagagccgc taccggtatg atgataccgg ccgggtgacg gagctggtca acccggaggg
     4261 gctggactac cgctttgagt acgggcagga ccgtgtgacc atcacggaca gcctgaaccg
     4321 gcgggaggtg ctgtacacgg aaggcgaggg tggcctgaaa cgtgttgtga agaaggaaca
     4381 tgcggacggg agcatcaccc gcagcgagta tgatgaggcg gggaggctga aggcacagac
     4441 ggatgcggcg ggacggcgga cggagtacag cctgcatatg gcgtcgggtg cggtgacagc
     4501 ggtgacgggg ccggacggca ggacggtgcg gtatggctat aacagccagc ggcaggtgac
     4561 gtcagtgacg tacccggacg ggctgcgcag cagccgggag tatgatgaga agggaaggct
     4621 ggcggcggag acctcgcgca gcggagagac gacgcggtac agctatgatg acccggcgag
     4681 tgagctgccg acagggatac aggacgcgac gggcagtaca aaacagatgg catggagccg
     4741 ttacggtcag ctgctgacct ttacggactg ctcggggtac acgacgcggt atgagtatga
     4801 ccggtacggt caacaaatcg ccgttcaccg ggaagaaggc atcagcactt acagcagtta
     4861 taacccgcgt ggccaactgg tcagtcagaa ggatgcgcag gggcgtgaaa cccgttatga
     4921 gtacagcgcc gcaggcgacc tcaccgctat cgttgccccg gacggcagcc gcagtgagat
     4981 acagtatgat gcgtggggaa aggccgtcag caccacgcag ggcggtctga cgcgcagcat
     5041 ggggtatgac gctgccgggc gcatcaccgt gctgaccaac gagaacggca gccagtccac
     5101 gttccggtat gacccggtgg acaggctgac tgaacagcgc ggttttgacg gccggacgca
     5161 acgttaccac tatgacctga ccggaaaact cacgcagagt gaagacgagg ggcttatcac
     5221 cctctggcac tacgatgcgt cggaccgcat cacgcaccgg acggtgaacg gcgacccggc
     5281 agagcagtgg cagtatgatg agcacgggtg gctaaccacc ctcagccata ccagtgaagg
     5341 ccaccgggtg tcggtccact acggctatga cgataaaggc cgcctgacgg gtgaacggca
     5401 gacggtggag aacccggaga cgggggagat gctgtgggag catgagacgg ggcacgcgta
     5461 cagcgaacag gggctggcga cccgtcagga gccggacggt ctgccgccgg tagagtggct
     5521 gacgtatggc agcggttatc ttgcggggat gaagctgggc ggaacgccac tggtcgagta
     5581 catgcgggac cggctgcacc gtgagacggc ccgcagcttc ggcggggagg catatgaact
     5641 tgccaccgcc tggaatacca gcggccagct ccggagcagg cacctgaacc ttccgcagct
     5701 tgaccgtgac tacgactgga acgacaacgg acagctaatc cgcatcagcg gcccgcagga
     5761 gagccgggag taccgttaca gtgacacggg aaggctgacg ggcgtccaca ccacggcagc
     5821 gaacctggat atcgatatcc cgtatgcaac ggacccggca ggaaaccggc tgccggaccc
     5881 ggaactgcat ccggacagca cgctcacggc gtggccggat aaccgcatcg cggaagatgc
     5941 gcactatatc tatcgctacg atgaatacgg caggctggcg gagaagacgg accg
//
LOCUS       DAZKFZ010000250         5994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000250 DAZKFZ010000000
VERSION     DAZKFZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..463
                     /locus_tag="ACHOQW_004306"
     CDS             <1..463
                     /locus_tag="ACHOQW_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575261.1"
                     /translation="ADYEFSSSASWVDVDATGKVTFKNVGSKWERITATPKTGGPSYI
                     YEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSRGIGSLYSEWGDMG
                     HYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(725..1777)
                     /locus_tag="ACHOQW_004307"
     CDS             complement(725..1777)
                     /locus_tag="ACHOQW_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP9575262.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            2091..3407
                     /gene="shiA"
                     /locus_tag="ACHOQW_004308"
     CDS             2091..3407
                     /gene="shiA"
                     /locus_tag="ACHOQW_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP9575263.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            3509..4963
                     /gene="amn"
                     /locus_tag="ACHOQW_004309"
     CDS             3509..4963
                     /gene="amn"
                     /locus_tag="ACHOQW_004309"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP9575264.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            5306..>5994
                     /locus_tag="ACHOQW_004310"
     CDS             5306..>5994
                     /locus_tag="ACHOQW_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9575265.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT         1576 a         1332 c         1338 g         1748 t
ORIGIN      
        1 ggctgattat gagttttcaa gctctgcctc ctgggttgat gttgatgcta ccggtaaagt
       61 gacatttaaa aatgtcggca gcaaatggga gaggattacg gcgacgccaa aaacaggcgg
      121 ccctagctat atatacgaaa tccgagtgaa gagttggtgg gtgaacgccg gcgatgcttt
      181 catgatatac agccttgctg aaaatttttg cagtagcaat ggctacacac ttccccttgg
      241 agaccattta aaccatagtc gttcccgagg catcgggtca ctgtacagtg aatggggaga
      301 tatggggcat tacacgactg aagctggttt tcattcaaat atgtattggt catcgagtcc
      361 cgcaaactca aacgaacaat acgtagtttc cctggcaaca ggtgatcaaa gcgtatttga
      421 aaagcttggg tttgcttatg cgacatgtta taaaaacctc tgattttccg ttataatttc
      481 ttaaagagtg ctccattcgg ggcactcttt tttacattcc ctctatagag cttgcccttc
      541 tctcacagaa aatgaataaa taaaaatgcg ataccgcaag aatcgcgttc gatgtgacta
      601 tgatcgtatc cggtgagtac agcttttatc caaaggctgg ttttctgtga tgattgacca
      661 gcctcagagt attgatgaca ccaccgttca taacagatcg gcattactat gcataagtac
      721 tgtctcatat tctcgccaaa acacttttta atgcctttgc gaattcatcc agacttatat
      781 tgtcaatttt ttgcccaacg acgactgact ccgaaacatc ggccattgcg ggccatcgcg
      841 ttttgtaatc agcataaatt gcaacaacgg gggtattata ggcacttgcc atatgtaata
      901 ttgaggtgtc aggagttatt gcgatataag catcttttat gattgctgct gaacgtttaa
      961 tcgagggtga taaggataaa cggtagacat tattataaca atccactaat accctggctt
     1021 tatcttcccc ttttggtcca tgaacgatca ctattggtat atctgtttca ctttgaattt
     1081 tttctattag attttctgca atcgatagtg aaaatgtacg atcctgcgag ctaccttcta
     1141 aattaagcgc aatgtaagat cctaacgatc gcatttcctc gcgcacctca tcgagtacat
     1201 cctcacttag tggcaattca tatattggcc tgactgctgg aaaacccgcc tcacgcatca
     1261 taaatgccca tgtcatgggt acgggagccc ggagtttctg atccatccgg gacgcgttct
     1321 tacacacagg ggaatagcac ttcatggtta aaccaacaac ctgaaaattc gtttttgctt
     1381 tcaactgact gataaataac atcgttttca ggttcttttt acgcatcgcc tctatacaaa
     1441 gatccggcgt accgtattgc tgtgttatgt tcttcgctaa atcttttatt tcttttaatg
     1501 ttgcgtgatc ctgcatagtc attgtgacta atgttaattt agtctgttca agtttgagcg
     1561 cattaaagac ttctaaatta attgtcgacg taacaattaa tagatgctta attttatgca
     1621 attcaagcgc ccgaataaca ggaaagatgg ccatagcatc gccaatctga tcgggaatat
     1681 ggatgacaac aaagtctgtt ttttcaatat tgaaattata agctttataa tcgtagtaac
     1741 taaatgcaat acgtctcaac aatgatacta aaaacataac taacctcgcc tccctactgg
     1801 ttataatgca atgcagtcta tcagaatcat cagggtgcaa ttttgtgcat atgcggactt
     1861 ttatgtttca tatctcgaac ctgtggggcc tctgcttaat ccttaaacaa caccagcaac
     1921 tcctgcgctt tcatcttcca gcgaattttt catgttgccg ttaatcagcc ataaaatcat
     1981 ttgcagatgc gctctgtcga ggtagtctca taaggttcgt ttatagatcg acgcaatgtg
     2041 agttaccttt tccatactaa ttataaaaag acagtacaaa caggatcatt atggactcca
     2101 cgctcatctc cactcgtccc gatgaaagga cggtttcgtt aagtcgcgcc cgacgagctg
     2161 cgttaggcag tttcgctggt gccgtcgtcg actggtatga ttttttactc tatggcatta
     2221 ccgccgcact ggtgtttaat cgcgagtttt tcccgcaagt aagcccggct atgggaacgc
     2281 tcgccgcatt tgccaccttt ggcgtcggat tccttttccg tccgctcggc ggtgtcattt
     2341 tcggtcactt tggcgaccga ctgggacgta agcgcatgtt aatgctgacc gtctggatga
     2401 tgggcatcgc gacagccttg attggtattc ttccttcatt ctcgaccatt gggtggtggg
     2461 cacctatttt actggtgacg ctgcgtgcca ttcagggatt tgccgtcggc ggcgaatggg
     2521 gaggcgcggc gttgctttcc gttgaaagtg caccgaaaaa taaaaaagcc ttttacagta
     2581 gcggtgtaca agttggctac ggtgtaggtt tactgctttc aaccggactg gtttcattga
     2641 tcagtatgat gacgactgac gaacagtttt taagctgggg ctggcgcatt cctttcctgt
     2701 ttagcatcgt actggtactg ggagcattgt gggtgcgcaa tggcatggaa gagtccgcgg
     2761 aatttgaaca acagcaatat aatcaagcgg ccgcgaaaaa acgcatcccg gttatcgaag
     2821 cgctgttacg acatcccggt gctttcctga agattattgc gctacgactg tgcgagttgc
     2881 tgacaatgta catcgttacc gcctttgcac ttaattattc aactcagaat atggggttac
     2941 cgcgcgaact tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc
     3001 cctgttttgc ctggcttgcc gatcgttttg gtcgccgcag ggtttatatc acaggcgcgt
     3061 tgatcggaac gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct
     3121 ggatagtttt cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc
     3181 aacaaccgat gtttaccgaa atgtttggtg ccagttatcg ctatagtggt gctggagtcg
     3241 gttatcaggt tgccagtgtg gttggcggtg gatttacacc ttttattgcc gctgcgctca
     3301 tcacttactt tgccgggaac tggcatagcg tcgccattta tttgctggct ggatgtctga
     3361 tttccgcaat gaccgctttg ttgatgaaag acaatcaacg cgcttgatag tctggcgaag
     3421 atcatccgat cttcgcctta cacttttgtt tcacattcct gtgacatact atcggatgtg
     3481 cggtaattgt atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc
     3541 tcaggcactg gataaactcg acgcgctgta tgagcaatct gtagtcgcat tacgcaacgc
     3601 cattggcaac tatattacaa gtggcgaatt acctgatgaa aacgcccgca aacaaggtct
     3661 ttttgtctat ccatctctga ccgtaacctg ggacggtagc acaacaaatc cccccaaaac
     3721 gcgcgcattt ggtcgtttta ctcacgcagg cagctacacc accacgatta ctcgccctac
     3781 tctctttcgt tcgtatctta atgaacaact tacgttgctg tatcaggatt atggcgcgca
     3841 tatctcggtg caaccctcac agcatgaaat cccttatcct tatgtcatcg atggctctga
     3901 attaacactt gatcgctcaa tgagcgctgg gttaactcgc tatttcccga caacagaact
     3961 ggcgcaaatt ggcgatgaaa ctgcagacgg catttatcat ccaactgaat tctccccgct
     4021 atcgcatttt gatgcgcgcc gcgtcgattt ttccctcgcg cggttgcgcc attataccgg
     4081 tacgccagtt gaacattttc agccgttcgt cttgtttacc aactacacac gttatgtgga
     4141 tgaattcgtt cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct
     4201 ttcttgtgct ggcgggaact ggatcaccgc cgaaaccgaa gcgccagaag aagccatttc
     4261 cgaccttgca tggaaaaaac accagatgcc agcatggcat ttaattaccg ccgatggtca
     4321 gggtattact ctggtgaata ttggcgtggg accgtcaaat gctaaaacca tctgcgatca
     4381 tctggcagtg ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga
     4441 aagtcaggcc attggcgatt atgtacttgc acacgcttat ttacgcgatg accacgttct
     4501 tgatgcggtt ctgccacccg atattcctat tccgagcatt gctgaagtgc aacgtgcgct
     4561 ttatgacgcc accaagctgg tgagcggcag gcccggtgag gaagtcaaac agcggctacg
     4621 tactggtact gtggtaacca cagatgacag gaactgggaa ttacgttact cagcttctgc
     4681 acttcgtttt aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc
     4741 gcaaggatat cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt
     4801 gcatggcgag attaaacttc ccggtcaggc taaccgtttt tatgaaggcg ctatttccga
     4861 acacctacaa attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca
     4921 ttcacgtaaa ttacgaacct ttaatgagcc gccgttccga taataagttc tgaaccttct
     4981 tatcaaaaat agatgtgaag gattaagtaa gacatctggc aaatagcccg caataaacgc
     5041 gggctgtttg cttccttggg cggatacgag ttttattatc gtcttaatga tttccacata
     5101 ttaaaagcaa gtatgctttc aaaacacaat tataaaaaat cccgccaaca atataagttt
     5161 ttataaaatt aaatataaga ttatggcttt agaatatttt tatttctaat agacgagatt
     5221 tttcctgtta tgatataata tgccgaatta acacatgtta acgatttacc agtaatgtaa
     5281 ataaattttc gaggagatca ttcctgtggg acgtaaatgg gccaatattg ttgctaaaaa
     5341 aacggctaaa gacggtgcaa cgtctaaaat ttatgcaaaa ttcggtgtag aaatctatgc
     5401 tgccgctaaa caaggtgaac ccgatccaga attaaacaca tctttaaaat tcgttattga
     5461 acgtgcaaag caggcacaag ttccaaagca cgttattgat aaagcaattg ataaagccaa
     5521 aggcggcgga gatgaaacat tcgtgcaggg ccgttatgaa ggctttggtc ctaatggctc
     5581 aatgattatc gccgagacat tgacttcaaa tgttaaccgt acgattgcta acgttcgcac
     5641 aattttcaat aaaaaaggcg gcaatatcgg agcggcaggt tctgtcagct atatgtttga
     5701 caatactggt gtgattgtat ttaaaggatc agaccctgac catatttttg aaattttact
     5761 tgaagctgaa gttgatgttc gtgatgtgac tgaagaagaa ggtaacattg ttatttatac
     5821 cgaacctact gaccttcata aaggaatcgc ggctctaaaa gcagctggaa tcagtgagtt
     5881 ctcaacaaca gaattagaaa tgattgctca atctgaagtt gagctttcac cagaagattt
     5941 agaaatcttt gaagggcttg ttgatgccct tgaagatgac gacgatgtac aaaa
//
LOCUS       DAZKFZ010000251         5910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000251 DAZKFZ010000000
VERSION     DAZKFZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            28..1141
                     /gene="ompN"
                     /locus_tag="ACHOQW_004311"
                     /pseudo
     CDS             28..1141
                     /gene="ompN"
                     /locus_tag="ACHOQW_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            1282..1716
                     /gene="uspF"
                     /locus_tag="ACHOQW_004312"
     CDS             1282..1716
                     /gene="uspF"
                     /locus_tag="ACHOQW_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP9575266.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1892..2827
                     /gene="ttcA"
                     /locus_tag="ACHOQW_004313"
     CDS             1892..2827
                     /gene="ttcA"
                     /locus_tag="ACHOQW_004313"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP9575267.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2956..4329)
                     /gene="dbpA"
                     /locus_tag="ACHOQW_004314"
     CDS             complement(2956..4329)
                     /gene="dbpA"
                     /locus_tag="ACHOQW_004314"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP9575268.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4362..4532)
                     /gene="ynaL"
                     /locus_tag="ACHOQW_004315"
     CDS             complement(4362..4532)
                     /gene="ynaL"
                     /locus_tag="ACHOQW_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP9575269.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            complement(4807..5790)
                     /gene="zntB"
                     /locus_tag="ACHOQW_004316"
     CDS             complement(4807..5790)
                     /gene="zntB"
                     /locus_tag="ACHOQW_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP9575270.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5804..5851)
                     /gene="ydaN"
                     /locus_tag="ACHOQW_004317"
     CDS             complement(5804..5851)
                     /gene="ydaN"
                     /locus_tag="ACHOQW_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP9575271.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         1567 a         1491 c         1395 g         1457 t
ORIGIN      
        1 aaataaaata actaaaggat ttattcaatg aaaagcaaag tactggcact tttaattcct
       61 gccctgctcg gcgcaggtgc tgcacatgca gccgaagttt ataataaaga cggcaacaaa
      121 ttagatctgt atggcaaagt tgatggcctg cattattttt ctgataattc agcgaaagat
      181 ggcgaccaga gctatgcgcg tctgggtttt aaaggcgaaa cccaaattaa cgatcaactt
      241 actggctacg gtcaatggga atacaatatt caggcaaaca acactgaatc ttcaaaaaac
      301 cagtcatgga cccgtctggc atttgccgga ctgaaatttt cagattacgg ttctttcgat
      361 tacggacgta attatgggct ggaccgatat gctgcctgaa tttggcggtg actcttatac
      421 caatgcagac aactttatga ctggtcgtgc caatggcgtc gcgacttatc gtaatactga
      481 tttcttcggt ctggtaaatg gtctgaactt cgcggtgcag tatcaaggta acaacgaagg
      541 tgccagtaat ggtcaggaag gcaccaacaa cggacgtgat gttcgccatg aaaacggtga
      601 cggctggggt ctttccacaa catatgattt aggcatgggc tttagcgctg gtgcggcata
      661 cacctcttct gaccgcacca atgaccaggt taaccatact gcggcgggtg gtgataaagc
      721 agacgcgtgg actgctgggc taaaatacga tgctaacaat atttacctgg caaccatgta
      781 ttcagaaacg cgtaatatga ccccgtttgg cgacagcgat tatgctgttg caaacaaaac
      841 ccagaacttt gaagtcactg cacagtacca gtttgatttt ggtctgcgtc cggcagtctc
      901 tttcctgatg tctaaaggcc gtgacctgca cgctgccggt ggtgcagaca acccggcagg
      961 tgttgatgat aaagatctgg ttaaatacgc cgatgttggc gcgacttact atttcaataa
     1021 aaacatgtcc acttatgttg actataaaat caacctgttg gatgaagatg acagtttcta
     1081 cgctgccaac ggcatctcta ccgatgatat tgtcgcttta ggtctggttt atcagttcta
     1141 aatcctcctg cccgctgtta tggcgggctt tttctgctta ttcttcctcc tttgatctaa
     1201 attaaaaatg tgaactccgt cattacacaa aaagtgtcat ctggcgttac actttatgcg
     1261 gatactaaaa caggaggttt tatgaacaga acgattcttg tccctatcga tatttccgat
     1321 tcagaattaa ctcaacgcgt gattagccac gttgaggcag aggcaaagat tgatgatgca
     1381 gaggttcatt tcctgacggt aataccttca ctgccctact atgcctctct gggtttagcg
     1441 tattccgcag aattaccggc aatggatgac ctgaaagcgg aagccaaatc gcaactggaa
     1501 gagatcatta aaaaatttaa actgccaacc gacagagtgc atgtccatgt tgaggaaggc
     1561 tcgcccaaag accgcattct ggaattggcg aagaagatcc ccgctcatat gatcatcatt
     1621 gcttcccatc gaccggatat caccacttat ctgctcggtt ccaacgccgc agctgtagtg
     1681 cgtcacgcag agtgctccgt gctggttgtg cgctgacact aacgcccgca cattgctgcg
     1741 ggctttttga ttcatttcgc aaatgtgctg acattttccc ctctaatccg taccatacac
     1801 gccacagttt ttatatcaga tttcttatgc tgggcgttcc ggcatgacgc atactcttct
     1861 gatgccatat aacgaattga gtcgctttta aatgtcgcaa aatcaagaaa ttagcaagaa
     1921 agaacaatac aacctgaaca aattacaaaa acgcctgcgt cgtaacgtgg gcgaagccat
     1981 tgctgacttc aatatgattg aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa
     2041 agacagctat accatgctgg agattctgcg caatttgcag caaagcgcgc caatcaattt
     2101 ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc
     2161 cgagtatctt gaaaagctgg gcgttgagta caagattgtt gaagagaaca cttacggtat
     2221 cgtgaaagag aagattccag agggcaaaac cacttgctca ctgtgttctc ggctacgtcg
     2281 cggtatcctt tatcgcaccg caacggaact gggggcgacg aagatcgcgt tgggccacca
     2341 tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc tacggcggca agatgaaagg
     2401 tatgcctccg aaactgatga gcgatgatgg caaacatatc gttattcgtc cgctggccta
     2461 ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg
     2521 caacctgtgc ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga
     2581 ctgggataaa cgttatccag ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt
     2641 gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa ggcatcaccc acggttctga
     2701 agtggttaac gggggtgatc tggcgtttga tcgcgaagag atcccactac aaccggcggg
     2761 ctggcagcca gaagaagatg aaaatcagtt ggatgagtta cggctgaatg tggttgaagt
     2821 gaaataactc ggatagcgcc cgatgcgcaa acgtatcggg ctactctaat ggaggctgaa
     2881 taagacgcgg tcagcgtcgc atccgacaat cccgacgaag atgtttactt ttgcatcagg
     2941 caattcaaca ttttattatt ttaataaccg cacccggcac gtttttcctt taatcttccc
     3001 gccctgtaac tgtttccatg ctttatgagc aacagcctga cggaccgcga catagacatg
     3061 cgccggatgc acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag
     3121 tgcacctaat acatcacccg ggcgcatttt ggcttttttc ccgccatcga tacacaacgt
     3181 tgccatttct gcttccagcg tcacaatgga actattagct ggtggcgttt gccagttaag
     3241 ttttatctgc aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gagcacagaa
     3301 actgatcgcc agaccgctat ttcctgcacg agctgtacga ccgatgcgat gtacatgaac
     3361 ttcagggtcc cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc
     3421 acgcgcagca acatcagtcg cgaccagtac acgggcgcta ccgttagcaa aacgtaccag
     3481 ggtctgatca cgatcgcgtt gctccagatc gccgtgtaat gacaatgcac tttgccctac
     3541 ttcattcagc gcgtcgcaga cagcctggca atcttttttg gtattgcaaa acaccacgca
     3601 agaggatggc tgatgcaagc ttaataaccg ttgcaacaga ggaattttgc ctttgctgga
     3661 tgtctcataa aattgttgtt caatgggtgg caaagcatct gttgagtcaa tttcaatcgc
     3721 caaaggatcg cgttgcactc gtccgctgat tgcggcgatg gcttccggcc aggttgccga
     3781 aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa tggcatcgct
     3841 aaatcccata tccagcatgc ggtcggcctc atccatcacc aaagtattca gcgcatccag
     3901 tgataccgtg cctttttgca ggtgatccag caaacggccc ggcgttgcca cgataatatg
     3961 cggcgcatgt tgcaacgaat cacgctgcat accgaacggt tgaccaccgc acaacgtcaa
     4021 aattttggta tttggcagaa aacgcgccag ccgacgcaat tcacctgcca cctgatccgc
     4081 cagttcacgc gtaggacaca gcactaaagc ctgggtttga aatagcgacg catcaatttg
     4141 ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg ccagttttcg cctgcacgcg
     4201 aacatctttt ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg tcatggttaa
     4261 ataacccaac tcattaaggt tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa
     4321 agcggtcaca atctattctc gtggtcatcg acgcaaagtc atcaggcgcg tatcctcgca
     4381 gatctacgct cacgatgcga caatttaatc ggttcttcat cgggtggtgg gtcaggcatg
     4441 ggttgcgggc gagggatcgg atcgggcact ggaacaggat cgccaggaat cggttcaggg
     4501 acaggaattt gcaaataaat aagtgtcgtc atatttccct ccggtcattg ggtggactct
     4561 taaagggtag acgctgataa ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc
     4621 gtacgaatca attgtgctat gcagtaattc aaaaaaggaa gtaagacaat atggagcgca
     4681 acgcccatcg cttgacgttg cattcacctg caagagagat attgccctga atgggtagag
     4741 agtttattga cttcgctcaa actttgcggc gtttttgtat acagacagcc ggaaaaattg
     4801 cttttgttac aaccatttac tacgatgcaa ccataaagca acaccaccaa taagaacaac
     4861 taacagaata caaaaaattg aaaatccgaa ttgccacccg ccgccaggga tcccaccaag
     4921 gttgacgcca aataaccctg tcagaaaggt actgggtaaa aagaccattg ccatcaatga
     4981 cattgtatag gtacgacgag ctaaattttc ctgcatcacc tgagcgattt catccgccat
     5041 cacaccagtc cgtgctatac aggcgtcgat ttcgtcaagg ccgcgcccaa ggcgatcggc
     5101 aatatcctgc atccgacggc gttggtcatc gctcatccac ggcaaacgtt cactggcaag
     5161 acgagcataa acatcacgtt gcggtgccat atagcgacgc atcacaatta attgtttgcg
     5221 cagcagagcc aggaatccac gcggcggaat ttgctgatca aggagattat cttcaaggtc
     5281 gataatttta tcgtgcagct gctcgataaa ttcgctggaa tgatcggtca acgcatcgca
     5341 cacatccacc agccatcccc cgcaatcggt cggacccgtg ccctcttcca gatcactcac
     5401 cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat
     5461 atatacacgc atggcgacca gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg
     5521 caatgtaatc agcgtgcctt caccgagacg gctgactcgg ggccgtgtgc tctcgcccgc
     5581 cagcgcatca cgtacgttat tgggaagcag cggtgttgtc gccagccatt gggcgctatc
     5641 atggtgtaca taattaaggt ggagccagca gggatgcgct tcatcaatca catctgtatt
     5701 ttccagcggt ttaacgccgc ctctaccatc cagcatccag gcaaatactg catccgggac
     5761 attaacgtcc gatcccttaa tcgcttccac agtgcctcca tcatcaacgc attattttgt
     5821 agtctagcct tctggccctg ttacgcaaca tctcatcacc ccattaccct gaaatgatta
     5881 ataaaattct gtctaaattg aatacaaaaa
//
LOCUS       DAZKFZ010000252         5886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000252 DAZKFZ010000000
VERSION     DAZKFZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            147..794
                     /gene="fucA"
                     /locus_tag="ACHOQW_004318"
     CDS             147..794
                     /gene="fucA"
                     /locus_tag="ACHOQW_004318"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP9575272.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            822..1970
                     /gene="fucO"
                     /locus_tag="ACHOQW_004319"
     CDS             822..1970
                     /gene="fucO"
                     /locus_tag="ACHOQW_004319"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9575273.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2025..2780)
                     /gene="xni"
                     /locus_tag="ACHOQW_004320"
     CDS             complement(2025..2780)
                     /gene="xni"
                     /locus_tag="ACHOQW_004320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP9575274.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2892..4259)
                     /gene="sdaB"
                     /locus_tag="ACHOQW_004321"
     CDS             complement(2892..4259)
                     /gene="sdaB"
                     /locus_tag="ACHOQW_004321"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP9575275.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4317..5606)
                     /gene="sdaC"
                     /locus_tag="ACHOQW_004322"
     CDS             complement(4317..5606)
                     /gene="sdaC"
                     /locus_tag="ACHOQW_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP9575276.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1474 a         1470 c         1576 g         1366 t
ORIGIN      
        1 gattagttga actaggtcac aaaatataag cttgtgaatt tctttcttgt taatcaggaa
       61 gaagatggtg ggtaattaaa cggctaattc aatagtgtga aaggaacaac attattgccc
      121 tgttttgaat cagagagagg tagctaatgg aacgaaataa acttgctcgt cagattattg
      181 acacttgcct ggaaatgacc cgcctgggac tgaaccaggg gacagcgggg aacgtcagtg
      241 tacgttatca ggatgggatg ctgattacgc ctacaggcat tccatatgaa aaactgacgg
      301 agtcgcatat tgtctttatt gatggcaacg gtaaacatga ggaaggaaag ctcccctcaa
      361 gcgagtggcg tttccatatg gcggcctatc aaagcagacc ggatgccaac gcggttgttc
      421 acaatcatgc cgttcattgc acagcagttt ccattcttaa ccgaccgatc cccgctattc
      481 actacatgat tgcggcggct ggcggtaatt ctattccttg cgcgccttat gcgacctttg
      541 gaacacgcga actttctgaa catgttgcgc tggctctcaa aaatcgtaag gcaactttgt
      601 tacaacatca tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta tggctggcgc
      661 atgaagttga agtgctggcg caactttacc tgacgaccct ggcgattacg gacccggtgc
      721 cagtgctgag cgatgaagag attgccgtag tgctggagaa attcaaaacc tatgggttac
      781 ggattgaaga gtaatttcgt aaagcaacaa ggagaaggat gatggctaac agaatgattc
      841 tgaacgaaac ggcatggttt ggtcggggtg ctgttggggc tttaaccgat gaggtgaaac
      901 gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa tgcggcgtgg
      961 tggcgaaagt gaccgataag atggatgctg cagggctggc atgggcgatt tacgacggcg
     1021 tagtgcccaa cccaacaatt actgtcgtca aagaagggct cggtgtattc cagaatagcg
     1081 gcgcggatta cctgatcgct attggtggtg gttctccaca ggatacttgt aaagcgattg
     1141 gcattatcag caacaacccg gagtttgccg atgtgcgtag cctggaagga ctttccccga
     1201 ccaataaacc cagtgtaccg attctggcaa tccccaccac agcaggcact gcggcagaag
     1261 tgaccattaa ctacgtgatc actgacgaag aaaaacggcg caagtttgtt tgcgttgatc
     1321 cgcatgatat cccgcaggtg gcgtttattg acgctgacat gatggatggt atgcctccag
     1381 cgctgaaagc tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg tatattaccc
     1441 gtggcgcgtg ggcgctaacc gatgcactgc acattaaagc gattgaaatc attgctgggg
     1501 cgctgcgagg atcggttgct ggtgataagg atgccggaga agaaatggcg ctcgggcagt
     1561 atgttgcggg tatgggcttc tcgaatgtgg ggttagggtt ggtgcatggt atggcgcatc
     1621 cactgggcgc gttttataac actccacacg gtgttgcaaa cgccatcctg ttaccgcatg
     1681 tcatgcgtta taacgctgac tttaccggtg agaagtaccg cgatatcgcg cgcgttatgg
     1741 gcgtgaaagt ggaaggtatg agcctggaag aggcgcgtaa tgccgctgtt gaagcggtgt
     1801 ttgctctcaa ccgtgatgtc ggtattccgc cacatttgcg tgatgttggt gtacgcaagg
     1861 aagacattcc ggcactggcg caggcggcac tggatgatgt ttgtaccggt ggcaacccgc
     1921 gtgaagcaac gcttgaggat attgtagagc tttaccatac cgcctggtaa atgcgctgat
     1981 gtgataatgc cggatgcgac gtttgcggcg tatccggcac gccgttaccg taccaaccgc
     2041 aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat atcgcggcac
     2101 agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc aaccgcatcc
     2161 agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt ggcgcttttt
     2221 ggtccgattc ccgcaacacc cggtaccttt gaactgctga tccccgcaag tccccagtaa
     2281 tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc atccagccaa
     2341 cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca gtagccttta
     2401 tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc cagcgtggcg
     2461 gctaagtcat cggcttcgtt gccgctggtt gaccagcacg ggacgccgcg ttgctcaaag
     2521 gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg tcgacccgct
     2581 ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc gtcaaaaacg
     2641 gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg ctggcaggtt
     2701 tcgacacagg gcgacccctg aacggcatga atgcgacgaa taagattcag tgcatcgaca
     2761 ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg gtaacattcg
     2821 atactggaac aggctatggc tggatgagaa atcgggaaga ggcctcgcaa aacgaggcct
     2881 ctggagagcg attaatcgca ggcaacgatc ttcattgcca ggccgccgcg agaggtttcg
     2941 cggtacttgg cgttcatatc tttacctgtt tcgtacatgg tttcgataac tttatcgagg
     3001 cagacgcgcg gctcgctggt acggcgcagc gccatacgtg cggcgttcac cgcttttact
     3061 gccgcaatgg cgttacgctc gatgcatggc acctgtacct gtccggcgac cgggtcacac
     3121 gtcagaccga ggttgtgctc catggcgatt tccgccgcga tgcacacctg cgccgggctt
     3181 gcacctaaca gttctgccag accagccgcc gccattgagc acgccacacc aacttcaccc
     3241 tggcaaccca cttcggcacc agaaatcgac gcgttcatct tataaagaga accaatggcg
     3301 ctggctacca gcaggtaacg agccagtgag ttagcgttca cttcgcggat aaacttatcg
     3361 tagtacgcca gcactgccgg gataatcccg cacgcaccgt tagtcggcgc agtcaccacg
     3421 cgaccgccag cagcgttctc ttcgttcact gccagtgcaa acatgttgat ccagtcaaca
     3481 accgccatcg ggtcagtggt ggttttatcc tggctgacca gcatccggcg tagtgccgca
     3541 gcacggcgtg gaacacgcag tttgccaggt aacacacctt cggtggaaat accgcgctca
     3601 ataccgccac acatcacttc ccagacgttc gccaggtgct gttccagctc ttctttgctg
     3661 tgcagcgcca gctcgttttt catcatcagg ccagagagtg acagcccggt ttcctgacaa
     3721 tgtttttgca gatcggctgc tgaactgtac ggataaggaa cttcaaccgg tgcgctatcc
     3781 tgctggccaa aatgctcttc atcaacgata aagccaccgc caatagagta gtaagtctga
     3841 ctgtaaacga ctttatcacc cgccagcgcg gtaatgcgca taccgttttc atgcagagaa
     3901 aggttgtcgg catggaagtt catgcactga tcaaccggga actccacttc atgctgaccg
     3961 tttgccagca tcaggcgacc atgagtattc acatcctgaa taaaaccagg gatggaatcg
     4021 atatccacgg tatccggcag gttacccgcc aggcccataa taatggcgat atcagtgtgg
     4081 tggcctttac cagtcagaga gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct
     4141 ttaagcaggt tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt cattggtcca
     4201 acggtatgag aactggaagg gccaatgccg attttgaaaa tatcgaatac gctaatcata
     4261 ggaaatacat cgcgttaaaa cggaggaagc gccgcccgaa agcggcgcga aaggacttag
     4321 ctgaacagag agtagaagat tgcggagatt gcaatcagac ccatcacgac aacgaatacg
     4381 ttgctgatgt gaccgctgta cttacgcatt gccggtactt tctgaattgc gtacatcggc
     4441 atcaggaaca ggatcatcgc gatgattggg ccgcccaggg tttcaatcat acccaggatg
     4501 ctcgggttca gggtggcaac aatccaggtc gttaccagca tgaacagcgc agtgatacgg
     4561 ttcagcttgt tgatttcgat agacttacct ttaccacgca gagatttaat caccataccg
     4621 ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat agcgataatc
     4681 gcgataatcg gagccatcca cgcgataacc ggtgcgttaa agtggttagc caggtaagac
     4741 agaatcgaga tgttctgctc tttagccgca gccaggtctg ccggagtcag gctcagtacg
     4801 cagctgaaga cgaagaacat tacggtcagc accatcatga tgtgtgcgaa tgccaggatc
     4861 ttcgagcatt tctgttctgc catatcgccg tactcttcac gcttcgcaac ggcgaaagaa
     4921 gagatgatcg gagagtggtt gaacgagaac accattaccg gaattgccag ccacagggtc
     4981 atccacagac cgtttccggt tgcagatgca gtgtccagag acagcgtttc cagtgctgcg
     5041 ccgttccact gcgggatcag gtacagagcc agcagcatca gtacgccaac aaacgggaat
     5101 accagaatac tcatcgcttt aacgatcatc tgttcaccaa agcgaacgat ggtcatcata
     5161 cccacgatca ggatcagcga cagaatcgca cgcggcggtg gtgtcatacc cagctggtga
     5221 gacatgaagc tttcaactgt attggtgatt gccacgctat aaaccagcag gatcgggtag
     5281 atagcgaaga agtagagcag ggtaatcagt ttacctgcgc caataccaaa gtgttcttct
     5341 acaacctcgg taatgtcttc gcccgggttt ttaccagaca gtacgaagcg agtcaggccg
     5401 cggtgagcaa aaaacgtcat cgggaacgca aggatagcca tgatgatcag cgggatcata
     5461 ccgccaacac cggcgttgat tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa
     5521 aggcccagca tccacatggt gtctgtcttg cgccaggcac tacgagagtc tttcgacgca
     5581 atcgtgctgg tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa taattcagct
     5641 tttagccagt tcagcccggg aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga
     5701 tggcttaatc atttaccgcc gtaataattt gagggcggaa agatacacac aagttatgta
     5761 tctatcagtg atcttgatcc caaatacaat aatctacttg gaagatagat tatcccaacg
     5821 ctttactctg taaatcaaaa gtaaaagaga atttaagggc aatatttgcc cattaaacga
     5881 catctg
//
LOCUS       DAZKFZ010000253         5874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000253 DAZKFZ010000000
VERSION     DAZKFZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2384)
                     /locus_tag="ACHOQW_004323"
     CDS             complement(<1..2384)
                     /locus_tag="ACHOQW_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9575277.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
     gene            complement(2480..2668)
                     /locus_tag="ACHOQW_004324"
     CDS             complement(2480..2668)
                     /locus_tag="ACHOQW_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9575278.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(2665..2853)
                     /gene="dicB"
                     /locus_tag="ACHOQW_004325"
     CDS             complement(2665..2853)
                     /gene="dicB"
                     /locus_tag="ACHOQW_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9575279.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            complement(3334..3486)
                     /locus_tag="ACHOQW_004326"
     CDS             complement(3334..3486)
                     /locus_tag="ACHOQW_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9575280.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            complement(3761..4405)
                     /locus_tag="ACHOQW_004327"
     CDS             complement(3761..4405)
                     /locus_tag="ACHOQW_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9575281.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
     gene            4503..4730
                     /locus_tag="ACHOQW_004328"
     CDS             4503..4730
                     /locus_tag="ACHOQW_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9575282.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
     gene            4727..5152
                     /locus_tag="ACHOQW_004329"
     CDS             4727..5152
                     /locus_tag="ACHOQW_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9575283.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            5221..>5874
                     /locus_tag="ACHOQW_004330"
     CDS             5221..>5874
                     /locus_tag="ACHOQW_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP9575284.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     "
BASE COUNT         1417 a         1394 c         1412 g         1651 t
ORIGIN      
        1 cgttcacctt cgaatggaat agtcgttctt gcgtcgaaat ccatcaccag tcccagagca
       61 accatcgttc ttacatcgcg atcattggtg taacgccacg ggcaggggat cccctgccgt
      121 tcatatgaac ggcgtaaaat cacgttgtcg aaagttgcac cgttacccca gacctgaaca
      181 aaaaattcac cggagttttc gtcgataaat tcccggaatt gcagcagtgc atcatccaac
      241 gtgatttcat cggtcagaat ggaggattgt gcttcgcgtg actgtttcag ccaccactta
      301 atggtgtccc gatcgatgac cccacctgcg gtttccagat cgatagtttt gctgaattct
      361 ggccccatct ctccggttgc cggatcaaaa aacttaccgg ctatagagtt tattggcgca
      421 tcaggatttt ttcccattgt ttcaaggtca atcatcagat ggtgccacaa cctgctggtg
      481 gatgagattt catgatgacc gttcacttta attaagggat ttgctgtttc gccagtttta
      541 ttatcgctgg cgtgatactg atcgctgcca gtgttctcct tgtgcagatg ttcagtgcct
      601 tccatttcct ccggatcatt tttctgagct tcagcctgat tctcttcatc gaatgtttcc
      661 aggtaggttg cgttccccat caccgcacca cagtcaggac agttgccgcc accggtctga
      721 ccgcatgcgg tgcaaacttt ttccagttcc tgttgcactg ctggttctgg ttgttgttct
      781 tctggcccgt tttgttgcgt atccgggctg ttttgttccg cttctggctc attttgtttc
      841 acgttgggct gattctggtc ctcagcgtcg cgactctgga tccctttcac ccatttcgga
      901 tcattcgggt cgctaatccc ttcaacaaat tcaccacgtg atgcagcaag caatttatcg
      961 gcgtcaggct ggctgatatt ggctgcctgc ataattttgt ttacttcgtc agcggtaact
     1021 tttaccggct ctgattgttc ggaatcttca gcggtatcca cattttgcgg taagcccgtg
     1081 tatgtgccat tttttcgggc aaaatattct tcttttgaga tttcggtacc gccggcagcc
     1141 agcgccttgt ccagaccaga aagtttgttt gccctgccgt atttttcccc gcccttatcg
     1201 gtaaagacga aataaaatgg tccttcacgc tctacagatg gttcagcttc caccaggatt
     1261 tcattttttt gagtatcgga ttctgccgtc tccactggag cagtttgtgc tgctgacggc
     1321 tggagagcat cagtagagct ctggtctgtt tcttcatgtt caaacacgcc ctttgtcgtc
     1381 aggtattcac tgatatattt gttcagtgca acgggatctt tgtgaatatc gatcggacgt
     1441 tcgcgaacaa ggccaaaaat agtctgacgg tcatagccaa gtgcttcggg ctgtttgcgc
     1501 attgatgctg agatacgctt ccagtcttcg cgatccttgt cgataacctc atttgtcgcc
     1561 cagcgatgga tggtaccgtc aatgtttcct gcatccacat caccgggcca gagggcgcag
     1621 gccagttcat tgtcgagtgt tttccatgtc tgtttgtatt cgcggcgaat ggcaacagtg
     1681 acagccccgg ttttttcagc agcgttttca gtgttctgtt ggttgactct ggcgcgggtg
     1741 agatcaacaa cagacgtgta ttttccggtt tccttgcgtt caccttcgcg acgttttttc
     1801 cagatgcgca tctctgcctg aatttcaggc catttagcgc caggctcaca tttatgctta
     1861 acccacccga tggcatgcag tttaagttca ggatacatgg cgttaacttc tggcattttc
     1921 atcaacgctt caacgatatg tccgccgaat gttgccatgt cttcctgtag taattcctgt
     1981 gcgctaatca ccatatcaac ggtgatgttt tcgcatgtgt cgaatttaac caggactgcg
     2041 ttctgtactt caggggacag cttgtcaaaa ttgacgttca tcggatcgga ttctggttcg
     2101 accggtacaa aggaagctga ctcctcatcc cagcggtttt cctgcatata ttcggtatcc
     2161 caggaatcga gggcagggcg gggtatgccg ggtttatcct cgcagacaat aaatttataa
     2221 gcgcagtcct gagctgccgg gaattgctcc agaaattgcc agtgaaattt tgcgcgtgcg
     2281 cgacgctcat caccggcttc aatggcagtg gccaccgcaa cagcgctatc ttcttttatg
     2341 gcctgttcgt caggaatagc agcgcaaata aagattttac tcattgtgtt ttaacctcat
     2401 tacagatttc agggtgaacg aatccctgcc attgctggca tttttaatcc gttggtatgg
     2461 cgttaatatg gctggcgggt tatccagccg gtgtttcgtt attcaggtac agcgatactt
     2521 tttttaccgg taggcattca ccagaaattt tttgctcgtc tcttgcctgg aggcaggatt
     2581 ctttactggc ataaattccg gtaataacat tctgtgattc acccgttata agaaaaaccg
     2641 tcatcaccag tgcaaatgct gaagtcattg acgttctccg aaaataccaa gtttaataag
     2701 ggcaattcgg gaaagaatgg aattatcatt gagcagataa ggctcatatt tccgcatatt
     2761 aatggcatcc tcagtaaacg ctttattact gagcagaaca ccaatatcaa aacaaccttc
     2821 agacgtatta acgtttggta ataacgtttc cattatcgcg tcctcaacaa tgaattttgt
     2881 gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg tcgggttagt
     2941 tgatgctcgt tacgccagta aaagaccgcc ttactgcttt aactgttccg cgtgcgcata
     3001 gccgcattca ccgcatcaca aaattcactt taaaaagggc ggatatccat ttccgccgaa
     3061 tcaccagaaa agtgataaca gaggttgttg tggctggggt gtcacttaag cgtatggtca
     3121 acctgacaac ccggtgtcct caacggggga aggaataacc ccgccatact taccgccgcg
     3181 ccatttcgcg gattgccaca accagaagcg cacggtcgaa ttaaatttaa cgacgaccta
     3241 tacagagaga ctaacttcgc cgtgcgcttt cgtgttatgc cctgactttt cagggtaaat
     3301 taacctgtgg aaacctgttt ttactggcgc taatcagtta gcgtttcttg ctaaccagcg
     3361 atgcgcgcca gcttcggttt taaacgtttt acttctggta tacgtcatcg cggtaaacgt
     3421 gccgtcgtgg ttggggaata ctccgcacac cagagattca ttgttgccaa gattgagcat
     3481 atccatgttg acctcattta cccttaacgc cgggtcgcgg aactaaaaac ctgctgcgct
     3541 gttatacaaa gtgttcccgc cgtcatgttc atacgcctcg ggctggctac ttaacccctg
     3601 accactgccg ggtaactcga ggtattgccc gacgttctgt ggggcgaggt agggtggtat
     3661 gtaataaatc taaaagtatt tagtttttca gtcaaggtaa atctagatta atttaaaaaa
     3721 aaggtcggca ttgccgacct caagtaaagg acgtggcggg ttataagttg aactgtacac
     3781 cttttgctac ggcaacaatt ttacactctg gtgtaagtaa ggatgactga tagcgtggat
     3841 tgagagggct taaatacaca agcttcccat caataactaa tttctttata gtcatagatg
     3901 gttcattttt aagcggatcc ggaactatta cagcgacgat gctgccattt ttataacttt
     3961 ctcctggcct taggatcaca gtagcaccaa ctgggatact cggtgatcct gatgggttat
     4021 gcattgtgtc atcaggcatt gaaacggcaa aatcaccttc ctcgacatca aagaatgtgg
     4081 tgattctatc gacatttccc attgttttct ctccttttaa gattaggaaa gaaatcgcct
     4141 caccccacga aaggtagggg atcttggtgc ctgaggtact ttgtacaaaa gataattcag
     4201 gagacgatac tccatatagg agataggact cagtagttcc taatgcctgg gctaacttac
     4261 ttagagcttt gctacctggt tcgtttagat ctttctccca gtaccctata gtaaccccag
     4321 tcacgcctga aagcttaccc agttctactt gggtcagccc cttatctttt ctaagtctct
     4381 taagcctgaa gccaaggctt tccatcattt tctcctgcaa attgaatata aattatttta
     4441 gattgcattg acctaaaaaa aattatcttg taatctaaaa atgcttagat ttaggaggta
     4501 aaatgcgagt tgatgaactt gtccagtttt ttggttctgt tcagagggtt gccgattttt
     4561 atgggataac ccgcgaagct atttacatgt ggcgtaagcg tcctggtgaa atagttccga
     4621 aggggagggc tgcggaagct gctgcatact ccaaggggaa attatctttg aacccagaac
     4681 tttacaaaaa gaaggatacc actcagggcg aaggaaaggg ggattcatga aaatcaaaca
     4741 tgagcacatc cgcatggcga tcaatgcctg ggcctatccg gacggtgaga aagttcctgc
     4801 agctgaaata gcccggactt atttcgagct ggggatgacg ttcccggaac tgtacgacga
     4861 cagccatccg gaagccctgg ctcgtaatac ccagaaaatt ttccgttggc tggataaaga
     4921 cacccctgat gctgttgaaa aaattcaggc tctgttaccg gcgatcgaaa aggcaatgcc
     4981 gcctctgctg gtggcccgta tgcgcagtca cagctctgaa tattaccgtg agatcgtcga
     5041 acggagggat cggctggtga aagatgtgga tgattttgtc gcagcggcga tcgcctgggg
     5101 caccctgact aacagtggtg gtcagcctgg taatgctgtt gtcgtgcatt gaccaacaat
     5161 attcatgccg gatttcttcc ggatgttcga gggtaaagtt cggtatcaga tgaggtgagt
     5221 atggctaatg cctggctcag attgtggcat gacatgccaa atgatcccaa atggcgaacc
     5281 attgccaggg tctcaggaca gccaatcgca acagtgatgg cggtgtatat ccaccttctg
     5341 gtgagtgcgt cacgaaatgt cacgacatgt cacggcgtgt cactacgtgg tcacattgat
     5401 gtcacgacgg aagatttagc aagtgcgctt gatgtgacgg aagacgtgat tgattcaatt
     5461 ttgcatgcaa tgcaggggcg agttctggat ggtgatctta tttccggatg ggaaaaacgc
     5521 caggtgatga aggaggataa cggtaatgtt tcgcaaaccg caaaatcccc ggcagagcgc
     5581 aagagagcgc agcgggagcg ggaaagactg cggaagcaga acactgattg tcacgatgag
     5641 tcacgacgcg tcacgcatat gtcacgacaa atcacgacag atacagatac agataaagaa
     5701 ttaaacccca cacataacgc gcgcatgcgc gagagtgcta cgaccggtga gttgaatgac
     5761 gcgtcgttgc agacagccga acctgaatac ctggacggcc tgagcgaacc gatcgggaaa
     5821 tttccgatga ctggagtctg gcaaccgtcg ccggattttc gacagcgggc agca
//
LOCUS       DAZKFZ010000254         5833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000254 DAZKFZ010000000
VERSION     DAZKFZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2390)
                     /locus_tag="ACHOQW_004331"
     CDS             complement(<1..2390)
                     /locus_tag="ACHOQW_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9575285.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFASGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQYTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKT
                     LSEIQKQELLRNAALIDQQKIREQLRYREETLKNDNVAARASNESELLGYGQGERA"
     gene            complement(2443..2652)
                     /locus_tag="ACHOQW_004332"
     CDS             complement(2443..2652)
                     /locus_tag="ACHOQW_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP9575286.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            complement(2748..3122)
                     /locus_tag="ACHOQW_004333"
     CDS             complement(2748..3122)
                     /locus_tag="ACHOQW_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9575287.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(3128..3844)
                     /locus_tag="ACHOQW_004334"
     CDS             complement(3128..3844)
                     /locus_tag="ACHOQW_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9575288.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(3903..4247)
                     /locus_tag="ACHOQW_004335"
     CDS             complement(3903..4247)
                     /locus_tag="ACHOQW_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP9575289.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(4244..4690)
                     /locus_tag="ACHOQW_004336"
     CDS             complement(4244..4690)
                     /locus_tag="ACHOQW_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9575290.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4687..5037)
                     /locus_tag="ACHOQW_004337"
     CDS             complement(4687..5037)
                     /locus_tag="ACHOQW_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9575291.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(5047..5373)
                     /locus_tag="ACHOQW_004338"
     CDS             complement(5047..5373)
                     /locus_tag="ACHOQW_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9575292.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(5370..5833)
                     /locus_tag="ACHOQW_004339"
                     /pseudo
     CDS             complement(5370..5833)
                     /locus_tag="ACHOQW_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT         1189 a         1939 c         1383 g         1322 t
ORIGIN      
        1 ctggctcgtt ccccctgccc gtaccccagc agttcagatt catttgatgc acgcgcagcc
       61 acattatcat tcttcagggt ctcttcccgg taccgcaact gctcccggat tttttgctgg
      121 tcaatcaggg ccgcattgcg cagcagttcc tgcttctgta tctccgacag ggttttcagt
      181 tcacccagcg ctgtctggta cttcagcttc gccagctccg tgttctgacc ggccagtgcc
      241 agttgctctt tctgctgctt cagcagccgg gaaaaactgt cttccgcttt ttccgtctct
      301 gattttccac cccgggattt gggtttattc gcctcgttat tgcgccaggc ttccagggca
      361 ttactgatat aacgttgtct cgcctcctga tacggatccc ccacaaaacc gaggtcatcc
      421 gccgcatacc ccagtcggac gcgctctttt tcttcccctt tcagtctgga cagggccagc
      481 tcacgctctg tttttgtcag ggcactctgc tgtttatcat ccagagtggc ctgtggcagc
      541 cgtaacggca cattcaccag tccctgccgc tgctgaagca gttcattccc cagccccagc
      601 agacggttga attccgtata ctgaccgttc ataaccagca tggactggta caccttattc
      661 tgctctgccg cctgctgacg aattaacgcc acacgacggt cttccagccc ggcaagcaca
      721 tcctgaatgg actgcgcttt ttcctgcatc tgtgccagac gggactgctc aacggcaagc
      781 tgctctgttg cctgagcaag cccttccgtt acggtcttca ccgatgtcag atggtttatc
      841 atgaatccgt caccggtcgt ccagcccggg ttcgccagaa catactgata tcctgcgatt
      901 ttttcctgca gggatttcac ccgactggcc tgttcatcaa tcagccggtt ctgctctgtc
      961 agcgccgccc gtgttcgtcc ttcattatct gaggcttcag gcagagacat tgacggcgtt
     1021 ttatgcgcga tttcatctat cgtcagtgca tactggcgcg cagactccct ggcctgctcc
     1081 tgattctggt acagcgtgta ccatgctgca gcccccagca tcaccagtcc gggtacgcca
     1141 ccaaccagcc ccagcgcacc gctcatcaga cgtgagccca ccgccgttgt actgttcagc
     1201 gcattctggg cggcggttct ggcagcaata tttctgttca ggcgttcctg tgtggccgcc
     1261 agacgggcct ctgctgcaat ctgcatctcc gtcccgcggg ctgccgccac ggcctgctga
     1321 gcacggtaca cggctgccct tgcccgcgcc gtggcaatct gcgttcccct gaactgtgct
     1381 tccgccagtg caacttcatt acgtgcagcc gtcacaagtc ctgccgtggc agacatcgct
     1441 ccggaggcca tattgccaaa gtaacgtgca accccgacgg caaccagcgc gcccacggct
     1501 gttgccacat tatcaatctg tccggcaaca ccgttcagca tgccggagag cgtttttgtc
     1561 accccgctgg cctcattcgc accgcccacc caggccataa aggcgttttc caccttcgtg
     1621 atactactgg aaaccgtttc cggcatggcc gcatattcat cacgtaatat ccccagctgg
     1681 ctgattaacg cggggaccac tttatccgct gtcagttttc cgtcatccgc cattgccttc
     1741 agatctttac gggccacgcc catacccgca gccagtgcac gtacgatccg gtcaccactt
     1801 tcattgaccg aattaaattc ctcaccgcgc aacacaccct gtgccagcgc ctggctgaac
     1861 tgggtgatca ccgaacccgc ctcagccgta ctggcaccgg agattttcag ccccgtggaa
     1921 atggcctccg tcaccttcag tacatcaccg gcactgtaac catattcacg catcgaggca
     1981 gccgaacggg caaacagggc cgcattatcc gaaaatgccg tgcctgtccg ctggctgata
     2041 tccatcagca ctttctgtga tgacgaaaat tcatcggatg actgtgatgc ctgtttcaga
     2101 cgggcattta cggaactcca ctcatccgcc agtgaaatca ggtgtccgga ggcaaaggca
     2161 ccggcaaatg cgccagccat tccgacagca gaaccgcgaa tttccgtcaa ctggctgttc
     2221 agttctgcca gggcacgtcg ctgctcccgg gctgccgcag cggcctgacg cccgccattc
     2281 tgcagggtcc ggtaatattc actgcccata cgggacgccc gctggatctc cgactggaat
     2341 gactgtgaat ttgccgaaat tttgataatc agttcacgta acgtcgccat tcacctttct
     2401 ccgggcaaaa aaaaacctgc cacagcaggt tttcatcatt atttatgaca ttgctgcaag
     2461 gctcagcgcg tcttccagcg ccgcaaacgg atccacctcc ggcttatcct catcctcgcc
     2521 ccagcagagc atggcgtcct tcagtgcaac attcatcccc tgtgccccga aaaccgcttt
     2581 cacgatctgt gcattacgga tatccccgcg ctcatcaccc agcggggata ccctgtcgaa
     2641 ctccatccac atcatcgcct cgctcgcact caggctgtgc cgcagttcgg ataaggtgcg
     2701 ccccagacgg agcgcaagtc gcatcagaaa gcgaatttcc gggcgggcta cttttttctg
     2761 gccgactctg catcagcgat cagttccagt gcctgacgca gcaaccgggc atgtaccgga
     2821 ccatagacgg ccagcacctg ctcacggtcg tccggagtga acacccgttg caggtcagta
     2881 tcacacagga catcgcagaa cagcgtcaca tccgcttcca ggttacggcg ggttttcgcc
     2941 accaccgaca gggtatcgtc atcctctcca tcaccattga gcacttcctg ccacagatac
     3001 caggcctctg ccgaaggctc ccgcagcacc acgctgacat ttctccattc cggcaccttc
     3061 accgttttat gacggaaccc cgacagtctg gccagcgcca gtgttttcag atcttttgcc
     3121 ataagcctta tccgcccgca ccattaaccg ttactgtaca cgcatcagag gtaatgctct
     3181 gcggctgttc tgcagaatcc gttacctcgc aggtataagc ccccttatca cctgactgcg
     3241 tattggcttt actgaaagtg tcagtagtct gtccctctac cggctgacca tccttcttcc
     3301 aggcgtgttt ataaggcggc gttcccccgt tgacactgac tgacattgtc agcagcgcac
     3361 cggtattcac ggtaagtgtc ttctccagat ttttcacaaa cgccagcggt accacatagg
     3421 acaccggttt acccttcagg cgaagtgaaa acgttgcagc caccacgccg ttggtaccgg
     3481 atgaccaggt gtgctgacgc acttccgcca ggaacttaaa gcccttaccg gacggaaact
     3541 gcaccttaaa cgcatacacc gtgtcattgt cataggcatc acgcagggcg ttctgggcct
     3601 gattcagata aaaattaccc gacatggaaa tctcggacga cgcccccaga ccgttgatgt
     3661 tctcctgctc tgtggagcag agcgtggtca catcaatatc ctgtttctga ccggcggtga
     3721 actggacttc cttgatggtg cagtccaggc gcagatattc cgccttctcc atggtttcag
     3781 cagtcgccgg ggcagatgaa atcatcacct gcgtcagctg tgaacgttca tacaaagcag
     3841 acattctgcc tcctgataat aaaaaacccg cacgcggcgg gttatgggtt ctgttgaaaa
     3901 aattacaccg tgacctgaaa ctccagggtt gcacggtaac agcggttttc cggaatatag
     3961 tcctgcattt cactgacgga tcccggggcc agcagcatta tggcttcacg ggcgtcctga
     4021 cgtatctgac gcgcctgcgt cacagtcccg gcataaacgt ctatctgcac cgacactgag
     4081 gactccgcct gcccgcccat cacgtccgcc gacaccgatg aaatcaggct gaaaaccacc
     4141 cacggaagcg ccaccgacgg cctgccatcc agcaggggga ccacatacgg gtacacctgc
     4201 ccgccggcaa gatgcgccag atgaggatac aaatccgcct ccgtcatcgt ctcagtacct
     4261 catcaatggc ccggttcatc cgagcaatcg ccacctgtgc cgcctgttca ctgcgcacat
     4321 caaatgccgg gcgcacaaac gggtgcggtg gcatattcac ggtccccatt tccacaaacc
     4381 gccagtagaa agcattgcgc gggttatccg ccttcatggt gttatcgctg ttaccggtgt
     4441 ccggattaac accccggata tgcacaccgg attccatccc gccatcgcgg gagcaccggg
     4501 aaaggaccac cacattgcgg cgaagttttc ccctgcgtac cggtgcccgt gacaccactt
     4561 cttctttcag cacattcgca cccgcacggg ttgcctcacg cagcacccgg ttattttccg
     4621 caccactcag aagctgcaaa tcgcggctga tgtcctccag ccccgaaaaa tccagcaggg
     4681 tttcgatcat ttttcccctc ccagccgaca gagaatttcc agacgcccgc cggttgcatc
     4741 cggaaccggg accccgacga cattcaggac atggtcacgc cagggaccac tcagcacatg
     4801 aagtcgtgac gccgccgtga tttcacgacc agactgaccg cgtacccaga tgcggatttc
     4861 cgcctgcgcc atttccgcac cggactgcat ccgctcccgg ctgctcctgc cccggatatc
     4921 cgcatgaatt ttcccgcatg acacccattc ttccgtcatt tctccggcag cattacgggt
     4981 taacaccggg ttcagaacac tgatcatctg tgtcagacga cctgcagata ttgccattcc
     5041 ccctcctcat aacaccgtcg gacaacgcaa atcgtaaatc agcacggaaa cagaaaacgg
     5101 cagttccccc tgcacgaggt cttcccgctc agcaagatcc ggattccggt acagcatccc
     5161 ggtcagtcgc atggcagccc ccttcatccg ggttaatgcc tcaccaggga tcagctcacc
     5221 gtcctcacta atcactttat cccggctgcc ctggatgtag gccagcagca cggcggtagc
     5281 ctgacgaacc ttgtccatca gcatctcatc atccgcgtca tggtcaacac gcagatgtgc
     5341 cttgatttct tccagtgtca gtaatgctgt cactttccac ctcctgcatc ccgcccacgt
     5401 tttgcagcca gagtccagcc tgatgaatga gcttctccgg gcttatcacc ggtcatactg
     5461 ttgcagtgcc acaacgagcc ccccacgtca ccgtatcgcc ggggtggtaa gtttcgccga
     5521 ttctgaacac accacggtag agcatcaccg gcagggaaaa tgttttttcc gtacgctggc
     5581 cactgctctg ccggaccacc acagagaacg accgttcacc cgtcatgctg acgtcaatat
     5641 ccgccacccc gtcaaccagg cattcccatc cccgcatccc gtacgttttt tcatacgccc
     5701 gccagagtcc accctggtgt gtggcatacg agccccgggg aaaggatttt tgatcgtcaa
     5761 tggcggggag tatttccagt gccgtggcat cacgcccgtc ctgcggagcc ggcagggcac
     5821 tcaccgcctc cag
//
LOCUS       DAZKFZ010000255         5811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000255 DAZKFZ010000000
VERSION     DAZKFZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(88..375)
                     /locus_tag="ACHOQW_004340"
     CDS             complement(88..375)
                     /locus_tag="ACHOQW_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9575293.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(375..566)
                     /locus_tag="ACHOQW_004341"
     CDS             complement(375..566)
                     /locus_tag="ACHOQW_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9575294.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(594..995)
                     /locus_tag="ACHOQW_004342"
     CDS             complement(594..995)
                     /locus_tag="ACHOQW_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575295.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            1104..1376
                     /locus_tag="ACHOQW_004343"
     CDS             1104..1376
                     /locus_tag="ACHOQW_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9575296.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            1360..1785
                     /locus_tag="ACHOQW_004344"
     CDS             1360..1785
                     /locus_tag="ACHOQW_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9575297.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(1992..2447)
                     /locus_tag="ACHOQW_004345"
     CDS             complement(1992..2447)
                     /locus_tag="ACHOQW_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575298.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            2526..3617
                     /locus_tag="ACHOQW_004346"
     CDS             2526..3617
                     /locus_tag="ACHOQW_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9575299.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            3624..4370
                     /locus_tag="ACHOQW_004347"
     CDS             3624..4370
                     /locus_tag="ACHOQW_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9575300.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            4392..5162
                     /locus_tag="ACHOQW_004348"
     CDS             4392..5162
                     /locus_tag="ACHOQW_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9575301.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            5178..5591
                     /locus_tag="ACHOQW_004349"
     CDS             5178..5591
                     /locus_tag="ACHOQW_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9575302.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT         1577 a         1208 c         1616 g         1410 t
ORIGIN      
        1 ttaaaaactg tttttgtgtc aacagttttt aattgttgtt ttgggcaaaa aaatccctcg
       61 aaagagggag tatgaaaatt gttcaactca gatagagaag ggaaattgtc gccgagaatg
      121 tgtcacgctt acaatctcaa tgcttgaagc agctactctg tacaggatta tgtagttagg
      181 gtgggtcacg atctctctca atccagaaac ccgttcgctt ggtggatata agtacggatg
      241 ctcagataga ggtaataccg atgtttcaat gcgtattttt agtctacgtg ctgccggtgg
      301 gttctccttg gcgatgtaag ttatgatctg gcgcaaatca tcgcgagcag acggtagcca
      361 taaaatgggt aacattactc gctcttgtta gttacagcaa tttgagcaat aagattttcc
      421 atttcagcca ttacctcgtc atgtggaatt gcggggcgag tgtctgcaag gcttgacgtt
      481 actttagtgc gcaaccattc gttgtaactg ttttcttgtt cggtagtttc gaattctgaa
      541 actatcggag aaagggctgt acccatggca taactcctct tcttgtactg tggtcacgcc
      601 cggcggcttt tttgtgccgc cagccaccta gcaatggttt cttccattga ttttttcttg
      661 tctttgattt cttgaagcat tttttcttgg tcttcctcag gaaacgcact aaaagcttgg
      721 agcagttcgc gttggcgagg accaattttc atcgtgtcag gggtgaaaat ttgctcacac
      781 tcttcaggag gcaataaaaa ccaatgcaat ggatgccctg aaacctcaac cagtttatcc
      841 aaacttgagg ctttaggtgt agccttaccg ctgacccatt gttgaacagt ttgttgtgtc
      901 acaccaattc tacgggcaag ctcagcctgg ctccatccag tttcctgaag aagcttgctg
      961 attctgtaca tagatacttc tagggcgctc atcattattc aattttacag gtaaatactg
     1021 ttaaaagcat cacaataaaa aactgttgat tgcatacagt tttttattgt aggctttgct
     1081 tatagttttt agaggagggc aaaatgctag atagcactcg cgaaaaaatt aggcagaaat
     1141 acactcaggc tgaaataggt cgttatatgg gggtcgctca acagactgtt tggcaatggt
     1201 ttagctttgg cgttccccca aagcaggtaa ttccgttatg ccaactaatg aagtgggaag
     1261 ttaccccgca tgaaatccgc ccagatattt atcctaaccc aaccgacggt ttacctgttg
     1321 gatgtaaggt taacacatca aatgcgccgg agttgattca tgaaaatcaa gcatgaacac
     1381 atccgcatgg cgatgaatgc ctgggcgcat ccggacggcg aaaaagtacc ggctgcgaaa
     1441 attaccaaag cgtatttcga gctgggaatg acgttcccgg aactgtatga cgacagccat
     1501 ccggaagcca tggctcgcaa tactcagaaa attttccgct gggtggagaa agacactcct
     1561 gatgcggtta aaaaaattca ggcgttgtta ccagcgatcg aaaaagcgat gccgcctccg
     1621 ctggtggccc gaatgcgcag ccacagttcc gcttattttc gggagttggt agagacgaag
     1681 gaacggctgg tgaaagatat tgatgatttc gttgcatcag cgatcgttct gttcgatcag
     1741 atgaatcgtg gtggcccggc aggaaacact ctggctgtgc attaattggg taataaatat
     1801 gagtaatgac aaaaaattga cactgagcgt ttacgaaaac agtccgcaca tctggcgtgg
     1861 cggtttatct gatgtggagc tggcagagtg gttgatacat aaagctaatg cgctgctctg
     1921 gcgtttgtca gccagagaac agcgcaagga aaccagaata aagctggctg atgcagaagc
     1981 gtgtgccggg cttattgagg attatacaaa tcttggtatt tcttcagcag agagtgatcc
     2041 cattcagcct ctgagcaggg agtcaatcca gcacgctggt tgtatggcac atcttgtaac
     2101 tgctcgtcaa catgaggtgg gtattggatc acttccggcg ggatattcgc tgattccaga
     2161 gctggttgaa gcaagaaaat cagttcagaa aaagagagat gacgcacttc aattattgag
     2221 agagcactat ggcgcgatac cagaatgcga acagcgtcga taccctgaag gttatgaatg
     2281 gatgcagtct ctttttgaag ttcgctaatc aatatgtcga gacgaaggta tgtttcggcg
     2341 cgcagccagg ctctgtaatc cgggagcatt tcggggctgt tacaccagcg gtttgttgct
     2401 gcaacattta atacatgagc ctgataaagg cttttcaaaa aatacatgtc gaacctcctc
     2461 tggttctgtc gattgggaac cacagattat atccggagga aggttcggca ccagatgagg
     2521 tagccatgcg tgattacgca aaagtttctc cgcgattctg gctgggagaa acggggagag
     2581 aacttagaaa ggcgggtgca gaagcgcaag ttgttgcttt ttacctgatg acatcccctc
     2641 acgcaaatat gctgggtttg tattacctgc cagttttata ccttgctcat gaaaccgggc
     2701 ttggtctgga aggggcttca aaggggctta aaagggctgt tgaagctggt ttttgtagct
     2761 atgaccatga tgcagagatg gtctgggtcc atgaaatggc agcctggcag gttggggaaa
     2821 cgttgaagcc tggcgataac cgttgtgcag gtgtcaggaa tgagtatgca tcattacctg
     2881 aaaacgcttt tctgtcagtg ttttacgaca gatataaaac ggatttccat ctggatgtga
     2941 ggcggaataa tagccgaaat tcggtaaggg gcttcgaagg ggcttttaag gggcttcgaa
     3001 gccaagaaca ggaacaggag caggagaaag aacaggaaca ggacaaaaac actatggttc
     3061 atggcaaaaa aaacaccacg aaccaggcag gggatgttca gaccgtcaat cctggtcagc
     3121 cagcaggcac gacaccggaa gccgattcgg gcgctgtgca gcaggtgatg accgcagggt
     3181 cggagcaatc acaccaactg cagcagcctg aagccgattc cgccattcag cgggaagccg
     3241 atcgggtagt cccggaaagc accgggcagt ctgtgggacg agtggattat ccggatgtgt
     3301 tcgaacaggt ctggcgggaa tacccgttgc gtgctggggc aaacccgaag aaatccgctt
     3361 tcagtgcctg gaaggccaga ttgcgcgagg gggtgccacc agagaccatg ctggatggtg
     3421 tgaggcgtta cgcgagatac ctggcggcga ccgggaaagc gggaacggaa tttgttcagc
     3481 gagcgacgac gttttttggg ccggaccgga attttgaaaa cccctggttg ctcccggtaa
     3541 gcggcacgaa caaccagcgt tgtgtgaatc atatttctga accggatacc gaaattccgc
     3601 cgggattcag ggggtgatgt ggcatgaaaa acattgcggc agccggggtt cttgaacgta
     3661 ttcgcagact tgcaccacag gcgtcggttc caccgtaccg gacggtggag gagtggcggg
     3721 aatggcaact tgctgaagga cgaaaacgca gcgaggagat taaccgccag aatcaccagt
     3781 tgcgggtgga aaaaatcctg aatcgttcgg gcatccagcc tctgcacagc aaatgctcgt
     3841 ttgcgaatta tcaggtgcag aacgacgggc aaaaatacgc gctgagccag gccaaatcca
     3901 tagctgacga actgatgacc gggtgcacga attttgtgtt cagcggtaaa accggcaccg
     3961 ggaaaaatca ccttgcagcg gcgatgggta accggctgat ggcgaagggg cgcagcgtga
     4021 ttatcgtcac cgtgtctgat gtcatgagcg tgttgcatga cagctacgac aacggcaaat
     4081 ccggggaaaa atttttacag gagctttgca gtgttgattt gctggtcctg gatgaaatag
     4141 gcgttcagcg ggagacgaaa aacgagcagg tggtattaca ccagataatt gatcgccgga
     4201 cagcatcact gtgcagtgtc gggatgttaa caaacctgaa tcatgccgca atgagtacac
     4261 ttcttggtga gaggattatg gaccgcatga ccatgaacgg tggtcgatgg gtgacgttta
     4321 actgggatag ctggcgtcca aatgtcagca atatgagggt tgtgaagtaa ttttgtccgg
     4381 aggaaatttt aatggaaacc gtatctgacg cactgaaagc actgaaaaaa gcctcttcac
     4441 atgtggtggc agctcgcctt ggaatcagtc gtgaagaggc tgtcaacgag ctgtgggaac
     4501 tcaaaataaa tggcgtcgtt gataaaactg gtcacacctg gtttctggct ggcgaaggtg
     4561 aatcccgggt aaccgaagag cggccagtaa aatctgaagc acaggatatg ctgaccgggg
     4621 aggtcgaaca aaaagttacc gcagacatga tgattgagtt tatcggtcag gatggggcta
     4681 aaacgtgtga ggaactggcg ggtaagttcg gtgtcagtac tcgcaaggtt gcttccacgc
     4741 tggcggtggt aaccgcaacg gggcggctgg cacgcgttaa tcagaacggt aaatttcgtt
     4801 actgcatgcc gggcgataat ttaccagcag agccgaaagc cgcgctggta acggaaagtg
     4861 atggtaaggc ctttcctcag ccagcaggtg ctgcgttacc agtccgggaa gccgcaacac
     4921 aggaagaaat taaaacagaa actgtggcgg acattgtgca gccgttgcca tcgtttaccg
     4981 aaacgcaagc agatgagctg atttttccgt cccttcgcag ggcaaacctg gcgctgcgca
     5041 gggcgaaaag tgatgttcag aagtgggagc gagtctgcgc cgcgctgcgg gagctgaaca
     5101 agcaccggga tattgttcga cagattactg attcttcccg ccgtgttgta tcggaaaagt
     5161 gattgccgga ggcgcttatg gcaaaagtat ttacacagga agagcgggaa aaaattaaag
     5221 ggcaggttgt tgagctagta cgccggagtg ggcgcgagac gttacggcaa ctggaagcca
     5281 agacaggtgc gacaagatat ctgatgagcg ttcttgccag agagctggtt gccagtggcg
     5341 atgtatacaa ctctggttac gggttattcc catctgaaca ggctcgtaag gactggcaaa
     5401 acgcccgcaa aaaactatcg agggcaaaac tgaagaaacc ggttgtggtt gatcctgacc
     5461 ttatctggtc attaccagac ggagaaatac gccgctatga caggcaattg aacataattt
     5521 gtagtgagtg ccgtaacagt gaagtgatgc agcgagtttt gatattttac acaggagtaa
     5581 tgatggaata gtgaaaataa catgaaacct ttggattctg gtgtttcagt ggatgggaaa
     5641 agagcagata catagaaaat gaatagcaat aattcacagt ctggatgttg tttgtatgct
     5701 tataaaatga tcctagtatg cattgccgga gttgaatcag ctccaatgaa gaaactgaga
     5761 ggagtattta gaagatagct aaatggatgt aattattagt atctaaaaaa t
//
LOCUS       DAZKFZ010000256         5752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000256 DAZKFZ010000000
VERSION     DAZKFZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            210..773
                     /locus_tag="ACHOQW_004350"
     CDS             210..773
                     /locus_tag="ACHOQW_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575303.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            855..3494
                     /locus_tag="ACHOQW_004351"
     CDS             855..3494
                     /locus_tag="ACHOQW_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9575304.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            3517..4275
                     /locus_tag="ACHOQW_004352"
     CDS             3517..4275
                     /locus_tag="ACHOQW_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9575305.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            4286..4786
                     /locus_tag="ACHOQW_004353"
     CDS             4286..4786
                     /locus_tag="ACHOQW_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575306.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            4783..5253
                     /locus_tag="ACHOQW_004354"
     CDS             4783..5253
                     /locus_tag="ACHOQW_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575307.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            5250..>5752
                     /locus_tag="ACHOQW_004355"
     CDS             5250..>5752
                     /locus_tag="ACHOQW_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575308.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTV"
BASE COUNT         1481 a         1416 c         1500 g         1355 t
ORIGIN      
        1 taattcgctg ttaagtaaat ataatttgaa acaataaaga caaaatatca ccatccgccc
       61 acaccaaaga atatcttctt atgcgaccta ttttcttgtt agttagatcg gatagcttat
      121 tcaatggctg ggtataaata attgctattg actgaatcaa tgtattgcaa tgtttatatt
      181 tttaattatt tatggtttaa ggacaaataa tgagtaagtt tgtaaaaaca gctattgctg
      241 caacaatggt aatgggtgcg ttcgcttcta cttcaacaat cgccgctggc aacaatggta
      301 cagcacgttt ctacggcacc attgaagatt ccccgtgctc tatcgttcct gatgatcaca
      361 aactggaagt tgatatgggt gacattggtt cagggatcct gaaaaataac gggacttcta
      421 caccgaaagc tttccagatc catctgcaag actgtgtgtt tgacacccag acaacgatga
      481 ccactacctt caccggtaac gcgtcttcta ccaacagcgg caattactac accatttaca
      541 ataccgatac tggtgcggca tttaacaatg tcagcctggc cattggtgac gctcagggaa
      601 cctcttataa aagcggcgcg ggtatcgaac agaaaatcgt aaacgatacg gcgaccaaca
      661 aaggcaaagc gaagcagacg ctggacttta aagcctggct ggtgggcgct gctgatgcgc
      721 cagatttagg taattttgaa gccaacacga ccttccagat tacttatctc taagtgatac
      781 ccccatagct ttcgggctat ggggttttta aatggaattc gcgcaaatta taataattac
      841 ggatggatac gtgtatgcct gaccattctc ttttccgact gcgggtactt ccctgctgcg
      901 ttgcattggc aatgtccggg agttatgtca atgcctgggc tgaaaatgaa attcagtttg
      961 attcccgttt tctggagtta aaaggcgaca caaaaatcga tctgaagcga ttttccagcc
     1021 agggttatgt cgaacctggg aaatacaatt tacaggttca actaaataaa cagccgctga
     1081 cggaagaata cgatatttac tggtacgcct ctgagaacga tgccagtaaa acctatgcct
     1141 gcctgacgcc tgaactggtc gcgcagtttg gcttaaaaga ggatgtggca aaaaacctgc
     1201 aatggatcca cgacggcaaa tgcctgaaac ccggtcaact ggaaggcatt gatattaaag
     1261 ctgacctgag tcagtcagcg ttagtcattt cattacccca ggcttacctt gaatataccg
     1321 acatcaactg ggatccgcct tcacgctggg atgacggtat atctggttta attgctgact
     1381 acagtattac cgcccagaca cgacatgaag aaaatggcgg ggatgacagc aatgaaatta
     1441 gcggtaacgg gacggttggg gtgaacctcg gcgcatggcg tcttcgtgcc gactggcaga
     1501 ctgattattt gcatagtaaa agcaatgatg acgatgttat caacggtgat gacacgcaaa
     1561 aaaactggga gtggagccgc tactacgcct ggcgagcctt accgtcgcta aaagccaaac
     1621 ttggccttgg cgaagactac ctgaattctg atattttcga cggctttaac tacgtgggtg
     1681 gcagtatcag caccgacgat caaatgttgc cgccgaatct gcgcggctat gcgccggata
     1741 tctccggcgt ggcgcacacc accgcgaaag tgaccgtcag ccagttgggc cgcgtcatct
     1801 acgaaaccca ggtcccggcg gggccgttcc gcatccagga tcttggcgat tcggtctccg
     1861 gtacgctgca tatccgcatt gaagaacaga acggtcaggt gcaggaatat gacatcaaca
     1921 ccgcctcgat gccgttcctg actcgccccg gccaggtgcg ctataaactg atgatgggcc
     1981 gcccgcagga gtgggggcac cacgtggaag gcggtttctt ctccggcggc gaagcttcct
     2041 gggggattgc caacggctgg tcgctatacg gcggggcgct ggcagatgaa cactatcagt
     2101 cggcggcgct tggcgtcggt cgcgacctgt ctgtgtttgg tgcggtggcc tttgatatca
     2161 cccactcgca tacccgtctg gataaagaga ccgcctacgg gaaaggttca ctggacggca
     2221 actcgtttcg cctgagctat tccaaagact tcgatgaact gaacagccgc gtcacttttg
     2281 ccggataccg cttctcggaa gagaacttca tgaccatgag cgagtatctc gatgccagcg
     2341 acagcgaaat ggtgcgcacc ggcaacgaca aagagatgta caccgccacc tataaccaga
     2401 acttcaggga tgccggtgtg tctgtttatc tcaactacac ccgccatacc tactgggatc
     2461 gcgacgaaca gaccaactac aacgtcatgc tctcgcacta cttcaacctg ggcagtatcc
     2521 gcaacatgag catttccatg accggatacc gctacgagta tgacaaccag gccgataaag
     2581 gtgtgtacat atcgctcagt atgccgtggg gtgacagcag caccatcagc tataacggca
     2641 actacggcag cggttcggac agcagccagg tggggtattt cagccgtgtc gatgacgcaa
     2701 cccattacca gttgaacgta ggcaccagcg acaatcactc cagcgttgac ggttattaca
     2761 gccacgacgg atcgctggcg caggtcgatc tcagcgctaa ctaccatgaa gggcagtaca
     2821 cctcggcggg tatttcctta cagggcggcg cgacgctcac cgcacaaggt ggcgcgctcc
     2881 accgtaccca gaatatgggc ggtacgcgtc tgctgattga tgccgacggt gtggctggtg
     2941 ttccggtgga aggaaatggc gcggcggttt acaccaatat gttcggtaag gcagtggtgg
     3001 cagacgtcaa caactactac cgcaaccagg cgtatatcga cctaaacaac ctgccggaaa
     3061 acgccgaagc cacccagtcc gtggtgcagg ccacgcttac cgaaggggcc attggctacc
     3121 gtaagttctc ggtgatcagc gggcaaaaag cgatggcggt gctgcgtctg caagatggca
     3181 gttatccgcc gtttggcgcg gaagtgaaaa acgacagcgc gcagaacgtc ggtctggttg
     3241 acgatgacgg caacgtctac ctcgcgggcg taaaacctgg cgagcatatg atcgtttcat
     3301 ggggcggtgt ggcccactgc gatattcatc tgcctgaccc gctgccagcc gatctgttca
     3361 atggcctgtt attaccatgc cagcaaacag gggcgatatc tccttcgatg cctcatgaaa
     3421 ttaagccggt gatccaggag cagacccagc aggtgatgcc aacggaagcg ccagtatcgg
     3481 tatcagccaa ttaataacgt gattaaggaa tgatccatga cgaatctatt ttgctctgcc
     3541 agaacggccg caacgacgct ggcgttactg ataactgccg ccagcctgtc agtacaggcc
     3601 tctgtcacgc cggaccgtac acgcctgatt tttaacgaaa gtgataaatc aatcagcgtc
     3661 acgctgcgca ataacgaccc gaaaatgccg tatctggcac aaagctggat ggaagatgga
     3721 cagggtaaca ggattagctc gccactgacg gtcctgccgc cagtgcagcg catcgattcc
     3781 atgatgaacg gtcaggtgaa ggtacagggc atgccggata tcaacaagct gcctgccgac
     3841 cgcgagagcg tgttctattt caacgtgcgt gagatcccgc cgaaatcgaa caaacctaac
     3901 acgctacaga tagcgctgca gacgcgcatt aagttgttct ggcgaccaaa ggcgctggaa
     3961 aacgtcagca tgaaaaaccc gtggcagcat aaggtgacct taaccctcag tggtcaggag
     4021 tttaccgtca ataacccgac gccgtacttc gtggttatca gtaacgccag tgcgcagaaa
     4081 aatggcaatc cggcagcggg attttccccc atggtgatat cgccaaaaac ctctgtgccg
     4141 ttaaaagtca aaatggatag cgtcccggtg ctgacctatg tgaatgatta tggtgcgcgt
     4201 ttgccgctgt tcttccgctg tacgggaaat acctgtcagg tggacgaaga gcaaaacagc
     4261 aaaggccagg gataggagca atagcatgaa aggagcttat tcggcctggg cgtggttatt
     4321 gctgtgtagc attgtgagca cgcctgtaat ggcggggagt aaatcggttg cgatggtctt
     4381 gcgtgtgctc gttgatgccc cgccgccctg tacggtaacg ggtgcgagcg tggagtttgg
     4441 taatgtcttc atttccaaga tcgatggggt aagttacaaa cgccccattg actactcgct
     4501 tgtgtgcaac aatctggcaa tggacgacct gcgcttaaat atgcaggcaa ccacaaccgt
     4561 aattaacgga gaaacggtaa tcgatacggg tattgccggt tttggtatcc gtattcagaa
     4621 agtaagcgat cactccattt tggatttaac gcctggcgca tggttaccgt ttaacttttc
     4681 atctggcgca ctggctctgg aagcggttcc tgtcgtgcag agcggtgtat cgctaacggc
     4741 ggcggaattt agcgccagtg ccacaattgt ggtggattat caatgaaacg gatctctctc
     4801 ttaatgttgt ggagctttag ctttatggcg ctctcgaatg tctcttttca tggttattta
     4861 gtgcagccac ccaattgctc tattagtaat gggcaaaata tagagttgac gtttcgggac
     4921 gtgaatattg atgacattaa cggtagcaat tacgaacagg tcgtgccgta taccattacc
     4981 tgcgataccg ctgtccgtga ccctcaaatg gaaatgacgt tgacctggag tggtacgcag
     5041 agtgactttg acgattcggc cgtagccacg gatctcaatg gattaggaat acacctcaaa
     5101 caagccggga gcgattttaa gctgcatacg cccttagtgg tgaatgaaac atcccttccg
     5161 gtactaacgg cagtacccgt aaaaaagagc ggtgtcgact tacccgagtc agactttgaa
     5221 gcatgggcta cgttacaggt ggattaccaa tgaagaataa ccgtgcgtgg gcgcttatca
     5281 gtggtctgat attgttcagc ggaacggccc cagctgccga taacctgcat tttaccggta
     5341 atttgcttgg taaatcctgt actcctgtaa tcaatggcaa cttacttgca gaaattcatt
     5401 tccccacaat tgctgccagc gatttaatgc aacgtggtca gtcagatcgc gtaccgttag
     5461 tttttcagtt gaaagattgc aaaagcacca cggcgtttaa tgtcaaggtg accttgatgg
     5521 gaacagaaga taccgactta ccaggatttc tgtcgattga ttcgtcatct tctgcaacgg
     5581 gtgttgggat tggcattgaa actgccggag gggcggctgt acctattaac agtaccacag
     5641 gtgcctcatt tccattaaat cagggaaata acagtgtcaa ttttaatgcc tggttacaga
     5701 ccgtaaatgg acgaaatgtt acatcgggtg atttcaccgc cacaatgacg gt
//
LOCUS       DAZKFZ010000257         5730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000257 DAZKFZ010000000
VERSION     DAZKFZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(42..326)
                     /locus_tag="ACHOQW_004356"
     CDS             complement(42..326)
                     /locus_tag="ACHOQW_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9575309.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            604..3036
                     /gene="hsdR"
                     /locus_tag="ACHOQW_004357"
     CDS             604..3036
                     /gene="hsdR"
                     /locus_tag="ACHOQW_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFP9575310.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            3103..4572
                     /locus_tag="ACHOQW_004358"
     CDS             3103..4572
                     /locus_tag="ACHOQW_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9575311.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4572..>5730
                     /locus_tag="ACHOQW_004359"
     CDS             4572..>5730
                     /locus_tag="ACHOQW_004359"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9575312.1"
                     /translation="MSVEKLIVDHMKTWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPE"
BASE COUNT         1691 a         1371 c         1326 g         1342 t
ORIGIN      
        1 ataaacatca ataggcagaa ctcttttttg caaatcatct tttatcccct gaatcgggat
       61 gattttttta gctcggagac aataatgctt aacgttgtaa gacgttgttg tccatctaca
      121 acttcatact gattttgccc tttattgata caaatcatac tcccaataaa atatccattc
      181 tcttctgatt gagagctttt ataaatgtca tcaatgagtt gctctgcatt atctaccgtc
      241 caactgtaag gacgctgata agaaggaata ataaactttt tgttttcagt aaggatatcc
      301 tgcaccttac attctttagc ttccatttca tatacccaat caaacaatca acaataacat
      361 tatctttctt gctcgaaaga aacgcaacta taagattttt atatattttg ttagagttga
      421 tatggttcct gtaacttctg tttatctgtc tctgatatgc ccagattaat tcaagcaaga
      481 tataacagag atatcaatgt gatctctgct acgtacttta cgttgaaaca ccgccctaaa
      541 tcatagccag gctattgatt ttatgtacaa tcagaatcgt cctgataaaa gcgagaatac
      601 cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac caaatgcgtt
      661 atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca ggaggtcaaa
      721 ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac ggtaaaatct
      781 gctgacatcg tgctgtatca caaacctggc attcccttag ccgtgattga agcaaaagcc
      841 aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg cctgctggac
      901 gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc caccgcagcc
      961 gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc cgctgaactc
     1021 tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt gattactcag
     1081 gactattacg acgatggcag cggtaaatcg ccacgttatt accagcttca ggcaatcaac
     1141 aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat ggcgaccgga
     1201 acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc aaaaaataaa
     1261 aaacgcattt tgttccttgc cgatcgcaat attctggtcg accaaaccaa aaataatgat
     1321 ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga tcccgcttat
     1381 gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca aaaagcgttt
     1441 aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca tcgcggcagc
     1501 gcatccgaag acagcgcctg gcgagaaatc cttgattatt ttagttccgc cacccaaatt
     1561 ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta cttcggcgat
     1621 ccggtttacg tctactcact aaaagaaggg atcgaagacg gcttcctcgc cccttataaa
     1681 gttgtccgtg ttgatattga tgttgatctg caaggctggc gcccaaccaa agggcaaact
     1741 gacttaaacg gcgaagtgat cgacgatcgt atctataacc agaaagattt cgatcgcacg
     1801 atggtaatcg acgaacgcac agaactggtt gccagaacca ttaccgacta cctcaagcgt
     1861 accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc agaacgtatg
     1921 cgccgcgccc tggttaatct caacccggag caggtgaaaa agaacgacaa atacgtcatg
     1981 aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat caacccgaaa
     2041 aaaccgtacc cggttatcgc gaccacttca gagctgatga ccaccggtgt ggatgctaaa
     2101 acctgcaaac tggtagtact ggaccagaac atccagtcga tgaccaaatt caagcagatt
     2161 atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac catcctcgac
     2221 tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc cgaaaaagtc
     2281 atggatacca caccagagga tatcgccgat ccagaatctg attttgaaga gaaactcgaa
     2341 gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc tgcgccacca
     2401 taccaggtta aagataccga tgatgtcggc ccacttccgg aagaagacga gaagaaaatc
     2461 cgcaagtttc acgtcaacgg tgtagcagtg ggcgttattg cccagcgtgt tcagtattac
     2521 gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa aacactgctc
     2581 aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga tcgcaaagaa
     2641 gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc agaagaagtc
     2701 ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag tgtatggtca gccgccgtta
     2761 acccgcaaag agcgcgccga gaacgtgcgc aagcggaact acttcacaaa atactctgaa
     2821 gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg cgtacaggag
     2881 atcgaaagta ttcaggtgct gaaacttaag ccattcgaca gcatgggcac cttaccggag
     2941 attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag cgagctggaa
     3001 aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc gccttctgca
     3061 accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat cagctcagta
     3121 atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggatggcga tgcgcagcgt
     3181 ctcggtcagc tctcctggct gctgtttttg aaaatcttcg atgcccagga agaggcgctg
     3241 gaactggagc aggataacta tcaatatccg atcccacagc gttatttatg gcgcagttgg
     3301 gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa tgatgatctg
     3361 ttcccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg ctacgtggtg
     3421 aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact gcgtcaggtg
     3481 atcaataagc tgaacgaaat tgactttacc agcgccagcg aacgccatct gtttggtgat
     3541 atttacgaac agatccttaa agatctgcaa tctgcgggca atgcgggcga attctatact
     3601 ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg cgaatccatt
     3661 atggacccgg cctgcggtac gggcggtttt cttgcctgcg catttgatca tgtaaagaac
     3721 aaatacgtga agagcgtcgc cgatcatcag acgctgcaac aacagatcca cggtgttgag
     3781 aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctacacgg cattgaagtg
     3841 ccagtgcaaa ttcgtcacga caacactctg aacaaaccgc tttcctcccg ggatgagcaa
     3901 ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg aagaagacgg tattgagaag
     3961 aactttccgg cagagatgca aacccgcgaa acggcggatt tgttcctgca actgattgtg
     4021 gaagtactgg cgaaaaatgg tcgcgcggcg gtggtattgc cggatggcac actatttggc
     4081 gaaggcgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct gcataccatc
     4141 gtgcgcttac cgaatggtgt gtttaacccc tataccggca ttaaaaccaa cctgttgttc
     4201 tttaccaaag gtcagccaac caaagagatt tggttctatg agcatccgta tccggcgggc
     4261 gtaaaaaact acagcaaaac taagccgatg aagtttgaag agtttcaggc ggagatcgac
     4321 tggtggggta acgaggccga tggttttgcc agccgcgtag agaatgagca ggcgtggaaa
     4381 gtcagcattg atgacgtgat tgcgcgtaac ttcaatctgg atattaaaaa cccacatcag
     4441 gcggaaaccg tcagccatga tccggccgag ctgttagcgc agtatgcaaa acagcaggcg
     4501 gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct gtctggcaag
     4561 gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatgaaaacc tggacctcgg
     4621 cgttgcaaac ccgttccacc gccgggcgcg gcagttccgg aaaaattgat ttatatggca
     4681 ttaagaaatt acgtgagctg attctggaac tggctgtgcg cggtaaactg gtgccgcagg
     4741 acccgaacga tgaaccggcg tcggagctgc tgaagcgtat tgcggcagaa aaagcagagc
     4801 tggtgaagca aggtaaaatt aaaaagcaaa aaccactgcc ggaaattagt gaggaagaga
     4861 agccgtttga attgccggag ggatgggagt gggtgcgtat tagtgaaatt ggacatgact
     4921 ggggacaaaa aactccagat aaagatttta cttacattga tgttggttca ataaataaag
     4981 aatatggaat tattgaagag ctatctattc tttcagcgaa agatgcccca tcgcgagcga
     5041 gaaaaattgt tcagcaaggg actatcatat actctactgt tcgcccgtat ttgttaaata
     5101 tagctattat tgaaaacgaa atactacctg aaccgatcgc tagtactgcc ttcgctatta
     5161 ttcatccata tacggcgatg gatgctaatt tcatttatta ttatctacgt tctccggtat
     5221 ttgtttgtta tgttgaaaat tgtcagacag gggttgccta tccagcaatc aatgacaaac
     5281 aatttttttc tggaataact ccagttcctc catccttgga acaagttcgc atcgcaaaca
     5341 aaatcaaaga attaatgtcc ctctgcgacc aactggaaca gcaatccctg accagtctgg
     5401 acgcacatca acaactggtt gaaaccctgt tgggaacgct tacagacagc caaaccgccg
     5461 aggaactggc tgaaaactgg gcgcgtatta gcgagtattt cgacactcta tttaccaccg
     5521 aagccagcgt ggatgcgtta aaacagacca ttctgcaact ggccgtaatg ggcaaacttg
     5581 tgccgcagga tccgaatgat gaaccagcct ctgaactgct caaacgaatt gcgcaggaaa
     5641 aagctcaact ggtgaaagaa ggaaaaataa aaaaacaaaa accgttgccg ccaattagcg
     5701 atgaggaaaa accgtttgaa cttccggaag
//
LOCUS       DAZKFZ010000258         5693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000258 DAZKFZ010000000
VERSION     DAZKFZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            198..1748
                     /locus_tag="ACHOQW_004360"
     CDS             198..1748
                     /locus_tag="ACHOQW_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFP9575313.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            1774..2226
                     /locus_tag="ACHOQW_004361"
     CDS             1774..2226
                     /locus_tag="ACHOQW_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9575314.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPVAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2378..3652
                     /locus_tag="ACHOQW_004362"
     CDS             2378..3652
                     /locus_tag="ACHOQW_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9575315.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3645..>5693
                     /gene="mchF"
                     /locus_tag="ACHOQW_004363"
     CDS             3645..>5693
                     /gene="mchF"
                     /locus_tag="ACHOQW_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFP9575316.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     A"
BASE COUNT         1624 a         1069 c         1398 g         1602 t
ORIGIN      
        1 agaagatcca tttataagtg tgtttttatg ttcaacactt tactgatgaa gatatgattt
       61 acagtcgttg ataaataatt ttttaattta tgtgtaatta atagagtttt actgtaataa
      121 ggaagaagct gtcaggacac ttacttgctc ttttatgaac actcttttat gaaaattgaa
      181 acggagaata ctgccagatg agtcatcagt gttcactttc tgaactgaat gaaaacctgg
      241 tgcctttcac tgccaggcag atcaaggcct cattaatctg gtgtgcagag gatgtcagaa
      301 atccagacga actgcaaaat gcctgcagtt atattatcga tcctggcagt acagcttctg
      361 ccaaagtgtt ccatgcagag cgctatggtg gcagtggtat tcagcgtaat ggaggtggtg
      421 cacgttgtgg gtttgatggt aactaccagg ttaaaggaat aggaagtaat ccgttggttg
      481 gcgaaggtac tgacgaacgt cattctaatg gtgcactcgg cgctgttcat gcaatatatg
      541 aggctttgtg gggagaagta ctggctcaaa tattacctta tagtgctgtg cgggttcggg
      601 cggttttact tacagatctc tatactgaaa aggcatttga gcgctccggt aggaaatcac
      661 gaagagccct gttggtacgt gagcctgttg ttcgtccggc gcattttgaa cgggcacctt
      721 acttccaagt aaaaccggag tattccagtc tgttaattca cgatgcctgt cgggttagat
      781 ctgtgatcca caagctgcca ggatatctac ctgtaccacc ggaagaaatt gatgctgaag
      841 cacgaactga tccccggatt tattgcattg aggggttatg tgaactggca cgtcgtgagg
      901 cctggcaaat ggcattttgt cgaacacgtt tcctgagatt gacaacttct ccttctaata
      961 ttgcaatgga tggcagatta atggatttta acggactcag ttgtctgttt ccgggagatt
     1021 ccccagctga ttttgggtat aaactaagat tagctgaact ggcaaaagaa ccgatggtac
     1081 ttatgcaagg gctgtctgat ctctgcttgt atatcggaaa atatatgttt gaccctgact
     1141 tcactcttgc agcccgtttg aaggttgagg agatatttca gaaaactttt catgaagcat
     1201 gttattactg ttatctagaa ctgttgggta ttcctggaga atttataaca caaaaagaga
     1261 tacctgatat attgaaagaa ctggttaaca gttttgttgc attactcaat caatactgcg
     1321 agaaatcaca tgcccaagat attgtcaatc aggatggttc accattgcaa aagttggttg
     1381 tgacgctaat ccatcatagg cataatcaaa agcaggcact gaatagtagc atcaagaatg
     1441 atgtttattt caccgttgca caacagtgtt tttcccaggc catccactgg ctgacgcaag
     1501 gcagtaccag acgtcagata aatgcctcat tactcctgaa agaaattgaa catcatacca
     1561 tgaaaaggct gcaacccagg gaagagctga ggaaagaaaa tatgtgcgaa aaaattgcca
     1621 tcctgctgga taatcatggc gatgatcccc tttttttaca agaagcaatt tctgatatga
     1681 aaaattttat gcttaagttt tccagagatg catttggata tcttgaaccg ataagaaaca
     1741 cagtgtaaaa aatatataag ggaaatagta atcatgtctt atataaggga aaccatcaga
     1801 ggaaaagatg aatggactgt ttatgaacag atcggttttg cggtcagttg tatgctctac
     1861 aatcgtaatt acagtctgta tccggtgtta accattcaat actggactga atatgcgata
     1921 cagcataatc agattaaatt cctgtttgat tcacgaggtt ttccagtggc gtatattacc
     1981 tgggcatatc ttgaggctga tacagaagcg cgcctgctca gggatccaga attcaggttg
     2041 catccgtctg aatggaatga agatggaagg atttggattc tggatttctg ttgtaaacca
     2101 ggctttggtc gaaaagttat tgactatctc atacagcttc agccatgggg agaaggagaa
     2161 gtacgatggt taagcaggcg aaagaaaatt gtgacataca tccctgagcg gctgcataaa
     2221 acgtagtacc tctgaagata cagagataat tgtaaattac ggggtaaatg catcgctgat
     2281 actattttgc aggactctgt attttctgtc tttgattata gcatttcgct taaccagtga
     2341 aggtattaca ggaaaagtgt gagtaagagg agctattttg tttcgtcagg atgctttaga
     2401 aaacagaaaa atgaagtggc agggacgggc aatattactt cccggaatac cactatggtt
     2461 aatcatgctg ggaagcatag tgtttattac ggcatttctg atgttcatta ttgttggtac
     2521 ctatagccgc cgtgttaatg tcagtggtga ggtcacaacc tggccaagag ctgtcaatat
     2581 atattcaggt gtacagggat ttgttgtcag gcagtttgtt catgaaggac agttgataaa
     2641 aaaaggggat cctgtttatc tgattgacat cagtaaaagt acacgtagtg gtattgtcac
     2701 tgataatcat cgtcgggata tagaaaacca gctggttcgt gtggacaaca ttatttcccg
     2761 tctggaagaa agtaaaaaaa taacgctaga taccctggaa aaacaacgcc tgcaatacac
     2821 agatgcgttt cgtcgctcat cagatattat acagcgtgca gaggaaggga taaaaataat
     2881 gaaaaacaat atggagaatt acagaaacta tcaggcaaaa gggctgatta ataaagatca
     2941 gttaactaac caggtggcat tatattatca gcaacaaaac aatcttctca gcctgagcgg
     3001 acagaacgaa cagaatgccc tgcagataac cactctggag agtcagattc agactcaggc
     3061 tgcagatttt gataaccgta tctaccagat ggaactgcaa cggtacgagt tacagaaaga
     3121 actggttaac actgatgtgg agggcgaaat cattatccgg gcgttgactg acgggaaagt
     3181 tgactccctg agtgtcactg tcgggcaaat ggtcaatacc ggagacagcc ttctgcaggt
     3241 tattcctgag aacattgaaa actattatct tattctctgg gtcccgaatg atgctgttcc
     3301 ttatatttcg gctggtgaca aagtgaatat tcgttatgaa gcctttccgg cagaaaaatt
     3361 tgggcagttc tctgctacgg ttaaaactat atccaggact cctgcgtcaa cacaggaaat
     3421 gttgacctat aagggagcac cacagaatac gccgggcgac tctgttccct ggtataaagt
     3481 cattgcgatg cctgaaaagc agattatcag atatgacgaa aaatacctcc ctctggaaaa
     3541 tggaatgaaa gccgaaagta cactatttct ggaaaaaagg cgtatttacc agtggatgct
     3601 ttctcctttc tatgacatga aacacagtgc aacaggaccg ctcaatgact aacgggaatt
     3661 tcagacaaat tataaatcag cttgatatgc gctggcgacg tcgtgttccg gttattcatc
     3721 agacggagac cgctgaatgt ggactggcct gcctggcaat gatatgcggt cattttggta
     3781 agaatattga cctgatatct cttcgccgga agtttaatct ctcggcccgt ggagcaaacc
     3841 ttgcaggaat caatggaata gcggagcagc tggggatggt cacccgggct ctttcactgg
     3901 agctggatga acttggtgcc ctcaaaatgc cgtgtattct ccactgggat ttcagtcact
     3961 tcgtcgtgct ggtcagcgta aagcgtaacc gttatgtact gcatgatccg gccagaggca
     4021 gaagatatct cggtcgggag gaaatgagcc ggtattttac gggcattgca cttgaggtct
     4081 ggcccggaag tgaattcctg gcggaaaccc agcagacccg cataagtctc cgttcactga
     4141 ttaacagtat ttacggtatt aaaagaacac tggcgaaaat tttctgtctg tcagttgtaa
     4201 ttgaagcaat caatctggta atgccggtgg gaactcagct ggttatggat catgcgattc
     4261 cggcggggga cagagggctg ctgacgctta tttctgctgg cctgatgttc tttatattgc
     4321 tcagggccgc ggtgagtatg ctgcgtgcat ggtcctcact ggttatgagc acgctcatca
     4381 atatacagtg gcagtcgggt ctgtttaacc atcttctcag actgccgctg gcgttttttg
     4441 aacgccgtaa attaggtgat atccagtcgc gttttggctc ccttgacact ttgagggcca
     4501 cctttaccac ctgtgttgtt ggggcaatca tggacagtat tatggttgtg ggtgtttttg
     4561 tgatgatgct gttatatgga ggatatctta cctggatagt gctcggtttt accatggttt
     4621 acgttcttat tcgtctggtg acatacggct attaccggca aatatcggaa gaaactcttg
     4681 tcaggggggc ccgggccagc tcctatttta tggaaagcct gtatggtatt gccacggtaa
     4741 aaatccaggg tatggctggg attcggggaa cacactggct taacctgaaa atagatgcga
     4801 tcaattcagg tattaagtta accaggatgg atttgctctt cggggggata aatacttttg
     4861 ttgccgcctg tgatcaggtg gcgattttat ggctgggtgc aagccttgtg atcgataatc
     4921 agatgacaat agggatgttt gtggcatttg gttcttttcg tgggcagttt tcggatcggg
     4981 ttgcttcgct gaccagtttt cttcttcaac tgagaataat gagtctgcat aatgagcgca
     5041 ttgcagatat tgcactacat gaaaaggaag aaaagaaacc ggaaattgaa atcgttgctg
     5101 acatgagccc ggtttcactg gaaaccactg atttaagcta ccggtatgac agccagtcag
     5161 cacaggtatt cagtggtctg aatttgtctg tggctccggg agaaagtgtg gctataactg
     5221 gtgcctccgg tgccggaaaa accacattaa tgaaagtatt atgtggactg tttgaaccag
     5281 atagtggaaa agtactggtt aatggcacgg atatacgtca acttggaata aataattatc
     5341 accgtatgat agcctgtgtt atgcaggacg accgactatt ttcaggatca attcgtgaaa
     5401 atatctgtgg gtttgcagaa gaaacagacg acgaatggat gacagaatgt gccaaagcaa
     5461 gtcatattca tgatgtgata atgaaaatgc caatggggta tgaaacgtta ataggtgaac
     5521 tgggggaagg tctttccggc ggtcaaaaac agcgtatatt cattgcccga gctttatacc
     5581 ggaaacctgg aatattattt atggatgagg ctacaagttc tcttgataca gaaagtgaac
     5641 gtttcgtgaa tgctgctata aaaaagatga atatcacccg ggtgattatt gca
//
LOCUS       DAZKFZ010000259         5670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000259 DAZKFZ010000000
VERSION     DAZKFZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(255..575)
                     /locus_tag="ACHOQW_004364"
                     /pseudo
     CDS             complement(255..575)
                     /locus_tag="ACHOQW_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(998..1573)
                     /locus_tag="ACHOQW_004365"
     CDS             complement(998..1573)
                     /locus_tag="ACHOQW_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9575317.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(1642..2220)
                     /gene="terD"
                     /locus_tag="ACHOQW_004366"
     CDS             complement(1642..2220)
                     /gene="terD"
                     /locus_tag="ACHOQW_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFP9575318.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(2269..3309)
                     /gene="terC"
                     /locus_tag="ACHOQW_004367"
     CDS             complement(2269..3309)
                     /gene="terC"
                     /locus_tag="ACHOQW_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFP9575319.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(3332..3787)
                     /locus_tag="ACHOQW_004368"
     CDS             complement(3332..3787)
                     /locus_tag="ACHOQW_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9575320.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(3810..4967)
                     /locus_tag="ACHOQW_004369"
     CDS             complement(3810..4967)
                     /locus_tag="ACHOQW_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9575321.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4967..5548)
                     /locus_tag="ACHOQW_004370"
     CDS             complement(4967..5548)
                     /locus_tag="ACHOQW_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9575322.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1430 a         1515 c         1345 g         1380 t
ORIGIN      
        1 tccgttaagt tttgcagcta ccgatcaggc tgtacatcac tgccgcacta tcgctcgtca
       61 tctcaaagtc cggtctcgtc agcaggaagg tatcattctc tcccgccatt tttccagggg
      121 cccggtcaga taagtccctt tgtctatcgc tgactcctga ctcataaccc ggttagcaga
      181 atgcagggtc accacctcgc cacgaccaaa tccaaataag tcaattgcac cttctcaatc
      241 gccattttgt cagtaagcgt acagcctgaa ctgatggtat cttcaccatc aatgacaacg
      301 gtgatcgcaa ttttactgac gttcgccgga acacgatcca gtgctatctc aatgctggcc
      361 tgctgcgaac cggtaacgag cctgactgcc ccctcaggag aagacaaatt attataaaag
      421 ataaagtcag aatcgccact gacctttccc tgagcattaa gcatgaacag gcaggtatcg
      481 ggttcgcctt taaagccgga tttggcaggg tactgaagat tcagcctgat cgtagattgc
      541 tgaaggggta tgttttgtcc ggattgtaaa ttcatattaa ctctcctgat ttttgattat
      601 tattaatgcg cagcgtttat atatgttccc taggcttagt tctggacgct ggatattcgg
      661 tgaggcgtaa atatggtatg acaccatttt tcataacgct gaagtttcta tacctgttga
      721 atttgaattt tcattgaccg ggtatcttat tttccagggc ccgttccttc ataagtcgca
      781 aaagtaacat atatccgaag ggcatgctgt tgatatcaga cacggaatac tggctttaac
      841 cagcaaccac agataaaccc ggggcctgca taaaaggttc atgccagaat taacatagct
      901 ctgctttctg ccccccccct ttcccatatt cacccgttga tagcggatcg atacccaaaa
      961 aaaaccccgg cattgccggg gtcagactca gcgtctttta gacattgacg ccgtgctggg
     1021 aagcaagcgc tgacagacca ccggcaaaac cctggccaac agctttaaac ttccactcag
     1081 taccatggcg atacagttca ccgaagacca ttgcggtttc ggttgaggcg tcttcagaca
     1141 gatcgaaacg ggcaatttcc gtcccgttgt cgttgttgta aacgcgcatg aagctgttgc
     1201 tcaccatgcc gaagttttgt ttacgcgctt ctgcatcata gatggtaacg gcaaatacca
     1261 gttttttgat gtctgctgag actttggtca gatcgatttt gacctgctca tcgtcgccgt
     1321 cgccttcacc ggtacggttg tcgccctggt gctctactgc gccatcaggg ctggttttat
     1381 tattgaagaa aatgaaatgg gcatctgaca gtactttacc gtcttcacct actgcgaata
     1441 cggaagcgtc cagatcaaaa ccctgaccat cggttacacg ggcatcccag cccaggccaa
     1501 ccatagcgac attcatggtt ggtgcttctt tggtcagaga tacgttgccg ccttttacga
     1561 gagaaactgc catttttagc tcctgcaaac agttgaatga ggctgaatta acagccccag
     1621 atatgaaaag ttacttttcg atcaggacgc gttaatgccg tactgagcac atacagatgc
     1681 cagaccacca gcataaccct ggcctactgc gcggaatttc cactcaccat tgtggcgata
     1741 cagctcgccg aacagcatgg cagtctcagt ggacgcatct tcggtcagat cgtagcgagc
     1801 gacttcagtc tggttatcgt cattaaccag acgaataaac gcaccggata cctgaccaaa
     1861 gctctggcga cgagcctgag catcgtggat ggtcacaacg aagatgatct tgtcaacttc
     1921 agacgggacg gcgtccagtt taattttcag cgattcatca tcaccatcgc cctcaccggt
     1981 gcggttatcg ccggtgtgcg ttacggaacc gtcggatgac gtcaggttgt tatagaagat
     2041 gaaatctgaa tcgccgcgca ctttgccgtt tgaggccagc aggaatgctg aagcatccag
     2101 gtcaaagtcc tgaccgtctg ttgaacgcgc atcccagcca aggcccacca ggacattttt
     2161 cattgacgga gctgctttac tcagggagac gttcccgcct ttggaaagag aaacactcat
     2221 aaaataacct cttcgattag taattgttca ggttaacact taaggggatt aactcccctt
     2281 ttcctcagat tcaggtttgc ccgggaacat gacgcttgcg agaatgccca gcgccagtac
     2341 acccagtaca acatacaggc tggttgttgc cgcgatgctg taaccatgat gccagatgtg
     2401 atcggtcgca ttcaggccga gttttgccgc gatgaagaac agcagcacga taacggcctt
     2461 ctccagatga accaggtact gtttcagtgc ctcaaggaca aaatacagag tacgcagacc
     2521 caggatagca aacatcatgg cactatagac gataagcggt tcacgactga cggcaatgat
     2581 tgccggtacc gagtcaaacg cgaacattac gtccgagagt tcaaccacag ccacacacag
     2641 gaacagcggg gtcgcataca gcgccgcttt tttaccacgg ccaatggtga catcgctgtt
     2701 ttctggtttc gccagttcag catccacttc cttctggtta agtaggaagg catgccctct
     2761 gagcttcggc cagataggga agaagcgttt aaccatacgg taagccagat gctgggagta
     2821 atcctcaatt tcatcatcgt catcaccgct tttaagcatc atgaccgccg tccaggcaac
     2881 gataatagcg aagacaactt caacgtacgg ccccagactc agcaggctcg taccgatggc
     2941 gacaaagatg cccctgaaga cgatggcacc aatgatcccc cagtagagaa cacggtggcg
     3001 ataacgatcc ggaacggcga accaggagaa gattgccatc atgacgaaca ggttatcgac
     3061 cgacagcact ttctccagcg cataacccgt gacaaacaga ctggcaacct cagcaccgtg
     3121 gtggatatag aggaaaccgg caaatgccat cgcaaccaca acccagaata cggaccagag
     3181 cgcagcactc ttcagcgaga ttggcttgtc atcacggtgc ataaacaggt caataaagat
     3241 ggcaccgact gaaagcgcaa taaaaacaat gaccgtttca gtcgggaagc cgatgtgtgt
     3301 ggaaaccata ttttaccctt agggatgtgg atcagaggcc aaattcagcc gggtcgaagc
     3361 ccaacgcgat agcgatctcg cgggaggcca gtttctcgtc ctgatcgaag ttaccgtcac
     3421 ttttcgcaac ggcaatacca acacgtaagg ccagctgagc ggcctcaggc tgatctttca
     3481 gcgccaggat gtatttcatg gtttcgccct tgccaatttc aacatcgaaa tcgaagcttg
     3541 aaaccagttt attgaagaac tcaatcacct cattggtatc gaagaccttc agctcttctg
     3601 aagagcgcag aaagcccatc attttctgct tttcttccga acttacgccg tcactcgata
     3661 cggcaatacg ggcacataca gcaacggtgc cctgcatgaa ttttttgttt ttgaaacggc
     3721 caacctggcg ggtcagttcg tcacggcctg agttaatggc acctttaact ttgtcgaaaa
     3781 agctcatacc tgtttccttt gttgctgtgt tatttagaac cggcgctcca gcgaaatccc
     3841 cagccaaatg cccggtccat ttcgtcctgg cctttgaagt actggttaat tcgctcgact
     3901 ttaattgcgc cgttttcgtt tacgagtctg gcaatggcgc acaatgtgcg acggttctca
     3961 ccttcggtca ggcgggtctc aatgggcggt tgatccggca catgaatggt gactacacca
     4021 tcagtcttgt cccagctggg aacaccttca taaataaagg cgtaaatcag cacttcgcgg
     4081 atatgcttcc attcacgtcc attgatatgc agccactcgc cgtctgatac atcacccgtc
     4141 cggtcatcgc ccttgagctg aacatacggt tcatcgcgat aatcaccgaa tgcattaccg
     4201 agcgcctgaa tgaccgattt atacccgtcc tgaagctcaa caaaggcccc cagatccagg
     4261 tcgattcctt tggagccgaa catacctgca aagcctgatt tgctactgcc ccggtgccag
     4321 ttcaggttga tccggatttc accgaagtta tcccgtttgg tcaggctgat tgcaggcttc
     4381 tcttttgtca gcgatacctt gctcaggctg atttttgtct caactggcgg aggggttaca
     4441 accggaggag ttggggctgc gggagccggt tcgtcggcaa tatttacgcc gaaatgctct
     4501 gccagcggtt taagtccacc gttgaacccc tgcgcaacga agcggaattt ccagtcatta
     4561 ttacgacggt aaaattcccc cagaattaag gctgcttcct gacggccgct cagttcgaca
     4621 ctgccgctaa ccaggccggt agccccttgc tccacgtcga tggaaagatt acgaagaccg
     4681 gacaccgtct gcccaccgtc acaggtcacg gtgaacgcaa ttttctggac atcaggtttc
     4741 agccggttga gcgcgacagt aaaggttgag tactggcctt cactgaccag gctgacggta
     4801 ccatcatcgt tacgtggctg accataaaac accatgtccg catcaccctg caccttcccg
     4861 tcggcataca ggcgaaacgc cgaagcatcc acctggccgc ccgaggtgat ccggacccgc
     4921 aactcctggg ctggaacggg ggcattgccc cccggcgtca ggttcattag cgaataaccg
     4981 ctgaaacgat atcggagtgc atatcgtcaa ttgtgcggcc gcgacaggca tggccaaggg
     5041 cggtaaagtc ccagttgccg ttattacggc gcagcgaaga aataacgatc ccggtatgtg
     5101 agccctgctc agtcagcttg tagcgagcca gctctttgcc ggtttgatcg acaacgcggc
     5161 agaaagcgtt ttcgacgtca ttgaacgact gaccacggaa gctgtttacc gtaaacgcca
     5221 ggtattcaac gtttgcaggc aggcgggaga ggttgacctt aatcacttca tcgtcaccgt
     5281 caccctcacc ggtcaggtta tcgccactgt gcacaacagc gccgcaggtg gatttcagtt
     5341 tgcggaacca gatggtgtca attgttttac ctgtgctgtc cattaataca cagccagcat
     5401 cgagatcgat tgagtcgttg cctccaaaca atcctccaag aagacctttt ttcttaaccg
     5461 gatcccaacc gagaccgaaa tgaagctggc ttaatgcaga tgattctttg ctgagtgata
     5521 ccgtctggtt cttaactaat gaaaccatgt tttattcctt aaaatggtaa tgaccgagac
     5581 aatcaaaggt atatttagta actgacgaag aaaatcatat catgatgaag ttctctgaca
     5641 agggaaattt ttgtggtttt ctgtaaaaac
//
LOCUS       DAZKFZ010000260         5634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000260 DAZKFZ010000000
VERSION     DAZKFZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            22..564
                     /gene="fimA"
                     /locus_tag="ACHOQW_004371"
     CDS             22..564
                     /gene="fimA"
                     /locus_tag="ACHOQW_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9575323.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            784..1476
                     /gene="fimC"
                     /locus_tag="ACHOQW_004372"
     CDS             784..1476
                     /gene="fimC"
                     /locus_tag="ACHOQW_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9575324.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1507..4116
                     /gene="sfmD"
                     /locus_tag="ACHOQW_004373"
     CDS             1507..4116
                     /gene="sfmD"
                     /locus_tag="ACHOQW_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP9575325.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4129..5136
                     /gene="fimH"
                     /locus_tag="ACHOQW_004374"
     CDS             4129..5136
                     /gene="fimH"
                     /locus_tag="ACHOQW_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9575326.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5147..>5634
                     /gene="sfmF"
                     /locus_tag="ACHOQW_004375"
     CDS             5147..>5634
                     /gene="sfmF"
                     /locus_tag="ACHOQW_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP9575327.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KA"
BASE COUNT         1472 a         1219 c         1365 g         1578 t
ORIGIN      
        1 aatgagattg aaggaatata tatgaaatta agatttattt cgtctgcgct ggctgccgca
       61 ctattcgccg ctacgggtag ttatgctgcc gttgtagatg gcggtacaat tcactttgaa
      121 ggtgaactgg tgaatgctgc ctgttcagtg aatactgact cggcagacca ggttgtcaca
      181 ctcggtcaat atcgtaccga tattttcaat gctgttggta atacctctgc attaattcca
      241 ttcaccattc agttgaacga ctgcgatcct gttgttgccg ctaatgctgc cgttgcattt
      301 tctggtcagg ctgatgcaat caatgataat ttattggcca ttgcatccag taccaataca
      361 acaacagcaa cgggtgtcgg tattgaaata cttgataata catccgcaat tctcaaacct
      421 gatgggaata gcttctcaac caaccagaac ttgatccccg ggaccaacgt tcttcatttt
      481 tctgcacgtt ataaaggcac cggtacaagt gcatcagcag ggcaagcaaa tgctgacgcg
      541 acttttatta tgagatacga ataatcaaaa ccacgttgtt ttgaattata tatcacgtct
      601 tataacaaag taatgtaccg gttgtctgaa gcggtatggt ggcaatgtaa atcgaaatca
      661 tgttcacttt gtatcatgcc gctttattaa atgaaaaggg aatgatgtgt tgtaagaaac
      721 caaagcaatc atttctttat atttcttatt tttgccgtca ggaatacaca aggcgtatta
      781 actatgatga ctaaaataaa gttattgatg ctcattatat tttatttaat catttcggcc
      841 agcgtccatg ctgccggagg gatcgcatta ggtgccacgc gtattattta tcccgctgat
      901 gctaaacaga ctgcggtatg gattagaaat agccatacca atgagcgttt tctggtcaat
      961 tcgtggattg aaaacagcag cggtgtaaaa gaaaagtcat tcatcattac accgccacta
     1021 tttgttagtg aacccaaaag cgaaaatact ttgcgtatta tttacaccgg tccaccgctg
     1081 gcagcagatc gtgagtctct gttctggatg aatgttaaga cgattccttc ggtagataaa
     1141 aatgcattga acggcaggaa tgttttgcaa ctggcgattt tatcgcgcat gaagttattt
     1201 ctccgtccaa ttcaactaca agaattaccc gcagaagcgc cggatacact caagttttcg
     1261 cgatccggta actatatcaa tgttcataat ccatcacctt tttatgtcac cctggttaac
     1321 ttacaagtgg gcagccaaaa gttggggaat gctatggctg cacccagagt taattcacaa
     1381 attcccttac cctcaggagt gcagggaaag ctgaaatttc agaccgttaa tgattatggt
     1441 tcagtaaccc cggtcagaga agtgaactta aactaaccga atcatctgac aatatcagag
     1501 ctaattatga aaatacccac tactacggat attccgcaga ggtatacctg gtgtctggcc
     1561 ggaatttgtt attcatctct tgccatttta ccctcctttt taagctatgc ggaaagttat
     1621 ttcaacccgg catttttatt agagaatggc acattcgttg ctgatttatc gcgctttgag
     1681 agaggtaatc atcaacctgc gggcgtgtat cgggtggatc tctggcgtaa tgatgagttc
     1741 attggttcac aggatatcgt atttgaatcg acaacagaaa atacaggtga taaatcaggt
     1801 gggttaatgc cctgttttaa ccaggtactc cttgaacgaa ttggccttaa tagcagtgca
     1861 tttcccgagt tagcccagca gcaaaacaat aaatgcatca atttactgaa agctgtacct
     1921 gatgccacaa ttaactttga ttttgcagcg atgcgcctga acatcactat tcctcagata
     1981 gcgttgttga gtagcgctca cggttacatt ccgcctgaag agtgggatga aggtattcct
     2041 gctttactcc tgaattataa tttcaccggt aacagaggta atggtaacga tagctatttt
     2101 tttagtgagc tcagcgggat taatattggc ccgtggcgtt tacgcaacaa tggttcctgg
     2161 aactattttc gcggaaatgg atatcattca gaacagtgga ataatattgg cacctgggta
     2221 cagcgcgcca ttattccgct gaaaagtgaa ctggtaatgg gagacggcaa tacaggaagt
     2281 gatattttcg atggtgttgg atttcgtggt gtacggcttt attcttccga taatatgtat
     2341 cctgatagcc agcaagggtt tgccccaacg gtacgtggga ttgcccgtac ggcggcccag
     2401 ctaacgattc ggcaaaatgg ttttattatc tatcaaagct atgtttcccc cggcgctttt
     2461 gaaattacag atttgcaccc gacatcttca aatggcgatc tggacgtcac catcgacgaa
     2521 cgcgatggca atcagcagaa ttacactatt ccgtattcaa cagtgccaat tttacaacgc
     2581 gaagggcgtt tcaaatttga cctgacggcg ggcgattttc gtagcggtaa tagtcagcaa
     2641 tcatcgcctt tcttttttca gggcacggca ctcggcggtt taccacagga atttactgcc
     2701 tacggcggga cgcaattatc tgccaattac accgcctttt tattagggct ggggcgcaac
     2761 ctcgggaact ggggcgcagt gtcgctggat gtgacccatg cgcgcagtca gttagccgac
     2821 gacagtcgtc atgaggggga ttccattcgc ttcctctatg cgaaatcgat gaacaccttc
     2881 ggcaccaatt ttcagttaat gggttaccgc tattcgacac aaggttttta tacccttgat
     2941 gatgttgcgt atcgtcgaat ggaggggtac gaatatgatt acgattatga cggagagcat
     3001 cgggatgaac cgataatcgt gaattaccac aatttacgct ttagccgtaa agaccgtttg
     3061 cagttaaata tttcacaatc acttaatgac tttggctcgc tttatatctc tggtacccat
     3121 caaaaatact ggaatacttc ggattcagat acctggtatc aggtggggta taccagcagc
     3181 tgggttggca tcagttattc actctcattt tcgtggaatg aatctgtagg gatccccgat
     3241 aacgaacgta ttgtcggact taatgtttca gtgcctttca atgttctgac caaacgtcgc
     3301 tacacccggg aaaatgcgct cgaccgcgct tatgcctcct ttaacgccaa ccgtaacagc
     3361 aacgggcaaa atagctggct ggcaggtgta ggtgggacct tactggaagg ccacaacctg
     3421 agttatcacg taagccaggg tgatacctcg aataatgggt atacgggcag cgccacggca
     3481 aactggcagg ccgcttacgg tacgctgggg gtcgggtata actacgaccg cgatcaacat
     3541 gacgttaact ggcagctgtc tggcggtgtg gtcggacatg aaaatggcat aacgctgagc
     3601 cagcctttag gggataccaa tgttttgatt aaagcgcctg gcgcaggcgg tgtacgcatt
     3661 gaaaatcaaa ctggcatttt aaccgactgg cgcggctatg cggtgatgcc gtatgccacg
     3721 gtttatcggt ataaccgtat cgcgcttgat accaatacga tggggaattc catcgatgtt
     3781 gaaaaaaata ttagcagcgt tgtgccgacg caaggcgcgt tggttcgtgc caattttgat
     3841 acccgcatag gcgtgcgggc gctcattacc gttacccagg gcggaaaacc ggtgccgttt
     3901 ggatcactgg tacgggaaaa cagtaccgga ataaccagta tggtgggtga tgacgggcaa
     3961 gtttatttaa gtggtgcgcc attgtctggt gaattactgg ttcagtgggg agacggcgcg
     4021 aactcacgct gcattgcgca ctatgtattg ccgaagcaaa gcttacagca agccgtcact
     4081 gttatttcgg cagtttgcac acatcctggc tcataaagga aattatcaat gaagataatc
     4141 tgcagattat tattggcgat ggcatgtttg tgtctggcaa acatatcctg ggctactgtt
     4201 tgtgcaaata gtactggcgt agcagaagat gaacactatg atctctcaaa tgtctttaat
     4261 agcaccaata accagccagg gcagattgtt gttttaccgg aaaaatccgg ctgggtaggt
     4321 gtctcagcaa tttgtccacc cggcacgctg gtgaattata cataccgtag ttatgtcact
     4381 aactttattg ttcaggaaac tatcgataat tataaatata tgcaattaaa tgattatcta
     4441 ttaggtgcga tgagtctggt tgatagtgtg atggatattc aatttccccc gcaaaattat
     4501 attcgtatgg gaacagatcc taacgtttcg caaaaccttc cgttcggggt gatggattct
     4561 cgtttgatat ttcgtttaaa ggttattcgg ccctttatta acatggtgga gatccccaga
     4621 caggtgatgt ttaccgtgta tgtgacatca acgccttacg atccgttggt tacacctgtt
     4681 tataccatta gttttggtgg ccgggttgaa gtaccgcaaa actgcgaatt aaatgccggg
     4741 cagattgttg aatttgattt tggtgatatc ggcgcatcgt tatttagtgc ggcagggccg
     4801 ggtaatcggc ctgctggtgt catgccgcaa accaagagca ttgcggtcaa atgtacgaat
     4861 gttgctgcgc aggcttattt aacaatgcgt ctggaagcca gtgccgtttc tggtcaggcg
     4921 atggtgtcgg acaatcagga tttaggtttt attgtcgccg atcagaacga tacgccgatc
     4981 acgcctaacg atctcaatag cgttattcct ttccgtctgg atgcagctgc ggcagccaat
     5041 gtcacacttc gcgcctggcc tatcagtatt accggtcaaa aaccgaccga agggccgttt
     5101 agcgcgctgg ggtatttacg cgtcgattat caatgaggta cggagaatga gaagagtatt
     5161 ctttagctgt ttctgcgggt tactgtggag ttccagtgga tgggcagctg accctttagg
     5221 aacgattaat atcaatttgc acggtaacgt tgttgatttc tcctgtaccg taaacacagc
     5281 ggatattgat aagacggtag atttaggcag atggcctacg acacaattac tgaacgctgg
     5341 cgataccacg gcactcctcc cttttagcct gcggctggag ggatgtcctc cgggttcagt
     5401 tgcgatttta tttacgggaa cgccggcatc tgatgccaac ctgctggctc tggatgatcc
     5461 tgcaatggca caaaccgtcg ccatcgaatt acgtaatagc gatcgctccc ggctcgcact
     5521 gggggaggcg agcccgactg aggaagtaga tgcaaatggc aatgtcacac taaacttttt
     5581 tgccaattat cgagcgttag ccagcggtgt tcggccaggt gtggcgaaag cgga
//
LOCUS       DAZKFZ010000261         5393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000261 DAZKFZ010000000
VERSION     DAZKFZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..152
                     /locus_tag="ACHOQW_004376"
     CDS             <1..152
                     /locus_tag="ACHOQW_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HFP9575328.1"
                     /translation="LPVILKNERQINVFLRTEDIDLINVINEETLLQQPEERFAWLRS
                     KKDRF"
     gene            224..1582
                     /gene="mltD"
                     /locus_tag="ACHOQW_004377"
     CDS             224..1582
                     /gene="mltD"
                     /locus_tag="ACHOQW_004377"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP9575329.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(1630..2400)
                     /locus_tag="ACHOQW_004378"
     CDS             complement(1630..2400)
                     /locus_tag="ACHOQW_004378"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9575330.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(2478..3278)
                     /gene="yafD"
                     /locus_tag="ACHOQW_004379"
     CDS             complement(2478..3278)
                     /gene="yafD"
                     /locus_tag="ACHOQW_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9575331.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3519..4433
                     /gene="yafC"
                     /locus_tag="ACHOQW_004380"
     CDS             3519..4433
                     /gene="yafC"
                     /locus_tag="ACHOQW_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP9575332.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4430..5233)
                     /gene="dkgB"
                     /locus_tag="ACHOQW_004381"
     CDS             complement(4430..5233)
                     /gene="dkgB"
                     /locus_tag="ACHOQW_004381"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP9575333.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1427 a         1283 c         1335 g         1348 t
ORIGIN      
        1 cactacccgt aattctgaaa aatgagcggc aaattaatgt ttttttaaga acggaagata
       61 ttgatttaat taatgtaatt aatgaagaaa cattattgca acaacctgaa gagcgttttg
      121 catggttaag gtcaaagaaa gataggttct gataaaactt tcttgtcatc ggctccgttc
      181 gccgttatga tcggtcgtct tttaagcaac tattgacgca cacatgaagg caaaagcgat
      241 attactcgcc tctgtcctgc tcgtgggttg ccagagtacc ggcaacgttc aacagcacgc
      301 acagagcctt tctgcagctg gtcaagggga agcagcaaag tttacaagtc aggcacgatg
      361 gatggacgat gggacgtcta tcgcgccaga tggtgacttg tgggctttca ttggcgacga
      421 gctaaagatg ggaattccgg aaaatgaccg gattcgcgaa cagaaacaga aatatttacg
      481 caataagagc tatctccacg atgtaacttt acgggcagag ccgtatatgt actggatagc
      541 cgggcaagtt aaaaaacgta acatgcctat ggaactggta ctactaccca tagtggagag
      601 cgcttttgat cctcacgcaa cgtctggcgc caatgccgca ggcatctggc agatcattcc
      661 gagcacgggg cgcaattatg gtttgaaaca gacccgcaat tatgacgcgc gtcgcgatgt
      721 tgttgcttca acaactgccg cgctgaatat gatgcagcgt ctgaacaaaa tgtttgatgg
      781 cgactggctt ctgaccgtag cggcttataa cagcggcgaa ggtcgggtca tgaaggcaat
      841 taaaacgaac aaagcgcgtg ggaaatccac ggacttctgg tcgttaccgt tgccgcagga
      901 aacgaagcag tacgtgccta aaatgctggc attgagtgat attctcaaaa acagcaagcg
      961 ttatggcgta cgtctgccaa cgaccgatga aagccgtgct ctggcgcgtg tgcacctgag
     1021 tagcccggtt gaaatggcga aggttgcaga tatggcgggg atttccgtca gcaagctgaa
     1081 gacattcaac gctggcgtga aaggctccac gctgggcgca agtggtccgc agtacgtgat
     1141 ggtgccaaag aagcatgcag atcaactgcg tgaatctctg gcttcaggcg aaattgctgc
     1201 tgtacagtcg acgctggttg ccgacaatac gccgcttaac agccgtgttt acaccgtacg
     1261 ctctggcgac acgctttcaa gtatcgcttc acgtctcggc gtaagcacca aagatttgca
     1321 gcagtggaac aaactgcgcg gctctaagct gaaaccaggc caaagtctga cgattggcgc
     1381 aggtagtagc gcacagcggt tggcaaacaa cagcgatagc attacgtatc gtgtgcgcaa
     1441 aggcgattcg ctttcaagca ttgctaaacg ccacggcgtg aacatcaaag atgtgatgcg
     1501 ctggaacagc gatactgcga atctgcaacc aggcgataag ctgacgttgt ttgtgaaaaa
     1561 caacagcatg ccagactcct gacaaaccag ataatagaaa ggcaccgatt cccccggtgc
     1621 cttttttatt tatgccgctt tatgtgcatc taccatgatg atatcactgg tgaaagagcc
     1681 atcattctgc aaggcaaaat acgttctcac ctctgtcgat gcgctctgtt ggtaaatgcg
     1741 aatagcgtct actaacgctt ctggcgtacg cattctcgcg acccatgaag aaaattccag
     1801 cggtaactta tctgtaatta aattatcaac tatcagattg gcttcattga ttaacctcaa
     1861 ccactcaccg ctggcgtagt ttcgtacgtg agaggtatcg cataatgctt ctaccgtctg
     1921 taaccagatg tcgcgcactg ggtggcccgg agacattacg tccatcacaa tcagcctacc
     1981 gccaggtttc aatatccggt tcacttcacg cagtgctgca ccaacatcat gccagtgatg
     2041 ggcagaataa cggctgataa caatatcaaa tgcgttatcg gcaaatggca gactttcggc
     2101 atacccctgg cgggtggtga tatttttcag ttgccggact tcggcagctt gtgccacgac
     2161 atccagcatt tgggcagata agtcatacgc caccaccgtg ctcacatttt gcgcagcgac
     2221 aaagctggca tgccctgctc cgcagcccat atcaagtaca cttgcatcag gataatcagc
     2281 cagacacacc gccaggagct gtaaatctcg tccggatgca tgtacggtac tggtcagata
     2341 ttcgctggct tgagagctaa actgcttttc tacgtggtca tggtgggatt gtgttgtcat
     2401 tgcactgtcc ttttgttgtt tgagtataaa agggcagctc caaacgttgg tctgccctgt
     2461 ggcagacctg acatacctta tttatcaggc ttgccgggac tgaattcaac gagtagcgga
     2521 ttgtgatcgg acgcgcgcgt aaccagtaca gaagcttcgc tgacgttcag accacggtag
     2581 aaaacaaaat cgagcgggcg accaaacgcc cgacggcgct gatcatcggt aaaacgcacc
     2641 tgacgcagcg acatttcccg cgcaaaacga tataacgcgt tcatccttct acggctccaa
     2701 gcattgaaat ctcccgccat aatgaccggg ccgctgtggt gagctatctg atcaccaata
     2761 ggaagtaact gcttactata aacatccacg cccagactga agttgacggc gtgtatatta
     2821 accaccatta acaggcgggt gtcaggtaat ggatagaccg tcaccagtgc cgacttcgcc
     2881 agacgcaaaa tgggttctcg ttcacgtaac gggcagcaat acactggatg tgccgccgaa
     2941 agggtcatta cgccagaagg atgttgtggc agcacgaaag cgggtacctg atcggcggca
     3001 agatagttag cagtcgcaaa ctgtaccaac tctggcgttg tttgcgcttc ctgcaataac
     3061 accagatgtg cgtctttgcc gtaattcttt aataccgaca accattcagc gcgttgctgt
     3121 ttgtatatgt tccacaccag gatccgaata cgctcttcgg tacttaacgg ttctccgggt
     3181 ggtaatgcct ggccgatgct cgcaaaagac cccggcggta agatcctctc cgcaggttgt
     3241 ccggcaacat agcgcatggc ataggtgttt tttcgcactt ttgactttga aacctctatt
     3301 acgtcaaccc ttccgcactc gggagggttc tactgttata gggattttag ctcactcttt
     3361 caacgagcaa ttgtatattg ttatgtaagc aagtgcttac accggtcaga attttgctca
     3421 ttattgagaa cattaaatcg gatgacatat cacgcatcct gatggtttaa tgacaaataa
     3481 attcaaaata tctcatctat tttgcaacag gagcaaaaat gaaagccacg tcggaagaac
     3541 tcgccatttt tgtttcggtc gtcgaaagcg gcagctttag ccgggcagcg gaacaattag
     3601 ggcaagcaaa ctcagcggta agccgggcgg tgaaaaagct ggagatgaaa cttggcgtta
     3661 gcctgcttaa tcggaccacg cggcaactta gcctgacgga agaaggcgag cgttattttc
     3721 gtcgcgtaca gtcaattttg caggagatgg cagcggcaga atcagaaatt atggagacgc
     3781 gtaatacacc gcgtggactg ttgcgcatcg atgccgcaac tccagtggtg ctgcactttc
     3841 tgatgccgtt gattaagcct tttcgtgaac gctatccgga agtcacttta tcgctagtct
     3901 cctccgaaac gattattaat ttgatcgaaa gaaaagtgga tgtcgcgata cgcgccggta
     3961 cgttaacgga ttccagctta cgtgccaggc cgttatttaa cagttatcga aaaattatcg
     4021 cctcccccga ttatatttcc cgctacggga agccagaaac gatcgacgat ttaaagcaac
     4081 atgtttgcct gggattcact gaacccgctt ccctcaatac ctggccggta gcctgtagcg
     4141 atggacaatt acatgaggtg aagtacggtt tgtcatccaa tagtggggaa acactgaaac
     4201 agctttgcct gagtggcaac gggattgcgt gtttgtccga ctatatgatc gacagagaaa
     4261 tcgctcgcgg agaattggtg gagttaatgg cagataaagt gttgccagtg gaaatgccat
     4321 tcagtgccgt ctattacagc gaccgtgcgg taagtacgcg catccgggct tttatcgatt
     4381 tccttagcga gcatgtaaaa acagctcccg gaggagctgt cagagaggct taatcccatt
     4441 caggagccag accttccggg ctaaccaggc ggtcgttgca atccagtgcg gcgatcgctt
     4501 ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag attactctcc aggttttcac
     4561 gtttagtaga agaaggaatt actgagtaac cttcccccat agcccacgcc agaatcactt
     4621 gtgccggagt cgcattgtgt ttagctgcga tacgagcaat aacctcatct ttcagggcct
     4681 taccatacgc cagcgtcata taggaagtaa tatggatgcc gtgctgttta gcccaggcaa
     4741 ccactttacg gttttgcaga taaggagaga gttcaatctg gttagtagcg atgttttcag
     4801 cgccaacagc agcaatcgcc ttttccatca atgggatcgt gaagttggaa ataccgatct
     4861 cacgcgtcaa cccttctttt ttggcttcca gcagcgcctg cataaactct tcaacagaga
     4921 cttcatcgtt tggtgacggc cagtggatta gcgtcagatc aacataatcg gtacgcaatt
     4981 tttgcaggct ctcttttaga ctcgggatca atttgtcttt gctgagattt tcaatccaga
     5041 ttttagtggt gatgtagagt tcatgacgtg gcacgccact ttctgcaatc gccagaccta
     5101 ctgcggcttc gttatcatag atttgtgcag tatcaattgc gcgataacca agttcaagcg
     5161 ccgttttcac agatgaaata acaacgtcgt ctttcagacg gaaagtacct aaaccaaatg
     5221 cagggatagc cataatatgc ctcttttaat gctattttcg ttaactgagg tgattatggg
     5281 ctttagaatg ttgcggaaaa agggcgtaaa acgcagagga tttttgcgat tttagcaata
     5341 atagaaatgt agacgaaaaa aaacctcgag cgataactcg aggcttctta ata
//
LOCUS       DAZKFZ010000262         5254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000262 DAZKFZ010000000
VERSION     DAZKFZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            138..4040
                     /gene="espP"
                     /locus_tag="ACHOQW_004382"
     CDS             138..4040
                     /gene="espP"
                     /locus_tag="ACHOQW_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFP9575334.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4296..4777)
                     /locus_tag="ACHOQW_004383"
                     /pseudo
     CDS             complement(4296..4777)
                     /locus_tag="ACHOQW_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1582 a         1145 c         1184 g         1343 t
ORIGIN      
        1 tgtttgttat ccgtatgata acaaacacat aaaggagtaa acatttatct ggacgatgat
       61 tacaatgttc acgactgtca gagacacacc tctgatacat tagaaaaacc aatgtttccc
      121 ttaaaaatgg agcttatatg aataaaatat actctcttaa atacagccat attacaggag
      181 ggttaatcgc tgtttctgaa ttatccggca gagtatcatc aagagcaact ggtaagaaaa
      241 aacacaaacg catacttgca ttatgttttt taggcttatt acaatcctca tattcttttg
      301 cgtcacagat ggatatttca aatttctaca tccgtgacta tatggatttt gcacagaaca
      361 agggcatatt tcaggctggc gcaacaaata ttgaaatagt gaagaaagat ggctccaccc
      421 tgaaactacc ggaagtacca tttcctgact tctcaccggt tgcaaacaaa gggtcaacca
      481 catctattgg tggtgcatac agtatcacag ccacacacaa tacgaaaaac caccactcag
      541 ttgcgacgca aaactggggg aacagcacgt acaaacaaac tgactggaat acttcacatc
      601 ctgattttgc agtatcccga cttgacaagt ttgttgttga gacccgaggt gcgactgaag
      661 gcgcagatat ttcgttatca aaacagcagg cacttgaacg ttacggggtt aattataaag
      721 gagaaaagaa acttatcgca ttcagagccg gctctggtgt ggtatccgtt aaaaaaaatg
      781 gacgcataac tccatttaat gaggtttctt ataagccaga aatgttaaat ggctctttcg
      841 ttcacattga tgactggagt ggatggctga tattaaccaa caaccagttt gatgagttta
      901 ataacattgc ctctcagggt gacagcggtt cagcactgtt cgtctatgat aaccaaaaga
      961 aaaagtgggt tgtcgctgga actgtctggg ggatttataa ttacgccaat ggcaaaaacc
     1021 acgcagcata cagtaaatgg aaccagacaa ccattgacaa cctgaagaac aagtattctt
     1081 acaacgtgga tatgtcaggg gctcaggttg caaccattga aaatggaaaa ctgacaggca
     1141 ctggctcaga caccaccgat ataaaaaata aggacttaat atttactggc ggtggagata
     1201 tcctcctgaa atcctctttt gataatggtg ctggcggtct tgtctttaat gataaaaaga
     1261 cctatcgagt aaacggggat gatttcacct ttaaaggtgc cggtgttgat acaagaaacg
     1321 gcagcaccgt tgagtggaat atccggtatg ataataaaga caaccttcac aaaattggtg
     1381 atggcacatt agatgtccga aaaacccaga acaccaacct gaaaacaggt gagggtcttg
     1441 tcattcttgg agctgaaaaa acattcaata atatctacat aaccagtggt gatggaactg
     1501 tccgactgaa tgcagaaaat gcactgtctg gcggtgaata caacggtatt ttctttgcga
     1561 aaaatggcgg aactcttgac ctgaacggat ataatcagtc tttcaataaa attgctgcaa
     1621 ctgattcagg tgctgtaata accaatacgt caaccaaaaa atccatttta tccctgaata
     1681 atactgctga ctatatctat cacggcaaca taaacgggaa tctggacgta cttcagcatc
     1741 atgagacgaa aaaagagaac cgtcgtctta ttcttgatgg gggcgtggac acaacaaatg
     1801 atataagcct gcgtaataca caactgtcca tgcagggaca tgccactgaa catgccattt
     1861 atcgggatgg agctttctct tgttcactac cagctcctat gcgctttttg tgtggcagtg
     1921 attatgttgc aggaatgcaa aatacagaag ctgatgctgt aaaacaaaac ggaaatgcct
     1981 ataaaaccaa caatgctgtc tctgatttat cgcagccaga ctgggaaacc ggaacattca
     2041 gatttggaac gctacatctt gaaaattccg atttttctgt tggtcgtaat gcaaatgtaa
     2101 tcggggacat tcaggccagt aaatcaaaca ttactattgg tgacactaca gcatatattg
     2161 atttgcatgc tggtaaaaat attaccggtg atggttttgg cttccgccag aatattgtgc
     2221 gtggaaactc acaaggagaa acgctgttta caggagggat cacagcagaa gacagcacta
     2281 tcgttattaa agataaagca aaagcattat tttcaaatta tgtatacctg ctgaacacaa
     2341 aagcaaccat agagaacggt gctgatgtga caactcaaag tggtatgttc tccacgagcg
     2401 atatcagcat ctctggtaat ctgtccatga caggcaatcc cgacaaagac aataaattcg
     2461 agccctcaat atatctgaat gatgcttctt atctactgac tgacgactcc gccagactcg
     2521 ttgccaaaaa taaagcatct gtggtgggag atatacactc cactaaaagt gcatccatca
     2581 tgtttggtca tgatgaaagc gacctctcgc agttgtctga cagaacctca aaagggcttg
     2641 cacttggtct tttaggtggc tttgatgtct catatcgcgg ttcagtcaat gccccgtcag
     2701 catctgccac tatgaacaac acctggtggc aactaaccgg agattctgcg ctgaaaacac
     2761 tgaaaagtac aaacagcatg gtctatttca ctgacagcgc aaacaataag aaattccata
     2821 cgctgacggt cgatgagctg gcaaccagca acagcgccta tgcgatgcgt acaaaccttt
     2881 ctgaatcaga caaactggag gtcaaaaaac acttgtctgg tgagaacaat attttactcg
     2941 ttgatttcct tcagaaacca acgcctgaaa aacaactgaa tattgaactg gtaagcgcgc
     3001 caaaagacac caatgaaaat gtctttaaag ccagtaaaca aaccattggt ttcagtgatg
     3061 taacgccggt cattacaacc agggaaaccg atgacaaaat aacatggtca ctgacaggct
     3121 ataacacggt agcaaacaag gaagcaaccc ggaatgccgc cgccctgttc tctgttgact
     3181 ataaagcgtt tctgaacgag gtcaacaacc tgaacaaacg tatgggtgac ctgcgtgata
     3241 tcaacggcga agccggtgca tgggcacgca tcatgagcgg taccggctct gccagtggtg
     3301 gtttcagtga caactacacg cacgttcagg tcggggtcga caaaaaacac gagctggacg
     3361 gactggattt gtttaccggt ttcactgtca cacacactga cagcagtgcc tccgccgatg
     3421 ttttcagtgg taaaacgaag tctgtggggg ctggcctgta tgcttccgcc atgtttgatt
     3481 ccggtgccta tatcgacctg attggcaagt atgttcacca tgataatgag tacactgcaa
     3541 cctttgccgg actcggaacc cgtgattaca gcacgcattc atggtatgcc ggtgcagaag
     3601 cgggctaccg ctatcatgtc actgaggatg cctggattga gccacaggct gagctggttt
     3661 acggttctgt atccggtaaa cagtttgcat ggaaggacca gggaatgcat ctgtccatga
     3721 aggacaagga ctacaatccg ctgattggcc gaacgggtgt ggatgtgggt aaatccttct
     3781 ctggtaagga ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg
     3841 ctaacggcga aaccgtattg cgggatgcat ctggtgaaaa acgcatcaaa ggtgaaaagg
     3901 acagccgtat gctgatgtcc gttggcctga atgcagaaat cagggataac gtccgctttg
     3961 gactggagtt tgagaaatcc gcctttggta agtacaacgt tgataatgct gtcaacgcta
     4021 atttccgtta ctcgttctga tactccgtat tcaaaagggc ctgcgggccc tttacgctca
     4081 gtgcctgccg ccctgatatg tctgagagac atttccggga tgtcagtcga ctggtttact
     4141 taactgcgat gactaaagct ggcagtaatg ccggcttttt tacggccaca tacgtcttcc
     4201 tctcatccgg aactgtgata caaatcctgc atacaggatt gaccgccggt tcctgcaaat
     4261 ggcacatttt cagtaagata gcgaaggtta catttgtcac tggttaacat accgatggta
     4321 aaaggggcaa gcagcgccag caattcatca gcccgggggc caaaagtgta ggccagaacg
     4381 ccaccagttt tcgtgttgta cgcgtaccag agcctgtgct gtcacgcttt actgccgata
     4441 aaactccatt gctcatcaag ttcgcagatg agtgccatat cagcatgagc gaccggggat
     4501 gacgttattc ttcgtggcga gcgtttttta aagtccgaat gacagtatta atgccgattt
     4561 tcagcgtacg ggcagtatcg cgaactccgg caccgttgaa aggcatttca gtgatttggt
     4621 cttgaactcc cggtttacgg gcctcgtaac ggtaagtgag ctgaaacact cgatgacagt
     4681 cacggcagcg aaatcgatca tgtccttttg ggttcttccc gtgacggtaa acctgaacgg
     4741 actggcaacg ggggcaatga aggttaactg tgaccattaa agaaccttaa aagtgactat
     4801 tataccccta attcaactaa ttggaggcat taccatgcat tagtgcaagc gccttgtgga
     4861 ttcttttgct aatggcggaa acatattgta caatattgca tttatccctc aataaataaa
     4921 tgagttatca gattgcaaaa atctgttatc aaactaattc aaataaaaag agattgatac
     4981 gaccaacgta cacaaaaatt ttcgatattc gaactaaata acagctccta ataccgctac
     5041 actttagcta tccagtctgc gcctcagaaa tggacgatgc cgttgaagga ctggtgtatg
     5101 gcaatgagcc gctttattat cgaattcggt gaccgcttgg acggtcactt cggagaaaag
     5161 gcatttacac agcatcctaa acaggttcag cataggtgat ataccgcctt ttcattttct
     5221 tctccattat ttataaggga ggtgtattat tatg
//
LOCUS       DAZKFZ010000263         5214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000263 DAZKFZ010000000
VERSION     DAZKFZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.264"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..379)
                     /locus_tag="ACHOQW_004384"
     CDS             complement(<1..379)
                     /locus_tag="ACHOQW_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575335.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
     gene            complement(436..831)
                     /locus_tag="ACHOQW_004385"
     CDS             complement(436..831)
                     /locus_tag="ACHOQW_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9575336.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(847..1616)
                     /locus_tag="ACHOQW_004386"
                     /pseudo
     CDS             complement(847..1616)
                     /locus_tag="ACHOQW_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1642..2382)
                     /locus_tag="ACHOQW_004387"
     CDS             complement(1642..2382)
                     /locus_tag="ACHOQW_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9575337.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(2389..3351)
                     /locus_tag="ACHOQW_004388"
     CDS             complement(2389..3351)
                     /locus_tag="ACHOQW_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575338.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3374..3799)
                     /locus_tag="ACHOQW_004389"
     CDS             complement(3374..3799)
                     /locus_tag="ACHOQW_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9575339.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(3796..4098)
                     /locus_tag="ACHOQW_004390"
     CDS             complement(3796..4098)
                     /locus_tag="ACHOQW_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9575340.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4196..4567
                     /locus_tag="ACHOQW_004391"
     CDS             4196..4567
                     /locus_tag="ACHOQW_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575341.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            4588..4779
                     /locus_tag="ACHOQW_004392"
     CDS             4588..4779
                     /locus_tag="ACHOQW_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFP9575342.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            4781..5059
                     /locus_tag="ACHOQW_004393"
     CDS             4781..5059
                     /locus_tag="ACHOQW_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9575343.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT         1255 a         1450 c         1149 g         1360 t
ORIGIN      
        1 catccagagt taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg
       61 acgaaccctt tttttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg
      121 cagcatatag cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg
      181 agaatgtctt tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct
      241 gagcaacaag ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg
      301 tctgttttac tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct
      361 cttttgtaaa agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc
      421 tgatagctaa ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac
      481 acgcagcata acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta
      541 gcggcgtatt tctccgtctg gtaactttcg aatcaatgtc tggtcggttg ctttctccgc
      601 tgccttacgc catacgcgat acacctgttc tgatgtaaaa acaccgtatt taccgggcat
      661 gtataaatcg ccacaagcca gtacatccac aaggcaacgt ctgactgaat gccagcctgc
      721 tcccgtcgct ctctccagtt gtgatatcgt catgcgttca tttttgcgta ccagcccgat
      781 aattcgggcc ttcagttctt cacgctgttc gtgtgtaaaa ggtttcgcca taagcgcctc
      841 cggctatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
      901 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
      961 tttcgccctg cgcagcgcca ggtttgccct gcgcagggac ggaaaaatca gctcatctgc
     1021 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
     1081 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
     1141 cttaccatca tttttcgtta ccggcgcggc tttcggatct gctggtaaat taccccccga
     1201 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
     1261 caccgccagc gtggaagcaa ccttgcgagt actgacgccg aacttacccg ccagttcctc
     1321 acacgtttta gcaccatcct gaccgataaa ctcaaccatc atgtctgcgg taacttttgg
     1381 agcgacctct tcggttacca catccggcgc ttcaggttgt agtgcctgcc cttcggttac
     1441 cccggcttca ccttcgacag ccagaaacca ggtgtgaccc gttttatcaa caacgccatt
     1501 tttttgagtt cccacagttc gttaagaact tcttcacggc tgatatcaag ccgcgccgcc
     1561 agttcaacag aattggcttt tcccatcgct ttcagtgcat gcaatacagt ttccatcgaa
     1621 aatttacctc gtcaaaaatt ctcacatacc ctgacgtcca acgtttgacc gccagctctc
     1681 ccagttaaaa ttcacccaac gaccaccatt catggtcata cggtccatca cccgatctcc
     1741 gaggagtgtg ctcatcgctg cgtgattcag gttcgtcagc attccgacac tacgcatcga
     1801 agccgttctg cggtcgacta tctggttcag cgtgacctgc tcgttgcgcg tatcccgctg
     1861 catgccaatt tcatcaagga ccagaaggtc aactccacaa agctcctgta aaaatttttc
     1921 cccggactgg ccgttgtcgt agccgtcatg caacacgctc atgacatcgg acacggtgac
     1981 gataatcacg cttctcccct tcgccatcag ccgattgcca atcgctgctg ccaggtgatt
     2041 tttaccggta ccaggtttac cgctgaacac gaagtttgta catccggtca tcaattcatc
     2101 ggcaatggat ttcgcctggc tcagagcatg gcgctgaccg tcgttctgca cccggtagtt
     2161 cccgaatgag cacttcctgt gaagcggctg gatgcccgca cggttcagga ttttttcaac
     2221 ccgcacctga tgattcaggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg
     2281 ccattcccgc cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct
     2341 gcgaatacgt tcaagaaccc caactgccgc aatgtttttc atgacacgtc accccctgaa
     2401 tcccggcggt atttcagtgt ccggttcaga aatgtgattc acgcaacgct gcgcgggcga
     2461 acgccccagg cggataacca gttcatccca tttttcccgg agttttgccg gactcatgat
     2521 gttttttacc cagaacgaat cccgctggag acgcccaaac atttcacaaa tttgtctgtg
     2581 agttctgcca tccagcatcc gcattgtccg cacgtcattg gcccatgcag tccagttggg
     2641 ttctttcggt ctcgtgatct cgccatcatc gctggccgcc tgctcgtaaa gactcacgat
     2701 tcgtccccag atccactgtg cgcacaccaa atcttcctga cttccccact ggcgtttttt
     2761 cgcactgaac acaaccgcgt cagggtgtcg ggttaaaaaa ttctgttcag ccgtctgcgg
     2821 gtccggttgc gaagcgtccg gacaagaaga tcttttatct gacggatcag gttttaatac
     2881 tgacggatcg gggtcaatca tcgcccccct aatcggcagt ttgttatcaa cagttgatcc
     2941 atcaaaattt gacgggtcaa ccgttgaggg ggcaatattt gacgggtcaa ctgttaacgg
     3001 gtcatttttt gccgggctaa tttttctttt cggtttatat gactcacgcg ccgccgcagc
     3061 agctgcttcg agtttttcca cattaagccg atagatattg cttacattac gcccaccgac
     3121 cttacgctct tccttcgtca gccagccctc tttcgccagt tctgcaatag ccgatttcac
     3181 tgtggattca cttcttgcac cgatctgacg ccggatagtt tcaatggcag gccatgacac
     3241 gccctcgtca ttgctgtagt ctgcaagacg ggccataacc gccaccctgg ataagatcat
     3301 gccggtgaag gcgcaccctt cccagacaag accatgaagc ttgctgctca taaaaccccc
     3361 gaacaccgtg cttttagtgc atcaccacag cattccctgc cgggccgccg cgattcatct
     3421 ggtcatacaa aacaaccgct gacgcaacaa aatcatcgac atccttcacc agccgatccc
     3481 tccgttcgac gatctcacgg taatattcag aactgtggct gcgcatacgg gccaccagca
     3541 aaggcggcat cgccttttcg atcgccggta acagagcctg cattttttca acagcatcag
     3601 gggtgtcttt atccagccaa cggaaaattt tctgggtatt acgggccagg gcttccggat
     3661 ggctgtcgtc atacagttcc gggaacgtca ttcccagctc gaaatacgct ttggtaattt
     3721 tcgcagccgg tactttttcg ccgtccggat gcgcccagac attcatcgcc atgcggatgt
     3781 gttcatgctt gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc
     3841 agcatgaact acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag
     3901 ctcccaatca tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat
     3961 cataagggcg cgggttggag atatagctgt tcgtccagac gccatttgcg ataagtaaga
     4021 tggcgataca ccaagtctgg ccgcgaattt cttagcatca ccaaccctta atgatttaat
     4081 aaactctttt aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa
     4141 taaaccatta agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca
     4201 aaaaaaagaa attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc
     4261 caaagagaag agctacctat ctcaactaat gagtgggaga gcctcgtttg gagaaaaggc
     4321 tgccagaaga atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc
     4381 agaacaaccg gggggttctc caccacatgc agggttaacg tctaatcaac tggaattatt
     4441 gcagattttt tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca
     4501 gaaaaaagaa tcaatggaag atctcatagc gagatggatt gcggcgcaaa aatgccgccg
     4561 cgcctgagtt ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa
     4621 tttgaaacca ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg
     4681 agccttgcag atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa
     4741 cgctttgcta agatgcgcaa ggaacggagc aagcagtaaa atgttacccg tgttatggct
     4801 tgaaagcgca gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga
     4861 tgcggctgaa cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc
     4921 gtatttatac ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa
     4981 ctatataatt ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc
     5041 aagacgccag tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta
     5101 tcaaaagcgt tgtgtttgtt atgtcccgcg gtttagtttt tacttgactt aagtttaatg
     5161 tttattaaac taaaaatacc aacccacccc gccccacaga acgcagggaa atac
//
LOCUS       DAZKFZ010000264         5166 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000264 DAZKFZ010000000
VERSION     DAZKFZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.265"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            27..2057
                     /gene="malS"
                     /locus_tag="ACHOQW_004394"
     CDS             27..2057
                     /gene="malS"
                     /locus_tag="ACHOQW_004394"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9575344.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            2235..3488
                     /gene="avtA"
                     /locus_tag="ACHOQW_004395"
     CDS             2235..3488
                     /gene="avtA"
                     /locus_tag="ACHOQW_004395"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP9575345.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(3640..4113)
                     /gene="ysaA"
                     /locus_tag="ACHOQW_004396"
     CDS             complement(3640..4113)
                     /gene="ysaA"
                     /locus_tag="ACHOQW_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9575346.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(4215..4355)
                     /locus_tag="ACHOQW_004397"
                     /pseudo
     CDS             complement(4215..4355)
                     /locus_tag="ACHOQW_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            complement(4357..>5166)
                     /locus_tag="ACHOQW_004398"
     CDS             complement(4357..>5166)
                     /locus_tag="ACHOQW_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575347.1"
                     /translation="ELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRLTI
                     QEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLSVP
                     QQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAGTT
                     LAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVREAE
                     KLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
BASE COUNT         1279 a         1396 c         1309 g         1182 t
ORIGIN      
        1 atcgcagcaa taaggactca tccaccatga aactcgccgc ctgttttctg acactccttc
       61 ctggcttcgc cgttgccgcc agctggactt ctccggggtt ccctgccttt agcgaacagg
      121 gaacgggaac atttgtcagc cacgcgcagt tgcccaaagg tacgcgtcca ctcacgctaa
      181 attttgacca gcagtgctgg cagcctgcag atgcgataaa actcaatcag atgctttccc
      241 tgcaaccttg tagcaacacg ccgcctcaat ggcgattgtt cagggacggc aaatatacgc
      301 tgcaaataga cacccgctcc ggtacgccaa cattgatgat ttccatccag aacgccgccg
      361 aaccggtagc aaacctggtc cgtgaatgcc cgaaatggga tggattaccg ctcacgctgg
      421 atgtcagcgc cactttcccg gaaggagccg ccgtacggga ttattacagc cagcaaattg
      481 cgatagtgaa gaacggtcaa ataacgttac aacccgctgc taccagcaac ggtttactcc
      541 tgctggaacg ggcagaaact gacgcctctg cccctttcga ctggcataac gccacggttt
      601 actttgtgct gacagatcgt ttcgaaaacg gcgatcccag taatgaccag agttacggac
      661 gtcataaaga cggtatggcg gaaattggca cttttcacgg cggcgattta cgcggcctga
      721 ccaacaaact ggattacctc cagcagttgg gcgttaatgc tttatggata agcgccccat
      781 ttgagcaaat tcacggctgg gtcggcggcg gtacaaaagg cgatttcccg cattatgcct
      841 accacggtta ttacacacag gactggacga atcttgatgc caatatgggc aacgaagccg
      901 atctacggac gctggttgat agcgcacatc agcgcggtat tcgtattctc tttgatgtcg
      961 tgatgaacca caccggctat gccacgctgg cggatatgca ggagtatcag tttggcgcgt
     1021 tatatctttc tggtgacgaa gtgaaaaaaa cgctgggtga acgctggagc gactggaaac
     1081 ctgccgccgg gcaaacctgg catagcttta acgattacat taatttcagc gacaaaacag
     1141 gctgggataa atggtgggga aaaaactgga tccgtaccga tatcggcgat tacgacaatc
     1201 ctggattcga cgatctcacc atgtcgctag cctttttgcc ggatatcaaa accgaatcaa
     1261 ctaccgcttc tggtctgccg gtgttctata aaaacaaaac ggatacccac gctaaagcca
     1321 tcgacggctt tacccctcgc gattacttaa cccactggtt aagtcagtgg gtccgcgact
     1381 atgggattga tggttttcgg gtcgataccg ccaaacatgt tgagttgccc gcttggcagc
     1441 aactgaaaac cgaagccagc gccgcgcttc gcgaatggaa aaaagctaac cccgacaaag
     1501 cattagatga caaacctttc tggatgaccg gtgaagcctg gggccacggc gtgatgcaaa
     1561 gtgactacta tcgccacggc ttcgatgcga tgatcaattt cgattatcag gagcaggcgg
     1621 cgaaagctgt cgattgtatt gcgcagatgg atacgacctg gcagcaaatg gcggagaaat
     1681 tgcagggttt caacgtgttg agctacctct cgtcgcatga tacccgtctg ttccgtgaag
     1741 ggggcgacaa agcagcagag ttattactat tagcgccagg cgcggtacaa atcttttatg
     1801 gcgatgaatc ctcgcgtccg ttcggtccta caggttctga tccgctgcaa ggtacacgtt
     1861 cggatatgaa ctggcaggat gttagcggta aatctgccgc caacgtcgcg cactggcaga
     1921 aaatcagcca gttccgcgcc cgccatcccg caattggcgc gggcaaacaa acgacacttt
     1981 cgctgaagca gggctacggc tttgttcgtg agcatggcga cgataaagtg ctggtcatct
     2041 gggctgggca acagtgactt ttccggcttc ccgttcgtca gtacctcggg aagccgccaa
     2101 ccaggataaa atgtcagccc taatcagcgt tgcaggataa agcaccgctc actcttcaac
     2161 agaccgattt gcaccccagc aaatgtagcg ttattgttac cttccttgct acagagttcg
     2221 acagatatcc cgctatgaca ttctcccttt ttggtgacaa atttacccgc cactccggca
     2281 ttacgctgct gatggaagat ctgaacgacg gtttacgcac gcctggcgcg attatgctcg
     2341 gcggcggtaa tccggcgcag atcccggaaa tgcaggacta cttccagacg ctactgaccg
     2401 acatgctgga aagtggcaaa gcgactgatg cactgtgcaa ttacgacggt ccacagggga
     2461 aaacggagct actcacactg cttgccggaa tgctgcgcga gaagttgggt tgggatatcg
     2521 aagcacagaa tattgcacta acaaacggca gccagagcgc gtttttctac ttatttaatc
     2581 tgtttgccgg acgccgtgcc gatggtcggg tcaaaaaagt gctgttcccg ctggcaccgg
     2641 aatacattgg ctacgctgac gccggactgg aagaagatct gtttgtctct gcgcgtccga
     2701 atattgaact gctgccggaa ggccagttta aataccacgt cgattttgag catctgcata
     2761 ttggcgaaga aaccgggatg atttgcgtct cccggccgac gaatccaaca ggcaatgtga
     2821 ttactgacga agagttgctg aagcttgacg cgctggcgaa tcaacacggc attccgctgg
     2881 tgattgataa cgcttatggc gtcccgttcc cgggtatcat cttcagtgaa gcgcgcccgc
     2941 tatggaatcc gaatatcgtg ctgtgcatga gtctttccaa gctggggctg cctggctccc
     3001 gctgcggcat tatcatcgcc aatgaaaaaa tcatcaccgc catcaccaat atgaacggca
     3061 ttatcagcct ggcacctggc ggtattggtc cggcgatgat gtgtgaaatg attaagcgta
     3121 acgatctgct gcgcctgtct gaaacagtca tcaaaccgtt ttactaccag cgtgttcagg
     3181 aaactatcgc catcattcgc cgctatttac cggaagatcg ctgcctaatt cataaaccag
     3241 aaggagccat tttcctctgg ctttggttta aggatttgcc cattacgacc gagcagctct
     3301 atcagcgcct gaaagcacgc ggcgtgctga tggtgccggg gcacaacttc ttcccagggc
     3361 tggacaaacc gtggccgcat acgcatcaat gtatgcgcat gaactacgta ccagagccgg
     3421 agaaaattga ggcgggggtg aagattctgg cggaagagat agaaagagcc tgggctgaaa
     3481 gtcactaaaa attgcctgat gcgctacgct tatcaggcct acatgatttc tgcaatatat
     3541 ttaatttgca agaatttgtg ggccggataa ggcgtttacg ccgcatctgg cataaacaaa
     3601 gcgcactatt ccggcgttga gaaacgccgg aaaaacgttt caaacaggct gctgccgtag
     3661 ccgatgccgc tgcactttct cgacatccat gcactgcaac gcctttgtcg ggcaggcttc
     3721 aacacaggcc ggtcccgtct cccgatgcca gcacagatcg cacttaatcg ccctcgcctt
     3781 tttgcgcgaa gaaacgacct ccatcgcacc aaacgggcaa gccagcatac agcttttaca
     3841 gccaatgcaa cgtgtttgtt caacgaaaat atgcccatgt tcgcggctta tcgcgtcaac
     3901 agggcagaca ttcgcgcacg gtgcatcttc acactgatga caggctactg ccgtggtcca
     3961 gctgtggtct ttaatgacac gaatacggga aataaactcg tctggtgaca acgcagcgca
     4021 atcctgattc tcctgatgcg acactgcgca agccacttca caggtacggc aaccgataca
     4081 tttcgtcgca tccgcaataa taaaccggtt catgatcact cctttgcact aaaacagcag
     4141 agtgccagag agcgcgtccc tactgctttg atccaacgaa ggaaatgtta aagttttggc
     4201 acttgtccaa agcgttatgt gatcgcgccc tggtcgtccc tgacagtaaa tcccagttca
     4261 ttagaaatag cctgcgcggt ttcacgcagt ggtttcagga gatttttctc ccccacctgt
     4321 ttcagacgtg atgtcgaaag cgaaatcgcc ccggcactac gaattgatat gttccttgct
     4381 gtaacgccgc ttccacgctg ctggcgttaa accggtgtgt ttgcgaaaaa tctgccggaa
     4441 atagccaacg tcattaaacc cgcacaacgc cgccacttcc gtgagcgatc gcgcttcgct
     4501 aattaacagc ttttcggctt cgcgcacccg ctggcgatgg attgcttctg ttaacgtcag
     4561 atgaaacacc cgccggtaga cgcgcccaag ataatcggca ttgcagtaca gctcgcgggc
     4621 cagaagcgcc gtagaaagcg ggcgatgata ttgcgtgcgg atcagtttct gcgctttcca
     4681 ggcaagggtg gtgcctgcgc catcggcctg ttcatcaaga cgcgcgtcac gggtgagctg
     4741 ctgcaaaatg agcagcacaa taaactccag cgccggagtg cgttgatgct tttcctgttc
     4801 gcttaagaac tggcgaaaca gggccaccag cgcctgcgga tcgacaacct tcgtttgttg
     4861 cggcacgcta agatgagtaa gccagcgagg gtcgttgtcc ggcgctttaa cctcaaaatg
     4921 cagccagtaa aatttgagat cggcgggaaa atcctccacc ccaacatgtt gtcggttcgg
     4981 ccagagtaat aaactctctc ccgcatcgac cagaaatatc cgttcttctt cctgaatcgt
     5041 taatcgacct ttttcgacaa agataatttc ccatgactcc agccgccttg cagaatgcct
     5101 ccctacgcca cgggaaataa ataatccacc attttgcact ttaatcggta acgtcatgga
     5161 taattc
//
LOCUS       DAZKFZ010000265         5120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000265 DAZKFZ010000000
VERSION     DAZKFZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            222..1154
                     /gene="rfaD"
                     /locus_tag="ACHOQW_004399"
     CDS             222..1154
                     /gene="rfaD"
                     /locus_tag="ACHOQW_004399"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP9575348.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1164..2210
                     /gene="rfaF"
                     /locus_tag="ACHOQW_004400"
     CDS             1164..2210
                     /gene="rfaF"
                     /locus_tag="ACHOQW_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP9575349.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2214..3206
                     /gene="rfaC"
                     /locus_tag="ACHOQW_004401"
     CDS             2214..3206
                     /gene="rfaC"
                     /locus_tag="ACHOQW_004401"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP9575350.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3203..4411
                     /gene="rfaL"
                     /locus_tag="ACHOQW_004402"
     CDS             3203..4411
                     /gene="rfaL"
                     /locus_tag="ACHOQW_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP9575351.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4447..>5120)
                     /locus_tag="ACHOQW_004403"
     CDS             complement(4447..>5120)
                     /locus_tag="ACHOQW_004403"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9575352.1"
                     /translation="LANADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGR
                     IVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRC
                     IMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVK
                     ENTTGFHLKEPMTADSISSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVI
                     HNWFE"
BASE COUNT         1381 a         1150 c         1185 g         1404 t
ORIGIN      
        1 cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac atgcaaaacc
       61 aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt agcatccttg
      121 cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca gacattcgtg
      181 tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg
      241 gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc atcaccgata
      301 ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctgaata
      361 tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg
      421 gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca
      481 agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac tgcctggagc
      541 gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc acctccgact
      601 ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca aaattcctgt
      661 ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt ggcttccgct
      721 atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc
      781 atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact
      841 ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg ttcctggaaa
      901 atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc caggctgtag
      961 ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg ttcccggata
     1021 agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg cgcgcggcgg
     1081 gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg gcctggctga
     1141 atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg
     1201 acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa
     1261 tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg
     1321 aagctattcc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg
     1381 gtcatagtct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac tccttcaaat
     1441 ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc
     1501 gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg
     1561 aacgctatgt cgcgctggcc tatgacaaag gcattatgcg cacagcacaa gatctgccgc
     1621 agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc
     1681 aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg gagtttggtc
     1741 cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg attgatgaag
     1801 gttatcaggt ggttctgttt ggctctgcga aagatcatga agcaggcaat gagattcttg
     1861 ccgctttgaa taccgagcag caggcatggt gtcggaacct ggcgggggaa acacagcttg
     1921 atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat tctggcctga
     1981 tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtccg agtagcccgg
     2041 acttcacacc gccgctatcc cataaagcgc gcgtgatccg cctgattacc ggctatcaca
     2101 aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc
     2161 agcgcgtact gaaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg
     2221 ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg
     2281 atgcccagca ggcaatccca gaaattaagt ttgactgggt ggtggaagaa gggttcgcac
     2341 agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata cgtcgctggc
     2401 gtaaagcctg gttctcggcc cctataaaag ctgaacgcaa agcgtttcgt gaagcgctac
     2461 aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcgctgg
     2521 ttacgcgtct ggcacatggc gtaaagcatg gcatggactg gcaaaccgct cgcgaacctt
     2581 tagccagcct gttttacaat cgtaagcatc atattgcaaa acagcagcac gccgtagaac
     2641 gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc cagggcgatt
     2701 atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat
     2761 ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga
     2821 ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg
     2881 aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga
     2941 gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg
     3001 ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat ggaccaaccg
     3061 atccgggatt aattggtggg tatggggaga atcaagtaga gtgtcgttct acaagtatgt
     3121 ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa ataataacca
     3181 ataagttgac atcggagata agatgacctc aacattattt ttctctctcg agaaaaaaaa
     3241 ctggatagcg tactggaaca gagctctcgt attcttattc attaccacct attttttggg
     3301 tgggattaca aggtataaac atcttattgt tattcttatg acaataacga caatcgtcta
     3361 tctctgcaaa cggccaaaac actatctctc actatttaaa acatttcttt ttggtagtgt
     3421 tgccatatta actattgctg cattgctgtc acttcttcaa tcccctgatg caggtgctag
     3481 catgaaggaa attttcaaag ctattattga gaatacttta ctatgcacaa tagcaatacc
     3541 gatcatattg agagacgaga aaagagaaga tgtcgaaaaa atcgttttct tctcatttat
     3601 tagtgcgttg ggcttacgct gtttttctga attgattacc tattataagg actatcaaca
     3661 agggataatg ccattcgcag attatagaca ccgtagcatt tctgactcga tggtcttttt
     3721 attccctgca ttgttaaatc tctggcttat caaatcagca aaataccgca tttcttttgt
     3781 ggttctaagc gttattttta tttttctgat attaggaact ttatccagag gggcctggct
     3841 ttccgtgtta gtcattggat taatatggat tctgatgttt aaacaatgga agttactatt
     3901 agtaggagta atggttgcca tcattgcatt gtcggttatt ttcacacata aggagatgac
     3961 cgcaaagcta acgtataaac ttcaacaaac taatagttct tatcgctatg caaatggtac
     4021 tcaaggcagc gcactcgatc taatattaga aaatcctgtt attggttatg gttacggtaa
     4081 cgttgcatat aaagatgtct ataataaacg tgtcattgat tatccagaat ggacctttag
     4141 acaatcaata gggccacata attttgcgct attcatctgg tttggcactg gtttattagg
     4201 gctggtaagt cttatgatgc tatactgtgc aatattgaaa gagtgtataa aaaatggcgt
     4261 caagaataaa tatcgctcac catataatgc atattatata atcttactat cttttatagg
     4321 ttattttgtt atccgtggaa acgtagaaca aattgaacca aatttattag gcgtttacgc
     4381 cggcttatta ttagcgatga aaaacaagta aaaaataaaa aaggctgcat aatgcggcct
     4441 ttttatttat tcaaaccaat tatgaataac ctcttcgaat ctttgagtta cgccatccca
     4501 actgtagtga tcaaacacaa agtcctgacc ttgctttgca actgctgtta attcaggatt
     4561 cgctaacgtt tttaaaatat cgctgcttat ggagtctgca gtcataggtt cttttaaatg
     4621 gaatcccgta gtattttctt tgacaaattc cgtcatcccc ccacgcgtac tcactaaaac
     4681 aggcttacca gcacccatag cttcaattgc caccatgcaa aatggttcct ggaactggga
     4741 aggaatgacg actaaatctg ccagtgggta ataacaatgc atcttttcag gcggtatgct
     4801 tcctaacata atgcaacgat ccttcaaacg ttttgcaatt tccctgacat tgcgttgata
     4861 tgcacctttg tcacttttac tcatttctgt ataatcacca atcacaacta attttaaatt
     4921 tttgtgagca gcggccaatt tttcgaatga ttgcatcaac agcaatatgc ctttgtcagg
     4981 gacgatacgc ccagcataaa aaataatttt ctcttcaggg gagatattaa gttcgcttct
     5041 agtgatcggc tgaaaattag attgataggt ttctaaatca attccatttg gtacaatctc
     5101 aatatctgca tttgctaaat
//
LOCUS       DAZKFZ010000266         5085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000266 DAZKFZ010000000
VERSION     DAZKFZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>5085
                     /locus_tag="ACHOQW_004404"
     CDS             <1..>5085
                     /locus_tag="ACHOQW_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9575353.1"
                     /translation="EDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDS
                     QPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSG
                     LTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATV
                     ADTEGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKT"
BASE COUNT         1476 a         1272 c         1283 g         1054 t
ORIGIN      
        1 aggaagacaa cacggttgaa gtcacccttg aaaatggcgt gaaacaaacg ttaatcgtca
       61 gttttgtcgg cgactcgagt accgcgcagg ttgatctgca gaagtcgaaa aatgaagtgg
      121 tcgctgacgg caatgacagt gccacaatga ccgcgacagt tcgggatgca aaaggcaacc
      181 tgctcaatga cgtcaaggtc accttcaatg tcaattcagc agcagcgaaa ctgagccaaa
      241 ccgaagtgaa tagccacgac gggatcgcca cagctacgct gaccagtttg aaaaatggtg
      301 attatacggt tacggcctct gtgagctctg gttctcaggc taatcaacag gtgattttta
      361 tcggtgatca aagtactgct gccctgaccc tcagtgtgcc ttcaggtgat atcaccgtca
      421 ccaacacagc tccgctacat atgactgcaa ccttgcagga taaaaatggc aatccactaa
      481 aagataaaga aatcaccttc tctgtgccaa acgacgtcgc aagtcggttc tcgattagca
      541 acagcggaaa aggcatgacg gatagcaacg ggactgcaat cgcctccctg accggcacgt
      601 tagcgggcac gcatatgatc acggctcgtc tggctaacag caatgtcagc gatacacagc
      661 caatgacgtt tgtggcggat aaagacagag cggttgtcgt tctgcaaaca tcgaaagcgg
      721 aaatcattgg gaatggcgtg gatgagacga ctctgacagc aacagttaaa gatccttttg
      781 ataacgtggt taaaaatctt tcagtagtct tccgcacctc ccccgcagac acgcaactga
      841 gtctgaacgc gcgtaatact aatgagaacg gtattgccga agttaccctt aagggcacgg
      901 ttttgggtgt tcatacagcc gaagccatac tgcttaacgg caacagagat acgaaaatcg
      961 tcaatattgc gcccgatgcc agcaacgcgc aggtcaccct gaacatccct gcacaacagg
     1021 tggtgacgaa taacagtgac agcgtgcagc tgacggcgac ggtgaaagac ccgtcgaatc
     1081 atccggtggc gggaataacg gtgaacttca ccatgccaca ggacgtggcg gcaaacttta
     1141 cccttgaaaa taacggtatt gccatcactc aggccaatgg cgaagcgcat gtcaccctca
     1201 aaggcaaaaa agcgggcacg catactgtga ccgccacgct gggtaacaat aatgccagcg
     1261 atgcgcaacc agtcaccttc gtggcggata aggacagcgc ggttgtcgtt ctgcaaacat
     1321 cgaaagcgga aatcattggg aatggcgtgg atgagacgac tctgacggca acagtgaaag
     1381 atccttttga taacgcagta aaagatctac aggtcacctt cagtaccaac cccgcagata
     1441 ctcaacttag tcagagcaaa agcaatacta acgacagtgg tgtggccgaa gttaccttta
     1501 agggcacggt tttgggtgtt catacagccg aagccacact gcctaacggc aacaacgata
     1561 cgaagatagt caatattgcg cccgatgcca gcaacgcgca ggttacgctg aacatccctg
     1621 ctcaacaggt ggtgacgaat aacagcgaca gcgtgcagct gacggcgacg gtgaaagatc
     1681 cgtcgaatca tccggtggcg ggaataacgg tgaacttcac catgccacag gacgtggcgg
     1741 caaactttac cctcgaaaat aacggtattg ccatcaccca ggccaatggg gaagcgcatg
     1801 tcacgctcaa aggtaaaaaa gcgggtacgc atacggttac cgcaacgctg agtaataaca
     1861 ataccagtga ttcacagccg gtaacgtttg tggcggacaa aacctcggct ctggttgttc
     1921 ttcagatatc aaaaaatgag atcacaggta atggcgtcga tagcgcaacg ctaactgcaa
     1981 cggtcaaaga tcagttcgac aatgaggtga acaatcttcc ggtaacattc agcacagctt
     2041 cttcaggcct caccctgacc ccaggggaaa gtaataccaa tgagtctggc atcgcgcagg
     2101 ccactctcgc aggcgttgcc tttggtgagc agacggtcac tgcatcactg gctaataatg
     2161 gtgccagcga caacaaaact gtgcatttta ttggcgacac agcggcggca aaaattatcg
     2221 agttgacgcc tgtcccagac agcataatcg caggtacccc gcagaacagc tccggcagcg
     2281 tcatcaccgc cacagtcgtt gataataatg gctttccggt gaaaggtgtg actgtgaact
     2341 tcaccagcaa cgcagcgaca gccgaaatga cgaatggcgg tcaagccgtg acgaacgaac
     2401 agggtaaggc taccgtcact tataccaata cccgctcctc gatagaatca ggagcgagac
     2461 cggataccgt tgaggccagt ctggaaaatg gtagctccac gcttagcaca tcaattaatg
     2521 tcaacgctga tgcgtctacg gcacatctca ccttgctaca ggcacttttt gatacagtct
     2581 ccgcaggcga cactaccaat ctgtatattg aggtgaagga taattacggc aacggagtac
     2641 cccagcagga ggtaaccctc agcgtttcac caagtgaagg tgtgaccccc agtaataacg
     2701 ctatatatac gaccaatcac gacggcaatt tttacgcaag ctttaccgct acaaaagccg
     2761 gggtatacca agtgacggca accctcgaaa atggcgattc gatgcaacaa acagtgacct
     2821 atgtgccgaa cgtagcgaat gctgaaatct cgctggcagc ctcgaaggat ccggtaattg
     2881 ccaacaataa cgatctcacg acactaacag caacagtcgc tgatacagag ggcaatgcga
     2941 tagccaacag tgaggtaaca tttactctgc cggaagatgt gagggcgaac ttcacgctgg
     3001 gcgatggcgg taaagtggtt actgatactg aaggcaaagc gaaagtcacg ctgaaaggta
     3061 caaaagcagg cgctcatact gttacagcat cgatggctgg cggtaagagt gagcagttgg
     3121 tggtgaactt tattgcggat acactcactg cgcaggttaa tcttaacgtt accgaggaca
     3181 attttatcgc taataacgtc gggatgacca ggctgcaggc aacagtgact gatggaaacg
     3241 gcaacccgtt agccaatgag gcggtgacat tcacgctacc ggcagatgtg agcgcaagct
     3301 ttactctcgg acaaggcggt tccgccatta ctgacatcaa cggcaaggct gaagttacac
     3361 tgagcggtac aaaatccggc acctaccccg tgacagttag cgtgaacaat tatggtgtca
     3421 gtgatacgaa acaggtgact ttgattgccg atgctggtac cgcaaaacta gcctccttaa
     3481 cctctgtata ctcattcgtc gtcagcacga ccgagggcgc gaccatgact gcaagcgtca
     3541 ctgacgctaa cggcaacccg gtagaaggta taaaagttaa tttccgcgga acttccgtca
     3601 cgctaagcag caccagcgtt gaaacggatg atcggggttt cgctgaaatt cttgtgacaa
     3661 gcaccgaggt cggactgaaa acagtttcag cctctctggc agataaacct actgaagtca
     3721 tctcgcgatt actgaatgca aaagcagata ttaattctgc aacgattacc agtctggaga
     3781 tacctgaagg tcaggtcatg gtcgcacaag acgtagcagt taaagctcac gtcaacgacc
     3841 agtttggcaa tccgattctt aatgaatctg taacattcag tgcagaacca ccagagcaca
     3901 tgaccatcag ccaaaatatt gtctctactg atacgcatgg tatagccgag gtcactatga
     3961 cgcccgaaag aaacggttcg tatatggtga aagcatccct ggcgaatgga tcctcttatg
     4021 agaaggatct ggtggtaatc gatcaaaaac tgacactctc ggcgtccagc ccgcttatcg
     4081 gtgtcaattc cccaacaggt gcaactctga cggcaacgct aacttctgca aatggcactc
     4141 cagtggaggg tcaggtcatc aactttagcg taacgccaga aggtgcgacg ttaagtggcg
     4201 gaaaagtgag aaccaactct tcaggtcagg ctccagtcgt tctgaccagc aataaagtcg
     4261 gtacatatac ggtgactgca tcgttccata acggcgtaac aatacagaca cagacaatcg
     4321 tgaaagtcac tggcaactca agcaccgccc atgttgctag ctttatcgct gatccatcga
     4381 ctatagccgc caccaacagt gatttaagta ccttaaaggc aacggttgag gatggcagtg
     4441 gtaacctgat cgaaggtctc actgtgtact tcgccttaaa aagcggctct gccacattaa
     4501 cgtcattaac agcggtgaca gatcaaaacg gaatcgcgac aacaagcgtg agaggagcga
     4561 taacggggag cgtcacggta agcgcagtca cgaccgctgg tggaatgcaa acagtagata
     4621 taacgctggt ggcaggcccg gcagacgcct cgcagtccgt ccttaagaac aatcggtcat
     4681 cattgaaagg agactttacc gatagtgctg agctacatct tgttctgcac gatatatcag
     4741 gcaatccgat caaagtttct gaagggctgg aatttgtgca gtcaggtacc aacgcgccct
     4801 atgtgcaagt tagtgcaatt gactacagta aaaatttctc aggcgagtac aaagccactg
     4861 ttacaggcgg cggagagggt atcgcaacgc tgatccctgt attgaatggt gttcatcaag
     4921 cgggtctgag taccacaata caattcactc gcgcagaaga caaaataatg agcggtacag
     4981 tgttagtcaa tggtgctaac ctaccgacaa ctacattccc ttcgcagggg ttcactgggg
     5041 cgtattatca gttgaataat gacaactttg ccccaggaaa aacgg
//
LOCUS       DAZKFZ010000267         5073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000267 DAZKFZ010000000
VERSION     DAZKFZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..1051
                     /gene="tsuA"
                     /locus_tag="ACHOQW_004405"
     CDS             2..1051
                     /gene="tsuA"
                     /locus_tag="ACHOQW_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP9575354.1"
                     /translation="MILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLI
                     AISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEG
                     LIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVLLVI
                     TLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEAT
                     GRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADA
                     QTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASWLVY
                     VRPQRKARLATAAAN"
     gene            1065..1292
                     /gene="tsuB"
                     /locus_tag="ACHOQW_004406"
     CDS             1065..1292
                     /gene="tsuB"
                     /locus_tag="ACHOQW_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP9575355.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1335..2759)
                     /gene="sbcB"
                     /locus_tag="ACHOQW_004407"
     CDS             complement(1335..2759)
                     /gene="sbcB"
                     /locus_tag="ACHOQW_004407"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP9575356.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2941..4119
                     /locus_tag="ACHOQW_004408"
     CDS             2941..4119
                     /locus_tag="ACHOQW_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9575357.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     DQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            complement(4100..4291)
                     /locus_tag="ACHOQW_004409"
     CDS             complement(4100..4291)
                     /locus_tag="ACHOQW_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9575358.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(4369..4713)
                     /locus_tag="ACHOQW_004410"
     CDS             complement(4369..4713)
                     /locus_tag="ACHOQW_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575359.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHGFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT         1247 a         1204 c         1279 g         1343 t
ORIGIN      
        1 aatgatatta agcgggctaa tttgtggtgc tctgctggga tttgtcatgc agcgtgggcg
       61 tttttgcctg actggtggtt ttcgcgatat gtatatcgcg aaaaataatc gcatgtttta
      121 cgccttgctg attgcaattt cggtacaaag cgtgggggtt tttgcgttaa ttcaggcggg
      181 cctactgact tacgaagccg gggcgttccc gtggcttggt actgttatag gtgggtatct
      241 cttcgggctg ggaattgttc tggcgggtgg atgtgccacc gggacctggt atcgcgcggg
      301 tgagggattg atcggcagtt ggatcgcgct tttcacttat atggtgatga gtgcggtgat
      361 gcgttctcca cacgccagtg gtttaaatca aaccttgcag cactacacta ctgaacataa
      421 ctctattgct gagactttta atttgtctgt gtggccgttg gttgccgttt tgctggtgat
      481 aacgctctgg gtggtgatga aagagttgaa gaagccaaaa ctcaaagtcg cgaccttacc
      541 gccgcgccga accgggatcg ctcatattct gtttgaaaaa cgctggcatc cctttgtcac
      601 agctgtactc atcggtttga ttgcgctttt agcctggccc ctgagtgaag caaccggacg
      661 tatgtttggg ttaggcatca cttccccaac ggccaatatt ctgcaatttc tggtcgcggg
      721 tgacgtgaaa tacattaact ggggcgtttt cctggtgtta gggatcttcg tgggggcatt
      781 tattgcagcc aaagcgagcc gtgagttccg cgttcgcgca gctgatgcac aaacaacagt
      841 acgtagcggg ctaggtggtg tactgatggg cttcggtgcc agtattgcag gtggttgctc
      901 tatcggtaat ggactggtta tgactgcaat gatgacctgg cagggctggg ttggccttgt
      961 atttatgatt ctcggagtct ggactgcgtc ctggcttgtg tatgttcgac cgcagcgtaa
     1021 ggcgcgactg gcaactgctg cggcaaatta agtaaggtgt aaagatggcg attaaaaagc
     1081 tggatgtggt gacgcaggtc tgtccgtttc cactcataga agcaaaagca gcactggcag
     1141 aaatggccag cggcgatgag ttggtaattg agtttgattg cacccaggca actgaagcaa
     1201 tcccacagtg ggcggcagaa gaagggcatg ccatcaccga ttatcagcag attggcgatg
     1261 cagcgtggag catcaccgtt caaaaagcct gactcaacat tgtcctccgc cgtaccagcg
     1321 gcggaggctt cacattagac aatctcttcc gcgtactgcc aaagcgcttt taacagcgcc
     1381 actttttctt tatcgtcggc atactgctgc gccagcatct gaatctcttc tgcatagcct
     1441 tgtaaaaact ctggcgtgaa gacctggcgg cggtgctcca gccagcgttg ctgctcggca
     1501 taatccagcg tccccgggaa gttgcgtgcc cgataattga acaacagctt ttcaatccgt
     1561 ttatcaacaa aagtgatatc cagtgccggt aaattacgcg gctcggtttc aagcacaatt
     1621 ttcattgctg cacgatcggc gtcactgaaa aagccattat aaagctgtgc atccacgtta
     1681 tctgaaggcg taaacggttc ggcttccgcg aatatcgcca ccactttttc acgcacttgc
     1741 ggattttcac gcagaatttt caggttatcg aggcaatgct gacgattaat tcccagtcgg
     1801 tcggcatctt ccgggcgtag cgtattcgcc tgggccagca ccggacattt attgatatgt
     1861 accagtttaa ccggaacggc ggcgttatcg ccaagatcgg tttttgcggt atataaacgc
     1921 tcgcgcaatg tgtcgctatc cagttccagt aatggcgaaa tatctcctgc caaatccacc
     1981 ataattacgg cattgcgatt ttcaggatgc cacgccagcg gtgccaccca gctggtattg
     2041 ccgcgccatg ccccaaacat tccggaaacg tgaaccaggg gtttcatctg cggaacatca
     2101 atcaacgcca tcagtttgtg tttattacga tgggtaaaga gataatcaaa caggcgtggt
     2161 tgacgcgttt ttaccagttt cgccatcgca atagtggcgt acacatcagc catcgcatcg
     2221 tgggcgttgc tatgttcaat accattcgct ttggttaaat gctcaaggcg aaagctcggt
     2281 agaccgtcat cattttcagg ccagtttatt ccttccgggc gcagggcata gcaggcacgc
     2341 ataacatcca gtaaatccca gcgcgagtta tcatgctgcc agctccaggc gtaaggatcg
     2401 tagaaattac gataaaaaac gttgcgtgtg acttcgtcgt cgaaacgcac attgttgtag
     2461 cccagaatac aggtcttcgg tacggtaaaa agcgagtgaa tacgggcggc aaatgcggct
     2521 tcgttttctc ctttcgcccg agcttcctgc ggggtaatac cggtaattaa tactgctcca
     2581 ggctggggta aatagtcatc cgcgggcttg cagtaaaaga cttcgggttc gccgatgaca
     2641 ttgaattcgt catcggtgcg aatggctgcg aactgtgcag ggcgatctaa cgcggggtgc
     2701 gtgccaaagg tttcgtaatc gtgaaagagg aaggtaggtt gcttatctgt gtctgtcatt
     2761 gtctagcatt gtccaatgta gttaattaac tgtctgttat aactcgaaaa acaacccttt
     2821 tttcacctga gtatgtctac gaacgtcaaa tatagttgtt tactgtgcgc tgttgtccat
     2881 gtgatattgt gtaccagatg tgtaccaaac cattatttct gagcgtacca aatattattt
     2941 atggctataa gcgacacaaa actgcgtact atttatggta aaccatattc tggcccacaa
     3001 gaagtggctg atgccgatgg cctcagcgta cgaatttcac cgaagggggt catccagttc
     3061 cagtaccgct atcgctggca tggcaagcct aatcgacttg ggcttggtcg atacccatcc
     3121 ctgtctttga aggatgccag acagatcact gctgacttgc gaaagctcta tttctcagga
     3181 acggatccac gcacttattt tgaagagaag gtggagaact ccatgacggt cgcccagtgt
     3241 ctcgactact ggttcgacaa ctacgtctct acaactctca gagaaaagac ccaagcactt
     3301 taccgatcaa cggttatgaa gcgcatgcat gacgccttcc ctaatcgtcc ggcatcttct
     3361 atcacggtta agcaatgggt tgacctgctt accgaagaag aaaaagataa tccacgccga
     3421 gcaaggcagg tgctaagtca actaagatca gcaattagtt ggtgcatgcg gcgtcagttg
     3481 atagatagtt gcgcaattat gagcatccaa ccaagggact tcggctcccg cgctgaggta
     3541 ggggatcggg tactgtcgta tcacgaactg gctaagatct ggcttgctat tgaaagaagc
     3601 cgtgcgtcta cgtcaaataa gctccttcat cagatgctta tgctgtgggg ggcgaggctc
     3661 tcagagctta ggctggcaac aaagacagaa tttgacctgc tggacaacgt atggaccgta
     3721 ccgaaagagc atagcaagat gggtaatgtt atccgccgtc caatcttcga tcaaattaag
     3781 ccttttctcg aaaaggccat gacaacgtac aatgatgttc ttttccctgg agaagacata
     3841 aacaaaccga tcagcatcgc tgcagctaac cgattcgtaa atagaataag gggagggatg
     3901 gatctaggtt actggcgaac acatgatttc agaagaacgc ttgttacacg tctgtccgag
     3961 atgaatgtcg agcctcatgt tactgagcga atgctcggtc atgaacttgg cgggataatg
     4021 tccgtataca ataaacacga ctggatagag gctcagcgca aagcgtatga gcttcacgct
     4081 gataaattgt tctggcacat caggagcatt tctgattaac gccaccgtta agaatccacc
     4141 cttcaacagc ttcacgaagg tatgatttgg ggtgggttct gactggcttc ggaaatccgt
     4201 gccgtttggt atagttccag attgtctgac gtgatgaaac accgagcttg ttcatcactt
     4261 ctttctcagg aatcaggctg gtatcggtca tcttaattct ccaggcaaaa agaaaccgcc
     4321 atatagcggc tctatcagat atgaacaggc ctcatcgagt gtgaggcgtt agtccttgcg
     4381 tagctcgctg attcttctgt aagtctctgg tgctttgttt ccgtgtatct tcatttcaga
     4441 cttcaacaga gcaacgaggg aatcccattc gttgaggatt cctttgaatg ccggaacgcg
     4501 ctttgcaacc ttgtcgaatg aatctctgat ttctggaatc tgctcaacaa gtgcaacgca
     4561 tcgtctgaaa tcggctgcgt catgtggagc accgaagcca tgaccataga tattcttttt
     4621 caggccacat gcgattgagg caagagttgc gctactgatg ccgacatcgc cagttgattg
     4681 ccatttcaaa accttcatag ccaaatctga catttcttgt ctccataaaa caaaactcgc
     4741 cgtagcgagt tcagataaaa gaaatccccg cgagtgcgag gattgttagt tgcgctctgc
     4801 tgctgcctta gccattacca tatccacaca atttcaccag acatgattct cgcaatcact
     4861 atcatcacca aagtgataat cacaacttta actggcggca tcattcacca tcctgctgcg
     4921 gtgctgctgg atatactttt ctcatccaat gcgtggcgtc atgaataacg aactgatatt
     4981 catcatcagt aaacatcgag ccatcccact gtagcccgtc aagaacacct ctggatggag
     5041 agaataccca gatatcgaaa tcgtatttct ccg
//
LOCUS       DAZKFZ010000268         5043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000268 DAZKFZ010000000
VERSION     DAZKFZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHOQW_004411"
     CDS             <1..384
                     /locus_tag="ACHOQW_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9575360.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ACHOQW_004412"
     CDS             419..1117
                     /locus_tag="ACHOQW_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9575361.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ACHOQW_004413"
     CDS             1139..1363
                     /locus_tag="ACHOQW_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575362.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ACHOQW_004414"
     CDS             1360..1716
                     /locus_tag="ACHOQW_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFP9575363.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ACHOQW_004415"
     CDS             1749..1901
                     /locus_tag="ACHOQW_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575364.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ACHOQW_004416"
     CDS             1898..2209
                     /locus_tag="ACHOQW_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575365.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="ACHOQW_004417"
     CDS             2336..2899
                     /locus_tag="ACHOQW_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575366.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="ACHOQW_004418"
     CDS             3009..3113
                     /locus_tag="ACHOQW_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575367.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="ACHOQW_004419"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="ACHOQW_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9575368.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="ACHOQW_004420"
     CDS             3680..3958
                     /locus_tag="ACHOQW_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575369.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="ACHOQW_004421"
     CDS             3960..5009
                     /locus_tag="ACHOQW_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9575370.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
BASE COUNT         1356 a         1132 c         1408 g         1147 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggccacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg gggggaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc cagatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt catgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 tac
//
LOCUS       DAZKFZ010000269         5019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000269 DAZKFZ010000000
VERSION     DAZKFZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..206)
                     /locus_tag="ACHOQW_004422"
     CDS             complement(<1..206)
                     /locus_tag="ACHOQW_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9575371.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDA"
     gene            complement(190..432)
                     /locus_tag="ACHOQW_004423"
     CDS             complement(190..432)
                     /locus_tag="ACHOQW_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9575372.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            824..1162
                     /locus_tag="ACHOQW_004424"
     CDS             824..1162
                     /locus_tag="ACHOQW_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9575373.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            1455..1607
                     /locus_tag="ACHOQW_004425"
     CDS             1455..1607
                     /locus_tag="ACHOQW_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9575374.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            1619..2257
                     /locus_tag="ACHOQW_004426"
     CDS             1619..2257
                     /locus_tag="ACHOQW_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575375.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2258..2467)
                     /locus_tag="ACHOQW_004427"
     CDS             complement(2258..2467)
                     /locus_tag="ACHOQW_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575376.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            3032..3220
                     /gene="dicB"
                     /locus_tag="ACHOQW_004428"
     CDS             3032..3220
                     /gene="dicB"
                     /locus_tag="ACHOQW_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9575377.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            3217..3405
                     /locus_tag="ACHOQW_004429"
     CDS             3217..3405
                     /locus_tag="ACHOQW_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9575378.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            3498..4736
                     /locus_tag="ACHOQW_004430"
                     /pseudo
     CDS             3498..4736
                     /locus_tag="ACHOQW_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            4724..5019
                     /locus_tag="ACHOQW_004431"
                     /pseudo
     CDS             4724..5019
                     /locus_tag="ACHOQW_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1465 a         1134 c         1142 g         1278 t
ORIGIN      
        1 gcatcagggg tgtctttctc tatccagcgg aaaattttct gggtattgcg agccagggct
       61 tccggatggc tgtcgtcgta cagttcagga aacgtcatac ccaactcaaa ataagcctgg
      121 gttattccag ctgccggaac tttttcgcca tcaggacgcg cccaggcatt catcgccatg
      181 cggatgtgtt catgcttgat tttcatgaat ccccccttgg ttagaaggcg gattatgatc
      241 agaatcggga atgacaaccg tcggtatgtg taactcatat ttgagcgccc cggcagtgac
      301 tgcctgaatt agcaacgccc atttccacgg aacgtcttcc ccccacatgc tgactgtggt
      361 ttttgacgtt cctagagcgg cggctgtttt aacaactccg ccaaaatagc ctaatacttc
      421 tgattttttc atgagtcgct ccataaaact gaacgccaaa agtttaataa tcaaaaccaa
      481 agaaagtcaa gaaacaaaac catctgtgtt ttaaaatcaa aacatgaaca agcaaacaat
      541 atctgaacgc ataacccaac gtatgcatgc gctaaacttg aaaggcaaag accttgtcaa
      601 tgccactggc gcatcaaaag gctccgtaag tcaatggatg aacggtggag gagcgccgtc
      661 ctcgcgttac ataagttcac tggcaaagat attgaaagta aacgaaaatt ggcttcttaa
      721 tggaggagag ttaaatacag gtgattcgct tgatctatct ttaccgccga taaaaacggt
      781 tccgctacta tcacttcagc aggcagcaag ctggagtgat tatatgaaaa attcctcaat
      841 aacctcttgt gtgcagcttg tcggagaaat cccggccaat acctttgctg ttgttctaga
      901 gagtgacagt atgtcgacat ctggtggtgg agtttccatc ccaaatggtt caacagtttt
      961 tgttgatccc gatcgaatcg tacaaccagg aaatattgtc cttgccttac ccaaagggac
     1021 cacaacgcct gtcattcgca aactggagat agaagggccg gatattcttt tagtccccac
     1081 gaatcctcgc tacccttcaa ttatgctgga tgatctatct tgcatattgg gagtatgctt
     1141 taaaattcaa caagatattt aaccaacctc atctatttga ttaactgtat gccatcgtag
     1201 tgatggcgta acagctgcct gcttaaaatg ttttgataaa aaaacattga ccttaaatgt
     1261 tcgtttttct aaacttcatt cattccctca ccccgcccca cagaatgcag ggcaatactt
     1321 cgagttacca ggcagtggtc aggggttaag tagccagccc gaggcgtaag aacatgacgg
     1381 cagggttcaa ctttaataac tatgcagcag gtttttgttc cgctaccccg gcgttaaggg
     1441 gaaatgaggt caacatggat acgctcaatc ttggcaacaa cgaatctctg gtatgtggcg
     1501 tgttccccaa ccaggacggt acgttcaccg cgatgacgta taccaaaagc aaaacgttta
     1561 aaactgaagc tggcgcgcgt cgctggttag ccagaaacac tgactgatga ggttgacgat
     1621 ggaatttaaa gatttacctc cttcaatcca ggagattgca gcacacacac ttcgtcatcg
     1681 tctgaacgaa cttgaattgg aatcggtaac gaaaaaagac actgataata tggctcgtaa
     1741 tgtgcgcgat gcgtttaccg gattgtattt ctgtgcgtct ataaataaac acgactcaga
     1801 gagtgtggca aataaaattg cagaaacgac agcgcaaaac atcaatacga aaccaacgga
     1861 agaagaaatt gatcagtttg ctcatgatgc tggtttaaaa aacaagaaag aaaaatcgcc
     1921 atatgcgggg aacatgtttg tttatgacaa tctcatcaga attcgtggcg aaattccggc
     1981 ggaatacctg gcaagagtcc atcaggcatt gcttaaaaat ttggaaacag aattatttga
     2041 tggcaacact aacggtttct tcatggtatc aggccttgag aaagactggg atgcagaaaa
     2101 acgctggaat gttgctacat ggttattcag taacagagcc gctgcactgg aagcttcggc
     2161 atgtatttgc ggcctgttct taacagacca caaatataat ctggatgtgt acagttatat
     2221 ttacgctgaa cacggtccac tctggattga ctggtaatta taaggaaaca ccagcagggc
     2281 cgcggcgacc aacagaacga ttaaaatcaa taatgccatt ataaaggaca ttatttaatt
     2341 tatcgtcgaa tgctgattct gtgagcctca actctgaatg agtttttaat aaccctgatt
     2401 gcctgagttg atttaccagg cattcaatct gtttttcaat aagcggattt ctttttttgt
     2461 ttggcatttt atcctccatt gaggttctgg gttaaaaatg gagaccaaca cgctgtcacg
     2521 tgtggtcgtg cgccggacac ggataagaat ccggtactga cagtttactg aaaggatatt
     2581 tccctgaaaa gtcagtgcat aacgcgaaag cgtacggcga agctctttcc cttagaaggc
     2641 ttgtcgttag atttcttcga acgtgcgctt ccggttgtgg cactccgcga aatggcgcgg
     2701 cggtaagtat ggcggggtta ttccttcccc attgaggaca ccgggttgtc aggttgacca
     2761 tacgcttaag tgacaacccc gctgcaacgc cctctgttat caattttctg gtgacgtttg
     2821 gcggtatcag ttttactccg tggctgctct gccgcccttt ttaaagtgaa ttttgtgatg
     2881 cggtgaatgc ggctaagcgc acgcggaaca gttaaaacca aaaacagtgt tatgggtgga
     2941 ttctctgtat ccggcgttaa ttgttaactg gttaacgtca cctggaggca ccaggcactg
     3001 catcacaaaa ttcattgttg aggacgcgat aatggaaacg ttattaccaa acgttaatac
     3061 gtctgaaggt tgttttgata ttggtgttct gctcagtaac cgggagttta ctgaagatgc
     3121 cattaacatg aggaaatatg agccttatct gctcaatgat aattccatac tttcccgaat
     3181 tgctcttctt gaacttggta ttttcggaga acgtcaatga cttcagcatt tgcactgatg
     3241 atgacggttt ttcttataac gggtgaatca cagaatgtga ttaccggaat ttatgccagt
     3301 aaagaatcct gcctccaggc aagagacgag caaaaaattt ctggtgaatg cctcccggta
     3361 aaaaaagtat cgctgtacct gaataacgaa acaccggctg gataaccctc cagccatatt
     3421 aacaccatac caacggatta aaaatgccag caatggcagg gatttgttca cccttaaatc
     3481 tgtaatgagg tttatcaatg agcactgata aagaagaatt tgcgctatat tgcgaagcaa
     3541 aaaatgacaa agtcagaaaa cgtctgggaa ttaaaggtgg tttttactgg actacagcaa
     3601 aaaaattatc tgttgccatc tcccgctgca ttaccgcaat ggatgacaac gattatgatg
     3661 aagacgactt taaaaaaccc gttcgcgtcc atttacccgt tgtgaatgac cttccacctg
     3721 aaggcgtgtt tgataccgaa ttctgcaacc gatacgaaaa aggcggggaa gatggcatta
     3781 caatggtatt tatcgcgccc tctccctccg tgcaggagaa accagccagt actgacaata
     3841 ccaacgtcaa cggcgaagac atgacggaga ttgaggagaa tatgctcctg ccggtttctg
     3901 gtcaggagct gcccattcgc tggcttgcgc aacacggcag cgaaaaacca gtaacgcacg
     3961 ttgcacggga agaacttcag gcattacata tagcacgggc ggaagaactg ccggctgtta
     4021 ctgccctggc catttctcac aaaacaaagc tgctcgaccc gctggagatt cgcgaccttc
     4081 acaaactggt acgcgacact gacaaagttt tccctaatcc cggtaattca gacctgggac
     4141 tgataactgc ttttttcgag gcatacctgg acgctgacta cactgatcgg ggtctgctga
     4201 caaaagagtg gatgaaagga aatcgtgttt cgcgtatcac ccgtacggct tccggtgcaa
     4261 atgccggtgg cgggaacaaa accgatcgca atccgaattt agtacacacc ttcgatacgc
     4321 tggatgtgga gattgcagca gccacacttc cgatggattt taatatttat gaaattccgg
     4381 gcagcgttta tcgtcgcgca aaagaaatcg tcctgaaaag agaaagtccg ttcaaagaat
     4441 ggtccgcagc acttcgcgca accccgggta ttctggacta ttcccgcgcc gctatttttg
     4501 cacttatccg gagcgctcac cctgagtttt atcactaccc gggacgcctt caggggtata
     4561 tcaacgccta cttaacggag actgatcacg agaaccccag caaggaaact ctcactgctg
     4621 cacgacatac accggaaaaa gatatcctgg aagaagttaa ccgcgaactg tctgctaagc
     4681 aggaaacaga agaagaaaat gatgaagaaa aaccgcaacc atcttgcgca atggcagaat
     4741 gataatcaga agaaatctgg caaaagaccg ttttacagta ccaaaaacac aggcgggaac
     4801 caatcgtgtg attcacctta ttaagcccgc aatcgacgct ctccggagtc agatggcact
     4861 aacgagactg agcaaagagc atatcatcga tgttcacctc agagaattcg gcagaacaga
     4921 gaaacaaaaa tgtacctttg tttttcaacc tgaagtgtca gcgaaagtaa aaaattatgg
     4981 cgaccatttt accgttgact caataaggca gatgtggga
//
LOCUS       DAZKFZ010000270         5012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000270 DAZKFZ010000000
VERSION     DAZKFZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            95..3463
                     /locus_tag="ACHOQW_004432"
                     /pseudo
     CDS             95..3463
                     /locus_tag="ACHOQW_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            3466..4392
                     /gene="hipA"
                     /locus_tag="ACHOQW_004433"
                     /pseudo
     CDS             3466..4392
                     /gene="hipA"
                     /locus_tag="ACHOQW_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            4663..4932
                     /locus_tag="ACHOQW_004434"
                     /pseudo
     CDS             4663..4932
                     /locus_tag="ACHOQW_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1349 a         1021 c         1377 g         1265 t
ORIGIN      
        1 caatacagac ctctgacaat ttaataaatt tcatttttaa tgtctgacga ctcatctctt
       61 tcgtggctgt attgtttgta tttggagcgt ttatatgaat agaatctatc gcgtgatatg
      121 gaattgcact ctacaggtat ttcaggcctg ctcggaatta actcgcaggg taggtaaaac
      181 atcaacggtt aatttgcgaa agtcctctgg actgacaacg aaattcagta gattgacgct
      241 gggtgttttg ctggcactaa gcggttcagt gtctggtgcg agtctggaag ttgataatgg
      301 tcagattacc aatattgata ctgatgttgc ttatgatgcc tacctggttg gctggtatgg
      361 cactggagtg cttaatattt tggctggcgg taatgcctcc ttaaccacta ttactaccag
      421 cgtcattggc ggtaatgagg attcggaggg taccgttaat gttttgggtg gcacctggcg
      481 attgtatgat agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg gaacggggac
      541 gctgaatatt aaacagaagg gtcacgtcga tggaggctat ttaagattag gtactcaagc
      601 tgcaggcgtc gggacggtta atgttgaggg agaggactcg gttttgacga ccgaattatt
      661 cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt acgtcacgag
      721 ttcaatagtc gccattttag gatatcaagc gaacagtaat ggtaaggttg tcgttgaaaa
      781 gggtggcgag tggctaataa aaaataatga ttcctcaatt gaatttcaaa ttggtaatca
      841 aggaactggg gaggcgacta ttcgcgaggg tgggctgatt acggctgaaa atacgattat
      901 cggtggcaat gccaccggtg tcggaaccct gaatgtgcag gatcaagact ctgtcatcac
      961 ggtacgcaga ctctataatg gatatttcgg taatggcgca gtcaatattt ccaataatgg
     1021 actaattaat aacaaagaat attcattggt gggcgttcag gacggttccc acggtgtcgt
     1081 caacgtgacc gataaagggc attggaattt cctcggaacg ggcgaagctt tccgctatat
     1141 ctatatcggt gatgctggcg acggtgaact taatgtctcg cgtgaaggta aagtagattc
     1201 gggaattatc actgcgggga tgaaagaaac aggcacaggc aaccttactg ttaaggataa
     1261 gaactccgtt atcactaatc tcggaactaa tcttggttat gacggccacg gcgaaatgaa
     1321 tatcagtaat gaggggcttg ttgtcagcaa cggaggaagc tctctcggtt atggagaaac
     1381 cggcgtcggg aaggttagca tcaccacggg ggggatatgg gaggtcaata agaatgtcta
     1441 taccaccatt ggtgttgcgg gcgtcggaaa cctcaatatt agcgatggcg gtaagttcgt
     1501 atcgcaaaat attacttttt tgggcgataa agcaagcggt atcggcacac tgaacctgat
     1561 ggatgcgaca tcgtcgttcg atactgtggg tatcaatgtc ggtaattttg gtagcggtat
     1621 cgtaaatgtc agtaatggtg ccacccttaa ttcaacgggc tatggattta tcggaggaaa
     1681 tgcctccggt aagggaatag ttaatatttc aacggatagt ctctggaatt taaagacgtc
     1741 ttctactaac gcccaattgc tacaggtcgg tgtattaggc acgggtgaac tgaatattac
     1801 caccggaggt atagttaaag cgcgtgatac acagatagct ctcaatgaca aaagtaaggg
     1861 cgacgtgagg gtggatgggc agaactctct tcttgaaaca ttcaatatgt acgtagggac
     1921 atctggtacg ggtacgttaa ccctgacgaa tagcggcacg ctgaatgtcg aaggtggaga
     1981 agtttactta ggtgtttttg aacctgctgt aggaacgcta aacattggtg cagctcacgg
     2041 tgaggcggcg gcagatgccg gatttatcac caacgcgacg aaagtagagt ttggctctgg
     2101 cgaaggtgtt tttgtcttta atcatactaa taacagtgat gccggctacc aggtcgatat
     2161 gctgattaca ggtgacgata aagacggaaa agtgatccat gatgcaggcc atacggtgtt
     2221 caatgcaggg aatacttata gcggtaaaac gctggtcaat gacggcctcc tgaccattgc
     2281 gtctcatacg gcagatgggg taacgggcat ggggtcgagt gaagtaacca ttgcaagccc
     2341 cggtacgctc gacattctcg catcaacgaa cagtgcagga gattacacgc tgaccaatgc
     2401 gctcaaaggc gatggcttga tgcgagttca gctgtcatcc tccgacaaga tgtttggctt
     2461 tacacatgca acagggactg aattcgccgg tgttgcccaa ctgaaagaca gtaccttcac
     2521 tctggaacgc gacaacaccg ctgcgcttac tcacgcgatg ttgcagtctg acattgaaaa
     2581 taccacatcg gtaaacgtgg gagagcaatc cattggtgga ctggccatga atggcggtac
     2641 gctcattttc gatacggata ttcctgctgc gacgcttgca gagggatata tcagcgtcga
     2701 tacgctggtt gtcggcgcga gtgactacac ctggaaaggc cgtaactatc aggtaaacgg
     2761 gacgggcgac gtgcttatcg gcgtgcctaa accgtggaat gatcctatgg cgaataaccc
     2821 tctgacgacg ctcaatttgc tggaacacga tgataaccat gtcggcgttc aactggtgaa
     2881 ggcgcaaacg gttattgggt cgggtggctc attaacgtta cgtgatttac agggcgacga
     2941 ggtggaagcg gacaaaacgt tacacattgc gcaaaacgga acggtggtcg ccgagggtga
     3001 ttatggattc cgcctcacga ccgcaccagg tgatggtttg tacgttaact atgggctgaa
     3061 agcgctgaac atccatggag ggcaaaagct gacgttagcc gaacatggcg gagcctatgg
     3121 cgcaacggcc gatatgtcgg caaaaatcgg tggtgaaggg gatctggcaa tcaatacggt
     3181 gcgacaggtt tcgctttcca acggtcagaa cgactatcag ggggcaacct acgttcagat
     3241 ggggacatta cgtaccgatg cggatggcgc gctgggcaac acccgggaac tgaacatcag
     3301 caacgcagcc atcgtcgatc ttaatggatc gacgcagacg gtagagacat tcaccgggca
     3361 gatgggttcg actgttttgt tcaaagaagg gtcgctgacg gtaaataaag gtgggatcag
     3421 tcagggtgaa ctgacaggtg gcggaaacct gaatgttaca ggggtaaagc agatatcccg
     3481 ctaggcatga ttagagaaga aaatgacttt cgcatctcgg ttgctggcgc acaggagaag
     3541 acagcactgc tcagaatagg caatgactgg tgcattccga aaggaataac gccgacgacg
     3601 cacatcatta aattaccgat tggggaaatc aggcagccca atgcgacgct cgatctcagc
     3661 caaagcgttg ataatgagta ttactgtctg ctgctggcga aagaacttgg gttgaatgtc
     3721 ccggacgcag aaatcattaa agcgggaaat gtgcgcgcgt tagcggtcga acgttttgac
     3781 aggcgttgga atgctgagcg aacggtttta cttcgcttgc cacaggagga tatgtgtcag
     3841 acattcggtt taccttcatc ggtgaaatat gaatcagatg gaggcccagg catcgcgcgg
     3901 atcatggctt ttttgatggg gttcagcgag gcgcttcgcg atcgttatga ttttatgaaa
     3961 ttccaggtct tccagtggtt gattggcgca acggatggcc atgcaaaaaa cttctccgta
     4021 tttattcagg ctggcggcag ttatcgactc acgccatttt acgacatcat ttcagcattt
     4081 ccggtccttg gcggtacggg aatacacatc agcgatctca aactggcaat ggggcttaac
     4141 gcatccaaag gcaaaaaaac ggcaatcgat aaaatttatc cgcgacattt tttggcgaca
     4201 gcaaaggtgc tgagattccc ggaagtgcag atgcatgaaa tcctgagtga ctttgccaga
     4261 atgattccgg cagcactgga taacgtgaag acttcattac cgacagattt tccagagaac
     4321 gtggtgacgg cagttgaaac caatgtgttg aggttgcacg gtcggttaag ccgagaatac
     4381 ggtattaagt aagagtaaga tatggggttt ggtcgttgtt aatgagcagg acaatcatga
     4441 ccgccaacga ctagattctc ccttggttta tattggtgcg cataatgtat attatgttaa
     4501 atcgtatctg agtgatacga agtgattttc cggtagtcgt tattcgttca ggattccaga
     4561 acttaagctt ctcctctttc ataattggcc tgtgcatgac ggatataggc tggctggtat
     4621 gccgatttgc cgtttgccgc atcttcccag cgttgaagac tcaagctggc aaaccgcgtg
     4681 caaatgagca cgccctcttt ccatcaacac ttcaggcagc ttgcgggaat gagtccactc
     4741 cggtatcaga aatggctacg tctcaatgaa gtcaggcgaa cgatgctgaa tgaacattac
     4801 gatgtcacca cagcagccta tgctgtcggt tacgaaagct tatcccattt ccgacgggaa
     4861 tatttgcgga tgtttggaga gtcacctaag agagatatta ccaggttaag aaagtctgtc
     4921 ggtcagcttt aataaaccct gcacttatct gtttagagca attcggtgtt agttacacca
     4981 ggcaaacatt aactatagct aatgatttat ag
//
LOCUS       DAZKFZ010000271         4851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000271 DAZKFZ010000000
VERSION     DAZKFZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(66..827)
                     /gene="kduD"
                     /locus_tag="ACHOQW_004435"
     CDS             complement(66..827)
                     /gene="kduD"
                     /locus_tag="ACHOQW_004435"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP9575379.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(857..1693)
                     /gene="kduI"
                     /locus_tag="ACHOQW_004436"
     CDS             complement(857..1693)
                     /gene="kduI"
                     /locus_tag="ACHOQW_004436"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9575380.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1980..3161)
                     /gene="yqeF"
                     /locus_tag="ACHOQW_004437"
     CDS             complement(1980..3161)
                     /gene="yqeF"
                     /locus_tag="ACHOQW_004437"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP9575381.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3416..4645
                     /gene="yqeG"
                     /locus_tag="ACHOQW_004438"
     CDS             3416..4645
                     /gene="yqeG"
                     /locus_tag="ACHOQW_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9575382.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1267 a         1236 c         1055 g         1293 t
ORIGIN      
        1 tatgatgatc tagagatata aaaaaccctg ccatgcggca gggtcataaa agtaagagga
       61 atgaattaac gcgccagcca accgccatcc acagcaatgg tataaccatt tacataatct
      121 gaagcgctgg aggcaagaaa cactaccggc cccatcaggt cactcggcag tccccaacgg
      181 ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg cagttgttgg
      241 gtattgttgg tcgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc
      301 cattcattcg ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc ggtataagaa
      361 ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca
      421 ttgccttgcg cgatgaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata
      481 ttcaggttca tgacatcgtc ccagtccttt tcgctgaact cgagagcatc ttcgcggcga
      541 atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac cgcgcgatcc
      601 agcagtccag gaataccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc
      661 cccagcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg
      721 tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagcccagt atcacaacca
      781 gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac aataccttat
      841 tcgtttatgc ccacaactag cgcaaatctt taacggccac atggtccata tcatcaaaga
      901 cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat
      961 gaatcgacca gctcggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
     1021 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
     1081 aaacttccat ccggcgctcg tgggtgtgac acggcatggt gttccacaag ttacccggag
     1141 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
     1201 tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
     1261 ccggtgtgac ttttttggta ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
     1321 tcgccggagt gccggtatca atactagcaa agacaacttc ttttgcgcct ttacccacat
     1381 acagggcgtc gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac
     1441 cgccaatatt gataacacct aactcgcgac gttcaaggaa ataacttacg ccgagttgtt
     1501 tacccacttc cccgccaacg gaaaccgttt tagttaccgg cataatgccg ccaataataa
     1561 tacggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
     1621 aaaattcatt gcgcagcccc tgggtttcca gcgtttttgc gtgcgcactg tggatgctct
     1681 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
     1741 aaacactgtt tcgttttctc tataagggga tgatatgccc atgggatcgg gttttcaatt
     1801 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
     1861 gaatctgtca tgagaatgga aaaatcaggg agcccggaaa atgtatgtcg acttgccagc
     1921 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
     1981 tattcgtcac gttcaatggt caatgccaca ccctggcccc cgccgataca aagcgttgcc
     2041 agtcctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
     2101 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
     2161 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
     2221 tcgataagat caacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
     2281 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
     2341 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
     2401 gctgcgccat cgtttatgga tgatgcatta cccgctgtca cagaaccgag actatcaaat
     2461 gaaggattta aacgggctaa accttctgca ctggcgtcag tgtgtggctg ttcatcggta
     2521 tcaacaacca acgtctgacc gttactttgg gtcattaccg ggacgatctc atctttaaat
     2581 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
     2641 aattgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
     2701 tcattgaagg catcccataa cccatcatgc acaagactgt caaccagctg gctattgcca
     2761 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
     2821 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
     2881 gctttaagcc cggaaccgca gacgtcatta atcgtgattg cagaaacgct attaggcaga
     2941 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
     3001 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
     3061 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccgcg aaagcagccg
     3121 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
     3181 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
     3241 tttatcacaa aggaaatata cctgagcagt agtcagagtc ataactggca cgtaaggttt
     3301 gcaaccacta ccccaccaat agaggggtag atagggcgtt aatctcccat acttaacctg
     3361 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
     3421 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
     3481 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
     3541 tactgctctg gtcgcctggc ctttaacata ctggccacat aaagctttat gccagttcat
     3601 cctctcatcg aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg
     3661 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
     3721 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
     3781 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
     3841 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
     3901 ctatttctta tttctttcca tttaccttgt aggtagttgg caacctgatc tattaacaac
     3961 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
     4021 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
     4081 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
     4141 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
     4201 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
     4261 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
     4321 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
     4381 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
     4441 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt atgcgatcag
     4501 cggcccgctc attgccatga tacttttcat catgcctacg ctatcaacat atctcatccc
     4561 ggcgcttaaa ccctggcgct ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
     4621 cgtatcggta atgttcttta gctaatatcc attgtgaatg gttcaaaatt gctacaattt
     4681 tgaaccattc cagttttcat aatgtgcatt tgttattatt ccgctctatc acttctctcg
     4741 tcatatccta gtattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
     4801 actatttaag atgcataact tagattcgca agatatcttc tggtcattca a
//
LOCUS       DAZKFZ010000272         4828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000272 DAZKFZ010000000
VERSION     DAZKFZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..591
                     /locus_tag="ACHOQW_004439"
     CDS             <1..591
                     /locus_tag="ACHOQW_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9575383.1"
                     /translation="DHDELLSTLSYDSETGIFKWLKTNSVVRVKGSIAGGVSGGYICI
                     SINNVLYKAHRLAWFYVYKKWPPKFIDHVNGNRLDNRISNLRLATEEQNARNIVGNRL
                     NTSGAIGVSWYKPTGRWKSYVGYKNKTISLGYFDSKEDAAFIAALARKKLYGTYASKA
                     LNCEHELLSQFNNDEDKLAEYLKEKYKRTRKRVKKR"
     gene            1055..1387
                     /locus_tag="ACHOQW_004440"
     CDS             1055..1387
                     /locus_tag="ACHOQW_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575384.1"
                     /translation="MDESRKQFEEYVAKKLRLPFEMITEARNGDRYFAFSSMDIRHSL
                     NDWWALWQASRAAIELDIDWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAA
                     GIKEKNFR"
     gene            1384..1794
                     /locus_tag="ACHOQW_004441"
     CDS             1384..1794
                     /locus_tag="ACHOQW_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9575385.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            1791..1982
                     /locus_tag="ACHOQW_004442"
     CDS             1791..1982
                     /locus_tag="ACHOQW_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9575386.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKPKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTRGLT"
     gene            2248..2580
                     /locus_tag="ACHOQW_004443"
                     /pseudo
     CDS             2248..2580
                     /locus_tag="ACHOQW_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            2851..3117
                     /locus_tag="ACHOQW_004444"
                     /pseudo
     CDS             2851..3117
                     /locus_tag="ACHOQW_004444"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            3214..3519
                     /locus_tag="ACHOQW_004445"
                     /pseudo
     CDS             3214..3519
                     /locus_tag="ACHOQW_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
     gene            3594..4316
                     /locus_tag="ACHOQW_004446"
     CDS             3594..4316
                     /locus_tag="ACHOQW_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFP9575387.1"
                     /translation="MNELINSNVIKMTSIEISELTGKRHDNVKRTIETLAKNGVIRHP
                     QIEDCGRINGLGLNQSFRVYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKRFAWMRDHKILIASGARRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            4316..>4828
                     /locus_tag="ACHOQW_004447"
     CDS             4316..>4828
                     /locus_tag="ACHOQW_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9575388.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNR"
BASE COUNT         1531 a         1017 c         1156 g         1124 t
ORIGIN      
        1 gatcacgatg agcttctgtc aacattatca tatgactcag aaacaggaat atttaaatgg
       61 ctaaaaacaa attcagtagt aagagtaaaa ggtagtattg ctggaggtgt tagtggtggt
      121 tatatatgca ttagtataaa taatgttttg tataaggcgc atagacttgc ttggttctat
      181 gtatacaaaa aatggcctcc taagtttatt gatcatgtaa atgggaacag acttgataat
      241 aggatttcaa acctaagact ggcaacagaa gagcagaatg caagaaacat tgtagggaat
      301 agattaaaca catccggtgc aattggagtg tcttggtata agccaactgg caggtggaag
      361 tcttatgttg gttataaaaa taagacaata tcgttagggt atttcgatag caaagaagat
      421 gcagcattca tagcagcact agcaagaaag aaactatatg gaacttatgc gagtaaagca
      481 cttaattgcg agcatgagct tttatctcaa tttaataatg atgaggataa acttgcggaa
      541 tatcttaagg aaaaatataa aaggactcga aagcgtgtta aaaaaagata aaggcctgct
      601 ggtaatcgca ggccttttta tttgggggag agggaagtca tgaatctgga caagcaagat
      661 gcacaaacta ttagctcata cataagggca tcaagaccag attacaaagg tccggtattc
      721 gtagatttat ctcgccttga atagatttac atgtggaaag caaggttact tacgcatttt
      781 gttattcgaa cgatgactag caacattaca aaaccaatgt aactggaaga ggtgaatatg
      841 agcacactcg cagaccttat tcatgccgat atggctgaag atggagcaag gcgtaatagg
      901 tactggaaat catcgagcct tccagtttgt gaaagattca atcacaggcc aaaaccaaaa
      961 cgtagccgac gagacaaagt gttgaaaaaa ctcatgcaaa ttaacatggc tggttttgtc
     1021 agattcgtga gtgaaacgac taacggtgat tgatatggac gaatcaagaa agcagtttga
     1081 ggaatacgtt gccaaaaaat tgagattacc attcgagatg ataaccgagg caagaaatgg
     1141 tgataggtac ttcgcatttt caagcatgga tattcgtcac tccttaaatg attggtgggc
     1201 tttatggcag gcatcgcgag cagctattga actggatatc gactggcccg aatcgaatga
     1261 cgacctttgg aaagatggtg aagaaggtgc ttatgcgatg ggttatgagg atgggcgtga
     1321 caaaacggta attgcagtaa tgaaagctat cagagccgct ggaattaaag agaagaattt
     1381 tcgatgaagc aaacaatctt cctccgaact aagcaacaac agcaagctgc aataaatgcc
     1441 atcctcgcaa caccactcga taaagacaag ccagtcacca tccgcattac tgactacaag
     1501 cgcaaccttg accagaacgc aaaatttcac gcgatgctgg cggatatcgc tcgtcaggtt
     1561 caatggtgcg gcaaatggtt aaaaccagaa caatggaagg ttttgttgat cagcggtcat
     1621 gcagtggcaa caaagcagga agctgatgtt ttgcccgggc ttgaaggcga atacgtcaac
     1681 attcgcgaaa gcagcgcgca gatgagtgtg aagcgtatgg caagtctgat tgagtacacg
     1741 acagcatggg ctattggtca gggtgtcaga tttaccgaca ggaggtacga atgagacgac
     1801 agcgacgaag tatcaccgac ataatctgtg aaaactgcaa ataccttcca acgaaacgct
     1861 ccagaaataa acccaagcca atcccaaaag aatctgacgt aaaaaccttc aactacacgg
     1921 ctcacctgtg ggatatccgg tggctaagac atcgtgcgag gaatacaagg gggttgacgt
     1981 gatgatttat ccggggctat attcctcaca cgccagcaaa atctggcgtc gggattggcg
     2041 tcctggatag agaccgcgac agatacacgc cgcgagcgtg ttttttattg tcgtatgcac
     2101 gcgcacatct gaattatggt gggctgtgtg ggggcggaga gatccgcgcc ggtcggtttc
     2161 ccggttacgc caaccctgca cagttcacca ccagacgatt ggcgtcgtcg gtggtgagtt
     2221 attaagaaac cactcgaggg cgtcattatg acaactcaaa tctctgttga aactctctcc
     2281 ccgatcaccc ataaccagat tcctgttatt accaccgaac ttttggcgca gttgtacggt
     2341 gctgaagtta agaacattca aaataactac gccagaaatg ctgagcgctt tattgaagga
     2401 aagcattttt tcaaagtggc tggcgatgcc ctgaaaaatt tgcgggttac tttaagtaac
     2461 tcacaaaatt tgcaaccatc tttaagaggt ttgcaaattt cccccaaaac tcgctccctc
     2521 atcctctgga cagaacgagg cgcagcccgt cacgccaaaa tgctcgaaac cgatcaggct
     2581 tgacgttata ggttgttact tgtagattta tcttcgtgag gcgtcagaac ctctctaaag
     2641 cggacaaacc caaccccgat agtgttggat tttttatgcc tgtcatttta tggacgcatt
     2701 gcgcggtcat accccgaaca atgtcgggag ggcgacgaat acaacacccg aaaggggaat
     2761 gagtccgcgg ttctttaggc cgttctgaac ctcccgacac cacaatcaga agtggtaatt
     2821 cagaaaaagc taaaggaggt cgttatgacc atgcaactcg ccgtagaatc cctcgtcgtt
     2881 gttacttact cgaacattcc cgtaataaca acggagttat tggcaacact atacaaaaca
     2941 gaacaaaagc atatacggca aaatttcaaa cgtaacgaat gtcgttttat tgcaggaaaa
     3001 cacttcttta aagtaagtgg tagtgaactc gatgatttga gaacctctca aagaggtttg
     3061 caaatttccc ccaaaactcg ctccctcatc ctctggacag aacgaggcgc agcccgtcac
     3121 gccaaaatgc tagaaaccga tcaggcgtgg gaagtgttcg aaaaactgga agactgctat
     3181 ttcagccaga cttcgaaaag cgcaggtcat cacgagaaga aaaccagcga gctttctgca
     3241 aaagatgcaa acagtctggt atggttgtgg gattatgcca accgctcaca ggcattgttc
     3301 cgcgaactgt atccggcgct aaaacaaatt caatcgaact attccggcag atgctacgac
     3361 tacggccatg agttttcgta tgttatcgga atggcgagag atgttttaat caatcacaca
     3421 cgagatgttg atattaatga tccagacgga ccaacgaatc tttccgcatg ggtaagactt
     3481 aagaacaaag aattacctcc tgcactgcat cgctactgac aattgacaac ttaacaaacc
     3541 cagcttcggc tgggtttttt attggtgaat tttcaatatg agaggacatg acaatgaacg
     3601 agctgataaa tagcaacgtc atcaaaatga cgagcattga aatctctgag cttacaggta
     3661 agcgtcatga caatgtgaaa cgtactatcg aaacgctggc taaaaatggt gttatccggc
     3721 atcctcaaat tgaggattgt ggaagaatca atgggttagg cttaaatcaa agttttcgtg
     3781 tgtatgtatt cgaaggcgaa caaggtaagc gcgatagcat cattgttgtc gctcagttgt
     3841 cgccggaatt caccgctcgc cttgttgacc gctggcgaga actcgaaggg gcaaccgcga
     3901 aaataccaca aaccttttct gaggcattgc gccttgcggc cgaccttgaa gaccagaagg
     3961 ctgaactgga gaaacagctt gctctcgcag cacctaaagt tgagtttgcc gatcgagttg
     4021 gcgaggccag cggaattttg attggaaact ttgcaaaggt tgttggtatt ggtccaaaca
     4081 aacggtttgc gtggatgcgc gatcacaaaa tccttattgc ttcaggtgcc cggcgcaatg
     4141 tgccaatgca ggaatatatg gatcgcggct atttcacagt gaaagaaaca gcggtcaata
     4201 caaatcacgg aatacagata tcgttcacca caaaaatcac cgggcgtggt caacagtggc
     4261 tgacaagaaa gctgctagat aacggaatgc ttaaagtaac aggggaggct gcttaatggc
     4321 taaaccagcg cgaaggaaat gcaaaatctg taaggaatgg tttcacccgg cattctcaaa
     4381 tcagtggtgg tgcagcccgg aacacggaac taaattagca ctcgaacgac gaaataaaga
     4441 acgcgaaaag gcggaaaaaa cagcagagaa gaaacgacga cgagaggagc agaaacagaa
     4501 agataaaatt aagattcgaa aactcgcctt aaagccccgc agttactgga ttaaacaagc
     4561 ccaacaagcc gtaaacgcct tcatcagaga aagagaccgc gacttaccat gtatctcgtg
     4621 cggaacgctc acgtctgctc agtgggatgc cggacattac cggacaactg ctgcggcacc
     4681 tcaactccga tttgatgaac gcaatattca caagcaatgc gtggtgtgca accagcacaa
     4741 aagcggaaat ctcgttccgt atcgcgtcga actgattaat cgcatcgggc aggaagcagt
     4801 agacgaaatc gaatcaaacc ataaccgc
//
LOCUS       DAZKFZ010000273         4804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000273 DAZKFZ010000000
VERSION     DAZKFZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            158..724
                     /gene="slp"
                     /locus_tag="ACHOQW_004448"
     CDS             158..724
                     /gene="slp"
                     /locus_tag="ACHOQW_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP9575389.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            880..1410
                     /gene="dctR"
                     /locus_tag="ACHOQW_004449"
     CDS             880..1410
                     /gene="dctR"
                     /locus_tag="ACHOQW_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9575390.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1471..2499)
                     /gene="chuS"
                     /locus_tag="ACHOQW_004450"
     CDS             complement(1471..2499)
                     /gene="chuS"
                     /locus_tag="ACHOQW_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFP9575391.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTHQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(2548..4530)
                     /gene="chuA"
                     /locus_tag="ACHOQW_004451"
                     /gene_synonym="shuA"
     CDS             complement(2548..4530)
                     /gene="chuA"
                     /locus_tag="ACHOQW_004451"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFP9575392.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1279 a         1171 c         1086 g         1268 t
ORIGIN      
        1 tcatgccacg ccccaaaata acggaattga actaaacaga agggatataa acatcagaca
       61 ggtttacgtt actatcaggc atatcgcctc agaatcagat gaaaactata aagaaatatc
      121 tattatggtt ttaatatttg ttgataagga tagtaacatg aacatgacaa aaggtgcact
      181 catactcagc ctttcatttt tgcttgcagc atgtagttca attccgcaaa atatcaaagg
      241 aaataaccaa cctgatattc aaaaaagttt tgttgctgtt cataaccagc cggggttata
      301 tgttggtcaa caagcgcgct ttggtgggaa ggttatcaac gttatcaatg gcaaaacgga
      361 tacgttgtta gaaatcgctg tattaccgtt ggatagctat gcgaagcctg atattgaagc
      421 caactatcag ggccgactgc tcgccagaca aagcggcttc cttgatccag tgaactatcg
      481 taatcacttt gttaccatcc tcggcaccat tcagggtgaa caacctggct ttatcaataa
      541 agtcccgtat aacttcctgg aagtgaatat gcagggcatc caggtgtggc atttgagaga
      601 agtggttaat accacctata acctgtggga ttacggctat ggtgcattct ggccggaacc
      661 gggctggggt gcgccttact acaccaatgc ggtgagtcag gtaacacctg agctggtcaa
      721 ataacaccac cgaaagatgc agatgctttc tcagcatctg catcatgcat tacatcaaat
      781 taatacacag taagctaact attattatta taagccctgt cctgttaatt acctttggca
      841 aactgattat aaagttaatg tccgcaccag gagtcggtta tgtttcttat aattaccagg
      901 gatacgatgt tcttcaccgc gatgaaaaac attctgagta aaggtaatgt cgttcatata
      961 cagaacgaag aagagatcga cgtaatgttg catcagaatg ccttcgtcat tattgataca
     1021 ttaatgaata atgtatttca ttctaatttt ctcactcaaa ttgaacgatt aaaacctgtc
     1081 catgtcatta ttttctcccc ctttaatatt aaacgctgcc tggggaaagt gccggtgacc
     1141 tttgttccgc ggactatcac tatcattgat tttgtcgcac tcatcaatgg cagttactgc
     1201 tctgtgcctg aagcgaatgt gtcactttcg cgcaagcaac atcaggttct gagctgcatt
     1261 gcgaatcaaa tgacaacgga agatattctg gagaaactga aaatatcgct aaaaacgttc
     1321 tactgccata aacacaatat catgatgatc ctcaatctta agcggatcaa tgagctggta
     1381 cgccatcagc atattgatta tctggtgtga acgattgatc aatataaaga ggcccagcaa
     1441 taaatagcca gacctcccgt taattatacg ttatgcagca acgccttccg gtatcagcga
     1501 agcaatttgc ttacgccatt gcgcttgctc ctgttcgcct tctgtacgtt gaccataaag
     1561 ttgcgctatc tgcgtaccat catgggcaaa caattccaga ctggttacgt agccatcgct
     1621 ggtcggttta cgggtaaccc aggcttcagc aatgctctct tctaatagat gaagagtaaa
     1681 cgtcgggttg aaaatattca gccaaccttt cattggcacc actttttcta ccacaccggt
     1741 gaaaatctgt acgcagccac ggttgccaac aaacaccatg atttcattac catcctgctg
     1801 tgcagattca agaatttgcg ccaacgcact gttggatact ttgcaggcca aatcgtctgc
     1861 caccagattg aacgcctgtt ggcgcgtcag gttgtggcgc ttgagcaacg taaaaaactg
     1921 atgaacgtcg gtcatcgccc gccactcttg ctcgaccaca gtggcatcgg ctcgcgtttg
     1981 aacaactggc gcatcaacgg cttttaactc aagcggcgta ttctcatcgg tgataaaccg
     2041 tgccagaagc tcactccatg ccgccatatc ggtattgtcg gtggcataaa cttttagtaa
     2101 tgcatcgccc tgatgatcaa agaactgaat actctggcgt tcgccacgag ccgtgttttc
     2161 tttgatgtgg aaaacactgg cccattgatt gagaaacaga cgtaaatcca gcgcgcgcgg
     2221 attgaggatc aatccggcat gtccgttcaa atgctggtgt gtgaacgtac caacttgctc
     2281 atggactgca tattcattac gacaaataca tttggtttcg ccaacacttt cgagcgccgc
     2341 cagaatttca cggatatcgc cgtgcatccg ccacgcatcg tgcgtgacgc gtgcaaatgc
     2401 cagttctgct tctctaatat tcattaaccc tgcgatgtca cgcgcgtact ttccgggatt
     2461 ttgttctttt aactcaagcc agcgtgtgta gtggttcatt gtctctcttc cttccagata
     2521 aatgccccga aatatcgggg cagatgatta ccattgataa ctcacgaaaa tttttccgtt
     2581 acgaccatcc tgtgggatgc cttgcggcga ccagtactct ttgtcgaaag cgttacccaa
     2641 caccaaagta gtggtcatac ctttgagcgc ctgttgtcct tgataactga cgtagaaatc
     2701 attcacgcca tagcctggtt gtttgctgta actgctgctg atatgtgttg agcgatcggc
     2761 aaacgtacca acccacccaa cagagaagcc actgtgagcg atcggaatat tcagagtgct
     2821 ggtaacagta tccgggttaa tgctggagat gtattcgccg gtatcggtgt ctttgccgcg
     2881 ggtacggtta taggccacat caaggctaaa cagatcagtg gtatatttcg tcatcacatc
     2941 ccagccccag attttggcgt tcgggacgtt atacgacata gtcgtcgccg ccgcgaaatc
     3001 gacggtcgtg gagatgtaat ccttcgcttt ggtatcaaag tagctggctt taaattccag
     3061 agcatcattg gacaacatca ggtcatcaaa acgcagccca aaaccgtact cctgagtttc
     3121 gttagtttcc ggacgtaagt tcgggtttgg cacccaatag ttggtataga agcgaccaat
     3181 cgagaagtgc ttagaatcgt tatacatttc gcccatcgtc ggggcgcgga atgcctgggc
     3241 atatgagcca aataacatca gccagttagt cggattgata gtcatccccg cacgagatga
     3301 ccatttgtcg gcatcaacat ctttgtaacc gtcactgcta ccgcgataac tgtcatagcg
     3361 ggttccgcca agcagggtaa tcggcagatc gcgtaaggtg atctcatcct gtagccagcc
     3421 ggagctaaaa tcgatttttg cttgcgggaa gcccgtcgtc gcgccgcccg gatgttgttc
     3481 ctgacgataa tactcaccgc catatgtcag taagtgagaa gcgaaactgt cggcaaagag
     3541 agtggaacgg ttctccagcc tggctccttt tgttatctgt tcacgatact cgccggaact
     3601 ccccgtgttt tgcgcattaa tacggacttc cgaccaataa atttttgcat ctgcatttaa
     3661 ccagtcgttg ccctgcgggg cgagtttata agaaagctgc gcatcgcgtt gaattgttga
     3721 acgatcaacc atcgggttgc tgctttcaga agcctcaacg gtctgcggat tttttggttc
     3781 acgcgcgtcg ttgttgtagt aacgcactaa accgctcaga gactgggctg aatcaatttg
     3841 ccaggtccct ttcgccagca tgttattaat ggactcgtca ttcggcgcgg tttcaccatt
     3901 gctctggcgt aaatcacccc gatcgcgact ggaccaggcc acaataccat ccagattttc
     3961 agttcgccca aacgcgctcg cgcctaatcc caggctatgg tcccccgtgc cgccagtacc
     4021 aaagacacga aaaccactgc tttgtccttc ctgcaataaa tcttttgcat cgaccgtatc
     4081 gtaggagatc actccaccca gcgcgccact gccatacagt aatgctgaag gtccacgaac
     4141 aatctcaaca cgcttgatca gcgccggatc gagaaaagtg ccattcaggt gtccggtatc
     4201 cgttccctga cgaacaccat cgacaagaac cagcacgccg cgatgatcat agccacgcat
     4261 atttacatcc tgaccgttgg ttcgtccggt accatccaga gtaattccag gaacatgacg
     4321 cagcagatcg gtggctgaag tagccgtttg attttcagga gcggaagtgt cgatgacgct
     4381 gaccatcata ggcgcttcga aggaactacg ggcattcccc gttgccgtaa cggtcatggt
     4441 ttcagtagca aaagcaaacg ttggcaaggt ggcagaaaca gctaaggcca ataaactcaa
     4501 acgcaacgag gtaaattgcg gacgtgacat agcgattctc catgaggaaa ataaaaaatg
     4561 ctggctgcct gggttaatac cggggtggct ggcgaaaaat aaatttattt aaaattcaga
     4621 gcgataatat tcgccgggtg agtttacaag cgtttatatc ccatgaaatt gcctgataac
     4681 tcggcaatta cgcaaaagca cgccctcttt gtcaggcaaa acgatatgcc ccatcaggag
     4741 tgaagagagc tgtgcgttat cccttgcaaa tgctatggtt ttgttatcca tatggtcgtg
     4801 tttt
//
LOCUS       DAZKFZ010000274         4723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000274 DAZKFZ010000000
VERSION     DAZKFZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1004
                     /locus_tag="ACHOQW_004452"
     CDS             <1..1004
                     /locus_tag="ACHOQW_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFP9575393.1"
                     /translation="LFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLSEKK
                     DRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHPSIRRKIAS
                     LSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMV
                     CYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDM
                     EQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNS
                     EAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(1073..2029)
                     /gene="yjiA"
                     /locus_tag="ACHOQW_004453"
     CDS             complement(1073..2029)
                     /gene="yjiA"
                     /locus_tag="ACHOQW_004453"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9575394.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(2040..2243)
                     /locus_tag="ACHOQW_004454"
     CDS             complement(2040..2243)
                     /locus_tag="ACHOQW_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9575395.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2293..4443)
                     /gene="btsT"
                     /locus_tag="ACHOQW_004455"
     CDS             complement(2293..4443)
                     /gene="btsT"
                     /locus_tag="ACHOQW_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP9575396.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
BASE COUNT         1256 a         1147 c         1205 g         1115 t
ORIGIN      
        1 cactgtttga atcagcttct acacataata aaaaatcaga gcaaatagaa tcagcatggt
       61 cgcaaattga agatatggtc ggagtgagac ggcttgataa atttcttact cttcataagc
      121 tttccgagaa aaaagatcgt gacagggtgt tacaaaaagg ttttgaagct tttattgaaa
      181 atctccagca gcaatttgat ggagatgcta ttgctatgtc actgatgttg gttaattctg
      241 caaaaaatta tactaagatt ttagagaatg attttgaaca cccttcaata cgacgaaaaa
      301 tagcatcgct aagtaattta ggggtagatg agtggattcc tccggttatg gcattcatga
      361 atcgaatggc tcgtacagaa gattttaatt tagatgattt ttctcaattt attaccgcct
      421 ttgagaaggt atatatgcat gggtggttaa aaaaacaaat taaaagccaa agagaaatgg
      481 tttgttattc agctctggta gcaataaata atgatatgcc ttttgattct gtgataaatc
      541 agataaacca acatgcagat aattcaggtt ttatagctgc tctggatgaa gatttatatg
      601 agcctcgtcc gaatcaagtg aacttaataa aagcaatttt gcttcgctta gatatggagc
      661 aacaagatga gagcgtgatt aaaacctata cggggagaat cacaattgag catattcttc
      721 ctcaagcatt agttaatgaa tactggatta atagattcca acctcaagag catgtctact
      781 ggctgcataa aataggtaat ttaacactca taagtggttc caaaaattct gaagcacaac
      841 actatgattt tatcaaaaag aaatctattt atgagaaact taatagtaaa agctcttttg
      901 atttaacaaa agatgtttgt aattcttcag aatggggatt ggctgaactg aaaatgagac
      961 atgaaaaaat gaaaacgcaa ttgaagaagc tttggttggt ctgacatatc taaagagttc
     1021 cactcgtaat aatgttgcgg tggaactctc aaaaagttga tctttcccga acttacttcc
     1081 tcaaccccgc aaacgctgcc cgaatctctt cctccggcaa ctgaatcccg ataaaaacca
     1141 ttgtgctatg cggtttttca tcgccccacg ggcggtccca gtcggcgctg tagaggcgct
     1201 ggacgccctg gaacagcagg cggttaggtt cgccgtcaat ccacagcatc cctttgtaac
     1261 gcagcagttt atccgccgat tccagcagca ggttttccat cacgcgggaa acttcgctga
     1321 tatctaccgg gtaatccagt tctaccacaa tcgacgaaat atcgttttgt ttatccgcga
     1381 taaagtggaa acgcggtttg gtgctgacga cgttttcttc cagcataaaa ccgttggtgt
     1441 tgaacagcag gcccaggtcg atgtcgccgt gggtaacggt gtagaccggt gcacgggcgt
     1501 tgatgcgcgc caggcgttcg cgcagttttt ctgcttcgcc tgcgacgtcg gttttggtca
     1561 gcagaatacg gtcggcgtag ccaacctggg actgggcgat ggtgaactgg ttcatctgct
     1621 catcggcatg taccgcatcc accagcgcaa tcacgccgtc cagcaggtaa cgctggcata
     1681 aaatctcatg ggagaaaaag gtctgaataa tcgggccggg atcggccatg ccggtgcatt
     1741 caatgaccag acgatcgaac tgaatattgc ccttatcgag attatccagc aggtcgagta
     1801 gtgcgtcttc cagctcgttg gagcgcgaac agcagatgca gccgttggtc agcgttttga
     1861 tctgcgtggc gcggtcgcca atcagttgat catcaacgga gacttcgcca aattcgtttt
     1921 caatcacggc aatcttgtag ccgtgttgtt cgttaagaat atggcgcagc agggtggttt
     1981 tgcccgcgcc taaaaaaccg gtgagtaggg taactgcaat cgggttcatg ctctctccat
     2041 taacaacagc gcataccgcc tttaccatcg ccgccgtagc gcgcgttctg gcgttcgcgg
     2101 aagaattctt catagctcat gtacggcttg tcgggatggt tggtcttcat atgctcgacg
     2161 tagttgtcgt agtctggaat gccaatcaac atcttcgccg cctggccgag atattttttt
     2221 gcctgtccta agttaccaaa catagttcac tctgataaga acaaagcccc gccgaagcgg
     2281 ggctaaacac ggttagtggt gcgaagagat cttcacgccg ccttccggga ttggaacgta
     2341 cggtgtttct ttgtcagtac gtttgtcgct gttacgtacc gcaagccagg ttttgaaacc
     2401 gtagaagatg atgctgtaca ccacaatcag gaacagaata ctcaggcctg cgttggtgta
     2461 gttgttcaca acgatgtggt tcatgttggc aatctgctgc gccgtaaggt cagtaccgtt
     2521 agcaatcttc tctttgtact ggcttgccat gtagaagaag ccttccatct gcgggttggt
     2581 gctgaacagt ttcagaccca gcgcccaggt ggtgcagata agcagccata cagccggaac
     2641 aacagttacc cagatgtatt gggtgcgctt catcttaatc agcacaacgg tgcccagtac
     2701 cagcgctacg gctgccagca tctggttgga gataccgaac agcggccaca ggctcttaac
     2761 gccgcccagc ggatcgacca cgccctgata cagcaggtag ccccacagac ccacacagcc
     2821 cgcagtaccg atgataccag caaccagaga gtcggttttt ttcaggaacg ggatgaagtt
     2881 acccagcagg tcttgcagca taaagcggcc agaacgggta cccgcatcca gcgcggtcag
     2941 gatgaacagg gcttcgaaca gaataccgaa gtgataccag aagcccatgt cagccatcgg
     3001 cagcactttg tggaacacat gagcgatacc taccgccagc gtcggcgcac cacctgcacg
     3061 gttcaggaca gaaggctcac caatgtcttt cgcggtttgc aggatctgct ctggcgaaat
     3121 cacgaagccc caagagctga cggtcgctgc cgcgtgtgcg gtaacgtctt tcagctgcgc
     3181 catgatgatc ggcgcgttct cgccacccat ttcatgcagg ttaggcatgg tgatgccaag
     3241 gcccgcgggc ggggtgttca tcgcgaagta aagacccggt tcgatgatgg atgcagcaac
     3301 cagcgccata atcgccacga aggactccat cagcattgcg ccgtagccga tgaaacgcgc
     3361 gtcggtttcg ttagccagca gtttcggcgt cgtaccggaa gagatcagcg cgtggaagcc
     3421 agataccgca ccacaggcga tggtgatgaa caggaacggg aacagagcgc ctttccacag
     3481 cgggccagta ccgtcaatgt actgggtcat cgcaggcatt ttcagttccg ggttcagcac
     3541 cacgataccc agcgccaggc cgacgataac gccgatcttc aggaaggttg ccagatagtc
     3601 gcgtggtgcg aggatcagcc acaccggcag cagcgcggaa acaaacgcat agccaatcag
     3661 cgcgaaggta atggtggtgt ctttaaaggt cagtgccgga ccccagtacg gatcgtgagc
     3721 aatcacgcca ccgaagtaga tagaggcaac cagcagcacg ataccaataa cagagacttc
     3781 acccacacgc cccggacgga taaagcgcat gtagataccc ataaacagcg caattggtac
     3841 ggttgagcaa acggtgaaga caccccacgg actttctgcc agggctttaa ccacaatcag
     3901 cgccaggacg gcgaggatga tgatcatgat taagaaacag ccaaacagcg cgatagtccc
     3961 cggtactggc cccatctctt ctttgatcat ctcaccgaga gatgcgccgt tacggcgaga
     4021 ggagataaac agcaccataa agtcctgaac cgcaccggcc agcactaccc ccgccagcag
     4081 ccacagcgtg ccaggcaggt agcccatctg cgcggcgaga accggtccca ccagcggacc
     4141 agcaccggcg atagcggcga agtggtgacc aaacaacacg taacggttgg tcggaacgta
     4201 gttcagaccg tcgttgttaa taaccgcagg cgtcgcgcgc gtggggtcga gtttcatcac
     4261 cttctgggcg atgtacagac tgtagtagcg atacgccacc agatacaccg acacagaggc
     4321 gaccacgatc cacagggcgc tgacgtgctc cccccgacgt aatgcaacta ccgcgaggca
     4381 gaatgcaccg atgattccga gaatcaccca gggtatgtgc ttgaatagtt ttttcgtatc
     4441 catagtaaaa cctggcatgt attgattaat agttggccga agccgttcta ggtttgcgtt
     4501 gcgtttgagg aggtaaattg accgctatcc tgcaatgatg ctgccagagt tacgcgcgcg
     4561 taaagtaagg taaataactg agtggttatt ttagggacgt aagcggtcag ttttgcggtt
     4621 gagcggtttt gagcaaggtt tatgaaggtt atttatgtga tgatgatcac aaccgaagcg
     4681 atttttagcc ctcggttgtg taactcattt aaaaattata ttc
//
LOCUS       DAZKFZ010000275         4720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000275 DAZKFZ010000000
VERSION     DAZKFZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(36..887)
                     /locus_tag="ACHOQW_004456"
     CDS             complement(36..887)
                     /locus_tag="ACHOQW_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP9575397.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(961..1203)
                     /locus_tag="ACHOQW_004457"
                     /pseudo
     CDS             complement(961..1203)
                     /locus_tag="ACHOQW_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(1428..2531)
                     /gene="zorE"
                     /locus_tag="ACHOQW_004458"
     CDS             complement(1428..2531)
                     /gene="zorE"
                     /locus_tag="ACHOQW_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFP9575398.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(2535..3242)
                     /gene="zorB2"
                     /locus_tag="ACHOQW_004459"
     CDS             complement(2535..3242)
                     /gene="zorB2"
                     /locus_tag="ACHOQW_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFP9575399.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(3249..>4720)
                     /gene="zorA2"
                     /locus_tag="ACHOQW_004460"
     CDS             complement(3249..>4720)
                     /gene="zorA2"
                     /locus_tag="ACHOQW_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFP9575400.1"
                     /translation="FIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFAILF
                     NGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLIAQI
                     KLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDFNQN
                     LTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIWEEC
                     KEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGELLKS
                     GAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNSSET
                     LTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQLSS
                     NADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQSLG
                     NALLSISKGFVGNYEKLIKDYQIVMGQLQALISANKHRG"
BASE COUNT         1290 a          998 c          905 g         1527 t
ORIGIN      
        1 agagaatggc tataaattat tagtaaacag aatgtctatc tctcacccac aaacccctca
       61 taagggtaat ttttacgcac ctgcggcaac gcaatagttt tcccgtcaaa atcccagaat
      121 aatctctcaa cgatccctcc gccgtttacc gcatccgaga ccagtacccg catattttca
      181 tccaggccaa tcgatccttt atcaaaggct ttatggtgta ttgcgcaaag cgccagacca
      241 ttggggattt cacaagggcc gccgtgctgt ttccatttta tatgcgccgc ttccagcgcg
      301 acggtggtgt catcgtggcg catattaaag ccacatattg cgcactggta gttataagcg
      361 cgtaatacat ttttgcgaaa taacgggtcg cgctgtttac gggtttgctg gagatcaaaa
      421 cccagctcgt cagcgatttc ttcctgaata ctctcggtga agtgcgcttc gagtatctgt
      481 tgcgccagag tattgatgag ttttttattg ccggttacca gggcgtagtg ctgttcatcg
      541 aaaccgcctg caacgtggta ttcgttcagt tccttcactg gcggttgtcg gctgctccct
      601 gctgttgagc aacgctcggc attgtgtaat tgccagattc cgtcgccttg taatcgccag
      661 aacggcatat caggtcggta ctgtgaacgc tgtggcccaa aacgttccag taaactgtgc
      721 agaggttcgt agatttccgt gccataatcg aaaaggcgcg gatgtccatt caggtatccc
      781 gctaatacgt atagcagtaa caatggttta tgcggcgcgc gctgttcacc cttgtgccag
      841 atttttatgt tggcaattgc ctgctgtagc gatttactgg aagccattga tatcccaggc
      901 agtgaaataa ttgcaatcat gctcgcaaat tttctgctgt tcaagcctgc aagtgaagat
      961 ccattgctgt gttgagggat caaaagacaa caaaaactta ttgaagaaat aatcttctgc
     1021 ttttttgtcg aaataatggg cattgatctt gtctatagct gctgaatctt ttccagcctc
     1081 gttgagatct tcaaaatagc cattgaagat gttctggtta acaccgccag taacataagc
     1141 cttgccgttg tgtacaaaag tcacattacc cgccatgccc atcggcgcgt gcgacatcaa
     1201 tttattgctt tattcattgt gactgtcttc tgtctaccaa gctttaacct tcacccatat
     1261 gagggaagtt ttaagtatga ggcgaaccat agcatatgct tttatacctg ttacaccata
     1321 aaaatcagac aagttatgcc ataaactact tttgtaattt gagccacaaa tcagggaaat
     1381 tttatttatc ttccgtgaaa gtcttaactt tcacggagtt ttagaattta caattttgct
     1441 ggcgtaaagg ctcctgtttg tatttcttta accagctcac ccacggtata tattctccat
     1501 ccttcgactt cggcgggagt aaggttaact gcaattttaa ggcctggcca tgcagcttct
     1561 acggttgcaa taatttcatg ttctgagttt ggaagatcta acccaggaac aggaggttga
     1621 aagcctttat gttgcatcat attaatatca ccatgcactg atggatcagt caggtcatat
     1681 atttcattcc aggattctgc ttttaataac ccttgttggg aacgtaaacg ttgaatgata
     1741 gccatctctg cttgggtaac gaatatacct tcgtggaggg gctgttttcg atggcaatcc
     1801 ttgcacagaa caagaaggtt ttcatggtga ttatcatatt tgatgccatt tttatgatgg
     1861 acatggcaca agtttttggc ggttgataaa ttcacgccac aatcattaca aacataattt
     1921 gccttttctc gtatttcttt agatatttcc ttccagtttt cgacataccc actatttgtt
     1981 ttgtcataga tacccttagg catatgacga aaacaagtac tataatctga aaaaaactca
     2041 tttaatgaga atgatttaaa aatctctctt tttcgttgtt tatcaatact tcctttataa
     2101 ttaagtcgtt cgaggcaatt catacatacc cgtaaatcaa catcctggct ccgacctgaa
     2161 ccatcatcaa tttcgaataa accatctatg cggttagttg catgatacct ttcaaatcga
     2221 tttttatggc gcatttcatc aagagttctg caccaggcaa tatgaaaacg ttttcctttt
     2281 tcgccatcta cgatagccgc atcataacgc cctgaatggt cccgaatata taaaagaacc
     2341 tggcggccat gataagagat aagcccgcta ccagggtcaa gatcttcgat actaacttct
     2401 ttacccgtgg ataactcaat atctatagga tcaaagtcaa taggggattc atccagaaaa
     2461 aaatcgactc cttctggtaa cattttcttc cctaattgaa taagttctga aatgtcgata
     2521 gataatttca tctcttactc ctgaataatc tttctaatct gcaactcggc atttgttaca
     2581 actttgaagg tgacacgacg agagcgatta ggatcctcct tgccggtttt atcaagaatg
     2641 ggatgtgcag atgaataacc tactgcggca aatttacttt taacccattg ttggtgtgtc
     2701 gcgatatttt ttatgtcata aacgtattgt aatactgcac gtgtacgacc ttgcgatagt
     2761 gccatattat taaaataagc aatatcagga ttcgttgttc ctgtccagtc agtacttgtg
     2821 tgaccttcaa tgcggacttc agtaatatgt tcctgataat tatctagaac ttttaggtag
     2881 cgaggaaaga agtcgtcaag aatgagttta aactttggtt ttagctctgt gcttcctaag
     2941 ccaaataaaa catccggtga tttaaatcga acctccagag tctgtttatc gatctctgca
     3001 tcccagtctt gtaaatcctt tgcaaactca atatccagag cattataaag ctgtaactga
     3061 gcattctcgt aggctacggc aacttcttta attttttctt tttcaatacg tacgtagtgc
     3121 atataagcaa tagatatgaa cataaaaacc atcatcagcc cagccattag gtccgacatg
     3181 gatacccaat gttcattatc ttgatctttt ttaggtaatt gtttccctat aatcttatcc
     3241 atgatcgatt acccgcgatg tttattagca gaaattaatg cttgtaactg ccccataact
     3301 atttggtaat ctttaataag tttttcatag ttaccgacaa aacctttgct gattgaaagc
     3361 agagcattac ctaatgattg catttcacgg ttcatttcac gttcagttgc ttgctcaaaa
     3421 gcatgtaact gctcgttgat ggatagagca aatttttcaa ttgattctct gacttgagaa
     3481 gtcagccctg cacttgcctt atcaaatgat gtctggattg aatcaaacat attatcggca
     3541 ttactggata gttgatggtt aatcttttca ccagtcttac tgaacatatc aatcacattc
     3601 gtcgtatttt gttccatatt tctatgcaat tctttcgagt gcgactctag tgatttcagg
     3661 aattcttcac cactttgttt catttcagta attgtttcac ctaacgtgct ggttagggtt
     3721 tcggaggaat tgctgacatc atgtgccatc gaggcaaatg cttgttgtgt ttgggtaacc
     3781 gattgtctga atccttcggt accttcatcc agggcaacgc gcattgaatc tgcattaaga
     3841 agtatttgct ggctggtttg ctcaagagat gcactaacat ttgcagctcc ggatttcagc
     3901 agttcaccca cttcagccat tttgttctgt atttcaggta atacggttgt agctttatcg
     3961 cggatggcga caaaggtttc taaatggcgg gagagttcgc tgatttgatg ttggttcacc
     4021 tgaagcactt cacgcagttc agacatagcc agaggaattt ctttacattc ttcccaaatc
     4081 cctgcaaccg cagtttttgt ttcaaccagg gactcgacac tttgttgata ttgttctgac
     4141 atctgctcaa tttgcgtttt ataatttccc tgccactcaa caagtttttt tacagaggca
     4201 tcaagagctt taaagttttc accaaactgt tcagttaaat tttgattaaa atcaataatg
     4261 acttgtcgca aagcatcaat aatttgttct gtagcacctt ttgccattag atctgcaaat
     4321 tgttcaagtt gttcccaaag cttattactg aaatgagtgt gattagctaa ttgtgcctgc
     4381 gaggaatcgc taatctcagt acgtagtaat tttatttgag caataagaga accttcacta
     4441 tcaccgttaa tacccgagac taatttagtc agagtctggt tctgctcttt taattcatga
     4501 tagatatgtt caggtgtaac agattcaggg ttattttctg ctaacgcact tcgtttattg
     4561 gcaaaaaaga aagcatccat tccattaaat aaaatggcaa aaaacatacc tacaatgctt
     4621 gtaatgaatg ctgtttttag gccacctaat aatacgggga tgctggtatc aatactttcg
     4681 gtattaaaat tgagtaaacc aataataata ccaatgaaag
//
LOCUS       DAZKFZ010000276         4716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000276 DAZKFZ010000000
VERSION     DAZKFZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.277"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            84..626
                     /locus_tag="ACHOQW_004461"
     CDS             84..626
                     /locus_tag="ACHOQW_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP9575401.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            726..1928
                     /locus_tag="ACHOQW_004462"
     CDS             726..1928
                     /locus_tag="ACHOQW_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP9575402.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2148..2930
                     /locus_tag="ACHOQW_004463"
     CDS             2148..2930
                     /locus_tag="ACHOQW_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9575403.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2945..4145
                     /gene="rhmD"
                     /locus_tag="ACHOQW_004464"
                     /pseudo
     CDS             2945..4145
                     /gene="rhmD"
                     /locus_tag="ACHOQW_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            4202..>4716
                     /locus_tag="ACHOQW_004465"
     CDS             4202..>4716
                     /locus_tag="ACHOQW_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9575404.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMG"
BASE COUNT         1077 a         1163 c         1295 g         1181 t
ORIGIN      
        1 ttcgctcatt gaatcctctg cgccaggcag tacaatcccg cagctttggg aattttgatt
       61 aattgcttgt gggaaagggg ataatgaaaa aaattgcgct agcaggtctg gccggaatgc
      121 tgttggtttc tgcatcggtc aatgcaatga gcatcagcgg ccaggcgggt aaagaataca
      181 ccaatattgg tgtcggtttt gggactgaat cgacgggcct ggctttaagc ggtaactgga
      241 cacataacga cgacgacggt gacgtcgcgg gcgtggggct ggggttgaat ctgcctctcg
      301 ggccgttaat ggcgaccgtt ggcggaaaag gcgtttacac caacccgaat tacggcgatg
      361 aaggctatgc cgcagcggta ggaggtggtt tgcagtggaa aattggcaac agcttccgtt
      421 tgtttggcga gtattactac tctccggatt cactctccag cggtattaaa agttatgagg
      481 aagcgaatgc tggcgcgcgt tacaccatta tgcgtccagt cagtattgag gcgggttatc
      541 gctacctgaa tctgtcgggt aaagacggta accgcgacaa cgccgtggct gacggcccgt
      601 atgttggggt taacgccagt ttctgatccc ttccggcgcg gcattctgtc gcgccattca
      661 gtttcttact acgccttatc tccctttgcg ttatagtgat tcgataacaa aagcaggaga
      721 taaccatgtt aaaagtggaa atgctatcca ccggggatga agtgttacac gggcaaatcg
      781 ttgacactaa cgctgcctgg ctggccgatt ttttctttca tcaggggttg ccattatctc
      841 gccgcaatac ggtgggggat aaccttgatg acttagtcac cattcttcgc gaacgtagtc
      901 agcacgccga tgtgctgatc gttaacggcg ggctgggacc gaccagcgat gatttaagcg
      961 cactcgccgc tgcgacagca aaaggtgaag gcctggtgct gcatgaagcc tggctcaaag
     1021 agatggaacg ctatttccac gaacgtggac gagtaatggc accgagcaac cgtaaacaag
     1081 cggagctgcc tgccagtgct gaatttatca ataacccggt aggcaccgcc tgtggttttg
     1141 ccgtgcagct taatcgttgc ctgatgttct ttactcccgg cgtaccgtca gaatttaagg
     1201 tgatggtcga gcacgaaatc ctgccgcgtc tgcgcgagcg tttttcttta ccacagccgc
     1261 cggtttgtct gcgtttaact acctttggtc gttcggaaag cgatctggca caaagcctgg
     1321 acactctaca actgccgccg ggcgtaacaa tgggctatcg ctcctcaatg ccgatcatcg
     1381 aactgaaact caccggaccg gcaagcgagc aacaggcgat ggaaaaactg tggctggacg
     1441 ttaaacgtgt tgccggacag agcgtgattt tcgaaggcac cgaaggactg cccgcgcaga
     1501 tcagtcgcga attgcaaaac cgccagttca gcctgacgtt gagcgagcaa ttcaccggtg
     1561 gtttattggc tttgcaactt tctcgcgcag gtgctccact gctggcgtgt gaagtggttc
     1621 cttcacagga agaaaccctg gcgcaaactg cgcactggat tacagaacgg cgagccaacc
     1681 attttgccgg gctggcactg gctgtttcgg gtttcgagaa cgagcatctc aactttgcgc
     1741 tagccacgcc agatggcact ttagctctgc gtgtgcgttt cagcactaca cgctatagcc
     1801 tggctatccg tcaggaagtg tgcgcaatga tggcactgaa tatgctacgc cgttggttaa
     1861 acggccagga cattgccagt gagcatggct ggattgaggt tgttgagtcc atgaccttat
     1921 ccgtctgatt ccgccactct gtgccggttt actgcaccgg cacattcccc ctccggcaaa
     1981 acttgtccaa cctgttgtac tcaataagaa aaaatccgtg cgctgattca caaatttgtg
     2041 cgtaatttct ccagtccgtt ggacgaggag cgggatttga tgcgcttcac aattttactc
     2101 cccctgagca acgagactga ttccagtatc taagactgga gtccagtatg ttggaatcaa
     2161 gcaaagttcc cgccctgaca cgggctattg atattctcaa tttgattgcc cgcattggcc
     2221 cctgtaatgc tgcgacgatc attgacacat tggggatccc taaaagcacg gcctatttgc
     2281 tgcttaatga actcaggcgt cagcgttttc ttagccttga tcaccaggaa aatttttgtt
     2341 tgtggaccaa gctggtggag ctttccggtc atgcgttaag caagatggat ctccgggaac
     2401 tggctcgacc gcgtttgacg caactgatgg ataccaccgg gctgttatgt cacctgggga
     2461 ttattgataa cggcagcgct tattacattt tgaaagtgga gtcatcagcc actatcagcg
     2521 tgcgttcaca tgaaggaaaa agcctttcgc tttatcgctc cggaataggt aaatgcctgc
     2581 ttgcctggca acctgcaact gtacagcaaa gtattatcga agggctggtc tgggagcagg
     2641 cgacacccac aacgattact catccacagc aacttcacga agaactggcg cgtattcgcc
     2701 gtcagggctg gagctatgac aacggcgaag attacgccga tgtccgttgt gtcgctgcgc
     2761 cggtgtttaa cgccaataac gagcttaccg ctgcgatctc ggtggtcggt acccgcttac
     2821 aaatcaatga agaataccgt gattatctgg cgggcaaggc cattgcctgc gccagggata
     2881 tttcacgtct gttgggatgg aaaagtccct ttgacttaca agcctcataa caatggagaa
     2941 catcatgacc ctaccaaaaa ttaaacaggt tcgcgcctgg tttactggcg gtgcgacagc
     3001 agaaaaaggc gctggcggcg gtgattatca cgaccagggg gcgaatcact ggatagacga
     3061 tcatattgcc accccgatga gtaaataccg cgattacgag cagttacgcc agtcatttgg
     3121 cattaacgtt cttggcacgt tggttgttga agtcgaagca gaaaacggcc agaccggatt
     3181 cgctgtttcg acagccggtg aaatgggctg ttttatcgtc gaaaaacacc ttaaccgttt
     3241 cattgagggt aaatgtgtca gtgatatcaa actgatccat gatcaaatgc tcaatgccac
     3301 cctgtattac tccggctctg gtggcctggt gatgaacacg atttcctgtg tcgatctggc
     3361 tctgtgggat ctgttcggca aagtggtcgg gcttccggtt tataaacttt taggcggcgc
     3421 tgttcgtgat gagattcagt tctacgccac aggtgcgcgt ccggatctgg caaaagagat
     3481 gggctttatc ggtggcaaaa tgccgacgca ctgggggcca catgatggcg atgcggggat
     3541 ccgcaaagat gccgctatgg tcgcggatat gcgtgaaaaa tgcggtgagg atttctggtt
     3601 aatgctcgac tgctggatga gtcaggacgt gaactatgcg accaaactgg cccacgcttg
     3661 cgcgccctat aacctgaaat ggagagtgtc tgccgccaca gcagtatgaa ggttatcgcg
     3721 aactgaaacg caacgcgcca gtcggaatga tggtcaccag cggtgagcac cacggcacac
     3781 tgcaatcttt tcgtacgctt tcagaaaccg gtatcgacat tatgcagccg gatgttggct
     3841 ggtgcggtgg cttaaccacg ctggtggaaa ttgccgcaat cgccaaatcc agggggcaac
     3901 tggtggtgcc gcacggttcg tctgtttact cccaccatgc ggtgatcacc ttcactaata
     3961 cgccattcag cgaattcctg atgactagtc cggattgttc aacgatgcgt ccgcagtttg
     4021 acccgattct gctcaatgag ccggttccgg tgaatggtcg tattcataaa tcagtgcttg
     4081 ataaacccgg ttttggcgtc gaactcaatc gtgactgcaa tttgaaacgc ccctacagcc
     4141 actaatcact ccgggcgttg ctgtgacaac ggtaacgccc acatattcta aggagctttt
     4201 tatgagcacc gctttgcttg acgccgtggt gaagaaaaac cgtgtgcgtt taattccgtt
     4261 tatgttggcg ctgtatgtgc tggcgtttct cgaccgttcg aatatcggtt ttgccaaaca
     4321 gacctaccag attgataccg ggttgagtaa tgaagcttat gcgctgggag caggcatttt
     4381 ctttgtggta tatgcgtttc tgggtgttcc ggcgaatctt ttgatgcgca aactgggggc
     4441 cagaacctgg attggtacga caacactgct gtggggattt ctttcggcag ccatggcatg
     4501 ggccgatact gaagcgaaat ttctgatagt tcgcactctg cttggtgctg cggaggccgg
     4561 atttttccct ggtatgattt atctcacctc gcaatggttt ccgcagcgta atcgcgccag
     4621 cattatgggg ctgttctata tgggcgcacc gctggcgtta acactgggat caccgctttc
     4681 cggcgcgctg ctggagatgc atggatttat ggggca
//
LOCUS       DAZKFZ010000277         4713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000277 DAZKFZ010000000
VERSION     DAZKFZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(253..1572)
                     /locus_tag="ACHOQW_004466"
     CDS             complement(253..1572)
                     /locus_tag="ACHOQW_004466"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP9575405.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1703..3238)
                     /gene="gadC"
                     /locus_tag="ACHOQW_004467"
     CDS             complement(1703..3238)
                     /gene="gadC"
                     /locus_tag="ACHOQW_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP9575406.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3394..>4713)
                     /locus_tag="ACHOQW_004468"
     CDS             complement(3394..>4713)
                     /locus_tag="ACHOQW_004468"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9575407.1"
                     /translation="ESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDEN
                     VHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEA
                     CMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQL
                     FMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAA
                     SGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNV
                     DYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLG
                     PYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1201 a         1178 c         1191 g         1143 t
ORIGIN      
        1 aggacagttt caatctacgc tactgttctt cagaagagta tagcccatcg taattatttt
       61 tcggtgactg cgaatatcgt atggtttttc atattcatac atttttatta ggggatttat
      121 ggctgtttaa ctaagcgtgg ttaattgcgt ggttaatttg actttagtaa gcatgattgc
      181 tggtgggata gttcaagagg gtaacaagcc agtgggtaaa gcaccggctt gttacaaagt
      241 aagaatggga gtttaactgc cccaacgact ttgcagatag ctgaccgctt gttgagtctg
      301 cggtttattc agatagtcct cacggaacaa gatggtgccg ctaatttctg gtaccgcatc
      361 gttaagatcg agctgctttt tcagctccgg tacgccgccg ttaatcatcc agtctggctc
      421 tatctttgaa ggttcaccca ctttatagaa ggcgataccg atatacaggc gggttctggt
      481 cggtttaacg acatccgccc accattttgc caacacgtca taacgcgcgg cactccgtga
      541 gaacggccag taaatttggg gagcaatgta atccagcaat ccttgttcca cccatcgacg
      601 agtgtcagcg taggattcgt cataggctgc cgcgcctcgg gtatcggaac cgagcggatc
      661 gtgtgatcgg ttacgccaca cgcctgccgg gctaacacca aattcgactc ccggcttaat
      721 gcttttaatg gtgtgcgata cctttgcaat taactgctga gtattgttgc gccgccagtc
      781 tgcttttgac gcaaatgcgc ctccgtattt acggtacgtt tcgttatcat ttagccgtga
      841 acccggtgac tcagtataga aatagtcgtc aaactgcacg ccatctaccg gatagcggga
      901 aaccacttct gcgactattg atgtgatcca gtcctgaacc tcagggatgc ccgggtcgag
      961 gacaaagcga tcgccagacg ttctgatcca gtcacggtgt tgcacataga cgctcgccgg
     1021 ttgttgagac agagtgctat tcagttccct gatagtaccg ggcttcgtat taaccgatac
     1081 gcgatagggg ttaaaccagg cgtgtacttt catcccacgc ttgtgggctt cgtcgagcat
     1141 aaattgcagc ggatcgtaac ccggattttc accaatctta ccggtcataa gatcggacca
     1201 cggcaaaatt ttcgatggcc acagggcggt accgtccggc ttgacctgga aaaagaccgt
     1261 gtttataccg agacgttgca aatgatccag tttgtcgatc atcgcctgtt gttgtacacg
     1321 ggcccggctg gtggggttac taatgttaac cgaggaaacc ggtggccagt cgaggcgaga
     1381 caccgtggcc agccagatgc cacgcatcgg ttgtgacgat tgttgcgtcg tggctggtgg
     1441 ctttgaaccc gctggtggtg tcaccatgga ctctggaggc gtgcttttac aactacacag
     1501 taaaagtgcc agtgcaacca gtatcgctgg tcttctaatc gttaatttct tgtttcggga
     1561 gcagatatcc atatgtgcta gtttccggta aacagatgtg cgctcattct cgtacttatc
     1621 ccggttaagt caatacgaca gcaagcacga aaaagggagc gatgaattat cgctccctta
     1681 tcttataacc attcagacat agttagtgtt tcttgtcatt catcacaata tagtgtggtg
     1741 aacgtgcacg cgggtgcagg aagaagtgac cttttggtgc gttctgactg ttgattggct
     1801 ccagagtgac gccggtattc gctttgcctt tacgatcatg aacagcatag agaataaagg
     1861 gcagggcaag taccaccagg aaactaacga ccagtaattc aacatacata tcggtagagt
     1921 caccctggat gttatccggc ggcaggaagg aaacaataaa cgccataatt gaagtcagca
     1981 gaccgacaat tgccacgacc agtttcaccc ctttaccacc agggatatta aatgtgcgtt
     2041 ttaagtcagg atgtttaaga accaacacaa tgtagccaat aaacagcatg aaataagcac
     2101 acagataaat caccaccgtc agcgccagtg cgatcaggaa ggacatgttg ttaccgccac
     2161 cggtattggt gaggatgatc aacgcgatag aagtaatcac cagctgcgaa atgaccagcg
     2221 ttaccggtac gccatttttg ttcattttag cgaatgccgc tggcagcagg tttttctgcg
     2281 ctgtgacata catcccgcga gaaggaccaa caatccagga ggcgatttcc gccagaacac
     2341 ccagcaacag cagtgcggag atcacgcgaa ccgtccactc aatttccggt gccacatggg
     2401 acatcagaac ggtaaaggtt tgcattaccc ctgcggagag gttgatttca ttacccggaa
     2461 tgaccatcgc aatagacaaa ccgccaacag agcttaagca gattgccgcc accatcagca
     2521 gtaacatagc cagtggatag tcgcgccctg ggttactcat ttcattgacg tgggttgcgg
     2581 aagcttctac gcccatataa ctcaaaatga aggcaacaaa tacaaccagg gtgcccactt
     2641 tagagaagtc agggaagaag gtcttcgaat ccatttcgat agcaacgggg gcaccggagt
     2701 gcagataaat agccgctaat gcaatcaaaa taaatgcagg taacaggata ccggcgaaga
     2761 agccaacttt agcaattcgc gccgtgtatt tcgtgccacc aaactgcgtt aatgccagcg
     2821 cccaaagaat gatgagtgct gcaatagttt tggtaatggg gtcttcattc agcgctggcc
     2881 atttcaggat gtaggagagt gcccctaaca cgaaatagag catcggaata aaaccaatgg
     2941 cgatttgcag atagccaaat gagatcgctg caaatcccca tctcggcccc agagtatttg
     3001 atacccaggc gaagacacca ccttcttccc agccgtcgac ggtggccatt tccgcagcac
     3061 aaagtcccac gggaataaac cataaaatcc cgcctaatag caggaagaag actaatgaaa
     3121 agcccgatgt tgcgaaggta gggtattcat aaacagccat taccatcgat gccgttatgg
     3181 caaaaaagcc gagtaatgtg agctgcttag ctttacctgt ctgtactaat gtagccatat
     3241 tatcccccta aaacggtatt cctgtcggaa ccgcacctgt tttgttctga agcgtattca
     3301 gaacaatatt ttccgttgct aatgccagtg aacagacttt ggaaattgtc ccgaaacggg
     3361 ttcgtttcgg gacaccgtta ccgttaaacg ttatcaggta tgtttaaagc tgttctgttg
     3421 ggcaataccc tgcagtttcg ggtgatcact gagatatttc agggaggctt tgtagtcttc
     3481 cagcaacagt tcagcaaagt ccatttcgaa gccgcgacga cacataatgc gcatcaccac
     3541 gatgtcggtg gcttcaccgc cgagagtgaa ggccggaacc tgccagccgc gcagacgcag
     3601 acgttcagag aggtcataca gggtgtatcc cggatcttca ccatctttca gtttgaagca
     3661 aaccgccgga atgccttcgt ccgggcgacc ggtacagatg aactcatacg gccccagttt
     3721 ggcgatttca tccgccagat aagcggcaac ctggtaagag gcgttctgta ctttggtata
     3781 gccttcacga ccgaggcgca ggaattcata gtactgtgca attacctgac ccgccgggcg
     3841 ggagaagttg atggcgaaag taccaatctg accaccgagg tagtcaacgt tgaacaccag
     3901 ttcctgcggc agcgcttctt catcacgcca gataacccag ccgcagccca gcggagccag
     3961 accgaatttg tggcctgaag cactgatcga tttcacacgc ggcaggcgga agtcccagac
     4021 gatatccggg gcgacgaacg gtgccaggaa gccaccactg gcggcgtcga tgtgcatgtc
     4081 gatgtcgata ccggtgtcgg cctggaattt atccagcgca tcgtgcagcg gttgcgggaa
     4141 ctcatagtta ccggtgtagg tcacgccgaa agtcggcacc acgccgatgg tattttcgtc
     4201 gcaggcttca atcatgcgtt tcggatccat aaacaactga ccggggcgca tagggatctc
     4261 acgcagctcc acatcccagt agcgggcgaa tttatgccag cagatttgca ccgggccgca
     4321 caccaggttt ggtttatccg ttggtttgcc tgcagcttcc atacgcttgc gccaacgcca
     4381 tttcatcgcc atcccgccga gcatacaggc ctcggaagaa ccaatggtgt tggtgccaac
     4441 ggcctgacca tttttcggcg caggcgcatg ccacagatcg gcaaccatat ttacgcaacg
     4501 caggtcgatg gctgcagatt gcggatattc ttctttgtcg atccagtttt tgttaatgga
     4561 taaatccatc aacttgtgga cgttttcgtc gtcccaggtc tggcagaaag tggccaggtt
     4621 ctgacgagcg ttgccatcaa gatataattc gtcattgata atctgaaatg cgacatcgtc
     4681 gcgcatttcg tgcagcggaa aacgttttga ttc
//
LOCUS       DAZKFZ010000278         4689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000278 DAZKFZ010000000
VERSION     DAZKFZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1135
                     /gene="proV"
                     /locus_tag="ACHOQW_004469"
     CDS             <1..1135
                     /gene="proV"
                     /locus_tag="ACHOQW_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP9575408.1"
                     /translation="YIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGK
                     STMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLD
                     NTAFGMELAGINAEERREKALDALRQVGLENYAHSYPNELSGGMRQRVGLARALAINP
                     DILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNG
                     EVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSA
                     LKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLS
                     ELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1128..2192
                     /gene="proW"
                     /locus_tag="ACHOQW_004470"
     CDS             1128..2192
                     /gene="proW"
                     /locus_tag="ACHOQW_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP9575409.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2249..3241
                     /gene="proX"
                     /locus_tag="ACHOQW_004471"
     CDS             2249..3241
                     /gene="proX"
                     /locus_tag="ACHOQW_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP9575410.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3433..4617
                     /locus_tag="ACHOQW_004472"
     CDS             3433..4617
                     /locus_tag="ACHOQW_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9575411.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
BASE COUNT         1033 a         1240 c         1261 g         1155 t
ORIGIN      
        1 atatatcgaa caaggacttt caaaagaaca aattctggaa aaaactgggc tatcgcttgg
       61 cgtaaaagac gccagtctgg ccattgaaga aggcgagata tttgtcatca tgggattatc
      121 cggctcgggt aaatccacaa tggtacgcct tctcaatcgc ctgattgaac ccacccgcgg
      181 gcaagtactg attgatggtg tggatattgc caaaatatcc gacgccgaac tccgtgaggt
      241 gcgcagaaaa aagattgcga tggtcttcca gtcctttgcc ttaatgccgc atatgaccgt
      301 gctggacaat actgcgtttg gtatggaatt ggccggaatt aatgccgaag aacgccggga
      361 aaaagccctt gatgcactgc gtcaggtcgg gctggaaaat tatgcccaca gctacccgaa
      421 tgaactctct ggcgggatgc gtcaacgtgt gggattagcc cgcgcgttag cgattaatcc
      481 ggatatttta ttaatggatg aagccttctc ggcgctcgat ccattaattc gcaccgagat
      541 gcaggatgag ctggtaaaat tacaggcgaa acatcagcgc accattgtct ttatttccca
      601 cgatctcgat gaagccatgc gtattggcga ccgaattgcc attatgcaaa atggtgaagt
      661 ggtacaggtc ggcacaccgg atgaaattat caataatccg gcgaatgatt atgtccgtac
      721 cttcttccgt ggcgttgata ttagtcaggt attcagtgcg aaagatattg cccgccggac
      781 accgaatggc ttaattcgta aaacccctgg cttcggccca cgttcggcac tgaaattatt
      841 gcaggatgaa gatcgtgaat atggctacgt tatcgaacgc ggtaataagt ttgtcggcgc
      901 agtctccatc gattcgctta aaaccgcgtt aacgcagcag caaggtcttg atgcggcgct
      961 gattgatgcg ccgttagcag tcgatgcaca aacgcctctt agcgagttgc tctctcatgt
     1021 cggacaggct ccctgtgcgg tgcccgtggt cgacgaggac caacagtatg tcggcatcat
     1081 ttcgaaagga atgctgctgc gcgctttaga tcgtgagggg gtaaataatg gctgatcaaa
     1141 ataatccgtg ggataccacg ccagcggcgg acagtgccgc gcaatccgca gacgcctggg
     1201 gtacaccggc gactgcaccg actgacggcg gtggtgctga ctggctgacc agtacgcctg
     1261 cgccaaacgt cgagcatttt aatattctcg atccgttcca taaaacgctg atcccgctcg
     1321 acagttgggt cactgaaggg atcgactggg tcgttaccca tttccgtccc gtcttccagg
     1381 gcgtgcgcgt tccggttgat tatatcctca acggtttcca gcaattgctg ctgggtatgc
     1441 ccgcaccggt ggcgattatc gttttcgctc tcatcgcctg gcagatttcc ggggtcggaa
     1501 tgggcgtggc gacgctggtt tcgctgattg ccatcggcgc aatcggtgcc tggtcgcagg
     1561 caatggttac tctggcgctg gtgttaaccg ccctgctgtt ctgtatcgtc atcggtttgc
     1621 cgttggggat ctggctggcg agaagtccgc gagcggcgaa aattattcgt ccactgcttg
     1681 atgccatgca gaccacgcca gcgtttgttt atctggtgcc aatcgtcatg ctatttggta
     1741 tcggtaacgt gccgggcgtg gtggtgacga tcatctttgc tctgccgccg attatccgtc
     1801 tgaccattct ggggattaac caggttccgg cggatctgat tgaagcctcg cgctcattcg
     1861 gtgccagccc gcgccagatg ctgttcaaag ttcagttacc gctggcgatg ccgaccatta
     1921 tggcgggcgt taaccagacg ctgatgctgg ccctttctat ggtggtcatc gcctcgatga
     1981 ttgccgtcgg cgggttgggt cagatggtac ttcgtggtat cggtcgtctg gatatggggc
     2041 ttgccaccgt tggcggcgtc gggattgtga tcctcgccat tatcctcgat cgtctgacgc
     2101 aggccgttgg gcgcgactca cgcagtcgcg gcaaccgtcg ctggtacacc actggccctg
     2161 tcggtctgct gacccgccca ttcattaagt aactctgcac ttgcccggtg acgccgggca
     2221 tatcaccctg ccaaaaaaag gaataacaat gcgacatagc gtactttttg cgacagcgtt
     2281 tgccacgctt atctctacac aaacttttgc tgccgacctg ccgggcaaag gcattactgt
     2341 taatccggtt cagagcacca tcaccgaaga aactttccag acgctgctgg tcagccgcgc
     2401 gctggagaaa ttaggttata ccgtcaataa acccagcgaa gtggattaca acgttggcta
     2461 cacctcgctt gcttccggcg atgcaacctt caccgccgtg aactggacgc cactgcatga
     2521 caacatgtac gaagctgccg gtggcgataa gaaattttat cgtgaagggg tatttgttaa
     2581 cggcgcggca cagggttacc tgatcgataa gaaaaccgcc gaccagtaca aaatcaccaa
     2641 catcgcacaa ctgaaagatc cgaagatcgc caaactgttc gataccaacg gcgacggaaa
     2701 agcggattta accggttgta accctggctg gggctgcgaa ggtgcgatca accaccagct
     2761 tgccgcgtat gaactgacca ataccgtgac gcataatcag gggaactacg cggcgatgat
     2821 ggccgacacc atcagtcgtt acaaagaggg caaaccggtg ttttactaca cctggacgcc
     2881 gtactgggtg agtaatgagc tgaagccagg caaagatgtg gtctggttgc aggtgccgtt
     2941 ctctgcactg ccgggcgata aaaatgccga taccaaactg ccgaatggcg cgaattacgg
     3001 cttcccggtc agcaccatgc atatcgttgc caataaagcc tgggccgaga aaaacccggc
     3061 agcagcgaaa ctgtttgcca ttatgcagtt gccagtggca gatattaacg cccagaacgc
     3121 cattatgcat gacggcaaag cctcagaagg cgatattcag ggccacgttg atggctggat
     3181 caaagcccac cagcaacagt tcgatggctg ggtgaatgag gcgttggcag cgcagaagta
     3241 atttttattc gggcggataa ggcgtttacg ccgcatccgc cgttctgtac acaatgcctg
     3301 atgcgacgct ggcgcgtctt atcatgccta caaaccacac cgcacatttc aacaatctat
     3361 ctttcatccc atattcatca acatccgcta ttattgattt ccagcttaat catcacctga
     3421 tgaacaaaaa taatgaccaa acctaatcat gagcttagcc cggcgctaat cgtgctgatg
     3481 tctatcgcca ccggtctggc ggtcgccagc aactattacg cccagccatt gctcgacacc
     3541 atcgcgcgta acttttccct ttccgccagt tcggcaggct ttattgttac cgccgcgcag
     3601 ttgggctatg ccgccggtct gctgtttctt gttcccctcg gtgatatgtt tgaacgccgc
     3661 cgcctgattg tctcgatgac cttactggca gcgggcggta tgttaatcac tgccagcagt
     3721 cagtcactgg cgatgatgat cctcggtacg gcattaaccg gtttattctc agtcgtggca
     3781 caaattctgg ttccgctggc agcgacgctg gcttcaccgg ataaacgcgg caaagtggtt
     3841 ggcaccatta tgagcggtct gctgttgggg atattgctgg cacgaaccgt tgccgggtta
     3901 ctggcaaacc tcggcggctg gcgcaccgtc ttttgggttg cctcggtgtt aatggctctg
     3961 atggcgctgg cattatggcg tggtctgcca caaatgaaat cagaaaccca cctcaactac
     4021 ccacagttgt tgggttccgt tttcagcatg tttatcagca ataagatcct gcgcacccgc
     4081 gcgttgctgg gctgcctgac ctttgccaac ttcagcattc tctggacctc aatggccttt
     4141 ttgcttgccg ctccaccttt taactacagc gatggtgtaa ttggtctgtt tgggcttgcg
     4201 ggagctgccg gagcgttggg cgctcgtccg gcgggcggtt ttgccgacaa gggcaaatcg
     4261 caccacacca caactttcgg tctgcttctg ctgttacttt catggctggc aatctggttt
     4321 ggtcacactt ccgtactggc gttgattatc ggcattctgg tgctggacct caccgtacag
     4381 ggcgtgcata tcactaacca gacggtaatt tatcgaatac atcctgatgc gcgtaatcgc
     4441 ctgaccgcag gttacatgac cagctacttt attggcggtg ccgccggttc gctaatttca
     4501 gcctcagcct ggcaacatgg cggttgggct ggcgtttgtc tggctggcgc gacgattgcc
     4561 ctggttaact tactggtctg gtggcgaggt tttcatcgtc aggaagccgc aaattaagca
     4621 atcagcaacc gttttcgtgg ctttacactt ataagggtgt taagaagccc atcagtctga
     4681 taaggttaa
//
LOCUS       DAZKFZ010000279         4675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000279 DAZKFZ010000000
VERSION     DAZKFZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(141..356)
                     /locus_tag="ACHOQW_004473"
                     /pseudo
     CDS             complement(141..356)
                     /locus_tag="ACHOQW_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(400..966)
                     /locus_tag="ACHOQW_004474"
                     /pseudo
     CDS             complement(400..966)
                     /locus_tag="ACHOQW_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            complement(978..1382)
                     /locus_tag="ACHOQW_004475"
                     /pseudo
     CDS             complement(978..1382)
                     /locus_tag="ACHOQW_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1382..1567)
                     /locus_tag="ACHOQW_004476"
     CDS             complement(1382..1567)
                     /locus_tag="ACHOQW_004476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575412.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            1744..3954
                     /gene="katP"
                     /locus_tag="ACHOQW_004477"
     CDS             1744..3954
                     /gene="katP"
                     /locus_tag="ACHOQW_004477"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFP9575413.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3998..4387
                     /locus_tag="ACHOQW_004478"
     CDS             3998..4387
                     /locus_tag="ACHOQW_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9575414.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            4444..4675
                     /locus_tag="ACHOQW_004479"
                     /pseudo
     CDS             4444..4675
                     /locus_tag="ACHOQW_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    4500..4616
                     /locus_tag="ACHOQW_004479"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1283 a         1043 c         1175 g         1174 t
ORIGIN      
        1 ataaaaagaa cagatatgcg agttatgatc ttgatcgata ggaatttaaa tcccaaaaag
       61 attaaaaaaa cacccaaaaa cggatgttta ttcaacactg cctgcgtgcc atgagtgcac
      121 cgccgtcaat taaatggatc tatttcatcc tgaccagcat agtgctccag acggctcatc
      181 ggcaccggcg gttttttcag gtaagagccg aagtacaccg ccacatgtgt ggcgttatcc
      241 atcacccggg atacgttgac attccagccc cgccggtaat gactgtccag gaagcgattc
      301 cagtcccgtt tactgcttcc ttctgctgcc agcgcatccg gtatcaccag gtccggtgag
      361 gtgtactggc aatagcggac actaccattt gttctttttt taagcagcca tctgatgata
      421 tttttccctg aaggctgccg gggagatatt ccccagacga gagtgacgac gctgacgatt
      481 gtagaaaatc tcaatgtatt cccgtattac tgagatggct tcatcccggt tattaaaacg
      541 atagtggctc aggctctcat ttttcagcgt tccccagaag ctttccatcg gagcgttgtc
      601 gtaacagtta cctttacgcg acattgatgt tttcagacca aactgctcct gtatgacccg
      661 gtaatcgtat gcgcagtact gtgaacctcg atcagagtgg tggattagcc cggcaggtgg
      721 gcgctggctc ctgagcgcca taaacagggc tttacctgtc agctcttttg tcatgcgctc
      781 tcccatggcg tagccgacaa tttcgcacgt ataaacatct ttgatgccag cgaggtacaa
      841 ccatccctcc tgtgtggcaa catacgtcag gtccgccacc cagacctgat ttggtgctgc
      901 aggagcgaac gtctggttca gcagatttgg cgcaactggc agattgtggt tcgagttcgt
      961 agtcgcacat ctgcctagcg gcagatttcc tgtatcacat ccgcagcaat gcggctcatc
     1021 tctgccagta accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac
     1081 acaatatgct gccacggaca gtcgggaacc agactcagca gatactgtat ccactgtgcg
     1141 ccagccttca ctccgcagtg cgggcaggag cgacttttac accggaagca gacctttttt
     1201 atgtggctgc agtccggaga tgaacagcac cactgtgtgt accccatcag cgtggtcccg
     1261 caggccatga tttttgtcac agactcaatg actaccggac gcactgaacc ttccggttgt
     1321 ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tgtcggcaaa acggggaagc
     1381 atcagaaggg cgggggaact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca
     1441 tgccgccggc gctgataccg gcaagaatgg tcgcaaactc ccgctccgtg cagcgggcta
     1501 tttcaggata cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttctga
     1561 catccacaaa gaagggaata ttcaggtctg cgcagcactc aacggcatcg tcagttgcgg
     1621 cttggaaccc cttagtattt tttgtctgta gtatctatcc cagcaatagg tatatcctgt
     1681 tgcatcaata aagttgactt ttgtatacaa catgcgaatt tcccttaatc cggagctatt
     1741 cgtatgataa aaaaaactct tcctgttctg attcttctgg cgctatcggg gagcttttct
     1801 accgctgtag ccgctgataa aaaagagact caaaatttct actatccaga aacactggat
     1861 ttaactcctc tgagattaca cagccctgaa tcaaatccct ggggggctga ttttgattat
     1921 gccaccagat ttcaacagct ggatatggag gctctgaaaa aagatatcaa agatttgctg
     1981 acaacttccc aggattggtg gcctgcggat tatggtcatt atggtccttt ctttattcgt
     2041 atggcttggc acggtgccgg aacatacagg acatatgatg gccggggagg cgccagtggt
     2101 ggtcagcaac gttttgaacc gctgaacagc tggccggata acgttaatct ggataaagcc
     2161 cgtcgattgc tgtggccagt caagaaaaaa tacggctcca gtatttcctg gggagacctg
     2221 atggtcctga ctggtaatgt tgcccttgaa tccatgggat ttaaaacgct gggatttgct
     2281 ggcggaagag aagatgactg ggagtcggac ctggtatact gggggcctga caacaagcct
     2341 cttgcagata accgggataa aaacgggaaa cttcagaaac ctcttgccgc cacgcagatg
     2401 ggacttattt atgtcaatcc tgaaggcccc ggtggaaaac cagatcctct ggcttccgcg
     2461 aaagatatca gggaagcttt ttcacgtatg gccatggatg atgaggagac tgtggccctg
     2521 atcgcgggag ggcatacatt tggtaaagca catggtgcag cgtctcctga aaaatgtatt
     2581 ggcgcagggc ctgatggtgc acctgtggag gagcagggac tgggatggaa aaataaatgt
     2641 ggtacaggaa acggcaaata taccatcacc agtggcctgg aaggagcctg gtcgacatcg
     2701 ccaacccagt tcacaatgca gtatctgaag aatttatata aatatgaatg ggagctgcac
     2761 aagagtcctg ccggtgctta tcagtggaag cctaaaaaag cggcaaatat agttcaggac
     2821 gcgcatgatc cgtctgtcct gcatccgttg atgatgttta cgacggatat tgctcttaaa
     2881 gttgatcctg aatataagaa aataaccacc cgtttcctga atgatccaaa agcttttgag
     2941 caggcattcg caagagcatg gtttaaactg acccaccggg atatgggacc ggcagcccga
     3001 tatcttggta atgaagttcc tgcagaatca tttatctggc aggatcctct tcctgcggcg
     3061 gattatacaa tgattgatgg taaagacatt aagtcgctga aagagcaggt tatggatttg
     3121 ggtatccctg catctgagct gataaagaca gcctgggctt cagcttccac atttcgtgtg
     3181 actgattatc gtgggggaaa taatggtgcc cgcatcaggt tacagcccga aattaactgg
     3241 gaagttaatg agcctgaaaa actgaagaaa gtactggcat ccctgacctc attacagcgt
     3301 gaatttaaca aaaaacagtc tgacggaaag aaagtgtcgt tggctgattt aattgttctt
     3361 tcgggtaatg ctgcaatcga agatgcggcc agaaaagccg gggtggaact tgagattccc
     3421 tttactccgg gaagaactga cgcctctcag gagcagacgg atgttgcctc attcagtgta
     3481 ctggagccga cagcagatgg attcagaaat tattactcaa aaagcagaag tcatatatcg
     3541 ccggttgaaa gcctcattga taaagccagt cagctggatc tcaccgttcc tgaaatgacg
     3601 gcattactgg gtggtctgcg ggtaatggat attaatacaa ataattcttc gttgggagtg
     3661 tttaccgata cccctggtgt tctggataac aagttttttg ttaatctgct ggatatgtca
     3721 acacgatgga gtaaagcaga taaagaagat acatacaatg gattcgatcg taaaacggga
     3781 gcattaaaat ggaaagcatc ctctgttgat ttaatcttca gttcaaatcc tgaattacgt
     3841 gcggtggcag aagtatatgc ctcggatgat gcgagaaata agtttattca tgattttgtt
     3901 aaatcgtgga ataaagttat gaatagcgat cggtttgatt taaacaataa atgataaaac
     3961 tgatgaggga gggcacctcc ctcatgaagg agaaattatg cagaaaagca ataaagttat
     4021 aatcagctgt ggtatggcat tatatttttt gtgtgggggg attacagtgg cagaagaact
     4081 tgatgaaagc atgcaagttc ttgcaaggaa tatgagtgta cttcagtcat caatggataa
     4141 aaaggagctt cttgaagctc ttgacagaat ggatgatgct gtagatgagt caatgaagcg
     4201 tcttcctgag agcatttcac cggctgatga agaagggaag aatgactata taaaagaact
     4261 tcaaaagctt gaaaaagaga ttatgctggc gaaacaaaaa gtcttatcgg gacagttatc
     4321 agatataccc gattcggtaa acaagatgaa taaaatcaga gttgaggggc atgataaatt
     4381 cagataaatc agttggttga ttcctgcggg acgaatacat acgtcaggaa cataacttta
     4441 tggctggaac gtgaaaatcg tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc
     4501 gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aataatgcca ctgctggata
     4561 agctgcgtga gcagtacggg gtcggaccgg tatgcagtga gctgcatatt gccccgtcaa
     4621 cgtattacca ctgtcagcaa cagcgacatc atcctgataa acgcagtgcc cgtgc
//
LOCUS       DAZKFZ010000280         4623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000280 DAZKFZ010000000
VERSION     DAZKFZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..141)
                     /locus_tag="ACHOQW_004480"
     CDS             complement(<1..141)
                     /locus_tag="ACHOQW_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575415.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQR"
     gene            complement(125..355)
                     /gene="dicC"
                     /locus_tag="ACHOQW_004481"
     CDS             complement(125..355)
                     /gene="dicC"
                     /locus_tag="ACHOQW_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HFP9575416.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGELVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            439..846
                     /gene="dicA"
                     /locus_tag="ACHOQW_004482"
     CDS             439..846
                     /gene="dicA"
                     /locus_tag="ACHOQW_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575417.1"
                     /translation="MDTKNLTIGERIRYRRKNLKYTQRSLAKVLKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPSPPIEEPVALSPKEHELLELFNALPES
                     EQDAQLTEMRARVKNFNKLFEELLKARQRTNKR"
     gene            1013..1168
                     /locus_tag="ACHOQW_004483"
     CDS             1013..1168
                     /locus_tag="ACHOQW_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9575418.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            1170..1298
                     /gene="ydfB"
                     /locus_tag="ACHOQW_004484"
     CDS             1170..1298
                     /gene="ydfB"
                     /locus_tag="ACHOQW_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFP9575419.1"
                     /translation="MDFDTIMEKAYEEYFEGLADGEEALSFNEFKQALSSSAKSNG"
     gene            1328..1546
                     /gene="ydfC"
                     /locus_tag="ACHOQW_004485"
     CDS             1328..1546
                     /gene="ydfC"
                     /locus_tag="ACHOQW_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9575420.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     KLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(1550..1714)
                     /locus_tag="ACHOQW_004486"
     CDS             complement(1550..1714)
                     /locus_tag="ACHOQW_004486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575421.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRTHSTSPVEN
                     YLITNLSFVG"
     gene            2112..2300
                     /gene="dicB"
                     /locus_tag="ACHOQW_004487"
     CDS             2112..2300
                     /gene="dicB"
                     /locus_tag="ACHOQW_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9575422.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            2297..2485
                     /locus_tag="ACHOQW_004488"
     CDS             2297..2485
                     /locus_tag="ACHOQW_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9575423.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNEIPAG"
     gene            2578..>4623
                     /locus_tag="ACHOQW_004489"
     CDS             2578..>4623
                     /locus_tag="ACHOQW_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFP9575424.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASSDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LHEARKNIPPQNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQNQPETEQ
                     NDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVMGDATYQ
                     ETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHRII
                     TSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVID
                     "
BASE COUNT         1354 a         1100 c         1106 g         1063 t
ORIGIN      
        1 gcgttgaaca tcgatgttcg ggcgttcttt cagagcccag aatgcttccg tgattaatat
       61 cgtcgcctgt tcctgtgtca ttcctggtcg gcacacccag gcatccagag cctcacaaac
      121 ctgttcaggg gtgattttca ttgttcaacc gccccgcccg ctttgcctta cgatattcgt
      181 cataaacttt ggggtcgtac tgaagttccc cgccggatgc ctcttgcagg cgcatcgcgc
      241 gaccttcagg tactagctcc cctttccagc tataaagcga agccaaacga ataccagcag
      301 cttgtgcaag ttttgttttt gaaccgaaat acaaaagtgc gtcagtttta agcatttaaa
      361 gcaccttaat tgttagccat gactaacaaa ataaatgtta acaaaaacat agtcaataag
      421 atttagcatt agctaactat ggatacaaaa aatttaacca ttggcgaacg cattagatat
      481 cgccgaaaaa acctcaaata cacccaaagg tctcttgcta aagtcctgaa aatttctcac
      541 gtatcggttt cacaatggga gcgggatgat agcgaaccta caggaaagaa tctttttgcc
      601 ctcagcaaag tactgcagtg ctcgccaaca tggattctat ttggcgatga agacaagcag
      661 ccatcaccac ctattgagga gccagttgct ctatccccca aagaacatga gctccttgag
      721 ctttttaatg cattgcctga atcagaacag gatgctcagc tcaccgagat gcgcgcccga
      781 gtaaaaaatt tcaacaaact ctttgaagag ttattaaaag cccgtcaacg aacaaacaaa
      841 agataacgca atcaatgcgt tatctttttg gttgcccaaa atgttagcca tgacaaacaa
      901 aaatacttga ccaaactgtt agtcatggct aatcttgctt gcatcaagac accgcacggt
      961 gttctcagca aacagttccg ctaccccggc gttaagggga aatgaggtca gcatggatac
     1021 tatcgatctt ggcaacagcg aatctctggt atgtggcgtg ttccccaacc aggacggcac
     1081 gttcaccgcg atgacgtata ccaaaagcaa aacgtttaaa accgaaaatg gtgcccgtcg
     1141 ctggctggaa agaaactcag gtgagtgata tggatttcga cacaatcatg gaaaaggctt
     1201 atgaagaata cttcgaaggt cttgccgacg gcgaagaagc tctcagcttc aacgaattta
     1261 aacaggcgct ttccagctcg gcaaaatcta acggctgata agcgaaacag caccgcgagg
     1321 aatcagtatg cagaaacgag aacccgtcat catcgcacca gactataccg atgatgaact
     1381 ttatgagtgg atgcgccaga aaattaatgc agcgcaggat ctgaaatggg ccaatgaagc
     1441 cagggctaag caggctgaaa aactgtccgc tctggagcag gatatcacca atctggaaaa
     1501 agcagcggca ttaagcattg ccagaatgat tacatacccg cgttaatagc taaccaacga
     1561 agctaaggtt ggtaattaag tagttctcca cgggtgaggt ggagtgcgtg cgccggacgc
     1621 gggtgagcat ccggcactga cagtttactg aaaggatatt tccctgaaaa gtcagaccat
     1681 aacgcgaaag cgcacggcga ggtagctggt tcatagatag cctgtcgtta aattttcgtc
     1741 gaccgtgcgc ttccggttgt ggcaacccgc gaaatggtgc ggcggtaagt atggcggggt
     1801 tatttcttcc ccgttgagga caccgggttg tcaggttgac catacgctta agtgacaacc
     1861 ccgctgcaac gccttctgtt atcaattttc tggtgacgtt tggcggtatc agttttactc
     1921 cgtgactgct ctgccgccct ttttaaagtg aattttgtga tgtggtgaat gcggctgagc
     1981 gcacgcggaa cagttaaagc taaaaacagc gttatgggtg attctgtatt ccggcgttaa
     2041 ttgttaactg gttaacgtca cctggaggca ccaggcacca catcacaaaa ttcattgttg
     2101 aggacgcgat aatggaaacg tcactaccaa acgttaatac gtctgaaggg tgttttaata
     2161 ttggtattct gctcagtaac cgggagttta ctgaagacgc catcaggatg agaaaatatg
     2221 agccttatct tctcaatgat aattccatac tctccagaat tgcccttctt gaacttggca
     2281 ttttcggagg gcagcagtga gttcagcgtt tgcactgatg atgacggttt ttcttataac
     2341 aggtgagcca cagaatgtga ttaccggaat ttatgccagt aaagaatcct gccatcaggc
     2401 aagagacgag caaaaaattt ccggtgaatg tctcccgtta aacaaagtat cgctgtacct
     2461 gaataacgaa ataccggctg gataacccgc cagccgtatt aacgccatac ccgttgatta
     2521 agcatgccag caatggcagg gattcgtaca accttaaaat agttatgagg tttatcaatg
     2581 agcactgata aagaagaaat tgcactgtat tacgaagcca aaaatgacaa agtcagaaaa
     2641 cgccttggga ttaaaggcgg tttttactgg cgcacagcaa aaaaattatc ggttgcaata
     2701 tcacggggtg ttgtcgcaat ggacgatgct ggatttgacg aagaggattt caaaaaacct
     2761 gttcgcgtga atttgcccat tgttaatgac ctgccgcctg aaggtgtgtt cgatactgaa
     2821 ttctgcaacc gctatgaaaa aggcggggaa gatggcatca caatgatatt tatagcgcct
     2881 tccccctcag ttcaggacaa accagccagc tctgacaata ccaacgtcaa tggcgaagac
     2941 atggctgaga ttgaggataa tatgctcctg ccgatttccg gtcaggaact gcccattcgc
     3001 tggcttgcgc aacatggcag cgaaaaaccg gtaacgcacg tttcacggga agaacttcag
     3061 gcattacata tcgcacgagc tgaagaactg cctgctgtta ctgccctggc tatttcccac
     3121 aacacaaagc tgctcgaccc gctggagatt cgcgaccttc acaaactggt acgcgacaca
     3181 gacaaagttt tccctaatcc cgttaattcc agtctggggt taatgactgc ttttttcgaa
     3241 gcatacctgg acgctgacta taccgatcga ggtctgctga caaaagagtg gatgaaagga
     3301 aatcgtgttt tacgcatcag ccgcacgcca tccggcgcta atgctggcgg aggaattctt
     3361 accgatcgcg gtgaaggttt tgtccacgat gatgcgtcag tagaacgtga cgttgccgct
     3421 ggcgttctgg cccgttcaat ggacatcgat atttacaatc cacatccggc acacgccaaa
     3481 cgcattgaag aaatcgtttc agagaataag ccgccctttt ctgtttttcg tgacaaattc
     3541 atcgccatgc ctggtcacct ggattattcc cgcgcgatag tggttgcgtc cgtgaaagaa
     3601 gcaccaattg gtatcgaggc tactccccac cgtgttaccg aatatctgaa caaagtactg
     3661 accgaaaccg accatgccaa ccctgatcca gaaatcgtgg atattgcctg cggtcgctcc
     3721 tctgctccaa tgccgcagcg tgtaacaaaa gaaggaaaac aggatgatga agaaaaaccg
     3781 cagccatctg gcgcaatggc agatgaacag gcaacgactg aagcagtgga accggataca
     3841 actgaacata atcaggacac gcagtcgatg gatgctcagc cacagataaa ttctgttgat
     3901 gcgaaatatc agaaactgcg ggcagaactc catgaagccc ggaaaaacat tccgccccaa
     3961 aatcctgtcg atgcagacaa attactggct gcctctcgcg gagaatttgt tgaagggatt
     4021 agcgacccga atgatccgaa atggattaag gggatccaga cccgcgattc tgtgtaccag
     4081 aatcagccag aaacggaaca gaacgaccag aaagcggaac agaacagccc aaatacgcaa
     4141 caaaacgagc cagaaacgaa acaacctgaa ccagtagtgc aacaggaacc ggaaaagatc
     4201 tgcaccgcct gcggtcagag gagtggcggc aactgccctg attgtggcgc ggtgatgggc
     4261 gacgcaacat accaggaaac attcgatgac aagaacctgg ttgaagttca ggaagacgat
     4321 tcggagaaaa tggaaggcgc tgaacatcca cacaaggaga atgctggcag cgctcaggac
     4381 cacgccagcg atagtgaaac tggcgagacg gcagatccct taattacggt gaacggtcat
     4441 cgcattatca catccaccag caggacgtgt gaccatctaa tgatcgacct tgaaaccatg
     4501 ggaaaaaatc ctgatgcccc gattatctca ataggtgcaa tatttttcga tccgcaaacc
     4561 ggagatatgg gaccggaatt tagtaagact atcgatctgg aaactgctgg cggagtcatt
     4621 gat
//
LOCUS       DAZKFZ010000281         4529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000281 DAZKFZ010000000
VERSION     DAZKFZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..446)
                     /locus_tag="ACHOQW_004490"
     CDS             complement(<1..446)
                     /locus_tag="ACHOQW_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9575425.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVA"
     gene            complement(392..613)
                     /locus_tag="ACHOQW_004491"
     CDS             complement(392..613)
                     /locus_tag="ACHOQW_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575426.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(658..2595)
                     /locus_tag="ACHOQW_004492"
     CDS             complement(658..2595)
                     /locus_tag="ACHOQW_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9575427.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(2659..4320)
                     /locus_tag="ACHOQW_004493"
     CDS             complement(2659..4320)
                     /locus_tag="ACHOQW_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9575428.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(4317..>4529)
                     /locus_tag="ACHOQW_004494"
     CDS             complement(4317..>4529)
                     /locus_tag="ACHOQW_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575429.1"
                     /translation="AMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDA
                     AKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT          926 a         1486 c         1167 g          950 t
ORIGIN      
        1 tccgccacca gaggttcaac ccggctccag tccagaatac gcagttcttt gatctgcccc
       61 cgggcattac ggattttcag caccaccgta ttgccatgac gcagtttggc gttcagccac
      121 agttcaaaaa actggatgcg gttctgctgg gcgttgggac gacgacagag gcgggcaata
      181 tccccccggc gcgtttccct gcgtatccca tgcgcatccg tctgcataag acgcagccgc
      241 attttggcga tatcctggga tatcagcgaa atacatgcaa acaccgcatg aaaggagagg
      301 acggcttcag gatcggcttt cacgccctgc tgccaggcgc cggaaaaggg ctcagccacc
      361 gcctgaaaca ggctggtcca gcccgcctct cttacgtcac gtcctgattt ctggtttttt
      421 cgggttcgcc gtaaaaggtt ccacattcgc catgctccgc atcacgtttc tttttctgac
      481 ctgccggacg tcgcaccgtg atgtactccg cctttcccag gcgaaccagc acctccgcac
      541 acggctgtgc cacatcacgg atatccccgg cccgggcatc atgcgtgccc tgcagatatc
      601 ggatctttgc cataacctgt tacgggaggc gcacgcctcc cgtcctcctt atcagactca
      661 gccgccggac gcactgccgt agttcactcc ggtgatcacc gccaccgccg cagtacggcg
      721 acgacgccag ttgatccagc gctccgcacg gatggccacg ctgcctgtct ggaacatgga
      781 aaccagctcc accggggacg gcgtggtact gtcgccgccc ggctcagact gcatttccag
      841 tgatgcctcg cgggacatat ccactgccac gccgccgtca tccgccagat aaatatccgg
      901 ggcattcacc agcaccagct ggtcacccac gtactgggag acaatcaccg gcagcccctg
      961 gaaggagcca cccagcaggg tcatgtccgg gtattccttc tgacccagcg catttttacg
     1021 catggacagt gccagggcat tggtgctgga catcagccag accgcaccgg tgggctgcag
     1081 gtttgctgcc acaaactgtc caaacgcagc ctcggcatcc gcatccgggt taccggttga
     1141 tgccgtgccc ttcacatcat gggtgatgga cgccggggag acatctgcca ctgcggcttt
     1201 tttcgggtcc acaaagtctg tatccagacg cgccaccacc gcttccgcca gcgcattacg
     1261 gaccagtgca tcagcagccg gactggaaaa acggatcaat tcttccgtca gtaccgcaat
     1321 ggccgacacc ttcgcatgac tgaaggtgat ggattcaaaa tcaaacttcg tcaggggttt
     1381 tgccttaccc tcacccaccc agccggcagc accgccggac acctgggcgt gcacacggat
     1441 attgaatggc acctgacgaa gtgcagggat cccgccctga ccaaatcgcc cgataatggt
     1501 ctgcggacgc aggtaatcaa taaagtcctg tgcgtattcc tgatattcag acaggctgcc
     1561 tgcccactgc ggatccgtgg tggtccccgc gcccactgcc gatttcagga catgatgcag
     1621 acgactgtca tccggatact gacgacgggc cacttccagg gcttcagatc ggacgccttt
     1681 agccgcagcc agcgatttgg caaagcgggc gaagccaatc cccttatcca gtttctgctc
     1741 cacacggatc accggcgcag aagccaccgc ggccacattc ccgttaccgg cctgtttcac
     1801 cggctgcgcc gtggcggcct taccggcttc cagttcacgc aggcgcttca ggtgcgcatc
     1861 cacctgacgg atttccgctg cggtgttgtc gtaatgctct tcctcctcca catccagcgt
     1921 gcgcccttcc tctgcggctt tggtcatgac ctcctcaagg gaggctgcca gcgctgcacg
     1981 cttgttttca aaacttttaa tctgttcgcc aatattcatt atggtctttt ccttatgaaa
     2041 aacggttgtt gactgtgccg cagcgccggc agaagatgcg attttcacca ccggtttccg
     2101 gttgccggac gcggcagaaa acgggcggtc gtaagattta atggtccgga tggtgcattc
     2161 cgcattcgcg ggcacggtga cggcagacac ctccatcagt tcccagcgca gaaaatgcag
     2221 tccgcctccg tccagaaagg tgtattcatg gggacggaag cccacggaca gccccctgac
     2281 cagcccggtc ttaatggccg cccagacctc atccagccgg gcagccagtt gcgacggcat
     2341 atccggtacg ggcttcacca gtgttgccgt gatttccagc ccttcgctga cccggcgcac
     2401 cgtacactgc cccaccgggc gggaatggtc atgctgccag agaaacggga tcgcactgcc
     2461 aaactccgcg ccctccggct ccaggatgtc accatcccga tccggagaag gcgttgacgc
     2521 aatcccggtg atcacccgtt catcctcact gaaggatttc accgtcagca gggaacaggc
     2581 ccgtttaaga gtcacatcag cctcctgaaa ataaaaaaac cgccgcagcg gttcatgatg
     2641 gttacagtgt gaacagggtt atatgaaaaa aaccgcatat tctttctttt tcggttccgg
     2701 gttaagggac atcagggaga ccgcattgaa cagcgccatc agcgggtcaa tttttccccg
     2761 tccactggcc tgtttggtga taagaatggc gttaccttta ggctccaccc gggcattgcc
     2821 gacacaccag gccatcaggg gctggtcacc atgcaccagc accccttcag ccagtttgcg
     2881 ctcggtggtt ttaatggccc cgcccagttt ccagccctgg cttatcccca ccacaattcc
     2941 gtcggggatc ccggcttccg ccagtgaatc cagaatctgc cccacccctg acgggtcaat
     3001 accgatatgg tccagtaact cagcctcatg aatgcgacgc acatattccg ccacttccgc
     3061 cgtgtcatcc ccgacacgcc ggacaatggt catatctcca caggcaacaa gatcctgaaa
     3121 ccgggacgcc tcgctcttcc gtcggaccac cgcggtttca tgcgcccagg catggcccca
     3181 gcccagccat tcgcgggttt ccctgtcacg gccaatcacg tacattccca gcagatcatc
     3241 caggcccccg ccgtcaatcc ccaccgtcac cacatcagcg cgctgcagga tatcgtccag
     3301 gctgacgcgc ctgccctgct gctcccagaa atccgcaccc gcccagcggt cagaacgcag
     3361 ggcaagacca atttccacat tggcatgttt tgacatgaag ccacgaaatg cttcctcacc
     3421 agcctcccgg gctttacggt actcccggta cagaaaagcc tcatccaccg aataaccgag
     3481 attcgggtta accatggcga ggttttccat cagcaggtga gccccgcttt ccaccatttc
     3541 aggaggatgc tcaaaaatca ccggcagaaa gtgcggatca tgaattttgc cgtcacggac
     3601 atcccgggcg tactgcagtt tctgtctgaa caccccggct ggcggctcat tcgactgggt
     3661 ggtcgtatac accacaaacc cttccgggcg ggaggcaagg ccgcctatgg cttcacgtaa
     3721 catgtcctcc gctttgtact gcttgccaaa taaccacagt tcatcaatca gcgtccccac
     3781 ggacttgata ccggataccg tattcggatc ggcagccacc accttcaggg tggtgtccgt
     3841 caccctgtgg gtgatggtcc ggatatgtgt ctgcacctga cagaggtcat ccagatcatc
     3901 gtcccgtcgt accatatccc tggcagggtt gaaggcgtta gccgccacct ccacggtcgg
     3961 ggccagaatg gtgtagcccg ccgcctgccg ccagttcagt aacagcgccg tcatcatgat
     4021 cccggcagcc agcgtggact tgctgttttt cttggggata aggataaaaa cttccttgat
     4081 atggcgtaca ccggtctgcg catcgtagga gccaaacagg gccgccacca ggtcaaacac
     4141 ccactgtgcg caggactccc cgaacgtcgg gctgcccggt gcatccacaa tccgcagttg
     4201 tttaaaaatc gccagggcat gtgcggcctg ctccggataa atcggagccg gaataatcga
     4261 cagccccttt ttcaggcgct ctgcccagtc cgggcaggcc gtgctccaca caggtatcat
     4321 ccgttgccct cattatcatt attcaccacc aggcggggtg gtggtggcac cgcaaaacgg
     4381 ttagccgctt ttttcgccgc gtcacctttt gccgattttt taccggcatc cccttttttg
     4441 tggtgcgtga actgcgccag cttataagcc gcatccagcg ccagcctggg gtcggtatta
     4501 atgttctcca ccagaagacg ccccatcgc
//
LOCUS       DAZKFZ010000282         4489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000282 DAZKFZ010000000
VERSION     DAZKFZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            31..390
                     /locus_tag="ACHOQW_004495"
     CDS             31..390
                     /locus_tag="ACHOQW_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9575430.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            399..929
                     /locus_tag="ACHOQW_004496"
     CDS             399..929
                     /locus_tag="ACHOQW_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9575431.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1171..1368
                     /locus_tag="ACHOQW_004497"
     CDS             1171..1368
                     /locus_tag="ACHOQW_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9575432.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1519..2577
                     /locus_tag="ACHOQW_004498"
     CDS             1519..2577
                     /locus_tag="ACHOQW_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9575433.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            2618..2693
                     /locus_tag="ACHOQW_004499"
     tRNA            2618..2693
                     /locus_tag="ACHOQW_004499"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2651..2653,aa:Ile,seq:cat)
     gene            2774..2850
                     /locus_tag="ACHOQW_004500"
     tRNA            2774..2850
                     /locus_tag="ACHOQW_004500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2808..2810,aa:Arg,seq:tct)
     gene            3374..>4489
                     /locus_tag="ACHOQW_004501"
     CDS             3374..>4489
                     /locus_tag="ACHOQW_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9575434.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QVITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADAGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLAQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYGTQYNTIYGAYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDR"
BASE COUNT         1127 a         1012 c         1295 g         1055 t
ORIGIN      
        1 actggcgtgc tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
       61 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc aaaagccggg
      121 gagcgttatc gccgggcagt ggtgcttatt gttcgccagc agcgactgaa attaagcctg
      181 tccggacggc tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg
      241 gacaatattc tgaaagcacc gctggatgcg ctgacgcatg cgggagtgtt aatggacgat
      301 gagcagtttg atgaaatcaa tattgtacgt ggtcagccag tatctggtgg acggctgggt
      361 gtgaagattt acaaaattga gagtgagtga gcgtaaatat gatatatccg gaaattacag
      421 gcaaaagcgg cgaacattta cgcctgaaca cgctggaagc agtctggatc caggggaaat
      481 tacggatgtg ggggcggtgg tcgtatatcg gtgggggtaa atccggaaat atgtttaacc
      541 ggttactggt ttcgaaaaag ctgacgaaaa cagcagttaa tgaggtttta cgcagcatga
      601 agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg accagaggga
      661 agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt gatcgcgtta
      721 tcagtgaggt gcttggggaa tatcccgggc taatcaatat tctccggcaa aggtacgaag
      781 gacggggaat gagtaagaga aaaatggcag aatgtttaaa tcgtactcac ccggaatggt
      841 gtttcagcac atgtgagaaa cgtattgcag gttggttagc cgtggctgaa cacatgcttt
      901 atgtacctat gcacgattca tttcgataaa aaaagcttgc ttttttacgc agaaacagct
      961 tgaattcctg taagcttcgc aaagctgtat cgcgaggcga aatgcaagtt ttttcgcaca
     1021 aggaagccac cggaaggtgg tttttttgtg tccgtaatat acagcagcgc aataaattcg
     1081 ctggtggtta ttaataccgt tctttcaggt tgctggcttt ttcgacaaga gttattggtg
     1141 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gacagaaacc
     1201 gccagagtgg tgtttaatga attaagcgtt accgacccgg cgacagtcgg ggagattgcg
     1261 cagaatactt acctttcacg cgaacgctgc cagttaatac tgacccagct ggttatggcg
     1321 ggtctggcag actatcagtt tggttgttac agacgccttc agtcctgaag gcttttttat
     1381 ttgtggtaaa tgggcggctg gtgggtgtaa ggggcaccca ccagccatct gctcatgcgt
     1441 tgggttcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     1501 agacaggctt aatgatccat gcttaatact gtaaaaatat ccagttgtga gttaatcaac
     1561 gccgactgcc tggaatttat gcggtcgtta cccgaaaatt ctgttgacct gatagtcacg
     1621 gacccgccgt acttcaaagt gaaacccgag ggctgggata accagtgggc gggtgatgaa
     1681 gattacctga agtggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
     1741 ggaagtcttt acctgttctg tggccatcgt ctggcatctg acaccgaaat catgatgcgt
     1801 gagcggttta acgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
     1861 tgcaacaagg aaagcctgcg ggcgtatttc cccgccacag agcgcattct gttcgcagag
     1921 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg taggacactg
     1981 aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc tgtcctgggg
     2041 ataacggcaa aacagattgc agatgccaca ggaaagaaaa acatggtgtc gcactggttc
     2101 agtgccggtc agtggcagct gccgaacgaa agcgattatc tgaaattaca ggcactgttt
     2161 gcccgggtgg cagaagagaa gcatcagcgg ggtgaactgg aaaagcccca ccaccagctg
     2221 gtggatacgt atgcctctct gaaccgacag tatgcggagc tgcagagtga atataagcat
     2281 ctgcggcggt atttcggtgt gacggtgcag gtgccgtaca ccgatgtgtg gacgtataaa
     2341 ccggtgcagt actatccagg gaaacatccg tgcgaaaaac cggcagaaat gttgcagcag
     2401 ataatcagcg caagcagtcg tccgggagac ctggttgcag atttcttcat ggggtcgggg
     2461 tcgacagtga aagcagcgat ggcgctggga cgtcgtgcaa ctggcgttga actggagact
     2521 gaacgttttg agcagacggt gcgggaagta caggatttaa tcattcgtaa cggatgagat
     2581 tgcggagtta atcatgcgtc gttattattc agcaatcggc cctttagctc agcggtgaga
     2641 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca
     2701 ctagctcatc aggaaagaac gtcaccctgt gcgagattcg gagtccccgg tggcggtcca
     2761 ttatcggtat tctgcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
     2821 tcactggttc gaatccagta caacgcacca caccacactt atctgccctg actctctttt
     2881 gcgggctttt tattacagga aagacaccgg acagtgaaat gttaaatgcc tcacaattca
     2941 ggcagttgac tgttgcctga catgctgagc gtttgttaaa aaaatcctgc atgatgaatc
     3001 cccctgagcg gcggggcata atgacagatg tttggttgcg tattgtatag gcaagttgcg
     3061 gattctgtct ggtcattgca gaattcaccg ggaggcaccc ggcatcatgc tgtatacaga
     3121 gattaggcat atatccaggc ttctcatcgc aggagccttt ttacatgcaa aaaaaagccc
     3181 gagtgggttc gggcaacagc atgagatact tgcattgtca tttttatcgt gtggatttta
     3241 accagggttt ataaggctgc gcaactgcgc ggcctttttc gttttgcggg ctgcggttct
     3301 cctcttttga ttctccttgt ggccggaccg tggcccgcaa ctgttgagga aaatcccgga
     3361 aaggggagga ataatgacat ttaaacatta tgatgttgtc agggcggcgt cgccgtcaga
     3421 ccttgcggaa aagctgacac acaaactgaa agagggctgg cagccgtttg gtagtccggt
     3481 ggccataacc ccttataccc tgatgcaggt gattacagca gaaggtgatg tggtggtcag
     3541 tggtgcaact gagccggatt ggtactacgt catcgtactg gccgggcagt ccaatgccat
     3601 ggcttacggt gaagggcttc cgctgccgga ttcatacgat gctccggatc cgcgcattaa
     3661 acagctggcg cgccgcagta cagttacgcc gggtggggct gcctgcagat ataacgatat
     3721 tattccggcc gaccactgcc tgcatgatgt gcaggatatg agtacgctga atcatccgaa
     3781 ggcagacctg agcaaagggc agtacggctg tgtcggccag ggcttacata ttgccaaaaa
     3841 actgcttccg tatatcccga ataacgcggg gatcctgctg gtaccatgct gtcgtggtgg
     3901 ttcggcattc acccagggcg cggaggggac attcagtgcg gacgcggggg ccagccagga
     3961 ttcggcgcgc tggggtgtgg gtaaaccgtt atatcaggac ctgattgcgc gcactaaagc
     4021 tgcattacag aagaacccga aaaatgtgtt gctggcggtg tgctggatgc agggagagtt
     4081 tgacatgagc gccgccaccc acgcacagca acctgcgctg tttacagcca tgctggcaca
     4141 gtttcgtgct gacctctccg tgtttaacgc gcagtgccat ggtggcagtg ctgcagatgt
     4201 gccgtggatt tgtggtgaca cgacgtatta ctggaaaaat acctacggca cccagtacaa
     4261 caccatttac ggggcgtaca aaaacaggga gagtgagggc gtttattttg tgcccttcat
     4321 gacagacggt aacggcgtca ataccgccac taacgcgccg gcagaagatc cggatattcc
     4381 ggcatcagga tattacggtg cggcatcgag aacgaatgga aaccaggtat catcaaaccg
     4441 cccgacacat ttcagttcat gggcgcgcag gagcattatt ccggatcgt
//
LOCUS       DAZKFZ010000283         4488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000283 DAZKFZ010000000
VERSION     DAZKFZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            25..1686
                     /gene="recN"
                     /locus_tag="ACHOQW_004502"
     CDS             25..1686
                     /gene="recN"
                     /locus_tag="ACHOQW_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9575435.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            1835..2176
                     /gene="bamE"
                     /locus_tag="ACHOQW_004503"
     CDS             1835..2176
                     /gene="bamE"
                     /locus_tag="ACHOQW_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9575436.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(2238..2528)
                     /gene="yfjF"
                     /locus_tag="ACHOQW_004504"
     CDS             complement(2238..2528)
                     /gene="yfjF"
                     /locus_tag="ACHOQW_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP9575437.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(2518..2955)
                     /gene="ratA"
                     /locus_tag="ACHOQW_004505"
     CDS             complement(2518..2955)
                     /gene="ratA"
                     /locus_tag="ACHOQW_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP9575438.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            3126..3608
                     /gene="smpB"
                     /locus_tag="ACHOQW_004506"
     CDS             3126..3608
                     /gene="smpB"
                     /locus_tag="ACHOQW_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9575439.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3823..4185
                     /gene="ssrA"
                     /locus_tag="ACHOQW_004507"
     tmRNA           3823..4185
                     /gene="ssrA"
                     /locus_tag="ACHOQW_004507"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1248 a         1151 c         1056 g         1033 t
ORIGIN      
        1 ggtttttcat acaggaaaac gactatgttg gcacaactga ccatcagcaa ctttgctatc
       61 gttcgtgagc ttgagattga ttttcatagc ggcatgaccg taataactgg cgaaaccggt
      121 gcgggtaaat ctattgcaat agatgccctc ggtctttgtc tcggtggtcg cgctgaagcc
      181 gacatggtgc gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc tctgaaagat
      241 acgccagcgg ccctgcgctg gctggaagaa aaccagcttg aagacgggca tgaatgtttg
      301 cttcgtcgcg tgatcagcag cgatggtcgc tcccgtggtt tcatcaacgg tacagctgtt
      361 cctctgtcac aactgcgcga actgggtcag ttgctgattc agatccatgg tcagcacgct
      421 catcagttac tcaccaaacc cgagcaccaa aaattcctgc ttgatggcta tgccaatgaa
      481 acctctctac tccaggaaat gaccgcacgt tatcagttgt ggcatcaaag ctgccgtgac
      541 ctcgcgcatc atcaacagtt aagtcaggaa cgcgctgccc gtgcggaact gctgcaatac
      601 caattaaaag aacttaacga atttaatccg cagcccggag agtttgagca aatcgacgaa
      661 gagtacaaac gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa tgcattggca
      721 ttaatggccg acggtgaaga cgcaaacctg caaagtcagc tttacacggc taaacaactg
      781 gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg tacttgatat gctggaagaa
      841 gctaccatcc agattgttga agccagcgac gaactgcgcc actactgcga tcgtctggat
      901 ctcgatccca accgactgtt tgaacttgaa cagcgcatct caaaacagat ttcgctggca
      961 cgtaaacatc acgtcagccc tgagacattg ccacagtatt accagtcgct actggaagaa
     1021 cagcagcaac tggacgatca ggccgactca caagaaacgc tcgcactggc ggtaacgaaa
     1081 catcatcagc aggcactgga aacggcgcgc gcattacacc agcaacgcca gcattatgca
     1141 gaagaacttg cacagctgat caccgacagt atgcatgcgc tctcaatgcc gcatgggcag
     1201 tttacgattg atgtcaaatt tgacgagcat cacctgggcg ctgacggcgc cgatcgtatt
     1261 gagtttcggg taaccaccaa cccaggtcag ccaatgcagc ctattgccaa agtcgcatcc
     1321 ggtggtgaat tgtcccgcat cgcattggca attcaggtca tcacggcgcg taaaatggaa
     1381 accccggcac tgatttttga tgaagtggat gtagggatta gcggtccaac agcggcagtt
     1441 gtcggcaaac tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt tacccacctg
     1501 ccacaagtcg cgggatgtgg tcatcaacac tattttgtca gcaaagaaac cgatggtgcg
     1561 atgacagaaa cgcatatgca atccctgaat aaaaaaacgc ggttacaaga gctggcgcgc
     1621 ctgcttggtg gcagtgaagt cacacgtaac acactggcga atgcgaaaga actgcttgcg
     1681 gcgtaaactt ttttcctgct tcacggtcag agtaaacagc aaaacgccgt aagaccggaa
     1741 agcaaaaggt tttaaagtga tgaaaggtct attatcatcg gcatattaca gatgagccac
     1801 gtactgctcg ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc tgactgctgc
     1861 agcagcagta ctattgatgt tgaccgcagg ctgttccact ctggagcgag tggtttaccg
     1921 tcctgacatc aaccagggga actatctgac cgctaacgac gtatccaaaa tacgtgttgg
     1981 catgacgcaa caacaagttg cgtacgcatt gggtacaccg ctgatgtccg atccatttgg
     2041 tacgaatacc tggttctatg tcttccgcca gcaaccaggt catgaaggtg taacgcagca
     2101 aacgctgacg ctaaccttta acagtagcgg tgtgttgacc aatattgata acaaacctgc
     2161 gctgagtggt aactaataat aaagttgctc tcaaagacgt taaaaaaggt gctcaatgag
     2221 cacctttttt ctgtccgtta tttattcgct gatttttctg ctcgttgcct gcgaagctct
     2281 ttcggatcgg caatgagagg gcgataaatc tccacccgat cgccatcatg cacactatcg
     2341 cttaattttg ccggacggct gtaaatgccg actttgtttt cagttaaatc gatatcggta
     2401 cgcaattcca gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc ctcctgcagc
     2461 gtcacgcgct gcaggtactg cttcttaggt agcgcataag ccacctcaac ggcaatttta
     2521 cctggcactg taaacctctt tcgcacgaac cgtaaaagcc tggaccatat tagacgccag
     2581 ctctttaaac acgcgaccaa aagcgagttc aatcaactta ttggtaaact caaagtcgag
     2641 atgaaactcg atacgacacg cctcctggct cagcggcgta aacttccatc caccaatcaa
     2701 tttcttgaac ggcccatcca ccagattcat aagaatactt tggttactgg tcaactggtt
     2761 gcgggtagta aacgttttgc tgatcccagc cttagagaca tctaccgccg cagtcatctg
     2821 cccaggagtg gactccagaa tccgacttcc ggtacaaccc ggcaaaaact gaggataaga
     2881 ctgaacgtca ttcactaact gatacatttg ctccgcgctg tagggtacca gtgcggtccg
     2941 gctaatctga ggcataacaa tttccatcaa caaaaatcca acaaataata tcatttatcc
     3001 tgcagtaaaa aaaacgctat cccggcgctg ggtaacatcc ggttcatgct aagatagagc
     3061 cttgtccccc gcaggattga tatggggtat tttcgatttc agattaccga tgattcacga
     3121 cgcttatgac gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg cttaacaagc
     3181 gcgcccgtca cgaatacttt attgaagaag agttcgaagc gggacttgcc ctgcaaggct
     3241 gggaagttaa atccctgcgc gcaggaaaag ccaatattag cgacagctat gtccttctgc
     3301 gcgacggaga agcgttcctg tttggcgcaa acatcacgcc aatggctgtg gcttctacgc
     3361 atgtggtgtg cgatcctacc cgtacccgca agttacttct caaccagcgc gaacttgatt
     3421 cgttgtacgg tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc ctgtactgga
     3481 aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg taagaaacag cacgataaac
     3541 gttcagatat caaagaacgc gaatggcagg tggataaagc gcgtatcatg aaaaacgccc
     3601 accgttaaac ctgcactcca attcttgacc tgttcctcac cgcgcctccc tctccggcgg
     3661 cgcgaatgaa catcttattg gctatcacat ccgacaaaaa tgttgccatc ccattgctta
     3721 atcgaataaa aatcaggcta catgggtgct aaatctttaa cgataacgcc attgaggctg
     3781 gtcatagcgc tcataaatct ggtatacttg ccgttacaca ttggggctga ttctggattc
     3841 gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt aaaaagccgc
     3901 aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc ttaataacct gcttagagcc
     3961 ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat
     4021 cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta atcaggctag tttgttagtg
     4081 gcgtgtccgt ccgcagctgg caagcgaatg taaagactga ctaagcatgt agtaccgagg
     4141 acgtaggaat ttcggacgcg ggttcaactc ccgccagctc caccacttta agaaggacta
     4201 caaccggaca gtagcaataa atacagccac ttacggacac aagccagaca gtagtcggac
     4261 tgaaaaaaga caaaaatata cacgtgaaat gcacgtgcac ttctaaaaaa ccccagttat
     4321 caccgactgg ggtttttcaa tttgtaacta agggtaacaa cccccccgtt tttttcatct
     4381 cactcacctt gatactgttt atttgtacag ttaaaaataa tactgtatac aaacacagta
     4441 tagagggact tttatgcgta ttgagatctg catagccaaa gaaaaaat
//
LOCUS       DAZKFZ010000284         4467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000284 DAZKFZ010000000
VERSION     DAZKFZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            12..1067
                     /gene="sepL"
                     /locus_tag="ACHOQW_004508"
     CDS             12..1067
                     /gene="sepL"
                     /locus_tag="ACHOQW_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFP9575440.1"
                     /translation="MANGIEFNQNPASVFNSNSLDFELESQQLTQKNSSNISSPLINL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIKE
                     LSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYADIISISIKKDKDII
                     LNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHIYYFHLLKDFFTSLPDACFIDSEQRE
                     NALLMIGKVIDYKEEII"
     gene            1126..1704
                     /gene="espA"
                     /locus_tag="ACHOQW_004509"
     CDS             1126..1704
                     /gene="espA"
                     /locus_tag="ACHOQW_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFP9575441.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            1717..2841
                     /gene="espD"
                     /locus_tag="ACHOQW_004510"
     CDS             1717..2841
                     /gene="espD"
                     /locus_tag="ACHOQW_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFP9575442.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            2862..3800
                     /gene="espB"
                     /locus_tag="ACHOQW_004511"
     CDS             2862..3800
                     /gene="espB"
                     /locus_tag="ACHOQW_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFP9575443.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            3807..4214
                     /gene="cesD2"
                     /locus_tag="ACHOQW_004512"
     CDS             3807..4214
                     /gene="cesD2"
                     /locus_tag="ACHOQW_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFP9575444.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            4250..>4467
                     /gene="escF"
                     /locus_tag="ACHOQW_004513"
     CDS             4250..>4467
                     /gene="escF"
                     /locus_tag="ACHOQW_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFP9575445.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRS"
BASE COUNT         1345 a          802 c         1051 g         1269 t
ORIGIN      
        1 gtgagtttcc aatggctaat ggtattgaat ttaatcaaaa ccccgcatct gtttttaatt
       61 ctaattcatt agattttgaa ttagaatctc agcaattaac gcaaaaaaat tcttctaata
      121 tttcttcgcc attaataaat ttgcaaaatg aattagcaat gattactagt tcatcgcttt
      181 ctgaaacgat tgaagggcta agcctgggat atcgcaaagg tagcgcaagg aaagaagagg
      241 aaggctcgac aatcgagaag ctgctcaatg atatgcaaga gctgcttact cttaccgata
      301 gtgataaaat aaaagaacta tcattaaaaa acagcgggct gctggaacaa cacgatccta
      361 ctttggcgat gtttggcaac atgccaaagg gggaaattgt tgcgctaata tcttctttat
      421 tgcaatctaa gtttgttaag attgaattaa aaaagaaata tgccaggtta ttattagatt
      481 tattaggcga agatgattgg gagctggctc tgctttcctg gttaggggtg ggtgagttaa
      541 atcaggaagg tatccagaag atcaagaagc tttatgaaaa ggctaaggat gaggattctg
      601 aaaatggcgc ctctttactt gactggttta tggagattaa ggatcttccc gagcgtgaga
      661 agcacttaaa agtcattatt agggcgctgt cgttcgatct ctcttatatg tcttcttttg
      721 aagacaaagt aaagacatct tcaattatta gtgatttatg cagggtaatc atttttttat
      781 cacttgataa ctatgcagat attatttcga tctctattaa gaaagataaa gatatcattt
      841 taaatgaagt gttatcgatt attgaacatg tctggctaac agaagactgg ttgctggaga
      901 gtccttctcg ggtatcgatt gtcgaagata aacatattta ttattttcat ttattgaaag
      961 acttttttac atcattacca gatgcttgct ttattgatag tgagcagaga gagaatgcat
     1021 tattaatgat tggtaaagtt atcgactata aggaggaaat tatttgatat taattactca
     1081 ataatttttt tgttttcctg agaaaaatta tcaagaggta tatagatgga tacatcaaat
     1141 gcaacatccg ttgttaatgt gagtgcgagt tcttcgacat cgacgatcta tgacttaggt
     1201 aatatgtcga aggatgaggt ggttaagcta tttgaggaac tcggtgtttt tcaggctgcg
     1261 attctcatgt tttcttatat gtatcaggca caaagtaatc tgtcgattgc aaagtttgct
     1321 gatatgaatg aggcatctaa agcgtcaacc acggcacaaa agatggctaa tcttgtggat
     1381 gccaaaattg ctgatgttca gagtagcact gataagaatg cgaaagccaa acttcctcaa
     1441 gacgtgattg actatataaa cgatccacgt aatgacataa gtgtaactgg tattcgtgat
     1501 cttagtggtg atttaagcgc tggtgatctg caaacagtga aggcggctat ttcagctaaa
     1561 gcgaataacc tgacaacggt agtgaataat agccagctcg aaattcagca aatgtcgaat
     1621 acattaaatc tcttaacgag tgcacgttct gatgtgcaat ctctacaata tagaactatt
     1681 tcagcaatat cccttggtaa ataaccggag ataactatgc ttaacgtaaa taacgatacc
     1741 ctgtctgtaa cgtctggggt taataccgcc tcgggtactt ctggtattac tcaatctgaa
     1801 acgggtttat cgctggattt acaactggtt aaatccatga actcgtcagc aggctggaca
     1861 gaaagtagcc ctttaccgac gccgccggca ggtcactcat tagtgacgcc ctctgctgct
     1921 gaggatgtcc ttagtaaatt gtttggtggt attagtggtg aggttacaag tcgcactgag
     1981 gaggcagagc cacagcgcac aagctatccc tatctctctc aggtgaatac cgttgaccct
     2041 cagcaaatga tgatgatggt cactctgtta tccctggata cttccgcgca gaaagtctcg
     2101 agtctgaaaa actctaacga gatttatatg gatgggcaaa ctaaagcgct ggagaataaa
     2161 acgcaggagt ataaaaaaca gctcgaagaa caacagaaag ccgaagagaa atcacaaaaa
     2221 agtaaaattg ttggccaggt ctttggttgg ttgggcgtcg cattaacagc cgttgccgct
     2281 gtttttaacc cagcactctg ggctgttgtt gccattggtg caacagcaat ggcactgcaa
     2341 acggcagtcg atgtaatggg ggaaaatgcc cctcagggat taaagactgc agcacaggtc
     2401 tttggcggaa tatctatggc cgcaagcatt ctgacagccg gcgttggcgg ggtgtcttca
     2461 ctgttatcta aatttggtaa tgttgctaac aaaattggct caagcgttgt aaaagtcgtt
     2521 gagaaggcgg cagaagcgct ggttaaaaac gtttttgcaa aaatttcgac agtggctgag
     2581 ggcgttacga acggtattcg ttctgccggg acaactgcgt tgaataatga ggctgcgcaa
     2641 ctccaaatgt tgtctcagtt agctgctttc gcggtgcaaa acttaactcg acagagtgaa
     2701 agcttaggtg agagtgcgaa gctcgagctg gataaagcgg caagcgagtt acaaaatcag
     2761 gcgagctatt tacaaagtgt ttctcaactg atgtccgatt cagcacgggt aaatagtcgt
     2821 attgttagtg gccgaattta attattaaag agaattttat tatgaatact attgataata
     2881 ctcaagtaac gatggttaat tccgcttcgg agagtacgac cggcgcttcc agtgcagttg
     2941 ccgcatctgc tttatcaatt gattcatctc tgcttactga tggtaaggtt gatatttgta
     3001 agctgatgct ggaaattcaa aaactcctcg gcaagatggt gactctattg caggattacc
     3061 aacaaaaaca attggcgcaa agctatcaga ttcagcaggc cgtttttgag agccagaata
     3121 aagctattga ggaaaaaaaa gccgcggcaa ccgctgcttt ggttggcggg attatttcat
     3181 cagcattggg gatcttaggt tcttttgcag caatgaacaa cgcggctaaa ggggctggtg
     3241 agattgctga aaaagcaagc tctgcatctt caaaggctgc tggtgcggct tctgaggttg
     3301 caaataaagc tctggtcaag gctacggaaa gtgttgctga tgtcgcagag gaggcatcca
     3361 gtgcgatgca gaaagcgatg gccacaacaa cgaaagcagc cagccgtgca tctggcgttg
     3421 cagatgatgt tgcgaaagcc tctgactttg ctgaagatct tgcagacgcc gccgagaaga
     3481 caagcagaat caataagttg ttgaattccg tagataaact gaccaatacc acagcatttg
     3541 ttgccgtgac cagtcttgct gaaggtacga aaacgttgcc aacaacaata tctgagtccg
     3601 tcaaatcgac tcatgaggtt aatgaacaac gtgcgaagtc gctggaaaac ttccagcagg
     3661 ggaatctgga gctgtataaa caagacgttc gcagaacgca ggatgatatc acgactcgtc
     3721 tgcgtgatat aacgtccgct gtccgcgatc tccttgaggt ccagaatcgt atggggcaat
     3781 cgggtcgctt agctgggtaa ttgatcatgg tcgatacgtt taatgatgaa gtgtttaatt
     3841 actatcttga acaaaaaggg tacacaatac agaaggagtt tctttgtggc agtgcctttt
     3901 ttatcggatg gcggattgag acaccttttt tttcattagc gtacagactg gatgaacaag
     3961 aactgatttt gtgctctttt gaagcacgta accaaacagg gcttaacggc cctgttttat
     4021 cgctgactca tttgctcgaa gagttgtatc accatttttc gggtattaag aaaatcagcg
     4081 cgatgaaatc taagattggt tcagattcag aacgtcaaaa gcgcgaagag ttgtttaatt
     4141 acttcatcag aaagggtgcc gtgcagcaag aaacagaaga cggaatttgg ttcgtaatga
     4201 acgtaaatag ttaaaagttt ttattttatt aacttctgag ggaaatttaa tgaatttatc
     4261 tgaaattact caacaaatgg gtgaagtagg taaaacgctg agcgattctg tgccagagtt
     4321 acttaatagc accgatttgg ttaatgaccc tgaaaaaatg ttagagttgc agtttgcggt
     4381 tcagcaatat tctgcttatg ttaacgtaga aagtggaatg ttgaaaacga taaaagatct
     4441 ggtctcaacc atttctaacc gtagttt
//
LOCUS       DAZKFZ010000285         4439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000285 DAZKFZ010000000
VERSION     DAZKFZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            36..398
                     /gene="rusA"
                     /locus_tag="ACHOQW_004514"
     CDS             36..398
                     /gene="rusA"
                     /locus_tag="ACHOQW_004514"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9575446.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            395..535
                     /locus_tag="ACHOQW_004515"
     CDS             395..535
                     /locus_tag="ACHOQW_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9575447.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            621..1055
                     /locus_tag="ACHOQW_004516"
     CDS             621..1055
                     /locus_tag="ACHOQW_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9575448.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1562..2509
                     /gene="stxA1a"
                     /locus_tag="ACHOQW_004517"
     CDS             1562..2509
                     /gene="stxA1a"
                     /locus_tag="ACHOQW_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9575449.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2519..2788
                     /gene="stxB1a"
                     /locus_tag="ACHOQW_004518"
     CDS             2519..2788
                     /gene="stxB1a"
                     /locus_tag="ACHOQW_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9575450.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            3288..>4439
                     /locus_tag="ACHOQW_004519"
     CDS             3288..>4439
                     /locus_tag="ACHOQW_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9575451.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDTEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIIS"
BASE COUNT         1137 a          914 c         1194 g         1194 t
ORIGIN      
        1 caggttatct ggctgaaaga gggggtaatc aaggcgtgaa tacctacagc atcacattac
       61 cctggcctcc gagcaataat cgctattacc gccataatcg cgggcgcacg cacgtcagcg
      121 cagaggggca ggcataccgc gataacgtcg cccgaatcat taaaaacgca atgctggata
      181 tcggcctggc tatgcctgtg aaaatccgca ttgagtgcca catgccggat cgccgtcgcc
      241 gtgacctgga taatctgcaa aaagccgctt ttgacgcact cactaaagca ggtttctggc
      301 tggatgatgc tcaggtcgtt gattaccgcg ttgtgaagat gcctgttacc aaaggtggga
      361 ggctggaact gaccatcacc gaaatgggga atgaatgatg tttgagttta atatggcaga
      421 acttcttcgc caccgctggg ggcgtctgcg cttatatcgt ttccccggtt ctgttttgac
      481 cgattaccga atactgaaga attacgccaa aaccctgaca ggagcaggag tatgaagtca
      541 gagataacaa tcaactaata ctgttttgtt gatttttgct tgtaattggc gttctggtct
      601 gatttttgtg gagtaagttg atgcgtgata ttcagatggt tcttgagcgt tggggagcgt
      661 gggcggctaa taatcatgaa gatgtgacct ggtcgtccat tgccgccggt tttaagggac
      721 tgatccccga aaaagtaaaa tcacgcccgc agtgctgtga cgatgatgcg atggtgatat
      781 gcgggtgtat agcccgcctt taccggaaca atcgcgatct gcatgacttg ctggttgatt
      841 attacgtgtt gggggagacg ttcatggcgc tggcacggaa acatgggtgc tctgacacct
      901 gtataggtaa acgccttcac aaagcggagg ggattgttga aggcatgctg atgatgctgg
      961 gagtgaggct tgagatggat cggtatgttg agcgtgaatt gccgggaggg agaacctctg
     1021 tattttatca gcgaaaaaat agtttacgat cgtaaaaatc tgcatatcat gataagagtg
     1081 gttacattgc cacgctgctt aacccgccga tgcgcgggtt tttttgtacc cagaatcctg
     1141 tgagctatac ggaaagtaca cagaaaggaa ggtgcgacca caattaataa caaaatctta
     1201 aaaattgcac atggcactat tagttttcta aatattgtgt attttttgta ttgcaggatg
     1261 accctgtaac gaagtttgcg taacagcatt ttgctctacg agtttgccag cctcccccag
     1321 tggctggctt ttttatgtcc gtaacatcct gtgtatcaat aaatgttgtt atctacgtac
     1381 gtcaagtagt cgcatgagat ctgaccagat atgttaaggt tgcagctctc tttgaatatg
     1441 attatcattt tcattacgtt attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt
     1501 cagggcgtgg aggatgtcaa gaatatagtt atcgtatggt gctcaaggag tattgtgtaa
     1561 tatgaaaata attattttta gagtgctaac ttttttcttt gttatctttt cagttaatgt
     1621 ggtggcgaag gaatttacct tagacttctc gactgcaaag acgtatgtag attcgctgaa
     1681 tgtcattcgc tctgcaatag gtactccatt acagactatt tcatcaggag gtacgtcttt
     1741 actgatgatt gatagtggca caggggataa tttgtttgca gttgatgtca gagggataga
     1801 tccagaggaa gggcggttta ataatctacg gcttattgtt gaacgaaata atttatatgt
     1861 gacaggattt gttaacagga caaataatgt tttttatcgc tttgctgatt tttcacatgt
     1921 tacctttcca ggtacaacag cggttacatt gtctggtgac agtagctata ccacgttaca
     1981 gcgtgttgca gggatcagtc gtacggggat gcagataaat cgccattcgt tgactacttc
     2041 ttatctggat ttaatgtcgc atagtggaac ctcactgacg cagtctgtgg caagagcgat
     2101 gttacggttt gttactgtga cagctgaagc tttacgtttt cggcaaatac agaggggatt
     2161 tcgtacaaca ctggatgatc tcagtgggcg ttcttatgta atgactgctg aagatgttga
     2221 tcttacattg aactggggaa ggttgagtag cgtcctgcct gactatcatg gacaagactc
     2281 tgttcgtgta ggaagaattt cttttggaag cattaatgca attctgggaa gcgtggcatt
     2341 aatactgaat tgtcatcatc atgcatcgcg agttgccaga atggcatctg atgagtttcc
     2401 ttctatgtgt ccggcagatg gaagagtccg tgggattacg cacaataaaa tattgtggga
     2461 ttcatccact ctgggggcaa ttctgatgcg cagaactatt agcagttgag ggggtaaaat
     2521 gaaaaaaaca ttattaatag ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac
     2581 gcctgattgt gtaactggaa aggtggagta tacaaaatat aatgatgacg atacctttac
     2641 agttaaagtg ggtgataaag aattatttac caacagatgg aatcttcagt ctcttcttct
     2701 cagtgcgcaa attacgggga tgactgtaac cattaaaact aatgcctgtc ataatggagg
     2761 gggattcagc gaagttattt ttcgttgact cagaatagct cagtgaaaat agcaggcgga
     2821 gattcataaa tgttaaatac atctcaattc agtcagttgt tgccggtctg ataatagatg
     2881 tgttagaaaa tttctgcatg gtgaatcccc ctgtgcggag gggcgactgg tgaacggtat
     2941 gatctctttg atgatcgtaa gcgagaatac gcgggtttgg tggcaccagg ccgaactcac
     3001 cgggaggcac ccggcatcat gctgtataca gagattaggc atatatccag gctcctcatc
     3061 gcaggagcct ttttacatgc aaaaaaaagc ccgagtgggt tcgggcaaca gcatgagata
     3121 cttgcattgt catttttatc gtgcggattt taaccaggat tcataaggct gcgcaactgc
     3181 gcggcctttt tcgtatttcg ggctgtagtc cccgtgtgtc attcaggctt ccggactaca
     3241 gcccactcca tatctgattt aatacactat cccggccggg aggaataatg acatttaaac
     3301 attatgatgt tgtcagggcg gcgtcgccgt cagaccttgc ggaaaagctg acacacaaac
     3361 tgaaagaggg ctggcagccg tttggtagtc cggtggccat cacgccttat actctgatgc
     3421 aggccattgc ggcggaaggt gatgtcacca cacctgtgtt ggtgaagccg tcggatggag
     3481 aaggcacagt aatcagcgcc accagagaca cggagtatta ctttgttgtg gttctggcgg
     3541 ggcagtcaaa cagcatggca tatggtgaag gccttccgct gccggagaca tatgaccgtc
     3601 cggacccgcg tattaagcag ctggcgcgcc gcagtacggt gacaccgggc ggtgcagcat
     3661 gcaaatataa cgacatcatt ccggcggacc attgtctgca tgatgtgcag gacatgagcc
     3721 gccttaacca tccgaaagcg gacctgtcaa aggggcagta cggaaccgtg gggcaagggc
     3781 tgcatatcgc caaaaaactg ctgccgttta taccggcgaa tgcgggcatt ctgctggttc
     3841 cgtgctgtcg tggtggttca gcgttcacca ccggagccga tggcacatac agtgacgcga
     3901 gtggtgcctc ggagaattca acccgctggg gtgtggacaa gccgctgtat aaggacctta
     3961 tcggtcgaac aaaagcagca ctgaagaaga acccgaaaaa tgtgctgttt gccgtggtgt
     4021 ggatgcaggg ggaatttgat tttggcggta cgccggtaaa tcacgcagca cagtttggtg
     4081 cgctggttga taaattccgt gcagacctgg cggatatggc aggtcagtgc gtcggtggct
     4141 ctgctggcgg tgttccctgg atatgtggag atacgacgta tttctggaag cagaagaacg
     4201 aatcctcgta ccagacggtg tacggcagct acaaaaacaa aacggaaaag aatatccatt
     4261 tcgtaccgtt catgaccgat gagaacgggg tgaatgtgcc gacgaacaaa ccggaagaag
     4321 acccggacat tccgggtatc ggatattacg gttcgaaatg gcgtgacagc tcagccacct
     4381 ggacgtcaca ggacagggcg agccatttca gttcatgggc tcgccgcggg attatttcc
//
LOCUS       DAZKFZ010000286         4359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000286 DAZKFZ010000000
VERSION     DAZKFZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            488..712
                     /gene="hha"
                     /locus_tag="ACHOQW_004520"
     CDS             488..712
                     /gene="hha"
                     /locus_tag="ACHOQW_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP9575452.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVSSHTRYKLTPAELEA
                     FNSAVDHRLAELIMNKLYDRVPASVWKYVI"
     gene            1347..1916
                     /locus_tag="ACHOQW_004521"
     CDS             1347..1916
                     /locus_tag="ACHOQW_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP9575453.1"
                     /translation="MTYKYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTILHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGCYATLANFPAWPVVWLERGDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            2083..2466
                     /locus_tag="ACHOQW_004522"
     CDS             2083..2466
                     /locus_tag="ACHOQW_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9575454.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPDRREVQLVKRLRQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            2463..2888
                     /locus_tag="ACHOQW_004523"
     CDS             2463..2888
                     /locus_tag="ACHOQW_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9575455.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGRLF
                     PGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGSAST"
BASE COUNT         1067 a         1077 c         1143 g         1072 t
ORIGIN      
        1 cacatgagtt catatatgtg aaataaatct gtgagtgtga tcagcagacc agtctaccca
       61 gtagcaacag attgcgttgt actcacaggg ttatccctga taactggcgt ataacctgcg
      121 tacataaaac gtacctgcaa tgcacctgaa cagagtgcat taagcatccg cctgagcggt
      181 aaacgtatcc gatctggtga gtgacagtct gagagcattc tcagtaatcg cccccttgta
      241 tcctggaaat tggttaagag cgattagatt tggcaaggtg tatgcagcca gcagcttaac
      301 acctcagcta actggctggt taccttcagg ttgtcgcaat ggcatcggat atccggtgtc
      361 ggataactaa acgtagcgtt aaggcgatat tgctgagaga ccggttaagt ggtggcacgg
      421 gagtaatgag tttggggagg gtaccagaat gccagtcagg tgcggtatgc tggcagtatc
      481 acgttgattg cagcataaag taacgagaga cgaatggaag ttaaaaccag agcagttccg
      541 tcgttgttca tcccgggaga cgctggaaaa agtgagttcc cacacacgtt ataaacttac
      601 ccctgcggag ctggaagcct ttaactctgc ggtcgatcac cggctggcag aactgatcat
      661 gaacaaactt tacgatcgcg ttccggcttc tgtctggaaa tatgtcatct gaacgttcgg
      721 taatcacagc ggtattgcgc caggtgttat ctgctctgtg attcttttgc tcttctctgg
      781 ttgctgcaaa gctgaatatg ttcccgttgt tggaagccga ggtaataccg ggaacgttaa
      841 gcggtatagc gacaacaaag aattcgaaat caaaagggaa tggtgtacgg aggtgcgggc
      901 ggtaatgggc cgattttcgg gcattaccga cctctgctga ggttttctcg tatttaccgg
      961 ggggccggtg cgttactgtc tggcacaaca aaggcagcgg gtcgctctgc tccgccgatt
     1021 cctgataatc cctccccagt aacctacggc aacagtacaa atacactgac gcctgaaacg
     1081 gtaaccagaa tcagcaccag cagtaacgtc agtagacaga acagacgatc ggtcttccgg
     1141 taataaccgg aaggatcgac agctccacaa tccgtaacca gtttttacat taaaaaaaca
     1201 acgccggtaa ttcaatcgtc agtccggaga cgaccgttcg ggttatcaca gagtgcatga
     1261 gacattgcct gctggagata acttcagggg ctcttcgcat tttttacagc gtggttattc
     1321 cgtatgaacc atacggagat ttaaccatga cctacaaata caaccccttc tggcaacaac
     1381 gtatccgtga gacggtgctg cgcgcactgg atgttcatcc ccgcctgacg gcacttcggg
     1441 ttgatctgcg tctcccggat gtaccggcag caacggacgc agctgtgata tcccgcttca
     1501 tcaatgccct gaaagcacga atcgacgctt accagaagcg taagcgccgt gaaggtaaac
     1561 gcgtacatcc cacgatcctg cattacgcct gggcccggga attcggggag cttaaaggca
     1621 aaaaacacta tcacctcctg ctgctggtca accgggatac ctggtgtcgt gccggcgatt
     1681 accgcgctcc gggatcactg gccgggatga ttaaacaggc ctggtgcagt gccctggggg
     1741 tggatgccgg atgctatgcc acgctggcga attttccggc ctggccggtg gtgtggctgg
     1801 aacgcggtga tgacaccggt tttcagcagg tgctggagcg tgctgactat ctggcgaagg
     1861 agagtaccaa agcttacggc accggtgagc gcaactttgg ctgcagtcgt ggctgagccc
     1921 gacagacgac ttatctgacc ttagtgtgat gatgcgttca cagcgaccac actgattttc
     1981 ccttctgaga ctgaactgaa aaccgccatt cacacctgat cacgctgccc gtaccgggac
     2041 ggctgttgcc tgcgtgtcgt ctggcgctga ggaacatata ctatgtacgc aaaatccttt
     2101 ctcgctcttg atggcaacgg acgtctgacg ggggcccgta ccgcacagac tgcaccttac
     2161 gaccgctaca cctgccacct ttgcggcagt gcactcagat accacccgca atacgacact
     2221 gagcgtccct ggtttgaaca caccgacgac gggctgactg aacacgctca gcagtgtcct
     2281 tacgtcaggc ctgatcgcag ggaagtgcaa ctggttaaac gtttacggca attcgtaccg
     2341 gatgccttac ccgtagtacg taaagccagc tggtactgca gacaatgtca ccacgattat
     2401 tatggggagc ggtactgcac acactgccag accgggcgct tcagtgaaga ggggggagca
     2461 gaatgaacgg tataaaaacg gggctgggga tcacgccggg tgagcacatt atcagtgccg
     2521 actctgcgct cagcagaaat atccggcatt gtttctgcct ttcctgtcat ggccggttga
     2581 ttctgcagac agatgctcag ggtgcgtggt ttgagcatga tttacatgcc ctgagtgagc
     2641 aacagaaggc ggactgtgtg gtgctgaatc cggaaaagtc ccacccttac attgaggata
     2701 tggcgatgtt tttatcgcca ttaccggtcg tgctggagtg gcattgtgtg atgtgcggga
     2761 ggctttttcc cgggaagaag ttctgtgagg cctgtggtac ggggatttat tgcagagcgg
     2821 tatgtacgaa gtctgtttac agctaccagc cagacttgtt tgaggactgc ggaagtgcga
     2881 gtacttaatc cgaaatcccc gctctgcgca gggcgttcag aggtattgcc aacctgtatt
     2941 ttatccggtg tttcgcaccc accccgttcg tgacacaaag gcgcgacacc ggatatccgc
     3001 agtcctgctt acctgaataa aacccggggt attgattggt agtgtggtgc ggaatcgtac
     3061 cgtgatctcg cattcgccga attcgcactg tggtgtcaat caaccacacg ggggcgtcgt
     3121 ctcagccttt gcctgcctca ggagggtgaa acctgggggg cattgattaa tatcctgccc
     3181 gatctgatga ccggtacttc agacctgcta ccgatacgcc ggaatataac ggtcagagag
     3241 gcatatttcg atgcccctgt tctggagcag ttgatgatgg atgccatagg caatgtaagc
     3301 gctttgtatc accggacgaa gcacgcagta ccagaagccc cccggggttc attggtgacc
     3361 tgacagcgaa gatagattca aagagcgttc agtgattttt cctgcgctcg tcgtggtgta
     3421 cgcaatcagg aatcccgtta tctcattggc atacttgcga taccacacca gcaggacgac
     3481 gctgaaatca cgccgacatt ctcctgtatg gcatcaatct cacaggggac attctgagca
     3541 tgttgacctc aatcacagat cacgacagcg tgctcatttt actgtcttat atcattggtg
     3601 ctgacgcacc ggcccgacaa agggttttac acgctgattc agtgaagacc atttctctaa
     3661 acccatcaaa ctggggtgaa gcaaccgcaa gtacaaaagt gaagtgccac agtagataca
     3721 gcaacgaatc atggaggtca gaagtatgaa acgtgttgtg ataccggtac gctggcaatg
     3781 tgcaaaatgc cagcgctggt attgtggaac ccggccctgc ccttggtgct ggcgatattc
     3841 ccgttcatct tcctgctgat accttccgtt cagcagcggg ttgttatccc ttcctgactg
     3901 attcgtcatt ccattcttat tgattagaac tggcattaca ccggtgccgg cgtgctttcc
     3961 tgcgtgtctg cgccggttca gtaaattaga aaagaaacat ttgtcagcgc cagtgatata
     4021 aggcgtaacg gctaacaatc ttggcgttaa tacattccga gcaaataacc gaagcgttac
     4081 ttttagtgac ggggttatcc aatacttttc tagtcagtgt ttagtattta tgcaagcgat
     4141 ataagtagtc accgcttaga ctattgacat tcatgatgcc tgttaattca aatttcaggt
     4201 atgatcagtc atttattgtt gtgatactga ttccgctagt ttagcataac tgtcctgcaa
     4261 atcattgttc aaaaaagaaa gctcctacaa actgtatcct gtatctctac cagaagataa
     4321 tatacttggt tatttttgtg cataagctca cataaaaat
//
LOCUS       DAZKFZ010000287         4276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000287 DAZKFZ010000000
VERSION     DAZKFZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            47..1705
                     /gene="dpiB"
                     /locus_tag="ACHOQW_004524"
     CDS             47..1705
                     /gene="dpiB"
                     /locus_tag="ACHOQW_004524"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP9575456.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1674..2354
                     /gene="dpiA"
                     /locus_tag="ACHOQW_004525"
                     /gene_synonym="citB"
     CDS             1674..2354
                     /gene="dpiA"
                     /locus_tag="ACHOQW_004525"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP9575457.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2395..3780)
                     /gene="dcuC"
                     /locus_tag="ACHOQW_004526"
     CDS             complement(2395..3780)
                     /gene="dcuC"
                     /locus_tag="ACHOQW_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9575458.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1183 a          984 c         1086 g         1023 t
ORIGIN      
        1 taattacatc cgtcaaagag gctcgggaca acccgcaagg aaaacaatgt tgcagcttaa
       61 cgagaataaa cagtttgcat ttttccaaag actggcattt ccgctgcgta tctttttgct
      121 gattctggtg ttctcaatat ttgtcattgc agccctggcg caatatttta cggccagttt
      181 tgaggactat ttaacgcttc atgtacgcga catggcaatg aatcaggcga aaattattgc
      241 ctccaatgac agtgtcatct ctgaggtgaa aacgcgtgac tacaaacggc tggcgaccat
      301 cgctaacaaa ttacaaagag ataccgattt tgattatgtg gtgattgggg accggcactc
      361 gatccgcctt taccatccta atccggagaa aattggttat cctatgcagt tcaccaaaca
      421 gggcgcgctg gagaaagggg agagctactt cattaccggg aaagggtcaa tggggatggc
      481 gatgcgcgcc aaaacgccaa tctttgatga cgatggaaaa gtcatcggcg tggtgtcgat
      541 tggctacctg gtgagtaaaa tcgatagctg gcgggctgag tttttattac cgatggcagg
      601 cgtgtttgtc gtgctgttag ggattctgat gttgctgtcg tggttcctgg cagcgcatat
      661 ccgtcggcaa atgatgggca tggagccaaa gcaaatcgca cgcgtggtcc gtcagcaaga
      721 ggcgctgttt agttcggttt atgaagggct gattgcggtg gatccgcatg gttacattac
      781 cgccatcaat cgtaacgcaa gaaagatgct ggggctgagc tctcccggac ggcaatggtt
      841 gggtaaaccc attgctgaag tggtcaggcc cgccgatttc tttaccgaac agattgatga
      901 aaaacgtcag gatgtggtgg ctaactttaa cggtctgagc gttattgcca atcgggaagc
      961 tattcgttca ggtgatgatt tgctgggggc cattatcagc tttcgcagta aagacgaaat
     1021 ttccaccctc aatgcgcaac tgacgcaaat aaaacaatac gttgagagcc ttcgtacatt
     1081 gcgacacgag catctcaatt ggatgtcgac gctcaatggt ctgttgcaga tgaaagagta
     1141 tgatcgcgtg ctggcgatgg tgcaggggga gtctcaggcc cagcaacagc ttattgacag
     1201 cctgcgcgag gcgtttgccg atcgccaggt ggcggggctg ctttttggta aagtgcagcg
     1261 cgcccgggaa ctggggctaa aaatgatcat tgtccccggt agccagcttt cgcaactgcc
     1321 gccaggactg gatagcaccg agtttgcagc cattgtgggc aatttacttg ataacgcctt
     1381 cgaagccagc ctgcgtagcg atgaaggaaa caagatcgtt gaattattcc tcagcgatga
     1441 aggcgatgat gtggtgattg aagtcgccga tcagggctgc ggcgttccag agtctctacg
     1501 agacaaaata tttgagcagg gggtcagtac gcgtgctgac gagcccggtg aacatggcat
     1561 tgggttgtac ttgattgcca gctacgtaac gcgctgcggt ggtgttatca ctctcgaaga
     1621 taatgatccc tgcggtacct tattttcaat ctatattccg aaagtgaaac caaatgacag
     1681 ctccattaac cctattgatc gttgaggacg aaacgccgct ggcagagatg catgctgaat
     1741 atattcgtca cattcccgga ttcagtcaga tattactggc gggaaacctg gcgcaggccc
     1801 gaatgatgat cgagcgtttt aagccggggc taatcttgct cgataactat cttcctgacg
     1861 gtagagggat taatttactg catgaactgg tgcaggcgca ttatcccggc gacgtggtgt
     1921 ttaccactgc agccagcgat atggaaacgg tgtctgaagc cgtacgttgt ggtgtatttg
     1981 attatctcat taagcccatt gcctatgaac ggctggggca aacgctaacc cgtttccgcc
     2041 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
     2101 ttaatgccta tgctcgcggt gaacccaaag acgaactgcc gaccggcatt gaccccttga
     2161 cgctaaacgc ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg
     2221 tggcgcaggc actgaccatc agtcgcacca ctgccaggcg ttatcttgaa tattgcgcca
     2281 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagaccg caacgcatat
     2341 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
     2401 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
     2461 caaaccaaca agcaccggta ccgaggtgcg ttttacgact tcaaacggcg agatcttcgc
     2521 catcccggca accgcaacga ctacgccaga aacgggcgaa agggtacggc ccaggtttga
     2581 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
     2641 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
     2701 cagcattgtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     2761 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     2821 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccatcccgc gataagccac
     2881 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     2941 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc
     3001 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg caggggcagt
     3061 ggtggtgatt tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata
     3121 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     3181 cgtcgttttg aaggcgaagt caatcagcga catttcggaa gcttgcgccg ccagcaccac
     3241 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagcagctgc
     3301 gccacgactg ataccaacgt ttaccatcac cggaaatagg gttgccatca gcaaaacacc
     3361 cagaccggtt gcggaagaga cggccagaga cattagacag gcgacaaaat aggcggcaat
     3421 catcagcagg taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat
     3481 atcattcgcg ccgatatggg tcatgtaagc ggcaaatcca cacagcatca taatcatcat
     3541 gccgaggtcg ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc
     3601 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat
     3661 aatcagcaat aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat
     3721 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     3781 aatttttcct gtctccaggc cccaaagtaa ataataaaaa attcctaaag cttaaggaaa
     3841 aaatatgccc aataaattgg cgatgaatgc tgattaaaat caagaaaaac tgccattaag
     3901 acattgaagt tgctgttttt atacgtagat aacaaaacgc atatttagcg gttggcaatt
     3961 atcacataat aatgtgtgta aattgatgga ggattgatgc aaatttgtta attgcaatgg
     4021 tgttagctgc ggttgtgtgc tcaaaaaata atctgcaatg ggcgtgggtt gacgatatat
     4081 aatatattcc ctgttaagat gatttttggt atcttattaa aaaataagtc attttttctt
     4141 ggttgcattt tgttgtcaga aacaactagc tttttctttt atgactattc ccatcgcaga
     4201 aaacgatgtg tcattttaaa tcgatgcgcg gaaatattta acttgaacaa gcggaaataa
     4261 atagagcagc tattca
//
LOCUS       DAZKFZ010000288         4242 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000288 DAZKFZ010000000
VERSION     DAZKFZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..143
                     /locus_tag="ACHOQW_004527"
     CDS             <1..143
                     /locus_tag="ACHOQW_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9575459.1"
                     /translation="GKNETITTPRLSFRFLNVSPTVERQLQRIIFSLEREAREKADKV
                     RD"
     gene            complement(145..756)
                     /gene="emtA"
                     /locus_tag="ACHOQW_004528"
     CDS             complement(145..756)
                     /gene="emtA"
                     /locus_tag="ACHOQW_004528"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP9575460.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            856..1770
                     /gene="ldcA"
                     /locus_tag="ACHOQW_004529"
     CDS             856..1770
                     /gene="ldcA"
                     /locus_tag="ACHOQW_004529"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP9575461.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            1866..3602
                     /gene="cvrA"
                     /locus_tag="ACHOQW_004530"
     CDS             1866..3602
                     /gene="cvrA"
                     /locus_tag="ACHOQW_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP9575462.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(3760..>4242)
                     /locus_tag="ACHOQW_004531"
     CDS             complement(3760..>4242)
                     /locus_tag="ACHOQW_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9575463.1"
                     /translation="FTYVSTWQGFVYVAFIIDVFSGCIVGWRVSSSMETTFVLDALEQ
                     ALWARRPSGTVHHSDKGSQYVSLAYTERLKEAKLLASTGSTGDSYDNAMAESINGLYK
                     AEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHIPPAEAEKAYYASIRNDDLAA
                     "
BASE COUNT          906 a         1083 c         1108 g         1145 t
ORIGIN      
        1 atggcaagaa tgaaaccatc accactcccc gtctgagctt ccgttttctt aacgtcagcc
       61 cgacggtgga gcggcaatta cagcggatta ttttctctct cgagcgggaa gcccgggaga
      121 aagcggacaa agtacgcgac tgatttacat cgcgtccagt gcctgctcaa gtttgtagat
      181 atagcgcgga gcctgcggcg caggatgatt tcgcgctacg tgatcgagga actcgtcagc
      241 atccagatcg ttgattttgc tgattgcctt tttccgatct gacgagaaag tccgtagcag
      301 cgcacctgcc ccgttagcat atgacaccac cagcgcatat tgcagtacct tcggatcttc
      361 aatgcctgcc agcgggccgg tttccagaat attcaggtaa gccgccccca tagaaatatt
      421 acgctccgga tttttcagct cgctggtcgt cggctcacca ctccagccca tacgccgata
      481 aacatcacgt ccggaggttg aagcttttaa ctgcatcaaa ccaatggcat tcgatttact
      541 caccgcgttc ggattaccac ccgattcgat agcgataatc gccgtgatca attgtggatc
      601 gacgccccag gctgcaccgg ctttctggct tattggcatc cactgcatcg cacgttgcac
      661 cggaactttc gcgttccacg gcgggttcgt atagtcatgc tttgatgaac aacccgctaa
      721 taacacaatc aaaaaggcaa accatctcaa tttcacttct tctatcctta tagcctggaa
      781 tcgccgttga ggtgacaacg gcacacctaa gcggcatgat atacatttcg tatctgttgt
      841 cagcaaggaa ttgccatgtc tctgtttcac ttaattgccc catcgggtta ctgcattaaa
      901 cagcacgccg cgctgcgtgg tatccagcgc cttaccgacg cggggcatca agtcaataac
      961 gtcgaggtta ttgcccgtcg ttgtgagcgt tttgcgggta cggaaacgga gcgtctggaa
     1021 gatctcaatt cccttgccag actgactacc cccaacacca tcgtgctggc tgtacgcggc
     1081 ggttacggtg ccagtcgttt actggcagat attgactggc aggcactggt ggctcgccaa
     1141 caatatgacc cgttgctcat atgcgggcat agcgatttta ccgccattca gtgcggtctt
     1201 ctggcgcaag gcaatgtcat cacctttagc ggccccatgc tggtagcaaa ctttggcgcg
     1261 gatgaactga acacctttac cgagcaccat ttctggctgg cgttacgcaa tgaaaccttt
     1321 accattgaat ggcaagggga aggcccgaca tgtcgggccg aaggcacgtt gtggggaggc
     1381 aatcttgcga tgctgatttc actgatcggt acaccgtgga tgccaaaaat tgagaacggt
     1441 attctggtgc tggaagatat taacgagcac cctttccgcg tcgaacgtat gctgttgcag
     1501 ctctatcatg cgggaatttt gccacgtcag aaggcgatta ttctcggtag ctttagcggc
     1561 agtacgccta atgattatga cgcaggttac aacctggagt cagtgtacgc atttttgcgt
     1621 tcccgcctgt cgattccact cattaccggc ctcgattttg gtcatgaaca acgcacggtt
     1681 acgctgccgt tgggcgcaca tgccattctt actaacaccc gggaaggcac tcagttgacg
     1741 attagcggtc atcctgttct taaaatgtaa aaaaaatcac gcttcagggc taagtaaaac
     1801 gctgtctctg ccgctaatat ggtagggttt atataagaga cagcgtaatc aggagtaacc
     1861 gaccgttgga tgccacaaca ataattagcc ttttcatctt aggatccatc cttgttacca
     1921 gcagtatatt acttagttca ttttcttctc gtcttggcat tcctattctg gttatttttc
     1981 tggcgatcgg catgctggcg ggagtcgatg gcgtcggcgg tatcccattt gataattacc
     2041 ccttcgccta catggtaagt aacctggcgc tggcgattat tttgctcgat ggcgggatgc
     2101 gcactcaggc cagttccttt cgtgtggcgt taggaccggc gctgtcgctg gcgacgctgg
     2161 gcgtacttat cacctctggt ttaaccggca tgatggcggc gtggctgttt aatcttgatt
     2221 tgattgaagg cttattaatc ggcgctatcg tcggctccac cgatgctgca gcggtctttt
     2281 ctctgctggg tggtaagggg cttaacgaac gtgttggctc gacgctggaa attgaatccg
     2341 gcagtaatga tccaatggcg gtctttctga cgattaccct gattgcgatg atccagcagc
     2401 atgaaagcag tgtcagttgg atgttcgtgg tcgatattct gcagcaattt ggcctcggga
     2461 ttgtcatcgg tcttggcggt ggttatttac tgctgcaaat gattaatcgc atcgccctgc
     2521 ccgccggttt atatccattg ctggcattaa gcggcggtat tttaattttt gctttaacca
     2581 cggcgctgga aggtagcggt attctggctg tttatctgtg cggttttctg ctgggtaatc
     2641 ggccgattcg caaccgctac ggcatcctgc aaaatttcga cggcctcgcc tggctggcgc
     2701 aaatcgccat gttcctggtg ctggggctgt tggttaaccc aagcgatctg ctgcctattg
     2761 ccattccggc gctcattttg tccgcatgga tgattttctt cgcccgtcct ctttcggtat
     2821 ttgccggatt gctaccattc cgtggcttca atctgcgtga gcgcgtgttt atcagctggg
     2881 tgggattacg cggcgcggtg ccgattattc tggccgtgtt cccgatgatg gcggggctgg
     2941 agaatgcacg tctgttcttt aatgtcgcct tctttgtggt tctggtttca ctgctcttgc
     3001 agggaacatc actctcgtgg gcggcgaaaa aagccaaagt ggtggttccg cccgtcggac
     3061 gtccggtgtc acgcgttggc ctggatattc atccggaaaa tccgtgggag cagtttgttt
     3121 atcaattgag tgccgataaa tggtgcgtgg gcgcggcact gcgcgatttg catatgccaa
     3181 aagagacgcg tattgcggca ctgtttcgtg ataaccagtt gcttcatcct accggcagca
     3241 cccgactgcg cgaaggcgat gtgttgtgtg taattggtcg ggaacgcgat ctcccggcgc
     3301 tcggtaaact gttcagccag tcgccgccgg tcgcgctgga tcaacgcttc tttggtgact
     3361 tcattctcga agccagcgcc aaatatgcag atgtggcgct gatatatggc ctggaagacg
     3421 ggcgagaata tcgtgataag caacaaacgc tgggtgaaat tgtccagcag ttgttaggcg
     3481 cggcaccggt tgtcggtgac caggtagagt ttgccgggat gatctggacg gtggccgaga
     3541 aagaagataa tgaagtgttg aagattggcg ttcgagtggc tgaagaggaa gctgaatctt
     3601 aaatttaatg aagcatctat cgccggttac gatgctttgc ttaacgcagc ctgagcctgt
     3661 atataaactt gtcatttcct gattttgata tgcacattcc aacatcagaa gaaggtttga
     3721 accgccccgg ttttcctgga gagtgtttta tctgtgaact caggctgcca gatcatcgtt
     3781 tctgatggaa gcataataag ctttttctgc ttctgccgga gggatatgac ccagccttcc
     3841 cagcaatcgt cgattgttat accagtccac ccacgtgagt gtggccagtt ccacttctgc
     3901 acggtttttc cagctcttac ggtgtattac ctccgctttg taaagaccat tgatgctctc
     3961 cgccatcgcg ttgtcatacg agtcacctgt actccctgtt gatgccagca gttttgcttc
     4021 ttttagtcgc tccgtatagg ccagtgatac atactgagaa cctttatcac tgtgatggac
     4081 tgtgccggac ggccgacggg cccacaacgc ctgctccagt gcatccagca cgaatgtcgt
     4141 ttccatagac gatgagactc gccaccccac gatacatccg gaaaacacat caatgatgaa
     4201 cgccacatag acgaagccct gccatgtgct gacgtaagta aa
//
LOCUS       DAZKFZ010000289         4217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000289 DAZKFZ010000000
VERSION     DAZKFZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..209)
                     /locus_tag="ACHOQW_004532"
     CDS             complement(<1..209)
                     /locus_tag="ACHOQW_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9575464.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHOQW_004533"
     tRNA            complement(324..399)
                     /locus_tag="ACHOQW_004533"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHOQW_004534"
     tRNA            complement(406..480)
                     /locus_tag="ACHOQW_004534"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHOQW_004535"
     tRNA            complement(597..681)
                     /locus_tag="ACHOQW_004535"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHOQW_004536"
     tRNA            complement(690..765)
                     /locus_tag="ACHOQW_004536"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHOQW_004537"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHOQW_004537"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9575465.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHOQW_004538"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHOQW_004538"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP9575466.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHOQW_004539"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHOQW_004539"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9575467.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1083 a         1047 c          944 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccaa cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAZKFZ010000290         4206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000290 DAZKFZ010000000
VERSION     DAZKFZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            225..833
                     /locus_tag="ACHOQW_004540"
     CDS             225..833
                     /locus_tag="ACHOQW_004540"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP9575468.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            931..2322
                     /gene="selA"
                     /locus_tag="ACHOQW_004541"
     CDS             931..2322
                     /gene="selA"
                     /locus_tag="ACHOQW_004541"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP9575469.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2319..4163
                     /gene="selB"
                     /locus_tag="ACHOQW_004542"
     CDS             2319..4163
                     /gene="selB"
                     /locus_tag="ACHOQW_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP9575470.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          970 a         1067 c         1209 g          960 t
ORIGIN      
        1 tcatccttgt tttatcgggt tatttgtctg cttccaggca aaaccctaca gatttataag
       61 aaagccttaa gtgagatatc catcagatag aaacaattag gaaaatattt atatcaccat
      121 gataaatatg tgatttattt ggaagtaaca aaccatcaac attttctgcc ctttttccca
      181 tcaccaggcc atactgatat ttttcctcgc acaaaggact cggcatgaaa ctcgtcggta
      241 gctacaccag cccgtttgta cgcaaacttt ctattctgtt gttagaaaag ggcataactt
      301 tcgaatttat taatgaactg ccctataacg cggacaacgg cgtgacgcaa tttaacccgt
      361 taggaaaagt gccggtgctg gtgaccgaag agggcgaatg ctggtttgat tcgccgatca
      421 tcgctgaata tattgaatta atgaatgtcg ctccggcgat gttgccgcgc gatccgctgg
      481 agtcgttgcg ggtgcgcaaa attgaggcac tggcggatgg cattatggat gccgggctgg
      541 tatcggtgcg tgaacaggcg cgtccagcgg cgcagcagtc tgaagatgaa ttgttacgcc
      601 agcgggagaa aatcaaccgc agtctggatg tgctggaagg atatctggtc gatggcacac
      661 tcaaaaccga tacggtcaat ctggcgacta tcgccattgc ctgtgccgtc ggatatctca
      721 atttccgccg cgttgcgcca ggttggtgtg tcgaccgccc acacttagtc aaactggtcg
      781 aaaacctgtt tagccgcgaa agttttgctc gcaccgaacc gccaaaggct tgatgcgcga
      841 tatgtcctcc tgacccatct cacgttacaa tccgtggtta tgttaaacgc ccttctccgt
      901 gtgagagggc cttgatcagc caggtttcct atgacaaccg aaacgcgttc cctctatagt
      961 caacttccgg ctattgatcg cttattgcgc gatagctcct tcctttcttt gcgtgatact
     1021 tatggtcaca cccgcgtggt ggaattgttg cgtcagatgc tcgacgaagc gcgagaagtg
     1081 attcgtggca gccagacgct gcctgcgtgg tgtgaaaact gggcgcaaga agtcgatgcc
     1141 cggttgacga aagaagcgca gagcgcgctg cgtccggtga tcaacctgac gggaaccgtg
     1201 ctgcatacca accttgggcg agctttacag gcggaagccg cggtggaagc cgttgcgcag
     1261 gctatgcgtt cgccagtgac cctcgagtac gatctggacg acgccggacg cggacatcgc
     1321 gatcgggcgc tggcgcagct gctgtgccgt attacggggg cggaagatgc ctgtatcgtc
     1381 aataacaatg cggcggcggt gttattgatg ttggcggcca ctgccagcgg aaaagaggtg
     1441 gtggtatctc gcggcgaact ggtggagatt ggcggcgcgt ttcgtattcc cgatgttatg
     1501 cgtcaggcag gctgcaccct acacgaagta gggaccacca accgcacgca cgcgaatgat
     1561 tatcgtcagg cggtgaatga aaataccgca ctgttgatga aagtacatac cagtaactac
     1621 agcattcagg ggttcaccaa agcgatagat gaagcggaac tggtggcgct cggcaaagag
     1681 ctggatgttc ccgtagtgac tgatttaggc agtggctcgc tggtcgatct tagccagtac
     1741 ggtttgccga aagagccaat gccgcaggag ttgattgcgg cgggcgtcag tctggtgagc
     1801 ttctccggcg acaagttgtt aggcgggccg caggcaggga ttatcgttgg taaaaaagag
     1861 atgatcgccc gcctgcaaag ccacccgctg aagcgtgcat tacgcgcgga taaaatgacc
     1921 ctcgcggcgc tggaagccac gttgcgtctt tatttacacc ctgaagctct gagtgaaaaa
     1981 ttaccgaccc tgcgcctgct tacccgcagc gcagaggtca ttcaaatcca ggcacaacgt
     2041 ttacaggctc cccttgccgc acattacggc gcggagtttg cggtacaggt tatgccatgt
     2101 ctttcgcaga ttggcagtgg ttcgctgccg gttgatcgcc tgccgagcgc ggcattaacg
     2161 tttacacccc atgatggacg cggtagccac cttgagtcat tagccgcccg ctggcgtgaa
     2221 ttgccagtgc cggtgattgg tcgtatttat gacggacgat tgtggctgga tttacgctgc
     2281 cttgaagatg agcaacggtt tttggagatg ttgttgaaat gattattgcg actgccggac
     2341 acgttgacca cggcaaaaca accttattgc aggcgattac tggcgtaaat gctgaccgtc
     2401 tgccggaaga aaaaaagcgc ggcatgacca tcgatctcgg ctatgcctac tggccgcagc
     2461 cggatggtcg cgtgcctggt tttatcgacg tacccggtca tgaaaagttt ctttccaaca
     2521 tgctggcggg cgttggtggt atcgatcacg cgctgttggt ggtggcgtgc gatgacggcg
     2581 tgatggcaca gacccgtgag catctggcga ttttgcagct gaccggtaac ccgatgctga
     2641 cagtggcgct gaccaaagcc gatcgcgtag acgaagcgcg tgttgatgag gttgaacgcc
     2701 aggtaaagga ggttctgcgg gaatacggtt ttgctgaggc aaaactgttt atcaccgcag
     2761 caaccgaagg tcggggaatt gatgccctgc gcgagcatct gcttcagttg ccggaacgcg
     2821 agcacgccag ccaacatagt ttccgcctcg cgattgaccg cgcatttacc gtaaaaggtg
     2881 ccgggctggt cgtcaccggt acggcgttaa gcggggaagt gaaggtaggc gattcactct
     2941 ggctgactgg tgtaaataaa ccgatgcgtg tacgtgcgct gcatgcgcaa aaccagccaa
     3001 cagaaaccgc caatgccggg cagcgtatcg cgcttaacat cgcgggtgat gcggaaaaag
     3061 agcagattaa ccgtggcgac tggctgcttg ccgatgtgcc gccagagccg ttcacacggg
     3121 tgattgtcga gcttcaaacc catacaccgc tgacccagtg gcagccgctg catattcacc
     3181 acgccgccag ccacgtcacg ggacgcgttt cactgctgga agataacctt gctgaactgg
     3241 tcttcgaccc cccgttatgg ctggcagata acgaccgcct ggtattgcgc gatatctctg
     3301 cccgcaacac gctggccgga gcgcgcgtcg tgatgcttaa cccgccgcgt cgcggtaaac
     3361 gtaaaccaga gtatctgcaa tgcctggctt ccctcgccag ggcacagaat gatagcgaag
     3421 cgttagccgt tcacctgcaa cgcggcgcgg ttaatcttcc tgatttcgcc tgggcgcgcc
     3481 agctcaacgg cgaagggatg cgcgaactgc tgcaacagcc tggctatatt caggctggtt
     3541 atagcttgtt gaatgcgccg gttgccgctc gctggcaacg gaaaattctc gacacattag
     3601 cgacttatca tgagcaacat cgcgatgaac ctgggcctgg gcgcgaacgt ctgcgacgta
     3661 tggcgttgcc aatggaagat gaagcgctag tactgttgct gattgaaaag atgcgcgaaa
     3721 gcggcgacat cctcagtcat cacggctggc tgcatctgcc ggatcacaaa gcgggcttca
     3781 gcgaagagca gcaggccatc tggcaaaaag cagagccgct gtttggtgac gagccgtggt
     3841 gggtgcgtga tctggcaaaa gagacgggta ccgacgagca ggcaatgcgc ctgacgctac
     3901 gccaggcggc gcagcaagga ataattaccg cgatcgtcaa agatcgttat taccgtaacg
     3961 atcggattgt cgaatttgcc aatatgatcc gcgatctcga tcaggagtgt ggttcaacct
     4021 gcgcggcgga tttccgcgat cgcttaggcg taggccgaaa gctggcaatt cagattctgg
     4081 aatattttga ccgcattggc tttacgcgtc gtcgtggaaa tgatcattta ttgcgcgacg
     4141 cattattatt tccggaaaaa taaggaaatg attaattaat ttttaaaata aattaataca
     4201 aatttc
//
LOCUS       DAZKFZ010000291         4158 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000291 DAZKFZ010000000
VERSION     DAZKFZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..2038
                     /locus_tag="ACHOQW_004543"
     CDS             <1..2038
                     /locus_tag="ACHOQW_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9575471.1"
                     /translation="AGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIH
                     GIARGTAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQTLYV
                     PYSSVPVLQRAGYTRYALAMGEYRSGNNLQSSPRFIQGSLMHGLEGNWTPYGGMQIAE
                     DYQAFNLGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGTNIQV
                     AGYRYSTQGFYNLSDSAYSRMSGYTVKPPTGDSNEQTQFIDYFNLFYSKRGQEQISIS
                     QQLGNYGATFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDH
                     LLAFTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYS
                     VQVGNTHGGNTSSGTSGYSTLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAHADGI
                     TFGQPLGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLA
                     DNVELDETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGS
                     IVAENGQVYLTGLPQSGKLQVSWGNDKNSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            2052..2582
                     /locus_tag="ACHOQW_004544"
     CDS             2052..2582
                     /locus_tag="ACHOQW_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575472.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2595..3098
                     /locus_tag="ACHOQW_004545"
     CDS             2595..3098
                     /locus_tag="ACHOQW_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575473.1"
                     /translation="MKRLHKRFLLATFCALLTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3158..4072
                     /locus_tag="ACHOQW_004546"
     CDS             3158..4072
                     /locus_tag="ACHOQW_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575474.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGTTTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1185 a          896 c          955 g         1122 t
ORIGIN      
        1 tgcaggaaaa tccaactatg catatttgaa tttacagagt ggcatcaaca ttggtagttg
       61 gcgactacgc gataactcaa cgtggagtta taacagtggg agtagcaatt catctgacag
      121 caataaatgg cagcatatca atacgtcggc tgaacgtgac attattccct tacgctcacg
      181 gttaacggta ggtgatagtt ataccgatgg cgatattttt gatagtgtga actttcgtgg
      241 cctcaaaata aattcaacag aagcgatgtt gcccgatagc caacatggtt tcgctccggt
      301 gattcatggt attgcccgtg gcaccgcaca agtgagtgta aaacagaacg gatacgatgt
      361 ttatcagact actgttccac ccggcccttt tactattgat gatatcaact ctgcggccaa
      421 tggtggtgat ctgcaagtaa ccataaaaga ggcagacggc agtattcaga cattatatgt
      481 tccttattcg tctgttccgg ttctccaacg tgctggatat acgcgttatg cgcttgccat
      541 gggggaatat cgtagtggaa ataacctgca aagctccccc aggttcatac aaggtagctt
      601 gatgcatgga ctggaaggaa actggacacc ttatggcgga atgcaaattg cagaagatta
      661 tcaggccttc aaccttggta ttggtaaaga tttaggactt tttggtgcct tttctttcga
      721 tatcacgcag gccaatacga cacttgcaga tggcacccgt cacagcgggc aatcggttaa
      781 atccgtctac agcaaatcct tctaccagac aggaaccaat atccaggtcg caggatatcg
      841 ctattctacg caaggttttt ataacttatc cgacagtgcc tacagtcgaa tgagtggtta
      901 caccgtcaag cctcctaccg gagacagcaa tgagcagaca caatttattg attattttaa
      961 tctgttctac agtaagcgtg gtcaggaaca aataagcatc tctcagcagc ttggaaatta
     1021 cggtgcgaca tttttcagtg ccagtcgcca aagttactgg aacacgtcac gcagcgacca
     1081 gcaaatatca tttggattaa atgtgccgtt tggtgatatt acgacttcgc tgaattacag
     1141 ctattccaat aatatatggc aaaacgatcg ggatcattta ctcgctttta cgcttaatgt
     1201 tcccttcagt cattggatgc gtacagacag tcagtcggca tttcgtaatt caaacgccag
     1261 ttacagtatg tcaaacgatt tgaaaggcgg catgaccaat ctatcggggg tttatggcac
     1321 tctgctgccg gataataacc tgaattatag cgttcaggtc ggtaacaccc acggaggtaa
     1381 tacatcgtct ggcaccagtg gttacagtac tcttaattat cgtggagctt acggcaatac
     1441 taatgtcggt tacagtcgga gtggtgacag cagccagatt tattacggaa tgagtggtgg
     1501 gattattgct catgctgatg gcatcacctt tggacagccg ctgggcgaca caatggttct
     1561 ggttaaggct cctggcgctg ataatgtcaa aatagagaac cagaccggaa ttcataccga
     1621 ctggcgtggc tatgccatat taccatttgc gacagaatat agagaaaatc gtgtcgctct
     1681 taacgcgaat tcccttgcag ataatgttga actggatgaa accgtggtca ctgtcatccc
     1741 aactcacggt gctattgcca gagcaacatt taatgcacaa atcggcggga aagtattaat
     1801 gacgttgaag tacggtaata aaagcgttcc attcggtgca attgtcactc acggagagaa
     1861 taaaaatggc agcattgtcg cggaaaacgg tcaggtttat ctgactggac ttccacagtc
     1921 agggaaatta caggtttcat ggggcaatga taaaaactca aactgtattg tcgattacaa
     1981 gcttcctgaa gtctctcctg gaaccttgct gaaccagcag acagcaatct gtcgctaagg
     2041 aacaatagat tatgaaatac aataacatta ttttcctcgg tttatgtctg gggttaacca
     2101 cctattctgc tttatccgca gatagcgtta ttaaaattag cgggcgcgtc ctcgattatg
     2161 gctgcacagt ctcatcggat tcgcttaatt ttaccgtaga tctccaaaaa aacagtgcca
     2221 gacaatttcc aacgaccggt agcacaagtc cagccgtccc ttttcagatt acgttaagtg
     2281 aatgcagcaa agggacaacg ggggttcggg ttgcatttaa cggtattgag gacgcagaaa
     2341 ataatactct gttgaaactg gatgagggaa gcaatacggc ctccggttta ggtatagaaa
     2401 tactggacgg aaatatgcgt ccggtgaaac tgaatgacct tcatgccggg atgcagtgga
     2461 tcccactggt accagaacag aacaatattt tgccttactc cgctcgtctg aagtcaactc
     2521 agaagtccgt caatccggga ctggtgaggg cttcggcaac ctttaccctt gaatttcaat
     2581 aatcagagtc agatatgaaa agacttcaca agaggttcct cttagctacg ttttgcgcgt
     2641 tattaacagc aactctccag gccgccgatg tcactatcac tgttaatggt cgggtagtcg
     2701 ctaaaccctg cactattcaa accaaagaag ctaacgttaa tctcggggat ctttatacgc
     2761 gcaatctgca acaacctggt tctgcatctg gctggcacaa tattactttg tcattaaccg
     2821 attgtccggc tgaaacaagt gcagtgacgg caatcgtgac aggttcaact gacaatacgg
     2881 gttattacaa aaatgaaggt actgccgaaa atattcagat agagcttagg gatgaccagg
     2941 atgcgacgtt aaaaaatggc gatagcaaaa cggttattgt tgatgagatc actcgtaatg
     3001 cacagtttcc acttaaggca agagctatca cggtgaatgg aaacgcaagc cagggaacga
     3061 tcgaggcgct aatcaatgtg atctacacct ggcaataacg tcgattcgat tttcttagcc
     3121 ttctttttaa atcaattttt taaccttagg atatatcatg ggtaaaacaa tcagtataaa
     3181 ggtcctgttc ggtatttatc ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa
     3241 tgtcgatggc gggtcaagta ttggagctgg tacaacctct gtttatgtta atctcgaccc
     3301 tgtaatacag ccgggccaga atctggttgt agacttgtct cagcatataa gttgctggaa
     3361 tgattacggc ggctggtacg acactgatca tataaacctg gtacaaggct cagctttcgc
     3421 aggctctcta cagtcatata aaggatcgct gtactggaat aatgtgacct accccttccc
     3481 gttgacgaca aataccaacg tattggatat tggtgataag acaccgatgc cattaccgtt
     3541 aaaactctat attaccccag tcggggcggc cggtggagtt gtcataaaag ccggggaagt
     3601 tattgcccgt attcatatgt ataaaattgc cacgttagga agcggaaatc cgcgtaattt
     3661 tacgtggaat attatttcaa ataacagtgt tgttatgcca acaggcggct gcactgttga
     3721 tagtcgtaat gtcaccgtga atctcccgga ctttcctgga agcgcagaaa tcccccttgg
     3781 cgtatattgc tcaagcgaac aaaagctctc attttatctt tcgggtacta ccactgacag
     3841 tgctagacaa gtgtttgcta acacagcacc agatgcaaca aaagcatcgg gagtgggtgt
     3901 ctctctgatg cgtaatggaa aaatactggc tacgggagaa aatgtctccc tggggacagt
     3961 aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag ggaacaaagt
     4021 ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat aattgtgatt
     4081 ttgaccttag tctatgcgct ccattcacga aaattgcgcg gataattatt ttgtgaaggc
     4141 tattatccta cacttgta
//
LOCUS       DAZKFZ010000292         4136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000292 DAZKFZ010000000
VERSION     DAZKFZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..79)
                     /locus_tag="ACHOQW_004547"
     CDS             complement(<1..79)
                     /locus_tag="ACHOQW_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9575475.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTS"
     gene            complement(91..489)
                     /locus_tag="ACHOQW_004548"
     CDS             complement(91..489)
                     /locus_tag="ACHOQW_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9575476.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(531..1556)
                     /locus_tag="ACHOQW_004549"
     CDS             complement(531..1556)
                     /locus_tag="ACHOQW_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9575477.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(1612..1944)
                     /locus_tag="ACHOQW_004550"
     CDS             complement(1612..1944)
                     /locus_tag="ACHOQW_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9575478.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1954..3273)
                     /locus_tag="ACHOQW_004551"
     CDS             complement(1954..3273)
                     /locus_tag="ACHOQW_004551"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9575479.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(3254..>4136)
                     /locus_tag="ACHOQW_004552"
     CDS             complement(3254..>4136)
                     /locus_tag="ACHOQW_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9575480.1"
                     /translation="EDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIES
                     ELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQ
                     TAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRR
                     KFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGL
                     KEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFE
                     SGLRQSTEEEKSDSRAA"
BASE COUNT          814 a         1294 c         1125 g          903 t
ORIGIN      
        1 ctgacgttcc catgtacccg cgtatcgttt catcggcgcg ggcaatggca gcatcgaaca
       61 ggttatcgaa atcagccaca gcgcctcccg ttattgcatt ctggccaggc cgcgctctgt
      121 catttcggct gccacaccgg cagagacacg aaacgccgtt cccggcagca caaatgccac
      181 aggttcatcc cgcgtgacgt gaagtgcatc agtatgcagc ttcaccagtg ccacgaccgt
      241 gaccagttca gacgtatcca gaatcacggt atccggctgc gctgatccca cctcattttc
      301 atgtccggtc agcacatttt cccggctgag aggggtgtcc tgaccggcag tttcatccgt
      361 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
      421 caggctgaca tcacggttca gttgttcacc cagcgagcgg agacgggcaa tcagttcatc
      481 tttcgtcatg gactcctcca cagaaaaaca atggccccga agggccatga ttacgccagt
      541 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
      601 gtgaactcac gcgccggatc gccggtggtc acccagtttt tcgggtaacg ggcagaggcg
      661 ttaatgcctt cgcgctgtgc gtccgcatcc tgaatgcagc cataggtgcg cagaccgcgt
      721 gcctgagtgt tccccagcac catcgtgttg tccggcagga agttcttttt gacgccgttt
      781 tccacgtact gtccggaata cacgacgatg gccacatcgc catacattcc cttataagac
      841 accgctttgc ccaggtcttt taccgctgtc tccagttcgg aatgagagcc gcgacgggta
      901 tccagcttct ccctgacggc tttgaaggaa cggaacagcg cccagccttt cgggtcaaac
      961 acgatgatat tcaccacgcc gctggcgttc agcgcgtagg cttcgatatc gtcggtcggg
     1021 tcatacgtgg acttgtcacg cttgctccac tccgtgccgc cggactgcgt gatgttgttc
     1081 gccgcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
     1141 ttgcccttga gcacggcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
     1201 tcgtcacgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
     1261 tgcggatctt catccggcag gcgacgcagg gtcatctgcg gattcacttc atgcttcggc
     1321 ttgacatatc ccggcgtaaa ttcagaggtg gagccgccac gggaacggat aacctcacca
     1381 gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc ccggaatttg tgagagatag
     1441 actttctccg tggtgaaggg atagctctca cggaaaaaga gacgcagaaa cagcggatca
     1501 aacttaaatt tctgctcatt tgccgccagc agttgggcgg ttgtgtacat cgacataaaa
     1561 aaatcccgta aaaaaagccg cacaggcggc ctttagtgat gaagggtcag gttaaacgat
     1621 gctgattgcc gttccggcaa acgcggtccg ttttttcgtc tcgtcgctgg cagcctccgg
     1681 ccagagcaca tcctcataac ggaacgtgcc ggacttgtag aacgtcagcg tggtgctggt
     1741 ctggtcagca gcaaccgcaa gaatgccaac ggcagcaccg tcggtggtgc catcccacgc
     1801 aaccagctta cggctggagg tgtccagcat cagcggggtc attgcaggcg ctttcgcact
     1861 caatccgccg ggcgcggttg cggtatgagc cgggtcactg ttgcccagcg gctggtaatg
     1921 ggtaaaggtt tctttgctcg tcataaacat cccttacact ggtgtgttca gcaaatcgtt
     1981 aacggcatca gatgccgggt tacctgcagc cagcggtgcc ggtgccccct gcatcagacg
     2041 atccagcgca gtatcactgc gcgcctgtgc actctgtggt gctgcggcca gaatgcggcg
     2101 ggccgtttcc acggtcatac cgggggtttc tgccagcacg cgggcctgtt cttcgcgtcc
     2161 gtgagcctcc tcacagttga ggatccccat aatgcggctg ttttctgccg caaccgcagc
     2221 ggtgatctgc gcgttcacgt ccggctgcgc cgcgctggcg ttttcgccct ccgtcgctgg
     2281 caccacgcca gtaacgtcag cctgcgaagc agtggctgaa acagttgttg attgagtctc
     2341 tttggtcatt cgccctcctg agagacggga tttacgtgca tccagtgcat cacgcataac
     2401 ggtgatcgca tcggtgctgt tgacaagttc atcagccagt ccggcatcaa tggcctcctg
     2461 accgctgtac actgcagcct cggtatccag cacagcctgc acggacaggc cggtatatgc
     2521 cgacaccttc tgcgcaaaca tccggcgggt tgcatccatc cgggactgca gtgtttcccg
     2581 gacatcaccc ggtagatggc tgtaggggtt gccatccacc ttatggctgc cgctgtaaat
     2641 cagcgtgatt tccacgccct gtttctccag ggcagcaccg taattactgt gagccatcat
     2701 gacgccgatg gagcctgtcc gggcggtctg cgtgaccaga cgccgggagg cggcgctggc
     2761 aagcagctga cctgcactgc agttcatgtc gttggccagc gcccataccg gttttatgtc
     2821 tcgcacacgg gcgatgatgt cagcacagtc aaatgctccc gccaccatcc cgcccggtgt
     2881 gtccatatcg agcagaatgc cgtccaccat cggatcgctg gcagcctgtt gcagacgggc
     2941 gataatgccg ttgtaaccgg tcattcccga atacggctgc agcgcccgcg tccggctgac
     3001 cagcgtaccg gacaccggca gcacggcgat gccgttcatg acctgataac tccgggcctg
     3061 tcgtggtccg tcatcatcac cggataacgc cagcgccgcg ggtgcctctc cggcagtcag
     3121 gctgtcgccg gatactgcat ccgtcaggcg gctgatccca agctggcctg caagcgcaca
     3181 aaagaaaacc cgcgcatagg cgggttcaag catcagcggc tcattaaaag ccatgctggc
     3241 aatatgcggg agattacgca gctctgctgt cactcttctc ctcctctgtt gattgtcgca
     3301 gcccggattc aaatgccgca gccgcccagg cgggcggttt aagaccagct gcgcggcgct
     3361 ccatcgtttc acggacctgc tgggcaaaaa tttcctgata gtcgtcaccg cgtttcgcgc
     3421 actctttctc gtaggtgctc agtccggctt ctatcagcat caccgcttcc tgaacttctt
     3481 tcagaccatc gatggccata cgaccggagc ctatccagtc gcagttcccc caggcactgc
     3541 gggcttcctg aaagctgaag cgcgcttttg aaggtaacgt caccacgcgg cgaacgatgg
     3601 cctcttccag ccagcacaga aacatctggc tcgcctgacg ggatgcgacg aattttcgcc
     3661 gccccataaa gtacgcccac gactcgttcg cactggcccg tgccgtggag tagctcatct
     3721 gggcgtaatt ccgggaaagc tgctcatacg agacacccag cccggcagcg atataccgca
     3781 gcagtgactg ctcaaacacg gagtagccgt tatccgtgtc ctgagccgtc tgcaggttca
     3841 gtgagtcccc cggcatcagg tgcggcactt ttgcgcctcc cagacggacc ggtgctgcgg
     3901 cgtaatacgc ggcaatttca ccaatccagc cggtcagcct ttcccgctgc tcctgactgt
     3961 tcgcgcccag aataaaatcc atcgctgact gcgtatccag ctcactctca atggtggcgg
     4021 catacatcgc cttcacaatg gcgctctgca gctgcgtgtt ctgcagcgtg tcgagcatct
     4081 tcatctgctc catcacgctg taaaacacat ttgcaccgcg ggtctgcccg tcctcc
//
LOCUS       DAZKFZ010000293         4106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000293 DAZKFZ010000000
VERSION     DAZKFZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..944
                     /locus_tag="ACHOQW_004553"
     CDS             <1..944
                     /locus_tag="ACHOQW_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP9575481.1"
                     /translation="NQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVN
                     AAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGA
                     LDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQ
                     TDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQ
                     NFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKL
                     PFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1153..2118
                     /gene="lipA"
                     /locus_tag="ACHOQW_004554"
     CDS             1153..2118
                     /gene="lipA"
                     /locus_tag="ACHOQW_004554"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP9575482.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2219..2422)
                     /gene="tatE"
                     /locus_tag="ACHOQW_004555"
     CDS             complement(2219..2422)
                     /gene="tatE"
                     /locus_tag="ACHOQW_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP9575483.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2551..3339)
                     /locus_tag="ACHOQW_004556"
     CDS             complement(2551..3339)
                     /locus_tag="ACHOQW_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP9575484.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3432..3815
                     /gene="crcB"
                     /locus_tag="ACHOQW_004557"
     CDS             3432..3815
                     /gene="crcB"
                     /locus_tag="ACHOQW_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP9575485.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3869..4078)
                     /gene="cspE"
                     /locus_tag="ACHOQW_004558"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3869..4078)
                     /gene="cspE"
                     /locus_tag="ACHOQW_004558"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9575486.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         1073 a         1059 c          938 g         1036 t
ORIGIN      
        1 ataatcaaat tgaatcctgt ttaagcagaa aatcgtcaga aggtaaacca caaatattta
       61 caaccttacg aaatattgat cttaatcttc tgactatttt tgaagctgta tatgtacata
      121 aagggatcgt taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta
      181 ttcagaaact gcgcgttata ttccctgacc cattgtttat tcgcaaaggc cagggtgtca
      241 ctcctaccgc atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc
      301 ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca
      361 ccccctcggt cggagctctg gtccttcctg tcatttatcg ggcgattaaa actcactatc
      421 cgcagctttt actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc
      481 aaaccgatct catcatagat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc
      541 tgttcaccga caatatggtg ttaatttgcc gcgagggaaa tccactactc tctttagaag
      601 atgacagaga gactatcgac aacgctgcac atgtactcct gttaccggaa ggacaaaatt
      661 tcagcggtct gcggcagaga attcaagaga tgtttccgga ccggcagatc agcttcacca
      721 gctacaacat tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc
      781 ccagccgttt ttataacctg tttagccgtt gctggccgct ggaaaaactg ccttttccgt
      841 ccttaaatga ggagcaaata gatttctcta tccactacaa caaattcagc ctgcgtgatc
      901 ccatcctgca cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag
      961 ccagatcaca gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg
     1021 accgctttgg ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa
     1081 tagttgataa ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg
     1141 aacacgcacg ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc
     1201 gataagatgg cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc
     1261 aagccggaat ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa
     1321 gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg
     1381 gcggaatgct tcaaccacgg cacagcaacg tttatgatcc tgggcgctat ttgtacccgc
     1441 cgttgtccgt tctgtgacgt tgcccacggt cgcccggtag ctcctgatgc caatgagcca
     1501 gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt
     1561 gaccgtgatg acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt
     1621 cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgtatg
     1681 gatcgtgctc tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa
     1741 aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag
     1801 ctgctggaac gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg
     1861 ggtctgggtg aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt
     1921 gtgacgatgt taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa
     1981 cgttacgtta gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt
     2041 acccatgctg catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa
     2101 gggatggaag ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct
     2161 ttttgaagcg ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
     2221 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
     2281 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
     2341 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
     2401 tggtaatact aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc
     2461 cttaaaaacc aaagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
     2521 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
     2581 gttgtttaag acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
     2641 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
     2701 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
     2761 atccagcgca cgagcggcaa gcaacgttga ccagtggtgc tctttgagcg gcccgcgaac
     2821 ccaggcggca ggaagtacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc
     2881 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccctcca cctcgagtaa
     2941 cggagcaatt tcattaccag catcaacacg gcgtgattcc tgaatggcaa atgcatcata
     3001 gagatgcaat ttggcataac gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt
     3061 ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg
     3121 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
     3181 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga
     3241 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
     3301 cacagatgta acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg
     3361 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
     3421 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
     3481 tggcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
     3541 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
     3601 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
     3661 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
     3721 gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
     3781 cattctggct gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa
     3841 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
     3901 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
     3961 cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
     4021 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
     4081 aaattacctt tacatgaaaa atagac
//
LOCUS       DAZKFZ010000294         4061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000294 DAZKFZ010000000
VERSION     DAZKFZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..653
                     /gene="hprR"
                     /locus_tag="ACHOQW_004559"
     CDS             <1..653
                     /gene="hprR"
                     /locus_tag="ACHOQW_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP9575487.1"
                     /translation="DNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDDYALIILD
                     IMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLA
                     RVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLASRAGEII
                     PRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            653..2011
                     /gene="hprS"
                     /locus_tag="ACHOQW_004560"
     CDS             653..2011
                     /gene="hprS"
                     /locus_tag="ACHOQW_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP9575488.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDLLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(2119..2970)
                     /gene="hchA"
                     /locus_tag="ACHOQW_004561"
     CDS             complement(2119..2970)
                     /gene="hchA"
                     /locus_tag="ACHOQW_004561"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP9575489.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(3562..>4061)
                     /locus_tag="ACHOQW_004562"
     CDS             complement(3562..>4061)
                     /locus_tag="ACHOQW_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9575490.1"
                     /translation="VHKGTHNTRYGDRFDATAGGETAVAWTVGAKYDANNVYLAAMYA
                     EPRNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQ
                     GNHHYTNKDLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVG
                     LVYQF"
BASE COUNT         1135 a          792 c          984 g         1150 t
ORIGIN      
        1 aagataatca aaggacccag gagtgggtaa cgcaggggct ttccgaagcg ggttatgtca
       61 ttgatgccgt ttctgatggc agagatgggc tttatcttgc gctgaaggat gattatgcat
      121 tgatcattct ggatattatg cttccgggta tggatggctg gcagatctta caaacgttaa
      181 gaacggcaaa gcaaacccct gttatttgcc ttactgcaag ggattctgtc gatgacagag
      241 tcagagggct ggacagtgga gcaaatgatt atctggtaaa acctttttca ttttctgagt
      301 tgctggcaag ggttcgggca caattaaggc aacatcacgc tttgaattca acattagaaa
      361 tcagcggctt aagaatggac tctgttagtc aaagtgtgag cagggacaat atcagtatta
      421 cactgacgcg caaggagttt cagttacttt ggctactggc ctccagagct ggcgaaatta
      481 tacccagaac ggttattgcg agtgaaattt ggggaatcaa ctttgatagt gataccaata
      541 cggtggatgt cgccattcgc aggctccgcg caaaagttga tgatcctttt cctgaaaagc
      601 taattgccac aatccgtggg atgggatatt cgttcgtagc ggtaaaaaaa taatgaaaag
      661 attatctata accgtccgtt taaccttgct ttttatattg ctgctgtctg ttgctggcgc
      721 cggaattgtc tggactctct ataatggcct cgcaagtgag ttgaaatggc gcgatgatac
      781 aacactcatt aaccggacag cgcagatcaa gcagttatta attgatgggg taaatccaga
      841 tacgttacct gtatacttta accggatgat ggatgttagt caggatatct tgatcattca
      901 tggtgatggc atcaataaaa ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa
      961 catacctgct agtgagacaa tcagcgcagc tggcatttac agaagcatta ttaatgatac
     1021 agagatagat gctttacgaa ttaatattga tgaagtttcg ccatcattaa cggttactgt
     1081 ggctaaattg gcttcagcca gacataacat gcttgaacag tataaaatca atagcattat
     1141 aatttgcatt gtcgccatta tactgtgctc agtattaagt ccgctgttaa tccgaacggg
     1201 gttacgagag atcaaaaagt tgagtggtgt aacggaagcg ctgaattata acgatagccg
     1261 ggagcctgtt gaggttagcg cattaccgag agaactaaaa cctcttgggc aggcgttgaa
     1321 taaaatgcat catgctttag tcaaagattt tgagcgtcta agtcagtttg ctgacgatct
     1381 cgctcatgaa cttagaacgc caattaatgc attactgggt cagaatcagg ttacgctcag
     1441 tcaaaccaga agtatcgctg aatatcaaaa aacaattgcc ggtaacattg aagagctgga
     1501 aaatatttcg cggttaacag agaacatact gtttcttgcc agggcagata aaaacaatgt
     1561 tttggtgaaa ctggacttgc tttctctcaa taaggaagtc gaaaatttgt tggactatct
     1621 tgaatatctt tcagacgaga aagagatttg ctttaaggtc gagtgcaatc agcaaatctt
     1681 tgcggataaa attttactgc aacgaatgtt atcgaatctt attgttaacg ctattcgata
     1741 ttcgccagaa aaatcgcgta ttcatataac cagttttctt gataccaaca gctatcttaa
     1801 tattgatatc gccagccctg gagcgaaaat taatgagcct gaaaaactct tccgtagatt
     1861 ttggcgggga gataattcgc gtcattccgt aggtcaggga cttggtcttt ctttagtcaa
     1921 agcgattgcc gaattgcatg ggggaagtgc tacgtatcac tatctcaata agcataatgt
     1981 gttccggatt acgttgccgc aaagaaatta acatagggct tcagtacgcc ataaattaac
     2041 cgtgaaaaaa cgcagccacg tcgtgagtac taacgcggct gcgattgatt atgcgcttac
     2101 attcaaacgt aacagggatt aacccgcgta agctgccagc atttcctgcg ccgccagttt
     2161 acccaacgca ttcgctgcaa aaggactgtc gccggtgaga agtttacggt ccttatgtac
     2221 tcgcccggtg atgtcgtcat taatgatgtt catgcccatt ttcttcagtt cttcgccgaa
     2281 gtaccaggtg agatgacccg gcatatagcc aatctctggc gtttgtttgt ctgcggcgtc
     2341 tgggaatgcg caaatggaat aaccattcag tggattatcg ccgtggcgaa gcgccagaaa
     2401 agccgccggg ccgtggcaaa gggagataac aaaacggtca tttttgattg cccactgtaa
     2461 agcggcagct acgtcctggc tttcaggtaa accaataagt gcgccatgac caccaggaac
     2521 aaagattgct gcatattcgc tatcagcgtt gaggctggcg acaacatccg cgagtttctt
     2581 cggattgcgg aacaacgatt tatgctgctc aaagaatggc attacttttt catctttctg
     2641 cggcatagcc cagtattcaa acttggtcat cagaccggaa atggtcgcca cttcgaattc
     2701 gaaacctgca gcatggagat gatacaacgg cagcaacgtt tcaatcggat ggttaccggt
     2761 cgagaacagt tttccgttat cggtcggcaa ataacgttcg tccgcggcga tcaccagaat
     2821 tttatgttta ccgcgataag gttttggata gtccacgcca tcaagatcag agacaggact
     2881 ggtatattgg ctaagcgaat attctgaagg gaagaatgca ttatcttcag caatatcgac
     2941 ctgcggattt ttactggttt gaacagtcat agtgtattcc ttattgttgc ttagttaggg
     3001 tagtcactat atttgcgact gagctggtcg ctatttttaa ccaggctgga aaattgagtc
     3061 aatatatcga aagcggtagt cggcgggtgt aagtatgtaa tgatgatgct gcactggtgt
     3121 tgaatattat tcacgccaaa ttttccttat tgagcaactt cgtcgcaagt ggcctcaatg
     3181 attttagacc attatcagag gtacggggcg gcggggagag atttagtgct gtggaatatt
     3241 gtggtgctga tgaatattta tatcgcggat tggtagagaa aaagcttatt tcatgcacta
     3301 tttttgcaaa tatttctata tgggaagcgt gtttgttacg gattaattca taaagaaatg
     3361 catttgggtg ttggcttgaa gagtcgctgg ttgatgttat tgtgccagga ggggcagggt
     3421 aagggggtgt atcgataagc tttggcaatt gcgtgtgccg cagatggaga tgaaatgcca
     3481 ataaaaaagc ctgagttcga tgagggacga actcaggcta gtgttcagtt ttacaataac
     3541 acgccgcggc gcgttggctg attagaactg gtagactaag cccacagcca cgatatcatc
     3601 ggtagcgatg ccgttttctt tgtagaagtc gtcgtcgttg tccagcaggt tgattttgta
     3661 atcaacataa gtggacatgt ttttgttgaa gtagtaggtc ataccaatat caacatattt
     3721 aaccagatct ttgttggtgt aatgatgatt accctggctg tcgaagttat cactatttac
     3781 accgcctaaa tccttacctt tagattgcag ataagcgatg gacgggcgca gaccgaagtc
     3841 gaactgatac tgagcaacaa cttcaaagtt ttgggttttg ttagcgatgg catcagcatc
     3901 accgtaacca gtcatgttac ggggttcagc atacatagct gctaagtaaa cgttattagc
     3961 gtcgtattta gcaccaacgg tccatgcaac tgctgtttca ccgccagcag ttgcatcgaa
     4021 gcgatctcca taacgagtgt tatgagtacc tttgtgaacc t
//
LOCUS       DAZKFZ010000295         4060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000295 DAZKFZ010000000
VERSION     DAZKFZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..185
                     /locus_tag="ACHOQW_004563"
     CDS             <1..185
                     /locus_tag="ACHOQW_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9575491.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHOQW_004564"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHOQW_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9575492.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHOQW_004565"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHOQW_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHOQW_004566"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHOQW_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..2738
                     /locus_tag="ACHOQW_004567"
                     /pseudo
     CDS             2431..2738
                     /locus_tag="ACHOQW_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHOQW_004568"
                     /pseudo
     CDS             complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHOQW_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
BASE COUNT         1070 a         1046 c         1082 g          862 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgatgaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacacaatac gatattaaaa aaggcttctc accgatggtc atcgcaacac
     2761 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc tactgccatg atgatccaca
     2821 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     2881 cttctttact gacgcctggc agtttcgcca gcttcgcgga agtatcagag atgcacagca
     2941 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     3001 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttcccgga tgacagtgaa
     3061 actccgtcac ttgcgcgcca tcagtagatg ccgcaggcaa tggaggttcc atcgcaatca
     3121 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     3181 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     3241 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     3301 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggcttac
     3361 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     3421 ttccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     3481 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     3541 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcgccaatca
     3601 aggtatgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     3661 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     3721 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     3781 caaaaaagtt aaaaaatgcc gccatcacca cggcaagctg tggctgcatg gcacgagtat
     3841 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     3901 acacaaatgc cagagcaagt aataacaaaa gccctgtgta tatatcaagg ccaacaaata
     3961 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
     4021 tttaacggtg tgggcaaagt gaaatatcat tttttaacga
//
LOCUS       DAZKFZ010000296         4035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000296 DAZKFZ010000000
VERSION     DAZKFZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.298"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1727
                     /locus_tag="ACHOQW_004569"
     CDS             <1..1727
                     /locus_tag="ACHOQW_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFP9575493.1"
                     /translation="TLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRY
                     SDTVSSLADQWIPLLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVP
                     ANESLVAYLFGAKDGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRH
                     ICGERTARGSTLLASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNW
                     MQAADDASKVTPQDGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIE
                     FIVLSDLFMTPSAKYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDY
                     DWLRDVAKKLGVEAEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKS
                     APHIAFEANIRDPQNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLT
                     AKYPLQLITWKGKNRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDR
                     GVSIIPVEITPRIIPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQT
                     MLVEVEKA"
     gene            1724..2353
                     /locus_tag="ACHOQW_004570"
     CDS             1724..2353
                     /locus_tag="ACHOQW_004570"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFP9575494.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            2346..3167
                     /locus_tag="ACHOQW_004571"
     CDS             2346..3167
                     /locus_tag="ACHOQW_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFP9575495.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            3167..4021
                     /locus_tag="ACHOQW_004572"
     CDS             3167..4021
                     /locus_tag="ACHOQW_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9575496.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
BASE COUNT         1001 a          921 c         1060 g         1053 t
ORIGIN      
        1 atactcttct ggataccaaa ctggtgattc tctgggggca taacccaacg gaaaccatat
       61 tcggtcacac caattactat tttcaaaaaa tgaagcaaaa cggtacacgt tttatcgtgg
      121 tggatccgcg ctattccgac accgtatctt cacttgccga tcagtggatc ccgctgttgc
      181 cgaccacgga caatgcgttg atggacgcga tgatgtacgt cattatcagc gagaatctcc
      241 acgataagac atttatcgac acatatactc tcggttttga tgaaaactcc atgccggaag
      301 gcgtaccagc caacgaatcg ttggttgcct acctttttgg cgcaaaagat gggatacaca
      361 aaacgccgga atgggcagaa aaaattaccc acgttccagc acaatccatt cgtcaactgg
      421 cacgcgatta cgccactaca aaacccgcag cgttgattca gggatggggg ccacagcgac
      481 atatttgcgg cgaacgcacc gcccgaggtt caactttact ggcctcgatt accggtaacg
      541 tcggcatcaa aggtggctgg gcggcaggtt acggcggtag ttctaatcgt aaattctgcg
      601 ttggaccaga tatgccagaa aatccggtac aagcgaaaat ctcgattatg aactggatgc
      661 aggcggcgga tgatgcttct aaagttacgc cgcaagatgg gcttaaaggt gtcgataaac
      721 tcgacagtaa tatccgcctg ctgttctcac tggcaggtaa ttatctggcg aaccagaacc
      781 cggacgtcca ccaggccgcg aagctccttg aagacgaatc taaaatagaa ttcatcgtac
      841 tcagcgatct gttcatgact ccgagcgcga aatatgccga tgtgttgctg ccggaaacca
      901 gctttatgga acgctggaat atcggcgaaa catggggaac tgcgagctat cttattctgt
      961 cagaaaaact gattgagccg gattttgaac gtcgcaccga ttatgactgg ttgcgtgacg
     1021 tggcgaaaaa actgggtgtc gaagccgagt tcagtcaagg acgtgatgag aagcaatgga
     1081 tcgagcatat ctgggagcaa actcgtctgg cgatgccgga tgaaaatttg ccggacttcg
     1141 caaccctgca aaaaacacgt cgacatcttt tcaaaagcgc gccgcacatc gctttcgaag
     1201 caaatattcg cgatccgcag aataatcctt tcccgacgcc ttccggcaaa attgagatct
     1261 tctctaagcg tctttttgat atgcaagatc cagaaattcc ggcgctttcg cactatgtcc
     1321 ctgcctttga agggccagaa gataaactga cggcgaaata cccgttgcag ctcatcacct
     1381 ggaaagggaa aaaccgtgct aattccaccc agtatgctaa cccgtggctg caagaagttc
     1441 agacgcaaaa actctggctg aatccgcaag atgcaaaaca gcgcgggatt agcgaaggag
     1501 atagcgtaaa aatttataac gatcgtggtg tgagcattat cccggtggaa ataacccccc
     1561 gtatcatccc tggcgtagtt gctatgcagg cgggggcatg gtggcaaccg gatgcacaag
     1621 gtatcgatcg cggaggctgt gccaatgtac tgagttcgac ccgcattacg gcgctggcga
     1681 aagggaattc gcatcaaaca atgctggtgg aggttgagaa agcatgagcc aatttaaaga
     1741 atttgcatct gtcagcgaca aacaattagg gttctttatt gactcttcgc gttgttcggg
     1801 ctgtaaagcg tgtcaggtgg cctgtaaaga caaaaacaac cttgaggttg ggcgacgttt
     1861 tcgccgcgtg tatgaagtga acggaggtgg cttcatcgcc actggagagg gcggtgtgag
     1921 tcataatgtc tttgcttata ccctttctgt ttcctgtaac cattgcgcgg atccgatctg
     1981 taccaaaaat tgcccgacga tggcgatgca taagcgtccc ggagacggta ttgtgcgcgt
     2041 gaatactgat aagtgcgtcg gttgcggtta ctgcgcctgg tcctgtcctt acggcgcacc
     2101 acaaatgaat gagcagaccg ggcagatgtc gaaatgcgat ttctgtattg atctgcaagc
     2161 taaaggagaa caacccattt gcgtggcaac gtgcccgttg ggggcgatta agttcggccc
     2221 tattgatgag ttgcgtgaaa agtatggcga tgtcagtgat gtgaaaggtt tacctgattc
     2281 gtcgattacg cagccaaatc tggtgattaa accgcatcag ggcgcggaaa aggaggcgtc
     2341 acatcatgca tgaattaccg ttaatggctt ttacgctgtt gttacaggca tcggtcggta
     2401 tgacgttgtg gctggcgttt cttcatcgtc atattttaat cacctccagc gcccgcccgg
     2461 ttatgcggcc gccattactg ttggcttttt tggcaggtgc cgtagggtta ctgatttcta
     2521 ccttacacct gggttatcca cttaatgcga tgcatgcgct gagccacttt tccagttcat
     2581 ggctgagtcg ggaaattatc tttggtgcgc tgtatctggc gctattgggt ttatccacat
     2641 tactggtcat gttacgcaaa ggcggctggc aactcctgct tatgctagcg gcagtggtcg
     2701 ggattgttga tgttttctgc atggcgcaag tttacatcca tacgtcgatt atcacctggc
     2761 agcattacaa cacgctgatt atgttcttag gtacagtggg aattctcggt tcagcgctgg
     2821 tagcagtgtt tcgtattagc ggaattttgc cgcagattga tgccctgcgt aatggatgcg
     2881 tgttggtgat cgctctcctg gttctgctgc gtttgttggt tcagccgttg tggatagggg
     2941 atttaacggc aaatgcgatg cagatagcca cattgccaca tgcgccatta gcgatgctgg
     3001 gacagttaaa acccatactg acactaagct ggggaatttc cgtcattggg atgatgttct
     3061 ttgctgtcgg tggttgtaaa aagaatatcc cggcggcgtt attcggtagc gtaatgctag
     3121 tggggagtga agttatgctg cgttttgtct tttttagtat cggctaatgt taagtcataa
     3181 cataacgatt ggcgcggaat gcgcgcgcca acgctttcgt tttgcaacat gtcatgcctg
     3241 tgtcgaggct tgcccggcac aggcgctaac gctgacagaa gagggcatag cggtcgacgc
     3301 ggaacaatgt attgattgtg cggtttgcca gtttatttgt ccgcaagagg ctattcgtgg
     3361 cgttaattcc cctttacgtt atttttgcaa tcacaccctg gtcgcacctt tcaccactat
     3421 tgcgccgacg ctggaagaac ttttgctatg gcataaagag caggggatca cagcgatcgc
     3481 gattgatctg agtcaggcga aggcatggga gcgcgcatta ttgctgttaa acgaatgctt
     3541 acggcaatat ggcgaggaag gctggtctgt ggtatctcca cataccagtg aggtgaatgt
     3601 ttcccggcgt cgtttgttcc atgtgcagcg tgcggatgtt cagatgacac aaatacaacc
     3661 aggattacgt cggttacatt atttatggcc ccagattagt gatgctcgcc cgatgatgga
     3721 tcctcaaaag tgtcagcttt gcggtgcatg ctggcgggcc tgcgagcaac aggtcttttc
     3781 gcttaacgaa ggccatttac aaataaatga tgccttgtgt aacggatgcc aaaattgtat
     3841 tgctgtttgt tttcatcagg caatgaccgt tgaattgacg attctaccgg caaaaattgt
     3901 gaatcttcat gctaatcgca aagtctgtaa aacctgtcaa aaatcctttc ttacttttca
     3961 gcaaaatgcg caaaattgtc tttattgcca acgtcatcgt tatgggatga gaacaccata
     4021 aaagtcgagt tgttg
//
LOCUS       DAZKFZ010000297         4034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000297 DAZKFZ010000000
VERSION     DAZKFZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            53..613
                     /locus_tag="ACHOQW_004573"
     CDS             53..613
                     /locus_tag="ACHOQW_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9575497.1"
                     /translation="MISVMILYVMSGSRLADNHTLSAGYAQSKVQDFKNIKGVNLQYR
                     YEWDSPVSVVGSFSYMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFY
                     GLVGISHTKAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNV
                     GYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            749..1540
                     /locus_tag="ACHOQW_004574"
                     /pseudo
     CDS             749..1540
                     /locus_tag="ACHOQW_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1545..2915)
                     /locus_tag="ACHOQW_004575"
                     /pseudo
     CDS             complement(1545..2915)
                     /locus_tag="ACHOQW_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2935..3282)
                     /gene="tnpB"
                     /locus_tag="ACHOQW_004576"
     CDS             complement(2935..3282)
                     /gene="tnpB"
                     /locus_tag="ACHOQW_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9575498.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3279..3953)
                     /gene="tnpA"
                     /locus_tag="ACHOQW_004577"
     CDS             complement(3279..3953)
                     /gene="tnpA"
                     /locus_tag="ACHOQW_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9575499.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          917 a          942 c         1088 g         1087 t
ORIGIN      
        1 attttcagaa atcatttatt aatgaaggag ggttaaatga aaaaaactcg ttataatttc
       61 agtaatgata ttatatgtta tgagtggttc aagactggct gataatcata cgctatcggc
      121 tggttatgct cagagtaagg tacaggactt taagaatatt aaaggagtga atctgcaata
      181 tcgatatgag tgggattctc cggtcagtgt cgtcgggagt ttttcgtata tgaaaggtga
      241 ttgggcggac agtcatcgcg atgaagctga cgatttttac agacatcagg ctgatattaa
      301 atattattca tttctcgcag gcccagctta tcgtctgaat gactatatca gtttttatgg
      361 tttggtgggg atatctcata ctaaagcgaa gggagattat gagtggagaa acagtgtggg
      421 tgctgatgag tctgatggat atttaagtga gtctgtatca aaaaaatcca ctgattttgc
      481 ttatgcagct ggtgttatta ttaatccgtg gggcaatatg tctgtaaatg ttgggtatga
      541 gggaactaaa gcagatatat atggaaaaca ttcggtcaat ggatttactg ttggagttgg
      601 ttatcgtttt taatcattag acgggactgg ataattgcac ttgcgtagtg aagtttacta
      661 aatgtttgta ttttaatgaa gtattcgaca tattcccttc agacggaata tgtcgacggt
      721 tatggtttta ctggagcatg agtatgttat ggcgaaactg ataaatatat cagcgtccac
      781 tgaggagaga atgacgccag gggagaggag ggtggcttca cgactggaga gctttctgaa
      841 tgatgattgc tttgtctggt acgatatccc ggtgggacgg aaaaaccgac atccggattt
      901 tgtcattatt gacccggata acgggctggt atttctggag gtcaaggact ggactgtatc
      961 cacattacgt aaagcaaatc aggagcaggt tacactggaa accgacggtc ttctgaaatc
     1021 agaaataaat ccgctggtac aggttcgtcg ttatgcctgt gatacggtta atgccttacc
     1081 tgctgacccg tgcttacggc agaatgacgg tcagtataag gggcggctga acctggcctg
     1141 ggcgtatggt gtggtgttta cccgcattac ccgtcagcaa ctgaaggcac tgacagggaa
     1201 taatgagaat gcggtggaaa agatttttcc ttcagcacag acaatctgtc aggatgaaat
     1261 gacgcagtct gttttacctg aggtattccg gcaaaaaatt gccgggatgt ttaccacggg
     1321 gttcagaacc cgggtaacac cccggatgag ggatattctg cgcgcccatc tgtttcctga
     1381 agtgaccgtg aaacagaact cacaaatcaa aattatggat attcagcagg agattctggc
     1441 cagaaatatt ggtgacgggc atcgtgtcat tcatggtgtg gccggttccg gaaaaaccat
     1501 gattcttttg ttccgttgcc tttacctggc agaaaccacg tccggtagtt acgccgcccc
     1561 agtgcaacta cccggagggc gttttcacac aggttattgt cgatctccac ccagccattg
     1621 cggcagtatt cgttcagagc gtcccattgc ttcagcagat aggtgaacgc tttcgctgta
     1681 tccgagtggc gcgacagcgt gttcatctgc ccctggagcc actcatacaa cgactgcatt
     1741 agcggtaccg ttctggcttt tctgaccgcc agtcgctctt ctgccggact gccgcggatc
     1801 tcagcctcga tagcgtacag ttccccgata cgacggagcg cttctcccgt tactgtcgtt
     1861 ggatggcgga catgtacatc gtggatcttg cgtcgggcat gtgccataca agccgcttcg
     1921 gttgcctgac cgctttcgta aagagcattg taacccgcat atgcatcggc ctgcaggata
     1981 cctctgtagt ccgccagatg ttgctgtggg tggatgcctt tgcggtcggg agagtatgag
     2041 aaccacaccg ctgccggcat ggtcgaaccg gcgttgcgat cgtcacgaac atagacccac
     2101 agccgtccgg tacgggtttt accctgaccc ggctccagta catttaccgg cgtgtcatcg
     2161 gcatgcactt tacccggcat cagcacatag tgcttcagtt catcatacag cgggcgaagc
     2221 tgctctccca tgatgtcaac ccagcgcccc atcgtattgc ggtgcagctc cacgccctgg
     2281 cgggcataga tttccgactg acggtacagc ggcagatgct cggcgaactt agccatgatt
     2341 atgcgggcca gcagagccgg actggcgtaa ctgcgctcga tgggttttgg tggctgcgga
     2401 gcctgaacta tacagtcgca ccggctgcag gcccgttttg ggcgaaccgt ttcgattacc
     2461 ctgaacgcgg tgttgatgat atccagttgt tcagagatgc tttctcccag cggtttcagt
     2521 ttaccgctgc agacggggca ttcggtttct gccggggaga taacctgcct gtcacgggga
     2581 agtgttgccg gaagtgcttt gcggacggga gagtctgatg ttttcggcgc tgtctctccg
     2641 gccattgagg tgagttgcaa ctgcgcctca ccaagcctgt tctggagctc ggttatacgc
     2701 gtttctgccc gtgcgatctt cttttctatc ttctcgcggc ttttctcgct gctgcgaccg
     2761 aacaacattc tctgcagttt agcgaccagc gctctgagtg agctgatctc gcgggcatag
     2821 ccggttattt caccagacag acggacgata acagcctgct gtgcgatcag cagggccttc
     2881 agttgctcga tgttgtcggg gagtgtgttg ttcattcccc tgttttatca cgggttatat
     2941 ccggatgcca ggccgttctg tccgtttggg atgttgccat gcgatcccct ccagtagcat
     3001 ggataactga gctggcgtca ggtgcacttt cccttcccgg gttaccggcc agacgaagcg
     3061 gccccgttcc aggcgtttgg cgaacaggca taacccgtca cgatcggccc acagtatttt
     3121 caccattttg ccactgcggc cccggaagac gaagatatgc ccggagaacg gggcatcttt
     3181 cagcgtgttc tgcaccttcg aagccaggcc gttgaagcca caacgcatat ctgtgatgcc
     3241 agcgatgatc cagattctgg taccggttgg cagcgttatc atcgggtacc tcctttcatt
     3301 tcgcggatta gcgcccgtaa catttccgga gtgagagggt caaacagttt taccacacct
     3361 gatttaagat gcagctcgca ccgtgggacg tttccgggat cactctcagg gcactcatca
     3421 ggcttgttac gccagaaggg atttgtaact ggtctggtcg gctctggcgt atcagtcaga
     3481 gccaccggga caggcatgca ttcctgtatg tcatcatcgc tcagtaagcc gtcctcgtac
     3541 tggcttttcc attttaacag caggttatca ttgataccgt gctctctggc gatccgggca
     3601 acaacagcac cgggctgtaa tgcctgtttt accagcctga ttttaaattc acgactgtag
     3661 gaggttcgtc gttcttttcg ccatgtgcct tcgctgattt gaggctctgt taattccttc
     3721 tttctgttgg cataaaggat ggcgtcaagc tgagctaatg aaactgaatc gggcaatggc
     3781 catgcgatac cggatgcaat aaatcgctga aaaagcgtat gtattgtgga atgactgaga
     3841 cctagacgct gagcgatggc ccggatggtc agtttatctt caaatcttaa acgcagagca
     3901 tcaggcaaat aagaacggaa gcagggaata tctttttttg tctgggaatt catcgttcgt
     3961 gtccatctat atagatgggc gcgattgttg ccagacagga caattttcac aagacgtcgc
     4021 agatggggcg ctta
//
LOCUS       DAZKFZ010000298         3991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000298 DAZKFZ010000000
VERSION     DAZKFZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..189)
                     /locus_tag="ACHOQW_004578"
     CDS             complement(<1..189)
                     /locus_tag="ACHOQW_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9575500.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLK"
     gene            1013..1786
                     /locus_tag="ACHOQW_004579"
     CDS             1013..1786
                     /locus_tag="ACHOQW_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575501.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAAADDDDLLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASKAEVILATVRGIVRGVFIADKWLKSTREHFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(1844..2398)
                     /gene="pinE"
                     /locus_tag="ACHOQW_004580"
     CDS             complement(1844..2398)
                     /gene="pinE"
                     /locus_tag="ACHOQW_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFP9575502.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
                     RQKVAIIYDVGVSTLYKKFPAGDK"
     gene            2461..2922
                     /locus_tag="ACHOQW_004581"
                     /pseudo
     CDS             2461..2922
                     /locus_tag="ACHOQW_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2922..3515
                     /locus_tag="ACHOQW_004582"
     CDS             2922..3515
                     /locus_tag="ACHOQW_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9575503.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGRVAVFTGKGWSHEEDHRNETVYSIENGAAVTVDYIGAIKDGYVT
                     ISPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(3487..3930)
                     /locus_tag="ACHOQW_004583"
     CDS             complement(3487..3930)
                     /locus_tag="ACHOQW_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFP9575504.1"
                     /translation="MDEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGDELTQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
BASE COUNT         1153 a          910 c          868 g         1060 t
ORIGIN      
        1 tttcaaatag cgactaataa actcagcgcc aaaatccgcc actatcactc tttcaggata
       61 tgtcaggaaa aaatctctag agctagagtc gacaagtaca gatgaacctc tctccagaaa
      121 cacactctct tttccgaaat aaacatcaaa ggactccaaa atcaatataa ctgaacatgt
      181 agttgccata tcatcccccc aatttaactg aaaccaggat gaagtatatg tatataaagt
      241 tcactttgcc aaacttcagt ataaaaaacc actaaaaata gggtgtgata aaaaataccg
      301 taacaataaa gcaaaggatt ataaattccg ttacagttac aaatgatact caagaaacaa
      361 ttccttagga aaaacttatt tacagccaat aagtaagaca cttatatgat atcaagtttt
      421 cataaaacat aactaggcta aatagctgcg cctaataccg ctacactttt gccagcccat
      481 gtttgcctcc gggtattgac cccttctcta cgcaacttca gttcccacca ccaactttgc
      541 ggcagctttg taggatcaat gtctaaaaga ataatggtga ccgataagaa aacgactgaa
      601 taactgcaga ttttcgctca aaaccttcct gtcagatcca tagcgaatca agtgctgaat
      661 gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatt ctacgattgt
      721 tctgtttagg aaaagcaaga cgggaagtcg ggagataagt cattgataaa gtggcggaga
      781 gagggggatt tgaacccccg gtagagttgc ccctactccg gttttcgaga ccgacccgat
      841 catcaaacga aacataaaat tagctcacat tatgaggaaa ggtatctttt tgcgctatgt
      901 aaattcaaag ggttagcctc attttcccga tggctttctc aacactacta gttgtgagcc
      961 cttgcaatgt tcattaatat acgtctcaca aataattcac agatattgca aaatggatat
     1021 tactgagttt ccttctggag taattgaaca ccttggctgg tatgtatacc gattgattga
     1081 tcctagggac ggaagcacct tctatgtagg gaaaggcaaa ggtaaccgcg tatttgccca
     1141 tatgcgcggt gaagtggcag cggctgatga tgacgactta ctgagcaaca agctaaagca
     1201 aatcagagaa ataagattag cgggacttga agttatccat gtcatccatc gacatggaat
     1261 gactgatgaa aagacggcgt acgaagttga agcagcactt attgatgcct accctgggtt
     1321 aacgaatatc atgaatggtg ctggcagcaa tgaattcggc gccgcgcatg tcaaagagtt
     1381 gatagcaaca tatcaacccg aaaccataac atttcatcat aaagcattaa tgatttccgt
     1441 taacagaagt gcaaaagatt cagagcttta tgatgcggtt cgatttagct ggcgcattaa
     1501 tgtctcgcgc gccagtaaag cagaagtcat tcttgctact gtaaggggga tcgttcgagg
     1561 agttttcatt gctgataaat ggctaaaatc aacgcgtgaa cattttccta cgatgaaata
     1621 ctgggacgaa gaccctgact ttgaggcaac acaaagctct cgttatggtt ttgaaggtcg
     1681 agaagcccca cctgaaatag caaaccttta tcttgggaaa aaaataccag atgaattaag
     1741 aaaaaaagga gctatgtctc cagttcgtta ctcacctaat ttttgaatct tcaagtggta
     1801 agcataaacc gcagcacgat cttgcatacg acgtgctacg gtttcattta tctccggccg
     1861 gaaacttctt atacagtgtc gatacaccaa catcatagat gatcgctacc ttctggcgag
     1921 gaattcctgc tgcaattaat cgtccggcct gcgcccattg ttctggtgta agtttgggac
     1981 gacgtccacc aattcgtccc tgtgcgcgag cagcttccag tccagctttt gttcgttcaa
     2041 caatcagttc tcgttccatt tcagccaggg cccccatcac atgaaagaaa aagcgcccca
     2101 ttggggtact ggtatcaata gcatccgtca ggctgcgaaa attaacgcca cgttcgcgca
     2161 actcctcaac cagaatgacc agatgccgca tactgcgccc cagccgatcc agtttccaga
     2221 caaccagagt gtcacctgcc gataatgtcc tgagcagttt tttcagtccc ggcctttcgg
     2281 actttgtacc gcttatcttg tcttcaaaaa tcagctcgca tcctgcacag ttcagcgcat
     2341 tacgttgtag atctgtgttc tggtcatttg ttgacacacg tacatagcca ataagcatgg
     2401 tagatctccc tgacaaaagc aggaatgatg ccatttgctc gttatttctg cattttcata
     2461 aacgttggtt tgggagaagg tgctccagct attggcgttc cgttcttctg gccgtccgct
     2521 gcaatgccaa atactgtaat cgacagctgg tcctgtatgg tgtttttgaa gttcaacggc
     2581 gcgaaattct ctgccactga ttatcctgtg ctggcgaaag tgtttccttc gctggtatta
     2641 cctgaagccc gcggtgattt cattcgtatc tgggatgacg ggcgaggtgc agacggtggt
     2701 cgcgaattat taagctggca ggcagctaca aacttttctc agtttgccgg gaatataggc
     2761 gaaggtgcgg gacacgcaat taactttcat gatggcatcg ccggaaatca gccaggattt
     2821 tcacgattta atttcaccag taactctgtg ggtgatggtg tgaattttgt tgcagtcaga
     2881 ccgcgaaata ttgcatttaa ctttctggtg agggctaaat aatgaaacct gtttttgatg
     2941 aaaatgggct ggctacagtg ccgggcgata tgcgttgttt ttattatgat gctgaaacat
     3001 ctgagtatac gggctggtct gatgaatata ttaatactgg cgtaagtatg cccgcctgtt
     3061 ccactggtat tgaccctggc gaaaacattc cgggaagagt ggcagtattt acaggtaagg
     3121 gatggagcca tgaagaagac catcgcaatg agactgttta ctcaatcgaa aatggcgcag
     3181 ctgttacagt ggattatatc ggtgccatca aagacggtta tgtcacgatt tcaccgttaa
     3241 cgccatacga taaatgggat ggtgagaaat gggtgacaga cactgaggca caacacagtg
     3301 ccgcagtaga cgcggcagaa gcacagcgcc aatcactgat tgatgcagca atggcttcca
     3361 ttagtctgat tcagctgaag ttacaggccg gacggaaact gactcaggca gaaacaaccc
     3421 gacttaacgc cgtgctggat tacattgacg cggtgacggc aacagatacc agcaccgcgc
     3481 cggatgtcat ctggcctgaa ctgccggagg cgtaggccat tcaatatctg gcgcaccgga
     3541 agtatcgacc agctccagtg cgtccagata atccagccac aaattatatt gcgtcagttc
     3601 gtcacctttc agacgaccaa tagccgcttt accgggccat tgcttactgt tcatgtattc
     3661 gttggcctgg ttaattagta gctgtctttc tgattcagta atttcaataa gctcttcatg
     3721 cgtgggtgga ggaatatctg cccacgcagg cagcccatca tctccggcaa tacggatttt
     3781 tccttgtggc ggttcagcca taaactcact gataatattt tgatttactt ccttagcgtc
     3841 tgataaatcc catccctctg atttatattt atcaatcata tccacaggga aaaaagcatt
     3901 atgccttgcg ctataaacat attcgtccat ataaatcacc ctgaataaaa ttactcacca
     3961 acagcccacc aactgtaatt catcgatacc g
//
LOCUS       DAZKFZ010000299         3896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000299 DAZKFZ010000000
VERSION     DAZKFZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..837
                     /locus_tag="ACHOQW_004584"
     CDS             <1..837
                     /locus_tag="ACHOQW_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575505.1"
                     /translation="KNIQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKSSNAQAVLP
                     YVVKIARGTPDFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKFNWCVNYLT
                     SKLAGDTFTLGLNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANSKEGTITLK
                     CDNLFGDKKQASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPPKGTEAAIK
                     ISANGDQGAATSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALEATALINVK
                     YD"
     gene            complement(919..1257)
                     /gene="rcnB"
                     /locus_tag="ACHOQW_004585"
     CDS             complement(919..1257)
                     /gene="rcnB"
                     /locus_tag="ACHOQW_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP9575506.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1475..2332)
                     /locus_tag="ACHOQW_004586"
     CDS             complement(1475..2332)
                     /locus_tag="ACHOQW_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP9575507.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHDHEHHHHHEHGDNEEY
                     QDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGA
                     TLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMG
                     VHGFMGIMR"
     gene            2453..2725
                     /gene="rcnR"
                     /locus_tag="ACHOQW_004587"
     CDS             2453..2725
                     /gene="rcnR"
                     /locus_tag="ACHOQW_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP9575508.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(2835..3149)
                     /locus_tag="ACHOQW_004588"
     CDS             complement(2835..3149)
                     /locus_tag="ACHOQW_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFP9575509.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(3159..3506)
                     /locus_tag="ACHOQW_004589"
     CDS             complement(3159..3506)
                     /locus_tag="ACHOQW_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575510.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         1112 a          787 c          885 g         1112 t
ORIGIN      
        1 aaaaatatcc aaagtgattg ggttgaagtt gggccttttg gtaatggcga aaaattaaag
       61 gttaaaatag agtctttagg taaaaccagc gacacaattg ggaaatccag caatgcgcag
      121 gcagtattac cttatgtggt taaaatagcc agaggcacac ctgattttac tggagaaaga
      181 aaatctacct ggtttatttc agataccgtg attgcaaata ttggcggtga gtcatcgtca
      241 tccatcgatt tttggttggg tatttgtaag gcattgaagt ttaactggtg tgtgaattat
      301 ctcaccagca aactggcggg ggatacattt acgcttgggt taaatatttc ctattatcct
      361 aaaaatacga cctgtaagcc tgaaaacacc gttataaaag tagatgatat cgccttgttc
      421 cagctcagaa atcagggaaa gattgcggcg aacagtaagg aaggaacaat tacgttgaaa
      481 tgtgataatc ttttcggcga caaaaaacaa gcatcgcgga atatggttgt atatctttct
      541 agcagtgact tagttaaagg aagtaatact attttgcgtg gtaaaacaga taatggtgta
      601 gggtttgtgt tggatctaac agaaccacca aaagggactg aggctgccat taaaatttcg
      661 gccaacggcg atcagggcgc ggcgacatca ttatggaaaa cagataaacc aggagtttca
      721 ttaaatagca acattattaa tataccagtc atggccagtt actatgtata tgatgaaaaa
      781 aaagttaaat ctggcgcact ggaagcaacc gcattaatca acgtgaaata cgattaataa
      841 caagcaagat gagcaagtgg ctaataataa gaaataataa ggtgtctgca tttccccctc
      901 tctccgaggg ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat
      961 tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc
     1021 ggcatccggc gcgggcaggt tacgcaactg ccactgctta atgttgtact catcggtacg
     1081 gtacatctct ggtacggtgt cccctggttt gtaatgctta aagtcagcaa tgaaactgct
     1141 taactcatac ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca
     1201 ggcggcactg gtaaccagca aaagcgcacc cagcaacatc ttatttttaa tggtcataaa
     1261 ctctccactt tggggatctg taatatcagt cgcatatctt tcctgccata caatataact
     1321 gacaagcgat attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgt
     1381 agcaaaagca tcctcattga gcgagaagtg agcggaataa gctgatttaa gaatcatcct
     1441 gggctaaaag gatagcgaag cccaggtgat tgttttatct cataataccc ataaagccat
     1501 gcactcccat atacaccccg actaaaccaa ttaacagact ggaaaaatag ggggcgcgtt
     1561 tagcgagagt gttaaatccg ctccagcgtt ttgcaacttg ttgaacactg attgccgctc
     1621 caacgcctac tgtaaccagc gttagtgcca gaccgatgct gaaactgacg accagtgttg
     1681 cgcccagtgt cagggctttc aactgaatgc aaatcaacag cacggtaatt gctgccgggc
     1741 agggaataag gccacctgtt aagccaaata acaaaatttg ccagttggtg acctctctac
     1801 catcaaagcg tcgtttaatg tcattggcat gggctcgtgc atgggcatcc tgatactctt
     1861 cgttgtcgcc atgttcatga tggtgatgat gttcgtggtc gtggtcgtgg tcgtggtcgt
     1921 ggtcgtggtc gtggtcttga tgatgttcgt gatcgtggtg atgatgctca tgcatattct
     1981 ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaaagcg gtgctaataa
     2041 tgatcactgc ggaaatcaac tggagccacg gttctgctga ttgagcagta aagcgcttgc
     2101 tgatcaccat cccgccaaag gcaatcaacc agaccactgc ggtatgcgaa atggttgctg
     2161 ccagtccgag catcaccgcc tgtttaatgg tgcctttgat ggcgatgata aacgccgcca
     2221 tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag atggcgctgg
     2281 ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc atgataataa
     2341 ttcttagtat taattcggca atctgattct actccccccc agtacctgat actacccccc
     2401 agtagattaa tagtgctatg atttttcatg ttcttgttaa ccaggtgttg ccatgtctca
     2461 tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt
     2521 ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc
     2581 tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc atctaacgga
     2641 acatatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg ttgttctgaa
     2701 ggtgctggat tcttatatca aatgaaagtg cctaatatgg ccgtagttga ggcaataaat
     2761 agagggacaa gtgacgttaa gctgaattag agtaaatggc gtgaggacac acgccattcg
     2821 aggttatagt ttgattaatt ttcgaaaaat tgcttcacag cccagccacc atgaccacct
     2881 ggagttatga atgaaacaaa acctttgtca tctacgctct ggatttctac cgtcattgat
     2941 tcatctaaca cgcgacattt attggatgta atgagactca gcatagcctg ttggtcattg
     3001 tcctggatgt aactgatcat ctcgtaaaag tcagttaact ttttacagtt aaatgtacct
     3061 tctttaagtg ttgcgctgtc tccgtgactt agtgcgaatg catttggcat cattgccaca
     3121 aagatgacgg taacgagtag ttttttcatt ttagcctctt ataattcaga acattcactg
     3181 atgttgtaag tgccttttct accatcatcg tttacgagat gtagcacgaa gatgatggga
     3241 ttggcatcaa tgaaagatac agagacgttc ccgctttcgg tgctatacat ctttgcaatc
     3301 tgtttttcac caatatatga gagatcccaa ctcactccgt tcaagctaaa atgcatctgt
     3361 ttgccgtcaa tatcgatgga ggattgtgcc gtaacggttt tcccattgct gccaatttga
     3421 ccctgctgaa gagcatcaca tttataggag cttgcataag tagatagagg tagtagcaga
     3481 agtgcagcaa aaattttact cttcatattt tccctttgct tggatttggg ttacctattt
     3541 tggggtagtc attataaaac tattaattag taaaacgaac aagttttcat ttgtttagca
     3601 atggtattgt ttaggtttga aattattacg tattttcata gcaataggtt ggataaacag
     3661 cagttcaggg aacaagttgc actatctaca tacttttccg ctttccattg catgttaggt
     3721 aatagatagc ctttgcccgt aagactaaaa atattagaaa tgactcattc gaataccttc
     3781 tattcacctt aatataggtt tgattattgt aatcgtttaa tgtgtcttat gattttttcc
     3841 tacttttcat ttgcataata tagggggcac agcatgctct tgaaattaat tattaa
//
LOCUS       DAZKFZ010000300         3895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000300 DAZKFZ010000000
VERSION     DAZKFZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            85..801
                     /gene="nanC"
                     /locus_tag="ACHOQW_004590"
     CDS             85..801
                     /gene="nanC"
                     /locus_tag="ACHOQW_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP9575511.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            821..1927
                     /gene="nanM"
                     /locus_tag="ACHOQW_004591"
     CDS             821..1927
                     /gene="nanM"
                     /locus_tag="ACHOQW_004591"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP9575512.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1992..2972
                     /gene="nanS"
                     /locus_tag="ACHOQW_004592"
     CDS             1992..2972
                     /gene="nanS"
                     /locus_tag="ACHOQW_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9575513.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            3095..3790
                     /locus_tag="ACHOQW_004593"
                     /pseudo
     CDS             3095..3790
                     /locus_tag="ACHOQW_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1086 a          893 c          937 g          979 t
ORIGIN      
        1 gcaaaaaaat attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct
       61 gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta
      121 ctgtgctttt cgtcaccatt aatttctcag gctgcgacac tggacgtacg tggtggatat
      181 cgtagtggaa gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga
      241 tggtgggcaa gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat
      301 gccgcactca atgatgttca ggttgaagtt aattacgcga ttaaacttga tgatcaatgg
      361 acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac
      421 gtaaaactgt cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac
      481 tggaaagctt accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt
      541 tgggatggat atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg
      601 ctatacagca aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat
      661 gcatttgttc tacaatacca tatgacgccc gatattacgc catacataga atatgactac
      721 ctggaccgtc agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt
      781 ggtgtgtcat ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc
      841 gcttgctatc ctaatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt
      901 accatttaaa agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag
      961 cgcaggtacg gcatggtaca agctggagac acaggccaaa gataaaaaat ggacagcgtt
     1021 agctgcattc cctggtggac caagagatca agcaacctcg gcatttattg atggcaatct
     1081 gtatgtgttt ggcggcattg gcaaaaacag cgagggcttg actcaggtat ttaatgacgt
     1141 acacaaatac aaccccaaaa ccaatagctg ggttaaattg atatcgcacg cgccgatggg
     1201 catggcgggc catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa
     1261 ccagaatatc ttcaatggct attttgaaga tctcaacgaa gctggaaaag attcaaccgc
     1321 tgtagataaa atcaatgcac actattttga caaaaaagca gaagattatt tctttaataa
     1381 gtttctgttg tcttttgatc cctctacaca gcaatggagt tacgctggcg aatctccctg
     1441 gtacgggacg gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg
     1501 cgaagccaaa cccggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa
     1561 tttaaaatgg aataggcttg ctcccgtctc atcaccagat ggcgtcgctg gcggttttgc
     1621 ggggataagc aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga
     1681 aaattaccaa aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga
     1741 tattcatctt tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc
     1801 ctacggagta tcattgccct ggaataatag tctattgatt attggcggtg aaactgcagg
     1861 cggcaaagcg gtgacggatt cagttttgat ctctgtgaag gataataaag tcacagtaca
     1921 aaactaacgc ttcagggccc cggtaaaggg gccctgctat caatttgtca tttcaattac
     1981 gcgaaattta tatgaacgca ataatatcgc ctgattatta ctatgttctt actgttgctg
     2041 gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc
     2101 ctcatcccag aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat
     2161 gtcactttaa cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcaga
     2221 gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac
     2281 tgcatattgc acggaaatta ctgcccttca ttcctgataa tgcagggatt ctcatcgttc
     2341 cgtgttgccg tggcggatcg gcttttaccg caggcagcga ggggacatat tcagaacggc
     2401 acggagccag ccatgatgct tgtcgttggg gaacggatac tccgctatac caggatttag
     2461 tcagcagaac gcgagccgca ctggtaaaaa atccgcagaa caaattcctc ggcgtatgct
     2521 ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacatcct caacacttta
     2581 atcatatggt tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata
     2641 ttactgacgc gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc
     2701 atgcgtatga agctatttat ggcaattatc aaaataatat tttagctaat attattttcg
     2761 tcgacttcca gcaacaaggt gcaagagggc tgacgaacgc gcctgatgaa gatccggacg
     2821 atttaagcac gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac
     2881 tgcgaagcag tcatttcagc tcagcagccc gtcgggggat tatttctgac aggtttgtag
     2941 aagcaatttt gcagttttgg cgcgaaaggt gagcgttcat taatttgatt tattcgcatc
     3001 catcagcgct tttaactcct ccggcgtgcg gcactataac gacttccaca acaaggagcc
     3061 attttcttat accactttca gtgaccatcc gcttatgcca ccaccctcta cgcaactgtt
     3121 tgcagagcac cttcccaccg agtggataca gcacttcctg acgctttctg ctcatgcgac
     3181 cgttcgtcgt cttaacctga gcgtggatgg cgaagcgggg atgaacctgc tggcgcccgc
     3241 agcgctgtca cccaggcgcg tcagcgcgtg ggggccgccc cggtggaatg gctcttccgc
     3301 cagaccacac agacatgggg cgcggaacgt tacctgaagg atgactggca cggcctgcaa
     3361 ctttttgcca ttgatggcgc acagttcagg acacctgatg aacccgagct gcgtgaatat
     3421 tatggctctg ccaacacatc cactgagcgg cagagcgcct accccgtcat gcgtctggtg
     3481 gcgttaatga atctgggaat cacattctac tgaatgccat gaccgcgcct accggcggag
     3541 cgatacggcg tgaggcaaca aaagcggcgt cagagatcag ctttaagttc gcgttccagt
     3601 ttatcgtgac agaaatgata gtgctgggaa acacggcgtc accggggact atcccgaaac
     3661 gactggagca tctgcggggg ctctggaagt ggtgttcata acaaaacgcc cccgaccatc
     3721 aagggcgagg gcggttaaga tatcaaaaac ccgttatcct gtgaaacaca gtgccgcccc
     3781 gcttaagtga acggcattgt gtttctgagg gaccttaatt ttccctgatt ctctgacgtt
     3841 ccttaccatt gatatttcct tcattcctca ccagagaaat aaaaaatcac tatac
//
LOCUS       DAZKFZ010000301         3742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000301 DAZKFZ010000000
VERSION     DAZKFZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(92..760)
                     /gene="artM"
                     /locus_tag="ACHOQW_004594"
     CDS             complement(92..760)
                     /gene="artM"
                     /locus_tag="ACHOQW_004594"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP9575514.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(760..1476)
                     /gene="artQ"
                     /locus_tag="ACHOQW_004595"
     CDS             complement(760..1476)
                     /gene="artQ"
                     /locus_tag="ACHOQW_004595"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP9575515.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1483..2214)
                     /gene="artI"
                     /locus_tag="ACHOQW_004596"
     CDS             complement(1483..2214)
                     /gene="artI"
                     /locus_tag="ACHOQW_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP9575516.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2232..2960)
                     /gene="artP"
                     /locus_tag="ACHOQW_004597"
     CDS             complement(2232..2960)
                     /gene="artP"
                     /locus_tag="ACHOQW_004597"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP9575517.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3178..3693)
                     /locus_tag="ACHOQW_004598"
     CDS             complement(3178..3693)
                     /locus_tag="ACHOQW_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9575518.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT          934 a          998 c          947 g          863 t
ORIGIN      
        1 gcatccggca caggcaccgt gctgatgtct gatgcgacgc tggcgcgtct tatcagacct
       61 acaaaacccc cggcgaatgt acgcagccac attaattcct tcgctcaaac gccagcgctt
      121 tgcgctcgat cagacgcatc atcagcgtca gcaggccgtt aacgaccaga taaataatcc
      181 ctgccgcgcc gaacaccatg acatcgtagg tgcgtccgta caacaactgg ctgtatccca
      241 tcacttccat cagcgtaatg gtgtaagcca gagaggtact tttgaacacc agcaccactt
      301 cgttggaata agaagagagc gagcgtttaa aggcatacgg cagcaggatc gccagcgtat
      361 cttttttgct cattcccagg gcgctacagg actgccactg accttccggg atcgcacgaa
      421 ttgcaccgta aaacagctgc gtggtatacg ccgcactgtt cagcgacaac gcaatcagcg
      481 cacataacca cggttctgac aacaaatgcc acagtgccgg atactcctgc aacgtcggga
      541 actgacccgg cccgtaataa atcaggaaga tctgcaccag cagcggtgta ccggtaaaca
      601 gcgtgatata accccgcacc agccacacca gcaccggcgt tttcagcgtc aggatgatgg
      661 taaaaatcaa cgccagaatc agtgccacaa tcagcgaggc aacggttagc gtcaggctgg
      721 tgtgcagccc tttcatcagt tcgggtaaat actcaaacat tagctgggcc tccgctcaaa
      781 acgtgtcgcg cgcaggtcaa tgcgtttgag aatgtactga ctgagcaggg tgatcaccag
      841 gtaaatcgcc gccgccacaa tgtaccaggt aaatggttcc tgggtacgag tagcgatgct
      901 tttggtttgc agcattaaat cattcacact aatcaaactg accagcgcgg tatctttcag
      961 cagcaccagc cactggttac cgaggccagg cagcgcatga cgccacatct gcggcatcac
     1021 cagacggaaa aagatagccg atttcgacag ccccagcgcc tggccggatt cccactgacc
     1081 catcggcacc gctttcaacg cgccgcgcag cgtttgcgag gcataggcgg catacagcag
     1141 tgacagagcg atgacaccac aaaggaacgg gctaacgtcg aagttttcaa tctgcatctg
     1201 caccgggatc tctacaatcc caagattgat agtgaagcca tccgaaagtg tcagcagcag
     1261 ctgcgaggag ccaaaataga taaacagcac caccagaatt tctggcaggc cacgcagaat
     1321 ggttaccagc gctgaacctg cccacgcgac aggacgccat ttcgcagact cccataccgc
     1381 aaagaacatc gccagcgcca gcccgacaat caatgcacaa acggcaaggc cgacggtcat
     1441 cccggcggcg cttgctaaag gaaaaaattc attcatcagg aattacttct ggaaccattt
     1501 gttgtagatg gtttcgtaag tgccatcttt cttcactttt tccagcgcag tgttgagttt
     1561 ctgctgcagc tcagtgttgc cctgacgtac cgcgatgccg aggccagtac cgaagtaatc
     1621 tttatcggtc actttatcgc ccactgccgc cagtttcggg ttatctttca gccattcagt
     1681 taccactgcg gtgtcaccaa atacgctgtc gatacgaccg ttttgcagat ccagttttgc
     1741 gttctggtag ctgtcatacg gcacggtggt gatttccggg tgcttatcca taatgaattt
     1801 ctggtgagtc gtaccattct gtacgccgac ttttttgcct ttcagctgat caacgctggt
     1861 gtatttaccc tgctgaccca caaacagggc agagttgtca tagtacggcg tggtaaacag
     1921 tacctgcttt tcacgttccg cggtgatatc catcccggcc atcaccgctt ccacgcgacg
     1981 gaatttcagg cttgggatca ggctgtcaaa tgcctggtta gagaaagtac aggtcgcatc
     2041 aatctcttta cacagcgcct gtgccaggtc gacgtcaaaa ccaacgatct ggttgtttgc
     2101 atcaatcgat tcaaacggag gataggaggc ttcggtagca aaacgaatgg tttcggcagc
     2161 tgtggcggaa agactaaaac ctgcaattaa cgcggcaatc agaacttttt tcattgttgt
     2221 tatcccgaat cttagtgaga gagatagttt ttaaatgctt cggtttgcgg ctcggtaaag
     2281 cagctcgcgt cgccttgttc tacgatatga ccgttttcca tatacaccac tcggctggcg
     2341 gttttacgcg ccacttcaac ttcgtgggtg acgatcacct gggtaatatt cgtttctgcc
     2401 agctcacgaa tgatgctgac gatttgtgcc gtaatttccg ggtccagtgc ggcggtcggt
     2461 tcatcaaaca gcagtacctg cggttccatc attaacgcac gggcaatagc aacacgctgc
     2521 tgctgaccac cagaaagatg cagcgggtaa cgatcgctat aaggtttgag acgcagacgt
     2581 tccagcagtt tttctgcacg ggccagcgcc tgatctttac tcaaccccag tacacggcag
     2641 ggcgcttcaa tcaggttttg ctgcacggtc agatgcggcc acaggttgta ttgctgaaac
     2701 accatgccaa cgttacgacg caaatcgcga atcgctttgt ctgagggtgt tttggtgaaa
     2761 tcgaaatggt tgcctgcaat gttgagcgta ccggagcgcg gcatctcaag cagattgagt
     2821 acacgcagca gcgagctttt acccgcgccg ctggggccaa gtaacaccag cgtttcgccc
     2881 tgtgggcaat ccagcgtgat atcgaacagc gcctgatgcg cgccgtagaa gcaattaatg
     2941 ccgtttaatt gaatactcat tgacactcgt atactggcag tctgatagct attgaggtcg
     3001 aagatagtac ctttgacaga ataattatgc aatatttctg ctttaaaagt taaaagcaaa
     3061 gcgcattatt caataaacat agcacaaaat aacgggggcg gtggtcggcg agcataaatg
     3121 tcggcattcc tcacgaaatg ccggacaatt tacggggttt attggttgat caaggcgtta
     3181 gcgattctcg atggactgac ggagcgtacc cgccgtggca tgaacgctac cgcctaagta
     3241 gcgcacatcg tcgatcaccc aacactggcc ttcctgaatc attaacactt catcctgcca
     3301 gccctggtca ccctgcttga gatccacgcg caatggaatg ttacgggcat cacgattagg
     3361 gatagtcgat gcactggcaa cgtgggcgct gtctggcaag gtggttcgac tggagaacgg
     3421 atcactgctt aacaattcac gatgactatt atcgcggctg gcatcgctaa gtagggttgc
     3481 cagtttgtca ctcaaatagg ggcgcagggc ggtaatgtca ttgctgcgat gttgaatgtg
     3541 gtagtcatag aattgttggg ccacgctgtc aggccccccc tggacacagg aaccggtgcg
     3601 gggaccatta tctttataag ctggcgtgac ggtggtacag gcactcagca gcagtgcaca
     3661 tggaataagt agagatagtg aacggtagcg cataatcatt tccttattag cttgctgtct
     3721 gcgtcacact acgaccaaac gc
//
LOCUS       DAZKFZ010000302         3611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000302 DAZKFZ010000000
VERSION     DAZKFZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..604)
                     /gene="ehxB"
                     /locus_tag="ACHOQW_004599"
                     /gene_synonym="hlyB"
     CDS             complement(<1..604)
                     /gene="ehxB"
                     /locus_tag="ACHOQW_004599"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP9575519.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIV"
     gene            complement(654..>3611)
                     /gene="ehxA"
                     /locus_tag="ACHOQW_004600"
     CDS             complement(654..>3611)
                     /gene="ehxA"
                     /locus_tag="ACHOQW_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP9575520.1"
                     /translation="TLTTKSAFNTASSSVRSAGKKLILLIPDNYEAQGVGINELVKAA
                     DELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDKLLQKYQKVGSKIG
                     GTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGEDISQNDIAKSSIEL
                     INQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNLPDLGPLGDGLDVV
                     SGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQYILAQRMAQGLSTT
                     AASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNYEGDALLAAFHKET
                     GAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTISGILEASKQAMFE
                     HVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFSRQHAVERAVAITQ
                     QHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQQVFDPQKGTIDLS
                     TGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWSVKGIKNHKGVYDY
                     SKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYDTVSYNKTDVGKLT
                     IDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFRTGGIPYD
                     VIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDDGDDYISG
                     GQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSGGTGDDKLYGGGGI
                     DLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLSDISFDDIAFKRVG
                     NDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVITDKDGREIKVDKI
                     PHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSGDERLASLYNLSLH
                     QNNTHSTTLTTTV"
BASE COUNT         1050 a          840 c          562 g         1159 t
ORIGIN      
        1 tcactataat aaatgctatt gtgataatat ttaacgttga aaacccccgg tgaactaaaa
       61 ccttatccat tacaacctga aaaaaaagag gtgttattaa cgcaagaaac tgaataaaag
      121 cagaaacagt taacacctca agtaaaatcc tcctgtattt cacaacagag gggataaacc
      181 aagaaaaatc aaatttagct aactctccga ctactgttgc tcttgacgta accagaatga
      241 cttttccctg atacagtttt tcaaactcat cccgactaaa agttagtgac tgatgttgtt
      301 ctggatcata aacaagatag cgtgatgaat ctttagtaat acgagacaat atataatgct
      361 taccgtcatc ccgccatatc aacgcaggta aagaaattat tgacaatctg gaaaaatgtt
      421 tttctacata ttttgctttt aagccaatag atttcgctgc cagtaaccat tcagtcaccc
      481 caaaatcttg tgtgtgggta ttatactgat gccttatagt ttcagcattg acagttatat
      541 tatgatattg tgcaagcaat atcagtgcat acagactatt atgagaacta catttactca
      601 tcatataacg atgaccattc ctcctggaat ggccatcacc tcctctttta gtctcagaca
      661 gttgtcgtta aagttgttga gtgtgtgttg ttttgatgta aggataaatt atataaagat
      721 gctaatcgtt catcacctga atcgaaccct gaaactgaag aaataatctt attaatatca
      781 ttggcaacac tggtgatatt aaccatgttt ttttcagatg ctatattact ggcttttata
      841 taacctgacc gttcattatt attatgaggt atcttatcaa cttttatctc tcgaccatct
      901 ttatctgtta taacctcaac aagatgatta tctgctcctg aggcatcttt cgcaaaccag
      961 tttttaaatg ttatcccatt gacatcattt gactcattaa atgaaagtac accattaatg
     1021 gctttattca tgataagatc atttccaact ctcttaaatg cgatatcatc aaagctaata
     1081 tcagataagt gcaaacgatc tcctttacct ccttcatctg caattgtatg atgaccatag
     1141 ttttgcccat aaacataaat atcattacca aaaccaccat tcagatagtc attaccttcc
     1201 cctccatcca gaagatcaat accaccacca ccataaagtt tatcatcacc agtaccacct
     1261 gacagaatat tataagctcc gtgtgcctga agctcatcat taccgcttcc tcctgtcaga
     1321 taattattac catcccctcc actcaattta tcgtttccac taccaccaaa taactggtcg
     1381 tctccctgtc ctccggatat ataatcatcc ccatcatcgc cgtatagtcg atcattacca
     1441 taattacctt cgatataatc gttcccatct gcgccatgga atatatcatt aaacttaccg
     1501 cctttgaatt catcatcatg ttttccgcca atgagctctt caacagaatg aagattatct
     1561 attacatcat aaggaattcc accggttctg aattcaaaat cacgatattg tattttctct
     1621 gttcgcttcc ctactgacac ctcctgttcc ttaacgactt cctgcaatac cttcacgtca
     1681 ccatacatat tttttgaaac tatatactca ccaggttttg atgctcctgt tgcatcaatt
     1741 gttagtttac caacatccgt cttattataa gatacggtat cataaccttc accagcaaat
     1801 acttctgatg agcctgctcc agaataaacc acatcgtctt tatctccgag ctcagaaatt
     1861 attctcgcct gatagtgttt gttattcttt tcaacaaact gaatcaattt tgaataatca
     1921 tatacacctt tatgattttt tatgcctttt acagaccatg tatccttacc atttacaata
     1981 agagatgtca tatattcata tttaccactc tgttttcttt ctctaacttc ttctcctggg
     2041 gtaaatgttg gtgttataaa tgtcaaaaca cttgatacat tacctgttga aaggtctatg
     2101 gtcccttttt gaggatcaaa cacttgttgt gtaaactcct tcggttgagc ctctaaaagc
     2161 cctccatttt ccagataatt aatatatgcc ttaccactct gactgcgatc agcattacgg
     2221 gttatgcctg caagttcacc gatcttctca tcccaatgtt gctgggttat tgcgactgct
     2281 ctttctactg catgctgacg agaaaaatca gcaagcaaag acagagagtc ttctaaaaac
     2341 gcagcatgtc ttgcgtcata tccattctca aaataatttt tgccatgctc cttttcccat
     2401 tcattgatcc gagcagcgaa tttctctgca acgtgctcaa acatagcctg ttttgatgct
     2461 tccagaatgc cagatatcgt accggttaat gcactcacca gcatgcttat cggggcccct
     2521 atgagggatg cactggaggc tgcactaact cccgcagata cagaactcag gacagtattt
     2581 attgttgtca gggctgcatc tatagctccg gtttctttat gaaaggctgc gagtaaagca
     2641 tccccttcat aattcaattt tttaaatcgt tcagaatatg attcaagctg cttagctcgc
     2701 tcaaatttat ctgcagcagc caggaaagaa agaggactga tagccagcat aacagccgat
     2761 gtgatcagac ccgcacttgc agctgttgtc gataacccct gtgccattct ctgagccaga
     2821 atatattgcg aaacagcttt accaacattt ccaagaacct gagttgtcag ttcgataccc
     2881 gctgcagctt ttgttcctgt atgtgcgtca ctgtttccca gaataaagct tgctgataca
     2941 gcagaaagaa ttccggagac aacatccagc ccatccccca gggggcccag gtctggtaaa
     3001 ttttgtaatt tcccaccaac agaacttaat cgaggtttac tggataaaaa tgagccaagc
     3061 tggttaagct gctcagaaaa tgaatcaacg gtactgttta tacttgatac tgtatctaca
     3121 agctgattaa taagttcaat actacttttg gcaatatcat tctgacttat atcctctcct
     3181 tcccgttgtt ttctcagcaa ttcatcaaga gccatgcctg ataaagcaat ccccgtaaaa
     3241 ttctgtagtg ctgagagaac tgttccggct tttcccagat tattacctac attttcagcg
     3301 gttcctccta ttttactccc aactttctga tacttctgca gaagtttgtc aagttgtggt
     3361 gcgaatattg caacaccacg ttcagttaat cctacaactt tttctgctgc accaaaaaac
     3421 tggtttgcaa tcgctgtatc atctcgttca gtacggtgta tttctattcc aagctcatca
     3481 gcagctttga ccaactcatt aatacccacg ccctgagctt cataattatc aggtattaat
     3541 aatatgagtt tttttccagc ggaacgtacg cttgatgatg ctgtattaaa tgctgatttt
     3601 gtagtcaatg t
//
LOCUS       DAZKFZ010000303         3608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000303 DAZKFZ010000000
VERSION     DAZKFZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..899)
                     /locus_tag="ACHOQW_004601"
     CDS             complement(<1..899)
                     /locus_tag="ACHOQW_004601"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFP9575521.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLE
                     ELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPV
                     DIGWSDVGSWQSLWDISLKSKTG"
     gene            complement(881..1390)
                     /locus_tag="ACHOQW_004602"
     CDS             complement(881..1390)
                     /locus_tag="ACHOQW_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9575522.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            complement(1393..2358)
                     /locus_tag="ACHOQW_004603"
     CDS             complement(1393..2358)
                     /locus_tag="ACHOQW_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFP9575523.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            complement(2361..3479)
                     /gene="gmd"
                     /locus_tag="ACHOQW_004604"
     CDS             complement(2361..3479)
                     /gene="gmd"
                     /locus_tag="ACHOQW_004604"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9575524.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            complement(3499..>3608)
                     /locus_tag="ACHOQW_004605"
     CDS             complement(3499..>3608)
                     /locus_tag="ACHOQW_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HFP9575525.1"
                     /translation="IFYEPTTLIELGLKRRNACADFLLDVVKQEIESRS"
BASE COUNT         1119 a          751 c          626 g         1112 t
ORIGIN      
        1 tctcctgttt tcgattttag actaatgtcc cataacgatt gccaagatcc aacgtcactc
       61 caaccaatat caacagggca tacaacacat ttttctgttt tttccattac agcaaaatca
      121 atagattcag caggacaatc ttgaaattgt tcttttgata atcgaataaa atctagatca
      181 atgtatgagg atgaggcaac ctgttcacaa acattgtaaa tgtcaggtct aaattttctc
      241 aattcctcaa gataaacaga tgccttaaac atgaatattc cactattcca atagtgatta
      301 ccagaagtca tatacaattc tgcggtttca cgattaggct tttcgacaaa cttatttaca
      361 taataaaaac cagtattttc atgcccttga agcggtacag atagttcacc tctctcaata
      421 tacccataac cagtttcagc atattctggt ataattccaa acgttacaat tttaccttga
      481 ttagcgatgg gagttgcatt tttaatagca tcacagaaaa cactttcttt agctataacg
      541 tggtctgccg caagaactag aagcaatgga tcttcctgag gattacgttt taacgcatga
      601 aacgcagata tcgctattgc tggtgcagta tttcgcccgc atggttctag aataatatta
      661 ccatttaatt tatttatttc ccttaactgt tcagccacaa caaagcgatg ctgttcattt
      721 gttatcacta atggtttttg acatgatagg cctgaaagtc gtagcaaagt cgtttgtaac
      781 aaggtgttat caccagagag ttgtaaaaac tgctttggat atagttcacg agaaagtggc
      841 caaagacgac tgcctgtacc accggccata actacagcaa ttattggcgc atcagacata
      901 ttatatcctt attatctaag ccaatcgcat catttgtttt attgttaaaa tatgcgcgac
      961 aattataatg cacatcgtca tcatttatca gctttgctcg cgatagccag caataagcat
     1021 tatgttgtga cttcggtaat ttcaaaatgt ttggttgtaa tttaagaagg aatgctataa
     1081 ctatataatg cgttgaaaag tctcccccca tactattgtc ttcgtagaag tgctgccaga
     1141 taccataaaa cttacccaca gagagaggca aacgaattcc tagttcaatt tctgtcaatc
     1201 gttcaaaggc tgtctgcaat ttttcatctt tcaacaccct accaccagga acgaaccaat
     1261 agccctgtgc cgggcggttg attcgttttc ctagcaaaat ttcgccaaac tcgttttcca
     1321 caatcaaatc tatagaaata agaggagtag accttacaat tgtggcaaag tcttgggaat
     1381 gtaaaaacat tattaccccc gatattgaag ttggttttca agaaaccagt tgtatgtatt
     1441 ttcaagaccc ttgtgaaggg taattttatg attccaacct agttgatgaa gaagcgttac
     1501 atcaagtagt tttcgagggg ctccatcggg ctttgttgta tcgaacgtaa tatgcccttt
     1561 ataacctaca acttttgcta ttgtttccgc aagctcacaa atcgtgcagt caatacctgt
     1621 tccaatattg atatgagaca acattacttt agtatttttt tgccatatat cgtatggcat
     1681 ctccatgaca taaatgcttg cagaagccat atcatctaca tgtaagaatt cacgctttgg
     1741 agtaccactt ccccaaacaa caacattcgg agaattgttt tccacagcat catgaaagcg
     1801 gcgcaaaagc gccggaatca catgagaatt acttggatga aaattgtcat ttggaccata
     1861 aagattggtt ggcattactg aacggtaatc acgcccaaac tgacggttat aagattcaca
     1921 taatttaata cctgcaattt ttgcgatagc ataaggttca tttgttggct caagtttccc
     1981 ttgtaataat tcgtcttcca taatcggttg gtgtgctaac ttaggataaa tacacgacga
     2041 accgaggaaa agcagtttat ttacattatt tttgtgggca gcatgaatga cattcgcctc
     2101 tatcattata ttctcatata taaaatcggc aggataagaa ctgttagcta aaatacctcc
     2161 gacttttgct gctgccaaat aaacctggtc gattttctgt gaagaaaaaa aatccaaaac
     2221 agcgctacta tccaacaagt tcaattcatc ccgagtacgt aaaaccaact caacatcatc
     2281 acgttgtttg aggcgtcggg taatagctga tccaaccatt ccttggtgac cagcaataaa
     2341 aatacgttgc ttattcatca tcattccaga gctaagctta cagaaaaacc atgcgatttt
     2401 aaaagagaat gttttttagc ggcttcaaga tctttggcaa ccatttcaga aatcatttca
     2461 gcaagagtaa tttctggtct ccaaccaagt ttgagattag ctttgctcgg atctccaagc
     2521 aaagtatcaa cttcagctgg tcggaaataa cgaggatcaa cagcaacaat gacatcacct
     2581 ggtttcacac ctggagcatc ctgtccttca accgaatcta caatgccttt ttcttcgatt
     2641 cctttaccaa caaagctcat cttaatacca agttgtgctg ctgccatttc gacaaactga
     2701 cggactgagt attggactcc tgttgcaatc acaaaatctt cgggttgctc ctgttgtaac
     2761 atcaaccatt gcattctaac ataatctttt gcatgtcccc aatctcgtaa cgaatccata
     2821 ttccctaaat acaaacatga ttccaagcct tgtgcaatat ttgcaagtcc tcgagtaatt
     2881 ttccttgtta caaacgtttc tccacggcgt ggagattcat gattgaacaa tataccatta
     2941 catgcataaa taccatatga ctctcgataa tttaccgtga tccaatatgc gtaaagtttt
     3001 gcaactgcat aaggggaacg aggataaaaa ggggtggatt ctttttgagg gatttcctga
     3061 acaagtccat ataattctga ggttgaagct tgatagaaac gcgttttgtt ttccaatcct
     3121 aaaaagcgaa ttgcttccag taaacgtaat gtaccaattg catcgacatc ggctgtatat
     3181 tctggagact caaaagaaac tgctacgtga ctcatagcag ctaaattata tacttcatct
     3241 ggctgtacct cctttagaat tctagtgagg ttagatgaat cagtcagatc tccatagtgc
     3301 aagtgaaaat ttgggttaga accatgtgga tcttgataaa tatggtctat gcgttctgta
     3361 ttaaaagatg aggctcggcg tttgatacca tgaacttcat accctttatc aagcaaaaac
     3421 tcagctagat aagatccatc ttgtccagtt acacctgtaa taagagcgac tttagtcatt
     3481 atatacctct tgaatatatt aagatcggga ttcaatttct tgtttcacaa catctaaaag
     3541 aaaatctgca cacgcattgc gtcttttgag acccaattct atcagagttg taggttcata
     3601 aaaaattt
//
LOCUS       DAZKFZ010000304         3572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000304 DAZKFZ010000000
VERSION     DAZKFZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..891)
                     /locus_tag="ACHOQW_004606"
     CDS             complement(<1..891)
                     /locus_tag="ACHOQW_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFP9575526.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDASF"
     gene            complement(901..1581)
                     /locus_tag="ACHOQW_004607"
     CDS             complement(901..1581)
                     /locus_tag="ACHOQW_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP9575527.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1568..2035)
                     /locus_tag="ACHOQW_004608"
     CDS             complement(1568..2035)
                     /locus_tag="ACHOQW_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFP9575528.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2035..2571)
                     /locus_tag="ACHOQW_004609"
                     /pseudo
     CDS             complement(2035..2571)
                     /locus_tag="ACHOQW_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2635..3282
                     /locus_tag="ACHOQW_004610"
     CDS             2635..3282
                     /locus_tag="ACHOQW_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9575529.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT          992 a          852 c          830 g          898 t
ORIGIN      
        1 aaaagaagca tcacctaaaa gtatcataac tttatttgat attatttttg aaataatacc
       61 accattaaac gaaaacacta atattgaaaa ggccattgat ggtataaaaa gcgcatcata
      121 ctgtaatcca ttgctatttt gactttttgc caccatatag acagacatta ggataaagac
      181 tacagatgat atttcagcaa atgatagaac taaatatgac ggctttgact taaactcaaa
      241 atctccggcc tgattgctgc ggattgtctc tgccagcctg cctcggtcaa tctcacgacc
      301 agccatcttg tcctgaacat tgaagtagtc aatcggaccg agagcagcca tcccaaggtg
      361 ataaacaaac tcaccaaacc ctgaaggatt ctgctgatac atctgagcaa cgttgttagg
      421 gtcaacaccg acgcgcgcca gttccttggc gttgttttgc agccatgatt gcattgcttc
      481 tggagacgag gccgcaaggc gtgcgccagc cgctaaggtg ccgatagaat tgcgctggtc
      541 ttcgtctgcc cacttcatac cagactgaat cttctctaat tgaccaggat atttggtcat
      601 cagatctcga acctgctgtc gatcgccgga ctggatggct gccgcatatt ctttttggaa
      661 tgcagcatcc gcttcctgtt gctttgcggc ttgagatgtt tgagcgacac taccaagtcc
      721 ctgcaacgcc tgaaggccga tgttattgcg acctgaacgc tccatttcgt tgttctggcg
      781 aatataggcc aacgcctcac ttacatcact tgcctttggc gcatttgagt tttgcccacc
      841 gataccagca agaaaaccgc ctgagttgat tccttgttgc caagtagcca tattcccacc
      901 ttaaaacaat gatccaagac caccgataac accgccagca agagcaccaa taccagtccc
      961 aataacagga actgcactac caatcattgc acctgatgcg gccccaccaa tggcacctgt
     1021 agcaaaactc tgtaggcctg aaggcttatt cgcatttgct gccgatgctg ccgcctgctg
     1081 ttgatacaat tggctgacat tgttagcgta gttctgtccg gcgtttgcct gacctgtaag
     1141 agcaccaagg ccgatatttg ccagattgtt gtagttgttc atctgacctg acagccagtt
     1201 ttgaccgagt gtaggtgcga ttgctgctaa ctggtttcct gttgctgtag agcctaatcc
     1261 acccgttgcc tctgctgctg ccagactctg atagcgcgcc tgccctgcaa ggtctttata
     1321 ctgctgagag ttgtaatact ggttaagcgc ctgaccttgc ccctgaagag aggaaagatt
     1381 ctgcaactgt gatacgtact gctgagcgag tggcgtgaac ggtgcaaggt tttgcatgtt
     1441 cgtctgccac atttcacgct gcagttcgat gcccttttca gttgcgcgtg cctgggcttt
     1501 agatccggag tcgctgccgc ctttcatata cccattcatg ggaagcaatt tattcttgaa
     1561 gctttcgcta agtactaaca tttaatagct cctcatattt cgaacgaggt aattgataga
     1621 gggtgattcc taccggtttt ccgttactca tgtacgcatc atcaaggtga ccaacacggg
     1681 tagcgccaag caaacggata attgcccgtc cgtatttggt ggtgtcagga accatggtga
     1741 tgctgttaag gaatggtgag ttttcgagaa gccatttgca gaataatcga tgcccttgta
     1801 gtgcatattc gccacggaat ccggggtcat acaccgcatg gcattcaaca acgctatgcc
     1861 agaagttacg cacttcatga actccagcca gcactaatcc ttcgtagatg ccgagatata
     1921 ccgcatcagg cttgatgtag tatttatctc cactgtctac gatattcccc gtgtttaccg
     1981 ggttgttaag gaattctgca agtttcaccg ggttatcgat gagctttatt tccatcactg
     2041 ctccgcaatg attttgatgg ttgtggcagt aaacgccgcg ccattcgact gaatggttaa
     2101 cgtgctgcca tttgtggcaa gaaagccgtc tttatccacg ctgaagaacg tagctaacag
     2161 gatgttgtcg gttgttgttg ccgagttgcg actgcttact agcgtgtcag gaacagaacc
     2221 ggagaatgtt agctgcattg acctgttggc ggttccgctg ggccacgtcc cgacaatcga
     2281 cagcttgaag aacaaggttt tgttctcgtt gaacacaacc atcttgttgt taacggtgtc
     2341 gaagaatggt gccaacgtcc cggatgacgg cgtaagcgtt ttcagcaggc taacaaggtt
     2401 ggtcggcgct gtcgggatgg ttactgatac gccagagtaa acaacctctg acttcttgcg
     2461 cgtagtggca tactccagag cattaatgcg cgtttcatgg tctgaaacct gcgattccag
     2521 cgactgaact ctggtatcaa gcgatgcaat atcgctttca ttcaaggcaa tcagtgggtt
     2581 tacgtcatgg agtgggctca acaccagtca gagccgcatg tacacgcaat aatcatggtg
     2641 aacgaaagaa gcattaatgg gcaagctggc aaaattgtca gcaaatgggt aacactggca
     2701 aacaatgcaa tcccatcaaa tgttcctccc cagtgggatc tagatttgaa aggtcagcaa
     2761 tattcagagc ctacaaagtg tataggtaaa cagattgatt acatggcaaa gcccgaaact
     2821 aagttaaatg actttaatag agaggcgagc aagagcctat atgattggtc agatgccaac
     2881 gtttggggtt attcgaatgg ctgggcgcgg catgagataa aaaaagaaac actttctatc
     2941 actggctttc atgctgtaag acgcatacta aaagccgttc aggcatcaag gaaagacaac
     3001 tacaaagcta agcgacagat acagcgtaca ttgaaacgac aagacataaa tcgatcacca
     3061 atcaccccgt tccaagcacc gttaattagc aaacacgatt gggatttatc agtgatagcg
     3121 gtcaccaaag aggcagagac agagcgcagg gaattactat cctcacagat aataaactcg
     3181 aacaaagtac aaacccaccc actgttaagg atgttaagaa gagcacgagt gagagcatcc
     3241 agagccaaaa gagaaagcgg aggttatgac cattactact agtagtgaca gatcgtgaca
     3301 aggcggtgca aaaaacgacc aataataaga gagatatgcc tgccaaaccg ccaaaagcgc
     3361 ggagcgcggc aaacgccgcg agaggcgcaa gaaggcgaac ctatccattg aaacggtcag
     3421 aagcgcatcg cgctcgacga ccgaagtccc cactaacacc gttaacgaaa gatcaaagtc
     3481 ttagaaaaaa atgacgtttc tttgatggac ttccatccgt aaaaatcaaa ttttgagaaa
     3541 aagtacttat agaaattaat cgctctataa gg
//
LOCUS       DAZKFZ010000305         3568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000305 DAZKFZ010000000
VERSION     DAZKFZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(17..1174)
                     /gene="intS"
                     /locus_tag="ACHOQW_004611"
     CDS             complement(17..1174)
                     /gene="intS"
                     /locus_tag="ACHOQW_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFP9575530.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1336..1410)
                     /locus_tag="ACHOQW_004612"
     tRNA            complement(1336..1410)
                     /locus_tag="ACHOQW_004612"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1375..1377),aa:Arg,seq:cct)
     gene            complement(1486..2418)
                     /gene="yfdC"
                     /locus_tag="ACHOQW_004613"
     CDS             complement(1486..2418)
                     /gene="yfdC"
                     /locus_tag="ACHOQW_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9575531.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2712..3467
                     /gene="mlaA"
                     /locus_tag="ACHOQW_004614"
     CDS             2712..3467
                     /gene="mlaA"
                     /locus_tag="ACHOQW_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP9575532.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          850 a          886 c          847 g          985 t
ORIGIN      
        1 agaatcgttg taataactac tccaccttcc catcaagcca gtccgcccac cactgcatca
       61 tttctctgcg cttatcgaga tactgagcat ggttgtaaat cccacgcaca gatccgccgt
      121 tggcatgtgc tagttgcact tcaatagcgt cagcaggcca ttcgtgctcg ttcataatcg
      181 tgctgaattc atgcctgaat ccgtgaccgc tttccagacc ttcatagccg atttgtttga
      241 tcacaagcag tacagcgttc tcacagattg acttcttctt atcgttgcgc ccggcaaaaa
      301 caaactctga tactggttta gtgatggagc ttagcgtagt gagaagttca acaacctggt
      361 ccgacatcgg aaccacatga atcttgcgtc ccttcatcac actggcctcg atggtgataa
      421 tcctgttttc aaaatcgacg ttcttccatt gcatggaacg aagctctttc gttcttagtg
      481 ctgtgtagcg taaaactttg gtcgcaatga gagatacgat acttcctgaa aatgttgcaa
      541 gtgctttgtt gaatgccggg atctggtcgg caggtaaaaa cgggaagttc ttcttgcggt
      601 atcccttcat ggcttcagca aggtcaggtg ccgggttata tttagccctt ccggtgacaa
      661 tagcgtaacg gaaaacctcg ccgcatcttc tgcgtgcttt gttggctcgc tccattgccc
      721 cgcgatcttc aaatctgcgg attacttcca gcagttgcat cggctcaata tcctgaattt
      781 caaggccgcc gatgataggt aaaatgtcgt catcaaacat ttttgcaagt tcagtcgcat
      841 aggcggctga ccagacttgc ctcttatgct cgtaccattc cttgtaaatc gcactaaagg
      901 aattgttgtt agacgaagcc tttttcgcct ttaccggatc gatgccaacc gagatgtctt
      961 tcctcgcagt ccatgcttta tcccttgcct cttgcaaagt cataagcgga tattttccga
     1021 cggtcaggat tttctcctta ccgtcaatct tgtagcgaag ctgccatacc tttttccctg
     1081 atacagggac ataaaggtac aggccattac catcgagtag gcggtatggt ttttctttcg
     1141 gctttgctgc ttcaatctgc ttaacggtga gcatgggtaa aaatccggtg ggtaaaatta
     1201 ttttatccac tttttacccg tcatggcgtg cggctgtcaa cgatctgacg cgaaccatga
     1261 cgaactgtga atctacggaa ggcttgatat tcaggggatt ttgcggactg gtacggatgg
     1321 aggcgaactg ataaatggtg tcccctgcag gaatcgaacc tgcaattagc ccttaggagg
     1381 ggctcgttat atccatttaa ctaagaggac aatgcggcat gagtataccc gctaatggac
     1441 tgcggggtaa gtacgctgcc gctcgattgc ttaaaccctc gccatttatg ccgggttttt
     1501 atcatttttc ttaatgtttt ccgcacgttc tgctttttgg cgtgcttctg ctttgcgctt
     1561 gttgctcatg tcgttacgga tctgtgcatg actcattaac gcgaagataa aggtgccgcc
     1621 gcagatgttc cccgctaaag taggtagtgc gaagggccag atgaaatcgc tccagtgcag
     1681 cgtaccgtta aacaccagat agaggatttc aacagaaccg acaacgatgt gagtggtgtc
     1741 acccagggca ataagccagg tcatcaatat aatcaccaca atctttgccg cacccgctgc
     1801 aggaaacatc cataccatag tggcgatcag ccagccggaa atgatcgcgt tggcaaacat
     1861 ctcgctgggg gtgttcttca tcacatccat gccgattttg acaaatgcat cgcgagtttc
     1921 ttcattgaag attggcatat attcaaatgc ccacgccgca atacctgtcc cgagaatatt
     1981 acccagcagc acgacgcccc ataaccgcat aagtaagcca acgttgctca ttgtcggttt
     2041 ttgcatgacg ggtagtaccg cagtcacggt gttttcggta aataattgct ggcgggccat
     2101 aatgacgata ataaaaccaa aggtataacc gagattctcc agcaagaagc tgcccggcac
     2161 accttccagt tcgacatgaa atatcccttt tgccagtaat gaagcgccca tcgacagacc
     2221 cgccgcaatg gctgaccaca gtagtgccat tgcgtcgcgt tccagctctt tttcaccatc
     2281 ctggcggata tgctcatgaa ttgccatcgc ccgggagggg agtcgatctt catctatttc
     2341 tatttttttg ccacgctctt tttcttcgct ctcaacttca atttcgtcgc tgtgttgatc
     2401 aattttgtcg ttgtccatgg tctcttctct tgaattagca cgtatagcta aagcgtagcg
     2461 gcttttttgc tcgcaactgg cgggagttac tttgaaaatg tagaaaaggc cgcgtttgcc
     2521 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
     2581 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
     2641 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
     2701 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
     2761 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc
     2821 gcaccatgta caactttaac ttcaatgtat tagacccgta tattgttcga ccggtcgctg
     2881 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaacgg tttgagcaat tttaccggca
     2941 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
     3001 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
     3061 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
     3121 ttggtcatta tggcgtgggt tatggcccgt acgttcaatt accgttctac ggtagcttca
     3181 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
     3241 cctggccgat gtctgtgggt aaatggacgc ttgaaggtat cgaaacccgc gcacagctgc
     3301 tggattccga cggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
     3361 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
     3421 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa
     3481 aaggtgagtc gcaaaactca ccttttttgt tgttatccct caattaccac aagtaaaagg
     3541 agataaaccg acaatgaccc ggacgtaa
//
LOCUS       DAZKFZ010000306         3567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000306 DAZKFZ010000000
VERSION     DAZKFZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.308"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            26..1387
                     /gene="espK"
                     /locus_tag="ACHOQW_004615"
     CDS             26..1387
                     /gene="espK"
                     /locus_tag="ACHOQW_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFP9575533.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            1448..1723
                     /locus_tag="ACHOQW_004616"
     CDS             1448..1723
                     /locus_tag="ACHOQW_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP9575534.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(1803..1916)
                     /locus_tag="ACHOQW_004617"
                     /pseudo
     CDS             complement(1803..1916)
                     /locus_tag="ACHOQW_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
     gene            complement(1951..2169)
                     /locus_tag="ACHOQW_004618"
                     /pseudo
     CDS             complement(1951..2169)
                     /locus_tag="ACHOQW_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2261..3489
                     /locus_tag="ACHOQW_004619"
                     /pseudo
     CDS             2261..3489
                     /locus_tag="ACHOQW_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT         1137 a          811 c          795 g          824 t
ORIGIN      
        1 ttatttctaa aaatggaatt tatcaatgct tcctacatcg caattacgac cgaccgggac
       61 attctgctcc tattccgctg aaacatcagc agacatcaaa agcgaaatca caccaattca
      121 gatagaagaa gcgcgggcca gtggtcgttt atatatcaaa gattgtgata ttgagtatct
      181 gccacagtta ccaaacgaaa taacatcagt tacaatcgaa aactgcaaca acctgacaac
      241 ccttacagga ttgccggtta atacacaaaa cctctccgtc attaactgtg aaaaattaca
      301 aatcacagac atgccatcaa ccgtaaaaaa tctacatatt gaattaactg attcaccatt
      361 tatacatttc atatctgaag gcatcgagtg cctgacggtt tgccactgct atatatctgg
      421 agtgccagag agtgtccgct accttgaaat aaaaggtagc gccacagaca gcataaaaaa
      481 tgttccaaac gggttatcat ctctcagcat caatagctat aacccggaga atcaggccag
      541 aattgacaac ctgatatcac cgtcactgaa gacgctatcg ctgactggat gtagcaatat
      601 tatactgccg gagaaacttc cggagagtgt gacatcggta accattcatg cggagcagaa
      661 aaccacgtgg aacatcggtg ttgaagggat gcctgatggg ctggatcttg atttacaaaa
      721 tgtactactc tctccagatg tagttaaagc aaaaaacatc acctttcagg gcaacgctct
      781 ggatgtggcc ttacactttc gcgagggaga cattgtctat ggactatctt cacccagaga
      841 aaaacttgtc aacagcatta aactagttaa cgacttttcc aaaaaagata ttataactca
      901 gaatacgtta acaaacgcag tatgggaccc cagaacacct cgcaaatata agcaagatcc
      961 acttatcaaa agagcattaa atgaacacga aagaggaata aaatttaaac aacacttaaa
     1021 gaatcacaat aattataatg ttaccatggc cgacctttcc gtatacaatc gcgacaaatt
     1081 atgggcaaaa acaagcaagg ccggcctaga gtttcagaca ttaacacgca ataaaacggt
     1141 tattttttgt gcggatgagc ttgtcaactc actcaaactc atagctaaca agtcagaggg
     1201 ctatggccag agtattaccg ccagcgaatt acgatggatt taccgtaata aagacaacaa
     1261 ccaaataatg aaaaacataa aattttatct acatggcaaa gagataccag cagaaagaat
     1321 attagataca ccagaatgga aagactatcg tccaaaatac tctggttcca catataaata
     1381 ttcttaatga taccaactta taacggaata gcataaaaac acttttcatg gagcaaagga
     1441 gaaaacaatg ccattttcaa tcaaaaacag attttcaagt tcacaagtac attacccgga
     1501 aatatccggt cccataaaag acaagccagc gtcaaagaac tgcatactta catcaacaac
     1561 atgtaatgta gatagctata cagtgtacca aaaaaaagcc tgtagttttg acatgcgccc
     1621 acccggcgca ggagaaagaa ccccaaaact aaaactctca gttactgaga tgacatggct
     1681 atctaaaact atagaaacag agatacacaa cacaaaagaa tagcaaccac ttaccagaga
     1741 acacaaaaag ccacagaccc gaaacacctg actgcaagca accacctcca caggaggtgg
     1801 ttttaccatg aacttcctgt gtactgactt gtgttcataa taatattttt gtgtttaaac
     1861 tcaataaagt cacaaaaatg attgtaatca tgcaatgtag taaaattaaa atatttgacc
     1921 ctgtacacga ttctgtgtaa atgccttttc tcagaagtga ccgtccaggc ggtcaccgaa
     1981 ctcgataata aagcggctca ttgccatacg ccagtcccgc agtggcatcg tccatttctg
     2041 tgaagccgcc tggattgcca gccacacgac ctttttcact gagtcatccg tcgggaacac
     2101 cttgcgtttt ttgatggcat gccggattac gctgttcagc gactcgatgg cgttggttgt
     2161 gtagatgact agactggccc cctgaatctc cagacaacca gtatcactta aataagtgat
     2221 agtcttaata ctagttttta gactagtcat tggagaacag atgattgatg tcttagggcc
     2281 ggagaaacgc agacggcgta ccacacagga aaagatcgca attgttcagc agagctttga
     2341 accggggatg acggtctccc tcgttgcccg gcaacatggt gtagcagcca gccagttatt
     2401 tctctggcgt aagcaatact aggaaggaag tcttactgct gtcgccgccg gagaacaggt
     2461 tgttcctgcc tctgaacttg ctgccgccat gaagcagatt aaagaactcc agcgcctgct
     2521 cggcaagaaa acgatggaaa atgaactcct caaagaagcc gttgaatatg gacgggcaaa
     2581 aaagtggata gcgcacgcgc ccttattgcc cggggatggg gagtaagctt agtcagccgt
     2641 tgtctccggg tgtcgcgtgc gcagttgtac gtcattctca gacgaaccga tgactggatg
     2701 gatggccgcc gcagtcgtca cactgatgat acggatgtgc ttctccgtat acaccatgtt
     2761 atcggagagc tgcccacgta tggttatcgt cgggtatggg cgctgcttcg cagacaggca
     2821 gaacttgatg gtatgcctgc gatcaatgcc aaacgtgttt accggatcat gcgccagaat
     2881 gcgctgttgc ttgagcgaaa acctactgta ccgccatcga aacgggcaca tacaggcaga
     2941 gtggccgtga aagaaagcaa tcagcgatgg tgctctgacg ggttcgagtt ctgctgtgat
     3001 aacggagaga gactgcgtgt cacgttcgcg ctggactgct gtgatcgtga ggcactgcac
     3061 tgggcggtga ctaccggcgg cttcaacagt gaaacagtac aggacgtcat gctgggagcg
     3121 gtggaacgcc gcttcggcaa cgatcttccg tcgtctccag tggagtggct gacggataat
     3181 ggttcatgct accgggctaa tgaaacacgc cagttcgccc ggatgttggg acttgaaccg
     3241 aagaacacgg cggtgcggag tccggagagt aacggaatag cagagaactt cgtgaaaacg
     3301 ataaagcgtg actacatcag tatcatgccc aaaccagacg ggttaacggc agcaaagaac
     3361 cttgcagagg cgttcgagca ttataacgaa tggcatccgc atagtgcgct gggttatcgc
     3421 tcgccacggg aatatctgcg gcagcgggct tgtaatgggt taagtgataa cagatgtctg
     3481 gaaatatagg ggcaaatcca gatggattta tcgtaataaa gacaacagcc aaataatgaa
     3541 aaacataaaa ttttatctgc atggcaa
//
LOCUS       DAZKFZ010000307         3456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000307 DAZKFZ010000000
VERSION     DAZKFZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.309"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..158
                     /locus_tag="ACHOQW_004620"
     CDS             <1..158
                     /locus_tag="ACHOQW_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9575535.1"
                     /translation="KSGLSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFL
                     IEVKNER"
     gene            148..477
                     /locus_tag="ACHOQW_004621"
     CDS             148..477
                     /locus_tag="ACHOQW_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575536.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(547..1875)
                     /gene="dgcI"
                     /locus_tag="ACHOQW_004622"
     CDS             complement(547..1875)
                     /gene="dgcI"
                     /locus_tag="ACHOQW_004622"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9575537.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1883..>3456)
                     /locus_tag="ACHOQW_004623"
     CDS             complement(1883..>3456)
                     /locus_tag="ACHOQW_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFP9575538.1"
                     /translation="SAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYL
                     MTTRVFRRQFSEMTELINTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEI
                     ERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKI
                     VRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRI
                     HKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIR
                     HAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINEL
                     GYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANS
                     LCVEITETVIERINEHFYLNIEQLRKQGLRISIDDFGTGLSNLKRFYEINPDSIKVDS
                     QFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKP
                     MPFSAWDKSGKLVKE"
BASE COUNT         1031 a          804 c          691 g          930 t
ORIGIN      
        1 ccaagagtgg tctaagcagt aaaggaagca aagaaatcga agatgatgaa ctggcagcct
       61 acaaaaaaat ggcaaatgca tttctggcct ttagcaataa gcaaatagag gatttgatcg
      121 aaactggctt cttgattgag gtgaaaaatg agcgataaat tacaaatgat gctggaagat
      181 gcaaaggaac tgaatgcact gggcctgatg tctgatagtg aggttgatgc cattgccgtc
      241 atggtcaaag cccgtgaagt cagcgcccgt atagccaaag tcaaatcaat gacgggtgaa
      301 gagatcaaag ccatccgcac ccgatacggc atgtcacaat ccgtgctggc acatacaatg
      361 ggaatgtcaa aagaaagtgt ctcgaaatgg gaacgtaatg aaataaaacc gagcggcccg
      421 gcactacgta ttttgaatac attggcaata aaagggccgg aagtttttgc aaagtaaaat
      481 gtttgcaaat gatttaccac attcattagt accccttgat tcaggggtac taacactatt
      541 ctaattctac tgccagctaa tctgagccct gccgtttttc ttactcttat acagccgctc
      601 atccgccagc attttcaagc gttcgacggt agtacattca tttgaatgcg ctacaccgat
      661 actggtgctt aaattaatgc ttcccacctg gaaatgaacg atttctttta gaatcgatag
      721 taactcttcc agcgtgccgc cagtataaat caccgcaaat tcgtcaccgc cgaagcggta
      781 gactttacct ttatctaccg ggatctgtga tttaaggatt ttcaccactt tcatcaacac
      841 ttcatcgccc agcaaatgac cgaaggtgtc gttaatattt ttgaatttat cgacgtcaaa
      901 caggcacaaa taaccgcctt tttccggcag cgcttttaaa tcttcgtcaa aggattttct
      961 acccattgct ttggtcaggt cgtccaggaa cgccgagtta tacatactgt agagtttttt
     1021 gacatcggca gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat ggttattaat
     1081 aatggcttct ttctccacaa taccacgatt aatcactctg cgtaccagat agaggcaact
     1141 ggcacccgat atcactgcca ccaatgcggc aaagaaaata atcaatgtac agacccgcaa
     1201 ataaacatct tttttatacg caaatacgta caatccggtc ggcaaatagg cgaagtgctc
     1261 catcgcttcc gaaacggtga agatggcgtt tttagcatta tattcatcac cggtcaccgg
     1321 tttaccgttc ttaaacagag agaacatcag gttctcgctg tccatcgcca gcgattttga
     1381 cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga ctacgattgt gagttaccgg
     1441 atagaggtaa tagacgaagg ttttaccgtc aaaagagata aaccctgtat ttttgacatg
     1501 tcgatacttc acaacctgca taatatctgc acaataattt ttctcgaact cctttacctg
     1561 aatacattcg tcactggaaa tcagataagg accgacatcg tacaaataag agggtgaaac
     1621 cgagtcggcg taagctgtgc gtaagccaac caacagctgt tcctccgtgg gatttagcgt
     1681 gttgataaga tcaattgcca cgctacgccg atattcagca atatttgcca ccgcaaaagc
     1741 agtatcaata acggactgtt tcaggctata cacccgttcc tttaccattt gagtgtagat
     1801 cccgcaggca actgctgaaa tcatgataaa aatcagcaga ctgactgtaa gtacgaactt
     1861 attgattctg gacatacgta aactactctt ttactaattt tccactttta tcccaggcgg
     1921 agaatggcat gggtttctga tacagatacc cctgagcctg aacacatcct aatttgatta
     1981 attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat caccgggatc cggttatagc
     2041 gtgccaggtc gaaaataatg cgcacaattt ttcccgcagt accgaaaata tcgccggtaa
     2101 attgcgagtc cacctttatg ctgtctggat taatttcata aaaacgtttc aggtttgaca
     2161 aaccggtgcc aaagtcatca atcgatatcc gtaacccttg tttacgcagt tgttcaatat
     2221 tgagataaaa atgttcatta attcgctcga tcacggtttc agtgatctca acacagaggc
     2281 tgttcgcctt caggccattt tctttcatga tggtggtaaa acgctcatga aagtgcggtt
     2341 cgtttaactg ccagggcgaa acattaatat gcagtaagaa atcatccttc agcgatgcac
     2401 gctgactaaa atgtctgaat tcacccattg ccgttttaat aatctgataa cccagctcat
     2461 tgataaaccc gatatcttca gcaatggtaa tgaacttcag cggtgaaatg atcccccgct
     2521 ccgcagacac ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg ttaatatcaa
     2581 cgatcggttg atagacgagg aatagttcgc caatttccat tgcatggcga atattacgcg
     2641 caatccagat attctcttct atttcgttga gtttcgcgtc atcacagaaa ataaactcgg
     2701 agaacatatg gtcacggata ctggaaatcg ccagtttcag tttttctgcg taactccagg
     2761 cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga ttgcggtagc
     2821 caaagctgct gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc tgtacatttt
     2881 cccgacacag aagtaaatag agatcggcat tgaatgtcac gatatcgcag cattgtgcat
     2941 atttctcagc aattcgctga cacaatttcc tgacgatttt attgcgttca tcaacaccaa
     3001 acacagcttc cacggcttcc agaccacaca gtttgatgat ccccacagcc agatactgat
     3061 tattgttgga ctcaataatc gccatatttt taataaaacc actttcctga tcgtaagaga
     3121 tcagtgagag caatttattt gaccgttcaa tttcggcatc tttcatttcc gcgatcgaat
     3181 ttttgatgct gataatctct ttcgcatcgc cttcgcgaat tttcagagcc tcgatctgat
     3241 ccgttgagtc aggcaaaaac gccagcgtat taatcagctc tgtcatttca gaaaattgcc
     3301 gacggaacac acgagtggtc attaaataaa ccaccagcaa tacgataata atgaacgcaa
     3361 acggcagata aaaaagttta tctgatgcgt tcaataaatt accgcccgga taataaaaat
     3421 agatttcgaa taatgcgtta tttggcaccg ctgatg
//
LOCUS       DAZKFZ010000308         3451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000308 DAZKFZ010000000
VERSION     DAZKFZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            240..749
                     /locus_tag="ACHOQW_004624"
     CDS             240..749
                     /locus_tag="ACHOQW_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575539.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            838..1476
                     /locus_tag="ACHOQW_004625"
     CDS             838..1476
                     /locus_tag="ACHOQW_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9575540.1"
                     /translation="MRTRIDYLADKYSFTELNERPHLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDIGQKNVLFNDKRFGCVYSLKS
                     SLSGVPDTYRYHLSHRIRRVVANENTSLPYQQIAREVKAPRERLKYALETGLQVTALD
                     GLFWFGSQRIAADIQRLRKSGMRITTSAISVYDSITVTRRLCPVYRIVANNK"
     gene            1813..3432
                     /locus_tag="ACHOQW_004626"
     CDS             1813..3432
                     /locus_tag="ACHOQW_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9575541.1"
                     /translation="MARIPTKKAKAKKGFEETLWDAANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFETRRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
BASE COUNT          925 a          777 c          892 g          857 t
ORIGIN      
        1 tatatgtctt accgggcatc tgttcttaca ttaattaata tattgctatt taagtattgg
       61 tggtattgtc agtaacattc ttttggttaa tttagttatt attcctcact gattaattgt
      121 ccttccgttc ttattgtttg taacttgtgt ttgtatcgta cctttcaggt gttgatgcgc
      181 gctttactgt gtctgcaccg gcttgacaaa attcaacagg gtttgaaaag gaacatttcg
      241 tgcaaataac cgaagcctta atttcagagc cgggggaaat ccggcgtttt gttcagcagg
      301 cggtggacca ctggccaaat ctgttggcat ttcacttcac gctctattcg gcagaaggaa
      361 tctatggtca acagattcag acattttgta gttcctttca ccgacgggta cacgaacgta
      421 ttacggagca taatcacacg gtcagcccat cagcgccggt ggtattacgc tggttgcggg
      481 aacaacatga aggagcacag atacgatgtc tgttgctgct cagccagacg agtatttgcc
      541 atccgcgagt cggcgtcatg gctgatgaag aatgtgcgca actggtggat ttactgcaac
      601 agacctggtc ggtgataagt gctggcggac aatgccgggt ggaaagatgt tttcgggttg
      661 cccggcctgg ttcatcaggg caatacgtgg cgttaaaaac ggctgttcag tcctttatgt
      721 cgcaggttat tgcaacaatc atccggtgat tgtcgggagg aatatcaatg cttccgttaa
      781 tttttactgg ctgataaagt tttgtctgct gaatctttat acggaattcc tttcagtatg
      841 cgtacacgaa ttgactatct tgcagataaa tacagtttta ctgaactgaa tgaacgtcca
      901 caccttcgcc ggcagtggca ggatgttctg gaggagtgtc ggcagacaga ggccgggcca
      961 gaagaacggc tgcggattgc cctgttgaat gtggattacg tcaccagttt tgaacttcca
     1021 tttcggttgc tgcttatccg tacaccacaa ctgattgctg cgttgcgtga agaatgggac
     1081 atcggccaga aaaatgtgct gtttaacgat aaacggtttg gttgcgtgta cagcctgaag
     1141 tccagtcttt ctggtgtacc ggatacatac cggtatcatc tgtctcaccg tattcgtcgt
     1201 gtggttgcga atgaaaacac atcattgcca tatcagcaga ttgcccggga agtgaaagcc
     1261 ccccgggaac gactgaagta tgcgctggag acgggattgc aggtgaccgc actggacggc
     1321 ttgttctggt tcggtagcca gcgcattgct gcagatatac agagactgag aaaatctggt
     1381 atgaggatta ccacttctgc cataagcgtg tatgactcta ttaccgtaac ccggagattg
     1441 tgcccggtat accggattgt cgcgaataat aaataaacgg taatgctgag tttctccatt
     1501 accattgcaa atgactcacc agagcagact gaacagcgca gaagtgggat tgtggatacg
     1561 tgaagtgaga gtaaggggaa aatccacaat aatcatctat cgaacaggga ggcgaacttt
     1621 acacgatggt tttccgggag tgcttacccg gggttcctca cctctggcta atctctggat
     1681 tgagtcgcga tactccaaca aaagcaacaa gctaacgcag caagaagtta acgctcatcg
     1741 agagtaaaat gcacactttt atggcttact cgttacaata acagccagtt tgttcagaaa
     1801 accggattca gtatggccag aataccaacc aaaaaagcta aagcaaaaaa agggtttgaa
     1861 gaaacattat gggatgccgc aaatcagctt cgcggcagcg ttgagtcctc cgaatacaag
     1921 cacgtggtgt tgagcctcgt gttcctgaaa ttcatcagcg ataagtttga aacacgccgc
     1981 aaaaaaatga ttgccgatgg gcaggcagat ttccttgaga tggaagtgtt ctaccagcag
     2041 gacaacattt tctacctgcc ggaagaggcg cgttggtcat ttatcaaaca aaatgcaaaa
     2101 caggacgata ttgcggttcg tattgacacc gccctctcga ccattgagaa acgtaaccca
     2161 accctgaaag gtgcgctgcc agacaactac ttcagccgtc agaatctgga aaccaaaaaa
     2221 ctggcatcac tgattgatac catcgacaac atcgaaacgc tggcacacga gactgacgtt
     2281 gaaacgttat cgaaagaaga cctggtcgga cgcgtttatg aatacttcct cggtaagttt
     2341 gccgccactg aaggcaaagg cggtggtgag ttctacacgc caaaatgtgt ggtcacgctg
     2401 ttaactgaaa tgctcgaacc cttccagggc aaaatttatg acccgtgctg cggctcggca
     2461 ggaatgttcg tgcagtcggt gaagtttgtc gagagccatc agggtaaaag ccgtgatatc
     2521 gcgctgtatg gtcaggagct gacagccacg acgtataaac tggcaaaaat gaacctcgct
     2581 attcgcggtc tttcagctaa cctcggcgaa cgcccggcaa acactttctt tagcgaccag
     2641 cacccggacc tgaaagctga ctatattctg gcgaacccgc cgttcaacct gaaagactgg
     2701 cgtaacgaag cagaattaac caaagaccca cgttttgccg gttatcgtat gccgccaacc
     2761 ggtaacgcca actacggctg gattttgcat atgctctcca agctgtcggc taacggcaca
     2821 gcgggttttg tgctggcaaa cggttcgatg agttctaaca ccagcggtga aggcgagatc
     2881 cgtgcacaga tgatcgaaaa tgatctgatc gactgcatga ttgctctgcc aggtcagttg
     2941 ttttacacca cgcagatccc ggtgtgttta tggtttatga ccaaatcgaa ggctgccgat
     3001 ccggccaaag gttatcgtga tcgtcagggc gagacgctgt ttattgatgc gcgtaacctc
     3061 ggcaccatga ttagccgcac aactaaagag ttaacagcgg aagatattgc cacaatcgcc
     3121 gatacttacc atgcctggcg tagcacgcca gaagaactgg ctgcacggat tgcgcgtggt
     3181 gacagcaagc tggaaaaata tgaagaccag gcaggcttct gcaaagttgc gaccctgcaa
     3241 gatattaaag ataacgacta cgttctgaca ccgggccgct atgtgggtgc agccgagcag
     3301 gaagaagacg gcgtggcatt tgagaccaaa atgcgtgaat tgtcgaagac gttgtttgag
     3361 cagatgaagc aggcggaaga actggatcgt gcgattcgcc agaatctgga ggcgctgggt
     3421 tatggggagt aaatgggaga aaataaaact t
//
LOCUS       DAZKFZ010000309         3445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000309 DAZKFZ010000000
VERSION     DAZKFZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..839
                     /gene="yibB"
                     /locus_tag="ACHOQW_004627"
     CDS             3..839
                     /gene="yibB"
                     /locus_tag="ACHOQW_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFP9575542.1"
                     /translation="MTAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMI
                     IFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKN
                     PEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFD
                     ESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKIT
                     LNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLS
                     RK"
     gene            1114..2310
                     /gene="kbl"
                     /locus_tag="ACHOQW_004628"
     CDS             1114..2310
                     /gene="kbl"
                     /locus_tag="ACHOQW_004628"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9575543.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANVRQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2320..3345
                     /gene="tdh"
                     /locus_tag="ACHOQW_004629"
     CDS             2320..3345
                     /gene="tdh"
                     /locus_tag="ACHOQW_004629"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9575544.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
BASE COUNT          868 a          738 c          909 g          930 t
ORIGIN      
        1 ttatcactgc atattttgac attggtagag gggattggac tgctaataaa gggttccgtg
       61 aaaaactagc tcgttcagtt gatgtttatt ttagttactt tgagcgtctg gccgcacttg
      121 aaaatgagat gattattttc acttctcccg atttgaaacc aagggttgaa gcgattcgca
      181 acggaaaacc aacaacggtt atcgttatcg atataaaaaa gaaatttaga tatatcagaa
      241 gtcgtatcga aaaaattcaa aaagatgagt cgtttacaaa tagacttgag cctcgacaat
      301 taaaaaaccc agagtactgg tcaccagagt atgttttggt atgtaacctt aaggcctatt
      361 ttgtaaataa agctatcaac atgggcttag tgaaaacgcc tcttgttgcg tggatagatt
      421 ttggctattg ccgtaagcca aatgtaactc gaggattgaa aatttgggat ttcccatttg
      481 atgaaagtaa gatgcatctt ttcactataa aaaaagggct tactgtcaca tctcaacaac
      541 aggtttttga tttcatgatt ggcaatcatg tatatattat tgggggcgca attgttggtt
      601 cacagcataa atggaaagaa ttctataaat tagttctgga aagtcaaaaa ataacactaa
      661 ataataacat tgtggatgat gaccagggca tttttgtcat gtgctattac aaacgccccg
      721 atctcttcaa cctaaactat ctggggagag gaaaatggtt cgatttgttt cgctgcttca
      781 ggagtaacac tttaggggca aaaatgcagg cgctgagaat tttcctttca agaaaatagg
      841 tttccctcca ttagtgggta gcgttttgat accaactgag ataggaaaaa tgatgctttg
      901 gcatcatttt ttctttttat atcaatgcag taaaatcaat catcattatg cccagccaaa
      961 actgtacagt ttttctacta tctgtaggtg ttgagagaca tcaagttaca cgtcatttat
     1021 cctgaatttt gcagaagagt taacgcgtca tctcgtcgcg acctataagt ttgggtaata
     1081 tgtgctggaa tttgccctgt ctggagaatc gcaatgcgtg gagaatttta tcagcagtta
     1141 accaacgatc tggaaaccgc acgggcggaa gggttgttta aagaagagcg cattattacg
     1201 tctgcgcagc aagcagatat cactgtggct gatggaagcc acgtcattaa cttttgtgcc
     1261 aacaactatc tcgggctggc gaatcatccg gatctgattg cggcagcaaa ggcgggaatg
     1321 gattctcacg gtttcggcat ggcttcggtg cgttttattt gcggcactca ggacagccat
     1381 aaagagcttg aacaaaaact ggcggcattc ctggggatgg aagatgcgat tctctactct
     1441 tcctgctttg atgctaacgg tggactgttt gaaacgctgc tgggtgcgga agacgccatt
     1501 atctccgacg cactgaacca cgcgtctatt attgatggtg tgcgtctgtg caaagctaaa
     1561 cgctatcgct atgccaacaa cgatatgcag gagctggaag cacgtctgaa agaagcgcgt
     1621 gaagccggtg cgcgtcatgt gctgattgcc accgatggtg tgttctcaat ggacggcgtg
     1681 attgccaacc tgaaaggcgt ttgcgatctg gcagataaat acgatgcctt ggtgatggtg
     1741 gatgactccc acgcggtcgg ttttgtcggt gaaaatggtc gtggttccca tgaatactgc
     1801 gatgtgatgg gccgggtcga tattatcacc ggtacgcttg gtaaagcgct gggcggggct
     1861 tctggtggtt ataccgcggc gcgcaaagag gtggttgagt ggctgcgcca gcgttctcgt
     1921 ccgtacctgt tctccaactc gctggcaccg gccattgttg ccgcgtccat caaagtactg
     1981 gagatggtcg aagcgggcag cgaactgcgt gaccgtctgt gggcgaacgt gcgccagttc
     2041 cgtgagcaaa tgtcggcggc gggctttacc ctggcgggag ccgatcatgc cattattccg
     2101 gtcatgcttg gcgatgcagt agtggcgcag aaatttgccc gtgagctgca aaaagagggc
     2161 atttacgtca ccggtttctt ctatccggtc gttccgaaag gtcaggcgcg tattcgtacc
     2221 cagatgtctg cggcgcatac ccctgagcaa attacgcgtg cagtagaagc attcacgcgt
     2281 attggtaaac aactgggcgt tatcgcctga ggatgtgaga tgaaagcgtt atccaaactg
     2341 aaagcggaag agggcatctg gatgaccgac gttcctgtac cggaactcgg gcataacgat
     2401 ctgctgatta aaatccgtaa aacagccatc tgcgggactg acgttcacat ctataactgg
     2461 gatgagtggt cgcaaaaaac catcccggtg ccgatggtcg taggccatga atatgtcggt
     2521 gaagtggtag gtattggtca ggaagtgaaa ggcttcaaaa tcggcgatcg cgtttctggc
     2581 gaaggccata tcacctgcgg tcactgtcgc aactgtcgtg gcggtcgtac ccatctgtgc
     2641 cgcaacacca ttggtgtcgg tgttaaccgt ccgggctgct tcgccgaata tctggtgatt
     2701 ccggcgttca acgctttcaa aatccccgac aatatctccg acgacctggc ttccattttt
     2761 gatcccttcg gtaacgccgt gcataccgcg ctgtcgttcg atctggtggg cgaagatgtg
     2821 ctggtttctg gtgcaggtcc gataggtatt atggctgcgg cggtggcgaa acacgttggt
     2881 gcacgcaatg tggtgatcac cgatgttaac gaataccgcc ttgagctggc gcgcaaaatg
     2941 ggtatcaccc gtgcggttaa cgtcgcgaaa gaaaacctta atgatgtgat ggctgaactg
     3001 ggcatgaccg aaggctttga tgtcggtctg gaaatgtccg gcgcgccgcc agcgtttcgt
     3061 accatgctcg ataccatgaa ccacggcggt cgtattgcga tgctgggtat tccaccgtct
     3121 gacatgtcta tcgactggac caaagtgatc tttaaaggct tgttcattaa aggcatttac
     3181 ggtcgtgaga tgtttgaaac ctggtacaag atggcggcgc tgattcagtc tggcctcgat
     3241 ctctcgccga tcattaccca tcgtttctct atcgatgatt tccagaaggg ctttgacgct
     3301 atgtgttcgg gccagtccgg gaaagttatt ctgagctggg attaacacga aaaagggctg
     3361 gtattccagc ccttttgcct gaggataatc tgttaaatat gtaaaatcct gtcagtgtaa
     3421 taaagagttc gtaattgcgc tgatc
//
LOCUS       DAZKFZ010000310         3435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000310 DAZKFZ010000000
VERSION     DAZKFZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..163)
                     /gene="escO"
                     /locus_tag="ACHOQW_004630"
     CDS             complement(<1..163)
                     /gene="escO"
                     /locus_tag="ACHOQW_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFP9575545.1"
                     /translation="MLDRILSIRKSRANRLRESMAKINSQIKEVDGKLDDCEQSIKES
                     IASKQAYCAS"
     gene            complement(166..1506)
                     /gene="escN"
                     /locus_tag="ACHOQW_004631"
     CDS             complement(166..1506)
                     /gene="escN"
                     /locus_tag="ACHOQW_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFP9575546.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1490..3433)
                     /gene="escV"
                     /locus_tag="ACHOQW_004632"
     CDS             complement(1490..3433)
                     /gene="escV"
                     /locus_tag="ACHOQW_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFP9575547.1"
                     /translation="MMIIPLPTVVVDIIIAINISTALLLLMLSIYIKNPLELTSFPTI
                     LLITTLMRLSLSVSTTRLILLHHDAGDIIYSFGNFVVGGNIVVGLVIFTIITIVQFMV
                     ITKGAERVAEVSARFSLDGMPGKQMSIDGDMRAGVIDPLEAKVLRSRVQKESQFYGSM
                     DGAMKFVKGDAIAGIIIVLVNLFGGVLIGMWQFDMPFSAALSLFSVLSVGDALVAQIP
                     ALIISVTAGVVVTRVPGESEKEENLAGDIVQQVSVNSRPFLISAALMLVMAIIPGFPA
                     LVFLFLAVCLLGIAWKLQKKRTFGTGNNKDAMGADLSNSQNISPGAEPLILNLSSNIY
                     SSDITQQIEVMRWNFFEESGIPLPKIIVNPVKNNDSAIEFLLYQESIYKDTLIDDTVY
                     FEAGHAEISFEFVQEKLSTNSIVYKTNKTNQQLAHLTGMDVYATTNDKITFLLKKLVL
                     SNAKEFIGVQETRYLMDIMERKYNELVKELQRQLGLSKIVDILQRLVEENVSIRDLRT
                     IFETLIFWSTKEKDVVILCEYVRIALRRHILGRYSVSGTLLNVWLIGSDIENELRESI
                     RQTSSGSYLNISPERTEQIIGFLKNIMNPTGNGVILTALDIRRYVKKMIEGSFPSVPV
                     LSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT         1039 a          815 c          640 g          941 t
ORIGIN      
        1 gcgatgcaca atatgcctgc ttagaggcaa tcgactcttt aatggattgc tcgcagtcat
       61 cgagtttacc atccacctct tttatctgag agttaatctt agccattgat tctctcaatc
      121 ggttcgctct gcttttacga atagataaaa ttctgtccaa catactcagg caaccacttt
      181 gaataggctt tcaatcgttt tttcgtaact actgatatct ttggtgcttt gttgtataaa
      241 gttctgaatt aatttcctat tttttattgc cttatctgct tcaggatctt gccccatcgt
      301 gtactcacca atacgaataa gcagctcaac atttttataa gttgcaataa gctttttgca
      361 ttctgccgcc gcacgcaaat gctccgatgt aacaacgtta tgcataacac gactggcact
      421 taaaccaata tcaattgcag ggaaatggtt ttcctctgca agttctcggg taagtacgat
      481 gtgcccatca agaatagagc ggacttcatc gccaatagga tcattaacat tatcgctttc
      541 taacaacacc gtataaatcg cggttattga accttttggc gcaggccccg ctcgctctaa
      601 taatttgggt aacgaagaaa aaacactcgg agggaatccc ccccttacat caggttctcc
      661 cgatgcaagc ccaacatcac gtgcggctcg ggcataacga gttacagaat ccatcattaa
      721 taatacattt ttaccttgat cgcggaagta ctctgcaata gtcgtcgccg taaatgcggc
      781 tttcatcctt tccagcgcgg ggcggtctga cgttgtgacg accagtacac atttagaaag
      841 cgtggattga ggtaagagcg cgaggaattc atttacttcg cgaccacgtt cgccgataag
      901 agcaaggaca ataatatctg cggatgcgcc attacaaatc atccccaaaa gcgtactttt
      961 accaacgcct gaaccagcaa aaataccgat acgctgccca ataccgcaag ttagtagccc
     1021 atcaattgcc ctgacgccaa gtataaatgg ctgatcaata acctgcctta ataaaggatc
     1081 tggtggttca gcatacaagc tgcgttcaaa cggaagatag ggggcagcaa tattactctc
     1141 cattggtctg cctatgccat caacaagccg cccaagtagt gcgtctccaa cacgaatctt
     1201 gaactcatct ccctgataac ttaaccactg accacagtac atgccggaaa catgctcaaa
     1261 aggcaacaag aaaacctcat cttcatcaat agcgatcacc tcggctaaac gttgactggg
     1321 ttctatctta taaaaggcgc caatacgcgc ttttggtaaa cgcgccttaa taatcgtccc
     1381 gccgatattg ataatcttgc cttcatatct ggtagacgaa tttaatgata atgccggcac
     1441 agtgctattg agcactttct gaatacgtgg gtatttttcc aatacagaat catgctctga
     1501 aatcatttac cgttcctaat actttaagtt cgatattatt cccaacctcc tgaaaagaga
     1561 gcacggggac tgacgggaac gaaccttcaa tcattttctt cacatagcgc ctgatatcta
     1621 aagcggtcag aatgacgccg ttccccgttg gattcatgat attttttaag aagccaatta
     1681 tctgctcagt tcgctccggt gagatattca gatacgaacc tgatgacgtt tgtctgatcg
     1741 actctcgtag ctcattttca atatcagagc caataagcca aacgttcaga agtgtaccgc
     1801 taacgctata gcgacctaaa atatgccgac gcagggcaat acgaacgtat tcgcacaaga
     1861 taaccacatc cttttctttt gttgaccaaa aaataagcgt ctcaaagata gttctcaggt
     1921 ctctaattga gacattttcc tctacgagac gttgtaggat gtcaacaatt ttgctcaaac
     1981 caagctggcg ctgcagctct ttcacaagct cgttatattt tctctccatg atgtccatca
     2041 aataacgcgt ttcttgtacg ccgatgaact ctttggcatt agacaataca agtttcttta
     2101 gcaaaaacgt tatcttatca tttgttgttg cataaacatc catacctgta aggtgagcga
     2161 gctgttgatt agtcttattc gtcttatata cgatagagtt agtcgaaagc ttttcctgga
     2221 caaattcgaa tgatatctct gcatgcccag cctcaaaata gacagtatca tctataagag
     2281 tatctttgta tattgactct tgatagagca aaaattctat tgcgctatca ttatttttaa
     2341 ccggattaac aataatctta ggcaatggaa ttccactttc ctcaaagaaa ttccaacgca
     2401 tgacctcaat ttgctgtgta atgtctgaac tataaatatt actacttaag tttaaaatca
     2461 atggctcagc gcccggtgag atgttttggc tattagacaa atcagctccc atagcatcct
     2521 tattattgcc agttccaaat gttcttttct tctgtaattt ccaggctatc cccaacaggc
     2581 aaaccgccag aaataagaag accaatgcag gaaagcccgg aataatcgcc attacgagca
     2641 ttagtgcggc actgattaaa aaagggcgac tattaacaga aacctgttga acaatatcac
     2701 ctgcaaggtt ttcttctttt tcgctttcac cgggcacacg agtaacaacc acgcctgcgg
     2761 tgacagaaat aataagcgca gggatctggg caaccaaagc atcaccgaca gacaatacag
     2821 aaaacaggct aagtgccgca ctaaatggca tgtcaaattg ccacatacca atgagcacgc
     2881 caccaaaaag gttaactaaa acaataatga taccagcaat agcgtcccct tttacaaact
     2941 tcatcgcccc gtccattgaa ccataaaact ggctttcctt ttgcactcgg gagcgaagaa
     3001 cttttgcctc aagcgggtca ataacgcctg cgcgcatatc accatcaata ctcatttgct
     3061 ttcctggcat accatcaagg gaaaaacgag cactcacttc agcaacacgc tcggcccctt
     3121 ttgtaataac cataaattga acaatggtga taatagtaaa aataaccagg ccaacaacaa
     3181 tattaccgcc aacaacaaag ttaccgaagg agtaaataat gtcacccgca tcgtgatgca
     3241 gcagaataag tctggttgta ctaacactga gcgacaggcg catcaacgtc gtaatcaaca
     3301 ggattgtggg gaaagaagtt agttcaagag gattttttat atagattgag agcattaata
     3361 aaagtaaagc tgtcgaaata ttaatcgcga taatgatatc caccacaact gttggtagtg
     3421 gaataatcat catca
//
LOCUS       DAZKFZ010000311         3411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000311 DAZKFZ010000000
VERSION     DAZKFZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(190..909)
                     /gene="dcuR"
                     /locus_tag="ACHOQW_004633"
     CDS             complement(190..909)
                     /gene="dcuR"
                     /locus_tag="ACHOQW_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP9575548.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(906..2537)
                     /locus_tag="ACHOQW_004634"
     CDS             complement(906..2537)
                     /locus_tag="ACHOQW_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9575549.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2718..2948
                     /gene="yjdI"
                     /locus_tag="ACHOQW_004635"
     CDS             2718..2948
                     /gene="yjdI"
                     /locus_tag="ACHOQW_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP9575550.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2960..3232
                     /locus_tag="ACHOQW_004636"
     CDS             2960..3232
                     /locus_tag="ACHOQW_004636"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9575551.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          878 a          775 c          851 g          907 t
ORIGIN      
        1 gttaattaac ttttgttagc gttttgaaat taaaaacacc gttcacctga agagatatta
       61 atttttagcg atgatggagg gataattata tttgatctgg cacaagtttt actgatgaag
      121 gatgtaactt gtaccagggg tattttgcat tacggtaata attattactt acagataacc
      181 agcgatccgt tattggcaat attgtttcag tagtgagtag tgttctgcct gaatacggta
      241 acggtaaact ggacgccccg tgacgccata atggatactg gtgaacaaga tgtggcagtt
      301 gaccagccag ataaggtatt tacggcagga aacacgcgaa atgttaacct cgttggctag
      361 ctcgtcggtt gaaaactcat agtcctgatg cgcgtcaatc cactggcaca gtgtgcgtaa
      421 cgtctgcggc gttaagcctt ttggcaagcg acgaggatcc tgctcgttgg agctgctgcc
      481 gtggattagc tgatcaagct cggcctggtc ataatactga tgtttttcca gcgccatttt
      541 cttttgccgc cagccggtga gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag
      601 gtaatccacg acaccgtaat gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga
      661 gatgacaatc acatcacttt tgcaacgcgc gttatgcagg acaggcagta aatcgagccc
      721 gttctctttt tgcatataga tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa
      781 gataatctct ttggctttct ccagcgtcga ggctgttcca cagcattgaa agcctgggat
      841 ttgtgctacg tatcggcgat tcagctccgc gaccattgcg tcgtcatcga taattaatac
      901 attgatcatc tgttcgacct ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa
      961 atcccgggtt ccgattccac ggcgatgctg ccgccgagat tttctacctg ttgtttgaca
     1021 agtgctaaac cgacgcctcg ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga
     1081 tcgattttgt cgggtgcgat ccccggtcca tcatcgttaa cttcgcagtg cagccagccg
     1141 tgacggtagt gcaatgttac gctaatttcg ccaccgggtt ccggccctaa tgcttccagc
     1201 gcgttttcta tcagatttcc caatgtggta atcagcgtcg cgacctggtc ctcactgccg
     1261 ctgtctggca gctggctttc actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcgg
     1321 ttaatcttgc tgattaaaaa accagcgata accggagatt tgatcttacc cagcagagag
     1381 ccaatctctt cctgatagtt attggctgtt ttgagaatgt aatcttccaa ctgcttataa
     1441 ctcttcagat gcaataatcc gagaatcaca tgcaatttat tcataaattc gtgggatcgt
     1501 tcacgaagtg catcagcata gttgaccaga ccgtcgagtc gctgcatcag tttacgaact
     1561 tcagttttgt ccctgaaggt tgaaatggca ccgatgataa cgccattact gcgcaccgga
     1621 acggtgttga tcagtaatag ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta
     1681 ccgtcgcgta acacttccga gacatctacc acctgtgacc atgagtggct gagcgtcgac
     1741 agtttctcat cgtcctgcga cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc
     1801 agcgtgacct cgccgcgatc gtccacggca acgacgcctt ctttgataga ctgcaacatg
     1861 gcctggcgtt gctcaaacag cgtggagatt tcgtagggtt ccaggccgaa aaggattttt
     1921 ttcagtacct taaccagaat gcaggtgcca atcagtccga ccagcatgcc aaataatacc
     1981 gaccagataa tgctccagcg actgtcattg atctgttggg tcacgcggct taactcaagg
     2041 ccgatcgcca ccacgccaat ttgtttatga ttttcatcgt agatgggggt aaatacgcgt
     2101 aaagcctgcg ccagaaaacc gcgattgata gcgacatttt cttcgccatt cagcgcttta
     2161 aggatgtcat cacctttaaa tggctgacca atacgctggg cttcaggatg cgagtagcga
     2221 agactttgca tatcggtaac gacaataaac agcagatcgt tgcgtttgcg cacggcttcc
     2281 gcgatggcct ggatgccgct ctcctgcggt tttttctgca agccctgacg gatttccggc
     2341 gagtcggcga gagtacgcgc cactgccagt gccttgttgg ctagcccgtc tcgcgtcata
     2401 tcactgattt gcgagaagta aatcagatgc accaccaata gcaccgagaa cagtaccgcg
     2461 ctgaccatta agatcactgt ggtactcaat ttcatcggac gtttgcgtaa catgcggtag
     2521 ggcaatgaat gtctcatcag cttccttgtg tgacaaattt cttaagcatt atctctgatg
     2581 aggcgggtaa ttcaaaggga gtaagaatga ttggctatat aggggaagag actctggcaa
     2641 cggaaactgc cagtgctgta tgaagattcc agggctatgc ttatagtgaa aaccatactg
     2701 atgagagagg gaaggtcatg gatcaggcgc tactggacgg gggttatcgc tgttataccg
     2761 gcgaaaagat cgatgtctat ttcaacactg cgatatgtca gcattctggc aattgcgtac
     2821 gtggcaacgg caagttattt aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg
     2881 acgttgccac tgtggttaaa gtgattgata cgtgcccgag tggtgcgctg aaataccgtc
     2941 ataaataagc gagggtaaaa tggaaatacg cgaaggccac aataaatttt acattaatga
     3001 cgaacaaggc aagcaaatcg ctgaaattgt ctttgtgccg accggagaga atttagcgat
     3061 tatcgaacat accgatgtcg atgaaagcct gaaagggcaa gggattggta aacagctggt
     3121 tgcgaaagtc gtggaaaaaa tgcgtcggga aaaacgaaaa attatcccat tatgtccatt
     3181 tgcgaaacac gaatttgata aaacgcggga gtatgatgat attcgcagtt gatggaagag
     3241 tacagattca cgatattttt catgctctcc gcggtgtgat gcaggagagc atctgaaggg
     3301 tagggggatg cacaaagaat gggcagagag cgcgtttttt tgtcccaagt catccccttt
     3361 actgagcaaa aaaaagaata tctcctatat gagaatcatc aatcggggtt a
//
LOCUS       DAZKFZ010000312         3367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000312 DAZKFZ010000000
VERSION     DAZKFZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..903)
                     /locus_tag="ACHOQW_004637"
     CDS             complement(<1..903)
                     /locus_tag="ACHOQW_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9575552.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSAV"
     gene            complement(970..3078)
                     /locus_tag="ACHOQW_004638"
     CDS             complement(970..3078)
                     /locus_tag="ACHOQW_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9575553.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          694 a         1019 c          890 g          764 t
ORIGIN      
        1 caccgccgaa aggcggatac ctctgtcggg accgtattcg gtaccgggca gtgtgtcggg
       61 gaacgctgag tctgaaagag ggcggggggt gtcaggggaa gataaagaag cggtgtgctg
      121 tttacgggcc tcttccgccc gctgcgcctg cgtggtcagc accagacgga actcccgccc
      181 ggcaccgtcc gtcacgccgg tgatttcccc ggccaggtca ccggcggcct cacgcctgta
      241 cgtcagcgtc cgcccgaagc ggtccgccat cccggtaagc acccggtacg gcggcagcgg
      301 cgctggcagt acgtcctcag cacccggcac ccgctctgac caccccagta tccaccacgg
      361 cccctgtgcg ctgttggtcg ccaggtaaag atgcgggctt aaccggatat ccggcggcag
      421 cgccccccac agccgcgcca gcgtgtggcc gtccggctgc gctgccttac caccgcgcac
      481 cagccacatt gactcgctgc ggctgtacac cgcctccccc ggcagcagcg gctcaaagtg
      541 aatgctccgc ccgccgttgt cattgagtac cagtgcatca tcgcgtagct gtaagcggat
      601 atcagaaggc gctttccagc cggggccaaa aatccccacc ggcgcaggcg tccgggtccg
      661 gtagctgctg taggtgcggg agagaatgaa cggcagcggg ccgggcagcg caaggtccgt
      721 ctcgccgggc agcaccttcg cccccagcag cggatttacc gggttgcccg aagtcatccc
      781 gcccgggcac accgagcacg ccacgccagt tggcgcgcca attcttacac ctgccgaacc
      841 ctggacaatg ggaccgccat actgagtcat atctccctga cgcgccgctg gttttccgct
      901 catgattcaa ttccttcttc tgtttatttt taatgcttgt ttgttactga ataccgaaca
      961 ggtacgggat cagtactctc tcatatccgg cgtatcctgc ttttcatcag gggtcttcgg
     1021 tggggatttc gaggctgcac agttccagtt aatcaacagt gagtcaccat taacctgctc
     1081 cttaccctgc aaatgaattt ttgtgccgtt gagaaaaatt tgtccgtcct tcgtcagcac
     1141 cagccttgcc ttaccacagc agagctccag atgctcaccg gcggagtaaa tcgcggtctg
     1201 cccggtatca tcctttttcg ttttaccaac ggtgaaggta acgttatttc cgactttgac
     1261 gtcataaccc agcccgaccc tcaacgattt atgcaaaccg atctgcgagg actgcatcag
     1321 agccaccgaa gtgttcataa tgccacctac cgtcgtctgg tacgccacgc ccacggtcag
     1381 cgcacgaaca agtccaatcg tttccacctg aacagaaccc acgttaatga tctggttact
     1441 gcccaccgtc tctatctggt taacgcccac cgtctgtatc tgattgttgg taaccgcaat
     1501 catctgattg ttgccaatgg tctcagcatg gttgtttatc acatccgtgg tgcggttatt
     1561 cagcacctcg gtgttcatgt tcttctgcgc gtggatgtag acctgttctt tccctgtcgc
     1621 atcgtcaaac ttcagttcat taaacccgct gcccttatag tttttcgaac gaatggtcat
     1681 ctgcgtcttc gtccccggca ggctgccggg tgtgcggttt tcctggtggt aggtacgccc
     1741 cataataatc ggctggtccg gatcgccgtt gaggaagtcc acaatcacct cctgacccac
     1801 acgcggtatc gccaggttgc caaagccggt gcctgcccac gcctgtgcca cacggatcca
     1861 gcatgaactg tcctggtttg acgggttata gcggtcccag ttaaatttca cccgcacgcg
     1921 accatgttcg tcgcagaaga tttcctcgcc tgccggtccc gtcacgacgg cgctctgcgg
     1981 gccatccacc agcggtttca gcaacggctg tggtcgccat gtccggtctg ccggtattac
     2041 cgcaaagtgg ttattcagcg tggtacctga acccctgcgt cccggcaccg cctgtggctg
     2101 ctcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg cctgcggatg
     2161 ccccgtcagc acaattcgcc gtcccggcca tatttccgga gaacggcttg ttccgcgcgc
     2221 cacttctgcg ttgttgcgcc agccatccat ctgccagcgg gcaaagttct gcccgtgggc
     2281 acccttgaaa cgtccggggt agtcatacac ttcatactgt gtgcgctggt aatcctggta
     2341 ctggccttcc tgatcaaaac gccctgccca gccgggtcgt ttaaaggtgt agtccttggt
     2401 caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc atagggtgct
     2461 cacctcggta cgggtgttcg ggttccaggg gatctcaaag gactccggca gataacgcac
     2521 ggtgtcgcac aggaccaggc tctggtcgat acttttttgc gcgtgctcct cataaaagaa
     2581 gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtttcgc cgtactggac
     2641 acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccactcgg tcacaccgtt
     2701 ttctttcagg atcgtagcga gtatgctttc gatgtcctca ttctggaaga tacggaagtt
     2761 ctggcgcagc cctgtgcgcc acagcggcgg gcacaccttc atgctgtaca gcatctggtt
     2821 tttgtcgttc tcccccagtt caaaccaggt caccacacct ttcacccggc gctgtacgtc
     2881 atcgccctgc cataccgtca ggtaggccat tttgtccagc acctgcgcaa attcaaggga
     2941 aagaaactgc tgactgacca gagaaaggtc gagggaaaaa agtgaagaga gtgactggtt
     3001 cagatgaaag gagaccaccg caaacgcgtc cggtggcagg ccgtccactt ccagcgtgaa
     3061 acgtaatccg gttgacatag caacctccgt gaaaatgaat aataatccgc acatattgca
     3121 tgtacaacat gtcgggcgga tgaaataagt gagtcgacat ctggcgttac cggggaatat
     3181 cagaaaaatg tcatggttat gtgatgattt agtctgggat tttacttcag agaaacgggt
     3241 gtctgtttag tcaacaaaat aatcacaaac gcaacggatg ggttctcgac gttattcacg
     3301 acaggctcgt acattacagc gagtaataaa aaacctgata tgcaaagtca aaccaatatt
     3361 cataatg
//
LOCUS       DAZKFZ010000313         3358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000313 DAZKFZ010000000
VERSION     DAZKFZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..2800
                     /gene="eae"
                     /locus_tag="ACHOQW_004639"
     CDS             <1..2800
                     /gene="eae"
                     /locus_tag="ACHOQW_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFP9575554.1"
                     /translation="THGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENYFK
                     LGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMMKA
                     APGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDDKA
                     LNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQSG
                     NNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNVFI
                     DQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNG
                     YLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHG
                     TGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQD
                     ILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQDY
                     QAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTADKT
                     SAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTLKS
                     STPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTVKV
                     MKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVSDG
                     AEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYSEN
                     TSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMSIC
                     KNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLITQN
                     PLPGVNVNTPNVYAVCVE"
     gene            complement(3064..>3358)
                     /locus_tag="ACHOQW_004640"
     CDS             complement(3064..>3358)
                     /locus_tag="ACHOQW_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscD/YscD/HrpQ family type III secretion system
                     inner membrane ring protein"
                     /protein_id="HFP9575555.1"
                     /translation="SQQNESKILAIINAMNKNSNAKILFQNIQPYISADIFPGKILRI
                     SGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT          983 a          659 c          753 g          963 t
ORIGIN      
        1 tactcatggt tgttataccc ggacccggca caagcataag ctaaaaaaaa cattgattat
       61 gcttagtgct ggtttaggat tgttttttta tgttaatcag aattcatttg caaatggtga
      121 aaattatttt aaattgggtt cggattcaaa actgttaact catgatagct atcagaatcg
      181 ccttttttat acgttgaaaa ctggtgaaac tgttgccgat ctttctaaat cgcaagatat
      241 taatttatcg acgatttggt cgttgaataa gcatttatac agttctgaaa gcgaaatgat
      301 gaaggccgcg cctggtcagc agatcatttt gccactcaaa aaacttccct ttgaatacag
      361 tgcactacca cttttaggtt cggcacctct tgttgctgca ggtggtgttg ctggtcacac
      421 gaataaactg actaaaatgt ccccggacgt gaccaaaagc aacatgaccg atgacaaggc
      481 attaaattat gcggcacaac aggcggcgag tctcggtagc cagcttcagt cgcgatctct
      541 gaacggcgat tacgcgaaag ataccgctct tggtatcgct ggtaaccagg cttcgtcaca
      601 gttgcaggcc tggttacaac attatggaac ggcagaggtt aatctgcaga gtggtaataa
      661 ctttgacggt agttcactgg acttcttatt accgttctat gattccgaaa aaatgctggc
      721 atttggtcag gtcggagcgc gttacattga ctcccgcttt acggcaaatt taggtgcggg
      781 tcagcgtttt ttccttcctg caaacatgtt gggctataac gtcttcattg atcaggattt
      841 ttctggtgat aatacccgtt taggtattgg tggcgaatac tggcgagact atttcaaaag
      901 tagcgttaac ggctatttcc gcatgagcgg ctggcatgag tcatacaata agaaagacta
      961 tgatgagcgc ccagcaaatg gcttcgatat ccgttttaat ggctatctac cgtcatatcc
     1021 ggcattaggc gccaagctga tatatgagca gtattatggt gataatgttg ctttgtttaa
     1081 ttctgataag ctgcagtcga atcctggtgc ggcgaccgtt ggtgtaaact atactccgat
     1141 tcctctggtg acgatgggga tcgattaccg tcatggtacg ggtaatgaaa atgatctcct
     1201 ttactcaatg cagttccgtt atcagtttga taaatcgtgg tctcagcaaa ttgaaccaca
     1261 gtatgttaac gagttaagaa cattatcagg cagccgttac gatctggttc agcgtaataa
     1321 caatattatt ctggagtaca agaagcagga tattctttct ctgaatattc cgcatgatat
     1381 taatggtact gaacacagta cgcagaagat tcagttgatc gttaagagca aatacggtct
     1441 ggatcgtatc gtctgggatg atagtgcatt acgcagtcag ggcggtcaga ttcagcatag
     1501 cggaagccaa agcgcacaag actaccaggc tattttgcct gcttatgtgc aaggtggcag
     1561 caatatttat aaagtgacgg ctcgcgccta tgaccgtaat ggcaatagct ctaacaatgt
     1621 acagcttact attaccgttc tgtcgaatgg tcaagttgtc gaccaggttg gggtaacgga
     1681 ctttacggcg gataagactt cggctaaagc ggataacgcc gataccatta cttataccgc
     1741 gacggtgaaa aagaatgggg tagctcaggc taatgtccct gtttcattta atattgtttc
     1801 aggaactgca actcttgggg caaatagtgc caaaacggat gctaacggta aggcaaccgt
     1861 aacgttgaag tcgagtacgc caggacaggt cgtcgtgtct gctaaaaccg cggagatgac
     1921 ttcagcactt aatgccagtg cggttatatt ttttgatcaa accaaggcca gcattactga
     1981 gattaaggct gataagacaa ctgcagtagc aaatggtaag gatgctatta aatatactgt
     2041 aaaagttatg aaaaacggtc agccagttaa taatcaatcc gttacattct caacaaactt
     2101 tgggatgttc aacggtaagt ctcaaacgca agcaaccacg ggaaatgatg gtcgtgcgac
     2161 gataacacta acttccagtt ccgccggtaa agcgactgtt agtgcgacag tcagtgatgg
     2221 ggctgaggtt aaagcgactg aggtcacttt ttttgatgaa ctgaaaattg acaacaaggt
     2281 tgatattatt ggtaacaatg tcagaggcga gttgcctaat atttggctgc aatatggtca
     2341 gtttaaactg aaagcaagcg gtggtgatgg tacatattca tggtattcag aaaataccag
     2401 tatcgcgact gtcgatgcat cagggaaagt cactttgaat ggtaaaggca gtgtcgtaat
     2461 taaagccaca tctggtgata agcaaacagt aagttacact ataaaagcac cgtcgtatat
     2521 gataaaagtg gataagcaag cctattatgc tgatgctatg tccatttgca aaaatttatt
     2581 accatccaca cagacggtat tgtcagatat ttatgactca tggggggctg caaataaata
     2641 tagccattat agttctatga actcaataac tgcttggatt aaacagacat ctagtgagca
     2701 gcgttctgga gtatcaagca cttataacct aataacacaa aaccctcttc ctggggttaa
     2761 tgttaatact ccaaatgtct atgcggtttg tgtagaataa ttccataacc accccggcta
     2821 aaatatgtat tgttttagtc ggggcataat tatttcttct taagaaataa cctcttataa
     2881 tcaaatctac tactggtctt tttatctgct taataggtct ctttcaaaga gacacattca
     2941 cgttttctca tgtaggttga tccaaccacg ctgataccaa agctgaatca catcaagcaa
     3001 caactatgct cacaacatcc acacaataaa aaaccctccg aagaggggga agaggataag
     3061 aaattaatac gacagtggaa tatgtatata ttcgtctggg cgggagatgt tgataccatc
     3121 tttaggatca atcgcgtcta tgacatagcc tcctttcaga atgctaccaa tccccaggct
     3181 ggtgccatta tcaagagcaa ttgttggatt cttcatcgta ccgctgattc tgagtatttt
     3241 tccggggaaa atatctgctg aaatgtacgg ctgaatgttc tgaaagagta tcttggcgtt
     3301 actatttttg ttcattgcat ttataatggc aaggatctta ctctcattct gttgagaa
//
LOCUS       DAZKFZ010000314         3338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000314 DAZKFZ010000000
VERSION     DAZKFZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..692
                     /locus_tag="ACHOQW_004641"
     CDS             <1..692
                     /locus_tag="ACHOQW_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP9575556.1"
                     /translation="NVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAV
                     RRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIG
                     GKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTL
                     LFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAK
                     RIARLAEQSTD"
     gene            791..1804
                     /gene="ybjS"
                     /locus_tag="ACHOQW_004642"
     CDS             791..1804
                     /gene="ybjS"
                     /locus_tag="ACHOQW_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9575557.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1801..2631)
                     /gene="amiD"
                     /locus_tag="ACHOQW_004643"
     CDS             complement(1801..2631)
                     /gene="amiD"
                     /locus_tag="ACHOQW_004643"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP9575558.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2628..2951)
                     /gene="ybjQ"
                     /locus_tag="ACHOQW_004644"
     CDS             complement(2628..2951)
                     /gene="ybjQ"
                     /locus_tag="ACHOQW_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9575559.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3162..>3338)
                     /locus_tag="ACHOQW_004645"
     CDS             complement(3162..>3338)
                     /locus_tag="ACHOQW_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575560.1"
                     /translation="YEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGED
                     FRNHLQYKLTLPTH"
BASE COUNT          779 a          906 c          851 g          802 t
ORIGIN      
        1 tcaatgtgat tacttccgta ccgcccacca ccgccagggc gttgattcag gggctgaaac
       61 acgatctgct ggcggatgac accgcgctac gtgcgctcat cccacaacgg ctgatcgctt
      121 tcgatgacgc ggtacgtcgc accctgaaag aagaagaaaa gctggtcaac tccagcgact
      181 ggggatacga cactcaggcc tttgcccgct ggcgaccaga gtatggttat tttgccaaac
      241 aggcgggatt taccgttaaa acgtccgcca gccttgcggc tttatggcag gtggtgaacc
      301 aaatcggcgg taaagagcgt tatttctttg gcaatatttt gtggcagaca cgggcgttga
      361 tggaccgtgc gatcggtcat aaattagcga aaggccgtcc ggagcgcgaa tatttgcaaa
      421 ctggcgatgc ggtggatagc tggaaagtga ttgtcgttga accggaaaaa caacttacgt
      481 tgttatttgg catgaaagca ccggggctgg gacgactgtg ttttaccctg gaagataaag
      541 gcgactatcg tactatcgat gtccgcgctt tctggcatcc gcacggtatg ccggggctgt
      601 tttactggtt attgatgatc cccgcgcatc tgtttatttt tcgcggaatg gcaaaacgaa
      661 tcgccagact ggcagaacaa agcacagatt aatcgacaaa acctgcgtta ttctttcatg
      721 tttcccattg catcaagggg gaaatcacgg aagaatgcgc acgtcatttt ctcaacaaca
      781 gtggattgat atgaaggtac tggttaccgg cgccaccagc ggcttaggtc gaaacgcggt
      841 agagttttta tgccagaaag gcatcagcgt gcgagcgacc ggtcgcaacg aggcaatggg
      901 taaattgctg gaaaaaatgg gcgcagagtt tgttccggcg gatctgaccg agctggtctc
      961 atcacaagct aaagtgatgc tcgcgggcat tgatacgctg tggcactgct ccagctttac
     1021 ctctccctgg gggacacaac aggctttcga tctggctaac gttcgcgcca ctcgccgcct
     1081 aggtgaatgg gctgtcgcct ggggtgtacg taactttatt catatctctt ctccctccct
     1141 gtacttcgat tatcaccacc atcgcgatat taaagaagat tttcgccctc accgcttcgc
     1201 caacgagttt gcccgcagca aagcggccag cgaagaagtg atcaatatgc tttcgcaggc
     1261 gaatccacaa acgcgcttta ctattctgcg cccacaaagt ctgttcggac cgcacgataa
     1321 agtctttatc ccccgtcttg cgcatatgat gcaccactac ggcagcattc tgttaccgca
     1381 tggcggcagt gcgctggtgg atatgaccta ctatgaaaat gccgtgcacg ccatgtggct
     1441 ggcaagccag gaagcctgcg ataagctacc ttccgggcgt gtgtacaaca tcaccaacgg
     1501 cgagcatcgc acactgcgca gcatcgtgca gaagctgatc gacgagttga atattgactg
     1561 tcgtattcgt tccgtccctt acccgatgct ggatatgatc gcccgcagca tggagcgttt
     1621 aggccgcaag tcagcaaaag agccgccgct gacccactac ggcgtctcga agcttaattt
     1681 tgactttacg ctggatatta cgcgggcaca ggaagagtta ggttatcagc cggtcatcac
     1741 cctggatgaa ggtatcgaga aaactgccgc ctggctgcgc gaccacggaa aactgccgcg
     1801 ctaatcctgc ccgtatttct ccagcaacgc ttcggcaatc gcctgagttt ctgcatccgc
     1861 ttcgccgtta tataacgtcg ggcggaaatg catctggaac gccataatca cgcgccgctg
     1921 ctcgcgcggt gtcatatcag gtttaacgtc ataaccgtag cgcgccaaaa gctccagcaa
     1981 tgacgcggta tctaccggtg tgtgcggcgc gcgcccggca aggtaaaagt tcacccgctg
     2041 cgcatccggc caggcaccaa tcccctgctg cgccagttgc tgccagggaa ataatggccc
     2101 cggatcgtct ttgcgctgcg gggcgatatc cgcatgtgcc actacgtttt ccggcttgat
     2161 gtgataacgg gcaataatat ctttcgccag cggaataagc gcctgaatct gtgccggttc
     2221 aaacggggca aaatatttca cccccgccga tttttgccag ccacggtttt ccagctcaat
     2281 gccaatagag gtgtcgttaa ggcgcgttgc cccgcgccag gcgctaatcc ccgcatgcca
     2341 ggccagttct tgttccggca ccaattgcca gatgcgcggt ttaccgttgt atcgcggtgg
     2401 taccgcaggg accagataat gcgagctgac ctgcttatcg gtcagtgtcg ccagcgagct
     2461 atcaaaatca tctgcggtgt agtggatcac cagcacttta atgcgcggat acgccgcctg
     2521 cgcctggcgt cgggtatcaa gctgatatcc ctctttctcg acaatgcctt tttcgcctgc
     2581 gcaccctgcc agtaacagag cgacagcgat cagccagaag actcttctca tcgacgcgtt
     2641 ttcaccgccg taccgctgac gctaaccatc agcatactgc cgttttgccc gaccgtttcg
     2701 tagtcgatat caataccgac tacggcatcg gcccccagcg cccgcgcctg ggagccaagc
     2761 tcctcaaagg cgatctcccg cgctttacgt agctcttttt cgtaggcacc ggagcgtccg
     2821 ccaacgatat cgcgaatacc agcaaagaaa tcacggaaaa tattcgcgcc taaaatcgct
     2881 tcgccagtca ccacaccgca atattcaacg atggtctggc cttccagagt tggagttgtg
     2941 gaaaattgca tgatcgatct ccttgtttgt taagccttcg atctctaaag cattatcgga
     3001 ctgatatgcc atgttgggaa gaaggttttg atcagtttag gaaatcatct cattgtgtca
     3061 aaaacagcta cataagcctg tacaattatc tgtttcgtaa ccggatattg gtttcattat
     3121 ggagcaccgc ttacctgccc tattatgttg tgccggcaac attaatgagt gggaagagtc
     3181 aacttatatt gtaaatgatt cctaaagtct tccccatctt gcgcctgagg tatataccgc
     3241 tggattgctt tttggtccgc cgccgcaaaa caagaaagta ataagatctc aatgcttttg
     3301 tcattaatat aagcgaagat cattcttgca aattcgta
//
LOCUS       DAZKFZ010000315         3326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000315 DAZKFZ010000000
VERSION     DAZKFZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHOQW_004646"
     CDS             <1..384
                     /locus_tag="ACHOQW_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9575561.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
     gene            418..1134
                     /locus_tag="ACHOQW_004647"
     CDS             418..1134
                     /locus_tag="ACHOQW_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9575562.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            1131..1448
                     /locus_tag="ACHOQW_004648"
     CDS             1131..1448
                     /locus_tag="ACHOQW_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9575563.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            1445..1747
                     /locus_tag="ACHOQW_004649"
     CDS             1445..1747
                     /locus_tag="ACHOQW_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9575564.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            1737..2054
                     /locus_tag="ACHOQW_004650"
     CDS             1737..2054
                     /locus_tag="ACHOQW_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFP9575565.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            2008..2325
                     /locus_tag="ACHOQW_004651"
     CDS             2008..2325
                     /locus_tag="ACHOQW_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575566.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            2312..2749
                     /locus_tag="ACHOQW_004652"
     CDS             2312..2749
                     /locus_tag="ACHOQW_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9575567.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            2751..2942
                     /locus_tag="ACHOQW_004653"
     CDS             2751..2942
                     /locus_tag="ACHOQW_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575568.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            2945..>3326
                     /locus_tag="ACHOQW_004654"
     CDS             2945..>3326
                     /locus_tag="ACHOQW_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575569.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPD"
BASE COUNT          920 a          693 c          992 g          721 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgtgacaga
      181 gggattgagc ggcagatgac ggaaggtgag ttaaaacgac ttgcagaacg gcaactgacg
      241 aaatgggcaa agcaagttgg taacgggatg agtattcccc cgatccggag acaactcgca
      301 tcgccgaagt gcccgcaagg gccaacgcca attgaattac tgaaacagga atacgagcgc
      361 cggaaggcgg ctggttttgt ttgaatctga gaaacgattt tgtcggagga aattttaatg
      421 gaaaccgtat ctgacgcact gaaagcactg aaaagagcct cttcacatgt ggtggcagct
      481 cgccttggaa ttagtcgtga agaggctgtc aacgaactgt ggaaactgaa acgccgtgga
      541 gaagcggata acaagggggc gatatggtgg ctgactcagg ctggtgaaag tgaaccggtg
      601 tcaccggtac cgaaagtgac ggcgcaaatg ctgacagagg cgattgaaca acatggccca
      661 caaacggcgg atgaactggc attgatgttc ggaattacct cccgccgggc gaattcatcg
      721 ctggcaatgg caatcagcaa agggtgtctg attcgcgtga atcaggatgg taaatttcgt
      781 tactgcatac cgggcgttga tttaccggca gagccgaaag cagcatccgt agctgaaacg
      841 gagggtaaag cccttcctca gccagctggt gttgcgttac cagtccagga aacggctgca
      901 caggaagaaa ttaaaacaga agcggtggag gacattgtga agttgcagca atcgttcact
      961 gaagcgaaag cagatgacct gattctacca tcgctgcatg tggctaaccg cgagctgcgc
     1021 cgggcgaaaa gtaatgttca gaagtgggag cgagtctgtg ctgcgctacg ggaactgaac
     1081 aaacacaggg atattctccg ggatattacc gccaccagag agcagcagcg gtgagtggct
     1141 ggaagaagtg gcgctgggct gaaatcctga tactccggca gtgtgcggga acgatgagag
     1201 tcgaaagcat cggttatctg attggccgta gtgagtcagc cgtcaggacg aaagcgcggg
     1261 aactgggtat cagcatgatg ttacggggtg attatcacca gtcagccaaa tgttcacagc
     1321 gtgatattga gctggcgtgg caactgcatc agcgtggtgt accccgacgg gaaattgccg
     1381 aaaagtttgg gatgaagttg ggcgcagtga ataactacgt ttatttcgac aggagggttc
     1441 aggagtgagg gtgagggttt atatcgccgg tccgatgacg ggatatgaaa attttaaccg
     1501 cgaggcattt cacaaaacgg aagaggtgct gaaacgggaa gggcataccg ttttaaaccc
     1561 ggcagtactt ccggacgggc tgactcagcc acactacatg gatatttgca tggcaatgct
     1621 ccgttgcgtg gatgcggttt acatgctgaa aggctggcag cagtcggcag gtgcaggggc
     1681 tgagctggca ctggaggaga aaccgggcca tgcggtgatt tttcaggagg tgggcagtga
     1741 atattgaccc ggcgataacg attgatatgg ccctgaacgc cggcctggca cttcttggtt
     1801 atttctacat tatgttctgc agcggacgat ggctgtcact gttgttcatg aaaaaatgga
     1861 ataaacgccg taagcaggag caacgccaga aggcaatgga tgcatttttc gaagccttcg
     1921 gaattgacgg catggaacca ggggatccag ctcgcgcaat cagcagaggg ggtgtagtaa
     1981 tccttgtata tcggagtgaa gagaaaaatg acgatcacaa aacaacgtgt agaagaaatc
     2041 atatcccgca ttgaaatgta tggacatggt gcagggtata tcgcagacga ggttaatgat
     2101 ctggctatac tggcgctgaa tttatcaaat atcgcgaacc tgaagcgata cgagcttgat
     2161 atgggaggtt gcgactcgtg cggtcaggat tgtggcgctg atatgactga agatcctgat
     2221 ggtgattatg tcctgtttga tgacgtggtt aagttgtttg aatttgatac aaccactcaa
     2281 aagttagaaa tcccggcaaa ggaggcagcc agtgggcaag attgactatc aggcactgcg
     2341 tgaggcagca caaaactatc aatcgacgct ggcgtggtat caggctaccc cggacagccc
     2401 aaatgctgaa cgggattgtg atgcggctct tgctgcgttt aagcgtcaca tccgtcatcg
     2461 ggaagcggat attatcgctg atttgctgga tggactggaa gaagcaaaat cacaactcaa
     2521 cgagcagcgt gagtattacg aaggcgttat ctctgatggg agcaagcgta ttgctgaact
     2581 ggaagcgcgg gaagttcaat taccgactcg ctacgacctt cgatatggac acccgataaa
     2641 tgcagatgag cgacatgtca tgatacctaa agaaaatggc agttggcttt acctgattga
     2701 cctagaacac gcattacgcg tcgctggcat tcgcatcaaa ggagagtgag atgaacggac
     2761 aaatatcaat tgttcgacca ggagcatgtg acgatcgcga aatacgaatg attattcgtc
     2821 tggcgatggg gaaaacaata actgctctca ttactccaga aaatctcgca ttagcattaa
     2881 caggaaagtc agacatgcca gtagagctaa agctgcgaaa tgttgagatt aaggtgaaat
     2941 agctatgacc actattacca aagatcgact gctgacaatc cagcattggc gcgaaacata
     3001 cggaccgggt agcaacgttg tgcttccagc agaagaagcg gaagagctgg cacggattgc
     3061 gctggcatca ctggcagcag tatcggatga acgagcagcc tatgaattat ttatggagaa
     3121 gcgtttcgga gaatctgtag atcgccgcag agcaaaaaat ggcgatagag attacatggt
     3181 atgggatatg gcgcttggct ggattatctg gtgtcaccgc gccgccatgc ttcaggctgg
     3241 aaactttcgg gaaaataagg gttcgtcaac caataatttt cgggaaatct cggaaacgtc
     3301 aaccaactat ccggtaactc cggatg
//
LOCUS       DAZKFZ010000316         3303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000316 DAZKFZ010000000
VERSION     DAZKFZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.318"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            86..1741
                     /gene="aslA"
                     /locus_tag="ACHOQW_004655"
     CDS             86..1741
                     /gene="aslA"
                     /locus_tag="ACHOQW_004655"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFP9575570.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1909..3144)
                     /locus_tag="ACHOQW_004656"
     CDS             complement(1909..3144)
                     /locus_tag="ACHOQW_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9575571.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
BASE COUNT          826 a          883 c          832 g          762 t
ORIGIN      
        1 taatcatcct gtcagggaga gggatctcaa ttatcaatgc ttaattacgt catcattttg
       61 ttgacatgaa accggagcat tttgcatgga attttcgttt tcacccaaac ttcttgttgt
      121 tgctgtcgcc gccgctcttc ctctcatggc cagcgcagca gataccccgt caactgccac
      181 cgcgcgcaaa ggctttgccg gatacgatca cccaaaccag tatctggtta aaccggcgac
      241 cactattgcc gacaacatga tgccagtaat gcagcatccg gcgcaggata aagaacccca
      301 gcagaagctg gcagaacttg agaaaaaaac cggtaagaaa ccgaatgtgg ttgttttctt
      361 gctggacgat gtgggctgga tggacgtcgg ttttaacggt ggcggcgtgg cggtgggtaa
      421 ccctacacca gatatcgacg ccgttgccag ccaggggctg attttaactt cggcgtattc
      481 tcaaccaagc tcttccccaa cccgcgccac cattctcacc ggacaatact ccatccacca
      541 cggcattctg atgccgccaa tgtacgggca accgggcggg ctgcaagggt taaccacgct
      601 gccgcagttg ctgcacgatc agggctacgt cactcaggcc atcgggaaat ggcatatggg
      661 ggaaaacaaa gagtcgcagc cgcagaacgt tggctttgat gatttccgtg gctttaactc
      721 ggtgtctgat atgtacaccg aatggcgcga cgttcacgtc aatccggaag tggccctgag
      781 tccggaccgt tctgaataca tcaagcaatt accatttagc aaagatgacg ttcatgcggt
      841 gcgcggcggc gaacaacagg ccattgccga cattacgccg aaatatatgg aagatctgga
      901 tcaacgctgg atggaatatg gcgttaagtt cctcgacaag atggcgaaga gtgataagcc
      961 tttcttcctc tattacggca ctcgcggctg tcacttcgat aactacccga acgccaaata
     1021 tgcgggtagc tctccggcac gcacctcgta tggcgactgc atggtggaaa tgaacgatgt
     1081 gttcgctaat ctgtataaag cactggagaa aaacggtcag cttgataaca cgctgattgt
     1141 ctttacctcc gataacggac cggaagccga agtaccgccg cacggacgca caccgttccg
     1201 tggtgcgaaa ggatctacct gggaaggcgg cgttcgcgta ccgactttcg tttactggaa
     1261 aggcatgatc caaccgcgta aatctgacgg tattgttgat ctggcagatc tcttcccaac
     1321 agcactggat ctggcggggc atcctggtgc gaaagtggcg aatttagtgc cgaaaaccac
     1381 ctttatcgat ggtgtggacc agacatcttt cttcctggga acaaatggtc agtctaaccg
     1441 taaggccgag cactacttcc tcaacggtaa actcgctgct gtgcgtatgg atgaattcaa
     1501 gtatcacgtc ctgattcagc aaccttacgc ttatacccag agcggatatc agggtggatt
     1561 caccggcaca gtaatgcaaa cagccggatc gtcggtgttt aacctctaca ccgatccgca
     1621 ggagagcgac tctatcggcg tgcgccatat tccgatgggt gtaccgctac agaccgaaat
     1681 gcacgcgtat atggagatcc tgaaaaagta tccaccacgc gcgcagatta aatctgatta
     1741 agccggcgct atcgctgaga tctgcctttg ccggatgcga tgctgacgca tcttatccag
     1801 cctacagaac gctgcaattt attgaatttg cacgatcatg taggccggat aaggcgttta
     1861 cgccgcatcc ggcaatcaac cgcaggcgac cacaaaatgg ccgctttttt acttactcac
     1921 caccagcaaa tgcgcctgca taatgtcgct ggccgggcga ccgtgcgcca gcaaatcagc
     1981 cattgcttta agatatggcg gtagatggcg gaaataacgc tgatacccgg cacacaaata
     2041 attcagtccc ggtttgccgc tggcatcgag catgaagcgg tgtttcgggc agcctcccca
     2101 acatgctttt aacacgttac aactgcgaca ctgcgccggt aattgcttaa atttatcttc
     2161 accaaacacc tgctgttgcg gggaatcgat catttctgca attgtttgct ggtgcatatt
     2221 ccccagtcga tattgcggat aaacatagtg atcgcaggcg taaacgtcgc cattgtgctc
     2281 aacaatcacc gagcgcccac aggttggctg atgatggcaa accgcacccg gcgcaccgac
     2341 aaaattggca aacgcccatt cgatattcat cacgaaaatc ttgccgacgt cgcgtttaat
     2401 ccagtggtcg aatatcgcca ccagaaattc accaaattca tcggggcgca cagaccattc
     2461 cgtcagttcc ccctgaatat caccaggcgc atgtagtttc agtccttcgc gggcagtcgt
     2521 ttcatcagcc aggcgctcga ccaccggaat aaactggatg aattcgactc ccgcatcgca
     2581 caaaaaatca taaacctgta acggttgctg cgcgctggtg cggttgacgc agaccagcac
     2641 gttatagtcg acatgatgtt tttgcaggag cgtcagggca cgcatcacca gtttatgcgt
     2701 gggtctgccg ccttttgtca cgcgatattg attgtggatc tcagccggac catccagcga
     2761 taacccaaca agaaaatgat tttctgccag aaatgcacac cattcatcat cgagcagtac
     2821 gccgttagtc tggaagctgt tacttatctt cctgccagca ccatatttcg cctgtagcgc
     2881 cacggcacgg cggtaaaact ccagcccgag tagcgttggt tcgccgccct gccaggtaaa
     2941 agccacttcg ttttgcggtt cgctggcagc gatatagtga cggacatacg cttccagcgt
     3001 gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg gattgttttt cgagataaaa
     3061 acagtagtca cagttcagat tacaatcgga accgctcggt ttcgccatca catgaaaagc
     3121 acgcgttgga acctgttgca gcatggtcgc tccttgcctg aagtcatcct tcaatacgtt
     3181 aagggcgaaa tcataccgtg gggcaatgag ttattacggg cgtttattgc gaagggaggg
     3241 tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat tggtcggcga
     3301 gag
//
LOCUS       DAZKFZ010000317         3247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000317 DAZKFZ010000000
VERSION     DAZKFZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..281)
                     /gene="ybgE"
                     /locus_tag="ACHOQW_004657"
     CDS             complement(<1..281)
                     /gene="ybgE"
                     /locus_tag="ACHOQW_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP9575572.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLI"
     gene            complement(281..394)
                     /gene="cydX"
                     /locus_tag="ACHOQW_004658"
     CDS             complement(281..394)
                     /gene="cydX"
                     /locus_tag="ACHOQW_004658"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP9575573.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(409..1548)
                     /gene="cydB"
                     /locus_tag="ACHOQW_004659"
     CDS             complement(409..1548)
                     /gene="cydB"
                     /locus_tag="ACHOQW_004659"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9575574.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1564..3132)
                     /gene="cydA"
                     /locus_tag="ACHOQW_004660"
     CDS             complement(1564..3132)
                     /gene="cydA"
                     /locus_tag="ACHOQW_004660"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP9575575.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT          894 a          908 c          807 g          638 t
ORIGIN      
        1 aaaatcagcc cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaat cccttgccaa
       61 agaaccttct gcggacgaaa gcccacgccg tgaatcacac cagcacagac ggcccacatc
      121 agcaataaac catgccagat ttccagttca ctggtcttcg cggcgaaacg agatgggtcc
      181 caaaacatac atcctgctaa cagaagcgcc atcacgaagg aaagcgcccg cagggggcgc
      241 ttgtccatta ccgcatacaa ggttgcgata atcttactca tcagatgtct tcttgaccgg
      301 ctttgcctga ttcgacgtgc tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa
      361 gaagcgttcc cagaatccat gcgaaatacc acatttttag ctccttactt agtacagaga
      421 gtgggtgtta cgttcaatat cttctttggt gatacgaccg aacattttcc agtaacacca
      481 ggcggtgtag agcagaatga tcggtaccag aaccaccgca acccaggtca tgacgttaag
      541 tgtcagctgg ctggaagttg catcccacat tgtcagactt gcgttcatca tggtgctgga
      601 cggcatcaca aacgggaaca ttgcgatacc ggcagtcagg atgatgcagg ccagcgtcag
      661 ggaggagaac acaaacgccc acgcggcttt atccatacgt gcagtcagga tggtcagcag
      721 cggcagaacc acacccagtg ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac
      781 cagccatgcg ccagcttcac gaaccacttc tttattcagc gggttagagg ctgcgtaatg
      841 gtccattgtc gatttcacga cataaccatc gataccgtac atcacccata cgccagccag
      901 tgcgaaacag accagtgtca ccagtgccgc cacctgagcc gttgcacggg tacgcaggtg
      961 cagttcgccc acggtacgca tttgcagata ggttgcgccc tgagtaatga tcatccctac
     1021 gctcaccacg cctgccagca ggccgaacgg gttaagcagc tggaagaagt taccggtgta
     1081 gtacagacgc agatattcat caacgttgaa cggtacgccc tgcaacaggt taccgaacgc
     1141 tacaccaatc accaacggcg gaacgaagct accaatgaag atgccccagt cccacatgtt
     1201 acgccagcgg gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa
     1261 agacgccagc acgaggatca tcgccacata gaagccggag aacgcagcgg catagaccat
     1321 cggccaggca gcaaacagtg cgccgcctgc ggtgatcagc caaacctggt taccgtccca
     1381 gtgtggtgcg atggagttaa tcataattcg acgctcggtg tcgttacgac cgaggaaacg
     1441 ggtgagcatg cccaccccca tgtcgaaacc atcagtgact gcaaaaccaa tcagcagaac
     1501 gccaaccagc agccaccaga taaaacgcaa tacttcataa tcgatcattt gacgactcct
     1561 gtcttagcgt gccggctgag tagtcgtgga agactgctca aagtgatagc gaccggtttt
     1621 caggctgctt gggccgaggc gtgcaaactt gaacattaag aacaattctg ccaccaggaa
     1681 cagggtatac aggccgcaaa tcagcaccat tgagaagatg agatcgcctg cggtcagtga
     1741 cgagttcgcc acagctgtcg gcagcacttc accgatagcc cacggttggc ggccatattc
     1801 agccacgaac cagcccgctt ctacagcaat ccacggcagc ggaataccgt acagcgcggc
     1861 gcgcaggagc cattttttct cgccaatgcg gttgcggatg acactccaga aagagagcgc
     1921 gatgattgcc agcagcagga agccacacgc caccatgata cggaacgcga agtacagcgg
     1981 cgctacacgc ggaatggagt ctttggttgc ctgttgaatc tgcgcttcag tcgcatcagc
     2041 cacgtttggc gtatagcgtt tcagcagcag accgtaaccg aggtccttct tcatgctatt
     2101 gaactggtca cgaaccgcct ggtcggtaga accagaacgc agttgttcga gcagagagta
     2161 cgccttcatc ccgttacgaa tgcgttcttc atgctgcacc atcagttctt tcaggccgat
     2221 aactggagta tccacggaac gcgttgcaat tatgcccagc gcgtaaggga tctggatcgc
     2281 aaatttgttc gtctcctctt cctgatcagg aatgccgaac agagtaaagg cagcaggcgc
     2341 aggttgcgtt tcccactcag cttcaatagc agccagtttg gttttctgca cgtcgcccat
     2401 ttcgtagccg gattcatcac ccagaacaat aacagacaga acggcagcca taccgaagct
     2461 ggcagcgata gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca gcatatacca
     2521 tgcgctgata ccgaggatga acatcgcgcc agtcacataa ccagacgcta cagtgtgaac
     2581 gaatttcacc tgagcaaccg ggttaagcac cagctcggag aagctcacca tctccatacg
     2641 catagtttca aagttgaaat cggacgcgat tgggttttgc atccagccgt tcgcaaccag
     2701 aatccacagt gcggacaggt ttgaaccgag cgccaccagc caggtgacac acatatgctg
     2761 aactttaccc agacgatccc aaccgaagaa gaacagacct acaaaggtgg attcgaggaa
     2821 gaaggccatc agaccttcga ttgccagcgg cgcaccgaag atatccccta catagtggga
     2881 atagtaagac cagttagtcc cgaactggaa ctccatggtc agaccggtag ccacacccag
     2941 agcgaagttg ataccaaaca acttgcccca gaacttggtc atatctttat aaatctgttt
     3001 gccggaaagg acgtagaccg tttccataat ggccagcagg aacgccatac cgagcgtcag
     3061 tggcacaaaa aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg acagttcgac
     3121 tatatctaac atcatgactc cttgctcatc gcatgaagac tccgagagtg aaccccgtta
     3181 gaaagggtca cacgcatgcc ccaatacaaa attatttgct cgcctctatc gttgccattt
     3241 catcaaa
//
LOCUS       DAZKFZ010000318         3227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000318 DAZKFZ010000000
VERSION     DAZKFZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..109
                     /locus_tag="ACHOQW_004661"
     CDS             <1..109
                     /locus_tag="ACHOQW_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575576.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
     gene            100..588
                     /locus_tag="ACHOQW_004662"
     CDS             100..588
                     /locus_tag="ACHOQW_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9575577.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            902..977
                     /locus_tag="ACHOQW_004663"
     tRNA            902..977
                     /locus_tag="ACHOQW_004663"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:935..937,aa:Ile,seq:cat)
     gene            979..1055
                     /locus_tag="ACHOQW_004664"
     tRNA            979..1055
                     /locus_tag="ACHOQW_004664"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1013..1015,aa:Arg,seq:tcg)
     gene            1069..1145
                     /locus_tag="ACHOQW_004665"
     tRNA            1069..1145
                     /locus_tag="ACHOQW_004665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1103..1105,aa:Arg,seq:tct)
     gene            1238..2197
                     /locus_tag="ACHOQW_004666"
     CDS             1238..2197
                     /locus_tag="ACHOQW_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFP9575578.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            2209..2478
                     /locus_tag="ACHOQW_004667"
     CDS             2209..2478
                     /locus_tag="ACHOQW_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFP9575579.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(2775..3098)
                     /locus_tag="ACHOQW_004668"
     CDS             complement(2775..3098)
                     /locus_tag="ACHOQW_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP9575580.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          858 a          662 c          785 g          922 t
ORIGIN      
        1 agcagtgaaa ccactcaaat ttgccaacca gatgtatatc gataccggcg cagtgttctg
       61 cggaaatctc acattgattc aggtacaggg agaaggcgcg tgggcataag agaactaaac
      121 ctcaccaaag aacagcatga gtggctgaat ggctggcttg aactgtgggg cgcatgggtt
      181 tattcaggtc gtctggaaaa gcgcatgagc agcgtaatag ctaaattcat ggagagcgtg
      241 gagccgggaa gagttatgac aaggccaatg tgtaatgatg atgatggaat gttgatttct
      301 caggtcgtcg attccgtcat gtacattgac aagaaagcct ttggcatcct cctcagctac
      361 tacgcccacg gctcttccaa gcacgccatt gcatcttact atcatagcgt cgcaagacct
      421 cgcaagatgt tatgccgggg cggcgggcgc attcaaaaac catcgctagc aacctgccga
      481 cgggaagttg acgaaatcct caatgcctcg ttgtttatga tttacccgat tctggatagt
      541 gcgtttaaaa accggaaacg tgtagagaaa attaagcatg tagcatagaa cgtgttgaca
      601 tcgttgagca aatgagcaac actattcgca taagctgccg ttagtgactc ttaagttgca
      661 acggtggctt tttttatttg ggtcaatcgt ataaaggtca ttagagcctg taactgtttt
      721 tcgtccataa attaaccttc atttgatgct ggattgaaca tatcaaaatc aggcaattac
      781 acaaatctat gtacaggctc tttgtgcctg acgttaaaag attttttaca agaataattt
      841 tgaatcagtg aatttgtgaa ctcttgcaac attgatttcg taacgttatt atcctacgct
      901 cggcccttta gctcagtggt gagagcgagc gactcataat cgccaggtcg ctggttcaaa
      961 tccagcaagg gccaccaacc gccattagct catcgggata gagcgtcagc cttcgaagct
     1021 ggttgcgcga ggttcgagtc cccggtggtg gtccattatc ggtattctgc gttgttagct
     1081 cagcaggaca gagcaattgc cttctaagca atcggtcact ggttcgaatc cagtacaacg
     1141 cgccacactt attttccctg gctcgctttt gcgggccttt tttatatctg cgccgggtct
     1201 ggtgctgatt acttcagcca aaaggaacac cttgtatatg aagtgtatat tatttaaatg
     1261 ggtactgtgc ctgttactgg gcttttcttc ggtatcctat tcccgggaat ttacgataga
     1321 cttttcgact caacaaagtt atgtatcttc gttaaatagt atacggacag agatatcgac
     1381 ccctcttgaa catatatctc aggggaccac atcggtgtct gttattaacc acaccccacc
     1441 gggcagttat tttgctgtgg atatacgagg gcttgatgtc tatcaggcgc gttttgacca
     1501 tcttcgtctg attattgagc aaaataattt atatgtggcc gggttcgtta atacggcaac
     1561 aaatactttc taccgttttt cagattttac acatatatca gtgcccggtg tgacaacggt
     1621 ttccatgaca acggacagca gttataccac tctgcaacgt gtcgcagcgc tggaacgttc
     1681 cggaatgcaa atcagtcgtc actcactggt ttcatcatat ctggcgttaa tggagttcag
     1741 tggtaataca atgaccagag atgcatccag agcagttctg cgttttgtca ctgtcacagc
     1801 agaagcctta cgcttcaggc agatacagag agaatttcgt caggcactgt ctgaaactgc
     1861 tcctgtgtat acgatgacgc cgggagacgt ggacctcact ctgaactggg ggcgaatcag
     1921 caatgtgctt ccggagtatc ggggagagga tggtgtcaga gtggggagaa tatcctttaa
     1981 taatatatcg gcgatactgg gcactgtggc cgttatactg aattgtcatc atcagggggc
     2041 gcgttctgtt cgcgccgtga atgaagatag tcaaccagaa tgtcagataa ctggcgacag
     2101 gcccgttata aaaataaaca atacattatg ggaaagtaat acagctgcag cgtttctgaa
     2161 cagaaagtca cagtttttat atacaacggg taaataaagg agttaagtat gaagaagatg
     2221 tttatggcgg ttttatttgc attagtttct gttaatgcaa tggcggcgga ttgcgctaaa
     2281 ggtaaaattg agttttccaa gtataatgag aatgatacat tcacagtaaa agtggccgga
     2341 aaagagtact ggaccagtcg ctggaatctg caaccgttac tgcaaagtgc tcagttgaca
     2401 ggaatgactg tcacaatcaa atccagtacc tgtgaatcag gctccggatt tgctgaagtg
     2461 cagtttaata atgactgagg cataacctga ttcgtggtat gtgggtaaca agtgtaatct
     2521 gtgtcacaat tcagtcagtt gacagttgcc tgtcagactg agcattcgtt aaaaaaattc
     2581 gcatggtgaa tccccctgtg cggaggggcg actggtgtat aaggtgtcat ctctgacgac
     2641 aacctggagc gagatacgcg ggttcaggga caccggactg aactcaccgg gaggcacccg
     2701 gcaccatgta catgatgata cagatacgca gtgtcagccc ctctccggag gggcttttta
     2761 tctgaatgat tctgttattt tcgcccgtat gggcatattt ccagaatgca gcgatgatta
     2821 aaacacttat ctgtgatatt tcctgtgttt gcagggcact cctggctgtt tttaattaaa
     2881 ttccagacgt ttttattaaa tggggccacg ttgtaaatgg tgacggggag ctctttatta
     2941 ttgagtatta cgcctgtccg ggttggtgta aaaatacagc ccggagggag gaaggtatct
     3001 gactgatacc ataatattaa ttttatgtta caccatatgg ctgaaaatga tatgccacat
     3061 gctggctgga ttaccgtgtc aatcactatc cagttcattt gctttcctta ttttcttaag
     3121 gttttaaatg atgccataaa ttataggtga tgttttgaaa gcaatctgtc aggctgagtt
     3181 gtttttctgg tgtcgtttat tctttgttta aaataagagg gcatttt
//
LOCUS       DAZKFZ010000319         3214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000319 DAZKFZ010000000
VERSION     DAZKFZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..141)
                     /locus_tag="ACHOQW_004669"
     CDS             complement(<1..141)
                     /locus_tag="ACHOQW_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575581.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQR"
     gene            complement(125..352)
                     /locus_tag="ACHOQW_004670"
     CDS             complement(125..352)
                     /locus_tag="ACHOQW_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9575582.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            429..836
                     /locus_tag="ACHOQW_004671"
     CDS             429..836
                     /locus_tag="ACHOQW_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575583.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1100..1399
                     /locus_tag="ACHOQW_004672"
     CDS             1100..1399
                     /locus_tag="ACHOQW_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575584.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            1472..1690
                     /gene="ydfC"
                     /locus_tag="ACHOQW_004673"
     CDS             1472..1690
                     /gene="ydfC"
                     /locus_tag="ACHOQW_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9575585.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            1713..2120
                     /locus_tag="ACHOQW_004674"
     CDS             1713..2120
                     /locus_tag="ACHOQW_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575586.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(2098..2331)
                     /locus_tag="ACHOQW_004675"
     CDS             complement(2098..2331)
                     /locus_tag="ACHOQW_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575587.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            2890..3078
                     /gene="dicB"
                     /locus_tag="ACHOQW_004676"
     CDS             2890..3078
                     /gene="dicB"
                     /locus_tag="ACHOQW_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9575588.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            3075..>3214
                     /locus_tag="ACHOQW_004677"
     CDS             3075..>3214
                     /locus_tag="ACHOQW_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9575589.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYP"
BASE COUNT          924 a          726 c          728 g          836 t
ORIGIN      
        1 gcgttgaaca tcgatgttcg ggcgttcttt cagagcccag aatgcttccg tgattaatat
       61 cgtcgcctgc tcctgtgtca ttcctggtcg gcatacccag gtatccagag cctcacaaac
      121 ctgttcagtg gtgattttca ttgttcaacc gccccgcccg ctttgcctta cgatattcgt
      181 cataaacttt ggggtcgtac tgaagttccc cgccggatgc ctcttgcagg cgcatcgcgc
      241 gaccttcagg aaccagttcc ccccatgcag caacgcttgc cagcctaact cctgcgacat
      301 tggcaagctt tgttttgctg ccaaaaaacg ctatagcatc aattttcaac atatcgaaac
      361 ccttagattt tcctaaggaa actagatcgt agagaaacct aagtcaagaa aaattagaat
      421 tacctaatat gaaaaacgaa accttcggtg ctcgcctctt atacaggcga aaaaaattaa
      481 aactgtctca ggccgcatta ggtaagctgg tcaaagtggc tcacgtaaca atttctcaat
      541 gggaaagaga tgaaacacag ccagcgggga agagattatt cgcactgagc caggcgcttc
      601 agtgctcgcc gacttggctt ctttttggag atgaagataa gcaaccaggc gaaccgatcc
      661 cagataatca gccagtcaat ctgacagaag atcaaaaaga gttgcttcaa ctgttcgacg
      721 cactgcctga gtcagagcaa aaggctctgt tgtcagagat gcgtgctcga gttgagaatt
      781 tcaacaaact ttttgaagaa ctactcaaag ctcgcaaaag aagcgcaaat aaataacccc
      841 cctttttttc gcaactctct gtaataaaaa gcacaaactt tcaaatactt gtgtttttta
      901 catcaagaag cttaggtttt tctacacaaa aagcttgacc ataactctta ggctattcta
      961 aattctactc atcaagacac cgcacggtgt tctcagcaaa cagttccgct acccggcgtt
     1021 aaggggaagc agaggatttc tcagtgggcg agtcaaacat cagaatggaa ggcgtccagg
     1081 gatcagcaaa gaaacagcga tggcgcttta tattgatatc agcgccattg ccggacaggt
     1141 aagagttatc agagcggtaa ctaagcggta tgcgccttta cttcagaaag tctctggtga
     1201 gtgcaccgaa gatattgtca acgatttcgt catcgaactg cgaggactca tcttcagtta
     1261 caaggtgacc acaatttttg cagatggctc ccgcgaaact gtcagagccc tgcggcttaa
     1321 aggatgtgtc aaagacttag ccaccacatt ctgggcaaga aaacttgatt gtattcataa
     1381 ccaatttcct ctcgagtaac agacccctca gaggatacca cctcgcctga cgtggttaaa
     1441 aagcaggcaa cgctaaccac aaggagccga catgcagaaa cgagaccccg tcatcatcgc
     1501 gccagactat accgatgatg aactttatga gtggatgcac cagaaaatta aggcggtgca
     1561 ggacctgaaa tgggccaatg aagccagggc taagcaggct gaaaatctgt ccgctctgga
     1621 gcaggatatc accaatctgg aaaaagcagc ggcattaagc attgccagaa tgattacata
     1681 cccacgttaa tagctaacca acgaggctaa taatggaatt taaagattta ccaacgccat
     1741 tacaggaaat ggcatcgaat atagttcgtt cacaactggc tactcttgac cttagtaccg
     1801 cagaaaaaga aaccatcgat aatatggttc gtaatgtgcg caatgctttt tctgggctat
     1861 atggttctga taatcaaaag caggaaagcg atgttaataa acgggtaatt tctgtttgcg
     1921 tgaatggcca tgttctttca tcaatcaaaa cagaaacggc gacagtcttc gattgccttt
     1981 gcattgtaca gagccttgtt gatgccctgt ttcgttcagt gaatttagaa aatgatgcaa
     2041 atctgcgagg gcgcataata gcacatccat atgcacatac tttaggctct gtggatatca
     2101 aagatcccac aaatctttaa tgaaatagtt aacgcgaatt gtacttgctc tttcggttgc
     2161 tttcagaata cgcgttgaaa ctgctggcgg taatttggta ttccatttat taaaatcatg
     2221 cccgggaaag tactcttcga aaatactttt aactgcagac tcgcctattg aaatgctgct
     2281 taccatgcga ttttgataaa ggcatttagc aataagagtt gattttaaca ttcaccctcc
     2341 tgagggttgg taattaagga gttctccacg ggtgaagtgg agtgcgtgcg ccggacacgg
     2401 gtgaacatcc ggcactgaca gtttactgaa aggatatttc tctgaaaagt cagagcataa
     2461 cgcgaaagcg cacggcgagg ttgctggttc atagatagcc tgtcgttaaa ttttcgtcga
     2521 ccgtgcgctt ccggttgtgg cactccgcga aatggcgcag cggtaagtat ggcggggtta
     2581 ttccttcccc gttgaggaca ccgggttgtc aggttgacca tacgcttaag tgacaacccc
     2641 gctgcaacgc cctctgttat caattttctg gtgacgtttg gcggcatctg tttgcccatg
     2701 aactgatgtc cgcccttttt aaagtgaatt ttgtgatgca gtgaatgcgg ctaagcgcac
     2761 gcggaacagt taaagctaaa aacagtgtta tgggtggatt ctctgtatcc ggcgttaatt
     2821 gttaactggt taacgtcacc tggaggcacc aggcaccgca tcacaaaatt cattgttgag
     2881 gacgcgataa tggaaacgtt attaccaaac gttaatacgt ctgaaggttg ttttgaaatt
     2941 ggtgtcacta tcagtaaccc tgtatttact gaagatgcca ttaacaagag aaaacacgaa
     3001 cgggagttat taaataaaat atgcattctt tcaatgctgg cccgtttacg tccgatacaa
     3061 aaaggatgct ggcaatgaat acagcatttg cacttgttct gacagttttt cttgtttccg
     3121 gagagccagt tgatattgca gtcagtgttc acaggacaat gcaggagtgt gtgactgcag
     3181 caaccgaaca gaaaattccc ggtaactgtt accc
//
LOCUS       DAZKFZ010000320         3208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000320 DAZKFZ010000000
VERSION     DAZKFZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..368
                     /locus_tag="ACHOQW_004678"
     CDS             <1..368
                     /locus_tag="ACHOQW_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9575590.1"
                     /translation="KDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPI
                     NYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKN
                     CFAEQEILSEFPQPVSLAG"
     gene            complement(411..1562)
                     /locus_tag="ACHOQW_004679"
     CDS             complement(411..1562)
                     /locus_tag="ACHOQW_004679"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP9575591.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1576..3171)
                     /locus_tag="ACHOQW_004680"
     CDS             complement(1576..3171)
                     /locus_tag="ACHOQW_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP9575592.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGSNVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT          805 a          815 c          722 g          866 t
ORIGIN      
        1 taaaagatat tcttttctat ctgaataata attatcgcga aaaaatcact ttagaacagt
       61 tatcgaaaaa atttcgtgcc agcgtcagtt atatttgcca tgaatttacc aaagagtatc
      121 gtatttcccc tattaactat gttattcaac ggcgtatgac ggaagcaaaa tggtcactca
      181 ctaatactga attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc
      241 actttgccaa actgtttttg cgccatgtcg gctgttcgcc cagcgattac cgcaggcaat
      301 ttaaaaactg ttttgcggaa caagaaatcc tatctgaatt tcctcaaccg gtaagtcttg
      361 ccggataaat ctggcggcgc agtttgttaa gaagctgcgc cgcctgcaga ttatttgttc
      421 tgatagtctt tcaggatctg ccgacctgct acgtaaatca taatttcgtc tgtaccgccg
      481 ccaatacgtt cacaacggac atcacgccag aagcgggaga cgcgcgcctc atcggtatag
      541 cccagaccgc ccatgatttg aatcgcatca tcaatgactt ccattgcggt acgtgcacag
      601 tacagttttg ccagcgccgc gctggtgcgc agtgattgat gctgatcggc ttgccatgcc
      661 actttcagca ccatgttgcg catgttgtca atcttaatcg ccatcagcgc cagtttttcc
      721 tggatcatct ggttatgacc aatgggttta ccaaaagcga tacgctggtt ggcatagcga
      781 gcggcatctt caaaggcaca ctcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc
      841 tccatctcaa agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg
      901 ctctcttcaa cttccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg
      961 attttatgca gcgggttaat cttaatgccg ggcttactgg agtcgaccca ccacagggtg
     1021 aaggcttttt tgggatcttt cggttgcgga tcgcgcgcca gcaccagcat atacgggtac
     1081 tctttcgcac cggtaataaa ggttttctgt ccgttgatgt aaaccttgcc gtttttacgc
     1141 gtgtaagtgg tagtggcact gttgttatct gagccagcac ctggttccgt caacgccagg
     1201 gcataggcgg gatcacccgt ttccagggtg ctttctgccg ttttacgtag ctgctctgca
     1261 gaaccgaaac ggcgcatact gtgaatacat tgaccgttgg taattaaaaa tgccggagca
     1321 ccgcattttg acacttccat cagcgccagc atttgggtga cgtaatcagc agggatacca
     1381 ccaaattctt ccggcacgcc aagcatggaa ataccgttat ccgccagtgc ccgcataaac
     1441 tcacgcggat atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc
     1501 gtaatcagtt cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatcc
     1561 atcgtgatac tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat
     1621 aaataatctt tcgtccatca gtttgagttc tggcgaaatc actggggtga aatccatttt
     1681 gtcgagaata tctttttgta aatcgacgcc aggggcgatt tcgattaaat gcagtccgtc
     1741 ttctttcagc gtgaatacgg cgcgctcagt gatataacga acatccagcc ctcgctcgag
     1801 agcgattttt ccgctaaaag taatttccgg tagttcccga ataaatttct tcacccgtcc
     1861 ttcctggacg atattcaatt tgccatcggc aatttctgtt tttaaactgc ccgcagttaa
     1921 cgtgccgcag aaaataattt tcttcgaggt ggcactgata tcaataaatc caccggtacc
     1981 catgattttg ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa
     2041 actcaaataa caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc
     2101 cagaatggca cgggtattca cgttcgagcc aaaggcgata ccctgtgaag taattccacc
     2161 aatcggacct gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgggcgac
     2221 caggccaatg ccgtcagcaa taccgacgcc gacattcccc accgcgcctt tacgcatttc
     2281 gaataatgca cgccgtgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtgct
     2341 gtcatcaagg gtgaagtcac cagaaataaa gcggttaacc ggcgcaccgc catatagttg
     2401 gctttgatcc ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt
     2461 aggatgcagt gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt
     2521 attgtgcacc gcctgggcaa taaccaatgc gtcgagatac atcacctcat cttcaaaagt
     2581 ggcgtagcct tcactgtcgc aggtggtagc gcgaatgaaa gcaatatctg gcgcaatcgc
     2641 tttgtaatag agatattctt tgttatcaaa ctcgaccagt ttaatcaggt cttctttagt
     2701 gacttcattc agtttgccgc cttgctggcg tggatcgaca aatgtcccga tgccaatatc
     2761 actaataata ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg
     2821 tggatagtta taagcaataa ttttattttg ttctgcgaga tcagaaatac gcggcgattg
     2881 tccccagtga ccgcataatg cccatttcac cagaccttct tgcgccagcg gactaatacc
     2941 gcggtcggcg cgatcgccaa gccctgttgg actaataatc gataaattac gtggcgtttg
     3001 agtctgttta tatttatcag caagagcagt aattaacgtg gtggcttcca gaataccgcc
     3061 gccagcgcct aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc
     3121 cgacaggacc ggcactcgtc cattaatacg aggtggtttt acaggtttca ttgttaattc
     3181 ctgattcagg tgtgattttc cctacgcc
//
LOCUS       DAZKFZ010000321         3168 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000321 DAZKFZ010000000
VERSION     DAZKFZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            31..1074
                     /locus_tag="ACHOQW_004681"
     CDS             31..1074
                     /locus_tag="ACHOQW_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9575593.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDKD
                     TDTELNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQR
                     AAVWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKG
                     GSHAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAHGGGVFEPLDSEERGR
                     TFEALDCPDWCDD"
     gene            1067..1528
                     /locus_tag="ACHOQW_004682"
     CDS             1067..1528
                     /locus_tag="ACHOQW_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9575594.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            1562..2278
                     /locus_tag="ACHOQW_004683"
     CDS             1562..2278
                     /locus_tag="ACHOQW_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9575595.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASITETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            2311..2592
                     /locus_tag="ACHOQW_004684"
     CDS             2311..2592
                     /locus_tag="ACHOQW_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9575596.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2589..2816
                     /locus_tag="ACHOQW_004685"
     CDS             2589..2816
                     /locus_tag="ACHOQW_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575597.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2809..3120
                     /locus_tag="ACHOQW_004686"
     CDS             2809..3120
                     /locus_tag="ACHOQW_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575598.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          853 a          679 c          986 g          650 t
ORIGIN      
        1 gggtaaagtt cggtatcaga agaggtgagt atggctaatg cctggctcag attgtggcat
       61 gacatgccaa atgaccccaa gtggcgaacg attgccaggg tatcaggaca gccaatcgca
      121 acagtgatgg cagtgtatat ccatcttctg gtgagcgcgt cacgaaatgt cacgacatgt
      181 cacggcgtgt cactacgtgg tcacattgat gtcacgacgg aagatttagc aagtgcgctt
      241 gatgtgacgg aagacgtaat tgattcaatt ttgcatgcaa tgcaggggcg ggttctggat
      301 ggtgacctta tttccggatg ggaaaaacgt caggtgctga aagaggacaa tggtaacgtt
      361 tcgcaaacgg caaaatcccc ggcagagcgc aagagagcgc agcgggagcg ggaaaagctg
      421 cggaaacata atgctgattg tcacgatgag tcacgacgtg tcacgcatct gtcacgacaa
      481 gtcacgacag ataaagatac agataaagat acagatacag aattaaaccc cacacataac
      541 gcgcgcgaga gtattccgac cagtgagtcg aatggtgcgc cgttgcagac agccgaacct
      601 gaatacctgg acggcctgag cgaaccgatc gggaaatttt cgatgactac tgtctggcag
      661 ccgtcgccgg attttcgaca acgggcagca gtgtggggta tggctctgcc tgagccggaa
      721 tttacacctg ctgagcttgc cgcattccgg gattactgga tggcggaggg gaaggttttc
      781 acgcaggttc agtgggagca gaaatttgcc cgccacgtgc agcacgtcag ggcacaggta
      841 aaaccagtca gcaagggggg aagccatgca gcatcaggtg gcacggcatc acgggcagtt
      901 caggaaatcc gggctgcacg cgaacagtgg gaacgtgaca acggatttat cagcaacgga
      961 aacggcctgg aagctgtggg agctcatggg ggaggtgtat tcgaaccgct ggactcagaa
     1021 gaacggggcc gcaccttcga agctctggat tgcccagatt ggtgcgatga ctgaacagca
     1081 aatccgtctg gtgtgccgtc agtgcatgga ccgctgccgg gcgggtgaaa cgtggccccc
     1141 ggacctggct gagtttgttg cgctgatttc ggagagtggg gcaaatccat ttggtcttac
     1201 ggtggatgcc gtgatggaag agtaccggcg ctggcgcaat gaatcctggc gatacgacgg
     1261 gagtgataaa tacccgtggc cacagcctgt gctgtaccac atctgcctcg aaatgcgtac
     1321 cagagggatt gagcgccaga tgacgcaagg tgagttaaaa cgacttgcgg aacggcaact
     1381 gacgaaatgg gcaaagcatg ttggtaacgg gatgagtgtt ccgccagtgc gacgacaact
     1441 ggaaggggcg aaacacccgc aagggccaac gccaattgaa cggctgaaac aggaatacga
     1501 acgccggaag gcagctggtt ttatttgaat ctgagaaacg attttgtcgg aggaaatatt
     1561 aatggaaacc gtatttgacg cactgaaagc actgaaaaaa gcctcttcac aggtagtggc
     1621 atcgcgcctt ggaatcagcc gcgaagatgc tgtcaacgaa ctgtggaaac tgaagcgccg
     1681 tggtgaagcg gataacaagg ggtcgatgtg gtggctgact cagactggtg aaagtgaacc
     1741 agtgtcaccg gtaccgaaag tgacagcgca aatgctgact gaggcgattg aacatcatgg
     1801 cccacaaacg gcggatgagc tggcactgat gttcgggatt acctcccgcc gggcgaattc
     1861 atcactggcc atggcaatca gcaaagggcg tctgattcgc gtgaatcagg gcggtaaatt
     1921 tcggtactgc ataccgggcg ctgatttacc ggcagagccg aaagccgcat ccataacgga
     1981 aacggatggt aaagcctttc ctcagccagc aggtgttgcg ttaccagtcg gggaagcgga
     2041 aacacaggaa gaaataaaaa cggaaagtgt ggcggtcaca gtgcagtcac agccgtcgtt
     2101 caccagaaag catccggatg gtctgatttt accatcgctg catgtggcta accgcgagct
     2161 gcgccgggca aaaggtcagg ttcagaagtg ggagcgagtc tgcgccgcgc tgcgggagct
     2221 gaacaagtgc cgggatattc tccgggatat taccgccacc agagaacagc agcggtgagt
     2281 gggtggaaga cgtggtgccg ggcggaaatc atgatactcc ggcagtgtgc gggaacgatg
     2341 aaggtaaaaa gcgttggcgc acttatcgga cgaactgaag cggcagtgag aacgaaggca
     2401 cgggagctgg gcatcagcat gatgttacgt ggtgattttc acccgtcggc aaaatattct
     2461 cagcgtgata ttgagctggc gcggcaactg catcagagag gcatgcaaag aagggaaatt
     2521 gccagaaaat taggcatgcc gctgcgcata gtgaataact acgtttattt cgacaggagg
     2581 gtgtctgcgt gaaaatcctg tatcaggatt acggcccggt ggggcaggtg gttatcagca
     2641 gtactgtaat ggagtttcgg aagcataacc gtgtggtgga tgctgtgctg ttaacctgtc
     2701 cggggatatc ggcgagtcgt gcaggtgtgt ttattatgaa gacgaaatta tatggcagta
     2761 aggcgtggat aaagaaggcg tatcgtgtag cgttgcagga ggttaacagt gagtgaaatt
     2821 aaagaaatgc cggtagttcg tgacggatat ggctactgga cacatcctga atatgaaaaa
     2881 ttctgtgatg gtcgggaata tatttcaacg gaagagttta acgcctggat ggaggaaaat
     2941 aatcttcaat acgtcctctg cttcagagat gaaggatgtg ctgaccttga tgcgtgtgat
     3001 gctgatattt ctgcatggga accggaacga ccagagggcg atggctggtt tattggttcc
     3061 attcatgata cggaagatgg cccggtttgt gtctggttgc gaaataaggc tgaagcataa
     3121 aggcgataaa ccaactaaca actaaatact gaagatttaa atcagaaa
//
LOCUS       DAZKFZ010000322         3127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000322 DAZKFZ010000000
VERSION     DAZKFZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            27..524
                     /gene="ftnA"
                     /locus_tag="ACHOQW_004687"
     CDS             27..524
                     /gene="ftnA"
                     /locus_tag="ACHOQW_004687"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP9575599.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(561..800)
                     /locus_tag="ACHOQW_004688"
     CDS             complement(561..800)
                     /locus_tag="ACHOQW_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP9575600.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            992..2203
                     /gene="tyrP"
                     /locus_tag="ACHOQW_004689"
     CDS             992..2203
                     /gene="tyrP"
                     /locus_tag="ACHOQW_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP9575601.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2265..2930)
                     /gene="yecA"
                     /locus_tag="ACHOQW_004690"
     CDS             complement(2265..2930)
                     /gene="yecA"
                     /locus_tag="ACHOQW_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP9575602.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT          721 a          778 c          750 g          878 t
ORIGIN      
        1 aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa aaacttaatg
       61 agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc gcctggtgca
      121 gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag gaagagatga
      181 cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg cgtattaata
      241 ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag gaaacctata
      301 aacacgaaca attaatcacc cagaaaatta acgaactggc tcatgctgca atgaccaatc
      361 aggactaccc aacatttaat ttcctgcagt ggtatgtttc tgagcagcat gaagaagaga
      421 aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc gaaggtctgt
      481 attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct ccggcggcag
      541 gtttctgccg ccatttcaaa tcagtgacgg caaatcttac tctgatcggt cgaaaaacct
      601 tcttctgaag gtataaattt gccttttgct gccagaaacg ctaccagctc tcccgctgtc
      661 atcccttctg ccgagcaagt gtgaaaacgt gcctgttcac caaaacgcgc tttaatcgca
      721 gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt taacacttca
      781 tgaccgtgaa tagagtccat cgttcctcct caaaaaaaag cctagcgtag cgattgccgc
      841 ttatgaagac tttgcgccag cgcaggactg aatgcttttt attgtacatt tatgtttaca
      901 ccatatgtaa cgtcggtttg acgaagcagc cgttatgcct taacctgcgc cgcagatatc
      961 actcataaag atcgtcagga cagaagaaag cgtgaaaaac agaaccctgg gaagtgtttt
     1021 tatcgtggcg ggaaccacaa ttggcgcagg catgctggca atgccgctgg ctgcggccgg
     1081 tgttggtttt agcgttacgt taatcttgtt gattgggctt tgggcgttga tgtgctacac
     1141 ggcgctatta ctgctggagg tgtaccagca tgttccggca gataccggtc tgggcacgct
     1201 ggcaaaacgc tatctgggac gctacggtca atggctgacg ggcttcagta tgatgttctt
     1261 aatgtatgct ctgactgcgg catacatcag cggtgccggt gaattgttgg cctccagcat
     1321 cagcgactgg acaggtattt caatgtcggc aaccgctggc gtgttgttgt tcacttttgt
     1381 tgccggtggc gtggtttgtg tcgggacatc gctggtcgat ttatttaacc gttttctgtt
     1441 cagcgccaaa attatttttc tggtggtgat gctggtattg ctgctaccgc atattcacaa
     1501 agtgaatctt ttaaccctgc cgttgcaaca ggggctggca ctgtctgcaa tcccggtgat
     1561 ttttacgtcg tttggttttc acggtagcgt gccgagtatt gtcagctata tggatggcaa
     1621 cattcgtaag ctacgctggg tgtttataac cggtagtgcg atccccctgg tggcatatat
     1681 tttctggcag gtggcgacgc ttggcagcat tgattcaaca acctttatgg gattgctggc
     1741 taatcatgcc ggattaaacg ggctgttaca ggcgttacgc gaaatggtgg cctctccgca
     1801 tgttgagctg gcagtgcatt tatttgctga tttagccctt gccacgtcat ttctcggcgt
     1861 tgcgttaggc ttatttgatt atctggcaga tttgtttcag cgttcaaata ccgttggtgg
     1921 acgattgcaa actggagcaa ttacctttct gccaccgttg gcgtttgcac tgttttatcc
     1981 acgaggattt gtgatggcgc tgggttacgc cggtgtggcg ctggcggtac tggcattgat
     2041 tatcccttcg ctgttgacct ggcaaagcag aaagcacaat cctcaagcgg gttaccgggt
     2101 caaaggtggt cgtccggcgc tggtggtggt gtttctctgt ggtattgctg tgattggcgt
     2161 gcaatttttg attgcggcag ggttgttacc agaagtgggg tgattagata gcctcaaatt
     2221 ccttattggg tgccagaatt aacgctgaca cccaatttga cttcttaatg caggcagcac
     2281 tgcttaaatt ttttaccact accgcacggg caaggatcgt tacgccccgg tttctcttct
     2341 gctttgatcg gttgctgaac agctttttcc tgcggatgcg ccatccagta cgcatgtaga
     2401 tcaagcgccg ccagtcgaat ggcatctacg ctctcttcaa acgcttctgg cgacatcttc
     2461 tctacccgct cgaagttttc ctcagtaccg tgcagcgcta tcgcctccag cgctggtttt
     2521 aacgaatcgg gcaatgttga ccagtcagaa agtgccacgc cccgcatata gccaaagcac
     2581 cactcctcaa caatcgtcag ctcgctgcca tcaacttctc gcaagccgaa taacggctca
     2641 aactgctccg ggaattcgtt cagacgctct gcggtatcgg ccatatgttg aaaagccagg
     2701 ttcataaagc gcgtcatctc tttctctgac gcccagcgcg gcacatagtc agccccaccc
     2761 cacacggcaa ccagccactg tgccggttca atctcttgcg gagaactcaa caccgccgtc
     2821 aataaaccgt ccagctccgc cacatcaagg atggcgtggt cagtgttgta tttggtcaga
     2881 atatcgtcca gccattccaa ctcactttcg tttaacggtc ccgttttcat acgcttttcc
     2941 ttgtggatct caactcgcca gcacctatct tacatgccgg tccgtatcag agatactttt
     3001 tgagtggctt tgccggtgat taaaaatcaa ggagggtgta acgacaagtt gcaggcacaa
     3061 aaaaaccacc cgaaggtggt ttcacgacac tgcttattgc tttgatttta ttcttatctt
     3121 tcccatg
//
LOCUS       DAZKFZ010000323         3120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000323 DAZKFZ010000000
VERSION     DAZKFZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..589)
                     /gene="dsbA"
                     /locus_tag="ACHOQW_004691"
     CDS             complement(<1..589)
                     /gene="dsbA"
                     /locus_tag="ACHOQW_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP9575603.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQ"
     gene            complement(606..1592)
                     /gene="srkA"
                     /locus_tag="ACHOQW_004692"
     CDS             complement(606..1592)
                     /gene="srkA"
                     /locus_tag="ACHOQW_004692"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP9575604.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1669..1938)
                     /locus_tag="ACHOQW_004693"
     CDS             complement(1669..1938)
                     /locus_tag="ACHOQW_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP9575605.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2008..2592
                     /gene="mobA"
                     /locus_tag="ACHOQW_004694"
     CDS             2008..2592
                     /gene="mobA"
                     /locus_tag="ACHOQW_004694"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP9575606.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2574..3101
                     /gene="mobB"
                     /locus_tag="ACHOQW_004695"
     CDS             2574..3101
                     /gene="mobB"
                     /locus_tag="ACHOQW_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP9575607.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          816 a          761 c          757 g          786 t
ORIGIN      
        1 actgctgaac aaaaacatcc atattgctgg tatccatacc ctgcggattc agctgatatt
       61 taccgttaac aaacatcgcc ggaacaccac gcaattgcac gtcagctgca gctttttcct
      121 gctgagcgac cagagatttc accacgaagc tgttccacgc cgcgtcgtac tcttcacctt
      181 taatacctgc gttgataaat acatcgcgga tatcagatgc tgaacgaatg gtctgggttt
      241 tctgtacgcc ttcaaacagc ggaacagtca ctttgtcttc cacgcccagc gccatcgcca
      301 cagcccatgc ctgagtcaga tctttgccca ggtcaccacc catgaagttg acgtggtatt
      361 tagtcatctt cacgccttcc ggcagttttt tcttcacatt atcagaaata tgcagaactt
      421 cttcaaactg atagcagtgc gggcagaaga aagagaaaaa ctccagcact tgcggcgcgc
      481 cagctaccgg tttttccagg gtagtgtact gtttaccatc ttcatactgc gccgccgatg
      541 cgctaaacgc taaaactaaa ccagccagcg ccagccaaat ctttttcatg atctactctc
      601 tccgattaat acataggtgt taattgcaaa gggggttctt gtagaacttt tgcctgttct
      661 ataaaagtcg ccgtctgtcg tagccagtaa tcttccccgg ttaaccacgg gaaatttttc
      721 gggaacgcgg gatcagccca acgccgcatt agccaggcaa gataataaac caaacgcatg
      781 gcgcgtaaag gttcaatcag tccgatttca gcggtgtcga actcgctaaa ttcttcataa
      841 gcttcaataa tagtttccag ttgcatccgc tgctcggctt tatcgccatt gagcaacatc
      901 cacaaatcct gaatagctgg accattacgt gcatcatcca gatcaacaaa cattggacca
      961 tcgcgccaga gaatattccc ggcgtggcag tctccatgta gccgcagaac ggtgaaatct
     1021 tcccgccagt gtgctgtaac ggcggcgatc agctcatctg tcgctttcag gaatgccgct
     1081 ttcaacccgg aaggtatcag tgtagcgtcc tcaaacagct tgcgtggctc aatgagatat
     1141 tcattcagac cgatggtcgg gcgatggata aaaagctgtt tgcgccccgt ctggtgcata
     1201 cgccctaaat aacgcccaac cgcttccatc tgatcgatat tatcagcttc gaactggcga
     1261 ccaccgacgc ttggaaaaac agcgaaataa aatccctgat gattcaataa agtctgaccg
     1321 ttaaaagcca caggtgctgc aaccggaact tcatcattta ccagctgcaa cgcaaattga
     1381 tgttcttcga ggatttgatc ggctgtccaa cgttcaggac gataaaattt gacgacaaaa
     1441 cgtcgacgat cttcgtcctg aaattgatag acacggtttt catagctgtt aagcggggta
     1501 agaccggaat ccacccggat cccttgctca aacagagcgt ccatgatggt atccgggtgt
     1561 agtgtctgga aagtaaaagc gctgttgttc atcccatcat ccggaaaaca ttacgaatga
     1621 tgaaggatat catttcacga tgattttggt ggcgccgctt acaagctttt actctttaat
     1681 tacgccgcga gcgcgtaaca gcgccgtttt gaagtcttct tcataatctt tctgcaaacc
     1741 cgggatcacc gcatctttcg cggaatcacg cattttcagg tgatagatca gaatgtcatc
     1801 cgtcaaatcc gccagttcgc cgtcaaaacc tgactctttc gccagtttct gcaaaaattg
     1861 caacaggtta aggtctggct ctttttgcca ggctggctgg aggagttcaa taacttcatt
     1921 cagacgttta catttcatag taatgctcct tactcttgag acagacacgt tagcagggtc
     1981 aatcccacaa taaaagaggc gatatcggtg aatctgatga cgacgataac aggcgttgtg
     2041 ctggcaggcg gtaaagccag acgaatgggc ggcgtagata aaggattgct ggacttaaat
     2101 ggcaaaccgc tgtggcaaca tgtcgctgac gcgcttatga cgcagctctc tcacgtcgtg
     2161 gttaatgcta atcgtcatca ggaaatctat caggcaggcg gtctgaaagt gattgaagat
     2221 tcactggcgg attacccagg ccctctggca gggatgcttt cagtaatgca gcaggaagct
     2281 ggtgagtggt ttttgttttg tccgtgcgat acgccttaca ttccccatga tttagccgcc
     2341 cggcttaatc atcagcgcaa agatgcgcct gtcgtgtggg tccatgacgg tgaacgcgat
     2401 cacccgacta ttgctatggt aaaccgcgct attgagcctt tattactaga atatctgcaa
     2461 gcaggagaac gccgggtaat ggcatttatg cgtctggctg gcggtcatgc ggttgatttc
     2521 agcgatcata aagatgcatt tgttaacgtg aatacgccag aggagcttgc ccgatggcag
     2581 gaaaaacgat gataccgtta ctcgcctttg ccgcgtggag tggtaccgga aaaactacgc
     2641 ttttgaaaaa actgatcccg gcattatgcg ccagagggat ccgtccaggg ctgattaagc
     2701 atacgcacca tgatatggat gttgataagc caggcaaaga tagctatgag ctgcgcaagg
     2761 ctggcgcggc acaaaccatc gttgccagcc agcaacgatg ggccttgatg acggaaacac
     2821 cagacgaaga agagctggat ctacagtttc tcgcaagtcg aatggatacc tcaaagctgg
     2881 atttgattct ggtcgaaggg tttaagcatg aagagatcgc aaagattgtg ctgtttcgcg
     2941 atggagccgg acatcgacct gaagaattag tgatagacag gcatgttatt gctgtagcca
     3001 gtgatgtgcc gcttaatctt gatgtcgcat tactggatat taatgatgtt gaggggctgg
     3061 ctgattttgt ggtggagtgg atgcaaaatc aggacggata agcaagagta ggctggagca
//
LOCUS       DAZKFZ010000324         3111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000324 DAZKFZ010000000
VERSION     DAZKFZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            64..1746
                     /gene="ydeN"
                     /locus_tag="ACHOQW_004696"
     CDS             64..1746
                     /gene="ydeN"
                     /locus_tag="ACHOQW_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9575608.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQVPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1798..2955
                     /gene="ydeM"
                     /locus_tag="ACHOQW_004697"
     CDS             1798..2955
                     /gene="ydeM"
                     /locus_tag="ACHOQW_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9575609.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2921..3043)
                     /locus_tag="ACHOQW_004698"
     CDS             complement(2921..3043)
                     /locus_tag="ACHOQW_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575610.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          937 a          674 c          639 g          861 t
ORIGIN      
        1 catccactta tttctcttcg taaaattact ttggaattaa gtacaataag aagaggaaca
       61 tttatgaagt ctgcattaaa gaaaagtgtc gtaagtacct cgatatcttt gatactggca
      121 tctggtatgg ctgcatttgc tgctcatgcg gcagatgatg taaagctgaa agcaaccaaa
      181 acaaacgttg ctttctcaga ctttacgccg actgaataca gtaccaaagg aaagccaaat
      241 attatcgtac tgaccatgga tgatcttggt tatggacaag ttccttttga taagggatct
      301 tttgacccaa aaacaatgga aaatcgtgaa gttgtcgata cctacaaaat agggatagat
      361 aaagccattg aagctgcgca aaaatcaacg ccgacgctcc tttcattaat ggatgaaggc
      421 gtacgtttta ctaacggcta tgtggcgcac ggtgtttccg gcccctcccg cgccgcaata
      481 atgaccggtc gagcgcccgc ccgctttggt gtctattcca atactgatgc tcaggatggt
      541 attccgctaa cagaaacttt cttgcctgaa ttattccaga atcatggtta ttacactgca
      601 gcagtaggta aatggcactt gtcaaaaatc agtaatgtgc cggtaccgga agataaacaa
      661 acacgtgact atcatgacaa cttcaccaca ttttctgcgg aagaatggca accgcaaaac
      721 cgtggctttg attactttat gggattccac gctgcaggaa cggcatatta caactcccct
      781 tcactgttca agaatcgtga acgtgtcccc gcaaaaggtt atatcagcga tcagttaacc
      841 gatgaggcaa ttggcgttgt tgatcgtgcc aaaacacttg accagccttt tatgctttac
      901 ctggcttata atgctccgca cctgccaaat gataatcctg caccggagca atatcagaag
      961 caatttaata ccggtagtca aacagcagat aactactacg cttccgttta ttctgttgat
     1021 cagggtgtaa aacgcattct cgaacaactg aagaaaaacg gacagtatga caatacaatt
     1081 attctcttta cctccgataa tggtgcggtt atcgatggtc ctctgccgct gaacggggcg
     1141 caaaaaggct ataagagtca gacctatcct ggcggtactc acaccccaat gtttatgtgg
     1201 tggaaaggaa aacttcaacc cggtaattat gacaagctga tttccgcaat ggatttctac
     1261 ccgacagctc ttgatgcagc cgatatcagc attccaaaag accttaagct ggatggcgtt
     1321 tccttgctgc cctggttgca agataagaaa caaggcgagc cacataaaaa tctgacctgg
     1381 ataacctctt attctcactg gtttgatgag gaaaatattc cattctggga taactaccac
     1441 aaatttgtcc gtcatcagtc agacgattac ccgcataacc ccaacactga ggacttaagc
     1501 caattctctt atacggtgag aaataacgat tattcgcttg tctatacagt agaaaacaat
     1561 cagttaggtc tctacaaact gacggatcta cagcaaaaag ataaccttgc cgccgccaat
     1621 ccgcaggtcg ttaaagagat gcaaggcgtg gtaagagagt ttatcgacag cagccagcca
     1681 ccgcttagcg aggtaaatca ggagaagttt aacaatatca agaaagcact aagcgaagcg
     1741 aaataactaa accttcatgc ggcggatttt tccgccgcct tattgagcga gatagcgatg
     1801 cacgttacag ccaagccctc cagttttcaa tgtaatctca aatgtgatta ctgtttttac
     1861 cttgaaaaag agtcgcagtt tactcatgaa aaatggatgg atgacagcac tctgaaagag
     1921 ttcatcaaac aatatatcgc agcgtctggc aatcaggtct attttacctg gcaaggcggt
     1981 gaacccactc tggctggcct ggattttttc cgtaaagtta ttcactatca acaacgctat
     2041 gcaggccaaa aacgtatttt taatgcatta caaacgaatg gcattttatt gaataatgaa
     2101 tggtgtgcct ttctcaaaga acatgagttt ctggttggta tctcgatcga tggaccccag
     2161 gagctacatg accgttacag acgcagtaat tcaggtaacg gtacttttgc aaaagtgata
     2221 gcagccatcg agcgtctgaa atcatatcaa gtagagttta atacgttaac cgtcattaat
     2281 aacgttaatg tccattaccc tcttgaggtt tatcattttt taaaatctat cggcagtaaa
     2341 catatgcaat ttatcgaatt gctcgaaacc gggacgccga atattgattt cagtggtcat
     2401 agtgagaaca cattccgtat cattgatttt tctgtgcctc ccacggctta tggcaagttt
     2461 atgtcaacca tttttatgca atgggttaaa aacgatgtgg gtgaaatttt catccgtcag
     2521 tttgaaagct ttgtcagccg ttttttgggg aatgggcata ccagttgtat tttccaggag
     2581 tcctgcaagg ataatctggt tgttgaaagt aatggagata tttacgaatg cgatcatttt
     2641 gtctatccac agtacaaact tggaaacatt aataaatctg aacttaaaac gatgaacagt
     2701 gtacaactga cagcgcaaaa aaaacggatt tcagcgaaat gtcagcaatg tgcatataaa
     2761 cctatctgca atggcggttg ccctaagcat cgtattacta aagtaaacga tgagactgtt
     2821 tcctattttt gcgaaggtta taaaatcctt ttttcaacca tggtacctta tatgaacgcc
     2881 atggtggagt tagccaagaa cagagtaccg ctttaccaca ttatggatgt tgcaaaacaa
     2941 atggagaata attaataacg ctctctttta gctccattct tagcctcctt cttatgacct
     3001 taacaaggca cccgacataa attcctgtcg ggtgtttcgt catcatcatc ttcccttgcg
     3061 aaaatcttgg cttacgtggt atgcgtcagg gcattagcaa tacccttgat t
//
LOCUS       DAZKFZ010000325         3099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000325 DAZKFZ010000000
VERSION     DAZKFZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..282)
                     /locus_tag="ACHOQW_004699"
     CDS             complement(<1..282)
                     /locus_tag="ACHOQW_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP9575611.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFE"
     gene            complement(295..2745)
                     /locus_tag="ACHOQW_004700"
     CDS             complement(295..2745)
                     /locus_tag="ACHOQW_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9575612.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            complement(2758..>3099)
                     /locus_tag="ACHOQW_004701"
     CDS             complement(2758..>3099)
                     /locus_tag="ACHOQW_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9575613.1"
                     /translation="NPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKN
                     NAISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDY
                     GNNIHSKIAAR"
BASE COUNT          854 a          620 c          619 g         1006 t
ORIGIN      
        1 ttcaaagggg aaattaataa tataatgacg acttccgact ttaaacctta aagcgttctg
       61 tgttaaaaaa tgaactttaa tggcgttggt cgtctcttgc aagtctgcac actcaatgat
      121 attcccagta ttttcaattt tttcggcggt gagattacct ttcatactca gtggtttgag
      181 tatatatgtt ccacgttgtt ccccgacatt ggtcaatcca cagggagcac tttgcacaat
      241 aaaagaatat agactcaatg tgattaataa aagccacttc atagtaaact ccttttactg
      301 gcatatatat gttttgtttt catcgataac gtgttgaaaa gtaatagtac agaataaatt
      361 ctgttcatca ttgagagcaa cttttatgcc agttggaatt tcatctacgc gaataaaaag
      421 gcgactaccc tggccaacga ctccaatatt gttttcctgg agatccagta cgtcatagcc
      481 aaaagttaat ggcgaaccat cgggacgcag tgcctgtata taccagggct tacgttggtc
      541 agttgtaaac tgcacataag aaactgctcc acggtaaggc gcaataattt gacgattgcc
      601 ctgcaattct gtttcacttt cactgtttgc tgtatcaagt accagatggt tttcacgatg
      661 aggtatcatc aggtcgtaaa caacctggcc tttgctgttt gttcggttgt atttttgtcc
      721 attaatatgc gcgccttcta acccaggcgc atccagaatt gcaaatgtat cggatagctg
      781 gttagtgata ttaataccac cgggccagac aactaatcca ctactaatac ttccaccact
      841 ttgccaggcg tttttagaat ggctatagct gccatctatt gtggcgatgg gagttctcca
      901 gcttaaattc gtaccaagcg atgtatcatt attttgttgt tgctgattaa catatatacc
      961 ataatttaac tgatcatgtt caccggctat gccagtaatt ccagtattgt tggaggaata
     1021 gccatctttg gaaaatgagg tcgaattcga taagttaatt tggtgacgtg ttttggcaat
     1081 atcatccccc cagtagaaag gaatggagat aaacagatta aaacgctcct cttctttgtc
     1141 gttttcatca taggattggc tggcagagaa agtataactg atgcgttgcc agctattgga
     1201 ataactgaat tggtaatctt tagcatttcc acttcgcccc cagtaattcc gccaaagcgc
     1261 acttaatgat acatttccca gattattgga taaaggttgc atgatattgg cagaaaggct
     1321 attttttctg ccaatatcat aaaaatcgtc atagtcactc tgatgattta ttttatcatt
     1381 ttcgtaaaga tggtcgctga atgtcctgta atcctgcgaa gcataacgcc aggcggccac
     1441 gctaaatcga gtggccgttt gtactagata tttattatag gcgacctgat agctcgtacc
     1501 ttcgtgtgtt atatcattat ttaacttact actcgatcgt gtagcgtcaa aagagatggc
     1561 acccagcggt gtattccaac catttcccag agttatagca ttatagttat cggataaaat
     1621 cgtaccgcca taaagtgtca aaagattgtt gaggccatat atataatttg cctcgagaaa
     1681 gtcctcctga ttctttacac catatatctt actgcgtccg gcgataaaat cgaaatttga
     1741 aataccaggt tgcagcatgt taggcacgga agagtagggc accaaaaagg aacgaacact
     1801 gccatctgct tcctttatgc tgacatcgag atcagagccg ctgcccgaga gttgcaaatc
     1861 tgcgatagta aatggtcccg ggggaacctc tttttggtaa atgatgtagc cattttgcga
     1921 aaccgttatc aaggcattac tttgcgcaac accttgtact aaaggtgtaa agctctgcat
     1981 agaatcaggt agcatttgca tatcgcgaaa taagcgtatt ccgctaaatc tcagagagtc
     2041 gaatatgagt gaggatgtgt aattttctcc tatctgaact gtgcttaata tctgcgacca
     2101 gccatgttcc aggtaaagag tattactttg ccatgtgcct ttcatatcat cgggtttgct
     2161 atagctggca tcagagtgta actgccaact aaataaattt aatccactgt taaatcggcc
     2221 ataactggct gtattactgt tattattgct gtcataagag cgatactgac ttaaattata
     2281 ggaggtataa aaagcatcaa tgccgcgatc ccaactttcc gggggaacgt aacctctttc
     2341 aagttcattg atatacgcct gtggtactgt taaatttagt cggtgttcac tgatatccca
     2401 ctgatattgt ccaccatgaa ccgctttatc cagtaaaata caattatctt ctgtattgag
     2461 gttaccggtc tttaccccca gtttggttag tagcacctta ggcagacatg gtttgactgg
     2521 tgactctggg atcgtgatat cttgttttcc acgccactgg ttatttacat aaaaatcaag
     2581 ttcgtagttt cctggaagcg gttgtttatt atcgaaatga tattcggaaa ctttttctcc
     2641 tttcatgccg ccgatcataa agtgcgtatc aaaagtttct tcagcataac ccggaaaaga
     2701 atacaaaaaa ataatagcag cgacccatcg tgtcgtcctt aacatgactt tttcctgtta
     2761 tctcgcggcg attttactat ggatgttatt cccgtaatca tcaactaccg ttagttcata
     2821 ttctgatgcg tggttatttt ttaatgtaat gtcgttatgc gaaaagggag gcagtaagat
     2881 actttcatta ttaactttca cgttttgtgc tttgatagtt gtaaccgtta tccagttggc
     2941 tgtttcgttg tttattgaga tggcgttatt tttctttttg atatttaatt gagaaaggct
     3001 ttttttatct accggagcta ttccgcttgg acgccaaagt aactttatcc ggttttgcaa
     3061 tgcaagttta attttgtttt tccctgcatt ttgtggatt
//
LOCUS       DAZKFZ010000326         3093 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000326 DAZKFZ010000000
VERSION     DAZKFZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..662
                     /locus_tag="ACHOQW_004702"
     CDS             <1..662
                     /locus_tag="ACHOQW_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFP9575614.1"
                     /translation="VPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNLLERQ
                     FAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMA
                     YEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLK
                     TEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVASI
                     T"
     gene            671..823
                     /locus_tag="ACHOQW_004703"
                     /pseudo
     CDS             671..823
                     /locus_tag="ACHOQW_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            913..1287
                     /locus_tag="ACHOQW_004704"
     CDS             913..1287
                     /locus_tag="ACHOQW_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9575615.1"
                     /translation="MKTLPDTHVREASRCPSPITIWQTLLGRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAK"
     gene            1284..1775
                     /locus_tag="ACHOQW_004705"
     CDS             1284..1775
                     /locus_tag="ACHOQW_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9575616.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFTALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGLPT
                     FNW"
     gene            1787..1984
                     /locus_tag="ACHOQW_004706"
     CDS             1787..1984
                     /locus_tag="ACHOQW_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9575617.1"
                     /translation="MTSLTPEAALDILIAWLQDNIDSESGIIFDNDEDKTDSAALLPC
                     IEQVREDVRTLRQLQLLQQNR"
     gene            2069..2410
                     /locus_tag="ACHOQW_004707"
     CDS             2069..2410
                     /locus_tag="ACHOQW_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575618.1"
                     /translation="MSGIIISRPEVDTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAADIGGKTALDRAMKQDFHCGCWLMEKPETAMKAITRNLDCEIWRDLMQR
                     SGMLSLMDAQG"
     gene            2471..2614
                     /locus_tag="ACHOQW_004708"
     CDS             2471..2614
                     /locus_tag="ACHOQW_004708"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575619.1"
                     /translation="MNAFKQLHQSKDEVFERGVINVFRGPNGITKPIPPVNSAVKLSS
                     TTW"
     gene            2608..3027
                     /locus_tag="ACHOQW_004709"
                     /pseudo
     CDS             2608..3027
                     /locus_tag="ACHOQW_004709"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT          830 a          794 c          756 g          713 t
ORIGIN      
        1 gcgtcccgat gagccgttac cgtgccgggc gtctgatgaa atatctgaac ctgagcagtt
       61 gtcagcccgg aaaacatcag tacaaaaatg ctcgtcagga acatacctgt ctgccgaatc
      121 tgcttgagcg ccagttcgct gtgcccgagc cagatagggt atggtgcgga gatattacgt
      181 atatctgggc aggaaatcgc tggtgctatc tggcggtcgt catggatctt tttgcccgca
      241 gggttatcgg ctggagcctg tcagcgaatg ccgatactgc cctgataagc agtgccctgc
      301 ggatggcgta tgaggtgcgt ggtcagccgc gggacgtcat gttccatagc gaccagggaa
      361 gtcaatatac aggactgaaa tatcaacaac ttctctggcg ttacaggata aagcaaagtg
      421 tcagccgacg gggaaactgc tgggataaca gtccaatgga acgcttcttc cgcagtctga
      481 aaacagaatg ggtgccaacc gatggctaca caggcaaaga cgtggcccgg cagcaaatca
      541 gcagttatat tctgaattat tacaatagcg tcaggcctca ccattataac ggagggctga
      601 cgccggaaga gtcagagaac agatatcatt tttactgtaa aaccgtggcc agtattactt
      661 gaccactaca ctggaactca tgctcaccag cggtgagctg aatccccgcc atcagcatac
      721 cgtcacgctg tatgcaaaag ggctgacctg caaagccgat accctcggca gtcgaggtta
      781 cgtttatatg gctgtttatc cgacgcccga aacgaaaaag taaatcatca gaataacctt
      841 ctgctccggc ctggtgtttt caccacgcca cttccccatt tctcatctct gcatatcagg
      901 aaaaacttta gtatgaaaac attacccgat acacacgtac gggaggcatc gcgctgcccg
      961 tctcccatca ccatctggca gacactgctt ggccgtctgc tggaccagca ctacggcctc
     1021 acgctgaatg acacaccgtt cgctgatgaa cgtgtgattg agcagcatat tgaggcaggt
     1081 atttcactgt gtgatgcggt gaactttctc gtggaaaaat acgtgctggt gcgtaccgac
     1141 cagccgggat tcagcgcctg tacccgctct cagttaataa acagcatcga tatcctccgg
     1201 gctcgcaggg cgaccggcct gatgacccgc gacaattaca gaacggtaaa taacattacc
     1261 ctgggtaagc atccggaggc gaaatgatga aactggccct gacactggaa gccgacagcg
     1321 ttaacgtaca ggcactgaat atggggcgaa ttgtcgttga cattgatggt gttaatctcg
     1381 ctgaactgat taacatggtc tgcgataacg gctactccct tcgtgttgtt gatgaatctg
     1441 accggacctc agcagactgc acgccaccat ttactgccct taccggcata cgctgcagta
     1501 ccgcacatat cacggaaacg gacaacgcct ggctgtactc gctgtcacac cagaccagtg
     1561 acttcggtga atcagaatgg attcatttta ccggtaacgg ctatctgttg cgtaccgatg
     1621 cgtggtcgta cccggttctg cggcttaaac gcctgggtct gtcaaaaacg ttccgtcgtc
     1681 tggtcgtcac actcatccgg cgttatggtg tcagtctcat tcatctggat gccagtgccg
     1741 gatgtctgcc aggtttaccc acttttaatt ggtaactagg aacaacatga catcattaac
     1801 cccggaagca gcactggata ttctgattgc gtggctgcag gacaatatcg acagcgaatc
     1861 cggaattatc ttcgacaacg atgaggataa aacggattcg gcagcattgt tgccctgtat
     1921 cgaacaggtc agggaagatg tccgtaccct gcgccaactg cagcttctgc aacagaaccg
     1981 gtgagtctca ctcaccatac ttcattccac tgacgccagc ctgaacaggg ctggcgtttt
     2041 cgtttataaa ccaaaaggaa aaccgataat gtcaggcatc ataatctccc gtccggaagt
     2101 ggacaccggg catacggacg ttatctgctc caccagtatc cgacacgttg tgacggtacg
     2161 gaatgcagca ctgcaacaaa ccgaaacgct tatccggcaa ctggcagaaa tctcagtcct
     2221 gacagcagca gacattggcg gcaaaaccgc cctggaccgg gcgatgaaac aggattttca
     2281 ctgcggctgc tggctgatgg agaaacctga aaccgcaatg aaagccatca cccgcaacct
     2341 cgactgcgaa atctggcgtg acctgatgca acgctccggg atgctgagtc tgatggatgc
     2401 acagggctag gatacatggt accgctcact ggagtacgat aatattccgg aaatcagcga
     2461 ggcgaatatc atgaatgcat tcaaacaact gcaccagagt aaggatgaag tgtttgagcg
     2521 tggggtgatc aacgtcttca gagggccgaa cggaattaca aaaccaattc cccctgtaaa
     2581 ttcggcagta aaattatcgt caacaacttg gtgagatggg acagatggag atttcatctt
     2641 atcagtggaa tgcaggcaga tcgcttagct gacctggaaa gaatgttgca tctgctcagc
     2701 ggtaaaccgc tccccaacaa ccgagggaat atcaccatta atctggatga ccacctacag
     2761 tccgttcagg gtaaagagcg ctatgaagac aagtaaatac tttaagaagg gcccgcacac
     2821 atcacattca gaaagccaga gctggttgac aggcttaatg atattattgc cagacactac
     2881 ctaggaatgt taccttcaca aaaatctcta agaattgatt caattgtgta ttttagcctc
     2941 attccctcat gtaaagtcat ttgggggtta ttatgggggt caatcattta tccaacacgc
     3001 attaatcatt taaaatcaat gcattaataa tcatattcga ctcctgtgat ctccgccaaa
     3061 cttcctcttc tgaactctcc cgacgtcaac aaa
//
LOCUS       DAZKFZ010000327         3043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000327 DAZKFZ010000000
VERSION     DAZKFZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..359
                     /locus_tag="ACHOQW_004710"
     CDS             <1..359
                     /locus_tag="ACHOQW_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9575620.1"
                     /translation="GPKGDTGAAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQI
                     RFRLGPGNIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQD
                     VKGLDEVGSDTGRTGE"
     gene            361..630
                     /locus_tag="ACHOQW_004711"
     CDS             361..630
                     /locus_tag="ACHOQW_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9575621.1"
                     /translation="MNVLKKLMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            891..1168
                     /locus_tag="ACHOQW_004712"
                     /pseudo
     CDS             891..1168
                     /locus_tag="ACHOQW_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
     gene            1418..1543
                     /locus_tag="ACHOQW_004713"
                     /pseudo
     CDS             1418..1543
                     /locus_tag="ACHOQW_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1603..2109)
                     /gene="yciE"
                     /locus_tag="ACHOQW_004714"
     CDS             complement(1603..2109)
                     /gene="yciE"
                     /locus_tag="ACHOQW_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9575622.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(2155..2655)
                     /gene="yciF"
                     /locus_tag="ACHOQW_004715"
     CDS             complement(2155..2655)
                     /gene="yciF"
                     /locus_tag="ACHOQW_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9575623.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2741..2920)
                     /gene="yciG"
                     /locus_tag="ACHOQW_004716"
     CDS             complement(2741..2920)
                     /gene="yciG"
                     /locus_tag="ACHOQW_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9575624.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT          805 a          668 c          712 g          858 t
ORIGIN      
        1 agggtccaaa gggagacacc ggggcagcag gcccggcagg cccacaggga ccgaaaggag
       61 aaacaggtgc ggctggcccg gtgggggcaa ccggacctca gggaccgaag ggcgacccgg
      121 gggagacaca aatccgtttt cgtctggggc cgggaaacat tattgagaca aacagccatg
      181 gctggttccc ggatacagat ggcgcactca tcaccggact gacctttctt gaccccaaag
      241 atgccacacg ggttcagggt ttttttcagc atttgcaggt caggtttggt gacgggccgt
      301 ggcaggatgt caaggggctg gatgaagtgg gcagtgatac aggcagaaca ggagaatgac
      361 atgaacgtac taaaaaaact tatgcagcgt ctgtgcggtt gcggaaagca tgatgaccgt
      421 gaaaacgggg agttacttac agcacagctg cgactgggac cggcagacat tctggagtcc
      481 gatgagaatg gcattatccc ggagcaggac agggtaatca cgcaggtggt gatactggat
      541 gcggataaaa agcagataca gtgcgtggta agaccgctgc aaatcctgcg tgctgacggg
      601 aggtgggaaa atattggcgg aatgaaatag ccgacagctt cacaaaaacc ggagtccggc
      661 tccggttttt tgttgtcatg tccggtggat gtttgttagg aatgttcaga caggtttatt
      721 ttgaatttac acagaatcct aaacaggttc gagaattaag aaagaggttg tatgtttagc
      781 ataagaaccc tactacctat tagcgccagc gtatcagttc cgacaaaaca atctcggtaa
      841 tgctgccaac ttactgattt agtgtatgat ggtgatttta aggtgcttgc gtggcttcca
      901 tttccatcag atgtccttcc tgctccgcta ctgaaggcgt ggtgcgtaac ggcaaaagca
      961 ctgccggaca tcagcgctat ctctgctctc cttgccgtaa aacatggcaa ctacagttca
     1021 cttacaccgc ctctcagccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg
     1081 tcggatgtcg cgccagtgca cgcattatgg gcgttggcct caacacggtt ttacgtcact
     1141 taaaaaactc aggccgcagt cggtaacaga gcgatgatga ttggcagtaa ttagcggtca
     1201 atttcgggtt ttttggctgt gcgctccccg cccagcattg cgcgtcaatt agccccaaaa
     1261 aaaacgaaaa agttacaatc aagtaaaaaa ttccttctgc aaaactaaaa aagatttcaa
     1321 taaaatcagg cagttaaaaa cataaaaaaa aggtacaaaa aagcatctca aagtcttgtt
     1381 ttttgcttca aatacaattg gttattgtgt tttttgaaac ctaccagtgt ggtacatgga
     1441 tatcgatacc accgccaatt ataaactggg cggtgcacag caacatgata gcgtacgcct
     1501 cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt taattccgca caaaaacgac
     1561 cccgtaatat acggggtcaa taaggacatg gcataaaggg tattatttct tcgcttctac
     1621 gccatcagtt tcagagcgaa ttaaaaattt ctcagttgtt tgcggaatat gctgaatcag
     1681 ccagtcggcc atttgctttt cctcatttaa aatcgcttcg atgatcggaa ttgaggctgt
     1741 atcaccggca ttttttgctg ctgctaatag agaggtgtaa caggcgattt caaattgctc
     1801 gaagacatat ccgctaatag agcctttgac tatttcatca gaagggaata taccaccgat
     1861 tgactgccca agcgcagcca ttttactcat ggaatcttta atgactgaac gtgaaatgtc
     1921 attacgatca agaatagttt ccagttgaac aatctggttt ttggtttcac taagatgttg
     1981 ttcaatacga gcacgtagtt caggataatt atctatacgg ctggccatgg attcaagcat
     2041 agattcggct tgcttttcca ttgcatgggc gtcacgtaac cagtcatgat aatgttcaat
     2101 acgattcatg aaaatgtcct ctttataaaa tgaccaaaag ttaaagttca tatttcaggc
     2161 tttattttcg gctttcttat ttacgttatt aagggccaga tcagtcagtt tgatgtcggt
     2221 ggccttttct tcttccaggg tctctttcag aagcttcgct gctttacggt aacctaattg
     2281 ttcagccagc gtcgctaatg tcccgtaact ggcaatctca taatgctcga ctttctgtgc
     2341 tgcggcaatc agtgcggcat cacgcacttc gtttttctcg gtactttcga tgacctcatt
     2401 agcttcttca ataagacctt ccattgccac acatttcatg cgcttaattt tcagattcga
     2461 ttctgattcc acaacttgat caatacgttc aatctgtcca tgtgtttcct cgaggtgcgc
     2521 atgaaaagcc tgacttaatt tttcgtttga tgttgctctt gcgagttttg ccagtgcccg
     2581 ggttaattgt ttttctgcgc tgtaggtatc tgaaagcagg tgaataaata catcttcaat
     2641 ggtcttcata ttcatatttt tctccagtga aatcactgcg agccatacgg cccgcagatg
     2701 caattatctt tatcagctga tattaataat taacggtgaa tcaggatttt cctgatttat
     2761 taccaccgct ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc ggatcatttt
     2821 taaaattacc gccgctatgc tgaccgcctt tacggcctgc gtcggatgcc ttctcacggt
     2881 cttcggcgaa atttcctgaa ccaccacgat gttcggccat gttatttctc ccgttgcgtt
     2941 gcattgtttc attaatatga gtgttgtgtg tcgatactca ttaaaattag tcgctaatga
     3001 ggattagtca aattaagcgt aacgagaaga aagtaggaaa ata
//
LOCUS       DAZKFZ010000328         3025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000328 DAZKFZ010000000
VERSION     DAZKFZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>3025
                     /locus_tag="ACHOQW_004717"
     CDS             <1..>3025
                     /locus_tag="ACHOQW_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9575625.1"
                     /translation="SILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSE
                     TALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNG
                     NWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYT
                     EIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWD
                     GSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQRVPDGFGGT
                     EPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVV
                     VDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDA
                     FGCTSRGQAHRAGLWVIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGG
                     RVLSIDAATRTLTLDREVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPD
                     GVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPLS
                     GSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGENSRLLT
                     TAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAPAGVELTPGYF
                     QITAVPKLTIYDPTVQFEFWFSEAKIADAAQVETSARYLGTGSQWSVSGPHIKPGKDF
                     WFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADE
                     MAEMKTSITETRNEITQTVSKTLENQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDG
                     IPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLK
                     RLFAVSITSSGNPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINENCQIKGKLS
                     ANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQ"
BASE COUNT          699 a          824 c          938 g          564 t
ORIGIN      
        1 agagtattct ggtgaacaaa accccgctga cggacacgga cggtaatcct gtgatacatg
       61 gtgtgacagc ggtctggcgc gccggggagc aggagcagac accgccggaa ggttttgagt
      121 catccggctc tgaaaccgca ctgggcgtgg aagtgacgaa ggcaaagccg gtgacgcgca
      181 ccattacgtc cgcgaacatt gaccgcctgc gggttacctt cggggtgcag tcactggtgc
      241 agaccacctc acagggtgac cgtaacccgg catccgtccg cctgctgatt cagctgcagc
      301 gtaacggtaa ctgggtgacg gaaaaggatg tcaccattaa cggcaagacc acctcacagt
      361 tcctcgcttc ggtgattctg gataatctgc ctccccgccc ctttaacatc cggatggtca
      421 gggagacggc ggacagcacc acggaccagc tgcagaataa gacgctctgg tcgtcataca
      481 ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc gattgtgggg ctgcaggtgg
      541 atgcggagca gtttggcggt cagcagatga cggtgaacta ccatatccgc ggtcgcatca
      601 ttcaggtacc gtcaaactat gacccggaaa aacgcacgta cagcggcatc tgggacggca
      661 gcctgaaacc ggcatacagc aacaacccgg cctggtgcct gtgggacatg ctgactcacc
      721 cgcgctacgg catgggaaaa cgcctggggg ccgcggatgt ggacaagtgg gcgctgtatg
      781 ccatcgggca gtactgtgac cagcgtgtcc cggacggctt cggagggaca gagccgcgga
      841 tgacctttaa tgcgtacctg tcacaacagc gtaaggcgtg ggacgttctc agtgatttct
      901 gctcggcgat gcgctgtatg ccggtatgga acggccagac gctgacgttc gttcaggacc
      961 gtccgtcgga tgtggtgtgg ccgtacacca acagcgatgt ggtggtggat gataacggcg
     1021 tggggttccg ctacagcttc agcgccctga aggaccgcca cacggcggtg gaggtgaatt
     1081 acaccgaccc gcagaacggc tggcagacct ccacggaact ggtggaagac ccggaagcca
     1141 tactgcgcta cggacgcaac ctgctgaaga tggacgcgtt cggctgtacc agccgcggtc
     1201 aggcccaccg tgccggactg tgggtgataa agaccggact gctggaaacg cagacggtgg
     1261 atttcacgct cgggtctcag gggctgcggc acacacccgg tgacattatt gaaatctgtg
     1321 ataatgacta tgccgggacc ctgaccggcg gacgtgtcct gtccattgat gctgccaccc
     1381 gcaccctgac gctggaccgt gaggttaccc tgccggagac aggtacatcg gcggtgaacc
     1441 tgattaacgg cagcggtaag ccggtgagtg tggacatcac cgcacacccc gcgccggacc
     1501 ggatacaggt cagcaccctg cctgatggtg tggagacata cggtgtatgg ggactctccc
     1561 tgccgtcact gcgtcgtcgc ctgttccgct gtgtctccat ccgggaaaac acggacggca
     1621 cctttgccat cacggcggtg cagcacgtac cggaaaaaga agccattgtg gataacgggg
     1681 ccagctttga gccactgtcc ggttcgctga acagcgtcat cccgccggct gtgcagcacc
     1741 tgacggtgga ggtgagcgcg gctgacggtc agtatctggc acaggcgaaa tgggacacgc
     1801 cgcgggtggt gaagggtgtg cgcttcagtc tgcgcctgac cagcggaagc ggtgagaaca
     1861 gccgcctgct gaccaccgcc atcactgccg atacggagca ccgtttcagt ggcctgcctc
     1921 tcggggaata caccctgacg gtcagggcga ttaacagcta tggccagcag ggcgaacccg
     1981 ccaccaccac gttccggatt aacgcaccgg cagcgcctgc cggtgttgaa ctgacgccgg
     2041 ggtattttca gataacggca gtcccgaaac tgaccattta tgacccgacg gtgcagtttg
     2101 agttctggtt ttcggaggca aaaattgcag acgccgcaca ggtggaaacc tctgcccgtt
     2161 atctggggac cggcagtcag tggagtgtct ccggtccgca cattaagccc ggaaaggatt
     2221 tctggtttta tgtgcgcagc gtcaacctgg tggggaaatc tgcttttgtg gaagccagtg
     2281 gccgggccag caatgatgca gaagggtatc tggggctgtt tcgggaaaaa ataggaaaac
     2341 tgcatctggc tcaggggctg tgggagctga tagacaacag ccagcttgcg gatgagatgg
     2401 cggagatgaa gaccagcatc accgaaaccc gcaatgaaat cacacagacg gtcagtaaaa
     2461 cactggagaa ccagagcgcc accatacagc agatacagcg cgtgcagaag gacacaaatg
     2521 atgacctggc tgcgctgtac atgctgaagg ttcaaaaaac gaaagacggc attccctatg
     2581 tggccgggat tggtgcaggg attgaggata ctgatggcca gccactgagc aacatactgc
     2641 tgctggctga ccgtatcgcg atgataaatc cggagagcgg caacagcacg ccgttatttg
     2701 tggcgcaggg gaatcagttg ttcatgaacg atgtgttcct gaagcggctg tttgcggtga
     2761 gtatcacctc gtccggcaat cccccgacgt tttccctgac gccggagggc aggctgaccg
     2821 caagaaatgc tgatatcagc ggtaacgtga atgcgaattc cgggacgctc aacaacgtca
     2881 cgattaatga gaactgtcag attaagggga aactgtcagc caaccagatt gaaggtgata
     2941 ttgtcaaaac ggtcagcaag tctttccccc gcacgaacag ttatgccagt ggcaccatca
     3001 cggtaagaat cagtgatgat cagaa
//
LOCUS       DAZKFZ010000329         2997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000329 DAZKFZ010000000
VERSION     DAZKFZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..308)
                     /locus_tag="ACHOQW_004718"
     CDS             complement(<1..308)
                     /locus_tag="ACHOQW_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9575626.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAEYLHARTSAPG
                     SDNLNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     P"
     gene            complement(376..1098)
                     /locus_tag="ACHOQW_004719"
                     /pseudo
     CDS             complement(376..1098)
                     /locus_tag="ACHOQW_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            1086..1502
                     /locus_tag="ACHOQW_004720"
                     /pseudo
     CDS             1086..1502
                     /locus_tag="ACHOQW_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            1630..2166
                     /gene="yfdR"
                     /locus_tag="ACHOQW_004721"
     CDS             1630..2166
                     /gene="yfdR"
                     /locus_tag="ACHOQW_004721"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9575627.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            2157..2507
                     /locus_tag="ACHOQW_004722"
     CDS             2157..2507
                     /locus_tag="ACHOQW_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFP9575628.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            2504..2977
                     /locus_tag="ACHOQW_004723"
                     /pseudo
     CDS             2504..2977
                     /locus_tag="ACHOQW_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT          777 a          707 c          745 g          768 t
ORIGIN      
        1 ggccccgcca tcacgctgaa ccagcggtta cgcacggaat cttcatgcca gcgggtatcg
       61 ctgtaacggg taagctggcg attcctgtct cctgcatagc tgaatgacgt caccatcccc
      121 agtgtgtccg taaactcata acggtatttc acgttaatcc cgttaagatt atcgctaccg
      181 ggagcgctcg tccgggcatg aaggtactcc gcgctcagcg tggactgatg ttcagacgcc
      241 catgcaggcg cgccggatac ggccagacag atggctgcgg acaaaatggc ggcataaagt
      301 ttacgcataa ttacctctcg cttttctgca ataaaaaagg cgccatttct ggcgcccgta
      361 tatgggttat aaaattcagc tgatactgat gcctgcggtg gctttcttca tcaccacaac
      421 cagcaaatcg ctgatacttg ctgtgggata ccagtcattt accagccatg ctgacaccga
      481 aaactccagc gtcatgtgac cgtgaccggc aggcatatca ataacgccac tgtaaatcag
      541 cgtattatcc agcgcggtac ggttataaat ttcagcaccg tttttccgca ctatcagacg
      601 gcatgaggag taaatatcag tatgctctct ctcatgctta gcgccactga atgccaccgc
      661 cggaataaca atctgccggt caaacggctg atcgtcataa accctgacgg taatggtccc
      721 tgatggccac cgttccggtg cacgggagtc ccgggggaaa gctttgccca ctgttttaac
      781 gagatcgcct tcaatctggt tcgcggacag ttttcccaga acccgacagt tctcgttaat
      841 cgtgacgttg ttgagcgtcc cggagttcgc attcacgtta ccactgatat ccgcattttt
      901 agcagtcagc tttccatccg gtgtcaggga aaatgccggt ggatttccac cactggtaat
      961 ggtgggggcc gtcaggcgct tcaggaacac gtcgttcatg aatatctggt tgccctgcgc
     1021 cacaaacatc ggcgtttcat tcccgtttgc cgggtcaata aacgcgatac gattggcggc
     1081 aaccagaatg gattgaagac tgggccgact accttgtggg ctttgatgct aatggtgaca
     1141 ccattcaggc aacaaaagcg gctgcggcgg tccgtaaaat cacgattgaa gcaaaccaga
     1201 ccgctgattt tgaagataat gacttcagcg gcaaacgctc cctgatggag tctgtcgaag
     1261 cgaagaccaa agacattatg ccagtggcat ttgaatttaa atgcattccg tttgaaggtc
     1321 tgaaagaacg tccgtttaaa ttacgcctca gcattatcac tggcgatcgt ccggtactgg
     1381 ttctgcgcat tattcagctg gaggcggtgc aggaagaaat ggctaacgaa tttcgtgatc
     1441 tgcttgttga gaaattcaag gacagcaaag tagaaacctt tattggtact ttcaccgcct
     1501 gatttcatta ctgcaaatgc ccctgagggg gcatttatgg aaacgtaatt tactcaataa
     1561 tcgccggatg gtgagggatt ctttttacca gaattcagcg cggtgcagcg catatacgtg
     1621 gagaacaaaa tgtcatttat taaaactttt tccgggaagc atttttatta tgacaggata
     1681 aataaagacg acatcgatat taacgatatc gcggtttccc tttcaaatat ctgtcgcttt
     1741 gccggtcatc tttcgcactt ctacagcgtc gcccaacatg cggttctttg cagccagctg
     1801 gtaccgcagg aatttgcttt tgaagcatta atgcatgatg caacagaagc gtattgccag
     1861 gacatccccg ctccactgaa acgccttctt cctgactata aacggatgga agaaaaaatt
     1921 gacgccgtaa tccgtgagaa atacgggtta cccccagtta tgagtacgcc cgtgaaatat
     1981 gccgatctta tcatgctggc aaccgaacgc cgcgatctcg ggcttgatga tggctctttc
     2041 tggcctgtac tggaaggcat cccggcaaca gagatgttca acgtgattcc actggcacct
     2101 agccatgcct acgggatgtt tatggaacgt tttaacgagt tatcggagtt acacaaatgc
     2161 gcatgaatgt tttcgaaatg gaagggtttc ttcgcgggaa atgtgtaccg cgagatctga
     2221 aagtgaatga aacaaatgct gagtatctgg tgcgtaaatt cgatgaagta cgtgctgagg
     2281 ctcgcaacga gggtattaac tataccgcaa gccgtcttgc tgctgctttc aatcacggat
     2341 ttatcaataa gcctttggct gaagttttcg acgttacacg catgattctg tcagcaaaag
     2401 aagagttagc taatgaatcg catccgattg atggcctgtc cggtgaatat gcagagaaat
     2461 cccttgaaga atgggcggaa cggcttcgca aaggaggcag ccagtgactg gacatgcagc
     2521 aatcctcgac atgtgctgtg gcagtcgcat gttctggttc gataagaatg acgaccgggc
     2581 gatatttagc gatatcagaa aggaagagca cacattgtgt gatggacgac gcctgattat
     2641 cagtcctgat ctgatagctg attttcgtgc actaccattt gcagacgcat ctttttcgat
     2701 ggttatattc gaccctccgc atcttgagcg tgttggtgat aacgcctgga tgggaaagaa
     2761 atatggacgg ctgaataaag atacctagcg tgatgatttg cggcagggat ttaaagaagc
     2821 ctttcgtgtg ttgcgtccat ccggcgttct gatttttaaa tggaatgaaa cgcaaatacc
     2881 tgttcgccag atattggtac tgaccgacag aaaacctgtt atcggtcaac gaacaggaaa
     2941 aaacgataaa acccactgga ttatttttat gaaataggca tccagtgagt aggttcg
//
LOCUS       DAZKFZ010000330         2995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000330 DAZKFZ010000000
VERSION     DAZKFZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.332"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            297..1274
                     /locus_tag="ACHOQW_004724"
                     /pseudo
     CDS             297..1274
                     /locus_tag="ACHOQW_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1794..>2995
                     /locus_tag="ACHOQW_004725"
     CDS             1794..>2995
                     /locus_tag="ACHOQW_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFP9575629.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEILEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTFKNIEMAIADIKREYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNP"
BASE COUNT         1010 a          606 c          619 g          760 t
ORIGIN      
        1 gatcacaact gaagatgctt tttacaatga agaattattg ttaaaaaacg ctttgttgaa
       61 tcaaccgaac tgttgctgag ttgcaggatc acatcacgta ttttcctgaa aacgcaggct
      121 gcttagtagc aaagcagaag ttcaaaatca ccaactaaat agctgcgccg aatagtagat
      181 caatgaaagg gaactcagcc cggattgtgc gatctgatca atcgccaaaa atccccccct
      241 aaccggactg agcgatgccg atcatagcac caattccccg cgacgaatga cgcctgatgc
      301 aaaaagcaat ctataaaact cacgataaaa attatgcccg caggctgacc gccatgctga
      361 tgctgcaccg gagcgctcgc gtcagtgacg ttgccagaac gctctgctgc gcccgttcct
      421 ccgtcaggcg ctggattaac tggttcacgc tgtcgagtgt tgaaggtctg aaatcattac
      481 ccgcaggacg ttcccgccgc tggccgtttg agcatatctg cacattgttt cgtgaactga
      541 taaaacactc tcccggatat tttggctatc agcgttcacg ctggagtaca gaactgctgg
      601 caataaaaat caacgagata accgagtgcc agttacatgc cggaaccgtt cgccgctggt
      661 tgccattagt cgggcttgtg tggcgcaggg ctgcgccaac cctgcgtacc cgtaacccgc
      721 ataaagcagc aattcacaaa gcactggacg aatgtagtgc ggagcatccg gtcttttatg
      781 aagatgaagt gtatatctat cttaatccca aaaccggtgc ggactggcaa cttcgcagac
      841 agcaaaaaca tgtggtaaca ccggggcaga atgaaaaata ctatctggct ggtgctttac
      901 atagtggaac gggcaaagtc agctatgtgg gtggcaacag caaaagctca gccttattta
      961 tcagtctgtt gaaacatctg aaattgacat accgacggga taaacctatc acgctcatag
     1021 tggacaacta catcatccat aaaagtcggg aaacacagcg ctggctgaaa gaaaatccga
     1081 agttcagagt gatttatcag ccagtttact cgccgtgggt gaatcatgtt gagcggttat
     1141 agcaggcgct tcacgatacg ataacgcgca atcatcagtg ccgttcaatg tggtaactgc
     1201 ttaaaaatgt tcattatttt atggaaaccg tgagcccatt ccccggtggt aaatatgggc
     1261 tgttaaaagt gtagcggtat tcggcgcagc tatttaggat ggtcaggtcg cagaagttct
     1321 gtgccactac agtgcgtgta ctaaacaaaa tgacgagggc acgaatgtca taatacgtgc
     1381 gtattacctg acagtagcta ccaactgagg gggcttattc caaacctgat ttattcaaca
     1441 aagctggact tatccttact ctgaattatt ttccagacat atgcatcatg tactggctcg
     1501 cttggttcag gaaatacagg agcaaatcta cagctattag atttattcct atttgtatgc
     1561 aaagtcgcat tgcataatgc ggagctatgt tacagatacg cctgacgtgc gtagtaaaat
     1621 tataaagcaa catatatata caaggagaaa ccgagaaaaa taaatgagtc gtatgttggg
     1681 tagcatcgag ataaatattt tttttatcac attagcacct tggttaaggg tgttaataat
     1741 aatattctct cacttattaa aacataaata attttttaag cggaaatgta attatgagac
     1801 tgccagagaa agttcttttt cctcctgtca ctagtggcct gtcagggcag gaaaaacaaa
     1861 aaaaaccgaa gagcattacc ggatttcagg aaaattatca acgcaatatc aggccaatca
     1921 aaacagcatc agaagcccga ctacgcttct ttgataaaat ggtttcgaaa gaaaactctc
     1981 tggaagatgt tgtttcttta ggtgaaatga ttcagaagga aatttatggg catgaacaaa
     2041 gaacattttc accagttcat catacaggta actggaaatc atcattgtta cacaacgcgc
     2101 tccttggtct ggcaaatgtt tataatggct tacgggaaac agaataccct aacactttca
     2161 acagagatgg tataaaaagt actaactctt ttagagataa cttattgaca aaaacaagaa
     2221 ctcccagaga taattttgag gaaggaataa aacatcctga acatgcaaca ataccatatg
     2281 acaacgacaa tgaaagtaat aaattgctaa aagcaggaaa gatagctggt aacaataacg
     2341 agctgttgat ggaaataaaa aaggaatccc aaagcgacca tcaaatcccc ctgtcagata
     2401 agttcctgaa aaggaaaaaa cgatctcctg tagctgaaga taaagttcaa aactcgttaa
     2461 caccagaaaa ttttgttcag aaaatttcac ttagtgatga gcttaaaaca aaatatgcaa
     2521 atgaaattct agagataaaa agaataatgg gagaatacaa tcttttaccg gataaaaaca
     2581 gtcgtaatgg tctaaaactt ctacagaagc aagctgattt actaaaaata atcatggagg
     2641 atacctctgt tacagaaaat acctttaaaa acatagagat ggctatagca gatataaaaa
     2701 gggagtacta ttcgcataca gttgatattg agaaaaatat tcatgccata tgggttgcgg
     2761 gttccccacc tgaaagcatc tcggactata ttaagacttt tctcaaaacc tacaaagagt
     2821 ttacttacta tctttgggtt gatgaaaaag catttggagc cgcgaaattt accagtgtct
     2881 taaaacaaat tgcatttgat ttagcatgca gaactataca gcagaatact ccacaaaaaa
     2941 atattgattt tattaatcta tataatgaaa taaggaagaa atacaacaac aaccc
//
LOCUS       DAZKFZ010000331         2978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000331 DAZKFZ010000000
VERSION     DAZKFZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..333
                     /locus_tag="ACHOQW_004726"
     CDS             <1..333
                     /locus_tag="ACHOQW_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9575630.1"
                     /translation="SPEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSG
                     NNVVIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSVKNNNANGWQLTIIDDH
                     GNYISDKI"
     gene            349..2829
                     /gene="yehB"
                     /locus_tag="ACHOQW_004727"
     CDS             349..2829
                     /gene="yehB"
                     /locus_tag="ACHOQW_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9575631.1"
                     /translation="MLRMTPLASAIVALLLGIEAHAAEETFDTHFMMGGMKGEQVTNL
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPHETCLTREIVKRLGINSDNFAREN
                     QCLTFEQLVQGGSYSWDIGIFRLDLAVPQAWVEELENGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTDNNPGEWKSNALYLEHGF
                     SQILGTLRIGDMYTSADIFDSVRFTGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFSISDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFLSQTSTRFGLAAWRYSSRDYRT
                     FNDYVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMHAPGIKDA
                     YVNGQKYRTTNCNGVVVYDGLTPYRENHLMMDVSQSDSETELRGNRKMTAPYRGAVVL
                     VDFDTDQRKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
     gene            2840..>2978
                     /locus_tag="ACHOQW_004728"
     CDS             2840..>2978
                     /locus_tag="ACHOQW_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFP9575632.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTTASLNITDGINVGEILANET
                     SF"
BASE COUNT          866 a          549 c          741 g          822 t
ORIGIN      
        1 agcccagaag atgaaggaaa gaatgcgtta aaatttgcca tgcaaaacag aatcaagttg
       61 ttttaccgac ctgctggtat tgctccggta aataaaacga cttttaaaaa attacgagtg
      121 agtcgtagcg gtaataacgt agtcataaaa aatgactcgc caaattgggt aacgatttct
      181 gatgttaagg ctaataacgt caaagtgaat tatgaaacaa tcatgattgc accacaagaa
      241 agtcagaatg ttagtgtaaa aaataataat gcgaatggct ggcaactgac cattatcgat
      301 gatcacggca actatattag tgacaaaatt taattgcagg agctgcctat gttgagaatg
      361 accccgcttg catcagcaat agtcgcgtta ttgctcggca ttgaagcaca tgcagcagaa
      421 gaaacctttg acacccattt tatgatggga ggaatgaaag gtgagcaagt aacaaatttg
      481 cgtcttgatg ataatcagcc tttacctggg cagtacgaca ttgatattta tgtcaataaa
      541 caatggcgtg gaaaatatga gattatcgtc aaagataacc cacatgaaac ttgcctgacg
      601 cgtgaaattg taaagcggtt aggtataaat agcgataatt ttgcacgcga aaatcagtgt
      661 ttaacatttg agcaacttgt tcagggcgga agctactcct gggatattgg gatttttcgt
      721 ttggatctcg ctgttccgca agcctgggtt gaagagctgg aaaatggtta cgttccgcca
      781 gaaaactggg agcggggaat caatgcgttt tatacgtctt attacgtaag tcagtattac
      841 agtgactata aagcctcggg caatagtaag agcacttatg tgcgttttaa cagtgggcta
      901 aatttgctgg gatggcagct gcattcagat gccagtttta gcaaaacgga taataatcct
      961 ggtgagtgga aaagcaacgc gctgtatctg gagcatggat tttctcaaat tctcggtacg
     1021 ctgcgtattg gtgatatgta cacctcagcc gatatatttg attccgttcg cttcaccggg
     1081 gttcggttat ttcgtgatat gcagatgttg ccaaactcga agcaaaattt taccccacgg
     1141 gtacagggga ttgcccagag taacgcgttg gtaactattg aacagaacgg ttttgtcgtt
     1201 tatcagaaag aggttccacc aggtccgttt tcgattagtg atttgcagtt agcgggcgga
     1261 ggggcggatc ttgatgttag tgttaaggaa gccgatggtt ctgtgactac gtatctggtg
     1321 ccttatgcgg cagtacctaa tatgctgcaa cctggtgtat caaaatatga ttttgcggca
     1381 ggccgtagtc atattgaagg tgcgagcaag caaagtgatt ttgtccaggc gggttatcag
     1441 tatggtttta ataatttatt gacgctgtat ggcggcacga tggttgctaa taattactat
     1501 gcctttaccc tcggaacagg ttggaacacg cgtattggcg caatttcagt cgatgcaact
     1561 aaatcgcaca gtaagcaaga caatggtgat gtgtttgacg gacaaagtta tcaaattgcc
     1621 tataacaaat ttttgagcca aacatcaaca cgttttggtc tggcggcctg gcgttattcg
     1681 tcgcgtgatt accgcacatt taacgattat gtgtgggcaa ataataaaga taattatcgt
     1741 cgtgataaaa acgatgtcta tgatattgcc gattattacc agaatgattt tggtcgcaaa
     1801 aatagctttt ccgccaatat gagtcagtca ttgccagaag gctggggatc tgtgtcatta
     1861 agtactttat ggcgtgatta ctgggggcga agcggcagta gtaaagatta tcaactgagt
     1921 tattccaata actggcggcg gataagctat accctcgcgg caagccaggc gtatgacgag
     1981 aaccacgccg aagagaaacg ctttaatatt tttatatcga ttccctttga ctggggcgat
     2041 gacgttacga cgccgcgacg gcaaatatat atgtctaact caacgacctt tgatgatcag
     2101 ggttttgctt caaacaatac cggattatcg ggaaccgttg ggaaccgcga tcagtttaac
     2161 tatggtatca acttgagcca tcaacatcag ggaaatgaaa cgacagctgg ggccaatttg
     2221 acctggaccg caccggccgc aacagtgaat ggcagctata gtcagtcgag tacttatcga
     2281 caggtcggag ccagtgtttc agggggactg gttgcctggt ctggtggcgt taatctggcg
     2341 aaccgtcttt ccgaaacgtt tgctgtgatg catgcgccgg gaatcaaaga tgcttatgtc
     2401 aatgggcaaa aatatcgtac aacaaactgt aatggtgtgg tggtgtacga cggactgaca
     2461 ccttatcggg aaaatcacct gatgatggat gtgtcgcaaa gcgatagcga aacagaatta
     2521 cgcgggaacc gtaaaatgac cgccccttat cgcggcgcgg tcgttctggt tgattttgat
     2581 accgatcagc gtaagccctg gtttataaaa gcattaagat ccgatggaca gccattaacg
     2641 tttggttatg aagtcaatga tatgcatggt cataacattg gtgttgtcgg ccagggcagt
     2701 cagatattta ttcgcaccaa tgaaataccg ccagcggtta atgtggcaat tgataagcaa
     2761 caagggcttt catgcacaat caccttcggt aaagagattg atgaaagtaa aaattatatc
     2821 tgtcggtgag aggtgaaata tgatgttcag aaatagaata ttactaatat ttatattgtg
     2881 ggctaatttt acctgggctg ggtgtcgtac tactgcatca ttaaatatta cagatggtat
     2941 taatgttggg gagattttag cgaatgaaac ttccttta
//
LOCUS       DAZKFZ010000332         2952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000332 DAZKFZ010000000
VERSION     DAZKFZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            632..838
                     /locus_tag="ACHOQW_004729"
     CDS             632..838
                     /locus_tag="ACHOQW_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP9575633.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            914..1210
                     /locus_tag="ACHOQW_004730"
     CDS             914..1210
                     /locus_tag="ACHOQW_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9575634.1"
                     /translation="MNAYYIQDCLEAQSWARYYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1216..2001
                     /gene="bet"
                     /locus_tag="ACHOQW_004731"
     CDS             1216..2001
                     /gene="bet"
                     /locus_tag="ACHOQW_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9575635.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1998..2675
                     /locus_tag="ACHOQW_004732"
     CDS             1998..2675
                     /locus_tag="ACHOQW_004732"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9575636.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2675..2857
                     /locus_tag="ACHOQW_004733"
     CDS             2675..2857
                     /locus_tag="ACHOQW_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9575637.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2830..>2952
                     /locus_tag="ACHOQW_004734"
     CDS             2830..>2952
                     /locus_tag="ACHOQW_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9575638.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLA"
BASE COUNT          813 a          725 c          727 g          687 t
ORIGIN      
        1 gcctacgact tgttgattca tggaaatgtt gaaaagataa attaaataaa cgccccgtac
       61 aaagcgatgg cggggctaat aagtcattta tgtatcattt agcaactgac taaaaaaata
      121 cacataagaa ctcagactac caatattcga ttaaatttgc tgccaaccta ccgagttcac
      181 cacaacccga tgcccctctg tcacgaggca cattaaagct gtacaaattt cagccacacc
      241 aataagtctt catactcttt tttataaaaa ttcatttaat tatcaatcag aacaagtata
      301 tcaaacatta tcaatactaa ttgctattga ccacacaata gccattgcta ttgtcatgct
      361 attgcatcga tgttggtatc acaaaattta taacttcacc gttgcgatga ccgcttagat
      421 ccgcagcttg aatttcagca ggctccgggg agtgcgaggg gtgaagcgga cgcgtgaacg
      481 tcggtgtgac cagctgaaat caactcaaca cttcatacct cagtcgcttc aacgaggcga
      541 cttagttatg acaaccggcg gccatccacc gcctgaatac gcgcagaagt ctctatatgt
      601 tcagcagccc agcttacggg caggagtttt tatggttcat caacattacg gaacgcagac
      661 cgttaatcga ggtgcggtca tgccaggaat gctggtcaaa cacaaagatg gtacctggac
      721 tgcatcagct aatttacgcg gacggcttta tctgcatcgc ggcatcgagc gcacttatac
      781 ccgtgatttg ctcgtggaag tttttctcga cggacgcggt aacggcctga atcgctaatc
      841 ccctttcctg ttttcctaat cagcctggca tttcgcgggc gatattttca cagccatttt
      901 caggagttca gccatgaacg cttattacat tcaggattgt cttgaggctc agagctgggc
      961 gcgttactac cagcagatcg cccgtgaaga gaaagaggca gaactggcag acgacatgga
     1021 aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc gatcatttgc aacgccacgg
     1081 ggccagcaaa aaagccatta cccgtgcgtt tgatgacgat gttgagtttc aggagcgcat
     1141 ggcagaacac atccggtaca tggttgaaac cattgctcac catcaggttg atattgattc
     1201 agaggtataa aacggatgag tacagcactc gcaacgctgg ctgggaagct ggctgaacgt
     1261 gtcggcatgg attctgtcga cccacaggaa ctgatcacca ctcttcgcca gacggcattt
     1321 aaaggcgatg ccagcgatgc gcagttcatc gcattgttga tcgtcgccaa ccagtacggc
     1381 cttaatccgt ggacgaaaga aatttacgcc ttccctgata agcagaacgg catcgttccg
     1441 gtggtgggcg ttgatggctg gtcccgcatc atcaacgaaa accagcagtt tgatggcatg
     1501 gactttgagc aggacaatga atcctgcaca tgccggattt accgcaagga ccgtaatcat
     1561 ccgatctgcg ttaccgagtg gatggatgaa tgccgccgcg aaccattcaa aacccgcgaa
     1621 ggcagagaaa tcacggggcc gtggcagtcg catcccaaac ggatgttacg tcataaagcc
     1681 atgattcagt gtgcccgtct cgccttcgga tttgctggta tctatgacaa ggatgaagcc
     1741 gagcgcattg tcgaaaatac cgcatacact gcagaacgtc agccggaacg cgacatcact
     1801 ccggttaacg atgaaaccat gcaggaaatt aacactctgc tgatcgccct ggataaaaca
     1861 tgggatgacg acttattgcc gctctgttcc cagatatttc gccgcgacat tcgtgcatcg
     1921 tcagaactga cacaggccga agcagtaaaa gctcttggat tcctgaaaca aaaagccact
     1981 gaacagaagg tggcagcatg acaccggaca ttatcctgca gcgtaccggg atcgacgtga
     2041 gagctgtcga acagggagat gatgcgtggc acaaattacg gctcggcgtc atcacagctt
     2101 cagaaattca caacgtaata gcaaaacccc gatcaggaaa gaagtggcct gacatgaaaa
     2161 tgtcctactt ccacaccctg ctggctgagg tttgcaccgg tgtggctccg gaagttaacg
     2221 ctaaggctct ggcctgggga aaacagtacg agaacgacgc cagaaccctc tttgagttca
     2281 cttccggcgt gaatgttact gaatccccga tcatctatcg cgacgaaagt atgcgcaccg
     2341 cctgctctcc cgatggttta tgcagtgacg gcaacggcct tgagctgaaa tgcccgttta
     2401 cctcccggga tttcatgaag ttccggctcg gtggtttcga ggccataaaa tcggcttaca
     2461 tggcccaggt gcagtacagc atgtgggtga cacgaaaaga tgcctggtac tttgccaact
     2521 atgacccgcg catgaagcgt gaaggcctgc attatgtcgt gattgagcgg aatgaaaagt
     2581 acatggcgag ttttgacgag atggtgccgg agttcatcga aaaaatggac gaggcactgg
     2641 ctgaaattgg ttttgtatat ggggagcaat ggtaatgaag catcctcacg ataatatccg
     2701 ggtaggcacg atcactttcg tctactccgt tacaaagcga ggctgggtat ttcccggcct
     2761 ttctgttatc cgaaatccac tgaaagcaca gcggctggct gaagagataa ataataaacg
     2821 aggggctgta tgcacaaagc atctcctgtt gaattaagaa cgagtatcgg gatggcacat
     2881 agcctcgctc aaattggagt caggtttgtg ccaataccag tagaaacaga cgaagaattt
     2941 catacgttag cc
//
LOCUS       DAZKFZ010000333         2914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000333 DAZKFZ010000000
VERSION     DAZKFZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.335"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            33..1238
                     /gene="yedE"
                     /locus_tag="ACHOQW_004735"
     CDS             33..1238
                     /gene="yedE"
                     /locus_tag="ACHOQW_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP9575639.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1235..1468
                     /gene="yedF"
                     /locus_tag="ACHOQW_004736"
     CDS             1235..1468
                     /gene="yedF"
                     /locus_tag="ACHOQW_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP9575640.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1577..2245
                     /locus_tag="ACHOQW_004737"
     CDS             1577..2245
                     /locus_tag="ACHOQW_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP9575641.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            2356..2835
                     /locus_tag="ACHOQW_004738"
     CDS             2356..2835
                     /locus_tag="ACHOQW_004738"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9575642.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT          655 a          724 c          770 g          765 t
ORIGIN      
        1 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
       61 ggttgattaa attctgggcg cccatccccg cggtcatcgc ggcgggtatt ctctccactt
      121 actattttgg catcactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
      181 gtcagctcct gcagctgttc ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
      241 atctggaagg atcgccatta acccgcatcg acgggatgat gatcctcggt atgtttggcg
      301 gctgctttgc cgcagcgctg tgggccaaca atgtcaaact acgcatgccg cgtagccgta
      361 tccgcattat gcaggccatt attggcggca ttatcgccgg ttttggcgcg cgtctggcaa
      421 tgggctgtaa cctggcggca ttctttaccg gtattcctca gttctcgctg catgcctggt
      481 tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca
      541 tcttccgtat tcccgtgaaa atgcagaaag ttgctgccgc ctcaccgctg acgcaaaaac
      601 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct
      661 gggcgctgct cacagcgatg aaccaaccca aactggggct ggcaatgctg tttggcgtcg
      721 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
      781 ggatcaccgg acgtacccat atggcgaaag caatcattat tggtatggcg gtgagtgcca
      841 tcgggatctt cagttacgta cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa
      901 acgcggtaat tggtggttta ctgtttggtt ttggcatcgt gctggctggc ggctgcgaaa
      961 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
     1021 atgtgatcgg ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
     1081 ccgactggga caaaatcaac ttgctgaaaa cctttggtcc gatggggggc ctgctggtga
     1141 catatttgct gttgtttgct gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
     1201 gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
     1261 ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc
     1321 gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
     1381 tattccactg gatgcgcgta atcacggcta tacggtgctg gatattcagc aagacgggcc
     1441 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtgaccc acagcgggaa
     1501 catcgttacc tttgtatcac atagatttta cccgccccct ctcctattct tttcagtatg
     1561 atctcaggag gtttctatgt gtggacgctt tgcccaatcc caaacgcgtg aagattacct
     1621 tgcgcttctc gcggaagata ttgaacgcga tattccctat gatcccgaac ccattggcag
     1681 atacaacgtc gcgccgggaa ccaaagtcct actgctcagt gaacgtgatg aacaccttca
     1741 tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa
     1801 cgcccgcgta gaaactgcgg caaccagtcg tatgtttaaa ccgctctggc aacatggtcg
     1861 ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaagcagcc
     1921 ttattttatc tatcgtgctg atggacaacc tgtttttatg gcagcgatag gcagcacacc
     1981 gttcgagcgc ggtgacgaag ccgaaggatt tttgattgtc actgcagcag cagaccaggg
     2041 tctggtagat attcatgacc gccgcccgct ggttctgtcg ccagaagctg cgcgggaatg
     2101 gatgcggcaa gagattagcg gtaaagaagc ctcagaaatc gcggccagtg gctgtgttcc
     2161 agcaaaccag ttctcctggc acccggtatc gcgcgcggtg ggtaatgtta aaaaccaggg
     2221 tgcggagtta attcaacctg tttgataagc ctggagatta ttgatcaaca atactgcgtc
     2281 ataagtaatc tctattagac aaagatttca ttacctgttg gcatattgca aaaataacac
     2341 caatacggaa tcgtcatgtt cacgattaaa acagatgatc tcacccatcc agcagtgcaa
     2401 gcattagtgg cttaccatat ttccggcatg ctgcagcagt ctccccctga aagcagtcat
     2461 gctttagacg tgcaaaaatt acgtaacccg acagtgacat tctggtcagt atgggaaggc
     2521 gaacaactcg caggaattgg tgcgctgaag ttgctggatg ataaacatgg cgaactgaaa
     2581 tcaatgcgga ccgcgccaaa ttatttacgt cggggtgtcg ccagtctgat tttacgccac
     2641 attttgcagg tcgcccatga cagatgcctt catcgcctga gtttagaaac gggtacacag
     2701 gctggattta cggcctgcca tcaactttat ttgaagcatg gtttcgttga ttgcgaaccg
     2761 tttgccgatt atcaacttga tccacacagt cgatttttgt cattgacgct atgcgaagat
     2821 aatgagttgc tttgagccag acgcagcaca ttcttgcatt cgacgtgctg cgtctttatt
     2881 tatcaccaac aggaaacgcc ttgtccatag acgc
//
LOCUS       DAZKFZ010000334         2890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000334 DAZKFZ010000000
VERSION     DAZKFZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.336"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            290..1015
                     /locus_tag="ACHOQW_004739"
     CDS             290..1015
                     /locus_tag="ACHOQW_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575643.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            973..1650
                     /gene="bglJ"
                     /locus_tag="ACHOQW_004740"
     CDS             973..1650
                     /gene="bglJ"
                     /locus_tag="ACHOQW_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP9575644.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1688..2476)
                     /gene="fhuF"
                     /locus_tag="ACHOQW_004741"
     CDS             complement(1688..2476)
                     /gene="fhuF"
                     /locus_tag="ACHOQW_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP9575645.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2617..2853
                     /locus_tag="ACHOQW_004742"
     CDS             2617..2853
                     /locus_tag="ACHOQW_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP9575646.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          778 a          604 c          711 g          797 t
ORIGIN      
        1 atatcattta attataaatt aaatgaatga gatttctgtt tttcattaag tatttctaac
       61 aacttaatgt agatatttta aatgtctcca ggctatttca ccaggttatc atttgatgct
      121 aaccttctga cacatgcagt ggagttgttg tgcagcagga gtatgctgat atgaaagtga
      181 atgctaagga taatttattc gcttaatcta ttaatttgct ggaaacattt aaggaatgaa
      241 tatcctgaat aaaccatcat aatttttaga cagtggatgt ggaggaaata tgttgccagg
      301 atgctgcaaa aatggaattg ttatcagtaa aatacctgtt atgcaagcag ggttaaaaga
      361 ggtcatgagg actcacttcc ctgaatatga aataatatcc agcgcctctg cggaggacct
      421 taccttatta caattacgtc gttccggatt agtcattgct gatttagccg gtgaaagtga
      481 agatccacgt tctgtttgtg aacattatta ttctttaatc tcacaatatc gggaaattca
      541 ctgggttttc atggtgtcac gctcttggta ttcccaggct gtagaactgc tcatgtgccc
      601 tacggcgacg ttattgtctg atgttgaacc cattgagaat ctggtcaaga ccgtacgttc
      661 cggcaatacg cacgcagagc gtatcagcgc catgctgacc tctccggcaa tgactgaaac
      721 tcatgatttt agctatcgct ccgtcattct cactctttca gagcgcaagg tactgcggct
      781 attaggtaaa ggatggggca tcaaccagat agcttcattg cttaagaaaa gtaataaaac
      841 tatcagcgcc caaaaaaaca gtgcgatgcg tcgactggca attcacagca acgctgaaat
      901 gtatgcatgg ataaatagcg cgcagggtgc aagagaactt aacttgcctt ctgtttatgg
      961 agatgccgca gaatggaaca cagccgaatt aagaagagaa atgtcgcact catagaaaaa
     1021 tgcgtcatga gtagtatcgg tattgagagt ttattcagaa agtttgcggg caacccttat
     1081 aagctccata cctataccag tcaggagtca tttcaggatg ccatgtcgcg gatctcgttt
     1141 gcggcggtca ttttttcttt ttctgccatg agaagtgagc gcagagaggg attatcttgc
     1201 ctgactgaac tggcgattaa gtttccgcgt acccggcgtt tagttattgc ggatgatgat
     1261 attgaagctc ggctgattgg ttcattgtcg ccatcaccgc tggacggtgt attaagtaaa
     1321 gcgtcaacgc tggagatttt tcatcaggaa ctctttttgt cattaaatgg tgtacgtcag
     1381 gcgaccgacc gactgaacaa tcagtggtac attaaccaaa gccggacgtt aagcccgacg
     1441 gagagagaaa tattgcgctt tatgtcgcgt ggctactcaa tgacacaaat tgccgagcag
     1501 cttaaacgca atatcaaaac gatccgtgca cataaattta atgtgatgtc gaaactgggc
     1561 gtcagttctg acgcagggtt gttggaggcc gcagatattc tgttatgtat gcggcattgc
     1621 gaaacaagta atgtgttgca tccctattaa tccgcatgat gccgggttta cttccccggc
     1681 agtgctttca tttcagcgta caatcgccac attgctgtac atccggtaag cgataacgct
     1741 ggcagcaagt gcggcgcacc agcaggccgt cgcgtagcac cacggtacgc cagagtggat
     1801 tatcttcacc ggtagtaagc gttttctcaa aaaagagggc atggcgcagc gattcaaccg
     1861 tcgcctcgcc gagcagttgc ttcatctcag taagatacca gttgatcaaa taaccggtat
     1921 tactccagat aagtttgccg ttgatctctc cggtcgcttc tagtgcctgc acaaccggaa
     1981 ccagcgcctg gctgattaac gtttccattc gctgctgcgg cgaatgtggt gttgcgtttt
     2041 tatcttcaca cacatcgacc cagaaacagg cgacgcgtcc ggtttcgtga aactcagcat
     2101 ggaaatgttc cggcgacaca tctaatgctt tttcctgcgt cagtagcgcc agcattaatg
     2161 gtggcaccat caggccgata taccattgtg cccatagtga gatcagcggt ttgtactcgc
     2221 ggatcatcgt tggttggttg cgatagatat gatcggaata gaccgccagt agagaactta
     2281 gcgcattcgg tgatgaccat tgcgccagcg tcatggcgtt aagcggagcg ggttcatcca
     2341 ggcggataaa ctccagcaaa tgttcacgat gttcggcgat cgtcgcacgt acggcttgtg
     2401 caagcctagc atcctgtggc tggagatgcg ttcgccagat gatatcttca tagagcggtg
     2461 cggaacgata ggccataatc gggatagtaa tctaaatgat aatgattgct aatcctagcg
     2521 ataggtttac ccgatagcaa gggatttctc tggcttgcaa gtgataaaaa ttatcatatg
     2581 atattggtta tcattatcaa tgaaagagat aaaaccatgt tgcaacgtac gctgggcagt
     2641 ggctggggag tgttgctgcc gggatttctg attgcagggc tgatgtatgc ggatttatcg
     2701 ccggatcagt ggcggattgt cattctgatg ggattagtat tgacgccggt gatgctgtat
     2761 cacaaacagt tgcggcatta cattttgcta ccatcgtgcc tggcgcttat tgctggcatc
     2821 atgctgatga taatgaattt gaatcaggga tgaaaaaaca gggaagaaac aagaaaggaa
     2881 gtaaagataa
//
LOCUS       DAZKFZ010000335         2869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000335 DAZKFZ010000000
VERSION     DAZKFZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..99
                     /locus_tag="ACHOQW_004743"
     CDS             <1..99
                     /locus_tag="ACHOQW_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9575647.1"
                     /translation="RRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            187..867
                     /locus_tag="ACHOQW_004744"
     CDS             187..867
                     /locus_tag="ACHOQW_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9575648.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            864..1028
                     /locus_tag="ACHOQW_004745"
     CDS             864..1028
                     /locus_tag="ACHOQW_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575649.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            1033..1566
                     /locus_tag="ACHOQW_004746"
                     /pseudo
     CDS             1033..1566
                     /locus_tag="ACHOQW_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            1727..2485
                     /gene="paa"
                     /locus_tag="ACHOQW_004747"
     CDS             1727..2485
                     /gene="paa"
                     /locus_tag="ACHOQW_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFP9575650.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
BASE COUNT          843 a          591 c          750 g          685 t
ORIGIN      
        1 cggcgacaac tggcggcccc caaacgcccg tcggggccaa cgccaattga gttgctgaaa
       61 caggaatatg aacgccggaa agcggctggt tttgtttgag ttgagaagtg attttttacc
      121 gggaggaaat ttatggagac tgtttttgac gcactgaaag cgatgggaaa agccacgtcg
      181 gtatagctgg ctgcgcgact tgatatcagt cgtgaagaag tactgaacga gctgtgggaa
      241 ctgaaaaagg ctggcttcgt tgataaaagc gtatacacct ggcgtgtggc tgataacaac
      301 gttcagcagg aacagccagc gccagaagaa accaccacgg caacagaagc gaaaatctca
      361 gagtgcgatt taaccgcgac gattgaacaa cgcggaccac aaacggcgga tgaactggct
      421 acgctgttcg gtacaacatc ccgcaaagtt gcttcaacgc tggcaatggc aatcagcaaa
      481 ggtcgtctga ttcgcgtaaa ccagaacggt aaaattcgtt actgcatacc aggtgataat
      541 ttaccagcag agccgaaagt tgaatcggta gcggaaaccg atggtaaagc ctttcctcag
      601 ccagccggtg ttgcgttacc ggtacagaaa gatgcaacac aggaagatat taaaacagaa
      661 actgtggcgg acattgtgca gtcgctgcca tcgtttactg caacgcgaga agatgatttg
      721 attttgccat cgctgcatat ggcaaatcgc gaactgcgta gggcgaagaa tcatgtccag
      781 aagtgggagc gagtctgcgc cgcgctgcgg gagctgaaca agcaccggga tatggttgcc
      841 gggatttgtc ggaagtccgg gcaatgagcg gatggtgcag gcctgaaatc atgatactaa
      901 caatgaaggt aaaatgcatc ggcagtctga ttggtcgtag tgaggcggcg gtcaggatga
      961 aagcccaggt taagggaata agcctgattc tgcggggtga ttttcaccag tcaacaaaat
     1021 atccgtagcg cgataacggt caaaaattat ggcgctgaca cttttgtgcc actggagatg
     1081 actgtaccta agttcagggg agaagaacac gtccggtggg atggtcgggc cagatttaaa
     1141 gggcaggtca tggctccagc ctgtacgctg gcaatggagg ctgcctggcg ggaaattgat
     1201 atgggaacca cgccactcag ggatttactg ccggtccaga gaataaattc ctgttacggt
     1261 tacaccactg tgatcttgca agtgcaggaa agtaggtcta cacggtaacg cgagtgcgtg
     1321 taacttttga tgtcattccc gtagaaacac cggacaaatt ttcgctgaca ggtcatgcag
     1381 aaggtataaa tctgcagatt atggacaatt acggatatcc ggcaagagcc ggaaaaagca
     1441 tgccgcctct aattctcagt ggaagatgga cttgattata ctcattgcat tgtcagaaat
     1501 agttatccat taaaggctgg ctattccaaa caggatgttg attacaaaaa tgtaatcaac
     1561 atgtaaggtt tatactcttc aatatgcgta taattttcct tattttgttg actttaaata
     1621 acaagctatg cacgaggtaa agtcggataa gtttatctgg atgtaatata tattatttgt
     1681 agtgtttata actttatttc atgataacca ataaaaggag ttttttatga ggaacataat
     1741 ggcaggtttt ttaatattcc tgtcttctgc tgcttatgct gatatcaatc tgtatggtcc
     1801 tggtggcccg catacagcct tgcttgatgc agccaaactt tatgccgaaa aaacaggtat
     1861 tatagtgaac gttcattacg gcccacagaa caaatggaat gaagatgcca aaaaaaatgc
     1921 agatatcttg tttggcgcat cagaacaatc tgctctggct atcattcggg accataaaga
     1981 cagcttcagt gaaaaagata ttcagcctct ttatctgcga aaaagtattt tactggtaaa
     2041 gaaaggtaat cctaaaaata tccggagtat tgacgacctg accagacctg ggattggcgt
     2101 aattgttaat gatggtggtg gtaccagtaa tacatcaggc actggcgtct gggaagatat
     2161 tgccggacgt aaagggaata tagaaactgt cgccgcaatc cgaaaaaata ttattttata
     2221 tgcgcccaat agcggaactg cacgtaaggc tcttgagaat cagcctggag cagatgtctg
     2281 gataacctgg gctgactggg cagccagtaa tccagaaatt ggtgatgtcg tggaaatagc
     2341 gccagactac gtgatatggc gtgatatgaa cattacagta cgtcaggatg caaatgatga
     2401 aacccgtcga tttgcagaat ggctacaaac cgatgaagcg gcgcctgcat tcaaaaaata
     2461 tggctggacc aggaaaggca cttgacatcc tcgtccttca ggacgtggat tctttttccg
     2521 gatgccgcgc cagcggcatg taggggcagc tcacaaaacg gaaaaaattg tacgctaagc
     2581 ctcgccaggt gaactgaatt cattccgata tgggaattcc catatcgggc gaaaacggtt
     2641 tgctgtaacg gcagagttaa gtaggattgc tgcgggtgct tgaggctatc tgcctcgggc
     2701 atgaacacca acggcagata gataaaagcc ccacccgact ataaatcgaa gtgaggcccc
     2761 tatatgctcg tcacatatag attgcctctt acggaccgaa aggtcaagga gaagcaggct
     2821 atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaa
//
LOCUS       DAZKFZ010000336         2856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000336 DAZKFZ010000000
VERSION     DAZKFZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            72..704
                     /gene="comR"
                     /locus_tag="ACHOQW_004748"
     CDS             72..704
                     /gene="comR"
                     /locus_tag="ACHOQW_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP9575651.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(766..1305)
                     /gene="ycfJ"
                     /locus_tag="ACHOQW_004749"
     CDS             complement(766..1305)
                     /gene="ycfJ"
                     /locus_tag="ACHOQW_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9575652.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="ACHOQW_004750"
     CDS             complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="ACHOQW_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP9575653.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          687 a          774 c          704 g          691 t
ORIGIN      
        1 ctggactggt gaacggaggt gattattttt ataataacct ttcattcaca gtcaccaggt
       61 acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgt ggccgcccaa
      121 aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg caacacggtt
      181 atgaagcgac ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat
      241 acgcggaatt taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc tatatcgatc
      301 gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc
      361 tggctgacta ttttgctgcc atcgccaact gctttaccgg caaagacacc ccggcaggct
      421 gcttcatgat caacaactgc accaccctct ccccagattc aggagatatc gccaatacgt
      481 tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt caacgacaag
      541 cgcgcgggga aatcccgacc cactgtgacg tgacacatct ggcagaattc cttaattgta
      601 ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gttggaaaaa ctgatgcaga
      661 ttgccggaac gactttgcgt ttatggcccg aactggtgaa ataagtcgca aaaaaagccc
      721 ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt
      781 caaaatcagt tgcccgttac tatctagcgg gatctgcgta cccggatcgc ggtccatgcg
      841 gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga
      901 cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag
      961 agagccctgg atctggttac cggcatatcc accacccagc gcccccacaa cagtggcgac
     1021 atctttacca cgaccaccac caaactgatg ccctatcacg ccgccagcaa cagcgccgag
     1081 caccgacccg gtaatgcgat gttcatcctg caccggtcga cgatgggtca ctgtgacgtt
     1141 gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg
     1201 agcgtattgc gggccacgtt caaacacgtt cagactggcc actgccgcta cgccaagcgc
     1261 agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa tacctccttc
     1321 tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa
     1381 aagcgcggga atggctggaa atacttaagg attcggagga gtctgaagga atgcctgatg
     1441 cgacgcttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggatgagg
     1501 cgtttatgcc acatccgcca gtgtacgtcg attaatgcaa cttcaaacgc ggacggataa
     1561 cgcggttaat actccccacc agcatcatta atccggtttt aaagtaacca tgcagcgcaa
     1621 tctgatgcat tcggtatagc gagatatata caaagcgcgc aattcgtcct tcaatcatca
     1681 ttgagccgcg cgtcaggtta cccatcaggc taccaacggt ggagaagttc gacagcgata
     1741 ccagcgaacc atgatcttta tactgataat ttttcagcag cttaccgttc atctgcgcca
     1801 gaatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc ggcggaacaa
     1861 agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtaaatg tctggatcgc
     1921 gggtggtttg cagcgtcagt tccaccacca gctggttgat acggttagtt tcaagaccac
     1981 cgatatcttt caggaagtct ggcgctttga tcccggctgc ccataccatc agatcagcct
     2041 caatatattc gccatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt
     2101 gcgtcagaac gcgaacgcca agtttcgtta gctcgttgtg ggccgcagca gagatacgtg
     2161 gcggtaatgc aggcaaaata cgttctcccg cttctaccag cgttacgttc agggcttcgt
     2221 tggtcaggcc tttgtaaccg tagctgtgca gttgcttgac cgcgttgtgc aattcagcgg
     2281 agagttctac acccgtcgcg ccgccgccga caatcgcaat gttcactttg ccatttgcgc
     2341 ccaggttggc ggagtatttc aggaagagat tcagcatctc ctggtgaaag cgacgcgcct
     2401 ggtgcgggtt atcgaggaaa atgcagttct ctttgacacc tggtgtattg aaatcgttag
     2461 aggtgctacc cagcgccatt accagggtgt cataggcgat tttacgttcc ggaaccagca
     2521 gttcaccttt ctcatcgcgc agttctgcaa tagtgattgt tttcgcgtca cgatcaatat
     2581 caatgacgga acccagctgg aactggaaac catgattgcg cgcatgggcc agatagctca
     2641 acgcatcgac gccttcatca agcgagccag tcgctacttc gtgcagcagc ggtttccaca
     2701 ggtggctgtg gttacgatcg accagcgtaa ttttggcttt tttcttgcgt cccagcttat
     2761 gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatttttt
     2821 tcaatggcgt agtcaacgtg acccccttaa atttat
//
LOCUS       DAZKFZ010000337         2854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000337 DAZKFZ010000000
VERSION     DAZKFZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHOQW_004751"
     CDS             complement(<1..203)
                     /locus_tag="ACHOQW_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9575654.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..954)
                     /locus_tag="ACHOQW_004752"
     CDS             complement(355..954)
                     /locus_tag="ACHOQW_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9575655.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFYGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1022..>2854)
                     /locus_tag="ACHOQW_004753"
     CDS             complement(1022..>2854)
                     /locus_tag="ACHOQW_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9575656.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGV
                     RFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTF
                     RIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETAARYLG
                     SALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKT
                     HLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDT
                     NNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTK
                     PMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSG
                     TLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQP
                     FDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMP
                     AGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT          645 a          836 c          686 g          687 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgaccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatggtca cggattcggt cgggttaaac tgcacgccag ccccccacgc
      481 cagagacgtg ttgctgtggc gaccgtcatc acttccggtc agcacatcgt gcgttttccc
      541 cttgttgtca gttacgcgga gataatcccc gtagaaagtc gacacacggc tgtaagccac
      601 acccgccatc gcatacgcgc tgaaccattc attcacgcgt acagacggcc ccgccatcac
      661 gctgaaccag cggttacgca cggaatcttc atgccagcgg gtatcgctgt agcgcgtttt
      721 ttgctcatcc tcagcattgg cataactgaa ggacgtaatc agccccagcg cgtccataaa
      781 ctcataacgg tatttcacgt taatcccgtt cagatcatca ctaccgggaa cgttcgtcga
      841 ggcatggaga taccccgcgc tcagcgtgga ctgatgttct gctgcactcg ctggcgtagc
      901 agcggcgacc tgccagacta ctgcggacaa aataacagca cataatttac gcataattac
      961 ctctcgcttt tctgcaataa aaaaggcgcc atttctggcg cccgtatctg ggttataaaa
     1021 ttcagctaat cgtgatgcct gcagtggctt tcttcatcac cacaaccagc aaatcgctga
     1081 tacttgctgt gggataccag ttatttacca gccatgctga caccgaaaac tccagtgtca
     1141 tgtgaccgtg accggcaggc atatcaataa caccactgta aatcagcgta ttatccagcg
     1201 cggtacggtt ataaatttca gcaccgtttt tccgcactat cagacggcat gaggagtaaa
     1261 tatcagtatg ctctttctca tgtttagcgc cgctgaatgc caccgccgga ataacaatct
     1321 gccggtcaaa cggctgatcg tcataaaccc tgacggtaat ggttcctgat ggccaccgct
     1381 ccggtgcacg ggagtcccgg gggaaagctt tgcccactgt tttaacgaga tcgccttcaa
     1441 tctggttcgc ggacaatttt cccagaaccc gacagttctc gttaatcgtg acgttgttga
     1501 gcgtcccgga gttcgcattc acgttaccgc tgatatcggc atttttcgcc gtcagccgcc
     1561 cgtccggtgt cagggaaaat gccggaggat taccgccgct ggtaatggtg ggagccgtca
     1621 gatatttcag gaacacttca ttcataaata tctgatcgcc ctgaccaaca aacatcggct
     1681 ttgtgttgcc attcgcagga ttaatcatcg caatcctgtc tgccgccagc agcacctgac
     1741 tctgcattcc tgctggcgta ttctcaatac cggcaccgat acccgcaata taaaggcgtc
     1801 cgtcctgcat ctgctgcagc ttcactgccc acatgctgtt caggttatta tttgtatcaa
     1861 cctgaacttt ctgtatctgc tggattgccg cactctgatt ttccagtttt ttattgacgg
     1921 tctgcgtgat ttcattgctg acattcgtaa tggacgtcct gatttcagcc aggtccggcg
     1981 caagctgacc gttatcaatc tgcgtccaca gctcctgggc cagatgtgtt ttcccgattt
     2041 ctcctttgaa aaaatccagg taaccttccg catcatcgct cgcccgaccg acagcctcca
     2101 cgaatgccga tttgccaaca gtattcacac tgcggatata aaaataataa tcatggcccg
     2161 gtttgatatt gatactggca gctatccagt acagcgccga gccaagatag cgggctgcgg
     2221 tttcaacctg cctgatatcc gcaatccgct tttccgagaa ccagaactca aactgtaccg
     2281 tcgggtcata aacggcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct
     2341 caatccgcga cggtgctgcc ggtgcggcaa tccggaaggt ggtggtggca ggttcaccct
     2401 gctggccata gctgtttatc gcccgcaccg tcagggtgta ttccccgagc ggcaggccgc
     2461 tgaaacggtg ctccgtgtct gcggtgatgg cggtggtcac cagtctggca tccgttccct
     2521 taccactggt caggcgcaga ctgaagcgca cgcccttcac cacccgcggc gtgtcccatt
     2581 tagcctgcgc cagatactgg ccgtcagctg cactcacctc caccgtcagg tgctgtactg
     2641 ccggtgggat gacgctgttc agggaacctg actgcggctc aaagcgggca ccgttatcca
     2701 cgatggcttc tttttccggt acgtgctgca ccgccgtgat ggcaaaggtg ccgtccgtgt
     2761 tttcccggac ggagacacag cggaacaggc gacggcgcag tgacggcagg gagagtcccc
     2821 acaccccgta tgtctccaca ccatcaggca gggt
//
LOCUS       DAZKFZ010000338         2847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000338 DAZKFZ010000000
VERSION     DAZKFZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            201..1154
                     /gene="ompT"
                     /locus_tag="ACHOQW_004754"
     CDS             201..1154
                     /gene="ompT"
                     /locus_tag="ACHOQW_004754"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9575657.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1341..2825
                     /locus_tag="ACHOQW_004755"
     CDS             1341..2825
                     /locus_tag="ACHOQW_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9575658.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT          940 a          602 c          564 g          741 t
ORIGIN      
        1 cagcaaaaag aaaatttcca gcatattaaa ccacaacaat aatacaacac ttggcaagct
       61 gcccataaag aatgaataaa acgaaacaat aattcaaact taaacaaacc ataaacagtt
      121 aaacaatata caattgactc attaagttag atataaaaaa tacatattca atcattaaaa
      181 cgattgaatg gagacctttt atgcgggcga aacttctggg aatagtcctg acaaccccta
      241 ttgcgatcag ctcttttgct tctaccgaga ctttatcgtt tactcctgac aacataaatg
      301 cggacattag tcttggaact ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag
      361 aaggaggccg aaaagtcagt caactcgact ggaaattcaa taacgctgca attattaaag
      421 gtgcaattaa ttgggatttg atgccccaga tatctatcgg ggctgctggc tggacaactc
      481 tcggcagccg aggtggcaat atggtcgatc aggactggat ggattccagt aaccccggaa
      541 cctggacgga tgaaagtaga caccctgata cacaactcaa ttatgccaac gaatttgatc
      601 tgaatatcaa aggctggctc ctcaacgaac ccaattaccg cctgggactc atggccggat
      661 atcaggaaag ccgttatagc tttacagcca gaggtggttc ctatatctac agttctgagg
      721 agggattcag agatgatatc ggctccttcc cgaatggaga aagagcaatc ggctacaaac
      781 aacgttttaa aatgccctac attggcttga ctggaagtta tcgttatgaa gattttgagc
      841 taggtggcac atttaaatac agcggctggg tggaagcatc tgataacgat gagcactatg
      901 acccaggaaa aagaatcact tatcgcagta aagtcaaaga ccaaaattac tattctgttt
      961 cagtcaatgc aggttattac gtaacaccta acgcaaaagt ttatgttgaa ggcacatgga
     1021 atcgggttac gaataaaaaa ggtaatactt cactttatga tcacaatgat aacacttcag
     1081 actacagcaa aaatggtgca ggcatagaaa actataactt catcactact gctggtctta
     1141 agtacacctt ttaacgaagt taaccagatt ttctccccgg tggtattgta taaccccggg
     1201 gaatttgttt acagttacaa gtagacaata tccactaatt ttgtaagcat ttaacctatg
     1261 tcataacgtg taatctctta ccgcaacaca agacctcaat atattcaaat tttatcttta
     1321 ttccgatatt cagagacaca atgcgaaaaa taattactca tttcaaagtt gttttaacgt
     1381 tacttctacc agtaaccgta tctgcccagc agatacagtg gcaatcctgt atggccagtc
     1441 aattcaacca ctggtttggt gaggaaaaac cgtctcctga cttactatgt ggttatttgt
     1501 ctgttccatt aaaatataca gacacaggcg gagatgcttc ttatgaaaaa aaatcacaag
     1561 tcaaactagc gttgacaaaa ttgccggcaa aaagcaagca taaaggaagt atcctgataa
     1621 taagtggtgg tcccgggtta ccaggcataa atccttatat taactttgac tggccagtca
     1681 caaatcttcg tgagtcatgg gatattattg gatttgatcc tcgaggcgtc gggcagtcca
     1741 ctccgacaat aaactgccgg caatcagata cagagactca ggaaaacata accgaaaagc
     1801 aacaagtatt aaataaaatt aatgcctgta tccataatac cggagccgaa gtcattcgcc
     1861 atatcggctc taacgaggct gtatacgata ttgatcgtat taggcaagcc ttgggggata
     1921 aacaactgac agccgtggcg tattcgtatg gaactcaaat tgcagcctta tatgcagaac
     1981 gttttcccta caacgtaaga tctatcgttc ttgatggagt cgtcgatatc gatgacctgg
     2041 aggacaactt cacatggcaa ctcaaacagg cacagagtta tcaggaaacg tttgatcgct
     2101 ttgcatcctg gtgtgcgcgt acaaaaagtt gcccgctttc ttcagacaga gataaggcaa
     2161 taactcagtt ccatgagcta ttatcaaaat tacatcacaa acctttatta gacagtaagg
     2221 gagaaaatat atcttcagat gaactcatat cattaacaac agaccttctg ctatggcgtt
     2281 catcatggcc aacccttgca actgccatac gccagttctc tcaggggatt gtcagtaatg
     2341 aaattgaaac tgcgctcagt gctccgatag cctcagaaga gtcaagtgat gcttcggggg
     2401 taatcctttg tgtagatcag ggggatgagc aattaacacc agaagagcga aaattccgaa
     2461 aagaggctct tgcgaatgcc ttcccggcta ttaactttga caatggacgt tccgattcac
     2521 ctgatttttg tgaattatgg ccaatacata gcgacctgaa caaaactcgc ctgaaaaata
     2581 ctgttctgcc ctctggttta ctgtttgtag cacacaaata cgacccaaca acgccctgga
     2641 ttaatgcccg taagatggca gagaaatttt ccagcccgtt actaacaata aatggtgatg
     2701 ggcatacatt agctctcacc ggagttaatt tatgtgtaga taaagcagtt gtacatcacc
     2761 tgatcactcc acaaaaaata gaaaatatat actgcccagg aaattctgaa gcagaaatac
     2821 aataatttca aaatattccg ctatttg
//
LOCUS       DAZKFZ010000339         2846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000339 DAZKFZ010000000
VERSION     DAZKFZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            16..492
                     /gene="agaB"
                     /locus_tag="ACHOQW_004756"
     CDS             16..492
                     /gene="agaB"
                     /locus_tag="ACHOQW_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP9575659.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            564..1334
                     /gene="agaC"
                     /locus_tag="ACHOQW_004757"
     CDS             564..1334
                     /gene="agaC"
                     /locus_tag="ACHOQW_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP9575660.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1324..2115
                     /gene="agaD"
                     /locus_tag="ACHOQW_004758"
     CDS             1324..2115
                     /gene="agaD"
                     /locus_tag="ACHOQW_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP9575661.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFSLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2116..2846
                     /gene="agaI"
                     /locus_tag="ACHOQW_004759"
                     /pseudo
     CDS             2116..2846
                     /gene="agaI"
                     /locus_tag="ACHOQW_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT          686 a          674 c          728 g          758 t
ORIGIN      
        1 ggtgaggatt acacaatgag cagtccaaat attctcttaa cccgtattga taaccgtctg
       61 gttcacggtc aggttggcgt gacctggaca tccaccatcg gtgcgaatct gctggtagtc
      121 gtggatgatg ttgtcgctaa cgatgatatt caacagaaat taatgggtat taccgcagaa
      181 acctacggct ttggcattcg tttctttact atcgaaaaaa ccattaacgt catcggcaaa
      241 gctgcaccgc atcagaagat cttcctgatt tgccgtacgc cacaaacggt acgtaaattg
      301 gtagaaggtg gcattgacct gaaagatgtc aacgtcggca atatgcattt ctcggaaggg
      361 aaaaagcaaa tcagcagtaa agtttatgtc gatgaccagg atctcacgga cttacgtttt
      421 attaaacaac gtggcgtgaa tgttttcatt caggacgtcc ctggcgatca aaaagaacaa
      481 atccctgact aaatctaaaa tcgccttaat attggtttga ggtaataaaa atgcatgaaa
      541 taaccctact ttagggatta tccctggcgg cgttagtttt cgttctgggg attgattttt
      601 ggctggaagc cttattttta ttccgcccga taatcgtttg taccctaact ggcgctattc
      661 tcggtgatat tcagactggc ttaattaccg gtggtctgac agagttggct ttcgccggat
      721 taacccctgc aggtggtgtt cagccgccca acccgattat ggcgggtctg atgaccaccg
      781 tcattgcatg gtctacgggc gttgatgcca aaacggcaat tggtcttggc ctgccgttta
      841 gtttgttaat gcagtacgtc attctgttct tctattctgc tttctcatta tttatgacca
      901 aagccgataa atgcgcgaaa gaggcggata cggcagcgtt ttcccgactt aactggacaa
      961 cgatgctcat cgtcgcttca gcgtatgcgg tgattgcttt cctctgtact tacctggcac
     1021 aaggggcgat gcaggcgctg gtgaaagcga tgcccgcttg gctgacccac ggctttgaag
     1081 tggctggcgg tattctgcct gccgttggtt ttggcttgct gctgcgcgtg atgttcaaag
     1141 cacaatatat cccttacctg atcgccggct tcctgtttgt ttgctacatc caggtcagca
     1201 acctgttgcc ggttgccgta ctgggcgcag gctttgcggt gtatgagttt ttcaatgcga
     1261 aatcccggca gcaagcgcaa ccgcagcccg ttgccagtaa aaatgaagaa gaggactaca
     1321 gcaatgggat ctgaaatcag taaaaaagat atcacccgtc tgggctttcg ttcgtcgctg
     1381 ctgcaagcga gctttaacta cgaaaggatg caagcgggcg gttttacctg ggcgatgttg
     1441 ccgatcctga aaaagattta taaggatgac aaaccgggcc taagcgcagc gatgaaagat
     1501 aacctcgaat ttattaatac ccacccgaat ctggtcggat tcctgatggg gttattaatt
     1561 tcgatggaag aaaaaggaga aaaccgcgac accattaaag gcctcaaagt ggcactgttt
     1621 ggcccaatcg ccgggattgg cgatgcgatt ttctggttta ctttgttgcc gattatggcg
     1681 ggaatttgct catcatttgc cagccaggga aacctgctgg ggccgattct atttttcgcc
     1741 gtttacctgc ttatcttttc cctgcgcgtc ggctggaccc acgtcggtta ttcagtcggc
     1801 gtgaaggcga tcgataaagt gcgagagaac tcgcagatga ttgctcgctc ggcaaccatc
     1861 ctcgggatca cggtaatcgg cgggctgatc gcttcgtatg tgcatattaa cgtggtgaca
     1921 tcgtttgcca tcgacagtac ccacagcgtc gcgctgcaac aggatttctt cgataaagtc
     1981 ttcccgaaca ttttaccgat ggcctacacc ctgctgatgt attacttcct gcgggtgaaa
     2041 aaagcgcatc cggtgctgtt aatcggcgtg acttttgtgc tctctattgt ttgttccgca
     2101 ttcggcattt tgtaaatgga acgaggcact gcgtctggcg gtgcctcttt acttaaggaa
     2161 tttcatcctg tgcaaaccct tcagcaagtt gaaaactata cggcgttaag tgaacgtgcc
     2221 agcgaatatt tattggccgt gatccgtagc aaaccggatg ccgtgatttg cctggcgacc
     2281 ggagccacgc cattactgac gtatcattat ctggtagaaa aaatccacta gcagcaggtt
     2341 gatgtcagcc agctcacctt cgtgaagctc gacgaatggg tggatctgcc attaacgatg
     2401 cctggtacct gcgaaacttt cctgcaacag catatcgtgc agccgctggg gctacgtgaa
     2461 gaccagctca tcagctttcg ctccgaagag ataaatgaga cagagtgcga acgggtaacg
     2521 aacctgattg cgcgcaaagg cggtctggat ttatgcgttc tcggattggg gaaaaacggt
     2581 catcttgggc tgaacgaacc gggagaaaac ctgcaaccgg cctgccatat cagtcaactt
     2641 gatgccagaa cacagcaaca tgagatgtta aaaaccgcgg gtcgccccgt gactcgtggg
     2701 atcaccttag gcctgaagga tattctcaat gcccgcgagg ttttgttact ggtgactggc
     2761 gaaggaaagc aggatgcgac agaacgtttt ctcacggcta aagtctctac ctctatcccg
     2821 gcttcgtttt tgtggctgca cagtaa
//
LOCUS       DAZKFZ010000340         2835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000340 DAZKFZ010000000
VERSION     DAZKFZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHOQW_004760"
     CDS             <1..384
                     /locus_tag="ACHOQW_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP9575662.1"
                     /translation="FKNTCFYNVRAKNVYLEGAYLNNDNIVNQANNSTEKQSIDSTDK
                     QANDSTVQQSIDSTVQQANDSTDKQANDNIDKQVNDSTDKQAKNSTEQQDSNSFNQAR
                     LKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            650..1492
                     /locus_tag="ACHOQW_004761"
     CDS             650..1492
                     /locus_tag="ACHOQW_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9575663.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1560..2738
                     /locus_tag="ACHOQW_004762"
     CDS             1560..2738
                     /locus_tag="ACHOQW_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9575664.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
BASE COUNT          736 a          682 c          648 g          769 t
ORIGIN      
        1 tttaaaaata cctgttttta caatgtcaga gcgaaaaatg tctatcttga gggagcatat
       61 ctgaacaatg ataatatcgt gaatcaagcc aataacagta ccgagaaaca atccattgac
      121 agtaccgata aacaggccaa tgacagtacg gtgcaacaat ccattgacag tacggtgcaa
      181 caagccaatg acagtaccga taaacaagcc aatgacaata tcgataaaca ggtcaatgac
      241 agtaccgata aacaagccaa gaacagtacc gagcaacagg acagtaacag ttttaatcaa
      301 gcccgtttaa agaaagaagt gaataggaga ttttccattc cgggtttaac gtcttatcag
      361 ccaacatata tagttgaaga atagttcatt aatcacccaa acagagtggg tatggcagac
      421 gatatagatt aacatttaaa cgttaaccgc ctcatctatc attcatagat gaggcgcatt
      481 tatcagtcga tgtaagctga aaatgttctg ccatccagag acaaccgccg ccccctgcgg
      541 cgtttactat ttcatcgccc gcgttaagaa ttcattaagc ttcatccgta tactccttat
      601 ctgtcgcact gaatttgacg agaaaaaagc aataataagg agcaataagt tgaaaaagcg
      661 catcaccttt atcgtttttc tttgcgccat cgtggcggca agcctgtttt ttgtgcaatc
      721 ctgcgtccgt aaaagccagc atgtcgcagg gtttcagaat tatcaggcca ctatcgatgg
      781 taaagagatt acaggagtaa ctaaaaatat ctcctcactg acctggtcag cgcagagcaa
      841 taccttattt agcactatca acaaacctgc aaccatcgtt gaaatgacga ccgagggcga
      901 cttgatccgc accattccgc tcgattttgt taaggatctc gagacgattg aatacattgg
      961 tgataataaa tttgtcatca gtgatgaacg tgactacgcc atttatgtga tttcacttaa
     1021 cgctgactcg gaagtgagca tcctcaaaaa aattaaaatc ccattgcagg aaacaccaac
     1081 taactgtggc tttgaagggc tggcttactc ctctcaggac cataccttct ggttttttaa
     1141 agagaaaaat ccgatagagg tctacaaagt aaccgggcta ttgcgtagcg atgagttgca
     1201 tatcagcaaa gataaaacgt tgcagcgtca gtttacttta gacgatgttt ctggcgcgga
     1261 atttaaccca cagaaaaata cgttgctggt gttgtcacat gagtcacgcg ccctgcagga
     1321 agtgaccgtg agaggtgacg tgatcggcga aatgtcgtta accaaaggta agtatggcct
     1381 ttcacacaac atcaagcagg cggaagggat agcgatggat gatagcggca acatctatat
     1441 cgttggcgaa ccgaatcttt tttatcgctt tacgtcgaca aaatcacgat aacagtctgg
     1501 caggttctcc ttttttgtta ttaacttgtt aactcaaaat aatacaaacc aggcagaaca
     1561 tgaattcgac atcgcatccc gtagaacgtt tttctttcag caccgcgtta ttcgggatgc
     1621 tggttctgac cttaggtatg ggtttaggcc gctttctcta tacgccgatg ctgccagtca
     1681 tgctggcgga aggcgagttt tcatttagcg aactctcatg gatcgccagt ggtaactatg
     1741 ccgggtatct ggcggggagc ctgctgtttt cattcggcgc gtttcattta ccctcacgcc
     1801 tgcgcccgtt cctgttagct tccgccctcg caaccggatt attaatcctc gcgatggcgt
     1861 ggctgccgcc gtttcttctg gttttcatca ttcgctttct ggcgggagtc gccagcgccg
     1921 ggatgttgat tttcggctca acactcatca tgcaacatac tcgccatccc tttgtccttg
     1981 cggcgctatt ttctggtgtt ggcgtcggca tcgctctggg taatgaatat gtgctggcag
     2041 gcctgcattt tgccctctct tcacaaacgt tgtggcaagg tgccggagca ctttctgcca
     2101 ttatattgct tgctctggcg ctgctcatcc cgtcgaataa acacgttatc ccgccagcgc
     2161 cattggcaaa aatcgcgcaa caacccatga gctggtggtt actggcgatt ctgtatggtc
     2221 tggcgggttt tggttatatc atcgtcgcca cctacctgcc gctcatggcg aaagacgcgg
     2281 gccagcctgt gttgacggct cacctctgga cactggttgg cttgtcgatt gtcccaggtt
     2341 gctttggctg gctgtgggca gccaaacggt ggggagcatt accttgcctg accgcgaatt
     2401 tgctggtgca ggcgatctgc gtgctgttaa ccctcgccag cagctctcct ttattactca
     2461 tcatcagcag tattggtttt ggcggcacct ttatgggaac gacctcgctg gtgatgacca
     2521 tcgcccgcca gcttagcgtg ccgggaaatc ttaacctttt gggctttgtg acactcattt
     2581 atggtatcgg gcaaattctt ggcccggcgc tgaccagtat gctcggcaac ggaacgtcgg
     2641 cgctcgccag cgccacactc tgcggcgcag cggcgctatt tatcgcagca ttaatctgcg
     2701 ggatgcaaat attcaaattg catacgaatt attcttaagt aatttaacta atttaacttt
     2761 tcagcacatc acgtaaaata attaaaatat cctttttctt tggaggtact cagccaccgc
     2821 cggtattatt cctgg
//
LOCUS       DAZKFZ010000341         2820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000341 DAZKFZ010000000
VERSION     DAZKFZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1890
                     /locus_tag="ACHOQW_004763"
     CDS             <1..1890
                     /locus_tag="ACHOQW_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9575665.1"
                     /translation="LAEKLTHKLKEGWQPFGSPVAITPYTLMQAIAAEGDVTTPVVVP
                     GTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTYDRPDPRIKQLARRSTV
                     TPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIP
                     ANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGRPLYKDLIGRTKAALAK
                     NPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLADMVGQCAGGSADGVPWI
                     CGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENGANVPTNKPEEDPDIPA
                     SGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILLHAGRTAELVGGEQVVM
                     PPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWGAEGGRALVVDDAGAAG
                     GKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDGTLTANQYALALYWPVS
                     SLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNTKLGSFGAFDNEWHTLG
                     FRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITSGATYPVLIESITV
                     EVNNP"
     gene            2038..2220
                     /locus_tag="ACHOQW_004764"
     CDS             2038..2220
                     /locus_tag="ACHOQW_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9575666.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2258..2527
                     /locus_tag="ACHOQW_004765"
     CDS             2258..2527
                     /locus_tag="ACHOQW_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9575667.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2603..2818
                     /locus_tag="ACHOQW_004766"
     CDS             2603..2818
                     /locus_tag="ACHOQW_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9575668.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          701 a          661 c          849 g          609 t
ORIGIN      
        1 cttgcggaaa agctgacaca caaactgaaa gagggctggc agccgtttgg tagtcctgtg
       61 gccataaccc cttataccct gatgcaggcg attgccgcgg agggggatgt gaccacgcca
      121 gtggttgtgc ccggcacggg ggatggtggc tatccgggag tggtcaccac ggagccagat
      181 tattactacg ttattccact ggccgggcag tcgaacggca tggcttacgg tgaggggctt
      241 cctctgccgc agacatatga ccgtcctgac ccgcgtataa agcaactggc tcgtcgcagc
      301 acagtgacgc cggatggcgc tccctgtaaa tataacgaca ttattccggc agaccactgt
      361 ctgcatgatg tacaggacat gagccgtctt aaccatccga aagctgacct gtcgaaaggt
      421 cagtacggaa ccgtggggca ggggctgcat attgccaaaa agctgctgcc gtttataccg
      481 gcgaatgcgg gtattcttct ggttccgtgc tgccgtggtg gttcagcttt caccaccggg
      541 gcagatggaa catacagtga cgtgaccggt gcctcagaga gttctacccg ctggggtgtg
      601 ggcaggccgc tgtataagga tctcatcggt cgtacaaaag ccgcgctggc aaagaacccg
      661 aaaaatgtgc tgcttgccgt ggtgtggatg cagggggaat ttgactttga cggaacgcca
      721 gcaaatcaca cagcccgttt tacagaagta gtggaacaat atcgtacgga ccttgcagat
      781 atggtgggac agtgcgctgg tggttctgct gacggtgttc cctggatatg tggagacaca
      841 acttatttct ggaagcagaa gagcgaatcc acttaccaga cggtgtacgg cagttacaaa
      901 aacaaaacgg aaaagaatat tcactttgtg ccgttcatga ccgatgagaa cggagcaaat
      961 gtcccgacga acaaaccgga agaagacccg gatattccgg catcaggata ttacggtgcg
     1021 gcctcccgga cgtcggcaaa ctggacgtca gcagaccgtg cgagccattt cagctcatgg
     1081 gcacgcaggg ggattatttc tgaccgtctt gcctcagcga ttcttctcca tgcaggacgg
     1141 acggctgaac tggtgggtgg ggaacaggtt gtgatgccgc cggatgagaa gccgtcaccg
     1201 gacacaccat caacaccgtc aacggacggg aaatcagtga caacgctgct ttattaccgt
     1261 gcaacagagt caggtggttt actgaatccg cagggatggg gagctgaagg agggcgtgca
     1321 ttggtagttg atgatgcagg tgctgcagga ggtaaggcgc tgaggtggac caaacagaca
     1381 ggaagttcct cgtggtttat gcagcatgat gccggtaatg gcgcagacct gctggagaag
     1441 ggcgggctta tcagttgtcg ttttaaagtt gatggcacac tgacagctaa tcagtacgca
     1501 ctggcgctgt actggccggt ttcttcactg cctcagggcg tcacactgga aggtaatgcc
     1561 ggtcataacc tgctggcgtc gttttacgta cagagcgatg ccacagacct taatgtgatg
     1621 taccacaagg gaaatgctgg tcagaacacg aagctggggt cattcggcgc atttgataac
     1681 gaatggcata cgctgggctt ccgttttgcc ggtaacaaca gtattgaggt gacgccggtc
     1741 attgatggta aggacgggac gccgttcatg ctgtcacagt caccggtcgg cacgtttacg
     1801 gcagacaaat tgcgcgtgac cgatatcact agcggtgcga catatccggt gctgattgaa
     1861 agtataacag tggaagtgaa taacccgtaa gcaggaaaaa aaggccgccg gggcagggaa
     1921 aacaaggagc cagaaccggc ggcaaatgtc gttatatcca aagcaaaaca tgcaggacac
     1981 ttttttaacc aacaggtatt aacgatgtca acaccatatc aataaccggg agggataatg
     2041 agatttgtac agcttatttt attgtatttc tgcacggtgg tgtgcacgtt atatctggta
     2101 agtggcgggt ataaggttat ccggaactat atacgcaaaa agattgatgc cgcggcggcg
     2161 gaaaaaatca gcgccagcca gtcagccgga acaaaacccg aagagcctct catttcgtag
     2221 caactttctt aacaacacct ttcaacgaga aaatcccatg tcagaaataa aatctctggt
     2281 cactgctgaa gcagtgaagg acgtcctgcg ctctgaagaa gtcagaagcg cactgaaaca
     2341 gcaactccgc cagaatcttg aggcgcgtct tgatgctgaa gtggatgcca ttctggatga
     2401 gctgctgggg ggaccggctg ctcctgagcc tgaagacggc gcgggtgaca gtgctgtttc
     2461 agatggcgtt gtgtctcagc ctgacggtag cagtgagcct cagcctggcg gcgaaatgat
     2521 gatgtaacca tacgcagggg ctgtcggtgt gagctgatgc ccctcctgtt gttgtgagct
     2581 tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacgg gtgtgtcata
     2641 caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca aagtctctcc
     2701 gtcccagtgg gtggcgatag gtgtgctggg gagtctgctg tttggcctgc tgacgtatct
     2761 gactaacctg tatttcaaaa tcagagagga ccgtcgtaag gcggcgcggg gagagtaaag
//
LOCUS       DAZKFZ010000342         2811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000342 DAZKFZ010000000
VERSION     DAZKFZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(33..109)
                     /locus_tag="ACHOQW_004767"
     tRNA            complement(33..109)
                     /locus_tag="ACHOQW_004767"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Asp,seq:gtc)
     gene            complement(242..973)
                     /gene="dnaQ"
                     /locus_tag="ACHOQW_004768"
     CDS             complement(242..973)
                     /gene="dnaQ"
                     /locus_tag="ACHOQW_004768"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP9575669.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1038..1505
                     /gene="rnhA"
                     /locus_tag="ACHOQW_004769"
     CDS             1038..1505
                     /gene="rnhA"
                     /locus_tag="ACHOQW_004769"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP9575670.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1502..2224)
                     /locus_tag="ACHOQW_004770"
     CDS             complement(1502..2224)
                     /locus_tag="ACHOQW_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9575671.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2258..>2811
                     /gene="gloB"
                     /locus_tag="ACHOQW_004771"
     CDS             2258..>2811
                     /gene="gloB"
                     /locus_tag="ACHOQW_004771"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP9575672.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLS"
BASE COUNT          726 a          692 c          680 g          713 t
ORIGIN      
        1 ggttctctga actcattttc atgaggtgaa tttggcggaa cggacgggac tcgaacccgc
       61 gaccccctgc gtgacaggca ggtattctaa ccgactgaac taccgctccg cgttgtgttc
      121 cgttgggaac gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc
      181 ttttgaatca tttgctgcaa aaatcgccca agtcgctatt tttagcgcct tttacatgta
      241 tttatgcccg ccagaggcag cttccgccct ttttctgcac cagatcgaga cgggcttcat
      301 gtgctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa
      361 ggcgctggat tgttgcttca ccttgttgct gttgtgtctc tccttccatc gcaaaagcca
      421 tcgacgtttg accaccggtc atcgccagat aaacttccgc aaggatctgg gcatcgagta
      481 atgccccgtg cagcgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga
      541 ggctgttgcg tttaccggga aacattttcc tcgccaccgc aaggctatcg gtgaccttac
      601 agaaggtatt agtcttcgga atatcgcgct taagcaacga gaactcgtag tccataaagc
      661 cgatatcgaa cgctgcgtta tggatcacca actccgcgcc gcggatatag tccatgaact
      721 catcggctac ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta
      781 cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc
      841 ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat
      901 agtgcgcacc aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa
      961 ttgcagtgct catagcggtc atttatgtca gacttgtcgt tttacagttc gattcaatta
     1021 caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg
     1081 gtaatcctgg acctgggggt tacggcgcta ttttacgcta tcgcggacgc gagaaaacct
     1141 ttagcgcagg ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg
     1201 cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc
     1261 gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa accgcagaca
     1321 aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tgctgcattg gggcagcatc
     1381 aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg
     1441 aactggctcg tgccgcggcg atgaatccca cactggaaga tacgggctac caagtcgaag
     1501 tttaagcctg tggtttacga cattgccggg tggctccaac cgcctggcga attcgtggct
     1561 tgtttttact ctgtttcatc ggatttagcg ttaaaggaat agtccgtttc cgggcaacaa
     1621 taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa aagttttcct ccgtgtttgt
     1681 tccacgggag aacgtggaaa cggctggcgt gtagcacttc aaaattcaac aaagagagcc
     1741 agtccagttg ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt ttgcgcaata
     1801 ccggcacaag tttgcgtaat cccattaaac taatgggatt gaagccacta atgaccagcc
     1861 agccatcatc aatcaatacc cgatcggctt cacgcaataa acgatgcgga tcggtgcacc
     1921 acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa
     1981 gtgggtccgc ctgcacctgg acaggcattc cttgcgcaga aacattcact tgatgagaaa
     2041 ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agataaaaac
     2101 catacatttt agtgaaccac gggttgagct ggcgctccag cgccttgcga taaagctctc
     2161 cccagggcaa atcgccccag cgatcaggag ccacgacagt ttgagggact cttgccggtt
     2221 tcatcacaac cttccgtttc acactgagag gtaatctatg aatcttaaca gtattcccgc
     2281 ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga
     2341 tcccggagac gcagagccag tattaaacgc cattgccgcc aataactggc aaccggaggc
     2401 catatttctc acccaccatc atcacgatca cgttggcggc gtaaaagaac tggtggaaaa
     2461 gtttccgcaa attgtggtgt atggtccaca agagacacaa gataagggaa caacacaggt
     2521 agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacgcc
     2581 gggtcacact ttaggacata tctgttactt cagtaaacct tatctatttt gcggcgacac
     2641 gctgttttct ggtgggtgtg gtcggttgtt tgaagggaca gcatcacaaa tgtatcaatc
     2701 acttaaaaag ttaagtgcgt tacctgacga tacattggta tgttgtgctc atgaatatac
     2761 gttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtcca t
//
LOCUS       DAZKFZ010000343         2806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000343 DAZKFZ010000000
VERSION     DAZKFZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..577
                     /locus_tag="ACHOQW_004772"
     CDS             <1..577
                     /locus_tag="ACHOQW_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575673.1"
                     /translation="FSLTSDGKLTAKNADISGSVNANSGTLNNVTVNENCTIKGMLEA
                     TQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAY
                     SDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEF
                     KVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            648..1247
                     /locus_tag="ACHOQW_004773"
     CDS             648..1247
                     /locus_tag="ACHOQW_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9575674.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1307..2623
                     /locus_tag="ACHOQW_004774"
     CDS             1307..2623
                     /locus_tag="ACHOQW_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9575675.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     ATEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGATGPAGP
                     QGPKGETGAAGPVGATGPQGAKGDPGETQIRFRLGPMRIIETNSYGWFPGTDGALITG
                     LTFLDPKDATQVQGMFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            2625..>2806
                     /locus_tag="ACHOQW_004775"
     CDS             2625..>2806
                     /locus_tag="ACHOQW_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9575676.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDA"
BASE COUNT          744 a          634 c          883 g          545 t
ORIGIN      
        1 attttccctg acatcagacg gaaagctgac cgctaaaaat gcggatatca gtggcagtgt
       61 gaatgcgaac tccgggacgc tcaacaacgt cacggtaaat gaaaactgta cgattaaggg
      121 catgctggag gcgactcagg tcagaggtga cttcgttaaa gctgtatcca aatcatttcc
      181 gaaacaggct ggtacgtggg gtaacacgga aacaccaaac gggacggtta cagtcaccat
      241 cagcgatgat cataactttg accgtcaaat cattattccg cccattatct ttaacggaat
      301 agcgtatagc gatccgggaa gtggtaataa cccgggaggt acaagataca cgggttatgg
      361 ttttgaagtt cgcaaaaacg gtgtattaat cgcatccaga gaaactaaag gggccattcc
      421 cggtagctac agtgcggtta ttgatatgcc gagtggcagg ggaagcgtca ctctggagtt
      481 taaggttttc cataaaggca atcagcgggc aggtaatatc accgactgta cggtgattgt
      541 gaccaaaaaa gcggcttccg gcatcagtat ccgttgaaat atttataacc ctaataacgg
      601 gcgccagaaa tggcgccttt tttattgcag aaaatcggga ggtaattatg cgtaaagttt
      661 gtgcagcaat tttgtccgca gccatttgtc tggccgtatc cggtgtgcct gcatgggcgt
      721 ctgagcatca gtccacgctg agcgcaggat atcttcatgc cagtacggac gctcccggca
      781 gcgatgatct gaacgggatt aacgtgaaat accgttatga gtttacggac acactggggc
      841 tgattacgtc cttcagttat gccaacgccg aagatgagca aaaaacgcat tacagcgata
      901 cccgctggca tgaggattac gtgcgtaacc gctggttcag cgtgatggca gggccgtctg
      961 tgcgtgtgaa tgaatggttc agtgcgtatg cgatggcggg tgtggcttac agccgtgtgt
     1021 cgactttttc cggggattat ttccgcgtaa ctgacaacaa gaggaaaacg cacgacgtgc
     1081 tgaccggaag tgatgacgct cgctacagca acacatctct ggcgtggggg gctggcgtgc
     1141 agtttaaccc gaccgaatca gtggccgttg atgtcgctta tgaaggctcc ggcagcggtg
     1201 actggcgcac tgacggtttc atcgtgggtg tcggttataa attttgatta gtaacacagc
     1261 gttatgacag cccgccggtt caggcgggct tttttgtgga gtgggtatgg cagcagtaca
     1321 aatatcaggc gtgctgaaag atggtgcggg aaaaccaata cagaactgca ccattcaact
     1381 gaaagccaga cgtaacagca ccacggtggt ggtgaacacg gtggcctctg aaaatccgga
     1441 tgaggcaggg cgttacagca tggacgtcga gtatggtcag tacagcgtca ctctgttggt
     1501 ggagggattc ccgccatcac atgccgggac catctccgtg tatgaagatt ctcaaccggg
     1561 tacgctgaat gattttctcg gtgccatgac ggaggatgat gcccgtccgg aggcactgcg
     1621 tcgttttgaa cagatggtgg aagaagcagc gcgtcacgca gaggaggcga agaagaatgc
     1681 cggagaagca gagacgtccg cgaggaatgc cggcatatca gccagtaagg cggaagcgag
     1741 cgccgcaaat gctgatactt cagcagagga tgcatcggag tcagcccggc aggcggcaga
     1801 aagtgcagcc tctgcaaaga agtcagagga agcgtcctcg tcctcagcct ctgaggccgc
     1861 tcaaaaagcc agtgagtcat tacaaagtgc aacagatgcc gagttgtcaa aaaagacggc
     1921 agaaagtgca gccggtaatg cagccaggga tgcaacgacc gcaacagaaa aagcccggga
     1981 atcagcagaa agcgcacagt cagcggaaca aagcagaata gcggcggaag acgccgtaaa
     2041 cagaattccc accgtggtgg ggcctcccgg accaaagggg gaaccgggtc ccgcgggtcc
     2101 tcaggggccg aagggagata aaggagagcg tggagacacc ggtccggcag gggcaaccgg
     2161 tgaacgggga ccgggaggag atacaggtcc ggcaggtccg caggggccga aaggcgacag
     2221 gggagagcgg ggagagaccg gtctgacagg aagtacaggt ccacaggggc caaagggaga
     2281 taccggggca acaggtccgg caggaccgca gggaccgaaa ggggaaacag gtgcggctgg
     2341 cccggtgggg gctaccggac ctcagggggc gaagggcgac ccgggggaga cacaaatacg
     2401 gttccgtctg gggccgatga gaattattga gacaaacagc tatggctggt tcccgggtac
     2461 agatggtgcg ctcatcaccg gactgacctt tcttgacccc aaagatgcca cacaggttca
     2521 ggggatgttt cagcatttgc aggtcagatt tggtgacggg ccatggcagg atgttaaggg
     2581 actggatgaa gtgggcagtg atacaggcag aactggagaa tgacatgaat attttgagaa
     2641 agcttatgca gagtctgtgc ggttgcggaa agcatgatga ctgtgaaaac ggacggtcac
     2701 ttacagcaca attgcgactg ggaccggcag acattctgga gtcagatgag aatggcatta
     2761 ttccggagca ggacagggta atcacacagg tggtgatact ggatgc
//
LOCUS       DAZKFZ010000344         2801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000344 DAZKFZ010000000
VERSION     DAZKFZ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..570
                     /locus_tag="ACHOQW_004776"
     CDS             <1..570
                     /locus_tag="ACHOQW_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9575677.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..>2801
                     /locus_tag="ACHOQW_004777"
     CDS             631..>2801
                     /locus_tag="ACHOQW_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9575678.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTP"
BASE COUNT          604 a          738 c          921 g          538 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaattatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac c
//
LOCUS       DAZKFZ010000345         2788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000345 DAZKFZ010000000
VERSION     DAZKFZ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            205..1371
                     /gene="nhaA"
                     /locus_tag="ACHOQW_004778"
     CDS             205..1371
                     /gene="nhaA"
                     /locus_tag="ACHOQW_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP9575679.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1437..2336
                     /gene="nhaR"
                     /locus_tag="ACHOQW_004779"
     CDS             1437..2336
                     /gene="nhaR"
                     /locus_tag="ACHOQW_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP9575680.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2374..>2788)
                     /locus_tag="ACHOQW_004780"
     CDS             complement(2374..>2788)
                     /locus_tag="ACHOQW_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP9575681.1"
                     /translation="FEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSD
                     VEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPH
                     EGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          651 a          613 c          712 g          812 t
ORIGIN      
        1 cctcacctta agctatgatt atcagggctt agggtcaccc gtcagcgctt acagccgtca
       61 aaaacgcatc tcaccgctga tggcgcaaat tcttctatag atcgtaaaaa acgaattatt
      121 cctacactat aatctgattt taacgatgat tcgtgcgggg taaaatagta aaaacgatct
      181 attcacctga aagagaaata aaaagtgaaa catctgcatc gattctttag cagtgatgcc
      241 tcgggaggca ttattcttat cattgccgct atcctggcga tgatgatggc caacagcggc
      301 gcaaccagtg gatggtatca cgactttctg gagacgccgg ttcagctccg ggttggttca
      361 ctcgaaatca acaaaaacat gctgttatgg ataaatgacg cgctgatggc ggtatttttc
      421 ctgttagtcg gtctggaagt taaacgtgaa ctgatgcaag gatcgctagc cagcttacgc
      481 caggccgcat ttccagttat cgctgctatt ggtgggatga ttgtgccggc attactctat
      541 ctggccttta actatgccga tccgattacc cgcgaagggt gggcgatccc ggcggctact
      601 gacattgcct ttgcacttgg tgtgctggcg ctattgggaa gtcgtgttcc gttagcgctg
      661 aagatctttt tgatggctct ggctattatc gacgatcttg gggccatcat tatcatcgca
      721 ttgttctaca ctaatgactt atcgatggcc tctcttggcg tcgcggctgt agcaattgct
      781 gtactcgcgg tattgaatct gtgtggtgta cgccgcacgg gcgtctatat tctggttggc
      841 gtagtgttgt ggactgcagt gttgaaatcg ggggttcacg caaccctggc gggggtaatt
      901 gtcggcttct ttattccttt gaaagagaag catgggcgtt ctccagcgaa gcgactggag
      961 catgtgttgc acccgtgggt ggcgtatctg attttaccac tgtttgcatt tgctaatgct
     1021 ggcgtttcac tgcaaggcgt cacgctggat ggcttgacct ccattctgcc attggggatc
     1081 atcgctggct tgctgattgg caaaccgctg gggattagtc tgttctgctg gttggcgctg
     1141 cgtttgaaac tggcgcatct gcctgaggga acgacttatc agcaaattat ggcggtgggg
     1201 atcctgtgcg gtatcggttt tactatgtct atctttattg ccagcctggc ctttggtagc
     1261 gtagatccag aactgattaa ctgggcgaaa ctcggtatcc tggtcggttc tatctcttcg
     1321 gcggtaattg gatacagctg gttacgcgtt cgtttgcgtc catcagtttg acaggacggt
     1381 ttaccgggga gccataaacg gctccctttt cattgttatc agggagagaa atgagcatgt
     1441 ctcatatcaa ttacaaccac ttgtattact tctggcatgt ttataaagaa ggttctgtgg
     1501 ttggcgcagc ggaggcgctt tatttaacac cacaaaccat taccggacag attcgggcgc
     1561 tggaagagcg cctgcaaggc aaattattta agcgcaaggg acgtggtctc gagcccagcg
     1621 agctggggga gctggtctat cgctatgccg ataaaatgtt taccttgagc caggaaatgc
     1681 tggatatcgt gaactatcgc aaagaatcca atttattgtt tgacgttggc gtggctgatg
     1741 tactttccaa acgtctggtc agtagcgtgc tgaatgctgc agtagtagaa ggcgagccga
     1801 ttcatcttcg ctgcttcgaa tccacccacg aaatgctgct ggagcaatta agccagcata
     1861 aactggatat gatcatttcc gactgtccga tagattctac gcagcaggaa ggcctgttct
     1921 ccgtgagaat tggcgaatgt ggcgtgagtt tctggtgtac aaatccaccg ccggaaaaac
     1981 cgttcccagc ttgtcttgaa gaacggcggc ttttgattcc tgggcgacgt tcaatgttag
     2041 ggcgcaaatt acttaactgg tttaactccc agggattaaa cgtagaaatc cttggcgagt
     2101 ttgatgatgc cgctttgatg aaagcttttg gtgcgatgca caatgcaatc ttcgttgccc
     2161 caacgcttta tgcatatgac ttttatgccg ataaaactgt cgtagaaatt ggtcgcgtcg
     2221 agaatgtgat ggaagagtac catgctattt ttgctgagcg gatgattcag cacccggcgg
     2281 tacagcgaat ctgcaatacg gattattctg cgctttttag tccagcggtg cgttaatcgg
     2341 cagctccccc aaagttaagg tgggggagat agattagttg tacattacca cgattttgac
     2401 tcggctcatt atttgcccgc ttgagacatt gtttccatat gtacgcgggc gaataaatag
     2461 aggaatctga ttacttcctt catggggatg ctgaaaagag tagtaattgc ttttaccata
     2521 ctggatttca ttcgtaccat cattggagag ttgaaagcct atttgacccg caccgtcctc
     2581 actgttttct aactcatttt ttaatatgct ggttcctgac tcctgtatcg tcactggttc
     2641 aaagtaatat tcaacgtcac tgggttcaaa aactaattca ccttgctcgc cctggatgca
     2701 gtcaaatctt atatgatgaa actgataatt ttcattattg atacgagaca gtacaaatcg
     2761 tccagtgtcg atggattgat cttcaaaa
//
LOCUS       DAZKFZ010000346         2777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000346 DAZKFZ010000000
VERSION     DAZKFZ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..546
                     /locus_tag="ACHOQW_004781"
     CDS             <1..546
                     /locus_tag="ACHOQW_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9575682.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..>2777
                     /locus_tag="ACHOQW_004782"
     CDS             607..>2777
                     /locus_tag="ACHOQW_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9575683.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTP"
BASE COUNT          603 a          714 c          933 g          527 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacacc
//
LOCUS       DAZKFZ010000347         2774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000347 DAZKFZ010000000
VERSION     DAZKFZ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..200)
                     /locus_tag="ACHOQW_004783"
     CDS             complement(<1..200)
                     /locus_tag="ACHOQW_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575684.1"
                     /translation="MSQPLSEILTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDK
                     GRDVVGYKSDPNVEGYSWDNYQ"
     gene            complement(204..1385)
                     /locus_tag="ACHOQW_004784"
     CDS             complement(204..1385)
                     /locus_tag="ACHOQW_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9575685.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            1872..2306
                     /locus_tag="ACHOQW_004785"
                     /pseudo
     CDS             1872..2306
                     /locus_tag="ACHOQW_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2348..>2774)
                     /locus_tag="ACHOQW_004786"
     CDS             complement(2348..>2774)
                     /locus_tag="ACHOQW_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9575686.1"
                     /translation="FDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSA
                     PWRLKDISSRLYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKV
                     CGYASVSYFTYVFRRYFGVSPSQYSQRSSESKILTHQGI"
BASE COUNT          808 a          659 c          554 g          753 t
ORIGIN      
        1 cattgataat tatcccaaga ataaccttct acgttaggat ccgatttata tccaacaacg
       61 tctctacctt tatctccagc tcctcccaaa cgttcgctcc acgggtaatc atctgattta
      121 cagacaataa gccaatcatg gacaaatacc tcccattgtt catcatccca tgtcaatatt
      181 tccgaaagtg gttgagacac aaattaactc ctttttagtc tttcaaatat caaattactg
      241 taccagtcca tcatagactt ccttccacta aaatattgag catggttata agtcccccta
      301 atagaatttt tatctacatg agccaactgg atttcaatcc aagcactctc aaaaccttgc
      361 tcatgcagga ttgtagaaag ggtatgacga aaaccatgtc cagtgacttt tcctccatac
      421 ccaatacgct taatggcttg atttatactc gcttcgctca ttggcctatt cggatcgttc
      481 cgccctggaa aaacataacg atagttccct gtcatgatct tgagttcatt gagtaaatct
      541 accgcttgag atgataaggg cacaagatgt ggcctacgca ttttcatcct ttcagcagga
      601 atttcccaaa tagcgttatc cagatcaaat tcttgccata atgccgcgcg taattcgatg
      661 gttctcacac ccgtaatcat cagtaatttc gtggctatct ggacaagctt actcccggag
      721 taaccctcta aggcacgtag aaaatcaggt atctcatcag cttttaggaa tgggaaatga
      781 ttggattggt gtacttcgag agcgctggag agatcagccg caggattgta ctccgcccta
      841 cccgttgcaa ttgcgtagcg aaacacttcg gagcaacgct gccgcacttt gcgcattttc
      901 tccaacgcac cacgtttctc aattttacgc agaacgttca gcagctctag cggtttaatc
      961 tctcccacag gccttgttcc gacataagga aaaatgtcgt tcttaaacgc ttccatgatg
     1021 tctgatgcat atcccgccga ccatttagca gatttaagtt gatgccactc tctggctatc
     1081 ttttcgaagg cgttctctga ctctgtttgc atagctagct tttgctcttt tcgaacctca
     1141 ctagggttct ttccttctgc cacaagtttt cgagcttcat cacgacggga acgagcatcg
     1201 gcaagggtga tcgttgggta aacaccaagc gagatcattt tgggtttgcc ggcatagcga
     1261 tagcggaacc gccaactctt gcttccatta ggttctataa gtaatgacaa ccctagccca
     1321 tctccaaatg tataggcttt agcttcaggc ttagcgcggc gaatctgcat atcgtttaaa
     1381 ggcatgtgta taggaatcca gaccgaacag gaacatatac acaatcctat acacattcag
     1441 taccggattc tactggatgg ttacggacta ctatggactt aaaagaataa aaaatgttat
     1501 aaatcagaga ttttatggac taatacggac gtttgaggaa gttgagatgg tgccgataat
     1561 aggagtcgaa cctacgacct tcgcattacg aattataaga atccgcttct aattcaaagc
     1621 attaccccat caacactgcg ctcacacgtc ccaccacatc aaaacatgta aagccttgca
     1681 agccattgcg aggccttatg tgtctcagtt ttgtcccacc ttgtattacg acttgcatag
     1741 ccaatgaaga taaacgtgac gacaaacggc gcagcagtct tcttttcttt cacgacttct
     1801 ccagcatgca tacctttctg ccatgactga agtgaatgcc tgttatgagc aaggattggc
     1861 ccagatgaat cgccctggac tgttgcgatc gtgaagccat agactgggcc gcaagcacgg
     1921 gaggttacga taaagcgaca gtgcaggatg tcatgttagg agcgatagaa aaacgctttg
     1981 gcgataaagt accggaacag tccatccagt ggctgacaga taacggttca gcgtatagag
     2041 cgcatgaaac acggcagttc gccagagagt tgaatctgga gccctgcaca acggcaatca
     2101 gcagtcctca gagtaatgga attgcagaaa ggttcgtgaa aacgatgaag gaagactaca
     2161 tcgcgttcat gccgaaaccg aatgtaagaa cggcattgca taatcttgca gtggcgatcg
     2221 aacattacaa tgaaaaccat ccgcacagtg cgttgggtta tcgctctccg cgagaatatc
     2281 gacgtcagcg ggtaacgtta acttaagata ccccccctgt ctggaaataa gggggcaaga
     2341 acaatgatca gattccctgg tgagtaagaa ttttactttc tgaactcctc tgagagtatt
     2401 gacttggaga aacaccaaaa taacgtctaa atacataagt aaagtatgag acactggcat
     2461 aaccacagac cttagctact ttactaatag gataacaacg agtgctgagt aaatattcag
     2521 ccatttgcat cctctcatca agtatgatct tactgaatga taccccctct tctttcaatt
     2581 tcctctttag taaactttcg ctgagatata atcttgatga tatatcctta agacgccatg
     2641 gtgcagataa atccgtatga ataatcgttt taactttagc ccctgtgctt ttcaaacatc
     2701 caaacagaaa tactccaaat tgtttttcag aagaaaatgc agagagacat gtaaaagcaa
     2761 tagactcatc aaaa
//
LOCUS       DAZKFZ010000348         2735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000348 DAZKFZ010000000
VERSION     DAZKFZ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..2428)
                     /gene="yeeJ"
                     /locus_tag="ACHOQW_004787"
     CDS             complement(<1..2428)
                     /gene="yeeJ"
                     /locus_tag="ACHOQW_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HFP9575687.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKS"
BASE COUNT          645 a          669 c          682 g          739 t
ORIGIN      
        1 tacttttcag atcaaaagtc gccagaccat taacatccgt ttttgcggtg ttttgattgt
       61 taaaggaagt tgccgatccg cttaataccg caaacgtgac ggtatgatca ttgattgggt
      121 tgcttccttc atcagcgaca ttgaccgaga cggtgtttgc tgcattctca ttggcgagca
      181 caccattatt gctggcagat aatgtcgcaa ttttcgctga ctgcgggttg gcgtcgatga
      241 taaatccagc ggtatgcaaa tcttcattcc agttttgcat taacagcttc gcagtaagcc
      301 cactgccttt ggtataggcg gtataggttg ccttatagac gccatctgcg gtttctttcc
      361 agtctgtagt gacaccaggt ttcacgttgt cgatgctgac tgcggtattc agttgctgtt
      421 tttgctcctt aacaggtttg tcattttcat ctctcagttc taccgtcacc tcgataggat
      481 tcccggagag ataacggtcc ttatcaatct taattgacga gtgagtccgg gatgacgaaa
      541 cagaaatgat attcaccacg gcgggggctt tagccgcatc cacaccgttc agctgtggca
      601 tcagcgtcag cgtgccagac atcgctcctg tggtcaggat ctgggtatag cttccgtcac
      661 cattatcttt ccagtcagaa agggtgatgt cctgaacacc ttcgtgacgc gtcgagagca
      721 ccagcccgat aacaggatta cctgctgcat catgcgcaat aaaagtcagt gtggcggttg
      781 aatgggaatc cgcgctcaac gtctggctac ttaacgatac cgaggaatct ttctggctta
      841 gcgtaggagc ctgaacgact accatgctct gttcacgatt cgaaaaattg cctttgacat
      901 cttcagcggt gacttcaatc ggccaggtgt tatcggtttc tggcgtactg gtgaaccggt
      961 acgccggcag ggtaaccaga atatctttac ccgttgtaac cactttacca ccggcagctt
     1021 ccagagcggt ggcttcgacg ttatagcctt tcagggcata tttggtttgt agcgacgaaa
     1081 ccagtgattt cacttctcct gacttccctg tcacggggtc tgtcagggtc aggcgaacca
     1141 gttctttttt gcgatattcc agaacgatgt tgttgttgcg atccaccaga tcaaaacggc
     1201 tgcctgcaag gctacgccgt gcatcgactt cattcgggtc aagctgtttc tgcatcgcgc
     1261 ttccaggttg ccaggtaaaa tcgacggcaa aacgggtgtc attttcgccc tgtttaccct
     1321 ggcgttgctc cgcgctgaag gtcatcagcg ggaagggggt atagttaagt ccagcggtta
     1381 tggcatgagg attactttgc cgatcatctt tatcgaacag ggccacttca tcgccataat
     1441 actgttcata gaccagttta ccgccaaggt gcggccaggc gggtagccag ccttctgcgc
     1501 gtacatccca gccattggcc gggcgtgctt cataatcgtt gtccagttca ggtgcgctgc
     1561 gccagttggt cagtcgcaaa tagccgttac tgcttaattt tagatagtcg cgccagtact
     1621 ccgcgccaat gccggcgcgg gagtggtaac ggctaagatc gtggtcgaaa aagaagttga
     1681 tgcccgacat ccatgtggga gtgaaatgac gccaacccaa gccgttgtta atctgcgtac
     1741 gctcgtcagt acgatggaga gtatgctgac tgaaaaagag attatcaggc gtttcatacc
     1801 acggatggag aaaatcgaac tgggagttct tcaggctaaa atcttcatcc acgcccagcg
     1861 tgattcttgc ggtaccgaag cggcttaacc agtctgtcat tgcgcctgaa gcctgagaag
     1921 aggcccatcc acgcgccata tttgccgctt gctcgctgtt catatcctcg gcgagcagag
     1981 acccgatttg ctgtgaagta ctggctatct gttgctcaag gttgccactg ctattacccg
     2041 gtggcggggt taaattattt tcactaactt gtgccgggac atccagttca tcaccctggc
     2101 ggacattatc aaaacctcga gcaaacgtac gaaactggtt gagtttgcgt aactcagcca
     2161 ccgaaatacc gaaacgttcg gcaacgcttt gggccgattc cagcgctcca agggtgtagg
     2221 gcaccgtatt ggcatttgca atggcaattt gtgcaggaac tggttgttgg gttgcggcgt
     2281 ttaccacacc ttgtgctgcc gcagccatag ggaacacaag ttgagttatc aggcagatac
     2341 ccgcagttaa gcggcgtagt ttaattccca tcccgcataa gatttgtcgg tcatttattt
     2401 cttctccact tctcttcttc gtagccatgc ttaccttccc tgaacgagct aacataaatg
     2461 cgccctttct agcacagcga aaaaatgtaa atcctgatag caatatgatt gaaaattaat
     2521 aacttttcgt taggcagttt tggatgtgag ttgcaagagg ggagactact gaataactca
     2581 agttttataa tcgaggggaa aatggtgatg gcgttcatag caaaacgccc tcaaccataa
     2641 aggtcgaggg cgcttaagat gttaaaaacc cgctatccgt taaaaaacaa tgttcaacta
     2701 aggtcagtga cattgcgcta aaaaagcgaa ttttt
//
LOCUS       DAZKFZ010000349         2730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000349 DAZKFZ010000000
VERSION     DAZKFZ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(438..614)
                     /locus_tag="ACHOQW_004788"
     CDS             complement(438..614)
                     /locus_tag="ACHOQW_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575688.1"
                     /translation="MVNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIAT
                     EMCGISPGTVTCKR"
     gene            789..992
                     /locus_tag="ACHOQW_004789"
     CDS             789..992
                     /locus_tag="ACHOQW_004789"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFP9575689.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(924..1397)
                     /locus_tag="ACHOQW_004790"
     CDS             complement(924..1397)
                     /locus_tag="ACHOQW_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575690.1"
                     /translation="MQVMPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGT
                     FFSLVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRT
                     VTEVNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1317..2057
                     /locus_tag="ACHOQW_004791"
     CDS             1317..2057
                     /locus_tag="ACHOQW_004791"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9575691.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            2291..2698
                     /locus_tag="ACHOQW_004792"
                     /pseudo
     CDS             2291..2698
                     /locus_tag="ACHOQW_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          721 a          680 c          646 g          683 t
ORIGIN      
        1 gatttgaaca cactgacaaa aaaccaagca tggtaataaa ccgaactccc cacaaacttc
       61 tgttgcctca gatactcccc cccctttcct caggcacaaa tgttgttcca taacgaacca
      121 gccctgttcc tttaaactct gcatcaatgg cataatgtat actcaccaga ctgagtttgc
      181 tcctgcatca ttatttcgat acgttaatgc attgttcatt gcatccggca ttccccatcg
      241 aatatttact ttgttatatc agtcagccca taaccaggta aatttttaag gcttactgac
      301 tgaatattca aaatgtgtta catgttatca aacctttaaa gaataacctg ctcttcttca
      361 ttggccggat ttagctcaat cttgtcagca aaactaatat aaagccgttc ataaagagaa
      421 ggatgtagct tcgttattta tcttttacat gtaacagtgc caggggatat tccgcacatc
      481 tctgtggcta tcatccccac cacgtctcca gtggcagcac ctgcggcact ggcacgggcg
      541 ttatttccct gtaccaccgc cagtgcagca ttcaccacgg catgagctgc cacatttgca
      601 gtcttattca ccacacctgt aacctgtctc ttatacacaa atccccaccc ggggatttgt
      661 gatgtctgtc agtctgcttt agggggactc tttccatccg gtagtttcct tcctccccgg
      721 cagcgatttt ttgaactttt tcttcacccg ttgatcttct cttatatttc attttcttcg
      781 ggtctgttat ggtactttct gattgttatt cactggctaa cgagcagtct ggacatgccc
      841 gtctcggtga tccgcgtcgt acacgacgcc ttgtgtcact gacctcttct ctcgctcagc
      901 atgcaggtct ttctattgtg aaatcatccc acttcactgc tcaggttgaa ggtgcgtacc
      961 gcctgatacg taacccgtcg gtgtccccgt aagccattgc cgaagccgga tttactgcca
     1021 ctgcaagggc atgtgaggca catccgctgc tcctcgcgct ggaagatacc accaccatta
     1081 acttcggtca cagtacggca tttgatgacc tggggaatac caccactaac ccgaagacgc
     1141 acgggctgct ggctcactct gtcctgatgt acgcccccga cagcgccctg ccagtcgatc
     1201 ttatcgaaca gcagtgctgg agtcgtgcca cggacaccta cggtgttaag caccagcgaa
     1261 aagaacgtcc ctgtgaagaa aaagaaagtt accgctggca gcaggcttcg gagcgcatgg
     1321 ccgaacggct gagggagata cagaaacagg taatcactgt ctgtgaccgg gaagcggata
     1381 tctggcatta cctgcattat aaagtcagcc gcgggaagcc acacgctgaa tgtgatgcaa
     1441 aaaggcgggc gggcagcccg ccaggcccgg atgctcatca gttacagcga agtcagcata
     1501 aaaaatcccg acaacagcgg ccaggcgctc tcgctcacgt atgtctgttg cggggagcag
     1561 gagaggacgg agcctgctgg tatccgctga catcagaaaa agtggcgagt gccgccgatg
     1621 cgcgacgtat cgtcagccat tacgagcaac gctggctgat agaggaatac cacaaagcgt
     1681 ggaaaagtgg tggtacatgt gtggaatcgc tgcgaatgca gacccgggat aaccttgagc
     1741 gcatggtggt tatcgcggtg cgggtgctgg gcctgcggca gggaggtatc agtgaagaaa
     1801 cacaaaatga cagctgtgag aaaatactga cgccgacaga gtggaaacta ctgtgggtta
     1861 agctggaagg aaagcagctt ccggcgcaaa caccgaccct gaaatgggcc tgtttgaaac
     1921 tggctaaact ggggcgatgg catgacagca aacgcacaag cagccccggt tgggtagtta
     1981 tgtgggatgg ctggttcaga catcaggata tggctgaggg ttacctggtg atgaagtctc
     2041 ttgatcagga gatctgatca agagacaggt caaagatggg ggacaacgtc tggtgctgta
     2101 taacagcaca ggcctcacac tcttgacgcc cgtggaatat attagagaac aaaaatttca
     2161 atatgtggtg gacgcataca gaggattgac agcattccca tactaattgt taagattcaa
     2221 attgaagtat atctatctgt cattttgcaa atgatatgcg gtagctttac ctaactatca
     2281 tgaacatttc gaaaaatcat ccacaaagcc ccgaagaata aagcactatc gcccgataaa
     2341 gacctgtaag ctcagtggca tggatactat tgagctcaga atggcgagct gtaccgttat
     2401 ttcattacca tgctcgacga acacagcaac tactctctgg ccgtactatc gcttaaaagt
     2461 gatatcatca ggcatttctt ctgctgtgta gcccagttgt tccttgttgg tatcaaccag
     2521 atcatcactg atgatgggca aagagttctc gggaaaattc gacaaaacgc tacaggaagc
     2581 caccgtcggg ttcgtatgga cttatctcta tacgccgaaa ataaatgcca taagtgagca
     2641 ttttaaccga acactgaggg aacaatttat tgagtttaat gaagaattgc tctttgaata
     2701 gctgaatatg ttcaatctga aactggctga
//
LOCUS       DAZKFZ010000350         2728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000350 DAZKFZ010000000
VERSION     DAZKFZ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            133..>2728
                     /locus_tag="ACHOQW_004793"
     CDS             133..>2728
                     /locus_tag="ACHOQW_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFP9575692.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGII
                     MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKL
                     LLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVA
                     EKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKD
                     KATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKD
                     EFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNALCEADPEQRK
                     KNFNELIFE"
BASE COUNT          797 a          497 c          599 g          835 t
ORIGIN      
        1 cggtgtagcg ctcatgattg taacaagttt tttcgagata taaatgattt gtctcatttt
       61 tagcgagttc atcgggttga gcttttttat gatgatagta acctgttacg aattgtatca
      121 aaggaaggca ggttgcttgt gaaaactcca gttaacccac ttttgcaatg gcttaacatg
      181 ttttttagtc gccgttcatt atccggagct gatggacggg cgttatatgc ttaccgttgt
      241 actgatacag agtacgaaag tttggcagaa ctactgcgta catatgcccc ccgtagttat
      301 ccaagaacga tattcatttc ctatagcgat gttctattta gcatatatgc cgcagagttt
      361 atccgccgga ctcatacggt cggacaccct aaatgggaca caattttaga ttctattaac
      421 tggaaagttc cgtatgttca tcgacagaag ctggttaatg atggcattcg ctactggaaa
      481 agaaagataa gaaacctggg acaagcttcg ggttatctgc atacactggc ttgtgaaggt
      541 ggtttgccta tccgcatgat tgaaaatgag agcggttatt tgattaccta tttcagaagg
      601 atatatcagg cgcttcgtgg gcaatcatct caatatcctg ccgctaaaat tgcacaagaa
      661 ttaggcgata cgattcctgt cacaatgcaa aacgaactgg tctatgaaat agccggtgaa
      721 ttctgcgaga ctctctgccg attacttagt gagcatcctc ctcatagcag tgatcccgtt
      781 tctgcattac gtaagctctc tccagactgg catcttcaac ttccactcgt tctccccgaa
      841 gcgaatgctg cagagattgt aaggcgatta ctttctcaat cttctgaaat acgcagtgca
      901 agtagtcttc aggttgaaag aatctgggtc gatgttgatg acagttggta ttgcgatgcc
      961 cggttccgat tcccggccac aatgcgtaca gaacagttaa cctctttgtt tgaatgccat
     1021 attcagccgg agcagacccg actcatcatt tcaggaaaat ggaaaaatgg cggggcaagg
     1081 ttggcaatgc taagccgtta tgagcagcaa gattggcggg tcgagttatt acctattgcg
     1141 atgcaaaaac tctctggtgc agatgcaatg gccgaaatct cattgtcgct tcatgaaggt
     1201 ccaattctgt taggtcacac aattccaaaa ggcggttatg aacttactga agaacttccc
     1261 tgggtttttg aagcgatgaa tgagagtgaa tcgcagctaa aacttgttgg tatggggtct
     1321 gtgagttcca ggctaaatgc tctgtttatt tcgctaccga aaaatagcca tttagatatt
     1381 agtggagaag gtgagtttga tatcccaagg ttgctgaaaa acagtgaacg cagcttaact
     1441 aaaataagtg gagtatttag cgttgtttta catgatggtg ctgtttgtac aattcgcacc
     1501 cagcaacttt atgattctgc gattgaatat tatattaaat cgacagaagt tgaactggtt
     1561 aaatcggatt atcctgtcca tcgagcctgg ccgaagatcg gttggaaaaa ggatctgcaa
     1621 tatggcattg taccggagaa agaacttttt tggcgttcca ttcgttcagg taataatgcc
     1681 tggtattctg tcgcatcaga aatgccaaaa ggacagatag aagtccggcg aatagttaat
     1741 gatgaggttt tatttagcgg taaagttgta gttttaccag cagacttcga tattaatatt
     1801 atacctgaga gtgctcagca aggcattatt atgctttctg gtataacgga tactagaatt
     1861 gataaatatt caaacaatga aaaagtaaca cttaagtccg attattcaca aaacgagtgt
     1921 gctatttatt ataattcatc gctgatgctg gaaaataccg ttgatttacg ggtctcctgg
     1981 aaagatggtt ctaatcttaa actattatta cctaagccgg ttagcggtgg ccgatttgta
     2041 actaatgatg gttctgttca ttttgatggt gtggcatcta tagcacattt gcatggaata
     2101 gatgctgagt tattaaccat atcatgtgct ggaagaggat atcttaatat cgagttattg
     2161 gatgaaaatc cagtagctga aaaattccgc tatttacatg ccgaccttcc tcttttatca
     2221 ggacgcaatg acaaattaca acaaatttca ctttatgaga actataattt gctaaatgct
     2281 atgctggcat gtgcatggaa cagcaatagt acattatgtg ttgattttta ctctgacaga
     2341 tttggaaagg ataaagcaac actcaatatt aaacgctacg atggtagttt tattgaacac
     2401 gatcaagggt tactggttga tataaaaaat tctgttgttt ttcctgcaaa tagaatagac
     2461 gaactggttg ttgatgctat ttctcttaag aatcctggct tacacatatc attgttaaaa
     2521 aaagatgagt ttgcttatga tctttcagct ctgaatgttc aggatagtcc atggttaatt
     2581 gtgggaaaac ttgatggtac agctcgcatt gcaccagtaa ttaaatggat gctacctgta
     2641 ttgcagacaa atgatttatt actaaatgct ctatgcgaag cagacccaga acagcgcaaa
     2701 aaaaatttta atgagctaat ttttgaaa
//
LOCUS       DAZKFZ010000351         2660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000351 DAZKFZ010000000
VERSION     DAZKFZ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..170)
                     /locus_tag="ACHOQW_004794"
     CDS             complement(<1..170)
                     /locus_tag="ACHOQW_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFP9575693.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAV"
     gene            414..671
                     /gene="ynaJ"
                     /locus_tag="ACHOQW_004795"
     CDS             414..671
                     /gene="ynaJ"
                     /locus_tag="ACHOQW_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP9575694.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(721..1671)
                     /gene="uspE"
                     /locus_tag="ACHOQW_004796"
     CDS             complement(721..1671)
                     /gene="uspE"
                     /locus_tag="ACHOQW_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP9575695.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1823..2575)
                     /gene="fnr"
                     /locus_tag="ACHOQW_004797"
     CDS             complement(1823..2575)
                     /gene="fnr"
                     /locus_tag="ACHOQW_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP9575696.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT          683 a          619 c          638 g          720 t
ORIGIN      
        1 acagcactga taattatggt gtagattaaa aattgcaccg catggaataa aaagcctttt
       61 cttttacgat ttccgcggcg aaaccaaaag ctcatcagaa tcaatgctgc gcagctaccg
      121 aaaataatga ccagattaag cgcattattt gtaaacagtt cagcgatcat tgttttatca
      181 ggctcctcca gataattgtc gtcatgccgg aaacccctgg cggggctatt ttaccgcgac
      241 aattcattca gatcatcaat agtcagggaa ggaagtagca acattagcta actaatcaat
      301 gcgttgattg taaatccagc taagaggtga ggttttcaga gcagacaatg gtgaaatgtc
      361 atggtattgt tacgtttagg taacaagaaa tttgtctgca caaggattac atcatgatta
      421 tggcgaaact gaagtcagcg aaagggaaga aatttctctt tggtttgttg gcggttttca
      481 ttattgcggc gtcggttgtg actcgcgcga ctatcggcgg cgttatagaa cagtacaata
      541 ttccgctgtc ggagtggacg acatcaatgt atgtgattca gtcatcgatg atttttgttt
      601 atagcctggt ctttactgtg ttgctggcaa tcccgttggg aatttatttc cttggcggcg
      661 aagagcagta agtaaaaaat aggcccgata actcgggcct tgtcagttat tgaggagtcg
      721 ttaatcgtct tcttcgtcat ccagttcaac gggtgtctga tactggtcag gtttaatgac
      781 caacagatcg cagcgaagat gatcaatcac ctgttccgcc gtgttgccga ggaatgctgc
      841 tgaaataccg gtgcgtccta ccgtgcccag aaccacaatc cccgcctgta agtgctccgc
      901 caaatcagga atcacctctt ctggcagacc tttttctacg tgcgtcatgt tttcattaat
      961 gccgaatttc tgccgcaggg ctttcattgc cagcaaatgt tgcccacgaa tggcatcgtt
     1021 ataaacgctc ggatcaaatt ccggcagttc aatcgcgata ttaattggcg ttaccggata
     1081 agcgccaacc agatgaactt cggtatggtt gacttgttct gccagttcga tcgtctcttt
     1141 gaccagtttt tcattgagcg cattatgata cggctcttca ctggcgagat tcaccgccac
     1201 cagcgcctta cctccttccg gccacggctg gtctttcacc atccacaccg ggcttgggca
     1261 tttgcgtaac aggtgccagt ccgttggcgt aaaaatcacc gcttccagac ggtcatgttg
     1321 gtgcgccatt tttagcacca aatcgtgccc gccgctgatc acttcctgaa tgatggcttc
     1381 gaaaggacgg ttatgccaga ccactttaat ttcaatggga acgccagcat tgagataata
     1441 ttttgcctgc tcgtggatcc aggctgtacg ctggctgatg acgccctgac gcatagcggt
     1501 acgttcgtcc ggggagagca gggtggtcat ttcgtatgag aagtcataga tcggcaaaaa
     1561 ggctttaatt ttgccaccaa tccgttgatg taaataaaca gctcgccgca atgctggttg
     1621 gtcgtcctgg ttaggatcga taacaacgag catgttctga tacatagcca tacagggtct
     1681 ccttacaaca actgtcaacg cagtttgtaa ttaaaagatt aacccatatc tggtgaatga
     1741 aacagtgatg aaccttctgc cagatcaata aatcagaaaa atttaatgat atgacagaag
     1801 gatagtgagt tatgcggaaa aatcaggcaa cgttacgcgt atgaccagca agctgggcca
     1861 gcgcatcgtt attttcgatg gtgatgtatt tacctttgac tgccagcata ccgcttttct
     1921 ggaagcgacc cagcagacgg ctgatggttt ctaccgtcag gcccagatag ttaccgatat
     1981 cgccacgagt catcgtcagg cggaattcac gaggggagaa gccgcgttgg gcaaaacgac
     2041 gggacaggtt gtagatgaat gcagccagac gttcctcggc atttttcttt gacaacagca
     2101 ggatcatgtc ctgatcgcct ttgatttcac cgctcatcag acgcatcatc tgctgacgca
     2161 gattcggcat tttaccggac aaatcgtcca gcgtttcgaa cgggatttca cataccatcg
     2221 aggtttccag cgcctgcgcg aagctcgggt gatggccgct gccgatggcg tcaaatccca
     2281 ccaggtcgcc tgctaaatgg aaaccagtga tttgctcgtc gccttgctca gtgatggtat
     2341 aacttttaat cgtaccggag cggatggcat aaagcgattt aagttcatca ccagccttaa
     2401 acagcgtctg gcctttctga ataggcttct tccgctcaat gatattatca agctgatcaa
     2461 gctcatgttc gttgagtgtg aacgggatgc aaagctggct gatgctgcaa tcctggcaat
     2521 ggatagcaca accgccagac tgaatgcgcc gtataattcg cttttccggg atcataggtc
     2581 tgctcaagcc gtaattgata tttgtcaatt ttaacatctt tttagggagc aagtaagtct
     2641 aagcaaacct taacagcaga
//
LOCUS       DAZKFZ010000352         2652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000352 DAZKFZ010000000
VERSION     DAZKFZ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            13..231
                     /locus_tag="ACHOQW_004798"
     CDS             13..231
                     /locus_tag="ACHOQW_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP9575697.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            233..790
                     /locus_tag="ACHOQW_004799"
     CDS             233..790
                     /locus_tag="ACHOQW_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9575698.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1081..1236
                     /locus_tag="ACHOQW_004800"
     CDS             1081..1236
                     /locus_tag="ACHOQW_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9575699.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1356..1700
                     /locus_tag="ACHOQW_004801"
     CDS             1356..1700
                     /locus_tag="ACHOQW_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFP9575700.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1822..2094
                     /locus_tag="ACHOQW_004802"
     CDS             1822..2094
                     /locus_tag="ACHOQW_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575701.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2096..>2652
                     /locus_tag="ACHOQW_004803"
     CDS             2096..>2652
                     /locus_tag="ACHOQW_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9575702.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          690 a          600 c          722 g          640 t
ORIGIN      
        1 gggcggtaag aaatgaaaca ttatttagaa aaaaattacc cacgaaagag cagaacaaca
       61 gagtttctgt ttttcattct gtttatagtg ttgatgatac cgatatcccc gctattactg
      121 gtctggataa ttggaaggac atttgaacca gttattgagc tatataccga tgtgacatgg
      181 gaatcattca gcgcactgca caataaaatt aatccgtata aggaaaactg atatgagcac
      241 tattaccaga gaacgcgcgg agattaaatc atacatcaca ggcttcctga gcgactcggc
      301 gcacgataac aagtcttcag acagcctgct ggctaatgtg tttcgtatcg cgctggcatc
      361 actggaagca gagccgatag caatggtagt gcctgatgaa atggatttgc ttacctgcca
      421 tctcgacggt gtaactaaaa catatgctga tggctggaac gcctgccgcg tcgccatgct
      481 tcaggccgga aactttcggg aaaataagaa ttcgtcaacc aacaattttc gggaaatctc
      541 ggaaacgtca accagatctc cgataactct ggatggctgg ataagctgta ctgagcgaat
      601 gcctgaaaag agccagaacg tgcttatttc gatgaatatc gatagcgagg ctgggccatt
      661 aatatattcc gcacgctatc tcggaggcac gttccggcgc ggaggtatag cagttagtcc
      721 gggtaatgat cttaggcaag caacccactg gatgtcgcta ccagaaccgc cgcaggaggt
      781 gaatcaatga cctggcctga agcattcaca acggtaggaa ttgcgatggc ggtggcgctg
      841 gtggtgtatt cgatttgccg ctggggataa aaacggtttg cgggaaaagg atagttaagt
      901 agaattgctg cgggtgcttg aggctatctg cctcgggcat gaacaccaac ggcagataga
      961 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa
     1021 tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
     1081 atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg
     1141 acggcactgg taacgaggaa agacctctgc gaggtgcgaa tccgaaccgg tcagacggag
     1201 gtcgctgtct tcgtagacta cgaatctgag aagtaagaga ccaggcgggg gagtaatctc
     1261 ccgccacctc tgatgtgtca ggcatcctca acgcacccgc gctttaccat actgaaaatg
     1321 ctgtttgaat gttcatctct gaaagaggac tatgaatgaa aaaggtattg attgcagcac
     1381 ttatttccgg tgtgtctttt ggcgcttttg cacagcaggg tggtttccag gggccagaag
     1441 cagagcgttc aacagtagcg caggcaaaag aactgaagga tgatgcatgg gttatccttg
     1501 aagggagcat cgttaaaaaa gtgggtgatg aacgttatga gtttcgtgac aatagcggga
     1561 caattgtcac ggatattgat gacagcgtat gggccgggca gaatgtttct ccgaaagaca
     1621 aagtaagaat tgagggtgaa attgataaag acctgagcag tgttgaagtt gatgtaaagg
     1681 cactgaaatt attaaagtaa ccgcccctgc ttgttaagcc cgtcttactg acgggttttc
     1741 tgtttgtaca ttccggcgta ttgccttaca attcgcgcag tcagcctgaa caactgacac
     1801 ctgctgtcac cggagaatcc gatgacacaa cacataaaat cccacaattc tgaagccgac
     1861 ccggaaatta agcaggggag gcgttttcgt gcgcctcagt atggctggtt tcactatctg
     1921 ttctgtacga tcgatgaggc agatatgctt caagaggcgt atctgcgtcg cggtgtccgt
     1981 gtggagcgga gtctgaacgc tgatcgtctg acctggaccg tttctgtata tcttcctgtt
     2041 cgtgcacatc tgccacggac acatgcctgc taccgtcagc gcgtctggag gtaatgtgcg
     2101 ggtattactt cgacctgttc tggttccgga actcgggctg gtggtcctta agcccggtcg
     2161 tgaatcattg ccagtttttc atcgcggcag ggtgctggtg gagccggaac cgaaaaacat
     2221 gcgggcgctg ccatctggag cggttcctgc tgttcgccag ccgctggcgg aagataaatc
     2281 actgctgcca tttttcagcg atgagcgggt gattcgtgca gctggcggcg ctggtgcact
     2341 gtctgactgg ttattacgtc acgtgaaatc ctgccagtgg ccacacggcg attatcatca
     2401 cagcgaaacc gttattcaca gttacggtgc tggcgcaatg gtgttgtgct ggcactgcga
     2461 caaccagctg cgcgaccaga cctccgaatc acttgagcaa cttactcaac aaaatctgac
     2521 agcctggatg attgacgtca tacgccatgt aatgaatggc acgcaggagc gggaattatc
     2581 gctggctgaa ttatcctggt gggcagtctg caatcaggtg gtggacgcat tacctgaggc
     2641 agtatcgcgt cg
//
LOCUS       DAZKFZ010000353         2614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000353 DAZKFZ010000000
VERSION     DAZKFZ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            418..561
                     /locus_tag="ACHOQW_004804"
     CDS             418..561
                     /locus_tag="ACHOQW_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP9575703.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            574..783
                     /gene="cspE"
                     /locus_tag="ACHOQW_004805"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             574..783
                     /gene="cspE"
                     /locus_tag="ACHOQW_004805"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9575704.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            949..1758
                     /gene="rlmA"
                     /locus_tag="ACHOQW_004806"
     CDS             949..1758
                     /gene="rlmA"
                     /locus_tag="ACHOQW_004806"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP9575705.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1755..2321)
                     /gene="mntP"
                     /locus_tag="ACHOQW_004807"
     CDS             complement(1755..2321)
                     /gene="mntP"
                     /locus_tag="ACHOQW_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP9575706.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2318..2440)
                     /locus_tag="ACHOQW_004808"
     CDS             complement(2318..2440)
                     /locus_tag="ACHOQW_004808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575707.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT          695 a          609 c          623 g          687 t
ORIGIN      
        1 tgcttattca tcaatttttc ttattttaaa tttccaattc ctttgggatt gacttctctt
       61 tggggtgatt gagagccgtt aactgcctgt tttatgagaa aaagtgatat aactttttat
      121 tcattgcata gcagaaaatg tgatattgca cgcactatgt aataacttct cccactgacc
      181 tggaacaact gaacttattg aactatgtta gaaactacgt cagtttaagt atctgcggaa
      241 ctgacaaggt taagcacaat gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa
      301 aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc
      361 ataattaacg ttgtatgtga taacagattt cgggttaaac gaggtacagt tctgtttatg
      421 tgtggcattt tcagtaaaga agtcctgagt aaacacgttg acgttgaata ccgcttctct
      481 gccgagcctt atattggtgc ctcatgcagt aatgtgtcag ttttatctat gttatgcctg
      541 cgggcgaaga aaacaatcta aggaattttt caaatggcaa agattaaagg tcaggttaag
      601 tggttcaacg agtctaaagg ttttggcttc attactcctg ctgatggcag caaagacgtg
      661 ttcgtacact tctccgctat ccagggtaat ggcttcaaaa ctctggctga aggtcagaac
      721 gttgagttcg aaattcagga cggccagaaa ggtccggcag ctgttaacgt aacagctatc
      781 tgatcgaatc cactgatctg aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg
      841 aatgcgaggc tttttactat gctttatctt cgctcctggc gttcggatat ttgcccgccg
      901 cgtgattcgc gttacacttg cggcctttag tatcctgccg gagttgtcat gtcgttttcc
      961 tgtccacttt gccatcagcc tcttacgcgt gaaaaaaaca gctatatctg tccccaacga
     1021 catcagtttg atatggcgaa agaagggtat gtcaatctgc tgcccgttca gcataaacgg
     1081 tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc
     1141 ggacattatc agccgctgcg tgatgcaatt gtcgcccaac tgcgggaacg gcttaatgaa
     1201 aaggccacgg cggtgctgga tattggctgt ggtgaagggt attacacaca cgcatttgcc
     1261 gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt cgaaggtagc gataaaagcg
     1321 gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt ccagccaccg tttgccgttt
     1381 tccgatacca gtatggacgc cataatacgt atttacgcgc cgtgtaaagc agaagaatta
     1441 gcacgagtag tgaagcccgg cggctgggtc attactgcca cgccgggacc gcgacatttg
     1501 atggagctga aggggctgat ttacaatgaa gtacatcttc atgcacctca tgcagaacaa
     1561 ctggaaggtt ttacattaca gaagagtgat gagttgtgtt atccgatgcg tcttcgcggt
     1621 gatgaagccg tcgcattatt gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc
     1681 tggcaaatac tggcagcaaa agaagtgttc gactgccaga cggactttaa tattcacctc
     1741 tggcagcgtt cttattaacc gtggaagtgc gtccagagga tctggacgcc gatgccgatc
     1801 agcaccagcc cgccgagaat ttccgctttt ttcccaataa ttgagccgat aaagcgacca
     1861 accatcatcc ctaatgtcga cataatcaag gttgcacaac caatggccaa tgcggtcgcg
     1921 ataatgttga cctgaaggaa agcaagacca acacccacag ccatggcatc caggctggtg
     1981 gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca
     2041 tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc cgaggaatat cagcagcaca
     2101 aacgcaatcc agtgattcca ttcaaggaca aaccggctgg ctaacatgcc cattccccag
     2161 ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg caatgcttca
     2221 gaaaatttcg gtttatggag ggtggcacct ttaccgattg atgcagcaaa tgcatccatc
     2281 gacataccaa acgcaagaag aacagtagca gtgatattca tgacaacgtc ctgaccgggg
     2341 tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac aagccgcgat
     2401 gggttttcgc gttcatgcag gcatagcgat tgatgtgcat atggtctcgc ctgactgaaa
     2461 gtcttcagtc cgtacgtgcc acgttttgca acgagtatgt tgacacgtac atttccggaa
     2521 tctggaaatt ggctactccc caaatgaggg cgcaacctta acatattttg agaatataaa
     2581 acaactgttg gaatatatta ttttattgct ttgt
//
LOCUS       DAZKFZ010000354         2612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000354 DAZKFZ010000000
VERSION     DAZKFZ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(286..543)
                     /locus_tag="ACHOQW_004809"
     CDS             complement(286..543)
                     /locus_tag="ACHOQW_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575708.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(540..1037)
                     /locus_tag="ACHOQW_004810"
     CDS             complement(540..1037)
                     /locus_tag="ACHOQW_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9575709.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGAHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELHHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPGLIAEINSLANQLQISI
                     PGLPG"
     gene            complement(1240..1677)
                     /locus_tag="ACHOQW_004811"
     CDS             complement(1240..1677)
                     /locus_tag="ACHOQW_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9575710.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1674..2171)
                     /gene="rrrD"
                     /locus_tag="ACHOQW_004812"
     CDS             complement(1674..2171)
                     /gene="rrrD"
                     /locus_tag="ACHOQW_004812"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP9575711.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKIDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2171..2386)
                     /locus_tag="ACHOQW_004813"
     CDS             complement(2171..2386)
                     /locus_tag="ACHOQW_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9575712.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(2463..>2612)
                     /locus_tag="ACHOQW_004814"
     CDS             complement(2463..>2612)
                     /locus_tag="ACHOQW_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575713.1"
                     /translation="VDAILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPE
                     SVMML"
BASE COUNT          596 a          701 c          618 g          697 t
ORIGIN      
        1 gtttcttcca ccatcgcacc ggacgggcga ccatgagggg agaacgccgc gctccgttta
       61 cgcggtaaac cccggtgtgt atcgtttttg attatccccg cacactcgcg cagaaggaat
      121 tccccgtcgg gctacggtca tggttaatgc ggaaatacag cgacgataca gcgcatgatg
      181 tgtcaggctt gaataccttt atccgttaaa agggatatca gttaagttat cccgtgtagg
      241 gtataagcca ttatcaagcc caccagtaga tgggctttgt aatggctact tcgcttttgc
      301 ttccgctcgc ttacgccggc gctcttcttt cctctcggct tttgccatgt ccatgaatgc
      361 ctgcatgatc gagttccgca tcatgtagct aacaaagtga tgattgacac agccgttgag
      421 gcgcagctgc tcgccaaact catccaccga ggccaatgct tccatcatac ccttctcgcc
      481 tttcatgaac tctgagaagt cgcgccccgc tctggaggcg cattcaatga cacgatcact
      541 catcccggaa gcccggggat cgaaatctgc aactggttag ccagggagtt aatttcagcg
      601 atcaaaccag gtttcgtgta gcgccatgcc gcgaggcctt gtccacagaa gctcgccatg
      661 tcttttttct gatcaaactc atgacatttc atgttgagct gcgcacttaa gctgttccga
      721 tgatgaagct ctccggtgaa gtaatcatcg aggactttat aggctgcata tttaaatccg
      781 gggtttagcc atgctgcgta atcataagca acgaactttc cgccataagt tccaccgtgc
      841 gcaccgcgtt ccgtaaaaac cacagattcg tggtttttct ccagctcagc caagaactct
      901 ttggtctgct tgtttcgcag gtagtggtac ggagattcag attcactttt accactggct
      961 ttccacatat cagtgaggca gatcatgcca tcttcaccga tacggattgg ttgattgaag
     1021 agggttaatg atttcatagc gtgtacctac tctttgaaat gaacctttgc cgcacaggaa
     1081 accagcccac cgaggctcgc cagcactaac tggtatcctc aaaggcccat tccaaagggg
     1141 caggttcggt gtaaaaaaca tgcgttgcgg tacgcattta ttgcaaaaag ccccgcatcg
     1201 cgaggctcat taaatggact ttgtgatttg caaaaaaaat tatttcaggc attgcgtcct
     1261 gatgtactcc tgcaggtagt taacctgcgc ggttatcttg tcgattccac ctcggagacg
     1321 gtaataattg agttcagcat ctgctgtaag tcctgggctt tctccatcgc ccatgctgct
     1381 ggctccggtc gttgactttg cacaggtggc ggagacttgc aggcgcttac gcccagcaga
     1441 aacatcagca cggagacttt cgatagtcgc attagcatca gcaagctcct ttgtgtatct
     1501 ggcgtcaagt tctgctacat cacgttgccg cttctgcata tcagcgatga tggatgtggc
     1561 tttatcgcgc tgctctttgt aggcgatggc gttatcacgg taatgattaa cagcccatga
     1621 aaggcagacg atgaggcaga tgaccagaac gtagataatc gcggttactc tgctcattgt
     1681 tgcccccaca aacagacttc acgctcaatc tcacggcgag tcatcagccc tttccattgc
     1741 ttaccgccag cgtatgtcca gcgccgtagc tgatcacatg cgcctttgat atcgccctgg
     1801 tttattttgc gaagaagcgt cgatgttctg aaattgccag cgcccacgtt gtaaacgaac
     1861 gagtaaagag cgccgcgcgt tgtttccggt atatcgattt tgatgtacgg gttaatttgt
     1921 ctggcgaccg tggcaaggtc tttattcagg agggctttgc attctgcttc ggtatacgtt
     1981 ttaccgggca tgatgtcttt tccggtgtgt ccgtgacata cagtccatac accaacaata
     2041 tctttatatg gtatgtagct gacaccttcc agaccatcgt taccacttgg gccagtaatt
     2101 aacactgatg ctatagcaat tgctccgcca ccaatagcag cagcaacggc ttttcgtaat
     2161 gatggaggca ttattcacct ctcgcagcct tgcgcttatc ttctttaatc ttgaaataaa
     2221 ggtttgtcag gtacgtcagc aggccaaaca gcagactccc cagcacacct attgccaccc
     2281 actgggacgg agagactttg tccagcagct gcagtaacca gtatcccgtc cccaccgctg
     2341 acgtggtgta tgacacacct gttgtgattt tttccatctg gtacataccc cgtctcccgt
     2401 tatccggaag ctcacaacat gaaaaaggcc agcagctgtt tactgatggc cctgactccc
     2461 cgttacagca tcatgaccga ttcgggttga ggttcagtcg catcggcgac cggtgattca
     2521 ggctgaactt caccgctctc tgcggtggta tctcccgctt cagtcggtgg ctctgcctgt
     2581 acaccaagca gctcatccag aatggcatca ac
//
LOCUS       DAZKFZ010000355         2598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000355 DAZKFZ010000000
VERSION     DAZKFZ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(323..601)
                     /locus_tag="ACHOQW_004815"
     CDS             complement(323..601)
                     /locus_tag="ACHOQW_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575714.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(768..1490)
                     /locus_tag="ACHOQW_004816"
     CDS             complement(768..1490)
                     /locus_tag="ACHOQW_004816"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9575715.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            1589..2488
                     /locus_tag="ACHOQW_004817"
     CDS             1589..2488
                     /locus_tag="ACHOQW_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9575716.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
BASE COUNT          695 a          590 c          574 g          739 t
ORIGIN      
        1 taaatatgaa ttcgacgatt taaccatttc tgtctgattg gtaatcatct gtgattaatg
       61 ctggtctatt gattactcca gcattaaccg cttagggatg tattcttcct gttgatatac
      121 agccctcgaa aaagagagaa aataaaactg tgtccaatat tactctacca ctccagaccg
      181 aagcatcggt agaattagca gaacgactgg tacaatagat tgggatagcg tcatttgtca
      241 ggtattccat tgcctgacaa atgacaattt tgagtcaata gacgtgcttc ttgatcatgt
      301 cttaaaaatt ataccatgag ccttatgaag catcattttt actgactggt gttaactttg
      361 ctatcatata aactctgttc ttaaaaaacg cgcccagaat tttatatgat ttcccttgtt
      421 tttctgcctg taaagaaatt tctttttcag tgctgttaat ggttgaaccg acggcagtaa
      481 tcgttttggc gacgctactg aatgataata aagacagtgc aaccacaaca attaaagaac
      541 agatagtatt cacgctaatt tccctgacaa ttgagaggat gagttttata tataaagaca
      601 ttaaacatga gtcatgttta cttcctgtga aaaataatct ttctgtaatt aaaccttcat
      661 ctccttcggg gtgagcaata cataatgtat cttgctatga aaaacacagg gatatataaa
      721 tattccttct gacctattta ataaaacgtg aatgtaaaag ttattcttca gtttgcagat
      781 gcagtgggtc tgatggtaat ttcggttgta tcaacgcttt cgggagattc aataatatgc
      841 cttacagcac gggcaatatc cgcgggttga agtgcaacag aacggtaggc gtccattgcc
      901 ttcatggttt ccacatgggt aatggttgaa gccagttcac tttcgacaac gccaggattt
      961 acgcaggtca cacggatttt cgagctttcc tgccgtaagc catcagaaat agcccgtact
     1021 gcaaattttg atgcacaata gactgcgcct gtgggcacaa cagaaagggc accaatagaa
     1081 ccaagattga ttatctgacc ggaaccctgt gcttccatca ccggaagtac agcgccaatt
     1141 ccccacagta cacctttgat attcacgtca atagtgagcg cccattcatc ttgttttcct
     1201 gctgcaagcg gtgaaagcgg cataacgccc gcattattga taagaacatc aactcgcccc
     1261 cagctatcca gcgctgcttg cacgaaatcg gccatggact gtcggtctgt gacatccaac
     1321 tcccgcgctt tagcaattcc tcctgcgcgg cagatttcgg ttgcaatggc ttcgatacgc
     1381 tcaactctgc gtgctcccag taaaaccttt gcacctgtca ttccaagctc tctggcaata
     1441 ccttccccaa taccacttga tgcgccggta attaatatga ccttatccat tacgttctcc
     1501 ttcaaatgtg ttcggagaga gaatagaact ttatgattgg ttccagtatc ctgctttcaa
     1561 tgtactcagt ggttagcggc attaaacaat gaaaatcgat cttaatcttt tacccatttt
     1621 tatcgcggta gcagaagaga gtaattttag taaagccgcc gcacggctgg gcgttacccg
     1681 ttcagcggtc agtcagggaa taagacgcct tgaagatgca ttcgggacaa tgcttgtaat
     1741 gcggaccacg cgctcagtta atttgacaga agcgggagaa cggctgcata aatcgctgtc
     1801 attaccttta tccggtattg aggcagcgtt cgaagagatg gtttctgacc atgtgccacg
     1861 cggacttctc agaatcgcgg tgacgtcaat agctgaagaa tttctttcag gtccgctaat
     1921 cgcttcgttt gcagcagcca atccagccgt tacgcttgat attgttgtca cggatgagga
     1981 attcgatatc gtggccgcag gttacgatgc tggcgtaagg ctcggagagg tcattgaaaa
     2041 agacatgatt gctgtgcccc ttaccggcag gcagcgtgaa atggtcgttg cctctccctc
     2101 ctatctcgca gccaacagca cacctgttca tccgcgtgaa cttgtgaatc ataagtgtat
     2161 cggctggcgt cagtccccgg aggttgcgcc ttatcgctgg ccatttgaag aaaacgggag
     2221 agctttcgac cttgcgattg aaccgcagat tacgactaat gatctgcgtc tgatgttgag
     2281 gctggctctt gccggcggag gaataacaat tgccactcag gaaactttca ggccgtatat
     2341 tgaaagcggt aagcttgtat ctctgcttga tgactttctt ccacaatttc cgggcttcta
     2401 tctgtatttc ccacagcgtc gcaatattgc accaaagctc cgcgctctga ttgaccacgt
     2461 taaggaatgg cggcagcaat tggcttaaat gtctgcacct gcattgcctg atgtcagaac
     2521 agtattttga ttaattgcca gggttacaat ggcacaaatg cggcacagga ggaaacgtgg
     2581 tgtatttaaa tatggggt
//
LOCUS       DAZKFZ010000356         2581 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000356 DAZKFZ010000000
VERSION     DAZKFZ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            72..2351
                     /gene="ydeP"
                     /locus_tag="ACHOQW_004818"
     CDS             72..2351
                     /gene="ydeP"
                     /locus_tag="ACHOQW_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP9575717.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAQNTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSVEFLARLGERYGFTPPHVPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          672 a          611 c          642 g          656 t
ORIGIN      
        1 atccctttgt atgtactgtg catcatcgct attacaaatc ctaataattc atttccacac
       61 aggataagta gatgaagaaa aaaattgaat cctaccaggg tgctgccggt ggttggggtg
      121 ctgttaaatc cgtagcgaat gcagtacgta agcagatgga tatacgccag gatgttattg
      181 ccatgtttga catgaataag ccagagggct ttgactgtcc gggttgtgca tggccagatc
      241 ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc gcctgggaag
      301 tcacggataa gcaggtaaat gcctctttct ttgctcagaa tacggttcaa tcattactta
      361 cctggggaga tcacgagctt gaggctgcgg gacgtctcac tcagcctttg aaatatgatg
      421 ctgtcagcga ctgttacaag ccattaagct ggcaacaagc tttcgacgaa attggcgcac
      481 gccttcaaag ctatagtgat cccaatcagg ttgaattcta tacttcgggc cgcacttcca
      541 atgaagctgc ctttctttat cagctttttg cccgtgaata cgggagcaat aactttcccg
      601 actgctccaa catgtgccat gaaccgacaa gcgtgggttt ggcagcgagt atcggtgtag
      661 gtaaagggac cgtgttgctg gaagactttg agaagtgcga tttagtcatt tgcattgggc
      721 ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta gtgaaacggg
      781 gagcgaaaat gatcgccatc aatcctctac aggaacgtgg cctggagcga tttaccgcac
      841 cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca gttggccagt gcctactata
      901 acgtgcgcat tggtggtgat atggcgttgc tcaaggggat gatgcgcctg ttaattgagc
      961 gcgatgatgc tgcaagcgcc gcaggtcggc cctcattgct ggatgacgaa tttattcaaa
     1021 cgcataccgt cggctttgac gagctacgcc gtgacgtgct caattccgag tggaaagata
     1081 tcgaacgtat ttctggacta agtcagacac aaatcgccga actggctgat gcatatgccg
     1141 ctgccgaacg aaccattatc tgttacggaa tggggatcac tcagcacgaa catggtaccc
     1201 agaatgtaca gcaactggtc aatctgctgt tgatgaaagg taacattggc aagcctggtg
     1261 cgggtatctg cccactacgt ggccactcta atgtacaggg cgaccgaacc gtcggtatca
     1321 ccgagaaacc gtctgtagag tttctggctc gcctgggtga gcgctatggc ttcaccccac
     1381 ctcatgtacc tggacatgct gcaattgcca gcatgcaagc aatatgtacg gggcaggctc
     1441 gagcattgat ctgcatgggg ggcaactttg cactggcaat gccagatcgg gaagcgagcg
     1501 ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc cactaagctt aaccgctctc
     1561 atctgctgac cgcacggcat agctatattc tgccggttct gggacgtagc gagattgaca
     1621 tgcaaaaaag cggtgcgcag gcggtaaccg ttgaggattc aatgtcgatg attcatgcct
     1681 cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgct gtggtcgcgg
     1741 gaatcgcgca ggcaacacta ccccagagcg tggtagcctg ggagtatctg gtggaagatt
     1801 atgatcgcat tcgcaatgac attgaagctg tgctgccaga gttcgccgac tataaccaac
     1861 gcatccgtca tcccggtggt tttcacctga taaatgcagc tgctgaaagg cgctggatga
     1921 cgccgtcagg taaggctaat ttcattacca gcaaagggct gttagaagat ccctcttcag
     1981 cgtttaacag taagctggtc atggcgacag tacgcagcca cgatcagtac aacacgacga
     2041 tttatggtat ggatgatcgc tatcgagggg tattcggtca acgagatgtg gtctttatga
     2101 gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga aagagttaat cttattgcgc
     2161 ttacgccaga cggtaagcgc agctcacgcc gcatggatag attaaaagtg gtcatttacc
     2221 ctatggctga ccgctcactg gtgacctatt ttccagaatc gaatcacatg ctaacacttg
     2281 ataaccacga tccattaagt ggcattcctg gctataaaag tattccggtt gaattagaac
     2341 catcaaatta atgcctcttc tcatttcttc tgctgtcatc cgcacagcag aagaattcct
     2401 cattgattat tatcgcgcaa tttgctcaca tggattaaat taaactacat actataagat
     2461 ataaacttct gcctacagct gtaagaaact ccgctcagta ctgaagcacc agtcctattt
     2521 cctcttttct caagcctgtt atattaagca tactgattaa cgatttttaa cgttatccgc
     2581 t
//
LOCUS       DAZKFZ010000357         2458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000357 DAZKFZ010000000
VERSION     DAZKFZ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            125..328
                     /locus_tag="ACHOQW_004819"
     CDS             125..328
                     /locus_tag="ACHOQW_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9575718.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1375
                     /locus_tag="ACHOQW_004820"
                     /pseudo
     CDS             328..1375
                     /locus_tag="ACHOQW_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1403..1492)
                     /locus_tag="ACHOQW_004821"
     tRNA            complement(1403..1492)
                     /locus_tag="ACHOQW_004821"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ser,seq:cga)
     gene            1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHOQW_004822"
     CDS             1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHOQW_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP9575719.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
BASE COUNT          728 a          519 c          577 g          634 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa
     1381 tgatgtgtaa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     1441 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     1501 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     1561 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1621 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1681 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1741 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1801 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1861 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1921 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1981 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2041 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2101 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2161 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2221 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     2281 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     2341 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     2401 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaa
//
LOCUS       DAZKFZ010000358         2452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000358 DAZKFZ010000000
VERSION     DAZKFZ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(148..1152)
                     /gene="argF"
                     /locus_tag="ACHOQW_004823"
     CDS             complement(148..1152)
                     /gene="argF"
                     /locus_tag="ACHOQW_004823"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9575720.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAMMVATLSK
                     "
     gene            1314..1730
                     /gene="rraB"
                     /locus_tag="ACHOQW_004824"
     CDS             1314..1730
                     /gene="rraB"
                     /locus_tag="ACHOQW_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP9575721.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1776..2279)
                     /locus_tag="ACHOQW_004825"
     CDS             complement(1776..2279)
                     /locus_tag="ACHOQW_004825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9575722.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          646 a          597 c          567 g          642 t
ORIGIN      
        1 ccaacagcgt ttaaatacac taaatcctcc ggaaaattta cgtatagcaa tagaaaaatt
       61 tggctggaag aaaaaaacta tcactgcata aaataatttg acgccgggat gtttctgtat
      121 ttcccggcat ctttatagcg atatcaatta tttactgagt gtcgcgacca tcatcgcttt
      181 gatggtgtgc atgcggtttt ccgcctgatc aaaaacaatg ctggcggcag attcgaagac
      241 ctcatcagtc acttccattc cgccatgtag gccaaactct tccgccattt tcttgccaag
      301 cgtcgtttgg tcatcatgga acgcgggcag gcagtggagg aatttgacct ccgggttacc
      361 ggtcaactgc atcatcttgc tgttcacctg ataatcacgc agtaatgcaa tgcgttcagc
      421 ccatttttct tttgcttcac ccatcgacac ccatacatcg gtatagataa agtcagcacc
      481 ttcaacgcct ttagctacat cttccgtcag cgtaatattc ccgccatttt gttgtgccag
      541 ggcgcggcat tccgtaacca gcgcagcttc cggccagcat gcctgtggcg cgaccagacg
      601 caaatccaga ccggtaagcg ccgcagcttc gagcatcgaa ttgcccatgt tgttacgtgc
      661 gtcacctgca tagaccagcg tcatttcgtt gaatggttta ccgggtaaat gctcctgcat
      721 ggtgaggaga tccgccagca actgcgtggg gtgaaactca ttggtcaggc cgttccatac
      781 cggcacgcca gcgtattccg ccagtgtttc gacaatctcc tgaccatagc cgcgatactg
      841 aataccgtca tacatgcgac ccagcacgcg ggcagtgtct ttaatcgact ctttatgacc
      901 aatctgactg ccgcttgggc cgagataagt aacgcgagca ccctggtcat atgcggcaac
      961 ttcgaaagag catcgggtac gggtcgagtc tttttcgaag atgagcgcga tgtttttacc
     1021 agtgagtttg gcttcttctt tgccgctttt cttatcggct ttcagcttcg cggctaactg
     1081 cagcaggctg ttgagttcag ctggcgtgaa atcgagtaat ttcaggaaat gcttatgata
     1141 aaacccggac atagatccct cctgtggcta acgtctcaat gaattaaaat tcaatttata
     1201 tggatgatta ttcatttgca agtctaaagc ataaatcttt gtcacaaagg tggaggcaat
     1261 gccagtggtg tgtgacaata tgagtatcgg caggacatta agaggaatga gccatggcaa
     1321 acccggaaca actggaagaa cagcgtgaag aaacacgttt gattattgaa gaattactgg
     1381 aagatggcag cgatccggac gcgctgtaca ccatcgaaca ccatctttcc gcagacgatc
     1441 tggaaaccct ggaaaaagca gcagttgaag cgtttaaact cggttacgaa gtgaccgatc
     1501 cagaagagct ggaagttgaa gatggtgata tcgtgatttg ctgcgacatc ctcagcgagt
     1561 gcgcgttgaa tgccgatctg atcgatgccc aggttgaaca actgatgacg ctggcggaga
     1621 aatttgacgt tgagtacgac ggttggggca cttactttga agatcccaac ggcgaagacg
     1681 gcgacgatga agattttgtc gatgaagacg atgacggggt tcgccactaa ttaattgatt
     1741 aaaatgcccg atagcacaca ctagcgggca tttttttaga gttcgcgtag catccgcact
     1801 tcacaatcaa catggcccgt gcagccaagt gcatagtcga tatgttgaaa acctaaatgt
     1861 tcatacaaac caatcgcttc tttcaaaaaa gcggtcgttt ccagatagca gcgtttgaag
     1921 cccatttctc ttgcctcctc catcgccttt aaggccagtt tttttgccag ccctttgccg
     1981 cgaatagccg gaagaaaata catcttttgc agttcgcaaa tgtccgattc actacctgct
     2041 aatggcgcaa tcccaccgcc gccgaccact tcaccctcgt actcaaccac ccaatatgca
     2101 tgacccggct gactatacac ttgatacagc tcgtcaagat tcggatcggc gacggtgtag
     2161 cctttatcag cggtaagacc gtattcggcg gatacctgac gaatgacgcg ggcgatagca
     2221 ggattatcct gcagcgtcag gcgacgcatt acaggtgatt gcggcgcaat gttattcata
     2281 gtttaaccca ataaaaataa agtaattata catttgttta ataccactct cttcttacgt
     2341 gcgcaagcgt cttataaagc aataaagttt ttgccagcct attactgata agagtattgt
     2401 aacgtggcag aacattttac tgtctgtatc tataaattat atcaatggat tt
//
LOCUS       DAZKFZ010000359         2438 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000359 DAZKFZ010000000
VERSION     DAZKFZ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1345
                     /locus_tag="ACHOQW_004826"
     CDS             <1..1345
                     /locus_tag="ACHOQW_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFP9575723.1"
                     /translation="PGVEKFIKDFNDAKKKGEHAYDMTLSYQNFDKGFFNSRFQMQMT
                     FDNGAPDLNIKPGQKVVFDVDVEHGPLPITMLMHGNVIPALAAAKVNLVNNELTQPLF
                     IAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGKFSFGEGQFTFNGDSSSLSNLDIEG
                     KVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVGESESKFNQINIINHGEDVAQID
                     AFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQ
                     YNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELN
                     ANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKQAD
                     KQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1386..2318)
                     /locus_tag="ACHOQW_004827"
     CDS             complement(1386..2318)
                     /locus_tag="ACHOQW_004827"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP9575724.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          768 a          517 c          528 g          625 t
ORIGIN      
        1 gcctggtgtc gaaaaattta ttaaagattt taacgatgct aaaaagaaag gtgaacatgc
       61 ctacgatatg acgttaagtt atcaaaattt tgacaaaggc ttttttaatt cccgttttca
      121 aatgcaaatg acattcgata acggtgcacc cgatctcaat atcaagccag gccagaaagt
      181 tgtatttgat gtggatgttg agcacggtcc gttgcccatc acaatgttaa tgcatggtaa
      241 tgtcatccca gcactggcag cggcaaaagt gaacttagtg aataatgaac tgacacaacc
      301 gctatttatc gccgcgaaaa ataaatcgcc cgtggaagcg acattgcgat tcgcgtttgg
      361 tggctcattc tctacgacat tagatgttgc ccctgcagag tatggaaagt tttcttttgg
      421 tgagggccag tttactttta atggtgatag tagttcattg tctaacctgg atattgaagg
      481 caaagtcgaa gatattgttc tgcaattatc accaatgaac aaagtaacgg caaaaagttt
      541 taccattgat tctctggcgc gattagaaga aaagaaattt ccggttggtg aaagcgagtc
      601 gaaatttaat cagattaaca ttatcaatca cggggaagac gttgcccaaa tcgatgcttt
      661 cgttgcaaaa accaggctgg atcgcgttaa agacaaagat tatatcaatg tcaatctgac
      721 ctacgaactt gataagttaa caaaagggaa tcagcaactc ggtagtggtg agtggtcatt
      781 gattgctgaa tctattgatc cctcagcggt gcgccaattt atcatccagt ataacattgc
      841 gatgcagaag cagcttgctg cacaccctga attagcaaac gatgaagttg ctctgcaaga
      901 agtgaatgct gcattgttca aagagtattt accgttatta caacaaagtg agccgaccat
      961 taaacaaccg gtaagatgga agaacgcact cggcgaacta aatgccaatc tggatatcag
     1021 tattgccgac ccagccaaat cttcatcatc cacaaacaaa gatatcaaat cgctcaattt
     1081 tgatgtgaag ttaccgctta atgtcgtcac agaaaccgca aaacagctta atttatctga
     1141 aggaatggat gcggaaaaag cgcaaaagca ggctgataaa caaatcagcg ggatgatgac
     1201 attaggtcag atgtttcagt taatcacgat tgacaacaat accgcctcgc tgcagctgcg
     1261 ttatacaccg ggtaaagttg tttttaacgg acaggagatg agcgaagaag aatttatgtc
     1321 tcgtgccgga cgttttgttc attaaaatac tcactcgcct ctgctcattc agaggcgagc
     1381 aaaatttact tgtcctgccg ctgtgatgac atcaacgacg tcagaagtcg gtctttttcc
     1441 tgccacaatg agttcagcca ttgctgaaaa tggcgcttaa agcttttgtc attgatgtag
     1501 tcgccatgta actcatcggc aataggctgt aaatccacat gtacgacaat ccgcgtcagt
     1561 ttaccgctta acatatcgaa gaacggctga cggttattgt ccggataaca caacgtaaca
     1621 ttaagcagtt tatcgaattg tttacccagt acatttagcg ccatcgcaat gcctgccgcc
     1681 tttggtggca acaagttttg gaaagtggag tgggtttgct gatgtttttc ttgcgtgaaa
     1741 cgggagcctt caacgaaatt gacaatggtg gtgggatgca ggcgaaactt ttcacaagaa
     1801 cgccgagtgg tttcaacatc tttaccacgt cgctccggat gacgtaataa ataagcgcgg
     1861 gaataacgct tcataaacgg catatccagc gcccagcacg ccaggccaag aaatggcacc
     1921 caggccagct gttgcttgag gaaatattta ttcatcggaa tgtgcttacg aaacagcacg
     1981 cacagtacga caatatctgc ccaactacgg tggttacaaa taagcagata ccagttcttc
     2041 ttacttaacc cttccagccc gtgaacttcc cattgcaggt gtgggttcag atgcagtaat
     2101 accgccagac cttcacacca gcaatacatc ataaaatcac aaaaacgcga aacctttcgc
     2161 caaatgactg gcacaggtag caatagtttt acaatcccgg caatgatgat cgggacggaa
     2221 caaaaaatag tgaccaaaat ggtcaatacg atactcagca aaagggttat cgcagcgagt
     2281 attctcgtca taatgaattt attcaaaagg ttagccatag acagtgcgct aaagaagcgc
     2341 aaggcgcaga ttttagcaga aaacgaccca aataacggat gatccttaag gagaaaaata
     2401 attcatatct atccacatta gaaaaaatcc cattatct
//
LOCUS       DAZKFZ010000360         2431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000360 DAZKFZ010000000
VERSION     DAZKFZ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            337..531
                     /locus_tag="ACHOQW_004828"
     CDS             337..531
                     /locus_tag="ACHOQW_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575725.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGAWL"
     gene            528..2408
                     /locus_tag="ACHOQW_004829"
     CDS             528..2408
                     /locus_tag="ACHOQW_004829"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9575726.1"
                     /translation="MKTWFSIKAMADVVYVRIYDEIGGYGVKASALTDEINACGNASE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPENIKNMISQKTGSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYGTRSQIVNA
                     AFNHSSSDFTYILAGGAEKSVLAGWEQSGETFRQWTKKGSLSNFHEARRVGLNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLNQLTVIPQAMGRAAAR
                     TVGNLVHLVLTGNTKLSDGIPLFSKKHGNDIDNGVSTTGLGIARRAMRTQRDENGEVL
                     NIVPKFLLVPAALEDRALQMVNSTTPFGADTNSGSYNPYHKQLDIIVDPRLDEVSDKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVRAI
                     SS"
BASE COUNT          704 a          545 c          620 g          562 t
ORIGIN      
        1 tataagctat tgatatgttg agaaataaaa atttttagtg tcgaatcagg ttgttttttg
       61 ttcatcaccg gaacgttccc gaaaacattt acaaaaaaca ggcgcaaaaa aagcgccccc
      121 gattgctgtt accggaggcg cttttacacg acaaaggagt ttttatcgcc aggatgacga
      181 gtcttaatac tgcttcaatg gcaaaaatgc gtcaataact ttgcctttct gagaataatc
      241 agaaaaatca taatctgatt ttcaggtaga aaatgattta tctattactt ttatcgatca
      301 ataatgatgc ccgttaatca aaacggaggc ggatttatgc cagagaacaa caccagaaag
      361 ccggataaaa gtgccacggt acacatagac gccggaacta tggagaagat cgaacgctat
      421 cagcagttca tcaaagataa tcacccgggt atgccagtac ccacgaaagg acaaatcaca
      481 cgcagcgcgg ttgaatactg gtacagggca acgttagggg cctggctatg aaaacatggt
      541 tttccattaa ggctatggca gatgttgtct atgtgcgcat ttatgacgag atcggcgggt
      601 acggtgtaaa agcatcggca cttactgacg agatcaacgc gtgtggtaat gcgtctgaaa
      661 tccatcttcg catccattca cctggtggcg acatctttga agggctggct atctataacg
      721 ccctgaaaaa tcatccggca aagaaaattg tacacattga gggcatggcg gcttctatgg
      781 cctcgtttat tgccatgtgt ggcgatcaca tcgttatgcc tgaaaacgcg atgatgatga
      841 tacatgcccc ccgtggtgtt actgccggag tgtcgggcga cgttcgccgc tttgctgacc
      901 tgatggacaa gctgggcgac acgatggcag aaacctacgc cggaagaacg ggcaggagta
      961 aacaggaaat caccgccatg atggaggcgg aaacctggat ggatggcaat gagtgtaagg
     1021 ctaacggttt cgcagatgag gttatacccg cgattacagc aatggcccga attgaatcaa
     1081 aacgaatcgg agatttttca aatatgccgg aaaatattaa aaacatgatc agccagaaaa
     1141 ctggcagcgg agaacaggaa cgactcaacg ggatacgtga attgtttggc acgttcaacg
     1201 gaagatataa cgacctggct ataagttgtc ttgctgattc agaatgtagc gttgagaatg
     1261 cacgcgaacg ccttttactc gctatgggta aagaatcaac gccaacaaac aaaaccaccc
     1321 ccgcaaatct ttactacgcg tacacggata acggcaacat aaccggcgat gcaatgcgcc
     1381 aggggcttaa tgcgcgtctt ggtcacgaac gggccgaacg cggtaatcct tacgccatga
     1441 tgagcctttt cgatatggca caggcatcat taacccatcg tggtataagc acgggcagct
     1501 acggcacacg ctcgcagata gtaaacgcgg cattcaacca cagcagcagc gattttactt
     1561 atatccttgc tggtggcgct gaaaaatccg tgcttgccgg atgggagcaa agcggcgaga
     1621 catttcgcca gtggacgaaa aaaggttcat tgtcaaactt ccatgaagcg cgtcgtgttg
     1681 gtctgaatgg cttctcgaca ttaaacaaag tgccggaagg tgcggaatat aaatacataa
     1741 ccaccagtga ccgtggtgaa ccaatcgcgc tggccaccta cggtaatatt ttcagcatta
     1801 cccgacaggc gattattaac gatgatctga atcagttaac cgttatcccg caggcaatgg
     1861 ggcgagcagc cgccagaacg gtgggtaatc tggttcatct ggtgctgaca ggaaacacaa
     1921 agctttctga cggtataccg ctgttcagca aaaaacacgg aaatgatatt gataacggag
     1981 tcagcactac agggcttggc attgcacgcc gtgcaatgcg cacacagagg gatgaaaacg
     2041 gtgaggtgct gaatattgtg ccaaaattcc ttcttgttcc agcggctctg gaagacagag
     2101 cgttgcaaat ggttaactca accacgcctt ttggtgccga tacaaacagt ggttcatata
     2161 acccgtacca caaacaactg gatattattg tcgatccccg ccttgatgaa gtcagtgata
     2221 agcaatggta catgctttca gcacagggaa cggacacaat tgaggtggct tatcttgacg
     2281 gtaatgacga gccttacctt gagcagcagg agggctttat tgttgacggt gtggcctgga
     2341 aggtccgcat tgatgctggc gttgctgctc tggattatcg cggaatggtc agagcgattt
     2401 cttcatgaga acaaggcggc agcagccgcc t
//
LOCUS       DAZKFZ010000361         2429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000361 DAZKFZ010000000
VERSION     DAZKFZ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1661)
                     /locus_tag="ACHOQW_004830"
     CDS             complement(<1..1661)
                     /locus_tag="ACHOQW_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9575727.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDL"
     gene            complement(1636..2181)
                     /locus_tag="ACHOQW_004831"
     CDS             complement(1636..2181)
                     /locus_tag="ACHOQW_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9575728.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
BASE COUNT          563 a          687 c          585 g          594 t
ORIGIN      
        1 agatcaaaaa tatccgggtt attcgggaag tgaacggcac cgggaagcgg ttcatcccct
       61 tccggcgtca gtgtgaagcg gttataaatc tgctctttcg cggtatccgt accgatttcg
      121 gtaaggtaaa ccccgttttt gtttcgctta cgtggcatgc tggccaccgg ctttccgtag
      181 acggatgccc ctttaatggg gatcacccgg aacagcccat gttttttcga gcgttcatac
      241 acaatggtcg ggtcaatccc gccagtatcc cagcagatac gggataccga catttctgca
      301 ccattccggc gggtataggt tttattgatg gcctcatcca cacgcagcag cgtctgttca
      361 tcgtcgtggc ggcccataat aatctgccgg tcaatcagcc agctttcctc acccggcccc
      421 catccccata cgcgcatttc gtagcggtcc agctgggagt cgataccggc ggtcaggtaa
      481 gccacacgat caggaacggg cgctgaataa tactctttcc gctctgccat cacttcagca
      541 tccggacgtt cgccgatttt cgcttcccac gtctcaccga gcgtggtgtt cacgaaggtt
      601 ttacgttttc ccgtatcccc tttcgtcttc atccagtctt tgacaatctg cacccaggtg
      661 gtgaacgggc tgtacgccgt ccagatgtga aaggtcacac tgtcaggcgg ttcaatctct
      721 tcaccggatg acgaaaacca gagaatgcca tcacgggtcc agatcccggt cttttcgcag
      781 atataacggg catcagtaaa gtccagctcc tgctggcgga tgacgcaggc attatgctcg
      841 cagagataaa acacgctgga ggggtcatcc ggcgtccatt tgaggccaaa cggcgtctct
      901 ttgtcgccaa atttaagata ctgctcctcc ccgcagtgcg ggcaggcaac atgaaaacgc
      961 ataaaatgcg gggattcact ggctgcacgc tcaatctggc aggtgcctct cactttgggc
     1021 gtggagccac ggatggactt tggccagacc gagccttcaa tacgcttgtc acccaggaac
     1081 gtcggagagc cttcctgttc aatatcctca tcaaaggcag caagttcatc ataacccgcc
     1141 acatcaaccg acttttcacg gtagtttttt gccgctttac cgcccaggca ccagaagcca
     1201 cgaccattgg tgaaacgctt catagtgagc gtgttatccc ggtgcttttt gccataccac
     1261 ggggccagag acagcagcga cggaatatcg cggatggtag gctcgacgtg ggttttcata
     1321 aagttctcgg catcaccatc cgtcggcaac cagataaggg tgttacgctg cttatgctct
     1381 atgaagtagg cataaacacc cagcagcatt tttgaataac caacacgggc agacttcacc
     1441 acattcacct cgcggatgta gtcgctgccc atcgcattca tgatagcccg ctgaaagggt
     1501 agtgtttccc agcgcccttc ctggtatgcg gattctttcg ggagatagta actggcatcc
     1561 gcccattcaa cggcagtctg tggctccggc ctgaacaggg ctcgcagccc ggcgcgtaca
     1621 tcacgccgca gaatatcaat ctgactgttc gatatattca ctcagcaacc ccggtatcag
     1681 ttcatccagc gcggctgctt tgttcatggc tttgataata tcccgtttca ggaaatcaac
     1741 atgtcggttt tccagttccg gaaaacgccg ctgtactgag agagggatcc cgtcaagaat
     1801 actggcaatt tcacctgcga tccgtgacag cacgaaagta cagaatgcgg tttccaccac
     1861 ttctgcggag tctctggcat tcttcagctc ctgggcgtca gcctgcgcac gcgtaagtcg
     1921 atggcgttcg tactcaatag tcccaggctg gagatctgcc tcgctggcag ccctgtaatc
     1981 ctcaacctct ttacggagtt tttcattttc gatatcagct tccctctgcg cataccactg
     2041 aattgccgtg gcggtatcaa atacagattc aacgccctta ccacctccgg agacgcaagg
     2101 gagcccctga gactgccagc gttcaatcgt tcgcggatcc acgttgaaaa tttcggcaag
     2161 tttcttttta ttaaccttca tgaaacagtc tcacaacaaa tacagggtcc gacatgaaag
     2221 tgcccgaaaa tgactttttc aggcgttttc atgtcggacc tttacggatt cgatattaga
     2281 aaaaacaaat agttatgttc gagaagtacc gacatgattt tccccggaaa attttcataa
     2341 atagcgaaaa cccgcgaggt cgccgccccg taaccggtcg gatcgccgga aaggacccgc
     2401 aaaatgataa taattatcat ctacatgtc
//
LOCUS       DAZKFZ010000362         2362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000362 DAZKFZ010000000
VERSION     DAZKFZ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(113..748)
                     /gene="msrQ"
                     /locus_tag="ACHOQW_004832"
     CDS             complement(113..748)
                     /gene="msrQ"
                     /locus_tag="ACHOQW_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP9575729.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(749..1753)
                     /gene="msrP"
                     /locus_tag="ACHOQW_004833"
     CDS             complement(749..1753)
                     /gene="msrP"
                     /locus_tag="ACHOQW_004833"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP9575730.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1861..2274)
                     /gene="hiuH"
                     /locus_tag="ACHOQW_004834"
     CDS             complement(1861..2274)
                     /gene="hiuH"
                     /locus_tag="ACHOQW_004834"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP9575731.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNKHYHVPLLLSQYGYSTYRGS"
     gene            2296..>2362
                     /locus_tag="ACHOQW_004835"
     CDS             2296..>2362
                     /locus_tag="ACHOQW_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575732.1"
                     /translation="MSFEDSEKASRVTLQQHYNFVM"
BASE COUNT          612 a          564 c          581 g          605 t
ORIGIN      
        1 aatttgaggt tgatgacaaa cgatacataa cctgatgtga tctgcgtagg ccggataggg
       61 cgctcgcgcc acatccggca gggataacgc gtactttgtc ggcaatatga agttatacag
      121 ataatttgtt gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcgac ttcttatacc
      181 gtaaggctaa aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct
      241 tcactgacca cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca
      301 actgttgcca gtgcttgccg agttttcgct gcatcgcctg ggttgaagta aacgctaaag
      361 caagcaaaat aatccagctg ataattccta acgttaaata aggccgggta attaactcct
      421 tgcctaataa cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca
      481 gtgtcgccca ggcaaagcac cataagccta acaggcggcg tgtgcgtatc aataacggct
      541 gttttgcgta gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag
      601 tgcgaccggt aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga
      661 tcgcccagac cagccagaga aacggcaaca atccggcaag atgcaggcaa actttcagcc
      721 atgtcacctg ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat
      781 acagcgatgc cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgtacat
      841 cgaggatgcc gcctgaacca ataaatcgtt cggtagcctg tgaccagcgt gggtgatcaa
      901 catgcggatt aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg
      961 ttggcggacg ctcgcgggtc agcttaatac tgacgatcga tttaatccct ttaaagccat
     1021 atttccacgg cacaatcagt cgcaccggcg cgccattttg tggcggtaac gccttgccat
     1081 aaacacctac ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga
     1141 cataaggata tttcagcccg ccgccgataa agcggtcctg ctggcctggc atctgttccg
     1201 gtgcataaat tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca
     1261 gcaatttgtg cagcggaaaa ccaatccacg gcaccaccat cgaccacgct tccacgcagc
     1321 gcatacgata aatacgctct tccagcggga agcgacgggt taaatcatcg tgatcgaggg
     1381 tcaatggttt tgccacttcg ccgctgattt tcagtgtcca tggatcggtt ttcaggctac
     1441 cagcattagc ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg
     1501 agactttatc tgctggcgtc agtggcaggt tattttgcca ggcggcaggc ttgctgaact
     1561 ccagcgcttt tccggcgggg gcgggtgggc gatcgttccc tttaaaccag ctaagcagat
     1621 cggcatgcgc agcgtgaggc aaagaaagtg cagctgcgct gatgcccagt gcttttaaca
     1681 cctgccgacg cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt
     1741 gattcttttt cataagccat cacacttttt gtcgttagcc agcaagagcc gtttactgtt
     1801 aatctgtcag ggagtgtcct ccctaacagt agcagagatc tgcttttaca tatttatgca
     1861 ttaactgcca cgataggttg aatacccata ttggctaagt aataaaggca catgataatg
     1921 cttgttcact ttattaatat gaaattcaac ggggatctca gggaagaaac tttcaagatt
     1981 ttgtttcttg aaatagtccc cggttttaaa tacgacacgg taatcgcccg tagttgcagt
     2041 ttgctcgggc cacagtgcct taattcgtcc atccttatct gttttggcgg tattaagttg
     2101 taaccagccg ttgtccgctt tcttttcaag agtgactgtc acgtcggcag cgggttttcc
     2161 tgtttgctgg ttcaaaatgt gcacgctaag aatgttttgt tgtgcagcat agaccaaaga
     2221 aggtaatgaa aatgcagccg ttactacgga gagtactaaa taacgcttta acatgtttat
     2281 atccttgtca tgtgaatgag ttttgaggat agcgaaaaag cgtccagggt aaccttacag
     2341 cagcattaca attttgtaat ga
//
LOCUS       DAZKFZ010000363         2356 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000363 DAZKFZ010000000
VERSION     DAZKFZ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            162..512
                     /locus_tag="ACHOQW_004836"
                     /pseudo
     CDS             162..512
                     /locus_tag="ACHOQW_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            549..830
                     /locus_tag="ACHOQW_004837"
     CDS             549..830
                     /locus_tag="ACHOQW_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFP9575733.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(878..1051)
                     /locus_tag="ACHOQW_004838"
     CDS             complement(878..1051)
                     /locus_tag="ACHOQW_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575734.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(1248..1781)
                     /locus_tag="ACHOQW_004839"
     CDS             complement(1248..1781)
                     /locus_tag="ACHOQW_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575735.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            complement(1928..2032)
                     /locus_tag="ACHOQW_004840"
                     /pseudo
     CDS             complement(1928..2032)
                     /locus_tag="ACHOQW_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT          735 a          533 c          468 g          620 t
ORIGIN      
        1 tgaaaaatgg ctgaattaac caaatggcta caaaacacga ttaccggaat tgaaacggta
       61 gtagacgata aatcgtttgt atgtgatgaa atagtattca aaatcgatgt ggttaaaaac
      121 gtacttaccg catttaaagt cgcgctggta tcgctggaag tattgatgat actttgtcga
      181 tcagcgatgc tctctcagaa ctaaaccatt tcattaaccg gcacgcagac aatacgaaat
      241 atttaaaagt ctggggtaac ggggccacct tcgacaacgt aattttacgt ggagcttacg
      301 agcgagcagg acaaatctgc ccgtgggcgt actggaatga ccacgatgta cgcacgatcg
      361 ttacgcttgg gcgttccatc ggattcgacc ccaaaatgga catgcctttc gatggcgaac
      421 ggcacaacgc cctggctgat gcccgtcatc aggcaaaata tgtttccgct atctggcaga
      481 aattaattcc tgccaccagc acagaattat gattttcccg ggtgcagccg gttttgatgg
      541 agaaaattat gaacaccttg tttttactga tggctgaatt caatacccca aacattgaac
      601 tctcagcagt tagccaaaag tactttggta tgagtccagc cacggcagaa gcaaaagcaa
      661 acgcttgtaa gttgcccgtt ccaacatatc gcatcggcac atcacaaaaa gcaaaacgtt
      721 gcatcaatat tcaagatctt gcggaataca tagacaaaag gcgagaagaa ggacgtatcg
      781 agtgggaaca ggtcagaaca gtcaaacaga aggacaaaga acatcactaa agaaaaaacc
      841 cgcctgaagg cgggttttca aaaagcacca gctatgatca tgctgctttg cgacgacgaa
      901 gcttaccctg ctgctcttta ccagagacag tagcgtgagt gaacgcatta ggagcagcct
      961 tcatcagaac ttcaacagca gcacccatac ctgcgaatgc tttcattgtg tcgaacttaa
     1021 cctgtggctt ggttgctttt tgatctttca tagaaaactc ccgagacagt aaaggcgtct
     1081 ctaacccttt ctttaaagct agcttgtttc gctaacttat gccaatcgat catgtcgatt
     1141 ggtgacatcg tttcttagta gtttaagcac aaaacgactg ccatagatgt acctttaagg
     1201 taatctggac gggtatccta caatttgtag acccttctcg tctataccta ctgagcaaat
     1261 ttaagaaaga tatcctgcag ctcatcaatg actgccgaca tcacataacc gcactgttcc
     1321 atgcggaaac caaaagactc gtaatactgc accagttctg gtactggctc tacaatgtgg
     1381 acaactttac attcaacagc tttacaaaat ataaaagcac tcataagagt gagtaaaacc
     1441 atgcgccctt tcaatgggtg agattcatct tctctagaaa acctttcgat catatggata
     1501 cgaaagatgt tttcttcaac cccataaaca caaattgctg ctcctgatgg tattccctga
     1561 acccgacctt gctgaacaag ttttatgcag aactcatact tttctctgga gttgccatag
     1621 gtacttaacg catagtccca ttcaagctca ccatagccac cacacagaat cttgtaatca
     1681 tcatcactga gcggaccaac agcaagaggt aagccgacat gatcaataat caactggata
     1741 ttgttacgta ctgattgacc tatctcgtcc agggtaagca tcatggcctc tcaagcggaa
     1801 cactaaaagt cgcattatat ctcattctta agccgcgtat ggattacacc ttgaaatgaa
     1861 aacgccgggt tcccaatagg ctcccacaaa gtatataact atttgttttt caaaaacggt
     1921 acatcctatc gagcattggt gcaacgctaa accgaccact ccagtgaacg tcagtttttt
     1981 caggcattgc gctggtttgg ttgatttttt gcatttcaga attaccgtgc attttcaaat
     2041 gtagagatta ttttatcgat atatcatgtg gttatgttat tcagcatcac tgttcaggag
     2101 gctcaataac ggggtactat accataacaa caggaagcgc ctgtctcatt gcaaaagaaa
     2161 attgagatcc tctcaaggca tgaagctctc acgaagtgaa tatctaatct tattagatgt
     2221 tagcgttgtg aaaagcaatg ataaacaata cagattcgac gataaataaa aatcacacat
     2281 taaactccgg tgatatctct ccctgctaat agcactgata gagaaaaaaa gaacccaata
     2341 aacattgggt tctttt
//
LOCUS       DAZKFZ010000364         2335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000364 DAZKFZ010000000
VERSION     DAZKFZ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..206)
                     /locus_tag="ACHOQW_004841"
     CDS             complement(<1..206)
                     /locus_tag="ACHOQW_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9575736.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDA"
     gene            complement(190..513)
                     /locus_tag="ACHOQW_004842"
     CDS             complement(190..513)
                     /locus_tag="ACHOQW_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575737.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            638..1114
                     /gene="racR"
                     /locus_tag="ACHOQW_004843"
                     /gene_synonym="rarC"
     CDS             638..1114
                     /gene="racR"
                     /locus_tag="ACHOQW_004843"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFP9575738.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1430..1582
                     /locus_tag="ACHOQW_004844"
     CDS             1430..1582
                     /locus_tag="ACHOQW_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9575739.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(1697..2212)
                     /locus_tag="ACHOQW_004845"
     CDS             complement(1697..2212)
                     /locus_tag="ACHOQW_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575740.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
BASE COUNT          671 a          552 c          497 g          615 t
ORIGIN      
        1 gcatcagggg tgtctttatc cagccaacgg aaaattttct gggtattacg agccagggct
       61 tccggatggc tgtcgtcgta cagttccggg aacgtcatcc ccagttcgaa ataagtccgg
      121 gctatttcag ctgcaggaac tttctcacca tcaggatatg cccaggcatt catcgccatg
      181 cggatgtgct catgtttgat tttcatgaat catttgcctc ttgatgcttc gggtatgatc
      241 gttttcgtca tttggttgct tcatcgacat attctgcgaa taacatgacg agcgtcgtaa
      301 gtatgtccaa tcaacatcag gacgaagttc ttcacacagg acaccacctt ttgttgctcg
      361 ttcaatcgca ggacatctct cagcaggcaa ctgacgtaca cctttgatcc attgatttac
      421 gcttggagga gatacaccta aaagcctagc cattgctgat tgcccaccga caacagcaca
      481 agctcgtttg aatgaatagt tatctttttt catcgaatga actccaaaaa acacacaata
      541 atattaggct tagcctaatg caattgtcaa taggctatgc ctaacgcttc gagagtaggt
      601 attgcctaat gcgatgcgca taggagacta ttaagcaatg cttagtggta aagacttagg
      661 ccgagcgata gagcaggcca ttaacaaaaa aattgcatca ggagccgtca aatcaaaggc
      721 ggaaatcgca cgtcatttca aagtccaacc accatcaatc catgactgga ttaagaaagg
      781 ttcgataagt aaagacaaac ttccagaact atggcgtttc ttttctgatg tggttggtcc
      841 agagcattgg gggcttaacg aataccccat accaacccca tccacttcag atacaaaaag
      901 tgaactttta gacataaaca gcctttatca agccgcctct gatgaaaaaa gagcaattgt
      961 ggctttcctc ttatctggaa atgctacgga gcctagttgg gttgatcatg acgttcgtgc
     1021 ctacattgcc gcaatggaaa tgaaggtagc taactatctg aaaaatcaag aatcaaaacg
     1081 gaaaagccag aacatcacca agacaggaac ttaaacttat atggtccgac gggaaattcc
     1141 tagttcccgt tagttaactc ctactacctc tcccacaaac catcacctat taggtcgcac
     1201 ccaaatcatt aggcgtagcc tattgacaag caattaggca tttcctatag ttttcccata
     1261 ccaacccatc ccgtcccaca caatacaggg caatacctag agttacccgg cagtggtcag
     1321 ggattaagta gccagcccga ggcgtaagaa catgacggca gggttcaact ttaactatgc
     1381 agcaggtttt tgttccgcta ccccggcgtt aaggggatat gaggtcagca tggatactct
     1441 tgatcttggc aacaacgaat ctctggtgtg cggcgtgttt cccaaccagg acggcacgtt
     1501 caccgcgatg acgtatacca aaagcaaaac gtttaaaacc gaatctggcg cgcgtcgctg
     1561 gttagcaaga aactctgact aatgaaatct ggtaattaag gaatcctcca cggggggaga
     1621 gtggtgcaca cgcgccggac acaagcaagc atccggcatg ctctttaaca atctggatat
     1681 ccataacagt aaaaatctac agattgccgt tcagttttct ggccaactct tcaatagatg
     1741 gaggtgatac gtaatccgga tttttattca taagaaactg attttcacag tacaggcacc
     1801 tacttttatg aaaaaattca tattctctaa ccgggaatgg ctgaagtatc gatactatct
     1861 tttgtccaaa acattttgga caaagatgca tgattatgct gccgccgttc acggttacct
     1921 ccttcgagta cacaaaagtt cccgactcaa gttggctaag gatataacct tccgtctgag
     1981 cctcaaagtt ttcaaattct gcaattttag ctttgagaga agcatttatt tcttgataag
     2041 tgcccaccag ctcaacgaga gacacgcatt cgcgctgaat agatgcaagc tttgagttca
     2101 gttcaccaat agccgcattt acttcagctt gagtttttgc ctcgttcatt agttttgcaa
     2161 tctgggcggt ttcacgaata gccgtcattg ctgccgttaa ttcagcgatc acattcaata
     2221 ctcttattgt tgttggggat atccagatta acaagatcct tgttgttggg gaataacggg
     2281 gaccacctcg cctgacgtgg taaaaagcag gcacacaaca cgaaagcaca cggcg
//
LOCUS       DAZKFZ010000365         2279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000365 DAZKFZ010000000
VERSION     DAZKFZ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            23..166
                     /gene="kil"
                     /locus_tag="ACHOQW_004846"
     CDS             23..166
                     /gene="kil"
                     /locus_tag="ACHOQW_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP9575741.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            241..537
                     /locus_tag="ACHOQW_004847"
     CDS             241..537
                     /locus_tag="ACHOQW_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9575742.1"
                     /translation="MNAYYIQDRLEAQSWTRHYQQIAREEKEAELADDMGKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            543..1328
                     /gene="bet"
                     /locus_tag="ACHOQW_004848"
     CDS             543..1328
                     /gene="bet"
                     /locus_tag="ACHOQW_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9575743.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKVLGFLKQKASEQKVAA"
     gene            1325..2005
                     /locus_tag="ACHOQW_004849"
     CDS             1325..2005
                     /locus_tag="ACHOQW_004849"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9575744.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2002..2184
                     /locus_tag="ACHOQW_004850"
     CDS             2002..2184
                     /locus_tag="ACHOQW_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9575745.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            2157..>2279
                     /locus_tag="ACHOQW_004851"
     CDS             2157..>2279
                     /locus_tag="ACHOQW_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9575746.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLA"
BASE COUNT          614 a          557 c          594 g          514 t
ORIGIN      
        1 aatcagccag gagtcccaaa aaatggatca aacacttatg gctatccaga ctaaattcac
       61 tatcgccact tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa
      121 atggatatta atactgaaac tgagatcaag caaaagcatt cactaacccc ctttcctgtt
      181 ttcctaatca gcccggcatt tcgcgggcga tattttcaca gctatttcag gagttcagcc
      241 atgaacgctt attacattca ggatcgtctt gaggctcaga gctggacgcg tcactaccag
      301 cagatcgccc gtgaagagaa agaggcagaa ctggcagacg acatgggaaa aggtctgccc
      361 cagcacctgt ttgaatcact ctgcatcgat catttgcaac gccacggggc cagcaaaaaa
      421 gccattaccc gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc
      481 cggtacatgg ttgaaaccat tgctcaccac caggttgata ttgattcaga ggtataaagc
      541 ggatgagtac agcactcgca acgctggcag ggaagctggc tgaacgtgtc ggcatggatt
      601 ctgtcgaccc acaggaactg atcaccactc ttcgccagac ggcatttaaa ggtgatgcca
      661 gcgatgcgca gttcatcgca ttgttgatcg tcgccaacca gtacggcctt aatccgtgga
      721 cgaaagaaat ttacgccttc cctgataagc agaacggcat cgttccggtg gtgggcgttg
      781 atggctggtc ccgcatcatc aatgaaaacc agcagtttga tggcatggac tttgagcagg
      841 acaatgaatc ctgtacatgc cggatttacc gcaaagaccg taatcatccg atctgcgtta
      901 ccgagtggat ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca
      961 ccggcccgtg gcagtcgcat cccaaacgga tgttacggca taaagccatg attcagtgtg
     1021 cccgtctggc cttcggattt gctggtatct atgacaagga tgaagccgag cgcattgtcg
     1081 aaaatactgc atacactgca gaacgccagc cggaacgcga catcactccg gttaacgatg
     1141 aaaccatgca ggagattaac actctgctga tcgccctgga taaaacatgg gatgacgact
     1201 tattgccgct ctgttcccag atatttcgcc gcgacattcg cgcatcgtca gaactgacac
     1261 aggccgaagc agtgaaagtt cttggattcc tgaaacagaa agcctctgag cagaaggtgg
     1321 ctgcatgaca ccggacatta tcctgcagcg taccggaatc gacgtgagag ctgtcgaaca
     1381 gggggatgat gcatggcaca aattacggct cggcgtcatc accgcttcag aagttcacaa
     1441 cgtgatagca aagccccgct caggaaagaa gtggcctgac atgaaaatgt cctacttcca
     1501 caccctgctt gccgaggttt gcaccggtgt ggctccggaa gttaacgcta aagcactggc
     1561 ctggggaaaa cagtacgaga acgacgccag aaccctgttt gagttcactt ccggcgtgaa
     1621 tgttattgaa tccccgatca tctatcgcga cgaaagtatg cgtaccgcct gctctcccga
     1681 tggtttatgc agtgacggca atggccttga gctgaaatgc ccgtttacct cccgggattt
     1741 catgaagttc cggctcggtg gtttcgaggc cataaagtcg gcttacatgg cccaggtgca
     1801 gtacagcatg tgggtgacac gaaaagatgc ctggtacttt gccaactatg acccacgtat
     1861 gaagcgtgaa ggactgcatt atgtcgtggt tgagcgggat gaaaagtaca tggcgagttt
     1921 tgacgagatg gtgccggagt tcatcgaaaa aatggacgag gcactggctg aaattggttt
     1981 tgtatttggg gagcaatggc gatgaagcat cctcacgata atatccgggt aggtgcgatc
     2041 actttcgtct actccgttac aaagcgaggc tgggtatttc ccggcctttc tgttatcaga
     2101 aatcccctga aagcacagcg gctggctgag gagataaata ataaacgggg agctgtatgc
     2161 acaaagcatc tcccgttgag ttaagaacga gtatcgggat ggcacatagc ctcgctcaaa
     2221 ttggagtcag gtttgtgcca ataccagtag aaacagacga agaatttcat acgttagcc
//
LOCUS       DAZKFZ010000366         2262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000366 DAZKFZ010000000
VERSION     DAZKFZ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1421
                     /gene="mppA"
                     /locus_tag="ACHOQW_004852"
     CDS             <1..1421
                     /gene="mppA"
                     /locus_tag="ACHOQW_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP9575747.1"
                     /translation="FEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNAKWADGTP
                     VTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTAVDVHTLK
                     IQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERVVNEKLVV
                     VPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKLLKDIPGQ
                     VYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPAWHFTPDV
                     TAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQKIAIAVA
                     SMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLLTSTHSGN
                     ISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNGRLIKPWL
                     KGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1472..>2262)
                     /gene="ynaI"
                     /locus_tag="ACHOQW_004853"
     CDS             complement(1472..>2262)
                     /gene="ynaI"
                     /locus_tag="ACHOQW_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP9575748.1"
                     /translation="CTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKIT
                     IIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGD
                     WIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTI
                     GLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVW
                     AEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          659 a          518 c          543 g          542 t
ORIGIN      
        1 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
       61 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
      121 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
      181 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
      241 cgattattga tggtaaagct acacctgacc agcttggcgt caccgcagtt gatgtccata
      301 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
      361 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
      421 atctgatcgg caatggcgct tatgttctta aagagcgcgt agtcaatgaa aaactggtcg
      481 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
      541 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
      601 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
      661 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
      721 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
      781 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
      841 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
      901 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
      961 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     1021 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     1081 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     1141 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     1201 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     1261 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     1321 ataccaatgg acgattaatc aaaccgtggc tgaaaggtta tcccattaat aaccctgagg
     1381 atgtggcgta cagtcggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     1441 acccttgtgg ataacgccag tgatgtctga tttagcgacc ctgttccggc ggtgtaatgt
     1501 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgtacaatat
     1561 cgataatctt caaataaacg tcttgctgtg cagcaagcca ctcagcccat accgtggttt
     1621 tggtaaagca ataaaccata atattcaatg acgagtcagc aaactggttg aaataaacca
     1681 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     1741 cgataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     1801 ggttggtcat tcgtcctggg ttttctacgc tgatcgatga aaacagcgag ttcggtacgt
     1861 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     1921 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     1981 aatagagcat gatcccggaa aagaaattac tcagaatatc tttgccagcc ataccgacag
     2041 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     2101 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     2161 cactggtgat atcgcgaccc tttttaatct gctgtttttc aaactgatta atcagcaaaa
     2221 atagcttaat cgtcagaata accgcaatca gggacgtaca ga
//
LOCUS       DAZKFZ010000367         2250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000367 DAZKFZ010000000
VERSION     DAZKFZ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(60..269)
                     /gene="fumD"
                     /locus_tag="ACHOQW_004854"
     CDS             complement(60..269)
                     /gene="fumD"
                     /locus_tag="ACHOQW_004854"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP9575749.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            624..818
                     /locus_tag="ACHOQW_004855"
     CDS             624..818
                     /locus_tag="ACHOQW_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575750.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            826..2238
                     /gene="pykF"
                     /locus_tag="ACHOQW_004856"
     CDS             826..2238
                     /gene="pykF"
                     /locus_tag="ACHOQW_004856"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP9575751.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          614 a          560 c          497 g          579 t
ORIGIN      
        1 actggaccgc aactgaagca agaatatcag gatgaagctt atcgcctcat cctgaatggt
       61 cattttacca gtgcgttaat gacggtttcc atcgcctgct tttccagctc actgcgctga
      121 atgcgtttat tggctaatgc tgtacgtaat acgcctgcga taacaatatg ctttggttcc
      181 tgccccagat cgcgcatttc cagcacaact ttaccgacaa ctctgcacat ttctcggtac
      241 agttcatcct cttttgtccg atttcccatt ctttatacct atttatcatg ccaactatca
      301 gcatatatca atctaaccaa ttaaacaaaa atcaaacaaa atcagacaaa taacgcgata
      361 aattatttta actgtagcaa ttgagcgatg atgtatttat acaccggatg aactttcact
      421 tatcttcaca gtgacaaccc cggcaccaga cgttgcgcaa acagcgaagt ttttgcgtaa
      481 ccttttccct ggaacgttaa atctttgata acaatttatt gtctaacaag ttgtatattt
      541 tttgaaacgc tgtttttgtt ttccttttgg attaatttca gcgtataatg cgcgccaatt
      601 gactcttgaa tggtttcagc actttggact gtagaactca acgactcaaa aacaggcact
      661 cacgttgggc tgagacacaa gcacacattc ctctgcacgc tttttcgatg tcacctatcc
      721 ttagagcgag gcaccaccac tttcgtaata ccggattcgc tttccggcag tgcgcccaga
      781 aagcaagttt ctcccatcct tctcaactta aagactaaga ctgtcatgaa aaagaccaaa
      841 attgtttgca ccatcggacc gaaaaccgaa tctgaagaga tgttagctaa aatgctggac
      901 gctggcatga acgttatgcg tctgaacttc tctcatggtg actatgcaga acacggtcag
      961 cgcattcaga atctgcgcaa cgtgatgagc aaaactggta aaaccgccgc tatcctgctt
     1021 gataccaaag gtccggaaat ccgcaccatg aaactggaag gcggtaacga cgtttctctg
     1081 aaagctggtc agacctttac tttcactact gataaatctg ttatcggcaa cagcgaaatg
     1141 gttgcggtaa cgtatgaagg tttcacaact gacctgtctg ttggcaacac cgtactggtt
     1201 gacgatggtc tgatcggtat ggaagttact gccattgaag gtaacaaagt tatctgtaaa
     1261 gtgctgaaca acggcgacct gggtgaaaac aaaggtgtga acctgcctgg cgtttccatt
     1321 gccctgccag cactggctga aaaagacaaa caggacctga tctttggttg cgaacaaggc
     1381 gtagactttg tcgctgcttc ctttattcgt aagcgttctg acgttatcga aatccgtgag
     1441 cacctgaaag cgcacggcgg cgaaaacatc cacatcatct ccaaaatcga aaaccaggaa
     1501 ggcctcaaca acttcgacga aatcctcgaa gcctctgacg gcatcatggt tgcgcgtggc
     1561 gacctgggtg tagaaatccc ggtagaagaa gttatcttcg cccagaagat gatgatcgaa
     1621 aaatgtatcc gtgcacgtaa agtcgttatc actgcgaccc agatgctgga ttccatgatc
     1681 aaaaacccac gcccgactcg cgcagaagcc ggtgacgttg caaacgccat cctcgacggt
     1741 actgacgcag tgatgctgtc tggtgaatcc gcaaaaggta aatacccgct ggaagcggtt
     1801 tctatcatgg cgaccatctg cgaacgtacc gaccgcgtga tgaacagccg tctcgagttc
     1861 aacaatgaca accgtaaact gcgcattacc gaagcggtat gccgtggtgc cgttgaaact
     1921 gctgaaaaac tggatgctcc gctgatcgtg gttgctaccc agggcggtaa atctgctcgc
     1981 gcagtacgta aatacttccc ggatgccacc atcctggcac tgaccaccaa cgaaaaaacg
     2041 gctcatcagt tggtactgag caaaggcgtt gtgccgcagc tggttaaaga gatcacttct
     2101 actgatgatt tctaccgtct gggtaaagaa ctggctctgc agagcggtct ggcacacaaa
     2161 ggtgacgttg tagttatggt ttctggtgca ctggtaccga gcggcactac taacaccgca
     2221 tctgttcacg tcctgtaata ttgcttttgt
//
LOCUS       DAZKFZ010000368         2208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000368 DAZKFZ010000000
VERSION     DAZKFZ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1833
                     /locus_tag="ACHOQW_004857"
     CDS             <1..1833
                     /locus_tag="ACHOQW_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9575752.1"
                     /translation="TLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQH
                     VPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGV
                     RFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTF
                     RINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLG
                     TGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKL
                     HLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLEDQSAIIQQIQRVQKDT
                     NDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTT
                     PLFVAQGNQLFMNDVFLKRLFAVSITSSANPPTFSLTPEGRLTARNADISGNVNANSG
                     TLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQP
                     FDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMP
                     AGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGISIS"
     gene            1901..>2208
                     /locus_tag="ACHOQW_004858"
     CDS             1901..>2208
                     /locus_tag="ACHOQW_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9575753.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     P"
BASE COUNT          526 a          558 c          653 g          471 t
ORIGIN      
        1 accctgcctg atggtgtgga gacatacggt gtatggggac tatccctgcc gtcactgcgc
       61 cgtcgcctgt tccgctgtgt ctccgtccgg gaaaacacgg acggcacctt tgccatcacg
      121 gcggtgcagc acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg
      181 cagtcaggca ccctgaacag cgtcatccct ccggcagtgc agcacctgac ggtggaggtg
      241 agcgcagctg acggccagta tctggcgcag gtgaaatggg acacgccgcg ggtggtgaag
      301 ggcgtgcgct tcagtctgcg cctgaccagc ggaagcggag aaggcagccg tctggtgacc
      361 accgccatca ctgcggatac agagcatcgt tccagtggtc tgccgccggg ggaatacacc
      421 ctgacggtca gggcgattaa cagttatggc cagcaggggg aaccggccac caccacgttc
      481 aggattaatg cacctgcggt acccgccacg attgagctga caccgggcta ttttcagata
      541 acagcggtcc cgcgtcttgc ggtgtatgac ccgacggtac agtttgagtt ctggttttcg
      601 gagacaaaaa tcgcagatac atctcaggtg gaaacctctg cccgttatct ggggaccggc
      661 agtcagtgga gtgtatccgg cccgcacatt aagcccggga aggatttctg gttttacgtg
      721 cgcagcgtca acctggtggg gaaatctgct tttgtggaag ccagtggccg ggccagcaat
      781 gatgcagaag ggtatctggg gctgtttcgg gaaaaaatag gaaaactgca tctggctcag
      841 gggctgtggg agctgataga caacagccag cttgcggatg agatggcgga gatgaagacc
      901 tccatcaccg aaacccgcaa tgaaatcaca cagacggtca gtaaaacgct ggaggaccag
      961 agcgccatca tacagcagat acagcgcgtg cagaaggaca caaatgatga ccttgctgca
     1021 ctttacatgc tgaaggtaca gaaaacaaaa aatggcatac cctatgttgc cggtattgga
     1081 gcggggattg aggatactga tggccagccc ctgagcaaca tactgctgct ggctgaccgt
     1141 attgcgatga ttaacccgga ggacggcaac accacgccgt tatttgtggc gcaggggaat
     1201 cagttgttca tgaacgatgt gttcctgaaa cgactgtttg cggtgagtat cacgtcatcc
     1261 gccaatcccc cgacgttttc cctgacgccg gagggcaggc tgaccgcaag aaatgctgat
     1321 atcagcggta acgtgaatgc gaattccggg acgctcaaca acgtcacgat taacgagaac
     1381 tgtcgggttc tgggaaaact gtccgcgaac cagattgaag gcgatctcgt taaaacagtg
     1441 ggcaaagctt tcccccggga ttcccgtgca ccggagcggt ggccatcagg gaccattacc
     1501 gtcagggttt atgacgatca gccgtttgac cggcagattg ttattccggc ggtggcattc
     1561 agcggcgcta aacatgagag agagcatact gatatttact cctcatgccg tctgatagtg
     1621 cggaaaaacg gtgctgaaat ttataaccgt accgcgctgg ataatacgct gatttacagt
     1681 ggcgttattg atatgcctgc cggtcacggt cacatgacgc tggagttttc ggtgtcagca
     1741 tggctggtga ataactggta tcccacagca agtatcagcg atttgctggt tgtggtgatg
     1801 aagaaagcca ccgcaggcat cagtatcagc tgaattttat aacccatata cgggcgccag
     1861 aaatggcgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaac tttatgccgc
     1921 cattttgtcc gcagccattt gtctggccgt atccggtgcg cctgcatggg cgtctgaaca
     1981 tcagtcaacg ctgagcgcgg ggtatcttca tgtctcgacg aacgttcctg gcagcgatga
     2041 actgaacggg attaacgtga aataccgtta tgagtttacg gacacactgg ggatggtgac
     2101 gtcgttcagc tatgcaggag acaagaatcg ccagctgacc cgttacagcg atacccgctg
     2161 gcatgaagat tccgttcgta accgctggtt cagcgtaatg gcggggcc
//
LOCUS       DAZKFZ010000369         2185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000369 DAZKFZ010000000
VERSION     DAZKFZ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.372"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..245
                     /locus_tag="ACHOQW_004859"
     CDS             <1..245
                     /locus_tag="ACHOQW_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9575754.1"
                     /translation="SEITSLVTAEAVKEVLRSEEVRSALKRKLRHNLEARLDAEVDAI
                     LDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            323..538
                     /locus_tag="ACHOQW_004860"
     CDS             323..538
                     /locus_tag="ACHOQW_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9575755.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            542..1099
                     /locus_tag="ACHOQW_004861"
     CDS             542..1099
                     /locus_tag="ACHOQW_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575756.1"
                     /translation="MNHEEMNQRFSCLENEIAELNKKLSALMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNCSELEWECNRPSFVVSGDDGKITISENGKVTLPSHQ
                     HSETLIEFAIDYLKNNKKQGLMQRIGRCMGYLQVAAEIEALASGADKDAIVREALLRN
                     FNTPPFKKVPAYWLHPGLTYLKVRI"
     gene            1136..1669
                     /locus_tag="ACHOQW_004862"
     CDS             1136..1669
                     /locus_tag="ACHOQW_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9575757.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1968..>2185
                     /locus_tag="ACHOQW_004863"
     CDS             1968..>2185
                     /locus_tag="ACHOQW_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFP9575758.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELA"
BASE COUNT          564 a          464 c          641 g          516 t
ORIGIN      
        1 tgtcagaaat tacatccctg gtcactgctg aagcagtgaa ggaagtcctg cgctctgaag
       61 aagtccggag cgcactgaaa cggaaacttc gccataacct ggaagcgcgt cttgatgcag
      121 aagtggatgc cattctggat gaactgctgg gcgcaccggc agctccggag ccggaaggca
      181 tcgcgggtga ggggagtgct tcagatagcg gtgaccccac accggacagc gacatgatga
      241 tgtaagcatg cgtcagggac catcggtgtg tgccggtggt cttttttatt gttgtgagct
      301 tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacag gcgtgtcata
      361 caccacgtca gcggtgggaa cgggctactg gttcctgcag ttgctggaca gggtttcccc
      421 gtctcagtgg gcggcaatag gcgtgctggg gagtctgctg tttgggctgc tgacatatct
      481 gacgaacctg tatttcaaaa tcagagagga ccggcgtaag gcggcacggg gagagtaggt
      541 gatgaaccat gaagaaatga atcagcgctt cagttgtctg gaaaatgaaa ttgctgaact
      601 gaataaaaaa ctgtcggcgc tgatgccttc tgaagatgaa aaaaaacgcc gcgatgagca
      661 gtttgctgcg tttgacgatt attgtcggaa agtgatgagc agaaatctcg cagagtgttt
      721 cagtattcat aatgataatt gcagtgagct ggaatgggag tgtaaccggc catcctttgt
      781 tgtatccggt gatgacggga aaatcaccat atcagaaaat ggaaaagtaa cactgccatc
      841 gcaccagcac agtgagacgc tcattgaatt tgccattgat tacctgaaga acaataaaaa
      901 acaggggctg atgcagcgca ttggtcgttg catggggtat ctgcaggtag ccgctgagat
      961 tgaagcgctg gccagtggtg ctgataagga tgcaattgtg cgggaggctc ttcttcgtaa
     1021 ttttaatact ccacccttta aaaaagtgcc ggcttactgg cttcatcccg gactgactta
     1081 tctgaaagtg cgtatttagt gggcctggga cagcggctga atatttaata tatccatgaa
     1141 cacaaaaatc aaatacggcc tgtcggctgc cgttctggcg ctgattgccg caggtgcttc
     1201 tgcgcctgaa attctcgacc agtttcttga cgaaaaagaa ggtaaccaca ccacggcata
     1261 ccgtgatggt gcgggtatct ggaccatctg ccgtggagcc acccgggtgg atggtaagcc
     1321 tgttattcct ggcatgaagc tgtcgaaggg gaaatgcgac cgggttaacg ccattgagcg
     1381 tgataaggcg ctggcatggg tggagaaaaa catcaaagtg ccactgactg aaccccagaa
     1441 agcggggatc gcgtcattct gcccgtataa cattggcccc ggtaagtgtt tcccgtcgac
     1501 gttttataaa cgaattaatg caggcgatcg caggggagcg tgtgaagcga ttcgctggtg
     1561 gattaaggac ggaggcagag actgccgtat ccgctcaaat aattgctatg gtcaggtatc
     1621 ccgtcgtgac caggagagcg cgctggcgtg ctggggtatc gacagataag cagaatattt
     1681 tgctgaaaaa tgacgttggc caacgcggac ggataacacg aaatcctgcg aactggcaaa
     1741 acctgagtga ataaaagtaa aaaccccgtt tgttagcagc aggtggggtt ttgtgtttcc
     1801 tgactccgga aaagtcaaag gagaaagtgt gtttgatttt agcaaactga ttcgggagat
     1861 ttgagtgatg gctgaaaaat tatctacctg gaagttcatt cttatctggc tggtgtttgt
     1921 gattatggtc tccggttatt tcatcggtca gatacgctgg tggtgaaatg aaccgcgtac
     1981 tgtgtgtggt catcattgcc ctgctggtgg cctgtggtgc gcttagtctg gggctgaatc
     2041 attaccgtga taacgccatc acctacaaag cgcagcgcga taaaaaagcc agagagctgg
     2101 agctggcaaa cgcaaccatt actgatatgc aggtgcgcca gcgcgatgtt gctgcgctcg
     2161 atgcaaaata ctcaagggaa ttagc
//
LOCUS       DAZKFZ010000370         2179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000370 DAZKFZ010000000
VERSION     DAZKFZ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.373"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            166..2151
                     /gene="yjcS"
                     /locus_tag="ACHOQW_004864"
     CDS             166..2151
                     /gene="yjcS"
                     /locus_tag="ACHOQW_004864"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP9575759.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          573 a          571 c          548 g          487 t
ORIGIN      
        1 gtcgtacttt tccctcttca gcacaaatgc ctaataaaat ggatcgttcc gctcacactt
       61 ccacttatta aagtcgcaat ttcccaatta tgataacgcc agctttaaat gacgtatttt
      121 gcatggatta catatttacg acataaatga taaatggaga tttatatgaa taactctcgg
      181 ttattccgtt tgagcaggat tgttattgcg ttaactgccg ccagcggcat gatggtaaat
      241 accgctaacg cgaaagagga agcgaaagcc gccactcaat atacccaaca ggttaatcag
      301 aattacgcca aatcattacc gtttagcgat cgtcaggatt ttgacgatgc ccagcgtgga
      361 tttatcgccc cgctgctgga tgaaggtatt ctgcgtgatg cgaacggtaa agtttactac
      421 cgcgcagacg attacaaatt tgatattaat gccgctgcgc cggaaaccgt aaaccccagc
      481 ctgtggcgtc agtcgcagat caacggcatt tctggcctgt tcaaagtcac cgataaaatg
      541 tatcaggtgc gcggccagga tatctctaac attacgttcg ttgaggggga gaaaggcatt
      601 attgtcatcg acccgctggt gacgccgcct gccgcaaaag ccgcacttga cctttacttc
      661 cagcatcgtc cgcaaaaacc gattgttgcc gttatctaca ctcacagcca caccgaccac
      721 tatggtggcg tgaaaggcat tatctctgaa gccgatgtta aatccggcaa agttcaggtg
      781 attgcccctg ctggctttat ggacgaagcc atcagcgaaa acgtgctggc gggtaacatc
      841 atgagccgcc gtgcgctcta ctcttacggt ctgttactgc cgcacaacgc gcaaggcaac
      901 gtgggtaatg gccttggcgt gacgctggca acgggcgacc cgagcattat tgcaccgact
      961 aaaactatcg tcagaactgg cgagaagatg attatcgacg gcctggagtt tgacttcctg
     1021 atgaccccag gtagcgaagc gccagccgaa atgcacttct atattccggc cctgaaagcc
     1081 ctgtgtaccg ccgagaacgc cacgcatacc ctgcacaact tctacactct gcgcggcgcg
     1141 aaaacccgcg ataccagcaa gtggaccgag tacctgaacg aaacgctgga tatgtggggt
     1201 aacgacgcgg aagtactgtt tatgccgcac acatggccgg tctggggcaa taagcatatc
     1261 aatgattata ttggtaaata tcgcgatact atcacgttca ttcacgacca gaccctgcac
     1321 ctggcgaacc agggctacac catgaatgaa atcggcgaca tgattaagct gccgcctgca
     1381 cttgccaata actgggccag ccgtggctat tacggttctg tcagccacaa cgcccgcgcg
     1441 gtgtataact tctatcttgg ctattacgac ggtaacccgg ctaacctgca tccgtatggt
     1501 caggtagaga tgggcaaacg ttacgtgaaa gcgctgggcg gctctgcccg tgtcatcaac
     1561 ctggcgcaag aagcgaataa gcaaggtgat taccgctggt cggcagaact actgaaacag
     1621 gtgattgccg ccaacccggg tgaccaggtg gcgaagaatc tgcaagcgaa taactttgaa
     1681 cagctgggct atcaggccga gtccgccaca tggcgcggtt tctacctgac cggcgcgaaa
     1741 gagctgcgcg aaggggtgca taagttcagc cacggcacca ccggttcccc ggacaccatt
     1801 cgcggaatgt cggtcgaaat gctgttcgac tttatggccg ttcgcctcga tagcgcgaaa
     1861 gctgcgggta aaaatatcag cctgaacttc aatatgagca acggcgataa cctcaacctg
     1921 acgctgaacg atagcgtgct taactaccgt aaaacactgc aaccgcaagc cgacgcctct
     1981 ttctacatca gccgtgaaga tctgcacgcc gtgctgaccg gacaggcaaa aatggcggat
     2041 ctggtgaaag cgaagaaagc caaaattatt ggcaatggcg cgaaactgga agaaattatc
     2101 gcctgtctgg ataatttcga tttgtgggtg aatatcgtaa ccccaaatta atgtcactcg
     2161 cttttggggg cgctgaagc
//
LOCUS       DAZKFZ010000371         2173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000371 DAZKFZ010000000
VERSION     DAZKFZ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..99
                     /locus_tag="ACHOQW_004865"
     CDS             <1..99
                     /locus_tag="ACHOQW_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9575760.1"
                     /translation="RRQLAAPKRPLGPTPIELLKQEYERRKAAGFV"
     gene            134..904
                     /locus_tag="ACHOQW_004866"
     CDS             134..904
                     /locus_tag="ACHOQW_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9575761.1"
                     /translation="MGTVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAGEGESGVTEEQPAQSEVPDVLTGEVEQKVTADMMIEFICQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGSLARVNQNGKFRYCIPGADLPAEPEAASVTETDGKA
                     FPQPTGVALPVREAETQEEIKTESVAVTVQSQPLFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKHRDIVRQIVDSSGRIVSEK"
     gene            920..1315
                     /locus_tag="ACHOQW_004867"
     CDS             920..1315
                     /locus_tag="ACHOQW_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9575762.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            1372..1728
                     /locus_tag="ACHOQW_004868"
     CDS             1372..1728
                     /locus_tag="ACHOQW_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575763.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERAYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRILRQEERERRRNIEKLIQIE
                     RNVKARIRRVTKSRTE"
     gene            1777..1989
                     /locus_tag="ACHOQW_004869"
     CDS             1777..1989
                     /locus_tag="ACHOQW_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575764.1"
                     /translation="MSNAQKVINAEKYNEWVKKFSEQIFKITGDENAAKNELEPWTPE
                     GVDPNYCWWDVDPVDAANEAMSYHND"
     gene            2025..>2173
                     /locus_tag="ACHOQW_004870"
     CDS             2025..>2173
                     /locus_tag="ACHOQW_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9575765.1"
                     /translation="MTELTKEKLIEEAKLKIAIAKCYPNSGMARVEGELFKIALASLE
                     AEPIA"
BASE COUNT          651 a          422 c          619 g          481 t
ORIGIN      
        1 cggcgacaac tggcggcacc aaaacgcccg ttgggaccaa cgccaattga gttgctgaaa
       61 caggagtatg aacgccggaa agcggctggt tttgtctgat ttgagaagta atttttatcc
      121 ggaggaaatt ttaatgggaa ccgtattgca tgcactgaaa gcgatgggta aagccaattc
      181 tgttgaactg gcggcgcggc ttgatatcag ccgtgaagaa gttctcaacg aactgtggga
      241 actcaaaaaa aatggcgttg ttgataaaac gggtcacacc tggtttctgg ctggtgaagg
      301 tgaatccggg gtaaccgaag aacagccagc acagtctgaa gtaccggatg tgctgaccgg
      361 ggaggtcgaa caaaaagtta ctgcggacat gatgattgag tttatctgtc aggatggggc
      421 taaaacgtgt gaggaactgg cggataagtt cggtgttagc attcgcaagg ttgcttccac
      481 gttggcggtc gtaacagcaa cggggagcct ggcacgcgta aatcagaacg gtaaatttcg
      541 ttactgcata ccgggcgctg atttaccggc agagccggaa gctgcatcgg taacggaaac
      601 cgatggtaaa gcctttcctc agccaacagg tgttgcgtta ccagtccggg aagcggaaac
      661 acaggaagaa ataaaaactg aaagtgtggc ggtcacagtg cagtcacagc cgttgttcac
      721 cagaaaacat ccggatggtc tgattttacc atcgctgcat gtggctaacc gcgagctgcg
      781 ccgggcaaaa ggtcaggttc agaagtggga gcgtgtctgc gccgcgctgc gggagctgaa
      841 caagcaccgg gatattgttc ggcagattgt cgattcatcc ggtcgtattg tgtcggaaaa
      901 gtgattgccg gaggcgctta tggcgaaacc ttttacacac gaacagcgtg aagaactgaa
      961 ggcccgaatt atcgggctgg tacgcaaaaa tgaacgcatg acgatatcac aactggagag
     1021 agcgacggga gcaggctggc attcagtcag acgttgcctt gtggatgtac tggcttgcgg
     1081 cgatttatac atgcccggta aatacggtgt ttttacatca gaacaggtgt atcgcgtatg
     1141 gcgtaaggca gcggagaaaa caaccgacca gacattgatt cgaaagttac cagacggaga
     1201 aatacgccgc tacgacagac aacagaacat aatctgtggc gagtgccgga agagtgaagt
     1261 tatgctgcgt gtactggcgt tctatcaggg caattttcag gaggcggtat tgtgagtgaa
     1321 ttagctatca ggcttcaatt gtcgctggca ttcacatcaa aggagaagag catggaaata
     1381 aaaccagaag atgagttaag caatatcgtt ttatttccgg taaaagagga tgacccacgt
     1441 aatcaggtta attttcttta tgagccatcg gaaagagcat attgtcatca cgcctctgtt
     1501 cgggttgacg aaaaagagcg tcaggtccgc tgtaaaatct gcggtgcagt tgtggagcca
     1561 tttgactgga tgctctctgt ggcgaaaaga gaaaccagac tggcagatga tgtaaggatc
     1621 ttgcgccagg aggaacggga aaggcggaga aatatagaaa agctaattca gattgagcgt
     1681 aacgtgaaag cgcggatacg cagggtgaca aaatccagaa ctgaataatt aaatttagct
     1741 ctgttaaaaa tttaatcctt aaccggaggt atatcaatgt caaacgcaca gaaagttatt
     1801 aacgctgaaa aatataacga gtgggtgaaa aagttctcag agcagatttt taaaattact
     1861 ggcgacgaga atgcggcaaa aaatgaatta gagccgtgga cacctgaagg tgtcgaccca
     1921 aattattgct ggtgggatgt tgatccagtt gatgctgcaa atgaagctat gagttatcac
     1981 aacgattaat gtcaggaggc cgcccgaaag ggcggtaatg aaaagtgact gaattaacca
     2041 aagagaaatt aatcgaagaa gccaaattaa aaatagcgat tgcgaaatgc taccccaatt
     2101 cagggatggc acgggtagag ggcgagttat tcaaaattgc actggcatcg ctggaagcag
     2161 aaccgatagc gtg
//
LOCUS       DAZKFZ010000372         2126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000372 DAZKFZ010000000
VERSION     DAZKFZ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1366
                     /gene="sctC"
                     /locus_tag="ACHOQW_004871"
     CDS             <1..1366
                     /gene="sctC"
                     /locus_tag="ACHOQW_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFP9575766.1"
                     /translation="RKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLV
                     VNAAKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNN
                     NIKQSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQII
                     AYPDTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGT
                     IKIGSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDN
                     NRTFYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDD
                     LPQVGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNA
                     NNIVRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNRE
                     VVGG"
     gene            1371..>2126
                     /gene="sctW"
                     /locus_tag="ACHOQW_004872"
     CDS             1371..>2126
                     /gene="sctW"
                     /locus_tag="ACHOQW_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA];
                     GO_process: GO:0050709 - negative regulation of protein
                     secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HFP9575767.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLL"
BASE COUNT          724 a          355 c          472 g          575 t
ORIGIN      
        1 gagaaaggta tcgacgaatg agtttaataa tttcttaaaa aaatcgggtc tgtataactc
       61 tcgttatgaa attaaagggg acggcaatgg tactttctat gtatcggggc caccggttta
      121 tgttgacctg gtcgttaatg ctgcgaaatt aatggagcaa aacagcgatg gcattgagat
      181 cggtcgaaat aaagtaggaa taattcatct ggtcaacaca ttcgttaatg accggactta
      241 tgagttacgt ggcgaaaaga tagttattcc tggcatggca aaagtactat cgacgttgtt
      301 aaataataac attaagcaaa gcacaggggt gaacgtactt tctgaaattt caagtcgtca
      361 acaactaaaa aacgtatcac gtatgcctcc ttttcctggg gctgaagaag atgatgacct
      421 acaagtagag aaaataatat cgacagcagg agcgccagag actgacgata tacaaattat
      481 tgcatatcct gataccaaca gtttacttgt taaaggaaca gtatctcagg ttgattttat
      541 cgagaagtta gtagctacgc ttgatattcc aaagcgacac attgaattat ctttatggat
      601 aatagatatt gataagactg atctagaaca gctaggtgct gactggtcgg gcacgataaa
      661 aattggatcc agcttaagtg catcatttaa taattcaggt tcaattagta cccttgatgg
      721 gacacagttt attgcaacaa tacaggcact tgcacaaaaa agaagagccg ccgttgttgc
      781 tcgtcccgtc gttttaacac aagagaatat tcctgctata tttgataata accgcacttt
      841 ctatactaaa ttagtcggcg agcgtactgc ggaattggat gaagtaacct atggtacgat
      901 gattagcgtg ctaccacgat ttgctgcgcg aaatcaaatt gaattactct tgaatattga
      961 agatggaaat gaaatcaatt ccgacaagac caatgttgat gatctgcctc aggttggtag
     1021 aacacttata agtactattg cacgcgtgcc ccaagggaaa agtcttttga ttgggggata
     1081 tacacgcgat acgaatacct atgaaagtcg caagatccca atattaggca gcattccatt
     1141 tattggtaaa ttatttggtt atgaaggaac aaatgcgaat aacatcgttc gtgtctttct
     1201 tattgagcca agagagatcg atgaacgcat gatgaataat gcgaatgagg ctgcggttga
     1261 tgccagagcg attacacagc aaatggcaaa aaataaagaa atcaacgatg aattactgca
     1321 gaaatggata aaaacttacc tgaatcgtga agtcgtcgga ggatagtgaa atggcgattc
     1381 atgtagagca tgtcggtgta ttagaaagag ccagggaagt ttctcgctta gaggacatta
     1441 tcacagaaga taatgaagac attgaagctg aaatgcctaa aatgagggat gatcccgcag
     1501 gtaaagaagc tcggttctta caagctactg acgagatgtc agcagcgtta acgcagttta
     1561 tgaagaaaaa aatttacgaa gaacaactgg ccaattttct ggacggagaa gaatatgtcc
     1621 tggaagacca gccaatagaa aaaaccgaca aagttatgga ggcgcttaag gctgcaacta
     1681 cccatgacta tgaagtgtat agctttgcta aaaaattatt cccagatgaa agcgatctgg
     1741 tagttgtgtt acgtgcaatt ttacgtaaaa aacaaatatc agaaaatgtc cggctaaacg
     1801 ctgaagcatt acttagaaaa gttaatcaag agactactaa aaaattcatt aatagcggaa
     1861 ttaactcggc attaaaagca aagttatttg gccaggcgct gtcattaaat ccaaaattac
     1921 taagggcgag ttatcggcaa tttttaatgg cagaagacga tgctgtagat acttatgtcg
     1981 aatggattgg tagttatggc tatcaaaata gaatgttggt gacaaaattt atcaaagaga
     2041 cgcttttctc agatataaat gcacttgatg cgagttgttc atcattggaa tttggtatgt
     2101 ttctcaataa gttgtcacaa ctattg
//
LOCUS       DAZKFZ010000373         2123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000373 DAZKFZ010000000
VERSION     DAZKFZ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            208..1350
                     /locus_tag="ACHOQW_004873"
     CDS             208..1350
                     /locus_tag="ACHOQW_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9575768.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1824..1924
                     /gene="ykgR"
                     /locus_tag="ACHOQW_004874"
                     /pseudo
     CDS             1824..1924
                     /gene="ykgR"
                     /locus_tag="ACHOQW_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
BASE COUNT          681 a          450 c          417 g          575 t
ORIGIN      
        1 cctgacattt atcctttatt gactacacaa caataataaa attattcaaa aagaaatttc
       61 attaaaaaac aaaaaagcag caactcaatt attagaaacc ctttaaaggg attataaata
      121 aagccagaac tcttggttcc aaagctggca acaactaacc agttaaaaca tacgcatcaa
      181 aatcagataa acatttgagg tattgttatg acaaataaac acccatcatt gttttctcct
      241 tttatgctga cagaaaaaat aaagctacgt aatcgtatcg ttatggctcc tatgacgacc
      301 tggtcagcga atcctgatgg aaccatctct gagcaagaac tcgaatttta taaacgtcgc
      361 tcacaaaatg ttggtttagt aataacagga tgtacctatg tcacgccaag cggcattggg
      421 tttactcatg aatttgccgc gtatgacgac cgtttcatta acagccttga aaagttagca
      481 gcggcagcca aaagtggcgg tgctccggct attttgcaga ttttccacgc cggtaataaa
      541 gccatcccgg agcttgtacc aaataacgat gttattagtg ccagcgcatc aagcgtaaaa
      601 tctggtgact ttatgaagag agtggtacaa agcagggaaa tgactgaaaa cgaaattcag
      661 gaaaccatta gagcattcgg agatgtcaca aaaagggcga ttaaagcagg ttttgatggc
      721 attgaacttc atggtgcgca cggattcctt ctacagaatt ttttctcccc tctatttaac
      781 cagcgtaatg accgatgggg aggagatctc gaaggacgta tgcgcttccc gctggccgta
      841 ttacaggaag taaaaaatgt agtctatgaa tatgcgacaa agccctttgc catcggttac
      901 agaatatctc ctgaagaatc tgcaacaggg gggctgcgga tagaggatac ttataaactc
      961 cttgatcggc tcatttcatc cggtatttcg tatatacata cctcgctggt cagtattaac
     1021 gatagctacc ctgttgagtc gccaaatggc ccccgaacta ttgaattgat tttaaatcat
     1081 attgcaggca gagtgccggt aatcgctgca ggtaaaatac gcacaccgtc tcaggctcaa
     1141 gaagcaattt ccactggcct gccacttgtt gcgatcggta aagggctggt gataaatcca
     1201 gaatgggtga ctttagctga atcaggtaga ggccatgaaa ttcaaacaac ccttaatcca
     1261 caacttgttc ctgaattaac cattccggat aaactatggg atcaaattca ggcatcgaaa
     1321 ggaactggct ggtttccgtt aatggattaa atatctatta ctctcccgag agtaaaacta
     1381 tcaaatcaat gagtaaccga tacagcctca caaattcttt cagataatta acccgtcact
     1441 gcggcacgtt ctctcataag gcgtgcctgc agtgtaatct agtatgtatc cggcatagtt
     1501 gacggtttaa tagagctcaa ttcctcgccc aggttaccaa caacgatatc ttgtataaca
     1561 ccccaggaga tacgacatta tgatgagatg cgatatgctc ctcagcgcca atcaacgcca
     1621 ggactgggcc agtcttgaga tcagactcat tcccggaact gacttcaggc gacatatatc
     1681 aatgacgttg gggcaataaa agatgcctga tgtaatacat ctattcaaat ttgtgataca
     1741 tgtcaaaaaa ttggttgacc aaactcgtta tttatataag ggcacttacg aagtgcgctc
     1801 ttttttaaag cgaggaagta ccaatgaaag agaataaagt acaacaaatc agtcataaac
     1861 tgattaatat cgttgttttg tcgcaattgt agaatacgcc tatttatttc tccatttcta
     1921 ttaataacgg caataaactg ttcacttcag tgatatttaa aatatgcatc ctctcccttt
     1981 ttgtaagtaa ttattatatc cgtgggagag gaatacacgc tgtcaggtaa tcaatcatac
     2041 tgcgataaat catcggccag taaagtggag ataacctcca ttctcgaaaa atccatactc
     2101 tcagcgaaac tatcatcaat cac
//
LOCUS       DAZKFZ010000374         2100 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000374 DAZKFZ010000000
VERSION     DAZKFZ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            72..722
                     /gene="nleG7"
                     /locus_tag="ACHOQW_004875"
     CDS             72..722
                     /gene="nleG7"
                     /locus_tag="ACHOQW_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFP9575769.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDTDQQKQHCK"
     gene            complement(947..1822)
                     /locus_tag="ACHOQW_004876"
     CDS             complement(947..1822)
                     /locus_tag="ACHOQW_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFP9575770.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(1963..>2100)
                     /locus_tag="ACHOQW_004877"
     CDS             complement(1963..>2100)
                     /locus_tag="ACHOQW_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9575771.1"
                     /translation="IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGM
                     K"
BASE COUNT          633 a          436 c          380 g          651 t
ORIGIN      
        1 aagttaaata tgaatgataa tatacctaca gcgcgaaatc acaaacaatc gacttgtatt
       61 acagaaaaaa catgcctatc tttttaaact tcacagcagg cagtatctta cctgagaatg
      121 agctagcatc tttacgttat attgtgcagc aaaatcaaaa tgatactgta atcataaaag
      181 aacgttataa aatggatatc cgttatatcg aatcagtcaa tggttttaca gtaaatcctg
      241 tatgcagtaa tcatttctcc atatttatgg cgagacaaaa cactattgct cgcaacctgg
      301 aacagcagat caacaacgga cgaagttttg cacaaatatc tcaggatttt atgcttcaat
      361 tatcttcaaa tataggatgg aaaaaagggg ccgaaaacgc ccttaaaaat aaaatccatt
      421 ctcattcatt tgttgtaaat cctgatgaat tctcttgcga cacacaattt cttaagtgcc
      481 caataacatt atgcgttcca gaaaaagggg tttttgtcaa gaacgcactg aactccaaca
      541 tatgcactct ttatgataag tctgcgttca tgaatctcac aagagaacat ctaccccacc
      601 ctctcagcag ggaaaagata gtaaaagaaa tgattattga aaggaatatg tgttattttg
      661 acaccataag tcagcatttc ataattatgg atacagacca acagaaacag cattgtaaat
      721 aaaatgtaat aattacatac tattagtgat tctcatgcat cgtaagcggc tcgccagaac
      781 cgtattgata tttactgagc gctcagatca actttccatg gcaacagatc gcgtacccgg
      841 tttgccggcc agtcctggat atgctcccgc cgacaatatt aatggccagg aaatgtttaa
      901 aacaatgatt ctgtttccta accatcaagt gtatatgctg gctcaattac tcaatatcta
      961 tgggttgagt tctacccaaa gtggctctgt agtcgcttct tcttacagta tgttcaggtg
     1021 ttgcaggcac agttgtggag cgtagatgcg ttgttggttt accatgctct ggtacataaa
     1081 aaacgccagt gccatggtaa cctgatatag aaacgttaat tccgcgacgt tcaaactcgg
     1141 catagacgtg ttctgctaaa gacctctttt gaccaaataa tgccctggcc aaccagccgt
     1201 tattcatatt tgcggatttt tctatttcat caggagagaa gtctttgttt tttattatgt
     1261 tggctgagtt acaggatgtt aatctgatat catctatttc atgaagatta tgctgaacaa
     1321 tgcggtcgac aatatctgac ggtgataaaa attcatctcc gcacttaaga agaggtagac
     1381 cagcagaacc atgcccagac agataaattt tgttgtattt tcctagtgtt aaatcatctg
     1441 gcaggatggt tttcatctct gtcgctgtaa tacttatagc tgcagcagca acaacatcgc
     1501 tattacttga ttgtagatgg cttttatttg ctccaggata tgtaaactcc attttccttt
     1561 tatcaaaatc ttgttttgta gcatcattca ggaataataa atcatacggt tttttccccg
     1621 ttgtgactct tgaaacattt tggccaagaa tattaagagt atagtcatta acggatttca
     1681 ttcctaaaaa aacaagtaat ccttctttct cttgcgcttt ttcgattgtt ctccctgcta
     1741 aagttattgg gatggattga gattgttttg tcggaactga tacgctggcg ctaataggta
     1801 gtagggttct tatgctaaac atacaacctc tttcttaatt ttcgaacctg tttaggattc
     1861 tgtgtaaatt caaaataaac ctgtctgaac attcctaaca aacatccacc ggacatgaca
     1921 acaaaaaccg gagccggact ccggtttttg tgaagctgtc ggctatttca ttccgccaat
     1981 attttcccac ctcccgtcag cacgcagaat ttgcagcggt cttaccacgc actgtatctg
     2041 ctttttatcc gcatccagta tcaccacctg cgtgattacc ctgtcctgct ccgggataat
//
LOCUS       DAZKFZ010000375         2089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000375 DAZKFZ010000000
VERSION     DAZKFZ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..144
                     /locus_tag="ACHOQW_004878"
     CDS             <1..144
                     /locus_tag="ACHOQW_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9575772.1"
                     /translation="LQLLDKVSPSQWVAIGVLGSLLFGLLTYLTNLYFKIREDRRKVA
                     RGE"
     gene            complement(400..672)
                     /locus_tag="ACHOQW_004879"
     CDS             complement(400..672)
                     /locus_tag="ACHOQW_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575773.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            832..1365
                     /gene="rrrQ"
                     /locus_tag="ACHOQW_004880"
     CDS             832..1365
                     /gene="rrrQ"
                     /locus_tag="ACHOQW_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9575774.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1586..1699
                     /locus_tag="ACHOQW_004881"
     CDS             1586..1699
                     /locus_tag="ACHOQW_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575775.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1701..2027
                     /locus_tag="ACHOQW_004882"
                     /pseudo
     CDS             1701..2027
                     /locus_tag="ACHOQW_004882"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2011..>2089
                     /locus_tag="ACHOQW_004883"
     CDS             2011..>2089
                     /locus_tag="ACHOQW_004883"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9575776.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIIS"
BASE COUNT          538 a          429 c          573 g          549 t
ORIGIN      
        1 ctgcagctgc tggacaaagt ctctccgtcc cagtgggtgg caataggtgt gctgggaagt
       61 ctgctgtttg gcctgctgac gtatctgact aacctgtatt tcaaaatcag agaggaccgt
      121 cgtaaggtgg cgcggggaga gtagtcgatg aataaacaat acgaactggt tgtaaaatga
      181 atatttctaa ctgaaaaaac gttccatgag gtaagaaaag gtcacaggca atcaataaca
      241 ggacgtgatg aaagaccctt gcatttgtgc gctttctctt tagatagcag cagatactga
      301 aaatctgagt tgtcggggag tcagggatac agctgtgcaa gagttggtca ttgtgattcc
      361 attgaaatcc tgtatgccat gaagggcagg attttatggc tacctgagct ttggtgatag
      421 taagttgaaa attcgcattt tttgctgaca tgcgtaacga gaatcccata agcagggagg
      481 acttaattct tcattaaccc atgcgttgat attatgtttc agccgttgaa gcatcagcgg
      541 tgttaatgtt gtggtaataa tatccagcgt tttatgtgag atcttaccgt aagggtctgc
      601 aagaatgctg cttgttgctt cgttattatc tgccatcaga agaagtaact ctgatttaac
      661 gttttctgtc attagttgta aaaatcttct gcgcaaactt tctttactgt tcatttatat
      721 ggcttcattt gttgtaatct gctgcgtctc aagggatatg tttatgagag cgaccatgag
      781 tgttggatta tatacctaac atatcaaggg attagaaatc gataaatccc catgaacgaa
      841 aaaataaaat acggcctgtc ggctgccgtt ctggcgctga ttggtgcagg tgcttctgcg
      901 cctgaaatcc tcgaccagtt tctggatgaa aaggaaggta accacaccac ggcataccgt
      961 gatggtgcgg ggatctggac catctgccgt ggtgccattc tggtggatgg taagcctgtt
     1021 attcctggca tgaagctgtc aaaggaaaaa tgcgaccggg ttaatgccat cgaacgtgac
     1081 aaggcgctgg catgggtgga gaaaaacatc cgggtgccgc tgaccgaacc ccagaaagcg
     1141 gggatcgcgt cattctgtcc gtacaacatt ggccccggta agtgcttccc gtcgacgttt
     1201 tataaacgaa ttaatgcagg cgatcgaaaa ggtgcctgtg aggcgattcg ctggtggatt
     1261 aaggacggtg gcagagactg ccgtatccgt tcaaataatt gctacggtca ggtctcacgg
     1321 cgtgaccagg agagcgcgct ggcgtgctgg gacatcgaca gatagcagaa tattttcctg
     1381 aaaaatgacg ttggccaacg cgggtggata acacgaaatc ctgaaaactg gtaaaaccta
     1441 agtgaataaa agtaaaaacc ccgtttgttg gcagcaagcg gggttttgtg ttttctgacc
     1501 ttgagtaagg caagggagaa attatgggta gggaggtact ttccctgtga ggaagtataa
     1561 aagattcttt ctgaggttgt ccattatgaa aggcattgaa gtggagacgc cagccagtct
     1621 ggatttaaca agagcggcag cttttgccat tcgtattgtg gccattgctg ttctggtctg
     1681 ggcaatccgt tggtggtgat atgaaccgtg ttctgtgtgt ggttatcatt gtcctgctgg
     1741 tggcctgtgg tgcgcttagt ctggggctga atcattaccg tgatcacgcc atcatctaca
     1801 aagagcagcg cgataaaaaa gccagtgagc tggagctggc gaacgcgaca attactgata
     1861 tgcagatacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg gaattagccg
     1921 atgcgagagc tgaaaatgaa actctgcgtg ctgatgttgc cgctggtcgt aagcgcctgc
     1981 ggatcaacgc cacctgctcc ggtaccgtgc gtgaagccac cggcacctcc ggcgtggata
     2041 atgcaaccgg cccccgactg gcagacaccg ctgaacggga ttatttcat
//
LOCUS       DAZKFZ010000376         2051 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000376 DAZKFZ010000000
VERSION     DAZKFZ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            40..690
                     /gene="zinT"
                     /locus_tag="ACHOQW_004884"
     CDS             40..690
                     /gene="zinT"
                     /locus_tag="ACHOQW_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP9575777.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            1033..1563
                     /gene="yodB"
                     /locus_tag="ACHOQW_004885"
     CDS             1033..1563
                     /gene="yodB"
                     /locus_tag="ACHOQW_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9575778.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          643 a          434 c          388 g          586 t
ORIGIN      
        1 cattacacat catatacaat gactctggag gaaactgttt tggcgattcg tcttcacaaa
       61 ctggctgttg ctttaggtgt ctttattgtt agcgctcctg ccttttcgca tggtcatcac
      121 tcacacggca aacccttaac agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat
      181 gccaatgtac aaaaccgaac gctcagtgac tgggatggag tctggcaatc cgtttatcct
      241 ttactgcaaa gtggcaaact tgcccccgtc tttcagaaga aagcggatgc agataaaact
      301 aaaacatttg ctgaaattaa agattattat cacaaaggct atgcaacaga tatcgagatg
      361 attggcattg aggacggcat tgttgaattc catagaaata atgaaacaac atcctgtaaa
      421 tatgattacg atggatacaa aatactcacc tataaatcag gcaagaaagg cgttcgttac
      481 ttattcgaat gtaaagatcc tgaaagcaaa gcccctaaat atatacaatt tagcgatcat
      541 ataattgcac cacgaaaatc atctcatttt cacattttta tgggtaatga ttcacagcaa
      601 tctttattaa atgaaatgga aaactggcca acgtattatc catatcagtt gagtagcgaa
      661 gaagtggtcg aggaaatgat gtctcattga gtattctcat gataacgcct cgatgccgct
      721 ttagtatgtc atcataactg ccactggtcc atccacaaac gccactgaac gcaagctagc
      781 tacagacacg ctcatcacta tgacgtgtct gtatattaat aagctaaccc gcattgagtt
      841 aaccaataac ggattccata cacaatacgg ccaacagaga aagatacatg tgctcacgcc
      901 attgcttata ttggctggtt acaatgtgca ctatcaattt tttaaataac aaattcttaa
      961 aaccacatct gtataaggaa tttttaaggt tcgtgggata gcttgactgt gaaaattaca
     1021 ggagctacaa aaatgaaccg attctcaaaa actcaaattt atttacattg gataacgctg
     1081 cttttcgttg caataaccta tgccgcgatg gaactccgtg gctggtttcc taaaggtagt
     1141 agtacttatc tgctgatgcg agaaacacat tacaatgcgg gtatattcgt ttgggtgtta
     1201 atgttttcac gcctgattat aaaacaccgt tatagtgatc cttctattgt gccaccgcca
     1261 ccagcctggc aaatgaaagc ggcttcgcta atgcacatca tgctttatat aaccttcctt
     1321 gcgcttcctc tgctggggat tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt
     1381 ggtttcaatg tgtctccctt tgttacccca aacagcgaaa ttaaagcact gataaaaaat
     1441 attcacgaaa cctgggcaaa tataggctac tttttaatcg tagctcacgc tggcgcagca
     1501 ctctttcatc actacattca gaaagataat actctgttac gaatgatgcc tcgccgcaaa
     1561 taagtcgatc ctctgacaaa ggaaatgtga accatggaca tcaacgcatc ataaatgttt
     1621 acgcaagtgc ataccgcact ctttccacta tcaacaagga ggagcagaaa atgtgtaaat
     1681 cgtacaatgg taagacttat caaaaataca ggttaatttt ggtatttaaa ccttttttac
     1741 ttgttagtat gatacaaagg ctttcaaaaa agctgggaaa ttcaacaact cttggaaatt
     1801 atggactttg tcccgcggct ttaatctgac ggccgcgttc cttttttgac tcaattcttg
     1861 accccttctc tacgcaactt cagttcccac caccaacttt gcggcagttt cataggatga
     1921 atgtctaaaa gaataatggt gagcgataag aaaacgactg aataactgca cattttcgct
     1981 cgaaagcttc ctgtcagatc catagcgaat caagtgctga atgtcacagt atcgaacaga
     2041 aaacagtgac g
//
LOCUS       DAZKFZ010000377         2045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000377 DAZKFZ010000000
VERSION     DAZKFZ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(74..766)
                     /locus_tag="ACHOQW_004886"
     CDS             complement(74..766)
                     /locus_tag="ACHOQW_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9575779.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            877..1099
                     /locus_tag="ACHOQW_004887"
                     /pseudo
     CDS             877..1099
                     /locus_tag="ACHOQW_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
     gene            1130..1669
                     /locus_tag="ACHOQW_004888"
     CDS             1130..1669
                     /locus_tag="ACHOQW_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP9575780.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            1666..>2045
                     /locus_tag="ACHOQW_004889"
     CDS             1666..>2045
                     /locus_tag="ACHOQW_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9575781.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRG"
BASE COUNT          547 a          471 c          482 g          545 t
ORIGIN      
        1 taaattatga ggatgtcagc atgtctcacc aaaacatcat ctggttatct tgatgaaatc
       61 tgtcattaat aatctaccca tgttttctat atgtctgtgg catgctccca ataactttcc
      121 caaatataaa tacccgattc atttcgtctt tttcgatcgg gtcccacggt gagtagctct
      181 tgttatcaga gataaccagc agcttatcct tcatcatttg caggcgcttt acatgggctg
      241 tgtcgtcgta cagaaacgca tagataccat caccgtcgaa agatttaaca gtgatatcaa
      301 cgaacagaag atcacctggt tcgatcgttc ctgacatgct gtcaccgcgt acgttaatga
      361 tgcggatatt ttctgccttc ctgccatcga acatgtgacg agcatcgtca aacgagtact
      421 caaccgagcg taggacttct acaaactcac ggttgatgac acccggccca gcactgactt
      481 ctatatcaag aacgtcaatc ttgaagtatt tggaatggtt gacagcaggc ttccctgatt
      541 gttgaccgtc atttctcatc gggcctatgc ctgatgagag ccactctgtt cgaacaccca
      601 atgcattagc tatttcaaca atttttgttg agccgcgtgc gttgccgctt gtcagtctcc
      661 agattgtggg ttgagctacg ccagacgcct ttgcaagagc gccttgagac attccagatt
      721 gttccatcgc taggtttaag cgatcagcaa gagtttcttt tttcataagt tttaatttat
      781 acgcttgcgt attgatggtc aaaacacgtt ttgctattga ttggattaat acgcattgct
      841 attatccatt cattgtaata ccaataggaa ttgataatga caaatcaaac cattcaactc
      901 gcaatcagta ttacaggtag tcaaaaacga ctggcagatc tatgcggtgt agcccactgt
      961 ttggcgttgg ctacacggtg gcggaattga tgcccgctat gtaatgaaaa ttgtctcagc
     1021 cactggtgga aagattaaac cagcagatat tcgtcccgac cttgcaccat tgtttaacgc
     1081 gagtaattct gccgcctaaa ctgcggcgtt aactgataag gcaatgatta tgcaaccact
     1141 tacataccaa cagactagcg gatttagccc gactgcggtg ataaatcgtt ctcaaataaa
     1201 acaggtgcca ggccacgaaa aaatccgtga tgccgtccgc gcctggtcgg ctgtagataa
     1261 tcaggatgtc gttgccacac tcattgtgaa tgagtatcgg gagcagggcg gcggcaccat
     1321 cgatttccct gatgatgtca gccgtacacg ccagaagctg ttccgcttcc tcgataacaa
     1381 attcgattct gaaaaatacc gaaataacgt gcgtgaactg accccggcaa ttctggcggt
     1441 actaccgctg gaatatcgcg gttacctggt tgagcaggat agcttcatgg ctaggttggc
     1501 tgaaatggaa aaggaactca gtgaggcaaa acaggctgtc attctcaacg caccacgcca
     1561 ccagaaactg aaggaaatta gtgaaggtat tgtgtcgatg tttcgtgtgg acccagagct
     1621 ggctggtcca ttgatggcga tggttactac catgctgggg gcgatatgac aggttcagaa
     1681 atggcgaaag ccggtctgct ggaacagaac cgactttcag gtgcaaatcg taacacactc
     1741 attgcgggag gaattatggc aaacactgct gagatattca attttccagt gccggatgcg
     1801 gcacaaaagg agccgcgcgt ggcagatctc gatgatggtt atacgcgcat tgcaaatgag
     1861 ttgctggaag ctgtaatgct ggccggatta acacagcacc agcttctggt cttcctggct
     1921 gtcatgcgca aaacatatgg ctttaataaa aaactggact gggtgagcaa cgagcaactt
     1981 tccgagttga ccgggatatt gccgcacaag tgttctgctg caaaaagtgt tctggtaaag
     2041 cgtgg
//
LOCUS       DAZKFZ010000378         2034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000378 DAZKFZ010000000
VERSION     DAZKFZ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(61..987)
                     /locus_tag="ACHOQW_004890"
     CDS             complement(61..987)
                     /locus_tag="ACHOQW_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9575782.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            1144..>2034
                     /locus_tag="ACHOQW_004891"
     CDS             1144..>2034
                     /locus_tag="ACHOQW_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9575783.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDK"
BASE COUNT          596 a          463 c          436 g          539 t
ORIGIN      
        1 atgattacgt tattcctctt accgatattc gcccagagca accatcaatt acacatatca
       61 tcaattttcg agttttactc ttttctccac cataaaatca ataaaagcac gaactttaga
      121 gggcattctc acccgtgatg aaacataaat ataaaaccca ggaaatgaaa cagcccaatc
      181 ctcaagaatc ggctgtaact tgcccgcatt gatatgttca gcgattgcaa aatccagatg
      241 ttgaatgata cccgttccat tcagcgccat tcgtatcatg ctttcgtcat cattagaaat
      301 atacttacct gaaacctcta tttcgaactc ttcattaccc cccacaacct tagaaaactc
      361 ccatgcagag agtgcaccgc tacttttatg ccgcaaccga aggcaattat ggttactcag
      421 ttcctgaggc gtttcaggaa taccgtgttc ttttaaataa tctggcgatg ccacagtaac
      481 cagtttaata agcggagaaa caggaacggc agtcatatgc tcatccagtc cttgctcaag
      541 acgtatgccc acatcacagc cgtccgcaat aatgttagat aaaccgtcat ccataatgag
      601 ttctatcttt gttttggggt aacgagtaag aaactcgcca atatgaggct caacaagcat
      661 ccgtgctgca acacgggagg aatttattct caacaggcct gtaggttcaa gttgggtatc
      721 accaacatct ttcaaagcat catcaatttg ccccaaagcc gacaccagca cttcatacag
      781 acgctgtcct tcctcagtaa gcgagatatt gcgggtcgta cggtatatca gcttggtgtt
      841 aagacgttcc tcaagagatt ttaaattctg ggacaatgcg gccagcgtaa tacccatttc
      901 agaagcggct ctggaaaaac tgccggcacg aacaatcagc gcaaaccagg caagagaagg
      961 caaaattgaa ggctctattt tcatcattgt aaaccttaac ttaacactgt gatagatatt
     1021 agaccattga tgataaccaa tacaagtcat actattacgc cgttctcgat atgaacacaa
     1081 atgccagttg cggataatag aaatgatatt taaatctgtt taggcaaatc cccggagctt
     1141 gaaatgaaaa ctgtttcgat aaaacacact tactgggata tagctgctga tatctatttc
     1201 cctgacgatt ttaatagtga aaaaaagtat cctgccatta tcagcgccca tccaattgga
     1261 agctgcaaag aacagacatc gggtagtgtg tatggtgccg ccctggcaaa agctggcttt
     1321 atcgtcattg cttttgatgc tagttttcag ggaagcagcg gtggggaacc gcgttatctg
     1381 gaagatccaa caatgcgagt aaaagacttc agcattgtcg ttgattacct taccagctta
     1441 ccttacgttg atgccgggcg tattggtgta ctgggtattt gtggtggagg tggatatgcc
     1501 attaatgccg caatgacaga acgccgaatt aaagctattg gtactgtcac gggtgcaaac
     1561 tacgggcgtc ttatgcgtga gggatttaca gcgtttaatc ctatcggagc acttgaagca
     1621 atggcgcaac aaagaacaga cgaagctaac ggcgcaaaac ttcgcgtaga tgatttactc
     1681 ccctcttctc cggatgcagc tcttgaagcg ggactaacag aaatagacct ttttgaagcc
     1741 acagaatatt atcgttcgcc acgaggctat gcacctaatg gggtaaatcg ttcgctattc
     1801 tcacatcaga ctgttgccgt gggttgggat gcatttcatc ttgcagaagt gctgttaact
     1861 caaccaataa tggttgtggt gggcgataga gtgggcgcat ttggtgccta ccgtgacggc
     1921 tgcgaaatta ttggccgcgc tgcttcaaaa cataaagagc tggtcgtggt tgaaggttat
     1981 tcacattacg acctgtatga taagccagaa cctgtgaaac aggcactcga taaa
//
LOCUS       DAZKFZ010000379         1910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000379 DAZKFZ010000000
VERSION     DAZKFZ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(322..1605)
                     /gene="gltA"
                     /locus_tag="ACHOQW_004892"
     CDS             complement(322..1605)
                     /gene="gltA"
                     /locus_tag="ACHOQW_004892"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP9575784.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1811..>1910)
                     /locus_tag="ACHOQW_004893"
     CDS             complement(1811..>1910)
                     /locus_tag="ACHOQW_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575785.1"
                     /translation="SYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT          515 a          421 c          460 g          514 t
ORIGIN      
        1 tcatgtaata atattaatgt tgctacatta tgtttgagag tttctcaaag ctctcgaatc
       61 ataaataaag tgaattaatc ctattacgag tttatttaat tgtgggtgct acccaatgta
      121 ttctacatcg attggcggga taacggtggt ggtgaatgaa aaattaggat tttatttagt
      181 tatcggatat gtattcttgt aacgtgtaat tatgtatttg tgtccgatta cgtttttttg
      241 tcgatagaca taactaacga acaaaaaaaa gcccgccaga tattcaggcg ggctaaacga
      301 tatatggttc aaatcagata attaatgttt aacatctgat ttaaagtctc gtttttcata
      361 gccggtgtac aactggcgcg gacgggcgat cttcatgccg tcactgtgca tttcgctcca
      421 gtgagcgatc cagccaacgg tacgggccat agcgaagatg acggtgaaca tggaagacgg
      481 aatacccatc gctttcagga tgataccaga gtagaaatcg acgttcgggt acagtttctt
      541 ctcgataaag tacgggtcgt tcagcgcgat gttttccagc tccatagcca cttccagcag
      601 gtcatctttg gtgcccagct ctttcagaac ttcatggcag gtttcacgca ttacggtggc
      661 gcgcgggtcg taatttttgt acacgcggtg accgaagccc atcaggcgga aagaatcatt
      721 tttatctttc gcacgacgaa caaattccgg aatgtgttta acggagctaa tttcttccag
      781 cattttcagc gccgcttcgt tagcaccacc gtgtgcaggc ccccacagtg aagcaatacc
      841 tgctgcgata caggcaaacg ggttcgcacc cgaagagcca gcggtacgca cggtggaggt
      901 agaggcgttc tgttcatggt cagcgtgcag gatcagaata cggtccatag cacgttccag
      961 aatcggatta acttcatacg gttcgcacgg cgtggagaac atcatattca ggaagttacc
     1021 ggcataggag agatcgttgc gcggataaac aaatggctga ccaatggaat acttgtaaca
     1081 catcgctgcc atggtcggca ttttcgacag caggcggaac gcggcaattt cacgatgacg
     1141 aggattgtta acatccagcg agtcgtgata gaacgccgcc agcgcgccgg taataccaca
     1201 catgactgcc attggatgtg agtcgcgacg gaaagcgtgg aacagacggg taatctgctc
     1261 gtggatcatg gtatgacggg tcaccgtagt tttaaattcg tcatactgtt cctgagtcgg
     1321 tttttcacca ttcagcagga tgtaacaaac ttccaggtag ttagaatcgg tcgccagctg
     1381 atcgatcggg aaaccgcggt gcagcaaaat accttcatca ccatcaataa aagtaatttt
     1441 agattcgcag gatgcggttg aagtgaagcc agggtcaaag gtgaacacac cttttgaacc
     1501 gagagtacgg atatcaataa catcttgacc cagcgtgcct ttcagcacat ccagttcaac
     1561 agctgtgtcc ccgttgaggg tgagttttgc ttttgtatca gccatttaag gtctccttag
     1621 cgccttattg cgtaagactg ccggaactta aatttgcctt cgcacatcaa cctggcttta
     1681 cccgtttgtt atttggctcg ccgctctgtg aaagagggga aaacctgggt acagagctct
     1741 gggcgcttgc aggtaaagga tccattgatg acgaataaat ggcgaatcaa gtacttagca
     1801 atccgaatta ttaaacttgt ctaccactaa taactgtccc gaatgaattg gtcaatactt
     1861 ccacactgtt acataagtta atcttaggtg aaataccgac ttcataactt
//
LOCUS       DAZKFZ010000380         1893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000380 DAZKFZ010000000
VERSION     DAZKFZ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(674..>1893)
                     /gene="nleL"
                     /locus_tag="ACHOQW_004894"
     CDS             complement(674..>1893)
                     /gene="nleL"
                     /locus_tag="ACHOQW_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFP9575786.1"
                     /translation="DKVRAAEELIKKISLDELAAFRPYVKMSLADSFSIHPYLNNANI
                     QQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHPGAMVSYNSSFIQIVM
                     NGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKPDWDDDSDLAYNWVLL
                     SSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIF
                     SIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYKMIADDQQRKLACVWNP
                     FLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEI
                     LRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFTCTAVLTDMLTEHAKESF
                     PEIFSLYYPVAWR"
BASE COUNT          682 a          397 c          249 g          565 t
ORIGIN      
        1 taattcattg tgtcccatga ttttatagtt gagaatgaac catgagtacg acttgttggc
       61 agtatatagt ttgttatttt cattaaaatt ccacttcctc ttataacgca ctattcaatg
      121 aataaacacc agttgagagt cttttcaaaa caatcattaa ttacgccata ttactatagt
      181 aacaaaaaca tattaatctg aatatcaatt cataaaatat gattctccac gatttgccca
      241 gaaataacga acataatttt cgttgtaatt aaaatcatat ttcatctata gtgaccattc
      301 acagcaaaac aaaatcaatt atcacattca aaaaaatact gacaacaaag atactcttca
      361 gacaaagttg tcatatgtaa attttccctt aaaacgttgc tcaagaaaaa tataaaaatc
      421 atatgcccta atcaacatcc tcaagcacta cgatatatgc agtatcctat tcatatatta
      481 ccaccatatc agacaaatcg aatcacaacg agttctatcg tactctttca tcttcgacac
      541 tgtaaatttt aagaacattc ggctcatgcc actaaaccaa gacataataa caacggagca
      601 ataaatgttc ccactgaaca tgtgcaaacc taaaaagaca gtgttacaat aatattcatc
      661 catggtctct aaatcaacgc cacgcaacag gataatacaa tgaaaatatt tcaggaaaag
      721 attcttttgc atgctccgtt agcatatcag tcagcacagc ggtacaagta aaaacattat
      781 ttcttccttg tagcctgtct aaaatatcat tgtaataata aactgagcca aatacctgag
      841 gatctgtttt ataagcttgt tcaatcaatc catttgcata tctccgtaga atctcaggag
      901 aatcatactc tgtaccaaac atatcactcg atgagtattt acagaaaacc ccccctaaac
      961 aaaataatat ttcagcctgt tttcttagtg gcatatcttt caatacatgg ctccccataa
     1021 tcatatctac atgctgagcg ttcagttccc aaccatcaag aaagggattc cagacacagg
     1081 caagtttcct ttgctgatca tcagcaatca ttttataatc tgatttattg gaattaagtg
     1141 cctcaataaa tctctctttg agttcattat cagaaatgag tatatccaga ataccagaaa
     1201 cgaaattctg ggaaaaagct ttatgataag gaatactgaa aattggaaat gattcagaaa
     1261 aaagattgct caatgaatac ccaaatgtat tttgaacttc tccatcctta tataaaaaaa
     1321 aggacatcca attagttgat gtattaggag ataacatatc aaccatatga ctcaaagaac
     1381 acatcattgc cataccatca tcctgtgatg ataacaaaac ccagttataa gccaaatcgg
     1441 aatcatcatc ccagtcaggc ttcccagagc catcgcacaa tccaaaatca ctctgctgtg
     1501 tgacaggata aactttttca ttttttaaat atacttcata gagttcccta aatcgttcct
     1561 gcattccatc acgccgtgaa ccattcatta caatttgtat aaaggaacta ttataagata
     1621 ccatcgcacc tggatgtcgc tcaaaataca tccctgcctg caataaacta ccattctcca
     1681 tatacatcat tattgaatta ttaaaccaag acattatagt atcaaaaaag tcatcacata
     1741 taggctcgag ccattgctgt atatttgcgt tgttcaaata aggatgaata ctaaatgaat
     1801 cagccaaaga catcttaaca tagggccgga acgccgctaa ttcgtcaaga gatatttttt
     1861 tgataagttc ttcagcggcc cttactttat cat
//
LOCUS       DAZKFZ010000381         1873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000381 DAZKFZ010000000
VERSION     DAZKFZ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..104)
                     /locus_tag="ACHOQW_004895"
     CDS             complement(<1..104)
                     /locus_tag="ACHOQW_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575787.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIV"
     gene            complement(88..>1873)
                     /locus_tag="ACHOQW_004896"
     CDS             complement(88..>1873)
                     /locus_tag="ACHOQW_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9575788.1"
                     /translation="CGERAFVILPWLHRETARSFGGAGSTAGYEQVTAYTLTGQLQSR
                     HLNLPQLDCDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATD
                     PAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDE
                     RTHHYHYDNQHRLVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSR
                     KPEVTWYGWDGDRLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKL
                     QQEGSEDGHGVVFPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPE
                     YTPARTLHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQ
                     QHDEESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIEN
                     MTSGGLIYAVSGVPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMT
                     KTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYN
                     IGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          362 a          580 c          458 g          473 t
ORIGIN      
        1 aatacgatgt cagaatttga attgttatca aacccaaggt aaacaacaaa taaacaaagg
       61 gtaattaaaa aaatgacaag tttgcctcta ataatttgaa gcatctgaac tcccttgctt
      121 tgcttttatt ggattatctg cttttatacc atctaacccg aagagttgcc caatgttata
      181 tttttcaata catgcatcgc tacatttggc agaaaaatcg ccacaagcaa taccgacagt
      241 gttattttta agatccatga ttctttcttt agctggctga ccttgtctat ccccagccgc
      301 ctcatgattt ttacctatca cgtcagctat tgttgatcca aatgtcttag tcattcgaca
      361 catcaagtaa caatatctca ttgcatcggc ggccccatta tgagctccgc caacaatagc
      421 atccatatca taaccgaact ggtaagcact gttagtaatg ctgtttgcaa caatcaatcc
      481 aggtacacca gatacggcat aaattagtcc acctgatgtc atattctcaa tcgcatctaa
      541 ccccattggg tctatcactt gtatgggatt caacggatac tgatacatat tccatccacc
      601 tctcaaccca atcgggtccg gggtgatata ccgcccctgc aacggatcgt agtaccggtg
      661 acggttatag tacagccccg actcctcatc atgctgctgg cccggcagac ggtacggctg
      721 gtgcaggtga tgcgggttct cctcattaag ctggttgccc cattcatcat attccgcgct
      781 ccacgccgta ttgccgtctt cgctgataag cgccagcggc agtccccggt ggtcacagtg
      841 gtacagatgc agcgtgcgcg ccggcgtgta ttccggctcc acctgttttt ccagttgctc
      901 caccgtcagt ccgcactgcg caagccacgc ccggctttca ctgctcacgc ggtctgcccg
      961 gatttcttcc tccagcctgt ccagcatcct caccagttct gccgggaaca ccacaccgtg
     1021 accgtcctca ctcccttcct gctggagttt ctccgccagg ctgcggcgct gcgctttctc
     1081 ccgctcgccg ttctccgttt cgatgcggat gagcggtgtg aagctccccg gccggtatac
     1141 cgtctggata cgtgtggtgc cggtctgtac cgtcgtcagc ctgtcgccgt cccacccgta
     1201 ccaggtcacc tccggtttac gcgacagcga catccacccc gtcaggtcac gctcccgccg
     1261 ccacacccgt ttccccgtcc ggcggcccag cgggtcatag aggtagcggc tctcgaccag
     1321 cggctcgccg tattgtatcc gcgtgtagaa caccaggcgg tgctggttgt cgtaatggta
     1381 gtggtgggtc cgctcgtcgt gcatccggat aaccccttcc ggaatgcggt ccgtcttctc
     1441 cgtcagcctg ccgtattcat cgtagcggta gacatagtgc gcatcttccg cgatgcggtt
     1501 atccggccac gccgtgagcg tgctgtccgg atgcagttcc gggtccggca gccggtttcc
     1561 tgccgggtcc gttgcatacg ggatatcgat atccaggttc gctgccgtgg tgtggacgcc
     1621 cgtcagcctt cccgtgtcgc tgtaacggta ctcccggctc tcctgcgggc cgctgatgcg
     1681 gattagctgt ccgttgtcgt tccagtcgta gtcacagtca agctgcggga ggttcaggtg
     1741 cctgctctgt agctgccctg tgagggtgta cgccgtgacc tgttcgtacc ccgctgtact
     1801 gcccgccccg ccgaagctgc gggccgtctc acggtgcagc cagggcaaga ttacgaaagc
     1861 ccgctccccg caa
//
LOCUS       DAZKFZ010000382         1853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000382 DAZKFZ010000000
VERSION     DAZKFZ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..426
                     /locus_tag="ACHOQW_004897"
     CDS             <1..426
                     /locus_tag="ACHOQW_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9575789.1"
                     /translation="ASPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGG
                     QRQRIAIARALVTNPRILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTV
                     KNANRIIVMDNGFISEDGTHKELISKKDSLYAYLYQLQA"
     gene            430..>1853
                     /gene="ehxD"
                     /locus_tag="ACHOQW_004898"
     CDS             430..>1853
                     /gene="ehxD"
                     /locus_tag="ACHOQW_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP9575790.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINE"
BASE COUNT          635 a          285 c          401 g          532 t
ORIGIN      
        1 gcttctccgg ctgtatctat ggaacaggct attgaggcag ccagacttgc aggtgcccat
       61 gattttatta gagaactaaa agaagggtac aatactattg ttggagaaca gggcgttggt
      121 ctttcaggag ggcagcgcca acggatcgca atagcccggg ctcttgttac aaatcctcga
      181 attcttattt ttgatgaagc aaccagtgct cttgattatg agtcggaaaa tataataatg
      241 aaaaatatgt caagaatatg taagaacaga accgtaatta ttattgcaca caggttgtca
      301 actgtaaaaa atgcaaacag aataattgtt atggataatg gctttatttc tgaagatggc
      361 acacataaag agcttatctc caaaaaagac agtttatatg catatttata tcagttgcag
      421 gcataacgta tgcgctttta catgaaaggt ttatgggatt tggtttgccg ttataagacg
      481 gttttttctg atgtctggaa aattcgtcac acacttgatg ctcctgtgag agaaaaagat
      541 gagtatgcat ttcttcctgc tcaccttgaa ctcattgaaa caccagtatc cagacgttct
      601 cattttgttg tatggagtat tttattattt gtaattatat ctcttctctt atctgttctt
      661 gggaaagttg aagtggtttc agtagcaaat ggtaagttta ctcatagtgg aagaagcaaa
      721 gaaataaaac cgatagagaa tgcgattgta gaaaaaataa tggtaaaaga tggctctttt
      781 gtaaaaaaaa atgatccatt agttgaattg acggtgcctg gtgttgaatc tgatatttta
      841 aaatcagaag catctttgtt gtatgaaaaa acagaacaat atcgctacgc aattctttct
      901 gaatcaatac aacggaatga gcttcctgaa ataaggataa cagattttcc tggcggagaa
      961 gataatgcgg gaggtgaaca ttttcagagg gttagctcat taataaaaga gcaattcatg
     1021 acatggcaga acagaaaaaa tcaaaagcag ctcacattaa ataaaaaaat agttgaacgg
     1081 gatgcagcgc ttgctcgtgt tagcctgtat gagcatcagg tatcacagga aggaagaaaa
     1141 ctcaatgatt ttaagtattt gttgaataaa aaagctgttt ctcaacattc agttatggag
     1201 caggaaaata gctatattca ggcaaaaaat gaacatgcag tctggcttgc acaggtttct
     1261 caacttgaaa aagaaataga acttgtgcgg gaagaactgg cactggagac gaatatcttc
     1321 agaagtgaaa ttatcgagaa gcacagaaaa tcaacagata acattgtgtt gctggagcat
     1381 gagcttgaaa aaaacaggca gagaaaagca tcatctttta ttaaagctcc tgtgagtggt
     1441 actgttcagg agttaaatat acatacagaa ggtggtgttg tcacaacagc agaaacgctg
     1501 atgattattg tccctgataa tgatattctc gaagtaacag catctgtact taacaaggat
     1561 atcggtttta tacaacctgg acaggaggtc gttattaaag tagatgcata tccatataca
     1621 cgtcatggtt accttacagg gaaagtaaaa aatataactg cagattctgt ttctgttccg
     1681 gatacagggc ttgtatttaa cgtgattata tctgttgatc ggaatgatat acagggagaa
     1741 agaaaaaaaa tccctgttac agctggaatg actgttatgg cagaaataaa aaccggggtg
     1801 cgtagtgtta tcagctatct tcttagtcca ttaaaggaaa ctattaatga aag
//
LOCUS       DAZKFZ010000383         1836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000383 DAZKFZ010000000
VERSION     DAZKFZ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1195..1797
                     /gene="fimB"
                     /locus_tag="ACHOQW_004899"
     CDS             1195..1797
                     /gene="fimB"
                     /locus_tag="ACHOQW_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP9575791.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          547 a          328 c          389 g          572 t
ORIGIN      
        1 tcaattcatt ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc
       61 tttcaggctg aagtgcgttt tcatcacccg atgagaaaga actgaagtga tgtttgccat
      121 agattgcaag atgccctgtc tcattgtcta tcgtggtgca cctttatacc tgttatacca
      181 gatcgaaaat cacgcaatcc atacaacaaa accagatttg caattcgtgt cacaaaatat
      241 gtcgatcttt ttctaagaag aagatgccat gtgaagccag acgaacactt gcggtggtct
      301 tcaaaaacta aagatcttcg tttaactatt tgttttataa ataatttatt tagagtctaa
      361 acaagggaag ctttgcaagc taactcagtg agcttggtgt aaatcagtgt ttacccgcca
      421 tcaggctgag cataattctc atcatgaaat atgtttcctg gtttgtggct tgtaactggt
      481 cacttctgaa gtcgatctgg agaggcttat tgatgttggt gttttcagga tgatgtttca
      541 cttagtttgt ttgccgtatc gcccggcgaa tggctgtgat tgaggaaggt ttaagtcgta
      601 gtgaccaaag ctatatttac caacgaatgt agatgaaaaa atcatctcct gcgttccccc
      661 atatctccag gataaaaagg aatgtaacaa tctcatggcg taagctgacg aatcagccag
      721 gaataatcgc tagggaccta agaattagct tgataatagc cactaagaaa ttactgtgtt
      781 ccatgaaata gccattttgt ggcaaatgga gttgactaat aatgtcatat gtgagacggc
      841 tagttgaacg aatattaaat tttgctgaat tttttatgtt gattttactt gttacagaac
      901 atatcacatg atatatagat aagattagtt gcattaatga tgagggttat tattagattc
      961 gtatccgatt gataaatata taaaggtaca tagcatgcaa gagcatagcg tttgtatggc
     1021 aacgttatta taattaactg ttgctactcc atttaagttc actcagaaga actggtccac
     1081 ttacgttagt tattaagcaa acgttcgctt ttataaacat aatcaggata aaaatgttgg
     1141 attattgcta acctagcaca gctagtgcgc gtctgtaatt ataagggaaa aacgatgaag
     1201 aataaggctg ataacaaaaa aaggaacttc ctgacccata gtgaaatcga atcactcctt
     1261 aaagcagcaa ataccgggcc tcatgcagca cgtaattatt gtctgacttt gctttgtttt
     1321 attcatggtt tccgggcgag tgaaatttgt cgattgagga tttcggatat tgatcttaag
     1381 gcaaagtgta tatatatcca tcgattaaaa aaaggctttt caacaacgca cccgctattg
     1441 aacaaagaag ttcaggcttt aaaaaactgg ttgagtatcc gtacttctta tccgcatgct
     1501 gagagcgagt gggtattttt atcgcgtaag gggaatccgc tttctcggca acaattttac
     1561 catattatct cgacttccgg tggtaatgcc gggttgtcac tggagattca tccgcacatg
     1621 ttacgccatt cgtgtggttt tgctttggcg aatatgggaa tagatacgcg acttatccag
     1681 gattatcttg ggcatcgcaa tattcgtcat actgtctggt atactgccag caatgcaggg
     1741 cgtttttacg gcatctggga tagagccaga ggacgacagc gtcacgctgt tttatagaga
     1801 tagttgagat accagggatg gtgttaaaaa ctacat
//
LOCUS       DAZKFZ010000384         1835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000384 DAZKFZ010000000
VERSION     DAZKFZ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..555
                     /locus_tag="ACHOQW_004900"
     CDS             <1..555
                     /locus_tag="ACHOQW_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9575792.1"
                     /translation="ALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGT
                     VIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLG
                     ASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISQDISTRDEGGGGTVVVVGRQG"
     gene            898..>1835
                     /locus_tag="ACHOQW_004901"
     CDS             898..>1835
                     /locus_tag="ACHOQW_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9575793.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPERPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVP"
BASE COUNT          439 a          455 c          573 g          368 t
ORIGIN      
        1 gcgctgtcgt tacaggtgcc gggctttcgc cgtcagatga acgaaggctg gtaccagata
       61 cgtattgccg gttatgacac ggcaccggag gcggtgtacg cccgtcttca cgaacagctg
      121 ggtgagggaa cggtcatcca tattgtgccg cgactggccg gggccggaaa gggtggactg
      181 cagattgtgc tgggggcggc agccatcgtg ggctctttct tcactgccgg ggcatcaatg
      241 gcgttatggg gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttca
      301 cttggagcca gcatgattct gggcggtgtg gcccagatgc tggccccgaa ggcaaaaaca
      361 ccggattacc gcgcaacgga taacggcaga cagaacacgt acttttcctc gctggataac
      421 atgattgccc aggggaaccc gatgccggtg ccttacgggg aaatgctggt tggctcccgc
      481 cgtatatccc aggacatcag cacccgtgat gaaggcgggg gcggaacggt cgtggttgtc
      541 gggcgacagg gataaaacat aaaaaaatcc cgcagtgatc gcggagctgc ggggacagac
      601 aaatgaagat caatgttaag gagttgtttt tgttactcgg gcaaaaaaac actaacgcag
      661 cgaaattata agcgccacag tcagtgtgtg aaaatgtgaa gatattcaga atttttatgc
      721 cattaccggt tttaaccaac aggattatcg gtgggcatga aagaaaaccc cggtatctgc
      781 tgataccggg gtttctcttt agcatggcag aaatgtgttt catgcttttc gggcgaagga
      841 tatccgacct ctgtacggaa tggcaagtgg cggttaattt attcagggga aggctgtatg
      901 ggaaaaggtg gcggtaaggc acacacgcct cgtgaggcga aggataatct caaatccacg
      961 cagatgatga gtgtgattga tgcgattggt gagggaccga tagaaggtcc ggtgaaggga
     1021 ctgcagagta ttctggtgaa caaaacccca ctgacggaca cggacggcaa tcccgtgata
     1081 cacggtgtga ccgcggtctg gcgtgccggg gagcaggagc agacaccacc ggaaggcttt
     1141 gagtcctccg gagctgaaac cggactgggc gtggaagtga cgaaggcaaa accggtgacg
     1201 cgcaccatta cgtccgcgaa cattgaccgc ctgcgggtca ccttcggggt gcagtcactg
     1261 ttggagacca cctcaaaggg cgaccgtaat cactcttctg tccgactgct gattcagttg
     1321 cagcgtaacg gtaactgggt gacggaaaag gatgtcacca ttaacggcaa gaccacctcg
     1381 cagtttctgg cgtcggtgat tctggataat ctgcctgagc ggccctttaa catccggatg
     1441 gtccgggaga cagcggacag cacctcggac cagctgcaga ataagacgct ctggtcgtca
     1501 tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca cggcgattgt ggggctgcag
     1561 gtggatgcgg agcagtttgg cggtcagcag atgacggtga actaccatat ccgcggtcgc
     1621 atcatccagg taccgtcaaa ctatgacccg gaaaaacgca cgtacagcgg catctgggac
     1681 ggcagcctga aaccggcata cagcaacaac cctgcctggt gcctgtggga catgctgacc
     1741 cacccgcgct acggaatggg aaaacgcctg ggggcggcgg atgtggacaa gtgggcgctg
     1801 tatgccattg cgcagtactg cgaccagacg gtccc
//
LOCUS       DAZKFZ010000385         1800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000385 DAZKFZ010000000
VERSION     DAZKFZ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.389"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            157..456
                     /gene="espZ"
                     /locus_tag="ACHOQW_004902"
                     /gene_synonym="sepZ"
     CDS             157..456
                     /gene="espZ"
                     /locus_tag="ACHOQW_004902"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFP9575794.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
     gene            540..917
                     /gene="sctI"
                     /locus_tag="ACHOQW_004903"
     CDS             540..917
                     /gene="sctI"
                     /locus_tag="ACHOQW_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP9575795.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            920..1492
                     /gene="escJ"
                     /locus_tag="ACHOQW_004904"
     CDS             920..1492
                     /gene="escJ"
                     /locus_tag="ACHOQW_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFP9575796.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1498..>1800
                     /gene="sepD"
                     /locus_tag="ACHOQW_004905"
     CDS             1498..>1800
                     /gene="sepD"
                     /locus_tag="ACHOQW_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFP9575797.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKD"
BASE COUNT          605 a          338 c          358 g          499 t
ORIGIN      
        1 gaatattatt gcttgaaaaa gcgtattgga taatatatac agtatatgta ctttatgtac
       61 attgcaacta tttaaataat ataatagttg cctatgggat aatttggtta tttataacca
      121 ggtaaatgtc taaattagac aaaaggagaa agatcaatgg aagcagcaaa tttaagtcct
      181 tctggtgcag taatgccgct ggcgacctca ctcagtggaa ataactcagt ggatgagaag
      241 acaggagtga ttaaaccaga aaatggaaca aatcgcaccg ttagagttat agccggatta
      301 gcacttacca ctacggctct ggcagctcta ggtacaggta ttgcagcggc atgctcggag
      361 acgagcagca cagaatactt agccctgggt attacttctg gcgtactagg tactcttact
      421 gcggttggcg gtgcattagc gatgaaatat gcctaaccgg cccatcgcat aaataaaaga
      481 tagaggtaat ggatgtatta tgcccttgcc tctttcataa aaagaggcta acagtaaata
      541 tgaacaacat taatcaatcg gaaaacatta atattcaatt aaataaagcg ccccaaacaa
      601 atttcgttga tgagcatacc tcgttagcct cagccccctc tgcggcaggt gcggctcagt
      661 ttcttgatca attactccct aaaacagcgg gagtgtcttc tccagaacaa gtgttgattg
      721 aagaaattaa aaagagacat cttgcaacaa tgaacagcga tctcagtttc gatgctctat
      781 ctgcaggtgg gctctcgcca gaagacgtgc tcaccttaca aaagaatgtg ctcaacgcaa
      841 acgtcaacgt tgatgtcgta tctaagttag caagcctcct ttcaacatcg gttacaaaat
      901 tagtttcgat gcaataaaaa tgaaaaaaca cattaaaaac ctttttttat tggctgctat
      961 ttgtttgaca gtggcatgta aagagcaatt gtatacagga ttaacagaaa aagaagctaa
     1021 tcagatgcaa gcactgttgc tatccaatga tgtcaatgtt tccaaagaaa tggacaaaag
     1081 tggaaatatg acactcagtg tggaaaaaga ggattttgtt cgcgcaataa cgattttaaa
     1141 taataacggc ttcccgaaaa agaaatttgc agatatcgag gttattttcc cacctagcca
     1201 gttagtggca tcgccatcac aagaaaatgc aaaaatcaac tatctcaaag aacaagacat
     1261 cgaaaggttg ctaagtaaaa tacctggtgt tatcgattgc agcgtttcat taaatgttaa
     1321 caataacgaa agtcaaccct cctcagcagc cgttttagtc atcagctcgc cagaggttaa
     1381 cttagcacca tcggtcattc agataaaaaa cctagttaag aatagtgttg acgatctcaa
     1441 attagaaaat attagtgttg ttatcaagtc atcctcagga caggacgggt aatatgtatg
     1501 aacaataata atggcatagc aaagaatgat tgtgactggc taacggccct tgattttgta
     1561 aaggatgtca acggttcacc aacgcatctc actttttata tctaccaaaa aaatgctttc
     1621 ttgcacgatt ttggaaacta ctgggttcta tatattgagc ttagtggtga ttttcgtcag
     1681 gttccaacag atacatttat cagactctgc aatatcctgg ccgttagtaa cgaatataaa
     1741 caaatgggta ttttcctgtc taataagaag tggtatcttt gccaaatttt tcataaagat
//
LOCUS       DAZKFZ010000386         1792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000386 DAZKFZ010000000
VERSION     DAZKFZ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            140..643
                     /locus_tag="ACHOQW_004906"
     CDS             140..643
                     /locus_tag="ACHOQW_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP9575798.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHOQW_004907"
     CDS             complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHOQW_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP9575799.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHOQW_004908"
     CDS             complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHOQW_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP9575800.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1437..1760
                     /gene="yecR"
                     /locus_tag="ACHOQW_004909"
     CDS             1437..1760
                     /gene="yecR"
                     /locus_tag="ACHOQW_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP9575801.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          465 a          428 c          393 g          506 t
ORIGIN      
        1 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
       61 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
      121 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
      181 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
      241 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
      301 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
      361 tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
      421 cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac tgaatgatga
      481 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gcctgttgct
      541 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc cagtgcagac
      601 cgaccaacat gttctgaatg tcgtgtcgca tcagctgcat tgatcataca tcgacgctaa
      661 tgcattgcac cgatgaagtt tttgagaaac cgctgcctca tcttttttga agcagcggtt
      721 ttttaatggg attcgccttg tggggtaaac ttgagctcaa tcagcgcgat ggctttttgg
      781 attgcccgca tggtgaccgg gtctgcggca gcgggatgat gagtaaagtc gatattcttc
      841 agctgactgg acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga
      901 atttgtttga taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc
      961 agcgtctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc
     1021 acatcttcaa cgatagtcac atcccccctc atcaggacac aaaaaaacct gccggagcag
     1081 gttttttgtt atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta
     1141 ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata
     1201 caggtgttat tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt
     1261 gatacaaatc acataaatac ccctttaatg ttataaaaat gataatcaaa gaacagcccc
     1321 ctatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt ttccggccat
     1381 tgtcgcagcg ctgtaacgcg taaaatagtg ctttctctta ctcttctggc tggaccatga
     1441 gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt actcgccagg
     1501 cccatgtgag cgaagttgac gcggcaacag gtattgtacg gttggtttat gaccaggctt
     1561 ttttgcagca tgcccatacc gatcgttatg tcagtcgcgg gatctccgat cgcgcatgcc
     1621 aacaggaagg ctatacccac gcgatcccct ttggtcagcc agtaggcaac tgtagccttt
     1681 ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc catcattccg
     1741 ccttccctgt ctttctctaa atattttaat cagcgagggg atcttcgctg at
//
LOCUS       DAZKFZ010000387         1774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000387 DAZKFZ010000000
VERSION     DAZKFZ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1347
                     /gene="espN"
                     /locus_tag="ACHOQW_004910"
     CDS             <1..1347
                     /gene="espN"
                     /locus_tag="ACHOQW_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFP9575802.1"
                     /translation="QFIWGFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVL
                     HFLGTIWNMAMGAVPGYNALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRS
                     DLHSRSLQIKYEALKHIEKNINDNIIYHPSNNANFYSVIESIDGNDFIYNEKQSKILE
                     MKQDRGGNRYSAVDLNSSKYGYYEKVGGGFYRYIESFNPISSETPNKIVYKGESVDLT
                     KEPNSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREGDI
                     GYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIILCR
                     GTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADRFS
                     REHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          559 a          276 c          383 g          556 t
ORIGIN      
        1 cagtttattt gggggtttga taaggttatt caagaaggct taagtggatt gattgagtta
       61 agtatcgata ttaatgatag tacaaatcat cgtgataagt cttctctttc tcctgatggg
      121 agagctgtgt tacatttttt aggtacaatt tggaatatgg cgatgggagc tgtacctggt
      181 tataatgcat tgtctggtgt aagtagcatt ttacatagtg ctatagtaaa agaatctagc
      241 aatatctgtg attatattca gggggctgta cgtattggaa tggactttgt tccaggcact
      301 cgctctgact tacatagccg ttcgctgcag ataaaatatg aggctttgaa gcatatagaa
      361 aaaaacatta atgataatat tatttatcat ccgagtaata atgctaattt ctattctgta
      421 attgagtcaa ttgatggtaa tgactttata tataacgaaa aacaatctaa aatattagaa
      481 atgaaacagg atcgtggggg gaatagatat agtgcagtag atcttaactc ttctaagtat
      541 gggtattatg agaaagttgg cggtggtttt tatagatata tagaatcctt taaccccata
      601 tcttcagaga caccaaataa aatagtctac aagggggaat cagtagattt aactaaggag
      661 ccaaattcgg aattatattc aggtaggtat tctataaata acaaacaggt taatgtttat
      721 ttctttcgtg acgctgatgg tacattttat aaatcagaag gtcttcatgg tgggggagtt
      781 attagataca tagataaacc gtattctcag ttaagagaag gagatattgg gtatgatgag
      841 gatttgttgg atatatacga tgattctccg gtgcttgaag acacgttgcc tgctttatct
      901 tctgaaatag taccaactcc agaacatagt attaaacaaa tttattcgaa aattaaggag
      961 gggcacatag aactgtccga ttcagacatc atattgtgtc gcggcacaac cggtattcaa
     1021 gctgaaaata tcgttgaata taaaactgct ggagggcttc ctgattcaaa tccaaatgta
     1081 aaagcaccag atgaatatat ggcacaacag caggtacgta ttggaagaat attgcctgaa
     1141 tacacatcgg atcttagcgt tgctgatcgg tttagtcgtg agcattatct aatagttgtt
     1201 aaagtaaagg caaaatatat cacacgagga agtgttacag agagtggttg ggttatagat
     1261 aagaccgcac ctgttgaacc acttgcgata attgatagaa cttttggtat gaaggaaaat
     1321 atctcaatgg taaatgcatc gaaatagttt ttttacaatc tatgtcctgc ctcctctggt
     1381 aaaaacgatg ctacatcttg gagatgttgc acggcagtac ggtgttgacc gatatgtagt
     1441 gaagtacaca tcggtcaacg aattaccatt gtcagcaata tcatcctgat aaactcagta
     1501 ctcgtgagcc gcttgatgac gggcggaata gcccaaaagg taaacgatcc gcatggcagt
     1561 ccgtcggaaa gctgctgctt catacgaccg cttaaaccgt cagttagtgt cagtatcgct
     1621 gaagatcagc ttcttttgct gatttactct gtttttacct gccctgatga aatactcttt
     1681 aatcataaca ttgattatat cgaaaatata ttttttgcta tcattaagaa tatttatatg
     1741 tggaaccgga gtattttgga cggtagtctt tcca
//
LOCUS       DAZKFZ010000388         1753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000388 DAZKFZ010000000
VERSION     DAZKFZ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(109..552)
                     /locus_tag="ACHOQW_004911"
     CDS             complement(109..552)
                     /locus_tag="ACHOQW_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9575803.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
     gene            709..1638
                     /gene="metA"
                     /locus_tag="ACHOQW_004912"
     CDS             709..1638
                     /gene="metA"
                     /locus_tag="ACHOQW_004912"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP9575804.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          431 a          476 c          392 g          454 t
ORIGIN      
        1 aagcgaaagg ccatcctgac ggatggcctt tttgcgttgg tgcaaaaaaa tgccggatgc
       61 gacgctggcg cgttttatcc agcttacgca ggcacgatag ggggcagctt attcccccac
      121 atacgccaga tccagcaacg gatacggttt ccccaaatcg tccacctcag agcgtcccgt
      181 aaccttaaaa cccaccttct tatagaaccc aaccgcctgc tcattttgct cattaacatt
      241 ggttgtcagt tccggggcca ttgagagcgc atgcttcacc agcatccggc ccacgccgca
      301 gccgcgcaca tcaggatcga taaacagcgc atccatatgc tgcccactta acaacataaa
      361 cccaaccggc tgatcccgtt cattaaccgc gacccacaac ggtgcttccg gcagaaagga
      421 acgaaccagc tcttccagct cggcccgata ctctgctgat aaaaaatcgt gggtggcatc
      481 gacagatcga caccaaatcg caacgagttc ctccccttcc tcatgccgtg agcggcgaat
      541 actaataacc attttctctc cttttagtca ttcttatatt ctaacgtagt cttttccttg
      601 aaactttctc accttcaaca tgcaggctcg acattggcaa attttctggt tatcttcagc
      661 tatctggatg tctaaacgta taagcgtatg tagtgaggta atcaggttat gccgattcgt
      721 gtgccggacg agctacccgc cgtcaatttc ttgcgtgaag aaaacgtctt tgtgatgaca
      781 acttctcgtg cgtctggtca ggaaattcgt ccacttaagg ttctgatcct taacctgatg
      841 ccgaagaaga ttgaaactga aaatcagttt ctgcgcctgc tttcaaactc acctttgcag
      901 gtcgatattc agctgttgcg catcgattcc cgtgaatcgc gtaacacgcc cgcagagcat
      961 ctgaacaact tctactgtaa ctttgaagat attcaggaac agaactttga cggtctgatt
     1021 gtaaccggtg cgccgctggg cctggtggag tttaatgatg tcgcttactg gccgcagatc
     1081 aaacaggtgc tggagtggtc gaaagatcac gtcacctcga cgctgtttgt ctgctgggcg
     1141 gtacaggcag cgctcaatat cctctacggc attcctaagc aaactcgcac cgacaaactc
     1201 tctggcgttt acgagcatca tattctccat cctcatgcgc ttctgacgcg tggctttgat
     1261 gattcattct tggcaccgca ttcgcgctat gctgactttc cggcagcgtt gattcgtgat
     1321 tacaccgatc tggaaattct ggcagagacg gaagaagggg atgcatatct gtttgccagt
     1381 aaagataagc gcattgcctt tgtgacgggc catcccgaat atgatgcgca aacgctggcg
     1441 caggaatatt tccgcgatgt ggaagccgga ctagacccgg aagtaccgta taactatttc
     1501 ccgcacaatg atccgcaaaa caaaccgcga gcgagctggc gtagtcacgg taatttgctg
     1561 tttaccaact ggctcaacta ttacgtctac cagatcacgc catacgatct acggcacatg
     1621 aatccaacgc tggattaatc ttctgtgata gtcgatcgtt aagcgattca gcaccttacc
     1681 tcaggcacct tcgggtgcct tttttatttc cgaaacacac ctcagtaggt gaataaattt
     1741 tattcatatt gtt
//
LOCUS       DAZKFZ010000389         1732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000389 DAZKFZ010000000
VERSION     DAZKFZ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..197)
                     /locus_tag="ACHOQW_004913"
     CDS             complement(<1..197)
                     /locus_tag="ACHOQW_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9575805.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHR"
     gene            239..424
                     /locus_tag="ACHOQW_004914"
     CDS             239..424
                     /locus_tag="ACHOQW_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9575806.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(554..694)
                     /locus_tag="ACHOQW_004915"
     CDS             complement(554..694)
                     /locus_tag="ACHOQW_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575807.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1051..1275)
                     /locus_tag="ACHOQW_004916"
     CDS             complement(1051..1275)
                     /locus_tag="ACHOQW_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575808.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1340..1546)
                     /locus_tag="ACHOQW_004917"
     CDS             complement(1340..1546)
                     /locus_tag="ACHOQW_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9575809.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
BASE COUNT          498 a          452 c          378 g          404 t
ORIGIN      
        1 cggtgatcga gttcactgtc acagaggtca accacacgac cacagagaca gcactgcccg
       61 gcatccctga gccagatatg acgcttgagg gaaacacgtg cactgccact gacacgacgc
      121 tgttcaccct tcagaatatt cacccgccgg gtgttcagag ttttgattct gcccggtaac
      181 gtacgaagca cagccatgta aaatcctcgc catatagctt gtcaccagag gaaagaaaat
      241 gtcatcgaaa aaccggcccc gcagaacaac aacccgcaac atccgatttc caaaccagat
      301 gattgaacag atcaatatcg cccttgaaca taaaggttcc ggtaactttt cagcgtgggt
      361 tattgaagcc tgcagaagaa ggctgtcaac agagcgttcg ggtatgaatt acataattaa
      421 gtaacatggt gttcacagaa cacacagtta ccggacacat cagttttcca ttcgtccccc
      481 ggcagtacag gcttcccgtc tgacgggata gcctgaaaaa acacagaaaa ttatttgtta
      541 taattaatat aacttactca aaaaaaagcg acgagaaaat cagcatcaac gagcaataag
      601 cgccaatacg tgataacaaa tggcagccat atttatctgc agtataagca atggacagga
      661 taaccacacc agaaaccgtc agcataaaat ccatttgaac ttccccggac aaaatcgact
      721 catctaaaga tttacagctc tttttattat caatatgtta aaagtaaaat aaacagatgt
      781 tcaataatac gaatacaaaa acgtgctgaa aatcaatgaa tccatttctg tgttatcaat
      841 taatagtgat aaacatccgg cttcttccac catcgcaccg gaccagcgac catgagggga
      901 caacgccgcg ctccgttaac gcggtaaacc ccggtgtgta tcgtttttga ttatccccgc
      961 acactcgcgc agaggattct cccggtcggg ctgcggtctc tgttaatgca ggaatacggc
     1021 gacaataccg cgcatgaata ataaggtcgc tcaacacact ggctgtaatg cagcggatac
     1081 catgcggcat ttagcggcat tcatcgtaca ctcaacggtt agctcttcat tcgtggcatt
     1141 cacctgaaag gtcctggagt gtaattgcgt acatttacca ctgaacgaac cttcaacaag
     1201 aacacgacca cgctgcaaaa tacggaacgg aattgttccc tgaaaaggct ttacggttac
     1261 caataatttc ttcatgcatt ctccgaataa caaaaatact agttaataca ctgagtgcgg
     1321 atatattcct gaagcattct caatgctgcc tggtcgctga tgattccgtc tctgataccg
     1381 agaacgtttc gtccagcaac cggagagagt tcgacggcgg catcattgcc cacgccggag
     1441 gtgccggtgg cttcacgcac ggtaccgggg caggtggcgt tgatccgcag gcgcttacga
     1501 ccagcggcaa catcagcacg cagagtttca ttttcagctc tcgcatcggc taattccctc
     1561 gagtattttg catcgagcgc agcaacatca cgctggcgca cctgcatgtc agtaatggtg
     1621 gcattcgcct gttccagctc tctggctttt ttatcgcgct gcgctttgta ggtgagcgcg
     1681 ttgtcacggt aatggtttgt tgccagccac agcgcaccac agccaaccgc ca
//
LOCUS       DAZKFZ010000390         1720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000390 DAZKFZ010000000
VERSION     DAZKFZ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1248
                     /gene="sctN"
                     /locus_tag="ACHOQW_004918"
     CDS             <1..1248
                     /gene="sctN"
                     /locus_tag="ACHOQW_004918"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFP9575810.1"
                     /translation="INGSLIEAELDDVSVGEVCEIYASRQANERIARAQVVGFRNGKT
                     LLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQIMERFVPDTPGDRGNL
                     RLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFASAGCGKTVLMHMLVN
                     NTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSDFSSVDRCNAALMATT
                     VAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYPASVFDSLPRLLERPG
                     PTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLAGQGHYPAIDVLKSVS
                     RVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNAENDFAMNARPKLTNW
                     LKQSVNEKMPMSETLKELERIVK"
     gene            1238..1696
                     /locus_tag="ACHOQW_004919"
     CDS             1238..1696
                     /locus_tag="ACHOQW_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFP9575811.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
BASE COUNT          552 a          318 c          424 g          426 t
ORIGIN      
        1 ataaacggct ctctgattga agcagaactt gacgacgttt ctgtgggtga agtatgcgaa
       61 atttatgcga gcaggcaagc aaatgaacgt atagccaggg ctcaggtcgt tggctttcgc
      121 aatgggaaga cattattaaa ccttataggc agctctgtgg ggcttacacg tactgcagtt
      181 ttgaaaccaa cgggagagca gttaaccata cagatcagtg atgcctttct tggttcggtt
      241 ttgaatgcct ctggtcaaat tatggaaagg tttgtcccgg acactccagg cgatagggga
      301 aatttacgcc tgattgatga actgccaccg tcgtatcagg aacgtcgagt tattaataca
      361 ccacttgaga cacggataag agtcattgat ggcgtactga catgtggcat tggtcaacgt
      421 gttgggattt tcgcctcggc tggctgtggc aaaacggttt taatgcatat gctcgtaaac
      481 aatactgaag ctgatgtgtt tgttattggc ttgattggcg aacgtggtag ggaagttacg
      541 gaatgcgcgg aatcgctcaa aaaatcagta aatgcagcga aatgcgtatt ggtctatgcc
      601 acctctgatt tttcgtcagt agaccggtgt aatgccgccc tgatggcgac aaccgtggca
      661 gagtattttc gagatcgagg aaagagagtt gtacttttta tcgactcaat gacgcggtat
      721 gcacgagcac tacgtgatat gaaactggca gcgggtgaac cacctgccag acgtggttat
      781 cctgcttctg tttttgacag tttgcctcgc ttactggaga ggccgggacc aacactaaaa
      841 ggaagtatta ccgagtttta taccgtgtta ctggaagggg aagatgaatc agacccactg
      901 ggtgacgaaa ttcgctcaat ccttgatggc catatctatc taagtcgcaa actcgcgggt
      961 caaggtcact atccggcaat agatgttcta aaaagtgtga gtcgagtatt tggtcaggtc
     1021 actgatgaaa aacacagaga taatgctgca cgggtaagga agaacctcac aactttggaa
     1081 gatctacagg tgtttattga cttaggggaa tatcgcgcag ggcaaaatgc tgaaaatgat
     1141 tttgctatga atgcccggcc taaactcaca aattggttga aacagtcagt taatgaaaaa
     1201 atgccaatga gtgaaacgct aaaagagctt gaacgaattg ttaagtaaag ttaatcgact
     1261 tatcagaaga acagcgcaga gcctggctgc atgtgaagca tcattacaaa agttaaatgc
     1321 agaaaaagaa aagttagccg aaaaggaacg cctgtatgat atgcaactta aaaacctgca
     1381 gtcactgtta gatatgaagg agttactcgg cgaagttgta tttcgccagg atatatttta
     1441 ctcgttacgt aaagttacag tcattcagca gcagatcgct gagataaatc ttgaaaagca
     1501 aaaaatagcc gagcggcgaa aaatacttaa caaggaaata gttcaacagc aagcacaaag
     1561 aaaacactgg tggctaaaag gagaaaaata cgaccgacta aaaaagcgca tcaaaaaaca
     1621 acttttaaat cagatgcttt atcaagacga gcttgaacag gaggaaaaat ataatggacg
     1681 aagtcaagaa aattgaacat tgtaaaattt taaacacaga
//
LOCUS       DAZKFZ010000391         1693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000391 DAZKFZ010000000
VERSION     DAZKFZ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..194)
                     /locus_tag="ACHOQW_004920"
     CDS             complement(<1..194)
                     /locus_tag="ACHOQW_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575812.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALA"
     gene            complement(244..546)
                     /locus_tag="ACHOQW_004921"
                     /pseudo
     CDS             complement(244..546)
                     /locus_tag="ACHOQW_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(573..995)
                     /locus_tag="ACHOQW_004922"
     CDS             complement(573..995)
                     /locus_tag="ACHOQW_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9575813.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQKTTKHD
                     VVRINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKTEATEPVTSKKHSKAS"
     gene            complement(1009..>1693)
                     /locus_tag="ACHOQW_004923"
     CDS             complement(1009..>1693)
                     /locus_tag="ACHOQW_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575814.1"
                     /translation="GDAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDADW
                     TATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVS
                     SIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKVTPTSGTVAKGKTTTLTV
                     SFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEV
                     TVTEAGAAG"
BASE COUNT          353 a          526 c          387 g          427 t
ORIGIN      
        1 gccagcgcct gtcggctcag actctgctca gcagcatcag cctgttttct gaaagactga
       61 ctgactttcc cggacatcaa atcagcaagc gcactggttt cacccagctc tttttttacc
      121 cgcgctgcct cttcagaaaa acgggcagaa tccagtgtca gcacggctgt cagatcggca
      181 aaattacctg ccatcgtggt cacctcctga aatgtcctct gataccatca ataactgtca
      241 caacctcctt ccctcctccc cgaaacggac tccaccggcg aggcccgccg ccttctgcat
      301 cagtacatca ttttcgtccg gcgtctccgc ctgtccttcc tctgcctccg gagcgaacag
      361 gctgaaatcc gccggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacat
      421 cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc agtaaaaacg
      481 tcgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcggctct
      541 ccccatctca cgcgccagtt tcaggacaaa gttcagctcg ccttcgaatg cttttttgat
      601 gttaccggct cagtcgcttc tgttttcccg gttggttcag gatcggcatc gtgccggtta
      661 tccagcatac ctgaaagata aagcacccgg ttcgttgcct gattcagtgc atcagcaggc
      721 catcccagca tcacttcacg gcggatctgc tgcatctctg tctccggaga ggccagagtg
      781 cctttgaggg aatgggaatg ccatagcgac atcgccacaa gcagggatgc cgtttccaga
      841 tatcgctggt taatgcgcac gacatcatgc ttcgttgtct tctgttgttc tgcgtctgaa
      901 acaaacttca tataatcaaa ccgctgcagc gcagacaatt cggaaagcgt gacggacaca
      961 ccgttatatt caaattgttc tgttttcaga aacatgtatt acctccgttt accctgcagc
     1021 gcccgcttca gtaacggtga cttcagccac tgcggcgaac tgaccatttc cgctcaccac
     1081 agggatctgc accttacctg tcgccacgcc gtttaccgta attgtcatat ctttcacact
     1141 aatggtggct ttcgacggat cggcggaaac cgctctgaac gtcttgtcgg ttgcactttc
     1201 cggctcaaaa gaaaccgtca gggtggttgt tttccctttt gccaccgtac cggatgtcgg
     1261 tgtcacctta attgcagtgg ccggcgtaat ttcgctgcgt tcttccgcca cggaaggttt
     1321 gcccacgtta gtgactttca ccgtgcgggt gatcacttct ttcgccgtca cggccttacc
     1381 gatactgctg acccagccac ggaacacatc caccgtgcca tttgggaaac ggattttata
     1441 ggcccgcaca tccccgcttt caaaccagcc tataagccct ttctgacctt cttctcccgg
     1501 tttccaggcc agcgtaaaac tggtatctcc tgcagacttc tgcccctgcc cggtcgcggt
     1561 ccagtccgcg tcttcatcat ccaggtagtt atcatcgtag ggttctgccg tcatctcgcc
     1621 cggcgtcaga tccttcacct tagccagtcg ctgccagtca tcgtctgaca acgggtttgc
     1681 ataagcatca ccc
//
LOCUS       DAZKFZ010000392         1690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000392 DAZKFZ010000000
VERSION     DAZKFZ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1058
                     /gene="escC"
                     /locus_tag="ACHOQW_004924"
     CDS             <1..1058
                     /gene="escC"
                     /locus_tag="ACHOQW_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFP9575815.1"
                     /translation="LSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVP
                     GVVSILKTMASNGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQ
                     QLISELDIEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISS
                     SVISNASNFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASL
                     ESITSGTLLRVTPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPE
                     VQNSEMTTEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSV
                     VRLFLIKATPIKSASSE"
     gene            1072..1527
                     /gene="cesD"
                     /locus_tag="ACHOQW_004925"
     CDS             1072..1527
                     /gene="cesD"
                     /locus_tag="ACHOQW_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFP9575816.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
BASE COUNT          558 a          322 c          337 g          473 t
ORIGIN      
        1 ctttatcaga aagtcttgat aaagaggcac agtctaaagc caaaaataaa gatgttgtta
       61 aggtctttaa actgaactat gcgtcagcga cagatataac atacaaatac cgtgaccaaa
      121 atgttgtcgt cccaggtgtt gtctccattc tgaagacaat ggcaagtaat ggttcacttc
      181 ccagtaccgg taaaggtgcc gtcgagcgaa gcggtaacct gtttgataac agtgtaacga
      241 tttccgctga tccacgtctt aatgcagttg ttgtaaaaga ccgcgaaata acgatggata
      301 tctatcagca attaatttct gagcttgata ttgaacaaag gcaaattgaa atttctgtct
      361 caattattga tgttgatgca aacgatcttc agcagctcgg cgtaaactgg tccggtacgt
      421 taaatgcagg acaaggtacg attgccttta atagctctac cgcacaggct aatatcagct
      481 cttctgtgat aagtaatgcc agtaatttta tgattagggt gaatgcgtta caacagaact
      541 cgaaagctaa aatactctct cagccttcaa ttataacctt aaataatatg caggcgattc
      601 ttgataaaaa cgtaacgttt tatacaaaag tctccggaga aaaggtggcg tctctggaaa
      661 gtattacaag cggaacattg ctgagagtga caccacgtat acttgatgac agctcgaata
      721 gccttaccgg aaaacgtcgg gaacgagttc gtttgttact tgatattcag gatggcaatc
      781 aatcgactaa ccaaagcaat gctcaggatg ccagcagcac cttacctgaa gtacaaaatt
      841 cagaaatgac aacagaagcg acattaagtg caggagaaag tttattactt ggcggattta
      901 tccaagataa agaaagttca agtaaagatg gtataccgct gttaagcgac attcctgtta
      961 ttggtagttt attctccagt acggtaaaac aaaaacatag tgttgttcga ctctttttaa
     1021 ttaaagcaac cccgataaaa tctgcatcta gcgaataata aatgaggctt tatgagcagg
     1081 aaatttagct ctctagagga tatttatgat ttctaccagg atggtggcac attagcgtca
     1141 ttaacaaatc tgacacaaca agatctcaat gaccttcatt cttatgccta tacagcatat
     1201 caatctggtg atgttataac cgcaagaaat ctattccatt tgctcacata tctggaacac
     1261 tggaattatg actacacctt atctctgggc ttatgtcatc agcgtttatc aaatcatgaa
     1321 gatgcacaac tgtgtttcgc acgctgtgca actttagtta tgcaagatcc cagggcatct
     1381 tattattctg gaattagcta cttactcgtc ggaaataaga aaatggccaa gaaagccttt
     1441 aaggcttgtt taatgtggtg taatgaaaaa gaaaaataca ctacatataa agaaaatatt
     1501 aaaaaattgt taggtaatac agagtaaaaa ctaccatcag ctataccaat ccccaggctg
     1561 actggggatt ttctatcgga aataataaag ctgctatagt gaagtgctcc caaagttgag
     1621 caccgaccac tggtagattt atctacaggt gtggggttat tccattaacc aggagtttat
     1681 gccctgaact
//
LOCUS       DAZKFZ010000393         1676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000393 DAZKFZ010000000
VERSION     DAZKFZ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            89..1063
                     /gene="cysB"
                     /locus_tag="ACHOQW_004926"
     CDS             89..1063
                     /gene="cysB"
                     /locus_tag="ACHOQW_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP9575817.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1382..1522
                     /gene="ymiA"
                     /locus_tag="ACHOQW_004927"
     CDS             1382..1522
                     /gene="ymiA"
                     /locus_tag="ACHOQW_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP9575818.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1525..>1676
                     /gene="yciX"
                     /locus_tag="ACHOQW_004928"
     CDS             1525..>1676
                     /gene="yciX"
                     /locus_tag="ACHOQW_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP9575819.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAED"
BASE COUNT          439 a          369 c          388 g          480 t
ORIGIN      
        1 cacctttttt attattaaat cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt
       61 ctgatggcgt ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga
      121 ggtggtcaat cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc
      181 cgggatcagt aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg
      241 aagcggcaag cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg
      301 cgaagtcctg tcgaaagttg atgccataaa atcggttgcc ggtgagcaca cctggccgga
      361 taaaggctcg ctgtatatcg ccaccacgca tacccaggca cgctacgcgt taccaaacgt
      421 catcaaaggc tttattgagc gttatccccg cgtttctttg catatgcacc agggttcgcc
      481 gacacaaatt gctgatgccg tctctaaagg taatgcagat ttcgctatcg ccacagaagc
      541 gctgcatctg tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt
      601 agtcactccg gatcacccgc tggcaggcaa aaaagccatt accattgaag aactggcgca
      661 atatccgttg gtgacatata ccttcggctt tactggacgt tcagaactgg atactgcctt
      721 taatcgcgca gggttaacgc cgcgtatcgt tttcacggca acggatgctg acgtcattaa
      781 aacttacgtc cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt
      841 cgccgatccg gaccttgtgc gtgttgatgc tcacgatatc ttcagccaca gtacaaccaa
      901 aattggtttt cgccgtagta cttttttgcg cagttatatg tatgatttca ttcagcgttt
      961 tgcaccgcat ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ccaatgaaga
     1021 aattgaggtc atgtttaaag atataaaact gccggaaaaa taatttcttg cgttattttc
     1081 ggcacctttt atgtagcgaa ggtgccggaa tatattctct tttgttactt atttgtatac
     1141 cgtgcacaat atttttatcc ctcatattta tagggtaaat tacctgtaaa gtagtgtcta
     1201 attacccgcc tcgcaaattc cagttttcaa atatctattt caggtcaaaa gattgaatta
     1261 ttcactggag acgattcgta aattcgctgg atttttgact aaagtttctt taggatttgt
     1321 ctcatcgtat gattaattac accaatcgtt caacgtcttg ctgataagtg atggcgatta
     1381 tatgaggtta gcaatgcctt ctggaaatca ggaaccccgc cgcgatcctg aattaaaacg
     1441 taaagcctgg ctggcggttt ttcttggttc tgcacttttc tgggtggttg tcgcactgct
     1501 gatttggaaa gtgtggggat aactatggtt ggtcaggagc aactggagtc gtcaccatta
     1561 tgccagcata gtgacaatga acccgaaacg aaacgggaat gttccgtcgt tattccagac
     1621 gactggcaac taacatcgca gcagcaagcc tttatagaac tgtttgctga agatga
//
LOCUS       DAZKFZ010000394         1675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000394 DAZKFZ010000000
VERSION     DAZKFZ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(454..1026)
                     /gene="hdeD"
                     /locus_tag="ACHOQW_004929"
     CDS             complement(454..1026)
                     /gene="hdeD"
                     /locus_tag="ACHOQW_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP9575820.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1281..1613
                     /gene="hdeA"
                     /locus_tag="ACHOQW_004930"
     CDS             1281..1613
                     /gene="hdeA"
                     /locus_tag="ACHOQW_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP9575821.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT          560 a          356 c          333 g          426 t
ORIGIN      
        1 gacttaagct ggcaattgga ttgccagctt tcttttaacg cattgaaatt acctgagtaa
       61 ataacgcatt catgtcttca ggtttttata tagcgtctgg agacatggat atttatgcaa
      121 tgtaatcaac gccagttgca aaaaaatgaa cccggattac ttatttaacc gcccatagca
      181 tcaacgctta cggaactctt tgttcacttt cccacagcac ttttaaaaca ttaaacctga
      241 ctacggaaaa tatcagccat gataatgttt gaaatggcta atttgccatg gagtgaaaaa
      301 aattagatga aattcagtag gttgaaataa tcactagcag gtaattattt caatgatagt
      361 gcgcaattga tctacaacac tgcgtagcgg agagagtatt aatcggatca tagtcacatc
      421 aagtgactat gatccgggtg acaaccggga taattattgc tgcttaacga acaaactggc
      481 gaagctgaac aggctggcgg cgctaaatat cagttcaatt cccaccagtg tggaaaccag
      541 cgttacagac accatcggcg ttgcaccaag gaatatccag gcaatgacga tatccagcac
      601 accaataacg agctgtagcc agctgccttt cattgaacgc tgacgatacc aactcatcag
      661 gcgaataacc cctgcaacac agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc
      721 cagctccggt gcgcggatga agaaatagcc gatcaataaa taggcgactg cgacgaggaa
      781 accggataat accggccaga aattatgact gcggttgctg aataatccaa caataagcgc
      841 aatgcccgaa cagattaata atgcacctac tactgtgctt aaaatatcgc cagaaacgaa
      901 cgggaaactg atacacagca acccgacgat aaacagcagc acggcaataa actggattgc
      961 tctgcgatgc tttttcagca tctccagatc aaacttcaaa attgttgcct tatctatata
     1021 taacatagaa ccaccctata aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa
     1081 aacgcgtcta agaatgcagt cgatttaata aaaatttcct aattgcagta tctgatgcat
     1141 ctgtaactca ttgtattgaa ataaaaatat ctgattttga tattttccat caacatgaca
     1201 tatacagaaa accaggttat aacctcagtg tcgaaattga ttcgtgacgg ctctttcact
     1261 ttatagttga ggatattacg atgaaaaaag tattaggcgt tattcttggt ggtctgcttc
     1321 ttctgccagt tgtgagcaat gcagcggatg cgcaaaaagc agctgataac aaaaaaccgg
     1381 tcaactcctg gacctgtgaa gatttcctgg ctgtggacga atccttccag ccaactgcag
     1441 ttggttttgc tgaagcgctg aacaacaaag ataaaccaga agatgcggtt ttagatgttc
     1501 agggtattgc aaccgtaacc ccagctatcg ttcaggcttg tactcaggat aaacaagcca
     1561 actttaaaga taaagttaaa ggcgaatggg acaaaattaa gaaagatatg taattccggg
     1621 aatgcgttac atcgtacttc cttgcatatt gaacaggccg gaatatcttc tttaa
//
LOCUS       DAZKFZ010000395         1638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000395 DAZKFZ010000000
VERSION     DAZKFZ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.399"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1638
                     /gene="tir"
                     /locus_tag="ACHOQW_004931"
     CDS             <1..>1638
                     /gene="tir"
                     /locus_tag="ACHOQW_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFP9575822.1"
                     /translation="PNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGSRALFTPVRN
                     SMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQIGSSVFRVE
                     TQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGGAGHAMVTVA
                     SDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQTSTSTSSLRS
                     DPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATETATRDQLTK
                     EAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAIENNAQAQKK
                     YDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQTTTTTTTTT
                     TTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSSIGTVQNPYA
                     DVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLLGNPSAGIQS
                     TYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGS"
BASE COUNT          452 a          366 c          442 g          378 t
ORIGIN      
        1 tcccaatgtg aataattcaa ttcctcctgc acctccatta ccttcacaaa ccgacggtgc
       61 aggggggcgt ggtcagctca ttaactctac ggggccgttg ggatctcgtg cgctatttac
      121 gcctgtaagg aattctatgg ctgattctgg cgacaatcgt gccagtgatg ttcctggact
      181 tcctgtaaat ccgatgcgcc tggcggcgtc tgagataaca ctgaatgatg gatttgaagt
      241 tcttcatgat catggtccgc tcgatactct taacaggcag attggctctt cggtatttcg
      301 agttgaaact caggaagatg gtaaacatat tgctgtcggt cagaggaatg gtgttgagac
      361 ctctgttgtt ttaagtgatc aagagtacgc tcgcttgcag tccattgatc ctgaaggtaa
      421 agacaaattt gtatttactg gaggccgtgg tggtgctggg catgctatgg tcaccgttgc
      481 ttcagatatc acggaagccc gccaaaggat actggagctg ttagagccca aagggaccgg
      541 ggagtccaaa ggtgctgggg agtcaaaagg cgttggggag ttgagggagt caaatagcgg
      601 tgcggaaaac accacagaaa ctcagacctc aacctcaact tccagccttc gttcagatcc
      661 taaactttgg ttggcgttgg ggactgttgc tacaggtctg atagggttgg cggcgacggg
      721 tattgtacag gcgcttgcat tgacgccgga gccggatagc ccaaccacga ccgaccctga
      781 tgcagctgca agtgcaactg aaactgcgac aagagatcag ttaacgaaag aagcgttcca
      841 gaacccagat aatcaaaaag ttaatatcga tgagctcgga aatgcgattc cgtcaggggt
      901 attgaaagat gatgttgttg cgaatataga agagcaggct aaagcagcag gcgaagaggc
      961 caaacagcaa gccattgaaa ataatgctca ggcgcaaaaa aaatatgatg aacaacaagc
     1021 taaacgccag gaggagctga aagtttcatc gggggctggc tacggtctta gtggcgcatt
     1081 gattcttggt gggggaattg gtgttgccgt caccgctgcg cttcatcgaa aaaatcagcc
     1141 ggtagaacaa acaacaacaa caactactac aactacaact acaagcgcac gtacggtaga
     1201 gaataagcct gcaaataata cacctgcaca gggcaatgta gatacccctg ggtcagaaga
     1261 taccatggag agcagacgta gctcgatggc tagcacctcg tcgactttct ttgacacttc
     1321 cagcataggg accgtgcaga atccgtatgc tgatgttaaa acatcgctgc atgattcgca
     1381 ggtgccgact tctaattcta atacgtctgt tcagaatatg gggaatacag attctgttgt
     1441 atatagcacc attcaacatc ctccccggga tactactgat aacggcgcac ggttattagg
     1501 aaatccaagt gcggggattc aaagcactta tgcgcgtctg gcgctaagtg gtggattacg
     1561 ccatgacatg ggaggattaa cgggggggag taatagcgct gtgaatactt cgaataaccc
     1621 accagcgccg ggatccca
//
LOCUS       DAZKFZ010000396         1608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000396 DAZKFZ010000000
VERSION     DAZKFZ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..845)
                     /locus_tag="ACHOQW_004932"
     CDS             complement(<1..845)
                     /locus_tag="ACHOQW_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9575823.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICG"
     gene            1114..1230
                     /locus_tag="ACHOQW_004933"
                     /pseudo
     CDS             1114..1230
                     /locus_tag="ACHOQW_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1335..1411)
                     /locus_tag="ACHOQW_004934"
     tRNA            complement(1335..1411)
                     /locus_tag="ACHOQW_004934"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1375..1377),aa:Arg,seq:tct)
     gene            complement(1425..1501)
                     /locus_tag="ACHOQW_004935"
     tRNA            complement(1425..1501)
                     /locus_tag="ACHOQW_004935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1465..1467),aa:Arg,seq:tcg)
     gene            complement(1509..1584)
                     /locus_tag="ACHOQW_004936"
     tRNA            complement(1509..1584)
                     /locus_tag="ACHOQW_004936"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:Ile,seq:cat)
BASE COUNT          376 a          454 c          419 g          359 t
ORIGIN      
        1 tcaccacaaa tccacggcac atctgcagca ctgccaccat ggcactgcgc gttaaacacg
       61 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtgcgtgg
      121 gtggcggcgc tcatgtcaaa ctctccctgc atccagcaca ccgccagcaa cacatttttc
      181 gggttcttct gtaatgcagc tttagtgcgc gcaatcaggt cctgatataa cggtttaccc
      241 acaccccagc gtgccgaatc ctggctggcc cccgtgtccg cactgaatgt cccctcagcg
      301 ccctgggtga atgcagaacc accacgacag catggtacca gcaggatccc cgcgttattc
      361 gggatatacg gaagcagttt tttggcaata tgtaagccct ggccgacaca gccgtactgc
      421 cctttgctca ggtctgcctt cggatgattc agcgtactca tatcctgcac atcatgcagg
      481 cagtggtcgg ccggaatgat gtcgttatac gtacaactct ctccacccgg agttaccgtg
      541 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtaagaatc cggtaacgga
      601 agcccttcac cgtaggccat gccgttggac tgtccggcaa gcacaaccac gtagaaccag
      661 tccggcttag atgaagggcc gacctgtgga tctccttcaa tagccaccgc ctgcatcagt
      721 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      781 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata gtgtttaaat
      841 gccatgaatc ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc
      901 ggaagttaca ggacaatggc agaagagaga caacagcccg caatacgaaa aaggccgcgc
      961 tattgcgcag agtgattact gtcggatatt attcgccagc tgaaatatta cttcacgttt
     1021 tgttgtttat tccttgccgc ccgcgtctcc ctgcgcgggc tttttttgtc cataagaaag
     1081 cccctccgga gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc
     1141 ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt
     1201 gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca
     1261 gacaacagtc aaccgcctga attgtgagac atttaaaaaa aaggcccgca aaagcgagcc
     1321 agggaaaata agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg
     1381 caattgctct gtccggctga gctaacaacg caggatacag ataatggacc gccttcgggg
     1441 acccgaactc cgcgcaacca gcttcgaaag ctggcgctct ttcctgatga gctaatggcg
     1501 gtatgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg
     1561 ctctcaccac tgagctaaag ggctgggtca aaaaataata atcagatg
//
LOCUS       DAZKFZ010000397         1593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000397 DAZKFZ010000000
VERSION     DAZKFZ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..179
                     /gene="espM1"
                     /locus_tag="ACHOQW_004937"
     CDS             <1..179
                     /gene="espM1"
                     /locus_tag="ACHOQW_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFP9575824.1"
                     /translation="KKMDALCDGMSRAVKNSTTDYIANVLADKFYQKHIAPGVDIVKL
                     RNEIPGYMSRVIQG"
     gene            complement(398..1454)
                     /locus_tag="ACHOQW_004938"
                     /pseudo
     CDS             complement(398..1454)
                     /locus_tag="ACHOQW_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          409 a          346 c          386 g          452 t
ORIGIN      
        1 gcaaaaaaat ggatgctctt tgcgatggta tgagccgagc tgtaaaaaac agtacaacag
       61 attacatagc aaacgtgctt gcagacaagt tttatcagaa acatatcgcc ccgggtgttg
      121 atattgtaaa actacgaaat gaaatcccag gttatatgag tcgtgttata caggggtaag
      181 gttggaggag gctatgcgcc agtatcattg gttttcatcc tgtaaaaaga tatgagtaaa
      241 atatacaaca ggcgttacac tatatgtatg tatccaaata actcatagtt cacctgtaca
      301 gttataaatc gtaaagaaaa ttgcagcgcg tcgtacgcag aaacgtgctg tgattgacta
      361 tttatagctt tgtcacaacg acaagattca gtagtgttta cacgggcggg agaaatgacg
      421 gaataactaa atagctgcgc ctaatatcgc acatttttgc cagcccatgt ttgccttcgg
      481 ggaatggact gacggattcc ataaaatggc gaactttttc aatagttgcc acattgagcg
      541 gcactgatga ttacgcgtta ttgtggcgtg aagtatctgc catagctatt caaaatgatt
      601 cacccatgga gattaaaccg gctgataaat gactctgcac ttcggtttat ccttcagcca
      661 gcgctgggtt tcccggcctt tgtggataat gtagttgtcc tcgagcagcg tgatggtttt
      721 cgtccgacag tatgtagctt taagccgctt cagcagtctg atgaacagcg ccgaactttt
      781 gctgttggcg cccacacagc tgacttaacc tgtcccgctg tgcagcgcta cgtccagaga
      841 atatttttca ttttgttccg gcgtgacccc tcgtttttgc ttcccgagca gtttccagtc
      901 cgcaccgatt ttgggattaa gatggataaa tacttcatct tcataaaaga ccggatgctc
      961 tgtgctgcat tcgtccagtg cgttatggat tgttgccatc tttttatctt taggcggggc
     1021 acggagacgt agagttggcg cagcccttcg ccacacaagc cccacagacg acaaccagcg
     1081 gcgaatggtt ccagcatgta acaggcagcc ggttatctca ttgattttta ttgtcagcag
     1141 ttctgtactc cagcgtgaat aattgtagcc aaagtcgcga gtgaatattt tacctgttca
     1201 cgtaatagtg tgtagatatg cgcaaacggc cagcggcggg ctcgctcggc aggtaatgat
     1261 ttcagtccct caacacccga ctgtgtgaac tagttaatcc agcgcccaac agaggaacgg
     1321 gcgcagcata gtgtgcgggc aacgtcgctg acacggtcgc cccggtgcag catcagcatg
     1381 gctgtcagcc ttttatggat ggctttctgt attaggcgtc gttcgtcacg ggaaattggt
     1441 ggtatgatcg acattgctca gtccggttgg tgatttgtat tgatttggcg attgatcaga
     1501 tcgcacaatc gggttgagtt ccctctaaat gatctactat tctgcgcagc tatttagtga
     1561 gtatatattt tatgcttata aattagatta aaa
//
LOCUS       DAZKFZ010000398         1591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000398 DAZKFZ010000000
VERSION     DAZKFZ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..196
                     /locus_tag="ACHOQW_004939"
     CDS             <1..196
                     /locus_tag="ACHOQW_004939"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9575825.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIV
                     RGQLVPGERLGIKITELECA"
     gene            193..882
                     /locus_tag="ACHOQW_004940"
     CDS             193..882
                     /locus_tag="ACHOQW_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9575826.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYKELALLVGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            1093..1168
                     /locus_tag="ACHOQW_004941"
     tRNA            1093..1168
                     /locus_tag="ACHOQW_004941"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1126..1128,aa:Ile,seq:cat)
     gene            1176..1252
                     /locus_tag="ACHOQW_004942"
     tRNA            1176..1252
                     /locus_tag="ACHOQW_004942"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1210..1212,aa:Arg,seq:tcg)
     gene            1266..1342
                     /locus_tag="ACHOQW_004943"
     tRNA            1266..1342
                     /locus_tag="ACHOQW_004943"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1300..1302,aa:Arg,seq:tct)
     gene            1516..>1591
                     /locus_tag="ACHOQW_004944"
     CDS             1516..>1591
                     /locus_tag="ACHOQW_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFP9575827.1"
                     /translation="MVFLVKKARRAATEIKKFEKRDLAQ"
BASE COUNT          430 a          352 c          421 g          388 t
ORIGIN      
        1 gataaagatt attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa
       61 agcaccactg gatgtgctga cgcatgccgg acttctcata gacgacgagc agtttgatga
      121 aatcaatatt gtgcgcggtc agctcgttcc tggtgagcgg ctggggataa aaatcacaga
      181 actggagtgc gcatgaataa ccactattta cagtttgtgc gtgagcagct cattatcgcc
      241 accgctgatt tgagtggggc aacaaaaggt cagcttgaag cctggcagga gaatgccatg
      301 ttcgatacag ggcgttacag gcgaaaaaaa atccggtacc gcgatgaagt gactggaaaa
      361 atgataacgc gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggtgacgtca
      421 attcctctgg tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct
      481 cttgaagaac atcataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa
      541 tatcagatcg cgataacaca gtgggcgtgg aatgaattta atactcaatc cggtaccaga
      601 aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca
      661 gtaaaagcag aactttttgg tggggaaggt tatgaataca aggagctggc attactggtg
      721 ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac
      781 atttttcacc gactggatag tgaggcttta ttgtttgtaa tgagaacgcg ttcaaaacaa
      841 aaggcggcat tttcaaagca aagtgttgca aaagtagatt gaaaggcata tatttcatgc
      901 aaatctgata ttttgccgat tttgtacgtg atggcaaaag caaacaaaac ccgcccacaa
      961 gcgggttttt ttgtgccact tatctcggat agacatggtg aatgcgctgg tggaggaagc
     1021 aagggtaatt tttaaccagg tgattcttga atgcttgcaa cattgatttc gtaacgttat
     1081 tatcctgcgc ccggcccttt agctcagtgg tgagagcgag cgactcataa tcgccaggcc
     1141 gctggttcaa atccagcaag ggccaccatc acataccgcc attagctcat cgggatagaa
     1201 cgccagcctt cgaagctggt ttcgcggggt tcgagtctcc gatggcggtc cattatcggt
     1261 attctgcgtt tttagctcag ccggacagag caattgcctt ctaagcaatc ggtcactggt
     1321 tcgaacccag tacaacgcac cacacttatt ttccctcgct cgcttttgcg gacctttttt
     1381 gtatccgcac cacgcccggc gcataccaac cacagagcct ttcgggggga gcttatggag
     1441 tggtcagtgt gactttctct gtgggcagat cgctcccggg cgttggctca cccacccaaa
     1501 ggaacgtcac gatgtttggt atttttggta aaaaaagccc gcagagcggc aacggaaatt
     1561 aaaaagtttg aaaaacgcga tctggcacag g
//
LOCUS       DAZKFZ010000399         1554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000399 DAZKFZ010000000
VERSION     DAZKFZ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..198
                     /locus_tag="ACHOQW_004945"
     CDS             <1..198
                     /locus_tag="ACHOQW_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9575828.1"
                     /translation="MLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRG
                     CEMRGMVANFGGFLSINKLSQ"
     gene            complement(215..469)
                     /locus_tag="ACHOQW_004946"
     CDS             complement(215..469)
                     /locus_tag="ACHOQW_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9575829.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            579..689
                     /locus_tag="ACHOQW_004947"
     CDS             579..689
                     /locus_tag="ACHOQW_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9575830.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            992..>1554
                     /locus_tag="ACHOQW_004948"
     CDS             992..>1554
                     /locus_tag="ACHOQW_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFP9575831.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEF"
BASE COUNT          452 a          294 c          384 g          424 t
ORIGIN      
        1 atgctggcga acacctgtat gtgggattac cggggagatg aatgcggtta tcacggtcct
       61 gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      121 tgcatgcgcg ggtgtgagat gcgcggcatg gtggctaatt ttggcggttt cctttccatt
      181 aataaacttt cgcagtaatg gattatgccc accatcaggt gggtttttta tcttcgccgt
      241 acatgctgct gagagtctta agaagagcct ccctaaattt ttcagattct tgttgagcaa
      301 aaccttctat tgatcttggt gatttttctt catcaatagc agcttgcaag atcataacta
      361 tttctgaatt taatgagcgt ccgtttttcg atgctcttat ggtcaggtcg cgcttgaggc
      421 tatctggcat tcttacgcta tatggggcta tgtctctaac cttcgtcata cttacaccgt
      481 gataatcaca ttgatatcac agtgtattca aaaaaacttt gacttgatat agtcacagtg
      541 atacgtttgt gattcataat aactcacgcg gtggcgatat ggaaaaagaa gttagcagaa
      601 ttttggtgag aatacctcag tcgctaaaag atgcgattac aggaaaggcc aaagaagaat
      661 gccggtcgtt taactcggaa gtgatgtgat tgctggacag cctgaagaga gaggggataa
      721 cggtatgagt aaagaatgtt gttttttgcg gcattagcga atcagacgct gatcaaacat
      781 acatttactc taaagaaaca ggtcggatgc tgtgtagtga ctgcgtgttt gacatcataa
      841 gatacaagta tcttggatgt tctgccagca ttagcaatat aggtgaagtg tacgaaggga
      901 aagatataac tgatagagca gaaagttgaa gccccaactg ctgtaacagt cagggcttcg
      961 ttatcaacaa atcggcttag gaaatattga catgaaaagt atagcaaagg cacaaaacga
     1021 tttcactatc ttcaaattcg gcgacagtga aatccgcgtc atcaacaagt gcggtgagcc
     1081 gtggtttgta gctaaagatg tttgtgatgc tttagctttg actaactcac gcaaggcgct
     1141 tactgcactt gatgacgatg aaaagggagt aactttaagt tacacccttg gtggtgagca
     1201 gaatctaagc attgtgagcg aatcaggtat gtatacattg gttctgcgct gccgcgatgc
     1261 agtcaataaa ggttcagtcc cgcacaaatt ccgcaagtgg gtaacagcag aagttctgcc
     1321 ttcaattcgc aaacatggcg agtatgtaaa aggaaagaaa accactgttg aggaaagaac
     1381 accgctacgc gatgcagtaa acatgctggt aggaaagaaa ggacttcgca atgacgatgc
     1441 atacaatatg gttcatcagc gttttggtat tgacagcatt gatgagcttt caattgaaca
     1501 aatcccgctt gccgtagagt acatccacag ggtagtgctt gaaggtgaat tcat
//
LOCUS       DAZKFZ010000400         1554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000400 DAZKFZ010000000
VERSION     DAZKFZ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..258
                     /locus_tag="ACHOQW_004949"
     CDS             <1..258
                     /locus_tag="ACHOQW_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9575832.1"
                     /translation="ASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVAD
                     EFDNPTTDIRKDRCSKCMRGCELRRNVGNFGGFLSINKLSQ"
     gene            complement(275..595)
                     /locus_tag="ACHOQW_004950"
     CDS             complement(275..595)
                     /locus_tag="ACHOQW_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9575833.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            703..876
                     /locus_tag="ACHOQW_004951"
     CDS             703..876
                     /locus_tag="ACHOQW_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9575834.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            947..>1554
                     /locus_tag="ACHOQW_004952"
     CDS             947..>1554
                     /locus_tag="ACHOQW_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9575835.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGEL"
BASE COUNT          408 a          344 c          372 g          430 t
ORIGIN      
        1 gcctcgtttg tgctggccac accgaccgag acggacgggg cgctgtttcc cggtcgcatc
       61 atgctggcga acacctgtat gtgggattac cggggagatg aatgcgggta taacggtcct
      121 gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      181 tgcatgcgcg ggtgtgaact gcgcaggaat gtcggcaatt ttggcggttt cctttccatt
      241 aataaacttt cgcagtaatg gattatgccc accgtcaggt gggtttttta tttagtagtt
      301 ctctcaactt ttcgttctgc tctctgaact tttcctgtat ttctttttgc atggcgataa
      361 cctgggcttg aagttgaact agcgcatcaa catttagcgg aaccgagacg ctgttaattt
      421 tatctgctat ttccccgagt gtattttctg cattcaaggc atcttccagt atctgaacaa
      481 tctctgagtt catagagcgc ccgtttcgtt tggctcgttc agctatagca tctcgcattc
      541 cgtcaggaaa acggaggttg aacttgtcgt agtcttttac ttgtttttcg gccattgcaa
      601 atctctcaaa aaaaatcatg gtgccatatt gccatacgat ttcaatggtg gcattatggc
      661 cttcatggcg tcactttggc accaaataaa ggagattaga taatgcaaga tacacttttc
      721 actgagcgca aaaatatcaa actcaacctt cgccttccat cacggctgaa tgaagacctt
      781 cgccgcctgg cggaaatgga ctgtatatct ctgaactctg caattgttcg tttgctggca
      841 aaaggtgtta gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct caatggctgc
      901 aaccattgag gcttctaaat taccagttaa ccacgagaaa actgatatga ctagtttagc
      961 aattgcagat cgcacaatca atgttccatt ccacggaaca aatctctttt tggttggaat
     1021 taacaatgag ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga tggtttgggc
     1081 tgctcaattt gttaagttaa aacagaggtt tgtcaaaggt atttcggaaa tcgaaatacc
     1141 ttctgctggc ggtaaacagt taatgacatg tcttgccttt cgtaaatttg cggcttggct
     1201 ttcaagcatt caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa tccagtatca
     1261 ggaagagtgt gacgatgtgc tctacgagta ctggactaaa ggccatgtga ttaacccgcg
     1321 caaagctaaa aaggcattgc cggggaaaat caccactgaa cagcaggaag ccattaaaca
     1381 actcgtcatg agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg cgatgatcac
     1441 catgtggtcg tcactgaaat ctcattttgg gtgttcatac aaagaaatca gcgaggagca
     1501 gtttaccgaa gctctgtcac ttgctgctcg cgttccgctt gaaggagagt taat
//
LOCUS       DAZKFZ010000401         1539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000401 DAZKFZ010000000
VERSION     DAZKFZ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(71..1477)
                     /gene="gndA"
                     /locus_tag="ACHOQW_004953"
     CDS             complement(71..1477)
                     /gene="gndA"
                     /locus_tag="ACHOQW_004953"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9575836.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          385 a          390 c          374 g          390 t
ORIGIN      
        1 aagataattg gcaaaaaaaa gcccggtaca atatacgccg ggcctcaatt ttattgttga
       61 ttaaatcaga ttaatccagc cattcggtat ggaacacacc ttctttatca atgcgcttat
      121 aagtatgcgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
      181 cgcggtagct gtcataatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
      241 gtaccgcata agcgacgaca tcgcgcagcg cctgctggta gtcatcggca atttgcttga
      301 agtaaggagc cagcagcagg ttagcgatct gcggattttc ggcataagca tcggtgattt
      361 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
      421 agttcagatc ccagttgtac tcttcagacg ccgcacgcag ttgagagaac ccctgagcgt
      481 aagaaacgat tttgcccaga tacagtgcac ggcgaacttt ttcgatgaac tcagccttgt
      541 cgccagctgg ctgcgcttgt gggccagaga gaactttaga cgcagcaacg cgctgatctt
      601 tcagagaaga gatgtatcgt gcaaacacag actcggtaat cagcgacagc ggttcgccga
      661 gatccagtgc gctctggctg gtccatttgc ccgtaccttt gtttgccgct tcatccagga
      721 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatgtct ttggtaatgt
      781 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaagatctgc gccagttctt
      841 cgttggtgag gttcagacca cctttaagca gagaataggc ttcagcaatc agctgcatat
      901 cgccgtattc aataccgttg tgaaccatct tcacatagtg acctgcgcca tcggcaccaa
      961 tataggtaac gcatggctca ccgtcttcag ccactgcggc gattttggtc aggatcggcg
     1021 caactagttc ataggcttct ttctgcccac caggcataat ggaaggacct tttagtgcgc
     1081 cctcctcacc accggaaaca ccggtaccga tgaagttaaa gccttctgca gaaagctcac
     1141 ggttacgacg aatggtgtcc tggaagaagg tattaccacc atcaatgatg atgtcacctt
     1201 tatcgaggta tggcttaagg gaatcaatag cagcatccgt gcctgcacct gctttcacca
     1261 ttaacaagat gcgacgaggc gtttccagag attcaacaaa ttctttcacc gtatagtaag
     1321 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
     1381 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
     1441 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt caggggttac
     1501 cgctagatat gcggcttgaa taacgtataa aaatgtatc
//
LOCUS       DAZKFZ010000402         1535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000402 DAZKFZ010000000
VERSION     DAZKFZ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.406"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(197..393)
                     /locus_tag="ACHOQW_004954"
                     /pseudo
     CDS             complement(197..393)
                     /locus_tag="ACHOQW_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(680..1498)
                     /locus_tag="ACHOQW_004955"
     CDS             complement(680..1498)
                     /locus_tag="ACHOQW_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP9575837.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
BASE COUNT          565 a          356 c          254 g          360 t
ORIGIN      
        1 ttttcaagaa aaatattaat tggggagcaa accatgataa ctgaacagac aacaaccatt
       61 ataattttac tttcattagc aattggttag ttcaattata gccccaaaag gtaaattatc
      121 atcaacacat aagcaaagga ctgacaggtg ccgctaacac ccaccagccg cccatttacc
      181 acaaataaaa aagccttcag gactgaaggc gtctgtaaca accgaactga tagtctgcca
      241 gacccgcata accagctggg tcagtattaa ctggcagcgc tcgcgtgaaa ggtacgtatt
      301 ctgcgcaatc tccccgaccg tcgccggttc ggtgacgctt aattcatcaa acacaactct
      361 ggcggtttct gtcatatcct gctgtttcag catgtctttt taccctttcc ggttaacgtg
      421 acacaccaat aactcttgtc gaaaaagcca gcaagctgaa agacctgtat taataactac
      481 cagcacattt aacgcactgc gctactttgc gggcacaaaa aacccgctca gaggcggggt
      541 caagctatgc ggcgaaataa ccactcttaa cagcatacct gattttttac gtacgtaaat
      601 gctttgccgt gcatattttt catgcaaatg tcacgtcctg ctatttttca gtcttataaa
      661 tttaaaacca tagaaaaaat caattatgtt ttaaaaatgg ataggtaaag aataacaagt
      721 gacacagatt caaaccaaaa tggaaaaggg tggcaaccca caaacgccca ctccacatcc
      781 atgacaatcc atacacaaca ccagataacg tggcaaataa tacaagtaaa gcaccacctg
      841 aatagtgata aaaaccaaac aacaaagccg ccacaattaa tgcaaccaat ggagacgtta
      901 cttctgatag ccgtgattga atataccctc taaataatga ttcctctgcc agagacacaa
      961 aaaacaaatt agccaatata aactctggca accactcagg aaaatgaatc tctggcttta
     1021 atccaccaaa aaaaacagcc aaaaacagga taagaggaac agagagcgac agagcccccc
     1081 acttccacaa agacacttct gattttactt cttttttaaa caaagaagat gtacacagga
     1141 ctaacaaaaa tggcaccagc gctttatcaa aattaaaata cattgtatag ggagtacttt
     1201 gagggccaac agtaacagaa tttagcacta caggattgtg aaaccctggc catagatgga
     1261 aaaataatgc tatggctgat aaaactatgc caacttcata tatagattta gcccaggcgt
     1321 tatatttcca gttgaacttc aaaataataa aaaaaacgat tgtagcaaca aaaaataaca
     1381 ccgaccaatc aataatatca ttaagcacag ccaggacaac agacaccgtc aacaatgaaa
     1441 aagccactac cttatgccaa ctgagagttg acagtgacag tactaacaca atccacataa
     1501 gcactccttt tatttaatga tgaagattga ttatc
//
LOCUS       DAZKFZ010000403         1526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000403 DAZKFZ010000000
VERSION     DAZKFZ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..368)
                     /locus_tag="ACHOQW_004956"
     CDS             complement(<1..368)
                     /locus_tag="ACHOQW_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9575838.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
     gene            complement(378..707)
                     /locus_tag="ACHOQW_004957"
     CDS             complement(378..707)
                     /locus_tag="ACHOQW_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575839.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(707..>1526)
                     /locus_tag="ACHOQW_004958"
     CDS             complement(707..>1526)
                     /locus_tag="ACHOQW_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9575840.1"
                     /translation="QVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLN
                     RQLNENRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLA
                     TFVTTGKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQ
                     SADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMA
                     MFSPQYNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
BASE COUNT          339 a          476 c          330 g          381 t
ORIGIN      
        1 ctgtttccgt tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggacc
       61 accgttccgc cgaccagact ctgcaggtct tccgccatcc ccgtgaccat gccgtgcagg
      121 ttagaaaccg tcagtgtcgg acgggcagaa ctgcccttgc cgttcagttc aaatcccgtc
      181 ccctgaatgg ggtatgcctg atactgccgc ccctgccagg taaccggctc acctttttcg
      241 ttctgctcat tacagaaaaa ataacgttca ccaccgacct ctgtcagatc gatttcccag
      301 agcaccgcct gggctgactg agtgaggcgt gtcgtctcat gatgtgtttc ctgtggaata
      361 tcctgcatca gagcctccta tgccacgacc tgttcaaaat ctgccgttat ggttacccac
      421 agcgccccca cgcttgccga ccatttacga caaaccaccc tgatcggttt ccagtcataa
      481 ggtggcgtcc actgaaatgc acggacgcca ccgtgccgtt ccagaaaggc ttttaaagat
      541 gggtgttcac atttacgaac acgtatcgtc acgctgtaag tcgacaactg gttattcagt
      601 cccgccgcac gacgctgttc ataaccatcg cccagcttca ctgtcaccac tttcggctct
      661 gataccacat tcatatccgg gcgcactttc cagtgaaacg tctccattac cgatatgctc
      721 cacttaaccg accaccatca cgggcctgct gttgcataaa gtccgctgcc gcttttttcc
      781 cgaggtcata aaccgccttc agggcagccg gacctatctg cccgttcgtg ccatcgttat
      841 tgatctcgat gttgtactgc ggggaaaaca tcgccatacc tgaaccccca atatccgcca
      901 caacccccag cttaccgtca gcaccacgac gcaatggcag aatggcttca ggtcccgctt
      961 cccccatcac acccgcgcct tttgcaaaag caaaaaacgt cggacggtta accaccgtgc
     1021 cactgtaacg actcaaatca gcagactgat aaatcccccc attggcattc agggaaaggc
     1081 tgctgagatc aaatcccagt acactgccaa tcccttttat cgatttcatc atggttgcct
     1141 gcgccaggat tttcgccata tctgacagca cagaagaggt gaaggatttg aaattgagtt
     1201 tgccggtagt gacaaaagtt gccaggccat ttcccatgtt gctgaaggct gacgagaaca
     1261 cctgttctgc tgtttctgcc gtattatctg catccacggt aaaatcctga aatgcacgca
     1321 ggactccgtt tttccagtta ccctgtacaa cttcaagctg ttgccagtaa cggcgattct
     1381 cattcagttg tcggttcagg ctctccgtca gcgcctgctc ggcctttctg tagtcatccg
     1441 tgttatatgt ccctttctgc tcactatccc gcctcaactg ctccagctgt tgctggtatt
     1501 tctggcgaag actcagttgt acctga
//
LOCUS       DAZKFZ010000404         1516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000404 DAZKFZ010000000
VERSION     DAZKFZ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            185..>1516
                     /locus_tag="ACHOQW_004959"
     CDS             185..>1516
                     /locus_tag="ACHOQW_004959"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9575841.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKAS"
BASE COUNT          357 a          395 c          391 g          373 t
ORIGIN      
        1 caccttacta ccacccaaga gctccctact atattgtttt tcaatgaaaa aatattattc
       61 acgcaatatc tcaccataaa taacattagg attttgttat ttaaattaag cctgtaatgc
      121 cttgcttcca ttgcggataa atcctacttt tttattgcct tcaaataaat ttaaggagtt
      181 cgaaatggac cagaagctgt taacggattt ccgctcagaa ctactcgatt cacgttttgg
      241 cgcaaaggcc atttctacta tcgcggagtc aaaacgattt ccgctgcacg aaatgcgcga
      301 tgatgtcgca ttccagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
      361 cctggccact ttctgccaga cctgggacga cgaaaacgtc cataaattga tggatttgtc
      421 gatcaataaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
      481 ttgcgtaaac atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt
      541 tggcaccaac accattggtt cttccgaggc ttgtatgctt ggcgggatgg cgatgaaatg
      601 gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa acctggtgtg
      661 cggtccggtg caaatctgct ggcataaatt cgctcgctac tgggatgtgg agctgcgcga
      721 gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg aagcctgcga
      781 cgaaaacacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta actatgagtt
      841 cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta tcgacatcga
      901 catgcacatc gacgctgcca gcggtggctt cctggcaccg ttcgtcgccc cggatatcgt
      961 ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata aattcggtct
     1021 ggctccgctg ggctgcggct gggtgatctg gcgtgatgaa gaagcgctgc cgcaggaact
     1081 ggtgttcaac gttgactacc tcggtggtca gattggtact ttcgccatca acttctcccg
     1141 cccggcgggt caggtgattg cgcagtatta tgaattcctg cgcctcggtc gtgaaggcta
     1201 taccaaagta cagaacgcct cttaccaggt tgccgcttat ctggcggatg aaatcgccaa
     1261 actggggccg tatgagttca tctgtacggg tcgtccggac gaaggcatcc cggcggtttg
     1321 cttcaaactg aaagagggtg aagatccggg atacaccctg tacgacctct ctgaacgtct
     1381 gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca ccgacatcgt
     1441 ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga
     1501 agactacaaa gcctcc
//
LOCUS       DAZKFZ010000405         1498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000405 DAZKFZ010000000
VERSION     DAZKFZ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            297..458
                     /locus_tag="ACHOQW_004960"
                     /pseudo
     CDS             297..458
                     /locus_tag="ACHOQW_004960"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ACHOQW_004961"
     CDS             442..507
                     /locus_tag="ACHOQW_004961"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFP9575842.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ACHOQW_004962"
     CDS             623..880
                     /locus_tag="ACHOQW_004962"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9575843.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHOQW_004963"
     CDS             complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHOQW_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFP9575844.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          448 a          344 c          299 g          407 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt tactttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagctcatta
     1321 ttatttctgg cacatgaata tatagagacc ataacatcaa gtttatcttg taattcagga
     1381 gaaagaaagt gattatgatt aagctcactt actgattccg gagtcgaagg gttacctctt
     1441 cctgatacat acatccatga ataaagattt gctgaagaac cacttgttgg taacatat
//
LOCUS       DAZKFZ010000406         1481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000406 DAZKFZ010000000
VERSION     DAZKFZ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            169..879
                     /locus_tag="ACHOQW_004964"
     CDS             169..879
                     /locus_tag="ACHOQW_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9575845.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSNLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            921..>1481
                     /locus_tag="ACHOQW_004965"
     CDS             921..>1481
                     /locus_tag="ACHOQW_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFP9575846.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFITDDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAG"
BASE COUNT          419 a          348 c          303 g          411 t
ORIGIN      
        1 aaatatttac tttttagcaa ggttttccta ttaaaagaga accaaccttt attacaaacg
       61 cattaagtct ggaaacccgg tgactcgcca atgtattagc aacatttacc gattgtctga
      121 tgtaacggtt ttcccgctta tcctcttaac acatggagaa tgcatcagat gcaaactacg
      181 cgtaccccct actctatttc ctttatggcg acagtgttac ttttattgct ctttgcttgc
      241 catagcaccg ttgccaacgc agctgtcgca ctgggtgcga cgcgggtaat ttatccggca
      301 aaccagaaac aggttttatt gcctgtcacg aataatgatc ctgccagtgt ctatctcatt
      361 cagtcatgga tagaaaatgc tggtgaccaa aaagacaccc agtttgtcat taccccacct
      421 cttttttcca tgcaaggtaa aaaagagaat acgctgcgga taatcaacgc cactaatcat
      481 cagttacccg gcgaccgtga aagcctgttc tgggttaatg ttaaagctat tcctgctatg
      541 gaaaaagacc agaagaatga gaatacatta cagctggcaa ttattagccg cataaaaatg
      601 ttttatcggc ccacgaacct ggcaatggca cctgaagaag cgcctgcaat gcttcggttt
      661 cgtcgcagcg gtagcaatct cactcttatt aatccaacgc cctacttcat taccgtaaca
      721 aatatgaaag caggaaatag caatctccca aacaccatgg tgccgccaaa aggtgaagtg
      781 tcggtggata tcccgcatgc tgccaccggg gatatcagtt ttcagacgat caatgattac
      841 ggcgcattaa ctccgcgtat taaggcaacc atgcagtaag ccagtgacct aattaataat
      901 aaactcaatc ggaattaata atgacagcct ttcgtgccgc atttaaggca tatcgtatgc
      961 atcaggtgtt actactgcct cgctttgcac ggcttaccat tgcccttagc ttagcaactg
     1021 cggtatttcc cgttgacgca gagtattatt ttaatccacg gtttttaagc aatgatcttg
     1081 ctgaatctgt cgatttatcc gcctttacta agggacgcga ggcaccacct ggtacatatc
     1141 gggtcgacat ttatttgaac gacgaattta tgactagtcg ggatattact tttattacag
     1201 atgataataa cgcagatctc atcccatgcc tgagtacaga tcttcttgtc agtcttggca
     1261 taaaaaaatc tgcgttatta gacaataagg aacattcagc agaaaaacat gtgccggaca
     1321 acagcgcatg cacaccactt caggatcgtt tggtcgacgc atcaactgag tttgatgttg
     1381 gtcagcaaca tctgtcactc tccgttccgc aaatttatgt tggcagaatg gctcgcggct
     1441 atgtttctcc tgatctgtgg gaagaaggaa taaatgctgg g
//
LOCUS       DAZKFZ010000407         1477 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000407 DAZKFZ010000000
VERSION     DAZKFZ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            172..732
                     /locus_tag="ACHOQW_004966"
                     /pseudo
     CDS             172..732
                     /locus_tag="ACHOQW_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            940..1296
                     /locus_tag="ACHOQW_004967"
     CDS             940..1296
                     /locus_tag="ACHOQW_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575847.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
BASE COUNT          420 a          345 c          305 g          407 t
ORIGIN      
        1 cattttcctt catcggactg taaattgcgg agatatcgaa cttgcactac ccatcgcaac
       61 actcaaacag atttatgaat ttcgttcata acccctgaca aatcctgccg tctaatcgag
      121 ctcttctctt tacgttatgc ttctcatgaa cgataacgga aggataacac catgcaaaaa
      181 gtaaaactga ataacggtat tgaaatgccc ctgctgggct ttggtgtatt tcagatgacc
      241 gatgccgccg aatgcgaaag agcggtcatt gacgctatta acaccggtta tcgactgatt
      301 gacaccgccg cctcctatca aaatgaaccc caggtcggga acgcactgaa acaaagcggt
      361 atcgcccgta acgaactctt tgtcaccacg aaactctggt tgcaggatac cagttacgaa
      421 ggggccaaag cccagttcga acgctctctg aatcgactgc aacttgatta cgttgacctg
      481 tatttgattc atcagcctta cggtgatgtc cacggtgcct ggcgtgccat ggaagagctg
      541 caacaggccg gtaaaattcg cgctattggc gtcagtaact tccatcctga ccgactggct
      601 gaccttatcg cctttaataa cgtcgttcca gcggtaaacc aggttgaagt taaccctttc
      661 aatcagcagc tacacgccgt tccgtggatg caaagccgtg gtattcagcc agaagcctgg
      721 gcaccgtttg cagttacagg gcggtaagcg attatgcatc tacctattta cgtcggtcac
      781 tcagtcagag tcttgcccat attcaatgag gttcaacacc gcagccaatg aaaagtaggc
      841 catgcgggga ttaatagcat tactcattaa ccaatcgctc ttttcattta cgtgcttttt
      901 atgtaacata ttgcttttat tgatcttcct ggagtttgca tgaataagaa tctcatcctc
      961 gcatttgcat tattttcctt acctgtattt gcagaagaag atctggggcc aggcaaatat
     1021 gtttgtgaca ttaggatttc cagcctggat accgctactc aaattctgtc aaaatccgca
     1081 acagttctcg ataatggaaa caatttcatc gttcaaatgc caaatggaga tcaattgtac
     1141 tctccagatc tggaaaatgt tgatgatggt ataaaacaaa aagcaacaat aggtggagtg
     1201 acatttattc gtcgtccaac ttttaacgat cgcttcatcg tggaagatgg aaatacagga
     1261 ttcttctata agatgcgaaa ctgcgagaag aaataaagga agtttgatag cgatatcctc
     1321 ctgatttcag gctttgtacc tgttcaagac ttacaactac aagccgctac gtgttaacgc
     1381 ctcccaaaat aaccaccgtt aaccgtagcg gtcatagata ctattatttt aaccacatta
     1441 ctggtgataa tgataattga tattatggcc gtataga
//
LOCUS       DAZKFZ010000408         1436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000408 DAZKFZ010000000
VERSION     DAZKFZ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..988
                     /locus_tag="ACHOQW_004968"
     CDS             <1..988
                     /locus_tag="ACHOQW_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9575848.1"
                     /translation="PENSVDLIVTDPPYFKVKPEGWDNQWTGDEDYLKWLDQCLAQFW
                     RVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNHIIWAKPSGRWNGCNKESLRAYFPA
                     TERILFAEHYQGPYRPKDAGYEAKGRALKQHVMAPLISYFRDARAALGITAKQIVDAT
                     GKKNMVSHWFSASQWQLPNESDYLKLQALFARVAEEKHQRGELEKPHHQLLETYTSLN
                     RQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASS
                     RPGDLVADFFMGSGSTVKAAMALGRRATGVELETERFEQTVREVQDLVSQNG"
     gene            1034..1104
                     /locus_tag="ACHOQW_004969"
     tRNA            1034..1104
                     /locus_tag="ACHOQW_004969"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1062..1064,aa:Met,seq:cat)
     gene            complement(1208..1342)
                     /locus_tag="ACHOQW_004970"
                     /pseudo
     CDS             complement(1208..1342)
                     /locus_tag="ACHOQW_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          345 a          327 c          436 g          328 t
ORIGIN      
        1 acccgaaaat tctgttgacc tgatagtcac ggacccgccg tactttaaag tgaagcctga
       61 gggctgggat aaccagtgga cgggtgatga ggattacctg aaatggctgg accagtgtct
      121 ggcgcagttc tggcgggtgc tgaaacctgc cggaagtctt tacctgttct gtggccatcg
      181 cctggcatct gacattgaaa tcatgatgcg tgaacgcttc agtgtgctga accatattat
      241 ctgggcgaag ccgtccggac gctggaacgg gtgcaacaag gaaagcctga gggcgtattt
      301 ccccgccaca gagcgcattc tgttcgcgga acattatcag gggccgtatc gtccgaaaga
      361 tgccgggtat gaggcgaagg gcagggcact gaaacagcat gtgatggctc cgctgatttc
      421 ttactttcgt gatgcgcgtg ctgccctggg gataacggca aaacagatag tggatgccac
      481 aggaaagaaa aacatggtgt cgcactggtt cagtgccagt cagtggcagc taccgaacga
      541 aagcgattat ctgaaattac aggcgctgtt tgcccgggtg gcagaagaga agcatcagcg
      601 cggtgaactg gaaaagcccc accaccagct gctggagacg tatacttcac tgaaccggca
      661 gtatgcggaa ctgcagagtg aatataaaca tctgcggcgg tattttggcg tgacggcgca
      721 ggtgccgtac acggatgtgt ggacgcataa accggtgcag tactatcccg ggaaacatcc
      781 gtgcgaaaaa ccggcagaaa tgctgcagca gataatcagt gcgagcagtc gtccgggtga
      841 cctggttgca gatttcttca tggggtcggg ttcgacagtc aaagccgcga tggcgctggg
      901 gcgtcgtgca actggcgttg agctggagac tgaacgtttt gagcagacgg tcagggaagt
      961 acaggattta gtcagtcaga acggatgata ttgcaggatt agttacgtac cgttattatc
     1021 ctgcgcccgg ccctttagct cagtggtgag agcgagcgac gcataatcgc caggtcgctg
     1081 gttcgaatcc agtacaacgc gccacactta ttttccctgg ctcgcttttg cgggcctttt
     1141 tttaaatgtc tcacaattca ggcggttgac tgttgtctgg tttgcgggga gtttgttaaa
     1201 agaaactggc atggtgaatc cccctgtgcg gaggggcaat cagcaaccag gtatatgggg
     1261 taatcgcgga ttcaggtgct ggtactgaat tcaccgggag gcacccggca ccatgcaaga
     1321 aaaagaatgt gcatgcaaac atgcccctct ccggaggggc ttttttatgg gtaaaaaaat
     1381 gcccgaatgg gttcgggcaa tagcatgaga tactgatatt gttgtgttgt tatcgt
//
LOCUS       DAZKFZ010000409         1416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000409 DAZKFZ010000000
VERSION     DAZKFZ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..1351
                     /gene="cpsG"
                     /locus_tag="ACHOQW_004971"
                     /gene_synonym="manB"
     CDS             <1..1351
                     /gene="cpsG"
                     /locus_tag="ACHOQW_004971"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9575849.1"
                     /translation="KAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTS
                     EALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKL
                     VREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYINVKN
                     LTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPEC
                     RDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKI
                     IHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFAYC
                     DSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSR
                     EALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKALLKLLSE"
BASE COUNT          352 a          358 c          391 g          315 t
ORIGIN      
        1 taaagcctat gatattcgcg ggaaattagg cgaagaactg aatgaagata ttgcctggcg
       61 cattgggcgt gcctatggcg aatttctcaa accgaaaacc attgttttag gcggtgatgt
      121 ccgcctcacc agcgaagcgt taaaactggc gcttgcgaaa ggtttacagg atgcgggcgt
      181 cgatgtgctg gatatcggta tgtccggcac cgaagagatc tatttcgcca cgttccatct
      241 cggagtggat ggcggcatcg aagttaccgc cagccataac ccgatggatt acaacggcat
      301 gaagctggtg cgcgaagggg ctcgcccgat cagcggtgat accggactgc gcgatgtcca
      361 gcgtctggca gaagccaatg acttccctcc tgtcgatgaa accaaacgtg gtcgctatca
      421 gcaaatcaat ctgcgtgacg cttacgttga tcacctgttc ggttatatca acgtcaaaaa
      481 cctcacgccg ctcaagctgg tgatcaactc cgggaacggc gcagcgggtc cggtggtgga
      541 cgccattgaa gcccgattta aagccctcgg cgcaccggtg gaattaatca aagtacacaa
      601 cacgccggac ggcaatttcc ccaacggtat tcctaacccg ctgctgccgg aatgccgcga
      661 cgacacccgt aatgcggtca tcaaacacgg cgcggatatg ggcattgcct ttgatggcga
      721 ttttgaccgc tgtttcctgt ttgacgaaaa agggcagttt atcgagggct actacattgt
      781 cggcctgctg gcagaagcgt tcctcgaaaa aaatcccggc gcgaagatca tccacgatcc
      841 acgtctctcc tggaacaccg ttgatgtggt gactgccgca ggcggcaccc cggtaatgtc
      901 gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag gaagacgcca tctacggtgg
      961 cgaaatgagc gctcaccatt acttccgtga tttcgcttac tgcgacagcg gcatgatccc
     1021 gtggctgctg gtcgccgaac tggtgtgcct gaaaggaaaa acgctgggcg aaatggtgcg
     1081 cgaccggatg gcggcgtttc cggcaagcgg tgagatcaac agcaaactgg cgcaacccgt
     1141 tgaggcaatt aatcgcgtgg aacagcattt tagccgcgag gcgctggcgg tggatcgcac
     1201 cgatggcatc agcatgacct ttgccgactg gcgctttaac ctgcgctcct ccaacaccga
     1261 accggtggtg cggttgaatg tggaatcacg cggtgatgta aagctaatgg aaaagaaaac
     1321 taaagctctt cttaaattgc taagtgagtg attatttaca ttaatcatta agcgtattta
     1381 agattatatt aaagtaatgt tattgcggta tatgat
//
LOCUS       DAZKFZ010000410         1400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000410 DAZKFZ010000000
VERSION     DAZKFZ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            42..656
                     /gene="evgA"
                     /locus_tag="ACHOQW_004972"
     CDS             42..656
                     /gene="evgA"
                     /locus_tag="ACHOQW_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP9575850.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            661..>1400
                     /locus_tag="ACHOQW_004973"
     CDS             661..>1400
                     /locus_tag="ACHOQW_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9575851.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTR"
BASE COUNT          444 a          294 c          268 g          394 t
ORIGIN      
        1 acagggagaa gggagatgct tcattgcaaa gggaataatc tatgaacgca ataattattg
       61 atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa
      121 tcttagcaga gttgactgaa ggcggaagtg ccgttcagcg ggtggaaaca cttaagcctg
      181 atatcgtcat cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc
      241 tgaggaagcg ccaatatagc ggaattatta ttatcgtctc cgctaaaaat gaccattttt
      301 acgggaaaca ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga
      361 acaatatcat tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc
      421 tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac
      481 aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac attgctgaaa
      541 aaatgttcat cagcaacaaa actgtcagca cttataaaag tcgcctgatg gaaaaattag
      601 aatgtaaatc actgatggat ctttacacat tcgcacaacg taacaaaatc ggctaaccac
      661 atgaagtttt taccctatat ttttcttctc tgttgtggtc tttggtcgac cataagtttc
      721 gcagacgaag attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat
      781 ccactacgtc tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt
      841 gcagtacaca agtcccaaac ggctacgttg ttgcataccg attcgcagca acgggttcgt
      901 ggtattaatg ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc
      961 cgggaatacg cagatcatca aaaagcaatg gacgcgctgg aagaaggtga agtcgatata
     1021 gtgttatcac atttagttgc ttcgccgcct cttaatgatg acattgctgc aaccaaacca
     1081 ctgataatta cctttccggc gctggtcacc acccttcacg attcaatgcg accgcttacc
     1141 tcatcaaaac cagtaaatat tgctcgagta gcaaattatc cccctgacga ggtaattcat
     1201 caatcatttc caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
     1261 gtctcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt
     1321 tcccgctatt tcactcactc cttaaatgta gtgaaatatt ataactcacc gcgtcaatat
     1381 aacttcttct taaccagaaa
//
LOCUS       DAZKFZ010000411         1400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000411 DAZKFZ010000000
VERSION     DAZKFZ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            78..>1400
                     /locus_tag="ACHOQW_004974"
     CDS             78..>1400
                     /locus_tag="ACHOQW_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9575852.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIARYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     VALSVAPEVTAIAEKIRLLDKELRRALVSLKTLKSKGVNSFSDFYAIDL"
BASE COUNT          484 a          202 c          296 g          418 t
ORIGIN      
        1 tctttttaaa taaaaattaa acaaaaatta tatctcacca ctaaggttta taaaagcata
       61 agttagcagg tgtcaccatg aaaaaagcca tagcatatat gcgattttca tcaccaggtc
      121 agatgtctgg cgactcatta aaccgacaga gaagacttat tgctgaatgg ttaaaggtaa
      181 atagtgatta ttatcttgat accataacat atgaagattt aggattaagt gcattcaaag
      241 gaaagcatgc acaatcagga gctttttcgg aatttttaga tgctatagag catggttata
      301 tattgccagg aactacattg ttagttgaaa gtctggacag actttcaaga gaaaaagtcg
      361 gtgaagcgat tgaacgtctg aaattgattt tgaatcacgg tattgatgtt ataactcttt
      421 gcgacaatac agtctataat attgactctt tgaatgagcc atattcatta ataaaagcca
      481 tacttatagc acaaagggca aatgaagaaa gcgagataaa gtcaagtcgg gttaaattat
      541 catggaagaa aaaacggcag gatgcactgg aatcaggtac gattatgacg gcgtcttgtc
      601 cgagatggct ctccttagat gacaaaagaa cggcttttgt tccagacccc gacagggtga
      661 aaactattga gctaattttt aaactcagga tggaaaggcg ctcattgaat gcaatagcca
      721 ggtatttaaa tgatcatgct gtaaagaatt tctcaggaaa agaaagtgca tgggggcctt
      781 ctgtaattga aaaattatta gcgaataaag ctctgatagg tatatgcgta ccttcatatc
      841 gtgcaagagg gaaagggata agtgaaatcg ctggctatta tcccagagtc atatcagatg
      901 atttgtttta cgctgtacag gaaattcggt tggcaccttt tggtattagc aatagtagca
      961 agaatcctat gctaataaat ctacttcgaa cagttatgaa gtgtgaggct tgtggtaata
     1021 ccatgattgt tcatgcggta tctggaagtt tgcatggcta ttatgtttgt ccgatgagaa
     1081 gattacatcg atgtgacagg ccatcaataa agagagattt ggttgattat aatatcatta
     1141 atgaattgct ttttaattgt agcaaaattc aaccagttga aaacaagaaa gatgctaatg
     1201 aaactttaga gttaaaaatt attgagcttc agatgaaaat taataattta atcgttgcat
     1261 tgtctgtcgc gcctgaagtt accgctatag cagagaaaat aagactatta gataaggaat
     1321 tacgaagagc tttggtatca ttgaaaactt tgaagagtaa aggtgtaaat tcattcagtg
     1381 atttttatgc tattgactta
//
LOCUS       DAZKFZ010000412         1398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000412 DAZKFZ010000000
VERSION     DAZKFZ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..687
                     /locus_tag="ACHOQW_004975"
     CDS             <1..687
                     /locus_tag="ACHOQW_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575853.1"
                     /translation="NNAIDNDDGTMSMEIVMGKHSGKKSYERWVQGAKNRTVLFHYKN
                     LITDLNQAHSCRASLGAYCPFHGEILRKLDQRNEVQRVKLSIDVPFLEGLTPSELYKI
                     RTDYESSFNAFRSVLREAAYDIEAESDPYKRQLINQHFVERIWDEGLTDIEQKIQTYK
                     RKSNKELFLNIAPSLIGFIGAPNPVSLATGAFTILKEVFSITSHSSEIKSHPSYFLLM
                     TSKGKNKFYD"
     gene            complement(824..1342)
                     /locus_tag="ACHOQW_004976"
     CDS             complement(824..1342)
                     /locus_tag="ACHOQW_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575854.1"
                     /translation="MTILEEMEAEAWHILTTIYTYQRLMEGLTNSSTRYELANQLVAL
                     NALLEMLVIRLARLADKRKDVRSVSMFLKRGSYSASPEAVKLAAEKFLALTEPVLKIR
                     YEQIAHMKPGTLSSFEPRELPNEALRATEALIDLIDIARGHPQSYTYRVGSQEPAINL
                     RASVETSELVKI"
BASE COUNT          430 a          277 c          295 g          396 t
ORIGIN      
        1 aataatgcca ttgataatga tgatggtacc atgtctatgg aaattgtcat gggaaagcat
       61 agcggaaaaa aaagttatga acggtgggtg cagggagcaa aaaatcgtac agttctcttt
      121 cattataaaa accttattac tgatcttaac caagcgcatt catgcagagc gtccctgggc
      181 gcatattgcc catttcatgg agaaattctt cgtaaactgg atcaaaggaa tgaagttcag
      241 agggttaaac tctctattga cgttccattt ctagaggggc ttactccaag tgaactctac
      301 aaaattagaa ctgactacga atcgagtttt aatgccttcc gctctgtgct acgtgaagct
      361 gcgtatgaca tagaagcaga aagtgaccca tataagaggc agctgataaa tcagcatttt
      421 gtcgagcgca tatgggatga aggattgacc gatattgagc aaaaaataca gacctataag
      481 cggaaaagta ataaagaact ttttcttaac attgcgccat cattgatagg ttttattggg
      541 gcaccaaatc cagtgtcgtt ggctacagga gcctttacga ttctaaaaga ggtttttagt
      601 ataacaagcc actcttcgga aataaagagt cacccttcat atttccttct catgacgtca
      661 aaaggcaaaa ataaatttta tgactgaagc agtaggaaat attgtgaaaa tatattcact
      721 taagcaaaaa ggttgagggg ctgccggatc tgttattaac ggggagctaa ttactttgcc
      781 gagtaaagca actcggcaaa gtagcataga cttgagtgaa ttattagatt ttaactagtt
      841 cgctagtctc tactgaagct ctcaaattaa tggcaggctc ctgtgagcca actcggtatg
      901 tgtaagactg agggtgacct ctggcaatgt caatgagatc gattagagcc tcagttgctc
      961 tcagagcttc atttggaagt tctcgaggct cgaagctact gagtgtgccg ggcttcatat
     1021 gtgcaatctg ctcataccta atctttaata ctggttctgt gagtgcaagg aacttttcag
     1081 ctgccaattt cacagcctct ggtgaagcag aatacgagcc gcgtttcaaa aacattgata
     1141 cagatctcac atctttcctt ttatccgcaa gacgcgctaa acggatcacc aacatttcca
     1201 acaatgcatt gagggctacc aactgattgg caagctcata tcttgtactg ctattagtaa
     1261 ggccttccat caaccgctga tacgtataga tggttgttag gatatgccat gcttctgctt
     1321 ccatttcttc aagaatcgtc ataaatactc cacaatggtc tgatagttat catgcagata
     1381 tctaaacaac caatttaa
//
LOCUS       DAZKFZ010000413         1398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000413 DAZKFZ010000000
VERSION     DAZKFZ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1398
                     /gene="rhsB"
                     /locus_tag="ACHOQW_004977"
     CDS             <1..>1398
                     /gene="rhsB"
                     /locus_tag="ACHOQW_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFP9575855.1"
                     /translation="ASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIT
                     RYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVH
                     REEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGK
                     AICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHD
                     LTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRV
                     TVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLT
                     YGSGWLAGMKLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQYD
                     RDYTWNDNGELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLPD
                     PELHPDSTLSMWPDN"
BASE COUNT          335 a          439 c          409 g          215 t
ORIGIN      
        1 gggcatcggc gttttactat aaccaccaca gccagttaac gtcagccacc gggcctgacg
       61 ggctggaaat acgccgggaa tatgatgaat ggggccgtct gattcaggaa actgcccctg
      121 acggcgatat cacccgctac cgttatgata atccacacag tgacttaccc tgcgcaacgg
      181 aagatgccac cggcagccgg aaaaccatga cgtggagccg ttacggtcag ttgctgagct
      241 tcaccgactg ttccggttat gtaacccgtt atgaccatga ccgcttcggg cagatgacgg
      301 cggtgcaccg cgaggaaggg ctgagtcagt accgcgcata cgacagccgt ggacagttaa
      361 ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga atacaacgcc gccggtgacc
      421 tgaccaccgt cattgccccg gacggcagca gaaacgggac acagtacgat gcgtggggaa
      481 aagccatctg taccacgcag ggcggtctga cgcgcagtat ggaatacgat gctgccggac
      541 gggtcatccg cctgaccagt gaaaacggca gccacaccac cttccgttac gatgtactcg
      601 accggctgat acaggaaacc ggctttgacg gccgcacaca gcgttatcac cacgacctga
      661 ccggcaaact tatccgcagc gaggatgagg ggctggtcac ccactggcac tatgacgaag
      721 cagaccgcct cacgcaccgc accgtgaagg gtgaaaccgc agagcgctgg cagtatgacg
      781 aacgcggctg gctgacagac atcagccata tcagcgaagg gcaccgggtg acggtgcatt
      841 acgggtatga tgagaaaggc cggctgaccg gtgagcgtca gacggtgcat cacccgcaga
      901 cggaagcact gctctggcag catgagacca gacacgctta caacgcgcag gggctggcga
      961 accgctgtat accggacagc ctgcccgccg tggaatggct gacctatggc agcggctggc
     1021 tggcaggcat gaagctcggc gacacaccgc tggtggattt cacccgcgac cgcctgcacc
     1081 ggaaaacgct gcgcagattc ggccgttatg aactcaccac cgcttatacc cctgccgggc
     1141 agttacagag ccagcacctg aacagcctgc agtatgaccg cgattacacc tggaacgaca
     1201 acggcgaact catccgcatc agcagcccgc gccagacccg gagttacagc tacagcgact
     1261 ccggcaggct gaccggcgtt cacaccaccg cagcgaatct ggatatccgc atcccgtatg
     1321 ccacggaccc ggcaggtaac cgcctgcccg acccggagct gcacccggac agcaccctca
     1381 gcatgtggcc ggataacc
//
LOCUS       DAZKFZ010000414         1392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000414 DAZKFZ010000000
VERSION     DAZKFZ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(314..549)
                     /locus_tag="ACHOQW_004978"
                     /pseudo
     CDS             complement(314..549)
                     /locus_tag="ACHOQW_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(708..1358)
                     /locus_tag="ACHOQW_004979"
     CDS             complement(708..1358)
                     /locus_tag="ACHOQW_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9575856.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCTITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
BASE COUNT          429 a          319 c          233 g          411 t
ORIGIN      
        1 ttcgtatgta ctacggcgtc ccaaccatgt cccaaaacaa aaatttctgc cctatacacg
       61 ttccaaaaaa ttcaacagca aagagagtct gctgacgagt tgttgattca ctgattaata
      121 cgtaaaattg acaactgctg gaaatcatac aatactcgta ctttcgaaag ttcgctaacc
      181 aggtgctaca cgcgaggtgt tacagttttc tttacgattt atcattcaat cctaatccat
      241 gcatgacatg tggtgcctgt ggggtgatcc tacctacgta atgtggacac agccctaatc
      301 aaggttctgt ttttcaaaac agttccgtgc tgagacagcc acaagtactg tgtcggcacc
      361 accgattgta atcacactag atctaattaa acacagccgt ctgcattatt tcccgactca
      421 taaaatatta gccgtaaatg cattcgactt ttagtgagtg gaagaaactt tccgccctag
      481 cattatcgta acagcgcccc tttgcactca tcctgtcatg caggtatgcc gcttcagaag
      541 cgcctgataa tgcgttgaaa tcaggacggt ggctacttgc tattcagttc acgatacatc
      601 ccttaaggac cagcgaatag caatgagtca ctttattatc aagtttgatg accgctttga
      661 tgctaacttt taataagagt ctcttcacac aaaatcgtgt accgagtcta cactgaataa
      721 caatcactcc tcaccgtaaa gtgaccgata ctgcaatcaa actcacactg ctctctggcc
      781 acaagcattg ataatgttat tggctctctg cttagtgggt gatgcatccc atcattaatt
      841 atacgagaaa gagccatttc atcatataaa ctacatactc tggaaccttc agcatttctt
      901 aaaaaaacac ctcgttgagg aattgccagc gtaatcgtgc actgtaatgc ttcatctatg
      961 taagacattt cttcaatatt aactaaaaat gaacatgaat gtaatcgccg ctgtaacaat
     1021 tcaattctgt tatcattact atgcataaat tcagaaataa cctgccccat atatgtactg
     1081 aatacatcaa gaaatgaatt gcctccattc aattgatttt caacagaatc agccaatcta
     1141 atgatatcgg ctagttctgt atggcttaaa cccgaatata gttccccttg aacaggatct
     1201 acaataaaag cgtccatagc actctgatag cgaacagaaa acgatctttc tcctattgtg
     1261 agtctttcca tttgatgatc tctggtcgca cgttgtaaaa cttcaacatc ttgtgttgac
     1321 aagtaatttc cagaagacaa attaaatgaa aatggcataa ttttatccct aaagcatata
     1381 tcttactttt cg
//
LOCUS       DAZKFZ010000415         1389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000415 DAZKFZ010000000
VERSION     DAZKFZ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..306)
                     /locus_tag="ACHOQW_004980"
     CDS             complement(<1..306)
                     /locus_tag="ACHOQW_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9575857.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQVVINAAYLVACADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKTGRRAALREIEDVKHDTRE
                     "
     gene            complement(476..552)
                     /locus_tag="ACHOQW_004981"
     tRNA            complement(476..552)
                     /locus_tag="ACHOQW_004981"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Arg,seq:tct)
     gene            complement(653..728)
                     /locus_tag="ACHOQW_004982"
     tRNA            complement(653..728)
                     /locus_tag="ACHOQW_004982"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(693..695),aa:Ile,seq:cat)
     gene            complement(757..>1389)
                     /locus_tag="ACHOQW_004983"
     CDS             complement(757..>1389)
                     /locus_tag="ACHOQW_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9575858.1"
                     /translation="GSVVLATKCIRELFLMHYGKVKIVDISESVVSQYLESQHKLTRT
                     RLTDIPLYLLLEPNNPALAAALITSQGFSGEVTDMFLMMACLSLFETDERMSLFLSGC
                     LSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMRV
                     AVNMLCSRYGYGQAVAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          365 a          368 c          311 g          345 t
ORIGIN      
        1 ttcacgcgta tcgtgtttca catcctcaat ctcacgtaac gccgcacgac gaccagtttt
       61 aaagttcgta tccagctgac cgataatggt tgcgctaatc gcattaattt ctgacgtaaa
      121 cgcggacagc gcaggctgat tacgcagtac ctgttcgatc ttcgctttct cggatgcctc
      181 acattcacca tctgcacagg ccaccaggta tgcggcgtta atcaccacct gtgccagatc
      241 gcgtttttca aactttttaa tttccgttgc cgctctgcgg gttttctttt tgaagattcc
      301 aaacatcgtg acgttccttt gggtgggtga gccaacgccc gggagcgatc tgcccacaga
      361 gaaagtcaca ctgaccactc cgtaagctca cccccgaaag gctctgtggt tggtatgcgc
      421 cgggcgtggc gcagatacaa aaaaggcccg ccgaagcgag cctggaaaat aagtgtggcg
      481 cgttgtagtg gagtcgaacc actgaccgat tgcttagaag gcaattgctc tgtccggctg
      541 agctaacaac gcagaatacc gataatggac cgccatcggg gacccggccc cgcaccaaca
      601 accctgttat ggtgtcgtct gctcttcctg ataagctaat ggcggtttgt gatggtggcc
      661 cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct
      721 aaagggccga gccaaaaaat aataatcaga tgaaaatcaa taatcaagcc cttgcctgga
      781 tacatatctg tctggcggga agccataata gcggtgaaat acagaaataa agtaggaccg
      841 gcttgaataa ccacattttt ctgctacagc ctgtccatat ccataccggg aacataacat
      901 atttacagca acacgcatcc gctcttccag caacaagcga ctgaacatgc ccccttcatt
      961 tttcagtttt gtctttaaca aactctcact catatgcaac tgtagagcaa tcgcaccaag
     1021 cgtccagctt gctgatatat ctgtctgaat tatcgccctg actttggcac ttatgctgga
     1081 taaacatcca cttaaaaaca atgacatccg ttcatctgtt tcaaacagag acaggcaggc
     1141 catcataaga aacatatccg tgacctctcc ggaaaatccc tggctggtaa ttaaagccgc
     1201 agccaacgca ggattgttgg gttccagcaa caggtaaagc ggaatgtcag tcagacgagt
     1261 cctcgtcagc ttatgctgac tttccagata ttgacttacg acggattcgc ttatatcgac
     1321 aattttaact ttgccataat gcataaggaa aagctccctg atgcatttgg tggccagaac
     1381 gactgagcc
//
LOCUS       DAZKFZ010000416         1379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000416 DAZKFZ010000000
VERSION     DAZKFZ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..1378)
                     /locus_tag="ACHOQW_004984"
     CDS             complement(<1..1378)
                     /locus_tag="ACHOQW_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFP9575859.1"
                     /translation="MAMLIIPLPTYLIDFLIGLNLTLAILVFLGSFYVDRILSFSSFP
                     SILLITTLFRLALAISTSRLILLEADAGEIITSFGEFVIGDSLVVGFVIFSIVTIVQF
                     IVITKGSERVAEVAARFSLDGMPGKQMSIDADLRAGIIDADLAKERRSVLERESQLYG
                     SFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQNGMDFSTALTVYTILTVGDGLVSQ
                     IPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLSNSFVIVVTCVLALSIGLLPGFPLL
                     VFLCLAVILGIYFYFKFKKKGTEETVVEGDITVGLEPYNQDDDISLGIINKLDQVITE
                     TVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYGIRIPGIVIREGEGLNDEDVILMLN
                     EVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIVNSNGEQYYWVTKSDAQKLTKIGCY
                     TRTAMDEMY"
BASE COUNT          483 a          301 c          219 g          376 t
ORIGIN      
        1 tatacatttc atccatggcc gtgcgagtat aacagccaat ttttgttaat ttttgagcat
       61 cacttttcgt aacccaataa tattgttcac cattactatt tacaatgaca ggtttcttga
      121 tcaaagtcga aaccacttca tctgaatatt caaccaacaa gacaagatca tgataaattt
      181 taaattgtga agctctgact tcatttagca ttaatatgac atcttcatca ttaagacctt
      241 ccccctcacg aatgacaata ccgggaatgc ggataccata ctcaataaaa aactgactac
      301 gaatcctatc agcaagattg atttcagtgt attttttagc ctgtacacta ttcataatca
      361 agaccaaggg taccgtttcg gtaataacct gatcaagttt attaatgata cctaatgaga
      421 tatcatcatc ttgattatag ggttccaacc caacggtaat atctccctca acaaccgttt
      481 cttcagtccc tttctttttg aatttaaagt aaaagtaaat tcctaagatt actgccaaac
      541 ataaaaacac tagaagggga aaacctggaa gcaacccaat acttagcgcg aggacacatg
      601 tcacaacaat gacaaaagaa ttacttaata actgggacat tatattttgc cccatattat
      661 cactatcgcc atttacgcgc gtaacgataa atcctgcgct aatagcaatt aataaagctg
      721 gaatttgaga aacaagacca tcaccgacag taagaattgt gtagacagtc aacgctgttg
      781 agaaatccat tccattttgg cctactccta ctgatagccc accgataata ttaacaaaga
      841 taataataat gtttgcaata gcatcacctt tgataaattt catcgcacca tcaaaggaac
      901 catacaactg gctctctcgt tctaatacac tacgacgttc ttttgctaaa tctgcatcaa
      961 tgatcccggc gcgtagatcg gcgtcaatgc tcatttgttt acctggcata ccgtcaagcg
     1021 aaaagcgggc agcaacttca gccacacgtt ccgaaccttt agtaatcacg ataaactgaa
     1081 caatagtaac aatggagaaa ataacaaaac caactaccag gctatctcca ataacgaact
     1141 caccaaaact cgttataatt tctccagcat ccgcctcaag aagtataagt cgactggtac
     1201 tgatcgccag cgccaaacga aataacgttg tgataaggag gattgatgga aatgaagaaa
     1261 agcttaagat cctatcaaca tagaatgatc ctaaaaatac aagtattgcc aatgttaagt
     1321 taagcccaat caaaaagtcg attaaatagg tgggcaatgg aattatgagc atggcgata
//
LOCUS       DAZKFZ010000417         1372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000417 DAZKFZ010000000
VERSION     DAZKFZ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            26..310
                     /locus_tag="ACHOQW_004985"
     CDS             26..310
                     /locus_tag="ACHOQW_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9575860.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKVSTNMIFKECRQSAAMKRVLLVYRT"
     gene            487..735
                     /locus_tag="ACHOQW_004986"
     CDS             487..735
                     /locus_tag="ACHOQW_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575861.1"
                     /translation="MHELFVLVLSTCASLSNMSGCSVEVVNVNTTKEPVNVFYSRKDC
                     EESMKNIMLNHALYHEVSGREPYMAKCEQVFVSKSLMK"
     gene            779..1060
                     /locus_tag="ACHOQW_004987"
     CDS             779..1060
                     /locus_tag="ACHOQW_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575862.1"
                     /translation="MEDYLVFGLGYEGDIKNDEAGLDKISVVTKSVVRSTNSNEPVVY
                     QVTEFKEFNVVRHQAFDSEYYNIAFDVLPSRVRIDAAIRKYHPKKSSSA"
     gene            1151..1252
                     /locus_tag="ACHOQW_004988"
     CDS             1151..1252
                     /locus_tag="ACHOQW_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575863.1"
                     /translation="MRTYNPNSLLPSLMQKCTCGSLHPTFDLCGGEA"
     gene            1249..>1372
                     /locus_tag="ACHOQW_004989"
     CDS             1249..>1372
                     /locus_tag="ACHOQW_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575864.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLENGKCFRMVLKPWRE"
BASE COUNT          414 a          280 c          325 g          353 t
ORIGIN      
        1 agttcggact gaaaggagca agtgtatgac gggcaaagag gcaattattc attacctggg
       61 gacgcataat agcttctgtg cgccggacgt tgccgcgcta acaggcgcaa cagtaaccag
      121 cataaatcag gccgcagcta aaatggcacg ggcaggtctt ctggttatcg aaggtaaggt
      181 ctggcgaacg gtgtattacc ggtttgctac caaggaagaa cgggaaggaa aggtgagcac
      241 gaatatgatt tttaaggagt gtcgccagag tgccgcgatg aaacgggtat tgttggtata
      301 cagaacttag catcgaaggc ctattgtttt cgacaggagg ccatcgaaat gacactatgg
      361 taccgtcttt acccaccgcc ttctgctaag tttaggaata caagatgttg ctgccgttaa
      421 aaaatagtcg taatttctat tagaatttgg gcaatataac cagcacatag ataagggagt
      481 cgttttatgc acgaactatt tgtgctggtt ttgagcacct gtgctagcct cagcaatatg
      541 tcaggttgtt cagtggaagt agtgaatgtc aacaccacaa aagagccggt aaacgtcttt
      601 tatagcagga aagactgcga agaaagcatg aaaaacatta tgctaaatca tgctctatac
      661 catgaagtat caggcagaga gccatatatg gcaaagtgcg aacaagtatt tgtctcaaaa
      721 agtttaatga aataattggc caaaaacgac atataacaaa ctcttatagg agcttaacgt
      781 ggaagattac ttagtttttg gtttaggtta tgaaggcgat ataaagaacg atgaagcagg
      841 gcttgataag ataagcgtgg taacgaaatc ggtagtgcgt tctaccaatt ccaatgaacc
      901 agtggtttac caagtgactg agtttaaaga atttaatgtt gtgagacatc aagcgtttga
      961 tagtgaatac tacaacatag cattcgatgt cttaccatcc cgcgtccgta ttgatgctgc
     1021 aatccgtaaa tatcacccca agaaatcctc ctcagcttag taagctttga ttttccatta
     1081 tcaaccagca ataataatgt cctcggagcc tgaacaactc cggtgacttc tgcgctaaac
     1141 ggggacgttt atgcgcacat acaatccaaa ctctcttctc ccttcactga tgcagaaatg
     1201 cacctgtggt tctttgcatc caacgtttga cctctgcgga ggtgaagcgt gaacctccca
     1261 caagacggta tcaaattgca tcgcggtaac ttcactgcta tcggccagca gatccagcct
     1321 tatctggaga acggaaaatg ctttcgcatg gtgcttaaac cgtggcgtga ga
//
LOCUS       DAZKFZ010000418         1367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000418 DAZKFZ010000000
VERSION     DAZKFZ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..392)
                     /locus_tag="ACHOQW_004990"
     CDS             complement(<1..392)
                     /locus_tag="ACHOQW_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575865.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLS"
     gene            complement(645..1268)
                     /locus_tag="ACHOQW_004991"
     CDS             complement(645..1268)
                     /locus_tag="ACHOQW_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9575866.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(1265..>1367)
                     /locus_tag="ACHOQW_004992"
     CDS             complement(1265..>1367)
                     /locus_tag="ACHOQW_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575867.1"
                     /translation="AVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
BASE COUNT          399 a          347 c          270 g          351 t
ORIGIN      
        1 gacagtagca aaagaggtac ctgaatcttc tgtctcaata tagaaaactc aataattgcc
       61 ggcacaaaca ataattccac agcaaaataa aggcgaaata catttagctc ttgcatagaa
      121 tgttttcttt tcactgcgaa aaagaataca acaccaatac cccaaccgat aagaaatata
      181 gcaatgacga taactgcaaa aaataaactt ctggcaacat catcaacacc tgcacctaca
      241 atccaccatg ggaagccgta gtaaaaagaa gtaccccatc catagaaata agcactcccc
      301 catccaaggc atcccatgta ggcaataaaa agtgaagaac tcctgagcag cgcaccatcc
      361 ttcataacca ccccaataca agatgataac attggcttac aactcataac aaaagcaatt
      421 caatgccgtc aagaggttac aggctaaaaa aactctatta cattgcagtc agcatgttta
      481 ctacacaaat acaattcaga gcataaaaac tactcggcgg caggttattg agactcatca
      541 atgacatgta aaaaacgccc attattggtg tcaagtttcc ccaaagttat tcaaaaagtc
      601 aatattatgc cgttaatatg ttgccatccg tggcaatcat ggcgctaacg tgtgatcgca
      661 ttcaaaatgt tgtctgcgat tgactcttcc ttgtggcatt gcacaaccag agcgtcatac
      721 agcggcttaa cagtgcgtga ccaggtgggt tgggtaaggt ttgggattag catcgtcaca
      781 gcgcgatatg cggcgcttgc tggcattctt gaataaccga cgcctttaca tcttccgcac
      841 tctttctcag caactatccc ccactgctct gttttggcta tatcaaccgc acggcctgta
      901 ccgtggcaat ctctgcatct tgcgcccggc gtagcggcac tacggcaata atccgcataa
      961 gcgaatgttg cgagcacttg cagtaccttt gccttagtat ttccttcaag ctttgccacg
     1021 ccacggtatt tccccgatac cttgtgtgca aattgcatca gatagttgat agccttttgt
     1081 ttgtcgttct ggctgagttc gtgcttacca cagaatacag ccattccgaa tccggcttgt
     1141 gattgcgcca tccccatagc agccatcaca tcagtaccgg aaagagagtc agaagccgtg
     1201 gcccgtggtg agtcgctcat catcgggctt tttggcgaat gaaatttagc tacgctttcg
     1261 agtctcatgc gccttctccc tgtacctgaa tcaatgtgag gtttccgcag aacactgcgc
     1321 cagtatcgat atacatctgg ttggcaaatt tgagtggttt cactgct
//
LOCUS       DAZKFZ010000419         1358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000419 DAZKFZ010000000
VERSION     DAZKFZ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            64..1338
                     /locus_tag="ACHOQW_004993"
     CDS             64..1338
                     /locus_tag="ACHOQW_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP9575868.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
BASE COUNT          417 a          286 c          271 g          384 t
ORIGIN      
        1 cactcatcat cccttgaatg aacgtcattt gatgtattat tcttacactt ggattaattt
       61 aagatgatac ctatgaaaaa ctatcttttc cagacacccg aagatatttg tgtacagtta
      121 aaaaaaatga cacatcctgt cacaataaga acaacagata ttgctaattt ctggcactat
      181 cttgagtcag caacacttcc agtgatcaca aaaagcacca ctacagaaaa tcgggaggtt
      241 acatttctgt ggcgctcaga gaaagcactg caaggcgtat atcttcgcct gaatcgtgtt
      301 acagataaaa aagacgtcaa aaaaggacta atgactcata tcccttcgac agatatctgg
      361 atgctgacac tggtgttacc agcttcatat cggggctcat actcatttat agaaattccc
      421 acagatatga cacaaaaaga catatttcaa ctaggaagtc gcttctctcc attacccggt
      481 aaatctgatc catttaacaa aacagcagaa ataaatatac gaggattcgg agaatcagtc
      541 ctttctcttg atatggctcc tgaacaaaag gaatgggatg atacttccca taaatgtaca
      601 ggtattcttt caacattaca ttcctttgtt gcaggatatc aacgccggat tcgtttatat
      661 tttcccctga atccaacatc agtacctctt ggattacttg tgttacctga tgctgaaaca
      721 tggtttgacc ggatagatat tacccgggca ttagatatgg ccatcaccac tggtcgtatc
      781 acgccaatgg caattatggg gatagacaat attaatgaat ctgatcgtat gaatatactg
      841 ggaggcaata aagaactcat ctttgatata gcggaaaatc tgatacccca gttatacaga
      901 gactatccgg atatcgtatg ggctggtcgc tctaatactg tactggccgg gcagagcctc
      961 ggtggagtga cagcactgat ggcagctata tatgcgtcga caacatttgg tacaatcatt
     1021 agtcactcac cttcaatgtg gtggaaccct gaccagggca gcccgatctt gtttactgag
     1081 aataatacct cctgggtaag tgagcagata ctttcagcgc ctccgaaaga tgtaaatatc
     1141 cgacttggag tcggttcttt agaaggtaca accgtctcac atgttcagcg attgcatcat
     1201 tcgttaatcg cagctggttt ggaaagtaac ctcactgtct ataccggtgg tcacgattat
     1261 gcttggtggc gcggagcaat tattgatgaa ttagcaaatt ataattgcag gaaggtatca
     1321 aataataact ctgtgtaaaa tatcgtttta tcgtccga
//
LOCUS       DAZKFZ010000420         1341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000420 DAZKFZ010000000
VERSION     DAZKFZ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            139..>1341
                     /locus_tag="ACHOQW_004994"
     CDS             139..>1341
                     /locus_tag="ACHOQW_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFP9575869.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIY"
BASE COUNT          374 a          323 c          316 g          328 t
ORIGIN      
        1 tactactaca ttaccgcatg attgaatgat tctcaattac atatccgatg gtaaaataac
       61 aaacggatag tgcttagtga gcaacaagaa acaattttca ctcacgacaa tccgtgagtt
      121 tttgggataa cagactaaat ggctaagttc acaccttcat tctcaggaat caaaggtcgg
      181 gcgctctttt cactgctctt tgcagcacca atgattcatg caaccgacac tgcaacgacc
      241 aaagatggcg aaacaatcac tgttacagcg gatgcaaata ccgcaactga ggcgaccgat
      301 ggttatcaac ctctgagcac ttccacggcg acattaaccg atatgccgat gctggatatc
      361 ccgcaggtgg tcaatacggt tagcgatcag gttctggaaa atcagaatgc aacgacgctg
      421 gatgaagcgc tttataacgt cagtaacgtg gtacagacca atacattagg cggaactcag
      481 gacgcctttg tacgtcgtgg ttttggtgct aaccgggatg gctccatcat gaccaacggc
      541 ctgcgaactg tacttcctcg cagtttcaac gccgccacag aacgtgtgga agtgctaaaa
      601 ggtccggcct ccacgctgta tggcattctc gatcctggtg gattgattaa cgtcgtgacc
      661 aagcgcccgg aaaaaacatt ccatggttct gtctcagcca cgtcctccag ttttggcgga
      721 ggcactgggc aacttgatat cacaggtccc attgaaggca ctcagctggc ataccgcctg
      781 acgggggaag tgcaggatga agattactgg cgaaacttcg gtaaagagcg cagtacattt
      841 attgccccgt cactcacctg gtttggtgat aatgcaacag taaccatgct ctattcccat
      901 cgggactata aaactccgtt cgatcgtgga acgattttcg accttacgac gaaacagccc
      961 gtaaacgttg atcgaaaaat acgttttgac gaaccgttta atattacaga tggtcagtcc
     1021 gatctggcgc aactcaacgc agaatatcat ctcaatagcc agtggacagc gcgctttgat
     1081 tacagctaca gccaggataa atacagcgac aatcaggctc gcgttaccgc gtatgatgca
     1141 acgacaggaa cgctgacacg gcgtgttgat gcaactcagg gatctaccca gcgtatgcat
     1201 tctactcgtg cggatctgca agggaatgtt gatattgctg ggttctataa tgagattctg
     1261 ggtggggtgt catatgaata ttatgatctt ctgcgcacag atatgattcg ctgtaaaaac
     1321 gctaaagatt tcaatatcta c
//
LOCUS       DAZKFZ010000421         1321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000421 DAZKFZ010000000
VERSION     DAZKFZ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            488..679
                     /locus_tag="ACHOQW_004995"
     CDS             488..679
                     /locus_tag="ACHOQW_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575870.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            complement(749..889)
                     /gene="ykgO"
                     /locus_tag="ACHOQW_004996"
     CDS             complement(749..889)
                     /gene="ykgO"
                     /locus_tag="ACHOQW_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP9575871.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(889..1152)
                     /gene="ykgM"
                     /locus_tag="ACHOQW_004997"
     CDS             complement(889..1152)
                     /gene="ykgM"
                     /locus_tag="ACHOQW_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9575872.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          389 a          272 c          291 g          369 t
ORIGIN      
        1 tgcattttat attagccagg aactttgccc tgaggtgtta ggaatagtct taatgtgttg
       61 tgtaatggac tagtaaaaaa acaagtctaa atgataagaa atagaatatg aaactatcat
      121 gagtcataat taaagatgat ttgccaatca gtaaggtgtt ttaatggatt aacatgccgc
      181 acaaaatggt tatgggtgtt catcccaata agtgatgaaa aattaaacag gtgtaagtac
      241 gtcatctgtc cggtaagccc cctaagagcc agattaagtg aagttaaacg aagactgcca
      301 gttgtcaccg gaacgatggc ctctttgcca gggcactgca ttcagtcatc tgtgcgcgtt
      361 actctaactg gcactcgaac gtcggcgacg gggttaccgg tggatcaggc cgttcctgtg
      421 gcgtaaacgt ttttatgtca gtaccaacag atttaccaca tttatcatct taacagtttg
      481 agtgtaaatg caggctgcgt cgcaaaggga ggaaactgag tggcgtgttc agtctaaaag
      541 agggttgatg ccagcataca ggggcgaagc tggtcaacaa gtcaacatta atataatgga
      601 atattcggag agaaacgtca ggcagttgac cttaaatgaa caggaagata catccccagg
      661 aaaattaatg ctggtgtaat aaatacacca acattaatcc ggtctgatta ttaatcagac
      721 aaatatcgtt aattcgtcga attttaaatc aacgtttttt cttacgaccc tgaacggcct
      781 taaagcgtgg attagattta caaatcacat atagccgtcc ttttcgcttc acaatctgac
      841 agtctggatg gcgttctttt gcggtacgca gagagttaag gactttcatc acgccccctt
      901 tttcgtgcta acaaaacgac caaaacgttg ggtgaatcgt gcaacatttc cttctgatgc
      961 cactgttctc agcttccctg tatagaacgg gtgcgatttc gaagagacat caattgttac
     1021 gtacggatac gttacgccat ccagctcaat ctcacggtct gttttgatag tcgagccgat
     1081 tttaaagtac tcatcaacac tggtgtcgtg gaacaccaca gtacgatact caggatggat
     1141 attgggcttc atcattttta cctgttatgt tataacataa ccacaaagat acatgctcat
     1201 ccggatcatt tcaacaccca atccctgtga ccattagtgg tatgtggaat ttcctcaacg
     1261 gggaagtctg gcattgttaa cagccattgg cgacgctccc ataaacgcct ggatgagtga
     1321 t
//
LOCUS       DAZKFZ010000422         1314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000422 DAZKFZ010000000
VERSION     DAZKFZ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1314
                     /gene="rhsB"
                     /locus_tag="ACHOQW_004998"
     CDS             <1..>1314
                     /gene="rhsB"
                     /locus_tag="ACHOQW_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFP9575873.1"
                     /translation="ARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVTSGHPVN
                     PLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRD
                     NTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEEL
                     RLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHR
                     EAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTE
                     YGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSFTYDD
                     KYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREV
                     LHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEY"
BASE COUNT          256 a          399 c          443 g          216 t
ORIGIN      
        1 gcgcgtcagg gcgacatgac gcagtatggc ggtagcattg ttcagggttc agccggggtg
       61 cgcattggtg cccccaccgg cgtggcctgt tcggtgtgcc ccggcggagt gacgtccggc
      121 catccggtca atcccctgct cggtgcaaag gtccttcccg gtgaaaccga catcgccctg
      181 cccggcccgc tgccgttcat cctctcccgc acctacagca gttaccggac aaaaacgccc
      241 gcgccggtgg ggagcctcgg ccccggctgg aaaatgcctg cggatatccg cttacagctg
      301 cgcgataaca cactgatact cagtgataac ggcggcagaa gcctgtattt tgagcacctg
      361 tttcccggtg aggacggtta cagccgcagc gagtcactct ggctggtgcg cggcggcgtg
      421 gcgaaactgg atgaaggtca ccggctggcc gcactctggc aggcgctgcc ggaagaactc
      481 cgcttaagtc cgcatcgtta tctggcgaca aacagtccgc aggggccgtg gtggctgctc
      541 ggctggtgtg agcgggtgcc ggaagcggat gaggtgctgc ctgcgccgct gccgccgtac
      601 cgggtactga ccgggctggt ggaccgcttc gggcgcacac agacgttcca ccgcgaagcc
      661 gccggtgaat tcagcggcga aatcaccggc gtgacggatg gtgccgggcg tcacttccgg
      721 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
      781 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      841 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      901 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggtggtgtat
      961 gaccgtagtg gcaaacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
     1021 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
     1081 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaagaccgc
     1141 atcaccatca ccgacagcct gaaccgccgt gaagtcctgc acacgcaggg tgaaggcggg
     1201 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgac
     1261 gcggtgggca ggctcagggc acagacggat gccgcaggca ggacaacaga atac
//
LOCUS       DAZKFZ010000423         1312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000423 DAZKFZ010000000
VERSION     DAZKFZ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(39..227)
                     /locus_tag="ACHOQW_004999"
                     /pseudo
     CDS             complement(39..227)
                     /locus_tag="ACHOQW_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          385 a          289 c          237 g          401 t
ORIGIN      
        1 aaccagcaaa cgggcattgt gttctgaaat ccaggctatt gattcaacgt cagcgacaga
       61 tctgcgctgt aaaagaactt gtaaaacaac gtaatagaat tatccggaat ggtggcgact
      121 atgcactaga acaccacaat ttcgctctct cggtaaggca ctgtcacagc attcaacaga
      181 atgtgacttg ccagatttat tagcaccacc agatgtttaa ccgggtaacc acgacccagt
      241 cggggatata ttccatcttc tcgctgacat catcaagaat aaaaaggttt attacactca
      301 gagcaacgtt cttcagcagg cgggataatt cttcaacatt tagaagatgt gttattaaag
      361 gcatacaaac tggacgtatt gtattttctt ttggtggttg atctaaggta gcagtttatc
      421 ctgatgcgct gagatttctg taatatctac gtcaacattc tcctcgaata gaaaatgcag
      481 ccccggcaag taattcacat tttttaacaa aacgttgtta gcgtgccatt ttcagaatct
      541 ctccaagaaa gcgaattctt ttctcaaatt ctgcattctc agacacagct ttctactcca
      601 tggtaatggc ccaaatacga agttgctcag gatcattagt ggtgcttaga agttaaataa
      661 ccatgcggag taccactcgt catcacgtat ttatatatca tgaagctatt gttgtgtttt
      721 gtacatcagt agatatattc tgatatactc cttttgctag acataacctt tcacctgctt
      781 gcaaagcttc tgtgttctga cattgccaaa ttgttgcaat tctgtatcca gccttctttc
      841 agtcatagct tcgggccgcg ataagactca ctgatctgac cctgattcct cttgcagact
      901 ttatagacca attaaaatgc agtttctgca ggtcaacgtc tgaccatcat tgtcatcact
      961 ctggccatta gagtaacctt ctgcattcat ccttttgtaa aaagtttata ttagtatcag
     1021 caattaaccg gacctgatac tgatatgagt cttaccgcat atacggtcaa tttcagcaat
     1081 taattacatt atccacgcca aagtatttgt catcacaatg atggtacctt ctttcagaca
     1141 ccattttttc aactccgttt tccacggacc gcactcttat gtcaagagtg cggtccgtgg
     1201 atacaaccag agaccgactg acacgagtca gaggaaacga cggatatgtt cagtcgtaaa
     1261 atatctatca aaaaacatga ttaaggtcaa aaatgtttga tatttacaat tt
//
LOCUS       DAZKFZ010000424         1303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000424 DAZKFZ010000000
VERSION     DAZKFZ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1303
                     /locus_tag="ACHOQW_005000"
     CDS             <1..>1303
                     /locus_tag="ACHOQW_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFP9575874.1"
                     /translation="REDYGRETQREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSC
                     GYLKAQSAGGHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETER
                     FRWDSRDQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYR
                     DDELYSVRHLVFNGFELISQQCSRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSE
                     GKTVWRPGQTSLWGLALSLPADTDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRF
                     RYYEPETGMYLVSDPLGLQGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRR
                     VSGHTGFLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLR
                     KNATLYEVYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEI"
BASE COUNT          331 a          306 c          420 g          246 t
ORIGIN      
        1 cgggaagatt acggacggga aacacagcgg gagtaccggc ttgaccggaa cggccaggtc
       61 acggcggtga cagcctcagg caccgggctg ggctatggcg aaggcgatga gtcctatggc
      121 tatgacagct gtggctacct gaaggcgcag tctgcgggcg ggcaccggat aagtgaagag
      181 actgaccagt atgccggagg ccaccggctg aaacaggccg gaaacacgca gtatgactat
      241 gacgccgcag gccggatggt cagccgcacc agacaccgtg acggctatcg cccggaaaca
      301 gagcggttcc ggtgggacag ccgggaccag ctgaccgggt attgcagcgc acagggtgag
      361 cagtgggaat accgccacga cgccagcggc agacgaacag agaaacgctg cgaccggaag
      421 aaaatccgct tcacgtacct gtgggacggc gacagtattg cggaaatccg ggaataccgc
      481 gatgatgaac tgtacagcgt acggcacctg gtgtttaacg gctttgagct gataagccag
      541 cagtgcagcc gggtacggca gccgcacccg tccgtggccc cgcagtgggt gacacgaacg
      601 aatcatgcgg tgaacgacct gacgggccgt ccgctgatgc tctttaacag tgaaggtaaa
      661 accgtctggc ggccgggaca gaccagcctg tgggggctgg cactcagcct gcccgcagac
      721 acagactacc cggacccgcg cggggagcgg gacgcggaag ccgaccccgg cctgctgtat
      781 gcaggacagt ggcaggatgc agaatcgggg ttgtgctata accggttccg atattacgag
      841 ccggaaaccg ggatgtacct ggtgagtgat ccactggggt tgcagggagg ggagcagact
      901 taccggtatg tgccgaatcc ttgtgggtgg gttgatccgc tgggattggc tgcaagttct
      961 aaaatcagca gtttgatgga ctatattggc gatggtcgtc gtgttagtgg gcatacgggt
     1021 ttcctggatg gggttcgttt atcacgtagt caaataaaca atattgctaa agaaatggag
     1081 aagctaggaa ttaaagtaat aaggaaagca gataaatatt tgccaccaaa tgctagggca
     1141 gcttttgatt atggtcttcg caatatttat cttaggaaaa atgctacctt atatgaggtg
     1201 tatcatgaag tgattcatgc taagcaattt gcgaaaattg gacgagaagc atacgaagca
     1261 ctaggacgtt tatctaggga ggaacacgtt ctaaatgaaa tat
//
LOCUS       DAZKFZ010000425         1296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000425 DAZKFZ010000000
VERSION     DAZKFZ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1296
                     /locus_tag="ACHOQW_005001"
     rRNA            <1..>1296
                     /locus_tag="ACHOQW_005001"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          350 a          286 c          408 g          252 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      481 tgcaccgaag ctgcggcagc gacactgtgt gttgttgggt aggggagcgt tctgtaagcc
      541 tgtgaaggtg tgctgtgagg catgctggag gtatcagaag tgcgaatgct gacataagta
      601 acgataaagc gggtgaaaag cccgctcgcc ggaagaccaa gggttcctgt ccaacgttaa
      661 tcggggcagg gtgagtcgac ccctaaggcg aggccgaaag gcgtagtcga tgggaaacag
      721 gttaatattc ctgtacttgg tgttactgcg aaggggggac ggagaaggct atgttggccg
      781 ggcgacggtt gtcccggttt aagcgtgtag gctggttttc caggcaaatc cggaaaatca
      841 aggctgaggc gtgatgacga ggcactacgg tgctgaagca acaaatgccc tgcttccagg
      901 aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg
      961 tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt
     1021 aacttcggga gaaggcacgc tgatatgtag gtgaagtccc tcgcggatgg agctgaaatc
     1081 agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga
     1141 aagtggacgt atacggtgtg acgcctgccc ggtgccggaa ggttaattga tggggtcagc
     1201 gcaagcgaag ctcttgatcg aagccccggt aaacggcggc cgtaactata acggtcctaa
     1261 ggtagcgaaa ttccttgtcg ggtaagttcc gacctg
//
LOCUS       DAZKFZ010000426         1284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000426 DAZKFZ010000000
VERSION     DAZKFZ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            65..169
                     /locus_tag="ACHOQW_005002"
                     /pseudo
     CDS             65..169
                     /locus_tag="ACHOQW_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(289..>1284)
                     /locus_tag="ACHOQW_005003"
     CDS             complement(289..>1284)
                     /locus_tag="ACHOQW_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M85 family metallopeptidase"
                     /protein_id="HFP9575875.1"
                     /translation="NMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYVLDIGKRIP
                     LSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDAVSYGIHNE
                     KVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEAGENDNEEP
                     YVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEILARRVAQEL
                     GWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISDRYEASPDF
                     TEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVDKHNQQ"
BASE COUNT          338 a          286 c          270 g          390 t
ORIGIN      
        1 acggtacttt tctgtgcaca acgtactgca gtttctttac gatttatagc tgtactgttg
       61 aattatgtac aatttgaatc catgcatgac gtgtgatgcc tattgtgcat ttttccgagc
      121 ctctttttac tgggccgaag ccgatgatgc tgatagtctc gttccggcca tgttcactta
      181 ctacagtaaa attaggggaa ttgcctttac atacaatgtt gcacgattac ccatcatcaa
      241 ctccgttaaa atgtatgaat agtaacctta tgtcactgca aagacgaatc attgctgatt
      301 gtgtttgtcc acatccccaa atgtaaaaat ataaggggcg ccatgataca gttggatctg
      361 atttgcatcc tgtaaattat cgttgatagc caatccagga aaatcatgtt gctggataaa
      421 tccgtatcct atgttagaca cagcggaata ctctgtgaaa tcaggactcg cctcatatcg
      481 gtcactgatc gtacccagcc tttcgaagaa agccctctca ttctcttcat gcctcatggc
      541 agcctgtcga agggcattca ggttacgtag cctaagatga gcttcacgtt ctggctctgc
      601 atatcctttg aagtcgggaa cactccatcc cagttcttga gcgacacgac gtgcgagaat
      661 ctcggtgggt cctaactcta tattactatc tccagatgga tcgctagatc cagtaacatg
      721 atgaataatc tcgtgaatca gtccttcctg ccacgatggc atctcataag agtcagtatc
      781 tggcgcaaca ctaaaactga cataaggctc ttcgttatcg ttttctcctg cttcgcaaat
      841 ggggaaaatt ggctcttgcc cgacatcata ttcatataat tcgttacagg ttagtgattg
      901 aatatcatca attttgacag aagattcttc gttaagctcg tattcgtttc tgtatttaat
      961 gcaaccaatg tgtaccttct cattatgaat gccatagctt acggcatcac gaaatgtctg
     1021 tgatcggctc aaagcatcaa gtacagtgtt gccgatcata tcgactgtat gctgatcgat
     1081 aagcctgcta cggctgtcat ggacggcgcg tattacactt tcgtatacgt tgcttaaatc
     1141 tgctgcggaa agtggtattc gcttacctat atccaaaacg taatccgcat aggcattttc
     1201 agcacgatta ggagcaatgg gagcagagta tcctgccggg gcggaaaagt tgaagttgga
     1261 ctgtaatgag ggaattttca tgtt
//
LOCUS       DAZKFZ010000427         1283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000427 DAZKFZ010000000
VERSION     DAZKFZ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            159..761
                     /gene="ais"
                     /locus_tag="ACHOQW_005004"
     CDS             159..761
                     /gene="ais"
                     /locus_tag="ACHOQW_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP9575876.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(800..1225)
                     /gene="nudI"
                     /locus_tag="ACHOQW_005005"
     CDS             complement(800..1225)
                     /gene="nudI"
                     /locus_tag="ACHOQW_005005"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9575877.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT          347 a          282 c          275 g          379 t
ORIGIN      
        1 tattttttct tatttaggct ttgcatttgg caaaattttg aggcattttg ccagcatcgt
       61 aggattttta atattacacc aactgctaat tatcgccaga aatgtcgctc aatttcacgg
      121 taattgtctg gttgcgcttg tctataggtg gagtttacgt gttagctttt tgccgctcat
      181 cattgaagtc aaaaaaatat ttcatcattt tactggcgct cgctgcaatt gccggactgg
      241 gtactcatgc cgcctggagt agcaatggtt tgccacgtat cgacaataaa acgctgggca
      301 gactggcaca gcagcacccg gttgtcgttt tgtttcgcca tgctgaacgt tgcgatcgtt
      361 caaccaatca atgcttgtca gataaaacag gtattacggt taaaggtacc caggatgccc
      421 gtgaactggg caacgctttt agtgctgata tccctgattt cgatctttat tccagtaata
      481 ccgtccggac cattcagtcc gcgacctggt tttcagcggg taaaaaattg acggtagata
      541 aacgacttct tcagtgcggt aatgagattt atagtgcgat taaggactta caaagcaaag
      601 cgcctgataa aaatatcgtt attttcaccc ataatcattg cctgacatat attgctaaag
      661 ataagcgtga tgcgacattt aaacctgatt atctggatgg tttagtcatg catgtggaaa
      721 aaggcaaagt ttatctggat ggggaattcg ttaatcacta aattttcaat ctgacagcca
      781 gtaatggctg tcatcattgt tacagaagac ctttcaaacg taacgttttt cgggtggcga
      841 cattcaaatc atagtgcgcc agatcttcag gttttaccca cgcgtagtcc tgaaactctt
      901 cgtttatttt cacttctcgg ttggcagaaa cgcaatcaaa aatcaggtaa atcatataaa
      961 tttcttcctt gcgaccatct gcatacgtct tggtgcgaat atcatcgctg aaggtccagg
     1021 gcgtgatttc tgtcaaaagc agctgttctc ccagttcttc gcgaatttcg cggcgtagag
     1081 cctcttcaat tcgttcgccg ggctccacgc cgccacccga aagcgcccat tgaccgggga
     1141 aaacgccgcg atcgtcggcc attttacaca gcaaataagc accatcattt tgaatcaaag
     1201 ggcatacaat agtccgttgt cgcacagggg tattccttat aaataaaaag aaaccatgag
     1261 aatacggtaa ctcagaaata tgt
//
LOCUS       DAZKFZ010000428         1279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000428 DAZKFZ010000000
VERSION     DAZKFZ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            45..166
                     /locus_tag="ACHOQW_005006"
                     /pseudo
     CDS             45..166
                     /locus_tag="ACHOQW_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            228..926
                     /gene="nleD"
                     /locus_tag="ACHOQW_005007"
     CDS             228..926
                     /gene="nleD"
                     /locus_tag="ACHOQW_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFP9575878.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(1023..1115)
                     /locus_tag="ACHOQW_005008"
                     /pseudo
     CDS             complement(1023..1115)
                     /locus_tag="ACHOQW_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          355 a          255 c          299 g          370 t
ORIGIN      
        1 ttttatccag tgtagtggat ttgttgcatg gatggagttg gtaacaaatt gtgtggttgg
       61 caattcttgg cggatcacgg aaatgggcaa tgtcgctgag gactggcaaa tggctatgat
      121 ccgctttatt atcgggtttg gtgaccgcct tgacggtcac ttttaaaaaa atcattttca
      181 cagagtggta aacaggctgc taataagtag cattctcagg agtcctgatg cgccctacgt
      241 ccctcaactt ggtattacat cagtcatcaa ggtcgagctc aatgtcagat acagatatcg
      301 agtctcttgt aaaagcatcg agcgttcaat ggataaaaaa taatccgcaa cttcgtttcc
      361 aggggactga tcataatata tatcagcaga ttgaagcagc actcgataag attggctcta
      421 cagagacagg gcgtgtactc ctgaatgcta ttgaatcaat atcccgactt aaatcagaaa
      481 cagtggtaat acacctcaac tcttccagac taggagttat ggcacataga gatatagatg
      541 ctgagaacca tcgggggact ggttccgatt ttcactgtaa tctgaatgca gttgaatatc
      601 cctgtgggga ggggattagc gtggtggact ttcatgcgac tattgttttt catgagttgc
      661 tccatgtttt ccacaattta aatggggagc gtttgaaagt tgagagttcc cgagcagaat
      721 cacaaaaata ctctccactt ttactcgaag aagccaggac tgttgggttg ggggcttttt
      781 cagaggaggt gctttcagaa aataaattcc acgaagagat tgggatgccc cgtagaacct
      841 cctacccgca cgactcagct cttattcatg atgacaatac agtgagtctg ggattccaac
      901 aggtaagact gcatccattg ctttagctgt gtttcttgtg ctgaatatat attggtgggg
      961 caaacggagg cacacaaaac tttgcactgg attgcaaggc tttgtgctat tccgatagtg
     1021 gttcaggtgg agtaccccac cttttcatca agccagccca cccaccactg catcatttct
     1081 ctgcgcttat agagatactg agcatggttg taaattcagc aaacgacgta atgtgtttga
     1141 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
     1201 aataccatct aagtagttga ttcatagtga ctggatatgt tgtgttttgt agcattatgc
     1261 agtctatttt ttagactaa
//
LOCUS       DAZKFZ010000429         1271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000429 DAZKFZ010000000
VERSION     DAZKFZ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..225
                     /locus_tag="ACHOQW_005009"
     CDS             <1..225
                     /locus_tag="ACHOQW_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9575879.1"
                     /translation="LIEITESERQLLINQANEYMNSKQWPGKAAIGRLKGEELAQYNL
                     WLDYLDALELVDTASAPDIEWPTPPAVQAR"
     gene            complement(197..799)
                     /locus_tag="ACHOQW_005010"
     CDS             complement(197..799)
                     /locus_tag="ACHOQW_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9575880.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAETQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETNRLNTVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(799..>1271)
                     /locus_tag="ACHOQW_005011"
     CDS             complement(799..>1271)
                     /locus_tag="ACHOQW_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575881.1"
                     /translation="LPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKVYPTNKLP
                     DLRGEFIRGWDDGRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTIL
                     KQITPLSPAINFDNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
BASE COUNT          315 a          311 c          303 g          342 t
ORIGIN      
        1 cttattgaaa ttactgaatc agaaagacag ctactaatta accaggccaa cgaatacatg
       61 aacagtaagc aatggcccgg taaagccgct attggtcgtc tgaaaggtga ggaactggca
      121 caatataatt tgtggctgga ttatctggac gcactggagc tggtcgatac cgccagtgcg
      181 ccagatattg aatggcctac gcctccggca gttcaggcca gatgacatcc ggcgcggtgc
      241 tggtatctgt tgccgtcacc gcgtcaatgt aatccagcac cgtgttaagt cggttggttt
      301 ctgcctgcgt cagcttccgt ccggcctgta atttcagctg aatcagacta atggaagcca
      361 ttgcagtatc aatcagcgac tggcgttgtg tttctgccgc ttctactgcg gcgctatgct
      421 gtgcttcggt atccgtcacc catttctcac catcccattt atcgtatggc gttaacgggg
      481 cgatagtggt tgtattatca gggtaatcac ccggagcggt gatttctttt gattctcctg
      541 tttcggtgct aaagacgatt tcaccgcgat ggtctggcac atattcccat gagttaaaat
      601 ctgcagaacg gcagattgca taaccagctt tgtatgcgcc tggggcatct aaacaggaat
      661 atgccggaat gccgacacca atagcaagat attcagttga agctgaaata tattcccgac
      721 tcatgacatc aaagttataa acagtaattt ctcctgccat agtggcaatt aattcactgt
      781 ttaattctgc gttattcatt atgcagccct cacaatatag ttaaaagcga catttcgtgg
      841 gcgtgtttca ttcgcacctg caggtttagt cgttgccgca acaacaccac cctctgtaat
      901 agccgggttg ggaattgggt aattatcgaa gttaatggcc ggagacaggg gcgtgatttg
      961 ttttaaaatc gtggtgcctg taaaactttc cagagtctgg tcgttagttc gtgaagtcca
     1021 gattccccaa tagtggttat gactgacaat cgcaccgtcc tgaagcgtca ataatcccct
     1081 tccgttatcc actccacgtc cgtcatccca gccacgaata aactcaccac gtaaatcagg
     1141 caatttattt gtcgggtaaa cctttgccag ttccgggtat tcttcagcag aaaaagccgc
     1201 cccgttgcat ttcagccagc ctgtcggcgg agtggctgaa ggccacggaa caggcacacc
     1261 aacgggtaat g
//
LOCUS       DAZKFZ010000430         1261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000430 DAZKFZ010000000
VERSION     DAZKFZ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..96
                     /locus_tag="ACHOQW_005012"
     CDS             <1..96
                     /locus_tag="ACHOQW_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9575882.1"
                     /translation="EMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(287..814)
                     /gene="sodC"
                     /locus_tag="ACHOQW_005013"
     CDS             complement(287..814)
                     /gene="sodC"
                     /locus_tag="ACHOQW_005013"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9575883.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            948..>1261
                     /locus_tag="ACHOQW_005014"
     CDS             948..>1261
                     /locus_tag="ACHOQW_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575884.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TIT"
BASE COUNT          311 a          294 c          369 g          287 t
ORIGIN      
        1 gaaatgctgg ttggctcccg gcgaatctcc caggacatca gtacccgtga tgaaggcggt
       61 gacgggaagg tggtggttat cgggcggcag gcataaaagc gaaaaaatcc cgcagtgctc
      121 acggacagga actgcgggag cgttacgaag attgagtgta aggaattatt cttatgtcac
      181 gacaaaaaca ttaactcaga gagggaggat gtgccgttct tttcagggag aaaggattta
      241 tcgtcctgag gaataaaggt aaggggcccg ccccttacct gactgattat tgaatgatgc
      301 cgcaggccat tctcgcacca ccaccgccca ggggctccgg atggtcatga tggttatcac
      361 cgccagcatg aagcatgaga gaacgccctt taatctcttt taatgagttc agtctcgggg
      421 ccaggaccgg gtagttcgct tttccgtcat gcgtcacgaa cagcgcaggg aggtcgccca
      481 ggtgtccatc cggagaccag gggccaagat gtttgccggt gtttttcggg tcaaagtgac
      541 cgccagccga taatgctgcg accggttttc cgtctttcag tgccggggcg caatttcctt
      601 tttcgtgcac atgaaaacca tgaatgcctt cagacagaga gtgaagggct ggtgtgaaca
      661 gcagaccgta gggggtctcc tgaatggtta tttttccaat gctgacttct tttccgtcag
      721 cactgacaag gttcattggg acttcctgtt ctgctgcgta tccgcatgat gctgctgtca
      781 gcatggcaat ggcagcaatg attttacatt tcataaaacc ctcattaatt ccgttaacag
      841 actgagcttg ctggttacag ggtaacaaac agcgttctga tgatatcgcg caatagctgt
      901 gcaatatcct atcactgcga ttaataatac caattgagag gaacattatg ggtaaaggtg
      961 gcggcagggc gcacacgccg gttgaggcaa aggacaatct taagtccacg cagatgatga
     1021 gcgtgattga tgccattggt gaagggccga ttgaaggtcc ggtgaagggg ctgcagagta
     1081 tcctggtgaa caaaaccccg ctgacggaca cggacggtaa tcctgtgata catggtgtga
     1141 cagcggtctg gcgcgccggg gagcaggagc agacaccacc tgaaggcttt gagtcttccg
     1201 gagctgaaac cgcactgggc gtggaagtga cgaaggcaaa gccggtgacg cgcaccatta
     1261 c
//
LOCUS       DAZKFZ010000431         1259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000431 DAZKFZ010000000
VERSION     DAZKFZ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            50..574
                     /gene="lpfA1"
                     /locus_tag="ACHOQW_005015"
     CDS             50..574
                     /gene="lpfA1"
                     /locus_tag="ACHOQW_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFP9575885.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            639..>1259
                     /gene="lpfB"
                     /locus_tag="ACHOQW_005016"
     CDS             639..>1259
                     /gene="lpfB"
                     /locus_tag="ACHOQW_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP9575886.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGALNITLPAGVGSVSK"
BASE COUNT          349 a          262 c          295 g          353 t
ORIGIN      
        1 aatacatcaa gattttcttt ttaatgtaat tttttaaatg gagtttttca tgaaaaaggt
       61 cgtttttgcc ttaaccgcac tggcgttaac ttctggcact gtattcgcag cagagtctgg
      121 tgacggcacg gttaaattta caggcgagat cgtggattca ccttgcgtac tgtccgttga
      181 ctctcagaac caggaagttg tgttgggtca ggtacaaaaa tctgtatttg ctgcggttgg
      241 tgacaaatcc ccggcaaaac ctttcgaaat caaactggaa gattgcgata ccaccacgat
      301 gaaaaaagcc aatgtgagct ttagtggtgt tggtgatgca gataaatctg atctgatttc
      361 tgttagcact gaagcgggcg cggccaaagg tgttggtatc ggtatttatg ataacagcaa
      421 taccctggtc gctcttaacg gtggtaaagc aagtgttgat ctgagtaaag gccagacggt
      481 tctctacttt accgctaact acgtttctac tctggcaacg gtaacgaccg gttacggcaa
      541 tgcgcaggtc gatttcaacc tgtcttacga gtaatcgtcc gttttacaaa tggcggcgta
      601 attgccgcca ttagtcgtca aagttcatgg atcgaatgat gaaaagtaaa tttgttgcgt
      661 tggctctctc tctgttcctt tcacagtcag ttctggcggg tggcgtcgga cttagctcta
      721 ctcgcgtaat ttatgatggc agtaagaaag aagcctcgct gaccgtacag aataaaagta
      781 agacggaaga gtttcttatc caatcctggg tggatgatgc cgcgggcagc aaaaaaacac
      841 cgtttattat tacgccccca ctattcaagc ttgatccaga aaagaacaat attttgcgta
      901 ttgtgaatat tacgcccggc ctgcctcagg atcgcgaatc ggtctactgg gttaatgtga
      961 aagccattcc atctaaaagt gacgacagcg aaaacaaaaa tgttttgcag attgccgttc
     1021 gcacccgaat caaacttttt tatcgcccgg caggtttaaa gggtgatgta aaaaccgcgc
     1081 caaatgaact gcgatttacg cgtaacggta atcagctccg cgttgataat ccgacagttt
     1141 ttaatattac ctttaatcaa ttttttgcga atgacaaaga aattgaaaaa gcaggcatgg
     1201 tacccgctaa aggggcatta aatatcacat tacccgccgg ggtaggtagc gtttctaaa
//
LOCUS       DAZKFZ010000432         1234 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000432 DAZKFZ010000000
VERSION     DAZKFZ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            344..538
                     /locus_tag="ACHOQW_005017"
     CDS             344..538
                     /locus_tag="ACHOQW_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575887.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            881..1009
                     /locus_tag="ACHOQW_005018"
     CDS             881..1009
                     /locus_tag="ACHOQW_005018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575888.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1017..>1234)
                     /locus_tag="ACHOQW_005019"
     CDS             complement(1017..>1234)
                     /locus_tag="ACHOQW_005019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575889.1"
                     /translation="RQNQRSITMRFSSVLKKFFITALFRPFPLCGIFCATPWGFNFAY
                     SLQFDIANPDPYAATGHPLAGHCECIV"
BASE COUNT          388 a          284 c          280 g          282 t
ORIGIN      
        1 cagaccacca ggctgatagc tcacagtttg ttctccgatt caataaaacg gataagaacc
       61 atcagcgcgg tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt
      121 atcaccagaa gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat
      181 tatcgaacag caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc
      241 tcctttagtt gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt
      301 ttcattagca cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat
      361 aaaaaggctg gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg
      421 tgccgcgctg ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa
      481 tggcaaaaaa atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca
      541 acaatctgca tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc
      601 tttatatcca ctgacgagtt tgccggtcat tctggtatca tgcagatatt tacggcgtac
      661 aagcacacta gcatgacatt gtaataaggc ctgagcctaa cgctttaatg tgtgtcatac
      721 actcagtggc gtaatcgttg acaatatggt gcatgtaatc gtaagcgtcc agcgaaatcc
      781 atctggtcat aatctgaagc atccgacaaa gtggtgtcca ccaaataagt gatctgcgcc
      841 caatatcctg agccggaact ttgacaaagg ccaacgaaaa atgggagcgg atgttacaga
      901 gttctcggta caggggaaaa gtggtacctg tcgccagttc tccgatctgt ttaaccggga
      961 aattatctcc tacagcttgt cagaaaggtc ggtgatggag catcgttaat acgatgctat
     1021 acgatgcatt cacagtgccc ggccagagga tgccccgtcg ctgcatatgg atcagggttg
     1081 gcaatatcga attgcaggct ataggcaaag ttaaagcccc atggagtagc acaaaatatg
     1141 ccgcacaaag gaaacggtct gaataacgca gtgatgaaga acttcttcag cacactgcta
     1201 aaacgcatag tgatcgagcg ctgattctgg cgaa
//
LOCUS       DAZKFZ010000433         1221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000433 DAZKFZ010000000
VERSION     DAZKFZ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            52..141
                     /locus_tag="ACHOQW_005020"
     CDS             52..141
                     /locus_tag="ACHOQW_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575890.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            161..>1221
                     /gene="nleL"
                     /locus_tag="ACHOQW_005021"
     CDS             161..>1221
                     /gene="nleL"
                     /locus_tag="ACHOQW_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFP9575891.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSV"
BASE COUNT          380 a          185 c          248 g          408 t
ORIGIN      
        1 gaatatttgt tagataaaaa agaggagata attcaatagg gagttaaatt aatgccgata
       61 aatctgacat cttatttgtg ggtacaggga cagaaagttg tcccggcagt tgtgttttct
      121 aattttactt tagtggacta agtaaaaagg agtgagataa atgctgccca ctacaaatat
      181 ctctgtaaat tctggagtaa tatcttttga aagtcctgta gattcaccat ctaacgagga
      241 tgttgaagtt gccctcgaaa agtggtgtgc tgagggagaa tttagcgaaa atcgtcatga
      301 ggttgcatca aaaatacttg atgttataag tactaatgga gagactttat caatcagtga
      361 gccaataaca acattaccag acttgcttcc aggttctctg aaagaactgg ttttgaatgg
      421 atgtacagag cttaaatcaa taaactgctt accccccaac ttatcttcat taagtatggt
      481 tggatgctca tcattagagg ttataaattg cagcatacct gaaaatgtca ttaatttatc
      541 tttatgccat tgtagttctt tgaaacatat agaaggttcc tttcctgagg cactcagaaa
      601 ttccgtatat ttaaatggct gtaattcatt aaatgaatcg caatgtcaat tccttgcata
      661 tgatgtcagt caaggccgtg cctgcctgag caaagctgag cttactgctg acttaatttg
      721 gttgtcagct aaccgaacgg gtgaagagtc tgctgaagaa ttgaattact ctggatgtga
      781 cttgtcaggt ctaagtcttg tagggctgaa tttatcatca gtaaattttt ctggagcagt
      841 gcttgatgat acagatctca ggatgagtga tttgtctcag gctgtattgg aaaactgttc
      901 ttttaaaaac tcgattttga atgaatgtaa tttttgttat gctaatttat ctaattgtat
      961 tattagggct ttgtttgaaa actctaattt cagcaattcc aatcttaaaa atgcatcatt
     1021 taaaggatct tcatatatac aatatcctcc aattttgaac gaggctgatt taacaggagc
     1081 tattataatt cctggaatgg ttttaagtgg tgctatctta ggtgatgtaa aggagctctt
     1141 tagtgaaaaa agtaatacca ttaatctagg agggtgttac atagatctat ctgacataca
     1201 ggaaaatata ttatctgtgt t
//
LOCUS       DAZKFZ010000434         1219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000434 DAZKFZ010000000
VERSION     DAZKFZ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(91..1191)
                     /gene="ompC"
                     /locus_tag="ACHOQW_005022"
     CDS             complement(91..1191)
                     /gene="ompC"
                     /locus_tag="ACHOQW_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9575892.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          343 a          275 c          255 g          346 t
ORIGIN      
        1 tatcaaaaat tgctcatttt tacttaagga gtcatacagc agcacttaaa tcaaaaacca
       61 gcccagagaa gggctggtca ggagaggatg ttagaactga taggtaatgc ccgtagcgac
      121 gatgttatcg gtgcgaatat ctacggcacg ggtaaagtcg ctttcatcaa tcaggttgat
      181 tttataatca acataggtcg acatattttt gttgaagaaa tacgtcgcac caacgtcgat
      241 atactcaatc atgtcctgat cgccgtagcg gcccagatct atgcctttcg atttcaggta
      301 agcgagtgag gggcgcaagc cgaagtcgaa ttgatattgc gccaccactt caaaactttg
      361 tgctttgtta gaaatataac catcgccata ggtggtcatg tttaatgttt cagcgtaatt
      421 agctgcaaag tagatgttgt tggcatcata ttttatacct gtagcccaca gttctgcatt
      481 cttaccggag gcattgagag gattagcagc ggctctttcc tgcgcatttg tccgatcgga
      541 tttggtatac accgctccga taccaaagcc atcataaaca taagttgagg agataccgaa
      601 accgtcaccg ttggcacgcg tgtagtcagc accataaaga tgactgttgt cagtacgttc
      661 atttttgcct tgatattgcg cggcaaagtt cagcccttca attaagccaa agaaatcgta
      721 gttgcgatag gttgctacac cggtggtacg gtatgtcatg aagacatctt tctgactcca
      781 ggtatcgcca ccaaattctg gcaacatatc agtccatgaa ccaatgtcat agccgacacc
      841 gtagttacgg ccatagtcca gagagccaaa ttcactaatt tttaaaccag cataggccag
      901 acgggtcttg ttgtcgcgcg agccgttggc ttcatcgtta tagcctttaa aatcatattc
      961 ccagtgacca aaaccaatca tttgatcatt gatttgcgtt tctcctttaa agccgagacg
     1021 ggcataagtt ttatcaccat cgtcacgctt atcatcagta aaataacgta gagcggtaac
     1081 cttgccataa aggtcgagtt tattaccatc cttgttatag acttcggcag cgtgtgctac
     1141 gttcgcagct aacaaaccag ttacaaccac cgcaactatt tttaatttca tttaacagaa
     1201 tccttttaat tatcgttaa
//
LOCUS       DAZKFZ010000435         1213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000435 DAZKFZ010000000
VERSION     DAZKFZ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1213
                     /locus_tag="ACHOQW_005023"
     CDS             <1..>1213
                     /locus_tag="ACHOQW_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575893.1"
                     /translation="DKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEIRGGEFV
                     DDDFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELSDA
                     SDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAYKS
                     IYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSEIV
                     DHGRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREIHVTYDE
                     SGISAGNKKVTKLLSDLDKISGDIKRSYGVPSNQGIQGSFSIKCINSLTGLVGIPGLP
                     DKEFALSTPEFMKSKQHKAFSTVFKDVTNELTSIERLGGLRDKLASNFTIDDSYYTEP
                     KIEDPRFRYVSS"
BASE COUNT          394 a          192 c          253 g          374 t
ORIGIN      
        1 agataaacgc attgctaaat ttgactttat taaatttgta tctgaaagtg attttcaact
       61 cagtgaactt gatcagttag cacaagttga atctcggcaa attacttccg aaattagagg
      121 tggggagttt gttgacgatg attttaggca acttgtagag atgcttaagc tgaatatgat
      181 ttgcacatgg gatcttcggt ctagcgtgta ttacttaaca atgaaaatgc ttggtcagcc
      241 cggtactcct gaatatgcga aatatagtga actaagtgca tcgattttta atgaattatc
      301 agatgcttct gatacgaaag ggtactggtc aaccgatgtg aagctagtga gttcatctgg
      361 acatgaatat actgagcgtg aatttgaact agaaagggaa aaatctactc gcggtaaagg
      421 aggtatgact cgttcacttg aaatgtttat cgcatcgcta aattggttgg catataaatc
      481 tatttattac agcgtcattg ctaagcattt taaagcagac tcatttattc atccaataag
      541 gcatgcatat caattgcatt ggatgaaaaa aacaggtgct tttggtcatg attatactgc
      601 aaggttggtg caatcgttgt ctgataaaat cagtactgca agatctgaaa ttgtagatca
      661 tggacgcaca tctactattt cattagattt accaatattt tctgcatggc tagcaaacga
      721 aacaggaaat gtctctcaag ttatcaattc agctttagag cttagaacac atgatcattt
      781 tcgtacgtgt cgagatgtaa tccgagagat tcatgtgacc tacgatgaat cggggataag
      841 tgcaggaaat aaaaaagtaa caaaattact aagtgattta gataaaatat ctggagatat
      901 taaacgaagc tacggtgttc catcaaatca aggtattcaa ggcagttttt cgataaaatg
      961 tataaattca ttgacaggac tcgtgggtat tccaggtttg cctgataaag agtttgcact
     1021 gagcactcct gaatttatga agtctaaaca acataaagca ttttctaccg ttttcaagga
     1081 tgttactaac gaactaacaa gcattgaacg tttaggtggg cttcgggata aattagcgtc
     1141 gaactttact attgatgatt cttattatac cgagccaaaa atagaggatc cgagattccg
     1201 ttacgtaagt agt
//
LOCUS       DAZKFZ010000436         1207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000436 DAZKFZ010000000
VERSION     DAZKFZ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1207
                     /locus_tag="ACHOQW_005024"
     CDS             <1..>1207
                     /locus_tag="ACHOQW_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFP9575894.1"
                     /translation="YNSGKNIGSHPDMTDREIMESRAYNFACTKLSDAGEPDIFGWGD
                     KEICNSMELSESQLANGYSNRQWPSQIPVIPEGIYNTVYLGYGETLRANTEVTLSSTG
                     YFYEIARAYTDDELSEPLLTVCNQHSHVIGGKEPLTIIIPAIEHGMLGSNLHMIERFK
                     NYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSDGRKQNF
                     NFHHPSGFYSGKVMSITQKGINAVVGTKLGYDKIRGNNLDNTFSLGSGGGVIYSGGGS
                     NTYFVPATLQDNLHIYISEKSNGNHIILGDMHSRLSIECHFSNNEREFIKMGYYNGCD
                     VILESDTIQKIKSFAKNITIQTADGVMANWDDKLNTLSVYSIDMIAWRDKNKTAKEPL
                     PVDVIQLTNW"
BASE COUNT          420 a          170 c          248 g          369 t
ORIGIN      
        1 tataatagcg gaaagaatat tggtagtcat cctgatatga ctgacagaga gattatggag
       61 tctagagcat acaattttgc atgcacaaaa ctctcagatg caggtgaacc cgatattttc
      121 ggttggggag ataaggaaat atgtaattca atggaattat cagaatcaca attagctaat
      181 gggtattcta accgccaatg gccttctcag attccagtta ttccagaagg aatttataat
      241 acagtatatc ttggatatgg agaaacattg cgagctaata ctgaagttac attgagtagc
      301 actggttact tttacgagat tgcaagggca tatactgatg atgaactatc tgagccatta
      361 ttaacagtat gtaaccaaca tagtcatgtt attggtggaa aagaaccatt aacaattatt
      421 attcccgcta ttgaacatgg tatgcttggc tctaatctac atatgattga acgttttaaa
      481 aactataatt tctcgatatc aggagggaag ggagggataa aattaatggt tggtggtatt
      541 ggtgattaca acatagaatg tacccccggt gttagaaata ttatatcgtt tgaacaatta
      601 tcacgtgact tcaatctgga tttggattta tcagatggga ggaaacaaaa ttttaatttt
      661 catcatccct caggttttta ttcaggaaaa gttatgagca taacgcaaaa gggtataaat
      721 gcggttgttg gtactaaact gggatatgat aagattcgtg gtaataatct tgataatacc
      781 ttttctcttg ggagtggagg aggtgttata tattctggag gagggagcaa tacctatttc
      841 gttcccgcca cactacagga taatttacac atatatatat cagaaaaatc aaatggcaat
      901 catattatat taggtgatat gcactctcgc ctatctattg aatgccactt tagtaataat
      961 gaaagagagt ttataaagat gggctactat aatggatgtg atgtgatttt agaaagtgac
     1021 actatacaaa aaataaaatc atttgcaaaa aatataacaa tacagacagc ggatggagtt
     1081 atggcaaatt gggatgataa actaaatact ctgtctgttt attcaataga tatgattgca
     1141 tggagggata aaaataaaac tgcaaaagaa ccgttaccag ttgatgtaat ccagttaact
     1201 aattgga
//
LOCUS       DAZKFZ010000437         1204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000437 DAZKFZ010000000
VERSION     DAZKFZ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(22..249)
                     /gene="mchB"
                     /locus_tag="ACHOQW_005025"
     CDS             complement(22..249)
                     /gene="mchB"
                     /locus_tag="ACHOQW_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFP9575895.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(277..486)
                     /gene="mchI"
                     /locus_tag="ACHOQW_005026"
     CDS             complement(277..486)
                     /gene="mchI"
                     /locus_tag="ACHOQW_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFP9575896.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            595..831
                     /locus_tag="ACHOQW_005027"
     CDS             595..831
                     /locus_tag="ACHOQW_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575897.1"
                     /translation="MIKDNSKVDDSADRLSFTETLFVAHSILPDLTQTKTKENDCYLL
                     FKKKCHIVHIKSRGILRYVTDAIVSQDLMVVAHV"
BASE COUNT          406 a          257 c          221 g          320 t
ORIGIN      
        1 gtaaaataaa taaaaatata attagctacc gccaccagca gaagaactgg cactaccact
       61 tcccacggtc gagccaattg ctgttgtcaa accggcagat acggctccaa ctacaacccc
      121 aagtggacca ccaggtattc cggcgagagc tccaacaata gctgcagcac ctgcagcatt
      181 agctgaagtc gctggcgcac ctcccgcccc ggaaatatat cttaactgtg attctgttat
      241 ttctcgcata taaactgcat ataaactcca tcatatttaa cttccgtttt tccagcctga
      301 ccagaccaga atccttcgaa tcccatgaat cagctcaata aaaccaagaa atattataaa
      361 gcttagaaaa acgtcaacat aagaattccc ttcgccaaca atccgaaggg atgaaagtga
      421 aaccaataag atagtaatag gtaaactgaa tatagccgtc aattgggaca gttttttata
      481 actcattaat ccctacctgt tgctttattt tcattatgaa aagttaccaa atcttagcac
      541 ctcagtcagg agagtcaggt catcgataaa gaatattcca gtagagtggc gctcatgatc
      601 aaagataaca gcaaagttga tgacagtgct gaccgactgt catttacaga aaccctgttt
      661 gtagcacatt ccatcttacc tgacctcacc cagacaaaga caaaagagaa tgattgttat
      721 ttacttttca aaaaaaagtg tcatattgtc catataaagt caagaggtat tttacgttat
      781 gtcactgatg caattgttag tcaggatctc atggttgtcg ctcatgtata acctgagact
      841 tgaacgcaca ctaataacac tgacggtcat acatcgggaa atctgaggag gcataagaat
      901 gaattgtgat aataatcaca gaaatgaaga attcattgtt acctttgata aaggcaacaa
      961 gcaagacaat tcaagacgaa aacacgataa ttttcctata gaggtagaat cctccgtaga
     1021 gcaggagaca cactgtatca caaataataa gtcggcttcc ggtatagtaa cacatgacta
     1081 tgatgccgat tatatttgtg gttgtggtga aattatgtgt cctggttgcg gtcatgacct
     1141 ataacaaaca tttttgcaaa ctgatacctg atcaggtaca agcctgttga ttattatgct
     1201 gaat
//
LOCUS       DAZKFZ010000438         1197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000438 DAZKFZ010000000
VERSION     DAZKFZ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(464..682)
                     /gene="ydfC"
                     /locus_tag="ACHOQW_005028"
     CDS             complement(464..682)
                     /gene="ydfC"
                     /locus_tag="ACHOQW_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9575898.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(754..1053)
                     /locus_tag="ACHOQW_005029"
     CDS             complement(754..1053)
                     /locus_tag="ACHOQW_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575899.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSDECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          273 a          313 c          281 g          330 t
ORIGIN      
        1 tgtttttggt tttaactgtt ccgcgtgcgc tcagccgcat tcaccgcatc acaaaattca
       61 ctttaaaaag ggcggcagag cagtcacgga gtaaaactga taccgccaaa cgtcaccaga
      121 aaattgataa cagagggcgt tgcagcgggg ttgtcactta agcgtatggt caacctgaca
      181 acccggtgtc ctcaacgggg gaaggaataa cccctccata cttaccgccg cgccatttcg
      241 cggattgcca caaccggaag cgcacggtcg acgaaaattt aacgacaggc tatctatgaa
      301 ccagctacct cgccgtgcgc tttcgcgtta tggtctgact tttcagggaa atatcctttc
      361 agtaaactgt cagtgccgta tgctcacccg tgtccggcgc acgcactcca cctcacccgt
      421 ggagaactcc ttaattacca accttagctt cgttggttag ctattaacgc gggtatgtaa
      481 tcattctggc aatgcttaat gccgctgctt tttccagatt ggtgatatcc tgctccagag
      541 cggacagatt ttcagcctgc ttagccctgg cttcattggc ccatttcaga tcctgcgctg
      601 cattaatttt ctggtgcatc cactcataaa gttcatcatc ggtatagtct ggcgcgatga
      661 tgacgggttc tcgtttctgc atgtcggctc cttgtggtta gcgttgcctg cttttaacca
      721 cgtcaggcga ggtggtatcc tctgaggggt ctgttactcg agaggaaatt ggttatgaat
      781 acaatcaagt tttcttgccc agaatgtggt ggcgaagtct ttgacacatc ctttaagccg
      841 cagggctctg acagtttcgc gggagccatc tgcaaaaatt gtggtcacct tgtaactgaa
      901 gatgagtcct cgcagttcga tgacgaaatc gttgacaata tcttcggtgc actcatcaga
      961 gactttctga agtaaaggcg cataccgctt agttaccgct ctgataactc ttacctgtcc
     1021 tgcaatggcg ctgatgtcaa tataaagcgc cattgctgct tctttgccga tcccgggatg
     1081 ccttccattc tgatgtttga cttcgcccac tgagaaaccc tctgtttccc cttaacgccg
     1141 gggtagcgga acaaaaacct gctgcatagt tattaaagtt gaaccctgcc gtcatgt
//
LOCUS       DAZKFZ010000439         1185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000439 DAZKFZ010000000
VERSION     DAZKFZ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..178)
                     /locus_tag="ACHOQW_005030"
     CDS             complement(<1..178)
                     /locus_tag="ACHOQW_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575900.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAA"
     gene            752..940
                     /gene="dicB"
                     /locus_tag="ACHOQW_005031"
     CDS             752..940
                     /gene="dicB"
                     /locus_tag="ACHOQW_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9575901.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            937..1128
                     /locus_tag="ACHOQW_005032"
     CDS             937..1128
                     /locus_tag="ACHOQW_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9575902.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
BASE COUNT          336 a          251 c          275 g          323 t
ORIGIN      
        1 gggcggcgtt taactcttta ttcagtaaat cagactgata ctgatttggc tttgtaatga
       61 tactgtcaag ttttgatata tgaaattcaa catgttctgg acagttaaaa atgtaataac
      121 cgcccttctc taaaaagcca gaaagagcgt tacaggcagg gcatggtttg tttggcattt
      181 tatcctccat taagggctga attaaaaatg gagaccaaca cgctgctacg tgtggtcgtg
      241 cgccggacac agataagaat ccggcactga cagtttactg aaaggatata tccctgaaaa
      301 gtcagggcat aacacgaaag tgcacggcga agtccttctc ccttagagcc gtcgttaaat
      361 ttaattcgac cgtgcgcttc cggttgtggc aatccgcgaa atggcgcggc ggtaagtatg
      421 gcggggttat tctttccccg ttgaggacac cgggttgtca ggttgaccat acgcttaagt
      481 gacaaccccg ctgcaacgcc ctctgttatc aatattctgg tgacatttgg cggtatcagt
      541 tttactccgt aactgctctg ccgccctttt ttaaaagtga attttgtgat gcggtgaatg
      601 cggctcagcg cacgcggaac agttaaatcg gtaaagcggt catttgctga gtaacgagaa
      661 tgctctgtat ccggcgttaa ttgttaactg gttaacgtca cctggaggca ccaggcaccg
      721 catcacaaaa ttcattgttg aggacgcgat aatggaaaag ttatcatgta atgccagcac
      781 gtctgaactt cgtttcgaaa ttggcgttat cactggagac aaaacattta ttgaagacgc
      841 cattaagcag agaaaactcg agcaggacct gttaaatgaa gtatgcattc cttcaatgct
      901 ggctcgtctg gacctgctgc aaaaaggata taaacaatga atacaacatt tgcactcgtt
      961 ctgacagttt atcttgtttc cggcgaatct cttgagctgg tgactggctt atacggttca
     1021 atgaaagaat gcatggctgc agcagcagaa caaaaaattc ccggtaactg ttatccggta
     1081 gataaaacta ctcacactaa taataacgaa ataccggcag gactttaaaa cagcaccgta
     1141 attaatatcc ggtttcattt ttatatgcca gcaatggcag ggatt
//
LOCUS       DAZKFZ010000440         1180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000440 DAZKFZ010000000
VERSION     DAZKFZ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..253
                     /locus_tag="ACHOQW_005033"
     CDS             <1..253
                     /locus_tag="ACHOQW_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9575903.1"
                     /translation="SGAVIRGVFDDPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRG
                     DTLTINGEMFWVDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            268..801
                     /locus_tag="ACHOQW_005034"
     CDS             268..801
                     /locus_tag="ACHOQW_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575904.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            798..>1180
                     /locus_tag="ACHOQW_005035"
     CDS             798..>1180
                     /locus_tag="ACHOQW_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9575905.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          280 a          274 c          359 g          267 t
ORIGIN      
        1 gtccggcgca gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca
       61 gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca
      121 actgcggcgt ggtgacacgc tgaccattaa cggcgagatg ttctgggtgg accgtgtttc
      181 tccggatgac ggagggagtt gttatctctg gctcaaccgt gggcaaccac cggcagttaa
      241 ccggcgacga taaacgcagg gtgaattatg gcgataaaag ggcttgatca ggcgattgaa
      301 aatctgagcc gggttcgtaa aaacgccatt ccggcggctt cagcaatggc cattaaccgc
      361 gtggccacaa cggcgattaa tcagtcttca tcacaggttg cccgggagac aaaggtacgc
      421 cggaaactgg taaaggaacg gtccagactg aaacgggcca cggtcagaaa tccgaatgcc
      481 agaattatcg ttaaacgcgg tgatctccct gtgattaagc tggggatcag gatgctgggc
      541 cgtcgtccgg acagcatact taaagctggt cagcatcggt atcagcgggc atttatccag
      601 cgattaaaaa atggtcgctg gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc
      661 attgatgtgg tgaaaatccc gatggcggcc ccactgaaac aggcgtttga tgagaatatt
      721 gaccgtatcc ggcgtgaacg tctgcccgga gaactggcat acgcgctgaa acaacaactt
      781 aggattgcga taaaacgatg aaacatactg atatccgtgc ggcagtgctg gatgcactgg
      841 agctgcatga acacggggcg acgctgtttg atggtcgccc cgttgttttt gacgaagagg
      901 attttccggc cgtcgcggtt tatctgacgg atgcagagta taccggtgaa gagctggatg
      961 cagatacctg gcgggccaca ctgcatattg aggtgttttt accggcacag gtaccggatt
     1021 cggagcttga tcagtggatg gaaagccgga tttatccggc ggtgactgcg atcccggcac
     1081 tggcagacct gattacgacg atggttacgc agggctatga gtatcgtcgt gatgacgata
     1141 tggcgttatg gagttctgcg gatctgactt attccattac
//
LOCUS       DAZKFZ010000441         1176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000441 DAZKFZ010000000
VERSION     DAZKFZ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.445"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            117..224
                     /gene="yniD"
                     /locus_tag="ACHOQW_005036"
     CDS             117..224
                     /gene="yniD"
                     /locus_tag="ACHOQW_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP9575906.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(277..1035)
                     /locus_tag="ACHOQW_005037"
     CDS             complement(277..1035)
                     /locus_tag="ACHOQW_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP9575907.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          328 a          284 c          275 g          289 t
ORIGIN      
        1 aaacagtgat caggtgtaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg
       61 cctgtccctt ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc
      121 cgactaaacg ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg
      181 gcgctatgtt gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag
      241 acagccagac cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa
      301 ggttgtccga cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac
      361 gttgtaagcc accttcagtc caaaatgctc attaatagca acatttaatg cgctttcgga
      421 gttcagcgtt gtgtcttccg cgccaaagac ggaaacaccc tgcgtaaatt tagcgttgtc
      481 agtaagctgc catgcataag caccggaggc atagcccagc ggctgagttt cactggcatt
      541 gtcggtatat ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg
      601 gccattcaaa aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt
      661 ataacggtct gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa
      721 acggctacgc ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt
      781 actggcgttc ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc
      841 agccgtaagt gaggagcttt tcgtattgcc tgattgtgca agataccctg cgttcagatt
      901 accttcgaaa ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc
      961 ggcagctgca ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt
     1021 cttcaaaagc ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg
     1081 gaaactttct taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc
     1141 gcgaataact tatcaatgta ttcttatttc attttt
//
LOCUS       DAZKFZ010000442         1176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000442 DAZKFZ010000000
VERSION     DAZKFZ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.446"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>1176)
                     /locus_tag="ACHOQW_005038"
     CDS             complement(<1..>1176)
                     /locus_tag="ACHOQW_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9575908.1"
                     /translation="RRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALI
                     SEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGR
                     YITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSG
                     DLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPTSG
                     VSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTWQECWV
                     "
BASE COUNT          255 a          382 c          247 g          292 t
ORIGIN      
        1 gacccagcat tcttgccacg ttctacaagc cagagtcata actgaagcgt caacacctgc
       61 accgatacca acagattctg tcgatgattc tggattatca acatccacta cataagtata
      121 cgcaaaatgc cccccaactc cgccgccaac cgttacacct actgaattgc tgactcccga
      181 tgtaggtaaa gacttggtct cagaatatgt gatagccgca tttatcccac caccaattcc
      241 tgcatgtccg catgctgtag cataaataca aatatcagat aacatttcac ctttcttttt
      301 agcaaacgca acgccaagtc ctgcatgacc acctaatgat attagatctc cactgccagt
      361 aatatcaatt gatgatgttc catatgaatc atcatgccaa acatcatata ataattgata
      421 taaattatac aaacccaaag gatccatcct tattatcgga ttcagtgggt aattatacag
      481 attccatccc cccgccagcc caatggggtc ttgtgtaata tacctcccct gtagcgggtc
      541 atagtaccgg ttccggttat agtacagccc tgattcctca tcatgctgct gccccggcag
      601 acggtatggc tggtgcaggt aatacgggtt ctcttcatta agctggttgc cccattcatc
      661 atactccccg cgccacgccg tattgccgtc ttcgctgatg agcgccagcg gcaggccccg
      721 gtggtcgcag tggtaaaaat gaacttttcg cgccggtgtg tattccggct ccacctgtct
      781 ggccagttgc tccaccgtca gcccgcactg cgcaagccac gcccggcttt cactgctcac
      841 gcggtctgcc cggatttctt cctccagcct gtccagcagc cgcaccagtt cagccgggaa
      901 caccacgccg tggccgttct cactcccttc ctgctggagc gtctctgcca ggctgcgccg
      961 ctgcgctttt tcccgctcgc cgttctctgt ctcgacccgg atgagcggcg tgaagcttcc
     1021 cggctcgtat accgtctgga tacgtgtggt gtcagtctgc accgtcgtca gcctgtctcc
     1081 gtcccagcca taccacgtca cctccggttt acgcgacagc gacatccacc ccgtcaggtc
     1141 acgctcccgc cgccagaccc gttttgccat tcgccg
//
LOCUS       DAZKFZ010000443         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000443 DAZKFZ010000000
VERSION     DAZKFZ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(95..444)
                     /locus_tag="ACHOQW_005039"
                     /pseudo
     CDS             complement(95..444)
                     /locus_tag="ACHOQW_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            538..801
                     /locus_tag="ACHOQW_005040"
                     /pseudo
     CDS             538..801
                     /locus_tag="ACHOQW_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            841..961
                     /locus_tag="ACHOQW_005041"
                     /pseudo
     CDS             841..961
                     /locus_tag="ACHOQW_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            958..1110
                     /locus_tag="ACHOQW_005042"
                     /pseudo
     CDS             958..1110
                     /locus_tag="ACHOQW_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          313 a          285 c          285 g          287 t
ORIGIN      
        1 attgaagtat atctatctgt catttttgca aatgacatgc ggtagcataa cgttccgggg
       61 gctcctgtgc cgtcatgcgt gacgacattg atcagccaat aatggcacgt gaccacagcc
      121 agatacggcc agccttcatc cgtacgtaaa tatgtgatgt ctcccgccca cttctggttc
      181 gggccactgg cgtaaaaatc ctgctccagc agattttctg acacaggctg gctgtgtgcg
      241 cggtaataga ccggactgaa attccaggaa gcttttgccc tcagtccgtg acggcgcagg
      301 ctttccgtca cggtttttac tttaaaatgg taaccctggg cacgcagttc atccatcagg
      361 cgttgtgcat gaccgggtaa aagcaacgag gacaacgctg tcgcagtgct gacggaactg
      421 ccgacgtgta cttatcctta tccgccagtg atataagacg gtaattcacc atttggattg
      481 tccgctccac ccaacatgtt gtttccttga ggttctcaca ccagaaagga catcagcatg
      541 ctgagcagag aggactttta catgataaag caaatgcgcc agcaaggtgc gtatattgtt
      601 gatattgcga ctcagattgg ttgctctgaa cgaacggtca gacgttacct gaaataccca
      661 gaacctccgg ccagaaaaac acgccacaaa atggccgaaa tggactatat cgacatgcgt
      721 ctggctgaga atgtctggaa cggcgaagtt atcctggcgg aaatcaaagc gatgggttat
      781 accggtggcc gttccatgtt gcttcgccag ttcaggaaga caccggagca gcgctttacg
      841 caggaacaca gtctgttctt ccggttgctg aaccgtcgat atgaaaaagc gagcattgtg
      901 ctgacgtcca ataaagggtt cgccgactgg gggagagatg ttcggtgata acgtgctggc
      961 aaaatattca cccaacgcat tgagcgtaat aatctgacgc tacgcacccg cattaagcgg
     1021 ttgactcgta aaaccatctg cttctcacgc tcagttgaga tccacgaaaa agtgatcgga
     1081 gcgtttatcg aaaaacacat gttctactaa ttggatgcac caccgaatac attaatttat
     1141 ataacaacat caaaatacat tttaggttgt
//
LOCUS       DAZKFZ010000444         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000444 DAZKFZ010000000
VERSION     DAZKFZ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(29..1153)
                     /locus_tag="ACHOQW_005043"
     CDS             complement(29..1153)
                     /locus_tag="ACHOQW_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP9575909.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          353 a          291 c          211 g          315 t
ORIGIN      
        1 atttctcgtt gaaaatttca gcaatacgct acaattgttt cccgttgttt tttaattttc
       61 tcatcaaaac ctctgcaatc tctgcaggac tgggttgttc agccatttca gcggctacct
      121 gatctgaaca gaagcgtagt gaatcatcat aaagcaggcg atttaccaaa tcactggtca
      181 gtccatgctt gtccggaata atcccacacc cacgcttcgc aacaatttct gcattaacag
      241 agcgatctgc accttcgcca aacacaatct gtggtattcc actatacagc gctgtcaggg
      301 tattacctgc gccaccgtga tgaataaatc catcagctcc attaaggaat acacccatag
      361 gtatccagtc aaccagacgt acatttgatg gtagttttcg taaaccagta cgagcattta
      421 ttgcaagttg caaaatgata tcagcatcaa cttcatttgc agaatccata acccatgaaa
      481 tcagctccag accatcaacc attggtttta cagtgccaag gctgataagt aaacgtttcc
      541 gatcagaatc cctgtcccac catgtttcct ttacagcacc tccgttataa ggaatatatc
      601 tcattgagat aaccggttcc ccagcatttt taagaatgct catacttggg ggagcaacat
      661 caatccatgc taaatcacat agtggaccgc tgactccatg ccgcctgtaa gcattgctta
      721 aagaacggtt taacatctga atatgggcag atgtatgcgc gaatccaaca gccagcatca
      781 ctgaaggaat tctatattta gcagcaacca atgggcctgc cggaccaaga gggggataga
      841 ctattaaatc tggcctccat tttcctgcaa aatcgatgag gttatctgcc atttcatcgc
      901 taaaaaaaga gaaatgacca ataatattac tttttttcct caactcttcc tgatggcgat
      961 atccagcctc tgaatctaaa cctggaactg catcaaaaac aaccagtcca gcttctgctg
     1021 ctttattcgc gaatttgcca gcgctactaa ttactacatc atgtccaatt acacgaaagg
     1081 cctgagccag agaaatcaat gggtataaca aaccgtacag cggtgggcca ataaaaagaa
     1141 tacgttttcg cattaaaaaa gtccgtcttc
//
LOCUS       DAZKFZ010000445         1161 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000445 DAZKFZ010000000
VERSION     DAZKFZ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            535..825
                     /locus_tag="ACHOQW_005044"
     CDS             535..825
                     /locus_tag="ACHOQW_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9575910.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            849..1100
                     /locus_tag="ACHOQW_005045"
     CDS             849..1100
                     /locus_tag="ACHOQW_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9575911.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
BASE COUNT          320 a          212 c          279 g          350 t
ORIGIN      
        1 tttctcatcg aaagcaagtt tgtcaatata aatttgtaac agaaatgact ttccggggat
       61 aaacaaaact gttcatggca atggctttct ctgtcgccaa catggataca ttgttccgga
      121 taaagcactg atgtcattga ctgacgcaga ctatcaggcc atctgatgct ttcttttcct
      181 tcaccggtat tgatgatatg ccttatgaag ggggacttat tcctgtaacc caatcacagg
      241 cactgtgatt actggtttat gattgatgct gatttgagca gatatgcaca gcagttgtgg
      301 attcagggga tattttctgc cagatattca taccagattg atgacctctt tgctcagatg
      361 ctcttcgttc tgagccagcc aggattttgt ggccacatac attgttcgcc gatggggaat
      421 cgagacaggc gattggctgt gatgcgattt tgccgctaac tgaggatgag tccgcccctc
      481 ccggggaatt cctgtaagtt aagccgtacc tgttgattag cactaaactg ggtaatgaat
      541 ttatctgcca gtatgaatga tgccggggac ggttttgaac tcctgatgtc gggtaatatg
      601 caggatttta atatttgttg tcgtcgggat aatcattatc ctgaacataa actgatacct
      661 ttatcagagt tatttgacgt taattatgga ctgaatcttg agctgaataa actggagaaa
      721 gattcttcag gaattaattt tgtttcaaga acgtccaaaa ataatggcgt ctccgccagg
      781 gttaaattaa aagatggtat tagtccatgc cagcagctgt gttaactgta gcagcaggag
      841 gttctgttat ggaatcgttt gtacaggatt caccatttta tagtggaaga gatctttatt
      901 ggttacgtcc taaagttgaa ctgactctgg aagagaaatt gtactactgc tcttgtataa
      961 gaagaaacag acacaaatac agctacgggc gtcaggcaaa ccgaacgctg aagaatttac
     1021 tggtaccgtc attagattct gttccggcat gggtatatgg tgtgacaggg aaaataatat
     1081 ctgaattgtc tgaacgttga gactaagagg gccggaggcc aggtgaatgg ccaccgggat
     1141 atttatatca gagtatattg g
//
LOCUS       DAZKFZ010000446         1160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000446 DAZKFZ010000000
VERSION     DAZKFZ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..283)
                     /locus_tag="ACHOQW_005046"
     CDS             complement(<1..283)
                     /locus_tag="ACHOQW_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP9575912.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYT"
     gene            complement(918..>1160)
                     /locus_tag="ACHOQW_005047"
     CDS             complement(918..>1160)
                     /locus_tag="ACHOQW_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9575913.1"
                     /translation="IYKQLRYLEKSASQGNVTAQFNYGVFLSDTNPTLSEYYNLNRAI
                     YWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          342 a          242 c          195 g          381 t
ORIGIN      
        1 atgtatagga ttcggaaagt ccagcatcta ttttgtatct cattatatta gataaggatg
       61 tgagaaattc cttgttatca ttcgcagcca tatatgccag tccgagttca gcctctgcgg
      121 ccttacaggc aaaatagctt agtgaacacg cttttcgtcc ctctttagta ctggctgata
      181 ttagtgccca taatattgct ttgtctgcca aatctaactc tctctgaaat tgtcttgcag
      241 taactgcact cgcagaaata gagaaaaaaa tacaattgtt aataaccttg ccatattttt
      301 tagtccgtaa ctgttaatgt gctatctggc tctgaaaata ttaactcatt taatttttgc
      361 atgtcattag ataaaccagt tatacaaccc tcggaacaga atcgaggtcc taaagacgga
      421 ccaaaagcgt ttaaacaact atgtattgaa aatccattgc gattataagt tcctgtatta
      481 actgacggta ctaatcgtat gccgtcaggt tttctattgc ttgctcccgg gtcattaaca
      541 ttccaactcc ataggccttg agatattggc catctattta taaatgtgta caacggggat
      601 tatttttaca ttgcatatta ttaccagaag cgactggaat actaagcaca tcataaaaat
      661 tatcatcaca tggtatatct gaaacacacg aaagcatacc taaagttatg ctgtataaac
      721 aggtggctaa tcccaagggg atcgattcca gaaatcgggt tcaacggata ctgataaaaa
      781 ttccacctac cttccagccc aatcggagcc tgagtgttat accgcccctg ctgcggttaa
      841 tgttaccgat aatagttata gtacagccct gattcatcat catactactg ctgcggcagt
      901 ctgatgaact attgctttta cggattctcc ttattcttcc ttctatccat tcgctttagc
      961 tttttgagct cttgaagttt gctctttgca tcaatattac cgttatttac agcgaattcc
     1021 atccaataaa tagctctatt caaattataa tattctgata atgttggatt tgtatctgat
     1081 aaaaagaccc cataattaaa ctgcgctgta acattcccct gagatgctga tttttctaag
     1141 tatcgcaatt gtttgtaaat
//
LOCUS       DAZKFZ010000447         1158 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000447 DAZKFZ010000000
VERSION     DAZKFZ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            125..364
                     /locus_tag="ACHOQW_005048"
     CDS             125..364
                     /locus_tag="ACHOQW_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFP9575914.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            384..716
                     /gene="sctI"
                     /locus_tag="ACHOQW_005049"
     CDS             384..716
                     /gene="sctI"
                     /locus_tag="ACHOQW_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP9575915.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            713..>1158
                     /gene="sctJ"
                     /locus_tag="ACHOQW_005050"
     CDS             713..>1158
                     /gene="sctJ"
                     /locus_tag="ACHOQW_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFP9575916.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVL"
BASE COUNT          377 a          204 c          241 g          336 t
ORIGIN      
        1 gtaagattta atgtgaattt gaaaaatcag gacacaaata atagttcatt tagctacgga
       61 gataaccgat tcgagaagtc tcaaggtagc aagtggacat ttcaggaata attgaggagg
      121 acttatggca gattggaatg gttatattat ggacatcagc aaacaatttg atcagggcgt
      181 tgatgatctg aaccagcaag ttgaaaaagc gttggaggat ttagcaacca atccttccga
      241 cccgaaattc cttgccgaat atcaaagtgc attagctgag tatacattat atcgaaatgc
      301 gcaatctaac gttgtaaaag cgtataaaga tcttgattcg gcaatcatac aaaacttccg
      361 ctaaactgga taaggatttt attatgtcag taagcaatat gccacctatt gatagagctg
      421 agcaatcaac tgctcatgaa atacagcaag cgaaagtgat tgatttaaat gaccgggttc
      481 tcaatcttga caacccggat gataaaatga tctccgcttt tgctaactat gctgtacaaa
      541 ctgaaaattg gcagcaaaat gcgttacaag cattaacgtc tgataaaaaa ggattaactc
      601 ctgaaaaatt attagtcttg caggatcatg tgcttaatta taatgtcgag gtttcattgg
      661 tgggaacatt agcaagaaaa attgtcgctg cagttgagac gttgacccgc tcatgaaata
      721 tatatcatta ttattgttta tcctgctgtt gtgcggctgc aagcagcagg aattactgaa
      781 ccatcttgat caacagcaag ctaatgatgt ccttgctgtt ttacaaagac ataatattaa
      841 tgctgaaaag aaggatcaag gcaaaactgg attctcaatc tatgttgaac caacggattt
      901 tgcttctgcg gtggattggc taaaaatcta caatctcccg ggtaagcctg atattcaaat
      961 atcgcagatg ttccctgcag atgcactcgt ttcatctcca cgagcggaaa aagcaaggct
     1021 atattctgct attgagcagc ggttagagca atctctcaaa attatggatg gtattgtttc
     1081 ttcgagagta catgttagtt acgatgttga tactggcgat agtggtaaaa ctgctttacc
     1141 aattcatatt tctgtact
//
LOCUS       DAZKFZ010000448         1157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000448 DAZKFZ010000000
VERSION     DAZKFZ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHOQW_005051"
     CDS             complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHOQW_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9575917.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVR"
     gene            704..>1157
                     /locus_tag="ACHOQW_005052"
     CDS             704..>1157
                     /locus_tag="ACHOQW_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9575918.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLD"
BASE COUNT          346 a          240 c          223 g          348 t
ORIGIN      
        1 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat
       61 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      121 cggcacgtaa gaggctaacc tagatttgcc gttcatagat tgagcctcag attaatgtta
      181 ggcgtcttgc aggacgcgta atgttaactg gggcttttct ctatctgccg ttggtgttca
      241 tgcccgaggc agatagcctc aagcacccgc agctattcta cttaacttcc cattacctcg
      301 ccaatatgaa atcggtcaga aagacagacg tcccataagg acaacaacgg aaaaagagat
      361 tttgcttgat gctatttacc tctgcaccat gaagttgcgc caaaatccca gtggtgataa
      421 caggaatttg gttgttggtg attggggaaa gaagtacgtt agactgtggg ttagttcatt
      481 gacatgatag ttacctgcct tcaatttcct aagcaagcga tgtaaacgat aatcaatatc
      541 aaatgtattg acaggtaaaa taataacgat gtacctttgc tgataacata tatcagtctt
      601 aaatcataat ggaggtagat aaaataacta aacacacaca acaaattttt tatcagtgac
      661 atagcatatc ttaagcatcc tttaatgctc atggggagaa cgcatgacta aacatatgga
      721 cattcattgt caaacacgca tatttaactc aattaaccat gacaccaaat ataaaatatg
      781 tatttttact ccaaaaaaca ttaaaaaaaa taatgcaact ctttatatac ttgatgggaa
      841 tagtgccaac aattacattt ctgatattct gcctgttatt gatgcactac ccaatccacc
      901 agtgttagtt acacttggtt atgaatcttg gaataacctt agcattcatc gccgtgctta
      961 cgattatact cctgatggtg aaaatgctat tgttgataac tctaaaccag catggatcta
     1021 ctttactggt ggaggaagcc agtctttccg agaattatta ctaactcaga tcatgccatg
     1081 ggttagtact attgctccga acagttccag aatcggtata tgggggcact cgctaggtgc
     1141 tatttttgtg cttgatt
//
LOCUS       DAZKFZ010000449         1157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000449 DAZKFZ010000000
VERSION     DAZKFZ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..157
                     /locus_tag="ACHOQW_005053"
     CDS             <1..157
                     /locus_tag="ACHOQW_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9575919.1"
                     /translation="LVLVISEYIAPLQDAADLDIATDEQTSLLAAWKQYRMPLNHVDT
                     SVSPDIE"
     gene            326..>1157
                     /gene="nleH1-1"
                     /locus_tag="ACHOQW_005054"
     CDS             326..>1157
                     /gene="nleH1-1"
                     /locus_tag="ACHOQW_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFP9575920.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     S"
BASE COUNT          339 a          185 c          285 g          348 t
ORIGIN      
        1 tctggtactt gtaatcagtg agtatatcgc accacttcag gatgctgcag atttagatat
       61 tgcgacggat gagcagacat cgttactggc ggcatggaag cagtatcgta tgccgctcaa
      121 tcatgttgat acgtctgtat ctccagatat cgagtgaccg gtaatacctg cgttatagtt
      181 cgtaaacgtt cgtttgatgg gatgctggaa ggatgaatta gttgccagat acaaaaagca
      241 agagttcatt tctaattttt gttgccatgt tagggggatg tttgttaagg aaatttagat
      301 gggtttattt tgaaggttga aatgtatgtt atcgccatat tctgtaaatt tgggatgttc
      361 atggaattct ttaaccagaa acctgacttc gcctgataat cgtgttttat cctctgtaag
      421 ggatgctgcc gttcattctg ataatggggc gcaagtaaag gttggcaaca gaacatatcg
      481 tgttgttgcc accgataata agttttgcgt tacaagagaa agtcatagtg gttgttttac
      541 taatctgttg cacaggctgg gatggcctaa gggggagatt agcaggaaaa ttgaggtcat
      601 gctgaatgca tcaccagtga gcgctgctat ggaaagaggc attgttcatt cgaacagacc
      661 tgatttacct cctgttgatt atgcaccgcc agagttaccg agtgtggact ataacaggtt
      721 gtcagtacct ggtaatgtta ttggcaaagg ggggaacgct gtagtatatg aagatgctga
      781 ggatgcaaca aaagtcctga agatgtttac tacatctcaa agcaatgaag aggtgacaag
      841 cgaagttcgt tgcttcaacc aatattatgg tgccgggagt gcagaaaaaa tatatggcaa
      901 taatggtgat attattggta ttagaatgga taaaataaat ggagaatcgc ttttaaatat
      961 ttcgtccttg ccagcacagg ctgagcatgc tatttacgat atgtttgata gactggagca
     1021 aaaaggaatt ctttttgtcg atacaacaga gacaaatgtc ttatatgacc gcgcgaagaa
     1081 tgagtttaat ccaatagata tatcatctta taatgtttcc gaccgttcat ggagtgaaag
     1141 tcaaataatg caatctt
//
LOCUS       DAZKFZ010000450         1150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000450 DAZKFZ010000000
VERSION     DAZKFZ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(125..196)
                     /locus_tag="ACHOQW_005055"
                     /pseudo
     CDS             complement(125..196)
                     /locus_tag="ACHOQW_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            190..1150
                     /locus_tag="ACHOQW_005056"
                     /pseudo
     CDS             190..1150
                     /locus_tag="ACHOQW_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          352 a          278 c          269 g          251 t
ORIGIN      
        1 agaagagggc gataacatac atttcaacct tcaaaataaa cctatctaat tattcctaac
       61 agacatcccc catacatgac aacaaaaacc ggagccggac tccggttttt gtgaagctat
      121 cgggttactt catttcgcca atattttccc acttcccgtc agcacgcagg atttgcagcg
      181 gtcttactac acactgaccc gaggacgaaa aaagagttcg ggctgggaag agacagaaga
      241 gtagcaattt cagaagcatt cacaccaaca ttgagctttt cagcgaaagt gggcacgaat
      301 cattgctgga cagggttaaa ggcactgact caatcactct tcatacgtgg cttgatcgat
      361 atgaaacaat cctcagctag agggggagca agccgaaaac attactcgac tacgccagca
      421 aaatcagggc aattcgaaga aaattgccgg acaaaccgct cactgacata tcaacgaaag
      481 aggtggcagc aatgctaaac acctacgtcg cagaaggtaa agcggtttcc gcaagagtaa
      541 tcaggtcaac ccttgttgac gtttttcgag gggcaatagc cgaggggcat gtggcaacga
      601 atccagtaac aacaacccgt gcagcaaagt cagaagtaag gcgctcaagg ctgacagcta
      661 atgagtatgt cgctatttac catgctgctg agcacctccc catctggctg aggctgtcaa
      721 tggatttagc tgtcgttaca gggcagagag tgggcgattt gtgcagaatg aagtggtcag
      781 acataaacga tggtcatctt cacattggac agagtaaaac aggagccaaa attgccattc
      841 cgctggctct aaccattgac gcactcgaca tctcactggt tgatacacta cagaaatgca
      901 gggaggccag cagcagtgaa acaataatcg catcaaccta tcacgaacca ctttctccag
      961 ccacagtatc acggtattta acaaaggcgc gaaatgcatc cgggatctcg tttgatggag
     1021 acccaccgac atttcatgaa ctacgtagtc tgtccgcgag gctatatcgg aaccagattg
     1081 gctacaagtt tgcacaacgt cttcttggac ataaatctga ttcaatggcg gcgcattata
     1141 gggacagtcg
//
LOCUS       DAZKFZ010000451         1150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000451 DAZKFZ010000000
VERSION     DAZKFZ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.455"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(81..1025)
                     /gene="leuO"
                     /locus_tag="ACHOQW_005057"
     CDS             complement(81..1025)
                     /gene="leuO"
                     /locus_tag="ACHOQW_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP9575921.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          347 a          266 c          232 g          305 t
ORIGIN      
        1 cacattcaga ataaaattct acactcaata aaaaatgcag aataaaccag acattcatgt
       61 ctgacctatt ctgcattcag ttagcgtttg caaattgaga ctaattgctc ttccatccac
      121 tgatggcctt tatcacgccc ggcagcttca tgccaggaga gataacaggt tctgctattt
      181 agttttaacg gcagcggtaa tacctgtaat tctaaggatt cagcgaactc ttcagccagc
      241 caacgcggcg caatagcgac caaatgcgtt tgcgacacca cgctaagtac gctcatcatt
      301 gccatgccct gatacgcgat actggcttgc ttatctaccg tgtcatacca aggttgacta
      361 aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt cgttataaac atcatgtttc
      421 agtaacgggc ccttaattgt tgggtgattt ttgctggcta ccagcaccat ttcatcttta
      481 aataatggca cgctggtaaa ttcaggacga tggaagtctt cataactaat cacaaactcc
      541 gtttcctgat aacgcagctg atgttcagtg ttctgattta atgaagactt gaacataaca
      601 tgtatatttg gcgcaatctg ctcaatgtga ttataaatct gcgaggtcag aatactgtct
      661 aacgggctgc aaacacaaag atgaaataca cgttcactgc tcgcgggttc aaaacctgaa
      721 ccaggcaatt cattttgtac tagttgcaat gcctgacgaa ctgaaccaaa aagttgaaat
      781 gcgcgagcag tcggttgaat accacggcca taacgaacaa aaagctcgtc attaaacatc
      841 accttcaggc gtgcaacagc gttactgacc gcaggttgcg acattcccag aacatgagca
      901 gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa cggttaataa gttgagatcg
      961 accatgcgta gctgtggctt gctgaactcc gccgtctctg gatgatctgt ttgtacctct
     1021 ggcatactta actccactgt cacacttaac tccctttccc ttattggaat gcagagaaat
     1081 aatcctcgag aatatgattt atcatgcata taaaataaga aaaaggaaaa tgagtaaaat
     1141 taggaaaaca
//
LOCUS       DAZKFZ010000452         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000452 DAZKFZ010000000
VERSION     DAZKFZ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..441
                     /locus_tag="ACHOQW_005058"
     CDS             <1..441
                     /locus_tag="ACHOQW_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9575922.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="ACHOQW_005059"
     CDS             597..779
                     /locus_tag="ACHOQW_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575923.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="ACHOQW_005060"
     CDS             792..923
                     /locus_tag="ACHOQW_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575924.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..>1148
                     /locus_tag="ACHOQW_005061"
     CDS             931..>1148
                     /locus_tag="ACHOQW_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFP9575925.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDVAALDARYSRELA"
BASE COUNT          288 a          237 c          341 g          282 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagc
//
LOCUS       DAZKFZ010000453         1145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000453 DAZKFZ010000000
VERSION     DAZKFZ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.457"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..389)
                     /gene="ybfC"
                     /locus_tag="ACHOQW_005062"
     CDS             complement(<1..389)
                     /gene="ybfC"
                     /locus_tag="ACHOQW_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP9575926.1"
                     /translation="MIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFPVLNNKAMWR
                     WYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGTLQELINTAE
                     KTAFLGDRFRSYIRAGIYQKKSSDPAQ"
     gene            complement(414..>1145)
                     /locus_tag="ACHOQW_005063"
     CDS             complement(414..>1145)
                     /locus_tag="ACHOQW_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9575927.1"
                     /translation="LISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          286 a          268 c          225 g          366 t
ORIGIN      
        1 aactgagctg gatcactgct ctttttttga tagatacccg ctcttatata acttctaaac
       61 cgatcgccga gaaacgccgt tttttctgct gtatttatca actcctgcaa cgttcctttg
      121 atggcatgat tttcattaag gcgtagtgac ccgacacgaa ttaagagtct cgccccgctt
      181 ggtgtgaatt tattattttt acatgtaccc agttctgttt gccaataata ctcaccgatg
      241 tcctcattct gataccatct ccacatggct ttattattta agacaggaaa cgcggaaaaa
      301 atagtaccct tagcgaaaat agtaaaacaa tcatgatctt tagcttgtat ttcgcaatca
      361 gctataacac caaaactaac aaatatcatc agcaaaaaaa aacaaaactc ttttcatttt
      421 cttattcctc tcgatgttct ctgtttcatt ttagctaaaa tttcacccaa atcatctatc
      481 cctgctgttg ctggcccatc gggtttatta atgtcaggga ataaataggg ttctgcacaa
      541 gtatatcctg gtggtaattg agttgttggc caatttgtct gtggcaggta atcacttgct
      601 gtctttgtat ccatccctgg atactgggga tcatcctgtg gcgtacaagt cagatttggt
      661 tttacccact tcctgcaata cagtgtgtag tcgtaagggc ttaatgctct gaagaatgtt
      721 ttgaaaacat taggcgcact actggcctgg tcaataggat atggcagtct taattgtttc
      781 gctatgtctg gaaatttact tggatcgggc gtaggcggtt tgttgggtct cgctccatat
      841 ccgccattat ttaagtgtcc atattttgat gccagtccca ttgaatctat atactgaacc
      901 ggattcaacg gatactgata aaaattccat ccccccttca gtccaatcgg atcctgagtg
      961 atatacctcc cctgcagcgg gtcataatag cggtggcggt tgtaatacag gccggactcc
     1021 tcatcatact gctgccccgg caggcggata agctgctgca gctgatgcgg gttctcttca
     1081 ttcagcaggt tgccccactc atcgtattct gcgcaccatt ctgttgcccc ttccttactg
     1141 ataag
//
LOCUS       DAZKFZ010000454         1141 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000454 DAZKFZ010000000
VERSION     DAZKFZ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..458)
                     /locus_tag="ACHOQW_005064"
     CDS             complement(<1..458)
                     /locus_tag="ACHOQW_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9575928.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLREIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLA"
     gene            complement(458..787)
                     /locus_tag="ACHOQW_005065"
     CDS             complement(458..787)
                     /locus_tag="ACHOQW_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575929.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(784..>1141)
                     /locus_tag="ACHOQW_005066"
     CDS             complement(784..>1141)
                     /locus_tag="ACHOQW_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9575930.1"
                     /translation="GGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYA
                     EGGYVGGAGSPAQMRRTEGINFNQNNHVVIQNDGSNGQAGPQLMKAVYDMARKGAQDE
                     IQAQMRDGGVFSGGRR"
BASE COUNT          229 a          424 c          251 g          237 t
ORIGIN      
        1 gccagcacaa acgaggctgt catggccgtc agctctgaca tctgctccac cacccagcgg
       61 tcgctcagct cctgctccgg gtccgcttcc ggattgcccg ccacaaaatt caccgcatcc
      121 agaaaacggg catacacccg gcggcggacc accgtggccc ccaccaggct ctgcaggtcc
      181 tccgccatcc cggtgaccag accaaacaga ttggacaccg tcagcgacgg tctggcactg
      241 ctgcccttcc cgttcatctc aaaaccgctg ccgtcaatcg ggtatacctg atattgccgc
      301 ccctgccagg tgaccgcctc ccctttttca ttcagctcat tacagaaaaa ataccgctca
      361 ccgccctgca ccgtcaggtc aatttccctg agcaccaccc gcggtgactg ctctgattta
      421 accgactcgt tcagactttc ttcgtgaata tcctgcatca gttcaccacc tgcttaaact
      481 cggcactgaa ttcaacccgc agcatgcgaa cccgtgatga ccaggcggca caggtcacct
      541 ttatctgccg ccaggcataa ggcggtgtcc acagaaacgc cttccagcca ccgtgctctg
      601 ccaggaatgc ctccagatgt cgggcctcct cccgggtcac ggaaagcgtc acacggtatg
      661 ttttcaggtc agcattcagc cccgccgcca tacgctgcga atacccgtca ccaaaacgca
      721 cttcacgcac cgatggctgc gagttcacct ccatatccgg cttcactttc cagcgaaatg
      781 ttttcatcgc ctgcctccgg aaaagacgcc gccatcacgc atctgcgcct gaatctcatc
      841 ctgcgccccc ttgcgggcca tgtcatacac cgccttcatc agctgcggcc ccgcctgtcc
      901 gttggagccg tcgttctgaa tcaccacgtg attgttctga ttaaaattaa tgccttccgt
      961 ccgccgcatc tgcgccggac ttccggcacc acccacataa ccaccttccg catagccccg
     1021 catcaggcgg tacaggttgc cgacgccaat ccggctggtt gcttccttcg tgaagacaaa
     1081 ctccccgcgg tgaactatcc ccgcaggctc atatttgccg cccgtccccg taaatcctcc
     1141 g
//
LOCUS       DAZKFZ010000455         1136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000455 DAZKFZ010000000
VERSION     DAZKFZ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1136
                     /locus_tag="ACHOQW_005067"
     CDS             <1..>1136
                     /locus_tag="ACHOQW_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9575931.1"
                     /translation="CAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQFLEHYPA
                     AQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEGAASFVPVETESDPMNVAFDK
                     LAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPE
                     LKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARARVHRQH
                     TENSAEKIPPVTAVIRREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVID
                     NDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGV
                     FEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSV"
BASE COUNT          327 a          284 c          291 g          234 t
ORIGIN      
        1 tgcgccgcca ttccggacga acaggcaata aaggaagaag gtgcagtcgc tgtagccact
       61 gccattgaag ccggtgatga acgtcgcgcc cgcgcaaaat ttcactggca attcctggag
      121 cattatccgg ctgctcagga ctgcgcttat aaatttcttg tttgcgagga taaacccggt
      181 ataccccgcc ctgccctcga ttcctgggat gctgaatata tgcaggaaaa ccgctgggat
      241 gagggggctg cttcctttgt cccggttgag actgaatccg atccgatgaa cgtcgctttt
      301 gacaagctgg cccctgaagt acagaacgct gtcatggtta agttcgacac atgtgaaaac
      361 atcaccgttg atatggtgat tagcgcgcag gaattgttgc aggaagacat ggcaacattc
      421 gacggacata tcgttgaagc gttgatgaaa atgccagaag ttaacgccat gtatccggag
      481 cttaagctgc atgccatcgg gtgggttaag cataaatgta agcctggtgc caaatggccc
      541 gaaattcagg cagaaatgcg catctggaaa aaacgtcgcg aaggtgaacg caaggaaacc
      601 ggaaaataca cgtctgttgt tgatctcgcc cgcgccagag tccaccgaca gcacactgaa
      661 aactcagcag aaaaaatccc ccctgtcact gcagtcattc gtcgcgaata taagcagaca
      721 tggaaaacac tggatgacga actggcctac gctctctggc ctggtgatgt ggatgccgga
      781 aacattgacg gcagcatcca tcgctgggca aaaaatgaag ttatcgacaa cgaccgcgaa
      841 gactggaagc gtatctcggc atcaatgcgc aaacagcctg atgcccttcg ctacgaccgc
      901 cagactattt ttggccttgt ccgtgaacgt ccgatcgaca ttcacaaaga ccctgtggca
      961 ctgaacaaat acattactga atacctgact acaaagggcg tgtttgaaga tgaaggaaca
     1021 aatcagagcg caactgacac tctctcgtcg ccagtaccag aaactgatgc agtggaaacg
     1081 gcaattccgg acaacgaaaa aaccgaatgc aaagtggaag tcgaaccatc tgtaga
//
LOCUS       DAZKFZ010000456         1123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000456 DAZKFZ010000000
VERSION     DAZKFZ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..565
                     /gene="espM2"
                     /locus_tag="ACHOQW_005068"
     CDS             <1..565
                     /gene="espM2"
                     /locus_tag="ACHOQW_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFP9575932.1"
                     /translation="FSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTISFSVRKFSG
                     NILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEERQRVFFLVS
                     QENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTIANLLADKFY
                     QKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            799..951
                     /locus_tag="ACHOQW_005069"
                     /pseudo
     CDS             799..951
                     /locus_tag="ACHOQW_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          357 a          208 c          265 g          293 t
ORIGIN      
        1 tttcagctct tttggtataa gttgccatag agagaactcc ttcaggaact ctttccgagg
       61 gaagaatgat gaggtcgtaa aatgttcaat tggagaacgg actattagct tttctgttcg
      121 caaatttagc ggcaatatac tggaaacggt gagaaggcag agtactaaag atattgatga
      181 gtggataaaa gatgaacgga tagtatatcc ctcaagggtg atcaaccagg agattgataa
      241 ttactgtttt caaaaaaatg caaaaatttc cactgaagag aggcaaagag tcttttttct
      301 tgtgagccag gagaatcaac taacccttga tgttaaggca gcacaaagtt ctattaatca
      361 tgtaataatg gggagtgctt cttttggcaa gaaaatggat gctctttgtg atggtatgag
      421 ccgggatgta aaaaatcgta catcagatac catagcaaac ttgcttgcgg acaagtttta
      481 ccagaaacat atcgattcgg atattgatat tgtaaaacta cgaaatgata tcccagatta
      541 tttgatgcgt gctatacagg gatgaggcta gaggagagga ctgcttcagc atcatcggct
      601 tcggaccagt aaaagagacg cagaaaatgc acaacggcac cacacgccat gcatggattc
      661 agattactca tggctcacca gtacagctat aaatcgtaaa gaaaactaca gtacgttata
      721 cacagaaacg taccgtgatt cattatttat tgtacaggtt catcgcagcg acaatatttt
      781 gtgatgtttg cgtggtgcgc cccacttcag gatgctgcag atctggaaat tgcaacggag
      841 gaagaaacct cattgctgga agcctagaaa aagtatcggg tgttgctgaa ccgtgttgat
      901 acatcaactg cacctgatat tgagtggccg gaagaaccag acacaatgta agcgaaaaag
      961 aaaaaccgca gacacgacgt atgcaggacg tgctgcggtt ggctggtgaa ctttcgatag
     1021 tgcgagtatt gaatgatttc cagccgttac cgattttacg tgttaattag tgaacaaacc
     1081 actcgtcagc agacttccag gtatctttca gagtctcctg aac
//
LOCUS       DAZKFZ010000457         1122 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000457 DAZKFZ010000000
VERSION     DAZKFZ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..82)
                     /locus_tag="ACHOQW_005070"
     CDS             complement(<1..82)
                     /locus_tag="ACHOQW_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9575933.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVG"
     gene            complement(307..861)
                     /locus_tag="ACHOQW_005071"
     CDS             complement(307..861)
                     /locus_tag="ACHOQW_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9575934.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(924..>1122)
                     /locus_tag="ACHOQW_005072"
     CDS             complement(924..>1122)
                     /locus_tag="ACHOQW_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9575935.1"
                     /translation="VALIVRQQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDA
                     LTHAGLLIDDEQFDEINIVRG"
BASE COUNT          273 a          317 c          209 g          323 t
ORIGIN      
        1 ccccgactgt cgccggttcg gtaacgctta attcattaaa caccactctg gcggtttctg
       61 tcatatcctg ctgttttagc atgtcttttt cccttttccg gttaacgtga cataccaata
      121 actcttgtcg aaaaagccag caagttgaaa gaccggtatt agcaaccacc agcgcgttta
      181 acgccccgtg ccgtttttca gtcataaaaa aacccgcaaa aagcgggctc tttcaaatgt
      241 ccatgtctgc tattcgcctc gcggtacagc tttgcgaagc ttaccggaat tgaagcagtt
      301 tttacgtcaa aaagcaataa cttttttctc tataccaaaa gccataacca ttggtttgta
      361 caaaataaat tctgccacct ttagccaatg ctcaatgcgt ctttcacagg ttcttaaact
      421 ccattccgga tgtgcatcat tcagcaattc agccattttg cgcttagtca tcccccgccc
      481 cacataacgc tgactcagaa cattgagcag cccgggataa cctgccagta cttcaccaat
      541 aaccctatcg attattaacg cctctgaatc ggtacaatgt gccagccagc ttttttgatt
      601 gccgttgatc atatcccgca aaaaagcctc aagttcaggt ttgtccagac ccgctttttt
      661 catcctccgg agcgcctcgt taattgccgt ttttgtcagc tttttagagg tcagtaattg
      721 gttgaacata tttcccgtct taccgtcgcc aatatacgac caacgccccc acatacgcag
      781 tttcccctgg atccagacac tttccagcgt tttcaggcgt aaatgctcac cgcttttgcc
      841 tgtaatttcc gggtatatca tatttatgct cactcacttt caattttgta aatcttcacg
      901 cccagccgcc caccagaaac gagctagccg cgcacaatat tgatttcatc aaactgctcg
      961 tcgtctatga gaagtccggc atgcgtcagc gcatccagtg gtgctttcag gatattgtcc
     1021 aggtcacgac ggcgcttatc cggtggctct gcaataatct ttatcgccag ccttccggac
     1081 aggtttaatt tcagccgctg ctggcgaaca ataagtgcca ca
//
LOCUS       DAZKFZ010000458         1122 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000458 DAZKFZ010000000
VERSION     DAZKFZ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.462"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..549
                     /locus_tag="ACHOQW_005073"
     CDS             <1..549
                     /locus_tag="ACHOQW_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9575936.1"
                     /translation="SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVI
                     HIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGAS
                     MILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRI
                     SQDISTRDEGGDGKVVVIGRQA"
     gene            809..>1122
                     /locus_tag="ACHOQW_005074"
     CDS             809..>1122
                     /locus_tag="ACHOQW_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575937.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TIT"
BASE COUNT          272 a          269 c          373 g          208 t
ORIGIN      
        1 tcgatgcaga tgccgggatt ccgccgtcag atgaacgaag gctggtacca gatacgtatt
       61 gccggtgatg acacggcacc ggaggcggtg tacgcccgtc ttcacgaaca gctgggtgag
      121 ggaacggtca tccacattgt gccgcgactg gccggggccg gaaagggtgg actgcagatt
      181 gtgctggggg cggcggccat cgtggggtcg ttcttcacgg ccggggcatc aatggcgtta
      241 tggggttcag ccctggcagc cggtggtttt tctgccacca cgatgctgtt ttcactgggg
      301 gccagcatga tactgggcgg tgtggcccag atgctggcac cgaaggcaaa aacaccggat
      361 taccgcgcaa cggataacgg cagacagaac acgtactttt cctcactgga caacatgatt
      421 gctcagggga acccgatgcc ggtgccttac ggtgaaatgc tggttggctc acggcgaatc
      481 tcccaggaca tcagtacccg tgatgaaggc ggtgacggga aggtggtggt tatcgggcgg
      541 caggcataaa agcgaaaaaa tcccgcagtg accgaaggct gcgggaacag aaaatgaaga
      601 ttaaccacag ggagttttgt ttttattggc ccgaaaaaac tgtaacgccc gggaatgata
      661 tctgccacgg gggcgtacag aaaatgtgaa gaaattcaga aattttattc cgtcatgaca
      721 caggcaccct ccggggtgcc tgtcgttttt ggggcataaa cagattcaga catcagacat
      781 cagacatcag acaggagagg gggaccgagt gggtaaaggt ggcggcaggg cacacacgcc
      841 ggttgaggca aaggacaatc ttaagtccac gcagatgatg agcgtgattg atgccattgg
      901 tgaagggccg attgaaggtc cggtgaaggg gctgcagagt atcctggtga acaaaacccc
      961 gctgacggac acggacggta atcctgtgat acatggtgtg accgcggtct ggcgcgccgg
     1021 ggagcaggag cagacaccac ctgaaggctt tgagtcctcc ggggcggaaa ccgcactggg
     1081 cgtggaagtg acgaaggcaa agccggtgac gcgcaccatt ac
//
LOCUS       DAZKFZ010000459         1117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000459 DAZKFZ010000000
VERSION     DAZKFZ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.463"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..678
                     /locus_tag="ACHOQW_005075"
     CDS             <1..678
                     /locus_tag="ACHOQW_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9575938.1"
                     /translation="ATGITTLLSYLASESEGSLKVQGWSASGGRAEVVSDAEGTGGKA
                     VKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQYVMAFYWPVSSLP
                     QGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGTFGAFDNEWHTLAFR
                     FAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRNATYPVLIDSIAVEV
                     NNADAAA"
     gene            815..994
                     /locus_tag="ACHOQW_005076"
     CDS             815..994
                     /locus_tag="ACHOQW_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9575939.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            1035..>1117
                     /locus_tag="ACHOQW_005077"
     CDS             1035..>1117
                     /locus_tag="ACHOQW_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9575940.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALK"
BASE COUNT          291 a          265 c          324 g          237 t
ORIGIN      
        1 gccacaggca tcacaacact gctctcttac cttgccagcg agtcagaggg aagcctgaaa
       61 gtacagggat ggtcagccag tggcggcagg gcagaagtgg tcagcgatgc ggagggaacc
      121 ggaggtaagg cagtgaagct gaccaaggaa gccggtaaaa gcagctgggt gctggagtac
      181 gccgcgggca acggtgcggc tctgttacag aaaggggggc agattcgctg ccgctttaag
      241 gtttcgggag cgctggctgc gaaccagtat gttatggcgt tttactggcc ggtctcttca
      301 ctgccacagg gcgttgccct gaccggagac ggggggaata acctgctggc agcgttctac
      361 atccagacag atgcaaaaga cctgaatgtg atgtaccaca atgcgaaagt ggcgacaaac
      421 aacctgaaac tgggaacctt tggcgcattt gataacgaat ggcatacgct ggctttccgc
      481 tttgccggga ataacagcct tcaggtgacg ccggttattg atggtcagga tggcacaccg
      541 ttcacgctga cgcagtcacc ggtcagtgca tttgcggcgg ataaactgca tgtgacagac
      601 attaccagga atgcgactta cccggtgctg attgacagca ttgcggtgga agtgaacaac
      661 gcggatgccg cggcatgata aaaaaagccg ccagttgcct gagacctctg gcggtgtgag
      721 attcatggag aatcaaggaa agatactacc actttcgtca tcggcatttt taaatgaaaa
      781 ctgtttactt agtcaaccat aacggtaaga aattatgaca tttctgaacc agttaatgct
      841 gtacttctgt acggtggtct gtgtgctgta tctcctttcg ggtgggtaca gggccatgcg
      901 tgacttctgg cgcagacaga ttgacaaaag ggccgctgag aaaatcagcg ccagtcagtc
      961 agccggaagc aaacccgaag agccgctcat ttagcggcaa ctttcttaat cacatctttc
     1021 gacgagaaaa tcccatgtca gaaattacat ccctggtcac tgctgaagca gtgaaggaag
     1081 tcctgcgctc tgaagaagtc cggagcgcac tgaaaca
//
LOCUS       DAZKFZ010000460         1105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000460 DAZKFZ010000000
VERSION     DAZKFZ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.464"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            126..>1105
                     /locus_tag="ACHOQW_005078"
     rRNA            126..>1105
                     /locus_tag="ACHOQW_005078"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          295 a          240 c          345 g          225 t
ORIGIN      
        1 agacaatctg tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa
       61 taccaagtct caagagtgaa cacgtaattc attacgaagt ttaattcttt gagcatcaaa
      121 cttttaaatt gaagagtttg atcatggctc agattgaacg ctggcggcag gcctaacaca
      181 tgcaagtcga acggtaacag gaagaagctt gcttctttgc tgacgagtgg cggacgggtg
      241 agtaatgtct gggaaactgc ctgatggagg gggataacta ctggaaacgg tagctaatac
      301 cgcataacgt cgcaagacca aagaggggga ccttcgggcc tcttgccatc ggatgtgccc
      361 agatgggatt agctagtagg tggggtaacg gctcacctag gcgacgatcc ctagctggtc
      421 tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac gggaggcagc
      481 agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt gtatgaagaa
      541 ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta atacctttgc
      601 tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc cgcggtaata
      661 cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg cggtttgtta
      721 agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg gcaagcttga
      781 gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag atctggagga
      841 ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg aaagcgtggg
      901 gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga cttggaggtt
      961 gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg
     1021 ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg agcatgtggt
     1081 ttaattcgat gcaacgcgaa gaacc
//
LOCUS       DAZKFZ010000461         1101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000461 DAZKFZ010000000
VERSION     DAZKFZ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.465"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(58..429)
                     /locus_tag="ACHOQW_005079"
     CDS             complement(58..429)
                     /locus_tag="ACHOQW_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9575941.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(419..790)
                     /locus_tag="ACHOQW_005080"
     CDS             complement(419..790)
                     /locus_tag="ACHOQW_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9575942.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(803..>1101)
                     /locus_tag="ACHOQW_005081"
     CDS             complement(803..>1101)
                     /locus_tag="ACHOQW_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9575943.1"
                     /translation="SFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQG
                     GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT          304 a          306 c          209 g          282 t
ORIGIN      
        1 aaaaataaac atgttaatta taaacacaga aaacataacc ctcatcacta tatatcccta
       61 ccgcatatcc atatctaacc ggacattcag agccataagc attccttcaa taataccttc
      121 cgctttataa agccttttac caataagccc atcagaacat ctatgcttac gtgcaagagc
      181 cataaatgtc attccaccta cgtaataatc caccaacaaa tcgtgcaaat actgattatt
      241 cttgtttaat cgtgccatac aaccacaaat tatcatggcg tcatcatcag aacacttagg
      301 acgtgatttc actttagtcg ggattaatcc tttaaaacca gcagctatcg agggccatgt
      361 tacatcttca tgattatttg ctgcccatgc cccccatcgc tcaagaacca tctggatatc
      421 acgcgccatc gttaccacct ctgatttcgt aaatcttcac gcccagccgt ccacctggca
      481 caggctgacc gcgcacaata ttgatttcat caaactgctc gtcatcaatg agcacttccg
      541 catgcgtcag cgcatccagc ggtgctttca gaatgttgtc caggtcacgg cggcgcttat
      601 ccggtggttc tgcaataatt tttattgcca gccgtccgga caggcttaat ttcagccgct
      661 gctggcgaac aataagcgcc acagcccggc gataacgctc accggctttt gatacaaaat
      721 atgtgctgcc acgacgtcgc cagtaggtgt tcaccgtcgg cgggtaaggc aaaacaaact
      781 ctatacgcat cagtaacctc ttttacccga gcacgccggt tgcaaaagcg tgatcaagaa
      841 aacgaaaaat taaatcaacc tgggaaccat gcttttcttc gaacgccagc ggatccgcat
      901 gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa aatatcgtgg gattttgttc
      961 ccattccacc ctgaccatga ccaatcagat gatgaggatc gtccgctggc ttaccacaac
     1021 acgcacacgg ctgtgtcttt acccagcgtg tgtatttctc atttacccag cggcgacgtt
     1081 taggtcgttt catgaaggat t
//
LOCUS       DAZKFZ010000462         1100 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000462 DAZKFZ010000000
VERSION     DAZKFZ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.466"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1100
                     /locus_tag="ACHOQW_005082"
     CDS             <1..>1100
                     /locus_tag="ACHOQW_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9575944.1"
                     /translation="RRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGR
                     YITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTY
                     EDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRK
                     VKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCS"
BASE COUNT          292 a          237 c          363 g          208 t
ORIGIN      
        1 cgccggacgg ggaaacgggt gtggcgtcgc gggcgtgacc tgacgggatg gatgtcgctg
       61 tcgcggaaac cggaggtgac gtggtacggg tgggacggcg accggctgac gacggtacag
      121 accgacacca cgcgtatcca gacggtatac gagccgggaa gcttcgcgcc gctcatccgc
      181 atcgaaacag acaacggtga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
      241 cagcaggaag ggagcgagga cgggcacggt gtggtatttc cggctgaact ggtgcggctg
      301 ctggacagac tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
      361 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
      421 ccggcgagaa aagttcatct ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
      481 agcgaagacg gcaatacggc gtggagcggg gagtatgatg aatggggcaa ccagctgaat
      541 gaggagaacc cgcatcacct gcaccagccg taccggctgc cggggcagca gtatgataag
      601 gagtcggggc tgtactacaa ccggaaccgg tactacgatc cgttgcaggg gcggtatatc
      661 actcaggacc cgatagggct ggagggggga tggagtctgt atgcgtatcc gctgaatccg
      721 gtgaatggta ttgatccatt agggttaagt cccgcagatg tagcgctaat aagaagaaaa
      781 gatcaactaa accatcaaag agcatgggat atattatctg atacttatga agatatgaag
      841 agattaaatt taggtgggac tgatcaattt ttccattgta tggcattttg tcgagtgtct
      901 aaattaaatg acgctggtgt tagccgatcg gcgaaagggc tgggttatga aaaagagatt
      961 agagattacg ggttaaatct gttcggtatg tacggcagaa aagtaaagct atcccattct
     1021 gaaatgattg aagataataa aaaagacttg gctgtaaatg accatgggtt gacatgtcca
     1081 tcaacaacag attgctcaga
//
LOCUS       DAZKFZ010000463         1093 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000463 DAZKFZ010000000
VERSION     DAZKFZ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.467"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..102)
                     /gene="rrf"
                     /locus_tag="ACHOQW_005083"
     rRNA            complement(<1..102)
                     /gene="rrf"
                     /locus_tag="ACHOQW_005083"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(197..>1093)
                     /locus_tag="ACHOQW_005084"
     rRNA            complement(197..>1093)
                     /locus_tag="ACHOQW_005084"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          240 a          346 c          245 g          262 t
ORIGIN      
        1 tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt
       61 cggcatgggg tcaggtggga ccaccgcgct aaggccgcca ggcaaattct gttttatcag
      121 accgcttctg cgttctgatt taatctgtat caggccgaaa atcttctctc atccgccaaa
      181 acaccttcgg cgttgtaagg ttaagcctca cggttcatta gtaccggtta gctcaacgca
      241 tcgctgcgct tacacacccg gcctatcaac gtcgtcgtct tcaacgttcc ttcaggaccc
      301 ttaaagggtc agggagaact catctcgggg caagtttcgt gcttagatgc tttcagcact
      361 tatctcttcc gcatttagct accgggcagt gccattggca tgacaacccg aacaccagtg
      421 atgcgtccac tccggtcctc tcgtactagg agcagccccc ctcagttctc cagcgcccac
      481 ggcagatagg gaccgaactg tctcacgacg ttctaaaccc agctcgcgta ccactttaaa
      541 tggcgaacag ccataccctt gggacctact tcagccccag gatgtgatga gccgacatcg
      601 aggtgccaaa caccgccgtc gatatgaact cttgggcggt atcagcctgt tatccccgga
      661 gtacctttta tccgttgagc gatggccctt ccattcagaa ccaccggatc actatgacct
      721 gctttcgcac ctgctcgcgc cgtcacgctc gcagtcaagc tggcttatgc cattgcacta
      781 acctcctgat gtccgaccag gattagccaa ccttcgtgct cctccgttac tctttaggag
      841 gagaccgccc cagtcaaact acccaccaga cactgtccgc aacccggatc acgggtccac
      901 gttagaacat caaacattaa agggtggtat ttcaaggtcg gctccatgca gactggcgtc
      961 cacacttcaa agcctcccac ctatcctaca catcaaggct caatgttcag tgtcaagcta
     1021 tagtaaaggt tcacggggtc tttccgtctt gccgcgggta cactgcatct tcacagcgag
     1081 ttcaatttca ctg
//
LOCUS       DAZKFZ010000464         1091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000464 DAZKFZ010000000
VERSION     DAZKFZ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.468"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1091
                     /locus_tag="ACHOQW_005085"
     CDS             <1..>1091
                     /locus_tag="ACHOQW_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9575945.1"
                     /translation="VAVATAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKP
                     GIPRPALDSWDAEYMQENRWDEASASFVPVETESDPMNVTFDNLAPEVQNAVMVKFDT
                     CENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKP
                     GAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARARANQQHTENSTGKINPVIAAI
                     HREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNGREDWKRISASMRK
                     QPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGTNQSATDTLS
                     SPVPETDAVETAIPDNEKTECKVEVEPSV"
BASE COUNT          316 a          271 c          272 g          232 t
ORIGIN      
        1 gtcgctgtag ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac
       61 tggcaattcc tggaacatta tccggctgct caggactgcg cttataaatt tcttgtttgc
      121 gaggataaac ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag
      181 gaaaaccgct gggatgaggc gtctgcttcc tttgtcccgg tcgagactga atccgatccg
      241 atgaacgtca cttttgacaa cctggcccct gaagtacaga acgctgtcat ggttaagttc
      301 gacacatgtg aaaacatcac tgttgatatg gtgattagcg cacaggaatt gttgcaggaa
      361 gacatggcaa cattcgacgg ccatatcgtt gaagcgttga tgaaaatgcc agaagttaac
      421 gctatgtatc cggagcttaa gctgcatgcc atcgggtggg ttaagcataa atgtaagcct
      481 ggtgccaaat ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt
      541 gaacgcaagg aagccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagccaat
      601 caacagcaca ctgaaaattc aacaggaaaa atcaacccgg tcattgctgc cattcatcgc
      661 gaatacaagc agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt
      721 gatgtggatg ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc
      781 gacaacggcc gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc
      841 cttcgctacg accgccagac tatttttggc cttgtccgtg aacgtccgat cgacattcac
      901 aaagaccctg tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt
      961 gaagatgaag gaacaaatca gagcgcaact gatactctct cgtcgccagt accagaaact
     1021 gatgcagtgg aaacggcaat tccagacaac gaaaaaaccg aatgcaaagt ggaagtcgaa
     1081 ccatctgtag a
//
LOCUS       DAZKFZ010000465         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000465 DAZKFZ010000000
VERSION     DAZKFZ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.469"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            72..>1073
                     /locus_tag="ACHOQW_005086"
     CDS             72..>1073
                     /locus_tag="ACHOQW_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9575946.1"
                     /translation="MTFKHYDVVREASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRAPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPEPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSADGVPWICGDTTYFWKQKNEATYQTVYGSYKNKTEKNIHFVPFMTDENG
                     "
BASE COUNT          254 a          246 c          337 g          236 t
ORIGIN      
        1 tatagcgtgt tttttatgtg ttacatgatt gtgtgttccg gataattact ttgctggcgg
       61 caggagaggt tatgacattt aaacactacg atgtggtcag ggaggcatcg ccgtcagacc
      121 ttgctgaacg actgacacaa aaactgaagg agggctggca gccatttggc agtccggtgg
      181 ccatcacgcc ttacaccctg atgcaggcca ttgcggcgga aggtgatgtc accacaccag
      241 tggcggtgac cggtaatgag ggtaaggcgg tggctgtcag tgccaccaga gccccggagt
      301 attactttgt tgtggttctg gcagggcagt caaacggcat gtcgtatggt gaaggtcttc
      361 cgctgccgga gacatatgac cgtccggagc cgcgtattaa gcaactggcg cgtcgcagta
      421 cggtgacacc gggtggtgca gcatgcagat ataacgacat cattccggcg gaccattgtc
      481 tgcatgatgt gcaggacatg agccgtctta accatccgaa agcggacctg tcaaaggggc
      541 agtacggaac cgtggggcag gggctgcata tcgccaaaaa attgctgccg tttataccgg
      601 cgaatgcggg cattctgctg gttccgtgct gtcgtggtgg ttcagcgttc accaccggag
      661 ctgatggcac atacagtgac gcgagtggtg cttcggagaa ttcaacccgc tggggggtgg
      721 acaagccgct gtataaggac cttatcggtc gaacaaaagc agcactgaag aagaacccga
      781 aaaatgtgct gtttgccgtg gtgtggatgc agggggaatt tgattttggc ggtacgccgg
      841 caaatcacgc agcacagttt ggtgcgctgg ttgataaatt ccgtgcagac ctggcggata
      901 tggcaggtca gtgcgtcggt ggctctgctg acggtgttcc ctggatatgc ggggacacga
      961 cgtatttctg gaagcagaag aacgaagcca cctaccagac ggtgtacggc agctacaaaa
     1021 acaaaacgga aaaaaatatc catttcgtac cgttcatgac ggatgagaac ggg
//
LOCUS       DAZKFZ010000466         1063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000466 DAZKFZ010000000
VERSION     DAZKFZ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.470"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1063
                     /gene="espG"
                     /locus_tag="ACHOQW_005087"
     CDS             <1..>1063
                     /gene="espG"
                     /locus_tag="ACHOQW_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFP9575947.1"
                     /translation="VNSGFKKSWDEMSCAEKLFKVLSFGLWNPTYSRSERQSFQELLT
                     VLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEIRTANNEKITVLLESNEQNR
                     LLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTSKLSTTLIPHNAQTDPLSGP
                     TPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALGLKDTHSSPTRNVIDHGISR
                     HDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQSFNVPALTLTHERISKASE
                     YNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAPEDRPNEMGMLTNRTSYEVP
                     QGVKCIIDEMVSALQPRYAA"
BASE COUNT          331 a          230 c          215 g          287 t
ORIGIN      
        1 agttaattct gggtttaaaa aaagctggga tgagatgtca tgcgctgaaa agttatttaa
       61 agtacttagt tttggtttat ggaatccaac gtacagtcgt agtgaaagac aatcatttca
      121 agagttgtta accgttttag agcctgtata tccacttccc aatgaattag gcagagtatc
      181 tgctcgtttt tcagatggtt catccttaag aatttccgtc actaacagcg aacttgttga
      241 agccgagatt cgcacagcaa ataatgaaaa gattactgtg ctcctggagt caaacgaaca
      301 aaataggtta ttacaatctt tacccatcga tcgccacatg ccatacattc aggttcatcg
      361 tgccttatct gagatggacc tgactgatac tacctcaatg cgcaatctac ttggttttac
      421 gtcaaaacta tcaacaacct tgattcctca taatgctcaa acagatccgc tttccgggcc
      481 tacaccattc agctctatct ttatggatac atgtcgagga ctcggcaatg caaagctttc
      541 actcaatggt gttgatatac ctgcaaatgc acaaaaattg cttcgcgatg cactaggact
      601 taaagacaca cattcatcac caacccggaa tgttatagat catggtattt ctcgccatga
      661 tgcagagcaa atagcaagag aaagcagcgg cagtgataaa cagaaagctg aagttgtgga
      721 atttttatgc catccagaag cagcaacggc catatgctcg gctttctatc aatctttcaa
      781 tgtgccagcc ttaacgttga cacatgaaag gatctctaaa gccagtgaat acaatgcgga
      841 aagatcatta gatacaccta acgcttgcat taacatcagt atctctcaat catcagatgg
      901 aaacatttat gttaccagcc atactggggt tctgataatg gcgccagaag accgccccaa
      961 cgagatgggc atgttgacga acaggacttc ttatgaagtg ccgcaaggtg tgaaatgtat
     1021 aatcgatgaa atggtaagtg cgctacaacc aaggtatgcc gca
//
LOCUS       DAZKFZ010000467         1054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000467 DAZKFZ010000000
VERSION     DAZKFZ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.471"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..327
                     /locus_tag="ACHOQW_005088"
     CDS             <1..327
                     /locus_tag="ACHOQW_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9575948.1"
                     /translation="AGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAK
                     VPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKV
                     VVIGRG"
     gene            568..>1054
                     /locus_tag="ACHOQW_005089"
     CDS             568..>1054
                     /locus_tag="ACHOQW_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575949.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNHSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQ"
BASE COUNT          267 a          242 c          335 g          210 t
ORIGIN      
        1 gccggggcat caatggcgtt atggggttca gccctggcag ccggtggttt ttctgccacc
       61 acgatgctgt tttcactggg ggccagcatg atactgggtg gtgtggctca gatgctggcc
      121 ccgaaggcta aagtaccgga gtacaaaagc acggataacg gcagacagaa cacgtacttt
      181 tcctcgctgg ataacatgat tgcacagggg aacccgatgc cggtgcctta cggtgaaatg
      241 ctggttggct cacggcgaat ctcccaggac atcagtaccc gtgatgaagg cggagacggg
      301 aaagtggtgg ttatcgggcg gggatgaaaa taaaaaaatc ccgcagagtt agcggagctg
      361 cgggagagaa cgatgaagat taacgttatg gagttatttt tcaggcatca aaaaagtaat
      421 gcagcgtcat tattgcggct acaggcaatt gccggaaatg tgaagagttt cagaaatttt
      481 attccgtcat gacacaggca ccctccgggg tgcctgttgt tttctggcat aaacagattc
      541 agacatcaga caggagaggg ggacagagtg ggtaaagggg gcggcaaggc gcacacgccg
      601 gttgaggcaa aggacaatct taagtccacg cagatgatga gcgtgattga tgcgattggt
      661 gaagggccga ttgaaggtcc ggtgaagggg ctgcagagta tcctggtgaa caaaaccccg
      721 ctgacggaca cggacggtaa tcctgtgata catggtgtga cagcggtctg gcgcgccggg
      781 gagcaggagc agacaccacc tgaaggcttt gagtcctccg gagctgaaac cgcactgggc
      841 gtggaagtga cgaaggcaaa gccggtgacg cgcaccatta cgtccgcgaa cattgaccgc
      901 ctgcgggtca ccttcggggt gcagtcactg ttggagacca cctcaaaggg cgaccgtaat
      961 cactcttctg tccgactgct gattcagttg cagcgtaacg gtaactgggt gacggaaaag
     1021 gatgtcacca ttaacggcaa gaccacctcg cagt
//
LOCUS       DAZKFZ010000468         1049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000468 DAZKFZ010000000
VERSION     DAZKFZ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.472"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..482)
                     /locus_tag="ACHOQW_005090"
     CDS             complement(<1..482)
                     /locus_tag="ACHOQW_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9575950.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNMAAT
                     G"
     gene            complement(493..>1049)
                     /locus_tag="ACHOQW_005091"
     CDS             complement(493..>1049)
                     /locus_tag="ACHOQW_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9575951.1"
                     /translation="VTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA
                     EDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSELTAMTASF
                     VLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKC
                     MRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          218 a          374 c          265 g          192 t
ORIGIN      
        1 ccagtcgccg ccatattgtc caggtaaagg ttctgaccgt tgcgccacca gtcatcctca
       61 cgcacaaaat ccggcagcgt tatccccgcc agatggtatg catcccggaa cagggtgtaa
      121 cagtccgtca ccccgtgctc aaaacgacgc ccggtcaggt gcggcacaca gcggaatttg
      181 tgaatttccc cccggcagac cagccaccag ggcagggcac tctttatctg cagccgccgg
      241 tcggcctcgc tcagccaggg cagaccacca gggtggctgt gaaccagcgc cacaatctcc
      301 ccctgcatct ctgcctgcag ccagtcttcc ggcgcaatac gaaaatacgc ctccggctct
      361 gcggagatat tcacgcaggg ctggtaccgt tcgccctccg gggtgcctat cacgaagccg
      421 cacgactccg caggcgcaca ccgccgggca tgcgccagaa tcgctgattc agtctgtgtc
      481 ataaaccggg atttactgcg aaagtttatt aatggaaagg aaaccgccaa aattagccac
      541 catgccgcgc atctcacacc cgcgcatgca cttgctgcat ctgtccttac ggatatccgt
      601 ggtggggttg tcgaactcat ccgccaccgc aggaccgtta tacccgcatt catctccccg
      661 gtaatcccac atacaggtgt tcgccagcat gatgcgaccg ggaaacagcg ctccgtccgt
      721 ctccgtcggt gtggccagca caaacgaggc cgtcatggcc gtcagctctg acatctgctc
      781 caccacccac cggtcagtca gctcctgctc cgggtcggcc tccggattgc ctgccacaaa
      841 gttcaccgca tccagaaaac gcgcatacac ccggcggcgg accaccgtgg cacccaccag
      901 gctctgcaaa tcctccgcca tcccggtgac aagaccgaac agattggaca ccgtcagcga
      961 cgggcgggca ctgctgccct ttccgttcat ctcaaagccg ctgccctcaa tcgggtacgc
     1021 ctgatattca cgcccctgcc aggtcaccg
//
LOCUS       DAZKFZ010000469         1046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000469 DAZKFZ010000000
VERSION     DAZKFZ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.473"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..305)
                     /locus_tag="ACHOQW_005092"
     CDS             complement(<1..305)
                     /locus_tag="ACHOQW_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFP9575952.1"
                     /translation="MNNTLLPSINIPCTLFETISLFDNYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     "
     gene            492..638
                     /locus_tag="ACHOQW_005093"
                     /pseudo
     CDS             492..638
                     /locus_tag="ACHOQW_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(663..>1046)
                     /locus_tag="ACHOQW_005094"
     CDS             complement(663..>1046)
                     /locus_tag="ACHOQW_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575953.1"
                     /translation="FKSSIRNIFYPSKLERLSRFQFIIYTLTSWSILITLNDINLALS
                     FILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQL
                     NVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          342 a          174 c          195 g          335 t
ORIGIN      
        1 gaaaacattt ttgctagttc tttcatttct gcaaataaaa tatcagtgca ttcacgttgt
       61 gatatttttg ttccggaact tgaggcacta aaagcaacat taatagaacc tgggccaggg
      121 aaatggtatt taataagtct gtaaggatca acttttgctg agatatcgct taggccgagt
      181 gagagaaaat cttgttctac catatcacca tattgcatat catcagcaga ataattatca
      241 aataaactaa tagtttcgaa aagtgtacag ggaatattaa ttgatggaag taatgtgtta
      301 ttcatttaca ttttccttgg ttatatttaa gctcaatatc aatttgagaa caaatgctag
      361 ggtaaaaaaa tatttaaaaa agaagtacac ttcacatttt ttcattacat ctctgaagag
      421 aacgtcgtta atgattggtc ttgctcattg ttaataacaa aatattgaat ttaccgatta
      481 ggaaactttt tcctacgccg attgcctcca gctatggcat gaactttgag tttatgccag
      541 aactgaagtg gagtttcggc tacccaggcg cgattatctt tatgatcctc gcgggcctgg
      601 caccgtatct gtactttaag cgcaagaact ggctgtaact atacgatgtc gctgtgagta
      661 agttagtgtg ctgacagcga cattttagca gagggaatcg cagataacat aattgttgtg
      721 aataatatcc atgccacgtt gagttggacg cttaatgact cacatctcac cccttcaatt
      781 gctgcagaga taatagaaaa aagtgtcaaa cccaatgaaa tcaatacaaa gtagattgcc
      841 ttacatccag tatctcttat tctttttgct ccaatattga tatttacata ggtattatta
      901 ataaccagaa taaaactcaa agccaggttg atatcattca aagttattaa tattgaccat
      961 gatgttaatg tatagattat gaactgaaag cgtgataaac gctcaagctt ggatgggtag
     1021 aatatatttc tgatggatga tttgaa
//
LOCUS       DAZKFZ010000470         1046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000470 DAZKFZ010000000
VERSION     DAZKFZ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.474"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(147..842)
                     /gene="aqpZ"
                     /locus_tag="ACHOQW_005095"
     CDS             complement(147..842)
                     /gene="aqpZ"
                     /locus_tag="ACHOQW_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP9575954.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLTAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT          323 a          284 c          236 g          203 t
ORIGIN      
        1 gtcctgtaag acgcgccctc agcccacatc catgtgggct gccccggcct tcggggaacg
       61 cttcagcgat tttgacgcca ccaatacccg tgcggtttgt aatgtcgaag ataaaacaac
      121 cagggcagat aaaatgccaa tgctaattaa ttacgctttt ccagcagagt ccggtaaatc
      181 agaccaccga taatgccacc gacaattggc accacccaga agaaccacag ttgttctaat
      241 gcccagccgc cctggaagat ggcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta
      301 gtcaccggaa tactgattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca
      361 aaacctgccg gagcgaattt gtcggttgca ccgtggatca ctaacaggaa gcccgcactc
      421 aataccagtt caactaccag cgcggaaagc atggaataac cgcctggtga atgctcgcca
      481 taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc actggcaatt
      541 aaatacagca gcgctgccgc aacaataccg ccgacaacct gggcaattac gtagccaacg
      601 acttcttttg ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa
      661 tgaccaccag aaatatgacc aacagcaaag gccatcgtca gaacggtcag accgaacgcc
      721 aacgccacgc cggcaaaacc aatgcctaat tccgggaagc ctgcggtcag tacagcacta
      781 ccacagccac caaaaacaag ccagaaagta ccaaaacatt cagctgctaa ttttctgaac
      841 atatccacct caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg
      901 taaaaaagtg acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg agtcattcac
      961 cagataaata aatccagtaa atttgattta gggcaacagc gggttgcccc atatagtcat
     1021 ttgtctgatt gacagtgtag tgcaag
//
LOCUS       DAZKFZ010000471         1042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000471 DAZKFZ010000000
VERSION     DAZKFZ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.475"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..137)
                     /locus_tag="ACHOQW_005096"
                     /pseudo
     CDS             complement(1..137)
                     /locus_tag="ACHOQW_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            287..955
                     /locus_tag="ACHOQW_005097"
     CDS             287..955
                     /locus_tag="ACHOQW_005097"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9575955.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          307 a          230 c          201 g          304 t
ORIGIN      
        1 gctactgatg tatcaacacg attcaacaat acccgatatt tattccatgc ctccagcaac
       61 gatctttctt cctccgttgc gatttccaga tctacagcat cctgcagtgg cgcaatatac
      121 tcactgaatt cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc
      181 tgtatcgcag cataccaggc tatttcaata tcgctatgct gcggcagcat ttaacccctt
      241 gtaattcatc gccataattg atttaattca caaataaaac tataacatgg tgaaattaat
      301 gaaaaaaaac acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga
      361 ctggtgggtt ttgggagtat caaatcgtct tgcatgggga tgtcctacaa aggaacacct
      421 tcttccacac tttctggaac atttaggtaa caaccatctg gatattggtg ttggaactgg
      481 gttttacctt actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc
      541 tagcctgaac gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattagcca
      601 tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa tttgattcca tttccatgtt
      661 ttaccttctt cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc
      721 ggcgcaggcc ttaactgacg atggaactct atacggagcc acaattcttg gcgatggagt
      781 tgtgcacaat agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc
      841 aaacacaaaa gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt
      901 taaaaccaag gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc
      961 aaccgcagca cgttcttgca tacgccgtgt ctgcggttta atttttactc tcaaatattg
     1021 tagttaagta caataatgat tc
//
LOCUS       DAZKFZ010000472         1034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000472 DAZKFZ010000000
VERSION     DAZKFZ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.476"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            134..>1034
                     /locus_tag="ACHOQW_005098"
     CDS             134..>1034
                     /locus_tag="ACHOQW_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFP9575956.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTS"
BASE COUNT          356 a          201 c          200 g          277 t
ORIGIN      
        1 aaaccgcaat aaaaaacgaa taaatcaatt gtctcacgca attataaaac atcatattga
       61 ttacgcacct tgcattacaa actcatgtat gtaaaatacg cgcaccattc aaaaaaaagg
      121 aagacaataa catatgaaaa aaagtgtcat cgctggcgtc tttattgctc tgtcatttac
      181 cacgtgttca gctatcgcga acagccttgc attatcatta gcaaatgatg atgcagggaa
      241 gtttcaacca atacttaatg atatttatgg caataaacat gaaaacagag atgattactc
      301 acaaggctta tttctgggat atagccacga tatctcagac tcgagccaat tatctctcca
      361 tattgcgcaa gatatttact ctccatcagg cagtaataaa agacacaaca cagctgtaac
      421 tggagacaga gcttttagtg catacactca cactggtatt gaatggaact cccttgcgaa
      481 tgactggatt cgctatcgat taggtactga cataggtgtt gttggccccg acgcaggcgg
      541 tcagaaagta caaaataaag ctcatgagat tattggggca gaaaaatatc atgcatggga
      601 tgatcaaata gagaatcgct acggttatac tgtaaaaggg atgctatcca tgacaccaag
      661 tatggatatt ttaggtgcta atgttggatt ataccctgaa gtttctgctg ttactggaaa
      721 cttatttcaa tatgtagcat atggcgcaac cattgccatt ggtaatgata aaaccttcaa
      781 ttcggataat ggctttggtc tgctggctcc ccgtggttta atgcatatgt ccgatacaag
      841 cggattcaaa tacaagattt ttgcaggtat ggaaagacga gatgtcaatc gcaactatac
      901 tctcgaagga aaaacaatac agacgaaaca aacaacagta tcgctaaaca aaactgttga
      961 tgaatatcaa gttggcgcaa caattgggta tgcacctgta gccttcacac tagcatttaa
     1021 taaagtaaca tcag
//
LOCUS       DAZKFZ010000473         1031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000473 DAZKFZ010000000
VERSION     DAZKFZ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.477"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..736
                     /locus_tag="ACHOQW_005099"
     CDS             <1..736
                     /locus_tag="ACHOQW_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9575957.1"
                     /translation="SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFP
                     SKTPKNGSRNPKEPKDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMS
                     DSWYPSRDFRRRAALWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVN
                     HVRAQVKPVSKGVNHAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGG
                     GLFEPLDPEERGRTFEALDCTDWRDD"
     gene            729..>1031
                     /locus_tag="ACHOQW_005100"
     CDS             729..>1031
                     /locus_tag="ACHOQW_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9575958.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLA"
BASE COUNT          279 a          250 c          302 g          200 t
ORIGIN      
        1 gtctttaaag gtgggtaaaa ttggtgctgt taataaaaac agtaccgaaa atggttatgt
       61 ttcagcacaa aacagaccca aaaacagaac tcttagctgc atggaaaata accaaagaca
      121 cccaaatttt ccatcaaaga cacccaaaaa cggatcacgg aacccaaagg aacccaaaga
      181 tctaaacccc acacataacg cacgcgagag tgctccgacc agtgagcagg aagttttgtc
      241 gttacaggca gccccccctg tattcctgta tggcctgagc gaacccatcg gaaaattccc
      301 gatgagcgat agctggtatc cgtcacggga ttttcgacga cgggctgcgt tgtgggggat
      361 ggctttgccg gagacagaat ttacacctgc tgaacttgcc gccttccggg actactgggc
      421 agcggagggg aaagtgttta cgcagattca gtgggagcag aaattcgccc gtcacgtaaa
      481 tcacgtcagg gcgcaggtta aaccagtcag caaaggggta aaccatgcag cagcaccagg
      541 tggcaccgca tcacgggcag ttcaggaaat tcgggcagca cgtgagcagt gggaacgtga
      601 aaacggattt atcagcgacg gaaacggcct ggaagctgtg ggaactcatg ggggtggttt
      661 attcgaaccg ctggatccag aagaacgggg ccgcaccttc gaagctctgg attgcacaga
      721 ttggcgcgat gactgagcag caaatccggc aggtctgccg ccagtgcatg gaccgctgcc
      781 gggcgggtga aacatggcct ccggacctgg ctgagtttgt ggcactgatt tcgaaaagcg
      841 gagccaatcc attcggtctg acggtggatg ctgtgatgga ggagtaccgc cgctggcgca
      901 atgagtcctg gcgatacgac ggaagtgata agtacccgtg gtctcagcct gtgctgtatc
      961 acatttgcct cgagatgcgt tcaaagggga ttgagcgcca gatgaccgaa ggggaattaa
     1021 aacggcttgc a
//
LOCUS       DAZKFZ010000474         1031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000474 DAZKFZ010000000
VERSION     DAZKFZ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.478"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..>1031
                     /locus_tag="ACHOQW_005101"
     CDS             2..>1031
                     /locus_tag="ACHOQW_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9575959.1"
                     /translation="MPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVTTNRS
                     ASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASS
                     RVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFIT
                     KKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLP
                     NTNYRNNSQGVWLGYNSENHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREK
                     VGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHD
                     KQYTQAVTL"
BASE COUNT          297 a          236 c          243 g          255 t
ORIGIN      
        1 tataccttgt cgaatttata tcctttccac tctgtcatta tgcatttctg ggatagtttc
       61 tactgcaacc gcaacttctt cagaaacaaa aatcagcaac gaagagacgc tcgtcgtgac
      121 cacgaatcgt tcggcaagca acctttggga aagcccggcg actatacagg ttattgacca
      181 acaaacattg cagaactcca ccaatgcctc catagccgat aatttgcagg acatccccgg
      241 agtagagata acagacaact ccttggcagg ccgtaaacaa atccgcattc gtggcgaagc
      301 atcctcccgt gttttaattc tcattgatgg tcaggaggta acttatcagc gcgccggaga
      361 taattatggt gtgggactgt tgatagatga gtctgcgctg gagcgtgttg aggtagtgaa
      421 aggtccatat tccgtactgt acggttcaca ggcaattggc ggtattgtta acttcatcac
      481 caaaaaggga ggtgacaaac ttgcatctgg agttgtgaaa gctgtttata attccgcaac
      541 agcaggctgg gaagaatcaa tcgcggtcca ggggagcatc ggtggatttg attatcgcat
      601 caacggtagt tattctgatc agggcaatcg tgatacgccg gatggacgtc tgccgaatac
      661 caactatcgt aacaatagtc agggtgtatg gttgggttat aactccgaaa accatcgttt
      721 tggcctctcg cttgatcgct acagactcgc gacacaaact tactatgagg atccagacgg
      781 aagttatgag gcatttagtg tcaaaatacc taaacttgaa cgagagaaag ttggggtatt
      841 ctatgacaca gacgtggacg gtgactatct aaaaaaaatt catttcgacg cgtatgagca
      901 gaccatccag cgccaatttg ccaacgaagt aaaaacgaca cagcctgttc ccagtccgat
      961 gattcaggct ctgaccgttc ataacaagac tgacacccat gataagcaat acactcaggc
     1021 ggtcacattg c
//
LOCUS       DAZKFZ010000475         1025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000475 DAZKFZ010000000
VERSION     DAZKFZ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.479"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..96)
                     /locus_tag="ACHOQW_005102"
     CDS             complement(<1..96)
                     /locus_tag="ACHOQW_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575960.1"
                     /translation="MVCLYNFGVRAAMDNSKHSDEAEKLLAELSAR"
     gene            complement(129..>1025)
                     /locus_tag="ACHOQW_005103"
     CDS             complement(129..>1025)
                     /locus_tag="ACHOQW_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9575961.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          238 a          291 c          202 g          294 t
ORIGIN      
        1 tctagcagaa agctccgcca ataatttttc cgcctcatca gaatgttttg agttatccat
       61 agcagccctc actccaaagt tataaagaca taccatgata atcaacaaaa aagtaaatac
      121 tttcattact atttgcttcc tgtaggtgta aacttatgaa agtcccctgg ttgtttctcg
      181 tctggcacca attcaaacac cccgttgtcc ttaccgccta ttgttatcct agctgtttgc
      241 cccataatag catagtttcc atccggccaa atccatatta tttcatcagg agcaatcact
      301 gtagcatcaa gttcgttagc tagtttctgt gcaaatgaat ttttgccttt tcctgtttcg
      361 caggaaaaca agactacagg cataccttca tgatatttag gattcgctct tatgtcgcta
      421 gctaattgtt ttattgtcag atcacttcct tttttgattc tgtcatcact catagatgta
      481 ggcgaaccat gacctccaac tgtaaaaccc gttggaaatt cccttacatt aagagaacct
      541 ttatatatag gatctgacgg agaataataa ttcaaatcaa gtgctaaccc caacggatct
      601 atatgttcaa taggattgag tgggtactga tacaggttcc atcccccctc cagccctatc
      661 gggtcctggg tgatatatct cccctgcaac ggatcatagt accggttgcg gttgtagtac
      721 agccccgact cctcatcata ctgctgcccc ggcagacgga gtgactgttg aaggtgctgc
      781 gcgctggttt cgcccagcag gtttccccac tcgtcatact ccccctgcca cgccgtttcc
      841 ccttccgggc tgatgagcgc cagcggcagt ccccggtggt cgcagtggta aagatgaagt
      901 ttcctctccg ggatgtattc cgcctccagc tgcgcggcca tctgctccgc cgtcagcccg
      961 cactgcgcaa gccactgctg gctttcttca ctcacgctgc cctgccgcag ctcccgctcc
     1021 agcct
//
LOCUS       DAZKFZ010000476         1021 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000476 DAZKFZ010000000
VERSION     DAZKFZ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.480"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..356
                     /locus_tag="ACHOQW_005104"
     CDS             <1..356
                     /locus_tag="ACHOQW_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFP9575962.1"
                     /translation="IQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRL
                     MRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKP
                     KLDLTNTDWIYGVDL"
     gene            353..>1021
                     /locus_tag="ACHOQW_005105"
     CDS             353..>1021
                     /locus_tag="ACHOQW_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9575963.1"
                     /translation="MKHIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIK"
BASE COUNT          272 a          270 c          292 g          187 t
ORIGIN      
        1 cgattcagag cggcagcaag tgggggacag cagaagacct gaccgccgca gagtggatgt
       61 ttgacatggt gaagaccatc gcgccatcag ccagaaaacc gaattttgca gggtgggcta
      121 acgatatccg cctgatgcgt gaacgtgacg gacgtaacca ccgcgacatg tgcgtgctgt
      181 tccgctgggc atgccaggac aacttctggt ccggtaacgt gctgagcccg gccaaactcc
      241 gcgataagtg gacccaactc gaaatcaacc gtaacaagca acaggcaggc gtgacagcca
      301 gcaaaccaaa actcgacctg acaaacacag actggattta cggggtggat ctatgaaaca
      361 catcgccgca cagatggtta actttgaccg tgagcagatg cgtcggatcg ccaacaacat
      421 gccggaacag tacgacgaaa agccgcaggt acagcaggta gcgcagatca tcaacggtgt
      481 gttcagccag ttactggcaa ctttcccggc gagcctggct aaccgtgacc agaacgaagt
      541 gaacgaaatc cgccgccagt gggttctggc tttccgggaa aacgggatca cctcgatgga
      601 acaggttaac gcaggaatgc gcgtagcccg tcggcagaat cgaccatttc ttccatcacc
      661 cgggcagttt gttgcatggt gccgggaaga agcatccgtt atcgccggac tgccaaacgt
      721 cagcgagctg gttgatatgg tttacgagta ttgccggaag cgaggcctgt atccggatgc
      781 agagtcttat ccgtggaaat cgaacgcgca ctactggctg gttaccaacc tgtaccagaa
      841 catgcgggcc aatgcgctga ctgacgcgga attacgacgc aaggctgccg atgaactgac
      901 ctgtatgaca gcgcgaatta accgtggtga ggcgatacct gaaccagtaa aacaacttcc
      961 tgtcatgggc ggcagacctc taaatcgtgt tcaggcgctg gcgaagatcg cagaaattaa
     1021 a
//
LOCUS       DAZKFZ010000477         1017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000477 DAZKFZ010000000
VERSION     DAZKFZ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.481"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..703)
                     /locus_tag="ACHOQW_005106"
     CDS             complement(<1..703)
                     /locus_tag="ACHOQW_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9575964.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFT"
     gene            complement(709..>1017)
                     /locus_tag="ACHOQW_005107"
     CDS             complement(709..>1017)
                     /locus_tag="ACHOQW_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9575965.1"
                     /translation="VISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCT
                     WTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ
                     "
BASE COUNT          224 a          323 c          269 g          201 t
ORIGIN      
        1 ccgtaaaggc agatgcgcgc catgcccggt gacgccagag ggagtgtgtg cgtcgctgcc
       61 atttgtcggt atacctctct cgtttgctca gttgttcagg aatatggtgc agcagctcgc
      121 catcaccaca gtaaatggcg gcatgattcg gcaccgatga accaaaacag cacagcagca
      181 catcgcccgg ttgtgctgat gacaacggca cctgatacag ccctgtggcc tccagattat
      241 ccagatagag attctgaccg tgacgccacc agtcatcctc gcgatgaaaa tccggcatct
      301 caatccccgc cagatgataa gcatcccgga acagcgtgta acagtccgtc accccgtgct
      361 caaagcgccg cccggtgaga tgcggcacac agcggaactt atgaatcgtc ccccggcaga
      421 ccagccacca cggcaaatca ctctgcacct gcagccgccg gtcggcctca ctcagccagg
      481 gcagaccacc ggggtggctg tggaccagcg ccacaatctc accctgcatt tctgcctgca
      541 gccagtcttc cggcgacata cggaaatacg cctccggctc accggagata ttcacgcagg
      601 ggaaatatct ttccccctcc ggcgtgctta ccacgaagcc gcacgactcc gctggcgcac
      661 atcgccgggc gtgcgccaga atcgctgatt ctgtctgtgt catgggattt actgcgaaag
      721 tttgttaatg gaaaggaagc cgccaaagtt gccgacgtta ttgcggaact tacaaccgct
      781 caggcatttg ctgcatttat ccttcgtgat atcggacgtt ggctggtcat attcatccgc
      841 gaccgccgga ccgtgataac cgcactcatc gccgcgatag gtccaggtgc aggtgttggc
      901 cagcatgata cgtcccggaa aaacagcgcc gtccgtttcc gtcggcgtgg acagtacaaa
      961 agaggcactc accgcgctca gttcgctgca ctgctcaatg cgccagcggc tgatcac
//
LOCUS       DAZKFZ010000478         1012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000478 DAZKFZ010000000
VERSION     DAZKFZ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.482"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>1012)
                     /locus_tag="ACHOQW_005108"
     CDS             complement(<1..>1012)
                     /locus_tag="ACHOQW_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9575966.1"
                     /translation="GITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHI
                     QENSTSFFRNGGRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYN
                     PTTFSPVDAQTVEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQT
                     LIESIELLLDEALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRE
                     NLPPLAGGDALYLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISET
                     EHDAVKAMFRGDTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLT
                     SLS"
BASE COUNT          202 a          318 c          238 g          254 t
ORIGIN      
        1 ccgacaggga agtcagtttc tcttcaaacg cttgtgcctg cgaggccatc ttcccctcaa
       61 atgtgcgctg taaatccgcc agcactgtgg aaaattcttc tcccagtgca cgaataatgg
      121 acagttcccg ttccgtcatt ttctcagtat cccccctgaa catcgctttc accgcatcat
      181 gctctgtttc actgattgcc ttattaccgt cagatgcgcc gtcaggcagc tgtgatgaaa
      241 ctgttttccc ggcagacgcg aacggatcct cacgggcatc acgacgggac agcgcctcca
      301 gactgtagtt ctgctgctga agatacagtg catcaccgcc ggccaggggc ggcaggttct
      361 cccgtttacg ggcctcattg ggcgtgagaa gcgtattttt caccgcatcc cccagcgttt
      421 tcatgcgccg ctcactgtcc attctcagca gcgtggtgac atcaaattct gtactctcgt
      481 tttcccccgt ttccagcgcc tcatccagta acagttcaat ggactcaatc agcgtctgca
      541 ggcactggga ataatactgc tgctccagcg cctccacgtt gtcactggaa ggcggttgtc
      601 ccacgccaat cttgtaggcc gggacacgga acaccgaaca gacaatttca gcggtcatct
      661 tcagttgttc caccgtctgc gcatccaccg gtgaaaacgt cgtggggttg tattttgccc
      721 cgttgctcag aatggccgtt ttccccgcat tttcgcctgt atacccgctg tcccagttgc
      781 tcttcagttt tttcgcattt tcttccgtaa tactgccggg gatctcaatc accccggacg
      841 gcctgccgcc atttctgaaa aaagacgtcg aattttcctg aatatgatgc ccctgcgtgg
      901 ccgccagccc ggcggcatac accggcggca atcctataag cggatgaaaa aaacagttaa
      961 accggtcgtg gatcacttcc cgggcaggca ccgtcaccgc ctccgtgatc cc
//
LOCUS       DAZKFZ010000479         1004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000479 DAZKFZ010000000
VERSION     DAZKFZ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.483"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>1004
                     /locus_tag="ACHOQW_005109"
     CDS             <1..>1004
                     /locus_tag="ACHOQW_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9575967.1"
                     /translation="SLPGAPLARYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVA
                     HRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAG
                     LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRET
                     KFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRSGETVRYRYDDAYSELPATTT
                     DATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGR
                     LISVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYD
                     L"
BASE COUNT          228 a          283 c          353 g          140 t
ORIGIN      
        1 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
       61 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcatccgggc
      121 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc
      181 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
      241 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
      301 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
      361 tatgacgcgg caggaaggct aacggcgcag acggacgcgg cgggacggcg gacagagtat
      421 ggtctgaatg tggtatccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
      481 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      541 agccgccggg catatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
      601 acagtacgct accgctacga tgatgcgtac agtgagttac cggcgacgac aacagatgcg
      661 acgggcagca cccggcagat gacctggagc cgctacggtc agttgctggc gttcaccgac
      721 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggtccac
      781 cgcgaggaag gcatcagcct ttaccgccgc tatgacaacc gtggccggtt aatctcggtg
      841 aaagacgcac agggccatga aacgcggtat gagtacaacg ccgcaggcga cctgactgcc
      901 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
      961 agcaccacgc agggcgggct gacgcgcagt atggaatacg acct
//
LOCUS       DAZKFZ010000480          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000480 DAZKFZ010000000
VERSION     DAZKFZ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.484"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>988)
                     /gene="ehxB"
                     /locus_tag="ACHOQW_005110"
                     /gene_synonym="hlyB"
     CDS             complement(<1..>988)
                     /gene="ehxB"
                     /locus_tag="ACHOQW_005110"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP9575968.1"
                     /translation="HTTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQI
                     RNFLTGQALTSVLDLFFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRL
                     DDKFLRNAENQAFLVETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTG
                     QQGIQLIQKSVMVISLWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQ
                     QVGISVERLGDVLNTPVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYIS
                     KGDVIGIVGRSGSGKSTLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIG"
BASE COUNT          315 a          226 c          167 g          280 t
ORIGIN      
        1 ccaatctggc gtcgtaacca ttctggatct gcaagtgata aatcatgccc atcaattaaa
       61 atctgtccgg tctctggtat ataaaagcgc tgaagcagtt ttgttaatgt actttttcct
      121 gaaccagaac ggccaactat accgataaca tccccctttg atatgtataa attaatatta
      181 ttcaaaataa cattaccgtc agaagaatac cgaaatctga catttttaaa ttcgatatcc
      241 ccctgaattt ccggcagtat atttctgcct gactttttct ctaccggtgt atttaataca
      301 tcaccaaggc gctcaacaga aataccaacc tgctgaaaat cctgccaaag atgagccagt
      361 ctgataacag gagcaatgac ctgacctgca agcatattaa acgcgattaa ctgcccgatg
      421 cttatctctc ccgatatgac aagatgtgct cccatccata gactaattac cattacactt
      481 ttctgtatca gctgtatccc ctgctgccct gtcattgcaa ccagatttac cctgaaactg
      541 gaagcaacat acccggccag ttgtttatcc catgtagcaa tcatctgtgg tgatactgcc
      601 atggatttga ttgtatttat tgctgttacc gtttcgacaa gaaaagcttg attttctgca
      661 ttcctgagaa acttatcatc aagacgtcga cgtaataagg gtgatataaa caatgaccat
      721 ataacataac aaggtagtga caataatata accagtgtta attgagggct gtaataccac
      781 atgacacaaa aaaatattac agaaaaaaat agatcaagaa ctgatgtcaa cgcttgtccg
      841 gttaaaaaat tacgaatttg ctccagttcc cttactctgg caacggtctc tccgaccctg
      901 cgattttcaa aatatgaaac aggcaatgca agcaaatgtc tgaataactt agcacccagt
      961 tcgacgtcaa tacgacttgt agtatgag
//
LOCUS       DAZKFZ010000481          986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000481 DAZKFZ010000000
VERSION     DAZKFZ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.485"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            6..>986
                     /locus_tag="ACHOQW_005111"
     CDS             6..>986
                     /locus_tag="ACHOQW_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFP9575969.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPRE"
BASE COUNT          326 a          176 c          183 g          301 t
ORIGIN      
        1 agaaaatggt tgataacgta actgttagca gagtatgtat acaatctcct tctttcgtgc
       61 ctgatttgga tggagaaaaa aataaatctc aattattcgt tgacgatata gttgcgtatc
      121 ttaaaagtcc ttcagtttat tcacttgaaa aagaggggcc gttaaatcat tttgttaatc
      181 attgttctga agttgagtta ggtttctata gcgatggtgc atattcaatt cttgtctcca
      241 gatctaagca acaacctgaa ggtatgattt taaccgtaag cgatgcagac gcaatcaata
      301 tagtacatat ttccgtatct ccagtgctta taaaattcct ggatgatatt tttacttgcc
      361 ttcatacgta ccctgatgat gagagtttta caaaagagca gataaaagct aatagcaaat
      421 atgatattgt agattataat tgcctgttgc attttactgg aaaaccaaaa agtttaatag
      481 aatgtagaca ttttgctctg caatactgta tagattcaat gaatgagcat acagggaaag
      541 ttccattaaa ggcttactat tcatctccgg aagatataca aaaacatatt cctttcgagc
      601 ttgagcagca atttaacaat ctacaaaaaa atccaccacc cggtacatgc gtcgttgcca
      661 gtgataagtt tggggaggca ttatctgtct tttttcacag aatggaaaaa gagaagttaa
      721 cgcatatgac ggcaatcgtt caatctcaaa cacacgctat ggccgtccgc ttgaggatca
      781 aaaaaacgcc tgctggtgaa acagaatatg ttgtatcctt ttatgatcct aatgcaacca
      841 atactgcagt acgctataaa gcaaacaact gtgattcttt tgggtcattg caatcgttta
      901 taaatattca acaggcaaaa caaaaatggg taataacaga tatttgctcc gagtgtgtag
      961 gaataacccc ttatctccct cgggaa
//
LOCUS       DAZKFZ010000482          980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000482 DAZKFZ010000000
VERSION     DAZKFZ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.486"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..280)
                     /locus_tag="ACHOQW_005112"
     CDS             complement(<1..280)
                     /locus_tag="ACHOQW_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9575970.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMP"
     gene            complement(368..910)
                     /locus_tag="ACHOQW_005113"
     CDS             complement(368..910)
                     /locus_tag="ACHOQW_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575971.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
BASE COUNT          295 a          207 c          178 g          300 t
ORIGIN      
        1 taggcatgat tttaattttt atttgttgcc cagaattggc cgctactttc actactggtg
       61 gcgttaacat gaaaggaacc tgaatctttt ctggcggcag agacgtgtcg ccatcatcaa
      121 tccacgcctg catcagcgaa gagcggtttc cctgattcat cagttgtacc atgacctctt
      181 tctgctctgc cgggtaaatg atacgggtcc cgaaaataac cattccggca tgcacgggga
      241 atatcataga agagatgagt aaagataata accctttcat cttaatgcct cttatattta
      301 tgctggccgc catgaatatg gcggccattt taacttactg aaaaaacaag atgattattt
      361 taaatattta atcctgggca acacgcataa ccaatgaaga gttaactgta ccagatgcaa
      421 caccactgtc agtcaacttc agatattttg ccttaaacgg tattgcatat tgtccatcag
      481 cagttttata actttcaata ttaaatggtg cggaaatatc cagtacgtca ctgctatctc
      541 ccaaatagag atagtatcca actgtgtcat tactactgga tattgcctta tcattaccaa
      601 gaattttctc gttggtgatc aacgaaagtt ttacgccatc aatagtcttc ggacaagtaa
      661 ttttcagcat actgctggtt acaccaaccg cgccatcata agttgaatat ttcgctttac
      721 catcaaatac agtggtgggt acagtcggca tattaatact gactgaatca ccgccagcga
      781 caaatgtaca tggtgattct gcaatattac ctttaatggt taaagttccg gtatctactt
      841 cagcagcgaa aataccagca ctcataaata cagaagataa gacagcgaca gcaataattg
      901 aatgtttcat gatgcattcc ttaataagtt tattttgttt tgttaagagt ctgtatctct
      961 tggaaacaaa tttctcacat
//
LOCUS       DAZKFZ010000483          974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000483 DAZKFZ010000000
VERSION     DAZKFZ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.487"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>974
                     /locus_tag="ACHOQW_005114"
     CDS             <1..>974
                     /locus_tag="ACHOQW_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFP9575972.1"
                     /translation="HALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQME
                     ADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGG
                     SLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLL
                     ARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSGWVDACCRLLSPLEEALHGYVMTD
                     GKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQT
                     HLACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPS"
BASE COUNT          203 a          283 c          305 g          183 t
ORIGIN      
        1 ccacgccctg caggaaaagc tgagcaaccg ggagcgcgaa atagaccatc tgcaggcgca
       61 gctggataaa ctccgccgga tgaacttcgg cagtcgttcc gaaaaagtct cccgccgtat
      121 cgcacaaatg gaagccgatc tgaaccggct tcagaaagag agcgatacgc tgactggtag
      181 ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc cgcacccgta agccgttccc
      241 tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg gcgccgtgct gcccgaactg
      301 cggcggttca ctgagctatc tgggcgagga taccgccgaa cagctggagt tgatgcgtag
      361 cgccttccgg gttatccgga cggtacggga aaaacatgcc tgtactcagt gcgatgccat
      421 cgtgcaggca cctgcacctt cgcggcccat cgagcggggt atcgccggac cggggctgct
      481 ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg ctgtatcgcc agtcagaaat
      541 atacggccgg caaggtgtgg agctgaggcg ttcactgctg tcgggctggg tggatgcatg
      601 ctgccggctg ctgtctccgc tggaagaggc gcttcatggc tatgtcatga ctgacggcaa
      661 actccatgcc gatgataccc cggtccaggt actgctgccg ggtaataaga agacgaagac
      721 cgggcggttg tgggcgtatg ttcgtgatga ccgcaatgca gggtcagcgt tggcacctgc
      781 agtgtggttc gcttacagcc cggacagaaa aggcatccat ccgcagactc atcttgcctg
      841 cttcagcggt gtgctgcaag cggatgcgta cgccgggttc aacgagctgt atcgcaatgg
      901 tgggataacg gaagctgcct gctgggctca tgcccgccga aagatccacg atgtgcacgt
      961 ccgcatcccg tcag
//
LOCUS       DAZKFZ010000484          962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000484 DAZKFZ010000000
VERSION     DAZKFZ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.488"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>962
                     /locus_tag="ACHOQW_005115"
     CDS             <1..>962
                     /locus_tag="ACHOQW_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFP9575973.1"
                     /translation="YLSLSEIHRTLTNSTSFADISIPLLQTICPSITTIIKKTEYYGH
                     QLTNAMTVASVVKPYDFSNLGAINSIDKSVSDVPALHTIVEQAKYNLLSWNDFYNTHA
                     SIWDTIARQHKSTNIEFHPQSLLFDRDSKGKCLGLSLLYLDTGGYGGGYQKLRHNIDT
                     ASTLYQTKYNDNLKLSNRDDFFLRKTQRIITMSNELGNNRLKNAQLEVLELKDPILTE
                     GILYQRRISSLLITTEYHSLALQQISSFWRVTDPNFGHCDFHSLAQALTFIKNITSNR
                     NFSSLYGSGIVKIYFSESLNNWKYIKLPLVQTGSLLRDIYLTTP"
BASE COUNT          339 a          166 c          151 g          306 t
ORIGIN      
        1 tatttatcgc tgagtgaaat acatagaact ttaacaaata gtacttcatt tgcagatatt
       61 tctataccat tattgcagac catatgtccc agcataacaa caataataaa aaaaacagaa
      121 tattatgggc atcagttaac aaatgctatg actgttgctt cagtggttaa accatatgac
      181 tttagcaatc ttggtgccat aaatagtata gataaatccg tttctgatgt gcctgcattg
      241 catacaattg ttgaacaagc aaaatataat ttactttcat ggaatgattt ttataacact
      301 catgcgtcaa tatgggatac aatagcaaga cagcataaaa gtacaaatat cgaatttcac
      361 ccacaaagtc ttctttttga tagagattcg aaaggaaagt gccttggttt atctcttctg
      421 tatcttgata caggggggta cggaggcgga tatcaaaaac tgaggcacaa tattgacact
      481 gccagcacat tatatcaaac aaaatacaat gacaatctaa aattatctaa ccgtgatgat
      541 ttctttttaa gaaaaacaca gcgtatcatt acaatgtcca atgagttagg taataacagg
      601 ttaaaaaatg cacagttaga ggttctggaa ttgaaagacc ctatactaac tgaaggcata
      661 ttataccagc gtcgtatatc atctctttta ataacaacag aatatcattc acttgcatta
      721 caacaaatat cttctttttg gagagtaact gatcctaatt ttggtcattg tgactttcat
      781 tccttagcac aggctttaac gtttataaaa aatataacga gcaatcgtaa cttttcatct
      841 ttatatggaa gtggcattgt taaaatttac ttcagtgaat ctcttaataa ttggaagtat
      901 attaagctcc cccttgttca gactggttct ttgttaagag acatatattt aacaacccca
      961 ga
//
LOCUS       DAZKFZ010000485          960 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000485 DAZKFZ010000000
VERSION     DAZKFZ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.489"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..795
                     /gene="gadX"
                     /locus_tag="ACHOQW_005116"
     CDS             <1..795
                     /gene="gadX"
                     /locus_tag="ACHOQW_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP9575974.1"
                     /translation="AYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLV
                     SRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEML
                     RKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLM
                     SPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVF
                     RNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          279 a          181 c          213 g          287 t
ORIGIN      
        1 gcgtacgcaa gacataaata tattctcacc atggttaatg gtgaatatcg ctattttaat
       61 ggcggtgacc tggtttttgc ggatgcaagc caaattcgag tagataagtg tgttgaaaat
      121 tttgtattag tatcaaggga tacgctttca ttatttctcc cgatgctcaa ggaggaggca
      181 ttaaatcttc atgcacataa aaaagtttct tcattactcg ttcatcactg tagcagagat
      241 attcctgttt ttcaggaagt tgcgcaacta tcgcagaata agaatcttcg ctatgcagaa
      301 atgctacgta aaagagcatt aatctttgcg ttgttatctg tttttcttga ggatgatcac
      361 tttataccgc tgcttctgaa cgttttacaa ccgaacatgc gaacacgagt ttgtacggtt
      421 atcaataata atatcgctca tgagtggaca ctagcccgaa tcgccagcga gctgttgatg
      481 agtccaagtc tgttaaagaa aaaattacgc gaagaagaga catcatattc acagttgctt
      541 actgagtgta gaatgcaacg tgctttgcaa cttattgtta tacatggttt ttcaattaag
      601 cgagtcgcag tgtcctgtgg atatcacagc gtgtcgtatt tcatttacgt ctttcgaaat
      661 tattatggga tgacgcccac agagtatcag gagcgatcgg cgcagggatt gccgaaccgt
      721 gactcggcgg caagtattgt tgcgcaaggg aatttttacg gcactgactg ttctgcggaa
      781 ggaataagat tatagagttt tactcagaca taaaaaaaac ccggcatagg ggaccgggaa
      841 gaggatagtc tgccgtctcc agactaataa accgttataa cactccctgt tggcacggga
      901 aactttgtgc tctcagtaag ttaaatataa cttttactgg aaataagatc agccattttt
//
LOCUS       DAZKFZ010000486          957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000486 DAZKFZ010000000
VERSION     DAZKFZ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.490"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>957
                     /locus_tag="ACHOQW_005117"
     CDS             <1..>957
                     /locus_tag="ACHOQW_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9575975.1"
                     /translation="DALAQKIREGWQPYGGPFSSYTDDGAALIQAIVAEGDVSTPVVV
                     KPTGGEGAVISATSDPGYYFVVVLAGQSNGMSYGEGLPLPETYDRPDPRIKQLARRST
                     VTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALK
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG"
BASE COUNT          228 a          220 c          300 g          209 t
ORIGIN      
        1 gatgcacttg cgcaaaaaat tcgtgaagga tggcaaccat atggtgggcc gttttcttcg
       61 tatacggatg atggcgcagc acttattcag gcgattgtcg cagaaggtga tgtgagcaca
      121 cctgttgtgg tgaagccgac aggtggagaa ggtgcagtaa tcagcgccac cagcgacccc
      181 gggtattact ttgttgtggt tctggcaggg cagtcaaacg gcatgtcgta tggtgaaggt
      241 cttccgctgc cggagacata tgaccgtccg gacccgcgca ttaagcagct ggcgcgtcgc
      301 agtacggtga caccgggcgg tgtcgcctgt aaatataacg acatcattcc ggcggaccat
      361 tgtctgcatg atgtgcagga catgagccgc cttaaccatc cgaaagcgga cctgtcaaag
      421 gggcagtacg gaaccgtggg gcaggggctg catatcgcca aaaaattgct gccgtttata
      481 ccggcgaatg cgggcattct gctggttccg tgctgtcgtg gtggttcagc gttcaccacc
      541 ggagctgatg gcacatacag tgacgcgagt ggtgcctcgg agaattcaac ccgctggggt
      601 gtggacaagc cgctgtataa ggaccttatc ggtcgaacaa aagcagcact gaagaagaat
      661 ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg
      721 ccggcaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg
      781 gatatggcag gtcagtgcgt cggtggctct gctggcggtg ttccctggat atgtggagat
      841 acgacgtatt tctggaagca gaagaacgaa tccacgtacc agacggtgta cggcagctac
      901 aaaaacaaaa cggaaaagaa tatccatttc gtaccgttca tgaccgatga gaacggg
//
LOCUS       DAZKFZ010000487          955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000487 DAZKFZ010000000
VERSION     DAZKFZ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.491"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>955
                     /locus_tag="ACHOQW_005118"
     CDS             <1..>955
                     /locus_tag="ACHOQW_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9575976.1"
                     /translation="GRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQ
                     NSEQPEPVKVTADEVKKIMQAANISQPDANQLLAASRGEFVAGISDPNDPKWVKGIET
                     RDSVNQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNC
                     PDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGET
                     ADHSIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFS
                     KTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDAL"
BASE COUNT          321 a          227 c          241 g          166 t
ORIGIN      
        1 acggtcgcgc aaacaaactt tctggtctgg ataaggcgct ggctgccggg gctactgaaa
       61 tcacaaaaga agaatatttt gcccgaaaaa atggcacata cacaggctta ccgcaaaatg
      121 caaataccgc acaaaattct gaacaaccag aaccggtaaa agttaccgct gacgaagtaa
      181 agaaaattat gcaggcagcc aatatcagcc agcctgacgc caatcagttg ctcgccgcat
      241 cacgtggtga atttgttgca gggattagcg acccgaatga tccgaaatgg gtgaagggga
      301 ttgaaacccg cgattctgtg aaccagaacc agcaagaaac ggaacagaac gaccagaaag
      361 cggaacaaaa cagcccaaat acgcaacaaa acgagccaga aacgaaacaa cctgagccag
      421 tagcgcaaca ggaaccggaa aaagtctgca ccgcctgcgg tcagaccggc ggcggcaact
      481 gccctgattg tggcgcggtg atgggcgacg caacatacca ggaaacattc gatgaagagt
      541 atcaggttga agttcaggaa gatgatccgg aggaaatgga aggcgctgaa catccacaca
      601 aggagaacac tgacggcaat cagcatcacg atagcgataa tgaaactggc gagacggcag
      661 atcactcaat taaggtgaac ggtcatcaag aaatcacatc caccagcagg acgtgtgacc
      721 atctaatgat cgaccttgaa accatgggaa aaaatcctga tgccccgatc atctcaatag
      781 gtgcaatatt tttcgatccg caaaccggag atatgggacc ggaatttagt aagactatcg
      841 atctggaaac tgctggcggg gtcattgatc gggacaccat taaatggtgg cttaagcaat
      901 cacgcgaagc gcaatctgcc attatgaccg atgaaatccc gttagatgat gcact
//
LOCUS       DAZKFZ010000488          954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000488 DAZKFZ010000000
VERSION     DAZKFZ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.492"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..139)
                     /locus_tag="ACHOQW_005119"
     CDS             complement(<1..139)
                     /locus_tag="ACHOQW_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9575977.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NN"
     gene            complement(485..664)
                     /locus_tag="ACHOQW_005120"
     CDS             complement(485..664)
                     /locus_tag="ACHOQW_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFP9575978.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            complement(731..>954)
                     /locus_tag="ACHOQW_005121"
     CDS             complement(731..>954)
                     /locus_tag="ACHOQW_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575979.1"
                     /translation="MNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPS
                     PIIPLNWSKASMTLMLKEMSNLINDEGIK"
BASE COUNT          271 a          194 c          148 g          341 t
ORIGIN      
        1 ggttattacc aatcgtaata acaagggtta catccttgtt ttcatttccc cgtaagaacc
       61 acatttcagg gtccagtagg gttaacttct gtccttgaag caaaacattc attggaagta
      121 acagcccttc ttcaatcatg tttcatctcc ttattccata ttctcattct ccataaactt
      181 gctataaaaa catgattaaa aagtgatgat tactatttat aaaataatga ttttgttatg
      241 agcgaatagc tatcagccac ttacactagt acgtcatcct gtccatatac agatttgtaa
      301 ctgttgatat gttttactta aacacacctc gtattactta taacacttgt ttggtaacta
      361 ttttcaatac aaatcatatt tgttagttct tcattaacac actgcgagtg ttttgttact
      421 ccccgcatat attttattct cttgttcgat tctatttttc ttaatcgatc atataatttt
      481 cttgttagtc ggatttacga atcatgagac ttataatgga aggaatgaga ctaagcggcc
      541 aggttaaacc tgtaattatt gcagcaaaca accgatatgt tactttttct tccgagaaga
      601 taaatattaa agtcgatgtc acaaccagac ctatcaggta agctaacagt aatttattga
      661 tcacttttac ctcccctttc gatttctctt tgagcggtcc ttttaatcca ggaccgcctc
      721 attatgaaaa ttatttaatt ccctcatcat tgataaggtt ggacatctct ttcaacatga
      781 gagtcattga agctttcgac caatttagcg gaatgatagg gcttggcgct tcatgcaaag
      841 ttcccgactt gtggatatag ttatagcttt cgggaagtgc catttctggt acgctcctgc
      901 catttgcacc aatcatattc tcgcctgtta tttgggccaa tgatctattc ataa
//
LOCUS       DAZKFZ010000489          954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000489 DAZKFZ010000000
VERSION     DAZKFZ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.493"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(344..>954)
                     /gene="map"
                     /locus_tag="ACHOQW_005122"
     CDS             complement(344..>954)
                     /gene="map"
                     /locus_tag="ACHOQW_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFP9575980.1"
                     /translation="FSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQS
                     NFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVIS
                     KETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFNT
                     QTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          296 a          199 c          162 g          297 t
ORIGIN      
        1 gtagtcgata aacggctgaa catgacatcg accaaataaa tcacaatctg atttttgatg
       61 caagaaaata atgtgctaaa aacacacatc gggtaacaaa ataaaaataa ttatattata
      121 taaaagtaaa caccacgtga aatcttaata aaacatttca taaataaaat tgcagcaaac
      181 attcatttat attaaaaaac acagccaaag aaaaccaatt aagcaaacta taaatcttaa
      241 ttaataataa agtttattaa gtttctttat cgcgtttttt cttacatcaa caaaggccat
      301 gctatacacc aggaagctta atttatacct gacaaggtta tccctacaat cgggtatcct
      361 gtacatgctc taaaatcgca ttctgagtaa tcgcttttat ctcactgact ttgaagccgc
      421 taaagttgcg ttcaaatatt tcttgtgaga cttgttctat catcttcgtc tgggtgttaa
      481 aacgtgttat tggatccact cttttgccgc actgctcatc aactttctga ttaaagtatt
      541 tattggactg caagaagtga ccaattgatg attgtgcccc gttcatatca agaggttgct
      601 gtaacgcatt ttcaacagct ctaaaaatgt gacctttagt ctccttacta atgaccgaat
      661 tattactcct gcaatagtca tccagagttc tatttactgt tctggatata taagtcgtct
      721 gctcctgctg gaaccattgt tgggtatcgc cattacgagt ttgtttggct acagcctgca
      781 ggagttggtt agtcagtttg ccatgattaa tcacaaagtt cgactgcacg ggcatgaacc
      841 tcatacccgt ggctcccgta cccgcttgca ttgcagctct ggtaaccgca ggtttaagcg
      901 tctgtgcagt cgcctgaact aacgatctgc ctgccattgt cattggacta aaca
//
LOCUS       DAZKFZ010000490          948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000490 DAZKFZ010000000
VERSION     DAZKFZ010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.494"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..175)
                     /locus_tag="ACHOQW_005123"
     CDS             complement(<1..175)
                     /locus_tag="ACHOQW_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9575981.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSE"
     gene            complement(187..>948)
                     /locus_tag="ACHOQW_005124"
     CDS             complement(187..>948)
                     /locus_tag="ACHOQW_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9575982.1"
                     /translation="LISAEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLHGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDIAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          250 a          223 c          170 g          305 t
ORIGIN      
        1 cctctgaatc accatgttgt gatttatact tcagtttcat caacttttct tcacttaaat
       61 cataggtcat tgctgtagac cccatcatac tttcgttttg ttttattttc acatcagcac
      121 catgcaattg acaaataaaa gagaaataaa aaaatagtaa caagcatgct ttcatacatc
      181 tacaacctat cttatatcac tgggtttttc tggcgtacct tgcatattag aaatggggtg
      241 cccagatggc ccatatgtta tccaattacc aaggtctttt gttgttatag ccattagatc
      301 cttatttgaa ttaaggttcg ttggtgctgt tagcactgta tccattttta acttaccatt
      361 agttcctcgc ccccaccacg tccatagcgt gtcaggtcct attacatttg tatgtaactc
      421 tttagctaat tggctagcaa atgaattttg tcctttacct gtattacaag aaaataacca
      481 aactgtcatc ccatctttat aattcccatc aaatttaatt agatatgcta tatctttagc
      541 tgtcatgata ctgcgcgttg cggattcaat agatgtgggg gtaccatgcc ccccgattgt
      601 gaaaacgcct gggatattaa tctcatcagc tacagaatgg ataagatcac tttcggggta
      661 taaatttata tcaaccaaac cttgcggatc tacatttatg accggattca acggatactg
      721 ataaaaattc catcccccct tcagtccaat cggatcctga gtgatatatc gcccgtgcag
      781 cgggtcataa tagcggtggc ggttgtaata caggccggac tcctcatcat actgctgccc
      841 cggcaggcgg ataagctgct gcagctgatg cgggttctct tcattcagca ggttgcccca
      901 ttcatcgtat tctgcgcacc attctgttgc cccttccgca ctgataag
//
LOCUS       DAZKFZ010000491          948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000491 DAZKFZ010000000
VERSION     DAZKFZ010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.495"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>948
                     /locus_tag="ACHOQW_005125"
     CDS             <1..>948
                     /locus_tag="ACHOQW_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9575983.1"
                     /translation="DGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPE
                     KEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFM
                     LRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRI
                     AAPAAPSQIELTPGYFQITAVPKLAVYDPTVQFEFWFSEAKIADTSQVETSARYLGTG
                     SQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHL
                     AQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTLE"
BASE COUNT          218 a          241 c          310 g          179 t
ORIGIN      
        1 gatggtgtgg agacatacgg tgtatgggga ctctccctgc cgtcactgcg tcgtcgcctg
       61 ttccgctgtg tctccatccg ggaaaacacg gacggcacct ttgccatcac ggcggtgcag
      121 cacgtaccgg aaaaagaagc catcgtggat aacggggcca gctttgagcc gcagtcaggc
      181 accctgaaca gcgttattcc accggcagtg cagcacctga cggtggaggt gagcgcggct
      241 gacggtcagt atctggcaca ggcgaaatgg gacacgccga aggtggtgaa gggcgtgagc
      301 tttatgcttc gcctgaccgt ggccgcggat gacggcagtg agcggctggt cagcacggcc
      361 cggacgacgg aaaccacata ccgcttcacg caactggcgc cggggaacta caggctgaca
      421 gtccgggcgg taaatgcgtg ggggcagcag ggcgatccgg cgtcggtatc gttccggatt
      481 gccgcaccgg cagcgccgtc acagattgag ctgacaccgg gctattttca gataacggcg
      541 gtcccgaaac tggctgtata tgacccgacg gtacagtttg aattctggtt ttcggaggca
      601 aaaatcgcag acacatctca ggtggaaacc tctgcccgtt atctggggac cggcagtcag
      661 tggagtgtat ccggcccgca cattaagccc gggaaggatt tctggtttta cgtgcgcagc
      721 gtcaacctgg tggggaaatc tgcgtttgtg gaagtcagcg ggcagcccag caatgatggt
      781 gaagggtatc tggaattttt ccgggaaaaa ataggaaaac tgcatctggc tcaggggttg
      841 tgggaactga tagataacag ccagcttgcg gatgagatgg cggagatgaa gaccagcatc
      901 acggaaaccc gcaatgaaat cacacagacg gtcagtaaaa cgctggag
//
LOCUS       DAZKFZ010000492          945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000492 DAZKFZ010000000
VERSION     DAZKFZ010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.496"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(24..473)
                     /gene="sctT"
                     /locus_tag="ACHOQW_005126"
                     /pseudo
     CDS             complement(24..473)
                     /gene="sctT"
                     /locus_tag="ACHOQW_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(475..735)
                     /locus_tag="ACHOQW_005127"
     CDS             complement(475..735)
                     /locus_tag="ACHOQW_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9575984.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(746..>945)
                     /locus_tag="ACHOQW_005128"
     CDS             complement(746..>945)
                     /locus_tag="ACHOQW_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9575985.1"
                     /translation="GFYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAM
                     DGWTMLSKGLILQYFDLSINP"
BASE COUNT          334 a          206 c          158 g          247 t
ORIGIN      
        1 ggccataaat tataactttg ctaaagagtt tccagaataa agttcaatcc accgttagta
       61 agataaaccg ctgcagagaa aaggttaaat aatcttgcta attccgaggt atcgacacct
      121 gtagccggat ccaaagtact acttaacgtt gcaccgcgtt ggttatcgat aaagctacct
      181 atagcaagga aaatccaaaa tggagatgct aataagcaag caatgaacag ccctatgata
      241 atttctcttg cagctaacaa agcgatatcg atttcattta aggtcgataa ttcatagtgg
      301 taatgctgaa ctaatgcaca tgataccact attattattg gatgacggac tactgttggt
      361 atattcccac tggcgaaaaa tggtaacaaa tagaaagttg gtgccaatcg gcaaaaaccg
      421 agcgctgtag cagccagtag actatgcaac tgatagagta tcacctcccc catgctatcc
      481 tctcgcaaat gctaaattca gcatttcaat accaaaagaa tataattttt caccatacca
      541 tccgctcatt aaaaaaaagc aaatacttac gcacaataat ttcacgccaa atggcaacgt
      601 ctgttcttgc aactgcgtta ccgtttgaaa aagccctacc aataaaccaa caaatgtcgc
      661 tactgcaata ggtcctgcag acataactaa aatcagatac agcgccctat tccctgcaaa
      721 aacaatgtca tccatatttt tttcctcagg gattaatgga taggtcaaaa tattgtaata
      781 tgagtccttt tgataacatt gtccaaccgt ccatcgcgac aaacagaatt aattttatcg
      841 gtgtagatat ggttaccgga ctcatcatca tcatacctaa cgttaacaga acgctggata
      901 taaccagatc cactacaaca aagggtaaat agatataaaa accta
//
LOCUS       DAZKFZ010000493          944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000493 DAZKFZ010000000
VERSION     DAZKFZ010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.497"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(112..801)
                     /locus_tag="ACHOQW_005129"
     CDS             complement(112..801)
                     /locus_tag="ACHOQW_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9575986.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(816..938)
                     /locus_tag="ACHOQW_005130"
     CDS             complement(816..938)
                     /locus_tag="ACHOQW_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9575987.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          252 a          236 c          187 g          269 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaccc actcggcagc
       61 gggtttatac attttttaca acataccaaa tttgcatgaa gtatatggct tttaatccag
      121 ttttgcaata ttttgctgta aaaatgctgc cttttgtttt gaacgtgttc tcgtcacaag
      181 caataaagca tcactatcaa gctgtagaaa aatgtgcttc attgcaaccc agcgttcagt
      241 aaatgtctcg gaccagtttt ttgttgtcac tcccaccaat gatgccagtg tctggtattc
      301 ataggcctca cgccctgcaa gttcgctctt cacatcctgt gcagccagcc agattaacgt
      361 cttcaggcga tccagtgtct tacctgcaat ttttctgtta cttaacaaat ctttaaactc
      421 gctccatgcc cattgcgtta tggtgacctg atgcccccat cgaacgcttt cgctgtaaca
      481 ccaaagcaac catgctttct gatgttcatc gagagacaaa accgcgcggc gccatgaaga
      541 ggttgagaat tcaaccgggc tgaccaaagc aatggatgaa ccttttgcgt acgactgctt
      601 accggaagtc ggcgtattat ccagcgtaat catcttgcca gttaccacat ccagaatgcg
      661 cggcttcttt cgtttgtatg taccagtatc aaattgtgca tgctcctgcc aggcttcgag
      721 ctggcctttc gttgctccgt tcaagtcagc agtagctgcc ataagttgct cacgaacata
      781 ctgtaaatat tgggtattca tgcagtaaat cctttctata ttttggcata attcttcaac
      841 attcggtaat cgttcaaaac cgaatcgggg aaacgacata agcacaggag cccccagcga
      901 cagcgaagga gttctgatat ataagactca gacatcattc attc
//
LOCUS       DAZKFZ010000494          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000494 DAZKFZ010000000
VERSION     DAZKFZ010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.498"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..930
                     /locus_tag="ACHOQW_005131"
     CDS             <1..930
                     /locus_tag="ACHOQW_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9575988.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDIL
                     FTGGSGPDNKILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRP
                     SRTAEEEVQAQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWP
                     GKHADEIKNVKASLKNTLIDVKKRCMPTSGIPR"
BASE COUNT          285 a          175 c          282 g          201 t
ORIGIN      
        1 aggctggagc gggaactgcg gcagggcagc gtgagtgaag aaagccagca gtggcttgcg
       61 cagtgcgggc tgacggcgga acagatggcc gcgcagctgg aggcggaata tatcccggag
      121 cggaaacttc atctttacca ctgcgaccac cggggactgc cgcaggcgct catcagcccg
      181 gaaggggaaa cggcgtggca gggggagtat gacgagtggg gaaacctgct gggcgaaacc
      241 agcgcgcagc acctgcaaca gccgtaccgt ctgccgggac agcagtatga tgaggagtcg
      301 gggctgtatt acaaccgtca ccggtactat gacccgctac aggggaggta tatcacccag
      361 gatccaatag atataaaagg aggatggaat ttatattctt atgcgcttaa tccggtaagt
      421 tggattgacc cattaggatt gacgcagtgc gattccgaag ggtgtaataa tgatatatta
      481 tttacaggag gaagtggtcc cgataataaa atacttaatg aattaggtcc gagagatggc
      541 attgacggtt tgggttcaca aaacatgaaa atgtatagtg ggctattagg gggcgatatt
      601 ttaaaaccgg ggattctggg cggtttgacg cttggtagtg tgacacaacg accatcgcga
      661 acggctgagg aggaggtgca agctcaaata gaatatttag catataagaa gcgttgtgag
      721 caaaaacctg atagtaattt aagtcgttgt gctgccgcga tctttcaaaa gagcagaaaa
      781 gaagattgtt taagaatgag acaagaatgg gatgataaat ggtggcctgg caaacatgcg
      841 gatgaaataa aaaatgtgaa agcatcgttg aaaaacacac ttattgatgt taaaaaaaga
      901 tgcatgccaa ctagtggcat tccgagatag gtatccttaa aat
//
LOCUS       DAZKFZ010000495          938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000495 DAZKFZ010000000
VERSION     DAZKFZ010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.499"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..245
                     /locus_tag="ACHOQW_005132"
     CDS             <1..245
                     /locus_tag="ACHOQW_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575989.1"
                     /translation="SAVFASRHGRRSHAFKSDWFRHAPCTEEQAEWLIQNYRRRGYEF
                     RKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            247..>938
                     /locus_tag="ACHOQW_005133"
     CDS             247..>938
                     /locus_tag="ACHOQW_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9575990.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT          203 a          266 c          261 g          208 t
ORIGIN      
        1 attcagccgt ctttgccagc aggcacgggc ggcgttctca tgcattcaaa tctgactggt
       61 tccggcacgc cccatgcact gaagaacagg ccgaatggct gattcagaac taccgcagac
      121 gtgggtatga gtttaggaaa gccctcagcc tcgattatcg tcactggata atctacgtca
      181 ggctgccgta ctccgagcgc ccaccgcgtc cgtcccgcac attccagcaa cgcatctgga
      241 ggtaacgtgc gggtattact tcgacctgtt ccggtaccgg aacttggggt ggtggtcctt
      301 aaaccaggcc gtgaatccat gcaggtattc cataaccctc gagtgcttgt ggagccggag
      361 ccgaaaagca tgcgcggtct gccgtccggc atcgtccctg ccgttcgcca gccgctggcg
      421 gaggataaat cattactgcc gtttttcagc aatgagcgtg tgattcgtgc tgttggtggc
      481 gctggtgcac tgtctgactg gctgttgcgt catgtcaaat cctgccagtg gcctcatggt
      541 gactatcatc acagtgaaac cgttattcac cgttatggta ccggcgcaat ggtgttgtgc
      601 tggcactgcg acaaccagct gcgtgaccag acatccgaat cactcgagca acttgctcat
      661 caaaacctgt cagcatggat gattgacgtc atcggtcacg caataagcgg tacgcaggag
      721 cgtgaattat ctctggctga attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg
      781 ctaccggaag cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat ccgctcaatg
      841 taccgtgaaa gcgacatcgt accgggagag cagaccgcca ccatcatact gaagcagcgc
      901 acaaaaaatc ttgcgccgct gcctcacgcc caccagca
//
LOCUS       DAZKFZ010000496          919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000496 DAZKFZ010000000
VERSION     DAZKFZ010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.500"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..912
                     /locus_tag="ACHOQW_005134"
     CDS             <1..912
                     /locus_tag="ACHOQW_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFP9575991.1"
                     /translation="FLQIFPLHDEIGFNFKDLPGPLKNALSNSNISTTAVSTIASTGT
                     SATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANY
                     TEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANALGALG
                     GFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPGLKPDTILWWAGATLGAADLNKA
                     EFEVARFTDYPRIWWHAREGAIFPNKADIEHATGADIRAMEEGIPVGQRHPNPEDVVI
                     DIESNGLPHHNPSNHVDIFDIIQETRV"
BASE COUNT          281 a          190 c          209 g          239 t
ORIGIN      
        1 tttttacaga tattccctct gcatgatgaa ataggtttta attttaaaga ccttcctggc
       61 ccgttaaaaa atgcattaag caacagtaat atatcaacca ctgcagtgtc gactattgca
      121 tcgactggaa catcagccac tacttcgacg gtaaccaccg agccaaaaga cccaatacca
      181 tggtttggat taacagctca agtggttcgt aatcatggtg tagaacttcc tatagtcaaa
      241 actgaaaatg gatggaagct tgttggagaa acaccactta ctcctgatgg gccgaaagca
      301 aattacacgg aggagtgggt tatcagaccg ggagaagcag attttaaata tggtgcatct
      361 ccattacagg caactctagg gctggagttt ggcgcacatt tcaagtggga tttagataac
      421 cctaatacta aatatgccgt tcttaccaat gctgccgcaa atgcgcttgg tgctttaggg
      481 ggatttgcag tatccagatt tgctagtaca gatccaatgt taagtcctca tatcggtgca
      541 atggttgggc aagcagcagg gcatgccata cagtataata cccctggatt aaagccagac
      601 actattttat ggtgggctgg tgcgacactg ggggctgccg atttaaacaa ggccgagttt
      661 gaagtagcta gattcactga ctatcctcgt atatggtggc acgcaagaga aggagctatt
      721 ttccccaata aagcagatat tgaacatgcc acaggtgctg atatacgcgc aatggaagaa
      781 ggtatccctg ttggacagcg gcatccaaat ccagaggatg tggtaatcga tatcgaaagc
      841 aatggcttac cacatcataa tccatcaaat catgttgata tctttgatat aatccaagaa
      901 acaagagtct aagctttaa
//
LOCUS       DAZKFZ010000497          918 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000497 DAZKFZ010000000
VERSION     DAZKFZ010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.501"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..671
                     /locus_tag="ACHOQW_005135"
     CDS             <1..671
                     /locus_tag="ACHOQW_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9575992.1"
                     /translation="FLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAV
                     MRIMASYAFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVY
                     PSYWEEWKKVREVVKVACPECGGKGEVSTACKDFRGRGVAIHREESVKRGMPVIRDCQ
                     RCGGRGYERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLK
                     KVTR"
BASE COUNT          252 a          167 c          261 g          238 t
ORIGIN      
        1 cgttccttgg gaaaatgggc attagtatga atgacagaga gcgggcaaca gaattgctga
       61 cagaatatgc actcagtcgg tgtgatcgcg tggcggcgtt aagaaaactc ccggcagaaa
      121 taaaaccggc agtgatgcgt attatggctt cgtatgcgtt tgaagattat gcccgtagcg
      181 cggcgagcaa aaaacagtgc ccctgttgtc acggaaaaaa atttattgaa agcgaggttt
      241 ttacaaacaa gatccagtat ccggatggta agccgccagt gtgggcaaag tgcacaaaag
      301 gcgtgtatcc gtcttactgg gaggaatgga aaaaagtcag ggaggtggta aaagttgcct
      361 gtccggagtg tggagggaag ggggaggttt ccaccgcctg taaagatttt cgtgggcgcg
      421 gtgttgccat tcatcgtgaa gagtcggtaa aacgtggtat gcctgttatc agagactgcc
      481 agcgttgtgg tggtcgtggc tatgaaagat taccttcaac ggaggcattt aatgccatat
      541 gtaatgtaac cgatgccata tctcttgata catggaaaaa aacagttaaa cgtttttacg
      601 atacgctggt ggtgcagttt gatattgaag aagcatgggc agaacaacaa ctgaaaaagg
      661 tgaccagata gctttgttga tttttcccga atctgtggta aatttgccct aacgatgggc
      721 gttttatgcc tgacgttaga agatttttta cacccgtcgc caggcgggtt tttttatgac
      781 tgaaatcacg ccagtacagt aaacgcgctg gtggttgtga ataccggtct ttctgcttgc
      841 tggctttttc gacaagagtt attggtgtgt cacgttaacc ggaaaaagga aagtttgaga
      901 aacgcgatct ggcacagg
//
LOCUS       DAZKFZ010000498          917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000498 DAZKFZ010000000
VERSION     DAZKFZ010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.502"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            45..845
                     /locus_tag="ACHOQW_005136"
     CDS             45..845
                     /locus_tag="ACHOQW_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9575993.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          293 a          177 c          208 g          239 t
ORIGIN      
        1 gctaaatatt ctaatcatta tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc
       61 tttgttgcat atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg
      121 atgaaaagaa gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata
      241 ttcaagcagc gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg
      301 ttcaatccgg caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata
      361 ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca
      421 aagataaaaa tgagaacgag gatgttgccc gtgctgagaa tatgaactgg atgcaggcac
      481 tgcgttttgt ggctgcaaaa ggacatcaga aagcgattat tgtttaccag gatatgttgc
      541 agacaggaaa atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa
      601 aactcactga cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa
      661 caggtgagtg ggctacctgg tcgccggtga attatgaatc gagagtaatt tttccgccgg
      721 ctggaataac aaaaacgagc aataaagaac tatcaaatga ccatgttacg gaagcgcaac
      781 tgtttaattt gaaacaaaaa acatatgtat cgatggtgaa agacttggtg agtcgattcc
      841 agtaatcgtt attgttatgc tgccgcctgt agtttagggt ggcagcaaac aggagttgag
      901 tatttctata atgtaaa
//
LOCUS       DAZKFZ010000499          915 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000499 DAZKFZ010000000
VERSION     DAZKFZ010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.503"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            42..575
                     /locus_tag="ACHOQW_005137"
     CDS             42..575
                     /locus_tag="ACHOQW_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9575994.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            625..>915
                     /locus_tag="ACHOQW_005138"
     CDS             625..>915
                     /locus_tag="ACHOQW_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9575995.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLP"
BASE COUNT          297 a          177 c          182 g          259 t
ORIGIN      
        1 aaaacgagtg accttccata ccagttaatt aaggatgatt tatgaaaaag tatattattc
       61 ccttcgttgc gacaacgttg atgtttagcg caacaacctt cgccaaggat gtcgatcaag
      121 gaacactaac cattagagga gaagttgttg atacaacgtg ttactttgtt aatggtgatt
      181 cgacaacaga tatcgttgtc gaagatgttg taaccagtgc aatgaatcaa cttatcaata
      241 atgaaatcta tactttaaaa accggttcaa cctctcatga cttagaaatt cagtgtgacg
      301 gaaatacagc cccaagtatc gaagtactag catcagagtt tgatgctaat aactttacgc
      361 tgaataaagg aactgctgag aacattggct ttgccatttt cctcgatgat gggactttga
      421 ccagaatgag accagggtta aaaacaccgc tgaaagataa tggctctggt caatattttc
      481 tcaatttaaa tgcacaatat gcccgcacaa gtggcaatcc tgttacaaaa ggctccgtag
      541 agtcaaccgt aacgattaaa atcagtgcag attaacgttt tttgccggta gctctgctac
      601 cggtattatt tacggagtga ttacgtgaga ataataacag cattactgat gtcatttttt
      661 attttacctg ttcatgccgg tgtggtcatc tatggtacgc gcattattta tccggaaaaa
      721 aacagagaag ttctcgtaca gttaatgaat caaagtaaaa atgcctcttt gattcaggca
      781 tggatcgatg atgggaacac cactatcgcg ccagaaaaaa ttcaggtcac atttattctc
      841 acaccgccag taagtcgtgt agcaggtggt tctggccaac aacttaaaat aagaaaaatg
      901 ccaaacaatt tgcct
//
LOCUS       DAZKFZ010000500          901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000500 DAZKFZ010000000
VERSION     DAZKFZ010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.504"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..843
                     /locus_tag="ACHOQW_005139"
     CDS             <1..843
                     /locus_tag="ACHOQW_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9575996.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
BASE COUNT          208 a          223 c          261 g          209 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc
      481 cctgtgacgc ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac
      541 ggttctttat gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg
      601 aaaaggctga aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg
      661 gcacataccg acgcctggcg tcagcagggc tatgaagaca cgatgctggc gatggagcat
      721 atccggaaac cgctggcagc acgtcaggca ctgacccggt cagaggcggt attgcagaaa
      781 agcctggata taacggaaga ggcagatttg gcactgagaa acgcgatggc ccggattaaa
      841 taaagacgcc ccaaagaaac gccaccgcct gtggtggtgt ttttcctgtc tggtgttatt
      901 g
//
LOCUS       DAZKFZ010000501          899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000501 DAZKFZ010000000
VERSION     DAZKFZ010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.505"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..361
                     /gene="ler"
                     /locus_tag="ACHOQW_005140"
     CDS             <1..361
                     /gene="ler"
                     /locus_tag="ACHOQW_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFP9575997.1"
                     /translation="NNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQKQITY
                     YNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNGMKKE
                     DFLVKDTEEEIIPLKNI"
     gene            376..594
                     /gene="escE"
                     /locus_tag="ACHOQW_005141"
     CDS             376..594
                     /gene="escE"
                     /locus_tag="ACHOQW_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFP9575998.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            607..>899
                     /gene="cesAB"
                     /locus_tag="ACHOQW_005142"
     CDS             607..>899
                     /gene="cesAB"
                     /locus_tag="ACHOQW_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFP9575999.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETLSPARSKSQSTMNQRN"
BASE COUNT          381 a          134 c          190 g          194 t
ORIGIN      
        1 aaataattca catacaacaa gtccatacat tcagcttata gagcaaattg cagttctaca
       61 gcaggaagca aagcgactgc gagagcagga agttcaaagt gtaattgagt cgattcagaa
      121 gcagattact tattacaata taaccttaca agagctggga tatactaatg tgcctgatga
      181 tggactcgct cgccggaact catcgaaagg tgtttactac cgcaatgaag aagggcagac
      241 ctggtcgggc gtaggccgac agccacgctg gcttaaagaa gcactgttga atggaatgaa
      301 gaaagaagat tttcttgtga aggacactga agaagaaata ataccgctga aaaatattta
      361 acatgaaata attaaatgat aacgataact gagctggaag atgaaataat aaaaaataaa
      421 gaagccgcaa atgtttttat tgaaaaaata aacgacaaaa agaacgaaat ccatgaaaaa
      481 atgaaacacc ctttggataa agttacctac aatgaagcaa aggaactgct cattgcgtgt
      541 gatgcggcaa ttaggactat agagataatg cgaatcagga ttaataataa atagaggatg
      601 agtattgtga gccaaacaag aaataaagaa ctattagata aaaaaataag atcagaaatt
      661 gaggcgataa aaaaaataat cgccgaattt gatgtcgtaa aagaaagcgt taatgagtta
      721 agtgaaaaag caaaaacaga tcctcaggcg gcagaaaaat taaacaaatt aattgagggg
      781 tacacatatg gagaagagcg aaaattatat gattctgccc taagtaagat tgaaaaacta
      841 atagagacac tgagtccagc aagatctaaa agccaatcaa caatgaatca acggaatag
//
LOCUS       DAZKFZ010000502          894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000502 DAZKFZ010000000
VERSION     DAZKFZ010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.506"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..465
                     /locus_tag="ACHOQW_005143"
     CDS             <1..465
                     /locus_tag="ACHOQW_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFP9576000.1"
                     /translation="KHSNNKDREKKYRDGDKINGPQAHSLDITNERRFADNRTMFTQH
                     IEKQRNVNTLNQNDINNSANNANVRENELTYQFQRWGQNHTVRILESSEGIRLKPSDT
                     LVSDRLHEAQHNDVTAQRWVLTEQDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            425..>894
                     /locus_tag="ACHOQW_005144"
     CDS             425..>894
                     /locus_tag="ACHOQW_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFP9576001.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPT"
BASE COUNT          321 a          159 c          199 g          215 t
ORIGIN      
        1 aaacacagta ataataaaga tcgtgaaaaa aaataccgcg atggagacaa aataaatgga
       61 ccgcaggccc attcattaga cattactaac gaaagaaggt ttgctgataa cagaaccatg
      121 tttactcaac atatcgaaaa gcaaaggaat gttaatacgc tcaatcagaa tgatataaat
      181 aattctgcta ataatgccaa tgtgcgcgaa aatgaattga catatcagtt tcagcgttgg
      241 ggacaaaatc atacggtccg gattctggaa agtagtgaag ggatacgact aaaaccatca
      301 gacacacttg tctccgacag actccatgaa gcccaacaca atgatgttac agcacaacgt
      361 tgggtattga ctgagcaaga tgaacgacaa ggacaacgac atcagcctca tgaggagcaa
      421 gaaaatgaag gcaaattcga aaacgatcag aaggatgaaa gttaatgttc ggtctgagga
      481 aagtaaatcg aaacactcta cgtttgaaac cacttttcaa aattggaaag aaaatggcga
      541 agatgttgca ttattaatgc ccgaatttag tgctaaatgg ctaccgatag cagaagagag
      601 tggcagctgg tctggatggg tgttgcttcg agaaatattt cccttaattt ctgccgagct
      661 cgctgggatg gctttaatgc cagaaacgga aaggttaata ggggaatggc tcagtttatc
      721 cagttccccg ttaaatctaa agtatcctga actaaaatat aatcgtttgt gcgtaggtaa
      781 agtatttgat ggagtattga gtccagctca accactgata agaatatgga caggggaatt
      841 gaatctttgg ttagataaag tcacagtctg ccaatacgaa aacgctccaa cttt
//
LOCUS       DAZKFZ010000503          889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000503 DAZKFZ010000000
VERSION     DAZKFZ010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.507"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..447
                     /gene="yhaJ"
                     /locus_tag="ACHOQW_005145"
     CDS             <1..447
                     /gene="yhaJ"
                     /locus_tag="ACHOQW_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP9576002.1"
                     /translation="APDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTR
                     VKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKD
                     IAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK"
     gene            complement(498..854)
                     /locus_tag="ACHOQW_005146"
     CDS             complement(498..854)
                     /locus_tag="ACHOQW_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9576003.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT          270 a          195 c          229 g          195 t
ORIGIN      
        1 gcgccggata tgcattttcg ttcctcgtcg gagatcaact cgcgcaagct ctatacgtta
       61 atgaacgtct acgttgccgc gcctgatcac ccgattcatc aggagccgga accgttatct
      121 gaagtgacgc gtgtgaaata tcgtggaatt gcggtggcgg ataccgctcg tgagcgcccg
      181 gtgttgactg tacagctgct ggacaaacag ccgcgcttaa cggtgagcac gattgaagat
      241 aaacgtcagg cattactggc ggggcttggc gtggcgacga tgccgtatcc gatggtcgaa
      301 aaagatattg cggaagggcg gttgcgtgtc gtcagcccgg aatcgaccag cgagatcgat
      361 attattatgg cctggcgtcg tgacagtatg ggggaagcga aatcctggtg tctgcgggaa
      421 attcccaaac tttttaacgg aaaataatat ttattattgt taataaaaag gggcgaaata
      481 tcgccccaat tctaagatta atttgaacca aacttcggat cgtttccgta acgattgctg
      541 ccagaagtac cttctgtgca gaaaaaaacc agaagcacga gccagccaat aaacggaaca
      601 aaaaagagca aggcccaggc acctgaacgg tcggtatcgt gtaagcgacg gatagcaagc
      661 gcaaggacgg gaaggaaggt tgccagcaaa taaagcatag acagatatgg aagctccaga
      721 cctaaaatca attgaatgac attgataata gcgccgacga tggcgttaat cagagtaaac
      781 atccagtact ctttacgacg tgcgcgacca gagaaaccaa cataattttt taatacggat
      841 aaataccact gcataaaaac atccctttaa ttaaaagtga cgaaagtct
//
LOCUS       DAZKFZ010000504          879 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000504 DAZKFZ010000000
VERSION     DAZKFZ010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.508"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..557
                     /locus_tag="ACHOQW_005147"
     CDS             <1..557
                     /locus_tag="ACHOQW_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9576004.1"
                     /translation="VTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMA
                     EDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASF
                     VLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKC
                     MRGCEMRGMVANFGGFLSINKLSQ"
     gene            568..>879
                     /locus_tag="ACHOQW_005148"
     CDS             568..>879
                     /locus_tag="ACHOQW_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9576005.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FR"
BASE COUNT          167 a          218 c          313 g          181 t
ORIGIN      
        1 cggttacctg gcaggggcgg caatatcagg catacccgat tgacggcagt ggctttgaga
       61 tgaacgggaa gggcagcagt gccagaccgt cgctgacggt gtcgaatctg tttggtctgg
      121 tcaccgggat ggcggaggac ctgcagagcc tggtgggggc cacggtggtc cgccgccggg
      181 tgtatgcccg ttttctggat gcggtgaatt ttgtggcagg caatcctgag gcagaccctg
      241 agcaggagct gacggaccgg tgggtggtgg agcagatgtc atcgctgacg gccatgacgg
      301 cctcgtttgt gctggcgaca ccgacggaga cggacggggc gctgtttccc ggtcgcatca
      361 tgctggcgaa cacctgtatg tgggattacc ggggcgatga atgcgggtat aacggtccgg
      421 cagtggcgga tgagttcgac aaccccacca cggatatccg gaaggacaga tgcagtaaat
      481 gcatgcgcgg gtgtgagatg cgcggcatgg tggctaattt tggcggtttc ctttccatca
      541 ataaactttc gcagtaaatc ctgttttatg acacagactg aatcagcgat tctggtgcat
      601 gcccggcggt gtgcgcctgc ggagtcgtgc ggcttcgtga taggcacccc ggagggcgaa
      661 cggtaccagc cctgcgtgaa tatctccgca gagccggagg cgtattttcg tattgcgccg
      721 gaagactggc tgcaggcaga gatgcagggg gagattgtgg cactggtcca cagccacccc
      781 ggtggtctgc cctggctgag cgaggcggac cggcggctgc agataaaaag tgcactgtcc
      841 tggtggctgg tctgccgggg ggaaattcat aaattccgc
//
LOCUS       DAZKFZ010000505          876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000505 DAZKFZ010000000
VERSION     DAZKFZ010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.509"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..395)
                     /gene="escT"
                     /locus_tag="ACHOQW_005149"
     CDS             complement(<1..395)
                     /gene="escT"
                     /locus_tag="ACHOQW_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFP9576006.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFIS"
     gene            complement(395..664)
                     /gene="escS"
                     /locus_tag="ACHOQW_005150"
     CDS             complement(395..664)
                     /gene="escS"
                     /locus_tag="ACHOQW_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFP9576007.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(664..>876)
                     /gene="escR"
                     /locus_tag="ACHOQW_005151"
     CDS             complement(664..>876)
                     /gene="escR"
                     /locus_tag="ACHOQW_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFP9576008.1"
                     /translation="FTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTI
                     SIPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          321 a          165 c          155 g          235 t
ORIGIN      
        1 gagataaatt ggtacaagat aacgccagtg atagaagatg aatcacttat ggacgggtta
       61 aaaattgaag atattgttga gcctcttaga gtatcaataa tctgtccagc cgcatctatt
      121 gcccaaaaaa gaatagtaaa tgataaccca atgaaaaaac caatacttat ttcttttagc
      181 gctattcctg tcaactgaaa gattccagat ggcaattttt ccgaaaatgt atagttctct
      241 actatgatcg gaagcgtaaa cgcaatcata attgagttgc gaataaagtt actgagtaaa
      301 acacctgttg aaaatattgg taggataata aacatcccca gcggcctgag tatacaataa
      361 aatgatgata ctatgaccgt cattatctca ttcattagcc gttcaccttc ggaatcattt
      421 caaatattat cgacgagaag ttgatgattg ttgttcccat ccagtgataa gtcagggcaa
      481 gcgtagcaaa cactgctatt atttttagca aaaaaggcaa tgtttgatcc tgtaactgcg
      541 ttatagcctg gaccagacta ataataatac cgataacaga ggccgctatg actgtaggca
      601 atgagaggat aaatattatc cagaacgttt gcacacataa ttgaacaaaa tatccagtat
      661 ccatcaattc accaccaaca gaaattcgaa taatttttgc cagcctccaa caagaatgaa
      721 taataagatc ttaaaaggaa tcgaaattgt tactggcgat accatcatca tacccaaggc
      781 caataagata ttggaaatga tcaaatctat cgcaataaag ggtaaataga gcaaaaaacc
      841 tatcttgaat gcagcttcaa gctgccccat cgtgaa
//
LOCUS       DAZKFZ010000506          862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000506 DAZKFZ010000000
VERSION     DAZKFZ010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.510"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..300)
                     /locus_tag="ACHOQW_005152"
     CDS             complement(<1..300)
                     /locus_tag="ACHOQW_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFP9576009.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYI"
     gene            complement(297..>862)
                     /locus_tag="ACHOQW_005153"
     CDS             complement(297..>862)
                     /locus_tag="ACHOQW_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9576010.1"
                     /translation="SQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHELFAD
                     ALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQSLIQT
                     SLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTIAHKY
                     GYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          264 a          185 c          144 g          269 t
ORIGIN      
        1 gatataaata gctttgccat catcatacca aataagcccc atctgtgccg acaggcgttc
       61 aatcaaggca tagggttctg ttaaatcaaa atttccgctg atctgtttct tcatggcaag
      121 cttactaact attgtaggct tacccgcata tgatgaaacc gtttcaaaaa aactacgtag
      181 attttcttta tttgctacgt atccattcgc tgctgcggca tatgcaccag gagtcaataa
      241 tccattaaaa atgcataaga ctgaaattaa tataatagta atgcgtaatt ttattttcat
      301 aactttttac atagttctct cggtgttttt cctaaccggc tttttacttc ggctgaaaag
      361 tgtgatgagg acgagtaacc atatttatgc gcaatagttg tcatatcact gtttccttca
      421 atgatttcca acacggctct ggcaactctc cagccacata attccgtttt aactttacca
      481 cccaatgcat aactacataa acggcgaaaa tgtgaatacg atacgccgta aagctcacca
      541 agatcataga gtgaagtctg tatgagcgat tgcgataata aataacgaac cagtttatag
      601 cattctgttt ttcttagatg ctgaaacagg atttcattaa tattatcgct gctcagataa
      661 gtggctaaaa accaggcttc acttgcaact ttactgaata gcacaacttc tgatgcaaag
      721 ggcaaggcat cagcaaacaa ttcatggtgt tccctttcca cactccccgt tgattcatct
      781 ataaaagcta ataatttact caggatatca atgcgaagtg gatgataaat tactttccct
      841 gcagttacct caatttggct tc
//
LOCUS       DAZKFZ010000507          861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000507 DAZKFZ010000000
VERSION     DAZKFZ010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.511"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(149..712)
                     /gene="fimA"
                     /locus_tag="ACHOQW_005154"
     CDS             complement(149..712)
                     /gene="fimA"
                     /locus_tag="ACHOQW_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9576011.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          259 a          198 c          167 g          237 t
ORIGIN      
        1 atattatgtg atttttcata gcgctccaaa tacaaaccac tcatcattaa taatgagatt
       61 cacattggtg aattgctcgg gtgtgcgtag ccatgccaca cacaccctgt tttaatttca
      121 gatgccgtat agccacctta atattgaatt atagatattg aactttgaag gtcgcatctg
      181 cgtttgcagt accagctacg gactgcccga gagcaatata acgagcctgg aatggtagta
      241 tattcgtgcc atcaatcaag tcggttttag cactaaaagt ggccccatca agtattaata
      301 cctcacccgt acggtccaaa atttgaatac cgacattttg ggcagagcct gccgctgaac
      361 tctgcagcgc taacgtatcg tcattactgg tgacagtagt acccaggaat gcaacagctg
      421 cattactgga aacattggta tcacaatcat tcagcttgat attgaagcca acggcggagc
      481 tgaggtcacc cgctttagcc aggcgagaag aacgaacctg acccagctca accgtttggt
      541 tcattgattc ggaatcaatc gcacaagcgg catttaccac ttcaccttta aaatgaataa
      601 caccgccatt aacagtggtc gttactgatt catcaccctc ggctgcgctt accgcagcag
      661 acgacatcgc caacacagaa acgacaatca taccaacatg tttgagtttc ataattctcc
      721 aaaatttata tttaagtagc tgaataaaga gagttaacaa ttaagtcatt ctgttattgc
      781 ttataaaaca agaatcattc ttgattggtt gttgccctga gaatatagtt tccagggtta
      841 ttacatttca cgaagaacga t
//
LOCUS       DAZKFZ010000508          855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000508 DAZKFZ010000000
VERSION     DAZKFZ010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.512"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..94)
                     /locus_tag="ACHOQW_005155"
     CDS             complement(<1..94)
                     /locus_tag="ACHOQW_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9576012.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRTS"
     gene            complement(159..758)
                     /locus_tag="ACHOQW_005156"
     CDS             complement(159..758)
                     /locus_tag="ACHOQW_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9576013.1"
                     /translation="MRKLYTAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDRNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLCVTDSKGKRHDVLTGSDDARHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          199 a          254 c          201 g          201 t
ORIGIN      
        1 tgctggtccg tctggctttc agttgaatgg tgcagttctg tattggtttt cccgcaccat
       61 ctttcagcac gcctgatatt tgtactgctg ccataccctc tccacaaaaa agcccgcctg
      121 aaccggcggg ctgtcataac actgtgttac ctggctaatc agaacttata accgacaccc
      181 acgatgaaac cgtcagtgcg ccagtcacca ctgccggaac cttcataagc gacatcaatg
      241 gccacggatt cggtcgggtt aaactgcacg ccagctcccc acgccagaga cgtgttgctg
      301 tggcgagcgt catcacttcc ggtcagcacg tcgtgccttt tccccttgct gtcagttacg
      361 cagagataat ccccggaaaa agtcgacaca cggctgtaag ccacacccgc catcgcatac
      421 gcgctgaacc attcattcac gcgtacagac ggccccgcca tcacgctgaa ccagcggtta
      481 cgcacggaat cttcatgcca gcgggtatcg ctgtaacggg taatctggcg attcctgtct
      541 cctgcatagc tgaatgacgt caccattccc agagtgtccg taaactcata acggtatttc
      601 acgttaatcc cgttcagttc atcgctgcca ggaacgttcg tcgagacatg aagatacccc
      661 gcgctcagcg tggactgatg ttcagacgcc catgcaggcg caccggatac ggccagacaa
      721 atggctgcgg acaaaatggc ggtataaagt ttacgcataa ttacctctcg cttttctgca
      781 ataaaaaagg cgccatttct ggcgcccgta tatgggttat aaaattcagc tgatactgat
      841 gcctgcggtg gcttt
//
LOCUS       DAZKFZ010000509          847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000509 DAZKFZ010000000
VERSION     DAZKFZ010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.513"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..558)
                     /locus_tag="ACHOQW_005157"
     CDS             complement(<1..558)
                     /locus_tag="ACHOQW_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576014.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQS"
BASE COUNT          227 a          137 c          167 g          316 t
ORIGIN      
        1 gctttgaata tcttttatat tagtcggtaa gtcagctcgt tgttcagcaa taaaattagt
       61 atctgaaaac atggtttgga cactgaatct tactggtatt ccctcttcta cgctaatttt
      121 agttatgatt gccttgtcgg gtttaggatt agcgacaaca atgattcgac attcagcact
      181 tttgttcttt tcgtatctaa tgttattaat ttgattaagt atttcctgtg cctgccgcgt
      241 aagtggtgtc gaacttatcg attcaatgtt agtaataaga cttacaagtt gagccacaac
      301 ttttgagtag tctgcatctg tatatttggt gttcgtgcgg cacgcataaa tttgtgggag
      361 gtgttgccgc gctaaatcat attgttcagg gttgtatcct tctccagaga atgtggaatt
      421 aatgggttgt tgttcttccc gattatcaca aggccacatc ggtgtgtctg gtgttttaac
      481 attgtcggta tctgttgtgc ggttataaat atcgccgtga tatgtattta tcatacctgg
      541 ggtaaagtca attgacataa cattccttat tcattatctg ttttatgcta agatttttaa
      601 attggataat taaagcagga atggattttc ttctacgcac aataaatatc atgcgtcata
      661 tcttcctgat ataattacct ttgacttccc ttttatctcc acccggtaat attcgtgtat
      721 aaaaaagggc attgatagta tcgtaaagga aaacgttaac aactatcaaa aagcgctcat
      781 tttaaaaagt tatgatgttc tccgatagtg tgtgaaatga tatttttgtg atgttttaga
      841 tgtgtaa
//
LOCUS       DAZKFZ010000510          835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000510 DAZKFZ010000000
VERSION     DAZKFZ010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.514"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..538)
                     /gene="fimE"
                     /locus_tag="ACHOQW_005158"
     CDS             complement(<1..538)
                     /gene="fimE"
                     /locus_tag="ACHOQW_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP9576015.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARF"
BASE COUNT          274 a          183 c          165 g          213 t
ORIGIN      
        1 caaaacgagc agcattactg gcggtataac gtacagtatg gcgaatattt cgatgcccga
       61 gataatcctg aattaaacga gtatctgcac cacgctccgc cagttcataa ccgcaagcat
      121 gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg gcatcacgaa
      181 taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata aatatggcgt
      241 cggtccggtc agcgcctttc cagttagcac gttctagggt ccagcgttcc acagcttcac
      301 gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg cgaatattta
      361 ttcggccttc attaaggtca agatcctgat aatgcagatc aagcagttca ctaatacgca
      421 tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt gccccgtaac
      481 aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt ttactcacaa
      541 tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc tcaatcggtc
      601 aaaattagcc caatagataa aaagataacc acacacgaag tgatgtggct atcagtcaat
      661 tacattaaat atcatacaaa aataaaatat cactgatgtg ataacatcta ttttcattga
      721 tttgattcaa tataacaata gattgcagtg gattaacttc cagttgctta aaaaataaag
      781 gaaaaagtgc agaaacagga gagtttaagt gatgtgtaaa tttatgaatg atttt
//
LOCUS       DAZKFZ010000511          831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000511 DAZKFZ010000000
VERSION     DAZKFZ010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.515"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..630
                     /locus_tag="ACHOQW_005159"
     CDS             <1..630
                     /locus_tag="ACHOQW_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9576016.1"
                     /translation="GLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEESS
                     YFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDRN
                     MQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIMG
                     YMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          273 a          143 c          156 g          259 t
ORIGIN      
        1 gggctttgta gtcgttataa aagtcttaca tgcaatagtt gctcaatgca ttgccaaata
       61 atgccagaag agtcaccgcg tttgcagtac tgcgcaaact cgtgtttttg tatgtggacc
      121 gaagaaagtt catattttaa tcgtggtgtg gtagaaggta tcttaacaaa aaaccacaat
      181 gcgagattaa gcggatatat ttttgtcgat ttttcagtaa gttttttacg tctattcctt
      241 gaaaaagact ggattgacta tcttgcaagt actgacatgg ggattgtttt agtcagcgac
      301 cgcaatatgc aatcgttagc taattactgg cgaaaacaca actctgcaat atctgctgtc
      361 atatataatg atgatggtct tgatgttgcc aatgaaaaga tcagacaact atttattggt
      421 cgttatttat catttaccag aggtaacaca ttaactcaga tggaattcac cataatgggg
      481 tatatggttt ctggttataa tccatatcag attgctgaag ttctggatat ggatatccgt
      541 agcatctacg cgtacaagca acgaatcgaa aagagaatgg gtggtaaaat aaacgaatta
      601 tttattcgtt cacattcggt ccaacattga tactcaacaa tctgccagaa tcatactctg
      661 tttatacggg catttattga aatgcccgta ttttataaat aggccgtccc ctcaagagta
      721 aacaccatag tatttaaaac catcacacga aaaattcaga agcattacga aatatgaact
      781 tacaaacaat agtggcataa atgttaacca tgttaattta cataaaattt t
//
LOCUS       DAZKFZ010000512          829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000512 DAZKFZ010000000
VERSION     DAZKFZ010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.516"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..260
                     /locus_tag="ACHOQW_005160"
     CDS             <1..260
                     /locus_tag="ACHOQW_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9576017.1"
                     /translation="KKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENGIIPE
                     QDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            374..>829
                     /locus_tag="ACHOQW_005161"
     CDS             374..>829
                     /locus_tag="ACHOQW_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9576018.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRL"
BASE COUNT          213 a          146 c          231 g          239 t
ORIGIN      
        1 taaaaaaact tatgcagcgt ctgtgtggtt gcggaaagca tgatgaccgt gaacacgggg
       61 agttacttac agcacagctg cgactggggc cggcagacat cctggagtca gatgagaatg
      121 gtattatccc ggagcaggac agggtaatca cgcaggtggt gatactggat gcggataaaa
      181 agcagataca gtgtgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa
      241 atattggcgg gatgaaatag ccgacagctt cacaaaaacc ggagtccggc tccggttttt
      301 tgttgtcacg tatgggggat gtttgttaca gctatttaag tctggagttc aaattaaaat
      361 agggagcttt gttatgccat taacctcaga tattagatca cattcattta atcttggggt
      421 ggaggttgtt cgtgcccgaa ttgtagccaa tgggcgcgga gatattacag tcggtggtga
      481 aactgtcagt attgtgtatg attctactaa tgggcgcttt tcatccagtg gcggtaatgg
      541 cggattgctt tctgagttat tgcttttggg atttaatagt ggtcctcgag cccttggtga
      601 gagaatgcta agtatgcttt cggactcagg tgaagcacaa tcgcaagaga gtattcagaa
      661 caaaatatct caatgtaagt tttctgtttg tccagagaga cttcagtgcc cgcttgaggc
      721 tattcagtgt ccaattacac tggagcagcc tgaaaaaggt atttttgtga agaattcaga
      781 tggttcagat gtatgtactt tatttgatgc cgctgcattt tctcgtttg
//
LOCUS       DAZKFZ010000513          828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000513 DAZKFZ010000000
VERSION     DAZKFZ010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.517"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            48..>828
                     /locus_tag="ACHOQW_005162"
     CDS             48..>828
                     /locus_tag="ACHOQW_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9576019.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSL"
BASE COUNT          271 a          167 c          161 g          229 t
ORIGIN      
        1 atctattttt ctaaacatca gtgaaatact cataaaaggt aactattatg tctatgccat
       61 taagcaatgt ccttcagagt caaattatta cagataatca cttccttcat catccaaaag
      121 tcgaaagtga gttaacacgt aaatatgaac gtgcacgcct ggatacggaa aatatctatc
      181 ttctgcctct tgctcgtggc aataaccata attacgatgg aaaaagcgtt gtcgaaatta
      241 gaaaacttga tattagtaaa gaatcctggc catttaatta tgtgacagaa gcatgcagag
      301 aatccgatgg tattacaaca acaggacgta tgctctatcg aaacttgaaa atcacctcag
      361 ctttagacga aatatatggt ggtatatgta aaaaggcgca tgctgctaca gagttagcag
      421 aaggtttacg gctaaattta tttatgaaga gccctttcga tcctgttgag gactatacag
      481 ttcatgaaat aacattaggc cctggttgca atgttccagg ttacgccgga accacgatag
      541 gatatatttc aacccttcca gcttctcagg caaaaagatg gaccaatgag cagccaagaa
      601 ttgatatcta tattgatcag ataataactg tctcaggggt agcaaattct tccggattcg
      661 cccttgccgc attacttaat gctaatattg agttgggtaa tgacccaata attggtatag
      721 aagcctatcc aggtaccgcg gagatccatg caaaaatggg atataaagtt atccccggtg
      781 atgaagatgc gccactcaaa cgcatgactc tgcaaccgtc atctcttc
//
LOCUS       DAZKFZ010000514          826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000514 DAZKFZ010000000
VERSION     DAZKFZ010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.518"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            46..>826
                     /locus_tag="ACHOQW_005163"
     CDS             46..>826
                     /locus_tag="ACHOQW_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9576020.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLT"
BASE COUNT          271 a          163 c          160 g          232 t
ORIGIN      
        1 aaactatgtg atttccattt cgatttattt agtgtttatt gacgtatgta ctggattatt
       61 aacgataata tcgagttctg gcctgagcac cgaaaattaa tatcggtaca taacgccgat
      121 cttaacgtcg ttctgacaac gccagccagt cgttgtttat cactgctact tgaagctttt
      181 cctgatgtgg ttgcacaaca agattttttc accagagtct gggaagaaga aggtatgcgt
      241 gtgcctacta acacgttata tcagaacata tccattatca gacgcggatt tcgcgctgtt
      301 ggtgatacta cccactcgct tattgcaacc gtgccgagaa gaggattcaa gatccataat
      361 gacattaaca tacaaaatca tgtaataaac tcatcaacag acgcacatac acacaatgcc
      421 ccacctgcca taaaagttaa tgcgggatac aaagagagca ttggtggcgc aaagaatttc
      481 aataacaaaa tcctcaaaca tataaaaagt tatctaatta tgttgagcgc atttgtcata
      541 ggcgcatatt ctgcatattg gctatggaat aataatcaac cgaagccatt tttcaaggat
      601 tacaagactg ttgcggaaat taatggctgc cattttaatg taacagaaga tacaattgat
      661 gggttgaaag agttcgataa atataaaaca cggatactgg attcggggat taactgcaaa
      721 aaatatccgt ggttatactt ccctcttgca aaatcctcac cggggatgat tgttatggca
      781 tgcaacaaaa actataacca acatgaagtg gcaggctgtt taaccc
//
LOCUS       DAZKFZ010000515          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000515 DAZKFZ010000000
VERSION     DAZKFZ010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.519"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(4..570)
                     /locus_tag="ACHOQW_005164"
     CDS             complement(4..570)
                     /locus_tag="ACHOQW_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9576021.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          258 a          186 c          131 g          248 t
ORIGIN      
        1 catttacatt tccttcgata gattcgcata agtgctcctt tcgttgccgg aaaaatcacc
       61 gtaatactta tctcgggctt cttcagcaac tagtaccgct aactccagat catcaaagca
      121 tccgaagtgt ttactcttgc catggaatcc tagcctaaca ttccattttt tctgtcgttt
      181 gtgccaagta acccctctgc aacctgattt gctattcttt cggatcctta tatttcttga
      241 attttctatt ggcaggcatt ctcttaaatt ttctggccta ttgtcggtcc taattccatt
      301 aacgtggtca atttgaccag caggccaacg attatgagtt atgtaaaaaa ctaagacgtg
      361 agttttatat ctacgcccat ctatcatgat cattgaataa ccgttggaat caaaagctcc
      421 agcaacacta tttaatgcta tccttccctg agtgggaact ttccatctaa ataccccggt
      481 agatttatcg aaacttagta actcaaatat ccttttaaca gttaaatctt ctcttttacg
      541 gttacatcgt cttcgcgctg gtttagccat cgccttcttc ctccgtaatg gttttctgaa
      601 tttggccacc tgaacagagc tcaccaaagc tatggatgtc ggtatttcca caataccaag
      661 atggcgaaaa taactgcatg ataagcctca gggaaaaggg aagacactac ccccgataat
      721 tcagaaacaa atcgaaatac atgaactaaa gaaaaaatca caacaaatag aaatagaaat
      781 agaaatagaa atagaaatag aaatagaaat attaaaacag acc
//
LOCUS       DAZKFZ010000516          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000516 DAZKFZ010000000
VERSION     DAZKFZ010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.520"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          284 a          170 c          147 g          222 t
ORIGIN      
        1 ctttataaga tagatctcat tgcataaaca aataaaacag aataaaaacc aagctgaacc
       61 gtttcactat actctactta attcgagtta caggaaggca gcaagcgagt gaatccccag
      121 gagcttacat aagtaagtga ttggggtaag cgaacgcagt cgcagcacat gcaacttgaa
      181 gtatgacgag tatatatatc tattagctgt ttcccggaca taagctacag ccagcactaa
      241 cctggaacgg gtgcattata aaaaggaaca agggacaaat agctgaacag taaccgcgtt
      301 agtctcttcc cgcataaccg taccattcgt attaagaagt attgcccata tccattcggc
      361 gaaagaggat ctgccataca tcaagtccgt tttacttggc atcagatcat cgccgtcatt
      421 aactctgtta aagtcggaca aacgattagc aagatctgtg attcaaaagt gcttagcgct
      481 aaggtgggac tggttacaga ttcattaacc ctgttctttg tcgcacctat cgatcggtgt
      541 aatgccacaa caggtttatg ttaatcagaa caatgcgccc ctctataaag agaattcttt
      601 gataaggcaa cttgaaaagg gaaagctaac tgatatcaat gtaccacttt agtaatcatc
      661 acattatata caggccaaaa taagtttcct gaaagccagt atctctaaat caatcattaa
      721 tgatatattt tatcgtcaca ataaaaaatt atctaaattc tattacttat gtaaataagt
      781 acaacctttt catagacaca aaaactatgg ctgacgcatc act
//
LOCUS       DAZKFZ010000517          823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000517 DAZKFZ010000000
VERSION     DAZKFZ010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.521"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..384
                     /locus_tag="ACHOQW_005165"
     CDS             <1..384
                     /locus_tag="ACHOQW_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9576022.1"
                     /translation="SSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNL
                     KLGSFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTD
                     ITRGATYPVLIDSIAVEVNSTDTAA"
     gene            521..700
                     /locus_tag="ACHOQW_005166"
     CDS             521..700
                     /locus_tag="ACHOQW_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9576023.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            627..>823
                     /locus_tag="ACHOQW_005167"
     CDS             627..>823
                     /locus_tag="ACHOQW_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9576024.1"
                     /translation="MTKGPQRKSAPVSQPEANPKSRSFSGNFLNHIFRRENPMSEITS
                     LVTAEAVKEVLRSEEVRSALK"
BASE COUNT          232 a          191 c          221 g          179 t
ORIGIN      
        1 tcttcactgc cacagggcgt tgccctgacc ggagacgggg ggaataacct gctggcagcg
       61 ttctacatcc agacagatgc aaaagacctg aatgtgatgt accacaatgc gaaagtggcg
      121 acaaacaacc tgaaactggg aagctttggc gcatttgata acgaatggca tacgctggct
      181 ttccgctttg ccgggaataa cagccttcag gtgacgccgg ttattgatgg tcaggatggc
      241 acaccgttca cgctgacgca gtcaccggtc agtgcctttg cggcggataa actgcatgtg
      301 acagacatta ccagaggtgc gacttacccg gtactgatag acagcattgc ggtggaagtg
      361 aacagcacag acactgcggc atgataaaaa aaaccgccag cgacaggaat ggacgctggc
      421 ggtggtgata cctatggaga aaaaataaag gaacgatact ttcgtactct ggttttttaa
      481 tgaaaacagt tcttattgtc aacaataacg gaaagaaatt atgacatttc tgaaccagtt
      541 aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg ggtacagggc
      601 agtgcgcgat tgctggcgca ggcagattga caaaagggcc gcagagaaaa tcagcgccag
      661 tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag cggcaacttt cttaatcaca
      721 tctttcgacg agaaaatccc atgtcagaaa ttacatccct ggtcactgct gaagcagtga
      781 aggaagtcct gcgctctgaa gaagtccgga gcgcactgaa aca
//
LOCUS       DAZKFZ010000518          816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000518 DAZKFZ010000000
VERSION     DAZKFZ010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.522"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..755
                     /gene="ydeO"
                     /locus_tag="ACHOQW_005168"
     CDS             <1..755
                     /gene="ydeO"
                     /locus_tag="ACHOQW_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP9576025.1"
                     /translation="VCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERH
                     FKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHN
                     FSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYIS
                     ESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRK
                     HFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          254 a          159 c          160 g          243 t
ORIGIN      
        1 tcgtttgttc tgttatattt attcatcatg ccttcaacgc taacatttta gataaagatt
       61 acgccttctc tgacggcgag atcctgatgg tagataacgc tgttcgtacg cattttgaac
      121 cttatgagcg gcattttaaa gaaatcggat ttaatgaaaa taccattaaa aaatatctac
      181 aatgcactaa catccagaca gtgacgatgc ctgttcctgc gaagttttta cgtgcttcga
      241 atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa gaacgcaatc
      301 atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc acatgcaaag
      361 gtttcattac actattaact aacggtgtgc tatccgtttc ggggaaagtg agaaatattg
      421 tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc ctgtacatca
      481 gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca cagattcttt
      541 tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca gtcaataaaa
      601 ttgccgaaca atgtggttat gccagtacat cttattttat ttatgcgttc cgcaaacatt
      661 tcggcaacag tccaaagaga gtttctaagg agtaccgttg tcaaaggcac acgggtatga
      721 atacgggcaa cacgatgaat gctttagcta tttgattatt tgctaacgag tagtcaacca
      781 cgcaccctac gtaaaaatta aaaatggggc agtcat
//
LOCUS       DAZKFZ010000519          810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000519 DAZKFZ010000000
VERSION     DAZKFZ010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.523"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..428
                     /locus_tag="ACHOQW_005169"
     CDS             <1..428
                     /locus_tag="ACHOQW_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9576026.1"
                     /translation="KSTVSVFYLQPEEVNTLSHQAKRGDGEAGFRLFLYYKLSNYDEE
                     KSSQWLKIAANNGHATAQYRLYVELQEQGETKEANEWLQKIKLAASNNDSSAIWFLVN
                     CYKNQGNKKEALQWAYKLKASKQPLDADRLIEELTKEQK"
BASE COUNT          296 a          137 c          170 g          207 t
ORIGIN      
        1 caaaatcaac agtaagcgtc ttttatttgc agccagaaga agtaaatact ttatcacacc
       61 aggcaaaacg aggcgatggc gaggcgggtt ttaggttgtt cttgtactac aagttgtcca
      121 attatgatga agaaaaaagt agtcagtggt taaaaatagc ggctaataat ggtcatgcga
      181 cagctcagta tcggttatat gtagagttac aagaacaagg tgaaaccaag gaagctaatg
      241 aatggttaca aaaaattaaa ttggctgcca gtaataatga cagttcagca atatggttct
      301 tggtgaattg ttataaaaac cagggaaata aaaaagaagc attgcaatgg gcatataaac
      361 tcaaagcgag taaacagccg cttgatgcag atcgactaat tgaagaatta acgaaagaac
      421 aaaagtagct ttaaacaggt aacagaaaaa tggagagtag aaaaacttta ccagtcgcta
      481 caacacctac acacaaaatt acgtttacga cgactgcaac aagctcatca aagcgcaagt
      541 tgaaaccaaa actggcatct tcgtcgccgg ttatcattac gatgtacttg gcagatgcat
      601 caaaagtcgc aagagaaaag atgaaaatat agctttttgt atcattaagt atgtggccag
      661 cacatgactc ggcatagatc ttatgactaa gattctacac cccaaaccta agtatgggcc
      721 aaagaagccg tttggttcat tttttatatc tccagcattt tagtggatgg agcttatgaa
      781 aataataagg ttattattgc tggcttgttc
//
LOCUS       DAZKFZ010000520          807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000520 DAZKFZ010000000
VERSION     DAZKFZ010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.524"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..161)
                     /locus_tag="ACHOQW_005170"
     CDS             complement(<1..161)
                     /locus_tag="ACHOQW_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9576027.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALA"
     gene            complement(142..555)
                     /locus_tag="ACHOQW_005171"
     CDS             complement(142..555)
                     /locus_tag="ACHOQW_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9576028.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(582..>807)
                     /locus_tag="ACHOQW_005172"
     CDS             complement(582..>807)
                     /locus_tag="ACHOQW_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9576029.1"
                     /translation="SHSLKGTLASPETEMQQIRREVMLGWPADALNQATNRVLYLSGM
                     LDNRHDADPEQTGKAEATESVTSKKHSKAS"
BASE COUNT          179 a          256 c          191 g          181 t
ORIGIN      
        1 gccagcgcct gtcggctcag cccctgttca acagtactgg cggtttttct ggcatccgcc
       61 tccagactgg aaaaatgacg gcgtacccgg gccatctgtt catcaaaccg ggccgcatcc
      121 agactcaaat caataaccag atcacccgct ggctgggaca tatctcacac ctccggaaat
      181 ccccgctgaa gccatcatta atgcaacatc atcctcgctg acatccacca catccgcaga
      241 aggtgaaata tcgcgccctc cgtcccctcc gaaccggacg cctccggcaa ctcctgtcgc
      301 tttctgcatc agcatttctt cctcgtccgg catctccgtc tgctcttcct cacacgctgg
      361 agcaagcaga ctgaaatccg ccggatgcat atccggatcg ccaaaaaaca ggctgagtac
      421 ggcgtacatc agcccggaaa aatgagcgtc cagttgggta tcctgaaaat aatgcgtgca
      481 gtaaaaacgt cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca
      541 gtcgggtctc cccatctctc gcgccagttt caggacaaag ttcagctcgc cttcgaatgc
      601 ttttttgatg ttaccgactc agtcgcttct gctttaccgg tttgttcagg atcggcatcg
      661 tgccggttat ccagcatacc tgaaagataa agcacccggt tcgttgcctg attcagtgca
      721 tcagcaggcc atcccagcat cacttcacgg cggatctgct gcatctctgt ctccggagag
      781 gccagagtgc ctttgaggga atgggaa
//
LOCUS       DAZKFZ010000521          806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000521 DAZKFZ010000000
VERSION     DAZKFZ010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.525"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            29..184
                     /locus_tag="ACHOQW_005173"
     CDS             29..184
                     /locus_tag="ACHOQW_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9576030.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            186..314
                     /gene="ydfB"
                     /locus_tag="ACHOQW_005174"
     CDS             186..314
                     /gene="ydfB"
                     /locus_tag="ACHOQW_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFP9576031.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            326..715
                     /locus_tag="ACHOQW_005175"
     CDS             326..715
                     /locus_tag="ACHOQW_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576032.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
BASE COUNT          263 a          166 c          186 g          191 t
ORIGIN      
        1 cccggcgtta aggggaaatg aggtcaacat ggatactatc gatcttggca acaacgaatc
       61 tctggtgtac ggcgtgtttc ccaaccagga cggtacattc accgcgatga cgtataccaa
      121 aagcaaaacg tttaaaaccg aaaatggtgc ccgtcgctgg ctggaaagaa actcaggtga
      181 gtgatatgga tttcgacaca atcatggaaa aggcttacga agaatacttc gaaggccttg
      241 ccgaaggcga ggaagctctc agcttcagtg agtttaaaca ggcgctttcc agttcggcaa
      301 aatctaacga ctaacggagt taaagatgga atttaaagat ttactaaaag aaatccagga
      361 aattgcagca catgcacttc accaacgtct gaacgaagtt gaattagaat ctgcaacgaa
      421 gaaatacatt gataatatgg ctcgtaatgt gcgcgatgcg tttaccggat tgtactctgt
      481 ttcggtaaca aacaaccaga atactgaaga aactgcaaag cggattgcct cggtgatggg
      541 ttttcatgtc gaggaaaagt attcaaaaaa agaattctgg aaaactacaa aaaaattgca
      601 gagcgagaac tgtcatctcc tgcggcaatc gcttctttct atgaggaaag ttattcagat
      661 gacacaggat taccggaact gctctcatta cttgaaaaat tcggaattat catgaaaggg
      721 actgcgtcgg cggatttcac atactcaatg atggctgctg atggagagcc ttttcctccc
      781 gaacttaaaa taacccccgg caatca
//
LOCUS       DAZKFZ010000522          806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000522 DAZKFZ010000000
VERSION     DAZKFZ010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.526"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            85..654
                     /locus_tag="ACHOQW_005176"
     CDS             85..654
                     /locus_tag="ACHOQW_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9576033.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            654..800
                     /locus_tag="ACHOQW_005177"
     CDS             654..800
                     /locus_tag="ACHOQW_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576034.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
BASE COUNT          219 a          161 c          217 g          209 t
ORIGIN      
        1 ttgaggatac cggtttggct gtcggtgcct ttctgtgggc tggattcctg tgagacaagg
       61 ttcatcacta aaaggaaata accgatgaat atgatggccg tgccgtttca cggcaactct
      121 ctttatgtag ttaaccataa tggcgaacca tacgttccca tgaaacctgt cgttgcgggg
      181 atggggctgg cctggcaatc acagttggct aagttaagac agcgttttgc gtcaactata
      241 acggaaatcg ttatggttgc tgaggatggg aaacaacgca atatggtgtc catgccactt
      301 cgaaaacttg ccggctggct acaaaccatt aatcccaaca aagtaaaacc cgaaatccgc
      361 gataaggtca tccggtatca ggaagagtgc gacgatgttc tttacgagta ctggacgaag
      421 ggttttgtcg ttaatccccg taaaatgagc gtgatggaag aactcaacca ggcttgtgct
      481 gacatgaaac gggataaaaa cattgccagt gtgtttgcta ccgggctgaa tgagtggaaa
      541 caggttaaag ccgcgcatgt atcaaaaatc cgtacgctgg taaatgaagc gaatatgctg
      601 attgattttg tcctggctga tacaggcaaa gggaaaataa caaaggcgga ttgatggggt
      661 ggctaatgat atcagataaa ctcataacgc tggtgaagag cctctgtgta cttgtcggca
      721 tttcattttt agtcatgctg gttgccattt tcttttccac cgcctggcga gtcctgacgt
      781 tatcgggact ggtggggtga aagaga
//
LOCUS       DAZKFZ010000523          804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000523 DAZKFZ010000000
VERSION     DAZKFZ010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.527"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            137..703
                     /locus_tag="ACHOQW_005178"
     CDS             137..703
                     /locus_tag="ACHOQW_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9576035.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
BASE COUNT          242 a          181 c          180 g          201 t
ORIGIN      
        1 gaatctccac aatctcttca ttatcagaat atgcaccatg aataagataa aaatactttc
       61 cattactttg gagaaacgta taatcagcaa aaatgagaac gatcattctc accaagtgat
      121 aggccaggag acagaaatga ccagcaagct ggaaatacgc cacaaacagc gtcaggatga
      181 aatcattaac gccgcccgtc ggtgttttcg tcgctgcggg tttcatgccg ccagtatgtc
      241 gcaaattgcc agcgaagctc agcttagcgt gggtcagata tatcgttact tcgccaacaa
      301 agacgccatt attgaagaga tggttcgccg tatcatcgac tttcgtattg cccaaatgga
      361 cattgacgcg cgtaccgatc acttcccgga agtccttgcc ttgcgtaaat ccttaaatga
      421 ggacgacgac gcgctgatgc tggaagtggc tgcagaagcc acacgcaacc cgcgggttat
      481 ggcaatgctt gaagaagctg acgccagaat gttcgctaac ggttgtgcac atatgaaaag
      541 aatgcatccc catcttagcg aggagcacat tcgttgctgc gttgaagtgt ttgctaccat
      601 gatggaaggt acggtttatc gtcgtttaac accgcaaaaa agcgatccac aacatctgca
      661 ggaaatatat caggatattg ttagtatgtt gataaataaa taagaacaga attaaccaat
      721 agcgggggat attaatcccc cttatttcat tgcgaaattc atttcgcgat gcgggttcgt
      781 tataactgaa tatccctgac atat
//
LOCUS       DAZKFZ010000524          801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000524 DAZKFZ010000000
VERSION     DAZKFZ010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.528"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            15..581
                     /locus_tag="ACHOQW_005179"
     CDS             15..581
                     /locus_tag="ACHOQW_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9576036.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            640..>801
                     /locus_tag="ACHOQW_005180"
     CDS             640..>801
                     /locus_tag="ACHOQW_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576037.1"
                     /translation="MNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISLLNQ
                     KGVVAPGDYF"
BASE COUNT          217 a          178 c          186 g          220 t
ORIGIN      
        1 tgaaggattc atgtatgttt aaaggacaaa aaacactggc cgccctggcc atatctctac
       61 tattcgcttc acctgttttt gctgcagatg aaggatctgg tgaaatccac tttaaaggtg
      121 aagttattga agcaccatgt gaaattcacc ctgatgacct tgatatgaac attgacctgg
      181 gtgaagtaac cacatcccac ataaatcgag aacatcatag cgagaaaaaa cctgtcaata
      241 ttcgcttaat caactgtgat attgcaggct gggatgatgg caagggtggc gtggtctcaa
      301 aggtaggcgt gaccttcgac agcacggcta aaacgacagg tgcaaccccg ctccttagta
      361 acatcagtgc tggcgaggct accggcgttg gcgtacgtct catgaacaaa gatgaatcat
      421 ttgttacatt gggaacagaa gcgccaacaa ttgacttatt ggcaagttca acagaacaaa
      481 cgttaaactt ctttgcctgg atggaacaga tggataacgc agtttcggtt accgcggggg
      541 ccgttactgc taacgcaact tacgttctgg attaccaata atccttttta aggcggctga
      601 ttagccgcct tttgcgaata ttgttcaggt atggatgccg tgaatattta tcgactctct
      661 gttctttcct gtctggcaat ggtaacccct cctgcgctga cagctgaatt taaccttaac
      721 gttctcgata agtcgattcg ggatagcgtt gatatttcgt tgctcaatca aaaaggcgtc
      781 gtagctcccg gcgattactt t
//
LOCUS       DAZKFZ010000525          800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000525 DAZKFZ010000000
VERSION     DAZKFZ010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.529"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..509)
                     /locus_tag="ACHOQW_005181"
     CDS             complement(<1..509)
                     /locus_tag="ACHOQW_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9576038.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQA"
     gene            complement(563..>800)
                     /locus_tag="ACHOQW_005182"
     CDS             complement(563..>800)
                     /locus_tag="ACHOQW_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFP9576039.1"
                     /translation="RSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLIN
                     HTRDVDINEPDGPTNLSAWMRLKNKELPPSPHHY"
BASE COUNT          185 a          231 c          199 g          185 t
ORIGIN      
        1 gcctgtgcag aggacagggg cacccggtaa aagccggtga ccgccatatt gtccaggtaa
       61 aggttctgac cgttgcgcca ccagtcatcc tcacgctcaa aatccggcat atcaattccc
      121 gccagatggt aggcatcccg gaacagcgtg taacagtccg tcaccccgtg ctcaaagcgc
      181 cgtcctgtca gatgtggcac acagcggaat ttatgaattt ccccccggca gaccagccac
      241 caggacagtg cactttttat ctgcagccgc cggtcggcct cgctcagcca gggcagacca
      301 ccggggtggc tgtggaccag tgccacaatc tccccctgca tttctgcccg cagccagtcc
      361 tccggcgaca tccggaaata atcttccggc tcaccggaga tattcacgca gggaaaatat
      421 ctttcccctt ccggcgttct caccacgaag ccgcacgact ccgctggtgc acatcgccgg
      481 gcgtgcgcca atatattgct atagagcatg agaactcctg ataaaaaccc agccgaagct
      541 gggtcatttc gttggcaatc tgttagtagt gatgcggtga aggaggtaat tctttattct
      601 taagtctcat ccatgcggaa agattcgttg gtccgtctgg ctcattgata tcaacatctc
      661 gtgtgtgatt aattaaaacg tctctcgcca ttccaataac atacgagaac tcatgaccgt
      721 agtcgtagca tctgccggaa tagttcgatt gaatttgttt tagcgccgga tacagttcgc
      781 ggaataatgc ctgtgagcgg
//
LOCUS       DAZKFZ010000526          798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000526 DAZKFZ010000000
VERSION     DAZKFZ010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.530"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>798
                     /locus_tag="ACHOQW_005183"
     CDS             <1..>798
                     /locus_tag="ACHOQW_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9576040.1"
                     /translation="AVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAW
                     IGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGVGALFGEL
                     AEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGVSKGPGQQEAVSRL
                     AEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWK
                     AESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEY"
BASE COUNT          217 a          175 c          263 g          143 t
ORIGIN      
        1 tgcggtgacg gaggcgatgt ccctgtttgc ggatgtcatg aatgcacgtc tggagcaact
       61 tgataaagcg gccacgccgg tggaaaaaat ctgggacgat gttaaaacct ggacttctga
      121 cgcatgggca tggataggtg atcatacact gggggcactc agtctgatca ctgacgtggt
      181 ggccggaacc gttgaacagg tgaagctgct gcttgtgcag ggggatctgg cgctggctga
      241 atttattcag tcagcctggg aaacgacaaa gaatgtgccc ggcgttggtg cgttgtttgg
      301 tgaactggca gaagagaacc gcgtatttat tgagaaaaca aaacgcgatg aactggcgct
      361 gagaaaatcc attgcggaac gggatgcgcg tatacgccag ggggaaatgg ggtacatcaa
      421 ccgctcgcgt gcaacaggcg tcagcaaagg tccggggcag caggaagccg tcagccgtct
      481 ggctgaagag ctgacaggta aaaagcatac atcaccgaaa acgcgctctg ccggggagag
      541 ggaagaggag caggcaagag aggctctgct tgcccttgaa gctgagctca ggacgctgga
      601 aaaacacagc ggtgcgaatg agaaaatcag ccggcagcgc cgtgatttat ggaaggcgga
      661 aagtcagtat gcggtcctga aagaggctgc cacgaaacga cagttatctg agcaggaaaa
      721 atccctgctg gcgcataaag acgagacgct ggagtacaaa cgccagctgg ctgagctggg
      781 cgacaaggtt gaatacca
//
LOCUS       DAZKFZ010000527          784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000527 DAZKFZ010000000
VERSION     DAZKFZ010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.531"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            142..744
                     /gene="lpfA2"
                     /locus_tag="ACHOQW_005184"
     CDS             142..744
                     /gene="lpfA2"
                     /locus_tag="ACHOQW_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFP9576041.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
BASE COUNT          236 a          154 c          168 g          226 t
ORIGIN      
        1 taaatgtata aaataaaaac cacttgcgcc aaagtatatt ggtagtacta tttggtttct
       61 gatcaaatac cgatcaggtt tatccaaatt taataaatgg ataacacaca ggaataatat
      121 caaattaatg gagttatatt tatgaaacca aatatgattg taggagcatt agcgttaact
      181 tctgtgttta tggcaggtca cctacaggcg gctgatggaa cagtccattt ccgtggtgaa
      241 attattgaca gtacttgcga agtcactcct gaaactaaag atcaggtcgt tgatttaggc
      301 aaagtaaacc gtacagcctt tagtggcgtc gatgatgtgg ctgccccgac ggctttttct
      361 atcgatctga ctcaatgccc ggaaaccttt aagtccgccg caattcgttt cgatggtaat
      421 gaagatgctc atggtaatgg caacctggca attggtaccc cgctggataa ctctaacgat
      481 gctgccgctg gtattagccc gagtgataac agtggggatt atactggtgc gggtgccgtt
      541 agtgcagcga aaggcgtagc tattcgttta tataaccgtg cagataacac tcaggtcaag
      601 ttatatgaaa attctgcatc aactccgatt tctaatggta atgcatccat gaagttcatg
      661 gctcgttata ttgctacgga aacgactatt gaccctggta cagctaacgc cgactcgcag
      721 tttacagttg aatatataaa ataaatcatt cttaacataa taccttaaat aaaatggcgc
      781 tcgc
//
LOCUS       DAZKFZ010000528          770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000528 DAZKFZ010000000
VERSION     DAZKFZ010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.532"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..145
                     /locus_tag="ACHOQW_005185"
     CDS             <1..145
                     /locus_tag="ACHOQW_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576042.1"
                     /translation="IINTDNKTCDIYFMNGIVFKHYPLMKVISAQQAISALKYMVDGE
                     VYF"
     gene            552..650
                     /locus_tag="ACHOQW_005186"
     CDS             552..650
                     /locus_tag="ACHOQW_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576043.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            647..>770
                     /locus_tag="ACHOQW_005187"
     CDS             647..>770
                     /locus_tag="ACHOQW_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576044.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRE"
BASE COUNT          232 a          163 c          156 g          219 t
ORIGIN      
        1 cataattaat acggataata aaacctgtga tatctatttt atgaatggca ttgtttttaa
       61 acactatcct ttaatgaaag taatatccgc ccagcaggcg ataagtgctc tcaaatatat
      121 ggttgatggt gaggtttatt tttgagtaat aatcactttt tcaaccgtgc tatggtatga
      181 aagtaaagta actactatga tattaactat cgagcaagaa tatccccagg cactcaacag
      241 ggcgcaggtc aacgtgaatc ggtcatgaca cgacaccgat tttgcggaat caatgttgct
      301 tatgaataca acatcccgct tagtacatgt tcaacacatc agcaaatttt gaactgggtg
      361 tggcacttaa ctgaaaaaac atggatgaca caaaatgtta ctcgtcgctt cattgaagta
      421 gcttgtggat atcacaagct agactatcgc caatgatatt caatttttga ttttttagta
      481 tcaaccttcc ataataatgt caccggagct tgaacaactc cggtgacttc tgcgcattta
      541 aggggactta aatgcgacca caatctgaac tccttacctt gtcacagatg cagaaatgca
      601 cctgcgatgt cttgcatcca gcgtttgatc tctgcggagg tgaagcgtga acctcccaca
      661 agatggcatc aaattacatc gcggtaactt caccgctgtc ggtcagcaga tccagactta
      721 tctggaagac ggcaaatgct ttcgcatggt gcttaaaccg tggcgcgaga
//
LOCUS       DAZKFZ010000529          770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000529 DAZKFZ010000000
VERSION     DAZKFZ010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.533"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>770
                     /gene="espX6"
                     /locus_tag="ACHOQW_005188"
     CDS             <1..>770
                     /gene="espX6"
                     /locus_tag="ACHOQW_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFP9576045.1"
                     /translation="NKTETFSFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKN
                     TISTSEIIECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKSI
                     TGDKSERLTRKLSISDAGITMTFKIGDVVINTISTAIPEDATGQRCIEGLNLAEMDLT
                     DIDLSKMALRNVNFNGSILRNAKFSGTICEGVDFTDCDLRNAEFENASLENNDFRKVR
                     HLTYVNFKNANLRNSNFNGKVLTGVTFTGSDLSNAYLE"
BASE COUNT          260 a          149 c          152 g          209 t
ORIGIN      
        1 taacaagact gaaactttct cgtttacaaa cttacaatgg gaaaatttgc tgcaatacta
       61 caaagaaaac caccagcagc caaatatagc aggatcacga aatctcacgg ataatataga
      121 taaaattaaa aatacaatat ccacctctga aattattgaa tgcgcctctc ctgaaataag
      181 aagtagcgtc ctgaacgatc tttatagcat tgctaatttc ctcccggaca ataatctgac
      241 cccaaatgag agctggaaaa gattttgtga gacatgcgag cgcttttacg ttgctcagaa
      301 gagtatcact ggagataaga gtgaacgtct tacgcgaaaa ctctctatct ctgatgcagg
      361 aattacaatg accttcaaga taggtgatgt tgtcatcaat actattagca ctgctattcc
      421 tgaagatgca acgggtcaac ggtgtatcga agggttgaat ttagcagaga tggatttaac
      481 cgacatagac ttgtcgaaaa tggcgctaag gaatgtcaat tttaatggca gcattcttag
      541 aaatgccaag ttctccggta cgatctgtga aggcgtggat tttaccgatt gtgatctgcg
      601 taatgcagaa ttcgaaaatg cctcattaga aaataatgat tttcgtaaag ttcgccactt
      661 gacttatgta aatttcaaaa acgcaaatct acgaaacagt aacttcaacg gaaaagttct
      721 cactggcgta acctttactg gaagtgacct tagtaacgcg tatcttgaac
//
LOCUS       DAZKFZ010000530          765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000530 DAZKFZ010000000
VERSION     DAZKFZ010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.534"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            61..635
                     /locus_tag="ACHOQW_005189"
                     /pseudo
     CDS             61..635
                     /locus_tag="ACHOQW_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
BASE COUNT          224 a          124 c          177 g          240 t
ORIGIN      
        1 ggcagatgtt tgttaaagct atttaagtct ggagtttaaa ttaaaatagg gagttttata
       61 atgccgttaa attcggagat tagatcaagc tcatttttaa tggattgaat gtccttcgag
      121 ctcaagtagc atctagcggt cgaggggagt ttacattagg taatgagact gtcagcattg
      181 tatttaatga aaccgatggg cgttttctat ccagcggcag tagtggggga ttgcttactg
      241 agttattcct ttatgggttt aataacggcc ctgaagctct tcgcgatagg atgctcagta
      301 tgctttcgga ctcaggtgaa gcacaatcgc aagagagtat tcaggacaaa atatctcaat
      361 gtaagtttcc tgttagttca ggaaatttcc agtgcccgcc agagtctatt cagtgtccaa
      421 ttacactaga gagacccgaa gaaggagtgt ttgtcaaaaa ttcagatagt tcggcagtat
      481 gctgcttatt tgattttgat gcattttctc gtttagctag tgaaggctca tatcatccac
      541 tgacccgaga accaataacg gcatcaatga ttataagtcc tgataaatgt gtttatgatc
      601 ctatcaaggg aaacttcatt ataaaagata gttaaaatat ttttctctga gagagtattg
      661 gctgtcagta atttgtaaaa aaacgccgca gacatccaga atataagaag gtgtctgtgg
      721 ttggcaggtt tcagatgact tgaaggcgtc tttatgtaaa tgcat
//
LOCUS       DAZKFZ010000531          754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000531 DAZKFZ010000000
VERSION     DAZKFZ010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.535"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..747
                     /locus_tag="ACHOQW_005190"
     CDS             <1..747
                     /locus_tag="ACHOQW_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFP9576046.1"
                     /translation="DWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVA
                     FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTER
                     LKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNN
                     RRLLGRLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          186 a          170 c          233 g          165 t
ORIGIN      
        1 gactggctga agagagagat acagcgcgta tacgatgaaa atcatcaggt gtacggtgtg
       61 cgtaaagtct ggcgtcagtt gttacgggaa ggaatcaggg tggccagatg tacagtggca
      121 cgtctcatgg cggttatggg acttgccggt gttctccggg gtaaaaaggt ccgtacgacc
      181 atcagccgga aagccgttgc cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga
      241 cctgaccagc tgtgggtggc tgattttact tacgtcagca catggcaggg cttcgtctat
      301 gtggcgttta tcattgatgt gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtct
      361 atggaaacga cattcgtgct ggatgcgctg gagcaggcgt tgtgggcccg tcgtccgtct
      421 ggcaccatcc atcacagcga taaaggctct cagtatgtgt cactggccta tacggagcga
      481 ctaaaagaag ccggattact ggcatcaaca gggagtacag gcgactcgta tgacaacgcg
      541 atggctgaga gcatcaatgg tctttacaaa gcggaggtaa tacaccgtaa gagctggaaa
      601 aaccgtgcag aagtggaact ggccacacta acgtgggtgg actggtataa caatcgacga
      661 ttgctgggaa ggctgggcca tactcctccg gcagaagcag aaaaagctta ttatgcttcc
      721 atcggaaacg atgatctggc agcctgagtt caca
//
LOCUS       DAZKFZ010000532          744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000532 DAZKFZ010000000
VERSION     DAZKFZ010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.536"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            186..261
                     /locus_tag="ACHOQW_005191"
     tRNA            186..261
                     /locus_tag="ACHOQW_005191"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Ile,seq:cat)
BASE COUNT          209 a          138 c          176 g          221 t
ORIGIN      
        1 tgctaattat taacatttgc ttgttgactg cgagtatcga ctttgcggaa tggaggtggt
       61 gtcgggtttt atgtcgcatg tattgacgac ccaggatgga tgagaacgca taaaaatctg
      121 tgatttacat cataagatca tcatgtttat tgcgtgggtt ataagtattg ttattgcata
      181 cttaaggccc tttagctcag tggttagagc aggcgactca taatcgtccg gtcgctggtt
      241 caagtccagc aagggccacc aaatcatcat tcatgtatca ggaagagtta agggtacaag
      301 ggttactcga tggcggtcta tatttcacga ttaacgccat ttcaaggaag aagtgcggtg
      361 ggtggtttcg aatgagggct ggtgttttgg tacgtggcac gccactgcaa tgtatttatc
      421 tatcagtact cattcaaaag tatttggaaa aaatgcctgc tatgccaagc gggcaaattg
      481 cttacatgtg gaattaagta attaacgata aagccagcgt attatcattt attataccca
      541 ttcatttaat caggttaatt actcaaatca ctgaactatt ctctgttcgc tcactgatga
      601 taagggagta ccacaaccaa tgtatgtaga acaatgccgt agagtaagga tgaaatgtct
      661 ctgccacgcc ttaaacatct actactgact agaacacccg ccgcaggtag atgagttttg
      721 tgagcgttac aggagcgtgg ctat
//
LOCUS       DAZKFZ010000533          739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000533 DAZKFZ010000000
VERSION     DAZKFZ010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.537"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(205..711)
                     /gene="espH"
                     /locus_tag="ACHOQW_005192"
     CDS             complement(205..711)
                     /gene="espH"
                     /locus_tag="ACHOQW_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFP9576047.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
BASE COUNT          219 a          158 c          136 g          226 t
ORIGIN      
        1 accttgataa tttctgcgct ggatgggatc atccattccg tttaacatac tcttcatcac
       61 agggacctct gatttctggc cgctaacgtc tctgaagatt cagcctgtat tatctaaaag
      121 caatgtaata tcagttaaaa agcggaaaaa ctccagaatt ataactattc tggagttaag
      181 tcaggaagaa aactcaaatg ctaatcataa tacgctataa gaggaagctc ttgttgctat
      241 agccctatta tcaagacgtt cggtagactg tacacgacgt aacaaagaag ataatcctct
      301 ttcagctaag atcccctcca ggggagtacg aggaaaccag gttgaagaaa gtggagttat
      361 atgtctctca tttaattttc tttccaaagc cttagtcaca ccgccagaaa acacgctaaa
      421 tagcgttgag aaacatccat tttccggtgt tattctttgt actttgtaca cataatcatc
      481 ttttgggcca tctttaataa agtgaacagc atagtctttg cctctaaaag agacaatggt
      541 ggaatcccct atttctggat atacccgatg atggcgttgt tgcattcggg ctttaagctg
      601 aatttcatct acgatatctt cattgggtat atgaagcctt ctgttgaacc tgttccatga
      661 cgcatgttca cttgtcagaa atgtcttgaa taccgctcct gataacgaca tgatacatct
      721 ccctatataa cgtattcct
//
LOCUS       DAZKFZ010000534          726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000534 DAZKFZ010000000
VERSION     DAZKFZ010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.538"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..599
                     /gene="nleB"
                     /locus_tag="ACHOQW_005193"
     CDS             <1..599
                     /gene="nleB"
                     /locus_tag="ACHOQW_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP9576048.1"
                     /translation="SDIYLENIHKQESYPASERDNYLLGLLREELKNIPEGKDSLIES
                     YAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVL
                     YAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGI
                     KRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTCSSW"
     gene            648..>726
                     /locus_tag="ACHOQW_005194"
     CDS             648..>726
                     /locus_tag="ACHOQW_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HFP9576049.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNI"
BASE COUNT          232 a          108 c          152 g          234 t
ORIGIN      
        1 tatctgatat ttaccttgaa aatatccata aacaagaatc ataccctgcc agtgagaggg
       61 ataattatct gttaggctta ttaagagaag agttaaaaaa tatcccagaa ggtaaggact
      121 ctttgattga gtcatatgca gaaaaaagag aacatacttg gtttgatttt ttcaggaatt
      181 tggccatgtt gaaggctgga agtttgttta cagagacggg aaaaactgga tgccataaca
      241 tatcgccctg tagcggatgt atatatcttg atgccgacat gattattacc gataaattag
      301 gagtcctgta tgctcctgat ggtatcgctg tgcatgtaga ttgtaatgat gagataaaaa
      361 gtcttgaaaa tggtgcgata gttgtcaatc gtagtaatca tccagcatta cttgcaggcc
      421 tcgatattat gaagagtaaa gttgacgctc atccatatta tgatggtcta ggaaagggta
      481 tcaagcggca ttttaactat tcatcgttac acgattataa tgctttttgt gattttattg
      541 aatttaagca tgaaaatatt ataccgaata ccagtatgta tacctgcagt tcatggtaag
      601 tcttccggtt gtggttgaaa aatatcgttc tttaggtata gattcagatg attaatcctg
      661 ttactaatac tcagggcgtg tcccctataa atactaaata tgctgaacat gtggtgaaaa
      721 atattt
//
LOCUS       DAZKFZ010000535          724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000535 DAZKFZ010000000
VERSION     DAZKFZ010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.539"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            145..>724
                     /locus_tag="ACHOQW_005195"
     CDS             145..>724
                     /locus_tag="ACHOQW_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576050.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPV"
BASE COUNT          229 a          148 c          140 g          207 t
ORIGIN      
        1 ggtatgactt accgggagtg attgtttctg agtgaacaga ggccactttc gcctgataaa
       61 tgaaaaatga gcgatttttg caagttagtt tttctatagc cgagtacgct cagcgcctcg
      121 cttgattaca atgaggttgc tgctatgagt cctgataata tcggttcggc tgtcaatcgc
      181 ttctcaggtt ggccatctaa tgattctcta aaaacagata aagaatcaat gacatcaccc
      241 gtgactgtgc aggtaataaa gatgattgcc gccatccttg acccacatcg gatagtcgtt
      301 ggaattgata cactttatac aacgcagcgc gaaacattta cctcagagag tcaacagctc
      361 attgaatcag cattgcaaac acttcatgtg atgaatttaa ataatctgga aacaaatcgc
      421 gatgaagaaa gtcactatct ggcttttgaa tataacgttg acacctctga cagaataacc
      481 gtttctgctt ataatgaaaa aaaacctggc tgctatggcc aatgtcttgt aatacaggat
      541 gacgaaacag acttatttaa cctgataaaa attaaaattt tcgaagccag gcataattat
      601 gtcgctcaat tgatgagcga tccggaattt atgttcaaat tcagttatag cgctcaacaa
      661 aatagattag aacctcatct tctggttccc gcttatttcc ctctaaaaac aaacgaaccg
      721 gtta
//
LOCUS       DAZKFZ010000536          723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000536 DAZKFZ010000000
VERSION     DAZKFZ010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.540"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            280..587
                     /locus_tag="ACHOQW_005196"
                     /pseudo
     CDS             280..587
                     /locus_tag="ACHOQW_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          232 a          163 c          143 g          185 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcagga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttaagctc tgtgcgcttt taacaaaacg tccctttctc
      721 cac
//
LOCUS       DAZKFZ010000537          721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000537 DAZKFZ010000000
VERSION     DAZKFZ010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.541"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..98
                     /locus_tag="ACHOQW_005197"
     CDS             <1..98
                     /locus_tag="ACHOQW_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576051.1"
                     /translation="IVTNPADYDKYSDMVKKHVIDGEELIIKYFM"
     gene            complement(236..565)
                     /locus_tag="ACHOQW_005198"
                     /pseudo
     CDS             complement(236..565)
                     /locus_tag="ACHOQW_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          203 a          155 c          164 g          199 t
ORIGIN      
        1 atattgttac gaatccagcc gattatgata aatattccga tatggttaaa aagcatgtaa
       61 tagatggtga ggaactcata attaaatatt ttatgtaatt aattaggcgt aattgtgtca
      121 ctggaataga ggtttcttgt gacgcatgtg gaaggagcgt ctatggacaa ggcgtttcct
      181 gctggtgata aataaaggcg cagcacgtcg aatgcaagaa tgcgctgcgt ctggctcatg
      241 gcaattcatt atcttcgcat agcgtcaatg acaaaaatcg actgtgtgga tcaaattgat
      301 aatcagcaaa cggttcgcaa tcaacgaaac catgcttcaa ataaagttga tggcaggccg
      361 taaatccagc ctgtgtaccc gtttctaagc tcaggcgatg aaggcatctg tcatgggcga
      421 cctgcaaaat gtggcgtaaa atcagactgg cgacaccccg acgtaaataa tttggcgcgg
      481 tacgcattga tttcagttcg ccatgtttat catccagcaa ctttagcgca cccattcctg
      541 cgagttgttc gccttcccat actgacctgt tgacctggct tgtttaatga catccgatct
      601 caccgcgtgg ggttatggat ggggcaaact cactcaattt ctggtttaaa atggaaacct
      661 gattctgaaa gttttctgcc acccacgatc cgtatacgcg ataaaccctc tgatcatcag
      721 a
//
LOCUS       DAZKFZ010000538          714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000538 DAZKFZ010000000
VERSION     DAZKFZ010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.542"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            504..644
                     /locus_tag="ACHOQW_005199"
                     /pseudo
     CDS             504..644
                     /locus_tag="ACHOQW_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          213 a          137 c          143 g          221 t
ORIGIN      
        1 cggcgaatac tgatagaaac attgaaatat tgtaagagaa agcgttcaaa attctgtggc
       61 acgtgaaaaa ttctacagcg tcatcacctt attcagtcga ccttcaaaat aaacgtttaa
      121 cagagacaat caccatttgg attgtccgct gaggaggcga acaatttcct agacagcttt
      181 atcatgtcag cccacattgt tggcgctggc aatactaagg acaaacgatg gggaagtagc
      241 tttttagcgt tattttcggg agtaaaaaac gtggtatctt tccgtatgtc agttgtatct
      301 tgctggtatt cgtagtcgtg ataaggcata caacgatatt tatcttgacg ttagtgaaga
      361 ataagttgtt ttgtgatcta tgttaatctg ggattacaat ccgggcttga tacatcgcat
      421 cacgactttt attctcttca gaatagaata tgttactccc cgctttacag gctcaggatt
      481 tgaattacaa attatacttt taagtgaaca ataaccaagg gtatttctta tttcctgaaa
      541 ccaggcttgg cccatttcgg gatgagtttg aaaaaactct tgacgcatac gatagtgatg
      601 aaataaacga aacaaattac gtcaacaaac tcaggcgtct tgcctgacat gctcctgaca
      661 tcattgatat tcacgcttat ctggcatatg catttgcgct ctttatgctg caat
//
LOCUS       DAZKFZ010000539          713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000539 DAZKFZ010000000
VERSION     DAZKFZ010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.543"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..201
                     /locus_tag="ACHOQW_005200"
     CDS             <1..201
                     /locus_tag="ACHOQW_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9576052.1"
                     /translation="DDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEK
                     HGSQVDLIFRFLDHAFATGVLG"
     gene            214..588
                     /locus_tag="ACHOQW_005201"
     CDS             214..588
                     /locus_tag="ACHOQW_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9576053.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            585..>713
                     /locus_tag="ACHOQW_005202"
     CDS             585..>713
                     /locus_tag="ACHOQW_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9576054.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQN"
BASE COUNT          183 a          168 c          195 g          167 t
ORIGIN      
        1 gacgatccgc atcacctgat tggtcatggt cagggcggaa tggggacaaa atctcacgat
       61 attttcacgc taccgctgtg tcgggagcat cacaacgagc ttcatgcgga tccgctggcg
      121 ttcgaagaaa agcatggttc tcaggttgat ttaatttttc gttttcttga tcacgccttt
      181 gcaactggcg tgctcgggta aaagaggtga ctgatgctca tagatttggt tttaccttac
      241 ccgccgacgg tgaacactta ctggcgacgc cgtggcagca catattttgt atcaaaagcc
      301 gggaagcgtt atcgccgggc agtggcgctt attgttcgcc agcagcgact gaaattaagc
      361 ctgtccggac ggctggcaat aaaaattatt gcagagccac cggataagcg ccgccgcgac
      421 ctggacaata tcctgaaagc accgctggat gcgctgacgc acgcggggtt gttaatggac
      481 gatgagcagt ttgatgaaat caatattgta cgtggaaagc cagtatctgg tggacgtctg
      541 ggggtgaaga tttaccccat aatgcttgaa gggcaggtca aaaaatgaaa ctggaagatt
      601 taccgaaata ctactcccca aaatcccctg gcctgaccga tgcatcggcc tcaacgtcaa
      661 aagatgcgct gagtatcact gatgtgatgg ccgcgcaggg catgacacag aat
//
LOCUS       DAZKFZ010000540          712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000540 DAZKFZ010000000
VERSION     DAZKFZ010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.544"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..463)
                     /locus_tag="ACHOQW_005203"
     CDS             complement(<1..463)
                     /locus_tag="ACHOQW_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9576055.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGE"
     gene            complement(496..573)
                     /locus_tag="ACHOQW_005204"
                     /pseudo
     CDS             complement(496..573)
                     /locus_tag="ACHOQW_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(575..>712)
                     /locus_tag="ACHOQW_005205"
     CDS             complement(575..>712)
                     /locus_tag="ACHOQW_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9576056.1"
                     /translation="IIPEQVRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGM
                     K"
BASE COUNT          203 a          210 c          112 g          187 t
ORIGIN      
        1 cttcaccagt caaacgagaa aatgcagcgg catcaaataa agtacataca tctgaaccat
       61 ctgaattctt cacaaaaaca cctttttcag gctgctccag tgtaattgga cattgaagcg
      121 catcaccagg acattgaagt ttttcaatat cgacagtaaa ggcacactcc gatatttttt
      181 cctgcaaaga gtttcgccct cccattccag ggagcgcact taatatcctg tttctgagca
      241 cattaactcc atcattaaaa gcaatattca ataattctgc ataaacccgt ccctgccctt
      301 cactttctgc taaaaaacgg cctgtatctg gcatataaga aactctaacc ctctcccctc
      361 ccaggagaaa tccaccaatc tgcccctcca ttacctgctg acgcagctcc atcatctcat
      421 tcacgaatga agccgatgac acatatgata ttaatccagg cattaccatg ctccttcata
      481 cggataaata ttccctcaag tcttcattaa caaacatcca ccggacatga caacaaaaac
      541 cggagccgga ctccggtttt gtgaagctgt cgggttactt catcccgcca atattttccc
      601 acgtcccgtc agcacgcagg atttgcagcg gtcttaccac acactgtatc tgctttttat
      661 ccgcatccag tatcaccacc tgcgtgatta ccctgacctg ctccgggata at
//
LOCUS       DAZKFZ010000541          702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000541 DAZKFZ010000000
VERSION     DAZKFZ010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.545"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..310
                     /gene="ybcN"
                     /locus_tag="ACHOQW_005206"
     CDS             <1..310
                     /gene="ybcN"
                     /locus_tag="ACHOQW_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP9576057.1"
                     /translation="LSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVD
                     VVTGEITTIQSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA
                     "
     gene            310..480
                     /locus_tag="ACHOQW_005207"
     CDS             310..480
                     /locus_tag="ACHOQW_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9576058.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            473..>702
                     /locus_tag="ACHOQW_005208"
     CDS             473..>702
                     /locus_tag="ACHOQW_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9576059.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECA"
BASE COUNT          194 a          179 c          174 g          155 t
ORIGIN      
        1 actcagccac atgtggtaca gcgaaatcag tgaatacctc atcagcaggg ggaaatcgtt
       61 cgctacttca gcatgggtaa aagatgctct caaacacaca tacctcggtt atgaaaccaa
      121 ggacctggtt gatgtcgtaa ccggcgaaat cactactatc cagtcgttac gccatacctc
      181 cgatcttggt accggagaga tgtatgtctt cctgtgtaag gttgaagcct gggcgatgaa
      241 tattggctgc cacctgacta ttccacagag ctgcgagttc cagctgctgc gtgacaagca
      301 ggaggcgtaa tggctacacc gcttattcgt gtcatgaacg gacacatcta caaagtacca
      361 aatcgtcgta agcgtaaacc tgagctgaag ccatccgaaa taccaacact gctcggatat
      421 accgccagcc tggttgataa aaaatggttg cgactggcag caaggaggaa tcatggctga
      481 tttgagaaaa gcagcgcgta gtcgggaatg ccaagtaaga atccctggcg tatgtaatgg
      541 caaccctgaa acgtctgtac tggcacatat ccggctgact ggattgtgcg gcaccggtac
      601 caaaccgcca gacttgattg ccaccattgc atgttctgcc tgccacgacg aaatcgaccg
      661 ccgcacacat tttgtcgatg ctgcatatgc aaaagaatgc gc
//
LOCUS       DAZKFZ010000542          701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000542 DAZKFZ010000000
VERSION     DAZKFZ010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.546"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            25..657
                     /locus_tag="ACHOQW_005209"
     CDS             25..657
                     /locus_tag="ACHOQW_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9576060.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          242 a          106 c          149 g          204 t
ORIGIN      
        1 agcatttaca ctcaggatga atatatggga aaaattaaaa ttgtagtttc agatcagcag
       61 ccgtttatga ttgatgggat aattggattc ctcggacatt atcccgattt atatgaggtt
      121 gttgggggct ataaagatct gaagaaagct atagcggagt gtaacaaatc tgcagcccaa
      181 atatttattt taggagaatt tgcaggtgga atgatggggg ccgaactcgt aaaatgggtt
      241 aaatcgcata aaatagatgc tcatattatt acatttgtag caaaaatgcc gtatattgat
      301 tcaataaaat tgcttgaagc aggtgcgaaa ggatgcgtat ggaaaaccag tcacccggcg
      361 aaacttaatc gcgctattga ttcgattagt aacggctaca cttattttga tagtgtacat
      421 atggattgtg agaaaatatc ttccagatat tcctctgata atcaacttac aaatcgtgag
      481 tctgaaatat tacaactcat agctgatgga aaaacaaaca aagaaatcgc taatttcttg
      541 cagttaagca ggaaaacagt cgaaactcat agactcaata tcatgaagaa attagatgtt
      601 cacagtggta tcgagttgat caaaacagct ttacgtatgg gtgtgtgcac tatataaaaa
      661 aaagaggctt taggctcatt ttgctaaagc cttacgattg t
//
LOCUS       DAZKFZ010000543          695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000543 DAZKFZ010000000
VERSION     DAZKFZ010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.547"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>695
                     /locus_tag="ACHOQW_005210"
     CDS             <1..>695
                     /locus_tag="ACHOQW_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9576061.1"
                     /translation="YKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPL
                     SLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAV
                     LAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIRQAAENL
                     ARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYN
                     FLSVSQLRHIDEL"
BASE COUNT          148 a          176 c          233 g          138 t
ORIGIN      
        1 gtataaggct gccatgcgca cactgcccgc acagttcacg gatattgtca ctcagcttgc
       61 cggtggtcag aatcccttcc ttatcatgct gcagcagggg gggcagatca gcgattcatt
      121 cggtggaccg ctcagcctgc ttaccctgct gaaggaggaa cttctcggga tcagggatgc
      181 ctctgaatca tcagaggagt cgctgtcaga tacggcaaat gcactggctg aaaatgcccg
      241 gaatgccggt gagctgggac gatttatgtc ggtggcccgt gtggcggcag gtggcggggt
      301 tgccgtactg gccgcgcttg ctgccgccgc ctggcaggca gagcaggctg accgggcctt
      361 attgcgttca ctgatcctga ccggaggggc ggctgccacc acaacggcag aattgtggaa
      421 aatggccggg gtgatcagcg atgaagccgg tggtggtatc agacaggcgg cagaaaatct
      481 ggcccgtctg gcagaaagcg ggaaatatac cgccgggcag ctacggatca tgggggaaac
      541 ctctcagaga tggctgcaga cggtggggga cgatgccggg aaggtggaaa aagcctttga
      601 agggattgca gcagatccgg tgaaggcgct ggcctccctg aatcagcagt ataacttcct
      661 gagcgtttcc cagttacgcc atattgatga gcttg
//
LOCUS       DAZKFZ010000544          694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000544 DAZKFZ010000000
VERSION     DAZKFZ010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.548"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>694
                     /locus_tag="ACHOQW_005211"
     CDS             <1..>694
                     /locus_tag="ACHOQW_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9576062.1"
                     /translation="DWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVA
                     FIIDVFAGYIVGWQVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQR
                     LKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHIPPA"
BASE COUNT          171 a          159 c          213 g          151 t
ORIGIN      
        1 gactggctga agaaagagat acagcgcgta tacgatgaaa atcaccaggt atacggtgtg
       61 cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag tggccagatg cactgtggca
      121 cgtctcatgg cagttatggg acttgccggt gttctccggg gtaaaaaggt ccgcactacc
      181 gtcagccgga aagccgttgc cgcatgcgac cgcgtaaacc gtcagttcgt ggcagaacgt
      241 ccagaccagt tgtgggtggc tgattttacc tgggtaagca catggcaggg cttcgtttat
      301 gtggcgttca tcattgatgt gttcgccgga tacatcgtgg gatggcaggt ctcatcatcc
      361 atggaaacaa cattcgtgct ggatgcactg gagcaggcgt tgtgggcccg tcggccgtcc
      421 ggcacaatcc atcacagtga taaaggttct cagtatgtat cgctggccta cacgcagcgg
      481 cttaaggaag ccggattact ggcatcaaca gggagtactg gcgactcgta tgacaacgcg
      541 atggctgaga gcatcaatgg tctttacaaa gcggaggtaa tacaccgtaa gagctggaaa
      601 aaccggacag aagtggaact ggccacacta acgtgggtgg actggtataa caatcgacga
      661 ttgctggaaa ggctgggcca tattcctccg gcag
//
LOCUS       DAZKFZ010000545          689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000545 DAZKFZ010000000
VERSION     DAZKFZ010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.549"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..640)
                     /locus_tag="ACHOQW_005212"
     CDS             complement(<1..640)
                     /locus_tag="ACHOQW_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9576063.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQ"
BASE COUNT          197 a          135 c          141 g          216 t
ORIGIN      
        1 tttgttgaag tgggtatatg ggtaatgcgt aaaaacattg ttttgttata ttaaaatgac
       61 acttgtcttt gcttataatc atttctggag caaaggggtc acggctgagg gggtggagag
      121 cgttacgcag gataagttca gtcaatgctt cctgatcata taatgcacat atttctgaat
      181 ttcgagcatt tctgacaaaa acacctgtct caggaatatg tagcgttatc ggacaattaa
      241 gatgttcctc agtacaggaa aagtcttgta aactaacggg aaatgcattc gaatttattc
      301 gatctagtag tgtttttatg acgctctcct gcacaagcgg ggcagcttgc ctctgctcca
      361 gatataagct gtatgcctgc agaaaagtgt tacctccgtt aagttgtctt gtcaggcggt
      421 ctgcgagatg atgagcctct cttgccccaa gatggtccca aagtccacca agaataggct
      481 caacactaaa accatccata aactggatat aatgaagcct catattacgg cctcctatag
      541 taatagtgtc attctggttg cttcttgcta tatgtcgtaa agcctccagc tcattttccg
      601 gcaatacact tccattagaa aaatttaata tgactggcat attattatcc tttttaatcc
      661 atattgtgaa ctatattggt aacaagaat
//
LOCUS       DAZKFZ010000546          688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000546 DAZKFZ010000000
VERSION     DAZKFZ010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.550"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            429..>688
                     /locus_tag="ACHOQW_005213"
     CDS             429..>688
                     /locus_tag="ACHOQW_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576064.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQL"
BASE COUNT          177 a          124 c          184 g          203 t
ORIGIN      
        1 ttgttgttct gcgggttctg ttcttcgttg acatgaggtt gccccgtatt cagtgtcgct
       61 gatttgtatt gtctgaagtt gtttttacgt taagttgatg cagatcaatt aatacgatac
      121 ctgcgtcata attaattatt tgacgtggtt tgatggcgta gatgcacgtt gtgacatgta
      181 gatgataatt attatcattt tgcgggtcct ttccggcgat ccgacaggtt acggggcggc
      241 gacctcgcgg gttttcgcta tttatgaaaa ttttccggga tccatgtccg gtttctcttc
      301 aagttaacta tatgaaaaat ataaaaacag gtcttctgtg aaccggacat gaacaaaaaa
      361 cagacatgta aaccggacat gaccggtttt gttgtgattg tgaggtgaga gtttttgcga
      421 ggtgaggagt ggctacgcag actgaagttg ccaggcattt aagtctgacc gatcgccagc
      481 ttcgcagatt gcagaaattg ccgggtgccc cgatatcgaa taagcgaggg caactggatc
      541 tggatgcctg gcgcgatttt tacatatcgt atctgaggag aagtaaaaac gatgtgcctg
      601 atggcgatag cgaagacgac tatgaggaga aattgcttat tgccagatgg gaactgacag
      661 cagaacaggc tgttacacag cagttaaa
//
LOCUS       DAZKFZ010000547          679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000547 DAZKFZ010000000
VERSION     DAZKFZ010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.551"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..262)
                     /locus_tag="ACHOQW_005214"
     CDS             complement(<1..262)
                     /locus_tag="ACHOQW_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576065.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFGEHCHHIYLDD"
     gene            complement(249..587)
                     /locus_tag="ACHOQW_005215"
     CDS             complement(249..587)
                     /locus_tag="ACHOQW_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576066.1"
                     /translation="MKNVENIIWHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRE
                     RKRFEFTGVEHLSQVMDIAELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFK
                     TMRVIDNECA"
     gene            complement(592..>679)
                     /locus_tag="ACHOQW_005216"
     CDS             complement(592..>679)
                     /locus_tag="ACHOQW_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576067.1"
                     /translation="FELDCLLTRVSASSNQFRNSNGYLPENS"
BASE COUNT          209 a          152 c           96 g          222 t
ORIGIN      
        1 tatcatcaag gtagatgtga tgacaatgtt caccaaacat actcatcact tccagaccgt
       61 ttacagatgt ccatctttgt tcactttccc aataaacaag aaccggtttg cgacaggtat
      121 atctgcctaa aaagcgccga atgtgttctt cctgattctc gtaaaatatt gcctctgcat
      181 tcgagatttg ccgacgcatt cgccagcgga ttttttcttc ataaaaagac aagttgtcga
      241 atttgacatt acgcacattc attgtcaata actcgcatgg ttttaaatgt aaaacttaaa
      301 aaaccaccaa acaaatgaag tgaaatttta tacttctttg ccttataaac aattgtcgca
      361 tctttaatat tacctgcatt ttggttatct ataagttcag caatatccat aacctgcgat
      421 aaatgttcca ctcctgtaaa ctcgaacctt tttcgttccc ttccggctgt aaattcatca
      481 acataaagct ccgccgtcaa tataaaatta tactcggtgt ggtgacattc cattctggtt
      541 tctactaaaa caccatcatg ccagataata ttttctacat ttttcatgct attaactatt
      601 ctcaggtaag taaccattag agtttctaaa ctggttcgat gacgcagata ctctcgttaa
      661 caaacaatct agctcaaat
//
LOCUS       DAZKFZ010000548          678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000548 DAZKFZ010000000
VERSION     DAZKFZ010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.552"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>678
                     /locus_tag="ACHOQW_005217"
     CDS             <1..>678
                     /locus_tag="ACHOQW_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9576068.1"
                     /translation="LYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQR
                     LRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFIIHGP
                     QLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVISRSV
                     ICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDERFMN
                     RKISFRGS"
BASE COUNT          211 a          151 c          125 g          191 t
ORIGIN      
        1 ctctacgact tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag
       61 catttaagca ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag
      121 tcgttacaac gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt
      181 atcgcgccga cgacaacagg gttaaatctg catcaccatc ttgagaaaaa tctcagaggc
      241 cttgaacaaa caattaatat agtcaataag tccgaactaa agaaaaactt tattattcac
      301 ggccctcagc tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat
      361 tcctctgtcg agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg
      421 ttggttcacc gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc
      481 atttgcatgc cattgcatac gattcgcaat actctaattt gtagtaatag gcatccacga
      541 attactgaca atagcactta tgaacaaatt atggcagaag agttcaccca attaatatct
      601 aaatcagcag gggttgatga tattcaaatg gaaatagacg agagatttat gaatcgcaaa
      661 atttcatttc gtggttcc
//
LOCUS       DAZKFZ010000549          678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000549 DAZKFZ010000000
VERSION     DAZKFZ010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.553"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>678
                     /locus_tag="ACHOQW_005218"
     CDS             <1..>678
                     /locus_tag="ACHOQW_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576069.1"
                     /translation="LTFKEGINSLSEALEHMNIDAIMSVIGLVQYARMIKMNDNISAI
                     DHAGAVSDIKNIVDKFLGGILTLTNNRVYNPGGVSGASLEGFISSGLEVCASRMGGTA
                     GRYLSNVAKVIKLPLLDIGINIWSLYDSSLNHAKATTQIEYISTAIDVSFSSINTALS
                     IGAIAYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSIN
                     KATGIIDL"
BASE COUNT          225 a          107 c          133 g          213 t
ORIGIN      
        1 ttaactttta aagaggggat aaatagttta tccgaagcac tcgaacacat gaatatcgat
       61 gcgattatgt ctgttattgg cttggttcaa tatgcacgaa tgataaaaat gaatgacaat
      121 attagtgcaa tagatcatgc aggagcagta tcagatataa aaaatattgt agacaaattt
      181 ttaggcggaa ttttgactct tactaataat cgcgtatata atcctggcgg tgtatctggt
      241 gcctcgcttg aaggatttat atcttctggg ttggaggtgt gtgcttcaag aatgggtggc
      301 accgcagggc gttatcttag caatgtggca aaagtaatta aattaccttt gctagatata
      361 ggcataaata tttggtcatt atatgattct tcattaaatc atgctaaagc caccacacaa
      421 attgaatata tatcaacagc aatagatgta tctttttcat ctataaatac agctttgtca
      481 ataggcgcaa tcgcatatcc tcctttggca atagctatag ttccgataac tattttttct
      541 catgaagtaa aaaactatgc tgtttatgtg aatcaaatta atgagcgcca taagttatgg
      601 ctcgaagctg aaaagtatct ggacaatgga agtgcaaagg ttctaagcat taacaaagcc
      661 acaggcataa tcgattta
//
LOCUS       DAZKFZ010000550          675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000550 DAZKFZ010000000
VERSION     DAZKFZ010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.554"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>675
                     /locus_tag="ACHOQW_005219"
     CDS             <1..>675
                     /locus_tag="ACHOQW_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9576070.1"
                     /translation="SLKVGKIGAVNKNSTENGYVSAQKSPKNGTLCCMENNQRHPNFP
                     SKTPKNGSRNPKEPKDLNPTHNARESAPTSEQEVLSLQAAPHVFLDGLSEPIGKFPMT
                     DSWYPSRDFRRRAALWGMALPETEFTPAELTAFRDYWAAEGKVFTQIQWEQKFARHVN
                     HVRAQVKPVSKGVNHAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGG
                     GLFEPL"
BASE COUNT          199 a          160 c          187 g          129 t
ORIGIN      
        1 gtctttaaag gtgggtaaaa ttggtgctgt taataaaaac agtaccgaaa acggttatgt
       61 ttcagcacaa aagagcccca aaaacggaac tctttgttgc atggaaaata accaaagaca
      121 cccaaatttt ccatcaaaga cacccaaaaa cggatcacgg aacccaaagg aacccaaaga
      181 tctaaacccc acacataacg cacgcgagag tgctccgacc agtgagcagg aagttttgtc
      241 gttacaggca gccccccatg tattcctgga tggcctgagc gaacccatcg gaaaatttcc
      301 gatgaccgat agctggtatc cgtcacggga ttttcgacga cgggctgcgt tgtgggggat
      361 ggctttgccg gagacagaat ttacacctgc tgaacttacc gcattccggg actactgggc
      421 agcggagggg aaagtgttta cgcagattca gtgggagcag aaattcgccc gtcacgtaaa
      481 tcacgtcagg gcgcaggtta aaccagtcag caagggggta aaccatgcag cagcaccagg
      541 tggcaccgca tcacgggcag ttcaggaaat tcgggcagca cgtgagcagt gggaacgtga
      601 aaacggattt atcagcgacg gaaacggcct ggaagctgtg ggaactcatg ggggaggttt
      661 attcgaaccg ctgga
//
LOCUS       DAZKFZ010000551          674 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000551 DAZKFZ010000000
VERSION     DAZKFZ010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.555"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..590)
                     /locus_tag="ACHOQW_005220"
     CDS             complement(<1..590)
                     /locus_tag="ACHOQW_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFP9576071.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSV"
BASE COUNT          191 a          163 c          148 g          172 t
ORIGIN      
        1 ctaactgagt gataatattc aagataacca ccggttaaat ttaacagccg acgcgccatc
       61 gcatcgccag aaaatgttcc accagaaaca gccgtcccga catggacata acgtgaagct
      121 gggccatatt gttgcgtgat ccttttcagg ttatcggcaa ttaaggtcgt cgcctcatcc
      181 catgagatac gctcaaattt accctcgcct cgtttaccta cccgcttcat cggatatttc
      241 aaacggtctg gatgataaac aaatttacga tagccgcgac cacgaacaca ggcgcgcata
      301 atcggcattt gtggatccaa ttcgttatcg ggaagcgtgg taatttgcgt caccacgccg
      361 tcgcgcatat gcgcccgaat atcgcactta ccaccgcagt caaatgtgct acaggtgggc
      421 acgactttat cagctactgg agctgtaact tctggcgacg catctgggga ttgcgccttt
      481 gcggtagaag aaataaaagg caaagtgata agcgcggaag tcgcttgtaa aaaactacgt
      541 cgtgatactt gcggaaatgt aatgcgttcc ttttcgtctg gcgtatccat cttgcgttcc
      601 cgaaaatgaa aaaataaata ttcacgtatt tattcgagaa caatgctgat aacaatcaaa
      661 taattagtcg ttta
//
LOCUS       DAZKFZ010000552          672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000552 DAZKFZ010000000
VERSION     DAZKFZ010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.556"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..672
                     /locus_tag="ACHOQW_005221"
                     /pseudo
     CDS             1..672
                     /locus_tag="ACHOQW_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
BASE COUNT          176 a          137 c          177 g          182 t
ORIGIN      
        1 agttctggca atgctggtcc cggcgttatt agttgctggc gcagcaaatg cggctgaaat
       61 ttataataaa gatggcaata aactggattt gtacggaaaa gtagcgggcc tgcactactt
      121 ctctgatgat gctagcagcg atggcgacat gtcatatgcc cgtatcggtt tcaaaggtga
      181 aactcagatc gctgaccaat tcactggtta tggtcagtgg gaatttaaca ttggcgcaaa
      241 cggtcctgaa agcgacaagg gtaataccgc aacgcgtctg gcatttgcag gtttcggctt
      301 tggtcagaat ggtactttcg actatggtcg taactacggt gtcgtatatg acgtagaagc
      361 atggaccgat atgctgccag aatttggtgg agatacctat gctggcgctg acaacttcat
      421 gaacggtcgt gctaacagcg tagcaaccta tcgtaacaat ggtttctttg gtcaagttga
      481 tggtctgaac tttgcactcc agtatcaggg taacaacgag aaaagcggat tatttgatca
      541 agaaggttca ggtaacggta atggacgtaa acttgctaaa gagaacggcg acggttcagt
      601 atgtccactt cctatgactt tgactttggt ttaagtctgg gcgctgctta ctctaactct
      661 gaccgtaccg ac
//
LOCUS       DAZKFZ010000553          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000553 DAZKFZ010000000
VERSION     DAZKFZ010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.557"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            10..570
                     /gene="pagP"
                     /locus_tag="ACHOQW_005222"
     CDS             10..570
                     /gene="pagP"
                     /locus_tag="ACHOQW_005222"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP9576072.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          187 a          135 c          155 g          193 t
ORIGIN      
        1 tggtcacaaa tgaacgtgag taaatatgtc gctatctttt cctttgtttt tattcagtta
       61 atcagcgttg gtaaagtttt tgctaacgca gatgagtgga tgacaacgtt tagagaaaat
      121 attgtacaaa cctggcaaca gcctgaacat tatgatttat atattcctgc catcacctgg
      181 catgcacgtt tcgcttacga caaagaaaaa accgatcgct ataacgagcg accgtggggt
      241 ggcggttttg gcctgtcgcg ttgggatgaa aaaggaaact ggcatggcct gtatgccatg
      301 gcatttaagg actcgtggaa caaatgggaa ccgattgccg gatacggatg ggaaagtacc
      361 tggcgaccgc tggcggatga aaattttcat ttaggtctgg gattcaccgc tggcgtaacg
      421 gcacgcgata actggaatta catccctctc ccggttctac tgccattggc ctccgtgggt
      481 tatggcccag ttacttttca gatgacctac attccgggta cctacaacaa tggcaatgtg
      541 tactttgcct ggatgcgctt tcagttttga gacaaatgaa gttttagtaa cttctttaaa
      601 atcaataact aaaataagta acatcaaaaa taacgcgatt tttatcactt tttagtaaag
      661 ttgcactgga
//
LOCUS       DAZKFZ010000554          660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000554 DAZKFZ010000000
VERSION     DAZKFZ010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.558"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            109..>660
                     /gene="nleB2"
                     /locus_tag="ACHOQW_005223"
     CDS             109..>660
                     /gene="nleB2"
                     /locus_tag="ACHOQW_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFP9576073.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISKAQDSLISMYAKKRNHAWFDF"
BASE COUNT          236 a           85 c          144 g          195 t
ORIGIN      
        1 gaaagcaaag atggcaaaac tgctggaggt tttgtggttg agtatgccaa tataattaat
       61 agattaaaga gttagttgtg aagaaaatat ggataaacag gacgacgaat gctttcaccg
      121 ataaggacaa ctttccataa ctcagtaaat atagtgcaga gttcaccctg tcaaacggtt
      181 tcttttgcag gaaaggaata tgagttaaag gtcattgatg aaaaaacgcc tattcttttt
      241 cagtggtttg aacctaatcc tgaacgatat aagaaagatg aggttccaat agttaatact
      301 aagcagcatc cctatttaga taatgtcaca aatgcggcaa ggatagagag tgatcgtatg
      361 ataggtattt ttgttgatgg cgatttttca gtcaaccaaa agactgcttt ttcaaaattg
      421 gaacgagatt ttgaaaatgt aatgataatc tatcgggaag atgttgactt cagtatgtat
      481 gacagaaaac tatcagatat ttatcatgat attatatgtg aacaaaggtt acgaactgaa
      541 gacaaaagag atgaatactt gttgaatctg ttagagaaag agctgaggga aatttcaaag
      601 gcgcaggatt ctttgatttc tatgtatgca aagaaaagaa atcatgcatg gtttgatttc
//
LOCUS       DAZKFZ010000555          651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000555 DAZKFZ010000000
VERSION     DAZKFZ010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.559"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            184..408
                     /locus_tag="ACHOQW_005224"
                     /pseudo
     CDS             184..408
                     /locus_tag="ACHOQW_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(401..>651)
                     /locus_tag="ACHOQW_005225"
     CDS             complement(401..>651)
                     /locus_tag="ACHOQW_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9576074.1"
                     /translation="QFFRLLNRRYEKASIILTSNKGFADWGEMFGEHVLATAILDRLL
                     HHSTTLNIKGESYRFNIPDRLVVHLSLHARPELRAHNT"
BASE COUNT          168 a          159 c          156 g          168 t
ORIGIN      
        1 acttcctaga aaacaataca tttttcagta ttacaggtat agatgagtct ctctatgaaa
       61 agcatataat ctttatcact cttaaggtta ttgtcccctc aaaaaagtag cgctgatttc
      121 ctgtatgcgg aatttgctga ctaatcttac ccagcatcag cagacgttcc aaaggccacg
      181 acatacttcg cgaaacgcct gatggatgaa ctgcgtgccc agggttacca ttttaaagta
      241 aaaaccgtga cggaaagcct gcgccgtcac ggactgaggg caaaagcttc ctggaatttc
      301 agtccggtct gttaccgcgc acacagccag cctgtgtcag aaaatctgct ggagcaggat
      361 ttttacgcca gtggcccgaa ccagaagtgg gcgggagaca tcacgtattg tgtgcccgga
      421 gttcagggcg agcatggagg ctcaaatgaa ccacgagtct gtctggaata ttgaaccggt
      481 aactctctcc tttgatattc agcgtggttg agtgatgtag cagccggtcc aggatcgctg
      541 ttgccagcac atgctctccg aacatttctc cccagtctgc gaaccctttg tttgacgtca
      601 gtatgatgct cgctttttca tatcgacggt tcagtagccg gaagaactga a
//
LOCUS       DAZKFZ010000556          646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000556 DAZKFZ010000000
VERSION     DAZKFZ010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.560"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            6..184
                     /locus_tag="ACHOQW_005226"
                     /pseudo
     CDS             6..184
                     /locus_tag="ACHOQW_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            513..>646
                     /locus_tag="ACHOQW_005227"
     CDS             513..>646
                     /locus_tag="ACHOQW_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
                     /protein_id="HFP9576075.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     "
BASE COUNT          190 a          117 c          171 g          168 t
ORIGIN      
        1 gtgatatggc gttaacacac cgcgaactct gtcagattgc gtacaagttc cttaagcgca
       61 acggtttcaa ggtttgtttt catgaccgct ttatagctgt aaccagtacc ggagaacagc
      121 cagatgctat gggattcaga aattcagcat catgcctgat agaggcgaaa tgttctcgtg
      181 ctgacttgtt ggcagataga aaaaagcgtt ttcgtaaaaa tccgtctctt ggaatgggcg
      241 actggcgatt ctttattagt gagccgggaa ttatttcaat tgaggattta ccacctggct
      301 ggggattact tcacgttgtt aacggaagag tacggaaagt acatgggtgg cccaagggta
      361 attgctgttg gggtaatcct gacgataagc catttactgg aaataagtga atgcgattac
      421 atgttgtctg cattaaggcg catggagttg agagggcacc ttaatgaaat atatgacggt
      481 gtaattgtta ataagaaaga aggaaacgcg gcatgaccac tattaccgac aaagaactga
      541 ttaaagaaat caaagagcgc ataggcagct tggacgttcg agacaatatt gagcgccgtg
      601 cttatgaaat tgcactggca tcgctggaag cagaaccgat agcgtg
//
LOCUS       DAZKFZ010000557          644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000557 DAZKFZ010000000
VERSION     DAZKFZ010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.561"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>644
                     /locus_tag="ACHOQW_005228"
     CDS             <1..>644
                     /locus_tag="ACHOQW_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFP9576076.1"
                     /translation="YEMENDAPPKILIPIDHNLNFIDAKYNGETYRGNEEFAINLFLQ
                     HILHNDIQEQTSIDLYTSIINKELDSNRKSYNNEIFNNFSFDKSVKLNSYNYIADDIE
                     QVIDKGSKVQLEVYNLLSEEKIFEHKIMNNWTRSIKNILTTYLFMSSGAVTARNVQTF
                     SPTINNESRIRLPRALPVGHPYPEEHKASGFSPFMMGGLSGDILPEILTGNGPS"
BASE COUNT          241 a           93 c          119 g          191 t
ORIGIN      
        1 tatgagatgg aaaatgatgc ccccccaaaa atactaattc caattgacca taatttaaat
       61 tttattgatg caaaatataa tggagaaact tatcggggaa atgaagagtt tgctattaat
      121 ctttttctgc agcatatatt acataatgac atacaagaac aaacatcgat agacttatac
      181 acgagcataa taaataaaga gttggatagc aatagaaaat catacaataa tgaaattttt
      241 aacaatttct cttttgataa gtctgtaaag ttgaattcat ataactatat tgcagatgat
      301 atagagcaag taatcgataa aggaagcaaa gttcaattgg aggtatataa tttattatcc
      361 gaagaaaaga tatttgaaca taaaattatg aataattgga caaggagcat aaaaaatata
      421 ttgacgacat atttgtttat gtcatcagga gcggtgacag ccagaaatgt tcaaaccttt
      481 tctccaacaa taaataatga gtcaaggatt cgattgccga gagcattgcc agtaggccat
      541 ccatatcctg aggaacataa ggcttctggt ttctcccctt ttatgatggg ggggctgagt
      601 ggtgatattc ttccggaaat tttaacgggg aatggaccat ctat
//
LOCUS       DAZKFZ010000558          643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000558 DAZKFZ010000000
VERSION     DAZKFZ010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.562"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..389
                     /locus_tag="ACHOQW_005229"
     CDS             <1..389
                     /locus_tag="ACHOQW_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9576077.1"
                     /translation="DQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDF
                     RLYCEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYR
                     QQYQYYFADKQMSARGAYEYRCTTMG"
     gene            361..>643
                     /locus_tag="ACHOQW_005230"
     CDS             361..>643
                     /locus_tag="ACHOQW_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9576078.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAE"
BASE COUNT          186 a          120 c          181 g          156 t
ORIGIN      
        1 ttgatcaacg ggcgctgggc gttaaccggg tgaagatatt cgaacgctgg aaagctatcg
       61 acaccagaga taagcgtgac aaattcacgg cgctgattcc ggcaattatg gaggctatcc
      121 ggatcagcga tttcagattg tattgtgaaa ttactgacgg aaaaagcatt acgtacatga
      181 tcgccgggtt aaacaaagaa tatggcgatg tggtggagtc cgggctgctt tttgcggatc
      241 cagttgttgt ggaacgtgag actgacgagc ttatagaaaa agctattgct ttcaagcacg
      301 cgtatcgtca gcaataccaa tattactttg cagataaaca aatgtctgcc aggggggcgt
      361 atgagtatcg atgcactacg atgggctaaa aaggtgaaaa ccggcagttc atccagtaag
      421 tcagtattga cctggcttgc tgatatgtgc ggtgccgatt tgtgtgcata cccgtctgta
      481 tctgcactgg cagaagtaac ggaactgaac aaaaagactg tgcaggacag cttacgacac
      541 ctgatggaga ttgggttaat tgttgatacc ggtgagagaa aaggcagaac aaagcaaatt
      601 gtggtgtacc gacttatcgg tgtagaagaa agtgttgccg agc
//
LOCUS       DAZKFZ010000559          642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000559 DAZKFZ010000000
VERSION     DAZKFZ010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.563"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>642
                     /locus_tag="ACHOQW_005231"
     CDS             <1..>642
                     /locus_tag="ACHOQW_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9576079.1"
                     /translation="VVKGVRFSLRLTSGSGQDSRLVTTAITADTEHRFSGLPLGEYTL
                     TVRAINSYGQQGEPAITTFRINAPAKPATIELTPGYFQITAVPVLAVYDPTVQFEFWF
                     SEKRITNTAQVEKSARYLGSGSQWTVQGSRIKPGTDFWFYVRSVNLVGKSAFVEVSGQ
                     PSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEIT"
BASE COUNT          158 a          157 c          203 g          124 t
ORIGIN      
        1 ggtggtgaag ggcgtgcgct tcagtctgcg cctgaccagt ggaagcggtc aggacagccg
       61 tctggtgacc accgccatca ctgcggatac agagcatcgt ttcagtggtc tgccgctcgg
      121 ggaatacacc ctgacagtca gggcaattaa cagttatggc cagcaggggg aaccggccat
      181 caccaccttc cggattaacg cgccagcaaa acccgccacc attgaactga cgccggggta
      241 ttttcagata acggcggtac cggtgctggc ggtgtatgac ccgacggtgc agtttgagtt
      301 ctggttttcg gaaaaacgca tcacgaacac ggcacaggtg gaaaaatctg cccgttatct
      361 ggggagcggc agtcagtgga ctgtccaggg aagccggatt aagccgggga cggatttctg
      421 gttttacgtg cgcagcgtca acctggtggg gaaatctgcg tttgtggaag tcagcgggca
      481 gcccagcaat gatggtgaag ggtatctgga atttttccgg gaaaaaatag gaaaactgca
      541 tctggctcag gggctatggg agctgataga caacagccag cttgcggatg agatggcgga
      601 gatgaagacc accatcacgg aaacccgcaa tgaaatcaca ca
//
LOCUS       DAZKFZ010000560          634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000560 DAZKFZ010000000
VERSION     DAZKFZ010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.564"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            74..>634
                     /gene="escD"
                     /locus_tag="ACHOQW_005232"
     CDS             74..>634
                     /gene="escD"
                     /locus_tag="ACHOQW_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP9576080.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCESTT"
BASE COUNT          178 a          134 c          131 g          191 t
ORIGIN      
        1 tctattctta gacgatgtaa gttcaccata ttttttctca ttgttcaacc accaggatga
       61 ataaaattta aaaatgttat cctcatataa aataaaactt ctcaatggtg cgatgcgtaa
      121 tcgggaatta caacttccga tgggaaacct aacgctaggt acggaagata acgatattgt
      181 ttactttccc cttgaacaag gcctcaatca gtttttactt gatatacgtg aggaaggtgt
      241 tttcctttta tctccggttg aattttggat tgatgggcaa cccacgccct atgaagcaga
      301 taaaccatta cctgtcggaa aggttattga tatagcggga tgttgcttca tcattggtga
      361 tattgaccat tccttgccat tgtctgatgt gccagaaagg ttctctgcca aaagtcgacg
      421 caaaaaacgc cttatcctgg cgagcgtgat tggtgctact tttgcgttgt ctggtgccat
      481 cggcagttac gttttgcttt ccccaaaagc tgaaccccca acgtttacca gagctgatgt
      541 atatcagcag ctaaaagaaa acaaacttca tgcaatcact ctggtatggc atgggaaaaa
      601 tatcgcgctt tatgggcgtt gcgaatcaac cacg
//
LOCUS       DAZKFZ010000561          623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000561 DAZKFZ010000000
VERSION     DAZKFZ010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.565"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>623)
                     /locus_tag="ACHOQW_005233"
     CDS             complement(<1..>623)
                     /locus_tag="ACHOQW_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9576081.1"
                     /translation="TAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLT
                     AVVSPDGLESRRAYDEPGRLVSETSRCGDVIRYAYDNPHSELPATTTDATGSTRQMTW
                     SRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHE
                     TRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           96 a          211 c          172 g          144 t
ORIGIN      
        1 aggtcatact ccatactgcg cgtcagcccg ccctgcgtgg tgctgaccgc ttttccccac
       61 gcatcgtact gtgtctcgct ccggttgccg tccggagtga taacggcagt caggtcgcct
      121 gcggcgttgt actcataccg cgtttcatgg ccctgtgcgt ctttcaccga ggttaaccgg
      181 ccacggttgt catagcggcg gtaacggctg ataccttcct cacggtggac cgccgtcatc
      241 tgaccaaagc ggtcgtattc ataacgggtc tggtagcccg agcagtcggt gaacgccagc
      301 aactgcccgt agcggctcca ggtcatctgc cgggtgctgc ccgtcgcatc tgttgtcgtg
      361 gccggtaatt cactgtgcgg attatcataa gcataccgga tgacgtcccc acagcgcgat
      421 gtctccgata ccagcctgcc cggttcatca tatgcccggc ggctctccag cccgtccggg
      481 gacaccaccg ccgtcagctg gttcccgtcg ttatagtaaa atttcgtctc ccgcccgtcc
      541 ggtgtggtga tgtccgtgat atcgccggac accacattca gaccgtactc tgtccgccgt
      601 cccgccgcgt ccgtctgcgc cgt
//
LOCUS       DAZKFZ010000562          623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000562 DAZKFZ010000000
VERSION     DAZKFZ010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.566"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>623
                     /locus_tag="ACHOQW_005234"
     CDS             <1..>623
                     /locus_tag="ACHOQW_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9576082.1"
                     /translation="ALDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESD
                     RLTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPE
                     TGELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDR
                     LHRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNGD"
BASE COUNT          147 a          179 c          207 g           90 t
ORIGIN      
        1 tgcgcttgac cggctggtac agcagcgcgg ctttgacggg cggacgcaac gttaccacta
       61 tgacctgacc ggaaaactca cgcagagtga agatgagggg cttgtcaccc tctggcacta
      121 cgacgaatcg gaccgcctca ctcaccgcac ggtgaacggc gaaccggcag agcagtggca
      181 gtacgacgag cacggctggc tgacagaaat cagccacctg agcgaaggcc atcaggtggc
      241 ggtgcattac ggttatgatg ataagggccg cctggccggg gagcgccaga cggtgcataa
      301 cccggagacg ggggaactgc tgtggcagca tgagacagag cacgcataca acgaacaggg
      361 tctggcaaac cgcgtcacgc cggacagcct gccgcgggtg gagtggctga cctacggcag
      421 cggttatctt gcggggatga agctgggcgg gacgccgctg gtggagttca cgcgcgacag
      481 gctgcaccgc gagacggtgc gcagcttcgg caataacgca tacgaactga ccagcacata
      541 cactcccgca ggccatttac agagccagcg cctgaacagc caggtgtatg accgtgacta
      601 cgactggaat gacaatggcg acc
//
LOCUS       DAZKFZ010000563          612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000563 DAZKFZ010000000
VERSION     DAZKFZ010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.567"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            52..>612
                     /locus_tag="ACHOQW_005235"
     CDS             52..>612
                     /locus_tag="ACHOQW_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9576083.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          145 a          155 c          174 g          138 t
ORIGIN      
        1 tgagctcatt actgacagta aggcgctgag ggaagagctg gagacaatgc tgtgacttca
       61 ccatttattc aacaaattgc tgataaccgg gtatgtcagg tactcacctg tcttcctgaa
      121 aaatttgtgg ttgatttcgc aaacggaatc gatgttgcac aggagcatat ccgcacggcc
      181 ggagagcgca cgttcttccg gcgtttaaaa gaggggctga ccggcgaagg cgctgcacgg
      241 cagaatgcca tcaatgcgtc gcttgcacag ggcgttgagg cgtccctgcg ctggctgacg
      301 gagatgacaa cgtcacttgc caccacaaac tacgcgatta cccgggtaaa cgacagggtc
      361 agttcactgg tcagtgatac agccaggctg gcgcattatt ccgcagatac gcgggagcag
      421 ttactcaccc tggccgatca ggttcaccat aaactgaatc atcttgaaga aaaactccac
      481 cgtgttgacc aggttcagcg ggcgcagtta catcttgagc agatattctc atggtggagc
      541 gccgggcgat atgcgtcatt ttcccctgcc ggacgttgtt atgtggcact tgaagagctt
      601 cgctggggcg cg
//
LOCUS       DAZKFZ010000564          610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000564 DAZKFZ010000000
VERSION     DAZKFZ010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.568"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            2..532
                     /locus_tag="ACHOQW_005236"
     CDS             2..532
                     /locus_tag="ACHOQW_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9576084.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRILLNR
                     VDTTVAADVEWPVAPQ"
BASE COUNT          171 a          126 c          162 g          151 t
ORIGIN      
        1 tctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatacattc cgcctcatac
       61 aggtctgcca gcaaacagta ccgatattgc accgccagat attccggctg gcttcgtggc
      121 tgttttcaac agtaatgagg catcgtggca tctcgttgaa gatcatcggg gtaaaacggt
      181 ttatgacgtg gcttccggcg acgcgttatt tatttctgaa ctcggtccgt taccggaaaa
      241 tgttacctgg ttgtcgccgg atggagagtg tcagaagtgg aacggcacat cctgggcgaa
      301 agatgcagaa gcagaaaaac tgtttcgggt acgggaggca gaagaaacaa aaaacagcct
      361 gatgcaggta gccagtgagc atattgcgcc acttcaggat gccgtagatt tggatattgc
      421 gacggaggaa gaggcatcgt tactggctgc atggaagaca tatcggatat tgttgaatcg
      481 tgttgataca acagtagcag cggatgttga gtggccagtc gccccacaat aaaaagaaaa
      541 agccatcgac agaaatatcg atggctttat gtactctatt tatacaatac aacaccactc
      601 tttttagtta
//
LOCUS       DAZKFZ010000565          608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000565 DAZKFZ010000000
VERSION     DAZKFZ010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.569"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..71
                     /locus_tag="ACHOQW_005237"
     CDS             <1..71
                     /locus_tag="ACHOQW_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9576085.1"
                     /translation="RAIKAEYQQKLKKLRNSRSEVA"
     gene            68..>608
                     /locus_tag="ACHOQW_005238"
     CDS             68..>608
                     /locus_tag="ACHOQW_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9576086.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGAD"
BASE COUNT          201 a          119 c          153 g          135 t
ORIGIN      
        1 gcagggccat caaggcggag tatcaacaga aacttaaaaa actgcgaaac agcagaagtg
       61 aggttgcatg aatatctacg aaagaattga tggcagcaaa taccgaaata tttgggtagt
      121 tggcgatctg cacggatgct acacgaacct gatgaaaaaa ctggagacga taggattcga
      181 caccaaaaaa gacctgctta tctcggtggg cgatttggtt gatcgcggta cagagaacgt
      241 agaatgcctg gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca
      301 aatgatgatt gatggcttat cagagcgtgg aaacgtcaat cactggctgc ttaatggcgg
      361 tggctggttc tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa
      421 agcagatgaa cttccgttaa tcatcgaact ggtgagtaaa ggaaaaaaat atgtcatctg
      481 ccacgccgat tatccttgtg ataaatacga gtttggaaag ccagttgatc atcagcaggt
      541 aatctggaac cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaatcaaagg
      601 cgcggaca
//
LOCUS       DAZKFZ010000566          608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000566 DAZKFZ010000000
VERSION     DAZKFZ010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.570"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>608
                     /locus_tag="ACHOQW_005239"
     CDS             <1..>608
                     /locus_tag="ACHOQW_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9576087.1"
                     /translation="LRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKA
                     LSNFEECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDA
                     DYDHDRIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQ
                     GCKDYLAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIK"
BASE COUNT          191 a          119 c          155 g          143 t
ORIGIN      
        1 gctgcgtgtt gagcttgaag cagcaaaatc aaaactcaac gagcagcgtg aatattacga
       61 gggagtaatc gcggatggaa gtaagcgcat agcagaactg gaaaaacaat gcgccgaatg
      121 ggagcgaaaa gcattaagca actttgaaga gtgtgctgcg atggctgaac gtatcgaaga
      181 gatgcagaca aaatctgcac cagattcgtt tggcatcatc ggtgaaaata ttcgaacaca
      241 ggacaatcga ataacgtcag atcccatgtt ttgtgtgtat caaaagcgcg aaatcgctgt
      301 tgatgctgat tatgaccatg accggattgt ctgggttgac gaagatggca atgaagccaa
      361 taaacgccat agtcgtcgtc tcgagctact tcatgaaaac tttcgagagc caccagaaaa
      421 atggcggcgc gttgctgtga aagatattga tgaattcgtt acctgctgtt tcaccgaaca
      481 gggttgtaaa gactacctgg cagtcaatgg tcacaatctt cgcttgccat ttatatatgt
      541 aaaaagcggt ttcaggaacg ctgaatatat cggcataaga aactggcttg ctggcattcg
      601 catcaaag
//
LOCUS       DAZKFZ010000567          607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000567 DAZKFZ010000000
VERSION     DAZKFZ010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.571"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>607
                     /locus_tag="ACHOQW_005240"
     CDS             <1..>607
                     /locus_tag="ACHOQW_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP9576088.1"
                     /translation="DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAF
                     EEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCEVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPKMTVRYYISSADLTAGKFATAIRNHWHVENKLHWRLDVVMNEDDC
                     KIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMD"
BASE COUNT          218 a           99 c          152 g          138 t
ORIGIN      
        1 ggatattaaa ggaaaaatca tcacaactga tgcgatgggt tgccagaaag atattgcaga
       61 gaagatacaa aaacagggag gtgattattt attcgcggta aaaggaaacc aggggcggct
      121 aaataaagcc tttgaggaaa aatttccgct gaaagaatta aataatccag agcatgacag
      181 ttacgcaatt agtgaaaaga gtcacggcag agaagaaatc cgtcttcata ttgtttgcga
      241 agtccctgat gaacttattg atttcacgtt tgaatggaaa gggctgaaga aattatgcgt
      301 ggcagtctcc tttcggtcca taatagcaga acaaaagaaa gagccaaaaa tgacggtcag
      361 atattatatc agttctgctg atttaaccgc agggaagttc gccacagcaa tccgaaacca
      421 ctggcacgtg gagaataagc tgcactggcg tctggacgtg gtaatgaatg aagacgactg
      481 caaaataaga agaggaaatg cagcagaatt attttcaggg atacggcaca ttgctattaa
      541 tattttgacg aatgataagg tattcaaggc agggttaaga cgtaagatgc gaaaagcagc
      601 catggac
//
LOCUS       DAZKFZ010000568          605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000568 DAZKFZ010000000
VERSION     DAZKFZ010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.572"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>605
                     /locus_tag="ACHOQW_005241"
     CDS             <1..>605
                     /locus_tag="ACHOQW_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9576089.1"
                     /translation="KTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESR
                     YLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQ
                     PGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEIL
                     ADRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHC"
BASE COUNT          138 a          176 c          199 g           92 t
ORIGIN      
        1 aaaaccgacc tcatcccgga aggggttatc cgcacggatg atgagcggac tcaccggtac
       61 cattacgaca gtcagcaccg gctggtgcac tacacgcgga cacaatatga agagccgctg
      121 gtcgaaagcc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg ggtgtggcga
      181 cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccgcaagt gacctggtac
      241 ggctgggacg gcgaccggct gaccacaata cagaacgaca gaacccgcat ccagacgatt
      301 tatcagccgg ggagcttcac gccactcatc agggttgaaa ccgccaccgg tgagctggcg
      361 aaaacgcagc gccgcagcct ggcggatgcg cttcagcagt ccggcggcga agacggtggc
      421 agtgtggtgt tcccgccggt gctggtgcag atgctcgacc ggctggaaag tgaaatcctg
      481 gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct gactgtggcg
      541 cagatgcaaa gccagatgga cccggtatac acgccggcgc gaaaaatcca cctgtaccac
      601 tgcga
//
LOCUS       DAZKFZ010000569          599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000569 DAZKFZ010000000
VERSION     DAZKFZ010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.573"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            271..>599
                     /gene="tccP"
                     /locus_tag="ACHOQW_005242"
                     /gene_synonym="espF(U)"
     CDS             271..>599
                     /gene="tccP"
                     /locus_tag="ACHOQW_005242"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFP9576090.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAP"
BASE COUNT          188 a          138 c          101 g          172 t
ORIGIN      
        1 cgcttaagag tagatttttg gcattttggg aagatcagcg ggacataaga gacatacaac
       61 acaggaggaa tggctgtttt ttaatagtgc tttgaattcc gcatgaaacc tttcttagca
      121 gtcgaaaagc aaaacatacg ttttcttttt aacaataatt gatgagtatt gcatcgagtg
      181 tcatttatac atcgccgcat ttatttgcca ctataaaaag gctgattaca tatctatcgg
      241 agaactaaaa ttacttataa ggtaatctgc atgattaaca atgtttcttc actttttcca
      301 accgtcaacc gcaatattac agctgtatat aaaaaaagca gcttctctgt atcaccacag
      361 aaaatcacat taaatcctgt aaaaatcagc tcaccttttt caccaagcag tagctccatc
      421 agcgcaacaa ctctctttcg agccccaaac gcccattcgg catcatttca tcgacagtct
      481 actgctgaaa gttcgttaca tcaacaactt cctaatgtga ggcagcgcct gatacaacat
      541 cttgcagagc atggcattaa acctgcccgg agtatggctg aacatattcc tccggcacc
//
LOCUS       DAZKFZ010000570          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000570 DAZKFZ010000000
VERSION     DAZKFZ010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.574"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            27..581
                     /gene="ybeT"
                     /locus_tag="ACHOQW_005243"
     CDS             27..581
                     /gene="ybeT"
                     /locus_tag="ACHOQW_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9576091.1"
                     /translation="MNKKLMYIFTIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
BASE COUNT          194 a          129 c          123 g          147 t
ORIGIN      
        1 taaaaagaaa atcgaagcat tcacacatga ataaaaaatt aatgtatata ttcacaattt
       61 ttatagttgc agcaattacc tgtattagcc aacccaagaa aacgacgttg cgtgataaag
      121 ccatggtgaa ttatgccttt gattatttaa gctcaccggg cagtcttcca ttcaccacgg
      181 cagccacgga gctttccgcg attcatggtc actcaacgtc gcaatatcgc cttggagaat
      241 tttatcttca tggtagcgac ggtaaaccac tggattatac acaggcgaga tactggtatg
      301 agcaatcagc ggaacaggaa aatccacgcg cgcaaagtaa actggggtgg atctacctca
      361 aaggtctggg ggtcaaaccc gacacccgta aagcaattct ctggtataag gaagcagctg
      421 aacaagggta tgctcatgct caatatactt taggtttgat ctacagaaat ggctcaggta
      481 ttaatgttaa ccattatgaa tctcaaaaat ggttaaaact ggccgccaaa caacattaca
      541 aaaatgcgga aagattactt gccgggcttc ccgcacatta acgtcgactg tca
//
LOCUS       DAZKFZ010000571          578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000571 DAZKFZ010000000
VERSION     DAZKFZ010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.575"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>578
                     /locus_tag="ACHOQW_005244"
     CDS             <1..>578
                     /locus_tag="ACHOQW_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576092.1"
                     /translation="IESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAG
                     RINARLEDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVC
                     RWFGTSDLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEI
                     DAFFCAFREKVEQLRNTLIQSSEDHKRDQQAQ"
BASE COUNT          152 a          140 c          171 g          115 t
ORIGIN      
        1 aatcgaatcg accgtgacca gcctgcaccg tgaggctgaa gcacagttcc ggcctgagct
       61 ggagaaaatc gtcagcggga ttgaaacagg ttttcgtggc acggccctgt acgccacaga
      121 aaacattgcc ggtcgcatca atgcccgcct ggaggatgag ggctttaccg taaaaatcag
      181 ttttccggca gtcagccagt tacagacccg gctcgcggta aaaataaatc tgagtgcgct
      241 tatggaggaa agaacggaga ccgtcacccg tcgccgtcgg cagagtggcg tatggggaac
      301 cgtttgtcga tggtttggca ccagtgacct gggctgggaa aactatgacg aggatgtgag
      361 tcgcagcgtg atcaatatca acaaggtcag agaggaagtt atgtcactga cccgggcata
      421 tttcggggag ctgcaggcat ccattgagca ggatattaac cagcccgtcc gccaggaaat
      481 cgatgccttt ttctgcgcat tcagggagaa agttgaacaa ctgcgtaaca cgctgattca
      541 gagctctgaa gatcataaac gcgatcagca ggcgcagg
//
LOCUS       DAZKFZ010000572          578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000572 DAZKFZ010000000
VERSION     DAZKFZ010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.576"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..150)
                     /locus_tag="ACHOQW_005245"
     CDS             complement(<1..150)
                     /locus_tag="ACHOQW_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9576093.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKA"
BASE COUNT          193 a          141 c           72 g          172 t
ORIGIN      
        1 cgccttacgc aaacactctg ctcgtttctg tgccgccact tttcttttct ggtcatcact
       61 tagctgtacc atcacttttg cccatcgttc agctgctctc cggtacagtc cttttttctc
      121 cagacattct gccaggtgat catgtagcat aagtaacctc cgtttatcta cagactgcca
      181 tcctgaattt accgtcctta ataagacaac aataaaaaac taattatata aaaacaataa
      241 aaataatata caaaaaaact aaacaacaaa gaaaagcaaa tcatatctgg cattttaatt
      301 gaaagagcaa ttaccgaaca aaagacgctg actatatact caaaaccaaa caacaattct
      361 gccaatcagg tatcatggca acacacggaa ttaccatgtt tttgccttct ctgcccatac
      421 aatacgggca tatacttcat actctattgt aatatttcta tccatgtgcc ccactccatt
      481 tacctgtaaa taatattcaa aatatttatc acagaaatcg tttttggcca tgaactgagc
      541 acactataaa gtccggaact gactctttgt taaattac
//
LOCUS       DAZKFZ010000573          570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000573 DAZKFZ010000000
VERSION     DAZKFZ010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.577"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(125..547)
                     /locus_tag="ACHOQW_005246"
     CDS             complement(125..547)
                     /locus_tag="ACHOQW_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576094.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
BASE COUNT          171 a           83 c          124 g          192 t
ORIGIN      
        1 ttagctcttt taatttttcg taaaaaagag aggtacatga aggtgctatc agtatgagta
       61 acgttaatca aaggttttat tacgtctggc aataacgcag gccatttttc gcgacagggt
      121 ggatttaatc agtataatcc tcatacaata gttcacgatt aactgaaccg ttaacgggga
      181 tttggtgttg aaatttagaa atggcggagt caattaatga agacaatatc gaaaaaatga
      241 ctgtgtcaga attaatttca attaatcctt ctcgttgttg accaaaaaag atatttatct
      301 tgatttcatt tgtactacgg caatcaatat gtagtaattt ttgatcgctg agattttctg
      361 tttcataaat gtttttggcg agagtcgggt gtattgatgc cagacatctg ctcagataag
      421 ccatatcctt tagggaacac cccgctgagt agaggatgtc agagaatttt tgtgtgtcaa
      481 ctgttgtctc tgaagcgtat gttgcattga ctggaagggt attgaaagag atctgagatg
      541 gtatcattat ttaaatcttt ccgtgagtaa
//
LOCUS       DAZKFZ010000574          556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000574 DAZKFZ010000000
VERSION     DAZKFZ010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.578"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..556
                     /gene="ygeQ"
                     /locus_tag="ACHOQW_005247"
                     /pseudo
     CDS             1..556
                     /gene="ygeQ"
                     /locus_tag="ACHOQW_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YgeQ"
BASE COUNT          184 a          116 c          125 g          131 t
ORIGIN      
        1 tcttctgatt cactcagcca aattacaaaa tgaaataaag agccactata ccaaagacga
       61 aattcaagga ttgctaacat taacagaaaa cacacgaaag ttaacgttga cggagaaacc
      121 ctggggaacg tttattcttg cttctacttt cgaagatgac aaaacagcgg cagaaaccca
      181 ctatgatgct gtctggctta gggatagttt atggggctat atggcattag tatcagacca
      241 gggaaatagt gtagccgcaa aaaaggttct gctcaccctg tgggattata tgtctacgcc
      301 cgaccagatt aagcggatgc aggacatcat tagcaaccca aaacgactgg atggtattcc
      361 cgggcaaatg aatgctgtgc atatttgaca gcaactcccc cgtgatggca gatgtgcagg
      421 aagaaggcaa gccgcagctg tggaaccata aacaaaatga tgcgttaggt ctttatcttg
      481 atctccttat acaagcaatc aatactggta ctattaacgc tgaagattgg caaaaaggtg
      541 accgactaaa atcggt
//
LOCUS       DAZKFZ010000575          555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000575 DAZKFZ010000000
VERSION     DAZKFZ010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.579"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..406)
                     /locus_tag="ACHOQW_005248"
                     /pseudo
     CDS             complement(1..406)
                     /locus_tag="ACHOQW_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          185 a          106 c           80 g          184 t
ORIGIN      
        1 gtagaaataa tgctattttc aataatgcct ggcaatgaat actgccaaac ttcatttttt
       61 cttttacagc aaatttgaga cctaaaactt ctatttgctg attcaatgtt aacgttctat
      121 cttcaggttc ttgtgctgtt aattctctga ttgttacaac cagatcaggg tcggtagtga
      181 ttataatctc aaaactacct gtaagctcgc cataaggaac gactattgta ttttctggta
      241 actcagaaaa agcatcactt cttcctaacg aaacatcatt accaatgata acaagtagac
      301 gcccattaag tatttcatac tcgcatccat tctatggtcc gctcaacaaa cgaatggcat
      361 aagattccaa taagtcttgt gataagaatt tatcattatt ttccatgaat accacccgct
      421 aattccataa gctctagttt agagtagtgt aacaagacgg aaatttcatg accaaattat
      481 gatgagacag aataaaaaaa ttataatttt gaggaccatt ttaaatgcct ctatgcataa
      541 ctttatttac aattt
//
LOCUS       DAZKFZ010000576          552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000576 DAZKFZ010000000
VERSION     DAZKFZ010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.580"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>552)
                     /locus_tag="ACHOQW_005249"
     CDS             complement(<1..>552)
                     /locus_tag="ACHOQW_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9576095.1"
                     /translation="DSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQAV
                     FYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKV
                     EWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGV
                     YTSANLSAYSNSIVDTPTYFAFA"
BASE COUNT          109 a          176 c          125 g          142 t
ORIGIN      
        1 tttgcaaacg caaagtacgt gggcgtgtcc acaatgctgt tgctgtacgc gctcaggttt
       61 gccgaggtat acacgccgcc ttttgcattg gccaccgctc cgcccagcca gtcaccaatg
      121 ctgccgagaa atcctcccgc accggacata ccgtttgccg ccgtcttaat tccgttgaca
      181 atcgcggcat tcataagaac ttttgatatt tcctgcagca ctgatgaggc ccagctgcgc
      241 cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc tgagataccc
      301 tccgtcgcca gctgtgctgc ctgtgaggcg taatcggacg cattatccac ccagttactg
      361 aatccctcct gcagcccttt ctgccagtcc gcacgctgca catccgattc ggcataaaag
      421 actgcctggt ccttaaggcg ttcgctcaga tactgcgcgt tctgtgccag agcctgtctg
      481 taaaaatcct cactgatatc cccggtctga tactgagact gaaggtccgc atccttctgg
      541 cggaagctgt cg
//
LOCUS       DAZKFZ010000577          541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000577 DAZKFZ010000000
VERSION     DAZKFZ010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.581"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>541
                     /gene="nleH1-2"
                     /locus_tag="ACHOQW_005250"
     CDS             <1..>541
                     /gene="nleH1-2"
                     /locus_tag="ACHOQW_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFP9576096.1"
                     /translation="SLTRNLTSPDNRVLSSVRDAAVHSDSGTQVTVGNRTYRVVVTDN
                     KFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTIERGSVHSNRPDLPP
                     VDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYEDMEDTTKVLKMFTIS
                     QSHEEVTSEVRCFNQYYGSG"
BASE COUNT          157 a           96 c          136 g          152 t
ORIGIN      
        1 attctttaac cagaaacctg acttcgcctg ataatcgtgt tttatcctct gtaagggatg
       61 ctgctgttca ctctgatagc gggacgcaag taacggttgg caacagaaca tatcgtgttg
      121 tggtcactga taataagttt tgcgttacaa gagaaagtca tagtggttgt tttactaatc
      181 tgttgcacag gttgggatgg cctaagggag agattagcag aaaaattgag gctatgctga
      241 atacatcgcc agtgagcacg actatagaaa gaggctctgt tcattcgaac agacctgatt
      301 tacctccagt ggattatgcg cagccggagt tacctccagc ggattatact caatcagagt
      361 tgccgagggt tagcaacaat aaatcacccg tgccaggtaa tgttattggt aaaggtggta
      421 atgctgtcgt gtatgaagat atggaagata caacaaaagt gttgaagatg tttactatat
      481 ctcaaagcca tgaagaggtg acaagcgaag ttcgttgttt caatcagtat tatggttccg
      541 g
//
LOCUS       DAZKFZ010000578          531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000578 DAZKFZ010000000
VERSION     DAZKFZ010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.582"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..310)
                     /locus_tag="ACHOQW_005251"
     CDS             complement(<1..310)
                     /locus_tag="ACHOQW_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9576097.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     E"
     gene            complement(307..528)
                     /locus_tag="ACHOQW_005252"
     CDS             complement(307..528)
                     /locus_tag="ACHOQW_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9576098.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVPGCRTCASCQEDLELISKQRGSK"
BASE COUNT          120 a          141 c          103 g          167 t
ORIGIN      
        1 cctcgttctc ctggtcgcgg cgtttgatgt attgctggtt tctttcccgt tcatccagaa
       61 gcgccagcgc aacatttgga ttaaaggcag caataaattc agcgtttgca taagcctgaa
      121 catctgtttc aaccaggcag ttaacatgac attccgcaat cacaccaccg ggttctcctt
      181 tccatttttg gcaaacaaaa actcctgtta aattgccgtg ctggttaaca gatgtatgcc
      241 ctacgatgta gcttccttta gttgctttct ctgccttttc acgcagtgcc tgataattaa
      301 tttcgctcac ttcgaacctc tctgtttact gataagctcc agatcctcct ggcaacttgc
      361 acaagtccga caacccggaa ctgccaggcg tcttcgttca tctatcggat cgccacactc
      421 acaacaatga gttgcggata cagtctggta gttcaggcga cgcattttta ttgctgtatt
      481 gcgctgtaat tcttcaattt ctgatgctga atcaatgatg tctgccatct t
//
LOCUS       DAZKFZ010000579          531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000579 DAZKFZ010000000
VERSION     DAZKFZ010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.583"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..310)
                     /locus_tag="ACHOQW_005253"
     CDS             complement(<1..310)
                     /locus_tag="ACHOQW_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9576099.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLELLDERERNQQYIKRRDQEN
                     E"
     gene            complement(307..528)
                     /locus_tag="ACHOQW_005254"
     CDS             complement(307..528)
                     /locus_tag="ACHOQW_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9576100.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT          112 a          142 c          105 g          172 t
ORIGIN      
        1 cctcgttctc ctggtcgcgg cgtttgatgt attgctggtt tctttcccgt tcatccagca
       61 gttccagcac ggtagccggg ttagcctctg ctatgaattc agcgtttgca taagcctgag
      121 catctgattc aatcaggcag ttaacatgac attccgcaat cacgccaccg ggttctcctt
      181 tccatttttg gcaaacaaaa actcctgtta aattgccgtg ctggttaaca gatgtatgcc
      241 ctacgatgta gcttccttta gttgcttttt ctgccttttc acgcagtgcc tgataattaa
      301 tttcgctcac ttcgaacctc tctgtttact gataagctcc agatcctcct ggcaacttgc
      361 acaagtccga caaccctgaa ctgccaggcg tcttcgttca tctatcggat cgccacactc
      421 acaacaatga gttgcggata cagtctggta gttcaggcga cgcattttta ttgctgtatt
      481 gcgctgtaat tcttcaattt ctgatgctga atcaatgata tctgccatct t
//
LOCUS       DAZKFZ010000580          528 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000580 DAZKFZ010000000
VERSION     DAZKFZ010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.584"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..503
                     /gene="espW"
                     /locus_tag="ACHOQW_005255"
     CDS             <1..503
                     /gene="espW"
                     /locus_tag="ACHOQW_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP9576101.1"
                     /translation="DNAKPNLGERDVRMFENISSDDTDKITGRKFSELGEYLYSGNVI
                     KLSQLSIRYLPNISSISLIETKQSLLLHRLYSDEVLQRNGTLIPTPLHEEKSIPADNI
                     KTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSPRNYGLHHNIKTW
                     DPLVRN"
BASE COUNT          200 a           99 c           92 g          137 t
ORIGIN      
        1 aagataatgc aaaaccaaac ttaggagagc gagacgtaag aatgttcgaa aatattagtt
       61 ctgatgatac agataagata acaggaagga aattttcaga gttaggtgag tatctttatt
      121 cagggaatgt aataaaactc agtcagttat caattcgtta cttacccaat atcagctcaa
      181 tctcattaat agagacaaaa cagagtttgt tgctacatcg attgtattca gatgaagtac
      241 ttcagagaaa tggaacgctt atcccgacac cactacatga agaaaaatca attccagctg
      301 acaatataaa aacaatgctc aacaacatac cgacttacaa aatgttaccg ccattcacag
      361 aaacacaagg taattgttct tctggcgcag ccacgttttt acgcaaatca ggcgccgaag
      421 aaaaagatat tcttgcatgt agcccccgaa attatgggct gcatcataac ataaaaacat
      481 gggacccctt ggttagaaat taaggatcca aatattatat atttcata
//
LOCUS       DAZKFZ010000581          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000581 DAZKFZ010000000
VERSION     DAZKFZ010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.585"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(82..339)
                     /locus_tag="ACHOQW_005256"
     CDS             complement(82..339)
                     /locus_tag="ACHOQW_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576102.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
BASE COUNT          149 a          143 c          100 g          134 t
ORIGIN      
        1 attttgcgcc atcggctttc tccggtggca cagtgttact caacaggggt tcagccctgc
       61 gctgaattgt agatgaattc actaatcttc aaaagcagaa aaaccagcct taatcccagc
      121 ttctttcaga gacggcaacg atgtgacaaa ttcatttgca cgcaaaataa aaccatccgt
      181 cacaagccca tccacaaaat gaattaacgc agctccactc ttcctttgtt gagactgtaa
      241 acatttaata cggcagtggc tgacaatagc gcccttctca acgcgcacag tatagaggcc
      301 atcttcacta aaaatttcac gtaattcttc gattctcatc aacagaatcc ttccagataa
      361 atagcactcc cctgttcggg gtccatccct cttctccctg cgcgctactt aagtattttt
      421 gattctattc cggcgccgtc cggaacttca aacgcgttga aaataaaaac aaaaacccgc
      481 cgaagcgggt taagtgcggg tgcgttgagg atgcctgaca catcag
//
LOCUS       DAZKFZ010000582          525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000582 DAZKFZ010000000
VERSION     DAZKFZ010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.586"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            19..>525
                     /locus_tag="ACHOQW_005257"
     CDS             19..>525
                     /locus_tag="ACHOQW_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576103.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLLKE"
BASE COUNT          178 a          105 c          103 g          139 t
ORIGIN      
        1 taagaaagga catctgaaat gttaaaccgt gacttcgatc atctctcaac gctattacat
       61 ctcttttttg aacgtgagga tatttgccaa aagcttaata ttattgacca aaacaatatc
      121 accggttggg aagtgtggtt ccaggtagag tttgctaata tgctctgctc aacagaccat
      181 gaatggtggc gtgaacaagc actttcctgt gatatgcgca aaaaacctga gcgcccgaca
      241 ctacgcacag atttcttact acgtaaaaaa ggctgggcac aagatagcta tatcgcgctt
      301 gaaataaaac aaaacagaga cgcgaccagt tgtgttaaaa atatgatcgc agacttagaa
      361 aaaagcgcaa aaataaaacg ttccgaactc gatttacgca gtttctggac cttgggtatc
      421 acccctatga tagacaggga aaggctggac tctctcatcg attgctattt agatgagaaa
      481 ttttatctta ctaaatctcg taaaaaacac gttttactta aagaa
//
LOCUS       DAZKFZ010000583          520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000583 DAZKFZ010000000
VERSION     DAZKFZ010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.587"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>520)
                     /locus_tag="ACHOQW_005258"
     CDS             complement(<1..>520)
                     /locus_tag="ACHOQW_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576104.1"
                     /translation="SWTGYDSSLEGHSRLPDIKSVIAIAEIFSKRDEQLSSRDIFTTS
                     VLALLMCAPSRISEILALPADCEITECDGKGIQRYGLRFFSAKGYEGNIKWIPTLMIP
                     VAKKAISRLKELSSQARLLAAEIQKNYSNSTKGTLKENIPPDLFWYDREKKIKYSNAL
                     CLLTEGQLNQNKK"
BASE COUNT          159 a          107 c           76 g          178 t
ORIGIN      
        1 ctttttattt tgatttaact gtccttcagt taacaagcaa agcgcattag aatatttgat
       61 tttcttctct ctatcatacc aaaagagatc aggaggtata ttttctttaa gggttccctt
      121 cgttgaatta gagtaattct tttgaatttc agcagccaat aacctcgctt gacttgataa
      181 ttcttttaat ctgctaatag cctttttagc tacaggtatc attaaagttg gaatccattt
      241 tatattgcct tcataccctt ttgctgaaaa aaatcttaaa ccgtatcttt gaatgccctt
      301 tccatcacat tctgtgattt cacaatccgc tggcaaagct aaaatttccg atatcctact
      361 cggtgcacac ataagtaaag caagcacaga tgtagtaaat atatctcttg acgataattg
      421 ttcatcccgc tttgaaaaaa tctcagcaat cgcaataact gatttgatat caggcaatct
      481 agaatggcct tccagtgaag aatcataacc agtccaagac
//
LOCUS       DAZKFZ010000584          520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000584 DAZKFZ010000000
VERSION     DAZKFZ010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.588"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>520)
                     /locus_tag="ACHOQW_005259"
     CDS             complement(<1..>520)
                     /locus_tag="ACHOQW_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576105.1"
                     /translation="ILQNNKLQPTEIYKESQQTAKLEFFCDEFLKISQSRYGLSTSTD
                     SIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKNEFAADYEPYTLT
                     FTRGENNEGILSVESKEGFISQRTINLNEYETAINIINEHVIKENIHNTVQSPTEKYI
                     SKINSSDKPYKTS"
BASE COUNT          136 a           75 c           97 g          212 t
ORIGIN      
        1 tgctggtttt ataaggttta tcagagctgt ttatttttga aatgtatttt tctgtcggtg
       61 actggaccgt gttgtggata ttttctttta taacatgttc attgatgatg ttaattgcag
      121 tttcatattc atttaagttt attgttcttt gtgaaataaa cccttctttg ctttcgacac
      181 tcaaaatacc ttcgttgttt tcacctcggg taaaggtaag ggtatagggt tcataatccg
      241 ctgcaaattc atttttatac gttatgacat gttcgacaaa atgtcttatt ggtacgaaac
      301 cataactgtg aacgtcaatt ttttgggtct tgatactatc aattgcatct gaggctttcg
      361 tgaaaaaagc caataaatta gctatgctgt ctgttgaggt tgataacccg tatctacttt
      421 gagatatttt tagaaactca tcgcaaaaaa actcaagttt ggcggtttgt tgtgattctt
      481 tgtaaatttc tgttggttgc aatttattat tttgtaaaat
//
LOCUS       DAZKFZ010000585          513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000585 DAZKFZ010000000
VERSION     DAZKFZ010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.589"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..164
                     /locus_tag="ACHOQW_005260"
     CDS             <1..164
                     /locus_tag="ACHOQW_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9576106.1"
                     /translation="SLKIVNGMDSIKLLDKVIIYQQQNEQLYPAQEHLLLQLCMRVTK
                     KLTDILMPP"
     gene            194..478
                     /locus_tag="ACHOQW_005261"
     CDS             194..478
                     /locus_tag="ACHOQW_005261"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9576107.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLKKYGPTLQMMKSKHLK"
BASE COUNT          186 a           80 c           85 g          162 t
ORIGIN      
        1 tatctttaaa aatagttaat ggaatggata gtattaaatt acttgataaa gttattatct
       61 atcaacaaca gaatgagcaa ttatatccag cacaggaaca tcttctttta caactttgta
      121 tgcgagttac caaaaaactt actgatatat taatgcctcc ctgaaggggg atggtataaa
      181 cgatacaacg cttatgcttc tagccttgtt atccagtact gataatttct gcttatcttc
      241 gactgaactc agtgaaagat tagatgtttc cagaacatat atcaccagag cttgtgattc
      301 tctggaaaaa tttggtttta ttaaacgtat ggaaagtaag gaagatcgac gtagtaagaa
      361 tatttatctg acttcagacg gtaatttgta tttacagcgg accacaagga tatatggacg
      421 ctatttaaaa aaatatggac caaccttaca gatgatgaaa tcaaaacatt taaaataatc
      481 agcaaaaagg ttttatctca aaatatttct cag
//
LOCUS       DAZKFZ010000586          510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000586 DAZKFZ010000000
VERSION     DAZKFZ010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.590"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            24..>510
                     /locus_tag="ACHOQW_005262"
     CDS             24..>510
                     /locus_tag="ACHOQW_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9576108.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GK"
BASE COUNT          154 a           98 c          124 g          134 t
ORIGIN      
        1 acgaagagat caggatgata acgatgaaaa aaagtgtatt gacggcgttt ataactgtgg
       61 tatgtgcaac gtccagcgtt atggctgctg atgataatgc tatcacggat ggctcagtaa
      121 catttaatgg taaagttatt gctccagctt gtaccctggt agctgcgacg aaagattccg
      181 tggtgacttt gccagatgtt agtgccacga agttgcaaac caatggtcag gtttctggcg
      241 tgcaaactga tgtgccaatt gaattaaaag attgtgatac taccgtaaca aaaaatgcaa
      301 cgttcacctt taatggcact gcggatacta ctcagattac agcgtttgct aaccaggcct
      361 catctgatgc tgctacaaac gtggccctgc aaatgtatat gaatgatggt acaacggcca
      421 tcaagccaga cacagaaacc gggaacattt tgttgcaaga tggagatcag acgttgactt
      481 ttaaagttga ttatatcgct acggggaaag
//
LOCUS       DAZKFZ010000587          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000587 DAZKFZ010000000
VERSION     DAZKFZ010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.591"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>508
                     /locus_tag="ACHOQW_005263"
     CDS             <1..>508
                     /locus_tag="ACHOQW_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP9576109.1"
                     /translation="CHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDL
                     ISGWEKRQVMKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQV
                     TTDTDTDKELNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRP
                     SLDFRQRAA"
BASE COUNT          140 a          118 c          155 g           95 t
ORIGIN      
        1 atgtcacggc gagtcactac gtggtcacat tgatgtcacg acggaagatt tagcaagtgc
       61 gcttgatgtg acggaagacg tgattgattc aattttgcat gcaatgcagg ggcgagttct
      121 ggatggcgat cttatttccg gatgggaaaa acgccaggtg atgaaggagg ataacggtaa
      181 tgtttcgcaa accgcaaaat ccccggcaga gcgcaagaga gcgcagcggg agcgggaaag
      241 actgcgggag cagaacactg attgtcacga tgagtcacga cgcgtcacgc atatgtcacg
      301 acaagtcacg acagatacag atacagataa agaattaaac cccacacata acgcgcgcat
      361 gcgcgagagt gctccagcca gtgagtcgaa tggcgcgccg ttgcagacag cagaacctga
      421 atacccggat ggcctgagcg aaccgatcgg gaaatttccg atgactggtg tctggcggcc
      481 gtcgctggat tttcgacagc gggctgca
//
LOCUS       DAZKFZ010000588          506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000588 DAZKFZ010000000
VERSION     DAZKFZ010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.592"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..495
                     /locus_tag="ACHOQW_005264"
     CDS             <1..495
                     /locus_tag="ACHOQW_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9576110.1"
                     /translation="ESALVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVEMLKLQQ
                     RTEYISHREVFRPWGKFDSIDQGERYKVKKIIVKPGEGLSLRMHHHRSEHWIVLSGTA
                     KVTLGDKTKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERY
                     KHED"
BASE COUNT          168 a           85 c          115 g          138 t
ORIGIN      
        1 gagtcagcgt tggtagccgc cattggaatt gaagatatgg ttatcgtgca aactaaagat
       61 gccgttcttg tgtctaaaaa gagtgatgta cagcatgtaa aaaaaatagt cgaaatgctt
      121 aaattgcagc aacgtacaga gtatattagt catcgtgaag ttttccgacc atggggaaaa
      181 tttgattcga ttgaccaagg tgagcgatac aaagtcaaga aaattattgt gaaacctggt
      241 gaggggcttt ctttaaggat gcatcaccat cgttctgaac attggatcgt gctttctggt
      301 acagcaaaag taacccttgg cgataaaact aaactagtca ccgcaaatga atcgatatac
      361 attccccttg gcgcagcgta tagtcttgag aatccgggca taatccctct taatcttatt
      421 gaagtcagtt caggggatta tttgggagag gatgatatta taagacagaa agaacgttac
      481 aaacatgaag attaacatat gaaatc
//
LOCUS       DAZKFZ010000589          505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000589 DAZKFZ010000000
VERSION     DAZKFZ010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.593"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>505)
                     /locus_tag="ACHOQW_005265"
     CDS             complement(<1..>505)
                     /locus_tag="ACHOQW_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576111.1"
                     /translation="KHSILSSLQDKEDDVDELKYSAEDFDSLTVADLYDIEIAMQDFL
                     NDINFENSKDNKVRFDEDTYDFNINGKRRGMFGKGTRAVMHAIFTICFAEFLSRKGNP
                     FIGFVVLDSPLVTHFDKDRGGSLSDVNSVSLSDSFYHALIKRDYNFQIVILENKGPTF
                     QIKINDA"
BASE COUNT          158 a           99 c           75 g          173 t
ORIGIN      
        1 tttgcatcat taatttttat ttggaacgtc ggacctttat tctcaagaat tacaatctgg
       61 aaattatagt cccttttgat taatgcgtga taaaatgaat cactgagact gacgctatta
      121 acatccgata gagaaccccc tctatcttta tcaaagtgcg tcactaatgg actatcaagc
      181 acaacaaatc caatgaatgg attccctttt cttgataaaa attccgcaaa gcaaatggta
      241 aatatagcat gcattactgc acgggtacct ttaccaaaca ttcctcggcg tttcccattg
      301 atattaaagt catatgtatc ttcgtcaaat cgcactttgt tatctttaga attttcaaaa
      361 tttatatcat tcaaaaagtc ttgcatcgct atttctatat catacaaatc agctactgtt
      421 aaggagtcaa agtcctcggc ggagtattta agttcgtcaa catcatcttc tttatcttgt
      481 aatgaagata gaatgctatg ttttg
//
LOCUS       DAZKFZ010000590          504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000590 DAZKFZ010000000
VERSION     DAZKFZ010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.594"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..302
                     /locus_tag="ACHOQW_005266"
     CDS             <1..302
                     /locus_tag="ACHOQW_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9576112.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHOQW_005267"
     CDS             299..>504
                     /locus_tag="ACHOQW_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9576113.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALW"
BASE COUNT          114 a          116 c          170 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcaggggc
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct accgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZKFZ010000591          504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000591 DAZKFZ010000000
VERSION     DAZKFZ010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.595"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>504
                     /gene="escU"
                     /locus_tag="ACHOQW_005268"
     CDS             <1..>504
                     /gene="escU"
                     /locus_tag="ACHOQW_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFP9576114.1"
                     /translation="NFTGLNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEG
                     LKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIA
                     ICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARTLYKNIHKGQYI
                     TEDFFEPV"
BASE COUNT          167 a           88 c           99 g          150 t
ORIGIN      
        1 taatttcaca ggactgaacg catatcaagc tcttgtcgtt gttgcctttt ttgtttttct
       61 tttatggaaa ggcgtgctat tcggatatct actcttttca gtatttgatt tctggttcca
      121 gaagcatgag ggactgaaga aaatgaaaat gagtaaagat gaggtgaaac gagaagccaa
      181 ggatactgat ggtaaccctg aaattaaagg ggagcgccgt cgccttcatt ccgagataca
      241 aagtggaagt ttggctaata acatcaaaaa atcaaccgtt attgttaaaa acccgactca
      301 cattgcgatt tgcctatact ataaacttgg ggagactcca ttacctttag ttattgaaac
      361 aggaaaagat gccaaagctc tacagatcat taaactggct gaactctatg atattccagt
      421 gattgaagat attcctttag caagaactct ctataagaat atacataaag gacaatatat
      481 aacagaagac ttttttgaac ctgt
//
LOCUS       DAZKFZ010000592          504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000592 DAZKFZ010000000
VERSION     DAZKFZ010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.596"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(75..422)
                     /gene="tnpB"
                     /locus_tag="ACHOQW_005269"
     CDS             complement(75..422)
                     /gene="tnpB"
                     /locus_tag="ACHOQW_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9576115.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          112 a          141 c          140 g          111 t
ORIGIN      
        1 tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt ctgtgacaac
       61 caggataaag agatttacag catggtcagg gaggtcagca gccgcttagg ctgtcgccag
      121 tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt gccatcacgg
      181 gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca cagtccgtca
      241 ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat gaaaacatgg
      301 ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc gttgaagcca
      361 tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg taacgggatc
      421 atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg ccattgaagc
      481 ggagcgtccc gtgccggaac gtta
//
LOCUS       DAZKFZ010000593          503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000593 DAZKFZ010000000
VERSION     DAZKFZ010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.597"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>503)
                     /gene="espY3"
                     /locus_tag="ACHOQW_005270"
     CDS             complement(<1..>503)
                     /gene="espY3"
                     /locus_tag="ACHOQW_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFP9576116.1"
                     /translation="SGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNANCTN
                     ANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKSDGNE
                     TKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYRTDLF
                     NPILKTE"
BASE COUNT          137 a           95 c           86 g          185 t
ORIGIN      
        1 cttctgtttt taatatcgga ttaaaaaggt cggtacggta aagtttcgtg cctttcatcc
       61 tgacgatgtc aggaagaata gtgtttgaaa gattagcctc gctaaagtcc acattttggc
      121 aatcactttc tgataaatct gcccaattga aagtggtttt cacacaggag cagttagtta
      181 gaatcatccc tttcgtttca ttcccatctg atttctgata agttatcctt tgtattattt
      241 gatctgcttc tggatattgc atctcaggtt ctttttcttt accatatatc atcgcattat
      301 caagaattgc gccagtgcaa ttcatatttg tcaggtcgca tttcttaaat ctacaattgt
      361 taaaattcgc atttgtacaa ttcgcatttg taaacttgac gccataaaaa taagtatgtt
      421 cgaagttgac atttgagcag ttacagtttc ccatatctat accgaaatct atatcgggat
      481 ctttattggg atctttaccg gaa
//
LOCUS       DAZKFZ010000594          503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000594 DAZKFZ010000000
VERSION     DAZKFZ010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.598"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            125..496
                     /gene="grlR"
                     /locus_tag="ACHOQW_005271"
     CDS             125..496
                     /gene="grlR"
                     /locus_tag="ACHOQW_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFP9576117.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVLSNDNNLTLHCHVRGNEKLFVDV
                     YAKFIEPLVIKNTGMPQVYLK"
BASE COUNT          179 a           64 c           98 g          162 t
ORIGIN      
        1 tgagtatatt taatgatata ttacattgca atctggagaa aaagaaaggt ctccattatt
       61 cttgatattg cttatggata gaacaaattg aaaggagtga ggttagtatg aaactgagtg
      121 agttatgatt atgaaggatg gcatctatag cattatattt attagcaatg aagactcctg
      181 tggggaaggt atactgatta aaaatggaaa tatgatcact ggcggcgata ttgcttctgt
      241 gtatcagggg gtcctctctg aagatgagga catcatactt catgtccatc gatataatta
      301 cgaaattccc tcggtgctaa acattgaaca agattatcaa ttagttatcc ctaaaaaagt
      361 actgagtaat gataataatc tcacattaca ttgccatgta agaggaaatg aaaaattgtt
      421 tgttgatgtt tatgccaaat ttatagaacc attagttatt aaaaacacag gaatgccaca
      481 agtttattta aaataatttt atg
//
LOCUS       DAZKFZ010000595          502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000595 DAZKFZ010000000
VERSION     DAZKFZ010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.599"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            28..>502
                     /locus_tag="ACHOQW_005272"
     CDS             28..>502
                     /locus_tag="ACHOQW_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFP9576118.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVII"
BASE COUNT          203 a           94 c           87 g          118 t
ORIGIN      
        1 ccgttggatt ggtatataat aaaagccatg ctctggactg aaaacgatgc ggaaaatact
       61 tctcagtgga atggttatcc attacaaatt ggtcgttttc ggaaagataa agcgatgcca
      121 gctttaatct ctggagaaaa atctacagcc cttgttaccc caccacaatg gagaaacaaa
      181 gcattcaatg ggttaaaaga tccagaaaga aattactggg caaaagaaca aataactggc
      241 tctccagaag agaatatcaa agcagcaatt acttacttaa tgatgaaact ttctaataca
      301 aaggaagaat caactatcga tcaatatgac tcaactctat atagcgccat tgtccaaaaa
      361 ggcgatttgg ctgacaacat cagaaaagag agaaaaacga ccatacctaa cttaacgaaa
      421 aacaatcctg gaaaaaatct cgataaaata cacccgggag atattttata ttatcaaaaa
      481 gcatccatga aagtaataat ta
//
LOCUS       DAZKFZ010000596          498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000596 DAZKFZ010000000
VERSION     DAZKFZ010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.600"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>498)
                     /locus_tag="ACHOQW_005273"
     CDS             complement(<1..>498)
                     /locus_tag="ACHOQW_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9576119.1"
                     /translation="IYSGFKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGE
                     TARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSR
                     KAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDE
                     VMLKN"
BASE COUNT          146 a          106 c           78 g          168 t
ORIGIN      
        1 tattttttag cataacctca tcataacatg agtttttctt gcataatgta ttatctgaat
       61 ttgatggctc aatattaatt gtagggattc gcttacatac acctttacat cccccgtcat
      121 tttcaatcca gagtaaattt acccccgttc tgtacagaat atccagtaca ttttccgaat
      181 ttaccgcttt gcgggaattg aactcttttc gcttcatatc tgaaaacatg catggaacag
      241 atatggctgt ggatgtgcca cacgactgca cattatgaaa ggatataagt tcattatatt
      301 tctttgtaaa atcattggta ccacgagaat atccattcag agcatagttt tgacttcttg
      361 ctgtctcccc gacaactaaa aacataatta ccggcttttg tttttcattt attgtgcggg
      421 atgcatccgt tccagtctga cgaaactcgc caggagaaac aaaaaaatcc cttacatatt
      481 taaacccact gtagatat
//
LOCUS       DAZKFZ010000597          489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000597 DAZKFZ010000000
VERSION     DAZKFZ010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.601"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..465)
                     /gene="nleG"
                     /locus_tag="ACHOQW_005274"
     CDS             complement(<1..465)
                     /gene="nleG"
                     /locus_tag="ACHOQW_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFP9576120.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNS"
BASE COUNT          135 a          108 c           98 g          148 t
ORIGIN      
        1 tgagtttttt gcatttctga caaaaacacc agtttcagga atgcataatg ttatcgggca
       61 attcaggtgt tgttcatgac aggaaaagtc ttgatgacta acagtaaatg catgagagtt
      121 aattttgttt tgtattgcac tactgacgtt ttggcgcgta catggggttg cacggctctg
      181 ttccatatat aaacggaatg cctgcaaaaa agtatggcct ctgttaagct gtatctccag
      241 attttctgcg agaaggcgat gccctcttgc actaaggcga tccaacagtc cgccggaaac
      301 cggttccacg ctaaaactgt ccataaatga aatatgatga agtctcatag tgcggcctct
      361 cacaacaagc tgttcactct gactaactct tccgacatga cgtaaagcct ccagttcact
      421 ttcagacaat actctttcac tcgaaaagtt taatatgaca ggcatatttt ctccctcttt
      481 agtctatgt
//
LOCUS       DAZKFZ010000598          487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000598 DAZKFZ010000000
VERSION     DAZKFZ010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.602"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..197)
                     /locus_tag="ACHOQW_005275"
     CDS             complement(<1..197)
                     /locus_tag="ACHOQW_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9576121.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHR"
     gene            239..466
                     /locus_tag="ACHOQW_005276"
     CDS             239..466
                     /locus_tag="ACHOQW_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9576122.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT          147 a          129 c          104 g          107 t
ORIGIN      
        1 cggtgatcga gttcactgtc acaaaggtca accacacgcc cacagagaca gcactgtccg
       61 gcatccctga gccagatacg acgcttgagg gaaacccggg cactgccact gacacgacgc
      121 tgttcaccct tcaggacatt cacccgccgg gtgttcagag ttttgattct gcccggtaac
      181 gtacgaagca cagccatgta aaatcctcgc catataactt gtcaccagag gaaagaaaat
      241 gtcatcgaaa aaccggaccc gcagaacaac aacccgcaac atccgatttc caaaccagat
      301 gattgaacaa attaacatcg ctcttgacca gaaaggttca gaaaattttt ctgcgtgggt
      361 cattgaatct tgccgccggg agctggcagc agacataaaa tatgcccgtc agttgactat
      421 aaaaaagaat gatacacagt atgctctgcg atggctgttc atataactat ttctttatat
      481 tgctgaa
//
LOCUS       DAZKFZ010000599          484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000599 DAZKFZ010000000
VERSION     DAZKFZ010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.603"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..397
                     /gene="espX1"
                     /locus_tag="ACHOQW_005277"
     CDS             <1..397
                     /gene="espX1"
                     /locus_tag="ACHOQW_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFP9576123.1"
                     /translation="TIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCEDIMPGRWTTQ
                     LVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHD
                     YDKTSPEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          196 a           89 c           73 g          126 t
ORIGIN      
        1 caccatcttt tcaaatggag aaataaacaa cctcttcatt aaaaaaagca acatatttgg
       61 aacatcgttc actaatacca tgataaaaaa tttgcgctgt gaagatatta tgcctggccg
      121 atggaccact caacttgtca ataagcactt aggttacaga tacacaggag tattcaaaac
      181 cctggcaagt attgatgaca aaccgtcccg tttcgaaatt ttgataccat tggtacaaac
      241 tttagttcgc gacaatgtaa agctaaataa tgatgtgtat aaagaactga aaaagtttat
      301 gcacgattac gacaaaacat ccccagaaat gagaaaatac cttcagagca tcaacgagtc
      361 tatgttcctt atgaaaaaaa tatctcatca agattaaaac accccaaaaa agaagtaatt
      421 tcagaatgaa attacctgtg ataattatta attaactgca gagaaataaa aaataatctg
      481 aagc
//
LOCUS       DAZKFZ010000600          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000600 DAZKFZ010000000
VERSION     DAZKFZ010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.604"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(165..239)
                     /locus_tag="ACHOQW_005278"
                     /pseudo
     CDS             complement(165..239)
                     /locus_tag="ACHOQW_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          153 a           91 c           96 g          143 t
ORIGIN      
        1 aataaataaa cggatcaagt cgttcctatg acaatgaggt tatacgaaaa gacgcttact
       61 catcagaaat ctggtatgct ttaattaaac cagtgtcgct gatgtgtaag cgttgacgtt
      121 gggagacgat taaatggtta taccactcct acgtcttttc catacggcgt ataagagtgc
      181 aacatgttga gttttccgag tgaatggtgc attttactaa ccgagccatt tgaatcaacc
      241 aacgcaataa aaaagactgg aataataagc ctgtatttat tatttatcgc aatcgcactt
      301 tacggtttta tccaactgaa aagagactgc ttccagcggt tgtggattat ccctgggcga
      361 tagtcatagt ctggtgtcag gattcacaaa tgacctgttt ccttacatgg atataaacac
      421 aacatagagt gcggtagtta atctgaataa tctaatttcg gctattcagc acaaaacaac
      481 att
//
LOCUS       DAZKFZ010000601          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000601 DAZKFZ010000000
VERSION     DAZKFZ010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.605"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..366
                     /locus_tag="ACHOQW_005279"
     CDS             <1..366
                     /locus_tag="ACHOQW_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9576124.1"
                     /translation="DRMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDI
                     SGLAFNNESLMILSDESRKLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPD
                     ESIYVASEPDILAKFVPNK"
BASE COUNT          160 a           95 c           87 g          141 t
ORIGIN      
        1 gatcggatgc tgtttgtcgc aaaagaaaga cgcccaagta aaatatttat atatcaactt
       61 tctccagacc tgctgagtgt aaagcaagca actattccag aggcactaaa tgatatacgt
      121 gttaacgata tatccggcct ggcctttaat aatgaaagtc tgatgattct gtccgatgaa
      181 tcgcgtaaat tactcaagtt taacctgaca gagatgtctt tcattgagat gcttgacctg
      241 acaaaaggaa atcattctct gaccagtgat ttgccccaac ctgaaggaat tgtaactctg
      301 cctgatgaaa gcatttatgt tgccagtgaa cctgatatcc tcgcgaaatt tgtccctaat
      361 aagtaagtct gtcactggcc ttattattta tcgaccaaac ctggcaatct cataaagtta
      421 taaaaatacg agtataattt cacgctaaat aaacacatta atagttgaaa aggatatcag
      481 tta
//
LOCUS       DAZKFZ010000602          482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000602 DAZKFZ010000000
VERSION     DAZKFZ010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.606"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>482)
                     /gene="escQ"
                     /locus_tag="ACHOQW_005280"
     CDS             complement(<1..>482)
                     /gene="escQ"
                     /locus_tag="ACHOQW_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFP9576125.1"
                     /translation="ENRLNVIILETTVQSLQYLINDNWQLVPHSNALFFDGYIVPGWT
                     DYPVTELRVGDSLRLYHVDDSQERRCWIVINTPLATVNLSDNNLSVTDVLAADLLSAL
                     SNETVMNRIYCAIGTVHIDIHMLRNVKKDDIINSDGYHLFGGCQLIRNNTTIAYGSIV
                     "
BASE COUNT          162 a          102 c           72 g          146 t
ORIGIN      
        1 tacaatactc ccgtatgcta tcgtcgtatt attgcgtatt aactgacaac caccaaaaag
       61 atggtatcca tctgaattaa taatgtcatc ttttttaaca ttacgcaaca tgtgtatatc
      121 aatatggact gtaccgatcg cacaataaat gcgattcatg acagtctcat tgctaagagc
      181 agataacaga tccgcagcca gaacatccgt gacagataaa ttattatcac tcagatttac
      241 tgttgctaaa ggggtattaa taacaatcca gcaccttctc tcttgactat catcaacatg
      301 atagagtctc agactatcac caactctcaa ctctgtaact ggataatcag tccacccagg
      361 tacgatatag ccatcaaaaa aaagagcatt agaatgtgga actaattgcc aattatcatt
      421 aatcagatat tgtaaacttt gtaccgttgt ttcgagtata attacattaa gtctgttttc
      481 tc
//
LOCUS       DAZKFZ010000603          472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000603 DAZKFZ010000000
VERSION     DAZKFZ010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.607"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(46..375)
                     /locus_tag="ACHOQW_005281"
     CDS             complement(46..375)
                     /locus_tag="ACHOQW_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9576126.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(372..>472)
                     /locus_tag="ACHOQW_005282"
     CDS             complement(372..>472)
                     /locus_tag="ACHOQW_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9576127.1"
                     /translation="LMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT           98 a          173 c           98 g          103 t
ORIGIN      
        1 ctgatttaac cgactcgttc agactttctt cgtgaatatc ctgcatcagt tcaccacctg
       61 cttaaactcc gcgctgaact caacgcgcaa catcccgacc cgcgcagacc acccggcaca
      121 ggtcaccttt atctgccggt atgcataggg tggcttccac aaaaatgcct tccagcctcc
      181 gtgctctgcc aggaacgctt ccagatgccg ggcctcctcc cgggtcacgg aaagcgtcac
      241 acggtatgtt ttcaggtcag cattcagccc tgccgccata cgctgtgagt acccgtcacc
      301 aaaacgcact tcacgcaccg atggctgcga gttcacctcc atatccggct tcactttcca
      361 gcgaaaggtt ttcatcgccc gctccccgat aacataccgc catcacgcaa ctgcagccgg
      421 agctcatcct gcgccccctt gcgggccatg tcatacaccg ccttcatcag ct
//
LOCUS       DAZKFZ010000604          472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000604 DAZKFZ010000000
VERSION     DAZKFZ010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.608"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>472
                     /locus_tag="ACHOQW_005283"
     CDS             <1..>472
                     /locus_tag="ACHOQW_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP9576128.1"
                     /translation="HVLVNGINNDSLAQMQYWTAVRNIIDDTNEVTIDLSYNLAITNI
                     DTSDEHLVEVSDNSEGNYIKDNDSMSIRYRSKYYSRDSALIEEETIFSDAELKAILPM
                     RRMYGVSDYKSNSSSLSSHSGLKDPTGTPVCYYIHNESKPSLGYGPTSNNWLSQS"
BASE COUNT          162 a           92 c           82 g          136 t
ORIGIN      
        1 ggcatgtatt agttaacggc atcaacaatg attcacttgc acaaatgcag tactggactg
       61 cagtaagaaa tataattgat gacactaatg aagtgaccat tgatttatct tataatctgg
      121 caatcacaaa tatcgatacc agtgatgaac atcttgtaga agtaagcgat aattcagaag
      181 gtaattatat aaaagacaat gactcaatgt ctattcgtta tagatcaaaa tattattcca
      241 gagattccgc cttaatagaa gaagaaacaa tattttctga cgcagaacta aaagctattc
      301 tgcctatgcg tcgcatgtac ggggttagtg actataagtc aaattcctct tctctatcct
      361 cacactcggg actcaaggac ccaacgggca cacccgtctg ttattatatt cataatgaga
      421 gtaaaccttc cttaggttat ggtccaacat ccaataattg gttaagccaa tc
//
LOCUS       DAZKFZ010000605          470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000605 DAZKFZ010000000
VERSION     DAZKFZ010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.609"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..454
                     /locus_tag="ACHOQW_005284"
     CDS             <1..454
                     /locus_tag="ACHOQW_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9576129.1"
                     /translation="IEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSRHSE
                     LAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGA
                     LLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          118 a          118 c          141 g           93 t
ORIGIN      
        1 catagaggcg gatataaggg gaatgccggc agagcagcgg cttgctgaac gtcagcgaaa
       61 aacgaaaccg ttgttgaaat ccctggaaag ctggttgcgt gaaaagatga agaccctgtc
      121 gcgacactca gagttggcga aggcgttcgc gtacgcactt aaccagtggc cggcactgac
      181 gtactatgcg aacgatggct gggtggaaat cgacaacaac atcgctgaaa atgccctgcg
      241 ggcggtcagt ctgggtcgta aaaacttcct gttcttcggc tctgatcatg gtggtgagcg
      301 gggagcgcta ctgtacagcc tgatcgggac gtgcaaactg aatgacgtgg atccagaaag
      361 ctaccttcgc catgtgcttg gcgtcatagc agactggccg gtcaaccggg tcagcgaact
      421 gcttccgtgg cgcatagcac tgccagctga ataacacatc cccgtcaata
//
LOCUS       DAZKFZ010000606          469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000606 DAZKFZ010000000
VERSION     DAZKFZ010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.610"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>469
                     /locus_tag="ACHOQW_005285"
     CDS             <1..>469
                     /locus_tag="ACHOQW_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFP9576130.1"
                     /translation="QRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLVEHV
                     RGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMILDNF
                     KLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNIL"
BASE COUNT          134 a           86 c          106 g          143 t
ORIGIN      
        1 tgcagcgcat ttcagaggta attcagcgtc tgatccagga gcgaatttca gtgcgcaata
       61 tgcggttagt tatggaggcc ttagcattat ggagcccacg agagaaagat ataatcacac
      121 tcgtcgagca tgtacgaggt gcattaggtc gctacatttg ccataagttt tcatattctg
      181 gagaaattaa agcaattgtg atttcgcctg aaatagagga cagaatcaga gatggtgtca
      241 ggcctaccgc tggcggtact tttcttaatc tcgatgcatc cgaagctgag atgattctgg
      301 ataattttaa actggcactt tctggaatca atattcctat taaagacatt attttactgg
      361 gatctgtgga tattcgtcgt tttatcaaaa aacttattga atctagttat cgagacctcg
      421 aagtactttc gtacggggaa ctaacggaaa atgttcccgt taatattct
//
LOCUS       DAZKFZ010000607          466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000607 DAZKFZ010000000
VERSION     DAZKFZ010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.611"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(88..>466)
                     /locus_tag="ACHOQW_005286"
     CDS             complement(88..>466)
                     /locus_tag="ACHOQW_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576131.1"
                     /translation="ACLSNPQGITCFYYSPLQGYQSFLKNTSQDVSELLSKSLNGTIS
                     STSGLKKSGNAFFGTGKYLKEIDFTINGFSQKMYLMAVNNVGNEVKLQCSCYFTTGSV
                     DEEKSKSDKIDFTFSGAATTADL"
BASE COUNT          160 a           94 c           71 g          141 t
ORIGIN      
        1 gctcattttt tcaaaaagac tgtttacaca cagacggtta acaatacgtg ttcaccttaa
       61 tgataaacgc gctttatcaa gaaaaaatca taaatcagca gttgttgcgg caccggaaaa
      121 tgtaaaatcg attttatcgc ttttggattt ttcttcgtcg acactaccgg tagtaaaata
      181 acaagagcac tgaagcttca cttcatttcc aacattatta accgccatca aatacatctt
      241 ctgactaaac ccatttatag taaaatcaat ttctttaaga tacttcccag tcccaaaaaa
      301 ggcatttcct gactttttta aacccgatgt tgatgaaatg gtaccattca aactctttga
      361 gagtaactcg ctaacatctt gagatgtatt ttttaaaaaa ctctgatagc cttgcaaagg
      421 agaataataa aaacatgtaa tcccctgagg gttggataag caagcg
//
LOCUS       DAZKFZ010000608          464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000608 DAZKFZ010000000
VERSION     DAZKFZ010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.612"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            9..>464
                     /gene="ehxC"
                     /locus_tag="ACHOQW_005287"
                     /gene_synonym="hlyC"
     CDS             9..>464
                     /gene="ehxC"
                     /locus_tag="ACHOQW_005287"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP9576132.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHE"
BASE COUNT          158 a           65 c          103 g          138 t
ORIGIN      
        1 taaacattat gaaatctaat gcttttgacg tcatggggaa ggttgcatgg ctatgggcct
       61 gttctcctct gcataaaaaa tggccattat ctgtttttgc tattaatgtc atacctgcta
      121 ttcagacaaa tcaatttgct ctcttaataa aggatgagct tcctgtagct ttttgtagct
      181 gggccagttt agatctggaa tgtgaggtaa aatatataaa tgatgtaaca tctttgtatg
      241 caaaagactg gatgtcggga gaaagaaagt ggtttattga ctggatagct ccatttggac
      301 acaacatgga gttatataaa tacatgagga aaaagtatcc ttatgagtta ttcagagcaa
      361 taaggttaga tgagagcagt aaaacaggaa aaatagctga atttcatgga ggggggatag
      421 ataaaaaact ggcatcaaaa atattcaggc aataccatca tgaa
//
LOCUS       DAZKFZ010000609          464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000609 DAZKFZ010000000
VERSION     DAZKFZ010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.613"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            182..>464
                     /locus_tag="ACHOQW_005288"
     CDS             182..>464
                     /locus_tag="ACHOQW_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFP9576133.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPR"
BASE COUNT          144 a           80 c           97 g          143 t
ORIGIN      
        1 ttcagcttat tgatagaaag attgattgcc agtctgttta gagccgtaca gcagcaaact
       61 ttcataacca ggttgatcct gaatttgcag tacgaaaagt taaaaatgag cgatttctgt
      121 aagactcccc attttgccag gcttatgatt cgcagatcat tcaagtgcaa aggagcgatg
      181 tatggattgt tcaaaatgca atggttatgc gacgatgtta ttaaatatgg tgcagggttc
      241 tgacccggtt aatttactgg aattacatgg tttccttgaa catttcgcat attatgtttc
      301 atttggcaaa tttaatgctg gccaccagcg ctataatgca tttaaaaaat tcgtcagtga
      361 gatttcggag atcagcgcaa atgatatcaa tatgacgatt aaaaccgggc aatccagaca
      421 tgaaaatgtg atctctataa atatgaatga tgcaattcct cgtg
//
LOCUS       DAZKFZ010000610          462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000610 DAZKFZ010000000
VERSION     DAZKFZ010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.614"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..228
                     /locus_tag="ACHOQW_005289"
     CDS             <1..228
                     /locus_tag="ACHOQW_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9576134.1"
                     /translation="PLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIG
                     PEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            221..>462
                     /locus_tag="ACHOQW_005290"
     CDS             221..>462
                     /locus_tag="ACHOQW_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9576135.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPP"
BASE COUNT           85 a          112 c          169 g           96 t
ORIGIN      
        1 cccctgaccc gggcggcgga tggctcgctg ggcgtacgcg ccgtgggcag tatgaacggc
       61 agtgctggtc tggtgtattc cccggtctac cacatcgcca ttcagaatga cggggctaac
      121 ggacagatag ggccggaggc ggcaggcagt cttgtgcagc tgattgacca gcgggtgcag
      181 gcggtgatgc tgtccatgcg acgtgacgga ggaatgctga gtggctgaga taaaaacgct
      241 gcatctggtc ccgcgtgaag ggatgcaggt gagtgagaag ccgtcggtgg tgagggtgcg
      301 gtttggtgac ggttatgaac agcgccgccc cacagggctg aatcctcaac tgaagacgtt
      361 tcaggcggtg ttccgggtga cggatgagtc aacccggcgc tggctggatg aatttttatc
      421 ctggcatggt ggttaccgtg cctttttgtg gcgaccgccg aa
//
LOCUS       DAZKFZ010000611          461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000611 DAZKFZ010000000
VERSION     DAZKFZ010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.615"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..137)
                     /locus_tag="ACHOQW_005291"
     CDS             complement(<1..137)
                     /locus_tag="ACHOQW_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576136.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     K"
BASE COUNT          135 a          105 c           92 g          129 t
ORIGIN      
        1 atttttaacg ttttatactt cacattacaa tctgtaccat aagcctcctc agcgaggttg
       61 cctgaatacc ctgtcgtgcc catttttcca tcggacgcag ttgtctgctc tctcgaacta
      121 ctggcaataa aaatcaacca gacagcttga tgtccgttac atgcaggact aatcacgatt
      181 ggttaccgtt tacaaaactt gcgtaacatt ggaggagaac gaataatatt ctctagctgg
      241 tacgcgttcc atagcgaaac gggcaaggtc caacagcaag agctgaaact cagggggatt
      301 tatcagtcga tttattcgcc atggatgaat cacgctgtac ggttatggta gctcttagcg
      361 acaaggcata atcatcagta caccaatgta acaaccgttg aaaaagactc attattttat
      421 gaaccagcca tcccatttcc agagtggtaa atatgatttg t
//
LOCUS       DAZKFZ010000612          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000612 DAZKFZ010000000
VERSION     DAZKFZ010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.616"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(246..>460)
                     /locus_tag="ACHOQW_005292"
     CDS             complement(246..>460)
                     /locus_tag="ACHOQW_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP9576137.1"
                     /translation="IADKSSTKNCILDSTTCNVDGFTVFNRRSCSFDMRPPGSADRTP
                     QLRLSISEVAWMSKIIETETNNTNKS"
BASE COUNT          135 a           83 c           89 g          153 t
ORIGIN      
        1 ttttcgtgtc tttaagtgat accagatggt attgcgctaa atgtaccaat gggtatacca
       61 agagcactaa aaacaaaggg gcaaccactt tgtaacccct tgattatttg cagaagcgca
      121 gaaaatcgaa ctctggaacc ctttcggggc atcctttttc aagaatgaag aaaaaaataa
      181 ctaaaaaatc aagttgttag gaagttattc atggctagtg taccaactct tttctgggct
      241 aatagctatg atttgtttgt attatttgtc tctgtctcta ttatttttga catccatgcg
      301 acttctgata ttgataacct cagttgtgga gttctgtctg cgcttccggg aggacgcata
      361 tcaaaactac aggatcttct gttaaacact gtaaagccat ctacattgca tgttgttgag
      421 tctaagatac aatttttagt agatgattta tctgctatag
//
LOCUS       DAZKFZ010000613          459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000613 DAZKFZ010000000
VERSION     DAZKFZ010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.617"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(112..303)
                     /gene="yfgG"
                     /locus_tag="ACHOQW_005293"
     CDS             complement(112..303)
                     /gene="yfgG"
                     /locus_tag="ACHOQW_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP9576138.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            321..>459
                     /locus_tag="ACHOQW_005294"
     CDS             321..>459
                     /locus_tag="ACHOQW_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576139.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKSSVDTYFNVMEVNLIIYND
                     DV"
BASE COUNT          141 a           80 c          113 g          125 t
ORIGIN      
        1 aagacgtata atttaagatg aaagaatatc tgccgctttg ataattccaa agagcaaaag
       61 catccgacag cgaagcgccg ttatgttctg tgaaagtgga gaaggtaacc gctaacgttg
      121 taccggtgat tcgacggaga gtgtggattg ctgagcttct tttttgctct gatggtgctg
      181 ccaggcaccg acggacgagt agataaaacg gccaaagaag aagataaagc tgatgagcag
      241 tacgatacgg gtcatgcgac tgttaaatcg gtgtcgtttt cgcatactgg ttgcctgact
      301 cacaaaaggt tccttgaagt atgtcccacg ccgtggacgg tacttacatt aaggcacaac
      361 aggacaaaat ggtcaattct tcgttatgta aaaaatcgtc agtggataca tattttaatg
      421 ttatggaagt taatttaatt atttacaatg atgatgtaa
//
LOCUS       DAZKFZ010000614          450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000614 DAZKFZ010000000
VERSION     DAZKFZ010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.618"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          129 a           94 c           81 g          146 t
ORIGIN      
        1 aatttttgct ttatactgta gatttcacgt atgtaagtaa tatatgaaaa ggctctcgtt
       61 aagatgtctg gtacaaaatg acagatcata tgccttttgt aaagagacga cagaagttga
      121 gtcttacagg cacatcataa ataccctccg gccatgccgg agggtattta tttaactgcc
      181 gctgtttgca ctggatgtgg aactactcat actataagaa ggtccacttc cccccggata
      241 catgatgatg gatggagtac ccggagccca gcatgctcca ttagcatcaa tatatgttcc
      301 catatctttt cctcgtagat ctattacatt agtagaacta ggtaatccat taagattcat
      361 ccctcctttc acgaaatcaa gcatgtgatc tgatatttct ctcattttaa aaacctcctg
      421 ctcatttatg ttcagatatt aatttggtgt
//
LOCUS       DAZKFZ010000615          445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000615 DAZKFZ010000000
VERSION     DAZKFZ010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.619"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(254..>445)
                     /locus_tag="ACHOQW_005295"
     CDS             complement(254..>445)
                     /locus_tag="ACHOQW_005295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576140.1"
                     /translation="ILPPYSKNTQAFRTLYQPHLDCFPFPDTAARGVIKVRNFQQYGV
                     LLFTAVMHQPVLAIVVITV"
BASE COUNT          129 a           92 c          103 g          121 t
ORIGIN      
        1 aggaatacat tcaaaaaatt tcttttttca tttcaggagc gattttcatc tgttacttga
       61 agtatctgtg atattaagtg actcccccta acgtcgtact aaatagtaat tactggccat
      121 accgggagca gtattcggcg ctccccacat gcagcagaat aaccgcatca gcgaagatgt
      181 atttttacta tcacaatgtg tacggacggt ttatggaaaa gaatgaacat tagatacgcg
      241 atgcggcggc aggttacacg gttatcacta cgatagccaa tactggctgg tgcattaccg
      301 ccgtgaacaa taggacgccg tactgctgga aatttcttac ctttataact ccccgcgccg
      361 ccgtatcggg aaacgggaag caatcaagat gaggctgata taatgtgcgg aatgcctgag
      421 tatttttaga ataggggggt aatat
//
LOCUS       DAZKFZ010000616          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000616 DAZKFZ010000000
VERSION     DAZKFZ010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.620"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            25..417
                     /gene="ydeI"
                     /locus_tag="ACHOQW_005296"
     CDS             25..417
                     /gene="ydeI"
                     /locus_tag="ACHOQW_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP9576141.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          129 a           91 c          110 g          113 t
ORIGIN      
        1 accaaacgca attaaggagt tagtatgaaa tttcaggcca ttgtattagc aagttttctt
       61 gtcatacctt atgcgttggc agatgaccag ggcggtttaa aacaggatgc agcgccaccg
      121 ccgcctcatg caatagaaga tggctatcgc ggaaccgatg atgcaaaaaa aatgactgtt
      181 gatttcgcaa aaaccatgca cgatggcgct tcggtttcac tacgcggtaa tttgatttct
      241 cacaaaggag aggaccgtta cgtttttcgc gataagagtg gtgaaattaa tgttgttatt
      301 cctgcgaccg tttttgatgg acgagaagtt cagccggacc agatgatcaa cattagcggc
      361 agtctggata agaaaagcgc gccggccgtc gttcgggtca ctcatttgca gaaataaaaa
      421 taaattagcc tgatggcctg aac
//
LOCUS       DAZKFZ010000617          442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000617 DAZKFZ010000000
VERSION     DAZKFZ010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.621"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..166)
                     /locus_tag="ACHOQW_005297"
     CDS             complement(<1..166)
                     /locus_tag="ACHOQW_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9576142.1"
                     /translation="MNILKKLMQRLCGHGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQ"
     gene            complement(168..>442)
                     /locus_tag="ACHOQW_005298"
     CDS             complement(168..>442)
                     /locus_tag="ACHOQW_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9576143.1"
                     /translation="ATGPQGPKGDPGETQIRFRLGPASIIETNSHGWFPGTDGALITG
                     LTFLAPKDTTRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT           87 a          143 c          100 g          112 t
ORIGIN      
        1 cctgcgtgat taccctgtcc tgctccggaa taataccatt ctcatcggac tccaggatgt
       61 ctgccggccc cagacgcagt tgtgctgtaa gtaactcccc gtcttcacgg tcatcatgct
      121 ttccgtgccc gcacagacgc tgcataagtt tttttagtat gttcatgtca ttctcctgtt
      181 ctgcctgtat cactgcccac ttcatcaagc cccttaacat cctgccacgg cccgtcacca
      241 aacctgacct gcaaatgctg aaaaaaaccc tgaacccgtg tggtatcttt gggggcaaga
      301 aaggtcagtc cggtgatgag cgcaccatct gtacccggga accagccatg gctgtttgtc
      361 tcaataatgc tcgccggccc cagacgaaaa cggatttgtg tctcccccgg gtcgcccttc
      421 ggtccctgag gtccggttgc cc
//
LOCUS       DAZKFZ010000618          440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000618 DAZKFZ010000000
VERSION     DAZKFZ010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.622"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(47..151)
                     /locus_tag="ACHOQW_005299"
     CDS             complement(47..151)
                     /locus_tag="ACHOQW_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576144.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(267..>440)
                     /locus_tag="ACHOQW_005300"
     CDS             complement(267..>440)
                     /locus_tag="ACHOQW_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9576145.1"
                     /translation="DGQHVIILCDGAFVLYAQYRDGEFFDVVRNGDEFFETQSRNVTD
                     WMPLPEPPQEVRQ"
BASE COUNT          136 a          123 c           77 g          104 t
ORIGIN      
        1 gcaaaccgtt tttacccgat atgggaattc ccatatcgta atgaattcag ttccctagtc
       61 gatccatcaa aaacacaacc aggcagtaaa cgcccacaac agcaataaca gccagcgcac
      121 cttccattgc cagtgaaata tcatccgaca tattccctcc tttggtgtga atcccggcga
      181 acgtttttac ccccaccgac aaataacata tactaaaaaa tcaatagcta tagcaacgcc
      241 tgtaattgca aaggcttcag gccagatcat tggcgcacct cctgcggcgg ttctggtagc
      301 ggcatccagt cggttacatt gcggctctgt gtttcgaaaa attcatcacc attacggact
      361 acatcaaaaa actcaccgtc tcgatattgc gcataaagaa cgaatgcgcc atcacataaa
      421 ataattacgt gctgaccatc
//
LOCUS       DAZKFZ010000619          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000619 DAZKFZ010000000
VERSION     DAZKFZ010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.623"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..75)
                     /locus_tag="ACHOQW_005301"
     CDS             complement(<1..75)
                     /locus_tag="ACHOQW_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576146.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHN"
     gene            complement(331..>437)
                     /locus_tag="ACHOQW_005302"
     CDS             complement(331..>437)
                     /locus_tag="ACHOQW_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFP9576147.1"
                     /translation="FKISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          131 a           85 c           88 g          133 t
ORIGIN      
        1 attgtgattg ttaaccactg cgtgcattgg tgggattgaa gggtgaaaaa tatgtgcaat
       61 tgggaagcct ttcattgatg cctgccttgt catcatgaca aacaataata gatgcttatt
      121 ttcctcgcag ggttctttcg cactatcaaa aaacgctcat ttacccggcg ttacagtaaa
      181 cgaagcaaca ttctgtggcc tacgccgatc aaaagctctg tgaaaatttc ccttgttgtc
      241 tttgtctgac cttatcgcgg cgatcagcaa ttattcgttt tgtgatcctc aacacaaaat
      301 gctattcgtc gcctatgaag gaagaagaat ttatggaaaa cgattgcatt cacaaaatga
      361 cgtcctgtca gaagagaaaa agttgacgtt aatcaattgt tcgagatgat caatacctgt
      421 attcgaaatt ttaaaat
//
LOCUS       DAZKFZ010000620          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000620 DAZKFZ010000000
VERSION     DAZKFZ010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.624"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..218)
                     /gene="cesF"
                     /locus_tag="ACHOQW_005303"
     CDS             complement(<1..218)
                     /gene="cesF"
                     /locus_tag="ACHOQW_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFP9576148.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCYLLNSESIQINSILEMNSNRELLG"
BASE COUNT          146 a           71 c           78 g          142 t
ORIGIN      
        1 ccgagcagtt ctcgattgct attcatttca agtattgaat taatctgtat tgattcgcta
       61 ttcaataaat aacaataaaa aaacagaatt tcagattgag atgcacactc cagtatccat
      121 tcatggccat cgttatcgtg gaaagagagc acgtcagttt gctcttcaag agctatccca
      181 aacgtatgtg caaggtctgt gcgaaatttc tcattcatat tgtagtcctg ccattctttt
      241 catgttgata tactcaaacg taattaatcc gagagttctg atacaaataa taaggcgtag
      301 atataagatt acgggaatag tgttgcatat tcatgatagc atatgataaa tgtagtgcaa
      361 ctacctgtag ataaggccgg aggataattc aaatataaat acaagataat tattctttaa
      421 agttattttt taatggg
//
LOCUS       DAZKFZ010000621          435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000621 DAZKFZ010000000
VERSION     DAZKFZ010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.625"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>435
                     /locus_tag="ACHOQW_005304"
     CDS             <1..>435
                     /locus_tag="ACHOQW_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576149.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVV"
BASE COUNT          120 a          121 c          111 g           83 t
ORIGIN      
        1 tcatggtgat tatcatcaca gcgaaactgt catacatcgt tacggtaccg gcgcgatggt
       61 gttgtgctgg cactgcgaca accagctgcg cgaccagaca tccgaatcac ttgagcaact
      121 tgctcaacag aatctggccg cctggatgat tgacgtcatc cgccacgcaa tgaatggcat
      181 acaggaacgg gaattatcgc tggctgaatt atcctggtgg gcagtctgca atcaggtggt
      241 ggacgcatta cctgaggcag tatcgcgtcg ttctctggga ttaccggcgg aaaaaatccg
      301 ctccgtatac cgtgaaagcg acatcatacc gggagaacag accgccacca gcatactgaa
      361 gcagcgcaca aaaaatattg cgctaccgcc tcacacccac cagcaacaga acccaccaca
      421 ggaaaagacg gtggt
//
LOCUS       DAZKFZ010000622          434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000622 DAZKFZ010000000
VERSION     DAZKFZ010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.626"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>434
                     /locus_tag="ACHOQW_005305"
     CDS             <1..>434
                     /locus_tag="ACHOQW_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9576150.1"
                     /translation="LQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELL
                     DYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHN
                     EKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKKNM"
BASE COUNT          161 a           76 c           79 g          118 t
ORIGIN      
        1 attacaaaat aaaagtgata tacagacgct aaaaacagaa tgttattgcc ttctagcgga
       61 atgccatatg tctttggcac ttcatggaaa aagtgaactt gaacttgctg ctcaaaaagc
      121 attagagctt ttagattatg tatcagacat aaccactgtc gatggaaaaa ttttagctat
      181 tatgggactg ataactggtc tgtctggaca agcaaaagta tctcatatct tatttgaaca
      241 ggctaagata cactcaactg atatagcctc tctctactac tatagggcac ttgtcaactt
      301 tcataatgaa aaaattgaag aggcaaggat ttgtatagac aaatcactac aactcgaacc
      361 cagaagacga aaagcagttg tgataaaaga atgtgtagat atgtatgtgc ctaacccgct
      421 caaaaaaaac atga
//
LOCUS       DAZKFZ010000623          429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000623 DAZKFZ010000000
VERSION     DAZKFZ010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.627"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>429
                     /locus_tag="ACHOQW_005306"
     CDS             <1..>429
                     /locus_tag="ACHOQW_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9576151.1"
                     /translation="PEAVYARLHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGS
                     FFTAGATMALWGAALSAGGLTATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGK
                     QNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRD"
BASE COUNT           88 a          115 c          142 g           84 t
ORIGIN      
        1 accggaggcg gtgtacgccc gtcttcacga acctctgggt gagggggcgg tcatccatat
       61 tgtgccgcga ctggccggag ccggaaaggg cggactgcag attgtgctgg gggcagcagc
      121 catcgtgggc tctttcttca ccgccggcgc aacgatggcg ttgtggggcg cagccctgag
      181 tgccggaggg ctgactgcca ccacgatgct gttctcactg ggtgccagca tgatactggg
      241 cggtgtggcc cagatgctgg caccgaaggc aaaaacaccg gattaccgcg caacggataa
      301 cggtaaacag aacacgtatt tttcgtcact ggataacatg attgctcagg ggaacccgat
      361 gccggtgcct tatggtgaaa tgctggttgg ttcacgacgg atatcccagg acatcagcac
      421 ccgtgatga
//
LOCUS       DAZKFZ010000624          429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000624 DAZKFZ010000000
VERSION     DAZKFZ010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.628"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..101
                     /locus_tag="ACHOQW_005307"
     CDS             <1..101
                     /locus_tag="ACHOQW_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9576152.1"
                     /translation="DKILKRTLYDKNDGNINADKWYEFHKKIKKGE"
     gene            102..>429
                     /locus_tag="ACHOQW_005308"
     CDS             102..>429
                     /locus_tag="ACHOQW_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576153.1"
                     /translation="MAKHINGYHEKKIVDVINTWSIDEKLTWNALCDRLVRVIGKRPS
                     RQSLSSHVRIAESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERY
                     LEQFKVW"
BASE COUNT          180 a           53 c           86 g          110 t
ORIGIN      
        1 acgataaaat tttaaaaaga acactgtatg ataaaaatga tgggaacata aatgctgata
       61 aatggtatga attccataaa aaaataaaaa aaggagaata aatggctaag catataaatg
      121 gttatcatga gaagaagatt gtagatgtta ttaacacatg gtctatagat gaaaaactaa
      181 cctggaatgc tttgtgcgat aggttagtca gggttattgg gaaaagacca tcaagacaat
      241 ccttaagtag tcatgtaagg attgcggaaa gcttcaatgt gaaaaaaacc acaatcaaat
      301 ctggtgtaat acacactgta aaacctgcta atttaaaagt ggcttctcaa cgaattaaaa
      361 gacttgaagc agaaaatgaa gctttgaagg cattaaatga acgctattta gagcagttca
      421 aagtatggc
//
LOCUS       DAZKFZ010000625          429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000625 DAZKFZ010000000
VERSION     DAZKFZ010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.629"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            1..429
                     /locus_tag="ACHOQW_005309"
                     /pseudo
     CDS             1..429
                     /locus_tag="ACHOQW_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          106 a          106 c          154 g           63 t
ORIGIN      
        1 gcagcgtcgc gatttatgga aagcggaaaa tcagtatgcg gtcctgaaag aggctgccac
       61 gaaacggcag ttatctgagc aggaaaaatc cctgctggcg cataaagacg agacgctgga
      121 gtacaaacgc cagctggctg agctgggaga caaagttgaa caccagaaac ggctgaatga
      181 gctggcacag caggctgcgc ggtttgagca gcagcagggc gcgaagcagg cggcaatcag
      241 tgcccaggcg cgggcctcac cgaccgtcag gcgcagcggg agtcggaaga gcagcgcctt
      301 cgtgacgtgt acggtgataa tccggatgcg ctggcgaagg ccacatctgc actgaagaac
      361 acctggtctg cggaggagca gcttcgtgga agctggatgg ccgggctgaa gtccggctgg
      421 ggcgagtgg
//
LOCUS       DAZKFZ010000626          423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000626 DAZKFZ010000000
VERSION     DAZKFZ010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.630"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..358)
                     /locus_tag="ACHOQW_005310"
     CDS             complement(<1..358)
                     /locus_tag="ACHOQW_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP9576154.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIK"
BASE COUNT          147 a           62 c           78 g          136 t
ORIGIN      
        1 ctttaataat ggcaatttgc tttaatgaat atgaaacaca taaccgcagg atatctatct
       61 catcatgggt aagtggttct tgtgtggaat acacattttg tgtatgaaga tttgaaacca
      121 ggaaatgcgt taaatcagat aatgtaatat tcttattgat ccagatgaaa ttgttactca
      181 aaaggatgtt tacaggttct gtatttggtt tgagatttcg atgttgatgc tcatcaataa
      241 caacgaacga atgtccaaga tcaaccaacg caactaaatc gctgtaatat tccttttggg
      301 atgctaataa tgcattagtg cctacagtga agtagacatc actggaaaag acttgcatat
      361 gccaacctgt actctaaaat catttagggt aaatattatg tgtaaaattt aaaataagag
      421 taa
//
LOCUS       DAZKFZ010000627          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000627 DAZKFZ010000000
VERSION     DAZKFZ010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.631"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(77..>421)
                     /locus_tag="ACHOQW_005311"
     CDS             complement(77..>421)
                     /locus_tag="ACHOQW_005311"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9576155.1"
                     /translation="TGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALIISFWL
                     DVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAVLITIL
                     VAIIIWGVLLLS"
BASE COUNT          141 a           93 c           71 g          116 t
ORIGIN      
        1 gaggataaga ggtgaatacc tcttatcctt aagtaaatat taaccaggta actgtgaata
       61 atttagatgt tgggcgttat gaaagtaata aaactcccca tataatgata gcaactaaaa
      121 tcgtaatcaa cactgcggct gaacccatat cttttgctct gcctgataat tcgtgatgtt
      181 ctgaaccaat tctgtcaaca actgcttcaa ttgcactgtt aattatttca acaataatta
      241 ccatcattgc cgaaccaatg agtaatactc gagtaacaga gtcaacatcc agccaaaagg
      301 atattatcag tgctgacaga accagaatgc actcctgacg aaaggctgct tcatagcgcc
      361 atgccgcaca taagcctttc catgaataac ccgctgcttt aataattctg gtaacaccag
      421 t
//
LOCUS       DAZKFZ010000628          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000628 DAZKFZ010000000
VERSION     DAZKFZ010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.632"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>418
                     /locus_tag="ACHOQW_005312"
     CDS             <1..>418
                     /locus_tag="ACHOQW_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9576156.1"
                     /translation="SFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCID
                     VERKRTFTMMIREGVAMHAFNGELFVQATWDTRPSRLFRTQFRMVSPKRISNPNNTSD
                     SRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTW"
BASE COUNT           90 a          109 c          131 g           88 t
ORIGIN      
        1 ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag
       61 aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaagatgact
      121 gttgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg
      181 ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc cgtccctcgc
      241 gactgttccg gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata
      301 ccagcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg
      361 gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggat
//
LOCUS       DAZKFZ010000629          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000629 DAZKFZ010000000
VERSION     DAZKFZ010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.633"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>418)
                     /locus_tag="ACHOQW_005313"
     CDS             complement(<1..>418)
                     /locus_tag="ACHOQW_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576157.1"
                     /translation="TIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHY
                     NDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLINLKTLLNDGCFI
                     KYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFE"
BASE COUNT          141 a           76 c           63 g          138 t
ORIGIN      
        1 ttttcaaaac aacatttttt caatatgctt tcattcacaa cagtgtgtga taaatagaga
       61 ttacttatgt tttgctttat taatgccacg tcattaaaat gactatattt aataaagcaa
      121 ccatcattga gtagtgtttt aagattgatt aaattacttg catcttcacg taccatcgtg
      181 gcaactggaa caggcagaga catgaaaact tcttcattca ccatcaagtg aatcatgcat
      241 tgcgttccct ttgtcatatc cacacttacg ctacaaatat cgttgtaatg cgaagagtgc
      301 atttcaggaa acagattaaa cacggtatct tgaaaggcac taaatcgtgt taataaatcc
      361 tgataatgaa caggttttgt ttcaaaatta tcatcagcaa atccaagcac tattgtat
//
LOCUS       DAZKFZ010000630          416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000630 DAZKFZ010000000
VERSION     DAZKFZ010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.634"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..309
                     /locus_tag="ACHOQW_005314"
     CDS             <1..309
                     /locus_tag="ACHOQW_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576158.1"
                     /translation="GRFLSVSCFYDKPFIKKHFFEIVMIAFVAYFWLFSFLTGYYKPQ
                     FKKEYEMINYNDGWYYVLARYDNCLVLSTSFNAGSKRFVIYQSAQDKNLQVDIVRTRI
                     "
BASE COUNT          121 a           53 c           81 g          161 t
ORIGIN      
        1 gggcgatttt tatcggtatc atgcttctat gataagccat ttataaaaaa acattttttt
       61 gagattgtga tgattgcttt tgtggcatat ttctggcttt tttcatttct gacaggatat
      121 tacaaaccac agtttaagaa agaatatgaa atgattaatt ataatgatgg ttggtattat
      181 gttcttgctc gttatgataa ttgtctggtt ttgtctactt ctttcaatgc aggtagtaaa
      241 aggtttgtca tttatcaatc agcacaagat aagaatcttc aggttgatat tgtaaggacc
      301 agaatttaat tggctgcata aataatattt taagttgcaa gttggctatt cgtaggaata
      361 gaaccttagg catgctgaat gcgtttcctg aacattgttt tataaactgt gtctgc
//
LOCUS       DAZKFZ010000631          413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000631 DAZKFZ010000000
VERSION     DAZKFZ010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.635"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>413)
                     /locus_tag="ACHOQW_005315"
     CDS             complement(<1..>413)
                     /locus_tag="ACHOQW_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9576159.1"
                     /translation="SLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERILL
                     FLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLE
                     ERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISV"
BASE COUNT          125 a          105 c           73 g          110 t
ORIGIN      
        1 aatacagaaa taaagtagga ccagcttgaa taaccgcatt tttctgctac agcctgtcca
       61 tatccatgcc gggaacataa catattgaca gcaacacgca tccgctcttc cagcaacaag
      121 cgactgaaca tgtgcccttc atttttcagt tttatcttta acaaactctc actcatatgc
      181 aggcgtaacg caatcgcacc aagcgtccag cttgctgata tatctgtctg aattatcgcc
      241 ctgactttgg cacttatact ggataaacat ccacttaaaa ataataatat ccgttcatct
      301 gattcaaaca gcgacaggca tgccatcata agaaacatat ccgtggtatc tccggaaaat
      361 ctctggctgg taattaaagc ctcagccaac gcagggttgt tgggttccag tga
//
LOCUS       DAZKFZ010000632          412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000632 DAZKFZ010000000
VERSION     DAZKFZ010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.636"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>412
                     /gene="espJ"
                     /locus_tag="ACHOQW_005316"
     CDS             <1..>412
                     /gene="espJ"
                     /locus_tag="ACHOQW_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFP9576160.1"
                     /translation="VRIQNNQFTDLKNKKIQGHQNTVASVMDWYNPQKNALGITMGTP
                     RKSADIAKEEHRNALNFMIMEKNTFHEKILNSNDNLQKSYSKTEDSSWVAASVGSLLD
                     KGAKVYPDTSCSLRLGEPFIFTLPESVRVDVDIYP"
BASE COUNT          161 a           78 c           74 g           99 t
ORIGIN      
        1 tgtaagaatc caaaacaatc agtttactga tttgaaaaac aaaaaaattc aaggccatca
       61 aaacacagtt gccagtgtca tggattggta taatccccaa aaaaatgctt tgggtataac
      121 tatgggtaca ccaagaaaat cggccgatat agcaaaagaa gagcacagaa atgcactaaa
      181 tttcatgata atggaaaaaa acacatttca tgaaaaaatc ttaaacagca atgacaattt
      241 gcaaaaatca tacagcaaaa cagaagacag tagttgggtc gcagcttctg tcggctctct
      301 tctagataaa ggagcaaaag tatatcccga tacatcatgc tctctgagac tgggagaacc
      361 attcatcttt acgctgccag aatcagttag agtcgatgtt gacatatatc ct
//
LOCUS       DAZKFZ010000633          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000633 DAZKFZ010000000
VERSION     DAZKFZ010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.637"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(73..270)
                     /locus_tag="ACHOQW_005317"
     CDS             complement(73..270)
                     /locus_tag="ACHOQW_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9576161.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
BASE COUNT          108 a          116 c           88 g           99 t
ORIGIN      
        1 acgcatgagc agatggctgg tgagtgcccc taacacccac cagtcgccca tttaccacaa
       61 ataaaaaagc cttcaggact gaaggcgtct gtaacaaccg aactgatagt ctgccagacc
      121 cgccataaca agctgggtca gtattaactg gcagcgttcg cgtgaaaggt aagtattctg
      181 cgcaatctcc ccgactgtcg ccggttcggt gacgcttaat tcattaaaca ctgctctggc
      241 agtttctgtc atatcctgct gtttcagcat gtctttttcc ctttttcggt taacgtgaca
      301 caccaataac tcttgtcaaa aaagccagca agctgaaaga ccggtattca ccgccaccag
      361 cgcgtttact gtactgacgc gatttcagtc ataaaaaacc cgccaggcgg c
//
LOCUS       DAZKFZ010000634          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000634 DAZKFZ010000000
VERSION     DAZKFZ010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.638"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>411)
                     /locus_tag="ACHOQW_005318"
     CDS             complement(<1..>411)
                     /locus_tag="ACHOQW_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9576162.1"
                     /translation="NNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIKE
                     RNFFTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTAY
                     FGDTDGRVGAVLYALLVSGHIGIREKGWSLLCDL"
BASE COUNT          139 a           89 c           60 g          123 t
ORIGIN      
        1 ataaatcaca taataaactc cacccctttt ccctgatgcc tatatgccct gaaacgagca
       61 atgcataaag tacagcgcct actcttccat ctgtatcgcc aaaataagcc gtgttggcat
      121 tttgtgggtt aatcacacca cactgtttat aaaagtctat ttttgaaaaa acagattcaa
      181 catagctatc aaacttttcc ttagtaaacg tctctttaga gacagcccaa cgccagtcag
      241 gtttgaaatt accactaaga agagtaaaga aatttctctc tttgatagaa aacccatcaa
      301 ctttataaat tatgtctgat ttatccccgg agaaattaat aaaataataa ccgtccttac
      361 gctcaatcgt aacggtaatg ggagacgata attttatcac atggttattg t
//
LOCUS       DAZKFZ010000635          406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000635 DAZKFZ010000000
VERSION     DAZKFZ010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.639"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..243
                     /locus_tag="ACHOQW_005319"
     CDS             <1..243
                     /locus_tag="ACHOQW_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9576163.1"
                     /translation="AEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRW
                     QLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            240..>406
                     /locus_tag="ACHOQW_005320"
     CDS             240..>406
                     /locus_tag="ACHOQW_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9576164.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTG"
BASE COUNT          113 a           95 c          129 g           69 t
ORIGIN      
        1 gctgaagagc aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa
       61 aagcgacgca atgaggcgct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt
      121 atttcccgct ggcagctgga tctcagtgca ctgctggcga gcctgcagga agaggatggt
      181 gcagcaacca acaagaaaac actggcagaa tacgcccgtg ccttatccgg agaggatgaa
      241 tgacgcgaca ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa
      301 aacgggtggc aacggtacag aaagacggac ggcgagtgga gtttacgacc acttccgtgt
      361 ctgacctgaa aaaatacatt gctgagctgg aagtgcagac cggcat
//
LOCUS       DAZKFZ010000636          404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000636 DAZKFZ010000000
VERSION     DAZKFZ010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.640"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(77..343)
                     /locus_tag="ACHOQW_005321"
     CDS             complement(77..343)
                     /locus_tag="ACHOQW_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP9576165.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
BASE COUNT          124 a           70 c           65 g          145 t
ORIGIN      
        1 taatttaaaa aattaaacat gtatcgtagt caaaaattta taagaatcta cacgcaaaaa
       61 acgtgtagat ttttatttaa tgagttaaac gatcttgcga aatgacaagc aattgataca
      121 tactcatctc tttataagct ggaactgtag taacctgtgc aaattcgcga tatagctttt
      181 cctccagttc tgctgatgat atatattccg ggtgttcgct aaagaactgt cttgcatcta
      241 ctatcgcttg ggattcgcca cttcttttat tttgattctc ctttaatgaa ttttctctgg
      301 tgcatgcaga gagcaacgat atcagcgcga tggttattat gattttacta ttatatcatt
      361 actaatgaca tatcaacttt ctcttacgca agattggagt ttaa
//
LOCUS       DAZKFZ010000637          403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000637 DAZKFZ010000000
VERSION     DAZKFZ010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.641"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>403)
                     /gene="espR4"
                     /locus_tag="ACHOQW_005322"
     CDS             complement(<1..>403)
                     /gene="espR4"
                     /locus_tag="ACHOQW_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFP9576166.1"
                     /translation="TNIAELLDKLTKITDARLCKGFSDWASSVKEGGSNDLKENVDRA
                     LVRMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNELDSIQVPESCKELELDF
                     NNLTEFPQVPDGITLISVNNNLISYIDSFPPK"
BASE COUNT          120 a           80 c           75 g          128 t
ORIGIN      
        1 ctttggcgga aatgagtcaa tatacgatat caggttatta tttacggaga tcagggtaat
       61 tccatcaggt acttgtggaa attctgtaag gttattgaaa tcgagttcta attctttgca
      121 tgattcggga acctgtattg aatcaagttc attataaaca aggctaagca tttcaatcca
      181 atctggaaga ggcggcacag aacccaaaaa taggtatgat aagtcaagtt cattactgtg
      241 aagcttaaca catttaaaca ttctcaccaa cgctctatct acattttctt tgaggtcatt
      301 cgatccgcct tctttgactg aactagccca gtcactgaag cctttgcaaa gtctggcatc
      361 tgtaattttc gttaacttgt ccagcaactc tgcaatatta gtc
//
LOCUS       DAZKFZ010000638          402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000638 DAZKFZ010000000
VERSION     DAZKFZ010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.642"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            165..371
                     /locus_tag="ACHOQW_005323"
     CDS             165..371
                     /locus_tag="ACHOQW_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP9576167.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          120 a           80 c           93 g          109 t
ORIGIN      
        1 gtgtaactta attacaggat gaatgtaaat aaaccatcaa taagcaaaaa taagtggtcg
       61 gatgagtagt aaaattacac aaaaggcggt actattttca tcagataaac aaattcattt
      121 ttccggtgct attcaccggg tagatacaaa agggggagaa aagtatggaa ctctacagag
      181 aatatcctgc atggcttatc tttttacgcc gtacttatgc ggttgcagcg ggcgttctgg
      241 cgctgccttt catgcttttc tggaaagacc gcgcccgctt ttacagctac ctgcatcgcg
      301 tctggtcgaa aaccagcgat aaaccggttt ggatggatca ggccgaaaag gcaaccggtg
      361 atttttactg attgctgagt tttgaataca ttgaaaaccg cc
//
LOCUS       DAZKFZ010000639          401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000639 DAZKFZ010000000
VERSION     DAZKFZ010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.643"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>401
                     /gene="wzx"
                     /locus_tag="ACHOQW_005324"
     CDS             <1..>401
                     /gene="wzx"
                     /locus_tag="ACHOQW_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFP9576168.1"
                     /translation="ISLSVISSLISKILSLLSLILTVSLTLPYLGQERFGVWMTITSL
                     GAALTFLDLGIGNALTNRIAHSFACGKNLKMSRQISGGLTLLAGLSFVITAICYITSG
                     MIDWQLVIKGINENVYAELQHSIKVFVIIFG"
BASE COUNT          119 a           59 c           79 g          144 t
ORIGIN      
        1 ttatatcctt gtctgttatt tcaagtttga ttagtaaaat actctcacta ctttctctta
       61 tattaactgt aagtttaact ttaccttatt taggacaaga gagatttggt gtatggatga
      121 ctattaccag tcttggtgct gctctgacat ttttggactt aggtatagga aatgcattaa
      181 caaacaggat cgcacattca tttgcgtgtg gcaaaaattt aaagatgagt cggcaaatta
      241 gtggtgggct cactttgctg gctggattat cgtttgtcat aactgcaata tgctatatta
      301 cttctggcat gattgattgg caactagtaa taaaaggtat aaacgagaat gtgtatgcag
      361 agttacaaca ctcaattaaa gtctttgtaa tcatatttgg a
//
LOCUS       DAZKFZ010000640          399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000640 DAZKFZ010000000
VERSION     DAZKFZ010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.644"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(177..>399)
                     /locus_tag="ACHOQW_005325"
     CDS             complement(177..>399)
                     /locus_tag="ACHOQW_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576169.1"
                     /translation="AVISLKRLESSNREFIRYRFAQCSFTLTWGARVGRLAFSRLYPC
                     YTELNKIRAMQLNNAYISDFQNPDEISSK"
BASE COUNT          131 a           63 c           75 g          130 t
ORIGIN      
        1 taaatctaca aagcaataat ctacgtagaa cataaatcta cgtatatctt ccggtatagc
       61 agtggaaaaa taacggtatt gcgtgtaagt ggtttgatca tcgaattcgt ctgattgtac
      121 tgaatatgta aatgtggcca gtataaatag taatgttaat cttaaattta taataatcat
      181 ttgctggata tctcatctgg attttgaaag tcagaaatgt aagcattgtt tagttgcatg
      241 gctcttattt tgtttaactc agtatagcat ggatatagcc gagagaatgc cagtcgccct
      301 actctggccc cccaggttag agtaaaagag cattgagcaa atctgtaacg tataaattcc
      361 ctattagaag actctagtct ttttaaagaa ataactgct
//
LOCUS       DAZKFZ010000641          398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000641 DAZKFZ010000000
VERSION     DAZKFZ010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.645"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>398
                     /locus_tag="ACHOQW_005326"
     CDS             <1..>398
                     /locus_tag="ACHOQW_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9576170.1"
                     /translation="FKSWFVDFDPVIYNALDAGNPIPEALQSRAELRQKVRNSTDFKP
                     LPAEIRSLFPSEFEETELGWVPKGWSIVRTEDIALKIGMGPFGSNIKVSTFVNAGVPI
                     ISGQHLKALLLIDGDNNFITPEHAEKLKNSA"
BASE COUNT          117 a           77 c           89 g          115 t
ORIGIN      
        1 ttcaaatcct ggtttgtgga ttttgatccg gtgatttata acgctctgga tgcaggaaat
       61 ccaatcccgg aagctctgca atctcgtgcc gaattacgtc aaaaagtacg taatagtaca
      121 gattttaaac cgcttccggc ggaaatccgt tcgcttttcc caagtgaatt tgaagaaacg
      181 gagttgggtt gggtgccgaa aggatggagt attgttcgaa ctgaagatat tgcattgaaa
      241 ataggaatgg gaccatttgg ttccaatatt aaagtatcca catttgttaa tgctggtgta
      301 ccaattataa gcggccaaca tctgaaagcc ctccttctta tcgatgggga taataatttc
      361 attactccag agcatgctga aaagctcaaa aactctgc
//
LOCUS       DAZKFZ010000642          394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000642 DAZKFZ010000000
VERSION     DAZKFZ010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.646"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..371
                     /locus_tag="ACHOQW_005327"
     CDS             3..371
                     /locus_tag="ACHOQW_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9576171.1"
                     /translation="MGEAAKRDVGTGENQIPDMASFASGDGWMKLPNGKILQYGRGAV
                     TPTLSTQTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVMTAEGPTLTGFNSAY
                     RTSSTSDTVSMNYSWWAVGE"
BASE COUNT          101 a           80 c          113 g          100 t
ORIGIN      
        1 gtttgggaga agcggcaaaa cgggatgtgg ggacagggga aaatcagata ccggacatgg
       61 cctcttttgc cagtggtgat ggatggatga aattacccaa cggtaaaatc ctgcaatatg
      121 gtcgtggtgc ggttacgccg acattatcga cgcaaacaat gagaattaca ttcagcatcc
      181 ctttccccaa aaaagcggac tgcgccatgc ttactcattc tggtgatggc ggtgcgcctt
      241 taggcgctgg gcgagggttc gtgatgactg cagaaggccc aacgttaacc ggctttaatt
      301 ctgcttacag aacgtcatca accagcgaca cggtatcgat gaattacagt tggtgggctg
      361 ttggtgagta attttattta gggtgattta tatg
//
LOCUS       DAZKFZ010000643          391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000643 DAZKFZ010000000
VERSION     DAZKFZ010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.647"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            71..391
                     /locus_tag="ACHOQW_005328"
                     /pseudo
     CDS             71..391
                     /locus_tag="ACHOQW_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          131 a           68 c           78 g          114 t
ORIGIN      
        1 aacaaatgtg ccttatataa aaaggagaac aaaatggaac ttaaaataat tgagacaata
       61 gatttttatt atccctgttt acgatattat agccaaagtt gtatcctgca tcagtcctgc
      121 aatatttcac gagtgctttg ttaactgaat acatgtctgc cattttccag atgataacga
      181 cgtcatcgca attgatggta aaacacttcg gcacacttat gacaagagtc gtcgcagagg
      241 agtggttcat gtcattagtg cgtttcagca atgcacagtc tggtcctcgg atagatcaag
      301 acggatgaga aacctaatgc gttcacagtt attcatgaac tttctaaaat gatgggtatt
      361 aaaggaaaaa taatcataac tgatgcgatg g
//
LOCUS       DAZKFZ010000644          378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000644 DAZKFZ010000000
VERSION     DAZKFZ010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.648"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            3..>378
                     /locus_tag="ACHOQW_005329"
     CDS             3..>378
                     /locus_tag="ACHOQW_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576172.1"
                     /translation="MQPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVVNI
                     PDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNVTQ
                     MALEIPSSVSGISGKYGVQLNIV"
BASE COUNT          134 a           84 c           65 g           95 t
ORIGIN      
        1 acattcaacc gaccatacaa tctggaatca cctcacaaaa caatcaacat catcaaacag
       61 aacaaatacc ctctacacaa ataccgcaat ccgaattacc tctaggatgc caagctggat
      121 ttgttgttaa tattccagat gatatacagc aacatgcacc ggaatgcggt gaaacaacag
      181 ctctactgag cttgataaaa gataaaggtc tgctctcagg gctagacgaa tatatagctc
      241 ctcaccttga agaaggatcc ataggaaaaa aaacattgga tatgtttggt ttattcaatg
      301 ttacccaaat ggcattagag atacctagtt ccgtttcagg catctctggt aaatatggtg
      361 tccagctaaa cattgtaa
//
LOCUS       DAZKFZ010000645          371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000645 DAZKFZ010000000
VERSION     DAZKFZ010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.649"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            18..>371
                     /gene="espM1"
                     /locus_tag="ACHOQW_005330"
     CDS             18..>371
                     /gene="espM1"
                     /locus_tag="ACHOQW_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFP9576173.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTL"
BASE COUNT          115 a           57 c           91 g          108 t
ORIGIN      
        1 aatgaaaagg atttgttatg ccagtaaatg cgacaggtgt gtccttcagc tcttttggta
       61 tcagttatca taaagataat tctttccggg gcaccatccg tgggaagaat gatgaggtcg
      121 tgaagtgttc aatgggagag cgctctatcc gctttaatgt taacaaattt agcggctgta
      181 tactggagac ggtaagcagg cagagtacta aagatattca tggatgggta agtgatgaac
      241 ggacagtata tccgtcaagg gttatcaacc aagagattga taattgctgc cttcaaaaaa
      301 atgcaaaaat ttcttctgaa gagaggaaaa tggtcttttc tcttgtaagt aaggagtttg
      361 aactaactct t
//
LOCUS       DAZKFZ010000646          371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000646 DAZKFZ010000000
VERSION     DAZKFZ010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.650"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>371
                     /locus_tag="ACHOQW_005331"
     CDS             <1..>371
                     /locus_tag="ACHOQW_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576174.1"
                     /translation="KLKKESGYKKELDERLDELRELIDEESQSMITPFLTQRDFYIKE
                     ISKNEKVREQLVKDLKVRNQQEELLEKYQVLRKDIEALTERLEALKKNAPSMEGILQG
                     LGDRFSQYLKGINIKNRTGIK"
BASE COUNT          152 a           40 c           89 g           90 t
ORIGIN      
        1 taaattaaaa aaagaatcag gttataaaaa ggaattagat gaacgacttg acgagttgag
       61 agaattgatt gacgaagaaa gccagtcaat gattacacca ttcttgactc agagagattt
      121 ttatattaaa gagatctcaa aaaatgagaa ggttagggag cagttagtaa aagatttgaa
      181 ggttagaaac cagcaagagg aactgcttga aaagtatcag gtactgagaa aagatattga
      241 agcattaact gaacgcttgg aggcgttaaa gaaaaacgca cctagcatgg agggcatttt
      301 acaaggtttg ggtgatagat ttagtcagta tttgaagggt attaatatca aaaacagaac
      361 ggggataaaa a
//
LOCUS       DAZKFZ010000647          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000647 DAZKFZ010000000
VERSION     DAZKFZ010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.651"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..115
                     /locus_tag="ACHOQW_005332"
     CDS             <1..115
                     /locus_tag="ACHOQW_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576175.1"
                     /translation="DYYNHNNETKSTIDNYISKIKDNHNTPWLLNNENVQE"
BASE COUNT          157 a           74 c           46 g           92 t
ORIGIN      
        1 tgattactat aaccacaata atgaaacgaa atccactatt gacaattaca tatcaaaaat
       61 aaaggataat cataataccc catggttatt aaacaacgaa aatgttcagg aataaaaaaa
      121 aagagacaat tcccgagata aaacaaaaga gcataaacat atgaaccgga aattctattt
      181 caaaaatatc ctgtggggaa tgtgccccat tataatatcc atattgcatc tgatatatga
      241 cacaccaaca gaagcatcga taaaagttga cttatttctg atactcagtg gcctggccct
      301 tccttgttca atatacctga caaaaacaat cacactaaaa tacacatcca tatcattctg
      361 gaaaaaacc
//
LOCUS       DAZKFZ010000648          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000648 DAZKFZ010000000
VERSION     DAZKFZ010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.652"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          113 a           75 c           56 g          124 t
ORIGIN      
        1 gatcacccga aactgcaggg tattgcccag cagaacagct ttaaacacac ctgataacat
       61 aacgttgtaa aaaccgaatg cccagccttt aaaaaaaaca gctgggcatt cggttgctta
      121 ttaatcgcaa taatatattg gctgtctatt catcgtgttg ataagatata tgttatatga
      181 atgtttatta aatgtagcga ttacctttaa tgtcacgaca agggattatt tgcttactat
      241 taatttccct gcgttcttaa acacgttttt gtattataaa tccagtcatc ctgcccgtag
      301 aacatcacac attatcatcc tgttctcccg cttcgtttaa atttatttat caatcaattt
      361 gacttaag
//
LOCUS       DAZKFZ010000649          367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000649 DAZKFZ010000000
VERSION     DAZKFZ010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.653"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            172..357
                     /locus_tag="ACHOQW_005333"
     CDS             172..357
                     /locus_tag="ACHOQW_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP9576176.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVIGTFIKKHIFY"
BASE COUNT          117 a           73 c           65 g          112 t
ORIGIN      
        1 aagtatctat cacggcactc tcagttgagt atcacaggca catgtatttt agccccctga
       61 gtctacagac aaccaatatt acttaaataa gtgataatct taatgctgtt ttttagaaca
      121 gatgattgat atttttgggc cggagaaacg cagacaggct ttgttgaata aatcgagctt
      181 ttgctgagtt taaggatcag atcactcatt ttcctgcaaa agctcattgg gcgaaacaat
      241 ctgactcaga gaacccggat taaacgtctg gcccgcagaa tcatctattt ctcacgcact
      301 gtagtacgta ataaaaaagt catcggtact tttatcaaaa aacatatatt ctattaatta
      361 ttgccat
//
LOCUS       DAZKFZ010000650          366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000650 DAZKFZ010000000
VERSION     DAZKFZ010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.654"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>366
                     /locus_tag="ACHOQW_005334"
     CDS             <1..>366
                     /locus_tag="ACHOQW_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576177.1"
                     /translation="SDLVRRKMNDLPESPTGNNLEALAPGIEKLKQTSIEMVTLLNTL
                     QPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDNTCELTINEPLK
                     NQEISTGNININCPLKDIY"
BASE COUNT          137 a           70 c           54 g          105 t
ORIGIN      
        1 tagtgattta gtccgtcgaa aaatgaatga cctacctgaa agcccaacag gaaataacct
       61 cgaagcacta gcaccaggta ttgaaaaact aaaacagacc tctattgaaa tggtcacttt
      121 acttaacacg ttacaaccta gtggaaaatg cattatcact ggtgattttc aaaaagaatt
      181 agcgtactta caaaatgtaa ttctttataa tgactcgtct cttcgtctgg attttttagg
      241 ttataacgcc caaattattc aacgatcgga caatacttgt gaacttacca ttaatgaacc
      301 gttaaaaaac caggaaatat ccacaggtaa tatcaatatt aattgcccat taaaagatat
      361 ttacaa
//
LOCUS       DAZKFZ010000651          365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000651 DAZKFZ010000000
VERSION     DAZKFZ010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.655"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(71..>365)
                     /locus_tag="ACHOQW_005335"
     CDS             complement(71..>365)
                     /locus_tag="ACHOQW_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576178.1"
                     /translation="LIHGAGLDKDASVAIGTAAGGFLGKATKIPGADIAGSAIGGYIG
                     GVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSSSSSGS"
BASE COUNT          115 a           79 c           53 g          118 t
ORIGIN      
        1 tacaaattag gataatatat cactaaaagc agcattgctg cataataagc tatgctgctt
       61 tagaaattaa tcaacttcca ctactagaag aggatgttga tgagttaaat cctgagttta
      121 ataaacctcc attgtagttt ggattaaaac ttccaagtcc tatacccgga ttatagttaa
      181 taccgtttga tggtattgtt attgtcctat ttgaaccatc agcaataact cctccaatat
      241 atcctccaat tgcacttcct gctatatccg caccagggat ttttgttgct ttacctaaaa
      301 aaccaccagc cgcagtacct attgcaacgg atgcatcttt atctaatcct gcaccatgta
      361 ttaaa
//
LOCUS       DAZKFZ010000652          363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000652 DAZKFZ010000000
VERSION     DAZKFZ010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.656"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..203)
                     /locus_tag="ACHOQW_005336"
     CDS             complement(<1..203)
                     /locus_tag="ACHOQW_005336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576179.1"
                     /translation="MCRKYAHVVDALQGNFPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYRYDEYGRLAEKTDR"
BASE COUNT           76 a           93 c           85 g          109 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccatgccgtg agggtgctgt ccgggtgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtctgttgca tacgggaaat tcccttgcag ggcgtccaca
      181 acatgtgcat atttcctgca catgctgtgg ttttgtcata caaggagcaa caatactagt
      241 taatgattca taaaaaattt taacggggtg atttttcatg cgattatcgt cctaattaac
      301 gccacctcac ttacagatta tttcattcgt tacaatcttt tgattgcatt acacccaggc
      361 tta
//
LOCUS       DAZKFZ010000653          363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000653 DAZKFZ010000000
VERSION     DAZKFZ010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.657"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>363)
                     /gene="rfbE"
                     /locus_tag="ACHOQW_005337"
                     /gene_synonym="perA"
     CDS             complement(<1..>363)
                     /gene="rfbE"
                     /locus_tag="ACHOQW_005337"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFP9576180.1"
                     /translation="HPCDMEQIVELAKSRNLFVIEDCAEAFGSKYKGKYVGTFGDIST
                     FSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQYWHDVIGYNYRMTNICA
                     AIGLAQLEQADDFISRKRE"
BASE COUNT          113 a           78 c           49 g          123 t
ORIGIN      
        1 ttcacgtttt cgtgatataa aatcatcagc ttgttctaac tgggctaatc ctatagcagc
       61 gcagatattt gtcatcctat aattgtagcc tataacgtca tgccaatatt gcctatgtac
      121 agctaatcct tggcctttaa aatgtaaaca acggtcataa agtgttttgt cattcgtgac
      181 aaccattcca ccttcacctg tagtaatagt tttatttcca aaaaagctaa aagtagaaat
      241 atctccaaat gttcccacat atttaccttt atatttagaa ccaaaggctt cagcgcaatc
      301 ttcaattaca aacaaatttc tacttttggc cagttctaca atttgttcca tatcacatgg
      361 atg
//
LOCUS       DAZKFZ010000654          361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000654 DAZKFZ010000000
VERSION     DAZKFZ010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.658"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>361
                     /gene="espX5"
                     /locus_tag="ACHOQW_005338"
     CDS             <1..>361
                     /gene="espX5"
                     /locus_tag="ACHOQW_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFP9576181.1"
                     /translation="KDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSHLSLKGIV
                     IKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSYFAQIKMTD
                     GTLNSCSAMHVQFYNATM"
BASE COUNT          127 a           60 c           60 g          114 t
ORIGIN      
        1 aaagataagg aagtgtcaat taaggtggta tattttataa ataatgtcac cgtccataat
       61 aatactatcg aaatcccaca gacagtgaat ggtggttacg atttttcaca ccttagcctg
      121 aaaggtatcg tgattaaaga tgaagattta tccaattcga attttgcagg ttgcagacta
      181 caaaacgcta ttttccagga ctgtaatatg tataaaacga attttaattt cgccataatg
      241 gaaaaaatac tttttgataa ttgtattctc gatgactcat atttcgctca gataaaaatg
      301 actgacggaa ctctaaattc atgctccgct atgcatgttc aattctacaa tgcaacaatg
      361 a
//
LOCUS       DAZKFZ010000655          355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000655 DAZKFZ010000000
VERSION     DAZKFZ010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.659"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..147
                     /locus_tag="ACHOQW_005339"
     CDS             <1..147
                     /locus_tag="ACHOQW_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9576182.1"
                     /translation="GNEETNLWTLCTECHRQKSAREAAGGMPDPTLPEVSGGHGRADD
                     IIGL"
BASE COUNT           83 a           87 c          103 g           82 t
ORIGIN      
        1 ggtaatgagg agacgaatct ctggacgctc tgtaccgaat gccatcgaca aaagtctgct
       61 cgtgaagcgg cgggtggtat gccggacccg acgctgccgg aggtgtccgg aggtcatggc
      121 agggcagacg atatcatcgg actgtgaccc gccccggggg ggggatcatc cggcgaaaaa
      181 aacgatcgct ccggacaccg cgccccctct cacgcagaga aaaaattccc gtttcagggc
      241 agttaacatg ttaactggct gtccgggcat ttttgcggtt tttatcttta ttattcagtt
      301 tgttgtgcgg aaaaaatgtt aactggcttt ttcagcaaat gttaaccagg cagca
//
LOCUS       DAZKFZ010000656          354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000656 DAZKFZ010000000
VERSION     DAZKFZ010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.660"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>354
                     /locus_tag="ACHOQW_005340"
     CDS             <1..>354
                     /locus_tag="ACHOQW_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576183.1"
                     /translation="VKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKFV
                     MNNPEGERADSPKVIIEISLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFERKDES
                     SLHLITNKVLACYQRE"
BASE COUNT          119 a           63 c           74 g           98 t
ORIGIN      
        1 gtaaaaatat ttaccctggg ttatgtggat tttagcaaac gctccaacga agcgggaaga
       61 aatatgatgg ctcatattga gtcctcatct tatatcaaaa ataatgatgg cagtgagata
      121 atgaagtttg ttatgaataa tcctgaaggg gaacgagcgg attcacccaa ggtgattata
      181 gaaatttcac tttccactat tactactatg gggactcgtc aaggacatac agccattata
      241 tttccacaac ctgatggttc gactaaccgt tatgaaagaa agtcctttga aagaaaagat
      301 gagagttcat tacacctgat tactaacaag gttctggcgt gttaccaacg cgaa
//
LOCUS       DAZKFZ010000657          352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000657 DAZKFZ010000000
VERSION     DAZKFZ010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.661"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>352)
                     /locus_tag="ACHOQW_005341"
     CDS             complement(<1..>352)
                     /locus_tag="ACHOQW_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFP9576184.1"
                     /translation="VLYHQLDSDIKERIAFLDISHYAYPGSLLEKLQLSGYVFSDINI
                     IAEYLLASYGVSGHYSHGVVYPAPSDKLLELLRRHTKSNSEWIEKITPYVYDILSDNV
                     SNVLRPPLSEEQKKI"
BASE COUNT          110 a           59 c           63 g          120 t
ORIGIN      
        1 atattttttt ctgttcttct gataaagggg ggcgcaagac gttggataca ttatcggata
       61 atatatcata aacatatggg gttatttttt ctatccactc agaattgctc tttgtatgtc
      121 ttcttaatag ctcaagtaat ttatcagatg gagctggata taccacgccg tgagaataat
      181 gtccagaaac tccgtaacta gccaataagt attctgcaat tatgttgata tcagaaaaaa
      241 cgtagccact taattgtaat ttttctaata aactacccgg atatgcatag tgggatatat
      301 cgagaaatgc tattcgctct ttaatatcac tatctaactg atggtagagt ac
//
LOCUS       DAZKFZ010000658          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000658 DAZKFZ010000000
VERSION     DAZKFZ010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.662"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>343
                     /locus_tag="ACHOQW_005342"
     CDS             <1..>343
                     /locus_tag="ACHOQW_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9576185.1"
                     /translation="LVFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSL
                     SRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAE
                     VLQEDTGVTLPA"
BASE COUNT           79 a           91 c          127 g           46 t
ORIGIN      
        1 gcctggtgtt ccacacgcgg atacagcacg gcgaaccaca ggtggagagc cggtacctct
       61 atgacccgct gggccgccgg acgggaaaac gggtgtggcg gcgggagcgt gacctgacgg
      121 ggtggatgtc gctgtcgcgt aaaccggagg agacctggta cgggtgggac ggtgacaggc
      181 tgaccactgt acagacccaa cagacaagaa tccagacggt ataccagccg ggaagcttca
      241 cgccgctcct gagaatcgaa acagagaatg gtgaacaggc gaaggcgcgg caccgtagcc
      301 tggcggaggt gttgcaggag gacacgggtg tgacgctacc ggc
//
LOCUS       DAZKFZ010000659          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000659 DAZKFZ010000000
VERSION     DAZKFZ010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.663"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(197..>341)
                     /locus_tag="ACHOQW_005343"
     CDS             complement(197..>341)
                     /locus_tag="ACHOQW_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFP9576186.1"
                     /translation="FYRARETDGSASQTFDSGSQGQIQGKITGKDHRCAVGTKMQNGT
                     FCM"
BASE COUNT           76 a          101 c           65 g           99 t
ORIGIN      
        1 aatgcgaatt atttagtaca aaaagtgaac ttttgatgat ctttccctaa tctcacatag
       61 gatcagatct cgtctgatcg ttccccctgc gctcactacc atggctcttt accacagcag
      121 ccggggttgt ttgggaccga cttccgacag cagatcccgg tgggcctgcc accatctctt
      181 cacaagctta tgcttattac atgcaaaacg tcccgttttg catctttgtg cctacggcac
      241 accgatgatc tttccctgtg atctttccct gaatctgccc ctgagaacct gaatcaaacg
      301 tctgactcgc agaaccatct gtttctcgcg ctctgtagaa c
//
LOCUS       DAZKFZ010000660          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000660 DAZKFZ010000000
VERSION     DAZKFZ010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.664"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>341
                     /locus_tag="ACHOQW_005344"
     CDS             <1..>341
                     /locus_tag="ACHOQW_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9576187.1"
                     /translation="VQRGYLDLTEIKQIEIPIEEKEKYQVVKGDLLITEGGDWDTVGR
                     TTVWCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFSRQYFANASKQTTNLASIN
                     KTQLRGCPVAI"
BASE COUNT          124 a           58 c           70 g           89 t
ORIGIN      
        1 cgtccaacgc ggatatcttg atcttacgga aattaaacag attgaaatcc ctattgagga
       61 aaaagaaaaa tatcaagtag tcaagggaga tttattgata acagaaggcg gcgactggga
      121 tacagtcggg agaactacag tatggtgtca tgactggtat atagcaaatc aaaaccatgt
      181 attcaaagga cgaaatatag ggcaagatgt tgatccatat tggttagaaa catatatgaa
      241 tagcccattc tcaagacaat attttgctaa cgcaagtaag caaaccacta atttagcttc
      301 tattaataaa acccagctca gaggttgtcc tgttgctatt c
//
LOCUS       DAZKFZ010000661          336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000661 DAZKFZ010000000
VERSION     DAZKFZ010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.665"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>336)
                     /gene="escQ"
                     /locus_tag="ACHOQW_005345"
     CDS             complement(<1..>336)
                     /gene="escQ"
                     /locus_tag="ACHOQW_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFP9576188.1"
                     /translation="SSQLNMKINDFYLPLLPVIGTGRLYITSKGHACHAYFREVSGHG
                     IRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTALPEGD
                     VSVDDVQFT"
BASE COUNT          103 a           83 c           63 g           87 t
ORIGIN      
        1 atcgtaaatt gaacatcatc aactgacaca tcaccttccg gtaaggcagt ctgcattacc
       61 cataagatca tgagttttat cgtatcttcc ggtatcagcc ggctttcgct atacgggaat
      121 aactcttggc accactgaat gaactgttct tctgatatcc agaaacgccc ctcatagccc
      181 gagtatgtca acgtgaaacg aataccatga ccgctgacct cacgaaaata tgcatggcaa
      241 gcatgacctt tggacgtaat atagaggcga ccggttccga taactggcaa caacggcaaa
      301 taaaaatcgt taattttcat attcaattgt gaactc
//
LOCUS       DAZKFZ010000662          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000662 DAZKFZ010000000
VERSION     DAZKFZ010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.666"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>333)
                     /locus_tag="ACHOQW_005346"
     CDS             complement(<1..>333)
                     /locus_tag="ACHOQW_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576189.1"
                     /translation="ALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSGLERLSN
                     LLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITLQLDLGGESPGYLEK
                     DKHYREADA"
BASE COUNT           88 a           78 c           65 g          102 t
ORIGIN      
        1 cgcatcggct tctctgtaat gtttatcttt ttccagataa cctggggatt ctccccctaa
       61 atcaagctgt aacgttattc tttcataacc tttatctatg aggtggttca acctcgttgt
      121 tgaaagagtt tcatccagtt cattgacttt agcttcgcgt agtaaatcag agacaaaaac
      181 agagtctttc ttcgagagta ggtttgacaa acgttccagt ccagaggtca ccagtgcaac
      241 ggaagatgtg tttaatcggg catcttcctc ccacgctccg gaatcttcca taacatagaa
      301 attggcttta tccagataag caatcagcag cgc
//
LOCUS       DAZKFZ010000663          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000663 DAZKFZ010000000
VERSION     DAZKFZ010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.667"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>333
                     /locus_tag="ACHOQW_005347"
     CDS             <1..>333
                     /locus_tag="ACHOQW_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9576190.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DAZKFZ010000664          327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000664 DAZKFZ010000000
VERSION     DAZKFZ010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.668"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(44..>327)
                     /gene="rfbE"
                     /locus_tag="ACHOQW_005348"
                     /gene_synonym="perA"
     CDS             complement(44..>327)
                     /gene="rfbE"
                     /locus_tag="ACHOQW_005348"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFP9576191.1"
                     /translation="SKDVFHTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHT
                     MPMYSEKYQKHPIAEDLGWRGINLPSFPSLSNEQVIYICESINEFYSDK"
BASE COUNT           98 a           62 c           56 g          111 t
ORIGIN      
        1 aattcaacgc aattttcatg aatgaccttt acaatatttt aggctattta tcactataaa
       61 attcgttaat agattcacaa atataaataa cttgctcatt cgataggctg gggaaactag
      121 gtaaattaat tccacgccaa ccaagatcct cagctatagg gtgcttttga tatttttccg
      181 agtacattgg catcgtgtgg acagggtaaa aaactggcct tgtttcgatg agtttatctg
      241 caaggtgatt ccttaattcc tctctttcct ctgcggtcct agttagaatt gagaccatcc
      301 aataagtgtg aaaaacatct ttacttt
//
LOCUS       DAZKFZ010000665          327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000665 DAZKFZ010000000
VERSION     DAZKFZ010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.669"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>327)
                     /locus_tag="ACHOQW_005349"
     CDS             complement(<1..>327)
                     /locus_tag="ACHOQW_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576192.1"
                     /translation="TDAYNAASSHIEYWIGAGVSFISMMILFRVYDFLLPAQDTFPGD
                     IIRSLCVVCPSILIWFQFSVYVMRKYYRHILERGKETETISERLIREADTREYELWRP
                     ARRFFS"
BASE COUNT          106 a           70 c           65 g           86 t
ORIGIN      
        1 atggagaaaa agcgacgggc cggacgccat agttcatatt ctcgtgtatc agcctcacgt
       61 atcaaccttt ctgaaatggt ttcggtttcc ttacctcgtt ccagaatatg tctgtaatat
      121 ttcctcatga catataccga aaactgaaac cagattaata tggatggaca cacaacacat
      181 aaacttctga tgatatcccc cgggaaagta tcttgggcgg gtaaaagaaa gtcatatact
      241 ctgaataaaa tcatcatcga gataaatgag acgccagcac ctatccagta ttcgatatgg
      301 gaagatgcag cattatatgc gtcagta
//
LOCUS       DAZKFZ010000666          327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000666 DAZKFZ010000000
VERSION     DAZKFZ010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.670"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>327
                     /locus_tag="ACHOQW_005350"
     CDS             <1..>327
                     /locus_tag="ACHOQW_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9576193.1"
                     /translation="STPEGEWYIPCVNISAEPEAYFRIAPEDWLRAEMQGEIVALVHS
                     HPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFRCVPHLTGRRFEHGVTDCYTLFRD
                     AYHLAG"
BASE COUNT           61 a           81 c          116 g           69 t
ORIGIN      
        1 taagcacgcc ggagggggag tggtatatcc cttgtgtgaa tatttctgca gagccggagg
       61 cgtattttcg tatcgcaccg gaagactggc tgcgggcaga gatgcagggg gagattgtgg
      121 cactggtcca cagtcatccc ggtgggctgc cctggctgag cgaggccgac cggcggctgc
      181 agataaaaag cgcactgccc tggtggctgg tctgccgggg tgacattcac aaattccgct
      241 gtgtgccaca tctgacagga cggcgctttg agcacggggt gacggactgt tacacgctgt
      301 tccgggatgc ttatcatctg gcgggga
//
LOCUS       DAZKFZ010000667          325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000667 DAZKFZ010000000
VERSION     DAZKFZ010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.671"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>325
                     /gene="espR3"
                     /locus_tag="ACHOQW_005351"
     CDS             <1..>325
                     /gene="espR3"
                     /locus_tag="ACHOQW_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFP9576194.1"
                     /translation="EGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVK
                     EGASNDLKENVDRALVRMFKCVKLHSNELNLSSLSLGSVPPLPEWIEMLSLVYNELDS
                     IQVPES"
BASE COUNT           95 a           56 c           77 g           97 t
ORIGIN      
        1 gggaggggac tttacccatt cggcataaat tagagtttat atcgaccaat attgcagagt
       61 tgctggacaa gttaaccaaa attacagatg ccaggctttg caaaggtttc agtgactggg
      121 ctagttcagt caaagaaggc gcatcgaatg acttgaaaga aaatgtggat agagcattgg
      181 tgagaatgtt taaatgtgtt aagcttcaca gtaatgaact taacttatca agcctttctt
      241 tgggttctgt gccgcctctt cctgagtgga ttgaaatgct tagccttgtt tataatgaac
      301 ttgattcaat acaggtgccc gaatc
//
LOCUS       DAZKFZ010000668          325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000668 DAZKFZ010000000
VERSION     DAZKFZ010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.672"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(16..>325)
                     /gene="perB"
                     /locus_tag="ACHOQW_005352"
     CDS             complement(16..>325)
                     /gene="perB"
                     /locus_tag="ACHOQW_005352"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFP9576195.1"
                     /translation="STNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVVIED
                     NAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKRSPTSI
                     "
BASE COUNT           95 a           77 c           55 g           98 t
ORIGIN      
        1 gttttcgcat tcccattaaa tagatgttgg cgatcttttc atttctcttg ctggatttcc
       61 gcacacagtt ataccggcag gaacactttt agtgacaaca gcccccatac ctataatggc
      121 tcccgcgcca ataataagtg ggcgattagg aacaccctgc ttaattactg cacccgagcc
      181 tatatatgca ttgtcttcaa taacaacata tccattacat ttagccccag gagcaaatgt
      241 aacatagtct cctatttgac aatcatgtgc aacgtatgag tatatgtttg catgaaaaaa
      301 cctccctata tgagtattag tagat
//
LOCUS       DAZKFZ010000669          324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000669 DAZKFZ010000000
VERSION     DAZKFZ010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.673"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>324
                     /gene="cesT"
                     /locus_tag="ACHOQW_005353"
     CDS             <1..>324
                     /gene="cesT"
                     /locus_tag="ACHOQW_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFP9576196.1"
                     /translation="SLSDANDEYMMIYGVCGKFPTDNSNFALEILNANLWFAENGGPY
                     LCYEAGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKI
                     EEISSS"
BASE COUNT          102 a           54 c           73 g           95 t
ORIGIN      
        1 tcgttatctg atgccaatga cgaatatatg atgatttatg gtgtctgtgg gaaattcccg
       61 acagataact ctaacttcgc tcttgagatt ttgaatgcaa acttatggtt tgcagagaat
      121 ggtgggccat atctgtgcta tgaggctgga gcacaatcgc tgttgttagc gttacgtttc
      181 cctctcgatg atgctacccc tgaaaaactc gagaatgaaa tagaagtcgt tgttaagtca
      241 atggaaaacc tgtatttggt attacataat cagggaataa cattagaaaa cgaacatatg
      301 aaaatagagg aaatcagttc aagc
//
LOCUS       DAZKFZ010000670          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000670 DAZKFZ010000000
VERSION     DAZKFZ010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.674"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>323
                     /locus_tag="ACHOQW_005354"
     CDS             <1..>323
                     /locus_tag="ACHOQW_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576197.1"
                     /translation="EKKIADAGFITRKVKAENFNVKEFKDDIASMLCEQPPMRTHQIS
                     SSGYVSADKLHKLYKTNLEDAQRDLKTTPVDIDGVSLKSITREKIAEEIAKKTAEIIA
                     KQNAK"
BASE COUNT          123 a           59 c           72 g           69 t
ORIGIN      
        1 tagagaagaa aattgccgat gcggggttta taacaaggaa ggttaaagcc gagaacttta
       61 atgtaaaaga gtttaaagac gatatagcct cgatgctttg tgaacagccc cccatgcgta
      121 cacaccagat ttcttcatcc ggatacgtat ctgccgataa actgcacaaa ctctataaga
      181 caaatttgga agacgcgcag agagatctga aaacaacacc ggttgacatc gatggtgtta
      241 gtctaaaatc aataactcgg gaaaaaattg cggaagaaat tgcgaaaaaa actgcggaaa
      301 taattgcgaa acaaaatgcg aag
//
LOCUS       DAZKFZ010000671          308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000671 DAZKFZ010000000
VERSION     DAZKFZ010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.675"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..288
                     /locus_tag="ACHOQW_005355"
     CDS             <1..288
                     /locus_tag="ACHOQW_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576198.1"
                     /translation="VQWEQKFARHVQHVRAQVKPVSKGVSHAAPGGTASRAVQEIRAA
                     REQWERENGFISNGNGLEAVGTYGGGVFEPLDPEERGCAVEALDCSDWRDD"
BASE COUNT           82 a           72 c          103 g           51 t
ORIGIN      
        1 gttcagtggg agcagaaatt tgcccgtcac gtgcagcacg tcagggcaca ggtaaaacca
       61 gtcagcaagg gggtaagcca tgcagcacca ggtggcaccg catcacgggc agttcaggaa
      121 attcgggcag cacgtgaaca gtgggaacgt gaaaacggat ttatcagcaa cggaaacggc
      181 ctggaagctg tgggaactta tgggggaggt gtattcgaac cgctggaccc agaagaacgg
      241 ggctgcgccg tcgaagctct ggattgctca gattggcgcg atgactgaac agcaaatccg
      301 gcaggtct
//
LOCUS       DAZKFZ010000672          307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000672 DAZKFZ010000000
VERSION     DAZKFZ010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.676"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..201
                     /locus_tag="ACHOQW_005356"
     CDS             <1..201
                     /locus_tag="ACHOQW_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576199.1"
                     /translation="LFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPL
                     AYSDYAEGWNDCREAMLQGDKS"
     gene            198..>307
                     /locus_tag="ACHOQW_005357"
     CDS             198..>307
                     /locus_tag="ACHOQW_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576200.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIME"
BASE COUNT           89 a           70 c           77 g           71 t
ORIGIN      
        1 ctgtttaacc ctgacacagt tgaagcatac gcaaaacgtg cggaaatatc acctaaacca
       61 ctattctccg cgccgccagc gctggtagtg cctgataagt tgccgcgtga atacagaaac
      121 ggttggcctc ttgcgtatag tgattatgct gaaggctgga acgactgccg cgaagccatg
      181 cttcagggag ataaatcatg attaatcgta tcaagctgga gcacatcctc gaatatgcca
      241 ggcagcagag gcatattggt cagcattgta aaattccacc aggagatatg gttgaaatca
      301 tggagat
//
LOCUS       DAZKFZ010000673          305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000673 DAZKFZ010000000
VERSION     DAZKFZ010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.677"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..181
                     /locus_tag="ACHOQW_005358"
     CDS             <1..181
                     /locus_tag="ACHOQW_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9576201.1"
                     /translation="CLRKARRTPVSPMNLTEIKQAVNRLHSELGMGFEERRVFRRYKG
                     TGEQNTSGNARSKKC"
BASE COUNT           91 a           56 c           81 g           77 t
ORIGIN      
        1 gtgtttgcgt aaggcgcgcc ggactccggt ttcaccgatg aacctgacag aaataaaaca
       61 agcggtcaac agactacatt ctgagttggg aatgggattt gaagagcggc gggtattccg
      121 acgatataaa gggacaggag aacagaatac gtccggaaac gcgcggtcaa aaaaatgcta
      181 aaaaatatct gagagcgtta ttgcctgtta ccataagaaa aagcgacttt agtggtcgct
      241 ttttgtgtca tatataagtc gtttaagtaa acctgtctga acaggtgctc tggtcgtgtt
      301 tgtct
//
LOCUS       DAZKFZ010000674          304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000674 DAZKFZ010000000
VERSION     DAZKFZ010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.678"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>304)
                     /locus_tag="ACHOQW_005359"
     CDS             complement(<1..>304)
                     /locus_tag="ACHOQW_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9576202.1"
                     /translation="AIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSIN
                     YELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPV"
BASE COUNT           90 a           64 c           50 g          100 t
ORIGIN      
        1 acaggtttaa gtaaatcttc tttactccac ggagaagctg ctttggcgat caaaaaacta
       61 cgtgacaccg gatagtcagc ccactcatgc caaggttttg taggtcccac atagtgtata
      121 aacacggtgt catcattgac tggattatca actttgtctt ttaattcata gttaatacta
      181 tatcgggtat tatatttctc cgaaataaac ttcactttac cattcaataa tacattcagt
      241 acatcttgat caaggtgggt gattttactt acccaatctg ggtcacgcaa catttcaata
      301 gctt
//
LOCUS       DAZKFZ010000675          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000675 DAZKFZ010000000
VERSION     DAZKFZ010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.679"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>301
                     /locus_tag="ACHOQW_005360"
     CDS             <1..>301
                     /locus_tag="ACHOQW_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9576203.1"
                     /translation="KHPDKILPILHGCPVESIEVIKNAKRTGQWSYEMTDKNGQSIEV
                     SYIFTDTIDDKSDGKLCLKICNSCNQNDSSTHEYKIDHQQLKNHIRQLEEWALLEK"
BASE COUNT          109 a           51 c           60 g           81 t
ORIGIN      
        1 taagcatcct gataaaattc tgcccattct tcatggttgt cctgtagagt cgatcgaagt
       61 cattaagaat gcgaaacgaa cgggacaatg gtcatatgag atgactgata agaacgggca
      121 gagtattgaa gtgtcctata ttttcactga taccatagat gataaatctg atggaaaatt
      181 atgcttaaaa atctgtaact cctgcaacca aaatgattct agtacacatg aatataaaat
      241 agatcatcaa cagttgaaaa atcacataag gcaacttgag gagtgggccc ttcttgaaaa
      301 g
//
LOCUS       DAZKFZ010000676          300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000676 DAZKFZ010000000
VERSION     DAZKFZ010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.680"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            68..283
                     /locus_tag="ACHOQW_005361"
     CDS             68..283
                     /locus_tag="ACHOQW_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9576204.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
BASE COUNT           89 a           70 c           70 g           71 t
ORIGIN      
        1 aatgcatttt tctggcagca ggcttcatat tctgtgtgct tatgcttgcc gacatgggac
       61 ttgttcaatg acacctcagc aggaaaacgc ccttcgcagc attgcccgtc aggctaattc
      121 tgaaatcaaa aaagccagac agcagtttcc ggataaaaac gtcgatgaca tttgccgtag
      181 cgtactgaag aagcaccgcg aaacggtaac gctaatggga ttcacaccga ctcatttaag
      241 tctggcgatc ggcatgttaa acggcgtctt taaggagcga tgaacatgaa aagcaaaatc
//
LOCUS       DAZKFZ010000677          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000677 DAZKFZ010000000
VERSION     DAZKFZ010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.681"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
BASE COUNT          114 a           48 c           59 g           70 t
ORIGIN      
        1 gttgatcgga aagatggggg gtatcaatga ttgttgcgct aaacggctgg tgtgatcacg
       61 cgcacattaa tgaaggcata aaaaaaagcc ggcatcatgc cggcccaaaa aatataaaca
      121 agacgataaa aggctaataa cggaagcatc atgacacaga ataataaaaa ggtataattg
      181 taatcagtgc taatatattg attgaatgaa ttaataaagc cgatctcaaa gataaataaa
      241 atcgctcaaa acgatttttc ctgtattcac tgccgttgcg caaaaattat c
//
LOCUS       DAZKFZ010000678          287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000678 DAZKFZ010000000
VERSION     DAZKFZ010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.682"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>287)
                     /locus_tag="ACHOQW_005362"
     CDS             complement(<1..>287)
                     /locus_tag="ACHOQW_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HFP9576205.1"
                     /translation="AARLESGIRISIGSGQYSVHYVQLLDGFSVEPVRGGLLDRLLGR
                     EHRMDRRAVALERQLNGGVDFLSSVNNYFQSVMAEHRENKTGNKILMEKINS"
BASE COUNT           79 a           75 c           37 g           96 t
ORIGIN      
        1 gaatttattt tttccattaa tattttatta cctgttttat tttctctgtg ttctgccatg
       61 acactctgaa aatagttatt aacactactt aaaaaatcga cacctccatt taattgcctt
      121 tccagagcca cagcccttct atccattcga tgctcacgcc ccaatagcct atccagtaag
      181 cctcctctca ccggttcaac tgaaaatcca tccagtagtt ggacatagtg aacagaatat
      241 tggccactac caatggatat tcttattcca ctttcaagtc tggcagc
//
LOCUS       DAZKFZ010000679          278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000679 DAZKFZ010000000
VERSION     DAZKFZ010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.683"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            24..100
                     /locus_tag="ACHOQW_005363"
     tRNA            24..100
                     /locus_tag="ACHOQW_005363"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHOQW_005364"
     tRNA            143..218
                     /locus_tag="ACHOQW_005364"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZKFZ010000680          269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000680 DAZKFZ010000000
VERSION     DAZKFZ010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.684"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..133)
                     /locus_tag="ACHOQW_005365"
                     /pseudo
     CDS             complement(1..133)
                     /locus_tag="ACHOQW_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           82 a           45 c           65 g           77 t
ORIGIN      
        1 cttgggcgat ttttagtgat tgtttaaata gccctgtcat gcagttaaag tggcgggcat
       61 cctcttcaag gatggtgatc ctcaaccgca cccatttagg atgcgactat tgtggtagaa
      121 caatgtaaac cataattaat ctgcgtgcgg atacatgctt catagatagg atattctggt
      181 ttgggttgtg aatgtgttgg tcatcgagtg gacaataagg taagaggagt aactcctgca
      241 aaacaaaaaa agagttcaac aaaatcaat
//
LOCUS       DAZKFZ010000681          266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000681 DAZKFZ010000000
VERSION     DAZKFZ010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.685"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>266
                     /gene="nleE"
                     /locus_tag="ACHOQW_005366"
     CDS             <1..>266
                     /gene="nleE"
                     /locus_tag="ACHOQW_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFP9576206.1"
                     /translation="RGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTP
                     EGSWLTVISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKE"
BASE COUNT           87 a           46 c           59 g           74 t
ORIGIN      
        1 agaggagtag ctgaactaaa acaggaagaa tttgttaacg agaaagccag acggttttct
       61 tatatgaaga ctatgtattc tgtatgtcca gaagcgtttg aacctatttc cagaaatgaa
      121 gccagtacac cggaaggaag ctggctaaca gttatatccg gaaaacgccc aatggggcag
      181 ttttctgtag atagtttata caatcctgat ttacatgcat tatgtgagct tccggacatt
      241 tgttgtaaga tcttccctaa agaaaa
//
LOCUS       DAZKFZ010000682          266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000682 DAZKFZ010000000
VERSION     DAZKFZ010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.686"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..>266
                     /locus_tag="ACHOQW_005367"
     CDS             <1..>266
                     /locus_tag="ACHOQW_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9576207.1"
                     /translation="KVSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLAL
                     ENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPE"
BASE COUNT           75 a           74 c           57 g           60 t
ORIGIN      
        1 ccaaagtatc agatatgcag aaaaatgtga ctcccggcag gcgaaaaggc tgccctaatt
       61 atcctcccga atttaaacag cagctcgttg ctgcctcctg tgaacccggg atatccatct
      121 caaaacttgc tcttgaaaat ggcattaacg ccaatctgtt gttcaaatgg cgacaacaat
      181 ggcgcgaggg aaagctgcta ttaccttctt cagagagccc ccagctactt cctgtgactc
      241 tcgatgcagc tgccgaacag ccagaa
//
LOCUS       DAZKFZ010000683          265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000683 DAZKFZ010000000
VERSION     DAZKFZ010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.687"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(1..179)
                     /locus_tag="ACHOQW_005368"
                     /pseudo
     CDS             complement(1..179)
                     /locus_tag="ACHOQW_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
BASE COUNT           77 a           70 c           27 g           91 t
ORIGIN      
        1 gaatttattt tttccattaa tattttatta cttgttttat tttctctgtg ttctgccatg
       61 acactctgaa aatagttatt aacactcctt aaaaaatcga cacctccatt taattgcctt
      121 tccagagcca cagcccttct ctccattcga ttctcacgcc ccaatagcct atccagtaga
      181 tttgccccta tatttccaga cacctgttat taaatattca ttccaaacac agggagtcat
      241 aacaaacatc caccgtacat gacaa
//
LOCUS       DAZKFZ010000684          262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000684 DAZKFZ010000000
VERSION     DAZKFZ010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.688"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..>262)
                     /locus_tag="ACHOQW_005369"
     CDS             complement(<1..>262)
                     /locus_tag="ACHOQW_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9576208.1"
                     /translation="GGEIIDTDARTELGEGVLHIAVRKRRQLDVRCFIQHGADINAKA
                     NNGFDFTPLHYSIGIGDFDMMKLLLKLGADIYLESEMGYSPMD"
BASE COUNT           83 a           70 c           31 g           78 t
ORIGIN      
        1 aatccatagg ggagtatccc atctcactct ccaaataaat atcagcacct aatttcaaca
       61 aaagcttcat catatcaaaa tcccctatcc ctatcgaata atgcaaagga gtgaagtcaa
      121 agccattatt tgccttagca tttatatcgg caccatgttg aataaaacat ctgacatcta
      181 attgtcttct tttcctaaca gctatatgaa gaaccccctc gccaagttct gtccttgcat
      241 ctgtatctat aatctctcca cc
//
LOCUS       DAZKFZ010000685          257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000685 DAZKFZ010000000
VERSION     DAZKFZ010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.denovo.689"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            complement(<1..226)
                     /locus_tag="ACHOQW_005370"
     CDS             complement(<1..226)
                     /locus_tag="ACHOQW_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP9576209.1"
                     /translation="MLNGISNAASTLGRQLVGIASRVSSAGGTGFSVAPQAVRLTPVK
                     VHSPFSPGSSNVNARTIFNVSSQVTSFTPSR"
BASE COUNT           75 a           64 c           59 g           59 t
ORIGIN      
        1 gacgagaggg agtaaatgaa gtcacctggc tgctcacatt aaaaatcgtt ctcgcattaa
       61 cattcgacga gcctggagaa aaaggggaat gaactttcac cggagtaaga cgcacggcct
      121 gaggggctac agaaaatcca gttccccccg cagagctcac tcgacttgcg atacctacaa
      181 gctgccgccc tagtgtagaa gcagcgttac taattccatt aagcataatt tatgcctctt
      241 tatatctaag cttggct
//
LOCUS       DAZKFZ010000686         5421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.guided.261, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000686 DAZKFZ010000000
VERSION     DAZKFZ010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.guided.261"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            99..302
                     /locus_tag="ACHOQW_005371"
                     /pseudo
     CDS             99..302
                     /locus_tag="ACHOQW_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            301..822
                     /gene="prfH"
                     /locus_tag="ACHOQW_005372"
                     /pseudo
     CDS             301..822
                     /gene="prfH"
                     /locus_tag="ACHOQW_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHOQW_005373"
     CDS             complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHOQW_005373"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9576210.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2597..3055
                     /gene="gpt"
                     /locus_tag="ACHOQW_005374"
     CDS             2597..3055
                     /gene="gpt"
                     /locus_tag="ACHOQW_005374"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9576211.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3147..4391
                     /gene="frsA"
                     /locus_tag="ACHOQW_005375"
     CDS             3147..4391
                     /gene="frsA"
                     /locus_tag="ACHOQW_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9576212.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4449..4850
                     /gene="crl"
                     /locus_tag="ACHOQW_005376"
     CDS             4449..4850
                     /gene="crl"
                     /locus_tag="ACHOQW_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9576213.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4889..>5421)
                     /locus_tag="ACHOQW_005377"
     CDS             complement(4889..>5421)
                     /locus_tag="ACHOQW_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9576214.1"
                     /translation="NENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNL
                     QSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFD
                     FGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKL
                     NINNDDIVAVGMTYQF"
BASE COUNT         1314 a         1338 c         1407 g         1362 t
ORIGIN      
        1 tgaatcccca ggagcttaca taagtaagtg actggggtga acgaacgcag tcgcagtaca
       61 tgcaacttga agtatgacga gtataaatgg aatggtacat gggcaaatat attcgtccct
      121 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
      181 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
      241 tacaccccgg acgcggctac ccgattggcg cagcgttctt ctctgttggt cgtttttacc
      301 cgacaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc tgacacgctg
      361 cgttcggcgc tgatttctct ggatggcgac aacgcatggg cgttaagcga aagttggtgc
      421 ggcactattc agtggatttg tctgagtccg tatcggcctc atcatgggcg caaaaactgg
      481 tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc aatccgttat
      541 gagacgctgc gtccgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
      601 cgcgccacgc atctggcaac cgggattagc gtgaaggttc agtcagaacg cagccagcat
      661 gctaacaaac ggctggcgcg attgctgatt gcctggaagc tggagcagca gcaacaggaa
      721 aatagcgcag tgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
      781 ccgcgacgaa cgttcacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg
      841 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
      901 agcagttcag tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc
      961 ggagagtgtg gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt
     1021 tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg
     1081 aacaggcgct gataggtttc acgtaccaga tgcatcaccg gagaattagc gtccggctgc
     1141 cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc
     1201 agcatgctca ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt
     1261 tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     1321 tttgccacgt cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag
     1381 gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc cagcaacaag
     1441 gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc
     1501 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     1561 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc cagttcttct
     1621 gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt
     1681 tccccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     1741 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa
     1801 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     1861 cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     1921 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     1981 ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata
     2041 tatggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc
     2101 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     2161 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     2221 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     2281 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     2341 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     2401 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     2461 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     2521 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     2581 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     2641 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     2701 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     2761 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     2821 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     2881 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa
     2941 accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat
     3001 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     3061 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgagtta caatagtttc
     3121 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     3181 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     3241 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     3301 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     3361 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     3421 accgtggcgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
     3481 cctgtgcgga agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt
     3541 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     3601 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctacc gggcacgatg cgacagatgg
     3661 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     3721 gcccgttccc gacggtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     3781 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
     3841 cggtgggctt ttcttcaaaa tggaagctca cccaggactc ctgcctgttg catcagcgcg
     3901 tcttaaaggc tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     3961 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     4021 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     4081 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
     4141 acgaagcgtt gcgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac
     4201 gtcgatgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     4261 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     4321 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac
     4381 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
     4441 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     4501 tactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     4561 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     4621 tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     4681 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
     4741 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagt
     4801 tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg
     4861 ccggatgcat cacatccggc aataatcatt aaaactgata ggtcatgcca accgcgacaa
     4921 tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa
     4981 caaacgctga catatttttg ttgaaataat atgtagcccc gacatcgata taattgacca
     5041 gatcttcatc accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg
     5101 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
     5161 caaagccgcc agttattggc gtcattttgc gtgtttcaga ataaaaagtt gccagataaa
     5221 tattattggc atcgtatttc agacctgtag cccaagcttc tgcacgcttg cctgtgccac
     5281 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
     5341 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
     5401 taacgtcgcg gttttcgttt t
//
LOCUS       DAZKFZ010000687         4480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.guided.285, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000687 DAZKFZ010000000
VERSION     DAZKFZ010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.guided.285"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            29..1162
                     /gene="ampC"
                     /locus_tag="ACHOQW_005378"
     CDS             29..1162
                     /gene="ampC"
                     /locus_tag="ACHOQW_005378"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9576215.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1250..1783
                     /gene="blc"
                     /locus_tag="ACHOQW_005379"
     CDS             1250..1783
                     /gene="blc"
                     /locus_tag="ACHOQW_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9576216.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1780..2097)
                     /gene="sugE"
                     /locus_tag="ACHOQW_005380"
     CDS             complement(1780..2097)
                     /gene="sugE"
                     /locus_tag="ACHOQW_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9576217.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2273..2419)
                     /gene="ecnB"
                     /locus_tag="ACHOQW_005381"
     CDS             complement(2273..2419)
                     /gene="ecnB"
                     /locus_tag="ACHOQW_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9576218.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2530..2655)
                     /gene="ecnA"
                     /locus_tag="ACHOQW_005382"
     CDS             complement(2530..2655)
                     /gene="ecnA"
                     /locus_tag="ACHOQW_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9576219.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2707..3273)
                     /gene="efp"
                     /locus_tag="ACHOQW_005383"
     CDS             complement(2707..3273)
                     /gene="efp"
                     /locus_tag="ACHOQW_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9576220.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3315..4343
                     /gene="epmB"
                     /locus_tag="ACHOQW_005384"
     CDS             3315..4343
                     /gene="epmB"
                     /locus_tag="ACHOQW_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9576221.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1146 a         1139 c         1118 g         1077 t
ORIGIN      
        1 gggccgtttt gtatggaaac cagaccctat gttcaaaacg acgctctgcg ccttattaat
       61 taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac
      121 aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca
      181 gggtaaacct tattacttta cctgggggta tgcggacatc gccaaaaagc agcccgtcac
      241 acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg
      301 cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga
      361 acttaccgct aaacagtgga atgggatcac actattacat ctcgcaacct acactgctgg
      421 cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta
      481 tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat
      541 cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca
      601 aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac cgcccgcaga
      661 agaaaagaat tacgcctggg gatatcgcga aggcaaggca gttcatgttt cgccaggggc
      721 gttagatgct gaaacttatg gtgtgaagtc gaccattgaa gatatggcct gctgggtacg
      781 aagcaatatg aatccccgtg atatcaacga caaaacactt cagcaaggga tacaactggc
      841 acaatctcgc tactggcaaa ccggcgatat gtatcagggt ctgggctggg aaatgctgga
      901 ctggccggta aatcctgaca tcatcgttaa cggcagtgac aataaaattg cactggcagc
      961 acgccccgta aaagcgatta cgcctccaac tcctgcagta cgagcatcat gggtgcataa
     1021 aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg
     1081 tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctggca
     1141 gattctcaac gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc
     1201 ttttccttgc tgtcatctac acttagaaaa aaccagtaag gaaacattta tgcgcctgct
     1261 ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc
     1321 gccgcgtggc gtgaccgtag tgaataattt cgacgctaaa cgctatctcg gtacctggta
     1381 tgagattgcc cgttttgatc accgctttga acgtggactg gaaaaagtca ccgcaacata
     1441 cagcctgcgt gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagagg
     1501 aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct
     1561 gaaagtgtca ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga
     1621 ataccgccat gcgctggttt gcggaccgga ccgcgactac ctgtggatac tctcccgcac
     1681 gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt
     1741 tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag
     1801 accaataatc cccaatacga ttaacgccag actcgccaga cgcatcgggt tagcggactc
     1861 accgagcagc acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc
     1921 ataagccgtc cctactggta acgatttcat cgcccaggca agtagcgcca tactgacaat
     1981 catcgccgtc acggtaataa cactcggcgt caaacgacta aagccgtggg tatatttcag
     2041 gcctacggcc cataccactt ccagcagacc agcaataact aagataatcc aggacatatc
     2101 aggctccgga gaattggggc cgtccccggt gaaaagacgc gtttgcaggt cgtcctgcaa
     2161 agctgatggt tgaacggata ttttgccagg tggcggggga atttcaacta ttacctttgt
     2221 gactatcgca gaaaaaacaa acggcacgac acagctgtcg taccgttatt gcttattgct
     2281 gcgctttcgt tgctgcacca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc
     2341 cacgcgtggt gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg
     2401 caattgtctt cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata
     2461 gcaaattaat aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggaaga
     2521 gaagaaaaat tagctggcag cgcgggagat ggagttgccg agatgtttga tgtcttcgcc
     2581 gaaaccgcga gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat
     2641 aaggcgtttc atcatctctg acctgaataa gtgatggtgc agcctgcagg ccgcaccaca
     2701 accgcattac ttcacgcgag agacgtattc accagagcgg gtatccactt tgatgacttc
     2761 gccgatttgt acgaacagcg gaactttaac cacagcgcca gtagacaggg tagccggttt
     2821 gccgccagta cctgcggtat cacctttcag gcccggatcg gtatcaacga tttccagttc
     2881 aacgaagttc ggcggagtaa cggagatcgg ctgaccattc cacagagtta cgatacactc
     2941 tgcctgatcc agcagccatt tagcgttgtc gccaattgct tttgcgtcag cagacagctg
     3001 ctcgaaagtt tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag
     3061 gttcatatcg acaacatcag cgccttcagc ggaatcagta gatttgaagg ttttttctac
     3121 gcgagtaccg gtcagcagac gacgcagttt aacgcgagca aatgcctggc ctttacccgg
     3181 ttttacgaat tcactcgctt caaccgcgta aggttcgccg tctaacatga ttttaagacc
     3241 agcacgaaaa tcgttgctat agtacgttgc cataaggccc tctgaaattt gttaattggt
     3301 agctaagcca caaaatggcg catattgtaa ccctaaatac cccatccaga gaagattggt
     3361 taacgcaact tgccgatgtt gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag
     3421 acgcggacga aaaactgttg gccggacgca acgccaaaaa gctgtttgcc ctgcgtgtgc
     3481 cccgctcatt tatcgatcgc atggagaaag gcaatccgga cgatccgctt ttgcgtcagg
     3541 tacttacctc gcaagatgag tttgtcgtcg cgcccggatt ctccaccgac ccgctggaag
     3601 aacagcacag cgtagtgcct ggtttgttgc ataaatacca caaccgggcg cttttgctgg
     3661 tcaaaggcgg ctgcgcggta aattgccgct attgcttccg tcgccacttc ccctatgccg
     3721 aaaatcaggg caacaagcgt aactggcaaa ctgcacttga gtatgttgct gcgcatccgg
     3781 aactggacga gatgattttc tccggcggcg atccgctgat ggcgaaagat cacgagctgg
     3841 actggttgct cacacaactg gaagccatcc cgcatatcaa acgtctgcgg attcacagtc
     3901 gtctgccgat tgtgatcccg gcacgcatca cagatgcgct ggttgaacgc ttttcacatt
     3961 ctacgctgca aatcttgctg gtgaatcaca tcaaccatgc caatgagata gatgaaacat
     4021 tccgtcaggc gatggctaag ttgcgccggg cgggcgttac tttgctgaac cagagcgttc
     4081 tgttacgtgg tgtgaacgat aacgcacaaa cgctggcaaa cctgagtaat gcgttgttcg
     4141 atgcaggcgt gatgccgtat tacctgcatg tgctcgataa agtacagggc gcggcgcatt
     4201 ttatggtgag tgatgacgaa gcacggcaga ttatgcgtga gttgctgaca ctggtgtcgg
     4261 gatatctggt gccgaaactg gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg
     4321 atctccagct acgccagcag taactgatta aacagggaag tttaatcttt gcccgttaca
     4381 taacctttaa ttattcatat acattgctgt tgttactctt tatataacca acaccaataa
     4441 ttaacctttc ttttctgttt atatagagtg agtacggcta
//
LOCUS       DAZKFZ010000688         2748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.guided.350, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000688 DAZKFZ010000000
VERSION     DAZKFZ010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.guided.350"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            <1..110
                     /locus_tag="ACHOQW_005385"
     CDS             <1..110
                     /locus_tag="ACHOQW_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9576222.1"
                     /translation="GFWLDDAQVVDYRVVKMPVTKGGRLELTITEMGNE"
     gene            107..247
                     /locus_tag="ACHOQW_005386"
     CDS             107..247
                     /locus_tag="ACHOQW_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9576223.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            333..767
                     /locus_tag="ACHOQW_005387"
     CDS             333..767
                     /locus_tag="ACHOQW_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9576224.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1274..2221
                     /gene="stxA1a"
                     /locus_tag="ACHOQW_005388"
     CDS             1274..2221
                     /gene="stxA1a"
                     /locus_tag="ACHOQW_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9576225.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2231..2500
                     /gene="stxB1a"
                     /locus_tag="ACHOQW_005389"
     CDS             2231..2500
                     /gene="stxB1a"
                     /locus_tag="ACHOQW_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9576226.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          720 a          492 c          706 g          830 t
ORIGIN      
        1 caggtttctg gctggatgat gctcaggtcg ttgattaccg cgttgtgaag atgcctgtta
       61 ccaaaggtgg gaggctggaa ctgaccatca ccgaaatggg gaatgaatga tgtttgagtt
      121 taatatggca gaacttcttc gccaccgctg ggggcgtctg cgcttatatc gtttccccgg
      181 ttctgttttg accgattacc gaatactgaa gaattacgcc aaaaccctga caggagcagg
      241 agtatgaagt cagagataac aatcaactaa tactgttttg ttgatttttg cttgtaattg
      301 gcgttctggt ctgatttttg tggagtaagt tgatgcgtga tattcagatg gttcttgagc
      361 gttggggagc gtgggcggct aataatcatg aagatgtgac ctggtcgtcc attgccgccg
      421 gttttaaggg actgatcccc gaaaaagtaa aatcacgccc gcagtgctgt gacgatgatg
      481 cgatggtgat atgcgggtgt atagcccgcc tttaccggaa caatcgcgat ctgcatgact
      541 tgctggttga ttattacgtg ttgggggaga cgttcatggc gctggcacgg aaacatgggt
      601 gctctgacac ctgtataggt aaacgccttc acaaagcgga ggggattgtt gaaggcatgc
      661 tgatgatgct gggagtgagg cttgagatgg atcggtatgt tgagcgtgaa ttgccgggag
      721 ggagaacctc tgtattttat cagcgaaaaa atagtttacg atcgtaaaaa tctgcatatc
      781 atgataagag tggttacatt gccacgctgc ttaacccgcc gatgcgcggg tttttttgta
      841 cccagaatcc tgtgagctat acggaaagta cacagaaagg aaggtgcgac cacaattaat
      901 aacaaaatct taaaaattgc acatggcact attagttttc taaatattgt gtattttttg
      961 tattgcagga tgaccctgta acgaagtttg cgtaacagca ttttgctcta cgagtttgcc
     1021 agcctccccc agtggctggc ttttttatgt ccgtaacatc ctgtgtatca ataaatgttg
     1081 ttatctacgt acgtcaagta gtcgcatgag atctgaccag atatgttaag gttgcagctc
     1141 tctttgaata tgattatcat tttcattacg ttattgttac gtttatccgg tgcgccgtaa
     1201 aacgccgtcc ttcagggcgt ggaggatgtc aagaatatag ttatcgtatg gtgctcaagg
     1261 agtattgtgt aatatgaaaa taattatttt tagagtgcta acttttttct ttgttatctt
     1321 ttcagttaat gtggtggcga aggaatttac cttagacttc tcgactgcaa agacgtatgt
     1381 agattcgctg aatgtcattc gctctgcaat aggtactcca ttacagacta tttcatcagg
     1441 aggtacgtct ttactgatga ttgatagtgg cacaggggat aatttgtttg cagttgatgt
     1501 cagagggata gatccagagg aagggcggtt taataatcta cggcttattg ttgaacgaaa
     1561 taatttatat gtgacaggat ttgttaacag gacaaataat gttttttatc gctttgctga
     1621 tttttcacat gttacctttc caggtacaac agcggttaca ttgtctggtg acagtagcta
     1681 taccacgtta cagcgtgttg cagggatcag tcgtacgggg atgcagataa atcgccattc
     1741 gttgactact tcttatctgg atttaatgtc gcatagtgga acctcactga cgcagtctgt
     1801 ggcaagagcg atgttacggt ttgttactgt gacagctgaa gctttacgtt ttcggcaaat
     1861 acagagggga tttcgtacaa cactggatga tctcagtggg cgttcttatg taatgactgc
     1921 tgaagatgtt gatcttacat tgaactgggg aaggttgagt agcgtcctgc ctgactatca
     1981 tggacaagac tctgttcgtg taggaagaat ttcttttgga agcattaatg caattctggg
     2041 aagcgtggca ttaatactga attgtcatca tcatgcatcg cgagttgcca gaatggcatc
     2101 tgatgagttt ccttctatgt gtccggcaga tggaagagtc cgtgggatta cgcacaataa
     2161 aatattgtgg gattcatcca ctctgggggc aattctgatg cgcagaacta ttagcagttg
     2221 agggggtaaa atgaaaaaaa cattattaat agctgcatcg ctttcatttt tttcagcaag
     2281 tgcgctggcg acgcctgatt gtgtaactgg aaaggtggag tatacaaaat ataatgatga
     2341 cgataccttt acagttaaag tgggtgataa agaattattt accaacagat ggaatcttca
     2401 gtctcttctt ctcagtgcgc aaattacggg gatgactgta accattaaaa ctaatgcctg
     2461 tcataatgga gggggattca gcgaagttat ttttcgttga ctcagaatag ctcagtgaaa
     2521 atagcaggcg gagattcata aatgttaaat acatctcaat tcagtcagtt gttgccggtc
     2581 tgataataga tgtgttagaa aatttctgca tggtgaatcc ccctgtgcgg aggggcgact
     2641 ggtgaacggt atgatctctt tgatgatcgt aagcgagaat acgcgggttt ggtggcacca
     2701 ggccgaactc accgggaggc acccggcatc atgctgtata cagagatt
//
LOCUS       DAZKFZ010000689         2115 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T21822
            SAMN43283566-rid23084493.guided.377, whole genome shotgun sequence.
ACCESSION   DAZKFZ010000689 DAZKFZ010000000
VERSION     DAZKFZ010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283566
            Sequence Read Archive: SRR30325514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351776.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351776.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:53:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,305
            Genes (coding)                    :: 5,074
            CDSs (with protein)               :: 5,074
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 96 of 231
            Pseudo Genes (incomplete)         :: 126 of 231
            Pseudo Genes (internal stop)      :: 56 of 231
            Pseudo Genes (multiple problems)  :: 38 of 231
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283566-rid23084493.guided.377"
                     /serotype="O157:H 7"
                     /isolate="2211T21822"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2c"
     gene            95..1054
                     /locus_tag="ACHOQW_005390"
     CDS             95..1054
                     /locus_tag="ACHOQW_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFP9576227.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     DSQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1066..1335
                     /locus_tag="ACHOQW_005391"
     CDS             1066..1335
                     /locus_tag="ACHOQW_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFP9576228.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1632..1955)
                     /locus_tag="ACHOQW_005392"
     CDS             complement(1632..1955)
                     /locus_tag="ACHOQW_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP9576229.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          557 a          412 c          511 g          635 t
ORIGIN      
        1 cacacttatt ttccctggct cgcttttgcg ggcctttttt atatctgcgc cgggtctggt
       61 gctgattact tcagccaaaa ggaacacctt gtatatgaag tgtatattat ttaaatgggt
      121 actgtgcctg ttactgggct tttcttcggt atcctattcc cgggaattta cgatagactt
      181 ttcgactcaa caaagttatg tatcttcgtt aaatagtata cggacagaga tatcgacccc
      241 tcttgaacat atatctcagg ggaccacatc ggtgtctgtt attaaccaca ccccaccggg
      301 cagttatttt gctgtggata tacgagggct tgatgtctat caggcgcgtt ttgaccatct
      361 tcgtctgatt attgagcaaa ataatttata tgtggccggg ttcgttaata cggcaacaaa
      421 tactttctac cgtttttcag attttacaca tatatcagtg cccggtgtga caacggtttc
      481 catgacaacg gacagcagtt ataccactct gcaacgtgtc gcagcgctgg aacgttccgg
      541 aatgcaaatc agtcgtcact cactggtttc atcatatctg gcgttaatgg agttcagtgg
      601 taatacaatg accagagatg catccagagc agttctgcgt tttgtcactg tcacagcaga
      661 agccttacgc ttcaggcaga tacagagaga atttcgtcag gcactgtctg aaactgctcc
      721 tgtgtatacg atgacgccgg gagacgtgga cctcactctg aactgggggc gaatcagcaa
      781 tgtgcttccg gagtatcggg gagaggatgg tgtcagagtg gggagaatat cctttaataa
      841 tatatcggcg atactgggca ctgtggccgt tatactgaat tgtcatcatc agggggcgcg
      901 ttctgttcgc gccgtgaatg aagatagtca accagaatgt cagataactg gcgacaggcc
      961 cgttataaaa ataaacaata cattatggga aagtaataca gctgcagcgt ttctgaacag
     1021 aaagtcacag tttttatata caacgggtaa ataaaggagt taagtatgaa gaagatgttt
     1081 atggcggttt tatttgcatt agtttctgtt aatgcaatgg cggcggattg cgctaaaggt
     1141 aaaattgagt tttccaagta taatgagaat gatacattca cagtaaaagt ggccggaaaa
     1201 gagtactgga ccagtcgctg gaatctgcaa ccgttactgc aaagtgctca gttgacagga
     1261 atgactgtca caatcaaatc cagtacctgt gaatcaggct ccggatttgc tgaagtgcag
     1321 tttaataatg actgaggcat aacctgattc gtggtatgtg ggtaacaagt gtaatctgtg
     1381 tcacaattca gtcagttgac agttgcctgt cagactgagc attcgttaaa aaaattcgca
     1441 tggtgaatcc ccctgtgcgg aggggcgact ggtgtataag gtgtcatctc tgacgacaac
     1501 ctggagcgag atacgcgggt tcagggacac cggactgaac tcaccgggag gcacccggca
     1561 ccatgtacat gatgatacag atacgcagtg tcagcccctc tccggagggg ctttttatct
     1621 gaatgattct gttattttcg cccgtatggg catatttcca gaatgcagcg atgattaaaa
     1681 cacttatctg tgatatttcc tgtgtttgca gggcactcct ggctgttttt aattaaattc
     1741 cagacgtttt tattaaatgg ggccacgttg taaatggtga cggggagctc tttattattg
     1801 agtattacgc ctgtccgggt tggtgtaaaa atacagcccg gagggaggaa ggtatctgac
     1861 tgataccata atattaattt tatgttacac catatggctg aaaatgatat gccacatgct
     1921 ggctggatta ccgtgtcaat cactatccag ttcatttgct ttccttattt tcttaaggtt
     1981 ttaaatgatg ccataaatta taggtgatgt tttgaaagca atctgtcagg ctgagttgtt
     2041 tttctggtgt cgtttattct ttgtttaaaa taagagggca ttttaaattg tcggcgtttt
     2101 tttgttatat aagag
//
